Patent ID: 7439032
Filing Date: 2008-10-21
Classification: C12Q,G01N

Abstract:
1. A method of screening for an agent useful for sensitizing a cancer cell to DNA damage by a second agent, which comprises: (i) contacting a human β-transducin repeat containing protein (β-TrCP) β-TrCP1 or β-TrCP2 with a test compound and a phosphorylated cell division cycle 25A (Cdc25A) protein or a phosphorylated fragment thereof of at least 8 amino acids in length; (ii) detecting a reduced binding of the β-TrCP1 or β-TrCP2 to the phosphorylated Cdc25A protein or fragment thereof in the presence of the test compound as compared to a control; and (iii) selecting the test compound that reduces the binding as an agent useful for sensitizing a cancer cell to DNA damage by the second agent; wherein the control is the binding of the β-TrCP1 or β-TrCP2 to the Cdc25A protein or fragment thereof in the absence of the test compound.