Patent ID: 6699662
Filing Date: 2004-03-02
Classification: C12Q

Abstract:
A method of identifying inhibitors of the binding of SarA involved in the expression of staphylococcal virulence genes to at least one SarA binding site comprising:a) contacting a candidate inhibitor with at least one SarA binding site of the agr locus in solution to allow the binding reaction to equilibrate for a sufficient period of time, wherein said SarA binding site of the agr locus as depicted in FIG. 3 is at least one nucleotide sequence selected from the group consisting of at least the nucleotide sequences in the A1 (SEQ ID NO:12) and A2 (SEQ ID NO:14) boxes, at least the nucleotide sequences in the B1 (SEQ ID NO:16) and B2 (SEQ ID NO:17) boxes and at least the nucleotide sequences in the C1 (SEQ ID NO:15) and C2 (SEQ ID NO:13) boxes; b) assessing the binding of said candidate inhibitor to said SarA binding site of the agr locus; and c) selecting said candidate inhibitor that binds to the agr locus.