Patent ID: 6017761
Filing Date: 2000-01-25
Classification: A61K,C12N

Abstract:
A method for obtaining a recombinant retroviral packaging cell comprising:a. isolating a retroviral nucleic acid sequence encoding a minimal gag-pol open reading frame (ORF), said nucleic acid sequence having no flanking sequences of the gag-pol ORF, and inserting said nucleic acid sequence into a first expression plasmid;b. isolating a retroviral nucleic acid sequence encoding a minimal env ORF, said nucleic acid sequence having no flanking sequences of the env ORF, and inserting said nucleic acid sequence into a second expression plasmid,c. obtaining a eukaryotic cell free of an endogenous nucleic acid sequence which encodes the gag-pol ORF or the env ORF and which is derived from the retrovirus from which the minimal gag-pol ORF or env ORF is isolated; andd. introducing the first and second expression plasmids into the eukaryotic cell and expressing the nucleic acids encoding the minimal gag-pol ORF and env ORF to produce Gag, Pol and Env proteins, thereby producing the recombinant retroviral packaging cell.