Patent ID: 6033823
Filing Date: 2000-03-07
Classification: C12N,C12P

Abstract:
An isolated mutant prokaryotic Penicillin G acylase or its preenzmye or preproenzyme having:an amino acid substitution at one or more of the positions corresponding to A139 to A142 and A148 to A152 as set forth in SEQ ID NO: 27, B20 to B27, B31, B49 to B52, B56, B57, B65, B67 to B72, B154 to B157, B173 to B179, B239 to B241, B250 to B263, B379 to B387, B390, B455, B474 to B480 as set forth in SEQ ID NO:32 in Alcaligenes faecalis Penicillin G acylase or its pre- or preproenzyme, wherein the substitution at position A141 results in a substituent amino acid selected from the group consisting of Ala, Arg, Asn, Cys, Gln, Glu, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, and Val; andan altered substrate specificity or altered specific activity relative to the corresponding wild-type unsubstituted Penicillin G acylase.