Patent ID: 6057119
Filing Date: 2000-05-02
Classification: C07K,C12N,C12Q,G01N

Abstract:
A method for identifying a potential inhibitor of a mutant interleukin-1.beta. converting enzyme, the method comprising the steps of:a. using a three-dimensional structure of interleukin-1.beta. converting enzyme as defined by atomic coordinates of interleukin-1.beta. converting enzyme according to FIG. 5;b. replacing one or more interleukin-1.beta. converting enzyme amino acids selected from 150,151,173,176,177,178,179,180,236,237,238,239,240,244, 248,259,267,268,274,283,284,285,290,291,292,293,294,295,296,297,317,318, 319,320,321,322,323,324,325,327,334,335,338,339,340,341,342,343,345,348, 352,367,371,374,375,377,378,380,381,382,383,384,386,388,389,390,391,392, 393,394,395 or 396 of SEQ ID NO;1 in said three-dimensional structure with a different a naturally occuring amino acid;c. employing said three-dimensional structure to design or select said potential inhibitor;d. synthesizing said potential inhibitor; ande. contacting said potential inhibitor with said mutant interleukin-1.beta. converting enzyme or said interleuldn-1.beta. converting enzyme in the presence of a substrate to test the ability of said potential inhibitor to inhibit said interleukin-1.beta. converting or said mutant interleukin-1.beta. converting enzyme.