Patent ID: 6489541
Filing Date: 2002-12-03
Classification: C12N

Abstract:
A method of using a DNA molecule comprising a nucleotide sequence encoding IAGlu Transferase to produce a transgenic plant, said method comprising the steps ofa) cloning a nucleotide sequence encoding IAGlu Transferase having an amino acid sequence as given in SEQ ID NO:2 operably linked to transcriptional control sequences comprisng a promoter functional in a plant cell to produce an IAGlu Transferase expression construct; b) Cloning the IAGlu Transferase expression construct of step (a) into a plasmid vector adapted for use in a plant cell to produce a plant IAGlu Transferase expression vector; c) introducing said plant IAGlu Transferase expression vector of step (b) into plant tissue to produce transgenic plant tissue; and d) regenerating said transgenic plant tissue to produce a transgenic plant, whereby IAGlu Transferase is expressed at greater than natural levels in at least one tissue of said transgenic plant, with at least one effect selected from a group consisting of inhibition of apical dominance, inhibition of stem elongation, inhibition of cell enlargement, and increased numbers of stems per plant as compared with a wild-type of said plant.