Patent ID: 8062860
Filing Date: 2011-11-22
Classification: G01N,Y10S,Y10T

Abstract:
1. A kit for assessing the mutagenic DNA-damaging potential of an agent comprising: a first reagent that binds glycosylphosphatidylinositol (GPI1-anchor-expressing cells, but not GPI-anchor-deficient cells, and comprises a first fluorochrome; a second reagent that binds to mammalian platelets, and that comprises a second fluorochrome having a fluorescent emission spectrum that does not substantially overlap with a fluorescent emission spectrum of the first fluorochrome; a third fluorochrome-containing reagent that labels mature erythrocytes but not reticulocytes, the third fluorochrome having a fluorescent emission spectrum that does not substantially overlap with the fluorescent emission spectra of the first and second fluorochromes; optionally, one or more balanced salt solutions; and a computer readable storage medium comprising a cytometry data acquisition template that acquires from a flow cytometer scoring data for GPI-anchor-deficient erythrocytes and reticulocytes, which exhibit cell-specific forward- and side-scatter properties and the absence of fluorescence from the first reagent, and are distinguished from one another and from nucleated cells by fluorescence levels for the third reagent and distinguished from platelets that exhibit fluorescence of the second reagent as a basis to execute assessment of the mutagenic DNA-damaging potential of the agent.