Patent ID: 8426143
Filing Date: 2013-04-23
Classification: C07H,C07K,G01N,Y10T

Abstract:
1. An immunoassay for detecting gemcitabine in a sample comprising providing a mixture of a) said sample, b) an antibody selectively reactive with gemcitabine and not substantially cross-reactive with 2′,2′-difluoro-2′-deoxyuridine and tetrahydrouridine, and c) a conjugate of a carrier having either a reactive thiol or amino group with a compound of the formula: wherein B is Y is an organic spacing group; X is a functional group capable of binding to said carrier through said amino or thiol group; and p is an integer from 0 to 1 or salts thereof; causing the gemcitibine in the sample and said conjugate in said mixture to bind with said antibody and thereafter measuring the amount of said conjugate in said mixture which is bound or unbound to said antibody whereby the presence of gemcitibine in the sample can be determined.