Patent ID: 8232079
Filing Date: 2012-07-31
Classification: C12P

Abstract:
1. An oligosaccharide synthesizer comprising: a container containing a buffer solution; a sample injector for injecting a mixture of a sugar nucleotide solution and a glycosyltransferase into a flow path connected to a reaction tank, said reaction tank having a primer immobilized therein and said reaction tank being used for reaction between said mixture injected out of said sample injector and said primer; a pump for feeding said buffer solution to said sample injector; a plurality of sugar nucleotide containers for storing a plurality of said sugar nucleotide solutions; a plurality of glycosyltransferase containers for storing a plurality of said glycosyltransferases; a mixing tank for mixing a sugar nucleotide solution supplied from one of said sugar nucleotide containers and a glycosyltransferase supplied from one of said glycosyltransferase containers, wherein said mixing tank provides said mixture to said sample injector; and a controller, the controller configured to cause said plurality of sugar nucleotide solutions and said plurality of glycosyltransferase solutions to be supplied to said mixing tank so as to consecutively repeat the reaction in said reaction tank for respective ones of said plurality of sugar nucleotide solutions and said plurality of glycosyltransferase solutions, thereby producing an oligosaccharide.