Patent ID: 6448018
Filing Date: 2002-09-10
Classification: G01N

Abstract:
A fluorescence polarization method at multiple wavelengths for analyzing two or more different assay-objects in a sample, the method comprising the steps of:(a) providing two or more different fluorescent-labeled substances, each being a substance which is capable of specifically binding to respective one of the assay-objects and is covalently bound to a fluorochrome, wherein the fluorochromes of the fluorescent-labeled substances are different from one another, wherein the fluorescence lifetime of each fluorochrome is selected based on the change in molecular weight of the fluorescent-labeled substance resulting from the binding to the assay-object, and wherein each of the fluorochromes is selected so that the fluorochrome is different from the other fluorochromes in terms of excitation wavelength and/or fluorescence wavelength; (b) allowing the fluorescent-labeled substances to bind to the two or more different assay-objects respectively; and (c) measuring a change in the degree of fluorescence polarization which has taken place in each of the fluorescent-labeled substances by its binding to one of the assay-objects.