Patent ID: 9005927
Filing Date: 2015-04-14
Classification: C12N,C40B,G01N

Abstract:
1. A method of displaying a library comprising a diverse population of biologically active multi-chain polypeptides, each multi-chain polypeptide comprising at least two polypeptide chains, on the surface of diploid yeast cells, the method comprising: providing yeast cells, each yeast cell comprising: (i) a first polynucleotide encoding a polypeptide comprising a first chain of a biologically active multi-chain polypeptide linked to a cell surface anchor, and (ii) a second polynucleotide encoding a polypeptide comprising a second chain of the multi-chain polypeptide that is not linked to a cell surface anchor, wherein the yeast cells are capable of expression and secretion of the chains of the multi-chain polypeptides; and culturing the yeast cells under conditions sufficient to permit expression and association of the chains of the multi-chain polypeptides, wherein, for each yeast cell, the first chain of the multi-chain polypeptide is attached to the surface of the yeast cell by the cell surface anchor and the chains of the multi-chain polypeptide associate such that the biological activity of the multi-chain polypeptide is exhibited at the surface of the yeast cell.