Patent ID: 8697363
Filing Date: 2014-04-15
Classification: C12N,C12Q,Y10T

Abstract:
1. An assay method for detecting a plurality of target nucleic acids in a plurality of samples, the method comprising: providing S samples that will be mixed together prior to assay, where S is an integer greater than 1; separately subjecting each of said S samples to an encoding reaction that produces a set of T tagged target nucleotide sequences, each tagged target nucleotide sequence comprising a sample-specific nucleotide tag and a target nucleotide sequence; wherein T is the number of target nucleic acids to be detected, T being an integer greater than one; mixing together tagged target nucleotide sequences from said S samples to form an assay mixture; dividing the assay mixture into S×T amplification mixtures, wherein S×T is at least 30, and separately and simultaneously subjecting each of said amplification mixtures to amplification using a unique pair of amplification primers, wherein each amplification primer pair comprises: for each unique primer pair, determining whether an amplification product is present in the amplification mixture; and if the amplification product is present, determining that a particular target nucleic acid is present in a particular sample; or if the amplification product is not present, determining that the particular target nucleic acid is not present in the particular sample.