Patent ID: 6033646
Filing Date: 2000-03-07
Classification: A61K,A61M,G01R

Abstract:
A method for preparing a fluorine-containing gas-filled microsphere comprising:a. reconstituting the lipids dipalmitoylphosphatidylcholine, dipalmitoylphosphatidylethanolaminepolyethylene glycol, and dipalmitoylphosphatidic acid in a ratio of about 70 to about 90 mole %, about 5 to about 15 mole %, and about 5 to about 15 mole %, respectively, in an aqueous solution to a concentration of about 20 mg/ml to about 50 mg/ml to form a lipid-containing aqueous solution;b. lyophilizing said lipid-containing aqueous solution to form a lyophilized composition such that the dipalmitoylphosphatidylcholine, dipalmitoylphosphatidylethanolamine-polyethylene glycol, and dipalmitoylphosphatidic acid ratio of about 70 to about 90 mole %, about 5 to about 15 mole %, and about 5 to about 15 mole %, respectively, is uniform throughout the composition;c. dispersing said lyophilized composition in an aqueous-based pharmaceutically acceptable carrier to a concentration of about 0.1 mg/ml to about 5 mg/ml to form an aqueous microsphere-forming solution;d. introducing a fluorine-containing gas into said aqueous microsphere-forming solution; ande. shaking said aqueous microsphere-forming solution to form a microsphere filled with fluorine-containing gas.