Patent ID: 6916920
Filing Date: 2005-07-12
Classification: C07B,C07H

Abstract:
1. A method for determining a position of binding of a monosaccharide at a reducing end to a neighboring monosaccharide, the method comprising at least: (a) reacting a saccharide having a reducing end with a hydrazine of formula (I) to produce a hydrazone: wherein R 1 is a group other than hydrogen that has a detectable label and/or an immobilization support as its portion or can bind to a detectable label and/or an immobilization support; the bond between R 1 and N is a bond that is cleavable by a reaction that can cleave a glycosidic linkage; and R 2 is hydrogen or an alkyl group containing 1-8 carbon atoms; (b) reducing the hydrazone obtained in step (a) to a hydrazino derivative; (c) N-acetylating the hydrazino derivative obtained in step (b); (d) methylating a hydroxy group of the hydrazino derivative obtained in step (c); (e) cleaving the hydrazino derivative obtained in step (d) by the reaction that can cleave a glycosidic linkage to obtain a hydrazino monosaccharide derivative; (f) completely acetylating the hydrazino monosaccharide derivative obtained in step (e); and (g) identifying the acetylated hydroxyl group in the completely acetylated hydrazino monosaccharide derivative obtained in step (d), and determining a position of binding of a monosaccharide at a reducing end to a neighboring monosaccharide.