Patent ID: 8445254
Filing Date: 2013-05-21
Classification: A61K,A61P,C12N

Abstract:
1. A recombinant bacterium capable of the regulated expression of at least one nucleic acid sequence encoding an antigen of interest, and capable of regulated attenuation, wherein the bacterium comprises a. at least one chromosomally integrated nucleic acid sequence encoding a repressor operably linked to the regulatable promoter P b. a vector comprising a nucleic acid sequence encoding at least one antigen of interest operably linked to a promoter regulated by the repressor, such that the expression of the nucleic acid sequence encoding the antigen is repressed during in vitro growth of the bacterium, but the bacterium is capable of high level expression of the nucleic acid sequence encoding the antigen in a host, c. a regulatable promoter chromosomally integrated so as to replace the native promoter of, and be operably linked to, at least one nucleic acid sequence of an attenuation protein, and d. the mutation ΔP