Patent ID: 6905828
Filing Date: 2005-06-14
Classification: C12Q,G01N

Abstract:
1. A method of identifying human chromosomes comprising performing a multicolor FISH analysis using a set of nucleic acid probes for labeling one or more human chromosomes and hybridizing said one or more human chromosomes with said set of nucleic acid probes, said set of nucleic acid probes comprising a mixture of two interspersed repeated sequence-polymerase chain reaction (IRS-PCR) amplification products from said chromosome(s), wherein: the first amplification product is complementary to either a specific primer for Alu DNA sequences or a specific primer for long interspersed element (LINE) sequences; the second amplification product is complementary to a specific primer for Alu DNA sequences and a specific primer for LINE DNA sequences; and each of said probes, being specific for one or more human chromosomes, are directly or indirectly labeled with: a) a fluorophore having an absorption bandwidth wavelength interval spanning 593 nm and an emission bandwidth wavelength interval spanning 613 nm; and b) a fluorophore having an absorption bandwidth wavelength interval spanning 554 nm and an emission bandwidth wavelength interval spanning 568 nm; and c) up to five (5) fluorophores selected from the group consisting of: identifying said one or more labeled human chromosomes by fluorescence microscopy, wherein the overlapping of the absorption and emission bandwidth wavelength intervals between the different fluorophores is minimal and there is no overlapping between the absorption and emission maximum of the different fluorophores; and wherein each of said fluorophores having a specific absorption and emission wavelength is combined with a pair of optical filters, one for absorption and one for emission in a fluorescent microscope.