Patent ID: 8906617
Filing Date: 2014-12-09
Classification: C12Q

Abstract:
1. A method for sequencing comprising: immobilizing a single stranded nucleic acid target by its 3′ end or 5′ end to a surface of a substrate, the single-stranded nucleic acid target including a plurality of tandem repeat nucleic acid sequences, the single-stranded nucleic acid target including a detectable moiety at its free end; applying at least one oligonucleotide complementary to at least a portion of a tandem repeat single stranded nucleic acid sequence of the nucleic acid target, the at least one oligonucleotide having a known sequence and hybridizing to the at least a portion of the tandem repeat nucleic acid sequence of the single stranded nucleic acid target; applying a force to the single stranded nucleic acid target; determining with an ion sensitive field effect transistor array and when the force is applied, a change in a length of the single stranded nucleic acid target arising from hybridizing the at least one oligonucleotide, the determining based on a change in a position of the detectable moiety caused by the conversion of single stranded bases to double stranded bases; and associating the change in the length of the single stranded nucleic acid target with a nucleotide sequence for the nucleic acid target.