Patent ID: 6617442
Filing Date: 2003-09-09
Classification: C07H,C07K,C12N

Abstract:
A mixed sequence oligonucleotide comprising at least 12 nucleotides and having a 3â€² end and a 5â€² end and divided into a first portion and a further portion,said first portion supports cleavage of a complementary target RNA by human RNase H1 polypeptide, said further portion that does not support said cleavage by said RNase H1; wherein: said first portion comprises at least 6 nucleotides and is positioned in said oligonucleotide such that at least one of said 6 nucleotides is 8 to 12 nucleotides from the 3â€² end of said oligonucleotide; said first portion comprises at least one 5â€²-CA-3â€² nucleotide sequence that is positioned 8 to 12 nucleotides from the 3â€² end of said oligonucleotide, and each nucleotide of said first portion is, independently, a 2â€²-CN arabinonucleotide, a 2â€²-Cl arabinonucleotide, a 2â€²-Br arabinonucleotide, a 2â€²-N3 arabinonucleotide, a 2â€²-OH arabinonucleotide, a 2â€²-Oâ€”CH3 arabinonucleotide or a 2â€²-dehydro-2â€²-CH3 arabinonucleotide.