Patent ID: 7081336
Filing Date: 2006-07-25
Classification: C12Q

Abstract:
1. A composition for detection of a subject nucleic acid comprising, a. a first nucleic acid probe that hybridizes to a first nucleic acid target sequence on the subject nucleic acid, forms a stem-loop structure when not bound to the first nucleic acid target sequence, and incorporates a resonance energy transfer donor moiety; and b. a second nucleic acid probe that hybridizes to a second nucleic acid target sequence on the subject nucleic acid, forms a stem-loop structure when not bound to the second nucleic acid target sequence, and incorporates a resonance energy transfer acceptor moiety, wherein the first nucleic acid target sequence and the second nucleic acid target sequence are separated by a number of nucleotides on the subject nucleic acid such that a resonance energy transfer signal from interaction between the donor moiety of the first nucleic acid probe and the acceptor moiety of the second nucleic acid probe can be detected to determine hybridization of both the first nucleic acid probe and the second nucleic acid probe to the subject nucleic acid.