Patent ID: 6861230
Filing Date: 2005-03-01
Classification: C12Q

Abstract:
1. A method for determining the susceptibility of a bacteria to a reagent selected from an antibiotic agent or a biostatic agent or a compound suspected of having an antibiotic property or a compound suspected of having a biostatic property, said method comprising the steps of: (i) dividing a culture comprising said bacteria into at least a first sample and a second sample; (ii) incubating said first sample in the presence of said reagent to form a first incubated sample, lysing bacteria in said first incubated sample to form a first lysed-incubated sample, exposing the first lysed-incubated sample to ADP, a source of magnesium ions, luciferin and luciferase, to form a first mixture, and measuring luminescence emitted from said first mixture as an indication of the amount of any adenylate kinase present in the first lysed-incubated sample; (iii) subjecting said second sample to either of the following steps (iii)(a) and (iii)(b); (iv) comparing the indicated amount of adenylate kinase present in the first lysed-incubated sample with the indicated amount of any adenylate kinase present in the second lysed-incubated sample or the first lysed sample as a determination of the susceptibility of said bacteria to the reagent, wherein