Patent ID: 7056687
Filing Date: 2006-06-06
Classification: C07K,C12N,C12Q,G01N

Abstract:
1. A method of screening for cells having an altered phenotype, the method comprising the steps of: a) providing a population of cells having a parent phenotype, said population of cells comprising a nucleic acid sequence encoding a first element expressed in said population of cells; b) introducing into said population of cells a library of fusion nucleic acids, said fusion nucleic acids each comprising: c) modulating the expression of said nucleic acid sequence encoding the candidate bioactive agent by contacting said population of cells with a third element, wherein said modulation is selected from the group consisting of induction and repression of said nucleic acid sequence encoding the candidate bioactive agent; d) collecting a first subpopulation of cells having an altered phenotype; e) modulating in the opposite direction of step (c) the expression of said nucleic acid sequence by modulating said contacting of first subpopulation of cells with said third element; f) collecting a second subpopulation of cells having said parent phenotype; g) modulating in the same direction as in step (c) the expression of said nucleic acid sequence by contacting said second subpopulation of cells with said third element; and h) detecting a third subpopulation of cells having said altered phenotype.