Patent ID: 6316255
Filing Date: 2001-11-13
Classification: A61K,C07K,C12N,Y10S

Abstract:
Transduced cultured hepatocytes capable of expressing incorporated genetic material of interest in vivo, said hepatocytes produced by:(a) contacting cultured hepatocytes with media containing helper-free infectious recombinant retrovirus having a recombinant genome comprised of the incorporated genetic material of interest, a 5' LTR, a splice donor site downstream of the 5' LTR, a splice acceptor site upstream of the incorporated genetic material of interest, and a 3' LTR, wherein the genetic material of interest encodes a hormone, a receptor, or an enzyme; and(b) maintaining the cultured hepatocytes and the media containing the infectious recombinant retrovirus under conditions appropriate for infection of the hepatocytes.