Patent ID: 8592183
Filing Date: 2013-11-26
Classification: C12Q

Abstract:
1. A method for sequencing a target polynucleotide, comprising: a. immobilizing the target polynucleotide on a support of an apparatus for sequencing a polynucleotide, comprising: b. contacting the target polynucleotide with a polymerase enzyme and a nucleotide selected from the group consisting of A, T(U), G, and C under conditions suitable for a polymerase reaction to proceed, wherein the nucleotide comprises a removable blocking group; c. observing the interaction of the polymerase enzyme and the nucleotide with the target polynucleotide; d. determining whether the polymerase has incorporated the nucleotide onto the primer sequence; and e. repeating steps (b)-(d) one or more times, thereby identifying the sequence of the target polynucleotide.