Patent ID: 6900013
Filing Date: 2005-05-31
Classification: C07H,C12Q

Abstract:
1. A method of detecting a mutation or single nucleotide polymorphism (SNP), comprising: a) contacting one or more probes that comprise DNA with a survey population of RNA molecules under conditions that promote hybridization between complementary nucleic acid molecules to generate a probe-survey population mixture of nucleic acid molecules that comprises at least one of said one or more probes hybridized to one or more survey population RNA molecules, wherein said one or more probes are at least partially complementary to one or more RNA molecules known to be or suspected of being present in the survey population, further wherein terminal nucleotide of said one or more probes is a SNP or mutation; b) treating said probe-survey population mixture of nucleic acid molecules with at least one nuclease that digests single-stranded DNA molecules, such that non-base-paired deoxynucleotides are digested, to generate a population of nucleic acid molecules comprising one or more nuclease-protected probes; c) contacting said population of nucleic acid molecules comprising one or more nuclease-protected probes with a solid support that comprises one or more attached nucleic acid molecules under conditions that promote hybridization between complementary nucleic acid molecules, wherein said one or more attached nucleic acid molecules are at least partially complementary to said one or more probes, to generate one or more attached nucleic acid molecule/nuclease-protected probe complexes; and d) detecting at least one of said one or more attached nucleic acid molecule/probe complexes to detect said SNP or mutation.