Patent ID: 9096852
Filing Date: 2015-08-04
Classification: A61P,C12N,C12Q,G01N

Abstract:
1. An in vitro method for screening for candidate compounds for attenuating ageing of the skin, and/or for hydrating the skin, comprising the following steps: a. bringing at least one test compound in contact with a sample of isolated pre-senescent neonatal human primary epidermal keratinocytes cultured in vitro, wherein the keratinocytes are obtained after culturing in Medium 154 with added Human Keratinocyte Growth Supplement (HKGS) and continually keeping the keratinocytes in a subconfluent state for at least 37 population doublings; b. measuring the expression level of at least one microRNA in said keratinocytes, wherein the expression level of said at least one microRNA is measured by qRT-PCR or real-time PCR; c. comparing the expression level of said at least one microRNA measured in step b to the expression level of said at least one microRNA in untreated keratinocytes which have not been in contact with said at least one test compound; and d. selecting a test compound in which the expression of said at least one microRNA is inhibited by at least 20% compared with said untreated keratinocytes, wherein the at least one microRNA is selected from the group consisting of miR-138, miR-181a and miR-191.