Patent ID: 7081563
Filing Date: 2006-07-25
Classification: A23K,A23L,C12N,C12Y

Abstract:
1. A method to prepare a transformed plant which expresses a thermotolerant phytase comprising: a) introducing into a plant cell an expression cassette comprising a promoter operably linked to a nucleic acid molecule encoding a thermotolerant phytase which retains at least 40% activity after 30 minutes at 60° C. and has a specific activity of greater than 200 U/mg at pH 4.5 and 37° C. so as to yield a transformed plant cell; and b) obtaining a transgenic plant from the transformed plant cell, which plant expresses the thermotolerant phytase in the cells of the plant, and wherein the thermotolerant phytase comprises the polypeptide sequence depicted in amino acids 20–431 of SEQ ID NO:4 or a conservative variant thereof.