Patent ID: 8425901
Filing Date: 2013-04-23
Classification: C12N,C12P

Abstract:
1. An alpha 1,3 N-acetylgalactosaminyltransferase (alpha3GalNAc-T) polypeptide, wherein the polypeptide is capable of transferring a 2′ modified sugar from a sugar donor to a sugar acceptor, and wherein the polypeptide comprises: i) SEQ ID NO:21 having one or more amino acid substitutions in the donor substrate binding site, the hinge region, or the DXD motif; ii) SEQ ID NO:21 having one or more amino acid substitutions at position 191, 228, 280, 281, or 282; ii) SEQ ID NO:21 having a proline (P) at amino acid position 191; iii) SEQ ID NO:21 having a methionine (M) at amino acid position 228; or iv) any one of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 18, or SEQ ID NO: 20.