Patent ID: 8067203
Filing Date: 2011-11-29
Classification: A61K,A61P,C07K

Abstract:
1. A method of making a composition, which comprises: (a) separately cloning the DNA fragment coding Pasteurella multocida toxin (rPMT) subunits with amino acid residues 1-487 in SEQ ID NO: 2 into pET32b expression vector, cloning the DNA fragment coding Pasteurella multocida toxin (rPMT) subunits with amino acid residues 485-987 in SEQ ID NO: 2 into pET32a expression vector, and cloning the DNA fragment coding Pasteurella multocida toxin (rPMT) subunits with amino acid residues 986-1282 in SEQ ID NO: 2 into pET32b expression vector to obtain three recombinant plasmids; (b) transforming each of the recombinant plasmids into an E. coli BL21 (DE3) strain for the expression of the Pasteurella multocida toxin (rPMT) subunits Tox 1, Tox2, and Tox7; (c) harvesting and purifying the recombinant rPMT subunits from the transformant cell cultures; and (d) combining the purified recombinant rPMT subunits in an adjuvant.