Patent ID: 6133033
Filing Date: 2000-10-17
Classification: C12N

Abstract:
Potato plants of the Lemhi Russet variety regenerated in accordance with a method for in vitro selection of blackspot resistant tubers from regenerated Lemhi Russet potato plants obtained from tissue culture, said method comprising the steps of:(a) culturing tissue obtained from said potato plants in cell layer medium and associated reservoir medium;(b) subculturing said tissue on callus proliferation medium to obtain callus formation;(c) subculturing said callus on shoot induction medium to obtain shoot formation;(d) subculturing said shoot on a rooting medium to ensure root formation, whereby potato plants are regenerated from which blackspot resistant tubers are produced; and(e) adding at least one melanin precursor to at least one of said reservoir, callus proliferation, and rooting media, whereby said potato plants are regenerated from the calli and roots which show no blackening response when the melanin precursor is added.