Patent ID: 8795685
Filing Date: 2014-08-05
Classification: A61K,A61P,C07K,C12N,G01N

Abstract:
1. A composition comprising a pharmaceutically acceptable carrier and a mutated Nef protein having substantially no immunosuppressive activity, said mutated Nef protein being obtained by substitution of one amino acid of the immunosuppressive domain of a wild type Nef protein having an immunosuppressive domain that differs from the immunosuppressive domain of a non-mutated wild type Nef protein by a substitution of one amino acid of the immunosuppressive domain of said wild type Nef protein, said wild type Nef protein being constituted by one of the amino acid sequences of the group consisting of SEQ ID NO: 1, SEQ ID NO: 275, SEQ ID NO: 276, SEQ ID NO: 277, SEQ ID NO: 278, SEQ ID NO: 279, SEQ ID NO: 280, SEQ ID NO: 281, SEQ ID NO: 285, SEQ ID NO: 286, SEQ ID NO: 287, SEQ ID NO: 288, SEQ ID NO: 289, or SEQ ID NO: 290, said mutated Nef protein having substantially preserved CD4 and/or MHC-I downregulation functions with respect to the non mutated Nef protein, wherein the one amino acid is selected from the group consisting of: the amino acid at position 93 of one of SEQ ID NO: 1, SEQ ID NO: 275, SEQ ID NO: 277, SEQ ID NO: 278, SEQ ID NO: 279, the amino acid at position 94 of SEQ ID NO: 276, the amino acid at position 96 of SEQ ID NO: 281, the amino acid at position 103 of SEQ ID NO: 280, the amino acid at position 124 of SEQ ID NO: 287, and the amino acid at position 125 of one of SEQ ID NO: 285, SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 289, and SEQ ID NO: 290; the one amino acid is substituted by one of R, H and K; and said mutated NEF protein varies from said wild type NEF protein by said substitution of said one amino acid in said immunosuppressive domain.