Patent ID: 6579686
Filing Date: 2003-06-17
Classification: G01N

Abstract:
A method of screening a compound for its ability to inhibit the binding of clotrimazole to a Constitutive Androstane Receptor (CAR) ligand binding domain (LBD)-containing polypeptide comprising:i) contacting said compound and clotrimazole with said CAR LBD-containing polypeptide under conditions such that clotrimazole can bind to said CAR LBD-containing polypeptide in the absence of said compound, and ii) determining the amount of clotrimazole bound to said CAR LBD-containing polypeptide and comparing that amount to an amount of clotrimazole bound to said CAR LBD-containing polypeptide in the absence of said compound, wherein a reduction in the amount of clotrimazole bound to said CAR LBD-containing polypeptide in the presence of said compound indicates that said compound inhibits the binding of clotrimazole to said CAR LBD-containing polypeptide.