Patent ID: 6287861
Filing Date: 2001-09-11
Classification: C07K,C12N,C12Q,C12Y,C40B

Abstract:
A method for evolving a polynucleotide for acquisition of a desired functional property, comprising:(1) providing a library of variant forms of a polynucleotide, some of which are components of a vector, and some of which are not components of a vector,(2) introducing the library of variant forms into a population of cells,(3) propagating the cells under conditions whereby recombination occurs between the variant forms that are components of a vector and the variant forms that are not components of a vector to generate recombinant forms of the polynucleotide;(4) selecting or screening the recombinant forms of the polynucleotide to identify at least one recombinant form of the polynucleotide having evolved toward a desired property.