Patent ID: 6320660
Filing Date: 2001-11-20
Classification: G01N

Abstract:
A sieving apparatus for a bio-chip, which has at least one sample to be sieved, the apparatus comprising:a light source to emit a light beam;a holographic optical element (HOE) unit, used to receive the light beam from the light source and diffract the light beam to form a zeroth order beam and a first order beam, wherein the zeroth order beam remains on an optical path without deflection, and the first order beam is deflected away the optical axis abutting the zeroth order beam;a beam splitter, receiving the zeroth order beam and the first order beam from the HOE unit;an objective lens associating with the beam splitter, wherein the beam splitter also leads the zeroth order beam and the first order beam to the objective lens, and the objective lens refracts the zeroth order beam and the first order beam to the bio-chip at one of the at least one sample, whereby the first order beam is incident on the one of the at least one sample from side by an incident angle, so as to generate a fluorescent light if the one of the at least one sample contains a specific component, wherein the bio-chip also reflects the zeroth order beam;a filter, used to receive and filter the fluorescent light and the zeroth order beam from the bio-chip, so that the fluorescent can pass; anda signal sensor, used to receive the fluorescent light passing the filter, so as to determine whether or not the one of the at least one sample contains the specific component.