Patent ID: 7354730
Filing Date: 2008-04-08
Classification: C12Q,G01N

Abstract:
1. A high-throughput system for rapidly determining the proliferative status of primitive hematopoietic cells by luminescence output comprising: a. a target cell population of mononuclear cells comprising primitive hematopoietic cells; b. a medium comprising serum and methyl cellulose; c. a proliferation agent specific for a single subpopulation of primitive hematopoietic cells, the proliferation agent comprising one or more growth factors, one or more cytokines, or combinations thereof; d. a reagent capable of reacting with ATP and generating luminescence in the presence of ATP; and e. a plate; wherein the target cell population, the medium, the proliferation agent, and the reagent capable of reacting with ATP are combined in an order to determine the proliferative status of the subpopulation of primitive hematopoietic cells by detecting the level of luminescence generated from the reagent that reacted with the ATP, and wherein the level of luminescence detected indicates the amount of ATP in the subpopulation of primitive hematopoietic cells, and the amount of ATP in the subpopulation of primitive hematopoietic cells indicates the proliferative status of the subpopulation of primitive hematopoietic cells.