Patent ID: 9005886
Filing Date: 2015-04-14
Classification: A01N

Abstract:
1. A method for cryopreservation of human spermatozoa free from seminal plasma using an aseptic vitrification-devitrification process, wherein said method comprises: (a) providing spermatozoa free from seminal liquid; (b) resuspending and mixing the spermatozoa obtained in step (a) in a 1:1 volumetric ratio with a vitrification medium comprising: (c) mixing and adjusting the final concentration of spermatozoa within a range from 0.2×10 (d) placing 100 μl of the mixture of spermatozoa and vitrification medium immediately after step (c) in a 0.25 mL straw, then placing this straw inside a 0.5 mL straw having an open end; (e) immersing the 0.5 ml straw with the sperm content in horizontal position in liquid N (f) keeping the sperm sample inside the straws at a temperature lower than −75° C; (g) devitrifying the sample by introducing the straw containing the sperm sample in a devitrification medium comprising: