Patent ID: 6127154
Filing Date: 2000-10-03
Classification: B01J,B82Y

Abstract:
A method for producing a compound suitable for in vitro or in vivo usage as a diagnostic, therapeutic, purification, separation, or prophylactic agent that possesses a complementary structure to a specific site on a target molecular entity or portion thereof by site-specific molecular imprinting comprising the following steps:(i) selecting a particular molecular entity to which a compound having a complementary structure is to be produced;(ii) contacting such molecular entity with one or more complementary monomers under conditions wherein such monomers associate around one or more residues of such molecular entity;(iii) effecting polymerization of such associated monomers optionally in the presence of at least one crosslinking agent to produce a polymeric coating on the surface of said molecular entity and which possesses a complementary structure to said biological entity or a portion thereof;(iv) removing said molecule under conditions that result in a polymeric compound that possesses a complementary structure to said molecular entity or portion thereof; and(v) optionally effecting one or more cleavage and/or dissociation steps to produce compounds that are suitable for in vivo or in vitro usage as a diagnostic, therapeutic, purification, separation and/or prophylactic agent.