Patent ID: 9017987
Filing Date: 2015-04-28
Classification: C07K,C12N

Abstract:
1. A method of producing an influenza virus haemagglutinin 5(H5) polypeptide in a plant comprising the steps of: cloning an influenza virus H5 gene or nucleic acid encoding a functional equivalent thereof into a vector containing a targeting sequence to target a polypeptide expressed in the cytoplasm to components present in the plant, wherein the plant components are selected from the group consisting of endoplasmic reticuli and apoplasts; infiltrating at least a portion of the plant with the vector or transforming plant tissue with the vector so as to either transiently express the influenza virus H5 polypeptides in the cytoplasm and then import the influenza virus H5 polypeptides into the endoplasmic reticuli or apoplasts, and/or to create a transgenic plant wherein the influenza virus H5 polypeptides are expressed in the cytoplasm and then imported into the endoplasmic reticuli or apoplasts; and recovering the influenza virus H5 polypeptide expressed by the plant.