Patent ID: 9175421
Filing Date: 2015-11-03
Classification: B01J,B01L,C40B,G01N

Abstract:
1. A method for determining one or more kinetic parameters of binding between a first binding member and a second binding member comprising: (a) adsorbing a first binding member to a surface of a plurality of microspots, wherein the adsorption occurs simultaneously in all microspots; (b) presenting a second binding member to the first binding member simultaneously to each of the microspots, there being a plurality of combinations of first binding member surface density and second binding member concentration among the plurality of microspots; (c) obtaining data indicative of a binding reaction between the first and second binding members simultaneously from each of the plurality of microspots by a biosensor detection method; and (d) processing the data so as to obtain one or more kinetic parameters of binding between the first and second binding members; wherein the plurality of binding reactions does not necessitate a regeneration stage, and wherein in step (a) adsorbing the first binding member to a surface at a plurality of microspots comprises: (a) activating the surface in the microspot by presenting thereto a chemical activating substance by: (b) adsorbing the first binding member to the microspot; and (c) deactivating the microspot.