Patent ID: 7985536
Filing Date: 2011-07-26
Classification: A01N

Abstract:
1. A method for regulating nitric oxide production in an organ or tissue comprising: a) perfusing an isolated organ or tissue at near physiologic temperature with a warm perfusion solution comprising: (i) a bicarbonate-buffered basal medium; (ii) chemical energy substrates including at least one component of the citric acid cycle; (iii) nucleic acids; (iv) purines and pyrimidines (v) colloid; (vi) at least one growth factor; (vii) lipid; (viii) at least one hormone; (ix) an oxygen carrier; and (x) arginine; b) monitoring the concentration of NO or a stable end-product thereof in said perfusion solution during perfusion; and c) (i) increasing the amount of arginine in said perfusion solution when the concentration of NO or stable end-product thereof decreases;