Patent ID: 6821781
Filing Date: 2004-11-23
Classification: C12N

Abstract:
A method of selecting plant cells that contain a non-native nucleic acid of interest, comprising transforming plant cells with a first chimeric nucleic acid containing in operable association a first promoter functional in a plant cell and a first DNA molecule of interest, and a second chimeric nucleic acid containing in operable association a second promoter and a second DNA molecule encoding a cystathionine gamma synthase that permits the selection of transformed plant cells transformed with said chimeric nucleic acid molecules by rendering said transformed plant cells resistant to an amount of ethionine that would be toxic to plant cells that do not express said second DNA molecule; culturing the plant cells in medium containing ethionine in an amount that would be toxic to plant cells that do not express said second DNA molecule; and selecting plant cells that grow in said medium.