Patent ID: 7186503
Filing Date: 2007-03-06
Classification: A61K,A61P,C12N,C12Y

Abstract:
1. A method for screening for an agent that modulates NF-κB activity comprising the steps of: a) contacting a candidate agent with an isolated human E3 ubiquitin ligase polypeptide comprising SEQ ID NO:16 or a variant of SEQ ID NO:16 that is encoded by the full-length complement of a polynucleotlde that hybridize to a polynucleotide encoding SEQ ID NO:16 under stringent conditions comprising washing at 65° C. in 0.2×SSC containing 0.1% SDS wherein said variant retains the ability to enhance ubiquitination of phosphorylated IκB, under conditions and for a time sufficient to permit interaction between the polypeptide and the candidate agent; and b) determining whether the polypeptide enhances ubiquitination of phoshorylated IκB in the presence of the andidate agent, relative to a predetermined ability of the polypeptide to enhance ubiquitination of phoshorylated IκB in the absence of the candidate agent, and, if so; c) identifying an agent that modulates NF-κB activity.