Patent ID: 6239328
Filing Date: 2001-05-29
Classification: C07K,C12N

Abstract:
A method of making recombinant plants with reduced silencing of expression of foreign genes therein, comprising:(a) providing a monocot plant cell capable of regeneration;(b) transforming, the plant cell with a DNA construct comprising an expression cassette, which construct comprises, in the 5' to 3' direction, a transcription initiation region, a structural gene positioned downstream from the transcription initiation region and operatively associated therewith, a first matrix attachment region positioned 5' to the structural gene, and a second matrix attachment region positioned 3' to the structural gene, to produce a transformed plant cell; then(c) propagating a plant from said transformed plant cell to provide a first transgenic plant; and then(d) sexually propagating said first transgenic plant to provide a subsequent transgenic plant having reduced silencing of expression of foreign genes therein.