Patent ID: 6733986
Filing Date: 2004-05-11
Classification: C07C,C12Q,G01N

Abstract:
Method for detecting and identifying and/or quantifying a deaminase enzymatic activity of a microorganism, comprising:bringing an inoculum suspected of containing a microorganism with a deaminase activity into contact with a culture medium for microorganisms, and detecting and identifying and/or quantifying a deaminase enzymatic activity; wherein the culture medium comprises at least one detection agent for demonstrating a deaminase activity by forming a colored product with a revealing agent comprising a color or fluorescent indicator; said detection agent being an L-amino acid of following general formula (I): â€ƒin which R represents an organic radical containing a cyclic ring, said cyclic ring being substituted with 1 to 3 substituents that are identical or different and each of which limits the diffusion in the culture medium of the &agr;-keto acid produced by the deamination of the at least one detection agent, as compared to where each of said substituents is not present.