Patent ID: 8263395
Filing Date: 2012-09-11
Classification: C12N

Abstract:
1. A method for producing adenoviruses, which comprises: a) constructing a shuttle plasmid, which comprises a first truncated recombinant adenoviral genome which contains at least one heterologous nucleic acid, b) bringing this shuttle plasmid into contact with a parent plasmid, which comprises a second truncated recombinant adenoviral genome which is complementary to the first genome, making it possible, by means of one homologous recombination step, to produce a final plasmid which comprises a complete recombinant adenoviral genome, and c) excising the complete linear recombinant adenoviral genome from the final plasmid and introducing it into an encapsidating cell line in order to produce the recombinant adenoviruses which incorporate the complete recombinant adenoviral genome, said shuttle plasmid and parent plasmid both comprising an ITR sequence bordered by a restriction site not present on the adenoviral genome.