Patent ID: 6620609
Filing Date: 2003-09-16
Classification: C12N,Y10S

Abstract:
A process of using microwave sterilization for the preparation of a sterile culture medium for the selection of recombinant microorganisms using the action of microwaves, said process consisting of the steps of:a. adding a selection agent and/or identification agent, and further adding a heat protective agent, to a suitable culture medium containing suitable culturing ingredients, wherein said adding step is carried out prior to microwave sterilization; b. mixing all said culturing ingredients in said suitable culture medium with said selection and/or identification agent, and heat protective agent, said culturing ingredients and said selection and/or identification agent and heat protective agent being in powder form, so as to obtain a mixture in powder form; c. mixing said mixture in powder form with a sufficient quantity of water to obtain a suspension of said powder in water; and d. subjecting said suspension of powder dissolved in water to heat by the action of microwaves in order to sterilize said suspension without raising pressure above ambient pressure by not raising temperature of said suspension above its boiling temperature and allow said suspension to become homogeneous.