Patent ID: 6362000
Filing Date: 2002-03-26
Classification: A61K,C07K,C12N

Abstract:
A method for generating a viral vector particle comprising a viral fusion protein, said method comprising:a) providing to a mammalian cell a DNA sequence encoding said viral fusion protein operably linked to a promoter active in said cell, said fusion protein comprising at least a first and a second amino acid sequence, said first amino acid sequence comprising at least a fragment of a retroviral Vpr amino acid sequence wherein said fragment is capable of being packaged into the viral vector particle, said second amino acid sequence possessing an enzymatic activity; b) providing to said mammalian cell at least one nucleic acid sequence of a retrovirus, said nucleic acid sequence encoding at least a Gag protein, wherein said retroviral nucleic acid sequence is expressed in said cell; and, c) forming from said cell said viral vector particles.