Patent ID: 8298767
Filing Date: 2012-10-30
Classification: C12N,C12Q

Abstract:
1. A method of sample analysis, comprising: a) annealing a reflex sequence in a polynucleotide strand in a sample with a complement of the reflex sequence present in the polynucleotide strand, wherein the polynucleotide strand comprises the following in a 5′ to 3′ orientation: b) extending from the 3′ end of the complement of the reflex sequence through the first domain to produce a double stranded region comprising a complement of the first domain at the 3′ end of the extended polynucleotide strand, thereby moving the first domain into proximity with the desired site; and c) sequencing the MID and the desired site using a primer that is complementary to the primer site in the complement of the first domain.