Patent ID: 6015662
Filing Date: 2000-01-18
Classification: G01N

Abstract:
A method for detecting the presence of antibody which may be present in a test sample wherein said method comprises (A) contacting said test sample suspected of containing said antibody with antigen specific for said antibody for a time and under conditions sufficient to allow the formation of antigen/antibody complexes, (B) detecting the presence of said antibody which may be present in said test sample and employing, as a control or calibrator, a reagent which binds to said antigen, wherein the improvement comprises employing, as said control or calibrator, a chimeric antibody comprising murine heavy and light chain variable regions genetically fused to human or a species immunologically cross0reactive with human heavy and light chain constant regions, wherein said chimeric antibody binds to said antigen and is homogeneous with respect to specificity and affinity.