Patent ID: 6171804
Filing Date: 2001-01-09
Classification: G01N

Abstract:
A method for determining the relative orientation of two or more selected components of a macromolecule in solution with respect to the global molecular coordinate frame of the macromolecule comprising:(a) determining the orientation of the molecular frame for the selected components of the macromolecule in solution, thereby determining a set of rotational diffusion axes for the selected components; wherein the macromolecule comprises two or more components; and(b) determining the inter-component orientation in the macromolecule based on the orientation of the selected components with respect to the global molecular coordinate frame, and aligning the set of rotational diffusion axes of the component; wherein the relative orientation of the selected components of the macromolecule with respect to the global molecular coordinate frame of the macromolecule is determined.