Patent ID: 6569649
Filing Date: 2003-05-27
Classification: A61P,C12N,C12P,Y10S

Abstract:
A method for synthesizing a saccharide composition comprising preselected saccharide units, comprising:(a) contacting an acceptor moiety with a preselected glycosyltransferase to form a acceptor moiety-glycosyltransferase intermediate; (b) contacting the acceptor moiety-glycosyltransferase intermediate with the preselected saccharide donor moiety under conditions suitable to effect glycosyltransferase-catalyzed transfer of a preselected saccharide unit from the preselected saccharide donor moiety to the acceptor moiety to form a product; (c) repeating steps (a) and (b) at least once such that each time said steps are repeated, the product obtained in step (b) functions as the acceptor moiety in step (a), and such that after the last time said steps are repeated, the product obtained in step (b) is the saccharide composition; wherein each preselected glycosyltransferase contacted in step (a) is provided by a method comprising: (i) contacting an immobilized acceptor moiety with a mixture of glycosyltransferases under conditions suitable to effect bonding of any glycosyltransferase specific for the immobilized acceptor moiety, to form a plurality of immobilized acceptor moiety-glycosyltransferase complexes; (ii) eluting a glycosyltransferase from the plurality by contacting the plurality with the donor moiety comprising the preselected saccharide unit under conditions suitable to effect glycosyltransferase-catalyzed transfer of the preselected saccharide unit from the donor moiety to the immobilized acceptor moiety and to release the glycosyltransferase and form an immobilized product; and (iii) collecting the released glycosyltransferase.