Patent ID: 6635437
Filing Date: 2003-10-21
Classification: C07F,C12Q,Y10S

Abstract:
A method for detecting an analyte in a sample by a chemiluminescent assay procedure which comprises: (a) reacting a phosphatase enzyme with at least one compound of formula I to produce chemiluminescence for detecting the analyte, wherein in the compound of formula I, Het is selected from the group consisting of: wherein * indicates the point of attachment of an enol phosphate group, wherein Z is selected from the group consisting of O and S atoms, wherein R6 is selected from the group consisting of alkyl, substituted alkyl, aryl, substituted aryl and aralkyl groups, wherein each H is independently selected from H and a cationic center and wherein n is a number which satisfies electroneutrality;(b) detecting the chemiluminescence; and (c) relating the amount of the chemiluminescence to the amount of the analyte.