Patent ID: 6589799
Filing Date: 2003-07-08
Classification: C12N

Abstract:
A method of producing a derivatized matrix material with aldehyde groups suitable for immobilizing bio-molecules, said method comprising,a) providing a raw matrix material, said raw matrix material having a surface area and hydroxyl groups occurring on said surface area; b) forming a suspension of said raw matrix material in an organic solvent; c) activating said hydroxyl groups by adding an aqueous acid to said suspension, and equilibrating said suspension for a period of at least about 15 minutes; d) adding to said suspension in small aliquots over a period of at least about 1 hour, an alkoxy aldehydic silane to produce a derivatized matrix material, wherein said alkoxy aldehydic silane has a structure selected from the group: â€ƒwherein, R1 is alkyl from C1 to C30, phenyl, naphthyl or a covalent bond, and R2 and R3 are independently alkyl or alkenyl from C1 to C30, phenyl, naphthyl or silyl, and R4 alkyl or alkenyl from C1 to C30, phenyl or naphthyl, and â€ƒwherein, R1 is alkyl from C1 to C30, phenyl, naphthyl or a covalent bond, and R2, R3 and R4 are independently alkyl or alkenyl from C1 to C30, phenyl, naphthyl or silyl, and; e) washing said derivatized matrix material with a solvent.