Patent ID: 8021850
Filing Date: 2011-09-20
Classification: G01N

Abstract:
1. A sandwich-ELISA equivalent immunoassay for detecting and quantifying an analyte in a fluid sample without sandwich-ELISA's strict requirement of a pair matched antibodies, the universal tandem solid-phases based immunoassay (UTSIA), which comprises: (a) an affinity binding based specific analyte capture process to immobilize the analyte in the fluid sample on a solid phase via contacting the fluid sample with an affinity binding solid phase; (b) a dissociation process to release the analyte immobilized on the affinity binding solid phase into a liquid phase via incubating the analyte immobilized on the affinity binding solid phase with a dissociation buffer; (c) a non-affinity physical absorption process to coat the analyte released in the liquid phase on a solid phase via incubating the analyte released in the liquid phase with a non-affinity binding solid phase; (d) an affinity binding based specific analyte detection process to detect the analyte via incubating the analyte coated on the non-affinity binding solid phase with a labeled detection molecule to form a complex of the analyte and the labeled detection molecule on the solid phase, or via incubating the analyte coated on the non-affinity binding solid phase with an unlabeled detection molecule and a labeled molecule to form a complex of the analyte, the unlabeled detection molecule, and the labeled molecule on the solid phase; and (e) a signal measurement process to detect the amount of the label in the complex in the presence of the solid phase in step (c) that immobilized the complex.