Patent ID: 8153415
Filing Date: 2012-04-10
Classification: C12N,C12P,C12Y,Y02E,Y02P

Abstract:
1. A recombinant microorganism comprising: (a) an isobutanol producing metabolic pathway, wherein said isobutanol producing metabolic pathway comprises at least one exogenous gene or at least one overexpressed endogenous gene encoding an enzyme that catalyzes a pathway step in the conversion of pyruvate to isobutanol; (b) at least one modification which disrupts the expression or activity of an endogenous enzyme having 3-keto acid reductase activity, wherein said modification is selected from the group consisting of: wherein said endogenous enzyme having 3-keto acid reductase activity catalyzes the conversion of acetolactate to 2,3-dihydroxy-2-methylbutanoic acid (DH2MB); and (c) at least one modification which disrupts the expression or activity of an endogenous enzyme having aldehyde dehydrogenase activity, wherein said modification is selected from the group consisting of: wherein said endogenous enzyme having aldehyde dehydrogenase activity catalyzes the conversion of isobutyraldehyde to isobutyrate.