Patent ID: 6676974
Filing Date: 2004-01-13
Classification: A61K,A61P,Y02A

Abstract:
A process for the isolation of bioactive fraction of the plant Vetivera zizanioides comprising the steps of:a) powdering the plant part of Vetivera zizanioides, b) extracting the plant powder of step (a) by soaking in protic aqueous organic solvent for a period of 16-20 hours, c) filtering the organic solvent extract of step (b), d) evaporating the extract of step (c) under reduced pressure to remove the organic solvent to obtain an aqueous extract, e) lyophilising the aqueous extract of step (d) to get an powdered extract, f) dissolving the powdered extract of step (e) in 2% aqueous citric acid, g) extracting the solution of step (f) successively with chloroform, n-butanol, methanol and finally with acetone to obtain respective organic extracts and an aqueous solution, h) evaporating separately the organic extracts of step (g) to obtain respective residues, i) neutralizing the aqueous solution of step (g) with ammonia solution, j) testing the bioactivity of residues obtained in step (h) and neutralized solution of step (i) to identify residue from methanolic extract as bioactive residue, k) macerating the residue of methanoloic extract of step (h) successively with hexane, chloroform and ethylacetate, l) testing bioactivity of hexane, chloroform ethylacetate fractions of step (k) to identify hexane fractions as a bioactive fraction, m) purifying the residue of hexane faction of step (l) on a silica gel column using eluant hexane and mixture of hexane-chloroform with increasing polarity, and n) evaporating the hexane-choroform (1:1) eluant fraction obtained from step (m) to yield a residue, which is purified by thin layer chromatography to achieve the required bioactive fraction.