Patent ID: 8956867
Filing Date: 2015-02-17
Classification: C07K,C12N

Abstract:
1. A method for culturing human pluripotent stem cells to obtain a contracting embryoid body in which at least 50% of cells of the contracting embryoid body express MF20 or MLC2a, the method comprising the steps of: harvesting aggregates or colonies of substantially undifferentiated human pluripotent stem cells from a microwell that constrains the growth of the human pluripotent stem cells in three dimensions and having a lateral dimension of about 100 microns; differentiating the harvested aggregates or colonies of pluripotent stem cells under unconstrained culture conditions for a period sufficient to form a population of embryoid bodies comprising a plurality of contracting embryoid bodies, wherein at least 50% of the cells of each contracting embryoid body of the plurality express MF20 or MLC2a.