Patent ID: 7923600
Filing Date: 2011-04-12
Classification: C12N

Abstract:
1. A method for producing a transformed plant that does not contain a selectable marker in its genome, comprising (a) infecting plant cells with Agrobacterium containing a plasmid that comprises (i) a transfer-DNA that does not contain a selectable marker gene and (ii) an expression cassette, positioned outside of the transfer-DNA, for expressing a cytokinin biosynthetic gene; (b) culturing the cells on a hormone-free medium to produce shoots, or on medium which contains an amount of hormone that does not promote the regeneration of untransformed shoots but does improve regeneration of shoots produced through cytokinin biosynthetic gene expression; (c) identifying a shoot that has genomic DNA containing the transfer-DNA but not the cytokinin biosynthetic gene expression cassette; and (d) growing a plant from the identified shoot of (c), wherein the plant is a transformed plant that does not contain a selectable marker in its genome.