Patent ID: 7906639
Filing Date: 2011-03-15
Classification: C07H,C12Q

Abstract:
1. An oligonucleotide used as a primer consisting of: (a) a 2′-O,4′-C-ethylene nucleotide (ENA) unit which is the third nucleotide from the 3′-end of the oligonucleotide, and the other nucleotides are natural nucleotides, wherein the nucleotide at the 3′-end is defined as the first nucleotide; (b) a nucleotide complementary to the reference nucleotide of a target gene at the 3′-end position thereof; and (c) nucleotides complementary to a nucleotide sequence of the target gene in other positions, or a salt thereof, wherein the oligonucleotide has a base length of 18 to 25 bases.