Patent ID: 8097419
Filing Date: 2012-01-17
Classification: C12Q

Abstract:
1. A method for detecting the presence of an Influenza A H1N1 2009 subtype-specific viral nucleic acid segment in a population of polynucleotides, which method comprises: (a) performing a cycling step, wherein the cycling step comprises contacting the population of polynucleotides with a first pair of complimentary amplification primers wherein one primer contains the sequence of SEQ ID NO 51 and the other primer contains the sequence of SEQ ID NO 52 and performing a PCR amplification to produce an Influenza A H1N1 2009 subtype specific amplification product if an Influenza A H1N1 2009 subtype-specific nucleic acid segment is present in the population; and (b) detecting the presence of the amplification product using a first labeled oligonucleotide detection probe that specifically hybridizes to the H1N1 2009 subtype-specific, amplification product, wherein the presence of the amplification product is indicative of the presence of the Influenza A H1N1 2009 subtype-specific viral nucleic acid segment in the population.