Patent ID: 8163537
Filing Date: 2012-04-24
Classification: B01L,C12M

Abstract:
1. A nested permeable support device, comprising: a first sheet having an opening used to form a first well, where the first sheet has a top on which there is attached a gas permeable film and a bottom on which there is attached a first permeable support, where the gas permeable film has at least one opening formed therein to provide access only to the first well, and where the first permeable support is configured to allow a chemical entity to pass through but restrict movement there through of cells; a second sheet having an opening used to form a second well, where the second sheet has a top on which there is attached the first permeable support and a bottom on which there is attached a second permeable support, where each of the gas permeable film, the first sheet and the first permeable support has at least one opening formed therein to provide access only to the second well; and where the second permeable support is configured to allow the chemical entity to pass through but restrict movement there through of cells; and a third sheet having an opening used to form a third well, where the third sheet has a top on which there is attached the second permeable support and a bottom on which there is attached a receiver plate, and where each of the gas permeable film, the first sheet, the first permeable support, the second sheet and the second permeable support has at least one opening formed therein to provide access only to the third well.