Patent ID: 6162645
Filing Date: 2000-12-19
Classification: G01N,Y10S

Abstract:
A one-read method of determining the percentage of glycated hemoglobin (GHb) in a whole blood sample without having to measure total hemoglobin (Hb), which comprises the steps of:a) lysing a whole blood sample to release glycated hemoglobin;b) incubating said lysed blood sample with a solid phase, said solid phase being coupled to a boronate moiety, wherein said solid phase specifically captures glycated hemoglobin (Hb) in direct proportion to the percentage of glycated hemoglobin (Hb) in the sample;c) adding a labeled component specific for hemoglobin to said sample;d) measuring a resultant signal; ande) determining a percentage of glycated hemoglobin in the sample based on said resultant signal.