Patent ID: 8426173
Filing Date: 2013-04-23
Classification: C12N,C12P,C12Y,Y02E

Abstract:
1. A method for the production of 1-butanol comprising: a) providing a recombinant microbial production host cell which is tolerant to 1-butanol, and wherein the recombinant microbial host cell comprises heterologous DNA molecules encoding polypeptides that catalyze each of the following substrate to product conversions: b) seeding the production host of (a) into a fermentation medium comprising at least one fermentable carbon substrate to create a fermentation culture; c) growing the production host in the fermentation culture at a first temperature for a first period of time determined by a change in at least one monitored metabolic parameter of the fermentation culture; d) lowering the temperature of the fermentation culture to a second temperature for a second period of time determined by a change in at least one monitored metabolic parameter of the fermentation culture; e) optionally lowering the temperature of the fermentation culture to a third temperature for a third period of time determined by a change in at least one monitored metabolic parameter of the fermentation culture; wherein steps (c), (d) and (e) are optionally repeated to maintain a desired rate of 1-butanol production.