Patent ID: 8906686
Filing Date: 2014-12-09
Classification: A61F,A61K,A61L,C12N

Abstract:
1. A method for preparation of an implantable construct for implantation into a cartilage lesion, said method comprising steps: providing a support matrix comprising a plurality of pores; suspending chondrocytes in a collagen solution; seeding said suspended chondrocytes into the pores of said support matrix, wherein said suspended chondrocytes are seeded at a density of about 3 to about 60 million cells/mL of the collagen solution; and culturing said chondrocytes seeded in the support matrix under conditions sufficient to activate and promote proliferation of the chondrocytes within said support matrix, wherein said conditions comprise: applying a cyclic hydrostatic pressure from about 0.5 MPa to about 3 MPa above atmospheric pressure, at a frequency about 0.1 Hz to about 2.0 Hz, for about 6 days to about 14 days followed with a resting period at atmospheric pressure for about 7 to about 28 days; and perfusing said seeded support matrix with a culture medium at a rate of perfusion from about 5 μL/min to about 50 μL/min.