Patent ID: 6183956
Filing Date: 2001-02-06
Classification: C12Q

Abstract:
A method of measuring the expression of a selected RNA in the presence of a potential nucleic acid transcriptional activity modulator in a sample, the method comprising the steps of:(i) providing a sample comprising a potential transcriptional activity modulator and an expression cassette comprising a promoter operably linked to a selected nucleic acid encoding the selected RNA;(ii) incubating the sample with a DNA oligonucleotide, wherein a region of the oligonucleotide is complementary to a region of the selected RNA, forming an RNA:DNA heteroduplex;(iii) cleaving single-stranded RNA in the sample with an RNase enzyme;(iv) binding the RNA:DNA heteroduplex to a first recognition reagent comprising an antibody that binds to RNA:DNA heteroduplexes, the antibody immobilized on a solid substrate, thereby immobilizing the RNA:DNA heteroduplex on the solid substrate; and(v) detecting the RNA:DNA heteroduplex bound to the solid substrate.