Patent ID: 6359683
Filing Date: 2002-03-19
Classification: G01N

Abstract:
A method for determining the volume of particles that are suspended in a liquid sample comprising:a) depositing said liquid sample that contains suspended particles into an optical cuvette having an entrance window and an output window at a known distance (X0) from each other; b) adding and evenly distributing a light-absorbing dye into the liquid sample that does not leak into the suspended particles; c) sending light of such a wavelength through the cuvette that said light is highly absorbed by the added dye, but only weakly absorbed by the suspended particles; d) measuring the optical transmission through the cuvette in an area that contains no particles (I1/I0); e) measuring the optical transmission through the cuvette in an area that contains particles (I3/I0â€²); and f) calculating the volume of the suspended particles based on these transmission values and the known distance of the cuvette windows.