Patent ID: 8119371
Filing Date: 2012-02-21
Classification: C12P

Abstract:
1. A method of cultivation of a production strain of Bacillus polymyxa comprising, cultivating the strain in a fermentation broth at a temperature of 28° C. in a fermenter with agitation and aeration of the fermentation broth, while maintaining the pH during cultivation in a range of 5.3 to 5.6, adding a solution of glucose during cultivation to maintain a glucose concentration in a range of 0.5 to 2.5 g/l adding ammonium ions during cultivation to maintain an ammonium ion concentration in a range of 0.3 to 0.6 g/l, and filtering the fermentation broth after 44 to 56 hours of cultivation to provide a concentration of polymyxin B in the filtrate of 1.8 to 2.8 g/l as measured by HPLC method.