Patent ID: 7476511
Filing Date: 2009-01-13
Classification: G01N

Abstract:
1. A method for detecting a mimic of paclitaxel in a paclitaxel binding site of a microtubule, comprising: (a) providing a target microtubule and a probe wherein the target microtubule is assembled in vitro and stabilized by means of chemical cross-linking and wherein the target microtubule is indefinitely conserved in liquid nitrogen following dialysis against a conservation and cryopreservation buffer, (b) adding a test substance to a solution of a target microtubule consisting of said target microtubule and a fluorescent probe bound to the target microtubule, (c) determining the drop in anisotropy of said solution at varying test substance concentrations, and (d) identifying the test substance as a paclitaxel mimic by means of such drop in fluorescence anisotropy or by measuring the resonance energy transfer to the probe to a suitable acceptor.