Patent ID: 6514941
Filing Date: 2003-02-04
Classification: A61K,C07K,C12P

Abstract:
A method of preparing a composition comprising C6, C7 and C12 peptides, said method comprising:i) hydrolyzing solubilized casein with a protease that has a substrate specificity such that said protease does not cleave said C6, C7 and C12 peptides, and ii) holding the hydrolysate resulting from step (i) under conditions such that the average molecular weight of said hydrolysate resulting from step (i) increases by at least 10%, iii) adjusting the pH of the hydrosylate resulting from step (ii) to between 5.5 and 10, and iv) size fractionating the hydrolysate resulting from step (iii) under conditions such that at least one fraction is obtained that comprises at least 2.5% by weight of said C6 peptide, at least one fraction is obtained that comprises at least 2.5% by weight of said C7 peptide and at least one fraction is obtained that comprises at least 2.5% by weight of said C12 peptide.