Patent ID: 8912510
Filing Date: 2014-12-16
Classification: G01N

Abstract:
1. A fluorescence observation apparatus comprising: a laser scanning fluorescence observation unit including a plurality of lasers, a scanning unit that scans a sample by laser light from the plurality of lasers, and a detector that detects fluorescence from the sample excited by the laser light; a wide field fluorescence observation unit including a light source to planar-illuminate the sample, a plurality of excitation filters that are selectively arranged on an optical path of light from the light source and that respectively correspond to the plurality of lasers, and a two-dimensional detector that detects a fluorescence from the sample, wherein the sample is excited by light that has passed through an excitation filter arranged on the optical path of light from the light source; and a dichroic mirror that combines an optical path of the laser light with the optical path of light from the light source; wherein: each of the plurality of excitation filters has an optical property in which a center wavelength of a transmissive band is approximately the same as an oscillation wavelength of a corresponding laser and a bandwidth of a transmissive band is approximately the same as a tolerance width of an oscillation wavelength of a corresponding laser; the laser scanning fluorescence observation unit further includes a plurality of first barrier filters selectively arranged between the dichroic mirror and the detector, each of the first barrier filters corresponding to a respective one of the plurality of lasers; the wide field fluorescence observation unit further includes a plurality of second barrier filters selectively arranged between the dichroic mirror and the two-dimensional detector, each of the second barrier filters corresponding to a respective one of the plurality of lasers; the first barrier filter and the second barrier filter which correspond to a same laser have a same optical property; and when fluorescence observation is performed by using both the laser scanning fluorescence observation unit and the wide field fluorescence observation unit, (i) an excitation filter from the plurality of excitation filters, which corresponds to another laser selected from the plurality of lasers, excluding a laser to be used for fluorescence observation, is arranged on the optical path of light from the light source, (ii) a first barrier filter from the plurality of first barrier filters, which corresponds to the laser to be used for fluorescence observation, is arranged between the dichroic mirror and the detector, and (iii) a second barrier filter from the plurality of second barrier filters, which corresponds to the another laser, is arranged between the dichroic mirror and the two-dimensional detector.