Patent ID: 8658777
Filing Date: 2014-02-25
Classification: C07K,C12N,C12Q,G01N

Abstract:
1. A recombinant indicator for an active protease, which is a circular form comprising a C-half fragment of a click beetle or firefly luciferase (Luc-C), an N-half fragment of firefly luciferase (Luc-N), and a substrate peptide for a protease, wherein the C-terminus of the Luc-N is linked with the N-terminus of the Luc-C, the N-terminus of the Luc-N is linked with the C-terminus of the substrate peptide, and the C-terminus of the Luc-C is linked with the N-terminus of the substrate peptide, wherein the N-half fragment of luciferase consists of amino acids residues 2 to 416 of SEQ ID NO: 5, and the C-half fragment of luciferase consists of of amino acids residues 399 to 550 of SEQ ID NO: 5; wherein the recombinant indicator is non-luminescent when in the circular form, and wherein the Luc-N and the Luc-C reconstructs an active luciferase upon digestion of the substrate peptide by the protease and is luminescent when digested into a non-circular form.