Patent ID: 8835134
Filing Date: 2014-09-16
Classification: A61K,A61P,C07J,C12P

Abstract:
1. A method for preparing cycloastragenol-6-O-β-D-glucoside, characterized by comprising the following steps: a. using astragaloside IV or Astragali extracts prepared by a conventional method as raw materials and adding an appropriate solvent thereinto to form a raw material solution; when the raw material is astragaloside IV, the concentration of astragaloside IV in the solution is 0.01-0.1% W/V; and when the raw material is Astragali extracts, the ratio of extracts:solution is 1:15-1:1000 W:V; b. adding hydrolase and allowing for hydrolysis at a constant temperature to obtain a hydrolysate, wherein said hydrolysis is conducted for 12-72 hours at a constant temperature from 40 to 55° C. and a suitable pH of the solution is from 4 to 7, said hydrolase being selected from the group consisting of β-glycosidase, β-glucosidase, and a mixture of one of these enzymes and one or more enzymes selected from the group consisting of cellulase, glucanase, xylanase, glucoamylase, pectinase and amylase; and when the substrate is astragaloside IV, the ratio of the substrate to the enzymes is 1:1-50 W:W; and when the substrate is Astragali extracts, the ratio of the enzymes to the substrate is 1:100-10:1 W:W; c. separating the hydrolysate with macroporous adsorption resin; wherein when the raw material is Astragali extracts, the ratio of raw material:resin is 1:20-4:1 g:ml; and when the raw material is astragaloside IV, the ratio of raw material:resin is 0.1:1-20:1 mg:ml, wherein said separation is conducted by a process comprising d. purifying the separated product.