Patent ID: 8491904
Filing Date: 2013-07-23
Classification: A61K,A61P,C07K

Abstract:
1. A method for producing a host cell-protein (HCP) reduced antiIL-13 antibody, or antigen-biding fragment thereof, preparation from a sample mixture comprising an anti-IL-13 antibody, or antigen-binding fragment thereof, and at least one HCP, said method comprising: (a) contacting said sample mixture to Protein A affinity chromatography resin, washing said affinity chromatomphy resin with a buffer comprising 0.5 M NaCl, 20 mM Na citrate, at pH 6, and a buffer comprising 25 mM Tris, 100 mM NaCl, pH 7.2 and collecting an affinity chromatography sample; (b) subjecting said affinity chromatography sample to a reduction in pH thus forming a reduced pH sample, wherein said reduction in pH is from about 3 to about 4; (c) adjusting said reduced pH sample to a pH of about 4.5 to about 6.5 and contacting said adjusted pH sample to an ion exchange resin and collecting an ion exchange sample; (d) contacting said ion exchange sample to a hydrophobic interactive chromatography (HIC) resin and collecting an HIC sample, wherein said HIC sample comprises said HCP-reduced antibody, or antigen binding portion thereof, preparation.