Patent ID: 7229769
Filing Date: 2007-06-12
Classification: C12Q

Abstract:
1. A method of determining activity of a protease, comprising: (a) providing a protease substrate comprising a protein moiety attached to a nucleic acid moiety and a ligand moiety; (b) contacting said protease substrate with a protease under conditions wherein said protease catalyzes cleavage of said protein moiety, thereby producing a proteolytic product wherein said nucleic acid moiety is separated from at least a portion of said protein moiety and said ligand moiety; (c) contacting said proteolytic product with a receptor under conditions wherein said ligand moiety binds to said receptor to form a complex; (d) separating said complex from said nucleic acid moiety, wherein said receptor is attached to a solid support, thereby forming a separation product comprising said nucleic acid moiety; (e) contacting said separation product with a probe nucleic acid under conditions wherein said nucleic acid moiety hybridizes to a complementary sequence of said probe; and (f) detecting hybridization of said separation product to said probe, thereby determining activity of said protease.