Patent ID: 6537542
Filing Date: 2003-03-25
Classification: A61K

Abstract:
A method of producing a therapeutic product in a mammal, said method comprising the steps of:a) providing a DNA construct comprising: 1) exogenous DNA encoding a product to be expressed in a primary or secondary cell of mammalian origin; 2) DNA sequences homologous with genomic DNA sequences in a primary or secondary cell of mammalian origin; and 3) DNA sequences encoding a selectable marker; b) introducing into a primary or secondary mammalian cell the DNA construct provided in (a), thereby producing a primary or secondary cell comprising the DNA construct provided in (a); c) maintaining a primary or secondary cell produced in (b) so that homologous recombination occurs between DNA sequences of said DNA construct that are homologous with genomic DNA sequences of the cell and the genomic DNA sequences of the cell, thereby producing a homologously recombinant primary or secondary cell of mammalian origin having the DNA construct of (a) integrated into genomic DNA of the primary or secondary cell; and d) introducing a sufficient number of homologously recombinant primary or secondary cell produced in (c) into a mammal to produce the therapeutic product in the mammal.