Patent ID: 7445921
Filing Date: 2008-11-04
Classification: C12N,C12P

Abstract:
1. A method for transglycosylation, comprising a reaction of transferring a galactosylβ1→3N-acetylgalactosaminyl group from a sugar donor containing a sugar chain having a galactosylβ1→3N-acetylgalactosaminyl group bound in the configuration of an α anomer, to a sugar chain acceptor, in the presence of a protein having endo-α-N-acetylgalactosaminidase activity and transglycosylation activity, wherein said protein is selected from the group consisting of: (A) a protein having the amino acid sequence of SEQ ID NO:2, and (B) a protein having the amino acid sequence of SEQ ID NO: 2 including a total of one to thirty amino acids mutations in said amino acid sequence of SEQ ID NO: 2, the mutations being one or more selected from the group consisting of substitution, deletion, insertion, and addition, and wherein the protein exhibits endo-α-N-acetylgalactosaminidase activity and transglycosylation activity at a residual activity of at least 80% as compared to the protein having the amino acid sequence of SEQ ID NO: 2 at 50° C. and pH 8.