Patent ID: 7144740
Filing Date: 2006-12-05
Classification: B01J,F02M,F23C,G01N

Abstract:
1. A method for detection and identification of constituents of extracts from plants or animals, natural or synthetic sources possessing medicinal value, using chromatographic finger printing techniques, the method comprising the steps of: i. extracting organic or organo-metallic compounds from plants or animal, natural or synthetic sources using a solvent; ii. subjecting the extract obtained in step I, to separating based on pH and polarity, using High Pressure Liquid Chromatography (HPLC) ultraviolet-visible (UV-Vis) 3-D chromatography technique; iii. generating contour and 3-D chromatograms of the constituents eluted in step ii, based on the pH and polarity; iv. converting the 3-D and contour chromatogram obtained into a colored image, analyzing the colored image for its individual colors using the co-ordinates denoting all its 3-dimension properties of said image; v. denoting the concentrations of the various constituents eluted with time; vi. generating a chromatogram based on color analyzed, having peaks at various retention times along with conjugative properties of the constituents; vii. identifying the compounds in the said ingredients by the UV-Vis electromagnetic radiation absorptive properties of the various constituents in the image, thus creating a chromatographic fingerprint of the extract; viii. identifying, determining and classifying the compounds eluted as polar, medium polar and less or non-polar based on the polarity and conjugative properties; ix. generating a barcode for a selected peak using X-axis as Retention Time, the Y-axis as Wavelength, R as the number of Red Pixels, G as the number of Green Pixels and B as the number of Blue Pixels; and x. generating a database of fingerprints and barcodes and identifying the respective compounds of the extract.