Patent ID: 8318474
Filing Date: 2012-11-27
Classification: C07K,C12N,C12P

Abstract:
1. A yeast cell, comprising: (i) a mutation in the GAL2 gene that removes positive feedback control of the expression of the GAL2 gene, wherein said mutation comprises a loss-of-function mutation in GAL2 promoter, or comprises a GAL2 deletion; and (ii) a nucleic acid encoding a protein of interest, an RNA, or a polypeptide that regulates the production of a compound of interest, said nucleic acid being operably linked to a GAL-responsive promoter, wherein the GAL-responsive promoter is a heterologous promoter or a promoter that is not a naturally-occurring yeast promoter, wherein expression of the protein of interest, the RNA, or the polypeptide has a linear response to galactose or galactose analog that binds to GAL3.