Patent ID: 8652462
Filing Date: 2014-02-18
Classification: A61K,A61P,C07K,C12N

Abstract:
1. A method of inhibiting allograft rejection in a subject in need thereof, the method comprising: providing antigen presenting cells (APCs) from the subject; loading a peptide fragment of an α or β chain of a preselected major histocompatibility complex (MHC) class II protein from the subject onto the subject's APCs outside the body of the subject, wherein the loading comprises introducing into the subject's APCs an expression vector encoding one or both of the α or β chain of the preselected MHC class II protein or synthesizing the peptide fragment and supplying the synthesized peptide fragment to the subject's APCs, and wherein the peptide fragment is presented on MHC class II proteins of the subject's APCs; and introducing the loaded APCs into the subject in an amount sufficient to activate autogenous peripheral T regulatory cells and suppress alloreactive autogenous cytotoxic T cells in the subject, thereby inhibiting allograft rejection in the subject.