Patent ID: 8999140
Filing Date: 2015-04-07
Classification: C12N,C12Q,C12Y,G01N

Abstract:
1. A glucose oxidase mutant modified at one or more amino acid positions selected from: (a). a position corresponding to position 53 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Thr with another amino acid residue; (b). a position corresponding to position 116 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Ile with another amino acid residue; (c). a position corresponding to position 132 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Ser or Thr with another amino acid residue; (d). a position corresponding to position 134 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Thr with another amino acid residue; (e). a position corresponding to position 237 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Ile or Phe with another amino acid residue; (f). a position corresponding to position 371 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Val or Ala with another amino acid residue; (g). a position corresponding to position 373 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Phe with another amino acid residue; (h). a position corresponding to position 434 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Glu with another amino acid residue; (i). a position corresponding to position 436 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Phe with another amino acid residue; (j). a position corresponding to position 448 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Trp with another amino acid residue; and (k). a position corresponding to position 537 of the amino acid sequence set forth in SEQ ID NO: 1 by substituting the amino acid residue Trp with another amino acid residue.