Patent ID: 6030845
Filing Date: 2000-02-29
Classification: G01N

Abstract:
An immunoassay method in which whole blood is lysed and antigens in the sample of whole blood are reacted in an agglutination reaction with insoluble carriers onto which antibodies specifically reacting with the antigens in the sample have been immobilized,comprising the steps of:lysing the whole blood with a hemolysis reagent;irradiating the resulting agglutination mixture with light including a wavelength substantially free from absorption by hemoglobin and the hemolysis reagent; andmeasuring the resulting agglutinating mixture to determine a change in absorbance by the irradiation of the reacted agglutination mixture with the light of the wavelength substantially free from absorption of the hemoglobin and the hemolysis reagent, where said sample is whole blood and the whole blood is lysed at or before the agglutination reaction.