Patent ID: 8772022
Filing Date: 2014-07-08
Classification: A61K,C07K,C12N

Abstract:
1. A chimeric nucleic acid molecule comprising the structural genes Core, E1, and E2, the p7 gene, and the non-structural gene NS2 of any one of a genotype 1a, 1b, 2a, 2b, 3a, 4a, 5a, 6a, or 7a Hepatitis C virus (HCV) and the non-structural genes NS3, NS4A, NS4B, NS5A and NS5B from the HCV genotype 2a JFH1 strain, wherein a reporter gene is inserted in domain III of the non-structural gene NS5A, wherein said NS5A gene encodes a non-structural NS5A protein having a 40 amino acid deletion of amino acid positions 250 to 289 or having a 25 amino acid deletion of amino acid positions 266-290, and wherein said nucleic acid encodes an intragenotypic or intergenotypic recombinant HCV genome that is infective and genetically stable in cell cultures.