Patent ID: 6358716
Filing Date: 2002-03-19
Classification: C12P

Abstract:
A method for the production of glycerol from a recombinant microorganism comprising:(i) transforming a suitable host cell with an expression cassette comprising (a) a gene encoding a NADH-dependent glycerol-3-phosphate dehydrogenase enzyme or a NADPH-dependent glycerol-3-phosphate dehydrogenase enzyme; and (b) a gene encoding a polypeptide encoding a glycerol-3-phosphatase (EC 3.1.3.21) enzyme; (ii) culturing the transformed host cell of (i) in the presence of at least one carbon source selected from the group consisting of monosaccharides, oligosaccharides, polysaccharides, and single-carbon substrates, whereby glycerol is produced; and (iii) recovering the glycerol produced in (ii).