Patent ID: 6218141
Filing Date: 2001-04-17
Classification: A61K,A61P,C07K,Y10S

Abstract:
A method for the production of an isolated and purified high molecular weight protein of non-typeable Haemophilus which is HMW1, encoded by a DNA sequence having the nucleic acid sequence recited in SEQ ID No: 1 and having an apparent molecular weight of about 125 kDa, which comprises:assembling an expression vector containing the nucleic acid sequence recited in SEQ ID No: 1 which encodes the high molecular weight protein, HMW1, and a promoter operatively coupled to said nucleic acid sequence of SEQ ID No: 1transforming a host cell with the expression vector,expressing the HMW1 protein in the host cell, andisolating and purifying the expressed HMW1 protein.