Patent ID: 6320103
Filing Date: 2001-11-20
Classification: C12N

Abstract:
A transgenic plant having enhanced osmotolerance comprising a nucleic acid construct comprising a nucleic acid sequence encoding a polypeptide having choline oxidase activity obtained from Fusarium venenatum, selected from the group consisting of:(a) a nucleic acid sequence encoding a polypeptide having an amino acid sequence which has at least 95% identity with amino acids 1 to 543 of SEQ ID NO:2;(b) a nucleic acid sequence having a sequence which has at least 95% homology with nucleotides 49 to 1677 of SEQ ID NO:1; and(c) a nucleic acid sequence, encoding a polypeptide having choline oxidase activity, which hybridizes under low stringency conditions with (i) nucleotides 49 to 1677 of SEQ ID NO:1, (ii) a sequence of (i) of at least 100 contiguous nucleotides, or (iii) a complementary strand of (i) or (ii), wherein low stringency conditions are defined as prehybridization and hybridization at 42.degree. C. in 5.times.SSPE, 0.3% SDS, 200 .mu.g/ml sheared and denatured salmon sperm DNA, and 25% formamide, and washing with 2.times.SSC, 0.2% SDS at 50.degree. C.