Patent ID: 6613516
Filing Date: 2003-09-02
Classification: C12N,C12Q,Y10S

Abstract:
A method of preparing labeled fragments of a population of nucleic acids of interest comprising:increasing the relative percentage of said population of nucleic acids of interest within a mixed population of nucleic acids, wherein said mixed population comprises a plurality of nucleic acid sequences, comprising: (a) contacting a nucleic acid sample with a nucleic acid bait molecule, wherein said nucleic acid sample comprises said nucleic acids of interest and at least one unwanted target sequence and wherein said bait molecule binds specifically to said unwanted target sequence, but not to said nucleic acids of interest, under such conditions as to allow for the formation of a bait:target complex; (b) digesting the unwanted target sequence in the bait:target complex thereby resulting in an increase in the relative percentage of said nucleic acids of interest within said mixed population of nucleic acids; fragmenting said nucleic acids of interest to produce fragments; and adding a label to the fragments.