Patent ID: 6307028
Filing Date: 2001-10-23
Classification: A61L,C07K

Abstract:
A method of preparing a purified, virally inactivated antibody preparation from a starting solution comprising antibodies and other substances at an initial pH, the method comprising the sequential steps a) through e) ofa) adjusting the pH of the starting solution to be within a range of from about 3.8 to about 4.5 to form an intermediate solution comprising dissolved antibodies,b) adding a source of caprylate ions to the intermediate solution of step a) and adjusting the pH of the intermediate solution to be within a range of from about 5.0 to about 5.2 to form a precipitate and a supernatant solution comprising dissolved antibodies,c) incubating the supernatant solution under conditions of time, temperature and caprylate ion concentration to inactivate substantially all enveloped viruses,d) contacting the supernatant solution with at least one ion exchange resin under conditions that allow binding of at least some of the other substances including IgA or IgM to the resin while not allowing binding of the antibodies including IgG to the resin, ande) collecting the IgG antibodies, the method further comprising the non-sequential step f) of,f) eluting IgA or IgM from the ion exchange resin column;wherein the precipitate in step b) comprises non-enveloped viruses, resulting in a significant reduction of non-enveloped viruses.