Patent ID: 6379894
Filing Date: 2002-04-30
Classification: C07K,C12Q

Abstract:
A method for determining whether a candidate compound is capable of inhibiting binding between STAT1 and USF1 polypeptides, comprising steps:(a) providing all or a portion of the STAT1 polypeptide having the property of binding to the USF1 polypeptide; (b) providing all or a portion of the USF1 polypeptide having the property of binding to the STAT1 polypeptide; (c) bringing said polypeptides as defined in a) and b) into contact with said candidate compound under conditions which enable binding between the STAT1 and USF1 polypeptides; (d) measuring the binding between the STAT1 and USF1 polypeptides; and (e) comparing this measurement with that for binding between the STAT1 and USF1 polypeptides observed under the same experimental conditions in the absence of said candidate compound, a reduction in binding allowing the conclusion that said candidate compound is capable of inhibiting binding between the STAT1 and USF1 polypeptides.