Patent ID: 7198905
Filing Date: 2007-04-03
Classification: G01N

Abstract:
1. A method for detecting a subject in a prediabetic state comprising: measuring the D-mannose concentration in a body fluid collected from a subject at risk of or suspected of developing diabetes and comparing the D-mannose concentration with a predetermined standard value of D-mannose concentration in normal subjects; and measuring the glucose concentration in a body fluid collected from said subject, and comparing the glucose concentration with an FBS (fasting blood sugar) standard; wherein an elevated concentration of D-mannose compared to the predetermined standard value of D-mannose and a glucose concentration within a normal range concentration is indicative of a prediabetic state; wherein measuring the D-mannose concentration comprises: contacting said sample with an enzyme having oxidizing activity on mannose in the presence of an electron acceptor, and measuring the amount of reductant formed as an indication of mannose concentration.