Patent ID: 9080204
Filing Date: 2015-07-14
Classification: C12Q

Abstract:
1. A method for detecting the presence of a specific nucleic acid in a biological sample that has been maintained for a period of time without refrigeration, comprising: adding the biological sample to a composition thereby forming an aqueous mixture, wherein the composition contains: one or more chaotropes; one or more detergents; one or more reducing agents; one or more defoaming agents, one or more chelators; one or more zwitterionic compounds; one or more albuminoids, and one or more buffers, collectively present in an amount sufficient to denature protein, inactivate nuclease, and kill pathogens that may be contained within the biological sample; performing a PCR reaction on the mixture, wherein the reaction is initiated by contacting the mixture with a sufficient quantity of PCR reaction components to form an amplification product of a sequence that is derived from the specific nucleic acid; and detecting the presence of the amplification product in the PCR amplified mixture, wherein the presence of the amplification product is indicative of the presence of the specific nucleic acid in the biological sample.