Patent ID: 8183344
Filing Date: 2012-05-22
Classification: A61K,A61P,C07K

Abstract:
1. A FVIII protein comprising the A1-, A2-, A3-, C1- and C2-domains of human Factor VIII, said FVIII protein having an amino acid sequence spacer between the A2- and A3-domains and a mutation at Arg740, wherein the spacer is of a sufficient length that upon thrombin activation, the FVIII protein becomes a heterodimer comprising an A1-domain and an A2-spacer-A3-C1-C2 chain, and wherein the mutation consists of a substitution of Arg at position 740 with A1a, and wherein the A2-domain remains covalently associated with the A3-, C1-, and C2-domains through the spacer.