Patent ID: 8815594
Filing Date: 2014-08-26
Classification: A61L,C12N

Abstract:
1. A method of forming a hybrid tissue scaffold comprising: forming a solution of biodegradable polymer in the presence of an inorganic salt wherein the inorganic salt is water soluble and has a particulate size in a range of 0.50 to 5.0 mm; cooling said solution to precipitate said biodegradable polymer followed by treatment with water to dissolve said inorganic salt wherein the biodegradable polymer is recovered as a porous primary scaffold having a pore size in a range of 1 mm to 4 mm; introducing into said primary scaffold a biomaterial comprising a hydrogel including living cells and forming a secondary scaffold from said biomaterial including living cells, wherein the secondary scaffold resides in the pores of the primary scaffold to provide a hybrid scaffold; wherein said biomaterial provides an environment to culture the living cells and said biomaterial has a pore size of 50 μm to 600 μm; and wherein said primary scaffold is present at 5.0% to 30.0% by volume of said hybrid scaffold and said hybrid scaffold has a Young's modulus of 0.1 MPA to 100 GPa.