Patent ID: 6124120
Filing Date: 2000-09-26
Classification: C12Q

Abstract:
A method of amplifying a target nucleic acid sequence, the method comprising,(a) mixing a set of primers with a target sample, to produce a primer-target sample mixture, and incubating the primer-target sample mixture under conditions that promote hybridization between the primers and the target sequence in the primer-target sample mixture,(b) mixing DNA polymerase with the primer-target sample mixture, to produce a polymerase-target sample mixture, and incubating the polymerase-target sample mixture under conditions that promote replication of the target sequence,wherein the set of primers comprises a right set of primers and a left set of primers,wherein the target sequence is double-stranded, having a first and a second strand,wherein the right set primers are all complementary to the first strand of the target sequence and the left set primers are all complementary to the second strand of the target sequence,wherein the right set of primers has 4 or more primers and the left set of primers has 4 or more primers,wherein replication of the target sequence results in replicated strands, wherein during replication at least one of the replicated strands is displaced from the target sequence by strand displacement replication of another replicated strand.