Patent ID: 8673562
Filing Date: 2014-03-18
Classification: C07H,C07K,C12Q,G01N,Y10S

Abstract:
1. A method of determining nucleotide sequences of one or more target polynucleotides of an organism without cell-based cloning, the method comprising the steps of: providing a plurality of aliquots, most of which contain a mixture of fragments of the target polynucleotide(s), wherein any given segment of the target polynucleotide(s) that is 100 to 300 nucleotides in length is represented by a single non-overlapping fragment in 60% or more of the aliquots containing such segment; obtaining sequence reads for fragments from the plurality of aliquots; determining the aliquot of origin of a plurality of fragments from which sequence reads have been obtained; and assembling the sequence reads to produce an assembled sequence.