Patent ID: 6521446
Filing Date: 2003-02-18
Classification: G01N,Y10S

Abstract:
A method of detecting the presence of glucose in a test sample comprisingproviding a glucose biosensor comprising a glucose binding protein (GBP) and a reporter group that transduces a detectable signal, wherein said reporter group is attached to said GBP so that a signal transduced by said reporter group when said GBP is bound to glucose differs from a signal transduced by said reporter group when said GBP is not bound to glucose; contacting said biosensor with said test sample under conditions such that said biosensor can bind to glucose present in said test sample; and comparing the signal transduced by said reporter group when said biosensor is contacted with said test sample with the signal transduced by said reporter group when said biosensor is contacted with a glucose-free control sample, wherein a difference in the signal transduced by said reporter group when said biosensor is contacted with said test sample, as compared to when said biosensor is contacted with said control sample, indicates that test sample contains glucose.