Patent ID: 6472175
Filing Date: 2002-10-29
Classification: C12N,C12P

Abstract:
A method of expressing a recombinant nucleic acid in an insect cell line, using a baculovirus expression system, such that said recombinant nucleic acid being expressed in said baculovirus expression system codes for a protein with N-linked glycosylation, comprising the steps of:a) culturing cells for use in said baculovirus expression system; b) infecting said cells with a recombinant baculovirus that expresses said recombinant nucleic acid encoding a recombinant protein that would normally have N-linked glycosylation if expressed in the source organism of said recombinant nucleic acid; and c) culturing said infected cells in media supplemented with serum and dexamethasone or N-acetylmannosamine, such that said recombinant protein is expressed.