Patent ID: 6355792
Filing Date: 2002-03-12
Classification: C12N

Abstract:
A process for the isolation and purification of nucleic acids from a liquid sample, comprising:(a) providing a liquid sample which contains nucleic acids; (b) providing a carrier comprising an inorganic oxide material containing hydroxyl groups; (c) diluting the sample from step (a) with an acid binding buffer such that the sample is acidified by the binding buffer; (d) treating the sample from (c) with the carrier from (b) so that the nucleic acids become bound to said carrier, with the proviso that the binding buffer is essentially free of ions in concentrations >200 mM, ionic detergents and chaotropic substances; (e) separating the carrier with the bound nucleic acids from the rest of the sample and the binding buffer; and (f) eluting nucleic acids bound to the carrier in (d) with an alkaline solution.