Patent ID: 9029148
Filing Date: 2015-05-12
Classification: C12N

Abstract:
1. A process for the preparation of heterogenic fibroblasts comprising the steps of: a) Providing a cell-containing tissue sample; b) Preparing a suspension of primary cells comprising tissue pieces from the cell-containing tissue sample in a medium; c) Culturing the suspension of primary cells comprising tissue pieces in a vessel coated with a matrix wherein a batch of fibroblast cell nests is generated on the matrix of the vessel; d) Separating the fibroblast cell nests of step c) from the suspension of primary cells comprising tissue pieces; e) Repeating steps c) to d) at least once whereas the suspension of primary cells comprising tissue pieces of step d) is re-used within step c) to generate at least one more batch of fibroblast cell nests; wherein the at least two batches of fibroblast, cell nests obtained through steps c) to e) have different types of fibroblasts, and wherein a combination of the at least two batches of fibroblast cell nests provides heterogenic fibroblasts.