Patent ID: 6586021
Filing Date: 2003-07-01
Classification: A61K,Y10S

Abstract:
A process for extraction and purification of biologically useful molecules &bgr;-amyrin, ursolic acid, methyl palmitate, and lupeol from mangrove plant Salvadora persica comprising the steps of:(a) drying said plant; (b) cutting said dried plant into small pieces; (c) immersing said small plant pieces into about 85-95% aqueous methanol for about 5-15 days to obtain an extract; (d) evaporating said aqueous methanol from said obtained extract under reduced pressure at a temperature of from 45Â° to 55Â° C.; (e) cold-percolating said extract at room temperature; (f) repeating steps (c)-(e) at least twice to obtain a concentrated crude extract; (g) fractionating said concentrated crude extract into a petroleum ether fraction, a chloroform fraction, an n-butanol fraction, and an aqueous fraction using separate funnels; (h) identifying said aqueous fraction as the fraction containing said biologically useful molecules; (i) isolating said biologically useful molecules via chromatography; and (j) identifying said compounds via spectroscopy.