Patent ID: 9132146
Filing Date: 2015-09-15
Classification: A61K,A61P,C12N

Abstract:
1. An autologous cell vaccine comprising: a bishRNAfurin/GMCSF expression vector plasmid, wherein the vector plasmid comprises a first nucleic acid insert operably linked to a promoter, wherein the first insert encodes a Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) cDNA; a second nucleic acid insert operably linked to the promoter, wherein the second insert encodes one or more short hairpin RNAs (shRNA) capable of hybridizing to a region of a mRNA transcript encoding furin, thereby inhibiting furin expression via RNA interference wherein the shRNA incorporates cleavage dependent siRNA and cleavage independent miRNA motifs, wherein the shRNA comprises SEQ ID NO:2; and one or more optional vaccine adjuvants.