Patent ID: 9146245
Filing Date: 2015-09-29
Classification: C07K,G01N

Abstract:
1. A method for selectively detecting vascular endothelial growth factor (VEGF) forms greater than 110 amino acids (VEGF 110+ ) in a biological sample, the forms including VEGF 121 , but not VEGF 110 , the method comprising the steps of: (a) contacting and incubating the biological sample with a capture reagent immobilized to a solid support to form an immobilized capture reagent-target molecule complex, wherein the capture reagent comprises a monoclonal antibody that specifically detects VEGF forms greater than 110 amino acids, including VEGF (b) separating the biological sample from the immobilized capture reagent-target molecule complex; (c) contacting the immobilized capture reagent-target molecule complex with a detectable antibody that binds to the KDR and/or FLT1 receptor binding domains of VEGF or that binds to an epitope within residues 1-110 of human VEGF; and (d) measuring the level of VEGF