Patent ID: 7067715
Filing Date: 2006-06-27
Classification: A01K

Abstract:
1. A method of producing a transgenic mouse comprising: introducing a vector into a mouse embryo or a mouse ES cell and transferring said ES cell into a blastocyst; transferring said embryo or said blastocyst comprising said ES cell into a pseudopregnant female mouse; allowing said embryo or blastocyst to develop into an offspring; selecting an offspring that expresses agouti cDNA and has a coat color phenotype; wherein said vector comprises: a first transgene expression cassette comprising mouse agouti cDNA operably linked to a human keratinocyte specific K14 promoter, a second transgene expression cassette comprising RNA polymerase II large subunit promoter, and a chicken beta-globin HS4 insulator; wherein said insulator and said first transgene expression cassette are located at the 5′ or 3′ end of said second transgene expression cassette; the number of copies of said chicken beta-globin HS4 insulator is 1–6; and said insulator is in the same or opposite orientation relative to said first and second transgene expression cassettes in said vector.