Patent ID: 6569663
Filing Date: 2003-05-27
Classification: A61K,A61Q,C11D,C12N

Abstract:
A serine protease variant having a modified amino acid sequence of a wild-type amino acid sequence comprising a substitution by a substituting amino acid in at least one position corresponding to positions 75-76 and 78-81 of subtilisin BPNâ€² wherein:(a) when the substitution occurs at position 75, the substituting amino acid is selected from the group consisting of Ala, Arg, Asn, Cys, Gly, Phe, His, Ile, Lys, Met, Gln, Ser, Thr, Trp, Tyr, and Val; (b) when the substitution occurs at position 76, the substituting amino acid is selected from the group consisting of Ala, Arg, Cys, Ile, Leu, Met, Gln Ser, Thr, Trp, Tyr, and Val; (c) when the substitution occurs at position 78, the substituting amino acid is selected from the group consisting of Ala, Arg, Asn, Cys, Gly, Phe, His, Ile, Lys, Leu, Met, Pro, Gln, Thr, Trp, Tyr, and Val; (d) when the substitution occurs at position 79, the substituting amino acid is selected from the group consisting of Ala, Arg, Asn, Cys, Gly, Phe, His, Lys, Leu, Met, Pro, Gln, Ser, Thr, Trp, Tyr, and Val; (e) when the substitution occurs at position 80, the substituting amino acid is selected from the group consisting of Ala, Arg, Asn, Cys, Phe, His, Ile, Lys, Leu, Met, Pro, Gln, Ser, Thr, Trp, Tyr, and Val; and, (f) when the substitution occurs at position 81, the substituting amino acid is selected from the group consisting of Ala, Arg, Asn, Cys, Gly, Phe, His, Ile, Lys, Leu, Met, Pro, Gln Ser, Thr, Trp, and Tyr.