Patent ID: 8329401
Filing Date: 2012-12-11
Classification: C07F,C07H,G01N

Abstract:
1. A method of detecting nucleic acids of interest in a biological sample comprising the steps of: (a) labeling DNA isolated from the biological sample with a first member of a specific binding pair; (b) contacting the DNA of step (a) to a microarray of nucleic acid probes immobilized on a solid support; (c) incubating the microarray of step (b) with a label enzyme conjugated to a second member of the specific binding pair, the label enzyme selected from the group consisting of alkaline phosphatase, acid phosphatase, alpha- and beta-galactosidases, alpha- and beta-glucosidases, esterases, and beta-lactamases; (d) incubating the microarray with a redox inactive reductive species and soluble metal ions, the redox inactive reductive species being a substrate for the label enzyme so that the label enzyme converts the redox inactive reductive species to a redox active reductive species that reduces the soluble metal ions to form a metal precipitate on the microarray; and (e) detecting DNA hybridized to the microarray by detecting the metal precipitate formed on the microarray.