Patent ID: 8389213
Filing Date: 2013-03-05
Classification: C12N,C12Q

Abstract:
1. A method for the concentration of a gene, which is present in a small amount, from a DNA sample containing the gene present in a small amount and a gene, which is present in a large amount, wherein said DNA sample is prepared from a specimen where at least two kinds of microbes, organism tissues or cells are mixed, and obtained from nature, and said DNA sample is subjected to the following method to separate the gene present in a small amount from the gene present in a large amount, said method comprising: (a) dividing the DNA sample into two equal parts, one part being called a driver DNA fraction while the other part is called a target DNA fraction; (b) cleaving DNA in each of the driver DNA fraction and the target DNA fraction, such that the molecular weight of the driver DNA is made lower than the molecular weight of the target DNA, so that the average chain length of the driver DNA is 200˜300 base pairs and the average chain length of the target DNA is 1000 or more base pairs, further wherein the cleavage of DNA for the driver DNA fraction is performed with a four-base recognizing restriction enzyme and the cleavage of DNA for the target DNA fraction is performed with a 5-8 base recognizing restriction enzyme; (c) labeling the driver DNA; (d) mixing the target DNA with an excessive amount of the labeled driver DNA, then treating the DNA in the mixed solution to make the DNA single-stranded and then hybridizing the DNA; (e) by means of the labeling of the driver DNA, removing a double-stranded DNA formed by the driver DNA and the target DNA from the above mixed solution; and (f) carrying out the operations of (d) and (e) one or more times, where, instead of the target DNA, there is used a DNA solution obtained in (e) wherefrom the double-stranded DNA is removed.