Patent ID: 6110713
Filing Date: 2000-08-29
Classification: C12P

Abstract:
A method for producing glutamic acid which comprises(a) culturing a biologically pure culture of a ribulose-monophosphate-pathway-utilizing, auxotrophic mutant of a strain of Bacillus methanolicus in a nutrient medium comprising vitamin B.sub.12 and wherein methanol is a primary carbon source, at a temperature of about 35.degree. C. to about 60.degree. C. until glutamic acid is produced at a concentration of at least about 5 g/l,wherein said auxotrophic mutant is obtained from a parent strain selected from the group consisting of Bacillus methanolicus MGA3, an environmental isolate having all the identifying characteristics of Bacillus methanolicus MGA3, a stable morphological mutant of Bucillus methanolicus MGA3, and a stable morphological mutant of an environmental isolate having all the identitying characteristics of Bacillus methanolicus MGA3,wherein the identifying characteristics of Bacillus methanolicus MGA3 are: rod-shape, non-motilc, Gram positive, spores at a suberminal to central position, growth at 35.degree. C. to 60.degree. C. optimum growth at 55.degree. C., growth on methanol, and a G/C content of 44% to 52%; and(b) recovering said at least about 5 g/l of glutamic acid from the nutient medium.