Patent ID: 8110383
Filing Date: 2012-02-07
Classification: C12P,Y02E

Abstract:
1. A method for preparing at least one target chemical obtained from cellulosic biomass involving detoxification of spent hydrolysate, comprising the steps of: a) providing cellulosic biomass; b) subjecting the cellulosic biomass to at least one aqueous pretreatment fluid to provide pretreated cellulosic biomass; c) subjecting the pretreated cellulosic biomass to at least one aqueous hydrolysing liquid, optionally comprising saccharification enzymes, under conditions in which at least a part of the pretreated cellulosic biomass is hydrolysed to a cellulosic hydrolysate, said cellulosic hydrolysate comprising fermentable sugars and inhibitory substances; d) subjecting the fermentable sugars to fermentation in an aqueous liquid utilizing at least one fermentation biocatalyst under conditions in which at least a part of the fermentable sugars are fermented into a primary target chemical; e) separating the primary target chemical from the fermented hydrolysate to provide a spent hydrolysate comprising inhibitory substances; f) detoxifying the spent hydrolysate by decreasing the concentration of at least one of the inhibitory substances using a detoxification biocatalyst selected from the group consisting of wild type, mutant and recombinant filamentous fungi, to provide a detoxified spent hydrolysate; g) recirculating at least a part of the detoxified spent hydrolysate, optionally after further purification, as a part of aqueous liquid(s) provided in at least one of steps b), c) and d).