Patent ID: 9169493
Filing Date: 2015-10-27
Classification: C12N

Abstract:
1. A method for propagating a fully-deleted adenovirus-based gene transfer vector comprising: (a) providing an Adenovirus packaging cell line; (b) transfecting, into the cell line, a fully-deleted Adenoviral vector construct; and (c) transfecting, into the cell line, a replication defective packaging construct having a subset of Adenoviral late genes while being absent of at least one inverted terminal repeat and a packaging signal, wherein the fully-deleted Adenoviral vector construct and the packaging construct can transfect the Adenovirus packaging cell line resulting in the encapsidation of a fully-deleted adenoviral-based gene transfer vector independent of helper Adenovirus, wherein the encapsidated fully-deleted adenovirus-based gene transfer vector includes both adenoviral inverted terminal repeats, the packaging signal, and at least one DNA insert which comprises a gene sequence encoding a protein of interest, and wherein the encapsidated fully-deleted adenovirus-based gene transfer vector is absent of adenoviral structural genes.