Patent ID: 9023651
Filing Date: 2015-05-05
Classification: B82Y,G01N,G01R

Abstract:
1. A method for determining the amount of troponin in a sample over a range from 60 to 100,000 pM, wherein magnetic particles specifically bind to a contact surface of a sample chamber filled with the sample with kinetics that depend on the sample-amount of the troponin, the method comprising the following steps: a) a first washing step in which a magnetic force is exerted on the magnetic particles such that the unbound magnetic particles are moved away from the contact surface; b) a first measurement step in which the total amount of magnetic particles at the contact surface is measured during the first washing step; c) an attracting step for pulling magnetic particles to the contact surface; d) a second washing step in which a magnetic force is exerted on the magnetic particles such that the unbound magnetic particles are moved away from the contact surface; e) a second measurement in which the total amount of magnetic particles at the contact surface is measured during the second washing step; f) estimating the sample-amount of troponin by correlating the result of the first or second measurement with known sample amounts of troponin when the total amount of troponin is below a threshold; and g) estimating the sample-amount of troponin by correlating the change between the results of the first and second measurements with known changes in sample amounts of troponin when the total amount of troponin is above a threshold; wherein the threshold is 1000 pM.