Patent ID: 6803188
Filing Date: 2004-10-12
Classification: C07K,C12Q,G01N

Abstract:
A tandem fluorescent protein construct, comprising:a donor Aequorea fluorescent protein moiety, an acceptor Aequorea fluorescent protein moiety, wherein said donor and acceptor Aequorea fluorescent protein moieties have a contiguous sequence of 150 amino acids with at least 85% sequence identity with an amino acid sequence from the wild type Aequorea green fluorescent protein of SEQ ID NO:2, and a linker moiety coupling said donor Aequorea fluorescent protein moiety and said acceptor Aequorea fluorescent protein moiety, wherein said donor Aequorea fluorescent protein moiety and said acceptor Aequorea fluorescent protein moiety comprises a covalently linked fluorophore generated by the cyclization and oxidation of amino acids of said donor or acceptor Aequorea fluorescent protein, further wherein said donor Aequorea fluorescent protein moiety and said acceptor Aequorea fluorescent protein moiety exhibit fluorescence resonance energy transfer when said donor Aequorea fluorescent protein moiety is excited.