Patent ID: 6172209
Filing Date: 2001-01-09
Classification: A61P,C07H,Y02P

Abstract:
An oligonucleotide specifically hybridizable with DNA or RNA comprising a sequence of from about 5 to about 50 nucleotide units, wherein:said sequence is divided into a first region having 2'-O-alkyl substituted nucleotide units and a second region being composed of nucleotide units having 2'-deoxy sugar moieties;said nucleotide units of at least one of said first or second regions are connected by phosphorothioate linkages; andat least one of said 2'-O-alkyl substituted nucleotide units of said first region bears a 2'-aminooxy group having one of the formulas: ##STR4##wherein:m is from 1 to 10;y is from 1 to 10;x is 1;E is N (R.sub.1)(R.sub.2), --NH--CH(R.sub.1)(R.sub.2) or N.dbd.C(R.sub.1)(R.sub.2); andeach R.sub.1 and R.sub.2 is, independently, H, C.sub.1 -C.sub.10 alkyl, a nitrogen protecting group, or R.sub.1 and R.sub.2, together, are a nitrogen protecting group or wherein R.sub.1 and R.sub.2 are joined in a ring structure that can include at least one heteroatom selected from N and O.