Patent ID: 6379929
Filing Date: 2002-04-30
Classification: B01J,B01L,B82Y,C12Q

Abstract:
A method for isothermal amplification of at least one selected nucleic acid, comprising:a) providing at least one sample comprising at least one selected nucleic acid, and reagents effective to permit at least one isothermal amplification reaction, to at least one microfabricated substrate that defines at least one channel, said channel connected to at least one isothermally regulated reaction chamber, wherein said sample is conveyed from said channel to said isothermally regulated reaction chamber by differential heating of said sample, wherein said substrate further comprises at least a second channel or at least one reservoir, wherein said at least a second channel or at least one reservoir is directly or indirectly connected to said at least one reaction chamber, wherein said reagents are prefabricated in a lyophilized form into said at least one reaction chamber, said at least one channel, said at least a second channel or said at least one reservoir; and b) conducting at least one isothermal amplification reaction to amplify said selected nucleic acid.