Patent ID: 8452546
Filing Date: 2013-05-28
Classification: G01N,G16B

Abstract:
1. A method for deducing a most likely sequence of monomers of a region of a polymer from measurements that are made nominally in sequential order, but for which an arrival time and/or an order of the monomers is subject to stochastic variations, the method comprising: driving one or more polymers into a nanopore; recording two or more observations of the same region of the one or more polymers, wherein the observations are a series of measurements of predetermined duration; allocating a discrete monomer identity to each apparent monomer occurrence in the two or more observations to produce a trial sequence of monomers, which could produce a complete set of observations, for each of the two or more observations; quantifying a probability that each trial sequence of monomers could produce the complete set of observations, based on known statistics of a sensing process utilized in recording the two or more observations of the same region of the one or more polymers; and selecting the trial sequence of monomers with the highest total probability as the most likely sequence of monomers of the region of the one or more polymers.