Patent ID: 6794127
Filing Date: 2004-09-21
Classification: B01J,B01L,B82Y,C12N,C12Q,C40B

Abstract:
A method for identifying an enzymatic activity of interest, comprising:(a) introducing a fluorogenic or chromogenic substrate and a recombinant clone into a capillary tube of a capillary array, wherein each capillary tube of the capillary array comprises at least one wall defining a lumen for retaining the substrate and the recombinant clone, and wherein the at least one wall is comprised of a material having a low refractive index; (b) culturing the clone in the capillary tube containing the substrate and the recombinant clone under conditions which allow interaction of the substrate and an enzyme produced by the recombinant clone to produce a detectable optical signal; and (c) detecting the detectable optical signal in the capillary tube to identify one or more capillaries containing the detectable optical signal, thereby identifying the enzymatic activity of interest.