Patent ID: 7344850
Filing Date: 2008-03-18
Classification: C12Q,G01N

Abstract:
1. A method of identifying a compound that inhibits the ability of a glycosyltransferase to bind a substrate comprising: combining a glycosyltransferase, a labeled substrate, and a compound, in a reaction vessel, under conditions known to be suitable for the glycosyltransferase to bind the labeled substrate, measuring the amount of labeled substrate bound to the glycosyltransferase, and comparing the amount to a standardized amount to identify a relative increase or decrease in substrate bound glycosyltransferase, thereby identifying a compound that modulates the ability of the glycosyltransferase to bind the substrate, wherein the glycosyltransferase is a GT-A or GT-B, NDP-glycosyltransferase, the label is fluorescein and, the labeled substrate is the UDP-GlcNAc (hexose donor) analogue: