Patent ID: 6043032
Filing Date: 2000-03-28
Classification: C12N

Abstract:
A method of extracting single or double-stranded DNA or RNA from a viable sample or microorganism culture comprising consisting essentially of the steps of:(a) mixing the sample with a carrier which is at least one member selected from the group consisting of dextran, acrylamide and carboxymethyl cellulose to form a liquid mixture;(b) mixing said liquid mixture with reagent C to render both the single or double-stranded DNA or RNA and the carrier insoluble and coprecipitate them, said reagent C containing at least one reagent A selected from the group consisting of guanidinium thiocyanate, guanidiniumn hydrochloride, potassium thiocyanate and sodium thiocyanate in a concentration sufficient to disrupt and solubilize cell membranes, wherein the final concentration of reagent A is 1.5 to 3.0M, and at least one reagent B selected from the group consisting of n-propyl alcohol, isopropyl alcohol, n-butyl alcohol, sec-butyl alcohol, term-butyl alcohol and tert-amyl alcohol; and(c) separating the coprecipitated insolubilized DNA or RNA and carrier from the liquid phase.