Patent ID: 8280711
Filing Date: 2012-10-02
Classification: C07K,G16B

Abstract:
1. A method of identifying one or more framework amino acid positions for mutation in a single chain antibody (scFv), the scFv having VH and VL amino acid sequences, the method comprising: a) providing a first database of VH, VL, or VH and VL amino acid sequences, the database being stored in a searchable, fixed medium stored on a computer; b) providing a second database of scFv antibody VH, VL, or VH and VL amino acid sequences selected as having at least one desirable functional property, the database being stored in a searchable, fixed medium stored on a computer; c) determining amino acid variability at each framework position of the first database and at each framework position of the second database; d) identifying one or more framework positions at which degree of amino acid variability differs between the first database and the second database to thereby identify one or more framework amino acid positions for mutation in a single chain antibody (scFv).