Patent ID: 6287785
Filing Date: 2001-09-11
Classification: G01N,Y10S

Abstract:
A homogeneous enzyme immunoassay process for measuring a concentration of an antigen by determining the change in enzymatic activity caused by a reaction between said antigen and an enzyme-labeled antibody, said process comprising:providing an enzyme-labeled antibody comprising a conjugate of a labeling enzyme and a first monoclonal antibody;contacting said antigen with said enzyme-labeled antibody to obtain a first reaction mixture, said enzyme-labeled antibody recognizing and binding to a first epitope of the antigen;contacting said first reaction mixture with a second monoclonal antibody to obtain a second reaction mixture, said second monoclonal antibody recognizing and binding to a second epitope of said antigen different from the first epitope;contacting said second reaction mixture with a third antibody to obtain a third reaction mixture, said third antibody recognizing and binding to said second monoclonal antibody;contacting said third reaction mixture with a water-insoluble substrate; andmeasuring the change in activity of the enzyme after it has acted on said substrate, and relating the change in the activity of the enzyme to the concentration of the antigen.