Patent ID: 8617819
Filing Date: 2013-12-31
Classification: C12Q,G01N

Abstract:
1. A method of determining a biological entity, comprising: exposing a sample suspected of containing a biological entity to at least two fluorescent pi-conjugated polymers, each polymer comprising a phenylene ethynylene backbone having at least two phenylene ethynylene groups and a plurality of biological recognition elements covalently attached to the pi-conjugated polymer via the phenyl groups of the phenylene ethynylene backbone, wherein the biological recognition elements are able to specifically interact with the biological entity; allowing the biological entity, if present, to interact with the biological recognition elements of the at least two fluorescent pi-conjugated polymers in a manner bringing said at least two fluorescent pi-conjugated polymer into proximity with each other such that an emissive signal is produced at a threshold level; and determining fluorescence of the sample.