Patent ID: 8048633
Filing Date: 2011-11-01
Classification: B01L,B03C,C12N,C12Q

Abstract:
1. A method of performing a nucleic acid amplification assay comprising: (a) combining reagents, DNA polymerase, a target nucleic acid, and a modified primer, (b) cycling the mixture of (a) at said first temperature range to provide multiple copies of an amplicon incorporating said modified primer, wherein said incorporated primer is capable of hybridizing with an oligonucleotide complementary to said single stranded hybridization region in said first elevated temperature range and in said second lower temperature range; (c) reducing the temperature of the mixture to the second temperature range; (d) exposing said mixture to a capture oligonucleotide complementary to said single stranded hybridization region; (e) hybridizing said single stranded hybridization region of said amplicon incorporating said modified primer, with said capture oligonucleotide; and (f) detecting said label associated with said hybridization.