Patent ID: 8361755
Filing Date: 2013-01-29
Classification: C12N,C12P,Y02E

Abstract:
1. A method for performing starch liquefaction in a slurry that includes granular starch and phytate, the method comprising: (a) preparing a slurry comprising granular starch and thin stillage, (b) contacting the slurry with a thermostable phytase and a thermostable alpha-amylase, wherein the thermostable phytase comprises at least one amino acid substitution selected from N37Y, H160R and A374P according to the sequence set forth in SEQ ID NO:1, (c) performing primary liquefaction and secondary liquefaction, and (d) using dextrins produced in step (c) for fermentation, wherein the pH of the slurry is not adjusted in any of steps (a)-(d).