Patent ID: 8211664
Filing Date: 2012-07-03
Classification: A61P,C12N,C12P

Abstract:
1. A method of making simvastatin comprising the steps of: (1) combining together monacolin J; an acyl thioester that donates a dimethylbutyryl moiety to the C8 hydroxyl group of monacolin J in the presence of functional LovD acyltransferase; and functional LovD acyltransferase comprising at least a 98% sequence identity to SEQ ID NO: 1 or SEQ ID NO: 2; and (2) allowing the functional LovD acyltransferase transfer the dimethylbutyryl group from the acyl thioester to regioselectively acylate the C8 hydroxyl group of monacolin J; so that simvastatin is made.