Patent ID: 9217171
Filing Date: 2015-12-22
Classification: C12Q,G01N

Abstract:
1. A colorimetric device for detecting, in an aqueous solution of interest, a hydrolytic enzymatic activity capable of hydrolyzing at least one biopolymer of interest, which device comprises (i) a substrate delimited by at least one upper surface, and (ii) a transparent detection layer, having a first thickness and including said biopolymer, wherein said transparent detection layer includes an upper surface, for receiving said aqueous solution of interest and forming a first interface between the air and said detection layer, and a lower surface for forming a second interface between said detection layer and said substrate, wherein said first interface and said second interface are able to generate an optical interference, wherein said detection layer is adapted so that after application of said aqueous solution of interest, in the absence of said hydrolytic enzymatic activity, said detection layer preserves said first thickness, and after said application of said aqueous solution of interest, when said detection layer includes said hydrolytic enzymatic activity, said detection layer has a second thickness which is thinner than said first thickness, wherein said first thickness and/or said second thickness of said detection layer are adapted to generate a colour by an optical interference caused by the recombining of the light beams reflected at said first interface and at said second interface, and wherein said upper surface of said transparent detection layer is not covered by an additional nanoparticle layer.