Patent ID: 8980310
Filing Date: 2015-03-17
Classification: A61K,A61P

Abstract:
1. A process for manufacture of long circulating non-pegylated liposomes comprising: dissolving one or more phospholipids and one or more sterols in a solvent or mixture of solvents; wherein the one or more phospholipids is a saturated phosphatidylcholine selected from the group consisting of distearoyl phosphatidylcholine (DSPC), hydrogenated soya phosphatidyl-choline (HSPC) and mixtures thereof; removing the solvent or mixture of solvents and adding an aqueous hydration media to the phospholipids and sterols; or adding an aqueous hydration media to the phospholipids and sterols in the solution; and removing the solvent or mixture of solvents; wherein the aqueous hydration media consists essentially of ammonium sulfate and sucrose and the amount of the aqueous hydration media used is in the range of 10 to 35 ml for each mmole of the phospholipid present to form long circulating non-pegylated liposomes; and removing ammonium sulphate from extra liposomal hydration medium by dialysis using a sucrose-histidine buffer solution.