Patent ID: 6893850
Filing Date: 2005-05-17
Classification: B01J,B82Y,C12M,C12N,C40B

Abstract:
1. A method for patterning cells, comprising: providing a masking system, the masking system comprising a flexible cohesive mask, and the masking system including a first surface, an opposing second surface, and a plurality of channels passing through the masking system connecting the first surface with the second surface; pre-coating a second agent onto the second surface and the plurality of channels of the masking system, wherein the first surface of the masking system is free of the second agent; thereafter, shielding a first portion of a surface of an article with the masking system by contacting the first surface of the masking system with the article, wherein the flexible cohesive mask is in conformal contact with the surface of the article; applying a first agent through a channel within the masking system to a second portion of the surface of the article while preventing application of the first agent to the first portion of the surface of the article, the channel being one of the plurality of channels; thereafter, applying cells through the channel within the masking system to the second portion of the surface of the article so the cells contact the first agent while preventing application of the cells to the first portion of the surface of the article; and removing the masking system from the first portion of the surface of the article.