Patent ID: 6051427
Filing Date: 2000-04-18
Classification: A61K,C07K,C12N

Abstract:
A retroviral packaging plasmid for the production of high titers of recombinant retrovirus in human cells comprising one retroviral helper DNA sequence derived from a replication-imcompetent retroviral genome encoding in trans all virion proteins required and for packaging a replication-incompetent retroviral vector and for producing virion proteins for packaging said replication-incompetent retroviral vector at high titer, without the production of replication-competent helper virus, said retroviral DNA sequence lacking the region encoding the native enhancer and/or promoter of the viral 5' LTR of said virus and lacking both the psi function sequence responsible for packaging helper genome and the 3' LTR, and encoding a foreign enhancer and/or promoter functional in a selected mammalian cell, and a foreign polyadenylation site,wherein said helper DNA sequence codes for ecotropic Moloney murine leukemia virus (MMLV), gibbon ape leukemia virus (GALV) or human immunodeficiency virus (HIV) gag and pol, and an envelope protein or chimeric envelope protein obtained from virus selected from the group consisting of xenotropic murine leukemia virus, amphotropic murine leukemia virus, ecotropic murine leukemia virus, polytropic murine leukemia virus, 10A1 murine leukemia virus, GALV, HIV, vesicular stomatitis virus G protein, human T cell leukemia virus (HTLV) type I and HTLV type II.