Patent ID: 6180395
Filing Date: 2001-01-30
Classification: B01L,C12Q,G01N,Y10S

Abstract:
A unit dose reagent chamber for use in a test apparatus for the detection of adenosine triphosphate (ATP) or alkaline phosphatase (AP) in a test sample, and wherein a moveable probe is employed to obtain a test sample and to release reagents from the reagent chamber to a test unit, which unit dose chamber comprises:a) a cylinder having a one open end and an other opposite open end;b) a probe-puncturable membrane seal over the one end and the other end of the cylinder to form a sealed compartment; andc) a reagent composition for use in the detection of the test sample and sealed within the sealed compartment, which composition consists essentially of and is selected from the group consisting of:i) a detergent-containing buffered solution to release adenosine triphosphate (ATP) or alkaline phosphatase (AP) from the test sample into the solution for testing;ii) a reaction stopping solution having a pH of 8 to 11; andiii) a luciferin-luciferase or phosphatase substrate reagent tablet.