Patent ID: 6352835
Filing Date: 2002-03-05
Classification: C12Q,G01N

Abstract:
A method of measuring an analyte in a sample using a redox reaction, comprising:eliminating the influence of any reducing substance contained in the sample prior to conducting the redox reaction, the influence of any reducing substance being eliminated by adding a tetrazolium compound prior to the redox reaction; forming a reducing substance or an oxidizing substance derived from the analyte; measuring the quantity of the formed substance derived from the analyte by the redox reaction; and determining the quantity of the analyte from the quantity of the formed substance derived from the analyte; wherein the tetrazolium compound used to eliminate the influence of any reducing substance comprises at least one of 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt, 2-(4-iodophenyl)-3-(2,4-dinitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt, 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium salt, 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium salt, 3,3â€²-(1,1â€²-biphenyl-4,4â€²-diyl)-bis(2,5-diphenyl)-2H-tetrazolium salt, 3,3â€²-[3,3â€²-dimethoxy-(1,1â€²-biphenyl)-4,4â€²-diyl]-bis[2-(4-nitropenyl)-5-phenyl-2H-tetrazolium salt, 2,3-diphenyl-5-(4-chlorophenyl)tetrazolium salt, 2,5-diphenyl-3-(p-diphenyl)tetrazolium salt, 2,3-diphenyl-5-(p-diphenyl)tetrazolium salt, 2,5-diphenyl-3-(4-styrylphenyl)tetrazolium salt, 2,5-diphenyl-3-(m-tolyl)tetrazolium salt, and 2,5-diphenyl-3-(p-tolyl) tetrazolium salt.