Patent ID: 6344319
Filing Date: 2002-02-05
Classification: C12Q,G16B,Y02A,Y10T

Abstract:
A method of determining a copy number of a nucleotide sequence of interest in genomic DNA, comprising:(a) providing a surface on which genomic DNA has been aligned using a molecular combing technique; (b) contacting the aligned genomic DNA with a first labeled probe of length (lt), wherein the first labeled probe is specific for a control genomic sequence whose copy number is known, and with a second labeled probe of length (lc), wherein the second labeled probe is specific for the nucleotide sequence of interest; (c) detecting hybridization signals between the first labeled probe and the control genomic sequence and between the second labeled probe and the nucleotide sequence of interest; (d) measuring the total length of the hybridization signals that correspond to the first labeled probe (Lt) and the second labeled probe (Lc); (e) calculating the copy number of the nucleotide sequence of interest using the ratios: Nt=Lt/lt and Nc=Lc/lc, â€ƒwherein Nt corresponds to the copy number of the control genomic sequence and Nc corresponds to the copy number of the sequence of interest.