Patent ID: 9212356
Filing Date: 2015-12-15
Classification: C12N,C12P

Abstract:
1. A method of producing a diaminoalkylene-N,N′-disuccinate, the method comprising: reacting fumaric acid and a diamine in the presence of a host transformant or transductant comprising a recombinant expression vector comprising a gene encoding a protein having the amino acid sequence of SEQ ID NO: 1 or having an amino acid sequence derived from the amino acid sequence of SEQ ID NO: 1 by substitution of one to ten amino acid residues, wherein the protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity; and collecting the diaminoalkylene-N,N′-disuccinate resulting from the reaction products, wherein the protein has ethylenediamine-N,N′-disuccinate:ethylenediamine lyase activity that is heat resistant.