Patent ID: 6613895
Filing Date: 2003-09-02
Classification: C12N

Abstract:
A method for isolating nucleic acid from a sample containing nucleic acid, which method comprises:a) forming a binding reaction admixture by admixing said sample with an insoluble silica matrix and an aqueous salt solution, said insoluble silica matrix comprising particulate glass having a sedimentation time through 100 centimeters (cm) of still water at unit gravity in the range of 6 weeks to 20 minutes, said solution containing a salt at a concentration of at least 3 molar; b) maintaining said binding reaction admixture for a time period sufficient for said nucleic acid to bind to said matrix to form an insoluble nucleic acid-matrix complex and a remaining admixture; c) separating said remaining admixture and said complex to form an isolated complex; and d) recovering said nucleic acid from said isolated complex to form isolated nucleic acid.