Patent ID: 8716512
Filing Date: 2014-05-06
Classification: A61P,B01D,C07B,C07F,C07K

Abstract:
1. A method of preparing a recombinant lipoprotein from E. coli cells, comprising disrupting cellular membranes of filtering the cell membrane fraction to generate a filtrate; purifying the filtrate by a purification process to obtain a sample, the purification process comprising, in any order, mixed phase chromatography in a resin, cation membrane chromatography, and anion membrane chromatography; diafiltrating the sample with a diafiltration membrane to generate a preparation; and removing the detergent from the preparation to obtain purified Ag473 protein, wherein the resin is an activated silane-modified silica resin prepared by a process comprising: (1) mixing in a solvent a silica resin and silane, the ratio of the silica resin to the silane ranging from about 2.5 to about 3.5, calculated based on dry weight; (2) incubating the mixture so that the silica resin and the silane react with each other to form a silane-modified silica resin; (3) removing the solvent to obtain a dried silane-modified silica resin; (4) activating the silane-modified silica resin by washing it with an acetic acid solution; (5) removing the acetic acid solution; (6) and washing the activated silane-modified silica resin with a buffer.