Patent ID: 6379883
Filing Date: 2002-04-30
Classification: C12Q,Y10S

Abstract:
A method of measuring the concentration of an analyte in a sample that contains a co-reactant, the method comprising:(a) providing a sample suspected of containing an analyte of interest, the sample comprising a co-reactant; (b) contacting the sample with a layer comprising a microfabricated aperture and a layer comprising a catalyst, wherein the co-reactant contacts the catalyst by diffusing substantially freely through the layer comprising the microfabricated aperture, and the analyte contacts the catalyst substantially through the aperture; (c) obtaining at least one detectable product from a reaction between the analyte, the co-reactant and the catalyst; and (d) detecting the product with a sensor thereby producing an output that depends upon the concentration of the analyte in the sample.