Patent ID: 8680017
Filing Date: 2014-03-25
Classification: C12N

Abstract:
1. A method of identifying nucleic acid aptamers for a target molecule comprising: obtaining an at least partially purified target molecule; obtaining a nucleic acid ligand library, said nucleic acid ligand library comprising a plurality of nucleic acid molecules; mixing the target molecule with the nucleic acid ligand library such that the target molecule binds to one or more aptamers present in the nucleic acid ligand library; digesting unbound nucleic acid molecules with one or more nucleases; separating the nucleic acid molecules bound to the target molecule from at least a portion of the unbound nucleic acid molecules, wherein said separation is accomplished by: a) digesting unbound nucleic acid molecules; or b) digesting unbound nucleic acid molecules in combination at least one physical separation means; disassociating the target molecule from the one or more nucleic acid molecules; and amplifying separated nucleic acid molecules; wherein the nucleic acid ligand library comprises a plurality of nucleic acid molecules comprising the sequence 5′-Z wherein the nucleic acid ligand library comprises a plurality of nucleic acid molecules comprising the sequence 5′-TTTGGGGGGGGTTTTTTTTGGG-N