Patent ID: 8951774
Filing Date: 2015-02-10
Classification: C12N,C12P,C12Y,Y02E,Y02P

Abstract:
1. A recombinant microbial host cell comprising genes encoding an engineered isobutanol biosynthetic pathway comprising the following substrate to product conversions: i) pyruvate to acetolactate, (pathway step a) ii) acetolactate to 2,3-dihydroxyisovalerate, (pathway step b) iii) 2,3-dihydroxyisovalerate to α-ketoisovalerate, (pathway step c) iv) α-ketoisovalerate to valine, (pathway step h) v) valine to isobutylamine, (pathway step i) vi) isobutylamine to isobutyraldehyde, (pathway step j) and vii) isobutyraldehyde to isobutanol; (pathway step e) wherein the substrate to product conversions of i)-vi) are encoded by genes that are heterologous to said recombinant microbial host cell and wherein said microbial host cell produces isobutanol.