Patent ID: 8986282
Filing Date: 2015-03-24
Classification: A61K,A61M

Abstract:
1. A method for transferring a macromolecular complex to muscle cells, said method comprising the steps of: (a) isolating a region of the subject's microvasculature; and (b) delivering a macromolecular complex to the venous side of the isolated region of the subject's microvasculature under about 50 mm Hg to about 500 mm Hg hydrostatic pressure, wherein said hydrostatic pressure transfers said macromolecular complex to the muscle cells, wherein said macromolecular complex comprises a viral vector carrying a target molecule, wherein said viral vector is a recombinant adenoviral (rAd) vector or a recombinant adeno-associated viral (rAAV) vector, wherein said macromolecular complex is delivered substantially in the absence of vascular permeability-enhancing agents.