Patent ID: 6479262
Filing Date: 2002-11-12
Classification: C07B,C07H,C12N,C12P,C40B

Abstract:
A method of preparing a polynucleotide having at least 200 nucleotides and a predetermined nucleotide sequence comprising the steps:a) providing a solid support; b) providing a plurality of oligonucleotides, wherein the combination of the nucleotide sequences of said oligonucleotides comprises the nucleotide sequence of said polynucleotide; c) contacting said solid support with the 3â€² terminus of a first oligonucleotide from said plurality of oligonucleotides to form a tethered oligonucleotide; d) ligating the 3â€² terminus of another oligonucleotide from said plurality of oligonucleotides to the 5â€² terminus of said tethered oligonucleotide, wherein said ligating is carried out in the presence of RNA ligase or ribozyme; e) phosphorylating the 5â€² terminus of said another oligonucleotide; and f) repeating steps d) and e) until said polynucleotide is prepared.