Patent ID: 8394632
Filing Date: 2013-03-12
Classification: A61K,C12M

Abstract:
1. A method of generating, ex vivo, a matrix of cells that mimics at least one phenotype of a naturally occurring mammalian tissue, the method comprising introducing stem cells, progenitor cells, mature cells or a mixture of these into a bioreactor, the bioreactor, comprising: a matrix material configured to receive cells in a first fluid, a first chamber configured to flow the first fluid including the cells, tangentially to a surface of the matrix material; a first gassing chamber operably linked to the first chamber, said gassing chamber providing gas that flows through the first gas permeable membrane; a first gas permeable membrane configured to permit a first gas to flow there through to the first fluid in the first chamber, and a second chamber configured to flow a second fluid through a second flow path to the matrix material; a second gassing chamber operably linked to the second chamber, said gassing chamber providing gas that flows through the second gas permeable membrane; a second gas permeable membrane configured to permit a first gas to flow there through to the second fluid in the second chamber, wherein the matrix material in the bioreactor is disposed between the first and second flow paths; providing a culture media to the bioreactor for a time and under conditions suitable for the cells to arrange on the matrix material of the bioreactor whereby the cells so arranged mimic at least one phenotype of a naturally occurring mammalian tissue.