Patent ID: 7118900
Filing Date: 2006-10-10
Classification: C08J,C12Q,G01N,G03F,Y10T

Abstract:
1. A method of detecting a binding interaction between a biomolecule and a compound, comprising: (A)(i) providing a device having a first electrode in a first plane and a second electrode in a second plane; (ii) providing a polymerization composition between said first and second electrodes wherein different types of beads are suspended in said polymerization composition, said different types of beads having different biomolecules attached thereto; and wherein the different types of beads are distinguishable by a unique chemical or physical characteristic that identifies said bead type; (iii) generating an AC electric field with said first electrode such that the beads are moved to form one or more primary arrays of different types of beads; (iv) polymerizing said polymerization composition to form a first polymeric film, wherein said primary arrays of different types of beads are embedded in said first polymeric film; (v) removing said first polymeric film from said first electrode; (B)(i) providing a polymerization composition on said first electrode, wherein cells secreting said compound are suspended in said polymerization composition; (ii) generating an AC electric field with said first electrode such that the cells are moved to form one or more secondary arrays of cells; (iii) polymerizing said polymerization composition to form a second polymeric film, wherein said secondary arrays of cells are embedded in said second polymeric film; (iv) removing said second polymeric film from said first electrode; and (C) contacting said first polymeric film with said second polymeric film, thereby allowing said compound secreted by said cells to diffuse from said second polymeric film to said first polymeric film and to bind to a corresponding biomolecule to form a compound-biomolecule complex; and detecting the compound-biomolecule complex by identifying the unique chemical or physical characteristic of the bead to which the biomolecule that binds to the compound is attached.