Patent ID: 6534646
Filing Date: 2003-03-18
Classification: C07H,Y10T

Abstract:
A reagent for use in oligonucleotide wherein the reagent is selected from the group:R1â€”Oâ€”CH2â€”CH(Oâ€”R2)â€”CH2â€”NHâ€”COâ€”(CH2)m-COâ€”NHâ€”(CH2)n-NHâ€”R3â€ƒâ€ƒ(Structure 1) R1â€”Oâ€”(CH2)n-NHâ€”COâ€”CH2)m-COâ€”NHâ€”CH2â€”CH(Oâ€”R2)â€”CH2â€”NHâ€”R3â€ƒâ€ƒ(Structure 2a) R1â€”Oâ€”(CH2)n-COâ€”NHâ€”CH2â€”CH(Oâ€”R2)â€”CH2â€”NHâ€”R3â€ƒâ€ƒ(Structure 2b) andR1â€”Oâ€”(CH2)3â€”COâ€”NHâ€”CH2â€”CH(OR2)â€”CH2â€”NHâ€”COâ€”(CH2)m-COâ€”NHâ€”(CH2)n-NHâ€”R3â€ƒâ€ƒ(Structure 3) wherein:R1 is 4,4â€²-dimethoxytrityl, 4-monomethoxytrityl or any other hydroxyl protecting group stable to oligonucleotide synthesis conditions; R2 is either selected from the group consisting of: and salts thereof, wherein R5 and R6 are independently selected from the group consisting of C3 to C10 branched alkyl, C1 to C12 unbranched alkyl, and cyclic hydrocarbons, and Y is any phosphate protecting group,or R2 includes a cleavable or noncleavable linkage to (and including) a support material; R3 is a label or reporter molecule; m is between 2 and 4, inclusive; and n is between 2 and 100, inclusive.