Patent ID: 8445197
Filing Date: 2013-05-21
Classification: C07H,C07K,C12Q,G01N,Y10S

Abstract:
1. A method of identifying a sequence of a target polynucleotide, said method comprising: (a) providing a substrate comprising a plurality of discrete regions, wherein a majority of said plurality of discrete regions comprises a single concatemer comprising a plurality of monomeric units, wherein each monomeric unit comprises a first target sequence of said target polynucleotide and an adaptor, and said first target sequence is adjacent to said adaptor, and wherein said substrate is prepared by a photolithography method such that said plurality of discrete regions comprise a functional group for non-covalent attachment of said concatemer; (b) applying a first set of probes to said substrate, such that one or more probes from said first set hybridizes to said adaptor; (c) applying a second set of probes to said substrate, such that one or more probes from said second set hybridizes to said first target sequence; (d) ligating probes from said first set and probes from said second set that are hybridized to adjacent sequences of said monomeric unit to form a ligated complex; e) detected said ligated complex, thereby identifying a nucleotide of said target polynucleotide; (f) removing said ligated complex; (g) repeating steps (b) through (f) to determine said sequence of said target polynucleotide.