Patent ID: 6569998
Filing Date: 2003-05-27
Classification: A61K,C07H,C07K

Abstract:
A process for making a glycosulfopeptide compound, comprising:(a) providing a presynthesized peptide comprising at least one tyrosine residue and at least one amino acid residue having a GalNAc side group linked thereto via an N- or O-linkage, respectively; (b) providing a purified core 1 &bgr;1,3-galactosyltransferase and enzymatically linking a Gal to the GalNAc via the core 1 &bgr;1,3-galactosyltransferase; (c) enzymatically linking a GlcNAc to the GalNAc via a N-acetylglucosaminyltransferase; (d) enzymatically linking a second Gal to the GlcNAc via a &bgr;1,4-galactosyltransferase; (e) enzymatically linking a sialic acid to the second Gal via an &agr;2,3-sialyltransferase; (f) enzymatically linking a Fuc to the GlcNAc via an &agr;1,3-fucosyltransferase; and (g) enzymatically linking a sulfate to the tyrosine residue via a tyrosylprotein sulfotransferase.