Patent ID: 6783957
Filing Date: 2004-08-31
Classification: C12P

Abstract:
A method for obtaining polypepoides in a cell-free system by which the reaction mixture is prepared with the use of a cell lysate or cell extract, the parameters of the cell-free system and the mode of synthesis are chosen, the type and parameters of at least one porous barrier are determined, the reaction mixture and the feeding solution are placed in the reaction module, and the synthesis is performed, wherein upon the parameters of the process are chosen, the types of the selected components determining the productivity of the synthesis are selected, the upper and lower limits of the range within which the concentrations of the selected components are changed during the synthesis are defined, the additional mixture containing the selected components is formed, the additional mixture is supplied to the reaction mixture or to the feeding solution, the synthesis is performed with changing concentrations of the selected components within the defined ranges while the concentrations of the other components are maintained constant.