Patent ID: 8563309
Filing Date: 2013-10-22
Classification: C12N

Abstract:
1. A method of producing a cell mass capable of serving as a primitive organ-like structure comprised of combinations of keratinocytes and hair papilla cells, comprising: preparing cultures containing keratinocytes and hair papilla cells; mixing said keratinocytes and hair papilla cell cultures followed by adding a Wnt signal activator demonstrating an inhibitory activity on glycogen synthase-kinase-3 (GSK-3) to the mixed cell culture; subjecting the culture containing the Wnt signal activator to hanging drop method or spheroid formation method over a predetermined time period; and replacing the medium of the culture cultured by the hanging drop method or spheroid formation method with medium not containing Wnt signal activator and further culturing for a predetermined time period; wherein, at least one type of the keratinocytes and the hair papilla cells is maintained in an undifferentiated state.