Patent ID: 6410341
Filing Date: 2002-06-25
Classification: G01N,Y10S

Abstract:
An analytical test device suitable for determining the presence of at least one analyte contained in a sample while permitting rapid and efficient flow of the sample through at least one defined pathway in which reactions determinative of the analyte take place, said device comprising:a top layer having an upper surface formed with a through hole for the addition of the sample, said through hole being in registry with a sample delivery channel formed in a lower surface of the top layer, said sample delivery channel having walls defined by said lower surface of said top layer and an upper surface of a bottom layer, said sample delivery channel in operative communication with a sample circulation channel closed at its terminal ends and formed with inner and outer walls to define a pathway for the sample in the lower surface of the top layer, the inner walls of the circulation channel defining an indent in the lower surface of the top layer; the bottom layer attached to the top layer, a dry porous carrier therebetween in contact with said walls of said sample circulation channel and providing a fluid pathway therefrom; said fluid pathway of said dry porous carrier layer configured to contain at least one detection zone having a border in operative communication with the sample circulation channel, the sample being permitted to enter into said detection zone from said sample circulation channel from a plurality of different directions; and an opposite end in operative communication with a capture zone channel having an entrance end and a closed terminal end to provide a conduit through which the liquid sample may flow by capillary action from the sample circulation channel to the terminal end of the capture zone channel, the distances between all points where the sample is permitted to enter the detection zone and said entrance end being essentially the same; the sample delivery channel, sample circulation channel, detection zone and capture zone channel forming a defined pathway through which the liquid sample flows from the through hole to the terminal end of the capture channel; there being immobilized reagent in the capture zone channel which will react with said analyte to form a detectable product.