Patent ID: 8865401
Filing Date: 2014-10-21
Classification: C07K,G01N,Y10S

Abstract:
1. A method of simultaneously separating and concentrating nucleic acid and protein targets, the steps comprising: (i) obtaining a sample comprising a nucleic acid and a protein; (ii) adding said sample to a gel electrophoresis unit, said gel electrophoresis includes a gel box with a negative electrode side and a positive electrode side, the negative electrode side being filled with a first buffer comprising 2-Hydroxy-N-(tris(hydroxymethyl) methyl)-3-aminopropanesulfonic acid buffer and the positive electrode side being filled with a second buffer being different than said first buffer; (iii) subsequent to step (ii), subjecting said sample to isotachophoresis, said subjecting step comprising a step of applying a voltage to said gel electrophoresis unit; and (iii) subsequent to step (iii), detecting the presence of the nucleic acid and the protein.