Patent ID: 9075013
Filing Date: 2015-07-07
Classification: G01N

Abstract:
1. A method according to forming a super-resolution image of an object that fluoresces at an activation wavelength and is inhibited from fluorescing at an inhibition wavelength, comprising: a) using light having the inhibition wavelength, sequentially forming n b) illuminating the object with light of the activation wavelength for each of the n c) capturing n d) for each of the sparse object images, de-convolving each of the emission-area images to obtain emission-area image intensities; e) combining the emission-area image intensities from the n further comprising performing a calibration that comprises at least one of: i) measuring and compensating for a distortion in the interference-pattern image; and ii) measuring and compensating for a non-uniformity of the activation-wavelength illumination.