Patent ID: 6617494
Filing Date: 2003-09-09
Classification: A01H,C07K,C12N,Y02A

Abstract:
A method of determining the successful transformation of an Arabidopsis plant with a heterologous polynucleotide, comprising:transforming an Arabidopsis plant with a DNA construct comprising at least one heterologous polynucleotide operatively associated with a promoter capable of regulating transcription in a plant and a wild-type GL1 encoding polynucleotide expressed from its own promoter, wherein the plant is homozygous for a mutation of the GL1 gene; collecting seeds from the plant and growing the seeds into progeny plants; and detecting the presence or absence of trichomes on the progeny plants, wherein the presence of trichomes indicate the successful transformation of the progeny plants with the heterologous polynucleotide.