Patent ID: 8855941
Filing Date: 2014-10-07
Classification: C12Q,G16B

Abstract:
1. A method for examining prognosis of breast cancer comprising the steps of: (A) extracting RNA from a specimen collected from a subject, (B) preparing a determination sample using the RNA extracted in the step (A), (C) determining the expression level of each gene in the gene groups described in Table 1-1-1 and Table 1-2-1 using the determination sample obtained in the step (B), (D) analyzing the expression level of each gene determined in the step (C) comprising calculating solution D of a discriminant using the expression level and the discriminant is represented by the following formula (1): (E) examining prognosis of breast cancer, based on the analysis result obtained in the step (D), and predicting a poor prognosis when the solution D of the discriminant is a positive value, and a good prognosis when the solution D is 0 or a negative value, wherein i in the formula (1) shows a gene number provided to the nucleic acid described in Table 1-1 and Table 1-2, w and Σ i in the formula (1) shows the summation of each nucleic acid, and wherein j in the formula (2) shows the specimen number provided to each specimen, y ij in the formula (2) shows the standardized expression level in a specimen with specimen number j of a gene corresponding to the nucleic acid with gene number i, min in the formula (2) shows the minimum value of the value in parentheses, round in the formula (2) shows the value obtained by rounding the value in parentheses to the nearest whole number, abs in the formula (2) shows the absolute value of the value in parentheses, and y i in the formula (2) shows a standardized expression level of a gene corresponding to the nucleic acid with gene number i, the standardized expression level being obtained by standardization using the following formula (3): wherein x i in the formula (3) shows the expression level of a gene corresponding to the nucleic acid with gene number i, and u i in the formula (3) shows the average value of specimens of the expression level of a gene corresponding to the nucleic acid with gene number I, wherein Table 1-1-1 and Table 1-2-1 are: and wherein Table 1-1 and Table 1-2 are: