Patent ID: 8993292
Filing Date: 2015-03-31
Classification: C12M,C12N

Abstract:
1. A method comprising: (a) placing a biological sample in a first compartment of a system, wherein the first compartment comprises a cell-trapping matrix, wherein the compartment is exposed to a magnetic force external to the compartment; (b) capturing the sperm cells and the non-sperm cells with the cell-trapping matrix; (c) incubating the cell-trapping matrix and the captured sperm cells and non-sperm cells in a selective sperm lysis buffer to form a sperm cell lysate, the selective sperm lysis buffer comprising about 1 M KCl and 200 mM DTT; (d) binding sperm cell DNA from the sperm cell lysate to a plurality of DNA-binding particles; and (e) eluting the sperm cell DNA from the DNA-binding particles.