Patent ID: 8398986
Filing Date: 2013-03-19
Classification: C07K

Abstract:
1. An isolated protein comprising a polypeptide exhibiting at least 70% sequence identity to the amino acid sequence of SEQ ID NO:1, wherein the protein comprises one or more mutations that reduce or eliminate ADP-ribosylating activity of the protein, wherein the one or more mutations are made to at least one of the following amino acid residues of SEQ ID NO:1: 504, 506, 537, 539, 548, 564, 567, 570, 573, 574, and 576, and further wherein (a) when the mutation is at amino acid residue 504, the mutation is either a deletion of residue 504 or a substitution with Lys; (b) when the mutation is at amino acid residue 506, the mutation is a substitution with Glu or Tyr; (c) when the mutation is at amino acid residue 537, the mutation is a substitution with Phe; (d) when the mutation is at amino acid residue 539, the mutation is a substitution with Lys or Tyr; (e) when the mutation is at amino acid residue 548, the mutation is a substitution with Lys; (f) when the mutation is at amino acid residue 564, the mutation is a substitution with Lys, Asp or Ser; (g) when the mutation is at amino acid residue 567, the mutation is a substitution with Glu; (h) when the mutation is at amino acid residue 570, the mutation is a substitution with Lys, Asp or Gln; (i) when the mutation is at amino acid residue 573, the mutation is a substitution with Lys, Asp or Gln; (j) when the mutation is at amino acid residue 574, the mutation is a substitution with Lys, Asp or Gln; and (k) when the mutation is at amino acid residue 576, the mutation is a substitution with Lys, Asp or Gln.