Patent ID: 7951576
Filing Date: 2011-05-31
Classification: C12N

Abstract:
1. A method for preparing cells competent for poxvirus replication from a biological tissue, the method comprising: (a) providing a biological tissue comprising avian primary cells susceptible to infection with a poxvirus that replicates in the cells; (b) exposing the biological tissue to a solution comprising a protease, and agitating the biological tissue in an orbital shaker running at a speed of at least 95 revolutions per minute (rpm), thereby causing cells of the biological tissue to be dissociated and be released into the solution; (c) inactivating the protease in the solution; (d) separating the released cells from the non-dissociated tissue components; and (e) infecting the released cells with the poxvirus, wherein the released cells are not centrifuged at any point prior to infection with the poxvirus.