Patent ID: 6689562
Filing Date: 2004-02-10
Classification: A01K,A61K,A61P,C07K,C12Q,G01N

Abstract:
A method of identifying a compound that modulates IAP expression levels, said method comprising:(a) providing a cell expressing a native mammalian IAP gene encoding a polypeptide having at least 95% sequence identity to XIAP (SEQ ID NO: 4), HIAP1 (SEQ ID NO: 6), HIAP2 (SEQ ID NO: 8), m-XIAP (SEQ ID NO: 10), or m-HIAP1 (SEQ ID NO: 40), said polypeptide comprising a domain having a sequence selected from the group consisting of amino acids 26-93, 163-230, or 265-330 of SEQ ID NO: 4, amino acids 26-93, 163-230, or 264-329 or SEQ ID NO: 10, amino acids 29-96, 169-235, or 255-322 of SEQ ID NO: 6, amino acids 29-96, 169-235, or 255-322 of SEQ ID NO: 40, or amino acids 46-113, 184-250, or 269-336 of SEQ ID NO: 8; (b) contacting said cell with a candidate compound; and (c) determining the expression levels of said gene, an alteration in the level of said expression levels, relative to said levels of said gene in the absence of said candidate compound, indicating the presence of a compound that modulates IAP expression levels, wherein said method is carried out ex vivo.