Patent ID: 7645466
Filing Date: 2010-01-12
Classification: A61K

Abstract:
1. A method for obtaining an extract composition of bio-active compounds from Fenugreek seeds, the method comprising the steps of: providing a plurality of Fenugreek seeds; soaking the Fenugreek seeds in water; crushing the Fenugreek seeds; and performing a preliminary extraction on the crushed seeds to collect a crude protein and a supernatant wherein the preliminary extraction process comprises the steps of: performing one or more extractions on the crushed Fenugreek seeds using a first solvent at a temperature between about 20° C. and about 90° C. and for a duration of between about 1 hour and about 3 hours to yield a seed residue and a seed extract; distilling the seed residue using a fractionating column by heating the seed residue until boiling, capturing, and then cooling the heated vapors derived therefrom; concentrating the distilled seed residue under vacuum to separate a Fenugreek seed oil and the first solvent; performing one or more extractions of the seed extract using a second solvent at a temperature between about 20° C. and about 90° C. and for a duration of between about 1 hour and about 3 hours to yield a second seed residue and a concentrated seed extract; subjecting the concentrated seed extract to a further concentration under vacuum to separate a second concentrated seed extract from the second solvent; cooling the second concentrated seed extract to room temperature; settling the second concentrated seed extract into the crude protein and the supernatant; and diluting the supernatant with de-ionized water to a volume between about 2 and about 10 times the volume of the supernatant, and performing a secondary extraction on the supernatant to collect a secondary extraction product comprising an amino acid content having a total weight comprising 4-hydroxyisoleucine and glutamate, wherein the 4-hydroxyisoleucine comprises an amount between 60% and about 70% of the total weight of the amino acid content and the glutamate comprises an amount between about 6% and about 9% of the total weight of the amino acid content, wherein the secondary extraction process comprises the steps of: