Patent ID: 6232066
Filing Date: 2001-05-15
Classification: B01J,C40B

Abstract:
A method of detecting at least one nucleic acid target, comprising contacting a sample which may comprise said target(s) with a nuclease protection fragment(s) specific for and which binds to said target(s), exposing the s ple to a nuclease effective to digest remaining single strand nucleic acid, and then contacting the resultant sample with a combination which comprises, before the addition of said sample,i) a surface comprising multiple spatially discrete regions, at least two of which are substantially identical, each region comprisingii) at least two different anchors, each in association withiii) a bifunctional linker which has a first portion that is specific for the anchor, and a second portion that comprises a probe which is specific for said nuclease protection fragment(s),under conditions effective for said nuclease protection fragment(s) to bind to said combination,and detecting said bound protection fragment(s).