Patent ID: 6153416
Filing Date: 2000-11-28
Classification: C02F,C12N,Y02W

Abstract:
A process for preparing microbial cells or enzymes immobilized in beads, said process comprising the steps of:i) preparing an aqueous solution by mixing 1-14 weight percent calcium alginate, 1-14 weight percent polyethylene glycol and 1-14 weight percent polyethylene imide with water;ii) mixing the aqueous solution from step i) with 6 ml of a concentrated solution of a microorganism or an enzyme to form a mixture;iii) subjecting the mixture to a gelation treatment and hardening treatment by contacting the mixture with 4-8% w/v CaCl.sub.2 for 3-4 hours to form spherical beads; andiv) rinsing the spherical beads with water for about 2-3 minutes, and then transferring the spherical beads to water in a mixer and mixing for 6-9 hours to obtain said microbial cells or enzymes immobilized in beads.