Patent ID: 8735558
Filing Date: 2014-05-27
Classification: A61K,C07C,C07H

Abstract:
1. A method for modulating the secretion, expression or synthesis of adhesion proteins or angiopoietin in cells, comprising contacting said cells with an effective amount of an isolated, purified or synthesized compound, or its salt or ester thereof, selected from the formula: wherein R R R wherein at least two of R R R wherein the secretion of adhesion proteins is determined by a method comprising the following steps: 1. Growing cells in RPMI 1640 medium over night before drug-treatment; 2. Replacing cells cultures with fresh RPMI medium containing selected compound (1, 5, or 10 ug/ml concentration) or DMSO (as control) at 0 hour; 3. Replacing with fresh culture medium without drug at 4 hours (A) or 8 hours (B); 4. Determining adhesion proteins in aliquot of culture medium at 2, 4, 8 and 24 hour; 5. Determining adhesion proteins by Western blot with monoclonal antibody specific only to human adhesion proteins; 6. Comparing the adhesion proteins in control and drug-treated cells before drug removal at 4 and 8 hours; 7. Checking cells morphology at all times to make sure cells are alive; 8. Comparing the reduction of adhesion proteins in treated cells (compare to control cells) at 4, 8 and 24 hours; and 9. Comparing the amount of adhesion proteins secreted by treated cells at 24 hours (including those have 4 and 8 hours of drug-treatments), wherein the secretion continues after removal of drug, indicating that the cells are alive after drug treatment and capable of secreting adhesion proteins.