Patent ID: 8637280
Filing Date: 2014-01-28
Classification: C07K,C12P

Abstract:
1. A genetically modified TS08 polynucleotide selected from the group consisting of: (a) polynucleotides encoding a TS08 polypeptide active in the transport of substances over a membrane and having manganese resistance protein 1 activity (TS08 polypeptide) comprising an amino acid sequence according to SEQ ID NO: 2; (b) a polynucleotide comprising a nucleotide sequence according to SEQ ID NO: 1; (c) polynucleotides comprising a nucleotide sequence obtainable by nucleic acid amplification such as polymerase chain reaction, using genomic DNA from a microorganism as a template and a primer set having the nucleotide sequences of SEQ ID NO:3 and SEQ ID NO:4, respectively; (d) polynucleotides comprising a nucleotide sequence encoding a fragment or derivative of a polypeptide encoded by a polynucleotide of any of (a) to (c) wherein in said derivative one or more amino acid residues are conservatively substituted compared to said polypeptide, and said fragment or derivative has the ability to increase citric acid production; (e) polynucleotides which are at least 95% identical to a polynucleotide as defined in any one of (a) to (d) and which encode a TS08 polypeptide, or the full complementary strand of said polynucleotide and which retain the ability to increase citric acid production; and (f) fragments of the polynucleotides of (e) which retain the ability to increase citric acid production.