Patent ID: 6133030
Filing Date: 2000-10-17
Classification: B82Y,C12M,C12N

Abstract:
A method for producing a micropatterned co-culture containing at least two cell types, the method comprising:i) providing a substrate coated with a cell-binding protein which defines a micropattern on the substrate;ii) contacting the cell-binding protein with cells of a first cell type suspended in a first cell medium under conditions such that cells of the first cell type bind the cell-binding protein, thereby producing a micropatterned cell-coated substrate; andiii) contacting the micropatterned cell-coated substrate with cells of a second cell type suspended in a second cell medium under conditions such that cells of the second cell type bind the substrate, thereby producing the micropatterned co-culture, wherein one of the cell media is a selective medium that lacks serum and attachment factors and/or includes a non-adhesive factor to inhibit attachment, and one of the cell media is an attachment medium that contains an effective amount of serum and/or at least one attachment factor.