Patent ID: 6355457
Filing Date: 2002-03-12
Classification: C07K,C12P

Abstract:
An isolated nucleic acid fragment comprising a nucleotide sequence that encodes at least one polypeptide required for the biosynthesis of epothilone, wherein the complement of said nucleotide sequence hybridizes to a sequence selected from the group consisting of: nucleotides 54935-62254 of SEQ ID NO:1, nucleotides 55028-56284 of SEQ ID NO:1, nucleotides 56600-57565 of SEQ ID NO:1, nucleotides 57593-58087 of SEQ ID NO:1, nucleotides 60362-61099 of SEQ ID NO:1, nucleotides 61211-61426 of SEQ ID NO:1, and nucleotides 61427-62254 of SEQ ID NO:1, under conditions of hybridization at 6.15Â° C. for 36 hours and washing 3 times at high stringency with 0.1Ã—SSC and 0.5% SDS for 20 minutes at 65Â° C.