Patent ID: 6583131
Filing Date: 2003-06-24
Classification: C07J

Abstract:
A method for transfecting nucleic acids into cells, the method comprising: contacting said cells with a nucleic acid:lipid complex, wherein the complex comprises a nucleic acid and a compound having the structure:Xâ€”R2â€”R1â€”O-Cholesteryl, wherein R1 is a linker bond R2 is a direct bond, a branched or linear alkylene or alkenylene chain of 1 to 10 carbons in length, a C3-C7 cycloalkylene, or a phenylene; X is a saturated 4-7-membered nitrogen-containing cyclic structure; and X is linked to the R2 spacer via a carbon atom of the nitrogen-containing cyclic structure or via a nitrogen atom of the cyclic structure.