Patent ID: 6015661
Filing Date: 2000-01-18
Classification: C12Q,G01N

Abstract:
A method for detecting a non-pathogenic HIV-1 strain in the body fluids of an HIV infected individual comprising:(a) obtaining and preparing a biological sample from said HIV infected individual;(b) admixing an aliquot of said sample with an HIV-1.sub.NL43 Nef peptide consisting of amino acids 166-206;(c) admixing an aliquot of said sample with an HIV-1.sub.NL43 Nef peptide wherein said peptide contains a known HIV-1 antigenic determinant but excludes amino acids 166-206 of Nef;(d) detecting the formation of antigen/antibody complexes of the samples of steps (a) and (b);wherein the absence of immune complex formation in step (b) and the presence of immune complex formation in step (c) is indicative of said individual being infected with a non-pathogenic HIV-1 strain.