Patent ID: 6090615
Filing Date: 2000-07-18
Classification: C12N

Abstract:
A method for obtaining an extract from a mycelium-containing culture medium, comprising the steps of:inoculating basidiomycetes in a solid culture medium containing bagasse as a substrate;proliferating mycelium of the basidiomycetes;applying pressure to the solid culture medium containing proliferated mycelium to obtain a squeeze liquid therefrom;separating a mycelium-containing solid residue from the squeeze liquid;adding water and an enzyme containing .beta.-1,3-glucanase as a main ingredient together with at least one enzyme selected from the group consisting of protease, chitinase and cellulase to the mycelium-containing solid residue to produce a mixture;stirring the mixture while maintaining the mixture at 30 to 60.degree. C. to lyse mycelial cell walls; andheating the resulting cell wall lysis product-containing liquid up to a temperature of 95.degree. C. to inactivate the enzymes and perform sterilization, thereby producing an extract of the mycelium-containing culture medium.