Patent ID: 7604955
Filing Date: 2009-10-20
Classification: A01K,A61K,A61P,C07K,G01N

Abstract:
1. A method of identifying natural immunoglobulin E peptides presented by major histocompatibility complex class I molecules on IgE-producing cells that induce a cytotoxic T-lymphocyte response against natural IgE, comprising: a) immunizing a first group of mice with an IgE peptide and a co-stimulatory factor, b) performing one or more essays to detect an immune response specific for the IgE peptide administered in step a) wherein the assays are chosen from the group consisting of: wherein detection of a cytotoxic T-lymphocyte response, a decreased level of circulating IgE as compared to a control and/or a decreased number of IgE-producing cells as compared to a control indicates that the IgE peptide of step a) comprises a natural IgE peptide, c) generating peptides 8 to 10 amino acids in length which are truncations of the IgE peptide of step a), d) immunization a second group of mice with one of the peptides of step c) and e) performing one or more assays to detect an immune response specific for the IgE peptide administered in step d) wherein the assays are chosen from the group consisting of: wherein detection of a cytotoxic T-lymphocyte response, a decreased level of circulating IgE as compared to a control and/or a decreased number of IgE-producing cells are compared to a control indicates that the IgE peptide of step d) is a natural IgE peptide.