Patent ID: 7993889
Filing Date: 2011-08-09
Classification: C12N,C12P,C12Y,Y02E,Y02P

Abstract:
1. A method for producing isobutanol comprising; a. providing a fermentation media comprising carbon substrate; and b. contacting said media with a recombinant yeast microorganism expressing an engineered isobutanol biosynthetic pathway wherein said pathway comprises the following substrate to product conversions; and wherein a) the substrate to product conversion of step (i) is performed by an acetolactate synthase enzyme; b) the substrate to product conversion of step (ii) is performed by an acetohydroxy acid isomeroreductase enzyme; c) the substrate to product conversion of step (iii) is performed by an acetohydroxy acid dehydratase enzyme; d) the substrate to product conversion of step (iv) is performed by a decarboxylase enzyme; and e) the substrate to product conversion of step (v) is performed by an alcohol dehydrogenase enzyme; whereby isobutanol is produced.