Patent ID: 8975216
Filing Date: 2015-03-10
Classification: B01J,B82Y,C12Q,Y10T

Abstract:
1. A method of producing a substrate having an enzyme selectively immobilized in preselected regions thereon, comprising: providing a substrate having a plurality of nanoscale wells defined thereon and having photoactivatable chemical functional groups immobilized on a surface of the substrate, wherein each of the nanoscale wells limits an ability of light to provide active chemical groups on selected portions of the surface of the substrate, wherein the nanoscale wells substantially prevent portions of the substrate surface other than the selected portions from being exposed to the light, wherein the selected portions are bottom surfaces of zero mode waveguides; exposing the substrate to the light to selectively provide active chemical groups on the selected portions of the surface of the substrate, wherein the light is selected to activate the photoactivatable chemical functional groups; and coupling the enzyme to the active chemical groups or to an additional group which is coupled to the active chemical groups, thereby coupling the enzyme to the selected portions of the surface of the substrate; wherein density of the enzyme coupled to the selected portions of the surface of the substrate is 100 times or more greater than density of the enzyme on the portions of the substrate surface other than the selected portions.