Patent ID: 8383358
Filing Date: 2013-02-26
Classification: A61K,A61P,G01N

Abstract:
1. A method of treating a melanoma in an animal with a chemotherapeutic regimen comprising paclitaxel, wherein said method comprises: (a) applying a first anti-SPARC antibody to a histologic section of the melanoma, wherein the first anti-SPARC antibody preferentially immunostains SPARC in tumor cells; (b) applying a second anti-SPARC antibody to the histologic section of (a) or a second histologic section of the melanoma, wherein the second anti-SPARC antibody preferentially immunostains SPARC in fibroblasts; (c) administering the chemotherapeutic regimen if the first anti-SPARC antibody and the second anti-SPARC antibody both show 3+ immunostaining of melanoma cells in the histologic section or sections; and wherein the first anti-SPARC antibody recognizes a SPARC epitope recognized by monoclonal antibody MAB941, and wherein the second antibody recognizes an immunodominant SPARC epitope recognized by polyclonal antibody AF941.