Patent ID: 7691245
Filing Date: 2010-04-06
Classification: C07K,G01N

Abstract:
1. A method for controlling sample introduction in microcolumn separation techniques, in especially in capillary electrophoresis (CE), wherein an electrolyte buffer and a more or less concentrated sample are transported through a system of capillary channels, and wherein said sample consisting of components having different electrophoretic mobilities is injected as a sample plug into a sampling device, which comprises at least a channel for the electrolyte buffer and a supply and drain channel for said sample, said electrolyte channel and said supply and drain channels for said sample intersecting each other, and said supply and drain channels each being inclined to said electrolyte channel and discharging into it at respective supply and drain ports, wherein a distance between said supply port and said drain port geometrically defining a sample volume, characterized in that the injection of said sample plug into said electrolyte channel is accomplished electrokinetically by applying an electric field across said supply and drain channels for a time period which is at least long enough, that a slowest of said sample components having the lowest electrophoretic mobility is contained within said geometrically defined volume.