Patent ID: 7534587
Filing Date: 2009-05-19
Classification: C12Q

Abstract:
1. A method of genotyping plants of the species Triticum aestivum and the genus Triticeae at a microsatellite locus, the method comprising a) amplifying chromosomal DNA with one or more oligonucleotide primer pairs specifically hybridizing to said locus of a region of said chromosomal DNA, wherein said region of the DNA comprises a repeated motif to obtain an amplification product, b) wherein each primer pair consists of a first oligonucleotide of SEQ ID NO. x and a second oligonucleotide of SEQ ID NO. x+1, and wherein x=1, 3, 5, 7, 9, 11, 13, 15, 17, and/or 19; and c) size fractionating the amplification product to provide a measure of the said motif of the chromosomal DNA between said primer pairs, wherein the size of the amplification product is polymorphic for said locus and provides a marker for genotyping said plants.