Patent ID: 9101943
Filing Date: 2015-08-11
Classification: B01L,B04B,C12M,G01N

Abstract:
1. A method of particle separation using a centrifuge and a centrifugal and detection device disposed thereon, comprising: putting an object to be separated and detected into a base of the centrifugal and detection device, wherein the centrifugal and detection device comprises at least two concentric walls disposed on the base to delimit a plurality of annular channels, an outer wall disposed on the base and surrounding the concentric walls at an outermost periphery, and a lid upwardly covering the concentric walls and the outer wall, the lid having an inlet for loading the object to be detected, wherein the concentric walls comprises a plurality of apertures communicating with the annular channels: rotating the centrifuge to allow the object to move through the annular channels and the apertures according to its size; intermittently manually touching a rotary plate of the centrifuge to instantly stop the centrifuge, thereby generating a lateral flow for decreasing clogging during the rotating step; and resulting in a target substance of the object being retained in an inner annular channel after centrifugation, wherein the object is directly loaded to a central annular channel through the inlet; wherein the at least two concentric walls comprises a first concentric wall and a second concentric wall, wherein an inner radius of the second concentric wall is greater than an inner radius of the first concentric wall, wherein all of the apertures through the first concentric wall are identical in size and all of the apertures through the second concentric wall are identical in size, and the apertures through the first concentric wall are larger than the apertures through the second concentric wall.