Patent ID: 7855051
Filing Date: 2010-12-21
Classification: C12Q

Abstract:
1. A method for detecting the presence of mycoplasma in a sample comprising: a) contacting a sample treated to release ribosomal RNA of mycoplasma present in the sample with two or more first oligonucleotide probes wherein each first oligonucleotide probe is substantially complementary to a portion of a 16S ribosomal RNA of at least one mycoplasma species and is labeled with a capture ligand, the first oligonucleotide probes comprising SEQ ID NO's 14 and 15; b) contacting the sample with two or more second oligonucleotide probes wherein each second oligonucleotide probe is substantially complementary to a different portion of the 16S ribosomal RNA of the at least one mycoplasma species and is labeled with a first component of a detection system, the first component comprising a detection ligand, the second oligonucleotide probes comprising SEQ ID NO's 5 and 10; c) incubating the sample with the first and second oligonucleotide probes under hybridization conditions to form a hybridization solution and for a time sufficient for the probes to hybridize to 16S ribosomal RNA of mycoplasma species present in the sample; d) contacting the hybridization solution with a solid phase coated with a capture receptor capable of specifically binding to the capture ligand of the first labeled oligonucleotide probe(s); and e) detecting the presence of mycoplasma ribosomal RNA in the sample with a detection solution comprising a labeled detection receptor.