Patent ID: 6546123
Filing Date: 2003-04-08
Classification: G01N,G02B,G06K,G06T

Abstract:
A method for automated detection of a cell proliferative disorder, comprising,a) providing a sample on a slide, wherein the sample is contacted with an antibody that binds to a protein associated with a cell proliferative disorder; b) identifying a processing parameter for the sample of a); c) scanning the sample of b) at a plurality of locations at a low magnification on an optical system; d) acquiring a low magnification image at the low magnification at each location in the scanned area; e) transforming substantially all pixels of the image from a first color space to a second color space; f) processing each low magnification image by means of a computer processor to detect rare candidate objects of interest in said second color space with reference to a predetermined threshold; g) storing stage coordinates of each location for each candidate object of interest; h) adjusting the optical system to a higher magnification; i) repositioning the stage to the location for each candidate object of interest; j) acquiring a higher magnification image of each candidate object of interest; and k) storing each higher magnification image.