Patent ID: 6600029
Filing Date: 2003-07-29
Classification: C12N,C12P

Abstract:
An expression cassette comprising a nucleic acid molecule encoding a polyhydroxyalkanoate monomer synthase operably linked to a promoter functional in a host cell, wherein the nucleic acid molecule comprises a first DNA segment encoding a first module and a second DNA segment encoding a second module, wherein the first and second modules each comprise a ketoacyl synthase, an acyl transferase, a ketoreductase, and an acyl carrier protein, wherein the first module comprises a loading module at the N-terminus, wherein the second module comprises a thioesterase at the C-terminus, wherein the DNA segments together encode a recombinant polyhydroxyalkanoate monomer synthase, wherein the loading module contains at least one domain which binds a substrate of the polyhydroxyalkanoate monomer synthase, and wherein at least one DNA segment is from Streptomyces venezuelae.