Patent ID: 8518392
Filing Date: 2013-08-27
Classification: A01K,C12N,C12Y

Abstract:
1. A targeting construct, comprising from 5′ to 3′: a. a mouse targeting arm; b. a first recombinase recognition site; c. a nucleic acid sequence encoding a selectable marker; d. a first promoter operably linked to a recombinase gene, wherein the recombinase gene encodes a recombinase that recognizes the first and the second recombinase recognition site, and wherein the first promoter is the Blimp1 promoter SEQ ID NO:2 or the Blimp1 promoter SEQ ID NO:3, and wherein the first promoter drives expression of the recombinase gene in differentiated cells and does not drive expression of the recombinase gene in undifferentiated cells; e. a second recombinase recognition site, wherein the first recombinase recognition site and the second recombinase recognition site are oriented to direct an excision; and, f. a mouse targeting arm.