Patent ID: 9079972
Filing Date: 2015-07-14
Classification: C07K,C12Q,G01N

Abstract:
1. A method of screening a substance for improving cellular insulin resistance, comprising the steps of: bringing an adipocyte capable of expressing or secreting a polypeptide having the amino acid sequence of SEQ ID NO: 1 into contact with a candidate substance to stimulate the adipocyte to express or secrete the polypeptide; isolating the polypeptide by solubilizing the adipocyte and extracting the polypeptide; fragmenting the resulting polypeptide having the amino acid sequence of SEQ ID NO: 1 using trypsin; measuring a level of a polypeptide fragment consisting of 13 continuous amino acids 297-309 of SEQ ID NO: 1 in the adipocyte when the candidate substance is brought into contact; and comparing the measured level of the polypeptide fragment consisting of the 13 continuous amino acids 297-309 of SEQ ID NO: 1 in the adipocyte when the candidate substance is brought into contact, with an expression level or a secretion level of the polypeptide in the adipocyte when the candidate substance is not brought into contact, wherein a reduction of the measured level of the polypeptide fragment consisting of the 13 continuous amino acids 297-309 of SEQ ID NO:1 when the candidate substance is brought into contact, compared with the expression level or the secretion level when the candidate substance is not brought into contact, is regarded as one index for selecting the candidate substance as a substance that improves cellular insulin resistance, and wherein the step of bringing the adipocyte capable of expressing or secreting the polypeptide having the amino acid sequence of SEQ ID NO: 1 into contact with a candidate substance is conducted in vitro.