Patent ID: 8668978
Filing Date: 2014-03-11
Classification: G01N,Y10T

Abstract:
1. A nanosubstrate comprising: a substrate layer comprising a photoresist material; a conductive layer deposited on the substrate layer; an insulating layer deposited on the conductive layer, the insulating layer interrupted by two or more nanotrenches or nanowells having different widths, the widths being at least about 20 nm; and one or more nanoparticles deposited in each of the two or more nanotrenches or nanowells, the nanoparticles belonging to two or more different size classes, wherein the nanoparticles in each size class have a diameter less than or equal to the width of the nanotrench or nanowell in which they are deposited, and wherein the nanoparticles of each size class are covalently bound to a different antibody or antigen binding fragment thereof.