Patent ID: 8560246
Filing Date: 2013-10-15
Classification: C12Q

Abstract:
1. An automated method for simultaneously managing results from a real-time polymerase chain reaction (PCR) instrument for the identification of samples comprising high-titer nucleic acids, the method comprising: (a) obtaining fluorescence data from sequence detection software (SDS) associated with a real-time PCR instrument; (b) independently calculating, from the data obtained from the SDS associated with the real-time PCR instrument, a normalized fluorescence signal (R (c) determining whether the normalized fluorescence signal (R (d) flagging the sample as a potentially high-titer sample when the normalized fluorescence signal (R (e) comparing the SDS-calculated fluorescence data obtained from the SDS associated with the real-time PCR instrument to the independently calculated normalized fluorescence signal (R (f) verifying that the SDS-calculated fluorescence data are substantially accurate.