Patent ID: 8986959
Filing Date: 2015-03-24
Classification: C12Q

Abstract:
1. A method for detecting a nucleic acid molecule within a hydrogel post array comprising a) Depositing on a substantially planar surface plural aqueous solutions each containing at least one nucleic acid capable of initiating an amplification of a selected template nucleic acid and a viscosity increasing solute, said plural aqueous solutions not being in fluid communication with each other and forming a multiplicity of deposits; b) Allowing said aqueous solutions to evaporate in said deposits; c) Establishing an array of hydrogel posts, each hydrogel post containing a cell-free, enzymatic, nucleic-acid amplification system, said hydrogel posts comprising the array being arranged so as any given post impinges upon only one deposit; d) distributing on at least one of said hydrogel posts nucleic acid molecules, at least one of which comprises a template nucleic acid for said amplification system; e) incubating said hydrogel posts under conditions promoting the synthesis of an amplified nucleic acid product by said amplification system from said at least one template; wherein said amplification system comprises at least two non-immobilized nucleic acids capable of promoting synthesis of amplified nucleic acid product from said template.