Patent ID: 8802387
Filing Date: 2014-08-12
Classification: C07D,C12Q,G01N

Abstract:
1. A method of detecting beta-lactamase activity in a sample, the method comprising: (a) contacting the sample with a nanoparticulate tag, wherein the nanoparticulate tag (b) contacting the sample with a compound selected from one of general formulas (I)-(III) and (VII)-(IX): wherein R wherein X is one of O, S, Se and NH, and R R R R wherein in formulas (IV) and (VI), R and wherein the released cleavage moiety is of the formula Z-A-Z; X in formulas (III) and (IX) is one of O, S, Se and NH; E is one of S, SO, SO Z is S or Se; and A in formulas (I)-(III) and G in formulas (VII)-(IX) are each a bridge independently selected from an aliphatic, alicyclic, aromatic, arylaliphatic, and arylalicyclic radical with a main chain of 1-50 carbon atoms and 0-50 heteroatoms, wherein in the linking moiet Z-G-N the bond between Z and the bridge G and the bond between the bridge G and N are single bonds; (c) allowing beta-lactamase activity in the sample to cleave a beta-lactam moiety from the compound selected from one of general formulas (I)-(III) and (VII)-(IX) that contacted the sample in step (b), thereby releasing a cleavage moiety, the cleavage moiety being one of the formulas Z-A-Z, Z-A-Z—R (d) determining the presence of beta-lactamase activity based on the presence of the cleavage moiety immobilized onto the surface of the nanoparticulate tag.