Patent ID: 6350444
Filing Date: 2002-02-26
Classification: A61K,A61P,C12N,C12Y

Abstract:
A method for increasing pulmonary edema clearance in a diseased mammalian lung, said method comprising:(a) obtaining a replication deficient adenovirus genetic vector comprising (i) an adenovirus genome that is deleted for all nucleotide sequences encoding adenovirus proteins; and (ii) a nucleotide sequence encoding a single Na,K-ATPase subunit; and (b) transferring the adenoviral genetic vector into epithelial cells of the lung via aerosol deposition or direct endotracheal instillation so as to express the nucleotide sequence encoding the single Na,K-ATPase subunit at levels that are an overexpression compared to levels in lung cells not having the genetic vector, whereby said pulmonary edema clearance of the mammalian lung is increased.