Patent ID: 6414122
Filing Date: 2002-07-02
Classification: C07C,G01N,Y10S

Abstract:
A method of preparing a bioconjugate comprising:providing a bifunctional boronic compound complexing conjugate having a first biologically active species, the bifunctional boronic compound complexing conjugate having a formula of General Formula II: wherein group R2 is selected from one of H and OH moieties; wherein group R3 is selected from one of an alkyl and a methylene bearing an electronegative substituent; wherein group Z is a spacer selected from one of (CH2)n and CH2O(CH2CH2O)n2, and n is an integer of from 1 to 5, and n2 is an integer of from 1 to 4; wherein each of group Z2 and Z3 is a spacer selected from one of CH2, CH2CONHCH2, CH2CONH(CH2)n3CONHCH2, and (CH2)n4NHCO(CH2)n5CONHCH2 and n3 is an integer of from 1 to 5, n4 is an integer selected from one of 2 and 3, and n5 is an integer of from 1 to 4; wherein BAS represents a biologically active species; providing at least one boronic compound conjugate having a second biologically active species; conjugating the bifunctional boronic compound complexing conjugate and the at least one boronic compound conjugate.