Patent ID: 6552191
Filing Date: 2003-04-22
Classification: C07D

Abstract:
A method of extracting tetrodotoxin from the tissues of an organism comprising the steps of:a) obtaining a lixiviated solution-by soaking the tissues with water and a weak organic acid, then filtering; b) removing soluble proteins by heating the lixiviated solution at a temperature below the boiling point; c) obtaining an enriched tetrodotoxin solution by adjusting the pH of lixiviated solution with a water solution of a weak base, then putting the solution through a cation ion-exchange resin; d) removing inorganic salts and alkaline amino acids by adjusting the pH of the enriched tetrodotoxin solutions with an aqueous solution of a weak base, adsorbing the tetrodotoxin to a activated carbon and eluting the tetrodotoxin from the activated carbon; and e) crystallizing tetrodotoxin by concentrating the eluate and adjusting the pH of the eluate to pH from 8 to 10.