Patent ID: 7214490
Filing Date: 2007-05-08
Classification: C12Q

Abstract:
1. A method of genotyping an individual comprising: providing a first nucleic acid sample from said individual; obtaining a second nucleic acid sample by: fragmenting said first nucleic acid sample to form fragments; ligating an adaptor to one end of said fragments; exposing said fragments to a collection of selection probes, each of said selection probes comprising a single stranded region that is complementary to a region that is known or believed to contain a polymorphism and further comprising a unique sequence at the 5′ end, under conditions to allow for hybridization between selection probes and target sequences to form a plurality of probe-target complexes; extending said target sequences in said probe-target complexes using the selection probe in the probe-target complex as template so that each target sequence will be extended at the 3′ end to contain a sequence that is complementary to the unique sequence at the 5′ end of the selection probe; and amplifying the extended target sequences using a first primer to said adaptor and a second primer to said unique sequence at the 5′ end of the selection probe, thereby obtaining said second nucleic acid sample; providing a nucleic acid array wherein said array comprises probes designed to interrogate polymorphisms; hybridizing said second nucleic acid sample to said array; generating a hybridization pattern resulting from said hybridization; and determining the presence or absence of said polymorphism in the individual based upon an analysis of the hybridization pattern.