Patent ID: 7599732
Filing Date: 2009-10-06
Classification: A61B,G01N

Abstract:
1. A method of imaging, comprising: directing time-varying excitation light at a surface area of a tissue, the tissue comprising a fluorescent target; modulating the intensity of the excitation light at a radio frequency; detecting, substantially at a two-dimensional sensor surface, time-varying emission light from the fluorescent target in response to the time-varying excitation light stimulating the fluorescent target; filtering the time-varying emission light to reject excitation light re-emitted from the material; determining a spatial distribution of amplitude and phase-delay of the detected time-varying emission light; detecting time-varying excitation light specularly-reflected by the fluorescent target; determining a spatial distribution of amplitude and phase-delay of the time-varying excitation light incident on the surface area based on the reflected time-varying excitation light; and generating a three-dimensional image of the fluorescent target based on the spatial distributions of the incident time-varying excitation light and the time-varying emission light.