Patent ID: 7993912
Filing Date: 2011-08-09
Classification: G01N,Y10S

Abstract:
1. A biosensor for detecting a test molecule specifically binding to a physiologically active substance, which comprises: (1) (a) a first reaction region on which the physiologically active substance has been immobilized for performing a binding reaction between the physiologically active substance and the test molecule and a physiologically active reaction caused by the physiologically active substance, and (b) a second reaction region on which a molecule that specifically binds to a reaction product resulting from the physiologically active reaction has been immobilized for performing a binding reaction between the reaction product and the molecule that specifically binds to the reaction product, in the same area; and (2) an assay region for detecting changes in the binding reaction in the first reaction region and in the binding reaction in the second reaction region, the assay region being provided in such a way that the assay region can monitor the binding reactions in the first and second reaction regions by using surface plasmon resonance (SPR) assay, wherein the reaction product moves spontaneously from the first reaction region to the second reaction region by diffusion.