Patent ID: 9035031
Filing Date: 2015-05-19
Classification: A23C,A23J,A23L,A23V,C07K

Abstract:
1. A method for providing a β-lactoglobulin product and an α-enriched whey protein isolate from whey, the method comprises the steps of: (i) providing whey obtained from an animal, (ii) adjusting the pH value of the whey to pH 4.5 or below, (iii) loading the whey to an expanded bed adsorption chromatographic support, said chromatographic support comprising an expanded bed adsorption chromatography adsorbent, said expanded bed adsorption chromatography adsorbent comprising sulfonic acid ligands in a concentration of at least 50 mmol/L adsorbent, (iv) optionally washing the expanded bed adsorption chromatographic support using a wash buffer and obtaining a wash fraction, (v) eluting the β-lactoglobulin product from the expanded bed adsorption chromatographic support using a first elution buffer, said first elution buffer having a pH of 5 or less and a salt concentration of at least 0.1M and (vi) eluting the α-enriched whey protein isolate with a second elution buffer.