Patent ID: 7892794
Filing Date: 2011-02-22
Classification: C12Q,G01N

Abstract:
1. A method for detecting a biological particle from gaseous sample, the method comprising the steps of: a) providing a sample chamber and a first and a second electrode, the first and the second electrode and the sample chamber being so positioned that at least a part of the sample chamber is between the first and the second electrode, a distance between the first and the second electrode being at most 20 mm, b) providing a gaseous sample in the sample chamber, c) applying a first potential to the first electrode and a second potential to the second electrode, thus resulting in a potential difference and an electric field between the first and second electrode, to assist electrostatic collection, in the sample chamber, of a biological particle from the gaseous sample, d) contacting the collected biological particle with a first liquid reagent, thus obtaining the reaction mixture, e) exposing said reaction mixture to an alternating electric field in said sample chamber, said alternating electric field having a sufficient amplitude so as to enable extraction of biological material from the biological particle, f) performing nucleic acid amplification of a target nucleic acid sequence, and g) measuring the presence of the amplified target nucleic acid sequence and/or products resulting from amplification of the target nucleic acid sequence.