Patent ID: 8940526
Filing Date: 2015-01-27
Classification: B01L,C12Q

Abstract:
1. A container for performing two-stage nucleic acid amplification on a sample in a closed system comprising one or more sealable ports, the sealable ports providing the only access from an exterior of the container such that when all of the one or more sealable ports is sealed, the container is fully closed, the one or more sealable ports including a sample injection port for introducing the sample into the container, a cell lysis zone configured for lysing cells or spores located in the sample, the cell lysis zone fluidly connected to the sample injection port, a nucleic acid preparation zone fluidly connected to the cell lysis zone, the nucleic acid preparation zone configured for purifying nucleic acids, a first-stage reaction zone fluidly connected to the nucleic acid preparation zone, the first-stage reaction zone comprising a first-stage reaction blister configured for first-stage amplification of the sample, and a second-stage reaction zone fluidly connected to the first-stage reaction zone, the second-stage reaction zone comprising a plurality of second-stage reaction chambers, each second-stage reaction chamber comprising a pair of primers configured for further amplification of the sample, the second-stage reaction zone configured for contemporaneous thermal cycling of all of the plurality of second-stage reaction chambers.