Patent ID: 8895321
Filing Date: 2014-11-25
Classification: G01N,Y10S

Abstract:
1. A method for detecting the presence of a target in a fluid sample, the method comprising: introducing a fluid sample to a microfluidic device including a reaction region, a detection region, and a microfluidic channel therebetween; exposing the fluid sample to a plurality of labeled receptors reversibly immobilized within the reaction region under conditions suitable for the receptors to specifically bind any target present in the fluid sample; releasing the receptors from the reaction region under conditions such that a substantial portion of the bound target remains bound to the released receptors; transporting the released receptors through the microfluidic channel such that bound and unbound receptors separate based on differential electrokinetic mobilities; and detecting a signal produced by the arrival of the bound and unbound receptors to the detection region.