Patent ID: 8173784
Filing Date: 2012-05-08
Classification: C07C,C07H,C07K,C12Q,G01N

Abstract:
1. A substrate for analysis of an enzyme having formula: where A is a monosaccharide or a disaccharide and B 1 is a C 1 -C 20 alkyl, a substituted C 6 -C 20 aryl, C 6 -C 20 heterocyclic containing a heteroatom of N, O or S, said B 1 covalently associated with A; B where R 1 ′ is a C 1 -C 20 alkyl, C 1 -C 20 alkyl having a substituent of N, O, or S, heteroatom containing C 6 -C 20 aryl, C 2 -C 20 carbonyl, C 1 -C 20 amidyl, C 1 -C 20 ether, C 6 -C 20 aryl, C 6 - C 20 heterocyclic containing a heteroatom of N, O, or S, said R1′ covalently bound to both B 1 and B 3 ; X is a nullity, oxygen, sulfur, or nitrogen; R 2 ′ is a C 1 -C 20 alkyl, C 4 -C 20 ether, C 1 -C 20 alkyl having a substituent of N, O, or S, C 1 -C 20 ester, C 1 -C 20 alcohol, C 1 -C 20 alkenyl, heteroatom containing C 6 -C 20 aryl, or a C 6 -C 20 heterocyclic containing a heteroatom of N, O or S; Y is a nitrogen, oxygen, or sulfur nucleophilic group; and n is an integer between 1 and 30; B where R 1 is a C 1 -C 20 alkyl, C 1 -C 20 alkyl having a substituent of N, O, or S, a heteroatom containing C 6 -C 20 aryl, C 2 -C 20 carbonyl, C 1 -C 20 amidyl, C 1 -C 20 ether, C 6 -C 20 aryl, or a C 6 -C 20 heterocyclic containing a heteroatom of N, O, or S, said R 1 covalently associated with B 2 , said R 1 covalently associated with said B 4 directly or through an intermediate (XR 3 )n-R 4 ; R 2 is independently in each occurrence a C 1 -C 20 alkyl, a C 2 -C 20 alkyl having a substituent of C 1 -C 20 alkyl; X is independently in each occurrence a nullity, oxygen, sulfur, or nitrogen; R 3 is independently in each occurrence a nullity, C 1 -C 20 alkyl, C 1 -C 20 having a substituted C 6 -C 20 aryl, C 6 -C 20 heterocyclic containing a heteroatom of N, O or S; n is an integer between 0 and 30, inclusive; R 4 is independently in each occurrence a nullity, C 1 -C 20 alkyl, C 1 -C 20 having a substituted C 6- C 20 aryl, C 1 -C 20 carbonyl, C 1 -C 20 amidyl, C 1 -C 20 ether, C 6 -C 20 aryl, C 6 -C 20 heterocyclic containing a heteroatom of N, O, or S; and B