Patent ID: 7138562
Filing Date: 2006-11-21
Classification: A01K,C07K,C12N

Abstract:
1. A method for introducing a polynucleotide contained within the genome of a recombinant retrovirus into the genome of a non-human mammalian zygote, comprising: a) providing a plurality of non-human mammalian zygotes, each having a plasma membrane and a zona pellucida, said plasma membrane and said zona pellucida defining a perivitelline space; b) providing an aqueous solution, which has been rendered cell-free by ultracentrifugation, wherein said cell-free aqueous solution comprises a polynucleotide contained within the genome of a recombinant retrovirus; c) introducing said solution comprising said polynucleotide contained within the genome into said perivitelline space by injection through a needle, under conditions which permit the integration of said polynucleotide contained within the genome of a recombinant retrovirus into the genome of said zygotes, such that said polynucleotide is stably maintained, thereby integrating the polynucleotide into the genome of at least twenty percent of said zygotes; and d) culturing said zygotes which have integrated the polynucleotide in their genomes.