Patent ID: 7645570
Filing Date: 2010-01-12
Classification: A61P,G01N

Abstract:
1. A method of detecting Botulinum toxin serotype A (BoNT/A) activity, the method comprising the steps of: a. contacting a sample to a cell that contains an exogenous Fibroblast Growth Factor Receptor 3(FGFR3), an exogenous synaptic vesicle glycoprotein 2(SV2), and a synaptosome-associated protein of 25000 Daltons (SNAP-25), wherein said cell is genetically engineered to express a nucleic acid molecule encoding said FGFR3 and a nucleic acid molecule encoding said SV2; wherein said cell is capable of BoNT/A intoxication; and b. detecting the presence of BoNT/A activity of said cell relative to a control cell, wherein the presence of SNAP-25 cleavage product from said cell is indicative of BoNT/A activity.