Patent ID: 9149473
Filing Date: 2015-10-06
Classification: A61K,C12P,C12Q,H01J,Y02A

Abstract:
1. A method comprising: a) amplifying at least one pathogen genome from a sample suspected of comprising at least one pathogen genome and at least one background genome using at least one targeted whole genome amplification primer pair, thereby elevating the quantity of nucleic acid representing said at least one pathogen genome relative to the quantity of nucleic acid representing said at least one background genome, wherein said plurality of targeted whole genome amplification primers is selected by: b) producing one or more amplification products representing bioagent identifying amplicons from said amplified pathogen genome using said at least one primer pair; c) measuring molecular masses of said amplification products by mass spectrometry; d) calculating base compositions of said amplification products from said molecular masses without sequencing said amplification products; and e) comparing said base compositions with a database comprising base compositions of bioagent identifying amplicons of pathogens produced with said at least one primer pair, thereby identifying said pathogen in said sample.