Patent ID: 8815503
Filing Date: 2014-08-26
Classification: A61K,A61P,C12N

Abstract:
1. A method for preparing an antisense RNA oligonucleotide having gene silencing activity and high IL-12 inducing activity, comprising the steps of: (a) identifying all potential antisense sequences between 18 and 50 nucleotides in length, for a target mRNA; (b) identifying antisense sequences that have gene silencing activity; (c) identifying antisense RNA sequences from those among identified in step (b) which have an IL-12 score of at least 8.4064×n+66.958, wherein the IL-12 score is assigned according to the method comprising the steps of: and wherein n is the length of the sequence and n is between 18 and 50; (d) decreasing the IL-12 score threshold by 1 if no antisense sequence is identified in step (c), until at least one antisense sequence is identified; (e) preparing the antisense RNA identified in step (c) or (d); and (f) optionally testing the gene silencing and/or the IL-12 inducing activity of the antisense prepared in (e).