Patent ID: 6020122
Filing Date: 2000-02-01
Classification: A61K,A61P,C07K,C12N,G01N

Abstract:
A method for detecting the presence of an anti-hepatitis C virus (HCV) antibody in a test sample comprising the following steps:(a) contacting at least one antigen specific for said antibody with a test sample under conditions that facilitate antigen/antibody complex formation, wherein said antigen is the recombinant glycosylated HCV protein encoded by plasmid 577 (p577) having the structure shown in FIG. 1 and has been isolated and purified from mammalian host cells transfected with p577; and(b) contacting the complex of step (a) with an indicator reagent comprising said antigen conjugated to an signal generating compound under conditions that facilitate antigen/antibody/indicator reagent complex formation,wherein the signal generated is an indication of the presence of said anti-HCV antibody in said test sample.