Patent ID: 6280934
Filing Date: 2001-08-28
Classification: C12Q

Abstract:
A transformed yeast cell responsive to G-protein coupled activation of a yeast G.alpha. Gpa2 protein comprising a nucleic acid construct comprising a promoter selected from the group consisting of YMR323w, YBL100c, YDR187c, YEL045c, YBLO96c, YNL028w, YFR056c, PHO4, YCL046w, YPL281c, YJL045w, YGL046w, GLK1, YML058c-a, YJR114w, IDH2, YML035c-a, HSP12, HSP26, YNL134c and YML128c, wherein said promoter is operably linked to a heterologous first DNA sequence which is operably linked to a second DNA sequence, wherein said heterologous first DNA sequence encodes a mammalian G-protein coupled receptor, and wherein said second DNA sequence encodes a yeast G.alpha. Gpa2 protein, such that expression of the first and second DNA sequences produces a fusion protein wherein the yeast G.alpha. Gpa2 protein is linked to the mammalian G-protein coupled receptor, and wherein binding of a ligand to the mammalian G-protein coupled receptor results in alteration of cellular levels or activity of adenylate cyclase and/or cAMP.