Patent ID: 8394628
Filing Date: 2013-03-12
Classification: A01K,A61K,A61P,C07H,C12N,C12Q

Abstract:
1. A method of mediating RNA interference of an mRNA in a cell in vitro, comprising: (a) introducing into the cell isolated double stranded RNA of from about 21 nucleotides to 23 nucleotides in length, wherein the double stranded RNA is in the form of two separate strands which are not covalently linked and has sequence correspondence to the mRNA, wherein the double stranded RNA is chemically synthesized and wherein one or more nucleotides of the double stranded RNA are a non-naturally occurring nucleotide or deoxyribonucleotide or nonstandard nucleotide, and wherein the double stranded RNA mediates RNA interference by directing cleavage of the mRNA to which it corresponds, wherein cleavage is directed within the region of sequence correspondence with the double stranded RNA, and wherein the mRNA is mammalian cellular mRNA and; (b) maintaining the cell that contains the double stranded RNA such that degradation of the mRNA occurs, thereby mediating RNA interference of mRNA in the cell.