Patent ID: 7981620
Filing Date: 2011-07-19
Classification: C07K,C12N,C40B,G01N

Abstract:
1. An isolated nucleic acid molecule encoding a protein comprising a fibronectin type III domain from the tenth module of the human fibronectin type III domain, wherein the fibronectin type III domain comprises at least two fibronectin type III β-strand domains with a loop region linked between each β-strand domain, wherein the integrin-binding domain has been removed from the FG loop of the fibronectin type III domain, wherein at least one loop region comprises an AB, BC, CD, DE, or the FG loop region sequence that is randomized or varies as compared to the wild-type fibronectin type III loop region (SEQ ID NO:110; FIG. 2 ) by deletion of two to twelve amino acids in the loop region, insertion of at least two to 25 amino acids in the loop region, or replacement of at least two amino acids in the loop region, wherein at least one loop region binds to a specific binding partner (SBP) to form a polypeptide:SBP complex.