Patent ID: 7482151
Filing Date: 2009-01-27
Classification: C02F,C07K,C12N,C12P,C12Q,C12R,Y02W

Abstract:
1. A method of alleviating or preventing the accumulation of ammonia in an aquarium comprising: providing a bacterial strain that oxidizes ammonia to nitrite, wherein said bacterial strain comprises a nucleotide sequence that has at least 96% identity over the full length thereof to SEQ ID NO:1; and introducing into the aquarium and growing an amount of the bacterial strain sufficient to alleviate or prevent the accumulation of ammonia in the aquarium, wherein hybridization to SEQ ID NO:1 occurs under the following stringent conditions: hybridization in 30% formamide, 0.9 M NaCl, 0.01% sodium dodecyl sulphate, 20 mM Tris/HCl pH 7.4 at 46° C., and washing with 120 mM NaCl, 5 mM EDTA, 0.01% sodium dodecyl sulphate, and 20 mM Tris/HCl at 48° C.