Patent ID: 8685655
Filing Date: 2014-04-01
Classification: C07K

Abstract:
1. A method for dissociating avidin or streptavidin from a complex of (a) avidin or streptavidin and (b) a first biotin derivative, comprising: combining (a) avidin or streptavidin immobilized on an insoluble carrier and (b) a first biotin derivative to form a complex, wherein the first biotin derivative has a cyclic moiety different from a cyclic moiety of biotin and binds to avidin or streptavidin with a lower affinity than biotin, having an affinity binding constant to avidin of 10 dissociating avidin or streptavidin from the a first biotin derivative with a disassociation agent in a liquid sample under a physiological condition, wherein the disassociation agent comprises biotin covalently bound to a water-soluble polymer or a second biotin derivative covalently bound to the water-soluble polymer, wherein the water-soluble polymer has a density of 5 to 1,000 molecules of the biotin or the second biotin derivative per molecule of the water-soluble polymer, wherein the second biotin derivative has the same cyclic moiety as biotin; and wherein the dissociation agent can dissociate avidin or streptavidin from the first biotin derivative with a dissociation efficiency of at least 70% and more efficiently than the biotin or the second biotin derivative not bound to the polymer.