Patent ID: 7696491
Filing Date: 2010-04-13
Classification: G02B

Abstract:
1. A fluorescence observation or measurement system for observing or measuring a specimen that uses a living cell and emits fluorescent light, the fluorescence observation or measurement system comprising: an objective lens including optical elements made of glass; an immersion substance; and a cover glass, wherein at least one of the following Conditions (1a-1),(1b-1) and (1c-1) is satisfied: wherein the specimen satisfies at least one of the following Conditions (2-1) and (3-1): where B OB ′ is an average intensity value of auto-fluorescence from the objective lens, B OB is an average intensity value of auto-fluorescence from a conventional objective lens generally used, B IM ′ is an average intensity value of auto-fluorescence from the immersion substance, B IM is an average intensity value of auto-fluorescence from a conventional immersion substance generally used, B CG ′ is an average intensity value of auto-fluorescence from the cover glass, B CG is an average intensity value of auto-fluorescence from a conventional cover glass generally used, S is an average intensity value of fluorescent light emanating from the specimen, s is a fluctuation range of the intensity of the fluorescent light, B is an average intensity value of a background noise from where an object to be observed or measured is absent in the specimen, and b is a fluctuation range of the intensity of the back ground noise.