Patent ID: 7695922
Filing Date: 2010-04-13
Classification: G01N

Abstract:
1. A method of assaying the biochemical activity of a compound against a target ion channel comprising: selecting a cell line having a normal resting transmembrane potential corresponding to a selected voltage dependent state of said target ion channel, wherein said cell line is selected from one or more of CHL, LTK(−), CHO-K1, HEK-293, RBL, F11, and HL5; expressing said target ion channel in a population of cells of said selected cell line; exposing said population of cells to said compound; repetitively exposing said population of cells to one or more electric fields produced with extracellular electrodes so as to effect a controlled change in transmembrane potential of one or more cells of said population of cells; and monitoring, without using a patch clamp, changes in the transmembrane potential of said one or more cells of said population of cells.