Patent ID: 6251610
Filing Date: 2001-06-26
Classification: C12N,C12Q

Abstract:
A method effectively to join both strands of a double stranded DNA into a single strand of DNA, wherein said double-stranded DNA consists of a first strand and a second strand wherein said single strand of DNA comprises at least a portion of said first strand and said second strand, wherein said method comprises:a) amplifying said double-stranded DNA to form amplified DNA using a pair of primers wherein one of said primers comprises a reverse complementary sequence of nucleotides;b) denaturing said amplified DNA to form single-stranded DNA;c) allowing intrastrand hybridization of said single-stranded DNA to occur; andd) elongating said intrastrand hybridized single-stranded DNA to yield panhandle DNA which upon denaturation yields a single-stranded DNA comprising sequence of said both strands of said double stranded DNA.