Patent ID: 6264941
Filing Date: 2001-07-24
Classification: A61K,C07K,C12N

Abstract:
An implantable biocompatible immunoisolatory capsule for providing long-term, stable expression of a biologically active molecule wherein the biologically active molecule is provided to the aqueous humor or vitreous humor of the eye of a mammalian host, the capsule comprising:(a) a core comprising one or more cells dispersed in a biocompatible matrix, the cell or cells being transfected with a recombinant DNA molecule comprising(i) a DNA sequence coding for the biologically active molecule(ii) operatively linked to a promoter not subject to down regulation upon implantation in the host; and(b) an external diffuisional surface jacket surrounding the core, wherein the jacket has a molecular weight cutoff below the molecular weight of substances essential for immunological rejection of the cells, but permitting therethrough, passage of substances between the eye of the mammalian host and the core,wherein the capsule is implanted into the aqueous humor or vitreous humor of the eye of the mammalian host, and wherein the capsule provides 1-1500 ng/day of the biologically active molecule to the mammalian host.