Patent ID: 6110699
Filing Date: 2000-08-29
Classification: B01D,C12P

Abstract:
A process for obtaining a 6-amino penicillanic and phenylacetic acid product from a culture broth in which a penicillin-producing microorganism has undergone fermentation to form penicillin having a ring structure containing a side chain linked to an amine group in position 6 of the ring thereof, said culture broth comprising the biomass of the microorganism and products of the fermentation, including the penicillin, consisting essentially of the following successive steps:(a) purifying the penicillin containing culture broth by (i) separating the biomass or a portion thereof from the culture broth and then (ii) ultrafiltrating the remaining culture broth with at least one filter having a molecular cut-off size in a range of from 20,000 to 2,000 Dalton to separate the penicillin and other products of the fermentation that pass through the at least one filter from the remainder of the products of the fermentation and from any remaining biomass, said ultrafiltration process forming an ultrafiltrate containing the penicillin and the other products that pass through the at least one filter,(b) treating the ultrafiltrate with an enzyme that catalyzes the removal of the side chain linked to the amine group in position 6 of the ring of the penicillin to form an enzymatically converted ultrafiltrate comprising 6-aminopenicillanic acid and phenylacetic acid;(c) separating the 6-aminopenicillanic acid and phenylacetic acid by fractionated sequential separation using exchange resins; and(d) recovering the 6-aminopenicillanic acid, the phenylacetic acid or both.