Patent ID: 6537795
Filing Date: 2003-03-25
Classification: C12N,C12P,C12R,Y10S

Abstract:
A method for selecting streptococcus strains capable of producing hyaluronic acid with molecular weight exceeding six million, comprising the steps of:a. subjecting wild type streptococci strains to mutagenesis; b. thereafter cultivating the strains of streptococci individually in culture medium in a reactor, wherein the culture medium is free of metal ions which promote degradation of hyaluronic acid and does not release from the reactor metal ions which promote the degradation of hyaluronic acid; and c. selecting a supercapsulated mutated strain of a streptococcus from the cultivated strains, the supercapsulated strain having a mucoid morphology and supercapsulated members thereof being capable of forming capsules having a diameter of greater than 4 &mgr;m.