Patent ID: 7816140
Filing Date: 2010-10-19
Classification: A01K,A61K,A61P,C07K,C12N

Abstract:
1. An ex vivo method of inducing division and differentiation of a hematopoietic stem cell, comprising: expressing in a hematopoietic stem cell, at least one heterologous nucleic acid comprising a polynucleotide sequence that encodes an osteogenic growth factor comprising a secretable analog of human fibroblast growth factor-2 (FGF-2) which promotes self-renewal by the stem cell and enhances bone growth in vivo and angiogenesis in vivo and is encoded by a nucleic acid sequence comprising in a 5′ to 3′ direction: a polynucleotide sequence that encodes a bone morphogenetic protein 2/4 (BMP2/4) hybrid secretion signal sequence; and a polynucleotide sequence that encodes a mature human FGF-2 polypeptide, wherein the mature human FGF-2 polypeptide comprises a mutation that results in the substitution of an alanine for a cysteine in amino acid position 70 of FGF-2, and a mutation that results in the substitution of an asparagine for a cysteine in amino acid position 88 of FGF-2 and; introducing the hematopoietic stem cell or a progeny thereof into a subject, thereby enhancing bone growth and bone repair in the subject.