Patent ID: 8675192
Filing Date: 2014-03-18
Classification: G01N

Abstract:
1. A method of high speed quantitative measurement of biomolecular targets present on or in a biological analysis support ( 2 ), comprising: (a) scanning measuring points of the support by moving, focusing and superimposing at least two laser beams (F″) on each measuring point by simultaneous intersection of these beams to extract therefrom a contained hot plasma (P), the plasma comprising: at least one chemical element which is to be quantified and which is present in said targets, and at least one other chemical element which is exogenous to these targets and which is present in a known amount on or in this support, (b) detecting and analyzing light emission lines from each plasma which correspond to the or to each element to be quantified and to the or to each exogenous element for each of these measuring points, by measuring the respective intensities of these lines, and (c) determining the concentration in each measuring point of the or of each element to be quantified or of a group incorporating the element to be quantified within the targets via a prior calibration of the lines of the or of each element to be quantified establishing a correlation between the intensities of the lines specific to this element to be quantified and the concentrations of the element to be quantified in mixtures, in known proportions, of the or of each element to be quantified and of the or of each exogenous element.