Patent ID: 6458554
Filing Date: 2002-10-01
Classification: A61L,C12Q,G01N

Abstract:
A method for determining the effectiveness of a disinfection or sterilization process, said method comprising;providing a carrier with microorganisms on the carrier; exposing said carrier to the disinfection or sterilization process; incubating said carrier in a growth medium comprising a first buffer having a first pKa, a second buffer having a second pKa, and a pH-sensitive dye, wherein said incubating is after said exposing; and determining whether said microorganisms grow in said growth medium during said incubating, wherein the growing of said microorganisms in said growth medium generates acid, thereby changing pH in said growth medium from a first pH within a first pH range to a second pH within a second pH range, wherein said first pKa and said second pKa are within said first pH range and said second pH range respectively.