Patent ID: 6291662
Filing Date: 2001-09-18
Classification: A61K,C07K,C12N

Abstract:
A DNA sequence encoding an analog of a mammalian serine protease inhibitor protein, said analog comprising at least eight cysteine residues and possessing serine protease inhibitor activity, wherein at least one active site comprises an amino acid sequence selected from the group consisting of amino acid sequences:Gln-Cys-Leu-R.sub.2 -Tyr-Lys-Lys-Pro-Glu-Cys-Gln-Ser-Asp; andGln-Cys-R.sub.8 -R.sub.3 -R.sub.9 -Asn-Pro-Pro-Asn-Phe-Cys-Glu-R.sub.4 -Asp whereinR.sub.2, R.sub.3 and R.sub.4 are the same or different and are selected from the group consisting of methionine, valine, alanine, phenylalanine, tyrosine, tryptophan, lysine, glycine and arginine; andR.sub.8 and R.sub.9 are the same or different and are selected from the group consisting of methionine, valine, alanine, phenylalanine, tyrosine, tryptophan, lysine, glycine, leucine and arginine;and further comprising at least one operational element selected from the group consisting of a promoter, an operator, a leader sequence, a Shine-Dalgarno sequence, a ribosome binding site and a terminator codon.