Patent ID: 6080912
Filing Date: 2000-06-27
Classification: A01K,C07K,C12N

Abstract:
A method for introducing a polynucleotide contained within the genome of a recombinant retrovirus into the genome of a bovine unfertilized oocyte, comprising:a) providing:i) a bovine unfertilized egg comprising an oocyte having a plasma membrane and a zona pellucida, said plasma membrane and said zona pellucida defining a perivitelline space;ii) an aqueous solution comprising a polynucleotide contained within the genome of a recombinant retrovirus; andb) introducing said solution comprising said recombinant retrovirus into said perivitelline space under conditions which permit the integration of said polynucleotide contained within the genome of the recombinant retrovirus into the genome of said oocyte.