Patent ID: 8546539
Filing Date: 2013-10-01
Classification: A61K,A61P,C07K

Abstract:
1. An altered antibody or antigen-binding fragment thereof that binds to soluble TLR4, the TLR4/MD2 complex, or both soluble TLR4 and the TLR4/MD2 complex, the antibody or antigen-binding fragment thereof comprising: (i) a variable heavy chain region comprising a heavy chain CDR1 region comprising the amino acid sequence GGYSWH (SEQ ID NO: 23), a heavy chain CDR2 region comprising the amino acid sequence YIHYSGYTDFNPSLKT (SEQ ID NO: 24), and a heavy chain CDR3 region comprising the amino acid sequence of KDPSDAFPY (SEQ ID NO: 80); (ii) a variable light chain region comprising a light chain CDR1 region comprising the amino acid sequence RASQSISDHLH (SEQ ID NO:28), a light chain CDR2 region comprising the amino acid sequence YASHAIS (SEQ ID NO:29), and a light chain CDR3 comprising the amino acid sequence of QQGHSFPLT (SEQ ID NO: 83); and (iii) an Fc region comprising two mutations compared to a starting antibody, and wherein one mutation is a substitution with serine at EU amino acid position 325, and one mutation is a substitution with phenylalanine at EU amino acid position 328.