Patent ID: 8852435
Filing Date: 2014-10-07
Classification: B01D,C07K

Abstract:
1. A method for harvesting and purifying a target biological product at the end of a production cycle comprising: a) Expressing a target biological product in a bioreactor; b) Disposing in the bioreactor at the end of the production cycle, an appropriate length of a purification and separation treatment assembly (PASTA) comprising: a flexible porous tube filled with a binding resin, wherein the porous tube comprises a plurality of pores smaller in size than the size of the binding resin and wherein the porous tube is sealed at both ends to provide a pre-determined quantity of resin; c) Capturing the target biological product by mixing the contents of the bioreactor to allow substantially complete binding of the target biological product to the resin in the PASTA; d) Removing the contents of the bioreactor except the PASTA from the bioreactor; e) Eluting the target biological product from the PASTA held in the bioreactor by adding to the bioreactor a buffer capable of removing the binding of the target biological product from the resin in the PASTA.