Patent ID: 9074223
Filing Date: 2015-07-07
Classification: C07K,C12N

Abstract:
1. A method for producing a protein of interest, comprising: (a) transducing in vitro a plurality of peripheral blood B cells with a retroviral vector pseudotyped with measles virus glycoproteins H and F to obtain a plurality of transduced B cells, wherein said retroviral vector comprises a nucleic acid of interest that encodes the protein of interest, the nucleic acid of interest being operably linked to a heterologous promoter that is capable of directing expression of the nucleic acid of interest in a plasma cell; (b) activating the plurality of transduced B cells to obtain a cell population comprising transduced B cells that have differentiated into CD38+ plasma cells, said step of activating comprising contacting the B cells with a composition comprising CD40L in combination with a B cell activating factor comprising one or more factors selected from IL-10, IL-4, IL-7, CpG DNA, IL-2, IL-15, IL-6, and IFN-α, wherein one of said factors is IL-15; and (c) culturing the CD38+ plasma cells of (b) so that the heterologous promoter directs expression of the nucleic acid of interest, and thereby producing the protein of interest.