Patent ID: 6573046
Filing Date: 2003-06-03
Classification: C12N

Abstract:
A method of transforming a target DNA sequence into a different, desired sequence in a eukaryotic cell that comprises:(A) administering to the cell a chimeric duplex mutational vector comprising: a. a first oligonucleobase strand of at least 12 linked nucleobases and not more than 75 linked nucleobases, which strand has a first terminal and a second terminal nucleobase; b. a second oligonucleobase strand having a 3â€² most nucleobase and a 5â€² most nucleobase and having a number of nucleobases equal to the first strand, which second strand is divided into a first chain and a second chain; and c. a 3â€² end nucleobase and a 5â€² end nucleobase; in which i. the 3â€² most and 5â€² most nucleobases of the second strand are Watson-Crick base paired to the first terminal and the second terminal nucleobase of the first strand, respectively, ii. the nucleobases of the first chain are deoxy-type nucleobases and nucleobases of the first strand paired therewith are nuclease resistant ribo-type nucleobases; iii. the second strand contains at least two non-overlapping regions of at least 5 contiguous nucleobases that are Watson-Crick base paired to nucleobases of the first strand, â€ƒwherein the sequence of a strand comprises the sequence of the target DNA and the sequence of a strand comprises the sequence of the different, desired sequence and the oligonucleobase segment having said different, desired sequence is comprised of at least 12 contiguous deoxyribo-type nucleobases; and (B) detecting the presence in the cell or the progeny thereof of the DNA having the different, desired sequence.