Patent ID: 8268247
Filing Date: 2012-09-18
Classification: G01N

Abstract:
1. An electrophoresis extraction device, comprising: a base having a transport passage, a transport passage inlet port, a transport passage outlet port, the transport passage communicating with the transport passage inlet port and the transport passage outlet port, the base having a first extraction device comprising a first separation passage first port, a first separation passage second port, and a first separation passage communicating with the first separation passage first port and the first separation passage second port, the base having a second extraction device comprising a second separation passage first port, a second separation passage second port, a second separation passage communicating with the second separation passage first port and the second separation passage second port and an exit passage into which the second port of the first and second separation passages merge to create a single outlet passage; a first reservoir for a separation buffer supply, eluting buffer supply or cleaning solution supply communicating with the first separation passage first port, a second reservoir for a separation buffer supply, eluting buffer supply or cleaning solution supply communicating with the second separation passage first port, the first and second separation passages within the corresponding extraction devices each including an elongated passage portion; between the transport passage inlet port and the transport passage outlet port, the transport passage intersecting as a staggered configuration intersection with the elongated passage portion of each of the first and second separation passages within each corresponding extraction device; the elongated passage portion of the first separation passage within the first extraction device defining an area for holding a first means for capturing and concentrating a first analyte of interest in a sample, the elongated passage portion of the second separation passage within the second extraction device defining an area for holding a second means for capturing and concentrating a second analyte of interest in the sample, the sample passing from the transport passage inlet port through the staggered configuration intersection with the elongated passage portion of each of the first and second separation passages within each corresponding extraction device to the transport passage outlet port and the concentrated first and second analyte of interest to be released from the elongated passage portion of each of the first and second separation passages by alternating fluid communication of the separation buffer supply and then the eluting buffer supply from the respective first reservoir and second reservoir and the first and second analyte of interest being delivered out the respective second port of the first and second separation passages to the exit passage separately, independently and sequentially by pressure, electrophoresis migration or a combination of electro-osmotic flow and pressure and to an analyte detection system associated with the exit passage; a first auxiliary buffer passage communicating with the first separation passage and a second auxiliary buffer passage communicating with the second separation passage, the first and second auxiliary buffer passages positioned downstream of the respective means for capturing and concentrating a first analyte of interest and a second analyte of interest and before the second ports of the first and second separation passages merge into the single outlet passage; and a valve system operatively associated with the transport passage and the first and second elongated passage portions of the corresponding extraction devices for independently controlling the flow of the sample in the transport passage and separately and independently forming a microenvironment in the first and second separation passages to mix reagents to optimize the binding and capture of the first and second analytes of interest by the respective means for capturing and concentrating the first and second analytes of interest, wherein the elongated passage portion of the first and second separation passages of the base of each of the corresponding extraction devices defining an extraction device or analyte concentrator-microreactor device.