Patent ID: 6410255
Filing Date: 2002-06-25
Classification: A61P,C12Q,C40B,G01N

Abstract:
A method for measuring a post-translational modification activity, comprising:a) contacting a sample with an engineered optical probe, said engineered optical probe comprising: a polypeptide comprising a first probe moiety, wherein said polypeptide comprises a recognition motif for a post-translational type modification within said polypeptide, and a protease site located within said polypeptide; and wherein post-translational type modification of said recognition motif modulates the rate of cleavage of said polypeptide by a protease with specificity for said protease site, b) contacting said sample and said engineered optical probe with a protease with specificity for said protease site, and c) detecting a change in at least one optical property of said engineered optical probe.