Patent ID: 6780606
Filing Date: 2004-08-24
Classification: G01N

Abstract:
A method for determining the occurrence of a cerebral event and differentially diagnosing between an ischemic cerebral event and a hemorrhagic cerebral event comprising:a. analyzing a body fluid of a patient to detect presence and concentration level of ischemic marker proteins, said ischemic marker proteins consisting of myelin basic protein (MBP), the beta isoform of S 100 protein (S 100), and neuronal specific enolase (NSE), said analyzing comprising contacting said MBP, the beta isoform of S100 and NSE with a reagent capable of detecting said marker proteins, detecting each of said marker proteins, and removing reagent that does not detect said marker proteins, b. analyzing a body fluid of said patient to detect presence and concentration level of a brain endothelial cell membrane protein, said analyzing comprising contacting said brain endothelial cell membrane protein with a reagent capable of detecting said endothelial cell membrane protein, detecting said endothelial cell membrane protein, and removing reagent that does not detect said brain endothelial cell membrane protein, c. comparing the concentration level of each protein detected in steps (a) and (b) to specific threshold values to determine the presence of statistically significant concentrations thereof, d. assessing patient condition by comparing said presence or absence of statistically significant concentrations of said protein in accordance with an analytical flowchart; and e. determining whether the patient condition assessed in step (d) is an ischemic cerebral event or an hemorrhagic cerebral event, wherein if only said NSE is elevated, or if only said brain endothelial cell membrane protein is elevated, or if S100 is present alone or along with any of NSE, MBP or a brain endothelial cell membrane, then said patient condition is indicative of an ischemic cerebral event; unless MBP is present at a level 200 times normal or greater, or unless S100 and NSE levels are elevated, and MBP and brain endothelial membrane protein levels are normal, then said patient condition is indicative of a hemorrhagic cerebral event.