Patent ID: 6417352
Filing Date: 2002-07-09
Classification: A61P,C07D,C12P

Abstract:
A method for isolating a pharmaceutically acceptable alkali metal salt of clavulanic acid from a fermentation broth comprising clavulanic acid, comprising:a) diluting a fermentation broth comprising clavulanic acid by adding water in a volume equal to between ten percent and seventy percent of the volume of the fermentation broth, resulting in a diluted fermentation broth comprising clavulanic acid; b) filtering the diluted fermentation broth of a), resulting in a filtered fermentation broth comprising clavulanic acid; c) extracting the clavulanic acid to a water immiscible or partly water immiscible solvent, resulting in a solution comprising clavulanic acid; d) adding a solution of a first alkali metal alkylalkanoate to the solution of c), and precipitating an alkali metal salt A of clavulanic acid; e) converting the alkali metal salt A of clavulanic acid to clavulanic acid by adding an inorganic acid; f) extracting the clavulanic acid to a water immiscible or partly water immiscible solvent; and g) adding a second alkali metal alkylalkanoate and precipitating an alkali metal salt B of clavulanic acid.