Patent ID: 6221675
Filing Date: 2001-04-24
Classification: A61K,C07K,C12Q,G01N

Abstract:
A method for the detection of TNF in a biological sample, comprising contacting said sample with an effective amount of a recombinant polypeptide comprising the amino acid sequence,asp ser val cys pro gln gly lys tyr ile his pro gln asn asn ser ile cys cys thr lys cys his lys gly thr tyr leu tyr asn asp cys pro gly pro gly gln asp thr asp cys arg glu cys glu ser gly ser phe thr ala ser glu asn his leu arg his cys leu ser cys ser lys cys arg lys glu met gly gln val glu ile ser ser cys thr val asp arg asp thr val cys gly cys arg lys asn gln tyr arg his tyr trp ser glu asn leu phe gln cys phe asn cys ser leu cys leu asn gly thr val his leu ser cys gln glu lys gln asn thr val cys thr cys his ala gly phe phe leu arg glu asn glu cys val ser cys ser asn cys lys lys ser leu glu cys thr lys leu cys leu pro gln ile glu asnor a fragment, variant, or functional derivative thereof which binds to TNF, and detecting whether a complex is formed.