Patent ID: 8835127
Filing Date: 2014-09-16
Classification: C12M,C12Q,G01N

Abstract:
1. A method of detecting a nucleic acid sequence in a sample comprising the steps of: a) combining the sample with at least one antigen-conjugated oligonucleotide under conditions suitable for hybridization of the antigen-conjugated oligonucleotide to the nucleic acid sequence; b) adding an emittor cell comprising one or more receptors and an emittor molecule, wherein the one or more receptors bind to the antigen of the antigen-conjugated oligonucleotides and results in an increase in intracellular calcium, and said emittor molecule emits a photon in response to the increase in intracellular calcium; and c) measuring photon emission from the emittor cell, thereby detecting a nucleic acid sequence in a sample.