Patent ID: 8962245
Filing Date: 2015-02-24
Classification: C07H,C12N,C12Q

Abstract:
1. A method for producing a circular DNA, the method comprising the following steps: 1) providing a DNA molecule; 2) a step of covalently binding an adapter (A) to one end of the DNA molecule, and covalently binding to the other end of the DNA molecule an adapter (B) comprising an adapter (b) and the adapter (A), wherein: 3) a first cleavage step of cleaving the DNA molecule obtained in step 2) at the cleavage site of each of the adapters (A); 4) a first circularization step of binding both ends of the DNA molecule obtained in step 3) to circularize the DNA molecule; 5) a step of eliminating uncircularized linear DNA molecules obtained in step 4); 6) a second cleavage step of cleaving the circular DNA molecules from step 5) at the cleavage site of the adapter (b); and 7) a second circularization step of binding both ends of the DNA molecule obtained in step 6) to circularize the DNA molecule and obtain a circular DNA.