Patent ID: 9193961
Filing Date: 2015-11-24
Classification: C12N

Abstract:
1. An isolated or purified polynucleotide encoding an enzyme having carboxylesterase [E.C. 3.1.1.1] activity, wherein the polynucleotide is fused to a heterologous promoter and wherein the polynucleotide is selected from the group consisting of (a) a polynucleotide encoding an amino acid sequence as set forth SEQ ID NO:10; (b) a polynucleotide comprising a nucleotide sequence set forth in SEQ ID NO:9; (c) a polynucleotide comprising a nucleotide sequence encoding a fragment of an enzyme encoded by a polynucleotide of (a) or (b), and wherein said fragment has a Sm-Xaa-Ser-Xaa-Gly (SEQ ID NO:13) motif, a catalytic triad composed of amino acids Ser-Glu-His or Ser-Asp-His, and a GGGX (SEQ ID NO:15) motif; (d) a polynucleotide at least 70% identical to a polynucleotide encoding an enzyme as set forth in one of SEQ ID NO:10, wherein the enzyme has a Sm-Xaa-Ser-Xaa-Gly (SEQ ID NO:13) motif, a catalytic triad composed of amino acids Ser-Glu-His or Ser-Asp-His, and a GGGX (SEQ ID NO:15) motif; and (e) a polynucleotide comprising a nucleotide sequence degenerate to the polynucleotide of (d).