Patent ID: 6492160
Filing Date: 2002-12-10
Classification: B01J,C07K,C12N,C40B,G01N

Abstract:
Recombinant vectors produced by a method which comprises:causing or allowing recombination between (a) first vectors comprising nucleic acid encoding a population of first polypeptide chain of a specific binding pair member; and (b) second vectors comprising nucleic acid encoding a population of a second polypeptide chain of a specific binding pair member, at least one of said populations being genetically diverse, the recombination resulting in recombinant vectors each of which comprises nucleic acid encoding a said first polypeptide chain and a said second polypeptide chain, the recombination being promoted by inclusion in said first and second vectors of sequences at which site-specific recombination occurs, each of the first vectors and each of the second vectors including a first site-specific recombination sequence and a second site-specific recombination sequence different from the first, site-specific recombination taking place preferentially between first site-specific recombination sequences on different vectors and between second site-specific recombination sequences on different vectors compared with a first site-specific recombination sequence and a second site-specific recombination sequence on the same vector.