Patent ID: 8765462
Filing Date: 2014-07-01
Classification: A61K,A61P,C07K,C12N

Abstract:
1. A composition, vector construct or virus comprising: a lentiviral vector; wherein the lentiviral vector comprises a central polypurine tract (cPPT) sequence, optionally SEQ ID NO: 2 and/or a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), optionally SEQ ID NO: 3; a 5′-Long terminal repeat (LTR); HIV signal sequence, HIG Psi signal 5′-splice site (SD); delta-GAG element; Rev Responsive Element (RRE); 3′-splice site (SA); a CMV promoter; Elongation factor (EF) 1-alpha promoter and 3′-Self inactivating LTR (SIN-LTR); and a sequence corresponding to position 100 to position 3513 of SEQ ID NO: 18; and an IL-12 expression cassette, wherein the IL-12 expression cassette comprises a polynucleotide, optionally a polynucleotide encoding a p35 polypeptide and a p40 polypeptide; or a polynucleotide encoding an IL-12 fusion polypeptide, wherein the IL-12 fusion polypeptide has at least 90% sequence identity to an IL-12 fusion polypeptide encoded by SEQ ID NO:20 or 21 and the IL-12 fusion polypeptide activates an IL-12 receptor.