Patent ID: 6350578
Filing Date: 2002-02-26
Classification: C12Q,Y10T

Abstract:
A method for quantitating dsDNA in a sample, comprising the steps of:(a) preparing a buffered test solution comprising a fluorescence attenuating material, a known amount of dsDNA, and a dye in an amount sufficient to combine with all of the dsDNA in the test solution to provide a highly fluorescent dye-dsDNA complex; (b) measuring the fluorescence intensity of the test solution; (c) preparing a diluted test solution from the test solution of step (a) and measuring fluorescence intensity of the diluted test solution; (d) repeating step c) until a sufficiently diluted test solution is prepared having a measured fluorescence intensity that is not attenuated upon further dilution; (e) measuring the absorbance of the sufficiently diluted test solution of step d); (f) preparing a sample solution comprising dsDNA extracted from the sample and having a measured absorbance equal to that of the sufficiently diluted test solution of step e); (g) adding the dye to the sample solution in an amount sufficient to combine with all of the dsDNA in the sample solution to provide the dye-dsDNA complex; and (h) measuring the fluorescence intensity of the dye containing sample solution of step g).