Patent ID: 6673351
Filing Date: 2004-01-06
Classification: A61K,A61P,C07K,Y02A

Abstract:
An immunotherapeutic agent capable of eliciting an immune response to result in abatement of the clinical symptoms of psoriasis, said agent comprising a purified protein extract wherein said purified extract is isolated by diethylaminoethyl Sephadex chromatography of a Nonidet P-40 insoluble particulate antigen fraction derived from isolated killed cells of amastigotes from at least one species of the Leishmania genus, said particulate antigen fraction solubilized with 8 M urea and 0.025 M Tris[hydroxymethyl]aminomethane pH 8.3 applied to diethylaminoethyl Sephadex and eluted with a solution comprising 0.1 M. sodium chloride, 8 M urea and 0.025 M. Tris[hydroxymethyl]aminomethane pH 8.3, said purified protein extract consisting of polypeptides having apparent molecular weights after total reduction and allylation of 73, 80 and 82 kDa.