Patent ID: 8580487
Filing Date: 2013-11-12
Classification: A01N

Abstract:
1. A method of preserving a whole human ovary, comprising: a. freezing the whole human ovary including vasculature, by perfusing the ovary including vasculature with a solution comprising a cryoprotectant and placing the ovary in a solution comprising a cryoprotectant; moving the ovary through a predetermined temperature gradient, thereby creating a cooling rate in a range of from 0.03 to 1.5° C./minute; seeding the solution to propagate an ice interphase according to the freezing point of the solution; and storing the ovary including vasculature at a temperature below freezing temperature; and b. thawing the whole human ovary including vasculature, and removing the solution comprising a cryoprotectant, to obtain a thawed whole human ovary, such that after thawing at least a portion of cells contained therein resume biological activity and a blood vessel present in the vasculature is in a state to permit its supply with blood by a vasculature belonging to an ovary recipient, wherein the thawed whole human ovary including vasculature is suitable for transplantation and re-anastomosis in the ovary recipient.