Patent ID: 6265214
Filing Date: 2001-07-24
Classification: A61K,C07K,Y02A,Y10S

Abstract:
A method of generating a continuous cell line which produces a factor capable of inducing human monocytes to a cytotoxic state, the method comprising the steps of:(a) immortalizing human T-cells to produce continuous cell clones;(b) identifying a clone which produces a factor having the following characteristics:i. capability of inducing human monocytes to a cytotoxic state;ii. retention of biological activity following treatment at pH 2 for one hour;iii. retention of biological activity following treatment at 60.degree. C. for one hour;iv. ability to bind to Matrex Gel Red A under low-salt conditions and elute from Matrex Gel Red A under high-salt conditions; andv. retention of biological activity in the presence of anti-serum to interferon gamma, interferon alpha, or a combination of anti-sera to interferon alpha and gamma; and(c) culturing the clone to produce the continuous cell line.