Patent ID: 8470328
Filing Date: 2013-06-25
Classification: C07K

Abstract:
1. A method of producing a drug formulation, comprising: a) placing a starting material containing an antibody on a protein A affinity chromotography column, b) desorbing said antibody from said column using a buffer solution adjusted to pH 4.0 to 5.0 comprising arginine and/or an arginine derivative, c) recovering the antibody, and d) providing a drug formulation comprising the antibody recovered, wherein said arginine derivative is selected from the group consisting of acetyl arginine, agmatine, L-arginic acid, N-a-butyroyl-L-arginine, and N-α-pyvaloyl arginine, wherein the concentration of said arginine and/or derivative thereof provides an antibody recovery rate equivalent to that of pH 3.5 sodium citrate buffer solution.