Patent ID: 6080846
Filing Date: 2000-06-27
Classification: A61K,C07K,C12N,G01N

Abstract:
A recombinant DNA havinga) a first nucleotide sequence encoding an amino acid sequence for a B epitope, which is a major neutralization epitope, of env glycoprotein of a virus selected from the group consisting of a human immunodeficiency virus type 1 (HIV-1), human immunodeficiency virus type 2 (HIV-2), simian immunodeficiency virus (SIV), human T-cell lymphotropic virus type 1 (HTLV-I), and human T-cell lymphotropic virus type II (HTLV-II), wherein said B epitope is selected from the group consisting of:(1) NTRKR IRIQRGPGRA FVTIGK-IGN; - (2) NTRKK IRIQRGPGRA FVTIGK-IGN; - (3) NTRGS IRIQRGPGRA FVTIGK-IGN; - (4) NTRKS IYI--GPGRA FHTTGRIIGD; - (5) NVRRS LSI--GPGRA FRTRE-IIGI; - (6) NTRRG IHF--GPGQA LYTTGIV-GD; - (7) NTRQR TPI--GLGQS LYTTRSR-SI; - (8) NTRKS ITK--GPGRV IYATGQIIGD; - (9) NTRKR ITM--GPGRV YYTTGQIIGD; - (10) DKRQS TPI--GLGQA LYTTRGRTKI; - (11) DKKIR QSIRIGPGKV FYAKGG---I; - (12) NTKKG IAI--GPGRT LYAREKIIGD; - (13) HTRKR VTL--GPGRV WYTTGEILGN; - (14) NTRRG SHF--GPGQA LYTTGIVGDI; - (15) KITSRQQTPI--GLQA LYTTRIKGDI; - (16) NVRRR HIHI-GPGRA FYTGEIRNI; - (17) NTRQS TPI--GLGQA LYTTRTKSI; - (18) NTTRS IHI--GPGRA FYATGDIIGTI; - (19) NKRKR IHI--GPGRA FYTTKNIIGDI; - (20) TRPNNNTRKR IRIQRGPGRA FVTIGK-IGN M-RQAH; - (21) TRPNN TRKS IRIQRGPGRA FVTIGK-IGN M-RQAH; - (22) TRPNNNTRKK IRIQRGPGRA FVTIGK-IGN M-RQAH; - (23) TRPNNNTRGS IRIQRGPGRA FVTIGK-IGN M-RQAH; - (24) TRPNNNTRKS IYI--GPGRA FHTTGRIIGD-IRKAH; - (25) TRPYNNVRRS LSI--GPGRA FRTRE-IIGI IRQAH; - (26) TRPGNNTRRG IHF--GPGQA LYTTGIV-GD-IRRAY; - (27) ARPYQNTRQR TPI--GLGQS LYTTRSR-SI-IGQAH; - (28) TRPNNNTRKS ITK--GPGRV IYATGQIIGD-IRKAH; - (29) TRPNNNTRKR ITM--GPGRV YYTTGQIIGD-IRRAH; - (30) TRPGSDKRQS TPI--GLGQA LYTTRGRTKI-IGQAH; - (31) TRPGSDKKIT QSIRIGPGKV FYAKGG---I-TGQAH; - and - (32) TRPNNNTKKG IAI--GPGRT LYAREKIIGD-IRQAH; - and b) a second nucleotide sequence encoding an amino acid sequence for a T epitope of nef protein of HIV-1 BRU, wherein said T epitope is selected from the group consisting of:(1) GMDDP EREVL EWRFD SRLAF HHVAR ELKPE YFKNC; - (2) CYKLV PVEPD KVEEA NKGEN TSKKH PVSLH GMDDP; - (3) EREVL EWRFD SRLAF HHVAR ELHPE YFKNC; - (4) DSRLA FHHVA RELHP EYFKN C; - (5) CGYFP DWQNY TPGPG VRYPL TFGWC YKLVP VEPDK; - and; - (6) VEEAN KGENT SLLHP V.