Patent ID: 9115187
Filing Date: 2015-08-25
Classification: A61K,C07K,C12N,C12Q,G01N

Abstract:
1. A method of identifying a monoclonal antibody, or antigen-binding fragment thereof, that specifically binds at least two different lyssavirus species, comprising: screening a naïve antibody phage display library with at least two different cell lines, wherein each cell line expresses a different lyssavirus glycoprotein selected from the RABV glycoprotein, the MOKV glycoprotein, the WCBV glycoprotein, the LBV glycoprotein and the DUVV glycoprotein, and wherein the phage display library is screened sequentially on the at least two different cell lines; and selecting a phage display clone that specifically binds to at least two different lyssavirus species, glycoproteins from at least two different lyssavirus species, or both, thereby identifying a monoclonal antibody, or antigen-binding fragment thereof, that specifically binds at least two different lyssavirus species.