Patent ID: 6242221
Filing Date: 2001-06-05
Classification: C12N

Abstract:
An isolated plant polyphenol oxidase (PPO) gene fragment consisting of a nucleotide sequence selected from the group consisting of SEO ID NOs: 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 46, 49, 51, 53, and 55 wherein said nucleotide sequence encodes a plant PPO polypeptide fragment that includes one or more conserved copper-binding sites of plant PPO polypeptides, and wherein said PPO gene fragment is prepared by a process comprising:(i) providing:(a) a tissue sample from a plant;(b) a first primer comprising a nucleotide sequence selected from the group consisting of SEQ ID NOs: 57, 58, 59, 60, 61, and 62; and(c) a second primer comprising a nucleotide sequence selected from the group consisting of SEQ ID NOs: 63, 64, 65, and 66;(ii) isolating genomic DNA from said tissue sample;(iii) performing a polymerase chain reaction on said genomic DNA using the first primer and the second primer to amplify said PPO gene fragment from said genomic DNA; and(iv) isolating the amplified PPO gene fragment.