Patent ID: 6171787
Filing Date: 2001-01-09
Classification: A61K,A61P,C07K,C12Q,G01N

Abstract:
A method of detecting the presence of a target polynucleotide of TNF-gamma in a test sample, comprising:(a) contacting said test samples, under conditions such that binding occurs, with at least one polynucleotide sequence corresponding to SEQ ID NO:1 or a full-length complement thereof, wherein said at least one polynucleotide sequence or said full-length complement thereof is conjugated to a detectable label capable of generating a measurable signal;(b) determining, subsequent to detection of said signal, whether at least one polynucleotide sequence of said test sample, which binds to said at least one polynucleotide sequence corresponding to SEQ ID NO:1 or said full-length complement thereof, has at least 70% identity to SEQ ID NO:1 or said full-length complement thereof,wherein the presence of said at least one polynucleotide sequence having at least 70% identity to SEQ ID NO:1 or said full-length complement thereof indicates target TNF-gamma polynucleotide in said test sample.