Patent ID: 7912652
Filing Date: 2011-03-22
Classification: C12Q,G01N,G16B

Abstract:
1. A method for identifying a putative mutation site within a target sequence of a sample comprising two or more discrete sequences, the method comprising: providing a mutational analysis module; collecting sequence information for the target sequence, comprising forward and reverse orientation sequence information, the forward orientation sequence information corresponding to a forward orientation sequence, and the reverse orientation sequence information corresponding to a reverse orientation sequence; scanning the forward orientation sequence information for a first mixed-base signature and the reverse orientation sequence information for a second mixed-base signature wherein the mixed-base signatures are derived from a selected locality of the target sequence; comparing the first mixed-based signature relative to the second mixed-base signature to generate a comparison; identifying a putative mutation site based on the comparison of the first mixed-base signature and the second mixed-base signature; and displaying the putative mutation site to a user, wherein the mutational analysis module comprises a hardware device and performs at least one of the comparing and the identifying.