Patent ID: 7582488
Filing Date: 2009-09-01
Classification: G01N

Abstract:
1. A method of detecting particular binding molecules in a sample, comprising: providing a set of encoded beads wherein, said beads having a core and a shell, where the shell is an ionic hydrogel composed of 6% hydroxyethyl methacrylate, 20% methacrylic acid and 74% methyl methacrylate by weight; immobilizing, without covalent bonding, Protein L in the ionic hydrogel using PBS as a buffer where the pH is 3 such that said Protein L is absorbed and trapped in the ionic hydrogel; suspending the encoded beads with immobilized Protein L therein, in a PBS buffer of pH 7.2 containing 0.1% BSA and 0.1% sodium azide by weight; contacting the beads with said binding molecules; and determining whether binding of said binding molecules to said Protein L takes place to thereby detect said particular binding molecules in the sample based on detection of signal from labeling associated with the binding event.