Patent ID: 7645569
Filing Date: 2010-01-12
Classification: C12N,C12Q,G01N

Abstract:
1. A method of detecting binding of a PAS (Per-ARNT-Sim) domain of a protein with a foreign core ligand of the PAS domain, wherein the PAS domain is prefolded in its native state, the method comprising the steps of: determining from NMR analysis of the PAS domain that the PAS domain comprises a hydrophobic core that has no NMR-apparent a priori formed ligand cavity; detecting a first NMR spectrum of the PAS domain in the presence of the foreign ligand; comparing the first NMR spectrum with a second NMR spectrum of the PAS domain in the absence of the ligand; and therefrom determining the presence of the ligand specifically bound within the hydrophobic core of the PAS domain.