Patent ID: 6673615
Filing Date: 2004-01-06
Classification: B82Y,C07K,C12N,C12Q,G01N,Y10S

Abstract:
A method of detecting alterations or mutations in a polynucleic acid sequence, comprising:providing a surface containing a channel of a dimension sufficient to allow sequential monomer-by-monomer passage of a single-stranded polynucleic acid, but not of a double-stranded polynucleic acid; providing a source of a test polynucleic acid to be analyzed at one side of the surface; inducing passage of the test polynucleic acid through the channel and monitoring the passage of the test polynucleic acid through the channel; and wherein the method is performed at a temperature that allows a probe to hybridize to the test polynucleic acid if the probe is perfectly complementary to a portion of the polynucleic acid, but increases the probability of no hybridization occurring if the probe varies from being perfectly complementary to the test polynucleic acid.