Patent ID: 7670812
Filing Date: 2010-03-02
Classification: C12N,C12P

Abstract:
1. A method of producing glycogen comprising a step of: allowing a branching enzyme having the ability to synthesize glycogen to act on a substrate in a solution to produce a glycogen, wherein the substrate is an α-glucan being linked mainly with α-1,4-glycosides bond and having a degree of polymerization of 4 or more; wherein the substrate is debranched starch, debranched dextrin or enzymatically synthesized amylose; the number-average molecular weight of saccharides in the solution before initiation of the reaction is more than 180 but not more than 150,000; the weight-average molecular weight of the glycogen is 1,000,000 Da or more; the weight-average molecular weight of the product produced by subjecting the glycogen to reaction with pullulanase in an amount of 50 U/g substrate at 60° C. C. for 30 minutes is 500,000 Da or more as analyzed by the MALLS method; the weight-average molecular weight of the product produced by subjecting the glycogen to reaction with α-amylase in an amount of 300 U/g substrate at 37° C. for 30 minutes is 500,000 Da or more as analyzed by the MALLS method; in the method, neither α-glucan phosphorylase nor glycogen synthase is used; and wherein the branching enzyme comprises an amino acid sequence having at least about 95% identity with the amino acid sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16 and SEQ ID NO: 18.