Patent ID: 8119345
Filing Date: 2012-02-21
Classification: C12Q,G16B

Abstract:
1. A method for genotyping in typing assays applied to a biological sample, the sample containing a nucleic acid target of interest, the target nucleic acid being susceptible to having a single nucleotide polymorphism (SNP), comprising the steps of: performing a real-time amplification of the target, generating multiple copies of amplicons, in the presence of at least two different labeled probes, each probe allowing real-time detection at an SNP position of both a wildtype and at least one possible mutation, actively calculating a discriminatory variable value based on signals of each combination of the at least two different labeled probes, and discriminating between the genotypes based on the discriminatory variable value, wherein: when the SNP is a bi-allelic SNP, the discriminatory variable value for a heterozygous genotype is a ratio selected from the group consisting of (k the discriminatory variable value for a homozygous mutant genotype is a ratio of k the discriminatory variable value for a homozygous wildtype is a ratio of k when the SNP is a tri-allelic SNP, the discriminatory variable value for an AAA genotype is a ratio of k the discriminatory variable value for an AAG genotype is a ratio selected from the group consisting of (2k the discriminatory variable value for an AGG genotype is a ratio selected from the group consisting of (k the discriminatory variable value for a GGG genotype is a ratio of k