Patent ID: 6232065
Filing Date: 2001-05-15
Classification: C12Q

Abstract:
A method, comprising:a) providing: i) a sample containing nucleic acid, ii) a first primer having a sequence of which at least a portion is at least partially complementary to a first conserved region of a gene from a multigene family, iii) a second primer having a sequence of which at least a portion is at least partially complementary to a second conserved region from said gene of said multigene family, said first and second conserved regions separated in each gene by a distance, said distance varying between a minimum distance and a maximum distance, said maximum distance differing by said minimum distance by less than 500 bases and iv) a polymerase and PCR reagents;b) preparing said nucleic acid from said sample under conditions so as to produce amplifiable nucleic acid;c) amplifying said nucleic acid with said first and second primers, said polymerase and said PCR reagents under conditions such that multiple amplified products are generated;d) isolating a portion of said amplified products to create a fragment mixture, said fragment mixture containing three or more amplified products in a size range, said range defined by a lower end and a higher end, said lower end defined by approximately said minimum distance and said higher end defined by approximately said maximum distance; ande) treating said fragment mixture with a plurality of restriction enzymes.