Patent ID: 9029166
Filing Date: 2015-05-12
Classification: C07K,G01N

Abstract:
1. A method of identifying a natural substance that is capable of complexation with Ni 2+ , Cu 2+ and/or Fe 2+ , comprising: (a) providing an aqueous, alcoholic or aqueous-alcoholic extract comprising natural substances, (b) providing a device comprising a stationary phase loaded with Ni (c) leading the extract comprising natural substances from (a) through the device from (b), so that the natural substance capable of complexation with Ni (d) optionally rinsing the stationary phase of the IMAC device from (c), loaded with the natural substance capable of complexation with Ni (e) desorption of the natural substance from the stationary phase of the IMAC device from (c), or optionally from (d) is by leading a solution containing one or more desorption agents through the IMAC device from step (c), or optionally from step (d), (f) optionally separating the natural substance from the desorption agent, and (g) identifying the natural substance capable of complexation with Ni