Patent ID: 8034620
Filing Date: 2011-10-11
Classification: A61K,C12N

Abstract:
1. A method of producing a packaging cell line capable of producing a recombinant, helper-free retrovirus, comprising transfecting a host cell with: a first vector comprising a retroviral gag gene, wherein said gag gene encodes retroviral matrix, capsid and nucleocapsid proteins; a second vector comprising a portion of a retroviral pol gene, fused to a Vpr or Vpx gene, wherein said portion of said pol gene encodes a retroviral protease; a third vector comprising the remainder of the retroviral pol gene not contained in said second vector, fused to a Vpr or a Vpx gene, wherein said remainder of said pol gene encodes a retroviral reverse transcriptase and a retroviral integrase; and a fourth vector comprising a viral env gene.