Patent ID: 8975056
Filing Date: 2015-03-10
Classification: A21D,C12N,C12P,G01N,Y02E

Abstract:
1. An isolated variant polypeptide having alpha-amylase activity and at least one altered characteristic that improves enzyme performance, the variant polypeptide comprising: an amino acid sequence having at least 90% amino acid sequence identity to a parental alpha-amylase polypeptide selected from AmyE (SEQ ID NO: 1) or at least 92% amino acid sequence identity to a truncated variant of AmyE (SEQ ID NO: 2), and a modification at one or more positions selected from the group consisting of 35, 56, 65, 158, 189, 192, 298, 320, 386 and 395, wherein the modification produces a variant polypeptide having a performance index (PI) greater than 1.0 for at least one characteristic that improves enzyme performance, and wherein the modification at position 386 consists of the amino acid residue substitution A386R.