Patent ID: 9040301
Filing Date: 2015-05-26
Classification: A01H,C12N

Abstract:
1. A method of separating fluid-suspended embryos from immature embryogenic tissue comprising the steps of: a) providing a suitable separator container, said container containing fluid having a density lower than of the embryos to be separated, being essentially cylindrical in shape, having an essentially flat bottom wall and an essentially vertical axis, and comprising a conduit in communication with the fluid in the container at an axial region of the bottom wall during operation, and providing a rotation object positioned inside the container for inducing an axisymmetric rotating flow; b) creating a sink vortex at the axial region of the bottom wall by draining fluid from said conduit, and inducing an axisymmetric rotating flow in the fluid relative to the bottom wall by rotating the rotation object, thus: c) introducing the fluid-suspended embryos and immature embryogenic tissue to be separated into the fluid present in the separator container at a location away from the bottom wall, thus: d) collecting embryos from said axial region of the bottom wall in the fluid drained from the conduit, whereby the embryos collected are essentially separated from immature embryogenic tissue.