Patent ID: 6372438
Filing Date: 2002-04-16
Classification: C07K,G01N

Abstract:
A method for evaluating the ability of a compound to function as a human platelet-derived growth factor receptor (hPDGF-R) agonist comprising the steps of:obtaining transformed mammalian cells comprising a recombinant nucleic acid encoding a hPDGF-R polypeptide, wherein a recombinant hPDGF-R polypeptide is expressed from said nucleic acid; incubating a first portion of the transformed mammalian cells with the compound, wherein test cells are produced; incubating a second portion of the transformed mammalian cells without the compound, wherein control cells are produced; and comparing the amount of hPDGF-R activation in the test cells and the amount of hPDGF-R activation in the control cells, wherein increased hPDGF-R activation in the test cells compared to the control cells is correlated with the ability of the compound to function as a hPDGF-R agonist.