Patent ID: 8173603
Filing Date: 2012-05-08
Classification: A61P,C07K

Abstract:
1. A method for inhibiting T cell proliferation and/or cytokine production by a T cell comprising contacting a T cell with a BTL-II protein, whereby proliferation of the T cell and/or cytokine production by the T cell is inhibited, and wherein the BTL-II protein is selected from the group consisting of: (a) a BTL-II protein comprising the amino acid sequence of amino acids 29 to 457 of SEQ ID NO:4; (b) a BTL-II protein comprising an amino acid sequence at least 85% identical to amino acids 30 to 358 of SEQ ID NO:10, wherein the identity region of the amino acid sequence aligned with amino acids 30 to 358 of SEQ ID NO:10, is at least 150 amino acids, wherein the amino acid sequence is at least 85% identical to amino acids 127 to 157 of SEQ ID NO:10, wherein the identity region of the amino acid sequence aligned with amino acids 127 to 157 of SEQ ID NO:10, is at least 20 amino acids long, and wherein the BTL-II protein can inhibit the proliferation of T cells induced by an anti-CD3 antibody; (c) a BTL-II protein comprising an amino acid sequence at least 85% identical to amino acids 32 to 358 of SEQ ID NO:10, wherein the identity region of the amino acid sequence aligned with amino acids 32 to 358 of SEQ ID NO:10 is at least 275 amino acids, and wherein the BTL-II protein can inhibit the proliferation of T cells induced by an anti-CD3 antibody; or (d) a BTL-II protein comprising a first amino acid sequence at least 85% identical to amino acids 32 to 358 of SEQ ID NO:10, wherein the identity region of the first amino acid sequence aligned to SEQ ID NO:10 is at least about 175 amino acids long, wherein the first amino acid sequence is not more than 390 amino acids in length, wherein the BTL-II protein can inhibit the proliferation of T cells induced by an anti-CD3 antibody, wherein the first amino acid sequence is not at least 85% identical to amino acids 148 to 232 of SEQ ID NO:4 with an identity region of the first amino acid sequence aligned to amino acids 148 to 232 of SEQ ID NO:4 of at least about 40 amino acids, and wherein the protein does not comprise a second amino acid sequence that is at least 85% identical to amino acids 148 to 232 of SEQ ID NO:4 with an identity region of the second amino acid sequence aligned to amino acids 148 to 232 of SEQ ID NO:4 of at least about 40 amino acids.