Patent ID: 6773706
Filing Date: 2004-08-10
Classification: A61P,C07K,C12N

Abstract:
A substantially purified sortase-transamidase enzyme from a Gram-positive bacterium, the enzyme catalyzing a reaction that covalently cross-links the carboxyl terminus of a protein having a sorting signal to the peptidoglycan of a Gram-positive bacterium, and comprisingM-R-M-K-R-F-L-T-I-V-Q-I-L-L-V-V-I-I-I-I-F-G-Y-K- I-V-Q-T-Y-I-E-D-K-Q-E-R-A-N-Y-E-K-L-Q-Q-K-F-Q-M- L-M-S-K-H-Q-A-H-V-R-P-Q-F-E-S-L-E-K-I-N-K-D-I-V- G-W-I-K-L-S-G-T-S-L-N-Y-P-V-L-Q-G-K-T-N-H-D-Y-L- N-L-D-F-E-R-E-H-R-R-K-G-S-I-F-M-D-F-R-N-E-L-K-I- L-N-H-N-T-I-L-Y-G-H-H-V-G-D-N-T-M-F-D-V-L-E-D-Y- L-K-Q-S-F--Y-E-K-H-K-I-I-E-F-D-N-K-Y-G-K-Y-Q-L- Q-V-F-S-A-Y-K-T-T-T-K-D-N-Y-I-R-T-D-F-E-N-D-Q-D- Y-Q-Q-F-L-D-E-T-K-R-K-S-V-I-N-S-D-V-N-V-T-V-K-D- K-I-M-T-L-S-T-C-E-D-A-Y-S-E-T-T-K-R-I-V-V-V-A-K- I-I-K-V-S (SEQ ID NO:38).