Patent ID: 9080183
Filing Date: 2015-07-14
Classification: C07K,C12N

Abstract:
1. A method for the selection of a cell expressing a heterologous polypeptide to the cell expressing it comprising the following steps: a) transfecting an isolated eukaryotic cell with a nucleic acid comprising i) a first expression cassette comprising a nucleic acid encoding a heterologous polypeptide, ii) a second expression cassette comprising a first nucleic acid comprising the sequence of SEQ ID NO: 04 and a second nucleic acid encoding a selectable marker selected from the group consisting of hygromycin phosphotransferase, neomycin and G418 aminoglycoside phosphotransferase, dLNGFR and GFP, whereby said first and second nucleic acid are operably linked, b) cultivating said transfected cell under conditions suitable for the growth of non-transfected cell; and c) cultivating said cells under selective culture conditions; d) selecting a cell propagating in step b) and under selective culture conditions in step c).