Patent ID: 6274324
Filing Date: 2001-08-14
Classification: C07K,C12N,G01N,Y10S

Abstract:
A specific binding reagent, comprising:(i) at least one variable domain antibody fragment which is essentially free from the rest of the originating antibody or antibodies;(ii) a peptide linking group, which does not contribute to the specific binding properties of the reagent, said peptide linking group being attached at or near the end of the variable domain antibody fragment and wherein said linking group comprises from 5 to 20 amino acid residues, is hydrophobic and includes at least one lysine residue and at least two amino residues selected from the group consisting of valine, leucine, iso-leucine, phenylalanine, tyrosine, tryptophan, proline and alanine and(iii) a solid surface or a tracer, coupled via the lysine residue of the linking group by covalent attachment to the variable domain antibody fragment.