Patent ID: 6346386
Filing Date: 2002-02-12
Classification: C12Q,Y10T

Abstract:
A method for determining whether a DNA sequence contains an alteration comprising:obtaining a DNA segment of interest, wherein the DNA segment of interest comprises one or more copies of the DNA sequence; attaching a GC-rich clamp to the DNA segment of interest; fluorescently labeling the DNA segment of interest; mixing the DNA segment of interest with a denaturant to create a mixture; heating the mixture of the fluorescently labeled DNA segment of interest and denaturant to melt the DNA segment of interest; monitoring fluorescence to obtain a melting temperature of the DNA segment of interest; and comparing the melting temperature of the DNA segment of interest with a melting temperature of a wild-type sequence, wherein a melting temperature for the DNA segment of interest that is different from the melting temperature for the wild-type sequence indicates an alteration in the DNA sequence.