Patent ID: 6692596
Filing Date: 2004-02-17
Classification: B01L

Abstract:
Method of manufacturing a micro-titer test plate comprising a plurality of sample containers connected to each other of which each sample container has one or more side walls enclosing the interior of said sample container, a bottom wall with an outlet opening and an opposite upper end that is open and defines an inlet opening, said method comprising the steps of:(a) providing a first aid second part said first part comprising a plurality of wells connected to each other and each having an upper end that is open and that will define the inlet opening of said sample containers and an opposite bottom opening and said second part comprising a plurality of spouts connected to each other, said spouts conforming in arrangement and number to said wells of said first part and each spout enclosing at a first end an opening that will define the outlet opening of said sample containers, and each spout optionally having an upper opening at said first end for receiving a filter means; (b) placing a filter sheet that extents across each of said wells of said first part on one side of said first part or placing a filter sheet that extends across each of said spouts of said second part on the side of said second part having said first ends; (c) separating from said filter sheet filter means that conform in shape, size, arrangement and number to either the bottom opening of the wells of said first part or when present, to said optional upper openings provided at the first end of the spouts; (d) placing said filter means in each of the bottom openings of the wells or when present in each of said upper openings provided at the first end of the spouts; (e) removing the remainder of said filter sheet from which the filter means have been separated; (f) bringing said first part and said second part in contact with each other such that the bottom opening of said wells face the first end of said spouts; and (g) bonding said first part and said second part to each other by permanently and irreversibly bonding each of the wells of said first part to each of the spouts of said second part thereby forming a plurality of sample containers connected to each other that are scaled with respect to each other.