Patent ID: 7625744
Filing Date: 2009-12-01
Classification: C12Q,G01N

Abstract:
1. A biologically based test system for detection of an inorganic pollutant selected from the group consisting of arsenite, arsenate and antimonite in water samples, comprising a genetically modified bacterium producing a marker protein upon induction of a regulatory protein, wherein activity of said regulatory protein is induced by said pollutant, wherein said regulatory protein is ArsR, wherein said genetically modified bacterium comprises a first DNA binding site for said regulatory protein positioned in front of a gene encoding said regulatory protein and a second DNA binding site for said regulatory protein positioned between the gene encoding said regulatory protein and a gene encoding said marker protein, and wherein said marker protein is selected from the group consisting of alkaline phosphatase (PhoA), chloramphenicol acetyl transferase (CAT-gene), catechol dioxygenase (XylE), bacterial luciferase (LuxAB), eukarvotic luciferase (Luc) beta-galactosidase (LacZ), and green fluorescent protein (GEP).