Patent ID: 7833769
Filing Date: 2010-11-16
Classification: C12Q,Y10T

Abstract:
1. An isolated E. coli McrA variant able to cleave DNA comprising methylcytosine but not hydroxymethylcytosine, the variant comprising an amino acid sequence which differs from the amino acid sequence of its parent E. coli McrA polypeptide solely by: (a) a modification at a position corresponding to amino acid 67 of SEQ ID NO: 2; or (b) a modification at a position corresponding to amino acid 67 of SEQ ID NO: 2 and at one or more positions selected from the group consisting of those corresponding to amino acids 3, 15, 20, 58, 60, 74, 118, 128, 157, 158, 159, 167, 174, and 177 of SEQ ID NO: 2.