Patent ID: 8330959
Filing Date: 2012-12-11
Classification: G01N

Abstract:
1. A surface plasmon resonance instrument comprising: A) a planar light guide defining a first side and a second side and having a thin metal film deposited on said first side, B) a flow cell adapted to flow bio-molecular fluid samples across and in contact with said thin metal film on said first side of said planar light guide, C) a first light coupler and a second light coupler adapted to couple light into and out of said planar light guide so as to produce multiple surface plasmon resonance reflections between (i) the thin metal film in contact with the bio-molecular fluid samples on the first side and (ii) the second side of said planar light guide, D) a light source for illuminating said planar light guide, E) an optical detector adapted to monitor light exiting said planar light guide, F) a computer processor programmed to analyze information provided by the optical detector to determine bio-molecular interactions without requiring flourophore labeling, wherein light intensity changes resulting from said multiple reflections are increased as compared to a single reflection.