Patent ID: 8431693
Filing Date: 2013-04-30
Classification: C07H,Y02P

Abstract:
1. A process comprising the steps of: a) synthesizing a nucleic acid molecule comprising one or more nucleotides, using a method selected from the group consisting of solid phase phosphoramidite, solution phase phosphoramidite, solid phase H-phosphonate, solution phase H-phosphonate, hybrid phase phosphoramidite, and hybrid phase H-phosphonate-based synthetic methods; b) contacting said nucleic acid molecule from step (a) with aqueous alkylamine; ammonia; a low-volatility amino compound selected from the group consisting of polyamine, PEHA, PEG-NH c) contacting reaction mixture having said nucleic acid molecule from step (b) with pyridine-HF, DMAP-HF (dimethylaminopyridine-HF), urea-HF, TASF (tris(dimethylamino)sulfonium difluorotrimethylsilane), DAST (diethylaminosulfur trifluoride), polyvinyl pyridine-HF or an amine-HF reagent of formula AA and a polar solvent selected from the group consisting of DMSO, DMF, ethanol, isopropanol, methanol, acetonitrile, and combinations thereof, under aqueous conditions for the removal of a silyl protecting group and/or a 2′-OH protecting group, wherein R R R n is 1 to 20; d) loading reaction mixture having said nucleic acid molecule from step (c) onto a chromatography media in a suitable buffer; and e) applying a purification gradient using a suitable elution buffer, and pooling and desalting the pure fractions.