Patent ID: 7745193
Filing Date: 2010-06-29
Classification: A61K,C07K,C12N

Abstract:
1. A recombinant nucleotide sequence which codes upon expression a bifunctional hybrid active-site serine β-lactamase protein, wherein the β-lactamase protein is a class A, C or D β-lactamase protein that bears at least one heterologous sequence in a region located between two neighboring alpha helices of the β-lactamase sequence, wherein the region is selected from the group consisting of: a) a region forming a juncture between alpha helix 8 and alpha helix 9 of TEM-1 β-lactamase; and b) a region forming a juncture between the alpha helices of said class A, C or D β-lactamase, said alpha helices corresponding to the alpha helix 8 and alpha helix 9 of the TEM-1 β-lactamase, wherein the hybrid protein has two functions, wherein, in said bifunctional hybrid protein, the first function is associated with the β-lactamase portion and the second function is associated with the at least one heterologous sequence having a biological function which is different from the first function.