Patent ID: 6309837
Filing Date: 2001-10-30
Classification: C12Q,Y10S,Y10T

Abstract:
A method for objectively identifying a genotype resistant to Fusarium wilt disease in a whole cucurbit plant, plant extract, or plant seed by using cycles of a polymerase chain reaction, said method comprising the steps of:(a) removing a sample of plant tissue from a cucurbit plant to be tested for a Fusarium-resistant genotype;(b) extracting genomic DNA from said sample for use as a DNA template;(c) replicating a desired region in the DNA template by binding a specific oligonucleotide primer set selected from the primer sets comprising Seq ID: 1 and Seq ID:2; and Seq. ID:3 and Seq ID:4 to said DNA template in a complimentary fashion, said primer set designed to give rise to a desired PCR product having a size different from the size of a product produced from amplifying undesired genomic DNA;(d) amplifying complimentary DNA by conducting the polymerase chain reaction from about 30 cycles to about 40 cycles to produce a PCR product; and(e) examining said PCR product to determine if said product is amplified from a Fusarium-resistant cucurbit genotype.