Patent ID: 7972782
Filing Date: 2011-07-05
Classification: A01K,A61K,C07K,C12N,C12Q,G01N

Abstract:
1. A method for screening a collection of compounds to determine those compounds which bind to an SXR polypeptide, said method comprising employing an SXR polypeptide in a binding assay, wherein said SXR polypeptide is a member of the steroid/thyroid superfamily and is further characterized by: forming a heterodimer with retinoid X receptor (RXR), wherein said heterodimer binds to a direct or inverted repeat response element comprising at least two half sites RGBNNM separated by a spacer of 0 up to 15 nucleotides, wherein: with the proviso that at least 4 nucleotides of said —RGBNNM- sequence are identical with the nucleotides at corresponding positions of the sequence AGTTCA, activating transcription of a gene under the control of a cytochrome P450 response element in response to a wide variety of natural and synthetic steroid hormones, at least including compounds that induce catabolic enzymes, steroid receptor agonists and antagonists, and bioactive dietary compounds, and being detectably expressed in the liver and the intestine.