Patent ID: 6245887
Filing Date: 2001-06-12
Classification: C12N,C12Y

Abstract:
An isolated C-terminal .alpha.-amidating enzyme, having the following amino acid sequence (IV):Ser Leu Ser Asn Asp Cys Leu Gly Thr Thr Arg Pro Val Met Ser Pro Gly Ser Ser Asp Tyr Thr Leu Asp Ile Arg Met Pro Gly Val Thr Pro Thr Glu Ser Asp Thr Tyr Leu Cys Lys Ser Tyr Arg Leu Pro Val Asp Asp Glu Ala Tyr Val Val Asp Tyr Arg Pro His Ala Asn Met Asp Thr Ala His His Met Leu Leu Phe Gly Cys Asn Val Pro Ser Ser Thr Asp Asp Tyr Trp Asp Cys Ser Ala Gly Thr Cys Asn Asp Lys Ser Ser Ile Met Tyr Ala Trp Ala Lys Asn Ala Pro Pro Thr Lys Leu Pro Glu Gly Val Gly Phe Gln Val Gly Gly Lys Ser Gly Ser Arg Tyr Phe Val Leu Gln Val His Tyr Gly Asp Val Lys Ala Phe Gln Asp Lys His Lys Asp Cys Thr Gly Val Thr Val Arg Ile Thr Pro Glu Lys Gln Pro Leu Ile Ala Gly Ile Tyr Leu Ser Met Ser Leu Asn Thr Val Val Pro Pro Gly Gln Glu Val Val Asn Ser Asp Ile Ala Cys Leu Tyr Asn Arg Pro Thr Ile His Pro Phe Ala Tyr Arg Val His Thr His Gln Leu Gly Gln Val Val Ser Gly Phe Arg Val Arg His Gly Lys Trp Thr Leu Ile Gly Arg Gln Ser Pro Gln Leu Pro Gln Ala Phe Tyr Pro Val Glu His Pro Leu Glu Ile Ser Pro Gly Asp Ile Ile Ala Thr Arg Cys Leu Phe Thr Gly Lys Gly Arg Met Ser Ala Thr Tyr Ile Gly Gly Thr Ala Lys Asp Glu Met Cys Asn Leu Tyr Ile Met Tyr Tyr Met Asp Ala Ala His Ala Thr Ser Tyr Met Thr Cys Val Gln Thr Gly Asn Pro Lys Leu Phe Glu Asn Ile Pro Glu Ile Ala Asn Val Pro Ile Pro Val Ser Pro Asp Met Met Met Met Met Met Met Gly His Gly His-Cwherein C represents the following amino acid sequence (V):Gly Asp Pro Leu Glu Ser Thr Cys Arg His Ala, orthe following amino acid sequence (VI):His His Thr Glu Ala Glu -X- Glu -Y- Asn Thr -Z- Leu Gln Gln Pro Lys-Dwherein X represents Ala or Pro; Y represents Thr or Lys; Z represents Ala or Gly; and D is absent or represents the following amino acid sequence (VII), (VIII), (IX) or (X):(VII):\\Arg Glu Glu Glu Glu Val Leu Asn Gln Asp Val His Leu Glu Glu Asp Thr Asp Trp Pro Gly Val Asn Leu Lys Val Gly Gln Val Ser Gly Leu Ala Leu Asp Pro Lys Asn Asn Leu Val Ile Phe His Arg Gly Asp His Val Trp Asp Glu Asn Ser Phe Asp Arg Asn Phe Val Tyr Gln Gln Arg Gly Ile Gly Pro Ile Gln Glu Ser Thr Ile Leu Val Val Asp Pro Asn Thr Ser Lys Val Leu Lys Ser Thr Gly