Patent ID: 6090568
Filing Date: 2000-07-18
Classification: G01N,Y10S

Abstract:
A competitive immunoassay for determining an analyte in a sample comprising hemoglobin and said analyte, comprising:a) providing a dry multilayer immunoassay element comprising in order,(i) a spreading layer comprising particles having a light absorbing material encapsulated therein,(ii) a receptor layer comprising an immobilized receptor which specifically binds to said analyte or an analog thereof, and(iii) a radiation-transmissive support;b) contacting the spreading layer with said sample;c) contacting the spreading layer with less than or about 5.times.10.sup.-10 M label reagent comprising a peroxidase label conjugated to said analyte or an analog thereof;d) contacting the spreading layer with a solution comprising a chemiluminesence reagent admixture which reacts with the peroxidase label to produce a chemiluminescent signal and which is effective to separate unbound label reagent from label reagent bound to said immobilized receptor; ande) measuring the chemiluminescent signal produced by said bound label reagent to determine said analyte in said sample.