Patent ID: 6475746
Filing Date: 2002-11-05
Classification: A01K,A61K,A61P,C07K,C12N,Y10S

Abstract:
A method of obtaining a composition which comprises determining whether a chemical compound binds to (a) a human 5-HT1D receptor expressed on the surface of a mammalian cell transfected with a vector adapted for expressing the receptor in the cell or (b) a human 5-HT1D receptor present in a membrane preparation obtained from such a mammalian cell, and if the chemical compound binds to the receptor in (a) or (b), recovering the chemical compound free of any 5-HT1D receptor and admixing the chemical compound with a carrier; wherein the 5-HT1D receptor is (1) a 5-HT1D-1 receptor which has an amino acid sequence encoded by a DNA sequence which is 65% or more homologous to the receptor-encoding DNA sequence contained in plasmid pcEXV-8-30-84 (ATCC Accession No. 40790) and exhibits essentially the same binding properties as those shown in Table 1 for the human 5-HT1D-1 receptor; or (2) a 5-HT1D-2 receptor which has an amino acid sequence encoded by a DNA sequence which is 65% or more homologous to the receptor-encoding DNA sequence contained in plasmid pSVL-11 (ATCC Accession No. 40791) and exhibits essentially the same binding properties as those shown in Table 1 for the human 5-HT1D-2 receptor.