Patent ID: 7723111
Filing Date: 2010-05-25
Classification: A61K,C12N

Abstract:
1. A method of preparing dual specificity T lymphocytes comprising: (i) contacting a population of T lymphocytes ex vivo with an allogeneic cell selected from the group consisting of dendritic cells, B lymphocytes and peripheral blood mononuclear cells (PBMCs), wherein contacting T lymphocytes with the allogeneic cell selects and specifically amplifies T lymphocytes comprising a T cell receptor that is reactive with the allogeneic cell; and (ii) transducing the T lymphocytes comprising the T cell receptor reactive with the allogeneic cell with a chimeric receptor gene, said gene encoding a chimeric fusion receptor between a single chain antibody that recognizes a tumor antigen and a T cell receptor capable of triggering T cell receptor signal transduction, which is reactive with a tumor antigen, to produce dual specificity T lymphocytes, wherein the dual specificity T lymphocytes generate an anti-tumor immune response in vivo.