Patent ID: 8883450
Filing Date: 2014-11-11
Classification: C07K,C12N

Abstract:
1. A method for producing a recombinant polypeptide of interest in an expression system, comprising introducing a signal peptide into the expression system, said signal peptide comprising at least 12 amino acids and having the sequence of formula (I): wherein X1 is a peptide containing from 3 to 6 amino acids, and i is 0 or 1, X2 is a peptide containing from 3 to 9 hydrophobic amino acids, X3 is a peptide containing from 3 to 5 amino acids, said peptide comprising at least 3 contiguous or non-contiguous leucines, X4 is a peptide containing from 2 to 5 amino acids selected from the group consisting of Ala, Thr, Ser, Gln, Ile, and Met, X5 is Ala or Val, X6 is Asn or His, and X7 is Ala or Cys, provided that: when said signal peptide originates from a natural precursor of a specific protein, said polypeptide of interest is different from said protein, and irrespective of the polypeptide of interest which can be bound to one of said signal peptides in order to be secreted in the extracellular medium.