Patent ID: 6699661
Filing Date: 2004-03-02
Classification: C12Q

Abstract:
A nucleic acid probe for determining a concentration of a target nucleic acid, said probe being labeled with a fluorescent dye, wherein:said probe is labeled at an end portion thereof with said fluorescent dye, and said probe has a base sequence designed such that, when said probe is hybridized with said target nucleic acid, at least one G (guanine) base exists in a base sequence of said target nucleic acid at a position 1 to 3 bases apart from an end base portion where said probe and said target nucleic acid arc hybridized with each other; whereby said fluorescent dye is reduced in fluorescence emission when said probe is hybridized with said target nucleic acid, wherein said fluorescent dye is selected from the group consisting of 6-joe, BODIPY TMR, Alexa 488, and Alexa 532.