Patent ID: 7094606
Filing Date: 2006-08-22
Classification: C07K,C12N

Abstract:
1. A method of making a localized mutation in a plant cell to an ALS gene causing the plant cell to be herbicide resistant comprising the steps of: (a) adhering to a particle a recombinagenic oligonucleobase, which contains a first homologous region which has a sequence identical to the sequence of at least 6 base pairs of a first fragment of the ALS gene and a second homologous region which has a sequence identical to the sequence of at least 6 base pairs of a second fragment of the ALS gene, and an intervening region which contains at least 1 nucleobase heterologous to the ALS gene, which intervening region connects the first homologous region and the second homologous region; (b) introducing the particle into a cell of a population of plant cells; (c) identifying a cell of the population of plant cells having a mutation located between the first and second fragments of the ALS gene.