Patent ID: 6811966
Filing Date: 2004-11-02
Classification: G01N,Y10T

Abstract:
A process comprising(a) screening a library of small organic compounds, less than about 2000 daltons in size, with a target protein-ligand conjugate formed by the covalent bonding of a target protein comprising a first reactive functionality with a ligand that (1) comprises less than about 20 carbon atoms; (2) has affinity for interacting with a particular site on the target protein, (3) comprises a second reactive functionality and (4) comprises a free chemically reactive group, wherein the second reactive functionality of the ligand reacts with the first reactive functionality of the target protein to form a first covalent bond such that the ligand in the target protein-ligand conjugate contains a free chemically reactive group, in aqueous solution, under conditions wherein at least one member of the library forms a second covalent bond with said free chemically reactive group in the target protein-ligand conjugate, and (b) determining the identity of a small organic compound that binds covalently to the free chemically reactive group of the target protein-ligand conjugate.