Patent ID: 6608172
Filing Date: 2003-08-19
Classification: C07K,Y10S

Abstract:
A method of separating IgY(&Dgr;Fc) from IgY, the method comprising:(a) providing a pool of antibodies that includes an IgY that has an Fc region and an IgY(&Dgr;Fc) that lacks an Fc region, the IgY and the IgY(&Dgr;Fc) both being raised by immunization of an anseriform bird with a specific antigen; (b) passing the pool over an antigen matrix that has the specific antigen immobilized thereto with a binding buffer having a pH value ranging from pH 4 to pH 7 and an ionic strength of less than 50 mM; and (c) washing the IgY but not the IgY(&Dgr;Fc) from the antigen matrix with the binding buffer, thereby separating the IgY(&Dgr;Fc) from the IgY.