Patent ID: 9157906
Filing Date: 2015-10-13
Classification: A61K,A61P,C12Q,G01N

Abstract:
1. An in vitro method of identifying a compound that inhibits phosphodiesterase 1 B (“PDE1B”) activity in a dopamine D1 receptor intracellular signaling pathway by binding to or altering the expression of PDE1B in a cell or tissue expressing dopamine D1 receptor comprising: (a) determining a first level of PDE1B activity and Thr34 of DARPP-32 phosphorylation in said cell or tissue; (b) contacting said cell or tissue with a test compound; and (c) determining a second level of PDE1B activity and Thr34 of DARPP-32 phosphorylation in said cell or tissue, wherein a difference in said first level and said second level of PDE1B activity and Thr34 of DARPP-32 phosphorylation is indicative of the ability of said test compound to modulate PDE1B activity.