Patent ID: 6319675
Filing Date: 2001-11-20
Classification: A61K,C07K,Y10S

Abstract:
A method of detecting and/or identifying an agent which binds to Bonzo or a SExCkine-binding variant of Bonzo comprising combining:a) SExCkine or a Bonzo-binding variant of SExCkine,b) a test agent, andc) a composition comprising Bonzo or a SExCkine-binding variant of Bonzo under conditions suitable for binding of said SExCkine or Bonzo-binding variant to said Bonzo or SExCkine-binding variant; anddetecting or measuring the formation of a complex between said SExCkine or Bonzo-binding variant and said Bonzo or SExCkine-binding variant, wherein a decrease in the formation of said complex relative to a suitable control indicates that said test agent binds to said Bonzo or SExCkine-binding variant,wherein said SExCkine or Bonzo-binding variant is selected from the group consisting of (i) SEQ ID NO:4, (ii) SEQ ID NO:6, (iii) a Bonzo-binding fragment of SEQ ID NO:4, (iv) a Bonzo-binding fragment of SEQ ID NO:6 and (v) a Bonzo-binding polypeptide having 90% amino acid sequence similarity over the full length of said Bonzo-binding polypeptide to any one or (i)-(iv); andwherein said Bonzo or SExCkine-binding variant is selected from the group consisting of (1) SEQ ID NO:2, (2) a SExCkine-binding fragment of SEQ ID NO:2 and (3) a SExCkine-binding polypeptide having 90% amino acid sequence similarity over the full length of said SExCkine-binding polypeptide to (1) or (2).