Patent ID: 7790379
Filing Date: 2010-09-07
Classification: C12Q

Abstract:
1. A method for localizing within a chromosome or a DNA, the DNA binding loci of a chromatin binding protein or a DNA binding protein comprising the following steps: a) linking, directly or indirectly, to said chromatin binding protein or said DNA binding protein, an enzyme with regulatable activity, wherein the enzyme is capable of introducing into said chromosome or said DNA a detectable modification; and is in an inactive state when so linked; b) contacting the chromosome or the DNA with the chromatin binding protein or the DNA binding protein to which the enzyme is linked from step a); c) transiently activating the regulatable activity of the enzyme bound to the chromatin binding protein or the DNA binding protein so that the enzyme introduces the detectable modification into the chromosome or the DNA; and d) mapping on the chromosome or the DNA the location of the enzymatic modification introduced by the enzyme into said chromosome or said DNA so as to thereby localize within the chromosome or the DNA, the DNA binding loci of the chromatin binding protein or the DNA binding protein.