Patent ID: 7906293
Filing Date: 2011-03-15
Classification: G01N,Y10T

Abstract:
1. A method of detecting an analyte in a test sample, the method comprising the steps of: (a) contacting the test sample containing the analyte with a specific binding partner to form an analyte specific binding partner complex; (b) separating the analyte specific binding partner complex from the test sample to form an analyte specific binding partner complex sample; (c) adding at least one analyte-specific enzyme to the analyte specific binding partner complex sample to produce peroxide; (d) adding an acridinium-9-carboxylate aryl ester to the analyte specific binding partner complex sample, the acridinium-9-carboxylate aryl ester having a formula of: (e) adding a basic solution to the analyte specific binding partner complex sample to generate a light signal, wherein the light signal is generated in a basic solution with a pH greater than or equal to 10; and (f) quantifying the amount of analyte in the analyte specific binding partner complex sample by relating the amount of light generated in the test sample by comparison to a standard curve for said analyte.