Patent ID: 9188596
Filing Date: 2015-11-17
Classification: G01N,Y10T

Abstract:
1. A method for quantitating vitamin D analytes in a plasma or serum sample, the vitamin D analytes comprising one or more of vitamin D2, vitamin D3, metabolites of vitamin D2, and metabolites of vitamin D3, the method comprising: subjecting the sample containing vitamin D analytes to a two stage purification in which an internal dihydroxy Vitamin D eluting material from the diatomaceous earth or celite column with diisopropyl ether into a silica column; eluting material from the silica column with isopropanol in hexanes; derivatizing the material eluted from the silica column with a QAO-Cookson reagent and subjecting the derivatized material to a liquid chromatography step and performing mass spectrometry analysis on the eluent from the liquid chromatography step using parent daughter ion transition monitoring.