Patent ID: 6037177
Filing Date: 2000-03-14
Classification: C12N

Abstract:
A method for producing a stock of recombinant adeno-associated virus comprising:a) co-transfecting cells permissive for adeno-associated virus replication withi) a recombinant vector engineered to express decreased levels of adeno-associated REP 76/68 protein with respect to levels of expression observed when the host cells express adeno-associated REP 76/68 under regulatorv control of its native promoter;ii) a recombinant vector engineered to express increased levels of adeno-associated REP 40/52 and CAP protein with respect to levels of expression observed when the host cells express adeno-associated REP 40/52 and CAP under regulatory control of their native promoters; andiii) a recombinant adeno-associated virus vector which contains a foreign DNA sequence and which can be incorporated into an infectious AAV virion; andb) collecting the virions produced.