Patent ID: 6630327
Filing Date: 2003-10-07
Classification: A61K,C07K,C12N,G01N

Abstract:
A method for producing an antibody or antigen binding fragment thereof specific for P-glycoprotein in a biochemical conformation adopted in the presence of Pgp-mediated transport substrates or ATP depleting agents, the method comprising the steps of introducing a cell expressing a heterologous, mutant P-glycoprotein into an animal syngeneic with the species from which the cell was derived, wherein the heterologous, mutant P-glycoprotein is in a biochemical conformation adopted in the presence of Pgp-mediated transport substrates or ATP depleting agents, and wherein the heterologous, mutant P-glycoprotein has an amino acid sequence encoded by human MDR1 except that the sequence comprises an amino acid residue at positions 433 and 1076 of the Pgp amino acid sequence that is independently changed to methionine, and producing immune cells in the animal expressing an antibody specific for P-glycoprotein in said biochemical conformation.