Patent ID: 9121009
Filing Date: 2015-09-01
Classification: A61K,A61P,C12N

Abstract:
1. A method of bulking the lower esophageal sphincter (LES) for treating low LES pressure in a mammalian subject in need thereof comprising: (a) isolating skeletal muscle cells explanted from the mammalian subject; (b) cooling the isolated cells from step (a) to a temperature lower than 10° C. and storing the cells for 1-7 days; (c) in a first cell culture container, suspending the skeletal muscle cells from step (b) in a cell culture medium for a period of time between 30 and 120 minutes, thereby adhering at least a portion of the suspended cells to the walls of the first cell culture container; (d) decanting into a second cell culture container the cell culture medium containing the portion of suspended cells that did not adhere to the first cell culture container; (e) incubating the decanted cells for a period of time and under conditions sufficient to attach at least a portion of the cells therein to the walls of the second cell culture container; (f) isolating the adherent cells from the walls of the second cell culture container, thereby obtaining skeletal muscle derived progenitor cells (MDCs); (g) culturing the MDCs obtained from step (f), thereby expanding the number of MDCs; (h) freezing the cultured MDCs from step (g) to a temperature below −30° C.; (i) thawing the frozen MDCs; and (j) administering an effective amount of the thawed MDCs to the LES of the mammalian subject, thereby bulking the LES and treating low LES pressure in the mammalian subject in need thereof.