Patent ID: 8691507
Filing Date: 2014-04-08
Classification: A61K,A61P,C07D,C12Q,G01N

Abstract:
1. A method comprising: (i) providing a nucleic acid sample from a biological sample obtained from a subject; (ii) contacting the nucleic acid sample with at least one primer that specifically hybridizes to a portion of SEQ ID NO: 7 having a mutation at the nucleotides encoding position Tyr641 (Y641) of Enhancer of Zeste homolog 2 (EZH2) of SEQ ID NO: 1, wherein the mutation increases EZH2 trimethylation of Lys27 of histone H3 (H3-K27); (iii) detecting the presence of the mutation at the nucleotides encoding Y641 in the nucleic acid sample by detecting the presence of a nucleic acid encoding the mutation at Y641; (iv) identifying the subject as a candidate for treatment; and (v) administering a therapeutically effective amount of an EZH2 inhibitor to the subject identified in step (iv), wherein the EZH2 inhibitor inhibits the conversion of H3-K27 to trimethylated H3-K27.