Patent ID: 6368875
Filing Date: 2002-04-09
Classification: G01N

Abstract:
A method for the detection of at least one analyte, which comprises:a) applying a sample solution suspected of containing an analyte to a particle zone of a device that can detect such an analyte, the device comprising a solid support containing (1) a particle zone that contains particles containing a first member of an inverse ligand-receptor pair, a first member of a direct ligand-receptor pair, and a signal reagent; and (2) a first and a second signal zone wherein the first signal zone contains a second member of the inverse ligand-receptor pair and the second signal zone contains a second member of the direct ligand-receptor pair wherein: the analyte is a member of the inverse ligand-receptor pair or an analog thereof and is not a member of the direct ligand-receptor pair or analog thereof; b) flowing the solution sequentially through the particle zone and then the signal zones; and c) determining the ratio of signals between the first and second signal zones.