Patent ID: 6255559
Filing Date: 2001-07-03
Classification: A01H,C12N

Abstract:
A method for producing genetically modified plant material comprising:preparing an in vitro shoot culture of a target plant, the target plant being of the Eucalyptus or Pinus species;maintaining and growing the shoot culture until it has produced multiple modes and stem segments;selecting and excising the stem segments from one or more nodes of a shoot;transforming a stem segment by stably incorporating a genetic contruct comprising a selection marker and a polynucleotide of interest into the stem segment to form a putatively transformed stem segment;exposing a putatively transformed stem segment to a selection medium comprising a selection agent that permits survival of transformed stem segments and is lethal to stem bud on the that were not successfully transformed;selectively inducing the formation of an adventitious bud on the putatively transformed stem segment to form a putatively transformed adventitious bud;selectively regenerating the putatively transformed adventitious bud by excising the putatively transformed adventitious bud and exposing the putatively transformed adventitious bud to a selection medium comprising a selection agent that permits survival of transformed adventitious buds and is lethal to adventitious buds that were not successfully transformed to identify a transformed adventitious bud; andelongating the transformed adventitious bud to form a transformed shoot.