Patent ID: 7951550
Filing Date: 2011-05-31
Classification: C07D,C07F,C09B,C12Q,G01N

Abstract:
1. A method to detect or determine the presence or amount of a molecule for a nonluciferase enzyme-mediated reaction in a sample, comprising: a) contacting said sample with a first reaction mixture for a nonluciferase enzyme-mediated reaction comprising a substrate for said nonluciferase enzyme to yield a first mixture, such that the nonluciferase enzyme converts the substrate into a beetle luciferase substrate, wherein the substrate for said nonluciferase enzyme is a compound of formula IIA: wherein Z is OR Z″ is O, S, NH, NHR, or N═N; W R is H, (C R (C R or when Z is NR wherein any alkyl, alkenyl, alkynyl, cycloalkyl, alkoxy, amino, aryl, heteroaryl, or heterocycle group of R or R or M R when Z is OR A or a salt thereof, and wherein the substrate is not dehydroxyluciferin (H Luc), luciferin 6′-ethyl ether (LucEE), luciferin 6′-4-picolinyl ether (Luc4PE), luciferin 6′-3-picolinyl ether (Luc3PE), luciferin 6′-2-picolinyl ether (Luc2PE), luciferin 6′-4-trifluoromethylbenzyl ether (LucTFMBE), luciferin 6′-phenylethyl ether (LucPEE), luciferin 6′-geranyl ether (LucGE), luciferin 6′-prenyl ether (LucPE), luciferol, D-chloroluciferin, D-naphthylluciferin or D-quinolylluciferin; b) contacting at least a portion of the first mixture with a second reaction mixture for a beetle luciferase-mediated reaction comprising beetle luciferase to yield a second mixture, and c) detecting or determining luminescence in the second mixture, thereby detecting or determining the presence or amount of a molecule for the nonluciferase enzyme-mediated reaction in the sample.