Patent ID: 7425430
Filing Date: 2008-09-16
Classification: C12Q,G01N

Abstract:
1. A probe for imaging protein phosphorylation and dephosphorylation, which comprises: (a) a peptide substrate domain containing a phosphorylation site having an amino acid residue selected from tyrosine, serine and threonine; (b) a phosphorylation recognition domain that is a single chain antibody obtained using the phosphorylated peptide substrate domain as an antigen, or an endogenous domain selected from SH2 domain, phosphotyrosine binding domain and WW domain, and specifically interacts and recognizes phosphorylation of the peptide substrate domain; and (c) a peptide linker sequence, wherein: (i) the peptide substrate domain and the phosphorylation recognition domain are bound together by the peptide linker sequence and interposed between a donor chromophore and an acceptor chromophore that cause fluorescence resonance energy transfer (FRET) to occur; and (ii) the peptide substrate domain, the linker sequence and the phosphorylation recognition domain are linked in order from N-terminal to C-terminal of the probe.