Patent ID: 6521423
Filing Date: 2003-02-18
Classification: A61K,A61P,C07K,C12N,Y10S

Abstract:
A method for isolating a hepatitis C virus (HCV) E2 polypeptide that lacks a portion of its C-terminus from about amino acid 715 to its C-terminus, numbered with reference to the HCV1 E2 amino acid sequence, said method comprising:(a) providing a population of host mammalian, yeast, plant or bacterial cells transformed with a polynucleotide comprising a coding sequence for said HCV E2 polypeptide, wherein said coding sequence is operably linked to control elements such that said coding sequence can be transcribed and translated in the host cell; (b) culturing said population of host cells under conditions whereby said HCV E2 polypeptide is expressed intracellularly; (c) disrupting said host cells; and (d) isolating said HCV E2 polypeptide from said disrupted host cells.