Patent ID: 7479164
Filing Date: 2009-01-20
Classification: A01N,A61L

Abstract:
1. A process of fixing animal tissue to render it suitable for implantation in living mammals, which process comprises treating said animal tissue with an effective amount of a coupling agent which promotes the formation of amide bonds between reactive carboxyl moieties and reactive amino moieties, in combination with a coupling enhancer, and with a cross-linking agent containing at least two reactive amine moieties, said cross-linking agent containing at least two reactive amine moieties and being present in an amount of at least about 80 millimolar, and said treatment being carried out in a manner which results in the formation of amidated links between said cross-linking agent and reactive moieties carried by the molecules of said animal tissue, whereby said tissue is rendered resistant to protease digestion while incurring only minimal surface reduction during fixation and whereby the fixed tissue is highly resistant to calcification.