Patent ID: 8394608
Filing Date: 2013-03-12
Classification: B01L,C12Q

Abstract:
1. A method of amplifying nucleic acids in a sample, comprising the steps of a. providing a container having b. injecting the sample into the container via a first of the sealable ports, and sealing the first port subsequent to injecting the sample, c. extracting the nucleic acids from the sample, and moving the extracted nucleic acids to the amplification blister, d. subjecting the nucleic acids in the amplification blister to amplification conditions, e. fluidly moving a portion of the nucleic acids to each of the additional second-stage amplification chambers, f. performing second-stage amplification in the additional second-stage amplification chambers, and wherein the extracting step includes lysing cells contained within the sample to generate a lysate, mixing nucleic acid-binding magnetic beads with the lysate and incubating to allow the nucleic acids to bind to the magnetic beads, deploying a magnet adjacent to one of the plurality of blisters to contain the magnetic beads in that blister, and moving the remainder of the lysate to another of the plurality of blisters.