Patent ID: 6207371
Filing Date: 2001-03-27
Classification: A01K,C12N

Abstract:
A collection of cultured eucaryotic cells made by a process comprising:a) treating a first group of cells with a first vector to stably integrate into the genome of said cells, said first vector mediates the splicing of a foreign exon internal to a cellular transcript and said first vector comprising:a foreign exon;a splice acceptor sequence operatively positioned 5' to the foreign exon;a splice donor site operatively positioned 3' to said foreign exon; anda sequence comprising a nested set of stop codons in each of the three reading frames located between the 3' end of said foreign exon and said splice donor site;b) treating a second group of cells with a second vector to stably integrate into the genome of said cells, said second vector mediates the splicing of a foreign exon 5' to an exon of a cellular transcript and said second vector comprising:a foreign exon;a promoter element operatively positioned 5' to said foreign exon;a splice donor site operatively positioned 3' to said foreign exon; anda mutagenic foreign polynucleotide sequence positioned upstream to said promoter; andc) selecting for cells from the first group of cells and/or the second group of cells that express the products encoded by the foreign exon; whereby in the method a collection of cultured eucaryotic cells is made.