Patent ID: 7335762
Filing Date: 2008-02-26
Classification: B01L,B82Y,C12P,C12Q,G01N

Abstract:
1. A cuvette for simultaneously monitoring and recording an individual light emission in an array of reaction chambers comprising: (a) a detection chamber, comprising an opening which comprises an array comprised from a cavitated fiber optic wafer formed from a fused bundle of a plurality of individual optical fibers, wherein each individual optical fiber having a diameter between 3 and 100 μm, the wafer comprising a top surface and a bottom surface, the top surface comprising at least 400,000 reaction chambers, wherein said reaction chambers are etched into the top surface of the cavitated fiber optic wafer and wherein the thickness of the wafer between the top surface and the bottom surface is between 0.5 mm and 5.0 mm in thickness; wherein the depth of each reaction chamber ranges from between one half the diameter of an individual optical fiber and three times the diameter of an individual optical fiber; said detection chamber further comprising at least one surface or opening that allows transmission of said light emission, said reaction chamber adapted to receive at least one analyte; (b) a receptacle within said detection chamber to align said array with an optical detector; and (c) affluent and effluent outlets connected to said detection chamber to allow said array to be in fluid communication with one or more reagent reservoirs.