Patent ID: 8697394
Filing Date: 2014-04-15
Classification: C12N,C12P

Abstract:
1. A process for making a glycoprotein in a Pichia pastoris host cell engineered to produce glycoproteins having GlcNAc 2 Man 3 GlcNAc 2 N-glycans comprising the step of introducing into the cell one or more nucleic acid molecules, each molecule encoding a fusion protein comprising a catalytic domain of an N-acetylglucosaminyltransferase selected from the group consisting of: N-acetylglucosaminyltransferase IV, N-acetylglucosaminyltransferase V, N-acetylglucosaminyltransferase VI, and N-acetylglucosaminyltransferase IX, linked to a heterologous cellular targeting peptide comprising nucleotides 1-108 of the S. cerevisiae MNN2 gene that targets the fusion protein to the Golgi of the host cell, whereby the glycoprotein is produced in the host cell comprising N-glycans that have the structure GlcNAc 3 Man 3 GlcNAc 2 , GlcNAc 4 Man 3 GlcNAc 2 , or GlcNAc 5 Man 3 GlcNAc 2 .