Patent ID: 6828140
Filing Date: 2004-12-07
Classification: C12N

Abstract:
A recombinant adjustable threshold genetic switch comprising:(a) a first nucleic acid construct comprising an inducible promoter operably associated with a first gene encoding a first repressor protein; and (b) a second nucleic acid construct comprising a promote operably associated with a second gene encoding a second repressor protein, wherein transcription from the promoter is active in the absence of a repressor, and wherein the first repressor protein, when produced, is capable of repressing transcription from the promoter from which transcription is active in the absence of a repressor, and wherein transcription of the first gene encoding the first repressor protein is inducible by an activating agent, and wherein the second repressor protein, when produced, is capable of repressing transcription from the inducible promoter, and wherein the activating agent induces transcription from the inducible promoter by a mechanism other than inhibiting expression or activity of the second repressor and is required for activity of the inducible promoter.