Patent ID: 6153392
Filing Date: 2000-11-28
Classification: C07K,G01N

Abstract:
An assay for the detection of an antibody against an HBcAg or an independent core-like antigen-adjacent protein of a positive stranded RNA virus in a sample, comprising:(a) providing an HBcAg complexed with a protein selected from the group consisting of a serum albumin and a core-like antigen-adjacent protein of a positive stranded RNA virus, to provide an HBcAg-protein complex, and providing an independent core-like antigen-adjacent protein of a positive stranded RNA virus complexed to a protein, to provide an independent core-like antigen-adjacent protein complex;(b) contacting the HBcAg-protein complex and the independent core-like antigen-adjacent protein complex with the sample under conditions suitable and for a time sufficient for the HBcAg-protein complex and the independent core-like antigen-adjacent protein complex to bind to one or more antibodies against the HBcAg or the independent core-like antigen-adjacent protein present in the sample, thereby providing one or more antibody-bound complexes; and(c) detecting the one or more antibody-bound complexes, and therefrom determining whether the sample contains an antibody against HBcAg or the independent core-like antigen-adjacent protein.