Patent ID: 6855500
Filing Date: 2005-02-15
Classification: C07H,C12N,C12P,C12Q

Abstract:
1. A method for genotyping a diploid organism, comprising: providing two alleles of a target gene of a diploid organism suspected to contain a polymorphism; obtaining a first segment of one allele wherein the segment includes the suspected polymorphic locus; obtaining a second segment from the other allele wherein the segment also includes the suspected polymorphic locus; replacing a first natural nucleotide, suspected to be at the polymorphic locus, at greater than 90% of its points of occurrence in the first and the second segment with a first modified nucleotide to give a first and a second modified segment; replacing a second natural nucleotide, suspected to be at the polymorphic locus, at greater than 90% of its points of occurrence in the first and the second modified segments with a second modified nucleotide to give a first and a second twice-modified segment, wherein: covalently bonding a first fluorophore that emits light at a first wavelength to the first modified nucleotide or a residue thereof; covalently bonding a second fluorophore that emits light at a second wavelength to the second modified nucleotide or a residue thereof; cleaving the first and second twice-modified segments at greater than 90% of the points of occurrence of each modified nucleotide to give a first and second set of fragments, one fragment of each segment comprising primer and a modified nucleotide or residue thereof; isolating the two primer-containing fragments; analyzing the isolated fragments for the emission of the first wavelength of light, the second wavelength of light, or both, wherein: