Patent ID: 6159698
Filing Date: 2000-12-12
Classification: G01N,Y10S,Y10T

Abstract:
An assay method for measuring the amount of mycophenolic acid in a sample suspected of containing said mycophenolic acid and endogenous proteins that form a complex with said mycophenolic acid, said method comprising:(a) combining in an aqueous medium (i) said sample, (ii) at least one assay reagent comprising a signal producing system which produces a detectable signal depending on the amount of said mycophenolic acid in the sample, wherein the signal producing system comprises an exogenous binding partner that specifically binds to mycophenolic acid, and (iii) a releasing agent in an amount effective to release said mycophenolic acid from the complex thereof with said endogenous proteins to provide at least about 90% of said mycophenolic acid in a form free of such complex, and(b) determining the amount of said mycophenolic acid by measuring the signal produced by the signal producing system.