Patent ID: 8828680
Filing Date: 2014-09-09
Classification: G01N

Abstract:
1. A method for high throughput concurrent microorganism identification and antimicrobial susceptibility testing comprising: a) inoculating one or more clear plastic panels having a plurality of wells with a sample to be tested for microorganism identification and antimicrobial susceptibility, wherein the one or more panels comprise a plastic microtiter plate having an opaque top surface and clear plastic wells for determining a microorganism's identification and a microorganism's susceptibility to at least one antimicrobial agent; wherein the plurality of wells comprises at least one growth well, at least one minimum-inhibitory concentration (MIC) antimicrobial test well, and at least one control well; wherein for microorganism identification, the at least one growth well contains an assay reagent, which when acted upon by a microorganism, is indicative of a microorganism family, genus and/or species; wherein for antimicrobial susceptibility testing, the at least one MIC antimicrobial test well contains an antimicrobial agent; b) placing the inoculated one or more clear plastic panels into an apparatus that maintains the inoculated one or more clear plastic panels at a predetermined temperature for a predetermined time; c) incubating the inoculated one or more clear plastic panels in the apparatus at the predetermined temperature for a first predetermined time; d) at a second predetermined time, performing a bichromatic collection process comprising: e) determining the identity of the microorganism from the growth well turbidity reading and the growth well fluorescent reading; f) determining the susceptibility of the microorganism to the antimicrobial agent in each of the at least one MIC antimicrobial test well by performing the following steps for each respective MIC antimicrobial test well: