Patent ID: 7319520
Filing Date: 2008-01-15
Classification: G01J,G01N,G02B

Abstract:
1. A method for determining illumination and detection parameters in a spectral scanning microscope for data acquisition comprising: generating light from at least one laser that emits light of one wavelength and converting the light of one wavelength into the light comprising a discrete or continuous wavelength region; calculating a band of the illuminating light for each fluorescent dye present in the specimen and calculating a band of a detected light for each fluorescent dye present in the specimen by using the excitation and emission spectra stored in a database; performing data acquisition with the spectral scanning microscope using for each fluorescent dye in the specimen its calculated band of the illuminating light within the wavelength region and its calculated band of a detected light, wherein the emission spectra s wherein the excitation spectra s wherein the mixed excitation matrix M