Patent ID: 6465219
Filing Date: 2002-10-15
Classification: C12Q

Abstract:
A method for preparing a selected polynucleotide pool from a polynucleotide sample comprising:a) synthesizing first antisense polynucleotide strands from sense polynucleotides of, or prepared from, the polynucleotide sample, wherein said first antisense polynucleotide strands are synthesized using an antisense primer complex comprising an antisense primer operably linked to an RNA promoter sequence, wherein the RNA promoter sequence is 5â€² of the antisense primer; b) adding a universal primer site to the 3â€² ends of the first antisense polynucleotide strands; c) diluting the first antisense polynucleotide strands to substantially eliminate at least some low-abundance first antisense polynucleotide strands; and d) producing first double-stranded polynucleotides from the remaining first antisense polynucleotide strands, wherein the first double-stranded polynucleotides are enriched in high-abundance polynucleotide sequences relative to the polynucleotide sample.