Patent ID: 6974792
Filing Date: 2005-12-13
Classification: A61K,A61P,C07K

Abstract:
1. A method of preparing a purified biologically active alpha 1-antitrypsin (α1-AT) preparation containing an α1-AT isomer having a pI of between 4.3 and 4.4 comprising: providing a starting material containing active and inactive α1-AT, wherein the starting material is a plasma fraction obtained from pooled human plasma; providing a hydroxyapatite substrate; passing said starting material containing α1-AT over said hydroxyapatite substrate; eluting a biologically active α1-AT biologically active α1-AT preparation having a pI of between 4.3 and 4.4; and passing said biologically active α1-AT preparation over an anion exchange material in the presence of a detergent.