Patent ID: 6100027
Filing Date: 2000-08-08
Classification: C12Q

Abstract:
A probe mix comprising:a first hybridization assay probe comprising a first oligonuoleotide containing 10 to 100 nucleotide bases which hybridizes to at least 10 contiguous nucleotide bases of a first target nucleic acid region present in target nucleic acid from Neisseria meningitidis subtypes A, C, and L and which does not detectably hybridize to nucleic acid from Neisseria gonorrhoeae under stringent hybridization conditions, wherein said first oligonucleotide contains a region of at least 10 contiguous nucleotide bases which is at least 80% perfectly complementary to said at least 10 contiguous nucleotide bases of said first target region, said first target region being selected from the group consisting of:SEQ ID NO. 11;SEQ ID NO. 27;SEQ ID NO. 15; andSEQ ID NO. 25; anda second hybridization assay probe comprising a second oligonucleotide containing 10 to 100 nucleotide bases which hybridizes to at least 10 contiguous nucleotide bases of a second target nucleic acid region present in a target nucleic acid from Neisseria meningitidis subtype L and which does not detectably hybridize to nucleic acid from Neisseria gonorrhoeae under said hybridization conditions, wherein said second oligonucleotide contains a region of at least 10 contiguous nucleotide bases which is at least 80% perfectly complementary to said at least 10 contiguous nucleotide bases of said second target region, said second target region being selected from the group consisting of:SEQ ID NO. 12;SEQ ID NO. 26;SEQ ID NO. 16; andSEQ ID NO. 28.