Patent ID: 6589749
Filing Date: 2003-07-08
Classification: A61B,B01L,G01N,Y10S,Y10T

Abstract:
A method for processing fluid containing cells, comprising:using a pump, drawing fluid containing a ligand and cells from a specimen cup; passing the fluid through a first housing having a chamber containing chromatographic bed material, and into the pump, under conditions which allow the ligand to bind to the chromatographic bed material; reversing the direction of the fluid flow and passing the mixture of the chromatographic material and the fluid sample through the first housing and into the specimen cup; separating the first housing containing the chromatographic bed material from the pump; connecting a second housing to the pump, said second housing having a filter membrane disposed therein for capturing cells in a monolayer; using the pump, drawing fluid containing cells from the specimen cup through the second housing under conditions which allow cells in the fluid to be collected in a monolayer on the membrane; and separating the second housing containing the collected cells from the pump.