Patent ID: 8933218
Filing Date: 2015-01-13
Classification: A61K,A61P,Y10S

Abstract:
1. A process of conjugating an isolated staphylococcal poly-N-acetylglucosamine (PNAG) which is less than 40% N-acetylated to a carrier protein comprising the steps of: a) activating the PNAG by adding a linker comprising a maleimide group to form an activated PNAG, wherein the weight/weight ratio of the PNAG to the linker comprising the maleimide group is 1:2-2:1; b) activating the carrier protein by adding a linker with a spacer length of 10-25 Angstroms, wherein the linker comprises a sulphydryl group, to form an activated carrier protein, wherein the weight/weight ratio of the carrier protein to the linker comprising the sulphydryl group is 15:1 to 5:1; and c) reacting the activated PNAG and the activated carrier protein to form a PNAG-carrier protein conjugate, wherein the weight/weight ratio of the activated PNAG to the activated carrier protein is 4:1-1:4.