Patent ID: 6787356
Filing Date: 2004-09-07
Classification: C12N

Abstract:
A method of generating a cell culture comprising dopaminergic neuron cells, said method comprising the sequential steps of:a. providing precursor cells comprising human or rat fetal central nervous system cells; b. proliferating precursor cells, said step of proliferating comprising: i. incubating a suspension of said precursor cells in a proliferating medium which includes basic fibroblast growth factor (bFGF) to form proliferated precursor cells; and subsequently c. differentiating said proliferated precursor cells, said step of differentiating comprising: i. incubating said proliferated precursor cells in an incubation vessel which contains differentiation medium in a manner effective to form a reaggregation of differentiated dopaminergic neuron cells that is not adhered to any surface of the incubation vessel, wherein the differentiation medium includes ascorbic acid.