Patent ID: 6709819
Filing Date: 2004-03-23
Classification: C12Q

Abstract:
A method for identifying the presence of a nucleic acid target in a sample by determination of structure formation with said nucleic acid target, comprising the steps of:a) providing: i) a sample suspected of having a folded target having a deoxyribonucleic acid sequence comprising one or more double stranded regions, and one or more single stranded regions, and further comprising two or more non-contiguous portions, and one or more intervening regions; and ii) one or more bridging oligonucleotide probes complementary to said two or more non-contiguous portions of said folded target; and b) mixing said folded target and said one or more probes under conditions such that said probe hybridizes to said folded target to form a probe/folded target complex; and c) detecting said probe/folded target complex, thereby detecting the presence of said folded target in said sample.