Patent ID: 6818112
Filing Date: 2004-11-16
Classification: G01N,Y10T

Abstract:
A method for separating a plurality of proteins comprising performing a capillary electrophoretic method with a sample containing the plurality of proteins, wherein said electrophoretic method comprises reducing electroosmotic flow (EOF) within a capillary utilized to conduct the capillary electrophoretic method by a plurality of methods, wherein at least one of the reduction methods comprises introducing the sample downstream of a porous plug positioned within the capillary, and wherein one or more of the other reduction methods comprise generating an induced counter current to counteract EOF within the capillary, performing the electrophoretic method with a capillary that has an internal surface coated with an agent effective to reduce EOF, and/or performing the electrophoretic method with a separation medium containing an agent that coats an internal surface of the capillary.