Patent ID: 8722403
Filing Date: 2014-05-13
Classification: A01N,A61B

Abstract:
1. A method of biological specimen preservation for preserving a biological specimen, comprising: a. providing a bubble reservoir having a bottom wall and an open top; b. providing a holding platform having a top, a bottom, and at least one chamber passing through said holding platform from said top to said bottom, with said at least one chamber including a top opening and a bottom opening; c. locating said holding platform in said bubble reservoir with said bottom of said holding platform being separated from said bottom wall, thereby defining a space lying between said bottom of said holding platform and said bottom wall; d. providing a gas diffuser in said bubble reservoir, said gas diffuser lying outside said space lying between said bottom of said holding platform and said bottom wall; e. covering said bottom opening of said at least one chamber with a screen; f. filling said bubble reservoir with a preservation liquid to a level above said bottom of said holding platform but below said top of said holding platform, thereby flooding said at least one chamber to a level above said screen; g. placing said biological specimen in said at least one chamber, said biological specimen having a density greater than said preservation liquid so that said biological specimen sinks to said screen covering said bottom of said at least one chamber; h. said level of said preservation liquid being sufficient to completely submerge said biological specimen; i. forcing a gas including oxygen through said gas diffuser, thereby producing a plurality of gas bubbles in said preservation liquid, with said plurality of gas bubbles having a maximum bubble diameter and a minimum bubble diameter; and j. wherein said screen has a mesh size small enough to prevent the passage of said minimum bubble diameter.