Patent ID: 7083987
Filing Date: 2006-08-01
Classification: C40B,G01N,Y10S

Abstract:
1. A method for analyzing a complex protein mixture, said method comprising: labeling one or more active target proteins present in said complex protein mixture by combining at least one probe specific for one or more active target proteins with said complex protein mixture under conditions whereby said probe(s) covalently react with said active target proteins; isolating one or more of said labeled active target proteins by binding to a receptor bound to a solid phase, wherein said receptor binds the probe labeling said active target proteins, removing unbound proteins, and releasing bound labeled active target proteins from said receptor; and detecting a signal from one or more labeled active target proteins present in said complex protein mixture following said isolating, wherein said signal is detected by separating one or more of said labeled active target proteins and generating a fluorescent signal from one or more of said labeled active target proteins during or following said separation; wherein said probe has the structure: wherein: X is —CH R is —H or a chain of from 1–20 carbon atoms and from 0 to 5 heteroatoms, which chain is straight or branched alkyl, alkenyl, or alkynyl optionally comprising one or more aromatic, alicyclic, heteroaromatic, or heterocyclic groups; L is a linker moiety comprising from about 2 to 20 carbon atoms and having from 0 to 10 heteroatoms, wherein L is aliphatic, alicyclic, aromatic or heterocyclic; and Fl is a fluorescent moiety and F is fluorine.