Patent ID: 7291474
Filing Date: 2007-11-06
Classification: C12Q,G01N,Y10S

Abstract:
1. In an assay for detecting or quantitating an analyte, wherein in the operation of said assay, said analyte reacts to form a specific binding pair, which is localized on a solid phase support, said assay employing an analyte-dependent enzyme activation system in which the localization of the specific binding pair on the solid phase support localizes an enzyme on said solid phase support, and wherein said localized enzyme reacts with a substrate portion of a conjugate, said conjugate comprising a detectably labeled substrate for said enzyme, so as to form an activated conjugate, which activated conjugate covalently binds to a receptor site for said activated conjugate on said solid phase support proximate where said specific binding pair is localized, said receptor not being reactive with the unactivated conjugate of the analyte-dependent enzyme activation system, wherein the detectably labeled portion of the bound conjugate either directly or indirectly generates a signal which is detected or quantitated, the improvement comprising: using as said conjugate, a compound having a structure selected from the group consisting of: wherein Y is a moiety capable of being cleaved by a hydrolytic enzyme; L is a detectable label; X is a group linking L to the phenyl group; Z is a halogen; and R is selected from hydrogen, halogen and alkyl groups.