Patent ID: 7192767
Filing Date: 2007-03-20
Classification: B01J,C07K,C40B

Abstract:
1. A process for carrying out a coupled in vitro transcription and translation reaction to produce proteins, said method comprising the steps of: providing a reactor, said reactor comprising walls that define a total reactor volume V contacting said reactor with a supply solution, said supply solution comprising each of the 20 naturally occurring amino acids, ATP, GTP, CTP, and UTP, wherein said components of the supply solution have a molecular weight smaller than the pores of said matrix and are taken up into the matrix volume V contacting said reactor with a reaction solution, said reaction solution comprising tRNAs, DNA templates, RNA polymerases, mRNA, and enzymes, wherein said tRNAs, DNA templates, RNA polymerases, mRNA, and enzymes each have a molecular weight larger than the pores of said matrix and are only localized in the matrix exclusion volume V moving the reaction solution through the matrix exclusion volume V obtaining said protein by recovering an eluate from the reactor.