Patent ID: 6146838
Filing Date: 2000-11-14
Classification: G01N,Y10S,Y10T

Abstract:
A method for the detection or quantitation of Cryptosporidia in a water sample comprising:(a) filtering said water sample to obtain a retentate comprising oocysts and/or sporozoites of Cryptosporidia present in the water sample;(b) extracting said retentate in an extraction medium;(c) solubilizing, in said extraction medium, an antigen of said oocysts and/or sporozoites;(d) forming an assay mixture comprising,(i) said solubilized antigen and(ii) an antibody specific to said antigen;(e) incubating said assay mixture under conditions sufficient to permit binding of said antibody and said antigen thereby forming an antibody-antigen complex; and(f) determining the presence of said antibody-antigen complex, thereby detecting or quantitating Cryptosporidia in said water sample.