Patent ID: 6100071
Filing Date: 2000-08-08
Classification: A61K,A61P,C07K

Abstract:
A VEGF receptor protein that is capable of binding to VEGF and thereby exerting an inhibitory effect thereon, said VEGF receptor protein comprising tyrosine kinase receptor-derived immunoglobulin-like domains 1, 2 and 3, wherein said tyrosine kinase receptor-derived immunoglobulin-like domain 1 comprises an amino acid sequence selected from the group consisting of:(a) amino acids 32-128 of the flt-1 tyrosine kinase receptor shown in FIG. 1 (SEQ ID NO:1);(b) an amino acid sequence consisting essentially of the Ig-like domain 1 of the flt-1 tyrosine kinase receptor;(c) amino acids 32-118 of the KDR tyrosine kinase receptor shown in FIG. 1 (SEQ ID NO:2); and(d) an amino acid sequence consisting of the Ig-like domain 1 of the KDR tyrosine kinase receptor;wherein said tyrosine kinase receptor-derived immunoglobulin-like domain 2 comprises an amino acid sequence selected from the group consisting of:(e) amino acids 134-226 of the flt-1 tyrosine kinase receptor shown in FIG. 1 (SEQ ID NO:1);(f) an amino acid sequence consisting essentially of the Ig-like domain 2 of the flt-1 tyrosine kinase receptor;(g) amino acids 124-220 of the KDR tyrosine kinase receptor shown in FIG. 1 (SEQ ID NO:2); and(h) an amino acid sequence consisting essentially of the Ig-like domain 2 of the KDR tyrosine kinase receptor; andwherein said tyrosine kinase receptor-derived immunoglobulin-like domain 3 comprises an amino acid sequence selected from the group consisting of:(i) amino acids 232-331 of the flt-1 tyrosine kinase receptor shown in FIG. 1 (SEQ ID NO:1);(j) an amino acid sequence consisting essentially of the Ig-like domain 3 of the flt-1 tyrosine kinase receptor;(k) amino acids 226-327 of the KDR tyrosine kinase receptor shown in FIG. 1 (SEQ ID NO:2); and(l) an amino acid sequence consisting essentially of the Ig-like domain 3 of the KDR tyrosine kinase receptor; andwherein said VEGF receptor protein possesses at least one of (a), (b), (e), (f), (i) or (j) and at least one of (c), (d), (g), (h), (k) or (l).