Patent ID: 8603548
Filing Date: 2013-12-10
Classification: A61K,A61P

Abstract:
1. An anti-avian influenza virus agent comprising, as an active ingredient, a guava component, wherein the guava component is obtained by a process comprising: extracting guava leaves with an extraction solvent consisting primarily of at least one of water and a hydrophilic organic solvent to form an extract; subjecting the extract to high performance liquid chromatography fractionation by first applying the extract to an ODS column having an inside diameter of 20 mm and a length of 250 mm, and adding a water-methanol mixture having a methanol concentration of 20% as a mobile phase to the ODS column during an initial period of 15 minutes after applying the guava extract to the ODS column; adding a water-methanol mixture having a methanol concentration of 40% as a mobile phase to the ODS column during a subsequent period of 15 to 30 minutes; adding a water-methanol mixture having a methanol concentration of 60% as a mobile phase to the ODS column during a subsequent period of 30 to 45 minutes; eluting the guava component from the ODS column over a period of 0 to 20 minutes after applying the guava extract to the column, and/or over a period of 20 to 35 minutes after applying the guava extract to the column; and contacting the guava component with polyvinylpolypyrrolidone to adsorb and remove polyphenols therefrom.