Patent ID: 7078586
Filing Date: 2006-07-18
Classification: C07K,C12N

Abstract:
1. A DNA construct comprising: a plurality of coupled DNA molecules encoding a papaya ringspot virus coat protein or a fragment thereof, each of which is at least 200 nucleotides in length and at least one of which has a length that is insufficient to impart resistance to papaya ringspot virus to plants transformed with that DNA molecule, wherein the at least one DNA molecule is a fragment of a nucleotide sequence encoding a papaya ringspot virus coat protein having an amino acid sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 16, SEQ ID NO: 18, and SEQ ID NO: 20, said DNA molecules being in a sense or antisense orientation in the DNA construct, and collectively achieving post-transcriptional gene-silencing and imparting resistance to papaya ringspot virus to plants transformed with said DNA construct.