Patent ID: 9206404
Filing Date: 2015-12-08
Classification: A01K,C07K,C12N

Abstract:
1. A method for making a deletion in an endogenous IgM gene, the method comprising: introducing mRNA into an isolated rat cell, wherein the mRNA comprises (i) mRNAs encoding a first zinc finger nuclease (ZFN) that binds to a first target site in an endogenous rat IgM gene wherein the first ZFN comprises a cleavage domain and a zinc finger protein, wherein the first zinc finger protein-comprises the following recognition helices in the following order: (ii) mRNA encoding a second ZFN that binds to a second target site in the endogenous rat IgM gene, wherein the second ZFN comprises a cleavage domain and a zinc finger protein comprising the following recognition helices in the following order: such that the first and second ZFNs are expressed, dimerize and cleave the endogenous rat IgM gene and non-homologous end joining (NHEJ) occurs causing the cell to have a deletion in the endogenous rat IgM gene.