Patent ID: 6232085
Filing Date: 2001-05-15
Classification: B01L,C07K,C12Q,C40B,G01N,Y02A

Abstract:
A multi-variable methodforranking theefficacyofoneormore of a multiplicity of different biochemical conditions for stabilizing a target molecule which is capable of unfolding due to a thermal change, which comprises(a) contacting said target molecule with one or more of said multiplicity of different biochemical conditions in each of a multiplicity of wells in a microplate;(b) simultaneously heating said multiplicity of wells from step (a);(c) measuring in each of said wells a physical change associated with the thermal unfolding of said target molecule resulting from said heating;(d) generating a thermal unfolding curve for said target molecule as et function of temperature for each of said wells;(e) comparing each of said unfolding curves in step (d) to (i) each of said other unfolding curves and to (ii) the unfolding curve obtained for said target molecule under a reference set of biochemical conditions; and(f) ranking the efficacies of said multiplicity of different biochemical conditions for stabilizing said target molecule against heat-induced unfolding according to the change in each of said thermal unfolding curves.