Patent ID: 9052322
Filing Date: 2015-06-09
Classification: C07K,C12Q,G01N

Abstract:
1. A method for identifying an antibody that binds to an antigen, comprising: a) obtaining: (i) antibody heavy chain variable domain sequences and (ii) antibody light chain variable domain sequences by sequencing heavy and light chain-encoding cDNAs made from a population of antibody-producing B cells of an animal that has been immunized by the antigen; b) grouping the heavy chain variable domain sequences of step a) into a plurality of heavy chain groups and, independently, grouping the light chain variable domain sequences of step a) into a plurality of light chain groups, wherein: c) selecting a heavy chain group and a light chain group from the groups produced by step b); d) pairing a heavy chain variable domain sequence and a light chain variable domain sequence from the groups selected in c); and e) testing an antibody comprising the heavy and light chain sequences paired in d) for binding to the antigen.