Patent ID: 8685654
Filing Date: 2014-04-01
Classification: A61P,G01N

Abstract:
1. A method for producing a vaccine, comprising the steps of: (i) preparing a bulk antigen of interest; (ii) analyzing a sample of the bulk antigen by performing an immunodiffusion assay, comprising the steps of: (a) obtaining a starting composition comprising an influenza virus hemagglutinin (HA) antigen of interest, wherein the HA antigen is adsorbed to an adjuvant that comprises an aluminium salt; (b) treating the starting composition to desorb the HA antigen from the adjuvant; (c) centrifuging a desorbed composition of (b); and (d) allowing supernatant from the centrifuged desorbed composition, or a sample thereof, to diffuse into a gel that contains antibody specific to the HA antigen of interest; and, based on the results of step (ii), (iii) diluting the bulk antigen to a desired final concentration; optionally (iv) combining the diluted bulk antigen with one or more pharmaceutically acceptable ingredients; and (v) packaging the diluted bulk antigen for distribution.