Patent ID: 6046024
Filing Date: 2000-04-04
Classification: A61L,C07K,C12P,Y10S

Abstract:
In a method for producing a fibrin monomer comprisingsubjecting a fibrinogen-containing composition to a biotinylated enzyme to convert the fibrinogen in said fibrinogen-containing composition to fibrin monomer and form a fibrin monomer/biotinylated enzyme mixture;introducing a material having an affinity for biotin into the fibrin monomer/biotinylated enzyme mixture so that a complex of the affinity material and biotinylated enzyme are formed; andseparating the complex, and thereby said enzyme, from the fibrin monomer;wherein the improvement comprises using, as the material having an affinity for biotin, a stable composition of avidin immobilized on an inert support material, which provides minimal damage to biotin binding activity, comprising:an avidin/inert support composition having at least 1000 biotin binding units of activity per gram of said composition;a bulking agent selected from nonionic water soluble compounds; anda protectant.