Patent ID: 6740521
Filing Date: 2004-05-25
Classification: C12N,G01N

Abstract:
A cellwhich is obtained by transfer of a promoter containing a heat shock factor binding DNA sequence and a transcriptional regulatory sequence which functions in response to stress induction of a protein as a transcriptional regulatory factor binding site, as well as a reporter gene under the control of said promoter into a chromosome, said reporter gene being connected, on the downstream side thereof, to the SV40pA signal without any intervening intron, and which is usable for detecting chemicals or natural toxins which disrupt or disturb homeostasis in organisms, by measuring a protein inducing activity of said reporter gene, wherein the promoter containing the heat shock factor binding DNA sequence and the transcriptional regulatory sequence which functions on the occasion of stress induction of a protein as the transcriptional regulatory factor binding site, is an HSP47 promoter containing the heat shock factor binding DNA sequence and an SP1 factor binding sequence.