Patent ID: 6339065
Filing Date: 2002-01-15
Classification: A61K,A61P,C07K,C12N

Abstract:
A method of expressing a foreign gene in a mammalian cell comprising the steps of:transfecting the mammalian cell with a replication-competent, transformation-negative vector comprising (a) at least one papovavirus origin of replication, (b) a first DNA sequence encoding a mutant form of papovavirus large T antigen which (1) contains a replication-competent binding site for said origin of replication and which (2) is negative for binding to wild-type p53 and to retinoblastoma tumor suppressor gene product due to a mutation in a codon in the p53 binding domain of said large T antigen and a mutation in a codon in the RB binding domain of said large T antigen, said first DNA sequence being operatively linked to a first promoter which is functional in said mammalian cell, and a second DNA sequence encoding the foreign gene operatively linked to a second promoter which is functional in the mammalian cell, whereby the transfected cell subsequently express the foreign gene, where the foreign gene is expressed in vivo.