Patent ID: 8415133
Filing Date: 2013-04-09
Classification: A61K,A61P,C07K,C12N

Abstract:
1. A process for producing a composition of a wild type, an attenuated, and/or a recombinant poxvirus with no targeted infection specificity comprising the steps of: a) preparing a cell culture of packaging cells, b) infecting said cell culture to obtain infected cells, c) culturing said infected cells for an appropriate period of time to produce poxviral particles, d) recovering said poxviral particles from the culture supernatant and from packaging cells disrupted by high speed homogenizer, to obtain a poxviral particle mixture, e) clarifying said poxviral particle mixture, thereby removing cellular debris, wherein said clarifying comprises depth filtration, to obtain a clarified poxviral particle mixture, f) concentrating said clarified poxviral particle mixture, wherein said concentrating comprises microfiltration, to obtain a concentrated poxviral particle mixture, and g) diafiltrating said concentrated poxviral mixture, wherein said process is free from animal products and does not use nuclease, wherein said poxvirus composition comprises intracellular mature virus (“IMV”) and extracellular enveloped virus (“EEV”) with more than 1% EEV, and wherein said packaging cells are chicken embryo fibroblast (“CEF”) prepared by enzymatic digestion.