Patent ID: 7312074
Filing Date: 2007-12-25
Classification: C07K,C12N

Abstract:
1. A synthetic construct which is a peptide display carrier package (PDCP), said construct comprising a complex of a recombinant single-stranded polynucleotide and a chimeric protein, wherein i) the chimeric protein has ii) said recombinant polynucleotide comprises and wherein the nucleotide binding portion of the chimeric protein is bound to the nucleotide sequence motif of the recombinant polynucleotide, and wherein the chimeric protein-encoding portion of the recombinant polynucleotide is not bound by the nucleotide binding portion of the chimeric protein, and wherein the chimeric protein-encoding portion of the recombinant polynucleotide is protected from degradation by a binding moiety which is a viral protein and which is bound non-specifically to the polynucleotide irrespective of nucleotide sequence, and wherein said construct is produced in a host cell transformed with said recombinant polynucleotide and extruded therefrom without lysis of the host cell.