Patent ID: 8859224
Filing Date: 2014-10-14
Classification: C12M

Abstract:
1. A microinjection method applied to a microinjection device that includes an injecting member having a hollow point portion and introduces a substance contained in the injecting member to a solution including endoplasmic reticulum, the microinjection method comprising: measuring a correlation, for a first solution, between fluorescence intensity and injection amount, the measuring including: injecting the first solution into a third solution that does not form an interface with the first solution through the injecting member, and measuring pressure and pressurizing time; irradiating the third solution including the first solution with excitation light; detecting fluorescence intensity of the first solution included in the third solution; calculating an injection amount of the first solution injected into the third solution from the detected fluorescence intensity and the measured correlation; obtaining a correlation between an injection amount of the first solution from the injecting member, pressure, and pressurizing time based on the measured pressure and pressurizing time and the calculated injection amount; and adjusting an amount of the first solution injected into the third solution including the endoplasmic reticulum by controlling pressure and pressurizing time based on the obtained correlation between the injection amount, the pressure, and the pressurizing time.