Patent ID: 8497128
Filing Date: 2013-07-30
Classification: C12N,C12P,C12R

Abstract:
1. A method for modifying moderately thermophilic Bacillus species that are facultative anaerobic and homolactic by genetic engineering comprising: introducing a DNA cloned in a thermosensitive plasmid system containing a pSH71 replicon or a homologue of the replicon, wherein the homologue comprises a DNA sequence encoding a polypeptide having a thermosensitive replication functionality (the RepA protein) having an amino acid sequence that is at least 90% identical to SEQ ID NO: 1, into cells of a moderately thermophilic culturing the cells on a selective medium at a permissive temperature for plasmid replication to select transformed cells capable of growing on said selective medium at said permissive temperature; and culturing said transformed cells on a selective medium at a non-permissive temperature for plasmid replication to select transformed cells capable of growing on said selective medium at said non-permissive temperature.