Patent ID: 6451260
Filing Date: 2002-09-17
Classification: B01D,B01J,B01L,C12Q,G01N

Abstract:
A method for producing a filter device comprising a support and a microporous element as a filter and/or absorption material, wherein the microporous element is generated within an aperture of the device by a process comprising the steps of:(a) applying a substance present in a liquid phase wherein said liquid phase is a solution or suspension of one or more crosslinking monomers present in a solvent, said monomers further comprising a diamine and a polyepoxide, said substance further comprising solid porous or non-porous microparticles from 0.01 &mgr;m to 500 &mgr;m in size, wherein said microparticles contain an effector molecule capable of sequestering an ion, selectively binding to a solid support, binding to a preselected antigenic determinant or being a ligand, said effector molecule: (i) comprises an enzyme or a remotely detectable moiety, or (ii) is capable of sequestering an ion and is selected from the group consisting of calmodulin, metallothionein, a fragment thereof, or an amino acid sequence rich in at least one of glutamic acid, aspartic acid, lysine, and arginine, or (iii) is capable of binding to a preselected antigenic determinant and is an antibody or fragment thereof, or (iv) is capable of selective binding to a solid support and is selected from the group consisting of GST, His-tag, or Lex-A, or (v) is a ligand and is Ni-NTA; and (b) causing solidification in spongy form of at least part of the substance wherein said solidification is caused by polymerizing said monomers.