Patent ID: 8357672
Filing Date: 2013-01-22
Classification: C07H,C12N

Abstract:
1. A reagent for extracting RNA from cellular material, said reagent comprising: (a) from about 1 pg/μL to about 10 pg/μL heparin; (b) from about 1 mM to about 10 mM of a reducing agent effective to reduce disulfide bonds in cellular proteins; (c) a chelating agent in an amount sufficient to disrupt RNA secondary and tertiary structures; (d) from about 1 mM to about 100 mM of a buffer selected from the group consisting of Tris base, Tris-HCl, Bis-Tris base, Bis-Tris-HCl, and HEPES; and (e) from about 1 mM to about 100 mM of an alkali metal halide; said reagent being devoid of chlorinated solvents.