Patent ID: 8143485
Filing Date: 2012-03-27
Classification: C12N

Abstract:
1. An isolated nucleic acid molecule encoding a FAD2 protein having reduced desaturase activity, wherein the molecule comprises a nucleotide sequence selected from the group consisting of: (a) the nucleotide sequence of SEQ ID NO: 1 wherein the nucleotide at position 215 is deleted; (b) the nucleotide sequence of SEQ ID NO: 5 wherein the nucleotide at position 215 is deleted; (c) the nucleotide sequence of SEQ ID NO: 3, its complementary form, or the RNA form thereof; (d) a nucleotide sequence having at least 95% identity with the nucleotide sequence of SEQ ID NO: 1 wherein the nucleotide corresponding to position 215 is deleted; (e) a nucleotide sequence having at least 95% identity with the nucleotide sequence of SEQ ID NO: 5 wherein the nucleotide corresponding to position 215 is deleted; (f) a nucleotide sequence fragment comprising at least 10 contiguous nucleotides of an isolated nucleic acid molecule of (a), wherein the fragment comprises a mutated codon resulting from the deletion at position 215; and (g) a nucleotide sequence fragment comprising at least 10 contiguous nucleotides of an isolated nucleic acid molecule of (b), wherein the fragment comprises a mutated codon resulting from the deletion at position 215.