Patent ID: 6346244
Filing Date: 2002-02-12
Classification: A21D,A23K,A23L,A61K,A61Q,C12N,C12Y,D06M

Abstract:
An isolated polypeptide having protein disulfide isomerase activity, which (a) is encoded by a nucleic acid sequence which hybridizes with (i) the DNA sequence of SEQ ID NO:1 or (ii) the DNA sequence of SEQ ID NO:2, under the following conditions: presoaking in 5Ã—SSC and prehybridizing for 1 h at Ëœ40Â° C. in a solution of 5Ã—SSC, 5Ã—Denhardt's solution, 50 mM sodium phosphate, pH 6.8, and 50 &mgr;g of denatured sonicated calf thymus DNA, followed by hybridization in the same solution supplemented with 50 &mgr;Ci 32P-dCTP labelled probe for 18 h at Ëœ40Â° C. followed by washing three times in 2Ã—SSC, 0.2% SDS at 40Â° C. for 30 minutes; and (b) has an amino acid sequence that is at least 80% homologous to the amino acid sequence of SEQ ID NO:3.