Patent ID: 7741073
Filing Date: 2010-06-22
Classification: C12N

Abstract:
1. An isolated polynucleotide encoding an intraplastid-targeting polypeptide, wherein the polypeptide consists of: domain A consisting of: i) amino acids 60-100 of SEQ ID NO: 1; or ii) a fragment of a protein of an inner membrane of a chloroplast envelope, said protein being recognized by an antibody directed against SEQ ID NO: 1, and said fragment having at least 70% identity with amino acids 60-100 of SEQ ID NO: 1 or SEQ ID NO: 3 and when combined with domain B has plastid targeting activity; and a domain B located at the N-terminal end of domain A consisting of iii) a fragment of amino acids 1-59 of SEQ ID NO: 1, said fragment including at least amino acids 49-59 of SEQ ID NO: 1 or SEQ ID NO: 3, or iv) a fragment of a protein of an inner membrane of a chloroplast envelope, said protein being recognized by antibodies directed against the polypeptide SEQ ID NO: 1, and said fragment having at least 60% identity with said fragment iii) and when combined with domain A has plastid targeting activity.