Patent ID: 6479246
Filing Date: 2002-11-12
Classification: G01N

Abstract:
A method of performing a competitive binding immunoassay for determining in vitro the presence of a specified blood antigen phenotype in a sample of whole blood, where said blood antigen phenotype comprises platelet blood antigens, and wherein said method comprises:(a) contacting a sample of whole blood with a predetermined amount of antibody specific to said blood antigen phenotype, so as to bind the antibody to any of said antigen present in the sample of whole blood; (b) contacting the mixture of step (a) with a substrate upon which antigen of said phenotype has been immobilized such as to bind any remaining unbound antibody to said immobilized antigen, thereby forming an immobilized antigen-antibody complex; (c) washing the immobilized antigen-antibody complex; and (d) detecting the amount of immobilized antigen-antibody complex, wherein the amount of immobilized antigen-antibody complex indicates the presence of said blood antigen phenotype.