Patent ID: 9150845
Filing Date: 2015-10-06
Classification: A61P,C12N,C12P,C12Y,D06M

Abstract:
1. A method for making modified small molecules comprising the following steps: (a) providing a plurality of biosynthetic enzymes capable of synthesizing or modifying the small molecules, wherein one of the enzymes is an ammonia lyase enzyme encoded by a nucleic acid having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more, or complete sequence identity to SEQ ID NO:31; (b) providing a substrate of small molecules comprising at least phenylalanine, tyrosine and/or histidine; and (c) reacting the substrate of step (b) with the enzymes under conditions that facilitate a plurality of biocatalytic reactions to generate modified small molecules by a series of biocatalytic reactions.