Patent ID: 8715926
Filing Date: 2014-05-06
Classification: C12Q,G01N

Abstract:
1. A method of detecting biomarkers associated with head and neck tumors in a patient comprising: (a) providing a first patient sample from the patient, wherein the first patient sample is selected from the group consisting of saliva, whole blood, white blood cells, serum, plasma and biopsy tissue from the throat, oropharynx or mouth of the patient; (b) contacting the first patient sample with: where the first reagent is an oligonucleotide; where the one or more than one HPV biomarker is mRNA; and where the host cell biomarker is MCM8; where the first reagent binds to the one or more than one HPV biomarker if the HPV (human papillomavirus) biomarker is present in the first patient sample, and the second reagent binds to the one or more than one host cell biomarker simultaneously; (c) simultaneously detecting the presence or absence of the HPV biomarker in the first patient sample and determining the expression level of the host cell biomarker in the first patient sample; and (d) comparing the expression level of the host cell biomarker in the first patient sample to an expression level of the host cell biomarker from at least one reference sample, wherein the reference sample is a comparable biological sample obtained from a disease-free subject.