Patent ID: 8147842
Filing Date: 2012-04-03
Classification: C12N

Abstract:
1. A mutant luciferase selected from the following (a) to (d): (a) a protein comprising the amino acid sequence of SEQ ID NO: 2, but in which the methionine at position 178 is substituted with another amino acid; (b) a protein comprising an amino acid sequence at least 90% identical to SEQ ID NO: 2, wherein the residue corresponding to the methionine at position 178 in SEQ ID NO: 2 is substituted with another amino acid, and wherein said protein has luciferase activity with an emission spectral peak of 449 nm or less; (c) a protein comprising residues 19-553 of SEQ ID NO: 2, but in which the methionine at position 178 is substituted with another amino acid; and (d) a protein comprising an amino acid sequence at least 90% identical to residues 19-553 of SEQ ID NO: 2, wherein the residue corresponding to the methionine at position 178 in SEQ ID NO: 2 is substituted with another amino acid, and wherein said protein has luciferase activity with an emission spectral peak of 449 nm or less.