Patent ID: 7736891
Filing Date: 2010-06-15
Classification: G01N

Abstract:
1. A microfluidic assay system comprising: (a) an input channel having an upstream end and a downstream end; (b) an injection channel that intersects with the input channel between the upstream end and the downstream end of the input channel, wherein the injection channel has a receiving end and a terminus disposed at opposing sides of the intersection with the input channel; (c) a reagent channel and a dilution channel, each having an upstream end and a downstream end, in communication with the injection channel, wherein the dilution channel is in series with and between the reagent channel and the injection channel; (d) an assay channel having an upstream end, a downstream end, and a surface that receives fluid flowing from the downstream end of the input channel toward the downstream end of the assay channel; (e) a capture region disposed on the surface of the assay channel and to which a detector molecule is bound; and (f) a reference region disposed on the surface of the assay channel; (g) detection means for detecting an amount of binding between the capture region and an analyte and/or reagent and for detecting an amount of a tracer present in the reference region; and (h) analysis means in communication with the detection means and that correlates the amount of binding detected at the capture region to the amount of tracer detected in the reference region.