Patent ID: 7575900
Filing Date: 2009-08-18
Classification: C12N,C12P,C12Y

Abstract:
1. A process for producing a non-reducing saccharide having a trehalose structure as an end unit, trehalose, or a saccharide composition comprising the same, comprising: subjecting a reducing partial starch hydrolysate to the action of (1) a non-reducing saccharide-forming enzyme which forms said non-reducing saccharide from a reducing partial starch hydrolysate, and has an optimum temperature of over 40° C. but below 60° C. to produce a non-reducing saccharide, wherein said non-reducing saccharide-forming enzyme comprises the amino acid sequence of SEQ ID NO: 1, consist of the amino acid sequence of SEQ ID NO: 1, and optionally (2) a trehalose-releasing enzyme which hydrolyzes the non-reducing saccharide having a trehalose structure as an end unit to release said trehalose from the rest of said non-reducing saccharide, and has an optimum temperature of over 45° C. but below 60° C. to produce trehalose, wherein said trehalose-releasing enzyme comprises the amino acid sequence of SEQ ID NO: 9 or consists of the amino acid sequence of SEQ ID NO: 9; and collecting the produced non-reducing saccharide, trehalose, or a saccharide composition comprising the same from the resulting reaction mixture.