Patent ID: 8927214
Filing Date: 2015-01-06
Classification: C07K,C12N,C12Q

Abstract:
1. A method of isolating nucleic acid and undenatured and unreduced protein from the same biological sample, comprising, in the following order: a) disrupting the biological sample, which comprises protein that is not denatured and not reduced; b) contacting the disrupted biological sample of (a) with a protein lysis buffer that lacks any component that denatures or reduces protein to produce a first lysate; c) centrifuging the first lysate of (b) to produce a first supernatant containing undenatured and unreduced protein and a pellet containing nucleic acid; d) removing the first supernatant of (c), thereby isolating undenatured and unreduced protein from the biological sample; e) contacting the pellet of (d) with nucleic acid lysis buffer to produce a second lysate; f) centrifuging the second lysate of (e) to produce a second supernatant containing nucleic acid; and g) removing the second supernatant of (f), thereby isolating nucleic acid from the same biological sample.