Patent ID: 9040491
Filing Date: 2015-05-26
Classification: C12N

Abstract:
1. A composition comprising a nucleic acid cargo sequence and an isolated snRNA, wherein said cargo sequence is linked to said snRNA, further wherein said snRNA consists of: (a) an Sm site, wherein said Sm site is selected from the group consisting of SEQ ID NO. 1, SEQ ID NO. 2, and SEQ ID NO. 3; (b) a stem-loop structure positioned 3′ to said Sm site, wherein the number of nucleotides between the Sm site and the 3′ stem loop is between 1 and 5 nucleotides, further wherein said stem-loop structure comprises from about 7 to about 12 base pairs in the stem and from about 4 to about 17 nucleotides in the loop; and (c) nucleotides located 3′ from the stem-loop structure consisting of 5-10 nucleotides.