Patent ID: 9175064
Filing Date: 2015-11-03
Classification: A01K,C07K

Abstract:
1. An HLA-E chimeric molecule that when expressed in a nonhuman mammal cell, is expressed at the cell surface and that possesses one of the following amino acid sequences: (1) an HLA-E chimeric molecule wherein the signal peptide (SP) of an HLA-E molecule has been replaced with a reformed SP, wherein the sequence of the reformed SP is SEQ ID NO:21, and the serine corresponding to amino acid 57 of SEQ ID NO: 3 of the α2 domain of the HLA-E molecule has been replaced with cysteine, (2) an HLA-E chimeric molecule wherein the signal peptide (SP) of an HLA-E molecule has been replaced with a reformed SP, wherein the sequence of the reformed SP is SEQ ID NO:21, the serine corresponding to amino acid 11 of SEQ ID NO:2 of the α1 domain of the HLA-E molecule has been replaced with alanine; and the serine corresponding to amino acid 57 of SEQ ID NO: 3 of the α2 domain of the HLA-E molecule has been replaced with cysteine, and (3) an HLA-E chimeric molecule wherein the signal peptide (SP) of an HLA-E molecule has been replaced with a reformed SP, wherein the sequence of the reformed SP is SEQ ID NO: 21, and the first portion of the latter part of the α2 domain of the HLA-E chimeric molecule, corresponding to amino acids 47-60 of SEQ ID NO: 3 has been replaced with the first portion of the latter part of the α2 domain of the HLA-G1 molecule, corresponding to amino acids 47-60 of SEQ ID NO: 13, wherein the amino acid sequences of the signal peptide (SP), α1 domain, α2 domain, α3 domain and transmembrane domain of the HLA-E molecule are SEQ ID NO: 1-5, respectively.