Patent ID: 7943089
Filing Date: 2011-05-17
Classification: G01N

Abstract:
1. An assay device for detecting the presence or quantity of an analyte residing in a test sample, the assay device comprising: a first substrate onto which is printed a detection working electrode capable of generating a measurable detection current and a calibration working electrode capable of generating a measurable calibration current, wherein the detection working electrode has a thickness of less than about 100 micrometers, the detection working electrode comprising an immobilized immunoreactive specific binding capture ligand for the analyte, the calibration working electrode being generally configured to not specifically bind the analyte; a second substrate onto which is printed an auxiliary electrode selected from the group consisting of a counter electrode, a reference electrode, and combinations thereof; a multi-channel potentiostat, the multi-channel potentiostat capable of receiving the detection current from the detection working electrode and the calibration current from the calibration working electrode; and wherein the first and second substrates are laminated together so that the auxiliary electrode is positioned adjacent to the detection working electrode and the calibration working electrode, wherein the detection current calibrated by the calibration current is proportional to the amount of the analyte within the test sample.