Patent ID: 7695952
Filing Date: 2010-04-13
Classification: B01F,B01L,C12N

Abstract:
1. A disposable sample processing module for processing DNA or RNA samples, such module comprising: a hybridization chamber adapted to receive an oligonucleotide covalently bonded to an internal surface of the hybridization chamber; a sample well adapted to hold a DNA or RNA sample, said sample well being coupled to the hybridization chamber; a moveable valve plate disposed between the sample well and hybridization chamber, where a bottom of the sample well is in direct contact with a top of the valve plate, the valve plate having a preexisting channel extending through the moveable valve plate, said moveable valve plate having a first position relative to the sample well and hybridization chamber where the channel of the valve plate completes a passageway between the hybridization chamber and sample well and that allows transfer of the DNA or RNA sample from the sample well to the hybridization chamber and a second position relative to the sample well and hybridization chamber where a body of the valve plate blocks the passageway to prevent transfer to the hybridization chamber; and a manifold adapted to exchange fluids with the hybridization chamber to hybridize the DNA or RNA sample with the oligonucleotide, to wash the hybridized sample and to amplify the hybridized sample.