Patent ID: 7390641
Filing Date: 2008-06-24
Classification: C12N

Abstract:
1. A purified peroxidase enzyme from Azadirachta indica (neem) or Vitex negundo (nirgundi), said enzyme having the following characteristics: Natural Source: i. Location: Extra-cellular; ii. Mol. wt: 36 kD; iii. Optimum pH: about 4.5; iv. Stability: >30% activity retained after 1 year at 4° C. in sodium acetate or phosphate buffer; v. Haem Peak: λ maximum about 403 nm; vi. Reinheitszah (RZ) value: about 2.05; vii. Isoelectric point (pI): >10; viii. Specific Activity: about 12046 to about 94650 U/mg (ABTS); and ix. Glycosylation: non-glycosylated, or Natural Source: i. Location: Extra-cellular as well as intracellular; ii. Mol. wt: 34 kD; iii. pH range: 3.5-8.5; iv. Optimum pH: about 4.5; v. Optimum temperature: about 50° C.; vi. Stability: >80% activity retained even after keeping the crude extract as well as cell suspension supernatant for a period of 6 months at 4° C. in sodium acetate or phosphate buffer; vii. Haem Peak: λ maximum about 409 nm; and viii. Reinheitszah (RZ) value: about 2.10.