Patent ID: 9206463
Filing Date: 2015-12-08
Classification: C07D,C12Q,G01N

Abstract:
1. A method of testing a disinfectant or antibiotic for the ability to kill a cell, comprising: a) exposing a cell culture to a candidate disinfectant or antibiotic; b) further exposing said cell culture to a phenanthridium derivative of formula (II): wherein R R R R or a mono or bis halide salt thereof, wherein the phenanthridium derivative has an overall charge of 2 c) exposing the phenanthridium derivative containing cell culture to a light source; d) optionally isolating genomic DNA from said cell culture; e) performing PCR on said cell culture or isolated DNA; and f) comparing PCR results between said disinfectant or antibiotic treated cell culture with an untreated cell culture, wherein a lower amount of amplified DNA resultant from said PCR reaction conducted with the cell culture or DNA isolated therefrom that was exposed to the disinfectant or antibiotic, as compared to the amount of amplified DNA resultant from said PCR reaction conducted with the untreated cell culture or DNA isolated therefrom, is indicative of the ability of the disinfectant or antibiotic to kill a cell.