Patent ID: 6045990
Filing Date: 2000-04-04
Classification: A01N,A61P

Abstract:
A cell-free solution composition for hypothermic storage of animal or human organs, tissues or cells, the cell-free solution comprising:(a) one or more electrolytes selected from the group consisting of potassium ions at a concentration ranging from 35-45 mM, sodium ions ranging from 80-120 mM, magnesium ions ranging from 2-10 mM, and calcium ions ranging from 0.01-0.1 mM;(b) a macromolecular oncotic agent having a size sufficiently large to limit escape from the circulation system and effective to maintain oncotic pressure equivalent to that of blood plasma and selected from the group consisting of human serum albumin, polysaccharide and colloidal starch;(c) a biological pH buffer effective under physiological and hypothermic conditions;(d) a nutritive effective amount of at least one simple sugar;(e) an impermeant and hydroxyl radical scavenging effective amount of mannitol;(f) an impermeant anion impermeable to cell membranes and effective to counteract cell swelling during cold exposure, said impermeant ion being at least one member selected from the group consisting of lactobionate, gluconate, citrate and glycerophosphate;(g) a substrate effective for the regeneration of ATP, said substrate being at least one member selected from the group consisting of adenosine, fructose, ribose and adenine, and(h) at least one agent which regulates apoptotic induced cell death.