Patent ID: 9149515
Filing Date: 2015-10-06
Classification: A61K,A61P,C12N

Abstract:
1. An in vitro method for detecting the presence or absence of CD11b −/lo , CD172 + (Sirpα), CD45RA − , CD24 int dendritic cells producing interferon-alpha (“IFN-α”), comprising the steps of: (a) separating bone marrow cells from plasmacytoid dendritic cells and conventional dendritic cells by removing CD45RA (b) incubating the separated bone marrow cells with at least one of a toll-like receptor-3 (“TLR3”) ligand, a toll-like receptor-7 (“TLR7”) ligand, and a toll-like receptor-9 (“TLR9”) ligand; (c) detecting whether IFN-α is produced; and (d) if IFN-α is produced, isolating or enriching CD11b