Patent ID: 8980284
Filing Date: 2015-03-17
Classification: A61K,A61P,C07K,C12N,G01N,Y02A

Abstract:
1. An isolated Clostridium botulinum neurotoxin propeptide comprising: a light chain region; a heavy chain region, wherein the light and heavy chain regions are linked by a disulfide bond; an intermediate region connecting the light and heavy chain regions and comprising a highly specific protease cleavage site, wherein said highly specific protease cleavage site has three or more specific adjacent amino acid residues that are recognized by the highly specific protease in order to enable cleavage; and a cargo attachment peptide sequence to enable site-specific attachment of cargo, wherein the cargo attachment peptide sequence is positioned upstream of the light chain region and is separated from the N-terminus of the light chain region by an amino acid spacer sequence.