Patent ID: 7462457
Filing Date: 2008-12-09
Classification: A01K,C07K,C12N,Y02A

Abstract:
1. A method for identifying constitutively activating mutations in an orphan receptor or an ion channel, comprising: (a) identifying small or medium side-chain amino acid residues of a candidate orphan receptor that are located in or proximate to a transmembrane segment of the orphan receptor; (b) providing a library of coding sequences for potentially activating mutations of the candidate orphan receptor or ion channel, wherein said library is generated by replacing only coding sequences for the identified small or medium side-chain amino acids with coding sequences for large side-chain amino acids, wherein the mutation is conservative with respect to charge; (c) expressing the library in mammalian host cells; (d) measuring an activity of the encoded receptor or ion channel in the mammalian host cells; and (e) identifying those coding sequence(s) which encoded activated receptors or ion channels.