Patent ID: 6331402
Filing Date: 2001-12-18
Classification: G01N

Abstract:
A method for the detection of an analyte in a biological sample suspected of containing said analyte and an interfering substance, said method comprising the steps of:a. forming a test mixture comprisingi) said sample,ii) a binding partner specific for said analyte, andiii) an interference reducing substance in a concentration of at least about 10 .mu.g/ml of the test mixture, said substance comprising a peptide sequence derived from framework region 1 or 3 of the variable domain of an antibody selected from the group consisting of said binding partner and antibodies used for immunological therapy or scintigraphy,wherein said interference reducing substance is not mouse serum and wherein said test mixture allows for binding of said analyte to said binding partner, andb. detecting said analyte present in said sample by measuring the binding between said analyte and said binding partner.