Patent ID: 6153419
Filing Date: 2000-11-28
Classification: C12N,C12Q,C12Y,G01N,Y10S

Abstract:
A method for detecting a disease state in a mammal, comprising the steps of:(a) quantitatively determining 1,5-anhydroglucitol in a biological sample, comprising the steps of:(i) selecting an enzyme having activity that is inhibited by 1,5-anhydroglucitol in a concentration-dependent manner, wherein said enzyme does not use 1,5-anhydroglucitol as a substrate and has a Ki value of 0.33 mM or less for 1,5-anhydroglucitol,(ii) mixing the biological sample with said enzyme, wherein the biological sample is cerebrospinal fluid, blood plasma, serum or urine,(iii) thereafter measuring activity of the enzyme, andcomparing measured enzyme activity with a calibration curve obtained from known amounts of 1,5-anhydroglucitol; and(b) determining a disease state of said mammal by comparison of changes in the 1,5-anhydroglucitol level.