Patent ID: 6365363
Filing Date: 2002-04-02
Classification: C12Q

Abstract:
A method of determining cholesterol present on skin surface, comprising the steps of:a. providing an open-ended vessel having a base; b. providing an enzyme solution comprising 2.0 to 2.5 units of cholesterol oxidase, 0.04 to 0.06 weight % of sodium dezoxycholate, and 0.1 to 0.2 weight % of 3-dodecyl-dimethyl-ammonium-propanesulfonate, buffered to a pH of 6.8 in water; c. bringing said vessel into contac with the skin surface in a manner such that the base of said vessel sealingly engages the skin surface, thereby delimiting an area of said skin surface within said vessel; d. adding a predetermined amount of said enzyme solution into said vessel so that said enzyme solution contacts the skin surface over the delimited area thereof; e. allowing said enzyme solution to remain in contact with the skin surface over the delimited area thereof for a period of time sufficient for the cholesterol oxidase to catalyze oxidation of cholesterol and cause formation of hydrogen peroxide; and, f. measuring the amount of hydrogen peroxide formed in step (e), the amount of hydrogen peroxide measured being indicative of the concentration of skin cholesterol.