Patent ID: 7955833
Filing Date: 2011-06-07
Classification: A61K,A61P,C12N,Y02A

Abstract:
1. A method of producing an immunogenic composition comprising a virus or a virus antigen, wherein the method comprises: a) providing a culture of VERO cells that have been grown in an animal protein free medium comprising soy hydrolysate at a concentration of about 0.05% (w/v) to about 1% (w/v) and yeast hydrolysate at a concentration of about 0.05% (w/v) to about 0.3% (w/v), wherein at least 90% of the hydrolysates have a molecular weight of less than or equal to 1000 Daltons; b) infecting the cells with the virus; c) incubating the infected cells to propagate the virus; d) harvesting the virus or virus antigen produced; and e) preparing an immunogenic composition from the harvested virus or virus antigen.