Patent ID: 6413942
Filing Date: 2002-07-02
Classification: A61K,C07K,C12N,C12Y

Abstract:
A method for delivering a physiologically active polypeptide to a mammal, comprising:administering in vivo into a tissue of a mammal a composition comprising a polynucleotide which directs synthesis of said polypeptide in mammalian cells, and a pharmaceutically acceptable carrier; wherein said tissue is selected from the group consisting of muscle and skin; wherein said polynucleotide is a DNA plasmid operably encoding said physiologically active polypeptide through association with a promoter; wherein said polynucleotide is free from association with liposomal formulations and charged lipids; wherein a sufficient amount of said composition is administered to allow incorporation of said polynucleotide into the cells of said mammal; and wherein said polypeptide is expressed.