Patent ID: 6156953
Filing Date: 2000-12-05
Classification: C07K,C12N,Y02A

Abstract:
A method of preparing a transgenic cell comprising the steps of:a) obtaining a nucleic acid molecule comprising Aribidopsis thailiana centromere DNA having the following characteristics:1) mapping to a location on an Aribidopsis thailiana chromosome defined by a pair of genetic markers selected from the group consisting of UFO and GAPB, m246 and m216, GL1 and TOPP5, GA1 and nga8, and nga76 and PhyC; and2) sorts DNA to the spindle poles in meiosis 1 in a pattern indicating the disjunction of homologous chromosomes;b) preparing a recombinant construct comprising said nucleic acid molecule; andc) transforming a recipient cell with said recombinant construct.