Patent ID: 6979538
Filing Date: 2005-12-27
Classification: A61K,C07K,C12N,C40B

Abstract:
1. A method of obtaining a nucleic acid encoding a proteinaceous binding domain that binds a predetermined target material comprising: (a) expressing a plurality of different chimeric potential binding proteins, each said protein comprising (i) a potential binding domain which is an antibody variable domain and (ii) an outer surface transport signal for obtaining the display of the potential binding domain on the outer surface of a filamentous phase, (b) displaying said chimeric potential binding proteins on the outer surfaces of a population of filamentous phage, said population thereby collectively displaying a plurality of different potential binding domains which differ from each other in amino acid sequence, each phage including a nucleic acid construct comprising a nucleic acid sequence encoding one of said binding domains; (c) contacting said phage with the target material such that said potential binding domains and the target material may interact; (d) separating phage displaying a potential binding domain that binds the target material from phage that do not so bind, and (e) recovering at least one phage displaying on its outer surface a chimeric binding protein comprising a successful binding domain (SBD) which bound said target, said phage enclosing SBD-encoding nucleic acid, and amplifying said SBD-encoding nucleic acid in vivo or in vitro.