Patent ID: 9097718
Filing Date: 2015-08-04
Classification: C12Q,G01N,Y10T

Abstract:
1. A method for detecting a multi-stranded nucleic acid, comprising a step of bringing a chromogenic leuco dye into contact with a multi-stranded nucleic acid, wherein a color formed by interaction between the leuco dye and the multi-stranded nucleic acid is detected under visible light, and wherein the leuco dye is a compound represented by General Formula (I): wherein R 1 , R 2 and R 3 each independently represent a substituted or unsubstituted aryl group, and L represents —SO 3 R 4 , —NO 3 ,—NO 2 , —CN, —X, —NHR 5 , —N(COR 6 )(COR 7 ), —SR 8 , —SSR 9 , —OR 10 , —NHSNH 2 , —OH or —H, with R 4 representing an alkali metal or a hydrogen atom; X representing a halogen atom; and R 5 , R 6 , R 7 , R 8 , R 9 and R l0 each independently representing an alkyl group, aryl group, acyl group, alkenyl group or alkynyl group, and wherein a leaving group of the leuco dye is dissociated by interaction between the leuco dye and the multi-stranded nucleic acid, and a color that can be detected under visible light is then formed by dissociation of the leaving group.