Patent ID: 6204024
Filing Date: 2001-03-20
Classification: C12Q

Abstract:
A method of genotyping the CCR5 chemokine receptor to determine whether an individual is homozygous mutant, heterozygous or homozygous wild type, comprising:(a) providing CCR5 transcript RNA of an individual to be genotyped, wherein said CCR5 transcript RNA is obtained from whole blood or peripheral blood mononuclear cells of the individual;(b) amplifying said CCR5 transcript RNA using an isothermal transcription based amplification method; and(c) determining whether an amplification product of step (b) contains a wild type CCR5 allele, a mutant CCR5 allele or both wild type and mutant CCR5 alleles by independently hybridizing amplification product with labeled wild type and mutant specific probes, and detecting the resulting hybridization products.