Patent ID: 8410334
Filing Date: 2013-04-02
Classification: C12N,Y02A

Abstract:
1. A non-natural plant having resistance to an invertebrate pest that ingests RNA from said plant, wherein said non-natural plant comprises a transgenic plant cell having in its genome a recombinant DNA construct that comprises a promoter functional in a plant cell and that is transcribed in said transgenic plant cell to a recombinant miRNA precursor; and said recombinant miRNA precursor comprises a single strand of RNA that folds into a secondary structure of a miRNA precursor identified from an invertebrate genome and that comprises at least one stem-loop that is processed to a mature miRNA; and said mature miRNA suppresses expression of at least one target gene of said invertebrate pest or of a symbiont associated with said invertebrate pest, thereby conferring on said non-natural plant resistance to said invertebrate pest.