Patent ID: 8912128
Filing Date: 2014-12-16
Classification: G01N

Abstract:
1. A method for detecting Vibrio cholerae by detecting cholera toxin consisting of one A subunit and five B subunits, the method comprising: applying a sample containing cholera toxin onto a carbohydrate chip, wherein the carbohydrate chip comprises (i) a solid substrate and (ii) GM1 pentasaccharide, GM2 tetrasaccharide, asialo GM1 tetrasaccharide, GM3 trisaccharide, galactose-β 1,3-N-acetylgalactosamine, lactose, and sialic acid immobilized on a surface of the solid substrate, wherein the carbohydrate chip has a visual limit of detection (LOD) of 5 ng/mL for cholera toxin produced by detecting the cholera toxin by fluorescence image analysis.