Patent ID: 6077674
Filing Date: 2000-06-20
Classification: B01J,C07H,C12N,C40B

Abstract:
A method of making a full length oligonucleotide array from a mixture of pre-synthesized oligonucleotides on a support, the mixture comprising full-length oligonucleotide and shorter-length oligonucleotide each having a 5'-end and a 3'-end, a free-end of the full-length oligonucleotide having a protecting group and a free-end of the shorter-length oligonucleotide having a capping group, the other-end of each oligonucleotide being the linked end that is attached to the support, the method comprising the steps of:reacting a linking agent with the full-length oligonucleotide to couple a linking group to the free-end of the full-length oligonucleotide;cleaving the other-end of the oligonucleotides in the mixture from the support; anddepositing without separately purifying the oligonucleotide mixture on a surface of an array substrate for attachment to the surface, the linking group on the free-end of the full-length oligonucleotide preferentially attaching to the surface of the substrate over other groups present on the full-length and shorter length oligonucleotides of the mixture thereby forming the full length oligonucleotide array.