Patent ID: 7393936
Filing Date: 2008-07-01
Classification: C12N

Abstract:
1. A method for the recombinant production and purification of a correctly folded protein kinase selected from the group consisting of tyrosine protein kinases and serine/threonine kinases comprising: a) expressing a nucleic acid encoding said protein kinase in a microbial host cell, wherein inclusion bodies containing said protein kinase are formed in said host cell, b) isolating said protein kinase from the cell, solubilizing said protein kinase, and then renaturing said protein kinase to provide a renatured protein kinase, and c) purifying said renatured protein kinase by hydrophobic interaction chromatography with a hydrophobic adsorbent wherein said renatured protein kinase is applied directly to said hydrophobic adsorbent in an aqueous solution comprising at least a 1 M salt solution wherein the cation is selected from the group consisting of sodium, potassium and ammonium, and an anion selected from the group consisting of chloride, phosphate and sulfate, except that where the cation is ammonium, the anion is not sulfate, whereby at least 70% of said protein kinase recovered is correctly folded and is not bound to said hydrophobic adsorbent.