Patent ID: 6133027
Filing Date: 2000-10-17
Classification: C12N

Abstract:
An isolated, inducible, nucleotide expression system, comprising:(a) a first nucleotide sequence comprising an inducible promoter operably linked to a sequence encoding a gene product of interest, wherein expression from the inducible promoter is regulated by a multi-chimeric transactivating factor; and(b) a second nucleotide sequence comprising a promoter operably linked to a sequence encoding a multi-chimeric transactivating factor, wherein the multi-chimeric transactivating factor comprises:(i) a first ligand-binding domain that binds a first ligand;(ii) a second ligand-binding domain that binds a second ligand, wherein the second ligand-binding domain is different from the first ligand-binding domain and the second ligand is different from the first ligand; and(iii) a eukaryotic transcriptional activation domain,wherein the gene product of interest is expressed from the inducible promoter under the following conditions:the multi-chimeric transactivating factor is expressed,first ligand is not bound to the first ligand-binding domain of the multi-chimeric transactivating factor, andsecond ligand is bound to the second ligand-binding domain of the multi-chimeric transactivating factor.