Patent ID: 8759096
Filing Date: 2014-06-24
Classification: B01L,C12M

Abstract:
1. A method for culturing tissue in vitro utilizing a microfluidic chip adapted to input and output a medium having a predetermined flow speed, comprising a substrate having a surface, and at least a tissue culture area formed on the surface of the substrate, having a single or a plurality of microfluidic channels formed by a plurality of connected geometrical structures having a predetermined depth, the microfluidic channel having an inlet and an outlet at two ends thereof for inputting the medium and outputting the same respectively, and at least an air-exchange hole being formed on the bottom of the microfluidic channel, wherein the method comprising the steps of: (a) providing a polymer membrane being cultured with a plurality of cells thereon; (b) providing a mechanical force to suck the polymer membrane through the air-exchange hole, so that the polymer membrane is attached to the single or the plurality of microfluidic channels of the microfluidic chip; (c) arranging a tissue on the polymer membrane; and (d) inputting a medium with a predetermined flow speed to the inlet of the microfluidic channel.