Patent ID: 8865398
Filing Date: 2014-10-21
Classification: C07K,G01N

Abstract:
1. A method for detection of hepatitis C virus core protein and antibodies to hepatitis C virus core protein in a sample comprising the steps of: a) contacting said sample with a first capture peptide consisting of SEQ ID NO:4, a second capture peptide consisting of SEQ ID NO:3 or SEQ ID NO:54, and a third capture peptide consisting of SEQ ID NO:5 under conditions that allow formation of capture peptide:antibody complexes between said first, second and third capture peptides and said antibodies to hepatitis C virus core protein b) contacting said sample with a first antibody under conditions that allow formation of an antibody:antigen complex between said first antibody and said hepatitis C virus core protein, wherein said first antibody specifically binds said hepatitis C virus core protein at a first epitope comprising SEQ ID NO:6; c) detecting any capture peptide:antibody complexes formed in step (a) as a measure of said antibodies to said hepatitis C virus core protein, wherein said antibodies may be detected in the seroconversion and chronic phases of infection; and d) detecting any antibody:antigen complexes formed in step (b) as a measure of said hepatitis C virus protein.