Patent ID: 6040196
Filing Date: 2000-03-21
Classification: G01N,Y10S

Abstract:
A method of evaluating an amount of antigen or antibody immobilized on a solid phase carrier comprising a calcium phosphate compound, of a test element, the method comprising:(a) contacting the test element with a known amount of a first antibody or antigen which specifically binds to the immobilized antigen or antibody, respectively, to form a first immunocomplex;(b) contacting the first immunocomplex with an enzyme-labelled antigen or antibody which specifically binds to the first antibody or antigen to form a labelled immunocomplex;(c) contacting the labelled immunocomplex with a substrate which reacts with the enzyme of the enzyme-labelled antigen or antibody to produce a color; and(d) comparing the amount of the color produced on the test element to a standard color produced by a reference test element with a known amount of the immobilized antigen or antibody thereon processed according to (a)-(c) to determine the amount of the antigen or antibody immobilized on the solid phase carrier of the test element.