Patent ID: 8241646
Filing Date: 2012-08-14
Classification: A61K,A61P,C07K

Abstract:
1. A method for generating in vitro an antibody, or one of its functional fragments directed against a tumoral antigen expressed at the surface of a tumor resistant to at least one anti-tumoral compound, the method comprising: (a) immunizing second animals directly with a milled homogenate and/or a suspension and/or a cell lysate stemming from said resistant tumor, wherein the immunized second animals produce antibodies to the tumoral antigen expressed at the surface of the tumor resistant to the anti-tumoral compound, and (b) screening antibodies produced by the immunized second animals for antibodies and functional fragments thereof that specifically recognize the tumoral antigen expressed at the surface of the resistant tumor and not expressed at the surface of the native tumor from which the resistant tumor was derived; wherein said resistant tumor is produced by grafting a tumor line and/or all or part of a human tumor on a first animal and then treating the first animal by administering the anti-tumoral compound for which it is desired to induce a resistance in the native tumor and said anti-tumoral compound comprises at least one monoclonal antibody.