Patent ID: 6582936
Filing Date: 2003-06-24
Classification: C12N,C12Q

Abstract:
A method for making a nucleic acid comprising the step of:converting a primed single-stranded DNA to a double-stranded DNA by a method comprising the step of contacting the single-stranded DNA with a DNA polymerase having 5â€² exonuclease activity under conditions whereby the DNA polymerase converts the single-stranded DNA to the double-stranded DNA, wherein the 5â€² end of the single-stranded DNA comprises an RNA polymerase promoter joined to an upstream (5â€²) flaking moiety which protects the promoter from the 5â€² exonuclease activity of the DNA polymerase, wherein the upstream flanking moiety is selected from the group consisting of a dye, nucleotides âˆ’50 to âˆ’16 of the T7 promoter, modified nucleotide, a sterol, and an affinity tag.