Patent ID: 7625565
Filing Date: 2009-12-01
Classification: A61K,C07K,G01N

Abstract:
1. A composition comprising a protein obtained by extraction of a lysine-rich histone fraction from non-human mammalian thymus cell nuclei by pepsin enzymatic degradation resulting in the formation of peptides having substantially reduced sizes compared to the undegraded thymus cell nuclei, said composition comprising an unmethylated protein of about 35 kilo dalton as determined by 11% non-reducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a methylated protein of about 28 kilodalton as determined by 11% nonreducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and having properties recited in the following (a) and (b) on a two-dimensional electrophoretic gel: (a) ability to precipitate a single continuous precipitation adjacent to β, α1-, α2-macro globulins from serum of a healthy human; and (b) inability to precipitate a single continuous precipitation adjacent to β, α1-, α2-macro globulins from serum of an AIDS patient, the composition being useful for the treatment and diagnosis of HIV infection.