Patent ID: 6362393
Filing Date: 2002-03-26
Classification: A01H

Abstract:
A method of producing wheat plants from wheat microspores comprising:(a) selecting wheat spikes or flowers comprising microspores at a developmental stage amenable to androgenic induction; (b) subjecting said microspores to temperature stress to obtain stressed microspores; (c) contacting said microspores with an amount of a sporophytic development inducer effective to induce sporophytic development, said contacting step occurring before, during, after, or overlapping with any portion of said temperature stress step; (d) isolating said stressed and contacted microspores; (e) producing embryoids from the isolated, stressed, and contacted microspores of step (d) by coculturing said isolated, stressed and contacted microspores with either ovary-conditioned medium or at least one live plant ovary; and (f) regenerating wheat plants from the embryoids.