Patent ID: 7879573
Filing Date: 2011-02-01
Classification: G01N

Abstract:
1. A method for isolating an analyte from a mixture, the method comprising: providing a mixture comprising an iminobiotin marker-conjugated analyte; separating the iminobiotin marker-conjugated analyte from the mixture using a separation matrix; labeling the iminobiotin marker-conjugated analyte in the separation matrix with a detectable biotin-binding molecule, wherein the labeling occurs under conditions sufficient for the biotin-binding molecule to bind to iminobiotin with high affinity; isolating the iminobiotin marker-conjugated analyte from the separation matrix; and removing the detectable biotin-binding molecule from the iminobiotin marker-conjugated analyte under conditions that decrease the binding affinity of the biotin-binding molecule for iminobiotin.