Patent ID: 9023640
Filing Date: 2015-05-05
Classification: C12Q,G01N

Abstract:
1. A system for rapidly detecting the presence of an infectious agent in a biological sample, comprising: a. a single biosensor reagent including at least one antibody specific for a predetermined infectious agent and a bioluminescent agent, wherein the at least one antibody is expressed on the surface of living, engineered B-lymphocytes and wherein the bioluminescent agent is aequorin expressed by the living, engineered B-lymphocytes, the biosensor reagent being operative to: (i) detect the presence of a specific infectious agent in a biological sample to be tested, and (ii) emit a detectable light signal when the biosensor reagent reacts with the biological sample and detects the presence of the specific infectious agent in the biological sample; b. a single-use, disposable test cartridge, wherein the single-use, disposable test cartridge includes: (i) a reservoir card, wherein the reservoir card further includes the biosensor reagent; (ii) a test cartridge base, wherein the test cartridge base is configured to accept the reservoir card and wherein the test cartridge base further includes: c. a testing unit adapted to receive the single-use, disposable test cartridge, the testing unit including a sensor for detecting the detectable light signal emitted by the biosensor reagent upon reacting with the biological sample, the detection of the emitted detectable light signal being indicative of the presence of the infectious agent in the biological sample and being the only analysis of the emitted detectable light signal that is performed, wherein detection of the specific infectious agent in the biological sample occurs in real time.