Patent ID: 6159712
Filing Date: 2000-12-12
Classification: A61K,C07K,C12N,C12P,C12Q

Abstract:
A method for producing .alpha.- or .beta.-interferon in a human cell culture, in the absence of viral induction, comprising:(a) culturing a human cell line capable of producing interferon and transfected with a vector containing DNA encoding duble-stranded-RNA-dependent kinase PKR under the control of a promoter, under culture conditions in which PKR is overproduced in the transfected cells, as evidenced by levels of PKR in said transfected cell line which are higher than those obtained in said human cell line which is not transfected with said vector, when grown under the same culture conditions,(b) treating said cultured, PKR-overproducing human cell line with double-stranded RNA (dsRNA), in the absence of viral induction, and(c) collecting .alpha.- or .beta.-interferon produced by the cultured, treated cell line.