Patent ID: 8700381
Filing Date: 2014-04-15
Classification: G16B

Abstract:
1. Method for the relative quantification of at least one target nucleic acid in a sample comprising the steps: (a) amplifying each of the at least one target nucleic acids contained in the sample and concurrently obtaining signals correlated to the amplification of each of the at least one target nucleic acids, (b) amplifying each of at least one sample of comparative nucleic acids and concurrently obtaining signals correlated to the amplification of each of the at least one comparative nucleic acids, (c) correcting the signals obtained for background signals, (d) calculating a cycle-to-cycle amplification efficiency Ê(C) for each amplification cycle of target and comparative nucleic acids, (e) calculating a measure of randomness M of the cycle-to-cycle amplification efficiency Ê(C) for target and comparative nucleic acids, (f) identifying cycle numbers CM where M is minimal for target and comparative nucleic acids, (g) calculating characteristic cycle numbers CC from values of CM, (h) determining relative amounts of each of the at least one target nucleic acids in the sample in relation to the comparative nucleic acids by comparing CC of target and comparative samples.