Patent ID: 8118986
Filing Date: 2012-02-21
Classification: G01N

Abstract:
1. A method for separating and determining the hydrodynamic radius of the constituents of an admixture M, which comprises the following steps: (A) the constituents of the admixture M are separated by using the technique of capillary electrophoresis, and by leaving them within the capillary; (B) at one of the ends of the capillary obtained in this manner, containing, in different zones, the constituents separated in step (A), a detectable marker is injected in the region of a detection device which is placed at the side of the other end of the capillary; (C) a positive hydrostatic pressure difference is induced between the end of the capillary via which the marker of step (B) was introduced and the other end of the capillary, whereby a displacement of the different species present in the capillary is induced, associated with a phenomenon of Taylor dispersion, and the various constituents separated during step (A) and finally the marker introduced during step (B) are allowed to migrate in front of the detector at the outlet of the capillary; and (D) by analysing the Taylor dispersion obtained, the hydrodynamic radius is determined for each of the constituents, based on the detection time of the marker and the elution profile of each of the constituents.