Patent ID: 7208293
Filing Date: 2007-04-24
Classification: A61K,C07K,C12N,C40B

Abstract:
1. A method of obtaining a nucleic acid encoding a proteinaceous binding domain that binds a target material comprising: a) providing a variegated population of eukaryotic cells, each cell including a nucleic acid that encodes a potential binding protein, wherein the nucleic acid comprises DNA encoding (i) a potential binding domain having one or more variable residue positions and one or more non-variable residue positions, and (ii) an outer surface transport signal for obtaining the display of the potential binding domain on the outer surface of the cell, wherein said variegated population of cells collectively display a plurality of different potential binding domains, the differentiation among said potential binding domains, occurring as a result of amino acid variation at said one or more variable residue positions, (b) allowing the expression of said potential proteins and the display of said potential binding domains on the outer surface of said cells; (c) contacting said cells with the target material such that said potential binding domains and the target material may interact; (d) separating cells displaying a potential binding domain that binds the target material from cells that do not so bind, wherein a potential binding domain that binds the target material is a successful binding domain (SBD) and (e) recovering at least one cell displaying on its outer surface a binding protein comprising an SBD, said cell enclosing SBD-encoding nucleic acid, and amplifying said SBD-encoding nucleic acid in vivo or in vitro.