Patent ID: 8273688
Filing Date: 2012-09-25
Classification: C07K

Abstract:
1. A collection comprising diverse, isolated human or humanized antibody H-CDR3 regions, wherein the H-CDR3 regions comprise a first, second and third range of amino acid lengths, wherein the first range consists of 4 to 7 amino acids in length, wherein the amino acids of the first range of lengths comprise diversity according to a first diversity factor, wherein the second range consists of 8 to 14 amino acids in length, wherein the amino acids of the second range of lengths comprise diversity according to a second diversity factor, and wherein the third range consists of 15 to 23 amino acids in length, wherein the amino acids of the third range of lengths comprise diversity according to a third diversity factor, wherein the diversity factors comprise a frequency rate at which amino acids appear at each position in the H-CDR3 region, and wherein the first, second and third diversity factors are different, wherein the H-CDR3 regions consisting of a length of 4 amino acids comprise at position 1 Aspartic acid and Glycine have a frequency rate of about 25, Valine, Glutamic acid, Alanine, Leucine, Arginine and Serine have a frequency rate of about 5%, all other amino acids except Cysteine have a frequency rate of about 1.1%; at position 2 Arginine, Glycine, Serine and Tyrosine have a frequency rate of about 10%, Alanine, Aspartic acid, Histidine, Isoleucine and Threonine have a frequency rate of about 5% and all other amino acids except Cysteine have a frequency rate of about 1.8%; at position 3 Glycine has a frequency rate of about 25%, Tyrosine, Serine, Aspartic Acid and Arginine have a frequency rate of about 10% and all other amino acids except Cysteine have a frequency rate of about 1.8%; and at position 4 Glycine has a frequency rate of about 25%, Tyrosine, Serine, and Alanine have a frequency rate of about 10% and all other amino acids except Cysteine have a frequency rate of about 2.4%.