Patent ID: 8080387
Filing Date: 2011-12-20
Classification: C07K,C12N,C12P

Abstract:
1. A method for preparing a recombinant protein that improves productivity, solubility and folding of a target protein, the method comprising: (a) inserting into a vector a gene encoding protein disulfide isomerase (PDI) as a fusion partner; and (b) ligating a gene encoding the target protein with said gene encoding PDI, wherein the amino-terminal domain of said PDI comprises a sequence of 6 amino acid residues KIEEGK (SEQ ID NO: 37) obtained from an amino-terminal domain of a maltose-binding protein, and wherein the recombinant protein comprises a sequence of 6 to 10 histidines at the amino-terminus or carboxy-terminus of the PDI protein, and a peptide sequence consisting of 6 to 10 aspartic acid and glutamic acid residues between said PDI and the target protein.