Patent ID: 6710031
Filing Date: 2004-03-23
Classification: A61P,C07K

Abstract:
A protein comprising an amino acid sequence with a homology of not less than 30% to the amino acid sequence of SEQ ID NO: 1 and forms a secondary or tertiary structure composed of a first &bgr; strand (&bgr;1), a first &agr; helix (&agr;1), a second &agr; helix (&agr;2), a second &bgr; strand (&bgr;2), a loop, a third &bgr; strand (&bgr;3), a fourth &bgr; strand (&bgr;4) and a fifth &bgr; strand (&bgr;5) in this order from the amino terminus, and wherein at least one amino acid residue in a region from &agr;2 to &bgr;2, a region from &bgr;3 to &bgr;4, or in the regions from &agr;2 to &bgr;2 and from &bgr;3 to &bgr;4 is replaced so that electric charge of the amino acid residue is changed towards positive direction as compared to the unsubstituted amino acid, said protein being the following (a) or (b):(a) a protein, in which the region from &agr;2 to &bgr;2 has the sequence of the amino acid residues 47 to 72 in the amino acid sequence of SEQ ID NO: 1 and the region from &bgr;3 to &bgr;4 has the sequence of the amino acid residues 94 to 111 in the amino acid sequence of SEQ ID NO: 1; (b) the protein according to (a), in which substitution, insertion or deletion of one amino acid residue is included in the region from &agr;2 to &bgr;2 having the sequence of the amino acid residues 47 to 72 in the amino acid sequence of SEQ ID NO: 1, or the region from &bgr;3 to &bgr;4 having the sequence of the amino acid residues 94 to 111 in the amino acid sequence of SEQ ID NO: 1; and wherein said protein has antithrombotic activity.