Patent ID: 6387647
Filing Date: 2002-05-14
Classification: C12Q

Abstract:
A method for screening for the presence or absence of inhibition of cytochrome P450 or cytochrome P450 related enzymes or electron-transfer proteins by a test sample which comprises:(a) adding said test sample to a culture of a Saccharomyces cerevisiae strain having a disrupted cytochrome P450 reductase gene; wherein said cytochrome P450 reductase gene is involved in the ergosterol synthetic pathway (b) adding said test sample to a second culture of a Saccharomyces cerevisiae strain having an erg6 mutation; (c) incubating said test samples in said cultures for such time under such conditions sufficient to observe yeast cell growth in corresponding cultures containing no test sample; (d) comparing the extent of growth inhibition in the culture containing the disrupted P450 reductase gene with the extent of growth inhibition in the culture containing the erg6 mutant; and (e) determining the presence of said cytochrome P450 or cytochrome P450 related enzymes or electron-transfer proteins inhibition by observation of whether growth inhibition in the disrupted reductase growth culture exceeds growth inhibition in the erg6 culture.