Patent ID: 8372604
Filing Date: 2013-02-12
Classification: C12N,C12P,C12Q

Abstract:
1. A method for amplifying a nucleic acid molecule comprising: (a) first, contacting an RNA template with a mixture comprising a Moloney murine leukemia virus (M-MLV) reverse transcriptase and one or more DNA polymerases, thereby forming a reaction mixture that comprises the RNA template, the reverse transcriptase and the one or more DNA polymerases, wherein the unit ratio of said reverse transcriptase to said DNA polymerases in the reaction mixture is greater than about 3:2 (b) second, incubating said reaction mixture under conditions sufficient to reverse transcribe the RNA template; and (c) third, further incubating the reaction mixture under conditions sufficient to amplify a DNA molecule complementary to all or a portion of said RNA template.