Patent ID: 9023990
Filing Date: 2015-05-05
Classification: B01J,C07K

Abstract:
1. A method for isolating and purifying recombinant human serum albumin from transgenic rice grain, sequentially consisting of the steps of: 1) subjecting a crude extract from the transgenic rice grain containing the recombinant human serum albumin to cation exchange chromatography to obtain primary product I; 2) subjecting the primary product I to anion exchange chromatography to obtain secondary product II that contains the recombinant human serum albumin; and 3) subjecting the secondary product II to hydrophobic chromatography to obtain the purified recombinant human serum albumin: wherein the secondary product II that contains the recombinant human serum albumin to be purified by hydrophobic chromatography further comprises ammonia sulfate at a concentration from 0.1 M to 1 M, and step 3) comprises subjecting the secondary product II to hydrophobic chromatography under a condition that the recombinant human serum albumin does not absorb to the resins of the hydrophobic chromatography and recovering non-adsorbed flow-through fractions to obtain the purified recombinant human serum albumin having a purity of more than 99% (monomer plus dimer and polymer) by HPLC.