Patent ID: 8669094
Filing Date: 2014-03-11
Classification: C12N,C12P,C12Y,Y02E,Y02P

Abstract:
1. A recombinant yeast microorganism for producing isobutanol, the recombinant yeast microorganism comprising, an engineered isobutanol biosynthetic pathway, wherein said engineered isobutanol biosynthetic pathway comprises the following substrate to product conversions: (a) pyruvate to acetolactate; (b) acetolactate to 2,3-dihydroxy-isovalerate; (c) 2,3-dihydroxy-isovalerate to α-ketoisovalerate; (d) α-ketoisovalerate to isobutyryl-CoA; and (e) isobutyryl-CoA to isobutyraldehyde, wherein i) the substrate to product conversion of step (a) is performed by an acetolactate synthase enzyme; ii) the substrate to product conversion of step (b) is performed by an acetohydroxy acid isomeroreductase enzyme; iii) the substrate to product conversion of step (c) is performed by an acetohydroxy acid dehydratase enzyme; iv) the substrate to product conversion of step (d) is performed by a branched-chain keto acid dehydrogenase; and v) the substrate to product conversion of step (e) is performed by an acylating aldehyde dehydrogenase enzyme and wherein the recombinant yeast microorganism is substantially free of at least one enzyme having pyruvate decarboxylase activity and, is substantially free of at least one enzyme having glycerol-3-phosphate dehydrogenase activity.