Patent ID: 7659373
Filing Date: 2010-02-09
Classification: A61K,A61P,C07K

Abstract:
1. A process for manufacturing an erythropoietin (EPO) composition consisting essentially of glycosylated EPO molecules which contain, on average, at least 4.3 N-acetyl-lactosamine units per N-linked carbohydrate chain per EPO molecule in said composition, or an average of at least 13.0 N-acetyl-lactosamine units with reference to total N-glycosylation of an EPO molecule in said composition, comprising culturing a cell capable of producing said glycosylated EPO molecules, under conditions favoring production thereof, said conditions comprising a temperature of 35° C., wherein said composition contains a higher amount of EPO isoforms IF2, IF3, and IF4 than a composition produced by culturing said cells at 36.5° C.