Patent ID: 9121047
Filing Date: 2015-09-01
Classification: B01F,B01L,B04B,C12M,C12P,C12Q

Abstract:
1. A method of generating an amplified sample, the method comprising: generating an emulsion from a carrier fluid and a sample fluid with an emulsion generator, the emulsion generator including a carrier fluid port to receive the carrier fluid, a sample fluid port to receive the sample fluid, and an emulsion outlet port, the emulsion generator including a membrane to generate an emulsion including the carrier fluid and the sample fluid, the emulsion exiting the emulsion generator via the emulsion outlet port, the sample fluid including conjugated beads; heating the emulsion to an amplification condition with a heater of an amplification device, the amplification device including an inlet port in fluid communication with the emulsion outlet port and to receive the emulsion, the amplification device including an effluent port, the amplified emulsion exiting the amplification device via the effluent port, the amplified emulsion including amplified beads derived from the conjugated beads; and breaking the emulsion following heating with a centrifuge device, the centrifuge device including an inlet conduit in fluid communication with the effluent port and to receive the emulsion after heating, the centrifuge device further including a rotor to receive a tube and a slinger to dispense the amplified emulsion received via the inlet conduit to the tube when the rotor is spinning, the amplified beads being dispensed to the tube.