Patent ID: 6800733
Filing Date: 2004-10-05
Classification: C07K,C12N,G01N,Y10S

Abstract:
A composition of matter comprising a modified form of an Aequorea wild-type GFP polypeptide, wherein the amino acid sequence of said modified form of an Aequorea wild-type GFP polypeptide is at least 95% homologous to the amino acid sequence of SEQ ID NO:2 and comprises a replacement of Ser at a position corresponding to position 202 in the wild-type GFP sequence by Phe and a replacement of Thr at a position corresponding to position 203 by lle and wherein, upon oxidation and cyclization of amino acid residues in the modified form corresponding to positions 65 to 67 of wild-type GEP polypeptide sequence (SEQ ID NO:2), a fluorescent polypeptide having two main excitation peaks, with increased fluorescence exhibited at a shorter-wavelength peak of the two main excitation peaks as compared with a corresponding polypeptide of the wild-type GFP polypeptide sequence is formed.