Patent ID: 6187314
Filing Date: 2001-02-13
Classification: A61K,A61P,Y10S

Abstract:
A method for obtaining a Ginkgo biloba composition containing flavonoids and lactone, and determining the total amount of flavonoids therein comprising steps of:(a) obtaining dried Ginkgo biloba leaves;(b) breaking the leaves into small pieces;(c) putting the broken leaves through reflux in a solution selected from the group consisting of water, C1 to C3 alkanols, acetone, and a combination thereof under conditions permitting the extraction of flavonoids and lactones to produce an extract and a residue;(d) separating the extract from the residue;(e) concentrating the separated extract to a density of about 1.2 to about 1.25 at about 60.degree. C.;(f) applying the concentrate from step (e) to at least two kinds of resins under conditions permitting binding of flavonoids and lactones;(g) eluting the bound flavonoids and lactones, thereby producing an extract composition containing flavonoids and lactones from Ginkgo biloba; and(h) determining the total amount of the flavonoids in the eluted composition of step (g) comprising a standard curve assay calculation wherein rutin is used as the standard.