Patent ID: 6630317
Filing Date: 2003-10-07
Classification: C07K,C12N,C12Q,C40B

Abstract:
A method for increasing the expression of a heterologous periplasmic protein in functional form in a bacterial host cell, characterized by co-expressing said periplasmic protein and a (poly)peptide identified by:(a) providing a collection of host cells wherein each cell comprises (i) a first nucleic acid sequence out of a collection of nucleic acid sequences, and (ii) a second nucleic acid sequence encoding said heterologous periplasmic protein; (b) causing or allowing expression of (i) (poly)peptides expressible from said collection of nucleic acid sequences, and (ii) said heterologous periplasmic protein expressible from said second nucleic acid sequence; (c) screening or selecting for a host cell expressing said heterologous periplasmic protein with increased functional yield, wherein the polypeptide encoded by said first nucleic acid sequence and expressed in the screened or selected host cell enhances the folding of said periplasmic protein; (d) optionally, repeating step (c) one or more times; (e) obtaining said first nucleic acid sequence contained in said host cell; and (f) deducing a (poly)peptide sequence therefrom.