Patent ID: 8945836
Filing Date: 2015-02-03
Classification: C07H,C12N,C12Q

Abstract:
1. A method to determine whether a multi-component nucleic acid enzyme (MNAzyme) has catalytic activity, the method comprising: selecting a point between adjacent nucleotides of a nucleic acid enzyme catalytic core nucleotide sequence at which to split the catalytic core nucleotide sequence for generation of first and second partial catalytic core portions on opposing sides of the point; contacting a first component oligonucleotide comprising the first partial catalytic core portion between a substrate arm portion and a sensor arm portion, a second component oligonucleotide comprising the second partial catalytic core portion between a substrate arm portion and a sensor arm portion, an MNAzyme assembly facilitator, and a first substrate potentially capable of catalytic modification by the MNAzyme, under conditions permitting self-assembly of the MNAzyme and potential catalytic activity of the MNAzyme, determining whether the MNAzyme catalytically modifies the substrate,