Patent ID: 6507788
Filing Date: 2003-01-14
Classification: C12N

Abstract:
A method for identifying a precursor of a peptide comprising an amidated C-terminal end comprising the steps of:(i) screening a nucleotide database for the presence of a Y1-Y2-Y3-Y4-Y5 sequence, wherein Y1 is a nucleotide sequence of 1 to 12 nucleotides or is suppressed, Y2 is a codon for Gly, Y3 and Y4 independently are codons for Arg or Lys, and Y5 is a nucleotide sequence of 1 to 21 nucleotides or Y5 is suppressed; (ii) identifying a polynucleotide sequence that comprises the Y1-Y2-Y3-Y4-Y5 sequence; (iii) determining a polypeptide encoded by the polynucleotide sequence to identify said precursor of the peptide comprising the amidated C-terminal end.