Patent ID: 6602664
Filing Date: 2003-08-05
Classification: A61K,A61P

Abstract:
A method of assessing the sensitivity of an HIV-1 sample to CS-87 (3â€²-Azido-2â€², 3â€²-dideoxyuridine), comprising:a) isolating nucleic acid from the sample, b) hybridizing an oligonucleotide to the nucleic acid, wherein the oligonucleotide is complementary to: i) a portion of the reverse transcriptase region terminating at codon 70 at the 3â€² end, of the wild-type DNA sequence or its corresponding RNA; or ii) a portion of the reverse transcriptase region terminating at codon 70 at the 3â€² end, of the wild-type DNA sequence mutated at codon 70 or its corresponding RNA; c) subjecting the oligonucleotide to polymerization conditions; and d) ascertaining whether or not an oligonucleotide primer extended product is present.