Patent ID: 6194562
Filing Date: 2001-02-27
Classification: C12N

Abstract:
A method of reducing the concentration of endotoxins in a solution containing a target nucleic acid and endotoxins, the method comprising:a. providing a first silica matrix consisting essentially of silica, wherein the first silica matrix is equilibrated with a first equilibration buffer, wherein the equilibration is substantially free of chaotropic agents;b. providing a first solution comprising a target nucleic acid and endotoxins, wherein the first solution is substantially free of chaotropic agents;c. combining the first silica matrix and the first solution, wherein at least one of the endotoxins forms an endotoxin/matrix complex with the first silica matrix, thereby producing a second solution which contains fewer of the endotoxins than are present in the first solution; andd. separating the second solution from the endotoxin/matrix complex.