Patent ID: 6284454
Filing Date: 2001-09-04
Classification: A61K,C12N,C12Q,G01N

Abstract:
A method for the detection of anti-LAV antibodies in a body fluid comprising:(A) immobilizing a predetermined amount of an antigenic peptide of LAV.sub.MAL, wherein the antigenic peptide is encoded by at least one cDNA fragment of LAV.sub.MAL produced by the process comprising treating a cDNA sequence that comprises a nucleotide sequence of FIGS. 7A-7I with at least one restriction enzyme, wherein the restriction enzyme is selected from the group consisting of AvaI, BamHI, BglII, EcoRI, HindIII, HincII, KpnI, NdeI, PstI, SacI, and XbaI;(B) contacting the immobilized LAV.sub.MAL peptide with a body fluid to be tested;(C) incubating the mixture of immobilized LAV.sub.MAL peptide and the body fluid; and(D) detecting the presence of anti-LAV antibody complexed to the immobilized LAV.sub.MAL peptide.