Patent ID: 6518051
Filing Date: 2003-02-11
Classification: C12N,C12P

Abstract:
A method for synthesizing a saccharide composition, the method comprising:providing a glycoprotein immobilized on a solid support; contacting the glycoprotein with (a) a donor moiety that comprises a pre-selected saccharide unit, wherein the donor moiety is selected from the group consisting of CMP-NeuAc, UDP-Gal, GDP-Fuc, UDP-GalNAc and UDP-GlcNAc, and with (b) a glycosyltransferase that catalyzes the transfer of the pre-selected saccharide unit to the glycoprotein, wherein the glycosyltransferase is selected from the group consisting of a sialyltransferase, a galactosyltransferase, a fucosyltransferase, an N-acetylgalactosaminyltransferase, and an N-acetylglucosaminyltransferase; wherein transfer of the pre-selected saccharide unit from the donor moiety to the glycoprotein synthesizes the saccharide composition.