Patent ID: 6946153
Filing Date: 2005-09-20
Classification: A61K,Y10S

Abstract:
1. A method of treating a patient suffering a disease associated with Trichophyton mentagrophytes or Pityrosporum ovale by applying topically an anti-fungal pharmaceutical composition comprising a therapeutically effective amount of a product prepared from rhizomes of Zingiber officinale , as an active ingredient, in admixture with a pharmaceutically acceptable carrier or diluent for the active ingredient, wherein said product is prepared by the following steps: a) preparing a crude liquid from rhizomes of b) introducing the crude liquid to a reverse phase chromatography column, and eluting the column with water, a first eluent and a second eluent in sequence, said second eluent having a polarity weaker than that of the first eluent but stronger than that of chloroform, so that a first eluent resulting from elution of the first eluent and a second eluate resulting from elution of the second eluent are obtained; c) removing the first eluent from the first eluate by evaporation, so that a first concentrated eluate is obtained and is able to be used as the product; and d) removing the second eluent from the second eluate by evaporation, so that a second concentrated eluate is obtained and is able to used as the product; wherein step a) comprises steps i) to iv), or comprises step I), step I′), or step I″), wherein said steps i) to iv) are: i) shedding fresh rhizomes of ii) extracting the filtrate with a first organic solvent, recovering the resulting extraction solution of the first organic solvent, and evaporating the first organic solvent from the extraction solution to obtain a first concentrated extraction solution; iii) extracting the residue with a second organic solvent, recovering the resulting extraction solution of the second organic solvent, and evaporating the second organic solvent from the extraction solution to obtain a second concentrated extraction solution; and iv) combining the first concentrated extraction solution and the second concentrated extraction solution to obtain the crude liquid; said step I) is: I) extracting powder of dried rhizomes of said step I′) is: I′) steam distilling powder of dried rhizomes of said step I″) is: I″) extracting powder of dried rhizomes of