Patent ID: 6623923
Filing Date: 2003-09-23
Classification: A61K,C07K

Abstract:
A method for determining the presence of colon cancer in a patient, comprising the steps of:(a) obtaining a biological sample from the patient; (b) contacting the biological sample with an oligonucleotide, wherein said oligonucleotide is capable of hybridizing under moderately stringent conditions to a polynucleotide sequence selected from the group consisting of: (i) SEQ ID NO:12; and (ii) SEQ ID NO:13; â€ƒwherein said moderately stringent conditions include prewashing in a solution of 5Ã—SSC, 0.5% SDS, 1.0 mM EDTA (pH 8.0); hybridizing at 50Â° C.-65Â° C., 5Ã—SSC, overnight; followed by washing twice at 65Â° C. for 20 minutes with each of 2Ã—, 0.5Ã— and 0.2Ã—SSC containing 0.1% SDS; (c) detecting in the sample an amount of oligonucleotide that hybridizes to the polynucleotide; and (d) comparing the amount of oligonucleotide that hybridizes to the polynucleotide TO a predetermined cut-off value, wherein an increase in the amount of oligonucleotide that hybridizes to the polynucleotide as compared to the predetermined cut-off value indicates the presence of cancer in the patient.