Patent ID: 7972802
Filing Date: 2011-07-05
Classification: G01N

Abstract:
1. A method of screening a mammalian subject to determine if the subject has a predisposition to develop, or is suffering from, cardiovascular disease, the method comprising (a) isolating a high density lipoprotein subfraction in the density range of from 1.06 g/mL to 1.210 g/mL from a biological sample isolated from the subject; (b) enzymatically digesting at least a portion of the subfraction isolated in step (a) into peptides; (c) detecting a biomarker profile comprising at least two biomarkers present in the high density lipoprotein subfraction digested in accordance with step (b), wherein the at least two biomarkers comprise at least two peptide fragments comprising 6 continuous amino acids to 20 continuous amino acids from SEQ ID NOS:1-14, or naturally occurring derivatives thereof that are at least 90% identical to SEQ ID NOS:1-14; and (d) comparing the biomarker profile detected in accordance with step (c) to a reference profile, wherein a difference in the amount of the at least two biomarkers between the high density lipoprotein subfraction of the biological sample and the reference profile is indicative of the presence and/or risk of developing cardiovascular disease.