Patent ID: 8512955
Filing Date: 2013-08-20
Classification: C12Q

Abstract:
1. A method for simultaneously amplifying at least two different target nucleic acid sequences contained in a sample comprising the steps of: (a) contacting a sample with at least two different target capture oligomers and with at least two different amplification oligomer complexes; (b) contacting said at least two target capture oligomers in step (a) with a solid support and immobilized probe for performing a target capture; (c) washing said sample after target capture; (d) pre-amplifying said target nucleic acid sequences using said amplification oligomer complexes, under conditions configured to maintain the amplification oligomer complexes, thereby generating first amplification products for each target nucleic acid hybridized by an amplification oligomer complex; (e) splitting said pre-amplified sample into at least two separate secondary target specific amplification reactions; (f) amplifying at least two of said pre-amplified samples generated in step (d) using target specific amplification oligomers that are not joined, thereby generating second amplification products.