Patent ID: 6297006
Filing Date: 2001-10-02
Classification: C12Q,Y10T

Abstract:
A method for sequencing a repetitive sequence in a target nucleic acid comprising the steps of:providing a plurality of spacer oligonucleotides of varying lengths wherein each spacer oligonucleotide in said plurality of spacer oligonucleotides includes at least one repeat of said repetitive sequence;providing a first oligonucleotide that is adjacent to a first end of the repetitive sequence;providing a plurality of second oligonucleotides at least one of which is adjacent to a second end of the repetitive sequence, wherein each oligonucleotide in the plurality of second oligonucleotides is labeled;hybridizing the first oligonucleotide, one of the plurality of second oligonucleotides, and one of the plurality of spacer oligonucleotides to the target nucleic acid under conditions that discriminate a perfect match hybrid from a base pair mismatch hybrid;ligating the hybridized oligonucleotides;separating ligated oligonucleotides from unligated oligonucleotides; anddetermining a sequence of the repetitive sequence by identifying the spacer oligonucleotide and the second oligonucleotide in the ligated oligonucleotides.