Patent ID: 7989396
Filing Date: 2011-08-02
Classification: C40B,G01N

Abstract:
1. A method for preparing a substrate for use in a biosensor, comprising: (a) providing a substrate surface which is reactive with an amine; (b) coating the substrate surface with a polyamine to form a polyamine covalently linked to the substrate; then (c) coating the polyamine covalently linked to the substrate with a polyacid anhydride having multiple acid anhydride groups to thereby form a layer of polyacid anhydride that binds to the polyamine covalently linked to the substrate with one portion of the multiple acid anhydride groups and retains another portion of the multiple acid anhydride groups for linking to amine groups on biomolecules, said polyacid anhydride coating comprising an at least partially hydrolyzed polyacid; then (d) dehydrating partially hydrolyzed polyacid to form a corresponding polyanhydride for linking to said amine groups on biomolecules.