Patent ID: 6406868
Filing Date: 2002-06-18
Classification: C07K

Abstract:
A method of assaying a test ligand for the ability to bind to a human CNS receptor, said method comprising the steps of:(a) incubating said test ligand with: (i) a genetically engineered cell that produces an AMPA-bindinq human receptor GluR1B having the amino acid sequence of residues 1-888 of SEQ ID NO:2, said cell having incorporated expressibly therein a heterologous DNA molecule encoding the human GluR1B said cell further comprising a membrane, wherein said membrane comprises said human GluR1B, or (ii) a membrane preparation comprising human GluR1B derived from said cell, wherein said incubation is performed under conditions which permit binding of said test ligand with said human GluR1B; and (b) determining the extent of binding between said test ligand and said human GluR1B.