Patent ID: 6017943
Filing Date: 2000-01-25
Classification: A61K,C07K

Abstract:
A process for preparing an intra-crosslinked hemoglobin composition having the formula:.alpha..beta.--S--Y--R--CH.sub.2 --CH.sub.2 --[O--CH.sub.2 --CH.sub.2 ].sub.n --R'--Y'--S-.beta..alpha.wherein n is an integer from about 5 to about 200; R and R' are carbamate, urea, or amide; and Y and Y' are 4-phenylsuccinimido or 3-phenylsuccinimido; said process comprising intramolecularly crosslinking Hb with a compound having the formula:Y--R--CH.sub.2 --CH.sub.2 --[O--CH.sub.2 --CH.sub.2 ].sub.n --R'--Y'wherein n is an integer from about 5 to about 200; R and R' are carbamate, urea, or amide; and Y and Y' are 4-phenylmalemimido or 3-phenylmaleimido.