Patent ID: 6071707
Filing Date: 2000-06-06
Classification: C07K,G01N

Abstract:
A method for identifying a phosphotyrosine mimic comprising the steps of:(a) providing a test compound and a phosphotyrosine containing substance;(b) contacting the compound and the phosphotyrosine containing substance with an anti-phosphotyrosine antibody; and(c) detecting by immunoassay the ability of the test compound to inhibit the binding of the anti-phosphotyrosine antibody to the phosphotyrosine-containing substance;wherein the phosphotyrosine containing substance and the test compound are capable of binding an anti-phosphotyrosine ant-body;and wherein the identified phosphotyrosine mimic is a phenyl carboxylic acid or a functional group protected derivative thereof, each being optionally substituted with a member selected from the group consisting of halogen, branched or unbranched C.sub.1-5 alkyl, C.sub.1-5 alkoxy, C.sub.2-5 alkenyl, C.sub.2-5 alkynyl, cyano, nitro, amino, trifluoromethyl, trifluoromethoxy and hydroxy; wherein any one of the foregoing alkyl, alkoxy, alkenyl or alkynyl groups is unsubstituted or substituted with cyano, nitro, amino, trifluoromethyl, trifluoromethoxy or hydroxy and wherein any unsubstituted methylene may be replaced with O, S or C.dbd.O.