Patent ID: 9193983
Filing Date: 2015-11-24
Classification: C12Q,G01N

Abstract:
1. A method for characterizing an agent, comprising: treating a culture of eukaryotic cells comprising a DEL selection marker with an agent (“treated portion”) or without an agent (“untreated portion”); measuring the mitochondrial activity of the treated portion of the cell culture in the presence of a suitable selection medium; measuring the mitochondrial activity of the untreated portion of the cell culture in the presence of said selection medium; and quantitating the change in mitochondrial activity and DEL induction between the treated portion and the untreated portions of the cell culture, wherein the change in mitochondrial activity and DEL induction indicates in a simultaneous manner both the cytotoxicity and mutagenicity of the agent; wherein the eukaryotic cells comprise a construct having an auxotrophic gene disrupted by a colorigenic enzyme gene, and the colorigenic enzyme gene is disrupted by a polynucleotide encoding a selectable marker.