Patent ID: 9051357
Filing Date: 2015-06-09
Classification: C07K

Abstract:
1. A fibronectin monobody polypeptide comprising a first fibronectin type III (Fn3) monobody fragment having at least two Fn3 beta-strand domains with a loop region linked between each beta-strand domain, and at least one additional fibronectin monobody fragment associated with the first Fn3 monobody fragment, comprising at least two fibronectin beta strand domains with a loop region linked between each beta-strand domain, wherein the loop regions of the first Fn3 monobody fragment and the at least one additional fibronectin monobody fragment correspond to wild-type fibronectin loop regions, or comprise an AB, BC, CD, DE, EF or FG loop region sequence that is randomized or varies from a corresponding wild-type fibronectin loop region by deletion of one to all except one amino acid in the loop region, insertion of one to 25 amino acids, and/or replacement of at least one amino acid in the loop region, wherein at least one loop region from the first fibronectin type III (Fn3) monobody fragment or the at least one additional fibronectin monobody fragment is capable of binding to a specific binding partner (SBP) to form a polypeptide:SBP complex.