Patent ID: 8473216
Filing Date: 2013-06-25
Classification: C12Q

Abstract:
1. A method of performing baseline correction for amplification curves in a PCR experiment, the method comprising: providing a processor; receiving a plurality of amplification curves for a sample, wherein the plurality of amplification curves are characterized by a baseline drift, each of the plurality of amplification curves being associated with a sample and one of a plurality of reagents; computing, using the processor, a first parameter for each of the plurality of amplification curves; computing, using the processor, a slope value for each of the plurality of amplification curves; computing, using the processor, a third parameter equal to an average of the slope values associated with each of the plurality of amplification curves; computing, using the processor, a baseline correction value for each of the plurality of amplification curves, the baseline correction value being a function of the first parameter and the third parameter; and performing baseline correction, using the processor, for at least one of the plurality of amplification curves by subtracting the baseline correction value.