Patent ID: 8367890
Filing Date: 2013-02-05
Classification: A01H,C12N

Abstract:
1. A method for exchanging a target DNA sequence in the genome of a plant for a DNA sequence of interest comprising a. inducing a double stranded DNA break at a preselected site in the genome of a cell of a plant, said preselected site being located within said target DNA sequence or in the vicinity of said target DNA sequence; b. introducing a repair DNA molecule of interest into said plant cell, said DNA molecule comprising c. selecting a population of plant cells comprising said selectable or screenable marker; d. selecting a plant cell wherein said selectable or screenable marker has been introduced by homologous recombination through said flanking DNA regions and regenerating a plant from said plant cell; e. crossing said regenerated plant or a progeny plant thereof comprising said selectable marker gene with a plant comprising a chimeric gene encoding a DSBI enzyme recognizing said recognition site located in said repair DNA molecule of interest to obtain a population of F1 progeny plants, said chimeric gene comprising the following operably linked DNA segments: f. selecting an F1 progeny plant comprising said selectable or screenable marker gene and said DSBI enzyme encoding chimeric gene; g. crossing said progeny plant with a wild type plant to obtain a population of F2 progeny plants; h. selecting a population of F2 progeny plants which comprises said DSBI enzyme encoding chimeric gene; and i. selecting an F2 progeny plant wherein said selectable or screenable marker gene is deleted by homologous recombination between said one of the flanking DNA regions and a partial flanking DNA region comprising part of said one of the flanking DNA regions.