Patent ID: 9080142
Filing Date: 2015-07-14
Classification: A01H,A61P,C07K,C12N,G01N

Abstract:
1. A method for identifying a plant that is resistant to i) a plant virus named Tomato Marchitez virus (ToMarV), deposited under depositors reference PRI-TMarV0601 on Jul. 10, 2007 with the Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH, in Braunschweig, Germany, the deposited material receiving the accession number DSM 19656, or ii) a Tomato Marchitez virus comprising at least one nucleic acid sequence selected from the group consisting of SEQ ID NO: 16 and a sequence having a nucleotide sequence homology of at least 80% thereto, said virus having isometric virus particles with a diameter of approximately 28 nm, having a viral genome consisting of two (+) ssRNA molecules of about 7.2 kb (RNA1) and about 4.9 kb (RNA2), wherein the viral capsid contains three coat proteins of approximately 35, 26 and 24 kDa, respectively, and wherein the virus induces severe leaf necrosis in tomato plants, beginning at the base of the leaflets, and necrotic rings on tomato fruits, the method comprising the steps of: