Patent ID: 9091651
Filing Date: 2015-07-28
Classification: C07K,G01N

Abstract:
1. A composition comprising at least 5 transition ion pairs said composition comprising at least one transition ion pair of at least 5 proteins selected from LRP1, BGH3, COIA1, TETN, TSP1, ALDOA, GRP78, ISLR, FRIL, LG3BP, PRDX1, FIBA, or GSLG1, wherein the at least one transition ion pair consists of a precursor ion with a corresponding m/z and a fragment ion with a corresponding m/z, and wherein the transition ion pairs are selected from precursor ADDGRPFPQVIK (SEQ ID NO: 57) transition pair 671.86-260.2, 671.86-584.4, or 671.86-528.5; precursor ALQASALK (SEQ ID NO: 58) transition pair 401.25-260.2, 401.25-331.2, 401.25-489.3, or 401.25-617.4; precursor QLLLTADDR (SEQ ID NO: 60) transition pair 522.79-355.2, 522.79-577.3, 522.79-690.3, or 522.79-803.4; precursor ADHHATNGVVHLIDK (SEQ ID NO: 136) transition pair 542.95-262.1, 542.95-488.3, 542.95-625.4, or 542.95-724.4; precursor DILATNGVIHYIDELLIPDSAK (SEQ ID NO: 137) transition pair 804.1-305.2, 804.1-514.3, 804.1-517.3, or 804.1-630.3; precursor LTLLAPLNSVFK (SEQ ID NO: 139) transition pair 658.4-328.2, 658.4-804.5, 658.4-875.5, or 658.4-988.6; precursor SPYQLVLQHSR (SEQ ID NO: 140) transition pair 443.24-527.3, 443.24-589.3, 443.24-640.4, or 443.24-852.5; precursor ADDILASPPR (SEQ ID NO: 331) transition pair 527.78-302.1, 527.78-369.2, 527.78-369.2, 527.78-456.3, or 527.78-640.4; precursor AVGLAGTFR (SEQ ID NO: 332) transition pair 446.26-480.3, 446.26-551.3, 446.26-664.4, or 446.26-721.4; precursor TEAPSATGQASSLLGGR (SEQ ID NO: 335) transition pair 801.91-289.2, 801.91-302.1, 801.91-760.4, or 801.91-945.5; precursor GGSTSYGTGSETESPR (SEQ ID NO 554) transition pair 786.84-359.2, 786.84-589.3, 786.84-862.4, or 786.84-963.4; precursor NSLFEYQK (SEQ ID NO: 556) transition pair 514.76-315.2, 514.76-567.3, 514.76-714.3, or 514.76-827.4, precursor TVIGPDGHK (SEQ ID NO: 557) transition pair 308.5-341.2, 308.5-456.2, 308.5-553.3, or 308.5-610.3; precursor VQHIQLLQK (SEQ ID NO: 558) transition pair 369.56-501.3, 369.56-606.3, 369.56-629.4, or 369.56-742.5; precursor ALFQDIK (SEQ ID NO: 593) transition pair 417.74-260.2, 417.74-503.3, 417.74-650.4, or 417.74-763.4; precursor DDVALEGVSHFFR (SEQ ID NO: 594) transition pair 497.91-469.3, 497.91-693.3, 497.91-849.4, or 497.91-978.5; precursor KPAEDEWGK (SEQ ID NO: 595) transition pair 353.84-297.2, 353.84-390.2, 353.84-541.3, or 353.84-634.3; precursor LGGPEAGLGEYLFER (SEQ ID NO: 596) transition pair 804.41-451.2, 804.41-525.3, 804.41-564.3, or 804.41-913.4; precursor LNQALLDLHALGSAR (SEQ ID NO: 597) transition pair 531.3-356.2, 531.3-427.2, 531.3-574.3, or 531.3-711.4; precursor MGDHLTNLHR (SEQ ID NO: 598) transition pair 398.53-312.2, 398.53-539.3, 398.53-640.53, or 398.53-753.4; precursor LYGSAGPPPTGEEDTAEK (SEQ ID NO: 663) transition pair 909.92-334.2, 909.92-492.2, 909.92-549.3, or 909.92-878.4; precursor TWNDPSVQQDIK (SEQ ID NO: 664) transition pair 715.85-260.2, 715.85-288.1, 715.85-517.2, or 715.85-914.5; precursor VYEGERPLTK (SEQ ID NO: 665) transition pair 397.88-458.3, 397.88-614.4, 397.88-800.5, or 397.88-929.5; precursor IIIQESALDYR (SEQ ID NO: 666) transition pair 660.86-338.2, 660.86-724.4, 660.86-853.4, or 660.86-981.5; precursor LDPALQDK (SEQ ID NO: 667) transition pair 450.25-503.3, 450.25-574.3, 450.25-671.4, or 450.25-786.4; precursor LIAQDYK (SEQ ID NO: 668) transition pair 425.74-310.2, 425.74-553.3, 425.74-624.3, or 425.74-737.4; precursor NDINILK (SEQ ID NO: 669) transition pair 415.24-260.2, 415.24-487.3, 415.24-600.4, or 