Patent ID: 8357680
Filing Date: 2013-01-22
Classification: A61K,A61P,C07D,C12Q,G01N

Abstract:
1. A method for screening a hypoglycemic compound, comprising using a compound represented by the following general formula (I) or a pharmaceutically acceptable salt thereof (hereinafter referred to as “said compound”) and a trimeric GTP-binding protein β subunit (hereinafter referred to as “said protein”), by measuring an inhibitory activity of a test substance against binding of the said compound to the said protein: wherein in the general formula (I), A and B may be the same or different and independently represent an optionally substituted aromatic ring, an optionally substituted heterocyclic ring, or an optionally substituted aliphatic ring; R —X— and —Y— may be the same or different and independently represent a hydrogen atom, —O—, —NR —W— represents an optionally substituted alkyl chain having 1 to 20 carbon atoms, and 1 to 10 carbon atoms in the alkyl chain may be replaced by —O—, —NR Q represents a hydrogen atom, biotin, a fluorophore, a chromophore, a chemiluminescent functional group, an enzyme, a solid phase, a diazo group, or an azido group; one or more atoms in the formula may be (a) radioisotope(s); with the proviso that: i) in the optionally substituted groups, each substituent is selected from the group consisting of halogen atoms, a hydroxyl group, alkyl groups, mercapto groups, alkoxy groups, alkylthio groups, alkylsulfonyl groups, acyl groups, acyloxy groups, amino groups, alkylamino groups, a carboxyl group, alkoxycarbonyl groups, carbamoyl groups, a nitro group, a cyano group, a trifluoromethyl group, aryl groups, heteroaryl groups, diazo groups, and azido groups, and the substituent may be labeled with biotin, a fluorophore, a chromophore, a chemiluminescent moiety, or an enzyme; ii) when —X— is a hydrogen atom, —W—, —Y—, and Q do not exist; and iii) when —Y— is a hydrogen atom, Q does not exist.