Patent ID: 6569659
Filing Date: 2003-05-27
Classification: A23K,A23L,A63B,C12N,C12P,C12Y

Abstract:
An isolated polynucleotide which encodes a polypeptide having phytase activity and hybridizes under high stringency conditions with any of the DNA sequences of SEQ ID NO:21, 25, 27 or 29, or with any of the DNA sequences cloned into plasmid pYES 2.0 present in Escherichia coli DSM 11313, 11842, 11843, or 11844, wherein the high stringency conditions are defined as prehybridization in a solution of 5Ã—SSC, 5Ã—Denhardt's solution, 0.5% SDS and 100 micro-g/ml of denatured sonicated salmon sperm DNA, followed by hybridization in the same solution for 12 hours at approximately 45Â° C., followed by washing twice for 30 minutes in 2Ã—SSC, 0.5% SDS at at least 70Â° C.