Patent ID: 8455632
Filing Date: 2013-06-04
Classification: C12Q

Abstract:
1. A kit for the detection of two or more types of human papillomavirus (HPV), comprising two or more combinations selected from the group consisting of (1) a combination of primer set 1 consisting of a primer consisting of the base sequence of SEQ ID NO: 1 and a primer consisting of the base sequence of SEQ ID NO: 2, and a probe consisting of a base sequence with not less than 95% identity with the base sequence of SEQ ID NO: 49, or a probe consisting of a base sequence with not less than 95% identity with the base sequence complementary to the base sequence of SEQ ID NO: 49 (probe 1), (2) a combination of primer set 2 consisting of a primer consisting of the base sequence of SEQ ID NO: 3 and a primer consisting of the base sequence of SEQ ID NO: 4, and a probe consisting of a base sequence with not less than 95% identity with the base sequence of SEQ ID NO: 50, or a probe consisting of a base sequence with not less than 95% identity with the base sequence complementary to the base sequence of SEQ ID NO: 50 (probe 2), (3) a combination of primer set 3 consisting of a primer consisting of the base sequence of SEQ ID NO: 5 and a primer consisting of base sequence of SEQ ID NO: 6, and a probe consisting of a base sequence with not less than 95% identity with the base sequence of SEQ ID NO: 51, or a probe consisting of a base sequence with not less than 95% identity with the base sequence complementary to the base sequence of SEQ ID NO: 51 (probe 3), (4) a combination of primer set 4 consisting of a primer consisting of the base sequence of SEQ ID NO: 7 and a primer consisting of the base sequence shown by SEQ ID NO: 8, and a probe consisting of a base sequence with not less than 95% identity with the base sequence of SEQ ID NO: 52, or a probe consisting of a base sequence with not less than 95% identity with the base sequence complementary to the base sequence of SEQ ID NO: 52 (probe 4), (5) a combination of primer set 5 consisting of a primer consisting of the base sequence of SEQ ID NO: 9 and a primer consisting of the base sequence of SEQ ID NO: 10, and a probe consisting of a base sequence with not less than 95% identity with the base sequence of SEQ ID NO: 53, or a probe consisting of a base sequence with not less than 95% identity with the base sequence complementary to the base sequence of SEQ ID NO: 53 (probe 5), (6) a combination of primer set 6 consisting of a primer consisting of the base sequence of SEQ ID NO: 11 and a primer consisting of the base sequence of SEQ ID NO: 12, and a probe consisting of a base sequence with not less than 95% identity with the base sequence of SEQ ID NO: 54, or a probe consisting of a base sequence with not less than 95% identity with the base sequence complementary to the base sequence of SEQ ID NO: 54 (probe 6), (7) a combination of primer set 7 consisting of a primer consisting of the base sequence of SEQ ID NO: 13 and a primer consisting of the base sequence of SEQ ID NO: 14, and a probe consisting of a base sequence with not less than 95% identity with the base sequence of SEQ ID NO: 55, or a probe consisting of a base sequence with not less than 95% identity with the base sequence complementary to the base sequence of SEQ ID NO: 55 (probe 7), (8) a combination of primer set 8 consisting of a primer consisting of the base sequence of SEQ ID NO: 15 and a primer consisting of the base sequence of SEQ ID NO: 16, and a probe consisting of a base sequence with not less than 95% identity with the base sequence of SEQ ID NO: 56, or a probe consisting of a base sequence with not less than 95% identity with the base sequence complementary to the base sequence of SEQ ID NO: 56 (probe 8), (9) a combination of primer set 9 consisting of a primer consisting of the base sequence of SEQ ID NO: 17 and a primer consisting of the base sequence of SEQ ID NO: 18, and a probe consisting of a base sequence with not less than 95% identity with the base sequence of SEQ ID NO: 57, or a probe consisting of a base sequence with not less than 95% identity with the base sequence complementary to the base sequence of SEQ ID NO: 57 (probe 9), (10) a combination of primer set 10 consisting of a primer consisting of the base sequence of SEQ ID NO: 19 and a primer consisting of the base sequence of SEQ ID NO: 20, and a probe consisting of a base sequence with not less than 95% identity with the base sequence of SEQ ID NO: 58, or a probe consisting of a base sequence with not less than 95% identity with the base sequence complementary to the base sequence of SEQ ID NO: 58 (probe 10), (11) a combination of primer set 11 consisting of a primer consisting of the base sequence of SEQ ID NO: 21 and a primer consisting of the base sequence of SEQ ID NO: 22, and a probe consisting of a base sequence with not less than 95% identity with the base sequence of SEQ ID NO: 59, or a probe consisting of a base sequence with not less than 95% identity with the base sequence complementary to the base sequence shown by SEQ ID NO: 59 (probe 11), (12) a combination of primer set 12 consisting of a primer consisting of the base sequence of SEQ ID NO: 23 and a primer consisting