Patent ID: 6103479
Filing Date: 2000-08-15
Classification: B01J,C12Q,G01N

Abstract:
A method for producing a cassette for cell screening, comprising:a) providing a base with a surfaceb) preparing a micro-patterned chemical array;c) modifying the micro-patterned chemical array to produce a modified micro-patterned chemical array comprising multiple different cell binding sites on the surface of the base, wherein the different cell binding sites interact with different cell types, wherein a cell binding site comprises a well;d) binding cells to the modified micro-patterned chemical array to produce an ordered array of cell types seeded on the wells; ande) providing a fluid delivery system for delivering a combinatorial of reagents to the ordered array of cell types; wherein said fluid delivery system comprises a chamber that mates with the base containing the ordered array of cell types, wherein the chamber comprises:(i) etched domains matching the wells on the surface of the base, and(ii) microfluidic channels that supply fluid to the etched domains.