Patent ID: 7860694
Filing Date: 2010-12-28
Classification: C12Q,Y10T

Abstract:
1. A method of selecting a pair of probe sequences for detecting a target sequence in a reaction sample comprising the target sequence and a plurality of non-target sequences, the method comprising: selecting an anchoring location in a target sequence, wherein the anchoring location is a location in the target sequence at which a first non-target sequence in a plurality of non-target sequences is different from the target sequence, wherein the first non-target sequence has a sequence similarity to the target sequence that is identical to or higher than the sequence similarity of other non-target sequences in the plurality of non-target sequences; selecting a first probe designing region in the target sequence, wherein the first probe designing region is a fixed region in the target sequence comprising the anchoring location, wherein the first probe designing region is represented by formula 1: wherein, i is the anchoring location and n is the length of a probe; selecting a second probe designing region, wherein the second probe designing region is represented by formula 2 when the length of the probe is an odd number and by formula 3 when the length of the probe is an even number: identifying a location in the second probe designing region of the target sequence at which a second non-target sequence in the plurality of non-target sequences differs from the target sequence; selecting the identified location as a probe selection location; selecting a matched probe sequence, wherein the probe selection location is at the center of the matched probe sequence; and selecting a mismatched probe sequence, wherein the mismatched probe sequence is longer than the matched probe sequence, wherein the probe selection location is at a first location in the mismatched probe sequence and the mismatched location is at a second location in the mismatched probe sequence, wherein the steps are executed by a suitably programmed computer.