Patent ID: 6413750
Filing Date: 2002-07-02
Classification: C12N,C12P

Abstract:
â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒSEQUENCEâ€ƒLISTING  <160>â€ƒNUMBERâ€ƒOFâ€ƒSEQâ€ƒIDâ€ƒNOS:â€ƒ27 <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ1<211>â€ƒLENGTH:â€ƒ31<212>â€ƒTYPE:â€ƒDNA<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒprimerâ€ƒBRS <400>â€ƒSEQUENCE:â€ƒ1 gcgcgaattcâ€ƒatgtctaaccâ€ƒgtttggatggâ€ƒtâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ31  <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ2<211>â€ƒLENGTH:â€ƒ52<212>â€ƒTYPE:â€ƒDNA<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒprimerâ€ƒBRAS <400>â€ƒSEQUENCE:â€ƒ2 gcgcaagcttâ€ƒctactattgaâ€ƒgcagtgtagcâ€ƒcaccgtcaacâ€ƒtacaaattcaâ€ƒg #aâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ52  <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ3<211>â€ƒLENGTH:â€ƒ36<212>â€ƒTYPE:â€ƒDNA<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒprimerâ€ƒR38LK46I <400>â€ƒSEQUENCE:â€ƒ3 accggcctgcâ€ƒacagcgatgtâ€ƒtggtgaaataâ€ƒgcagctâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ36  <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ4<211>â€ƒLENGTH:â€ƒ36<212>â€ƒTYPE:â€ƒDNA<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒprimerâ€ƒR38LK46Iâ€ƒrev <400>â€ƒSEQUENCE:â€ƒ4 agctgctattâ€ƒtcaccaacatâ€ƒcgctgtgcagâ€ƒgccggtâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ36  <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ5<211>â€ƒLENGTH:â€ƒ21<212>â€ƒTYPE:â€ƒDNA<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒprimerâ€ƒA9G <400>â€ƒSEQUENCE:â€ƒ5 ggtaaggtagâ€ƒgaatcattacâ€ƒaâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#21  <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ6<211>â€ƒLENGTH:â€ƒ21<212>â€ƒTYPE:â€ƒDNA<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒprimerâ€ƒA9Gâ€ƒrev <400>â€ƒSEQUENCE:â€ƒ6 tgtaatgattâ€ƒcctaccttacâ€ƒcâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#21  <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ7<211>â€ƒLENGTH:â€ƒ30<212>â€ƒTYPE:â€ƒDNA<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒprimerâ€ƒR38LK45M <400>â€ƒSEQUENCE:â€ƒ7 accggcctgcâ€ƒacagcgatgtâ€ƒtgaaatggcaâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ30  <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ8<211>â€ƒLENGTH:â€ƒ33<212>â€ƒTYPE:â€ƒDNA<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒprimerâ€ƒR38LK45Mâ€ƒrev <400>â€ƒSEQUENCE:â€ƒ8 tgccatttcaâ€ƒccaacatcgcâ€ƒtgtgcaggccâ€ƒggtâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ33  <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ9<211>â€ƒLENGTH:â€ƒ45<212>â€ƒTYPE:â€ƒDNA<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒprimerâ€ƒR38LK48M <400>â€ƒSEQUENCE:â€ƒ9 accggcctgcâ€ƒacagcgatgtâ€ƒtggtgaaaaaâ€ƒgcagctatgaâ€ƒgtgtcâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#45  <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ10<211>â€ƒLENGTH:â€ƒ45<212>â€ƒTYPE:â€ƒDNA<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒprimerâ€ƒR38LK48Mâ€ƒrev <400>â€ƒSEQUENCE:â€ƒ10 gacactcataâ€ƒgctgctttttâ€ƒcaccaacatcâ€ƒgctgtgcaggâ€ƒccggtâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#45  <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ11<211>â€ƒLENGTH:â€ƒ21<212>â€ƒTYPE:â€ƒDNA<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒprimerâ€ƒR38LH39L <400>â€ƒSEQUENCE:â€ƒ11 accggcctgcâ€ƒtcagcgatgtâ€ƒtâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#21  <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ12<211>â€ƒLENGTH:â€ƒ21<212>â€ƒTYPE:â€ƒDNA<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒprimerâ€ƒR38LH39Lâ€ƒrev <400>â€ƒSEQUENCE:â€ƒ12 aacatcgctgâ€ƒagcaggccggâ€ƒtâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#21  <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ13<211>â€ƒLENGTH:â€ƒ45<212>â€ƒTYPE:â€ƒDNA<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒG37DR38LK48M <400>â€ƒSEQUENCE:â€ƒ13 accgacctgcâ€ƒacagcgatgtâ€ƒtgttgaaaaaâ€ƒgcagctatgaâ€ƒgtgtcâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#45  <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ14<211>â€ƒLENGTH:â€ƒ45<212>â€ƒTYPE:â€ƒDNA<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒG37DR38LK48Mâ€ƒâ€ƒrev <400>â€ƒSEQUENCE:â€ƒ14 gacactcataâ€ƒgctgctttttâ€ƒcaccaacatcâ€ƒgctgtgcaggâ€ƒtcggtâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#45  <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ15<211>â€ƒLENGTH:â€ƒ50<212>â€ƒTYPE:â€ƒPRT<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒwild-type <400>â€ƒSEQUENCE:â€ƒ15 Serâ€ƒAsnâ€ƒArgâ€ƒLeuâ€ƒAspâ€ƒGlyâ€ƒLysâ€ƒValâ€ƒAlaâ€ƒIleâ€ƒI #leâ€ƒThrâ€ƒGlyâ€ƒGlyâ€ƒThrâ€ƒLeuâ€ƒâ€ƒ1â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ5â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ10â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ15 Glyâ€ƒIleâ€ƒGlyâ€ƒLeuâ€ƒAlaâ€ƒIleâ€ƒAlaâ€ƒThrâ€ƒLysâ€ƒPheâ€ƒV #alâ€ƒGluâ€ƒGluâ€ƒGlyâ€ƒAlaâ€ƒLysâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ20â€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ25â€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ30 Valâ€ƒMetâ€ƒIleâ€ƒThrâ€ƒGlyâ€ƒArgâ€ƒHisâ€ƒSerâ€ƒAspâ€ƒValâ€ƒG #lyâ€ƒGluâ€ƒLysâ€ƒAlaâ€ƒAlaâ€ƒLysâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ35â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ40â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ45 Serâ€ƒValâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ50  <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ16<211>â€ƒLENGTH:â€ƒ50<212>â€ƒTYPE:â€ƒPRT<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒmutantâ€ƒ1 <400>â€ƒSEQUENCE:â€ƒ16 Serâ€ƒAsnâ€ƒArgâ€ƒLeuâ€ƒAspâ€ƒGlyâ€ƒLysâ€ƒValâ€ƒGlyâ€ƒIleâ€ƒI #leâ€ƒThrâ€ƒGlyâ€ƒGlyâ€ƒThrâ€ƒLeuâ€ƒâ€ƒ1â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ5â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ10â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ15 Glyâ€ƒIleâ€ƒGlyâ€ƒLeuâ€ƒAlaâ€ƒIleâ€ƒAlaâ€ƒThrâ€ƒLysâ€ƒPheâ€ƒV #alâ€ƒGluâ€ƒGluâ€ƒGlyâ€ƒAlaâ€ƒLysâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ20â€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ25â€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ30 Valâ€ƒMetâ€ƒIleâ€ƒThrâ€ƒGlyâ€ƒLeuâ€ƒHisâ€ƒSerâ€ƒAspâ€ƒValâ€ƒG #lyâ€ƒGluâ€ƒIleâ€ƒAlaâ€ƒAlaâ€ƒLysâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ35â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ40â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ45 