Patent ID: 6184344
Filing Date: 2001-02-06
Classification: C07K

Abstract:
A method for ligating a first oligopeptide with a second oligopeptide end to end for producing an oligopeptide product, the method comprising the following steps:Step A: admixing the first and second oligopeptides in a reaction solution including a catalytic thiol, the first oligopeptide including a C-terminal thioester, the second oligopeptide including an N-terminal cysteine having an unoxidized sulfhydryl side chain; thenStep B: condensing the unoxidized sulfhydryl side chain of the N-terminal cysteine with the C-terminal thioester for producing an intermediate oligopeptide linking the first and second oligopeptides with a .beta.-aminothioester bond; and thenStep C: rearranging the .beta.-aminothioester bond of the intermedite oligopeptide of said Step B for producing the oligopeptide product linking the first and second oligopeptides with an amide bond.