Patent ID: 8709757
Filing Date: 2014-04-29
Classification: C07K,C12N

Abstract:
1. A process for producing an interferon alpha 5 (IFNa5) protein by expression in an Escherichia coli host cell which comprises: a) providing an IFNa5 producing b) culturing the IFNa5 producing c) isolating, and optionally purifying, the expressed IFNa5 protein, wherein said addition of carbon feed solution results in a concentration from about 28.5 to about 36.9 mg iron (III) chloride hexahydrate, from about 3.8475 to about 4.9815 mg calcium chloride dihydrate, from about 6.4125 to about 8.3025 mg zinc (II) sulfate heptahydrate, from about 1.425 to about 1.845 mg manganese (II) sulfate monohydrate, from about 2.85 to about 3.69 mg copper (II) sulfate pentahydrate, from about 1.083 to about 1.4022 mg cobalt (II) chloride hexahydrate, from about 0.285 to about 0.369 mg sodium molybdate dihydrate and from about 0.6555 to about 0.8487 mg boric acid per liter of said fermentation medium.