Patent ID: 6544756
Filing Date: 2003-04-08
Classification: C12N,C12Q,C12R,Y10S

Abstract:
A sorbitol dehydrogenase having the following physicochemical properties:(1) activity: the sorbitol dehydrogenase catalyzes dehydrogenation oxidation of D-sorbitol in the presence of NAD+ to produce D-fructose, and catalyzes a reverse reaction which reduces D-fructose in the presence of NADH to produce D-sorbitol and NAD+; (2) substrate specificity: the sorbitol dehydrogenase exhibits a Vmax/Km value of about 40 or less for galactitol and about 3 or less for L-iditol when the Vmax/Km value for D-sorbitol is taken as 100, and which does not act on D-arabitol, D-mannitol, xylitol and D-glucose; and (3) exhibits a Km value of about 6 mM or less with respect to D-sorbitol.