Patent ID: 6787323
Filing Date: 2004-09-07
Classification: C12Q,G01N

Abstract:
A method for determining vascular endothelial growth factor (VEGF) activity in a sample, said method comprising the steps of:a) contacting a sample to be assayed for VEGF activity with a stable HeLa cell line wherein the stable HeLa cell line comprises: 1) a reporter vector having; an expressible reporter element and a DNA binding site disposed adjacent thereto and, 2) a chimeric transactivator vector comprising a gene encoding a phosphorylatable protein that can be phosphorylated by MAPK and a DNA binding domain which specifically binds to the DNA binding site, and 3) an expression vector encoding a gene for a VEGF receptor; and b) detecting expression of the reporter element, wherein expression of the reporter element indicates VEGF activity wherein the sample comprises cells, tissue, tissue extracts or combinations thereof.