Patent ID: 6589783
Filing Date: 2003-07-08
Classification: C12N

Abstract:
A vector for expressing a protein in three separate host cells, the vector comprisingthree separate promoters, wherein the first promoter is effective to express a downstream protein coding sequence in one type of host cell, the second promoter is effective to express the downstream protein coding sequence in a second different type of host cell, and the third promoter is effective to express the downstream protein coding sequence in a third different type of host cell, where the hosts are any combination of three selected from the group consisting of prokaryotic, insect, vertebrate, plant and yeast cells; a multiple cloning site downstream of the promoters such that a protein coding sequence inserted into the multiple cloning site is transcribed by one of the three promoters in each of the three different types of host cells; and at least two transcriptional terminators located downstream of the multiple cloning site to terminate transcription of the promoters, one transcriptional terminator effective in bacterial cells and the other effective in eukaryotic cells.