Patent ID: 6399315
Filing Date: 2002-06-04
Classification: A61K,C07K

Abstract:
A method for screening a collection of compounds to determine those compounds which bind to corticotropic-releasing factor (CRF) receptor(s), said method comprising:(i) contacting transfected cell(s) with at least one compound whose ability to bind CRF receptor protein is sought to be determined, wherein said cell(s) is (are) transfected with nucleic acid encoding a mammalian G protein-coupled CRF receptor protein, wherein said nucleic acid: (a) encodes the amino acid sequence set forth in SEQ ID NO:2, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, or SEQ ID NO:15, (b) encodes the amino acid sequence encoded by the CRF-R encoding portion of clone hctCRFR, deposited with the ATCC under accession number 75474; or (c) is naturally occurring nucleic acid that hybridizes to the nucleic acid of (a) or (b) under the following conditions: 50% formamide, 5Ã—Denhart's solution, 5Ã—SSPE, 0.2% SDS, at 42Â° C., followed by washing in 0.2Ã—SSPE, 0.2% SDS, at 65Â° C., â€ƒwherein said naturally occurring nucleic acid encodes CRF-R that binds CRF, or nucleic acid degenerate to said naturally occurring nucleic acid; and (ii) monitoring said cell(s) for compounds which bind said CRF receptor(s).