Patent ID: 7518033
Filing Date: 2009-04-14
Classification: A01K,C12N,G01N

Abstract:
1. A method of producing a genetically modified mammalian cell, said method comprising the steps of: (a) inserting into one or more mammalian cells an artificial chromosome comprising a cassette which includes a first region of homology having at least 90% sequence identity to a first region of an endogenous chromosome of said one or more mammalian cells, a selectable marker, and a second region of homology having at least 90% sequence identity to a second region of said endogenous chromosome under conditions that result in homologous recombination between said artificial chromosome and said endogenous chromosome and integration of said cassette into said endogenous chromosome of one or more mammalian cells; and (b) selecting a mammalian cell in which said homologous recombination occurs, thereby selecting a genetically modified mammalian cell.