Patent ID: 7960157
Filing Date: 2011-06-14
Classification: C12N

Abstract:
1. A blend of two or more DNA polymerases, comprising at least one fusion polypeptide DNA polymerase and at least one non-fusion polypeptide DNA polymerase, wherein said fusion polypeptide DNA polymerase has a proofreading activity and consists of a first polypeptide covalently linked to a second polypeptide, wherein said first polypeptide is a DNA polymerase selected from archaeal DNA polymerases and eubacterial DNA polymerases, and the second polypeptide is a DNA binding polypeptide, wherein the first and second polypeptides are not fused to each other in nature; and wherein said non-fusion polypeptide is a DNA polymerase selected from archaeal DNA polymerases and eubacterial DNA polymerases, said non-fusion polypeptide having a proofreading activity.