Patent ID: 8945895
Filing Date: 2015-02-03
Classification: A61P,C12M,C12N,C12Y

Abstract:
1. A method for purifying recombinant a disintegrin-like and metallopeptidase with thrombospondin type I motif 13 (ADAMTS13) protein from a sample comprising ADAMTS13 protein and non-ADAMTS13 impurities, the method comprising the steps of: (a) chromatographically contacting the sample with hydroxyapatite under conditions that allow said ADAMTS13 protein to appear in a flow through fraction from said hydroxyapatite by said ADAMTS13 protein not binding to said hydroxyapatite while said non-ADAMTS13 impurities are retained on said hydroxyapatite; (b) subsequently chromatographically contacting said flow through fraction with a mixed mode cation exchange/hydrophobic interaction resin that hinds said ADAMTS13 protein; and (c) eluting said ADAMTS13 protein from said mixed mode cation exchange/hydrophobic interaction resin with an elution buffer.