Patent ID: 8911945
Filing Date: 2014-12-16
Classification: C12Q

Abstract:
1. A method for genotyping genetic markers in one or more samples, comprising the steps of: (a) reducing complexity of at least two nucleic acid samples using amplified fragment length polymorphism (AFLP) to produce at least two reproducible subsets of amplified adaptor-ligated restriction fragments from the at least two nucleic acid samples, wherein each reproducible subset comprises a unique identifier tag; (b) sequencing a pool of the at least two reproducible subsets of amplified adaptor-ligated restriction fragments using sequencing by synthesis on a solid support; (c) aligning the sequences of the reproducible subsets of amplified adaptor-ligated restriction fragments; (d) identifying AFLP and/or single-nucleotide polymorphism (SNP) markers within a selection of amplified adaptor-ligated restriction fragments that are sequenced with a redundancy of at least 6; and (e) determining dominant or co-dominant genotypes of the genetic markers in the selected amplified adaptor-ligated restriction fragments that are sequenced with the redundancy of at least 6.