Patent ID: 6312896
Filing Date: 2001-11-06
Classification: C12Q

Abstract:
A method for assaying a sample for the presence of a nucleic acid binding protein, which comprises:a) mixing at least one predetermined single- or double-stranded nucleic acid containing at least one label and containing a protein binding nucleotide sequence with a sample which may contain a nucleic acid binding protein,b) incubating the mixture of step a) under conditions which allow the binding of said nucleic acid binding protein to said at least one predetermined single- or double-stranded nucleic acid to form a complex,c) adding a nucleic acid-cleaving enzyme or reagent to the mixture of step b),d) incubating the mixture of step c) under conditions which allow the cleavage of said at least one predetermined single- or double-stranded nucleic acid which has not formed a complex, ande) measuring the amount of said complex by means that do not include gel electrophoretic separation to measure said nucleic acid binding protein.