Patent ID: 6133229
Filing Date: 2000-10-17
Classification: C07K,C12N

Abstract:
A method of stabilizing an aqueous enzyme comprising the step of contacting the enzyme selected from the group consisting of peroxidase enzymes, alkaline phosphatase enzymes and antibody conjugates thereof with an aqueous solution of a protein stabilizer additive comprising:a. a tris(hydroxymethyl)methyl compound of formula 1;(HOCH.sub.2).sub.3 C--R (1)wherein R is: NH.sub.2 ; NR.sup.1 R.sup.2 wherein R.sup.1 and R.sup.2 may be independently: H, C.sub.1 -C.sub.4 alkyl, C.sub.1 -C.sub.4 hydroxyalkyl, C.sub.1 -C.sub.4 alkyl sulfonate, C.sub.1 -C.sub.4 hydroxyalkyl sulphonate; C.sub.1 -C.sub.4 alkyl NHC(CH.sub.2 OH).sub.3 ; C.sub.1 -C.sub.4 alkyl carboxylate;b. a cationic polyelectrolyte selected from the group consisting of copolymers of vinyl pyrollidone and quaternary methyl methacrylate and DEAE dextran;c. a polyol selected from the group consisting of glycerol, ethylene glycol, sorbitol and lactitol; andd. one or more salts selected from the group consisting of salt of calcium, magnesium and cobalt.