Patent ID: 8999730
Filing Date: 2015-04-07
Classification: G01N

Abstract:
1. An immunochromatography method, which comprises: spreading a test substance, a first labeling substance modified with a first binding substance that binds to the test substance, and a second labeling substance modified with the first binding substance that binds to the test substance, which are in a mixed state, on an insoluble carrier; capturing the test substance, the first labeling substance, and/or the second labeling substance at a reaction site on the insoluble carrier comprising an immobilizing reagent capable of binding to the test substance, so as to detect the test substance, measuring the second labeling substance at the reaction site on the insoluble carrier; and thereafter amplifying the first labeling substance at the reaction site on the insoluble carrier; and thereafter measuring the first labeling substance at the reaction site on the insoluble carrier, wherein the first labeling substance is a labeling substance that causes an amplification reaction, and the second labeling substance is a labeling substance that does not substantially influence on the amplification reaction, wherein the first labeling substance is a metal colloid and the second labeling substance is a colored particle, and wherein the first labeling substance is a substance that reacts with silver ions and a reducing agent used for such silver ions to generate metallic silver, and the second labeling substance is a substance that does not substantially have a reactivity with silver ions and a reducing agent used for the silver ions, and wherein the number of metal colloid particles which are applied to the insoluble carrier is from 1×10