Patent ID: 7094399
Filing Date: 2006-08-22
Classification: A61K,A61P,C12N

Abstract:
1. A recombinant conditionally replicative adenovirus comprising a replication defective adenovirus having a deletion in its genome of an early gene which prevents replication of the adenovirus, said defective adenovirus comprising a transgene wherein said transgene encodes a pre-trans-splicing molecule (PTM) comprising: a) one or more target binding domains that target binding of the pre-trans-splicing molecule (PTM) to a target pre-mRNA expressed within a cancer cell; b) a 3′ splice region comprising a branch point a pyrimidine tract, and a 3′ splice acceptor site; c) a spacer region that separates the 3′ splice region from the target binding domain; and d) a nucleotide sequence to be trans-spliced to the target pre-mRNA wherein said nucleotide sequence encodes an adenovirus polypeptide required for adenovirus replication, which upon transplicing of the PTM to the target pr-mRNA results in a replicative adenovirus.