Patent ID: 6235488
Filing Date: 2001-05-22
Classification: G01N,Y10S

Abstract:
A method for rendering a piezoelectric crystal substrate surface containing a layer of silicon dioxide having hydroxyl groups thereon specific for the binding of a selected substance, said method comprising the steps of:attaching to the surface of said substrate a layer of coupling agent which binds the layer to said substrate and which possesses binding sites for binding a ligand-binding substance thereto wherein said coupling agent is a silyl compound of the formula:R.sub.n SiX.sub.(4-n)wherein R is a nonhydrolyzable organic radical containing a primary alcohol group, X is a hydrolyzable moiety selected from the group consisting of chloro, amino, alkoxy, and acyloxy, and n is an integer from 1 to 3;attaching a single layer of ligand-binding substance to said layer of coupling agent, said layer of ligand-binding substance having reciprocal binding sites available for binding said ligand-binding substance to said coupling agent as well as binding sites available for binding said ligand-binding substance to a ligand-bearing substance; andattaching a single layer of ligand-bearing substance to said ligand-binding layer, said ligand-bearing substance having reciprocal binding sites available for binding said ligand-bearing substance to said ligand-binding substance as well as binding sites for binding of the selected substance.