Patent ID: 6770277
Filing Date: 2004-08-03
Classification: C07K,C12N,C12Q,G01N

Abstract:
An antibody specifically binding a Streptococcus pyogenes DNase B enzyme produced by the process of:(a) culturing a bacterial host cell transformed with an expression vector comprising a DNA sequence that is substantially purified DNA comprising DNA encoding an amino acid sequence selected from the group consisting of the amino acid sequence of: (1) Streptococcus pyogenes DNase B enzyme as shown in FIG. 4 (SEQ ID NO: 9); and (2) a sequence encoding a functional equivalent of S. pyogenes DNase B enzyme, the DNA being substantially free of DNA that does not encode the amino acid sequence of FIG. 4 (SEQ ID NO: 9) or a functional equivalent of S. pyogenes DNase B enzyme except for a leader peptide fused to the amino terminus of S. pyogenes DNase B enzyme operatively linked to at least one control sequence compatible with a suitable bacterial host cell in a manner allowing the transformed bacterial host cell to express the Streptococcus pyogenes DNase B encoded by the DNA incorporated within the expression vector in a detectable quantity; (b) using the cultured bacterial host cell to express the DNase B enzyme; and (c) purifying the enzyme from the cultured bacterial host cell to yield an enzyme substantially free of mitogenic activity.