Patent ID: 6746848
Filing Date: 2004-06-08
Classification: G01N,Y10S

Abstract:
A method of preparing calibration slides for a cell imaging densitometer, comprising the steps of:(a) immobilizing cultured cells in a hydrophilic matrix, wherein said hydrophilic matrix is an aqueous gel of a polymer selected from the group consisting of proteins (polypeptides), oligosaccharides, and poly(acrylamide); (b) placing the matrix in molten paraffin; (c) cooling the molten paraffin until it solidifies; (d) exposing the solidified paraffin containing the immobilized cells to a degree of intervening fixation less than produced by fixation with 10% normal buffered for four hours; and (e) sectioning the solidified paraffin containing the immobilized cells into at least one thin slice suitable for optical microscopy.