Patent ID: 8828687
Filing Date: 2014-09-09
Classification: A61K,A61P,C07K,C12N,Y02A

Abstract:
1. A method for the production of recombinant vector virus, wherein the vector virus comprises nucleotide sequences encoding at least one heterologous protein, or fragments thereof, said method comprising the following steps: (a) providing at least one heterologous nucleotide sequence, (b) providing a vector virus sequence, wherein said vector virus sequence comprises nucleotide sequences encoding the NS1 protein and the E protein sequence of a (c) cloning the heterologous sequence of step (a) into the vector virus sequence, wherein the insertion of the heterologous sequence occurs in the E/NS1 intergenic region, further wherein the nucleotides present at the 5′ end of the NS1 gene and the whole or part of the domains of stem and anchor of the E protein are present at either end of the heterologous sequence; wherein when the heterologous protein is expressed it will be processed normally by a host cell and can induce an immune response.