Patent ID: 8614095
Filing Date: 2013-12-24
Classification: C12N,G01N,Y10T

Abstract:
1. A method for the preparation of a cell composition, comprising the steps of: (a) Providing a tumor cell sample; (b) Optionally culturing the cells provided in (a) in a culture medium; (c) Isolating in a sub-sample the cells which present autofluorescence emission detected at or about 520 nm upon laser beam excitation at a wavelength of or about 488 nm by fluorescence activated cell sorting, from the cells provided under step (a) or (b); (d) Isolating in another sub-sample by fluorescence activated cell sorting, the cells which are not fluorescent under step (c) and which present a positive shift in the fluorescence detected at >630 nm; (e) Excluding dead cells from each of the isolated cell sub-sample obtained under steps (c) and (d); (f) Pooling the cell sub-sample obtained under step (c) after treatment under step (e); (g) Pooling the cell sub-sample obtained under step (d) after treatment under step (e).