Patent ID: 6033903
Filing Date: 2000-03-07
Classification: C07K,C12N

Abstract:
A recombinant baculovirus transfer vector, comprising a DNA segment encoding a baculovirus signal peptide joined to a DNA segment encoding an extracellular domain of a hFSH receptor, a fragment thereof, or a variant thereof where at least one amino acid of said extracellular domain is conservatively replaced with a different amino acid, which DNA segment encoding said extracellular domain or fragment or variant thereof being translationally in frame with said signal peptide and operably linked to a baculovirus promoter to form an operable linkage for expressing said extracellular domain or fragment or variant thereof in an insect host cell and secreting at least 0.2 mg/L of said extracellular domain or fragment or variant thereof into a culture medium for said insect host cell, wherein said extracellular domain or fragment or variant thereof binds the hormone ligand of said hFSH receptor.