415.24-715.4; precursor VAELSSDDFHLDR (SEQ ID NO: 670) transition pair 501.91-403.2, 501.91-540.3, 501.91-687.4, 501.91-702.3; precursor ALPGTPVASSQPR (SEQ ID NO: 839) transition pair 640.85-440.3, 640.85-440.3, 640.85-574.3, 640.85-841.5, or 640.85-999.5; precursor EVPLLQSLWLAHNEIR (SEQ ID NO: 840) transition pair 640.02-531.3, 640.02-668.3, 640.02-739.4, or 640.02-852.5; precursor LPGLPEGAFR (SEQ ID NO: 841) transition pair 528.8-268.2, 528.8-381.3, 528.8-676.3, or 528.8-846.4; precursor TVAAGALASLSHLK (SEQ ID NO: 843) transition pair 446.93-343.2, 446.93-684.4, 446.93-755.4, or 446.93-868.5; precursor ASHEEVEGLVEK (SEQ ID NO: 938) transition pair 442.89-545.3, 442.89-554.2, 442.89-653.3, or 442.89-674.4; precursor STHTLDLSR (SEQ ID NO: 940) transition pair 343.85-262.2, 343.85-375.2, 343.85-490.3, or 343.85-655.3; precursor VEIFYR (SEQ ID NO: 941) transition pair 413.73-338.2, 413.73-485.3, 413.73-598.3, or 413.73-727.4; precursor YSSDYFQAPSDYR (SEQ ID NO: 942) transition pair 799.84-637.3, 799.84-708.3, 799.84-836.4, or 799.84-983.5; precursor AALSGANVLTLIEK (SEQ ID NO: 958) transition pair 700.4-1057.6, 700.4-1144.7, 700.4-716.5, or 700.4-929.6; precursor TVLWPNGLSLDIPAGR (SEQ ID NO: 959) transition pair 854.972-1209.7, 854.972-400.231, 854.972-500.3, 854.972-500.3, or 854.972-605.333; precursor VFFTDYGQIPK (SEQ ID NO: 960) transition pair 657.84-1068.54, 657.84-244.2, 657.84-820.4, or 657.84-921.468; precursor ADEGISFR (SEQ ID NO: 1321) transition pair 447.72-409.2, 447.72-552.3, 447.72-579.3, or 447.72-708.4; precursor ATAVMPDGQFK (SEQ ID NO: 1322) transition pair 582.79-691.3, 582.79-822.4, 582.79-921.5, or 582.79-992.5; precursor DISLSDYK (SEQ ID NO: 1323) transition pair 470.74-310.2, 470.74-512.2, 470.74-625.3, or 470.74-712.4; precursor GLFIIDDK (SEQ ID NO: 1324) transition pair 460.76-377.2, 460.76-490.3, 460.76-603.3, or 460.76-750.76; precursor IGHPAPNFK (SEQ ID NO: 1325) transition pair 327.52-294.2, 327.52-505.3, 327.52-576.3, or 327.52-673.4; precursor LVQAFQFTDK (SEQ ID NO: 1326) transition pair 598.82-341.2, 598.82-785.4, 598.82-856.4, or 598.82-984.5; precursor QGGLGPMNIPLVSDPK (SEQ ID NO: 1327) transition pair 811.93-356.2, 811.93-446.2, 811.93-545.3, or 811.93-658.4; precursor QITVNDLPVGR (SEQ ID NO: 1328) transition pair 606.34-428.3, 606.34-770.4, 606.34-869.5, or 606.34-970.5; precursor SVDETLR (SEQ ID NO: 1329) transition pair 410.21-288.2, 410.21-389.3, 410.21-518.3, or 410.21-633.3; precursor TIAQDYGVLK (SEQ ID NO: 1330) transition pair 554.31-579.4, 554.31-694.4, 554.31-822.4, or 554.31-893.5; precursor GGTLSTPQTGSENDALYEYLR (SEQ ID NO: 1588) transition pair 758.03-451.3, 758.03-580.3, 758.03-856.5, or 758.03-927.5; precursor LDTLAQEVALLK (SEQ ID NO: 1589) transition pair 657.39-330.2, 657.39-543.4, 657.39-800.5, or 657.39-871.5; precursor NWETEITAQPDGGK (SEQ ID NO: 1590) transition pair 773.36-301.1, 773.36-430.2, 773.36-473.2, or 773.36-886.5; precursor FQDLVDAVR (SEQ ID NO: 1641) transition pair 531.78-391.2, 531.78-559.3, 531.78-672.4, or 531.78-787.4; precursor FTGSQPFGQGVEHATANK (SEQ ID NO: 1642) transition pair 625.97-261.2, 625.97-504.3, 625.97-521.2, or 625.97-926.5; precursor FVFGTIPEDILR (SEQ ID NO: 1643) transition pair 465.58-288.2, 465.58-516.3, 465.58-645.4, or 465.58-742.4; precursor GFLLLASLR (SEQ ID NO: 1644) transition pair 495.31-318.2, 495.31-375.2, 495.31-446.3, or 495.31-559.4; or precursor GTSQNDPNWVVR (SEQ ID NO: 1645) transition pair 686.83-373.3, 686.83-770.4, 686.83-885.5, or 686.83-999.5, wherein each of the precursor ions has a double or triple charge.