Patent ID: 7915001
Filing Date: 2011-03-29
Classification: C07K,G01N,Y10S,Y10T

Abstract:
1. A method for detecting nitrated fragments of type II collagen by immunoassay, comprising the steps of: contacting a biological sample with an antibody that specifically binds the nitrated epitope LQY-NO 2 -MRA (SEQ ID NO: 7), wherein the amino acid residue Y—NO 2 therein is a nitrated tyrosine residue, and detecting binding of the antibody to fragments of type II collagen in the sample that comprise said nitrated epitope; wherein said antibody specifically binds said nitrated tyrosine residue in conjunction with the specific amino acid sequence of said nitrated epitope, and wherein said antibody does not react with the non-nitrated sequence LQYMRA (SEQ ID NO: 2).