Patent ID: 7885767
Filing Date: 2011-02-08
Classification: C12Q

Abstract:
1. A method to quantify an initial population of nucleic acids in a sample of interest subjected to a population amplification reaction comprising a succession of repeated amplification cycles, the method comprising performing by means of an apparatus experimental measurements representative of a current size of the population of the sample of interest during the repeated amplification cycles and further comprising the following steps: a) providing a model of the yield of the amplification reaction, this model corresponding to the variation of the yield in function of the number of amplification cycles and being represented by a curve having: b) determining a relationship between the changeover index C where N c) determining an experimental variation of the yield and a changeover index value from the experimental measurements performed by said apparatus during the repeated amplification cycles, and d) determining the initial population size in the sample of interest from the changeover index value by means of the relationship between the changeover index and the said parameter representative of the initial population size.