Patent ID: 8232081
Filing Date: 2012-07-31
Classification: C12N,C12P

Abstract:
1. A method of producing pantoate and/or pantothenate, comprising culturing a microorganism under conditions such that pantoate and/or pantothenate is produced, wherein the microorganism comprises a recombinant vector comprising a panE1 gene and an artificial ribosome binding site (RBS) comprising the nucleotide sequence set forth in SEQ ID NO: 50 operably linked to the panE1 gene, wherein the panE1 gene comprises: (a) the nucleotide sequence of SEQ ID NO: 29; (b) a nucleotide sequence encoding the polypeptide sequence of SEQ ID NO: 30; or (c) a nucleotide sequence encoding a polypeptide sequence having at least 95% or greater amino acid sequence identity to the polypeptide sequence of SEQ ID NO: 30 and having ketopantoate reductase activity.