Patent ID: 6509167
Filing Date: 2003-01-21
Classification: A61K,C12Q,G01N

Abstract:
A method of identifying a candidate agent as a modulator of function of a target protein complex wherein said target protein complex comprises a biochemically functional sarcomere and said method comprises:a) adding in vitro a candidate agent to a mixture comprising a target protein complex that directly or indirectly produces ADP or phosphate under conditions which normally allow the production of ADP or phosphate, wherein said target protein complex comprises isolated myosin and isolated actin; b) subjecting the mixture to an enzymatic reaction that uses said ADP or phosphate as a substrate under conditions which normally allow the ADP or phosphate to be utilized; and determining the level of activity of the enzymatic reaction wherein a change in said level between the presence and absence of said candidate agent indicates that said candidate agent is a modulator of said target protein function.