Patent ID: 8609331
Filing Date: 2013-12-17
Classification: C12N,C12Q

Abstract:
1. A method of detecting the presence of an adventitious agent in a composition comprising a virus which virus is not the adventitious agent wherein the non-adventitious virus contains an RNA genome or utilizes an RNA transcript to replicate, comprising: (a) providing a population of indicator cells that expresses a ribozyme, wherein the ribozyme is capable of specifically cleaving the RNA genome or RNA transcript of the non-adventitious virus to inhibit replication or infection of the non-adventitious virus; (b) contacting the indicator cells with the composition under conditions that allow for cleavage of the RNA genome or RNA transcript of the non-adventitious virus by the ribozyme; and (c) determining the effect of the composition on the indicator cells, wherein any pathogenic effect indicates the presence of an adventitious agent in the composition.