Patent ID: 6696304
Filing Date: 2004-02-24
Classification: G01N,Y10S,Y10T

Abstract:
A process for determining the relative average amount of at least one protein of interest, which is immobilized on each of a plurality of particulate solid phases, comprising:(a) separately subjecting, under substantially the same labeling conditions, a plurality of particulate solid phases, to each of which is covalently immobilized an unknown average amount of at least one protein of interest, and a plurality of standard particulate solid phases to each of which is covalently immobilized a known average amount of at least one reference protein, to labeling conditions effective to provide protein of interest and reference protein both covalently labeled with at least one light-emitting label; (b) separating unbound label from the covalently labeled protein of interest and covalently labeled reference protein; and (c) comparing the average amount of light emitted by labels found on the standard particulate solid phases harboring the reference protein with that emitted by labels found on particulate solid phases harboring the protein of interest to provide a relative average amount of the protein of interest covalently immobilized on each of the particulate solid phases.