Patent ID: 6673547
Filing Date: 2004-01-06
Classification: C12Q,H01J

Abstract:
A DNA analysis method for analyzing DNA polymorphism, comprising the steps of:generating plural kinds of multiply-charged ions of a test DNA fragment by ionization, where each of the multiply-charged ions has five or more charges; performing a mass spectrometry an the multiply-charged ions formed by the ionization so as to measure a mass spectrum of the test DNA fragment; predicting possible mass spectrum patterns in each of two cases, where one of the two cases is that the test DNA fragment is polymorphic and an other of the two cases is that the test DNA fragment is not polymorphic, based on both an information about the number of each of four different nucleic acid bases that constitute the test DNA fragment and an information about a polymorphism point; and comparing a plurality of the predicted mass spectrum patterns with the measured mass spectrum to determine a nucleic acid base on the polymorphism point.