Patent ID: 8426575
Filing Date: 2013-04-23
Classification: A61K,C07H,C12N,G01N

Abstract:
1. An isolated polynucleotide molecule comprising a DNA sequence encoding an infectious RNA molecule that encodes a genetically modified classical swine fever virus (CSFV) mutant of the highly pathogenic native Brescia strain, wherein said CSFV mutant encodes a genetically modified E1 glycoprotein set forth in SEQ ID NO:3, wherein mutated non-glycosylated amino acid alanine in the sequence AVTS (SEQ ID NO:9) has replaced glycosylated asparagine in the sequence NVTS (SEQ ID NO:8) at position 500 and mutated non-glycosylated amino acid alanine in the sequence ACTP (SEQ ID NO:11) has replaced glycosylated asparagine in the sequence NCTP (SEQ ID NO:10) at position 513, said mutated amino acids having a glycosylation pattern different from the amino acids present in positions 500 and 513 of said highly pathogenic native Brescia strain, wherein said genetically modified CSFV mutant is attenuated and unable to produce a pathogenic CSFV infection in a porcine animal.