Patent ID: 6570061
Filing Date: 2003-05-27
Classification: A01K,A61K,C07K,C12N

Abstract:
A method of producing a transgenic mouse having a genome comprising a targeted, functional replacement of a mouse immunoglobulin constant region gene segment with a human immunoglobulin constant region gene segment comprising, selecting a transgenic mouse comprising a targeted, functional replacement of the mouse immunoglobulin constant region gene segment with said human immunoglobulin constant region gene segment, wherein said mouse is the progeny of a surrogate mother that contained a mouse blastocyst comprising a mouse embryonic stem cell, wherein said mouse embryonic stem cell was transfected with a recombination vector comprising a human immunoglobulin constant region gene segment, DNA sequences homologous to the mouse immunoglobulin constant region gene segment, a selectable marker gene and recombinase recognition sites flanking said selectable marker gene, wherein said selectable marker gene is removed by a non-mammalian recombinase.