Patent ID: 8288095
Filing Date: 2012-10-16
Classification: C12N,C12Q,G16B

Abstract:
1. A method of polynucleotide sequencing, comprising: (a) providing a mixture of circular polynucleotides, wherein each of said circular polynucleotides comprises: (i) a first Type IIs restriction endonuclease recognition site; (ii) a target polynucleotide fragment; and (iii) a second Type IIs restriction endonuclease recognition site, (b) cleaving the different target polynucleotide fragments contained in said mixture of the circular polynucleotides with said first Type IIs restriction endonuclease and said second Type IIs restriction endonuclease, thereby producing restriction fragments comprising segments from the ends of the different target polynucleotide fragments; and (c) determining sequences of the segments from the ends of the different target polynucleotide fragments after the cleaving step.