Patent ID: 6153396
Filing Date: 2000-11-28
Classification: A61K,A61P,C07H,G01N,Y02A,Y10S

Abstract:
A method for identifying a substance that interacts with a periplasmic molecular chaperone, thereby preventing, inhibiting or enhancing the binding of said periplasmic molecular chaperone and a pilus subunit, the method comprisingadding the substance to be tested to a system comprising the periplasmic molecular chaperone in an immobilized form and the pilus subunit in a solubilized form and determining the change in binding between the pilus subunit and the periplasmic molecular chaperone caused by the addition of the substance, oradding the substance to be tested to a system comprising the pilus subunit in an immobilized form and the periplasmic molecular chaperone in a solubilized form and determining the change in binding between the pilus subunit and the periplasmic molecular chaperone caused by the addition of the substance, oradding the substance to be tested to a system comprising the pilus subunit as well as the periplasmic molecular chaperone in a solubilized form and determining the change in binding between the pilus subunit and the periplasmic molecular chaperone caused by the addition of the substance, oradding the substance to be tested to a system comprising the pilus subunit as well as the periplasmic molecular chaperone in a solubilized form and measuring the change in binding energy caused by the addition of the substance, and detecting any change in the binding energy between the pilus subunit and the periplasmic molecular chaperone.