Patent ID: 8435775
Filing Date: 2013-05-07
Classification: C12N,C12P,C12Q

Abstract:
1. A purified engineered DNA polymerase comprising an amino acid sequence, wherein the engineered polymerase has an expanded substrate range and incorporates an enhanced occurrence of a dye-labelled nucleotide analogue into nucleic acid synthesized by the engineered polymerase as compared with the wild type polymerase from which it is derived, wherein the engineered DNA polymerase has at least 90% identity to SEQ ID NO: 1, 4, 8, 12, 14, 16, 20, 22, 24, 26, 28, 30, 32, 34, 36, 38, 40, 42, 44, 46 or 48, and wherein the engineered DNA polymerase comprises the following substitution mutations compared with the Pyrococcus furiosus (Pfu) wild-type DNA polymerase amino acid sequence set forth in SEQ ID NO: 134: N400G/N400D and R407I.