Patent ID: 7288368
Filing Date: 2007-10-30
Classification: G01N,Y10S

Abstract:
1. A membrane-receptor reagent for measuring the interaction between test analytes and target membrane receptors, comprising: a support containing an energy-emitting surface that emits energy that varies as a function of distance from said surface; tethers covalently or non-covalently bound to said support; reagent ligands attached to said tethers; a fluid lipid membrane in the form of a liposome phospholipid vesicle, or lipid bilayer mounted on a second surface that projects away from said energy emitting surface, comprising target membrane receptors that bind to target analytes and reversibly bind to said reagent ligands, wherein said target membrane receptors move fluidly within said fluid lipid membrane and further comprise integral, peripheral, or transmembrane proteins labeled with a moiety that produces a detectable signal upon receiving excitation energy; and anchoring means for irreversibly binding said fluid lipid membrane to said support; wherein binding between said target membrane receptors and target analyte either blocks or promotes binding between said reagent ligands and said target membrane receptors, causing a change in an average distance between said target membrane receptors and said energy emitting surface, resulting in a change in said detectable signal.