Patent ID: 7851160
Filing Date: 2010-12-14
Classification: C12Q

Abstract:
1. A method for electrochemically determining the amount of double-stranded DNA, which comprises adding, to a solution containing double-stranded DNA, a substance capable of binding to the double-stranded DNA in an excess amount relative to the double-stranded DNA so as to react with each other, and then electrochemically determining the residual amount of the free double-stranded DNA binding substance that fails to bind to the double-stranded DNA so as to determine the amount of the double-stranded DNA contained in the reaction solution, in which the reaction solution contains, as a buffering substance, a substance having a property of allowing an oxidation wave potential of a potential-current curve for the double-stranded DNA binding substance determined in a reaction solution containing the buffering substance to change depending upon concentration of the free double-stranded DNA binding substance in the reaction solution.