Patent ID: 8445224
Filing Date: 2013-05-21
Classification: A61P,C12Q,G01N

Abstract:
1. A method for assaying FTO activity, the method comprising contacting a FTO polypeptide, with oxygen and 2-oxoglutarate as co-substrates and optionally a methylated nucleic acid substrate, and iron as a cofactor, and measuring oxygenase activity of said FTO polypeptide compared to a control, wherein said FTO polypeptide comprises: (a) the amino acid sequence of SEQ ID NO: 1, (b) an amino acid sequence having at least 80% identity to the amino acid sequence of SEQ ID NO: 1 over its full length, or (c) an amino acid sequence comprising amino acids 10 to 495 of SEQ ID NO: 1.