Patent ID: 6093370
Filing Date: 2000-07-25
Classification: B01J,B01L,C12Q,C40B

Abstract:
A polynucleotide separation method comprising the steps of:immobilizing each of single stranded-oligonucleotide probes each having a specific base sequence to each of a plurality of areas, said areas being independent and formed on the surface of a substrate,supplying a sample solution (containing polynucleotides onto said substrate,heating said sample solution up to a predetermined temperature and thereafter cooling he heated solution to thereby hybridize each of complementary polynucleotides separately to each of probes,replacing said sample solution above the substrate with a solution containing no polynucleotide, andheating the surface of the substrate at one area of said plurality of independent areas on the substrate up to a predetermined temperature, and thereby denaturing only a polynucleotide being hybridized complementarily to said probe immobilized on said area to extract said denatured polynucleotide.