Patent ID: 7432058
Filing Date: 2008-10-07
Classification: C07D,C07H,C09B,C12Q

Abstract:
1. A method of forming a fluorescently labeled nucleic acid, comprising the step of enzymatically extending a primer in the presence of a template nucleic acid and nucleotide triphosphates, wherein the primer comprises an energy transfer dye which comprises: (i) a xanthene donor dye capable of absorbing light at a first wavelength and emitting excitation energy in response thereto; (ii) a 4,7-dichlororhodamine acceptor dye capable of absorbing the excitation energy emitted by the donor dye and fluorescing at a second wavelength in response thereto; and (iii) a non-nucleosidic linker linking the xanthene donor dye to the 4,7-dichlororhodamine acceptor dye.