Patent ID: 7968337
Filing Date: 2011-06-28
Classification: A61K,A61P,C12N

Abstract:
1. A method for the generation of an isolated, non-tumorigenic cell composition consisting essentially of embryonic stem cell-derived neural precursor cells, and neuronal cells derived from the embryonic stem cell-derived neural precursor cells comprising: (a) culturing mouse or human embryonic stem cells that are not human genetically modified embryonic stem cells to produce neural precursor cells; (b) culturing the neural precursor cells from (a) in a first growth factor-containing serum-free medium, the first medium comprising basic fibroblast growth factor (bFGF); (c) culturing the cells from (b) in a second growth factor-containing serum-free medium, the second medium comprising bFGF and epidermal growth factor (EGF); and (d) culturing the cells from (c) in a third growth factor-containing serum-free medium, the third medium comprising bFGF and platelet-derived growth factor (PDGF), to obtain the cell composition consisting essentially of embryonic stem cell-derived neural precursor cells, and neuronal cells derived from the embryonic stem cell-derived neural precursor cells.