Patent ID: 6287766
Filing Date: 2001-09-11
Classification: C12Q

Abstract:
A homogeneous method for determining the identity of a base on a DNA strand, which comprises in order the steps of:(a) immobilizing an oligonucleotide which is capable of being annealed to the DNA strand immediately adjacent to the base whose identity is to be determined on a microsphere;(b) annealing the oligonucleotide to the DNA strand;(c) incubating the microspheres to which the DNA strand has annealed to the immobilized oligonucleotide with four different dideoxynucleotides, one of the dideoxynucleotides having a reporter molecule in the presence of an enzyme, thereby extending the immobilized oligonucleotide by one base unit; and(d) analyzing the microsphere using flow cytometry whereby the identity of the base is determined.