Patent ID: 6872817
Filing Date: 2005-03-29
Classification: C12Q

Abstract:
1. A method of staining by in situ hybridization a target chromosome, over the entire length of the body of the target chromosome, the method comprising: providing a heterogeneous mixture comprising labeled unique sequence nucleic acid fragments which are substantially complementary to nucleic acid segments substantially uniformly distributed over the entire length of the body of the target chromosome, and which have been prepared from chromosome-specific DNA which has been isolated from a pre-selected chromosome, wherein the pre-selected chromosome has first been isolated away from other chromosomes; and employing the heterogeneous mixture and the target chromosome in in situ hybridization so that hybridization of unique segments within the labeled nucleic acid to the target chromosome is allowed and the presence of fluorescently labeled unique sequence nucleic acid fragments over substantially the entire length of the body of the target chromosome is detected, wherein the target chromosome is present as a morphologically identifiable chromosome during the in situ hybridization, and wherein substantially all of the repetitive sequences within the target chromosome are not stained by in situ hybridization.