Patent ID: 7531179
Filing Date: 2009-05-12
Classification: A61K,A61P,C07K,G01N,Y10S

Abstract:
1. A tolerogizing composition capable of inducing a specific immunological tolerance to a botulinum toxin antigen, said tolerogizing composition comprising a tolerogizing agent conjugated to a BoNT/A peptide; wherein said BoNT/A peptide is at most 60 amino acids in length and comprises amino acids 771-789 of SEQ ID NO: 1, amino acids 785-803 of SEQ ID NO: 1, a conservative BoNT/A amino acid sequence variant thereof, or a tolerogenic BoNT/A amino acid sequence fragment thereof; wherein said amino acids 771-789 of SEQ ID NO: 1, amino acids 785-803 of SEQ ID NO: 1, a conservative BoNT/A amino acid sequence variant thereof and a tolerogenic BoNT/A amino acid sequence fragment thereof produce a decrease in a humoral immunological response to a botulinum toxin antigen thereby inducing a specific immunolocical tolerance to the botulinum toxin antigen; wherein said conservative BoNT/A amino acid sequence variant producing a decreased immunological response comprises 1-4 conservative amino acid substitutions to amino acids 771-789 of SEQ ID NO: 1, or 1-4 conservative amino acid substitutions to amino acids 785-803 of SEQ ID NO: 1; and wherein said tolerogenic BoNT/A amino acid sequence fragment producing a decreased immunological response comprises at least six consecutive amino acids of 771-789 of SEQ ID NO: 1, or at least six consecutive amino acids of 785-803 of SEQ ID NO: 1.