Patent ID: 6255046
Filing Date: 2001-07-03
Classification: A61K,A61P,C12N,C12Y

Abstract:
A method for screening for a candidate therapeutic agent that inhibits kinase enzymatic activity of iPFK-2, comprising:(a) expressing an iPFK-2 enzyme or kinase domain thereof from the cDNA sequence of SEQ ID NO. 11;(b) purifying and isolating the expressed iPFK-2 enzyme or kinase domain thereof;(c) providing a test compound that is a candidate therapeutic agent and fructose 6-phosphate substrate; and(d) measuring formation of fructose 2,6-bisphosphate product as a measure of the kinase enzymatic activity, wherein a reduction of kinase enzymatic activity compared to a control assay that does not include said test compound identifies a candidate therapeutic agent.