Patent ID: 6054291
Filing Date: 2000-04-25
Classification: A61K,C07K,C12N

Abstract:
A method of producing a desired polypeptide comprising culturing a host cell transformed with a plasmid wherein the plasmid comprises:a DNA vector comprising a promoter, a ribosomal binding site, and a unique NdeI site containing an ATG codon, such NdeI site being so positioned downstream of the ribosomal binding site that insertion of DNA encoding a desired polypeptide preceded by the sequence5' TATG 3' - 3' AC 5' into the vector cleaved with NdeI permits transcription of the DNA into mRNA and translation of such mRNA into the polypeptide preceded by methionine; andDNA encoding the polypeptide inserted therein under suitable conditions such that the polypeptide is produced; andrecovering the polypeptide.