Patent ID: 6225100
Filing Date: 2001-05-01
Classification: C12N

Abstract:
An isolated Clostridium arysulfotransferase comprising the following characteristics:a. a total molecular weight of about 320 kD;b. 4 subunits having a molecular weight of about 80 kD each;c. an optimum pH for sulfation activity at about 8.2;d. a specific activity of greater than about 15.0 units per milligram of protein;e. an ability to maintain greater than about 40% activity for about 2 weeks when stored at 4.degree. C. without being immobilized or otherwise protected; andf. an ability to use aryl sulfates selected from the group consisting of p-nitrophenylsulfate, p-acetylphenylsulfate, 4-methylumbelliferyl sulfate, estrone sulfate, p-nitrocatechol sulfate, phenolphthalein sulfate and indoxyl sulfate, as sulfate donors.