Gln Asn Leu Phe Phe Leu Pro His Gly Leu Thr Ile Asp Arg Asp Gly Asn Tyr Trp Val Thr Asp Val Ala Leu His Gln Val Phe Lys Val Gly Ala Glu Lys Glu Thr Pro Leu Leu Val Leu Gly Arg Ala Phe Gln Pro Gly Ser Asp Arg Lys His Phe Cys Gln Pro Thr Asp Val Ala Val Asp Pro Ile Thr Gly Asn Phe Phe Val Ala Asp Gly Tyr Cys Asn Ser Arg Ile Met Gln Phe Ser Pro Asn Gly Met Phe Ile Met Gln Trp Gly Glu Glu Thr Ser Ser Asn Leu Pro Arg Pro Gly Gln Phe Arg Ile Pro His Ser Leu Thr Met Ile Ser Asp Gln Gly Gln Leu Cys Val Ala Asp Arg Glu Asn Gly Arg Ile Gln Cys Phe His Ala Lys Thr Gly Glu Phe Val Lys Gln Ile Lys His Gln Glu Phe Gly Arg Glu Val Phe Ala Val Ser Tyr Ala Pro Gly Gly Val Leu Tyr Ala Val Asn Gly Lys Pro Tyr Tyr Gly Asp Ser Thr Pro Val Gln Gly Phe Met Leu Asn Phe Ser Asn Gly Asp Ile Leu Asp Thr Phe Ile Pro Ala Arg Lys Asn Phe Glu Met Pro His Asp Ile Ala Ala Gly Asp Asp Gly Thr Val Tyr Val Gly Asp Ala His Ala Asn Ala Val Trp Lys Phe Ser Pro Ser Lys Ala Glu His Arg Ser Val Lys Lys Ala Gly Ile Glu Val Glu Glu Ile Thr Glu Thr Glu Ile Phe Glu Thr His Met Arg Ser Arg Pro Lys Thr Asn Glu Ser Val Gly Gln Gln Thr Gln Glu Lys Pro Ser Val Val Gln Glu Ser Ser Ala Gly Val Ser Phe Val Leu Ile Ile Thr Leu Leu Ile Ile Pro Val Val Val Leu Ile Ala Ile Ala Ile Phe Ile Arg Trp Arg Lys Val Arg Met Tyr Gly Gly Asp Ile Gly His Lys Ser Glu Ser Ser Ser Gly Gly Ile Leu Gly Lys Leu Arg Gly Lys Gly Ser Gly Gly Leu Asn Leu Gly Thr Phe Phe Ala Thr His Lys Gly Tyr Ser Arg Lys Gly Phe Asp Arg Leu Ser Thr Glu Gly Ser Asp Gln Glu Lys Asp Asp Asp Asp Asp Gly Ser Asp Ser Glu Glu Glu Tyr Ser Ala Pro Pro Ile Pro Pro Val Ser Ser Ser; (VIII): Arg Glu Glu Glu Glu Val Leu Asn Gln Asp Val His Leu Glu Glu Asp Thr Asp Trp Pro Gly Val Asn Leu Lys Val Gly Gln Val Ser Gly Leu Ala Leu Asp Pro Lys Asn Asn Leu Val Ile Phe His Arg Gly Asp His Val Trp Asp Glu Asn Ser Phe Asp Arg Asn Phe Val Tyr Gln Gln Arg Gly Ile Gly Pro Ile Gln Glu Ser Thr Ile Leu Val Val Asp Pro Asn Thr Ser Lys Val Leu Lys Ser Thr Gly Gln Asn Leu Phe Phe Leu Pro His Gly Leu Thr Ile Asp Arg Asp Gly Asn Tyr Trp Val Thr Asp Val Ala Leu His Gln Val Phe Lys Val Gly Ala Glu Lys Glu Thr Pro Leu Leu Val Leu Gly Arg Ala Phe Gln Pro Gly Ser Asp Arg Lys His Phe Cys Gln Pro Thr Asp Val Ala Val Asp Pro Ile Thr Gly Asn