Patent ID: 6653462
Filing Date: 2003-11-25
Classification: C07H,C12Q

Abstract:
A nucleoside/tide compound comprising the structure: wherein:NUC is a nucleoside/tide having a nucleobase B; L is a rigid linkage having the structure: wherein each of n, o and p are integers ranging from 0 to 3, and the sum of n, o and p is at least 2; and each of W, X, Y and Z is independently selected from CH and N, including substituted forms thereof; S is a spacer selected from C1-C8 lower alkyl, lower alkylene oxide, amide, carbamate, and sulfonamide; wherein lower alkylene oxide is selected from the structures: &Parenopenst;Oâ€”CH2â€”CH2&Parenclosest;n, â€”CH2â€”Oâ€”(CH2)nâ€”, and â€”Oâ€”(CH2)nâ€”where n is an integer from 1 to 8; and LB/LG is an amino group, a protected amino or a label, wherein label is selected from a rhodamine-type dye and a fluorescein-type dye; wherein NUC is attached to L through the nucleobase B such that when B is a purine, L is attached to the 8-position of the purine, when B is 7-deazapurine, L is attached to the 7-position of the 7-deazapurine, and when B is pyrimidine, L is attached to the 5-position of the pyrimidine.