Patent ID: 6531283
Filing Date: 2003-03-11
Classification: C12Q

Abstract:
A method for detecting one or more analytes, the method comprising(a) bringing into contact one or more analyte samples and one or more analyte capture agents, wherein each analyte capture agent interacts with an analyte directly or indirectly, wherein at least one analyte, if present in the analyte sample, interacts with at least one analyte capture agent, incubating the analyte samples and the analyte capture agents under conditions that promote interaction of the analyte capture agents and analytes, (b) prior to, simultaneous with, or following step (a), bringing into contact at least one of the analyte capture agents and one or more reporter binding primers, wherein each reporter binding primer comprises a specific binding molecule and a rolling circle replication primer, wherein each specific binding molecule interacts with an analyte capture agent directly or indirectly, and incubating the analyte capture agents and the reporter binding primers under conditions that promote interaction of the specific binding molecules and analyte capture agents, (c) prior to, simultaneous with, or following steps (a) or (b), bringing into contact the reporter binding primers and one or more amplification target circles, wherein the amplification target circles each comprise a single-stranded, circular DNA molecule comprising a primer complement portion, wherein the primer complement portion is complementary to at least one of the rolling circle replication primers, and incubating the reporter binding primers and amplification target circles under conditions that promote hybridization between the amplification target circles and the rolling circle replication primers, (d) following step (c) and prior to, simultaneous with, or following steps (a) or (b), incubating the reporter binding primers and amplification target circles under conditions that promote replication of the amplification target circles, wherein replication of the amplification target circles results in the formation of tandem sequence DNA, wherein detection of tandem sequence DNA indicates the presence of the corresponding analytes.