Patent ID: 8306757
Filing Date: 2012-11-06
Classification: C12Q,G01N,H01L,Y10T

Abstract:
1. A method for nucleic acid sequencing, comprising: disposing a plurality of template nucleic acids in at least 10 introducing a known nucleotide into the reaction volumes; coupling circuitry to field-effect transistors of the sensors to measure pH changes within the reaction volumes within a pre-specified pH range that is less than a maximum pH range detectable by the field-effect transistors, the measured pH change based on liberation of hydrogen ions detected by the field-effect transistors resulting from incorporation of the introduced known nucleotide into the one or more primers, and the circuitry further to generate output signals indicating the measured pH changes; and analyzing the output signals generated by the circuitry to determine at least a portion of sequences corresponding to a portion of the template nucleic acids.