Patent ID: 6593106
Filing Date: 2003-07-15
Classification: C07K

Abstract:
A method of expressing a protein from the transforming growth factor-beta (TGF-&bgr;) superfamily comprising:a) culturing prokaryotic cells which have been transformed with a DNA encoding a protein from the TGF-&bgr; super family under conditions suitable for the production of protein; and b) refolding said protein in refolding media comprising an amide substituted pyrrole compound or a compound selected from the group consisting of pyrrole 3-sulfonic acid, pyrrole-2 carboxylic acid, 3-pyrrole acetic acid hydrochloride, 2-pyrrole acetic acid hydrochloride, 2-pyrrole ethane sulfonic acid, and 3-pyrrole hydroxymethane sulfonic acid.