Patent ID: 8652460
Filing Date: 2014-02-18
Classification: A61P,C12N

Abstract:
1. A replication competent mammalian oncoretrovirus comprising: a nucleic acid sequence encoding a retroviral GAG protein; a nucleic acid sequence encoding a retroviral POL protein; a nucleic acid sequence encoding a retroviral envelope; a mammalian oncoretroviral polynucleotide sequence comprising Long-Terminal Repeat (LTR) sequences at the 5′ and 3′ end of the mammalian oncoretroviral polynucleotide sequence; a cassette comprising an internal ribosome entry site (IRES) operably linked to a heterologous nucleic acid sequence, wherein the cassette is positioned 5′ to the U3 region of the 3′ LTR, and just downstream and 3′ to the sequence encoding the retroviral envelope, wherein the heterologous nucleic acid sequence is expressed; and cis-acting sequences for reverse transcription, packaging and integration in a naturally occurring target mammalian cell.