Patent ID: 6280738
Filing Date: 2001-08-28
Classification: A61K,A61P,C07K,Y10S

Abstract:
A polysaccharide-protein conjugate comprising a mutant C.beta. protein conjugated to a streptococcal capsular polysaccharide, wherein the mutant C.beta. protein comprises an amino acid sequence A-X.sub.202 X.sub.203 X.sub.204 X.sub.205 X.sub.206 X.sub.207 X.sub.208 X.sub.209 X.sub.210 X.sub.211 X.sub.212 X.sub.213 -B, wherein A comprises in sequence amino acid residues 38-201 of SEQ ID NO: 2, B represents a sequence starting from amino acid 214 of SEQ ID NO: 2 and terminating at an amino acid between residues 1131 and 1164, inclusive, of SEQ ID NO: 2, and X.sub.202 through X.sub.213 are each selected independently from the group consisting of Ala, Val, Leu, Ile, Pro, Met, Phe, Trp, a bond, and a wild-type amino acid as found at a corresponding position of residues 202-213 of SEQ ID NO: 2, with the proviso that at least one of X.sub.202 through X.sub.213, inclusive, is other than the wild type amino acid found at the corresponding position of SEQ ID NO: 2, wherein said mutant C.beta. protein exhibits reduced or no IgA binding activity in comparison to native C.beta. protein which comprises the amino acid sequence of residue 38 through at least residue 1131 of SEQ ID NO: 2, and wherein antigenicity of the mutant C.beta. protein in comparison to the native protein is substantially retained.