Patent ID: 6664110
Filing Date: 2003-12-16
Classification: G01N,Y10T

Abstract:
Reagents for discriminating and counting erythroblasts in body fluid samples by flow cytometry, consisting essentially of one fluorescent dye selected to stain leukocytes strongly and crythroblasts weakly in the body fluid samples to produce a flow-cytometric detectable intense fluorescence in the leukocytes relative to the erytlroblasts, the one fluorescent dye being selected from the group consisting of the following: wherein R1, R2 are either a hydrogen meolecule, an alkyl group, an alkynyl group or an alkyl group substituted with a hydroxyl, Y, Z are either sulfur, oxygen, nitrogen or carbon having a lower alkyl groups, n is 0, 1 or 2, and Xâˆ’ is an anion; wherein R1 is a hydrogen molecule or a dimethylamino group, R2 is an alkyl group, R3 is a hydrogen molecule or a dimethylamino group, n is 1 or 2, and Xâˆ’ is an anion; wherein R1 is a hydrogen molecule or an alkyl group, R2 is a dimethylamino group, R3 is a hydrogen molecule or an amino group, R4 is a hydrogen molecule, an alkyl group or an amino group, R5 is a hydrogen molecule or a dimethylamino group, Xâˆ’ is an anion, and Y is sulfur or oxygen; wherein R1 is a hydrogen molecule or a hydroxyl, R2, R3 are a hydrogen molecule or a sulfonic group, and Y+ is an alkali metal ion; anda hemolytic agent containing intra-molecularly at least one of an organic acid having at least one aromatic ring, and a salt thereof, in an aqueous solution having a pH of approximately 2.0-5.0 and an osmotic pressure of approximately 100 mOsm/kg or less, for dissolving erythrocytes in a body fluid sample to an extent such that said hemolytic agent does not interfere with discrimination and counting by flow cytometry of erythroblasts in the body fluid sample, and such that said hemolytic agent conditions leukocytes and erythroblasts in the body fluid sample to be suitable for staining; wherein said reagent has a pH of approximately 2.0 to 5.0.