Patent ID: 6165335
Filing Date: 2000-12-26
Classification: B01J,C12Q,C40B,G01N,Y10S

Abstract:
A biosensor apparatus for detecting a binding event between a ligand and ligand-binding agent, comprisingmeans defining a biosensor surface,carried on said biosensor surface, two-subunit heterodimer complexes composed of first and second peptides that together form an .alpha.-helical coiled-coil heterodimer, where said first peptide is attached to the biosensor surface,a ligand covalently attached to the second peptide in the two-subunit heterodimer complexes, accessible for binding by a ligand-binding agent,means for introducing onto the biosensor surface, an analyte selected from the group consisting of a ligand-binding agent, and a ligand or ligand analog capable of competing with a ligand-binding agent for binding to said ligand, said apparatus also including a ligand-binding agent when the analyte is a ligand or ligand analog, andmeans for detecting the binding of a ligand-binding agent to the ligand on the biosensor surface.