Patent ID: 7202028
Filing Date: 2007-04-10
Classification: C12Q

Abstract:
1. A method of detecting two different target molecules through a single common electrode, comprising the steps of: (a) providing a conductive oxidation-reduction reaction detection electrode; (b) contacting a sample suspected of containing a first and second target molecule to said detection electrode under conditions in which said first and second target molecules are deposited on said detection electrode, wherein said first target molecule comprises a first preselected label, said second target molecule comprises a second preselected label, and said first and second preselected labels are different; (c) simultaneously contacting to said electrode (i) a first transition metal complex that oxidizes said first preselected label in an oxidation-reduction reaction to cause a first oxidation-reduction reaction between the first transition metal complex and the first preselected label and (ii) a second transition metal complex that oxidizes said first and second preselected labels in an oxidation-reduction reaction to cause a second oxidation-reduction reaction between the second transition metal complex and the second preselected label, from which preselected labels there is electron transfer to the corresponding transition metal complexes resulting in regeneration of the reduced form of the transition metal complex as part of a catalytic cycle, with said first and second oxidation-reduction reactions producing different detectable signals; (d) detecting the presence of said first target molecule by detecting said first oxidation-reduction reaction; and (e) detecting the presence of said second target molecule by detecting said second oxidation-reduction reaction.