Patent ID: 7871817
Filing Date: 2011-01-18
Classification: A61P,C07H,C07K,C12N

Abstract:
1. An in vitro method for producing a T-cell expressing a T-cell receptor (TCR) comprising the steps of: (a) providing DNA molecules encoding the alpha-chain and the beta-chain of a TCR specific for an antigen, said TCR being a human TCR, murine TCR or a humanized murine TCR; (b) altering the sequence of the DNA molecule encoding the alpha-chain of said TCR to exchange Gly 192 of the alpha-chain constant region for an arginine residue; (c) altering the sequence of the DNA molecule encoding the beta-chain of said TCR to exchange Arg208 of the beta-chain constant region with a glycine residue; and (d) transfecting, in vitro, a T-cell with said mutagenized DNA molecules and expressing said mutagenized TCR.