Patent ID: 6455274
Filing Date: 2002-09-24
Classification: C12N

Abstract:
An isolated polynucleotide comprising a nucleic acid sequence selected from the group consisting of:(a) a nucleic acid sequence encoding the polypeptide set forth as amino acid residues 1 to 911 of SEQ ID NO:2; (b) a nucleic acid sequence encoding the polypeptide set forth as amino acid residues 2 to 911 of SEQ ID NO:2; (c) a nucleic acid sequence encoding the polypeptide set forth as amino acid residues 68 to 911 of SEQ ID NO:2; (d) a nucleic acid sequence encoding a fragment of the polypeptide set forth as amino acid residues 1 to 911 of SEQ ID NO:2 wherein said fragment retains DNA ligase activity; (e) a nucleic acid sequence encoding a fragment of the polypeptide set forth as amino acid residues 1 to 911 wherein said polypeptide contains at least one conservative amino acid substitution and further wherein said polypeptide retains DNA ligase activity; (f) a nucleic acid sequence encoding at least 50 contiguous amino acid residues of SEQ ID NO:2; (g) a nucleic acid sequence encoding at least 30 contiguous amino acid residues of SEQ ID NO:2; (h) the nucleic acid sequence of a polynucleotide which hybridizes to a polynucleotide consisting of the complement of the nucleic acid sequence shown as SEQ ID NO:1 following incubation in 0.5 M NaPO4, pH 7.4 and 7% SDS overnight at 65Â° C. and washing twice at 60Â° C. and twice at room temperature with 0.5Ã—SSC and 0.1% SDS; and (i) a nucleic acid sequence complementary to the nucleic acid sequence of (a), (b), (c), (d), (e), (f), (g) or (h).