Patent ID: 8937170
Filing Date: 2015-01-20
Classification: C11D,C12N

Abstract:
1. An expression vector, comprising a nucleic acid sequence selected from the group consisting of: (a) a nucleic acid sequence encoding a polypeptide exhibiting serine protease activity and comprising the amino acid sequence set forth in SEQ ID NO:15; (b) a nucleic acid sequence encoding a polypeptide that comprises an amino acid sequence that exhibits serine protease activity and is at least 92% identical to the amino acid sequence set forth in SEQ ID NO:15, wherein sequence identity is determined using ClustalW alignment using matrix: BLOSUM, Gap Open: 10, and Gap Extension: 0.5, and wherein the amino acid sequence has the serine protease specificity of SEQ ID NO:15; (c) a cDNA sequence comprising the coding sequence of the nucleotide sequence set forth in SEQ ID NO:10, wherein the polypeptide encoded by the nucleotide sequence, in its mature form, exhibits serine protease activity; (d) a cDNA sequence comprising the coding sequence of the nucleotide sequence set forth in SEQ ID NO:12, wherein the polypeptide encoded by the nucleotide sequence, in its mature form, exhibits serine protease activity; (e) a cDNA sequence comprising the coding sequence of the nucleotide sequence set forth in SEQ ID NO:14, wherein the polypeptide encoded by the nucleotide sequence exhibits serine protease activity; (f) a cDNA sequence comprising the coding sequence of the nucleotide sequence in plasmid pALK2529 contained in the (g) a nucleic acid sequence the coding sequence of which differs from the coding sequence of the nucleotide sequence of any one of (c) to (f) due to the degeneracy of the genetic code; (h) a nucleic acid sequence comprising the nucleotide sequence set forth in SEQ ID NO:12 fused at its 5′ end to the nucleotide sequence of a heterologous signal sequence, the nucleic acid sequence encoding a polypeptide, which in its mature form, exhibits serine protease activity; and (i) a nucleic acid sequence, that hybridizes under stringent conditions with the complement of the nucleic acid sequence contained in DSM 22171 and encodes a polypeptide comprising an amino acid sequence that exhibits the serine protease specificity of SEQ ID NO:15 and is at least 92% identical to the amino acid sequence set forth in SEQ ID NO:15, wherein sequence identity is determined using ClustalW alignment using matrix: BLOSUM, Gap Open: 10, and Gap Extension: 0.5, and wherein stringent conditions comprise hybridization in a solution comprising 6×SSC, 5×Denhardt's reagent, and 0.5% SDS at 65° C., and washing twice for 15 minutes at 65° C. in a solution comprising 0.1×SSC and 0.1% SDS.