Patent ID: 7855057
Filing Date: 2010-12-21
Classification: G01N

Abstract:
1. A multiplex detection apparatus for detecting plural different protein isoforms in a sample comprising a mixture of proteins, the multiplex detection apparatus comprising: (a) an apparatus or reagents for digesting said mixture of proteins to produce epitope-bearing peptide fragments, such that each epitope-bearing fragment comprises at least portions of the expression products of exon(s) encoding at least a portion of the respective proteins comprising first and second epitope; (b) a set of immobilized capture agents, individual ones of which bind to either first or second epitopes on peptide fragments generated by digestion of proteins in said sample, wherein said first epitopes are different from said second epitopes; and (c) a set of detectably labeled binding agents which bind to respective second epitopes on respective said epitope-bearing peptide fragments, the binding of a capture agent-detectably labeled binding agent pair being unambiguously indicative of the presence of respective isoforms in the sample.