Patent ID: 6143877
Filing Date: 2000-11-07
Classification: C07H,C12Q

Abstract:
A method of forming a triplex from a duplex nucleic acid having in one strand thereof a target sequence that consists essentially of nucleotides of purine bases and from an oligonucleotide that includes a substantially continuous sequence of at least 6 nucleotides, which sequence is complementary in the triplex forming sense pursuant to the G/T or A/G recognition motif to the target sequence in the duplex nucleic acid, at least one guanine base containing nucleotide in the substantially continuous sequence of the oligonucleotide being replaced by the nucleotide of 6-amino-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one, the process comprising the step of:contacting the oligonucleotide with the duplex nucleic acid.