Patent ID: 8142822
Filing Date: 2012-03-27
Classification: C07K

Abstract:
1. A process for the preparation of a canola protein isolate having an increased proportion of 2S canola protein, which comprises: (a) providing an aqueous solution of 2S and 7S proteins consisting predominantly of 2S protein, (b) salinating the aqueous solution to a conductivity of at least about 0.3 mS and adjusting the pH of the salinated aqueous solution to a value of about 2.0 to about 4.0 to isoelectrically precipitate 7S protein from the aqueous solution, (c) removing precipitated 7S protein from the aqueous solution, and (d) recovering from the aqueous solution a canola protein isolate having a protein content of at least about 90 wt % (N×6.25) d.b. and having an increased proportion of 2S canola protein compared to the aqueous solution of 2S and 7S proteins.