Patent ID: 6767566
Filing Date: 2004-07-27
Classification: C07K

Abstract:
A method for extraction of a proteinase inhibitor from raw potato tubers comprising the steps of:(a) preparing an alcohol-free extraction solution by adding to water, an organic acid in an amount to provide a concentration between about 0.5 weight percent and about 2.5 weight percent, and adding a salt to the water in an amount to provide between about 0.3 and 2.0 normality with regard to the salt; (b) adding the raw potato tubers to the extraction solution in a weight ratio of between about 1:1 and about 1:10 extraction solution of raw potato tubers; (c) comminuting the raw potato tubers in the extraction solution to reduce the mean particle size of the plant material to between about 100 microns and about 1500 microns thereby creating a slurry containing a liquid and a solid fraction; and (d) recovering said liquid fraction from said slurry to obtain a proteinase inhibitor.