Patent ID: 7713748
Filing Date: 2010-05-11
Classification: G01N,Y10S

Abstract:
1. A method for detecting an analyte in a test sample, the method comprising: i) providing an assay device that comprises: wherein the detection zone and the calibration zone are located downstream from the sampling pad and the conjugate pad; ii) contacting the assay device with the test sample, wherein a quantity of the analyte in the test sample less than or equal to a predefined base quantity binds to the first capture reagent at the scavenging zone and a quantity of the analyte in excess of the predefined base quantity binds to the specific binding member of the detection probes to form complexes that flow through the porous membrane and bind to the second capture reagent in the detection zone to generate a detection signal, and wherein the detection probes, the calibration probes, or a combination thereof, flow through the porous membrane and bind to the third capture reagent at the calibration zone to generate a calibration signal; iii) detecting the intensity of the detection signal and the calibration signal; and iv) comparing the intensity of the detection signal to the intensity of the calibration signal, wherein the quantity of the analyte within the test sample in excess of the predefined base quantity is proportional to the intensity of the detection signal calibrated by the intensity of the calibration signal.