Patent ID: 8927258
Filing Date: 2015-01-06
Classification: G01N

Abstract:
1. An integrated device for diagnostic analyses used to verify the presence of bacteria in at least a biological sample mixed with a eugonic culture medium in a liquid form, to classify at least the type of bacteria, and to test a series of antibiotics selected from a group of characteristic antibiotics at least for said type of bacteria identified, thereby identifying those that are effective for an antibiotic therapy, the integrated device comprising, inside an integrated structure: a first container that is provided with containing elements in which the biological samples to be analyzed are distributed; a second container comprising at least one of a plurality of recipients and a plurality of micro-plates thermostated with wells, a first examining device that is configured to verify the presence of bacteria in said biological samples contained in said first container and dispensed in the recipients and/or the micro-plates with wells to detect corresponding positive biological samples and to classify and/or identify at least a type of bacteria present in said positive biological samples in order to select a group of antibiotics appropriate for treating the classified or identified type of bacteria; a second examining device that is configured to verify, on at least one of said first recipient and said first well and on at least one of said second recipient and said second well, a sensitivity and/or a resistance of each positive biological sample to a series of antibiotics of the group of antibiotics selected by said first examining device; a third container comprising plates containing a solid culture ground in which a second fraction of the biological sample corresponding to a sample found positive by said first examining device is sown in order to isolate and/or identify said bacteria; a movement and selection unit governed by a control unit to automatically perform at least taking of the positive biological samples and distribution of the positive biological samples in said second container and in said third container; wherein said first examining device comprises a reading system that is configured to detect an attainment of a given turbidity value of a bacterial suspension present in at least one of said recipients and micro-plates with wells obtained during an exponential bacterial growth step, the given turbidity value being 0.5 on the McFarland scale; and a fourth container in which at least a part of the primary biological sample contained in said first container is deposited using the movement and selection unit, wherein the fourth container comprises one or more refrigerated plates having wells, in which part of the primary biological sample is preserved; wherein the integrated device is configured to use a calibrated loop-shaped tube to take from the fourth container and sow on Petri dishes, native or primary biological samples found to be positive to a culture test in a liquid culture ground in the second container, and the integrated device is configured to assess the correctness of the examination carried out by the first examination device.