Patent ID: 7521180
Filing Date: 2009-04-21
Classification: C12Q

Abstract:
1. A method for evaluating microsatellite instability associated with a tumor selected from the group consisting of colon, gastric, and endometrial tumors, which comprises the steps of: a) amplifying microsatellite loci in a biological sample comprising genomic DNA from said tumor with primers; b) determining a size of the DNA amplification product for each locus; and c) comparing the size of said DNA amplification product with a size of a amplification product obtained from normal DNA with identical primers; and wherein at least one microsatellite locus selected from the group consisting of NR 21, NR 22 and NR 24, is amplified, and wherein microsatellite instability at said locus is indicated if the size of the amplification product obtained from the tumoral DNA is shorter by more than 2 bp than the size of the amplification product obtained from the normal DNA.