Patent ID: 6635420
Filing Date: 2003-10-21
Classification: B01D,C07K,Y10S

Abstract:
A method for purifying a target substance from a sample comprising the steps of:(a) providing a solid phase with an immobilized reactant R1 wherein R1 comprises at least one group that binds specifically to said target and at least one group comprising a first partner of a binding pair, wherein the binding pair is characterized by having an affinity constant from about 106 l/mol to 1010 l/mol, and wherein binding of R1 to said solid phase occurs by means of an immobilized reactant R2 which comprises a group comprising a second partner of said binding pair, (b) contacting said sample with said solid phase resulting in adsorption of said target onto said solid phase via binding to R1, (c) separating non-adsorbed sample components from said solid phase, (d) contacting said solid phase with a first partner of said binding pair or analog thereof in free form resulting in desorption of said target as a complex with R1 from said solid phase, and (e) separating said complex from said solid phase.