Patent ID: 7595176
Filing Date: 2009-09-29
Classification: C07H,C12N,C12Q

Abstract:
1. A method of quantifying the amount of a plurality of different Dehalococcoides species present in a sample comprising, contacting said sample with: (i) a probe comprising the sequence 5′TCCTCAGTTCGGATTGCAGGCTGAA3′ (SEQ ID NO: 1); (ii) a first primer comprising the sequence 5′CTGGAGCTAATCCCCAAAGCT3′ (SEQ ID NO: 2); and (iii) a second primer comprising the sequence 5′CAACTTCATGCAGGCGGG3′ (SEQ ID NO: 3); and performing real time PCR on said sample to quantify the amount of said plurality of different