Patent ID: 7276339
Filing Date: 2007-10-02
Classification: A01K,C07K

Abstract:
1. A process involving competitive binding for identifying a chemical compound which specifically binds to a human NPFF1 receptor which comprises separately contacting cells expressing on their cell surface the human NPFF1 receptor, or a membrane preparation of such cells, wherein such cells prior to being transfected with DNA encoding the human NPFF1 receptor do not express the human NPFF1 receptor, with both the chemical compound and a second chemical compound known to bind to the receptor, and with only the second chemical compound, under conditions suitable for binding of both compounds, and detecting specific binding of the chemical compound to the human NPFF1 receptor, a decrease in the binding of the second chemical compound to the human NPFF1 receptor in the presence of the chemical compound indicating that the chemical compound binds to the human NPFF1 receptor, wherein the human NPFF1 receptor has an amino acid sequence identical to the amino acid sequence shown in 1) SEQ ID NO: 8 or 2) that encoded by plasmid pcDNA3.1-hNPFF1 (ATCC Accession No. 203605).