Patent ID: 8158353
Filing Date: 2012-04-17
Classification: C12Q

Abstract:
1. A method for the species-specific and quantitative detection of central nervous system (CNS) tissue in meat and meat products, comprising the steps: a) preparing of the sample material and RNA extraction b) reverse transcribing of the RNA into cDNA c) analyzing of the cDNA of the glial fibrillary acidic protein (GFAP) gene in real-time PCR, wherein the real-time PCR is carried out with a pair of primers selected from the group consisting of a first pair of primers, namely SEQ ID NO 1: primer RTGcowM56F2a 5′-ACC TGC GAC CTG GAG TCC T-3′ and SEQ ID NO. 2: primer RTGcowM56R2a 5′-CTC GCG CAT CTG CCG-3′, a second pair of primers, namely SEQ ID NO. 4: primer RTGpigM56F2 5′-GAC CTG CGA CGT GGA GTC CC-3′ SEQ ID NO. 5: primer RTGpigM56R2 5′-TGG CGC TCC TCC TGC TCC-3′, and pairs of primers comprising a forward and a reverse primer having a sequence identity of at least 40% to said first or said second pair of primers; and wherein the real-time PCR is carried out using a TagMan