Patent ID: 7863022
Filing Date: 2011-01-04
Classification: C12Q

Abstract:
1. A method comprising the act of: amplifying a nucleic acid using one or more amplimers to form amplicons; labeling said amplicons with magnetic particles; binding the amplicons to a sensor surface via a biological molecule, wherein during the binding act, increasing collisions between the amplicons labeled with the magnetic particles and the biological molecule bound to the sensor surface by magnetic force cycling; and determining an amount of the amplicons bound to the sensor surface via magnetic detection; wherein the magnetic detection is performed to detect the amplicons with the magnetic particles at least one time during the amplifying act; wherein the magnetic force cycling includes cycling with velocity components perpendicular to the sensor surface to increase concentration near the sensor surface of the amplicons labeled with the magnetic particles and bound to the sensor surface and restrict movement of particles parallel to the sensor surface, while intermittently cycling with velocity components parallel to the sensor surface in a coordinated way with the cycling of the perpendicular components to move the concentrated amplicons labeled with the magnetic particles horizontally and in close proximity over the sensor surface to further increase the collisions.