Patent ID: 7005278
Filing Date: 2006-02-28
Classification: A61P,C12Q

Abstract:
1. A method for determining a 5-Fluorouracil-based chemotherapeutic regimen for treating a tumor in patient comprising: (a) obtaining a tumor sample from the patient; (b) fixing at least a portion of said tumor sample in paraffin to achieve a fixed and paraffin embedded (FPE) tumor tissue sample, (c) isolating mRNA from said FPE tumor tissue sample; (d) subjecting the mRNA isolated from said FPE tumor tissue to amplification using a pair of oligonucleotide primers SEQ ID NO: 1 and SEQ ID NO: 2 to obtain an amplified sample, (e) determining the amount of Dihydropyrimidine Dehydrogenase (DPD) mRNA in the amplified sample; (f) comparing the amount of Dihydropyrimidine Dehydrogenase (DPD) mRNA in the amplified sample with a predetermined threshold level for DPD expression; (g) determining a 5-Fluorouracil-based chemotherapeutic regimen for said patient based on the difference in amount of DPD mRNA in the amplified sample and the threshold level for DPD gene expression.