Patent ID: 6962804
Filing Date: 2005-11-08
Classification: A01K,C12N,G01N,G16B

Abstract:
1. A method for generating a Drosophila clipped FRT (cFRT) chromosome incapable of reacting with a P transposase but capable of reacting with a yeast site-specific flippase recombinase (FLP), comprising steps of: (a) obtaining a first FRT chromosome by causing a local and random transposition by exposing a FRT chromosome to said P transposase, wherein said FRT chromosome contains a P[FRT] insertion with a selection marker gene; (b) obtaining a second FRT chromosome by screening for said first FRT chromosome lacking said selection marker gene; (c) selecting a third FRT chromosome from said second FRT chromosome by the steps of: (d) exposing said third FRT chromosome to said P transposase and obtaining said