Patent ID: 8663917
Filing Date: 2014-03-04
Classification: C12Q

Abstract:
1. A process for obtaining genomic copy number information about genomic DNA from a diseased cell of an organism, which does not involve Representational Difference Analysis (RDA), comprising a) providing a representation of the genomic DNA by digesting the genomic DNA from the diseased cell with a single restriction endonuclease to provide digested DNA fragments, ligating an adaptor to the digested DNA fragments to form ligated DNA fragments, and polymerase chain reaction (PCR) amplifying the ligated DNA fragments using a single set of primers complementary to the adaptor to provide the representation of the genomic DNA; b) contacting nucleic acids from the representation provided in part (a), and a second representation obtained from genomic DNA from a normal cell from the same organism, with a microarray comprising c) detecting the hybridization at addresses of defined coordinates of the microarray in step b), d) obtaining genomic copy number information based on the hybridization detected in step c).