Patent ID: 7285391
Filing Date: 2007-10-23
Classification: C07K,G01N,Y10S

Abstract:
1. A method for detecting non-trophoblast malignancy in a sample comprising: (a) contacting the sample with a first antibody which specifically binds to the early pregnancy associated molecular isoform of hCG under conditions permitting formation of a complex between the antibody and the early pregnancy associated molecular isoform of hCG, wherein the first antibody is B152 produced by the hybridoma deposited with the American Type Culture Collection under Designation No. HB-12467 and the complex is detected by B207 antibody produced by the hybridoma deposited with the American Type Culture Collection under ATCC Designation No. PTA-1626; (b) contacting the sample with a second antibody which specifically binds to intact non-nicked hCG without cross-reacting with the first antibody under conditions permitting formation of a complex between the second antibody and the early pregnancy associated molecular isoform of hCG; (c) measuring the amount of complexes formed in steps (a) and (b; and (d) comparing the amount of complex formed in step (a) with the amount of complex formed in step (b), wherein a positive detection of complex formed in step (a) and a relative absence of complex formed in step (b) indicates the presence of non-trophoblast malignancy in the sample.