Patent ID: 7718763
Filing Date: 2010-05-18
Classification: C07K,C12N,C12Q

Abstract:
1. An isolated substrate polypeptide for a disintegrin-like and metalloprotease with thrombospondin type-1 motif, 13 (ADAMTS-13), and an optionally included covalently attached heterologous tag, wherein the isolated substrate polypeptide consists of amino acids 1587 to 1668 of the amino acid sequence of wild-type human von Willebrand factor (VWF) in SEQ ID NO: 1, wherein the tag is attached at the N-terminal and/or at the C-terminal of said polypeptide and said tag is selected from the group consisting of a glutathione transferase (GST) luciferase, beta-galactosidase, His tag peptides, coupling agents, radioactive labels, and chromophores.