Patent ID: 8691530
Filing Date: 2014-04-08
Classification: C07K,C12N,C12P

Abstract:
1. A process for obtaining aspart insulin comprising the steps of: i) transforming a ii) selecting from the strains transformed in step i), those MUT S recombinant strains containing more than 5 copies of the DNA construct integrated into the yeast genome; iii) selecting from the recombinant strains from step ii) above, the strain having the highest number of copies and having the highest expression level of the aspart insulin precursor iv) fermenting said recombinant strain; v) separating the aspart insulin precursor from the culture medium by chromatography, vi) enzymatically digesting said precursor under conditions suitable for converting at least 70% of aspart insulin precursor into mature aspart insulin; and vii) purifying the aspart insulin obtained from step (vi) by conventional chromatography.