Patent ID: 6737513
Filing Date: 2004-05-18
Classification: A01K,A61K,C07K

Abstract:
In a composition for generating an immune response in a mammal against a pathogen that comprises an antigen from a pathogen in a pharmaceutical carrier, the improvement comprising inclusion in said composition of a polypeptide selected from the group consisting of Macrophage Derived Chemokine (MDC) having the amino acid sequence of residues 1-69 of SEQ ID NO: 2, and MDC polypeptide analogs thereof that retain MDC chemoattractant activity for CCR4+ TH2 cells,wherein the MDC polypeptide analog comprises an amino acid sequence that is encoded by a polynucleotide that hybridizes to a DNA comprising the non-coding strand complementary to nucleotides 92 to 298 of SEQ ID NO: 1, under the following stringent hybridization conditions: hybridization at 42Â° C. in a hybridization solution comprising 5Ã—SSC, 20 mM NaPO4, pH 6.8, 50% formamide; and washing at 42Â° C. in a wash solution comprising 0.2Ã—SSC, and wherein the polypeptide is included in the composition in an amount effective to stimulate CCR4+ TH2 cell chemotaxis in the mammal.