Patent ID: 9131668
Filing Date: 2015-09-15
Classification: A01K,A61K,C12N

Abstract:
1. A mouse tumor model system comprising adhesion deprived cells capable of metastasizing at one or more sites prepared by a method comprising: (a) trypsinizing normal adherent F111 rat fibroblast cells; (b) suspending the trypsinized F111 rat fibroblast cells on a surface coated with agarose in a medium containing Fetal Calf Serum (FCS) to obtain adhesion-deprived F111 rat fibroblast cells; and (c) administering subcutaneously the F111 adhesion-deprived rat fibroblast cells to an immunocompromised mouse to form tumors or to metastasize, or both to form tumors and to metastasize in the immunocompromised mouse; wherein the normal adherent F111 rat fibroblast cells are non-transfected cells.