Patent ID: 8551694
Filing Date: 2013-10-08
Classification: C07K

Abstract:
1. A method for testing a compound for its ability to modulate calcium homeostasis comprising: (a) providing a host cell expressing a recombinant construct which comprises an isolated nucleic acid sequence encoding a ryanodine receptor having an amino acid sequence identity of at least 80% when compared to SEQ ID NO: 4, operably linked to at least one regulatory sequence; (b) maintaining the host cell under conditions that are suitable for expression of the recombinant construct wherein expression of the recombinant construct results in altered calcium homeostasis; (c) treating the host cell of step (a) with the compound to be tested; and (d) determining whether treatment with the compound results in a change in intracellular calcium homeostasis of the host cell; wherein such a change is an indication that the compound is able to modulate calcium homeostasis.