Patent ID: 6475794
Filing Date: 2002-11-05
Classification: C12N,C12Q

Abstract:
A method of detecting mutations in a gene of interest of a nonrodent mammal, comprising the steps of:fusing cells of a non-rodent mammal to rodent cell recipients of a chromosomally stable cell line to form non-rodent mammal-rodent cell hybrids; selecting for fused cell hybrids by selecting for a first marker contained on a rodent chromosome and for a second marker contained on a first non-rodent mammalian chromosome, to form a population of fused cell hybrids; detecting among the population of fused cell hybrids a subset of hybrids which are haploid for a second non-rodent mammalian chromosome which is not the same chromosome as the first non-rodent mammalian chromosome and which was not selected; analyzing said subset of hybrids to detect a mutation in an mRNA or protein product of a gene or in said gene, wherein the gene resides on the second non-rodent mammalian chromosome.