Patent ID: 6376210
Filing Date: 2002-04-23
Classification: C12Q,G01N

Abstract:
A method for assaying homocysteine (Hcy) in a sample, which method comprises:a) contacting the sample with a mutant S-adenosylhomocysteine (SAH) hydrolase comprising the amino acid sequence set forth in SEQ ID No. 1 and comprising one or more mutations selected from the group consisting of Phe302 to Ser (F302S), Lys186 to Ala (K186A), His301 to Asp (H301D), His353 to Ser (H353S), Arg343 to Ala (R343A), Asp190 to Ala (D190A), Phe82 to Ala (F82A), Thr157 to Leu (T157L), Asn181 to Asp (N181D), deletion of Tyr432 (&Dgr;432) and a double mutation of Arg431 to Ala (R431 A) and Lys426 to Arg (K426R); and b) detecting binding between Hcy, S-adenosylhomocysteine (SAH) or adenosine with said mutant SAH hydrolase, whereby the presence or amount of Hcy in the sample is assessed.