Patent ID: 7803991
Filing Date: 2010-09-28
Classification: C12N,Y02A

Abstract:
1. A stably transformed herbicide resistant target plant species, or the progeny thereof, which comprises a chloroplast stably transformed with a universal integration and expression vector competent for stable transformation of the chloroplast of different plant species, which vector comprises an expression cassette which encodes a heterologous protein of interest expressed by a heterologous DNA sequence in the chloroplast genome of the target plant species, which heterologous DNA confers tolerance to said herbicide, and flanking each side of the expression cassette, flanking DNA sequences derived from a transcriptionally active spacer region of a chloroplast genome, whereby stable integration of the heterologous DNA encoding the heterologous protein into a transcriptionally active spacer region of the chloroplast genome of the target plant was facilitated through homologous recombination of the flanking sequences with the homologous sequences in the target chloroplast genome.