Patent ID: 6800608
Filing Date: 2004-10-05
Classification: A61K,C07K,G01N

Abstract:
A stabilized vancomycin conjugate prepared by a method comprising:(a) forming a vancomycin conjugate comprising a vancomycin member bound to a ligand, said ligand being bound to a ligand-binding partner immobilized on a solid support, wherein said conjugate is formed under conditions that minimize intermolecular hydrogen bonding between vancomycin members; (b) mixing said conjugate with at least one stabilizing agent that prevents dimerization of said vancomycin member, wherein the pH of the mixture is between about pH 7 to pH 9; (c) heating the mixture at a temperature between about 40Â° and 50Â° C. for about 3 to 14 days to ensure colloidal stability; and (d) storing the heat-treated mixture obtained in step (c) in a diluent buffer having a pH of about 6.5 to 8.5.