Patent ID: 9040296
Filing Date: 2015-05-26
Classification: A61K,A61P,C12N

Abstract:
1. A process of production of influenza virus which comprises replicating an influenza virus in cells of an avian cell line wherein said cell line has been obtained by a method comprising the following steps: A) culturing avian embryonic stem cells in a cell culture medium containing the factors allowing their growth, wherein said factors comprise: B) passaging by modifying the culture medium so as to obtain the withdrawal of said exogenous growth factors and cytokines, of the serum, and of the feeder layer; C) establishing adherent or non-adherent cell lines capable of proliferating in the absence of said exogenous growth factors, serum, and inactivated feeder layer from said cell culture medium; wherein said process comprises an additional step of feeding the cells wherein said feeding step consists in a periodic addition of concentrated solutions of glutamine and D-glucose to the culture medium; and wherein glutamine concentration in the culture medium is maintained between 1 mM to 3 mM and D-glucose concentration in the culture medium is maintained between 1 g/l to 10 g/l.