Patent ID: 8129148
Filing Date: 2012-03-06
Classification: C12P

Abstract:
1. A method for producing a fraction comprising more than 50% of chondroitin represented formula (1), comprising the steps: (a) reacting a glucuronic acid donor, a N-acetylgalactosamine donor, a saccharide acceptor, chondroitin polymerase derived from (b) determining a reaction time for providing the chondroitin content ratio, X, as determined in step (a) in a fraction produced when the reaction is performed under conditions of arbitrary temperature and pH, other than at 30° C. and pH 7.2, at which the chondroitin polymerase derived from (c) performing the reaction at the same temperature and pH as step (b) for a time shorter than Y, wherein formulae (1) and (2) are: wherein GlcA represents a glucuronic acid residue, GalNac represents an N-acetylgalactosamine residue, - represents a glycosidic bond and n represents an arbitrary integer.