Patent ID: 6632619
Filing Date: 2003-10-14
Classification: B01F,B01J,B01L,B81B,Y10S

Abstract:
A method of observing the effect of one or more candidate compounds on cells in a microfluidic device comprising:(a) providing a microfluidic device comprising a main flow path comprising an outlet, at least two inlet flow paths and a detection zone, wherein at least one inlet flow path intersects and merges with the main flow path at the detection zone, wherein the intersection of inlet flow paths with the main flow path is at an upstream angle of less than 90Â°; (b) applying into the microfluidic device at least one cell which flows into a first inlet flow path and a candidate compound to a second inlet flow path; (c) inducing flow of the cells and the candidate compound toward the outlet; (d) allowing the at least one cell to mix with the candidate compound in the detection zone; and (e) observing the effect of the candidate compound on the cells in the detection zone.