Patent ID: 6764686
Filing Date: 2004-07-20
Classification: A61K,C07K,Y10S

Abstract:
A modified pneumolysin polypeptide comprising one or more amino acid substitutions in a wild type pneumolysin polypeptide having the amino acid sequence SEQ ID NO:3, wherein said one amino acid substitution occurs at a position selected from the group consisting of positions 61, 148, and 195, or wherein said more than one amino acid substitutions occur at positions selected from the group consisting of 17, 18, 33, 41, 45, 46, 61, 63, 66, 83, 101, 102, 128, 148, 189, 195, 239, 243, 255, and 257, and wherein said modified pneumolysin polypeptide is soluble, elicits antibodies which are cross-reactive with wild-type pneumolysin, and has attenuated hemolytic activity.