Patent ID: 8773126
Filing Date: 2014-07-08
Classification: G01R

Abstract:
1. A nuclear magnetic resonance signal measuring method, comprising the steps of: providing a sample including (A) a measurement target having an isotope-labeled compound with a first nucleus and at least two nuclei that are different from the first nucleus and that (i) can transmit nuclear magnetic resonance signals, (ii) have mutually different nuclear magnetic resonance frequencies and (iii) are bonded to each other, said compound being isotope-labeled at a higher ratio than a natural isotope abundance ratio, and (B) a non-measurement target having a nuclide of the same type as the first nucleus of the isotope-labeled compound, the nuclide being contained in a compound which is not isotope-labeled; irradiating to the sample a plurality of pulses generated by a nuclear magnetic resonance apparatus, the nuclear magnetic resonance apparatus having (i) a unit for observing the nuclear magnetic resonance signal of the first nucleus contained in the isotope-labeled compound, which has passed magnetization transfer from the first nucleus to a second nucleus, magnetization transfer from the second nucleus to a third nucleus, magnetization transfer from the third nucleus to the second nucleus, and magnetization transfer from the second nucleus to the first nucleus, among three nuclei comprising said two nuclei, and (ii) a unit for adding positional information to the nuclear magnetic resonance signal of the first nucleus contained in the isotope-labeled compound; and determining with the nuclear magnetic resonance apparatus the nuclear magnetic resonance signal of the first nucleus contained in the isotope-labeled compound the nuclear magnetic resonance signal being generated by irradiating the plurality of pulses, wherein a nuclear magnetic resonance signal of the nuclide contained in said non-measurement target is decreased relative to the nuclear magnetic resonance signal of the first nucleus.