Patent ID: 6700039
Filing Date: 2004-03-02
Classification: C12N

Abstract:
A method for the selective induction or suppression of sprouting in a vegetative storage organ of a plant comprising incorporating into the genome of said plant by transformation a DNA construct comprising a first polynucleotide sequence comprising at least one DNA sequence encoding an invertase or an inorganic pyrophosphatase operably linked to a tissue or organ selective promoter region and optionally to a transcription terminator region and a second polynucleotide sequence comprising at least one DNA sequence operably linked to and controlled by a controllable promoter region and optionally to a transcription terminator region whereby the DNA sequence(s) in said first polynucleotide sequence is expressed during dormancy of the vegetative organ derived from said transgenic plant resulting in effective suppression of sprouting and the said suppression is neutralised by inducing expression of the DNA sequence(s) in said second polynucleotide sequence from said controllable promoter region by external application of an inducing substance thus making restoration of sprouting of said vegetative storage organ dependent on the application of the inducer, so that sprouting is selectively induced or suppressed.