Patent ID: 6794144
Filing Date: 2004-09-21
Classification: A61K,A61P,C07K,C12N

Abstract:
A method for generating proteins containing artificial SH3 domains having ligand binding affinity that is higher than the affinity of corresponding wild-type SH3 domain which comprises:a) producing a collection of DNA fragments encoding SH3 domains containing randomized mutations in a variable domain of an RT-loop (RRT-SH3 domains) that corresponds to amino acids 69-74 of Hck, b) generating recombinant libraries comprising said variable RT-loon domains, c) subjecting said libraries to affinity or functional selection steps to identify non-naturally occurring RT-loop domains, and d) selecting proteins containing domains with a binding affinity that is higher than the binding affinity of the corresponding wild-type SH3 domain.