Patent ID: 7853408
Filing Date: 2010-12-14
Classification: G16B

Abstract:
1. Method for the design of oligonucleotides that can be used in molecular biology procedures, for identification of a metagenomic sample wherein it comprises the steps of (a) building a reference sequence database from a metagenomic sample; (b) selecting a subset of sequences (target sequences) corresponding to target organisms, according to taxonomic classification to build a design database; (c) selecting candidate oligonucleotides from the target sequences in the design database, by considering all subsequences of a defined length, between 18 and 50 nucleotides, present in the target sequences and their reverse-complementary sequences; (d) depurating these candidate oligonucleotides according to hybridization specificity and thermodynamic stability criteria, wherein the thermodynamic stability criteria are: (e) obtaining the list of oligonucleotides that fulfill requirements of step (d); (f) producing materially, by chemical synthesis, the designed oligonucleotides; and (g) selecting the oligonucleotides which comply with the requirements of the desired process.