Patent ID: 7812148
Filing Date: 2010-10-12
Classification: A01K,A61K,C12N

Abstract:
1. An isolated vector comprising polynucleotide, wherein the polynucleotide comprises: a. an extended methylation-free CpG island encompassing dual, divergently transcribed promoters, wherein the CpG island b. an expressible nucleic acid terminated by a polyadenylation signal, and c. a selectable marker gene operably linked to a promoter, wherein the selectable marker gene is an antibiotic resistance gene, wherein when the vector is linearized and integrated into a chromosome, both the extended methylation-free CpG island and the selectable marker gene are operably linked to the expressible nucleic acid, and the components are positioned in the order: extended methylation-free island, expressible nucleic acid, selectable marker gene, in the 5′ to 3′ orientation with respect to the sense strand of the expressible nucleic acid, and wherein the polyadenylation signal at the 3′ end of the expressible nucleic acid is within 2000 by of the proximal end of the selectable marker gene, and wherein the expression of the expressible nucleic acid is increased relative to the expression in the presence of the extended methylation-free CpG island alone or when the selectable marker is 5′ of the expressible nucleic acid.