Patent ID: 6251623
Filing Date: 2001-06-26
Classification: C12Q,Y10S

Abstract:
A quick assay method for evaluating the activity of a plant-derived, asparagine residue-specific endoprotease, comprising measuring fluorescence generated by a fluorescence quenching substrate split by an asparagine residue-specific endoprotease, and the fluorescence quenching substrate comprising an oligopeptide having an amino acid sequence with at least one asparagine residue, the C-terminal side is other than an isoleucine residue, a leucine residue, or a valine residue, wherein the oligopeptide has a 7-methoxycoumarin-4-yl-acetyl group arranged on the N-terminal side and a 2,4-dinitrophenyl group arranged on the C-terminal side.