Patent ID: 8105773
Filing Date: 2012-01-31
Classification: C12Q,Y10T

Abstract:
1. A method of preparing a standard gene transcript pattern characteristic of breast cancer or a stage thereof in an organism comprising at least the steps of: a) isolating mRNA from the cells of a sample of one or more organisms having the cancer or stage thereof, which isolated mRNA may optionally be reverse transcribed to cDNA; b) hybridizing the mRNA or cDNA of step (a) to a set of oligonucleotide probes specific for breast cancer or a stage thereof in an organism and sample thereof corresponding to the organism and sample thereof under investigation, wherein said set comprises at least 10 oligonucleotides, but contains less than 1000 oligonucleotides, and wherein at least 10 oligonucleotides of said set are selected from: c) assessing the amount of mRNA or cDNA hybridizing to each of said probes to produce a characteristic pattern reflecting the level of gene expression of genes to which said oligonucleotides bind, in the sample with breast cancer or a stage thereof, thereby obtaining a standard gene transcript pattern characteristic of breast cancer or a stage thereof in an organism.