Patent ID: 8195402
Filing Date: 2012-06-05
Classification: G01N

Abstract:
1. A method for determining or typifying in vitro well or fully differentiated mammal cells comprising the steps of: a) irradiating the well or fully differentiated mammal cells with infrared light and recording the absorption spectrum obtained; b) carrying out a Fourier transformation of the absorption spectrum from (a) to obtain an FT-IR spectrum; c) calculating the first derivative and, optionally, higher derivatives of the FT-IR spectrum of the well or fully differentiated mammal cells using a computer program to calculate the first derivative and optionally higher derivatives of the FT-IR spectrum when the absorption spectrum from (b) is substantially similar to a underivatized reference FT-IR spectrum; d) comparing the derivative(s) from step (c) in a preselected wave number range with the same derivative(s) of at least one reference FT-IR spectrum; and e) displaying the result of the comparison of step (d); wherein the result indicates whether the cells are suitable for transplantation; wherein the substantial similarity between spectra in step (c) is present when the well or fully differentiated cells and reference cells exhibit a heterogeneity of less than about 1.7, as determined by cluster analysis.