Patent ID: 8043830
Filing Date: 2011-10-25
Classification: C12P

Abstract:
1. A method of producing a biotinylated antibody, the method comprising the steps of: (a) expressing an antibody having a biotinylation acceptor in a eukaryotic cell, wherein the biotinylated acceptor sequence is at the N- or C-terminus of the antibody and is attached to the antibody by a linker sequence wherein the linker sequence is SEQ ID NO:17 or SEQ ID NO:18, and wherein the antibody further comprises a eukaryotic leader sequence for extracellular secretion; (b) expressing in the eukaryotic cell a heterologous biotin protein ligase that is retained in the eukaryotic cell endoplasmic reticulum (ER), wherein the heterologous biotin protein ligase biotinylates the antibody forming a biotinylated antibody; and wherein, the biotinylated antibody is secreted from the eukaryotic cell into a culture medium and the biotinylated antibody retains its capacity to bind antigen, and (c) purifying the biotinylated antibody from the culture medium.