Patent ID: 7824609
Filing Date: 2010-11-02
Classification: A61F,A61L

Abstract:
1. In a method of performing a decellularizing treatment of a cell-containing cardiovascular tissue matrix to prepare a bioprosthesis to be coated with endogenic cells from a recipient of the prosthesis, said cardiovascular tissue being of allogenic or xenogenic origin, the improvement consisting of the following steps: 1) providing a treatment apparatus for circulating a treatment medium in contact with said cardiovascular tissue matrix, said treatment apparatus including: 2) fixing said cardiovascular tissue matrix to said holder; 3) contacting said cell-containing cardiovascular tissue matrix with a first medium containing a bile acid by circulating said first medium through said treatment chamber; 4) rinsing said cardiovascular tissue matrix with a second medium by circulating said second medium through said treatment chamber; 5) contacting said cardiovascular tissue matrix with a third medium containing alcohol by circulating said third medium through said treatment chamber; and 6) rinsing said cardiovascular tissue matrix with a fourth medium by circulating said fourth medium through said treatment chamber; each of said media in said steps 4) and 6) being a buffer solution; and said cardiovascular tissue matrix being subjected to mechanical action produced by unidirectional pulsating continuous flow of a respective medium in at least step 6).