Patent ID: 8986951
Filing Date: 2015-03-24
Classification: A61K,A61P,C07K,C12N

Abstract:
1. A mutein of human tear lipocalin having binding affinity to Interleukin 4 (IL 4) receptor alpha, wherein with resect to the amino acid sequence of mature human tear lipocalin said mutein comprises a set of amino acid substitutions selected from the group consisting of: (1) Arg 26→Ser; Asn 32→Tyr; Met 55→Leu; Leu 56→Gln; and Ser 58→Lys; (2) Arg 26→Pro; Asn 32→Tyr; Glu 34→Ser; Met 55→Ala; Leu 56→Gln; and Glu 63→Lys; (3) Arg 26→Leu; Asn 32→Phe; Glu 34→Trp; Met 55→Ala; Ser 58→Ile; and Glu 63→Ser; (4) Arg 26→Ser; Asn 32→Tyr; Glu 34→Val; Met 55→Ala; Leu 56→Ala; Ser 58→Ile; and Glu 63→Ser; (5) Arg 26→Ser; Asn 32→Val; Glu 34→Asn; Met 55→Ala; Leu 56→Gln; Ser 58→Lys; and Glu 63→Lys; (6) Arg 26→Asn; Asn 32→Lys; Glu 34→Asp; Met 55→Ala; Leu 56→His; Ser 58→Arg; and Glu 63→Gln; (7) Arg 26→Tyr; Asn 32→Tyr; Glu 34→His; Met 55→Ala; Leu 56→His; Ser 58→Ala; and Glu 63→Lys; (8) Arg 26→Lys; Asn 32→Tyr; Glu 34→Arg; Met 55→Ala; Leu 56→Lys; Ser 58→Asn; and Glu 63→Pro; and (9) Arg 26→Glu; Asn 32→His; Glu 34→Gly; Met 55→Ala; Leu 56→Met; Ser 58→Leu; and Glu 63→Lys.