Patent ID: 9090933
Filing Date: 2015-07-28
Classification: A61P,C12Q,G01N

Abstract:
1. A method of isolating mucus layer colonocytes from a stool sample comprising: (a) gently agitating the stool sample in a wash solution; (c) centrifuging the stool sample in the wash solution to form a cell pellet; (d) disrupting the cell pellet in a cell preservation solution to form a resuspended cell pellet; (e) filtering the resuspended cell pellet through a first strainer and collecting supernatant; (f) filtering the supernatant through a second strainer to retain mucus layer colonocytes; and (g) combining the mucus layer colonocytes with at least one mucolytic agent and at least one chelating agent to form a suspension of isolated mucus layer colonocytes.