Patent ID: 6313264
Filing Date: 2001-11-06
Classification: A61K,A61P,C07K,C12N,G01N

Abstract:
An isolated polypepbide having a molecular weight of about 210 kDa as determined by SDS-PAGE, the polypeptide made by a process comprising the steps of:(a) providing a sample of human cellular material;(b) preparing an extract of the cellular material comprising cell membrane proteins,(c) contacting the extrct with an affinity reagent comprising a complex of:(i) rapamycin, and(ii) FKBP12under conditions which permit materials capable of specifically binding to the affinity reagent to bind thereto;(d) separating materials which do not bind to the affinity reagent from the affinity reagent and the materials bound thereto;(e) dissociating the materials bound to the affinity reagent therefrom; and(f) separating the polypeptide having a molecular weight of about 210 kDa from the other materials dissociated from the affinity reagent.