Patent ID: 8766631
Filing Date: 2014-07-01
Classification: G01R

Abstract:
1. A method for carrying out an NMR experiment with enhanced sensitivity on a compound comprising hyperpolarizable nuclei using a device for repeated preparation of a nuclear spin resonance (NMR) liquid measurement sample in a mixing chamber and for measurement of the sample in a flow-through NMR probehead of an NMR measurement apparatus, the device comprising: a mixing chamber having means for mixing the liquid measurement sample, contained in said mixing chamber, with a gas; a flow-through NMR probehead; a first tube and a second tube for supply and removal of the liquid NMR measurement sample from said mixing chamber to and from said flow-through NMR probehead; a sample inlet for introducing the liquid measurement sample into the device; at least one connection for introducing the gas, for increasing and for releasing gas pressure in said mixing chamber; and a reservoir structured and dimensioned for storing para-hydrogen, wherein said connection for introducing the gas and for increasing gas pressure leads to said reservoir for para-hydrogen; the method comprising the steps of: a) preparing a fluid having a temperature TF, containing spatially symmetric molecules comprising two halves each, with a non-Boltzmann nuclear spin state distribution of the symmetric molecules at this temperature TF, wherein the symmetric molecules are para-hydrogen molecules; b) providing the compound with a defined chemical identity; c) providing a template that offers sites of ordered environment for the two halves of one of the symmetric molecules and the compound which can be arranged at each site, wherein the ordered environment distinguishes chemically or magnetically the two halves of one of the symmetric molecules arranged at each site, the ordered environment allowing interaction, via scalar or dipolar coupling, between the two halves of one of the symmetric molecules and the compound arranged at each site; d) bringing together, in said mixing chamber or in a second chamber, the prepared fluid, the provided compound and the provided template, thereby transferring spin order from the symmetric molecules to the hyperpolarizable nuclei of the compound during a temporary association of the symmetric molecules, the compound, and the template while ultimately keeping the chemical identity of the compound; e) transporting, following step d), at least part of the compound into the flow-through NMR probehead, via one of the first and the second tubes; f) performing, following step e), an NMR measurement on the part of the compound present in the flow-through NMR probehead comprising hyperpolarized nuclei prepared in step d); and g) transporting, following step f), the part of the compound present in the flow-through NMR probehead into the mixing chamber or the second chamber of the device, thereby permitting renewed transfer of spin order to the hyperpolarizable nuclei in the part of the compound and renewed NMR measurement of those hyperpolarized nuclei.