Patent ID: 6399057
Filing Date: 2002-06-04
Classification: A61K,A61Q,C12N

Abstract:
A method for the cosmetic reproduction of hair, which method comprises:(a) removing hair in the anagen phase from one or more donor regions by plucking so that a bulb is still attached to the hair removed, wherein the removing of hair in the anagen phase is done by plucking the hair out of the donor region(s), and selecting hairs in the anagen phase; (b) culturing keratinocytes from the hair removed under conditions whereby keratinocytes multiply, wherein the keratinocytes are cultured in a culture medium supplemented with (i) at least one human mast cell line and/or autologous cultured CD34+ cells, or (ii) one or more extracts of a human mast cell line(s) and/or of the autologous CD34+ cells, and/or (iii) growth-stimulating agents; and (c) introducing the cultured keratinocytes into the pores of receptor regions.