Patent ID: 6352824
Filing Date: 2002-03-05
Classification: C07F,C07K,G01N,Y10S,Y10T

Abstract:
An electrochemically detectable compound of the formula whereinR and R1 are the same or different and are 2,2â€²-bipyridyl, 4,4â€²-disubstituted-2,2â€²-bipyridyl, 5-5â€²-disubstituted,-2,2â€²-bipyridyl, 1,10-phenanthrolinyl, 4,7-disubstituted-1,10-phenanthrolinyl, or 5,6-disubstituted-1,10-phenanthrolinyl, wherein each substituent is a methyl, ethyl, or phenyl group, R5 is 4-substituted-2,2â€²-bipyridyl or 4,4â€²-disubstituted-2,2â€²-bipyridyl wherein the 4-substituent is the group Bâ€”(L)kâ€”Q(CH2)iâ€” and the 4â€²-substituent is a methyl, ethyl or phenyl group; R, R1 and R5 are coordinated to Os through their nitrogen atoms; Q is O, S, or NR4 wherein R4 is hydrogen, methyl or ethyl; â€”Lâ€” is a divalent linker; k is 1 or 0; i is 1, 2, 3, 4, 5 or 6; B is hydrogen or a group comprising a ligand that binds to a specific binding partner; d is +2 or +3; X and Y are anions selected from monovalent anions chloride, bromide, iodide, fluoride, tetrafluoroborate, perchlorate, and nitrate and divalent anions sulfate, carbonate or sulfite wherein x and y are independently 0, 1, 2, or 3 so that the net charge of XxYy is âˆ’2 or âˆ’3.