Patent ID: 6146852
Filing Date: 2000-11-14
Classification: A61K,C07K,C12N

Abstract:
A process for producing recombinant parathyroid hormone (PTH) (1-84) protein comprising the steps of:(a) providing a yeast microorganism comprising:(1) a DNA sequence encoding the Saccharomyces mating factor .alpha.1 leader sequence as a leader sequence, said leader sequence lacking a STE13 recognition site; and(2) a DNA sequence encoding PTH (1-84) protein, wherein the DNA encoding the leader sequence and the DNA sequence encoding PTH (1-84) protein are operably linked;(b) culturing said yeast microorganism to allow expression of said PTH (1-84) protein, thereby producing PTH (1-84), wherein the yeast microorganism cleaves the PTH (1-84) protein from the leader sequence upon secretion of the PTH (1-84) protein from the yeast microorganism; and(c) purifying the resultant PTH (1-84) protein.