Patent ID: 6114127
Filing Date: 2000-09-05
Classification: C07K,G01N

Abstract:
A method for identifying agonist or antagonist of a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:2, said method comprising the steps of:(a) contacting a cell expressing on the surface thereof the polypeptide, said polypeptide being associated with a second component capable of providing a detectable signal in response to the binding of a labeled or unlabeled ligand selected from the group consisting of: dexovadenosine triphoshate (""dATP""), deoxyadenosine monophosphate (""dAMP""). and uridine triphosphate (""UTP"") to said polypeptide, with a compound to be screened under conditions to permit binding to the polypeptide; and(b) determining whether the compound binds to and activates or inhibits the polypeptide by measuring the level of a signal generated from the interaction of the ligand with the polypeptide.