Patent ID: 8034554
Filing Date: 2011-10-11
Classification: C12Q

Abstract:
1. A method of detecting the presence of a target nucleic acid in a sample comprising the steps of: a) providing a biological sample containing a target nucleic acid, b) mixing said sample with a nucleic acid hairpin capture probe, c) specifically hybridizing said target-complementary sequence to a target sequence in said target nucleic acid, d) binding said capture sequence to a complementary immobilized probe sequence, thereby forming a capture hybrid made up of said target nucleic acid, said capture probe, and said immobilized probe attached to said capture support, e) separating said capture hybrid attached to said capture support from sample components, f) releasing said target nucleic acid from said capture hybrid, g) hybridizing a detection probe to said target nucleic acid to form a detection hybrid, and h) detecting a signal produced in a homogeneous reaction from said detection hybrid to indicate the presence of said target nucleic acid.