Patent ID: 9212386
Filing Date: 2015-12-15
Classification: C12Q,G01N

Abstract:
1. A lateral flow assay system for detecting a target enzyme in a sample, comprising: a) a reaction receptacle comprising: b) a sample analysis device comprising: wherein, when the target enzyme is present in the sample, the enzyme cleaves the peptide at the enzyme cleavage site such that the peptide can pass through the filter and be transferred from the reaction receptacle to the sample application zone of the sample analysis device, and the first tag binds to the immobilized second tag in the test zone such that the label is detectable in the test zone; and wherein, when the target enzyme is not present in the sample, the peptide is trapped in the reaction receptacle by the filter and is not transferred to the sample analysis device, such that there is no detectable signal in the test zone when an assay has been run.