Patent ID: 7422850
Filing Date: 2008-09-09
Classification: C12Q

Abstract:
1. A method of detecting a target nucleic acid in a sample, the method comprising: a) contacting the sample with a nucleic acid polymerase; a first oligonucleotide primer comprising a sequence complementary to a first portion of the target nucleic acid; a second oligonucleotide primer comprising a first region and a second region, the first region comprising a sequence complementary to a second portion of the target nucleic acid and the second region comprising a non-natural base; b) amplifying the target nucleic acid, if present in the sample, using the first and second oligonucleotide primers in the presence of a labeled reporter to generate an amplification product, wherein the labeled reporter comprises a non-natural base which is incorporated into the amplification product opposite the non-natural base present in the second region of the second oligonucleotide primer; and c) observing a signal from the labeled reporter during amplification and correlating the incorporating of the reporter with the presence of the target nucleic acid in the sample.