Patent ID: 7097976
Filing Date: 2006-08-29
Classification: C12N,C12Q

Abstract:
1. A method of identifying at least one heterozygous gene showing monoallelic expression in an individual the method comprising: (a) providing a genomic DNA sample from an individual; (b) providing a nucleic acid array comprising probes designed to interrogate the genotype of a plurality of single nucleotide polymorphisms; (c) hybridizing the genomic DNA sample to a first copy of the array; (d) generating a hybridization pattern resulting from said hybridization; (e) analyzing the hybridization pattern to determine the identity of the alleles present for at least one polymorphism in said plurality of single nucleotide polymorphisms; (f) identifying at least one polymorphism in said plurality of single nucleotide polymorphisms that is heterozygous in the individual; (g) isolating an RNA sample from the individual; (h) hybridizing the RNA sample to a second copy of said array and generating a second hybridization pattern; and (i) identifying at least one polymorphism in said plurality of single nucleotide polymorphisms that is heterozygous in the genomic DNA sample and homozygous in the RNA sample.