Patent ID: 6294326
Filing Date: 2001-09-25
Classification: C12Q,Y10T

Abstract:
A method for detecting the presence of a target in a test sample comprising the steps of:(a) forming a reaction mixture comprising the test sample, a primer, a probe and amplification reagents wherein(i) the probe has a first and second label wherein the first label comprises a first specific binding member, and(ii) the primer and probe hybridize to the target such that the probe is downstream from the primer;(b) hybridizing the primer and probe to the target sequence and degrading the hybridized probe in a target dependent manner to separate the first and second labels;(c) contacting the reaction mixture with a capture reagent to form a first specific-binding-member/capture reagent complex;(d) detecting the second label associated with the first specific-binding-member/capture reagent complex, wherein a loss of signal indicates the presence of the target sequence;wherein said amplification reagents comprise an enzyme having 5'.fwdarw.3' exonuclease activity capable of degrading the hybridized probe only when the probe is hybridized to the target sequence.