Patent ID: 6037126
Filing Date: 2000-03-14
Classification: C12Q,G01N

Abstract:
A first ribonucleic acid (RNA) molecule and a second RNA molecule for use in determining the presence or absence of a first subunit protein component of a telomerase molecule, said first RNA molecule binding said first subunit protein component under binding conditions and having the following formula:5'--A--B--C--3'wherein A is a section of the RNA molecule having 10-100,000 nucleotides which section is, with another RNA sequence, F, replicated by an RNA replicase, the letter ""B"" denotes a section of the RNA molecule having approximately 10 to 250 nucleotides comprising sequences from the Y region of human telomerase which section specifically binds to said first subunit protein component of under binding conditions, and the letter ""C"" denotes a section of the RNA molecule having approximately 1 to 10,000 nucleotides which section is ligated to another RNA sequence, ""D""; under ligation conditions, said second RNA molecule binding said first subunit protein molecule under binding conditions and having the following formula:5'--D--E--F--3'wherein D is a section of the RNA molecule having approximately 1 to 10,000 nucleotides which section is ligated with another RNA sequence, ""C"", under ligation conditions, the letter ""E"" denotes a section of the RNA molecule having approximately 10 to 250 nucleotides comprising sequences from the Y region of human telomerase which section specifically binds said first subunit protein component under binding conditions, and the letter ""F"" denotes a section of the RNA molecule having 10 to 100,000 nucleotides which section is, with another sequence, ""A"", replicated by said RNA replicase; said the first and the second RNA molecules forming a third RNA molecule upon ligation of sections C and D, which third RNA molecule has the following formula:5'--A--B--C--D--E--F--3'said third RNA molecule formed by ligating the C and D sections, as the E and the B sections are bound to said first subunit protein component, said third RNA for being replicated by said RNA replicase as an indication of the presence or absence of the first subunit protein component.