Patent ID: 8932817
Filing Date: 2015-01-13
Classification: C12Q

Abstract:
1. A method for detecting a target nucleic acid sequence in a sample comprising: (a) providing a sample suspected of containing a target nucleic acid; (b) generating from the target nucleic acid a defined ratio of at least two differentiable amplicon species, (c) detecting the presence and amount of each generated amplicon species, wherein the at least two amplification oligomers hybridizing to the complementary strand of the target nucleic acid hybridize to the same sequence on said strand, and one of the at least two amplification oligomers contains one or more nucleobase substitutions relative to the other; wherein the generating and detecting steps are each performed in a single vessel; and wherein the detecting step comprises hybridizing the at least two differentiable amplicon species with distinguishable probes that each hybridize to only one of the at least two differentiable amplicons.