Patent ID: 6653066
Filing Date: 2003-11-25
Classification: G01N,Y10S

Abstract:
A device for detecting the presence or amount of HIV-1 or HIV-2 antibody in a biological sample, comprising:a sample absorber pad for application of said sample; a first matrix, said first matrix being in fluid communication with said sample absorber pad, said first matrix being impregnated with a first glycoprotein covalently linked to bovine serum albumin, said first glycoprotein being selected from the group consisting of HIV-1 glycoprotein 41 and HIV-2 glycoprotein 36, said glycoprotein covalently linked to bovine serum albumin being conjugated with gold particles having a diameter of between about 5 nm and about 50 nm; and a second matrix in fluid communication with the first matrix, said second matrix having immobilized thereon at a point spatially separated from the first matrix a second glycoprotein covalently linked to bovine serum albumin, said second glycoprotein being HIV-1 glycoprotein 41 when said first glycoprotein is HIV-1 glycoprotein 41 and being HIV-2 glycoprotein 36 when said first glycoprotein is HIV-2 glycoprotein 36, said second glycoprotein being immobilized on the second matrix; wherein the first glycoprotein is capable of moving from the first matrix to the second matrix and to said point spatially separated from the first matrix after application of the sample to the first zone.