Patent ID: 8105766
Filing Date: 2012-01-31
Classification: C12Q

Abstract:
1. A method of determining the concentration of pyrophosphate in a sample, which method comprises the following steps (1) a step comprising a first reaction for producing ATP by contacting pyrophosphate which may be present in the sample with pyruvate orthophosphate dikinase in the presence of at least AMP; (2) a step comprising a second reaction for producing pyrophosphate and NAD by contacting the ATP produced in the first reaction with nicotinamide-nucleotide adenylyltransferase; (3) a step comprising a third reaction for reducing the NAD produced by the second reaction into NADH; (4) a step comprising detecting the amount of NADH produced by the third reaction; and (5) determining the concentration of pyrophosphate in the sample based on the amount of NADH produced in the above step.