Patent ID: 6504082
Filing Date: 2003-01-07
Classification: C07K,C12N

Abstract:
A method of selectively inducing gene expression of a nucleotide sequence of interest in a plant, said method comprising:a) stably incorporating into the genome of said plant a first expression cassette, said first expression cassette comprising a first promoter operably linked to a first nucleotide sequence that encodes a first protein and is selected from the group consisting of: i) a nucleotide sequence comprising the sequence set forth in SEQ ID NO: 1; ii) a nucleotide sequence comprising nucleotides 368-2005 of SEQ ID NO:1; and, iii) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:2; b) stably incorporating into the genome of said plant a second expression cassette, wherein said second expression cassette comprises a transcriptional regulatory region operably linked to a target sequence; c) stably incorporating into the genome of said plant a third expression cassette comprising a promoter operably linked to a third nucleotide sequence that encodes a second protein and is selected from the group consisting of: 1) a nucleotide sequence comprising the sequence set forth in SEQ ID NO: 3; 2) a nucleotide sequence comprising nucleotides 224-1603 of SEQ ID NO:3; and 3) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 4; and d) contacting said plant with a ligand that binds to at least one of said proteins to form a ligand-protein complex, wherein said ligand-protein complex interacts with said transcriptional regulatory region to induce gene expression of said target sequence.