Patent ID: 9057056
Filing Date: 2015-06-16
Classification: C12N

Abstract:
1. A method for transferring a gene of interest into a target cell, the method comprising: (i) increasing the expression or activity of N-acetylglucosaminyltransferase III in a human retrovirus producer cell in vitro; (ii) culturing said retrovirus producer cell in vitro, wherein said producer cell has a gag-pol gene and an env gene derived from a retrovirus, and wherein said producer cell is transformed with a recombinant retrovirus or a recombinant retrovirus plasmid vector, wherein said retrovirus or said plasmid vector comprises said gene of interest; (iii) collecting a culture supernatant from the culture in (ii) to obtain a retrovirus comprising said gene of interest; and (iv) infecting a target cell with the retrovirus of (iii) in the presence of a fibronectin fragment having both a cell adhesion domain and a heparin-binding domain in vitro, wherein the cell adhesion domain comprises a very late antigen (VLA)-5-binding domain and a VLA-4-binding domain.