Patent ID: 8287700
Filing Date: 2012-10-16
Classification: B05D,C07K,G01N,Y02P

Abstract:
1. A method for preparing a functionalized matrix for oligonucleotide synthesis comprising the steps of: (a) contacting a matrix with a reagent capable of adding an amino functional group to the matrix; (b) exposing the reaction mixture of step (a) to microwave radiation thereby resulting in an amino-functionalized matrix; (c) contacting the amino-functionalized matrix of step (b) with a succinic anhydride capable of chemically succinylating the matrix and introducing a free carboxyl termini onto the amino-functionalized matrix; (d) exposing the reaction mixture of step (c) to microwave radiation thereby resulting in a succinylated matrix comprising a free carboxyl termini; and (e) coupling the succinylated matrix with a nucleoside capable of reacting with said free carboxyl termini of the succinylated matrix thereby forming a functionalized matrix for oligonucleotide synthesis.