Patent ID: 9128095
Filing Date: 2015-09-08
Classification: C12N,C12Y,G01N

Abstract:
1. A method of diagnosis or prognosis of lung cancer in a subject, the method comprising: providing a tissue of the subject; processing the tissue to isolate mRNAs contained in the tissue; generating cDNAs complementary to the isolated mRNAs; mixing the cDNAs with a forward rimer consisting of SEQ ID NO: 5 and a reverse primer consisting of SEQ ID NO: 6, which to provide a mixture; performing a qRT-PCR using the mixture and a PCR composition to amplify one or more cDNA sequences recognized by the forward and reverse primers; determining an expression level of glutaredoxin 3 in the tissue based on results of the qRT-PCR; and diagnosing or prognosing lung cancer when the expression level is higher than a reference value.