Patent ID: 8912392
Filing Date: 2014-12-16
Classification: A01H,C12N,C12Q

Abstract:
1. A method of modifying a specific endogenous genomic target sequence comprising: (a) contacting at least one monocot plant cell comprising the endogenous genomic target sequence in its genome with (i) a phenotypic marker, (ii) a DNA fragment, and (iii) an engineered double-strand break inducing agent capable of inducing a double-strand break at the endogenous genomic target sequence, wherein the engineered double-strand break inducing agent comprises SEQ ID NO:21; (b) selecting cells comprising the phenotypic marker wherein the phenotypic marker is not indicative of integration at the endogenous genomic target sequence; and (c) identifying cells from step (b) having an alteration in their genome at the endogenous genomic target sequence wherein the alteration is selected from the group consisting of (i) replacement of at least one nucleotide, (ii) a deletion of at least one nucleotide, (iii) an insertion of at least one nucleotide, and (iv) any combination of (i)-(iii), wherein identifying comprises any method that does not use the phenotypic marker.