Patent ID: 6541216
Filing Date: 2003-04-01
Classification: C12Q,Y10S

Abstract:
A single-use disposable test strip apparatus for detecting 3-hydroxybutyric acid in a liquid sample, the apparatus comprising:a substrate, at least one electrode positioned on the substrate, and a single-use reagent in communication with the at least one electrode, the reagent being formed to dissolve in the liquid sample and to generate an electrical output signal indicative of the level of 3-hydroxybutyric acid in the sample, said reagent comprising a hexacyanoferrate (III) salt, a catalytic amount of a first enzyme operative to catalyze the oxidation of 3-hydroxybutyric acid in the sample, a cofactor corresponding to said first enzyme, and a catalytic amount of a second enzyme operative to catalyze the electrochemical oxidation of a reduced form of the cofactor.