Patent ID: 6221625
Filing Date: 2001-04-24
Classification: G01N,Y10S

Abstract:
An enzyme-labeled immunoassay comprising the steps of:providing an absorbent material capable of transporting a developing solution by capillary action,applying a test sample containing an analyte to the absorbent material,allowing the analyte to react with an enzyme-labeled reagent, the reaction being allowed in the absorbent material,immobilizing the reacted enzyme-labeled reagent,allowing a substrate to react with the immobilized enzyme to form a signal, wherein, by the formation of the signal or no formation of the signal, a presence or absence of the analyte is detected,preventing a further signal formation from a predetermined time on after the immobilization of the enzyme-labeled reagent, using an enzyme inhibitor transported in the absorbent material, andevaluating the formation of the signal, whereby the presence of the analyte contained in the test sample is detected.