Patent ID: 7105334
Filing Date: 2006-09-12
Classification: A61K,C08B,C12N,C12Q

Abstract:
1. A method of specifically cleaving chondroitin sulfate, comprising: contacting chondroitin sulfate with a modified chondroitinase B, and cleaving the chondroitin sulfate, wherein the modified chondroitinase B has the amino acid sequence of the mature peptide of SEQ ID NO: 2 or conservative substitutions thereof, wherein at least one amino acid residue at a position selected from the group consisting of 116, 184, 213, 219, 245, 250, 271, 272, 296, 298, 318, 333, 363 and 364 of SEQ ID NO: 2 has been substituted or deleted.