Patent ID: 7320860
Filing Date: 2008-01-22
Classification: C12Q

Abstract:
1. A method of analyzing a circularized nucleic acid, comprising amplifying the circularized nucleic acid to provide an amplification product which comprises a concatemer of a sequence to be analyzed, the method further comprising: (i) monomerizing said amplification product using a restriction enzyme and an oligonucleotide complementary to the amplification product which is present in excess over the number of monomers contained in the amplification product, wherein the restriction enzyme cleaves any amplification product-oligonucleotide hybrids; (ii) circularizing said monomers derived from the amplification product by hybridization to uncleaved oligonucleotide remaining from step (i) and ligation; (iii) amplifying said circularized monomers in a rolling-circle amplification reaction; (iv) optionally repeating steps (i) to (iii); and (v) detecting or analyzing the amplification products.