Patent ID: 7575887
Filing Date: 2009-08-18
Classification: C12Q,G01N

Abstract:
1. A method for detecting the presence of a protease within a test sample, the method comprising: i) providing a lateral flow assay device that comprises a fluidic medium, the lateral flow assay device comprising a sample application zone, the fluidic medium defining a detection zone within which is immobilized a first reagent, the detection zone being in fluid communication with a substrate, wherein the substrate is capable of cleavage by the protease and a chromogenic product is released upon the cleavage of the substrate by the protease; ii) contacting the sample application zone with the test sample, wherein the test sample travels through the fluidic medium from the sample application zone to the detection zone following contacting of the sample application zone with the test sample; and iii) generating a color within the detection zone that corresponds to the presence or absence of the protease in the test sample.