Patent ID: 8361753
Filing Date: 2013-01-29
Classification: C12Q

Abstract:
1. A method of amplifying nucleic acids, said method comprising: amplifying nucleic acid using a modified oligonucleotide primer, wherein said modified oligonucleotide primer comprises a modification group at one or two internucleotide linkages; wherein said modification groups are at the n, n-1, n-2, or n-3 positions, wherein n is the 3′ terminal internucleotide linkage; wherein the presence of said modification group impairs nucleic acid polymerase extension of said modified oligonucleotide primer; wherein said modification group can be non-reversibly thermally dissociated; and wherein said modified oligonucleotide primer has structure I as follows wherein: Nuc is a nucleoside within the primer sequence; U and Z are independently O, S, Se, NR R Y is O, S or Se; W is O, S, S(O), S(O) Q is a modification group comprising one or more thermally cleavable groups.