Patent ID: 9222080
Filing Date: 2015-12-29
Classification: C12N,C12P

Abstract:
1. A method of coupling an agent to a carrier protein comprising: incubating a reaction mixture comprising a bioactive agent, a carrier protein and a polypeptide fragment of a mutated alpha 1,3 N-acetylgalactosaminyltransferase (alpha 3GalNAc T) that is a mutated form of a 1,3 galactosyltransferase (alpha 3 Gal-T) comprising one or more mutations in the sugar donor binding site or hinge region of the alpha 3 Gal-T and that retains the ability to transfer a donor sugar with a chemically reactive functional group to a sugar acceptor, wherein said sugar acceptor is attached to the carrier protein and wherein said donor sugar is transferred to said sugar acceptor and said transferred donor sugar is then coupled to the bioactive agent having a group that reacts with said chemically reactive functional group, thereby forming an oligosaccharide that couples said bioactive agent to said carrier protein.