Patent ID: 6547827
Filing Date: 2003-04-15
Classification: A61L,A61P,Y10S

Abstract:
A method for forming a bioprosthesis including treatment of biological tissue which contains connective tissue protein to reduce the potential for post-implantation calcification and thrombogenicity, said method comprising the steps of:a. contacting the biological tissue with a mixture of a denaturant, a surfactant and a crosslinking agent; b. assembling the bioprosthesis by performing any required shaping of the biological tissue and attaching any non-biological components of the bioprosthesis to the biological tissue; and, c. after completion of steps a and b, as a final step of said method, immersing the bioprosthesis in a liquid terminal sterilization solution and heating said terminal sterilization to a temperature between about 35-50Â° C.