Patent ID: 6355860
Filing Date: 2002-03-12
Classification: C12N

Abstract:
A method for increasing the copy number of a target nucleic acid comprising the step of contacting a plant host cell under conditions suitable for transforming or transfecting said cell with an isolated polynucleotide comprising a plant-active Amplification Promoting Sequence (APS), said polynucleotide selected from the group consisting of:a) a polynucleotide comprising the sequence set forth in SEQ ID NO:1 or a fragment thereof; and b) a polynucleotide which hybridizes to a polynucleotide having the complement of the sequence set forth in step a), said hybridization occurring in 3Ã—SSC, 20 mM NaPO4 (pH 6.8) at 65Â° C. with washing in 0.2Ã—SSC at 65Â° C.; wherein said target nucleic acid is located within the genome of said plant host cell or is operably linked to the APS, and wherein said host cell has a greater copy number of the target nucleic acid compared to the copy number in cells in which the APS has not been introduced.