Patent ID: 8877452
Filing Date: 2014-11-04
Classification: C12Q,G01N

Abstract:
1. A method for screening inhibitors against nitrite reductase useful as anti-tubercular drug comprising: i. infecting Thp1 macrophage culture in wells of a plate with ii. washing the infected macrophage culture with 1×PBS solution followed by adding fresh medium containing 50 mM of sodium nitrate; iii. adding 2.5 μl of a test inhibitor(s) against nitrite reductase in DMSO to the infected macrophage culture in a well of the plate on 0 d; iv. adding 2.5 μl of one or more standard inhibitors of narG, nirB and glnA1 at their respective IC v. estimating the level of ammonia/nitrite in the culture medium for determining the viable cell count; and vi. determining reduction in the viable cell count by the test inhibitor(s) as compared to the standard inhibitor(s) to obtain an anti-tubercular drug target.