Patent ID: 8771951
Filing Date: 2014-07-08
Classification: C12Q

Abstract:
1. A method for amplifying a DNA of interest, comprising: obtaining a raw sample comprising DNA; performing a first PCR on the raw sample to produce a first amplicon; diluting the first amplicon; and performing a second PCR thereon until all primers used in the second PCR reaction are consumed to produce a second amplicon, thereby amplifying the input sample DNA to a final amplified DNA product concentration that is limited by the primer concentration in the second PCR reaction, said second PCR reaction independent of the amount or purity of the DNA comprising the original sample, wherein primers for the second PCR reaction target DNA sequences that are contained within the amplified product of the first PCR reaction and wherein primers for the second PCR reaction are a set of multiple exon-specific primers having sequences shown in at least SEQ ID NO:15 and 16 and additional primer pairs selected from the group consisting of SEQ ID NOs:17-27.