Patent ID: 6284481
Filing Date: 2001-09-04
Classification: C12Q,G01N,Y02A

Abstract:
A spectrophotometric assay for detecting hemoglobin degradation which comprises:a) preparing a mixture comprising hemoglobin and a proenzyme of a hemoglobin-degrading enzyme, wherein the mixture has a pH in the range from 7.5 to 7.6,b) measuring the absorbance of the mixture at a wavelength (.lambda.) in the range from 404 to 407 nm,c) acidifying the mixture to activate the hemoglobin-degrading enzyme,d) incubating the mixture of step c),e) measuring the absorbance of the mixture of step d) at a wavelength (.lambda.) in the range from 404 to 407 nm,f) adding a base to the mixture of step e) to effect renaturation of undegraded hemoglobin,g) incubating the mixture of step f), andh) measuring the absorbance of the mixture of step g) at a wavelength (.lambda.) in the range from 404 to 407 nm.