Patent ID: 7704752
Filing Date: 2010-04-27
Classification: C07F,G01N,Y10S,Y10T

Abstract:
1. A method for detecting the presence or absence of hydrogen peroxide, a salt of hydrogen peroxide or a complex of hydrogen peroxide in a sample comprising: a) reacting the sample with a signalling compound of the formula: wherein Sig comprises a substituted or unsubstituted coumarin or a substituted or unsubstituted benzothiazole, B is a boron atom, wherein B is bonded to a ring carbon of the aromatic ring of said coumarin or benzothiazole, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring, with a source of hydrogen peroxide to produce a detectable product Sig-OH or Sig-O— capable of detection by fluorescence; b) irradiating the detectable product with light of a first wavelength; and c) detecting light emitted as fluorescence from the detectable product at a second wavelength different from the first wavelength to provide an indication of the presence of peroxide, wherein the signalling compound itself does not emit fluorescence at the second wavelength or whose ability to emit fluorescence at the second wavelength differs from that of the detectable compound by at least a factor of 10.