Patent ID: 8889378
Filing Date: 2014-11-18
Classification: C12Q,G01N

Abstract:
1. A method for identifying a compound that inhibits cytochrome c synthesis in a bacterial cell, the method comprising: a. contacting a transfected bacterial cell with a compound, the bacterial cell being transfected with a first expression vector encoding one or more Ccs protein selected from the group consisting of (i) CcsA and CcsB, and (ii) a fusion of CcsA and CcsB, and a second expression vector encoding a cytochrome c reporter protein, wherein the bacterial cell comprises a chromosomal mutation such that expression of a nucleic acid sequence encoding one or more Ccm protein is reduced and the bacterial cell cannot synthesize cytochrome c; and b. determining the amount of cytochrome c reporter protein produced in the presence of the compound relative to the amount produced in the absence of the compound, wherein a decrease in amount is an indication that the compound inhibits the synthesis of cytochrome c.