Patent ID: 6180363
Filing Date: 2001-01-30
Classification: C12N

Abstract:
A method of producing a protein in yeast comprising:(a) selecting a protein encoded by a reference DNA sequence that comprises at least one region of ten consecutive codons comprising at least two codons that have a first frequency of use by yeast of less than or equal to 13 per 1000 codons;(b) replacing at least 50% of codons that have a first frequency of use codons in the reference DNA sequence with having a second frequency of use by yeast of greater than or equal to 15 per 1000 codons and encoding the same amino acid as the codon being replaced to provide a modified sequence;(c) transforming yeast with a vector comprising said modified sequence under the control of yeast regulatory elements; and(d) culturing said transformed yeast under conditions whereby said modified sequence is expressed to provide said protein.