Patent ID: 8293488
Filing Date: 2012-10-23
Classification: G01N

Abstract:
1. A method of identifying a neurogenic agent, which comprises: (a) providing a stable human neural progenitor cell line, wherein (b) plating undifferentiated cells of said cell line into a culture with a serum-free, mitogen-free medium; (c) contacting the cells with a test agent which is a fused imidazole, an aminopyrimidine, a nicotinamide, an aminomethyl phenoxypiperidine or an aryloxypiperidine; (d) incubating the cells in the presence of the test agent for 3-7 days; (e) quantitatively determining the formation of neurons from said cells; and identifying the test agent as a neurogenic agent if said test agent increases the number or proportion of neurons by at least ten percent (10%).