Patent ID: 8580531
Filing Date: 2013-11-12
Classification: G01N,Y10T

Abstract:
1. An in vitro method for identifying a plurality of antibodies in a biological sample against antigenic molecules carried by erythrocytes, said antigenic molecules comprising phosphatidylserine, human leukocyte antigen (HLA) antigens, chemical products or medicaments or degradation products thereof, by bringing said biological sample into contact, in a single test receptacle, or in several separate test receptacles, with groups of distinguishable beads, each group of distinguishable beads carrying (1) erythrocytes carrying said antigenic molecules or (2) erythrocyte membrane fragments carrying said antigenic molecules the antigenic molecules carried by (1) erythrocytes or (2) erythrocyte membrane fragments differing between each group of distinguishable beads, under conditions which allow the antibodies or activated serum complement fractions present in the sample to bind to said antigenic molecules on the erythrocytes or the erythrocyte membrane fragments without agglutination, eliminating the antibodies or activated serum complement fractions which have not bound to antigenic molecules carried by said erythrocytes or said erythrocyte membrane fragments, labelling antibodies bound to said antigenic molecules and/or the bound activated serum complement fractions, and analyzing the mixture so as to identify the group of beads having bound the labelled antibodies or the labelled activated serum complement fractions, thereby allowing the identification of the antibodies present in said biological sample that specifically bind to said antigenic molecules carried by said erythrocytes or said erythrocyte membrane fragments.