Patent ID: 9096883
Filing Date: 2015-08-04
Classification: C12M,C12Q,G01N

Abstract:
1. A method for detecting a gas-producing microorganism, comprising the steps of: providing an automated detection system, a sample suspected of containing a gas-producing microorganism; a flat film culture device comprising a cover sheet that optionally includes a dry medium layer adhered thereto, a self-supporting substrate having a cold-water-reconstitutable dry medium adhered thereto, and a culture medium containing a fermentable nutrient; and a membrane filter which is substantially transparent when in contact with a hydrated culture medium in the culture device, the filter having a first major surface and a second major surface opposite the first major surface; collecting a gas-producing microorganism from the sample onto the first major surface of the filter; placing at least one major surface of the filter with the microorganisms collected thereon into contact with the reconstitutable dry medium; hydrating the reconstitutable dry medium and the optional dry medium layer, if present, with a predetermined volume of aqueous liquid before or after placing the filter into contact with the dry medium in order to form a semi-solid growth medium; closing the culture device to bring the cover sheet or the optional medium layer, if present, into contact with a major surface of the filter opposite the at least one major surface of the filter; incubating the closed culture device for a period of time; and detecting the presence of a gas-producing microorganism; wherein detecting the presence of the gas-producing microorganism comprises detecting the gas-producing microorganism with the automated detection system; wherein detecting the presence of the gas-producing microorganism comprises detecting a colony; wherein the colony is detected optically; and wherein detecting the presence of the gas-producing microorganism further comprises detecting a gas bubble proximate to the colony.