Patent ID: 6406863
Filing Date: 2002-06-18
Classification: C07K

Abstract:
A method for selecting tester proteins capable of binding to a target peptide or protein, the method comprising:expressing a library of tester fusion proteins in yeast cells, each tester fusion protein comprising either an activation domain or a DNA binding domain of a transcription activator and a tester protein having a diversity of at least 1Ã—107 within the library, the tester protein comprising a first polypeptide subunit whose sequence varies within the library, a second polypeptide subunit whose sequence varies within the library independently of the first polypeptide, and a linker peptide which links the first and second polypeptide subunits; expressing a target fusion protein in the yeast cells expressing the tester fusion proteins, the target fusion protein comprising either the DNA binding domain or the activation domain of the transcription activator which is not comprised in the tester fusion proteins, and a target peptide or protein; and selecting those yeast cells in which a reporter gene is expressed, the expression of the reporter gene being activated by a reconstituted transcriptional activator formed by binding of the tester fusion protein to the target fusion protein.