Patent ID: 6120988
Filing Date: 2000-09-19
Classification: A61K,C07K,C12N,C12Q,G01N,Y10T

Abstract:
A method of identifying a recombinant antigen which is immunoreactive with an antibody that is induced by HEV and that is present in individuals infected with HEV, said method comprising:(a) recombinantly producing a polypeptide encoded by a nucleic acid derived from an HEV genome, wherein said genome contains a region which hybridizes with the sequence SEQ ID NO:1 and remains hybridized under the following wash conditions: 2.times.SSC, 0.1% SDS, room temperature, twice for 30 minutes each, then 2.times.SSC, room temperature, twice for 10 minutes each;(b) immunoreacting said polypeptide with an HEV-positive antiserum, and(c) selecting said polypeptide as a recombinant antigen if said polypeptide immunoreacts with the HEV-positive antiserum.