Patent ID: 8956851
Filing Date: 2015-02-17
Classification: C12N,C12P,C12Y,Y02E

Abstract:
1. A recombinant microorganism comprising: (a) a deletion of one or more native enzymes that function to produce glycerol and/or regulate glycerol synthesis, wherein said one or more enzymes is encoded by a glycerol-3-phosphate dehydrogenase (gpd1), glycerol-3-phosphate dehydrogenase (gpd2) glycerol 3-phosphatase 1′, glycerol 3-phosphatase 2 or fps1 polynucleotide; and (b) one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source to ethanol, wherein one of said metabolic pathways comprises conversion of pyruvate to acetyl-CoA and formate by a pyruvate formate lyase, wherein one of said metabolic pathways comprises conversion of acetyl-CoA to ethanol by an acetaldehyde dehydrogenase, alcohol dehydrogenase, or a bifunctional acetaldehyde/alcohol dehydrogenase, and wherein said one or more native and/or heterologous enzymes is activated, upregulated or downregulated.