Patent ID: 6194222
Filing Date: 2001-02-27
Classification: G01N,Y10S

Abstract:
A method for determining a rate of flow of a solution through an assay device, wherein said assay device during an assay for a predetermined analyte comprises a reaction chamber and at least one diagnostic lane, the method comprising:(a) providing a first member of a binding pair (MBP) in said reaction chamber and a second MBP bound to a solid phase on said diagnostic lane, wherein said first MBP comprises a label, wherein said first MBP and said second MBP do not bind to any analyte or to any reagents used for measuring the presence or amount of any analyte in said assay device during the assay for the predetermined analyte, and wherein said first MBP and said second MBP have specific binding affinity for one another;(b) detecting a signal from said diagnostic lane, wherein said signal is generated from said label; and(c) determining said rate of flow of liquid through said assay device during an assay for a predetermined analyte from said reaction chamber through said diagnostic lane from the amount of said signal in said diagnostic lane.