Patent ID: 7087415
Filing Date: 2006-08-08
Classification: C12N,Y02P

Abstract:
1. A method for generating a population of variant DNA molecules in bacterial cells, said method comprising: (a) transferring a donor vector into a bacterial cell capable of homologous recombination, wherein wherein said first donor DNA sequence is homologous to said first target DNA sequence, and said second donor DNA sequence is homologous to said second target DNA sequence, wherein at least one of (1) the first donor DNA sequence and the first target DNA sequence are not identical to each other, or (2) the second donor DNA sequence and the second target DNA sequence, are not identical to each other; and (b) selecting for a population of bacterial cells within which homologous recombination between the donor vector and the target vector has occurred, such that the cells do not contain the negatively selectable marker, thereby generating a population of variant DNA molecules in bacterial cells.