Patent ID: 7951539
Filing Date: 2011-05-31
Classification: C12Q

Abstract:
1. A system to detect one or more nucleic acids of interest, the system comprising: A) a first bDNA amplification reaction comprising: a first solid support capable of capturing the one or more nucleic acids of interest; one or more amplification oligomers comprising a first sequence complimentary to a repeat sequence of one or more first amplification multimers; and, the one or more first amplification multimers comprising a plurality of the repeat sequences complementary to the first sequence of at least one of the amplification oligomers, and comprising one or more sequences complimentary to the one or more nucleic acids of interest or to a label extender complimentary to the nucleic acids of interest, whereby hybridization of the components in the presence of the one or more nucleic acids of interest provides one or more complexes binding one or more of the amplification oligomers to the solid support; and, B) a second bDNA amplification reaction comprising a capture extender or a capture probe comprising a sequence complimentary to a second sequence of the one or more amplification oligomers; and comprising a label extender, a second amplification multimer or preamplifier complimentary to a third sequence of the one or more amplification oligomers; wherein detection of the one or more amplification oligomers by the second bDNA amplification reaction indicates the presence of the one or more nucleic acids of interest in the first bDNA amplification reaction.