Patent ID: 6218116
Filing Date: 2001-04-17
Classification: C12Q,G01N

Abstract:
A method for simultaneously hybridizing in a liquid medium a plurality of different nucleic ligands each in the free state in said liquid medium, said ligands belonging to a same component or several components in the free state in said liquid medium, said method comprising:a) providing a same support for plural hybridization on which there are distributed a plurality of immobilizing sites, separated from each other;b) temperature conditioning said support so as to have different local temperature values respectively in said immobilizing sites, at least along one reference direction of said support;c) providing a plurality of different nucleic anti-ligands capable of specifically hybridizing respectively with nucleic ligands;d) determining for each of said anti-ligands the optimum temperature value at which the corresponding ligand/anti-ligand complex is stable;e) distributing and immobilizing said anti-ligands in said different immobilizing sites, so as to substantially match said local temperature values with said optimum temperature values, as an exogenous parameter from which the stability of said ligand/anti-ligand complexes depend directly or indirectly;f) contacting said liquid medium with said support, with the plurality of said anti-ligands immobilized in said support; andg) sensing the presence and/or the quantity of the different complexes formed between said immobilized anti-ligands and corresponding ligands of the liquid medium.