Patent ID: 7892796
Filing Date: 2011-02-22
Classification: C12Q

Abstract:
1. A method for assaying for one or more target oligonucleotides in a sample comprising: (a) amplifying the one or more target oligonucleotides with a reaction mixture that comprises at least one amplification primer complementary to the one or more target oligonucleotides and forming an amplification product, wherein the at least one amplification primer comprises at least one non-standard base; (b) contacting the amplification product of step (a) with at least one tagged primer that comprises a tagging sequence with at least one non-standard base and a sequence complementary to the amplification product; (c) extending the at least one tagged primer and forming an extension product, wherein the extending step is carried out in the presence of a non-standard nucleotide triphosphate that is complementary to the at least one non-standard base of the at least one amplification primer; (d) contacting the extension product with an oligonucleotide bound to a solid support, wherein the oligonucleotide comprises a molecular recognition sequence that is complementary to the tagging sequence; and (e) detecting specific hybridization between the extension product and the oligonucleotide bound to the solid support.