Patent ID: 8450087
Filing Date: 2013-05-28
Classification: A01K,A61K,A61P,C07K,C12N

Abstract:
1. A method for generating recombinant lentiviral vector particles comprising transcomplementing a replication-deficient lentiviral vector with Gag, Pol, and Env proteins to generate recombinant lentiviral vector particles comprising the replication-deficient lentiviral vector; wherein the replication-deficient lentiviral vector comprises a transgene, a lentiviral cis-acting central initiation region, and a lentiviral cis-acting termination region, such that a triplex DNA is formed during reverse transcription, wherein the triplex DNA stimulates the nuclear import of the transgene into the nucleus of a cell transduced with said vector; and wherein the replication-deficient lentiviral vector does not encode functional Gag, Pol, and Env proteins.