Patent ID: 8852864
Filing Date: 2014-10-07
Classification: C12Q

Abstract:
1. A method for analyzing a nucleic acid, which comprises: (a) contacting a target nucleic acid with a plurality of nucleic acid probes under conditions in which probes having a nucleotide sequence substantially complementary to a subsequence of the target nucleic acid (complementary nucleotide sequence) can hybridize to the target nucleic acid, wherein: (b) ligating the ends of the complementary nucleotide sequences of probe pairs that are adjacent to one another when hybridized to the target nucleic acid; (c) joining the ends of detector regions in proximity to one another, thereby forming a linked probe molecule; (d) passing the linked probe molecule through the pore of a nanopore device; and (e) determining the base sequence of the linked probe molecule, whereby the target nucleic acid is analyzed.