Patent ID: 6150328
Filing Date: 2000-11-21
Classification: A61K,A61L,C07K,Y10S

Abstract:
A method for induction selected from the group consisting of the induction of bone formation, cartilage formation and the formation of the combination thereof in a patient comprising administering an amount of a purified BMP-2 protein effective for inducing said formation of bone, cartilage and the combination thereof said BMP-2 protein produced by the steps of:(a) culturing under suitable conditions a cell transformed with a DNA sequence selected from the group consisting of(i) a cDNA comprising the nucleotide sequence from nucleotide #356 to #1543 of SEQ ID NO:3 encoding a subunit of BMP-2; and(ii) DNA sequences comprising a nucleotide sequence which encodes a subunit having the 98 amino acid sequence from amino acid #17 to amino acid #114 of SEQ ID NO: 4said DNA sequence in operative association with an expression control sequence therefor(b) recovering and purifying from said culture medium a protein wherein each subunit comprises the 98 amino acid sequence from amino acid #17 to amino acid #114 of SEQ ID NO: 4 said protein having induction activity selected from the group consisting of bone, cartilage and the combination thereof.