Patent ID: 7247455
Filing Date: 2007-07-24
Classification: C07K,C12N,C12Q,G01N

Abstract:
1. A process for using a leader peptide associated with Streptococcus pyogenes DNase B enzyme to express a recombinant protein in a prokaryote comprising: (1) fusing a DNA coding for a protein to DNA coding for a leader peptide, the leader peptide having the sequence M-N-L-L-G-S-R-R-V-F-S-K-K-C-R-L-V-K-F-S-M-V-A-L-V-S-A-T-M-A-V-T-T-V-T-L-E-N-T-A-L-A-R (SEQ ID NO: 1), so that the fused DNA codes for the recombinant protein with a single reading frame with the leader peptide being at the amino-terminus of the protein; (2) inserting the fused DNA into an expression vector; (3) introducing the expression vector into the prokaryote; and (4) expressing the fused DNA in the prokaryote so that the recombinant protein is produced in a recoverable quantity.