Patent ID: 6096508
Filing Date: 2000-08-01
Classification: C12Q,G01N,Y10S

Abstract:
In a method of detecting a target material in a sample immobilized on a solid phase support using a detector molecule, wherein the detector molecule binds to the target material, wherein said detector molecule has a heterologous moiety attached thereto, wherein said heterologous moiety attached to the detector molecule is detected by a signaling moiety, wherein the signaling moiety binds to the heterologous moiety, and wherein said method comprises a step of washing excess non-specifically bound signaling moiety with a wash solution from said solid phase support, the improvement comprising employing as said wash solution a composition comprising free heterologous moiety, wherein the heterologous moiety attached to the detector molecule is selected from the group consisting of biotin, biotin derivatives and biotin analogs; wherein the free heterologous moiety is selected from the group consisting of biotin, biotin derivatives and biotin analogs and wherein the signaling moiety is selected from the group consisting of modified streptavidin and modified avidin.