Patent ID: 6969611
Filing Date: 2005-11-29
Classification: C07D,C07K,C12N,C12P

Abstract:
1. A method to prepare actinomycete cells containing at least one nucleic acid molecule, wherein said molecule comprises at least one module that encodes a modular polyketide synthase (PKS) functional in catalyzing the synthesis of a polyketide, said module comprising at least one nucleotide sequence which encodes a PKS acyl transferase (AT) activity; at least one nucleotide sequence which encodes a PKS ketoacyl carrier protein synthase (KS) activity; and at least one nucleotide sequence which encodes a PKS acyl carrier protein (ACP) activity; said module operatively linked to a control sequence, whereby a functional modular PKS is produced in said cells, with the proviso that said module or said control sequence is heterologous to host cells, said method comprising introducing said nucleic acid molecule into actinomycete host cells that is natively produce polyketide.