Patent ID: 6566141
Filing Date: 2003-05-20
Classification: C12Q,Y10T

Abstract:
A method of detecting mutation in the base sequence of a nucleic acid, comprising steps of:(A) heating a solution containing a sample double stranded nucleic acid that may be a hetero body, and thereby dissociating the double stranded nucleic acid in the sample into single stranded nucleic acid, and cooling and rebonding the same into double stranded nucleic acid; (B) increasing the temperature of the solution after completion of step (A) until the double stranded nucleic acid is dissociated into single stranded nucleic acid while measuring ultraviolet absorption of the solution and acquiring a thermal melting profile; and (C) comparing the thermal melting profile with a known thermal melting profile of a homoduplex, and determining whether a heteroduplex is present in the solution after step (A), thereby determining presence/absence of mutation in the base sequence of the double stranded nucleic acid.