Patent ID: 6541644
Filing Date: 2003-04-01
Classification: B01D,C07D

Abstract:
A process for isolating substantially pure L-&bgr;-3-indolylalanine (L-&bgr;-3) from a source of amino acids that includes L-&bgr;-3, L-phenylalanine, L-tyrosine and aliphatic amino acids; the process comprising:providing an aqueous solution of amino acids; contacting the aqueous solution with a porous wettable polymeric resin to allow a substantial portion of L-&bgr;-3, L-phenylalanine and L-tyrosine to adsorb to the resin and to provide a first solution containing aliphatic amino acids, wherein the resin is attractive to aromatic amino acids and has substantially no attraction to aliphatic amino acids; eluting the resin with water to displace any residual aliphatic amino acids from the resin to provide a second solution containing aliphatic amino acids; eluting the resin with a dilute acid to displace L-phenylalanine and L-tyrosine from the resin and provide a third solution containing L-phenylalanine and L-tyrosine; eluting the resin with a dilute base to displace L-&bgr;-3 from the resin and provide a fourth solution containing L-&bgr;-3 displaced from the resin; and recovering L-&bgr;-3 from the fourth solution.