Patent ID: 8883495
Filing Date: 2014-11-11
Classification: A61K,A61P,C12N

Abstract:
1. A human T-cell population obtainable by coculturing a mononuclear cell collected from human blood with a stroma cell, wherein: (a) the human blood is selected from the group consisting of cord blood and peripheral blood; (b) the human T-cell population contains cells that express CD3; (c) the human T-cell population contains cells that express CD25; (d) the human T-cell population contains cells that express CD28; (e) the human T-cell population contains cells that express T-cell antigen receptor αβ; (f) the human T-cell population contains subpopulations (i), (ii), and (iii), wherein subpopulation (i) is a CD4 positive and CD8 positive (CD4 subpopulation (ii) is a CD4 positive and CD8 dimly positive (CD4 subpopulation (iii) is a CD4 negative and CD8 positive (CD4 (g) wherein at least 90% of the total cells in the human T-cell population are composed of subpopulation (i), subpopulation (ii), and subpopulation (iii); (h) the human T-cell population is free of human or mouse stroma cells; and (i) the human T-cell population produces interleukin-10 (IL-10).