Patent ID: 6287838
Filing Date: 2001-09-11
Classification: C07K,C12N

Abstract:
An isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of:(a) the nucleotide sequence of SEQ ID NO:1;(b) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:2;(c) a nucleotide sequence encoding a fragment of the amino acid sequence of SEQ ID NO:2 having phospholipase activity in a mixed micelle assay with 1-palmitoyl-2-[.sup.14 C]-arachidonyl-phosphatidylcholine as a substrate;(d) a nucleotide sequence which hybridizes under stringent conditions of 4.times.SSC at 65 degrees C. with the sequence of (a), (b) or (c) which encodes a peptide having activity in a mixed micelle assay with 1-palmitoyl-2-[.sup.14 C]-arachidonyl-phosphatidylcholine;(e) the nucleotide sequence of SEQ ID NO:3;(f) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:4;(g) a nucleotide sequence encoding a fragment of the amino acid sequence of SEQ ID NO:4 having activity in a mixed micelle assay with 1-palmitoyl-2-[.sup.14 C]-arachidonyl-phosphatidylcholine; and(h) a nucleotide sequence which hybridizes under stringent conditions of 4.times.SSC at 65 degrees C. with the sequence of (e), (f), or (g) which encodes a peptide having phospholipase activity in a mixed micelle assay with 1-palmitoyl-2-[.sup.14 C]-arachidonyl-phosphatidylcholine as a substrate.