Patent ID: 8541559
Filing Date: 2013-09-24
Classification: A61K,A61P,C07H,C12N

Abstract:
1. A process for producing an nucleotide composition comprising aggregated oligonucleotides, said process comprising the steps of: (a) providing at least two oligonucleotides in solution I, wherein said oligonucleotides comprise at their 5′ end at least 3 and at most 15 guanosine entities and at their 3′ end at least 3 and at most 15 guanosine entities, wherein each oligonucleotide of said oligonucleotides comprise 20 to 40 nucleotides, and wherein said solution I comprises a pH of 10 to 13; (b) disaggregating said oligonucleotides, wherein said disaggregating comprises the steps of (c) adjusting the pH of said solution I to 5 to 8; and (d) aggregating said oligonucleotides, wherein said aggregating comprises the steps of: