Patent ID: 7521243
Filing Date: 2009-04-21
Classification: A61B,G01N,Y10T

Abstract:
1. A method for volumetric enumeration of white blood cells in a blood sample, said method comprising: acquiring a blood sample into a measurement cavity of a sample acquiring device, said measurement cavity holding a reagent comprising a hemolysing agent and a staining agent to react with the sample such that the white blood cells are stained, irradiating the sample with the stained white blood cells, acquiring a digital image of a magnification of the irradiated sample in the measurement cavity, wherein white blood cells are distinguished by selective staining of the staining agent, and digitally analyzing the digital image for identifying white blood cells and determining the number of white blood cells in the samples, wherein said digital image is acquired with a depth of field at least corresponding to a third of the thickness of the measurement cavity.