Patent ID: 8802413
Filing Date: 2014-08-12
Classification: A61K,A61P,C12N,C12Y,Y02A

Abstract:
1. A method for producing a modified cytotoxic ribonuclease, comprising: a) producing a cytotoxic ribonuclease from cells transformed with an expression vector encoding a modified cytotoxic ribonuclease; and b) purifying said modified cytotoxic ribonuclease; wherein said modified cytotoxic ribonuclease comprises an amino acid sequence which differs from the amino acid sequence of SEQ ID NO:1 solely by four to seven amino acid substitutions, wherein said four to seven amino acid substitutions are located at positions of SEQ ID NO:1 selected from the group consisting of 7, 31, 38, 39, 67 and 85-94, wherein at least one of the four to seven amino acid substitutions is at a position corresponding to any one of positions 85-94 of SEQ ID NO: 1, wherein at least three of the four to seven amino acid substitutions are at positions corresponding to positions 7, 31, 38, 39, or 67 of SEQ ID NO:1.