Patent ID: 7592440
Filing Date: 2009-09-22
Classification: C07H,C12N,C12Q

Abstract:
1. A method of identifying at least one antisense sequence for inhibiting expression of a preselected target nucleic acid comprising: providing a set of two or more candidate antisense sequences, wherein each candidate antisense sequence consists of 12 to 25 nucleobases and is complementary to the preselected target nucleic acid sequence; eliminating from the set of candidate antisense sequences, any candidate antisense sequences comprising negative predictor sequence motif 5′-GGGG-3′; eliminating from the set of candidate antisense sequences, any candidate antisense sequences comprising negative predictor sequence motif 5′-GGA-3′; selecting from the set of candidate antisense sequences, at least one test sequence, comprising positive predictor sequence motif 5′-CCAC-3′; synthesizing and testing at least one test antisense oligonucleotide having a test sequence; and thereby identifying at least one antisense sequence for inhibiting expression of the preselected target nucleic acid.