Patent ID: 8389273
Filing Date: 2013-03-05
Classification: B01L

Abstract:
1. A polymerase chain reaction (PCR) droplet device, comprising: a cooling plate; a substrate disposed on the cooling plate; a first containing area disposed on the substrate to contain a liquid comprising an analyzer; a second containing area disposed on the substrate, and the second containing area surrounding the first containing area to contain a hydrophobic solution, wherein the hydrophobic solution is located at a surface of the liquid; a heating coil disposed on a center of the first containing area and the heating coil is located inside the first containing area; at least one conducting wire disposed on the substrate and connected with the heating coil; and a plurality of sensors disposed around the heating coil to detect temperature changes of the heating coil; wherein, the analyzer comprises a template, a primer, a deoxyribonucleotide triphosphate (dNTP) and a polymerase; the at least one conducting wire is applied with an electric current or a voltage to heat the heating coil such that buoyancy in an inside of the liquid is generated, thereby driving the analyzer to move to a top of the inside of the liquid and subsequently to move to a periphery of the inside of the liquid so as to form a thermal cycle; and the template is amplified by recycling the thermal cycle; wherein, a metal electrical conductivity of the heating coil is higher than a metal electrical conductivity of the at least one conducting wire.