Patent ID: 8263393
Filing Date: 2012-09-11
Classification: C12N

Abstract:
1. A method for expressing a heterologous polypeptide in a cell, said method comprising: culturing a transfected cell clone to allow expression of the heterologous polypeptide, wherein cells of the transfected cell clone contain integrated into their genomes a transfected nucleic acid molecule comprising: wherein the transfected nucleic acid molecule comprising the promoter functionally linked to an open reading frame encoding the heterologous polypeptide is flanked on one or both sides by a Stabilizing Anti-Repressor (STAR) molecule comprising a nucleic acid molecule selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, and SEQ ID NO:7.