Patent ID: 8318450
Filing Date: 2012-11-27
Classification: C07D,C07F,C12Q,G01N

Abstract:
1. A method for determining the presence or absence of β-lactamase enzyme in a sample, the method comprising: a) contacting the sample with a β-lactamase substrate to form a contacted sample; b) incubating the contacted sample for a sufficient amount of time for a β-lactamase enzyme in the contacted sample to cleave the β-lactamase substrate to form an incubated sample; c) illuminating the incubated sample with an appropriate wavelength to obtain an illuminated sample ; and d) observing the illuminated sample whereby the presence or absence of β-lactamase enzyme in the illuminated sample is determined wherein the β-lactamase substrate has a structure of one of the following formulae: where R A is S, SO, or SO X is O; L—Y is a thioether linked moiety formed by reaction with a quencher moiety having a structure of formula: R R R R R R R R wherein W is a dye moiety; s is an integer from 0 to 5; W′ is (═O); W″ is OR 10 , NHR 11 , or halogen; wherein R R R