Patent ID: 8916385
Filing Date: 2014-12-23
Classification: G01N,Y10T

Abstract:
1. A method for determining the amount of estrone in a human plasma or serum sample, said method comprising: (a) processing a human plasma or serum sample by one or more methods to generate a processed sample, said one or more methods comprising liquid chromatography; (b) ionizing estrone in said processed sample with atmospheric pressure chemical ionization (APCI) in negative ion mode to produce one or more ions detectable by mass spectrometry; and (c) detecting the amount of the estrone ion(s) by tandem mass spectrometry, wherein the detected estrone ion(s) comprise an estrone fragment ion with mass to charge ratio of 143.02±0.5; wherein the amount of the estrone ion(s) is related to the amount of estrone in said human plasma or serum sample; and wherein said method has a limit of quantitation less than or equal to 500 pg/mL.