Patent ID: 8048642
Filing Date: 2011-11-01
Classification: C07D,G01N

Abstract:
1. A method of detecting the presence of an analyte in a sample comprising the steps of: (a) providing a first binder capable of specifically binding the analyte, wherein the first binder is attached to a solid surface; (b) applying the sample to the solid surface under conditions that permit the first binder to bind to analyte present in the sample; (c) adding a second binder, wherein the second binder comprises a conjugate comprising a binder portion that binds to the analyte captured by the first binder and a choline esterase; (d) adding an apo-peroxidase, a heme choline ester, a peroxide, and a peroxide-activated signal generator; and (e) detecting the signal produced by the peroxide-activated signal generator.