Patent ID: 8216833
Filing Date: 2012-07-10
Classification: C12N

Abstract:
1. A cosmid vector comprising: (1) an adenoviral genome having a left-inverted terminal repeat and a right-inverted terminal repeat, each of said repeats having a complete nucleotide sequence, (2) a deletion in an adenovirus E1 gene region, wherein the E1 gene deletion site comprises a restriction enzyme recognition sequence at the E1 gene deletion site, (3) a first pair of identical restriction enzyme recognition sequences, wherein said first pair of sequences are not naturally present in the adenoviral genome, one of said sequences in the first pair is located on the left side of the left-inverted terminal repeat and the other of said sequences in the first pair is located on the right side of the right-inverted terminal repeat; (4) a drug resistant gene, a replication origin, a spacer sequence and a COS region in this order from outside of the left-inverted terminal repeat sequence toward the right inverted terminal repeat sequence, (5) a second pair of identical restriction enzyme recognition sequences, wherein one of said sequences in the second pair is located at the E1 gene deletion site and at a right side of the restriction enzyme recognition sequence in (2), and the other of said sequences in the second pair is present inside the spacer sequence.