Patent ID: 9121066
Filing Date: 2015-09-01
Classification: C12N,C12Q

Abstract:
1. A method of implanting an embryo in a female undergoing in vitro fertilization, comprising the steps of: a) collecting at least one oocyte with its cumulus cells from said female; b) measuring, in said cumulus cells surrounding said oocyte, an expression level of each of the 5 microRNA hsa-let-7a-1, hsa-mir-182, hsa-mir-21, hsa-mir-320a and hsa-mir-210; c) comparing the expression level of each of the 5 microRNA in the cumulus cells with control expression levels of the 5 microRNA from cumulus cells associated with competent oocytes; d) assessing said oocyte as having a higher probability of being competent if the 5 microRNA are not differentially expressed when compared to cumulus cells associated with competent oocytes; e) fertilizing said oocyte having a higher probability of being competent in vitro to generate an embryo; and f) implanting said embryo in said female.