Patent ID: 7846666
Filing Date: 2010-12-07
Classification: C12N,C12P,C12Q

Abstract:
1. A method of selectively generating multiple copies of a polynucleotide sequence of or complementary to target RNA which is in a sample comprising DNA, said method comprising the steps of: (a) hybridizing to the target RNA in said sample a first primer comprising a sequence (A) that is not complementary to the target RNA, and a sequence (B) at the 3′-end which hybridizes to the target RNA; (b) extending the first primer with at least one enzyme comprising RNA-dependent DNA polymerase activity in the presence of at least one compound comprising DNA-dependent DNA polymerase inhibitor activity, whereby DNA-dependent DNA polymerase activity of the enzyme comprising RNA-dependent DNA polymerase activity is inhibited by said compound, whereby a complex comprising a primer extension product and the target RNA is produced, whereby the first primer extension product comprises a sequence (Y) that is complementary to the target RNA and comprises sequence (A); (c) disabling or removing at least one compound comprising DNA-dependent DNA polymerase inhibitor activity; and (d) producing multiple copies of a polynucleotide sequence complementary to the target RNA and/or complementary to sequence (Y) using sequence (A).