Patent ID: 7504244
Filing Date: 2009-03-17
Classification: C07K,C12N,C12P

Abstract:
1. A process of constructing a genetically modified strain having compactin (ML-236B) hydroxylation ability comprising transforming a host with a plasmid construct having a promoter DNA sequence, a cytochrome P-450 encoding gene, wherein the cytochrome P-450 encoding gene is obtained from the group consisting of S. lividans, S. carbophilus , and S. helvaticus and a ferredoxin reductase (fdxshe) encoding gene, wherein the fdxshe encoding gene has the nucleotide sequence of SEQ ID NO: 1 or a nucleotide sequence that encodes the amino acid sequence of SEQ ID NO: 2 and wherein the cytochrome P-450 encoding gene encodes a cytochrome P-450 having compactin hydroxylating capability in combination with the protein encoded by the fdxshe encoding gene.