Patent ID: 8071388
Filing Date: 2011-12-06
Classification: C07D,C07H,C09B,G01N,Y10T

Abstract:
1. An improved in vitro method for detecting a target analyte having two or more binding sites via nucleic acid-templated chemistry, wherein the improvement comprises: a) detecting the target analyte by producing a hemicyanine dye having the chemical formula: via an aldol condensation between an aldehyde having the chemical formula: and an active hydrogen component having the chemical formula: in an aqueous condition at room temperature in the presence of an organocatalyst wherein: Z′ is O, S, Se, P, NH n is 0, 1, or 2; R is H or alkyl; R′ is phenyl, N-heterocycle, H, alkyl, —SO or —O-alkyl; R″ is a linked nucleic acid; R′″ is a linked nucleic acid; and the organocatalyst is a secondary amine, a primary amine, or a bifunctional amine-acid catalyst, and b) detecting the hemicyanine dye, wherein production of the hemicyanine dye is indicative of the presence of the target analyte.