Patent ID: 6261786
Filing Date: 2001-07-17
Classification: A01K,A61K,C07K,C12N

Abstract:
An assay for identifying compounds able to potentiate or inhibit biding of a hedgehog polypeptide to a naturally occurring patched receptor, comprising:(a) forming a reaction mixture including:(i) a vertebrate hedgehog polypeptide;(ii) a naturally occurring patched receptor; and(iii) a test compound; and(b) detecting binding of the hedgehog polypeptide and the patched receptor;wherein a change in the binding of the hedgehog polypeptide and the patched receptor in the presence of the test compound, relative to the binding in the absence of the test compound, indicates that said test, compound potentiates or inhibits binding of said hedgehog polypeptide to said patched receptor.