Patent ID: 7714111
Filing Date: 2010-05-11
Classification: C07K

Abstract:
1. A method for isolating a product, the method comprising: (a) providing a load fluid comprising a product and one or more impurities, wherein the product is an Fc-containing monoclonal antibody; (b) contacting the load fluid with a medium, wherein the medium is a Protein A chromatography column, and wherein the medium can bind the product under conditions suitable for binding the product, thereby providing a bound medium; (c) contacting the bound medium with one or more wash solutions, wherein at least one wash solution comprises an arginine derivative, thereby providing a washed medium, wherein the arginine derivative is acetyl arginine, agmatine, arginic acid, N-alpha-butyroyl-L-arginine or N-alpha-pyvaloyl arginine; (d) contacting the washed medium with an elution solution under conditions suitable for eluting the product, and (e) collecting an eluate comprising the product.