Patent ID: 7683035
Filing Date: 2010-03-23
Classification: C12N,Y10T

Abstract:
1. A method of stabilizing unpurified nucleic acids in a biological sample from degradation comprising: contacting a biological sample containing unpurified nucleic acid with at least one cationic compound of formula (I) wherein (A) is an anion, and said anion is a conjugated base of a strong or weak inorganic or organic acid, or combinations thereof, and wherein the cationic compound (I) and anion (A), together, have a neutral charge, and wherein X is nitrogen (N), k is the integer 1, 2, 3, or 4, B represents a substituted or non-substituted aliphatic alkanediyl bridge, wherein the substituted aliphatic alkanediyl bridge is substituted on one or more carbon atoms, wherein one or more non-adjacent carbon atoms is replaced by oxygen, and wherein the substituted aliphatic alkanediyl bridge is the structure wherein n and m independently represent the integer 0, 1, 2, 3, 4, 5, or 6, with n+m>0; R methyl, ethyl, propyl, isopropyl, butyl, isobutyl, n-pentyl, isopentyl, and hexyl R and a structure wherein n, m are independent of each other, and n represents the integer 2, 3 or 4, m represents the integer 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, or 18, and Z represents —O—, —CO—, —OCO—, —CO—N—, or —N—CO—; wherein the unpurified nucleic acid in the biological sample forms a complex with the cationic compound (1) and is stabilized from degradation within said biological sample; optionally concentrating the complexes of nucleic acid and cationic compound; storing the complex of nucleic acid and cationic compound until further tests are conducted or a further purification is performed; and optionally, isolating the stabilized nucleic acid from the biological sample.