Patent ID: 7615370
Filing Date: 2009-11-10
Classification: B01L

Abstract:
1. A method for preventing air bubbles in an essentially gap-shaped hybridization chamber of a system for hybridizing nucleic acid samples, proteins, or tissue sections that are immobilized on slides, the method comprising the steps of: (a) providing an essentially gap-shaped hybridization chamber between a slide and a cover, the cover being positioned in relation to the slide in such a way that the hybridization chamber is sealed to surrounding air; (b) essentially filling the hybridization chamber as provided in step (a) with a liquid; (c) building-up a chamber pressure in the hybridization chamber with a pressure device of said system; and (d) keeping the chamber pressure at a pressure of at least 100 mbar above the normal atmospheric pressure existing in the surrounding air during a hybridization procedure.