Patent ID: 8773520
Filing Date: 2014-07-08
Classification: G02B

Abstract:
1. A method for obtaining an observation image of a sample using an observation system, the method comprising steps of: (a) preparing the observation system; wherein the observation system comprises an observation unit and a computer; the observation unit comprises: the computer comprises a memory; (b) loading the container with the sample accommodated therein; (c1) moving the loading table while the illumination device illuminates the sample, so as to search the sample with the observation unit; wherein when a shutter speed of the observation unit is set to a lower limit value of a range that can be set and a luminance of the observation image fails to fall within a predetermined range of values, the shutter speed of the observation unit is not changed and the illumination device adjusts an illuminance of the illumination device, (c2) recording the coordinates of the sample which has been searched in the step (c1); (d1) moving the loading table on the basis of the coordinate of the sample read from the memory; (d2) illuminating the illumination device by the illuminance which is greater than the illuminance configured in the illumination device in the step (c1); (d3) conducting auto focus control of the observation device; (d4) adjusting the shutter speed of the observation device without changing the illuminance in the illuminating device, when the luminance of the observation device fails to fall within the predetermined range of values; (d5) acquiring the observation image of the sample using the observation device; and (e) repeating the steps (d1)-(d5), after a predetermined period lapses.