Patent ID: 8008465
Filing Date: 2011-08-30
Classification: A61K,C07K,Y02A,Y10S

Abstract:
1. A nucleic acid comprising a region that encodes a modified toxin comprising a modified light chain of a botulinum toxin type E, wherein the modification to the botulinum toxin type E light chain is the addition of one or more amino acid sequences comprising SEQ ID NO: 120 within the N-terminal 30 amino acids of a wild-type botulinum toxin type E light chain of SEQ ID NO: 136 and the addition of one or more leucine-based motifs of SEQ ID NO: 112 within the C-terminal 50 amino acids of a wild-type botulinum toxin type E light chain of SEQ ID NO: 136, wherein the addition of the amino acid sequence comprising SEQ ID NO: 120 increases biological half-life of the modified toxin relative to an identical modified toxin without the additional amino acid sequence comprising SEQ ID NO: 120, and, wherein the addition of the leucine-based motif of SEQ ID NO: 112 increases biological half-life of the modified toxin relative to an identical modified toxin without the additional leucine-based motif comprising SEQ ID NO: 112.