Patent ID: 6337095
Filing Date: 2002-01-08
Classification: A61P,C07D

Abstract:
A process for the isolation of the compound scopoletin from Artemisia annua and other plant families, said process comprising:a) extracting dried powdered material of different plant parts with an aqueous acetonitrile solvent for 6 to 8 hours, wherein a ratio of the plant parts to the aqueous acetonitrile solvent is 1:5, b) concentrating the extracted solvent up to 30% by volume of its original extract under vacuum, c) partitioning the concentrated extract with halogenated solvent to transfer scopoletin in the non-polar halogenated solvent, d) drying the halogenated solvent over anhydrous sodium sulphate and evaporating the solvent, e) crystallizing residues from step (d) with a solvent and filtering the crystals to obtian a filtrate, f) concentrating the filtrate, g) subjecting the concentrated filtrate of step (f) to silica gel chromatography, h) eluting scopoletin from the concentrated filtrate of step (g) in a chloroform/methanol mixture, i) identifying the fractions containing the eluted scopolctin, and j) crystallizing the fractions containing scopoletin to obtain a purified scopolctin compound.