Patent ID: 9138487
Filing Date: 2015-09-22
Classification: A23L,A61K,A61P,A61Q

Abstract:
1. A method for preparing plasma membrane vesicles enriched in intrinsic membrane proteins comprising: (a) irrigating a plant from the genus (b) modifying electrical conductivity of the mixture solution from 0.5 to 6 dS m (c) adding 5-100 μM glucosinolate sinigrin as a bioactive compound to the mixture solution and incubating for 1-78 hours; (d) extracting the membrane vesicles by homogenization of the mixture solution, re-suspending in a buffer solution and centrifuging to separate membrane vesicles into an upper phase; (e) collecting the upper phase containing the membrane vesicles and re-suspending the collected upper phase in a storage buffer selected a group consisting of a phosphate buffer, a bicarbonate buffer and an acetate buffer.