Patent ID: 7135618
Filing Date: 2006-11-14
Classification: C07K,C12N

Abstract:
1. A method of producing a transgenic plant having an increased level of total fatty acids in a seed of the transgenic plant comprising, transforming a plant cell with an expression vector comprising a lipid metabolism protein (LMP) nucleic acid and generating from the plant cell the transgenic plant that expresses the LMP nucleic acid and that has an increased level of total fatty acids in the seed of the transgenic plant, wherein the nucleic acid encodes a polypeptide that comprises a β-oxidation domain, and wherein the LMP nucleic acid comprises a nucleic acid sequence selected from the group consisting of: a) the isolated nucleic acid as defined in SEQ ID NO:3; b) an isolated nucleic acid encoding the polypeptide as defined in SEQ ID NO:4; c) an isolated nucleic acid that hybridizes under stringent conditions to the full-length nucleic acid sequence as defined in SEQ ID NO:3 or the nucleic acid encoding the polypeptide as defined in SEQ ID NO:4, wherein the stringent conditions comprise hybridization in a 6× sodium chloride/sodium citrate (SSC) solution at about 45° C., followed by at least one wash in a 0.2 × SSC, 0.1% sodium dodecyl sulfate (SDS) solution at 65° C.; d) an isolated nucleic acid having at least 95% sequence identity with the isolated full-length nucleic acid as defined in SEQ ID NO:3 or the nucleic acid encoding the polypeptide as defined in SEQ ID NO:4; and e) a nucleic acid complementary to the nucleic acid of any of a) through d) above.