Patent ID: 7943335
Filing Date: 2011-05-17
Classification: C12P

Abstract:
1. A process for the production of diacylglycerol, said process comprising the steps of: (i) reacting triacylglycerol with water and a lipase enzyme preparation at a temperature ranging from 20° C. to 90° C. to obtain a mixture of diacylglycerol, monoacylglycerol and free fatty acid; (ii) removing water content in the mixture of step (i) by way of dehydration; and (iii) separating the monoacylglycerol, free fatty acid and residual triacylglycerol by at least one separation method to obtain a high-purity diacylglycerol; wherein the amount of water in the reaction mixture of step (i) is from 20 to 180 parts by weight per 100 parts by weight of the enzyme preparation and step (i) is conducted until between 5% and 50% of free fatty acids is obtained, thus producing diacylglycerol of up to 96% purity at a high yield in a short period of time.