Patent ID: 7645612
Filing Date: 2010-01-12
Classification: B01L,G01N,Y10S

Abstract:
1. A method for the simultaneous qualitative and/or quantitative determination of a multitude of analytes comprising: bringing one or more liquid samples to be analyzed for the analytes into contact with measurement areas of a sensor platform, the sensor platform comprising an optical thin film waveguide with a first layer, that is transparent at least at one excitation wavelength, on a second layer that has a lower refractive index than the first layer and is also transparent at least at the excitation wavelength, and at least one grating structure modulated in the first layer for in-coupling of the excitation light into the first layer; at least one array of biological or biochemical or synthetic recognition elements immobilized in measurement areas for detection of different analytes by specific recognition and/or binding of said analytes and/or by specific interaction with said analytes, means for laterally resolved referencing of the excitation light intensity available in the measurement areas and, optionally, means for the calibration of one or more luminescences generated in a near field of the first layer, the measurement areas for detection of different analytes being usable for detection of analytes to be determined and/or as means for calibration purposes; irradiating an excitation light towards the measurement areas; detecting an emission light from the measurement areas; referencing the excitation light intensity available in the measurement areas in a locally resolved way by means of correcting the emission light intensity from measurement areas for detection of different analytes using the means for laterally resolved referencing of the excitation light intensity available in the measurement areas; calibrating one or more luminescences generated in a near-field of the first layer from the measurement areas that result from the binding of one or more analytes to biological or biochemical or synthetic recognition elements immobilized in the measurement areas or from interaction between the analytes and the immobilized recognition elements using the measurement areas for detection of different analytes as means for calibration purposes to establish a calibration curve on each platform; and comparing the emission light intensity from measurement areas for detection of analytes in the sample to be analyzed to the calibration curve for qualitative and/or quantitative determination of the analyte.