Patent ID: 6444788
Filing Date: 2002-09-03
Classification: A61P,C07K,C12N,C12Y

Abstract:
A process for purifying a glucagon-like peptide 1 (GLP-1) from a mixture comprising said GLP-1 peptide and impurities, said method comprising:eluting said GLP-1 peptide and said impurities of said mixture from a cation exchange chromatography matrix using a solution comprising an organic modifier, water, optionally a salt component, and optionally a buffer, with a linear or step gradient or isocratically in salt component and/or with a linear or step pH-gradient or at a constant pH-value, wherein the pH-gradient or pH-value should be in the range where said GLP-1 peptide has a positive local or overall net charge different from the local or overall positive net charge of said impurities so as to remove said impurities.