Patent ID: 7662562
Filing Date: 2010-02-16
Classification: C12Q

Abstract:
1. A method for detecting the presence of Staphylococcus aureus in a sample, the method comprising: (a) releasing RNA or DNA from at least one microorganism in the sample; (b) contacting the RNA or DNA with at least one capture probe capable of hybridizing to a first target sequence of the RNA or DNA, wherein the contacting is performed under conditions that permit hybridization between the first target sequence and the at least one capture probe to form a RNA or DNA-capture probe hybrid complex, and wherein the at least one capture probe comprises at least one sequence selected from the group consisting of SEQ ID NOs: 1, 2, 61, 62, and 73; and (c) detecting the presence of the RNA or DNA-capture probe hybrid complex by (i) contacting the RNA or DNA with at least one detector probe capable of hybridizing to a second target sequence of the RNA or DNA, wherein the detector probe comprises at least one reporter group and wherein the contacting is performed under conditions that permit hybridization between the second target sequence and the at least one detector probe to form a RNA or DNA-capture probe-detector probe hybrid complex, and wherein the at least one detector probe comprises at least one sequence selected from the group consisting of SEQ ID NOs: 61 and 62; and (ii) detecting the RNA or DNA-capture probe-detector probe hybrid complex by detecting the at least one reporter group wherein the presence of the RNA or DNA-capture probe-detector probe hybrid complex indicates the presence of