Patent ID: 7098033
Filing Date: 2006-08-29
Classification: C12N

Abstract:
1. A fast transformation method performed by an automatic transformation apparatus, comprising: thawing competent cells, prepared from mixing the competent cells with a plasmid DNA in the container to form a mixture by using a automatic micropipette to pipet the plasmid DNA into the container; transferring the container by a robot arm to a second tank heated by a temperature control unit for about 0–180 seconds whereby the mixture in the container is subjected to a heat shock treatment; providing a plate filled with a selective medium at about 0° C. to less than room temperature by a plate-loading unit; providing a plating tool of about −90° C. to less than room temperature on the selective medium by a plating unit; moving the plating tool on the selective medium to spread the mixture thereon by the plating unit without recovering the competent cells in a cultured medium; and culturing the mixture on the selective medium in an incubating unit to obtain the transformed cells.