Patent ID: 8338665
Filing Date: 2012-12-25
Classification: C12N

Abstract:
1. A nucleic acid molecule comprising a first DNA segment comprising a first transcription unit flanked on both sides by a first and a second shorter transcribable nucleic acid sequence in opposite orientation to each other and located between a first left T-DNA border region and a first right T-DNA border region, wherein said first and second shorter transcribable nucleic acid sequences are physically linked to said first border regions and said first T-DNA border regions flank said first DNA segment; and a second DNA segment comprising a second transcription unit flanked on both sides by a third and fourth shorter transcribable nucleic acid sequence in opposite orientation to each other and located between a second left T-DNA border region and a second right T-DNA border region, wherein said third and fourth shorter transcribable nucleic acid sequences are physically linked to said second T-DNA border regions and said second T-DNA border regions flank said second DNA segment, and said third shorter transcribable nucleic sequence is operably linked to a plant-expressible promoter; wherein said first, second, third and fourth shorter transcribable nucleic acid sequences are each at least 100 contiguous nucleotides in length, and share greater than 90% sequence identity to each other and to a portion of said second transcription unit; and wherein said first and second DNA segments are not identical.