Patent ID: 7550159
Filing Date: 2009-06-23
Classification: A61K,A61P,C07K,C12P

Abstract:
1. A method to produce an orally ingestible composition of matter consisting essentially of mistletoe lectin I (ML-I), mistletoe lectin II (ML-II), and mistletoe lectin III (ML-III) essentially without nausea inducing compounds, the method comprising the following steps: i) pulverizing a start material consisting of pieces of Viscum album, ii) mixing the pulverized material with three to ten times the amount of 0.2 M acetic acid on w/w basis to form a solution and stirring said solution 12 to 24 hours at temperatures between 1 and 8° C., iii) filtering the solution through a coarse cloth, followed by filtering through a paper filter to remove turbidity and obtain a crude extract, iv) performing cationic exchange chromatography of the crude extract from (iii) on a cationic exchanger, v) washing the cationic exchanger with 0.15 M acetate buffer (pH 4.0) until absorption at 280 nm <0.5, vi) eluting ML-I, ML-II and ML-III collectively using a buffer containing 0.1 M Tris-HCl (pH 8.0) and 0.5 M NaCl and collecting fractions, vii) pooling fractions with absorbance >1.0 at 280 nm and dialyzing the pooled fractions overnight against 10 volumes of PBS (minus NaCl) to obtain a final extract, viii) aliquoting the final extract.