Patent ID: 8343727
Filing Date: 2013-01-01
Classification: C07K,G01N

Abstract:
1. A method of binding a protein to a carrier, which comprises: providing a biotin-bound carrier; preparing a fusion protein having the protein bound to a tamavidin; and binding the protein to the carrier via tamavidin-biotin bonds, wherein the tamavidin is selected from: (a) a protein consisting of an amino acid sequence sharing an identity of 90% or more with SEQ ID NO: 2 or SEQ ID NO: 4 and having biotin-binding activity; or (b) a protein consisting of the amino acid sequence of SEQ ID NO: 2 or SEQ ID NO: 4; or (c) a protein consisting of an amino acid sequence encoded by a nucleic acid hybridizable under stringent conditions (0.5% SDS, 65° C., 0.2×SSC) with a strand complementary to the base sequence of SEQ ID NO: 1 or SEQ ID NO: 3 and having biotin-binding activity.