Patent ID: 8372959
Filing Date: 2013-02-12
Classification: C07K,C12N,C12P

Abstract:
1. A method for the production of a heterologous polypeptide of interest with a homogenous N-terminus, using a fusion polypeptide comprising the heterologous polypeptide of interest and N-terminally thereto a polypeptide exhibiting autoproteolytic function, said method comprising the steps of a) binding of the fusion polypeptide in a soluble, autoproteolytically inactive form by an affinity chromatography system, b) refolding of the fusion polypeptide, thereby activating the autoproteolytic function of the fusion polypeptide and causing cleavage of the heterologous polypeptide of interest, and c) subsequently eluting the heterologous polypeptide of interest, wherein said steps are conducted on one affinity chromatography system wherein said method utilizes an oligopolypeptide ligand selected from the group consisting of: which selectively binds to the part of the fusion polypeptide exerting autoproteolytic function under chaotropic conditions.