Patent ID: 8993280
Filing Date: 2015-03-31
Classification: C12N,C12P

Abstract:
1. A method for producing reticuline from dopamine, which comprises the steps of: providing a recombinant host cell expressing monoamine oxidase, norcoclaurine-6-O-methyltransferase, coclaurine-N-methyltransferase and 3′-hydroxy-N-methylcoclaurine-4′-O-methyltransferase, wherein the recombinant host cell is obtained by introducing genes encoding monoamine oxidase, norcoclaurine-6-O-methyltransferase, coclaurine-N-methyltransferase and 3′-hydroxy-N-methylcoclaurine-4′-O-methyltransferase into an isoquinoline alkaloid non-producing cell, and culturing the recombinant host cell in the presence of dopamine, wherein the recombinant host cell is an wherein the genes encoding monoamine oxidase consist of: (a) a nucleotide sequence consisting of SEQ ID NO: 1; or (b) a nucleotide sequence having 80% or more homology with the nucleotide sequence of SEQ ID NO: 1, and encoding a protein having monoamine oxidase activity, wherein the genes encoding norcoclaurine-6-O-methyltransferase consist of: (a) a nucleotide sequence consisting of SEQ ID NO: 3; or (b) a nucleotide sequence having 80% or more homology with the nucleotide sequence of SEQ ID NO: 3, and encoding a protein having norcoclaurine-6-O-methyltransferase activity, wherein the genes encoding coclaurine-N-methyltransferase consist of: (a) a nucleotide sequence consisting of SEQ ID NO: 4; or (b) a nucleotide sequence having 80% or more homology with the nucleotide sequence of SEQ ID NO: 4, and encoding a protein having coclaurine-N-methyltransferase activity, wherein the genes encoding 3′-hydroxy-N-methylcoclaurine-4′-O-methyltransferase consist of: (a) a nucleotide sequence consisting of SEQ ID NO: 5; or (b) a nucleotide sequence having 80% or more homology with the nucleotide sequence of SEQ ID NO: 5, and encoding a protein having 3′-hydroxy-N-methylcoclaurine-4′-O-methyltransferase activity.