Patent ID: 9051610
Filing Date: 2015-06-09
Classification: C12Q

Abstract:
1. An RNA in situ hybridization method for identifying a presence of an mRNA of a target gene in a tissue sample consisting of the steps of: (i) hybridizing the tissue sample with one or more oligonucleic acid probes labeled with a low-molecular-weight compound on at least one of the bases of the one or more oligonucleic acid probes, which hybridizes to the mRNA of the target gene expressed in the tissue sample and one or more different dummy oligonucleic acids that are substantially equal in length with the one or more oligonucleic acid probes, wherein the one or more different dummy oligonucleic acids neither hybridize with the mRNA of the target gene expressed in the tissue sample nor with the oligonucleic acid probes, and wherein the one or more dummy oligonucleic acids are adsorbed to sites of the tissue sample where the one or more of the oligonucleic acid probes are non-specifically adsorbed and prevent non-specific adsorption of one or more of the oligonucleic acid probes to the tissue sample and (ii) detecting the low-molecular-weight compound with an antibody enzyme conjugate that binds to the low-molecular-weight compound and amplifying a signal using a color-developing compound or a fluorescent molecule compound as a substrate for the enzyme, and further detecting the signal by a 10× to 40× objective lens; thereby identifying the presence of the mRNA of the target gene.