Patent ID: 9115377
Filing Date: 2015-08-25
Classification: C12N,C12P,C12R

Abstract:
1. A method for vanillin production using cells of Amycolatopsis sp. strain (zhp06) or its mutant strains, comprising the steps of: (a) addition of (b) purification of vanillin from said biotransformation solution after 5-12 hours of said conversion reaction of ferulic acid to vanillin, wherein said purification is performed by adding 5-50% (w/v) of macroporous absorbent resin to said biotransformation solution, wherein vanillin is extracted from the solution using said absorbent resin which is then eluted with acetic ether or alcohol at 35-40° C. to produce an eluate solution containing vanillin; and wherein said eluate solution is dried by anhydrous sodium sulfate and further dried by vacuum evaporation to produce a concentrated extract containing 200-230 g/L of vanillin; and subsequently, said concentrated extract is settled at 4° C. for vanillin crystallization thereby purifying vanillin from the biotransformation solution.