Patent ID: 8097413
Filing Date: 2012-01-17
Classification: C12Q

Abstract:
1. A method for detecting the presence or absence of GBS in a biological sample from an individual, said method comprising: performing at least one cycling step, wherein a cycling step comprises an amplifying step and a hybridizing step, wherein said amplifying step comprises contacting said sample with a pair of pts primers to produce a pts amplification product if a GBS pts nucleic acid molecule is present in said sample, wherein said pair of pts primers comprise a first pts primer and a second pts primer, wherein said first pts primer consists of the sequence 5′-TGA GAA GGC AGT AGA AAG CTT AG-3′ (SEQ ID NO:1), and wherein said second pts primer consists of the sequence 5′-TGC ATG TAT GGG TTA TCT TCC-3′ (SEQ ID NO:2), wherein said hybridizing step comprises contacting said sample with a pts probe, wherein said pts probe is selected from a sequence consisting of 5′-CAA ATT AAA GAG ACT ATT CGT GCA A-3′ (SEQ ID NO:3), a sequence consisting of 5′-CAA GTA AAT GCA GAA ACA GG-3′ (SEQ ID NO:4), and a sequence that permits secondary structure formation, wherein the pts probe is labeled with a donor fluorescent moiety and a corresponding acceptor fluorescent moiety; and detecting the presence or absence of fluorescence resonance energy transfer (FRET) between said donor fluorescent moiety and said acceptor fluorescent moiety of said pts probe, wherein the presence or absence of fluorescence is indicative of the presence or absence of GBS in said sample.