Patent ID: 8952213
Filing Date: 2015-02-10
Classification: A01K,C12N,C12Q

Abstract:
1. A method of identifying one or more living sensory neurons activated by a stimulus in a transgenic mouse, said method comprising: (a) providing a transgenic mouse whose genome comprises a nucleic acid construct comprising the c-fos 5′ untranslated region, which comprises one or more regulatory sequences and the complete genomic sequence of the c-fos gene fused in frame to a detectable reporter, wherein the one or more regulatory sequences is activated in a sensory neuron in response to a predetermined chemical, behavioral, or pharmacological stimulus, (b) administering the predetermined chemical, behavioral, or pharmacological stimulus to the transgenic mouse of step (a), (c) identifying ex vivo in a brain slice derived from the transgenic mouse in step (b) one or more living sensory neurons expressing the detectable reporter, while maintaining the sensory neuron's viability, and wherein the detectable reporter is a luminescent or fluorescent protein.