Patent ID: 6821759
Filing Date: 2004-11-23
Classification: A01K,C07K,C12N

Abstract:
A method of selectively performing homologous recombination with a particular nucleotide sequence of a Bacterial or Bacteriophage-Derived Artificial Chromosome (BBPAC) that is contained in a recombination deficient host cell comprising introducing a conditional replication shuttle vector into a recombination deficient host cell and therein enabling homologous recombination in the host cell via the transient expression of a recombination protein in the host cell;wherein the host cell comprises a Bacterial or Bacteriophage-Derived Artificial Chromosome (BBPAC) which contains the particular nucleotide sequence; wherein the conditional replication shuttle vector encodes a recombination protein that is transiently expressed by the host cell; wherein the conditional replication shuttle vector contains homologous nucleic acid sequences capable of selectively integrating into the particular nucleotide sequence when the recombination protein is expressed; and wherein the expressed recombination protein effectuates recombination of the shuttle vector and the Bacterial or Bacteriophage-Derived Artificial Chromosome (BBPAC).