Patent ID: 8679844
Filing Date: 2014-03-25
Classification: C12N

Abstract:
1. An expression cassette for expression of a nucleic acid molecule product in the reproductive tissue of a plant, comprising: a) a promoter, a first exon; a first intron, and a second exon of a plant MADS gene, wherein said promoter, first exon, intron, and second exon are the 5′-regulatory sequence of said expression cassette; wherein said 5′-regulatory sequence has been engineered 1) to include a translational initiation codon at approximately the 3′ end of said 5′-regulatory sequence, 2) to eliminate restriction endonuclease sites that hinder manipulation by recombinant DNA methods, and 3) to modify any additional translation initiation codons upstream of said translation initiation codon to be inoperable so that the MADS gene's protein coding sequence is silent, while preserving the length of the 5′-regulatory sequence in the expression cassette; b) a 3′-regulatory sequence of a plant MADS gene; and c) a nucleic acid molecule operably linked to said 5′-regulatory sequence and said 3′-regulatory sequence, wherein the product of the expression of said nucleic acid molecule is targeted to the reproductive tissue of a plant.