Patent ID: 6352723
Filing Date: 2002-03-05
Classification: A61K,A61P,C07K

Abstract:
A mammalian brain homogenate containing an alkali soluble active factor which inhibits antigen-stimulated proliferation of lymphocytes in vitro, wherein said active factor sediments in a particulate complex at 14,000 g; is heat stable; is inactivated by digestion with trypsin, proteinase K or neuraminidase; is not inactivated by formalin treatment or digestion with hyaluronidase; and, can be partially purified by a method comprising the following steps:a) centrifuging the brain homogenate at 14,000 g, b) solubilizing the factor in 0.04 N NaOH, c) applying the resulting solubilized factor to a 6HR 10/30 sepharose gel filtration column equilibrated with 0.04 N NaOH, and d) eluting said factor from the column with 0.4 N NaOH.