Patent ID: 8642514
Filing Date: 2014-02-04
Classification: C07H,C12N,C12Q,C40B,G01N

Abstract:
1. A method of biopanning ligands specific for target molecule(s), said method comprising: contacting a mixture of a sub-library A of chemical compounds and a sub-library B of chemical compounds with target molecule(s), wherein the sub-library A comprises a first number of chemical compounds each comprising (i) a different chemical moiety (p) than other members of sub-library A and (ii) an oligonucleotide (b), or functional analogue thereof, which oligonucleotide (b) or functional analogue comprises a distinctive sequence tag (b2) coding for the identification of the chemical moiety (p) of its compound and a common self-assembly sequence (b1) capable of performing a combination reaction and of building stable combination reaction product(s) with a common self-assembly sequence (b1′) of at least one chemical compound of the sub-library B, wherein the sub-library B comprises a second number of chemical compounds each comprising (i) a different chemical moiety (q) than other members of sub-library B and (ii) an oligonucleotide (b′), or functional analogue thereof, which oligonucleotide (b′) or functional analogue comprises a distinctive sequence tag (b2′) coding for the identification of the chemical moiety (q) of its compound, and a common self-assembly sequence (b1), wherein complex(es) of said stable combination reaction product(s) and said target molecule(s) form; physically separating the complex(es) from members of sub-library A and sub-library B and their stable combination reaction products which are not bound to target molecule(s); and determining the distinctive sequence tags (b2, b2′), respectively, of the sub-library A and sub-library B members bound to the complex(es), thereby identifying the chemical moiety (p) and the chemical moiety (q) of the stable combination reaction product(s) bound to the complex(es), the chemical moieties (p,q) being peptides or globular proteins.