Patent ID: 6127121
Filing Date: 2000-10-03
Classification: C12Q

Abstract:
A method for distinguishing polynucleotides with related sequences, the method comprising the following steps:(a) providing an oligonucleotide having a defined sequence, wherein one or more purine residues of the oligonucleotide are substituted by a pyrazolo[3,4-d]pyrimidine;(b) providing at least two polynucleotides, each of which comprises a target sequence, wherein one of the polynucleotides has a target sequence that is perfectly complementary to the oligonucleotide and at least one other of the polynucleotides has a related target sequence;(c) separately incubating each of the polynucleotides with the oligonucleotide under hybridization conditions; and(d) determining the degree of hybridization between the oligonucleotide and each of the polynucleotides,wherein the polynucleotides are distinguished by different degrees of hybridization with the oligonucleotide.