Patent ID: 7504365
Filing Date: 2009-03-17
Classification: G01N

Abstract:
1. A method of making an array, the method comprising: applying building block molecules to a solid support in a plurality of regions, each region: comprising 2, 3, 4, 5, or 6 different building block molecules; being a contiguous portion of the surface of the solid support with the different building block molecules distributed randomly throughout the contiguous region; and having the shape of a spot; independently covalently coupling the different building block molecules to the solid support in the regions; producing an array comprising a candidate artificial receptor, a lead artificial receptor, a working artificial receptor, or a combination thereof, in which a plurality of adjacent building block molecules form a binding site; wherein: A) a first region comprises a first combination of building block molecules and a second region comprises a second combination of building block molecules; B) one or more of the building block molecules comprises a tether, a framework and n recognition elements and is independently of the formula: in which: n=1, 2, or 3; each recognition element is independently covalently coupled to the framework; the tether is covalently coupled to the framework; and the tether comprises a functional group effective for covalent coupling to a support or a linker; the framework is alkyl, substituted alkyl, cycloalkyl, heterocyclic, substituted heterocyclic, aryl alkyl, aryl, heteroaryl, or heteroaryl alkyl; substituted with 1 to 4 functional groups; the functional groups independently being carboxyl, amine, hydroxyl, phenol, carbonyl, or thiol; each recognition element is independently a 1-12 carbon alkyl, substituted alkyl, cycloalkyl, heterocyclic, substituted heterocyclic, aryl alkyl, aryl, heteroaryl, or heteroaryl alkyl moiety; substituted with a group with a property of positive charge, negative charge, acid, base, electron acceptor, electron donor, hydrogen bond donor, hydrogen bond acceptor, free electron pair, π electrons, charge polarization, hydrophilicity, or hydrophobicity; the tether is a polyethylene glycol, a polyamide, a linear polymer, an oligosaccharide, or a polysaccharide; and when the building block molecule comprises an amino acid derivative it comprises a single derivatized amino acid and the framework is the amino acid derivative; and C) one or more of the building block molecules comprises a framework and n recognition elements and is independently of the formula: in which: n=1, 2, or 3; each recognition element is independently covalently coupled to the framework; and the framework comprises a functional group effective for covalent coupling to a support or a linker; the framework is alkyl, substituted alkyl, cycloalkyl, heterocyclic, substituted heterocyclic, aryl alkyl, aryl, heteroaryl, or heteroaryl alkyl; substituted with 1 to 4 functional groups; the functional groups independently being carboxyl, amine, hydroxyl, phenol, carbonyl, or thiol; each recognition element is independently a 1-12 carbon alkyl, substituted alkyl, cycloalkyl, heterocyclic, substituted heterocyclic, aryl alkyl, aryl, heteroaryl, or heteroaryl alkyl moiety; substituted with a group with a property of positive charge, negative charge, acid, base, electron acceptor, electron donor, hydrogen bond donor, hydrogen bond acceptor, free electron pair, π electrons, charge polarization, hydrophilicity, or hydrophobicity; and when the building block molecule comprises an amino acid derivative, wherein amino acid derivatives are defined as a core feature having the chemical structure —NH—C—C(O)—, the building block molecule comprises a single amino acid derivative and the framework is the amino acid derivative.