Patent ID: 8247207
Filing Date: 2012-08-21
Classification: A61K,A61P,C12N

Abstract:
1. A method for making a refrigerator-stable live cold-adapted influenza virus composition, comprising: (a) clarifying a viral harvest comprising live cold-adapted influenza viruses by filtration, thereby producing a clarified viral harvest filtrate; (b) subjecting the clarified viral harvest filtrate to continuous zonal centrifugation, thereby producing a further clarified viral harvest; (c) sterilizing by sterile filtration the further clarified viral harvest, thereby producing a sterilized viral harvest; and (d) combining the sterilized viral harvest with components of a stabilizer consisting of: thereby producing a refrigerator-stable live cold-adapted influenza virus composition, wherein each amount of each of the components of the stabilizer is the final amount in the virus composition, and which virus composition exhibits a potency loss of less than 1.0 log over a 12 month period when stored at 4° C. to 8° C.