Patent ID: 7871795
Filing Date: 2011-01-18
Classification: C12N

Abstract:
1. An isolated cell comprising a nucleic acid molecule that encodes a protein or polypeptide of interest wherein said nucleic acid molecule comprises: a) one or more target binding domains that target binding of the nucleic acid molecule that encodes the protein or polypeptide of interest to an abundantly expressed target pre-mRNA within the cell, wherein said nucleic acid molecule is recognized by nuclear splicing components within the cell and wherein the abundantly expressed target pre-mRNA is selected from the group consisting of pre-mRNAs encoding albumin, casein, myosin and fibroin; b) a splice region; c) a spacer region that separates the splice region from the target binding domain; and d) a nucleotide sequence encoding the a protein or polypeptide of interest to be trans-spliced to the target pre-mRNA; wherein said nucleic acid molecule encodes a polypeptide of interest heterologous to the target pre-mRNA.