Patent ID: 7541515
Filing Date: 2009-06-02
Classification: C12N

Abstract:
1. A method of increasing expression of a heterologous protein in plant seed, comprising (a) introducing a recombinant nucleotide sequence encoding the protein operably linked to a promoter that is preferably expressed in plant seed tissue or expresses in seed as well as in other plant tissue into a maize plant, wherein the maize plant produces seed having an oil content greater than about 5.1 percent on a dry weight basis, such that the ratio of heterologous protein to total protein measured on a dry weight basis in seed of said maize plant increases compared to a maize plant producing seed not having an oil content greater than about 5.1 percent on a dry weight basis; (b) harvesting seed of said maize plant wherein said seed has an oil content greater than about 5.1 percent on a dry weight basis.