Patent ID: 7579156
Filing Date: 2009-08-25
Classification: C12Q,G01N

Abstract:
1. A method for measuring the activity of a phospholipase, the method comprising the steps of: providing a liposome consisting of: 1) a nonfluorescent phosphatidylcholine (PC) wherein the nonfluorescent PC is dioleoyl PC (DOPC), 2) a nonfluorescent, negatively charged molecule wherein the nonfluorescent, negatively charged molecule is phosphatidylglycerol (PG) and 3) a fluorescently labeled molecule wherein the fluorescently labeled molecule is 1,2-bis-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-undecanoyl)-sn-glycero-3-phosphocholine (BODIPY-PC), wherein hydrolization of the phospholipid components of the liposome by the phospholipase causes a fluorescence intensity change; contacting the phospholipase with the liposome; and detecting the fluorescence intensity change due to the hydrolization of the phospholipid components of the liposome by the phospholipase to determine the activity of the phospholipase.