Patent ID: 6746669
Filing Date: 2004-06-08
Classification: A61K,A61P,C07K

Abstract:
A method for down-regulating interleukin 5 (IL5) activity in an anal in vivo, the method comprising:administering an immunogenically effective amount of at least one modified IL5 polypeptide to the animal, wherein said modified IL5 polypeptide comprises at least one modification in the IL5 polypeptide and wherein said modification comprises introduction of at least one foreign, immunodoninant T helper lymphocyte epitope (TH epitope), results in a specific reaction between the modified IL5 polypeptide and an antiserum raised against the animal's IL5 polypeptide, and is made in at least one of loops 1-3 or in the amino acid residues C-terminal to helix D of human and murine IL5, wherein the C-terminal of human IL5 corresponds to residues 110-115 of SEQ ID No. 1, loop 1 of human IL5 corresponds to residues 32-44 of SEQ ID NO. 1, loop 2 of human IL5 corresponds to residues 58-64 of SEQ ID No. 1 and loop 3 of human IL5 corresponds to residues 85-92 of SEQ ID NO. 1 and wherein the C-terminal of murine IL5 corresponds to residues 108-113 of SEQ ID NO. 12, loop 1 of murine IL5 corresponds to residues 30-42 of SEQ ID NO. 12, loop 2 of murine IL5 corresponds to residues 56-62 of SEQ ID No. 12 and loop 3 of murine IL5 corresponds to residues 83-90 of SEQ ID No. 12, whereby immunization of the animal with the modified IL5 polypeptide induces production of antibodies against the animal's IL5 polypeptide.