Patent ID: 8563804
Filing Date: 2013-10-22
Classification: A01K,Y02A

Abstract:
1. A method of breeding a second hybrid (F2) generation which is a male amphidiploid fish having fertile XXXY sex chromosomes and a hybrid between a female fish (A) and a male fish (B) comprising: producing the F2 generation which is the male amphidiploid fish having fertile AxAxBxBy sex chromosomes, said F2 generation produced by fertilizing an egg AxBx with a sperm AxBy, both produced by a first hybrid (F1) generation which are an amphidiploid female fish having AxAxBxBx sex chromosomes and an amphidiploid male fish having AxAxByBy sex chromosomes, said F1 generation produced by suppressing a second cleavage of a cross-breeding egg AxBx and AxBy cross breeding a diploid female fish having the genome AxAx with a diploid male fish having the genome BxBy, wherein sperm of the male F2 amphidiploid fish is either XX or XY, thereby the third hybrid (F3) generation and subsequent generations having males and females by a ratio of 1:1.