Patent ID: 9101692
Filing Date: 2015-08-11
Classification: A61L,A61P

Abstract:
1. A method of preparing an individually engineered implant ex vivo, the implant being a treated scaffold and based on either a natural or a synthetic scaffold which serves as a supporting matrix for tissue generation, wherein the implant is used for treatment of a patient with a tissue defect and a tissue injury, the method comprising the steps: (i) providing a natural or synthetic scaffold as a supporting matrix for growth of tissue cells; (ii) forming a stem cell preparation with autologous stem cells obtained from an anesthetized patient being treated, wherein the autologous stem cells are obtained from either the bone marrow or peripheral blood; iii) forming a healthy cell preparation comprising healthy cells biopsied from the defective and the injured tissue of the anesthetized patient, wherein the healthy cells function either as copy cells or co-differentiating cells supporting local epithelialization and stem cell differentiation in the defective tissue and the injured tissue; (iv) incubating the stem cell preparation of step (ii) with a booster composition comprising a boosting factor which stimulates stem cells and accelerates remodeling of tissue cells, wherein the boosting factor is selected from the group consisting of erythropoietin (EPO), thrombopoietin and human growth hormone (hGH); (v) loading and injecting the healthy cell preparation of step (iii) either onto or into the scaffold supporting matrix to form a pretreated scaffold matrix; vi) incubating the pretreated scaffold matrix of step (v) with the incubated stem cell preparation of step (iv) together with a combined composition comprising (a) a native recruitment factor for recruiting and increasing the availability of stem cells, wherein the native recruitment factor is selected from the group consisting of G-CSF and GM-CSF; (b) a native commitment factor that promotes differentiation of stem cells or its progenitor cells, wherein the native commitment factor is selected from the group consisting of TGFβ, VEGF, vitamin C, and vitamin E; and (c) the boosting factor which stimulates stem cells and accelerates remodeling of tissue cells of step (iv) to form the treated scaffold; (vii) carrying out steps (iv)-(vi) under sterile conditions in either a bioreactor chamber or a laminar air flow cabinet over a period of less than one hour.