Patent ID: 6979828
Filing Date: 2005-12-27
Classification: C12M,C12Q

Abstract:
1. A method for immediately determining microorganisms, comprising the steps of: double-staining a sample of a selected microorganisms with appropriate fluorescent reagents, said sample comprising a plurality microorganism bodies selecting a micro-tube having a light permeability, wherein an internal diameter of the micro-tube is chosen to correspond to a diameter of the selected microorganism such that the microorganism bodies must pass through in single file at a particular location along the length thereof; passing the sample through the micro-tube, illuminating an excited light having a central wavelength of 488 nm through said micro-tube, whereby-the microorganism bodies emit flourescent light at a known wavelength, detecting the fluorescence of the microorganism bodies at said particular location by photodiodes or a multiplier photo-tube; converting the image of the detected fluorescence into electric signals; and determining from said electrical signals one or more of the amount of the microorganism bodies, a type of the microorganisms, and whether the microorganism bodies are vital or dead.