Patent ID: 8658351
Filing Date: 2014-02-25
Classification: G01N,Y10T

Abstract:
1. A method of aiding in predicting the effect of an agent on liver function in a subject, comprising: administering a test agent to a subject; measuring levels of all of the following biomarkers: 4-ethylphenylsulfate, 1,5-anhydroglucitol (1,5-AG), 10-nonadecenoate (19:1n9), 2-(4-hydroxyphenyl)propionate, 2′deoxycytidine, 2-methylbutyrylglycine, 3-(4-hydroxyphenyl)lactate, 3-dehydrocholate, 3-hydroxy-2-ethylpropionate,4-hydroxybutyrate (GHB), 4-imidazoleacetate, 4-vinylphenolsulfate, 5-hydroxyhexanoate, alpha-ketoglutarate, anthranilate, beta-hydroxyisovalerate, catechol sulfate, cholate, citrate, dimethylglycine, glycocholate, lactate, malate, mannose, N-acetylaspartate (NAA), p-cresol sulfate, phenol sulfate, phenyllactate (PLA), pyroglutamine, sarcosine (N-methylglycine), succinate, taurine, taurocholate, threonine, trans-4-hydroxyproline, tyramine, 5-oxoproline, gamma-glutamylalanine, gamma-glutamylleucine, gamma-glutamylphenylalanine, gamma-glutamylthreonine, gamma-muricholate, glycochenodeoxycholate, glycodeoxycholate, gulono-1,4-lactone, N4-acetylcytidine, opthalmate, pantothenate, tauro-beta-muricholate, taurochenodeoxycholate, taurocholenate sulfate, taurolithocholate 3-sulfate, 1-docosahexaenoylglycerol (1-monodocosahexaenoin), 2-aminobutyrate, 2′-deoxyinosine, 3-aminoisobutyrate, 7-alpha-hydroxycholesterol, acetylcarnitine, alpha-tocopherol, carnitine, cystathionine, cysteine, cysteinylglycine, diohomo-linoleate (20:2n6), eicosenoate (20:1n9 or 11), gamma-glutamylisoleucine, gamma-glutamylvaline, glucarate (saccharate), glucose-6-phosphate (G6P), glucuronate, glycerate, homoserine (homoserine lactone), hypotaurine, hypoxanthine, isobutyrylcarnitine, N1-methyladenosine, N-acetylserine, pentadecanoate (15:0), propionylcarnitine, taurodeoxycholate, taurolithocholate, xanthine, and xylitol in a biological sample obtained from the subject; and comparing the levels of the biomarkers in the sample to hepatotoxicity-positive and/or hepatotoxicity-negative reference levels of the biomarkers in order to predict the effect of the test agent on liver function in the subject.