Patent ID: 6451307
Filing Date: 2002-09-17
Classification: A61K,A61P,C12N

Abstract:
A process for isolating a poly(A)-specific 3â€²-exonuclease comprising the following:(a) preparing a crude extract from animal or human cells; (b) precipitating protein present in the crude extract to obtain a precipitate; (c) subjecting the precipitate to chromatography on a weakly basic anion exchanger to obtain a first active fraction; (d) subjecting the first active fraction to affinity chromatography on heparin to obtain a second active fraction; (e) subjecting the second active fraction to chromatography on a strongly basic anion exchanger to obtain a third active fraction; (f) subjecting the third active fraction to affinity chromatography on a dye to obtain a fourth active fraction; (g) subjecting the fourth active fraction to poly(A)-affinity chromatography to obtain a fifth active fraction; and (h) subjecting the fifth active fraction to chromatography on a basic anion exchanger; wherein a poly(A)-specific 3â€²-exonuclease is isolated.