Patent ID: 8389804
Filing Date: 2013-03-05
Classification: C12N

Abstract:
1. A method of alleviating superinfection exclusion of Citrus tristeza virus (CTV) viral vectors associated with successive transfections of viruses to a target plant, said method comprising: inoculating the target plant with a first CTV viral vector having a p33 gene omitted therefrom or which does not encode a functional p33 protein; allowing the first CTV viral vector to infect the target plant thereby to produce a primarily infected plant, and inoculating the primarily infected plant with a second CTV viral vector that either comprises or does not comprise a p33 gene or a p33 gene which does not encode a functional protein, whereby the second CTV viral vector further infects the primarily infected plant to produce a secondarily infected plant, wherein the second CTV vector is engineered to include an expressible sequence encoding a heterologous protein; and wherein the first CTV viral vector and the second CTV viral vector are of the same strain.