Patent ID: 7537694
Filing Date: 2009-05-26
Classification: G01N,Y10T

Abstract:
1. A liquid chromatography device comprising: a source of electrolytic solution, an injector for injecting a specimen into an eluting liquid, a first separating column for holding the specimen before and when an electrolytic solution is supplied to the specimen in the first separating column so that components of the specimen are extracted by the electrolytic solution from the specimen in the first separating column, a first pump for feeding the eluting liquid to transfer the specimen from the injector to the first separating column and feeding the electrolytic solution to extract the components of the specimen from the specimen in the first separating column, first and second trap columns each of which is capable of holding the components of the specimen extracted from the specimen in the first separating column, a second pump for feeding another eluting liquid to one of the first and second trap columns after the components of the specimen are held by the one of the first and second trap columns, a second separating column for holding, when at least one of the components of the specimen transferred with the another eluting liquid from the one of the first and second trap columns to the second separating column is eluted by the another eluting liquid from the components thereof in the second separating column, remainder at least one of the components of the specimen, a detector for detecting the at least one of the components of the specimen eluted from the components thereof in the second separating column, and an operation controller for controlling said first and second pumps, wherein: the operation controller is configured to control operations of the first and second pumps so that when said second pump feeds said another eluting liquid from the one of the first and second trap columns to the second separating column before said second pump feeds said another eluting liquid from the other one of the first and second trap columns to the second separating column, the second pump changes a concentration of an organic solvent included by the another eluting liquid from first concentration for extracting the at least one of the components from the components of the specimen in the second separating column to second concentration, and keeps the second concentration lower than the first concentration with the first pump feeding the eluting liquid from the first pump through the other one of the first and second trap columns while preventing the eluting liquid from flowing through the first separating column.