Patent ID: 6663861
Filing Date: 2003-12-16
Classification: A61K,C07K

Abstract:
A method for producing antibodies against molecules in a host animal comprising:(a) producing a modified carrier protein from unmodified carrier protein wherein the first portion of said unmodified carrier protein is chemically modified to carry a net positive charge and the second portion is chemically modified to carry a net negative charge; (b) coupling a molecule to said modified carrier proteins obtained in step (a) to form a complex; (c) forming a balanced charge mixture comprising complexes obtained in step (b); (d) pre-immunizing a host animal with an unmodified carrier protein; (e) administering to the host animal the balanced charge mixture obtained in step (c) so that antibodies are produced against the molecule; and (f) isolating the antibodies or B cells producing antibodies against the molecule from the host animal.