Patent ID: 6020170
Filing Date: 2000-02-01
Classification: A61K,A61P,C07K

Abstract:
A method of preparing a monoclonal antibody against a T cell surface antigen, comprising the steps ofa) injecting a mammal with a cell surface antigen-comprising material, wherein said material is selected from the group consisting of i) whole T cells and ii) a membrane fraction obtained by treating whole T cells;b) isolating a B-cells-containing cell fraction from the spleen of said mammal;c) enriching said cell fraction obtained in step b) in B-cells specific for said cell surface antigen by contacting the cell fraction with carrier-bound material of cells related to said whole cells, said related cells lacking said cell surface antigen, and separating B-cells bound to said carrier-bound material of related cells from the enriched unbound B-cells-containing cell fraction to be used in the next step;d) subjecting the enriched B-cells-containing cell fraction obtained in step c) to limiting dilution followed by clonal expansion;e) selecting a B-cell clone and immortalizing said selected B-cell clone using a small-scale fusion technique;f) selecting and cloning a hybridoma capable of producing antibodies which specifically bind said cell surface antigen; andg) isolating a monoclonal antibody-comprising fraction from supernatant of said hybridoma.