Patent ID: 6054264
Filing Date: 2000-04-25
Classification: C07K,C12N,G01N,Y10S

Abstract:
A method for detecting one or more types of a hepatitis C virus comprising the steps of:(a) providing a biological sample from an individual suspected of being infected by a hepatitis C virus,(b) contacting the sample with a composition comprising a first reagent said first reagent consisting essentially of a combination of hepatitis C virus type specific epitopes specific for a first type of hepatitis C virus, wherein said epitopes are from the core, NS4 or NS5 regions of the hepaitis C virus and at least one epitopes is derived from the amino acid sequence spanning amino acids 1689 to 1718 of hepatitis C-virus-1 or a homologous region thereof of other hepatitis C virus types, and wherein said contacting is carried out under conditions which allow the formation of first epitope-antibody complexes; and(c) assaying for the presence of said first epitope-antibody complexes in the sample, wherein said assaying performed by a competition assay, a sandwich assay, an immunofluoresence assay, a chemilumenscence assay (CLIA), a radio-immnuoassay, or an enzyme-linked immunosorbent assay.