Patent ID: 7972840
Filing Date: 2011-07-05
Classification: C12M,C12N

Abstract:
1. A method of in vitro culturing an organism tissue or cell, which comprising the following steps: placing a culture medium and a microporous body into a container such that only a part of the microporous body is immersed in the culture medium, wherein the microporous body has communicating pores having a pore diameter of 0.02-20 μm; placing the organism tissue or cell to be cultured on a surface of the microporous body where the culture medium has been transferred upwardly via communicating pores in the interior of the microporous body having the capillary attraction; allowing the organism tissue or cell to absorb the culture medium from the surface of the microporous body; growing the organism tissue or cell inside the container, wherein the culture medium, the microporous body, the container, and a lid or a plug for the container are sterile prior to placing the organism tissue or cell on the surface of the microporous body under aseptic conditions, the container is covered with the lid or the plug under aseptic conditions after placing the organism tissue or cell inside, the container is covered with the lid or the plug during culturing, and gases and/or medium optionally added to the container after placing the organism tissue or cell inside are sterile and introduced under aseptic conditions.