Patent ID: 7256299
Filing Date: 2007-08-14
Classification: C07H,C09B,Y10S

Abstract:
1. A chemiluminescent reagent having the structure: wherein Q comprises a cycloalkyl or polycycloalkyl group attached covalently to the 4-membered ring portion of said dioxetane above directly or indirectly through a linkage group; wherein Z comprises hydrogen, alkyl, aryl, aralkyl, alkaryl, heteroalkyl, heteroaryl, cycloalkyl or cycloheteroalkyl; and wherein R 1 and R 2 comprise chemical moieties attached to different sites of a cyclic ring attached to said dioxetane, and wherein R 1 is enzymatically converted into R 1 * which comprises a chemical reactive group G 1 , and wherein R 2 is attached to said cyclic ring through an oxygen atom and comprises a chemical reactive group G 2 which reacts with said G 1 to convert said dioxetane to an unstable light-emitting dioxetane form.