Patent ID: 6992183
Filing Date: 2006-01-31
Classification: A61K,A61P,C07H,C08B,Y02A

Abstract:
1. A process for the preparation of N,O-oversulfated K5 polysaccharide having a sulfation degree higher then 3.2, which comprises (a) treating a K5 polysaccharide from fermentation with isopropanol in a 2–5M saline aqueous solution to purify the K5; (b) submitting the thus purified K5 polysaccharide to a N-deacetylation followed by a subsequent N-sulfation by treatment with an N-sulfating agent; (c) treating an ammonium salt of the N-sulfate K5 polysaccharide thus obtained with an O-sulfating agent under O-oversulfation conditions; and (d) if necessary, submitting the product thus obtained to a N-sulfation and isolating the N,O oversulfated K5 polysaccharide.