Patent ID: 6387268
Filing Date: 2002-05-14
Classification: B01D,B01J,G01N

Abstract:
A method of identifying whether a compound is a specific ligand for at least one cellular receptor comprising (1) contacting an artificial membrane support having non-covalently immobilized thereon, said at least one cellular receptor to a liquid flow system comprising said compound,and (2) determining whether said compound becomes specifically bound to said support wherein said cellular receptor is immobilized such that its conformational structure after immobilization permits specific binding to ligands that are bound by said at least one cellular receptor when it is comprised in its native cellular environment, said artificial membrane support is contained in a liquid flow system; and said artificial membrane support is produced by the following steps: (i) obtaining an immobilized artificial membrane (IAM) liquid chromatographic (LC) stationary phase comprising a phospholipid monolayer; (ii) contacting said IAM LC stationary phase with at least one solubilized receptor or receptor-ligand output under conditions wherein that at least one solubilized receptor or receptor-ligand output becomes non-covalently immobilized in the phospholipid monolayer of said IAM LC stationary phase such that the tertiary structure of said at least one immobilized cellular receptor permits specific binding to ligands that are bound by said at least one cellular receptor when it is comprised in its native cellular environment; and (iii) contacting said artificial membrane support comprising at least one non-covalent immobilized cellular receptor to a liquid flow system.