Patent ID: 8697047
Filing Date: 2014-04-15
Classification: A61K,A61P

Abstract:
1. A stable immunogenic product for inducing antibodies risen against IFNα protein in a subject comprising protein immunogenic heterocomplexes consisting of associations between (i) IFNα molecules and (ii)KLH protein carrier molecules wherein more than 1% and less than 40% of the IFNα molecules are covalently linked to the KLH protein carrier molecules (ii), and more than 60% of IFNα molecules are non-covalently associated with the protein carrier molecules (ii), and wherein said stable immunogenic product is produced by a process comprising the following steps: a. incubating the IFNα proteins and KLH protein carrier molecules in a molar ratio IFNα:KLH ranging from 10:1 to 50:1 in the presence of glutaraldehyde to produce immunogenic heterocomplexes; b. removing excess glutaraldehyde; c. stabilizing the immunogenic heterocomplexes with formaldehyde; d. adding glycine to block the reaction with formaldehyde in step c); e. performing a dialysis of the product obtained at step d); and f. collecting the product comprising immunogenic heterocomplexes prepared at step e).