Patent ID: 6246470
Filing Date: 2001-06-12
Classification: G01N

Abstract:
The method for determination in an analyzed liquid of a biologically active substance interacting with a lyotropic cholesteric liquid-crystalline DNA dispersion formed in a polymer neutral in respect to DNA, distinguished by:that the lyotropic cholesteric liquid-crystalline dispersion is formed in an aqueous-salt solution of the specified polymer of the linear double-stranded DNA molecules of a low molecular mass immediately before mixing with the analyzed liquid containing the determined biologically active substance, thereat the analysed liquid is previously mixed with the specified polymer under conditions, when the optical properties of the lyotropic liquid-crystalline DNA dispersion are not broken, then through the analysed sample, obtained as a result of mixing of the prepared analyzed liquid with the specified liquid-crystalline DNA dispersion, a flow of circular-polarized light is passed, and the optical signal is registered generated by the liquid-crystalline dispersion at two wavelengths, one of them is in the region of the DNA absorption, and another one is in the region of absorption of the determined biologically active substance, then the ratio is calculated between these signals at the specified wavelengths, and the concentration of the biologically active substance is determined on this ratio using the calibration curve.