Patent ID: 6989275
Filing Date: 2006-01-24
Classification: C07D,C07H,C09B,Y10T

Abstract:
1. A method for detecting a secondary component in a sample comprising the steps of: a) contacting a sample containing or suspected to contain the secondary component to be detected with a primary component under conditions to form a complementary binding pair and wherein said primary component is labelled with a luminescent dye selected from the group consisting of cyanine, merocyanine, styryl and oxonal dyes containing at least one sulphonate or sulphonic acid attached to an aromatic nucleus which dye is reactive with said primary component, wherein said dye has a molar extinction coefficient of at least 50,000 liters per mole centimeter, quantum yield of at least 5 percent and absorbs and emits maximally in the 400 to 900 nm range; and b) detecting the binding of said secondary component to said primary component by: