Patent ID: 8043809
Filing Date: 2011-10-25
Classification: C12Q,Y10S

Abstract:
1. An oligonucleotide primer composition for the detection of at least one mutation relating to a HIV-1 protein of reverse transcriptase by hybridizing with a nucleic acid encoding the HIV-1 protein of reverse transcriptase, wherein the at least one mutation is selected from the group consisting of 103N, 184V, 41L, 65R, 67N, 69T, 70R, 181C, 215T, 215Y, 215F, 215S, 215C, and 215D, comprising: a primer selective for the 41L mutation that is SEQ ID NO: 62, SEQ ID NO: 63, or SEQ ID NO: 64; and at least one of: a primer selective for the 103N mutation, the primer selected from the group of: SEQ ID NOs: 22, 23, 24, 25, 59, 60, and 61; a primer selective for the 184V mutation, the primer selected from the group of: SEQ ID NOs: 33, 34, 35, 88, 89, 102, 103, 104, and 36; a primer selective for the 41 L mutation, the primer selected from the group of: SEQ ID NOs: 65, 96, and 97; a primer selective for the 65R mutation, the primer selected from the group of: SEQ ID NOs: 10, and 98; a primer selective for the 67N mutation, the primer selected from the group of: SEQ ID NOs: 69, and 70; a primer selective for the 69T mutation, the primer selected from the group of: SEQ ID NOs: 12, 13, and 71; a primer selective for the 70R mutations, the primer selected from the group of: SEQ ID NOs: 2, 16, 17, 18, 19, and 100; a primer selective for the 181C mutation, the primer selected from the group of: SEQ ID NOs: 28, 29, 83, and 84; a primer selective for the 215T mutation, the primer selected from the group of: SEQ ID NOs: 38, 39, 74, 75, and 101; a primer selective for the 215Y mutation, the primer selected from the group of SEQ ID NOs: 40; a primer selective for the 215F mutation, the primer selected from the group of SEQ ID NOs: 41; a primer selective for the 215S mutation, the primer selected from the group of SEQ ID NOs: 42; a primer selective for the 215C mutation, the primer selected from the group of SEQ ID NOs: 43; and a primer selective for the 215D mutation, the primer selected from the group SEQ ID NOs: 44.