Patent ID: 9028771
Filing Date: 2015-05-12
Classification: B01D,B01L,G01N,Y10S

Abstract:
1. A method of performing a saturation assay for detecting an analyte in a sample, comprising: a. providing a labeled analyte analogue and an unlabeled analyte binding reagent; b. concentrating a bound fraction of labeled analyte analogue resulting from a competitive binding event between the analyte and the labeled analyte analogue for the unlabeled analyte binding reagent at a primary trapping zone; c. concentrating a free or unbound fraction of labeled analyte analogue which is not bound to the unlabeled analyte binding reagent at a secondary trapping zone; and d. detecting the free or unbound fraction of the labeled analyte analogue concentrated at the secondary trapping zone, wherein the primary trapping zone comprises a capillary membrane of a pore size which concentrates the bound fraction of labeled analyte analogue, and the secondary trapping zone comprises a capillary membrane of a pore size which concentrates the free or unbound fraction of labeled analyte analogue, and wherein the amount of the free or unbound fraction of the labeled analyte analogue concentrated at the secondary trapping zone is indicative of the amount of analyte in the sample.