Patent ID: 6194212
Filing Date: 2001-02-27
Classification: A61K,A61P,C07K,C12N

Abstract:
A method of using a DNA scaffold attachment region (SAR) to increase gene expression in retrovirally transduced eukaryotic resting cells comprising,(a) transducing eukaryotic cells with a retroviral vector including (i) a heterologous gene operatively linked to an expression control sequence and (ii) a SAR wherein said SAR corresponds to a SAR of the human interferon .beta. gene, a fragment thereof having at least 450 base pairs, or a sequence having 90% homology thereto; and(b) allowing said transduced eukaryotic cells to achieve resting state and express the heterologous gene wherein said expression in the transduced eukaryotic resting cells is increased compared to transduction with said vector including said heterologous gene and lacking the SAR.