Patent ID: 7728106
Filing Date: 2010-06-01
Classification: A61K,A61P,C07K,C12N,C12P

Abstract:
1. A method of simultaneously adding two homogeneous oligosaccharide N-glycans adjacent to a V3 domain of HIV gp120, the method comprising: providing a precursor peptide comprising the V3 domain of HIV gp120 and two Asn amino acid residues wherein the two Asn residues are positioned adjacent to two cysteine residues of the V3 domain and wherein each of the two Asn amino acid residues comprise a N-acetylglucosamine (GlcNAc) moiety and act as a GlcNAc-peptide acceptor; and conducting a transglycosylation reaction between an excess of a pre-assembled homogeneous oligosaccharide oxazoline and the two GlcNAc-peptide acceptors under the catalysis of Endo-A or Endo-M to generate two homogeneous oligosaccharide N-glycans positioned at the Asn amino acid residues adjacent to the V3 domain.