Patent ID: 9012607
Filing Date: 2015-04-21
Classification: A61K,A61P,C07K,G01N

Abstract:
1. A mutated humanized 12G4 monoclonal antibody, comprising or consisting of: a) a light chain comprising or consisting of: b) a heavy chain comprising or consisting of: wherein said mutated humanized 12G4 monoclonal antibody comprises at least one mutation selected from the group consisting of: a mutation located in one of the CDR regions of the VL that is a substitution of at least one amino acid selected from the group consisting of S179P, one of E184K, E184G, or E184D, and S182F, a mutation located in one of the FR regions of VL that is a substitution of at least one amino acid selected from the group consisting of: I177T, I132T, A1431, 1150A, S158P, L175Q, Y178H, V187A, 51921, G197D, and F212S, and a mutation located in the heavy chain that is a substitution of at least one amino acid selected from the group consisting of: Q1E, Q3E or Q3R, Q6E, A9T, V11A, K12R, K13R, K19E, V20A, one of A24G, A24V or A24T, Q39E, A40V, S31G, L45P, D56N, A76T, A79T, R87G, T58A, Q62R, V67M, I70N, T74A, S77P, A79T, S88P, E89D, F102S, A1031, L110P, and 51141, and said mutated humanized 12 G4 monoclonal antibody has a K a) a light chain consisting of: b) a heavy chain consisting of: for said receptor below 10