Patent ID: 6592746
Filing Date: 2003-07-15
Classification: C12Q

Abstract:
A sensing probe for quantitative determination of hydrogen peroxide present in a body of fluid or fluid-containing material, which comprises:first and second oxygen sensors, each generating a signal proportional to oxygen content of a fluid electrolyte in contact with a respective sensor; first and second membranes disposed in series and separating said first oxygen sensor from said material, with said second membrane being disposed between said first membrane and said first oxygen sensor; a third membrane separating said second oxygen sensor from said material; said first membrane being permeable to hydrogen peroxide and oxygen, and having dispersed therethrough an immobilized enzyme which catalyzes the complete conversion of hydrogen peroxide to oxygen; said second and third membranes being hydrophobic and permeable to oxygen but not hydrogen peroxide; and a summer receiving oxygen-content-dependent signals from said first and second oxygen sensors and generating a resultant signal proportional to the difference between said oxygen-content-dependent signals, said difference being proportional to the concentration of hydrogen peroxide in said sample wherein the probe is an instant read probe.