Patent ID: 6221598
Filing Date: 2001-04-24
Classification: C12Q

Abstract:
A method of simultaneously determining the alleles present in at least three short tandem repeat loci from one or more DNA samples, comprising:a) obtaining at least one DNA sample to be analyzed;b) selecting a set of at least three short tandem repeat loci of the DNA sample to be analyzed which can be co-amplified, wherein the at least three short tandem repeat loci in the set comprises at least three loci selected from the group consisting of:HUMPOX, HUMTH01 and HUMCD4;HUMTPOX, HUMTH01 and HUMVWFA31;HUMHPRHUMFESFPS and HUMVWFA31;HSAC04 (ACTBP2), HUMCYP19 and HUMPLA2A1;HUMAPOA2, HUMCYP19 and HUMPLA2A1;HUMCD4, HUMCSF1PO and HUMTH01;HUMCYP19, HUMFABP and HUMPLA2A1;HUMCYP19, HUMHPRTB and HUMPLA2A1;HUMHPRHUMFESFPS and HUMLIPOL;HUMF13AO1, HUMFABP and HUMCD4;HUMHPRHUMBFXIII (F13B) and HUMPLA2A1;HUMHPRHUMBFXIII (F13B) and HUMTPOX;HUMHPRHUMBFXIII (F13B) and HUMFESFPS;HUMCSF1PO, HUMTPOX and HUMCD4;HUMHPRHUMFESFPS and HUMMYOPK (Myotonic);HUMCSF1PO, HUMTH01 and HUMCD4;HUMCSF1PO, HUMTH01 and HUMVWFA31; andHUMHPRHUMBFXIII (F13B) and HUMLIPOL;c) co-amplifying the set of at least three short tandem repeat loci in a multiplex amplification reaction, thereby producing a mixture of amplified alleles from each of the co-amplified loci in the set; andd) evaluating the amplified alleles in the mixture to determine the alleles present at each of the co-amplified loci in the set.