Patent ID: 9193994
Filing Date: 2015-11-24
Classification: C12Q

Abstract:
1. A composition for amplifying a target nucleic acid, the composition comprising: (a) a target nucleic acid; (b) a first polynucleotide comprising at least two complementary regions that are complementary to the target nucleic acid, wherein a first complementary region comprises at least one consecutive nucleotide from the 3′-terminal thereof that is complementary to the 3′-terminal region of the target nucleic acid, wherein at least one second complementary region comprises at least one consecutive nucleotide 5′ of the first complementary region in the first polynucleotide that is complementary to the 5′ terminal region of the target nucleic acid; and wherein the first complementary region and the at least one second complementary region are separated by a region of the first polynucleotide that is non-complementary to the target nucleic acid; (c) a second polynucleotide consisting of a third complementary region that is complementary to the first polynucleotide in the region that is non-complementary to the target nucleic acid, wherein the 3′ terminus of the second polynucleotide is modified.