Patent ID: 7037656
Filing Date: 2006-05-02
Classification: A61K,C12N,C12Q,Y02A

Abstract:
1. A ligand dependent transactivation system for screening insecticidal compounds, comprising: a) a first DNA construct having a nucleic acid molecule encoding an altered ecdysone receptor operably linked to a promoter, wherein said altered ecdysone receptor is an ecdysone receptor with a deleted A/B domain; b) a second DNA construct having a nucleic acid molecule encoding a receptor which heterodimerizes with said ecdysone receptor upon transactivation, said nucleic acid molecule being operably linked to a promoter, wherein said receptor is selected from the group consisting of insect ultraspiracle gene product (USP) receptors and mammalian retinoid X receptors (RXR); c) a third DNA construct comprising a promoter containing a plurality of ecdysone response elements, said promoter being operably linked to a reporter gene; d) a fourth DNA construct encoding a co-activator molecule, said co-activator molecule being operably linked to a promoter sequence; and e) a host cell comprising said first, second, third and fourth DNA constructs, expression of said reporter gene being dependent upon ligand dependent transactivation effectuated by said insecticidal compound.