Patent ID: 8173443
Filing Date: 2012-05-08
Classification: A61K,G01N

Abstract:
1. A protein profiling method of detecting multiple Lysosomal Storage Disease (“LSD”) target antigens in a dried blood or plasma sample, the protein profiling method comprising: (a) determining target quantities of α-iduronidase and α-glucosidase from a target biological dried blood or plasma sample of a human subject; (b) determining a target quantity of either LAMP-1 or saposin C from the target biological dried blood or plasma sample of the human subject; (c) assigning a set of adjusted target quantities by calculating a set of target proportions between the target quantities of α-iduronidase and α-glucosidase and the target quantity of either LAMP-1 or saposin C; (d) obtaining reference quantities of α-iduronidase and α-glucosidase from a reference biological dried blood or plasma sample from a reference human subject, or group of reference human subjects, having a known LSD pre-clinical or clinical status; (e) obtaining a reference quantity of either LAMP-1 or saposin C from the reference biological dried blood or plasma sample from the reference human subject, or group of reference human subjects, having a known LSD pre-clinical or clinical status; (f) assigning a set of adjusted reference quantities by calculating a set of reference proportions between the reference quantities of α-iduronidase and α-glucosidase and the reference quantity of either LAMP-1 or saposin C; and (g) determining a deviation of the set of adjusted target quantities compared to the set of adjusted reference quantities;