Patent ID: 6511806
Filing Date: 2003-01-28
Classification: C12Q,G01N

Abstract:
A method for separating living neoplastic cells that are resistant to a cytotoxic compound from dead cells, living stromal cells and living neoplastic cells that are sensitive to the cytotoxic compound in a mixed population of cells from a tumor sample, the method comprising the steps of:a) contacting the mixed population of cells with the cytotoxic compound for a time and at a concentration wherein the stromal cells and neoplastic cells that are sensitive to the cytotoxic compound undergo apoptosis; b) contacting the mixed population of step (a) with a vital stain or fluorescent dye; c) contacting the mixed population of cells of step (b) with Annexin V; d) contacting the mixed cell population of step (c) with a detectably-labeled immunological reagent that specifically binds to Annexin V; and e) selecting the cells in the mixed population of step (c) that are not stained with the vital stain and that do not bind the immunological reagent.