Patent ID: 8652769
Filing Date: 2014-02-18
Classification: A01N,G01N

Abstract:
1. A method of producing a frozen-thawed sorted artificial insemination sample comprising: thawing frozen sperm cells; staining said thawed sperm cells with a concentration of a Hoechst 33342 greater than 40 micromolar; establishing the staining temperature between about 30° C. and about 40 C.°; determining a sex characteristic of said sperm cells; separating said sperm cells according to the determination of their sex characteristic; isolating sperm cells separated according to the determination of their sex characteristic in a collection element at a rate of greater than 1000 sperm per second for either X-chromosome bearing sperm or Y-chromosome bearing sperm; establishing a frozen-thawed sorted artificial insemination sample from said sperm cells isolated in said collection element; and fertilizing an egg with said frozen-thawed sorted artificial insemination sample at success levels of at least about 70% of the success levels with sperm cells that have not been separated and/or frozen.