Patent ID: 6140097
Filing Date: 2000-10-31
Classification: C12N,C12Y

Abstract:
A mesophilic xylanase I enzyme, derived from an Acremonium celluloyticus mold, having the following properties:(a) an action: the enzyme hydrolyzes xylan non-specifically mainly into xylose, xylobiose and xylotriose,(b) a substrate specificity: the enzyme acts on xylan,(c) optimal pH and stable pH: the enzyme has an optimal pH at 3.5 and is stable at a pH of 3.5-9.5, at 25.degree. C. for 2 hours as determined by saccharifying activity using soluble xylan as a substrate,(d) an optimal temperature for action at 55.degree. C. as determined by saccharifying activity using soluble xylan as a substrate,(e) a temperature stability: the enzyme is stable at 55.degree. C. or lower at a pH 3.5 for 10 minutes,(f) molecular weights of 30,000 as determined by SDS-polyacrylamide gel electrophoresis, and 69,500 when determined by gel filtration chromatography, and(g) specific activities of 112.1 U/mg-protein as determined by saccharifying activity for soluble xylan as a substrate, and 76.6 U/mg-protein as determined by saccharifying activity for insoluble xylan as a substrate.