Patent ID: 7521593
Filing Date: 2009-04-21
Classification: C08B,C12N

Abstract:
1. A barley grain comprising; (i) starch, (ii) an exogenous nucleic acid molecule comprising a sequence which is the same as, or is fully complementary to, a nucleotide sequence of at least 50 contiguous nucleotides of the starch branching enzyme IIa (SBEIIa) gene coding region whose sequence is set forth in SEQ ID NO:1 which inhibits expression of a barley SBEIIa gene, (iii) a reduced level of SBEIIa protein and, (iv) a reduced level of starch branching enzyme IIb (SBEIIb) protein in the barley grain, the reduced level of SBEIIa protein and of SBEIIb protein being relative to untransformed barley grain of the Golden Promise variety, wherein the amylose content of the starch is at least 40% (w/w) of total starch content of the barley grain as measured by an iodometric method.