Patent ID: 8735170
Filing Date: 2014-05-27
Classification: G01N

Abstract:
1. A method for measuring a concentration of a first antigen contained in a test solution, by using an apparatus for measuring a concentration of a biogenic substance, the method comprising the following steps (a) to (h): step (a) of preparing the apparatus, the apparatus comprising: step (b) of supplying the test solution to the space for retaining the test solution to allow the first antigen to be bound to the first antibody and not to allow the first antigen to be bound to the blocking substance; step (c) of allowing polarized light parallel to the long-axis direction of the plurality of first metallic nanorods to pass through the cell along the optical axis to receive resultant first light with the photoreceiver; step (d) of calculating an amount Δλa of shift of a localized surface plasmon resonant wavelength caused by binding the first antigen to the antibody, on the basis of the first light; step (e) of rotating at least either one of the light source or the cell such that the polarized light is parallel to the long-axis direction of the plurality of second metallic nanorods; step (f) of allowing polarized light parallel to the long-axis direction of the plurality of second metallic nanorods to pass through the cell along the optical axis to receive resultant second light with the photoreceiver; step (g) of calculating an amount Δλb of shift of the localized surface plasmon resonant wavelength caused by supplying the test solution to the space for retaining the test solution, on the basis of the second light; and step (h) of calculating the concentration of the first antigen on the basis of a calibration curve and a difference represented by the following equation: