Patent ID: 8771933
Filing Date: 2014-07-08
Classification: C12M,C12N,C12Q,G01N

Abstract:
1. A method of sorting a fluid mixture comprising a plurality of cells, said method comprising the steps of: a) loading the fluid mixture comprising a plurality of deformable and non-deformable cells in a microfluidic cell sorter comprising: b) applying a force field diagonally with respect to the length of said trenches, whereby applying said force field allows diagonal fluid flow with respect to the shallow regions or slits for separation of said plurality of cells, wherein said deformable cells deform while crossing from one trench to another through said shallow regions or slits, and said non-deformable cells slide along said shallow regions or slits in a direction parallel to said trenches resulting in a continuous sorting of said deformable and non-deformable cells, and ensuring that said spacing is smaller than the smallest dimension of the cells; and c) collecting separated cells obtained in (b) from said sample outlet.