Patent ID: 8535914
Filing Date: 2013-09-17
Classification: C12Q

Abstract:
1. A kit for conducting at least one of detection and diagnosis of gastric cancer, comprising: an array consisting of eleven probes immobilized on a solid support, the eleven probes being used for detecting eleven target genes; and a plurality of primer sets for amplifying by PCR a partial region of each of the eleven target genes, wherein the eleven target genes consist of: ten genes specific to gastric cancer cells selected from the group consisting of: trefoil factor 1 (TFF1); trefoil factor 2 (TFF2); fatty acid binding protein 1, liver (FABP1); cytokeratins 20 (CK20); mucin 2 (MUC2); carcinoembryonic antigen (CEA); tumor-associated calcium signal transducer 1 (TACSTD1); maspin (MASPIN); protease, serine, 4, (PRSS4); and olfactomedin 4 (GW112); and beta-actin (ACTB) which is a housekeeping gene and with which expression amounts of the other genes are compared, and wherein the kit comprises each of the following eleven combinations, each comprising one of the eleven probes and one of the plurality of primer sets: (Combination for detecting TFF1): Combination of a probe for detecting TFF1 consisting of a nucleotide sequence of SEQ ID NO: 1 of the sequence listing and a primer set for amplifying TFF1 consisting of nucleotide sequences of SEQ ID NOS: 12 and 13 of the sequence listing, (Combination for detecting TFF2): Combination of a probe for detecting TFF2 consisting of a nucleotide sequence of SEQ ID NO: 2 of the sequence listing and a primer set for amplifying TFF2 consisting of nucleotide sequences of SEQ ID NOS: 14 and 15 of the sequence listing, (Combination for detecting FABP1): Combination of a probe for detecting FABP1 consisting of a nucleotide sequence of SEQ ID NO: 3 of the sequence listing and a primer set for amplifying FABP1 consisting of nucleotide sequences of SEQ ID NOS: 16 and 17 of the sequence listing, (Combination for detecting CK20): Combination of a probe for detecting CK20 consisting of a nucleotide sequence of SEQ ID NO: 4 of the sequence listing and a primer set for amplifying CK20 consisting of nucleotide sequences of SEQ ID NOS: 18 and 19 of the sequence listing, (Combination for detecting MUC2): Combination of a probe for detecting MUC2 consisting of a nucleotide sequence of SEQ ID NO: 5 of the sequence listing and a primer set for amplifying MUC2 consisting of nucleotide sequences of SEQ ID NOS: 26 and 27 of the sequence listing, (Combination for detecting CEA): Combination of a probe for detecting CEA consisting of a nucleotide sequence of SEQ ID NO: 6 of the sequence listing and a primer set for amplifying CEA consisting of nucleotide sequences of SEQ ID NOS: 20 and 21 of the sequence listing, (Combination for detecting TACSTD1): Combination of a probe for detecting TACSTD1 consisting of a nucleotide sequence of SEQ ID NO: 7 of the sequence listing and a primer set for amplifying TACSTD1 consisting of nucleotide sequences of SEQ ID NOS: 22 and 23 of the sequence listing, (Combination for detecting MASPIN): Combination of a probe for detecting MASPIN consisting of a nucleotide sequence of SEQ ID NO: 8 of the sequence listing and a primer set for amplifying MASPIN consisting of nucleotide sequences of SEQ ID NOS: 32 and 33 of the sequence listing, (Combination for detecting PRSS4): Combination of a probe for detecting PRSS4 consisting of a nucleotide sequence of SEQ ID NO: 9 of the sequence listing and a primer set for amplifying PRSS4 consisting of nucleotide sequences of SEQ ID NOS: 28 and 29 of the sequence listing, (Combination for detecting GW112): Combination of a probe for detecting GW112 consisting of a nucleotide sequence of SEQ ID NO: 10 of the sequence listing and a primer set for amplifying GW112 consisting of nucleotide sequences of SEQ ID NOS: 24 and 25 of the sequence listing, (Combination for detecting ACTB): Combination of a probe for detecting ACTB consisting of a nucleotide sequence of SEQ ID NO: 11 of the sequence listing and a primer set for amplifying ACTB consisting of nucleotide sequences of SEQ ID NOS: 30 and 31.