Patent ID: 6538113
Filing Date: 2003-03-25
Classification: C07K,C12N,C12Y,G01N,Y10S

Abstract:
A method of obtaining an antibody that specifically binds prothrombin fragments F2/F1.2 and that does not bind intact prothrombin, comprising immunizing an animal with an immunizing peptide that is at least four and less than eight amino acids in length,wherein the carboxyl-terminus of the immunizing peptide ends with the amino acids Ile Glu-Gly-Arg-OH, and wherein said animal produces antibodies after such immunizing, and purifying the antibody that specifically binds prothrombin fragments F2/F1+2 and that does not bind intact prothrombin by immunoadsorbing the antibody to a carrier-bound immunoadsorbing peptide that is at least four and less than eight amino acids in length and that includes the amino acid sequence lle-Glu-Gly-Arg-OH, and recovering the antibody from the carrier bound immunoadsorbing peptide.