Patent ID: 6905688
Filing Date: 2005-06-14
Classification: A01K,A61K,A61P,C07K,C12N,Y02A

Abstract:
1. An albumin fusion protein comprising a member selected from the group consisting of: (a) a brain derived neurotrophic factor protein and albumin, wherein albumin comprises the amino acid sequence of SEQ ID NO:18; (b) a brain derived neurotrophic factor protein and a fragment of the amino acid sequence of SEQ ID NO:18, wherein said fragment has the ability to prolong the shelf-life of the brain derived neurotrophic factor protein compared to the shelf-life of the brain derived neurotrophic factor protein in an unfused state; (c) a brain derived neurotrophic factor protein and a fragment of the amino acid sequence of SEQ ID NO: 18, wherein said fragment has the ability to prolong the shelf-life of the brain derived growth factor protein compared to the shelf-life of the brain derived neurotrophic factor protein in an unfused state, and further wherein the said fragment comprises amino acid residues 1-387 of SEQ ID NO:18; (d) a fragment of a brain derived neurotrophic factor protein and albumin comprising the amino acid sequence of SEQ ID NO: 18, wherein said fragment has a biological activity of the brain derived neurotrophic factor protein; (e) a brain derived neurotrophic factor protein, or fragment thereof and albumin, or fragment thereof, of (a) to (d), wherein the brain derived neurotrophic factor protein or fragment thereof, is fused to the N-terminus of albumin or the N-terminus of the fragment of albumin; (f) a brain derived neurotrophic factor protein or fragment thereof, and albumin or fragment thereof, of (a) to (d), wherein the brain derived neurotrophic factor protein or fragment thereof, is fused to the C-terminus of albumin, or the C-terminus of the fragment of albumin; (g) a brain derived neurotrophic factor protein or fragment thereof, and albumin or fragment thereof, of (a) to (d), wherein the brain derived neurotrophic factor protein or fragment thereof, is fused to the N-terminus and C-terminus of albumin, or the N-terminus and the C-terminus of the fragment of albumin; (h) a brain derived neurotrophic factor protein or fragment thereof, and albumin or fragment thereof, of (a) to (d), which comprises a first brain derived neurotrophic factor protein or fragment thereof and a second brain derived neurotrophic factor protein or fragment thereof, wherein said first brain derived neurotrophic factor protein or fragment thereof is different from said second brain derived neurotrophic factor protein or fragment thereof; (i) a brain derived neurotrophic factor protein or fragment thereof, and albumin or fragment thereof, of (a) to (h), wherein the brain derived neurotrophic factor protein or fragment thereof, is separated from the albumin or the fragment of albumin by a linker; and (j) a brain derived neurotrophic factor protein or fragment thereof, and albumin or fragment thereof, of (a) to (i), wherein the brain derived neurotrophic factor protein or fragment thereof, wherein the albumin fusion protein has the following formula:  and further wherein R1 is brain derived neurotrophic factor protein or fragment thereof, L is linker, and R2 is albumin comprising the amino acid sequence of SEQ ID NO: 18 or a fragment of albumin.