Patent ID: 6037121
Filing Date: 2000-03-14
Classification: A61K,C07K

Abstract:
A method of identifying a DNA sequence which is the p33.sup.IG DNA sequence showvn in SEQ ID No. 1, comprisinga) subtractively hybridizing breast cancer cell line cflNAs with cDNA from normal mammary epithelial cells to obtain subtracted fractions;b) combining said subtracted fractions and using them to probe a cDNA libraryc) isolating cDNA clones obtained from step b) and randomly fragmenting them;d) transferring said DNA fragments to an expression vector to yield a genetic suppressor element library;e) introducing said genetic suppressor element library into normal cells;f) using the infected cells from step e) in a system which provides a means to identify an antisense oriented clone;g) selecting said antisense oriented clone; andh) isolating the cDNA of SEQ ID No. 1 from the clones selected in step g).