Patent ID: 8530201
Filing Date: 2013-09-10
Classification: C12N,C12P,C12Y,Y02E

Abstract:
1. A method of converting a biomass substrate to a fermentable sugar, the method comprising contacting the biomass substrate with a recombinant β-glucosidase polypeptide variant having beta-glucosidase activity, under conditions suitable for the production of the fermentable sugar from said biomass substrate, wherein the recombinant β-glucosidase polypeptide variant comprises: (a) an amino acid sequence that has at least 85% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 4, wherein SEQ ID NO: 4 is the amino acid sequence of wild type (b) an amino acid sequence encoded by a nucleic acid that hybridizes under highly stringent conditions with the complementary strand of a polynucleotide encoding the amino acid sequence of SEQ ID NO: 4, wherein the highly stringent conditions comprise prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/mL sheared and denatured salmon sperm DNA and 50% formamide and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C., wherein the encoded amino acid sequence has a substitution or deletion of the amino acid residue F211, wherein amino acid position is numbered with reference to the amino acid sequence of SEQ ID NO: 4, and wherein the nucleic acid encodes a polypeptide having beta-glucosidase activity.