Patent ID: 7470517
Filing Date: 2008-12-30
Classification: C12Q

Abstract:
1. A method of determining a target sequence of a template nucleic acid comprising the steps of: a) creating a transcript of an isolated template nucleic acid using polymerase enzyme and nucleosides selected for sequence specific reactivity and molecular weight and oligonucleotide primers; b) performing a cleavage reaction resulting in complete cleavage of the transcript in a sequence-specific manner into fragments using cutters selected from the group consisting of enzymatic cutters, chemical cutters, and a combination thereof; c) analyzing the cleavage reaction products to determine the molecular weights of the fragments; d) performing fragment identity mapping using nucleotide masses and cleavage specificities of the cutters to calculate the molecular weights and sequences of all possible fragments that result from step b) cleavage reactions; and e) comparing the masses observed in step c) with the fragment identity mapping of step d), wherein the comparison results in determination of all the target sequences present in the sample, wherein steps a-e are performed at least two times with different cutters, thereby allowing production of overlapping fragments, and compiling the overlapping fragments to produce at least one larger subsequence.