Patent ID: 8372653
Filing Date: 2013-02-12
Classification: G01N,Y10T

Abstract:
1. A method of analyzing an analyte, comprising: covalently bonding a mass tag to an analyte to form a labeled analyte; subjecting the labeled analyte to a first step of mass spectrometry fragmentation under first conditions that do not dissociate the labeled analyte; subjecting the labeled analyte to a second step of mass spectrometry fragmentation under second conditions that differ from the first conditions, the second step of mass spectrometry fragmentation forming a mass spectrum and the second conditions comprising conditions that dissociate the labeled analyte into a charged, signature ion-carrying analyte and a separated neutral loss group; quantitating the analyte based on the mass spectrum; subjecting the charged, signature ion-carrying analyte to a third step of mass spectrometry fragmentation under third conditions that differ from the first conditions and differ from the second conditions, the third step of mass spectrometry fragmentation forming a second mass spectrum and the third conditions comprising conditions that dissociate the charged, signature ion-carrying analyte into a plurality of charged fragments; and identifying the analyte based on the second mass spectrum.