Patent ID: 6773917
Filing Date: 2004-08-10
Classification: C12N,C12P

Abstract:
A transformed plant, a plastid of which comprises:(a) a polypeptide encoded by a nucleotide sequence encoding a branched chain oxoacid dehydrogenase complex E1&agr; subunit protein, said nucleotide sequence selected from the group consisting of: (i) the nucleotide sequence shown in SEQ ID NO:11, or the complement thereof; (ii) a nucleotide sequence that hybridizes to said nucleotide sequence of (i) under a wash stringency equivalent to 0.1Ã—SSC, 0.1% SDS, at 55Â° C., and which encodes a polypeptide having enzymatic activity differing from that of Arabidopsis thaliana branched chain 2-oxoacid dehydrogenase complex E1&agr; subunit by about 30% or less; (iii) a nucleotide sequence encoding the same amino acid sequence as said nucleotide sequence of (i), but which is degenerate in accordance with the degeneracy of the genetic code; and (iv) a nucleotide sequence encoding the same amino acid sequence as said nucleotide sequence of (ii), but which is degenerate in accordance with the degeneracy of the genetic code; (b) a polypeptide encoded by a nucleotide sequence encoding a branched chain oxoacid dehydrogenase complex E1&bgr; subunit protein, said nucleotide sequence selected from the group consisting of: (i) the nucleotide sequence shown in SEQ ID NO:13, or the complement thereof; (ii) a nucleotide sequence that hybridizes to said nucleotide sequence of (i) under a wash stringency equivalent to 0.1Ã—SSC, 0.1% SDS, at 55Â° C., and which encodes a polypeptide having enzymatic activity differing from that of Arabidopsis thaliana branched chain 2-oxoacid dehydrogenase complex E1&bgr; subunit by about 30% or less; (iii) a nucleotide sequence encoding the same amino acid sequence as said nucleotide sequence of (i), but which is degenerate in accordance with the degeneracy of the genetic code; and (iv) a nucleotide sequence encoding the same amino acid sequence as said nucleotide sequence of (ii), but which is degenerate in accordance with the degeneracy of the genetic code; (c) a polypeptide encoded by a nucleotide sequence encoding a branched chain oxoacid dehydrogenase complex E2 component protein, said nucleotide sequence selected from the group consisting of: (i) the nucleotide sequence shown in SEQ ID NO:15, or the complement thereof; (ii) a nucleotide sequence that hybridizes to said nucleotide sequence of (i) under a wash stringency equivalent to 0.1Ã—SSC, 0.1% SDS, at 55Â° C., and which encodes a polypeptide having enzymatic activity differing from that of Arabidopsis thaliana branched chain 2-oxoacid dehydrogenase complex E2 subunit by about 30% or less; (iii) a nucleotide sequence encoding the same amino acid sequence as said nucleotide sequence of (i), but which is degenerate in accordance with the degeneracy of the genetic code; and (iv) a nucleotide sequence encoding the same amino acid sequence as said nucleotide sequence of (ii), but which is degenerate in accordance with the degeneracy of the genetic code; and (d) an enzyme selected from the group consisting of aspartate kinase, homoserine dehydrogenase, threonine synthase, and threonine deaminase.