Patent ID: 7008773
Filing Date: 2006-03-07
Classification: A61P,C07K,G01N

Abstract:
1. A method for identifying test substances with antiapoptotic activity, comprising i) culturing cells selected from the group consisting of endothelial cells, smooth muscle cells, and fibroblasts which express both CD47 and the integrin α ii) causing the cells to produce an apoptosis-inducing substance by culturing under static or turbulent flow conditions, and/or adding a substance which simultaneously binds CD47 and α iii) adding a test substance, and iv) determining a change in apoptosis rate by determining the portion of cells with apoptotic nuclei by one of DAPI staining, tunnel assay, DNA ladder, annexin staining, or measuring increased calcium influx into the cells, wherein a decrease in apoptosis rate in step iy) is indicative of the test substance having antiapoptotic activity.