Patent ID: 7939248
Filing Date: 2011-05-10
Classification: C12N

Abstract:
1. A method for producing a metabolite selected from the group consisting of 4-OH-tolbutamide, 4-OH-warfarin, 6-OH-warfarin, 7-OH-warfarin, 6-OH-chlorzoxazone, dextrorphan, 3-methoxy-morphinan, 3-hydroxy-morphinan, 7-HC sulfate, 7-HC glucuronide, 1-OH-midazolam, 4-OHmidazolam, and acetaminophen from an exogenous precursor, which metabolite is one that is produced by normal mammalian hepatocytes in vivo when the precursor is supplied, said method, comprising: (a) preparing cultured hepatocytes by (b) providing to said metabolite the precursor which is to be converted into said metabolite for a period of time sufficient to convert the precursor into said metabolite; and (c) obtaining said metabolite from the culture medium, the cells or both, thereby producing said metabolite.