Patent ID: 7439023
Filing Date: 2008-10-21
Classification: B01D,B01J,C12N

Abstract:
1. A method of isolating a nucleic acid from a sample comprising the nucleic acid, comprising: contacting the sample with a bifunctional material containing an amino group and a carboxyl group at a first pH to bind the nucleic acid to the bifunctional material, the bifunctional material being positively charged at the first PH; and releasing the nucleic acid at a second pH which is higher than the first pH, wherein the bifunctional material has formula 1 or 2, wherein n is an integer from 1 to 10, A is wherein x is O, CO, S, SO 2 , CH 2 , C(CH 3 ) 2 or C(CF 3 ) 2 , (wherein a carbonyl group and a carboxyl group may be substituted on any carbon position except for a linking portion of the rings), and Y is a nitrogen-containing heterocyclic base, wherein R R 1 is an integer from 1 to 30,000.