Patent ID: 7723571
Filing Date: 2010-05-25
Classification: C07K,C12N

Abstract:
1. A method of expressing a small peptide in host endosperm cells, comprising the steps of: (i) providing an endosperm-specific promoter and a DNA leading sequence encoding an endosperm-specific signal peptide; (ii) providing a polynucleotide encoding a peptide consisting of a C terminus end of protein disulfide bond dismutase (PDI) as fusion carrier, wherein said C-terminus end possesses molecular chaperone activity; (iii) providing a target nucleic acid encoding the small peptide, wherein the small peptide is between 20 and 100 amino acids in length; (iv) constructing an expression vector comprising the promoter, the DNA leading sequence, the polynucleotide encoding the fusion carrier and the target nucleic acid encoding the small peptide wherein the target nucleic acid encoding the small peptide is fused to the 3′ end of the polynucleotide encoding the fusion carrier, wherein a fusion protein expressed from said expression vector comprises the small peptide fused to the C-terminus of said fusion carrier; (v) transforming a plant cell with the expression vector; and (vi) regenerating a mature plant from the transformed cell, such that seeds comprising endosperm cells are produced in which the peptide is expressed.