Patent ID: 8377681
Filing Date: 2013-02-19
Classification: C07K,C12N,C12P,C12Q,C12R

Abstract:
1. A method of evolving a cell to acquire a desired property, comprising: (i) providing a yeast cell population comprising genetically different yeast cells of different mating types; (ii) culturing the cells under conditions whereby the yeast cells mate to form progeny yeast cells; and (iii) screening or selecting the progeny cells for evolved progeny cells that have evolved toward acquisition of the desired functional property; (iv) culturing the evolved progeny yeast cells under conditions whereby the cells recombine by meiosis to form ascospores; (v) separating the ascospores; and (vi) repeating steps (i) through (v), wherein the separated ascospores are used as the population comprising genetically different yeast cells of different mating type provided in step (i).