Patent ID: 7858367
Filing Date: 2010-12-28
Classification: A61K,C12N,C12Y

Abstract:
1. A recombinant hybrid virus, comprising: (a) a deleted adenovirus vector genome comprising the adenovirus 5′ and 3′ cis-elements for viral replication and encapsidation; a functional adenovirus genomic region selected from the group consisting of an adenovirus E1a region, E2a region, E4orf6 region, VA RNA region, and any combination of the foregoing; and further comprising a deletion in an adenovirus genomic region selected from the group consisting of: (b) a recombinant adeno-associated virus (AAV) vector genome flanked by the adenovirus vector genome sequences of (a), said recombinant AAV vector genome comprising (i) AAV 5′ and 3′ inverted terminal repeats, (ii) an AAV packaging sequence, and (iii) a heterologous nucleic acid sequence, wherein said heterologous nucleic acid sequence is flanked by the 5′ and 3′ AAV inverted terminal repeats of (i), and further wherein the AAV vector genome does not encode the AVV Rep or AAV capsid proteins, wherein upon infection of a 293 helper cell line, the recombinant hybrid virus is packaged into an AVV particle essentially without producing contaminating adenovirus.