Patent ID: 6582927
Filing Date: 2003-06-24
Classification: C12Q

Abstract:
A method for identifying a proteinâ€”protein interaction between a first protein and a second protein comprising the steps of:(a) expressing in a cell which is incapable of activating a Ras protein; (aa) a first nucleic acid sequence encoding a first fusion protein, said first fusion protein comprising a Ras protein mutated such that it cannot localize at the cell membrane and does not require an exchange factor fused to said first protein; and (ab) a second nucleic acid sequence encoding a second fusion protein said second fusion protein comprising said second protein fused to a plasma membrane localization domain; and (b) determining whether there is a phenotypic expression of a functional Ras protein in said cells the presence of a functional Ras protein in said cell indicating a proteinâ€”protein interaction between said first protein and said second protein.