Patent ID: 7985575
Filing Date: 2011-07-26
Classification: C12N

Abstract:
1. A system for expressing a single target protein in a transformable living cell while reducing non-target cellular protein synthesis, comprising (a) an isolated transformable living cell comprising cellular mRNA having at least one first MazF recognition sequence; (b) a first expression vector comprising an isolated nucleic acid sequence encoding MazF polypeptide, wherein the isolated nucleic acid sequence encoding the MazF polypeptide is mutated by replacing at least one second MazF recognition sequence with an alternate triplet codon sequence to produce a mutated nucleic acid sequence encoding an amino acid sequence identical to the amino acid sequence of a nonmutated MazF polypeptide; (c) optionally, a second expression vector comprising an isolated nucleic acid sequence encoding a target protein, wherein the isolated nucleic acid sequence encoding the target protein is mutated by replacing at least one third MazF recognition sequence with an alternate triplet codon sequence to produce a mutated nucleic acid sequence encoding an amino acid sequence identical to the amino acid sequence of a nonmutated target protein; wherein the isolated cell is transformed with the first expression vector and the second expression vector; and wherein the isolated cell is maintained under conditions permitting expression of the mutant target protein in the cell.