Patent ID: 9140688
Filing Date: 2015-09-22
Classification: C12N,G01N

Abstract:
1. A method of gender enriching one or more sperm subpopulations comprising the steps of: a. obtaining a population of sperm having both X-chromosome bearing sperm and Y-chromosome bearing sperm; b. staining at least a portion of the population of sperm with a first dye that is a DNA selective fluorescent dye which fluoresces when excited; c. staining said portion of the population of sperm with a second dye that is a dead quenching dye for selectively quenching fluorescence emitted by the DNA selective fluorescent dye within membrane compromised sperm; d. staining said portion of the population of sperm with a third dye that is a split enhancing dye; and e. sorting the stained sperm into one or more gender enriched subpopulations of viable sperm.