Patent ID: 6372813
Filing Date: 2002-04-16
Classification: G01N

Abstract:
A method for preparing a crosslinked polyacrylamide hydrogel or hydrogel array comprising one or more attached biomolecule(s), said method comprising:(a) obtaining a polyacrylamide reactive prepolymer that includes one or more reactive site(s) capable of 2+2 photocycloaddition; (b) placing said polyacrylamide reactive prepolymer on a solid support; (c) crosslinking said polyacrylamide reactive prepolymer to obtain a crosslinked polyacrylamide hydrogel or hydrogel array; (d) obtaining one or more biomolecule(s) that includes one or more reactive site(s) capable of 2+2 photocycloaddition; and (e) contacting the product of step (c) with the product of step (d) and irradiating with ultraviolet light sufficient for 2+2 photocycloaddition to occur such that said crosslinked polyacrylamide hydrogel or hydrogel array comprising one or more biomolecule(s) attached by 2+2 photocycloaddition is obtained.