Patent ID: 6333501
Filing Date: 2001-12-25
Classification: G01J,G01N

Abstract:
A computer-implemented method for performing spectral calibration, comprising:collecting data from monitoring an analytical separation of a plurality of spectrally-distinguishable molecular species;creating a data matrix from the collected data, each element in the data matrix representing a signal intensity at a particular time and over a particular range of light wavelengths or an approximated time-derivative of the signal intensity;identifying regions in the data matrix having spectral response characteristics of at least one of the molecular species by performing rank analysis on portions of the data matrix;determining a set of pure component spectral responses from the identified regions;grouping similar pure component spectral responses into clusters;determining a representative spectral response for each of the clusters; andcorrelating the representative spectral response for each of the clusters with one of the molecular species.