Patent ID: 7977043
Filing Date: 2011-07-12
Classification: C12Q,G01N

Abstract:
1. A method for determining if a test compound inhibits the production of ADP resulting from the reaction of CD38 with NAD + to produce ADPR, subsequent reaction of the ADPR with ADPRase to produce AMP and subsequent reaction of the AMP with myokinase and ATP to produce ADP, said method comprising: (a) forming a first reaction mixture consisting essentially of NAD (b) incubating both the first reaction mixture and the second reaction mixture; (c) adding a luciferin/luciferase reagent to generate luminescence by reacting with any residual ATP to both the first reaction mixture and the second reaction mixture; (d) measuring the luminescence resulting from step (c) in both the first reaction mixture and the second reaction mixture; and (e) comparing the luminescence generated in step (c) between the first reaction mixture and the second reaction mixture wherein more luminescence in the first reaction mixture as compared to the second reaction mixture indicates that the test compound inhibits production of ADP.