Patent ID: 8143018
Filing Date: 2012-03-27
Classification: C12Q

Abstract:
1. A method for preparing a measurement sample for detecting live cells of a microorganism in a test sample by flow cytometry, which comprises the following steps: a) treating the test sample with an enzyme comprising lipolytic enzymes and proteases and having an activity of decomposing cells other than those of the microorganism, colloidal particles of proteins or lipids existing in the test sample, and b) treating the test sample with a topoisomerase poison selected from ethidium monoazide, amsacrine, camptothecin, doxorubicin, ellipticine, etoposide, mitoxantrone, saintopin, topotecan and CP-115,953, and/or a DNA gyrase poison selected from ciprofloxacin, ofloxacin, enoxacin, pefloxacin, fleroxacin, norfloxacin, nalidixic acid, oxolinic acid and piromidic acid, and c) treating the test sample treated in steps a) and b) with propidium iodide and SYTO9.