Patent ID: 8697400
Filing Date: 2014-04-15
Classification: C12Q

Abstract:
1. A method for amplification of DNA from a blood sample, the method consisting of: pretreating a blood sample having DNA to be amplified by mixing the blood sample having DNA to be amplified, with an alkaline aqueous solution under a temperature range between 15° C. and 40° C., so as to extract double-stranded DNA from the blood sample and to dissociate the double-stranded DNA into a single-stranded DNA to obtain a blood-derived sample including the single-stranded DNA; preparing an isothermal amplification reaction solution comprising a mixture of the blood-derived sample, a primer, deoxyribonucleotide triphosphate (dNTP), a strand-displacing DNA polymerase, a magnesium salt and a buffer, to establish an isothermal amplification reaction system for the strand-displacing DNA polymerase; and amplifying DNA in the isothermal amplification reaction system using the single-stranded DNA as a template.