Patent ID: 9102952
Filing Date: 2015-08-11
Classification: C12N,C12P,C12Y

Abstract:
1. A method for the conversion of mevalonate to isoprenol, isoprene or isoamyl alcohol in vitro, comprising: (a) producing isoprenol in vitro by the step consisting essentially of incubating mevalonate with diphosphomevalonate decarboxylase and NTP, rNTP, dNTP or pyrophosphate, thereby producing isoprenol, and (b) isolating the isoprenol or further converting the isoprenol to isoprene or isoamyl alcohol, wherein the diphosphomevalonate decarboxylase comprises an amino acid sequence selected from the group consisting of SEQ ID NOs: 6, 7, 14 and 16.