Patent ID: 9176117
Filing Date: 2015-11-03
Classification: C12N,G01N

Abstract:
1. A system for the detection of an energetic material comprising: an isolated stationary macrophage cell isolated from a vestibular labyrinth of a said cell contacting a substrate selected from the group consisting of glass; said cell contacting one or more energetic materials selected from the group consisting of 1,3,5-trinitro-1,3,5-triazacyclohexane, 2,2-bis(hydroxymethyl)1,3-propanediol, 1,3,5,7-tetranitro-1,3,5,7-tetrazocane, 2,4,6-trinitrotoluene, C4, Semtex Al, 1,2,3-trinitroxypropane, and 2,3-dimethyl-2,3,-dinitrobutane; said cell exhibiting a set of cellular responses characteristic of and specific to said energetic material, said cellular responses selected from the group consisting of NOS activity, plateau of NO levels, sustainment of NO levels, increases or decreases in double stranded DNA, increases in nuclear DNA levels, unscheduled DNA synthesis, increase in cell size (diameter on surface), increased membrane surface, membrane blebbing, membrane peroxidation, increased cytoskeleton, enlarged nucleus, lobed nucleus, nucleus with tines, nuclear swell-relax, cytoplasmic DNA, aborization of the nucleus, formation of micronuclei, chromosome condensation, karyomeres, mirror image cells, breakdown of the nuclear envelope, onset of mitosis, emergence from tissue, hunting behavior, induction of apoptosis, and combinations thereof.