Patent ID: 6830889
Filing Date: 2004-12-14
Classification: C07D,C12Q,Y10S,Y10T

Abstract:
A method for detecting DNA in a sample comprising carrying out the following processes defined in steps (1) and (2) or in steps (3) or (4):(1) hybridizing to the DNA contained in the sample a biotin-modified nucleic acid probe and a nucleic acid probe labeled with an organic cyanine dye which is sensitized to luminescence by fluorescent resonance energy transfer, and (2) binding streptavidin labeled with 4,4â€²-bis (1â€³,1â€³,2â€³,2â€³,3â€³,3â€³-heptafluoro-4â€³,6â€³-hexanedione-6â€³-yl)-chlorosulfo-o-terphenyl (BHHCT)/Eu3+ complex to the biotin in the DNA duplex obtained by above (1), or (3) binding streptavidin labeled with 4,4â€³-bis-(1â€³,1â€³,2â€³,2â€³,3â€³,3â€³-heptafluoro-4â€³,6â€³-hexanedione-6â€³-yl)-chlorosulfo-o-terphenyl (BHHCT)/Eu3+ complex to the biotin in a biotin-modified nucleic acid probe, and (4) hybridizing to the DNA contained in the sample the probe obtained by above (3) and a nucleic acid probe labeled with an organic cyanine dye which is sensitized to luminescencence by fluorescent resonance energy transfer; and detecting and measuring the fluorescent resonance energy transfer luminescence spectrum of the organic cyanine dye.