Patent ID: 8192976
Filing Date: 2012-06-05
Classification: C12N,C12P

Abstract:
1. A metabolically engineered host cell for the production of a α,ω difunctional Cn alkane from a α-ketoacid, wherein α and ω terminal functional groups are selected from the group of —OH, —COOH and —NH3 and wherein n is an integer in the range of 4 to 8, wherein the metabolically engineered host cell is genetically modified with one or more nucleic acid sequences encoding: a set of at least two enzymes of a biosynthetic pathway, comprising a recombinant α-ketoacid decarboxylase capable of using the α-ketoacid as a substrate and at least a recombinant aldehyde dehydrogenase, a recombinant alcohol dehydrogenase, a recombinant aminotransferase, or a combination thereof, or a set of at least two enzymes of a biosynthetic pathway, comprising a recombinant 2-aminotransferase capable of using the α-ketoacid as a substrate and at least a recombinant aminodecarboxylase, a recombinant reductase, a recombinant dehydrogenase, a recombinant decarboxylase, a recombinant aldehyde dehydrogenase, a recombinant alcohol dehydrogenase, a recombinant aminotransferase, a recombinant aminoaldehyde dehydrogenase, a recombinant 2-aminoaldehyde mutase, a recombinant dehydratase or a combination thereof, or a set of at least two enzymes of a biosynthetic pathway, comprising a recombinant dehydrogenase capable of using the α-ketoacid as a substrate and at least a recombinant dehydratase, a recombinant aldehyde dehydrogenase, a recombinant alcohol dehydrogenase, a recombinant 1-aminotransferase, or a combination thereof.