Patent ID: 8691527
Filing Date: 2014-04-08
Classification: A61P,C07K,C12N,C12P

Abstract:
1. A recombinant gene expression modulation system comprising a gene expression cassette that is capable of being expressed in a host cell comprising a polynucleotide that encodes a polypeptide comprising: i) a transactivation domain; ii) a DNA-binding domain that recognizes a response element associated with a gene whose expression is to be modulated; and iii) a spruce budworm (a) amino acid residue 52 of SEQ ID NO: 1, (b) amino acid residue 55 of SEQ ID NO: 1, (c) amino acid residue 96 of SEQ ID NO: 1, (d) amino acid residue 107 of SEQ ID NO: 1, or (e) amino acid residue 175 of SEQ ID NO: 1; a double substitution mutation at (f) amino acid residues 107 and 175 of SEQ ID NO: 1, (g) amino acid residues 107 and 127 of SEQ ID NO: 1, (h) amino acid residues 127 and 175 of SEQ ID NO: 1; or a triple substitution mutation at (i) amino acid residues 52, 107 and 175 of SEQ ID NO: 1, (j) amino acid residues 96, 107 and 175 of SEQ ID NO: 1, or (k) amino acid residues 107, 127 and 175 of SEQ ID NO: 1, wherein said recombinant gene expression modulation system exhibits increased activity in response to a steroidal ligand or a non-steroidal ligand, relative to a gene expression modulation system that contains said spruce budworm