Patent ID: 8299335
Filing Date: 2012-10-30
Classification: C12N

Abstract:
1. A corn seed comprising a vitreous kernel phenotype, at least one genetic element that comprises one or more recombinant DNA(s) encoding one or more transgene(s) that reduce(s) expression of both 19 kilodalton (kD) and 22 kilodalton (kD) alpha-zein storage relative to a control seed, and at least one opaque modifier locus that imparts said vitreous kernel phenotype to said seed comprising said genetic element, wherein a 27 kilodalton gamma zein storage protein content in said corn seed is less than 2-fold higher than that of a seed of the same variety that contains said genetic element but lacks said opaque modifier, and wherein said vitreous seed has a kernel density of at least about 1.24 grams/milliliter, and wherein said opaque modifier comprises at least one genetic locus present in corn line I226211 germplasm deposited as seed under ATCC Accession No. PTA-8214 or wherein said opaque modifier comprises at least one genetic locus present in corn line I283669 germplasm deposited as seed under ATCC Accession No. PTA-8569.