Patent ID: 6586247
Filing Date: 2003-07-01
Classification: C12N

Abstract:
A medium for cultivating an animal embryo or fertilized ovum, which consists essentially of the following aqueous solutions (A) and (B) which are mixed before use:(A) 50 to 100 parts by volume of a neutral aqueous solution having a pH of 7.0 to 7.5 and containing the following components: sodium lactate1.0 to 2.0mlsodium chloride5.0 to 7.0gpotassium chloride0.3 to 0.5gcalcium chloride.2H2O0.1 to 0.4gmagnesium sulfate.7H2O0.03 to 0.1gPhenol Red1.0 to 3.0mgsodium bicarbonate1.5 to 3.0gHEPES2.0 to 5.0gglucose0.1 to 0.8g;and(B) 1 part by volume of an acidic aqueous solution having a pH of 4.0 to 5.5 and containing the following components: pyruvic acid1.0 to 3.0mlglutathione0.3 to 0.8gL-cysteine hydrochloride4.0 to 7.0gascorbic acid3.0 to 7.0gNAD0.03 to 0.07gcepharothin0.3 to 0.7gfosfomycin0.5 to 1.5g.