Patent ID: 6376207
Filing Date: 2002-04-23
Classification: C07K,Y10S

Abstract:
A method of quantifying hBNP in a biological fluid which method comprises forming a labeled complex said complex consisting of(a) a first antibody selected from the group consisting of: (i) an antibody that is monospecifically reactive to an hBNP fragment hBNP 5-13 (positions 2-10 of SEQ. ID. NO: 2) or a fragment of said antibody that is monospecifically reactive to said hBNP fragment hBNP 5-13; (ii) an antibody that is monospecifically reactive to an hBNP fragment hBNP 1-10 (positions 1-10 of SEQ. ID. NO: 3) or a fragment of said antibody that is monospecifically reactive to said hBNP fragment hBNP 1-10; (iii) an antibody that is monospecifically reactive to an hBNP fragment hBNP 15-25 (positions 2-12 of SEQ. ID. NO: 4) or a fragment of said antibody that is monospecifically reactive to said hBNP fragment hBNP 15-25; (b) any hBNP contained in said biological fluid; (c) a second antibody immunoreactive with hBNP or a fragment of said antibody that is immunoreactive with hBNP, and (d) at least one label; and determining the amount of hBNP in the sample by quantifying the label in said complex.