Patent ID: 8734782
Filing Date: 2014-05-27
Classification: A01K,C12N,C12P

Abstract:
1. A recombinant host cell for the production of a compound of interest, said host cell comprising at least two substantially homologous DNA domains comprising flanking regions of at least 100 base pairs and overall homology of the flanking regions of at least 60%, wherein said at least two substantially homologous DNA domains are suitable for integration of one or more copies of a polynucleotide of interest wherein at least one of the at least two substantially homologous DNA domains is an adapted domain that has enhanced integration preference for the polynucleotide of interest compared to a substantially homologous DNA domain said adapted domain originates from, wherein the substantially homologous DNA domain where the adapted substantially homologous DNA domain originates from has a gene conversion frequency that is at least 10% higher than another of the at least two substantially homologous DNA domains, and wherein the at least two substantially homologous DNA domains are loci of fungal glucoamylase or are loci of fungal amyB.