Patent ID: 8124756
Filing Date: 2012-02-28
Classification: C07H,Y02P

Abstract:
1. A method of preparing 5′-amino linker oligonucleotides, comprising the steps of: introducing an amino linker having a protecting group at the 5′ terminus of an oligonucleotide; and removing the protecting group from the amino linker oligonucleotide by contacting with a mixture of acetic acid and trifluoroethanol (F wherein the linker is a carbon chain having 1 to 30 carbons or polyethylene glycol presented by (CH the protecting group is selected from the group consisting of monomethoxytrityl (MMT), 6-(4,4′-Dimethoxy-4″-methylsulfonyl-trityl) (DMS(O)MT), 2-(Biophenyl-4-yl)propan-2-yloxycarbonyl (Bpoc), and 2-(3,5-dimethoxyphenyl)propan-2-yloxycarbonyl (Ddz) groups.