Patent ID: 6197518
Filing Date: 2001-03-06
Classification: C12Q

Abstract:
A method of detecting Fusarium head blight resistance in a wheat cell, said method comprising:providing genomic DNA extracted from the wheat cell;providing a primer set selected from the group consisting of:primer set A: a forward primer selected from the group consisting of: Forward Fhb1 primer (SEQ ID No. 1), contiguous primers thereof, noncontiguous primers thereof and homologous primers thereof; and a reverse primer selected from the group consisting of: Reverse Fhb1 primer (SEQ ID No. 2), contiguous primers thereof, noncontiguous primers thereof and homologous primers thereof; orprimer set B: a forward primer selected from the group consisting of: Forward Fhb2 primer (SEQ ID No. 3), contiguous primers thereof, noncontiguous primers thereof and homologous primers thereof; and a reverse primer selected from the group consisting of: Reverse Fhb2 primer (SEQ ID No. 4), contiguous primers thereof, noncontiguous primers thereof and homologous primers thereof; orprimer set C: a forward primer selected from the group consisting of: Forward Fhb3 primer (SEQ ID No. 5), contiguous primers thereof, noncontiguous primers thereof and homologous primers thereof; and a reverse primer selected from the group consisting of: Reverse Fhb3 primer (SEQ ID No. 6), contiguous primers thereof, noncontiguous primers thereof and homologous primers thereof; orcombinations of primer set A, primer set B and primer set C.combining the genomic DNA and the primer set with reagents suitable for DNA amplification, thereby forming a reaction mixture;incubating the reaction mixture under conditions wherein the DNA is amplified, thereby producing a DNA fragment of a given molecular weight; anddetermining the molecular weight of the DNA fragment, wherein use of primer set A results in synthesis of a DNA molecule of a first molecular weight when Fhb1 is present on the genomic DNA or the synthesis of a DNA molecule of a second molecular weight when Fhb1 is absent on the genomic DNA; use of primer set B results in synthesis of a DNA molecule of a third molecular weight when Fhb2 is present on the genomic DNA or the synthesis of a DNA molecule of a fourth molecular weight when Fhb2 is absent on the genomic DNA; and use of primer set C results in synthesis of a DNA molecule of a fifth molecular weight when Fhb3 is present on the genomic DNA or the synthesis of a DNA molecule of a sixth molecular weight when Fhb3 is absent on the genomic DNA.