Patent ID: 6518418
Filing Date: 2003-02-11
Classification: C07H,C12P

Abstract:
A process for preparing a protected nucleoside monophosphite-glycoside comprising the following steps:Step A: providing a sugar having an unprotected anomeric carbon but otherwise having protected side groups; Step B: providing a phosphoramiditing agent; Step C: providing a nucleoside having an unprotected ribose hydroxyl oxygen but otherwise having protected side groups; then Step D: forming a sugar phosphoramidite by reacting the sugar of said Step A with the phosphoramiditing agent of said Step B under for forming a glycosidic bond between the anomeric carbon of the sugar of said Step A and the phosphoramiditing agent of said Step B; and then Step E: reacting the sugar phosphoramidite of said Step D with the nucleoside of said Step C for forming the protected nucleoside monophosphite-glycoside, wherein: the protected side groups of said Step A being protective against reactivity in said Step D with the phosphoramiditing agent of said Step B.