Patent ID: 6342360
Filing Date: 2002-01-29
Classification: A01K,A61K,C07K,C12N,C12Q

Abstract:
A method of screening a compound for binding to a mammalian D2 dopamine receptor on the surface of cells expressing the receptor, the method comprising the following steps:(a) transforming a host cell with a recombinant expression construct encoding a mammalian D2 dopamine receptor having an amino acid sequence depicted in FIGS. 1A through 1E or in FIGS. 18A through 18H or an amino acid sequence depicted in FIGS. 18A through 18H wherein amino acid residues 242 through 270 are deleted therefrom and amino acid 271 is an aspartic acid residue, wherein the cells of the transformed cell culture express the receptor; and (b) assaying the transformed cell with the compound to determine whether the compound binds to the receptor.