Patent ID: 9023769
Filing Date: 2015-05-05
Classification: B01J,C12N,C12Q,C40B

Abstract:
1. A method for preparing a library of DNA copies of a plurality of RNA molecules, comprising: (a) fragmenting the plurality of RNA molecules to produce RNA fragments having a 5′ end and a 3′ end; (b) producing RNA constructs by adding a 5′-RNA oligonucleotide adaptor segment so that it becomes ligated to the 5′ end of the RNA fragments and adding a 3′-RNA oligonucleotide adaptor segment so that it becomes ligated to the 3′ end of the RNA fragments; (c) preparing double-stranded DNA copies of the RNA constructs; and (d) producing a library of circularized double-stranded DNA copies of said RNA fragments, wherein the circularized DNA copies comprise an adaptor that contains a complete DNA copy of the entire 5′-RNA oligonucleotide adaptor segment joined to the entire 3′-RNA oligonucleotide adaptor segment.