Patent ID: 6333150
Filing Date: 2001-12-25
Classification: C12Q

Abstract:
A method for the detection of dengue virus RNA, comprising:a) obtaining a sample which may contain dengue virus RNA;b) performing an isothermal transcription based amplification on the sample with two oligonucleotide primers, a first primer which comprises at least 10 consecutive nucleotides of a sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:6 and SEQ ID NO:7, and a second primer which comprises at least 10 consecutive nucleotides of a sequence selected from the group consisting of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:8 and SEQ ID NO:9; andc) detecting the amplification product using a labeled probe, whereby hybridization of the probe to the amplification product indicates the presence of dengue virus RNA in the sample.