Patent ID: 8921055
Filing Date: 2014-12-30
Classification: G01N

Abstract:
1. A method for labeling a cell producing an antigen-specific antibody, the method comprising: obtaining a cell sample containing multiple antibody-producing cells; tagging the antibody-producing cells with a cross-linking reagent, wherein the cross-linking reagent has a first portion configured to bind to a cell surface marker that is specific to the antibody-producing cells and a second portion configured to bind to an antigen of interest; exposing the antibody-producing cells to the antigen of interest such that the antibody-producing cells become linked through the cross-linking reagent to the antigen of interest; allowing the antibody-producing cells to produce antibodies, such that a portion of the antibody-producing cells produce the antigen-specific antibody, which binds to the antigen of interest; and exposing the antibody-producing cells to a labeled secondary antibody configured to bind to the antigen-specific antibody.