Patent ID: 6139735
Filing Date: 2000-10-31
Classification: B01D,B01J,G01N

Abstract:
An artificial membrane support having at least one solubilized cellular receptor non-covalently immobilized thereon, wherein said immobilized cellular receptor is immobilized such that its tertiary structure after immobilization permits specific binding to ligands that are bound by said at least one cellular receptor when it is comprised in its native cellular environment, and wherein said artificial membrane support is contained in a liquid flow system; and further wherein said artificial membrane support is produced by the following steps:(i) obtaining an immobilized artificial membrane (IAM) liquid chromatographic (LC) stationary phase comprising a phospholipid monolayer;(ii) contacting said IAM LC stationary phase with at least one solubilized receptor under conditions wherein the at least one solubilized receptor becomes non-covalently immobilized in the phospholipid monolayer of said IAM LC stationary phase such that the tertiary structure of said at least one immobilized cellular receptor permits specific binding to ligands that are bound by said at least one cellular receptor when it is comprised in its native cellular environment; and(iii) exposing said artificial membrane support comprising at least one non-covalent immobilized cellular receptor to a liquid flow system.