Patent ID: 6183994
Filing Date: 2001-02-06
Classification: C12P

Abstract:
A method of producing an amino-deoxy di- or oligosaccharide compound which either consists of or is a fragment or analog of a carbohydrate part in a glycoconjugate, said method comprising(1) reacting(a) at least one donor substance comprising a mono, di- or oligosaccharide, a glycoside or a derivative thereof,(b) at least one acceptor substance comprising an amino-deoxy mono-, di-, or oligosaccharide, or glycoside thereof, and(c) an E.C. group 3.2 glycosidase or a modified 3.2 glycosidase to form an amino-deoxy di- or oligosaccharide compound,wherein said glycosidase is a recombinant glycosidase, and(2) optionally isolating said amino-deoxy di- or oligosaccharide compound.