Patent ID: 7413855
Filing Date: 2008-08-19
Classification: C01D,C07H,C12Q

Abstract:
1. Method for the conversion of a cytosine base in a nucleic acid to an uracil base comprising a) incubating a solution comprising the nucleic acid for a time period that is not less than 1.5 hours and further is not more than 3.5 hours at a temperature between 70 and 90° C., wherein the concentration of bisulfite in the solution is between 3 M and 6.25 M and wherein the pH value of the solution is between 5.25 and 5.75, whereby the nucleic acid is deaminated, and b) incubating the solution comprising the deaminated nucleic acid under alkaline conditions whereby the deaminated nucleic acid is desulfonated.