Patent ID: 6365713
Filing Date: 2002-04-02
Classification: A61K,C07K

Abstract:
A substantially purified protein which inhibits viral infection of mammalian cells and which has the amino acid sequence and at least the decree of purity of the protein produced by the process comprising:growing human WISH cells in culture; reducing the cells with IFN-gamma in a serum-free medium and harvesting the culture supernatant; concentrating said supernatant by ultrafiltration with a membrane of molecular weight cut-off of about 10,000 Da; subjecting said concentrated supernatant to ion exchange chromatography and selecting the fractions exhibiting inhibition of the vesicular stomatitis virus (VSV)-induced cytopathic effect in WISH cells; applying said selected fraction to reversed phase high pressure liquid chromatography (RP-HPLC) at about neutral pH and recovering the fractions exhibiting inhibition of the VSV-induced cytopathic effect in WISH cells; and recovering protein from said selected RP-HPLC fraction, said protein being a substantially purified protein of apparent molecular weight of 40,000 Da by size exclusion chromatography which inhibits viral infection of mammalian cells.