Patent ID: 8486663
Filing Date: 2013-07-16
Classification: C07K

Abstract:
1. A method for producing species-specific antibodies comprising: co-culturing B cells and antigen presenting cells (APCs) in the presence of a denatured antigen, generating activated B cells by adding a stimulation media comprising CD40 ligand (CD40L), or an anti-CD40 antibody and one or more of the following cytokine stimuli: a) interleukin-2 (IL-2) and interleukin-21 (IL-21); b) interleukin-5 (IL-5), interleukin-10 (IL-10), and transforming growth factor-beta (TGF-β); c) interleukin-4 (IL-4), interleukin-13 (IL-13), or both IL-4 and IL-13; or d) Interleukin-6 (IL-6); detecting the production of a species-specific antibody; and generating a hybridoma from the activated B-cells, wherein the hybridoma produces the species-specific antibodies.