Patent ID: 7914995
Filing Date: 2011-03-29
Classification: C12Q

Abstract:
1. A reaction mixture for pyrophosphorolysis activated polymerization (PAP) amplification of a region of a template nucleic acid comprising (i) a template nucleic acid, (ii) a first oligonucleotide primer having a non-extendible 3′ terminus, (iii) a second oligonucleotide primer, (iv) a DNA polymerase, (v) pyrophosphate and (vi) deoxyribonucleotides, wherein the first oligonucleotide primer with the non-extendible 3′ terminus is incapable of being extended by the DNA polymerase, wherein the non-extendible 3′ terminus is removable by pyrophosphorolysis, wherein one of the first and second oligonucleotide primers is capable of hybridizing to a sense strand of the template nucleic acid and the other is capable of hybridizing to an antisense strand of the template nucleic acid, and wherein the first and second oligonucleotide primers flank the region of the template nucleic acid to be amplified.