Patent ID: 8178503
Filing Date: 2012-05-15
Classification: G16B

Abstract:
1. A method for decreasing the expression of a transcript, the method comprising the steps of: identifying one or more regions of interest in a sequence of the given transcript; sub-selecting, within the one or more identified regions of interest, a motif, wherein sub-selecting a motif comprises identifying one or more identically conserved copies of the motif in intergenic and intronic regions of a genome of interest for the given transcript and identifying one or more additional copies in untranslated and amino acid coding regions of one or more genes in the genome of interest, wherein sub-selecting a motif further comprises using a pattern discovery technique with a pre-determined parameter of minimum size of discovered pattern and a pre-determined parameter of minimum required number of discovered copies before a pattern can be deemed as identified; generating one or more derived sequences from the sub-selected motif; creating, from the one or more derived sequences, at least one instance of the corresponding molecule that the one or more derived sequences represent, wherein the at least one instance of the corresponding molecule comprises at least one interfering RNA molecule; and using the at least one interfering RNA molecule to decrease the expression of the given transcript.