Patent ID: 8119782
Filing Date: 2012-02-21
Classification: C12Q

Abstract:
1. A composition comprising: (i) (a) a first plurality of deoxyribozymes, each deoxyribozyme of which cleaves a first substrate when contacted with a first oligonucleotide of a plurality of oligonucleotides but not when contacted with any one of the remainder of the plurality of oligonucleotides; (ii) a fourth plurality of deoxyribozymes, each deoxyribozyme of which cleaves a second substrate when contacted with a first oligonucleotide of a plurality of oligonucleotides, wherein the first substrate comprises a first fluorophore and the second substrate comprises a second fluorophore, and wherein cleavage of the first substrate causes an increase in fluorescence of the first fluorophore and cleavage of the second substrate causes an increase in fluorescence of the second fluorophore, and wherein the first and second fluorophores have different emission spectra, and wherein the first, second, third and fourth pluralities of deoxyribozymes are distributed in a plurality of compartments such that each compartment contains (i) the first substrate, (ii) the second substrate, (iii) at least two deoxyribozymes, each of which is, independently, of the first, second, third or fourth plurality of deoxyribozymes.