Patent ID: 6645758
Filing Date: 2003-11-11
Classification: B01L,G01N

Abstract:
A closed, disposable cuvette for carrying out amplification and detection of nucleic acid material, comprising:a plurality of compartments defined by walls, including a reaction compartment, said reaction compartment containing nucleic acid material and amplifying reagents, at least one of said walls of at least one of said compartments being constructed to allow transfer of heat between the one compartment and the external environment; at least one detection material being present in at least one of said compartments; and means for fluidly interconnecting said compartments in prescribed order when pressure is applied to the contents of a compartment; said compartments all being closed to fluid flow to locations outside of the cuvette; at least one of said compartments including means at a detection site therein for immobilizing at said site said nucleic acid material for detection after amplification; whereby detection of amplified nucleic acid material occurs without contamination by the amplified nucleic acid material of other cuvettes for the assay of the same nucleic acid material.