Patent ID: 9085793
Filing Date: 2015-07-21
Classification: C12Q,G01N

Abstract:
1. A method of analyzing circulating drug metabolites with drug interaction potential, said method comprising: providing a clinical plasma sample from a subject, wherein said clinical plasma sample comprises a drug at a first concentration and a plurality of metabolites of said drug; providing a standard control plasma sample comprising said drug at a standard concentration, said standard control plasma sample being essentially free of metabolites of said drug, wherein said standard concentration is substantially the same as, or brackets said first concentration; adding said clinical plasma sample to a first in vitro test system; adding said standard control plasma sample to a second in vitro test system; assaying said clinical plasma sample and said standard control plasma sample for activity or expression of drug-metabolizing enzymes and/or drug transporters in said first and second in vitro test systems; and analyzing changes in activity or expression of drug-metabolizing enzymes and/or drug transporters in said first and second in vitro test systems to determine circulating drug metabolites with drug interaction potential based upon inhibitory and/or inducing effects on said drug-metabolizing enzymes and/or drug transporters.