Patent ID: 7407812
Filing Date: 2008-08-05
Classification: C07K,Y10T

Abstract:
1. Method for the enrichment of phosphopeptides, comprising of the following method steps: a) provision of a support material having chelate ligands based on silica b) activation of the support material from step a) using transition-metal ions, oxides or oxide hydrates of transition-metal ions or trivalent ions of metals from the third main group c) bringing a phosphopeptide-containing sample into contact with the activated support material in the presence of a binding buffer d) removal of the supernatant consisting of the binding buffer and the unbound part of the sample e) optionally washing of the support material f) elution of the phosphopeptides with an elution buffer which has a pH>10 and comprises alkali metal, alkaline earth metal or ammonium salts of thiocyanate, of acids of the complex ligands nitrito, isocyano, nitrile, isocyanato, isothiocyanato, azido, ethylenediamine, isonitrile, fulminato and cyano and/or of the oxygen acids of phosphorus, sulfur, vanadium, ruthenium, niobium, tantalum, tungsten or of molybdenum, and/or chelating agents.