Patent ID: 6379515
Filing Date: 2002-04-30
Classification: G01N

Abstract:
Process for the selective separation of electrically charged target molecules in an analytical mixture by means of capillary affinity gel electrophoresis, using a capillary tube which is at least partly filed with a polymer gel, which is solid, or a solution of the polymer in a solvent, having a viscosity of at least 20 mPa's, whereby receptors for target molecules are covalently bound to the polymer, and an electric filed of at least 50 volts/cm is applied, and wherein the process comprises (a) charging the capillary tube with the analytical mixture whereby the target molecules are bound to the receptors, (b) in a first stage, eluting remaining components of said analytical mixture under a first set of eluting conditions; (c) in a second stage, changing the eluting conditions to release said target molecules and eluting said target molecules, and (d) detecting said target molecules characterized in that the polymer comprises a copolymer containing structural elements with covalently bound receptors in a quantity of 0.01 to 99.9% by weight.