Patent ID: 7939293
Filing Date: 2011-05-10
Classification: A61K,A61P,C07K

Abstract:
1. A method for the production of C-terminal amidated peptides of the general formula II: wherein AS is a genetically encoded amino acid; n is 5-2000; and X is a basic amino acid or derivatives thereof, m is 1-15; and n and m are whole numbers and wherein i) a host cell transformed with an expression cassette, vector or an in vitro expression system, each containing a nucleic acid sequence encoding formula I: wherein AS is a genetically encodable amino acid; X m Y is at least one neutral charge amino acid; p is 1-10; wherein n is cultured in a nutrient medium, ii) the nucleic acid sequence is expressed, iii) optionally the expressed compounds are released from a precursor peptide by enzymatic or chemical cleavage, iv) the expression products from ii) or the intermediate products from iii) are reacted with an alpha-amidating enzyme after an optional purification step, and v) the C-terminal amidated peptide of the general formula II is purified.