Patent ID: 7985600
Filing Date: 2011-07-26
Classification: G01N

Abstract:
1. A method for determining the binding constant of interacting molecular species and of the concentration of a ligand in a solution comprising the step of: measuring the intensity of the light reflected by a flat surface comprising at least one perfluoropolymer, at least one receptor that is adsorbed to the surface of the perfluoropolymer or chemically bound to the surface of the perfluoropolymer, and at least one ligand molecule that interacts with the receptor by the steps of: contacting a surface of the perfluoropolymer with an aqueous solution containing from 1 nanogram/ml to 10 milligrams/ml of the receptor to form a first solution; optionally measuring the light intensity reflected from the interface between the aqueous solution of the receptor and the surface of the perfluoropolymer in the first solution; optionally contacting a surface of the perfluoropolymer with other aqueous or non-aqueous solutions of the receptor; adding a predetermined volume of an aqueous solution of a ligand having a predetermined concentration to the first solution to obtain a second solution, wherein the ligand concentration is [T repeating the adding of a predetermined volume of an aqueous solution of a ligand step; measuring after each adding of a predetermined volume of an aqueous solution of a ligand step the light intensity I reflected from the interface between the aqueous solution of the ligand and the surface of the perfluoropolymer in the second solution; and correlating the reflected light intensity data I as a function of the ligand concentration [T wherein I c is a factor taking into account the surface roughness and is 1 only in case of a flat surface, I v is the direction of the light polarization the incidence plane, R