Patent ID: 6261761
Filing Date: 2001-07-17
Classification: C12Q,G01N

Abstract:
A method of determining the ability of a compound to stimulate cyclic AMP formation comprising:(a) obtaining a cellular sample that has a defect in the amount or activity of NF1 protein;(b) applying said compound to an aliquot of said cellular sample;(c) determining the amount of cyclic AMP produced by said aliquot;(d) adding NF1 protein to another aliquot of said cellular sample;(e) applying said compound to said another aliquot of said cellular sample; and(f) determining the amount of cyclic AMP produced by said another aliquot;wherein an increase in the production of cyclic AMP in step (f) compared with that in step (c) demonstrates the ability of said compound to stimulate cyclic AMP formation.