Patent ID: 7638297
Filing Date: 2009-12-29
Classification: C12N,C12P

Abstract:
1. A translation system comprising: an orthogonal lysyl tRNA (lysyl O-tRNA); an orthogonal aminoacyl tRNA synthetase (O-RS) that preferentially charges the orthogonal lysyl tRNA with homoglutamine; or the orthogonal lysyl tRNA (lysyl O-tRNA) and the orthogonal aminoacyl tRNA synthetase (O-RS); wherein the O-RS is PhΔAD (SEQ ID NO: 28), or an O-RS comprising at least 95% identity to PhΔAD (SEQ ID NO: 28) further comprising an isoleucine at a position corresponding to position 41 and a serine at a position corresponding to position 268; and wherein the lysyl O-tRNA comprises an anti-codon loop comprising a CU(X) wherein the O-RS in combination with the O-tRNA of SEQ ID NO: 26 and homoglutamine is at least 50% as effective at suppressing a selector codon as an I41 and S268 mutant of PhΔAD (SEQ ID NO: 28; a mutant of