Patent ID: 8247219
Filing Date: 2012-08-21
Classification: B01J,B01L,C12Q,C40B,G01N,Y10S,Y10T

Abstract:
1. A method for reacting a target nucleic acid and detecting an optical signal, comprising: providing a substrate comprising a chamber, wherein the chamber is pre-loaded with a first unlabeled oligonucleotide such that the first unlabeled oligonucleotide is immobilized on the substrate, and the chamber is pre-loaded with a second unlabeled oligonucleotide such that the second unlabeled oligonucleotide is immobilized on the substrate; introducing a target nucleic acid sequence into the chamber; reacting the target nucleic acid sequence with both the first unlabeled oligonucleotide and the second unlabeled oligonucleotide in the chamber, to form a first reaction product in the chamber; reacting the first reaction product with an optically detectable detection reagent in the chamber to form a second reaction product; and detecting an optical signal produced in the chamber by the second reaction product in the chamber, wherein the substrate further comprises a channel in fluid communication with the chamber.