Patent ID: 8168432
Filing Date: 2012-05-01
Classification: C12M,C12N

Abstract:
1. A method of culturing cells comprising: a. forming a cell culture device that is not compartmentalized by a semi-permeable membrane in which to perform cell culture, at least a portion of said cell culture device being comprised at least in part of non curved, non porous gas permeable material; b. adding a volume of medium and adding cells into said cell culture device, said cells being an animal cell type and further being of a suspension cell type; c. orienting said cell culture device into a cell gravitation position and allowing at least a portion of said cells to gravitate onto at least a portion of said non curved, non porous gas permeable material and making any adjustments needed to place said medium at a depth such that the highest location of said medium is beyond 2.0 cm above the lowest location of said medium when said cell culture device is residing in said cell gravitation position; and d. placing said cell culture device in a cell culture location that includes ambient gas at a composition for cell culture, wherein said cell culture device is oriented in a position such that at least a portion of said cells reside upon at least a portion of said non curved, non porous gas permeable material.