Patent ID: 8668872
Filing Date: 2014-03-11
Classification: G01N,H04N,Y10S,Y10T

Abstract:
1. A device for imaging a dyed object comprising: at least one objective lens; a sample holder located adjacent to the at least one objective lens; an excitement component in combination with the sample holder, wherein the excitement component is adapted to excites fluorescence of the dyed object; at least one camera in combination with the at least one objective lens to detect fluorescence light of the dyed object through the at least one objective lens; and a recordable medium in combination with the at least one camera, and adapted to record at least one signal based on the fluorescence light detected by the at least one camera; wherein the device is adapted to excite fluorescent molecules within the dyed object and/or cause or allow at least some of the fluorescent molecules to lose color, such that a mean distance between a plurality of the fluorescent molecules allows detection by the at least one camera of the plurality of the fluorescent molecules as separate spots; and wherein the device is adapted to repeat cycles of exciting fluorescence of the dyed object, detecting fluorescence light of the dyed object, recording the fluorescence light detected by the at least one camera, and causing or allowing at least some of the fluorescent molecules to lose color.