Patent ID: 6218183
Filing Date: 2001-04-17
Classification: A01H,A01N,C12Q

Abstract:
A screening method for the identification of a plant which has antimicrobial activity comprising the following steps in any order:a) isolating a plant species growing naturally and surviving on fresh cattle dung or cattle dung which has been decomposing for up to 15 days;b) identifying the isolated plant species;c) collecting a seed from the identified plant species;d) sterilizing the collected seed;e) incubating the sterilized seed with a test plate containing (i) a bacterial strain for about 24 hours at 37.degree. C. or (ii) a fungal strain for about 42 hrs at 28.degree. C.;f) determining the growth of (i) the bacterial strain or the growth of (ii) the fungal strain in the presence of the seed, wherein a decrease in growth of (i) the bacterial strain or (ii) the fungal strain, is indicative that the seed has antimicrobial activity.