Patent ID: 6333178
Filing Date: 2001-12-25
Classification: C07K,C12N,C12Q

Abstract:
A method for replicating a DNA molecule having DNA lesion damage, comprising:providing a sample containing a DNA molecule with one or more sites of DNA lesion damage; andcontacting the DNA molecule with a translesion replication DNA polymerase, capable of replicating through DNA lesions and selected from the group consisting of UmuC (DNA polymerase V), a fusion protein of UmuC, a fragment of UmuC, and a prokaryotic homologue of UmuC, a fragment of the prokaryotic UmuC homologue, and a fusion protein of the prokaryotic UmuC homologue, in the presence of a combination of UmuD', RecA and SSB proteins, or in the presence of a combination in which at least one of the UmuD', RecA and SSB proteins is replaced by a functional prokaryotic homologue thereof, nucleoside 5'-triphosphates, and a divalent metal ion to replicate the damaged DNA molecule by replicating through the one or more sites of DNA lesion damage.