Patent ID: 7822556
Filing Date: 2010-10-26
Classification: G16B

Abstract:
1. A method for comparing gene expression data between a control sample and an experimental sample, comprising (a) furnishing a control array and an experimental array, wherein the control and experimental arrays have a common set of primers for detecting a set of genes of interest; (b) performing real-time polymerase chain reaction (RT-PCR) analysis for the control sample using the control array to generate a control expression dataset, (c) performing RT-PCR analysis for the experimental sample using the experimental array to generate an experimental expression dataset, (d) filtering each of the control and experimental datasets to identify, based on the RT-PCR data, a set of data genes and a set of normalizer genes, wherein (e) normalizing each of the control and experimental datasets, comprising: (f) comparing the control difference value of a data gene-normalizer gene pair with the experimental difference value of the corresponding data gene-normalizer gene pair, wherein a change in the difference value indicates that the expression levels of the data gene may be different in the control sample as compared to the expression level of the same data gene in the experimental sample; (g) ranking the set of data genes based on the comparisons of difference values in step (f); and (h) outputting at least one of the ranked list and information based on the ranked list in a user readable format.