Patent ID: 7666654
Filing Date: 2010-02-23
Classification: C12N

Abstract:
1. A method for preparing viral material in a microcarrier cell culture, comprising (a) a first culturing phase which comprises an expansion of the cell culture volume by adding medium and microcarrier material, wherein a first maximum cell culture volume is obtained; (b) an infection step which is carried out after said first culturing phase and comprises the addition of infectious viral material to said microcarrier cell culture; (c) a second culturing phase which is carried out after said infection step and comprises a further expansion of the cell culture volume to a second maximum cell culture volume, wherein the viral material being generated during said second culturing phase; and (d) a harvesting step for obtaining the viral material from the microcarrier cell culture, wherein said second maximum culture volume is larger than said first maximum culture volume, wherein prior to the infection step (b) the cell culture volume is increased continuously, and wherein said second maximum culture volume is from two to seven times larger than said first maximum culture volume.