Patent ID: 7524681
Filing Date: 2009-04-28
Classification: G01N,Y10S,Y10T

Abstract:
1. Method for determining the condition of a biological fluid comprising the steps of (a) providing several native samples of the biological fluid, the condition of the biological fluid in each sample being known; (b) recording the IR spectra of the native samples of step (a); (c) subjecting the IR spectra of step (b) to a multiparameter analytical procedure and selecting the classification parameters that will ensure a reliable assignment of the samples to the known conditions; (d) storing the classification parameters obtained in step (c); (e) providing a native sample of a biological fluid, the condition of which is unknown; (f) recording at least one IR spectrum of the native sample of step (e); (g) subjecting the IR spectrum of step (f) to a multiparameter analysis; and (h) comparing the classification parameters of the IR spectrum of the unknown sample with the classification parameters of the IR spectra of the known samples stored in step (d), wherein the IR spectra in steps (b) and (f) are recorded under non-flowing and stopped conditions after filling the flow-through measuring cell with said sample or with a transport medium for a corresponding reference measurement, wherein the measuring cell has a path length of not more than 30 μm and a path length deviation of less than 1 nm between any two consecutive measurements requiring filling or rinsing of said measuring cell between said consecutive measurements, which filling or rinsing can cause flow- or pressure-induced path length deviations.