Patent ID: 9221905
Filing Date: 2015-12-29
Classification: C07K,G01N

Abstract:
1. A method of producing an immunobinder capable of binding to an antigen of interest, wherein the immunobinder is produced by introducing a nucleic acid sequence into a host cell such that the immunobinder is produced, wherein the nucleic acid sequence encodes the immunobinder, and wherein the immunobinder-encoding nucleic acid sequence is derived from a B-cell clone identified by: (a) isolating B-cells from an animal; (b) contacting the B-cells with antigen-expressing cells expressing the antigen of interest or an artificial cell-like body displaying the antigen of interest; and, (c) isolating one or more complexes in which a B-cell is binding to an antigen-expressing cell or said artificial cell-like body displaying the antigen of interest, wherein the isolating is accomplished using a cell sorter, and wherein the B-cell in an isolated complex is identified as a B-cell clone that binds to the antigen of interest.