Patent ID: 6214340
Filing Date: 2001-04-10
Classification: A61K,C07K,C12N,C12P,C12R

Abstract:
A physiologically active substance sulphostin showing the following physicochemical properties:1) Appearance: white powder;2) Molecular weight: 272;3) Molecular formula: C.sub.5 H.sub.13 N.sub.4 O.sub.5 SP;4) Solubility: soluble in water and insoluble in lower alcohol, acetone, ethyl acetate, hexane and petroleum ether;5) Rf value measured by silica gel thin-layer chromatography: 0.28 with a developing solvent of n-butanol, acetic acid and water (2:1:1);6) Ultraviolet absorption spectrum: A terminal absorption is shown;7) Infrared absorption spectrum: when measured in a potassium bromide tablet, the following specific absorption bands (cm.sup.-1) are shown: 3510, 3355, 3250, 1672, 1658, 1624, 1545, 1365, 1308, 1240, 1188, 1130, 1064 and 922;8) Proton NMR spectrum: when measured in heavy water, the following signals .delta.(ppm) are shown: 4.18 (1H, dd, J=6.8, 12.0 Hz), 3.82 (1H, tdd, J=5.1, 7.3, 13.0 Hz), 3.70 (1H, tdd, J=5.1, 6.7, 13.0 Hz), 2.39-2.44 (1H, m), 2.10-2.28 (1H, m), and 1.89-2.03 (2H, m);9) Carbon 13 NMR spectrum: when measured in heavy water, the following signals .delta.(ppm) are shown: 20.5, 24.2, 45.4, 51.3 and 172.4;10) Phosphorus NMR spectrum: when measured in heavy water, the following signal .delta.(ppm) is shown: 6.01;11) Specific rotation: [.alpha.].sub.D.sup.28 -21.5.degree. (c 0.52, H.sub.2 O);12) Melting point: 203-208.degree. C. (decomposed);13) Color reaction: positive to ninhydrin reaction and Rydon-Smith reaction.