Patent ID: 7851155
Filing Date: 2010-12-14
Classification: C07B,C12Q,C40B,Y10S

Abstract:
1. A method comprising: reacting a first oligomer block, a second oligomer block, and optionally a condensation reagent or reagents under condensation conditions to thereby form a combination oligomer having a linker of at least three atoms in length that is not part of a nucleobase containing backbone subunit of the polymer, wherein said linker covalently links the nucleobase containing backbone subunits of the two component oligomer blocks, such that the combination oligomer hybridizes juxtaposed to a target sequence without a gap or a gap base; wherein, (i) the first and second oligomer blocks are each independently a peptide nucleic acid oligomer; (ii) one, both or all oligomer blocks do not comprise nucleobase protecting groups; and (iii) the combination oligomer forms in the absence of a template; and wherein: the each of the two oligomer blocks comprise at least one label of an energy transfer set of labels wherein the labels of the set are linked to the oligomer blocks at positions that facilitate a change in detectable signal of at least one label when the combination oligomer that is formed by the ligation reaction is hybridized to a target sequence as compared to when the combination oligomer is in a non-hybridized state.