Patent ID: 6231871
Filing Date: 2001-05-15
Classification: A61K,A61P,Y02A,Y10S

Abstract:
A method for immunizing an avian species against infection by a pathogenic Salmonella microorganism, said method comprising administering in ovo to an air sac of an egg an attenuated Salmonella microorganism which:(a) exhibits auxotrophy to one or more growth factors selected from the group consisting of phenylalanine, tyrosine, tryptophan and para-aminobenzoic acid such that it is incapable of growing on a minimal medium in the absence of said one or more growth factors; and(b) is capable of colonizing one or more tissues in an embryo prior to hatching in an amount and under conditions effective to induce an immune response in the embryo before or immediately after hatching against a virulent form of said attenuated Salmonella microorganism or a microorganism expressing an immunologically cross-reactive antigen.