Patent ID: 6117641
Filing Date: 2000-09-12
Classification: A61K,C07K,C12Q,G01N

Abstract:
An assay for identifying a compound having potential anti-fungal activity, comprising:(a) forming a reaction mixture including:(i) a fungal geranylgeranyl transferase I (GGPTase I),(ii) a GGPTase substrate, and(iii) a test compound;under conditions wherein, in the absence of the test compound, the GGPTase I and the GGPTase substrate interact; and(b) detecting interaction of the GGPTase substrate with the GGPTase I, wherein a statistically significant decrease in the interaction of the GGPTase substrate and GGPTase I in the presence of the test compound, relative to the level of interaction in the absence of the test compound, indicates a potential anti-fungal activity for the test compound.