Patent ID: 9080210
Filing Date: 2015-07-14
Classification: C12P,C12Q

Abstract:
1. A method for identifying the sample origin of ligated and/or amplified sample DNA comprising: a) obtaining a plurality of first adapters or PCR amplification primers comprising first nucleotide sequence identifiers and second adapters or PCR amplification primers comprising second nucleotide sequence identifiers; b) performing ligation and/or PCR amplification reactions on sample DNAs of different origins using different combinations of the first and second adapters and/or PCR amplification primers, to provide ligated and/or amplified prepared sample DNAs each comprising the first and second nucleotide sequence identifiers in addition to the sequence captured and/or amplified from the sample DNA, wherein a unique combination of first and second nucleotide sequence identifiers is used for each sample DNA of each different origin; c) determining at least the sequence of the of first and second nucleotide sequence identifiers using high throughput sequencing; d) determining the sample origin of the ligated and/or amplified prepared sample DNAs based on the unique combination of first and second nucleotide sequence identifiers.