Patent ID: 6514739
Filing Date: 2003-02-04
Classification: C12N,C12Y

Abstract:
An isolated lysophospholipase comprising:a) a polypeptide encoded by a lysophospholipase encoding part of the DNA sequence cloned into a plasmid present in Escherichia coli deposit number DSM 13004; b) a polypeptide having an amino acid sequence of amino acids 1-600 in SEQ ID NO: 4; c) an analogue of the polypeptide defined in (a) or (b) which has at least 95% sequence homology with said polypeptide; or d) a polypeptide which is encoded by a nucleic acid sequence which hybridizes with a complementary strand of the nucleic acid sequence shown as nucleotides 115-1914 of SEQ ID NO: 3 under hybridization conditions comprising prehybridizing in a solution of 5Ã—SSC, 5Ã—Denhardt's solution, 0.5% SDS and 100 &mgr;g/ml of denatured sonicated salmon sperm DNA, followed by hybridization in the same solution for 12 hours at approx. 45Â° C., followed by washing in 2Ã—SSC, 0.5% SDS for 30 minutes at a temperature of at least 65Â° C.