Patent ID: 6531276
Filing Date: 2003-03-11
Classification: A61K,A61P,C07K,C12N,C12Q,Y10S

Abstract:
A method for detecting the presence of a polynucleotide comprising a human immunodeficiency virus (HIV) genomic sequence in a nucleic acid sample obtained from a physiological sample, which method comprises the steps of:(a) combining said nucleic acid sample with a single-stranded nucleic acid probe comprising a sequence of at least about 20 contiguous bases selected from the nucleotide sequence shown in FIG. 4 and complementary to said HIV genomic sequence comprised in said polynucleotide, said probe not forming a duplex with HTLV-I and -II nucleic acid sequences under conditions of stringency for hybridization under which said probe forms a duplex with said polynucleotide; and (b) determining duplex formation between said probe and nucleic acid present in said sample.