Patent ID: 6875429
Filing Date: 2005-04-05
Classification: A61P,C12N,C12Q

Abstract:
1. A method of screening and recovering a regulatory DNA which is not expressed as an mRNA but is capable of inducing metastasis comprising the steps of: i. transferring fragments of human DNA of less than 1.5 kb in length, said fragments having been tagged at both ends with double-stranded synthetic oligonucleotides that provide restriction enzyme and unique primer sites, from malignant, metastatic cancer cells, into a rat or mouse cell line that produces only benign, non-metastasizing tumours when injected into a syngeneic rat or mouse, thereby producing transformed cells; ii. injecting the transformed cells into syngeneic rats or mice; iii. selecting those rats or mice in which metastasizing tumours have been identified; and iv. recovering the regulatory DNA capable of inducing metastasis therefrom.