Patent ID: 7851158
Filing Date: 2010-12-14
Classification: C12Q

Abstract:
1. A method for the enrichment of a nucleic acid sequence, said method comprising: forming a heteroduplex comprising a first nucleic acid sequence and a second nucleic acid sequence, wherein said heteroduplex comprises at least one mismatched base pair between the first nucleic acid sequence and the second nucleic acid sequence; circularizing the heteroduplex to form a circularized heteroduplex (CHET); preferentially cutting the CHET to form a duplex using an enzyme that recognizes the mismatched base pair; ligating a binding moiety nucleic acid sequence (BMNAS) into the duplex to form a binding moiety duplex, wherein said BMNAS comprises: two nucleic acid sequences hybridized to one another and a binding moiety; and selecting the binding moiety by using a purifying moiety to bind to the binding moiety, thereby enriching the second nucleic acid sequence.