Patent ID: 9056921
Filing Date: 2015-06-16
Classification: C07K,C12N

Abstract:
1. A method for making mature human insulin analogues by culturing a fungi cell comprising a DNA vector encoding a precursor for a human insulin analogue, wherein the said precursor comprises a connecting peptide flanked with cleavage sites at both junctions to the A- and B-chain of the insulin peptide, respectively, said cleavage sites being cleaved within the fungi cell allowing the cell to secrete the correctly processed mature, two chain human insulin analogue into the culture media, wherein the insulin analogue has Glu at position A14, and a mutation in at least one of the positions A8, B10, and A18 in the human insulin A- and/or the B-chain selected from the group consisting of Asp, Glu, His, Gln and Arg.