Patent ID: 7217552
Filing Date: 2007-05-15
Classification: C12N,C12P,G01N

Abstract:
1. An isolated and purified nucleic acid encoding a polypeptide having an activity of degrading a sulfated fucogalactan containing galactose and fucose as constituent saccharides in a molar ratio of 1:1 to 6:1, which is selected from the group consisting of: (a) a nucleic acid encoding a polypeptide that comprises the amino acid sequence of SEQ ID NO:28; (b) a nucleic acid comprising the nucleotide sequence of SEQ ID NO:27; and (c) a nucleic acid capable of hybridizing to the nucleic acid of (b) or a complete complementary strand thereof under the stringent conditions of 6×SSC (1×SSC: 0.15M NaCl, 0.015M sodium citrate, pH 7.0) containing 0.5% SDS, 5×Denhardt's reagent, 100 μg/ml denatured, fragmented salmon sperm DNA at 68° C. for 12 to 20 hours when immobilized on a membrane, and washing in 2×SSC containing 1% SDS at room temperature while changing the SSC concentration down to 0.1×SSC and the temperature up to 68° C. until a signal from the immobilized nucleic acid can be distinguished from background.