Patent ID: 6458533
Filing Date: 2002-10-01
Classification: B01J,C12Q,C40B

Abstract:
A method of determining which of a plurality of ESTs are complementary to a given nucleic acid, comprising,a) incubating an immobilized array of oligonucleotide probes, wherein at least one of said probes is complementary to a portion of a first strand of each of said plurality of ESTs, and wherein the second strands of at least two of said ESTs are complementary to different or overlapping portions of said given nucleic acid, with a test sample which may contain said given nucleic acid, to obtain, if said test sample contains said given nucleic acid, hybridization products between said oligonucleotide probes and said given nucleic acid, b) incubating the hybridization products in said array with a detector oligonucleotide, wherein, for each hybridization product, said detector oligonucleotide is complementary to a portion of a first strand of an EST to which the oligonucleotide probe in said hybridization product is complementary, but which is complementary to a different portion of that EST strand than is said oligonucleotide probe, and c) detecting which oligonucleotide probes of said array are labeled by said detector oligonucleotide, thereby determining which of said plurality of ESTs are complementary to said given nucleic acid, wherein said array of oligonucleotide probes is immobilized on a region of a combination, wherein said combination comprises i) a surface comprising a number of spatially discrete, substantially identical, regions equal to the number of ESTs to be studied, each region comprising ii) a number of different anchors equal to the number of ESTs to be studied, each anchor in association with iii) a bifunctional linker which has a first portion that is specific for the anchor, and a second portion that comprises an oligonucleotide probe which is specific for at least one of said ESTs.