Patent ID: 6480791
Filing Date: 2002-11-12
Classification: C12N,C12Q

Abstract:
A parallel method for polynucleotide sequencing, comprising:a) preparing a library comprising a collection of two or more sample-tagged polynucleotide clones; b) carrying out a nucleic acid sequencing reaction on the library wherein a first sequencing primer binding site or no sequencing primer binding site is used to generate a plurality of tagged reaction products from the sample-tagged clones in the collection; c) separating the reaction products according to size; d) collecting fractions of the separated reaction products; e) amplifying the products collected in step (d) to generate tagged amplicons; f) hybridizing the tagged amplicons to an array comprising tag complements; and g) determining a plurality of polynucleotide sequences of the sample-tagged clones by detecting the hybridizations to deconvolute a plurality of sequence ladders for the sample-tagged clones in the collection.