Patent ID: 8093452
Filing Date: 2012-01-10
Classification: C12N,C12Q,G01N

Abstract:
1. A plant cell wherein said cell is transformed with a nucleic acid molecule comprising an expression cassette which cassette comprises a nucleic acid molecule selected from the group consisting of: i) a nucleic acid molecule comprising a nucleic acid sequence as represented in SEQ ID NO: 1; ii) a nucleic acid molecule which hybridises under stringent hybridisation conditions comprising hybridization at a temperature of 65-70° C. for 16 hours in 5-6X SSC to the complement of the nucleic acid molecule in (i) and which comprises a nucleotide sequence that encodes a polypeptide that has phospholipase and/or triacylglycerol lipase activity; and iii) a nucleic acid molecule that comprises a nucleotide sequence that encodes a polypeptide comprising an amino acid sequence as represented in SEQ ID NO: 2, or a variant polypeptide which is modified by addition, deletion or substitution of at least one amino acid residue, said variant polypeptide having an amino acid sequence with at least 90% identity to the amino acid sequence represented in SEQ ID NO:2, wherein said polypeptide or variant polypeptide has phospholipase and/or triacylglycerol lipase activity; wherein said cassette is adapted such that both sense and antisense nucleic acid molecules are transcribed from said cassette.