Patent ID: 8526105
Filing Date: 2013-09-03
Classification: G02B,G06T

Abstract:
1. An apparatus for coherent anti-Stokes Raman (CARS) microscopy comprising: an object plane on which is disposed a first surface of a planar substrate, wherein the substrate is characterized by a homogeneous refractive index and a surface normal, wherein the substrate is arranged to support an object; a first optical system disposed to provide an illumination of the object plane, the illumination characterized by two substantially coincident coherent beams with wavelengths λ a second optical system having an optical axis disposed at one of a plurality of center wave vectors from about 0 to about 2πn a third optical system disposed between the first optical system and an entrance aperture of a first optical element of the second optical system to provide interferometric reintroduction of the illumination as a reference beam at an angular frequency of 2ω an electronic image device disposed at the image plane of the second optical system and operable to respond linearly to a local optical intensity and transfer a local optical intensity map across the image plane as a sub-image to a signal processor device in electronic form; one or more devices operable to adjust the first, the second, and the third optical systems to collect sub-images for different pairs of the pluralities of incident illumination wave vectors from the first optical system and collection angles from the second optical system so as to sequentially obtain a plurality of sub-images corresponding to a plurality of regions of spatial frequency space, wherein the signal processor device is operable to sequentially receive the electronic form of the sub-images and manipulate the sub-images to correct for distortions and alterations introduced by the optical configuration, store, and combine the plurality of sub-images corresponding to the plurality of regions of spatial frequency space to create a composite image.