Patent ID: 7691629
Filing Date: 2010-04-06
Classification: A61K,A61P,C12N

Abstract:
1. A method of culturing human neural stem cells, said method comprising: providing human neural stem cells; incubating a culture vessel with an about 0.1 μg/mL to about 1 mg/mL solution of poly-D-lysine for about 5 minutes to about 3 hours; washing and drying the culture vessel to form a pre-coated culture vessel; seeding the neural stem cells in said pre-coated culture vessel in absence of serum; adding a fibronectin solution and at least one mitogen to the pre-coated culture vessel and culturing the neural stem cells in absence of serum; further culturing the neural stem cells with at least one mitogen; and passaging the cultured neural stem cells prior to confluence.