Patent ID: 7417178
Filing Date: 2008-08-26
Classification: A23K,A23L,A23V,B82Y,C07K,C12N,G01N

Abstract:
1. A method of producing a recombinant milk protein in monocot plant seeds comprising the steps: (a) obtaining a monocot plant stably transformed with a chimeric gene having (i) a seed maturation-specific promoter, (ii) operably linked to said promoter, a leader DNA sequence encoding a monocot seed-specific transit sequence capable of targeting a linked polypeptide to an endosperm-cell organelle, wherein the promoter operably linked to the leader sequence comprises either SEQ ID NO:13 or SEQ ID NO:14 and (iii) a protein-coding sequence encoding a protein normally present in human milk, (b) cultivating the plant under seed-maturation conditions, and (c) harvesting the seeds from the plant, wherein the milk protein constitutes at least 3% of the total soluble protein contained in the harvested seeds.