Patent ID: 6569656
Filing Date: 2003-05-27
Classification: C12N

Abstract:
An isolated synthase having a region with 40% or greater sequence identity to residues 265 to 535 of SEQ ID NO:2, wherein one or more amino acid residues of said synthase that align with amino acids at positions 270, 273, 294, 297, 298, 376, 401, 402, 403, 404, 407, 440, 444, 516, 519, 520, 525, 527, and 528 of SEQ ID NO:2 are residues other than the following ordered arrangements of residues:Ordered Arrangement of R-Groups at &agr;-carbons 1-1912345678910CWIISYTTTYCWIISYTSTYGWIASYTCGYGWIASYTSGYCWLTSYSAGYGWLLSYSTVHCWLTSYSAGYLWITTYSVGNPWIVDYSTAGAWVCGFTSCINFFLGAEITACWNITYSISGSWVLTYSSSYNFFLVNATLACWNITYISGPCWNVTYIGGICYLLTFAVTMCWIITYSISASWFIVFSSSVSWIATYSVASNWNLTYSISSFLAQTYSIGQISSTVYSIALYLCITYSCGHGSFITFSSSVYWACTYSSGMAANLTNALTSFLCVTYSSAYFWAMTYNTGMYMCVTFVSSGVSGQVYSVGLCSGTTMFALGCSGTTMSFALCAGTTMSFALIWVISYTTGLYWACTYSSGMCWIISYTSTYCWIISYTTTYCWNITYSISGFAAQTYSIGQFAIATYSVAS111213141516171819LCDVTYDYTLCDITYDYTLCDMLYDYTLCDMLYDYTIANALYDYTLGDAVYDYTIANALYDYSLFDVLYDFTLSDACYDYTMGNCSYDYSTGNITYEFTMLDAMYDHQLGGVLYDFTLGNLSYEFTLLDAMYDHGLLDAIYDFGTGNITYDYTILDAIYDDGILNVIYDHGILDAIYDFGIFNSMYDHGLSDTIFDFGVGNMFYDLTSLGFGYDYSILNAVYDHGLGDLIYDLYTCMLLYDYNVLGLLYDFSLSDIMYDFSILGFVYDYTCWNVFYDYGVGNLFYDFTIGNLFYDFTIGNVFYDYTVINTSYDYTLGDLIYDLYLCDVTYDYTLCDITYDYTMLDAMYDHGLSDTIFDFGILDAIYDFâ€‰G.