Patent ID: 6057098
Filing Date: 2000-05-02
Classification: C07K,C12N,C40B

Abstract:
A method of producing a multivalent polypeptide display library, comprising:(a) providing a library of replicable genetic packages, wherein a member comprises a replicable genetic package capable of displaying from its outersurface a fusion protein comprising polypeptide to be screened and a tag, which fusion protein is encoded by a segment of a genome of the package, the polypeptide varying between library members, the number of copies of the fusion protein displayed per library member varying between library members, and the tag being the same in different library members; and(b) contacting the library with a receptor having a specific affinity for the tag under conditions whereby library members displaying at least two copies of the fusion protein are preferentially bound to immobilized receptor by multivalent bonds between the receptor and the at least two copies of the tag;(c) separating library members bound to the immobilized receptor from unbound library members to produce a sublibrary enriched relative to the library for members displaying at least two copies of the fusion protein.