Patent ID: 6521427
Filing Date: 2003-02-18
Classification: B01J,C12N,C40B

Abstract:
A method of synthesizing a double-stranded polynucleotide, comprising:(a) annealing a 5â€² terminal oligonucleotide with a 3â€² terminal oligonucleotide of a double-stranded polynucleotide to produce a first annealed product and directly adding a next most terminal oligonucleotide of said double-stranded polynucleotide to said first annealed product under conditions sufficient for annealing to produce a second annealed product, said next most terminal oligonucleotide having a length of at least about 25 bases, and (b) repeating step (a) one or more times to sequentially produce a double-stranded polynucleotide.