Patent ID: 8877448
Filing Date: 2014-11-04
Classification: A61K,C07K,C12N

Abstract:
1. A method for rescuing a chimeric recombinant influenza virus, wherein said chimeric recombinant influenza virus expresses heterologous nucleic acid sequences, comprising: (a) introducing expression vectors into cultured cells in the absence of helper virus, wherein said expression vectors direct expression of: so that ribonucleoprotein complexes are formed and viral particles of said virus are assembled in the absence of helper virus, wherein said heterologous nucleic acid sequences are inserted into a DNA complement of a influenza virus gene, wherein the DNA complement comprises a viral polymerase binding site, and a polyadenylation site, wherein said heterologous nucleic acid sequences are flanked by a viral polymerase binding site, and a polyadenylation site; and (b) culturing the cells of step (a), wherein viral particles are packaged and rescued.