Patent ID: 7588896
Filing Date: 2009-09-15
Classification: C12Q

Abstract:
1. A method of determining a target polynucleotide comprising; providing a target polynucleotide, a primer set, and a hairpin linker, amplifying the target polynucleotide in a polymerase chain reaction (PCR) using the forward primer and the reverse primer to form a first amplification product; melting the first amplification product; hybridizing the single-stranded stem portion of the forward primer to the complement of the single-stranded stem portion of the second primer to form a self-complementary amplification product; hybridizing the protruding tail of the self-complementary amplification product to the protruding tail of the hairpin linker; ligating the self-complementary amplification product to the hairpin linker to form a loop ligation product; detecting the loop ligation product; and, determining the target polynucleotide.