Patent ID: 6410274
Filing Date: 2002-06-25
Classification: C12N

Abstract:
A process for purification of plasmid DNA by separation on an insoluble matrix comprising the following steps:a) lysing cells containing said plasmid DNA to obtain a lysate comprising plasmid DNA, chromosomal DNA and RNA; b) precipitating most of the RNA and most of the chromosomal DNA in the lysate by adding divalent alkaline earth metal ions to the lysate from step a); c) removing the precipitated RNA and chromosomal DNA from the lysate from step b); d) contacting the lysate from step c) with an anion exchange matrix containing anion exchange groups to obtain purified plasmid DNA; and e) contacting the purified plasmid DNA from step d) with an anion exchange matrix different from the anion exchange matrix of step d) to obtain purified plasmid DNA of gene therapy grade.