Patent ID: 8753812
Filing Date: 2014-06-17
Classification: B82Y,C12Q,G01N

Abstract:
1. A method for nucleic acid sequence detection, comprising: (a) providing a reaction medium including at least one template nucleic acid molecule with components for template polymerization by complimentary addition of one or more nucleotides to the at least one template nucleic acid molecule with a concomitant proton release; (b) contacting the reaction medium with a polarizable electrode responsive to charge perturbations in the reaction medium; (c) inducing changes in polarity of the polarizable electrode in response to surface charge perturbations in the polarizable electrode resulting from the concomitant proton release into the reaction medium contacting the polarizable electrode as template polymerization of the at least one template nucleic acid molecule occurs; (d) generating current signals in the polarizable electrode in response to the changes in polarity induced in step (c) in the polarizable electrode, and amplifying the current signals with an amplifying circuit having an input connected to the polarizable electrode; and (e) associating amplified current signals generated in step (d) with one or more corresponding nucleotides added to the at least one template nucleic acid to thereby discern at least a portion of the at least one template nucleic acid sequence.