Patent ID: 8642256
Filing Date: 2014-02-04
Classification: C12Q,G01N

Abstract:
1. A method for detecting at least one substrate for at least one ubiquitination or ubiquitination-like enzyme, the method comprising: a) contacting i) the at least one ubiquitination or ubiquitination-like enzyme with ii) polypeptides immobilized on a support, wherein the polypeptides are associated with a first detectable moiety, and iii) at least one ubiquiton comprising a second detectable moiety, wherein the first moiety and the second moiety comprise a resonance energy transfer (RET) pair, b) incubating (a) under conditions to allow for ubiquitination or ubiquitination-like activity, c) exposing the support to at least one wavelength of light, and d) detecting at least one substrate for at least one ubiquitination or ubiquitination-like enzyme by detecting resonance energy transfer from the support.