Patent ID: 7109392
Filing Date: 2006-09-19
Classification: C12N

Abstract:
1. A method for increasing the oleic acid content in plant seeds, comprising the steps of: a) introducing a recombinant nucleic acid construct into a plant, said construct comprising at least one seed-specific regulatory sequence operably linked in sense orientation to a full length delta-12 fatty acid desaturase coding sequence, wherein said sequence encodes a delta-12 fatty acid desaturase protein having a substitution of a Lys residue for Asp or Glu in an amino acid region selected from the group consisting of His-Glu-Cys-Gly-His (SEQ ID NO:53), His-Asp-Cys-Gly-His (SEQ ID NO;55), and His-Asp-Cys-Ala-His (SEQ ID NO:54), b) obtaining progeny from said plant, said progeny producing said seeds having an oleic acid content of from about 72.5% to about 78.6%.