Patent ID: 6316198
Filing Date: 2001-11-13
Classification: C12N,C12Q

Abstract:
A method for detecting in a sample the presence of a nucleic acid Q' whose nucleotide sequence differs from a nucleic acid Q in at least one position A, the method comprising the steps ofa) combining nucleic acids present in the sample with an appropriate amount of nucleoside triphosphates, an agent for polymerisation of the nucleoside triphosphates and at least one diagnostic oligonucleotide containing at position A a nucleotide which is capable of hybridizing to the nucleotide at position A of nucleic acid Q' but not to a nucleotide at position A of nucleic acid Q under hybridisation conditions, the at least one diagnostic oligonucleotide containing at least one LNA,b) extending any oligonucleotides which hybridize to the nucleic acids present in the sample to form extension products, wherein said nucleic acids are used as templates,c) detecting any nucleic acids formed in step b) and thereby the presence of nucleic acid Q' in the sample.