Patent ID: 8050873
Filing Date: 2011-11-01
Classification: C07K,C12N,C12Q,G01N

Abstract:
1. A method for identifying candidate compounds that bind to Hypoxia Inducible Factor-1 alpha prolyl hydroxylase (EGLN1), comprising the steps of: a) generating a three-dimensional model of an EGLN1 polypeptide, wherein the three-dimensional model has the atomic coordinates as shown in Table 2; b) identifying amino acid residues forming a catalytic domain of EGLN1 from the three-dimensional model of (a), wherein the catalytic domain comprises amino acid residues Ile-256, Tyr-283, Met-299, Gly-307, Tyr-310, His-313, Asp-315, Tyr-329, Leu-343, His-374, Val-376, Arg-383, and Trp-389, wherein the amino acid residues have the atomic coordinates in Table 2; c) generating a three-dimensional model of the catalytic domain of EGLN1; d) using the three-dimensional model of the catalytic domain of EGLN1 to design or select a candidate compound that binds to EGLN1; and e) evaluating the ability of the compound to bind EGLN1 in an in vitro, in vivo, or ex vivo assay.