Patent ID: 8547533
Filing Date: 2013-10-01
Classification: C07K,C09B,G01N,Y10T

Abstract:
1. A super-resolution method for irradiating a selected region of a target material comprising a composite probe of, wherein the composite probe comprises a donor moiety linked to an acceptor moiety with a linker, wherein the donor moiety produces a donor moiety emission when excited to a donor excited state by a first wavelength of incident radiation, and the acceptor moiety is effective to couple the donor and acceptor electromagnetically and quench at least a portion of the donor moiety emission when the acceptor moiety is separately excited to an acceptor excited state by a second wavelength of incident radiation, the method comprising: producing an exciting light beam in an optical device having a spot closely approximating its point spread function, the exciting light beam having a first wavelength in a region effective to excite the donor moiety of the composite probe to a donor excited state to produce a donor emission; producing a quenching light beam in the optical device, the quenching light beam having a second wavelength effective to excite the acceptor moiety of the composite probe to an acceptor excited state, wherein the acceptor moiety in the acceptor excited state is an excited quencher that quenches in the donor emission region, directing the exciting light beam and the quenching light beam of radiation to overlap in the selected region of the target material to quench at least a portion of the donor emission of the donor moiety in the selected region, wherein the quenching produces a modified point spread function in the optical device that is narrower than the point spread function in the absence of the quenching beam, and producing an image of the selected region of the target material, fabricating a structure in the selected region of the target material, etching the selected region of the target material, or producing a perturbation in the selected region of the target material.