Patent ID: 8455401
Filing Date: 2013-06-04
Classification: B01J,C12Q

Abstract:
1. A method of amplifying nucleic acid template, comprising: a) providing: b) contacting said first surface with said first solution under conditions such that at least a portion of said first PCR primers attach to at least a portion of said first surface so as to create a modified surface comprising attached molecules; c) contacting said modified surface with said second solution; d) positioning said second surface on top of said modified surface, so as to create a plurality of first regions defined by said indentations and second regions defined by contact of said surfaces, said first regions comprising unmasked attached molecules, and said second regions comprising masked attached molecules, t hereby making a device; e) thermocycling said device under conditions such that at least a portion of said template is amplified by said unmasked attached molecules.