Patent ID: 7419791
Filing Date: 2008-09-02
Classification: C07K,G01N

Abstract:
1. A method for identifying a compound that modulates sweet or umami taste elicited by a receptor comprising a T1R3 polypeptide sequence comprising: (1) screening one or more compounds in a binding assay which identifies compounds that specifically bind to a receptor comprising a T1R3 polypeptide that is at least 90% identical to the polypeptide contained in SEQ ID NO:6 or which specifically modulate (enhances or inhibits) the specific binding of another compound to said receptor comprising said T1R3 polypeptide; and (2) identifying whether said compound potentially modulates umami or sweet taste elicited by a taste receptor comprising a polypeptide having a sequence which is at least 90% identical to the T1R3 polypeptide contained in SEQ ID NO:6 based on its (a) specific binding to a T1R3 receptor or (b) modulation of the specific binding of another compound to a T1R3 receptor, wherein said T1R3 polypeptide possesses at least 90% sequence identity to the T1R3 polypeptide of SEQ. ID. NO: 6 and wherein said T1R3 receptor specifically binds to a ligand that specifically binds to an endogenous (wild-type) human T1R3 receptor and (3) effecting a taste test to confirm whether said identified compound modulates sweet or umami taste.