Patent ID: 6986887
Filing Date: 2006-01-17
Classification: A01K,A61K,C07K,C12N

Abstract:
1. An in vitro method of altering the histocompatibility phenotype of a human stem cell, comprising: a) deleting adjacent HLA-B and HLA-C MHC genes from a first chromosome; b) replacing HLA-B and HLA-C MHC genes on a second chromosome with a site specific cassette, said cassette comprised within a vector that includes a site specific recombination sequence selected from LoxP and FRT; c) inserting a transgene harboring a heterologous HLA allele and a homologous sequence matching the site specific recombination sequence of step b); and d) catalyzing said site specific recombination wherein said transgene becomes incorporated into said second chromosome by site specific recombination to provide a stem cell with altered histocompatibility phenotype.