Patent ID: 6673631
Filing Date: 2004-01-06
Classification: C12N,C12Q,Y10S

Abstract:
A method for isolating a defined quantity of a DNA target material from other material in a medium by:a. providing a medium including the DNA target material; b. providing a discrete quantity of porous silica magnetic particles capable of reversibly binding a definable quantity of the DNA target material, the particles having an average particle diameter of about 5.0 to 8.5 microns, a nitrogen BET surface area of about 18 to 55 microns gm and an acid leach resistance of less than about 7 ppm Fe2O3, the amount of DNA target material provided in step (a) being in excess of the binding capacity of the particles; c. forming a complex of the silica magnetic particles and the DNA target material by combining the silica magnetic particles and the medium; d. removing the complex with the DNA target material from the medium by application of an external magnetic field; and e. separating the DNA target material of step (c) from the complex by eluting the DNA target material, whereby a defined quantity of the DNA target material is obtained.