Patent ID: 6156530
Filing Date: 2000-12-05
Classification: G01N

Abstract:
A method of diagnosing thrombophilia in a mammal comprising: contacting, in vitro, a sample of blood or plasma of an individual suspected of having a thrombophilic disorder with fixed endothelial cells or outer membranes of fixeda) determining the rate of coagulum formation or the time required for the formation of a predetermined amount of coagulum, comparing the rate or time with a reference value from blood or blood plasma obtained from normal individuals, wherein if the rate is faster or the coagulation time is shorter, the sample is assessed to come from an individual having disposition for thrombosis orb) halting the coagulation, determining the amount of coagulum, and comparing the amount of coagulum with reference values of amounts of coagulum obtained from normal individuals, wherein if the amount of coagulum is greater than the reference values the sample is assessed to come from an individual having disposition for thrombosis, orc) halting the coagulation, allowing coagulum dissolving reactions to continue, determining the amount of dissolved coagulum, comparing the amount of dissolved coagulum with reference values of dissolved coagulum obtained from normal individuals, wherein if the dissolved amount of coagulum is smaller than the reference values the sample is assessed to come from an individual having disposition for thrombosis, ord) halting the coagulation, allowing coagulum dissolving reactions to continue, determining the amount of remaining coagulum, comparing the amount of remaining coagulum with reference values of remaining coagulum obtained from normal individuals, wherein if the amount of remaining coagulum is greater than the reference values the sample is assessed to come from an individual having disposition for thrombosis.