Patent ID: 8017837
Filing Date: 2011-09-13
Classification: C12N

Abstract:
1. A method for producing transgenic oil palm, the method comprising: (a) treating a target tissue using plasmolyzing medium (PM) which contains 4% to 10% of sucrose and 100 μM to 300 μM of Acetosyringone (AS) and gold particles, wherein the target tissue comprises embryogenic calli from oil palm and the embryogenic calli are bombarded with the gold particles; (b) infecting the target tissue with a bacterial suspension of a suitable (c) treating the infected target tissue in a PM having 4% to 10% sucrose and 100 μM to 300 μM AS and for a period of time of between 1 to 3 days; (d) cultivating the infected target tissue in a co-cultivation medium (CM) that contains 100 μM to 300 μM of AS, wherein the cultivating is carried out in a dark condition at a temperature of between 25° C. to 30° C. for a period of between 1 to 5 days; (e) treating the infected target tissue with a non-selection medium including an antibiotic, wherein the treating is carried out under light at a temperature of between 25° C. to 30° C. for a period of between 1 week to 1 month; treating the transformed target tissue with a selection medium including a herbicide, the herbicide including phosphinothricin (PPT) as an active ingredient, wherein the treating is carried out under light at a temperature of between 25° C. to 30° C. and sub cultured to select for a transformant comprising the transgene; (g) regenerating the putative transformant comprising the transgene; and (h) analysing the number of copies of the transgenes.