Patent ID: 6150100
Filing Date: 2000-11-21
Classification: C12Q

Abstract:
A method for determining the sizes of at least 5 different microsatellite loci comprising the following steps:(a) isolating genomic DNA from a biological sample of a mammal to obtain genomic DNA;(b) amplifying the genomic DNA of at least 5 different microsatellite loci to obtain DNA amplification products, wherein at least 2 loci of said 5 different microsatellite loci are mononucleotide repeat loci and the remaining loci of said 5 microsatellite loci are(i) one or two dinucleotide repeat loci of class 2a,(ii) one or two dinucleotide repeat loci of class 2b, and(iii) zero or one pentanucleotide repeat locus, wherein said pentanucleotide repeat locus is TP53Alu,with the proviso that the DNA of APC and at least one dinucleotide repeat locus of class 2b are amplified; and thereafter(c) determining the sizes of the DNA amplification products of step (b), wherein the sizes of the DNA amplification products of step (b) are indicative of the sizes of said at least 5 different microsatellite loci.