Patent ID: 6541194
Filing Date: 2003-04-01
Classification: B01L,G01N

Abstract:
A method for the detection of chemical species known to inhibit a chemiluminescent reaction in an assay for an analyte of interest, the method comprising the steps of:a.) sampling a surface with a sampling device, wherein the surface is suspected of contamination by the presence of cellular material; b.) contacting the sample with a reagent solution comprising a known amount of the analyte of interest or a chemical species associated with the analyte of interest; c.) reacting the reagent solution with a reactant mixture capable of producing chemiluminescence through reaction with the analyte of interest or the chemical species associated with the analyte of interest present in the reagent solution; d.) measuring the amount of chemiluminescent emission from the reaction; e.) comparing the amount of measured chemiluminescent emission from the reaction to an expected amount of chemiluminescent emission based on the known amount of the analyte of interest or the chemical species associated with the analyte of interest originally in the reagent solution; and f.) determining the presence of inhibitory species when the measured chemiluminescent emission is less than the expected amount of chemiluminescent emission.