Patent ID: 6143525
Filing Date: 2000-11-07
Classification: C12N

Abstract:
A complex inducible promoter system from a phage of a lactic acid bacterium, said complex inducible promoter system comprising a repressor protein alpha-helix-turn-alpha-helix region encoding sequence and an operator sequence with internal dyad symmetry, said complex inducible promoter system comprising the following elements in the spacing order and direction of transcription as in SEQ ID NO: 3:a gene encoding a repressor protein with the amino acid sequence encoded by open reading frame (ORF) 27, the ribosome binding site (RBS) of ORF 27,the -35 and -10 promoter sequences of P2,the operator sequence O1,the operator sequence O2,the -35 and -10 promoter sequences of P1,a gene encoding a tec protein with the amino acid sequence encoded by ORF 28,the Shine-Dalgarno (SD) sequence of said gene encoding a tec protein, wherein the elements are linked by intervening sequences and optionally flanked by flanking sequences, said intervening sequences and optionally said flanking sequences having the same length as in SEQ ID NO: 3 but not necessarily the same composition.