Patent ID: 6064474
Filing Date: 2000-05-16
Classification: G01N

Abstract:
A method for determining hematocrit of a blood sample, comprising the steps of:measuring absorbance of said blood sample at a first isobestic wavelength;measuring absorbance of said blood sample at a second isobestic wavelength; anddetermining hematocrit of said blood sample in accordance with the algorithm:Hct=(T.sub.i,1 -R*T.sub.i,2)/(K.sub.1 -R*K.sub.2),wherein:T.sub.i is total absorbance at an isobestic wavelength;K is a constant that includes a molar extinction coefficient Hb at that wavelength, a red blood cell scattering factor, and path length;Hct is hematocrit; andR is the ratio of light scattering from a plasma component measured at two wavelengths.