Patent ID: 7504216
Filing Date: 2009-03-17
Classification: C12N

Abstract:
1. A method for determining whether a test DNA molecule interacts with a test polypeptide, the method comprising (a) providing a first population of prokaryotic host cells, wherein at least one of the host cells comprises; (b) maintaining the first population of host cells under selective conditions that allow cell growth as a result of the expression of the selectable reporter gene; (c) isolating the reporter vectors from cells of the first population of host cells that grow under the selective conditions; (d) introducing the isolated reporter vectors into a second population of host cells that lack the chimeric gene; (e) maintaining the second population of host cells under counterselective conditions that inhibit cell growth as a result of the expression of the counterselectable reporter gene; and (f) measuring growth of the second population of host cells, wherein an ability to survive under counterselective conditions indicates that the test DNA molecule has interacted with the test polypeptide.