Patent ID: 7262030
Filing Date: 2007-08-28
Classification: C12Q

Abstract:
1. A method of producing hybrid DNA with a single strand overhang that includes a target site, comprising the steps of: obtaining a first primer which hybridizes to a 5′ strand of deoxyribonucleic acid (DNA) containing said target site, a second primer which hybridizes to a complementary strand of DNA 3′ of said target site, and a third primer which hybridizes to said complementary strand of DNA 5′ of said target site; producing by nested polymerase chain reaction (PCR) using said first primer, said second primer, and said third primer, an outer amplicon which includes said target site and an inner amplicon which excludes said target site, wherein said first and second primers generate said outer amplicon and said first and third primers generate said inner amplicon; and forming at least one of