Patent ID: 8465985
Filing Date: 2013-06-18
Classification: C09K,G01N,Y10T

Abstract:
1. A fluorescent probe comprising a compound represented by the following general formula (I): wherein Fluorophore A and Fluorophore B are fluorophores which emit fluorescence when they are irradiated with an excitation light of a wavelength of 600 to 950 nm, Fluorophore A has a property that it shows change of fluorescence characteristic before and after a specific reaction with a substance to be measured, and S represents a spacer which connects Fluorophore A and Fluorophore B, which compound shows substantial change in efficiency of fluorescence resonance energy transfer between Fluorophore A and Fluorophore B before and after the specific reaction with the substance to be measured, wherein Fluorophore A is represented by the following formula (AI): wherein R 1 and R 2 independently represent a C 1-18 alkyl group which may have a substituent; R 3 and R 4 independently represent hydrogen atom, or a substituent for detecting the substance to be measured, provided that R 3 and R 4 do not simultaneously represent hydrogen atom, and R 3 and R 4 may bind to each other to form a ring structure for detecting the substance to be measured; Y 1 and Y 2 independently represent —O—, —S—, —Se—, —CH═CH—, —C(R 5 )(R 6 )—, or —N(R 7 )—(wherein R 5 , R 6 and R 7 independently represent hydrogen atom, or a C 1-6 alkyl group which may have a substituent); n and n′ independently represent 0, 1 or 2; Z 1 and Z 2 independently represent a nonmetallic atom group required to form a benzo-condensed ring which may have a substituent or a naphtho-condensed ring which may have a substituent; L 1 , L 2 , L 3 , L 5 , L 6 and L 7 independently represent a substituted or unsubstituted methine group, L 4 represents a substituted methine group, provided that when n or n′ is 2, each two of L 1 and L 2 , or each two of L 6 and L 7 may be the same or different; M − represents a counter ion in a number required for neutralizing electrical charge, and the combination of R 3 and R 4 is a combination that provides change in the maximum excitation wavelength of Fluorophore A represented by the formula (AI) before and after detection of the substance to be measured; Fluorophore B is a tetramethylindodicarbocyanine derivative; and S is a spacer of a length so that FRET is induced between Fluorophore A and Fluorophore B.