Patent ID: 6428971
Filing Date: 2002-08-06
Classification: C12N,C12Q,C12Y,G01N

Abstract:
A process for determining teichoic acid polymerase activity in a sample, the method comprising:combining CDP-glycerol, water, a lipoteichoic acid substrate from a bacterium selected from the group consisting of Staphylococcus aureus, Enterococcus faecalis, and Bacillus subtilis, and the sample to form a mixture; incubating the mixture under conditions effective for a teichoic acid polymerase in the sample, to cause the incorporation of at least one glycerol-3-phosphate moiety into the lipoteichoic acid substrate to form a lipoteichoic acid product having at least one additional glycerol-3-phosphate moiety incorporated therein, wherein the teichoic acid polymerase is encoded by DNA having at least about 70% homology with SEQ ID NO:1; and determining the presence or absence of the lipoteichoic acid product.