Patent ID: 6110457
Filing Date: 2000-08-29
Classification: A61K,A61P,C07K,C12N

Abstract:
An enveloped, negative-sense, single-stranded RNA hybrid virus, the hybrid virus having a genome comprising, operatively linked for expression, (i) a nucleic acid sequence which encodes at least one surface antigen of an enveloped target virus of the family Paramyxoviridae, the surface antigen being different from surface antigens of cp45 and (ii) a nucleic acid sequence which encodes a variant HPIV-3 large protein, L, the variant L protein having at least one variation in amino acid sequence relative to the L protein of wild-type HPIV-3 (JS), the variation being a substitution selected from the group consisting of His for Tyr at residue 942 of SEQ ID NO. 1, Phe for Leu at residue 992 of SEQ ID NO. 1, and Ile for Thr at residue 1558 of SEQ ID NO. 1, the variant L protein having polymerase activity.