Patent ID: 6150511
Filing Date: 2000-11-21
Classification: C07K,C12N

Abstract:
A nucleic acid molecule encoding a chimeric enzyme comprising a first segment derived from a first source operably linked to a second segment derived from a second source, said second source being different from said first source, said first segment encoding a DNA binding moiety and said second segment encoding an integrase moiety derivable from a retroelement, said integrase moiety having a carboxy terminus and an amino terminus, said amino terminus having a ZF domain, wherein said integrase moiety is truncated from said amino terminus so as to remove part or all of said ZF domain, and said DNA biding moiety is appended to said integrase moiety at said truncated amino terminus, said enzyme binding specifically to a targeted host DNA molecule having a characteristic determinant recognized by said DNA binding moiety, and possessing integrase activity, whereby said enzyme catalyzes site-specific integration of foreign DNA into a host genome sequence.