Patent ID: 6140471
Filing Date: 2000-10-31
Classification: B01J,C07K,C12N

Abstract:
A specific binding pair memberwhich is a single chain specific binding pair member comprising a first polypeptide chain component and a second polypeptide chain component and which is specific for a complementary specific binding pair member of interest, produced by a method having the following steps:(a) introducing into prokaryotic host cells(i) first vectors comprising nucleic acid encoding a genetically diverse population of said first polypeptide chain component fused to a component of a secreted replicable genetic display package for display of said polypeptide chain components at the surface of replicable genetic display packages; and(ii) second vectors comprising nucleic acid encoding a genetically diverse population of said second polypeptide chain components;said first vectors being packaged in infectious replicable genetic display packages and their introduction into prokaryotic host cells being by infection into prokaryotic host cells harbouring said second vectors, orsaid second vectors being packaged in infectious replicable genetic display packages and their introduction into prokaryotic host cells being by infection into host cells harbouring said first vectors; and(b) causing or allowing recombination between said first and second vectors within said prokaryotic host cells, the recombination being promoted by inclusion in said first and second vectors of sequences at which site-specific recombination occurs, which sequences at which site-specific recombination occurs are derived from a loxP sequence, resulting in recombinant vectors each of which comprises nucleic acid encoding a said single chain specific binding pair member comprising a said first polypeptide chain component and a said second polypeptide chain component and an amino acid sequence encoded by a sequence provided by recombination between said sequences at which site-specific recombination occurs, and capable of being packaged into a replicable genetic display packages using said replicable genetic display package component;(c) expressing said single chain specific binding pair members, producing replicable genetic display packages which display at their surface said single chain specific binding pair members and which each comprise nucleic acid encoding a said single chain specific binding pair member;(d) selecting by binding with said complementary specific binding pair member of interest one or more single chain specific binding pair members specific for said complementary specific binding pair member of interest, each single chain specific binding pair member thus selected being associated in its respective replicable genetic display package with nucleic acid encoding that single chain specific binding pair member;(e) obtaining nucleic acid encoding a single chain specific binding pair member from its replicable genetic display package displaying a specific binding pair member selected in step (d);(f) producing, by expression of encoding nucleic acid in a recombinant host organism, a single chain specific binding pair member comprising a first polypeptide chain component and a second polypeptide chain component and an amino acid sequence encoded by a sequence provided by recombination between said sequences at which site-specific recombination occurs and specific for said complementary specific binding pair member of interest, which single chain specific binding pair comprises a first polypeptide chain component which is as encoded by nucleic acid encoding a said first polypeptide chain component of a specific binding pair member selected in step (d) or is a derivative thereof by way of addition, deletion, substitution or insertion of one or more amino acids or by linkage of another molecule, and a second polypeptide chain component which is as encoded by nucleic acid encoding a said second polypeptide chain component of a specific binding pair member selected in step (d) or is a derivative thereof by way of addition, deletion, substitution or insertion of one or more amino acids or by linkage of another molecule.