Patent ID: 7691564
Filing Date: 2010-04-06
Classification: C07K,C12N,G01N

Abstract:
1. A method for detecting G protein coupled receptor signaling in the presence of a test sample, the method comprising: contacting a eukaryotic cell selected from the group consisting of bovine, human, murine, rat, monitoring fluorescence resonance energy transfer (FRET) or bioluminescence resonance energy transfer (BRET) in said cell, wherein the eukaryotic cell comprises: wherein the cell expresses a G protein coupled receptor and said first and second fusion proteins and a β or γ subunit of the heterotrimeric G protein which is not part of the first or second fusion protein, wherein said first and second fusion proteins and said β or γ subunit of the heterotrimeric G protein which is not part of the first or second fusion protein form a heterotrimeric G protein comprising an α, β, and γ subunit, wherein the heterotrimeric G protein so formed is capable of binding to said G protein coupled receptor and is capable of FRET or BRET between said first and second fusion proteins; observing a change in FRET or BRET between said first and second fluorescent or luminescent protein moieties upon contact with the test sample, which suggests that a component in the test sample binds to and activates the G protein coupled receptor expressed in the cell.