Patent ID: 8440959
Filing Date: 2013-05-14
Classification: A61B,G01J

Abstract:
1. A method for simultaneous calibration and spectral imaging of a sample comprising: simultaneously illuminating the sample and an intrinsic calibrant with a plurality of illuminating photons from a single source; receiving, at a spectrometer, a first plurality of photons collected from the sample and a second plurality of photons collected from the calibrant; forming a calibrant spectrum from the second plurality of collected photons and a sample spectrum from the first plurality of collected photons, wherein said sample spectrum comprises a Raman spectrum; directing the calibrant spectrum to a first processor and the sample spectrum to a second processor; configuring the first processor to compare the calibrant spectrum with a reference spectrum of the calibrant to determine a wavelength-shift in the calibrant spectrum; configuring the second processor to apply the wavelength-shift to the sample spectrum to obtain a calibrated sample spectrum; and forming a spatially-accurate wavelength resolved Raman image of the sample.