Patent ID: 8778647
Filing Date: 2014-07-15
Classification: C12P,Y02E

Abstract:
1. A method of producing isoprene, the method comprising: (a) culturing cells comprising a heterologous nucleic acid encoding an isoprene synthase polypeptide under suitable culture conditions for the production of isoprene; (b) removing a portion of the culture; (c) filtering the removed portion of the culture to produce a permeate and a retentate; (d) returning the retentate to the culture; and (e) producing isoprene; wherein the cultured cells undergoing steps (b), (c), and (d) either produce isoprene at a higher titer, or have greater average volumetric productivity of isoprene than the same cells cultured without undergoing steps (b), (c), and (d).