Patent ID: 8715933
Filing Date: 2014-05-06
Classification: C12Q

Abstract:
1. A method for preparing and analyzing a target analyte, the method comprising: a. providing a double-stranded DNA template having a first and a second DNA strand, each DNA strand having a 5′ end and a 3′ end, b. contacting the double-stranded DNA template with a nicking endonuclease to form a nick at a sequence-specific nicking location on the first DNA strand, c. conducting a base extension reaction on the first DNA strand along a corresponding region of the second DNA strand, said reaction starting at the nick and progressing toward the 3′ end of the first DNA strand to thereby form a single-stranded flap on the double-stranded DNA template adjacent to the sequence-specific nicking location, to thereby prepare the target analyte, and d. monitoring changes in an electrical property across a nanopore as the target analyte is translocated therethrough, the changes in the electrical property being indicative of double-stranded regions of the target analyte and of the single-stranded flap regions.