Patent ID: 7727716
Filing Date: 2010-06-01
Classification: C07D,C09B,C12Q,G01N,Y10T

Abstract:
1. A method for determining the presence or absence of nucleic acid in a sample, wherein the method comprises: a) combining an unsymmetrical cyanine dye compound with a sample to prepare a labeling mixture, wherein the sample is immobilized on a solid or semi-solid support and the unsymmetrical cyanine dye compound has the formula b) incubating the labeling mixture for a sufficient amount of time for the unsymmetrical cyanine dye compound to associate with the nucleic acid to prepare an incubated sample; c) illuminating the incubated sample with an appropriate wavelength to prepare an illuminated sample; and d) observing the illuminated sample whereby the presence or absence of the nucleic acid is determined.