Patent ID: 8512971
Filing Date: 2013-08-20
Classification: A61P,C07F,C12N,C12Q,G01N

Abstract:
1. A method for assaying the lysophospholipase D (LysoPLD) activity of autotoxin (ATX) comprising the steps of: a) obtaining a solution having ATX present; b) adding a LysoPLD substrate comprising a lysophospholipid derivative labeled with a fluorescent group and a quencher group to said solution containing ATX, wherein said fluorescent group and quencher group are both active in the near infrared spectrum; and c) determining if LysoPLD activity is present in said solution by measuring the change in the fluorescent signal in said solution that is generated by the cleavage of said lysophospholipid derivative represented by the formula: wherein A represents O or S; B represents H, Na and the quencher is