Patent ID: 7270947
Filing Date: 2007-09-18
Classification: C12P

Abstract:
1. A method for controlling a biooxidation reaction for preparing carboxylic acids comprising the steps of: (1) independently adding a substrate and a co-substrate at predetermined rates to a biooxidation reaction mixture comprised of a biocatalyst; (2) measuring the oxygen consumption rate and carbon dioxide evolution rate from the reaction mixture; (3) determining the instantaneous rates of substrate (−r  the co-substrate combustion stoichiometry of the form,  and a constitutive stoichiometric model for the yield of biomass formation having anabolic reactions of the form,  wherein, the simultaneous equations are given by (4) simultaneously adjusting the substrate and co-substrate addition rates, either continuously or periodically during the biooxidation reaction, relative to the rates of substrate oxidation and co-substrate consumption according to the control formulas respectively, using dimensionally consistent units of measure, thereby maximizing the rate of product formation while minimizing the rate of co-substrate usage, using adjustable parameters A