Patent ID: 8815507
Filing Date: 2014-08-26
Classification: C07H,C12N,C12Q

Abstract:
1. A cooperative hybridization system comprising: first and second target oligonucleotide strands, said first and second target oligonucleotide strands being either identical or independent sequences; a nucleic acid intermediary complex comprising a substrate strand and a protector strand, a binding region of the protector strand being bound to said substrate strand, the substrate strand having a plurality of independent binding domain regions that are complementary to the first and second target oligonucleotide strands for binding to said first and second target oligonucleotide strands, each of the independent binding domain regions being formed from a plurality of individual domains, and said independent binding domain regions being adjacent each other such that, when said nucleic acid intermediary complex hybridizes to said first and second target oligonucleotide strands, said first and second target oligonucleotide strands are co-localized on said substrate strand, wherein the length of the binding region of the protector strand is less than the sum of the lengths of the binding regions of the first and second target oligonucleotide strands, and wherein the binding regions of the first and second target oligonucleotide strands are fully complementary with the independent binding domain regions of the substrate strand, and wherein the hybridization of one of the first and second target oligonucleotide strands to the substrate strand of said nucleic acid intermediary complex only partially and reversibly displaces the protector strand from said substrate strand; and wherein the hybridization of both first and second target oligonucleotide strands to the substrate strand irreversibly releases said protector strand from said substrate strand, and produces a dual-hybridized product including both first and second target oligonucleotide strands and substrate strand.