Patent ID: 9029105
Filing Date: 2015-05-12
Classification: C07C,C12N,C12P

Abstract:
1. A method for producing L-methionine and organic acid, which comprises the following steps: i) producing L-methionine precursor in a fermentation medium by the fermentation of an L-methionine precursor-producing microbial strain, wherein the L-methionine precursor-producing microbial strain is prepared by deleting the activity of cystathionine gamma synthase or O-succinylhomoserine sulfhydrylase or O-acetylhomoserine sulfhydrylase involved in the degradation of L-methionine precursor and by enhancing the activity of homoserine O-succinyl transferase or homoserine O-acetyl transferase involved in L-methionine precursor synthesis from homoserine; and ii) producing L-methionine and organic acid outside of the L-methionine precursor-producing microbial strain by the enzyme reaction using the L-methionine precursor and methylmercaptan or its salts as substrates, wherein the enzyme reaction is performed by other microbial strains containing the activity of cystathionine gamma synthase or O-succinylhomoserine sulfhydrylase or O-acetylhomoserine sulfhydrylase, wherein the L-methionine precursor is O-acetylhomoserine or O-succinylhomoserine.