Patent ID: 7514223
Filing Date: 2009-04-07
Classification: A61P,C07K,G01N

Abstract:
1. A method of enriching a pool of analyte molecules with candidate analyte molecules that selectively bind a target molecule, comprising: (a) determining electrochemiluminescence assay (ECLA) responses for individual members of a pool of analyte molecules binding a target molecule; (b) applying a detection limit to the analysis of the ECLA responses, wherein an ECLA response equal to or greater than the ECLA detection limit identifies an electrochemiluminescence assay positive (ECLA (c) determining immunoassay (IA) responses for individual members of the pool of analyte molecules binding the target molecule; (d) applying a detection limit to the analysis of the IA responses, wherein an IA response equal to or greater than the IA detection limit is immunoassay positive (IA (e) generating a pool of candidate analyte molecules comprising: (f) confirming specific binding affinity of an analyte molecule selected from the enriched pool of candidate analyte molecules.