Patent ID: 6638714
Filing Date: 2003-10-28
Classification: C12N,C12Q

Abstract:
A method for detecting the presence of Hepatitis C Virus (HCV) RNA in a biological sample, said method comprising:(A) performing a reverse transcription reaction using, as a template, RNA derived from said sample to produce HCV-specific reverse transcription products; (B) amplifying said reverse-transcription products using one or more pairs of oligonuclecotide primers specific for HCV to produce HCV-specific amplification products, â€ƒwherein said pairs are selected fom the group consisting of: (a) forward primer 5â€²-CAGAAAGCGTCTAGCCATGGCGTTAGTA-3â€² (C69F28)  and reverse primer 5â€²-CGGTTCCGCAGAGACCACTATGGCTCTC-3â€² (C133R26) ; and (b) forward primer 5â€²-GGGAGAGCCATAGTGGTCTGCGGAA-3â€² (C131F25)  and reverse primer 5â€²-CGGGGCACTCGCAAGCACCCTATCA-3â€² (C294R26) ; and (C) detecting said amplifcation products, wherein detection of said amplification products indicates the presence of HCV RNA in said sample.