Patent ID: 6239262
Filing Date: 2001-05-29
Classification: B01D,C07K,C12N

Abstract:
A method for purifying a protein by displacement chromatography comprising:(a) configuring a chromatographic system selected from the group consisting of hydrophobic interaction chromatography and reversed phase liquid chromatography, said chromatographic system having a hydrophobic stationary phase, for operation in displacement mode;(b) selecting for use as a displacer a surface-active compound having molecular weight of less than 10,000 daltons and greater than 0 daltons and having a greater affinity for the hydrophobic stationary Phase than the protein;(c) loading the protein on the hydrophobic stationary phase;(d) displacing the protein from the stationary phase with said surface-active compound and moving the protein through the chromatographic system ahead of the displacer; and(e) collecting the displaced Protein.