Patent ID: 7790398
Filing Date: 2010-09-07
Classification: G01N

Abstract:
1. A method of determining the concentration of biologically active human parathyroid hormone in a physiological sample, wherein said method comprises: contacting a first antibody with a sample, wherein said sample comprises a body fluid, wherein the first antibody specifically recognizes human parathyroid hormone polypeptide chains and fragments thereof which comprise oxidized residues at methionine 8 and/or methionine 18; contacting a second antibody with the sample, wherein said second antibody specifically recognizes an epitope formed by the N-terminal amino acids 1 to 3 of the human parathyroid hormone polypeptide chains; and contacting a third antibody with the sample, where the third antibody specifically recognizes an epitope within amino acids 15 to 22 of the human parathyroid hormone polypeptide chains, whereby human parathyroid hormone comprising oxidized methionine residues are precluded from detection by binding of the first antibody, which blocks the second antibody and/or third antibody from binding to oxidized parathyroid hormone polypeptide chains and fragments thereof, thereby determining the concentration of biologically active human parathyroid hormone recognized by the second and third antibodies.