Patent ID: 7166446
Filing Date: 2007-01-23
Classification: A61K,A61L,A61P,C07K,C12N

Abstract:
1. A vector comprising a nucleotide sequence that comprises in the 5′ to 3′ direction and operably linked (a) a yeast-recognized transcription and translation initiation region, (b) a coding sequence for a hybrid precursor polypeptide, and (c) a yeast-recognized transcription and translation termination region, wherein said hybrid precursor polypeptide comprises: wherein: SP comprises a signal peptide sequence for a yeast secreted protein; PS comprises a preferred processing site cleaved in vivo by a yeast proteolytic enzyme; LP comprises a leader peptide sequence for a yeast secreted protein; NPRO MHP comprises a peptide sequence for said mature heterologous mammalian protein of interest; CPRO n-1, n-2, n-3, and n-4 independently=0 or 1; wherein said mammalian protein is a native PDGF-AA protein or variant thereof, said variant having an amino acid sequence that has at least 70% sequence identity to the amino acid sequence of said native PDGF-AA protein, wherein said processing sites allow for proteolytic processing of said precursor polypeptide to said mature protein in vivo by a yeast host cell, and wherein at least n-3 or n-4=1.