Patent ID: 6107045
Filing Date: 2000-08-22
Classification: C07K,G01N,Y10S

Abstract:
A method for determining the concentration of a specific lipoprotein, an apolipoprotein, or lipid associated with a specific lipoprotein, in a biological sample comprising:contacting the sample with antibody molecules immunoreactive with a specific lipoprotein or apolipoprotein, wherein the antibody molecules are selected from the group consisting of monoclonal antibodies, recombinant antibodies, and monoclonal antibody fragments that specifically bind to a stable, conformation independent epitope which is uninfluenced by the lipid content of the apoliprotein and lipoprotein molecule specifically bound by the antibody, wherein the molecule is selected from the group consisting of lipoproteins and apolipoproteins;under conditions wherein the antibody molecules can form an immune complex with lipoprotein or apolipoprotein in the sample; anddetermining the amount of lipoprotein, apolipoprotein, or lipid associated with a lipoprotein bound by the antibody molecules by detecting the amount of immune complex formed by binding of the antibody molecules with the lipoprotein or apolipoprotein in the sample.