Patent ID: 8642755
Filing Date: 2014-02-04
Classification: C07H

Abstract:
1. A method of sulfurizing at least one phosphite or thiophosphite linkage in an oligonucleotide, said method comprising: combining a defined amount of phenylacetyl disulfide and a defined amount of phenylthioacetic acid, with a solvent to produce a composition that comprises at least 1% phenylthioacetic acid by volume and at least 0.1 M phenylacetyl disulfide; and contacting said at least one phosphite or thiophosphite with said composition for a time sufficient to convert said at least one phosphite or thiophosphite to a phosphorothioate or phosphorodithioate internucleotide linkage, wherein said composition is used in said contacting step immediately after preparing said composition.