Patent ID: 6645753
Filing Date: 2003-11-11
Classification: A61P,C12N,C12Y

Abstract:
An isolated polynucleotide that encodes a dual specificity phosphatase-5 (DSP-5) substrate trapping mutant polypeptide in which a DSP-5 polypeptide comprising a sequence set forth in SEQ ID NO:3 has a substitution of an amino acid residue selected from the group consisting of (i) the aspartic acid residue at position 84 of SEQ ID NO:3 and (ii) the cysteine residue at position 115 of SEQ ID NO:3, such that the DSP-5 substrate trapping mutant polypeptide retains the ability to bind a DSP-5 substrate, and such that the ability of the DSP-5 substrate trapping mutant polypeptide to dephosphorylate the DSP-5 substrate is reduced relative to the DSP-5 polypeptide.