Patent ID: 8697851
Filing Date: 2014-04-15
Classification: A01K,C12N,C12Y

Abstract:
1. A targeting construct, comprising from 5′ to 3′: a. a mouse targeting arm; b. a first recombinase recognition site; c. a nucleic acid sequence encoding a selectable marker operably linked to a first promoter; d. a second promoter operably linked to a recombinase gene, wherein the recombinase gene encodes a recombinase that recognizes the first and a second recombinase recognition sites; e. a 3′-UTR comprising an miRNA recognition site; f. a second recombinase recognition site, wherein the first recombinase recognition site and the second recombinase recognition site are oriented relative to one another to direct an excision; and, g. a mouse targeting arm, wherein the miRNA recognition site is recognized by an miRNA that is active in an undifferentiated cell but is not active in a differentiated cell so that the nucleotide sequence flanked by the first and the second recombination sites is excised from a genome of the differentiated cell wherein the recombinase gene is transcribed and expressed.