Patent ID: 9182467
Filing Date: 2015-11-10
Classification: G01N,G01R

Abstract:
1. A method of measuring differences in relative conformations of a recombinant glycoprotein provided in different preparations, comprising steps of: generating a first 2D NOESY NMR spectrum of a first preparation of a recombinant glycoprotein using an NMR spectrometer, wherein the recombinant glycoprotein has been recombinantly expressed in a mammalian cell; generating a second 2D NOESY NMR spectrum, comparable to the first spectrum, of a second preparation of the same recombinant glycoprotein using an NMR spectrometer, wherein the recombinant gycoprotein has been recombinantly expressed in a mammalian cell; and measuring differences in conformation of the recombinant glycoprotein in the first and second glycoprotein preparations by comparing one or more cross-peaks in the first 2D NOESY NMR spectrum with one or more corresponding cross-peaks in the second 2D NOESY NMR spectrum.