Patent ID: 6127151
Filing Date: 2000-10-03
Classification: A61K,A61P,C07K,C12N,Y10S

Abstract:
A method of deriving an antigen from Bordetella pertussis, wherein the antigen is characterized as (i) having a relative molecular weight of about 67,000 to 73,000 Daltons as determined by 12% (w/w) polyacrylamide gel electrophoresis and (ii) is recognized by monoclonal immunoglobulin secreted by the hybridoma deposited at the European Collection of Animal Cell Cultures, Porton Down, United Kingdom, under accession number 90010501, which method comprises:(a) culturing Bordetella pertussis cells;(b) treating the culture of step (a) to obtain an outer membrane fraction;(c) isolating an antigenic preparation comprising the antigen from the outer membrane fraction of step (b); and(d) recovering the antigenic preparation comprising the antigen from step (c); andwherein the antigenic preparation contains only minor quantities of any other antigens of Bordetella pertussis.