Patent ID: 6939689
Filing Date: 2005-09-06
Classification: A61K,C07K,C12N,C12Q

Abstract:
1. A method for generating a mutagenized polynucleotide comprising: a) annealing a poly-binding nucleic acid strand to two or more mono-binding nucleic acid strands to generate an annealed heteromeric complex of nucleic acid strands, wherein said annealed complex comprises about 10, 100, 1,000, 10,000, 100,000 or 1,000,000 bases; b) subjecting unhybridized single-stranded ends of the annealed mono-binding nucleic acid strands in the heteromeric complex to an exonuclease treatment that degrades said unhybridized ends; and c) subjecting the annealed hertomeric complex to polymerase-based extension.