Patent ID: 9096884
Filing Date: 2015-08-04
Classification: C12Q,G01N

Abstract:
1. A method of detecting NAD-dependent bacterial ligase activity in a sample comprising a mammalian cell comprising: (a) treating the sample under conditions sufficient to lyse bacterial cells; (b) after step (a), treating the sample under high pH conditions that inhibit the mammalian background from ATP-dependent ligase but which do not inhibit bacterial NAD-dependent ligases released during the lysis of step (a); (c) contacting the sample or a portion of the sample with a nucleic acid molecule which acts as a substrate for NAD-dependent ligase activity in the sample, (d) incubating the thus contacted sample under conditions suitable for NAD-dependent ligase activity; and (e) determining the presence, amount, or both of a ligated nucleic acid molecule resulting from the action of the NAD-dependent ligase on the substrate nucleic acid molecule to indicate the presence of NAD-dependent bacterial ligase activity.