Patent ID: 7604956
Filing Date: 2009-10-20
Classification: G01N,Y10S

Abstract:
1. A super-sensitive sandwich immunoassay which can quantitate proteins with a detection level at less than 100 fg/ml by reducing biological background interference in the specimen to be tested comprising the steps of: (a) coating a testing substrate with an Abs (b) super stringent washing the testing substrate; (c) treating the testing substrate with a blocking reagent; (d) super stringent washing the testing substrate; (e) depositing a sample of physiologic fluid on the testing substrate; (f) super stringent washing the testing substrate; (g) applying an Abs (h) super stringent washing the testing substrate; (i) applying a streptavidin based reagent onto the testing substrate; (j) super stringent washing the testing substrate; and (k) measuring and generating a signal representative of ultra-low amounts of labeled antibodies at detection levels less than 100 fg/ml, wherein the level of protein is ascertained using one or more of the following: wherein super-sensitivity is obtained by use of super-stringent washing wherein the protein detection ability is diminished by a factor greater than 3; and wherein the supersensitive washing is obtained using one or more of the following procedures: