Patent ID: 8119346
Filing Date: 2012-02-21
Classification: C12Q,Y10S

Abstract:
1. A method for producing a stabilized nucleic acid duplex, the method comprising: (a) providing (i) a sample containing or suspected of containing a target nucleic acid molecule; (ii) an oligonucleotide complementary or partially complementary to said target nucleic acid molecule; and (iii) at least one soluble thiazine dye present at a concentration effective to stabilize a duplex formed between said target nucleic acid molecule and said oligonucleotide, wherein said target nucleic acid, said oligonucleotide and said thiazine dye are all in solution in free form; and (b) either (i) annealing said target nucleic acid and said oligonucleotide in the presence of said thiazine dye; or (ii) annealing said target nucleic acid and said oligonucleotide, followed by admixing with said thiazine dye; under conditions wherein base-pairing occurs to produce at least one stabilized nucleic acid duplex.