Patent ID: 9206447
Filing Date: 2015-12-08
Classification: C12N,C12P,C12Y,Y02E

Abstract:
1. A recombinant yeast host cell comprising at least two engineered modifications comprising: (i) a heterologous dihydroxy-acid dehydratase Fe—S cluster protein expressed in the cytosol of the recombinant yeast host cell, wherein the heterologous dihydroxy-acid dehydratase Fe—S cluster protein is a polypeptide having an amino acid sequence that is at least 90% identical to SEQ ID NO: 114 using the Clustal W method of alimment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0,1, and Gonnet 250 series of protein weight matrix over the full length of the protein sequence and has a specific activity of at least 0.2 μmol min (ii) an endogenous Fe—S cluster protein with reduced expression, wherein the endogenous Fe—S cluster protein is dihydroxy-acid dehydratase.