Patent ID: 8999667
Filing Date: 2015-04-07
Classification: C12N

Abstract:
1. A DNA molecule comprising a multicistronic transcription unit, wherein the multicistronic transcription unit comprises, from 5′ to 3′: a first anti-repressor (STAR) element that is STAR7 (SEQ ID NO:7), an anti-repressor (STAR) element that is STAR67 (SEQ ID NO:66), a polynucleotide encoding a first selectable marker polypeptide, wherein the polynucleotide encoding the first selectable marker polypeptide comprises a translation initiation sequence separate from that of a polynucleotide encoding a polypeptide of interest, does not comprise an ATG codon in the coding strand between the start codon of the first selectable marker polypeptide and the start codon of the polypeptide of interest, and comprises a GTG start codon or a TTG start codon, the polynucleotide encoding the polypeptide of interest, wherein the polynucleotide encoding the polypeptide of interest has a translation initiation sequence separate from that of a polynucleotide encoding a second selectable marker polypeptide, an internal ribosome entry site (IRES), the polynucleotide encoding a second selectable marker polypeptide, wherein the polynucleotide encoding the second selectable marker polypeptide is under translational control of the IRES and comprises a GTG start codon, a TTG start codon, a CTG start codon, an ATT start codon or an ACG start codon, a transcription terminator, and a second anti-repressor (STAR) element that is STAR7 (SEQ ID NO:7).