Patent ID: 9074251
Filing Date: 2015-07-07
Classification: C12N,C12Q

Abstract:
1. A method of sequencing a target nucleic acid, comprising: (a) contacting a target nucleic acid with a plurality of transposons under conditions such that a plurality of the transposons are inserted into the target nucleic acid, wherein the transposons that are inserted into the target nucleic acid each comprise a first transposase recognition site, a second transposase recognition site, and a barcode pair, wherein the barcode pair comprises a first barcode sequence, a second barcode sequence and a linker comprising a fragmentation site disposed between the first and second barcode sequences, wherein the first barcode sequence comprises a first strand and a second strand that is not complementary to the first strand of the first barcode sequence, wherein the barcode pair is disposed between the first and second transposase recognition sites; (b) fragmenting the target nucleic acid at the fragmentation sites thereby preparing a library of template nucleic acid fragments; (c) obtaining sequence reads from the nucleic acid fragments; and (d) assembling a representation of at least a portion of the target nucleic acid from the sequence reads, wherein the assembling comprises determining the order of the sequence reads from the identities of the first barcode sequence and the second barcode sequence of each barcode pair.