Patent ID: 8642734
Filing Date: 2014-02-04
Classification: C07K

Abstract:
1. A method of producing polymers of an isolated spider silk protein, comprising the steps of: (i) providing a spider silk protein consisting of from 170 to 760 amino acid residues and comprising: (ii) providing a solution of said spider silk protein in a liquid medium at pH 6.4 or higher and/or an ion composition that prevents polymerisation of said spider silk protein, optionally involving removal of lipopolysaccharides and other pyrogens; (iii) adjusting the properties of said liquid medium to a pH of 6.3 or lower and an ion composition that allows polymerisation of said spider silk protein; (iv) allowing the spider silk protein to form polymers in the liquid medium, said liquid medium having a pH of 6.3 or lower and an ion composition that allows polymerisation of said spider silk protein; and (v) isolating the spider silk protein polymers from said liquid medium.