Patent ID: 8834953
Filing Date: 2014-09-16
Classification: A62D,C12N

Abstract:
1. A method for preparing a magnetic mesoporous silica whose surface strongly binds a histidine-tagged protein, the method comprising the steps of: preparing a mesoporous silica, adding the mesoporous silica to an aqueous solution of a precursor, and stirring the resulting mesoporous silica-containing solution to load the precursor onto the mesoporous silica, thus obtaining a precursor-loaded mesoporous silica; filtering the precursor-loaded mesoporous silica, freezing the filtered precursor-loaded mesoporous silica at a temperature between −70° C. and -80° C., and then vacuum-drying the frozen precursor-loaded mesoporous silica at room temperature; reducing the vacuum-dried precursor-loaded mesoporous silica in a hydrogen atmosphere at a temperature of 400˜800° C. for 2-3 hours, thus obtaining a magnetic mesoporous silica having a plurality of pores formed therein; adding the magnetic mesoporous silica to a transition metal aqueous solution containing a transition metal or an ion thereof, ultrasonically treating the resulting magnetic mesoporous silica-containing solution, and stirring the ultrasonically treated magnetic mesoporous silica containing solution; filtering the stirred magnetic mesoporous silica containing solution thereby obtaining a filtered material, freezing the filtered material at a temperature between −70° C. and −80° C., and then vacuum-drying the frozen material at room temperature; and reducing the vacuum-dried material in a hydrogen atmosphere at a temperature of 400˜800° C. for 2-3 hours to adsorb and coat the transition metal or an ion thereof onto the pores of the magnetic mesoporous silica.