Patent ID: 6502448
Filing Date: 2003-01-07
Classification: G01N,Y10T

Abstract:
A chromatography detection method employing an injection valve with at least one loop and a flow-through emissions detector, for handling the flow from a chromatography column, comprising the steps of:loading said at least one loop with an independent sample having a known activity and including a standard quantity of an isotope diluted with mobile phase as employed in said chromatography column, said independent sample being derived from an independent source independently of said chromatography column in a first mode without connecting said at least one loop to either said flow-through detector or said chromatography column; serially connecting said at least one loop between said chromatography column and said flow-through emissions detector in a second mode for unloading said independent sample; causing said independent sample to flow by driving a flow from said at least one loop to said detector with eluate from said chromatography column; detecting emissions in said second mode as said independent sample flows through said flow-through emissions detector; determining efficiency of said emissions detector by calculating the efficiency of detected emissions with respect to the known activity of said independent sample based on the flow of said independent sample through said emissions detector; detecting emissions when said independent sample is absent from said emissions detector and eluate from said chromatography column is present in said emissions detector; and correcting emissions readings for flow from said chromatography column using the efficiency determined from the flow of said independent sample through said emissions detector.