Patent ID: 6093562
Filing Date: 2000-07-25
Classification: C11D,C12N,C12Y,D06L

Abstract:
A variant of a parent alpha-amylase enzyme having an increased thermostability or a decreased isoelectric point as compared to the parent enzyme, wherein said parent alpha-amylase has an amino acid sequence having at least an 80% homology to SEQ ID No. 1, SEQ ID No. 2, SEO ID No. 3, and SEQ ID No. 7 and wherein said variant comprises an amino acid substitution which is, or which is equivalent to, one of the following substitutions in the amino acid sequence shown in SEQ ID NO. 1: K269R, P260E, R124P, L217I, Y243F, V206I, L, F, L351C, Q391E, K444Q, N457D, N457E, Q86E, Q346E and Q391E.