Patent ID: 8969532
Filing Date: 2015-03-03
Classification: A61K,A61P,C07K,C12P,Y10S

Abstract:
1. A method of making a composition comprising a first polypeptide conjugate, said first polypeptide conjugate comprising a first number of poly(alkylene oxide) moieties covalently linked to said first polypeptide, wherein at least one of said poly(alkylene oxide) moieties is covalently linked to said first polypeptide via a glycosyl linking group, wherein said glycosyl linking group is either covalently linked to an amino acid residue of said first polypeptide or is covalently linked to a glycosyl moiety of said first polypeptide, said method comprising: (a) contacting a mixture comprising said first polypeptide conjugate with an anion exchange medium; (b) eluting said first polypeptide conjugate from said anion exchange medium, forming a first eluate comprising said first polypeptide conjugate; (c) contacting said first eluate with a hydrophobic interaction chromatography (HIC) medium; and (d) eluting said first polypeptide conjugate from said hydrophobic interaction chromatography medium, thereby making said composition comprising said first polypeptide conjugate.