Patent ID: 6569677
Filing Date: 2003-05-27
Classification: A61K,C07K,C12N

Abstract:
A method for producing an adenovirus whose genome lacks all or part of the sequences encoding a fiber, comprising:transfecting the genome of said adenovirus into a cell line comprising, either in a form integrated into the genome or in the form of an episome, a DNA fragment or expression vector encoding an adenovirus fiber modified by substitution mutation of one or more residues of a native fiber, wherein said native fiber residues are directed toward the natural cellular receptor recognized by the native adenovirus fiber, and wherein said residues are between the CD loop and the &bgr; sheet I of said fiber, the modification not including deletion of the entire knob region, said DNA fragment or expression vector placed under the control of the elements allowing its expression in said cell line, culturing said transfected cell line under appropriate conditions in order to allow the production of said adenovirus, and recovering said adenovirus from the culture of said transfected cell line.