Patent ID: 6184023
Filing Date: 2001-02-06
Classification: A61K,C12N,G03C,Y10S

Abstract:
A purified chondroitinase ABC having the following characteristics:(i) a single polypeptide having a molecular weight of about 100,000 daltons, as measured by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), in a reduced or unreduced state, and by gel permeation chromatography,(ii) isoelectric points of about 8.2 about 8.5,(iii) an optimum pH of about 8.0 to 8.2,(iv) an optimum reaction temperature of about 30 to 42.degree. C.,(v) a specific activity of at least 300 U/mg, and(vi) a single peak as determined by HPLC;wherein activity of the chondroitinase ABC is inhibited by a metal ion selected from the group consisting of Zn.sup.2+, Ni.sup.2+, Fe.sup.3+, and Cu.sup.2+, andwherein the purified chondroitinase ABC is essentially free of nucleic acid, endotoxin and protease activity, and has alanine as the N-terminal amino acid and proline as the C-terminal amino acid.