Patent ID: 8980543
Filing Date: 2015-03-17
Classification: C12Q,G01N,G01R,Y02P

Abstract:
1. A method of measuring enzyme activity by a substrate probe based on at least one of two-dimensional and three-dimensional nuclear magnetic resonance method, comprising measuring a change of at least one of two-dimensional and three-dimensional nuclear magnetic resonance signal, wherein the change is caused by recognition of the substrate probe by an active-state enzyme and a chemical reaction proceeding thereafter, wherein the probe has an enzyme recognition site, which is selectively recognized by the active-state enzyme, as at least one constitutional unit, and wherein a group, to which at least three nuclear magnetic resonance active nuclei each having a nuclear spin and a different resonance frequency are connected, is present specifically in the enzyme recognition site, wherein the substrate probe is glucose, and wherein all carbons of 1 to 6 positions of the glucose are labeled with