Patent ID: 6027879
Filing Date: 2000-02-22
Classification: C12Q

Abstract:
A method for detecting a target sequence in a sample of nucleic acids comprising:hybridizing the target sequence with a hybridization probe, the hybridization probe including a nucleic acid sequence capable of hybridizing to the target sequence, a plurality of detectable markers, and a plurality of first complexing agents which are distinct from the detectable markers and which are capable of forming a binding pair with a second complexing agent;isolating the sample of nucleic acids including any hybridization probe hybridized to the target sequence from any nonhybridized hybridization probe;immobilizing the hybridized hybridization probe and the target sequence hybridized thereto by binding the first complexing agent attached to the hybridized hybridization probe to a second complexing agent attached to the solid support;isolating the immobilized target sequence from any nonimmobilized nucleic acids; anddetecting the immobilized target sequence by detecting the detectable marker on the hybridized hybridization probe.