Patent ID: 7666436
Filing Date: 2010-02-23
Classification: A61K,C07K

Abstract:
1. Bordetella pertussis, Bordetella parapertussis , or Bordetella bronchiseptica strains characterized in that they contain in their chromosome the gene coding for pertussis toxin (PT) and its expression and secretion regulation sequences wherein the nucleotide sequence of the S1 subunit of said gene is mutagenized so as to code for a pertussis toxin of no or reduced toxicity by having said nucleotide sequence coding for the amino acid Glu at PT position 129 changed to a sequence coding for the amino acid Gly, and by having a further change selected from the group consisting of: (1) said sequence coding for the amino acid Arg at PT position 9 changed to a sequence coding for Lys, (2) said sequence coding for the amino acid Arg at PT position 13 changed to a sequence coding for Leu, and (3) said sequence coding for the amino acid Trp at PT position 26 changed to a sequence coding for Ile.