Patent ID: 7923211
Filing Date: 2011-04-12
Classification: G01N,Y10S,Y10T

Abstract:
1. A method of determining whether or not the binding of a target molecule to a ligand is specific, said method comprising: (i) contacting a sample comprising the target molecule with a solid phase carrier comprising an immobilized ligand to obtain a once-treated liquid, (ii) extracting the target molecule bound to the ligand immobilized on the solid phase carrier in (i) to obtain a ligand-immobilized solid phase carrier first extract, (iii) contacting the once-treated liquid obtained in (i) with another solid phase carrier comprising the same immobilized ligand as in step (i) to obtain a twice-treated liquid, (iv) extracting the target molecule bound to the ligand immobilized on the solid phase carrier in (iii) to obtain a ligand-immobilized solid phase carrier second extract, (v) comparing and/or analyzing the target molecule contained in the first extract and the target molecule contained in the second extract, and (vi) identifying the target molecule as being specific for the ligand if the target molecule is detected in the first extract and is not detected in the second extract or the target molecule is detected in the second extract at a significantly lower level than in the first extract.