Patent ID: 8080367
Filing Date: 2011-12-20
Classification: G01N

Abstract:
1. A method of identifying compounds which interfere with the binding of human IP6K2 protein to human HSP90 protein, comprising: contacting a cell with a test compound, wherein the cell comprises three recombinant DNA constructs, wherein said first polypeptide is a IP6K2 polypeptide and said second polypeptide is a HSP90 polypeptide, or said first polypeptide is a HSP90 polypeptide and said second polypeptide is a IP6K2 polypeptide; wherein said HSP90 polypeptide comprises amino acids 751-854 of SEQ ID NO: 2 or amino acids 630-731 of SEQ ID NO: 3 and said IP6K2 polypeptide comprises amino acids 131-140 of SEQ ID NO: 1; determining quantity of expression of the reporter gene in the presence of said compound, wherein a decrease in the expression of the reporter gene in the presence of the compound indicates that the compound interferes with the binding of human IP6K2 protein to human HSP90.