Patent ID: 6649749
Filing Date: 2003-11-18
Classification: C12Q,Y10S

Abstract:
An oligonucleotide for use in determining the presence of HIV Type 1 nucleic acid in a sample, said oligonucleotide being up to 100 nucleotide bases in length and comprising an at least 10 contiguous nucleoride base sequence consisting of or contained within a sequence selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 55, SEQ ID NO: 57, SEQ ID NO: 58, SEQ ID NO: 59, SEQ ID NO: 60, SEQ ID NO: 65, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 72, SEQ ID NO: 73, SEQ ID NO: 74, SEQ ID NO: 79, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 86, SEQ ID NO: 87, SEQ ID NO: 88, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 132, SEQ ID NO: 133, SEQ ID NO: 134, SEQ ID NO: 135, SEQ ID NO: 136, SEQ ID NO: 137, SEQ ID NO: 138 and SEQ ID NO: 139, provided that if said oligonucleotide comprises an at least 10 contiguous nucleotide base sequence consisting of or contained within the sequence of SEQ ID NO: 3, SEQ ID NO: 55, SEQ ID NO: 69 or SEQ ID NO: 83, then said oligonucleotide further comprises a detectable moiety, wherein said oligonucleotide hybridizes to an HIV Type 1 target nucleic acid sequence in a sample to form an oligonucleotide:target duplex under hybridization conditions, and wherein said oligonucleotide does not hybridize to human nucleic acid in said sample to form an oligonucleotide:non-target duplex under said conditions.