Patent ID: 9116155
Filing Date: 2015-08-25
Classification: G01N

Abstract:
1. A method for early diagnosis of acute coronary syndrome (ACS) in a human subject who has one or more symptoms of ACS, the method comprising: a) providing a sample comprising blood from a human subject suspected of having ACS obtained within 12 hours of arrival at a medical center; b) performing an immunoassay to determine a level of Troponin I (TnI) in a sample obtained from the human subject within 12 hours of arrival at a medical center; c) performing additional diagnostic analysis on the sample if the Troponin I (TnI) level is less than 0.04 ng/ml, wherein the additional diagnostic analysis comprises: contacting the sample obtained from the human subject with phosphorylcholine (PC) under conditions sufficient for binding of C-reactive protein (CRP) to the PC to occur in the sample; isolating the PC-bound CRP in the sample; fractionating the PC-bound CRP based on size; determining a level of one or more of the fractions of CRP in the sample (fracCRP) representing the level of CRP molecules that are not in the native pentameric ring form; calculating, using a suitably programmed computer, an interpretive score by multiplying the level of fracCRP by the level of TnI; comparing the interpretive score to a reference score, and diagnosing a subject who has a higher interpretive score than the reference score with ACS.