Patent ID: 6589733
Filing Date: 2003-07-08
Classification: A61K,C12N,C12Q

Abstract:
A method of preparing a composition which comprises:(a) contacting a sample which contains a predefined number of eucaryotic cells with a chemical to be tested, each cell comprising a DNA construct comprising: (i) a modulatable transcriptional regulatory sequence of a gene-of-interest, (ii) a promoter, and (iii) a reporter gene that produces a detectable signal, coupled to, and under the control of, the promoter, under conditions wherein the chemical if capable of acting as a transcriptional modulator of the gene-of-interest, causes a detectable signal to be produced by the reporter gene; (b) quantitatively determining the amount of the signal produced in (a); (c) comparing the amount of signal determined in (b) with the amount of signal produced and detected in the absence of any chemical being tested or with the amount of signal produced and detected upon contacting the sample in (a) with other chemicals, thereby identifying the test chemical as a chemical which causes a change in the amount of detectable signal produced by the reporter gene; and (d) admixing a homolog or analog of the chemical from step (c) with a carrier, thereby preparing the composition.