Patent ID: 8105831
Filing Date: 2012-01-31
Classification: A61K,A61P,C07K,C12N

Abstract:
1. A recombinant targeting parvoviral vector comprising: a) a targeting nucleotide sequence that provides for homologous recombination with a target nucleotide sequence centromeric to an entire human leukocyte antigen (HLA) locus; b) a selectable marker that provides for positive selection for integration of the targeting nucleotide sequence into the chromosome and negative selection for homozygosity at the entire HLA locus, wherein the selectable marker is a nucleic acid comprising a nucleotide sequence encoding a fusion polypeptide that provides for both positive and negative selections, and wherein the selectable marker is located within the targeting nucleotide sequence; and c) at least one inverted terminal repeat, or a functional equivalent thereof, flanking the targeting nucleotide sequence.