Patent ID: 6335196
Filing Date: 2002-01-01
Classification: C07B,C07K,C12N,C12P,G01R,Y10T

Abstract:
An isotopically substituted nutrient medium for the cultivation of bacterial, yeast, mammalian or insect cell cultures, which comprises all amino acids required for protein biosynthesis, assimilable sources of carbohydrate, essential minerals and growth factors,wherein at least one species of said amino acids in said nutrient medium contains an isotopic substitution; wherein said isotopic substitution is in the backbone structure but not the side chains of said at least one species of amino acid or in hydrogen atoms bonded to the &agr;-carbon of said at least one species of amino acid or both; and wherein said isotopic substitution is selected from 13C; 15N; 2H; 13C and 2H; 15N and 2H; 13C and 15N; and 13C, 15N and 2H; with the proviso that when said at least one species of amino acid is glycine, isotopic substitutions of 2H may occur at either or both of the hydrogen atoms bonded to the &agr;-carbon and with the proviso that essentially none of the isotopic substitutions are present in the side chains of any amino acid.