Patent ID: 6013457
Filing Date: 2000-01-11
Classification: C12N,G01N

Abstract:
A method for immunometrically detecting and/or determining the concentration of an analyte, comprising the steps of:1) contacting a sample containing the analyte with:a) a labeled tracer, which binds to the analyte to form a labeled complex;b) a receptor A, which binds to the labeled complex;c) a receptor B, which binds to the labeled tracer and generates or alters, a signal by interacting with the labeled tracer,wherein receptors A and B are immobilized at a distance sufficient to prevent all or most of the labeled complexes from simultaneously binding to both an A and a B receptor;2) determining a signal generated by the labeled tracer;3) analyzing the signal to detect and/or determine the concentration of analyte in the sample.