Patent ID: 8697364
Filing Date: 2014-04-15
Classification: C12N,C12P

Abstract:
1. A high throughput method for transfection of filamentous fungal cells, comprising: (a) providing a multitude of containers; (b) filling into each container an amount of polymer needed for the transfection, wherein said polymer comprises DNA, RNA, and/or protein; (c) filling cells to be transfected as well as an aqueous solution of transfection reagent, containing not more than 30% of polyethylene glycol into each of the containers; (d) incubating the resulting mixture; (e) optionally removing the transfection reagent from the incubated mixture; and (f) selecting the cells which have been transformed, wherein the total volume of the incubating mixture is less than 1 ml per container; wherein the cells are sorted prior to transfection based on at least one of nuclear content, cell size, cell integrity, cell composition, cell viability, and staining methods.