Patent ID: 6287562
Filing Date: 2001-09-11
Classification: A61K,A61P,C07K,G01N

Abstract:
A method of inhibiting the growth of a cell bearing a Lewis.sup.Y antigen, said method comprising contacting said cell with a composition in an amount sufficient to inhibit the growth of said cell, said composition comprising:(a) a humanized Fv region of a light chain of an antibody and a humanized Fv region of a heavy chain of the same or of a different antibody, wherein said same or different antibody is a monoclonal antibody selected from the group consisting of B1 (ATCC accession number HB 10572), B3 (ATCC accession number HB 10573), and B5 (ATCC accession number HB 10569), provided that(i) if the light chain and the heavy chain are both from antibody B3, the light chain and the heavy chain are independently selected from the group consisting of: a light chain with a leucine residue in position 4 and a heavy chain with an arginine residue in position 82b,(ii) if the heavy chain and the light chain are from antibody B1, a serine is substituted at position 95 of the heavy chain, and(iii) if the heavy chain and the light chain are from antibody B5, a serine is substituted at position 95 of the heavy chain; and(b) an effector molecule selected from the group consisting of a drug, a cytotoxin, a radioisotope, and a liposome loaded with a chemotherapeutic agent or a toxin; wherein said Fv regions and said effector molecule are joined to form a single molecule that has the binding specificity of a monoclonal antibody selected from the group consisting of B1, B3, and B5, thereby inhibiting the growth of said cell.