Patent ID: 7029839
Filing Date: 2006-04-18
Classification: A01N

Abstract:
1. A method of preserving at freezer temperatures mammalian donor organs for transplantation comprising: cooling a loading, pre-freezer preservation solution at least containing polyvinylpyrrolidone -40, a calcium channel blocker, a nucleoside, potassium chloride, polyethylene glycol, at least one amino acid and a steroid to a temperature between 2° C. and 4° C.; cooling a quantity of the loading, prefreezer preservation solution to a temperature between 0° C. and 2° C.; cooling a cryopreservation solution at least containing polyvinylpyrrolidone -40, a calcium channel blocker, a nucleoside, potassium chloride, polyethylene glycol, at least one amino acid, a steroid, glycerol, propanediol, an alcohol and an alkyl sulfoxide to a temperature between 0° C. and 2° C.; inserting a needle into the isolated arterial system of the donor organ before removal from a donor; infusing the donor organ with the loading, pre-freezer preservation solution cooled to a temperature between 2° C. and 4° C. through the needle; harvesting the donor organ; immersing for 10 minutes or longer the donor organ in the loading, pre-freezer preservation solution cooled to a temperature between 0° to 2° C.; infusing the donor organ with the cryopreservation solution cooled to a temperature between 0° to 2° C. through the needle in a gradual manner; immersing for 10 minutes or longer the donor organ in the cryopreservation solution cooled to a temperature of 0° C. to 2° C.; storing the donor organ in the cryopreservation solution at a temperature below 0° C.