Patent ID: 6344345
Filing Date: 2002-02-05
Classification: C12N,Y10T

Abstract:
A method for producing double-stranded DNA comprising:providing single-stranded DNA comprising a homopolymer at one or both ends; employing said single-stranded DNA to produce a double-stranded DNA comprising a homopolymer at one or both ends; wherein the double-stranded DNA does not contain internal type IIS restriction sites or is chemically modified in order to protect the double-stranded DNA from internal type IIS restriction enzyme cleavage; after producing said double-stranded DNA, treating said double-stranded DNA comprising a homopolymer at one or both ends with a type IIS restriction enzyme to partly or fully eliminate at least one of the homopolymers, wherein the restriction enzyme is capable of cleaving said double-stranded DNA at a cleavage site separate from the recognition site therefor.