Patent ID: 8846322
Filing Date: 2014-09-30
Classification: C07K,G01N,Y10T

Abstract:
1. A method for isolating a target cell from a biological sample, the method comprising: a) contacting a particle comprising at least one polymer having negative charges and at least one antibody bound to the polymer with a biological sample in a solution to provide a mixed solution, wherein the antibody specifically binds to a surface marker of at least one target cell; b) adding a particle comprising at least one polymer having positive charges, or a particle comprising at least one polymer having positive charges and at least one antibody bound to the polymer, into the mixed solution of step a); c) adjusting a pH value of the mixed solution of step b); and d) isolating the target cell from the mixed solution of step c); or a) contacting a particle comprising at least one polymer having positive charges and at least one antibody bound to the polymer with a biological sample in a solution to provide a mixed solution, wherein the antibody specifically binds to a surface marker of at least one target cell; b) adding a particle comprising at least one polymer having negative charges, or a particle comprising at least one polymer having negative charges and at least one antibody bound to the polymer, into the mixed solution of step a); c) adjusting a pH value of the mixed solution of step b); and d) isolating the target cell from the mixed solution of step c).