Patent ID: 6207066
Filing Date: 2001-03-27
Classification: A61K,B01D,B01J,C12N,C12Y,G01N,Y10T

Abstract:
A method for rapid purification of plasminogen from blood comprising the steps of:centrifuging blood plasma from cellular blood elements;activating an affinity cartridge of the type which binds with plasminogen, said affinity cartridge containing affinity media selected from the group consisting of silica and ceramic matrix having a solid support and a molecule disposed thereon having affinity for the plasminogen;passing the centrifued blood plasma through the affinity cartridge without prior solvent extraction of blood plasma so that the plasminogen is retained by the affinity media, the affinity media having a solid support which can withstand high pressure and a molecule disposed thereon having affinity for the plasminogen;thereafter eluting the plasminogen from the affinity cartridge by passing a releasing agent through the affinity cartridge, the releasing agent disengaging the plasminogen from the affinity cartridge; andthereafter separating the releasing agent from the solution containing both the eluted releasing agent and the plasminogen.