Patent ID: 6420124
Filing Date: 2002-07-16
Classification: A01K,A61K,A61P,C07K,C12Q

Abstract:
A method to screen for drugs which are useful in treating a person with a mutation in KVLQT1, wherein said mutation is one which, based upon SEQ ID NO:2, results in an Arg at amino acid residue 168, a Ser at amino acid residue 314, a Cys at amino acid residue 315, an Asn at amino acid residue 318, a Pro at amino acid residue 353, a Trp at amino acid residue 366, a Trp at position 167 concurrent with a deletion of amino acid residue 168, a Pro at position 178, an Arg at position 189, a Gln at position 190, a Met at position 254, a Phe at position 273, an Arg at position 306, an Ile at position 312, a Glu at position 341, a Val at position 341 or a Glu at position 345 said method comprising:a) placing a first set of cells expressing KVLQT1 with a mutation, wherein said mutation, based upon SEQ ID NO:2, is an Arg at amino acid residue 168, a Ser at amino acid residue 314, a Cys at amino acid residue 315, an Asn at amino acid residue 318, a Pro at amino acid residue 353, a Trp at amino acid residue 366, a Trp at position 167 concurrent with a deletion of amino acid residue 168, a Pro at position 178, an Arg at position 189, a Gln at position 190, a Met at position 254, a Phe at position 273, an Arg at position 306, an Ile at position 312, a Glu at position 341, a Val at position 341 or a Glu at position 345 into a bathing solution to measure a first induced K+ current; b) measuring said first induced K+ current; c) placing a second set of cells expressing wild-type KVLQT1 into a bathing solution to measure a second induced K+ current; d) measuring said second induced K+ current; e) adding a drug to the bathing solution of step (a); f) measuring a third induced K+ current of cells in step (e); and g) determining whether the third induced K+ current is more similar to the second induced K+ current than is the first induced K+ current, wherein drugs resulting in a third induced K+ current which is closer to the second induced K+ current than is the first induced K+ current are useful in treating said persons.