Patent ID: 6506558
Filing Date: 2003-01-14
Classification: B01J,B82Y,C07B,C07C,C07D,C07H,C07K,C12Q,C40B,G01N,G03F,G11C,Y02P

Abstract:
An ordered method for forming a plurality of different polypeptides or nucleic acids occupying known locations on a substrate by sequential addition of nucleosides, nucleotides or amino acids to said substrate, comprising:(a) providing a substrate having a surface with a localized area occupied by a protected nucleoside, nucleotide or amino acid; (b) deprotecting the protected nucleoside, nucleotide or amino acid in a fraction of the localized area to produce a deprotected nucleoside, nucleotide or amino acid; (c) reacting the deprotected nucleoside, nucleotide or amino acid with a protected nucleoside, nucleotide or amino acid, whereby the protected nucleoside, nucleotide or amino acid attaches to the deprotected nucleoside, nucleotide or amino acid to produce a protected nucleic acid or polypeptide; and (d) repeating (b) and (c) at least once wherein the fraction in step (b) is a fraction of a fraction in a previous deprotecting step (b) to produce an array of different nucleic acids or polypeptides occupying different fractions at known locations within the localized area.