Patent ID: 7759092
Filing Date: 2010-07-20
Classification: C07K,C12P

Abstract:
1. A method of producing an IL-29 polypeptide comprising: (a) culturing an ompT deficient and fhuA deficient in a first growth medium under conditions wherein the encoded IL-29 polypeptide is expressed in a shake flask to an OD600 of 5 to 20; (b) inoculating a fermentation vessel with 1 to 5% v/v of shake flask medium containing host cells; (c) culturing the host cells in a second growth medium at a pH of 6.2 to 7.2, wherein a carbohydrate feed solution is fed into the fermentation vessel at 6 to 8 hours elapsed fermentation time; (d) adding an inducing agent to the fermentation vessel at 20 to 30 hours elapsed fermentation time; and (e) harvesting the host cells at 48 to 56 hours elapsed fermentation time.