Patent ID: 8951806
Filing Date: 2015-02-10
Classification: B01L,G01N

Abstract:
1. A method for determining an analyte in a liquid sample, comprising: applying the sample to a test element comprising: a reagent zone comprising (1) a labeled conjugate comprising an analyte binding partner and a label and (2) a tagged conjugate comprising an analyte binding partner and a tagging substance, wherein the labeled conjugate and the tagged conjugate are present in the reagent zone in migratable form and are dissolved by the liquid sample; a detection zone comprising a first permanently immobilized binding partner for the tagging substance; and a control zone comprising a second permanently immobilized binding partner for the tagging substance and a permanently immobilized binding partner for the labeled conjugate, wherein the permanently immobilized binding partner for the labeled conjugate competes with the analyte for binding to the labeled conjugate, determining the presence or amount of the analyte in the detection zone by the detecting the presence or amount of signal associated with the label in the detection zone, and determining whether the analyte has a very high concentration that causes a Hook effect by detecting the absence of a signal in the detection zone and the control zone.