Patent ID: 8686376
Filing Date: 2014-04-01
Classification: B01L,G01N,G02B

Abstract:
1. A system comprising a characterization system and a substrate for use with a detector for detecting a luminescence response from each of a plurality of sub-regions of the substrate and for characterizing a plurality of analytes in a sample contacted with the substrate, the characterization system comprising: an aperture array comprising a plurality of apertures adapted for limiting at least one of a plurality of excitation radiation sub-beams and luminescence radiation sub-beams; and a lens array comprising a plurality of lenses corresponding with the plurality of apertures, the lens array being at a first substrate surface and being adapted for focusing excitation radiation sub-beams on a second substrate surface at the opposite side of the first substrate surface, the second side of the substrate being adapted for being contacted with the sample, each lens of said lens array being configured to: receive an excitation radiation sub-beam from a corresponding aperture of said aperture array and for directly focusing the excitation radiation sub-beam onto a sub-region of the substrate, and directly collect a luminescence radiation sub-beam from a sub-region of the substrate and guiding the luminescence radiation sub-beam to a corresponding aperture of said aperture array, wherein the characterization system further comprises: a sample holder configured to provide the substrate in proximity of the lens array and an immersion fluid configured to guide the excitation sub-beams from the lenses in the lens array to said sub-regions of the substrate or to collect the luminescence radiation sub-beams from the sub-regions of the substrate to the lenses in the lens array, wherein the immersion fluid is confined to be in front of the lenses and in front of the first substrate surface so as not to interfere with the sample; and wherein the characterization system furthermore comprises a scanner configured to provide a relative movement between the excitation radiation sub-beams and the substrate for providing a relative scanning movement of each diffraction limited spot within a corresponding sub-region on the substrate.