Patent ID: 7645599
Filing Date: 2010-01-12
Classification: C07D,C12N,C12P

Abstract:
1. A method for producing (R)-3-quinuclidinol hydrochloride having an optical purity of 93.4% ee or higher, the method comprising the steps of: (a) contacting a 3-quinuclidinone at a concentration of 5 to 50% with an isolated protein selected from the group consisting of the following (i) to (iii) in the presence of a reduced coenzyme to perform asymmetric reduction, a coenzyme-regenerating enzyme which regenerates said reduced coenzyme from its oxidized form, and a substrate for said coenzyme-regenerating enzyme; (b) recovering the (R)-3-quinuclidinol; (c) adjusting the pH of the (R)-3-quinuclidinol solution to alkaline levels to yield free (R)-3-quinuclidinol; (d) extracting the free (R)-3-quinuclidinol with n-butanol; (e) adding hydrochloric acid to the extract; (f) removing moisture from the extract; and (g) crystallizing (R)-3-quinuclidinol hydrochloride in the solution obtained in (f).