Patent ID: 9120848
Filing Date: 2015-09-01
Classification: A61K,C07K

Abstract:
1. A method of covalent coupling of a non-reducing polysaccharide to a protein comprising the following steps: providing a non-reducing polysaccharide; activating the non-reducing polysaccharide to generate an activated polysaccharide having aldehyde groups at multiple interior sites of said non-reducing polysaccharide; introducing a protein to provide an aqueous mixture of the activated polysaccharide and the protein; removing the water from the aqueous mixture by lyophilization to produce a dry lyophilized sample; sealing said dry lyophilized sample under a vacuum or under an atmosphere of dry inert gas having low water vapour pressure; incubating said dry lyophilized sample or under an atmosphere of dry inert gas having low water vapour pressure under vacuum at elevated temperature to cause a chemical reaction forming a protein-polysaccharide conjugate.