Patent ID: 7300753
Filing Date: 2007-11-27
Classification: C07K,C12Q,G01N

Abstract:
1. A method for isolating a target population of naturally-occurring post-translatiorially modified peptides from a complex mixture of peptides, said method comprising the steps of: (a) obtaining a digested proteinaccous preparation from an organism, wherein said digested proteinaccous preparation comprises a complex mixture of peptides comprising naturally-occurring post-translationally modified peptides from two or more different proteins and further fractionating said post-translationally modified peptides in said digested proteinaceous preparation by reversed-phased chromatography to produce a fractionated proteinaceous preparation; (b) contacting said fractionated proteinaccous preparation with at least one immobilized post-translational modification-specific antibody; and (c) isolating said target of naturally-occurring post-translationally modified peptides specifically bound by said immobilized inodification-specific antibody in step (b).