Patent ID: 6555373
Filing Date: 2003-04-29
Classification: A61K,C07K,C12N,C12P

Abstract:
An isolated recombinant human cell which comprises a heterologous promoter sequence in operable linkage with an endogenous gene which encodes a protein, wherein 106 of said cell produces at least 200 ng of said protein per 24 hours, wherein said endogenous gene is not amplified, wherein the isolated human recombinant cell is produced by endogenous gene activation of an endogenous gene in a human cell that is polysomic for a chromosome containing the endogenous gene, wherein said human cell doubles in suspension culture at least 5 times within 14 days and doubles at least 5 times in a serum-free medium within 14 days and wherein the human cell does not express the endogenous gene prior to endogenous gene activation.