Patent ID: 6350595
Filing Date: 2002-02-26
Classification: C07H,Y10T

Abstract:
A process for the chemical synthesis of a polynucleotide having a totally or partially random sequence, said random sequence being constituted by trinucleotide sequence units coding for amino acids according to the degeneracy of the genetic code, wherein said polynucleotide is synthesized by:a) utilizing a pre-synthesized mononucleotide and a pre-synthesized dinucleotide as a monomeric synthesis unit, said mononucleotide and dinucleotide being a sequence unit of said trinucleotide sequence units; b) carrying out the synthesis of said polynucleotide on at least one support, wherein, on said support, first reaction cycles, in which a mixture of said dinucleotides is bound, are alternated with second reaction cycles in which one mononucleotide is bound, and wherein at least one alternation between the first and second reaction cycle is predefined in order to form trinucleotide sequence units with a reduced number of mononucleotide and dinucleotide sequence units relative to the mononucleotide and dinucleotide sequence units forming said trinucleotide sequence units according to the genetic code, thereby reducing the degeneracy effects of the genetic code; and c) repeating step b) until said polynucleotide is of desired length.