Patent ID: 9222944
Filing Date: 2015-12-29
Classification: G01N

Abstract:
1. A method of screening for a salty taste modulating substance, which comprises the step of contacting a test substance with a cell that expresses a Kv3.2 protein, and comparing observed cation influx into the cell with cation influx into the cell observed when the test substance is not contacted with the cell, wherein (a) when the cation influx is larger when the test substance is contacted with the cell that expresses a Kv3.2 protein as compared to when the test substance is not contacted with the cell that expresses a Kv3.2 protein, the test substance is correlated with enhancing salty taste, and (b) when the cation influx is smaller when the test substance is contacted with the cell that expresses a Kv3.2 protein as compared to when the test substance is not contacted with the cell that expresses a Kv3.2 protein, the test substance is correlated with inhibiting salty taste, wherein the Kv3.2 protein has a sequence identity of at least 95% to the sequence of SEQ ID NO: 6, and is able to constitute a cation channel of which activity changes according to change of extracellular sodium ion concentration.