Patent ID: 7790427
Filing Date: 2010-09-07
Classification: C12N

Abstract:
1. A method of treating biocells comprising the steps of: a. providing biocells; b. applying at least one stressor to the biocells sufficient to cause nonlethal and reparable cell wall damage to the biocells, thereby putting the biocells in a catabolic state during which catabolic metabolic functions predominate over anabolic metabolic functions; and c. obtaining at least one product produced by the biocells during the catabolic state, wherein the stressor is a pulsed electric field of between 2 kV/cm to less than 15 kV/cm, applied for between 5 and 20 microseconds, with each electric field pulse lasting about 0.2 to about 5 microseconds, at a total energy input of between about 1 to 10 J/ml.