Patent ID: 7112414
Filing Date: 2006-09-26
Classification: A61K,A61P,C07K,C12N,C12Y,G01N

Abstract:
1. A method of assaying for the immune defense complement pathway activating activity of a mature MASP-2 polypeptide in a complex with mannan-binding lectin (MEL) wherein the mature MASP-2 polypeptide comprises an amino acid sequence having at least 95% amino acid sequence identity with the amino acid sequence set forth in SEQ ID NO:2 from position 16 through position 686, the method comprising: (i) obtaining a sample comprising MBL/MASP-2 complexes, (ii) contacting said sample with at least one MASP-2 substrate in a solution having a salt concentration greater than 0.2M, and, (iii) detecting the amount of MASP-2 substrate cleaved, whereby the MBL/MASP-2 complex complement pathway activating activity is quantified.