Patent ID: 6541438
Filing Date: 2003-04-01
Classification: C11D

Abstract:
A modified enzyme comprising a catalytically active amino acid sequence of a cellulolytic enzyme linked to an amino acid sequence comprising a cellulose binding domain having a relative binding constant for binding to amorphous cellulose of greater than 2.4 l/gcellulose; wherein said modified enzyme comprises a linking region between said catalytically active amino acid sequence of a cellulolytic enzyme and said amino acid sequence comprising a cellulose binding domain; further wherein said linking region is selected from the group consisting of: Humicola insolens family 45 cellulase linker, Nifa gene of Klebsiella pneumoniae-CiP linker, E. coli OmpA gene-CiP linker, E3 cellulase Thermomonospora fusca linker, CenA cellulase linker, PEG(NPC)2 linker, (NH2)2-PEG, t-BOC-NH-PEG-NH2 linker, MAL-PEG-NHS linker, VS-PEG-NHS linker and mixtures thereof; further wherein said amino acid sequence comprising a cellulose binding domain is derived from an organism selected from the group consisting of: Cellulomonas fimi, Clostridium cellulolyticum, Myxoccus xanthus, Streptomyces reticuli, Clostridium stecorarium (NCIMB11754), Thermomonospora fusca and mixtures thereof.