Patent ID: 8852903
Filing Date: 2014-10-07
Classification: C07K,C12P

Abstract:
1. A recombinant microorganism comprising a promoter operably linked to a native or non-native nucleotide sequence, wherein the recombinant microorganism is PTS (phosphoenolpyruvate-suqar phosphotransferase) minus and has increased fructokinase activity, and wherein the native or non-native nucleotide sequence is selected from the group consisting of: (a) a nucleotide sequence encoding a fucose: H (b) a nucleotide sequence encoding a fucose: H (c) a nucleotide sequence encoding a fucose: H wherein the promoter is one that is not normally associated with the nucleotide sequence encoding a fucose: H + symporter polypeptide, the nucleotide sequence is overexpressed in the recombinant microorganism, the recombinant microorganism has an improved ability to co-metabolize in medium both fructose and glucose compared to an equivalent microorganism lacking the promoter operably linked to the nucleotide sequence, and further wherein the recombinant microorganism produces an increased amount of 1,3-propanediol, glycerol, or 3-hydroxypropionic acid when grown in a medium containing glucose and fructose as compared to an equivalent microorganism lacking the promoter operably linked to the nucleotide sequence.