Patent ID: 8034617
Filing Date: 2011-10-11
Classification: A61P,C12N

Abstract:
1. A method for culturing diploid anchorage-dependent cells, the method comprising: a) seeding diploid anchorage-dependent cells onto a first cell-supporting substrate in a cell culture medium, wherein the cell culture medium is devoid of exogenous components of primary and secondary animal origin, and which comprises: i) at least one exogenous growth factor of non-animal origin selected from the group consisting of EGF, FGF, tri-iodo-L tyronine and hydrocortisone, ii) at least one exogenous growth factor of non-animal origin selected from the group consisting of IGF-1 and insulin, and iii) a wheat protein hydrolysate; b) letting the cells adhere to the substrate and multiply; c) harvesting the culture medium; d) detaching the cells from the substrate and dissociating the cells with a protease of non-animal origin, thereby forming a cell suspension; e) inoculating a portion of the cell suspension onto a second cell-supporting substrate in a cell culture medium as defined in step a).