Patent ID: 6916612
Filing Date: 2005-07-12
Classification: C07H,C40B,G01N,Y10S

Abstract:
1. A set of electrophoretic probes for detecting the presence or absence of one or more of a plurality nucleotide sequences in a sample, the set comprising a plurality of electrophoretic probes selected from the group defined by the formula: wherein: (D, M)-N is an e-tag reporter released from an electrophoretic probe of the set upon digestion of the electrophoretic probe by a nuclease, the e-tag reporter having a charge; D is a detection moiety; M is a mobility modifier consisting of from 1 to 500 atoms selected from the group consisting of carbon, hydrogen, oxygen, phosphorus, nitrogen, sulfur, and boron; N is a nucleotide; and T is an oligonucleotide specific for at least one of the plurality of nucleotide sequences, each T having a length in the range of from 12 to 60 nucleotides such that at least one nucleotide of T has a capture ligand attached; and wherein each e-tag reporter of the plurality of electrophoretic probes has a distinct charge/mass ratio so that e-tag reporters of different electrophoretic probes form distinct peaks in an electropherogram upon electrophoretic separation; and wherein the capture ligand specifically binds to a capture agent that has a charge opposite to that of e-tag reporters so that undigested electrophoretic probes are excluded from the electropherogram.