Patent ID: 6251665
Filing Date: 2001-06-26
Classification: A61K,C12N

Abstract:
A method for producing cancer-specific programmed super antigen presenting cells (pSAPCs) capable of stimulating an immune response to said cancer, the method comprising the steps of:a) isolating CD34 positive hematopoietic stem cells;b) incubating the stem cells in a cell culture medium comprising serum, GM-CSF, IL-4, and SCF, whereby the stem cells are directed to mature into programmable antigen presenting cells (pAPCs);c) mixing the pAPCs with tumor cell derived material from said cancer selected from the group consisting of tumor cell lysate, tumor cell RNA, tumor cell poly A+ RNA, cDNA derived from tumor cell RNA, and cDNA derived from tumor cell RNA incorporated into an expression vector;d) incubating the pAPCs with the tumor cell derived material, whereby loaded dendritic cells are produced; ande) incubating the loaded dendritic cells with TNF-.alpha., whereby cancer-specific pSAPCs are produced.