Patent ID: 8940504
Filing Date: 2015-01-27
Classification: C07K,C12N,C12Y

Abstract:
1. A method for producing a composition comprising a Vitamin K-dependent protein of interest having a substantially lower amount of protein contaminant Protein S expressed endogenously by a Chinese Ovary Hamster (CHO) host cell in the absence of modification, said method comprising the steps of: a) producing said host cell expressing a Vitamin K-dependent protein of interest comprising the following steps in any order: b) growing said host cell in a growth medium and harvesting said growth medium comprising said Vitamin K-dependent protein of interest; wherein said Vitamin K-dependent protein of interest is selected from the group consisting of: Protein S, GAS-6, Factor II (Prothrombin), Factor X, Factor IX, Protein C, Factor VII, Protein Z, Transmembrane gamma-carboxyglutamic acid protein 1, Transmembrane gamma-carboxyglutamic acid protein 2, Transmembrane gamma carboxyglutamic acid protein 3, Transmembrane gamma-carboxyglutamic acid protein 4, Bone Gla protein, Matrix Gla protein, and Osteocalcin.