Patent ID: 8017389
Filing Date: 2011-09-13
Classification: A61P,C12N

Abstract:
1. A method of preparing a purified isolated cell population enriched in cardiac stem cells or cardiac progenitor cells, comprising: (a) obtaining a cardiac tissue sample that comprises (1) cardiac stem cells or cardiac progenitor cells and (2) fibroblasts; then (b) culturing said cardiac tissue sample for a time sufficient to allow the fibroblasts to adhere to the culture dish; then (c) collecting cells from the culture dish that migrate above the adhered fibroblasts of step (c); then (d) separating Sca-1-expressing cardiac stem cells or Sca-1-expressing cardiac progenitor cells from the collected cells; then (e) culturing the separated Sca-1-expressing cardiac stem cells or Sca-1-expressing cardiac progenitor cells in a cardiac fibroblast-conditioned medium in the absence of feeder cells; thereby obtaining a purified isolated cell population enriched in cardiac stem cells or cardiac progenitor cells.