Patent ID: 7556922
Filing Date: 2009-07-07
Classification: C12Q

Abstract:
1. A method of determining the sequence of a polynucleotide, the method comprising: (i) contacting a polynucleotide template with a nucleotide-selective, processive enzyme in a reaction mix that is rate-altering for the processive movement of the enzyme for a specified nucleotide; (ii) recording the processive movement of the enzyme along the polynucleotide template to provide an enzyme movement trace that differs at the site of the specified nucleotide versus non-specified nucleotides; repeating steps (i) and (ii) with reaction conditions that are rate-altering for each nucleotide; combining and aligning said enzyme movement traces, wherein the sequence of said polynucleotide template is derived from base specific alteration of movement shown in said enzyme movement traces.