Patent ID: 9169470
Filing Date: 2015-10-27
Classification: A61K,A61P,B82Y,C07K,C12N,C12Y,G01N

Abstract:
1. A dock and lock (DNL) complex comprising: a) a first fusion protein comprising (i) an anchoring domain (AD) from an A-kinase anchoring protein (AKAP), wherein the amino acid sequence of the AD moiety is selected from the group consisting of SEQ ID NO:2 and SEQ ID NO:5; and (ii) a first effector protein selected from the group consisting of an antibody and an antigen-binding fragment thereof; and b) a second fusion protein comprising (iii) a dimerization and docking domain (DDD) moiety, wherein the amino acid sequence of the DDD moiety is selected from the group consisting of residues 1-44 of human protein kinase A (PKA) RIIα, residues 1-44 of human PKA RIIβ and residues 12-61 of human PKA RIα; and (iv) a second effector protein that is a toxin, wherein the toxin is selected from the group consisting of ricin, abrin, ribonuclease, ranpirnase, rapLR1, DNase I, Staphylococcal enterotoxin-A, pokeweed antiviral protein, gelonin, diphtheria toxin, Pseudomonas exotoxin and Pseudomonas endotoxin; wherein two copies of the DDD moiety form a dimer that binds to the AD moiety to form the DNL complex.