Patent ID: 6100055
Filing Date: 2000-08-08
Classification: A61K,C07K,Y02A

Abstract:
An isolated DNA molecule coding for a f.alpha..sub.2 M-binding protein or polypeptide obtained by:(a) partially digesting chromosomal DNA from streptococcal cells whereby DNA fragments are obtained;(b) ligating said DNA fragments into expression vectors;(c) packaging said expression vectors containing said ligated DNA into phage particles;(d) infecting E. coli cells with said phage particles to obtain a plurality of phage clones;(e) selecting from said plurality of phage clones the phage clones which react with f.alpha..sub.2 M;(f) and purifying the phage DNA from said selected phage clones to obtain said isolated DNA molecule,wherein said DNA molecule comprises the nucleotide sequence of SEQ ID NO:3, or a part thereof, the nucleotide sequence of SEQ ID NO:5, or a part thereof, or the nucleotide sequence of SEQ ID NO:7, or a part thereof, and wherein said DNA molecule encodes a protein or polypeptide with f.alpha..sub.2 M-binding activity.