Patent ID: 7019195
Filing Date: 2006-03-28
Classification: C12N

Abstract:
1. A method for conferring resistance or tolerance to more than one virus selected from the group consisting of a furovirus, potyvirus, tospovirus, and cucomovirus upon a plant cell comprising the step of: introducing into a plant cell more than one pair of DNA sequences wherein for each pair, the first DNA sequence encodes a portion of the viral genome of a furovirus, potyvirus, tospovirus, or cucomovirus, and the second DNA sequence of the pair encodes a sequence that is antisense to the first DNA sequence, such that the RNA sequences encoded by the first and second DNA sequences of a pair form a double-stranded RNA molecule when expressed in said plant cell, resulting upon viral infection, in reduced expression of the portion of the viral genome from which the DNA sequences of that pair were derived, and wherein at least two of the introduced pairs of DNA sequences were derived from different viruses of said group, resulting in resistance or tolerance of said plant cell to more than one virus of said group.