Patent ID: 6059561
Filing Date: 2000-05-09
Classification: C12Q,G01N,Y10S

Abstract:
A method for determining the presence or amount of an analyte in a sample consisting essentially of:a) providing to said sample a labeled probe under conditions such that said labeled probe preferentially binds to said analyte in the presence of non-analyte, thereby forming a probe:analyte complex stable for detection;b) providing to said sample one or more amphiphiles which can alter the detectability of said labeled probe which does not form part of a probe:analyte complex, so that the detectability of said labeled probe which forms part of a probe:analyte complex is distinguishable from the detectability of said labeled probe which does not form part of a probe:analyte complex in the presence of said amphiphiles, wherein a charge of said probe and the charge of at least one of said amphiphiles are opposite charges; andc) detecting said labeled probe which forms part of a probe:analyte complex as an indication of the presence or amount of said analyte in said sample.