Patent ID: 7611866
Filing Date: 2009-11-03
Classification: C07K,C12N,C40B

Abstract:
1. A method of obtaining a bacterium comprising a nucleic acid sequence encoding an inner membrane anchor polypeptide that anchors a heterologous polypeptide to the outer side of the inner membrane of a Gram negative bacterium comprising the steps of: (a) providing a Gram negative bacterium comprising an inner membrane, an outer membrane and a periplasm; said bacterium comprising a nucleic acid sequence encoding a fusion between a heterologous polypeptide and a candidate inner membrane anchor sequence; (b) removing the outer membrane; and (c) selecting the bacterium based on the presence of the heterologous polypeptide anchored to the outer side of the inner membrane to identify an inner membrane anchor polypeptide that anchors a heterologous polypeptide to the outer side of the inner membrane of said bacterium.