Patent ID: 6087502
Filing Date: 2000-07-11
Classification: C07D,C09K,G01N

Abstract:
A chemiluminescent compound represented by formula (I): ##STR19## wherein A is a linker selected from a C.sub.1-4 alkylene group which may optionally have at least one substituent; Z is a binding group selected from the group consisting of a carboxyl group, a cyano group, an isocyanate group, an isothiocyanate group, an azide group, a sulfonyl group, a halosulfonyl group, a halogenated carbonyl group, an N-succinimidyloxycarbonyl group, an N-phthalimidyloxycarbonyl group, and a maleimidyl group; Y is a counter ion; R.sup.1 and R.sup.2 are independently a methyl, ethyl, methoxy, ethoxy, chlorine, bromine or fluorine; p and m are independently an integer of from 0 to 4; n is an integer of from 2 to 5; and X is an organic intervening moiety with a valence of n, having a phenyloxy group and X binds to a carbonyl carbon bonded to the 9-position of the acridinium ring through the oxygen of the phenyloxy group, wherein said phenyloxy group is substituted at its ortho position with an alkyl group, an aryl group or an alkoxy group and if a plurality of phenyloxy groups is present, the plurality of phenyloxy groups are mutually bound with a directly bound alkyl group, carbonyl group, sulfonyl group, sulfinyl group or aryl group; andwhich exhibits i) a luminescence of at least 2.4.times.10.sup.20 counts/mol, andii) residual luminescence activity of at least 50% of the original luminescence when the compound is stored in a buffer of pH 8.0 at 45.degree. C. for one week in the dark.