Patent ID: 7932353
Filing Date: 2011-04-26
Classification: A61K,A61P,C07K

Abstract:
1. A method of producing a collagen-like polypeptide comprising: (a) replacing any threonine in a naturally occurring collagen polypeptide with any other amino acid; and/or (b) introducing a glycine adjacent to the N-terminal side of each threonine in the naturally occurring collagen polypeptide; and/or (c) introducing a proline adjacent to the C-terminal side of each threonine the naturally occurring collagen; and (d) removing all of the Asn-X-Thr sites wherein X can be any amino acid; and (e) replacing any serine in a naturally occurring collagen polypeptide with any other amino acid; and (f) expressing the collagen-like polypeptide in a micro-organism such that the collagen-like polypeptide is not glycosylated and not phosphorylated and wherein the micro-organism is free of active post-translational hydroxylation of proline and lysine.