Patent ID: 8945898
Filing Date: 2015-02-03
Classification: C12N,C12P

Abstract:
1. A method for production of a compound of interest by microbial fermentation comprising: a. providing a microbial host cell, b. culturing said microbial host cell in a culture medium under conditions conducive to expression of the compound of interest, and c. isolating the compound of interest from the culture medium, wherein said microbial host cell has been modified in the genome thereof resulting in a deficiency in production of at least one non-ribosomal peptide synthase, wherein said microbial host cell is a filamentous fungus, and wherein said at least one non-ribosomal peptide synthase is at least 95% identical to a polypeptide selected from the group consisting of the polypeptide according to SEQ ID NO: 38 (npsE), and the polypeptide according to SEQ ID NO: 34 (Pc16g04690), and/or wherein said microbial host cell has been modified in its genome such that it results in a reduction of the amount of at least one mRNA having at least 90% identity with a mRNA selected from the group consisting of the mRNA according to SEQ ID NO: 37, and the mRNA according to SEQ ID NO: 33.