Patent ID: 8217160
Filing Date: 2012-07-10
Classification: C12Q

Abstract:
1. A kit for assaying an analyte, the kit comprising: a) a support; b) capture oligonucleotides coupled to the support, the capture oligonucleotides comprising a molecular recognition sequence having at least one non-standard base; c) a first primer comprising a tagging sequence and a sequence complementary to a first sequence of the analyte, wherein the tagging sequence is complementary to the molecular recognition sequence and includes a non-standard base that is complementary to the non-standard base of the molecular recognition sequence; d) a second primer that includes a sequence complementary to a second sequence of the analyte, wherein the second primer includes a non-standard base; and e) a non-standard nucleotide triphosphate having a non-standard base that is complementary to the non-standard base of the second primer, wherein the non-standard nucleotide triphosphate further comprises a label or a coupling agent.