Patent ID: 7968284
Filing Date: 2011-06-28
Classification: A61K,A61P,C07K,Y10S

Abstract:
1. A method for identifying a modulator of integrin activity comprising: (a) providing a modified integrin I-domain polypeptide comprising (i) the amino acid sequence of SEQ ID NO:6 containing at least one disulfide bond, said polypeptide containing amino acid substitutions selected from the group consisting of Q163C/Q309C and D294C/Q311C; or (ii) the amino acid sequence of SEQ ID NO:5 containing at least one disulfide bond, said polypeptide containing amino acid substitutions selected from the group consisting of K287C/K294C, E284C/E301C, L161C/F299C, K160C/F299C, L161C/T300C, and L289C/K294C, such that said modified I-domain polypeptide is stabilized in a desired conformation, (b) contacting the modified integrin I-domain polypeptide with a test compound; and (c) assaying the ability of the test compound to bind to the modified integrin I-domain polypeptide and modulate the integrin activity, to thereby identify a modulator of integrin activity.