Patent ID: 7479378
Filing Date: 2009-01-20
Classification: C12Q,G01N

Abstract:
1. A method for analyzing a protein sample containing a physiologically-acceptable digestive enzyme mixture with lipolytic, proteolytic and amylolytic activity by two-dimensional gel electrophoresis, wherein the mixture can be used in the manufacture of a pharmaceutical preparation, said method comprising: (a) preparing the protein sample by dissolving the sample in a gel electrophoresis solvent composition comprising a protein solving solvent, an internal standard for quantifying proteins, and a protease inhibitor; (b) defining the first dimension of the gel electrophoresis by isoelectric focusing, and applying a gradient to separate protein fractions; (c) re-buffering the protein fractions; (d) transferring the protein fractions from (c) to the second dimension of the gel electrophoresis and separating components of the fractions by SDS-PAGE; (e) fixing and staining SDS-PAGE gels resulting from (d); (f) evaluating the gels densitometrically by fluorescence scanning; and (g) determining the identity and absolute quantity of one or more proteins in the digestive enzyme mixture.