Patent ID: 6673364
Filing Date: 2004-01-06
Classification: A61K,Y10T

Abstract:
A method for increasing the rate of exchange of a bilayer stabilizing component from a liposome formulation to improve multiple dosing, said method comprising:(a) producing a first liposome composition having a lipid, a bioactive agent and a first bilayer stabilizing component, wherein said first bilayer stabilizing component is DSPE-PEG2000; (b) determining the rate of exchange of DSPE-PEG2000 from said first liposome composition using a fluorescent lipid mixing assay; and (c) preparing a second liposome formulation nearly identical to said first liposome formulation having said lipid, said bioactive agent, but substituting DSPE-PEG2000 from said first liposome composition with a second bilayer stabilizing component having a faster rate of exchanges wherein said second liposome composition results in improved dosing.