Patent ID: 6569629
Filing Date: 2003-05-27
Classification: C12Q,Y10S

Abstract:
A method of identifying a bovine animal or carcass having a genetic marker for marbled beef, comprising:(a) extracting DNA from cells in a sample obtained from the bovine animal or carcass to provide a DNA sample; (b) amplifying the DNA sample by a polymerase chain reaction (PCR) which employs a forward primer comprising at its 3â€² end a nucleotide sequence identical to at least 10 contiguous nucleotides within SEQ ID NO. 1 and a reverse primer comprising at its 3â€² end a nucleotide sequence fully complementary to at least 10 contiguous nucleotides within SEQ ID NO. 1, wherein said primers are selected to amplify a predetermined region of SEQ ID NO. 1, said region comprising 50 or more contiguous nucleotides within SEQ ID NO. 1, and wherein said primers i) do not contain runs of more than 5 of the same nucleotide base, ii) do not contain internal palindromic sequences, iii) do not hybridize to one another under stringent conditions, and iv) have 40 to 60 percent G+C content, and wherein said amplification provides a PCR product that is from 50 to 613 nucleotides in length; and (c) characterizing the size or the sequence of the PCR products, wherein the production of a PCR product having a size which is the same as the size of the predetermined region of SEQ ID NO. 1 or a sequence which is identical to or fully complementary to the sequence of the predetermined region of SEQ ID NO. 1 indicates that the animal or carcass has a genetic marker of marbling.