Patent ID: 6210913
Filing Date: 2001-04-03
Classification: A61P,C07K

Abstract:
An assay method for identifying agents that block interactions between the cytoplasmic domain of a phosphorylated beta subunit of an integrin and a cytoplasmic signaling partner comprising the steps of:a) incubating a peptide comprising the phosphorylated cytoplasmic domain of the beta subunit of an integrin with a cytoplasmic signaling partner in the presence and absence of an agent, wherein said signaling partner specifically binds the phosphorylated cytoplasmic domain of said beta subunit intracellularly,b) detecting a change in the level of interaction between said phosphorylated cytoplasmic domain of said beta subunit and said cytoplasmic signaling partner; andc) determining whether said agent blocks the binding of said cytoplasmic signaling partner to said peptide in step a), wherein a decrease in the level of interaction in step b) is indicative of blocking interaction.