Patent ID: 7447595
Filing Date: 2008-11-04
Classification: G16B

Abstract:
1. A method for evaluating and identifying putative substrates utilizing a Tat secretory pathway, comprising: a) reading a text file containing one or more amino acid sequences for a predicted protein; b) comparing residues 2 through 35 of the N′ terminal residues of the predicted protein against a Tat signal sequence motif, wherein the sequence motif is identified as R X c) if the Tat sequence motif is identified, verifying an uncharged stretch of at least 13 amino acid residues in the 22 residues following twin arginines of the predicted protein; d) calculating the hydrophobicity sum of the first 13 residues of the uncharged region; verifying the hydrophobicity sum is a value less than 8.0; and if the motif is identified and the criteria c) and d) are met, e) identifying the predicted protein as a putative Tat substrate; and f) outputting amino acid sequence of the predicted protein to user.