Patent ID: 8252565
Filing Date: 2012-08-28
Classification: C11B,C12P

Abstract:
1. A method for obtaining a purified diol compound 1-(2 hydroxyethyl)-2,5,5,8a-tetramethyldecahydronaphthalen-2-ol represented by the formula (2): the method comprising: culturing at least one microorganism, adding, prior to or during said culturing, at least one substrate compound represented by the following formula (1a) and/or formula (1b): obtaining a diol compound 1-(2-hydroxyethyl)-2,5,5,8a-tetramethyldecahydronaphthalen-2-ol of the formula (2) in a culture fluid of the cultured microorganism by microbial conversion of the substrate compound filtering the culture fluid containing the diol compound with a filter having an aperture size of 10 to 100 μm; washing a residue on the filter with water or a solvent having an SP value outside the range of 8.3 to 20 (cal/cm dissolving the obtained cake in a solvent having an SP value of 8.3 to 20 (cal/cm filtering or centrifuging the solution, thereby obtaining the purified diol compound.