Patent ID: 7390646
Filing Date: 2008-06-24
Classification: A61K,C12N,Y02A

Abstract:
1. A bacterial delivery system comprising a live, attenuated bacterium comprising a DNA expression vector, which DNA expression vector comprises a nucleotide sequence encoding an exogenous RNA, wherein the nucleotide sequence is operably linked to a heterologous promoter that is functional in the bacterium, wherein said bacterium synthesizes the exogenous RNA encoded by the DNA vector, wherein the bacterium comprises an asd mutation, resulting in an impaired ability to synthesize a cell wall component, and an orgA mutation, resulting in reduced ability to induce apoptosis in the host eukaryotic cell, wherein the bacterium is a Salmonella strain, a Shigella strain, an Eseherichia coli strain, a Rickettsia strain, or a Listeria strain, and wherein, following infection of a eukaryotic host cell, the bacterium releases the exogenous RNA into the cytoplasm of the eukaryotic host cell.