Patent ID: 9115350
Filing Date: 2015-08-25
Classification: A61K,A61P,C07K,C12N,C12Y,Y02A

Abstract:
1. A transport protein comprising a modified Clostridium botulinum serotype B neurotoxin Hcc domain that binds to a nerve cell with a higher or lower affinity than the native Clostridium botulinum serotype B neurotoxin from which said Hcc domain is derived, said transport protein comprises substitution by a non-polar amino acid of at least one amino acid in the amino acid positions selected from the group consisting of: amino acid positions corresponding to aspartate 1114, serine 1116, proline 1117, valine 1118, tyrosine 1183, phenylalanine 1186, lysine 1188, glutamate 1191, lysine 1192, leucine 1193, phenylalanine 1194, phenylalanine 1204, phenylalanine 1243, glutamate 1245, lysine 1254, aspartate 1255 and tyrosine 1256 of Clostridium botulinum serotype B neurotoxin of SEQ ID NO.: 6, 7, 8, 9, 10 or 11, wherein the transport protein binds to synaptotagmin I or II, and wherein the transport protein is covalently linked to a Clostridium botulinum serotype B protease.