Patent ID: 7399610
Filing Date: 2008-07-15
Classification: C12P

Abstract:
1. A method for cell-free protein synthesis, said method comprising: contacting a reaction solution comprising an insect cell extract solution and necessary components for protein synthesis with an external solution through a semipermeable membrane, wherein the reaction solution is subjected to translation to produce a protein, and maintaining the production of said protein, while (a) supplying exogenous mRNA and (b) removing components from said reaction solution by passing said components through the semipermeable membrane into said external solution, said components comprising substances which inhibit protein synthesis and degradation products which form during protein synthesis, wherein the insect cell extract solution is obtained by separating the insect cell extract from an insect cell, said insect cell extract comprising insect cell components essential for protein synthesis, wherein the insect cell extract solution is an established culture cell derived from insects of Lepidoptera, Orthoptera, Diptera, Hymenoptera, Coleoptra, Neuroptra, Hemiptera, wherein said components removed from the reaction solution comprise one or more compounds selected from the group consisting of adenosine diphosphate (ADP), adenosine monophosphate (AMP), gunanosine 5′-diphosphate (GDP), guanosine 5′-monophosphate (GMP), phosphates, pyrophosphates, and products which are degraded and formed during protein synthesis and wherein the necessary components for protein synthesis comprise one or more compounds selected from the group consisting of potassium salt, magnesium salt, dithiothreitol, adenosine triphosphate, guanosine triphosphate, creatine phosphate, creatine kinase, amino acid, RNase inhibitor, tRNA, exogenous mRNA, buffer, and EGTA.