Patent ID: 7604998
Filing Date: 2009-10-20
Classification: C12Q,G01N,Y10T

Abstract:
1. A method of detecting the presence or absence of a constituent oligonucleotide in an oligonucleotide mixture, the method comprising: a) obtaining a measurement of a mass spectrum of the oligonucleotide mixture; b) comparing the measurement of the mass spectrum having a plurality of measured peaks with a calculated mass spectrum having a plurality of calculated peaks, said calculated mass spectrum derived from Formula 1: wherein MW new is a calculated molecular weight of a calculated peak, MW 1 is a first molecular weight of a first subset of one or more oligonucleotides in the oligonucleotide mixture, MW 2 is a second molecular weight of a second subset of one or more oligonucleotides in the oligonucleotide mixture, MW n represents one or more additional molecular weights of one or more additional n subsets in the oligonucleotide mixture, Intensity 1 is a first peak intensity of the first subset of one or more oligonucleotides in the oligonucleotide mixture, Intensity 2 is a second peak intensity of the second subset of one or more oligonucleotides in the oligonucleotide mixture and Intensity n represents one or more additional peak intensities of the one or more additional n subsets in the oligonucleotide mixture; and c) detecting the presence or absence of the constituent oligonucleotide by correlating one or more of the measured peaks with one or more of the calculated peaks.