Patent ID: 6156557
Filing Date: 2000-12-05
Classification: A61K,C11D,C12N

Abstract:
An alkaline protease which is produced by Streptomyces Sp. TOTO-9305 strain which has been accorded Accession No. FERM P-13640 having the following physical and chemical properties:(a) it possesses non-specific endoproteolytic activity;(b) the optimum pH of the enzyme ranges from 11.0 to 11.5 when using casein as a substrate;(c) it is stable within the pH range of from 1.5 to 12.0 when it is treated at 30.degree. C. for 24 hours;(d) the optimum operating temperature of the enzyme ranges from 70 to 75.degree. C.;(e) the enzyme is stable up to 55.degree. C. in the absence of calcium ions and up to 60.degree. C. in the presence of calcium ions, when it is treated at pH 7.0 for 10 minutes;(f) the enzyme has a molecular weight of about 56,000 (as determined by the SDS-PAGE method);(g) the enzyme has an isoelectric point ranging from about 10 to 10.5 (as determined by the isoelectric focusing method);(h) the enzyme has a specific activity of 214,000 (U/mg protein) when using casein as a substrate and 52,700 (U/mg protein) when using keratin as a substrate; and(i) the activity of the enzyme is not inhibited by PCMB (p-chloromercuribenzoate), iodoacetic acid and EDTA (ethylenediaminetetraacetic acid), but is inhibited by DFP (diisopropylfluorophosphate) and PMSF (phenyl methanesulfonyl fluoride).