Patent ID: 6399054
Filing Date: 2002-06-04
Classification: A61K,C12N

Abstract:
A method for producing MHC-restricted, activated tumor-specific T cells, comprising the following steps:(1) obtaining tumor cells from a patient; (2) inhibiting the proliferation of the tumor cells and incubating said tumor cells with reagents comprising a ternary complex consisting essentially of (i) a biotinylated antibody against a cell surface antigen of said tumor cells from said patient or at least two Fab fragments of said antibody, (ii) a ligand which is avidin, streptavidin or neutravidin, and (iii) biotinylated B7-Ig, to generate targeted tumor cells;(3) obtaining T cells from said patient; thereafter (4) co-cultivating said targeted tumor cells with said T cells to activate said T cells in the presence of at least interleukin 2; and thereafter (5) isolating said activated T cells from the targeted tumor cells to obtain activated tumor-specific T cells.