Patent ID: 6107061
Filing Date: 2000-08-22
Classification: C12Q,Y10S

Abstract:
A method of identifying a nucleotide base at a predetermined location on a polynucleotide molecule for analysis, said method comprising,forming a hybrid polynucleotide molecule,said hybrid comprising,the polynucleotide for analysis,an extendable polynucleotide having a 3' terminus nucleotide base, wherein the extendable polynucleotide is hybridized to the polynucleotide for analysis, and wherein the 3' terminus nucleotide base is hybridized to nucleotide base on the polynucleotide for analysis that is located 3' adjacent to the nucleotide base at the predetermined location,extending the extendable polynucleotide by one nucleotide base,wherein the extending takes place in a reaction solution comprising,a first labeled terminator comprising a first nucleotide base and a first detectable label,a second labeled terminator comprising a second nucleotide base and a second detectable label, anda first corresponding unlabeled terminator comprising the first nucleotide base.