Patent ID: 6235971
Filing Date: 2001-05-22
Classification: C12N

Abstract:
A method of producing a transgenic plant, comprising the steps of:(a) stably transforming into the genome of a plant cell a recombinant, double-stranded DNA molecule comprising:(i) a promoter which functions in cells of target plant tissue,(ii) a structural DNA sequence obtained from a bacterium of the genus Leuconostoc that causes the production of an RNA sequence which encodes a sucrose phosphorylase enzyme,(iii) a 3' non-translated DNA sequence which functions in plant cells to cause transcriptional termination and the addition of polyadenylated nucleotides to the 3' end of the RNA sequence;(b) selecting for transformed plant cells; and(c) regenerating from said transformed plant cells a genetically transformed plant, the genome of which contains said recombinant, double-stranded DNA molecule of step (a), wherein said genetically transformed plant exhibits a property selected from the group consisting of containing a modified carbohydrate content; increased polysaccharide content; enhanced yield of harvested material; improved uniformity of the distribution of solids; and reduced susceptibility to bruising discoloration.