Patent ID: 8597912
Filing Date: 2013-12-03
Classification: C07K,C12N

Abstract:
1. A protein comprising an engineered zinc finger protein DNA-binding domain that specifically binds to a target site in a Bak or Bax gene, wherein the DNA-binding domain comprises four, five or six zinc finger recognition regions, ordered F1 to F4 for proteins with four zinc finger domains, ordered F1 to F5 for proteins with five zinc finger domains and ordered F1 to F6 for proteins with six zinc finger domains from N-terminus to C-terminus, and further wherein the zinc finger proteins is selected from the group consisting of a protein comprising the following amino acid sequences in the zinc finger recognition regions: (i) F1: RSDHLTT (SEQ ID NO:9); (ii) F1: RSDSLSA (SEQ ID NO:14); (iii) F1: DRSHLSR (SEQ ID NO:34); (iv) F1: RSDNLSV (SEQ ID NO:39); (v) F1: QNAHRKT (SEQ ID NO:50); (vi) F1: RSDNLAR (SEQ ID NO:57); (vii) F1: RSDNLTT (SEQ ID NO:62); (viii) F1: QSGSLTR (SEQ ID NO:67); (ix) F1: RSDHLST (SEQ ID NO:1); (x) F1: RSANLSV (SEQ ID NO:5); (xi) F1: DRSDLSR (SEQ ID NO:45); (xii) F1: RSDALSV (SEQ ID NO:48);