Patent ID: 9125731
Filing Date: 2015-09-08
Classification: A61F

Abstract:
1. A method for preparing stromal/endothelial cell tissue of a donor eye for endothelial keroplasty using a microkeratome, the method comprising: mounting a corneal section of the donor eye in a chamber of an artificial anterior chamber (AAC) device such that the corneal section is exposed via an opening of the AAC device; mounting a microkeratome in the opening such that the corneal section is accessible by a cutting head of the microkeratome; placing a fluid pressure source in communication with the chamber in a closed loop system by interconnecting a first fluid conduit between the fluid pressure source and the chamber; placing a pressure monitoring gauge in communication with the chamber by interconnecting a second fluid conduit between the pressure monitoring gauge and the first fluid conduit, at a location of the first fluid conduit between the fluid pressure source and the AAC device; applying pressure to the corneal section by operating the fluid pressure source communicating with the chamber via the first fluid conduit; cutting stromal/endothelial cell tissue from the corneal section by operating the microkeratome; while cutting, monitoring the pressure applied to the corneal section by operating the pressure monitoring gauge in the closed loop system, wherein the pressure monitoring gauge displays an indication of the pressure being applied; and based on the pressure indication displayed, adjusting the fluid pressure source to maintain the applied pressure within the AAC device within a range of about 75 to 125 mmHg.