Patent ID: 6346399
Filing Date: 2002-02-12
Classification: C12N,C12Q,Y10S,Y10T

Abstract:
A method for isolating DNA subsets from a first sample and a second sample for use in genomic mismatch analysis, comprising:(a) subjecting first portions of said first and second samples separately to a first oligonucleotide consisting of at least two copies of any dinucleotide, trinucleotide, or tetranucleotide repeat attached to a support to obtain first and second sample fractions that bind to the first oligonucleotide; (b) subjecting second portions of said first and second samples separately to a second oligonucleotide consisting of at least two adjacent copies of any dinucleotide, trinucleotide or tetranucleotide repeat different than that employed in step (a) attached to a support to obtain first and second sample fractions that bind to the second oligonucleotide; (c) mixing the first sample fraction obtained in step (a) with the second sample fraction obtained in step (b), and mixing the second sample fraction obtained in step (a) with the first sample fraction obtained in step (b); (d) denaturing and reannealing each mixture to form heterohybrids, wherein the heterohybrids comprise the DNA subsets to be used in genomic mismatch analysis.