Patent ID: 6281408
Filing Date: 2001-08-28
Classification: A01K

Abstract:
A method of producing a compound chimeric mouse, comprising:(a) obtaining mouse embryonic stem (ES) cells whose genomes' comprise a construct comprising a nucleic acid sequence encoding a marker gene and a gene of interest each of which is operatively linked to a promoter;(b) selecting said ES cells of (a) that functionally express said marker gene;(c) cloning said ES cells of (b) to obtain individual ES cell clones;(d) analyzing the site of integration of each of said individual ES cell clones of (c);(e) selecting at least two of said individual ES cell clones of (d) with different sites of integration;(f) mixing said individual ES cell clones selected in (e) to form a mixed pool of ES cell clones with different integration sites;(g) co-culturing said mixed pool of ES cell clones with a mouse morula having a zona pellucida removed such that compound chimeric mouse embryos are formed;(h) transferring said compound chimeric mouse embryos to a pseudopregnant female; and(i) obtaining a compound chimeric mouse comprising cells that express said gene of interest to detectable levels.