Patent ID: 6323042
Filing Date: 2001-11-27
Classification: G01N,Y10S

Abstract:
A flow-through immunosensor comprising:an entrance port for receiving a fluid buffer, said entrance port being machined in or molded and integrated with a monolithic substrate;a microcapillary passage machined in or molded and integrated with said monolithic substrate, said microcapillary passage having an inner diameter of less than about 1 mm and having antibodies immobilized on its inner wall, fluorescently labeled analogs of an antigen being immunologically bound to and saturating antigen-binding sites of said immobilized antibodies;a sample introduction port in fluid connection with said microcapillary passage;a detection cell downstream from and in fluid connection to said sample introduction port and joined to said microcapillary passage, said detection cell including;at least one window transparent to a wavelength of light that fluorescently excites said label, andat least one window transparent to a wavelength of light emitted by said excited label; andan exit port machined in and integral with said monolithic substrate, said exit port being downstream from and in fluid connection with said detection cell, said exit port providing a path for spent buffer and label to exit said monolithic substrate.