Patent ID: 8309317
Filing Date: 2012-11-13
Classification: C07K,C12N,Y02A

Abstract:
1. A method of obtaining protein and nucleic acid sequence information about proteins produced from isolated cells of interest, comprising the steps of: placing a plurality of individual cells in a plurality of individual wells, wherein each cell produces at least one protein of interest; contacting said at least one protein of interest in the wells with a first surface comprising at least one protein binding agent, which selectively binds at least one of the proteins of interest and which first surface comprises addressable regions that are relatable to the individual wells; determining one or more properties of said one or more proteins of interest and relating the one or more properties to a particular addressed region of the first surface thereby identifying a well of particular interest; lysing the cells in the wells; hybridizing nucleic acids from the lysed cells with oligonucleotide probes comprising tags bound to a second surface, wherein the second surface comprises regions relatable to the individual wells and wherein the tags in each region are different; converting the nucleic acids into copy nucleic acids that comprise the tags specific to the regions on the second surface; pooling the tagged copy nucleic acids; sequencing the tagged copy nucleic acids; and utilizing the tags to associate the copy nucleic acid sequences to a particular well, which associates the copy nucleic acid sequences with properties of the proteins of interest.