Patent ID: 7427483
Filing Date: 2008-09-23
Classification: C12Q,Y02A

Abstract:
1. A procedure using specific biotin labeled nucleotide probes directed to the highly conserved 42-kDa C-terminal region of the Plasmodium falciparum merozoite surface protein 1 (MSP1) gene as a target for the quantitative determination of Plasmodium falciparum DNA, comprising: (a) a procedure for amplifying the 42-kDa C-terminal region of the MSP1 gene from negative control (non-infected) and (b) a procedure for the synthesis of three specific biotin labeled nucleotide probes 7, 8, and 9 directed respectively to three different fragments of 420 bp (fragment 1), 420 bp (fragment 2), and 379 bp (fragment 3) located in the 42-kDa C-terminal region of the MSP1 gene of (c) using the PCR products produced in (a) and probes 7, 8, and 9 synthesized in (b) in an enzyme linked immunosorbent assay (ELISA) to quantitatively determine the 42-kDa C-terminal region of the MSP1 gene in said samples, and thereby quantitatively determining