Patent ID: 7763459
Filing Date: 2010-07-27
Classification: A61F,A61L

Abstract:
1. A method of decellularizing a tissue by sequential extraction, comprising: treating isolated tissue with a first solubilizing fluid comprising at least one oxidizing agent at a concentration effective to extract cellular and nuclear material from the tissue, the first solubilizing fluid further comprising at least one of a reactive hydroxyl radical generating agent and a reactive oxygen generating specie; treating the isolated tissue with a second solubilizing fluid before treating the isolated tissue with the first solubilizing fluid, the second solubilizing fluid comprising at least one oxidizing agent, to remove cellular and nuclear material from the isolated tissue, the at least one oxidizing agent of the second solubilizing fluid being different than the at least one oxidizing agent of the first solubilizing fluid, the second solubilizing fluid further comprising a peroxy acid; and washing the isolated tissue in a washing fluid to remove cellular debris, the method substantially maintaining the biochemical composition and the mechanical and biological properties of an extracellular matrix of the isolated tissue, the method resulting in the extracellular matrix of the tissue being at least about 60% substantially free of viable and non-viable nucleic acids.