Patent ID: 7361496
Filing Date: 2008-04-22
Classification: A61K,C07K,C12N

Abstract:
1. A method for producing a recombinant mumps virus comprising; in at least one host cell, conducting transfection or transformation, in media, of a rescue composition which comprises (i) a transcription vector comprising an isolated nucleic acid molecule which comprises a polynucleotide sequence encoding an antigenome of mumps virus, or variant polynucleotide sequence thereof, and (ii) at least one expression vector which comprises one more isolated nucleic acid molecule(s) comprising a polynucleotide sequence encoding the trans-acting proteins (NP, P and L) necessary for encapsidation, transcription and replication; under conditions sufficient to permit the co-expression of said vectors and the production of the recombinant virus; wherein the isolated nucleic acid molecule encoding an antigenome of mumps virus comprises the polynucleotide sequence selected from the group consisting of SEQ. ID NOS. 1, 11 and 12; and wherein the recombinant virus is harvested.