Patent ID: 7935497
Filing Date: 2011-05-03
Classification: G01N

Abstract:
1. A method of detecting a protease in a sample comprising the steps of: (i) providing an unlabelled protein analyte degradable by the protease; (ii) contacting the analyte with the sample; (iii) contacting the analyte with a specific labelling component such that the labelling component specifically binds to the analyte, wherein the labelling component is associated with a label; (iv) capturing the analyte with a specific capture component such that, if intact, the analyte acts as a link between the labelling component and the capture component; and (v) detecting the presence of the label associated with the capture component, wherein the absence of a detectable label or detection of a reduced label intensity at the location at which the capture component is immobilised is indicative of the presence of a protease in the sample.