Patent ID: 8237014
Filing Date: 2012-08-07
Classification: C12N,C12P,C12Y,Y02A,Y02E

Abstract:
1. A method of saccharification comprising steps of: treating a plant part obtained from at least one transgenic plant with at least one thermostable lignocellulolytic enzyme, wherein the thermostable lignocellulolytic enzyme is expressed from a transgene present in the at least one transgenic plant, under conditions to activate the at least one thermostable lignocellulolytic enzyme thereby deconstructing lignocellulose to form a hydrolysate mixture; and incubating the hydrolysate mixture under conditions that promote conversion of fermentable sugars of the hydrolysate mixture, wherein the genome of the at least one transgenic plant is augmented with: a recombinant polynucleotide encoding the at least one thermostable lignocellulolytic enzyme operably linked to a promoter sequence, wherein the at least one thermostable lignocellulolytic enzyme exhibits optimal activity at a temperature greater than about 50° C., wherein the at least one thermostable lignocellulolytic enzyme is produced at a level greater than 0.5% total soluble protein, greater than 5% total protein, greater than 10% total soluble protein, or greater than 20% total soluble protein, and wherein, prior to the treating step, the plant part is not pretreated or is pretreated under conditions of less heat or less acid, or that are otherwise less harsh or produce fewer toxic by-products than conditions used in pretreatment of biomass from non-transgenic plants.