Patent ID: 6638732
Filing Date: 2003-10-28
Classification: C07K

Abstract:
A nucleic acid molecule encoding a mutant Green Fluorescent Protein (GFP), mutated at amino acid positions corresponding the amino acid positions 64 and 65 of SEQ ID NO:4 by substitution mutations that are selected from the group consisting of:(a) a cysteine residue substituted for the phenylalanine residue at the amino acid position corresponding to amino acid position 64 of SEQ ID NO:4, and an alanine residue substituted for the serine residue at the amino acid position corresponding to amino acid position 65 of SEQ ID NO:4; and (b) a methionine residue substituted for the phenylalanine residue at the amino acid position corresponding to amino acid position 64 of SEQ ID NO:4, and an alanine residue substituted for the serine residue at the amino acid position corresponding to amino acid position 65 of SEQ ID NO:4.