Patent ID: 6194146
Filing Date: 2001-02-27
Classification: C07H,C12Q

Abstract:
A hybridization method for hybridizing labeled nucleic acid probes to nucleic acid sequences to form labeled hybridized complexes, comprising the steps of:a) denaturing nucleic acid sequences within an isolated population of biological matter;b) hybridizing labeled, nucleic acid probes having less than or equal to 75 bases with specific portions of the denatured nucleic acid sequences in the presence of a carbinol-based formamide-free hybridization buffer to form labeled hybridized complexes, the hybridization being carried out at a temperature between about 42.degree. C. and about 85.degree. C.; andc) washing the labeled hybridized complexes, the washing being carried out at a temperature between about 47.degree. C. and 55.degree. C.