Patent ID: 7115371
Filing Date: 2006-10-03
Classification: C07K,G01N

Abstract:
1. A method for detecting and quantifying multiple immunogens in a sample comprising: (a) forming an immunogen-binding agent-DNA complex extended with two oligonucleotides, oligo 1 and oligo 2, by: (i) contacting an immobilized form of immunogens with an epitope detector comprising an immunogen-binding agent-DNA complex, wherein the DNA of the complex contains an RNA polymerase promoter; (ii) incubating the immobilized form of immunogens and the epitope detector under conditions which promote binding of the first immunogen-binding agent-DNA complex to an immunogen; (iii) ligating oligo 1 to the bound first immunogen-binding agent-DNA complex to form a complex immunogen-binding agent-DNA-oligo 1; (iv) ligating oligo 2 to the bound immunogen-binding agent-DNA-oligo 1 complex to form immunogen-binding agent-DNA-oligo 1-oligo 2; (b) repeating step (a) with different epitope detectors comprising Immunogen-binding-agents for different immunogens in the sample; (C) removing all bound immunogen-binding agent-DNA-oligo complexes from the immobilized immunogens; (d) adding RNA polymerase to the immunogen-binding agent-DNA-oligo complexes to form RNA products; and (e) analyzing RNA products wherein an amount of RNA product is directly proportional to the amount of immunogen-binding agent-DNA-oligo complexes bound to the immobilized immunogens in step (a) and size of an RNA product corresponds to the step at which the immunogen-binding agent was added with earlier added immunogen-binding agents giving rise to longer products while later added immunogen-binding agents produce smaller products.