Patent ID: 7935506
Filing Date: 2011-05-03
Classification: C07K,C12P

Abstract:
1. A method for producing a lower alkyl ester of a -L-aspartyl-L-phenylalanine, comprising: (i) cultivating a recombinant (ii) synthesizing the lower alkyl ester of α-L-aspartyl-L-phenylalanine from an aspartic acid or N-acyl-L-aspartic anhydride and the L-phenylalanine obtained from the medium, wherein L-phenylalanine production is enhanced by increasing activity of a protein in a cell of the recombinant (A) a protein which consists of the amino acid sequence of SEQ ID NO:2; (B) a protein which consists of the amino acid sequence of SEQ ID NO:2 except wherein one to five amino acids are deleted, substituted, inserted, or added; or (C) a protein which consists of the amino acid sequence that is encoded by a nucleotide sequence that hybridizes with the complement of the nucleotide sequence of SEQ ID NO: 1 under stringent conditions comprising 60° C., 1×SSC, 0.1% SDS; wherein said protein in (A), (B), or (C) makes said bacterium resistant to L-phenylalanine, fluoro-phenylalanine or 5-fluoro-DL-tryptophan and wherein the activity of said protein in (A), (B), or (C) is increased by: a) transformation of said bacterium with a DNA encoding said protein and expressing the protein in said bacterium; b) replacing the native promoter that precedes the DNA encoding said protein on the chromosome of the bacterium with a more potent promoter; or c) introduction of multiple copies of the DNA encoding said protein into the chromosome of said bacterium and expressing the protein in said bacterium.