Patent ID: 8889355
Filing Date: 2014-11-18
Classification: C12Q

Abstract:
1. A method for detecting a target nucleic acid in a sample, comprising: a. contacting the sample with at least one set of at least three oligonucleotide probes for a time and under conditions suitable to form an annealed product, wherein the at least one set of at least three oligonucleotide probes comprises at least one first chimeric oligonucleotide probe, at least one medial oligonucleotide probe, and at least one second chimeric oligonucleotide probe; b. contacting the annealed product with a polypeptide having double-strand dependent ligase activity for a time and under conditions suitable to form a ligated product; and c. detecting the target nucleic acid in the sample by detecting the ligated product, or a surrogate thereof.