Patent ID: 8278056
Filing Date: 2012-10-02
Classification: C07K,C12Q,G01N

Abstract:
1. A method of detecting a papillomavirus protein in a human subject, comprising: preparing a cell lysate solution using a sample of cells from the human subject, wherein the sample of cells comprises epithelial cells; coating a portion of a solid surface with a first coat of the cell lysate solution; reacting the cell lysate solution with an anti-HPV monoclonal antibody, wherein the monoclonal antibody specifically binds to a native HPV E7 protein or a native HPV E6 protein from more than one papillomavirus type, and is capable of binding in situ to the protein in a clinical sample; forming a complex of the papillomavirus proteins HPV E6 or E7 with the antibody on the portion of the solid surface; detecting the complex on the portion of the solid surface, wherein presence of the complex indicates presence of the papillomavirus protein present in the human subject; and determining disease stage associated with papillomavirus infection in the human subject based at least in part on the presence of the complex, wherein the presence of the complex indicates the disease stage as a late dysplasia stage.