Patent ID: 8338185
Filing Date: 2012-12-25
Classification: C12N,C12Q,Y10T

Abstract:
1. A method of determining relative potency of a chemically-synthesized RNAi agent comprising: forming a first test sample comprising the chemically-synthesized RNAi agent and Ago, wherein the first test sample is not in a cell; forming a second test sample comprising an RNAi control agent and Ago2, wherein the second test sample is not in a cell; and measuring the Ago2 activity of the chemically-synthesized RNAi agent and the Ago2activity of the RNAi control agent, wherein the difference in the Ago2 activity of the chemically-synthesized RNAi agent and Ago2 activity of the control RNAi agent indicates potency of the chemically-synthesized RNAi agent relative to the control RNAi agent.