Patent ID: 8323890
Filing Date: 2012-12-04
Classification: C12Q

Abstract:
1. A method for detecting cytosine methylation and methylated CpG islands within a genomic sample of DNA comprising: contacting a genomic sample of DNA with a modifying agent that modifies unmethylated cytosine to produce a converted nucleic acid; amplifying the converted nucleic acid by means of oligonucleotide primers in the presence of a methylated CpG-specific oligonucleotide probe and a non-methylated CpG-specific probe, wherein one or a plurality of oligonucleotide primers and the CpG-specific probes are capable of distinguishing between unmethylated and methylated nucleic acid; and detecting, in real time during the amplification, the methylated nucleic acid based on amplification-mediated displacement of the probes, wherein a method for detecting cytosine methylation and/or methylated CpG islands within a genomic sample of DNA is afforded.