Patent ID: 8475743
Filing Date: 2013-07-02
Classification: B01F,B01L,B33Y,C12Q,F16K,G01N,Y10T

Abstract:
1. A microfluidic device, comprising: a first flow channel formed in a first layer of a substrate, the first flow channel is in fluid communication with a first chamber; a first control channel formed in a second layer of a substrate, wherein the second layer is adjacent to the first layer; and a second flow channel formed in a third layer of a substrate, wherein the third layer is adjacent to the second layer; a reaction channel formed in the third layer, wherein the reaction channel is in fluid communication with the second flow channel, wherein a first portion of the reaction channel is in fluid communication with the first chamber, wherein a second portion of the reaction channel is in fluid communication with a second chamber, wherein an interface valve is disposed along the reaction channel to isolate the first portion from the second portion; and a first isolation valve and a second isolation valve, each disposed along the first control channel, wherein a change in pressure within the first control channel actuates the first isolation valve to isolate the first chamber from a portion of the first flow channel and simultaneously actuates the second isolation valve to isolate the second portion of the reaction channel from the second flow channel.