Patent ID: 8212111
Filing Date: 2012-07-03
Classification: C07K,C12N

Abstract:
1. A method of producing a transgenic plant having an increased level of total fatty acids in a seed of the transgenic plant relative to a seed of a corresponding wild type plant comprising: i) transforming a plant cell with an expression vector comprising a lipid metabolism protein (LMP) nucleic acid and ii) generating from the plant cell the transgenic plant that expresses the LMP nucleic acid and that has an increased level of total fatty acids in the seed of the transgenic plant, wherein the LMP nucleic acid comprises a nucleic acid sequence selected from the group consisting of: a) the isolated nucleic acid sequence of SEQ ID NO: 27; b) an isolated nucleic acid encoding the polypeptide of SEQ ID NO: 28; c) an isolated nucleic acid that hybridizes under stringent conditions to the full-length nucleic acid sequence of SEQ ID NO: 27 or to a nucleic acid encoding the polypeptide of SEQ ID NO: 28, wherein the stringent conditions comprise hybridization in a 6× sodium chloride/sodium citrate (SSC) solution at about 45° C., followed by at least one wash in a 0.2×SSC, 0.1% sodium dodecyl sulfate (SDS) solution at 65° C.; d) an isolated nucleic acid having at least 95% sequence identity with the isolated full-length nucleic acid sequence of SEQ ID NO: 27 or with the nucleic acid encoding the polypeptide of SEQ ID NO: 28; and e) a nucleic acid encoding a polypeptide comprising an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 28.