Patent ID: 8273573
Filing Date: 2012-09-25
Classification: B01D,B01F,B01J,B01L,B82Y,C07K,C12N,C12Q,C12Y,C30B,G01N,Y10T

Abstract:
1. A method for obtaining a collection of plugs comprising at least one biological molecule and, optionally, at least one or more chemical reactants for conducting a reaction with the biological molecule, the method comprising the steps of: providing a microfluidic system comprising at least a first and second channel, the first and second channels configured to intersect with each other at a junction, and an outlet adapted for receiving a segment of tubing or a sample tube; continuously flowing an aqueous fluid containing the at least one biological molecule and, optionally, the at least one or more reagents for conducting the reaction with the biological molecule, through the first channel; continuously flowing a carrier fluid immiscible with the aqueous fluid through the second channel; forming a plurality of the plugs of the aqueous fluid containing the at least one biological molecule and, optionally, the at least one or more reagents for conducting the reaction with the biological molecule, by partitioning the aqueous fluid with the flowing immiscible carrier fluid at the junction of the first and second channels, the plugs being substantially surrounded by the immiscible carrier fluid flowing through the channel, wherein at least one plug of the plurality comprises the at least one biological molecule and, optionally, the at least one or more reagents for conducting the reaction with the biological molecule; and collecting the plurality of the plugs in the segment of tubing or the sample tube, wherein the plugs remain separated by the carrier immiscible fluid.