Patent ID: 7238505
Filing Date: 2007-07-03
Classification: C12N

Abstract:
1. An immobilized DNA polymerase comprising a DNA polymerase having an active site and an immobilization site, the immobilized DNA polymerase further comprising a linker, a matrix molecule, and a substrate material, wherein the DNA polymerase is bonded by a covalent bond with to the linker; the linker is bonded by a chemical bond to the substrate material; the immobilization site of the DNA polymerase is distant from the active site; the matrix molecule comprises a non-reactive terminal group and is connected to the substrate material together with the linker, and wherein the average number of covalent bonds formed between the immobilized DNA polymerase and the linker is such that average activity of the immobilized DNA polymerase is more than about 10% of the activity of solution phase DNA polymerase.