Patent ID: 7777018
Filing Date: 2010-08-17
Classification: A61K,A61P,C07K

Abstract:
1. A method of making a CDR-grafted antibody capable of inhibiting human tissue factor, wherein the complementarity determining regions (CDRs) are obtained from a murine monoclonal antibody against human tissue factor and the constant (C) and framework (FR) regions are obtained from one or more human antibodies, wherein said CDRs of the heavy chain have the amino acid sequences: and said CDRs of the light chain have the amino acid sequences: wherein the heavy chain comprises residues obtained from the murine monoclonal antibody at positions 23, 24, 28, 29, 30, 48, 49, 71, 88 and 91 and the light chain comprises residues obtained from the murine monoclonal antibody at positions 39 and 105, wherein the residues are numbered according to the Kabat numbering system, comprising cotransfecting a host cell with an expression vector comprising a nucleic acid encoding the CDR-grafted antibody heavy chain and an expression vector comprising a nucleic acid encoding the CDR-grafted antibody light chain; culturing the transfected host cell; and recovering said CDR-grafted antibody.