Patent ID: 8492099
Filing Date: 2013-07-23
Classification: C12Q,G01N

Abstract:
1. A method of measuring the immunological activity of Der p 1 in a immunogenic preparation comprising the allergenic enzyme Der p 1 and optionally one or more further allergenic enzyme(s) and an oxygen-containing metal salt adjuvant, wherein the preparation is in the form of a mixture comprising a liquid phase and a solid phase, and wherein at least a part of the allergenic enzyme(s) is adsorbed to the solid phase, the method comprising the steps of measuring the enzymatic activity of Der p 1 in the mixture, and/or measuring the enzymatic activity of Der p 1 in the liquid phase upon a separation of the liquid phase from the solid phase, and/or measuring the enzymatic activity of Der p 1 in the solid phase upon a separation of the liquid phase from the solid phase, in an enzyme activity assay wherein Z-Leu-Leu-Glu-MCA is used as a specific substrate for measuring the enzymatic activity of Der p 1, and using the measurement obtained as an indication of the immunological activity of the preparation, wherein the oxygen-containing metal salt adjuvant is aluminum hydroxide.