Patent ID: 8067206
Filing Date: 2011-11-29
Classification: C12Q

Abstract:
1. A method for coamplification of two or more target nucleic acids having different sequence compositions present at comparable copy numbers wherein the maximum difference between the lowest and highest copy number is 10-fold said method comprising at least 15 primary amplification cycles, each amplification cycle comprising the sequential steps of: (A) heating a reaction mixture of two or more target nucleic acids, or their primer extension products, at a first temperature, T (B) priming the denatured strands with a set of primers specific to and hybridizable with opposing strands of each target nucleic acid to be amplified, by cooling to a second temperature, T (C) either as a continuation of step (B) or in a separate step, forming primer extension products in a reaction mixture of PCR reagents, by incubation at a third temperature, T (D) in the course of the reaction in each amplification cycle or in an amplification cycle after the last primary amplification cycle detecting one or more of the primer extension products as an indication of one or more of the target nucleic acids.