Patent ID: 8932868
Filing Date: 2015-01-13
Classification: B01L,C12Q,G01N,G05B,Y10T

Abstract:
1. A method for the detection and/or allocation of at least one point mutation in target DNA and/or RNA duplexes, said method comprising at least the steps of: obtaining a functionalized surface which is coated with probe DNA and/or RNA whereto the target DNA and/or RNA duplexes are attached, contacting said functionalized surface to an electrolytic solution having a neutral pH in a flow cell and measuring a first impedance value within said electrolytic solution, and then adding a chemical to the electrolytic solution which is able to achieve denaturation of the target DNA and/or RNA, and then measuring a second impedance value within the flow cell after completion of the denaturation of the DNA and/or RNA target, and then obtaining a value representative for the impact of the chemical on the impedance of the electrolytic solution, and then determining the amount and/or allocation of point mutation(s) within the target DNA and/or RNA by calculating the denaturation-time constant based on the difference between the first and second impedance value and corrected for the impact of the chemical by the value representative for such impact.