Patent ID: 6951742
Filing Date: 2005-10-04
Classification: A61K,C07K,C12N,C12Q,Y02A

Abstract:
1. A process for assessing a proteomics profile of a set of pre-selected DNA sequences isolated from a bio-sample, which comprises: 1) cloning each of said DNA sequences into a dual-expression vector that is capable of expressing said DNA sequences in chicken cells, non-chicken cells or in vitro expression systems; 2) delivering said DNA sequence in said dual-expression vector formed in step 1), or protein encoded by said DNA sequence, or a mixture thereof, to a chicken in an amount sufficient to induce detectable production of antibodies to an antigen encoded by said DNA sequence, and recovering said antibodies from said chicken; 3) delivering said DNA sequence, which is delivered to said chicken in step 2), to said non-chicken cells, and recovering proteins or peptides encoded by said DNA sequence from said non-chicken cells, or expressing and recovering proteins or peptides encoded by said DNA sequence in said in vitro expression systems; 4) conducting immunoreactions between said antibodies recovered in step 2) with said proteins or peptides recovered from step 3) to characterize the immunospecificity of said antibodies; and 5) conducting immunoreactions between said antibodies recovered in step 2) with said bio-samples to determine the proteomics profile of said set of pre-selected DNA sequences.