Patent ID: 6159748
Filing Date: 2000-12-12
Classification: C12Q,G01N,Y10S

Abstract:
A fluorescent immuno-bead non-agglutination, sandwich assay kit for use in conjunction with flow cytometry for the simultaneous detection in sera of the antinuclear antibodies to RNP (ribonucleoprotein) selected from the group consisting of mixed connective disease, systemic lupus erythematosis (SLE), Sjogren's syndrome, scleroderma and polymyositis, Sm (Smith antigen) in SLE; SS-A in Sjogren's syndrome and SLE, SS-B in Sjogren's syndrome and SLE and Scl-70 in scleroderma, using a flow cytometer to analyze at least the size and the fluorescence of a plurality of single microsphere complexes each including a bead carrier, a bound antigen or analyte, a primary antibody against the bound antigen, a secondary anti-species antibody attached to the primary antibody, and an indicator attached to the anti-species antibody, comprising:4 .mu.m particle sized latex beads,5 .mu.m particle sized latex beads,6 .mu.m particle sized latex beads,7 .mu.m particle sized latex beads,10 .mu.m particle sized latex beads,Sm/RNP Complex antigen,Sm antigen,SS-A (Ro) antigen,SS-B (La) antigen,Scl-70 antigen,lyophilized Anti-RNP,lyophilized Anti-Sm,lyophilized Anti-SS-A (Ro)lyophilized Anti-Sm,lyophilized Anti-SSB (La)lyophilized Anti-Scl-70.Goat anti-human IgG F(ab').sup.2 -[FITC] fluorescein isothiocyanlate,Sodium Carbonate,Sodium Bicarbonate, andBovine Serum Albumin.