Patent ID: 6699693
Filing Date: 2004-03-02
Classification: C12Q

Abstract:
A method for replication of a target region of a target DNA molecule comprising the steps of:(a) introducing a D-Loop into the target duplex DNA molecule at a first initiation point adjacent to the target region in a reaction mixture, wherein the step of introducing a D-loop is performed by hybridizing the duplex DNA molecule with a first oligonucleotide primer which is substantially complementary to the first initiation site; (b) adding proteins to the reaction mixture to assemble a replisome at the D-loop; and (c) providing DNA monomers and ATP to the replisome, whereby the target region is reproduced, and further comprising the step of introducing a second D-loop by hybridizing the duplex DNA molecule with a second oligonucleotide primer which is substantially complementary to a second initiation site, said target region lying between the first and second initiation sites.