Patent ID: 6324479
Filing Date: 2001-11-27
Classification: A61K,C07K

Abstract:
A method of determining a level of activity of a protein in a cell type, comprising determining a level of perturbation to said protein at which similarity is greatest between a diagnostic profile and a perturbation response profile extracted from perturbation response curves for said determined level of perturbation to said protein, said diagnostic profile having been obtained by a method comprising measuring a first plurality of cellular constituents in a cell of said cell type, said perturbation response curves being the products of a method comprising:(i) providing perturbation response profiles of said protein for said cell type, wherein said perturbation response profiles are obtained by measuring a second plurality of cellular constituents in a cell of said cell type at a plurality of discrete levels of perturbation to said protein, and(ii) interpolating said perturbation response profiles so that a perturbation response profile may be extracted over a range of levels of perturbation to said protein, wherein said interpolated response profiles comprise said perturbation response curves,wherein said determined level of perturbation to said protein represents said protein activity level in said cell type.