Patent ID: 6828436
Filing Date: 2004-12-07
Classification: B01D,C12N

Abstract:
A method for purifying an oligonucleotide comprising:(a) loading said oligonucleotide onto the stationary phase of an anion-exchange column; and (b) displacing said oligonucleotide from said stationary phase by an anionic displacer having a molecular weight of less than about 10,000, said anionic displacer having a higher affinity for said stationary phase than said oligonucleotide; wherein said anionic displacer is selected by a method comprising: (i) providing an anion exchange column comprising a stationary phase; said stationary phase comprising counter ions; (ii) contacting said oligonucleotide with said stationary phase; (iii) determining the affinity for said oligonucleotide for said stationary phase; (iv) contacting a displacer candidate with said stationary phase; (v) determining the affinity for said displacer candidate for said stationary phase; (vi) comparing the affinity of said displacer candidate with the affinity of said oligonucleotide; and (vii) selecting said displacer candidate for use as said anionic displacer in the purification of said oligonucleotide provided that said displacer candidate has a higher affinity than said oligonucleotide.