Patent ID: 6727062
Filing Date: 2004-04-27
Classification: C07K,C12N,C40B,G01N

Abstract:
A method to acquire a monoclonal antibody or scFv/Fab antibody fragment against a target structure comprising the steps of:(A) exposing a first mounted tissue to an initial antibody library or scFv/Fab antibody fragment library, members of the antibody library or members of scFv/Fab antibody fragment library, is linked to a filamentous phage, a virus, a polysome, or a coded bead comprising an antibody- or scFv/Fab antibody fragment-encoding sequence; (B) eluting directly from the first mounted tissue unbound elements, wherein the unbound elements comprise a first enriched library; and/or recovering a second enriched library comprising the filamentous phage, virus, polysome, or coded bead cleaved from bound elements in the first mounted tissue by cleaving the bound elements from the filamentous phage, virus, polysome, or coded bead; (C) amplifying either the first or second enriched libraries; (D) exposing the first or second enriched libraries to a second mounted tissue, wherein the second mounted tissue represents a different physiological or pathological state than the first mounted tissue; (E) eluting directly from the second mounted tissue unbound elements of the second enriched library from the second mounted tissue, wherein the unbound elements comprise a third enriched library; and/or recovering a fourth enriched library comprising a filamentous phage, virus, polysome, or coded bead of the first enriched library bound to the second mounted tissue section by cleaving the bound elements from the filamentous phage, virus, polysome, or coded bead of the first enriched library; (F) amplifying either the third or fourth enriched libraries; and (G) isolating the monoclonal antibody or scFv/Fab antibody fragment from either the third or fourth enriched libraries.