Patent ID: 6103955
Filing Date: 2000-08-15
Classification: C12N

Abstract:
A procedure for the genetic transformation of citrus adult plants consisting of:co-culturing explants of adult tissue from citrus plant species, from the first flushes of the grafts of buds of citrus adult plants onto stocks, with a non-oncogenic strain of Agrobacterium tumefaciens, optionally modified to further contain genes which encode the characteristics of interest to be inserted into the citrus plant, in feeder plates, and subsequently, in a culture medium which favors the induction of transgenic shoots and permits the selection thereof;in vitro micrografting said transgenic shoots, their buds or apices, onto stocks cultivated in vitro; andgrafting the resulting in vitro micrografted plants, their buds and apices, onto other stocks which give vigor and allowing the successful grafts to grow to generate complete adult plants, or directly transplanting the in vitro micrografted plants into the soil to generate complete adult plants.