Patent ID: 8927256
Filing Date: 2015-01-06
Classification: C07K,C12N,C12P

Abstract:
1. A fermentation medium for production of a recombinant protein or a derivative or analog thereof by improving phosphate uptake through fermentation using a methanol inducible fungal species, said medium characterized in that a residual concentration of urea is maintained in a range of about 0.3M to about 1M, a methanol feeding rate ranges from about 6 g/L/h to about 20 g/L/h, basal salts per liter comprising: Phosphoric acid (85%): from about 2.67 ml to about 133.5 mL, Calcium sulfate: from about 0.093 g to about 4.65 g, Potassium sulfate: from about 1.82 g to about 91 g, Magnesium sulfate-7H Potassium hydroxide: from about 0.413 g to about 20.65 g, Glycerol: from about 4 g to about 200 g; and trace elements per liter comprising: Cupric sulfate.5H Sodium iodide: from about 0.008 g to about 0.4 g, Manganese sulfate-H Sodium molybdate-2H Boric Acid: from about 0.002 g to about 0.1 g, Cobalt chloride: from about 0.05 g to about 2.5 g, Zinc chloride: from about 2 g to about 100 g, Ferrous sulfate-7 H Biotin: from about 0.02 g to about 1 g, and Sulfuric Acid: from about 0.5 ml to about 25 ml.