Patent ID: 8993350
Filing Date: 2015-03-31
Classification: G01N,Y10T

Abstract:
1. A method to detect free polyethylene glycol-maleimide (PEG-mal) in a solution comprising free PEG-mal and PEGylated protein said method comprising the steps of (a) adding the fluorescent labeled peptide probe consisting of SEQ ID NO: 1, to said solution; (b) allowing the fluorescent labeled peptide probe in the solution of step (a) to react under dark conditions with the free PEG-mal present in the solution to provide fluorescent labeled PEG-mal; (c) separating by fractionation the fluorescent labeled PEG-mal from the PEGylated protein using high performance liquid chromatography (HPLC) wherein the fluorescent fraction identifies the free PEG-mal.