Patent ID: 9029086
Filing Date: 2015-05-12
Classification: C12Q

Abstract:
1. A method for detecting an analyte in a sample, wherein the analyte can simultaneously bind four analyte-binding domains, comprising: (a) contacting said sample with at least one set comprising a cassette oligonucleotide and first, second, third and fourth proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain, wherein the respective analyte-binding domains simultaneously bind to the analyte; wherein the nucleic acid domains of said first and said third proximity probes include respective first overlapping regions which are complementary and can hybridize and the nucleic acid domains of said second and said fourth proximity probes include respective second overlapping regions which are complementary and can hybridize; and wherein said cassette oligonucleotide and the nucleic acid domain of said third proximity probe include respective third overlapping regions which are complementary and can hybridize, and said cassette oligonucleotide and the nucleic acid domain of said fourth proximity probe include respective fourth overlapping regions which are complementary and can hybridize; (b) hybridising the respective overlapping regions of said proximity probes and said cassette oligonucleotide; and (c) detecting the analyte in the sample based on detecting a hybridization product of step (b).