Patent ID: 7771966
Filing Date: 2010-08-10
Classification: C07K,C12P

Abstract:
1. A process for producing a polypeptide comprising: (a) expressing a recombinant chimeric protein from an expression vector in a host cell, wherein the chimeric protein comprises a leader sequence joined by a Cys-His cleavage site to the N-terminus of the polypeptide and recovering the chimeric protein from the host cell in the form of an inclusion body and wherein the chimeric protein comprises a vestigal sequence of the amino acids 15-41 of SEQ ID NO:15 to induce formation of the inclusion body; (b) cleaving the polypeptide from the chimeric protein by solubilizing the inclusion body recovered in step (a) in a reaction mixture comprised of a palladium promoter dissolved in a high-concentration organic solvent selected from the group consisting of acetic acid, citric acid, dicarboxylic acid, lactic acid, maleic acid, malonic acid, propionic acid, pyruvic acid, tartaric acid, and tricarballylic acid, wherein the concentration of the organic solvent in the reaction mixture is between 1 to 22 molar; and (c) recovering the cleaved polypeptide from the reaction mixture of step (b) by ultrafiltration, filtration or ion-exchange chromatography.