Patent ID: 8124379
Filing Date: 2012-02-28
Classification: C07K,C12N

Abstract:
1. A method for producing a secreted polypeptide, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of a polypeptide, wherein the host cell comprises a nucleic acid construct comprising a first nucleotide sequence encoding a signal peptide operably linked to a second nucleotide sequence encoding the polypeptide, wherein the first nucleotide sequence is foreign to the second nucleotide sequence, the 3′ end of the first nucleotide sequence is immediately upstream of the second nucleotide sequence, and the first nucleotide sequence is selected from the group consisting of: (b) isolating the secreted polypeptide from the cultivation medium; wherein the signal peptide retains the ability to direct the polypeptide into or across a cell membrane.