Patent ID: 6541258
Filing Date: 2003-04-01
Classification: C12N

Abstract:
A composition useful in preparing an adeno-associated virus (AAV) split-packaging cell useful for high efficiency packaging of a recombinant adeno-associated virus (rAAV) vector, said composition comprising:(i) a polynucleotide comprising an AAV split-cap gene, wherein said split-cap gene is uncoupled from Rep78-specific sequences of an AAV rep gene, wherein p40 promoter of said split-cap gene is replaced with a first heterologous promoter which is operably linked to said split-cap gene; (ii) a polynucleotide comprising an AAV rep78 gene, wherein said rep78 gene is uncoupled from Cap-specific sequences of an AAV cap gene, wherein p5 promoter of said rep78 gene is replaced with a second heterologous promoter which is operably linked to said rep78 gene; and (iii) a polynucleotide comprising an AAV rep52 gene, wherein said rep52 gene is uncoupled from Rep78-specific sequences of an AAV rep gene, wherein p19 promoter of said rep52 gene is replaced with a third heterologous promoter which is operably linked to said rep52 gene; wherein the polynucleotide of (i) and (iii) are a single polynucleotide construct.