Patent ID: 7115798
Filing Date: 2006-10-03
Classification: A61K,C07K,C12N

Abstract:
1. A method of conditionally activating a transgene in a plant comprising: 1) providing constructs comprising: wherein: (i) P1 is a first germline promoter; (ii) R1 is a first recombinase coding sequence and 3′ region; (iii) RS1 is a first recombinase site responsive to a first recombinase; (iv) P2 is a second floral common germline promoter; (v) RS2 is a second recombinase site responsive to a second recombinase; (vi) STP is a stop fragment; (vii) R2 is a second recombinase coding sequence and 3′ region; (viii) TG1 is a first transgene sequence and 3′ region; (ix) TG2 is a second transgene sequence and 3′ region; (x) P3 is a third promoter which is not expressed in floral tissue and; (xi) P4 is a fourth promoter which is not expressed in floral tissue and; wherein P1, P2, P3 and P4 are operably linked to their down stream elements and wherein TG1 and TG2 are different trait transgenes and wherein P3 and P4 are activated in a second generation plant; 2) providing a first and second plant selected from the group consisting of: 3) crossing the first and second plants to produce a first generation plant wherein conditional expression of the first recombinase coding sequence (R1) under the control of the P1 promoter in the common germline of the first generation, excises the stop fragment from the second recombinase element allowing expression of the second recombinase coding sequence and 3′ region (R2) under the control of P2 promoter, which recombinase, in turn, excises the stop fragments from the third and fourth recombinase elements, permitting expression of the trait gene(s) TG1 and TG2 under the control of P3 and P4 promoter, respectively, in the second generation.