Patent ID: 8158427
Filing Date: 2012-04-17
Classification: C12M,C12N

Abstract:
1. A method of culturing cells comprising: a. forming a cell culture device that is not compartmentalized by a semi-permeable membrane and comprised at least in part of non porous gas permeable material; b. adding a volume of medium and adding cells into said cell culture device, said cells being an animal cell type; c. placing said cell culture device in a cell culture location that includes ambient gas at a composition for cell culture, the volume of said medium being such that the highest location of said medium resides at a height beyond 2.0 cm from the lowest location of said medium; d. allowing said cells to settle out of said medium by gravity; and e. culturing said cells in a mode of static cell culture after said cells settle out of said medium by gravity and said medium being in contact with said non porous gas permeable material.