Patent ID: 6322727
Filing Date: 2001-11-27
Classification: C07D,C07F,C07H,C12Q,G01N

Abstract:
A kit for detecting a first substance in a sample, comprising:(1) a stabilized 1,2dioxetane bearing an enzyme-labile substituent, which is destabilized and caused to decompose by contacting said 1,2-dioxetane with an enzyme under conditions which cause said enzyme to cleave said enzyme-labile group from said dioxetane, yielding a negatively charged oxygen anion bonded to said 1,2-dioxetane, which causes said 1,2-dioxetane to decompose without input from an external excitation energy source, said decomposition being accompanied by chemiluminescence; and(2) a second component selected from the group consisting of said enzyme and said enzyme conjugated to a specific affinity substance;wherein said specific affinity substance is selected from the group consisting of an antigen, an antibody and a nucleic acid probe.