Patent ID: 6077951
Filing Date: 2000-06-20
Classification: C07H,C07K,C09B

Abstract:
A method to obtain a glycopeptide or glycoprotein derivative comprising subjecting a glycopeptide or glycoprotein to solid-phase Edman degradation to form the glycoamino acid derivative followed by separating and characterizing the glycoamino acid derivative; wherein prior to subjecting the glycopeptide or glycoprotein to Edman degradation, the glycopeptide or glycoprotein is treated so as either to prevent binding of free carboxyl groups of the glycopeptide or glycoprotein to immobilized amine groups on a solid support associated with the solid-phase Edman degradation, or to prevent hydrolysis of terminal sialic acids present on the glycopeptide or glycoprotein.