Patent ID: 8114982
Filing Date: 2012-02-14
Classification: C12N

Abstract:
1. A DNA comprising a polynucleotide that encodes at east a first modified miR-30 precursor and a second modified miR-30 precursor, wherein the first and second modified miR-30 precursors are each at least 80 nucleotides long and comprise a stem-loop structure, wherein the first modified miR-30 precursor further comprises a first miRNA sequence of 20-22 nucleotides on the stem of the stem-loop structure complementary to a portion of a first target sequence wherein the first target sequence is a first naturally occurring mRNA sequence, wherein the second modified miR-30 precursor further comprises a second miRNA sequence of 20-22 nucleotides on the stem-loop structure complementary to a second target sequence, and wherein the first and second modified miR-30 precursors each independently further comprise a sequence complementary to the first and second miRNA sequence of 20-22 nucleotides, respectively, that base-pairs to the first and second miRNA sequence of 20-22 nucleotides, respectively, in the stem of the stem-loop structure, and wherein the first modified miR-30 precursor and the second modified miR-30 precursor are 13-25 nucleotides apart.