Patent ID: 7879579
Filing Date: 2011-02-01
Classification: B01J,B82Y,C12Q,C40B

Abstract:
1. A method of forming arrays of oligonucleotides on a solid support comprising: providing a solid support having an array of positions each suitable for attachment of a capture oligonucleotide; attaching linkers to the solid support surface, wherein the linkers are suitable for coupling capture oligonucleotides to the solid support, at each of the array positions; and selecting nucleotide multimers, wherein a selected nucleotide multimer has a nucleotide sequence that differs from the nucleotide sequence of another selected nucleotide multimer by at least 2 nucleotides, wherein no two dimers forming a nucleotide multimer are complementary to each other and the multimers would not result in self-pairing or hairpin formation; and assembling the nucleotide multimers as capture oligonucleotides, wherein the nucleotide multimers are selected so that each of the plurality of capture oligonucleotides, formed from a plurality of assembled nucleotide multimers and attached to the solid support at each array position, have greater than sixteen nucleotides and have nucleotide sequences selected to hybridize with complementary oligonucleotide target sequences under uniform hybridization conditions across the array of oligonucleotides with minimal cross-reactivity and so that each capture oligonucleotide of the array differs in sequence from other adjacent capture oligonucleotides, when aligned to each other by at least 25% of the nucleotides, wherein the nucleotide multimers are formed from multiple nucleotides linked together.