Patent ID: 6498011
Filing Date: 2002-12-24
Classification: C07K,C12N

Abstract:
A method for introducing nucleic acids into mammalian cells, comprising:(a) preparing a chimeric recombinant nucleic acid construct that comprises in operable linkage to an expressible DNA or to an oligonucleotide at least one T-DNA border sequence or functional part thereof, which serves as a substrate in the VirD2 cleavage reaction; (b) cleaving of the nucleic acid substrate prepared according to step (a) by means of VirD2 protein, wherein the cleavage of the substrate by means of VirD2 is carried out in the presence of wild-type VirE2; and (c) introducing the cleaved nucleic acid comprising the VirD2 protein covalently bound to the 5â€² end of the VirD2 cleavage site and wild-type VirE2 protein into the cells.