Patent ID: 7262006
Filing Date: 2007-08-28
Classification: C12Q

Abstract:
1. A method for isolating a free circulating, extracellular nucleic acid from a mammalian species sample not previously treated with a cell lysing reagent, wherein said method comprises the steps of: A) admixed in buffer at a pH of less than 7, contacting a sample suspected of containing a nucleic acid with a water-soluble, weakly basic polymer derived by addition polymerization of: B) separating said water-insoluble precipitate from said sample, and C) contacting said precipitate with a base to raise the buffer pH to greater than 7, and thereby releasing said free circulating, extracellular nucleic acids from said weakly basic polymer, said weakly basic polymer derived by addition polymerization of one or more ethylenically unsaturated polymerizable monomers having an amine group which can be protonated at acidic pH.