Patent ID: 6730507
Filing Date: 2004-05-04
Classification: A01K,A61K,C12N

Abstract:
An adenoviral vector gene delivery system comprising:(a) a helper dependent adenovirus vector, hdAd, comprising a genome lacking virion capsid protein coding sequences, hut encoding a gene and expression control sequences, the expression of which is desired in a recipient cell; (b) helper adenoviruses of different serotypes, each of which encodes proteins whose functions are required for, when introduced into a cell line, replication and packaging of said hdAd genome, and wherein each said helper adenovirus comprises a packaging signal flanked on either side by at least one lox site; and (c) said cell line, wherein Cre recombinase is expressed in said cell line, into which may be introduced said hdAd and first one of said helper adenoviruses having a first particular serotype, such that packaged hdAd having the first particular serotype results, and wherein introduction of said hdAd and a second one of said helper viruses having a second particular serotype into cells of said cell line results in a packaged hdAd having the second particular serotype.