Patent ID: 7713690
Filing Date: 2010-05-11
Classification: C12Q,G01N

Abstract:
1. A method for chemically identifying a toxic substance in a sample using a luminescent biological agent, said method comprising the steps of: preparing the luminescent biological agent for use in conjunction with chromatography paper; obtaining a sufficient volume of a sample suspected to contain toxic substances to provide a test sample; separating toxic substances of the test sample on a separation phase matrix to provide a first set of serial aliquot volumes; exposing said first set of serial aliquot volumes to said luminescent biological agent by spotting said serial aliquot volumes in an array or in a linear fashion on a sheet of chromatography paper and spraying said sheet with a suspension of luminescent biological agent; identifying the presence of toxic substances in said first set of serial aliquot volumes by zones of luminescent inhibition on said chromatography paper; obtaining a second volume of the sample to form a second test sample; separating the toxic substances of the second test sample on another separation phase matrix to provide a second set of serial aliquot volumes; and determining the chemical identity of a separated toxic substance observed at areas of luminescent inhibition with said first set of serial aliquot volumes by analyzing a corresponding inhibition region with said second set of serial aliquot volumes.