Patent ID: 8664396
Filing Date: 2014-03-04
Classification: C07F,C07K,G01N

Abstract:
1. A method of staining a poly(amino acid), comprising contacting the poly(amino acid) with a metal complex comprising: (i) at least one metal ion; and (ii) a plurality of donor ligands each fully coordinated to the metal ion and each independently selected from the group consisting of a nitrogen donor ligand and a cyclometalated donor ligand, wherein at least one of the donor ligands is a cyclometalated donor ligand; wherein: each nitrogen donor ligand comprises a heteroaryl ring system having from 10 to 40 ring atoms, wherein from 2 to 8 ring atoms are each independently selected from the group consisting of N, O and S, wherein at least two ring atoms are N, and wherein each nitrogen donor ligand is substituted with from 0 to 4 R each cyclometalated donor ligand is a member selected from the group consisting of: each R each R wherein the poly(amino acid) is present in a sample mixture on or in an SDS-PAGE gel, and wherein said metal complex is cationic in overall electronic charge, to achieve non-covalent association of said metal complex with the poly(amino acid) and to thereby stain the poly(amino acid).