Patent ID: 8021842
Filing Date: 2011-09-20
Classification: C12Q

Abstract:
1. A method of screening for the presence or absence of a nucleotide allele of a polymorphism in multiple DNA samples, the method comprising the steps of: combining the following under hybridization conditions: thereby forming RNA/DNA heteroduplexes; removing any mismatched nucleotide at the polymorphic site from the RNA probes in the heteroduplexes; contacting the heteroduplexes with a nucleotide triphosphate complementary to the second nucleotide allele under nucleic acid polymerization conditions; isolating heteroduplexes that have incorporated the nucleotide triphosphate complementary to the second nucleotide allele; and determining the unique tags of the single stranded DNA fragments of the isolated heteroduplexes to identify genomic DNA samples having the second nucleotide allele at the polymorphic site in the sequence of interest, thereby screening the multiple DNA samples for the presence or absence of the second nucleotide allele.