Patent ID: 6641998
Filing Date: 2003-11-04
Classification: C12N

Abstract:
A method for producing a vector comprising a desired nucleic acid insert from a sample comprising a plurality of vectors, the method comprising:generating first and second extension products from first and second primers annealed to a vector in the sample, wherein the vector comprises a desired nucleic acid insert, wherein the first and second primers anneal to complementary strands of the vector comprising the desired nucleic acid insert, wherein one or both of the first or second primers comprise a nucleic acid sequence found in the desired nucleic acid insert or its complement, wherein the first and second extension products are capable of annealing to each other at both of their terminal ends, and wherein the nucleic acids of the first and second extension products together comprise a sequence for replication in a host and the desired nucleic acid insert; optionally repeating the generating first and second extension products; and combining and annealing the first and second extension products to form a partially double-stranded, replication-competent vector.