Patent ID: 7150974
Filing Date: 2006-12-19
Classification: C07K,G01N

Abstract:
1. A method for identifying a compound that inhibits binding of a parathyroid hormone to a parathyroid hormone receptor by competitively binding to the parathyroid hormone receptor, the method comprising: (a) providing a recombinant polypeptide that: (i) comprises an amino acid sequence that is at least 30% identical to SEQ ID NO:20, and (ii) binds to parathyroid hormone; (b) contacting the polypeptide with a parathyroid hormone, or a parathyroid hormone receptor-binding fragment thereof, in the presence of a candidate compound; and (c) comparing the level of binding of the polypeptide to the parathyroid hormone or fragment thereof in the presence of the candidate compound with the level of binding of the polypeptide to the parathyroid hormone or fragment thereof in the absence of the candidate compound, wherein a lower level of binding in the presence of the candidate compound than in its absence indicates that the candidate compound competes with parathyroid hormone for binding to the receptor.