Patent ID: 6703215
Filing Date: 2004-03-09
Classification: A61K,A61P

Abstract:
A method for screening an inhibitor of an inositol polyphosphate 5-phosphatase of SEQ ID No. 1, said method comprising:(a) incubating a mixture of inositol polyphosphate substrate and a sample comprising said 5-phosphatase, in the presence and absence of a test compound; (b) determining the amount of a 5-phosphatase hydrolysis product formed in the presence and absence of said test compound; and (c) comparing the amount of said 5-phosphatase hydrolysis product in the presence of said test compound to the amount of said 5-phosphatase hydrolysis product in the absence of said test compound, wherein a decrease in the amount of a product of 5-phosphatase activity in the presence of the test compound is indicative of said test compound being an inhibitor of said 5-phosphatase.