Patent ID: 6709859
Filing Date: 2004-03-23
Classification: C12N,C12P,G03G

Abstract:
A recombinant vector comprising an isolated DNA (I) segment having a nucleotide sequence endogenous to Streptomyces clavuligerus ATCC 27064 or a restricted fragment derived therefrom, comprising one or more genes coding for one or more enzymes directly involved in the biosynthesis of penicillin and cephalosporin &bgr;-lactams, said enzyme selected from the group consisting of penicillin N synthetase, deacetoxy cephalosporin C synthetase, and O-carbamoyldesacetylcephalosporin C-7-hydroxylase, said DNA (I) having the configuration of restriction sites shown in FIG. 1(a) cloned into a plasmid derived from a virulent phage.