Patent ID: 8143381
Filing Date: 2012-03-27
Classification: C07K,Y02P

Abstract:
1. A method for extracting a membrane protein from a biological sample comprising contacting said sample with a water-miscible ionic liquid which is N-(3-hydroxypropyl)-N-methylmorpholinium bistrifluoromethysulfonylimide, 4-(2-methoxyethyl)-4-methylmorpholinium bromide, 4-methyl-4-propylmorpholinium bromide, 4-(2-ethoxyethyl)-4-methylmorpholinium bromide, 4-(3-hydroxypropyl)-4-methylmorpholinium bromide, 4-(2-hydroxyethyl)-4-methylmorpholinium bromide, 4-(3-methoxypropy)-4-methylmorpholinium bromide, 4-butyl-4-propylmorpholinium bromide, trihexyl(tetradecyl)phosphonium tetrafluoroborate, 1-decyl-3-methylimidazolium bromide, 1-dodecyl -3-methylimidazolium chloride, 3-methyl-1-octadecylimidazolium hexafluorophosphate, or a mixture thereof or a mixture comprising said water-miscible ionic liquid and at least one further solvent; and extracting said membrane protein, optionally with mechanical action.