Patent ID: 7306912
Filing Date: 2007-12-11
Classification: C12N

Abstract:
1. A method for separating non-circular nucleic acid fragments from a nucleic acid mixture, dependent on their length and conformation, comprising: providing a mixture of nucleic acid fragments; loading a sample of said mixture in a gel electrophoresis apparatus and electrophoresing in a first dimension said sample through a polyacrylamide gel matrix under non-denaturing electrophoresis conditions such that said fragments are separated dependent on conformation and length; electrophoresing said sample in the same gel matrix in a second dimension under a second set of electrophoresis conditions in the presence of a chemical agent and optionally combined with altering a physical agent within the gel matrix which agent/s is/are capable of reducing conformational differences between nucleic acid fragments, such that said fragments are separated in said second dimension dependent on length.