Patent ID: 7811821
Filing Date: 2010-10-12
Classification: A61P,C12N

Abstract:
1. A method of culturing human embryonic stem cells into immature dendritic cells, the method comprising the steps of (a) co-culturing human embryonic stem cells with stromal cells that do not express macrophage colony-stimulating factor, wherein the stem cells are induced to differentiate into multipotent lympho-hematopoietic progenitor cells and wherein the culture is free of exogenously added cytokines; (b) culturing the progenitor cells with granulocyte/macrophage colony stimulating factor (GM-CSF) under non-adherent conditions to cause the expansion of myeloid precursors cells, wherein at least 90% of the cells obtained are CD45 (c) further differentiating the myeloid precursors in the presence of GM-CSF and IL-4 to form cells that have the phenotype of immature dendritic cells; and (d) recovering cells which have the phenotype of immature dendritic cells, wherein the immature dendritic cells are of myeloid lineage.