Patent ID: 8715955
Filing Date: 2014-05-06
Classification: G01N,Y10T

Abstract:
1. A method for laser microdissection comprising: providing a first substrate having a surface; applying a layer of biological material to the surface of the first substrate; providing a second substrate having a surface with a transfer film disposed thereon, wherein the transfer film has adhesive characteristics upon activation by electromagnetic energy; identifying at least one targeted portion of biological material to be microdissected, wherein the at least one targeted portion includes desired and undesired biological material; bringing the transfer film into juxtaposition with the first substrate on the side of the biological material in the location of the at least one targeted portion of biological material; activating a first laser source so as to describe at least one closed or substantially closed path around the at least one targeted portion of biological material to be microdissected, wherein the first laser source is configured to erode the biological material along the described path and defining an interior and exterior; activating a second laser source and directing the second laser source at the interior of the at least one described path so as to activate at least one region of the transfer film so that the at least one activated region of transfer film adheres to the at least one interior portion of biological material, wherein the at least one activated region of transfer film adheres to at least one portion of the undesired biological material; and separating the second substrate with its attached transfer film and the at least one adhered targeted portion of biological material from the remaining layer of biological material.