Patent ID: 7416847
Filing Date: 2008-08-26
Classification: C07K,C12N

Abstract:
1. A method of producing a peptide or protein expression library in vitro which displays a population of peptides or proteins, wherein the peptides or proteins are specifically associated with the DNA encoding them through covalent binding of the peptides or proteins to the encoding DNA, said method comprising at least the following steps: 1) preparing a genetic library of a population of DNA molecules, each DNA molecule comprising: (a) a nucleotide sequence encoding a binding moiety comprising an amino acid sequence which is a cis-acting DNA binding protein which binds specifically to the DNA encoding sequence through covalent binding of the amino acid sequence to DNA, and (b) a nucleotide sequence encoding a display moiety comprising an amino acid sequence for display, and wherein the display moiety comprises at least one site of attachment for the binding moiety, and 2) expressing the genetic library thus formed whereby the population of peptides or proteins is produced each specifically associated with the DNA encoding sequence through covalent binding.