Patent ID: 6444428
Filing Date: 2002-09-03
Classification: C12N

Abstract:
A method of enhancing a nucleic acid polymerase reaction comprising, in any appropriate order:(a) mixing a nucleic acid sequence template for a nucleic acid polymerase with at least one nucleic acid polymerase; and (b) adding to the polymerase reaction medium a polymerase enhancing, non-naturally occurring composition of matter comprising at least one component possessing nucleic acid polymerase enhancing activity selected from an isolated or purified naturally-occurring polymerase enhancing protein obtained from a bacterial or archaeal source; a wholly or partially synthetic protein having the same amino acid sequence as said naturally-occurring protein or analogs thereof possessing polymerase enhancing activity; polymerase-enhancing mixtures of one or more of said naturally occurring or wholly or partially synthetic proteins; polymerase-enhancing protein complexes of one or more of said naturally occurring or wholly or partially synthetic proteins; or polymerase enhancing. partially purified cell extracts containing one or more of said naturally occurring proteins.