Patent ID: 8921040
Filing Date: 2014-12-30
Classification: B01J,B01L,G01N

Abstract:
1. A method of pyrosequencing a polynucleotide molecule, said method comprising the steps of: providing a rotatable platform adapted to bind a polynucleotide molecule in one or more discrete uncovered areas on an upper surface of said rotatable platform; binding said polynucleotide molecule to one or more of said discrete uncovered areas on said upper surface of said rotatable platform; annealing an oligonucleotide primer to a single strand of said polynucleotide molecule; and dispensing onto each said discrete uncovered area from a point external of said platform a series of pyrosequencing reagents, wherein after one or more or all of said dispensing steps said platform is rotated sufficiently such that any residual or unreacted said reagent is substantially centrifugally removed from each discrete area and off said platform; assaying for the presence of a pyrophosphate group; and repeating said dispensing and assaying steps, thereby sequencing said polynucleotide molecule.