Patent ID: 7625584
Filing Date: 2009-12-01
Classification: A61K,A61P,C07K,C12N,C12P,C12Y

Abstract:
1. A method for complexing a protein having biological activity with liposomes, said method comprising: (a) providing a protein; (b) altering the conformational state of the protein to expose hydrophobic domains therein by contacting said protein with a mixture of water and ethanol at a concentration of 10% (v/v) at a temperature of 37° C. or higher; (c) separately preparing liposomes comprising phospholipids selected from the group consisting of phosphatidylserine (PS), phosphatidylchloline (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG) and combinations thereof; (d) contacting the liposomes from (c) with the protein having an altered conformational state from (b); and (e) at least partially reversing said altered conformational state of the protein complexed with the liposomes from (d) by removal of the ethanol from the mixture; wherein the protein has biological activity.