Patent ID: 7476520
Filing Date: 2009-01-13
Classification: C12N,C12Q

Abstract:
1. A method for analyzing a beta-lactamase of the SHV family of beta-lactamases in a clinical sample, the method comprising: providing a clinical sample suspected of containing a beta-lactamase nucleic acid of the SHV family of beta-lactamases; providing a pair of oligonucleotide primers, wherein the primers specifically hybridize with a beta-lactamase nucleic acid of the SHV family of beta-lactamases, wherein one primer of the pair is complementary to at least a portion of the sense strand of the beta-lactamase nucleic acid and the other primer of the pair is complementary to at least a portion of the antisense strand of the beta-lactamase nucleic acid, and wherein at least one of the primers is selected from the group consisting of: 5′-ATC GTC CAC CAT CCA CTG CA-3′ (SEQ ID NO:6); 5′-TAG TGG ATC TTT CGC TCC AG-3′ (SEQ ID NO:8); 5′-CAC TCA AGG ATG TAT TGT G-3′ (SEQ ID NO:10); and full-length complements thereof; annealing the primers to the beta-lactamase nucleic acid of the SHV family of the beta-lactamases if the beta-lactamase nucleic acid of the SHV family of beta-lactamases is in the sample; simultaneously extending the annealed primers from a 3′ terminus of each primer of the pair and synthesizing extension products that are complementary to the nucleic acid strands of the beta-lactamase nucleic acid annealed to each primer of the pair wherein the extension products after separation from one strand of the beta-lactamase nucleic acid serve as templates for the synthesis of extension products of other strand of the beta-lactamase nucleic acid; separating the extension products; and analyzing the separated extension products for a region characteristic of the beta-lactamase of the SHV family if the beta-lactamase nucleic acid of the SHV family of beta-lactamases is in the sample.