Patent ID: 8404456
Filing Date: 2013-03-26
Classification: A61P,C07K,G01N,Y10T

Abstract:
1. A method for determining the presence of anti-blood group antigen reactive antibodies in serum of a subject, said method comprising: a) providing a collection of blood group antigens coated onto a solid surface, wherein the collection of blood group antigens comprises one or more blood group antigens selected from the group consisting of: b) adding said serum from said subject to said blood group antigen coated solid surface; c) incubating said serum and said blood group antigen coated solid surface for sufficient time for anti-blood group antigen reactive antibodies in said serum to bind to said blood group antigens; d) removing components in said serum which do not specifically bind to said blood group antigen coated solid surface; e) incubating said solid surface with ligands capable of specifically binding to said anti-blood group antigen reactive antibodies bound to said blood group antigens; wherein said ligands are labeled with different labels such that one blood group antigen differs from at least one other blood group antigen by being labeled with said differently labeled ligands; f) removing said labeled ligands that are not bound to said anti-blood group antigen reactive antibodies; and g) detecting the presence of said differently labeled ligands bound to said anti-blood group antigen reactive antibodies to determine the presence or absence of said reactive antibodies.