Patent ID: 7795026
Filing Date: 2010-09-14
Classification: A61K,C12N

Abstract:
1. A method of obtaining a human embryoid body derived (EBD) cell comprising: (a) culturing primordial germ cells in a media comprising human basic fibroblast growth factor and lacking leukemia inhibitory factor under conditions that allow formation of a solid or cystic embryoid body having a 3-dimensional morphology; (b) disaggregating the solid or cystic embryoid body under enzymatic conditions to provide a constituent cell or embryoid body derived (EBD) cell; and (c) culturing the EBD cell on a defined extracellular matrix, wherein the EBD cell forms disaggregated single cells upon dissociation from embryoid bodies (EB) and proliferates for at least 30 population doublings without being immortal under the conditions of step (a).