Patent ID: 8143009
Filing Date: 2012-03-27
Classification: C12Q,G01N

Abstract:
1. A first pass method of predicting the type of toxicity of a chemical compound of unknown toxicity, the method comprising the steps of: a) contacting an isolated population of mammalian liver stem cells (LSCs) with the chemical compound of unknown toxicity; b) creating a molecular profile identifying the alterations in gene expression in the population of LSCs contacted with the chemical composition of unknown toxicity compared to LSCs in contact with culture medium only; c) comparing the molecular profile of LSCs contacted with the chemical compound of unknown toxicity to sets of commonly altered RNAs identified from two or more molecular profiles of two or more isolated populations of LSCs wherein each isolated population of LSCs is contacted with one chemical composition of known toxicity, wherein the sets of commonly altered RNAs of the chemical compositions having known liver toxicity have been identified by d) predicting toxicity of the chemical composition of unknown toxicity wherein common alterations in gene expressions between the sets of commonly altered RNAs for chemical compounds of similar types of toxicity and the molecular profile from the LSCs contacted with the compound of unknown toxicity indicate the potential correlation of toxicity between the two.