Patent ID: 6103472
Filing Date: 2000-08-15
Classification: C12N

Abstract:
A method for identifying a novel secreted mammalian protein comprising the steps of:a) constructing a mammalian cDNA library;b) inserting the cDNA library of step (a) into a signal trap vector to generate a signal trap library, wherein the vector comprises DNA encoding .alpha.-amylase lacking a functional signal sequence;c) amplifying the signal trap library of step (b);d) transforming the library of step (c) into a yeast host cell lacking a functional gene encoding .alpha.-amylase;e) selecting transformed yeast cells from step (d) for utilization of starch in growth medium;f) analyzing the DNA recovered from the transformed yeast cells of step (e) to determine whether a functional mammalian signal sequence is present; andg) screening a mammalian cDNA library to identify a full-length cDNA comprising the functional mammalian signal sequence of step (f).