Patent ID: 7402390
Filing Date: 2008-07-22
Classification: B01D,B01J,C12N,C12Q

Abstract:
1. A method of isolating nucleic acid from a sample containing nucleic acid, the method comprising: contacting the sample with a bifunctional material that contains an amino group and a carboxyl group and is positively charged at a first pH to allow binding of nucleic acid to the bifunctional material to form a nucleic acid-bifunctional material complex; and extracting the bound nucleic acid at a second pH which is higher than the first pH from the complex, wherein the bifunctional material is represented by the following Formula 1 or Formula 2: where n is an integer from 1 to 10; and A is or a combination, from 1,2,4,5-benzenetetracarboxylic dianhydride; from 3,3′,4,4′-biphenyltetracarboxylic dianhydride, 2,2′,3,3′-biphenyltetracarboxylic dianhydride, or 2,3,3′,4′-biphenyltetracarboxylic dianhydride; or a combination, from bis(3,4-dicarboxyphenyl)sulfone dianhydride; or a combination, from 2,2-bis(3,4-dicarboxyphenyl)-hexafluoropropane dianhydride; or a combination, from bis(3,4-dicarboxyphenyl) ether dianhydride; from 3,3′,4,4′-benzophenonetetracarboxylic dianhydride, 2,2′,3,3′-benzophenonetetracarboxylic dianhydride or 2,3,3′,4′-benzophenonetetracarboxylic dianhydride; from 1,4,5,8-naphthalenetetracarboxylic dianhydride, 1,2,5,6-naphthalenetetracarboxylic dianhydride, or 2,3,6,7-naphthalenetetracarboxylic dianhydride, wherein the naphthalene is further unsubstituted or a carbonyl group and a carboxyl group is substituted on any carbon position except for a linking portion of the rings; from cyclobutanetetracarboxylic dianhydride; from methylcyclobutanetetracarboxylic dianhydride; or from 1,2,3,4-tetracarboxybutane dianhydride, wherein moiety A is a group generated from a reaction between the corresponding tetracarboxylic dianhydride, and an amino group of an NH 2 (CH 2 ) n NH 2 where n is an integer from 1 to 10; and where R 1 and R 2 are each independently selected from the group consisting of an —OH group and an —NH(CH 2 ) n NH 2 group, where n is an integer from 1 to 10; R 3 is an alkyl group having 1 to 10 carbon atoms; and 1 is an integer from 1 to 30,000.