Patent ID: 6245509
Filing Date: 2001-06-12
Classification: C12Q

Abstract:
A method for obtaining an oligonucleotide probe that is hybridizable under predetermined conditions to a nucleotide sequence contained by one or more target organisms but not by one or more selected non-target organisms in a sample, wherein said target and non-target organisms are no higher phylogenetically than prokaryotes, the method comprising:a) obtaining particular nucleotide sequence information of one or more of said target organisms;b) obtaining particular nucleotide sequence information of one or more of said selected non-target organisms;c) comparing said target and non-target sequence information and identifying therefrom at least one oligonucleotide sequence that is hybridizable under said predetermined conditions to a nucleotide sequence from said one or more target organisms but not to a nucleotide sequence from said one or more selected non-target organisms; andd) synthesizing and isolating an oligonucleotide comprising a sequence identical to said identified sequence.