Patent ID: 6238867
Filing Date: 2001-05-29
Classification: C12N,G01N

Abstract:
A first ribonucleic acid (RNA) molecule and a second RNA molecule for use in binding an RNA-binding protein having a protein component and a RNA component, said first RNA molecule capable of binding to said RNA-binding protein and having the following formula:5'-A-B-C-3';wherein A is a section of the RNA molecule having 10-100,000 nucleotides which section is capable of being received by an RNA replicase and with another RNA sequence, F, being replicated, the letter ""B"" denotes a section of the RNA molecule having approximately 10 to 3,000 nucleotides which have affinity to one consensus sequence of said RNA-binding protein and which are capable of binding to said protein component, and the letter ""C"" denotes a section of the RNA molecule having approximately 1 to 20 nucleotides which section is capable of being ligated to another RNA sequence, ""D"" of the second RNA molecule: the second RNA molecule capable of binding to said RNA-binding protein and having the following formula:5'-D-E-F-3';wherein D is a section of the RNA molecule having approximately 1 to 20 nucleotides which section is capable being ligated with another RNA sequence, ""C"", the letter ""E"" denotes a section of the RNA molecule having approximately 10 to 3,000 nucleotides which have affinity to second consensus sequence of said RNA-binding protein and which are capable of binding to said protein component, and the letter ""F"" denotes a section of the RNA molecule having 10 to 100,000 nucleotides which section is capable of being received by an RNA replicase and with another sequence, ""A"", being replicated; said the first and the second RNA molecules are capable of forming a third RNA molecule having the following formula:5'-A-B-C-D-E-F-3';said third RNA molecule formed by ligating the C and D sections, as the E and the B sections are bound to said two consensus sequences of the same RNA-binding protein, said third RNA capable of being received by an RNA replicase and being replicated by such enzyme.