Patent ID: 6403957
Filing Date: 2002-06-11
Classification: B01J,B01L,B82Y,C07B,C07C,C07D,C07H,C07K,C08G,C12Q,C40B,G01N,G03F,G11C,H01L,Y02P,Y10S,Y10T

Abstract:
A polynucleotide analysis apparatus comprising:a substrate comprising a plurality of different polynucleotides, each of said different polynucleotides occupying a different location of said substrate, each of said locations having an area of 10âˆ’1 cm2 or less, said substrate comprising more than 10 of such polynucleotides, at least some of said polynucleotides bearing fluorescently labeled target molecules excitable with a laser; an argon laser light source for directing light at said surface of said substrate, said argon laser lightsource generating light at a frequency to excite said fluorescently labeled target molecules, said frequency different from a frequency of light generated by said fluorescently labeled target molecules; a lens system to focus light on said substrate; a beam deflector for directing light to said surface at a right angle to said substrate; a CCD detector for detecting light fluoresced from said substrate in response to said light from said flurorescently labeled target molecules, said light fluoresced at a wavelength greater than 488 nm; a translator for scanning light on said substrate whereby a plurality of known locations of said substrate are exposed to light to read a region of said substrate; and a data collection, recording and management system for storing fluoresced light intensity as a function of location on said substrate, said data collection, recording and management system coupled to said translator and said CCD detector, said data collection, recording and management system storing light intensity as a function of location on said substrate and translating said light intensity as a function of location into nucleic acid sequence information.