Patent ID: 6187573
Filing Date: 2001-02-13
Classification: C12N,C12Q

Abstract:
A method for purifying DNA polymerase obtainable from a KOD1 strain which belongs to a Hyperthermophilic archaeon, comprising culturing recombinant host cells transfopled by a recombinant DNA expression vector that comprises a foreign DNA sequence inserted into a vector, wherein the foreign DNA sequence encodes the thermostable DNA polymerase derived from a KOD1 strain which bclongs to Hyperthermophilic archaeon, having an amino acid sequence of SEQ ID No. 1 and/or a nucleotide sequence of SEQ ID No. 5 and further (a) recovering the cultured recombinant host cells, lysing them and preparing the cell extract, and (b) removing the impurified proteins derived from recombinant host cells.