Patent ID: 7932060
Filing Date: 2011-04-26
Classification: C12Q

Abstract:
1. A method of detecting an analyte in a sample, comprising: (i) combining: (ii) forming a complex comprising the analyte, the first proximity member, the second proximity member, and the hybridization blocker oligonucleotide that is hybridized with the first portion of the first oligonucleotide; (iii) forming a hybrid by displacing the hybridization blocker oligonucleotide from the first portion of the first oligonucleotide of the complex wherein the hybrid comprises the first portion of the first oligonucleotide and the portion of the second oligonucleotide, such that the 3′ terminus of the first oligonucleotide and/ or second oligonucleotide of the hybrid can be extended via a polymerase; (iv) extending via a polymerase the 3′ terminus of the first oligonucleotide and/ or second oligonucleotide and producing an amplicon; (v) amplifying the amplicon and producing an amplification product; and (vi) detecting the amplification product, wherein the detection of the amplification product indicates the presence of the analyte in the sample.