Patent ID: 7807349
Filing Date: 2010-10-05
Classification: B82Y,G01N

Abstract:
1. A method for monitoring synthesis of one or more proteins which comprises: binding a first label to at least one ribosome or a fragment thereof to form a donor fluorophore; binding a second label to at least one tRNA to form an acceptor fluorophore; detecting electromagnetic radiation signals emitted when the first and second labels are in proximity, wherein the signals are obtained from the donor and acceptor fluorophores forming a fluorescence resonance energy transfer (FRET) pair, with the signals indicating progression of the synthesis of the one or more proteins; and analyzing the detected signals to characterize one or more proteins being synthesized by computing a synthesis signal from the FRET pairs by: interrogating a database compiled from protein sequences transformed to a label sequence by marking each amino acid as “N” or “F” according to whether its synthesis will result in a FRET signal or not.