Patent ID: 6794502
Filing Date: 2004-09-21
Classification: C07H,Y02P

Abstract:
A method for improving deprotection of an acid-labile 5â€²-OH protecting group-containing oligonucleotide comprising:determining the half-life for said acid-labile 5â€²-OH protecting group-containing oligonucleotide in an acid solution capable of removing the acid-labile 5â€²-OH protecting group from said oligonucleotide, and reacting said acid-labile 5â€²-OH protecting group-containing oligonucleotide in said acid solution for approximately 5 to approximately 20 half-lives; wherein said acid solution comprises acetic acid, dichloracetic acid, trichloroacetic acid, zinc bromide or boron trifluoride etherate.