Patent ID: 7521176
Filing Date: 2009-04-21
Classification: G01N,Y10T

Abstract:
1. A method of detecting lymphocytes expressing cell-surface gp120 in an aqueous sample containing viral infected cells displaying gp120, comprising: a. combining to form a mixture: b. incubating said mixture under conditions effective for (i) binding of said monoclonal antibodies to gp120 on said cells, and (ii) for binding of said second antibodies to said detectable labels attached to said monoclonal antibodies, to form a complex, wherein each of said monoclonal antibodies is bound to said gp120 displayed on a viral infected cell; c. separating said complex by applying a magnetic field to said mixture, whereby said complex is retained by said magnetic field, and d. determining the presence of magnetically separated lymphocytes expressing cell-surface gp120.