Patent ID: 7037653
Filing Date: 2006-05-02
Classification: C12Q

Abstract:
1. A method for obtaining and amplifying extracted DNA from gum samples comprising one or more of guar gum (E 412) and locust bean gum (E 410), comprising the steps of: i) contacting a gum sample comprising DNA and one or more of guar gum (E 412) and locust bean gum (E 410) with an aqueous solution to form an extraction mixture; ii) agitating the extraction mixture at a temperature between 0° C. and 100° C. for a time period sufficient to permit extraction of DNA from the gum sample into the aqueous solution; iii) separating the extraction mixture to obtain an aqueous solution containing extracted DNA and another phase; iv) recovering a sample of the aqueous solution containing extracted DNA; and v) using PCR to amplify the extracted DNA using one or more primers having a sequence of SEQ ID NO:5, SEQ ID NO:6.