Patent ID: 8071288
Filing Date: 2011-12-06
Classification: C12N,G01N

Abstract:
1. A method for detecting the presence of a target molecule suspected of being contained in a test mixture, wherein said target molecule is a protein, the method comprising; a) providing a solid support, said solid support comprising a photoreactive nucleic acid ligand having specific affinity for said target protein, said photoreactive nucleic acid ligand binding specifically to said target molecule through non-Watson-Crick interactions; b) contacting said solid support with said test mixture suspected of containing said target molecule, wherein a nucleic acid ligand-target molecule complex is formed if said target molecule is present; c) irradiating said solid support, wherein said nucleic acid ligand-target molecule complex photocrosslinks; d) removing non-specifically bound material from said solid support; e) contacting said solid support with one or more reagents that label proteins with a detectable moiety; and f) detecting the presence of said target molecule by detecting the presence of said detectable moiety on said solid support.