Patent ID: 8440403
Filing Date: 2013-05-14
Classification: B01L,G01N,Y10S

Abstract:
1. A method for analyzing a DNA molecule, the method comprising: providing a first reservoir and a second reservoir separated by a substrate, wherein the substrate includes a nanopore, the nanopore includes at least one metallic nanoparticle in an area facing the second reservoir, and the at least one metallic nanoparticle is capable of producing plasmon resonance; inducing a fluorescently labeled single-stranded DNA molecule into the first reservoir; inducing the fluorescently labeled single-stranded DNA molecule to move from the first reservoir to the second reservoir via the nanopore and in close proximity to the at least one metallic nanoparticle; inducing particle plasmon resonance in the at least one metallic nanoparticle in the area facing the second reservoir, the at least one metallic nanoparticle producing, by the particle plasmon resonance, a localized electromagnetic field at the area of the nanopore facing the second reservoir; detecting fluorescence produced by the interaction of (i) the localized electromagnetic field produced by the at least one metallic nanoparticle at the area of the nanopore facing the second reservoir and (ii) the fluorescently labeled single-stranded DNA molecule moving into the second reservoir via the nanopore; and analyzing the fluorescently labeled single-stranded DNA molecule based on the detected fluorescence.