Patent ID: 6936437
Filing Date: 2005-08-30
Classification: C07K,C12P

Abstract:
1. A method for solubilizing and recovering, in bioactive form, a target polypeptide from a host organism in which the target polypeptide is present in insoluble form, which comprises: disrupting the host cell to produce a lysate; recovering lysate precipitate containing the target polypeptide; solubilizing the lysate precipitate in a denaturant free, non-buffered solubilization solution producing a solubilization preparation that comprises 1) a concentration of sodium hydroxide between about 8 and about 10 mM and 2) a concentration of polypeptide between about 1 and about 4 mg polypeptide per ml of solubilization solution, wherein the resultant solubilization preparation has a pH of between about 9 and about 11.2.