Patent ID: 9200989
Filing Date: 2015-12-01
Classification: G01N

Abstract:
1. A method for three-dimensional microdissection, to cut out a defined three-dimensional structure from a sample in the submillimeter range, comprising: providing a device for three-dimensional microdissection, the device comprising at least an ablation chamber and a laser device having an objective lens providing a laser focus, wherein the objective lens of the laser device is introduced into the ablation chamber to cut the sample, generating a three-dimensional volume model that defines the three-dimensional structure; cutting out the three-dimensional structure from the sample by performing at least one of cold laser ablation or multi-photon absorption based on the three-dimensional volume model that is defined by a three-dimensional data field comprising data representing all spatial directions, wherein the cutting out comprises relatively moving the sample with respect to the laser focus to remove layers of the sample in three dimensions by at least one of cold laser ablation or multi-photon absorption to thereby cut out the three-dimensional structure, wherein the three-dimensional structure remains undamaged when the cutting out is completed.