Patent ID: 6291189
Filing Date: 2001-09-18
Classification: G01N,Y10T

Abstract:
A method of determining, at a resolution of about 1-5 amino acid residues, the position of a peptide amide group labeled with a heavy hydrogen in a protein of known amino acid sequence, said method comprising the steps of:(a) progressively degrading, under conditions of slow hydrogen exchange, said protein into at least one series of subfragments, wherein each subfragment in each of said series is shorter than the preceding subfragment in the series by about 1-5 amino acid residues at one terminus; and(b) quantifying the amount of heavy hydrogen label on each subfragment; and(c) correlating the amount of heavy hydrogen label of each subfragment with the amino acid sequence of the protein, thereby localizing the position of the labeled peptide amide group in the protein to within about 1-5 amino acid residues.