Patent ID: 9080256
Filing Date: 2015-07-14
Classification: C07K,C12N,C40B

Abstract:
1. A method of producing a library of soluble polypeptides linked to polynucleotides encoding the polypeptides, comprising: obtaining a plurality of polynucleotides comprising a plurality of codons, which encode polypeptides, wherein a proportion of hydrophilic or surface compatible amino acid residues encoded by said codons satisfies the following formula: wherein N=the number of amino acids in each polypeptide, and c=fraction of amino acids that are hydrophilic or surface compatible; and translating the plurality of synthesized polynucleotides to generate the encoded polypeptides in a manner such that each polypeptide is linked to the polynucleotide that encodes it, thereby producing a library of soluble polypeptides linked to polynucleotides encoding the polypeptides.