Patent ID: 6274144
Filing Date: 2001-08-14
Classification: A61K,A61P,C08B

Abstract:
A method for producing antibodies specific for a saccharide fragment, said method comprising:a) providing an immunogen comprising a saccharide fragment product conjugated to a carrier, said saccharide fragment product being produced by degrading a larger polysaccharide, the polysaccharide being larger than the saccharide fragment product and said polysaccharide comprising at least one covalent bond between a C1 anomeric carbon of an aldose residue and an oxygen atom of a second residue in a -D or -L glycosidic linkage; said degrading comprising:1) protecting free hydroxyl groups on the larger polysaccharide;2) reacting the larger polysaccharide with ozone to oxidize the C1 anomeric carbon, thus converting the aldose residue to an aldonic acid ester residue; and3) cleaving the aldonic acid ester residue to form the saccharide fragment; andb) administering said immunogen to a suitable host.