Patent ID: 6180388
Filing Date: 2001-01-30
Classification: C12N,C12R

Abstract:
An isolated or substantially purified enzyme with amidase activity, which is a native Comamonas bacterium enzyme with amidase activity or an enzyme expressed by a recombinant bacterium comprising a DNA sequence encoding a native Comamonas bacterium enzyme with amidase activity, said enzyme being active with respect to (poly)amide substrates having the following formula (I): ##STR11##in which:A and B are monomer units,R.sup.1 and R.sup.3 are identical or different divalent radicals representing a substituted or unsubstituted, linear or branched (cyclo)alkylene, an arylene, or an arylalkylene, the aromatic radicals optionally being polycondensates and the number of carbons in the alkylenes being greater than or equal to 4,R.sup.2 corresponds to identical or different radicals selected from hydrogen and alkyl radicals having from 1 to 6 carbons,X iseither X.sup.1 is OH, OM or OR.sup.4, where M is selected from metals, and R.sup.4 is a linear or branched alkyl comprising from 1 to 6 carbon atoms,or X.sup.2 is ##STR12##where R.sup.2 and R.sup.3 are as defined above and R.sup.5 and R.sup.6, which are identical or different, have the same definition as that given above for R.sup.2,Y is:either Y.sup.1 is hydrogen,or Y.sup.2 is ##STR13##where R.sup.1 is as defined above and Z is hydrogen, M.sup.1 defined in the same way as M, or R.sup.4,whereinif X is X.sup.1, then Y is Y.sup.1 or Y.sup.2, or,if X is X.sup.2, then Y is Y.sup.2,and p is between 1 and 4;and it is capable of converting said substrate (I) to monomers A and monomers B.