Patent ID: 6110698
Filing Date: 2000-08-29
Classification: C12Q

Abstract:
A method for identifying compounds that inhibit the transcription promotion function of Drosophila ultraspiracle protein (Usp), said method comprising:(i) providing a transformed yeast cell comprising:(a) a Usp binding partner wherein the Usp binding partner is a member of a nuclear receptor family;(b) Usp or derivatives thereof containing an apparent domain structure typical of the nuclear steroid receptor family, including an A/B (transactivation) domain, a C (DNA binding/dimerization/transactivation) domain, a D (nuclear localization) domain, and an E (dimerization) domain capable of forming a functional Usp--Usp binding partner complex with said Usp binding partner; and(c) a reporter gene, wherein expression of said reporter gene is transcriptionally activated by said functional Usp--Usp binding partner complex and whose expression in yeast is readily detectable in said transformed yeast cell;(ii) incubating said transformed yeast cell in the presence of a test compound to form a test culture, and in the absence of a test compound to form a control culture; and(iii) monitoring expression of said reporter gene in said test and control cultures.