Patent ID: 6509153
Filing Date: 2003-01-21
Classification: C12N,C12Q

Abstract:
A method of analysis of the toxic potential, in a human or murine cell, of a test compound, said method comprising:(a) contacting, under conditions allowing hybridization to occur, (i) a nucleic acid sample from human or murine cells treated with a candidate therapeutic molecule selected from a peptide, a polypeptide, a nucleic acid, a lipid, a carbohydrate, a chemical molecule and a plant extract and (ii) a library of nucleic acids, wherein said library comprises, immobilized on a support, nucleic acid molecules comprising a sequence specific for genes or for splicing forms of genes, the expression of said genes or splicing forms being induced in a human or murine cell having a deregulated p53 gene expression or a deregulated p53 cell signalling pathway, and (b) analysing the hybridization profile between said nucleic acid sample and said library to determine the toxic potential of the candidate therapeutic molecule.