Patent ID: 8907062
Filing Date: 2014-12-09
Classification: C12N,C12Y

Abstract:
1. A method of producing and purifying recombinant mitochondrial targeting signal (MTS)-truncated coenzyme Q5 homolog (COQ5) protein in non-denaturing conditions, comprising the steps of: providing an inducing the expression of the recombinant COQ5 protein in the lysing the bacterial cells with a lysis buffer comprising lysozyme and deoxyribonuclease I (DNase I); pre-treating the bacterial cell lysate with less than 0.2% of an ionic detergent; centrifuging the cell lysate for removing insoluble proteins or debris and collecting the supernatant containing the recombinant COQ5 protein; and applying the supernatant to nickel-nitrilotriacetic acid (Ni-NTA) beads under native conditions that lack exposure to urea to obtain purified recombinant COQ5 protein.