Patent ID: 7244819
Filing Date: 2007-07-17
Classification: A61K,A61P,C07K,C12N,G01N

Abstract:
1. An isolated inclusion body, comprising a fusion polypeptide encoded by a recombinant DNA molecule comprising: a first nucleotide sequence coding for a target polypeptide; and a second nucleotide sequence coding for an FKBP chaperone located upstream to the first nucleotide sequence, wherein the FKBP chaperone is FkpA or SlyD, wherein the FkpA chaperone comprises amino acids 26-270 of a wild-type FkpA chaperone, wherein the SlyD chaperone comprises a C-terminally truncated wild-type SlyD chaperone that provides for a functional chaperone activity, and wherein the FKBP chaperone allows for a cytosolic expression and refolding of the fusion polypeptide.