Patent ID: 7867711
Filing Date: 2011-01-11
Classification: C12Q

Abstract:
1. A probe-immobilized carrier, for specifically detecting a gene of infectious disease pathogenic bacterium, Propionibacterium acnes , in a sample potentially containing a gene of Propionibacterium avidum , the probe-immobilized carrier having a region where a probe set comprising three isolated and purified probes consisting of the following probes (A) to (C) respectively is immobilized on a solid-phase carrier as arranged at intervals so that the gene of Propionibacterium acnes capable of reacting with all of the three probes can be detected as precisely distinguished from the gene of Propionibacterium avidum when the sample is brought into contact with the probe-immobilized carrier under stringent conditions: (A) a probe consisting of GGGCTAATACCGGATAGGAGCTCCTG (SEQ ID NO. 87) or the complementary sequence thereof; (B) a probe consisting of AAGCGTGAGTGACGGTAATGGGTAAA (SEQ ID NO. 88) or the complementary sequence thereof; and (C) a probe consisting of ATCGCGTCGGAAGTGTAATCTTGGG (SEQ ID NO. 89) or the complementary sequence thereof.