Patent ID: 6780593
Filing Date: 2004-08-24
Classification: C12Q

Abstract:
A method for mapping a DNA molecule comprising the steps:(A) breaking the DNA molecule in order to obtain DNA fragments, wherein the size of the fragments depends on a selected resolution, (B) distributing said fragments in receptacles in order to have an amount of DNA between about 0.5 to 1.5 DNA haploid genome equivalent per receptacle, (C) amplifying the DNA contained in the receptacles by an amplification method comprising the following steps: i) a first amplification by means of a primer comprising 10 to 30 defined nucleotides at its 5â€² end and 5 to 10 random nucleotides at its 3â€² end, ii) a second amplification by means of a primer comprising at least the defined oligonucleotides of the 5â€² end of the primer used in step i), (D) detecting the presence or absence of markers in the receptacles (E) calculating an order of the markers and a distance between the markers to obtain a map of the DNA molecule.