Patent ID: 6830881
Filing Date: 2004-12-14
Classification: A01K,C12N,G01N

Abstract:
A method for identifying an agent that modulates sphingosine-1-phosphate lyase activity, comprising:(a) contacting a candidate agent with a polypeptide comprising an amino acid sequence selected from the group consisting of: an amino acid sequence set forth in SEQ ID NO:8; and an amino acid sequence having at least 95% identity to a sequence set forth in SEQ ID NO:8; wherein said polypeptide has sphingosine-1-phosphate lyase activity; and wherein the step of contacting is carried out under conditions and for a time sufficient to allow the candidate agent to interact with said polypeptide; and (b) subsequently measuring the ability of said polypeptide to degrade sphingosine-1-phosphate or a derivative thereof, relative to an ability in the absence of said candidate agent, and therefrom identifying an agent that modulates sphingosine-1-phosphate lyase activity.