Patent ID: 8835160
Filing Date: 2014-09-16
Classification: C12N

Abstract:
1. A method for creating a synthetic genetic circuit comprising: a) providing: b) applying said second vector to said host cell such that recombination occurs between said antibiotic resistance sequence in said first vector and said first and second homologous sequences thereby causing insertion of said different antibiotic resistance sequence and said genetic elements of interest at a location within said antibiotic resistance sequence of said host cell's genome which destroys the function of said antibiotic resistance sequence, and c) applying said third vector to said host cell such that recombination occurs between said different antibiotic resistance sequence in said second vector and said third and fourth homologous sequences thereby causing insertion of said additional antibiotic resistance sequence and said genetic elements of interest at a location within said different antibiotic resistance sequence of said host cell's genome which destroys the function of said different antibiotic resistance sequence, wherein said genetic elements of interest comprise first and second terminators, thereby generating a synthetic genetic circuit.