Patent ID: 9222885
Filing Date: 2015-12-29
Classification: C12Q,G01J,G01N

Abstract:
1. A method for determining the amount of nucleic acid present in a biological sample, comprising the steps of: adding to the biological sample a single fluorophore capable of interacting with the nucleic acid wherein the fluorophore is ([N-bis-(3-dimethylaminopropyl)-amino]-4-[2,3-dihydro-3-methyl-(benzo-1,3-thiazol-2-yl)-methyllidene]-1-phenyl-quinolinium]+); measuring fluorescence intensities, I deducing the amount of nucleic acid present in the sample from the at least two measured fluorescence intensities, wherein the deducing step comprises calculating a value F according to at least one of the formulas: and determining the amount of nucleic acid in the sample from the value of F by using a calibration curve.