Patent ID: 6794135
Filing Date: 2004-09-21
Classification: C12Q,Y10T

Abstract:
A method of detecting extracellular 5T4 RNA in blood plasma or serum from a human for detecting, diagnosing, monitoring, treating, or evaluating a neoplastic disease comprising cells that express 5T4 RNA, the method comprising the steps of:(a) extracting heterogeneous human extracellular RNA from blood plasma or serum; (b) amplifying a portion of the extracted RNA or the corresponding cDNA to produce an amplified DNA fragment, wherein said portion comprises 5T4 RNA, and wherein amplification is performed in either a qualitative or quantitative fashion using primers or probes specific for 5T4 RNA or corresponding cDNA; and (c) detecting the amplified fragment produced from 5T4 RNA or corresponding cDNA.