Patent ID: 6180135
Filing Date: 2001-01-30
Classification: B82Y,G01N,Y02A,Y10S

Abstract:
A method of making polymerized liposomes capable of changing color in the presence of an analyte, comprising:a) providing: i) a plurality of diacetylene lipid monomers; ii) one or more ligands selected from the group consisting of proteins, antibodies, peptides, carbohydrates, nucleic acids, and combinations thereof; iii) one or more organic solvents; and iv) an aqueous solution;b) combining said lipid monomers, said one or more ligands, and said one or more organic solvents to produce a solvent mixture;c) evaporating said one or more organic solvents in said solvent mixture to produce a concentrated lipid-ligand mixture;d) adding said aqueous solution to said concentrated lipid-ligand mixture to produce an aqueous lipid-ligand mixture;e) agitating said aqueous lipid-ligand mixture under conditions while maintaining a temperature above the phase transition temperature of said lipid monomers to produce an agitated lipid-ligand mixture;f) cooling said agitated lipid-ligand mixture to at least about 4.degree. C. to produce liposomes; andg) polymerizing said liposomes to produce polymerized liposomes capable of changing color upon the binding of one or more analytes to said one or more ligands.