Patent ID: 9034629
Filing Date: 2015-05-19
Classification: C07C,C10L,C12N,C12P,C12Y,Y02P

Abstract:
1. An engineered cyanobacterium, wherein the engineered cyanobacterium comprises recombinant genes encoding an alkane deformylative monooxygenase having EC number 4.1.99.5, a thioesterase having EC number 3.1.2.14, and a carboxylic acid reductase having EC number 1.2.99.6, wherein the alkane deformylative monooxygenase is encoded by adm, the thioesterase is encoded by fatB or fatB2, and the carboxylic acid reductase is encoded by carB, wherein the thioesterase converts C( 8-12 ) acyl-ACP to C( 8-12 ) fatty acid, wherein the carboxylic acid reductase converts C( 8-12 ) fatty acid to C( 8-12 ) aldehyde, wherein the alkane deformylative monooxygenase converts C( 8-12 ) aldehyde to C( 7-11 ) alkane, and wherein the engineered cyanobacterium produces at least one of heptane, nonane, and undecane in an amount greater than an otherwise identical cyanobacterium, cultured under identical conditions, but lacking recombinant genes encoding alkane deformylative monooxygenase, thioesterase, and carboxylic acid reductase.