Patent ID: 6221638
Filing Date: 2001-04-24
Classification: C12N,C12P,Y10S

Abstract:
A method for preparing an optically active (R)-amino compound comprising the step of stereoselectively carrying out amino group transfer by action of an (R)-form-specific transaminase in the co-presence of a ketone compound (amino acceptor) and an amino compound (amino donor), wherein said amino acceptor is a compound represented by the following general formula (I): ##STR6##wherein n is 0 to 5; m is 0 or 1; and X represents an unsubstituted aryl group having 6 to 14 carbon atoms; an aryl group having 6 to 14 carbon atoms and having one or more substitutents selected from the group consisting of an alkyl group having 4 to 15 carbon atoms, a hydroxyl group, a fluorine atom, a chlorine atom, a bromine atom, an iodine atom, a nitro group, a carboxyl group, and a trifluoromethyl group; a substituted phenyl group selected from the group consisting of 2-methoxyphenyl, 3-methoxyphenyl, 4-methoxyphenyl, 2,4-dimethoxyphenyl, 3,4-dimethoxyphenyl and 3-trifluoromethylphenyl; a methoxyl group; a pyridyl group; a pyrazinyl group; a thienyl group; a furyl group; or a thiazolyl group; and wherein said amino donor is a compound of an achiral form, a racemic form, or an (R)-form represented by the general formula (II): ##STR7##wherein X.sub.1 and X.sub.2 independently represent a hydrogen atom; a straight-chain alkyl group having 1 to 10 carbon atoms; a branched alkyl group having 5 to 12 carbon atoms; an unsubstituted aryl group having 6 to 14 carbon atoms; an aryl group having 6 to 14 carbon atoms and having one or more substitutents selected from the group consisting of an alkyl group having 1 to 4 carbon atoms, a hydroxyl group, a fluorine atom, a chlorine atom, a bromine atom, an iodine atom, a nitro group, and a carboxyl group; an aralkyl group having 7 to 16 carbon atoms and having one or more substitutents selected from the group consisting of an alkyl group having 1 to 4 carbon atoms, a hydroxyl group, a fluorine atom, a chlorine atom, a bromine atom, an iodine atom, a nitro group, and a carboxyl group; or a hydroxymethyl group, thereby giving an optically active (R)-amino compound represented by the general formula (IV): ##STR8##wherein n, m, and X have the same definitions as those of n, m, and X in the general formula (I), respectively.