Patent ID: 6150115
Filing Date: 2000-11-21
Classification: G01N,Y10S

Abstract:
A method for quantitatively determining heparan sulfate contained in a specimen solution, which comprises the steps of:(a) contacting the specimen solution with an anti-heparan sulfate antibody immobilized on a solid phase, wherein said anti-heparan sulfate antibody exhibits no cross-reactivity with hyaluronic acid;(b) subjecting heparan sulfate contained in the specimen solution to antigen-antibody reaction with the anti-heparan sulfate antibody on the solid phase to form an antigen-antibody complex;(c) labeling the antigen-antibody complex with a labeling substance;(d) removing residues other than the labeled antigen-antibody complex from the solid phase;(e) detecting the labeling substance as an index of a quantity of the labeled antigen-antibody complex on the solid phase; and(f) calculating the quantity of heparan sulfate contained in the specimen solution based on a calibration curve obtained using a standard solution with the labeling substance.