Patent ID: 7867744
Filing Date: 2011-01-11
Classification: C11D,C12N,C12P,C12Y,Y02E

Abstract:
1. An isolated polypeptide having cellobiohydrolase II activity, selected from the group consisting of: (a) a polypeptide having an amino acid sequence comprising the amino acid sequence shown as amino acids 1 to 82 of SEQ ID NO:4, and a polypeptide having an amino acid sequence comprising at least 85% identity with the amino acid sequence shown as amino acids 1 to 420 of SEQ ID NO: 4; (b) a polypeptide having an amino acid sequence which is encoded by nucleotides 1 to 246 of SEQ ID NO: 3 and nucleotides 1 to 1272 of SEQ ID NO: 3; (c) a polypeptide which is encoded by a nucleotide sequence which hybridizes under high stringency conditions with a polynucleotide probe selected from the group consisting of: (i) the full complementary strand of the nucleotides selected from the group consisting of: nucleotides 1 to 246 of SEQ ID NO: 3, and nucleotides 1 to 1272 of SEQ ID NO: 3; and (ii) the full complementary strand of the nucleotides selected from the group consisting of: nucleotides 1 to 200 of SEQ ID NO: 3, and nucleotides 1 to 1272 of SEQ ID NO: 3; and (d) a fragment of (a), (b) or (c) that has cellobiohydrolase II activity, wherein said high stringency hybridization of the nucleotide sequence is at 42° C. in 5×SSPE, 1.0% SDS, 5×Denhardt's solution, 100 micro gram/ml denatured salmon sperm DNA, and followed by washing three times each for 15 minutes using 0.1×SSC, 0.1% SDS at 60° C.