Patent ID: 6455256
Filing Date: 2002-09-24
Classification: C12Q

Abstract:
A method of amplifying nucleic acids from a target microorganism present in fruit juice comprising the steps of:a) carrying out a microorganism concentration step comprising passing said fruit juice through a dissolvable filter having a pore size selected to retain said target microorganisms; b) immediately following said concentration step with the step of extracting the retentate from the filter with an organic solvent selected to dissolve said filter while not interfering with the integrity of nucleic acids present in the cells of said microorganisms; c) subjecting the extracted retentate to an organic wash step; d) subjecting the extracted retentate to an aqueous wash step; e) concentrating said retentate; and f) subjecting nucleic acids present in said concentrated retentate to an amplification procedure or a detection procedure.