Patent ID: 6423233
Filing Date: 2002-07-23
Classification: B01D,B01J,C07K

Abstract:
A process for purifying cyclosporin A comprising:a first chromatographic step comprising loading a mixture including cyclosporin A onto a first silica gel column; eluting the first column with a first eluent comprising more than 50 vol % toluene to produce a first eluate containing purified cyclosporin A; a second chromatographic step comprising loading the first eluate onto a second silica gel column; eluting the second column with a second eluent comprising more than 50 vol % toluene to produce a second eluate containing purified cyclosporin A; and, optionally, repeating the second chromatographic step, wherein the column load of cyclosporin A on at least one column is about 20 wt %, or greater, of the mass of the silica gel absorbent.