Patent ID: 6420131
Filing Date: 2002-07-16
Classification: C07D,C12Q,G01N,Y10S

Abstract:
A method for assaying cytochrome P450 enzyme activity comprising:contacting a cytochrome P450 enzyme with a compound selected from the group consisting of: (a) a compound of Formula I; (1) wherein R1 is aryl and R2 is independently selected from the group consisting of an hydrido and an aryl, provided that R1 and R2 are not both phenyl when n and m are 1; and wherein the aryl contains an aryl ring carbon and/or an aryl ring nitrogen and the (CH2)n or (CH2)m is coupled via a covalent bond to the aryl ring carbon or the aryl ring nitrogen; (2) wherein n is 0, 1, 2, or 3; (3) wherein X1, X2, X3, X4, X5, and X6 are independently selected from the group consisting of an hydrido, a chloro, a fluoro, a bromo, and an iodo; and (4) wherein m is 0, 1, 2, or 3; and (5) wherein the compound is a cytochrome P450 substrate; (b) a compound of Formula III; and (1) wherein R1 and R2 are each phenyl; and wherein the phenyl contains an aryl ring carbon and the (CH2)n or (CH2)m is coupled via a covalent bond to the aryl ring carbon; (2) wherein n is 1; (3) wherein X1, X2, X3, X4, X5, and X6 are independently selected from the group consisting of a chloro, a fluoro, a bromo, and an iodo; (4) wherein m is 1; and (5) wherein the compound is a cytochrome P450 substrate; and (c) benzyloxyfluorescein benzyl ester (DBF); under conditions whereby the cytochrome P450 enzyme catalyzes the conversion of the compound to a fluorescent product.