Patent ID: 9217163
Filing Date: 2015-12-22
Classification: C12P,Y02E

Abstract:
1. A continuous process for production of butanol and acetone comprising: establishing and maintaining a culture in a fermentor, said culture comprising Clostridium beijerinckii ATCC 10132 and a nutrient medium comprising glucose, malt extract, and beef extract, wherein the concentration of the glucose in the nutrient medium is about 2%, the concentration of the malt extract in the nutrient medium is about 5%, and the concentration of the beef extract is about 5%; maintaining the concentrations of glucose, malt extract, beef extract and Clostridium beijerinckii ATCC 10132 in the fermentor at levels sufficient to maintain a continuous process; wherein the process is carried out without removing acetone or butanol from the fermenter; wherein the process is carried out at a temperature between 33° C. and 39°, at a pH in the range of 5.0 to 7.0 and with an inoculum density in the range of 1 to 2%; and wherein the process results in a yield of at least 20 g/L of butanol and the Clostridium beijerinckii ATCC 10132 tolerating a butanol concentration of about 2.5% in the nutrient medium.