Patent ID: 7893319
Filing Date: 2011-02-22
Classification: C07K,C12N

Abstract:
1. A method of maintaining a homozygous recessive condition of a male sterile plant, the method comprising: (a) providing a first plant comprising homozygous recessive alleles of the Ms26 gene wherein said plant is male sterile, (b) introducing a construct into a second plant, the second plant comprising homozygous recessive alleles of the Ms26 gene, the construct in the hemizygous condition, the construct comprising: (iii) a third nucleotide sequence operably linked to the first nucleotide sequence, said third nucleotide sequence directing expression preferentially to male plant cells and selected from the group consisting of SEQ ID NO: 20 and a functional fragment of SEQ ID NO: 20, which functional fragment is essential for male tissue preferred expression of a linked sequence; and (c) fertilizing the first plant with the male gametes of the second plant to produce progeny which maintain the homozygous recessive condition of the first plant.