Patent ID: 7803543
Filing Date: 2010-09-28
Classification: C12Q

Abstract:
1. A method for determining whether a target polynucleotide sequence contained in a nucleic acid sample has nucleotide variation(s) in a selected region thereof, comprising providing a pair of a first primer and a second primer which allows the formation of a PCR product comprising a sequence covering that of the selected region of the target polynucleotide sequence via a PCR process, providing a labeled peptide nucleic acid probe comprising a detectable moiety and comprising a sequence that fully complements to the sequence of the selected region of a reference target polynucleotide sequence comprising no nucleotide variation(s) therein, such that hybridization of the labeled peptide nucleic acid probe to the selected region of said reference target polynucleotide sequence results in the formation of a first duplex having a first melting temperature; admixing the labeled peptide nucleic acid probe and said pair of the first and second primers with a nucleic acid sample to form a mixture;