Patent ID: 8383364
Filing Date: 2013-02-26
Classification: A61K,A61P,C12N

Abstract:
1. A method for inducibly expressing a nucleic acid sequence of interest, the method comprising: providing a nucleic acid construct comprising said nucleic acid sequence of interest operably linked to an inducible gene expression system that comprises a reverse tetracycline-controlled transactivator (rtTA) encoding nucleic acid sequence and/or a single chain rtTA encoding nucleic acid sequence, said rtTA encoding nucleic acid sequence and/or single chain rtTA encoding nucleic acid sequence comprising a mutation in a codon at rtTA amino acid position 9, and/or 19, and/or 37, and/or 56, and/or 67, and/or 68, and/or 138, and/or 157, and/or 171, and/or 177, and/or 195; introducing said nucleic acid construct to a suitable expression system; and allowing for inducible expression of said nucleic acid sequence of interest.