Patent ID: 6132993
Filing Date: 2000-10-17
Classification: C12P

Abstract:
A process for producing amphotericin B from Streptomyces nodosus while selectively inhibiting co-production of amphotericin A, comprising the steps of:a) cultivating Streptomyces nodosus in a nutrient medium suitable for the production of amphotericin B, said nutrient medium comprising a protein synthesis inhibitor selected from the group consisting of dihydrostreptomycin, neomycin, puromycin, kanamycin, gentamicin, tobramycin, amikacin, lincomycin, thiostrepton, chloramphenicol, spectinomycin, erythromycin and chlortetracycline, said protein synthesis inhibitor in a concentration of from about 0.1 .mu.g/ml to less than 1.0 .mu.g/ml, wherein said protein synthesis inhibitor selectively inhibits the co-production of amphotericin A; andb) recovering amphotericin B from the medium.