Patent ID: 8759010
Filing Date: 2014-06-24
Classification: A61P,C12N,C12Q,G01N

Abstract:
1. A method for identifying a potential HCV anti-viral agent comprising steps of: (a) contacting in vitro a biological system that expresses at least one human cellular protein kinase with a candidate compound, wherein the protein kinase is selected from the group consisting of EGFR, EPHA2, FGR, AURKB, BRAF, EPHA3, EPHB1, FGFR4, ERBB4, LTK and BMP2K; and (b) determining the activity of said protein kinase, (c) identifying the candidate compound as a potential HCV anti-viral agent if the activity determined in step (b) is lower than the activity of said protein kinase determined in the biological system in the absence of the candidate compound, and (d) if the candidate compound is identified as a potential HCV anti-viral agent, incubating hepatocytes with the candidate compound, and assessing one or more of HCV entry in the incubated hepatocytes and HCV infection of the incubated hepatocytes.