Patent ID: 9051600
Filing Date: 2015-06-09
Classification: C12Q,C12Y,G01N

Abstract:
1. A method for measuring sphingomyelin in a sample, comprising reacting the sample with a phospholipase D which does not react with sphingomyelin and lysophosphatidylcholine but reacts with phosphatidylcholine; a lysophospholipase or a monoglyceride lipase; and a choline oxidase, eliminating the formed hydrogen peroxide, reacting the resultant with a phospholipase D which does not react with glycerol-3-phosphorylcholine and free fatty acid but reacts with sphingomyelin; and a choline oxidase, measuring the formed hydrogen peroxide, and correlating the formed hydrogen peroxide with a calibration curve to determine sphingomyelin in the sample.