Patent ID: 9023597
Filing Date: 2015-05-05
Classification: C12Q

Abstract:
1. A method for detecting a nucleic acid molecule in a sample, said method comprising i) amplifying a nucleic acid molecule, in a sample, by use of two primers in a polymerase chain reaction (PCR) in the presence of a single stranded nucleic acid probe that is complementary to said nucleic acid molecule, wherein an end of said probe is immobilized via attachment to the apex of a conical dendron that is attached to a solid surface by the base of said conical dendron, ii) completing the last cycle of the PCR to produce amplified nucleic acid molecule, iii) denaturing said amplified nucleic acid material in the presence of said PCR reaction mixture to form a denatured mixture in the presence of the immobilized probe and attached conical dendron, iv) allowing said amplified nucleic acid material, after denaturation, within the same PCR reaction mixture, to hybridize to said probe to form a double stranded complex in the presence of said denatured mixture, and v) detecting said double stranded complex attached to said solid surface.