Patent ID: 7371514
Filing Date: 2008-05-13
Classification: G01N

Abstract:
1. A method to perform a mass analysis of a polypeptide comprising: (a) derivatizing the polypeptide by reacting the polypeptide with an imidazole to yield a derivatized polypeptide having an imidazole derivative of lysine; (b) derivatizing the polypeptide by reacting the polypeptide with an alkylating agent to yield a derivatized polypeptide fragment having an alkyl derivative of the carboxyl group of an acidic side chain of glutamic acid or aspartic acid, wherein the above derivatizing steps yield a serially derivatized polypeptide having both an imidazole derivative of the lysine and the alkylated carboxyl group; and (c) obtaining a mass analysis of the serially derivatized polypeptide.