Patent ID: 8927248
Filing Date: 2015-01-06
Classification: C12N,C12Y,D21C,D21H

Abstract:
1. A modified Family 11 xylanase comprising a substituted amino acid at position 40 selected from the group consisting of arginine, cysteine, phenylalanine, and histidine, and an intramolecular disulfide bond produced by substitution of an amino acid at position 99, 118 or both positions 99 and 118 with a cysteine, said positions determined from sequence alignment of said modified Family 11 xylanase with the Trichoderma reesei xylanase II amino acid sequence of SEQ ID NO:16, wherein said modified Family 11 xylanase exhibits a. increased thermophilicity, alkalophilicity, thermostability, or a combination thereof, relative to a parental Family 11 xylanase from which the modified Family 11 xylanase is derived, and b. at least 92% amino acid sequence identity to SEQ ID NO: 16.