Patent ID: 6066455
Filing Date: 2000-05-23
Classification: C12Q

Abstract:
A method for the detection of nucleic acids in different samples, comprising the steps ofa) amplifying nucleic acids in any samples to be tested,b) adding at least one detergent selected from the group consisting of anionic, non-ionic and zwitterionic detergents to said amplified nucleic acids under alkaline conditions to form reaction mixtures, said detergents being present in a stabilizing amount between 0.01 wt % and 10 wt %, wherein said alkaline conditions are at a pH between 10 and 14,c) incubating said reaction mixtures in plastic vessels at least until any double stranded nucleic acids present in the reaction mixtures are converted into single stranded nucleic acids,d) adding immobilizable or immobilized capture probes to said reaction mixtures,e) hybridizing said single stranded nucleic acids with said capture probes to form immobilized hybrids, andf) determining the amount of immobilized hybrids.