Patent ID: 6984308
Filing Date: 2006-01-10
Classification: G01N

Abstract:
1. A method for simultaneously analyzing an aqueous mixture of quinones and hydroquinones in a biological fluid comprising the steps of: a) passing said mixture through a liquid chromatographic column for achieving time-spaced separation of the quinones and hydroquinones eluted from the column; b) oxidizing said hydroquinones by passing them through a coulometric guard cell having a voltage of at least about +700 mV; and c) passing said quinones and oxidized hydroquinones through an analytical cell consisting essentially of a series of at least two coulometric electrodes, wherein the first electrode operates in a reductive mode at about −650 mV and the second electrode mixture; said at least two coulometric electrodes being arranged in series and defining collectively at least one flow channel for said mixture.