Patent ID: 6514741
Filing Date: 2003-02-04
Classification: C12N

Abstract:
A fusion protein produced by a method comprising:culturing a host cell into which has been introduced a vector comprising the following operably linked elements: (a) a transcriptional promoter; (b) a DNA construct encoding a fusion protein, wherein the DNA construct encodes a polypeptide that is selected from the group consisting of: (i) the amino acid sequence of SEQ ID NO: 2 from residue number 1 (Met), to residue number 23 (Ser); (ii) the amino acid sequence of SEQ ID NO: 15 from residue number 1 (Met), to residue number 18 (Ala); (iii) the amino acid sequence of SEQ ID NO: 2 from residue number 24 (Leu), to residue number 43 (Lys); (iv) the amino acid sequence of SEQ ID NO: 15 from residue number 19 (Arg), to residue number 42 (Arg); (v) the amino acid sequence of SEQ ID NO: 2 from residue number 24 (Leu), to residue number 276 (Ile); (vi) the amino acid sequence of SEQ ID NO: 15 from residue number 19 (Arg), to residue number 275 (Arg); (vii) the amino acid sequence of SEQ ID NO: 2 from residue number 24 (Leu), to residue number 314 (Leu); (viii) the amino acid sequence of SEQ ID NO:15 from residue number 19 (Arg), to residue number 312 (Leu); (ix) the amino acid sequence of SEQ ID NO: 2 from residue number 44 (Val), to residue number 276 (Ile); (x) the amino acid sequence of SEQ ID NO: 15 from residue number 43 (Val), to residue number 275 (Arg); (xi) the amino acid sequence of SEQ ID NO: 2 from residue number 44 (Val), to residue number 314 (Leu); (xii) the amino acid sequence of SEQ ID NO: 15 from residue number 43 (Val), to residue number 312 (Leu); (xiii) the amino acid sequence of SEQ ID NO: 2 from residue number 277 (Lys), to residue number 314 (Leu); (xiv) the amino acid sequence of SEQ ID NO:15 from residue number 276 (Thr), to residue number 312 (Leu); and (xv) the amino acid sequence of SEQ ID NO:24; and at least one other DNA segment encoding an additional polypeptide, wherein the first and other DNA segments are connected in-frame; and encode the fusion protein; and (c) a transcriptional terminator; and recovering the protein encoded by the DNA segment.