Patent ID: 6236944
Filing Date: 2001-05-22
Classification: G01N,G16B

Abstract:
A method of analyzing DNA sequencing electropherograms comprising the steps of:obtaining a signal produced by a sequence of electrophoretically separated DNA fragments;utilizing an expert system including a knowledge base and an inference engine to determine a plurality of base-calls from said signal,wherein said inference engine executes at least one rule for measuring properties of a plurality of peaks to estimate a number of base-calls, andwherein said rule for measuring properties of a plurality of peaks to estimate a number of base calls comprises the steps of:determining a height for a singleton peak;determining a full width at half height for said singleton peak;determining base spacing; andcalculating a number of base-calls by subtracting said full width at half height for said singleton peak from a measured width at said half height for a selected one of said plurality of peaks to obtain a first value, dividing said first value by said base spacing to obtain a second value, and adding 1 to said second value.