Patent ID: 7547523
Filing Date: 2009-06-16
Classification: C07K,G01N

Abstract:
1. A method for identifying an agonist or antagonist of a mammalian histamine receptor, comprising: (a) contacting a polypeptide comprising the amino acid sequence defined by SEQ ID NO: 2 or a histamine binding fragment thereof in the presence of a known amount of labeled histamine or a surrogate thereof with a sample to be tested for the presence of a histamine agonist or antagonist; (b) measuring the amount of labeled histamine or surrogate thereof specifically bound to the polypeptide; (c) contacting cells expressing a polypeptide comprising the amino acid sequence defined by SEQ ID NO: 2 or a histamine binding fragment thereof with said sample; and (d) measuring at least one cellular function modulated by the binding of a ligand to a polypeptide comprising the amino acid sequence defined by SEQ ID NO: 2; whereby a histamine agonist in the sample is identified by measuring: and whereby a histamine antagonist in the sample is identified by measuring: