Patent ID: 7727740
Filing Date: 2010-06-01
Classification: C12Q,G01N

Abstract:
1. A method for testing if a chemical agent identified as a chemical agent that decreases the enzymatic activity of an L-2 hydroxy acid oxidase in an in vitro assay decreases the enzymatic activity of an L-2 hydroxy acid oxidase in vivo, comprising administering one or more chemical agents that decrease L-2 hydroxy acid oxidase activity in vitro to a test animal and measuring one or more physiological parameters selected from the group consisting of body weight, body fat, retroperitoneal fat accumulation, serum level of LDL-cholesterol, serum level of HDL-cholesterol, and leptin expression, wherein a decrease in at least one of body weight, body fat, retroperitoneal fat accumulation, serum LDL cholesterol, or leptin expression, in comparison to a control animal not administered the chemical agent, indicates that the chemical agent identified in the in vitro assay decreases the enzymatic activity of an L-2 hydroxy acid oxidase in vivo.