Patent ID: 8753819
Filing Date: 2014-06-17
Classification: C12Q

Abstract:
1. A nucleic acid assay for detecting echinocandin drug resistance mutations in fungi susceptible to echinocandin drugs and containing the FKS1 gene corresponding to 1-3β-D-glucan synthase subunit Fks1p comprising exponentially amplifying a first nucleic acid target sequence of the FKS1 gene corresponding to at least a portion of CaFks1p amino acids 636 to 654 that includes amino acids 641 to 649 to generate an amplified first target sequence, contacting the amplified first target sequence with a first labeled hybridization probe specific for an amino acid mutation selected from the group consisting of S645P, S645Y, and S645F of CaFks1p, and detecting the presence of the mutation, thereby detecting an echinocandin drug resistance mutation.