Patent ID: 7667032
Filing Date: 2010-02-23
Classification: B01J,B82Y,C40B

Abstract:
1. A method of manufacturing a microarray, the method comprising: providing a substrate having a surface that is immobilized with a functional group protected with an acid-labile protecting group and capable of coupling with an oligomer probe; providing a photoacid generator onto the substrate; disposing on the substrate an imprint template comprising a convex region and a plurality of concave regions surrounding the convex region so that the convex region contacts with or is adjacent to an upper surface of the substrate to define a plurality of reaction zones by the upper surface of the substrate and the convex region and the concave regions of the imprint template, and wherein a height of each of the plurality of reaction zones is in a range from about 500 to about 4,000 nanometers (nm); exposing one or more of the reaction zones to light so that an acid is generated by the photoacid generator in the one or more exposed reaction zones and a functional group in the one or more exposed reaction zones is deprotected by the acid; and providing an oligomer probe onto the substrate so that the oligomer probe couples with the deprotected functional group.