Patent ID: 8735656
Filing Date: 2014-05-27
Classification: C07K,C12N,C12P

Abstract:
1. A method for producing a secretory glycoprotein having a human-type sugar chain from a transgenic plant cell, the method comprising: providing a transgenic plant cell that comprises a first heterologous nucleic acid sequence encoding a β1,4-galactosyltransferase and a second heterologous nucleic acid sequence encoding a glycoprotein that is heterologous to the plant cell, wherein in the transgenic plant cell, the expression of an endogenous β1,2-xylosyltransferase, α1,3-fucosyltransferase, or both is inhibited or a gene encoding the endogenous β1,2-xylosyltransferase or α1,3-focosyltransferase is mutated; and culturing the transgenic plant cell in a culture medium to allow production and secretion of the glycoprotein, which contains an N-glycan having a galactose residue linked to a non-reducing terminal acetylglucosamine residue; and collecting the culture medium for isolation of the glycoprotein.