Patent ID: 8592141
Filing Date: 2013-11-26
Classification: G01N

Abstract:
1. A method of detecting a component of interest in a mixture of components, the method comprising: (i) flowing the mixture of components through a binding channel region, which binding channel region comprises a component-binding moiety, thereby binding at least a portion of the component of interest to the component-binding moiety; (ii) flowing the mixture of components through a separation channel region, resulting in one or more separated components, wherein the separation channel region comprises a separation buffer, which buffer comprises a detergent; (iii) mixing a first portion of diluent with the one or more separated components, thereby diluting the detergent; (iv) detecting the one or more separated components; (v) releasing the component of interest from the component-binding moiety, resulting in a released component of interest; (vi) flowing the released component of interest through the separation channel region; (vii) mixing a second portion of diluent with the released component of interest, thereby diluting the detergent; and (viii) detecting the released component of interest.