Patent ID: 6897028
Filing Date: 2005-05-24
Classification: C12N,C40B,G01N

Abstract:
1. A process for the identification of a protein which binds to a ligand, wherein the ligand is an organic molecule conjugated with a plating agent, the ligand having a molecular weight which is less than 5.000 Daltons and being other than a nucleic acid, peptide or protein, the process comprising: screening the ligand against a peptide or protein library wherein the peptide or protein members of the library are selected from the group consisting of expression products of a cDNA library derived from a cell and fragments of those expression products, and wherein the library comprises genetic packages which physically link each peptide or protein member of the library to a nucleic acid polymer which encodes that member, separating form the library members of the library which have an affinity for the ligand which is greater than the affinity possessed by other members of the library for the ligand, determining the nucleic acid sequence which encode the members which have been separated from the library and translating these nucleic acid sequences into peptide sequences, and identifying, protein(s) which contain a portion of the translated peptide sequences or which correspond to consensus peptide sequences derived from statistical analysis of said translated library member peptide sequences.