Patent ID: 6911305
Filing Date: 2005-06-28
Classification: C09K,C12Q,G01N,Y10S

Abstract:
1. A method of detecting polynucleotide analytes using chemiluminescence comprising the steps of: (a) providing a first film including a complex comprising a polynucleotide analyte bound to a sensitizer or a sensitizer-labeled probe; (b) providing a second film comprising at least one solid chemiluminescent precursor component immobilized therewith that is capable of producing a triggerable chemiluminescent compound; (c) placing said first and second films in sufficient proximity to each other to permit singlet oxygen produced from excitation of the sensitizer on said first film and to react with said chemiluminescent precursor on said second film; (d) exposing said films to suitable conditions to form said triggerable chemiluminescent compound on said second film; (e) allowing said triggerable chemiluminescent compound to be triggered by an activating source to produce a detectable light signal on said second film; and (f) detecting and/or recording said resultant signal on said second film.