Patent ID: 7364787
Filing Date: 2008-04-29
Classification: C07F,C07H,C12N,C12P

Abstract:
1. An isolated polynucleotide which is selected from the following polynucleotides (a) to (c) which encodes a polypeptide having sphingolipid ceramide N-deacylase activity: (a) a polynucleotide consisting of the nucleotide sequence of SEQ ID NO:2 or a portion thereof wherein said polynucleotide encodes a polypeptide having a sphingolipid ceramide N-deacylase activity; (b) a polynucleotide, which hybridizes to a complement of the polynucleotide or portion thereof of (a), when incubated for 4 hours to overnight at 65 degrees C in 6×SSC (1×SSC is a solution containing 0.15 M NaCI and 0.015 M sodium citrate having a pH value of 7.0) supplemented with 0.5% SDS, 5× Denhartz's (0.1% bovine serum albumin (BSA), 0.1% polyvinyl pyrrolidone, 0.1% Ficoll 400) and 100 μg/ml of salmon sperm DNA; and (c) a polynucleotide comprising the polynucleotide of any one of the above (a) and (b).