Patent ID: 6709596
Filing Date: 2004-03-23
Classification: B01D,B01J,G01N

Abstract:
A method for reversibly trapping and isolating carbohydrate moieties comprising:treating a carbohydrate-containing sample with hydrazine, resulting in hydrazine-activated carbohydrate-containing sample; preparing an aldehyde-containing trapping material; packing a trapping means with said aldehyde-containing trapping material; passing said hydrazine-activated carbohydrate-containing sample through said trapping means packed with said aldehyde-containing trapping material, thus binding and thereby trapping all hydrazine-activated carbohydrate moieties of said hydrazine-activated carbohydrate-containing sample therein; releasing said hydrazine-activated carbohydrate moieties from said aldehyde-containing trapping material by treating said aldehyde-containing trapping material and said hydrazine-activated carbohydrate moieties trapped therein, with additional amounts of anhydrous hydrazine; and retrieving said hydrazine-activated carbohydrate moieties from said trapping means after their release from said aldehyde-containing trapping material.