Patent ID: 8039256
Filing Date: 2011-10-18
Classification: C12N

Abstract:
1. A method of culturing mesenchymal stem cells comprising: adjusting the specific gravity of aqueous cell culture medium to between 1.06 and 1.10 at 37° C. by addition of at least one substance selected from the group consisting of silica fine powder coated by polyvinyl pyrrolidone, a water soluble copolymer of sucrose and epichlorohydrin, and a water soluble compound comprising a triiodo aromatic ring; seeding at least one selected from the group consisting of bone marrow solution, umbilical cord blood, peripheral blood, synovial membrane, and amniotic membrane in a vessel using a sufficient volume of said specific gravity adjusted aqueous cell culture medium such that said vessel is filled; and culturing the cells on the ceilin side surface of the vessel at a temperature of 37±2° C.