Patent ID: 7662596
Filing Date: 2010-02-16
Classification: C12N,C12P

Abstract:
1. A method for producing (4R)-4-(indole-3-ylmethyl)-4-hydroxy-2-oxoglutaric acid (4R-IHOG) of formula (1) or a salt thereof: comprising: reacting indole-3-pyruvic acid with pyruvic acid or oxaloacetic acid in the presence of a protein or a microorganism containing said protein to produce 4R-IHOG having an optical purity of at least 70%, wherein said protein is selected from the group consisting of: (a) a protein comprising the amino acid sequence of SEQ ID NO: 15, and (b) a protein that is at least 95% sequence identical to the protein of SEQ ID NO: 15 and has 4R-aldolase activity.