Patent ID: 7192768
Filing Date: 2007-03-20
Classification: A61P,C12N

Abstract:
1. A method for in vitro maturation of oocytes, said method comprising the steps of: (a) culturing one or more germinal vesicle (GV) oocytes in a culture medium for a time period sufficient for cytoplasmatic maturation to occur, wherein the culture medium comprises a nuclear maturation inhibiting substance and one or more gonadotropins and/or one or more growth factors; (b) washing the GV oocytes of step (a) to remove the nuclear maturation inhibiting substance; (c) culturing the washed oocytes of step (b) in a culture medium comprising one or more gonadotropins and/or one or more growth factors and/or meiosis activating sterols (MAS) for a time period sufficient for nuclear maturation to occur.