Patent ID: 8951776
Filing Date: 2015-02-10
Classification: C12N,C12P,C12Y,Y02E

Abstract:
1. An isolated oleaginous yeast cell, comprising a genetic modification that increases expression of a diacylglycerol acyltransferase (DGA1) gene product and an acetyl-CoA carboxylase (ACC1) gene product, wherein the genetic modification comprises a first nucleic acid construct comprising an expression cassette comprising a coding nucleic acid encoding the DGA1 gene product; and a second nucleic acid construct comprising an expression cassette comprising a coding nucleic acid encoding the ACC 1 gene product; wherein the first nucleic acid construct and the second nucleic acid construct further comprises an intron-enhanced promoter that comprises a promoter sequence, a transcription start site, and an intronic sequence downstream of the transcription start site, and wherein the promoter is a translation elongation factor-1α (TEF) promoter.