Patent ID: 6525245
Filing Date: 2003-02-25
Classification: C07K,C12N,C12Q

Abstract:
A method for identifying the nucleic acid sequence of a gene encoding a protein that participates in a signal transduction pathway between mitochondrial function and nuclear gene expression in Arabidopsis thaliana comprising:(a) transforming the Arabidopsis thaliana with a vector, the vector comprising a gene that encodes a reporter protein operably linked to an Arabidopsis thaliana AOX1a promoter consisting essentially of a nucleic acid sequence of SEQ ID NO:4 or the complement thereof; (b) identifying a transgenic Arabidopsis thaliana from step a that increases the expression of the gene encoding the reporter protein when subjected to a stimulus relative to the basal level of endogenous expression of the gene; (c) mutating the transgenic Arabidopsis thaliana identified in step b; (d) selecting a mutant transgenic Arabidopsis thaliana from step c, wherein the mutant Arabidopsis thaliana exhibits altered expression of the gene encoding the reporter protein relative to an unmutagenized Arabidopsis thaliana; (e) determining the nucleic acid sequence of the gene that encodes the protein that participates in the signal transduction pathway in the mutant Arabidopsis thaliana from step d.