Patent ID: 8808987
Filing Date: 2014-08-19
Classification: C12Q

Abstract:
1. A method for identifying an olfactory receptor included in one olfactory cell among a mouse olfactory receptor group A; the method comprising steps of: (a) obtaining the one olfactory cell; (b) extracting mRNA from the one olfactory cell obtained in the step (a); (c) obtaining cDNA with reverse transcriptase from the mRNA extracted in the step (b); (d) amplifying the cDNA obtained in the step (c) by a PCR method using a forward primer represented by SEQ ID: 01 and a reverse primer represented by SEQ ID: 02; wherein SEQ ID:01 is A (adenine) -T (thymine) -G (guanine) -K (guanine or thymine) -C (cytosine) -I (inosine) -T (thymine) -W (adenine or thymine) -I (inosine) -G (guanine) -A (adenine) -Y (thymine or cytosine) -M (adenine or cytosine) -G (guanine) -I (inosine) -T (thymine) -A (adenine) -Y (thymine or cytosine) -G (guanine) -T (thymine) -I (inosine) -G (guanine) -C (cytosine); and SEQ ID: 02 is T (thymine) -C (cytosine) -Y (thymine or cytosine) -T (thymine) -K (guanine or thymine) -R (guanine or adenine) -T (thymine) -T (thymine) -Y (thymine or cytosine) -C (cytosine) -T (thymine) -I (inosine) -A (adenine) -G (guanine) -R (guanine or adenine) -C (cytosine) -T (thymine) -R (guanine or adenine) -T (thymine) -A (adenine) -I (inosine) -A (adenine) -T (thymine) -I (inosine) -A (adenine) -G (guanine) -I (inosine) -G (guanine) -G (guanine) -R (guanine or adenine) -T (thymine) -T (thymine) (e) determining whether or not a gene sequence of the cDNA amplified in the step (d) is identical to one gene sequence included in gene sequences coding for olfactory receptors included in the mouse olfactory receptor group A; (f) determining that, if the gene sequence of the cDNA is identical to the one gene sequence in the step (e), the olfactory receptor included in the one olfactory cell is the olfactory receptor corresponding to the one gene sequence which is identical to the gene sequence of the cDNA in the step (e); where the mouse olfactory receptor group A includes the following mouse olfactory receptors: (Mouse olfactory receptor group A) Olfr1, Olfr8, Olfr20, Olfr22-ps1, Olfr29-ps1, Olfr30, Olfr33, Olfr44, Olfr53, Olfr76, Olfr155, Olfr156, Olfr159, Olfr209, Olfr290, Olfr291, Olfr295, Olfr314, Olfr327-ps1, Olfr329, Olfr332, Olfr361, Olfr368, Olfr373, Olfr382, Olfr437, Olfr457, Olfr472, Olfr476, Olfr480, Olfr482, Olfr484, Olfr498, Olfr502, Olfr505-ps1, Olfr508, Olfr538, Olfr544, Olfr694, Olfr695, Olfr697, Olfr706, Olfr713, Olfr715, Olfr721-ps1, Olfr735, Olfr802, Olfr877, Olfr914, Olfr921, Olfr945, Olfr951, Olfr971, Olfr988, Olfr992, Olfr994, Olfr995, Olfr1032, Olfr1082, Olfr1084, Olfr1095, Olfr1097, Olfr1120, Olfr1145, Olfr1152, Olfr1162, Olfr1170, Olfr1173, Olfr1328, Olfr1425, Olfr1502 and Olfr1504.