Patent ID: 8673554
Filing Date: 2014-03-18
Classification: G01N,Y10T

Abstract:
1. A method for measuring at least one intracellular event and a cell-surface associated analyte in a single population of living cells wherein said at least one intracellular event and said cell-surface associated analyte are each components of a concerted biochemical process operating in said cells, the method comprising the steps of: a) providing a sample containing a single population of living cells; b) contacting at least one living cell in said single population of cells with a test agent causing said at least one cell to produce a cell-surface associated analyte; c) measuring a change in a physical property in said at least one cell in step b) as a measure of at least one intracellular event; d) washing the cells to remove extracellular fluids; e) measuring the presence, amount or activity of said produced cell-surface associated analyte; and f) correlating the measured change in said at least one intracellular event in said at least one cell with the measured presence, amount or activity of said cell-surface associated analyte.