Patent ID: 6416734
Filing Date: 2002-07-09
Classification: A61K,C07K,C12N

Abstract:
A method of imaging a neoplastic cell-containing region in a human patient in vivo, said method comprising:(a) providing a detectably-labeled molecule comprising a detectably-labeled recombinant human alpha-fetoprotein (SEQ ID NO: 2) or a detectably-labeled fragment thereof capable of binding to a human neoplastic cell, said fragment being selected from the group consisting of Domain I (residues 1 to 197; SEQ ID NO: 3), Domain II (residues 198 to 389; SEQ ID NO: 4), Domain III (residues 390 to 590; SEQ ID NO: 5), Domain I+II (residues 1 to 389; SEQ ID NO: 6), Domain II+III (residues 198 to 590; SEQ ID NO:7), and rHuAFP Fragment I (residues 266 to 590; SEQ ID NO: 8), wherein said recombinant human alpha fetoprotein or said fragment thereof is produced in a prokaryotic cell and is unglycosylated; (b) administering said molecule to said patient; (c) allowing said labeled molecule to bind and allowing unbound molecule to be cleared from said neoplastic cell-containing region; and (d) obtaining an image of said neoplastic cell-containing region.