Patent ID: 7141240
Filing Date: 2006-11-28
Classification: A61K,C07K

Abstract:
1. A method for constructing a glucose dependent insulin-secreting cell, in vitro, the method comprising the steps of: providing an insulin-secreting cell, in vitro; isolating from a proglucagon gene a minigene construct consisting of (a) the coding region for a protein selected from the group consisting of glucagon-like peptide-1 (GLP-1) and an analog of GLP-1, said analog of GLP-1 being further selected from the group consisting of SEQ ID NO. 1 and SEQ ID NO. 3; (b) a partial fragment of an IP sequence at the 5′-end; and (c) a nucleotide sequence at the 3′-end wherein the nucleotide sequence at the 3′-end is selected from the group consisting of, an entire IP-2 sequence and an entire IP-2 sequence along with a partial fragment of a glucagon-like peptide-2 (GLP-2) sequence; operably linking a promoter to the minigene construct; inserting or ligating the minigene construct into a plasmid; and transfecting the plasmid into the insulin-secreting cell, whereby said insulin-secreting cell produces GLP-1 or an analog of GLP-1 in a glucose dependent manner.