Patent ID: 7642065
Filing Date: 2010-01-05
Classification: C12Q

Abstract:
1. A method to determine the efficacy of a compound which modulates the activity of cholesterol ester transfer protein (CETP) as present in a bodily fluid of a subject, which method comprises obtaining a first sample of bodily fluid from said subject before, and a second sample of bodily fluid from said subject after, administration of said compound to said subject; contacting the first and second samples with a donor wherein said donor does not comprise apoA-I, comprising a labeled CETP substrate, and an acceptor, to which said substrate may be transferred by CETP activity, to form first and second reaction mixture solutions, respectively, wherein at least about 89% v/v of said reaction mixture solutions are said samples of bodily fluid; and detecting transfer of said substrate from said donor to said acceptor to determine the activity of CETP in each sample; wherein said samples contain an endogenous CETP inhibitor or activator; wherein the efficacy of said compound is determined by comparing the CETP activity of the second sample to that of the first sample.