Patent ID: 6448382
Filing Date: 2002-09-10
Classification: A61K,A61L,A61P,C07K,C12N

Abstract:
A method for preparing a mixture of intact and single-clipped isoforms of biologically active recombinant PDGF-BB (rPDGF-BB) dimeric protein from a mixture of isoforms of said protein obtained from a cell culture, wherein the rPDGF-B polypeptides in said rPDGF-BB dimeric protein are native-sequence or variant rPDGF-B polypeptides that exhibit at least about 80% sequence identity to native-sequence rPDGF-B polypeptides, said method comprising loading said mixture of isoforms onto a TSK sulfopropyl cation exchange chromatography column, washing said mixture of isoforms from said column with a linear gradient buffer, discarding an eluted fraction comprising a double-clipped isoform, and collecting an eluted fraction comprising intact and single-clipped isoforms.