Patent ID: 9157103
Filing Date: 2015-10-13
Classification: C12N,C12P,C12Y

Abstract:
1. A Y. lipolytica recombinant yeast cell comprising: (a) at least one genetic modification in the YALI0C17545 endogenous gene and at least one genetic modification in the YALI0E28534 endogenous gene; and (b) an exogenous gene encoding a fatty acyl reductase (FAR) protein, wherein the exogenous gene is operably linked to a promoter, wherein the FAR protein has at least 80% amino sequence identity to an amino acid sequence selected from the group consisting of SEQ ID NO: 2, 4, 6, and 65-73, wherein the at least one genetic modification comprises: (i) a deletion of all or a portion of the coding region of the endogenous gene; (ii) a mutation in the endogenous gene such that the gene encodes a polypeptide having reduced activity; (iii) a modified regulatory sequence that reduces expression of the endogenous gene; or (iv) any combination of (i)-(iii).