Patent ID: 7351816
Filing Date: 2008-04-01
Classification: A61K,C07K

Abstract:
1. An isolated nucleic acid molecule encoding a humanized antibody that specifically binds IgE comprising: (a) a VH domain and a VL domain, wherein: (b) a VH domain and a VL domain, wherein: (i) the VH domain comprises the VH domain of SEQ ID NO:8 in which the alanine residue at position 61 of SEQ ID NO:8 is replaced by an asparagine residue, the aspartic acid residue at position 62 of SEQ ID NO:8 is replaced by a proline residue, and the phenylalanine residue at position 68 of SEQ ID NO:8 is replaced by an isoleucine residue, in which said positions correspond to Kabat numbering 60, 61 and 67, respectively, and (ii) the VL domain comprises the VL domain of SEQ ID NO:9; or (c) a VH domain and a VL domain, wherein: (i) the VH domain comprises the VH domain of SEQ ID NO:8 in which the valine residue at position 49 of SEQ ID NO:8 is replaced by a methionine residue, the alanine residue at position 50 of SEQ ID NO:8 is replaced by a isoleucine residue, the alanine residue at position 61 of SEQ ID NO:8 is replaced by an asparagine residue, the valine residue at position 64 of SEQ ID NO:8 is replaced by an leucine residue, the phenylalanine residue at position 68 of SEQ ID NO:8 is replaced by an isoleucine residue, the isoleucine residue at position 70 of SEQ ID NO:8 is replaced by a valine residue, the methionine residue at position 83 of SEQ ID NO:8 is replaced by a leucine residue, and the leucine residue at position 86 of SEQ ID NO:8 is replaced by an alanine residue, in which said positions correspond to Kabat numbering 48, 49, 60, 63, 67, 69, 82 and 82c, respectively, and (ii) the VL domain comprises the VL domain of SEQ ID NO:9 in which the alanine residue at position 13 of SEQ ID NO:9 is replaced by a valine residue, the valine residue at position 19 of SEQ ID NO:9 is replaced by an alanine residue, the valine residue at position 62 of SEQ ID NO:9 is replaced by an isoleucine residue, the leucine residue at position 82 of SEQ ID NO:9 is replaced by a valine residue, and the valine residue at position 108 of SEQ ID NO:9 is replaced by a leucine residue, in which said positions correspond to Kabat numbering 13, 19, 58, 78 and 104, respectively.