Patent ID: 7883707
Filing Date: 2011-02-08
Classification: A61K,A61P

Abstract:
1. A method of decreasing an antibody response to an administered soluble therapeutic polypeptide in a mammalian host, comprising the steps of: (a) administering to the mammalian host for at least about two weeks effective amounts of (i) cyclosporine A and (ii) azathioprine, wherein the cyclosporine A is maintained at a trough blood level of at least 200 ng/ml, and wherein the azathioprine is administered at a dose of at least 2.5 mg/kg/day; (b) after step (a), administering to the mammalian host for at least about two weeks effective amounts of (i) cyclosporine A, (ii) azathioprine, and (iii) a soluble form of the therapeutic polypeptide that naturally comprises or is conjugated to a mannose-6-phosphate high uptake moiety, wherein the cyclosporine A is maintained at a trough blood level of at least 200 ng/ml, and wherein the azathioprine is administered at a dose of at least 2.5 mg/kg/day; (c) after step (b), discontinuing administration of cyclosporine A and azathioprine; and (d) after step (c), administering the soluble therapeutic polypeptide, wherein during step (d) the mammalian host exhibits an antibody titer to the soluble therapeutic polypeptide in the absence of continued immune suppression that is decreased compared to the antibody titer induced by the soluble therapeutic polypeptide in a mammalian host that has not undergone steps (a)-(c).