Patent ID: 8232049
Filing Date: 2012-07-31
Classification: C12Q

Abstract:
1. A method for detecting at least one small oligonucleotide in a biological isolate, comprising: providing a biological isolate that includes at least one small oligonucleotide; adding at least one detection oligonucleotide having a label moiety and at least one bridge oligonucleotide, the bridge oligonucleotide complementary to the detection oligonucleotide and the at least one small oligonucleotide, to the biological isolate under conditions such that the at least one small oligonucleotide and the at least one detection oligonucleotide are preferentially captured by the bridge oligonucleotide to produce at least one labeled small oligonucleotide; adding at least one ligating reagent to preferentially join the at least one captured small oligonucleotide and the at least one captured detection oligonucleotide bound to the bridge oligonucleotide; adding at least one clean-up reagent, the at least one clean-up reagent removing the label moiety from at least one detection oligonucleotide that is not ligated to at least one small oligonucleotide; detecting the at least one labeled small oligonucleotide; and determining the amount of small oligonucleotide in the biological isolate.