Patent ID: 6410273
Filing Date: 2002-06-25
Classification: A61K,C12N

Abstract:
A method for the production of a methylated DNA selected from the group consisting of a plasmid DNA and a minicircle, wherein the method comprises transforming a prokaryotic cell with the DNA to be methylated and an expression cassette comprising a nucleic acid encoding a bacterial DNA methyltransferase, wherein the expression cassette is introduced into the prokaryotic cell before, after, or at the same time as the DNA to be methylated, culturing the transformed prokaryotic cell under conditions that permit expression of the bacterial DNA methyltransferase, whereby cytosine residues of 5â€²-CG-3â€² dinucleotides of the DNA to be methylated are methylated, and purifying the methylated DNA from the prokaryotic cell.