Patent ID: 6794153
Filing Date: 2004-09-21
Classification: C07K,G01N,Y10S

Abstract:
A method for detecting a Helicobacter pylori antigen in a serum sample obtained from a human comprising:providing said serum sample from said human; wherein said human has symptoms of peptic ulcer disease and/or chronic gastritis; treating said serum sample with a dissociation reagent; removing said dissociation reagent from said serum to form a dissociation reagent-removed serum sample; providing a first antibody against Helicobacter pylori, wherein said first antibody is a lyclonal antibody purified by an affinity column with potassium thiocynate (KSCN) in water; contacting said dissociation reagent-treated serum sample with the first antibody to form a first complex; providing a second antibody against Helicobacter pylori, wherein said second antibody is a polyclonal antibody purified by an affinity column with potassium thiocynate (KSCN) in water; wherein said first antibody and said second antibody are eluted from said affinity column with potassium thiocynate (KSCN) in water; wherein one of said first and second antibody is bound to a solid carrier, the other is labeled with a detection agent; contacting said first complex with the second antibody to form a second complex; and detecting the presence of said Helicobacter pylori antigen by measuring the presence of the detection agent in said second complex.