Patent ID: 8932834
Filing Date: 2015-01-13
Classification: C12N,C12P,C12Y

Abstract:
1. A method for producing an L-amino acid, which comprises culturing an Escherichia coli bacterium having an L-amino acid-producing ability in a medium to produce and accumulate an L-amino acid in the medium, and collecting the L-amino acid from the medium, wherein the bacterium has been modified so that an activity or activities of one or more enzyme(s) of the arginine succinyltransferase pathway is/are decreased, wherein the enzyme of the arginine succinyltransferase pathway is selected from the group consisting of arginine succinyltransferase, succinylarginine dihydrolase, succinylornithine aminotransferase, succinylglutamate-semialdehyde dehydrogenase, succinylglutamate desuccinylase, and combinations thereof, wherein the arginine succinyltransferase is selected from the group consisting of: (A) a protein having the amino acid sequence of SEQ ID No:2, (B) a protein having the amino acid sequence of SEQ ID No:2, but includes substitutions, deletions, insertions, or additions of 1-10 amino acid residues, and (C) combinations thereof; wherein the succinylarginine dihydrolase is selected from the group consisting of: (A) a protein having the amino acid sequence of SEQ ID No:4, (B) a protein having the amino acid sequence of SEQ ID No:4, but includes substitutions, deletions, insertions, or additions of 1-10 amino acid residues, and (C) combinations thereof; wherein the succinylornithine aminotransferase is selected from the group consisting of: (A) a protein having the amino acid sequence of SEQ ID No:6, (B) a protein having the amino acid sequence of SEQ ID No:6, but includes substitutions, deletions, insertions, or additions of 1-10 amino acid residues, and (C) combinations thereof; wherein the succinylglutamate-semialdehyde dehydrogenase is selected from the group consisting of: (A) a protein having the amino acid sequence of SEQ ID No:8, (B) a protein having the amino acid sequence of SEQ ID No:8, but includes substitutions, deletions, insertions, or additions of 1-10 amino acid residues, and (C) combinations thereof; wherein the succinylglutamate desuccinylase is selected from the group consisting of: (A) a protein having the amino acid sequence of SEQ ID No:10, (B) a protein having the amino acid sequence of SEQ ID No:10, but includes substitutions, deletions, insertions, or additions of 1-10 amino acid residues, and (C) combinations thereof; and wherein the L-amino acid is selected from the group consisting of L-lysine, L-threonine, and combinations thereof; and wherein the activity or activities of the one or more enzyme(s) of the arginine succinyltransferase pathway is/are decreased by a method selected from the group consisting of decreasing expression of a gene or genes encoding for the one or more enzymes, disrupting a gene or genes encoding for the one or more enzymes, and combinations thereof.