Patent ID: 8153426
Filing Date: 2012-04-10
Classification: A61K,A61P,C12N

Abstract:
1. A method for the preparation of efficient antigen-presenting human γδ T cells comprising: selecting γδ T cells out of human peripheral blood mononuclear cells, treating the selected cells with a stimulus for induction of antigen-presenting functions, and applying an antigen for uptake and presentation by these cells, the applied antigen being at least one pathogen-derived protein which is applied in the form of a purified DNA or RNA or a delivery vector containing such DNA or RNA and encoding it under conditions allowing endogenous expression of said pathogen-derived protein; wherein selecting γδ T cells is performed by culturing freshly isolated peripheral blood lymphocytes in the presence of structurally defined small molecular weight non-peptide compounds that induce the selective expansion of Vγ2Vδ2 further wherein the structurally defined small molecular weight non-peptide compounds are selected from at least one of isopentenyl pyrophosphate, 4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMB-PP), ethyl pyrophosphate (EPP), farnesyl pyrophosphate (FPP), dimethylallyl phosphate (DMAP), dimethylallyl pyrophosphate (DMAPP), ethyl-adenosine triphosphate (EPPPA), geranyl pyrophosphate (GPP), geranylgeranyl pyrophosphate (GGPP), isopentenyl-adenosine triphosphate (IPPPA), monoethyl phosphate (MEP), monoethyl pyrophosphate (MEPP), 3-formyl-1-butyl-pyrophosphate (TUBAg 1), X-pyrophosphate (TUBAg 2), 3-formyl-1-butyl-uridine triphosphate (TUBAg 3), 3-formyl-1-butyl-deoxythymidine triphosphate (TUBAg 4), monoethyl alkylamines, allyl pyrophosphate, crotoyl pyrophosphate, dimethylallyl-γ-uridine triphosphate, crotoyl-γ-uridine triphosphate, allyl-γ-uridine triphosphate, ethylamine, isobutylamine, sec-butylamine, iso-amylamine and nitrogen containing bisphosphonates.