Patent ID: 6649339
Filing Date: 2003-11-18
Classification: A61P,C12Q

Abstract:
A method for producing a pool of quality assured biological samples comprising:(a) testing a screening pool for the presence of a parvovirus genome equivalent, wherein the testing is performed by a first nucleic acid amplification process that has a detection limit (DL-1) for parvovirus, wherein the screening pool comprises collected aliquots from a plurality of biological samples, and wherein the testing determines whether the screening pool has a content of genome equivalents that exceeds a preset limit; (b) forming screening subpools from the screening pool of (a) that have a content of genome equivalents that exceed the preset limit; wherein the screening subpool is comprised of aliquots from a portion of the plurality; (c) testing the subpools of (b) for the presence of a parvovirus genome equivalent, wherein the testing is performed by a second nucleic acid amplification process that has a detection limit (DL-2) for parvovirus, wherein DL-1 is lower than DL-2; (d) eliminating samples corresponding to subpools that have a genome equivalent content that exceeds DL-2; and (e) combining the remaining samples to produce the pool of quality assured biological samples.