Patent ID: 8685677
Filing Date: 2014-04-01
Classification: A01H,C12N,C12Q

Abstract:
1. A method for determining event zygosity of a soybean plant comprising an AAD-12 soybean event pDAB4468-0416, said event comprising a transgene construct comprising an AAD-12 gene, said transgene construct being flanked by a 5′ flanking soybean genomic DNA and a 3′ flanking soybean genomic DNA, said method comprising: obtaining a DNA sample of genomic DNA from said soybean plant; producing a contacted sample by contacting said DNA sample with subjecting said contacted sample to fluorescence-based endpoint TAQMAN PCR conditions; quantitating said florescent event probe that hybridized to said event amplicon; quantitating said florescent reference probe that hybridized to said reference amplicon; comparing amounts of hybridized florescent event probe to hybridized florescent reference probe; and determining zygosity of pDAB4468-0416 by comparing florescence ratios of hybridized fluorescent event probe and hybridized fluorescent reference probe.