Patent ID: 6929909
Filing Date: 2005-08-16
Classification: A01K,C12N

Abstract:
1. A targeting vector capable of modifying or disrupting a target gene through homologous recombination, said vector comprising: a) a first sequence capable of homologously recombining with a first region of the target gene; b) a second sequence capable of homologously recombining with a second region of the target gene; c) a selectable marker cassette comprising a DNA sequence encoding a positive selection marker, said cassette located between the first sequence and second sequence; and d) a regulator sequence encoding an element capable of repressing expression of the DNA sequence encoding the selection marker; said regulator sequence located adjacent to the first sequence or second sequence, on a side opposite of the selectable marker cassette; where homologous recombination of the first sequence and second sequence with the target gene results in expression of the selection marker; and where random insertion of the vector into the target gene results in repression of the DNA sequence encoding the selection marker.