Patent ID: 6258536
Filing Date: 2001-07-10
Classification: C12Q

Abstract:
A method for detecting a BRCA1 gene functional mutation in target cells comprising the steps of:detecting expression of a plurality of down-stream genes of BRCA1 in a sample of (a) target cells and (b) reference cells having a wild-type BRCA1 gene, the reference cells being otherwise substantially similar to the target cells, the down-stream genes being up- or down-regulated by the wild-type BRCA1 gene, wherein said down-stream genes comprise at least one BRCA1 up-regulated gene selected from the group consisting of gadd45, histone H4, EGR1, ATF3, Jun-B, Rev-Erb .alpha., Etr101, A20, tristetraproline (TTP), Gem GTPase, Br140, R12810, PM-sc175, ARD1, hepatic leukemia factor, amphiregulin, TR3, orphan receptor (NAK1), fibroblast activating protein-.alpha., IL4 receptor .alpha., R41997, H81220, KIAAA00027, and Nucleotide binding protein, or at least one BRCA1 down-regulated gene selected from the group consisting of Pim-1, Pigf, GF14.OMEGA., Mki67a and prothymosin-.alpha.; andcomparing the expression of the down-stream genes in the target cells and the reference cells, wherein a difference in the expression between the target cells and reference cells suggests a BRCA1 functional mutation in the target cells.