Patent ID: 7074561
Filing Date: 2006-07-11
Classification: C12Q,Y10S

Abstract:
1. A method for the detection or quantitation of alpha-fetoprotein (AFP) mRNA in a sample, comprising: (a) obtaining a sample which may contain AFP mRNA; (b) performing isothermal transcription based amplification on the sample with two oligonucleotide primers, a first primer which comprises at least 10 consecutive nucleotides of a sequence selected from the group consisting of SEQ ID NO: 2 and SEQ ID NO: 3, and a second primer which comprises at least 10 consecutive nucleotides of a sequence selected from the group consisting of SEQ ID NO: 6 and SEQ ID NO: 7; and (c) detecting or quantitating the amplification product of step (b) whereby detection or quantitation of the amplification product indicates the presence or quantity of AFP mRNA in the sample.