Patent ID: 9029635
Filing Date: 2015-05-12
Classification: C12N,Y02A

Abstract:
1. A method to form a sucrose storage organ of a sugar beet or a sugarcane in which the sucrose concentration is increased relative to the sucrose concentration of an unchanged control sucrose storage organ of the same genotype at the stage of harvest, the method comprising a) introducing a nucleic acid sequence comprising SEQ ID NO: 2, or a nucleic acid sequence able to hybridize under stringent conditions with a sequence complementary to the sequence SEQ ID NO: 2 in a sugar beet or sugar cane cell, said nucleic acid encoding a polypeptide for reducing the enzymatic activity of an invertase, wherein said stringent conditions are hybridization in 4×SSC at 65° C. followed by repeated washing in 0.1×SSC at 65° C. for a total of about one hour, b) regenerating a primary transformant sugar beet or sugar cane plant comprising said nucleic acid from the transformed cell of step a); c) growing said primary transformant plant to produce transgenic seed; d) cultivating said transgenic seed to produce a progeny plant, whereby a sucrose storage organ of said progeny plant is formed in which the sucrose concentration, compared with the sucrose concentration of a non-genetically-modified control sucrose storage organ of the same genotype, is increased at harvest of said sucrose storage organs and non-genetically-modified storage organs, and wherein the sucrose concentration of said storage organ is not negatively correlated with the sucrose storage organ yield, or the increase in sucrose concentration by X percentage points leads to a reduced sucrose storage organ yield and the reduction of the sucrose storage organ yield is a maximum of 5 X percent.