Patent ID: 8999398
Filing Date: 2015-04-07
Classification: A61K,A61P,C07K,C12N

Abstract:
1. A method for treating a patient infected with a pathogen, the method comprising: a) obtaining a sample of blood mononuclear cells comprising T-cells from a donor; b) activating one or more of the T-cells ex vivo by stimulating the T-cells with soluble anti-CD3 antibody and culture of the T-cells in the presence of T-cell growth factors 10 IU/ml to 500 IU/ml IL-2, or in the presence of 25 to 100 ng/ml IL-7 and 25 to 100 ng/ml IL-15; c) arming the activated T-cells with a heterogeneous population of polyclonal bispecific binding molecules where each bispecific binding molecule comprises a first and a second antigen binding moiety, wherein the first antigen binding moieties specifically bind to an antigen on the surface of a cytotoxic immune cell, said first antigen binding moieties being the same or different, and wherein the second antigen binding moieties are a heterogeneous polyclonal population of antigen binding moieties against multiple different epitopes on one or more pathogenic antigens expressed or presented on the surface of a cell infected with the pathogen, said antigens being the same or different, wherein the first antigen binding moiety is fused to the second antigen binding moiety to form the bispecific binding molecule, and d) administering the armed T-cells to the patient infected with the pathogen.