Patent ID: 7314708
Filing Date: 2008-01-01
Classification: B01J,B01L,B82Y,C07B,C07H,C07K,C12Q,C40B,G11C,H01L

Abstract:
1. An electronic controlled method for combinatorial synthesis of a biopolymer, comprising the steps of: forming a plurality of reaction locations on a substrate, each reaction location being individually electronically addressable; disposing blocking groups upon each reaction location; placing said reaction locations in contact with a solution containing charged deblocking groups; selectively biasing those location at which reaction A is to occur at an opposite charge to the charged deblocking group, and biasing those locations at which no reaction A is to occur the same charge as the charged deblocking group, whereby the blocking group is removed from those locations at which reaction A is to occur; reacting monomer A on the deblocked specific A locations, the monomer A further including a blocking group; removing solution containing unreacted monomer A; placing said reaction locations in contact with a solution containing charged deblocking groups; selectively biasing those locations for which reaction B is to occur at the opposite charge of the charged deblocking groups, and biasing those locations at which no reaction B is to occur the same charge as the charged deblocking group, whereby the blocking groups are removed from those locations at which reaction B is to occur; reacting monomer B on the deblocked specific B locations, the monomer B further including a blocking group; and repeating the process with monomer-A, monomer-B, to monomer-N, for n-number of times until all biopolymer sequences are complete.