Patent ID: 6489096
Filing Date: 2002-12-03
Classification: C12Q,G16B

Abstract:
A method for analyzing a sample nucleic acid sequence comprising:providing a plurality of hybridization intensities of probes including exposing said probes to said sample nucleic acid sequence, said probes including pairs of perfect match and mismatch probes, each pair including a perfect match probe perfectly complementary to a subsequence of said nucleic acid sequence and a mismatch probe having at least one base mismatch with said particular subsequence; applying a non-linear low-pass filter to said plurality of hybridization intensities, wherein applying the non-linear filter includes averaging hybridization intensities of perfect match probes over subsequences aligned to a particular base position and subsequences aligned to surrounding base positions, excluding outlying hybridization intensities; and comparing pairs of relative hybridization intensities, wherein comparing pairs of said relative hybridization intensities determines an expression of a gene specified by said sample nucleic acid sequence.