Patent ID: 8114637
Filing Date: 2012-02-14
Classification: C07K,C12N

Abstract:
1. A method for generating a batch of recombinant adenovirus having a deletion in the adenovirus' E1 region, the method comprising: a) introducing a recombinant adenovirus or recombinant adenovirus' genome into a cell comprising adenovirus E1 nucleic acid sequences able to complement the deletion of the adenovirus' E1 region of the recombinant adenovirus, wherein the cell comprises an adenovirus E1 A coding sequence, an adenovirus E1B-19K coding sequence, and an adenovirus E1B-55K coding sequence integrated into the cell's genome, wherein the cell has been prepared by a process comprising: b) culturing the cell; and c) harvesting the batch of recombinant adenovirus.