Patent ID: 7820878
Filing Date: 2010-10-26
Classification: A01K,C12N

Abstract:
1. A method for producing a cloned bovine, wherein the expression of both copies of the rag-2 gene have been knocked out and wherein said bovine lacks viable B- and T-cells, which comprises the following steps: (i) producing a donor bovine fibroblast cell or nucleus therefrom wherein the expression of one or both copies of the rag-2 gene has been eliminated by targeted disruption; (ii) fusing or inserting said donor fibroblast cell or nucleus into an enucleated bovine oocyte, to produce an embryo; (iii) introducing said embryo into a female bovine; and (iv) obtaining a cloned fetus or bovine animal that expresses the genotype of the donor fibroblast cell, in which the expression of one or both copies of the rag-2 gene has been eliminated; and (v) optionally, mating said bovine animal with another bovine animal wherein one copy of the rag-2 gene has been knocked out and selecting progeny wherein both copies of the rag-2 gene have been knocked out resulting in a lack of viable V-and T-cells in said bovine animal.