Patent ID: 8796037
Filing Date: 2014-08-05
Classification: C07D,C07K,G01N

Abstract:
1. A method for detecting, separating and identifying an expressed trace protein and/or peptide in a test sample, comprising: converting a protein and/or peptide in a test sample to a fluorescent derivative by labeling said protein and/or peptide with a fluorescent derivatization reagent, wherein said fluorescent derivatization reagent, which does not fluoresce itself, is one selected from the group consisting of DAABD-X, TAABD-X, DAABSeD-X, TAABSeD-X, DAABThD-X, and TAABThD-X, wherein X represents Cl or F subjecting the labeled protein and/or peptide to one-dimensional or two-dimensional HPLC/fluorescence detection, to obtain fluorescent fractions, applying the fluorescent fractions to enzymatic hydrolysis to obtain digested peptide fragments, subjecting the digested peptide fragments to second stage HPLC/fluorescence detection to obtain a fluorescent chromatogram, applying peptide fractions of the fluorescent chromatogram to mass spectrometry or MS/MS analysis, collating the mass spectrometry or MS/MS data with a database, and providing said collated data for structural analysis to identify the expressed protein and/or peptide.