Patent ID: 6383745
Filing Date: 2002-05-07
Classification: C07K,C12Q,Y10S

Abstract:
A method, comprising:a) providing, in any order: i) a first sample of bacteria, transfected with a recombinant expression vector comprising a nucleic acid sequence that encodes the AarC protein of SEQ ID NO:5; under conditions such that AarC protein is produced from said recombinant expression vector; ii) a second sample of bacteria, transfected with a recombinant expression vector comprising a nucleic acid sequence that encodes the AarC protein of SEQ ID NO:5; wherein said second sample comprises more recombinant expression vectors per bacteria cell than said first sample, under conditions such that more AarC protein is produced from said recombinant expression vectors than is produced in said first sample; and iii) a compound suspected of having antimicrobial activity; b) contacting said first and second samples of bacteria with said compound under conditions such that antimicrobial activity can be detected; and c) comparing the level of said antimicrobial activity of said compound on said first and second samples such that the ability of said compound to interfere with the activity of said AarC protein is determined.