Patent ID: 6709815
Filing Date: 2004-03-23
Classification: C07H,C12Q,Y02A

Abstract:
A method, comprising:a) providing: i) target nucleic acid comprising first and second non-contiguous single-stranded regions separated by an intervening region comprising a double-stranded region, wherein said target nucleic comprises at least a portion of Hepatitis C virus nucleic acid; ii) a bridging oligonucleotide capable of binding to said first and second non-contiguous single-stranded regions; iii) a second oligonucleotide capable of binding to a portion of said first non-contiguous single-stranded region; and iii) a cleavage agent; b) mixing said target nucleic acid, said bridging oligonucleotide, said second oligonucleotide, and said cleavage agent under conditions such that a cleavage structure is formed from said target nucleic acid, said bridging oligonucleotide, and said second oligonucleotide, and wherein either said second oligonucleotide or said bridging oligonucleotide is cleaved by said cleavage agent.