Patent ID: 6610543
Filing Date: 2003-08-26
Classification: C12N

Abstract:
A method for culturing biological material in vitro, the biological material being selected from the group consisting of follicles in the course of development for maturation of oocytes contained in said follicles, cells of a male germinal line to be matured, oocytes to be fertilized by a spermatozoa, and embryos to be cultured, said method comprising culturing said biological material in a culture composition comprising at least two growth factors in combination with at least one glucocorticoid compound, and, optionally with at least one co-enzyme of energy metabolism, optionally after thawing of the follicles, cells or embryos,wherein said at least two growth factors are selected from the group consisting of: hepatic growth factors, transformation growth factors &agr;, granulocyte and macrophage colony stimulation factor, epidermal growth factor, growth and differentiation factor (GDF), and insulin-like growth factors.