Patent ID: 6303322
Filing Date: 2001-10-16
Classification: B01L,C07K,C12Q,C40B,G01N,Y02A

Abstract:
In a combinatorial method for generating lead compounds which comprises synthesizing a multiplicity of compounds and testing the ability of said compounds to bind to a receptor molecule, the improvement comprising(a) contacting said receptor molecule with one compound of said multiplicity of compounds in each of a multiplicity of wells in a microplate;(b) simultaneously heating said multiplicity of wells from step (a);(c) measuring in each of said wells a physical change associated with the thermal unfolding of said receptor molecule resulting from said heating;(d) generating a thermal unfolding curve for said receptor molecule as a function of temperature for each of said wells;(e) comparing each of said thermal unfolding curves in step (d) with the thermal unfolding curve obtained for said receptor molecule in the absence of any of said compounds in said multiplicity of different compounds; and(f) ranking the affinities of said multiplicity of different compounds for said receptor molecule according to the change in each of said thermal unfolding curves.