Patent ID: 6017712
Filing Date: 2000-01-25
Classification: C07H

Abstract:
A labeled nucleotide having the formula ##STR34## wherein: B is a 7-deazapurine, purine, or pyrimidine nucleotide base;W1 and W2 taken separately are selected from the group consisting of H and OH;W3 is selected from the group consisting of is OH, --PO4, --P2O7, --P3O10, and analogs thereof,wherein when B is purine or 7-deazapurine, the sugar moiety is attached at the N9-position of the purine or deazapurine, and when B is pyrimidine, the sugar moiety is attached at the N1-position of the pyrimidine;wherein if B is a purine, the linkage is attached to the 8-position of the purine, if B is 7-deazapurine, the linkage is attached to the 7-position of the 7-deazapurine, and if B is pyrimidine, the linkage is attached to the 5-position of the pyrimidine;wherein D is a dye compound having the formula ##STR35## wherein R1-R6 taken separately are selected from the group consisting of hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group, and combinations thereof, or, when taken together, R1 and R6 is benzo, or, when taken together, R4 and R5 is benzo;Y1-Y4 taken separately are selected from the group consisting of hydrogen and lower alkyl, or, when taken together, Y1 and R2, Y2 and R1, Y3 and R3, and/or Y4 and R4 is propano, ethano, or substituted forms thereof;X1-X3 taken separately are selected from the group consisting of hydrogen, chlorine, fluorine, lower alkyl, carboxylate, sulfonic acid, --CH2OH and linking group; andthe linkage linking B and D is attached to D at one of positions R1-R6 or X1-X3.