Patent ID: 6074852
Filing Date: 2000-06-13
Classification: A61K,A61P,C07K,C12N,Y10S

Abstract:
A method for producing an hepatitis C virus (HCV) qlycoprotein having mannose-terminated glycosylation, wherein less than about 10% of the total N-linked carbohydrate on said HCV glycoprotein is sialic acid, and further wherein said HCV qlycoprotein is selected from the group consisting of a glycoprotein expressed from the E1 region of HCV and a glycoprotein expressed from the E2 region of HCV comprising the steps of:growing a mammalian host cell transformed with a structural gene encoding an HCV glycoprotein expressed from either the E1 region of HCV or the E2 region of HCV in a suitable culture medium;causing expression of said structural gene under conditions inhibiting sialylation; andisolating said HCV qlycoprotein from cell culture by contacting said HCV glycoprotein with a mannose-binding protein specific for mannose-terminated glycoproteins.