Patent ID: 8338183
Filing Date: 2012-12-25
Classification: G01N

Abstract:
1. A method of measuring glycated proteins in a solution in which glycated/non-glycated proteins coexist comprising: adding labeling compounds capable of selectively binding to the glycated proteins, to a solution in which glycated/non-glycated proteins coexist; wherein the labeling compound is a boronic acid derivative including a substituent having at least one compound selected from the group consisting of viologen, dimethylferrocene (DMF), ferricinium, ferrocene monocarboxylic acid (FCOOH), 7,7,8,8-tetracyanoquino-dimethane (TCNQ), pyrroloquinoline quinine (PQQ), tetrathiafulvalene (TTF), N-methyl acidinium (NMA+), tetrathiatetracene (TTT), N-methylphenazinium (NMP+), hydroquinone, 3-dimethylaminobenzoic acid (MBTHDMAB), 3-methy1-2-benzothio-zolinone hydrazone, 2-methoxy-4-allylphenol, 4-aminoantipyrin (AAP), dimethylaniline, 4-aminoantipyrene, 4-methoxynaphthol, 3,3′,5,5′tetramethyl benzidine (TMB), 2,2-azino-di-[3-ethyl-benzthiazoline sulfonate], o-dianisidine, o-toluidine, 2,4-dichlorophenol, 4-amino phenazone and benzidine, filtering the solution to which labeling compounds are added, to remove glycated proteins bound to labeling compounds and non-glycated proteins from the solution through a filtering means; and quantifying by electrochemical determination, the residual labeling compounds, not bound to the glycated proteins in the filtered solution passing through the filtering means; wherein the amount of glycated proteins is determined from a difference between an added concentration of the labeling compounds in adding step and a quantified concentration of the labeling compounds in quantifying step.