Patent ID: 8790928
Filing Date: 2014-07-29
Classification: C12N

Abstract:
1. A method of amplifying gene expression in a moss plant cell by increasing the copy number of integrated transforming DNA constructs, the method comprising: 1) providing at least a first heterologous nucleic acid construct comprising at least one heterologous nucleotide sequence operably linked to a promoter, wherein said construct is flanked at the 5′ end thereof by a first recombination sequence and is flanked at the 3′ end of said construct by a second recombination sequence; 2) providing at least a second heterologous nucleic acid construct different from the first, said second construct comprising at least one heterologous nucleotide sequence operably linked to a promoter, wherein said construct is flanked at the 5′ end thereof by said second recombination sequence and is flanked at the 3′ end of said construct by said first recombination sequence; 3) transforming into the moss plant cell at least said first and said second heterologous nucleic acid constructs; and 4) regenerating the transformed moss plant cell into moss protonema comprising a plurality of copies of said at least one heterologous nucleotide sequence;