Patent ID: 6015681
Filing Date: 2000-01-18
Classification: G01N,Y10S

Abstract:
A method for semiquantitatively detecting, in less than 30 minutes, a predesignated, target cariogenic bacteria in a sample selected from the group consisting of human dental plaque, saliva, and oral rinse which comprises the following steps:(a) treating the sample with a antibody stripping buffer that removes all host antibodies from any target cariogenic bacteria present in the sample and said buffer does not support an antigen/antibody interaction;(b) spotting onto and filtering the stripping buffer treated bacteria suspension or solution through a blocked solid phase substrate thereby retaining the treated target bacteria in a spot on the blocked solid phase substrate;(c) reacting the retained treated target bacteria spot with a primary antibody specific for the target cariogenic bacteria;(d) previously, simultaneously or subsequently to said primary antibody reacting with the target cariogenic bacteria reacting the primary antibody with a conjugated label producing a detectable signal; and(e) detecting the signal whereby the presence of the target bacteria is determined in the sample by the intensity of the signal.