Patent ID: 6794158
Filing Date: 2004-09-21
Classification: G01N,Y10S

Abstract:
A fluorescent probe that binds to the active site of soluble epoxide hydrolase, said probe having the formula (I):X-spacer-R1-Yâ€ƒâ€ƒ(I) wherein X is the radical of compound that binds to the active site of soluble epoxide hydrolase, Y is a fluorescent label, â€œspacerâ€  is a direct bond or is a C1-C16 alkylene group, a C2-C16 alkenylene group or a C2-C16 alkynylene group, wherein any of the available â€”CH2â€” groups present in the C1-C16 alkylene group, C2-C16 alkenylene group or C2-C16 alkynylene group can optionally be replaced with O, S(O)p wherein p is 0 to 2, or N(R2), R1 is selected from the group consisting of O, S, â€”N(R2)C(O)â€”, â€”C(O)N(R2)â€”, â€”N(R2)C(S)â€”, â€”C(S)N(R2)â€”, â€”N(R2)C(S)NHâ€”, â€”NHC(S)N(R2), â€”N(R2)C(O)NHâ€”, â€”NHC(O)N(R2), â€”SO2NR2â€”, â€”NR2SO2â€”, â€”CH2N(R2)â€”, â€”N(R2)CH2â€”, â€”CH2Sâ€”, â€”SCH2â€”, â€”C(O)CH2Sâ€”, â€”SC(O)CH2â€”, â€”NHCH2CH2Sâ€”, â€”SCH2CH2NHâ€”, â€”NC(O)Oâ€”, â€”ONC(O)â€”, â€”C(O)Oâ€”, â€”OC(O)â€”, â€”NHâ€”N&boxH;C(R2)â€”, â€”C(R2)&boxH;Nâ€”NHâ€”, â€”NHCH(R2)â€”, â€”CH(R2)NHâ€”, and R2 is selected from H or C1-3alkyl.