Patent ID: 8932813
Filing Date: 2015-01-13
Classification: C07H,C12N,C12Q,C12Y

Abstract:
1. A method for producing an unblocked oligonucleotide from a blocked oligonucleotide comprising a non-extendable 3′ end removable by polyphosphorolysis, the method comprising contacting the blocked oligonucleotide hybridized to a target nucleic acid with one or more polyphosphorolyzing agents selected from the group consisting of: wherein n and/or m are selected from the group consisting of 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, and 10, with the proviso that n and m cannot both be 0, and in the presence of an enzyme having polyphosphorolysis activity and removing the 3′ terminal nucleotide of the blocked oligonucleotide to produce the unblocked oligonucleotide.