Patent ID: 8275557
Filing Date: 2012-09-25
Classification: G16B

Abstract:
1. A computer-implemented method of validating DNA sequencing data from fragment sequence data of one or more DNA fragments, comprising: obtaining from a user a target specification; using said fragment sequence data for said target specification to select from a set of more than one possible reference sequences stored in a database, a selected reference sequence having a highest correlation with said fragment sequence data; aligning automatically the fragment sequence data with the selected reference sequence using a computer; identifying automatically sequence positions where nucleotide codes of the aligned fragment sequence data and the selected reference sequence do not correspond; and using a computer in generating a contig as a consensus sequence from the fragment sequence data aligned with the selected reference sequence, the generating comprising inserting into the consensus sequence, at sequence positions having non-corresponding nucleotide codes in the fragment sequence data, a code indicating ambiguity.