Patent ID: 6372497
Filing Date: 2002-04-16
Classification: C07K,C12N,C12Q,C12Y,C40B,H01L

Abstract:
A method of producing a recombined polynucleotide having a desired characteristic, comprising:(a) providing a plurality of related-sequence double-stranded template polynucleotides, comprising polynucleotides with non-identical sequences; (b) providing a plurality of single-stranded nucleic acid fragments capable of hybridizing to the template polynucleotides; (c) hybridizing single-stranded nucleic acid fragments to the template polynucleotides and extending the hybridized fragments on the template polynucleotides with a polymerase, thereby forming a plurality of sequence-recombined polynucleotides; (d) subjecting the sequence recombined polynucleotides of step (c) to at least one additional cycle of recombination to produce further sequence-recombined poly-nucleotides; and, (e) selecting or screening the further sequence-recombined polynucleotides for the desired characteristic.