Patent ID: 8367319
Filing Date: 2013-02-05
Classification: C12N

Abstract:
1. A method for identifying functional mutant ribosomes comprising: (a) transforming a set of host cells with a set of plasmids, each plasmid comprising a mutant rRNA gene and a selectable marker gene; wherein said mutant rRNA gene comprises at least one mutation in addition to a mutant Anti-Shine-Dalgarno sequence; and said selectable marker gene comprises a mutant Shine-Dalgarno sequence; and wherein said mutant Anti-Shine-Dalgarno and said mutant Shine-Dalgarno sequence are a mutually compatible pair; thereby forming a set of transformed host cells; (b) isolating from the set of transformed host cells those host cells which express the selectable marker gene product; and (c) sequencing the mutant rRNA gene from each host cell isolated in step (b), thereby identifying functional mutant ribosomes, wherein the functional mutant ribosomes comprise functionally important target regions of interest, which comprise nucleotide sequences of one or more nucleic acids or nucleotide motifs which are conserved in each mutant rRNA gene sequenced.