Patent ID: 6326163
Filing Date: 2001-12-04
Classification: G01N,Y10S

Abstract:
A method for the medical-diagnostic analysis of the expression and incorporation and of the ratio of wild type to mutant in different tissue and organ regions by comparing a deviation, caused by a mutation, of the amino acid composition of a protein to be examined expressed in the region of the mutation with a corresponding protein which is expressed by a wild type lacking the mutation, whereina sample is taken from the organism at a site where the protein to be examined is expressed, is detected, and/or plays a physiological role;either the protein is concentrated or purified by methods of protein analysis, followed by a determination of its molecular weight, ora determination of the molecular weight of the protein to be examined is performed without a pretreatment of the sample;and the ratio of expression and incorporation of wild type to mutant is quantified.