Patent ID: 7226785
Filing Date: 2007-06-05
Classification: A61K,A61P,C12N

Abstract:
1. A method of optimizing an oligonucleotide for in vitro targeted chromosomal sequence alteration, comprising: (a) Providing a first oligonucleotide and a second oligonucleotide for alteration of a targeted nucleic acid sequence, (b) combining the targeted nucleic acid with said first or said second oligonucleotide in the presence of cellular repair proteins; (c) combining the targeted nucleic acid with the other of said first or second oligonucleotide in the presence of cellular repair proteins; (d) quantifying the percentage of target molecules that undergo a sequence alteration event; (e) comparing the efficiency of alteration of said targeted nucleic acid sequence by said first oligonucleotide with the efficiency of alteration of the same targeted nucleic acid sequence by said second oligonucleotide; and (f) repeating steps (a) through (e) two or more times to optimize said first oligonucleotide for in vitro targeted chromosomal sequence alteration.