Patent ID: 7572595
Filing Date: 2009-08-11
Classification: G01N

Abstract:
1. A method for examining the distribution of myelin in tissue samples using gonadotropin-releasing hormone receptor (GnRH-R) as a biomarker comprising the following steps: (i) preparing myelin rich tissue samples, wherein the samples are selected from the group consisting of corpus callosum, cingulum, fimbria of hippocampus, internal capsule, stria medullaris of thalamus and optic tract; (ii) reacting the samples with an anti-GnRH-R antibody and washing the samples; (iii) reacting the washed samples of step (ii) with a color coupler linked secondary antibody, washing the samples and placing on a slide; (iv) inducing color development by adding a substrate reactive to the color coupler to the samples of step (iii); and (v) observing the prepared slides under a microscope for positive staining of GnRH-R wherein positive staining for GnRH-R indicates the distribution of myelin in tissues samples.