Patent ID: 7718575
Filing Date: 2010-05-18
Classification: A61P,C07K,G01N

Abstract:
1. A method of selecting at least one test molecule that binds to HLA-DP4, comprising: (i) incubating purified HLA-DP4 in the presence of a labeled peptide and various concentrations of at least one test molecule thereby forming a HLA-DP4/labeled peptide complex and at least one HLA-DP4/test molecule complex, wherein the labeled peptide contains a label and the peptide consists of an amino acid sequence of: SEQ ID NO: 4 or a fragment of SEQ ID NO: 4 which includes at least positions 10 to 18 of SEQ ID NO: 4, SEQ ID NO: 9 or a fragment of SEQ ID NO: 9 which includes at least positions 4 to 12 of SEQ ID NO: 9, SEQ ID NO: 12 or a fragment of SEQ ID NO: 12 which includes at least positions 3 to 11 of SEQ ID NO: 12, SEQ ID NO: 13 or a fragment of SEQ ID NO: 13 which includes at least positions 8 to 16 of SEQ ID NO: 13, SEQ ID NO: 14 or a fragment of SEQ ID NO: 14 which includes at least positions 5 to 13 of SEQ ID NO: 14, SEQ ID NO: 18 or a fragment of SEQ ID NO: 18 which includes at least positions 3 to 11 of SEQ ID NO: 18, or SEQ ID NO: 19 or a fragment of SEQ ID NO: 19 which includes at least positions 6 to 14 or 2 to 10 of SEQ ID NO: 19; (ii) separating the HLA-DP4/labeled peptide complex and the at least one HLA-DP4/test molecule complex formed; (iii) detecting the at least one HLA-DP4/labeled peptide complex by measuring a signal associated with the labeled peptide; and (iv) selecting at least one test molecule that exhibits a binding activity IC