Patent ID: 9097706
Filing Date: 2015-08-04
Classification: G01N

Abstract:
1. A method of determining the blood-brain barrier (BBB) penetration of a chemical compound in an insect, said method comprising the steps: (i) optionally anesthetizing the insect, (ii) fixing the head of the insect, (iii) dissecting out the dorsal part of the insect head so as to expose the brain comprising an intact BBB, eyes, antennas and nerve associations, (iv) treating the brain comprising the intact BBB while in its cuticle shell ex vivo by placing in a solution of a chemical compound, (v) removing the brain from its cuticle shell, (vi) washing and homogenizing or ultra sound disintegrating the brain, (vii) determining the concentration of the chemical compound in the homogenized brain material; and (viii) determining the penetration of the chemical compound through the blood-brain barrier.