Patent ID: 6423488
Filing Date: 2002-07-23
Classification: C12N,C12Q

Abstract:
A method for the isolation of nucleic acid from nucleated red blood cells, comprising the steps of:a) obtaining a biological sample containing said nucleated red blood cells; b) adding the biological sample to a first lysis buffer to lyse plasma membranes, wherein the first lysis buffer is confined in a container that binds a precipitated nucleic acid; c) centrifuging the container to yield a supernatant and a pellet; d) removing the supernatant from the pellet in the container; e) adding a second lysis buffer to the pellet in the container, after which the pellet is incubated in the second lysis buffer for two hours to release a nucleic acid; f) precipitating the nucleic acid in the container with a nucleic acid precipitating solution; g) washing the nucleic acid in the container; h) drying the nucleic acid in the container; and i) dissolving the nucleic acid in the container in a solvent.