Patent ID: 9206431
Filing Date: 2015-12-08
Classification: C07K,C12N

Abstract:
1. A kit comprising 2-6 expression vectors, each of which includes: a first nucleic acid encoding a protein tag, and a second nucleic acid containing (i) a nucleotide sequence that encodes a protease recognition site and (ii) one or two restriction sites identical in all of the 2-6 expression vectors for cloning a target gene that encodes a target polypeptide, wherein each of the expression vectors allows in a host cell production of a fusion protein including the protein tag, the protease recognition site, and the target polypeptide, which is separable from the protein tag via protease cleavage at the protease recognition site; and at least two of the expression vectors allow protein production in two different host cells, which are selected from the group consisting of an wherein the second nucleic acid includes the nucleotide sequence of GAGAACCTGTACGTACAG (SEQ ID NO: 1), which encodes a TEV protease recognition site and includes an SnaB I site used for cloning the target gene.