Patent ID: 7598081
Filing Date: 2009-10-06
Classification: C07K,C12N,Y10S

Abstract:
1. A method for isolating target cells from a sample, said method comprising i) contacting said sample with a conductive immunopolymer matrix, wherein said conductive immunopolymer matrix comprises: a) a first immunopolymer layer comprising a polyheteroaromatic polymer and an Fc receptor immobilized within said polymer, wherein a first antibody is bound to said Fc receptor in said first polymer and said first antibody binds to a molecule expressed on a first target cell; and b) a second immunopolymer layer comprising a polyheteroaromatic polymer and an Fc receptor immobilized within said polymer, wherein a second antibody is bound to said Fc receptor in said second polymer and said second antibody binds to a molecule expressed on a second target cell; and wherein said first and second immunopolymer layers further comprise a cell monitoring system for monitoring cell binding by said first or second antibody or cell release from said first or second antibody, wherein said cell monitoring system comprises horseradish peroxidase and glucose oxidase entrapped within said first and second immunopolymers; ii) removing said sample from contact with said conductive immunopolymer matrix, wherein said first target cell is bound to said first antibody in said first immunopolymer layer and said second target cell is bound to said second antibody in said second immunopolymer layer, thereby isolating said target cells from said sample; and iii) washing said conductive immunopolymer matrix to remove unbound cells.