Patent ID: 8030037
Filing Date: 2011-10-04
Classification: C12P,Y10S

Abstract:
1. A method of preparing a concentrated microalgae composition comprising: cultivating microalgae photoautotrophically outdoors in open ponds under filtered sunlight in continuous or batch mode at a dilution rate of less than 35% per day; harvesting the microalgae in exponential phase when cell number is increasing at a rate of at least 20% of maximal rate of growth; and concentrating the microalgae to obtain a concentrated microalgae composition having at least 40% of lipids in the microalgae in the form of glycodiacylglycerides, phosphodiacylglycerides, or a combination thereof and at least 5% by weight of fatty acids are docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), or a combination thereof; wherein (a) the microalgae are