Patent ID: 6489124
Filing Date: 2002-12-03
Classification: C07K,G01N

Abstract:
A method of assaying a candidate ligand for binding interaction with a human NR2A protein, which comprises the steps of:(1) incubating the candidate ligand under appropriate conditions with a cell that has been mutated to produce a human NR2A protein, said cell having incorporated expressible therein a heterologous polynucleotide that encodes a modulatory protein selected from the group consisting of NR2A-1 having the amino acid sequence of SEQ ID NO:2 and NR2A-2 having the amino acid sequence of SEQ ID NO:2 wherein the lysine at position 270 is replaced by glutamic acid, or with a membrane preparation containing said NR2A protein, and then (2) determining the extent of binding between the human NR2A protein and the candidate ligand.