Patent ID: 6406921
Filing Date: 2002-06-18
Classification: B01J,B82Y,C07K,C40B,G01N

Abstract:
An array device comprising:a substrate defining a surface; an array of spaced-apart protein immobilization regions over said surface, said protein immobilization regions being effective to immobilize one or more selected proteins, said immobilization regions being resistant non-specific protein binding after immobilization of said one or more selected proteins, one or more hydrophilic border regions surrounding each protein immobilization region and separating such protein immobilization regions from one another, said border regions each comprising (i) an ordered hydrophobic monolayer formed of alkyl chains having proximal ends which are chemisorbed or physisorbed to said surface within said protein immobilization regions, and opposite hydrophobic distal ends, (ii) a hydrophilic monolayer attached to said hydrophobic monolayer, said hydrophilic monolayer comprising hydrophilic chains, each hydrophilic chain having a proximal end by which said hydrophilic chain is linked to an alkyl chain distal end, and an opposite hydrophilic distal end, together said hydrophobic monolayer and hydrophilic monolayers forming said hydrophilic border regions which are effective to resist non-specific protein binding, wherein application of one or more of said selected proteins to selected protein immobilization regions is effective to form an array of protein regions having one or more selected proteins displayed within said protein immobilization regions such that each protein immobilization region is separated from other protein immobilization regions by hydrophilic border regions resistant to non-specific protein binding to form a protein array resistant to non-specific binding of proteins.