Patent ID: 6558910
Filing Date: 2003-05-06
Classification: C07K

Abstract:
A method for identifying a compound that modulates taste signaling in taste cells, the method comprising the steps of:(i) contacting the compound with a taste transduction G-protein coupled receptor polypeptide, wherein the polypeptide is expressed in a taste cell, wherein the polypeptide binds to a 6-n-propylthiouracil ligand, and wherein the polypeptide is encoded by a nucleic acid that specifically hybridizes under stringent hybridization conditions to a nucleic acid comprising a nucleotide sequence of SEQ ID NO:42; wherein the hybridization reaction is incubated at 42Â° C. in a solution comprising 50% formamide, 5x SSC, and 1% SDS and washed at 65Â° C. in a solution comprising 0.2x SSC and 0.1% SDS; and (ii) determining whether said compound binds to and/or affects the function of said receptor polypeptide.