Patent ID: 6177248
Filing Date: 2001-01-23
Classification: C12Q,Y02A

Abstract:
A method for detecting a WT1 gene functional mutation in target cells comprising the steps of:detecting expression of at least one down-stream gene of WT1 in a sample of (a) target cells, and (b) reference cells having a wild-type WT1 gene, wherein the reference cells are otherwise substantially similar to the target cells, the down-stream genes are up- or down-regulated by the wild-type WT1 gene, wherein said down-stream genes comprise at least one WT1 up-regulated gene selected from the group consisting of: natural killer cells protein 4 precursor (M59807), folate binding protein (M25317), HALPHA44 gene for alpha-tubulin (X06956), 90 K product (H17969), amphiregulin (M30704), procollagen alpha 1 (T51558), beta-migrating plasminogen activator inhibitor 1 (M14083), cysteine protease inhibitor from radiated keratinocytes (X05978), brain natriuretic protein (BNP) (M31766), leukemia virus receptor 1 (GLVR1 ) (L20859), type 1 cytoskeletal 17 keratin (R71870), tubulin alpha-5 chain (H45051), purine nucleoside phosphorylase (T47964), Gem GTPase (U10550), adrenomedullin (D14874), GPI-anchored urokinase plasminogen activator surface receptor (R38636), tissue type plasminogen activator (X13097), gravin (M96322), jun-B(X51345), homeotic gene regulator (R16977), clone 9112 (X57348), interferon gamma treatment inducible mRNA (M26683), transmembrane receptor protein (Z17227) and mitochondrial phosphate carrier protein (R49231), and wherein said WT1 down-regulated gene is elongation factor 1 alpha-2 (X79490); andcomparing the expression of the down-stream genes in the target cells and the reference cells, wherein a difference in the expression between the target cells and reference cells suggests a WT1 functional mutation in the target cells.