Patent ID: 8877477
Filing Date: 2014-11-04
Classification: B01D,B01J,C07K,C08F,C08H,C08J,C08L

Abstract:
1. A method for isolating proteins from a solution containing the proteins the method including: a. providing polymeric ion exchange resins comprising hydroxylic polymer particles functionalized with a temperature-responsive copolymer, including a proportion of ionizable chemical groups, wherein the copolymer is propagated on the hydroxylic polymer particles and the polymeric ion exchange resins do not comprise a silica matrix; b. contacting the solution containing the proteins with the polymeric ion exchange resins at a temperature between 30° C. and 80° C. to facilitate retention of the proteins by the crosslinked polymer particles; c. replacing the solution containing the protein with a rinse solution; d. replacing the rinse solution with a release solution, wherein the temperature of the release solution is lower than the temperature at which the solution containing the proteins was contacted with the crosslinked polymer particles and is effective for releasing the proteins from the crosslinked polymer particles; and e. isolating the release solution containing the protein.