Patent ID: 6900034
Filing Date: 2005-05-31
Classification: C07K

Abstract:
1. Method for the preparation of a purified, recombinant protein variant of a Bet v1 allergen, wherein said variant is fragment A (amino acid 1-74), B (amino acid 75-164), allergic fragments thereof, or multimers of the Bet v1 allergen, in soluble, biologically active form as a physiological medium which is directly suitable for medical use from protein aggregates (inclusion bodies) obtained after bacterial expression which are insoluble in the expression medium, characterized in that the following steps are carried out: (i) uptake of the separated-off insoluble protein aggregates in organic denaturing reagents, (ii) purification of the solution from (i) by means of at least one chromatography step using essentially inorganic unbuffered alkaline, salt containing eluents, and (iii) neutralization of the alkaline solution obtained after the final purification step which contains the dissolved, renatured and biologically active protein.