Patent ID: 7598093
Filing Date: 2009-10-06
Classification: B82Y,G01N,Y10T

Abstract:
1. A method of detecting a secreted cell product, comprising: a) providing a reaction chamber comprising: b) adding a sample of cells to said reaction chamber and permitting at least one of said cells to adhere to said substrate; c) stimulating said cells to produce said product, wherein said product binds to said capture reagent to form a product-capture reagent complex at a location near said cell on said substrate and then washing away said cells to expose said location; d) adding a detection reagent to said reaction chamber, wherein said detection reagent binds to said product producing a detection reagent-cell product-capture reagent complex (DRC); e) adding a detection particle to said chamber, wherein said detection particle binds to said detection reagent via streptavidin/biotin binding producing a detection particle-detection reagent-cell product-capture reagent complex (DRCC); and f) visualizing said detection particle on said DRCC as a spot on said substrate.