Patent ID: 8962271
Filing Date: 2015-02-24
Classification: C12Q,G01N

Abstract:
1. A method for assaying a glycated protein in a sample, comprising: contacting the sample with one of: a fructosyl peptidyl oxidase comprising an amino acid sequence having at least 70% identity with the amino acid sequence set forth in SEQ ID NO:1 and a Km value toward fructosyl valyl histidine of 1 mM or less; a fructosyl peptidyl oxidase comprising an amino acid sequence having at least 70% identity with the amino acid sequence set forth in SEQ ID NO:1 and a Vmax/Km value toward fructosyl valyl histidine of 10 U/mg·mM or more; and a fructosyl peptidyl oxidase comprising an amino acid sequence having at least 70% identity with the amino acid sequence set forth in SEQ ID NO:1 and residual activity of 50% or more when heat-treated at 50° C. for 10 minutes; and measuring the amount of the glycated protein oxidized by the fructosyl peptidyl oxidase.