Patent ID: 7612257
Filing Date: 2009-11-03
Classification: C12N

Abstract:
1. An isolated nucleic acid molecule comprising a sequence of nucleotides encoding a flavonoid 3′,5′ hydroxylase (F3′5′H), said nucleotide sequence selected from the group consisting of: (i) a nucleotide sequence encoding the amino acid sequence as set forth in SEQ ID NO: 12; (ii) a nucleotide sequence encoding an amino acid sequence having at least 95% identity to the amino acid sequence as set forth in SEQ ID NO: 12; (iii) the nucleotide sequence as set forth in SEQ ID NO: 11; and (iv) a nucleotide sequence capable of hybridizing to the complementary sequence of the nucleotide sequence as set forth in SEQ ID NO: 11 under high stringency conditions which comprise hybridization at 42° C. in 50% v/v formamide, 1M NaCl, 1% w/v SDS, and 10% w/v dextran sulphate, and washings at 65° C. first in 2×SSC, 1% w/v SDS and subsequently in 0.2×SSC, 1% w/v SDS.