Patent ID: 6277643
Filing Date: 2001-08-21
Classification: G01N,Y10T

Abstract:
A method for the detection of protein on polyacrylamide gels comprising the steps of staining the polyacrylamide gels with a counter-dye composition containing an acidic organic dye and a basic organic dye, wherein the acidic organic dye and the basic organic dye are selected from the group consisting of Zincon and Ethy violet, Zincon and Methyl violet, Zincon and Meldola's blue, Coomassie brilliant blueR and Phenosafranin, Coomassie brilliant blue G and Methyl orange, Calconcarboxylic acid and Rhodamine B, and Eriochrome black T and Rhodamine B and protein complexes with the counter-dye composition, and observing protein-counter-dye complexes.