Patent ID: 7754698
Filing Date: 2010-07-13
Classification: C12N

Abstract:
1. A compound comprising a modified oligonucleotide consisting of 12 to 50 linked nucleosides targeted to a nucleic acid molecule encoding FR-alpha, wherein said modified oligonucleotide has a nucleobase sequence comprising at least 8 consecutive nucleotides complementary within nucleotides 964-1030 of SEQ ID NO:2, wherein said modified oligonucleotide has a nucleobase sequence comprising at least 8 consecutive nucleobases of a nucleobase sequence selected from the group consisting of SEQ ID NO: 33, 34, 35, 36, and 37, wherein said nucleobase sequence of said modified oligonucleotide is at least 90% complementary to SEQ ID NO:2 as measured over the entirety of said nucleobase sequence of said modified oligonucleotide, wherein the modified oligonucleotide comprises: a gap segment consisting of linked deoxynucleosides; a 5′ wing segment consisting of linked nucleosides; a 3′ wing segment consisting of linked nucleosides; wherein the gap segment is positioned between the 5′ wing segment and the 3′ wing segment, wherein each nucleoside of each wing segment comprises a modified sugar, and wherein said compound inhibits the expression of FR-alpha.