Patent ID: 9051595
Filing Date: 2015-06-09
Classification: C12Q,G01N

Abstract:
1. An in vitro method of screening for a bioactive compound exerting an inhibitory effect on a proteolytic activity of Malt1, the method comprising the steps: 1) exposing and/or contacting a compound to be screened to an oligomerized polypeptide having at least 50% sequence identity with SEQ. ID. NO. 48 and/or comprising the caspase-like domain of Malt1, the caspase-like domain being amino acids 336-572 of SEQ. ID. NO. 48, and the oligomerized polypeptide has Malt1-specific proteolytic activity, said oligomerized polypeptide being selected from a sample of cells comprising said oligomerized polypeptide, a sample of destroyed cells comprising said oligomerized polypeptide, and from recombinant, purified oligomerized polypeptide; 2) adding a marker peptide substrate, wherein said marker peptide substrate is susceptible of proteolytic cleavage by Malt1; 3) assessing Malt1-specific proteolytic activity by assessing the consumption of said marker peptide substrate when cleaved by said oligomerized polypeptide and/or assessing the presence of cleaved marker peptide substrate; and 4) selecting said compound of step 1 if said compound inhibits said proteolytic activity.