Patent ID: 7504261
Filing Date: 2009-03-17
Classification: B82Y,C12Q,Y10S,Y10T

Abstract:
1. A method of detecting nucleic acids in a nucleic acid sample, the method comprising: contacting a sample comprising a nucleic acid with a non-specific nucleic acid binding agent in an electrically conductive fluid medium, wherein the non-specific nucleic acid binding agent is an intercalator selected from the group consisting of YOYO (1,1′-[1,3-propanediylbis[(dimethyliminio)-3,1-propanediyl]]bis[4-[(3-methyl-2(3H)-benzoxazolylidene)methyl]]-,tetraiodide), TOTO (1-1′-[1,3-propanediylbis[(dimethyliminio)-3,1-propanediyl]]bis[4-[(3-methyl-2(3H)-benzothiazolylidene)methyl]]-,tetraiodide) and Ethidium Bromide (EtBr); contacting the sample comprising the nucleic acid bound to the non-specific nucleic acid binding agent with a nanopore, wherein the nanopore has a diameter ranging from 1.5 nm to 120 nm; and applying a voltage across the nanopore, wherein the voltage ranges from 100 mV to 1000 mV; and detecting nucleic acids by detecting an increase in current through the nanopore.