Patent ID: 7642048
Filing Date: 2010-01-05
Classification: C12Q,G01N

Abstract:
1. A method for testing sensitivity of a tumor to a chemotherapeutic agent or combination, comprising: mincing an epithelial tumor specimen to prepare a plurality of multicellular tissue explants, and placing the explants in liquid medium in one or more containers; physically agitating the explants, culturing said explants to form one or more non-confluent monolayers, and suspending the non-confluent monolayer(s) in culture medium to obtain from about 4,000 to about 12,000 cells per ml; transferring the cells to a plurality of wells at about 100 to about 1000 cells per well; adding a serial dilution of at least one chemotherapeutic agent or combination across the wells; and determining sensitivity of the cells to the chemotherapeutic agent or combination.