Patent ID: 6203974
Filing Date: 2001-03-20
Classification: G01N,Y10S

Abstract:
A method for determining the presence of a Chagas Disease analyte in a test sample by specific amplification of a chemiluminescent signal generated from a heterogeneous immunoasssay comprising the steps of:a. incubating a test sample containing a Chagas Disease analyte with an analyte-specific binding pair member for a time and under conditions sufficient to form analyte/analyte specific-binding member pair complexes;b. contacting the analyte/analyte-specific binding member pair complexes with a precomplex wherein said precomplex comprises 1) a probe comprising an enhancer compound attached to an analyte-specific binding member different from said analyte-specific binding member of step (a) and 2) a conjugate comprising a chemiluminescent signal generating compound attached to an enhancer-specific binding member, and incubating said resulting mixture for a time and conditions sufficient to form analyte/analyte-specific binding member pair/precomplex complexeswherein said chemiluminescent signal generating compound is an acridinium compound or a derivative thereof;c. separating said resulting analyte/analyte-specific binding member pair/precomplex complexes of step b from free, unbound precomplexes; andd. determining the presence of said Chagas Disease analyte in said test sample by measuring the detectable signal.