Patent ID: 6413774
Filing Date: 2002-07-02
Classification: C07K,C12N,C12Q,C12Y,C40B,H01L

Abstract:
A method of producing a recombinant nucleic acid with a desired property from at least two nucleic acids, the method comprising:(a) providing single-stranded segments of the at least two nucleic acids; (b) hybridizing the single-stranded segments to produce partially or fully annealed nucleic acid strands and elongating the partially or fully annealed nucleic acid strands to produce recombinant nucleic acids; (c) denaturing the recombinant nucleic acids to form recombinant single-stranded nucleic acids; (d) hybridizing the single-stranded recombinant nucleic acids of step (c) to produce additional partially or fully annealed nucleic acid strands; (e) elongating the partially or fully-annealed nucleic acid strands of step (d) to produce transferrable recombinant nucleic acids; (f) transferring the transferrable recombinant nucleic acids into one or more cells; and, (g) recombining the transferrable recombinant nucleic acids in the one or more cells to produce additional recombinant nucleic acids.