Patent ID: 6168935
Filing Date: 2001-01-02
Classification: C12P

Abstract:
A method of manufacturing an optically active alcohol substituted with one or more halogen atoms, comprising: a step of producing the optically active alcohol substituted with one or more halogen atoms by asymmetric reduction of a halogen-substituted ketone with a stereo-selective secondary alcohol dehydrogenase together with an alcohol for regeneration of reduced nicotinamide adenine dinucleotide, wherein the reaction of asymmetric reduction is performed at a temperature of 30.degree. C. or less, wherein said stereo-selective secondary alcohol dehydrogenase is an enzyme having the following physicochemical properties: (a) effects: oxidizes alcohols with oxidized nicotinamide adenine dinucleotide as coenzyme to form ketones or aldehydes, and reduces ketones or aldehydes with reduced nicotinamide adenine dinucleotide as coenzyme to form alcohols; and (b) substrate specificity: acts on aliphatic alcohols comprising aromatic substituents as substrates in an oxidative reaction, has a greater effect on secondary alcohols than on primary alcohols, oxidizes the S-form of 2-butanol or the S-form of 1-phenylethanol, and acts on aldehydes or aliphatic ketones comprising aromatic substituents as substrates in a reductive reaction; and wherein said stereo-selective secondary alcohol dehydrogenase has a molecular weight of approximately 40,000 when measured by SDS-PAGE if in a form that oxidizes the S-form of 2-butanol, or has a molecular weight of approximately 51,000 when measured by SDS-PAGE or approximately 107,000 when measured by gel filtration if in a form that oxidizes the S-form of 1-phenylethanol.