Patent ID: 8999683
Filing Date: 2015-04-07
Classification: C12N,C12P,H04W

Abstract:
1. A genetically modified microorganism for the extracellular production of free fatty acids and esters thereof, wherein said microorganism characterised by: a. reduced fatty acyl-coA synthetase activity conferred by a FAA2 gene deletion; b. enhanced acetyl CoA carboxylase expression conferred by a recombinant endogenous ACC1 gene wherein said gene is operably linked to a heterologous promoter; and c. a transgene encoding a pyruvate formate lyase comprising PflA and PflB, wherein said microorganism is selected from among species of Aspergillus nidulans, Candida tropicalis, Saccharomyces cerevisiae, Schizosacchromyces pombe, and Yarrowia lipolytica.