Patent ID: 7601831
Filing Date: 2009-10-13
Classification: C12Q

Abstract:
1. A diagnostic kit for detecting drug-resistant Mycobacterium tuberculosis cDNA comprising: (a) probes P1, P2 and P5 wherein each probe is linked to a separate magnetic bead; (b) bioactive primers with DNA labeling reagents wherein the DNA reagents are the compounds having the formula; wherein Fu represents a furocoumarin selected from the group consisting of angelicin and psoralen; BE represents none or a binding enhancer selected from the group consisting of C4-12 alkyl, alkyenyl, polyalkylamine and polyethylene glycol; and D represents a detectable group selected from the group consisting of: biotin, fluorescence, acridinium ester and acridinium-9-carboxamide, provided that D is biotin; (c) avidin enzyme complex or streptavidin enzyme complex; and (d) enzyme substrate, wherein the probe P1 is selected from the group consisting of: wherein the probe P2 is selected from the group consisting of: wherein the probe P5 is selected from the group consisting of: wherein positive detection of drug-resistant Mycobacterium tuberculosis cDNA is indicated when (i) the luminescence or color change associated with P1 is greater than the luminescence or color change associated with both P2 and P5, (ii) the luminescence or color change associated with P2 is less than the luminescence or color change associated with a P2 control, and (iii) the luminescence or color change associated with the P5 is less than the luminescence or color change associated with a P5 control, wherein control cDNA is drug-sensitive Mycobacterium tuberculosis cDNA.