Patent ID: 6682905
Filing Date: 2004-01-27
Classification: C07K,C12N

Abstract:
A method for identifying a compound that modulates the phosphotyrosine phosphatase activity of a polypeptide, comprising:(a) incubating the compound with the polypeptide, wherein the polypeptide is in pure form, in a membrane preparation or in a whole cell; (b) measuring the phosphotyrosine phosphatase activity of the polypeptide; and (c) comparing the phosphotyrosine phosphatase activity in (b) to that of the polypeptide incubated without the compound, thereby determining whether said compound modulates the phosphotyrosine phosphatase activity of the polypeptide, wherein the polypeptide has phosphotyrosine phosphatase activity and comprises: i) the amino acid sequence SEQ ID NO:1; ii) an amino acid sequence encoded by a nucleic acid molecule that hybridizes at 42Â° C. in 50% formamide, 5Ã—SSC, 25 mM KPO4, 5Ã—Denhardt's, 10 &mgr;g/ml salmon sperm DNA and 10% sulfate followed by washing at 58Â° C. in 0.1Ã—SSC and 0.1% SDS to the complement of a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO: 2; iii) amino acid residues 1-500, 19-500, 151-500, 176-500, 265-500, 558-790 or 265-790 of SEQ ID NO: 1; iv) the amino acid sequence of SEQ ID NO: 3; v) an amino acid sequence encoded by a nucleic acid molecule that hybridizes at 42Â° C. in 50% formamide, 5Ã—SSC, 25 mM KPO4, 5Ã—Denhardt's, 10 &mgr;/ml salmon sperm DNA and 10% sulfate followed by washing at 58Â° C. in 0.1Ã—SSC and 0.1% SDS to the complement of a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:4; or vi) amino acid residues 1-486, 20-486, 143-486, 167-486, 259-486, 552-776 or 259-776 of SEQ ID NO:3.