Patent ID: 9095549
Filing Date: 2015-08-04
Classification: A61K,G01N

Abstract:
1. A method of diagnosis of colorectal cancer in a diagnostic sample of a valid body tissue taken from a human subject, comprising: subjecting the diagnostic sample to two dimensional gel electrophoresis to yield a stained gel; detecting an increased or decreased concentration of each of a plurality of differentially expressed proteins in the diagnostic sample, compared with a control, normal human sample, by detecting an increased or decreased intensity of a protein-containing spot on the stained gel, compared with a corresponding control gel, wherein detecting further comprises identifying peptide profiles for each of the plurality of proteins using a mass spectrometer, the plurality of differentially expressed proteins comprising two or more proteins selected from the group consisting of: transforming growth factor-beta induced protein IG-H3; suppressor of G2 allele of SKP1 homolog (isoform 2); and hypothetical protein (part of URG4); the plurality of proteins optionally further comprising one or more proteins selected from the group consisting of: calponin-2; heat shock protein HSP90-beta; phosphoglycerate mutase 1; serpin C1 protein; haptoglobin precursor; serotransferrin; 26S proteasome subunit p40.5; aldo-keto reductase family 1 member B10; fructosamine-3-kinase; peripherin; alpha-2-macroglobulin; and apolipoprotein A IV.