Patent ID: 8841267
Filing Date: 2014-09-23
Classification: A61P,C12N,C12Q

Abstract:
1. A non-naturally occurring miR-196a-2 miRNA comprising a nucleic acid having a stem-loop structure wherein the stem-loop structure incorporates a miR-196a-2 scaffold and a mature strand and star strand duplex, wherein the sequence of said mature strand of the duplex is distinct from the sequence of the endogenous mature strand of any miRNA and is either: (i) 100% complementary to a portion of a target RNA; or (ii) 100% complementary to a portion of the target RNA except that the target RNA is capable of forming a wobble base pair with position 1 of the mature strand, and wherein said star strand is complementary to said mature strand except that position 1 of the mature strand is U and the opposite nucleotide in the star strand is G and the duplex contains a mismatch at position 12 of the mature strand and either no additional mismatches or wobble pairs, or mismatches or wobble pairs at one or more of positions 5, 18, 19, 20, 21, 22, or 23 and no mismatches or wobble pairs at any of positions 2, 3, 4, 6-11 and 13-17 of the mature strand of the duplex, wherein positions of the mature strand are numbered relative to the 5′ end of the mature strand and wherein said mature strand of the duplex is between about 19 and 25 nucleotides in length.