Patent ID: 6946252
Filing Date: 2005-09-20
Classification: B01L

Abstract:
1. A sample preparation method for DNA analysis comprising the steps of: amplifying, at the same time and under the same condition, a plurality of target DNA fragment species having different sequences and being originated from a plurality of DNA sample species, by polymerase chain reaction (PCR), by using a plurality of specific primers and a free primer, wherein an oligonucleotide is introduced by ligation into the 3′-end of each of said target DNA fragment species, said oligonucleotide has a common sequence at the side of the 3′-end of said oligonucleotide and a discriminating sequence which discriminates said target DNA fragment species and follows the 5′-end of said common sequence, said common sequence is common to all the target DNA fragment species and is the same irrespective the length of said target DNA fragment species and the DNA sample species, the length of said discriminating sequence depends on the DNA sample species, the length of said discriminating sequence is the same for said target DNA fragment species originated from the same DNA sample species and different for said target DNA fragment species originated from different DNA sample species, and said target DNA fragment species originated from the different DNA sample species are discriminated by the length of said discriminating sequence, each of said specific primers has a sequence complementary to any said target DNA fragment species to be amplified, said specific primers are immobilized on the surfaces of a plurality of supports separable each other so as to be separated on the base of the kinds of said specific primers, said free primer is contained in a polymcrase chain reaction solution and hybridizes with said common sequence of said oligonucleotide, and recovering the PCR amplification products on the surfaces of said supports, on the basis of the kind of target fragment species.