Patent ID: 6730474
Filing Date: 2004-05-04
Classification: C12Q

Abstract:
A method of detecting a acid molecule having a chromosomal aberration, said method comprising:providing a pair of nucleic acid probes to detect chromosomal aberrations in hematological malignancies and to analyze a sample believed to contain said nucleic acid, said pair of nucleic acid probes having comparable size, said size being selected from the group consisting of 1 to 100 kb, 1 to 10 kb, 7 to 15 kb, 10 to 20 kb, 10 to 30 kb, 20 to 40 kb, 30 to 50 kb, 40 to 60 kb, 50 to 70 kb, 60 to 80 kb, 70 to 90 kb and 80 to 100 kb, and said pair of nucleic acid probes flanking a potential breakpoint in a single chromosome, each of said pair of nucleic acid probes being labeled with at least one different reporter molecule; hybridizing said pair of nucleic acid probes to said nucleic acid; and detecting the presence of a split signal that arises after a break within said potential breakpoint in the case of chromosomal aberration.