Patent ID: 7662973
Filing Date: 2010-02-16
Classification: C09B,G01N

Abstract:
1. A method for increasing the fluorescence of a cyanine dye molecule which includes at least one NO 2 group, comprising reducing said at least one NO 2 group of said cyanine dye to NHOH or NH 2 in a nitroreductase reporter gene assay; wherein the cyanine dye molecule is of Formula Ia, Formula Ib or Formula Ic: wherein groups R Z X and Y independently represent a carbon atom substituted with two C1-C4 alkyl residues that may be linked to form a C4-C5 ring system or are selected from the group consisting of oxygen, sulphur, selenium, —CH═CH— and N—W wherein N is nitrogen and W is selected from hydrogen, a group —(CH groups R groups R and further wherein at least one of the groups R