Patent ID: 6274353
Filing Date: 2001-08-14
Classification: C07K,C12N,C12P,C12Q

Abstract:
A method for selectively amplifying a target polynucleotide molecule, comprising:(A) contacting a specimen suspected of containing said target polynucleotide molecule with a reaction mixture comprising (1) a primer complementary to said target polynucleotide molecule, wherein the 3'-hydroxyl terminus of the primer is protected with a blocking group that prevents chain-elongation; (2) a thermostable blocking group removing enzyme (TBGRE); (3) a thermostable polynucleotide polymerase; (4) at least one nucleoside-5'-triphosphate; and(B) elevating the temperature of the resulting mixture to higher than about 38.degree. C., thereby generating a primer extension product.