Patent ID: 6207396
Filing Date: 2001-03-27
Classification: G01N

Abstract:
An immunoassay method for determining the presence of LSD or LSD metabolites in a sample comprisinga) contacting the sample with an antibody to LSD or LSD metabolites, wherein said antibody is raised to an LSD-protein conjugate having a protein carrier conjugated to said LSD at the N-1 position of said LSD, said LSD-protein conjugate having the structure ##STR8##wherein R1 is an alkyl cycloalkyl, or aryl group having 1 to 10 carbon atoms;wherein R2 is a bond or ##STR9##wherein R3 is an alkyl, cycloalkyl, or aryl group having 2 to 10 carbon atoms;wherein Z is selected from the group consisting of an immunogenic carrier substance, an enzyme donor polypeptide, and a label; andn is 1 to p wherein p is the molecular weight of Z/1000;b) contacting the antibody with said LSD-protein conjugate;c) measuring complexes formed between the conjugate and the antibody by contacting the complexes with a detection reagent, and thereby detecting a first signal generated by the complexes; andd) determining an amount of LSD or LSD metabolites in the sample by comparing the first signal detected to a second signal detected using a known concentration of LSD or LSD metabolites;wherein one or more of the following are characteristics of the antibody:(i) the antibody is less than 1% crossreactive with chlorpromazine;(ii) the antibody is less than 0.1% crossreactive with ergotamine;(iii) the antibody binds the conjugate such that the frequency of false positive reactions with clinical urine samples not containing LSD is less than 4%;(iv) the antibody binds the conjugate such that the median relative level of LSD and LSD metabolites measurable in urine samples taken from a subject 36 hours after administration of LSD is at least 5% of the highest levels of LSD and LSD metabolites measurable in urine samples from the same subject within the first 24 h after the administration; or(v) the antibody is capable of binding the conjugate in a manner that is inhibitable by a glucuronide derivative of LSD.