Patent ID: 7118860
Filing Date: 2006-10-10
Classification: C12Q

Abstract:
1. A method of generating a signal indicative of the presence of a target nucleic acid in a sample, comprising forming a cleavage structure by incubating a sample comprising a target nucleic acid with a probe comprising a binding moiety and a secondary structure that changes upon binding of said probe to said target nucleic acid, wherein said binding moiety comprises a region of said probe that is released upon cleavage of said probe with a nuclease and binds to a capture element, and cleaving said cleavage structure with said nuclease at a cleaving temperature to release a nucleic acid fragment and generate a signal, wherein said secondary structure of said probe is stable at or below said cleaving temperature when not bound to said target nucleic acid, and generation of said signal is indicative of the presence of a target nucleic acid in said sample; and detecting and/or measuring said signal.