Patent ID: 8764828
Filing Date: 2014-07-01
Classification: A61F,A61K,A61L,C12N

Abstract:
1. A system for forming a bone-ligament construct, comprising: a substrate; bone marrow stromal cells provided on the substrate without disposing the cells in an exogenous scaffold; a fibrogenic growth medium provided in contact with the bone marrow stromal cells which causes the cells to generate an extracellular matrix when cultured in vitro and to self-organize to form a confluent ligament monolayer; and at least one bone construct secured to or configured to be secured to the confluent monolayer in co-culture in a fibrogenic differentiation medium, with the ligament monolayer and the bone construct maintaining their respective differentiated states while creating a functionally integrated tissue interface therebetween, wherein a three-dimensional bone-ligament construct is formed via detachment of the monolayer from the substrate to at least partially surround the at least one bone construct; wherein the fibrogenic growth medium comprises a ligament growth medium and the fibrogenic differentiation medium comprises a ligament differentiation medium; and wherein the ligament differentiation medium includes a lower serum concentration compared with the ligament growth medium.