Patent ID: 9157123
Filing Date: 2015-10-13
Classification: A61K,A61P,C12N,C12Q,G01N

Abstract:
1. A method of categorizing a papillary thyroid cancer tissue sample obtained from a human patient comprising the steps of: a. performing quantitative real-time polymerase chain reaction (PCR) on genomic DNA obtained from the tissue sample using primers that will specifically amplify the IQ motif containing GTPase activating protein 1 (IQGAP1) gene, wherein the primers are selected from the group consisting of SEQ ID NO:9 and SEQ ID NO:17, SEQ ID NO:10 and SEQ ID NO:18, SEQ ID NO:11 and SEQ ID NO:19, SEQ ID NO:12 and SEQ ID NO:20, and SEQ ID NO:13 and SEQ ID NO:21; b. detecting an increased copy number of the IQGAP1 gene of at least 4 relative to the IQGAP1 copy number in normal thyroid tissue or relative to the copy number of a reference gene in the tissue sample; c. PCR amplifying the BRAF gene from genomic DNA obtained from the tissue sample using primers that will specifically amplify the nucleic acid sequence located at the BRAF T1796A (V600E) mutation site; d. sequencing the amplified PCR product of step (c) to detect the presence of the BRAF T1796A (V600E) mutation; and e. categorizing the thyroid tissue sample as belonging to a human patient who is likely to experience tumor recurrence based on the copy number of at least 4 detected in step (b) and the BRAF mutation T1796A (V600E) mutation detected in step (d).