Patent ID: 7771922
Filing Date: 2010-08-10
Classification: G01N

Abstract:
1. A biosensor, comprising: a substrate member; a layer containing a photo-reactive crosslinking agent applied to said substrate member, wherein said crosslinking agent is attached directly to said substrate member; a pattern of active receptive material areas defined on said substrate by attachment with said crosslinking agent, said receptive material comprising molecules specific for an analyte of interest; a pattern of inactive blocking material areas interposed with said substrate by attachment with said crosslinking agent, said inactive blocking material areas being interposed with said active receptive material areas and being non-reactive with the analyte of interest; said active receptive material and inactive blocking material areas defined on said substrate by a masking process wherein a mask is placed over said substrate member prior to irradiating said substrate member with an energy source sufficient for activating said photo-reactive crosslinking agent such that areas exposed by said mask define one of said active receptive material and inactive blocking material areas, and said areas shielded by said mask define the other of said blocking material and receptive material areas; and wherein when said biosensor is exposed to a medium containing said analyte of interest, the analyte binds to said receptive material in said active areas and subsequently facilitate diffraction of transmitted or reflected light in a diffraction pattern corresponding to said active receptive material areas, wherein said diffraction pattern is visible to the naked eye.