Patent ID: 6342349
Filing Date: 2002-01-29
Classification: B01J,C12Q,C40B,G01N

Abstract:
An assay device comprising:a) a solid support substrate; b) a cleavable signal element having: a signal responsive moiety; and a cleavable spacer for releasably attaching said signal responsive moiety to said substrate, said cleavable spacer having an end attached to said substrate, an end attached to said signal responsive moiety and a cleavage site intermediate said substrate-attaching end and said signal responsive end; c) a first anchoring member having a first end attached to said substrate and a second end adapted to bind on a first site on a chosen analyte; d) a second anchoring member having a first end attached to said signal responsive moiety and a second end adapted to bind on a second site on said chosen analyte; and the signal responsive moiety remaining bound to the substrate after cleavage at the cleavage site only when said first and second anchoring members are bound to said chosen analyte.