Patent ID: 6949629
Filing Date: 2005-09-27
Classification: C07K

Abstract:
1. A method for purifying a protein wherein after purification said protein is of at least 90% purity, is substantially free of cross-reacting antigens and antibodies, and is selected from the group consisting of: proteins having at least about 86% amino acid identity with CEA; proteins that immunologically cross-react with CEA; and proteins encoded by a gene selected from the group consisting of CEACAM1, CEACAM2, CEACAM3, CEACAM4, CEACAM5, CEACAM6, CEACAM7, CEACAM8, AG128375, PSG1, PSG2, PSG3, PSG4, PSG5, PSG6, PSG7, PSG8, PSG9, PSG10, PSG11, CEACAM-ps1, CEACAM-ps2, CEACAM-ps3, CEACAM-ps4, CEACAM-ps5, CEACAM-ps6, CEACAM-ps7, CEACAM-ps8, CEACAM-ps9, CEACAM-ps10, and CEACAM-ps11; said method comprising: (a) providing a starting material solution comprising said protein; (b) performing cation-exchange chromatography (CEC) below pH 4 on said starting material solution; and (c) performing size-exclusion chromatography (SEC), wherein said method does not comprise use of perchloric acid.