Patent ID: 8045165
Filing Date: 2011-10-25
Classification: G02B

Abstract:
1. A method for determining a focal position of an imaging device adapted to image a: biologic sample, which sample has a refractive index, comprising the steps of: disposing lenslets relative to a field of a biologic sample such that the lenslets are substantially positionally fixed relative to the sample, which lenslets have a height, and have a refractive index and which refractive index is different from that of the sample; wherein one or both of the imaging device and the lenslets are relatively locatable so the focal position of the imaging device can be moved along the height of the lenslets; imaging at least the field of the sample and a plurality of the lenslets using transmittance at one or more predetermined wavelengths; determining a representative light transmittance intensity of the sample field at the wavelengths; determining a representative light transmittance intensity of at least one region of the lenslets at the wavelengths; and determining the focal position of the imaging device using the representative light transmittance intensity of the sample field and the representative light transmittance intensity of the region of the lenslets.