Patent ID: 6586229
Filing Date: 2003-07-01
Classification: C12N,C12P

Abstract:
An isolated nucleic acid fragment encoding genes involved in a bacterial toluene monooxygenase enzyme pathway selected from the group consisting of:(a) an isolated nucleic acid fragment encoding the amino acid sequence selected from the group consisting of SEQ ID NOs:2 and 92; (b) an isolated nucleic acid fragment that is chemically equivalent to an isolated nucleic acid fragment encoding the amino acid sequence selected from the group consisting of SEQ ID NOs:2 and 92; (c) an isolated nucleic acid fragment encoding a polypeptide of at least 111 amino acids having at least 60% identity based on the Smith-Waterman method of alignment with the amino acid sequence selected from the group consisting of SEQ ID NOs:2 and 92; (d) an isolated nucleic acid fragment that hybridizes with (a) under hybridization conditions of 0.1Ã—SSC, 0.1% SDS, 65Â° C. and washed with 2Ã—SSC, 0.1% SDS followed by 0.1Ã—SSC, 0.1% SDS; and (e) an isolated nucleic acid fragment that is complementary to (a), (b), (c), or (d).