Patent ID: 9222076
Filing Date: 2015-12-29
Classification: A61P,C12N

Abstract:
1. A process for generating a skin layer patch or a dressing for a patient in need thereof, characterized in that it comprises the steps of: obtaining, from a patient requiring a skin layer patch or dressing, an autologous skin sample and a blood sample; digestion of the autologous skin sample for 18 hours; obtaining autologous serum from the patient's blood sample, separating the digested autologous skin into two layers, a superficial epidermis layer and a deep layer of dermis; culturing and replication of keratinocytes in the autologous serum for at least 72 h, wherein the keratinocytes are obtained from the superficial layer of the digested autologous skin; culturing and replication of fibroblasts obtained from the deep layer of the digested autologous skin; seeding the cultured fibroblasts on a collagen mesh; and seeding the cultured keratinocytes on the fibroblast-seeded collagen mesh and then culturing the keratinocyte-seeded collagen mesh for 24 h to obtain the skin layer patch or dressing for the patient.