Patent ID: 6165723
Filing Date: 2000-12-26
Classification: C12Q

Abstract:
A method for detecting a target nucleic acid fragment in a specimen obtained from a patient by in situ hybridization comprising the steps:a) depositing a sample of the specimen onto a slide;b) fixing the sample onto the slide with fixative, the fixative comprising either methanol-acetic acid at a ratio of from 99:1 to 80:20, or formalin-acetic acid at a ratio of from 99:1 to 80:20;c) contacting nucleic acids of the fixed sample with a probe complex that specifically hybridizes to the target nucleic acid fragment, under hybridization conditions;d) rinsing non-hybridized probe complex from the sample;e) staining the rinsed sample with Evans Blue; andf) visually detecting hybridized probe complex by microscopy, with the presence of the hybridized probe complex being an indication of the presence of the target nucleic acid fragment.