Patent ID: 6726821
Filing Date: 2004-04-27
Classification: G01N

Abstract:
A precast polyacrylamide gel containing a buffer adapted for use in gel electrophoresis, wherein an electrophoresis buffer solution comprises an aqueous solution containing a tris(hydroxymethyl) aminomethane and an ampholyte, wherein:(1) said tris(hydroxymethyl) aminomethane is present in a concentration of between 0.07 mol/litre and 0.2 mol/litre, (2) glycine and at least one conjugate ampholyte are present, (a) said conjugate ampholyte has a basic dissociation constant of 8.3(b) said conjugate ampholyte is added ranging in amount from 0.1 to 30 mol % with respect to said glycine, and (c) a total concentration of said conjugate ampholyte and said glycine ranges between 0.1 and 0.3 mol/litre; and (3) the pH of said electrophoresis buffer solution is adjusted to a pH of between 6.0 and 6.8.