Patent ID: 8568660
Filing Date: 2013-10-29
Classification: C12M,G01N

Abstract:
1. A disk for storing liquid samples containing cells and for use in counting and observing the cells through an optical microscope, the disk comprising: an upper member formed of a transparent material and having a plurality of liquid inlets arranged radially and adapted to introduce the corresponding liquid samples therethrough, and a plurality of air vents paired with the corresponding liquid inlets; an intermediate member having a plurality of chambers extending therethrough, arranged radially and adapted to store the corresponding liquid samples therein, each chamber corresponding to each pair of the liquid inlet and the air vent and communicating with the liquid inlet and with the air vent; and a lower member formed of a transparent material and backing the intermediate member, the lower member presenting a first surface exposed to the liquid samples within the chambers; and wherein the upper member presents a second surface exposed to the liquid samples within the chambers and facing the first surface, the first and second surfaces being located on opposing sides of the intermediate member; and wherein a transparent hydrophilic film forms the second surface of the upper member, or forms the second surface of the upper member and the first surface of the lower member, the hydrophilic film/films causing the liquid samples introduced through the liquid inlets to be uniformly distributed against surface tension within the chambers.