Patent ID: 7439057
Filing Date: 2008-10-21
Classification: C12M

Abstract:
1. A method for making an engineered tissue construct, comprising the steps of: providing a chamber comprising an interior volume, at least one inlet, and at least one outlet; providing a mandrel comprising a porous surface that defines an interior volume, the mandrel being disposed within the chamber, the interior volume of the mandrel communicating with the at least one outlet; infusing culture media through the at least one inlet into the interior volume of the chamber; infusing a suspension of cells into the interior volume of the chamber; aspirating culture media from the at least one outlet, wherein culture media passes from the interior volume of the chamber through the pores of the mandrel to the interior volume of the mandrel, and cells are deposited on the surface of the mandrel; removing the mandrel from the chamber after a tissue construct is formed on the mandrel; removing the tissue construct from the mandrel; inserting the tissue construct into the chamber; reversing the flow of culture media within the interior volume of the chamber; and infusing endothelial cells into the interior volume of the chamber, wherein the endothelial cells pass into the tissue construct and are deposited on an inner surface of the tissue construct.