Patent ID: 8587786
Filing Date: 2013-11-19
Classification: G01N,G02B

Abstract:
1. A surface plasmon resonance microscope, comprising a radiation source emitting essentially monochromatic radiation, a sensor surface reflecting said essentially monochromatic radiation with a reflectivity to obtain a reflected radiation with an intensity, an optical assembly for illuminating said sensor surface with said radiation emitted by said radiation source in such a way that surface plasmons are generated in said sensor surface, a detector with a plurality of detector elements detecting said intensity of said reflected radiation, and an optical assembly for imaging said radiation reflected by said sensor surface on said detector wherein said resolution of said observing optical assembly and of said detector are higher than the diffraction-limited resolution which can be obtained with the radiation source achievable under classical conditions, a change of said reflectivity caused by a particle at said sensor surface is observed, the time dependency of said intensity in the range of the image of the particle is measured to quantify the signal of a particle, wherein the bonding of the particle at the sensor surface causes a saltatory intensity change at the corresponding image points, and the signal is normalized with respect to a reference image which is obtained by calculating the average image over a period of time before the particle is bound.