Patent ID: 9012409
Filing Date: 2015-04-21
Classification: A61K,A61P,C07K

Abstract:
1. A method of costimulation, the method comprising contacting a T cell with a T cell activating stimulus and a costimulatory protein, wherein the costimulatory protein is selected from the group consisting of: (i) a polypeptide encoded by a nucleic acid sequence that encodes an amino acid sequence comprising an extracellular region of a B7-H5 polypeptide, wherein the nucleic acid sequence hybridizes over its full length, after a wash at 50° C. to 60° C. in a buffer containing 0.2×SSC and 0.1% SDS, to the complement of a nucleotide sequence consisting of nucleotide 88 to nucleotide 936 of SEQ ID NO:2; (ii) a polypeptide comprising all or part of amino acid 30 to amino acid 311 of SEQ ID NO:1 and including at least an V-like Ig domain consisting of amino acid 47 to amino acid 150 of SEQ ID NO:1; (iii) a polypeptide comprising all or part of amino acid 30 to amino acid 311 of SEQ ID NO:1 and including amino acid residues 40 to amino acid 190 of SEQ ID NO:1; (iv) a polypeptide comprising all or part of amino acid 30 to amino acid 311 of SEQ ID NO:1 and including amino acid residues 35 to amino acid 190 of SEQ ID NO:1; (v) a fusion protein comprising a first domain and at least one additional domain, wherein the first domain comprises a first polypeptide encoded by a nucleic acid sequence that encodes an amino acid sequence comprising an extracellular region of a B7-H5 polypeptide, wherein the nucleic acid sequence hybridizes over its full length, after a wash at 50° C. to 60° C. in a buffer containing 0.2×SSC and 0.1% SDS, to the complement of a nucleotide sequence consisting of nucleotide 88 to nucleotide 936 of SEQ ID NO:2 and the at least one additional domain comprises a second polypeptide that is unrelated to SEQ ID NO:1; (vi) a fusion protein comprising a first domain and at least one additional domain, wherein the first domain comprises a polypeptide encoded by a nucleic acid sequence that encodes an amino acid sequence comprising an extracellular region of a B7-H5 polypeptide, wherein the nucleic acid sequence hybridizes over its full length, after a wash at 50° C. to 60° C. in a buffer containing 0.2×SSC and 0.1% SDS, to the complement of a nucleotide sequence consisting of nucleotide 88 to nucleotide 936 of SEQ ID NO:2; and (vii) a polypeptide encoded by nucleic acid sequence which hybridizes over its full length, after a wash at 50° C. to 60° C. in a buffer containing 0.2×SSC and 0.1% SDS, to the complement of a nucleotide sequence consisting of nucleotide 88 to nucleotide 570 of SEQ ID NO:2.