Patent ID: 8003375
Filing Date: 2011-08-23
Classification: C12Q

Abstract:
1. A device for identifying the presence or absence of at least a first differentially spliced product of a first gene or RNA, wherein said device comprises a solid support material and single-stranded oligonucleotides of between 5 and 100 nucleotides in length attached to said support material, wherein at least one of said oligonucleotides has a length of 25-100 nucleotides, wherein said oligonucleotides comprise at least a first and a second oligonucleotide molecule arranged serially on the support material, wherein said first oligonucleotide molecule comprises a first sequence that is complementary to and specific for a first exon or exon-exon or exon-intron junction region of said first gene or RNA, and wherein said first sequence corresponds to a region of variability in at least one product of said first gene or RNA due to differential splicing, and wherein said second oligonucleotide molecule comprises a second sequence that is complementary to and specific for a second exon or exon-exon or exon-intron junction region of said first gene or RNA, and wherein said second sequence corresponds to a region of variability in at least one product of said first gene or RNA due to differential splicing, said device allowing, when contacted with a diverse population of nucleic acid molecules prepared from a biological sample under conditions allowing hybridisation to occur, the determination of the presence or absence of said first differentially spliced product relative to at least a second differentially spliced product of said first gene or RNA.