Patent ID: 7413659
Filing Date: 2008-08-19
Classification: B01D,G01N

Abstract:
1. A method of performing preparatory liquid chromatography comprising: drawing at least first and second solvents into a plurality of pumps from at least a corresponding first and second source of solvents; pumping said at least first and second solvents from said plurality of pumps; said step of drawing said at least first and second solvents including the step of mixing said at least first and second solvents together without using a separate mixer whereby a gradient for a preparatory liquid chromatographic system is formed; said step of mixing including the step of mixing said at least first and second solvents in said plurality of pumps during a refill portion of a pump cycle prior to pumping said at least first and second solvents from said plurality of pumps during a delivery portion of the pump cycle; said step of mixing further including the step of drawing said first and second solvents through at least one flow path, wherein the at least one flow path is shaped to produce good axial mixing and poor transverse mixing by stretching out flow streams of each of said first and second solvents; and said step of mixing further including the step of drawing said at least first and second solvents into a pump cylinder that is dimensioned with respect to the at least one flow path for good transverse mixing at a rate and pressure that causes turbulence where it undergoes enhanced transverse mixing and axial mixing from eddy currents in the pump cylinder.