Patent ID: 6482655
Filing Date: 2002-11-19
Classification: C12Q,G01N,Y10S

Abstract:
An immunoassay procedure for detecting and quantifying an unknown antibody in bulk solution comprising:(a) placing in the bulk solution a conjugate comprising: a peptide epitope recognized by the unknown antibody, said peptide epitope having bonded thereto a pair of fluorescent materials, the peptide epitope of said conjugate capable of moving between a more folded configuration to exhibit fluorescent quenching of said conjugate and a more spaced configuration to exhibit fluorescence of said conjugate; and (b) adding the unknown antibody to the bulk solution to bind with the peptide epitope of said conjugate to alter the configuration of the pair of fluorescent materials from the more folded configuration to the more spaced configuration thus altering the fluorescence of said pair of fluorescent materials to detect and quantify the unknown antibody.