Patent ID: 6406700
Filing Date: 2002-06-18
Classification: C07K,C12N,C40B,Y10S

Abstract:
A method of inhibiting the growth or proliferation of a type of cancer in an individual in whom such inhibition is desired comprising administering to the individual a composition comprising an immunogenic amount of a population of complexes of heat shock protein noncovalently bound to peptides, said complexes being produced by:(a) making cDNA molecules from RNA molecules of a cancer cell or a preneoplastic cell of said type of cancer; (b) ligating the cDNA molecules into an expression construct such that substantially each cDNA molecule is operably associated with at least one regulatory region that controls expression of the cDNA molecule, to form a library of expression constructs; (c) introducing the library into one or more host cells; (d) culturing the host cells containing the cDNA molecules under conditions such that proteins encoded by the cDNA molecules are expressed by the host cells; and (e) recovering from the host cells complexes of heat shock protein noncovalently associated with one or more peptides.