Patent ID: 6121020
Filing Date: 2000-09-19
Classification: A61K,C07K,C12N

Abstract:
A secreted E1/secreted E2 complex comprising:(a) a hepatitis C virus (HCV) E1 polypeptide, lacking all or a portion of its membrane spanning domain such that said E1 polypeptide is capable of secretion into growth medium when expressed recombinantly in a mammalian or yeast host cell, wherein said E1 polypeptide lacks at least a portion of its C-terminus beginning at amino acid 369 or lower, numbered with reference to the HCV1 E1 amino acid sequence (SEQ ID NO:1); and(b) a hepatitis C virus (HCV) E2 polypeptide, lacking all or a portion of its membrane spanning domain such that said E2 polypeptide is capable of secretion into growth medium when expressed recombinantly in a mammalian or yeast host cell, wherein said E2 polypeptide lacks at least a portion of its C-terminus beginning at amino acid 730 or lower, numbered with reference to the HCV1 E2 amino acid sequence (SEQ ID NO:3).