Patent ID: 6653077
Filing Date: 2003-11-25
Classification: C12Q

Abstract:
A method of identifying polymorphic DNA sequences in a test DNA population, the method comprising the steps of:(a) providing a reference DNA population; (b) forming a population of heteroduplexes from single stranded DNA of the reference DNA population and single stranded DNA of the test DNA population, said heteroduplexes having at one terminus a region in which both strands are in single stranded form; (c) treating said heteroduplexes with an exonuclease whose substrate is duplex DNA, such that said exonuclease converts substantially every perfectly matched duplex to single stranded DNA and substantially every mismatched duplex to partially double stranded DNA; (d) extending said partially double stranded DNA to form double stranded heteroduplexes; (e) amplifying the double stranded heteroduplexes to form a population of amplicons; and (f) determining the nucleotide sequence of a portion of each amplicon so that polymorphic DNA sequences of the test DNA population are identified.