Patent ID: 7186521
Filing Date: 2007-03-06
Classification: A01K,C07K,C12N,G01N

Abstract:
1. A method for determining the effect of a substance on sequestration, uptake or accumulation of amyloid in brain cells, said method comprising: (A) exposing brain cells in vitro to an integrin antagonist, wherein said antagonist is selected from the group consisting of function blocking anti-β5 subunit integrin antibody, function blocking anti-β1 subunit integrin antibody, an RGD peptide capable of modifying integrin adhesion, RGDS peptide, GRGDS peptide, GRGDSP peptide, GRGDTP peptide and echistatin, (B) maintaining said cells for a time sufficient to induce sequestration, uptake or accumulation of amyloid in said cells as a result of said antagonist, (C) adding said substance before, during and/or after said exposing or maintaining; and (D) determining whether the presence of said substance has an effect on said antagonist induced sequestration, uptake or accumulation of amyloid.