Patent ID: 6074875
Filing Date: 2000-06-13
Classification: A61P,C07K,C12N

Abstract:
A method of producing a cell line, the cell line expressing a desired polypeptide in response to extracellular gastric inhibitory polypeptide (GIP; glucose-dependent insulinotropic polypeptide) and/or glucagon-like peptide-1 (GLP-1; insulinotropin) hormones; the method comprising the steps of:(a) selecting a cell line that does not express the GIP or GLP-1 receptor; and(b) transfecting the first cell line of step (a) with nucleic acid encoding GIP and/or GLP-1 receptors, and nucleic acid encoding a desired polypeptide, the nucleic acid encoding the polypeptide being under the control of a promoter containing regulatory elements responsive to a signal transmitted by the receptor(s) caused by binding of GIP or GLP-1 wherein transcription of the nucleic acid encoding the desired polypeptide is modulated by GIP and/or GLP-1 binding to the receptor(s).