Patent ID: 9073988
Filing Date: 2015-07-07
Classification: C07K,C12N

Abstract:
1. A fed batch method for making an anti-TNFα antibody comprising a light chain variable region (LCVR) comprising the sequence of SEQ ID NO:1 and a heavy chain variable region (HCVR) comprising the sequence of SEQ ID NO:2, said method comprising culturing mammalian cells comprising a nucleic acid encoding said anti-TNFα antibody in a cell culture production medium in large scale, wherein the pH of the cell culture production medium is adjusted according to a pH linear ramp comprising beginning at a starting pH and ending at a final pH that is less than the starting pH, such that said anti-TNFα antibody is produced, and wherein said produced anti-TNFα antibody is further affinity purified using a Protein A resin.