Patent ID: 7828948
Filing Date: 2010-11-09
Classification: B01D,G01N

Abstract:
1. A microfluidic device comprising a microfluidic channel having at least one in situ polymerized size-exclusion polymeric element having a first side and a second side, and at least one porous polymeric element adjacent to the second side, wherein the size-exclusion polymeric element has a length that is thinner than the length of the porous polymeric element, a loading channel in fluidic communication with a portion of the microfluidic channel containing the porous polymeric element, a sample reservoir in fluidic communication with a first waste reservoir, said sample reservoir is configured for containing a sample, wherein the sample reservoir and the first waste reservoir are configured to apply a first electric field from the sample reservoir through the loading channel and from the second side to the first side to the first waste reservoir, and a first buffer reservoir and a second waste reservoir configured to apply a second electric field from the first buffer reservoir and from the second side to the second waste reservoir through the length of the porous polymeric element, wherein the second electric field bypasses the size-exclusion polymeric element.