Patent ID: 8859954
Filing Date: 2014-10-14
Classification: G01N,Y10T

Abstract:
1. A method for discriminating sitosterolemia comprising the following steps: preparing a piece of paper stained with one drop of blood spot; extracting sitosterol, campesterol and stigmasterol as phytosterols, and cholesterol metabolism-related compounds from the paper; evaluating relative metabolic ratios between the above compounds by using gas chromatography-mass spectrometry; and discriminating the case that relative metabolic ratio of sitosterol/cholesterol is about 0.01 or more, that of campesterol/cholesterol is about 0.02 or more, that of stigmasterol/cholesterol is about 0.001 or more, that of campesterol/sitosterol is about 1.0 or less and that of sitosterol/desmosterol is 100 or more in mass ratio, as sitosterolemia.