Patent ID: 8585883
Filing Date: 2013-11-19
Classification: G01N

Abstract:
1. A method for preconcentrating and isolating a plurality n of charged analytes (A i , with i=1 to n) contained in a sample by isotachophoresis, each one of said analytes A i having an effective electrophoretic mobility μ Ai , said effective electrophoretic mobilities μ Ai obeying the fully ordered relationship μ A1 >μ A2 >etc.>μ An , by means of an apparatus comprising: a main separation channel (C) with a proximal end (P) and a distal end (D), means for loading a supplemented sample portion into an internal segment of said main separation channel located between said proximal end and said distal end, means for applying an electric field (Ê) between said proximal end (P) and said distal end (D), a plurality n of extraction channels (E means for detecting focused zones within said separation channel (C) and/or means for measuring a flow velocity along a longitudinal axis of said separation channel under the influence of an electric field (Ê) applied along said axis by said means for applying an electric field, means for applying a transversal flow along each one of said extraction channels (E said method comprising the steps of: