Patent ID: 7125665
Filing Date: 2006-10-24
Classification: C12Q

Abstract:
1. A method for detecting the presence or absence of a target nucleic acid sequence in a sample, said method comprising: a) providing in a PCR reaction mixture a sample suspected to contain a target nucleic acid sequence, oligonucleotide PCR primers that hybridize to the target nucleic acid sequence for PCR amplification of said target sequence, each of four deoxynucleoside triphosphates, nucleic acid polymerase having 5′ to 3′ exonuclease activity and devoid of 3′ to 5′ exonuclease activity, and an EPR-labeled oligonucleotide analytical probe (EPR probe); b) amplifying target nucleic acid sequence in the sample under suitable PCR reaction mixture temperature conditions by a repetitive series of PCR thermal cycling steps comprising: c) measuring the EPR resonance signals produced by degradation of EPR-labeled probes by magnetic resonance as a measure of the presence of the target nucleic acid sequence following amplification of the target nucleic acid sequence by one or more series of thermal cycling steps; wherein the 5′ EPR labeled nucleotide fragments produced are measured by measuring EPR signal h