Patent ID: 8877514
Filing Date: 2014-11-04
Classification: G01N,Y10S

Abstract:
1. A method for identifying the presence of an antigen or an antibody in a sample from a human subject, the method comprising: contacting the sample with a complementary capture antibody or a capture antigen in an aqueous solution, specifically binding the antigen or the antibody to the complementary capture antibody or capture antigen, respectively; and detecting the presence of the antigen or antibody bound to the complementary capture antibody or capture antigen with a detector antibody that binds to either (a) the antigen, which is bound to the complementary capture antibody, or (b) the antibody, which is bound to the capture antigen, said sample further comprising a human anti-animal antibody that interferes with the specific binding between the antigen or the antibody in the sample and the complementary capture antibody or capture antigen, said aqueous solution comprising: wherein the ratio of the non-ionic detergent to the compound A is from 1:15 to 1:25; wherein the non-ionic detergent is a compound selected from the group consisting of: a) a substituted phenyl residue having substituents R b) c) Dodecylpoly(ethyleneglycolether) d) 1-O-n-Octyl-β-D-glucopyranodise (n-octylglucoside); and e) 1-O-n-Dodecyl-β-D-glucopyranosyl (1-4)alpha-D-glucopyranoside; and wherein said aqueous solution reduces the binding of the human anti-animal antibody in the sample to the complementary capture antigen or capture antibody, thereby reducing interference by the human anti-animal antibody with the specific binding of said antigen or antibody to the complementary capture antibody or capture antigen, compared to conducting said specific binding in the absence of said aqueous solution.