Patent ID: 6562630
Filing Date: 2003-05-13
Classification: G01N,Y10T

Abstract:
A method of preparing preservative-free allergen test solutions in a prepared sterile environment comprising:preparing a sterile environment by utilizing a disinfectant wipe and a sterile barrier field; adding an antigen to a diluent solution; dispersing or suspending the antigen in the diluent to form an initial solution; maintaining the initial solution at a temperature selected to impede bacterial and mold growth for a period of at least 12 hours; passing the initial solution through a screen and first filter to become the first filtrate, wherein the screen is gauze and the first filter has a pore size of 12 microns or less; passing the first filtrate through a vacuum filter having a pore size of 2 microns then passing the resultant filtrate through a vacuum filter having a pore size of 1.5 microns to become the second filtrate and; passing the second filtrate through a vacuum filter having a pore size of 0.22 micron, then passing the resultant filtrate through a filter having a pore size of 0.1 micron into a sterile product container.