Patent ID: 8324372
Filing Date: 2012-12-04
Classification: C07C,C07H,C08G,C12N,C12Q

Abstract:
1. A method for isolating polynucleotides in a cell-containing sample from a polymerase chain reaction inhibitor selected from the group consisting of hemoglobin, peptides, faecal compounds, humic acids, mucousal compounds, DNA binding proteins, and saccharides, or any combination thereof, the method comprising: contacting the sample with a lysis solution containing a lysis reagent, said lysis reagent comprising a detergent, and a plurality of binding particles, wherein the binding particles comprise PAMAM(Generation 0) covalently bound to the surface of the binding particles, said lysis solution having a pH between 4 and 8; heating the sample contacted with the lysis reagent, thereby lysing cells contained in the sample in the lysis solution and concomitantly binding the polynucleotides to the PAMAM of the binding particles to create binding particles bound with polynucleotides and a remaining residual solution; compacting the binding particles bound with polynucleotides by bringing the binding particles bound with polynucleotides in contact with one another; removing a substantial portion of the residual solution; and contacting the compacted binding particles with a release solution having a pH of ≧9, thereby releasing the polynucleotides from the PAMAM of the binding particles.