Patent ID: 6838264
Filing Date: 2005-01-04
Classification: A61K,C07K

Abstract:
1. A method for producing an antibody to the N-terminal portion of (1-84) PTH useful in the determination of intact PTH 1-84 levels in a biological sample, the method comprising the steps: a) administering a first peptide antigen alone or a first peptide antigen coupled to a carrier protein to a host animal to induce antibody production against said first peptide antigen in said host animal, said first peptide antigen being selected from the group consisting of SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, (1-34) PTH and (1-84) PTH; b) monitoring antibody titer produced by said administration of said peptide antigen to said host animal; c) extracting antisera produced in said host animal; and d) isolating and selecting at least one antibody from said antisera extracted in step c) by affinity chromatography utilizing a second peptide antigen selected from the group consisting of SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, and SEQ ID NO. 6.