Patent ID: 6171836
Filing Date: 2001-01-09
Classification: C12N,C12P,C12Y

Abstract:
An expression cassette comprising a heterologous DNA encoding two or more enzymes from the metabolic pathway for the bioconversion of cholesterol to hydrocortisone wherein one of the enzymes is a bovine enzyme which catalyzes the oxidation of progesterone to 17.alpha.-hydroxyprogesterone and the remaining one or more bovine or human enzymes catalyze at least one reaction selected from the group consisting of the oxidation of cholesterol to pregnenolone by bovine enzyme; the conversion of pregnenolone to progesterone by human enzyme; the oxidation of the 17.alpha.-hydroxyprogesterone to cortexolone by bovine enzyme; and the oxidation of cortexolone to hydrocortisone by bovine enzyme; and wherein the heterologous DNA is operably linked to control sequences required to express the encoded enzymes in a recombinant host.