Patent ID: 7094542
Filing Date: 2006-08-22
Classification: C12Q

Abstract:
1. A method of detecting rpoB sequences of Mycobacterium tuberculosis present in a biological sample, comprising the steps of: providing a biological sample containing nucleic acid from amplifying the rpoB sequence in an in vitro nucleic acid amplification reaction mixture comprising at least one polymerase activity, and primers consisting of SEQ ID NO:2 with SEQ ID NO: 3, to produce amplified nucleic acid containing a rpoB sequence; optionally fragmenting the amplified nucleic acid; hybridizing the amplified nucleic acid to at least one detection probe consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 or SEQ ID NO:4 that hybridizes specifically to detecting the amplified nucleic acid hybridized to at least one detection probe by detecting a label associated with the amplified nucleic acid.