Patent ID: 7880057
Filing Date: 2011-02-01
Classification: A01H,A23L,C07K,C12N

Abstract:
1. A method of modifying a trait of a plant, comprising (a) using Agrobacterium -mediated transformation to stably transform plant cells with a desired polynucleotide, wherein (i) the desired polynucleotide is located within a P-DNA or T-DNA of a vector, and (ii) the vector comprises a cytokinin integration marker gene in its backbone positioned outside of the P-DNA or T-DNA, wherein expression of the integration marker gene alters a morphological feature of a shoot that is regenerated from the transformed cells, (b) regenerating shoots from the cells, (c) identifying shoots, or plants obtained therefrom, that (i) do not have an altered morphological feature compared to shoots or plants regenerated from non-transformed cells, but (ii) do have the desired polynucleotide integrated into their genomes, wherein expression of the desired polynucleotide modifies a trait in the shoot or plant.