Patent ID: 7288372
Filing Date: 2007-10-30
Classification: C07H,G01N,Y02P

Abstract:
1. A method, comprising: a) providing: b) attaching said protective group to said alpha amino group of said markerless amino acid to form a protected markerless amino acid; c) converting said protected markerless amino acid so as to form a protected amino acid ester; and d) reacting said protected markerless amino acid ester with said unprotected polynucleotide under conditions such that a markerless aminoacyl-polynucleotide conjugate comprising said protective group is formed; and e) ligating said conjugate of step d) with a tRNA molecule lacking y nucleotides at the 3′ terminus, wherein y is an integer between 0 and 10, so as to form a markerless tRNA conjugate comprising said protective group attached to said alpha amino group.