Patent ID: 7244562
Filing Date: 2007-07-17
Classification: C12Q

Abstract:
1. A method of detecting a single nucleotide polymorphism (SNP) in a double stranded target DNA molecule, comprising: (a) providing the following single stranded DNA oligonucleotide probes: (b) contacting the first and the second probes, with RecA protein to form RecA filaments; (c) contacting the RecA filaments with the target DNA, thereby forming a three stranded DNA D-loop structure in the target DNA, which D-loop structures comprise the probes and the two strands of the target DNA; (d) contacting the DNA D-loop structure with a DNA ligase, thereby joining the probes to form a ligation product; (e) detecting the presence of said ligation product, the presence of which is indicative of the presence of said SNP.