Patent ID: 6703213
Filing Date: 2004-03-09
Classification: A61K,C07H,C12N,Y10S

Abstract:
A method of detecting GlcNAc transferase activity in a sample suspected of containing a protein or an active fragment thereof exhibiting GlcNAc transferase activity comprising:contacting a sample suspected of containing a protein or an active fragment thereof exhibiting GlcNAc transferase activity with effective amounts of labeled UDP-GlcNAc substrate and a substance comprising the chemical moiety of the formula: â€ƒin which â€œRâ€  is an acyl group comprising 2 or more carbon atoms, â€œR1â€  is a substituted or unsubstituted alkyl group comprising 1 or more carbon atoms, â€œR2â€  is hydrogen or a substituted or unsubstituted alkyl group comprising 1 or more carbon atoms, â€œAâ€  is a substituted or unsubstituted amino acid residue or a peptide comprising 2 or more substituted or unsubstituted amino acid residues, â€œR3â€  comprises a substituted or unsubstituted alkyl group comprising 10 to 40 carbon atoms, pyrophosphate protecting groups and pharmaceutically acceptable salts thereof, provided that said substance is not Lipid I, the natural substrate of wild type MurG enzyme, under conditions effective to provide a labeled coupling product comprising labeled GlcNAc coupled to said substance via a glycosidic bond in the presence of a protein or an active fragment thereof exhibiting GlcNAc transferase activity; detecting the formation or presence of said labeled coupling product, which is indicative of GlcNAc transferase activity in said sample.