Patent ID: 6015679
Filing Date: 2000-01-18
Classification: G01N,Y10S

Abstract:
A process for measuring human complement activity, which comprises:reacting liposomes having a size within the range of 100 to 500 nm,the size being a mean particle size in nm plus twice the standard deviation, which is obtained and calculated from a particle size distribution measured by a dynamic light scattering method, said liposomes also having an encapsulated marker therein and an immobilized hapten on surface of the liposome membranes capable of reacting with an unbound antibody,with the unbound antibody to the hapten and a sample containing human complement, whereby said liposomes are ruptured by reaction with the complement;measuring an amount of liberated marker according to the degree of rupture of the liposomes andmeasuring human complement activity in the sample on the basis of the amount of liberated marker thus obtained.