Patent ID: 6221238
Filing Date: 2001-04-24
Classification: C12Q

Abstract:
An enzymatic-electrochemical one-step affinity sensor for the quantitative determination of analytes in aqueous media, comprising a support having applied thereon a measuring system or two adjacent measuring systems of identical design, and the contact paths thereof,wherein each measuring system comprises multiple consecutive layers arranged over a redox electrode modified with an electron mediator, and a pseudo-reference electrode,wherein said layers are laterally encapsulated in a liquid-proof fashion by a fixing frame having a top cover which has an area having openings to receive the sample to be measured,and wherein said layers comprise1) eithera) a layer including a phenol oxidase substrate, for that case where a marker enzyme of an affine binding partner is a phenol oxidase, orb) a layer including a hydrolase substrate and an additional layer in the vicinity of the electrode which includes immobilized phenol oxidase, for that case where a marker enzyme of an affine binding partner is a hydrolase,2) a sample-receiving and reservoir layer,3) a layer in the vicinity of the electrode, and4)a) layers which contain affine binding partners, orb) a layer including appropriate immobilized affinity complexes.