Patent ID: 6365351
Filing Date: 2002-04-02
Classification: A61K,A61P,C12N,C12Q

Abstract:
A method of detecting in a subject, the occurrence of a base-specific intracellular binding event involving a target mRNA produced by expression of a selected gene, comprising(a) administering to the subject a morpholino antisense oligomer compound having (i) from 8 to 40 nucleotides, including a targeting base sequence that is complementary to a region of the target mRNA, and (ii) uncharged, phosphorous-containing intersubunit linkages, in an amount effective to hybridize by Watson-Crick base pairing to a region of the target RNA with a Tm substantially greater than 37Â° C., (b) at a selected time after said administering, taking a sample of a body fluid from the subject, and (c) detecting in said sample the presence of a nuclease-resistant heteroduplex composed of the antisense oligomer and said target RNA region.