Patent ID: 6323007
Filing Date: 2001-11-27
Classification: A61K,A61Q,C11D,C12N,C12P,C12Y

Abstract:
An isolated polypeptide having 2,6-.beta.-D-fructan hydrolase activity, selected from one of:(a) a polypeptide having an amino acid sequence of amino acids 32 to 923 of SEQ ID NO:1, amino acids 25 to 1277 of SEQ ID NO:3, or amino acids 29 to 943 of SEQ ID NO:5;(b) an allelic variant of a polypeptide having an amino acid sequence which has at least 90% identity to amino acids 32 to 923 of SEQ ID NO:1, amino acids 25 to 1277 of SEQ ID NO:3, or amino acids 29 to 943 of SEQ ID NO:5;(c) a polypeptide encoded by a nucleic acid sequence which hybridizes under high stringency conditions with (i) nucleotides 94 to 2769 of SEQ ID NO:2 or 73 to 3824 of SEQ ID NO:4 or 85 to 2832 of SEQ ID NO:6, (ii) the cDNA sequence comprising nucleotides 94 to 2769 of SEQ ID NO:2 or 73 to 3824 of SEQ ID NO:4 or 85 to 2832 of SEQ ID NO:6, or (iii) a complimentary strand of (i) or (ii) wherein the hybridization conditions composes hybridization at 42.degree. C. in a solution comprising 5.times.SSPE, 200 .mu.g/ml sheared and denatured salmon sperm DNA, and 0.3% SDS followed by washing at 65.degree. C. in a solution comprising 2.times.SSC and 0.2% SDS; and(d) a fragment of (a) or (b) that has 2,6-.beta.-D-fructan hydrolases activity.