Patent ID: 6368827
Filing Date: 2002-04-09
Classification: C07K,C12N

Abstract:
A method for producing a polypeptide comprising:(a) providing a nonpathogenic protozoan host cell comprising a vector comprising a first 5â€² regulatory region, a first 3â€² regulatory region, and a first coding region located therebetween, wherein the first coding region encodes a polypeptide and is operably linked to the first 5â€² regulatory region and the first 3â€² regulatory region, and a second 5â€² regulatory region, a second 3â€² regulatory region, and a second coding region located therebetween, wherein the second coding region encodes a detectable marker and is operably linked to the second 5â€² regulatory region and the second 3â€² regulatory region; and (b) culturing the host cell under conditions that allow expression of the first coding region to produce the polypeptide.