Patent ID: 7179643
Filing Date: 2007-02-20
Classification: C12M,Y10S

Abstract:
1. A sterilizable bio-reactor system for ex vivo expansion of haemopoietic stem cells derived from Umbilical Cord Blood to provide a portable pilot plant, comprising: at least one pair of reactor culture assemblies, each said reactor culture assembly including at least one vessel and a cover, and each said reactor assembly having a separate inlet port and a separate outlet port; a single case and a carbon dioxide incubator for each said at least one pair of reactor culture assemblies; each said reactor culture assembly being maintained in said single case and kept in said carbon dioxide incubator, thereby enabling other assemblies to be mounted in said carbon dioxide incubator without disturbing the assemblies already in said single case; a scaffolding material positioned on a base of each said reactor culture assembly to provide a micro environment either identical to or substantially identical to the micro environment in human bone marrow whereby self-renewal property of haemopoietic stem cells remain intact and do not lose their self-renewal property; a reservoir and two additive points to said reservoir and an outlet for each said reactor culture assembly; and, fitting the reactor culture assembly in said case, and said case being provided with a gas inlet and then keeping the reactor culture assembly in said carbon dioxide incubator configurated to receive and condition the reactor culture assembly during different stages of the cell growth processes whereby different reactor culture assembly can be fitted within different cases so that more than one reactor culture assemblies can be mounted in a carbon dioxide incubator without disturbing the other reactor assemblies.