Patent ID: 6022864
Filing Date: 2000-02-08
Classification: A61K,A61P,C07K,C12N

Abstract:
A method of immunizing a host against disease caused by infection with respiratory syncytial virus (RSV), which comprises administering to said host an effective amount of a plasmid vector comprising:a first nucleotide sequence encoding an RSV F protein that generates antibodies, said first nucleotide sequence being selected from the group consisting of:a nucleotide sequence having SEQ ID NO:1,a nucleotide sequence having SEQ ID NO:3,a nucleotide sequence encoding an amino acid sequence having SEQ ID NO:2, anda nucleotide sequence encoding an amino acid sequence having SEQ ID NO:4,a promoter sequence operatively coupled to said first nucleotide sequence for expression of said RSV F protein in said host, anda second nucleotide sequence located between said first nucleotide sequence and said promoter sequence and comprising a pair of splice sites to prevent aberrant mRNA splicing and to increase expression of said RSV F protein when expressed in vivo from said vector in said host.