Patent ID: 6511815
Filing Date: 2003-01-28
Classification: G01N

Abstract:
A method for measuring the ability of a compound to affect the binding of molecules to a steroid hormone receptor, comprising:a. providing a solution comprising a fluorescence-emitting compound that binds to the steroid hormone receptor at a first domain; a fluorescence-labeled nucleic acid that binds to the steroid hormone receptor at a second domain; and the steroid hormone receptor; b. measuring the fluorescence polarization of said fluorescence-emitting compound and said fluorescence-labeled nucleic acid present in the solution from step a) at excitation and emission wavelengths corresponding to the excitation and emission wavelengths of each of said fluorescence-emitting compound and said fluorescence-labeled nucleic acid; c. incubating the solution of step a) with at least one unlabeled compound that may affect the binding of said fluorescence-emitting compound or said fluorescence-labeled nucleic acid to said steroid hormone receptor; d. measuring the fluorescence polarization of said fluorescence-emitting compound and said fluorescence-labeled nucleic acid present in the solution from step c) at excitation and emission wavelengths corresponding to the excitation and emission wavelengths of each of said fluorescence-emitting compound and said fluorescence-labeled nucleic acid; and, e. comparing the fluorescence polarization measurements of step b) with step d) to determine if said unlabeled compound affects the binding of said fluorescence-emitting compound or said fluorescence-labeled nucleic acid to the steroid hormone receptor.