Patent ID: 6723514
Filing Date: 2004-04-20
Classification: C12N,C12Q

Abstract:
A method for releasing, separating and separately visualizing the presence of extrachromosomal DNA and genomic DNA in a microorganism said method comprising:(a) suspending said microorganism in a first solution that causes lysis of said microorganism and the liberation of said extrachromocomal DNA and said genomic DNA from said microorganism so as to provide a first suspension; (b) optionally heating said first suspension for at least 10 seconds at least 65Â° C.; (c) combining said first suspension with a second solution comprising a restriction endonuclease so as to provide a second suspension, wherein said extrachromosomal DNA and said genomic DNA are digested, released and separately detectable from each other; (d) detecting the presence of said extrachromosomal DNA and said genomic DNA in said second suspension, without prior separation of said extrachromosomal DNA and said genomic DNA from other cellular components.