Patent ID: 8574543
Filing Date: 2013-11-05
Classification: C07B,C07K,G16B

Abstract:
1. A method comprising: using a processor, determining a protein turnover ratio in a cell associated with cancer based on a newly synthesized and a pre-existing fraction of a protein from a mass spectra of a peptide produced by enzyme digestion of the protein before and after isotope labeling, wherein the resultant spectra are presented as integrated peak heights of a corresponding mass to charge ratio in a centroid mode; and predicting an outcome of a therapeutic intervention associated with cancer based on changes in the protein turnover ratio, wherein non-essential amino acids of the-newly synthesized protein arc labeled with an isotope with low abundance through metabolism of the amino acids, an average enrichment of which is determined from the peptide containing such isotopically labeled amino acids and isotope labeling comprises introducing one of