Patent ID: 7968302
Filing Date: 2011-06-28
Classification: C12Q,G01N

Abstract:
1. An in vitro method for diagnosing coagulation activation linked to a risk of thromboembolic disease starting from a blood sample removed from a patient, comprising: i) measuring a quantity of fibrin degradation products contained in the sample, by measuring a quantity of D-dimers present in the sample and constituting a base level of D-dimers; ii) treating the sample by incubation with a plasminogen activator with a high affinity for fibrin (Pa-Fb sp) under conditions allowing degradation of soluble fibrin contained in the sample into degradation products without resulting in degradation of fibrinogen, and measuring a quantity of D-dimers contained in the treated sample; iii) calculating a difference between the quantity of D-dimers measured after activation by the Pa-Fb sp activator in step ii) and the quantity of D-dimers before said activation measured in step i), said difference constituting a degree of degradation of soluble fibrin (SDF); iv) comparing the level of D-dimers measured in step i) with a normal threshold value determined for said degradation product and comparing the level of SDF calculated in step iii) with a normal threshold value determined for SDF; a risk of thromboembolic disease existing if at least one of the levels of D-dimers measured in step i) or the calculated level of SDF obtained in step iii) is higher than the respective determined normal value and said risk being excluded when the level of D-dimers obtained in step i) and the level of SDF obtained in step iii) are lower than the respective normal threshold values.