Patent ID: 8914241
Filing Date: 2014-12-16
Classification: C12Q,G16B

Abstract:
1. A method for determining nucleotide sequences for a plurality of nucleic acids, comprising: (a) performing a cycle of a sequencing procedure for an array comprising a plurality of nucleic acids, wherein the cycle comprises directing radiation from a laser light source to the array at an exposure level and detecting fluorescence emission from the array at the exposure level; (b) increasing the exposure level of the radiation directed to the array to an increased exposure level that is higher than the exposure level; (c) performing another cycle of the sequencing procedure for the array comprising the plurality of nucleic acids, wherein said another cycle comprises directing radiation from the laser light source to the array at the increased exposure level and detecting fluorescence emission from the array at the increased exposure level; and (d) repeating steps (b) and (c) to perform several cycles of the sequencing procedure at an increased exposure level as compared to previous cycles of the sequencing procedure, thereby determining nucleotide sequences for the plurality of nucleic acids.