Patent ID: 6916604
Filing Date: 2005-07-12
Classification: C07K,G01N,Y10S

Abstract:
1. A method of assaying type I collagen fragments in a body fluid sample, comprising: (a) contacting the body fluid with a synthetic peptide consisting essentially of either the type I collagen α1(I) N-telopeptide sequence Asp-Glu-Lys-Ser-Thr-Gly-Gly (SEQ ID NO:5) or the α2(I) N-telopeptide sequence Gln-Tyr-Asp-Gly-Lys-Gly-Val-Gly (SEQ ID NO:6), and an immunological binding partner immunoreactive with said amino acid sequence, wherein the collagen fragments compete with said synthetic peptide for binding with the immunological binding partner; and (b) quantifying the amount of collagen fragments in the sample by measuring the amount of binding of said immunological binding partner with said collagen fragments.