Patent ID: 6197495
Filing Date: 2001-03-06
Classification: C07K,C12N

Abstract:
A method of identifying an inhibitor compound capable of binding to, and inhibiting the enzymatic activity of a Staphylococcus glycyl tRNA synthetase, said method comprising:introducing into a suitable computer program information defining an active site conformation of a Staphylococcus glycyl tRNA synthetase molecule comprising a conformation defined by the coordinates of FIG. 1, wherein said program displays the three-dimensional structure thereof;creating a three dimensional structure of a test compound in said computer program;displaying and superimposing the model of said test compound on the model of said active site;assessing whether said test compound model fits spatially into the active site;incorporating said test compound in a biological synthetase activity assay for a synthetase characterized by said active site; anddetermining whether said test compound inhibits enzymatic activity in said assay.