Patent ID: 6322781
Filing Date: 2001-11-27
Classification: A01N,C07K,C12N

Abstract:
A method for the production of insecticidal recombinant baculoviruses comprising:(a) constructing a recombinant insect cell having a first chimeric gene comprising a first nucleic acid fragment encoding a first promoter, the first nucleic acid fragment operably linked to a second nucleic acid fragment encoding a regulatory protein which affects gene expression;(b) constructing a recombinant baculovirus expression vector having a second chimeric gene comprising a third nucleic acid fragment encoding a second promoter that is affected by the regulatory protein encoded by the second nucleic acid, the third nucleic acid fragment operably linked to a fourth nucleic acid fragment encoding an insecticidal protein;(c) introducing the recombinant baculovirus expression vector of step (b) into the recombinant insect cell of step (a);(d) maintaining the recombinant insect cell from step (c) in an in vitro cell culture, under conditions that support baculovirus replication wherein expression of the regulatory protein encoded by the second nucleic acid fragment affects expression of the insecticidal protein; and(e) collecting progeny viruses.