Patent ID: 6261768
Filing Date: 2001-07-17
Classification: C12Q

Abstract:
A method of detecting a genetic polymorphism in an individual, the method comprising the following steps:(1) obtaining a sample containing nucleic acid from the individual;(2) amplifying the nucleic acid sample from step (1) by a process involving thermocycling and primers, the amplification occurring in the presence of a thermostable restriction endonuclease which is active during thermocycling, the primers being selected such that they introduce a sequence recognized by the thermostable restriction endonuclease into the nucleic acid resulting from amplification of the sample nucleic acid not including the polymorphism, or from amplification of the sample nucleic acid not including the polymorphism amplified from nucleic acid including the polymorphism, wherein the sample nucleic acid is not amplified prior to step (2) and wherein during thermocycling the thermostable restriction endonuclease inhibits amplification of the nucleic acid which contains the sequence recognized by the restriction endonuclease and allows amplification of the nucleic acid which lacks the sequence recognized by the restriction endonuclease; and(3) analyzing the product of step (2) to determine the presence or absence of the polymorphism.