Patent ID: 6057121
Filing Date: 2000-05-02
Classification: B82Y,C07K,C12N,C12P,C12Q,G01N

Abstract:
A method for catalyzing a stereochemical reaction wherein a reactant comprising a stereoisomer contained within a mixture of stereoisomers is converted to at least one product, said method comprisingcontacting said mixture with at least one stereoselective monoclonal antibody capable of catalytically increasing the rate of conversion of said stereoisomer to said product, said contacting being performed under conditions wherein a complex is formed between said monoclonal antibody and said stereoisomer, wherein said stereoisomer is catalytically converted to said product and said product is released from said complex, said monoclonal antibody having been produced by a process comprising the steps of:(a) generating a plurality of monoclonal antibodies to an antigen selected from the group consisting of:(i) the reactant;(ii) the reactant bound to a peptide or other carrier molecule;(iii) a reaction intermediate;(iv) an analog of the reactant;(v) an analog of the product, wherein the monoclonal antibody so generated is capable of binding to the reactant or a reaction intermediate; and(vi) an analog of a reaction intermediate;wherein said antigen comprises a single stereoisomer or a mixture of stereoismers; and(b) screening said plurality of monoclonal antibodies to identify a stereoselective monoclonal antibody which catalyzes the reaction.