Patent ID: 8367349
Filing Date: 2013-02-05
Classification: A61P,C07K,C12Q,G01N

Abstract:
1. A method for assessing the effect of a test compound on LRRK2 in a cell-based system, the method comprising the steps of: a) assessing the effect of exposing the cell-based system comprising LRRK2 to the test compound on the phosphorylation state of Ser910 and/or Ser935 of the LRRK2; and/or b) assessing the effect of exposing the cell-based system comprising LRRK2 to the test compound on the binding of the LRRK2 to a 14-3-3 polypeptide and selecting a compound as being considered to have an inhibitory effect on LRRK2 in the cell-based system, wherein a test compound is so selected if the binding of the LRRK2 to the 14-3-3 polypeptide is reduced following exposure; and/or c) assessing the effect of exposing the cell-based system comprising LRRK2 to the test compound on the subcellular localization of LRRK2, wherein a test compound is selected as inhibitory if the accumulation of LRRK2 in cytoplasmic pools is increased following the exposure.