Patent ID: 8951745
Filing Date: 2015-02-10
Classification: C07K,C12Q,G01N

Abstract:
1. A method of determining whether a binding partner of a Futrin 3 polypeptide affects Wnt signaling activity, the method comprising: (a) providing a mammalian cell in culture comprising the Futrin 3 polypeptide and a Wnt inducible reporter, wherein the Futrin 3 polypeptide comprises the amino acid sequence of SEQ ID NO:29, the amino acid sequence of amino acids 21-262 of SEQ ID NO:29, the amino acid sequence of amino acids 1-149 of SEQ ID NO:29, or the amino acid sequence of amino acids 21-149 of SEQ ID NO:29, wherein the Futrin 3 polypeptide is able to promote Wnt signaling; and (b) detecting the level of reporter expression in the cell in the presence and absence of the binding partner, wherein a change in the level of reporter expression indicates that the binding partner affects the Wnt signaling.