Patent ID: 8932843
Filing Date: 2015-01-13
Classification: B01D,B01J,C07K,C12M

Abstract:
1. A method of harvesting proteins in a bioreactor comprising: a. Providing the protein-harvesting device comprising a porous container with an inner volume comprising a suitable amount of a chromatography media wherein said media is capable of binding a protein; and a buoyancy device attached to the porous container; b. Disposing the protein-harvesting device in a container comprising a genetically modified organism or cells capable of producing a protein for harvesting in a nutrient media; c. Adjusting the buoyancy of the protein-harvesting device to a pre-determined level; d. Adjusting the pH and electrolyte concentration of the nutrient media to optimize the binding of the protein to the chromatography media; e. Testing the nutrient media until the concentration of the protein in the nutrient media reaches a pre-determined low value; f. Removing the protein-harvesting device from the nutrient media for further processing of the protein bound to the chromatography media.