Patent ID: 6635423
Filing Date: 2003-10-21
Classification: C12Q,Y10S

Abstract:
A method for identifying a plurality of genes that are differentially expressed between dissimilar biological samples for use in an informative array, comprising:providing a first set of heterogeneous nucleic acid probes derived from a first biological sample; providing a second set of heterogeneous nucleic acid probes derived from a second biological sample; hybridizing a nucleic acid array comprising a plurality of sequences derived from genes of a biological process with the first set of probes and determining a first level of expression for sequences of the array; hybridizing the array with said second set of probes and determining a second level of expression for sequences of the array; identifying a plurality of genes that are differentially expressed in said biological process by comparing the first level of expression with said second level of expression for hybridized sequences; and establishing a ranking of the identified genes by a step selected from the group of steps consisting of: determining an absolute value of the difference between the first level of expression and the second level of expression, and ranking genes having a higher difference over genes having a lower difference; and determining a standard deviation of the difference between the first level of expression and the second level of expression, and ranking genes having a higher standard deviation over genes having a lower standard deviation.