Patent ID: 6486384
Filing Date: 2002-11-26
Classification: A01H,C12N,Y02A

Abstract:
A method for transforming a plant comprising:(a) culturing an isolated meristematic tissue of the plant on a meristem proliferation medium so as to produce adventitious meristematic cells in the isolated meristematic tissue; (b) after step (a), introducing a nucleic acid into at least one of the adventitious meristematic cells, thereby producing a transformed cell comprising the nucleic acid; (c) culturing the transformed cell on meristem proliferation medium so as to promote growth of transformed meristematic cells; (d) selecting transformed meristematic cells or at least one structure thereof; and (e) regenerating a transformed plant from the transformed meristematic cells or structure thereof; wherein the plant is a cereal plant, the nucleic acid is introduced into the adventitious meristematic cell by particle bombardment, the meristematic tissue is a shoot apex and the meristem proliferation medium comprises from 0 mg/L to about 3 mg/L of an auxin, from about 2 mg/L to about 8 mg/L of a cytokinin, from about 10 g/L to about 60 g/L of maltose or about 10 g/L to about 60 g/L of sucrose; and from about 0.1 &mgr;M to about 50 &mgr;M copper.