Patent ID: 6471866
Filing Date: 2002-10-29
Classification: B01D,B01J,C12N,G01N

Abstract:
A method for separating a mixture of polynucleotide fragments comprisinga) applying a solution of said fragments and counterion reagent to a column containing separation media having a non-polar surface, wherein said separation media have a pore size greater than 30 Angstroms and an average diameter of 1-100 microns; b) eluting said fragments with a gradient eluting solvent of increasing organic component concentration containing a counterion agent; wherein surfaces which are contacted by the solution of the fragments and the eluting solvent are materials which do not trap or release multivalent metal cations therefrom, wherein said multivalent metal cations comprise multivalent metal cations which are free to interfere with polynucleotide separation.