Patent ID: 6015695
Filing Date: 2000-01-18
Classification: A61K,A61P,C07K,Y10S

Abstract:
An immunoglobulin comprising two heavy polypeptide chains, each heavy chain consisting of a complete antigen binding site, said immunoglobulin containing a variable (V.sub.HH) region and a constant region, said constant region being devoid of first constant domain C.sub.H 1, wherein the immunoglobulin is devoid of polypeptide light chains, wherein said immunoglobulin is obtainable by a process comprising the steps of:obtaining a first DNA or cDNA sequence coding for a V.sub.HH domain or part thereof having a determined specificity against a given antigen and comprised between Xho and Spe sites, obtaining a second DNA or cDNA sequence coding for a V.sub.HH domain or part thereof, having a determined specificity different from the specificity of the first DNA or cDNA sequence and comprised between the Spe and EcoRI sites;digesting an immuno PBS vector with EcoRI and XhoI restriction enzymes, ligating the obtained DNA or cDNA sequence coding for V.sub.HH domains so that the DNA or cDNA sequences are serially cloned in the vector;transforming a host cell by transfection; andrecovering the immunoglobulins.