Patent ID: 6127334
Filing Date: 2000-10-03
Classification: A61K,Y10S

Abstract:
A method for preparing an insulin product, comprising the steps of:(a) providing an acidic solution comprising an insulin analogue, a source of zinc, and a sub-isophanic amount of protamine, wherein the weight ratio of the insulin analogue to protamine in the acidic solution results in a weight ratio of dissolved to precipitated insulin analogue in the range of 20:80 to 80:20 in the insulin product;(b) providing an alkaline solution comprising a substance which acts as a buffer at physiological pH; wherein at least one of the solutions of steps (a) and (b) further comprises a phenolic compound;(c) mixing the acidic and alkaline solutions to form a suspension; and(d) leaving the suspension to form the insulin product which comprises a soluble phase comprising the insulin analogue and a solid phase comprising the precipitated insulin analogue.