Patent ID: 9175281
Filing Date: 2015-11-03
Classification: C12N,C12Y

Abstract:
1. A process for the purification of a tissue plasminogen activator protein comprising: a) loading a mixture of a tissue plasminogen activator protein with impurities on to a suitable column matrix; b) eluting the impurities from the column matrix with a suitable organic solvent at appropriate concentration; c) eluting the desired form of the tissue plasminogen activator protein from the column matrix with a suitable organic solvent at appropriate concentration; d) obtaining the tissue plasminogen activator protein in its desired form; and e) optionally, an ion exchange column step is used before or after using the suitable column matrix; wherein the suitable organic solvent is ethanol, methanol, or isopropyl alcohol, and wherein the suitable column matrix is hydrophobic interaction chromatography.