Patent ID: 6136561
Filing Date: 2000-10-24
Classification: A61K,C07K,C12N,G01N,Y02A

Abstract:
An expression system for the recombinant production and secretion of a portion of an envelope (E) protein of a Flavivirus selected from the group consisting of dengue virus, Japanese encephalitis virus (JEV), tick-borne encephalitis virus (TBE) and yellow fever virus (YF), which expression system comprises Drosophila cells modified to contain a DNA molecule which comprises(a) a first nucleotide sequence encoding said portion of said E protein of the Flavivirus strain against which protection is sought, which portion is the N-terminal 80% of the protein from residue 1 to residue 395, and(b) a second nucleotide sequence which encodes a secretory leader sequence or a secretory signal sequence operably linked to said first nucleotide sequence and positioned so as to produce a fusion protein when said first and said second nucleotide sequences are expressed in a eucaryotic cell,said encoding sequences operably linked to control sequences capable of effecting expression of said encoding nucleotide sequences in eucaryotic cells.