Patent ID: 8865470
Filing Date: 2014-10-21
Classification: G01N,Y10S,Y10T

Abstract:
1. A method for determining, in an instrument for analyzing a multiplicity of fluorescent dyes using a multiplicity of detector channels, a compensation value for the photodetector gain settings from an uncalibrated spillover value of a fluorescent dye that emits in a primary detection channel and a secondary detection channel, wherein the method comprises: a) performing a first set of measurements, in the primary and secondary detection channels of the fluorescent dye, of emissions of the fluorescent dye and a fluorescent reference material that emits in the primary and secondary detection channels of the fluorescent dye to obtain a first set of reference values associated with a first set of signal amplification settings on the instrument; b) adjusting the instrument to a second set of signal amplification settings; c) performing a second set of measurements, in the primary and secondary detection channels of the fluorescent dye, of emissions of the fluorescent dye and the reference material to obtain a second set of reference values associated with the second set of signal amplification settings; d) calculating a calibrated spillover value from the first set of reference values and determining the uncalibrated spillover value from the calibrated spillover value and the second set of reference values, wherein the uncalibrated spillover value is dependent on the second set of signal amplification settings and the calibrated spillover value is independent of the second set of signal amplification settings; and e) determining the compensation value from the uncalibrated spillover value of the fluorescent dye.