Patent ID: 6329199
Filing Date: 2001-12-11
Classification: A61K,C12N

Abstract:
A retroviral vector resistant to inactivation by human serum, said retroviral vector having been produced by a producer cell resistant to lysis by human serum, wherein said producer cell includes:(i) a polynucleotide encoding a retroviral envelope protein, said polynucleotide encoding said envelope protein being obtained from a virus selected from the group consisting of feline endogenous virus RD114, BaEV, SSAV, FeLV-B, NZB virus, avian leukosis virus, and HVJ virus, and(ii) a retroviral vector including a 5' LTR, a 3' LTR, a packaging signal, and at least one polynucleotide encoding a protein or polypeptide of interest, wherein said producer cell does not include the entire viral RNA of feline endogenous virus RD114, BaEV, SSAV, FeLV-B, NZB virus, avian leukosis virus, or HVS virus, and wherein said producer cell is derived from a cell selected from the group consisting of the TE671, HT1080, MV-1-Lu, and human 293 cell lines, and cell lines derived from the TE671, HT1080, Mv-1-Lu, and human 293 lines.