Patent ID: 7498176
Filing Date: 2009-03-03
Classification: B01J,B01L,B82Y,C07B,C40B,Y02P,Y10T

Abstract:
1. A method for preparing a microarray having a hydrophobic barrier defining a plurality of subarrays on the microarray, the microarray constructed by a light-directed maskless array synthesizer instrument, the method comprising the steps of: a) selecting at least one probe set comprising probes of interest; b) synthesizing the probe sets selected in step a) on a microarray slide to provide the plurality of subarrays using the light-directed maskless array synthesizer instrument; c) depositing between each of the subarrays synthesized in step b) a hydrophobic group-bearing phosphoramidite to provide a hydrophobic barrier which surrounds each subarray, wherein the deposit of the hydrophobic barrier is made by using the same light-directed maskless array synthesizer instrument used in step b); and d) inhibiting fluid communication between each of the subarrays on the microarray.