Patent ID: 8956870
Filing Date: 2015-02-17
Classification: C12N

Abstract:
1. An in vitro method for generating a lineage specific precursor/progenitor cell population (LSP) comprising: (a) obtaining a population of isolated immature antigen-presenting cells; (b) activating said population of isolated immature antigen-presenting cells to generate a population of activated antigen-presenting cells; (c) obtaining a population of cells comprising stem/progenitor cells and enriching for stem/progenitor cells within said population of cells to generate an enriched population of stem/progenitor cells; and (d) following the activating, combining the population of activated antigen-presenting cells of step (b) with the enriched population of stem/progenitor cells of step (c) to form a combined population, wherein said activated antigen-presenting cells are 2-20% of said combined population when said combined population is formed, and wherein said activated antigen-presenting cells induce at least one process in the enriched population of stem/progenitor cells of the combined population, wherein the process is selected from the group consisting of: differentiation, expansion, activation, secretion of a molecule, and expression of a marker, thereby generating a lineage specific precursor/progenitor population (LSP) in vitro from the enriched population of stem/progenitor cells, wherein at least one of the isolated immature antigen-presenting cells of step (a) or the population of cells comprising stem/progenitor cells of step (c) is obtained from umbilical cord blood.