Patent ID: 7314746
Filing Date: 2008-01-01
Classification: B03D,C12N,C12Q

Abstract:
1. A method of producing a clarified cell lysate comprising plasmid DNA from an alkaline bacterial cell lysate, comprising the steps of: introducing a suspension of bacterial cells into a fluid flow comprising an alkaline lysis buffer and an entrainment of gas, wherein the cells are flowably mixed with the cell lysis buffer together with the gas thereby forming a cell lysis mixture; introducing a precipitation buffer into the fluid flow comprising the cell lysis mixture, thereby forming a precipitated lysate; introducing a pH adjustment buffer into the precipitated lysate and combining the pH adjustment buffer and the precipitated lysate prior to separating the mixture; separating the mixture into a buoyant flocculent phase comprising precipitated cell debris and a fluid phase comprising a substantially clarified cell lysate; and isolating the clarified cell lysate.