Patent ID: 6180844
Filing Date: 2001-01-30
Classification: C12Q

Abstract:
A composition for forming fluorescent dyes in oxidative environments, which comprises a fluorescence-generating substrate or fluorescent dye precursor of formula (I): ##STR19##wherein R.sup.1 and R.sup.2 are each independently selected from the group consisting of a hydrogen atom, or an electron-donating substituent;wherein the electron-donating substituent is selected from the group consisting of a lower alkyl group, a lower alkoxy group, a hydroxyl group, an alkylcarbonyloxy group, an amino group di-substituted with a lower alkyl group, or an aryl group;and, wherein L is selected from the group consisting of --NHCO-- and, --CONH--;and wherein Y is selected from the group consisting of -alkylene-COOH and formula (II): ##STR20##wherein R.sup.3, R.sup.4 and R.sup.5 are each independently selected from the group consisting of a hydrogen atom, a carboxyl group, a 5- or 6-membered hydrocarbon ring fused to the aromatic ring formed by any two of R.sup.3, R.sup.4 and R.sup.5, together with the adjacent carbon atoms to which they bond, and an electron-donating substituent;wherein the electron-donating substituent is selected from the group consisting of a lower alkyl group, a lower alkoxy group, a hydroxyl group, an alkylcarbonyloxy group, an amino group di-substituted with a lower alkyl group, and an aryl group;and, wherein, -alkylene- is selected from the group of linear and branched C.sub.1-6 alkylene groups;and, wherein a is 1.