Patent ID: 6258566
Filing Date: 2001-07-10
Classification: A01K,C07K,C12N,C12P

Abstract:
A modified recombinant host cell, which, in unmodified form does not produce polyketides, which cell is modified to contain an expression system for a minimal polyketide synthase (PKS) and an expression system for a holo acyl carrier protein (ACP) synthase,wherein said minimal PKS comprises a ketosynthase (KS) domain, an acyl transferase (AT) domain, and an acyl carrier protein (ACP) domain for a modular PKS or a fungal PKS; andwherein said holo ACP synthase is selected from the group consisting of:the enterobactin synthetase holo-ACP synthase (EntD) from Escherichia coli,the gramacidin synthetase holo-ACP synthase (GsP) from Bacillius brevis, andthe surfactin synthetase holo-ACP synthase (sfp) from Bacillius subtilis.