Patent ID: 6653071
Filing Date: 2003-11-25
Classification: C07K,G01N

Abstract:
A method of identifying a gene as a member of a regulatory pathway of a component of general transcription machinery in a cell, comprising the steps of:(a) hybridizing a transcription indicator of a test cell to a set of nucleic acid probes; (b) hybridizing a transcription indicator of a control cell to the set of nucleic acid probes, wherein the transcription indicators are selected from the group consisting of mRNA, cDNA and cRNA, wherein the test cell contains a mutant component of the general transcription machinery and the control cell is a wild-type isogenic counterpart of the test cell;(c) detecting amounts of the transcription indicators which hybridize to each of said set of nucleic acid probes; and (d) identifying a gene as a member of the regulatory pathway of the component of the general transcription machinery, if the amount of transcription indicator of the test cell which hybridizes to a nucleic acid probe comprising a portion of the gene is different from the amount of transcription indicator of the control cell which hybridizes to a nucleic acid probe comprising a portion of the gene.