Patent ID: 8241692
Filing Date: 2012-08-14
Classification: A23J,A23L,A23V,C07K

Abstract:
1. A process of preparing a canola protein isolate, which comprises: (a) extracting an oil seed meal to cause solubilization of protein in the oil seed meal and to form an aqueous protein solution having a pH of about 5 to about 6.8; (b) separating the aqueous protein solution from the residual oil seed meal; (c) increasing the protein concentration of said aqueous protein solution while maintaining the ionic strength substantially constant by using a selective membrane technique to provide a concentrated protein solution; (d) diluting said concentrated protein solution into chilled water having a temperature of below about 15° C. to cause the formation of discrete protein particles in the aqueous solution in the form of micelles; (e) settling the protein micelles to form an amorphous, sticky, gelatinous, gluten-like protein micellar mass; (f) separating the protein micellar mass from supernatant, the protein micellar mass having a protein content of at least about 90 wt. % (N×6.25); (g) forming an aqueous solution of the protein micellar mass; (h) acidifying the aqueous solution of protein micellar mass to isoelectrically precipitate therefrom a protein isolate having a protein content of at least about 90 wt. %, (N×6.25); and (i) separating the precipitated protein isolate from supernatant.