Patent ID: 6518020
Filing Date: 2003-02-11
Classification: C12Q,Y10S

Abstract:
A method to detect and distinguish between Haemobartonella felis Ohio and Haemobartonella felis California comprising:a) amplifying nucleic acids in a test sample by conducting a polymerase chain reaction using a single pair of primers that specifically amplifies a region of the Haemobartonella felis Ohio 16S gene comprising nucleic acid sequence SEQ ID NO: 3 and a sequence fully complementary to SEQ ID NO: 4 and a region of the Haemobartonella felis California 16S gene comprising nucleic acid sequence SEQ ID NO:3 and a sequence fully complementary to SEQ ID NO: 4, wherein said region of the H. felis Ohio 16S gene is approximately 25 base pairs shorter than said region of the H. felis California 16S gene, such that an H. felis Ohio amplification product produced using said pair of primers is shorter than an H. felis California amplification product produced using said primers; and b) detecting the presence of and determining the length of amplification products produced by said amplifying step a), wherein the presence and length of amplification products is indicative of the presence of H. felis Ohio and/or H. felis California in said test sample, and wherein the absence of amplification products is indicative of the absence of both H. felis Ohio and H. felis California in said test sample.