Patent ID: 6713294
Filing Date: 2004-03-30
Classification: C12N,C12Q,C12Y

Abstract:
An isolated cytosine DNA glycosylase (CDG) capable of releasing cytosine bases from single stranded (ss) DNA and double stranded (ds) DNA obtainable by modification of a uracil-DNA glycosylase (UDG), wherein asparagine (Asn) at amino acid position 204 in the human UDG protein encoded by the human uracil nucleic acid glycosylase gene (UNG1) (SEQ ID NO: 8), or by the alternatively spliced human uracil nucleic acid glycosylase gene (UNG2) (SEQ ID NO: 2), or an equivalent residue in a homologous UDG of another species, is replaced with an aspartic acid residue (Asp).