Patent ID: 6855534
Filing Date: 2005-02-15
Classification: C12N

Abstract:
1. A method for making an infectious adenovirus having enhanced efficiency which comprises contacting a cell with or introducing into a cell: (a) a first nucleic acid sequence encoding adenovirus sequences which, in the absence of intermolecular recombination, are incapable to encode an infectious, replicable or packageable adenovirus; and (b) a second nucleic acid sequence encoding adenovirus sequences which, in the absence of adenoviral replication factors provided in trans or intermolecular recombination with said first nucleic acid sequence, are incapable to encode an infectious, replicable or packageable adenovirus; provided that said first and said second nucleic acid sequences each comprise a head-to-head ITR junction and said first nucleic acid and said second nucleic acid comprise recombinase recognition sites and wherein said first and said second nucleic acids are contacted with a recombinase which recognizes said first nucleic acid and said second nucleic acid recombinase recognition sites; whereby said first and said second nucleic acids recombine to form said infectious adenovirus.