Patent ID: 9068281
Filing Date: 2015-06-30
Classification: C12M,C40B

Abstract:
1. A microfluidic chip for high throughput miniaturized perfusion-based three-dimensional cell culture, including: a roof, which has an array of cell culture units at a bottom surface; each cell culture unit including a cell culture medium inlet hole through a top surface of the roof; the bottom surface of the roof having a negative pressure source hole fluidly connected to the air chamber is coupled to a negative pressure source that generates negative pressure, wherein the negative pressure source drives a medium flow in a single direction from the cell culture medium tank, through the microchannels, and to the waste medium collection and analysis tank; an intermediate plate having a plurality of connecting holes and a plurality of array holes; a bottom plate detachably coupled to the intermediate plate, wherein the bottom plate has a plurality of cylindrical chambers to matingly insert into the plurality of holes; a waste medium collector tray module detachably coupled to the intermediate plate, wherein the module has an array of cylindrical slots to matingly insert into the plurality of array holes.