Patent ID: 8741814
Filing Date: 2014-06-03
Classification: A61K,A61P,C07K,C12N,C40B

Abstract:
1. A phage particle displaying on its surface a human dimeric T cell receptor (TCR) polypeptide pair constituted by: a TCR α chain variable domain sequence fused to the N terminus of a TCR α chain constant domain extracellular sequence; and a TCR β chain variable domain sequence fused to the N terminus of a TCR β chain constant domain extracellular sequence, wherein the TCR α chain constant domain extracellular sequence and the TCR β chain constant domain extracellular sequence are linked by a disulfide bond between cysteine residues substituted for: Thr 48 of exon 1 of TRAC*01 at position 10 with reference to the position numbering of SEQ ID NO: 1 and Ser 57 of exon 1 of TRBC1 *01 or TRBC2*01 at position 10 with reference to the position numbering of SEQ ID NO: 5; Thr 45 of exon 1 of TRAC*01 at position 10 with reference to the position numbering of SEQ ID NO: 2 and Ser 77 of exon 1 of TRBC1 *01 or TRBC2*01 at position 10 with reference to the position numbering of SEQ ID NO: 6; Tyr 10 of exon 1 of TRAC*01 at position 10 with reference to the position numbering of SEQ ID NO: 3 and Ser 17 of exon 1 of TRBC1 *01 or TRBC2*01 at position 10 with reference to the position numbering of SEQ ID NO: 7; Thr 45 of exon 1 of TRAC*01 at position 10 with reference to the position numbering of SEQ ID NO: 2 and Asp 59 of exon 1 of TRBC1 *01 or TRBC2*01 at position 10 with reference to the position numbering of SEQ ID NO: 8; or Ser 15 of exon 1 of TRAC*01 at position 10 with reference to the position numbering of SEQ ID NO: 4 and Glu 15 of exon 1 of TRBC1 *01 or TRBC2*01 at position 10 with reference to the position numbering of SEQ ID NO: 9.