Patent ID: 8993281
Filing Date: 2015-03-31
Classification: C07K,C12N,C12P,Y02E

Abstract:
1. A genetically modified Acinetobacier host for lipid production, wherein (a) the host has been modified genetically by transformation and recombination to be deficient of gene ACIAD 2177 (SEQ ID NO:4) or a gene haying at least 95% sequence identity to ACIAD 2177 (SEQ ID NO:4). and (b) the host further has been modified genetically by transformation and recombination to be deficient of one or more genes selected from a group consisting of a gene encoding fatty acyl-CoA reductase (EC1.2.1,n2), gene ACIAD 3383 (SEQ ID NO:1) or a gene having at least 95% sequence identity to ACIAD 3383 (SEQ ID NO: 1): a gene encoding lipase (EC:3.1.1.3) gene ACIAD 3309 (SEQ ED NO: 2) or a gene having at least 95% sequence identity to ACIAD 3309 (SEQ ID NO:2) a gene encoding pyruvate dehydrogenase (EC:1.2.2.2), gene ACIAD 3381 (SEQ ID NO:3) or a gene haying at least 95% sequence identity to ACIAD 3381 (SEQ ID NO:3); a gene encoding diacylglycerol kinase (EC:2.7.1.107), gene ACIAD 2837 (SEQ ID NO:5 or a gene having at least 95% sequence identity to ACIAD 2837 (SEQ ID NO:5); a gene encoding succinate dehydrogenase (EC:1.3.5.1), gene ACIAD 2880 (SEQ ID NO:6) or a gene having at least 95% sequence identity to ACIAD 2880 (SEQ ID NO:6); a gene encoding glycerol-3-phosphate dehydrogenase (EC 1.1.5.3), gene ACIAD 2844 (SEQ ID NO:7) or a gene having at least 95% sequence identity to ACIAD 2844 (SEQ ED NO:7); a gene encoding cytochrome o ubiquinol oxidase subunit II (EC:1.10.3.˜), gene ACIAD 2425 (SEQ ID NO:8) or a gene having at least 95% sequence identity to ACLAD 2425 (SEQ ID NO:8); a gene encoding cytochrome o ubiquinol oxidase subunit I (EC:1.10.3.˜), gene ACIAD 2426 (SEQ ID NO:9), or a gene having at least 95% sequence identity to ACIAD 2426 (SEQ ID NO:9); a gene encoding cytochrome d terminal oxidase polypeptide subunit II (EC1.10.3.˜), gene ACIAD 2291 (SEQ ID NO:10, or a gene having at least 95% sequence identity to ACIAD 2291 (SEQ ID NO:10); a gene encoding carboxylesterase (EC:3.1.1.1), gene ACIAD 3648 (SEQ ID NO:11), or a gene having at least 95% sequence identity to ACRID. 3648 (SEQ ID NO: 11), a gene encoding esterase, gene ACIAD 1134 (SEQ ID NO:12), or a gene haying at least 95% sequence identity to ACIAD 1134 (SEQ ID NO:12), various lipases (EC:3.1.1.3), a gene ACIAD 1121 (SEQ ID NO: 13) or a gene having at least 95% sequence identity to ACIAD 1121 (SEQ ID NO:13), and a gene encoding acyl-CoA synthetase (EC:6.2.1.3), gene ACIAD 0235 (:SEQ ID NO:14) or a gene having at least 95% sequence identity to ACIAD 0235 (SEQ ID NO:14), wherein said host has increased production of wax ester, triacylglycerols, or total lipids compared to the unmodified