Patent ID: 6531124
Filing Date: 2003-03-11
Classification: A61K,C07K,C12N

Abstract:
A method of providing a glucagon-like peptide 1 related peptide to a mammal, said method comprising the steps of:a) obtaining primary cells from the mammal; b) transfecting primary cells obtained in (a) with a DNA construct comprising exogenous DNA encoding a glucagon-like peptide 1 related peptide and additional DNA sequences sufficient for expression of the exogenous DNA in the primary cells, thereby producing transfected primary cells which express the exogenous DNA encoding a glucagon-like peptide 1 related peptide; c) culturing a transfected primary cell which expresses the exogenous DNA encoding a glucagon-like peptide 1 related peptide produced in (b), under conditions appropriate for propagating the transfected primary cell which expresses the exogenous DNA encoding a glucagon-like peptide 1 related pepitide, thereby producing a clonal cell strain of transfected secondary cells from the transfected primary cell; d) culturing the clonal cell strain of transfected secondary cells produced in (c) under conditions appropriate for and sufficient time for the clonal cell strain of transfected secondary cells to undergo a sufficient number of doublings to provide a sufficient number of transfected secondary cells to produce glucagon-like peptide 1 related peptide; and e) introducing transfected secondary cells produced in (d) into the mammal in sufficient number to produce glucagon-like peptide 1 related peptide in the mammal.