Patent ID: 7741026
Filing Date: 2010-06-22
Classification: A01K,A61K,A61P,C07K,C12N,G01N,Y10T

Abstract:
1. A method of recovering recombinant protein expressed by open reading frame 2 (ORF′2) of PCVB comprising the steps of: A) cloning said recombinant ORF′2 from PCVB into a transfer vector; B) transferring the portion of said transfer vector containing said recombinant ORF′2 into a virus; C) infecting cells in media with said virus; D) causing said virus to express the protein from said ORF′2 and the cells to secrete said protein into the media; E) separating the cells from the media; and F) recovering said expressed ORF′2 protein from the media.