Patent ID: 7846732
Filing Date: 2010-12-07
Classification: C12N

Abstract:
1. A method of promoting sequence specific recombination of a first DNA and a second DNA in a eukaryotic cell comprising: (a) providing the eukaryotic cell wherein the eukaryotic cell comprises the first DNA comprising an attB, an attP, an attL or an attR sequence; (b) introducing into the cell the second DNA comprising the attB, the attP, the attL or the attR sequence, wherein if said first DNA comprises the attB sequence, said second DNA comprises the attB, the attL or the attR sequence, or wherein if said first DNA comprises the attP sequence, said second DNA comprises the attP, the attL or the attR sequence, or wherein if said first DNA comprises the attL sequence, said second DNA comprises the attB, the attP or the attL sequence, or wherein if said first DNA comprises the attR sequence, said second DNA comprises the attB, the attP or the attR sequence; and (c) providing to the cell comprising the first DNA and the second DNA a bacteriophage lambda integrase Int under conditions that promote integrase-mediated sequence specific recombination between the first DNA and the second DNA.