Patent ID: 6511848
Filing Date: 2003-01-28
Classification: A61K,C12N

Abstract:
A method for the multiplication of lymphocytes in a lymphocyte cell culture, comprising incubating lymphocytes in a suitable cell culture medium which contains, from the beginning of incubation, both a compound selected from group A consisting of IL-2, IL-10, IL-13, IL-14 and IL-15, and, simultaneously, a compound selected from group B consisting of cyclosporine, ascomycin (FK520), tacrolimus (FK506), and aurintricarboxylic acid, wherein said compound of group A and said compound of group B are present in the cell culture medium at a concentration effective for allowing the cells to multiply, and carrying out incubation for at least 10 days, wherein a partial volume of said cell culture medium containing both the group A an the group B compound is renewed during said incubation of at least 10 days.