Patent ID: 6773918
Filing Date: 2004-08-10
Classification: C12Q

Abstract:
A method for enhancing transcriptional activation of a reporter gene under the control of a promoter regulated by a DmpR protein, said DmpR protein comprising a sensor domain, in bacteria selected from the group consisting of Pseudomonas and Escherichia coli, in response to phenols and substituted phenols, selected from the group consisting of phenol, 2-chlorophenol, 2,4-dichlorophenol, 4-chloro-3-methylphenol, 2,4-dimethylphenol, 4-nitrophenol, and 2 nitrophenol, over the transcriptional activation exhibited by wild type bacteria of the same strain, said method comprising the steps of subjecting a DNA encoding the DmpR protein sensor domain to mutagenic polymerase chain reaction, ligating a mutated sensor domain fragment generated thereby into a DNA encoding the DmpR protein from which a corresponding sensor domain fragment has been removed, and testing the bacteria for enhanced response to said phenols and substituted phenols over the response thereto for wild type bacteria without altering other domains.