Patent ID: 6682942
Filing Date: 2004-01-27
Classification: B82Y

Abstract:
A method of screening an immobilized biological moiety for its ability to interact with an analyte contained within a fluid sample comprising the steps of:(i) providing a channel sample detection device, said sample detection device comprising: (a) a substrate having a substrate surface; (b) a channel formed in or on said substrate adjacent said substrate surface, said channel extending between first and second ends, and having a bottom wall surface defining a substantially planar immobilization region intermediate the first and second ends and opposing side walls; (c) a cover attached adjacent said substrate surface forming with said channel a closed channel having first and second ports adjacent said first and second ends; (d) a thin film having a hydrophilic display surface covering said immobilization region, said thin film being effective to resist non-specific protein binding; and, (e) at least one immobilization group capable of immobilizing said biological moiety, said immobilization group being attached to said thin film, wherein if said fluid sample containing said analyte is introduced into said channel and said analyte interacts with said biological moiety when such biological moiety is immobilized to said immobilization group to form said immobilized biological moiety, then such interaction is observable; (ii) contacting said fluid sample with said channel so that said fluid sample contacts said immobilized biological moiety; and, (iii) monitoring said interaction between said immobilized biological moiety and said analyte.