Patent ID: 7232657
Filing Date: 2007-06-19
Classification: C12Q

Abstract:
1. A method of evaluating the efficacy of a treatment for modulating or stopping replication of HIV in a subject, the method comprising: a) administering a treatment to a subject; b) obtaining a biological sample from the subject, c) selectively purifying extrachromosomal DNA comprising an HIV 2-LTR circle from the sample, d) specifically amplifying a region of 2-LTR circular genome comprising a sequence, wherein a mutation of said sequence confers drug resistance, and e) detecting a drug-resistance mutation in the amplified region of the HIV 2-LTR circle, wherein the presence or absence of a drug-resistance mutation in the HIV 2-LTR circle is indicative of the efficacy of the treatment for modulating or stopping replication of HIV in the subject.