Patent ID: 7858322
Filing Date: 2010-12-28
Classification: A61K,C07K,C12N,G01N

Abstract:
1. A method for determining whether a test compound inhibits binding between a TRPM7 protein and a PDZ domain-containing polypeptide, comprising: (i) contacting a TRPM7 protein or a fragment thereof containing at least the four C-terminal amino acids of the protein with a PDZ domain-containing polypeptide in the presence and absence of the test compound; and (ii) measuring the amount of complex formed between the TRPM7 protein or fragment thereof and the PDZ domain-containing polypeptide in the presence and absence of the test compound, wherein less complex is formed in the presence relative to the absence of the test compound indicating the test compound is an inhibitor of binding between the TRPM7 protein and the PDZ domain-containing polypeptide.