Patent ID: 6579682
Filing Date: 2003-06-17
Classification: G01N,Y10S,Y10T

Abstract:
A method for determining whether a compound affects LDL binding to proteoglycan and does not substantially affect LDL binding to LDL receptor, the method comprising:a) comparing the level of LDL binding to proteoglycan in the presence of the compound with the level of LDL binding to proteoglycan in the absence of the compound; b) comparing the level of LDL binding to LDL receptor in the presence of the compound with the level of LDL binding to LDL receptor in the absence of the compound; wherein the levels of binding in step (a) were determined by incubating mixtures comprising proteoglycan and LDL in the presence or absence of the compound under conditions where LDL binds to proteoglycan in the absence of the compound; and wherein the levels of binding in step (b) were determined by incubating mixtures comprising LDL and LDL receptor in the presence or absence of the compound under conditions where LDL binds to LDL receptor in the absence of the compound; and c) correlating any difference in the levels of binding in step (a) with the compound's ability to affect LDL-proteoglycan binding activity, and correlating any difference in the levels of binding in step (o) with the compound's ability to affect LDL-LDL receptor binding activity.