Patent ID: 8241915
Filing Date: 2012-08-14
Classification: G01N

Abstract:
1. A method of detecting hemoglobin in a test sample, the method consisting essentially of the steps of: a) adding at least one basic solution to a test sample; b) adding an indicator solution to the test sample to generate a light signal, wherein the indicator solution comprises at least one acridinium compound, wherein steps a) and b) can be performed in any order; and c) measuring the increase in light generated to detect the hemoglobin in the test sample, wherein the light generated is proportional to the amount of hemoglobin in the test sample; and wherein the at least one acridinium compound comprises an acridinium-9-carboxamide or an acridinium-9-carboxylate aryl ester, wherein the acridiniurn-9-carboxamide has a structure according to formula I: wherein R wherein R wherein acridinium-9-carboxylate aryl ester has a structure according to formula II: wherein R wherein R