Patent ID: 6379889
Filing Date: 2002-04-30
Classification: C12Q,Y10S,Y10T

Abstract:
A method for separating nucleic acid samples in a test mixture using denaturing liquid chromatography, the method comprising the steps ofproviding a test mixture comprising a plurality of nucleic acid samples, wherein each sample is labeled with a different spectrally detectable tag that produces a different spectrally resolvable signal; applying the test mixture to a reversed phase support in a denaturing liquid chromatography column; eluting the test mixture under partially denaturing conditions to separate at least one of the nucleic acid samples from the test mixture, the partially, denaturing conditions being selected from at least one or both of temperature-induced or chemical-induced conditions; and detecting the spectrally resolved signals produced by the nucleic acid samples labeled with the different spectrally detectable tags.