Patent ID: 6020141
Filing Date: 2000-02-01
Classification: B01L,C07K,C12Q,C40B,G01N,Y02A

Abstract:
A multi-variable method for ranking the affinity of one or more combinations of molecules of a multiplicity of different molecules for a target molecule which is capable of unfolding due to a thermal change, which comprises(a) contacting said target molecule with a combination of two or more different molecules of said multiplicity of different molecules in each of a multiplicity of wells in a microplate;(b) simultaneously heating said multiplicity of wells from step (a);(c) measuring in each of said wells a physical change associated with the thermal unfolding of said target molecule resulting from said heating;(d) generating a thermal unfolding curve for said target molecule as a function of temperature for each of said wells;(e) comparing each of said thermal unfolding curves in step (d) with (i) each of said other thermal unfolding curves obtained for said target molecule and to (ii) the thermal unfolding curve for said target molecule in the absence of any of said molecules in said multiplicity of different molecules; and(f) ranking the affinities of said combinations of different molecules for said target molecule according to the change in each of said thermal unfolding curves.