Patent ID: 9127300
Filing Date: 2015-09-08
Classification: C12M,C12Q

Abstract:
1. A method for detecting the presence or absence of an aerobic or a facultatively anaerobic microorganism in a sample, comprising: providing a sample and a culture device comprising a base member, a cover layer, and a hydrogel comprising a plurality of abiogenic gas bubbles disposed there between; inoculating the growth area of the device with the sample at a first point in time; incubating the device for a period of time; illuminating the growth area with a light source; observing an abiogenic gas bubble in the hydrogel in the growth area at a second point in time; observing the growth area with regard to the size or absence of the abiogenic gas bubble at a third point in time, wherein the third point in time occurs after the second point in time; comparing observations of the growth area at two points in time; and detecting the presence or absence of an aerobic or a facultatively anaerobic microorganism in the growth area at a third point in time; wherein detecting the presence or absence of an aerobic or a facultatively anaerobic microorganism comprises observing an indication of growth; wherein observing an indication of growth comprises detecting a diminution or absence of the abiogenic gas bubble in the hydrogel at the third point in time; wherein detecting the diminution or absence of the abiogenic gas bubble in the hydrogel at the third point in time indicates the presence of an aerobic or a facultatively anaerobic microorganism in the sample.