Patent ID: 6020313
Filing Date: 2000-02-01
Classification: A61K,C07K,Y10S

Abstract:
Method for inducing bone formation in a mammal comprising administering an amount of an extract of Saos-2 cells sufficient to promote bone healing or bone formation to a mammal in need of said bone healing or bone formation, said extract of Saos-2 cells obtained by contacting a sample of devitalized, freeze dried Saos-2 cells with a denaturing agent in an amount and concentration to permit refolding of any denatured protein to active form or insufficient to denature protein to form a supernatant and a precipitate, applying said supernatant to a protein absorbing gel, and removing protein fractions from said absorbing gel having a molecular weight range of from about 10 kd to about 60 kd, to form an extract of Saos-2 cells.