Patent ID: 8753874
Filing Date: 2014-06-17
Classification: C12M,G01N

Abstract:
1. A DNA chip, comprising: a flat carrier; an array of spots containing catcher molecules, each spot being assigned a microelectrode arrangement for detecting binding events between the catcher molecules and target molecules applied via an analyte solution, wherein the microelectrode arrangement is at least a two-pole system, the microelectrode arrangement being at least partially embedded in a hydrophilic reaction layer which is permeable to target molecules and in which immobilized catcher molecules are distributed three-dimensionally, wherein the catcher molecules are configured to cause the binding events, the hydrophilic reaction layer having a thickness approximately in the range of 1L to 5 L, L being the sum of electrode width and electrode spacing, the electrode width and the electrode spacing being approximately 1 μm, wherein the chip is configured for application of an alternating voltage in a megahertz frequency, and wherein the chip is configured to detect a capacitance change caused by the binding events within the hydrophilic reaction layer with a megahertz frequency applied to the electrodes.