Patent ID: 6770435
Filing Date: 2004-08-03
Classification: C07K,C12Q

Abstract:
A method of detecting the presence of a nucleotide sequence comprising SEQUENCE ID NO 6, in a test breast tissue sample, said method comprising:(a) contacting the test sample with a nucleic acid probe comprising a nucleotide sequence fully complementary to SEQUENCE ID NO 6, under hybridization conditions of 5Ã—SSC, 50% formamide, 5Ã—Denhardt's solution, 100 &mgr;g/ml denatured salmon sperm DNA for hybridization to occur between said nucleotide sequence comprising SEQUENCE ID NO 6 and said nucleic acid probe; and (b) detecting the presence of hybrids resulting from step (a), presence of said hybrids indicating presence of said nucleotide sequence comprising SEQUENCE ID NO 6 in said test sample.