Patent ID: 8470549
Filing Date: 2013-06-25
Classification: C12Q,G01N

Abstract:
1. A method for detecting an antigen, the method comprises steps of: (a) immersing particles in a first buffer solution which is predicted to contain the antigen; wherein an antibody and a multi-copper oxidase CueO are immobilized on each surface of the particles, and the antibody reacts specifically with the antigen; (b) recovering the particles obtained in the step (a); (c) mixing the particles recovered in the step (b) with 2,2′-Azinobis(3-ethylbenzothiazoline-6-sulfonic Acid (ABIS), and a second buffer solution so as to prepare a reaction solution; wherein: the second buffer solution contains a substrate of the multi-copper oxidase CueO, the second buffer solution has a pH of not less than 4.5 and not more than 5.5, and the second buffer solution has an ion strength falling within the range of not less than 0.3 mM and not more than 1.0 mM; (d) measuring an activity degree of the multi-copper oxidase CueO contained in the reaction solution obtained in the step (c) by an absorbance measurement technique; and (e) determining that the first buffer solution contains the antigen if the following formula is satisfied: the activity degree measured in the step (d) ≧1.4×(blank value) wherein the blank value represents the activity degree of the multi-copper oxidase CueO measured by an absorbance measurement technique in which the antigen is not used, however, the particles and the second buffer solution are used.