Patent ID: 6420113
Filing Date: 2002-07-16
Classification: C07K,C12N,C40B,G01N

Abstract:
A method of producing a chimeric antibody library having affinity for a target, comprising:providing a library of replicable genetic packages, wherein a member comprises a replicable genetic package capable of displaying an antibody chain encoded by a genome of the package and the antibody chain varying between members, and wherein the library of replicable genetic packages has been selected from a primary library of replicable genetic packages by affinity selection to a target; subcloning a mixed population of DNA molecules encoding at least four different antibody chains of the library of replicable genetic packages into multiple copies of an expression vector to produce modified forms of the expression vector; and introducing the modified forms of the expression vector into a host and expressing the antibody chains as chimeric antibody chains in the host, wherein a library of at least four different chimeric antibody chains are expressed, at least 90% of modified forms of the expression vector encode chimeric antibody chains having specific affinity for the target and no modified form of the expression vector constitutes more than 50% of the total forms.