Patent ID: 7795210
Filing Date: 2010-09-14
Classification: C07K

Abstract:
1. A cell-free, in vitro method of glycosylating a peptide with a glycosyl residue which is a member selected from N-acetylgalactosamine, galactose, mannose, N-acetylglucosamine, glucose, fucose and xylose, said glycosyl residue covalently attached to an amino acid of said peptide which is a hydroxyamino acid, said method comprising: (a) contacting said peptide with a donor of said glycosyl residue and a glycosyltransferase for which said donor is a substrate under conditions appropriate to transfer said glycosyl residue from said donor to said hydroxyamino acid, wherein said donor is a nucleotide sugar conjugated to a poly(alkylene oxide), thereby glycosylating said peptide.