Patent ID: 6291155
Filing Date: 2001-09-18
Classification: C12Q,G01N,Y10S

Abstract:
A method of producing an electrode membrane combination comprising:(1) Forming a first solution containing Linker Lipid A, disulfide of mercaptoacetic acid (MAAD)or similar molecule, linker Gramicidin B, membrane spanning lipid C (MSL-C) and membrane spanning lipid D (MSL-D) or other suitable linker molecules and other ion channel or ionophore combinations;(2) Contacting an electrode containing a clean gold surface with the solution to allow the disulfide containing components of said solution being adsorbed onto the gold surface of the electrode;(3) Rinsing the electrode with a suitable organic solvent; and(4) Removing the excess organic solvent used for rinsing;(5) Adding a solution of lipid and biotinylated gramiciden E dispersed in a suitable solvent onto the electrode surface containing the first solution;(6) Rinsing the electrode surface with an aqueous solution;(7) Adding a solution of streptavidin, avidin, neutavidin, avidin or streptovidin derivative;(8) Rinsing the electrode with an aqueous solution to remove excess streptavidin avidin, neutavidin, avidin or streptovidin derivative;(9) Adding a solution of a biotinylated binding partner molecule; and(10) Rinsing the coated electrode with an aqueous solution.