Patent ID: 9193977
Filing Date: 2015-11-24
Classification: A01K,A61K,C07K,C12N,C12Y,G01N

Abstract:
1. A method of making a mouse, the method comprising: (a) replacing exons 2, 3 and 4 of a mouse SIRPα gene at an endogenous mouse SIRPα locus in a mouse ES cell with exons 2, 3 and 4 of a human SIRPα gene to form a humanized SIRPα gene, wherein said humanized SIRPα gene is operably linked to a mouse SIRPα promoter at said endogenous mouse SIRPα locus and encodes a humanized SIRPα protein comprising an extracellular portion of the human SIRPα protein encoded by said human SIRPα gene and an intracellular portion of the mouse SIRPα protein encoded by said mouse SIRPα gene, thereby obtaining a modified mouse ES cell comprising said humanized SIRPα gene; (b) creating a mouse using the modified ES cell of (a).