Patent ID: 9187554
Filing Date: 2015-11-17
Classification: C07K,C12N

Abstract:
1. A coryneform bacterium having an ability to produce a heterologous protein by secretory production, wherein said bacterium is modified to have reduced activities of a penicillin-binding protein and a cell surface layer protein, and wherein the penicillin-binding protein is a PBP1 a protein selected from the group consisting of: (A) a protein comprising the amino acid sequence of SEQ ID NO: 82, (B) a protein comprising an amino acid sequence of SEQ ID NO: 82, but which includes substitution, deletion, insertion, or addition of 1 to 10 amino acid residues, and wherein said protein has a property that if the protein activity is reduced in the coryneform bacterium, the amount of the heterologous protein produced by secretory production is increased compared with that observed for a non-modified strain; and wherein the cell surface layer protein is a CspB protein selected from the group consisting of: (A) a protein comprising the amino acid sequence of SEQ ID NO: 98, (B) a protein comprising an amino acid sequence of SEQ ID NO: 98, but includes substitution, deletion, insertion, or addition of 1 to 10 amino acid residues, and wherein said protein has a property that if the protein activity is reduced in the coryneform bacterium, the amount of the heterologous protein produced by secretory production is increased compared with that observed for a non-modified strain.