Patent ID: 8044188
Filing Date: 2011-10-25
Classification: A61K,A61P,C07K,C12N,C12Y,Y02A

Abstract:
1. An isolated nucleic acid molecule encoding a Clostridial propeptide, wherein the propeptide comprises: a light chain region; a heavy chain region, wherein the light and heavy chain regions are linked by a disulfide bond; an intermediate region connecting the light and heavy chain regions and comprising a highly specific protease cleavage site, wherein said highly specific protease cleavage site has three or more specific adjacent amino acid residues that are recognized by the highly specific protease in order to enable cleavage; a signal peptide coupled to the light chain region, wherein the signal peptide is suitable to permit secretion of the propeptide from a eukaryotic cell to a medium; and an affinity tag located between the signal peptide and the light chain region, wherein the affinity tag has a sequence of SEQ ID NO: 45.