Patent ID: 7358049
Filing Date: 2008-04-15
Classification: C12Q

Abstract:
1. A method of identifying a gene or genes involved in transcription-dependent memory comprising the steps of: (a) training non-human animals under conditions sufficient to induce transcription-dependent memory formation in said non-human animals; (b) extracting RNA from brain tissue of said non-human animals trained in step (a); (c) synthesizing DNA probes using the RNA extracted in step (b); (d) exposing the DNA probes synthesized in step (c) to microarray chips containing DNA sequences from genes of the genome of said non-human animals under conditions appropriate for hybridization of the DNA probes to complementary DNA sequences on the microarray chips, wherein a signal is produced upon hybridization of said probes to complementary DNA sequences; (e) detecting the signal produced in step (d); and (f) performing a statistical comparison between the signal detected in step (e) and the signal detected in a control, wherein said control is obtained according to a method comprising the steps of: (i) training non-human control animals under appropriate conditions, wherein said conditions are insufficient to induce transcription-dependent memory formation in said non-human control animals; (ii) extracting RNA from brain tissue of said non-human control animals trained in step (f)(i); (iii) synthesizing DNA probes using the RNA extracted in step (f)(ii); and (iv) exposing the DNA probes synthesized in step (f)(iii) to microarray chips containing DNA sequences from genes of the genome of non-human control animals under conditions appropriate for hybridization of the DNA probes to complementary DNA sequences on the microarray chips, wherein a signal is produced upon hybridization of said probes to complementary DNA sequences.