Patent ID: 9206432
Filing Date: 2015-12-08
Classification: C07K,C12N

Abstract:
1. A method of regulating transcription of a polynucleotide of interest in a host cell comprising: (a) providing the host cell comprising the polynucleotide of interest, wherein the polynucleotide of interest is operably linked to a promoter comprising at least one operator sequence; (b) providing a recombinant sulfonylurea-responsive repressor polypeptide that specifically binds to the operator sequence; and, (c) providing a sulfonylurea compound, wherein the sulfonylurea binds to the polypeptide to form a complex that modifies the binding properties of the polypeptide to the operator, wherein the sulfonylurea-responsive polypeptide comprises an amino acid sequence having at least 95% sequence identity when compared to the full-length sequence set forth in SEQ ID NO:1233.