Patent ID: 8865241
Filing Date: 2014-10-21
Classification: A23L,C08B,C12N,C12P

Abstract:
1. A process of preparing a clarified diutan product of native diutan produced by a Sphingomonas mutant strain genetically modified from a wild-type Sphingomonas strain ATCC 53159, wherein a phaC gene in said Sphingomonas mutant strain has been selectively mutated such that the mutant strain produces native diutan without producing polyhydroxybutyrate (PHB) due to lack of expression of phaC protein in said mutant strain, comprising the steps of: a) heating an aqueous diutan solution containing native diutan of the mutant strain to a clarification temperature of 30-70° C., b) treating the heated aqueous diutan solution from step a) with at least one chelating agent, c) treating the diutan solution from step b) with a lysozyme enzyme, a protease, or both, and d) recovering the clarified diutan product by precipitation with an alcohol.