Patent ID: 9068186
Filing Date: 2015-06-30
Classification: C12N,C12P,C40B

Abstract:
1. An isolated, viable, non-conditional, Gram-negative mutant bacterium, wherein said bacterium is Escherichia coli and comprises an outer membrane that substantially lacks a ligand that acts as an agonist of a human TLR4/MD-2 receptor, said ligand comprising lipid A, wherein the bacterium comprises (a) a mutation in at least one of the genes selected from the group consisting of kdsA, kdsC, kdsB, and waaA, or a mutation in each of the kdsD and gutQ genes, wherein said mutation results in a disruption in the biosynthesis of (Kdo) 2 -lipid IV A , (b) a mutation in each of the genes pagP and eptA wherein said mutation substantially inactivates the enzymatic activity of each of the proteins encoded by the pagP and eptA genes, and (c) a suppressor mutation in the msbA gene resulting in an increased capacity of the encoded MsbA protein to transport lipid IV A .