Patent ID: 7575901
Filing Date: 2009-08-18
Classification: C12N,C12P

Abstract:
1. A method for producing nucleoside 5′-phosphate ester, comprising the steps of culturing a bacterium belonging to Escherichia coli having an ability to produce nucleoside 5′-phosphate ester, in which expression of ushA gene and aphA gene is decreased as compared to a wild type strain by mutating or disrupting the ushA gene and the aphA gene, in a medium to produce and accumulate nucleoside 5′-phosphate ester in a medium, and collecting the nucleoside 5′-phosphate ester from the medium, wherein the nucleoside 5′-phosphate ester is selected from the group consisting of inosine 5′-phosphate ester and guanosine 5′-phosphate ester, and wherein the 5′-nucleotidase activity in the periplasm is substantially eliminated.