Patent ID: 6475372
Filing Date: 2002-11-05
Classification: G01N

Abstract:
A method for determining the hematocrit corrected concentration of an analyte in a physiological sample, said method comprising:(a) introducing said physiological sample into an electrochemical cell comprising: (i) spaced apart working and reference electrodes; and (ii) a redox reagent system comprising an enzyme and a mediator; (b) applying a first electric potential having a first polarity to said reaction cell and measuring cell current as a function of time to obtain a first time-current transient; (c) applying a second electric potential having a second polarity to said cell, wherein said second polarity is opposite said first polarity, and measuring cell current as a function of time to obtain a second time-current transient; and (d) deriving a preliminary analyte concentration from said first and second time-current transients; and (e) multiplying said preliminary analyte concentration less a background value by a hematocrit correction factor to derive said hematocrit corrected analyte concentration in said sample; whereby the hematocrit corrected concentration of said analyte in said sample is determined.