Patent ID: 8846366
Filing Date: 2014-09-30
Classification: C12N,C12P

Abstract:
1. A synthetic R-hydroxynitrile lyase comprising an amino acid sequence which is a variant of SEQ ID NO: 29, wherein the variant differs from SEQ ID NO: 29 solely by (i) amino acid substitution of alanine with glycine at a position corresponding to position 111 of SEQ ID NO: 29 and (ii) amino acid substitution of one or more positions corresponding to positions selected from 3, 108, 110, 304, 85, 209, 278, 432, 424, 225, 1, and 317 of SEQ ID NO: 29, and wherein if position 110 is substituted then the amino acid substitution is substitution of asparagine with serine, if position 225 is substituted then the amino acid substitution is substitution of asparagine with serine, if position 1 is substituted then the amino acid substitution is substitution of leucine with glutamine, and if position 317 is substituted then the amino acid substitution is substitution of valine with alanine.