Patent ID: 6518053
Filing Date: 2003-02-11
Classification: C12N

Abstract:
An isolated nucleotide sequence encoding a modified esterase having thermostable esterase activity, which is obtained by modifying the amino acid sequence shown by SEQ ID NO:2 and where the modification is selected from any one of the following:(1) an amino acid substitution where the 325th amino acid in the amino acid sequence shown by SEQ ID No:2 is substituted with isoleucine; (2) an amino acid substitution where the 240th amino acid in the amino acid sequence shown by SEQ ID No:2 is substituted with alanine, and 288th amino acid is substituted with alanine; and (3) an amino acid substitution where 43rd amino acid sequence shown by SEQ ID No:2 is substituted with serine.