Patent ID: 8354516
Filing Date: 2013-01-15
Classification: C07K,C12N

Abstract:
1. A nucleic acid comprising in a 5′ to 3′ direction a) a first nucleic acid encoding an immunoglobulin heavy chain without an in frame stop codon at its 3′ terminus , b) a second nucleic acid beginning with a 5′ splice donor site and terminated by a 3′ splice acceptor site comprising an in frame translational stop codon and a polyadenylation signal of a human or mouse immunoglobulin α, ε, γ and μ heavy chain, and c) a third nucleic acid encoding at least 60% of a complete human immunoglobulin transmembrane domain, wherein said immunoglobulin heavy chain and a marker are expressed from the same nucleic acid, wherein the marker is a plasma-membrane bound form of the expressed immunoglobulin heavy chain.