Patent ID: 8263324
Filing Date: 2012-09-11
Classification: C07B,C07H,C12N

Abstract:
1. A method of isolating nucleic acid from a blood sample, said method comprising: (a) selectively isolating leucocytes from said sample by binding said leucocytes to a first solid support by means of at least two different binding partners specific for leucocytes; (b) lysing said isolated leucocytes; (c) binding nucleic released from said lysed leucocytes to said first solid support; and (d) contacting the nucleic acid of step (c) with an additional amount of a second solid support, and binding the nucleic acid to said second solid support; wherein the method is performed using an automated system for handling the first solid support and second solid support during cell lysis, nucleic acid binding or both, and wherein the first solid support and second solid support are transferred from a first tube to a second tube by the automated system, wherein the at least two different binding partners recognize or are capable of binding specifically to CD45 and CD15, and wherein the first solid support comprises polymeric particles.