Patent ID: 7060447
Filing Date: 2006-06-13
Classification: A61P,G01N,Y10T

Abstract:
1. A method to identify an antidote for a toxic compound which method comprises: observing an intracellular localization pattern of at least one signal transduction protein in the presence and absence of the toxic compound, said compound supplied at a concentration that is toxic so as to ascertain the intracellular localization pattern under toxic conditions, defined as in the presence of toxic compound, and under normal conditions, defines as in the absence of toxic compound; observing the intracellular localization pattern of said at least one signal transduction protein under toxic conditions in the presence of compound that is a candidate for an antidote; comparing the intracellular localization pattern under toxic conditions in the presence of the compound that is a candidate for an antidote to the intracellular localization patterns under toxic and normal conditions; whereby said compound that is a candidate for an antidote whose presence under toxic conditions restores the intracellular localization pattern to an intracellular localization pattern more closely resembling that under normal conditions is identified as an antidote to the toxic compound; wherein said intracellular localization pattern is constructed by determining the presence, absence or level of said signal transduction protein(s) at at least three of nuclear, perinuclear, diffuse cytoplasmic, cytoplasmic fibril-associated and membrane-associated locations.