Patent ID: 8569036
Filing Date: 2013-10-29
Classification: C12N,C12P,Y02P

Abstract:
1. An immobilized tyrosine ammonia lyase biocatalyst comprising: a) a bacterial cell tolerant to para-hydroxycinnamic acid and alkaline pH and transformed with a genetic construct comprising a gene, the gene encoding a tyrosine ammonia lyase (TAL) enzyme selected from the group of TAL enzymes having TAL enzyme activity from b) a cross-linked alginate bead prepared by a process in which glutaraldehyde is used at a final concentration of between about 2.5×10 wherein the bacterial cell is embedded in the alginate bead; and wherein the tyrosine ammonia lyase enzyme has an activity about 1.9 to about 8.0 measured as g pHCA produced/L/h per g dcw bead catalyst.