Patent ID: 9006414
Filing Date: 2015-04-14
Classification: A61K,C07H,C12N

Abstract:
1. A recombinant DNA construct for suppression of at least one target gene which comprises in 5′ to 3′ order a promoter element operably linked to an anti-sense-oriented DNA element from at least one target gene and a sense-oriented DNA element comprising at least 21 nucleotides, wherein the sense-oriented DNA element is not more than about one-half the length of the anti-sense-oriented DNA element, and sense-oriented RNA transcribed from the sense-oriented DNA is complementary to and forms a double-stranded segment with the 5′-most end of anti-sense-oriented RNA transcribed from the anti-sense-oriented DNA element, wherein said transcribed RNA forms said double-stranded segment and an adjacent loop of anti-sense-oriented RNA for suppressing said at least one target gene.