Patent ID: 9187759
Filing Date: 2015-11-17
Classification: A01H,A24B,C12N,C12Q

Abstract:
1. Cured tobacco material or a tobacco product comprising said cured tobacco material, wherein said cured tobacco material is made from a tobacco plant comprising a stable mutation in a wild type allele encoding a nicotine demethylase having an amino acid sequence of SEQ ID NO:4 or a sequence having 98% identity to SEQ ID NO:4, wherein said mutation reduces or eliminates the expression or activity of said nicotine demethylase, wherein the amount of nornicotine or N-nitrosonornicotine in said cured tobacco material is reduced by greater than about 10% compared to cured tobacco material made from a control tobacco plant comprising said wild type allele; and wherein said tobacco plant is a Nicotiana tabacum plant.