Patent ID: 7309611
Filing Date: 2007-12-18
Classification: B01L,G01N,Y10S,Y10T

Abstract:
1. A test strip adapted to receive a sample and detect an analyte therein, the test strip comprising: a buffer addition zone to which a buffer may be added; an absorbent zone positioned in a first flow direction relative to the buffer addition zone; one or more test zones positioned in a second, different, flow direction relative to the buffer addition zone, at least one of the test zones including a first analyte binding agent immobilized therein which is capable of binding to the analyte to be detected; a terminal buffer flow zone positioned in the second flow direction relative to the one or more test zones, the absorbent zone having an absorption capacity relative to the other zones of the test strip such that when a volume of buffer within a predetermined buffer volume range for the test strip is added to the buffer addition zone, the buffer initially diffuses from the buffer addition zone both in the first flow direction to the absorbent zone and in the second flow direction past the one or more test zones to a diffusion point within the terminal buffer flow zone and then reverses flow, and diffuses in the first flow direction past the one or more test zones toward the absorbent zone; and a sample addition zone positioned in the second flow direction relative to where the buffer reverses flow, to which a sample may be added.