Patent ID: 6548261
Filing Date: 2003-04-15
Classification: C12Q,G01N

Abstract:
A screening method for testing compounds for their ability to inhibit a deleterious event associated with re-entry into the cell cycle comprising:a) providing, in any order: i) a reaction vessel; ii) a plurality of mammalian neuronal cells transfected with two oncogenes such that said transfected cells exhibit said deleterious event associated with reentry into the cell cycle, wherein said oncogenes are Myc and Ras; and iii) a test compound; b) combining said transfected cells and said test compound within said reaction vessel under conditions such that said compound is free to interact with said transfected cells; c) detecting said interaction under conditions such that the amount of inhibition of said deleterious event associated with re-entry into the cell cycle is measured; and d) determining the viability of said cells after contact with said test compound.