Patent ID: 6394812
Filing Date: 2002-05-28
Classification: A61K,A61P,C12N

Abstract:
A hepatocyte cell culture for use in treating a patient in need of liver assist, comprising:one or more hepatocytes having increased detoxification enzyme activity isolated from a porcine donor liver, wherein, prior to isolating the hepatocytes from the donor liver, the porcine donor was administered at least one induction agent in an amount sufficient to increase detoxification enzyme activity of the hepatocyte, wherein the induction agent is selected from the group consisting of: beta-naphthoflavone, phenobarbital, 3-methylcholanthrene, ethanol, dexamethasone, arochlor 1254, 2,3,7,8-tetrachlorodibenzo-p-dioxin, phenothiazine, chlorpromazine, isosafole, &ggr;-chlordane, allylisopropylacetamide, trans-stilbene oxide, kepone, acetone, isoniazid, pyridine, pyrazole, 4-methylpyrazole, pregnenolone 16&agr;-carbonitrile, troleandomycin, clotrimazole, clofibrate, clobuzarit, di(2-ethylhexyl)phthalate, and mono-(2-ethylhexyl)phthalate.