Patent ID: 6338846
Filing Date: 2002-01-15
Classification: A01N,C07K,C12N

Abstract:
A recombinant baculovirus comprising a genome wherein the genome comprises two independent transcription units:wherein the first transcription unit comprises a nucleic acid sequence encoding a functional polyhedrin protein capable of forming a paracrystalline lattice around the viral particle operably linked to promoter; and, wherein the second transcription unit transcribes a fusion gene wherein the fusion gene comprises a nucleic acid sequence encoding a polyhedrin, a gfp nucleic acid sequence encoding a green fluorescent protein of a jellyfish Aequorea victoria and nucleic acid sequence encoding a Bacillus thuringiensis crystal protein, or fragment thereof, having insecticidal activity and in which the nucleic acid sequences are sequentially linked and are operably linked to a polyhedrin promoter so that infectious occlusion bodies with the viral particle produced from said baculovirus contains a fusion gene product, wherein the fusion gene product encodes a fusion protein wherein the baculovirus polyhedron protein, Bacillus thuringiensis crystal protein and jellyfish Aequorea victoria green fluorescent protein are sequentially linked from N-terminal to C-terminal.