Patent ID: 6235509
Filing Date: 2001-05-22
Classification: C07K,C12N

Abstract:
A process for inhibiting serine proteases in a sample comprising the steps:(i) adding to said sample a compound of the general formula (I) and ##STR4##in whichX denotes C or N,R.sub.1 denotes H, methyl, ethyl or OH,R.sub.2 and R.sub.3 each denote H, methyl, ethyl, OH or residues which together form a ring closure with a maximum of 6 C atoms in the ring andR.sub.4 denotes H, OH or an optionally substituted alkyl, alkenyl or alkinyl group with up to 4 C atoms provided that at least one of R.sub.1, R.sub.2, R.sub.3 or R.sub.4 represents an OH group and the compound has a pKa of at least 8 and(ii) adding to said sample a compound selected from the group consisting of sulfonic acid derivatives and fluorophosphonates, to derivative said serine proteases,wherein said compound (I) avoids nonspecific derivatization of proteins other than said serine proteases.