Patent ID: 6803238
Filing Date: 2004-10-12
Classification: B01J,B01L,C40B,G01N,Y10S,Y10T

Abstract:
A method for detecting a labeled sample molecule bound to an immobilized probe by electrochemiluminescence using an array comprising an embedded conductive material comprising:(a) providing a multiplexed molecular analysis system comprising a reaction chamber comprising a reaction substrate comprising a plurality of biosites, each biosite comprising a plurality of similar capture probes attached to the reaction substrate, the reaction substrate comprising an embedded conductive material which is coupled to one or more biosites connected to an electrical source; (b) providing a sample comprising a plurality of labeled sample molecules; (c) adding a solution comprising the sample of step (b) to the reaction chamber of step (a); (d) applying a voltage from the electrical source to the embedded conductive material after sufficient time has passed for the labeled sample molecule to bind to a capture probe, thereby inducing an electrochemical event within an electrochemiluminescent label that releases a detectable amount of electromagnetic energy; and, (e) detecting the released electromagnetic energy indicating a bound labeled sample molecule.