Patent ID: 6274560
Filing Date: 2001-08-14
Classification: C07H,C12N,C12P

Abstract:
A method to produce a desired polyketide which method comprises:(a) providing a functional modular polyketide synthase (PKS) comprising at least a first and second module wherein said PKS has been modified to prevent its utilization of a native starter unit for said modular PKS by inactivation of the catalytic domain of the ketosynthase of said first module, but wherein said PKS is able to incorporate a diketide substrate into at least a triketide, andwhich PKS is a complete erythromycin PKS gene cluster, i.e., 6-deoxyerythronolide B (6-dEB) synthase;(b) adding to said modified PKS a diketide that is a substrate for the modified PKS;(c) incubating the modified PKS and said diketide under conditions wherein said polyketide is synthesized; and(d) optionally recovering the polyketide.