Patent ID: 8304234
Filing Date: 2012-11-06
Classification: A61K,A61P,C07K,C12N,Y02A

Abstract:
1. A method of inducing an immune response against an antigen in an individual comprising the step of: injecting into tissue of said individual at a site on said individual's body, a) a polynucleotide function enhancer and b) DNA molecules that comprise DNA sequences that encode said antigen and are operably linked to regulatory sequences which are functional in cells of said individual, wherein said DNA molecules are taken up by cells in said tissue and said antigen is produced by expression of said DNA sequence in said cells; wherein said polynucleotide function enhancer is a compound having a formula selected from the group consisting of: wherein: R1 is C═O; R2 is C1-C10 alkyl including branched alkyls; R3 is hydrogen, amine, C1-C5 alkylamine, C1-C5, C1-C5 dialkylamine; R2 and R3 can form a cyclic alkyl, a C1-C10 alkyl substituted cyclic alkyl, a cyclic aliphatic amine, a C1-C10 alkyl substituted cyclic aliphatic amine, a heterocycle, a C1-C10 alkyl substituted heterocycle including a C1-C10 alkyl N-substituted heterocycle; R4 is Ar, R2 or C1-C5 alkoxy, a cyclic alkyl, a C1-C10 alkyl substituted cyclic alkyl, a cyclic aliphatic amine, a C1-C10 alkyl substituted cyclic aliphatic amine, a heterocycle, a C1-C10 alkyl substituted heterocycle and a C1-C10 alkoxy substituted heterocycle including a C1-C10 alkyl N-substituted heterocycle; R5 is C═NH; R6 is Ar, R2 or C1-C5 alkoxy, a cyclic alkyl, a C1-C10 alkyl substituted cyclic alkyl, a cyclic aliphatic amine, a C1-C10 alkyl substituted cyclic aliphatic amine, a heterocycle, a C1-C10 alkyl substituted heterocycle and a C1-C10 alkoxy substituted heterocycle including a C1-C10 alkyl N-substituted heterocycle; and, R7 is Ar, R2 or C1-C5 alkoxy, a cyclic alkyl, a C1-C10 alkyl substituted cyclic alkyl, a cyclic aliphatic amine, a C1-C10 alkyl substituted cyclic aliphatic amine, a heterocycle, a C1-C10 alkyl substituted heterocycle and a C1-C10 alkoxy substituted heterocycle including a C1-C10 alkyl N-substituted heterocycle.