Patent ID: 7482145
Filing Date: 2009-01-27
Classification: G01N

Abstract:
1. A method to produce a conjugate of an alkaline phosphatase enzyme and an antibody or functional fragment thereof which binds to a target molecule in a detection assay, wherein the alkaline phosphatase enzyme comprises at least one glycosylation site that is recognized in a transformed yeast expression host, the method comprising the steps of: (a) providing a glycosylated alkaline phosphatase enzyme comprising the amino acid sequence set forth as SEO ID NO: 1 and obtained by expression in the transformed yeast and isolated therefrom, (b) at least partially deglycosylating the glycosylated enzyme of step (a), (c) isolating the dcglycosylatcd enzyme, and (d) attaching the deglycosylated enzyme of step (c) to the antibody or functional fragment thereof capable of binding to a target molecule in a detection assay.