Patent ID: 6967107
Filing Date: 2005-11-22
Classification: C07D

Abstract:
1. A method of conjugating a first species selected from the group consisting of a protein, a polypeptide, an antibody, a nucleic acid, a small molecule, an aptamer, and a carbohydrate, to a second species selected from the group consisting of a detectable label, a solid phase, a protein, a polypeptide, an antibody, a nucleic acid, a small molecule, an aptamer, and a carbohydrate, the method comprising: contacting said first and second species with a crosslinker of the formula: wherein X is (alkylene oxide) n is between about 40 to about 450; and Y and Z are independently arylene or heteroarylene units having 5 or 6 ring atoms, optionally substituted with from 1 to 4 substituents independently selected from the group consisting of C under conditions selected to conjugate said first species to said second species, wherein one of the first or second species becomes covalently bound to said crosslinker through a moiety reactive with an N-hydroxysuccinimide functional group, and the other of the first or second species becomes covalently bound to said crosslinker through a moiety reactive with a maleimide functional group wherein the conjugating step comprises (i) covalent binding of the crosslinker to the first or second species through a moiety reactive with a first functional group that is an N-hydroxysuccinimide or a maleimide functional group, to form a covalently bound crosslinker intermediate; (ii) removal of unreacted crosslinker from the covalently bound crosslinker intermediate; and (iii) covalent binding of the crosslinker to the other of the first or second species through a moiety reactive with a second functional group that is the other of the N-hydroxysuccinimide or maleimide functional group.