Patent ID: 6303382
Filing Date: 2001-10-16
Classification: A01N,C07K,C12N

Abstract:
A method for obtaining an integrant of Bacillus thuringiensis which has greater pesticidal activity than a corresponding parental strain by producing a larger quantity of a crystal delta-endotoxin as compared to said corresponding parental strain wherein the crystal delta-endotoxin produced by the integrant Bacillus thuringiensis has an activity directed towards the same pest as the crystal delta-endotoxin produced by the corresponding parental strain comprising(a) introducing into a cell of a parental strain a DNA construct lacking a Bacillus thuringiensis origin of replication comprising (i) a DNA sequence encoding a delta-endotoxin, wherein said delta-endotoxin is the same delta-endotoxin as the parental strain delta-endotoxin, (ii) a DNA sequence which is homologous with a region of the genome of said cell; and (iii) a selectable marker; and(b) integrating the introduced DNA construct of step (a) into the genome of the cell of said parental strain by homologous recombination in the presence of a selecting agent to obtain an integrant.