Patent ID: 9193761
Filing Date: 2015-11-24
Classification: C07K

Abstract:
1. A method for purifying a human granulocyte-colony stimulating factor (hG-CSF) from a recombinant Escherichia coli ( E. coli ) in a high yield, comprising the steps of: (a) culturing an hG-CSF-expressing recombinant (b) separating an hG-CSF-containing supernatant from the first cell pellet obtained in step (a); (c) adjusting a pH of the supernatant obtained in step (b) with an acid and separating the resulting precipitate by filtration; (d) subjecting a first filtrate obtained in step (c) to cation exchange chromatography to five a first eluate; (e) subjecting the first eluate to hydrophobic-interaction chromatography to give a second eluate; and (f) subjecting the second eluate to anion exchange chromatography to give a fraction containing hG-CSF.