Patent ID: 6368815
Filing Date: 2002-04-09
Classification: C12Q,G01N

Abstract:
An assay for the screening of a candidate molecule that inhibits the expression or the activity of the human phosphodiesterase 4A (PDE 4A) comprising the steps of:a) incubating non-recombinant cells in the presence of a concentration of a phorbol ester sufficient to significantly increase PDE4 production in said cells; b) adding a candidate molecule to the culture medium in which the phorbol ester-treated cells are cultured; c) harvesting and disrupting the cells to produce a cell lysate; d) quantifying cyclic-3â€²,5â€²-adenosine monophosphate (cAMP) content in the cell lysate; and e) comparing the cAMP content measured in step d) with the cAMP content in the cell lysate of the phorbol ester treated cells cultured in the absence of the candidate molecule.