Patent ID: 6767718
Filing Date: 2004-07-27
Classification: A61K,C07K

Abstract:
A method for preparing an intermediate for use in the synthesis of a lipodepsipeptide antibiotic, including the steps of:(a) Obtaining an A21978 complex having a cyclic depsipeptide unit by fermentation of Streptomyces roseosporus; (b) Protecting the orinithine amino group of the A21978 antibiotic complex by FMOC, the protective function 9-fluorenylmethoxycarbonyl; (c) Treating the product from the reaction of step (b) with a deacylase enzyme to provide a single protected peptide; and (d) Using a modified Edman degradation method or peptidase enzymatic reactions to obtain a protected intermediate in the form of a cdpeptide chosen from the group consisting of FMOC-cdpeptide-2, FMOC-cdpeptide-1, or FMOC-cdpeptide, by reducing in size by one to three amino acids in the exocyclic tripeptide side chain of the cyclic depsipeptide unit of said complex.