Patent ID: 7604960
Filing Date: 2009-10-20
Classification: C07K,C12N

Abstract:
1. A method for producing a protein of interest, the method comprising: a) providing a recombinant adenoviral vector comprising nucleic acid encoding the protein of interest under control of a promoter, wherein said recombinant adenoviral vector has deletions in the E1 and the E2A regions of the adenovirus genome, b) propagating said recombinant adenoviral vector in complementing cells that express adenoviral E1 protein and adenoviral E2A protein, to obtain recombinant adenovirus particles, c) infecting a culture of complementing cells that express adenoviral E1 protein, but not adenoviral E2A protein, with said recombinant adenovirus particles, to produce the protein of interest, and d) harvesting the protein of interest.