Patent ID: 7049090
Filing Date: 2006-05-23
Classification: C07K,G01N

Abstract:
1. A method of identifying a compound that modulates phosphorylation of a transcription factor that functions in a hedgehog signaling pathway comprising: a) incubating components comprising the compound, a phosphorylated transcription factor that, when phosphorylated, binds to a hedgehog response element, wherein the hedgehog response element is operatively associated with a target gene, and a phosphatase, under conditions sufficient to allow the components to interact; and b) determining a change in the phosphorylation state of the transcription factor as compared to the phosphorylation state of the transcription factor prior to incubating, wherein a change in the phosphorylation state is indicative of a compound that modulates phosphorylation of a transcription factor that functions in a hedgehog signaling pathway.