Patent ID: 7109295
Filing Date: 2006-09-19
Classification: C07K,C12N

Abstract:
1. A fusion polypeptide that regulates a target gene, consisting of: a first portion consisting of one or more zinc finger monomers comprising an amino acid sequence that binds to a nucleic acid recognition sequence in the target gene; a second portion consisting of a dimerization domain of a tetracycline repressor linked to the first portion such that the one or more zinc finger monomers can bind to the recognition sequence in the target gene, wherein the second portion is optionally linked to the first portion via a junction sequence; and a third portion consisting of an accessory domain that modulates a promoter operably linked with the target gene, wherein the accessory domain is linked to the zinc finger monomer such that the accessory domain is localized to the promoter when the zinc finger monomer binds to the recognition sequence, wherein the third portion is optionally linked to the first portion via a flexible junction sequence.