Patent ID: 8389699
Filing Date: 2013-03-05
Classification: C12Q

Abstract:
1. A kit for sequencing a polynucleotide sequence, the kit comprising: (a) a cationically charged nucleic acid terminator, the cationically charged nucleic acid terminator comprising a labeled compound of structure (I): wherein Z is selected from the group consisting of a mono-phosphate, a di-phosphate, a tri-phosphate, a thiophosphate, and a boranophosphate; wherein L is substituted with a cationic moiety comprising more than one charged units that are covalently linked together which imparts a positive charge to structure (I), and wherein at least one of L, B, S, X, or Z is substituted with a reporter moiety; each of the charged units is an imidazolium moiety; and (b) a discriminatory polymerase that is exonuclease minus.