Patent ID: 6599700
Filing Date: 2003-07-29
Classification: A01K,A61K,A61P,C07H,C12N,C12Q,G01N

Abstract:
A method for detecting transition single-nucleotide polymorphisms at CpG sites in a target nucleic acid molecule, comprising:a) providing a detectably labeled wild type or mutant target nucleic acid containing CpG sequences; b) contacting said wild type or mutant target nucleic acid with a homologous nucleic acid sequence isolated from a test subject, under conditions whereby heteroduplexes form between said sequences; c) contacting said heteroduplex with MED1 protein, wherein said MED1 protein is encoded by a nucleic acid selected from the group consisting of SEQ ID NOs: 1, 5, 22, 23, 25, 26, and 27, thereby forming a complex between the heteroduplex and MED1 and exposing said complex to hot alkali, said exposure resulting in cleavage of any heteroduplexes containing a G:T mismatch on the T-containing strand; and d) detecting said cleavage product, if any.