Patent ID: 9163263
Filing Date: 2015-10-20
Classification: C12N,C12P,C12Y

Abstract:
1. A recombinant host cell comprising a nucleic acid encoding a polypeptide having isoprene synthase activity, wherein the polypeptide comprises one or more amino acid substitution(s) at one or more residue position(s) corresponding to positions in the amino acid sequence of SEQ ID NO:1, wherein said one or more amino acid substitution(s) is selected from the group consisting of: X118E, X36W, X22K, X228Y, X448L, X488F, X467H, X331P, X448I, X392Y, X448V, X282H, X383Y, X511 Y, X448E, X376M, X488L, X461A, X414I, X282W, X493E, X392S, X448Q, X282Y, X537N, X447Y, X240C, X538R, and X510C, and wherein the polypeptide has increased k cat compared to a parent polypeptide which does not comprise said one or more amino acid substitution(s), and wherein said polypeptide has at least 80% sequence identity with SEQ ID NO:1.