Patent ID: 9068221
Filing Date: 2015-06-30
Classification: C12Q,G01N

Abstract:
1. A method of detecting an allele of a human identification panel, the method comprising: binding specifically a set of probes to a single stranded nucleic acid segment derived from a locus of the human identification panel, the segment including a microsatellite region having between 5 and 30 k-mer repeat units, wherein k is in a range of 2 to 6, the set of probes including a first subset of k-mer binding probes complementary to the k-mer repeat units; directing the segment through a porin protein nanopore of a nanopore device with an applied voltage, the bound set of probes being stripped from the segment as the segment passes through the porin protein nanopore; measuring with the nanopore device an ion current signal including current spikes corresponding to the stripping of probes of the first subset of k-mer binding probes as the segment passes through the protein nanopore of the nanopore device, a number of current spikes of the ion current signal indicative of the number of k-mer repeat units; detecting the allele based on a characteristic of the current spikes of the ion current signal, wherein the characteristic includes the number of the current spikes.