Patent ID: 8178338
Filing Date: 2012-05-15
Classification: C12N

Abstract:
1. An expression vector comprising, in order from 5′ to 3′: a) a nucleotide sequence that encodes a transcriptional activator protein, wherein the transcriptional activator protein comprises an amino acid sequence having at least about 95% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO:3; b) a promoter that is inducible by the transcriptional activator protein, wherein the promoter comprises a nucleotide sequence having at least about 95% nucleotide sequence identity to the nucleotide sequence set forth in SEQ ID NO:1 or SEQ ID NO:2; and c) a multiple cloning site 3′ of and in operable linkage with the transcriptional activator protein-inducible promoter.