Patent ID: 9220803
Filing Date: 2015-12-29
Classification: A61K,A61L,A61P,C12N

Abstract:
1. A method for producing a mixture of native components, included in the group consisting of growth factors, extracellular matrix proteins and other substances produced by cell samples during normal conditions, consisting of the steps of: adding one or several different cell samples of human or animal origin to a bioreactor containing a nutrient medium, wherein a culturing medium is obtained; cell culturing during normal conditions, wherein the cell culturing is carried out under rotating condition; drawing off at least once the culturing medium including native components produced during the cell culturing; separating the native components from the culturing medium thereby obtaining a mixture of native components; and optionally, sterilizing, enriching, adding a carrier, and/or freeze-drying the separated native components; wherein the drawing off takes place continuously and the drawn-off culturing medium is replaced with new nutrient medium, said bioreactor having two or more interconnected chambers separated by a Millipore filter which allows for the culturing medium containing native components produced to diffuse into a second chamber or third chamber and so on and for separation of the cells from the culturing medium containing native components in the bioreactor, and wherein each cell sample originates from a tissue biopsy taken from cartilage and/or bone.