Patent ID: 6964872
Filing Date: 2005-11-15
Classification: G01N

Abstract:
1. A quantitative immunoassay using an insoluble carrier particle which comprises (i) providing an insoluble magnetic carrier particle in a state substantially free of any adsorbed antigen and/or antibody, (ii) adsorbing an antigenic substance in a test sample on said insoluble magnetic carrier particle or binding said antigenic substance to said insoluble magnetic carrier particle, (iii) reacting said insoluble magnetic carrier particle with a labeled antibody specifically reactive with a solid-phase form of said antigenic substance, but substantially non-reactive with a native-state form of said antigenic substance, wherein said solid-phase form antigenic substance is attached to said insoluble magnetic carrier particle, and said native-state antigenic substance is in a liquid phase, (iv) washing said insoluble magnetic carrier particle, and (v) measuring the label on said labeled antibody captured by said solid-phase antigenic substance, wherein said antigenic substance in the test sample is hemoglobin antigen HbA1c.