Patent ID: 8445271
Filing Date: 2013-05-21
Classification: G01N

Abstract:
1. A process for determining drug resistance of cells of interest, said process comprising the steps of: (A) providing: (B) contacting cells from a first portion of said clinical or biological sample with said at least one xanthene compound; (C) contacting cells from a second portion of said clinical or biological sample (i) with said at least one xanthene compound and said one or more inhibitors; (D) measuring fluorescent signals generated in step (B) and step (C); and (E) comparing the fluorescent signals measured in step (D) to determine elevated activities of said MDR1, MRP and BCRP, or a combination thereof, thereby determining the drug resistance of said cells of interest, wherein said at least one xanthene compound has the structure wherein T and U are independently hydrogen, alkyl having 1-12 carbons, alkoxy having 1-12 carbons, aryloxy, amino, halogen, cyano, carboxy, carboxyalkyl, acetoxymethylcarbonyl, carbonyl, sulfonyl, phosphonyl, boronic acid, aryl, and heteroaryl, and V and W are independently selected from OR