Patent ID: 8728410
Filing Date: 2014-05-20
Classification: B01D,B03C,C07K,C12N,Y10T

Abstract:
1. A device for facilitating extraction of a fraction from a biological sample, the biological sample including a fraction, non-desired material and a solid phase substrate, the device comprising: an input zone for receiving the biological sample therein, the fraction binding to the solid phase substrate to form a fraction-bound solid phase substrate; a phase-gate zone communicating with the input zone and receiving an isolation buffer therein; an elution zone communicating with the phase-gate zone and receiving a reagent therein, the reagent extracting the fraction from the fraction-bound solid phase substrate; a wash zone having an input communicating with the elution zone and an output communicating with the input zone, the wash zone receiving a wash buffer therein for regenerating the solid phase substrate; and a force movable between a first position adjacent the input zone, a second position adjacent the phase-gate zone, a third position adjacent the elution zone, and a fourth position adjacent the wash zone; wherein the force sequentially moves: from the first position to the second position so as to urge the fraction-bound solid phase substrate from the input zone to the phase-gate zone; from the second position to the third position so as to urge the fraction-bound solid phase substrate from the phase-gate zone to the elution zone wherein the fraction is extracted from the fraction-bound solid phase substrate such that solid phase substrate remains; from the third position to the fourth position so as to urge the solid phase substrate from the elution zone to the wash zone wherein the solid phase substrate is regenerated; and from the fourth position to the first position so as to urge the solid phase substrate from the wash zone to the input zone wherein the solid phase substrate is free to bind with an additional fraction.