Patent ID: 6365364
Filing Date: 2002-04-02
Classification: A61K,C12N,C12Y

Abstract:
A method for making an isolated angiogenesis inhibitor having a molecular weight of between about 40 kDa to 50 kDa and having an amino acid sequence having at least about 85% identity to that of the amino acid sequence shown in SEQ ID NO. 1 or SEQ ID NO. 3, the method comprising:a) contacting plasminogen with an amount of Kringle-4 protease from Naja Nigricollis Nigricollis sufficient to cleave the plasminogen into fragments comprising fragments having a molecular weight of between about 50 kDa to 70 kDa, b) contacting the fragments with an amount of a second protease sufficient to cleave the fragments at between about the first 50 and 80 amino acids of the plasminogen; and c) isolating the angiogenesis inhibitor from the plasminogen fragments, wherein the second protease is elastase or plasmin.