Patent ID: 7279308
Filing Date: 2007-10-09
Classification: A21D,A23J,A23L,C12N

Abstract:
1. An isolated nucleic acid sequence encoding a secreted polypeptide having aminopeptidase activity with physicochemical properties of (i) a pH optimum in the range of from about pH 7.27 to about pH 10.95 determined at ambient temperature in the presence of Ala-para-nitroanilide; (ii) a temperature stability of 90% or more at pH 7.5 after incubation for 20 minutes at 60° C. in the absence of substrate; (iii) a temperature stability of 64% or more at pH 7.5 after incubation for 20 minutes at 70° C. in the absence of substrate; and (iv) an ability to hydrolyze a substrate containing Ala, Arg, Asn, Asp, Cys, Gln, Glu, Gly, His, lle, Leu, Lys, Phe, Pro, Ser, Thr, Trp, Tyr, or Val at its N-terminus, wherein the isolated nucleic acid sequence is: (a) an isolated nucleic acid sequence encoding a polypeptide having an amino acid sequence which has at least 90% identity with the amino acid sequence of amino acids 16 to 496 of SEQ ID NO:2; or (b) an isolated nucleic acid sequence which hybridizes under medium stringency conditions with (i) the nucleic acid sequence of nucleotides 46 to 1488 of SEQ ID NO:1, or (ii) its full complementary strand, wherein medium stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 35% formamide