Patent ID: 6800477
Filing Date: 2004-10-05
Classification: C12P,Y10S

Abstract:
A process for the preparation of a compound of Formula (I) WhereinX is F, Cl, I or R1; Cl, Br, I or R1; R1 is substituted or unsubstituted alkyl, alkenyl or (CH2)nCOR2; n is an integer from 1 to 10; R2 is OH, OR3 or NH2; and R3 is substituted or unsubstituted alkyl, alkenyl, C3-7cycloalkyl or substituted or unsubstituted aryl; by stereoselective reduction of a compound of Formula (II) whereinX Is F, Cl, Br or I; and Cl, Br, I or R1; R1 is substituted or unsubstituted alkyl, alkenyl, or (CH2)nCOR2; n is an integer from 1 to 10; R2 is OH, OR3 or NH2; and R9 is substituted or unsubstituted alkyl, alkenyl, C3-7cycloalkyl or substituted or unsubstituted aryl; by reaction with an oxidoreductase enzyme capable of catalyzing the enzymatic reduction of ketones represented by Formula (II), wherein said oxidoreductase enzyme is the Pichia methanoilca ketoreductase of SEQ ID NO. 7 as expressed in Eschericha Coli.