Patent ID: 8969014
Filing Date: 2015-03-03
Classification: G01N

Abstract:
1. A method of determining whether a non-purified sample contains a target protein bound to a ligand of interest comprising the steps of: a) exposing the non-purified sample to a temperature which is capable of causing or enhancing precipitation of the unbound target protein to a greater extent than it is capable of causing or enhancing precipitation of the target protein bound to the ligand wherein the target protein in said non-purified sample is comprised within or on a cell; b) subjecting said sample to conditions capable of causing cell lysis; c) separating soluble from insoluble protein in the product of step b); and d) analyzing either or both the soluble and insoluble protein fractions of step c) for the presence of the target protein, wherein the target protein is detected by affinity binding a detection moiety or by mass spectrometry.