Patent ID: 6689574
Filing Date: 2004-02-10
Classification: G01N

Abstract:
A method of identifying an agonist of a nuclear receptor that comprises providing:(a) a nuclear receptor or ligand binding domain thereof labeled with a first fluorescent reagent; (b) CBP, p300, or other nuclear receptor co-activator, or a binding portion thereof, labeled with a second fluorescent reagent; and (c) a substance suspected of being an agonist of the nuclear receptor; under conditions such that, if the substance is an agonist of the nuclear receptor, binding between the nuclear receptor or ligand binding domain thereof and CBP, p300, or other nuclear receptor co-activator, or a binding portion thereof, will occur; and (d) measuring fluorescence resonance energy transfer (FRET) between the first and second fluorescent reagents; where the occurrence of FRET indicates that the substance is an agonist of the nuclear receptor.