Patent ID: 7625873
Filing Date: 2009-12-01
Classification: A61P,C07F,C12N

Abstract:
1. A method of inhibiting the growth of Escherichia coli, Salmonella typhimurium , or Yersinia pestis pathogenic bacterial cells, comprising exposing the bacterial cells to a growth-inhibiting amount of an antisense oligonucleotide compound, composed of morpholino subunits and phosphorus-containing intersubunit linkages joining a morpholino nitrogen of one subunit to a 5′ exocyclic carbon of an adjacent subunit, and having: (i) no more than 12 nucleotide bases, (ii) a targeting nucleic acid sequence of no fewer than 10 bases in length that is complementary to a target sequence containing or within 10 bases, in a downstream direction, of the translational start codon in SEQ ID NO: 2; and (iii) a T wherein said morpholino subunits in the oligonucleotide compound are joined by a combination of (a) uncharged phosphorodiamidate linkages, in accordance with structure I,  where Y (b) cationic phosphorodiamidate linkages in accordance with structure I, where Y and wherein said cationic linkages make up no more than about half the total number of linkages.