Patent ID: 6309646
Filing Date: 2001-10-30
Classification: A61K,A61P,C07K,Y10S

Abstract:
A method for producing a soluble conjugate vaccine that is capable of inducing an immune response to a polysaccharide, comprising an immunogenic conjugate and a pharmaceutically acceptable medium or delivery vehicle produced by the steps of:(a) producing the immunogenic conjugate by derivatizing a polysaccharide with a nucleophilic group selected from thiols, amines, and hydrazides reacting the derivatized polysaccharide with a vinylsulfone group to produce a vinylsulfone derivatized polysaccharide, andreacting the vinylsulfone derivatized polysaccharide with a reactant material selected from a protein, a peptide, and a hapten, wherein said vinylsulfone derivatized polysaccharide converts to a T-cell dependent antigen upon conjugation with said reactant material; and(b) combining the immunogenic conjugate with a pharmaceutically acceptable medium or delivery vehicle to form a soluble conjugate vaccine.