Patent ID: 6818616
Filing Date: 2004-11-16
Classification: A61K,C07K,F02B

Abstract:
An isolated Neutrophil Inhibitory Factor which occurs in an Ancylostoma species and which comprises an amino acid sequence selected from the group consisting of(a) Arg-X1-X2-Phe-Leu-X3-X4-His-Asn-Gly-Tyr-Arg-Ser-X5-Leu-Ala-Leu-Gly-His-X6-X7-Ile (SEQ. ID. NO. 1), wherein X1 is Leu or Arg; X2 is Gln; Lys or Arg; X3 is Ala or Arg; X4 is Leu or Met; X5 is Lys, Arg, Leu or Ile; X6 is Val or Ile; and X7 is Ser, Gly, Asn; (b) Ala-8-X9-Ala-Ser-X10-Met-Arg-X11-Leu-X12-Tyr-Asp-Cys-X13-Ala-Glu-X14-Ser-Ala-Tyr-X15-Ser-Ala (SEQ. ID. NO. 2), wherein X8 is His or Pro; X9 is Thr, Arg or Ser; X10 is Arg or Lys; X11 is Ile or Tyr; X12 is Asp, Lys or Glu; X13 is Asp or Glu; X14 is Gly, Lys or Arg; and X15 is Glu, Met, Thr or Val; (c) Ser-X16-Phe-Ala-Asn-X17-Ala-Trp-Asp-X18-Arg-Glu-Lys-X19-Gly-Cys-Ala-Val-Val-X20-Cys (SEQ. ID. NO. 3), wherein X16 is Asn or Asp; X17 is Val or Leu; X18 is Ala or Thr; X19 is Leu, Val or Phe; and X20 is Thr, Lys or Asn; (d) His-Val-Val-Cys-His-X21-X22-Pro-Lys (SEQ. ID. NO. 4), wherein X21 is Tyr or Ile; X22 is Gly or no residue; (e) Ile-Tyr-X23-X24-Gly-X2s-Pro-Cys-X26-X27-Cys-X28-X29-Tyr (SEQ. ID. NO. 5), wherein X23 is Thr, Ser, Lys or Glu; X24 is Thr, Val or Ile; X25 is Val, Lys or Thr; X26 is Arg, Ser or Asp; X27 is Asn, Gly, Asp or Arg; X28 is Asn, Ser or Thr; and X29 is Gly, Glu or Asp; and (f) Cys-X30-X31-Asp-X32-Gly-Val-Cys-X33-Ile (SEQ. ID. NO. 6), wherein X30 is His, Ile or Asn; X31 is Ala, Pro or Asp; X32 is Glu, Val, Asp or Ile; X33 is Ile, Val or Phe; wherein said Neutrophil Inhibitory Factor is characterized as having neutrophil inhibitory activity.