Patent ID: 6451987
Filing Date: 2002-09-17
Classification: C07K,C12N

Abstract:
A method for purifying a peptide from a mixture comprising said peptide and related impurities, said method comprising:a) eluting said related impurities of said mixture from a cation exchange chromatography matrix using a solution comprising an organic modifier, water, and a buffer, and optionally a salt component, at a linear or step gradient or isocratically in the salt component, and at pH-values optionally maintained with a buffer so that said peptide has a positive local or overall net charge and said related impurities have a local or overall positive net charge which is lower than the positive net charge of said peptide so as to remove said related impurities; and b) subsequently, eluting said peptide in the absence of the organic modifier, by a step or linear change to an aqueous solvent optionally with a salt component, at the same or higher pH-values optionally maintained with a buffer.