Patent ID: 8685638
Filing Date: 2014-04-01
Classification: B01J,B01L,B82Y,C12M,G01N

Abstract:
1. A cell culture system, comprising: (a) a cell culture substrate; (b) a hydrogel layered on said cell culture substrate, wherein said hydrogel is non-adhesive for cells, wherein said hydrogel is dehydrated after being layered, and wherein at a plurality of discrete locations on hydrogel is deposited from about 1 to about 1000 nanoliters of one or a mixture of cell adherence materials, or a combination of one or a mixture of cell adherence materials and one or a mixture of soluble factors to form a microarray, wherein the cell adherence material is a biomolecule that promotes cellular adhesion, wherein said biomolecule is selected from the group consisting of proteins, peptides, oligonucleotides, polynucleotides, polysaccharide, proteoglycans, glycoproteins, glycosaminoglycans, and lipids; and (c) one or more cell types adhered to one or more locations of the microarray so as to form a micropattern of spatially separated cellular islands, wherein the micropattern is capable of being maintained for up to 7 days in cell culture.