Patent ID: 6632613
Filing Date: 2003-10-14
Classification: C07K,C12Q,G01N,Y10S

Abstract:
A process for performing a fluorescence polarization immunoassay, said process comprising:providing a monoclonal antibody having an analyte-binding site which selectively binds an analyte; providing an oligopeptide tracer comprising an oligopeptide, said oligopeptide having an amino acid sequence which does not form internal disulfide bridges, and which is constructed to selectively bind to the analyte-binding site, and having an N-terminal end, a linker segment of from one to five amino acid residues covalently linked to the N-terminal end of the oligopeptide, and a fluorescent moiety attached to the linker segment contacting the monoclonal antibody with the oligopeptide tracer in a solution and determining a first polarization value of the fluorescent molecule; providing a sample comprising an unknown quantity of the analyte; adding the sample to the solution containing the monoclonal antibody and the oligopeptide tracer and determining a second polarization value of the fluorescent molecule; and comparing the first and second polarization values to make an estimate of the amount of the analyte in the sample.