Patent ID: 7943330
Filing Date: 2011-05-17
Classification: G01N

Abstract:
1. A method of harvesting peptide fragments comprising: presenting an alkynyl-derivatized sugar to a cell; wherein the alkynyl-derivatized sugar has an alkynyl functional group; and wherein the cell is capable of producing a glycoprotein; incorporating the alkynyl-derivatized sugar into the cell; wherein the alkynyl-derivatized sugar is subsequently used by the cell to produce a tagged glycoprotein; and wherein the tagged glycoprotein includes a glycan portion, a peptide portion; and the alkynyl functional group; reacting the tagged glycoprotein with a probe to produce a labeled glycoprotein, wherein the labeled glycoprotein includes the glycan portion, the peptide portion, the alkynyl functional group and the probe; capturing the labeled glycoprotein onto a solid support, wherein the solid support is labeled with a binding moiety capable of binding to the probe of the labeled glycoprotein; and washing the solid support with an enzyme digestion to remove peptide fragments from the peptide portion of the labeled glycoprotein, resulting in the peptide fragments being harvested.