Patent ID: 6194137
Filing Date: 2001-02-27
Classification: A01N,A61K

Abstract:
A method for vitrification of a blood vessel, comprising:immersing the blood vessel in a series of solutions having increasing concentrations of cryoprotectant to achieve a cryoprotectant concentration sufficient for vitrification, each solution of said series of solutions having a temperature above -15.degree. C.;cooling the blood vessel in a solution having said cryoprotectant concentration sufficient for vitrification at an average rate of from 30 to 60.degree. C. per minute from a temperature above -15.degree. C. to a temperature between -80.degree. C. and the glass transition temperature; andfurther cooling the blood vessel at an average rate less than 10.degree. C. per minute from a temperature that is between -80.degree. C. and the glass transition temperature to a temperature below the glass transition temperature to vitrify the blood vessel.