Patent ID: 8445195
Filing Date: 2013-05-21
Classification: C12Q,G01N

Abstract:
1. A method for the simultaneous detection of bacterial species that cause zoonosis belonging to the genera Anaplasma, Ehrlichia, Bartonella, Borrelia, Coxiella, Francisella and Rickettsia comprising the following steps: (a) placing a sample for analysis in contact with a reaction mixture containing specific primers SEQ ID NOs: 1, 2, 7, 8, 25, 26, 28, 29, 31, 32, 36 and 37, to perform multiplex polymerase chain reaction; (b) amplifying the sequences by means of polymerase chain reaction: and (c) detecting and identifying the amplification products formed in the previous step, said detection information being indicative of the presence or absence of zoonosis-causing bacteria.