Patent ID: 6204050
Filing Date: 2001-03-20
Classification: C12N,C12P,C12R

Abstract:
A process to produce diastereomers of 4-hydroxyproline comprising:(a) contacting, in an aqueous reaction mixture, at least one starting material selected from the group consisting of trans-4-hydroxy-L-proline and cis-4-hydroxy-D-proline with an enzyme catalyst in a form selected from the group consisting of whole cells, cell extracts, partially purified enzyme or purified enzyme, the enzyme catalyst characterized by 4-hydroxyproline epimerase activity and derived from Acinetobacter baumanni WT-50/50-4A (ATCC 202144) or Serratia marcescens WT-L-4A1 (RED) (ATCC 202145); and(b) isolating trans-4-hydroxy-L-proline and cis-4-hydroxy-D-proline from the reaction mixture.