Patent ID: 8129507
Filing Date: 2012-03-06
Classification: A61K,A61P,C07K

Abstract:
1. A method of purifying a human sweat fraction having basophil activation activity, which comprises: filtering human sweat, removing insoluble matters and collecting the filtrate; mixing the filtrate with a ConA-affinity carrier and collecting the supernatant; fractionating the supernatant by anion exchange chromatography and/or reverse phase column chromatography; and contacting each fraction with basophils and assaying the fractions for basophil histamine releasing activity to obtain a fraction having a histamine-releasing activity, wherein the fraction having a histamine-releasing activity has basophil activation activity, and wherein the fraction having a histamine-releasing activity does not have a basophil histamine-releasing activity after incubation with serum from a subject with atopic dermatitis but retains a basophil histamine-releasing activity after incubation with serum from a subject without atopic dermatitis at 37° C. for 30 minutes.