Patent ID: 6127145
Filing Date: 2000-10-03
Classification: C12N

Abstract:
A method for producing a stable, glycosylated .alpha..sub.1 -antitrypsin (AAT) comprising the steps of:(a) obtaining rice or barley cells transformed with a chimeric gene having (i) an inducible transcriptional regulatory region, (ii) a first DNA sequence encoding AAT, and (iii) a second DNA sequence encoding a signal polypeptide, where said second DNA sequence is operably linked to said transcriptional regulatory region and said first DNA sequence, and where said signal polypeptide is in translation-frame with said AAT and is effective to facilitate secretion of expressed AAT from the transformed cells,(b) cultivating the transformed cells under conditions effective to induce the promoter, thereby to promote expression of AAT and secretion of the protein from the transformed cells; and(c) isolating AAT produced by the cells.