Patent ID: 8492120
Filing Date: 2013-07-23
Classification: C07K,C12N

Abstract:
1. A process for producing Granulocyte Colony Stimulating Factor (G-CSF) comprising the steps of: i) inoculating a fermenter with a suitable ii) carrying out batch or and fed batch mode with suitable culture broth to increase biomass, iii) adding pre-induction medium to the culture broth, and iv) adding production medium to the culture broth in a substrate limiting fed bath mode in which multiple inductions of the nucleic acid sequence encoding G-CSF are carried out to produce G-CSF, v) wherein the culture broth comprises at least 5 g/L thiamine, wherein the production medium has a final concentration of potassium (K) from 60 mM to 180 mM, a final concentration of sodium (Na) from 60 mM to 300 mM, and a final concentration of magnesium (Mg) from 150 mM to 250 mM.