Patent ID: 7083795
Filing Date: 2006-08-01
Classification: A61K,A61P,C12N,G01N

Abstract:
1. A method of preparing a non-immunopotentiating, vaccine composition capable of protecting a human host immunized therewith against disease caused by infection by respiratory syncytial (RS) virus, which comprises: growing RS virus on a continuous cell line of vaccine quality in the presence of microcarrier beads to produce a grown virus; harvesting said growth virus by collecting culture fluid to produce a harvested virus; purifying said harvested virus under non-denaturing conditions to produce a purified virus free from cellular and serum components; inactivating said purified virus with an inactivating agent selected from the group consisting of β-propiolactone, a non-ionic detergent which is n-octyl-α-D-glucopyranoside or n-octyl-β-D-glucopyranoside, and ascorbic acid to provide a non-infectious, non-immunopotentiating and protective RS viral preparation, and formulating said non-infectious, non-immunopotentiating and protective RS viral preparation as a vaccine.