Patent ID: 6730832
Filing Date: 2004-05-04
Classification: A01H,C12N

Abstract:
A method for producing a modified tobacco plant line comprising an altered phenotype having an increase in at least one aspartate-derived branched amino acid selected from the group consisting of lysine, isoleucine, methionine, and threonine by selection for a mutation which results in the altered phenotype in the M1 generation, comprising the steps of:incubating seeds for the tobacco plant in a solution comprising a mutagen; washing the seeds free of the mutagen; germinating the seeds and growing M1 seedlings; adding a selection agent comprising lysine or threonine to the seedlings, wherein the selection agent selects for a chimeric plant, wherein the chimeric M1 plant at least partially comprises the altered phenotype of an increase in at least one aspartate-derived branched chain amino acid selected from the group consisting of lysine, isoleucine, methionine, and threonine; growing the M1 chimeric plant to generate M2 seeds, wherein the M2 seeds comprise non-mutagenized M2 seeds and mutagenized M2 seeds comprising the altered phenotype; and germinating the M2 seeds in a medium containing at least one selection agent comprising lysine or threonine to select for M2 plants comprising the altered phenotype of an increase in at least one aspartate-derived branched chain amino acid selected from the group consisting of lysine, isoleucine, methionine, and threonine.