Patent ID: 8992942
Filing Date: 2015-03-31
Classification: A61K,A61P,C07K,G01N,Y10S

Abstract:
1. An in vivo method of diagnosis which discriminates between exposure of a subject to Mycobacterium tuberculosis and vaccination of a subject with the Bacille Calmette Guerin (BCG) strain of Mycobacterium bovis , the method comprising: administering to the skin of the subject a diagnostic reagent containing at least two different antigens having Mycobacterium tuberculosis -specific immunogenic properties, wherein the antigens are isolated polypeptides of the Mycobacterium tuberculosis complex that are not encoded by BCG, said at least two polypeptides including at least one isolated polypeptide selected from the group consisting of (i) a first amino acid sequence comprising the sequence of MTBN4 (SEQ ID NO: 4), (ii) a second amino acid sequence that is an antigenic segment of MTBN4, and (iii) a third amino acid sequence that is identical to said first or second amino acid sequence but has conservative substitutions; and detecting whether or not there is an immune response to the diagnostic reagent at the site of administration.