Patent ID: 6051686
Filing Date: 2000-04-18
Classification: A61K,A61P,C07K

Abstract:
A method for the synthesis of a modified parathyroid hormone related polypeptide (PTHrP) of the formula:Ala Val Ser Glu Xaa.sup.5 Gln Leu Leu His Asp Xaa.sup.1 Gly Xaa.sup.13 Ser Ile Gln Asp Leu Xaa.sup.19 Arg Xaa.sup.21 Xaa.sup.22-31 Xaa.sup.32 Xaa.sup.33 Xaa.sup..div. Term,wherein:Xaa.sup.5 is His or Ala;Xaa.sup.11 and Xaa.sup.13 are independently Lys, Arg, or Leu;Xaa.sup.19 and Xaa.sup.21 are independently Ala or Arg;Xaa.sup.22-31 is selected from the group consisting of (SEQ ID NOS: 26, 27, 28, 29, and 30);Xaa.sup.32 is His or Lys;Xaa.sup.33 is Thr, Glu, or Ala;Xaa.sup.34 is Ala, hSer, Tyr, or Leu; andTerm is OH, NR.sub.2, lactone, or Gly Arg Arg, where R is H or (C.sub.1 -C.sub.4) alkyl; and te pharmaceutically acceptable salts thereof, which method comprises sequentially coupling protected amino acids, optionally on a resin, deprotecting, and purifying the polypeptide product.