Patent ID: 8980565
Filing Date: 2015-03-17
Classification: G01N,Y02A,Y10T

Abstract:
1. In a method of detecting and distinguishing first and second target compounds, cells or particles, wherein said first target is labeled with a first detectable compound and said second target is labeled with a second detectable compound, the improvement comprising: utilizing a compound comprising a first porphyrinic macrocycle as said first detectable compound and a compound comprising a second porphyrinic macrocycle as said second detectable compound; wherein each of said first and second detectable compounds are excited at an excitation wavelength band; wherein said first porphyrinic macrocycle comprises a porphyrin, chlorin, bacteriochlorin or isobacteriochlorin, and said second porphyrinic macrocycle comprises a porphyrin, chlorin, bacteriochlorin, or isobacteriochlorin; and wherein: (i) each of said first and second detectable compounds have a different emission wavelength band, said different emission wavelength bands characterized by peaks that are separated from one another by at least 5 nanometers; or (ii) said second compound has a lifetime at least 20 percent greater than said first compound.