Patent ID: 9121004
Filing Date: 2015-09-01
Classification: C12M

Abstract:
1. A cell culture unit, comprising: a cell culture tub that defines a culture space for cultivating cells and has an air layer above the culture medium; a drainage channel that is connected to the cell culture tub to discharge used culture medium; an open culture medium reservoir that supplies new culture medium into the cell culture tub; and a droplet generator formed of a porous thin layer that is disposed between the cell culture tub and the open culture medium reservoir and supplies the culture medium from the open culture medium reservoir to the cell culture tub, using negative pressure of the air layer generated when the used culture medium is discharged from the cell culture tub so that an amount of the culture medium supplied from the open culture medium reservoir into the cell culture tub is substantially equal to an amount of the culture medium discharged from the cell culture tub, wherein the cell culture tub contains culture medium in the culture space and has wells separated from each other in which the cells proliferate in three-dimensional agglomerations, and the wells and the droplet generator are disposed separately.