Patent ID: 6699721
Filing Date: 2004-03-02
Classification: G01N,Y10T

Abstract:
In the process of laser capture microdissection including:providing a specimen having a matrix of cells for examination; providing a low temperature melt film for laser capture microdissection; overlaying and contacting the specimen with the low temperature melt film; visualizing the specimen to determine a cell area in the matrix of cells for microdissection; heating the film with a laser overlying the cell area to cause the film to melt and locally adhered to the cell area; removing the film from the specimen to cause a locally adhered cell area to be removed with the film; the improvement comprising the step of: placing a specimen non adhering coating on the low temperature melt film, the specimen non adhering coating exposed to and in contact with the sample, the coating being between 0.1% and 5% of the total thickness of the film whereby when the heating of the film overlying the cell area causes melting of the film to occur, melting occurs through the specimen non adhering coating.