Patent ID: 6528290
Filing Date: 2003-03-04
Classification: C12N,C12P,C12R,Y10S

Abstract:
A fermentation process for manufacturing mannitol with high yield and high productivity, using strain of Candida magnoliae (KCCM-10252) comprising the steps of:i) fermenting glucose or fructose medium with microorganism by controlling following fermentation conditions; a) composition of medium for maximum production of mannitol consists of 50Ëœ100 g/L of glucose, 50Ëœ120 g/L of fructose, 5Ëœ20 g/L of yeast extract 1Ëœ5 g/L of ammonium sulfate, 1.0Ëœ5.0 g/L of dibasic potassium phosphate and 0.1Ëœ0.5 g/L of magnesium sulfate; b) pH of culture medium is 4.5Ëœ5.5; c) temperature of cultivation is 27Ëœ35Â° C.; d) aeration rate is 0Ëœ0.5 volume of air per volume of medium per minute; and e) agitation speed of the medium is 200Ëœ500 rpm; ii) removing the cells and other residue from the fermentation medium; and iii) separating and recovering mannitol from the fermentation medium of step ii).