Patent ID: 9187782
Filing Date: 2015-11-17
Classification: C12Q

Abstract:
1. A method for characterizing by length of a repetitive sequence in a sample nucleic acid, said repetitive sequence having a location between a 5′ flanking region and a 3′ flanking region, the method comprising the steps of: (a) hybridizing a polymerization primer to said 3′ flanking region; (b) hybridizing a modified oligonucleotide to said 5′ flanking region, said modified oligonucleotide being resistant to displacement by a polymerase enzyme; (c) extending the polymerization primer in a 5′ to 3′ direction by using said polymerase enzyme, said extending stopping at 5′ end of the modified oligonucleotide; and (d) said extending comprising the step of adding nucleotides complementary to the sample sequence and the step of adding a dideoxy nucleotide selected to be only complementary to a nucleotide in the 5′ flanking region, whereby extending proceeds from said polymerization primer to the modified oligonucleotide unless terminated by an added dideoxynucleotide, wherein the repetitive sequence is characterized by length of extending in step (c) and (d).