Patent ID: 8942458
Filing Date: 2015-01-27
Classification: G01N

Abstract:
1. A method of distinguishing and sorting cells, comprising: irradiating living cells with light; receiving transmitted light that is transmitted through the living cells; processing the transmitted light to obtain transmitted light information; receiving at least one of side-scattering light and fluorescent light from the living cells that are irradiated with light; processing the at least one of the side-scattering light and the fluorescent light to obtain one or both of side-scatter light information and fluorescent light information; and distinguishing and sorting a specific cell mass or a part of the living cells in the specific cell mass by using the transmitted light information and one or both of the side-scattering light information and the fluorescent light information, wherein one or both of the side-scattering light information and the fluorescent light information indicates the characteristics of the internal structures of the living cells, and the transmitted light information indicates at least one of: (a) morphological characteristics of the living cells, including at least one of size and shape of the living cells, and (b) characteristics of internal structures of the living cells, wherein the specific cell mass or the part of the living cells in the specific cell mass is distinguished and sorted, regardless of a presence of fluorescent processing that includes an antibody-antigen reaction at a cell surface and a presence of an expression of fluorescent protein in a cell being performed on the part of the living cells in the specific cell mass, the part of the living cells in the specific mass includes stem cells and cells at an S phase of the cycle are distinguished using the transmitted light information, and the stem cells that are bigger than a biggest cell at the S phase are sorted by using a histogram of cells where an abscissa axis indicates the transmitted light information, or by using an analytical dispersion diagram of cells where an abscissa axis indicates the transmitted light information and a vertical axis indicates the side-scattering light information, the transmitted light information, or the fluorescent light information.