Patent ID: 7910300
Filing Date: 2011-03-22
Classification: C12Q,G01N

Abstract:
1. A method of detecting colorectal tumors, comprising: dividing a test sample of APC alleles isolated from a patient, to form a plurality of aliquots of APC alleles, wherein said aliquots comprise on average between 0 and 20 APC alleles, and wherein the test sample is a body fluid or exudate; amplifying said APC alleles in said plurality of aliquots to form amplified APC alleles; transcribing and translating proteins in vitro using said amplified APC alleles as transcription templates; determining size or composition of said proteins, wherein proteins which differ in size or composition from the protein produced by a wild-type APC allele indicate a mutation in an amplified APC allele which indicates a colorectal tumor in the patient.