Patent ID: 8586947
Filing Date: 2013-11-19
Classification: C12Q,G01N,G02B

Abstract:
1. A method for detecting nucleic acids, comprising: (a) providing a support structure comprising a first manufactured surface and a second manufactured surface that are separated by a fluid passage, wherein the first manufactured surface and the second manufactured surface are in contact with a fluid in the fluid passage, and wherein an array of nucleic acid sites is distributed on the first manufactured surface and on the second manufactured surface, wherein the nucleic acid sites are present at a density of at least 1,000 sites per square millimeter; (b) detecting the array using an optical system having a numerical aperture of at least 0.6 and at a resolution to distinguish the nucleic acid sites on the first manufactured surface; and (c) detecting the array using the optical system having a numerical aperture of at least 0.6 at a resolution to distinguish the nucleic acid sites on the second manufactured surface.