Patent ID: 6805869
Filing Date: 2004-10-19
Classification: A61K,C07K,C12N

Abstract:
A method of preparing an immunogenic composition, comprising the steps of:(a) providing an autologous target tumor or pathogen infected cell; (b) treating the tumor or pathogen infected cell with IFN-&ggr; and/or TNF-&agr; to increase concentration of a primary T cell activation molecule or a costimulatory T cell activation molecule in the target tumor or pathogen infected cell; (c) providing a bispecific monoclonal antibody including one or more binding sites for one or more costimulatory molecules on a surface of one or more T cells of a patient mammal, wherein the bispecific antibody is a CD28:gp55 bispecific monoclonal antibody; (d) attaching the bispecific monoclonal antibody to the target tumor or pathogen infected cell; and (e) collecting a pharmaceutically effective amount of the target tumor or pathogen infected cell with the attached bispecific monoclonal antibody.