Patent ID: 7939326
Filing Date: 2011-05-10
Classification: C07K

Abstract:
1. A method of producing recombinant lentivirus particles in modified cells in a culture vessel, comprising: introducing into the modified cells, which are modified to comprise a stably integrated recombinant nucleic acid selected from the group consisting of: i) a nucleic acid encoding an integrin β ii) a nucleic acid encoding an integrin α iii) any combination of (i) and (ii), and which have at least three fold increased adherence as compared to unmodified cells, the following: I) a first vector comprising a lentivirus nucleic acid sequence encoding at least one lentivirus structural protein, wherein said vector (1) comprises at least one defect in at least one gene encoding a lentivirus structural protein, and (2) comprises a defective packaging signal; II) a second vector comprising a lentivirus nucleic acid sequence comprising cis-acting sequence elements required for reverse transcription of the vector genome wherein said vector comprises a competent packaging signal; and III) a third vector comprising a nucleic acid sequence of a virus, wherein said third vector (1) expresses a viral envelope protein, and (2) comprises a defective packaging signal, under conditions whereby recombinant lentivirus particles are produced.