Patent ID: 6103465
Filing Date: 2000-08-15
Classification: C12Q

Abstract:
A method useful for typing HLA class I genes comprising the steps of:providing a sample DNA containing a HLA class I gene having a first exon, a second exon, and a target sequence;wherein the HLA class I gene is selected from the group consisting of HLA-A gene, HLA-B gene, and HLA-C gene;wherein the first exon is exon 1 of the HLA class I gene and the second exon is exon 5 of the HLA class I gene;contacting the sample DNA with a first amplification primer, the first amplification primer including a sequence complementary to the first exon of the HLA class I gene;contacting the sample DNA with a second amplification primer, the second amplification primer including sequence complementary to the second exon of the HLA class I gene;amplifying the target sequence of the sample DNA by PCR using the first and second amplification primers, thereby forming an amplicon; anddetecting the amplicon using a sequence-specific detection means.