Patent ID: 6440669
Filing Date: 2002-08-27
Classification: B01J,B05B,C07B,C07H,C40B

Abstract:
The method for forming an array of polynucleotides at discrete sites on a surface, said method comprising:(a) applying reagents to predetermined discrete sites on said surface, said reagents being selected from the group consisting of nucleotides and polynucleotides, said discrete sites being activated for attachment of nucleotides or polynucleotides thereto, (b) dispensing a volume of a liquid to uniformly coat the entire surface comprising said discrete sites with liquid, said volume of liquid being dispensed as particles of uniform diameter through a fluid ejection device activated by means of ultrasonic energy to provide a layer of liquid on said surface, said layer having a thickness of 20 to 500 microns, and (c) optionally repeating step (a) or step (b) wherein step (a) is repeated at least once when said reagents are nucleotides.