Patent ID: 7132233
Filing Date: 2006-11-07
Classification: C07K,C12N,C12Q

Abstract:
1. A method for detecting the presence of West Nile virus (WNV) in a biological sample, the method comprising: isolating nucleic acids from a biological sample suspected of containing WNV; amplifying the nucleic acids using a sense and an antisense primer wherein each of the primers is not more than about 60 nucleotides in length and is sufficiently complementary to a portion of the antisense and sense strands, respectively, of the isolated nucleic acid to hybridize therewith to allow amplification of said WNV nucleic acids, and (a) the sense primer comprises SEQ ID NO:34; (b) the antisense primer comprises SEQ ID NO:35; and detecting the presence of the amplified WNV nucleic acids as an indication of the presence of WNV in the sample.