Patent ID: 6150090
Filing Date: 2000-11-21
Classification: C07K,C12N,C12Q,G01N

Abstract:
An assay for identifying a compound which modulates the activity of an NF-kB polypeptide, comprising(a) contacting a cell or cell lysate comprising an NF-kB polypetide and an IkB polypeptide with a test agent; and(b) detecting one or more of the following characteristics:(i) the level of the NF-kB polypeptide,(ii) the level of the IkB polypeptide,(iii) the level of DNA binding activity of the NF-kB,(iv) the level of expression of a gene operably linked to one or more NF-kB regulatory element,(v) in the case of the cell, the level of nuclear and/or cytoplasmic localization of the NF-kB polypeptide, or(vi) modification of the IkB polypeptide;wherein a change in one or more of said characteristics in the presence of the test agent, relative to the absence of test agent, indicates that the test agent is a compound which modulates the activity of an NF-kB polypeptide.