Patent ID: 7696406
Filing Date: 2010-04-13
Classification: C12N

Abstract:
1. A method of producing a heterologous polypeptide, the method comprising: a) providing a transgenic plant or a transgenic plant cell comprising a recombinant DNA molecule comprising, in the 5′ to 3′ direction, b) growing the transgenic plant or transgenic plant cell; c) producing an RNA transcript of the DNA sequence in the transgenic plant or the transgenic plant cell, the RNA transcript being a complementary RNA copy of the DNA sequence; d) infecting the transgenic plant or the transgenic plant cell with an RNA nucleic acid encoding an RNA-dependent RNA polymerase operable to recognize the viral RNA replication initiation site and convert the RNA transcript produced by the transgenic plant or the transgenic plant cell to a translatable mRNA, the mRNA having a RNA sequence comprising, in the 5′ to 3′ direction: e) translating the translatable mRNA in the transgenic plant or the transgenic plant cell to form the heterologous polypeptide.