Patent ID: 9057102
Filing Date: 2015-06-16
Classification: C12N,C12Q,G01N,G16B

Abstract:
1. A method of performing a nucleic acid sequencing reaction, comprising: a) preparing a reaction mixture comprising components of the nucleic acid sequencing reaction including a polynucleotide to be sequenced; b) introducing the reaction mixture to a membrane comprising a nanopore; c) initiating the nucleic acid sequencing reaction in the reaction mixture to begin progression of the nucleic acid sequencing reaction, wherein the nucleic acid sequencing reaction comprises passage of the polynucleotide or a nascent strand complementary thereto through the nanopore and monitoring the nanopore as voltage is applied across the membrane; and d) maintaining conditions that allow the nucleic acid sequencing reaction to proceed while subjecting the reaction mixture to at least two detection periods and at least one non-detection period during the progression of the nucleic acid sequencing reaction, wherein the progress of the polynucleotide or the nascent strand through the nanopore is slowed during the detection periods to allow sequence determination, and further wherein the progress of the polynucleotide or the nascent strand through the nanopore is sped up during the non-detection period, thereby performing the nucleic acid sequencing reaction.