Patent ID: 6455249
Filing Date: 2002-09-24
Classification: C12Q,Y02P

Abstract:
A method for detecting predetermined nucleic acid sequences of a target molecule comprising the steps of:hybridizing a deoxyoligomer probe molecule to a target molecule to form a complex wherein the complex has at least one site of basepair mismatch or basepair being modified; reacting the complex with a mismatch repair enzyme to remove an A base, a T base, or other modified bases from the deoxyoligomer probe at the site of basepair mismatch to form an apurinic or apyrimidinic site; and binding an amplifier molecule to the apurinic or apyrimidinic site to cleave a phosphodiester bond from a deoxyribose to form a probe fragment, and freeing said mismatch repair enzyme and target from the substrate complex.