Patent ID: 7364870
Filing Date: 2008-04-29
Classification: A61P,C07K,C12Q,G01N

Abstract:
1. A screening assay to identify compounds that inhibit or promote formation of a protein complex, comprising (i) providing a two-hybrid assay system comprising: a) a first fusion protein comprising an MK2 polypeptide, wherein said MK2 polypeptide comprises a proline-rich region, a kinase catalytic domain, a threonine residue that can be phosphorylated by MAP kinase, and a nuclear localization signal; and b) a second fusion protein comprising a Shc polypeptide set forth as SEQ ID NO: 6, under conditions wherein the two proteins interact in the two hybrid assay system; (ii) measuring a level of interaction between the fusion proteins in the presence and in the absence of a test compound; and (iii) comparing the level of interaction of the fusion proteins, wherein a difference in the amount of complex in the presence of the test compound, relative to the amount of complex in the absence of the test compound indicates that the test compound inhibits or promotes complex formation.