Patent ID: 6242581
Filing Date: 2001-06-05
Classification: B01D,B01J,C07K,C12N,G01N

Abstract:
An immobilized metal ion affinity chromatography purification method for purification of recombinant proteins, said method comprising:a) providing carboxymethylated aspartate ligand complexed with a transition metal ion in a 2+ oxidation state, having a coordination number of 6;b) loading a mixture of cell lysate comprising a recombinant protein having a polyhistidine tail to bind with said ligand; andc) eluting said recombinant protein with a suitable elutant to obtain a purified recombinant protein, wherein said transition metal-complexed carboxymethylated aspartate ligand forms a carboxymethylated aspartate chelating matrix which comprises said transition metal and a polymer matrix, wherein said carboxymethylated aspartate chelating matrix has the structure ##STR2##whereinR1--R2--R3=H;M=transition metal ion in a 2+ oxidation state with a coordination number of 6;R4=a linking arm connecting the methylene carbon atom of the carboxymethyl group of Cm-Asp with R5;R5=a functional linking group through with CM-Asp linking arm R4 is connected to R6; andR6=a polymer matrix.