Patent ID: 6083746
Filing Date: 2000-07-04
Classification: A61K,A61P,C07K,C12N,C12Y,G01N

Abstract:
An antisense-oriented genetic suppressor element expressing a nucleic acid that establishes or maintains a transformed phenotype in a mammalian cell wherein the genetic suppressor element is identified by a method of isolating genetic suppressor elements, wherein the method comprises the steps of:(a) synthesizing randomly fragmented cDNA prepared from the total mRNA of a cell to yield DNA fragments;(b) transferring the DNA fragments to an expression vector to yield a genetic suppressor element library, wherein the expression vector expresses the DNA fragments in a living cell;(c) genetically modifying the living cells by introducing the genetic suppressor element library into the cells;(d) isolating or enriching for genetically modified living cells containing genetic suppressor elements conferring the transformed phenotype on the cells by selecting the cells under conditions wherein the transformed cells are identifiable;(e) obtaining the genetic suppressor element conferring the transformed phenotype from the surviving genetically modified cells.