Patent ID: 6534290
Filing Date: 2003-03-18
Classification: A61K,A61P,C07K

Abstract:
A method for producing a protein having the following characteristics:(i) an estimated molecular weight of about 45 kDa, said estimate obtained by electrophoresing the HPLC-purified polypeptide on an SDS-polyacrylamide gel; (ii) capability of stimulating mitogenic activity when in contact with cultured cells; and (iii) released by BSC-1 African green monkey kidney epithelial cells in culture by scrape-wounding; said method comprising: (a) culturing the kidney epithelial cells in media; (b) wounding cells in culture; and (c) obtaining the protein from the conditioned media; wherein the kidney epithelial cells in culture are from the BSC-1 African green monkey kidney epithelial cell line.