Patent ID: 6248545
Filing Date: 2001-06-19
Classification: G01N,Y10T

Abstract:
A solid phase sandwich assay procedure for assaying MDA modified lipoprotein in a body fluid sample which comprises immobilizing one of a pair of antibodies on an insoluble carrier to form an immobilized antibody on said carrier and labeling the other antibody of said pair with a labeling substance wherein one antibody of said pair recognizes human MDA modified lipoprotein and the other antibody of said pair recognizes an apolipoprotein component of said lipoprotein;adding surfactant to said body fluid sample to form a mixture of body fluid sample and surfactant whereby said surfactant exposes an unexposed modified site on any MDA modified lipoprotein contained in said mixture;contacting said pair of antibodies with said mixture whereby an immune reaction occurs; anddetecting the presence of MDA modified lipoprotein bound to said insoluble carrier.