Patent ID: 7919462
Filing Date: 2011-04-05
Classification: A61P,C04B,C07K

Abstract:
1. An isolated polypeptide that inhibits plasmin comprising a Kunitz domain having the formula: Met-His-Ser-Phe-Cys-Ala-Phe-Lys-Ala-Xaa10-Xaa11-Gly-Xaa13-Cys-Xaa15-Xaa16-Xaa17-Xaa18-Xaa19-Arg-Trp-Xaa22-Xaa23-Asn-Ile-Phe-Thr-Arg-Gln-Cys-Xaa31-Xaa32-Phe-Xaa34-Xaa35-Gly-Gly-Cys-Xaa39-Xaa40-Asn-Gln-Xaa43-Arg-Xaa45-Glu-Ser-Leu-Glu-Glu-Cys-Lys-Lys-Met-Cys-Thr-Arg-Asp (SEQ ID NO:150), wherein Xaa10 is selected from the group consisting of Asp, Glu, and Tyr; Xaa11 is selected from the group consisting of Thr, Ala, Ser, Val, and Asp; Xaa13 is selected from the group consisting of Pro, Leu, and Ala; Xaa15 is selected from the group consisting of Arg and Lys; Xaa16 is selected from the group consisting of Ala and Gly; Xaa17 is selected from the group consisting of Arg, Lys, and Ser; Xaa18 is selected from the group consisting of Phe and Ile; Xaa19 is selected from the group consisting of Glu, Asp, Pro, Gly, Ser, and Ile; Xaa22 is selected from the group consisting of Tyr and Phe; Xaa23 is selected from the group consisting of Tyr and Phe; Xaa31 is selected from the group consisting of Asp, Glu, Thr, Val, Gln, and Ala; Xaa32 is selected from the group consisting of Thr, Ala, Glu, Pro, and Gln; Xaa34 is selected from the group consisting of Val, Ile, Thr, Leu, Phe, Tyr, His, Asp, Ala, and Ser; Xaa35 is selected from the group consisting of Tyr and Trp; Xaa39 is selected from the group consisting of Glu, Gly, Asp, Arg, Ala, Gln, Leu, Lys, and Met; Xaa40 is selected from the group consisting of Gly and Ala; Xaa43 is selected from the group consisting of Asn and Gly; and Xaa45 is selected from the group consisting of Phe and Tyr; and wherein said polypeptide further comprises one to four amino acids upstream of the Kunitz domain.