Patent ID: 6060278
Filing Date: 2000-05-09
Classification: A61K,C07K

Abstract:
A method for the in-vitro preparation of a recombinant troponin C-troponin T-troponin I complex comprising the steps of:a. expressing a recombinant human troponin C, a recombinant human troponin T, and a modified recombinant troponin I; andb. mixing the recombinant human troponin C, the recombinant human troponin T, and the modified recombinant troponin I in an aqueous medium at a pH of about 7.5 in the presence of at least one alkaline earth salt,wherein said modified recombinant troponin I consists of the native human cardiac troponin I protein sequence with an N-terminal extension, said extension consisting of about 5 to about 8 amino acid residues including an N-terminal methionine, and wherein the expression in E. coli of said modified troponin I is increased compared to that of a recombinant human cardiac troponin I having a native protein sequence.