Patent ID: 6066611
Filing Date: 2000-05-23
Classification: A61K,A61Q,C11D,G06Q

Abstract:
A bleaching composition comprising:(a) from about 0.0001% to about 10% by weight of the cleaning composition of a protease enzyme which is a carbonyl hydrolase variant having an amino acid sequence not found in nature, which is derived from a precursor carbonyl hydrolase consists of a substitution of a different amino acid for a plurality of amino acid residues at a position in said precursor carbonyl hydrolase equivalent to position +76 in Bacillus amyloliquefaciens subtilisin, in combination with one or more amino acid residue positions equivalent to those selected from the group consisting of +99, +101, +103, +104, +107, +123, +27, +105, +109, +126, +128, +135, +156, +166, +195, +197, +204, +206, +210, +216, +217, +218, +222, +260, +265, and/or +274 in Bacillus amyloliquefaciens subtilisin, provided that:1) when said carbonyl hydrolase variant includes a substitution of amino acid residues at positions equivalent to +76 and +195, there is also a substitution of an amino acid residue at one or more amino acid residue positions equivalent to those selected from the group consisting of +99, +101, +103, +104, +107, +123, +27, +105, +109, +126, +128, +135, +156, +166, +197, +204, +206, +210, +216, +217, +218, +222, +260, +265, and/or +274;2) when said carbonyl hydrolase variant includes a substitution of amino acid residues at positions equivalent to +76 and +104, there is also a substitution of an amino acid residue at one or more amino acid residue positions equivalent to +99, +101, +103, +107, +123, +27, +105, +109, +135, +156, +195, +197, +204, +210, +216, +222, +260, +265, and/or +274;3) when said carbonyl hydrolase variant includes a substitution of amino acid residues at positions equivalent to +76 and +126, there is also a substitution of an amino acid residue at one or more amino acid residue positions equivalent to +99, +101, +103, +107, +123, +27, +105, +109 +135, +156, +195, +197, +204, +210, +216, +222, +260, +265, and/or +274;4) when said carbonyl hydrolase variant includes a substitution of amino acid residues at positions equivalent to +76 and +128, there is also a substitution of an amino acid residue at one or more amino acid residue positions equivalent to +99, +101, +103, +107, +123, +27, +105, +109, +135, +156, +195, +197, +204, +210, +216, +222, +260, +265, and/or +274;5) when said carbonyl hydrolase variant includes a substitution of amino acid residues at positions equivalent to +76 and +166, there is also a substitution of an amino acid residue at one or more amino acid residue positions equivalent to +99, +101, +103, +107, +123, +27, +105, +109, +135, +156, +195, +197, +204, +210, +216, +222, +260, +265, and/or +274;6) when said carbonyl hydrolase variant includes a substitution of amino acid residues at positions equivalent to +76 and +206, there is also a substitution of an amino acid residue at one or more amino acid residue positions equivalent to +99, +101, +103, +107, +123, +27, +105, +109, +135, +156, +195, +197, +204, +210, +216, +222, +260, +265, and/or +274;7) when said carbonyl hydrolase variant includes a substitution of amino acid residues at positions equivalent to +76 and +217, there is also a substitution of an amino acid residue at one or more amino acid residue positions equivalent to +99, +101, +103, +107, +123, +27, +105, +109, +135, +156, +195, +197, +204, +210, +216, +222, +260, +265, and/or +274;8) when said carbonyl hydrolase variant includes a substitution of amino acid residues at positions equivalent to +76 and +218, there is also a substitution of an amino acid residue at one or more amino acid residue positions equivalent to +99, +101, +103, +107, +123, +27, +105, +109, +135, +156, +195, +197, +204, +210, +216, +222, +260, +265, and/or +274;(b) a bleaching agent which either is an organic peroxyacid or is a combination of a bleach activator and a peroxygen compound capable of yielding hydrogen peroxide that can react with the activator to form an organic peroxyacid in situ in a bleaching solution formed from the composition; and(c) one or more cleaning composition materials compatible with the protease enzyme and bleaching agent.