Patent ID: 7928199
Filing Date: 2011-04-19
Classification: A61K,A61P,C07K

Abstract:
1. A method of stabilizing a Factor VIII polypeptide which is the product of a single gene in nature by introducing one or more cysteines comprising the steps of: (a) obtaining or creating a three-dimensional structure of said polypeptide; (b) predicting one or more sites for the introduction of one or more cysteines based on said structure; and (c) creating one or more mutants of said polypeptide by introducing one or more cysteines at one or more of said predicted sites; wherein at least one pair of cysteines is located at residues: Met 662-Asp 1828; Ser 268-Phe 673; Ile 312-Pro 672; Ser 313-Ala 644; Met 662-Lys 1827; Tyr 664-Thr 1826; Pro 264-Gln 645; Arg 282-Thr 522; Ser 285-Phe 673; His 311-Phe 673; Ser 314-Ala 644; Ser 314-Gln 645; Val 663-Glu 1829; Asn 694-Pro 1980; or Ser 695-Glu 1844 of the Factor VIII polypeptide.