Patent ID: 6461837
Filing Date: 2002-10-08
Classification: C07K,C12N

Abstract:
A method for producing a polypeptide, comprising:(a) cultivating a fungal host cell in a medium conducive for the production of the polypeptide, wherein the fungal host cell comprises a first nucleic acid sequence encoding the polypeptide operably linked to a second nucleic acid sequence comprising a consensus translational initiator sequence foreign to the first nucleic acid sequence wherein the 3â€² end of the consensus translational initiator sequence is immediately upstream of the initiator codon of the first nucleic acid sequence, and the consensus translational initiator sequence comprises the sequence 5â€²-NYCNNHCACC-3â€² (SEQ ID NO:1) wherein N is a nucleotide selected from the group consisting of adenine (A), guanine (G), cytosine (C), and thymine (T); Y is a cytosine (C) or thymine (T); and H is a nucleotide selected from the group consisting of adenine (A), cytosine (C), and thymine (T); and (b) isolating the polypeptide from the cultivation medium.