Patent ID: 6825399
Filing Date: 2004-11-30
Classification: C12N

Abstract:
An isolated DNA molecule encoding a functional eukaryotic acetohydroxy-acid synthase (AHAS) small subunit protein, wherein said DNA molecule hybridizes to a DNA molecule comprising a sequence having the complement of SEQ ID NO: 1 under conditions comprising:(a) hybridization at 42Â° C. for 20 hours in a solution comprising 50% formamide, 2Ã—SSC, 5Ã—Denhardt's solution, 1% sodium dodecyl sulfate (SDS), 0.05 mg/ml denatured salmon sperm DNA, and 0.05% NaPPi; (b) two washes at room temperature for 10 minutes in a solution comprising 0.4Ã—SSC and 0.1% SDS; and (c) one wash at 65Â° C. for 30 minutes in a solution composing 0.2Ã—SSC and 0.1% SDS.