Patent ID: 7132256
Filing Date: 2006-11-07
Classification: C12Q,G01N

Abstract:
1. A method for screening for a compound that specifically inhibits mPGES in mammalian cells responsive to increased levels of PGE2 upon induction with an external stimuli comprising the steps of: i) contacting a mammalian cell with said compound, ii) stimulating said mammalian cell with an external stimuli, iii) measuring the level of PGE2 and 6-keto PGF1α in said mammalian cell, and iv) comparing the levels of PGE2 and 6-keto PGF1α in said mammalian cell to the levels of PGE2 and 6-keto PGF1α in a mammalian cell where no compound was added and yet another mammalian cell where no compound and no external stimuli was added, wherein potency is determined as the ratio of PGE2 levels in the presence and the absence of compound in the cells exposed to the external stimuli and wherein specificity and cytotoxicity is determined as the ratio of 6-keto PGF1α levels in the presence and absence of compound in the cells exposed to the external stimuli.