Patent ID: 6140122
Filing Date: 2000-10-31
Classification: A61K,C12N,Y10S

Abstract:
A kit for obtaining compositions of macrophages, or MD-APCs, comprising:necessary elements for washings, culture and elutriation, including:at least one bag for washing,at least one culture bag,at least one connector for eliminating supernatant from the washings,at least one connecting tube between the preceding bags,at least one injection site in the washing and culture bags,at least one system allowing for opening and closing of the at least one connecting tube between the at least one washing bag and the at least one culture bag,two bags containing a physiologic solution for the washings and the elutriation,a three branch transfuser with air intake,a silicon tube and a joint on a rotor serving as a connection between the transfuser and the rotor of the elutriator,at least one collection bag,a tube and a joint on the rotor serving as a connection between the at least one collection bag and the rotor of the elutriator,necessary reactive agents for the culture, differentiation, activation and control, including:a bag of specific, ready to use culture medium, to which has been added L-Glutamine (2 mM, Gibco) or L-Alanyl-L-Glutamine (2 Mm, Gibco), pyruvic acid (2 mM, Gibco), Indomethacine (5.times.10.sup.-6 M, Sigma), mercaptoethanol (3.times.10.sup.-5 M, Gibco), non-essential amino acids (2%, Gibco); 2 to 5% of one of AB.sup.+ serum and autologous serum,at least one supplement to be added to the culture medium, selected from a group consisting of:GMSCF,vitamin D3,interleukin-13 (IL-13)IL-4TNF-.alpha.histamineat least one internal control comprising at least one of monocytes, and macrophages or MD-APCs,at least one labelled antibody.