Patent ID: 7820394
Filing Date: 2010-10-26
Classification: G01N

Abstract:
1. A method for detecting and quantifying the presence of a target analyte of interest by a high gain redox chemical amplification reaction having an amplification factor of 10 7 to 10 9 , said method comprising the steps of: selecting a biomarker that comprises a metal catalyst or a metal catalyst precursor and a moiety that is selectively reactive with the target analyte of interest; reacting said target analyte of interest with said reactive moiety of said biomarker to selectively form a first reaction product comprising said metal catalyst or a metal catalyst precursor contained in a reaction mixture; separating the reaction mixture into two or more fractions; and detecting and quantifying the target analyte of interest subjecting said first reaction product present in at least one of said fractions to a high gain redox chemical amplification formulation comprising a leuco dye and a reducing agent to produce a second reaction product in the form of a dye, said dye being formed in a reaction catalyzed by said first reaction product.