Patent ID: 7419835
Filing Date: 2008-09-02
Classification: B01J,C40B,G01N,Y10T

Abstract:
1. A method for screening of compounds for binding differentiation at various drug target binding sites, for use with a device measuring the enthalpy of reaction for such binding, comprising: merging not less than one test ligand with not less than one target compound at not less than one first location on the device measuring enthalpy of reaction, wherein said target compound includes not less than one binding site of interest and not less than one binding site to be avoided; merging not less than one test ligand with not less than one target compound in the presence of at least one first blocking agent at not less than one second location on the device measuring enthalpy of reaction, wherein said target compound includes not less than one binding site of interest and not less than one binding site to be avoided; detecting a first heat of reaction for said merged not less than one test ligand and said not less than one target compound at said first location; detecting a second heat of reaction for said merged not less than one test ligand with said not less than one target compound in the presence of a blocking agent at said second location; comparing said first and second heats of reaction; and determining a binding operation has occurred at the binding site of interest of the first location when the comparing step finds the first heat of reaction is greater than the second heat of reaction.