Patent ID: 7202024
Filing Date: 2007-04-10
Classification: G01N

Abstract:
1. A method for identifying a compound that modulates apoptosis, the method comprising: a. providing a cell containing an expression vector comprising a bbc3 gene promoter operably linked to either a polynucleotide encoding bbc3 or a polynucleotide encoding a heterologous reporter protein, b. exposing said cell to a candidate compound, and c. determining expression of said polynucleotide encoding Bbc3 or said polynucleotide encoding a heterologous reporter protein, wherein a change in expression is an indication that said candidate compound modulates apoptosis, and wherein said bbc3 gene promoter is a polynucleotide selected from the group consisting of (a) a polynucleotide comprising nucleotides 1–2099 of SEQ ID NO: 1, (b) a polynucleotide comprising nucleotides 1168–2099 of SEQ ID NO: 1, (c) a polynucleotide comprising nucleotides 1934–2099 of SEQ ID NO: 1, (d) a polynucleotide comprising a polynucleotide sequence having at least about 95% homology to bases 1–2099 of SEQ ID NO: 1, (e) a polynucleotide comprising a polynucleotide sequence having at least about 95% homology to bases 1168–2099 of SEQ ID NO:1, and (f) a polynucleotide comprising a polynucleotide sequence having at least about 95% homology to bases 1934–2099 of SEQ ID NO:1, wherein each of said nolynucleotides (a)–(f) has promoter activity for said polynucleotide encoding a heterologous reporter protein.