Patent ID: 6388168
Filing Date: 2002-05-14
Classification: C12N

Abstract:
An expression cassette suitable for use in higher plants for producing a chimeric gene for expression in stably transformed plastids having genomes containing said chimeric gene, which comprises:a) a 5â€² regulatory segment for controlling expression of a coding segment in plastids; b) a 3â€² regulatory segment for promoting stability of mRNA produced during said 5â€² regulatory segment-controlled expression of said coding segment in said plastids; and c) a cloning segment operably linked to said 5â€² regulatory segment and said 3â€² regulatory segment, said cloning segment being adapted for insertion of a coding segment, such that said expression of said inserted coding segment is controlled by said 5â€² regulatory segment and said mRNA produced during said expression is stabilized by said 3â€² regulatory segment, wherein said 5â€² regulatory segment comprises a nucleotide sequence selected from the group consisting of SEQ ID NO: 5, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, and SEQ ID NO: 17.