Patent ID: 8932859
Filing Date: 2015-01-13
Classification: C12P

Abstract:
1. A method of engineering red fluorescent protein non wild-type variants having an altered emission peak, comprising the steps of: a) introducing a polynucleotide encoding the red fluorescent protein of SEQ ID NO: 1 under control of an inducible promoter into at least one cell of a population of cells which support somatic hypermutation (SHM); b) inducing expression of said red fluorescent protein of SEQ ID NO: 1, whereby said polynucleotide is mutated in one or more mutation rounds by SHM, wherein said mutation round provides a multiplicity of non wild-type variants of said red fluorescent protein of SEQ ID NO: 1; and c) selecting a cell expressing red fluorescent protein variant having an altered emission peak, as compared to the emissions peak of the red fluorescent protein of SEQ ID NO: 1, and having the sequence of SEQ ID NO: 3 or 5.