Patent ID: 7608437
Filing Date: 2009-10-27
Classification: C12N,C12P

Abstract:
1. A method of producing L-glutamic acid, comprising: culturing a coryneform bacterium expressing enzymes encoded by coryneform bacteria glutamic acid biosyntesizing genes, in a medium for a time and under conditions suitable to produce and accumulate said L-glutamic acid in the medium, and collecting said L-glutamic acid from the medium; wherein the glutamic acid biosynthesizing genes are located on the chromosome of the coryneform bacterium and the enzymes are glutamate dehydrogenase and citrate synthase, wherein said glutamic acid biosynthesizing genes comprise a DNA sequence situated at about position −10 from the transcription start site of the glutamic acid biosynthesizing genes, wherein said DNA sequence is TATAAT, wherein said gene which encodes glutamate dehydrogenase further comprises TTGTCA situated at about position −35 from the transcription start site of the gene, wherein said L-glutamic acid is produced at a level greater than the L-glutamic acid produced by the corresponding wild-type coryneform bacterium.