Patent ID: 9150932
Filing Date: 2015-10-06
Classification: C12Q

Abstract:
1. A kit for assessing a food product for contamination, wherein said kit comprises: (a) a probe nucleic acid comprising an amplifying restriction endonuclease and a nucleotide sequence complementary to a sequence of a target nucleic acid present in a microorganism or virus, wherein at least a portion of said target nucleic acid is capable of hybridizing to at least a portion of said probe nucleic acid to form a double-stranded portion of nucleic acid comprising a restriction endonuclease cut site, (b) a first signal expansion nucleic acid comprising an amplifying restriction endonuclease and a double-stranded section having a restriction endonuclease cut site for said amplifying restriction endonuclease of said probe nucleic acid, (c) a second signal expansion nucleic acid comprising an amplifying restriction endonuclease and a double-stranded section having a restriction endonuclease cut site for said amplifying restriction endonuclease of said first signal expansion nucleic acid, and (d) a reporter nucleic acid comprising a double-stranded portion of nucleic acid comprising a restriction endonuclease cut site of said amplifying restriction endonuclease of said probe nucleic acid, said first signal expansion nucleic acid, or said second signal expansion nucleic acid.