Patent ID: 6333161
Filing Date: 2001-12-25
Classification: A61P,C07K,F02B

Abstract:
A method of assaying a test ligand for the ability to bind to a human CNS receptor, said method comprising the steps of(a) incubating a test ligand with: a genetically engineered cell that produces a kainate-binding human EAA5 receptor selected from the group consisting of:the human EAA5a receptor encoded by residues 1-888 of SEQ ID NO:2;the human EAA5b receptor encoded by residues 1-888 of SEQ ID NO:2 wherein residue 321 is arginine; andthe human EAA5c receptor encoded by residues 1-838 of SEQ ID NO:2 followed by two proline residues;said cell having incorporated expressibly therein a heterologous DNA molecule encoding a human EAA5 receptor or,a membrane preparation derived from said cell, wherein said incubation is performed under conditions which permit binding of said test ligand with said human EAA5 receptor; and(b) determining the extent of binding between said test ligand and said human EAA5 receptor by comparison with kainate-binding to said EAA5 receptor.