Patent ID: 8532398
Filing Date: 2013-09-10
Classification: G01N,G02B

Abstract:
1. A method for optical segmentation of a biological sample positioned on a solid support and mounted on a fluorescent microscope comprising: transmitting light from a light source at a first predetermined wavelength on to the biological sample wherein the light causes the biological sample to fluoresce; acquiring a wide field fluorescent image of the biological sample using an image capture device; transferring the wide field fluorescent image to a digital light processor (DLP); analyzing the wide field fluorescent image utilizing, at least in part, feature based information or pixel intensity information; generating a masking pattern corresponding to a specific structure within the biological sample; transforming the masking pattern into a reformatted masking pattern, such that the said reformatted masking pattern comprises spatial coordinates of a digital micro-mirror device (DMD) and are registered to the wide field fluorescent image of the biological sample , wherein said DMD is positioned between the light source and the biological sample projecting the reformatted masking pattern onto the biological sample using the digital micro-mirror device (DMD); transmitting light from the light source at a second predetermined wavelength on to the biological sample through the reformatted mask pattern, wherein the light causes the biological sample to fluoresce; acquiring a masked fluorescent image of the biological sample with the image capture device; and converting the masked fluorescent image into a digital image.