Patent ID: 7129048
Filing Date: 2006-10-31
Classification: B82Y,C12Q

Abstract:
1. A kit comprising: a substrate attached to an unlabeled probe polynucleotide comprising first and second complementary regions and a third region located between the first and second complementary regions, wherein the probe polynucleotide can form a stem-loop structure in which the first and second complementary regions hybridize to each other to form a stem and the third region forms a loop, wherein at least a part of the third region is complementary to at least a part of a capture sequence of an amplification product from a target polynucleotide, wherein the unlabeled probe polynucleotide can preferentially hybridize to the amplification product and thereby disrupt formation of the stem-loop structure under at least one set of hybridization conditions; a reagent for incorporating a label into the amplification product; a housing for retaining the substrate and the reagent; and instructions provided with said housing that describe how to use the components of the kit to assay a sample for the amplification product.