Patent ID: 6726926
Filing Date: 2004-04-27
Classification: A61K,C12N

Abstract:
A gene-entrapping liposome preparation, which comprises:liposomes wherein plasmid DNA is entrapped in said liposomes, and a disaccharide as a protector of said liposome and said plasmid DNA entrapped therein, which disaccharide is present at a concentration ranging from 300 to 3333 g per 1 g of said plasmid DNA entrapped in said liposomes, wherein the preparation is a frozen product obtained by freezing of the gene-entrapping liposome preparation during which cleavage of the plasmid DNA is prevented by the presence of said disaccharide added; said liposomes are of a diameter ranging from 0.6 to 6 &mgr;m; and constitutive lipids of said liposomes are N-(&agr;-trimethylammonioacetyl)-didodecyl-D-glutamate chloride (TMAG), dilauroyl phosphatidylcholine (DLPC) and dioleoyl phosphatidylethanolamine (DOPE), whereby the plasmid DNA in the gene-entrapping liposome preparation remains uncleaved.