Patent ID: 8101741
Filing Date: 2012-01-24
Classification: A61K,C12N

Abstract:
1. A modified siRNA comprising a double-stranded region of 19 to 25 nucleotides in length, wherein from 20% to 30% of the nucleotides in the double-stranded region comprise 2′-O-methyl (2′OMe) nucleotides, wherein the modified siRNA comprises 2′OMe nucleotides in both strands of the modified siRNA, wherein the modified siRNA comprises at least one 2′OMe-guanosine nucleotide and at least one 2′OMe-uridine nucleotide in the double-stranded region, wherein 2′OMe-guanosine nucleotides and 2′OMe-uridine nucleotides are the only modified nucleotides present in the double-stranded region, wherein the modified siRNA is chemically synthesized, wherein the modified siRNA is less immunostimulatory than a corresponding unmodified siRNA sequence, and wherein the modified siRNA is capable of silencing expression of a target sequence.