Patent ID: 6770746
Filing Date: 2004-08-03
Classification: A61K,A61P,C07K

Abstract:
A method of making a substantially homogenous preparation of a monosuccinylated protein comprising the steps of: (a) reacting a protein with 3-7 fold molar excess of succinic anhydride to form a reaction mixture; (b) stirring said reaction mixture 2-16 hours at 4Â° C.; (c) elevating the pH of said reaction mixture to 8.5 using 5N NaOH; (d) stirring said reaction mixture another 1-2 hours at 4Â° C.; (e) dialyzing said reaction mixture against 20 mM Tris-HCl, pH 7.2; and (f) isolating said monosuccinylated protein from said reaction mixture.