Patent ID: 7285699
Filing Date: 2007-10-23
Classification: A01K,C12N

Abstract:
1. A method of gene targeting in Drosophila comprising: (a) selecting a (b) providing a donor construct comprising a transposon and a DNA segment homologous to the target gene comprising a marker sequence, wherein the DNA segment is flanked at both ends by a rare-cutting endonuclease recognition site and additionally flanked by a recombinase recognition site; (c) introducing the donor construct to a female (d) providing a construct comprising a DNA sequence encoding a rare-cutting endonuclease and a DNA sequence encoding a recombinase, wherein each DNA sequence is operably linked to an inducible promoter; (e) introducing the construct of step (d) to a male (f) crossing the male transgenic (g) activating the inducible promoter in the progeny of step (f) to express the rare-cutting endonuclease and the recombinase such that recombination occurs between the target gene and the target construct; and (h) selecting a transgenic progeny whose genome comprises the disrupted target gene.