Patent ID: 6777233
Filing Date: 2004-08-17
Classification: A61K,A61P,C12N,G01N

Abstract:
A cell culture comprising:(a) a culture medium containing one or more predetermined growth factors effective for inducing multipotent central nervous system (CNS) neural stem cell proliferation; and (b) suspended in the culture medium, human multipotent CNS neural stem cells wherein (i) the cells are grown in culture medium containing one or more predetermined growth factors effective for inducing multipotent CNS neural stem cell proliferation; (ii) the population comprises cells which stain positive for nestin; (iii) in the presence of differentiation-inducing conditions, the cells produce progeny cells that differentiate into neurons, astrocytes, or oligodendrocytes; and (iv) the cells have a doubling rate faster than 30 days.