Patent ID: 6054276
Filing Date: 2000-04-25
Classification: C12N,C12Q,G16B

Abstract:
A method of mapping a polynucleotide, the method comprising the steps of:(a) providing a plurality of populations of restriction fragments, the restriction fragments of each population having an interior and ends defined by digesting the polynucleotide with a plurality of combinations of restriction endonucleases, and each restriction fragment being inserted into a vector;(b) cleaving each vector to remove the interior of the restriction fragment and to leave a segment of each end of the restriction fragment in the vector;(c) circularizing each vector so that the segments of each end of each restriction fragment are ligated together to form a pair of segments;(d) determining the nucleotide sequences of a sample of pairs of segments to obtain a sample of pairs of nucleotide sequences; and(e) ordering the pairs of nucleotide sequences by matching the nucleotide sequences between pairs to form a map of the polynucleotide.