Patent ID: 8148152
Filing Date: 2012-04-03
Classification: A61P,C12N

Abstract:
1. A method for producing embryoid bodies (EBs) from pluripotent cells comprising (i) obtaining a liquid single cell suspension culture of pluripotent cells; (ii) collecting and suspending the cells in a container to a density of about 0.5×10 (iii) rocking the container containing the liquid single cell suspension culture thereby generating cell aggregates; and (iv) diluting the suspension, and further rocking a container containing the suspension until formation of EBs; wherein the final concentration of EBs in the suspension culture is about 500 EBs/ml; wherein the pluripotent cells are selected from the group consisting of embryonic stern (ES) cells, primordial germ (EG) cells and non-embryonic pluripotent stem cells.