Patent ID: 6812005
Filing Date: 2004-11-02
Classification: C12Q,Y10T

Abstract:
A method of detecting a first target sequence comprising a first target domain, a second adjacent target domain and a single stranded poly(A) sequence, said method comprising:a) hybridizing a first probe to said first target domain comprising: i) an upstream universal priming site (UUP); and ii)) a first target-specific sequence substantially complementary to said first target domain; b) hybridizing a second probe to said second probe to said second adjacent target domain comprising: iii) a second target-specific sequence substantially complementary to second adjacent target domain; iv) a downstream universal priming site (DUP); â€ƒwherein at least one of said first and second probe comprises at least a first adapter sequence;, said poly(A) sequence remains single-stranded, and wherein said target sequence and said first and second probes form a litigation complex; c) contacting said ligated complex with a ligase to form a ligated complex; d) contacting said ligated complex with a support having a poly(T) sequence, such that said single stranded poly(A) sequence hybridizes with said poly(T) sequence; e) removing unhybridized first and second probe sequence; f) denaturing said ligation complex; g) amplifying the ligated first and second probes to generate a plurality of amplicons; h) contacting said amplicons with an array of capture probes to form assay complexes; and detecting said assay complexes.