Patent ID: 9206468
Filing Date: 2015-12-08
Classification: C12Q

Abstract:
1. A one-step method of eluting DNA from a blood sample, consisting of adding a one-step elution buffer to the blood sample to form a mixture, wherein the one-step elution buffer consists of 5 to 22.5 mM potassium in the form of KOH, KCl, or a combination thereof, and 7.5 to 30 mM of a base having a buffering range of 7.0 to 9.5, wherein the pH of the one-step elution buffer is 9 to 13, heating the mixture at 90° C. to 99° C. for a time sufficient to elute the DNA from the blood sample to form an eluted DNA solution, and optionally cooling the eluted DNA solution at a temperature of at least 4° C. for at least 5 minutes, wherein the eluted DNA solution is suitable for direct use in an enzymatic DNA amplification reaction.