Patent ID: 6368600
Filing Date: 2002-04-09
Classification: A61K,A61P,C07K

Abstract:
An isolated Dirofilaria immitis nucleic acid molecule that encodes a cuticlin protein or that is complementary to a nucleic acid molecule which encodes a cuticlin protein, wherein said Dirofilaria immitis nucleic acid molecule hybridizes to a nucleic acid sequence selected from the group consisting of SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, and SEQ ID NO:10 under conditions comprising (a) hybridizing in a solution comprising 17.53 grams of sodium chloride and 8.82 grams sodium citrate in 0.1 liters of water, pH 7 (2Ã—SSC) in the absence of nucleic acid helix destabilizing agents, at a temperature of 37Â° C., and (b) washing in a solution comprising 8.765 grams of sodium chloride and 4.41 grams sodium citrate in 0.05 liters of water, pH 7 (1Ã—SSC) in the absence of nucleic acid helix destabilizing agents at a temperature of 64Â° C.