Patent ID: 9096902
Filing Date: 2015-08-04
Classification: C12Q

Abstract:
1. A method for uniquely labeling a cancer gene of interest in an individual cell nucleus, comprising the steps of: (a) obtaining and providing a single cell from a patient; (b) treating the single cell and fixing it to a substrate for analysis to increase accessibility of a target chromosome and to reduce nonspecific binding; (c) hybridizing a set of three probes to uniquely label the cancer gene of interest in a target chromosome, wherein a first probe is selected to hybridize to the region proximal to the cancer gene on the target chromosome, the second probe is selected to hybridize to the cancer gene of interest, and the third probe is selected to hybridize to the region distal to the cancer gene of interest on the target chromosome, wherein the set of the three probes provides a unique label for the cancer gene of interest in the individual cell nucleus, and wherein the cancer gene of interest is at least one of Erbbl, Erbb2, HIPK2, PTEN and XPG genes in tumor cells.