Patent ID: 6300065
Filing Date: 2001-10-09
Classification: C12N,C40B,G01N

Abstract:
A method for selecting proteins with enhanced phenotypic properties relative to those of a wild-type of said protein, comprising the steps of:transforming yeast cells with a vector expressing a protein to be tested fused to a yeast cell wall protein, wherein mutagenesis is used to a generate a variegated population of mutants of the protein to be tested;contacting said yeast cells with a first label, wherein said first label associates with yeast expressing said protein having enhanced phenotypic properties and does not associate with yeast which do not express said protein having enhanced phenotypic properties;isolating said yeast cells with which said first label is associated;analyzing and comparing said phenotypic properties of said mutant protein expressed by yeast with phenotypic properties of said wild-type protein; andselecting yeast cells exhibiting mutant proteins with enhanced phenotypic properties over the wild-type protein;where enhanced phenotypic properties are one or more of surface expression level, stability, secretion levels and solubility.