Patent ID: 6830886
Filing Date: 2004-12-14
Classification: B01J,C07B,C07H,C12Q,C40B

Abstract:
A support comprising polynucleotides covalently linked at their 5â€²- or 3â€²-termini to at least one major surface of said support through at least one bifunctional spacer and at least one bifunctional linker, wherein:said polynucleotides have a length of from 200 to 600 bp; said bifunctional linker is selected from the group of rigid homobifunctional linkers consisting of 1,4-disubstituted benzene, 2,7-substituted fluorene, 2,6-substituted naphthalene, 2,6-substituted anthracene, 2,7-substituted phenanthrene, 4,4â€²-substituted biphenyl, 4,4â€²-substituted benzoin (C6H5â€”COâ€”CHâ€”(OH)â€”C6H5), 4,4â€²-substituted benzil (C6H5â€”COâ€”COâ€”C6H5), 4,4â€²-substituted benzophenone (C6H5), 4,4â€²-substituted diphenylmethane (C6H5â€”CH2â€”C6H5), 4,4â€²-substituted stilbene (C6H5â€”CH&boxH;CHâ€”C6H5), and 1,3-substituted allene (CH2&boxH;C&boxH;CH2); said polynucleotides are covalently bound to a functional group of said bifunctional linker through a primary amino group attached, on the 3-â€² or 5â€²-terminus through an alkane having a length of from 6 to 18 methylene groups or though a polyether having from 2 to 20 repeating units; and the polynucleotides are prepared by amplification.