Patent ID: 6576428
Filing Date: 2003-06-10
Classification: C12N,G01N

Abstract:
A method to label antigen-specific T cells with a product secreted and released by the cells, wherein the product is secreted in response to antigen stimulation of said T cells, which method comprises:a) exposing the antigen-specific T cells to at least one antigen specifically recognized by a T cell receptor under conditions effective to elicit antigen-specific stimulation of at least one antigen-specific T cell; b) modifying the surface of the antigen-specific T cells to contain a capture moiety specific for the product; and c) culturing the cells, wherein the cells are cultured under conditions wherein the product is secreted, released and specifically bound to the capture moiety, thereby labeling the product-secreting cells, and wherein steps a), b) and c) can be performed in any order or simultaneously.