Patent ID: 9109047
Filing Date: 2015-08-18
Classification: C07K,D01F

Abstract:
1. A method for generating high molecular ordered fibrilar structures by directing ordered assembly of fibrous proteins, the method comprising: a. providing at least one fibrous protein monomer selected from the group consisting of spider-silk, resilin, elastin, silk-worm silk, collagen and mussel byssus protein; b. providing at least one heterologous cellulose binding domain (CBD); c. attaching said at least one fibrous protein monomer provided in step (a) to said CBD provided in step (b), thereby obtaining a polypeptide comprising an amino acid sequence of a fibrous protein monomer attached to a heterologous CBD; d. providing a solution of the polypeptide obtained in step (c) under conditions which allow folding of said fibrous protein to generate said fibrilar structures; e. sonicating the solution of the polypeptide obtained in step (d) under conditions which allow folding of said fibrous protein to generate said fibrilar structures; and f. optionally recovering or isolating said fibrilar structures from said solution or liquid medium, thereby obtaining isolated high molecular ordered fibrilar structures.