Patent ID: 6569618
Filing Date: 2003-05-27
Classification: C12Q

Abstract:
A process for screening for a gene involving a coronary artery spasm-associated disease, which comprises;obtaining a DNA sample from a subject to be tested for a coronary artery spasm-associated disease; and detecting the presence of one or more nucleotide changes in a gene encoding human endothelial nitric oxide synthase (eNOS), which changes are selected from a group consisting of the changes in the eNOS cDNA sequence from guanine (G) to thymine (T) at position 894, and from cytosine (C) to thymine (T) at position 774 of SEQ ID NO:2, and the changes in the 5â€² flanking region of the human eNOS gene from thymine (T) to cytosine (C) at position 815, from adenine (A) to guanine (G) at position 679, and from thymine (T) to adenine (A) at position 133 of SEQ ID NO:1, and changes at the corresponding positions in the complementary strands thereof.