Patent ID: 9005896
Filing Date: 2015-04-14
Classification: C12Q

Abstract:
1. A method for amplifying a target nucleic acid comprising the steps of: a) providing a support with a plurality of a nucleic acid capture probe, wherein said plurality of probes is immobilized to the support via the 5′ end of the nucleic acid, b) hybridizing a target nucleic acid to the capture nucleic acid probe to form a probe/target complex, c) extending the capture probe nucleic acid with a polymerase wherein the target nucleic acid is used as template for the extension of the capture probe nucleic acid, d) ligating the extended target/probe double stranded nucleic acid to a hairpin nucleic acid wherein the 5′ end of the hairpin nucleic acid is ligated to 3′ end of the extended probe and wherein the 3′ end of the hairpin nucleic acid is ligated to the 5′ end of the target nucleic acid, e) allowing the 3′ end of the target nucleic acid to bind to a further probe of said plurality of nucleic acid capture probes on said support, f) extending the 3′ end of said further probe by a polymerase, g) amplifying the nucleic acid obtained in step f) by repeating steps e) and f).