Patent ID: 6872530
Filing Date: 2005-03-29
Classification: C12Q

Abstract:
1. A peptide nucleic acid probe-based method for generating data indicative of the presence of a variant in a nucleotide containing compound, comprising: subjecting a peptide nucleic acid probe (PNAP) having fewer than about 30 bases to temperature gradient electrophoresis in the presence of a nucleotide containing compound (NCC), wherein, during temperature gradient electrophoresis, the PNAP and the NCC are subjected to a first temperature and a second, different temperature and, during at least a portion of the temperature gradient electrophoresis, the PNAP and NCC migrate electrophoretically, wherein: irradiating at least the PNAP to generate a spectroscopic signal; and converting the spectroscopic signal into data suitable for determining the presence of the variant in the NCC.