Patent ID: 8940505
Filing Date: 2015-01-27
Classification: C07K,C12N,C12P

Abstract:
1. A composition comprising a Yarrowia lipolytica -derived cell population (Accession No. NCIMB 42022, deposited on 9 Aug. 2012 at National Collection of Industrial Food and Marine Bacteria (NCIMB Ltd), of Ferguson Building, Craibstone Estate, Bucksburn, Aberdeen, AB21 9YA, Scotland) capable of expressing a heterologous polypeptide of interest, wherein the level of extracellular secretion of the heterologous polypeptide is increased relative to the level of extracellular secretion of the heterologous polypeptide by a control Yarrowia lipolytica cell population (Accession No. NCIMB 42021, deposited on 9 Aug. 2012 at National Collection of Industrial Food and Marine Bacteria (NCIMB Ltd), of Ferguson Building, Craibstone Estate, Bucksburn, Aberdeen, AB21 9YA, Scotland), wherein the Yarrowia lipolytica -derived cell population has at least one genomic modification affecting one or more genes involved in glycoslyphosphatidylinositol (GPI) anchoring relative to the control Yarrowia lipolytica cell population, wherein the genomic modification is attributable to the increase in extracellular secretion.