Patent ID: 9044530
Filing Date: 2015-06-02
Classification: A61L

Abstract:
1. A process to form a bone grafting material, comprising: providing a porous collagen scaffold; inserting the collagen scaffold into a perfusion chamber of a perfusion flow system; continuously providing a mineralization perfusion fluid flow comprising polyaspartic acid through the collagen scaffold at a flow rate to provide dynamic intrafibrillar mineralization of the collagen scaffold; forming a collagen/hydroxyapatite composite scaffold; contacting the collagen/hydroxyapatite composite scaffold with mesenchymal stromal cells to form a cell seeded scaffold; perfusing a fluid including oxygen and one or more nutrients through the cell seeded scaffold; remodeling the cell seeded scaffold; and forming a bone tissue extracellular matrix on the cell seeded scaffold comprising fibronectin, type I collagen and biglycan so as to form a remodeled scaffold having 95% porosity comprising pore size of about 250 μm.