Patent ID: 9206461
Filing Date: 2015-12-08
Classification: C12Q,C25D,G01N,Y10T

Abstract:
1. A sensor for detecting a microorganism based on a state of capturing said microorganism in a mold, said sensor comprising (A) a detection unit including a detection electrode and a polymer layer that is disposed on a roughened surface of said detection electrode and includes a mold having a three-dimensional structure complementary to a steric structure of a microorganism to be detected, wherein said microorganism has entire electric charge or electric charge on a surface thereof that is excessively negatively charged, and wherein said polymer layer is made of polypyrrole, polvaniline, polythiophene, or derivatives thereof, said polymer layer being formed by a manufacturing method including: a polymerization step of polymerizing a monomer in presence of the microorganism to be detected, to form said polymer layer having captured said microorganism on said detection electrode; a destruction step of partially destroying the microorganism captured in said polymer layer; and a peroxidation step of peroxidizing said polymer layer to release said microorganism from said polymer layer, thereby imparting to said polymer layer a three-dimensional structure complementary to a steric structure of said microorganism, and (B) a counter electrode, wherein said sensor is adapted to supply alternating-current voltage between said detection electrode and said counter electrode in a state where said detection unit and said counter electrode are in contact with a sample solution, to direct said microorganism in said sample solution toward said detection unit by dielectrophoresis.