Patent ID: 9081008
Filing Date: 2015-07-14
Classification: G01N

Abstract:
1. A method for determining the percent of asymmetrically self-renewing dividing cells in a sample of cells comprising: a. contacting a sample of cells with an antibody against an asymmetric self-renewal-associated (ASRA) protein to form an antibody-protein complex, wherein an antibody-protein complex is formed in situ and wherein the ASRA protein is a pattern-specific asymmetrically located protein in a cell undergoing asymmetrical cell kinetics selected from the group consisting of CXCR-6, BTG2, LGR-5 and H2A.FZ; b. detecting sister cell pairs of dividing cells or binucleated cells; c. detecting the antibody-protein complexes in sister cell pairs or binucleated cells, wherein the binucleated cells are arrested in cytokinesis; d. counting the total number of cells in the sample; e. counting the number of dividing cell comprising the pattern-specific asymmetrically localized antibody-protein complex; and f. computing the percent of asymmetrically self-renewing dividing cells in the sample by dividing the number of step e with the number of step d.