Patent ID: 6673590
Filing Date: 2004-01-06
Classification: A23G,A23K,A61K,A61Q,C11D,C12N,C12Y

Abstract:
A method of producing a subtilisin variant that is more effective than a precursor subtilisin in a low detergent concentration system having less than about 800 ppm detergent components present in the wash water, comprising:a) substituting an amino acid residue at one or more positions in a precursor subtilisin wherein the substitution alters the overall charge of the precursor subtilisin to make a subtilisin variant having a more negative charge or less positive charge compared to the precursor; b) testing the variant in a low detergent system having less than about 800 ppm detergent components present in the wash water by comparing the ability of the precursor and the variant to remove a stain wherein the effectiveness of the precursor is given a value of 1.0 and the variant with improved effectiveness achieves a value greater than 1.0; and c) producing said variant having improved effectiveness.