Patent ID: 7094540
Filing Date: 2006-08-22
Classification: C12Q

Abstract:
1. A method for determining a concentration of a target nucleic acid, which comprises hybridizing a nucleic acid probe which is labeled with only a fluorescent dye to said target nucleic acid, wherein only said nucleic acid probe is provided for one type of target nucleic acid, wherein said probe is labeled at an end portion thereof with said fluorescent dye, and said probe has a base sequence designed such that, when said probe is hybridized with said target nucleic acid, at least one G (guanine) base exists in a base sequence of said target nucleic acid at a position 1 to 3 bases apart from an end base portion where said probe and said target nucleic acid are hybridized with each other, wherein said fluorescent dye is reduced in fluorescence emission when said probe is hybridized with said target nucleic acid; and measuring a decrease in fluorescence emission from said fluorescent dye after said hybridization relative to fluorescence emission from said fluorescent dye before said hybridization.