Patent ID: 8185324
Filing Date: 2012-05-22
Classification: C12Q

Abstract:
1. A method of determining an initial concentration of a target nucleic acid in a sample using real-time nucleic acid amplification data, the method comprising: (a) amplifying the target nucleic acid and detecting signals from an amplified product at different points of time during the amplification; (b) obtaining an amplification efficiency of the target nucleic acid with respect to time using the detected signals, wherein the amplification efficiency is represented by Formula 1 below, wherein E (c) formulating an amplification efficiency function with respect to amplification time, wherein the amplification efficiency function is composed of geometric mean values of the amplification efficiency with respect to time; (d) applying the amplification efficiency function to calculate a point of amplification time at which the amplification efficiency has a maximum value; and (e) determining the initial concentration of the target nucleic acid in the sample using the calculated point of amplification time, wherein the initial concentration is determined using a reference curve, said reference curve being obtained employing the target nucleic acid of various known initial concentrations.