Patent ID: 8609358
Filing Date: 2013-12-17
Classification: C07K,C12Q,G01N

Abstract:
1. A method for detecting the presence or absence of a wound-specific bacterium in a sample selected from a wound, a body fluid or fluid from a wound, said method comprising the steps of: a) contacting said sample with a detectably labeled synthetic α 1-proteinase inhibitor reactive site loop domain peptide substrate selected from the group consisting of EAAGAMFLEAIPK (SEQ ID NO: 1), EGAMFLEAIPMSIPK (SEQ ID NO: 2), KGTEAAGAMFLEAIPMSIPPEVK (SEQ ID NO: 3), GAMFLEAIPMSIPPE (SEQ ID NO: 4), CGAMFLEAIPMSIPAAAHHHHH (SEQ ID NO: 5), and variants or fragments of any of said peptide substrates, wherein said variant or fragment of any of said peptide substrates has at least 40% sequence identity to any of said peptide substrates, under conditions that result in cleavage of said substrate by a protease enzyme produced in said sample by a wound-specific bacterium, wherein said peptide substrate is coupled to both a support and to at least one detectable moiety; and b) detecting a cleavage or an absence of the cleavage of the substrate, the cleavage of the substrate indicating the presence of the wound-specific bacterium in the sample and absence of the cleavage of the substrate indicating absence of the wound-specific bacterium in the sample.