Patent ID: 7670829
Filing Date: 2010-03-02
Classification: A61P,C12P,C12Y

Abstract:
1. A Saccharomyces cerevisiae yeast strain genetically modified to autonomously produce, from a simple carbon source, a steroid or steroid derivative derived from cholesterol metabolism, wherein said steroid or steroid derivative is selected from the group consisting of pregnenolone, 17α-hydroxypregnenolone, cortisol, cortexolone, progesterone, 17α-hydroxyprogesterone, and derivatives thereof; wherein the genetic modification comprises: a) the inactivation of ATF2, GCY1 and YPR1, and b) at least one heterologous gene or cDNA in an expression cassette, wherein the gene or cDNA is selected from the group consisting of the gene or cDNA encoding