Patent ID: 8753888
Filing Date: 2014-06-17
Classification: C12N,G01N,Y10T

Abstract:
1. A method of isolating white blood cells from a sample, the method comprising: (a) obtaining a sample comprising whole blood; (b) decreasing viscosity of the sample by diluting the sample or reducing the pH of the sample; (c) adding to the sample a magnetic particle that binds specifically to white blood cells or a subset of white blood cells to provide a white blood cell/magnetic particle complex; (d) isolating the white blood cell/magnetic particle complex by subjecting the white blood cell/magnetic particle complex to a magnetic field at a strength and for a time sufficient to achieve a final cell yield of greater than 75%; and (e) removing the magnetic particles from the complex, thus isolating white blood cells with a cell yield of greater than 75%, wherein the white blood cells have a viability greater than 90% and a purity greater than 80%, wherein at least one of (b)-(e) is automated.