Patent ID: 6245512
Filing Date: 2001-06-12
Classification: A61K,C07K,C12N,C12Q

Abstract:
An assay for screening for a test compound that regulates the activity of a VEGF receptor promoter comprising:(a) contacting one or more isolated host cells, wherein said host cells are transformed with an expression vector comprising an isolated nucleic acid molecule which comprises a VEGF receptor promoter region, wherein said promoter region is the promoter contained in SEQ ID NO: 1 and said promoter being operably linked to a reporter gene encoding a gene product, with a test medium containing said test compound under conditions which allow for expression of said reporter gene;(b) measuring the expression of the reporter gene of (a) in the presence of said test medium;(c) contacting one or more isolated host cells, wherein said host cells are transformed with an expression vector comprising an isolated nucleic acid molecule which comprises a VEGF receptor promoter region, said promoter region being contained in SEQ ID NO: 1 and said promoter being operably linked to a reporter gene encoding a gene product, with a control medium which does not contain said test compound but is otherwise identical to said test medium in (a), under conditions identical to those used in (a);(d) measuring the expression of the reporter gene of (c) in the presence of said control medium; and(e) wherein the difference in expression between (b) and (d) indicates the test compound regulates the activity of the VEGF receptor promoter.