Patent ID: 7262058
Filing Date: 2007-08-28
Classification: G01N,Y10S

Abstract:
1. A method of extracting lipid associated sialoprotein from cerebrospinal fluid of human subjects having or suspected of having cancer, and determining the amount of lipid associated sialoprotein in a sample of such fluid which comprises the following steps: a) adding to the sample a mixture of a chlorinated lower alkyl hydrocarbon and a lower alkyl alcohol; b) mixing the resulting admixture for a suitable period of time to dissolve lipid-bound sialic acid in the sample in the chlorinated hydrocarbon/alcohol mixture; c) centrifuging the mixture at about 6000 rpm to form a substantially clear upper phase; d) separately recovering from the clear upper phase so formed a predetermined volume of the upper phase; e) adding to the predetermined volume of the upper phase an amount of a mixture of an aqueous protein-precipitating agent without any adsorbing material, the amount of mixture being effective to cause precipitation of the lipid associated sialoprotein; f) vortexing the resulting admixture; g) centrifuging and recovering the resulting precipitate; h) washing the precipitate in a saline solution; i) centrifuging the resulting mixture; j) dissolving the precipitate in water; k) adding to the solution a hydrolysis agent; l) heating the resulting admixture; m) determining the amount of lipid associated sialoprotein present in the solution and thereby the amount present in the fluid sample.