Patent ID: 7276347
Filing Date: 2007-10-02
Classification: C07K,G01N

Abstract:
1. An immunoassay for detecting in a sample the presence of the active metabolites of cyclophosphamide, which can exist in the form of a free aldehyde containing compound of the formula: comprising treating said sample to protect the free aldehyde in said compound in the form of a protected aldehyde of the formula: wherein ═R forms an aldehyde protecting group, providing a mixture containing 1) said treated sample, 2) an antibody which is substantially selectively reactive with said protected aldehyde and substantially not cross reactive with cyclophosphamide and 3) a conjugate of a carrier with a ligand of the formula: wherein R is as above; Y is an organic spacing group; X is a terminal functional group capable of binding to said carrier; n is an integer of from 1 to 6; and p is an integer of from 0 to 1, causing, in said mixture, the protected aldehyde present in said treated sample and said conjugate to bind with said antibody and thereafter measuring the amount of said conjugate in said mixture which is bound to said antibody wherein the presence of the active cyclophosphamide metabolites in the sample can be determined.