Patent ID: 6204326
Filing Date: 2001-03-20
Classification: B01J,C07K

Abstract:
A method of synthesis of a peptide nucleic acid on an activated solid phase synthesis resin comprising the steps of:a) treating said activated solid phase synthesis resin with a monoprotected diamine synthon to form a first synthon bound to said resin and where said first synthon includes as an integral part thereof, a secondary amine and a protected primary amine;b) deprotecting said first synthon to form a second synthon having as an integral part thereof said secondary amine and a free amine;c) treating said second synthon with a 2-haloacetic acid ester to form a third synthon having as an integral part thereof said secondary amine and an acetyl ester;d) treating said third synthon with a 1-haloacetylnucleobase to form a fourth synthon having as an integral part thereof a covalently bound acetylnucleobase and said acetyl ester;e) deprotecting said fourth synthon to form a fifth synthon having as an integral part thereof a free carboxylic acid and said covalently bound acetylnucleobase;f) treating said fifth synthon with a further monoprotected diamine synthon forming an extended first synthon;g) repeating steps f, b, c, d, and e until said peptide nucleic acid is of desired length; andh) terminating said synthesis at step d or e.