Patent ID: 6121236
Filing Date: 2000-09-19
Classification: A61K,C07K,C12N,C12Y

Abstract:
A peptide comprising a sequence of amino acids AA.sub.1 through AA.sub.28 or a subsequence thereof having at least ten amino acids, wherein:AA.sub.1 is lysine, ornithine, arginine, N-nitroarginine, .beta.-cycloarginine, .gamma.-hydroxyarginine, N-amidinocitruline, 2-amino-4-guanidinobutanoic acid, glycine or alanine;AA.sub.2 is aspartic acid, asparagine, glutamic acid, glutamine, leucine, valine, isoleucine, methionine or a substituted or unsubstituted aliphatic or aryl ester of glutamic acid or aspartic acid;AA.sub.3 is phenylalanine, alanine, tyrosine, tryptophan, leucine, isoleucine, methionine, valine or glycine;AA.sub.4 is threonine, glycine, alanine, cysteine or serine;AA.sub.5 is alanine, threonine, glycine, cysteine or serine;AA.sub.6 is phenylalanine, tyrosine or tryptophan;AA.sub.7 is arginine, N-nitroarginine, .beta.-cycloarginine, .gamma.-hydroxyarginine, N-amidinocitruline or 2-amino-4-guanidinobutanoic acid;AA.sub.8 tryptophan, alanine, phenylalanine, tyrosine or glycine;AA.sub.9 is arginine, phenylalanine, N-nitroarginine, .beta.-cycloarginine, .gamma.-hydroxyarginine, N-amidinocitruline 2-amino-4-guanidinobutanoic acid, lysine, ornithine, tyrosine or tryptophan;AA.sub.10 is leucine, isoleucine, methionine or valine;AA.sub.11 is serine, threonine or alanine;AA.sub.12 is histidine, serine, threonine, cysteine, lysine or ornithine;AA.sub.13 is arginine, N-nitroarginine, .beta.-cycloarginine, .gamma.-hydroxyarginine, N-amidinocitruline or 2-amino-4-guanidinobutanoic acid;AA.sub.14 is proline, leucine, valine, isoleucine or methionine;AA.sub.15 is lysine, glutamine, histidine, ornithine, asparagine, arginine, N-nitroarginine, .beta.cycloarginine, .gamma.-hydroxyarginine, N-amidinocitruline or 2-amino-4-guanidinobutanoic acid;AA.sub.16 is threonine, aspartic acid, serine, glutamic acid or a substituted or unsubstituted aliphatic or aryl ester of glutamic acid or aspartic acid;AA.sub.17 is glycine, leucine, alanine, valine, isoleucine or methionine;AA.sub.18 is phenylalanine, tyrosine or tryptophan;AA.sub.19 is isoleucine, serine, valine, leucine, methionine, cysteine or threonine;AA.sub.20 is arginine, isoleucine, ornithine, lysine, N-nitroarginine, .beta.-cycloarginine, .gamma.-hydroxyarginine, N-amidinocitruline, 2-amino-4-guanidinobutanoic acid, leucine, valine or methionine;AA.sub.21 is methionine, isoleucine, leucine or valine;AA.sub.22 valine, arginine, leucine, isoleucine, methionine, ornithine, lysine, N-nitroarginine, .beta.-cycloarginine, .gamma.-hydroxyarginine, N-amidinocitruline or 2-amino-4-guanidinobutanoic acid;AA.sub.23 methionine, arginine, leucine, isoleucine, valine, ornithine, lysine, N-nitroarginine, .beta.-cycloarginine, .gamma.-hydroxyarginine, N-amidinocitruline or 2-amino-4-guanidinobutanoic acid;AA.sub.24 is phenylalanine, alanine, tyrosine, tryptophan or glycine;AA.sub.25 is aspartic acid, asparagine, glutamic acid, glutamine or a substituted or unsubstituted aliphatic or aryl ester of glutamic acid or aspartic acid;AA.sub.26 is glycine, arginine, alanine, ornithine, lysine, N-nitroarginine, .beta.-cycloarginine, .gamma.-hydroxyarginine, N-amidinocitruline or 2-amino-4-guanidinobutanoic acid;AA.sub.27 is lysine, alanine, arginine, glycine, serine, ornithine, arginine, N-nitroarginine, .beta.-cycloarginine, .gamma.-hydroxyarginine, N-amidinocitruline or 2-amino-4-guanidinobutanoic acid; andAA.sub.28 is lysine, alanine, arginine, glycine, serine, ornithine, arginine, N-nitroarginine, .beta.-cycloarginine, .gamma.-hydroxyarginine, N-amidinocitruline or 2-amino-4-guanidinobutanoic acid,wherein the peptide has angiogenic activity, with the proviso that the peptide is not represented by SEQ ID NO.: 1 or a subsequence thereof.