Patent ID: 7413905
Filing Date: 2008-08-19
Classification: G01N,Y10T

Abstract:
1. A reagent for classifying leukocytes into four groups consisting of three groups corresponding to lymphocytes, monocytes and eosinophils and one group corresponding to neutrophils and basophils, the reagent comprises: (a) at least one surfactant for lysing erythrocytes and partly damaging the cell membrane of leukocytes, the at least one surfactant is selected from the group consisting of: one or more cationic surfactants; one or more anionic surfactants; one or more ampholytic surfactants; a combination of one or more cationic surfactants and one or more ampholytic surfactants; and a combination of one or more anionic surfactants and one or more ampholytic surfactants; (b) at least one organic compound bearing an anionic group for binding with a cationic component present in the leukocytes to give morphological differences between the leukocytes; and (c) a buffer for adjusting pH into a range of 2˜8; wherein the reagent is free from nonionic surfactants.