Patent ID: 9012216
Filing Date: 2015-04-21
Classification: G01N

Abstract:
1. A method for determining efficacy of a cyclodextrin therapy in a subject afflicted with a disorder involving the accumulation of one or more oxysterols, comprising: (a) obtaining at least one first body fluid sample from the subject prior to administering a cyclodextrin to the subject; (b) administering the cyclodextrin to the subject; (c) obtaining at least one second body fluid sample from the subject after the administering the cyclodextrin; (d) subjecting the at least one first body fluid sample to a first chromatography-mass spectroscopy analysis to determine concentration of 24-hydroxycholesterol; (e) subjecting the at least one second body fluid sample to a second chromatography-mass spectroscopy analysis to determine concentration of 24-hydroxycholesterol; and (f) determining magnitude of difference between the 24-hydroxycholesterol concentration of the at least one first body fluid sample and the at least one second body fluid sample, whereby an increase or stabilization of 24-hydroxycholesterol concentration in the second sample compared to the first sample indicates efficacy of the cyclodextrin therapy.