Patent ID: 6623982
Filing Date: 2003-09-23
Classification: G01N,Y10S,Y10T

Abstract:
A method for isolating a target bioentity from a biological sample by means of colloidal magnetic particles, comprising:a) obtaining a biological sample suspected of containing said target bioentity together with non-target bioentities and endogenous aggregating factors; b) contacting said biological sample with a reagent effective to inactivate any endogenous aggregating factors present in said sample without adversely affecting cellular morphology; c) preparing an immunomagnetic suspension comprising a mixture of said sample and colloidal, magnetic particles coupled to a biospecific ligand having binding affinity for at least one characteristic determinant present on said target bioentity; and d) subjecting said immunomagnetic suspension to a magnetic field to obtain a target bioentity-enriched fraction.