Patent ID: 6342373
Filing Date: 2002-01-29
Classification: A61K,C07K

Abstract:
A process for the preparation of an eglin compound having the following N-acetyl amino acid sequence:N-acetyl-Thr-Glu-Phe-Gly-Ser-Glu-Leu-Lys-Ser-Phe-Pro-Glu-Val-Val-Gly-Lys-Thr-Val-Asp-Gln-Ala-Arg-Glu-Tyr-Phe-Thr-Leu-His-Tyr-Pro-Gln-Tyr-Asp-Val-W-Phe-Leu-Pro-Glu-Gly-Ser-Pro-Val-Thr-Leu-Asp-Leu-Arg-Tyr-Asn-Arg-Val-Arg-Val-Phe-Tyr-Asn-Pro-Gly-Thr-Asn-Val-Val-Asn-His-Val-Pro-His-Val-Gly (Formula XIVâ€²) in which W is Tyr or His, said process comprising:a) transforming host cells of Escherichia coli or Saccharomyces cerevisiae with an expression vector, said expression vector comprising a promoter of host cell origin and a DNA (sequence coding for said eglin compound, wherein said DNA sequence is directly and operably linked to and in proper reading frame relative to said promoter, in a liquid medium containing assimilable sources of carbon and nitrogen, b) culturing the transformed host cells in a liquid medium containing assimilable sources of carbon and nitrogen suitable for expression of said expression vector, and c) isolating said eglin compound.