Patent ID: 6723562
Filing Date: 2004-04-20
Classification: C12C,C12N,C12R

Abstract:
A DNA construct comprising:(i) a selective marker gene, (ii) a galactose-inducible growth inhibition sequence, (iii) a pair of FRT (Flp recombinase recognition target) sequences in the same orientation flanking (i) and (ii), and (iv) a DNA fragment capable of recombining with a yeast chromosomal DNA located at each end of (iii), wherein both of said FRT sequences have deletions of nucleotides in the following sequence: 5â€²-GAAGTTCCTATAC TTTCTAGA GAATAGGMCTTC-3â€² (SEQ ID NO: 1)invertedspacerinvertedrepeat (1)sequencerepeat (2)wherein the FRT sequence which exists at the 5â€² side of the selective marker gene and the GIN sequence has at least 1 but not more than 4 nucleotides deleted from the 5â€² end of the inverted repeat (1) thereof: and wherein the FRT sequence which exists at the 3â€² side of the selective marker gene and the GIN sequence has at least 1 but not more than 5 nucleotides deleted from the 3â€² end of the inverted repeat (2) thereof.