Patent ID: 9086414
Filing Date: 2015-07-21
Classification: A61K,A61P,C12Q,G01N

Abstract:
1. A method for treating a human with a cancer of epithelial origin, who has been determined to be c-erbB2 negative by immunohistochemistry (IHC) or by fluorescence in situ hybridization (FISH), comprising administering an erbB2-directed treatment and therapy to the human, if the human has been identified for such treatment by a method comprising (a) measuring the level of phosphorylation of the c-erbB2 receptor in one or more diseased cells obtained from the cancerous epithelial tissue of the human using a quantitative immunoassay; (b) comparing the level of phosphorylation of the receptor in the one or more diseased cells to the level of phosphorylation of the receptor in one or more reference standards having a known phosphorylation level; and (c) determining that the human should be treated with erbB2-directed treatment and therapy if the level of phosphorylation of the receptor in the one or more diseased cells is greater than the cut-point value obtained by comparing a population average level of phosphorylation of the receptor in known erbB2-phosphorylated cancers to the level of phosphorylation of the receptor in the reference standards.