Patent ID: 6758865
Filing Date: 2004-07-06
Classification: A61F,A61L,Y10S

Abstract:
A method of preparing a soft tissue xenograft for implantation into a human, which comprises:a. removing at least a portion of a soft tissue from a non-human animal to provide a xenograft, b. washing the xenograft in water and alcohol; c. subjecting the xenograft to a cellular disruption treatment; and d. treating the xenograft with a glycosidase to remove a plurality of first surface carbohydrate moieties, whereby the xenograft has substantially the same mechanical properties as a corresponding portion of a native soft tissue, where the glycosidase has a concentration in a range of about 1 mU/ml to about 1000 U/ml; wherein (i) the cellular disruption treatment comprises freeze/thaw cycling or exposure to gamma radiation; and (ii) further comprises the steps of:a) prior to step c, piercing the xenograft; b) following step c, treating the xenograft with a second enzyme; c) following step c, treating the xenograft with one or more agents selected from the group consisting of anticalcification agents, antithrombotic agents, antibiotics, and growth factors; d) following step c, sterilizing the xenograft; e) following step c, treating the xenograft with polyethylene glycol; f) following step d, treating a plurality of second surface carbohydrate moieties on the xenograft with a plurality of capping molecules to cap at least a portion of the second surface carbohydrate moieties, whereby the xenograft is substantially non-immunogenic; and g) following step c, exposing the xenograft to a crosslinking agent in a vapor form.