Patent ID: 7601491
Filing Date: 2009-10-13
Classification: C12Q

Abstract:
1. A method of treating a biological sample to solubilize and extract nucleic acids therefrom comprising treating the sample with at least one protein denaturant and at least one aprotic solvent, wherein the at least one aprotic solvent is selected from the group consisting of formamide, dimethylformamide, dimethyl sulfoxide, dimethylacetamide, acetronitrile, benzene, toluene, acetone, cyclohexane, n-heptane, sulfur dioxide and hexamethylphosphoramide, wherein the at least one protein denaturant is a proteolytic enzyme selected from the group consisting of proteinase K, pronase, pepsin, trypsin, chymotrypsin, carboxypeptidase and elastase, and wherein the method is carried out at a temperature at or above 4° C.