Patent ID: 6306637
Filing Date: 2001-10-23
Classification: A61K,C07K,C12N

Abstract:
A method of isolating a mixture of glycoproteins of a virus belonging to the paramyxoviridae family, which comprises:growing a virus belonging to the paramyxoviridae family and selected from the group consisting of respiratory syncytial virus (RSV) and parainfluenza virus (PIV), in a culture medium which is substantially free from exogenous growth factors and serum proteins on a cell substrate which is readily acceptable for use in human vaccine production,isolating the virus from said culture medium,solubilizing the glycoproteins from said isolated virus, andisolating and co-purifying said solubilized glycoproteins by affinity chromatography wherein the solubilized glycoproteins initially are bound to lentil-lectin or concanavilin A and subsequently eluted using a competing sugar.