Patent ID: 8486707
Filing Date: 2013-07-16
Classification: C12Q,H01J,Y10T

Abstract:
1. A computer-implemented method for confirming the nucleotide sequence of an oligonucleotide comprising: a) inputting the nucleotide sequence of an oligonucleotide; b) executing, using a computer, an algorithm that provides the predicted molecular formulas of fragments of the oligonucleotide, wherein the algorithm provides predicted molecular formulas for all a-B, a, b, c, d, w, x, v and z fragments of the oligonucleotide, where the bonds broken to obtain said fragments are defined in c) predicting exact m/z values of said fragments using said molecular formulas; and d) comparing the predicted exact mass to m/z values of c) to experimentally-obtained m/z values obtained by analysis of said oligonucleotide by tandem mass spectrometry to determine if the predicted exact mass to m/z values correlate with the experimentally-obtained m/z values.