Patent ID: 6187305
Filing Date: 2001-02-13
Classification: A61K

Abstract:
A method of providing a therapeutic product in an effective amount to a mammal comprising the steps of:a) providing a DNA construct comprising:1) exogenous DNA encoding a product to be expressed in primary or secondary cells of mammalian origin;2) DNA sequences homologous with genomic DNA sequences in the primary or secondary cells of mammalian origin; and3) DNA sequences encoding at least one selectable marker;b) transfecting primary or secondary cells obtained from a mammal with the DNA construct provided in (a), thereby producing primary or secondary cells containing the DNA construct provided in (a);c) maintaining primary or secondary cells produced in (b) under conditions appropriate for homologous recombination to occur between DNA sequences homologous with genomic DNA sequences and genomic DNA sequences,thereby producing homologously recombinant primary or secondary cells of mammalian origin having the DNA construct of (a) integrated into genomic DNA of the primary or secondary cells; andd) introducing said homologously recombinant primary or secondary cells produced in (c) into a mammal in sufficient number to produce an effective amount of the therapeutic product in the mammal,wherein said recombinant primary or secondary cells are from the same species as said mammal.