Patent ID: 6153183
Filing Date: 2000-11-28
Classification: A61K,A61P,C07K

Abstract:
A composition comprising;a modified human interleukin-3 polypeptide, wherein the modification comprises the replacement of 6 to about 44 residues at positions 17-118 of native hIL-3 (1-133) by other amino acids with the proviso that the amino acid at position 101 is Asn and position 116 is Lys, and optionally the deletion of from 1 to 14 amino acids from the N-terminus and/or 1 to 15 amino acids from the C-terminus of said modified human interleukin-3 polypeptide; and wherein said modified human interleukin-3 polypeptide has at least three times greater activity than native human interleukin-3, in at least one assay selected from the group consisting of: AML cell proliferation and TF-1 cell proliferation; anda factor selected from the group consisting of a colony stimulating factor, a cytokine, a lymphokine, an interleukin, and a hematopoietic growth factor.