Patent ID: 8609339
Filing Date: 2013-12-17
Classification: C12Q

Abstract:
1. A method for extraction of solid-phase immobilized nucleic acids from an emulsion, comprising the steps of: a) breaking an emulsion comprising a plurality of aqueous droplets in a continuous phase of an oil by application of an organic solvent to produce a combined aqueous-oil mixture, wherein the organic solvent disrupts the aqueous droplets releasing a plurality of nucleic acids each immobilized on a bead substrate into the broken emulsion mixture; b) introducing an inorganic salt to the broken emulsion mixture causing a phase separation of the mixture into a first phase comprising an aqueous solution and the bead substrate immobilized nucleic acids and a second phase comprising the solvent and the oil; c) extracting the first phase from the second phase; and d) collecting the bead substrate immobilized nucleic acids from the first phase.