Patent ID: 8588503
Filing Date: 2013-11-19
Classification: G01N,G06T

Abstract:
1. A method of analyzing a plurality of images of a biological sample comprising a population of at least one cell from time-lapse imaging, comprising the steps of: detecting out of focus images by: decomposing each image, using a wavelet transform, Fourier transform, or other frequency decomposing transform, into a plurality of sub-images each representing a specific frequency channel and one sub-image representing an approximation channel, calculating a defocus ratio for each image as the ratio between the energy of the approximation channel sub-image and the sum of the energy of the sub-images representing middle frequency channels, and comparing the defocus ratio of each image with a predetermined defocus threshold value wherein images with a defocus ratio exceeding the defocus threshold value are identified as out of focus, and segmenting and tracking the at least one cell in images not identified as out of focus; wherein each image is decomposed using a wavelet transform and the energy of the sub-images is defined as the mean magnitude of wavelet coefficients for each sub-image.