Patent ID: 6399319
Filing Date: 2002-06-04
Classification: C12Q

Abstract:
A method for identifying potential fungicides which comprises testing a test substance in a protein kinase inhibition assay said assay comprising:a) preparing an assay mixture consisting essentially of a phosphate acceptor, magnesium nitrate, a phosphate donor, NaN3, okadaic acid, and a suitable protein kinase, in the presence of an appropriate solvent, which protein kinase has been isolated from a fungus, and which is active in said assay in the absence of an inhibitor thereto; b) determining the activity of said protein kinase in the presence of: (i) a test substance, (ii) a device for measuring the phosphorylation rates; and c) selecting test substances which inhibit the protein kinase, wherein inhibition of protein kinase activity is indicative of fungicidal activity.