Patent ID: 6645733
Filing Date: 2003-11-11
Classification: G01N,Y10S

Abstract:
A method for assaying binding between at least one target and at least one probe, said method comprising:providing at least one target comprising a peptide sequence or a peptide analog sequence; providing at least one probe consisting essentially of amino acids or amino acid analogs, and at least one fluorophore; providing a test medium comprising said at least one target and said at least one probe; binding said at least one target to said at least one probe in said test medium to form a binding complex; irradiating said test medium with radiation effective to cause said at least one fluorophore to emit fluorescent light; and detecting a quenching effect on fluorescence emitted by said at least one fluorophore resulting from said binding without measuring polarization of said fluorescence, wherein said binding is specific and sufficiently sensitive to detect bound target at a concentration less than 1.5Ã—10âˆ’11 M, and wherein said method is conducted without separating complexes of said at least one target and said at least one probe from free target and free probe prior to said quenching effect detecting, and without providing a signal quenching agent to quench said emitted fluorescence.