Patent ID: 8415616
Filing Date: 2013-04-09
Classification: C12Q,G01N,H01J

Abstract:
1. A method for determining by mass spectrometry the amount in a sample of two or more analytes selected from the group consisting of 17-hydroxyprogesterone (17-OH progesterone), androstenedione, testosterone, and cortisol, the method comprising: a. subjecting said sample to turbulent flow liquid chromatography to obtain a sample enriched in said two or more analytes subject to determination; b. subjecting said enriched sample to an ionization source under conditions suitable to produce one or more ions detectable by mass spectrometry from each of said two or more analytes; c. determining the amounts of the one or more ions from each of the two or more analytes by tandem mass spectrometry; and d. using the amounts of the one or more ions from each of the two or more analytes to determine the amounts of each of the two or more analytes in the sample; wherein said two or more analytes are not derivatized prior to ionization.