Patent ID: 8546108
Filing Date: 2013-10-01
Classification: C07K,C12N,C12P

Abstract:
1. An intracellular method of converting a single-chain Clostridial toxin comprising a di-chain loop region into its di-chain form, the method comprising the steps of: a) growing a cell comprising a dual expression construct at 37° C. for about 3.5 hours, the dual expression construct comprising; b) growing the cell at 22° C. for about 16 to about 18 hours, wherein growth at step (b) induces expression of the single-chain Clostridial toxin and the TEV protease from the dual expression construct; and wherein the produced TEV protease cleaves the single-chain Clostridial toxin at the TEV protease cleavage site located within the di-chain loop region, thereby converting the single-chain Clostridial toxin into its di-chain form.