Patent ID: 6685811
Filing Date: 2004-02-03
Classification: C07K,G01N

Abstract:
A method, suitable for automation, for separation, especially preparative separation, of amphoteric macromolecules, comprising the steps of:(a) subjecting a mixture of amphoteric macromolecules to isoelectric focusing in liquid media; (b) collecting samples from step (a), said samples containing the macromolecules separated on basis of isoelectric point, and transferring each sample to a channel (13) accommodating medium for electrophoresis, said channel being part of a composite body (14); (c) subjecting said samples, contained in each of the channels (13) in said composite body (14), to electrophoresis; and (d) allowing electrophoresis to proceed until sample fractions in each channel resulting from the electrophoresis are eluted from the medium for electrophoresis, and collecting the eluted sample fractions.