Patent ID: 7383135
Filing Date: 2008-06-03
Classification: C07K,C12Q,G01N,G16B

Abstract:
1. A method for identifying an inhibitor of an unphosphorylated JNK3α (c-Jun N-terminal kinase 3α) molecule, comprising the steps of: a) producing a crystal of an unphosphorylated JNK3α (c-Jun N-terminal kinase 3α) molecule comprising amino acid residues 40-402 of SEQ ID NO:1 and a chemical entity; b) determining the three-dimensional atomic coordinates of amino acids Ile70, Gly71, Ser72, Gly73, Ala74, Gln75, Gly76, Val78, Ala91, Lys93, Glu111, Ile124, Met146, Glu147, Leu148, Met149, Asp150, Ala151, Asn152, Gln155, Lys191, Ser193, Asn194, Val196 and Leu206 of an active site binding pocket of the unphosphorylated JNK3α molecule by X-ray diffraction of the crystal; c) using the atomic coordinates of Ile70, Gly71, Ser72, Gly73, Ala74, Gln75, Gly76, Val78, Ala91, Lys93, Glu111, Ile124, Met146, Glu147, Leu148, Met149, Asp150, Ala151, Asn152, Gln155, Lys191, Ser193, Asn194, Val196 and Leu206 according to d) employing said three-dimensional structure to design or select a potential inhibitor; e) synthesizing said potential inhibitor; and f) contacting said potential inhibitor with said molecule to determine the ability of said potential inhibitor to associate with said molecule.