Patent ID: 6849400
Filing Date: 2005-02-01
Classification: C12Q

Abstract:
1. A method for detecting a fusion nucleic acid comprising the steps of: a) providing a sample containing a first single-stranded fusion nucleic acid comprising a bcr-abl splice junction site; b) contacting under nucleic acid amplification conditions: c) amplifying the fusion nucleic acid in a single isothermal nucleic acid amplification reaction using the first primer to produce a plurality of second nucleic acid strands complementary to at least a portion of the first single-stranded fusion nucleic acid that contains the bcr-abl splice junction site, wherein each second nucleic acid strand comprises: d) hybridizing the second nucleic acid strands with an oligonucleotide probe under hybridization conditions in which the probe hybridizes to the first or second probe binding site, thereby forming a probe; target hybrid; and e) detecting the probe:target hybrid as an indication of the presence of the fusion nucleic add in the sample.