Patent ID: 6689391
Filing Date: 2004-02-10
Classification: C09B,Y10S

Abstract:
A non-polar and non-proteinaceous fluorescent dye obtained from an alcoholic extract of ovarian cells from the non bioluminescent marine organism Holothuria scabra, said dye having the following characteristics:i. having no decolorization when exposed to a reducing agent, ii. being a naturally produced compound, iii. orange in color in crude extract form, iv. bright orange in color in semi-purified form, v. emitting a variety of colors of the visible light spectrum, vi. having a pigment that is insoluble in water and alcohol, vii. having a pigment that is soluble in ether, viii. having pH 7.0, ix. not having a chromophore that can be reduced, x. emitting six different colored fluorescence at three different wavelengths of the UV and visible ranges, xi. emitting fluorescence of blue color emission in the 450 nm-470 nm range on excitation under light in the 330 nm-385 nm range, xii. emitting fluorescence of yellowish green color emission in the 510 nm-570 nm range on excitation under light in the 450 nm-480 nm range, xiii. emitting fluorescence of orange color emission in the 610 nm-650 nm range on excitation under light in the 510 nm-550 nm excitation range, xiv. emitting yellowish gray emission under light in the spectrum visible to the human eye, xv. emitting detectable fluorescence at a dilution range of 1:900,000 times xvi. the fluorescence of the dye persists even up to one year when preserved at about 4Â° C., xvii. the fluorescence of the dye is highly stable against long expospres to direct light, xviii. the fluorescence of the dye does not change when the dye is frozen, xix. the dye is nontoxic, xx. the dye is permeable to living cell membranes, xxi. the dye is impermeable to dead eukaryotic cells and dead prokaryotic cells, and xxii. the dye is not degraded after staining cell components.