Patent ID: 6946128
Filing Date: 2005-09-20
Classification: C07K,C12N

Abstract:
1. A protease conjugate comprising a microbial protease and one or more addition moieties wherein the microbial protease comprises a first epitope region, a second epitope region, and a third epitope region, wherein each addition moiety is covalently attached to an epitope protection position of the microbial protease, wherein: (a) the epitope protection positions for the first epitope region are selected from the group of positions corresponding to positions consisting of 17 and 89 of the amino acid sequence of subtilisin BPN′ set forth in SEQ ID NO:1; (b) the epitope protection positions for the second epitope region are selected from the group of positions corresponding to positions consisting of 52 and 134 of the amino acid sequence of subtilisin BPN′ set forth in SEQ ID NO:1; and, (c) the epitope protection positions for the third epitope region are selected from the group of positions corresponding to positions consisting of 155 and 265 of the amino acid sequence of subtilisin BPN′ set forth in SEQ ID NO:1.