Patent ID: 6228344
Filing Date: 2001-05-08
Classification: A61K

Abstract:
A method of measuring physiological function of a group of cells in a patient's body selected from the group consisting of renal or hepatic cells comprising the steps of:a) selecting a detectable agent which emits an electromagnetic emission, said agent being selectively removed from a body fluid in said patient's body by said group of cells in said patient's body, and wherein said emission occurs in said body fluid in said patient's body;b) introducing said agent into said body fluid of a patient, which body fluid contacts said group of cells in said patient's body, and wherein said emission occurs in said body fluid;c) measuring said emission from a body portion in said patient's body through which said body fluid passes wherein said emission results from agent not yet removed from said body fluid at a time of said measuring; andd) determining said physiological function based on measurement of said emission,wherein said agent is a dye conjugated to a physiologically acceptable polyanionic carrier to form a dye-polyanionic carrier conjugate which fluoresces at a first wavelength upon being irradiated with light of a second wavelength and wherein said dye-polyanionic carrier conjugate is selected from the group consisting of:Fluorescein-polyaspartic acid,Fluorescein-polyglutamic acid,Fluorescein-polyacrylic acid,Fluorescein-polynucleotides,Fluorescein-polynitrophenylalanine,Fluorescein-polydinitrophenylalanine,Fluorescein-polytrinitrophenylalanine,Fluorescein-polysulfonylphenylalanine,Fluorescein-polydisulfonylphenylalanine,Fluorescein-polytrisulfonylphenylalanine,Fluorescein-polymalonate,Fluorescein-polyglutarate,Fluorescein-polyglycolate,Bis(hexanoic acid)indocyanine green-polyaspartic acid,Bis(hexanoic acid)indocyanine green-polyglutamic acid,Bis(hexanoic acid)indocyanine green-polyacrylic acid,Bis(hexanoic acid)indocyanine green-polynucleotides,Bis(hexanoic acid)indocyanine green-polynitrophenylalanine,Bis(hexanoic acid)indocyanine green-polydinitrophenylalanine,Bis(hexanoic acid)indocyanine green-polytrinitrophenylalanine,Bis(hexanoic acid)indocyanine green-polysulfonylphenylalanine,Bis(hexanoic acid)indocyanine green-polydisulfonylphenylalanine,Bis(hexanoic acid)indocyanine green-polytrisulfonylphenylalanine,Bis(hexanoic acid)indocyanine green-polysuccinate,Bis(hexanoic acid)indocyanine green-polymalonate,Bis(hexanoic acid)indocyanine green-polyglutarate,Bis(hexanoic acid)indocyanine green-polyglycolate,Bis(propanoic acid)indocyanine green-polyaspartic acid,Bis(propanoic acid)indocyanine green-polyglutamic acid,Bis(propanoic acid)indocyanine green-polyacrylic acid,Bis(propanoic acid)indocyanine green-polynucleotides,Bis(propanoic acid)indocyanine green-polynitrophenylalanine,Bis(propanoic acid)indocyanine green-polydinitrophenylalanine,Bis(propanoic acid)indocyanine green-polytrinitrophenylalanine,Bis(propanoic acid)indocyanine green-polysulfonylphenylalanine,Bis(propanoic acid)indocyanine green-polydisulfonylphenylalanine,Bis(propanoic acid)indocyanine green-polytrisulfonylphenytalanine,Bis(propanoic acid)indocyanine green-polysuccinate,Bis(propanoic acid)indocyanine green-polymalonate,Bis(propanoic acid)indocyanine green-polyglutarate,Bis(propanoic acid)indocyanine green-polyglycolate,Bis(benzothiazole)squaraine-polyaspartic acid,Bis(benzothiazole)squaraine-polyglutamic acid,Bis(benzothiazole)squaraine-polyacrylic acid,Bis(benzothiazole)squaraine-polynucleotides,Bis(benzothiazole)squaraine-polynitrophenylalanine,Bis(benzothiazole)squaraine-polydinitrophenylalanine,Bis(benzothiazole)squaraine-polytrinitrophenylalanine,Bis(benzothiazole)squaraine-polysulfonylphenylalanine,Bis(benzothiazole)squaraine-polydisulfonylphenylalanine,Bis(benzothiazole)squaraine-polytrisulfonylphenylalanine,Bis(benzothiazole)squaraine-polysuccinate,Bis(benzothiazole)squaraine-polymalonate,Bis(benzothiazole)squaraine-polyglutarate,Bis(benzothiazole)squaraine-polyglycolate,Bis(trihydroxyphenyl)squaraine-polyaspartic acid,Bis(trihydroxyphenyl)squaraine-polyglutamic acid,Bis(trihydroxyphenyl)squaraine-polyacrylic acid,Bis(trihydroxyphenyl)squaraine-polynucleotides,Bis(trihydroxyphenyl)squaraine-polynitrophenylalanine,Bis(trihydroxyphenyl)squaraine-polydinitrophenylalanine,Bis(trihydroxyphenyl)squaraine-polytrinitrophenylalanine,Bis(trihydroxyphenyl)squaraine-polysulfonylphenylalanine,Bis(trihydroxyphenyl)squaraine-polydisulfonylphenylalanine,Bis(trihydroxyphenyl)squaraine-polytrisulfonylphenylalanine,Bis(trihydroxyphenyl)squaraine-polysuccinate,Bis(trihydroxyphenyl)squaraine-polymalonate,Bis(trihydroxyphenyl)squaraine-polyglutarate, andBis(trihydroxyphenyl)squaraine-polyglycolate;wherein prior to said measuring step, said body portion through which said body fluid passes is irradiated with said light of said second wavelength, causing said dye-polyanionic carrier conjugate to fluoresce at said first wavelength;wherein said emission being measured is fluorescence of said dye-polyanionic carrier conjugate at said first wavelength; andwherein said determination of physiological function is based on measurement of fluorescence of said first wavelength.