Patent ID: 8932828
Filing Date: 2015-01-13
Classification: A61K,A61P,C07K

Abstract:
1. A method for preparing a biologically active recombinant granulocyte colony-stimulating factor (G-CSF) protein lacking a non-natural N-terminal methionine residue, the method comprising the steps of: (a) obtaining an (b) growing the (c) lysing the cells; (d) separating the soluble proteins from the insoluble proteins, wherein the insoluble proteins comprise the G-CSF; (e) denaturing and reducing the insoluble G-CSF protein; and (f) refolding the G-CSF protein into a biologically active form, wherein the refolded G-CSF protein lacks a non-natural methionine residue and the STII signal sequence.