Patent ID: 7005271
Filing Date: 2006-02-28
Classification: G01N

Abstract:
1. A method for determining a thrombotic or prethrombotic state, disease or risk therefor in an individual being screened for said state disease or risk, comprising: obtaining a body fluid sample from the individual, said sample suspected of comprising a member selected from the group consisting of circulating microparticles, stimulated procoagulant cells and mixtures thereof; incubating the sample with a solid phase-bound purified receptor specific for a phospholipid, under conditions to form an immobilized complex on the solid phase of the purified receptor and any said member present in said sample; washing the incubated solid phase to remove unbound components; and determining a level of prothrombinase activity of the washed immobilized complex, wherein an elevated level of prothrombinase activity determined for the immobilized complex compared with a level determined for normal body fluid samples indicates a thrombotic or prethrombotic state, disease, or elevated risk therefor in the individual.