Patent ID: 7347922
Filing Date: 2008-03-25
Classification: G01N

Abstract:
1. A method for generating a two-dimensional protein expression profile for determining protein: protein interactions in a sample said method comprising the steps of: separating a non-denatured protein sample in a first dimension by a non-denaturing cationic (CAT) gel electrophoresis that retains protein: protein interactions during the non-denaturing electrophoretic separation; denaturing the separated proteins in the non-denatured gel to produce denatured proteins; and separating the denatured proteins by a denaturing gel electrophoresis in a second dimension substantially orthogonal to the first dimension using denaturing CAT electrophoresis following the non-denaturing CAT electrophoresis used in the first dimension to produce a two-dimensional protein expression profile having a plurality of protein bands positioned substantially along a diagonal axis relative to the first dimension and the second dimension, each band representing one or more unbound proteins, with any proteins forming multi-protein aggregates with the unbound proteins appearing as one or more bands in positions offset from the diagonally positioned bands, with the offset positions defining a grouping corresponding to the molecular weight of each diagonally positioned protein band.