Patent ID: 6077667
Filing Date: 2000-06-20
Classification: A01K,C12N

Abstract:
A method for deleting a selected region of genetic material in cells comprising the steps of:inserting a first selection cassette at a 5' end of said selected region using conventional gene targeting methods, said first selection cassette comprising a first selectable marker, a first loxP recombination site, and a first portion of a second selectable marker;selecting cells expressing said first selectable marker;inserting a second selection cassette at a 3' end of said selected region using conventional gene targeting methods, said second selection cassette comprising a third selectable marker, a second loxP recombination site, and a remaining portion of said second selectable marker;expressing Cre recombinase to produce recombination between said first and second loxP sites; andselecting cells expressing said second selectable marker,wherein the selected region of genetic material is deleted.