Patent ID: 9045731
Filing Date: 2015-06-02
Classification: A61K,A61P,C12N,C12Y

Abstract:
1. An in vitro method for increasing the function of sarcoplasmic reticulum (SR) in or improving Ca 2+ handling, of a stem cell-differentiated human cardiomyocyte comprising functionally mature sarcoplasmic reticulum (SR), the method comprising enhancing the expression of a calcium handling protein selected from calsequestrin, junctin, triadin or phospholamban in the stem cell-differentiated human cardiomyocyte, wherein the cardiomyocyte is differentiated from a stem cell in vitro and is caffeine-responsive, wherein the enhancing the expression of the calcium handling protein comprises transfecting the cardiomyocyte with a nucleotide encoding the calcium handling protein.