Patent ID: 8008002
Filing Date: 2011-08-30
Classification: C12Q

Abstract:
1. A method for determining a target nucleic acid sequence, the method comprising the steps of: (a) contacting a preparation comprising a target nucleic acid sequence and a non-target nucleic acid sequence, the target nucleic acid sequence and the non-target nucleic acid sequence each having a first region of common sequence upstream of a first region of dissimilar sequence upstream of a second region of dissimilar sequence—with an oligonucleotide primer complementary to at least a portion of the first region of common sequence, under conditions to hybridize the primer thereto; (b) contacting the resulting preparation with a first labeled nucleotide bearing a first label, wherein the first labeled nucleotide is complementary to a first template nucleotide comprised in the first region of dissimilar sequence of either the target nucleic acid sequence or the non-target nucleic acid sequence, under conditions to incorporate the first labeled nucleotide either into the primer hybridized to the target nucleic acid sequence or into the primer hybridized to the non-target nucleic acid sequence but not into both; (c) subjecting the so treated preparation to a sequencing reaction, thereby extending the primer to form one or more first-labeled sequencing products comprising the first labeled nucleotide and one or more non-first-labeled sequencing products comprising no first labeled nucleotide; and (d) determining the target nucleic acid sequence by determining at least a portion of the sequence of the first-labeled sequencing products and/or the non-first-labeled sequencing products, thereby determining at least the second region of dissimilar sequence of the target nucleic acid sequence.