Patent ID: 8227198
Filing Date: 2012-07-24
Classification: G01N

Abstract:
1. A method of determining whether or not associations between a given protein and other proteins in a cell are specific, the method comprising: (a) providing a first sample of the cells, wherein neither the given protein nor the other proteins are tagged, (b) providing a second sample of the same cells in which the given protein contains a tag, wherein the given protein and the other proteins are metabolically labeled, (c) mixing and lysing the first cell sample and the second cell sample to provide a mixture of proteins, (d) binding the tag of the given protein to a substrate, (e) isolating proteins associated with the tagged given protein bound to the substrate, whereby the associated proteins comprise: (f) determining whether each associated protein is labeled or a mixture of labeled and unlabeled proteins, wherein if the associated protein is labeled, then that protein was specifically associated in the cell with the tagged given protein.