Patent ID: 6331527
Filing Date: 2001-12-18
Classification: A01K,A61K,A61P,C07K,C12N

Abstract:
A method for providing a molecule of interest to a blood vessel in a mammal comprising:(a) providing a bioprosthesis seeded with a smooth muscle cell transfected with a nucleic acid segment comprising an SM22.alpha. promoter region operably linked to a DNA sequence encoding said molecule of interest that is other than mouse SM22.alpha., wherein the SM22.alpha. promoter region has a maximum of 441 bases and comprises:(i) bases 900-1340 of SEQ ID NO:1;(ii) a sequence hybridizing with the complement of SEQ ID NO:1 under hybridizing conditions comprising 0.02M-0.15M sodium chloride at a temperature of 50.degree. C. to 70.degree. C.;(iii) an oligomer comprising SME1 (SEQ ID NO:21), SME2 (SEQ ID NO:23), SME3 (SEQ ID NO:25), SME4 (SEQ ID NO:27), SME5 (SEQ ID NO:31), or SME6 (SEQ ID NO:33); or(iv) a sequence that hybridizes with the complement of SME1 (SEQ ID NO:21), SME2 (SEQ ID NO:23), SME3 (SEQ ID NO:25), SME4 (SEQ ID NO:27), SME5 (SEQ ID NO:31), or SME6 (SEQ ID NO:33) under hybridizing conditions comprising 0.02M-0.15M sodium chloride at a temperature of 50.degree. C. to 70.degree. C.;(b) placing said seeded prosthesis in said blood vessel; and(c) obtaining expression of said molecule in said blood vessel.