Patent ID: 6872551
Filing Date: 2005-03-29
Classification: C07K,C12N

Abstract:
1. A method of expressing a recombinant poly-amino acid of interest, the method comprising: i.) providing a vector encoding a fusion protein comprising the recombinant poly-amino acid of interest located C-terminally to a purification tag and wherein the purification tag and the recombinant poly-amino acid of interest are separated by an expression enhancing amino acid sequence cleavable by Ulp1(403-621) protease, wherein the poly-amino acid of interest is expressed at 1% or less than 1% of total protein by weight or is expressed defectively when it does not contain an expression enhancing amino acid sequence in a recombinant expression system; and ii.) expressing the fusion protein from the vector in a suitable recombinant host cell.