Patent ID: 8211713
Filing Date: 2012-07-03
Classification: G01N

Abstract:
1. A method for determining an analyte in a sample, comprising: (a) mixing the sample and a first specific binding substance, the first specific binding substance being a substance that can specifically bind to the analyte, to form a complex of the analyte in the sample and the first specific binding substance; (b) adding microparticles having a second specific binding substance bound thereto to a mixture obtained in the step (a) and mixing therewith, the second specific binding substance being a substance that can specifically bind to the first specific binding substance; and (c) determining the extent of an agglutination reaction of the microparticles, in a mixture obtained in the step (b), thereby determining the amount of analyte in the sample, wherein the second specific binding substance is a monoclonal antibody against the first specific binding substance and wherein the extent of agglutination of the microparticles increases with an increase in the amount of analyte in a sample such that the extent of agglutination of the microparticles relates to the amount of analyte in a sample.