Patent ID: 8563245
Filing Date: 2013-10-22
Classification: C12N

Abstract:
1. A method of modifying a surface of a substrate with bio-molecules, comprising the steps of: providing a DNA probe, having 18 to 3000 bases of repeated sequences, immobilized on a substrate, wherein each of a plurality of affinity binding tags is attached to the DNA probe via single-strained deoxyribonucleic acid (ssDNA) being complementary to each of the repeated sequences and conjugated to each of the plurality of affinity binding tags, and a plurality of first proteins which respectively conjugated to an affinity tag is attached to the DNA probe through an interaction between the affinity binding tag and the affinity tag in a reversible manner; adding a plurality of second proteins, respectively conjugated with the same affinity tag as that of the first proteins, with a concentration greater than that of the first protein; and replacing the first protein attached to the DNA probe with the second protein through a competition.