Patent ID: 6455759
Filing Date: 2002-09-24
Classification: C07K,C12N

Abstract:
A transgenic angiosperm plant comprising a DNA construction inserted into the genome of the plant, the DNA construction comprising 5â€² to 3â€²:a promoter operable in plant cells; a coding region encoding a fusion protein, the fusion protein comprising in a common reading frame, in order: a coding region for a first protein of interest; a coding region for a plant ubiquitin monomer; and a coding region for second protein of interest; a transcriptional terminator operable in plants, the coding region encoding the fusion protein effective in the cells of the transgenic plant to result in translation of the fusion protein in the cells of the plant, the fusion protein being cleaved at the ubiquitin monomer by enzymes present in the cells of the plant separating the first and second proteins of interest from each other in the cells of the transgenic plant, neither the first nor the second proteins of interest being ubiquitin.