Patent ID: 7314973
Filing Date: 2008-01-01
Classification: C12N

Abstract:
1. A method for producing oligosaccharides having a low degree of polymerization, comprising the steps of: (a) selecting a vegetable gene which encodes a fructosyltransferase enzyme which converts sucrose into an oligosaccharide having a low degree of polymerization; (b) linking the vegetable gene to suitable transcription-initiation and transcription-termination signals to provide an expression construct; (c) transforming a plant cell from a mutant plant having an altered starch or sucrose metabolism with the expression construct; (d) regenerating a transgenic plant from the transformed plant cell; (e) culturing the transgenic plant under conditions enabling the expression and activity of the fructosyltransferase enzyme; and (f) isolating the oligosaccharides from the transgenic plant.