Patent ID: 6063572
Filing Date: 2000-05-16
Classification: C12Q

Abstract:
A method for detecting a target nucleic acid, comprising:(a) adding to a sample an oligonucleotide primer, wherein the nucleotide sequence of the oligonucleotide primer is complementary to the target nucleic acid sequence and comprises a RNA polymerase promoter sequence, wherein a duplex is formed between the target nucleic acid and the primer when the target sequence is present in the sample;(b) elongating the primer in a duplex with the target nucleic acid sequence in (a) to produce a double stranded polynucleotide;(c) reacting the double stranded polynucleotide produced in (b) with RNA polymerase to produce RNA;(d) adding a oligonucleotide probe complementary to the RNA in (c), wherein the probe is labeled with a fluorescent intercalative dye;(e) measuring the fluorescence of the intercalative dye.