Patent ID: 8968684
Filing Date: 2015-03-03
Classification: B01L,G01N

Abstract:
1. A method of facilitating a temperature controlled chemical reaction in a microplate comprising: a) providing a microplate comprising a top frame having a planar topside and a plurality of openings defining microwells that hang below and individually comprise side walls and a planar or slightly convex bottom surface, wherein the top frame has flexibility; b) providing at least one sample in at least one microwell of said microplate; c) sealing off the at least one microwell; d) providing a first heating/cooling platen having a temperature control and a rigid planar top surface lacking cavities configured to receive the microwells; e) adjusting the temperature control to reach a desired temperature on the top surface of the first heating/cooling platen; f) engaging said bottom surfaces of the microplate with the heating/cooling platen's rigid top surface; g) providing a means for clamping configured to provide a downward vertical force and applying a vertical load on the topside of the top frame of the microplate, such that the bottom surfaces of the microwells are held flat or substantially so against the rigid planar top surface; and h) holding the means for clamping to the microplate assembly for a specific period of time to allow the temperature controlled chemical reaction with the sample to take place.