Patent ID: 8828653
Filing Date: 2014-09-09
Classification: C12M,C12Q,G01N

Abstract:
1. A method for detecting microorganisms on an environmental surface, the method comprising: providing; obtaining a sample on at least one major surface of the sample collector; forming an assembly by placing the sample-collector into the sample housing with the at least one major surface comprising the sample oriented toward the cover sheet; applying the sample-suspending medium to the sample collector major surface comprising the sample; inserting the water-resistant barrier layer between the sample collector and the cover sheet, thereby preventing the sample-suspending medium from contacting at least a part of the cover sheet; incubating the assembly for a period of time; removing at least a portion of the barrier layer from the sample housing; contacting the lower major surface of the cover sheet with the sample-suspending medium to form a hydrated gel; placing a coated surface of the reagent strip in contact with the hydrated gel; incubating the sample housing for a period of time; and observing an indicator of microbial growth.