Patent ID: 9080178
Filing Date: 2015-07-14
Classification: C12N,C12P,Y02P

Abstract:
1. A transgenic Escherichia coli cell comprising an additional copy of Escherichia coli alanine dehydrogenase (DadA) gene, and a deletion in the endogenous cydA gene (SEQ ID NO: 1), cydB gene (SEQ ID NO: 2), cyoA gene (SEQ ID NO: 3), cyoB gene (SEQ ID NO: 4), cyoC gene (SEQ ID NO: 5), cyoD gene (SEQ ID NO: 6), cbdA gene (SEQ ID NO: 7), cbdB gene (SEQ ID NO: 8), ygiN gene (SEQ ID NO: 9), and ldhA gene, wherein said transgenic Escherichia coli cell a) has substantially the same level of growth in oxic conditions as the level of growth in anoxic conditions of the corresponding b) is capable of converting glucose to D-lactate under oxic conditions and/or anoxic conditions, c) has substantially the same level of growth as said corresponding d) is capable of producing an increased level of alanine compared to the corresponding