Patent ID: 6383740
Filing Date: 2002-05-07
Classification: G01N,Y10S

Abstract:
A method for simultaneously measuring both members A and B in a binding pair complex in a biological sample, said method comprising:a) providing a solid phase reagent, said solid phase reagent comprising a particle coated with capture antibodies having specific binding affinities for said member A of said binding pair complex; b) contacting said biological sample with said solid phase reagent under conditions in which said member A, if present, becomes bound to said particle, to form a first reacted particle: c) contacting said first reacted particle with first antibodies having specific binding affinities for said member A, wherein said first antibodies are labeled with a first label, and with second antibodies having specific binding affinities for said member B of said binding pair complex, wherein said second antibodies are labeled with a second label, to form a second reacted particle, wherein said first and second labels are different and d) measuring said first and second labels on said second reacted particle using flow cytometry.