Patent ID: 7666581
Filing Date: 2010-02-23
Classification: A61K,A61P

Abstract:
1. A method of preparing complexes of heat shock protein and antigenic peptides, said method comprising the step of complexing a population of antigenic peptides to one or more different heat shock proteins in vitro; wherein the population of antigenic peptides contains at least 50 different peptides and, prior to said complexing step, is produced by a method comprising aliquoting a protein preparation into a plurality of reaction mixtures, wherein each reaction mixture is subjected to (i) digestion with a different protease, or (ii) a different non-enzymatic cleavage method; and pooling the digested or non-enzymatically cleaved reaction mixtures; wherein said protein preparation (a) is from antigenic cells or a cellular fraction thereof, and (b) comprises at least 50% of the different proteins or at least 50 different proteins of said antigenic cells or said cellular fraction.