Patent ID: 6673560
Filing Date: 2004-01-06
Classification: C12Q,G01N,Y10S,Y10T

Abstract:
A method for determining the amount of hydride produced in a reaction mixture wherein hydride is generated comprising (a) adding a known amount of a chemiluminescent compound to said reaction mixture, (b) adding reagents to said reaction mixture in step (a) wherein said reagents cause the release of chemiluminescence from said chemiluminescent compound, (c) measuring the amount of chemiluminescence given off from said reaction mixture after the addition of said reagents which release chemiluminescence, (d) comparing (1) the amount of chemiluminescence produced with (2) the expected chemiluminescence in the absence of said hydride to determine the difference in amount of chemiluminescence between (d)(1) and (d)(2), and (e) calculating the amount of said hydride present by comparison of said difference in chemiluminescence with a standard curve,wherein said measuring the amount of chemiluminescence in step (c) does not involve the use of peroxidase and wherein no hydrogen peroxide is produced in said method.