Patent ID: 9193793
Filing Date: 2015-11-24
Classification: A61K,A61P,C07K

Abstract:
1. A protein comprising an antigen binding site of an antibody, wherein the antigen binding site of the protein binds to human granulocyte-colony stimulating factor receptor (hG-CSFR) and neutralizes granulocyte-colony stimulating factor (G-CSF) signaling, wherein the protein competitively inhibits the binding of monoclonal antibody C1.2 or monoclonal antibody C1.2 G to one or more of: (i) a polypeptide of SEQ ID NO: 1 in which an alanine is substituted for the lysine at position 167 of SEQ ID NO: 1; and/or (ii) a polypeptide of SEQ ID NO: 1 in which an alanine is substituted for the histidine at position 168 of SEQ ID NO: 1, wherein C1.2 comprises a V H comprising a sequence set forth in SEQ ID NO: 2 and a V L comprising a sequence set forth in SEQ ID NO: 3, and C1.2 G comprises a V H comprising a sequence set forth in SEQ ID NO: 4 and a V L comprising a sequence set forth in SEQ ID NO: 5, wherein the antigen binding site of the protein also binds to the polypeptide at (i) and/or (ii), and wherein the level of binding of the protein to a polypeptide of SEQ ID NO: 1 in which an alanine is substituted for any one of: (a) the arginine at position 287 of SEQ ID NO:1; (b) the histidine at position 237 of SEQ ID NO:1; (c) the methionine at position 198 of SEQ ID NO:1; (d) the tyrosine at position 172 of SEQ ID NO:1; (e) the leucine at position 171 of SEQ ID NO:1; or (f) the leucine at position 111 of SEQ ID NO:1 is lower than the level of binding of the protein to a polypeptide of SEQ ID NO: 1 and wherein the protein comprises a V H and a V L , wherein: (1) the V (2) the V