Patent ID: 6150172
Filing Date: 2000-11-21
Classification: C07K,Y10S

Abstract:
A method for extracting abnormal prion protein from a biological material suspected of containing abnormal prion protein, said method comprising:(a) incubating a mixture of an extraction solvent and an isotonic or hypotonic aqueous preparation of biological material under conditions effective to extract abnormal prion protein from said biological material into said extraction solvent, wherein said extraction solvent is;(i) a polar organic solvent in which said abnormal prion protein is soluble, and(ii) miscible with a non-lyotropic aqueous solution but immiscible with a lyotropic aqueous solution; and(b) increasing lyotropic activity of the mixture to separate said extraction solvent from said aqueous preparation of said biological material to yield extraction solvent containing any abnormal prion protein from said biological material.