Patent ID: 6596497
Filing Date: 2003-07-22
Classification: A61K,C07K

Abstract:
A method for the screening of antiviral compounds comprising:(a) capturing polyclonal antibodies from an animal other than a mouse, directed against the HIV-1 gp41 trimeric structure containing three N-peptides of HIV-1 gp41 and three C-peptides of HIV-1 gp41, onto a solid-phase to form a polyclonal antibody coated solid-phase, (b) forming a mixture of a compound to be tested with N-peptides of HIV-1 gp41, and then adding C-peptides of HIV-1 gp41, (c) adding the mixture from step (b) to the polyclonal antibody coated solid-phase from step (a), then removing unbound peptides and unbound compound, and then adding a monoclonal antibody which specifically reacts with the HIV-1 gp41 trimeric structure containing three N-peptides of HIV-1 gp41 and three C-peptides of HIV-1 gp41, but does not react with individual N-peptides of HIV-1 gp41 and does not react with individual C-peptides of HIV-1 gp41, and (d) measuring the binding of said monoclonal antibody.