Patent ID: 8258105
Filing Date: 2012-09-04
Classification: A61K,A61P,C07H,C12N

Abstract:
1. A method comprising: identifying an animal in which reduction of a preselected target RNA in a kidney cell is desired; administering to said animal an antisense oligonucleotide optimized for kidney targeting comprising 8 to 80 linked nucleosides and that is substantially complementary to said preselected target RNA, wherein said antisense oligonucleotide comprises a central region consisting of at least 5 contiguous 2′-deoxy nucleosides flanked by a 5′ region and a 3′ region, wherein said 5′ region and said 3′ region each independently consists of at least one 2′-O-methoxyethyl nucleoside, and wherein the internucleoside linkages of the central region are phosphorothioate linkages and the internucleoside linkages of the 3′ region and of the 5′ region are phosphodiester linkages; and thereby reducing the amount of said preselected target RNA in the kidney cell in said animal.