Patent ID: 8512954
Filing Date: 2013-08-20
Classification: C12Q

Abstract:
1. A method of detecting methicillin-resistant S. aureus (MRSA) in a sample, said method comprising: (a) providing a first set of primers, wherein said first set of primers are complementary to at least a portion of a mecA polynucleotide sequence; (b) providing a second set of primers, wherein said second set of primers are complementary to at least a portion of an MSSA-orfX polynucleotide sequence, wherein said MSSA-orfX polynucleotide sequence is from a region of the orfX gene surrounding the position at which an insertion cassette would be inserted in methicillin resistant (c) providing a third set of primers, wherein said third set of primers are complementary to at least a portion of an (d) combining said first, second and third set of primers with said sample in a reaction mixture; (e) performing a multi-cycle amplification reaction with said reaction mixture; (f) determining cycle numbers of appearance of each of said mecA, MSSA-orfX and