Patent ID: 6013462
Filing Date: 2000-01-11
Classification: A61K,C07K,C12N,G01N,Y10S

Abstract:
A method to purify or characterize a human glutathione-S-transferase (GST) enzyme from a sample, which method comprises contacting said sample with a solid support to which is coupled a compound of the formula ##STR6## or the alkyl (1-10C), alkenyl (1-10C), or arylalkyl (7-12C) esters or amides or saltswherein Z is selected from the group consisting of S, O and C;wherein n is 1 or 2;wherein when Z is S and n is 1, X is a mono- or disubstituted or unsubstituted hydrocarbyl (1-20C) moiety optionally containing 1 or 2 nonadjacent heteroatoms (O, S or N), and wherein said substitution is selected from the group consisting of halo, --NO, --NO.sub.2, --NR.sub.2, OR, and SR, wherein R is H or lower alkyl (1-4C); when Z is S and n is 2, one X is as above defined and the other X is lower alkyl (1-4C);Y is selected from the group consisting of ##STR7## wherein m is 1 or 2; and AA.sub.C is valine, alanine, .beta.-alanine, 4-aminobutyric acid, aspartic, phenylglycine, histidine, tryptophan, tyrosine, phenylalanine linked through a peptide bond to the remainder of the compound of formula 1;under conditions wherein said human GST is adsorbed to said support,separating the solid support from the sample, andeluting said human GST from the solid support by providing an eluting solution.