Patent ID: 6322992
Filing Date: 2001-11-27
Classification: G01N,Y10S

Abstract:
A method for the determination of a specific binding ligand comprising:(A) contacting a fluid sample suspected of containing said specific binding ligand with an analytic element comprising:(i) a porous spreading zone, and(ii) at least one additional zone which is in fluid contact with said porous spreading zone, said element containing in at least one of said zones a vanadium bromoperoxidase-labeled ligand or ligand analog, and in a separate zone, an immobilized receptor that binds said ligand or ligand analog;(B) applying a wash solution to said element to bring about a separation of free vanadium bromoperoxidase-labeled ligand or ligand analog from vanadium bromoperoxidase-labeled ligand or ligand analog that is bound to said immobilized receptor, said wash solution comprising a composition having a pH of from about 6.5 to about 10, wherein said composition comprises:(a) a chemiluminescent signal-generating reagent which provides a signal in response to the catalytic activity of said vanadium bromoperoxidase, wherein said signal-generating reagent is a 2,3-dihydro-1,4-phthalazinedione derivative;(b) a halogen, pseudohalogen, halogen-providing source or pseudohalogen-providing source; and(c) a peroxide or a peroxide-generating reagent composition, and(C) detecting either the free or bound vanadium bromoperoxidase labeled ligand or ligand analog as a measure of said specific binding ligand.