Patent ID: 6221616
Filing Date: 2001-04-24
Classification: A61M,A61P,C07K

Abstract:
A process for determining whether a chemical compound is a human Melanin-concentrating hormone 1 (MCH1) receptor antagonist which comprises contacting cells transfected with and expressing DNA encoding the human MCH1 receptor with the compound in the presence of a known human MCH1 receptor agonist, under conditions permitting the activation of the human MCH1 receptor, and detecting human MCH1 receptor activity, wherein a decrease in human MCH1 receptor activity in the presence of both the compound and the known agonist relative to the activity of the human MCH1 receptor in the presence of the known agonist alone indicates that the compound is a human MCH1 receptor antagonist, wherein the DNA encoding the human MCH1 receptor comprises the sequence shown in FIG. 1 (SEQ ID NO: 1) or contained in plasmid pEXJ.HR-TL231 (ATCC Accession No. 203197), the known human MCH1 receptor agonist is Melanin-concentrating hormone (MCH) or an analog of MCH, and the cells do not express the MCH1 receptor prior to transfecting them.