Patent ID: 6083686
Filing Date: 2000-07-04
Classification: C12N

Abstract:
A gene for Taq polymerase wherein the sequence of the first thirty nucleotide bases in the native gene which code for the first ten amino acids in the mature native protein, has been changedA) by substituting therefor a modified nucleotide sequence selected from the group consisting of:SEQ ID NO: 2: ATG CGT GGT ATG CTG CCT CTG TTT GAG CCG AAG,SEQ ID NO: 4: ATG GAC TAC AAG GAC GAC GAT GAC AAG CGT GGT ATG CTG CCC CTC TTT GAC CCC AAG, orB) by inserting between the start codon (ATG) of the mature native protein and the codon, (AGG) for the second amino acid of the mature native protein, the sequence:SEQ ID NO: 8: GAC TAC AAG GAC GAC GAT GAC AAG.