Patent ID: 6391652
Filing Date: 2002-05-21
Classification: G01N,Y10S

Abstract:
An immunoassay method for detecting bacterial infection by Verotoxin-producing Escherichia coli, in a test sample comprising:(a) bringing an immobilized phase comprising, at different positions on a water-absorbable base material, at least two first immunity substances that specifically bind to at least two different kinds of assay target substances wherein one of the assay target substances is human hemoglobin and the other assay target substances are selected from the group consisting of verotoxin-producing Escherichia Coli and verotoxin, into contact with (1) the test sample; and (2) a liquid containing second immunity substances, each of said second immunity substance is labeled with colored latex particles and binds with said assay target substances, thereby to form assay target substance-labeled immunity substance complexes and to bind said complexes with respective first immunity substances at the immobilized phase; and (b) detecting said labeled immunity substance complex on said water-absorbable base material and relating the presence of said complexes to the presence of said analytes in the test sample.