Patent ID: 9006410
Filing Date: 2015-04-14
Classification: A61K,A61P,C07K,C12N,C12P

Abstract:
1. A nucleic acid construct for expression of at least one biologically active fusion glycoprotein in plants comprising: a) at least one nucleic acid sequence encoding a plant hydroxyproline O-glycosylation glycomodule of (X-Hyp) n (SEQ ID NO: 3), where n is from 10 to 25; and wherein X is chosen from Ser, Ala, Thr, and Val, b) at least one operatively linked nucleic acid sequence encoding a biologically active mammalian protein selected from human growth hormone and human interferon, and c) an operatively linked plant promoter; wherein the at least one biologically active fusion protein encoded by the nucleic acid construct exhibits, when expressed in plants, a pharmacokinectic half-life in plasma that is increased by at least about 500% as compared with the same biologically active mammalian protein that lacks the at least one plant hydroxyproline O-glycosylation glycomodule; wherein the at least one biologically active fusion protein encoded by the nucleic acid construct is, when expressed in plants, hydroxyproline O-glycosylated at the (X-Hyp) wherein the plant hydroxyproline O-glycosylation glycomodule of (X-Hyp)