Patent ID: 7632637
Filing Date: 2009-12-15
Classification: C12N,G01N

Abstract:
1. A phage binding and orientation method comprising the steps of: providing a long-chained alkyl amine immobilized on a silica-based substrate: binding a nucleic acid complexing agent to the long-chained alkyl amine, wherein the nucleic acid complexing agent is an unsymmetrical cyanine dye configured to penetrate a phage head and bind to nucleic acid therein; binding a silanated amine to the silica-based substrate, wherein a silane portion of the silanated amine binds to the silica-based substrate and a primary amine portion of the silanated amine is available upon activation for binding to phage having head and tail ends; activating the primary amine portion of the silanated amine by reaction with a dialdehyde; adding the phage, wherein the nucleic acid complexing agent binds to the nucleic acid within the phage head to orient the phage and the activated primary amine then binds to the phage head; and reacting any remaining activated primary amine portions of the silanated amine with an alkyl-amine compound.