Patent ID: 9193974
Filing Date: 2015-11-24
Classification: C12N

Abstract:
1. A process for chromosomal engineering, which comprises the steps of: 1) providing a bacterium having a DNA repair mechanism, for reassembling its fragmented DNA following a radiation and/or desiccation and/or chemical treatment, said mechanism comprising a step of extended synthesis dependent strand annealing (ESDSA) and a step of RecA dependent homologous recombination; 2) inducing the DNA repair mechanism by submitting the bacterium to radiation, desiccation and/or chemical treatment liable to damage the DNA, so as to substantially shatter its chromosomes into short fragments, said DNA repair mechanism comprising: annealing complementary single strand tails extended by an extended synthesis dependent strand annealing (ESDSA) templated on partially overlapping DNA fragments of said shattered chromosomes; and converting the resulting long linear DNA intermediates into intact circular chromosomes, by a RecA dependent homologous recombination; 3) inserting into the shattered chromosomes at least one foreign natural DNA or foreign synthetic DNA so as to become an integral part thereof during the process of DNA repair of step 2); and 4) optionally separating and collecting the recombined chromosomes thus obtained, wherein the process is carried out in a bacterium of the