Patent ID: 8409794
Filing Date: 2013-04-02
Classification: C07F,G01N

Abstract:
1. A method for determining prostate cancer, or the risk of prostate cancer in a patient, the method comprising the steps of: a) analyzing at least one blood sample from said patient by high resolution mass spectrometry to obtain accurate mass intensity data; b) comparing the accurate mass intensity data to corresponding data obtained from one or more than one reference blood sample to identify an increase or decrease in accurate mass intensity; and c) using said increase or decrease in accurate mass intensity to determine prostate cancer, or the risk of prostate cancer in said patient, wherein the accurate mass intensity is measured, in Daltons, at one or more of the following hydrogen and electron adjusted accurate masses or neutral accurate masses ±5 ppm, wherein the hydrogen and electron adjusted accurate masses or neutral accurate masses at which intensity decreases are: 174.1408, 188.1566, 194.0804, 232.9133, 242.2251, 252.2096, 258.2482, 268.2412, 272.2357, 276.2096, 278.2256, 279.2287, 280.2414, 281.2448, 283.2602, 292.204, 296.2358, 298.2519, 299.2558, 300.2098, 300.2676, 302.2256, 304.2394, 304.241, 305.243, 305.2439, 306.257, 308.2715, 310.2154, 310.2884, 312.2313, 312.304, 314.2464, 320.2358, 326.2262, 327.0326, 329.2426, 329.2445, 330.2568, 340.2977, 342.2198, 368.3437, 369.3474, 371.3538, 392.294, 411.3186, 430.3083, 430.3818, 431.3861, 432.3686, 452.2536, 481.3171, 481.3172, 482.3216, 484.3792, 492.4184, 494.4344, 495.3328, 495.4376, 496.336, 501.2848, 505.3227, 506.3213, 507.3317, 509.3493, 517.3148, 518.3182, 518.4345, 519.332, 519.3328, 520.4502, 521.348, 521.4526, 522.464, 523.364, 523.4678, 524.4725, 529.3167, 531.3123, 534.4645, 538.501, 541.3148, 541.3422, 541.3433, 542.3453, 542.3461, 545.346, 548.4817, 549.4848, 552.4048, 555.3101, 565.3393, 565.3394, 566.3433, 566.3434, 567.3546, 567.3548, 568.3573, 568.3574, 569.3687, 569.3691, 570.3726, 570.4653, 570.4915, 579.5322, 580.5345, 587.3228, 589.3401, 589.3404, 590.343, 590.4597, 596.4794, 599.4932, 601.5077, 604.5441, 605.5469, 609.3242, 612.5004, 615.4797, 622.4973, 623.4918, 623.5003, 624.5134, 625.5078, 625.5163, 626.5109, 626.5285, 627.5204, 627.5306, 628.5236, 628.5426, 629.5453, 630.5582, 632.5752, 635.5246, 641.4915, 646.5709, 647.574, 647.6063, 648.5865, 649.5056, 649.5898, 655.5509, 660.5005, 660.6082, 663.4864, 670.5688, 670.5711, 671.5723, 672.5865, 673.5893, 673.6185, 673.6224, 675.6359, 675.6375, 676.6393, 680.5625, 684.5487, 685.5543, 686.5126, 688.5294, 690.4849, 690.547, 692.5571, 693.611, 695.647, 696.5856, 696.651, 699.5205, 702.5675, 705.6083, 707.6256, 708.6308, 710.4923, 716.4982, 721.6388, 722.6423, 723.5194, 723.5198, 724.5247, 724.5496, 725.5375, 726.5456, 727.5565, 728.562, 729.5724, 731.4913, 732.4938, 733.6425, 735.6555, 736.6584, 737.5354, 738.5449, 741.5307, 742.5354, 743.5469, 744.4942, 745.4972, 746.556, 747.5201, 747.5234, 748.5279, 748.5722, 749.5346, 749.5354, 749.5364, 749.5402, 749.5763, 750.5403, 750.5434, 751.551, 751.5529, 751.5548, 752.5565, 752.5578, 753.5674, 755.4866, 756.4905, 757.5017, 757.5618, 758.5089, 758.5654, 759.516, 759.578, 760.5223, 760.5816, 