Patent ID: 6040177
Filing Date: 2000-03-21
Classification: A61K,C12N

Abstract:
A method of genetically transducing a T cell, comprising:(a) adding initial isolated T lymphocytes to a culture medium in vitro, said T lymphocytes comprising a T cell receptor complex, wherein said T lymphocytes are isolated by use of an affinity reagent that selects antigen-specific T cells or are isolated by limiting dilution culture of antigen-stimulated T lymphocytes;(b) adding to the culture medium a disproportionately large number of non-dividing peripheral blood mononuclear cells (PBMC) as feeder cells such that the resulting population of cells contains a ratio of at least about 40 PBMC feeder cells for each T lymphocyte in the initial population to be expanded;(c) activating said T cell receptor complex;(d) adding a transduction vector to said culture medium;(e) incubating the culture, thereby producing a population of transduced T lymphocytes which become reversibly quiescent upon withdrawal of exogenous cytokine and are antigen-specific and MHC restricted.