Patent ID: 6884607
Filing Date: 2005-04-26
Classification: C12N,C12P

Abstract:
1. An isolated polynucleotide sequence containing: a) a polynucleotide sequence coding for an amino acid sequence represented by SEQ ID NO: 1; b) a polynucleotide sequence coding for an enzyme capable of preferentially producing (S)-4-bromo-3-hydroxybutanoate by asymmetrically reducing 4-bromo-3-oxobutanotate, wherein said polynucleotide sequence hybridizes, under high ion concentrations of 900 mM sodium chloride and 90 mM sodium citrate at 65° C. and the hybrid formed is maintained as a hybrid after being kept at 65° C. for 30 minutes under a low ion concentration of 15 mM sodium chloride and 1.5 mM sodium citrate, to a polynucleotide sequence encoding SEQ ID NO: 1; c) a polynucleotide sequence represented by SEQ ID NO: 2; d) a polynucleotide sequence coding for an amino acid sequence comprising SEQ ID NO: 1 having additional 6 amino acids of Trp-Ile-Ser-Thr-Lys-Leu SEQ ID NO: 37 at the C-terminal of the amino acid sequence; or e) a polynucleotide sequence coding for an an enzyme capable of preferentially producing (S)-4-bromo-3-hydroxybutanoate by asymmetrically reducing 4-bromo-3-oxobutanoate, wherein said polynucleotide sequence hybridizes, under high ion concentrations of 450 to 900 mM sodium chloride and 45 to 90 mM sodium citrate at 65° C. and the hybrid formed is maintained as a hybrid after being kept at 65° C. for 30 minutes under a low ion concentration of 15 to 300 mM sodium chloride and 1.5 to 30 mM sodium citrate and 0.1 to 1.0 wt % of SDS, to a polynucleotide sequence encoding SEQ ID NO:1.