Patent ID: 8703446
Filing Date: 2014-04-22
Classification: C07K,C12N,C12P

Abstract:
1. A method for producing an L-amino acid comprising cultivating an L-amino acid-producing bacterium of genus Escherichia in a culture medium, and collecting said L-amino acid from the culture medium, wherein said bacterium has been modified to enhance the expression of a bssR gene as compared to a non-modified bacterium, wherein said bssR gene comprises: i) the nucleotide sequence of SEQ ID NO:1, or ii) a nucleotide sequence which a) hybridizes with the complete complement of the nucleotide sequence of SEQ ID NO:1 under stringent conditions comprising washing at a salt concentration of 0.1×SSC, 0.1% SDS at 60° C., and b) encodes a protein which has all of the same activities as a protein consisting of the amino acid sequence of SEQ ID NO:2; wherein said expression is enhanced by a method selected from the group consisting of: a) replacing the native promoter of the bssR gene with a more potent promoter, b) increasing the DNA copy number of the bssR gene per cell, and c) combinations thereof.