Patent ID: 6384301
Filing Date: 2002-05-07
Classification: C12N

Abstract:
A method of making germline-transformed soybean plants using Agrobacterium mediation, the method comprising:(a) initiating the germination of a soybean seed; (b) isolating the embryonic axis including the embryonic meristem from the soybean seed to prepare an explant; (c) wounding the explant; (d) exposing the explant to a disarmed Agrobacterium vector comprising a heterologous genetic construct comprising a selectable marker gene wherein the heterologous genetic construct is transferred into at least one cell in the explant; (e) culturing the explant in the presence of a selection agent in a manner allowing identification of soybean cells of the explant to which the heterologous genetic construct has been transferred; (f) inducing formation of one or more shoots from the explant, the shoot comprising germline transformed cells; (g) cultivating the shoot into a whole fertile mature soybean plant.