Patent ID: 6762046
Filing Date: 2004-07-13
Classification: C12N,C12P,C12Y,G01N

Abstract:
An isolated DNA encoding a polypeptide having beta-fructofuranosidase activity, said polypeptide obtainable by the expression of a Bacillus gene, which polypeptide contains the amino acid sequences of SEQ ID NO:1 and SEQ ID NO:2 as an amino acid sequence and which polypeptide has the following physicochemical properties:a. Molecular weight About 44,000-55,000 Daltons determined by SDS-PAGE; b. Optimum pH About 5.5-6.0 when incubated at 40Â° C. for ten minutes; c. Optimum temperature About 45Â° C. in the absence of calcium ion when incubated at pH 6.0 for ten minutes, and about 50Â° C. in the presence of calcium ion when incubated at pH 6.0 for ten minutes; d. pH Stability Stable at a pH of about 5.0 to 8.0 when incubated at 40Â° C. for 24 hours; and e. Thermal stability Stable up to a temperature of about 45Â° C. when incubated at pH 6.0 for one hour.