Patent ID: 8415153
Filing Date: 2013-04-09
Classification: C12N

Abstract:
1. A method of enriching the proportion of islet-like cells from a cell population generated by the differentiation of human pluripotent stem cells to islet-like cells, comprising: a) differentiating human pluripotent stem cells into a cell population comprising islet-like cells by a method comprising, i. contacting human pluripotent stem cells with a first medium comprising Activin A; and ii. contacting the cells from step i) with a second medium comprising FGF-2 but lacking Activin A, b) separating at least some of the buds from at least some of the clusters in the cell population of step (a) into at least two fractions, wherein the fraction containing separated buds contains a higher proportion of insulin or glucagon-producing islet-like cells than the cell population of step (a) and wherein the buds are separated from the clusters based on the size difference between the buds and the clusters.