Patent ID: 9096868
Filing Date: 2015-08-04
Classification: A61K,A61P,C07K,C12N

Abstract:
1. A recombinant replication defective hepatitis B virus (HBV) comprising: a hepatitis B virus genome defective for replication due to a deletion in the capsid-encoding portion of the virus, wherein the virus contains a polyadenylation signal for HBV mRNA, a polymerase reading frame, and a nucleotide sequence of up to about 195 nucleotides encoding at least one heterologous immuno-dominant epitope of a pathogenic bacteria or virus fused within the N-terminal part of the capsid-encoding portion of the virus between the polyadenylation signal and the start of the polymerase reading frame; wherein following infection by the virus of an isolated vertebrate hepatocyte comprising a nucleotide sequence encoding HBc for complementation of the recombinant hepatitis virus, the nucleotide sequence encoding the immuno-dominant epitope of a pathogenic bacteria or virus is expressed and the immuno-dominant epitope of a pathogenic bacteria or virus is presented at the surface of the vertebrate hepatocyte as a T cell target.