Patent ID: 8652407
Filing Date: 2014-02-18
Classification: B01L,Y10T

Abstract:
1. A self-contained assay device for detecting analyte(s) in a specimen comprising: a first housing having a bottom and a raised wall; a button tower centrally secured to the bottom of the first housing, the button tower including: a membrane housing block fixedly fit in a position on the raised wall of the first housing and having an opening configured to receive a liquid specimen, the membrane housing block including a porous membrane on a bottom portion, a liquid inlet orifice in a position facing the button tower, and a liquid-tight seal surrounding the opening; an absorbent blotter located adjacent to the bottom of the first housing, the absorbent blotter having a raised portion located in a position under the membrane housing block; a blotter barrier plate located on a surface of the blotter opposite the bottom of the first housing; a second housing adapted to be fixedly fit in the first housing, the second housing including: when the button is depressed, the second housing is able to rotate relative to the membrane housing block thus causing the ram pin to engage one of the cam surfaces of the rim portion of the second housing to contain the ram pin until it reaches the second opening in one of the chambers thus causing the ram pin to thrust into the cylinder to move the cylinder and piston assembly, opposite the force of the coil-over spring, against the liquid inlet orifice on the membrane housing block thus moving the ball bearing to break the seal and allow the reagent or wash solution to escape into the membrane housing block and onto the membrane.