Patent ID: 7235402
Filing Date: 2007-06-26
Classification: C12M

Abstract:
1. A process for the mass production of mature conifer somatic embryos in a bioreactor comprising: (a) inoculating a suitable culture medium in said bioreactor with conifer embryogenic tissues; (b) immobilizing said embryogenic tissues onto a biomass immobilizing matrix contained in said bioreactor, under initial flooding conditions by mixing said culture medium and said embryogenic tissues under low shear conditions until said embryogenic tissues attach to said biomass immobilization matrix; (c) reducing the level of said culture medium in said bioreactor to a level such that only a lower end of said matrix or less, remains immersed in said medium; and (d) subjecting said attached embryogenic tissues to a maturation step under controlled minimal humidified conditions, thereby enabling mass production of mature conifer somatic embryos.