Patent ID: 8084433
Filing Date: 2011-12-27
Classification: A61P,C07K,C12N

Abstract:
1. A method of inhibiting replication of an infecting Dengue virus in a mammalian host cell, the method comprising administering to the infected host cell a virus-inhibitory amount of an antisense oligonucleotide analog compound characterized by: (i) a nuclease-resistant backbone, (ii) capable of uptake by mammalian host cells, (iii) containing between 15-40 nucleotide bases, (iv) a substantially uncharged backbone, composed of morpholino subunits linked by phosphorodiamidate intersubunit linkages, joining a morpholino nitrogen of one subunit to a 5′ exocyclic carbon of an adjacent subunit, and (v) having a targeting sequence of at least 15 subunits that is complementary to a region within the 5′-terminal 25 bases of SEQ ID NO:6, and by said administering, forming within the host cell, a heteroduplex structure (i) composed of the positive sense strand of the virus and the oligonucleotide compound, and (ii) characterized by a Tm of dissociation of at least 45° C. and disruption of a stem-loop secondary structure.