Patent ID: 7736886
Filing Date: 2010-06-15
Classification: C12N

Abstract:
1. A recombination system comprising: a transgenic recombination construct capable of being inserted into the chromosomal DNA of a eukaryotic organism said construct comprising in a 5′- to 3′-orientation; a first homology sequence A; at least one recognition sequence for site-directed induction of DNA double-strand breaks; and a second homology sequence B, where all recognition sequences for site-directed induction of DNA double-strand breaks are located between homology sequences A and B; wherein the homology sequences A and B have at least 20 base pairs and at least 70% homology that allows for homologous recombination to each other; and an enzyme suitable for inducing DNA double-strand breaks at a recognition sequence for the site-directed induction of DNA double-strand breaks or a nucleic acid sequence encoding said enzyme; wherein after homologous recombination of homology sequences A and B the resulting transgenic sequence derived from said transgenic recombination construct does not comprise any recognition site for said enzyme suitable for inducing DNA double-strand breaks.