Patent ID: 8765417
Filing Date: 2014-07-01
Classification: B01J,C12Q

Abstract:
1. A method of elongating DNA chains comprising: immobilizing primers for DNA elongation onto a substrate having on the surface thereof a copolymer of a first monomer having a 2-methacryloyloxyethyl-phosphoryl choline (MPC) group, a second monomer having p-nitrophenyloxycarbonyl polyethylene glycol methacrylate (NPMA) group, and a third monomer having a butyl methacrylate (BMA) group, wherein said substrate is obtained by which a base material composed of saturated cyclic polyolefins is coated by or dipped into a liquid containing said copolymer, and then dried, wherein the primer has an amino group, and the primer is immobilized onto the surface of the substrate via a covalent bond formed by the reaction of the amino group of the primer with the p-nitrophenyloxycarbonyl group contained in the NPMA with leaving of the p-nitrophenyloxy group of the p-nitrophenyloxycarbonyl group; introducing a sample containing template DNA fragments or template RNA fragments having desired sequences, an enzyme system for DNA chain elongation and nucleotide monomers into the substrate to thereby form a reaction system containing the substrate and the sample, heating said reaction system up to a temperature causing thermal unfolding of the DNA chains (referred to as “thermal unfolding temperature”, hereinafter); cooling said reaction system down to a temperature for annealing (referred to as “annealing temperature”, hereinafter); and allowing elongation reaction of the DNA chains to proceed in said reaction system.