Patent ID: 6030773
Filing Date: 2000-02-29
Classification: G01N

Abstract:
A method of assaying for antibodies to human double-stranded DNA in a liquid, human-serum specimen, said method comprising the steps of:forming a first incubation mixture of said specimen and a solid phase immunoadsorbent having immobilized thereon animal anti-human IgG antibody including F(ab')2 fragment specific for IgG Fc;incubating said first mixture under conditions and for a period of time sufficient for Fc in human IgG in said specimen to become bound by said F(ab')2 fragment in said animal anti-IgG antibody;forming a second incubation mixture of said first mixture and digoxigenin-labelled synthetic double-stranded DNA under such conditions and for a period of time sufficient to bind to said immunoadsorbent any anti-DNA antibodies which may be present in the serum so as to form a double-stranded DNA antibody that has substantially no single stranded or denatured components;detecting the amount of digoxigenin labelled synthetic double-stranded DNA bound to said immunoadsorbent; andrelating the amount of bound labelled synthetic double-stranded DNA detected to a predetermined quantitative relationship between the amount of labelled double-stranded DNA and the amount of animal anti-human IgG antibody to determine the amount of human IgG in said specimen.