Patent ID: 9062392
Filing Date: 2015-06-23
Classification: B82Y,C07K,C12N,C40B

Abstract:
1. A method for identifying a peptide comprising: incubating a first peptide library with a first set of nucleic acid oligonucleotides to identify a first sub-library of peptides that bind the first set of nucleic acid oligonucleotides; separating the first sub-library from the first library; contacting the first sub-library with a second set of nucleic acid oligonucleotides comprising homo-oligomers of nucleotides other than a preselected nucleotide, to identify a second sub-library of peptides that do not bind the second set of nucleic acid oligonucleotides; separating the second sub-library from the first sub-library; contacting the second sub-library with a third subset of nucleic acid oligonucleotides comprising a homogeneous population of homo-oligonucleotides comprising the pre-selected nucleotide and no other nucleotides, to identify a third sub-library of peptides that bind to the third subset of nucleic acid oligonucleotides; separating the third sub-library from the second sub-library; contacting the third sub-library with a plurality of a first set of aminolated nucleotides comprising a heterogeneous population of nucleotides other than the pre-selected nucleotide, to identify a fourth sub-library of peptides that do not bind the first set of aminolated nucleotides; separating the fourth sub-library from the fifth sub-library; and contacting the fifth sub-library with a second set of aminolated nucleotides, comprising a homogeneous population of the pre-selected nucleotide, to produce a sixth sub-library of peptides that bind the second set of aminolated nucleotides, thereby identifying a peptide that binds the pre-selected nucleotide.