Patent ID: 9149441
Filing Date: 2015-10-06
Classification: A61K,A61P,C12N

Abstract:
1. A method for forming nanospheres containing bioactive material, comprising: a) dissolving a polymer matrix in an aqueous medium in a first vessel; b) contacting said dissolved polymer matrix with a crosslinking agent to crosslink said polymer matrix and said crosslinking agent; c) neutralizing with sodium bisulfate any excess crosslinking agent from said crosslinked polymer matrix-crosslinking agent after crosslinking; d) solubilizing in a second vessel a bioactive material in an aqueous solution; e) combining said solubilized bioactive material of step (d) together with said neutralized crosslinked polymer matrix in solution of step (c); and, f) spray drying the result of step (e) to produce nanospheres, whereby bioactivity of said biomaterial is retained upon cellular uptake.