Patent ID: 9181534
Filing Date: 2015-11-10
Classification: C12N,C12P

Abstract:
1. An enzyme mixture comprising a first enzyme and a second enzyme, wherein said first enzyme is a DNA polymerase or reverse transcriptase, and said second enzyme is the wild type Pfu DNA polymerase comprising the amino acid sequence of SEQ ID NO. 12, except that it is mutated at an amino acid position selected from the group consisting of: Y410, D543, K593, G387, G388, and the following amino acid substitutions: Y385N, Y385W, Y385L, Y385H, Y385Q, and Y385S, and wherein said second enzyme possesses reduced 3′-5′ exonuclease activity and reduced 5′-3′ DNA polymerization activity as compared to the wild type Pfu DNA polymerase.