Patent ID: 9057097
Filing Date: 2015-06-16
Classification: C12Q

Abstract:
1. A kit for a recombinase polymerase amplification process of DNA amplification of a target nucleic acid molecule comprising: (a) one or more freeze dried pellets each comprising the following reagents in the following concentrations, which-unless otherwise indicated can be the concentration either when reconstituted or when freeze dried; (1) 1.5%-5% (weight/lyophilization mixture volume) of polyethylene glycol; (2) 2.5%-7.5% weight/volume of trehalose; (3) 0-60 mM Tris buffer; (4) 1-10 mM DTT; (5) 150-400 μM dNTPs; (6) 1.5-3.5 mM ATP; (7) 100-350 ng/μL uvsX recombinase; (8) optionally 50-200 ng/μL uvsY; (9) 150-800 ng/μL gp32; (10) 30-150 ng/μL Bsu polymerase or Sau polymerase; (11) 20-75 mM phosphocreatine; and (12) 10-200 ng/μL creatine kinase.