Patent ID: 6986994
Filing Date: 2006-01-17
Classification: C07K,G01N,Y10T

Abstract:
1. A method for assaying islet neogenesis associated protein (INGAP) molecule in a test sample, comprising: a. contacting a solid support comprising bound antibodies with a test sample, wherein the antibodies specifically bind to an amino acid sequence FLSWVEGEESQKKLPSSRITC (SEQ ID NO: 1) of INGAP molecule, whereby INGAP molecule in the test sample binds to the antibodies and thereby to the solid support; b. removing the test sample which has not bound to the antibodies and thereby not bound to the solid support; c. contacting the solid support with a quantity of a labeled INGAP molecule, whereby all or a portion of the quantity of labeled INGAP molecule binds to the antibodies and thereby to the solid support; d. removing labeled INGAP molecule which has not bound to the antibodies and thereby not bound to the solid support; and e. determining the amount of labeled INGAP molecule bound to the solid support wherein the amount of labeled INGAP molecule bound to the antibodies and thereby to the solid support is inversely related to the amount of INGAP molecule in the test sample.