Patent ID: 6093570
Filing Date: 2000-07-25
Classification: C07K,C12N

Abstract:
A method for producing substantially helper-virus free stocks of recombinant adeno-associated virus comprising:(a) cotransfecting cells permissive for adenovirus-associated virus replication with:i) a recombinant adeno-associated virus vector which is capable of being packaged into infectious AAV virions,ii) a helper AAV vector which provides the AAV-viral functions essential for the replication and packaging of said recombinant adeno-associated virus vector into infectious AAV virions, andiii) a helper virus vector which provides the helper-viral functions essential for a productive adeno-associated virus infection but which cannot itself be packaged into infectious helper virus virions,thereby producing infective virions of recombinant AAV in vivo without a concomitant infection by helper virus; and,(b) collecting virions produced without conducting any process to inactivate helper virus.