Patent ID: 6432642
Filing Date: 2002-08-13
Classification: C12Q

Abstract:
A binary composition for hybridizing to a target polynucleotide sequence comprising:a probe comprising a target-specific portion and a clamp-specific portion wherein the target-specific portion is capable of sequence-specific binding to a target polynucleotide sequence; and a clamp comprising two probe-specific portions, one or more labels and at least one nucleic acid analog selected from a nucleobase analog, a sugar analog, and an internucleotide analog; wherein the probe is hybridized to the clamp by sequence-specific binding of the probe-specific portion of the clamp to the clamp-specific portion of the probe, and the clamp is incapable of sequence-specific binding to the target polynucleotide; and wherein the two probe-specific portions are capable of sequence-specific binding to the clamp-specific portion of the probe to form a triplex.