Patent ID: 6610487
Filing Date: 2003-08-26
Classification: C12Q

Abstract:
A method for objectively distinguishing the presence of non-pathogenic fungi from a Didymella bryoniae fungal infection in a whole plant, plant extract, or plant seed by using cycles of a polymerase chain reaction, said method comprising the steps of:(a) removing a sample of leaf, stem, or seed from a plant to be tested; (b) extracting genomic DNA from said sample to use as a DNA template; (c) replicating a desired region in the DNA template by binding a specific oligonucleotide primer set comprising SEQ ID No. 13 and SEQ ID NO. 14 to said DNA template in a complimentary fashion, said primer set designed to give rise to a desired PCR product having a size different from the size of a product produced from amplifying undesired genomic DNA; (d) amplifying said DNA template by conducting the polymerase chain reaction from about 25 cycles to about 35 cycles to produce a PCR product; and, (e) examining said PCR product to identify Didymella bryoniae or nonpathogenic fungal infection present in said sample.