Patent ID: 6617138
Filing Date: 2003-09-09
Classification: C12Q

Abstract:
A method of detecting a target nucleotide sequence in a nucleic acid molecule which comprises:(a) binding of an oligonucleotide probe to said nucleic acid molecule to provide a complex between the oligonucleotide probe and nucleic acid molecule; (b) selective labeling of the bound oligonucleotide probe in the presence of said target nucleotide sequence; (c) denaturation of the complex between the oligonucleotide probe and nucleic acid molecule; (d) incubation of the labeled and any unlabeled oligonucleotide and a complementary sequence under conditions whereby labeled oligonucleotide hybridizes to the complementary sequence; and (e) subsequent detection of the label wherein detection of the label is indicative of detection of the target nucleotide sequence.