Patent ID: 6558954
Filing Date: 2003-05-06
Classification: A61K,C12N

Abstract:
A method for converting a drug-resistant eukaryotic cell to a drug-sensitive eukaryotic cell, the method comprising bringing into contact(a) the drug-resistant eukaryotic cell, wherein the drug-resistant eukaryotic cell contains a targeted RNA molecule involved in conferring drug resistance to the eukaryotic cell, and (b) a RNAase P ribozyme that binds an eukaryotic external guide sequence, or an eukaryotic external guide sequence, wherein the RNAase ribozyme or external guide sequence cleaves or promotes RNAase P mediated cleavage of the RNA molecule, respectively, at a specific cleavage site thereby converting the drug-resistant eukaryotic cell to a drug-sensitive eukaryotic cell.