Patent ID: 8185320
Filing Date: 2012-05-22
Classification: G01N,G02B,G06K,G06T

Abstract:
1. A computer implemented method for localizing and quantitating a particular biomarker present in individual cells of interest contained in a tissue sample comprising: a) incubating the tissue sample with a stain that specifically labels the biomarker; b) obtaining a high resolution image of the stain wherein the image comprises 1024×1024 pixel locations having an intensity value for each pixel in the tissue sample using an upright or inverted optical microscope so as to obtain: c) subtracting the second image from the first image to obtain a third image, wherein for each pixel location a percentage of the out-of-focus image pixel intensity is subtracted from the corresponding in-focus image pixel intensity to obtain the third image; and d) analyzing the third image to obtain a stain intensity and location of the stain so as to thereby localize and quantitate the biomarker.