Patent ID: 8609413
Filing Date: 2013-12-17
Classification: A61P,C12N

Abstract:
1. A method of preparing one or more neuronal cells in vitro, comprising: a) exposing substantially or essentially undifferentiated mammalian pluripotent or neural stem cells to an effective amount of a differentiation agent under conditions sufficient to enhance differentiation of the stem cells to neural precursor cells as compared to differentiation under similar conditions without the differentiation agent; and b) incubating the neural precursor cells in the absence of the differentiation agent under conditions sufficient to differentiate the neural precursor cells into neurons, astrocytes or oligodendrocytes; wherein the differentiation agent is a pyridine derivative having the following structure: or pharmaceutically acceptable salts, hydrates, tautomers, acetals, ketals, hemiacetals, hemiketals, or optical isomers thereof .