Patent ID: 8334101
Filing Date: 2012-12-18
Classification: A61K,A61P,C12N,G01N

Abstract:
1. A method of identifying a compound that modulates an AIM2 polypeptide-mediated immune response, the method comprising: (a) providing a cell that expresses an exogenous AIM2 polypeptide comprising a pyrin (PYD) domain; (b) contacting the cell with a test compound to generate a test sample; (c) incubating the test sample under conditions and for a time sufficient such that the AIM2 polypeptide and an apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC polypeptide) would co-localize intracellularly if a reference sample lacking the test compound were incubated under said conditions and for said time; and (d) detecting a level of intracellular co-localization of the AIM2 polypeptide and the ASC polypeptide; wherein a change in the level of intracellular co-localization of the AIM2 polypeptide and the ASC polypeptide in the test sample compared to said reference sample indicates that the test compound is a compound that modulates the AIM2 polypeptide-mediated immune response.