Patent ID: 9169480
Filing Date: 2015-10-27
Classification: C07K,C12N

Abstract:
1. A method for preparing, from one cell, each of a protein, DNA, and RNA, comprising performing nuclear and cytoplasmic separation by: treating the cell with a surfactant solution that lyses cell membranes but does not lyse nuclear membranes; homogenizing the treated cell solution by passing the treated cell solution thus obtained through a first filter which is a channel having a pore size that is impenetrable to cells but allows nuclei to pass therethrough; and further passing the treated cell solution thus obtained through a second filter comprising a membrane having such a pore size as to be impenetrable to nuclei but allow cytoplasmic ribosomes to pass therethrough; and then extracting each of a protein, DNA, and RNA, wherein the second filter is connected to the first filter such that the second filter is placed subsequent to the first filter, and wherein the second filter has a smaller area than the first filter.