Patent ID: 6713622
Filing Date: 2004-03-30
Classification: C07H

Abstract:
A dye-labeled polynucleotide comprising a polynucleotide covalently attached by a linkage to a 4,7-dichlororhodamine dye, wherein the linkage is attached to a terminus of the polynucleotide, and the 4,7-dichlororhodamine dye comprises the structures: whereinR7, R8, R9, R10, R12, R13, R14, R15, R16, and R18 are each, independently of one another, selected from hydrogen, fluorine, chlorine, methyl, ethyl, lower alkyl, lower alkene, lower alkyne, cycloalkyl, phenyl, aryl, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy and a linkage, or, alternatively, R7, and R8, and/or R13 and R14 may be taken together to form an oxo, thioxo, imminium or alkylimminium group; R11 and R17 are each, independently of one another, selected from hydrogen, lower alkyne, alky sulfonate, alkyl carboxylate, lower alkene, lower alkyne, cycloalkyl, phenyl, aryl, and a linkage; and X1, X2 and X3 are each, independently of one another, selected from hydrogen, chlorine, fluorine, lower alkyl, amine, carboxylate, sulfonate, hydroxymethyl and a linkage.