Patent ID: 6686168
Filing Date: 2004-02-03
Classification: C07H,C12N,C40B

Abstract:
A method of selecting nucleic acid molecules that encode a member of a complementary/anti-complementary binding pair, comprising:(a) transfecting an expression vector into eukaryotic host cells to produce recombinant host cells, wherein the expression vector comprises: (i) a eukaryotic promoter, (ii) a nucleotide sequence encoding a type II signal anchor domain segment, and (iii) a gene, or gene fragment, that encodes the first member of a complementary/anti-complementary binding pair, wherein the expression vector comprises elements (i) to (iii) in a 5â€² to 3â€² order, wherein the gene, or gene fragment, resides in-frame with the nucleotide sequence that encodes the type II signal anchor domain, and wherein the nucleotide sequence that encodes the type II signal anchor domain segment is heterologous with respect to the gene, or gene fragment, (b) incubating the recombinant host cells under conditions and a time sufficient for expression of the first member of a complementary/anti-complementary binding pair on the surface of the recombinant cells, (c) exposing the recombinant cells to the second member of the complementary/anti-complementary binding pair, and (d) selecting recombinant cells that bind the second member of the complementary/anti-complementary binding pair.