Patent ID: 6248568
Filing Date: 2001-06-19
Classification: C07H,C12Q

Abstract:
A method for performing a primer extension reaction comprising the steps of:providing a template nucleic acid;annealing an oligonucleotide primer to a portion of the template nucleic acid; andadding primer-extension reagents to the primer-template hybrid for extending the primer, the primer extension reagents including a nucleoside compound having the structure: ##STR12##wherein:R.sub.1 and R.sub.2 taken separately are selected from the group consisting of --H, lower alkyl, protecting group, and label;B is a 7-deazapurine, purine, or pyrimidine nucleoside base;wherein when B is purine or 7-deazapurine, the sugar moiety is attached at the N.sup.9 -position of the purine or deazapurine, and when B is pyrimidine, the sugar moiety is attached at the N.sup.1 -position of the pyrimidine; andwherein if B is a purine, the adjacent triple-bonded carbon is attached to the 8-position of the purine if B is 7-deazapurine, the adjacent triple-bonded carbon is attached to the 7-position of the 7-deazapurine, and if B is pyrimidine, the adjacent triple-bonded carbon is attached to the 5-position of the pyrimidine;W.sub.1 is selected from the group consisting of --H and --OH;W.sub.2 is --OH or a moiety which renders the nucleoside incapable of forming a phosphodiester bond at the 3'-position; andW.sub.3 is selected from the group consisting of --PO.sub.4, --P.sub.2 O.sub.7, --P.sub.3 O.sub.10, phosphate analog, and --OH.