Patent ID: 6790624
Filing Date: 2004-09-14
Classification: C07K,C12N,C40B,G01N

Abstract:
An assay for determining the activity of a proteinâ€”protein interaction comprising a) transforming a cell with a first vector containing a gene encoding a fusion protein comprising a protein containing a modular protein binding domain MPBD and an exogenously introduced a first coiled-coil dimerization which comprises a WIN-ZIP-A1 synthetic amphipathic helix and with a second vector containing a gene encoding a second fusion protein comprising a binding site for the MPBD and an exogenously introduced second coiled-coil dimerization domain interactive with the first dimerization domain, which comprises a WIN-ZIP-B1 synthetic amphipathic helix, b) culturing the transformed cell, c) comparing the activity to a base line control, d) detecting and measuring changes in activity for determining th activity of the proteinâ€”protein interaction, wherein the MPBD domain consists of src homology 3 (SH3) and wherein the protein containing the modular protein binding domain (MPBD), which contains a WIN-ZIP-A1 helix is a tyrosine kinase.