Patent ID: 7226776
Filing Date: 2007-06-05
Classification: C12N,C12P,Y02E,Y02P

Abstract:
1. A recombinant host cell comprising: a first heterologous polynucleotide segment comprising a sequence encoding a polysaccharase under the transcriptional control of a surrogate promoter comprising the polynucleotide sequence of SEQ ID NO: 1 or 2, or a fragment thereof, wherein said promoter causes increased expression of said polysaccharase as compared to expression of said polysaccharase under transcriptional control of its native promoter; and a second heterologous polynucleotide segment comprising a sequence encoding a secretory polypeptide, wherein expression of said first and second polynucleotide segments results in increased production of said polysaccharase by the recombinant host cell as compared to production of said polysaccharase under transcriptional control of its native promoter and in the absence of expression of said second polynucleotide segment.