Patent ID: 6180411
Filing Date: 2001-01-30
Classification: C07D,G01N,Y10S

Abstract:
A compound comprising:a chromophore incorporating a photolabile group capable of undergoing an irreversible and detectable intramolecular chemical transformation upon irradiation by light, the chromophore being linked to a binding site capable of binding an analyte,the compound having a structure, represented by an azido-substituted aromatic ring A with additional substituents Y and Z and represented by the formula: ##STR5##and the pharmaceutically acceptable salts and esters thereof wherein:wherein X is O and Y is a fused BAPTA or BAPTA-like derivative and is fused or attached to a single atom of A;Z is a substituent attached to A ortho to the azido group and is represented by R--C(O)--,wherein R is independently a hydrocarbyl radical or an heterocyclic aromatic radical,wherein the photolabile group comprises a .beta.-azido, .alpha.,.beta.-unsaturated carbonyl group and the chemical transformation is conversion of the .beta.-azido, .alpha.,.beta.-unsaturated carbonyl group to an isoxazole,wherein the analyte is a cation selected from the group consisting of Ca++, Na+, Mg++ and H+, andwherein binding of the analyte to the binding site alters an optical property of the chromophore, thus altering the ability of the photolabile group to undergo the chemical transformation upon irradiation.