Patent ID: 6917882
Filing Date: 2005-07-12
Classification: A61K,C07K,C12N

Abstract:
1. A method of identifying a set of oligonucleotides for use in a physical recombination procedure, the method comprising: (a) providing two or more parental character strings representing two or more polynucleotides or polypeptides, which character strings, when aligned for maximum identity, comprise at least one region of heterology; (b) aligning the parental character strings to identify one or more regions of pairwise homology; (c) manipulating the two or more parental character strings by applying a crossover operator to a point selected outside an identified pairwise homology region to thereby computationally generate one or more derivative character strings; and (d) from the one or more derivative character strings, computationally selecting a set of character substrings having sequences that identify the set of oligonucleotides for in vitro recombination, (e) synthesizing the set of oligonucleotides selected from the derivative character strings to be used for a physical recombination procedure.