Patent ID: 7786295
Filing Date: 2010-08-31
Classification: C12N

Abstract:
1. A method for isolating DNA from a biological sample comprising animal cells comprising the following sequential steps: (a) separating the cells comprising DNA from the remainder of the biological sample; (b) contacting the separated cells comprising DNA of step (a) with a hypertonic, high salt reagent having a concentration of salt therein so as to form a suspension of said biological cells; (c) contacting the suspension of step (b) with a lysis reagent so as to lyse the biological material containing DNA to form a lysate comprising DNA and non-DNA biological components of the biological material, wherein the hypertonic, high salt reagent in step (b) comprises salt in an amount effective to precipitate proteins out of the lysate; and (d) separating the DNA from the non-DNA biological components of the lysate of step (c) to yield isolated DNA.