Patent ID: 8921120
Filing Date: 2014-12-30
Classification: C07K,G01N

Abstract:
1. A method of determining the presence of multimers of a macromolecule monomer in a sample containing the macromolecule, comprising the steps of: determining the total concentration of the macromolecule in the sample; determining the active concentration of the macromolecule in the sample by putting the sample in contact with a sensor surface of a biosensor system arranged to perform a biosensor-based detection method, wherein physical characteristics of the macromolecule monomer are used to determine the active concentration; providing a reference value for the active concentration of a monomer-standard reference-sample with a total concentration of the macromolecule corresponding to the total concentration of the macromolecule determined for the sample comparing the determined active concentration of the macromolecule in the sample with the reference value for the active concentration of the monomer reference-sample; and wherein a lower active concentration of the macromolecule in the sample compared to the reference value for the active concentration of the monomer reference-sample provides a measure of the presence and level of multimers in the sample.