Patent ID: 6784981
Filing Date: 2004-08-31
Classification: G01N,H04L

Abstract:
A flow cytometry-based hematology system for classifying and counting biological cells in a blood sample or blood-derived sample, comprising:a hydraulic system including a flow cell, said hydraulic system being capable of mixing said blood sample or blood-derived sample with the contents of one or more of a disposable vessel, a lyse tube or a sheath tube, and being capable of moving the contents of one or more of said disposable vessel, said lyse tube, said sheath tube, or a mixture of said blood sample or said blood-derived sample with one or more of said contents of said disposable vessel, said lyse tube and said sheath tube through said flow cell; a diode laser, said diode laser emitting light to irradiate cells present in said flow cell, said cells being derived from said blood sample or said blood-derived sample; a lensless light detection system, said lensless light detection system having a first portion capable of detecting one or more of axial light loss, low-angle scattered light and high-angle scattered light, and a second portion capable of detecting right angle scattered light scattered at a high numerical aperture, said second portion being physically distinct from said first portion, said first portion and said second portion being capable of converting said axial light loss, said low-angle scattered light, said high-angle scattered light, and said right angle scattered light into electrical signals; a signal processor, said signal processor being capable of analyzing said electrical signals generated from said first portion and said second portion of said lensless light detection system, and generating data therefrom; and a servo circuit capable of correcting for the influence of dark current noise and non-scattered light incident on said lensless light detection system.