Patent ID: 6919436
Filing Date: 2005-07-19
Classification: B01D,B01J,C07K

Abstract:
1. A method for the isolation of proteins from a solution containing one or more of proteins, comprising the following operations: a) contacting a solution containing one or more proteins having a pH in the range of 2.5 to 5.5 and a total salt content corresponding to an ionic strength of at the most 2.0 with a solid phase matrix comprising a functionalised matrix backbone carrying a plurality of functional groups of the following formula wherein M designates the matrix backbone and SP1-X-A-SP2-ACID designates a ligand -L; SP1 designates a spacer which is not a divinyl sulfone derived spacer; X designates —O—, —S—, or —NH—; A designates a mono- or bicyclic optionally substituted aromatic or heteroaromatic moiety; SP2 designates an optional spacer; and ACID designates an acidic group; with the proviso that the molecular weight of the ligand -L is at the most 500 Dalton; whereby at least a part of the proteins becomes bound to the solid phase matrix; b) separating the solid phase matrix having proteins bound thereto from the solution; c) optionally washing the solid phase matrix; and d) contacting the solid phase matrix with an eluent in order to liberate one or more of the proteins from the solid phase matrix, wherein the eluent used comprises less than 10% (v/v) of organic solvents.