Patent ID: 7960144
Filing Date: 2011-06-14
Classification: C07K,C12N

Abstract:
1. A method for assessing the folding state of a test polypeptide, comprising: (a) providing an expression vector comprising a polynucleotide encoding a fluorescent protein circular permutant derived from superfolder GFP, set forth as SEQ ID NO:4, in which a polynucleotide encoding the test polypeptide is inserted in-frame within said polypeptide; (b) culturing a cell containing the expression vector of (a) under conditions sufficient to permit expression of the fluorescent protein circular permutant-test polypeptide fusion protein; and (c) detecting fluorescence in the cell and thereby assessing the folding state of the test polypeptide, wherein folding of the test polypeptide correlates with the degree of fluorescence exhibited by the expressed fusion protein.