Patent ID: 7405070
Filing Date: 2008-07-29
Classification: C07D,C12P

Abstract:
1. A method for preparing (S)-indoline-2-carboxylic acid methyl ester by use of a hydrolytic enzyme, comprising the following steps: reacting racemic indoline-2-carboxylic acid with methanol and thionyl chloride, to give racemic indoline-2-carboxylic acid methyl ester; selectively hydrolyzing (R)-form of the racemic indoline-2-carboxylic acid methyl ester in a buffer solution by use of the hydrolytic enzyme to produce (S)-indoline-2-carboxylic acid methyl ester; and separating and recovering the (S)-indoline-2-carboxylic acid methyl ester, wherein said hydrolytic enzyme is selected from the group consisting of Savinase, Alcalase, Novozym 243, Everlase, Esperase, Protease 7, and Acylase.