Patent ID: 8519115
Filing Date: 2013-08-27
Classification: C12Q

Abstract:
1. A population of uniquely labeled nanoreporter probes, wherein each uniquely labeled nanoreporter probe comprises: i) a unique target-specific region; ii) a region comprising a unique, designed nanoreporter wherein said unique, designed nanoreporter comprises a single-stranded nucleic acid backbone, said single-stranded nucleic acid backbone comprising three or more label attachment regions covalently attached together in a unique linear combination, wherein each label attachment region of each single stranded nucleic acid backbone is different from the other label attachment regions in that same single stranded nucleic acid backbone, wherein the label attachment regions are selected from a population of designed polynucleotide sequences, and wherein the label attachment regions are selected from the group consisting of SEQ ID NOs: 1-24, wherein the designed polynucleotide sequence is hybridized to a complementary polynucleotide sequence having attached thereto one or more detectable molecules; wherein each complementary polynucleotide sequence has designated a specific detectable molecule; and wherein each uniquely labeled nanoreporter probe has a detectable signal that distinguishes it from other uniquely labeled nanoreporter probe in said population of uniquely labeled nanoreporter probes.