Patent ID: 8404652
Filing Date: 2013-03-26
Classification: A61K,C07B,C07C,C07K,G01N

Abstract:
1. A method for inhibiting collagen accumulation in a mammal in need of such treatment, said method comprising administering to a mammalian cell(s) of said mammal an exogenous gene encoding a DNA-binding protein said DNA-binding protein having any of the following amino acid sequences so that said exogenous gene is located in a position enabling its expression in said mammalian cell(s): (a) the amino acid sequence represented by SEQ ID NO:1, which is an amino acid sequence of a protein having an ability of inhibiting the transcription of a type-I collagen gene; (b) an amino acid sequence whose sequence identity with the amino acid sequence represented by SEQ ID NO:1 is 80% or more and which is an amino acid sequence of a protein having an ability of inhibiting the transcription of a type-I collagen gene; (c) an amino acid sequence encoded by a DNA having a nucleotide sequence whose sequence identity with a DNA having a nucleotide sequence encoding the amino acid sequence represented by SEQ ID NO:1 is 90% or more and which is an amino acid sequence of a protein having an ability of inhibiting the transcription of a type-I collagen gene; and (d) an amino acid sequence encoded by a DNA capable of being hybridized under a stringent condition with a DNA having a nucleotide sequence encoding the amino acid sequence represented by SEQ ID NO:1 and which is an amino acid sequence of a protein having an ability of inhibiting the transcription of a type-I collagen gene, wherein the stringent condition comprises hybridization at 45° C. in the presence of a 6×SSC solution, and washing at 65° C. with a 0.2×SSC solution, and wherein for (b) or (c), the sequence identity is about 100% for the 52nd to 130th amino acids and at least 50% for the 1st to 51st amino acids.