Patent ID: 7452674
Filing Date: 2008-11-18
Classification: C07H,C12Q

Abstract:
1. A method for detecting the presence or absence of a target nucleic acid in a test sample, comprising: a) contacting the test sample with a composition comprising an oligo- or poly nucleotide probe wherein the oligo- or polynucleotide probe comprises a detectable label of formula: base is wherein sugar is covalently bonded to N wherein R wherein R each of X, Y, and Z is independently —H, —OH, —O-alkyl, —SH, —SR wherein R R R linker is (CH wherein n is an integer from 1 to 30; Q is O, S, or NH; label is a colorimetric, chemiluminescent, bioluminescent, a fluorescent compound, or a non- or weakly fluorescent compound; wherein the oligonucleotide probe is capable of selectively or specifically hybridizing with the target nucleic acid under conditions of moderate stringency; optionally exposing the sample to light of said excitable wavelength; and b) detecting whether said contacting produces a change in color or fluorescence emission of the composition; such that a change in color or fluorescence emission of the composition indicates that the target nucleic acid is present in the test sample.