Patent ID: 6423885
Filing Date: 2002-07-23
Classification: C12N,C12Y

Abstract:
A method for reducing the phenotypic expression of a nucleic acid of interest, which is normally capable of being expressed in a plant cell, said method comprising the steps ofa) providing to the nucleus of said plant cell unpolyadenylated RNA comprising a target specific nucleotide sequence produced by transcription of a chimeric DNA comprised within said plant cell, said chimeric DNA comprising a plant-expressible promoter operably linked to a target specific DNA region encoding said RNA, said target-specific DNA region comprising a nucleotide sequence of at least 10 consecutive nucleotides having at least about 70% sequence identity to about 100% sequence identity to said nucleic acid of interest and wherein said chimeric DNA farther comprises a DNA region involved in 3â€² end formation and polyadenylation, preceded by a self-splicing ribozymne encoding DNA region; and b) selecting said plant cell wherein the phenotypic expression of said nucleic acid of interest is reduced.