Patent ID: 8048681
Filing Date: 2011-11-01
Classification: C07H,C12N,Y10T

Abstract:
1. A method for nucleic acid isolation, comprising the steps of: mixing a chaotropic agent with a sample containing nucleic acids; allowing the mixed solution to pass at least twice through a first solid phase containing silica that has passage pores having predetermined pore sizes; allowing the mixed solution passed through the first solid phase to pass at least twice through a second solid phase containing silica that has passage pores having pore sizes smaller than those of the first solid phase containing silica; wherein passage pores of the first solid phase containing silica have pore sizes of 20 to 25 μm and passage pores of the second solid phase containing silica have pore sizes of 0.1 to 10 μm, thereby, separating and capturing nucleic acids according to the base sequence lengths by the action of the first and second solid phases with the different pore sizes; and separately recovering nucleic acids that have bound to the first solid phase containing silica and those that have bound to the second solid phase containing silica.