Patent ID: 6323394
Filing Date: 2001-11-27
Classification: A01H,C12N

Abstract:
A method for regenerating a large number of viable and fertile transformed mint plants by tissue culture technique starting from a small tissue of mint plant, said method comprising:(i) cutting one or more internodal segments of mint plants;(ii) removing any contaminants which are potentially harmful to said method from the surface of the internodal segments;(iii) culturing the decontaminated internodal segments from step (ii) in a first medium capable of producing an organogenic callus, said first medium comprising:(a) salts,(b) vitamins,(c) a carbon source,(d) at least one plant growth regulator, and(e) a gelling agentat a pH in the range of 5.4 to 6.2 and sterilized by autoclaving, wherein the culturing is at 20-35.degree. C. in the presence of cool white light;(iv) continuing the culture of said internodal segments until callus and proliferating shoots are formed;(v) harvesting the shoots formed;(vi) culturing the shoots obtained from step (v) in a second medium capable of inducing roots, said second medium comprising:(a) salts,(b) vitamins,(c) a carbon source,(d) at least one plant growth regulator, and(e) a gelling agentat a pH in the range of 5.4 to 6.2 and sterilized by autoclaving, wherein the culturing is at 20-35.degree. C. in the presence of cool white light for a minimum period of two weeks to generate roots; and(vii) transforming said organogenic callus, explant or regenerated shoots using infection with Agrobacterium or bombardment with DNA coated microparticles,thereby resulting in the regeneration of said transformed callus, explant, or regenerated shoot by tissue culture techniques into a viable and fertile transformed mint plant.