Patent ID: 9080169
Filing Date: 2015-07-14
Classification: A61K,C07H,C12N

Abstract:
1. A method, comprising the steps of: (a) combining an oligonucleotide, a first DNA polymerase, and a plurality of deoxyribonucleotide triphosphates, thereby forming an extended oligonucleotide comprising an original strand and an extended strand, wherein the extended strand comprises at least one reactive substituent; (b) combining a plurality of modifying compounds and the extended oligonucleotide under reaction conditions, wherein the modifying compound is a compound of formula III: thereby forming a modified extended oligonucleotide comprising the original strand and a modified extended strand; and (c) combining a primer complementary to the second primer binding site, a second DNA polymerase, the modified extended oligonucleotide, and a plurality of deoxyribonucleotide triphosphates thereby creating a duplex with the original strand and displacing the modified extended strand.