Patent ID: 6054288
Filing Date: 2000-04-25
Classification: A61K,C07K,C12N

Abstract:
A method of providing a therapeutic product in an effective amount to a mammal, comprising the steps of:a) obtaining a source of primary cells from a mammal;b) transfecting primary cells obtained in (a) with a DNA construct comprising exogenous DNA encoding the therapeutic product and additional DNA sequences sufficient for expression of the exogenous DNA in the primary cells, thereby producing transfected primary cells which express the exogenous DNA encoding the therapeutic product;c) culturing a transfected primary cell produced in (b), which expresses the exogenous DNA encoding the therapeutic product, under conditions appropriate for propagating the transfected primary cell which expresses the exogenous DNA encoding the therapeutic product, thereby producing a clonal cell strain of transfected secondary cells from the transfected primary cell;d) culturing the clonal cell strain of transfected secondary cells produced in (c) under conditions appropriate for and sufficient time for the clonal cell strain of transfected secondary cells to undergo a sufficient number of doublings to provide a sufficient number of transfected secondary cells to produce an effective amount of the therapeutic product; ande) introducing transfected secondary cells produced in (d) into a mammal in sufficient number to provide an effective amount of the therapeutic product to the mammal.