Patent ID: 6160104
Filing Date: 2000-12-12
Classification: C12Q

Abstract:
A method for detecting a toxicological effect of a test compound associated with increased or decreased levels of a polynucleotide sequence in a sample comprising:a) treating a sample with a toxin;b) obtaining a polynucleotide sequence from the sample treated with the toxin;c) contacting the polynucleotide sequence with an array comprising a combination of polynucleotides comprising SEQ ID NOs. 1-56 under conditions effective to form one or more hybridization complexes;d) detecting the hybridization complexes, wherein the presence, absence or change in amount of the hybridization complex, as compared with the hybridization complexes formed by SEQ ID NOs:1-56 with polynucleotide sequence from an untreated sample, is indicative of a toxicological response to the toxin; thene) measuring the level of polynucleotide sequences in a sample treated with a test compound using the method of steps (c) and (d); andf) comparing the level detected in step (e) with the level detected in step (c) wherein an increase or decrease in the level detected in step (e) indicates a toxicological response to the test compound.