Patent ID: 9187555
Filing Date: 2015-11-17
Classification: B01D,B01J,C07K

Abstract:
1. A method of separating one or more immunoglobulin containing proteins from a liquid, which method comprises: (a) contacting the liquid with a separation matrix comprising ligands immobilised to a support; (b) allowing said immunoglobulin containing proteins to adsorb to the matrix by interaction with the ligands; (c) an optional step of washing the immunoglobulin containing protein adsorbed matrix; (d) recovering said immunoglobulin containing proteins by contacting the matrix with an eluent which releases the proteins; the improvement being that each of said ligands comprises one or more monomeric domains of staphylococcal Protein A (SpA) (E, D, A, B, C) or Protein Z, wherein in at least one of the one or more monomers, the Asparagine or Histidine at the position corresponding to H18 of the B domain of Protein A or Protein Z has been substituted with a Serine, and wherein the ligand provides an increase in elution pH compared to non-substituted ligand with essentially unaltered or improved dynamic binding capacity and yield.