Patent ID: 6210884
Filing Date: 2001-04-03
Classification: C12Q

Abstract:
A method of amplifying nucleic acid sequences, the method comprising,(a) mixing a rolling circle replication primer with one or more amplification target circles, to produce a primer-ATC mixture, and incubating the primer-ATC mixture under conditions that promote hybridization between the amplification target circles and the rolling circle replication primer in the primer-ATC mixture,wherein the amplification target circles each comprise a single-stranded, circular DNA molecule comprising a primer complement portion, and wherein the primer complement portion is complementary to the rolling circle replication primer, andwherein at least one of the amplification target circles is tethered to a specific binding molecule so that the amplification target circle can rotate freely, and(b) mixing DNA polymerase with the primer-ATC mixture, to produce a polymerase-ATC mixture, and incubating the polymerase-ATC mixture under conditions that promote replication of the amplification target circles,wherein replication of the amplification target circles results in the formation of tandem sequence DNA.