Patent ID: 8835361
Filing Date: 2014-09-16
Classification: G01N

Abstract:
1. A method for quantitating plant allergen in a target plant seed, comprising: (a) obtaining a protein sample from said target plant seed; (b) analyzing said protein sample using mass spectrometry with a set of pre-designed internal standard peptides, wherein said peptides are specific to said plant allergen; and (c) monitoring frequencies and intensities of resulting spectra to obtain relative and/or absolute allergen contents in said target plant seed; wherein the pre-designed internal standard peptides have a decreased content of cysteines, methionines, tryptophans, and histidines relative to other peptides of the same length specific to said plant allergen; and wherein the pre-designed internal standard peptides have a decreased content of internal and adjacent chemical or enzymatic cleavage sites relative to other peptides of the same length specific to said plant allergen.