Patent ID: 6159751
Filing Date: 2000-12-12
Classification: A61K,C07K,C12Q,Y10S

Abstract:
A method for preparing a hybridoma cell line which produces a monoclonal antibody which specifically recognizes and binds to a tumor associated antigen which comprises:a) cotransfecting a CREF-Trans 6 cell line (ATCC Accession No. CRL 10584) with DNA isolated from a neoplastic, human cell and a plasmid which encodes a selectable or identifiable trait;b) selecting transfected cells which express the selectable or identifiable trait;c) recovering the cells selected in step (b);d) injecting the cells so recovered in step (c) into a suitable first murine host;e) maintaining the resulting first murine host for a period of time effective to induce the cells injected in step (d) to form a tumor in the first murine host;f) isolating the tumor formed in step (e);g) obtaining tumor cells from the isolated tumor in step (f);h) coating the tumor cells obtained in step (g) with an antiserum generated against the CREE Trans-6 cell line;i) injecting the antiserum-coated cells from step (h) into a plurality of suitable second murine hosts;j) screening the resulting second hosts from step (i) to identify hosts which produce serum reactive with the neoplastic, human cell;k) removing spleens from the second hosts so identified in step (j);l) preparing from the spleens so removed in step (k) hybridomas; andm) recovering therefrom a hybridoma cell line which produces a monoclonal antibody which specifically recognizes and binds to the tumor associated antigen.