Patent ID: 6353098
Filing Date: 2002-03-05
Classification: A61K,C07K,C12N,C12Q,Y02P

Abstract:
A process for deprotecting and purifying chemically synthesized RNA, comprising the steps of:(a) deprotection, wherein said deprotection comprises the step of removing any exocyclic protecting groups from said RNA molecule by contacting said RNA with an alkylamine or a mixture of ammonium hydroxide and alkylamine at a temperature of about 60-70 degrees centigrade for about 5-15 minutes, under conditions suitable for the removal of said exocyclic protecting groups, wherein said alkylamine is selected from the group consisting of ethylamine, propylamine and butylamine; and (b) purification, wherein said purification comprises passing the chemically synthesized RNA over a high performance liquid chromatography (HPLC) column or an anion exchange column or both, under conditions which allow for the purification of the RNA.