Patent ID: 9024006
Filing Date: 2015-05-05
Classification: C12Q

Abstract:
1. A reaction mixture comprising: (a) amplification reagents comprising a thermostable polymerase, nucleotides, a first primer and a second primer, wherein the first primer and the second primer amplify a target genomic locus from a nucleic acid sample comprising human genomic DNA and said first primer comprises: (b) flap assay reagents comprising a flap endonuclease, a FRET cassette and a flap oligonucleotide that comprises a nucleotide that base pairs with said point mutation; (c) said nucleic acid sample, wherein said nucleic acid sample comprises both wild type copies of said target genomic locus and mutant copies of said target genomic locus and wherein the mutant copies of said target genomic locus have said point mutation of step (a)(ii); wherein the reaction mixture does not comprise an invasive oligonucleotide distinct from said first primer and wherein said reaction mixture is characterized in that it can amplify and detect the presence of said mutant copies of said target genomic locus in said sample.