Patent ID: 6365158
Filing Date: 2002-04-02
Classification: A61K,A61P,C07K,Y02A,Y10S

Abstract:
A method of generating a neutralizing antitoxin directed against Clostridium difficile toxin B comprising:a) providing: i) a purified recombinant fusion protein comprising a non-toxin protein sequence and a portion of the Clostridium difficile toxin B sequence of SEQ ID NO:10, wherein said portion contains at least amino acids 1750-2360 of said Clostridium difficile toxin B sequence, and wherein said recombinant fusion protein is expressed in soluble form, at a level greater than one milligram per liter of host cell culture, when expressed in a host cell, and ii) an avian host; and b) immunizing said avian host with said recombinant fusion protein so as to generate an antitoxin capable of neutralizing Clostridium difficile toxin B in vivo.