Patent ID: 8610086
Filing Date: 2013-12-17
Classification: G01N,G02B

Abstract:
1. Method for spatially high-resolution luminescence microscopy of a sample which is labelled with label molecules which can be activated by a switching signal such that the label molecules can be excited to emit particular luminescence radiation only in the activated state, comprising: a) introducing the switching signal onto the sample such that only a subset of the label molecules present in the sample are activated, wherein there are area parts in the sample in which activated label molecules have a distance to their closest neighbouring activated label molecules that is at least greater than or equal to a length which results from a predetermined optical resolution, b) exciting the activated molecules to emit luminescence radiation, c) detecting the luminescence radiation with the predetermined optical resolution, d) generating and acquisitioning a frame from the luminescence radiation recorded in step c), wherein the geometric locations of the label molecules emitting luminescence radiation are identified with a spatial resolution increased above the predetermined optical resolution, wherein the steps are repeated several times and the several, thus-obtained frames are combined into a total image, and controlling the acquisition of the several frames by evaluating at least one of the frames or a group of the frames after step d) and modifying at least one variable of steps a-d for subsequent repetitions of steps a-d.