Patent ID: 8710247
Filing Date: 2014-04-29
Classification: C07D

Abstract:
1. A method of processing quassinoid(s) from natural substance(s) comprising: adding a first solvent to said natural substance(s); mixing said first solvent and said natural substance(s); evaporating said first solvent to yield a residue; adding water to said residue and washing with a second solvent, to yield a fraction of the second solvent and an aqueous fraction; saturating said aqueous fraction with a polarity increasing agent and extracting with ethyl acetate to yield a second residue; subjecting said second residue to crystallization to yield quassinoid(s) and said mother liquor; separating mother liquor with column chromatography; and subjecting said separated mother liquor to crystallization to yield additional crystalline quassinoid(s).