Patent ID: 9017974
Filing Date: 2015-04-28
Classification: A61K,A61P,A61Q,B01J,C07F,C09B,C12P

Abstract:
1. A method for producing a composition of porphyrin-phospholipids conjugate from a mixture of sn-1 and sn-2 regioisomers of porphyrin-phospholipid conjugates, each of said regioisomers comprising one porphyrin, porphyrin derivative or porphyrin analog covalently attached to a lipid side chain at one of the sn-1 or the sn-2 positions of one phospholipid, wherein the composition has a defined regioisomeric ratio of sn-1:sn-2 porphyrin-phospholipid conjugate, the method comprising incubating the mixture of regioisomers with an enzyme that selectively cleaves one of the sn-1 or the sn-2 regioisomers until the defined regioisomeric ratio is achieved; wherein the porphyrin, porphyrin derivative or porphyrin analog in the porphyrin-phospholipid conjugate is selected from the group consisting of hematoporphyrin, protoporphyrin, tetraphenylporphyrin, a pyropheophorbide, a bacteriochlorophyll, chlorophyll a, a benzoporphyrin, a tetrahydroxyphenyl chlorin, a purpurin, a benzochlorin, a naphthochlorin, a verdin, a rhodin, a keto chlorin, an azachlorin, a bacteriochlorin, a tolyporphyrin, a benzobacteriochlorin, an expanded porphyrin and a porphyrin isomer; and wherein the enzyme is selected from Lipase from