Patent ID: 7897346
Filing Date: 2011-03-01
Classification: A61K,A61P,C07K,Y10T

Abstract:
1. A method for detecting a nucleic acid of interest, said method comprising providing a PNA derivative of formula I, which is detectably labeled, wherein q is 0; D′ is, independently of each other, hydroxyl, mercapto, amino, alkylamino, or acylamino; V is oxygen, sulfur, or NR V′ is, independently of any other V′, oxygen, sulfur, NR U is, independently of any other U, oxygen, sulfur, or NH; u′ is, independently of any other u′, from 1 to 10; W and W′ are, independently of each other, oxygen, sulfur, or NR Y and Y′ are, independently of each other, hydroxyl, mercapto, oxyanion, thioate, or NR X and X′ are, independently of each other, Z and Z′ are, independently of each other, R R n is from 0 to 10; m is from 0 to 10; and wherein {POLY} is described by Formula II wherein {BLOCK} is, independently of any other {BLOCK}, a group selected from Formula IIIA, Formula IIIB, and Formulae IV A to IV G, wherein each {BLOCK} building block can be different, and wherein z″ is from 0 to 100; G is (CR A is, independently of any other A, a group (CR B is, independently of any other B, either an aromatic radical, a heteroaromatic radical, hydrogen, hydroxyl, or C D is, independently of any other D, a group (CR E is, independently of any other E, a group (CR wherein R and physiologically tolerated salts of the PNA derivative of Formula I, with the provisos that at least one Y, Y′, Z, or Z′ radical is hydroxyl, mercapto, oxyanion, or thioate, and that at least one B radical is a nucleobase; wherein the detectably labeled PNA derivative comprises a base sequence that hybridizes with at least one sequence present in the nucleic acid of interest under selected conditions; wherein specific binding indicates the presence of the nucleic acid of interest in the sample.