Patent ID: 6699682
Filing Date: 2004-03-02
Classification: A61P,C07C,C07F,C12N,C12P

Abstract:
A method for ascertaining the inhibitory activity of a candidate inhibitor of fatty-acid amide hydrolase (FAAH), the method comprising the following steps:Step A: forming mixture â€œAâ€  by combining a FAAH having an amino acid residue sequence selected from the group consisting of SEQ ID NO:36, SEQ ID NO:40, and SEQ ID NO:43 and a fatty-acid primary amide substrate under reaction conditions; Step B: forming mixture â€œBâ€  by combining the mixture â€œAâ€  of said Step A with the candidate inhibitor under the reaction conditions of Step A; Step C: measuring the conversion of said fatty-acid primary amide substrate to a hydrolysis product within mixture â€œAâ€ ; Step D: measuring the conversion of said fatty-acid primary amide substrate to said hydrolysis product within mixture â€œBâ€ ; and then Step E: comparing the conversion of substrate to hydrolysis product in Step C to the conversion of substrate to hydrolysis product in Step D, wherein a finding of decreased conversion in Step D as compared to Step C indicates the inhibitory activity of the candidate inhibitor.