Patent ID: 6010892
Filing Date: 2000-01-04
Classification: A23K,C12C,C12H,C12N,C12P,D21C

Abstract:
An animal feed composition which contains a protein having acetyl xylan esterase activity wherein said protein is produced by culturing a microbial host modified to contain an expression system for said protein under conditions that give rise to the production of said protein and optionally recovering the protein from the culture wherein said protein is encoded by nucleotide sequence which(a) encodes the protein comprising the amino acid sequence of positions 26-304 in SEQ ID NO:8; or(b) encodes a modified protein which differs by one or more amino acids from positions 26-304 of SEQ ID NO:8 having acetyl xylan esterase activity that is encoded by a nucleotide sequence which hybridizes with the nucleotide sequence of SEQ ID NO:7 under the hybridization conditions of a hybridization buffer which is 6.times.SSC, 0.5% SDS and 10.times.Denhardt's at a starting temperature of 65.degree. C. and a final temperature of 47.degree. C., followed by washing in 2.times.SSC, followed by washing in said hybridization buffer at 47.degree. C., followed by two washes for 30 minutes at 56.degree. C. in 6.times.SSC, 0.05% pyrophosphate.