Patent ID: 6458536
Filing Date: 2002-10-01
Classification: C07K,C12Q

Abstract:
A method of detecting a mutation or a polymorphism in the human ataxia telangiectasia gene comprising the steps of:(a) separately amplifying a plurality of nonoverlapping nucleic acid segments from the human ataxia telangiectasia gene in individual reactions; (b) subjecting the amplified nonoverlapping nucleic acid segments to single-stranded conformation polymorphism electrophoresis in a number of lanes such that two or three amplified nucleic acid segments are electrophoresed per lane, the electrophoresis of the segments electrophoresed in the same lane being initiated at different times, such that the signals from each amplified nucleic acid segment are distinct in each lane, the time interval between the initiation of electrophoresis for each segment being chosen to ensure that signals resulting from the electrophoresis are distinct for each segment electrophoresed in the same lane; and (c) comparing the signals from the resulting single-stranded conformation polymorphism electrophoresis for each segment in each of the lanes to detect the mutation or polymorphism.