Patent ID: 9115358
Filing Date: 2015-08-25
Classification: A61K,C12N,C12P

Abstract:
1. An isolated recombinant cell expressing one or more polypeptides that comprise a moenomycin biosynthesis-related polypeptide selected from the group consisting of: MoeA4 (SEQ ID NO: 29), MoeB4 (SEQ ID NO: 30), MoeC4 (SEQ ID NO: 28), MoeB5 (SEQ ID NO: 27), Moe A5 (SEQ ID NO: 26), MoeD5 (SEQ ID NO: 47), MoeJ5 (SEQ ID NO: 48), MoeE5 (SEQ ID NO: 42), MoeF5 (SEQ ID NO: 36), MoeH5 (SEQ ID NO: 37), MoeK5 (SEQ ID NO: 38), MoeM5 (SEQ ID NO: 39), MoeN5 (SEQ ID NO: 43), MoeO5 (SEQ ID NO: 44), MoeX5 (SEQ ID NO: 46), MoeP5 (SEQ ID NO: 45), MoeR5 (SEQ ID NO: 40), MoeS5 (SEQ ID NO: 41), MoeGT1 (SEQ ID NO: 31), MoeGT2 (SEQ ID NO: 32), MoeGT3 (SEQ ID NO: 33), MoeGT4 (SEQ ID NO: 34), MoeGT5 (SEQ ID NO: 35), and variants thereof having at least about 95% sequence identity to the corresponding natural moenomycin biosynthesis-related polypeptide, wherein the cell is selected from the group consisting of: Streptomyces lividans TK24, E. coli , a mammalian cell, a yeast cell, and an insect cell.