Patent ID: 6355241
Filing Date: 2002-03-12
Classification: A61K,A61P,C07K,C12N

Abstract:
A method of producing erythropoietin in a mammal, said method comprising the steps of:a) obtaining primary cells from a mammal; b) introducing into primary cells obtained in (a) a nucleic acid molecule construct comprising exogenous nucleic acid sequences encoding erythropoietin and nucleic acid sequences that direct expression of the exogenous nucleic acid sequences in primary cells, thereby producing primary cells that express the exogenous nucleic acid sequences encoding erythropoietin; c) culturing a primary cell that expresses the exogenous nucleic acid sequences encoding erythropoietin produced in (b) to produce a clonal cell strain of secondary cells from the primary cell; and d) introducing secondary cells produced in (c) into a mammal of the same species from which the primary cells were obtained to produce erythropoietin in the mammal.