Patent ID: 8470586
Filing Date: 2013-06-25
Classification: B01F,B01L,C12N,C12Q,F16K

Abstract:
1. A microfluidic device, comprising: a processing region having a vertical central axis therethrough, the processing region comprised of an inlet, a retention member, a filter, and an outlet; said retention member being configured to preferentially retain one or more polynucleotides in a sample as compared to polymerase chain reaction inhibitors in the sample, wherein said retention member comprises a plurality of polynucleotide binding particles, and said plurality of binding particles having surfaces that comprise a poly-cationic polyamide bound thereto, further wherein the retention member is configured to retain polynucleotides from a sample preferentially to inhibitors in the sample as the sample passes through the retention member from the processing region inlet to the processing region outlet; wherein the vertical central axis of the processing region is perpendicular to a horizontal plane of the microfluidic device; wherein the processing region outlet is disposed along the vertical central axis above the processing region inlet; wherein the filter is configured to prevent the plurality of binding particles from passing downstream of the processing region; a device inlet in communication with, and upstream of, the processing region; a device outlet in communication with, and downstream of, the processing region; and a detection region in fluid communication with and downstream of the retention member.