Patent ID: 7544793
Filing Date: 2009-06-09
Classification: C07H,C12P,C12Q

Abstract:
1. A method for simultaneously producing two full-length duplex nucleic acid polymers which have identical sequences on a solid surface comprising multiple sites wherein each of the two full-length duplex nucleic acid polymers is located on a different site on the solid surface and wherein each duplex consists of a first strand and a second complementary strand each strand having a 5′ end and a 3′ end comprising: (a) placing a first capture probe of a defined length which is identical to a portion of the 3′ end of the first strand on a first site on the solid surface; (b) placing a second capture probe of a defined length which is identical to a portion of the 3′ end of the second strand on a second site on the solid surface; (c) contacting the solid surface with a pool of partially double-stranded oligonucleotides, which comprise overlapping fragments of the full length duplex nucleic acid polymers to be produced; (d) hybridizing the first and second capture probes with the pool of partially double-stranded oligonucleotides such that first and second full-length duplex nucleic acid polymers are formed in which there are nicks and gaps in the duplexes; (e) washing the first and second full-length duplex nucleic acid polymers under conditions to remove nucleic acid polymers which do not have sequences which are identical to the two full-length duplex nucleic acid polymers; and (f) ligating and extending the first gapped and unligated full-length duplex nucleic acid and the second gapped and unligated full-length duplex nucleic acid to produce two full-length duplex nucleic acid polymers which have identical sequences wherein the first full-length duplex nucleic acid polymer is attached to the first site through the 3′ end of the first strand and the second full-length duplex nucleic acid polymer is attached to the second site through the 3′ end of the second strand.