Patent ID: 7211399
Filing Date: 2007-05-01
Classification: A61P,C07K,G01N

Abstract:
1. A method for determining the binding of a compound to a leptin receptor comprising the steps of: i.) bringing said compound into contact with a first fusion protein consisting of a leptin receptor and an energy donor protein, wherein said leptin receptor consists of a short isoform comprising Box1 intracellular domain free of Box 3 intracellular domain or a soluble form of leptin receptor which contains the leptin-binding region of the molecule and said energy donor protein is a luciferase, GFP or aequorin, and a second fusion protein consisting of a leptin receptor and an energy acceptor protein, wherein said leptin receptor consists of a short isoform comprising Box1 intracellular domain free of Box 3 intracellular domain or a soluble form of leptin receptor which contains the leptin-binding region of the molecule and said energy acceptor protein is DsRed, GFP or a mutant of GFP, ii.) adding an enzyme substrate when said donor protein is a luciferase or aequorin, and iii.) measuring the energy transfer wherein the energy transfer measured in the presence of said compound is compared to that measured in the absence of said compound wherein an increase in energy transfer in the presence of said compound compared to that measured in the absence of said compound is indicative of binding of a compound to a leptin receptor.