Patent ID: 7336355
Filing Date: 2008-02-26
Classification: G01N

Abstract:
1. A fluorescence spectroscopic apparatus comprising: an exciting optical unit configured to irradiate the same sample area with a plurality of excitation lights of different wavelength bands; an optical unit configured to repeatedly guide fluorescences emitted by the sample in response to the respective excitation lights, to respective detection units via respective barrier filters; and a calculation unit configured to perform analysis on the basis of a comparison of output signals corresponding to the fluorescences from the detection units, wherein: the exciting optical unit comprises an exciting light continuous irradiation unit configured to continuously maintain an intensity of an excitation light, and an excitation light varying unit configured to intermittently vary the intensity of respective excitation light by setting up a delay time so that other plurality of excitation lights do not irradiate each other simultaneously; respective barrier filters, which intermittent fluorescences occurred by the intermittent excitation lights pass through, are configured to block out fluorescent wavelength bands in which continuous fluorescences occurred by the continuous excitation lights pass through; and the calculation unit performs analysis based on intermittent signals output from the detection units which detected the intermittent fluorescences, and continuous signals output from the detection units which detected the continuous fluorescences.