Patent ID: 6037526
Filing Date: 2000-03-14
Classification: A01H,C12N

Abstract:
A transgenic monocotyledonous plant of the Gramineae family obtained by a method for transforming plants with cloned viral DNA, wherein said cloned viral DNA, normally not infectious upon mechanical inoculation, is amenable to transformation by a transfer microorganism of the genus Agrobacterium, said method comprising:(a) inserting cloned viral DNA capable of giving rise to a systemic infection and that may contain cargo DNA, into a T-replicon of an Agrobacterium, having one or more T-DNA border sequences, wherein the distance between said cloned viral DNA and the T-DNA border sequences is chosen such that cloned viral DNA, including any cargo DNA present, is genetically transferred to the plant material;(b) introducing the T-replicon into a transfer microorganism of the genus Agrobacterium, the replicon passing into the transfer microorganism;(c) preparing a microorganism-containing transforming suspension culture comprising the transfer microorganism obtained in step (b); and(d) infecting plant material with the transfer microorganism that has been modified in accordance with step (b),said transgenic plant comprising in an expressible form a chimaeric DNA construct comprising an expressible DNA in operable linkage with expression signals active in plant cells of graminaceous monocots.