Patent ID: 6117633
Filing Date: 2000-09-12
Classification: A61K,C07K

Abstract:
A method of identifying a nucleic acid sequence which is SEQ ID No 9 or its complement comprisinga) subtractively hybridizing breast cancer cell line cDNAs with cDNA from normal mammary epithelial cells to obtain subtracted fractions;b) combining said subtracted fractions and using them to probe a cDNA library;c) isolating cDNA clones obtained from step b) and randomly fragmenting them;d) transferring said DNA fragments to an expression vector to yield a genetic suppressor element library;e) introducing said genetic suppressor element library into normal cells;f) using the infected cells from step e) in a system which provides a means to identify an antisense oriented clone;g) selecting said antisense oriented clone; andh) isolating the cDNA of SEQ ID No 9 from the clones selected in step g).