Patent ID: 7348404
Filing Date: 2008-03-25
Classification: C07K,Y02P

Abstract:
1. A method of synthesizing a modified peptide having the following structure: wherein, the pre-sequence peptide is a homooligomeric peptide sequence consisting of from about 3 to about 9 amino acid residues having a propensity factor P the target peptide is a peptide having the structure: AA is an L or D amino acid residue, X is hydrogen or an amino protective group, Y is OH or NH n is an integer between 2 and 60; said method comprising: (a) coupling a pre-sequence peptide to a support, wherein said pre-sequence peptide comprises amino acid residues having side chain functionalities which are, if necessary, protected during the synthesis, (b) coupling one or more N-α-protected amino acids to the N-terminus of the pre-sequence peptide to form said target peptide, wherein each coupling is performed in stepwise fashion and under conditions in which each of the amino acids of the target peptide is coupled and subsequently N-α-de-protected; wherein the pre-sequence peptide reduces or eliminates propensity of the peptide of interest to adopt a β-sheet structure during the coupling and increases the coupling efficiency during the synthesis of the target peptide in comparison to the synthesis of the target peptide prepared under the same conditions without said pre-sequence peptide; and (c) cleaving said modified peptide from the support.