Patent ID: 6177558
Filing Date: 2001-01-23
Classification: B01J,B82Y,C07H,C40B,Y10S

Abstract:
A method of reducing the evaporation of a liquid reagent solution during solid phase chemical synthesis of molecules selected from the group consisting of nucleic acids, peptides and peptide nucleic acids on an open environment solid support surface, said method comprising the steps of:(a) providing an open solid support surface including at least one binding site which is functionalized with a chemical moiety; and(b) depositing a minute volume of liquid reagent solution onto said support surface, and in contact with said binding site, said reagent solution including reactants contained in at least one polar, aprotic solvent having a boiling point of at least about 140.degree. C. and selected from the group consisting of dinitriles, mononitriles, glymes, diglymes, triglymes, and trimethylphosphates, to reduce evaporation of the reagent solution in the open environment during synthesis on said solid support.