Patent ID: 8445245
Filing Date: 2013-05-21
Classification: A61P,C12N

Abstract:
1. A mutein membrane type serine protease 1 (MT-SP1) protease or biologically active portion thereof, comprising at least one mutation in a scaffold MT-SP1 protease, whereby the substrate specificity or activity of the resulting mutein MT-SP1 polypeptide is altered compared to the scaffold MT-SP1 polypeptide, wherein: the mutein exhibits altered specificity or activity for a VEGFR; the mutation in the scaffold MT-SP1 is selected from among mutations corresponding to D60bI, D60bF, D60bR, D60bA, R60cI, R60cF, R60cD, R60cA, R60cW, F97N, F97D, F97E, F97A, F97W, F97R, F99Y, F99W, F99N, F99D, F99E, F99A, F99V, F99R, M180E, M180Y, M180R, M180A, Q192A, Q192V, Q192D, Q192R, Q192F, W215F, W215Y, W215I, W215D, W215R, D217A, D217V, D217F, D217E and D217R, based on chymotrypsin numbering; and the scaffold MT-SP1 polypeptide has a sequence of amino acids that is at least 95% identical or is identical to the amino acid sequence of a wild type MT-SP1 whose sequence is set forth in SEQ ID NO:1 or SEQ ID NO:2 or is a species variant of the wild type MT-SP1 of SEQ ID NO:1, or is a catalytically active portion thereof.