Patent ID: 7258986
Filing Date: 2007-08-21
Classification: A61K,C07K,C12N

Abstract:
1. A method for screening for intrabodies with CDRs interacting with a specific antigen comprising: (a) identification of intrabody framework which is soluble and stable in a reducing environment by a method which comprises the following steps: (b) preparation of a randomized intrabody CDR library by exchanging at least one nucleic acid sequence encoding a CDR in an intrabody with a framework which has been identified by the method of step a); (c) transformation of host cells with a randomized intrabody CDR library of step (b) and with a nucleic acid sequence encoding a known antigen, whereby the antigen and/or the intrabody of the CDR library are linked to a marker system or part of a marker system such that the cells cultured under selective conditions only survive in the presence of antigen/intrabody-interaction which activates the marker system; (d) cultivation of the cells under selective conditions allowing cell survival only in the presence of antigen/intrabody-interaction; and (e) harvesting the intrabodies interacting with the known antigen from surviving cells.