Patent ID: 9157905
Filing Date: 2015-10-13
Classification: C12N,C12Q,G01N

Abstract:
1. A method for determining whether a test gene is an oncogene, comprising: (a) transfecting a non-small cell lung cancer (NSCLC) cell line in a culture medium with an expression vector encoding at least one test gene, wherein the transfected NSCLC cell line expresses the test gene; (b) culturing the transfected NSCLC cell line under a condition which inhibits an expression or function of an EGFR oncogene or a PDGFRα oncogene that is (1) inherent to the NSCLC cell line and (2) required for proliferation of the NSCLC cell line; and (c) confirming whether or not the cultured NSCLC cell line proliferates, wherein when the cultured NSCLC cell line proliferates the test gene is determined to be a an oncogene and wherein when the cultured NSCLC cell line does not proliferate the test gene is determined not to be an oncogene.