Patent ID: 8454974
Filing Date: 2013-06-04
Classification: A61K,A61P,C12N

Abstract:
1. An isolated nucleic acid molecule which encodes human hepatitis C virus of genotype 4a/JFH1, wherein said molecule: (i) is capable of expressing said virus when transfected into cells, (ii) is capable of infectivity in vivo, and (iii) encodes an amino acid sequence with at least 95% sequence identity to that of SEQ ID NO 3, which (iv) comprises an intergenotypic junction in the cytoplasmic part of NS2 and at least one adaptive mutation in the amino acid sequence of E1, NS2 or NS5A, selected from the group consisting of T827A, E989K, T1989I and V2436L, wherein said mutation is numbered according to the HCV sequence of SEQ ID NO:3.