Patent ID: 6653129
Filing Date: 2003-11-25
Classification: G01N

Abstract:
A method for isolating viable epithelial cells from a solution, the method comprising:providing an atibody or antigen-binding portion thereof which binds to an extracellular domain of prostate specific membrane antigen (PSMA); contacting said antibody or antigen-binding portion thereof with a magnetizable medium under conditions permitting binding of said antibody or antigen-binding portion thereof to said magnetizable medium; contacting a solution containing said epithelial cells with said magnetizable medium bound to said antibody or antigen-binding portion thereof under conditions permitting binding of said antibody or antigen-binding portion thereof to said epithelial cells to form a complex including said magnetizable medium, said antibody or antigen-binding portion thereof, and said epithelial cells; contacting said complex with a magnetized matrix under conditions permitting isolation of said complex from said solution; and eluting said epithelial cells from said magnetized matrix.