Patent ID: 6054635
Filing Date: 2000-04-25
Classification: C12N

Abstract:
A chimeric gene, comprising a promoter which in its native form is associated with a nucleotide coding sequence, wherein said nucleotide coding sequence will hybridize to the nucleotide coding sequence of the raspberry E4 gene presented as SEQ ID NO:2, wherein hybridization is conducted at 42.degree. C. in 50% formamide, 5.times.SSC, 5.times.Denhardt's solution, 0.5% SDS and 100 .mu.g/ml denatured carrier DNA, followed by washing two times in 2.times.SSC and 0.5% SDS at room temperature and two additional times in 0.1.times.SSC and 0.5% SDS at 42.degree. C., and said promoter is operably linked to a heterologous nucleotide sequence encoding a product of interest and thereby effects constitutive expression of said product of interest.