Patent ID: 6251601
Filing Date: 2001-06-26
Classification: B01J,C12Q,C40B

Abstract:
A method for simultaneous detection of gene expression and chromosomal abnormality in a tissue sample comprising:(a) providing an array of nucleic acid target elements attached to a solid support wherein the nucleic acid target elements comprise polynucleotide sequences substantially complementary under preselected hybridization conditions to nucleic acids indicative of gene expression and indicative of chromosomal sequence of a tissue sample;(b) providing at least three labeled nucleic acid populations:(i) a mRNA or cDNA population labeled with a first marker and derived from the tissue sample,(ii) a chromosomal DNA population labeled with a second marker and derived from the tissue sample, and(iii) at least one reference nucleic acid population labeled with a third marker;(c) contacting the array with the labeled nucleic acid populations under hybridization conditions; and(d) detecting presence and intensity of each of the first, second and third markers on at least two target elements.