Patent ID: 7384923
Filing Date: 2008-06-10
Classification: A61K,C12N

Abstract:
1. A method to generate both a cell-based and humoral immune response to a target polypeptide in an animal, which method comprises administering subcutaneously or intramuscularly to the animal a composition comprising liposomes suspended in an aqueous liquid and a polynucleotide comprising a promoter operatively linked to a nucleotide sequence encoding said target polypeptide, wherein the liposomes comprise phosphatidylcholine (PC), dioleoyl phosphatidyl ethanolamine (DOPE), and 1,2-dioleoyloxy-3-trimethyl ammonium propane (DOTAP), have diameters in the range 100 to 2000 nm and comprise a lipid bilayer and an aqueous intravesicular space, wherein said polynucleotide is entrapped in the aqueous intravesicular space, wherein said lipid bilayer includes said DOTAP in an amount such that the lipid bilayer has an overall cationic charge; whereby said polynucleotide is delivered to and is expressed in target cells whereby an immune response including an IgG response and Th1 and Th2 responses to the target polypeptide result; wherein said polynucleotide is administered in an amount sufficient to elicit said immune response.