Patent ID: 7257365
Filing Date: 2007-08-14
Classification: G01N

Abstract:
1. A method for detecting the presence of Hepatitis B Virus (HBV) infection in a patient being tested for HBV infection, comprising: obtaining a serum sample from the patient; subjecting the sample to 2D protein gel electrophoresis to separate proteins contained therein on a 2D electrophoresis gel; staining proteins separated on the electrophoresis gel; scanning the image of stained proteins into an image analysis scanner to obtain an image of serum protein expression profile; and comparing the serum protein expression profile consisting of apolipoprotein A-I (apoA-I), apolipoprotein A-I fragments, haptoglobin β chain, haptoglobin cleaved β-chain, haptoglobin 2 chain, apolipoprotein A-IV (apoA-IV), transthyretin, α1-anitrypsin, α1-antitrypsin fragments/isoforms, and DNA topoisomerases II (topo-II) obtained from said patient to the protein expression profile obtained from HBV-negative and HBV-infected patients, wherein an altered said protein expression profile compared to HBV-negative patient profiles indicates the presence of HBV infection in the patient.