Patent ID: 7378277
Filing Date: 2008-05-27
Classification: A61K,C12N

Abstract:
1. A method for detecting T cell mediated anti-cancer cell activity of a target antigenic peptide, comprising: transforming a hematopoietic stem cell in vitro with a recombinant expression cassette comprising a nucleic acid encoding the target antigenic peptide, and differentiating the transformed stem cell into a transformed dendritic cell; contacting a T cell with the transformed dendritic cell, thereby providing an activated T cell, wherein the T cell and the hematopoietic stem cell are from the same individual; contacting a cancer cell expressing a protein comprising the target antigenic peptide with the activated T cell; and, monitoring the effect of the activated T cell on the cancer cell, thereby detecting the anti cancer cell activity of the target antigenic peptide.