Patent ID: 6153384
Filing Date: 2000-11-28
Classification: C12Q,G01N

Abstract:
A method of measuring the activity of a nucleic acid ligase in the presence of a potential nucleic acid ligase activity modulator, comprising:incubating a test ligation mixture comprising: a nucleic acid ligase; a tagged nucleic acid comprising template strand which comprises a tag; a labeling nucleic acid comprising a primary label, wherein the labeling nucleic acid is fully complementary to at least part of the template strand; a phosphate donor or a phosphate acceptor strand that is fully complementary to at least part of the template strand; and a potential activity modulator;binding the tag to a solid support, thereby binding the tagged nucleic acid to the solid support; and,determining whether the labeling nucleic acid is ligated to the phosphate donor or the phosphate acceptor strand and forms a duplex with the tagged nucleic acid by detecting the presence or absence of the labeling nucleic acid on the solid support, thereby determining the activity of the ligase in the test mixture.