Patent ID: 6962778
Filing Date: 2005-11-08
Classification: C07H,C12Q

Abstract:
1. A method for suppressing the binding of a detectable probe to a non-target sequence in an assay of a sample for a target sequence, the method comprising the steps of: a. contacting the sample with a set containing two or more probes under conditions suitable for the probes to hybridize to nucleic acid, wherein, at least one of said probes is a detectable probe labeled with a detectable moiety and having a sequence complementary or substantially complementary to the target sequence, and at least one of the other probes is an unlabeled or independently detectable probe having a sequence complementary or substantially complementary to a non-target sequence which may be present in the sample; provided that at least one of the detectable probe and the unlabeled or independently detectable probe is a PNA probe; and b. detecting the presence or amount of target sequence present in the sample by directly or indirectly detecting or quantitating the detectable moiety of said detectable probe which hybridized to the target sequence.