Patent ID: 8709817
Filing Date: 2014-04-29
Classification: A01N,C12N,C12Q,G01N,Y10T

Abstract:
1. A flow cytometry system for sorting a mixture of particles including particles having a characteristic A and particles having a characteristic B, the system including: a fluid delivery system for delivering a fluid containing the particles to a flow cytometry apparatus; a flow cytometry apparatus for receiving the fluid, forming the fluid into a stream and using flow cytometry to classify the particles according to the characteristics; and a fluid switching sorting system for sorting selected particles in the stream according to the classification and according to a sorting strategy to provide at least one population containing desired particles, wherein the fluid switching system comprises: a control having a microprocessor programmed with instructions for implementing a control strategy responsive to information received from the flow cytometry apparatus during sorting for controlling the fluid delivery system during sorting to vary a rate at which fluid is delivered as a function of at least one of the following: (1) a purity of the said at least one population with respect to either characteristic A particles or characteristic B particles; and (2) a quantity of characteristic A particles or characteristic B particles in the said at least one population relative to a total quantity of characteristic A particles or characteristic B particles in the stream.