Patent ID: 8138325
Filing Date: 2012-03-20
Classification: C12N,C12P,C12Y,Y02E

Abstract:
1. A method for producing a polypeptide, comprising: (a) cultivating a recombinant fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a first nucleic acid sequence encoding the polypeptide operably linked to a second nucleic acid sequence comprising a promoter variant comprising SEQ ID NO: 4; or a hybrid or a tandem promoter thereof, wherein the hybrid promoter, or the tandem promoter comprises a deletion spanning the nucleotide sequence of CGGGAGAGTGTCAAAT at positions −272 to −257 upstream of the Fusarium venenatum glucoamylase promoter corresponding to nucleotides 1838 to 1853 of SEQ ID NO: 1 and a substitution of ATA with CGG at positions −147 to −145 upstream of the Fusarium venenatum glucoamylase promoter corresponding to nucleotides 1963 to 1965 of SEQ ID NO: 1; and (b) isolating the polypeptide from the cultivation medium.