Patent ID: 6703244
Filing Date: 2004-03-09
Classification: G01N,Y10T

Abstract:
A method for confirming the presence of trichothecene mycotoxins in a person comprising the steps of:a. collecting a sample of urine from a person; b. extracting the urine using a predetermined ratio by volume of urine and ethyl acetate; c. separating the extracted urine into an aqueous portion of the urine and an ethyl acetate portion, and discarding the aqueous portion of the urine; d. drying the ethyl acetate portion to produce a residue; e. rinsing and drying the residue; f. adding ethyl alcohol to the residue; g. subjecting the ethyl alcohol and the residue to thin layer chromatography or gas chromatography; and h. observing the ethyl alcohol and the residue subjected to the thin layer chromatography or gas chromatography for the presence of mycotoxins and thereby confirming or denying exposure of the person to mycotoxins.