Patent ID: 6210978
Filing Date: 2001-04-03
Classification: G01N,Y10S

Abstract:
A method for the immobilization of deoxypyridinoline onto a solid support of nitrocellulose which method comprises contacting the solid support which has a region on its surface which bears a binding reagent of anti fluorescein antibody immobilized thereon with a solution of a conjugate of fluorescein/amino acid derivatized poly (ethylene glycol)/deoxypyridinoline so that the anti fluorescein antibody binding reagent and the fluorescein/amino acid derivatized poly (ethylene glycol)/deoxypyridinoline conjugate interact with the solid nitrocellulose support bearing the anti-fluorescein antibody by a specific reaction between the anti-fluorescein antibody binding reagent and the fluorescein/amino acid derivatized poly (ethylene glycol)/deoxypyridinoline conjugate to bind the conjugate thereto in a manner which leaves the deoxypyridinoline free to competitively bind with labeled anti-deoxypyridinoline antibody when contacted with a fluid test sample in which deoxypyridinoline is present.