Patent ID: 8481270
Filing Date: 2013-07-09
Classification: C12Q,G01N

Abstract:
1. A method for chromogenic detection of two or more target molecules in a single tissue sample, comprising in sequential order: contacting the tissue sample with a first specific binding moiety that specifically binds a first target molecule; detecting the first target molecule in the tissue sample by depositing an insoluble, electron-rich aromatic chromogen product; treating the tissue sample with a solution comprising a soluble, electron-rich aromatic compound; contacting the tissue sample with a second, hapten-labeled specific binding moiety that specifically binds a second target molecule, where a hapten of the second, hapten-labeled specific binding moiety comprises an electron-deficient aromatic compound; and, detecting the second target molecule by depositing a second, insoluble chromogen product that is distinguishable from the insoluble, electron-rich aromatic compound deposited to detect the first target molecule, where treating the tissue sample with the solution containing the soluble, electron-rich aromatic compound reduces background due to non-specific binding of the hapten-labeled specific binding moiety to the insoluble electron rich compound deposited near the first target molecule.