Patent ID: 6221642
Filing Date: 2001-04-24
Classification: C12N

Abstract:
A process for reconstituting the .gamma.-less Pol III* subassembly from E. coli from subunits .tau., .alpha., .epsilon., .theta., .delta., .delta.', .chi., and .PSI., which comprises:combining .tau., .alpha., .epsilon., .theta., .delta., .delta.', .chi., and .PSI., under conditions effective to form said .gamma.-less Pol III* subassembly, wherein said combining comprises:combining .chi., .PSI., .delta., and .delta.' under conditions effective to form a .chi..PSI..delta..delta.' complex;combining said .chi..PSI..delta..delta.' complex with .tau. under conditions effective to form a .tau..chi..PSI..delta..delta.' complex;combining said .tau..chi..PSI..delta..delta.' complex with .alpha., .epsilon., .theta.; andrecovering said .gamma.-less Pol III* subassembly.