Patent ID: 9051666
Filing Date: 2015-06-09
Classification: B01J,B82Y,C12N,C12Q,C40B

Abstract:
1. A process for creating a mixture of double-stranded fragments in solution comprising: (a) synthesizing in situ or spotting a plurality of oligonucleotide sequences on a microarray device or bead device each having a solid or porous surface, wherein the plurality of oligonucleotide sequences are attached to the solid or porous surface and wherein each oligonucleotide sequence comprises a fragment of a target polynucleotide sequence; (b) amplifying each oligonucleotide sequence to form a plurality of double-stranded oligonucleotide sequences using a primer complementary to a primer region of at least one flanking sequence located at the 3′ end, the 5′ end, or the 3′ end and the 5′ end of each fragment, wherein the at least one flanking sequence is from about 7 to about 50 bases and comprises the primer region and a sequence segment having a restriction enzyme cleavable site; and (c) cleaving the primer regions from the plurality of double-stranded oligonucleotide sequences at the restriction enzyme cleavable sites, thereby to produce a plurality of double-stranded fragments of the target polynucleotide sequence, wherein the plurality of double-stranded fragments together comprise the target polynucleotide sequence.