Patent ID: 6395503
Filing Date: 2002-05-28
Classification: C09K,C12Q,G01N,Y10S

Abstract:
A chemiluminescent reagent composition characterized by the chemiluminescence produced in the presence of a peroxide, intensity of which varies depending on concentration of peroxidase enzyme, and comprising, as the major ingredients, a charge-transferring complex of N,Nâ€²-disubstituted-9,9â€²-bisacridinium salt, expressed by a broad absorption band having a maximum at around 550 nm in the ultraviolet absorption spectrum and shown by the general formula (1): (wherein, R1 and R2 are each selected from the group consisting of an alkyl, aryl and halogenated aryl groups, and may be the same or different; R3, R4, R5 and R6 are each selected from the group consisting of hydrogen, alkyl, aryl, alkoxy and aryloxy groups and halogen, and may be the same or different; and XÂ· is an acid radical as the residue left by the electron transferring from the counter anion of the bisacridinium salt as the precursor), and an N,N-disubstituted carboxylic amide compound shown by the general formula (2): (wherein, R1 is selected from the group consisting of hydrogen, alkyl group having a carbon number of 1 to 10, an alkenyl group having a carbon number of 2 to 10 and an aryl group having a carbon number of 6 to 20, wherein the aryl group may be substituted with an alkyl, nitro, hydroxyl or amino group, halogen or the like; R2 is selected from the group consisting of methyl and ethyl groups; and R3 is selected from the group consisting of an alkyl group having a carbon number of 1 to 10, an alkenyl group having a carbon number of 2 to 10 and an aryl group having a carbon number of 6 to 20, wherein the aryl group may be substituted with an alkyl, nitro, hydroxyl or amino groups, halogen, and R1 and R3 may be bonded to each other to form a ring together with the carbon atom and nitrogen atom which are in the amide group, respectively, to which each of R1 and R3 are bonded).