Patent ID: 6562959
Filing Date: 2003-05-13
Classification: C12Q,G01N

Abstract:
A set of nucleic acid probes for labeling one or more chromosomes, said probes comprising a mixture of two interspersed repeated sequence-polymerase chain reaction (IRS-PCR) amplification products from said chromosome(s), wherein:said first amplification product is complementary to either a specific primer for Alu DNA sequences or a specific primer for long interspersed element (LINE) sequences; said second amplification product is complementay to a specific primer for Alu DNA sequences and a specific primer for LINE DNA sequences; and said nucleic acid probes are directly or indirectly labeled with: a) a fluorophore having an absorption bandwidth wavelength interval spanning 593 nm and an emission bandwidth wavelength interval spanning 613 nm b) a fluorophore having an absorption bandwidth wavelength interval spanning 554 nm and an emission bandwidth wavelength interval spanning 568 nm; and c) up to five fluorophores selected from the group consisting of: i) a fluorophore having an absorption bandwidth wavelength interval spanning 494 nm and an emission bandwidth wavelength interval spanning 517 nm; ii) a fluorophore having an absorption bandwidth wavelength interval spanning 652 nm and an emission bandwidth wavelength interval spanning 670 nm; iii) a fluorophore having an absorption bandwidth wavelength interval spanning 675 nm and an emission bandwidth wavelength interval spanning 694 nm; iv) a fluorophore having an absorption bandwidth wavelength interval spanning 743 nm and an emission bandwidth wavelength interval spanning 767 nm; v) and a fluorophore having an absorption bandwidth wavelength interval spanning 632 nm and an emission bandwidth wavelength interval spanning 658 nm; and wherein the overlapping of the absorption and emission bandwidth wavelength intervals between the different fluorophores is minimal and there is no overlapping between the absorption and emission maxima of the different fluorophores.