Patent ID: 8399213
Filing Date: 2013-03-19
Classification: C07K,C12Q,G01N

Abstract:
1. A method of detecting intracellular protein tyrosine phosphatase (PTP) activity comprising: (a) obtaining one or more peptide(s), wherein said peptide(s) comprises a cell-permeable tag formed by N-terminal fusing a coumaryl-amino acid residue to a cell-permeable peptide, and wherein said coumaryl-amino acid residue may be phosphorylated coumaryl-amino propionic acid (pCAP), unphosphorylated coumary-amino propionic acid (CAP), or a fluorinated derivative thereof; (b) internalizing the peptides into cells and; (c) detecting PTP intracellular activity by monitoring cell fluorescence of the cells of step (b).