Patent ID: 6586178
Filing Date: 2003-07-01
Classification: C07H,C12Q,Y02P

Abstract:
A nucleic acid probe which is a single-stranded nucleic acid complementary to a specific nucleic acid sequence and is labeled so as to give off a measurable fluorescent signal on hybridization with a nucleic acid containing the specific nucleic acid sequence, wherein the 3â€² end of the probe is modified as represented by the following formula (1): wherein R is â€”COOH, â€”CONH2, â€”(CH2)nOH, â€”CH(OH)â€”CH2OH, or â€”CH[(CH2)nâ€”NHR1]â€”CH2OH, R1 is H, a dye, or an amino protecting group, and n is an integer of at least 1,wherein the single-stranded nucleic acid is DNA having an oxygen atom retained at position 3 of the deoxyribose moiety.