Patent ID: 8846878
Filing Date: 2014-09-30
Classification: C07K,C12N

Abstract:
1. A method for recovering nucleic acids and proteins from a biological sample comprising the steps of: (a) providing a biological sample; (b) providing a nucleic acid binding solution; (c) mixing said biological sample with said nucleic acid binding solution; (d) providing a first porous silica compound configured to reversibly bind a nucleic acid; (e) contacting a product of step (c) with said first porous silica compound; (f) separating a fluid product of step (e) from said first porous silica compound; (g) providing a nucleic acid elution solution; (h) contacting said nucleic acid elution solution with said first porous silica compound after step (f) to cause a majority of said nucleic acid bound to said porous silica compound to unbind; (i) collecting said unbound nucleic acid; (j) providing a protein binding solution; (k) mixing said fluid product of step (f) with said protein binding solution; (l) providing a protein binding porous silica compound configured to reversibly bind a protein; (m) contacting a product of step (k) with said protein binding porous silica compound; (n) separating a fluid product of step (m) from said protein binding porous silica compound; (o) providing a protein elution solution; (p) contacting said protein elution solution with said protein binding porous silica compound after step (m) to cause a majority of said protein bound to said protein binding porous silica compound to unbind; and (q) collecting said unbound protein.