Patent ID: 6410231
Filing Date: 2002-06-25
Classification: C12Q

Abstract:
A method for detecting multiple SNPs in a population of target polynucleotides in parallel, the method comprising the steps of:(a) combining sample polynucleotides; capture polynucleotides and SNP probes under conditions wherein: a subset of the sample polynucleotides are target polynucleotides, each comprising a different capture region and a different SNP region comprising a corresponding different SNP, the capture polynucleotides are immobilized and arrayed at corresponding discrete elements on a substrate and each capture polynucleotide comprises a sequence which specifically hybridizes to a corresponding different capture region, each SNP probe comprises a sequence complementary to a corresponding different SNP region, the target polynucleotides are immmobilized by hybridizing to the capture polynucleotides whereby each target polynucleotide is immobilized at a corresponding discrete element of the substrate to provide a first discrimination of the sample polynucleotides, and the relative affinity of each SNP probe for the corresponding SNP region is sufficient to provide selective hybridization of the SNP probe to the corresponding SNF region, whereby each SNP probe selectively hybridizes to the corresponding SNP region to provide a second discrimination of the sample polynucleotides; and (b) detecting the presence of each SNP probe on the substrate, wherein the presence of a given SNP probe at a given element indicates the presence of the corresponding SNP in the corresponding target polynucleotide.