Patent ID: 8067241
Filing Date: 2011-11-29
Classification: G01N

Abstract:
1. A method of antigen retrieval of a formaldehyde-fixed tissue sample comprising the steps of: incubating the formaldehyde-fixed tissue sample in a first antigen retrieval solution at a temperature of greater than 90° C.; transferring the formaldehyde-fixed tissue sample to a second antigen retrieval solution; incubating the formaldehyde-fixed tissue sample in the second antigen retrieval solution at a temperature of greater than 90° C.; and wherein the first antigen retrieval solution comprises a buffer solution having a pH range of between about 5 and about 7 and the second antigen retrieval solution comprises a buffer solution having a pH range of between about 7.5 and about 11; or the first antigen retrieval solution comprises a buffer solution having a pH range of between about 7.5 and about 11 and the second antigen retrieval solution comprises a buffer solution having a pH range of between about 5 and about 7.