Patent ID: 7689366
Filing Date: 2010-03-30
Classification: C12Q,G16B

Abstract:
1. A method for screening a plurality of bacterial plasmid DNA sequences comprising: a) generating a library comprising a plurality of bacterial plasmid DNA sequences, wherein said bacterial plasmid DNA sequences are cloned from isolated plasmid DNA; b) selecting one or more plasmid DNA sequences from said library; c) obtaining a partial sequence for at least a first bacterial plasmid DNA sequence, wherein the length of said partial sequence is less than one-third of the length of the plasmid DNA sequence; d) determining if the plasmid DNA sequence is a novel sequence by using an algorithm which: e) obtaining the full sequence of any DNA sequence(s) characterized as novel in step (d); f) adding the nucleotide sequence(s) obtained in step (e) to said database in step (b) to generate an updated database; and, g) sequentially repeating steps (b)-(f) for one or more additional plasmid DNA sequences.