Patent ID: 7280203
Filing Date: 2007-10-09
Classification: G01J,G01N,G02B

Abstract:
1. In a scanning microscope a method for separating fluorescence spectra of dyes present in a specimen, comprising the steps of: acquiring a spectral scan of the specimen and thereby ascertaining an acquired mixed fluorescence spectrum of all the dyes present in the specimen; storing individual fluorescence spectra corresponding to the respective dyes present in the specimen in a computer system associated with the scanning microscope; spectrally deconvoluting the acquired mixed fluorescence spectrum to obtain spectrally deconvoluted measured individual spectra and comparing the measured individual spectra to the individual fluorescence spectra corresponding to the respective dyes; generating an alarm signal if comparing the measured individual spectra to the individual fluorescence spectra is unsatisfactory with regard to a safeguard function based on a threshold value; linearly deconvoluting the acquired mixed fluorescence spectrum if no alarm is generated and presenting a result of the deconvoluting steps on a display.