Patent ID: 8697357
Filing Date: 2014-04-15
Classification: C12Q,Y10T

Abstract:
1. A method of photocoupling methylcytosine by using nucleic acids, comprising: hybridizing both (i) a nucleic acid or a peptide nucleic acid immobilized on a carrier, and (ii) a test nucleic acid having methylcytosine to a template nucleic acid or a template peptide nucleic acid, thereby closely arranging the nucleic acid or peptide nucleic acid immobilized on the carrier and the test nucleic acid having methylcytosine to the template nucleic acid or template peptide nucleic acid, wherein the nucleic acid or peptide nucleic acid has a group represented by the Formula (I), (II), (III) or (IV) as a base moiety at a 5′ end thereof, wherein in the Formula (I), X represents O, S or NH, wherein in the Formula (I), R wherein in the Formula (I), R wherein in the Formula (II), R wherein in the Formula (II), R wherein in the Formula (III), Y represents O, S or NH, wherein in the Formula (III), Z represents NH wherein in the Formula (III), R wherein in the Formula (III), R wherein in the Formula (IV), R wherein in the Formula (IV), R performing light irradiation on the test nucleic acid and the immobilized nucleic acid or peptide nucleic acid that are arranged closely by the hybridization to the template nucleic acid or template peptide nucleic acid, thereby photocoupling the test nucleic acid to the immobilized nucleic acid or peptide nucleic acid.