Patent ID: 7723094
Filing Date: 2010-05-25
Classification: A61K,A61P,C12N,Y02A

Abstract:
1. A composition comprising a plurality of vectors, comprising a) vectors for vRNA production including a vector comprising a promoter operably linked to an influenza virus PA cDNA linked to a transcription termination sequence, a vector comprising a promoter operably linked to an influenza virus PB1 cDNA linked to a transcription termination sequence, a vector comprising a promoter operably linked to an influenza virus PB2 cDNA linked to a transcription termination sequence, a vector comprising a promoter operably linked to an influenza virus HA cDNA linked to a transcription termination sequence, a vector comprising a promoter operably linked to an influenza virus NP cDNA linked to a transcription termination sequence, a vector comprising a promoter operably linked to an influenza virus NA cDNA linked to a transcription termination sequence, a vector comprising a promoter operably linked to an influenza virus M cDNA linked to a transcription termination sequence, and a vector comprising a promoter operably linked to an influenza virus NS cDNA linked to a transcription termination sequence, wherein at least one vector comprises a RNA polymerase II promoter 5′ to a first ribozyme sequence which is 5′ to the viral cDNA which is 5′ to a second ribozyme sequence which is 5′ to the transcription termination sequence, wherein any vector for vRNA production which does not have a RNA polymerase II promoter, has a RNA polymerase I promoter; and b) vectors for mRNA production including a vector comprising a promoter operably linked to a DNA segment encoding influenza virus PA, a vector comprising a promoter operably linked to a DNA segment encoding influenza virus PB1, a vector comprising a promoter operably linked to a DNA segment encoding influenza virus PB2, or a vector comprising a promoter operably linked to a DNA segment encoding influenza virus NP; and wherein transcription from the RNA polymerase II promoter of the at least one vector comprising the RNA polymerase II promoter 5′ to a first ribozyme sequence which is 5′ to the viral cDNA which is 5′ to a second ribozyme sequence which is 5′ to the transcription termination sequence, results in a transcript that is a template for influenza virus polymerase, and wherein transfection of a vertebrate cell with the plurality of vectors yields infectious influenza virus.