Patent ID: 7608431
Filing Date: 2009-10-27
Classification: C07K,C12N

Abstract:
1. A process for recombinant production of erythropoietin (EPO) in a human embryonic retina cell that expresses at least an adenoviral E1A protein, wherein said EPO is produced at a concentration of at least 3000 eU/ml, and wherein said EPO as produced has an average sialic acid content per EPO molecule of at least 7, the process comprising: a) providing an immortalized human embryonic retina cell that expresses at least an adenoviral E1A protein, said cell further comprising a nucleic acid encoding a sialyltransferase under control of a heterologous promoter, and said cell further comprising nucleic acid encoding EPO under control of a heterologous promoter; b) culturing the cell in a serum-free culture medium in a fed-batch process, wherein at least once during said culturing according to needs of nutrients glucose and at least one medium component selected from the group consisting of glutamine, leucine, serine, isoleucine, arginine, methionine, and cystine are added depending on the requirements of the cells; and c) harvesting EPO from the culture medium, wherein the EPO is present in the culture medium at a concentration of at least 3000 eU/ml and wherein said EPO as produced has an average sialic acid content per EPO molecule of at least 7.