Patent ID: 8741556
Filing Date: 2014-06-03
Classification: G01N,Y10T

Abstract:
1. A method for quantifying one or a plurality of first biomolecules in a human sample, comprising: (a) extracting a plurality of second biomolecules comprising one or a plurality of reference biomolecules from a mixture of at least two different human cell populations, wherein said at least two different human cell populations are populations of cells from different human cell lines; wherein said one or said plurality of reference biomolecules are a metabolically isotope labeled form of said one or said plurality of first biomolecules, thereby obtaining a standard mixture; (b) mixing said standard mixture with said human sample; (c) determining the quantity of said one or said plurality of first biomolecules in said human sample; and (d) comparing the quantity determined in step (c) to the quantity of one or a plurality of reference biomolecules amongst a plurality of second biomolecules in said standard mixture, wherein said first and reference biomolecules are proteins or peptides, and wherein the quantity of said one or said plurality of first biomolecules and said one or said plurality of reference biomolecules is determined by mass spectrometry.