Phe Phe Val Ala Asp Gly Tyr Cys Asn Ser Arg Ile Met Gln Phe Ser Pro Asn Gly Met Phe Ile Met Gln Trp Gly Glu Glu Thr Ser Ser Asn Leu Pro Arg Pro Gly Gln Phe Arg Ile Pro His Ser Leu Thr Met Ile Ser Asp Gln Gly Gln Leu Cys Val Ala Asp Arg Glu Asn Gly Arg Ile Gln Cys Phe His Ala Lys Thr Gly Glu Phe Val Lys Gln Ile Lys His Gln Glu Phe Gly Arg Glu Val Phe Ala Val Ser Tyr Ala Pro Gly Gly Val Leu Tyr Ala Val Asn Gly Lys Pro Tyr Tyr Gly Asp Ser Thr Pro Val Gln Gly Phe Met Leu Asn Phe Ser Asn Gly Asp Ile Leu Asp Thr Phe Ile Pro Ala Arg Lys Asn Phe Glu Met Pro His Asp Ile Ala Ala Gly Asp Asp Gly Thr Val Tyr Val Gly Asp Ala His Ala Asn Ala Val Trp Lys Phe Ser Pro Ser Lys Ala Glu His Arg Ser Val Lys Lys Ala Gly Ile Glu Val Glu Glu Ile Thr Glu Thr Glu Ile Leu; (IX):Arg Glu Glu Glu Glu Val Leu Asn Gln Asp Val His Leu Glu Glu Asp Thr Asp Trp Pro Gly Val Asn Leu Lys Val Gly Gln Val Ser Gly Leu Ala Leu Asp Pro Lys Asn Asn Leu Val Ile Phe His Arg Gly Asp His Val Trp Asp Glu Asn Ser Phe Asp Arg Asn Phe Val Tyr Gln Gln Arg Gly Ile Gly Pro Ile Gln Glu Ser Thr Ile Leu Val Val Asp Pro Asn Thr Ser Lys Val Leu Lys Ser Thr Gly Gln Asn Leu Phe Phe Leu Pro His Gly Leu Thr Ile Asp Arg Asp Gly Asn Tyr Trp Val Thr Asp Val Ala Leu His Gln Val Phe Lys Val Gly Ala Glu Lys Glu Thr Pro Leu Leu Val Leu Gly Arg Ala Phe Gln Pro Gly Ser Asp Arg Lys His Phe Cys Gln Pro Thr Asp Val Ala Val Asp Pro Ile Thr Gly Asn Phe Phe Val Ala Asp Gly Tyr Cys Asn Ser Arg Ile Met Gln Phe Ser Pro Asn Gly Met Phe Ile Met Gln Trp Gly Glu Glu Thr Ser Ser Asn Leu Pro Arg Pro Gly Gln Phe Arg Ile Pro His Ser Leu Thr Met Met Gly Ile Leu; (X):Arg Glu Glu Glu Glu Val Leu Asn Gln Asp Val His Leu Glu Glu Asp Thr Asp Trp Pro Gly Val Asn Leu Lys Val Gly Gln Val Ser Gly Leu Ala Leu Asp Pro Lys Asn Asn Leu Val Ile Phe His Arg Gly Asp His Val Trp Asp Glu Asn Ser Phe Asp Arg Asn Phe Val Tyr Gln Gln Arg Gly Ile Gly Pro Ile Gln Glu Ser Thr Ile Leu Val Val Asp Pro Asn Thr Ser Lys Val Leu Lys Ser Thr Gly Gln Asn Leu Phe Phe Leu Pro His Gly Leu Thr Ile Asp Arg Asp Gly Asn Tyr Trp Val Thr Asp Val Ala Leu His Gln Val Phe Lys Val Gly Ala Glu Lys Glu Thr Pro Leu Leu Val Leu Gly Arg Ala Phe Gln Pro Gly Ser Asp Arg Lys His Phe Cys Gln Pro Thr Asp Val Ala Val Asp Leu Gln Ala Cys Leu Ile Asn.