Patent ID: 6110667
Filing Date: 2000-08-29
Classification: C12Q

Abstract:
A process for characterizing one or more nucleotide sequences in a sample, each such sequence comprising one or more nucleotides, wherein each sequence is characterized by said process comprising the steps ofA) contacting each nucleotide sequence in said sample, or respective portions thereof, with a plurality of primer pairs, each pair consisting of:(i) a statistically selected K-tuple 5'-primer for hybridizing with relative high frequency to a respective nucleotide subsequence, a complementary subsequence thereto, putatively present in said sample, and(ii) a statistically selected K-tuple 3'-primer for hybridizing with relative high frequency to a different respective nucleotide subsequence, or complementary subsequence thereto, putatively present in said sample, andB) detecting regions of said nucleotide sequences in said sample which are delineated by subsequences hybridized with each primer pair,C) determining a physical characteristic of each such detected region, andD) indexing the physical characteristic of at least one such detected region by an identity of each primer pair that hybridized to its delineating subsequences.