Patent ID: 6436717
Filing Date: 2002-08-20
Classification: G01N,Y10T

Abstract:
A method for optical chemical sensing of an analyte in a medium, said method comprising the steps of:a) exposing a dye solution to the analyte; b) illuminating said dye solution to induce a first output light from said dye solution corresponding to a first wavelength and a second output light from said dye solution corresponding to a second wavelength; c) measuring an intensity of said first output light to determine a first spectral property â€œX1â€  corresponding to said first wavelength; d) measuring an intensity of said second output light to determine a second spectral property â€œX2â€  corresponding to said second wavelength, said first and second spectral properties being selected from the group consisting of absorbances, fluorescent emission intensities and normalized fluorescent emission intensities; and e) determining an analyte content â€œYanalyteâ€  in the medium by means of an expression of the form: Yanalyte=f&af;(k1&it;k2&it;X1-k3&it;X2X2-k4&it;X1),â€ƒwhere â€œfâ€  is a function; and â€œk1,â€  â€œk2,â€  â€œk3â€  and â€œk4â€  are constants.