Patent ID: 6465447
Filing Date: 2002-10-15
Classification: A61K,A61P

Abstract:
A method for separating an active fraction and an active component from Tochukaso, said active fraction and said active component having antitumor activity, said method comprising:drying a fruit body of Tochukaso; grinding said dried fruit body to prepare a dried ground sample; extracting said sample with a water-miscible solvent to prepare an extracted solution; concentrating said extracted solution to prepare a crude extract; separating said crude extract at least once by silica gel column chromatography to obtain an eluted fraction having tumor cell proliferation inhibitory activity and to prepare the active fraction; and purifying said active fraction using chromatographic separation comprising thin layer chromatography or high performance liquid chromatography to separate the active component having tumor cell proliferation inhibitory activity, wherein the active component is a compound represented by formula (I): wherein said formula R is an acyl group represented by the formula â€”CO(CH2)nCH3 in which n is an integer from 0 to 5.