Patent ID: 7919266
Filing Date: 2011-04-05
Classification: G01N,Y10T

Abstract:
1. A process for the detection of antibodies in a test sample containing serum or plasma, said process comprising; (a) preparing a suspension of erythrocytes with a test serum or plasma by mixing the test serum or plasma with erythrocytes; (b) incubating the suspension formed in step (a) at a temperature of from 37° C. to 45° C. for a period of time from sixty seconds to 10 minutes to bind any antibodies in the test serum or plasma to antigens on the surface of said erythrocytes; (c) combining said suspension in step (b) with an amount of a solution of a polycarboxylic acid macromolecule which is poly [(isobutylene-alt-maleic acid)NH4-co(isobutylene-alt-maleic anhydride)] (PIMA) used at a concentration of 0.1 to 5 wv % which potentiates the agglutination of said erythrocytes; (d) centrifuging the suspension of step (c); and, (e) determining the presence of anti-erythrocyte antibodies by detecting for occurrence of erythrocyte agglutination in the suspension.