Patent ID: 6464972
Filing Date: 2002-10-15
Classification: A61K,A61P,C12N,Y02A

Abstract:
A recombinant poliovirus constructed from a poliovirus having a 5â€²NTR containing an internal ribosomal entry site (IRES), and the coding sequences for structural proteins (P1), and for the non-structural proteins (P2 and P3) and a 3â€²NTR selected from the group consisting of wild type serotype 1, serotype 2, and serotype 3, whereina. i. a part of the IRES, at least 19 nucleotides, of the poliovirus is substituted with a part of the IRES, at least 19 nucleotides, of Human Rhinovirus also having a 5â€²NTR region containing an internal ribosomal entry site (IRES), the coding sequences of structural proteins (P1) and for the non-structural proteins (P2 and P3) and a 3â€²NTR, or â€ƒii. a part of the IRES, at least 19 nucleotides, of the poliovirus is substituted with a part of the IRES, at least 19 nucleotides, of a virus selected from the group of picornaviruses comprising Human Rhinovirus serotype 1, 3-100, coxsackievirus serotype B1-B6, human echovirus serotype 1-7, 11-27, and 29-33, also having a 5â€²NTR region containing an internal ribosomal entry site (IRES), the coding sequences of structural proteins (P1), and for the non-structural proteins (P2 and P3) and 3â€²NTR, and wherein b. optionally, P1 of the poliovirus is substituted with P1 of a Poliovirus (Sabin), selected from the groups consisting of PV1(S), PV2(S) and PV3(S); c. optionally, at least 3Dpol of P3 of the poliovirus is substituted with at least 3Dpol of P3 of a Poliovirus (Sabin), selected from the groups consisting of PV1(S), PV2(S) and PV3(S); and d. optionally, 3â€²NTR of the poliovirus is substituted with the 3â€²NTR of a Poliovirus (Sabin), selected from the group consisting of PV1(S), PV2(S), PV3(S).