Patent ID: 9040237
Filing Date: 2015-05-26
Classification: B01J,B01L,B82Y,C12Q,G01N

Abstract:
1. A method for sequencing DNA, comprising, providing an array comprised of a plurality of cavities to hold reactants, the cavities having a surface for attachment of DNA and coupled electronic sensors for detecting chemical reaction product changes in a solution, wherein each cavity is coupled to an electronic sensor; attaching DNA molecules to be sequenced to the surfaces of the cavities so that statistically only one DNA molecule to be sequenced is attached in each cavity; hybridizing a DNA oligo to each of the DNA molecules to be sequenced, the DNA oligo comprising at its 3′-end a first nuclease-resistant nucleotide or a first nuclease-resistant nucleotide analog; providing a first set of reactants to the cavities, the reactants comprising: identifying the base by monitoring increases in products of extension and/or deconstruction with the electronic sensors in the cavities; attaching the DNA oligo with a second nuclease resistant nucleotide or a second nuclease-resistant nucleotide analog, that is complementary to the base; repeating said providing reactants to the cavities and determining the identity of the single nucleotide at least one more time; and determining sequence information for the DNA molecules to be sequenced based on increases in the products of extension and/or deconstruction monitored by the electronic sensors.