Patent ID: 9211306
Filing Date: 2015-12-15
Classification: A61K,A61P,C12N

Abstract:
1. A method for treating urinary incontinence in a patient in need thereof, said method comprising: (i) obtaining tissue from the decidua of the placenta or the decidua of menstrual fluid and finely cutting the tissue; (ii) digesting the finely cut tissue in medium containing collagenase, and then removing non-digested tissue; (iii) centrifuging the digested tissues and then suspending the resulting pellets to obtain single cells; (iv) culturing the single cells in a culture vessel in a medium containing bFGF, whereby stem cells attach to the culture vessel; (v) collecting the attached stem cells; (vi) formulating a cellular therapeutic agent comprising the stem cells; and (vii) injecting the cellular therapeutic agent into the patient's lower abdominal region, thereby increasing leak-point pressure, wherein said stem cells have the following characteristics: (a) positive for CD29 and CD90, but negative for CD31 and CD45; (b) positive for Oct4, SSEA-4 and Cripto-1; (c) ability to grow attached to plastic, showing morphological features of round or spindle shape, and forming spheres in serum-free medium so as to be able to be maintained in an undifferentiated state; and (d) having the ability to differentiate into myocytes.