Patent ID: 9206417
Filing Date: 2015-12-08
Classification: C12N,C12Q

Abstract:
1. A method for identifying a genomic region of interest from a single source in a sample comprising DNA from two different sources, comprising the steps of: providing a sample comprising DNA from two different sources; introducing to the sample a first set of oligonucleotide probes comprising a first fixed sequence oligonucleotide complementary to a 3′ region in a genomic region of interest, a second fixed sequence oligonucleotide complementary to a 5′ region in the genomic region of interest, and one or more bridging oligonucleotides that hybridize to the genomic region of interest between and adjacent to the first and second fixed sequence oligonucleotides of the first set of oligonucleotide probes; hybridizing the first set of oligonucleotide probes to the genomic region of interest in the sample; ligating the hybridized oligonucleotides of the first set of oligonucleotide probes to create first ligation products complementary to the genomic region of interest; introducing to the first ligation products a second set of oligonucleotide probes comprising a first fixed sequence oligonucleotide complementary to a 3′ region in the first ligation product, a second fixed sequence oligonucleotide complementary to a 5′ region in the first ligation product, and one or more bridging oligonucleotides that hybridize to the first ligation product between and adjacent to the first and second fixed sequence oligonucleotides of the second set of oligonucleotide probes; hybridizing the second set of oligonucleotide probes to the first ligation products; ligating the hybridized oligonucleotides of the second set to create second ligation products complementary to the first ligation products; amplifying the second ligation products to create amplification products; and analyzing the amplification products, wherein analysis of the amplification products identifies the genomic region of interest from the single source in the sample.