Patent ID: 6551589
Filing Date: 2003-04-22
Classification: A61K,C12N

Abstract:
A method of reducing the chance of graft versus host disease in a patient host treated for a malignancy and receiving a bone marrow transplant from a donor and reducing the chance of infection and relapse of the malignancy, comprising the steps of:obtaining for cellular selection mononuclear cells containing T-cells from the donor; obtaining, from the host, cell derived antigens, including malignancy antigens, recognized by T-cells included in the donor mononuclear cells; incubating the mononuclear cells from the donor with host antigens, including malignancy antigens, to thereby activate donor mononuclear cells that are specific to the malignancy antigens; obtaining, from the host, cell derived normal antigens recognized by T-cells included in the donor mononuclear cells but not including malignancy antigens; incubating activated mononuclear cells from the donor with host antigens not including malignancy antigens and recovering the donor mononuclear cells that do not attach to the host antigens; infusing the recovered cells into the host; and in coordination with infusing the recovered cells into the host, infusing into the host inoculum from the donor which constitutes the bone marrow transplant after substantial depletion of T-cells from the inoculum.