Patent ID: 6987007
Filing Date: 2006-01-17
Classification: C12N,C12Y

Abstract:
1. A method for producing and purifying a target protein molecule comprising: (a) constructing a DNA expression vector which expresses a hybrid polypeptide in a transformed host cell, the hybrid polypeptide comprising the target protein molecule and a modified chitin binding domain (CBD) that has a specific and reversible affinity for a substrate under non-denaturing conditions, wherein the non-denaturing conditions are selected from the group consisting of non-denaturing ionic conditions, non-denaturing pH, and non-denaturing detergent concentration; (b) introducing the expression vector into an appropriate host cell and expressing the hybrid polypeptide; (c) contacting the hybrid polypeptide produced by the transformed cell with the substrate to which the CBD binds; and (d) recovering the hybrid polypeptide.