Patent ID: 6558941
Filing Date: 2003-05-06
Classification: C12N,C12P,G01N

Abstract:
A method of preparing a cell culture medium containing a lipophilic substance promoting the growth of cells, said method comprising the steps of:(a) preparing a dispersion comprising a nanoemulsion; and (b) adding said dispersion to said cell culture medium; said nanoemulsion including; a lipophilic substance promoting the growth of cells and an oily component consisting of droplets dispersed in an aqueous phase, the oily droplets containing the lipophilic substance, the surface of the oily droplets comprising a non-toxic amphoteric emulsifier, the oily droplets having a diameter of less than 100 nm, and the nanoemulsion comprising from 0.65 to 0.75 parts by weight of amphoteric emulsifier for one part by weight of the oily component.