Patent ID: 7445893
Filing Date: 2008-11-04
Classification: C12Q

Abstract:
1. A method of analyzing a nucleic acid amplification comprising: providing a nucleic acid amplification reaction mixture comprising a plurality of different amplification templates; subjecting said reaction mixture to an amplification regimen; dispensing or withdrawing an aliquot from said reaction mixture at plural stages during said amplification regimen; separating and detecting nucleic acids in said aliquot, wherein said separating comprises capillary electrophoresis; determining the quantity of a plurality of separated nucleic acid species in said aliquot, wherein said plurality of different amplification templates comprises at least five different amplification templates and for each said separated nucleic acid species from each said stage, correlating the quantity of said species with the stage at which said aliquot comprising said species was dispensed, wherein said correlating generates an amplification profile of said nucleic acid amplification, and wherein said amplification profile provides quantitative information regarding the abundance of said plurality of different amplification templates in said amplification reaction mixture at the start of said amplification regimen.