Patent ID: 6132996
Filing Date: 2000-10-17
Classification: B01L,G01N

Abstract:
A method of amplifying target nucleic acid, comprising the steps of:introducing a sample containing target nucleic acid, nucleotides, enzyme and primers into a capillary tube mounted on a support; said capillary tube having a sample position control unit, including a syringe body and a plunger movable within the syringe body, disposed in communication therewith;maintaining first and second regions of said tube at respective first and second temperatures;altering the pressure within said tube by moving said plunger in a reciprocating motion within said syringe body, thereby moving the sample within said tube successively between said first and second regions in a reciprocating motion, such that the sample is cycled between at least two temperatures in a manner effective to result in primer-specific amplification of the target.