Patent ID: 8530221
Filing Date: 2013-09-10
Classification: C12N,C12P

Abstract:
1. A recombinant microbial cell comprising: (a) polynucleotides encoding a branched chain alpha-keto acid dehydrogenase (BKD) complex which comprises polypeptides having branched-chain alpha-keto acid dehydrogenase activity, lipoamide acyltransferase activity, and dihydrolipoamide dehydrogenase activity, (b) a polynucleotide encoding a polypeptide having beta-ketoacyl-ACP synthase activity that utilizes a branched acyl-CoA molecule as a substrate, (c) one or more polynucleotides encoding a polypeptide having fatty acid derivative enzyme activity, wherein the fatty acid derivative enzyme activity comprises one or more of thioesterase activity, fatty alcohol biosynthetic activity, ester synthase activity and hydrocarbon biosynthetic activity, and (d) one or more polynucleotides encoding a polypeptide having aspartokinase activity, homoserine dehydrogenase activity, homoserine kinase activity, threonine synthase activity or threonine deaminase activity, wherein at least one polynucleotide according to (a), (b) or (d), encodes a polypeptide that is exogenous to the recombinant microbial cell or wherein expression of at least one polynucleotide according to (a), (b) or (d), is modulated in the recombinant microbial cell as compared to the expression in a corresponding wild-type microbial cell, and wherein the recombinant microbial cell produces a branched fatty acid derivative when cultured in the presence of a carbon source under conditions effective to express the polynucleotides.