Patent ID: 9222936
Filing Date: 2015-12-29
Classification: C12Q,G01N

Abstract:
1. A method for suppressing cross-hybridization in assaying one or more targets of a sample comprising i) hybridizing ii) blocking the hybridized molecular probe-detectable component with a blocking component comprising iii) combining the blocked hybridized molecular probe-detectable component with one or more blocked hybridized molecular probe-detectable components similarly prepared by steps i) through ii) to yield a mixture of the blocked hybridized molecular probe-detectable components; iv) combining the mixture of the blocked hybridized molecular probe-detectable components with the sample comprising the one or more targets; v) binding the one or more targets in the sample with one or more of the binding moieties of the molecular probes comprising the mixture of the blocked hybridized molecular probe-detectable components; vi) detecting a signal generated from the one or more targets bound to the one or more of the molecular probes that comprise the mixture of the blocked hybridized molecular probe-detectable components; wherein the method further is characterized by one or more of