Patent ID: 8227187
Filing Date: 2012-07-24
Classification: C12Q,Y10T

Abstract:
1. An assay method for detecting polynucleotide binding comprising: a. providing a sample that is suspected of containing a first polynucleotide; b. providing a second polynucleotide that is complementary to the first polynucleotide; c. providing a signaling chromophore; d. providing a conjugated polymer that upon excitation is capable of transferring energy to the signaling chromophore; e. contacting the sample with the second polynucleotide, the signaling chromophore and the conjugated polymer under conditions in which the first and second polynucleotides can hybridize to form a signaling complex further comprising the conjugated polymer and the signaling chromophore; and f. determining if the first and second polynucleotides have hybridized by detecting if light is produced from the signaling chromophore upon excitation of the conjugated polymer.