Patent ID: 6716579
Filing Date: 2004-04-06
Classification: C12Q

Abstract:
An array comprising a plurality of polynucleotide probes immobilized on a solid support, wherein:(a) the plurality of polynucleotide probes corresponds to a multiplicity of gene transcripts and comprises at least 100 polynucleotides that are each complementary to a distinct gene transcript; (b) each polynucleotide probe of the plurality is localized to a predetermined region on the solid support; (c) each polynucleotide probe of the plurality is from about 50 to 500 nucleotides in length; (d) each polynucleotide probe of the plurality is complementary to 3â€² untranslated sequence of a gene transcript, the untranslated sequence having a defined chromosomal location; and (e) each polynucleotide probe of the plurality is prepared by amplification of genomic DNA or cDNA using a primer pair selected from the group consisting of SEQ ID NOs. 1-2, 3-4, 5-6, 7-8, 9-10, 11-12, 13-14, 15-16, 17-18, 19-20, 21-22, 23-24, 25-26, 27-28, 29-30, 31-32, 33-34, 35-36, 37-38, 39-40, 41-42, 43-44, 45-46, 47-48, 49-50, 51-52, 53-54, 55-56, 57-58, 59-60, 61-62, 63-64, 65-66, and 67-68.