Patent ID: 8216806
Filing Date: 2012-07-10
Classification: C07K,C12N,Y02A

Abstract:
1. A modified Cry3A toxin comprising a non-naturally occurring protease recognition site, wherein said protease recognition site modifies a Cry3A toxin and is located at a position selected from the group consisting of: a. between amino acids corresponding to amino acid numbers 536 and 542 of SEQ ID NO:4; and b. between amino acids corresponding to amino acid numbers 107 and 111 of SEQ ID NO:4, and between amino acids corresponding to amino acid numbers 536 and 541 of SEQ ID NO:4, wherein said protease recognition site is recognizable by a gut protease of western corn rootworm, and wherein said modified Cry3A toxin causes higher mortality to western corn rootworm than the mortality caused by said Cry3A toxin to western corn rootworm in an artificial diet bioassay.