Patent ID: 6270958
Filing Date: 2001-08-07
Classification: C12N,C12Q,Y10S

Abstract:
A genetically engineered cell for detecting a negative-strand RNA virus, the cell comprising:a polynucleotide encoding a DNA-dependent RNA polymerase;a cDNA comprising a minigenome and a miniantigenome of the negative-strand RNA virus operably linked to a promoter for the DNA-dependent RNA polymerase, wherein the miniantigenome comprises a nucleotide sequence encoding a reporter gene product, and wherein expression of the reporter gene product is dependent upon the presence of the negative-strand RNA virus; andone or more nucleotide sequences encoding each of the nucleocapsid proteins of the negative-strand RNA virus which are necessary and sufficient for replication of minigenome RNA or miniantigenome RNA synthesized by the DNA-dependent RNA polymerase.