Patent ID: 7186889
Filing Date: 2007-03-06
Classification: C12N

Abstract:
1. A method for genetic transformation of Eucalyptus spp. , which comprises the following steps: sterilization and washing of seeds of transference of said seeds to an appropriate medium of culture; germination for a period between about 2 and 17 days; collection of germinated material; inoculation with inoculation between about 20 and 30 hours in a liquid medium; transference of the material to a solid medium for a period between about 38 and 50 hours in the dark, under temperature between about 25 and 31° C. and ambient humidity; transference of the material to start plantlet growth in the light; withdrawal of a plantlet leaf obtained between about 5 and 17 days during growth in the light; transference of the plantlet leaf to the a MS medium containing phenyl urea; germination of the plant tissue for approximately 20 days; and identification and selection of the transformed plantlets.