Patent ID: 7659073
Filing Date: 2010-02-09
Classification: G01N

Abstract:
1. A diagnostic method for determining the presence of prostate cancer in a subject comprising the steps of: (a) contacting a PSA containing sample from the subject with an antibody or antigen binding fragment thereof selective for [−2]pPSA, wherein the PSA containing sample is selected from the group consisting of prostate tissue, prostate cells from prostate tissue, blood, serum, plasma, seminal fluid, and urine; (b) determining the amount of [−2]pPSA contained in the PSA containing sample; (c) correlating the amount of the [−2]pPSA contained in the PSA containing sample to the presence of prostate cancer in the subject, wherein step (c) further comprises the steps of: wherein a value of the ratio comprising the amount of [−2]pPSA over the amount of free PSA of the subject greater than a cut-off value of the ratio comprising the amount of [−2]pPSA over the amount of free PSA is an indication of prostate cancer in the subject.