Patent ID: 6280979
Filing Date: 2001-08-28
Classification: C12P

Abstract:
A process for the production of D(-)-phenylglycine from D(-)-N-carbamoylphenylglycine by the preparation of D(-)-N-carbamoylphenylglycine from DL-5-phenylhydantoin, comprising the steps of:a)i) culturing the strain Pseudomonas sp. having Accession No. ATCC 55940 and deposited with the American Type Culture Collection (ATCC), Manassas, Va., having hydantoinase activity, in a medium for 16-20 hrs,ii) separating the cells by centrifugation,iii) treating the solid cells obtained in step ii) with DL-5-phenylhydantoin in a buffer at a pH range of 7-10.5, at a temperature in the range of 25-30.degree. C. for a period of 2-6 hrs.iv) acidifying the mixture to obtain solid D(-)-N-carbamoylphenylglycine, andv) separating the solid product obtained in step iv) by filtration;b)reacting sodium nitrite with D(-)-N-carbamoylphenylglycine in sulphuric acid having a concentration of 1N to 4N at a temperature in the range of 15-20.degree. C. to obtain solid D(-)-phenylglycine product; andisolating the product obtained in step b) by adjusting the pH.