Patent ID: 8951782
Filing Date: 2015-02-10
Classification: C12M,C12N

Abstract:
1. An apparatus for separating cells using magnetic force, the apparatus comprising: a separation channel portion including an upper substrate, and a lower substrate that is manufactured by hardening a mixed solution of ferromagnetic particles and polymer resin such that the ferromagnetic particles are distributed within the lower substrate, and provided with a flow path through which a cell fluid containing a plurality of cells having at least one of diamagnetic and paramagnetic properties passes through by being coupled to the lower side of the upper substrate; an inlet opening, a passage for introducing the cell fluid by communication with the separation channel portion, formed on the front end of the separation channel; a first outlet opening formed at an upper part of the rear end of the separation channel and discharging cells, separated and flowing in the upper part of the separation channel within the cell fluid; a second outlet opening formed at a lower part of the rear end of the separation channel and discharging cells flowing in the lower part of the separation channel; and a magnetic field controller that generates a magnetic field within the flow path so that the cells in the cell fluid flow within the flow path and are separated by height by the magnetic field, wherein the flow path comprises a microstructure in which a plurality of protrusions and a plurality of recesses interposed between the protrusions are formed in a repeated manner along the flow direction of the cell fluid and extend so that the protrusions and recesses intersect with the flow direction of the cell fluid in order to increase the gradient of the magnetic field, wherein the length of the protrusions and the length of the recesses differ depending on the flow direction of the cell fluid, and the ratio of the length of the protrusions to the length of the recesses is 1:2.