Patent ID: 8426157
Filing Date: 2013-04-23
Classification: C12M,C12Q,G01N

Abstract:
1. A method for analyzing the effect of a gaseous medium on a biological test system using an extracellular metabolization system, comprising the following steps: cultivating a biological test system on a permeable carrier; placing the permeable carrier with the biological test system into an exposure device in such a manner that compartments are formed within the exposure device, the compartments being physically separated from one another by the permeable carrier such that there is an exposure atmosphere on one side of the permeable carrier and a conservation medium with the extracellular metabolization system on the other side of the permeable carrier; guiding the gaseous medium over the surface of the biological test system to form the exposure atmosphere over the biological test system; adding the extracellular metabolization system to the conservation medium; and positioning the conservation medium with the extracellular metabolization system under the permeable carrier and in contact with the permeable carrier, in such a way that the extracellular metabolization system only passes through the permeable carrier, while the biological test system is not flooded with extracellular metabolization system; wherein the conservation medium with extracellular metabolization system contained therein is routed through a delivery and a removal system having a controlled overflow regulation for a desired level of said conservation medium with extracellular metabolization system in the exposure device.