Patent ID: 6586586
Filing Date: 2003-07-01
Classification: C07H

Abstract:
A method of purifying an oligonucleotide from a mixture in solution, said mixture including said oligonucleotide and at least one contaminant comprising:treating said mixture with an amino reactive reagent for a time and under conditions effective to form a linkage of formula â€ƒwith each said contaminant; and separating said oligonucleotide from said linked contaminants; wherein the amino reactive reagent is selected from the group consisting of a polymeric support modified by attachment thereto of an amino reactive group Z and a compound of the formula â€ƒwherein: x is from 0-20; y is from 0 to 5; n is from 0 to 150; and Z is â€”NH2, â€”NHâ€”NH2, â€”Oâ€”NH2, â€”NHâ€”C(O)â€”NHâ€”NH2, or NHâ€”C(S)â€”NHâ€”NH2.