Patent ID: 9068215
Filing Date: 2015-06-30
Classification: C12M,C12Q

Abstract:
1. A method of homogenously mixing the contents of a plurality of bioreactors comprising: (a) providing a plurality of bioreactors, 1 through nth, in a series, installed on a same plane either in a series or in a circular pattern, the bioreactors comprising a bioreactor container with at least two surfaces and two opposing sides, to hold a liquid further comprising a nutrient media and a biological culture, a means of gassing the liquid further comprising a gas inlet, a gas sterilizing filter and a gas sparging filter, a gas exhaust, at least one nutrient media inlet/outlet, at least one liquid ports installed on the bottom with means of closing or opening the liquid port, a means of agitating the nutrient media, a plurality of sensors installed in the bioreactor container with at least a sensor for measuring the weight of the bioreactor container; (b) providing a receiving container capable of holding an appropriate quantity of the liquid wherein the receiving container further comprises a support capable of being raised or lowered; (c) connecting the liquid port of each bioreactor to the receiving container; (d) operating the bioreactors; (e) opening all liquid ports; (f) lowering the receiving container support to a position below the position of bioreactors; (g) measuring the increase in the weight of the receiving bioreactor to a pre-determined level; (h) raising the receiving container support to a position higher than the bioreactors; (i) measuring the decrease in the weight of the receiving container; (j) repeating the steps (f) to (i) periodically or continuously.