Patent ID: 8753856
Filing Date: 2014-06-17
Classification: C12N,C12P,Y02P

Abstract:
1. A process for preparing a stable and viable biocatalyst; wherein said process comprises: (a) preparing Cross Linked Enzyme Aggregates from an enzyme by either: (b) entrapping the Cross Linked Enzyme Aggregates in a solution of 10% w/w polyvinyl alcohol and 10% w/w gelatin at a temperature of 5° C.; (c) maintaining said solution of 10% w/w polyvinyl alcohol and 10% w/w gelatin at a temperature of 5° C. for 12 hours to obtain a gelled solution; (d) comminuting said gelled solution to produce gel aggregates having a particle size of between 100 and 300 microns; and (e) recrosslinking the gel aggregates with glutaraldehyde at 30° C. at a pH of between 7.5 and 7.6 to improve structural stability, thereby obtaining said biocatalyst as a product.