Patent ID: 6027722
Filing Date: 2000-02-22
Classification: A61K,C12N

Abstract:
A vector for transferring and expressing nucleic acid sequences comprising vector sequences, the vector sequences comprising linked:(a) a 5' long terminal repeat (LTR) sequence from a VL30 retrotransposon comprising a transcription initiation site for RNA;(b) an encapsidation sequence positioned 3' of the 5' LTR;(c) a primer binding site sequence from a VL30 retrotransposon positioned 3' of the 5' LTR;(d) a 3' LTR sequence from a VL30 retrotransposon positioned 3' of the primer binding site, wherein the 3' LTR includes:(1) sequences which cause polyadenylation of a RNA transcript initiated in the 5' LTR;(2) sequences which provide a primer for reverse transcription of the RNA transcript from step (d)(1) into a double stranded cDNA;(e) a polypurine tract sequence from a VL30 retrotransposon located 5' to the 3'LTR; and(f) sequences within each LTR which are cleaved prior to integration of the vector into the genome of a recipient cell,wherein the vector sequences comprise no more than 2 kbp.