Patent ID: 6897358
Filing Date: 2005-05-24
Classification: A21D,A23L,C12N,C12Y

Abstract:
1. A vector containing an isolated nucleic acid molecule encoding a protein with the function of a wheat isoamylase, selected from the group consisting of (a) a nucleic acid molecule encoding a protein comprising the amino acid sequence shown under SEQ ID NO:3, (b) a nucleic acid molecule comprising the nucleotide sequence shown under SEQ ID NO:2 or a ribonucleotide sequence corresponding hereto; (c) a nucleic acid molecule which hybridizes under stringent conditions with a nucleic acid molecule mentioned under (a) or (b) or is complementary thereto, wherein hybridization comprise a hybridization temperature of 68° C., a hybridization buffer salt concentration of 5×SSC, a wash temperature of 68° C., and a wash buffer salt concentration of 0.5×SSC; and (d) a nucleic acid molecule whose nucleotide sequence deviates from the sequence of a nucleic acid molecule mentioned under (a), (b) or (c) owing to the degeneracy of the genetic code, the nucleic acid molecule mentioned under (a), (c) and (d) having sequence identity of over 90% with SEQ ID NO:2, wherein said nucleic acid molecule is linked in sense orientation to regulatory elements, which ensure the synthesis of an untranslatable RNA in prokaryotic or eukaryotic cells.