Patent ID: 7915026
Filing Date: 2011-03-29
Classification: C12N,C12P

Abstract:
1. A transformed host cell that synthesizes an isoprenoid or an isoprenoid precursor via a mevalonate pathway, wherein the transformed host cell is a prokaryote that does not normally synthesize isopentenyl pyrophosphate (IPP) through the mevalonate pathway, wherein the transformed host cell comprises one or more nucleic acids heterologous to the host cell, wherein the one or more heterologous nucleic acids comprises nucleotide sequences that encode two or more mevalonate pathway enzymes, and wherein said two or more mevalonate pathway enzymes comprises an enzyme that condenses two molecules of acetyl-CoA to acetoacetyl-CoA as the first step in the synthesis of the isoprenoid or isoprenoid precursor, and one or more additional mevalonate pathway enzymes selected from: (a) an enzyme that condenses acetoacetyl-CoA with acetyl-CoA to form 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA); (b) an enzyme that converts HMG-CoA to mevalonate; (c) an enzyme that phosphorylates mevalonate to mevalonate 5-phosphate; (d) an enzyme that converts mevalonate 5-phosphate to mevalonate 5-pyrophosphate; and (e) an enzyme that converts mevalonate 5-pyrophosphate to isopentenyl pyrophosphate, wherein culturing of said transformed host cell in a suitable medium provides for production of the two or more enzymes and synthesis of the isoprenoid or isoprenoid precursor in a recoverable amount of at least about 1 mg/L.