Patent ID: 7659063
Filing Date: 2010-02-09
Classification: B01J,C12Q,C40B,G16B

Abstract:
1. A method of detecting at least one nucleic acid target, comprising contacting a cell sample which may comprise said target(s) with a nuclease protection fragment(s) specific for and which binds to said target(s), exposing the sample to a nuclease effective to digest single stranded nucleic acid, and contacting the resultant sample with a combination which comprises, before the addition of said sample, under conditions effective for said nuclease protection fragment(s) to bind to said combination, wherein said nucleic acid target is lysed from said cells by exposure of said cells in said sample to a lysis solution comprising amounts effective for lysis of about 8 to about 60% v/v of formamide, about 0.01 to about 0.5% w/v of SDS and 0.5-6X SSC buffer.