Patent ID: 8354510
Filing Date: 2013-01-15
Classification: B01D,B01J,C07K,Y10S

Abstract:
1. In a method of separating one or more antibodies from a liquid, which method comprises: (a) contacting said liquid with a separation matrix comprising ligands immobilised to a support; (b) allowing antibodies to adsorb to said matrix by interaction with said ligands; (c) optionally washing the adsorbed antibodies; and (d) recovering antibodies by contacting said matrix with an eluent which releases the antibodies; the improvement being a mutated protein A-based ligand having a N23T mutation in the B-domain of wild-type Staph protein A defined by SEQ ID N:1 or in protein Z defined by SEQ ID NO: 2 and further comprises a second mutation at position 43 of SEQ ID NO:2, which protein confers an increased chemical stability at alkaline pH-value.