Patent ID: 8062859
Filing Date: 2011-11-22
Classification: A61P,G01N

Abstract:
1. A method for screening anti-metastatic anticancer substances which do not act directly on the expression of E-cadherin, said method comprising the following steps: a) growing tumour cells with the capacity for forming metastases and which do not express E-cadherin on their cell membrane; b) brining the cultivated cells obtained at the end of step a) into contact with a candidate substance or a combination of candidate substances; c) detecting, in the culture of cells obtained at the end of step b), the presence of E-cadherin at the cell surface; and d) positively selecting the candidate substance, or the combination of candidate substances, when the E-cadherin has been detected at step c), wherein the tumour cells that have the capacity to from metastases are from cell lines selected from the group consisting of: SW620 (ATTC No. CCL-227), SKCo-1 (ATTC No. HTB-39) and CoLo205 (ATTC No. CCLL-222).