Patent ID: 8697380
Filing Date: 2014-04-15
Classification: G01N

Abstract:
1. A method for selecting compounds having a modulating effect on the activation state of a dimer of VFT-domain proteins expressed in cell membranes present in a measuring medium, said dimer consisting of a first protein and a second protein, each of the first protein and the second protein having a VFT domain, said proteins being identical or different, wherein the method comprises the following steps: (a) labeling the first protein in the N-terminal portion of the VFT domain thereof with a member of a pair of FRET partners; (b) labeling the second protein in the N-terminal portion of the VFT domain thereof with the other member of said pair of FRET partners; (c) exciting the FRET partners and measuring a FRET signal in the absence and in the presence of a test compound: (d) selecting the test compound as a modulating compound if a difference in the FRET signal in the absence and in the presence of the test compound is measured in step (c), wherein the FRET signal measured in step (c) is within a time window in which the signals measured in the presence and in the absence of a reference agonist or antagonist compound are different.