Patent ID: 6818397
Filing Date: 2004-11-16
Classification: C07H,C12Q,Y10S

Abstract:
A kit for detecting and differentiating an enterovirus in a sample, comprising at least one pair of oligonucleotide primers for nucleic acid amplification, wherein a first primer of said pair consists of a sequence of any of:SEQ ID NO: 1: TTGTRCGCCTGTTTTA, SEQ ID NO: 2: CAAGCACTTCTGTHHCCCCGG, SEQ ID NO: 3: TACTTCGAGAARCCYAGTA, SEQ ID NO: 4: AAGAGYCTATTGAGCTA, or SEQ ID NO: 5: GGITGGTRSTGGAARTTICC, or a degenerate sequence of SEQ ID No: 5; and a second primer of said pair consists of a sequence of any of: SEQ ID NO: 6: CACYGGATGGCCAATCCAA, SEQ ID NO: 7: ATTGTCACCATAAGCAGCCA, or SEQ ID NO: 8: ARRTTIATCCAYTGRTGIGG, or a degenerate sequence of SEQ ID No: 8, provided that the second primer consists of the sequence of SEQ ID NO: 8 or a degenerate sequence of SEQ ID NO: 8 when the first primer consists of the sequence of SEQ ID NO: 5 or a degenerate sequence of SEQ ID NO: 5; and at least one synthetic nucleotide sequence fixed on a solid substrate for nucleic acid hybridization with nucleic acids obtained from the amplification, wherein the synthetic nucleotide comprises any sequence selected from the group consisting of: SEQ ID NO: 9: TCCTCCGGCCCCTGAATGCGGCTAATC, SEQ ID NO: 10: TGTCGTAACGSGCAASTCYGYRGCGGAACCGAC, SEQ ID NO: 11: TACTTTGGGTGTCCGTGTTTCHTTTTAT, SEQ ID NO: 12: CTTATAAGCAGACTCAACCCGGTGCTGATG, SEQ ID NO: 13: TGGCATTCCAATATCACAATTAACAGTG, SEQ ID NO: 14: CTCGGCACTATCGCAGGAGGGACCGGGAAT or SEQ ID NO: 15: CCTACGCCACTACACAGCCTGGTCAGGTTG, or a degenerate sequence of any of SEQ ID Nos.: 12-15.