Patent ID: 6051425
Filing Date: 2000-04-18
Classification: C12N,Y10T

Abstract:
A method for producing a tissue formation having a structure differentiated in relation to depth, comprising the steps of:inoculating a first culturing cell on a first bio-compatible sponge having first pores, said first pores having a size such that said first culturing cell is accommodated in said first pores to allow said first culturing cell to proliferate inside said first bio-compatible sponge;inoculating a second culturing cell on a second bio-compatible sponge having second pores, said second pores having a size such that said second culturing cell is accommodated on said second bio-compatible sponge where inoculated to allow said second culturing cell to proliferate on said second bio-compatible sponge, said second bio-compatible sponge having a lower degree of resistance against collagenase secreted by said second culturing cell than that of said first bio-compatible sponge against said first culturing cell; andculturing said first and second culturing cells using at least one culture medium to produce a tissue formation having a structure differentiated in relation to depth.