Patent ID: 9188593
Filing Date: 2015-11-17
Classification: B01L,G01N

Abstract:
1. A method of assaying for a binding interaction between a protein secreted by a cell and a biomolecule, the method comprising: (a) retaining the cell secreting the protein within a chamber having a solid wall defining the chamber, an inlet and an outlet, wherein the volume of the chamber is from 100 pL to 100 nL; (b) exposing the protein secreted by the cell to a capture substrate, wherein the capture substrate is in fluid communication with the protein secreted by the cell and wherein the capture substrate is operable to bind the protein secreted by the cell to produce a bound protein; (c) flowing a first fluid volume comprising the biomolecule through the inlet into the chamber over the bound protein and out the outlet, wherein the first fluid volume is in fluid communication with the capture substrate and the bound protein; and (d) determining a binding kinetic measurement between the protein secreted by the cell and the biomolecule wherein the binding kinetic measurement is a K