Patent ID: 8969071
Filing Date: 2015-03-03
Classification: B01L,C12M,G01N,Y10T

Abstract:
1. An apparatus for passive sorting of droplets, comprising: a chip, a main flow channel in said chip, a source of carrier fluid connected to said main flow channel that fills said main flow channel with said carrier fluid and provides a fluid pressure, a source containing a sample and reagents, a sample and reagents channel connected to said source and connected to said main flow channel wherein said sample and said reagents and said sample and reagents channel form droplets of said sample and said reagents and provide a flow stream of said droplets in said main flow channel, an amplification zone in said main flow channel that receives said flow stream of said droplets and provides polymerase chain reaction of said sample in said droplets producing first droplets containing DNA and second droplets that do not contain DNA and are therefore empty wherein said first droplets and said second droplets have substantially the same diameter and wherein said first droplets containing DNA have a first degree of stiffness and wherein said second droplets that are empty have a second degree of stiffness that is less than said first degree of stiffness, a bifurcated flow channel junction connected to said main flow channel in said chip, a first separator flow channel connected to said bifurcated flow channel junction that has a size that provides less fluid pressure and causes said first droplets containing DNA and a first degree of stiffness to flow through said first separator flow channel, and a second separator flow channel connected to said bifurcated flow channel junction that has a size that provides higher pressure and causes said second droplets that are empty and have a second degree of stiffness that is less than said first degree of stiffness to flow through said second separator flow channel thereby providing a separator for separating said second droplets that are empty and have a second degree of stiffness that is less than said first degree of stiffness from said first droplets containing DNA and directing said second droplets into said second separator flow channel.