Patent ID: 6365376
Filing Date: 2002-04-02
Classification: C12Q

Abstract:
An isolated nucleic acid fragment encoding an adipic acid synthesizing protein selected from the group consisting of:(a) an isolated nucleic acid molecule encoding the amino acid sequence set forth in SEQ ID NO:2, encoding a transcription factor; SEQ ID NO:4, encoding a caprolactone hydrolase; SEQ ID NO:6, encoding a cyclohexanone monooxygenase; SEQ ID NO:8, encoding a 6-hydroxy hexanoic acid dehydrogenase; SEQ ID NO:12, encoding a cyclohexanol dehydrogenase; SEQ ID NO:14, encoding a cyclohexanol dehydrogenase; SEQ ID NO:18, encoding a 6-hydroxy hexanoic acid dehydrogenase; SEQ ID NO:20, encoding a regulatory protein; SEQ ID NO:22, encoding a cyclohexanone monooxygenase; or SEQ ID NO:24, encoding a transcription factor; and (b) an isolated nucleic acid molecule that hybridizes with an isolated nucleic acid molecule encoding the amino acid sequence set forth in SEQ ID NO:2, encoding a transcription factor, SEQ ID NO:4, encoding a caprolactone hydrolase; SEQ ID NO:6, encoding a cyclohexanone monooxygenase; SEQ ID NO:8, encoding a 6-hydroxy hexanoic acid dehydrogenase; SEQ ID NO:12, encoding a cyclohexanol dehydrogenase; SEQ ID NO:14, encoding a cyclohexanol dehydrogenase; SEQ ID NO:18, encoding a 6-hydroxy hexanoic acid dehydrogenase; SEQ ID NO:20, encoding a transcription factor; SEQ ID NO:22, encoding a cyclohexanone monooxygenase; or SEQ ID NO:24, encoding a transcription factor; under the following hybridization conditions: 0.1Ã—SSC, 0.1% SDS at 65Â° C.; and washed with 2Ã—SSC, 0.1% SDS followed by 0.1Ã—SSC, 0.1% SDS; or an isolated nucleic acid molecule that is completely complementary to (a) or (b).