Patent ID: 6521428
Filing Date: 2003-02-18
Classification: C12Q,Y10T

Abstract:
A method of sequencing a of a nucleic acid template comprising:(a) providing a plurality of first primers, each first primer comprising (i) a region of different fixed nucleotide sequence having a defined length of from about 5 to 15 bases long and (ii) a region of randomized nucleotide sequence having a defined length of from about 2 to 11 bases long located 5â€² to or 3â€² to the region of fixed nucleotide sequence; (b) annealing the plurality of first primers to different locations on a nucleic acid template, wherein at least one primer from within the plurality of first primers anneals specifically to the template; (c) extending the specifically annealed primer from step (b) with a mixture of dNTPs and ddNTPs to generate a series of nucleic acid fragments; and (d) determining the nucleotide sequence of a first region of the template from the series of nucleic acid fragments.