Patent ID: 7122332
Filing Date: 2006-10-17
Classification: G01N

Abstract:
1. A method for screening for a bioactive agent that modulates USP-25 protein ubiquitin-specific peptidase activity, comprising: a) combining a USP-25 protein, a USP-25 target protein which is conjugated to ubiquitin, and a candidate bioactive agent; and b) determining the level of ubiquitin-conjugated target protein in the presence and absence of said candidate bioactive agent; wherein said USP-25 protein comprises an amino acid sequence having at least about 95% identity to SEQ ID NO:2, wherein said USP-25 protein will bind to said USP-25 target protein, and wherein a difference in the level of ubiquitin-conjugated or ubiquitin-like protein-conjugated target protein in the presence and absence of said candidate bioactive agent indicates that said candidate bioactive agent modulates USP-25 protein ubiquitin-specific peptidase activity.