Patent ID: 6239159
Filing Date: 2001-05-29
Classification: C07H

Abstract:
A nucleoside analogue of the formula: ##STR19##where Z is O, S, Se, SO NR.sup.9 or CH.sub.2,R.sup.1, R.sup.2, R.sup.3 and R.sup.4 are the same or different and each is H, OH, F, NH.sub.2, N.sub.3, O-hydrocarbyl or a reporter moiety,R.sup.5 is OH or mono-, di- or tri-phosphate or -thiophosphate or corresponding boranophosphate,or one of R.sup.2 and R.sup.5 is a phosphoramidite or other group for incorporation in a polynucleotide chain,and M is: ##STR20##where X.sup.1, X.sup.2 and X.sup.3 are the same or different and each is C or N, when X.sup.3 is N, there is no R.sup.7 group,R.sup.6 and R.sup.7 are the same or different and each is H, NO.sub.2, CO, COR.sup.8, OR.sup.8, CN, O, CON(R.sup.8).sub.2, COOR.sup.8, SO.sub.2 R.sup.8, SO.sub.3 R.sup.8, SR.sup.8, NHCHO, (CH.sub.2).sub.n N(R.sup.8).sub.2, halogen, or a reporter moiety,each of R.sup.8 and R.sup.9 is H or hydrocarbyl or a reporter moiety,and n is 0, 1, 2, 3 or 4,provided that when R.sup.5 is not triphosphate, then the nucleoside analogue comprises a reporter moietyand provided that, when M is benzimidazolyl and a reporter moiety is absent, the M has the structure: ##STR21##and R.sup.6 is not H,and further provided that when M is: ##STR22##then R.sup.5 is not OH.