Patent ID: 8288611
Filing Date: 2012-10-16
Classification: C12N,Y02A

Abstract:
1. A method of producing a transgenic monocot plant comprising the steps of: (1) selecting a nucleic acid encoding an alanine aminotransferase, (2) selecting a promoter that is operable in a monocot plant, wherein the promoter is a rice antiquitin promoter comprising SEQ ID NO:1 or a sequence having 99.9% sequence identity to SEQ ID NO:1, (3) coupling the selected nucleic acid to the selected promoter to form a genetic construct, (4) transforming a monocot plant cell with the genetic construct to form a transformed cell, and (5) growing a transgenic monocot plant from the transformed cell to produce a transgenic monocot plant, wherein expression of said nucleic acid in said monocot plan causes an increase in plant dry weight biomass when expressed in a transgenic monocot plant compared to the plant dry weight biomass of a comparable monocot plant not expressing said construct when the plant expressing the construct and the plant not expressing the construct are grown under conditions that are limiting nitrogen conditions for the plant not expressing the construct.