Patent ID: 7851154
Filing Date: 2010-12-14
Classification: C12Q

Abstract:
1. A method for determining the degree of methylation of genomic DNA, said method comprising: (a) providing a genomic DNA; (b) modifying said DNA by bisulfate conversion of cytosine to uracil; (c) amplifying said bisulfite-modified DNA by PCR to produce a first PCR product; (d) amplifying said first PCR product by PCR using a tag-modified primer to yield a second PCR product comprising a cytosine/guanosine enriched tag, wherein said tag-modified primer comprises a region that is complementary to a target DNA and a tag having a non-complementary nucleotide sequence containing at least 50% cytosine or guanosine; (e) purifying said second PCR product; (f) sequencing said purified second PCR product comprising the cytosine/guanosine enriched tag to obtain a nucleotide sequence of said second PCR product; (g) analyzing the nucleotide sequence obtained from step (f) to determine the degree of methylation of said DNA.