Patent ID: 7955794
Filing Date: 2011-06-07
Classification: C12Q

Abstract:
1. A multiplex method for determining whether a sample contains at least 100 different target sequences, comprising: a) providing a sample which may contain at least 100 different single-stranded target sequences attached to a first solid support; b) contacting said target sequences with a probe set comprising more than 100 different single-stranded probes, wherein each of said more than 100 different probes comprises: i) a first universal priming site, wherein each of said more than 100 different probes has identical universal priming sites, and ii) a target specific domain, such that different double-stranded hybridization complexes are formed, each of the different hybridization complexes comprising one of said more than 100 different single-stranded probes and one of the different single-stranded target sequences from the sample; c) removing unhybridized probes; d) contacting said probes of the hybridization complexes with a first enzyme and forming different modified probes; e) contacting said modified probes with: i) at least a first primer that hybridizes to said universal priming site; ii) NTPs; and iii) an extension enzyme; f) immobilizing said different amplicons to a second solid support, and g) detecting said different amplicons immobilized to said second solid support, thereby determining whether the sample contains at least 100 different target sequences.