Patent ID: 7834162
Filing Date: 2010-11-16
Classification: C07K

Abstract:
1. A method for purifying Protein A-selected target molecules from a complex solution containing impurities using Protein A-based affinity column chromatography, the method comprising: (a) loading the complex solution onto a Protein A-based affinity chromatography column; (b) adsorbing the Protein A-selected target molecules to a affinity chromatography column matrix within the Protein A affinity chromatography column; (c) washing the impurities from the Protein A affinity chromatography column with an organic solvent-containing solution having a pH of between 6.5 and 8.0 and including between 5% and 60%, by volume, of a mixture of 4-hydroxymethyl-1,3-dioxolane and 5-hydroxy-1,3-dioxane; (d) changing the pH of the organic solvent-containing solution to between 4.0 and 5.0; and (e) eluting the Protein A-selected target molecules from the Protein A affinity chromatography column using the organic solvent-containing solution having a pH of between 4.0 and 5.0.