Patent ID: 6326205
Filing Date: 2001-12-04
Classification: A61K,A61P,C07K,C12N

Abstract:
A method for genetically modifying a population of human hematopoietic stem cells, comprising the steps of:culturing ex vivo an initial hematopoietic cell population comprising human CD34.sup.+ Thy-1.sup.+ hematopoietic stem cells in a medium comprising a myeloproliferative receptor (mpl) ligand, wherein said population of hematopoietic stem cells proliferates to expand the number of CD34.sup.+ cells in a final cell population; andtransducing said hematopoietic cell population with a viral vector comprising a gene of interest, wherein said final cell population comprises human hematopoietic stem cells that have been genetically modified by integration of said gene of interest into the cells.