Patent ID: 7462758
Filing Date: 2008-12-09
Classification: C12N

Abstract:
1. A method for integration of a DNA sequence into a plastid DNA of a plant or plant cell, said method comprising: a) combining a transformation construct comprising an insertion sequence with at least one enzyme suitable for directed induction of DNA double-strand breaks at a recognition sequence of the plastid DNA in at least one plastid of a plant or plant cell, wherein the plastid DNA comprises at least one recognition sequence for the directed induction of DNA double-strand breaks; b) inducing DNA double-strand breaks at the recognition sequence; and c) inserting the insertion sequence into the recognition sequence of the plastid DNA at the DNA double-strand breaks, wherein functionality of the recognition sequence for the directed induction is deactivated and said recognition sequence is no longer capable of being cleaved by the at least one enzyme; wherein the at least one enzyme is expressed in the plastid of a plant or plant cell or expressed in the nucleus and transported to the plastid of a plant or plant cell.