Patent ID: 6251661
Filing Date: 2001-06-26
Classification: B01J,C12N

Abstract:
A method for producing a reaction capsule comprising: concurrently extruding a plurality of concentric layers from a plurality of nozzles into a cooling solution, wherein an inner nozzle extrudes an aqueous reaction mixture comprising components required for synthesizing a polynucleotide or a polypeplide; an intermediate nozzle extrudes a viscous fluid that is not miscible with water; and an outer nozzle extrudes an encapsulating polymer layer formed of polysaccharide, protein, or a mixture thereof to form a reaction capsule; andisolating the reaction capsule from the cooling solution, wherein the flow rate from the nozzles is controlled to produce a capsule having an approximate diameter of 0.1 to 10.0 mm.