Patent ID: 8962318
Filing Date: 2015-02-24
Classification: A61P,C12N,C12Q,G01N

Abstract:
1. A method of establishing, from an embryonic stem (ES) cell, a mesenchymal progenitor cell line comprising mesenchymal stem cells (MSCs), the method comprising: (a) propagating an ES cell in the absence of co-culture with a feeder cell layer, in a serum free medium comprising FGF2, (b) selecting mesenchymal stem cells from the propagated cells based on expression of one or more cell surface markers, and (c) growing the mesenchymal stem cells selected in step (b) in medium comprising serum; thereby establishing a mesenchymal progenitor cell line comprising MSCs, wherein the mesenchymal progenitor cell line maintains self-renewal without transformation, and wherein the mesenchymal progenitor cell line is lineage restricted compared to the ES cell.