Patent ID: 8357638
Filing Date: 2013-01-22
Classification: B01J,C12N

Abstract:
1. Method of producing an oligonucleotide library comprising a plurality of oligonucleotides, each oligonucleotide in the library having at least one predetermined position, a randomisation codon selected from a defined group of codons, the codons within said defined group coding for different amino acids, said method comprising the steps of: (a) Providing one or more double-stranded starter oligonucleotides, wherein the starter oligonucleotides comprise a blunt end; (b) Providing a plurality of different double stranded randomisation oligonucleotides comprising: (c) Ligating each double-stranded starter oligonucleotide to a double-stranded randomisation oligonucleotide to form ligated oligonucleotides; (d) Amplifying the ligated oligonucleotides; (e) Digesting the ligated oligonucleotide with the restriction endonuclease to form a plurality of randomised double-stranded oligonucleotides, each of which comprise, at one end, a randomisation codon; and, (f) Using the randomised double-stranded oligonucleotides as starter oligonucleotides and repeating method steps (a) to (e) to produce a plurality of randomised double-stranded oligonucleotides, each comprising an additional randomisation codon;