Patent ID: 8993246
Filing Date: 2015-03-31
Classification: C12Q,G01N

Abstract:
1. A bioassay method for detecting and/or quantitating an analyte comprising contacting said analyte with a first group and a second group, said first group comprising a non-luminescent lanthanide ion carrier chelate covalently linked to a first recognition element specific for said analyte, wherein said non-luminescent lanthanide ion carrier chelate comprises a lanthanide ion carrier ligand bound a lanthanide ion and said second group comprising an antenna ligand covalently linked to a second recognition element specific for said analyte; wherein said lanthanide ion carrier chelate is an ionic macrocyclic chelate structure selected from the group consisting of pentadentate, hexadentate, heptadentate and octadentate; wherein said antenna ligand binds to said lanthanide ion and the antenna ligand is either monodentate, bidentate, tridentate or tetradentate, and the combination of ion carrier chelate and antenna ligand is selected so that the sum total of ligand coordination sites is nine or ten; and recognizing said analyte by said first recognition element of said first group and by said second recognition element of said second group, such that chelate complementation by formation of a mixed lanthanide chelate complex through complementation of said non-luminescent lanthanide ion carrier chelate carrying said lanthanide ion with said antenna ligand occurs that results in increased fluorescence.