Patent ID: 6579680
Filing Date: 2003-06-17
Classification: C07B,C07H,C12Q,Y10S

Abstract:
A method for detecting or identifying genetic polymorphisms, said method comprising:providing (1) a set of first probes, each (i) having a nucleic acid sequence that is attached to a solid support, and (ii) differing from each other in at least one base location, and (2) a set of second probes, each having a nucleic acid sequence that incorporates at least one artificial mismatch site relative to a first probe, such that said first probe and second probe are bound to each other in a duplex; providing each first probe with an emitter element, and each second probe with a quencher element; hybridizing an unlabeled sample nucleic acid target to said duplex of first and second probes; displacing said second probe from said duplex with said sample nucleic acid target, wherein said emitter element increases in emission intensity with such displacement.