Patent ID: 6146824
Filing Date: 2000-11-14
Classification: A61K,C07K,C12N,C12Y,G01N

Abstract:
A method for determining the ability of tumor cells to adhere to extracellular matrix, comprising:(a) incubating cells of the tumor, under conditions allowing cell adherence with a compound selected from the group consisting of:(i) a fragment of the thrombin B-chain that is active in inhibiting tumor cell adhesion to extracellular matrix comprising the sequence Arg-Gly-Asp in an exposed orientation; and(ii) a thrombin that was modified so that residues 187-189 (Arg-Gly-Asp) are in an exposed orientation and that is active in inhibiting tumor cell adhesion to extracellular matrix; and(b) determining the level of adherence of the cells to said compound, wherein if said cells adhere to said compound, it indicates the ability of the tumor cells to adhere to extracellular matrix.