Patent ID: 8367802
Filing Date: 2013-02-05
Classification: C07K

Abstract:
1. A method of producing a selected desAB fibrin monomer, or fibrin II monomer in acetic acid solution, for subsequent polymerization and application as a fibrin sealant for hemorrhage, the method comprising the steps of: i. forming a selected desAB fibrin polymer, or fibrin II polymer, in a selected buffer solution by treatment of fibrinogen with thrombin in a dialysis tube; ii. dissolving the formed desAB fibrin polymer, or fibrin II polymer via dialysis against the acetic acid solution containing 0.125% acetic acid at about 4° C. to 8° C. so as to produce a selected desAB fibrin monomer in said acetic acid solution, thereby at least partially removing thrombin and plasminogen from the solution comprising the fibrin monomer and the acetic acid solution; and iii. adjusting the amount of desAB fibrin monomer in the acetic acid solution of pH about 3.5 at about 4° C. to a concentration generally within a range of about 10 mg/ml to 15 mg/ml, wherein the produced desAB fibrin monomer in said acetic acid solution has clotability over approximately 95%.