Patent ID: 8637257
Filing Date: 2014-01-28
Classification: A61K,A61P,C12N,C12Y,G01N

Abstract:
1. A method for identifying a compound that inhibits bone resorption, comprising: (a) contacting a compound with a polypeptide comprising the amino acid sequence of SEQ ID NO: 59 or a nucleic acid encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 59; and (b) measuring a compound polypeptide property, wherein said property is the expression or enzymatic activity of said polypeptide; and (c) contacting a population of isolated mammalian cells in culture expressing said polypeptide with one or more compound(s) from step (b) that significantly inhibits the expression or enzymatic activity of the polypeptide; and (d) identifying one or more compound(s) that increases osteoprotegerin (OPG) levels in said culture; wherein a compound that inhibits bone resorption is identified.