Patent ID: 7964356
Filing Date: 2011-06-21
Classification: C12N,C12Q

Abstract:
1. A method for detecting a target in a tissue or cell sample comprising: a) obtaining a tissue or cell sample and preparing said sample into a plurality of tissue sections or cell preparations; b) contacting a tissue section or a cell preparation with a solution comprising a slow off-rate aptamer to a target, wherein said aptamer comprises at least one C-5 modified pyrimidine, and wherein said aptamer is a product of a SELEX process that comprises a slow off-rate enrichment process; c) comparing the results obtained in step b) with a negative control prepared by reacting a second tissue section or cell preparation with a solution as in step b) that lacks said slow off-rate aptamer; and d) detecting said slow off-rate aptamer as an indication of the presence or absence of said target.