Patent ID: 6037160
Filing Date: 2000-03-14
Classification: A61K,A61P,C12N

Abstract:
An isolated nucleic acid molecule that hybridizes to a nucleic acid molecule having a nucleic acid sequence selected from the group consisting of SEQ ID NO:6, SEQ ID NO:9, SEQ ID NO:11, and SEQ ID NO:20 under hybridization and wash conditions which allow 30% or less base-pair mismatch, wherein such conditions are determined by a formula:T.sub.m =81.5.degree. C.+16.6 log M+0.41(% G+C)-500/n-0.61(% formamide), wherein T.sub.m represents the temperature at which two complementary nucleic acid molecule strands will disassociate, assuming 100% complementarity between the two strands, n represents the number of nucleotides in the shorter strand of the duplex being hybridized and log M represents the ionic strengths of the hybridization and wash solutions in moles/liter;wherein said wash is conducted at a temperature of T.sub.m minus 30.degree. C.; andwherein said nucleic acid molecule encodes an epoxide hydrolase.