Patent ID: 6214547
Filing Date: 2001-04-10
Classification: C07K,C12N

Abstract:
A DNA construct for use in transforming a yeast cell encoding a heterologous polypeptide and having the structureSP-LP-(PS)-(S)-(PS)-*gene*,whereinSP is a DNA sequence (presequence) encoding a signal peptide,LP is a DNA sequence encoding a synthetic leader peptide (propeptide) wherein N-linked glycosylation is lacking and does not comprise the sequence Gln Pro Val Ile Ser Thr Thr Val Gly Ser Ala Ala Glu Gly Ser Lou Asp Lys Arg (SEQ ID NO:42),PS is a DNA sequence encoding a protease processing site which is optional in both positions,S is a DNA sequence encoding a spacer peptide which is optional, and*gene* is a DNA sequence encoding a polypeptide.