Patent ID: 6043066
Filing Date: 2000-03-28
Classification: C12M,C12N

Abstract:
A method for creating from a biological sample having a given cell population of at least a first and a second cell type, wherein said first and second cell types have a difference in at least one property affecting a characteristic electroporation threshold, a suspension of cells containing a selected viable subpopulation of said given cell population, wherein said subpopulation is enriched in said second cell type by:a. subjecting the sample to electric field conditions sufficient to porate a substantial fraction of cells of said first cell type while maintaining substantially viable cells of said second cell type, andb. selectively inactivating at least 90% of the porated cells of said first cell type in the sample subjected to the electric field conditions in step (a), while maintaining substantially viable cells of said second cell type in the sample subjected to the electric field conditions in step (a).