Patent ID: 6624621
Filing Date: 2003-09-23
Classification: G01N

Abstract:
An apparatus for counting and sensing the volume of particles by the Coulter method comprising vacuum means to move particles suspended in an electrolyte through an orifice of predetermined diameter having rounded entry and exit corners in an electrically insulating material and with an electrode in contact with said electrolyte before said orifice and a second electrode in contact with said electrolyte after said orifice used to pass a constant electric current through said orifice and having an amplifier to increase the voltage pulse produced across said electrodes by the passage of said particles through said orifice wherein the improvement comprises the following:(a) electrically resistive material disposed before, within, and after said orifice wherein said orifice entry and exit corners are rounded with a radius of up to one half of said orifice axial length whereby the electric field strength across the minimum diameter of said orifice is substantially uniform thereby permitting use of a diameter of a conduit approximately equal to the minimum diameter of said orifice; (b) a conduit of predetermined diameter passing through said electrically resistive material approximately concentric with said orifice wherein the corners of said conduit inlet and outlet are approximately 90 degrees and have a corner/radius less than 10% of the radius of said conduit; (c) said electrically resistive material having an electrical resistivity approximately equal to the electrical resistivity of said electrolyte, whereby all said particles passing through said conduit are guided through a region of substantially identical maximum electric field strength thereby producing substantially identical maximum said voltage pulses for substantially identical said particles and whereby said particles which recirculate downstream of said conduit produce effectively negligible said voltage pulses.