Patent ID: 8980179
Filing Date: 2015-03-17
Classification: G01N,Y10S

Abstract:
1. A method for detecting emissions from fluorescent molecules positioned near metallic structures, the method comprising: (a) positioning the fluorescent molecules near the metallic structures immobilized on a surface, wherein the surface comprises a multiplicity of metallic structures and wherein the fluorescent molecules are positioned from about 5 nm to about 30 nm from the metallic structures; (b) irradiating the fluorescent molecules with polarized radiation and at an excitation incidence angle in an amount sufficient to cause non-radiative transfer of energy from the fluorescent molecules to plasmons on the metallic structures; and (c) detecting angular plasmonic emissions from the metallic structures in combination with scattered emissions from the fluorescent molecules, at a detection angle different from that of the excitation incidence angle and wherein the detection angle is about 180 degrees relative to the angle of incidence.