Patent ID: 9133477
Filing Date: 2015-09-15
Classification: A01K,C12N

Abstract:
1. A gene expression system comprising a coding sequence to be expressed in an insect, a promoter operably linked thereto, and an intronic splice control sequence, which is flanked on its 5′ end by a guanine (G) nucleotide, wherein said intronic splice control sequence, in cooperation with a spliceosome, is capable of sex-specifically mediating alternative splicing of RNA transcripts of the coding sequence, the coding sequence encodes a protein having a lethal, deleterious or sterilizing effect and is not a sequence associated with or linked to the intronic splice control sequence in a native context, wherein the intronic splice control sequence is obtained from AaActin-4, Dsx, Bztra or Cctra, and wherein the insect is selected from the group consisting of mosquito, bollworm and medfly.