Patent ID: 8921113
Filing Date: 2014-12-30
Classification: B01D,C09K,G01N,Y10T

Abstract:
1. A buffer kit comprising: a) a first eluent solution including at least four buffer salts where at least three of the four buffer salts are a monovalent buffer salt, have a net negative charge or a net neutral zwitterionic charge over a pH range of about 6 to about 10, and include a sulfonate group and an amine, where the first eluent solution has a first pH of about 6 and a total buffer salt concentration of greater than about 25 millimolar; b) a second eluent solution including at least four buffer salts where at least three of the four buffer salts are a monovalent buffer salt, have a net negative charge or a net neutral zwitterionic charge over a pH range of about 6 to about 10, and include a sulfonate group and an amine, where the second eluent solution has a second pH of about 10 and a total buffer salt concentration of greater than about 25 millimolar; whereby the buffer kit provides a linear pH gradient, based on a function of time and pH values, that forms an approximately straight line for at least a pH range of about pH 6 to about pH 10.