Patent ID: 8753821
Filing Date: 2014-06-17
Classification: C12Q,Y10T

Abstract:
1. A mixture or kit comprising: a) five nucleic acid primers each having a length of 23-30 nucleotides, wherein each of the five primers comprise one of SEQ ID Nos.: 1-5, each of which primer hybridizes to a sequence within the nucleic acid of one or more Mollicutes; and b) four PNA probes, each of which probe comprises a probing nucleobase sequence of 12-20 nucleobase containing subunits, wherein the probing nucleobase sequence is selected from the group consisting of SEQ. ID. NO: 6, SEQ. ID. NO: 7, SEQ. ID. NO: 8, SEQ. ID. NO: 9, the complement to SEQ. ID. NO: 6, the complement to SEQ. ID. NO: 7, the complement to SEQ. ID. NO: 8 and the complement to SEQ. ID. NO: 9, and wherein each probe hybridizes to a target sequence within the nucleic acid of one or more Mollicutes.