Patent ID: 8053419
Filing Date: 2011-11-08
Classification: C12N

Abstract:
1. A double-stranded DNA construct comprising: a first structural gene sequence whose nucleotide sequence is identical to the nucleotide sequence of a region of a target gene in an animal cell; a second structural gene sequence identical in sequence to, and in an inverted orientation relative to, the first structural gene sequence such that a repeating sequence which is identical to the region of the target gene is resent in the DNA construct; a stuffer fragment which consists of nucleotides other than the nucleotides of the repeating sequence, and which separates and links the first and second structural gene sequences; a promoter operable in the animal cell; and a transcription termination sequence active in the animal cell, wherein the first structural gene sequence, the stuffer fragment and the second structural gene sequence are all operably connected to the promoter and the transcription termination sequence.