Patent ID: 7615368
Filing Date: 2009-11-10
Classification: B01J,B01L,C40B,G01N

Abstract:
1. A microarray of discrete polypeptides on a slide, wherein each polypeptide is of at least 50 amino acids in length, wherein said microarray comprises 1000 or more discrete regions of distinct polypeptide per cm 2 of slide wherein discrete regions have a diameter of from 20 to 200 μm, produced by the method of: (a) loading an aqueous solution of a selected polypeptide of at least 50 amino acids in length in a reagent-dispensing device having an elongate capillary channel adapted to hold a quantity of the reagent solution and having a tip region at which the solution in the channel forms a meniscus, (b) tapping the tip of the dispensing device against a surface of a planar solid support at a defined position, with an impulse effective to break the meniscus in the capillary channel and deposit a selected volume between 0.002 and 2 nl of solution on the surface of the planar solid support, wherein said planar solid support comprises a cationic film which binds said polypeptide, and (c) repeating steps (a) and (b) until said microarray is formed.