Patent ID: 8119388
Filing Date: 2012-02-21
Classification: A61P,C12N

Abstract:
1. A cell culture composition comprising non-tumorigenic and adherent Madin Darby Canine Kidney (MDCK) cells that have been adapted to grow in a serum-free medium comprising Taub's medium, lipids and wheat hydrolysate, which Taub's medium comprises a 50:50 mixture of DMEM and Ham's F12, 4.5 grams per liter glucose, 4mM L-glutamine, 5 micrograms per milliliter insulin, 5 micrograms per milliliter transferrin, 5picomolar triiodothyronine, 50 nanomolar hydrocortisone, 25 nanograms per milliliter prostaglandin El and 10 nanomolar sodium selenite.