Patent ID: 6686515
Filing Date: 2004-02-03
Classification: C07K,C12N

Abstract:
A method of evolving a recombinase to complement an Agrobacterium virE2 gene, the method comprising:(a) providing a population of nucleic acids, wherein the population encodes a plurality of recombinase genes; (b) recombining or mutating the population of nucleic acids to generate a library of recombinant nucleic acid segments; (c) optionally repeating the recombining or mutating process of steps (a) and (b) one or more times; and (d) screening the library of recombinant nucleic acid segments, which recombinant nucleic acid segments comprise evolved recombinase genes, to identify at least one recombinant nucleic acid segment encoding an evolved recombinase, which evolved recombinase can substitute functionally for an Agrobacterium virE2 gene in plant transformation.