Patent ID: 6617115
Filing Date: 2003-09-09
Classification: G01N

Abstract:
A method of screening for a bioactive agent capable of binding to a domain of cellular proliferation protein KSP (SEQ ID NO:2), wherein said domain of said cellular proliferation protein (i) is KSP-L360 (amino acids 1-360 of SEQ ID NO:4), KSP-K491 (amino acids 1-491 of SEQ ID NO:6), KSP-S553 (amino acids 1-553 of SEQ ID NO:8), KSP-K368 (SEQ ID NO:10), or a fragment or a variant thereof, said variant having greater than 80% amino acid sequence identity to said domain of said cellular proliferation protein and (ii) has microtubule binding activity, said method comprising:(a) combining in vitro said domain of said cellular proliferation protein and a candidate bioactive agent, and (b) determining the binding of said candidate bioactive agent to said domain of said cellular proliferation protein, provided that said candidate bioactive agent is not a microtubule, ATP, or an antibody.