Patent ID: 7998747
Filing Date: 2011-08-16
Classification: G01N,Y10T

Abstract:
1. A method of determining the dilution achieved in carrying out a dilution protocol using a sample solution and a diluent, wherein the sample solution includes a first chromophore that absorbs light at a first wavelength and a known concentration of a second chromophore that absorbs light at a second wavelength, the method comprising the steps of: a. adding to a first vessel of a first set of a plurality of vessels a target volume of a first sample solution, wherein the first sample solution includes a first known concentration of the first chromophore and a known concentration of the second chromophore, wherein each of the first chromophore and the second chromophore has an absorbance maximum, the absorbance maximum of the first chromophore minimally overlapping with the absorbance maximum of the second chromophore; b. adding to a first vessel of a second set of the plurality of vessels a target volume of a second sample solution including a second known concentration of the first chromophore and the known concentration of the second chromophore, wherein the second known concentration of the first chromophore of the second sample solution is higher than the first known concentration of the first chromophore of the first sample solution, and wherein the target volume of the second sample solution added to the first vessel of the second set is substantially equivalent to the target volume of the first sample solution added to the first vessel of the first set; c. carrying out a first dilution protocol step comprising: d. carrying out a second dilution protocol step comprising: e. calculating a dilution ratio of the dilution protocol based on the absorbance measurements made in steps c.iii and d.v, wherein the dilution ratio represents the extent of dilution occurring between the first dilution protocol step and the second dilution protocol step.