Patent ID: 6107024
Filing Date: 2000-08-22
Classification: C07C,C07D,C07F,C07H,C12Q,G01N,Y10S,Y10T

Abstract:
In an enzyme-linked assay method wherein the enzyme is linked to another substance, selected from an antigen, antibody, hapten and nucleic acid, is reacted with a substrate, the improvement which comprises using a triggerable 1,2-dioxetane as the substrate wherein the 1,2-dioxetane is of the formula ##STR33## wherein ArOX represents an aryl group substituted with an X-oxy group which forms an aryloxide intermediate 1,2-dioxetane compound when triggered by removing X with an activating agent comprising said enzyme so that the aryloxide intermediate 1,2-dioxetane decomposes and releases electronic energy to form light and two carbonyl containing compounds of the formula ##STR34## wherein X is a chemical labile group which is removed by the activating agent to form the unstable oxide intermediate 1,2-dioxetane and wherein A are passive organic groups which allow the light to be produced by the 1,2-dioxetane when X is removed.