Patent ID: 6335158
Filing Date: 2002-01-01
Classification: A61K,A61P,C07K,C12N,C12Q

Abstract:
A method of detecting the presence of nucleic acid of a human immunodeficiency virus in a sample, comprising the steps of:(a) providing a sample suspected of containing one or more nucleic acids encoding a protein of an immunodeficiency virus; (b) contacting the sample of step a with suitable PCR reagents that comprise at least a first and a second oligonucleotide primer that can anneal to the immunodeficiency virus nucleic acid, wherein the first primer sequence is complementary to a nucleic acid sequence from the region within amino acids 601 to 623 of the transmembrane protein of the MVP5180/91 strain of HIV-1 and the second primer is complementary to a known nucleotide sequence of a protein from HIV-1 or HIV-2; and (c) detecting the presence of a geometrically amplified product after incubation under conditions suitable for amplification using both primers.