Patent ID: 6468756
Filing Date: 2002-10-22
Classification: A61P,C07K

Abstract:
A process involving competitive binding for identifying a chemical compound which specifically binds to a mammalian SNORF25 receptor which comprises separately contacting cells expressing on their cell surface the mammalian SNORF25 receptor, wherein such cells do not normally express the mammalian SNORF25 receptor, with both the chemical compound and a second chemical compound known to bind to the receptor, and with only the second chemical compound, under conditions suitable for binding of such compounds to the receptor, and detecting specific binding of the chemical compound to the mammalian SNORF25 receptor, a decrease in the binding of the second chemical compound to the mammalian SNORF25 receptor in the presence of the chemical compound being tested indicating that such chemical compound binds to the mammalian SNORF25 receptor; wherein the mammalian SNORF25 receptor is a human SNORF25 receptor that has an amino acid sequence identical to the amino acid sequence shown in SEQ ID NO: 2 or that encoded by the plasmid pEXJT3T7-hSNORF25 (ATCC Accession No. 203495); or a rat SNORF25 receptor that has an amino acid sequence identical to the amino acid sequence shown in SEQ ID NO: 4 or that encoded by the plasmid pcDNA3.1-rSNORF25 (ATCC Accession No. 203494); or a mouse SNORF25 receptor that has an amino acid sequence identical to the amino acid sequence shown in SEQ ID NO: 25 or that encoded by the plasmid pEXJ-mSNORF25-f (ATCC Patent Depository No. PTA-2263).