Patent ID: 6586223
Filing Date: 2003-07-01
Classification: C11D,C12N

Abstract:
A serine protease variant having a modified amino acid sequence of a wild-type amino acid sequence, the wild-type sequence comprising a first epitope region and a second epitope region, wherein the modified amino acid sequence comprises a substitution by a substituting amino acid at one or more positions in one or more of the epitope regions, wherein:(a) when a substitution occurs in the first epitope region, the substitution occurs at a position corresponding to position 125 of the amino acid sequence of subtilisin BPNâ€² set forth in SEQ ID NO:1, wherein: (i) when a substitution occurs at position 125, the substituting amino acid is selected from the group consisting of Ala, Cys, Asp, Glu, Phe, Gly, His, Ile, Lys, Leu, Met, Asn, Pro, Gln, Arg, Thr, Val, Trp, and Tyr; and (b) when a substitution occurs in the second epitope region, the substitution occurs at a position corresponding to position 225 of the amino acid sequence of subtilisin BPNâ€² set forth in SEQ ID NO:1, wherein: (i) when a substitution occurs at position 225, the substituting amino acid is selected from the group consisting of Cys, Asp, Phe, Gly, His, Ile, Asn, Thr, Trp, and Tyr.