Patent ID: 6391650
Filing Date: 2002-05-21
Classification: G01N,Y10T

Abstract:
A method for preparing a sample protein solution for electrophoresis analysis, said method comprising the steps of:a) preparing a column comprising a first end, a second end and a size exclusion material wherein said size exclusion material is particulate and excludes proteins but not solvents, by equilibrating said size exclusion material with a first solvent; b) introducing a reagent into said first end of said column to create a reagent zone, wherein said reagent zone has a sufficient volume, length and size to allow a reaction to occur while proteins are passing through the reagent zone; c) introducing a protein sample into said first end of said column to create a sample zone, wherein said sample contains a plurality of protein molecules; d) allowing said protein molecules to flow through said column, wherein said protein molecules pass through said reagent zone, and pass into said first solvent while in said column; and e) collecting said protein molecules from said second end of said column.