Patent ID: 7642060
Filing Date: 2010-01-05
Classification: C12Q

Abstract:
1. A method for detecting the presence or absence of a target pathogen in a sample, comprising: placing the sample in a sampling container with a resuscitation and/or growth medium, without performing a separate non-selective enrichment step, wherein the resuscitation and/or growth medium inside the sampling container comprises a selective growth medium; incubating the sample in the resuscitation and/or growth medium in the container, in the presence of a capture matrix, said capture matrix containing an antibody for the target pathogen, wherein the sample is incubated for a length of time and at a temperature suitable for resuscitation and/or growth of the target pathogen, and the capture matrix is present throughout the resuscitation and/or growth process; and removing said capture matrix from the resuscitation and/or growth medium in the container and performing a detection assay on said capture matrix removed from the resuscitation and/or growth medium to detect the target pathogen.