Patent ID: 6740734
Filing Date: 2004-05-25
Classification: C07K

Abstract:
A method for selecting an artificial bacterial receptor structure, the method comprising:producing an expression library that produces a protein comprising an artificial bacterial receptor structure, wherein the amino acid sequence of the artificial bacterial receptor structure corresponds to that of a natural bacterial receptor having at least one surface-exposed amino acid residue substituted by another amino acid residue, wherein the basic structure and stability of the natural bacterial receptor is not lost, wherein the artificial bacterial receptor structure lacks an interaction capacity of the natural bacterial receptor, and wherein the artificial bacterial receptor structure binds to an interaction partner to which the natural bacterial receptor does not bind; screening the expression library with a putative binding partner that is not bound by the natural bacterial receptor; detecting specific binding of the artificial bacterial receptor structure to the putative binding partner; and selecting a DNA from the expression library that encodes the artificial bacterial receptor structure that specifically binds to the putative binding partner.