Patent ID: 6686162
Filing Date: 2004-02-03
Classification: C12Q,Y02A

Abstract:
A method for producing an oligonucleotide that is a hybrid of human placental DNA with FlaA nucleic acid sequence, comprising:amplifying human placental DNA using a pair of oligonucleotide primers having the sequences set forth in SEQ ID NO:4 and SEQ ID NO:5, wherein said primers each further contain a terminal FlaA nucleic acid sequence comprising 5 or more contiguous nucleotides selected from 5â€²-TTG CAA ATC TTT TCT CTG GTG-3â€² (SEQ ID NO:1) or 5â€²-AGA ATT AAC TCC GCC TTG AGA-3â€² (SEQ ID NO:2) to provide a plurality of human placental-FlaA nucleic acid hybrid amplicons; and purifyng the human placental-FlaA nucleic acid hybrid amplicons.