Patent ID: 6291229
Filing Date: 2001-09-18
Classification: C12N,C12R

Abstract:
A pure bacterial culture useful for production of alkalitolerant enzymes wherein the bacteria consist of aerobic, Gram-positive, coccoid or short rod-shaped, obligate alkaliphilic bacteria consisting essentially of the following characteristics:a) cells are often found in pairs, exhibiting a characteristic snapping form of cell division so as to give a V-form arrangement;b) no growth below pH 8;c) on alkaline-agar, forms opaque, orange colored, punctiform or circular colonies;d) in alkaline-broth, growth (37.degree. C.) is flocculent, with the formation of a sediment and surface ring;e) grows optimally at above 30.degree. C., no growth above 40.degree. C.;f) grows at NaCl concentration of between 0-10%, no growth at a concentration of 12% NaCl;g) grows on yeast extract, peptones and carbohydrates;h) gives a positive response to the catalase reaction test;i) gives a negative response to the following tests:1) KOH2) Aminopeptidase3) Oxidase4) Gelatin hydrolysis5) Starch hydrolysis.