Patent ID: 6531286
Filing Date: 2003-03-11
Classification: C07H,C12Q,Y10T

Abstract:
A method for detecting the presence or absence of a target molecule in a test mixture which may contain said target molecule comprising:a) contacting the test mixture with a first nucleic acid ligand which binds to said target molecule, wherein said first nucleic acid ligand undergoes a conformational change; b) contacting the mixture produced by step a) with a second nucleic acid ligand which binds to the conformationally changed first nucleic acid ligand, wherein said second nucleic acid ligand undergoes a conformational change; c) contacting the mixture produced by step b) with a third nucleic acid ligand which binds to the conformationally changed second nucleic acid ligand, thereby forming a cascade hybridization complex; and d) detecting the presence of said cascade hybridization complex.