Patent ID: 8232073
Filing Date: 2012-07-31
Classification: C12Q,G01N

Abstract:
1. A method of diagnosing an individual as having a disease or condition associated with abnormal glycan biosynthesis, degradation, or accumulation, the method comprising: (a) generating a biomarker comprising of one or more non-reducing end glycan residual compounds, wherein the biomarker is generated by treating a population of glycans, in or isolated from a biological sample from the individual, with at least one digesting glycan enzymes, wherein prior to enzyme treatment, the biomarker is not present in abundance in samples from individuals with the disease or condition relative to individuals without the disease or condition, and (b) using an analytical instrument to detect the presence of and/or measure the amount of the biomarker produced and displaying or recording the presence of or a measure of a population of the biomarker; wherein the presence of and/or measure of the amount of the biomarker is utilized to determine the presence, identity, and/or severity of the disease or condition.