Patent ID: 6033918
Filing Date: 2000-03-07
Classification: G01N,Y10S

Abstract:
In an immunoassay for deoxypyridinoline (DPD) as analyte in a fluid test medium in which a labeled antibody specific to the DPD analyte is combined with the fluid test medium, which fluid test medium is then contacted with a solid support of nitrocellulose upon which the DPD analyte or analyte derivative is immobilized so that labeled antibody which has not reacted with the DPD analyte in the test medium will react and be bound by the immobilized DPD analyte or derivative thereof, the improvement which comprises immobilizing the DPD analyte or analyte derivative on the solid support using a conjugate prepared by reacting the DPD analyte or derivative thereof with polyethylene glycol having a molecular weight of greater than about 6,000 up to 50,000 functionalized on one or both ends with a group reactive with the DPD analyte or derivative thereof which reactive group is activated carboxy, amino, epoxy, halo, sulfhydryl, isocyanate maleimide or formyl and, when functionalized on only one end with a reactive group, the polyethylene glycol is functionalized on the other end with an unreactive capping group, wherein the reactive group acts to bind the DPD analyte or analyte derivative.