Patent ID: 6399333
Filing Date: 2002-06-04
Classification: C07K,Y10S

Abstract:
A process for the production of a preparation comprising a protein with erythropoietin activity, comprising the steps ofexpressing DNA coding for the protein in a mammalian host cell by a fermentation step of the mammalian host cell without any contact between serum and the mammalian host cell such that all DNA expression occurs in a serum free medium which is free of nonrecombinant mammalian proteins, chromatographically purifying the protein from the cell supernatant by dye affinity chromatography, chromatography on hydroxyapatite, reversed phase chromatography and anion exchange chromatography, wherein one of the chromatography steps carried out after the dye affinity chromatography is a hydrophobic chromatography, and then isolating said protein with erythropoietin activity, wherein said protein is in the form of a preparation which contains less than 100 ppm of proteins derived from the host cell, and less than 10 pg of host cell DNA per 83 &mgr;g erythropoietin and wherein the preparation is completely free of nonrecombinant mammalian proteins which are not derived from the host cell.