Patent ID: 6054274
Filing Date: 2000-04-25
Classification: C12Q

Abstract:
A method of amplifying the signal of target nucleic acid sequence analyte, comprising the steps of:(1) hybridizing a first end of a bidirectional primer to a target nucleic acid sequence;(2) polymerizing at said hybridized first end of said bidirectional primer to form a complementary nucleic acid sequence of said target nucleic acid sequence;(3) hybridizing a second end of said bidirectional primer to a circular DNA template; and(4) polymerizing at said hybridized second end of said bidirectional primer using said circular DNA template in the presence of a multiplicity of signal amplification sequence units to form a complementary nucleic acid sequence of said target nucleic acid sequence to which are covalently attached said repeating signal amplification sequence units,wherein said bidirectional primer contains a first end complementary to said target nucleic acid sequence and a second end complementary to said circular DNA template.