Patent ID: 7709209
Filing Date: 2010-05-04
Classification: C07K

Abstract:
1. In a method of separating antibodies from proteases, which method comprises (a) contacting a liquid comprising antibodies and protease(s) with a separation matrix to adsorb antibodies; (b) separating the liquid comprising protease(s) from the matrix; and optionally, (c) releasing the antibodies from the matrix by adding an eluent; the improvement being that the separation matrix comprises alkali-stable protein A-based ligands which provide increased resistance of said alkali-stable protein A-based ligands to protease degradation, wherein each of said alkali-stable protein A-based ligands is represented by SEQ ID NO: 1 or SEQ ID NO: 2, with one or two asparagine residues mutated, and the mutation is N23T; N23T, N43E; N28A; N6A; N11S; N11S, N23T; or N6A, N23T, further wherein said antibodies being separated are selected from the group consisting of IgG, IgA and IgM.