Patent ID: 8236552
Filing Date: 2012-08-07
Classification: C07C,C07D,C12N,C12P,C12Y,H01Q

Abstract:
1. A recombinant yeast, comprising: at least two integrated copies of a heterologous nucleic acid encoding a truncated yeast HMG-CoA reductase enzyme that converts hydroxymethylglutaryl-CoA to mevalonic acid; and, a plasmid comprising heterologous nucleic acid molecules encoding: a yeast enzyme that converts mevalonic acid to phosphomevalonic acid; a yeast enzyme that converts phosphomevalonic acid to phosphomevalonate; and, a yeast enzyme that converts phosphomevalonate to isopentenyl pyrophosphate; wherein the recombinant yeast has a decreased activity of squalene synthase relative to wild type yeast due to repression of an endogenous ERG9 gene by a heterologous repressible promoter and wherein the recombinant yeast produces farnesyl or farnesol, in an amount greater than about 11% of dry cell weight.