Patent ID: 6919311
Filing Date: 2005-07-19
Classification: A61K,A61P,C07K

Abstract:
1. A modified Factor VIII polypeptide having a Factor VIII:C activity, wherein (I) the modified Factor VIII polypeptide has at least one modification in at least one A3 domain portion, wherein the portion is selected from the group of portions, based upon the human sequence, consisting of amino acids (i) 1743 (Phe) to 1749 (Arg), (ii) 1784 (Ser) to 1831 (Asp), (iii) 1888 (Ser) to 1919 (His), (iv) 1942 (Trp) to 1947 (Met), and (v) 1959 (Ser) to 1974 (Ala), wherein the modification is an amino acid substitution, deletion or addition, and wherein the modification reduces the binding to low-density lipoprotein receptor protein (LRP), and (II) at least one modification in at least one C1 domain portion, wherein the portion is selected from the group of portions, based upon the human sequence, consisting of amino acids (i) 2037 (Ile) to 2062 (Trp), (ii) 2108 (Asp) and 2118 (Asn), and (iii) 2154 (Thr) to 2158 (Ile), wherein the modification is an amino acid substitution, deletion or addition.