Patent ID: 8021853
Filing Date: 2011-09-20
Classification: A01K,A61P,C07K,C12N,G01N,Y10S

Abstract:
1. A method for identifying inhibitors of the L-type Ca 2+ channel β 3 protein, comprising: a) contacting recombinant host cells with a calcium indicator that emits detectable signals in the presence of calcium, wherein the recombinant host cells have been transfected with a recombinant expression vector comprising nucleic acid control sequences operatively linked to an L-type Ca b) treating the host cells with one or more test compounds, wherein the treating occurs before, simultaneous with, or after the contacting of the host cells with the calcium indicator; c) stimulating the recombinant host cells with an amount of ATP that is effective to increase intracellular calcium concentration release from cellular stores; and d) detecting signals from the calcium indicator in the recombinant host cells, wherein a test compound-induced increase in the signals from the calcium indicator in the recombinant host cells as compared to a control indicates that the test compound promotes release of calcium from intracellular stores, and wherein the test compound is an inhibitor of the L-type Ca