Patent ID: 6277626
Filing Date: 2001-08-21
Classification: C12P

Abstract:
A method of separating enantiomeric lactam esters comprising contacting the lactam esters with a biocatalyst in an aqueous solution, an organic solvents, or a mixture of organic and aqueous solvents wherein only one enantiomer is selectively hydrolyzed to give the optically active isomer of the corresponding acid, and separating the hydrolysis product from the unreacted lactam esters, wherein the lactams are of Formula II: ##STR7##R.sup.1 is selected from the group consisting of alkyl, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, which may optionally be substituted by one or more of the following: alkyl, alkenyl, alkynyl, hydroxy, alkoxy, thiol, thioalkoxy, and halogen;L is selected from the group consisting of no group or alkylene, alkenylene, and alkynylene;wherein L may optionally be substituted by one or more of the following: alkyl, alkenyl, alkynyl, hydroxy, alkoxy, thiol, thioalkoxy, halogen, nitro, amino, alkylamino, dialkylamino, aminoalkyl, dialkylaminoalkyl, arylamino, aminoaryl, alkylaminoaryl, cyano, and haloalkyl;X is selected from the group consisting of (CH.sub.2).sub.p, and CH.dbd.CH;p=1 to 4;Y is selected from the group consisting of NH, O, (CH.sub.2).sub.q and CH.dbd.CH;q=1 to 2;Z is selected from the group consisting of (CH.sub.2).sub.v and CH.dbd.CH;v=2;R.sup.2, R.sup.3, and R.sup.4 are independently selected from hydrogen, alkyl, alkenyl, alkynyl, hydroxy, alkoxy, thiol, thioalkoxy, halogen, nitro, amino, alkylamino, dialkylamino, aminoalkyl, dialkylaminoalkyl, cyano, and haloalkyl, wherein all said substitutions may be optionally substituted with one or more of the following: halogen, alkyl, amino, alkylamino, dialkylamino, aminoalkyl, hydroxy, and alkoxy.