Patent ID: 6242257
Filing Date: 2001-06-05
Classification: A01H,C12N

Abstract:
A method for regenerating viable and fertile cotton plants by tissue culture from an apical explant of a germinated cotton seedling, said method comprising the steps of:(i) cutting from the germinated cotton seedling the apical explant, said explant consisting essentially of a hypocotyl and an apical meristem,(ii) culturing the explant for the purpose of shoot proliferation in a first medium that is free of auxin and sucrose, at a temperature between 20 to 40.degree. C. in light of at least 40 .mu.mol/m.sup.2 s for a 16 hour photoperiod, said first medium having a pH in the range of 5.4 to 6.2, being sterile as a result of autoclaving and comprising:a. salts,b. vitamins,c. glucose,d. cytokinins at a concentration between 0.1 to 100 .mu.M, ande. a gelling agent;(iii) continuing culturing of the explant for a minimum period of 4 weeks until shoots are formed;(iv) harvesting the shoots by removing said shoots from a basal mass of regenerating tissue; and(v) culturing each harvested shoot in a second medium at a temperature between 20 to 40.degree. C. in light of at least 40 .mu.mol/m.sup.2 s for a 16 hour photoperiod until roots are formed, said second medium having a pH in the range of 5.4 to 6.2, being sterile as a result of autoclaving, and comprising:a. salts,b. vitamins,c. a carbon source,d. auxins at a concentration between 0 to 50 .mu.M, ande. a gelling agent;wherein viable and fertile cotton plants are obtained.