Patent ID: 9212373
Filing Date: 2015-12-15
Classification: C12N,Y02A

Abstract:
1. A gene expression system for controllably inhibiting the accumulation in a plant cell of ethylene inducible proteins comprising: an activation cassette comprising, under control of a constitutive G10-90 promoter and in operative association therewith, (a) a GAL 4 DNA-binding domain (DBD) that recognizes a response element comprising five copies of GAL4 response element; (b) an ecdysone receptor ligand binding domain (EcRLBD); and (c) a VP16 activation domain (AD) which is activated in the presence of an inducing composition; and a target cassette comprising (d) an inducible promoter comprising, in operative association, the five copies of the GAL 4 response element to which the DBD of (a) binds and the minimal 35 S promoter responsive to activation of the VP16 AD, the inducible promoter controlling expression of (e) a nucleic acid sequence that encodes an ETP1 or ETP2 protein; said activation cassette and the target cassette in operative association to enable interaction, when in a plant cell, with an inducing composition, to controllably increase expression of the ETP1 or ETP2 protein to inhibit the accumulation of the EIN2 gene product in the plant in the presence of ethylene, the inhibition of said accumulation in EIN2 protein expression controllable by the timing, the concentration, and the duration of the application of the inducing composition.