Patent ID: 6607892
Filing Date: 2003-08-19
Classification: A61P,C12N

Abstract:
A method for screening test compounds to identify a compound that binds to a polypeptide comprising an amino acid sequence selected from the group consisting of:a) the amino acid sequence of a sequence variant of the amino acid sequence set forth in SEQ ID NO:1, wherein said sequence variant has adenylate cyclase activity and is encoded by a nucleotide sequence having at least 75% overall sequence identity with the nucleotide sequence shown in SEQ ID NO:2; b) the amino acid sequence of a sequence variant of the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Patent Deposit No. PTA-1661, wherein said sequence variant has adenylate cyclase activity and is encoded by a nucleotide sequence having at least 75% overall sequence identity with the nucleotide sequence of the cDNA insert of the plasmid deposited with the ATCC as Patent Deposit No. PTA-1661; c) the amino acid sequence of a fragment of the amino acid sequence set forth in SEQ ID NO:1, wherein said fragment has adenylate cyclase activity and consists of at least 100 contiguous amino acids of the amino acid sequence set forth in SEQ ID NO:1; and d) the amino acid sequence of a fragment of the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Patent Deposit No. PTA-1661, wherein said fragment has adenylate cyclase activity and consists of at least 100 contiguous amino acids of the amino acid sequence encoded by the cDNA insert of the plasmid deposited with the ATCC as Patent Deposit No. PTA-1661; said method comprising contacting the test compound with the polypeptide or a cell expressing the polypeptide under conditions suitable for binding of the compound to the polypeptide and determining whether a complex is formed between the test compound and the polypeptide.