Patent ID: 6538178
Filing Date: 2003-03-25
Classification: C12N

Abstract:
A method of producing genetically transformed plants which have elevated starch content, comprising the steps of:(a) inserting into the genome of a plant cell a recombinant, double-stranded DNA molecule comprising (i) a promoter which functions in plants to cause the production of an RNA sequence in the target plant tissues, (ii) a structural DNA sequence that causes the production of an RNA sequence which encodes a fusion polypeptide comprising an amino-terminal plastid transit peptide and a heterologous ADPglucose pyrophosphorylase enzyme, (iii) a 3â€² non-translated DNA sequence which functions in plant cells to cause transcriptional termination and the addition of polyadenylated nucleotides to the 3â€² end of the RNA sequence; (b) obtaining transformed plant cells; and (c) regenerating from the transformed plant cells genetically transformed plants which have an elevated starch content.