Patent ID: 8309344
Filing Date: 2012-11-13
Classification: C12M

Abstract:
1. An automatic system for isolating and incubating circulating tumor cells, comprising: an operation device, said operation device comprising a fluid delivery controller, an environment/CO a cell isolation and culture system, said cell isolation and culture system connected with said operation device, said cell isolation and culture system comprising a fluid controller and a sheet together forming a closed fluid area, said fluid controller comprising a sample pre-processing area, a sample impurities leach area, a typical cell isolation area, a cell culture area and a plurality of fluid delivery pipes, said fluid delivery pipes connecting said sample pre-processing area to said sample impurities leach area, said sample impurities leach area to said typical cell isolation area and said typical cell isolation area to said cell culture area; an up-plate device, said up-plate device comprising a connector, a fluid delivery part and a plurality of fluid delivery apertures, said connector connected with said cell isolation and culture system, said fluid delivery part connected with said fluid delivery controller; an image capture device, said image capture device arranged with respect to said cell isolation and culture system to obtain magnified microscopy image of cells; an image/data processor, said image/data processor connected with said image capture device to receive said microscopy image of cells and obtain converted digital data of said microscopy image of cells; and a central data processor, said central data processor connected with said image/data processor to receive said digital data of said microscopy image of cells and analyze growth, change, and number of cells.