Patent ID: 7449288
Filing Date: 2008-11-11
Classification: C07K,G01N

Abstract:
1. A method of detecting whether a target animal is Bovine Viral Diarrhea Virus positive or negative comprising: a) suspending a tissue sample comprising skin from said target animal in a diluent under conditions effective to solubilize any gp48 Bovine Viral Diarrhea Virus protein or protein fragment thereof without lysing cells; b) providing an assay system which comprises: c) analyzing said diluent in which the tissue sample comprising skin is suspended with said assay system to generate a change in signal if gp48 Bovine Viral Diarrhea Virus antigen which retains its antigenic and epitopic specificity is present in the sample compared to when no such antigen is present; and d) comparing the signal generated to one or more reference levels to indicate whether the target animal is Bovine Viral Diarrhea Virus positive or negative.