Patent ID: 6043036
Filing Date: 2000-03-28
Classification: C12Q,G16B

Abstract:
A method for obtaining the primary sequence of a nucleic acid template comprising:a) preparing, complementary to said nucleic acid template, a set of mixtures of oligonucleotide fragments containing a label, each fragment of a mixture terminated with any single nucleotide terminator selected from a unique two-nucleotide or a unique three-nucleotide terminator combination;b) separating the labeled fragments in each mixture of said set of mixtures of oligonucleotide fragments according to length by an electrophoretic means;c) detecting the oligonucleotide fragments according to their label in each separated mixture of said set of mixtures;d) identifying the relative spacing of said oligonucleotide fragments in each separated mixture of the set of mixtures independent of the separated oligonucleotide fragments in the other mixtures in the set of mixtures;e) producing a set of sequence patterns, each sequence pattern in the set of sequence patterns being based on the relative spacing of said oligonucleotide fragments in each single separated mixture independent of the other mixtures in the set of mixtures;f) applying an alignment algorithm to said set of sequence patterns.