Patent ID: 8775146
Filing Date: 2014-07-08
Classification: C12N,C12Q,C12Y,G01N

Abstract:
1. A method of selecting or designing a α-N-acetylglucosaminidase (NAGLU) polypeptide binding compound, the method comprising: a) displaying the atomic coordinates of amino acids 134-658 as set forth in Table 3.1, Table 3.2, or Table 3.3 to form a three-dimensional structure of a first NAGLU polypeptide using a programmed computer; b) constructing a model of a second NAGLU polypeptide using the three-dimensional structure of the first NAGLU polypeptide as a template, wherein the second NAGLU polypeptide differs from the first NAGLU polypeptide in that the second NAGLU polypeptide has at least one amino acid substitution, deletion or duplication not present in the first NAGLU polypeptide; c) screening a binding compound that binds to the second NAGLU polypeptide in vitro, or in vivo for particular characteristics or desired properties; and d) selecting or designing the binding compound with particular characteristics or desired properties.