Patent ID: 6344341
Filing Date: 2002-02-05
Classification: C07K,C12N

Abstract:
A process for increasing the production of a secreted foreign or endogenous protein in a yeast cell, which process comprises:(a) obtaining a vector comprising an isolated DNA molecule encoding said protein; (b1) transforming the vector obtained into a yeast host comprising a DNA molecule encoding Seb1 protein which has an amino acid sequence depicted in SEQ ID NO:2, and overexpressing SEB1 gene, to obtain recombinant yeast host cells; or, (b2) transforming the vector obtained into a yeast host and retransforming this transformant with a yeast expression vector comprising a DNA molecule encoding Seb1 protein which has an amino acid sequence depicted in SEQ ID NO:2, to obtain recombinant yeast host cells overexpressing the SEB1 gene; (c) screening for cells with enhanced production of secreted foreign or endogenous protein as compared with cells not overexpressing the SEB1 gene, and (d) cultivating said recombinant yeast host cells under conditions permitting expression of said secreted foreign or endogenous protein.