Patent ID: 8318497
Filing Date: 2012-11-27
Classification: C12N

Abstract:
1. A recombinant genetic construct comprising: a) a vector comprising a first promoter/activator sequence disposed upstream of a first nucleotide sequence encoding a first poison protein, and a second promoter/activator sequence disposed upstream of a second nucleotide sequence encoding an antidote to a second poison protein different from said first poison protein, or b) a vector comprising a promoter/activator sequence disposed upstream of a first nucleotide sequence encoding a first poison protein and disposed upstream of a second nucleotide sequence encoding an antidote to a second poison protein different from said first poison protein, wherein the poison/antidote proteins are selected from the group consisting of CcdB/CcdA, Kid/Kis, Doc/Phd, RelE/RelB, PasA/PasB/PasC, MazE/MazF and ParE/ParD.