Patent ID: 6251635
Filing Date: 2001-06-26
Classification: C07K,C12N

Abstract:
A method for producing a calcitonin carba analog comprising condensing an N-terminal fragment of the formula: ##STR11##wherein A.sub.2 is Gly, Ser or Ala; A.sub.3 is Asn or Ser; A.sub.8 is Val or Met; R.sup.2 is --(CH.sub.2).sub.4 -- or --CH(NH.sub.2)CH.sub.2 S--S--; and Y is OH, OR.sup.1, where --R.sup.1 is a lower alkyl group;with a recombinantly-formed polypeptide of the formula:A.sub.10 -A.sub.11 -A.sub.12 -A.sub.13 -A.sub.14 -A.sub.15 -A.sub.16 -A.sub.17 -A.sub.18 -A.sub.19 -A.sub.20 -A.sub.21 -A.sub.22 -A.sub.23 -A.sub.24 -A.sub.25 -A.sub.26 -A.sub.27 -Gly-A.sub.29 -A.sub.30 -A.sub.31 -Pro-Xxx (SEQ ID NO:1)wherein A.sub.10 is Gly or Ser, A.sub.11 is Lys, Thr or Ala, A.sub.12 is Leu or Tyr, A.sub.13 is Ser, Thr or Trp, A.sub.14 is Gln, Lys or Arg, A.sub.15 is Glu, Asp or Asn, A.sub.16 is Leu or Phe, A.sub.17 is His or Asn, A.sub.18 is Lys or Asn, A.sub.19 is Leu, Tyr or Phe, A.sub.20 is Gln or His, A.sub.21 is Thr or Arg, A.sub.22 is Tyr or Phe, A.sub.23 is Pro or Ser, A.sub.24 is Arg, Gly or Gln, A.sub.25 is Thr or Met, A.sub.26 is Asp, Ala, Gly, or Asn, A.sub.27 is Val, Leu, Ile, Phe, or Thr, A.sub.29 is Ala, Val, Pro or Ser, A.sub.30 is Gly, Val or Glu, A.sub.31 is Thr, Val or Ala, and -Xxx is --OH, --NH.sub.2, an amino acid residue or a polypeptide group;in the presence of a non-enzymatic coupling reagent to form a calcitonin-derivative having the formula: ##STR12## A.sub.13 -A.sub.14 -A.sub.15 -A.sub.16 -A.sub.17 -A.sub.18 -A.sub.19 -A.sub.20 -A.sub.21 -A.sub.22 -A.sub.23 -A.sub.24 -A.sub.25 -A.sub.26 -A.sub.27 -Gly-A.sub.29 -A.sub.30 -A.sub.31 -Pro-Xxx (SEQ ID NO:48).