Patent ID: 8058030
Filing Date: 2011-11-15
Classification: C12Q

Abstract:
1. A method for determining a polynucleotide sequence comprising: a) annealing a plurality of primers to a plurality of template polynucleotide sequences; b) extending the plurality of primers in the presence of one or more nucleoside triphosphates wherein at least one of the nucleoside triphosphates is modified, thereby producing a plurality of extension products that comprise a modified nucleotide sequence having one or more phosphorothiolate linkages; c) cleaving the phosphorothiolate linkages in the extension products, under conditions in which a plurality of fragments are produced; d) identifying from among the fragments produced in c), the fragments which comprise the primer; and e) identifying the nucleotide at the 3′ end of the fragments identified in d), thereby determining a polynucleotide sequence.