Patent ID: 8420324
Filing Date: 2013-04-16
Classification: C12N,C12P,C12Q

Abstract:
1. A method for reverse transcription of one or more nucleic acid molecules comprising contacting a solution comprising one or more nucleic acid templates with a reagent mixture, wherein said reagent mixture comprises a ready to use reagent solution that demonstrates prolonged stability when stored at −20° and that was stored at −20° C. for at least two weeks prior to contacting said template solution comprising one or more nucleic acid templates with said reagent mixture, wherein said ready to use reagent mixture comprises: (a) glycerol, and (b) a viral reverse transcriptase in a concentration sufficient for use in a reverse transcription reaction without adding additional reverse transcriptase, wherein said reverse transcriptase is selected from the group consisting of AMV RT, RSV RT, MMLV RT, HIV RT, EIAV RT, RAV2 RT, THERMOSCRIPT RT, ASLV and Rnase H (c) a buffer suitable for use in a reverse transcription reaction, wherein said buffer further comprises and incubating the resulting solution at a temperature suitable for cDNA synthesis.