Patent ID: 7074984
Filing Date: 2006-07-11
Classification: A01H

Abstract:
1. A process for obtaining a cross-incompatible inbred maize plant comprising a gene cluster that encodes for a Teosinte Crossing Barrier (TCB) trait, which when crossed with a second inbred maize plant, produces a hybrid maize plant which is cross-incompatible and contains a gene cluster that encodes for a TCB trait, the process comprising the steps of: a) crossing a first donor parental maize plant comprising a gene cluster that encodes for a TCB trait with a second donor parental maize plant containing genes that encode for desirable traits in hybrid combination, wherein the gene cluster in said first donor parental maize plant comprises a Tcb locus and at least one modifier gene and further wherein said first donor parental maize plant is derived from a maize plant designated W22-TCB, seeds of which have been deposited as ATCC No. PTA-1601; b) collecting the seed resulting from the cross in step a); c) planting and growing the seed collected in step b) under plant growth conditions; d) obtaining DNA from each of the plant of the plant population grown in step c); e) analyzing the DNA obtained from each plant in step d) using at least one molecular marker including and between molecular markers umc1117 and bnlg490 shown in f) selecting plants from said population having the Tcb locus and at least one modifier gene for further crossings and screenings; g) repeating steps d)–f) until a line is obtained which is homozygous for the gene cluster for cross-incompatability to provide such a trait in an inbred maize plant to be used in hybrid combination; and h) selecting plants from said population having the gene cluster for cross-incompatibility for further crossings and screenings until a line is obtained which is homozygous for the gene cluster for cross-incompatibility to provide such a trait in an inbred maize plant to be used in hybrid combination.