Patent ID: 8053200
Filing Date: 2011-11-08
Classification: G01N

Abstract:
1. A method of testing a sample, comprising the steps of: combining a monocyte-containing reagent and a sample to be tested in a first assay system which includes at least one microtiter well shaped such that the monocyte-containing reagent is concentrated to provide greater cell to cell contact as compared to a flat-bottomed microtiter well, wherein the surface of the microtiter well comprises a polypropylene coating or the entire microtiter well is composed of polypropylene; incubating the monocyte-containing reagent and the sample; transferring the contents of the first assay system to a second assay system which comprises at least one surface treated with an antibody to the cytokine or endogenous mediator of the inflammatory response; and assaying the second assay system for the presence of cytokine or endogenous mediator bound to the antibody on the surface, whereby the sample is tested.