Patent ID: 7820162
Filing Date: 2010-10-26
Classification: A61K,A61P,C07K,C12N,C12Y

Abstract:
1. A method of synthesizing a chimeric monomer-dimer hybrid comprising a) recombinantly expressing a fusion protein comprising a portion of an immunoglobulin containing an Fc neonatal receptor (FcRn) binding site and a splicing protein capable of forming a thioester intermediate on the C terminus of the portion containing the FcRn binding site; b) adding a thiol cofactor to the fusion protein of a); c) adding at least one biologically active molecule having an N terminal cysteine, thereby linking the biologically active molecule with the C terminus of the portion containing the FcRn binding site; and d) adding a second polypeptide containing an FcRn binding site, thereby forming a chimeric monomer-dimer hybrid; wherein the biologically active molecular is a polypeptide or a nucleic acid molecule.