Patent ID: 6060255
Filing Date: 2000-05-09
Classification: C07K,G01N,Y10S

Abstract:
A type IV collagen high molecular form having the following properties:(a) having a molecular weight higher than that of the 7S domain of type IV collagen;(b) having the 7S domain in its structure;(c) being recovered from a collagen solution digested by pepsin in the following steps:(1) salt precipitating said solution with sodium chloride at a concentration of at least 2 M, thereby forming a supernatant and a precipitate,(2) dissolving the precipitate obtained from step (1), to form a second solution,(3) salt precipitating said second solution with sodium chloride at a concentration no higher than 1.2 M, thereby forming a supernatant and a precipitate,(4) dissolving the precipitate obtained from step (3), to form a third solution,(5) salt precipitating said third solution with sodium chloride at a concentration no higher than that used in step (3), thereby forming a supernatant and a precipitate, and(6) collecting the supernatant of step (5).