Patent ID: 8614085
Filing Date: 2013-12-24
Classification: C12P,Y02E

Abstract:
1. A recombinant yeast cell comprising: a) an isobutanol biosynthetic pathway, wherein the recombinant yeast cell comprises heterologous genes encoding an acetolactate synthase, an acetohydroxy acid isomeroreductase, an acetohydroxy acid dehydratase, a branched-chain keto acid decarboxylase, and a branched-chain alcohol dehydrogenase that perform the following substrate to product conversions: b) at least one genetic modification in an endogenous gene encoding a multidrug ATP-binding cassette transporter protein, wherein the multidrug ATP-binding cassette transporter protein has at least 95% sequence identity to SEQ ID NO:50, 52, 54, 56, or 58, wherein the at least one genetic modification decreases activity of the endogenous multidrug resistance ATP-binding cassette transporter protein, and;