Patent ID: 7790847
Filing Date: 2010-09-07
Classification: C12N,C12P

Abstract:
1. A translation system comprising: (a) a first unnatural amino acid that is phenylselenocysteine, (b) a first orthogonal aminoacyl-tRNA synthetase (O-RS), wherein the O-RS is at least 90% identical to SEQ ID NO: 6 and comprises: a Trp at a position corresponding to position 32 of SEQ ID NO: 6 and a Gly at a position corresponding to position 70 of SEQ ID NO:6; and, wherein the first O-RS can aminoacylate a reference tRNA comprising SEQ ID NO: 1 (MjtRNA-Tyr (c) a first orthogonal tRNA (O-tRNA), wherein the O-tRNA is at least 90% identical to SEQ ID NO: 1; and wherein the O-tRNA can be aminoacylated with the phenylselenocysteine by a reference O-RS comprising SEQ ID NO: 6; wherein said first O-RS functions to preferentially aminoacylate said first O-tRNA with said phenylselenocysteine.