Patent ID: 8753814
Filing Date: 2014-06-17
Classification: C09B,C12Q,G01N,Y10S,Y10T

Abstract:
1. A method of staining a sample comprising a nucleic acid embedded in a matrix or immobilized on a surface comprising: exposing the sample to an aqueous solution comprising a fluorescent nucleic acid dye having the formula: wherein: Q Q Q (i) each of Q R (ii) each of Q wherein R when R when R X of Formula II is selected from O and S; n of Formula II is selected from 0, 1, and 2; R R R each of R R only one of R Ψ of Formula II is an anion; or (iii) each of Q wherein each R one of R when R when R each R each R for each pair of adjacent R Ψ of Formula III is an anion; and BRIDGE is an aliphatic linker comprising from 8 to 150 non-hydrogen atoms, inclusive, and the linker comprises no more than one positive charge.