Patent ID: 8652850
Filing Date: 2014-02-18
Classification: C09B,G01N,Y10T

Abstract:
1. A method of quantitatively determining 8-isoprostane, the method comprising: fluorescently labeling 8-isoprostane with a quinoxalinone derivative in an excess amount relative to amount by mole of 8-isoprostane, the quinoxalinone derivative which is represented by Formula (1) below wherein, in Formula (1), R contacting a reaction mixture containing a fluorescently labeled 8-isoprostane and an unreacted quinoxalinone derivative with a cation exchange support having a sulfonic acid or a sulfonate immobilized thereon to separate the fluorescently labeled 8-isoprostane from the unreacted quinoxalinone derivative, and quantitatively determining the fluorescently labeled 8-isoprostane separated from the unreacted quinoxalinone derivative.