Patent ID: 6534281
Filing Date: 2003-03-18
Classification: G01N,Y10S,Y10T

Abstract:
A method for measuring human C-peptide comprising the steps of:(i) reacting human C-peptide contained in a sample, a first anti-human C-peptide antibody or an antigen-binding fragment thereof, and a second anti-human C-peptide antibody or an antigen-binding fragment thereof immobilized on a solid support to form an immune complex among three components; (ii) separating the formed immune complex from non-reacted antibodies and/or antigen-binding fragments thereof, and sample, and (iii) quantifying the separated immune complex; said first antibody recognizing an epitope existing in the region from residues 1-10 of SEQ ID NO:2 from the N-terminal of said human C-peptide; said second antibody recognizing an epitope existing in the region from residues 1-16 of SEQ ID NO:2 from the N-terminal of said human C-peptide; and said first and second antibodies recognizing different epitopes so that they can simultaneously bind to said human C-peptide.