Patent ID: 6608238
Filing Date: 2003-08-19
Classification: A01K,A61K,C12N

Abstract:
A method of producing a trans-somatic mammal, wherein said method provides the incorporation of a DNA sequence into the secretory cells of the mammary gland to alter the composition of the milk, wherein said method comprising the steps of:a) providing a retroviral vector containing a DNA sequence encoding a polypeptide; b) packaging said retroviral vector in a cell line to produce retroviral particles; c) preparing a solution comprising the retroviral particles and cell line producing said retroviral particles; and d) delivering said solution into the mammary gland to allow the incorporation of the DNA into the secretory cells of the mammary gland, wherein a substance, with a density higher than the density of the solution comprising the retroviral particles and cell line producing said retroviral particles, is delivered into the mammary gland after the delivery of the solution comprising the retroviral particles and cell line producing said retroviral particles, wherein said substance displaces the solution upwards in the mammary gland.