Patent ID: 7575863
Filing Date: 2009-08-18
Classification: C07H,C12N,C12P,C12Q

Abstract:
1. A method for detecting a micro RNA (miRNA), wherein the miRNA is 18-25 ribonucleotides in length, comprising; hybridizing the miRNA and a linker probe, wherein the linker probe comprises a stem, a loop, and a 3′ target-specific portion, wherein the 3′ target-specific portion base pairs with a 3′ end region of the miRNA; extending the linker probe to form an extension reaction product; amplifying the extension reaction product in a polymerase chain reaction comprising a detector probe and a reverse primer that hybridizes to the linker probe or a complementary sequence to the linker probe, to form an amplification product, with the proviso that the linker probe is a different molecule from the detector probe, wherein the detector probe hybridizes to a nucleotide of the linker probe stem in the amplification product or hybridizes to a nucleotide of a complementary sequence of the linker probe stem in the amplification product; and, detecting the miRNA.