Patent ID: 6632631
Filing Date: 2003-10-14
Classification: C12N,C12Q,C12Y

Abstract:
A method for identifying a test compound as a candidate for an antibiotic, comprising:a) contacting a homocitrate synthase polypeptide with a test compound, wherein the homocitrate synthase polypeptide is selected from the group consisting of Spongospora subterrane, Botrytis cinerea, Armillaria mellea, Ganoderma adspersum, Piptoporus betulinus, Ustilago maydis, Polyporus squamosus, Cercospora zeae-maydis, Armillaria gallica, Armillaria luteobubalina, Armillaria ostoyae, Colletotrichum musae, Monilinia fructigena, Penicillium expansum, Plasmodiophora brassicae, Phytophthora infestans, Heterobasidion annosum, Gaeumannomyces graminis, Ophiostoma ulmi, Uromyces appendiculatus, Cochliobolus carbonum, Periconia circinata, Cochliobolus heterostrophus, Cochliobolus lunata, Cochliobolus stenospilus, Fusarium oxysporum, Fusarium graminearum, Fusarium culmorum, Rhizoctonia solani, Puccinia graminis, Sclerotinia sclerotiorum, Magnaporthe rhizophila, Magnaporthe salvinii, Magnaporthe grisea, Magnaporthe poae and Magnaporthe Pyricularia; and b) detecting the presence or absence of binding between said test compound and said homocitrate synthase polypeptide; wherein binding indicates that said test compound is a candidate for an antibiotic.