Patent ID: 8945850
Filing Date: 2015-02-03
Classification: C12Q

Abstract:
1. A method for the detection and/or identification of Staphylococcus aureus in a sample, comprising the steps of (i) hybridizing the polynucleic acids of the sample with at least two polynucleotide probes that hybridize to a target sequence within 25 nucleotides of each other, wherein the at least two polynucleotide probes are SEQ ID NO:17 and SEQ ID NO:19, and wherein the target sequence comprises at least 50 consecutive nucleotides from a nucleic acid molecule of SEQ ID NO:1 or SEQ ID NO: 2 or a a the RNA form of SEQ ID NO: 1 wherein T is replaced by U, or the RNA form of SEQ ID NO 2 wherein T is replaced by U, or the RNA form of said the RNA form of said the sequence complementary to SEQ ID NO:1 the sequence complementary to SEQ ID NO:2 the sequence complementary to the the sequence complementary to the the sequence complementary to the RNA form of said SEQ ID NO:1 wherein T is replaced by U, or the sequence complementary to the RNA form of said SEQ ID NO:2 wherein T is replaced by U, or the sequence complementary to the RNA form of said the sequence complementary to the RNA form of said (ii) detecting the hybrids formed as a result of (i), and (iii) detecting or identifying wherein the method comprises the step of amplifying the 16S-23S rRNA spacer region, or a fragment comprising the target sequence, or the target sequence in a PCR amplification preceding step (i), wherein the PCR amplification comprises the use of the primers of SEQ ID NO:68 and SEQ ID NO:58.