Patent ID: 7638300
Filing Date: 2009-12-29
Classification: A61P,C07K,C12N,C12P

Abstract:
1. An isolated host cell, comprising: an unnatural amino acid having the structure of Formula I, wherein R is any substituent other than a substituent of any natural amino acid and wherein the unnatural amino acid is a tyrosine analogue or a phenylalanine analogue; a tyrosyl orthogonal tRNA (O-tRNA); an orthogonal aminoacyl tRNA synthetase (O—RS), wherein the ORS is derived from a tyrosyl ORS and wherein the O—RS preferentially aminoacylates the O-tRNA with the unnatural amino acid; wherein the Km of the ORS for the unnatural amino acid is lower than for any naturally occurring amino acid; wherein the kcat of the ORS for aminoacylation of the O-tRNA with the unnatural amino acid is higher than aminoacylation of the O-tRNA with any naturally occurring amino acid; wherein an mRNA that comprises a selector codon is specifically recognized by the O-tRNA; and wherein the mRNA encodes a human serum albumin (HSA) polypeptide, which mRNA comprises at least one selector codon that is recognized by the O-tRNA.