Patent ID: 7723078
Filing Date: 2010-05-25
Classification: C12Q

Abstract:
1. A method for the transcription based amplification of a double stranded target HBV nucleic acid sequence starting from HBV DNA optionally present in a sample, said method comprising: a) simultaneously incubating the sample, suspected to contain HBV, in an amplification buffer, in the absence of a restriction primer, with b) maintaining the thus created reaction mixture of (a) under the appropriate conditions for a sufficient amount of time for a digestion by the restriction enzyme to take place; c) subjecting the reaction mixture of (b) to a heat treatment at a temperature and time sufficient to inactivate the restriction enzyme and/or to render at least partially a double strand single stranded; d) adding the following reagents to the reaction mixture of (c): e) maintaining the reaction mixture of (d) under the appropriate conditions for a sufficient amount of time for the amplification to take place.