Patent ID: 6887466
Filing Date: 2005-05-03
Classification: A61K,C07K,C12N

Abstract:
1. A method for inducing ex vivo proliferation of a population of T cells to sufficient numbers for use in therapy, comprising contacting a population of T cells ex vivo with a surface having covalently attached thereto: (a) an anti-CD3 antibody or CD3-binding fragment thereof, which provides a primary activation signal to the T cells, thereby activating the T cells; and (b) an anti-CD28 antibody or CD28-binding fragment thereof, which stimulates a CD28 accessory molecule on the surface of the T cells, thereby stimulating the activated T cells, wherein the anti-CD3 antibody or CD3-binding fragment thereof, and the anti-CD28 antibody or CD28-binding fragment thereof, are covalently attached to the same surface, the anti-CD3 antibody or CD3-binding fragment thereof, and the anti-CD28 antibody or CD28-binding fragment thereof, thereby inducing the population of T cells to proliferate to sufficient numbers for use in therapy.