Patent ID: 6090288
Filing Date: 2000-07-18
Classification: B01D,B01J,C07K,G01N

Abstract:
Process for separating off a peptide or a nucleic acid, comprising contacting an aqueous solution containing the peptide or the nucleic acid with an anion exchanger (I) under such conditions that the anion exchanger (I) binds substances in the solution so that the desired peptide or nucleic acid is detached from at least one substance in the solution, characterized in thata. the anion exchanger (I) exhibits ligands, whichi. contain a primary, secondary or tertiary amino group andii. are covalently bound, through an organic bridge, to an organic polymer (matrix),b. on a carbon atom at a distance of 2 or 3 atoms away from an amino nitrogen there is a hydroxyl group or a primary, secondary or tertiary amino group, andc. the maximum elution ionic strength in the pH range 2-14 for at least one of the proteins transferrin, ovalbumin 1, ovalbumin 2, .beta.-lactoglobulin 1 and .beta.-lactoglobulin 2 on the anion exchanger (I) is at least 125% of the required elution ionic strength required for a quaternary anion exchanger (II) with Q-group ((CH.sub.3).sub.3 N.sup.+ --; the same matrix, the same coupling group from the quaternary nitrogen and in towards the matrix, the same level of ligand as for the anion exchanger (I) and the elution ionic strength measured at the same pH for anion exchangers I and II,with the provisos that chromatofocusing and separation on anion exchangers prepared by coupling of triethanol amine to cellulose via reaction with epoxide are not included.