Patent ID: 7670825
Filing Date: 2010-03-02
Classification: C12N,C12P

Abstract:
1. A genetically modified host cell that produces an isoprenoid or isoprenoid precursor, wherein the genetically modified host cell is a prokaryotic host cell that does not normally produce isopentenyl pyrophosphate via a mevalonate pathway, wherein the genetically modified host cell is genetically modified with one or more heterologous nucleic acids comprising nucleotide sequences encoding: i) an enzyme that condenses acetoacetyl-CoA with acetyl-CoA to form HMG-CoA (HMGS); ii) an enzyme that converts HMG-CoA to mevalonate (HMGR); iii) an enzyme that converts mevalonate to mevalonate 5-phosphate; iv) an enzyme that converts mevalonate 5-phosphate to mevalonate 5-pyrophosphate; and v) an enzyme that converts mevalonate 5-pyrophosphate to isopentenyl pyrophosphate, wherein the ratio of HMGR activity to HMGS activity in the cell is at least 1, and wherein the copy number of HMGR- and HMGS-encoding nucleotide sequences in the cell is higher than in a cell in which HMGR and HMGS are encoded on a low copy number pBAD33 plasmid and operably linked to an arabinose inducible P