Patent ID: 7387887
Filing Date: 2008-06-17
Classification: C09B,C12Q,Y10S

Abstract:
1. A PCR reaction mixture comprising a. a target nucleic acid, b. PCR reagents, c. a pair of oligonucleotide primers configured for amplifying a portion of the target nucleic acid to produce an amplicon, and d. a dsDNA binding dye having the formula: wherein the moiety Y represents an optionally-substituted fused monocyclic or polycyclic aromatic ring or an optionally-substituted fused monocyclic or polycyclic nitrogen-containing heteroaromatic ring; X is oxygen, sulfur, selenium, tellurium or a moiety selected from C(CH R t=0 or 1; Z is a charge selected from 0 or 1; R n=0,1, or 2; and Q is an heterocycle selected from the group of structures consisting of: wherein R R wherein the dye is selected from the group consisting of F7, N7, O7, P7, Q7, R7, S7, T7, V7, W7, X7, T8, A9, C9, I9, J9, K9, L9, M9, N9, O9, P9 and R9 as presented in Table 2.