Patent ID: 8039038
Filing Date: 2011-10-18
Classification: A61L

Abstract:
1. A process for the preparation of protein mediated calcium hydroxyapatite (HAp) coating on metal substrate and the said process comprising the steps of: a) roughening and ultrasonic cleaning the surface of the metal substrate by using acetone, ethanol and deionized water by known method to obtain a cleaned metal substrate; b) drying the above said cleaned metal substrate, at a temperature in the range of 50-60° C., for a period of about 1-2 hours and immersing it in an aqueous solution of protein of concentration in the range of 4-10 wt %, at a temperature in the range of 40-50° C., at pH in the range of 6.5-7, for a period of about 2-4 hours, followed by washing repeatedly 3-4 times with water and drying it at a temperature in the range of 20-30° C., for a period of about 1-2 hours to obtain the dried surface treated metal substrate; c) immersing the above said dried surface treated metal substrate in a simulated body fluid [SBF(N)], for a period of 1-2 days, at a temperature of 35-37° C., at a pH in the range of 6.5-7 to obtain the nucleated hydroxyapatite coated metal substrate, followed by washing with deionised water for 3-4 times and drying the washed hydroxyapatite nucleated metal, at a temperature in the range of 20-30° C. for a period of about 1-2 hours to obtain the dried hydroxyapatite nucleated metal substrate; d) immersing the above said dried hydroxyapatite nucleated metal substrate in another simulated body fluid [SBF (O)], for a period in the range of 2-4 days, at temperature of 35-37° C., at a pH in the range of 6-6.5, following by washing with deionised water for 3-4 times and drying it at a temperature of 20-30° C., for a period of 1-2 hours to obtain the desired protein mediated calcium hydroxyapatite (HAp) coating on hydroxyapatite nucleated metal substrate.