Patent ID: 6627416
Filing Date: 2003-09-30
Classification: C12Q

Abstract:
A process for detecting nucleic acids in a sample, the process comprisinga) synthesizing by a nucleic acid amplification reaction multiple copies of a double-stranded nucleic acid from a single-stranded nucleic acid in the sample by the enzymatic incorporation in the single-stranded nucleic acid of a compound of formula (I) â€ƒwherein: B is a nucleobase, W and Z is each independently, OR1, SR1, N(R1)2 or R1, wherein R1 is hydrogen or a lipophilic radical, X is OR2, SR2 or B(R2)3, wherein R2 is hydrogen, a cation or a lipophilic radical, Y is NR3 or S, wherein R3 represents hydrogen or a lipophilic radical, and R is hydrogen, a cation or a lipophilic radical, b) subjecting the double-stranded nucleic acid comprising at least one nucleobase of a compound of formula I to site-directed cleavage to obtain nucleic acid fragments comprising a 5â€² modified end by the site-directed cleavage reaction, and c) detecting the nucleic acid fragments of step b).