Patent ID: 9051374
Filing Date: 2015-06-09
Classification: A61K,A61P,C07K

Abstract:
1. A method for producing an isolated monoclonal antibody or fragment thereof which specifically binds to a CD98 epitope, wherein the monoclonal antibody or fragment thereof: i. is secreted by a mammalian cell expressing a pair of antibody variable region polypeptides encoded by (a) SEQ ID NOs: 40 and 46, (b) SEQ ID NOs: 42 and 46, (c) SEQ ID NOs: 42 and 78, (d) SEQ ID NOs: 42 and 80, (e) SEQ ID NOs: 42 and 82, (f) SEQ ID NOs: 42 and 84, or (g) SEQ ID NOs: 28 and 30, said antibody variable region polypeptides being the only antibody variable region polypeptides expressed by said mammalian cell; or ii. is secreted by a mammalian cell expressing a pair of antibody variable region polypeptides encoded by a plasmid deposited under Accession Number FERM BP-10551 or a plasmid deposited under Accession Number FERM BP-10552, said antibody variable region polypeptides being the only antibody variable region polypeptides expressed by said mammalian cell; or iii. is secreted by a mammalian cell expressing a pair of antibody variable region polypeptides encoded by (a) a BglII-BsiWI fragment and a SalI-NheI fragment obtained from a plasmid vector C2IgG1/pCR4 deposited under Accession Number FERM BP-10551 or (b) a BglII-BsiWI fragment and a SalI-NheI fragment obtained from a plasmid vector K3/pCR4 deposited under Accession Number FERM BP-10552, said antibody variable region polypeptides being the only antibody variable region polypeptides expressed by said mammalian cell; or iv. is secreted by a mammalian cell expressing a pair of antibody variable region polypeptides encoded by a plasmid deposited under Accession Number FERM BP-10551, but wherein serine at position 97 of the heavy chain variable region is replaced with asparagine; phenylalanine at position 100 of the heavy chain variable region is replaced with serine; and isoleucine at position 117 of the light chain variable region is replaced with leucine, said antibody variable region polypeptides being the only antibody variable region polypeptides expressed by said mammalian cell, said method comprising: aa. obtaining a nucleic acid encoding said isolated anti-CD98 monoclonal antibody or fragment thereof; bb. introducing the nucleic acid into a host suitable for expression of said nucleic acid; cc. expressing said nucleic acid in said host; and dd. obtaining said isolated antibody or fragment thereof.