Patent ID: 8313949
Filing Date: 2012-11-20
Classification: G01N

Abstract:
1. A method for evaluating the activity of a test compound to induce phospholipidosis in a target subject, comprising obtaining (1) a test sample from a test subject that has been administered a test compound, (2) a population of test cells that have been contacted with the test compound, or (3) endocytic vesicles isolated from the test sample or the test cells, determining the level of each of a group of biomarkers in the test sample, the cells, or the endocytic vesicles, wherein the group of biomarkers includes 2,2′ di-22:6-BMP, 3,2′ di-22:6-BMP, 2,3′ di-22:6-BMP, and 3,3′di-22:6-BMP, and optionally includes di-22:6-PG, 2-mono-22:6-BMP, or 3-mono-22:6-BMP, and comparing the level of each biomarker with a corresponding predetermined level for the same biomarker, the predetermined level being obtained, for (1), from a control sample from a control subject who has not been administered the test compound, for (2), from a control cell that has not been contacted with the test compound, or for (3), from endocytic vesicles isolated from the control sample or the control cells, wherein the test compound is determined to have the activity to induce phospholipidosis in the target subject if the level of any of the group of biomarkers is at or above the corresponding pre-determined level.