Patent ID: 7166208
Filing Date: 2007-01-23
Classification: C12Q,G01N

Abstract:
1. An electrochemical detection device for detecting an antigen in a liquid sample, said detection device comprising: an electrode containing an apoenzyme or otherwise inactive form of an electrochemically active enzyme that, in the active form, would produce an electrochemical change in said electrode in response to a substrate to the electrically active enzyme; an apoenzyme cofactor, prosthetic group, or other activation moiety that converts the inactive form of said electrochemically active enzyme to an active form, said cofactor or activation moiety being present in the form of a complex that changes its structure due to interactions with said antigen; said complex additionally containing an antibody capable of binding to said antigen; said complex being incapable of activating the apoenzyme or otherwise inactive form of the electrochemically active enzyme in the absence of said antigen; wherein said antigen induces changes in said complex, enabling said cofactor, prosthetic group or said activation moiety to activate said apoenzyme or said inactive form of an electrochemically active enzyme: resulting in a detectable electrochemical change in said electrode.