Patent ID: 9045803
Filing Date: 2015-06-02
Classification: C12Q

Abstract:
1. A method for detecting and analyzing the nucleotide sequence of a reverse transcriptase (RT) region of the polymerase (Pol) gene of Hepatitis B Virus (HBV), the method comprising: contacting a nucleic acid sample with a primer pair specific for a target RT region and carrying out a real-time amplification reaction to produce and quantify an amplified product if the target RT region is present in the sample; determining amount of amplified product produced and diluting the amplified product by 1:2 to 1:8 prior to DNA sequencing; determining the DNA sequence of the amplified product; and comparing the DNA sequence of the amplified product to: to determine the HBV genotype or serotype of the amplified product and/or the presence or absence of one or more DNA sequences characteristic of an HBV mutation that confers resistance to a drug or vaccine.