Patent ID: 7273933
Filing Date: 2007-09-25
Classification: C07H

Abstract:
1. A method for the preparation of a linear phosphorus-linked oligomer comprising the steps of: (a) providing a solid support; (b) attaching a 5′-O-protected nucleoside to the solid support; (c) deprotecting the 5′-hydroxyl of the nucleoside with a deprotecting reagent comprising a protic acid in a solvent to deprotect the 5′-hydroxyl of the nucleoside, wherein the solvent consists essentially of an aromatic solvent, an alkyl aromatic solvent, a halogenated aromatic solvent, a halogenated alkyl aromatic solvent, or an aromatic ether solvent; (d) reacting the deprotected 5′-hydroxyl with an 5′-protected activated phosphorus compound to produce a covalent linkage therebetween; (e) oxidizing or sulfurizing the covalent linkage to form a phosphodiester, phosphorothioate, phosphorodithioate or H-phosphonate linkage; (f) repeating steps c through e at least once for subsequent couplings of additional activated phosphorus compounds, to produce the completed phosphorus-linked oligomer; and (g) cleaving the oligomer from the solid support; wherein steps (b) through (f) are performed with an automated device; wherein said oligomer is a linear oligomer.