Patent ID: 8492146
Filing Date: 2013-07-23
Classification: A61K,A61P,C12N

Abstract:
1. A method for producing a human T cell population which has both cytotoxic and immunosuppressive activities, said method comprising the following steps (1) to (5): (1) fractionating mononuclear cells collected from a human umbilical cord blood into CD14-positive (CD14 (2) co-culturing the CD2-positive CD14-negative (CD2 (3) co-culturing the CD14-positive (CD14 (4) adding the blast cells obtained in step (2) to the resulting co-culture of step (3) to proliferate the blast cells obtained in step (2); and (5) co-culturing the blast cells obtained in step (4) with fresh stromal cells in medium supplemented with interleukin-2 (IL-2) to further proliferate, thereby producing a human T cell population which has both cytotoxic and immunosuppressive activities.