Patent ID: 6423684
Filing Date: 2002-07-23
Classification: C07K

Abstract:
A method to identify a compound which will modulate the localized enzymatic activity of a first target protein that interacts with a second protein containing at least one WD-40 region which method comprisesdetermining the interaction of said first and second protein containing at least one WD-40 region in the presence and absence of a candidate compound; comparing the level of interaction of said first and second protein in the presence and absence of said candidate compound; and identifying as a successful candidate a compound which modifies the level of interaction of said first and second protein in its presence as opposed to its absence, wherein the first target protein is selected from the group consisting of protein kinase C (PKC) and &bgr; adrenergic receptor kinase (&bgr;ARK) and wherein the WD40 region has the amino acid sequence set forth in SEQ ID NO:69-SEQ ID NO:75, the sequence set forth in SEQ ID NO:28-SEQ ID NO:68 or the sequence set forth in SEQ ID NO:128-SEQ ID NO:134.