Patent ID: 8431366
Filing Date: 2013-04-30
Classification: C07H,C12Q

Abstract:
1. A method of DNA amplicon fragment analysis comprising: i) annealing an oligonucleotide primer to a denatured DNA template such that, the oligonucleotide primer anneals to a complementary oligonucleotide sequence on a strand of the denatured DNA template to form a primer-template complex, wherein the oligonucleotide primer comprises at least one modified purine nucleobase comprising the structure: ii) extending the primer portion of the primer-template complex in the presence of extendable nucleotide triphosphates and non-extendable nucleotide triphosphates to form one or more DNA amplicon fragments; and iii) detecting the DNA amplicon fragments, wherein the oligonucleotide primer comprising at least one modified purine nucleobase no more than 3 nucleotides from the 3′ terminus of the oligonucleotide primer significantly reduces the formation of primer-dimer amplicons.