Patent ID: 6872574
Filing Date: 2005-03-29
Classification: C12Q,C40B,G01N,G16B,Y10T

Abstract:
1. A method for screening for the bloactivity of a candidate compound toward a group of related target proteins in a proteomic mixture of proteins from a cell, employing at least one probe, each probe comprising a reactive functionality group specific for said group of target proteins and a ligand, each probe of the formula: wherein: X is a ligand for binding to a reciprocal receptor and/or providing a detectable signal; L is an alkylene, oxyalkylene or polyoxyalkylene linking group, wherein said oxyalkylenes are of from 2 to 3 carbon atoms; F is a phosphonate or sulfonyl functional group reactive at an active site of a target enzyme; and R is bonded to F and a moiety of less than 1 kDal providing specific affinity for said enzymes, and when F is phosponate, R is fluorine and when F is sulfonyl, R is an aryl or heteroaryl group; said method comprising: