Patent ID: 8241885
Filing Date: 2012-08-14
Classification: C12N,C12Q,G01N

Abstract:
1. An isolated subtilisin variant comprising subtilisin that is altered to bind to a fusion protein that comprises a protein of interest covalently bound by a peptide bond to the C-terminus of a subtilisin prodomain protein, wherein said subtilisin variant comprises a mutation at amino acid position 32 of SEQ ID NO:3, wherein the mutation at position 32 is selected from the group of amino acid substitutions consisting of D32A, D32S, D32V, D32G, and D32T, and the subtilisin variant cleaves the peptide bond between the protein of interest and the prodomain protein of the fusion protein upon incubation of the fusion protein with the subtilisin variant in the presence of a chemical trigger comprising anions.