Patent ID: 7820440
Filing Date: 2010-10-26
Classification: A61K,A61P,C12N

Abstract:
1. A method for producing a recombinant adenovirus comprising a recombinant adenovirus genome comprising sequences encoding structural and non-structural elements of an adenovirus of a first serotype and sequences encoding an E4orf6 protein from an adenovirus of a second serotype wherein the first serotype is different from the second serotype, said method comprising the steps of: providing a complementing cell comprising an E1B-55K-encoding sequence from the adenovirus of the second serotype wherein the E1B-55K-encoding sequence is operably linked to expression control sequences, introducing into the complementing cell the recombinant genome wherein the sequence encoding E4orf6 from the adenovirus of the second serotype is operably linked to an expression control sequence, and wherein the recombinant genome does not comprise a sequence encoding a functional E4-orf6 protein from the adenovirus of said first serotype; culturing said complementing cell wherein the step of introducing comprises the cell in a medium under conditions allowing for production and assembly of the recombinant adenovirus to take place; and harvesting the recombinant adenovirus so produced from the medium and/or the complementing cell.