Patent ID: 6268125
Filing Date: 2001-07-31
Classification: G01N,Y10S

Abstract:
A surface plasmon resonance apparatus for detecting single particulate analytes, the apparatus comprising:(a) a sensor, or means to receive a sensor, said sensor providing a metallised surface capable of binding the analyte;(b) a light source capable of generating an evanescent wave at the sensor surface;(c) a detector capable of detecting light scattered or emitted from a single particulate analyte bound at the sensor surface, said detector being located on the opposite side of the sensor surface to which light from said source is incident;a second light source for increasing the intensity of the light scattered or emitted from an analyte bound to the sensor surface, wherein the second light source is located such that light transmitted therefrom travels along the same light path but in the opposite direction from the light from the first light source which is internally reflected from the sensor surface to the first detector.