Patent ID: 8293496
Filing Date: 2012-10-23
Classification: C12Q,G01N

Abstract:
1. A method for determining the resistance of microbes to a specific antibiotic, comprising: (a) adding a quantity of the microbes to a nutrient medium that also contains a quantity of the antibiotic and adding to the nutrient medium reactive substances that can reactively modify microbes which are weakened by the antibiotic; (b) incubating the nutrient medium at a predetermined temperature for a predetermined time; (c) removing the microbes from the incubated nutrient medium; (d) applying the removed microbes and a matrix solution to a mass spectrometric sample support and drying the matrix solution on the sample support to produce a sample; (e) acquiring a mass spectrum of the sample; and (f) determining resistance of the microbes to the antibiotic by measuring modifications of the mass spectrum effected by the antibiotic compared to at least one reference mass spectrum of the microbes.