Patent ID: 7090977
Filing Date: 2006-08-15
Classification: C12Q,Y10T

Abstract:
1. A method for detecting nucleic acid hybridization comprising the steps of: (1) fixing probe nucleic acid having thiol group on the position of (2) washing the surface of working electrode to which the probe nucleic acid is fixed, with phosphoric acid solution, followed by immersing into a buffer solution, which contains target nucleic acid having base sequences complementary to the probe nucleic acid, in order to allow hybridization to occur; (3) adding the buffer solution dissolved with intercalators to the hybridized nucleic acid, in order to bind the intercalators to the hybridized double-stranded nucleic acid; (4) removing any intercalators that remain unbound by washing with the buffer solution; (5) placing the working electrode, on which hybridization and the binding of the intercalator occurred, into a measurement container, followed by adding transition metallic complex that is dissolved in the buffer solution, and installing a reference electrode and a counter electrode; and (6) applying a voltage on the electrodes and inducing oxidation-reduction reaction of the transition metallic complex by the binding of the intercalator to the double-stranded nucleic acid, to thereby cause an electrochemiluminescence reaction.