Patent ID: 6441140
Filing Date: 2002-08-27
Classification: C07K

Abstract:
A method for producing a motif-specific, context-independent antibody that recognizes a plurality of peptides or proteins within a genome that contain the motif, said method comprising the steps of:(a) constructing a combinatorial peptide library comprising (i) a fixed motif, comprises a kinase consensus substrate motif or a protein-protein binding motif and wherein said fixed motif comprises at least one phosphorylated amino acid selected from the group consisting of phosphothreonine, phosphoserine, and phosphotyrosine, and (ii) a plurality of degenerate amino acids surrounding said motif; (b) immunizing a host with said peptide library; and (c) isolating antisera from said host, and purifying said motif-specific, context-independent antibody from said antisera, said antibody recognizing a plurality of peptides or a proteins with a genome that contain said motif.