Patent ID: 8372600
Filing Date: 2013-02-12
Classification: G01N

Abstract:
1. A method of measuring change in cell volume comprising: introducing cells into a first chamber defined by a first pair of electrodes for measuring voltage drop across said first chamber, wherein a first chamber volume of said first chamber is between 2 and 100 times a cell volume of said cells; introducing a first electrically conductive extracellular fluid into said first chamber; applying a current through said first chamber; measuring voltage drop across said first chamber to obtain a first voltage drop result corresponding to said first electrically conductive extracellular fluid; exchanging the first electrically conductive extracellular fluid in said first chamber with a second electrically conductive extracellular fluid; applying a current through said first chamber; measuring voltage drop across said first chamber to obtain a second voltage drop result corresponding to said second electrically conductive extracellular fluid; using said first voltage drop result and said second voltage drop result in conjunction with known voltage drops across said first chamber for the first and second electrically conductive extracellular fluids, respectively, absent impedance to current flow attributable to said cells, to monitor changes in said cell volume, said changes in said cell volume are calculated according to: wherein V