Patent ID: 6684162
Filing Date: 2004-01-27
Classification: C12N,G16B

Abstract:
A method for identifying an agent that interacts with an active site of an acyl carrier protein synthase-acyl carrier protein (ACPS-ACP) complex, comprising the steps of:(a) obtaining a crystallized complex comprising ACPS-ACP, wherein the crystallized complex is characterized as being in rod-shape form with space group C2221, and having unit cell parameters of a=78.46 Ã¥, b=122.03 Ã¥ and c=136.77 Ã¥; (b) obtaining the structural coordinates of amino acids of the crystallized complex of step (a), wherein the structural coordinates are set forth in FIGS. 3 and 3A-1 to 3A-79; (c) generating a three dimensional model of ACPS-ACP using the structural coordinates of the amino acids generated in step (b), Â±a root mean square deviation from the backbone atoms of said amino acids of not more than 1.5 Ã¥; (d) determining an active site of the ACPS-ACP complex from said three dimensional model; and (e) performing computer fitting analysis to identify an agent which interacts with said active site.