Patent ID: 6710200
Filing Date: 2004-03-23
Classification: C07C,C07D,C07K

Abstract:
A method of detecting in a sample the presence of enzyme activity that converts an ether or ester group in a substrate to an alcohol group, said method comprising:(a) contacting said sample with a reporter having the formula in which:R1 is an aldehyde or ketone group that is optically detectable by fluorescence emission, UV light absorptivity, or colorimety; R2 is a member selected from the group consisting of H, C1-C2 alkyl, benzyl, and substituted benzyl; and R3 is a group that provides said reporter with a molecular structure that upon exposure to said enzyme activity causes R3 to be replaced by H, R3 further being a member selected from the group consisting of alkyl, substituted alkyl, aralkyl, substituted aralkyl, phosphate, sulfate, carbohydrate residues, substituted carbohydrate residues, and â€ƒin which R4 is a member selected from the group consisting of alkyl, substituted alkyl, aralkyl, substituted aralkyl, oxiranylalkyl, substituted oxiranylalkyl, oxiranylalkoxy, substituted oxiranylalkoxy, alkylamino, substituted alkylamino, peptidylamino, cycloalkylamino, substituted cycloalkylamino, oxiranylamino, substituted oxiranylamino, alkoxy, substituted alkoxy, and a residue of the acid moiety of a pyrethroid, under conditions by which said enzyme activity if present would promote the conversion of said reporter to a cyanohydrin of the formula and the rearrangement of said cyanohydrin to a carbonyl compound of the formula and(b) detecting said carbonyl compound by optical detection selectively relative to said cyanohydrin and said reporter.