Patent ID: 6379909
Filing Date: 2002-04-30
Classification: G01N,Y10S

Abstract:
A method of evaluating immunological status of a subject comprising the steps of:A) determining content of an antibody in a liquid sample from the subject using the following assay protocol; (Aa) mixing (i) the antibody of the sample, (ii) a reactant antibody directed against an Fc region of the sample antibody, the reactant antibody being bound to a solid carrier and (iii) a ligand selected from an antigen, an antibody or a hapten, the ligand being directed to an Fab region of the sample antibody, to form a mixture of a three-component solid phase complex and a liquid phase, (Ab) contacting the three-component complex with (iv) a label compound to form a mixture of a four-component complex and a liquid phase, (Ac) washing the four-component solid phase to remove non-complex bound compounds, (Ad) detecting and measuring the washed labeled four-component complex to obtain a measurement A; (B) determining content of said antibody in said sample using the following assay protocol: (Ba) mixing (i) the antibody of the sample, and (ii) a reactant antibody directed against the Fc region of the sample antibody, the reactant antibody being bound to a solid carrier, to form a mixture of a two-component solid phase complex and a liquid phase, (Bb) washing the two-component solid phase complex to remove noncomplex bound compounds, (Bc) contacting the washed two-component solid phase complex with a (iii) a ligand selected from an antigen, an antibody or a hapten, the ligand being bound to the Fab region of the sample antibody, and (iv) a label compound, to form a mixture of a four-component solid phase complex and a liquid phase, (Bd) washing the four-component solid phase complex to remove noncomplex bound compounds, (Be) detecting and measuring the washed labeled four-component complex to obtain a measurement B; and (E) interrelating measurements A and B to express an interference and using the interference as a parameter for evaluating the immunological status of the subject.