Patent ID: 6077679
Filing Date: 2000-06-20
Classification: A61K,C07K,C12N,C12Q,G01N,Y10S

Abstract:
A kit for measuring activity of a herpes virus proteinase, comprising:a proteinase encoded by a herpes virus; anda substrate for cleavage by said proteinase, said substrate comprising a polypeptide containing an amino acid sequence selected from the group consisting of aa.sub.1 -aa.sub.2 -Ala-aa.sub.3 (SEQ ID NO:31) and Tyr-aa4-aa.sub.5 -Ala-aa.sub.6 (SEQ ID NO:33), wherein aa.sub.1 is Val or Leu, aa.sub.2 is a polar amino acid, aa.sub.3 is Ser, Val, or Asn, aa.sub.4 is Val or Leu, aa.sub.5 is Lys or Gln and aa.sub.6 is Ser or Asn, wherein said proteinase cleaves said substrate on the carboxy terminal side of the Ala residue, said kit being substantially free of intact infectious herpes virus.