Patent ID: 8722365
Filing Date: 2014-05-13
Classification: C07K,C12N,C12P

Abstract:
1. A method of producing sialic acid, comprising the steps of: providing a genetically engineered microbe, wherein the microbe comprises an inactivating mutation in a first DNA molecule in the microbe that encodes a sialic acid transporter; an inactivating mutation in a second DNA molecule in the microbe the encodes a sialic acid aldolase; and a first enzyme-encoding recombinant DNA molecule in the microbe, wherein the first enzyme-encoding recombinant DNA molecule encodes a uridine diphosphate N-acetylglucosamine 2-epimerase; and a recombinant DNA molecule encoding a glucosamine synthase, wherein the recombinant DNA molecule encoding a glucosamine synthase is a glucosamine synthase gene (glmS), and wherein the overexpression of the glmS gene provides increased uridine diphosphate N-acetylglucosamine levels compared with a wild-type strain; and culturing the microbe in the presence of a carbon source.