Patent ID: 9194801
Filing Date: 2015-11-24
Classification: B01J,G01K,G01N

Abstract:
1. A method for measuring temperature through a transition of one or more strands of DNA from a coupled configuration to a decoupled configuration, the method comprising: receiving a solution in a receptacle, the solution including the DNA as a suspension in phosphate buffered saline and a fluorescent dye adapted emit fluorescence when the DNA is in the coupled configuration; placing the receptacle in proximity to a surface, thereby permitting the transition of the DNA from the coupled configuration to the decoupled configuration if the temperature of the surface reaches a temperature threshold; acquiring a sequence of images of fluorescence emitted from the solution over a time period, each image having a plurality of regions; determining a plurality of fluorescence levels corresponding to each of the plurality of regions of each image; generating a temperature map based on the determined fluorescence levels for each image over the time period; and obtaining a thermal profile of the surface over the time period from the generated temperature maps for each image.