Patent ID: 8030020
Filing Date: 2011-10-04
Classification: A61K,A61P,C12N

Abstract:
1. A process for preparing a working seed solution of a vaccine containing as an active ingredient a mutant virus that is likely to generate revertants, which comprises the following steps (1) to (3): (1) inoculating a solution of a mutant virus to 1 to n containers, wherein n is an integer, of an isolated animal cell and incubating-them; (2) diluting a portion of the cultured solution from each container and inoculating the diluted solution to RK-13 cells and to Vero cells and incubating them; and (3) selecting containers which contain a cultured solution that forms plaques in RK-13 cells but does not form plaques in Vero cells and pooling each cultured solution obtained from selected containers.