Patent ID: 6051416
Filing Date: 2000-04-18
Classification: A61K,A61P,C12N,C12Q,C12R,G01N,Y02A,Y10S

Abstract:
A method of producing Helicobacter bacteria having enhanced antigenic properties which comprises growing a culture of the Helicobacter bacteria in vitro in a combination of conditions comprising:a) about 0.05% to about 3% bile or about 0.025% to about 0.6% of one or more bile acids or salts thereof;b) at a temperature between about 30.degree. C. and about 42.degree. C.;c) in air or an microaerophillic condition, wherein the microaerophillic condition comprises i) about 5% to about 20% CO.sub.2 with about 80% to about 95% air; or ii) about 5% to about 10% O.sub.2 with about 10% to about 20% CO.sub.2 with about 70% to about 85% N.sub.2 ; andd) a divalent cation chelator selected from the group consisting of 0 to about 100 .mu.M of BAPTA/AM, 0 to about 10 mM of EGTA, and 0 to about 100 .mu.M of EGTA/AM,for a sufficient time so that the culture is in a growth phase at about early log phase, between early log phase and stationary phase, or at about stationary phase.