Patent ID: 6555714
Filing Date: 2003-04-29
Classification: A01N,C12N,C12Q,C12Y,G01N

Abstract:
A method for inhibiting p-hydroxyphenylpyruvate dioxygenase of a plant which comprises:identifying an inhibitor of p-hydroxyphenylpyruvate dioxygenase of the plant by an assay for identifying inhibitors of anabolic p-hydroxyphenylpyruvate dioxygenase from plants, said assay comprising the steps of: incubating an isolated p-hydroxyphenylpyruvate dioxygenase from the plant with a potential inhibitor and, after a suitable substrate reaction time, determining the enzymatic activity of the p-hydroxyphenylpyruvate dioxygenase from the plant incubated with the potential inhibitor in comparison with the enzymatic activity of p-hydroxyphenylpyruvate dioxygenase from the plant which has not been so incubated with the potential inhibitor, whereby a decrease in enzymatic activity of the p-hydroxyphenylpyruvate dioxygenase from the plant incubated with the potential inhibitor identifies that the potential inhibitor is an inhibitor of the plant p-hydroxyphenylpyruvate dioxygenase; and applying to the plant the identified inhibitor of the p-hydroxyphenylpyruvate dioxygenase of the plant.