Patent ID: 6294670
Filing Date: 2001-09-25
Classification: C07F

Abstract:
A probe for detecting a highly ordered structural site of a nucleic acid of a gene by specifically binding with the structural site to generate an electrochemical response, said probe comprising a cyclic ligand containing a ferrocenyl group bonded to a DNA threading intercalating moiety selected from the group consisting of 1,4,5,8-tetrasubstituted naphthalene and 1,5-disubstituted anthraquinone, wherein the cyclic ligand further comprises two linker moieties, wherein said linker moiety comprises an amine selected from the group consisting of 1,4-bis(3-aminopropyl)piperazine, 1,1 '-bis(3-aminopropyl)methylamine, 1,1'-bis(2-aminoethyl)amine, 1,1'-bis(3-aminopropyl)amine, spermine and spermidine, each linker moiety, before it is incorporated into the cyclic ligand, has two terminal amino groups, each linker moiety is connected with the DNA threading intercalating moiety through one of said terminal amino groups, and each linker moiety is connected with the ferrocenyl group through the other said terminal amino group.