Patent ID: 8175369
Filing Date: 2012-05-08
Classification: G06K

Abstract:
1. A computer-implemented method for counting nuclei of a plurality of cells in a cell-containing sample, the method comprising: receiving a raw image of the cell-containing sample; transforming the raw image into a segmented image comprising one or more nuclei clusters on a contrasting background; for each of the one or more nuclei clusters: obtaining a convex hull of the nuclei cluster; locating any indentations on the nuclei cluster by comparing the nuclei cluster to the convex hull of the nuclei cluster; calculating a first nuclei count for the nuclei cluster based on a tally of the indentations; and assigning the nuclei cluster to a cell among the plurality of cells; calculating a second nuclei count for each cell of the plurality of cells by totaling the first nuclei counts of its constituent nuclei clusters; and generating a result based on the second nuclei count for at least one of the plurality of cells.