Patent ID: 8092990
Filing Date: 2012-01-10
Classification: C12Q,G01N

Abstract:
1. A method of determining the presence of a target microorganism in a sample, the method comprising: combining with the sample an amount of flagged phage capable of binding to an outside surface of the target microorganism to create a flag labeled target microorganism; providing conditions and an amount of time sufficient to allow the flagged phage to bind to the outside surface of the target microorganism; and assaying the phage exposed sample to detect the presence of the flag labeled microorganism, without detecting phage amplified within the target microorganism and, wherein presence of the flag bound to the outside surface of the target microorganism indicates presence of the microorganism, wherein the detecting the presence of the flag labeled microorganism is by concentrating the flag labeled microorganism to enhance the capacity to detect the flag labeled microorganisms, and wherein the concentrating of flag labeled microorganisms is on a flow strip device having one or more immobilization zones, wherein the flag labeled microorganisms are captured and concentrated within the one or more immobilization zones.