Patent ID: 7858771
Filing Date: 2010-12-28
Classification: A61K,A61P,C12N

Abstract:
1. A chemically modified short interfering nucleic acid molecule (siNA), wherein: (a) the siNA molecule comprises a sense strand and a separate antisense strand, each strand having one or more pyrimidine nucleotides and one or more purine nucleotides; (b) each strand is independently 18 to 27 nucleotides in length, and together comprise a duplex having between 18 and 23 base pairs; (c) the antisense strand is complementary to a human cholinergic receptor muscarinic 3 (CHRM3) RNA sequence comprising SEQ ID NO: 305; (d) each pyrimidine nucleotide of the sense strand has a 2′-fluoro modification, each purine nucleotide of the sense strand has a 2′-deoxy modification, and both the 5′- and 3′-ends of the sense strand have a terminal cap moiety; (e) each pyrimidine nucleotide of the antisense strand has a 2′-fluoro modification, each purine nucleotide of the antisense strand has a 2′-deoxy modification, and the antisense strand has a phosphorothioate internucleotide linkage at the 3′-end; and, (f) wherein the siNA molecule mediates RNA interference.