Patent ID: 7384763
Filing Date: 2008-06-10
Classification: C07H,C12Q,G01N,Y02A

Abstract:
1. A method of detecting β-D-ribofuranosidase activity on a solid medium including the steps: a) contacting a chromogenic β-D-ribofuranoside comprising a β-D-ribofuranosyl group and a chromogenic portion, with a sample comprising bacteria suspected of containing β-D-ribofuranosidase activity, on a solid growth medium; b) incubating the mixture on the growth medium under conditions such that bacterial growth occurs; and c) determining whether an indicator is formed in the solid medium, and d) correlating whether an indicator is formed in step c) with β-D-ribofuranosidase activity, wherein the chromogenic portion cleaved by β-D-ribofuranosidase from the β-D-ribofuranosyl group to release a chromogenic product and form the indicator which is or is formed from the chromogenic product and is substantially non-diffusible in the solid medium.