Patent ID: 6087088
Filing Date: 2000-07-11
Classification: G01N

Abstract:
A binding assay for determining an amount of an analyte in a sample containing interfering factors comprisinga. contacting said sample containing said analyte with(1) a labeled specific binder, labeled with a first label, said labeled specific binder binding to said analyte to form an analyte-labeled specific binder complex,(2) a solid phase containing an immobilized analyte derivative or mimic, said analyte derivative or mimic binding to said labeled specific binder, and(3) a labeled reference binder, labeled with a second label, which binds to said analyte derivative or mimic and said interfering factors but which does not bind to said analyte,such that said first label is different from said second label,b. separating said first and second labels which are bound to said solid phase from said unbound labels,c. determining the amount of said first and second labels which are bound to said solid phase,d. comparing the amount of said bound first label with that of reference standards to determine the amount of analyte present , ande. correcting the amount of said analyte present by (1) comparing said amount of said bound second label with that of reference standards to calculate a correction factor, and (2) applying said correction factor to the amount of analyte determined in step d to correct the amount of said analyte.