Patent ID: 7964340
Filing Date: 2011-06-21
Classification: C12Q,G01N

Abstract:
1. A method for detecting an amine, enzyme, or enzyme inhibitor within a test sample, the method comprising: i) contacting an incubation mixture that comprises the test sample that may contain an amine, enzyme, or enzyme inhibitor and further comprises a plurality of reactive complexes that each comprises a substrate joined to a reporter and a specific binding member, said substrate being cleavable by a hydrolytic enzyme to release said reporter with a chromatographic medium, said chromatographic medium defining an enzyme detection zone within which is immobilized a receptive material that has an affinity for said specific binding member for generating an enzyme detection signal wherein the presence or quantity of a hydrolytic enzyme is determinable from said enzyme detection signal, and an amine detection zone positioned downstream from said enzyme detection zone, wherein a chemichromic dye is contained within said amine detection zone, said chemichromic dye being capable of undergoing a color change in the presence of an amine, wherein the presence or quantity of an amine is determinable from said color change; ii) determining the presence or quantity of an enzyme or enzyme inhibitor from said enzyme detection signal; and iii) determining the presence or quantity of an amine from said amine detection signal.