Patent ID: 6368818
Filing Date: 2002-04-09
Classification: A61K

Abstract:
A method for visualizing the structure of cellular organelles and/or cytoskeletons selected from the group consisting of centrosomes and microtubules, in tissue or in cell suspension, said method comprising the steps of:(a) obtaining a tissue sample or cell sample, said sample containing a plurality of cells, wherein said tissue sample or cell is selected from the group consisting of normal mammalian tissue or cell, pathologic mammalian tissue or cell, and neoplastic tissue or cell; (b) contacting said tissue sample or cell sample with one or more crystallizing agents under conditions that permit formation of visible crystals through the reduction of said crystallizing agent by the enzymes in said cellular organelles and/or cytoskeletons selected from the group consisting of centrosomes and microtubules of said cells or tissues; and (c) visualizing said crystals formed in said cellular organelles and/or cytoskeletons selected from the group consisting of centrosomes and microtubules.