Patent ID: 7588921
Filing Date: 2009-09-15
Classification: C12Q

Abstract:
1. A method for identifying the presence of one or more body fluids from a human being in a single or mixed stain the fluids selected from the group consisting of non-menstrual blood, menstrual blood, saliva and semen, comprising the steps of: a) obtaining a sample stain comprising a body fluid from a human being; b) extracting total ribonucleic acid (RNA) from the sample stain; c) treating the total RNA from step b) with an enzyme d) initiating a reverse-transcriptase (RT) reaction by treating the total RNA from step c) with random decamers to produce cDNA; e) amplifying a pair of body-fluid specific genes (BFGs) selected from the pairs: ALAS2 and SPTB, specific for non-menstrual blood, HTN3 and STATH, specific for saliva, PRM1 and PRM2, specific for semen, and MMP-7 and MMP-10, specific for menstrual blood, wherein said amplifying utilizes two primers and a probe for each BFG; f) amplifying a housekeeping gene (HSK) selected from one of GAPDH and GNAS, wherein said amplifying utilizes two primers and a probe for each HSK; g) determining the presence of the body fluid in the stain by measuring the cycle threshold (Ct) value-of the house keeping gene (HSK) and the Ct values of both BFGs, and then subtracting the Ct value of each BFG from the Ct value of the HSK gene, wherein a positive dCt (HSK-Ct minus BFG-Ct) value of each BFG indicates the presence of the corresponding body fluid in the stain.