Patent ID: 8597615
Filing Date: 2013-12-03
Classification: C07K,G01N

Abstract:
1. A method for screening for agents that inhibit the induction of a human anti-human antibody (HAHA) response, said method comprising: administering a foreign human antibody to a transgenic mouse, said transgenic mouse comprising a collection of human immunoglobulin genes in its genome that following B cell immunoglobulin gene rearrangements in said transgenic mouse produce at least one human or humanized antibody from said human immunoglobulin genes, wherein said foreign human antibody is encoded by at least one human immunoglobulin gene that is not present in the genome of the transgenic mouse, wherein the foreign human antibody is capable of inducing a HAHA response, wherein the HAHA response comprises the human or humanized antibody produced by the transgenic mouse binding to the foreign human antibody, and wherein the human or humanized antibody produced by the transgenic mouse binds to the foreign human antibody in the absence of a candidate HAHA inhibitor, administering the candidate HAHA inhibitor to the transgenic mouse; and observing if a resulting HAHA response is inhibited after an amount of time sufficient to allow for the HAHA response.