Patent ID: 7425625
Filing Date: 2008-09-16
Classification: C07H,C12N,C12P

Abstract:
1. An isolated nucleic acid molecule encoding a polypeptide having carotenoid ketolase activity, the polypeptide having the amino acid sequence set forth in SEQ ID NO:2, wherein the polypeptide has an amino acid substitution selected from the group consisting of: a) a replacement of threonine at amino acid position 121 of SEQ ID NO:2 with alanine; b) a replacement of methionine at amino acid position 142 of SEQ ID NO:2 with leucine; c) a replacement of alanine at amino acid position 164 of SEQ ID NO:2 with valine; d) a replacement of isoleucine at amino acid position 283 of SEQ ID NO:2 with valine; e) a replacement of threonine at amino acid position 304 of SEQ ID NO:2 with lysine; f) a replacement of arginine at amino acid position 339 of SEQ ID NO:2 with glutamine; g) a replacement of arginine at amino acid position 519 of SEQ ID NO:2 with tryptophan; h) a replacement of glutamine at amino acid position 524 of SEQ ID NO:2 with leucine or arginine; and i) a combination thereof.