Patent ID: 6686164
Filing Date: 2004-02-03
Classification: A61K,C12N,C12Y,C40B

Abstract:
A method of producing a protein variant, comprising the steps of:(a) subjecting a random peptide display package library to one or more antbodies to identify peptides that bind to the one or more of the antibodies; (b) identifying one or more epitope patterns by aligning the peptides that bind to the one or more of the antibodies with each other; (c) obtaining a three-dimensional structure of a parent protein; (d) identifying the one or more epitope patterns on the three-dimensional structure of the parent protein; (e) identifying an epitope area of amino acids situated within 5 Ã¥ of any amino acid of the one or more epitope patterns of the parent protein; and (f) modifying one or more amino acids identified in step (e) of the parent protein to form the protein variant, wherein the protein variant retains functionality of the parent protein and has a lower immunogenicity than the parent protein.