Patent ID: 9085628
Filing Date: 2015-07-21
Classification: A61K,A61P,C07K

Abstract:
1. An isolated binding member for human GM-CSFRα, wherein the binding member comprises an antibody molecule that inhibits binding of GM-CSF to GM-CSFRα and wherein the binding member binds at least one residue of the sequence Tyr-Leu-Asp-Phe-Gln at positions 226 to 230 of human GM-CSFRα as shown in SEQ ID NO: 206, and wherein the binding member binds to human GM-CSFRα extra-cellular domain with an affinity (KD) of less than 4 nM in a surface plasmon resonance assay, said antibody molecule comprising a heavy chain variable region and a light chain variable region, comprising one of: (a) a heavy chain complementarity determining region (H-CDR)1, a H-CDR2, and a H-CDR3 of the heavy chain variable region set forth as SEQ ID NO: 2, and a light chain complementarity determining region (L-CDR)1, a L-CDR2, and a L-CDR3 of the light chain variable region set forth as SEQ ID NO: 208; (b) a H-CDR1, a H-CDR2, and a H-CDR3 of the heavy chain variable region sequence set forth as SEQ ID NO: 12, and a L-CDR1, a L-CDR2, and a L-CDR3 of the light chain variable region sequence set forth as SEQ ID NO: 210; (c) a H-CDR1, a H-CDR2, and a H-CDR3 of the heavy chain variable region sequence set forth as SEQ ID NO: 22, and a L-CDR1, a L-CDR2, and a L-CDR3 of the light chain variable region sequence set forth as SEQ ID NO: 210; (d) a H-CDR1, a H-CDR2, and a H-CDR3 of the heavy chain variable region sequence set forth as SEQ ID NO: 32, and a L-CDR1, a L-CDR2, and a L-CDR3 of the light chain variable region sequence set forth as SEQ ID NO: 210; (e) a H-CDR1, a H-CDR2, and a H-CDR3 of the heavy chain variable region sequence set forth as SEQ ID NO: 42, and a L-CDR1, a L-CDR2, and a L-CDR3 of the light chain variable region sequence set forth as SEQ ID NO: 210; (f) a H-CDR1, a H-CDR2, and a H-CDR3 of the heavy chain variable region sequence set forth as SEQ ID NO: 52 or 248, and a L-CDR1, a L-CDR2, and a L-CDR3 of the light chain variable region sequence set forth as SEQ ID NO: 218 or 250; (g) a H-CDR1, a H-CDR2, and a H-CDR3 of the heavy chain variable region sequence set forth as SEQ ID NO: 62, and a L-CDR1, a L-CDR2, and a L-CDR3 of the light chain variable region sequence set forth as SEQ ID NO: 210; (h) a H-CDR1, a H-CDR2, and a H-CDR3 of the heavy chain variable region sequence set forth as SEQ ID NO: 72, and a L-CDR1, a L-CDR2, and a L-CDR3 of the light chain variable region sequence set forth as SEQ ID NO: 210; (i) a H-CDR1, a H-CDR2, and a H-CDR3 of the heavy chain variable region sequence set forth as SEQ ID NO: 82, and a L-CDR1, a L-CDR2, and a L-CDR3 of the light chain variable region sequence set forth as SEQ ID NO: 210; (j) a H-CDR1, a H-CDR2, and a H-CDR3 of the heavy chain variable region sequence set forth as SEQ ID NO: 92, and a L-CDR1, a L-CDR2, and a L-CDR3 of the light chain variable region sequence set forth as SEQ ID NO: 210; (k) a H-CDR1, a H-CDR2, and a H-CDR3 of the heavy chain variable region sequence set forth as SEQ ID NO: 102, and a L-CDR1, a L-CDR2, and a L-CDR3 of the light chain variable region sequence set forth as SEQ ID NO: 228; (l) a H-CDR1, a H-CDR2, and a H-CDR3 of the heavy chain variable region sequence set forth as SEQ ID NO: 22, and a L-CDR1, a L-CDR2, and a L-CDR3 of the light chain variable region sequence set forth as SEQ ID NO: 230; (m) a H-CDR1, a H-CDR2, and a H-CDR3 of the heavy chain variable region sequence set forth as SEQ ID NO: 22, and a L-CDR1, a L-CDR2, and a L-CDR3 of the light chain variable region sequence set forth as SEQ ID NO: 232; (n) a H-CDR1, a H-CDR2, and a H-CDR3 of the heavy chain variable region sequence set forth as SEQ ID NO: 132, and a L-CDR1, a L-CDR2, and a L-CDR3 of the light chain variable region sequence set forth as SEQ ID NO: 234; (o) a H-CDR1, a H-CDR2, and a H-CDR3 of the heavy chain variable region sequence set forth as SEQ ID NO: 142, and a L-CDR1, a L-CDR2, and a L-CDR3 of the light chain variable region sequence set forth as SEQ ID NO: 236; (p) a H-CDR1, a H-CDR2, and a H-CDR3 of the heavy chain variable region sequence set forth as SEQ ID NO: 152, and a L-CDR1, a L-CDR2, and a L-CDR3 of the light chain variable region sequence set forth as SEQ ID NO: 238; (q) a H-CDR1, a H-CDR2, and a H-CDR3 of the heavy chain variable region sequence set forth as SEQ ID NO: 152, and a L-CDR1, a L-CDR2, and a L-CDR3 of the light chain variable region sequence set forth as SEQ ID NO: 240; (r) a H-CDR1, a H-CDR2, and a H-CDR3 of the heavy chain variable region sequence set forth as SEQ ID NO: 172, and a L-CDR1, a L-CDR2, and a L-CDR3 of the light chain variable region sequence set forth as SEQ ID NO: 242; (s) a H-CDR1, a H-CDR2, and a H-CDR3 of the heavy chain variable region sequence set forth as SEQ ID NO: 182, and a L-CDR1, a L-CDR2, and a L-CDR3 of the light chain variable region sequence set forth as SEQ ID NO: 244; or (t) a H-CDR1, a H-CDR2, and a H-CDR3 of the heavy chain variable region sequence set forth as SEQ ID NO: 192, and a L-CDR1, a L-CDR2, and a L-CDR3 of the light chain variable sequence region set forth as SEQ ID NO: 246.