Patent ID: 8852934
Filing Date: 2014-10-07
Classification: C12N

Abstract:
1. A method of producing insulin-producing tissues (IPTs) by culturing, comprising: (a) seeding pancreatic discards, which are left-over tissues remaining after isolation of islets from a postnatal pancreas, in a first culture container or culture substrate to produce floating tissue debris and non-endocrinal epithelial cells (NEECs), wherein said NEECs adhere to the culture container or culture substrate; (b) separating said NEECs from said floating tissue debris; (c) culturing said separated NEECs in vitro in a first culture medium and isolating said NEECs; (d) seeding said separated floating tissue debris into a second culture medium to produce vascular endothelial cells (VECs) and second floating tissue debris; (e) separating said vascular endothelial cells (VECs) from said second floating tissue debris; (f) culturing said separated VECs in vitro in a third culture medium and isolating said VECs; (g) mixing said isolated NEECs and said isolated VECs; and (h) generating, in vitro, a tissue complex comprising the mixture of said isolated NEECs and said isolated VECs.