Patent ID: 6329138
Filing Date: 2001-12-11
Classification: C12Q

Abstract:
A method for the detection of the resistance to rifampicin and rifampicin analogues by M. tuberculosis present in a sample, comprising:(i) providing polynucleic acids from said sample;(ii) hybridizing under appropriate hybridization and wash conditions the polynucleic acids of step (i) with a set of probes comprising at least five rpoB gene S-probes, wherein said probes taken together cover the mutation region of the rpoB gene of M. tuberculosis, said S-probes being selected from the group consisting of:S1 (SEQ ID NO:1), S11 (SEQ ID NO:2), S2 (SEQ ID NO:3), S3 (SEQ ID NO:4), S33 (SEQ ID NO:5), S4 (SEQ ID NO:6), S44 (SEQ ID NO:7), S444 (SEQ ID NO:43), S4444 (SEQ ID NO:8), S5 (SEQ ID NO:9), S55 (SEQ ID NO:10), S555 (SEQ ID NO:39), S5555 (SEQ ID NO:40), S55C (SEQ ID NO:44), S55M (SEQ ID NO:45), S6 (SEQ ID NO:11), and S66 (SEQ ID NO:12);(iii) detecting the hybrids formed in step (ii); and(iv) inferring resistance to rifampicin and rifampicin analogues by said M. tuberculosis present in said sample by the absence of hybridization with at least one of said S-probes.