Patent ID: 8202693
Filing Date: 2012-06-19
Classification: C12N

Abstract:
1. A method of isolating nucleic acids from a biological sample of cells, comprising: a) contacting a sample containing cells with a cell membrane lysis solution in the presence of a solid support coated with negative charge, wherein the cell membrane lysis solution selectively lyses cell membranes over nuclear membranes, and wherein said solid support coated with negative charge binds to intact cell nuclei; b) separating intact cell nuclei bound solid support from cell membrane lysis supernatant; c) contacting the intact cell nuclei bound to said solid support with a nuclear membrane lysis solution to lyse nuclear membranes, wherein nucleic acids are released from lysed nuclei and bind to said solid support; and d) contacting the lysed nuclear membranes and nucleic acid bound solid support with a precipitant to precipitate said nucleic acid bound solid support.