Patent ID: 8217153
Filing Date: 2012-07-10
Classification: C07K

Abstract:
1. A method for purifying protein-A-selected target molecules from a complex solution by affinity column chromatography, the method comprising: (a) loading the complex solution onto a protein A-based affinity-column-chromatography column; (b) adsorbing the protein-A-selected target molecules to an affinity-column-chromatography matrix having protein-A bound thereto within the protein A affinity-column-chromatography column; (c) the chromatography column with a wash solution comprising between 5% and 60% by volume of one or more of the organic solvents methyl propanediol, 2-ethyl-1,3-hexanediol, benzyl alcohol, ethylene glycol, 1,3-butanediol, 1,6-hexanediol, pyrocatechol, triethylene glycol, 1,4-butanediol, 1,2-octanediol, resorcinol, glycerol, 1,2,4-butanetriol, 1,8-octanediol, hydroquinone, 4-hydroxymethyl-1,3-dioxolane, 5-hydroxy-1,3-dioxane, tributyl phosphate, and 1,9-nonaediol, the wash solution maintained at a pH of between 6.5 and 8.0; and (d) eluting the target antibody molecules by using an eluant.