Patent ID: 8450084
Filing Date: 2013-05-28
Classification: C12P

Abstract:
1. A method for preparing a peptide comprising coupling an activated, N-protected, amino acid C-terminal ester or an activated, optionally N-protected, peptide C-terminal ester, the coupling comprising activating a t-alkyl C-terminal ester of a first N-protected amino acid or a t-alkyl C-terminal ester of a first, optionally N-protected, peptide by transesterification with an activating alcohol, the activation being catalysed by a peptidase (E.C. 3.4), thereby forming a second N-protected amino acid C-terminal ester or a second, optionally N-protected, peptide C-terminal ester; and wherein the transesterification takes place in a liquid phase comprising the activating alcohol, which liquid phase comprises less than 2 wt. % water, based on the total weight of the liquid phase; and coupling the second ester with an, optionally C-terminal protected, amino acid or an, optionally C-terminal protected, peptide forming a peptidic bond, the coupling being catalysed by an enzyme catalysing peptidic bond formation, which enzyme may be the same as or different from the enzyme used for catalysing the transesterification.