Patent ID: 8614059
Filing Date: 2013-12-24
Classification: C07K,Y10T

Abstract:
1. A method for simultaneously co-purifying and concentrating nucleic acid and protein targets, the method comprising: obtaining a sample comprising nucleic acids and protein; applying said sample to a hand-held apparatus comprising a portable device wherein the portable device comprises a gel-based isotachophoresis separation unit and a detection unit for the detection of an analyte selected from the group consisting of nucleic acid and protein, wherein the detection unit comprises disposable cartridges, subjecting said sample to a gel-based isotachophoresis on a cartridge of the portable device with the proviso that the isotachophoresis is not by the method of capillary electrophoresis, wherein the gel-based isotachophoresis comprises buffer formulations comprising Bis-tris MES solution in the gel, a bicine/NaOH cathode buffer and a Bis-tris/acetic acid solution as an anode buffer; and simultaneously co-purifying and concentrating nucleic acid and protein targets into a single volume in buffer by application of a voltage wherein an amount of charge imparted to the nucleic acid and protein targets causes simultaneous concentration of the nucleic acids and protein targets in a single volume; and obtaining the concentrated nucleic acid and protein targets from the hand-held apparatus; and detecting the presence of the nucleic acid and protein targets.