Patent ID: 6025127
Filing Date: 2000-02-15
Classification: A61K,C07K,C12N,C12Q

Abstract:
A method for detecting the presence of a mammalian target nucleic acid which contributes to the etiology of a neoplasm, in a tissue specimen, wherein the specimen is external to a primary neoplasm and the specimen does not exibit morphological charactersics indicative of neoplastic pathology, and the target nucleic acid is present in the primary neoplasm and the specimen, the specimen being selected from the group consisting of a tumor margin, a regional lymph node, chyle and blood the method comprising amplifying the nucleic acid present in the specimen by means of oligonucleotides that hybridize to the flanking regions of the target nucleic acid, wherein the nucleotide sequence of the flog region to which the oligonucleotide hybridizes is selected from the group of sequences consisting of:a) 5'-AAGTCAGGGCACAAGTGAATTCCTAC-3', (SEQUENCE ID NO. 1);b) 5'-AAGGGTGGTTGTCAGTGGAATTCGATG-3' (SEQUENCE ID NO. 2);c) 5'-GAGGCCAGTGCGCCTTGGAATTCCTAC-3' (SEQUENCE ID. NO. 3);d) 5'-GCGGTGGAGGAGACGAAGAATTCAGT-3' (SEQUENCE ID. NO. 4); ande) sequences complementary to a) through d) and detecting the presence of the target nucleic acid.