Patent ID: 9212231
Filing Date: 2015-12-15
Classification: A61K,A61P,C07K

Abstract:
1. A TRAIL R2-specific recombinant multimeric scaffold comprising at least two Tn3 monomer scaffolds, wherein (a) each Tn3 monomer scaffold comprises seven beta strands designated A, B, C, D, E, F, and G, and six loop regions designated AB, BC, CD, DE, EF, and FG, wherein the AB loop comprises SEQ ID NO: 35, the BC loop comprises a sequence selected from the group consisting of SEQ ID NOs: 97, 98, or 168, the CD loop comprises SEQ ID NO: 37, the DE loop comprises a sequence selected from the group consisting of SEQ ID NOs: 102, 103, and 179, the EF loop comprises SEQ ID NO: 39, and FG loop comprises a sequence selected from the groups consisting of SEQ ID NOs: 106, 108, 109, 169, and 170, (b) the Tn3 monomer scaffold comprises at least one disulfide bond to link any two of the seven beta strand domains, (c) the Tn3 monomer scaffolds are connected in tandem, and (d) the recombinant multimeric scaffold specifically binds to TRAIL R2.