Patent ID: 6485900
Filing Date: 2002-11-26
Classification: A61K,C07K,C12N,Y10S

Abstract:
A method for determining the presence of antibodies to LAV/HTLV-III in a test sample suspected of containing said antibodies, said method comprising:combining the test sample with a peptide immunoreactive with antibodies to LAV/HTLV-III having from eleven amino acids to about thirty-five amino acids, wherein the amino acid sequence of said peptide is a contiguous LAV/HTLV-III sequence and includes at least eleven amino acids from the following sequence: Gly-Ile-Trp-Gly-Cys-Ser-Gly-Lys-Leu-Ile-Cys and peptide analogues thereof having a sequence of a LAV/HTLV-III strain and having immunoreactivity to antibodies to LAV/HTLV-III; incubating for a sufficient time for immune complex formation to occur between said peptide and said antibodies to LAV/HTLV-III; and determining the formation of immune complex and thereby detecting the presence or absence of said antibodies.