Patent ID: 9175322
Filing Date: 2015-11-03
Classification: C12N,C12P

Abstract:
1. A biological culture comprising isolated stable mucoid Pseudomonas aeruginosa bacteria that produce alginate, wherein the bacteria are genetically modified Pseudomonas aeruginosa bacteria having increased protease activity of a ClpP, ClpP2, or ClpX polypeptide as compared to wild-type Pseudomonas aeruginosa bacteria that is the result of transformation of the Pseudomonas aeruginosa bacteria with an exogenous DNA encoding the ClpP, ClpP2, or ClpX polypeptide to overexpress the ClpP, ClpP2, or ClpX polypeptide as compared to wild-type Pseudomonas aeruginosa bacteria and wherein the bacteria further have at least one characteristic selected from the group consisting of: (i) increased positive regulator activity of a MucE polypeptide as compared to wild-type (ii) decreased negative regulator activity of a MucA polypeptide as compared to wild-type (iii) lack of expression of a lipopolysaccharide do not express at least one endotoxin biosynthesis gene.