Patent ID: 8557590
Filing Date: 2013-10-15
Classification: G01N,Y10T

Abstract:
1. A method for measuring glycated hemoglobin, the method comprising: a hemolysis step of hemolyzing a blood sample with a hemolysate; a reaction step of reacting the hemolyzed blood sample with bead conjugates in which beads are conjugated with glycated hemoglobin binding materials; a first measuring step of measuring the amount of total hemoglobin in the reacted blood sample; an isolation step of isolating normal hemoglobin from glycated hemoglobin bound to the bead conjugates, wherein the glycated hemoglobin bound to the bead conjugates is isolated from normal hemoglobin in the reacted blood sample; a second measuring step of measuring the amount of glycated hemoglobin isolated from the normal hemoglobin; and a calculation step of determining the percentage of the glycated hemoglobin in the blood sample on the basis of the measured amounts of total hemoglobin and glycated hemoglobin, wherein the isolation step is performed by absorbing the normal hemoglobin using an absorption pad which is a porous pad having pores, each of which has a size greater than the size of the normal hemoglobin and smaller than the size of the bead.