Patent ID: 8986964
Filing Date: 2015-03-24
Classification: C12N,C12P,C12R,C12Y,Y02E

Abstract:
1. A method for production of ethanol, comprising: culturing an engineered cyanobacterium in a culture medium in the presence of light and inorganic carbon, wherein said engineered cyanobacterium comprising a recombinant pyruvate decarboxylase nucleic acid sequence encoding a pyruvate decarboxylase having EC 4.1.1.1 and a plurality of recombinant alcohol dehydrogenase nucleic acid sequences encoding an alcohol dehydrogenase having EC 1.1.1.1 or EC 1.1.1.2, wherein the expression level the alcohol dehydrogenase and the expression level of the pyruvate decarboxylase are regulated by separate promoters, wherein the expression level of alcohol dehydrogenase in the engineered cyanobacterium is greater than the expression level of alcohol dehydrogenase in a control engineered cyanobacterium comprising a recombinant alcohol dehydrogenase nucleic acid sequence and a recombinant pyruvate decarboxylase nucleic acid sequence regulated together by a single promoter, and wherein the engineered cyanobacterium produces ethanol in an amount greater than a non-engineered cyanobacterium , when cultured under identical conditions.