Patent ID: 6531647
Filing Date: 2003-03-11
Classification: C12N

Abstract:
A method for silencing a stably integrated transgene target nucleotide sequence present in a first part of a Nicotiana plant, which method comprises transiently introducing a nucleic acid construct into the cytoplasm of a cell in a second part of the plant, which cell comprises the stably integrated transgene target nucleotide sequence and which is remote from said first part of the plant, wherein said construct:(i) encodes a sequence which shares sequence identity with the stably integrated transgene target nucleotide sequence or the complement thereof, and (ii) does not encode proteins which are capable of blocking systemic movement of a gene silencing signal, such that a gene silencing signal not comprising the construct initiated in the second part of the plant and propagated to the first part of the plant such as to cause the silencing of said target nucleotide sequence in the first part of the Nicotiana plant.