Patent ID: 7033763
Filing Date: 2006-04-25
Classification: C12Q

Abstract:
1. A method of detecting a nucleic acid which comprises: (a) annealing to a nucleic acid a first oligonucleotide P*, wherein the first oligonucleotide P* has a non-extendable 3′ end, wherein the 3′ non-extendable terminus of first oligonucleotide P* is removable by pyrophosphorolysis; (b) removing the 3′ non-extendable terminus of the first oligonucleotide P* by pyrophosphorolysis to produce an unblocked first oligonucleotide; (c) extending the unblocked first oligonucleotide; (d) detecting the presence of the nucleic acid by detecting the extended first oligonucleotide.