Patent ID: 6887712
Filing Date: 2005-05-03
Classification: A61K,G01N,Y10S,Y10T

Abstract:
1. A method to determine if a compound causes a change in the structure of apolipoprotein B-100 in a cholesterol-containing low density lipoprotein thus increasing the binding of an epitope on the apolipoprotein B-100 to the LDL-receptor, comprising: (i) mixing the compound with and allowing it to bind to cholesterol-containing low density lipoprotein forming a complex; (ii) exposing the complex to a first capture antibody that is attached to a solid phase material and is directed to the epitope on apolipoprotein B-100 that binds to the LDL-receptor, forming a combination; (iii) adding a second antibody which binds to the combination; (iv) detecting the second antibody bound to the combination by the addition of a third antibody that binds the second antibody and to which is attached a label; (v) quantifying the amount of the captured complex by quantifying the amount of label; and (vi) comparing the amount of cholesterol-containing low density lipoprotein captured by the assay to a control, wherein an increase in the amount of cholesterol-containing low density lipoprotein captured indicates a change in the structure of apoB-100 in the cholesterol-containing low density lipoprotein, thus increasing binding to the low density lipoprotein receptor.