Patent ID: 8192932
Filing Date: 2012-06-05
Classification: C07H,C12N,C12Y

Abstract:
1. A method of covalently joining a DNA strand to an RNA strand comprising: (a) forming a vaccinia topoisomerase-DNA intermediate by incubating a DNA cleavage substrate comprising a vaccinia topoisomerase cleavage site with a vaccinia topoisomerase specific for that site, wherein the vaccinia topoisomerase-DNA intermediate has one or more 5′ single-strand tails; and (b) adding to the vaccinia topoisomerase-DNA intermediate an acceptor RNA strand complementary to the 5′ single-strand tail under conditions permitting a ligation of the covalently bound DNA strand of the vaccinia topoisomerase-DNA intermediate to the RNA acceptor strand and dissociation of the vaccinia topoisomerase, thereby covalently joining the DNA strand to the RNA strand.