Patent ID: 8246947
Filing Date: 2012-08-21
Classification: A61K,A61L,A61P,C12N

Abstract:
1. A method of augmenting autologous fat transfer to a subject, comprising: removing a first portion of adipose tissue that comprises a cell population comprising adipose-derived stem cells from said subject; introducing the removed adipose tissue into a self-contained cell processing unit configured to maintain a closed pathway; isolating said cell population that comprises adipose-derived stem cells from mature adipocytes and connective tissue present in the removed adipose tissue so as to obtain a substantially isolated cell population comprising disaggregated adipose-derived stem cells, while maintaining a closed pathway; concentrating said substantially isolated cell population comprising disaggregated adipose-derived stem cells so as to obtain a concentrated cell population that comprises disaggregated adipose-derived stem cells, while maintaining a closed pathway; mixing said concentrated cell population comprising adipose-derived stem cells with a second portion of unprocessed adipose tissue comprising intact, non-disaggregated tissue fragments obtained from said subject, so as to obtain an adipose tissue composition comprising a mixture of said concentrated cell population comprising disaggregated adipose-derived stem cells and said unprocessed adipose tissue comprising intact, non-disaggregated tissue fragments tissue; and reintroducing said adipose tissue composition into said subject.