Patent ID: 6320100
Filing Date: 2001-11-20
Classification: C07K,C12N,Y02A

Abstract:
A synthetic DNA coding sequence that encodes a Bacillus thuringiensis (Bt) insecticidal protein selected for optimized expression in a plant, wherein said synthetic DNA coding sequence is produced by a method comprising:(a) obtaining the amino acid sequence of said Bt insecticidal protein;(b) reverse-translating said amino acid sequence into a synthetic DNA coding sequence comprising a sufficient number of the single codons that most frequently encode each amino acid in maize, wherein said synthetic DNA coding sequence has at least about 60% G+C content, and wherein the single codons that most frequently encode each amino acid in maize are determinable by(i) pooling a plurality of gene sequences from maize,(ii) calculating a codon usage profile from said pooled maize gene sequences, and(iii) determining which single codon most frequently encodes each amino acid in maize; and(c) synthesizing said DNA coding sequence.