Patent ID: 6372431
Filing Date: 2002-04-16
Classification: A61P,C12Q

Abstract:
A method for detecting or diagnosing the effect of a toxic compound or molecule associated with increased or decreased levels of nucleic acid molecules in a mammalian subject comprising:a) treating a mammalian subject with a toxic compound or molecule; b) obtaining a sample containing nucleic acids from the mammalian subject treated with the toxic compound or molecule; c) contacting the sample with a microarray comprising a plurality of nucleic acid molecules consisting of SEQ ID NOs:1-47 or fragments thereof under conditions for the formation of one or more hybridization complexes, wherein the fragments are polynucleotides consisting of the first sixty consecutive nucleotides of a polynculeotide selected from SEQ ID NOs:1-47; and d) detecting the hybridization complexes, wherein the presence, absence or change in amount of the hybridization complex, as compared with the hybridization complexes formed from nucleic acid molecules from an untreated mammalian subject, is indicative of a metabolic response to the toxic compound or molecule.