Patent ID: 7794778
Filing Date: 2010-09-14
Classification: G01N

Abstract:
1. A manufacturing method for an amperometric sensor for uric acid, comprising the steps of: (a) fixing a conducting wire on a carbon electrode with a silver paste and then baking the carbon electrode in an oven, said conducting wire comprising aluminum; (b) packaging the portion of the sensor device containing the conducting wire with epoxy and then baking the sensor in an oven to manufacture a tri-polar carbon electrode; (c) forming the tri-polar carbon electrode having a sensing window thereon; (d) mixing polyacrylamide and a phosphate solution; (e) forming a polyacrylamide solution by mixing the polyacrylamide used in step (d) with ferrocenecarboxylic acid; (f) diluting uricase with the polyacrylamide solution obtained in step (e); and (g) dripping a proper amount of the diluted uricase obtained in step (f) on the sensing window, and distributing the diluted uricase uniformly thereon, and then air-drying the diluted uricase to fix it, and the amperometric sensor for uric acid is achieved.