Patent ID: 8298819
Filing Date: 2012-10-30
Classification: C12N

Abstract:
1. A cosmid vector having a full length of 15 kb or less characterized in that: 1) it contains an origin of replication (oriV) of an IncP plasmid but does not contain any origin of replication of other plasmid groups; 2) it contains the trfA1 gene of an IncP plasmid; 3) it contains an origin of conjugative transfer (oriT) of an IncP plasmid; 4) it contains the incC1 gene of an IncP plasmid; 5) it contains a cos site of lambda phage and the cos site is located outside the T-DNA; 6) it contains a drug resistance gene expressed in 7) it contains a T-DNA right border sequence of a bacterium of the genus 8) it contains a T-DNA left border sequence of a bacterium of the genus 9) it contains a selectable marker gene for plant transformation located between 7) and 8) and expressed in a plant; and 10) it contains restriction endonuclease recognition site(s) located between 7) and 8) for cloning a foreign gene, wherein the cosmid vector is selected from the group consisting of: the cosmid vector pLC40 consisting of the nucleotide sequence of SEQ ID NO: 2; the cosmid vector pLC40GWH consisting of the nucleotide sequence of SEQ ID NO: 3; the cosmid vector pLC40 bar consisting of the nucleotide sequence of SEQ ID NO: 4; the cosmid vector pLC40GWB consisting of the nucleotide sequence of SEQ ID NO: 5; the cosmid vector pLC40GWHKorB consisting of the nucleotide sequence of SEQ ID NO: 65; the cosmid vector pLCleo consisting of the nucleotide sequence of SEQ ID NO: 66; and the cosmid vector pLC40GWHvG1 consisting of the nucleotide sequence of SEQ ID NO: 7.