Patent ID: 9046516
Filing Date: 2015-06-02
Classification: G01N

Abstract:
1. An immunoassay method comprising: forming, on a solid phase, a complex comprising a measurement substance in a sample, an alkaline phosphatase (ALP) labeling substance capable of binding to the measurement substance, and a first binding substance which is capable of binding to the measurement substance by antigen-antibody reaction and is capable of binding to the solid phase; contacting the complex bound to the solid phase, after said forming step, with an auxiliary reagent solution having a pH value of 4-7 comprising an inhibitor for inhibiting an endogenous ALP, wherein the inhibitor is selected from the group consisting of levamisole, homologues of levamisole whose phenyl ring is substituted with a C1 to C6 lower alkyl group, homologues of levamisole whose phenyl ring is substituted with a halogen atom, and tetramisole; reacting ALP in the ALP-labeling substance bound to the measurement substance in the complex, after said contacting step, with a substrate for ALP contained in a substrate solution having a pH value of 9-11, in the presence of the auxiliary reagent solution to form a mixture; and detecting, after said reacting step, a reaction product produced by the reaction between ALP and the substrate, wherein each of the substrate solution and the auxiliary reagent solution comprise a buffer agent, and the substrate solution has a higher buffering ability than that of the auxiliary reagent solution, and wherein the substrate solution is accommodated in a first reagent container, and the auxiliary reagent solution is accommodated in a second reagent container different from the first reagent container.