Patent ID: 8080647
Filing Date: 2011-12-20
Classification: C12N

Abstract:
1. An isolated polynucleotide encoding a tetracycline repressor (TetR) polypeptide that binds to a tetracycline operator (TetOp) in the absence of tetracycline or tetracyclone analog, wherein the polynucleotide comprises a nucleic acid molecule modified by codon substitution of SEQ ID NO: 1, wherein codons having a higher frequency of usage in a plant are substituted for codons having a lower frequency of usage in a plant, wherein % GC content of the nucleic acid molecule has been increased to at least 50%, and wherein the isolated polynucleotide encodes a TetR polypeptide having at least 95% sequence identity to SEQ ID NO: 2, wherein the % sequence identity is based on the entire coding region and is calculated by the GAP algorithm under default parameters, the nucleic acid molecule having at least 97% sequence identity to SEQ ID NO: 3, 5, 11 or 13.