Patent ID: 7544487
Filing Date: 2009-06-09
Classification: A61K,A61P,C07K,C12N

Abstract:
1. A method of preparing an immunotoxin comprising culturing a mammalian host cell, wherein said host cell is transfected with (a) a first nucleic acid sequence encoding a fusion polypeptide, wherein said fusion polypeptide comprises a non-mammalian ribonuclease from (b) a second nucleic acid sequence encoding a second polypeptide comprising a second immunoglobulin variable domain, wherein said first and second immunoglobulin variable domains together form an antigen binding site; wherein said non-mammalian ribonuclease is fused to the N-terminus of said first immunoglobulin variable domain, wherein said non-mammalian ribonuclease bears an N-terminal pyroglutamate residue, and wherein the first immunoglobulin variable domain is a light chain variable domain.