Patent ID: 8206952
Filing Date: 2012-06-26
Classification: C12P,C12Q

Abstract:
1. A method for synthesizing a nucleic acid molecule, the method comprising: a) providing a first immobilized nucleic acid comprising a first 5′ region and a first 3′ region; b) providing a second immobilized nucleic acid comprising a second 5′ region and a second 3′ region, wherein said second 3′ region and said first 5′ region comprise identical nucleic acid sequences; c) hybridizing said first immobilized nucleic acid with an oligonucleotide under conditions promoting hybridization of said oligonucleotide to said first 3′ region, extending the hybridized oligonucleotide, and producing a first extension product comprising a 3′ region that is complementary to said first 5′ region; and d) hybridizing said second immobilized nucleic acid with said first extension product under conditions promoting hybridization of said 3′ region of said first extension product to said second 3′ region, extending the 3′ region of said first extension product, and producing a second extension product comprising a 3′ region that is complementary to said second 5′ region, wherein said second extension product comprises a sequence complementary to said first 3′ and 5′ regions and said second 3′ and 5′ regions and said second extension product comprises the synthesized nucleic acid molecule.