Patent ID: 6391637
Filing Date: 2002-05-21
Classification: A61K,C07K,C12N,Y10S

Abstract:
A method for stimulating hybridoma cells to increase monoclonal antibody secretion, comprising contacting hybridoma cells with a recombinant soluble oligomeric polypeptide comprising a polypeptide encoded by a DNA molecule selected from the group consisting of:(a) a DNA molecule comprising nucleotides 139 through 780 of SEQ ID NO:1; (b) a DNA molecule comprising nucleotides 355 through 780 of SEQ ID NO:1; (c) a DNA molecule comprising nucleotides 184 through 828 of SEQ ID NO:11; (d) a DNA molecule comprising nucleotides 196 through 828 of SEQ ID NO:11; (e) a DNA molecule comprising nucleotides 382 through 828 of SEQ ID NO:11; (f) a DNA molecule comprising nucleotides 403 through 828 of SEQ ID NO:11; and (g) a DNA molecule comprisino nucleotide sequenccs that due to the degeneracy of the genetic code encode the polypeptides encoded by the nucleotides of (a)-(f); wherein the encoded polypeptides of (a)-(g) are capable of stimulating B cell proliferation.