Patent ID: 7449558
Filing Date: 2008-11-11
Classification: G01N

Abstract:
1. A method for labelling a protein in a cell, comprising introducing a detectable probe having a thioester group into a cell expressing a protein that is proteolytically processed to expose an N-terminal cysteine; and washing the cell; wherein the probe is acetic acid 4-[(benzylsulfanylcarbonylmethyl-carbamoyl)-methyl]-2-oxo-2H-chromen-7-yl ester; N-benzylsulfanylcarbonylmethyl-6-(3,6-dihydroxy-3H-xanthen-9-yl)-isophthalamic acid; amino thioacetic acid S-benzyl ester of 5-carboxy-tetramethylrhodamine; amino thioacetic acid S-benzyl ester of acetoxynapthofluorescein; [5-(2-oxo-hexahydro-thieno[3,4-d]imidazol-6-yl)-pentanoylamino]-thioacetic acid S-benzyl ester; (4-benzoyl-benzoylamino)-thioacetic acid S-benzyl ester; or amino thioacetic acid S-benzyl ester of caged fluorescein, and is cell-permeable.