Patent ID: 6207158
Filing Date: 2001-03-27
Classification: A61P,C07K,H01G,Y10S

Abstract:
An isolated protein comprising a parasitic helminth MIF protein, wherein said protein elicits an immune response against a parasitic helminth MIF protein, and wherein said protein is encoded by a nucleic acid molecule that hybridizes to a nucleic acid sequence complementary to a nucleic acid sequence selected from the group consisting of SEQ ID NO:3, SEQ ID NO:6, SEQ ID NO:9, SEQ ID NO:17, and SEQ ID NO:19 immobilized on a filter under conditions comprising the steps of:(a) contacting said nucleic acid molecule with a solution of 5X SSPE, 1% N-lauroyl sarcosine, 0.5% dried milk in water, and 0.1 mg/ml denatured salmon sperm DNA at 42.degree. C.;(b) washing the filter 3 times in a wash solution containing 5X SSPE, 1% N-lauroyl sarcosine at 42.degree. C.; and(c) further washing the filter two times in a wash solution containing 2X SSPE, 1% N-lauroyl sarcosine at 42.degree. C.