Patent ID: 7057014
Filing Date: 2006-06-06
Classification: C07K

Abstract:
1. A process of removing residual organic solvent from an otherwise pure nona- or decapeptide, comprising the following steps: dissolving the nona- or decapeptide in a dissolution solvent mixture comprising water and at least one alcohol selected from a group consisting of methanol, ethanol, propanol, isopropanol; adding the solution of the nona- or decapeptide in said solvent mixture to a vigorously stirred precipitation solvent mixture consisting essentially of one or several polar compounds selected from a group consisting of methyl acetate, ethyl acetate, methyl propionate, ethyl propionate, butyl acetate, isobutyl acetate, t-butyl acetate, ethyl formate, propyl formate and isopropyl formate, and one or several non-polar compounds selected from a group consisting of hexane, heptane, octane, cyclohexane and methylcyclohexane, and, optionally of up to 5% of acetic or propionic acid; isolating the precipitated nona- or decapeptide; washing the isolated nona- or decapeptide with one or a mixture of said polar compounds or a solvent or solvent mixture of similar polarity, drying the washed nona- or decapeptide, with the proviso that the water content of said solvent mixture comprising water and at least one alcohol is below 8% (v/v), and that the volume ratio of the dissolution solvent mixture and the precipitation solvent mixture is 1:10 or more.