Patent ID: 7361751
Filing Date: 2008-04-22
Classification: A01N,C07K,C12N,C12R,Y02A

Abstract:
1. An isolated DNA encoding a variant of the protein of SEQ ID) NO:5, having substantially the same insecticidal activity as the protein of SEQ ID NO:5, or an insecticidally-effective fragment thereof, which variant comprises an amino acid sequence encoded by a DNA hybridizing under stringent hybridization conditions to the DNA of SEQ ID NO:4 from nucleotide position 797 to nucleotide position 2641, wherein said stringent hybridization conditions are established as follows using the following consecutive steps: a. immobilizing DNA fragments on a filter; b. prehybridizing said filter for 1 to 2 hours at 42° C. in 50% formamide, 5×SSPE, 2× Denhardt's reagent and 0.1% SDS, or for 1 to 2 hours at 68° C. in 6×SSC, 2 X Denhardt's reagent and 0.1% SDS; c. adding a hybridization probe, which has been radiolabeled; d. incubating for 16 to 24 hours; e. washing said filter for 20 minutes at room temperature in 1×SSC, 0.1% SDS; f washing said filter three times for 20 minutes each at 68° C. in 0.2×SSC, 0.1% SDS; and g. autoradiographing said filter by exposing said filter for 24 to 48 hours to X-ray film at −70° C. with an intensifying screen.