Patent ID: 6872537
Filing Date: 2005-03-29
Classification: C12N,C12Q,G01N

Abstract:
1. A method of identifying a test agent that modulates at least one activity selected from the group consisting of microtubule depolymerization, microtubule polymerization and microtubule severing, said method comprising the steps of: (i) contacting a polymerized microtubule with at least one protein selected from the group consisting of a microtubule severing protein and a microtubule depolymerizing protein, in the presence of ATP or GTP, and said test agent; and (ii) detecting the formation of at least one product selected from the group consisting of tubulin monomers, dimers and oligomers, wherein the formation of said tubulin monomers, dimers, or oligomers indicates that said test agent modulates microtubule depolymerization.