Patent ID: 8927217
Filing Date: 2015-01-06
Classification: C12Q

Abstract:
1. A method for determining the absence of microcystin-producing cyanobacteria and nodularin-producing cyanobacteria in a sample, the method comprising analyzing a cyanobacterial sample for the absence of a sequence of a hepatotoxin-associated aminotransferase domain, wherein: said sequence is common to the mcyE open reading frame of a microcystin synthetase gene complex and the ndaF open reading frame of a nodularin synthetase gene complex; said sequence is located between the polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) modules within the mcyE and ndaF open reading frames; and said absence of said sequence indicates an absence of microcystin-producing cyanobacteria and nodularin-producing cyanobacteria in said sample; and wherein the method comprises using a single primer pair capable of amplifying at least a portion of the hepatotoxin-associated aminotransferase domain from microcystin-producing cyanobacteria and nodularin-producing cyanobacteria.