Patent ID: 7718409
Filing Date: 2010-05-18
Classification: C12M,C12N,G01N

Abstract:
1. A method of electroporating a cell mass in a controlled manner, the method comprising: (a) applying a voltage across a cell mass; (b) detecting the ratio of electric current through the cell mass to the voltage applied across the cell mass, wherein a change in the ratio indicates a change in the degree of electroporation of the cell mass; (c) determining an irreversible electroporation voltage for the cells of the cell mass, wherein the determining of the irreversible electroporation voltage comprises determining an electroporation current-to-voltage onset point of the cell mass or an electroporation current-to-voltage hysteresis of the cell mass; and (d) adjusting the applied voltage to provide the determined irreversible electroporation voltage of step (c), thereby providing an irreversible electroporation of the cells of the cell mass.