Patent ID: 6194178
Filing Date: 2001-02-27
Classification: C12N,C12P,C12Q

Abstract:
A method for the enzymatic synthesis of an .alpha.-2,3 sialylated fucosylated oligosaccharide containing a sialic acid or analogue thereof which method comprises the steps of:a) selecting an .alpha.-2,3 sialyltransferase compatible with a fucosylated oligosaccharide having a fucose group in the non-reducing penultimate saccharide position;b) selecting a CMP-sialic acid or an analogue thereof which is compatible with the sialyltransferase selected in step (a);c) contacting said CMP-sialic acid or an analogue thereof with a fucosylated oligosaccharide of the formula ##STR4##wherein R.sub.1 represents a saccharide residue, R.sub.2 represents a saccharide residue, and R.sub.1 and R.sub.2 together represent an acceptor for the selected sialyltransferase; n is from 0 to about 10, Y is selected from the group consisting of O, NH and S, and R.sub.3 is selected from the group consisting of a protein, a lipid or an aglycon moiety having at least one carbon atom, in the presence of the sialyltransferase selected in (a) above under conditions whereby the sialic acid or analogue thereof is transferred from the CMP-sialic acid or analogue thereof to the non-reducing sugar terminus of the fucosylated oligosaccharide so as to form an .alpha.-2,3 sialylated fucosylated oligosaccharide containing a sialic acid or analogue thereof.