Patent ID: 6399312
Filing Date: 2002-06-04
Classification: A61P,C07K

Abstract:
A method of identifying a composition which inhibits activation of the myostatin promoter comprising the steps of:a) constructing a vector comprising (SEQ ID NO: 1) and a nucleic acid sequence encoding a reporter molecule, said nucleic acid sequence encoding said reporter molecule is operably linked to SEQ ID NO: 1 wherein said nucleic acid sequence encoding said sequence represented by FIG. 2; b) introducing said vector into a host cell for a time and under conditions suitable for expression of myostatin; c) exposing said host cell to a composition which may inhibit activation of the myostatin promoter and a substrate specific for said reporter molecule; and d) measuring the signal generated by reaction of said reporter molecule and said substrate in comparison to that produced by a control host cell, a smaller signal by said host cell of (c) indicating that said composition will inhibit activation of said myostatin promoter.