Patent ID: 6046000
Filing Date: 2000-04-04
Classification: C12N,C12Q

Abstract:
A method comprising:(a) obtaining a nucleic acid molecule comprising a chimeric gene, said chimeric gene comprising a first portion and a second portion, said first portion encoding KRE9 lacking a functional signal sequence and said second portion being a heterologous nucleic acid sequence;(b) transforming a yeast cell lacking a functional KRE9 gene with said nucleic acid molecule; and(c) determining whether said transformed yeast cell grows when supplied with a medium that permits growth of a yeast cell expressing KRE9 having a functional signal sequence, but does not permit growth of a yeast cell that does not express KRE9 having a functional signal sequence, wherein growth on said medium indicates that said heterologous nucleic acid sequence present in said yeast cell encodes a signal sequence, and wherein exnression of KR9 having-a functional signal sequence by said transformed Yeast cell does not cause cross-feeding of a neighboring yeast cell which does not exmress KRE9 having a functional signal sequence.