Patent ID: 6015891
Filing Date: 2000-01-18
Classification: C07K,C12N,Y02A

Abstract:
A synthetic Bacillus thuringiensis (B.t.) gene which is expressed in descendant plant cells and encodes a pesticidal protein toxin, wherein said synthetic B.t. gene is produced by the process of:selecting a B.t. pesticidal protein toxin desired to be expressed in a plant cell;obtaining a table indicating codon usage bias for a gene or genes more highly expressed in a plant cell than a native B.t. gene;using said table to design a modified coding sequence which encodes said protein toxin, whereby said modified coding sequence has a frequency of codon usage that more closely resembles the frequency of codon usage of the plant cell in which it is to be expressed than did the native B.t. coding sequence encoding said protein toxin, said modified coding sequence having at least about 10% of the nucleotides changed as compared to the native B.t. coding sequence;obtaining a synthetic B.t. gene comprising a coding region comprising said modified coding sequence wherein said coding region is under the control of a plant-expressible promoter;introducing said synthetic B.t. gene into a plant cell;culturing said cell to obtain descendant plant cells or plants comprising descendant plant cells, said descendant plant cells comprising said synthetic B.t. gene; andestablishing that said synthetic B.t. gene is expressed in said descendant plant cells.