Patent ID: 6194149
Filing Date: 2001-02-27
Classification: C12Q

Abstract:
A method, comprising:a) providing:i) target nucleic acid comprising first and second non-contiguous single-stranded regions separated by an intervening region comprising a double-stranded region;ii) a bridging oligonucleotide capable of binding to said first and second non-contiguous single-stranded regions;iii) a second oligonucleotide capable of binding to a portion of said first non-contiguous single-stranded region; andiii) a cleavage means;b) mixing said target nucleic acid, said bridging oligonucleotide, said second oligonucleotide, and said cleavage means under conditions such that a cleavage structure is formed and either said second oligonucleotide or said bridging oligonucleotide is cleaved.