Patent ID: 8486422
Filing Date: 2013-07-16
Classification: C07K,C12P

Abstract:
1. A method of activating a modified Clostridial toxin, the method comprising the step of incubating a modified Clostridial toxin with a BoNT/A di-chain loop protease under physiological conditions; wherein the BoNT/A toxin di-chain loop protease is SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 35, SEQ ID NO: 36, SEQ ID NO: 37 or SEQ ID NO: 38; and wherein cleavage of the modified Clostridial toxin by the BoNT/A di-chain loop protease converts the modified Clostridial toxin from its single-chain polypeptide form into its di-chain form, thereby activating the modified Clostridial toxin.