Patent ID: 9164109
Filing Date: 2015-10-20
Classification: G01N

Abstract:
1. A method of determining a sample probe response profile of two or more probes of unbound free fatty acids (FFAu) in body fluids for a test individual comprising: measuring a fluorescence index for the two or more probes of FFAu wherein the fluorescence index comprises fluorescence intensity, ratio of intensities at two excitation and/or emission wavelengths, polarization and/or lifetime for the two or more probes, and wherein each of the two or more probes comprise a mutant intracellular lipid binding protein labeled with a fluorophore, and wherein the two or more probes each have different specificities for different FFAu and generate a change in fluorescence index upon binding FFAu; obtaining a body fluid sample from the test individual; contacting the body fluid sample with the two or more probes of FFAu; measuring a sample response for each of the two or more probes in the body fluid sample to generate the sample probe response profile, wherein measuring the sample response comprises measuring the change in the fluorescence index as a result of contact with the body fluid sample with each of the two or more probes; and applying a statistical classification analysis to determine the probability that the sample response profile corresponds to a state different from a state of a preselected control population.