Patent ID: 6811970
Filing Date: 2004-11-02
Classification: C12Q,G01N

Abstract:
A method for measuring heterodimerization of HIV RT, which comprises the steps of:a) providing a first solution comprising p66 subunit homodimers in the presence of a dissociation agent; b) contacting said first solution with p51 RT subunits and incubating in the presence of a reassociation buffer to allow association of a reconstituted RT complex of p66/p51 RT subunits, wherein one of said subunits comprises an affinity tag and the other of said subunits comprises a detectable label; c) contacting the incubate of step b) with an affinity medium under conditions that enable the p66/p51 complex to bind to said affinity medium; and d) determining the amount of complex formed by measuring the level of detectable label bound to the affinity medium.