Patent ID: 7575882
Filing Date: 2009-08-18
Classification: A61K,A61P,G01N

Abstract:
1. A method of identifying a compound that decreases the biological activity of TRPV2, comprising the steps of: a. contacting a TRPV2 polypeptide with a cannabinoid that is capable of activating the TRPV2 activity under a condition in which the TRPV2 is activated by the cannabinoid, wherein said TRPV2 polypeptide comprises a sequence selected from the group consisting of SEQ ID NO:2, a sequence having greater than about 70% amino acid sequence identity to SEQ ID NO:2, a functional fragment of SEQ ID NO:2 wherein said functional fragment of SEQ ID NO: 2 maintaining the biological activity of TRPV2 and comprising amino acids 66 to 729 of SEQ ID NO:2, a sequence having greater than about 70% amino acid sequence identity to said functional fragment of SEQ ID NO:2, a deletion or modification of SEQ ID NO:2 that maintains said functional fragment of TRPV2, and a sequence having greater than 70% amino acid sequence identity to a deletion or modification of SEQ ID NO:2 that maintains said functional fragment of TRPV2; b. contacting the TRPV2 polypeptide with a test compound; c. measuring the biological activity of the TRPV2 in the presence of both the cannabinoid and the test compound; d. repeating step a); e. measuring the biological activity of the TRPV2 in the presence of the cannabinoid but not the test compound; and f. comparing the TRPV2 activity measured from step c) with that from step e); thereby identifying the compound that decreases the biological activity of TRPV2 when the TRPV2 activity measured from step c) is less than that from step e).