Patent ID: 9181521
Filing Date: 2015-11-10
Classification: C12M,C12N

Abstract:
1. A bioreactor system for a cell culture process comprising: a vessel configured to contain biological or other sensitive substances, the vessel defining an interior upper portion, an interior lower portion and a longitudinal axis; and one or more upward flowing impeller assemblies disposed in the interior lower portion of the vessel and configured to direct fluids containing contain biological or other sensitive substances in the vessel from the interior lower portion of the vessel to the interior upper portion of the vessel in an orientation generally parallel to the longitudinal axis; a gas intake coupled to the vessel and configured to deliver a flow of a sweep gas from one or more external sources of gas to the interior upper portion of the vessel and a gas exhaust coupled to the vessel and configured to remove gases contained in the interior upper portion of the vessel; wherein the upward flowing impeller assembly produces a rolling top surface renewal of the fluids containing contain biological or other sensitive substances in the vessel that promotes surface gas exchange between the fluids and gases in the interior upper portion of the vessel.