Patent ID: 8398838
Filing Date: 2013-03-19
Classification: G01N

Abstract:
1. A method of gradient gel electrophoresis shaping, comprising: Causing a first kind of at least two liquid basic materials to be continuously input into a collecting trough of a seat, wherein a roller is at least partially disposed within the collecting trough, the liquid basic materials suitable for generating a gradient gel; Causing a second kind of said basic liquid materials to be input into said collecting trough; Causing a mixing ratio of said second kind of liquid basic materials to said first kind of liquid basic materials to increase as the liquid basic materials are input into the collecting trough; Causing said roller to rotate; and Outputting said liquid basic materials from said collecting trough into a receiving space within a carrier sheet set that is held in static relationship with said seat, thereby continuously outputting liquid gel into said statically held carrier sheet set that forms a gradient by virtue of said increasing mixing ratio, wherein said carrier sheet set includes a front carrier sheet, a rear carrier sheet and at least two spacing sheets between said front and said rear carrier sheets, so that said front and rear carrier sheets form therebetween said receiving space; and Said positioning member includes a positioning frame fixed on said seat, two lateral pressing plates pivotally provided at two lateral sides of and in front of said positioning frame, A front of said positioning frame is provided with a positioning trough for accepting said carrier sheet set, a bottom of said positioning trough provided with an elongate hole in fluidic connection with said gel output port; Lateral sides of a front wall of the positioning trough each including an upright height adjusting groove with a height control plate disposed therein, each height adjusting groove including an upper and lower recess, and each height control plate has a positioning protrusion configured to selectively engage with the corresponding upper and lower recesses; Each height control plate has thereon at least two protruding pieces respectively provided on a top and a bottom end thereof configured to engage with said rear carrier sheet; and A top of a front of said positioning trough further including at least two pressing camps in contact with an upper edge of said rear carrier sheet.