Patent ID: 7709256
Filing Date: 2010-05-04
Classification: C12M,C12N,G01N

Abstract:
1. An in vitro three culture cellular system, comprising: (i) a vaccination site (VS) culture comprising a first substantially planar matrix, a plurality of cells consisting of endothelial cells and/or epithelial cells attached to said first matrix and a test agent, for receiving a population of peripheral blood mononuclear cells (PBMCs) and for maturing dendritic cell precursors present in said population of PBMCs; (ii) a three-dimensional artificial lymphoid tissue equivalent (LTE) culture comprising a second matrix and a plurality of lymphocytes attached to said second matrix, for receiving dendritic cells matured in said VS culture; and (iii) a diseased cell culture for receiving immunocytes and/or soluble factors from the LTE culture.