Patent ID: 6683171
Filing Date: 2004-01-27
Classification: A61K,A61P,C07K,G01N

Abstract:
A method of detecting the presence of a Fkhsf nucleic acid molecule in a biological sample, comprising the steps of:(a) contacting a Fkhsf specific nucleic acid probe under high stringency hybridizing conditions with at least one nucleic acid molecule from the biological sample, wherein the probe comprises a sequence that corresponds to a region within a domain sequence of a Fkhsf nucleic acid molecule, said Fkhsf nucleic acid molecule comprising the sequence set forth in SEQ ID NO:1, wherein the domain sequence encodes a domain of a Fkhsf polypeptide, said domain selected from the group consisting of (i) an Amino Terminal domain comprising the sequence set forth at amino acid positions 1-197 of SEQ ID NO:2; (ii) a Zinc Finger domain comprising the sequence set forth at amino acid positions 198-221 of SEQ ID NO:2; (iii) a Middle domain comprising the sequence set forth at amino acid positions 222-336 of SEQ ID NO:2; and (iv) a Forkhead domain comprising the sequence set forth at amino acid positions 337-429 of SEQ ID NO:2; and (b) detecting the formation of a hybrid of said nucleic acid probe and the nucleic acid molecule from the biological sample, and therefrom detecting the presence of a Fkhsf nucleic acid molecule in the biological sample.