Patent ID: 6818421
Filing Date: 2004-11-16
Classification: C07H,C12N

Abstract:
A method of producing linear &agr;-1,4 glucans comprising using a protein having the enzymatic activity of an amylosucrase that is coded for by a DNA molecule comprising a first DNA sequence encoding said protein, wherein said first DNA sequence has more than 60% sequence identity to a second DNA sequence selected from the group consisting of:(a) a DNA sequence coding for a protein comprising SEQ ID NO:2; (b) the coding region of SEQ ID NO:1; (c) a DNA sequence encoding a protein having amylosucrase activity in the DNA insert of plasmid pNB2 (Deutsche Sammlung von Mikroorganismen (DSM) 9196); (d) a DNA sequence coding for a protein encoded by the DNA insert of plasmid pNB2 (DSM 9196); (e) a part of any one of the DNA sequences of (a)-(d) coding for a protein having the enzymatic activity of an amylosucrase; and (f) a full length complement of the DNA sequence of any one of (a)-(e); incubating said protein encoded by said first DNA sequence with sucrose under conditions that allow said protein to produce linear &agr;-1,4 glucans; and isolating the linear &agr;-1,4 glucans.