Patent ID: 6197588
Filing Date: 2001-03-06
Classification: C07K,C12N

Abstract:
A method of targeting a protein or polypeptide to the inner envelope membrane of a plastid of a plant cell, comprisingintroducing a chimaeric gene comprising a promoter, a coding sequence for a chimaeric protein, and a terminator sequence, into a plant cell;wherein the promoter is capable of expressing the chimaeric protein in the plant cell; andwherein the coding sequence comprises a first nucleotide sequence encoding a first polypeptide consisting essentially of an amino terminal hydrophobic region of a mature phosphate translocator protein, said mature phosphate translocator protein residing in the inner envelope membrane of a plastid; and a second nucleotide sequence encoding a second heterologous protein or polypeptide which is to be targeted to the inner envelope membrane of a plastid.