Patent ID: 6613531
Filing Date: 2003-09-02
Classification: C12Q,G01N

Abstract:
A method to identify an agent which inhibits the binding of &sgr; to core RNA polymerase, a subunit thereof or a portion thereof, comprising:a) contacting the agent with core RNA polymerase, or an isolated &bgr;â€² subunit of RNA polymerase or a portion thereof, so as to form a complex; b) contacting the complex with &sgr; or a portion thereof; and c) detecting or determining whether the agent inhibits or prevents the binding of &sgr; to core RNA polymerase or to the isolated &bgr;â€² subunit of RNA polymerase or portion thereof, wherein the agent inhibits or prevents the interaction between &sgr; and one or more of residues 266, 275, 295 or 302 of &bgr;â€².