Patent ID: 8951771
Filing Date: 2015-02-10
Classification: C12N,C12P

Abstract:
1. A modified 13-hydroperoxide lyase polypeptide comprising an amino acid sequence which has no more than 40 amino acid alterations as compared to the amino acid sequence of the wild-type protein having the amino acid sequence of SEQ ID NO. 1 wherein at least one amino acid alteration position is selected from the group consisting of positions 3, 4, 5, 19, 208, 340, 342, 352, 354, 358, 359, 360, 371, 372, 375, 377, 382, 383, 387, 388, 389, 392, 393, 394, 395, 399 and 457 , with said modified 13-hydroperoxide lyase having increased enzymatic activity as compared to the wild-type protein having the amino acid sequence of SEQ ID NO. 1 and wherein the modified lyase comprises one or more substitutions selected from the group consisting of V3L, R4K, TSP, 519L, N208H, F340L, Y342F, K352R, K352S, H354Y, F358Y, D359E, V360l, K371 P, V372L, T375R, P377S, E382D, P383A, N387K, S388A, D389E, V392M, Q393G, N394E, D399S, D399N and N457K wherein the modified polypeptide has the amino-terminal amino acid sequence ATPSSSSPE (SEQ ID NO:18).