Patent ID: 8425770
Filing Date: 2013-04-23
Classification: B01D,G01N

Abstract:
1. An apparatus for isolating a target molecule, comprising: at least one rotating chamber with an inlet port and an outlet port, the chamber containing a plurality of chromatography particles in an interior thereof and rotating about a horizontal axis to create a centrifugal force on the particles; a first pressurized liquid source containing a first liquid, the first pressurized liquid source in fluid communication with the inlet port and configured to flow the first liquid through the inlet port, through the chamber, and out of the outlet port, such that a liquid flow force is imparted on the chromatography particles inside the chamber, wherein the liquid flow force substantially opposes the centrifugal force, wherein a gravitational force contributes to a resultant vector summation of all forces acting on the chromatography particles, and wherein the gravitational, liquid flow, and centrifugal forces substantially immobilize the chromatography particles in a fluidized chromatography bed inside the chamber; and a second pressurized liquid source containing a heterogeneous liquid comprising the target molecule, the second pressurized liquid source in fluid communication with the inlet port and configured to flow the heterogeneous liquid through the inlet port and to the fluidized chromatography bed; wherein the outlet port is configured to discharge the heterogeneous liquid less the target molecule.