Patent ID: 8399195
Filing Date: 2013-03-19
Classification: C12Q

Abstract:
1. A method of quantifying relative amounts of maternal and fetal alleles of interest in a sample comprising: amplifying nucleotide sequences comprising a tandem single nucleotide polymorphism (SNP) from a sample comprising fetal and maternal nucleic acids, wherein a single pair of primers is used to amplify each of the nucleotide sequences; detecting the amplified nucleotide sequences; identifying amplified nucleotide sequences that comprise three alleles of the tandem SNP, wherein two of the three amplified nucleotide sequences comprise maternal alleles of the tandem SNP in the maternal nucleic acid and one of the amplified nucleotide sequences comprises a fetal allele of the tandem SNP in the fetal nucleic acid that is absent in the maternal nucleic acid; quantifying amounts of maternal alleles of the tandem SNP and quantifying an amount of fetal allele of the tandem SNP; and comparing the amount of maternal alleles of the tandem SNP to the amount of a fetal allele of the tandem SNP to determine the amount of fetal nucleic acid relative to maternal nucleic acid in the sample.