Patent ID: 6156497
Filing Date: 2000-12-05
Classification: C12N

Abstract:
A hybrid adenoviral-retroviral vector including:an adenoviral 5' ITR;an adenoviral packaging signal;a retroviral 5' LTR;a retroviral packaging signal;a DNA sequence encoding a protein heterologous to adenovirus and retrovirus;a retroviral 3' LTR;a DNA sequence encoding a retroviral gag protein and a retroviral pol protein;a DNA sequence encoding a retroviral env protein; andan adenoviral 3' ITR, wherein (i) said adenoviral 5' ITR and said adenoviral 3' ITR flank said adenoviral packaging signal, said retroviral 5' LTR, said retroviral packaging signal, said DNA sequence encoding said protein heterologous to adenovirus and retrovirus, said retroviral 3' LTR, said DNA sequence encoding a retroviral gag protein and a retroviral pol protein, and said DNA sequence encoding a retroviral env protein; (ii) said retroviral 5' LTR and said retroviral 3' LTR flank said retroviral packaging signal and said DNA encoding a protein heterologous to adenovirus and retrovirus; (iii) said DNA sequence encoding a retroviral gag protein and a retroviral pol protein and said DNA sequence encoding the retroviral env protein are not flanked by said retroviral 5' LTR and said retroviral 3' LTR; and (iv) said adenoviral packaging signal is not flanked by said retroviral 5' LTR and said retroviral 3' LTR and is located sufficiently proximal to one of said adenoviral 5' ITR or said adenoviral 3' ITR to enable said hybrid adenoviral-retroviral vector to be packaged.