Patent ID: 6962792
Filing Date: 2005-11-08
Classification: A61K,A61P,C07K

Abstract:
1. A method for identifying a compound which modulates binding between p21 and cyclin D1, the method including: (a) bringing into contact a first substance comprising a peptide fragment of 40 amino acids or less of p21, the peptide fragment comprising an amino acid sequence selected from the group consisting of: KACRRLFGPVDSEQLSRDCD (peptide 2) (SEQ ID NO:2); KRRQTSMTDFYHSKRRLIFS (peptide 10) (SEQ ID NO:10), wherein the amino acid D may be replaced by any amino acid; KRRQTSATDFYHSKRRLIFS (SEQ ID NO:28); TSMTDFYHSKRRLIFSKRKP (peptide 11) (SEQ ID NO:11); and KRRLIFSK (SEQ ID NO:23), wherein K, R, I or S may be replaced by any amino acid; with a second substance comprising cyclin D1 or a fragment thereof, and a test compound, under conditions wherein, in the absence of the test compound being an inhibitor of binding of said first and second substances, said first substance and said second substance bind; and (b) determining binding between said first substance and said second substance.