Patent ID: 7651864
Filing Date: 2010-01-26
Classification: C12Q,G01N

Abstract:
1. A method of manufacturing an optical glucose sensor chip having a glucose sensing membrane containing an oxidizing enzyme or a reducing enzyme of glucose, a reagent for generating a substance for coloring a coloring agent, a coloring agent, and a membrane-forming polymer compound, comprising: preparing a first solution by dissolving the oxidizing enzyme or the reducing enzyme of glucose and a reagent for generating a substance for coloring a coloring agent in a buffer solution and then preserving the first solution; preparing a second solution by mixing an alcohol solution of the coloring agent and a solution of the membrane-forming polymer compound and then preserving the second solution; forming a pair of gratings on a glass substrate for receiving and sending light in the substrate; preparing a coating solution for the glucose sensing membrane by mixing the first solution and the second solution before the coating solution is applied to a substrate region among said gratings; and forming the glucose sensing membrane by applying the coating solution to the substrate region among the gratings and drying the solution; wherein the substrate region among the gratings is subjected to dry washing with excimer ultraviolet rays and wet treatment with an acid solution, before the coating solution is applied to the substrate region among the gratings.