Patent ID: 7332296
Filing Date: 2008-02-19
Classification: G01N,Y10T

Abstract:
1. A method of quantifying the enzymatic activity of at least one enzyme selected from the group consisting of renin, ACE1 and ACE2 with a known substrate, the method comprising: a) obtaining a biological sample comprising said at least one enzyme; b) adding at least one known substrate of said at least one enzyme to the biological sample and incubating to generate enzymatic activity products in a mixture; c) loading the mixture onto a protein chip to produce a loaded chip; d) washing the loaded chip to remove any unbound proteins and contaminants; e) reading the loaded chip using SELDI-TOF mass spectrometry; f) generating spectral data using commercially available software compatible for this purpose; g) analyzing the spectral data to identify the enzymatic activity products; and h) subjecting the spectral data to an algorithm that permits quantification of the enzymatic activity products.