Patent ID: 8137924
Filing Date: 2012-03-20
Classification: A61P,C07K,C12Y,G01N

Abstract:
1. An isolated polynucleotide molecule encoding a molecular construct, wherein the construct is capable of anchoring to a plasma membrane or a vesicular membrane of a cell, the construct comprising (1) a linker peptide, (2) a FRET pair comprising a donor fluorophore moiety and an acceptor fluorophore moiety, and (3) a membrane-anchoring domain that directs the anchoring of the construct to the plasma or vesicular membrane of the cell, wherein the linker peptide comprises a cleavage recognition site of a botulinum neurotoxin selected from the group consisting of synaptobrevin, syntaxin and SNAP-25, and wherein the membrane-anchoring domain comprises a fragment that contains the palmitoylation site (residues 83-120) of SEQ ID NO: 7, or the transmembrane domain (residues 95-116) of SEQ ID NO: 8.