Patent ID: 6472206
Filing Date: 2002-10-29
Classification: C12Q,G01N

Abstract:
A method for the detection of at least one intracellular parasite in a sample, comprising the steps of:a) providing: i) a cell culture comprising cells, wherein said cell culture has been frozen and thawed in situ on a substrate, wherein the in situ frozen and thawed cells detect at least one intracellular parasite, and ii) a sample suspected of containing at least one intracellular parasite; b) adding said sample to said cell culture, to produce an inoculated culture; c) incubating said inoculated culture under conditions such that said intracellular parasite infects said cells of said cell culture to produce an infected culture; and d) observing said infected culture for the presence of said intracellular parasite within the cells of said cell culture.