Patent ID: 6680182
Filing Date: 2004-01-20
Classification: A61K,C07K,C12N,Y02A

Abstract:
An attenuated bacterium comprising a DNA construct comprising a promoter operably linked to a DNA sequence encoding a first and second immunogeiic polypepies and a hinge region,wherein the attenuated bacterium is viable under anaerobic conditions, wherein the promoter induces expression of operably linked DNA sequences under anaerobic conditions, wherein the DNA sequence encoding the first immunogenic polypeptide and the DNA sequence encoding the second immunogenic polypcptide are linked by a DNA sequence encoding a chain of amino acids defining a hinge region, wherein the first immunogenic polypeptide comprises a tetanus toxin C fragment or an epitope thereof, wherein the second immunogenic polypeptide comprises an immunogenic polypeptide of a pathogenic organism, and wherein the DNA construct provides an enhanced level of expression of said second immunogenic polypeptide in said attenuated bacterium relative to a DNA construct, wherein the DNA sequence encoding the first immunogenic polypeptide and said hinge region are absent.