Patent ID: 8399255
Filing Date: 2013-03-19
Classification: A01H

Abstract:
1. A method for producing ryegrass plants, comprising: a) germinating at least one ryegrass seed on an auxin-containing MS medium to produce a shoot, removing the end of the shoot comprising the first internode, and transferring the removed end to an auxin-containing MS medium to produce callus from the shoot end; b) transferring the callus to a shoot-induction medium comprising an auxin, a cytokine, and CuSO4 until shoots are formed; c) followed by transferring the callus comprising the shoots to a flowering medium comprising an auxin, CuSO4 and at least one substituted phenylurea cytokinin analog; and d) allowing the shoots on the callus to develop at least one flower bud, thus producing a ryegrass plant which is male sterile or partially male sterile.