Patent ID: 6214221
Filing Date: 2001-04-10
Classification: B01D,C07K,C12M

Abstract:
A system for isolating one or more target substances from a source liquid, comprising:(a) a first reservoir comprising:(i) input means for selectively flowing liquid into the first reservoir; and(ii) output means for selectively flowing liquid out of the first reservoir;(b) a first cross-flow filtration module for separating liquids into permeate and retentate streams, the module comprising:(i) liquid input means coupled via a first flow circuit in fluid communication with the output means of the first reservoir for flowing liquid from the first reservoir into the first cross-flow filtration module;(ii) permeate liquid output means for flowing permeate out of the first cross-flow filtration module; and(iii) retentate liquid output means for flowing retentate out of the first cross-flow filtration module; and(c) chromatography resin(s) having affinity for one or more target substances, the chromatography resin:(i) situated in the first reservoir and/or in a flow circuit upstream of the first cross-flow filtration module, wherein the first reservoir and/or in a flow circuit upstream of the first cross-flow filtration module further comprises a means for bringing the source liquid into association with the chromatography resin; and/or(ii) situated in a contact zone for contacting chromatography resin and source liquid, said contact zone comprising a reservoir and/or flow circuit and optionally comprising a mixing device upstream of the first cross-flow filtration module.