Patent ID: 6015716
Filing Date: 2000-01-18
Classification: G01N,Y10S,Y10T

Abstract:
A method of detecting endotoxin in lipid complexes, lipid bilayers or liposomes, the method comprising the steps of:(a) preparing an aqueous suspension of micelles from an aqueous suspension of lipid bilayers, liposomes or lipid complexes comprising a lipid by completely solubilizing the lipid bilayer, liposome or lipid complex suspension with a detergent so as to form micelles comprising the lipid and the detergent;(b) assaying the micellar suspension for the presence of endotoxin, wherein, the detergent does not inhibit the endotoxin assay by a factor of more than about 100 and wherein the endotoxin assay is effective to detect endotoxin in the micellar suspension at an endotoxin concentration as low as about 0.1 EU/ml.