Patent ID: 6114513
Filing Date: 2000-09-05
Classification: A61K,C07H,C07J,C12N,C12Q,C12Y

Abstract:
A method comprising the step of contacting a biological sample suspected of containing an RNA with a compound comprising a plurality of linked nucleosides, wherein:each nucleoside comprises a ribofuranosyl sugar portion and a base portion; andat least one of said nucleosides bears at a 2'-O-position, a 3'-O-position, or a 5'-O-position a substituent having formula:--R.sub.S --S--R.sub.1where:R.sub.S has formula R.sub.A, R.sub.A --C(O)--Q--R.sub.A, R.sub.A --Q--R.sub.A --Q--R.sub.A ;each R.sub.A is independently selected from alkyl having from 1 to about 10 carbon atoms;each Q is independently selected from the group of NH, O, and S;R.sub.1 is H or a thiol protecting group or has formula S--R.sub.2, CH.sub.2 C(O)--NH--R.sub.2, CH.sub.2 --CH.dbd.CH--C(O)--R.sub.2, --CH.sub.2 --CH.sub.2 --NH--S(O).sub.2 --R.sub.2, or (maleimido)-R.sub.2 ; andR.sub.2 comprises a steroid molecule, a reporter molecule, a lipophilic molecule, a reporter enzyme, a peptide, a protein, a reporter group, an alkylator, an intercalator, a cell receptor binding molecule, a crown ether, a crown amine, a porphyrin, a crosslinking agent, a peptide nucleic acid, or a thiol attached to a poly(ethylene glycol).