Patent ID: 6110696
Filing Date: 2000-08-29
Classification: C12Q,G01N,Y10S

Abstract:
A diagnostic kit for determining the presence or concentration of an analyte in a fluid sample, comprising:(a) an enzyme donor reagent which comprises1) an enzyme donor polypeptide conjugate; and2) a labeled substrate, comprising an enzyme substrate cleavably linked to an electroactive label;(b) an enzyme acceptor reagent which comprises1) an enzyme acceptor polypeptide capable of combining with the enzyme donor polypeptide conjugate to form an active enzyme complex capable of catalyzing the cleavage of the electroactive label from the enzyme substrate; and2) a first antibody capable of immunologically, competitively binding to the analyte and the enzyme donor polypeptide conjugate and hindering formation of the active enzyme complex when bound to the enzyme donor polypeptide conjugate; and(c) an electrochemical immunosensor.