Patent ID: 6440707
Filing Date: 2002-08-27
Classification: C12Q

Abstract:
A method for detecting the presence of a target site, if present, of at least one nucleotide in a template nucleic acid comprising:(a) forming an oligonucleotide bound to the target site wherein the oligonucleotide comprises a fluorophore linked to a terminator contained therein; and (b) detecting fluorescence polarization of the fluorophore of the fluorescently-labeled oligonucleotide, wherein the oligonucleotide is formed from a primer bound to the template immediately 3â€² to the target site and a terminator covalently linked to a fluorophore, and wherein said terminator-linked fluorophore binds to the target site and reacts with the primer to produce an extended primer which is said fluorescently labeled oligonucleotide, wherein an increase in fluorescence polarization indicates the presence of the target site thereby detecting the presence of the target site by said increase in fluorescence polarization.