Patent ID: 6509182
Filing Date: 2003-01-21
Classification: A21D,C11D,C12N

Abstract:
An isolated lipolytic enzyme, comprising:a) a polypeptide encoded by the lipolytic enzyme encoding part of the DNA sequence cloned into a plasmid present in Escherchia coli deposit number DSM 13538, b) a polypeptide having an amino acid sequence of the mature peptide shown in SEQ ID NO: 2; c) an analogue of the polypeptide defined in (a) or (b) which has at least 95% homology with said polypeptide, or d) a polypeptide which is encoded by a nucleic acid sequence which hybridizes with a complementary strand of the nucleic acid sequence of SEQ ID NO: 1 encoding the mature polypeptide under hybridization conditions comprising prehybridizing in a solution of 5Ã— SSC, 5 Ã— Denhardt's solution, 0.5% SDS and 100 &mgr;g/ml of denatured sonicated salmon sperm DNA, followed by hybridization in the same solution for 12 hours at approx. 45Â° C., followed by washing in 2Ã— SSC, 0.5% SDS for 30 minutes at a temperature of at least 70Â° C.