Patent ID: 6750046
Filing Date: 2004-06-15
Classification: C07K,C12N,C12Y

Abstract:
A method for the expression of a thioredoxin or thioredoxin reductase by a host cell said method comprising:a) introducing into a plant, bacterial or yeast host cell a chimeric nucleic acid sequence comprising: 1) a first nucleic acid sequence capable of regulating the transcription in said host cell of 2) a second nucleic acid sequence, wherein said second sequence encodes a fusion polypeptide and comprises (i) a nucleic acid sequence encoding a sufficient portion of an oleosin gene to provide targeting of the fusion polypeptide to a lipid phase linked in reading frame to (ii) a nucleic acid sequence encoding a thioredoxin or thioredoxin reductase; and 3) a third nucleic acid sequence encoding a termination region functional in the host cell; and b) growing said host cell to produce the fusion polypeptide.