Patent ID: 9212387
Filing Date: 2015-12-15
Classification: C12Q

Abstract:
1. A detection method for the presence of an RNA of target genes of microRNA in a cell extract, wherein the RNA of target genes of microRNA comprises mRNA, non-mRNA, or a combination thereof of the target genes of microRNA, said method comprising: adding a labeled microRNA to a cell extract under conditions sufficient to form a complex between the labeled microRNA and the RNA of target genes of microRNA when the RNA of target genes of microRNA is present in the cell extract, wherein the labeled microRNA is a double-stranded microRNA with a labeled sense strand, and wherein said complex comprises an RNA-induced silencing complex (RISC), and detecting the presence of the RNA of target genes of microRNA in the cell extract by analyzing for formation of the complex between the labeled microRNA and the RNA of target genes of microRNA.