Patent ID: 7626081
Filing Date: 2009-12-01
Classification: C12N

Abstract:
1. A method for obtaining a plant which produces at least one seed having a protein content different from a plant of the same species not obtained by this method, said method comprising: (a) transforming a host plant cell with a DNA construct, wherein said construct comprises, as operably linked components, a cotton alpha globulin gene regulatory region comprising a promoter containing a portion of SEQ ID NO: 1 that is capable of promoting seed-specific expression, wherein said portion comprises the cis-elements of SEQ ID NO: 1 which confers seed-specific expression to the promoter, and a DNA sequence encoding a protein, wherein said components are functional in a plant cell, whereby said DNA construct becomes integrated into the genome of said plant cell; (b) regenerating a plant from said transformed plant cell; (c) obtaining seeds from the regenerated plant of step (b); (d) selecting the seeds of step (c) for seed-specific expression of said DNA sequence; (e) growing plants from the selected seeds of step (d); (f) subjecting plants of step (e) to water stress; (g) selecting a plant from the plants of step (f) that does not express said DNA sequence in tissues other than seeds, and wherein said selected plant produces at least one said seed that has said protein content different from a plant of the same species not transformed by said DNA construct.