Patent ID: 9187764
Filing Date: 2015-11-17
Classification: A01K,C07K,C12N

Abstract:
1. A method of controlling zebrafish reproduction, comprising the following steps: (A) Introducing exogenous genes: introducing recombinant genes CMV-eGFP-SV40-CMV-Gal4-SV40 and CMV-RFP-SV40-UAS-antisense dnd into fertilized eggs of zebrafish by microinjection method to prepare P0 generation of GAL4 transgenic zebrafish and UAS-antisense dnd transgenic zebrafish; The recombinant gene CMV-eGFP-SV40-CMV-Gal4-SV40 has a sequence shown in SEQ ID NO: 1; The recombinant gene CMV-RFP-SV40-UAS-antisense dnd has a sequence shown in SEQ ID NO: 2; (2) Obtaining homozygous transgenic zebrafish: zebrafish that expressing green fluorescent protein is P0 generation GAL4 transgenic zebrafish; and zebrafish that expressing red fluorescent protein is P0 generation UAS-antisense dnd transgenic zebrafish; hybridizing the two P0 transgenic zebrafish with control zebrafish, respectively, to obtain F1 generation GAL4 transgenic zebrafish and UAS-antisense dnd transgenic zebrafish; inbreeding the F1 generation GAL4 transgenic zebrafish, to obtain homozygous GAL4 transgenic zebrafish in F2 generation; and inbreeding the F1 generation UAS-antisense dnd transgenic zebrafish to obtain homozygous UAS-antisense dnd transgenic zebrafish in F2 generation; (3) Hybridizing transgenic zebrafish: hybridizing, by taking the homozygous GAL4 transgenic zebrafish and homozygous UAS-antisense dnd transgenic zebrafish as parents, the transgenic zebrafish of two lines, to produce sterilized zebrafish offspring.