Patent ID: 8907060
Filing Date: 2014-12-09
Classification: A61K,A61P,C07K

Abstract:
1. An isolated Pseudomonas exotoxin A (“PE”) comprising a PE amino acid sequence, wherein one or more of amino acid residues N314, D324, Q332, D403, R412, R427, E431, R432, R458, D461, R467, R505, R538, E548, R576, K590, and L597 as defined by reference to SEQ ID NO: 1 are, independently, substituted, provided that when amino acid residue Q332 is substituted, amino acid residue Q332 is substituted with a anine, glycine, or serine; when amino acid residue K590 is substituted, amino acid residue K590 is substituted with alanine, glycine, or serine; when amino acid residue L597 is substituted, amino acid residue L597 is substituted with glycine, serine or glutamine; and when one or more of amino acid residues N314, D324, D403, R412, R427, E431, R432, R458, D461, R467, R505, R538, E548, and R576 are substituted, the one or more of amino acid residues N314, D324, D403, R412, R427, E431, R432, R458, D461, R467, R505, R538, E548, and R576 are, independently, substituted with alanine, glycine, serine or glutamine; wherein the PE optionally has a further substitution of amino acid residue R490, as defined by reference to SEQ ID NO: 1, with alanine, valine, glycine, leucine, isoleucine or glutamine, and wherein the PE optionally has a further substitution of, independently, one or more of amino acid residues E282, E285, P290, R313, P319, E327, E331, R513, E522, and R551, as defined by reference to SEQ ID NO: 1, with alanine, glycine, serine or glutamine.