Patent ID: 8628925
Filing Date: 2014-01-14
Classification: C12P,C12Q

Abstract:
1. A method of linearly amplifying a multiplicity of different short nucleic acid sequences, wherein the multiplicity of short nucleic acid sequences are 15-35 nucleotides in length, said method comprising; forming a reaction mixture comprising a multiplicity of different short nucleic acid sequences, a collection of target-specific primers, and an enzyme that catalyzes target specific primer extension, wherein the short nucleic acid sequences are 15-35 nucleotides in length; annealing the collection of target-specific primers to the multiplicity of different short nucleic acid sequences; extending the multiplicity of target-specific primers with the enzyme that catalyzes target-specific primer extension; and cycling the reaction mixture for at least 20 cycles between an annealing temperature segment of 10° C.-30° C., an extension segment, and a denaturation temperature segment of 35° C.-60° C.; thereby linearly amplifying the multiplicity of different short nucleic acid sequences.