Patent ID: 8637286
Filing Date: 2014-01-28
Classification: C12N,C12P,Y02E

Abstract:
1. A non-naturally occurring microbial organism comprising: (1) at least one exogenous nucleic acid encoding an enzyme expressed in a sufficient amount to enhance carbon flux through acetyl-CoA, wherein said at least one exogenous nucleic acid encodes an enzyme selected from the group consisting of: (2) (a) an isopropanol pathway that converts acetyl-CoA to isopropanol, wherein the isopropanol pathway comprises 1) an acetoacetyl-CoA thiolase; 2) an acetoacetyl-CoA transferase, an acetoacetyl-CoA hydrolase, an acetoacetyl-CoA synthetase, or a phosphotransacetoacetylase/acetoacetate kinase; 3) an acetoacetate decarboxylase; and 4) an isopropanol dehydrogenase; (3) a reductive TCA (rTCA) pathway, wherein said microbial organism comprises at least one exogenous nucleic acid encoding a rTCA pathway enzyme expressed in a sufficient amount to enhance carbon flux through acetyl-CoA, wherein said rTCA enzyme is selected from the group consisting of 1) an ATP-citrate lyase, 2) a citrate lyase, 3) a fumarate reductase, and 4) an alpha-ketoglutarate:ferredoxin oxidoreductase.