Patent ID: 6544733
Filing Date: 2003-04-08
Classification: C07K,C12N

Abstract:
A process for identifying a nucleic acid sequence encoding a protein conferring resistance against a plant fungal pathogen or elicitor comprising the steps of:(a) selecting a non-host plant resistant to the fungal pathogen or elicitor of interest; (b) amplifying nucleotide sequences corresponding to: (i) class I resistance gene homologs using primers selected from the group consisting of SEQ ID NO:40, SEQ ID NO:41, SEQ ID NO:42, SEQ ID NO:43, SEQ ID NO:44, and SEQ ID NO:45; and (ii) class II resistance gene homologs using primers selected from the group consisting of SEQ ID NO:46, SEQ ID NO:47, and SEQ ID NO:48 from DNA of said non-host plant; (c) identifying an isolated resistance gene homolog from step (b); (d) using the isolated resistance gene homolog from step (c) to recover one or more full-length resistance gene homologs present in said non-host resistant plant; (e) screening said resistance gene homologs for functionality by transforming tissue of a fungal pathogen-susceptible plant with said one or more resistance gene homologs; (f) challenging said transformed tissue with elicitor or fungal pathogen; and (g) observing functional activity against the fungal pathogen of interest.