Patent ID: 8652817
Filing Date: 2014-02-18
Classification: C12N,C12P,Y02E

Abstract:
1. A recombinant host cell suitable for degrading a saccharide comprising: (a) a heterologous polynucleotide sequence that codes for an enzyme that converts sugars to ethanol, wherein said host cell expresses said heterologous polynucleotide sequence at a sufficient functional level so as to facilitate production of ethanol as a primary fermentation product by said cell; and (b) a mutation in at least one polynucleotide sequence that codes for a protein in a metabolic pathway in said cell that produces a product other than ethanol from sugars, wherein said mutation results in increased ethanol production by said cell, as compared to ethanol production by the cell in the absence of said mutation; wherein the recombinant host cell is a Gram-negative bacterium selected from the group consisting of