Patent ID: 8124399
Filing Date: 2012-02-28
Classification: C07K,C12N,C12P,C12R,C12Y

Abstract:
1. An altered Bacillus strain comprising a heterologous protein of interest and a deletion of one or more indigenous chromosomal regions selected from the group consisting of a PBSX region corresponding to about 1319663 to 1348691 bp, a skin region corresponding to about 2653562 to 2699604 bp, a prophage 7 region corresponding to about 2701087 to 2749642 bp, a prophage 1 region corresponding to about 202112 to 220141 bp, a prophage 2 region corresponding to about 529067 to 569578 bp, a prophage 3 region corresponding to about 652000 to 664300 bp, a prophage 4 region corresponding to about 1262987 to 1313692 bp, a prophage 5 region corresponding to about 1879200 to 1900000 bp, a prophage 6 region corresponding to about 2046050 to 2078000 bp, a PPS region corresponding to about 1960409 to 1998026 bp, a PKS region corresponding to about 1781795 to 1857985 bp, a YVFF-YVEK region corresponding to about 3513137 to 3528896 bp, a DHB region corresponding to about 3279418 to 3292920 bp, or a fragment thereof, wherein the coordinates correspond to the B. subtilis strain 168 chromosome, and wherein said altered Bacillus strain has an enhanced level of expression of the heterologous protein of interest compared to a corresponding unaltered Bacillus strain when said altered and unaltered Bacillus strains are grown under essentially the same growth conditions.