Patent ID: 6410253
Filing Date: 2002-06-25
Classification: C12N,C12Q,Y10S

Abstract:
A method for quantitative determination of cholesterol contained in a sample which comprises;exposing the sample to a cholesterol oxidase to oxidize the cholesterol, and measuring the amount of oxidized cholesterol or hydrogen peroxide thereby determining the amount of the cholesterol, wherein the cholesterol oxidase is coded by DNA which is hybridizable to DNA as defined in SEQ ID: NO:1, under stringent conditions, wherein said stringent conditions comprise hybridization at 45Â° C. in a hybridization buffer containing 53.0 g/l NaCl, 26.5 g/l sodium succinate, 10 mM EDTA, 5-fold Denhart solution, 0.5% SDS, 0.1 mg/ml thermally denatured bovine thymus-derived DNA for 16 hours and washing at 65Â° C. with 3-fold SSC.