Patent ID: 9029162
Filing Date: 2015-05-12
Classification: G01N,Y10T

Abstract:
1. A method for determining the presence or amount of testosterone in a biological sample, the method comprising: (a) providing a biological sample containing testosterone; (b) partially purifying the biological sample using supported liquid extraction, thereby providing a partially purified sample comprising testosterone, wherein the supported liquid extraction includes using diatomaceous earth and an organic water-immiscible solvent system, and wherein the organic water-immiscible solvent system comprises a solvent selected from the group consisting of hexane, octanol, chloroform, dichloromethane, diethyl ether, cyclohexane, pentane, N-heptanes, benzene, n-butyl chloride, butanol, methylene chloride, and mixtures thereof; (c) chromatographically separating testosterone from the other components in the partially purified sample using liquid chromatography, wherein the liquid chromatography comprises using at least one guard column; and (d) analyzing the chromatographically separated testosterone by mass spectrometry to determine the presence or amount of testosterone in the biological sample; wherein the step of partially purifying the biological sample using supported liquid extraction comprises eluting with the organic water-immiscible solvent system under slight vacuum and under gravity.