Patent ID: 8105820
Filing Date: 2012-01-31
Classification: B82Y,G01N

Abstract:
1. An assay chip for investigation of a functionality of non-lipid molecules and their interactions with molecules, comprising: a nanopore substrate having a plurality of nanopores and alignment marks, said nanopore substrate having a thickness and said nonapores having a diameter in a range of 50 to 2000 nm resulting in an aspect ratio in a range of 0.5 to 2; b) a substantially planar support layer deposited on said nanopore substrate and having a plurality of nanopores corresponding to and aligned with said nanopores of said nanopore substrate; c) a biologically effective layer configured to host at least one of a non-lipid molecule and functional molecule, deposited on said support layer and covering the plurality of nanopores, resulting in accessible nanopores from both sides of the biologically effective layer for measurements, wherein the biologically effective layer is a biomembrane isolated from one of prokaryotic and eukaryotic cells, wherein the biologically effective layer is a lipid bilayer formed by preparation and later fusion of lipid vesicles or is a functional layer of supramolecular assembly, and said biologically effective layer retaining biological functionality.