Patent ID: 8168381
Filing Date: 2012-05-01
Classification: B01J,C12N

Abstract:
1. A method for synthesizing an encoded molecule comprising the steps of: a) Adding a linker molecule L to one or more reaction wells; b) Adding a molecule fragment to each of said reaction wells; c) Adding an oligonucleotide identifier to each of said reaction wells; d) Subjecting said wells to conditions sufficient to allow said molecule fragments and said oligonucleotide identifiers to become attached to said linker molecule, or conditions sufficient for said molecule fragments to bind to other molecule fragments and sufficient for said oligonucleotide identifiers to bind to other oligonucleotide identifiers, so as to form bi-functional molecules consisting of an encoded molecule and an oligonucleotide; e) Combining the contents of said one or more reaction wells, to produce an admixture of said bi-functional molecules; f) Optionally, distributing the combined product to one or more new reaction wells; g) Optionally, repeating steps b) to f) one or more times; and h) Contacting the resulting bifunctional molecule(s) of step e) or g) with one or more templates each capable of hybridizing to at least one of the oligonucleotide identifiers added in step c); wherein the linker molecule L contains at least one reactive group capable of reacting with a reactive group in the molecule fragment and at least one reactive group capable of reacting with a reactive group in the oligonucleotide; the molecule fragments each contain at least one reactive group capable of reacting with a reactive group in the linker molecule L or a reactive group in another molecule fragment, and the reactive groups of each molecule fragment may be the same or different; the oligonucleotide identifiers each contain at least one reactive group capable of reacting with a reactive group in the linker L or a reactive group in another oligonucleotide identifier, and the reactive groups of each oligonucleotide identifier may be the same or different; the region of the oligonucleotide identifier added to each well in step c) which hybridizes to said template identifies the molecule fragment added to the same well in step b); the steps a) to d) may be performed in any order; the steps b) to d) in step g) may also be performed in any order; the number of wells in steps a) and f) may be the same or different; and the oligonucleotide template optionally is associated with a reactive group.