Patent ID: 7135284
Filing Date: 2006-11-14
Classification: C12Q

Abstract:
1. A method of labeling an oligonucleotide, comprising the steps of: (a) hybridizing a first oligonucleotide to a second oligonucleotide, wherein the first oligonucleotide consists of, from 3′ to 5′: a Substrate Hybridization Domain adjoining a Signal Template Domain, wherein: and the second oligonucleotide comprises; from 3′ to 5′: a Template Hybridization Domain adjoining a Target Binding Domain, wherein: (b) extending the second oligonucleotide with a DNA polymerase in the presence of labeled nucleotides to create an oligonucleotide Probe having from 5′ to 3′ an unlabeled Target Binding Domain adjoining a Template Hybridization Domain adjoining a labeled Signal Domain.