Patent ID: 8993305
Filing Date: 2015-03-31
Classification: C12N,C12P,C12Y,Y02P

Abstract:
1. Recombinant microbial cells capable of producing isoprene from a carbon source, wherein the cells comprise one or more heterologous nucleic acids encoding a polypeptide having phosphoketolase activity and (i) one or more nucleic acids encoding one or more polypeptides of the complete MVA pathway and (ii) a heterologous nucleic acid encoding a polypeptide having isoprene synthase activity, wherein culturing of said recombinant cells in a suitable media provides for the production of isoprene, wherein the production of isoprene occurs without an increase in intracellular acetyl-CoA, and wherein the production of isoprene is increased relative to cells that do not comprise the one or more heterologous nucleic acids encoding a polypeptide having phosphoketolase activity.