Patent ID: 7582318
Filing Date: 2009-09-01
Classification: A01N,A23B,A61P

Abstract:
1. An extract comprising flavonoids and/or phenols from monofloral ulmo honey ( Eucryphia cordifolia ), said extract comprising gallic acid in the amount of 0.236±0.005 μg/ml, coumaric acid in the amount of 0.221±0.005 μg/ml, pherulic acid in the amount of 0.235±0.005 μg/ml, salicylic acid in the amount of 0.435±0.005 μg/ml, naringenin in the amount of 0.456±0.005 μg/ml and kaempherol in the amount of 0.174±0.005 μg/ml, with a pH: 4.9 to 5.5, wherein said extract is prepared by a process comprising: a) weighing a quantity of honey; b) diluting said honey in distilled water acidified with HCI (pH=2) to form a solution; c) putting the solution in a volumetric flask and filling the flask up with additional acid water; d) filtering said solution obtained in step c) and passing it through a column of cationic exchange at a constant dropping speed for phenolic compounds to be retained in said column; e) cleaning said column with acid water and discarding the acid water that passes through the column; f) cleaning said column a second time with neutral distilled water and discarding the neutral distilled water that passes through the column; g) cleaning for a third time with pure methanol to elute the phenolic compounds from the column, gathering methanol elute, and rotoevaporating the methanol elute to vacuum dryness at a temperature of 45° C., in order to eliminate the methanol and concentrate the obtained phenolic compounds to form a residue in a rotoevaporator; h) re-suspending the residue in distilled water to form a suspension; i) extracting the suspension 3 times with diethyl ether to form a diethyl ether extraction solution; j) concentrating said diethyl ether extraction solution to dryness in a rotoevaporator to form a second residue; k) re-suspending the second residue in sterilized distilled water; l) filtering the resuspended residue through a nitrocellulose syringe filter of 20 μm and storing the filtered residue at −20° C.