Patent ID: 8568709
Filing Date: 2013-10-29
Classification: A61K,A61P,C07H,C07K,C12N,C12Y

Abstract:
1. A composition comprising: a stably integrating delivery vector; a modified human thymidylate kinase (tmpk) polynucleotide that encodes a modified human tmpk polypeptide that increases phosphorylation of AZT relative to phosphorylation of AZT by wild type human tmpk polypeptide; a detection cassette polynucleotide that encodes a detection cassette polypeptide that is cell surface expressed, wherein the detection cassette polynucleotide is fused to the modified human tmpk polynucleotide and the detection cassette polypeptide is fused to the modified human tmpk polypeptide; and a therapeutic polynucleotide cassette, wherein the modified human tmpk polypeptide comprises a modification selected from (a) a F to Y mutation at amino acid position 105 of SEQ ID NO: 11, (b) a R to G point mutation at amino acid position 16 of SEQ ID NO: 12, or (c) a R to A mutation at amino acid position 200 of SEQ ID NO: 16.