Patent ID: 6335162
Filing Date: 2002-01-01
Classification: C12Q,Y10S

Abstract:
A method of detecting deoxyribonucleic acid in a reaction containing pyrophosphate, adenosine 5â€²-diphosphate, or a combination thereof, the method comprising:depolymerizing a deoxyribonucleic acid at a terminal nucleotide by enzymatically cleaving the terminal internucleotide phosphodiester bond and reforming same with a pyrophosphate molecule to form a free deoxyribonucleoside triphosphate molecule according to the reaction: dNAn+PPiâ†’dNAnâˆ’1+dNTP; enzymatically transferring terminal 5â€² phosphate groups from the deoxyribonucleoside triphosphate molecules to adenosine 5â€²-diphosphate molecules to form adenosine 5â€²-triphosphate according to the following general reaction: dNTP*+ADPâ†’dNDP+ATP*, wherein P* is the terminal 5â€² phosphate so transferred; and detecting the adenosine 5â€²-triphosphate formed thereby.