Patent ID: 7972827
Filing Date: 2011-07-05
Classification: B01J,B82Y,C07K,C12N,C40B,G01N,Y10S

Abstract:
1. A surface for immobilizing a polypeptide of interest to the surface, the surface comprising immobilized and modified DnaE C-intein polypeptide, wherein at least one functional amino acid side-chain of an amino acid residue selected from the group consisting of Asp17, Asp23, Asn25, Asn30, Asn36, Gln13 and Gln22 of the C-intein or at least one backbone amide group of an amino acid residue selected from the group consisting of Gly6, Gly11, Gly19, Gly31, Ala29, Ala32, Ala34, and Ala35 of the C-intein is protected using a 2-nitrobenzyl-based protecting group.