Patent ID: 9005933
Filing Date: 2015-04-14
Classification: C12Q

Abstract:
1. A method for amplifying a template nucleic acid, comprising: (a) providing a template nucleic acid, (b) contacting the template nucleic acid with a helicase, (c) hybridizing an oligonucleotide primer to the template nucleic acid, and (d) amplifying said template nucleic acid using the helicase dependent amplification (HDA) reaction, said HDA reaction introducing a recognition sequence into the template nucleic acid, said HDA reaction occurring in the presence of a nicking endonuclease that recognizes a nucleotide sequence of a completely or partially double-stranded nucleic acid molecule and cleaves only one strand of the template nucleic acid at a specific location relative to the recognition sequence, wherein the HDA reaction is a thermophilic HDA (tHDA), and wherein the template nucleic acid is shorter than 400 bp.