Patent ID: 7713709
Filing Date: 2010-05-11
Classification: G01N

Abstract:
1. A method for detecting lysosomal enzyme (LE)-specific antibodies in a human comprising the steps of: (a) contacting a body fluid sample from the human with a first lysosomal enzyme labeled with a capture moiety and a second lysosomal enzyme labeled with a detection moiety to form a LE-specific antibody/lysosomal enzyme complex, (b) capturing the LE-specific antibody/lysosomal enzyme from step (a) by contacting the LE-specific antibody/lysosomal enzyme complex with a solid support that specifically binds to the capture moiety, and (c) detecting the presence of captured LE-specific antibody from the body fluid sample by detecting the presence of the second lysosomal enzyme labeled with the detection moiety.