Patent ID: 6479237
Filing Date: 2002-11-12
Classification: C07K,C12N,C12Q

Abstract:
A method for detecting an interaction or an absence of interaction between a first protein and at least two other proteins, said interaction being (i) formation of a protein complex including said first protein and said at least two other proteins, or (ii) formation of a protein complex including said at least two other proteins, said formation being catalyzed by said first protein, or (iii) inhibition of formation of a protein complex among said at least two other proteins wherein the inhibition of protein complex formation is mediated by said first protein; said method comprising:(a) transforming host cells with: i) a DNA sequence encoding said first protein, wherein transcription of said DNA sequence is controlled by a conditional promoter, and ii) DNA sequences encoding said at least two other proteins, wherein the genome of said host cells contains at least one reporter gene that is transcribed in the presence of a protein complex comprising the at least two other proteins, (b) comparing effects of repression of said conditional promoter with effects of activation of said conditional promoter on transcription of said at least one reporter gene, and (c) correlating the results of step (b) with detecting an interaction or the absence of interaction between said first protein and said at least two other proteins.