Patent ID: 8288085
Filing Date: 2012-10-16
Classification: A01N,A61K,C12N

Abstract:
1. A pancreatic islet isolation method comprising the sequential steps of: (1) injecting a protection solution containing a protease inhibitor and approximately 4 to 50 mmol/L of potassium into the pancreatic duct of a procured pancreas, wherein said protease inhibitor is at least one selected from the group consisting of ulinastatin, gabexate mesilate and nafamostat mesilate; (2) preserving said pancreas into which the protection solution has been injected by a two-layer method that comprises introducing (i) a liquid perfluorocarbon and (ii) a preservation solution containing a protease inhibitor, approximately 4 to 50 mmol/L of potassium, and 80 to 160 mmol/L of trehalose into a container and forming two layers, feeding oxygen into the container, and preserving said pancreas in said container; (3) injecting a collagenase solution into said pancreas into which the protection solution has been injected and digesting the pancreas; and (4) purifying the digested pancreatic tissue using a purification solution containing a density gradient reagent wherein said density gradient reagent is Ficoll solution or iodixanol.