Patent ID: 8623632
Filing Date: 2014-01-07
Classification: C12N,C12P

Abstract:
1. A genetically engineered Pseudomonas putida having at least one modification in at least one gene encoding a protein involved in the metabolism of polyhydroxyalkanoate(s) (PHA), wherein the protein is a thioesterase acting on (R)-3-OH-acyl-CoA as a substrate which competes with enzymes of the PHA synthesis pathway for intermediates of the PHA synthesis pathway, wherein the at least one modification causes deposition and/or overproduction of medium or long chain PHA relative to PHA production of the corresponding wildtype Pseudomonas putida , wherein the genetically engineered Pseudomonas putida further comprises a modification in the 1-acyl-sn-glycerol-3-phosphate acyltransferase gene which inactivates the gene.