Patent ID: 6110688
Filing Date: 2000-08-29
Classification: A61P,C07K,G01N,Y10S,Y10T

Abstract:
A process for purifying a protein and identifying the purified protein as Tamm-Horsfall glycoprotein or uromodulin which comprises the steps:freezing human urine;thawing the frozen urine to allow a precipitate to settle in a resulting solution,separating out the precipitate from the solution by centrifugation;recovering by filtration the centrifuged precipitate;dissolving the recovered precipitate in a phosphate-buffered saline solution;dialysis of the dissolved precipitate against phosphate buffer saline effecting a dialyzate;centrifuging the dialyzate to effect a supernatant;separating out the supernatant, followed by concentration of the supernatant to effect a concentrate;subjecting the concentrate to high performance liquid chromatography to isolate the protein-containing fraction;subjecting the protein-containing fraction to gel permeation to effect purified protein; andidentifying said purified protein by contacting the purified protein with anti-Tamm-Horsfall glycoprotein antibody, whereupon, interaction with said antibody identifies the purified protein as Tamm-Horsfall glycoprotein and no interaction with said antibody identifies said purified protein as uromodulin.