Patent ID: 7892854
Filing Date: 2011-02-22
Classification: B01J,C12Q,G01N,Y10T

Abstract:
1. A method of determining the affinity of several different analyte-binding agent interactions, in a reaction environment where several different such interactions between different analytes and binding agents take place, and where some of said analytes are capable of binding to more than one said binding agent at different affinities than where binding to other binding agents, comprising: providing a plurality of particles comprising at least two different particle populations, wherein different particle populations have different binding agents attached thereto, and wherein the particles are associated with a distinguishable chemical or physical characteristic that identifies the binding agents on said particles, and wherein the particles are arranged in a substantially planar array on a substrate; determining the identity of said binding agents by the distinguishable chemical or physical characteristic associated with the particles; contacting the binding agents with an analyte molecule so as to allow the analyte to form an analyte-binding agent complex with one or more binding agents, the formation of said complex resulting in a proportional change in an optical signature associated with the particles whose binding agent is involved in the formation of the complex; wherein said proportional change in optical signature for the analyte-binding agent complexes reflects the affinities characterizing said analyte-binding agent interactions associated with each such proportional change; compiling a matrix of said affinities; and compiling a competitive binding interaction descriptor using a combination of the affinities in the matrix and known concentrations of analyte and binding agents participating in the formation of analyte-binding agent complexes.