Patent ID: 7741051
Filing Date: 2010-06-22
Classification: A61K,C07K

Abstract:
1. An assay method for determining whether an agent binds to a MET ectodomain fragment in monomeric form or for determining whether said agent disrupts binding of said ectodomain fragment to hepatocyte growth factor/scatter factor (HGF/SF) or for determining whether said agent disrupts the binding of said ectodomain fragment to a fragment of HGF/SF which otherwise binds said ectodomain which comprises: (a) providing a MET ectodomain fragment in monomeric form, wherein said MET is SEQ ID NO: 1 or the A320V variant thereof and wherein said MET ectodomain fragment comprises amino acids 25-928 of the MET ectodomain or is an N-terminal fragment of amino acids 25-928 of the MET ectodomain having 495 amino acids; (b) providing an agent; and (c) determining whether the agent binds to said fragment or disrupts the binding of said fragment to hepatocyte growth factor/scatter factor (HGF/SF) or a fragment of HGF/SF which binds to said ectodomain, wherein said HGF/SF is human HGF/SF recorded as SEQ ID NO: 2.