Patent ID: 7118854
Filing Date: 2006-10-10
Classification: C12N,C12Q,G01N

Abstract:
1. A method for identifying a compound that modulates PAK2-mediated T lymphocyte activation, the method comprising the steps of: (i) contacting a host T cell comprising a PAK2 polypeptide having serine/threonine kinase activity with the compound, the PAK2 polypeptide encoded by a nucleic acid that hybridizes under stringent conditions to the complement of the nucleic acid encoding a polypeptide having an amino acid sequence of SEQ ID NO:2, wherein the stringent conditions comprise incubating at 42° C. in a solution comprising 50% formamide, 5x SSC, and 1% SDS and washing at 65° C. in a solution comprising 0.2×SSC and 0.1% SDS; (ii) activating the host T cell via a T cell receptor (TCR-CD3) and determining whether the compound modulates the TCR-CD3 signaling cascade in the host T cell; and (iii) determining whether the compound modulates PAK2 activity, whereby modulation of PAK2 activity and modulation of TCR-CD3 signaling cascade indicates the compound modulates PAK2-mediated T lymphocyte activation.