Patent ID: 9023603
Filing Date: 2015-05-05
Classification: A61K,C07K,C12N

Abstract:
1. A method for producing influenza virus particles for preparation of a vaccine, comprising: (a) introducing expression vectors into cultured cells in the absence of helper virus, wherein said expression vectors comprise influenza genomic vRNA segments or antigenomic cRNAs of said genomic vRNA segments, sufficient to produce in said cells the genomic vRNA segments or antigenomic cRNAs of said genomic vRNA segments; (b) introducing into said cultured cells protein expression vectors encoding a nucleoprotein and RNA-dependent RNA polymerase subunits, wherein ribonucleoprotein complexes (RNPs) containing the genomic vRNA segments of said influenza viruses are formed; and (c) recovering said influenza virus particles.