Patent ID: 8367325
Filing Date: 2013-02-05
Classification: C07H,C12Q

Abstract:
1. An oligonucleotide set comprising a pair of polymerase chain reaction (PCR) primers in a single buffer for amplifying a selected DNA amplification target sequence, said target sequence and primers defining a double-stranded amplification sequence bracketed by the primer pair, said amplification sequence having a melting temperature (T m A ), wherein T m A =81.5+0.41(% G+% C)−500/L+16.6 log 0.07/(1+0.7(0.07)), where L is the length in nucleotides, comprising a limiting primer and an excess primer, wherein the ratio (X) of limiting primer to excess primer is not more than 0.05, wherein X=(concentration of limiting primer)/(concentration of excess primer); wherein the limiting primer has a melting temperature (T