Patent ID: 6570068
Filing Date: 2003-05-27
Classification: C12N

Abstract:
A method of transforming maize tissue with exogenous DNA, comprising:precipitating said DNA onto microprojectiles of about 0.7-1.8 micron average size in an amount corresponding to a ratio of about 0.75-1.25 micrograms DNA per 720 micrograms of microprojectile, bombarding nodal explants of maize that form adventitious shoots which are approximately 3-10 days old with said DNA-bearing microprojectiles by accelerating said DNA-microprojectiles at a pressure of about 625-1350 psi, said microprojectiles being expelled from a source developing said pressure upstream from said microprojectiles wherein said nodal explants are at least 7.5 cm from said microprojetiles, prior to acceleration.