Patent ID: 7298461
Filing Date: 2007-11-20
Classification: G01N,G02B

Abstract:
1. Far field light microscopical method for analysing at least one object having a subwavelength size in at least one spatial direction to obtain spatial information of the object, in particular size and topology thereof, comprising the steps of: labelling the at least one object with one or more suitable optical markers; providing suitably structured illumination light to at least partially illuminate the at least one object; subjecting the at least one object to the structured illumination light; detecting an optical response of the at least one object; obtaining the spatial information of the at least one object by comparing the obtained response with simulation data of an optical response of at least one object having known spatial information, wherein the spatial information comprises at least one of the following information: size of the object in at least one spatial direction; topology of the object in at least one spatial direction; at least one distance between at least two obiects in at least one spatial direction.