Patent ID: 7552013
Filing Date: 2009-06-23
Classification: B01J,C12Q,C40B

Abstract:
1. A method of making an oligonucleotide array, comprising the steps of: a) hybridizing three or more candidate probes comprising a nucleic acid sequence with a first composition comprising the target nucleic acid sequence; b) determining a first hybridization signal for each candidate probe; c) hybridizing the three or more candidate probes with a second and different composition comprising the target nucleic acid sequence; d) determining a second hybridization signal for each candidate probe; e) calculating a hybridization signal ratio of the first hybridization signal to the second hybridization signal for each candidate probe; f) calculating an average hybridization signal ratio for the three or more candidate probes; g) selecting the candidate probe by comparing the candidate probe's hybridization signal ratio to the average hybridization signal ratio and choosing the candidate probe having a hybridization signal ratio closest to the average hybridization signal ratio as a first probe; and h) constructing an oligonucleotide array comprising a probe comprising the nucleic acid sequence of the first probe.