Patent ID: 6686147
Filing Date: 2004-02-03
Classification: C12Q

Abstract:
A method of diagnosing lung, cancer, comprising:contacting a biological sample comprising lung, cells from a subject with a probe that hybridizes under high-stringency hybridization conditions to a nucleic acid molecule consisting of the nucleotide sequence of SEQ ID NO:4, or a full-length complement thereof, wherein the high-stringency hybridization conditions are hybridization at 65Â° C. in hybridization buffer (3.5Ã—SSC, 0.02% Ficoll, 0.02% polyvinyl pyrrolidone, 0.02% Bovine Serum Albumin, 2.5 mM NaH2PO4(pH7), 0.5% SDS, 2 mM EDTA) and wherein SSC is 0.15M sodium chloride/0.015M sodium citrate, pH7; SDS is sodium dodecyl sulphate; and EDTA is ethylenediaminetetracetic acid, and determining expression of the nucleic acid molecule or its full-length complement in the sample by measuring hybridization of the probe to the nucleic acid molecule or its full-length complement, wherein the expression of the nucleic acid molecule or its full-length complement is diagnostic for lung cancer in the sample.