Patent ID: 7264931
Filing Date: 2007-09-04
Classification: C12Q

Abstract:
1. A method for sequencing a nucleic acid sequence comprising: (a) linearly amplifying the nucleic acid sequence using a first primer which is complementary to a known portion of the nucleic acid sequence to produce a first primer extended product; (b) incubating the first primer extended product with dNTPs, a DNA polymerase, and separate pools of semi-random oligonucleotide primers consisting of 5′ (N) (c) incubating each of the four pools of primer extended products with a second detectably labeled primer which is complementary to a known portion of the nucleic acid sequence to produce detectably labeled extension products via linear amplification; and (d) separating the detectably labeled extension products by size; and (e) reading the sequence of the detectably labeled extension products via an automated sequencing machine.