Patent ID: 7910304
Filing Date: 2011-03-22
Classification: B01J,B01L,B82Y,C12Q,G01N

Abstract:
1. A method for sequencing target nucleic acids, said method comprising: (a) providing a random array of said target nucleic acids; (b) hybridizing a first and second population of oligonucleotide probes to said array; (c) ligating oligonucleotide probes from said first and second populations that are hybridized to adjacent positions on said target nucleic acids to produce ligated probes; (d) collecting a signal produced by said ligated probes; (e) analyzing said signal to identify at least one nucleotide of said target nucleic acids; (f) repeating steps (b)-(e) to provide multiple cycles of sequence information for said target nucleic acids, such that at least one nucleotide of said target nucleic acids is identified in more than one cycle; (g) assembling said multiple cycles of sequence information to provide sequences of all or a portion of said target nucleic acids.