Patent ID: 7524634
Filing Date: 2009-04-28
Classification: C07K,C12N,C12Q,G01N

Abstract:
1. A method which is prognostic for a gastric preneoplastic/neoplastic disease afflicting a subject vertebrate, said method comprising: (a) detecting MN/CA IX protein or MN/CA IX polypeptide in a gastric preneoplastic/neoplastic tissue sample comprising neoplastic cells taken from said vertebrate, (b) quantitating the level of said MN/CA IX protein or MN/CA IX polypeptide in said sample, (c) comparing the level of MN/CA IX protein or MN/CA IX polypeptide of step (b) to the average level of MN/CA IX protein or MN/CA IX polypeptide in comparable gastric preneoplastic/neoplastic tissue samples comprising neoplastic cells taken from vertebrates also afflicted by a gastric preneoplastic/neoplastic disease, wherein said vertebrates are of the same species as the subject vertebrate, and (d) determining that said subject vertebrate has a prognosis of shorter survival if the level of MN/CA IX protein or MN/CA IX polypeptide of step (b) is higher than the average level of MN/CA IX protein or MN/CA IX polypeptide in said comparable gastric preneoplastic/neoplastic tissue samples, than if said MN/CA IX protein or MN/CA IX polypeptide of step (b) were absent or at a significantly reduced level in said sample relative to said average level; wherein said MN/CA IX protein is encoded by a nucleotide sequence selected from the group consisting of: (1) SEQ ID NO: 1's coding region; (2) nucleotide sequences that hybridize under stringent hybridization conditions of 50% formamide at 42 degree C. to complement of SEQ ID NO: 1's coding region; and (3) nucleotide sequences that differ from SEQ ID NO: 1's coding region or from the nucleotide sequences of (2) in codon sequence due to the degeneracy of the genetic code; wherein said MN/CA IX protein or said MN/CA IX polypeptide is specifically bound by the M75 monoclonal antibody that is secreted from the hybridoma VU-M75, which was deposited at the American Type Culture Collection under ATCC No. HB 11128.