Patent ID: 9103779
Filing Date: 2015-08-11
Classification: B01D,G01N

Abstract:
1. An electrophoresis gel comprising an electrolyte solution comprising: Tris(hydroxymethyl)aminomethane (TRIS); at least one zwitterion chosen from 2-amino-2methyl-1,3-propanediol (AMPD), N,N-bis[2-hydroxyethyl]-2-aminoethanesulphonic acid (BES), N-Glycylglycine (Gly-Gly), 4-(2-hydroxyethyl)piperazine-1-propanesulfonic acid (EPPS or HEPPS), 3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid (CAPSO), N-(1,1-Dimethyl-2-hydroxyethyl)-3-amino-2-hydroxypropanesulfonic acid (AMPSO), 3-(cyclohexylamino)-1-propanesulfonic acid (CAPS), 2-(cyclohexylamino)ethanesulfonic acid (CHES), 3-[N-Bis(hydroxyethyl)amino]-2-hydroxypropanesulfonic acid (DIPSO), N-(2-Hydroxyethyl)piperazine-N′-(4-butanesulfonic acid) (HEPBS), 4-(N-Morpholino)butanesulfonic acid (MOBS), 3-N-Morpholino propanesulfonic acid (MOPS), piperazine-N,N′-bis[2-hydroxypropanesulphonic]acid (POPSO), N-Tris(hydroxymethyl)methyl-4-aminobutanesulfonic acid (TABS), N-Tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid (TAPS), (2-[2-hydroxy-1,1-bis(hydroxymethyl)ethylamino]ethanesulphonic acid) (TES), (2-[2-acetamino]-2-aminoethanesulphonic acid) (ACES), (1,4-piperazinediethanesulphonic acid) (PIPES), and piperazine-N,N′-bis(3-propanesulfonic Acid) (PIPPS); water; sodium dodecyl sulfate (SDS); and methanol; wherein said electrolyte solution is for accelerating, or improving resolution, or improving transfer efficacy, or accelerating and improving resolution, or accelerating and improving transfer efficacy of gel electrophoresis; wherein said gel is chosen from an acrylamide gel at a concentration from less than 1% (wt/vol) to about 25% (wt/vol) or an agarose gel at a concentration from about 0.5% (wt/vol) to about 3% (wt/vol); and wherein the pH of said gel is from about 8.0 to about 8.8.