Patent ID: 8765196
Filing Date: 2014-07-01
Classification: C07D

Abstract:
1. A method for separating and purifying Ginkgolide C from root bark of ginkgo, comprising the steps of: (1) extracting the root bark of ginkgo as a raw material with 50% by weight of ethanol; (2) concentrating the resulting extract under vacuum to remove ethanol; (3) loading the resulting concentrate on a macroporous resin column chromatography; (4) washing the column with 2 folds column volume of pure water to remove impurities, and then eluting the column with 80% to 95% by weight of an ethanol solution; (5) concentrating the eluate under vacuum to dryness to obtain a yellow crude extract; (6) heating the crude extract in water to boiling to form a solution, and then refrigerating the solution; (7) obtaining a supernatant from the refrigerated solution and concentrating the supernatant to ⅕ volume of the initial volume to produce a large number of lactone particles, and then filtering under vacuum with filter paper to obtain a white mixed crude crystal of ginkgolides; (8) dissolving the crude crystal in 95% by weight of ethanol to form a supersaturated solution, refrigerating and crystallizing the supersaturated solution to remove Ginkgolides A and B; (9) concentrating and recrystallizing the resulting solution to obtain a crystal of Ginkgolide C with a content of more than 80%; and (10) recrystallizing the crystal with ethanol several times to obtain a high-purity crystal of Ginkgolide C.