Patent ID: 7544494
Filing Date: 2009-06-09
Classification: C12P

Abstract:
1. A process for producing vitamin C from L-sorbosone which comprises contacting L-sorbosone with a purified L-sorbosone dehydrogenase having the following physico-chemical properties: a) Molecular weight: 150,000±6,000 Da or 230,000±9,000 Da (consisting of 2 or 3 homologous subunits, each subunit having a molecular weight of 75,000±3,000 Da) b) Substrate specificity: active on aldehyde compounds c) Cofactors: pyrroloquinoline quinone and heme c d) Optimum pH: 6.4 to 8.2 for the production of vitamin C from L-sorbosone e) Inhibitors: Co