Patent ID: 8017323
Filing Date: 2011-09-13
Classification: C07H

Abstract:
1. A method of synthesizing a reaction product by nucleic acid-templated synthesis, the method comprising the steps of: (a) providing a mixture comprising a plurality of different first reactive units each attached to corresponding different first oligonucleotides comprising a codon sequence, wherein each oligonucleotide sequence is indicative of the first reactive unit attached thereto; (b) providing a second reactive unit attached to a second oligonucleotide comprising an anti-codon sequence complementary to the codon sequence of at least one first oligonucleotide attached to a first reactive unit; (c) annealing the codon sequence of at least one of the first oligonucleotides with the anti-codon sequence of the second oligonucleotide to induce a reaction between the first and second reactive units to form a first reaction intermediate covalently attached at least to a first oligonucleotide; and (d) after step (c), combining the first reaction intermediate, co-existing with at least one of the reactive units, with a free reactant selectively reactive with the first reaction intermediate thereby synthesizing a first reaction product attached to the first oligonucleotide, wherein the free reactant (i) is not present during any of steps (a), (b) or (c); and (ii) is more reactive with the first reaction intermediate than with at least one of the reactive units in the mixture.