Patent ID: 7425413
Filing Date: 2008-09-16
Classification: C12P

Abstract:
1. A method of creating a clinical reference solution that models clinically relevant sites on genes responsible for human genetic conditions, wherein the clinical reference solution is substantially free of clinically irrelevant nucleic acid, comprising: for each clinically relevant site, designing an oligonucleotide comprising an arrangement of bases to model the clinically relevant site as isolated from clinically irrelevant nucleic acid that occurs adjacent to the corresponding clinically relevant site in vivo, including designing two ends of the arrangement to form primer targets for differentially amplifying the modeled clinically relevant site; for each arrangement, performing a synthesis that includes constructing base-by-base, from end to end, a single strand of bases comprising the arrangement of bases that models the clinically relevant site and forms the primer targets associated with the clinically relevant site; and mixing each single strand into a single solution to form a collection of oligonucleotides, each representing a clinically relevant site of a gene.