Patent ID: 8852898
Filing Date: 2014-10-07
Classification: C12P

Abstract:
1. A method for preparing Escherichia coli ( E. coli ) producing L-threonine, using site-specific mutation, the method comprising: (a) disrupting lacI, metA, lysA and tdh in the (b) mutating ilvA, thrA and lysC in the (c) inducing substitution of a promoter of L-threonine operon or acetyl CoA synthetase with a promoter selected from the group consisting of trc promoter, tac promoter, T7 promoter, lac promoter and trp promoter in the (d) introducing a vector containing one or more genes selected from the group consisting of a gene encoding L-threonine operon, rhtC, rhtA and rhtB into the