Patent ID: 7987056
Filing Date: 2011-07-26
Classification: C07K,C12N

Abstract:
1. A method comprising: a) obtaining multiple libraries of DNA molecules, each said library comprising clones and each said clone comprising a vector DNA sequence and an insert DNA sequence, wherein the insert DNA sequences comprise DNA obtained from a selected microorganism genome, and wherein the insert DNA sequences of each of any single library are of a same defined size and the defined size of each library is different from the defined size of any other said library; b) introducing each library of DNA molecules, obtained in step a), into a respective population of microorganism cells to obtain multiple populations of microorganism cells; c) exposing the multiple populations of microorganism cells, obtained in step b), to a condition that alters the distribution of the microorganism cell populations and the clones therein; d) for each of a plurality of positions along the selected genome, measuring, in the altered populations of microorganism cells obtained in step c), the amount of DNA of the insert sequences that correspond to each of the plurality of positions; and e) determining, from the measurements of step d), the relative amount of DNA of each insert size that corresponds to each of the plurality of genomic positions.