Patent ID: 9018005
Filing Date: 2015-04-28
Classification: A61P,C12N,C12Q,G01N

Abstract:
1. A method of deriving a human mesenchymal progenitor cell line from a parental human embryonic stem cell, wherein the mesenchymal progenitor cell line comprises mesenchymal stem cells, the method comprising: (a) providing the parental embryonic stem cell or descendents of the parental embryonic stem cell obtained by dispersing an embryonic stem cell colony with trypsin, wherein the parental embryonic stem cell is a human embryonic stem cell; and (b) culturing the parental embryonic stem cell in the absence of feeder cells in rich media comprising (i) essential nutrients required for cell growth and (ii) serum or serum replacement, wherein the rich media does not comprise additional grown regulators of hormones that promote growth of embryonic stem cells, and wherein the culturing produces mesenchymal progenitor cells which self-renew;