Patent ID: 7235357
Filing Date: 2007-06-26
Classification: C12Q,G01N

Abstract:
1. A method for reducing background in fluorescence detection, which comprises: treating a solid support, to which a probe molecule to be detected is fixed, with a quenching agent so as to bind the quenching agent to the solid support; and bringing a sample containing a sample molecule labeled with a fluorescent substance into contact with the probe molecule-fixed solid support; wherein background fluorescence caused by non-specific binding of the sample molecule is quenched by the action of the quenching agent, and wherein the solid support to which the probe molecule to be detected is fixed is manufactured by: reacting a group of a charged reactive group X provided on the surface of a solid support with a compound having (a) a reactive group E at or near one end portion that can react with said reactive group X to form covalent bonding and (b) a reactive group G at or near another end portion that is the same as or different from said reactive group E, wherein said reaction between reactive groups E and X form a reactive solid support; bringing said reactive solid support that is provided with a group of reactive linking groups into contact with a group of probe molecules having a reactive group Q at or near one end wherein said reactive group Q reacts with said reactive group G to form covalent bonding; and binding the probe molecule to the linking group via covalent bonding generated by the reaction between said reactive group Q and said reactive group G.