Patent ID: 8362216
Filing Date: 2013-01-29
Classification: A01K,A61K,A61P,C07K,C12N,G01N

Abstract:
1. A method for detecting an N-terminal or C-terminal fragment of a vertebrate hedgehog polypeptide, comprising: a) contacting a sample suspected of containing a hedgehog polypeptide or a fragment thereof with an antibody that specifically binds to the N-terminal or C-terminal fragment of the vertebrate hedgehog polypeptide or fragment thereof; and b) detecting binding of the antibody, wherein the N-terminal fragment or fragment thereof, has a Glycine reside at its carboxy terminus, resulting from specific cleavage of a G↓CF cleavage site specifically recognized by a proteolytic activity of the carboxy terminal fragment of a native hedgehog polypeptide, and wherein the C-terminal fragment or fragment thereof, has Cys Phe residues at its amino terminus, resulting from specific cleavage of a G↓CF cleavage site contained within the hedgehog polypeptide.