Patent ID: 7820796
Filing Date: 2010-10-26
Classification: C07K

Abstract:
1. A method for purification of a Factor VIII polypeptide comprising: (a) adding a mixture containing a Factor VIII polypeptide to be purified to an affinity matrix which binds the Factor VIII polypeptide by hydrophobic and/or electrostatic and/or van der Waals attractions; (b) eluting the Factor VIII polypeptide from the affinity matrix with an elution solution which desorbs the Factor VIII polypeptide; (c) diluting the elution solution about 3-fold to about 5-fold with a solution comprising higher salt concentration than that of the elution solution, resulting in a diluted Factor VIII solution comprising about 7 to about 20 mM of a salt; (d) passing the diluted Factor VIII solution through an ion exchange column which binds the Factor VIII polypeptide, thereby allowing contaminants to pass through the ion exchange column; and (e) eluting the purified factor VIII polypeptide from the ion exchange column.