Patent ID: 7630836
Filing Date: 2009-12-08
Classification: C07K,C12Q

Abstract:
1. A method for identifying a genomic polynucleotide which is concerned with biosynthesis of an antibiotic and is homologous to a gene derived from an actinomycetes, said method comprising: (a) incubating a polynucleotide array with a labeled genomic polynucleotide, under hybridization conditions, said polynucleotide array comprising a solid support adhered to at least two of the following groups of polynucleotides (1), (2) and (3): (1) first polynucleotides comprising the nucleotide sequence represented by SEQ ID NO:415, (2) second polynucleotides which are 80% homologous to the first polynucleotides and which encode a polyketide synthase, and (3) third polynucleotides comprising a sequence of 10 to 200 continuous bases of the first or second polynucleotides, wherein at least one polynucleotide on the array is selected from a different group from that of other polynucleotides on the array (b) detecting any hybridization of said genomic polynucleotides to said array, and (c) determining whether hybridized genomic polynucleotides of step (b) are concerned with biosynthesis of an antibiotic and are homologous to a gene derived from an actinomycetes.