Patent ID: 8741570
Filing Date: 2014-06-03
Classification: B01L,C12Q,G01N,Y10T

Abstract:
1. A method for the thermo-optical determination of the size of fluorescently labeled nucleic acids in a reaction solution, comprising the steps of: (i) providing a reaction solution comprising fluorescently labeled nucleic acids; (ii) irradiating a laser light beam into the solution to obtain a spatial temperature distribution in the solution around the irradiated laser light beam; (iii) exciting fluorescently the fluorescently labeled nucleic acids and detecting the fluorescence at least at one position or at around one position in the solution of the fluorescently excited nucleic acids, wherein the detection of fluorescence is performed at a predetermined time of 1 to 250 s after the start of the laser irradiation; and (iv) determining the size of the fluorescently labeled nucleic acids from the detected fluorescence intensity; wherein the reaction solution is a nucleic acid amplification solution or is a nucleic acid restriction solution.