Patent ID: 7390651
Filing Date: 2008-06-24
Classification: C12N,C12P

Abstract:
1. An extracorporeal bio-artificial liver device comprising an apparatus containing cells of the cell line deposited as ATCC accession No. CRL-12461, wherein the cells have a doubling time in serum-free medium which is less than about 70% of the doubling time in serum-free medium for C3A cells, wherein the cells are cultured in serum-free medium on a surface in the device and express one or more proteins selected from the group consisting of albumin, alpha-1-antitrypsin, factor V, complement C3, antithrombin III, and transferrin, and wherein the surface is contained within a hollow fiber cartridge, wherein the hollow fiber is formed from a membrane which has a pore size of about 0.1 μm to 0.3 μm, and wherein the cartridge is at least 1400 cm 2 .