Patent ID: 8209129
Filing Date: 2012-06-26
Classification: G16B

Abstract:
1. A method to identify and produce a single copy sequence in a target reference complete genome sequence by successive division of the target reference genome sequence into subintervals and comparison of the subintervals to the target reference sequence, said method comprising: (A) determining a count of the number of times a subsequence of a first screened sequence occurs in the target reference genome sequence, said screened sequence being at least one subinterval of the target reference genome sequence obtained by division of the target reference genome sequence, wherein (B) determining a count of the number of times a subsequence of a second screened sequence occurs in the target reference genome sequence wherein said screened sequence is at least one subinterval of the target reference genome sequence, and wherein (C) identifying a single copy interval as a single copy sequence of the target reference sequence suitable for use as a single copy hybridization probe; and (D) producing the single copy sequence as a nucleic acid molecule, which is then hybridized to genomic DNA using at least one post-hybridization wash comprising a combination of low salt concentration and temperature exceeding the melting temperature of a divergent repetitive element forming a duplex with all cross-hybridizing sequences in the genome.