Patent ID: 6245296
Filing Date: 2001-06-12
Classification: G01N,Y10S

Abstract:
A flow immunosensor for performing a real-time analysis of a sample which is to be analyzed and which is not yet present, said flow immunosensor consisting essentially of;an exchanger including:a chamber having an inlet port for sample introduction and an outlet port,a support medium, comprising a bed having a volume of 0.1 to 0.5 ml, in the chamber,an antibody immobilized on the medium wherein the antibody recognizes with specificity and sensitivity a target,a labelled antigen complexed to said immobilized antibody and capable of being displaced by the target, said labelled antigen saturating the antigen-binding sites of said antibody;a detection apparatus connected to the exchanger via the outlet port; anda flow means adapted for flowing liquid through the bed at a rate of 0.1 to 2.0 ml/min, thereby providing a target molecule in said sample insufficient time in the vicinity of said antigen-binding site and said labelled antigen for equilibration.