Patent ID: 7799525
Filing Date: 2010-09-21
Classification: C12Q

Abstract:
1. A method for whole genome amplification comprising: (a) treating genomic DNA with a bisulphite reagent to form treated genomic DNA, removing the bisulphite reagent and incubating the treated genomic DNA at pH of between 10 and less than 12.5 to form substantially unfragmented single stranded modified DNA, wherein cytosine bases but not 5′-methyl-cytosine bases in the genomic DNA are modified to uracil bases to form the modified DNA; (b) providing a population of random X-mers of exonuclease-resistant primers capable of binding to at least one strand of the modified DNA, wherein X is an integer 3 or greater, and wherein the primers are formed of two populations of primers, the first population comprising random X-mers containing only the bases A, G and T, and the second population comprising random X-mers containing only the bases A, C and T; (c) contacting the modified DNA and the population of random X-mers with nucleotides and a polymerase capable of amplifying double stranded DNA; and (d) allowing the polymerase to amplify the modified DNA.