Patent ID: 6127143
Filing Date: 2000-10-03
Classification: A61P,C07K

Abstract:
A method for producing purified soluble collagen comprising:a) providing:i) a sample comprising collagen,ii) first and second proteolytic enzyme preparations, wherein said first and second proteolytic enzyme preparations each comprise a proteolytic enzyme in the cysteine class of enzymes;iii) a solubility agent, comprising acetic acid; andiv) a reducing agent;b) exposing said collagen sample to said first proteolytic enzyme preparation to produce a first collagen solution;c) exposing said first collagen solution to said reducing agent to produce a second collagen solution;d) exposing said second collagen solution to said second proteolytic enzyme preparation to provide a third collagen solution; ande) exposing said third collagen solution to said solubility agent to produce soluble purified collagen.