Patent ID: 9051601
Filing Date: 2015-06-09
Classification: C07H,C12N,C12Q

Abstract:
1. A method for isolating nucleic acid from a sample, comprising the steps of: a. mixing a sample containing nucleic acids with a non-specific capture probe comprising a first region that is a randomized poly(k) sequence at a length of 18 residues comprising G and T nucleotides or G and U nucleotides attached to a second region that is a first specific binding partner (SBP); b. incubating a reaction mixture containing a support and the mixture of nucleic acids and non-specific capture probe in conditions that allow hybridization of the first region with all or a portion of the nucleic acids in the sample and that allow for hybridization of the SBP with a second specific binding partner (SBP′) immobilized to the support, thereby forming a hybridization complex; and c. separating the support from the solution phase of the reaction mixture, thereby isolating the nucleic acids away from other components in the sample.