Patent ID: 9090920
Filing Date: 2015-07-28
Classification: C12N,C12P

Abstract:
1. A microorganism selected from Gluconobacter oxydans wherein a gene encoding a polypeptide with the activity of a repressor of L-sorbosone dehydrogenase (SNDH) and L-sorbose dehydrogenase (SDH) is disrupted, said gene comprising a polynucleotide selected from the group consisting of: (a) polynucleotides encoding a polypeptide comprising the amino acid sequence of which is represented by SEQ ID NO:2; (b) polynucleotide comprising the nucleotide sequence according to SEQ ID NO:1; (c) polynucleotide the complementary strand of which hybridizes under highly stringent conditions to a polynucleotide as defined in (a) or (b) and which encodes a polypeptide with the activity of a repressor of SNDH and SDH, wherein said highly stringent conditions comprise hybridization at 42° C. for 2 to 4 days followed by one or more washes in 2×SSC, 0.1% SDS at room temperature and one or more washes in 0.5×SSC, 0.1% SDS or 0.1×SSC, 0.1% SDS at 65 to 68° C.; wherein said microorganism produces at least 10% more Vitamin C and/or 2-KGA from L-sorbose compared to the respective microorganism wherein said repressor is intact.