Patent ID: 8748090
Filing Date: 2014-06-10
Classification: C12Q,G01N

Abstract:
1. A method of screening for hybridization comprising the steps of: preparing multiple sets of uniquely identifiable microparticles wherein the microparticles within a set have the same unique identifiable characteristic and further, wherein one or more sets comprise two or more subsets of microparticles each of said subsets presenting at least one unique sequence specific oligonucleotide probe (SSO) or primer that hybridizes to a target nucleic acid molecule within a biological sample, contacting said multiple sets of microparticles with a biological sample under conditions in which a target nucleic acid molecule within the biological sample hybridizes to the probe or primer, detecting the hybridization of a target nucleic acid molecule within the biological sample and the probe or primer on said microparticles by generation of a signal indicative of the hybridization between the target nucleic acid molecule and the probe or primer, measuring the signal indicative of the hybridization of said microparticles, selecting a proportion of microparticles exhibiting the greatest intensity of signal indicative of the hybridization for one or more sets of uniquely identifiable microparticles for decreasing the risk of false negative events, wherein the proportion is less than 100% of the microparticles exhibiting a signal indicative of the hybridization within a set of uniquely identifiable microparticles, determining for said set of uniquely identifiable microparticles whether the signal for the selected proportion is greater than a selected threshold indicative of the presence of one or more target nucleic acid molecules in said biological sample; and identifying the uniquely identifiable microparticle sets indicating hybridization indicative of the presence of one of more target nucleic acid molecules in said biological sample.