Patent ID: 6262026
Filing Date: 2001-07-17
Classification: A61K,A61P,C07K,C12N

Abstract:
A method of introducing a selected substance into a mammalian cell, comprising contacting the cell with a chimeric protein comprising a functional domain and a carrier domain, whereinthe functional domain comprises a ligand-binding domain of a first receptor selected from the group consisting of a low density lipoprotein receptor (LDLR), an acetylated LDLR, a transforming growth factor .beta. receptor, a cytokine receptor, an immunoglobulin Fc receptor, a hormone receptor, a glucose receptor, a glycolipid receptor, and a glycosaminoglycan receptor, wherein the ligand-binding domain binds the selected substance; andthe carrier domain comprises a glycosylated or unglycosylated amino acid sequence which (1) is not a portion of the first receptor, and (2) binds a cell surface receptor on the cell other than the first receptor,such that the chimeric protein binds to the selected substance and to the cell surface receptor on the cell, whereupon the cell surface receptor on the cell transports the chimeric protein and the selected substance into the cell.