Patent ID: 6331416
Filing Date: 2001-12-18
Classification: C07K,C12N

Abstract:
A process of producing a protein of interest in a plant, plant derived tissue or cultured plant cells and of isolating the protein from the plant, plant derived tissue or cultured plant cells, the process comprising the steps of:(a) providing a plant, a plant derived tissue or cultured plant cells expressing a fusion protein including the protein of interest and a cellulose binding peptide being fused thereto, said fusion protein being compartmentalized within cells of said plant, plant derived tissue or cultured plant cells, so as to be sequestered from cell walls of said cells of said plant, plant derived tissue or cultured plant cells;(b) homogenizing said plant, plant derived tissue or cultured plant cells, so as to bring into contact said fusion protein with a plant derived cellulosic matter of said plant, plant derived tissue or cultured plant cells, to thereby effect affinity binding of said fusion protein via said cellulose binding peptide to said cellulosic matter, thereby obtaining a fusion protein cellulosie matter complex; and(c) isolating said fusion protein cellulosic matter complex.