Patent ID: 8753824
Filing Date: 2014-06-17
Classification: G01N

Abstract:
1. A method for detecting at least two antigens in a cell or tissue sample, said method comprising: a. obtaining a cell or tissue sample; b. processing said cell or tissue sample for antigen detection wherein said detection is on a slide; c. labeling and detecting at least one antigen in said cell or tissue sample and capturing an image of said at least one antigen, and optionally simultaneously detecting at least two labeled antigens, wherein said labeling is selected from the group consisting of immunohistochemical, immunogold, and in situ labeling; d. erasing said label from said cell or tissue sample, wherein said label comprises a chromogenic substrate and said substrate is soluble in an organic alcohol solvent; e. processing said cell or tissue sample to retrieve said at least one antigen using microwave antigen retrieval; f. reprobing said cell or tissue sample by labeling and detecting at least one different antigen in said cell or tissue sample and capturing an image of said at least one different antigen; g. optionally determining the level of at least one antigen of step c and optionally determining the level of at least one antigen of step f; h. optionally counterstaining said cell or tissue before or after detection of said antigens; and i. scanning said slide using a whole slide digital scanning microscope after said cell or tissue sample is stained or labeled, capturing a digital image of said stained or labeled cell or tissue sample, importing said digital image into a program for processing images, and optionally visualizing said image; thereby detecting at least two antigens in a cell or tissue sample.