Patent ID: 6613884
Filing Date: 2003-09-02
Classification: C07K

Abstract:
A method for separating human serum albumin from a mixture including a human serum albumin analogue, said method comprising:a) contacting a mixture containing human serum albumin and a human serum albumin analogue with a ligand wherein said ligand: i) is derived from a fragment of streptococcal protein G comprising the complete A3 albumin binding domain, said fragment further lacks IgG-binding ability or &agr;2-macroglobulin-binding ability, or both; and ii) is directly or indirectly attached to a base matrix insoluble in aqueous media; or iii) is attached to a base matrix insoluble in aqueous media after formation of a human serum albumin-ligand complex; b) binding said human serum albumin to said ligand to form a complex; and c) separating said human serum albumin from the mixture comprising said human serum albumin analogue.