Patent ID: 7276338
Filing Date: 2007-10-02
Classification: B82Y,C12Q,G16B,Y10T

Abstract:
1. A method of obtaining sequence information at N nucleobase positions of a single copy of a target oligonucleotide, the method comprising the steps of: a) exposing the target oligonucleotide to one or more copies of a test oligonucleotide of M potentially informative nucleobases, wherein b) measuring the hybridization of the test oligonucleotide to the target oligonucleotide, thereby completing a first hybridization trial; c) dehybridizing the test oligonucleotide; d) exposing the target oligonucleotide to one or more copies of at least one additional test oligonucleotide of M potentially informative nucleobases; e) measuring the hybridization of the additional test oligonucleotide to the target oligonucleotide, thereby completing a further hybridization trial; and f) repeating steps c) to e) until the information content from the hybridization trials exceeds the information content of the target oligonucleotide, wherein the information content from a single hybridization trial is where P(M,N) is the probability that the M potentially informative nucleobases fully hybridize with a given target oligonucleotide of N nucleobases.