Patent ID: 6083485
Filing Date: 2000-07-04
Classification: A61K,C09B

Abstract:
An in-vivo diagnostic near infrared radiation method comprising:(a) administering a compound of the general formula IB.sub.1 --(F--W.sub.n).sub.m (I)whereinl represents a number from 0 to 6, n represents a number from 0 to 10 and m represents a number from 1 to 100;B is a biological detecting unit having a molecular weight of up to 30,000 that bonds to specific cell populations or selectively to receptors, or accumulates in tissues or tumours, or generally stays in the blood, or is a macromolecule that bonds non-selectively;F represents a dye showing maximum absorption in the range of 650 to 1200 nm having the general formula IIb ##STR10## wherein R.sup.3 to R.sup.12 are same or different, each independently representing a hydrogen atom, a residue B or W, or an alkyl or alkenyl residue containing up to 6 carbon atoms or an aryl or aralkyl residue containing up to 9 carbon atoms, said alkyl, alkenyl, aryl or aralkyl residue optionally carrying an additional residue W or W to each pair of adjacent residues R.sup.3 to R.sup.10 are annealed, with due regard for the interspersed atoms, 5- to 6-member rings that may be saturated, unsaturated or aromatic, and that may optionally carry residue R,R is a halogen atom, a hydroxy, carboxy, acetoxy, amino, nitro, cyano or sulfonic acid group or an alkyl, alkenyl, hydroxyalkyl, carboxyalkyl, alkoxy, alkoxycarbonyl, sulfoalkyl, alkylamino, dialkylamino or halogenalkyl residue containing up to 6 carbon atoms, an aryl, alkylaryl hydroxyaryl, carboxyaryl, sulfoaryl, arylamino, diarylamino, nitroaryl or halogenaryl residue containing up to 9 carbon atoms,s and t independently represent the numbers 0 to 1 on the condition that s and t do not represent the number 1 at the same time,X and Y are same or different, each independently representing an O, S, Se or Te or a --C(CH.sub.3).sub.2 --, --CH.dbd.CH-- or --CR.sup.13 R.sup.14 -- fragment,wherein R.sup.13 and R.sup.14 independently represent a hydrogen atom, a residue B or W, or an alkyl or alkenyl residue containing up to 6 carbon atoms or an aryl or aralkyl residue containing up to 9 carbon atoms, the alkyl, alkenyl, aryl or aralkyl residue optionally having an additional residue W;W represents a hydrophilic group that improves water-solubility, with the n-octanol-water distribution coefficient of the compound according to formula I being less than or equal to 2.0 for l=0;as well as their physiologically tolerable salts;(b) irradiating the compound with light in the near infrared range; and(c) recording the radiation produced by the compound.