Patent ID: 8071383
Filing Date: 2011-12-06
Classification: C12N

Abstract:
1. A method for generating a transgenic soybean plant comprising the steps of: a) introducing into a soybean cell or tissue a DNA construct comprising at least one first expression construct comprising a promoter active in said soybean plant and operably linked thereto a nucleic acid sequence encoding an enzyme capable of metabolizing D-serine, and b) incubating said soybean cell or tissue of step a) on a selection medium comprising D-serine and/or a derivative thereof in a total concentration from about 0.5 mM to about 100 mM for a time period of at least 5 days, and c) transferring said soybean cell or tissue of step b) to a regeneration medium and regenerating and selecting soybean plants comprising said DNA construct, wherein the promoter active in the soybean plant is selected from the group consisting of: i) a nucleotide sequence comprising the sequence as described by SEQ ID NO: 7 or 8, ii) a nucleotide sequence comprising at least one fragment of at least 50 consecutive base pairs of the sequence as described by SEQ ID NO: 7 or 8, and having promoter activity in soybean, iii) a nucleotide sequence comprising a sequence having at least 60% identity to the sequence as described by SEQ ID NO: 7 or 8, and having promoter activity in soybean, and iv) a nucleotide sequence comprising a sequence capable of hybridizing under conditions equivalent or equal to hybridization in 30% formamide, 1 M NaCl, 1% SDS at 37° C., and a wash in 2×SSC at 50° C. to the sequence as described by SEQ ID NO: 7 or 8, and having promoter activity in soybean.