Patent ID: 6576758
Filing Date: 2003-06-10
Classification: A61K,A61P,C12N

Abstract:
A nucleic acid construct for the regulated expression of a transgene in a host cell comprising in a 5â€² to 3â€² end direction reading frame, the following components:(i) a first promoter or enhancer sequence (a), which activates the transcription of said transgene or a first promoter or enhancer sequence (aâ€²) which contains a mutation which inhibits the function of the first promoter (aâ€²); (ii) a transgene (bâ€²) encoding a pharmacologically active compound or a transgene (b) containing (1) a nuclear retention signal (NRS) which is linked, at its 5â€² end to the 3â€² end of the transgene, wherein the transcription product of the NRS provides a structure for binding a nuclear export factor (NEF), and (2) a mutation which inhibits the transcription and/or the translation of said transgene or inhibits the function of the pharmacologically active compound encoded by said transgene; (iii) a second promoter or enhancer sequence (c), which activates the basal transcription of the component (d) or which contains a mutation which inhibits the function of the second promoter; and (iv) a gene (d) encoding a tRNA or a regulatory protein for abolishing the mutation in at least one of the promoters (a) and (c) or in the transgene (b).