Patent ID: 6989276
Filing Date: 2006-01-24
Classification: G01N,Y10S

Abstract:
1. A method for detecting a selected drug in a biological sample comprising individual specific antibodies and identifying a source of the biological sample, the method comprising: immobilizing multiple antigens in a pre-selected pattern on a solid support; immobilizing a detectable amount of a selected drug on the solid support, to form an array; providing an antibody-enzyme conjugate comprising an antibody configured to bind the selected drug and an enzyme that is capable of converting a colorigenic substrate into a colored product; contacting the array with a biological sample, to bind at least some of the multiple antigens with individual specific antibodies in the biological sample, to form immune complexes; contacting the array with the antibody-enzyme conjugate, wherein the antibody-enzyme conjugate competitively binds to (i) the selected drug immobilized on the array, to form an immobilized antibody-enzyme conjugate, and (ii) any selected drug that may be present in the biological sample, to form a soluble drug-antibody-enzyme conjugate; washing the solid support, to remove at least the soluble drug-antibody-enzyme complexes; contacting the solid support with a colorigenic substrate to convert the colorigenic substrate to a colored product using the immobilized antibody-enzyme conjugate; determining an amount of the colored product present, wherein the amount of the colored product may be inversely correlated with an amount of the selected drug in the biological sample; and detecting the immune complexes immobilized on the solid support to form an antibody profile characteristic of the source of the biological sample.