Patent ID: 6900023
Filing Date: 2005-05-31
Classification: G01N,Y10S,Y10T

Abstract:
1. A method for classifying and counting leukocytes comprising the steps of: ( (2) removing erythrocytes from the hematological sample; (3) analyzing the resulting hematological sample using a flow cytometer to measure at least one scattered light signal and three separate fluorescence signals; (4) classifying granulocytic cells on the basis of intensity of the scattered light and intensity of fluorescence from the first fluorescence-labeled antibody; (5) distinguishing eosinophils and neutrophilic cells in the granulocytic cells obtained in step (4) on the basis of the intensity of the fluorescence from the first fluorescence-labeled antibody and the intensity of the fluorescence from the second or third fluorescence-labeled antibody; (6) classifying the neutrophilic cells obtained in step (5) into groups different in degree of maturity on the basis of the intensity of the fluorescence from the second fluorescence-labeled antibody and the intensity of the fluorescence from the third fluorescence-labeled antibody; and (7) counting the number of cells in each of the groups.