Patent ID: 6287760
Filing Date: 2001-09-11
Classification: C07K,C12N,G01N

Abstract:
A diagnostic tool for the identification of anti-HCMV antibodies in the human serum, said diagnostic tool comprising a means of solid support, a first section of which bears a plurality of viral proteins (vp) which are obtained from the purified virions, with said viral proteins being concentrated in corresponding bands and the bands being supported by said first section of the solid support, spaced one from the other in accordance with a predetermined pattern; with one of said bands being formed by at least one viral protein of approximately 150 kD molecular weight; with said diagnostic tool comprising:(i) Said means of solid support comprising, in addition, a second section containing a plurality of bands spaced among themselves in accordance with a predetermined pattern, with at least one first band of said second section including a single recombinant protein (rp) which comprises a first region reproducing a sequence of amino acids corresponding to at least one epitope of the viral protein pp150, and a second region reproducing at least a portion of the sequence of amino acids of an exogenous protein;(ii) At least one second band of the second section including a recombinant protein which comprises a first region reproducing a sequence of amino acids corresponding to at least one immunogenic epitope of a first non-structural protein of the HCMV virus, and a second region reproducing said at least a portion of the sequence of amino acids of said exogenous protein; and:(iii) At least one control band formed by said at least a portion of the sequence of amino acids of said exogenous protein, said at least a portion of the sequence of amino acids of said exogenous protein in (i) being identical to said at least a portion of the sequence of amino acids of said exogenous protein in (ii) and being further identical to said at least a portion of the sequence of amino acids of said exogenous protein in (iii).