Patent ID: 7078208
Filing Date: 2006-07-18
Classification: C12N

Abstract:
1. A mutant M-MLV reverse transcriptase comprising a polymerase domain having RNA-dependent DNA polymerase activity and a substitution in the amino acid sequence of the wild type M-MLV polymerase domain within (SEQ ID NO 8), wherein amino acid number 1 of SEQ ID NO: 8 is the threonine following the initial methionine, corresponding to a substitution selected from the group consisting of: (a) a substitution of leucine 52 of wild type M-MLV reverse transcriptase for a different amino acid; (b) a substitution of histidine 204 of wild type M-MLV reverse transcriptase for a different amino acid; (c) a substitution of methionine 289 of wild type M-MLV reverse transcriptase for a different amino acid; and (d) a substitution of threonine 306 of wild type M-MLV reverse transcriptase for a different amino acid.