Patent ID: 7736901
Filing Date: 2010-06-15
Classification: C12Q,G01N,Y10T

Abstract:
1. A method of detecting a reaction product in a sample of blood or blood derivative using a cartridge that includes a holding chamber, an overflow chamber, an analysis location, a pump, a reagent and a sensor, the method comprising the steps of: (a) introducing the sample into the holding chamber in the cartridge; (b) a portion of the sample is retained in the holding chamber to form a metered sample and excess sample flows into the overflow chamber; (c) moving the metered sample from the holding chamber to the analysis location by pumping through the overflow chamber, wherein the overflow chamber is disposed between the pump and the holding chamber; (d) mixing the metered sampled with the reagent in the analysis location; (e) allowing the reagent to form the reaction product in the sample; (f) after the mixing step, positioning the sample at the sensor in the analysis location using the pump; (g) detecting the reaction product at the sensor; and (h) sealing the holding chamber with a closable sample entry port after step (a).