Patent ID: 8969073
Filing Date: 2015-03-03
Classification: A61M,G01N

Abstract:
1. An appliance for the isolation of target cells from blood with the objective of an application for the treatment of human diseases, for animals, in bio-technology and medicinal diagnostics, comprising: a reaction vessel of biocompatible material, in which blood is situated, wherein the blood comprises target cells, functionalised micro-particles having a diameter greater than 10 μm and less than 800 μm, which recognise and bind target cells, the micro-particles having the same or different fractional sizes, said micro-particles are freely mobile and able to be guided from the reaction vessel to a particle separation system, a particle separation system based on two or more membrane/screen/sieves forming a sieve cascade with pores having a size greater than 10 μm, less than 800 μm, and less than the diameter of the smallest fraction of functionalised micro-particles, which allow the cells, but not the micro-particles to pass, for separation of a medium with a cell mixture and micro-particles by hydrostatic pressure into a cell mixture with free micro-particles not bound to cells beyond the membrane and specific complexes from functionalized smallest fraction of microparticles and target cells into a medium on the opposite side of the membrane, only containing the specific complexes from functionalised micro-particles and larger than the smallest fraction of target cells, a screen/hollow-fibre membrane, the latter equipped with a pore size merely permitting the passage of the cell mixture without micro-particles, a pump in order to pump/guide fluid into the reaction vessel via a valve, a valve, via which the fluid is pumped/guided into the reaction vessel, a valve, via which the cell mixture without micro-particles is guided off from the particle separation system, a hose system for (A) guiding or pumping of fluid with a cell mixture into a reaction vessel containing functionalised micro-particles via a valve and (b) guiding the medium with cell mixture and functionalised micro-particles from the reaction vessel via a screen/sieve or with a hollow-fibre membrane for separation of the medium into a cell mixture without functionalised micro-particles and into a cell mixture with functionalised micro-particles or to guide the medium with cell mixture and functionalised micro-particles from the reaction vessel via a screen/sieve for separation of the medium into a cell mixture and specific complexes from functionalized smaller-fraction micro-particles and target cells and with free micro-particles not bound to cells beyond the membrane and into a medium on this side of the membrane which only contains the specific complexes from functionalised micro-particles and target cells and (C) to guide off the separated cell mixtures without functionalised micro-particles via a valve and (D) to guide the separated cell mixtures with the functionalised micro-particles and the cells bound to it back to the reaction vessel or to guide off the cell mixture with the functionalised micro-particles and the cells bound to it for analytical or preparative purposes, and, for continuous use, various hose systems, membranes, pumps and valves, in order to be able to carry out all the actions of isolation, separation, processing and removal of the cells.