Patent ID: 8934700
Filing Date: 2015-01-13
Classification: B01L,C12M,C12N,G02B,G06T,H04N

Abstract:
1. An apparatus for isolating individual target cells, the apparatus comprising: a body structure comprising a main channel, a collection channel, and a waste channel fluidly coupled at a first fluid junction, the body structure further comprising a plurality of trapping channels intersecting the collection channel, each of the trapping channels having a diameter at a location adjacent to the intersection of the trapping channel with the collection channel that is less than a diameter of an individual target cell, the diameter of the trapping channel at the location adjacent to the intersection of the trapping channel with the collection channel varying between a first diameter and a second diameter, the body structure further comprising a tubular member extending from the body structure, the tubular member being in fluid communication with one or more of the plurality of trapping channels; an imaging system configured to image each of a plurality of individual target and non-target cells within the main channel, thereby producing imaging data; a processor configured to perform real-time, multivariate analyses of the imaging data; a directing system configured to direct the individual target cells; and a pressure source in fluid communication with one or more of the collection channel, the waste channel, the first side channel, and the second side channel.