Patent ID: 8153424
Filing Date: 2012-04-10
Classification: C12N,G01N

Abstract:
1. A method of creating a population of human ventral spinal progenitor cells, the method comprising the steps of: (a) obtaining a synchronous population of cells that are cultured from human embryonic stem cells, that are characterized by an early rosette morphology and that are Pax6 (b) culturing the cells of step (a) with a sufficient amount of retinoic acid in adherent culture, wherein the resulting cells are positive for Hoxb4 and are negative for at least one of Otx2 or Bf1; (c) expanding the resulting cells of step (b) in a suspension culture with a sufficient amount of retinoic acid; and (d) culturing the cells of step (c) in a suspension culture with a sufficient amount of retinoic acid and an agent selected from the group consisting of sonic hedgehog and purmorphamine, wherein at least 80% of the resulting cells are Olig2