Patent ID: 6344356
Filing Date: 2002-02-05
Classification: C07K,C12N,C12Q,C12Y,C40B,H01L

Abstract:
A method of recombining nucleic acids, the method comprising:producing fragments of at least two corresponding nucleic acids, wherein at least one of the at least two nucleic acids comprises a codon-altered nucleic acid; hybridizing the fragments to form hybrid fragments; elongating the resulting hybrid fragments by conducting a multi-cyclic polynucleotide extension process on the hybrid nucleic acids under conditions whereby one nucleic acid strand serves as a template for extension of another nucleic acid strand with which it is partially annealed, to generate a population of shuffled polynucleotides; and selecting or screening the population of shuffled polynucleotides for one or more desired characteristics, thereby identifying at least one selected recombinant nucleic acid.