Patent ID: 6156555
Filing Date: 2000-12-05
Classification: C07K,C12N,C12Y,Y10S

Abstract:
A method of preparing an enzyme, wherein said enzyme participates in C-terminal amidation and acts on a peptide C-terminal glycine adduct represented by the following formula (I): ##STR12## wherein A represents a residue other than .alpha.-amino group or imino group and .alpha.-carboxylic group derived from naturally occurring .alpha.-amino acid, X represents a hydrogen atom or a residue of an amino acid derivative which is bonded to the N atom through carbonyl group, to form a peptide C-terminal .alpha.-hydroxylglycine adduct represented by the following formula (II): ##STR13## wherein A and X have the meanings as above, but said enzyme does not convert the peptide C-terminal .alpha.-hydroxyglycine adduct (II) to a C-terminal amidated peptide represented by the following formula (III): ##STR14## wherein A and X have the meanings as above, wherein said method of preparing said enzyme comprises culturing host cells transformed with a plasmid comprising a cDNA which encodes said enzyme and which is capable of expressing said enzyme, and collecting said enzyme from the culture.