Patent ID: 7919298
Filing Date: 2011-04-05
Classification: C12N

Abstract:
1. An isolated polypeptide having lipase activity wherein said polypeptide is at least 80% identical to SEQ ID NO:2 and is a polypeptide having at least one of: a lipase activity (LU) relative to the absorbance at 280 nm (A280) of less than 500 LU/A280, in which one unit of LU (1 LU) is defined as the amount of enzyme capable of releasing 1 micro mol of butyric acid per minute at 30° C. at pH 7, and the absorbance of the polypeptide is measured at 280 nm; a Risk performance odor (R) below 0.1, in which R is calculated as the ratio between the amount of butyric acid released from a polypeptide washed swatch and the amount of butyric acid released from a reference polypeptide washed swatch, after both values have been corrected for the amount of butyric acid released from a non-polypeptide washed swatch; or a Benefit Risk factor (BR) of at least 1.8, in which BR is defined as the average wash performance (RP wherein said polypeptide comprises alterations of the amino acids at positions T231R+N233R+I255A+P256K and at least one of: S58A+V60S+A150G+L227G; or E210V/G; which positions correspond to SEQ ID NO:2.