Patent ID: 8759026
Filing Date: 2014-06-24
Classification: C12N,C12Y

Abstract:
1. A method for increasing recovery of an ADAMTS activity from a cell culture supernatant, the method comprising the steps of: (a) culturing a cell harboring a nucleic acid encoding an ADAMTS protein in a culture medium; (b) removing a fraction of the supernatant from the culture; (c) performing a filtration or centrifugation step to remove residual cells from the fraction of the supernatant removed from the culture, thereby forming a clarified supernatant; (d) concentrating ADAMTS protein in the clarified supernatant by ultrafiltration, thereby forming a concentrated supernatant; and (e) diafiltering the concentrated supernatant against a buffer comprising at least about 0.5 μM zinc and at least about 0.1 mM calcium; thereby preparing an ADAMTS composition with increased recovery of ADAMTS activity.