Patent ID: 8309342
Filing Date: 2012-11-13
Classification: C12M,C12N

Abstract:
1. An apparatus for isolating non-lipid filled cells from adipose tissue comprising: a chamber adapted to receive and dissociate the adipose tissue into a dissociated mixture including cells and cell clusters; a lipid separating unit in fluid communication with the chamber and adapted for phase separation of the dissociated mixture into a lipid layer and an aqueous non-lipid filled cellular layer without centrifugation, said lipid separating unit having at least one dispersing port equipped with a plurality of dispersing pores through which the dissociated cell mixture enters the lipid separating unit, wherein said dispersing port is disposed proximally to a bottom inner surface of the lipid separating unit; and at least one dispersing filter disposed in the fluid communication between the chamber and the lipid separating unit.