Patent ID: 6670191
Filing Date: 2003-12-30
Classification: G01N,Y10T

Abstract:
A method for quantitatively analyzing fragmented red blood cells, comprising the steps of:adding a nucleic acid staining fluorochrome dye to a blood sample for preparation of a specimen containing particles; allowing the specimen to flow through a flow cytometer, and measuring a scattered light intensity and a fluorescent light intensity for each particle in the specimen; preparing a two-dimensional particle distribution diagram on the basis of the measured scattered light intensity and fluorescent light intensity; establishing a red blood cell distribution area on the two-dimensional distribution diagram, and counting particles falling within the red blood cell distribution area for determination of the total number A of red blood cells; establishing a fragmented red blood cell distribution area in the red blood cell distribution area, and counting particles falling within the fragmented red blood cell distribution area for determination of the number B of fragmented red blood cells; and calculating a value B/A, and calculating a content by percentage F of the fragmented red blood cells from a conversion function F=f (B/A).