Patent ID: 7129076
Filing Date: 2006-10-31
Classification: A23G,A61K,A61Q,C11D,C12N,C12Y

Abstract:
1. A Bacillus subtilisin variant comprising a substitution of an amino acid at one or more residue positions of a precursor Bacillus subtilisin wherein said substitution alters the overall charge of the precursor subtilisin resulting in a subtilisin variant that is more positive or less negative relative to the precursor subtilisin, wherein said substitution makes the subtilisin variant more effective than the precursor in a detergent concentration system wherein the detergent components are primarily negatively charged or where the overall charge balance of the detergent components is not positive, and wherein said substitutions are selected from the group consisting of N76D/S103A/V104I/Q109R/Q245R, Q12R/N76D/S103A/V104I/M222S/Q245R, V68A/S103A/V104I/G159D/A232V/Q236H/Q245R/N252K, V68A/N76D/S103A/V104I/G159D/P210R/A232V/Q236H/Q245R, and N62D/S103A/V104I/Q109R/G159D/T213R/M232V/Q236H/Q245R/N248D/N252K, and wherein said positions are numbered according to the positions of the mature subtilisin BPN′ having the amino acid sequence set forth in SEQ ID NO:3.