Patent ID: 8778669
Filing Date: 2014-07-15
Classification: C12M

Abstract:
1. A vessel for culturing cells comprising: a bottom including a base with an upwardly extending wall at least partially bounding the base of the bottom; a top including a base with a downwardly extending wall at least partially bounding the base of the top; a tubular neck with an opening defined therein; and, at least one shelf, wherein, each shelf includes a base with an upwardly extending wall at least partially bounding the base of the shelf, wherein, wherein, wherein the at least one shelf includes a second shelf, the second shelf being located intermediate the bottom and the first shelf, the wall of the bottom adjoining the second shelf, wherein the vessel has spaced-apart first and second ends, the tubular neck extending from the second end, a first volume being defined between the top and the first shelf extending a predetermined length from the first end, the predetermined length being less than the spacing between the first and second ends, a second volume being defined between the bottom and the second shelf extending the predetermined length from the first end, the first and second volumes being substantially equal, wherein the opening is aligned to permit pipette access to the first volume in proximity to the first end of the vessel between the top and the first shelf, wherein a flow aperture is formed in each of the shelves in proximity to the first end of the vessel, the first volume being in communication with the flow aperture of the first shelf, the second volume being in communication with the flow aperture of the second shelf, wherein the base of the top having a first portion spaced a first distance from the base of the first shelf, the base of the top having a second portion spaced a second distance from the base of the first shelf, the first distance being greater than the second distance, and, wherein the second portion extends from the first end of the vessel the predetermined length.