Patent ID: 8969059
Filing Date: 2015-03-03
Classification: B01D,B01L,B03C,C12N,C12Q,G01N,G01S,G06F

Abstract:
1. A method of isolating a nucleic acid from a fluid comprising cells, comprising a. applying the fluid to a device, the device comprising an array of electrodes capable of generating an AC electrokinetic field, wherein the first AC electrokinetic region is a first dielectrophoretic field region; b. concentrating a plurality of cells in a first AC electrokinetic field region; c. isolating nucleic acid in a second AC electrokinetic field region, wherein the second AC electrokinetic region is a second dielectrophoretic field region; d. flushing the plurality of cells away in the first AC electrokinetic field; e. performing PCR amplification on the nucleic acid, or a cDNA version of the nucleic acid, to produce a PCR product; and f. isolating the PCR product in a third AC electrokinetic region.