Patent ID: 7504234
Filing Date: 2009-03-17
Classification: C12Q,G01N

Abstract:
1. A method of measuring the concentration of homocysteine hydrolyzed by a S-adenosylhomocysteine hydrolase enzyme, comprising: a) contacting S-adenosylhomocysteine with an S-adenosylhomocysteine hydrolase enzyme, wherein said S-adenosylhomocysteine hydrolase enzyme is b) contacting said homocysteine with said thiol sensitive fluorophore under conditions such that said thiol sensitive fluorophore is covalently conjugated to said homocysteine to form a labeled homocysteine; and c) measuring the level of fluorescence from said labeled homocysteine; wherein said level of fluorescence is correlated with the concentration of said labeled homocysteine.