Patent ID: 8541206
Filing Date: 2013-09-24
Classification: C12P,C12Q

Abstract:
1. A method for the differential amplification of a nucleic acid by polymerization chain reaction (PCR), wherein the method consists essentially of: (a) providing a nucleic acid mixture comprising a parental DNA and a GC allele of the parental DNA; (b) carrying out PCR in the nucleic acid mixture in a reaction medium containing deoxyinosine (dITP), or in a reaction medium containing deoxy 2,6-diaminopurine triphosphate (dDTP), or in a reaction medium containing dITP and dDTP, wherein Guanine bases (G) are converted into Inosine bases (I) and Adenosine bases (A) are converted into 2,6-diaminopurine bases (D); and (c) subjecting nucleic acids of (b) to PCR using a denaturation temperature 1-10° C. lower than the lowest denaturation temperature (Tp) that allows amplification of the parental DNA to thereby selectively amplify the GC allele in the nucleic acid mixture.