Patent ID: 8710211
Filing Date: 2014-04-29
Classification: C07C,C07H,C08G,C12N,C12Q

Abstract:
1. A method for isolating polynucleotides from a cell-containing sample in a process tube, the method comprising: contacting the sample in the process tube with a lysis solution having a pH between 4 and 8, thereby releasing polynucleotides from the cells in the cell-containing sample; contacting the sample in the process tube with a plurality of magnetic binding particles coated in PAMAM, wherein the polynucleotides become reversibly bound to the PAMAM, thereby creating binding particles bound with polynucleotides and a solution containing residual cellular material in the process tube; compacting the binding particles bound with the polynucleotides in the process tube by positioning a magnet in communication with the binding particles; removing the solution containing residual cellular material from the process tube; washing the binding particles with a solution having a pH≦9, thereby retaining the polynucleotides bound to the binding particles; and contacting the binding particles bound with polynucleotides with a solution having a pH ≧9 to release the polynucleotides from the PAMAM on the binding particles, thereby isolating the polynucleotides from the cell-containing sample.