Patent ID: 6716429
Filing Date: 2004-04-06
Classification: A61K,C07K,C12N

Abstract:
An isolated and purified protein encoded by nucleotide sequence encoding a modified HIV-1 or HIV-2 envelope glycoprotein containing at least:i) a coiled coil portion of a gp41 transmembrane glycoprotein, wherein said coiled coil has a heptad repeat wherein each of said seven consecutive amino acid residues are designated a, b, c, d, e, f, and g corresponding to amino acid sequences selected from a group consisting of amino acids 555-561, 562-568, 569-575, 576-582 and 583-589 of SEQ ID NO: 11, wherein at least two amino acids in positions â€œaâ€ , â€œdâ€  and â€œeâ€  have been substituted by cysteine residues, and â€œfâ€  is glycine; and ii) a gp120 glycoprotein or a gp120 derivative, wherein the gp120 derivative contains multiple gp120 constant regions connected by variable regions and/or linker residues that permit potential turns in the polypeptide structure so that the derivative maintains a conformation approximating that of a wild type gp120, wherein at east a portion of one variable region has been deleted.