Patent ID: 6255337
Filing Date: 2001-07-03
Classification: A23L,A61K,C11B

Abstract:
A first-pass effective composition of matter, which is the first-pass effective inhibitor in a primary first-pass effective fraction obtained by a process comprising:1. providing an oil of a first-pass citrus,2. removing volatile materials in said oil by subjecting said oil to evaporation, distillation or reduced pressure to provide a residue,3. extracting said residue with acetonitrile and evaporating said acetonitrile to provide an acetonitrile-soluble semi-purified residue,4. washing said semi-purified residue with an ethanol-water mixture optionally containing base to provide a washed semi-purified residue,5. extracting said washed semi-purified residue with acetonitrile to provide an acetonitrile solution,6. washing said acetonitrile solution with an alkane solvent and evaporating acetonitrile from said acetonitrile solution to provide a purified residue, and7. subjecting said purified residue to reverse phase HPLC to provide plural fractions at least one of which is the primary first-pass effective fraction.