Patent ID: 9018137
Filing Date: 2015-04-28
Classification: B01D,C07K,C12N,G01N

Abstract:
1. A method for the identification of a purifyable multi-specific polypeptide species (AiBj) which is a multimer consisting of at least two different specificity polypeptide chains (Ai, Bj), the method comprising the following steps: (A) generation of a library of nucleic acids for each of the different specificity polypeptide chains (A, B) forming the multimer, and narrowing the library size through antigen binding and/or activity assays; (B) individually expressing a sufficient amount of polypeptide chains (A1, A2, A3, . . . An; B1, B2, B3, . . . Bm) with each individual library member, and forming homomultimers therefrom; (C) using chromatography, for the determination of an analytically discriminative parameter, for each of the homomultimers; (D) selecting at least one pair (AiAi, BjBj) of homomultimers of different specificity polypeptide chains which are analytically sufficiently discriminated; (E) expressing the polypeptide chains from the library of step (A) corresponding to the homomultimers identified in step (D) in host cells such that a ternary mixture of homomultimers (AiAi; BjBj) and heteromultimers (AiBj) is produced by culture of the host cells; and (F) purifying the resulting ternary mixture by using chromatography.