Patent ID: 6280941
Filing Date: 2001-08-28
Classification: C12Q,Y10S

Abstract:
A method of detecting a genetic predisposition in a human subject to develop systemic lupus erythematosus (SLE), comprising:a) collecting a tissue sample from a human subject;b) amplifying nucleic acids from said tissue sample using a forward primer having nucleotide sequence GAT TCC CCA TCT CTC TTT CTT T (SID. NO. 1) and a reverse primer having nucleotide sequence AAA TTG TGG TAA TGA CTG CA (SEQ ID. NO. 2) to obtain amplification products, said nucleic acids comprising a genomic sequence of human chromosome lq41-q42 between microsatellite markers D1S2860 and D1S213; andc) detecting in the amplification products the presence or absence of a fragment of 85-base pair length, the presence of said fragment of 85-base pair length indicating a genetic predisposition in said subject to develop SLE.