Patent ID: 7820407
Filing Date: 2010-10-26
Classification: A61P,C12N

Abstract:
1. A genetically modified Thraustochytriales microorganism for producing short chain fatty acids by a biosynthetic process, wherein the microorganism comprises a nucleic acid molecule encoding a fatty acid synthase and wherein the nucleic acid molecule encoding the fatty acid synthase has been modified to increase the expression of the fatty acid synthase, the fatty acid synthase comprising an amino acid sequence selected from the group consisting of: a) an amino acid sequence selected from the group consisting of: SEQ ID NO:2, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, an amino acid sequence consisting of positions 1-500 of SEQ ID NO:2, an amino acid sequence consisting of positions 450-1300 of SEQ ID NO:2, an amino acid sequence consisting of positions 1250-1700 of SEQ ID NO:2, an amino acid sequence consisting of positions 1575-2100 of SEQ ID NO:2, an amino acid sequence consisting of positions 2025-2850 of SEQ ID NO:2, an amino acid sequence consisting of positions 2800-3350 of SEQ ID NO:2, an amino acid sequence consisting of positions 3300-3900 of SEQ ID NO:2, an amino acid sequence consisting of positions 3900-4136 of SEQ ID NO:2, and a biologically active fragment thereof wherein the biologically active fragment is selected from the group of consisting of acetyl-transferase (AT) activity; enoyl ACP reductase (ER) activity; dehydrase (DH) activity; malonyl/palmitoyl acyltransferase (M/PAT) activity; a first acyl carrier protein (ACP) activity; a second acyl carrier protein (ACP) activity; keto-acyl ACP reductase (KR) activity; keto-acyl ACP synthase (KS) activity; and phosphopantetheinyl transferase (PPT) activity; and b) an amino acid sequence that is at least 85% identical to any of the amino acid sequences of (a) and having the biological activity of the amino acid sequence of (a).