Patent ID: 9096900
Filing Date: 2015-08-04
Classification: C12Q

Abstract:
1. A method for identifying CD56-expressing natural killer cells in a sample derived from a human, wherein said method comprises the steps of: a) obtaining a sample comprising immune cells from said human, b) performing a nucleic acid based assay on the cells in the sample to determine the methylation status of at least one region of a GNLY gene, wherein the methylation status of the at least one region is determined by a method comprising amplifying the at least one region using a primer pair of SEQ ID NO: 146 and 147 and bisulfite sequencing, and c) identifying an immune cell in said sample as a CD56-expressing natural killer cell if the CpG positions in said at least one region as amplified are at least 90% demethylated as determined in step b).