Patent ID: 6703544
Filing Date: 2004-03-09
Classification: C07K,C12N

Abstract:
A method for reducing the quantity of a soybean seed storage protein in soybean seeds comprising:(a) constructing a chimeric gene comprising: (i) a nucleic acid fragment encoding a promoter that is functional in the cells of soybean seeds; (ii) a nucleic acid fragment encoding all or a portion of a soybean seed storage protein placed in sense or antisense orientation relative to the promoter of (i); and (iii) a transcriptional termination region; (b) creating a transgenic soybean cell by introducing into a soybean cell the chimeric gene of (a); and (c) growing the transgenic soybean cells of step (b) under conditions that result in expression of the chimeric gene of step (a) wherein the quantity of one or more members of a class of soybean seed storage protein subunits is reduced when compared to soybeans not containing the chimeric gene of step (a).