Patent ID: 9096861
Filing Date: 2015-08-04
Classification: C12N

Abstract:
1. A fertile transplastomic plant comprising one or more plastids engineered to contain one or more expression cassettes for the plastid-encoded expression of genes encoding enzymes for the production of polyhydroxyalkanoate (PHA), wherein the one or more expression cassettes comprise untranslated regions (UTRs) with a sequence length of about 55 nucleotides or fewer, or wherein the one or more expression cassettes comprise a total plastidial DNA content of about 3% or less, wherein the genes encoding enzymes for the production of PHA have codon usage similar to the plastome of the host plant and are inserted into an existing transcriptional unit of the host plastid, and are under the control of the transcriptional unit, and wherein the fertile transplastomic plant produces greater than 10% polyhydroxyalkanoate per unit dry cell weight (dwt) in leaves and produces seeds that are able to germinate and produce viable transplastomic plants.