Patent ID: 8685667
Filing Date: 2014-04-01
Classification: C07K,C12N,C12Q,G01N

Abstract:
1. An isolated nucleic acid molecule comprising a polynucleotide sequence encoding beta strands 1-10 of a fluorescent protein comprising a polypeptide sequence at least 95% identical to: the sequence set forth as SEQ ID NO: 4, wherein residue 39 is I, residue 105 is K, residue 111 is V, residue 128 is T, residue 166 is T, residue 167 is V and residue 205 is T; or the sequence set forth as SEQ ID NO: 6, wherein residue 80 is Q, residue 99 is Y, residue 105 is N, residue 111 is V, residue 128 is T, residue 166 is T, residue 172 is V and residue 205 is T; and wherein the beta strands 1-10 of the fluorescent protein are capable of self-complementation with a polypeptide corresponding to a beta strand 11 of the fluorescent protein.