Patent ID: 6087136
Filing Date: 2000-07-11
Classification: C12P

Abstract:
A microbial process for the preparation of D(-)-N-carbamoylphenylglycine from DL-5-phenylhydantoin which comprises culturing the strain Pseudomonas sp. having Accession No. and deposited with the American Type Culture Collection (ATCC), Manassas, Va., having a hydantoinase activity, in a medium for 16-20 hrs, the medium containing a supplement selected from the group consisting of 1-45% of molasses and 2% cornsteep liquor, separating the cells by centrifugation, treating the solid cells obtained with DL-5-phenylhydantoin in buffer at a pH range of 8-9.5, at a temperature in the range of 25-30.degree. C. for a period of 2-6 hrs, acidifying the mixture to obtain solid D(-)-N-carbamoylphenylglycine and separating the product by filtration.