Patent ID: 7442507
Filing Date: 2008-10-28
Classification: C12Q

Abstract:
1. A method for diagnosing metastatic melanoma in a subject, said method comprising: (a) isolating a blood sample from said subject, wherein the blood sample comprises DNA sequences; (b) performing an amplification reaction of said DNA sequences of said blood sample, wherein said amplification reaction comprises a first primer capable of annealing specifically to a BRAF mutant sequence at a first position in a BRAF DNA sequence wherein said first primer is SEQ ID NO: 6 and a second primer capable of annealing specifically at a second position in a BRAF DNA sequence, wherein said first and second primers anneal to different strands of double stranded BRAF DNA sequence, wherein the amplification reaction is capable of producing a BRAF mutant specific amplification product when the DNA sequences of the sample comprise a BRAF DNA sequence comprising a mutant sequence at said first position of said BRAF DNA sequence; and (c) visualizing amplification products produced by said amplification reaction, wherein detection of a BRAF mutant specific amplification product is a positive indicator of metastatic melanoma in said subject.