Patent ID: 8530633
Filing Date: 2013-09-10
Classification: C07K,C12Q,G01N

Abstract:
1. An isolated nucleic acid comprising a polynucleotide sequence which encodes: a glutamate-aspartate receptor (YbeJ) comprising the amino acid sequence of SEQ, ID NO: 28, wherein the YbeJ specifically binds a glutamate or aspartate, and at least one of a donor fluorophore protein moiety fused to the YbeJ or an acceptor fluorophore protein moiety fused to the YbeJ, wherein the coding region of the donor fluorophore protein moiety is inserted between amino acids 58 and 59 of SEQ, ID NO: 28, and the coding region of the acceptor fluorophore protein moiety is inserted at an internal site of the coding region of said YbeJ.