Patent ID: 8137934
Filing Date: 2012-03-20
Classification: A61K,A61P,C07K,G01N

Abstract:
1. A method for inducing differentiation into a retinal photoreceptor cell and suppressing differentiation into a bipolar cell, an amacrine cell and a Muller glia cell, which method comprises: introducing a DNA or an RNA encoding an Otx2 protein into an eye ball tissue-derived cell, an embryonic stem cell, a neural stem cell or a neural precursor cell; and culturing the resulting cell containing the DNA or RNA encoding the Otx2 protein to express an Otx2 protein, or increase an amount of expression of an Otx2 protein, wherein the Otx2 protein is (1) a protein having the amino acid sequence represented by SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 5; or (2) a protein having (i) an amino acid sequence in which 1 or 2 amino acid(s) in the amino acid sequence represented by SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 5 is/are deleted, (ii) an amino acid sequence in which 1 or 2 amino acid(s) is/are added to the amino acid sequence represented by SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 5, (iii) an amino acid sequence in which 1 or 2 amino acid(s) in the amino acid sequence represented by SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 5 is/are substituted with other amino acid(s), or (iv) an amino acid sequence as a combination thereof, the protein having amino acid sequence (i), (ii), (iii) or (iv) having an action of inducing differentiation into a retinal photoreceptor cell and suppressing differentiation into a bipolar cell, an amacrine cell and a Muller glia cell, wherein said action is equivalent to that of the protein having the amino acid sequence represented by SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 5.