Patent ID: 8202691
Filing Date: 2012-06-19
Classification: C12Q

Abstract:
1. A method for preparing and analysing a population of fragmented polynucleotide sequences having a substantially uniform size comprising: (a) binding at least one protection molecule to at least one polynucleotide sequence; (b) cleaving the at least one polynucleotide sequence to generate a plurality of polynucleotide fragment sequences of substantially uniform size; (c) amplifying the polynucleotide fragments, wherein the amplification is performed on a solid support, wherein the amplification produces an array of locations, each location comprising multiple copies amplified from a single template; and (d) sequencing the polynucleotide fragments, wherein a strand of each of the templates is sequenced, the strand of each of the templates is converted into a double stranded template using strand resynthesis, and wherein the resynthesized template is used for a second round of sequencing, thereby analysing a population of fragmented polynucleotide sequences having a substantially uniform size.