Patent ID: 9222082
Filing Date: 2015-12-29
Classification: C07K,C12N

Abstract:
1. A method of covalently coupling two or more proteins, the method comprising: a) providing a first protein having covalently attached thereto (i) at least one first linker comprising a first hybridizable region and (ii) at least one first group capable of forming a covalent bond wherein the first group is covalently attached to the first protein via the first linker; b) providing a second protein having covalently attached thereto (i) at least one second linker comprising a second hybridizable region capable of hybridizing to the first hybridizable region and (ii) at least one second group capable of forming a covalent bond with the first group wherein the second group is covalently attached to the second protein via the second linker; c) contacting the first and second proteins under conditions that allow the first and second hybridizable regions to hybridize and link the proteins; and d) exposing the linked proteins to conditions that allow the formation of a covalent bond between the first and second groups.