Patent ID: 6255099
Filing Date: 2001-07-03
Classification: C12N

Abstract:
A dual-buffer bacterial growth medium comprising:(a) an effective amount of a first buffer having a pKa between about 6.6 and about 7.5;(b) an efective amount of a second buffer having a pKa between about 7.5 and about 8.0;(c) an effective amount of free RNA;(d) an effective amount of an RNase enzyme; and(e) an effective amount of bacterial nutrients; wherein:(f) the pH of said medium is about 7.6 to about 7.7; and wherein:(g) the medium supports a signficantly higher density growth of bacterial cell cultures over time than either a single-buffer growth medium or a dual-buffer growth medium outside the pH range of about 7.6 to about 7.7.