Patent ID: 7908094
Filing Date: 2011-03-15
Classification: A21D,C12N

Abstract:
1. A method of constructing a variant of a parent maltogenic alpha-amylase, wherein said parent maltogenic alpha-amylase has the amino acid sequence of amino acid residues 1-686 of SEQ ID NO:2, and wherein said parent maltogenic alpha-amylase has a substrate binding site which is formed by amino acid residues corresponding to residues 37-45, residues 261-266, residues 327-330, residues 370-376, residues 135-145, residues 173-180 and residues 188-196 in SEQ ID NO:2, which method comprises: a) modeling the parent alpha-amylase on the three-dimensional structure of amino acid residues 1-686 of SEQ ID NO:2 depicted in the Appendix to produce a three-dimensional structure of the parent alpha-amylase; b) identifying in said three-dimensional structure of the parent alpha-amylase an amino acid residue which (i) is within 6 Å of said substrate binding site and (ii) is involved in interactions with a bound substrate; and c) constructing the variant by substituting the amino acid residue identified in said b) with another amino acid residue which changes the substrate specificity of said substrate binding site, and wherein the variant has maltogenic alpha-amylase activity.