Patent ID: 7785899
Filing Date: 2010-08-31
Classification: G01N,Y10S

Abstract:
1. A lateral flow test strip for the determination of a concentration of an analyte in a liquid sample comprising: a) a stationary phase membrane in contact or contacted with a mobile-phase composition and having a first end and a second end, wherein the membrane allows lateral capillary flow of the sample from the first end to the second end, said mobile phase composition having a labeled receptor which is capable of binding to the analyte to form a labeled receptor-analyte complex; b) a test zone on the membrane, the test zone comprising a plurality of spaced apart test areas, each test area having immobilized thereon a test area capture agent, each of the capture agents characterized by a greater binding affinity to the labeled receptor than to the labeled receptor-analyte complex; c) a control zone having a control zone capture agent immobilized on the membrane, the control zone capture agent characterized in that the control zone capture agent has affinity to both the labeled receptor and to the labeled receptor-analyte complex, wherein the binding of the labeled receptor to the test areas and control zone provides a detectable signal with a measurable intensity that is used to determine the concentration of analyte in the sample and wherein the intensity of the detectable signals in the test areas are inversely related to the concentration of analyte in the sample.