Patent ID: 8114656
Filing Date: 2012-02-14
Classification: C11D,C12N,C12Y

Abstract:
1. An isolated variant Bacillus neutral metalloprotease having at least 95% amino acid sequence identity to the parental polypeptide of SEQ ID NO: 18, and comprising: (i) a substitution at least one of the positions selected from the group consisting of S23, Q45, T59, S66, S129, F130, M138, V190, S199, D220, K211, and G222, with respect to the parental polypeptide, or (ii) a substitution selected from the group consisting of Q45E, T59P, S66E, S129I, S129V, F130L, M138I, V190I, S199E, D220P, D220E, K211V, K214Q, G222C, T004V/S023N, T059K/S66Q/S129I, T059R/S66N/S129I, and T059K/S66Q/S129V, wherein the variant has improved thermostability compared to the parental polypeptide.