Patent ID: 6740484
Filing Date: 2004-05-25
Classification: A01N,A61K

Abstract:
A method for vitrification of a natural or engineered tissue or organ, comprising:immersing the tissue or organ in a series of solutions having increasing concentrations of cryoprotectant to achieve a cryoprotectant concentration sufficient for vitrification, each solution of said series of solutions having a temperature above âˆ’15Â° C.; under physiological pressure, cooling the tissue or organ in a solution having said cryoprotectant concentration sufficient for vitrification at an average rate of from 2.5 to 80Â° C. per minute from a temperature above âˆ’15Â° C. to a temperature between âˆ’80Â° C. and the glass transition temperature; and further cooling the tissue or organ at an average rate less than 30Â° C. per minute from a temperature that is between âˆ’80Â° C. and the glass transition temperature to a temperature below the glass transition temperature to vitrify the tissue or organ, wherein cooling the tissue or organ from a temperature that is between âˆ’80Â° C. and the glass transition temperature to a temperature below the glass transition temperature is performed at a slower rate than the rate for cooling the tissue or organ from a temperature above âˆ’15Â° C. to a temperature between âˆ’80Â° C. and the glass transition temperature.