Patent ID: 7166441
Filing Date: 2007-01-23
Classification: G01N

Abstract:
1. A method for identifying or quantifying polypeptides from a protein sample, comprising: (a) separating polypeptides from said protein sample to provide a support containing said polypeptides; (b) providing at least one digestion membrane adjacent to said support and on which is immobilized at least one polypeptide cleavage reagent in an amount effective for digesting substantially all of the polypeptides in said sample after a single pass of said polypeptides through said membrane; (c) providing at least one capture membrane, downstream, in the direction of migration of peptide fragments of said polypeptides during electroblotting, from said at least one digestion membrane; (d) applying prior to step (e) a MALDI matrix material to the capture membrane; (e) electroblotting said polypeptides from said support through said at least one digestion membrane; (f) collecting said fragments on said at least one capture membrane; and (g) identifying or quantifying said collected fragments by MALDI-TOF mass spectrometry.