Patent ID: 8859238
Filing Date: 2014-10-14
Classification: C12Q

Abstract:
1. A method comprising the steps of: a. Providing a template nucleic acid b. Providing a bifunctional oligonucleotide (bfo) comprising a primer/template (pt) region (complementary to part of the template nucleic acid) in its 3′end and a capture region (cr), wherein the pt region and the cr region is separated by a polymerase block (B) c. Mixing the components of steps a-b and providing conditions that allow the pt region of the bfo to anneal to the template nucleic acid and the B to improve the production of amplified template nucleic acid d. Under conditions allowing primer extension, extending the pt region of the bfo e. Thereby generating a double stranded nucleic acid with a single stranded overhang (dso) corresponding to the cr region of the bfo wherein R is selected from the group consisting of arylethynyl, pyreneethynyl, and 1H-phenanthro[9,10-d]imidazol-2-yl.