Patent ID: 6660495
Filing Date: 2003-12-09
Classification: A61K,A61P,C07K

Abstract:
A preparation for inducing tolerance in an individual, said preparation comprisingdepigmented and oxidized proteinaceous components from allergenic source material, and wherein the proteinaceous components have a molecular weight in the range of 1-10 kDa, said preparation being obtainable according to the method of (a) dissolving proteinaceous components from an allergenic source material aqueous solution; (b) separation of the dissolved proteinaceous components from the allergenic source material; (c) removal of material with a molecular weight lower than 3.5 kDa from the dissolved proteinaceous components to provide a HMW-N fraction; (d) depigmentation of the HMW-N fraction to provide a HMW-D fraction; (e) oxidation of the HMW-D fraction by an oxidizing agent, ultraviolet irradiation or a combination of both, to provide a HMW-Dox fraction; (f) digestion of the HMW-Dox fraction with a proteolytic enzyme until the majority of the proteinaceous components in the HMW-Dox have a molecular weight between 1 and 10 kDa, and (g) recovery of the proteinaceous components with a molecular weight between 1 and 10 kDa and formulation into a preparation for inducing tolerance in an individual.