Patent ID: 6929946
Filing Date: 2005-08-16
Classification: A61K,C07K,C12N

Abstract:
1. A method for reducing a tissue tropism of an adenovirus capsid of a subgroup C origin for liver cells as compared to the corresponding wild type adenovirus capsid, said method comprising: i) exchanging a first nucleic acid encoding a knob domain of a fiber protein of the adenovirus of subgroup C origin for a second nucleic acid encoding a knob domain of a fiber protein of an adenovirus selected from the group consisting of adenovirus 12, adenovirus 16, adenovirus 28, and adenovirus 40-L; ii) introducing the resulting nucleic acid from step i) into a cell capable of producing said adenovirus capsid; and iii) allowing said cell to produce said adenovirus capsid, thus reducing the tissue tropism of the adenovirus capsid for liver cells as compared to the corresponding wild type adenovirus capsid.