Patent ID: 7785874
Filing Date: 2010-08-31
Classification: C12N,C12Q

Abstract:
1. A method for monosynaptic transport of a heterologous nucleic acid sequence, comprising: contacting one or more primary neurons, each of which is connected by a plurality of synapses to a plurality of secondary neurons, with: wherein the secondary neurons do not contain the nucleic acid molecules encoding one or more polypeptides that complement in trans the TST-defective phenotype of the TST-defective virus, under conditions that permit expression of the polypeptide(s) in the primary neuron(s), rescue of the TST-defective phenotype by the polypeptide(s) in the primary neuron(s), and thereby monosynaptically transporting the TST-defective virus comprising the heterologous nucleic acid sequence from the primary neuron(s) to the plurality of secondary neurons across the plurality of synapses.