Patent ID: 7381805
Filing Date: 2008-06-03
Classification: A61K,A61P,C07K,C12P

Abstract:
1. A composition comprising a mixture of positional PEG isomers of a PEGylated G-CSF polypeptide, wherein the polypeptide comprises an amino acid sequence consisting of SEQ ID NO:1 with the substitutions K16R, K34R, K40R, T105K and S159K, and wherein at least about 80% of the mixture of positional PEG isomers consists of two positional PEG isomers each consisting of three attached PEG moieties, wherein one of the two isomers has PEG moieties attached at the N-terminal, Lys23 and Lys159 and the other of the two isomers has PEG moieties attached at the N-terminal, Lys105 and Lys159, and wherein the attached PEG moieties are about 5 kDa molecular weight.