Patent ID: 7226780
Filing Date: 2007-06-05
Classification: C12N

Abstract:
1. An HIV-2 packaging vector comprising a 5′ splice donor site (SD) and an upstream and a downstream packaging signal sequence, wherein both the upstream and downstream packaging signal sequences are functionally deleted to reduce packaging of progeny viral RNA by more than 80%, but the SD is functionally intact, wherein functional deletion of the upstream and downstream packaging signal sequences comprises: deletion of nucleotides 306–458 upstream of the SD, and deletion of nucleotides 486–538 downstream of the SD; or deletion of nucleotides 306–370 upstream of the SD, and deletion of nucleotides 486–538 downstream of the SD; or deletion of nucleotides 371–458 upstream of the SD, and deletion of nucleotides 486–538 downstream of the SD.