Patent ID: 6689936
Filing Date: 2004-02-10
Classification: A01K,A61K,C12N

Abstract:
A method of evaluating a compound for its effect on skin, comprising:providing a live transgenic mouse whose genome comprises a nucleic acid sequence encoding a reporter polypeptide coupled to a promoter selected from the group consisting of: a vascular endothelial growth factor (VEGF) promoter, and a versican promoter, wherein the reporter polypeptide is expressed in the skin of the mouse, and wherein the reporter polypeptide is a luminescent or fluorescent product; administering the compound to the transgenic mouse; and evaluating expression of the nucleic acid sequence encoding the reporter polypeptide in the skin of the live transgenic mouse while the skin is still on the mouse, wherein an alteration in expression of the nucleic acid sequence encoding the reporter polypeptide after administering the compound as compared to expression of the nucleic acid encoding the reporter polypeptide prior to administering the compound is indicative of an effect on skin, thereby evaluating the compound for its effect on skin.