Patent ID: 7226738
Filing Date: 2007-06-05
Classification: C12Q

Abstract:
1. A method of detecting multiple reiterated oligonucleotides from a target DNA or RNA polynucleotide in a biological or environmental sample, said method comprising: (a) incubating said target polynucleotide with an initiator, a target site probe, an RNA-polymerase, and a terminator, under conditions whereby said initiator, wherein said target site probe and said target polynucleotide form a bubble complex comprising a first double-stranded region upstream of said target site, a single-stranded region comprising said target site, and a second double-stranded region downstream of said target site; (b) extending said initiator until said terminator is incorporated into said oligonucleotide; (c) repeating steps (a) and (b), thereby synthesizing multiple reiterative oligonucleotide transcripts from said target polynucleotide; and (d) detecting or quantifying said reiterative oligonucleotide transcripts.