Patent ID: 8993244
Filing Date: 2015-03-31
Classification: G01N

Abstract:
1. An assay for identifying a compound suppressing coronin 1 trimerization, comprising: tagging peptides that are either (i) coronin 1 monomers or (ii) peptides comprising a peptide of SEQ ID NO: 5, with either fluorescence resonance energy transfer (FRET) acceptor or donor fluorescent tags resulting in tagged peptides carrying an acceptor tag and tagged peptides carrying a donor tag, or with yellow fluorescent protein (YFP) fragments resulting in tagged peptides carrying one type of YFP fragment and tagged peptides carrying a fragment complementary to said one type of YFP fragment, wherein the tagged peptides are: delivered inside non-leukocyte cells that do not express coronin 1, or leukocyte cells in which coronin 1 is knocked out or knocked down, and incubated in the presence of one candidate compound, wherein fluorescence is analyzed, and if the candidate compound suppresses fluorescence from FRET or YFP complementation, the candidate compound is identified as a compound suppressing coronin 1 trimerization.