Patent ID: 8560243
Filing Date: 2013-10-15
Classification: C12Q,G01N,G16B,G16C

Abstract:
1. A method of determining a therapeutic index of a drug in a biological sample comprising: applying a plurality of levels of said drug to said biological sample; measuring transcripts of a plurality of genes at different levels of said plurality of levels of said drug in said biological sample to obtain a first profile of graded drug response; applying said plurality of levels of said drug to a test sample, wherein said test sample is the same as said biological sample except that a target pathway is not functional; measuring transcripts of said plurality of genes in said test sample at different levels of said plurality of levels of said drug, to obtain a second profile of graded drug response; based at least on said first profile of graded drug response, determining C based at least on said second profile of graded drug response, determining C determining said therapeutic index (TI) according to a formula: wherein the therapeutic index is relied upon as measurement of activity of the drug in the target pathway and the off-target pathway.