Patent ID: 6060312
Filing Date: 2000-05-09
Classification: C12N

Abstract:
A method for in vitro selection of blackspot resistant tubers from regenerated potato plants obtained from tissue culture, comprising the steps of:(a) culturing tissue obtained from a potato plant in cell layer medium and associated reservoir medium;(b) subculturing said tissue on callus proliferation medium to obtain callus formation;(c) subculturing said callus on shoot induction medium to obtain shoot formation;(d) subculturing said shoot on a rooting medium to ensure root formation, whereby potato plants are regenerated from which blackspot resistant tubers are produced; an(e) adding at least one melanin precursor to at least one of said reservoir, callus proliferation, and rooting media, whereby said potato plants are regenerated from the calli and roots which show no blackening response when the melanin precursor is added.