Patent ID: 9051618
Filing Date: 2015-06-09
Classification: A61K,A61P,C12N,C12Q

Abstract:
1. A method of diagnosing whether a subject has, or is at risk for developing, lung cancer, comprising: receiving a test sample of lung tissue extracted from a subject; measuring by Northern blot analysis the level of at least miR-205 gene product and at least one additional miR gene product in the test sample, wherein the Northern blot analysis comprises: extracting nucleic acids from the test sample; treating the extracted nucleic acids with DNase to remove DNA; separating RNA molecules in the extracted nucleic acids by gel electrophoresis; hybridizing the separated RNA molecules to labeled probes; and detecting and quantifying the hybridization to determine the level of at least miR-205 gene product and at least one additional miR gene product; comparing the level of at least miR-205 gene product and at least one additional miR gene product in the test sample to a control level of at least one corresponding miR-205 gene product and at least one additional miR gene product in normal lung tissue; and diagnosing whether the subject has, or is at risk for developing, lung cancer, if the level of the at least miR-205 gene product and at least one additional miR gene product in the test sample from the subject is greater than the control level of at least one miR-205 gene product and at least one corresponding additional miR gene product.