Patent ID: 8062845
Filing Date: 2011-11-22
Classification: C12N

Abstract:
1. A method of isolating RNA from a biological specimen comprising: (a) contacting the biological specimen with an admixture of (i) from about 750 to about 1000 microliters of a mono-phasic solution of phenol and guanidine isothiocyanate, and (ii) from about 100 to about 300 microliters of a lysis buffer under conditions and for a time appropriate to form a homogenate; (b) admixing the homogenate with a water-immiscible organic solvent under conditions and for a time appropriate to form an aqueous phase and an organic phase; (c) contacting the aqueous phase with a C (d) recovering the precipitated RNA wherein the lysis buffer compromises a chelating agent and a dispersing agent, and the biological specimen is a bacterium.