Patent ID: 8349810
Filing Date: 2013-01-08
Classification: A61P,C07K,C12N

Abstract:
1. A method for treating human immunodeficiency virus (HIV) infection in a subject, the method comprising: (a) inactivating a gene encoding a CCR5 receptor in an isolated CD4+ T-cell or hematopoietic stem cell using first and second fusion proteins, each fusion protein comprising a zinc finger binding domain engineered to bind to a target site in the gene encoding the CCR5 receptor and a cleavage half-domain, wherein in at least one of the first or second fusion proteins, the cleavage half-domain is closer to the N-terminus and the zinc finger binding domain is closer to the C-terminus; and (b) introducing the cell with the inactivated gene encoding the CCR5 receptor into the subject, thereby treating HIV infection in the subject.