Patent ID: 7629163
Filing Date: 2009-12-08
Classification: A61P,C07K,C12N,C12P,C12Y

Abstract:
1. A yeast or filamentous fungal host cell that does not have an alpha-1,6 mannosyltransferase activity with respect to an N-glycan on a glycoprotein, comprising a nucleic acid encoding an alpha-1,2 mannosidase enzyme selected to have optimal activity in the endoplasmic reticulum (ER) or Golgi of said host cell, the enzyme comprising: (a) an alpha-1,2 mannosidase catalytic domain having optimal alpha-1,2 mannosidase activity in said ER or Golgi at a pH between 5.1 and 8.0; fused to (b) a cellular targeting signal peptide not normally associated with the catalytic domain and selected to target the mannosidase catalytic domain to said ER or Golgi apparatus; wherein upon passage of recombinant glycoproteins through the ER or Golgi apparatus of said host cell, in excess of 30 mole % of the N-glycan structures thereto have a Man