Patent ID: 7067295
Filing Date: 2006-06-27
Classification: C12N,C12Q,C12Y

Abstract:
1. An isolated protein comprising a variant of a glucose dehydrogenase consisting of the amino acid sequence of SEQ ID NO:1, wherein said variant glucose dehydrogenase has pyrrolo-quinoline quinone as a coenzyme, wherein said variant continues to have glucose dehydrogenase activity and said variant consists of at least one amino acid substitution selected from the group consisting of: (1) serine at position 207 substituted with an amino acid residue selected from the group consisting of lysine, asparagine, aspartate, histidine, methionine, leucine, and cysteine; (2) glutamine at position 185 substituted with lysine; (3) glutamate at position 186 substituted with lysine; (4) aspartate at position 396 substituted with lysine; and (5) alanine at position 397 substituted with aspartate.