Patent ID: 6610514
Filing Date: 2003-08-26
Classification: C07K,C12N,C12Y

Abstract:
A method for transforming Candida utilis, comprising the steps of transforming cells of C. utilis with a vector that comprises a marker gene for selecting transformants, a shortened promoter sequence that is operably linked to the marker gene, and a homologous DNA sequence that is homologous to chromosomal DNA, other than an rDNA sequence, of Candida utilis, and optionally a heterologous gene or a gene isolated from C. utilis, wherein the vector is linearized by cleaving within or at both ends of the homologous DNA sequence with a restriction enzyme, wherein the heterologous gene or gene isolated from Candida utilis can be integrated into the chromosomal DNA of Candida utilis by homologous recombination, and wherein the shortened promoter is a promoter truncated at its 5â€² terminus to decrease, relative to pCLRE11, the transformation frequency of the vector in host cells of Candida utilis and to increase, relative to pCLRE11, copy number of the vector in host cells of Candida utilis and then selecting the resulting transformants which show drug resistance.