Patent ID: 7074579
Filing Date: 2006-07-11
Classification: G01N,Y10S

Abstract:
1. A two-site immunoassay for the qualitative or quantitative detection of alpha-amylase in a test sample, said immunoassay comprising; (i) exposing said test sample to a first antibody or fragment thereof which specifically or preferentially binds to a first epitope on said alpha-amylase, under conditions permitting binding of said first antibody or fragment thereof to alpha-amylase if present, (ii) subsequently exposing bound alpha-amylase, if any, to a second antibody or fragment thereof which specifically or preferentially binds to a second epitope on said alpha-amylase that is distinct from said first epitope, under conditions permitting binding of said second antibody or fragment thereof to said bound alpha-amylase, and (iii) detecting any binding of said second antibody or fragment thereof to said bound alpha-amylase, wherein either of said first or second epitopes is an epitope consisting of one or more amino acid sequences selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3, and wherein detection of binding of said second antibody or fragment thereof to said bound alpha-amylase qualitatively or quantitatively indicates the presence of alpha-amylase in said test sample.