Patent ID: 9060507
Filing Date: 2015-06-23
Classification: A01N,A61P

Abstract:
1. A sterile aqueous solution for gravity microperfusion of donor hearts, the solution consisting of: (a) between 10 and 20 mM potassium chloride; (b) between 5 and 10 mM magnesium; (c) between 0.2 and 1.0 mM calcium; (d) between 10 and 40 mM Tris(hydroxymethyl)aminomethane hydrochloride (Tris or THAM), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HE PES), 3-(Nmorpholino)propanesulfonic acid (MOPS), 2-(N-morpholino)ethanesulfonic acid (MES), N,N-bis-(2-hydroxyethyl)-2-aminoethansulfonic acid (BES), or Ntris(hydroxymethyl)methyl-2-aminoethanesulfonic acid (TES); (e) between 10 and 30 mM sodium bicarbonate, for enhancing the out-flow of CO (f) between 1 and 40 mM aspartate; (g) between 1 and 30 mM glucose; (h) between 1 and 20 mM adenosine, cAMP or cGMP; (i) between 30 and 100 mM lactobionate; (j) between 1 and 20 units/L insulin; (k) between 1 and 10 mM fructose diphosphate or a salt thereof; (l) between 1 and 10 mM reduced glutathione; (m) oxygen saturation using 100% 02; and (n) a sufficient concentration of sodium to maintain an ionic strength of sodium in said sterile aqueous solution is to be between 110 and 120 mM.