Patent ID: 6962784
Filing Date: 2005-11-08
Classification: A61K,C07H,C07K,C12N

Abstract:
1. A method for the preparation of a Complex comprised of a VEGF Nucleic Acid Ligand comprising 2′-F-modified nucleotides and a Non-Immunogenic, High Molecular Weight Compound or Lipophilic Compound, said method comprising: a) identifying a VEGF Nucleic Acid Ligand from a Candidate Mixture of Nucleic Acids by the method comprising: i) contacting the Candidate Mixture with VEGF, wherein Nucleic Acids having an increased affinity to VEGF relative to the Candidate Mixture may be partitioned from the remainder of the Candidate Mixture; ii) partitioning the increased affinity VEGF Nucleic Acids from the remainder of the Candidate Mixture; iii) amplifying the increased affinity VEGF Nucleic Acids to yield a ligand-enriched mixture of Nucleic Acids; and b) associating said identified VEGF Nucleic Acid Ligand with a Non-Immunogenic, High Molecular Weight Compound or Lipophilic Compound.