Patent ID: 7452691
Filing Date: 2008-11-18
Classification: C12Q

Abstract:
1. A method for detecting a carbapenem-hydrolyzing enzyme produced by a test microorganism, comprising: a) admixing a test microorganism with an antibiotic for which inactivation is to be determined, a permeabilizing agent for the test microorganism present in a non-growth-inhibiting microorganism-permeabilizing effective amount, and a strain of microorganism with known susceptibility for said antibiotic, to form an assay culture; b) incubating said assay culture under appropriate conditions and for a time sufficient to detect said carbapenem-hydrolyzing enzyme; and c) determining if there is growth in the assay culture, whereby, significant growth of the strain of microorganism in the assay culture or a distortion in a zone of inhibition in the assay culture is indicative of production of carbapenem-hydrolyzing enzyme by the test microorganism.