Patent ID: 8137971
Filing Date: 2012-03-20
Classification: A61P,C12N

Abstract:
1. A method of preparing a population of viable late stage human motor neuron progenitor cells comprising: (a) obtaining population of human late stage motor neuron progenitor cells having a purity of greater than about 65% late stage motor neuron progenitor cells, wherein the population is cultured on an adherent substrate and provides a layer of motor neuron progenitor cells; (b) dissociating the layer of motor neuron progenitor cells cultured on the adherent substrate to provide a cell suspension; (c) allowing the cell suspension to rest in a non-adherent environment for a period of time sufficient for floating microspheres comprising viable cells to form; (d) selecting the floating microspheres comprising viable cells; and (e) collecting the microspheres to provide a population of viable late stage human motor neuron progenitor cells, wherein the purity of the cells is greater in step e than in step a, further comprising enzymatically dissociating the layer of motor neuron progenitor cells, and washing the cell suspension after dissociating.