Patent ID: 8071317
Filing Date: 2011-12-06
Classification: G01N,Y10S,Y10T

Abstract:
1. A method for determining a hydrophobic immunosuppressant drug in a sample suspected of containing a hydrophobic immunosuppressant drug, the method comprising: (a) providing in combination in a medium: (b) incubating the medium under conditions for enhancing the bioavailability of the hydrophobic immunosuppressant drug over that of the metabolites in the medium, (c) adding to the medium of step b), (i) an enzyme labeled antibody that is specific for and binds to the immunosuppressant drug and (ii) magnetic particles having bound thereto an analog of the hydrophobic immunosuppressant drug analog thereof, and (d) examining the medium and detecting for the presence of the label, the presence thereof indicating the presence of a complex comprising the hydrophobic immunosuppressant drug and the antibody specific for the immunosuppressant drug, the presence and/or amount of the complex indicating the presence and/or amount of the hydrophobic immunosuppressant drug in the sample.