Patent ID: 7592160
Filing Date: 2009-09-22
Classification: C12P

Abstract:
1. A process for producing isomaltitol, comprising the steps of: (a) allowing an α-isomaltosylglucosaccharide-forming enzyme, which forms an α-isomaltosylglucosaccharide with a glucose polymerization degree of at least three and having both the α-1,6 glucosidic linkage as the linkage at the non-reducing end and the α-1,4 glucosidic linkage other than the above linkage, via the α-glucosyl-transfer from a material saccharide having a glucose polymerization degree of at least two and having the α-1,4 glucosidic linkage as the linkage at the non-reducing end, without substantially increasing the reducing power of the material saccharide, to act on a saccharide with a glucose polymerization degree of at least two and having the α-1,4 glucosidic linkage as the linkage of non-reducing end to form said α-isomaltosylglucosaccharide wherein said α-isomaltosylglucosaccharide-forming enzyme has the following physicochemical properties: (1) Molecular weight (2) Isoelectric point (3) Optimum temperature (4) Optimum pH (5) Thermal stability (6) pH Stability (b) allowing an isomaltodextranase to act on the resulting mixture in the step (a) to form isomaltose; (c) hydrogenating either the resulting mixture in the step (b) directly or the isomaltose, which has been separated from the mixture to form isomaltitol; and (d) collecting the formed isomaltitol.