Patent ID: 7566573
Filing Date: 2009-07-28
Classification: G01N

Abstract:
1. A method for determining the amount of an analyte in a sample comprising: a. providing a quantity of a first specific binding partner for the analyte, b. labeling a first aliquot of the quantity of the first specific binding partner with a chemiluminescent label, c. labeling a second aliquot of the quantity of the first specific binding partner with a fluorescent label, d. mixing the first and second aliquots with the sample, e. detecting an amount of association of the fluorescent label with the analyte, detecting an amount of association of the chemiluminescent label with the analyte, wherein detecting the amount of the association of the fluorescent label with the analyte comprises comparing the signal from the fluorescent label associated with the analyte to a standard curve of the concentration of the analyte versus a normalized fluorescence signal, and f. determining the amount of the analyte in the sample by correlating the amount of association of the fluorescent label with the analyte and the amount of association of the chemiluminescent label with the analyte to the amount of the analyte in the sample.