Patent ID: 6046013
Filing Date: 2000-04-04
Classification: G01N,Y10S

Abstract:
A process for detecting and identifying human antibody types which are specific for platelet glycoproteins, comprising:a) attaching a plurality of glycoprotein types to a solid support, each type unique from each other and separated from each other;b) testing each glycoprotein type for background specific to that glycoprotein against serum from one or from a pool of multiple non-immunized donors and measuring a numerical value for each glycoprotein type;c) attaching a pool of the different glycoprotein types to the solid support;d) testing the glycoprotein pool for background against serum from one or from a pool of two or more non-immunized donors and measuring a negative control numerical value for the glycoprotein pool;e) dividing the glycoprotein type value obtained from step b) by the negative control value from step d) to create a background adjustment factor for each glycoprotein type;f) adding a sample possibly containing antibodies to each glycoprotein type attached to the solid support;g) calculating a cutoff value for each glycoprotein type equal to the negative control numerical value from step (d) multiplied by a numerical value background adjustment factor for each glyconrotein from step (e); and,h) measuring a numerical value for each glycoprotein type mixed with a sample, wherein a measured numerical value greater than the cutoff value for any glycoprotein type indicates that antibodies specific for that glycoprotein type are present.