Patent ID: 7011955
Filing Date: 2006-03-14
Classification: B01L,C12Q

Abstract:
1. A method for quantitative or qualitative determination of an analyte comprising: (a) incubating a sample containing the analyte with a predetermined amount of labeled competitive substance and a solid phase coated with a quenching substance, wherein the solid phase further comprises an analyte-specific bonding partner immobilized thereto, such that the analyte and the labeled competitive substance compete for binding to the analyte-specific bonding partner, wherein the quenching substance suppresses signal from the labeled competitive substance bound to the solid phase; (b) exciting the sample so as to generate signal from unbound labeled competitive substance; and (c) measuring the signal only generated from the unbound labeled competitive substance in a volume segment of liquid phase, thereby quantitatively or qualitatively determining the analyte, wherein the determination of the analyte is performed or effected without physically separating the inbound and bound labeled competitive substance.