Patent ID: 8574846
Filing Date: 2013-11-05
Classification: C12Q

Abstract:
1. A process comprising: (a) converting a single stranded DNA or RNA molecule into a double stranded nucleic acid molecule comprising a first and second strand of DNA; (b) contacting a recombinase agent selected from the group consisting of uvsX and recA with a first and second nucleic acid primer to form a first and second nucleoprotein primer; (c) contacting the first and the second nucleoprotein primer to said double stranded nucleic acid molecule thereby forming a first double-stranded structure at a first portion of said first strand and forming a second double stranded structure at a second portion of said second strand such that the 3′ ends of said first nucleic acid primer and said second nucleic acid primer are oriented toward one another on the same double-stranded template nucleic acid molecule; (d) extending the 3′ ends of said first and second nucleic acid primer with one or more polymerases and dNTPs to generate a first and second double-stranded nucleic acid and first and second displaced strands of nucleic acid; and (e) continuing the reaction through repetition of (c) and (d) until a desired degree of amplification is reached, wherein said process at steps (c) and (d) is performed in the presence of a crowding agent such that the crowding agent stimulates amplification.