Patent ID: 6235501
Filing Date: 2001-05-22
Classification: C12N

Abstract:
A method of isolating high molecular weight nucleic acid from a biological material which comprises mechanically releasing said high molecular weight nucleic acid from said material by the application of rapidly oscillating reciprocal mechanical energy to said material in the presence of a liquid medium in a closed container to produce a released high molecular weight nucleic acid solution, wherein said released high molecular weight nucleic acid has an average molecular weight greater than 10 kilobases, said liquid medium contains one or more particles and detergent in a amount of from about 0.1% to 10% weight per weight (w/w), and said application of said energy is conducted by subjecting said container and thereby said material to oscillations at an oscillatory rate of between about 25 hertz (Hz) to about 166 Hz for a period of time of between about 3 seconds to about 5 minutes.