Patent ID: 6492116
Filing Date: 2002-12-10
Classification: G01N

Abstract:
A method for identifying a substance that inhibits the binding of a dm2 protein to p53, while does not inhibit binding of p53 to DNA, the method comprising the steps of:(1) combining (a) a first protein which comprises a p53 DNA-binding domain and a p53 oligomerisation domain and wnich is able to bind to drm2, (b) a second protein which comprises a p53 binding domain from dm2, (c) a DNA fragment comprising a p53 binding domain, and (d) the substance to be tested; (2) discriminating between (i) the formation of a double complex of the first protein and the DNA fragment, which indicates that the substance has the ability to inhibit binding of a dm2 protein to p53, while not inhibiting binding of p53 to DNA, (ii) the formation of a triple complex of the first protein, the second protein and the DNA fragment, which indicates that the substance does not have the ability to inhibit binding of a dm2 protein to p53, and (iii) the non-formation of a complex between the first protein and the DNA fragment, which indicates that the substance inhibits the binding of p53 to DNA; and (3) identifying a substance which allows the formation of the double complex of (i).