Patent ID: 8563305
Filing Date: 2013-10-22
Classification: C07K

Abstract:
1. A method for producing an antibody molecule that binds an antigen, the method comprising: (a) obtaining a sample of cells enriched for PBMC from a biological sample comprising antibody producing cells obtained from a mammal after exposure of the mammal to the antigen; (b) isolating a population of CD19 (c) isolating from a selected CD19 (d) transforming a recombinant cell with the nucleic acid sequence encoding at least a portion of the antibody light chain comprising the light chain variable domain and a nucleic acid sequence encoding at least a portion of the antibody heavy chain comprising the heavy chain variable domain, wherein the light chain variable domain and the heavy chain variable were paired in the selected cell; and (e) culturing the recombinant cell to produce an antibody molecule the binds the antigen.