Patent ID: 9024000
Filing Date: 2015-05-05
Classification: C07K

Abstract:
1. A method of purifying a target protein using a chromatography apparatus consisting of two columns including a first column and a second column, the method comprising: 1) loading a sample on the first column; 2) loading an unabsorbed fluid from the first column, on the second column which is connected to the first column in series; 3) after injecting the sample on the first column to the maximum static binding capacity (qm) of the first column, loading the sample on the second column while the target protein is collected from the fist column and the first column is regenerated in a state of being disconnected from the second column; 4) after the regeneration of the first column in step 3) is completed, loading an unabsorbed fluid from the second column on the first column by connecting the second column to the first column in series; 5) after injecting the sample on the second column to the maximum static binding capacity (qm) of the second column, loading the sample on the first column while the target protein is collected from the second column and the second column is regenerated in a state of being disconnected from the first column; and 6) performing the steps 1) to 5) repetitively until the complete consumption of the sample.