Patent ID: 6270977
Filing Date: 2001-08-07
Classification: C12Q

Abstract:
A kit for detecting the presence or absence of pseudorabies virus in a sample using a nested polymerase chain reaction, said kit comprising at least two nucleotide sequences for an outer pair of oligonucleotide primers used in a first-stage polymerase chain reaction, and at least two nucleotide sequences for an inner pair of oligonucleotide primers used in a second-stage polymerase chain reaction, wherein said outer pair and inner pair of primers each consist of a 5' upper primer and a 3' lower primer, wherein the nucleotide sequence comprising the upper primer of the outer primer pair is SEQ ID NO: 2, the nucleotide sequence comprising the lower primer of the outer primer pair is selected from the group consisting of SEQ ID NOS: 3 to 4, the nucleotide sequence comprising the upper primer of the inner primer pair is selected from the group consisting of SEQ ID NOS: 5 to 7, and the nucleotide sequence comprising the lower primer of the inner primer pair is selected from the group consisting of SEQ ID NOS: 8 to 11.