Patent ID: 8945831
Filing Date: 2015-02-03
Classification: C12Q,G01N,Y10T

Abstract:
1. A method of genotyping a dog to detect the presence of an allelic variant in the 5′UTR of Cox2 comprising: (a) providing a sample containing DNA from a dog; (b) contacting DNA from the sample with a PCR primers comprising SEQ ID NO:13 and SEQ ID NO:14; (c) detecting the allelic variant of (i) a deletion of 6 nucleotides starting at position −68/−73 and a deletion of 11 nucleotides starting at position −37 of the canine Cox2 gene; (ii) an insertion of 12 nucleotides at position −78 of the canine Cox2 gene, wherein the insertion comprises the nucleotide sequence CGCCTCCGCCTC (SEQ ID NO:31); and/or (iii) an insertion of 24 nucleotides at position −78 of the canine Cox2 gene, wherein the insertion comprises the nucleotide sequence CGCCTCCGCCTCCGCCTCCGCCGC (SEQ ID NO:32); wherein detecting the allelic variant comprises amplifying the nucleotide sequence using the PCR primers; and (d) identifying the dog as having the allelic variant in the 5′UTR of Cox2.