Patent ID: 9217157
Filing Date: 2015-12-22
Classification: A61P,C07K,C12N

Abstract:
1. A pathogenic recombinant influenza virus comprising a nucleic acid sequence comprising a modified influenza virus nonstructural (NS) gene segment or the complement thereof, wherein the modified influenza virus NS gene segment comprises in 3′ to 5′ order: (a) (i) an influenza virus nonstructural protein 1 (NS1) open reading frame (ORF) lacking a stop codon, (ii) a heterologous nucleotide sequence encoding a detectable substance, (iii) a cleavage site, and (iv) an influenza virus nuclear export protein (NEP) ORF, wherein the modified influenza virus NS gene segment has one or more mutations in either the splice acceptor site, the splice donor site, or both the splice acceptor and splice donor sites that prevents splicing of mRNA, and wherein the modified influenza virus NS gene segment encodes (1) a fusion protein comprising NS1 and the detectable substance and (2) a protein comprising NEP; or (b) (i) an influenza virus NS1 ORF lacking a stop codon, (ii) a linker, (iii) a heterologous nucleotide sequence encoding a detectable substance, (iv) a cleavage site, and (v) an influenza virus NEP ORF, wherein the modified influenza virus NS gene segment has one or more mutations in either the splice acceptor site, the splice donor site, or both the splice acceptor and splice donor sites that prevents splicing of mRNA, and wherein the modified influenza virus NS gene segment encodes (1) a fusion protein comprising NS1, the amino acids encoded by the linker, and the detectable substance, and (2) a protein comprising NEP.