Patent ID: 9063118
Filing Date: 2015-06-23
Classification: B01L,C12N,G01N

Abstract:
1. A method for purifying viable cancerous epithelial cells in an in vitro fluid preparation comprising viable cancerous epithelial cells, viable normal epithelial cells, and extracellular calcium, the method comprising: applying an initial pulse of fluid shear stress to the preparation, wherein the initial pulse of fluid shear stress induces a fluid shear stress resistance in the viable cancerous epithelial cells such that the magnitude of shear stress required to kill the viable cancerous epithelial cells is greater than the magnitude of shear stress required to kill the viable normal epithelial cells; and subsequently applying one or more additional pulses of fluid shear stress to the preparation comprising the fluid shear stress-resistant viable cancerous epithelial cells, said fluid shear stress sufficient to selectively kill the viable normal epithelial cells relative to the viable cancerous epithelial cells, whereby the ratio of viable cancerous epithelial cells to viable normal epithelial cells in the preparation is increased.