Patent ID: 8013121
Filing Date: 2011-09-06
Classification: A61L,C08L,C12N

Abstract:
1. A method for the isolation of collagen or collagen matrix proteins from collagen-containing tissue wherein the only extractant utilized is urea, comprising the steps: a) isolation of collagen-containing fibers from the tissues; b) incubation of the isolated collagen-containing fibers in an aqueous solution containing an extractant, in which the only extractant in the aqueous solution consists of urea in a final concentration of 5-15 mol/L, in which a collagen-containing fraction is dissolved out of the fibers; c) separation of the dissolved collagen-containing fraction from the fiber and tissue residue; and d) separation of the urea from the collagen-containing fraction and renaturing of the collagen by use of gradient dialysis in aqueous PBS, so that a collagen-containing aqueous solution with isolated collagen is obtained.