Patent ID: 8598409
Filing Date: 2013-12-03
Classification: C07K,C12N

Abstract:
1. A transgenic, non-human organism comprising a gene expression modulation system comprising: a) a first gene expression cassette that is capable of being expressed in said organism comprising a polynucleotide sequence that encodes a first hybrid polypeptide comprising: i) a DNA-binding domain that recognizes a response element associated with a gene whose expression is to be modulated; and ii) an ecdysone receptor ligand binding domain; and b) a second gene expression cassette that is capable of being expressed in said organism comprising a polynucleotide sequence that encodes a second hybrid polypeptide comprising: i) a transactivation domain; and ii) a chimeric retinoid X receptor ligand binding domain comprising a vertebrate retinoid X receptor amino acid sequence and an invertebrate retinoid X receptor amino acid sequence, wherein at least one alpha helix of said vertebrate retinoid X receptor ligand binding domain is replaced with the same number of alpha helices from an invertebrate retinoid X receptor ligand binding domain or the same number of alpha helices from an ultraspiracle (USP) receptor ligand binding domain, wherein the genome of said non-human organism comprises said gene expression modulation system, and wherein said system modulates the expression of said gene.