Patent ID: 9096877
Filing Date: 2015-08-04
Classification: A61K,A61P,C07K,C12P

Abstract:
1. A method of attenuating the post-translational fucosylation of a molecule comprising a modified Fc region, comprising: modifying a human IgG Fc region to comprise an amino acid substitution relative to a wild type Fc region to yield a modified Fc region; expressing a candidate molecule comprising said modified Fc region in a host cell capable of mediating normal glycosylation; determining a ratio of high mannose oligosaccharide glycosylation to complex oligosaccharide glycosylation exhibited by said modified Fc region; identifying said candidate molecule comprising said modified Fc region as a suitable molecule if said modified Fc region exhibits said ratio that is greater than about 0.5 and less than about 5.5; and expressing said suitable molecule possessing said modified Fc region in said host cell.