Patent ID: 7728270
Filing Date: 2010-06-01
Classification: G02B

Abstract:
1. A microscopy method for generating an image of an image field located in a predetermined depth of the specimen to be examined, comprising a plurality of illumination steps in which one portion at a time of the image field is illuminated with a focused bundle of illuminating rays, which bundle, by interacting with the specimen, generates specimen radiation, detection steps in which the generated specimen radiation is detected, and an analyzing step in which the image is generated based on the detected specimen radiation, characterized in that during each illumination step, a first detection step and a second detection step are carried out, with intrafocally and extrafocally generated specimen radiation being detected in the first detection step and with a portion of the intrafocally generated specimen radiation that is smaller than that generated in the first detection step as well as extrafocally generated specimen radiation being detected in the second detection step, and in that, in the analyzing step, the specimen radiation detected in the second detection step is subtracted from the specimen radiation detected in the first detection step to provide a result having less extrafocal radiation than the extrafocal portion of the specimen radiation detected in the first detection step.