Patent ID: 6635449
Filing Date: 2003-10-21
Classification: A61K,C07K,C12N,C12Q,C12Y

Abstract:
A method for producing a mutagenized progeny polynucleotide, comprising:(a) subjecting a starting or parental polynucleotide set to an in vitro exonuclease-mediated reassembly process, said exonuclease-mediated reassembly process comprising treatment with an enzyme having a 3â€² exonuclease activity, treatment with an enzyme having a 5â€² exonuclease activity, treatment with an enzyme having both a 3â€² exonuclease activity and a 5â€² exonuclease activity, treatment with an enzyme having nuclease activity, treatment with an enzyme having polymerase activity, or a combination thereof, whereby single-stranded polynucleotide sequences are generated; and (b) producing a progeny polynucleotide resulting from hybridization of the single-stranded polynucleotide sequences of (a).