Patent ID: 7604996
Filing Date: 2009-10-20
Classification: C12N,C12Q,G11C,H03K,Y10S

Abstract:
1. A method for multiplex detection of target nucleic acids comprising: a) providing a first substrate comprising greater than 50 different oligonucleotides linked to said first substrate through cleavable linkers, said greater than 50 different oligonucleotides having sequences different from each other, wherein said substrate comprises an array of discrete sites to which said greater than 50 different oligonucleotides are covalently linked; b) cleaving said linkers, thereby releasing said greater than 50 different oligonucleotides from said substrate thereby generating a pool of oligonucleotides comprising said greater than 50 different oligonucleotides; c) contacting said pool of oligonucleotides with a composition comprising different target nucleic acids, whereby said different target nucleic acids hybridize with said greater than 50 different oligonucleotides in said pool of oligonucleotides; d) modifying said greater than 50 different oligonucleotides in said pool of oligonucleotides hybridized with said different target nucleic acids to produce modified oligonucleotides, and e) contacting said modified oligonucleotides with a second substrate comprising probe oligonucleotides, said probe oligonucleotides having sequences different from each other and having sequences different from said greater than 50 different oligonucleotides released from said first substrate, whereby said target nucleic acids are detected.