Patent ID: 8308924
Filing Date: 2012-11-13
Classification: C12Q,C25D

Abstract:
1. A method for producing an enzyme electrode, comprising: providing a substrate with a carbon surface; forming a gold surface on the carbon surface and thus forming an electrode; modifying the electrode by an amino acid including an amine group, a carboxyl group, and a thiol group in a side chain, wherein the amino acid binds to the gold surface through the thiol group; covalently bonding a mediator containing an aldehyde group to the electrode, wherein the aldehyde group of the mediator and the amine group of the amino acid form a Schiff base; modifying the electrode by a peptide-coupling reagent with a diimide structure, wherein the central carbon atom of the peptide-coupling reagent binds to the carboxyl group of the amino acid through a nucleophilic reaction and thus form an O-acylisourea intermediate; and covalently bonding a glucose oxidase to the electrode, wherein one of the amine groups of the glucose oxidase binds the carboxyl group of the amino acid to form an amide bond by substituting the O-acylisourea intermediate through a nucleophilic substitution reaction.