Patent ID: 6436705
Filing Date: 2002-08-20
Classification: A01N

Abstract:
A shape stabilized erythrocyte obtained by a method consisting of:treating erythrocyte with CO gas; reacting said erythrocyte with a reversible cross-linking agent in the presence of a high concentration of a low molecular-weight cryoprotectant; and lyophilized said erythrocyte; wherein said reversible cross-linking agent is selected from the group consisting of diamide and dimethyl-3,3â€²-dithiobispropionimidate (DTBP), wherein said crosslinking-agent is reacted at a concentration of from about 5 mM to about 10 mM from about 1 hour to about 5 hours, wherein said erythrocyte is suspended in a loading medium, incubated and resuspended in a lyophilization medium prior to said lyophilization, wherein said low molecular weight cryoprotectant is selected from the group consisting of glucose, fructose, sucrose, trehalose, raffinose and combinations thereof, and wherein said high concentration of said low molecular weight cryoprotectant is of from about 500 mM to about 800 mM.