Patent ID: 6380366
Filing Date: 2002-04-30
Classification: A61K,A61Q,Y10S

Abstract:
A method of preparing a fractionated cartilage extract comprising water soluble components having a molecular weight of less than about 500 kDa, the method comprising the step of:fractionating a crude cartilage extract comprising water soluble components obtained from cartilage material, said water soluble components having at least one biological activity selected from the group consisting of anti-angiogenic, anti-inflammatory, anti-collgenolytic, anti-tumor, anti-metalloproteasic, anti-elastase, inhibition of VEGF activity, anti-acne, inhibition of telangiectesia, inhibition of endothelial cell proliferation in mammalian skin, inhibition of melanoma in mammalian skin, inhibition of expression of rosacea in mammalian skin, inhibition of papulosquamous skin disease in mammalian skin, reduction of the appearance of peri-orbital dark circles in mammalian skin, inhibition of activated-keratinocyte differentiation in mammalian skin, inhibition of expression of eczema in mammalian skin, reduction of warts in mammalian skin, promotion of wound repair in mammalian skin, and promotion of the skin barrier function in mammalian skin, wherein said fractionating removes said water soluble components having a molecular weight of greater than about 500 kDa, thereby to form a first fractionated cartilage extract.