Patent ID: 8187836
Filing Date: 2012-05-29
Classification: C07K,C12P

Abstract:
1. An expression vector comprising: (a) an OriP origin of replication derived from Epstein-Barr virus (EBV); (b) an SV40 origin of replication; (c) an insertion site for inserting a gene of interest; (d) a promoter operably linked to the insertion site, wherein the promoter is an EF-1α promoter comprising nucleotides 76 to 1267 of SEQ ID NO: 2; and, optionally, (e) a nucleic acid sequence encoding an antibody heavy or light chain constant region, operably linked to the insertion site, wherein the OriP origin of replication is bound by a trans-acting EBNA1 replication initiation factor that is not encoded by the expression vector.