Patent ID: 7179655
Filing Date: 2007-02-20
Classification: G01N,Y10T

Abstract:
1. A method for determining the presence, amount, or activity of one or more active target proteins in a complex protein mixture, the method consisting of the sequential steps of: (a) contacting said complex protein mixture with a single activity based probe that specifically binds predominantly to a single target site on one or more active target proteins; (b) optionally removing from said complex protein mixture one or more components of said complex protein mixture not bound to said probe; (c) proteolyzing said active target protein(s) to produce a product mixture; (d) optionally binding said proteolyzed target protein(s) to a solid support; (e) separating said product mixture into two or more components, one or more of which consist essentially of peptides bound to said probe; and thereafter (f) generating a signal from said peptides bound to said probe, wherein said signal is correlated to the presence, amount, or activity of said one or more active target proteins in said complex protein mixture.