Patent ID: 6130366
Filing Date: 2000-10-10
Classification: C12N

Abstract:
A plant comprising in its nuclear genome a chimaeric DNA sequence, comprising:a.) a nucleic acid sequence coding for a chimaeric protein or polypeptide comprising:i.) a transit peptide of a cytoplasmic precursor of a chloroplast protein or chloroplast polypeptide of a plant species; andii.) a protein or polypeptide of interest which is different from the polypeptide or protein normally associated with said transit peptide in a cytoplasmic precursor, wherein said transit peptide is located in the amino-terminal part of said chimaeric protein or polypeptide; andb.) a promoter upstream of said nucleic acid sequence recognized by polymerases endogenous to said plant; wherein the chimaeric DNA sequence can be expressed in cells of said plant under control of said promoter, and said protein or polypeptide of interest is translocated into chloroplasts of cells of said plant.