Patent ID: 7390627
Filing Date: 2008-06-24
Classification: C07K,G01N,Y10T

Abstract:
1. An immunological assay for the detection and/or quantification of human MDA-modified LDL (malondialdehyde-modified low density lipoprotein) and human OxLDL (oxidized low density lipoprotein) in a sample derived from the body fluids or tissues of a human being, said assay comprising: (a) contacting the sample with a first antibody that has high affinity for human MDA-modified LDL and human OxLDL wherein the first antibody binds to the same epitope as monoclonal antibody mAb-4E6 produced by hybridoma Hyb4E6 deposited at the BCCM (Belgian Coordinated Collections of Microorganisms) under deposit accession number LMBP 1660 CB on Apr. 24, 1997; and (b) thereafter visualizing and/or quantifying a binding reaction between the first antibody and the MDA-modified LDL and OxLDL present in the sample; wherein the MDA-modified LDL and OxLDL for which the first antibody has high affinity contain at least 60 substituted lysine moieties per apo B-100 (apolipoprotein B-100) moiety.