Patent ID: 8030074
Filing Date: 2011-10-04
Classification: A61P,C07K,C12N,C12P

Abstract:
1. A method for producing in an in vitro translation system comprising a cell extract, at least one protein comprising at least one unnatural amino acid, the method comprising: providing the translation system with at least one nucleic acid comprising at least one selector codon, wherein the nucleic acid encodes the at least one protein; providing the translation system with an orthogonal tRNA (O-tRNA), wherein the O-tRNA functions in the translation system and wherein the O-tRNA recognizes the at least one selector codon; providing the translation system with an orthogonal aminoacyl tRNA synthetase (O-RS), wherein the O-RS preferentially aminoacylates the O-tRNA with the at least one unnatural amino acid in the translation system wherein the O-RS is selected from the group consisting of SEQ ID NO: 35-64; and providing the translation system with at least one dipeptide or tripeptide comprising at least one unnatural amino acid, thereby producing in the translation system the at least one protein comprising the at least one unnatural amino acid.