Patent ID: 6403302
Filing Date: 2002-06-11
Classification: C12N,C12Q

Abstract:
A triple-helix comprising a double-helical nucleic acid comprising first and second substantially complementary strands and an oligonucleotide bound to a target sequence within said double-helical nucleic acid, wherein said first strand comprises a first purine-rich sequence and a pyrimidine-rich sequence and said target sequence comprises said first purine-rich target sequence and a second purine-rich target sequence on said second strand based paired with said pyrimidine-rich sequence, and wherein said oligonucleotide has 5â€² and 3â€² terminal nucleotides and comprises a first binding domain comprising a pyrimidine-rich portion bound to said first purine-rich target sequence in a parallel orientation and a second binding domain comprising a purine-rich portion bound to said second purine-rich target sequence in an antiparallel orientation and a linking domain between said first and said second binding domains having a length which permits each of the said binding domains to bind to purine-rich target sequences.