Patent ID: 7294509
Filing Date: 2007-11-13
Classification: C12N

Abstract:
1. An isolated culture of an immortalized human pre-adipose cell line, wherein the immortalized human pre-adipose cell are genetically engineered to express a human papillomavirus E7 oncoprotein and a telomerase reverse transcriptase gene, wherein the immortalized human pre-adipose cell is capable of maturing into an immortalized mature white adipose cell, wherein the mature adipose cell exhibits a metabolic pattern of normal human white adipose cells when cultured on serum-free medium, wherein the adipose cell expresses at least four normal human white adipose cell markers selected from the group consisting of: lipoprotein lipase; fatty acid synthase; adipocyte fatty acid binding protein; leptin; adipsin; ad iponectin; peroxisome proliferator-activated receptors γ; peroxisome proliferator-activated receptors β; CCAAT/enhancer binding protein alpha; and hormone sensitive lipase.