Patent ID: 7559953
Filing Date: 2009-07-14
Classification: A61F,A61L,Y10S

Abstract:
1. A method of preparing a fixed biological tissue, the method comprising: (a) harvesting at least one layer of biological tissue, the biological tissue being selected from the group consisting of pericardial tissue, peritoneal tissue, and pleural tissue, the layer of biological tissue comprising an inner lining of a serous membrane and an outer lining of associated fascia; (b) shaping the biological tissue, wherein the shaping comprises trimming and shaping the harvested layer of biological tissue to a desired size and configuration; (c) at least partially cross-linking the biological tissue by contacting the biological tissue with a solution containing at least one cross-linking agent; (d) sterilizing the biological tissue by contacting the at least partially cross-linked biological tissue with a solution containing an alcohol; and (e) inactivating prions in the sterilized biological tissue, the prions in the biological tissue being inactivated by contacting the sterilized biological tissue with a basic solution having a molar concentration of about 0.5 M to about 4.0 M; whereby steps (a)-(e) are performed sequentially.