of the base sequence of SEQ ID NO: 24, and a probe consisting of a base sequence with not less than 95% identity with the base sequence of SEQ ID NO: 60, or a probe consisting of a base sequence with not less than 95% identity with the base sequence complementary to the base sequence of SEQ ID NO: 60 (probe 12), (13) a combination of primer set 13 consisting of a primer consisting of the base sequence of SEQ ID NO: 25 and a primer consisting of the base sequence of SEQ ID NO: 26, and a probe consisting of a base sequence with not less than 95% identity with the base sequence of SEQ ID NO: 61, or a probe consisting of a base sequence with not less than 95% identity with the base sequence complementary to the base sequence of SEQ ID NO: 61 (probe 13), (14) a combination of primer set 14 consisting of a primer consisting of the base sequence of SEQ ID NO: 27 and a primer consisting of the base sequence of SEQ ID NO: 28, and a probe consisting of a base sequence with not less than 95% identity with the base sequence of SEQ ID NO: 62, or a probe consisting of a base sequence with not less than 95% identity with the base sequence complementary to the base sequence of SEQ ID NO: 62 (probe 14), (15) a combination of primer set 15 consisting of a primer consisting of the base sequence of SEQ ID NO: 29 and a primer consisting of the base sequence of SEQ ID NO: 30, and a probe consisting of a base sequence with not less than 95% identity with the base sequence of SEQ ID NO: 63, or a probe consisting of a base sequence with not less than 95% identity with the base sequence complementary to the base sequence shown by SEQ ID NO: 63 (probe 15), (16) a combination of primer set 16 consisting of a primer consisting of the base sequence of SEQ ID NO: 31 and a primer consisting of the base sequence of SEQ ID NO: 32, and a probe consisting of a base sequence with not less than 95% identity with the base sequence of SEQ ID NO: 64, or a probe consisting of a base sequence with not less than 95% identity with the base sequence complementary to the base sequence of SEQ ID NO: 64 (probe 16), (17) a combination of primer set 17 consisting of a primer consisting of the base sequence of SEQ ID NO: 33 and a primer consisting of the base sequence of SEQ ID NO: 34, and a probe consisting of a base sequence with not less than 95% identity with the base sequence of SEQ ID NO: 65, or a probe consisting of a base sequence with not less than 95% identity with the base sequence complementary to the base sequence of SEQ ID NO: 65 (probe 17), (18) a combination of primer set 18 consisting of a primer consisting of the base sequence of SEQ ID NO: 35 and a primer consisting of the base sequence of SEQ ID NO: 36, and a probe consisting of a base sequence with not less than 95% identity with the base sequence of SEQ ID NO: 66, or a probe consisting of a base sequence with not less than 95% identity with the base sequence complementary to the base sequence of SEQ ID NO: 66 (probe 18), (19) a combination of primer set 19 consisting of a primer consisting of the base sequence of SEQ ID NO: 37 and a primer consisting of the base sequence of SEQ ID NO: 38, and a probe consisting of a base sequence with not less than 95% identity with the base sequence of SEQ ID NO: 67, or a probe consisting of a base sequence with not less than 95% identity with the base sequence complementary to the base sequence of SEQ ID NO: 67 (probe 19), (20) a combination of primer set 20 consisting of a primer consisting of the base sequence of SEQ ID NO: 39 and a primer consisting of the base sequence of SEQ ID NO: 40, and a probe consisting of a base sequence with not less than 95% identity with the base sequence of SEQ ID NO: 68, or a probe consisting of a base sequence with not less than 95% identity with the base sequence complementary to the base sequence of SEQ ID NO: 68 (probe 20), (21) a combination of primer set 21 consisting of a primer consisting of the base sequence of SEQ ID NO: 41 and a primer consisting of the base sequence of SEQ ID NO: 42, and a probe consisting of a base sequence with not less than 95% identity with the base sequence of SEQ ID NO: 69, or a probe consisting of a base sequence with not less than 95% identity with the base sequence complementary to the base sequence of SEQ ID NO: 69 (probe 21), (22) a combination of primer set 22 consisting of a primer consisting of the base sequence of SEQ ID NO: 43 and a primer consisting of the base sequence of SEQ ID NO: 44, and a probe consisting of a base sequence with not less than 95% identity with the base sequence of SEQ ID NO: 70, or a probe consisting of a base sequence with not less than 95% identity with the base sequence complementary to the base sequence of SEQ ID NO: 70 (probe 22), and (23) a combination of primer set 23 consisting of a primer consisting of the base sequence of SEQ ID NO: 45 and a primer consisting of the base sequence of SEQ ID NO: 46, and a probe consisting of a base sequence with not less than 95% identity with the base sequence of SEQ ID NO: 71, or a probe consisting of a base sequence with not less than 95% identity with the base sequence complementary to the base sequence of SEQ ID NO: 71 (probe 23).