Patent ID: 7635467
Filing Date: 2009-12-22
Classification: A61K,A61P,C12N

Abstract:
1. Developmentally potent mesenchymal stem cells prepared according to a method comprising contacting mesenchymal stem cells with an effective amount of substituted deoxynucleotide or deoxynucleoside for an effective period, further comprising the step of co-culturing the mesenchymal stem cells contacted with substituted deoxynucleotide or deoxynucleoside with neural-lineage cells or media conditioned with neural-lineage cells, wherein the substituted deoxynucleotide or deoxynucleoside-contacted, mesenchymal stem cells become developmentally potent mesenchymal stem cells capable of differentiating to cells of neural lineage, with or without further comprising the step of contacting the mesenchymal stem cells with heparin, a growth factor or substituted deoxynucleotide or deoxynucleoside in an uncoated flask or a flask that has been treated to repel the cells, and wherein the substituted deoxynucleotide or deoxynucleoside is bromodeoxyuridine, bromodeoxycytidine, bromodeoxyguanosine, methyldeoxythymidine, or chlorodeoxyuridine.