Patent ID: 6649365
Filing Date: 2003-11-18
Classification: C12Q

Abstract:
A method for determining the presence or absence of a peptidase activity, comprising:adding, as a tracer agent, at least one compound of formula (I): Xâ€”NHâ€”Râ€ƒâ€ƒ(I) in which X represents an optionally substituted indol-3-yl group and R represents the acyl residue of an amino acid or of a peptide, the amine group(s) present in R being optionally in protected form, to a culture medium, anddetecting formation of a coloured or fluorescent product in said culture medium; wherein said tracer agent reveals the presence of either a peptidase activity in a microorganism culture, or the presence of a microorganism or of a group of microorganisms expressing such an activity in said medium, by formation of a coloration or a fluorescence in said medium, and wherein said tracer agent is not a compound of formula (I) for which X represents a 5-bromoindol-3-yl group and R represents a leucyl or alanyl residue.