Patent ID: 6251278
Filing Date: 2001-06-26
Classification: B01D,B01J,G01N

Abstract:
A process for the separation of at least one substance from a mixture comprising the steps of:(a) contacting a mixture containing the at least one substance with an affinity ligand retained on a composition to bind said substance to said affinity ligand, said composition having the formula S-B-X-Y-L wherein S is a substantially non-compressible solid support comprising silica, B is a binding group containing silicon covalently linked to a silanol group of S through a siloxane bond, X is a substantially non-ionic hydrophilic spacer comprising polyethylene glycol having between 4 and 300 ethylene glycol units, Y is a coupling group and L is said affinity ligand, and(b) passing an eluting solution through the chromatographic material to recover the at least one substance from said composition, wherein the adsorption rate constant for said binding is greater than that for the same process using a composition having the formula S-B-L, andwherein said process is performed at a pressure greater than 500 psi and a linear flow velocity greater than approximately 0.1 cm/min.