Patent ID: 6808915
Filing Date: 2004-10-26
Classification: C11D,C12N

Abstract:
A variant of a wild-type parent pectate lyase (EC 4.2.2.2) having the conserved amino acid residues D111, W123, D125, H126, D141 or E141, D145, K165, R194 and R199 when aligned with a pectate lyase comprising the amino acid sequence of SEQ ID NO: 2, which the variant comprises a substitution in at least one position selected from the group consisting of 5, 8, 9, 10, 19, 38, 39, 40, 41, 55, 56, 59, 61, 64, 66, 71, 72, 82, 83, 90, 100, 102, 109, 112, 114, 117, 129, 133, 136, 137, 139, 142, 144, 160, 163, 164, 166, 167, 168, 169, 171, 173, 179, 189, 192, 197, 198, 200, 203, 207, 214, 220, 222, 224, 230, 232, 236, 237, 238, 244, 246, 261, 262, 264, 265, 266, 269, 278, 282, 283, 284, 285, 288, 289 and 297, wherein the variant has pectate lyase activity.