Patent ID: 6333155
Filing Date: 2001-12-25
Classification: C12Q

Abstract:
A method of comparing the effects of a drug to the effects of a mutation, comprising:contacting a drug with a first population of cells;preparing a transcription indicator from the first population of cells, wherein a transcription indicator is selected from the group consisting of cellular RNA, cellular mRNA, cRNA and cDNA;preparing a transcription indicator from a second population of cells which population is not contacted with a drug, wherein the second population of cells carry a mutation in a gene of interest relative to the first population of cells;preparing a transcription indicator from a third population of cells which is not contacted with a drug and which does not carry the mutation;hybridizing the transcription indicators to oligonucleotide arrays to form a pattern of hybridization for each of said populations of cells;comparing each of the first and the second populations' patterns of hybridization to the third population's pattern of hybridization to identify changes caused by the drug and the mutation;comparing the changes caused by the drug to those caused by the mutation;identifying the gene of interest as a candidate target of the drug if the drug and the mutation affect hybridization to one or more common oligonucleotides; identifying the drug as affecting targets other than the gene if the drug affects hybridization to both common oligonucleotides and unique oligonucleotides.