Patent ID: 6455312
Filing Date: 2002-09-24
Classification: A01H,C12N

Abstract:
A method of producing a perennial grape embryogenic culture, said method comprising the steps of:(a) visually identifying an embryogenic cell or an embryogenic cell mass from a first grape explant culture, wherein said visually identified embryogenic cell or cells of said embryogenic cell mass are identified as having a white to pale yellow color; (b) transferring said visually identified embryogenic cell or embryogenic cell mass of step (a) to a second culture; (c) allowing said transferred embryogenic cell or embryogenic cell mass of step (b) to divide into additional embryogenic cells or embryogenic cell masses; (d) visually identifying an embryogenic cell or embryogenic cell mass of step (c), wherein said visually identified embryogenic cell or cells of said embryogenic cell mass of step (c) are identified as having a white to pale yellow color; and (e) transferring said visually identified embryogenic cell or embryogenic cell mass of step (d) to a third culture thereby producing a perennial grape embryogenic culture.