Patent ID: 6830897
Filing Date: 2004-12-14
Classification: C12Q,G01N,Y10S

Abstract:
A high throughput discovery screen for determining the potential toxicity of candidate drugs comprising the steps of:(a) incubating the candidate drugs, in a first multi-well plate, with a microsomal enzyme metabolizing system wherein said drugs may form reactive electrophilic metabolites; (b) permitting said electrophilic metabolites to react with glutathione in order to form glutathione conjugates; (c) processing the samples, containing glutathione-drug cpnjugates, via an automated solid phase extraction system, in order to prepare a further multi-well plate from which the conjugates can be extracted for automated HPLC; (d) conducting HPLC column runs on said extracts in order to generate HPLC eluates containing said conjugates; (e) introducing said eluates, at source, into a triple quadrapole mass spectrometer, and (f) detecting said conjugates with tandem (MSâ€”MS) mass spectrometry.