Patent ID: 8110348
Filing Date: 2012-02-07
Classification: G01N

Abstract:
1. A method for identifying a compound which inhibits complex formation between nitric oxide synthetase 1 adaptor protein (NOS1AP) and NOS1AP binding protein(s), comprising: a) incubating said NOS1AP or functional fragment thereof and said NOS1AP binding protein in the presence and absence of said compound under conditions suitable for protein complex formation to occur, wherein said NOS1AP or functional fragment thereof is SEQ ID NO: 2, a fragment consisting of amino acids 181-307 of SEQ ID NO: 2, or a fragment consisting of amino acids 186-312 of SEQ ID NO: 2, and b) determining whether the complex formation is decreased in the presence of said compound when compared to complex formation in the absence of said compound, wherein said nitric oxide synthetase 1 adaptor protein (NOS1AP) binding protein is selected from the group consisting of carboxypeptidase E (CPE), eEF2, and hippocalcin-like-1, and wherein a decrease in NOS1AP-NOS1AP binding protein complex formation in the presence of said compound indicates that the compound is an inhibitor of NOS1AP-NOS1AP binding protein complex formation.