761.5269, 765.5665, 766.5701, 767.5821, 768.4944, 768.5507, 769.4957, 770.5109, 771.5809, 772.5269, 772.5856, 773.5337, 774.5404, 775.553, 775.5533, 776.5563, 776.6057, 777.5679, 779.5438, 779.5831, 780.5474, 781.5612, 782.5087, 782.5649, 783.5141, 783.578, 784.5235, 784.5813, 785.5295, 785.5936, 786.5404, 786.5967, 787.5447, 793.5387, 794.5126, 794.5424, 795.5555, 796.5292, 798.6776, 803.5436, 803.5685, 804.547, 804.5717, 804.7208, 804.7219, 805.5606, 805.5834, 805.7267, 806.5643, 806.5863, 807.5761, 808.5795, 809.5937, 810.5401, 810.597, 811.5733, 812.5767, 813.5888, 814.592, 816.5591, 816.7297, 817.5376, 819.5553, 821.5718, 822.5751, 824.689, 825.5545, 825.5548, 826.5579, 826.7053, 827.5439, 827.5698, 827.5699, 827.7084, 828.5734, 828.5741, 828.7206, 829.5597, 829.5857, 829.7239, 829.7244, 830.5887, 830.6537, 830.7359, 831.575, 831.5999, 831.6002, 831.7409, 832.5765, 832.6028, 832.6039, 835.6996, 836.7063, 837.5884, 837.7182, 838.7227, 839.7321, 847.5955, 850.703, 851.5689, 851.7111, 852.5725, 852.7198, 852.7242, 853.5854, 853.7252, 854.5887, 855.6013, 856.6046, 856.6697, 857.617, 857.6733, 858.6847, 859.6877, 861.5265, 861.7174, 861.7808, 862.7228, 863.6874, 863.7339, 864.738, 865.7482, 866.7527, 867.7576, 871.5528, 872.5556, 873.5684, 876.7223, 877.7271, 878.7381, 879.598, 879.742, 880.7528, 880.7555, 881.7568, 881.7609, 882.7673, 882.7717, 883.7715, 884.7817, 884.7873, 885.7867, 885.7919, 886.5582, 886.8027, 887.5625, 887.8022, 893.774, 894.7273, 894.7813, 895.5578, 895.559, 895.7335, 895.7873, 896.745, 897.573, 897.75, 898.7605, 899.5871, 899.7663, 900.5897, 902.737, 903.7407, 904.7535, 905.7573, 906.769, 907.7735, 908.708, 908.7842, 908.7843, 909.7153, 909.7892, 910.7248, 910.7979, 911.7326, 912.7412, 913.7502, 914.7577, 915.7673, 916.774, 917.7836, 918.7901, 919.7981, 920.747, 920.8054, 921.753, 921.8145, 922.7656, 922.8229, 923.5884, 923.7675, 924.7826, 926.7371, 928.7519, 930.7673, 931.7691, 931.793, 932.7819, 934.7235, 935.7299, 936.7387, 937.7457, 938.7553, 939.7616, 940.7709, 941.7779, 942.7876, 943.7931, 944.8033, 945.8085, 946.8187, 950.7385, 952.7568, 952.7759, 954.7905, 962.7616, 964.7764, 965.7839, 966.7933, 967.7981, 968.8072, 1016.929, 1017.934, 1018.943, 1019.95, 1040.934, 1225.093, 1226.098, 1227.107, 1228.113, 1229.118, 1251.115, and 1253.129, and a decrease in the accurate mass intensity at one or more of said accurate masses in the blood sample from the patient relative to the reference indicates that the patient has prostate cancer or is at risk of prostate cancer; and wherein the hydrogen and electron adjusted accurate masses or neutral accurate masses at which intensity increases are: 202.0454, 205.8867, 216.0401, 218.0372, 226.0687, 228.1476, 243.0719, 244.056, 247.9578, 273.874, 283.9028, 317.9626, 326.2476, 328.2628, 331.8326, 339.9964, 341.8614, 351.8906, 354.1668, 382.2903, 472.3925, 473.3957, 552.3825, 582.2469, 583.2504, 736.2234, 776.6069, 783.6349, 997.3968, 1176.777 and 1373.744, and an increase in the accurate mass intensity at one or more of said accurate masses in the blood sample from the patient relative to the reference indicates that the patient has prostate cancer or is at risk of prostate cancer.