Patent ID: 6066452
Filing Date: 2000-05-23
Classification: C12Q,G01N

Abstract:
A method for isolating DNA-binding proteins, comprising:(a) mixing (i) a set of oligonucleotide duplexes, wherein said duplexes comprise 5' and 3' sequences that will hybridize to primers for amplification and a randomly generated internal sequence of nucleotides, (ii) an inhibitor of non-sequence specific binding of DNA-binding proteins to the oligonucleotide duplexes and (iii) a sample containing a mixture of DNA-binding proteins under conditions in which the concentration of oligonucleotide duplexes is at or below the average Kd value for specific binding between the oligonucleotide duplexes and the DNA-binding proteins;(b) separating unbound oligonucleotide duplexes from oligonucleotide duplexes complexed with the DNA-binding proteins on the basis of differences in physical or biochemical properties;(c) recovering the DNA-binding proteins from the complexes.