Patent ID: 9110077
Filing Date: 2015-08-18
Classification: C07K,C12Q,G01N

Abstract:
1. A method for identifying a non-peptide small organic molecule having a molecular mass of less than 2500 Da which selectively modulates a functional cascade in a cell involving a target protein, comprising at least the following steps: a) identifying an intracellular antibody or an intracellular antibody fragment comprising at least one of the variable domains of an immunoglobulin chain, which binds to said target protein and modulates said functional cascade involving said target protein by inhibiting a protein-protein interaction, b) high-throughput screening, utilizing a library of small organic molecules, non-peptide small organic molecules having a molecular mass of less than 2500 Da that modulate the binding between said target protein and the antibody or the antibody fragment identified in a), and c) identifying, from the modulatory small organic molecules obtained in b), those which selectively modulate said functional cascade.