Patent ID: 6284491
Filing Date: 2001-09-04
Classification: A61K,A61P,C07K,G01N

Abstract:
A method for producing recombinant human TSH comprising the steps of:a) transfecting a eukaryotic cell with an expression vector, wherein said expression vector comprises a contiguous genomic fragment of the human thyrotropin (TSH) .beta.-subunit gene wherein the fragment comprises both coding exons of the TSH .beta.-subunit gene separated by the intron;b) culturing said transfected cell under conditions that allow said vector to direct the machinery endogenous in said eukaryotic cell to perform the splicing necessary for expression of mRNA to bring about the production of TSH .beta.-subunit and that allow for the production of TSH .alpha. subunit for combination therewith; andc) recovering substantially pure recombinant human TSH.