Patent ID: 6849410
Filing Date: 2005-02-01
Classification: C12Q

Abstract:
1. A method for detecting a DNA polymorphism in a double strand DNA, said method comprising the steps of (a) to (e) below: (a) contacting (i) a double strand DNA comprising a test polymorphic site, (ii) an oligonucleotide probe that hybridizes to a region comprising said polymorphic site in said double strand DNA, and (iii) a homologous recombination protein under reaction conditions where a triple strand DNA complex is formed, (b) removing the homologous recombination protein from the triple strand DNA complex formed in the step (a), thereby generating a triple strand DNA, (c) conducting heat treatment of the triple strand DNA generated by removing the homologous recombination protein, under conditions where the oligonucleotide probe is released from said triple strand DNA, when the test polymorphic site in the double strand DNA is not complementary to a corresponding site in said oligonucleotide probe, (d) determining the existence or the absence of an oligonucleotide probe that binds to the double strand DNA to form the triple strand DNA, (e) judging that (i) SNP exists in the DNA region complementary to the oligonucleotide probe in the target double strand DNA when no oligonucleotide probe is detected, (ii) no SNP exists in the DNA region complementary to the oligonucleotide probe in the target double strand DNA when the existence of oligonucleotide probe that binds to triple strand DNA is detected.