Patent ID: 8304236
Filing Date: 2012-11-06
Classification: C07K,G01N

Abstract:
1. A modified tamavidin-2 biotin-binding protein, comprising an amino acid sequence having 80% or more identity to the sequence represented by SEQ ID NO: 2, wherein said modified biotin-binding protein having one or more amino acid residues replaced with an acidic amino acid residue or a neutral amino acid residue, said one or more amino acid residues selected from the group consisting of: the arginine residue at position 104 of SEQ ID NO: 2; the lysine residue at position 141 of SEQ ID NO: 2; the lysine residue at position 26 of SEQ ID NO: 2; and the lysine residue at position 73 of SEQ ID NO: 2, wherein said modified tamavidin-2 biotin-binding protein has biotin binding activity.