Patent ID: 6410243
Filing Date: 2002-06-25
Classification: C12Q,G01N

Abstract:
A method of identifying a region of a genome of a living cell to which a protein of interest binds, comprising the steps of:a) crosslinking DNA binding protein in the living cell to genomic DNA of the living cell, thereby producing DNA binding protein crosslinked to genomic DNA; b) generating DNA fragments of the genomic DNA crosslinked to DNA binding protein in a), thereby producing a mixture comprising DNA fragments to which DNA binding protein is bound; c) removing a DNA fragment to which the protein of interest is bound from the mixture produced in b); d) separating the DNA fragment identified in c) from the protein of interest; e) amplifying the DNA fragment of d); f) combining the DNA fragment of e) with DNA comprising a sequence complementary to genomic DNA of the cell, under conditions in which hybridization between the DNA fragment and a region of the sequence complementary to genomic DNA occurs; and g) identifying the region of the sequence complementary to genomic DNA of f) to which the DNA fragment hybridzes, whereby the region identified in g) is the region of the genome in the cell to which the protein of interest binds.