Patent ID: 6074836
Filing Date: 2000-06-13
Classification: C07K,G01N

Abstract:
A method of detecting in a sample, transduced human cells that have been introduced into a human patient, said sample suspected of containing transduced cells and non-transduced host cells, said method comprising:(a) introducing into said patient, a set of human cells which have been transduced with a nucleic acid which encodes and expresses modified cell surface receptor (""marker""), wherein said marker is modified with respect to a counterpart unmodified cell surface receptor that (i) is a human transmembrane protein, and (ii) binds with a ligand and transduces a signal, and wherein said modified cell surface receptor is altered relative to said counterpart unmodified cell surface receptor such that it binds with said ligand but cannot transduce said signal as a result of said ligand binding;(b) obtaining a sample from a patient treated according to step (a), wherein said sample is suspected of containing transduced cells and non-transduced cells, and wherein said non-transduced host cells present in said sample normally do not express said unmodified cell surface receptor; and(c) detecting within said sample, the presence or absence of said marker on the cell surface of said transduced cells, to thereby detect said transduced cells in said sample.