Patent ID: 7927789
Filing Date: 2011-04-19
Classification: G01N,Y10S

Abstract:
1. A method of making a self-referencing colorimetric resonant optical biosensor comprising: (a) immobilizing one or more specific binding substances in an immobilization volume to one or more assay regions of a self-referencing colorimetric resonant optical biosensor, wherein the biosensor comprises a liquid holding vessel comprising a colorimetric resonant optical biosensor as a surface, and a fluid-impermeable divider on the colorimetric resonant optical biosensor surface, wherein the fluid impermeable divider separates the liquid holding vessel into two or more assay regions, wherein the fluid-impermeable divider segregates an immobilization volume within each assay region, and wherein the fluid-impermeable divider allows a reaction volume to encompass all assay regions in the liquid-holding vessel; and (b) preserving one or more assay regions as one or more reference regions, wherein the one or more reference regions do not contain immobilized specific binding substances.