Patent ID: 6143566
Filing Date: 2000-11-07
Classification: A01K,C12N

Abstract:
A method of selectively performing homologous recombination on a particular nucleotide sequence contained in a recombination deficient host cell comprising:(a) introducing a recombination cassette into the recombination deficient host cell, wherein the recombination cassette contains a nucleic acid that selectively integrates into the particular nucleotide sequence when the host cell is induced to support homologous recombination, and wherein the host cell cannot independently support homologous recombination; and(b) inducing the host cell to transiently support homologous recombination wherein the nucleic acid integrates into the particular nucleotide sequence; and wherein unselected nucleotide sequence rearrangements and deletions which are indicative of host cells that support homologous recombination, are not evident with a restriction endonuclease digestion map analysis with HindIII, EcoRI, XhoI, or AvrII.