Patent ID: 6258599
Filing Date: 2001-07-10
Classification: A61K,A61P,C07K,C12N

Abstract:
A method for obtaining antibodies which react with an epitope on a neutralizing or inactivating region of an HIV protein, wherein said neutralizing or inactivating region of said protein fails to elicit an immune response in man when encountered by infection or environmental exposure but does elicit an immune response in a non-human mammal which comprises:(a) isolating proteins from a lysate of an HIV isolate;(b) identifying an epitope on at least one of said proteins which has an amino acid sequence which corresponds to or mimics the amino acid sequence of a neutralizing or inactivating region which fails to elicit an immune response in man when encountered by infection or environmental exposure but does elicit an immune response in a non-human mammal;(c) combining said proteins with a physiologically acceptable carrier;(d) immunizing a non-human mammalian host with said proteins and carrier; and(e) obtaining antibodies to said epitope from said immunized host.