Patent ID: 8932851
Filing Date: 2015-01-13
Classification: C12Q,G01N

Abstract:
1. A kit for enhanced growth and detecting of mycobacteria in a culture medium, said kit comprising: (1) a culture bottle containing a base culture medium; (2) a nutrient supplement comprising fatty acid free BSA, one or more long chain fatty acids, and α-ketoglutarate, an α-ketoglutarate precursor or an α-ketoglutarate derivative selected from the group consisting of glutamate, isocitrate, oxalosuccinate, or mixtures thereof; and (3) an antimicrobial supplement comprises one or more antimicrobial agents for suppressing the growth of contaminating respiratory flora in said culture medium; each separately housed therein; wherein said nutrient supplement and said antimicrobial supplement are added to said culture bottle, and wherein said kit allows for enhanced growth of mycobacteria compared to a kit not containing a supplement comprising fatty acid-free BSA and one or more long chain fatty acids.