Patent ID: 7846694
Filing Date: 2010-12-07
Classification: C07K,C12N,C12Q

Abstract:
1. A method of producing an amplified DNA fragment comprising: i) amplifying a linear double-stranded or single-stranded DNA by polymerase chain reaction (PCR), using a reaction solution comprising: ii) amplifying the first amplified DNA fragment by polymerase chain reaction (PCR), using a reaction solution comprising: b) a sense primer which anneals with the 5′ terminal region of the second DNA fragment; and and wherein the DNA fragment ac) has a 3′-terminal sequence that is the complement of the 5′ terminal sequence of the DNA fragment ab) and the sense primer b) is the same as the anti-sense primer c); thereby obtaining an amplified DNA molecule comprising the overlapped DNA fragments aa), ab) and ac).