Serâ€ƒValâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ50  <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ17<211>â€ƒLENGTH:â€ƒ50<212>â€ƒTYPE:â€ƒPRT<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒmutantâ€ƒ1/1 <400>â€ƒSEQUENCE:â€ƒ17 Serâ€ƒAsnâ€ƒArgâ€ƒLeuâ€ƒAspâ€ƒGlyâ€ƒLysâ€ƒValâ€ƒGlyâ€ƒIleâ€ƒI #leâ€ƒThrâ€ƒGlyâ€ƒGlyâ€ƒThrâ€ƒLeuâ€ƒâ€ƒ1â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ5â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ10â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ15 Glyâ€ƒIleâ€ƒGlyâ€ƒLeuâ€ƒAlaâ€ƒIleâ€ƒAlaâ€ƒThrâ€ƒLysâ€ƒPheâ€ƒV #alâ€ƒGluâ€ƒGluâ€ƒGlyâ€ƒAlaâ€ƒLysâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ20â€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ25â€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ30 Valâ€ƒMetâ€ƒIleâ€ƒThrâ€ƒGlyâ€ƒLeuâ€ƒHisâ€ƒSerâ€ƒAspâ€ƒValâ€ƒG #lyâ€ƒGluâ€ƒMetâ€ƒAlaâ€ƒAlaâ€ƒLysâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ35â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ40â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ45 Serâ€ƒValâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ50  <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ18<211>â€ƒLENGTH:â€ƒ50<212>â€ƒTYPE:â€ƒPRT<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒmutantâ€ƒ2 <400>â€ƒSEQUENCE:â€ƒ18 Serâ€ƒAsnâ€ƒArgâ€ƒLeuâ€ƒAspâ€ƒGlyâ€ƒLysâ€ƒValâ€ƒGlyâ€ƒIleâ€ƒI #leâ€ƒThrâ€ƒGlyâ€ƒGlyâ€ƒThrâ€ƒLeuâ€ƒâ€ƒ1â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ5â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ10â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ15 Glyâ€ƒIleâ€ƒGlyâ€ƒLeuâ€ƒAlaâ€ƒIleâ€ƒAlaâ€ƒThrâ€ƒLysâ€ƒPheâ€ƒV #alâ€ƒGluâ€ƒGluâ€ƒGlyâ€ƒAlaâ€ƒLysâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ20â€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ25â€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ30 Valâ€ƒMetâ€ƒIleâ€ƒThrâ€ƒGlyâ€ƒLeuâ€ƒHisâ€ƒSerâ€ƒAspâ€ƒValâ€ƒG #lyâ€ƒGluâ€ƒLysâ€ƒAlaâ€ƒAlaâ€ƒMetâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ35â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ40â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ45 Serâ€ƒValâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ50  <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ19<211>â€ƒLENGTH:â€ƒ50<212>â€ƒTYPE:â€ƒPRT<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒmutantâ€ƒ2/2 <400>â€ƒSEQUENCE:â€ƒ19 Serâ€ƒAsnâ€ƒArgâ€ƒLeuâ€ƒAspâ€ƒGlyâ€ƒLysâ€ƒValâ€ƒGlyâ€ƒIleâ€ƒI #leâ€ƒThrâ€ƒGlyâ€ƒGlyâ€ƒThrâ€ƒLeuâ€ƒâ€ƒ1â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ5â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ10â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ15 Glyâ€ƒIleâ€ƒGlyâ€ƒLeuâ€ƒAlaâ€ƒIleâ€ƒAlaâ€ƒThrâ€ƒLysâ€ƒPheâ€ƒV #alâ€ƒGluâ€ƒGluâ€ƒGlyâ€ƒAlaâ€ƒLysâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ20â€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ25â€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ30 Valâ€ƒMetâ€ƒIleâ€ƒThrâ€ƒGlyâ€ƒLeuâ€ƒLeuâ€ƒSerâ€ƒAspâ€ƒValâ€ƒG #lyâ€ƒGluâ€ƒLysâ€ƒAlaâ€ƒAlaâ€ƒMetâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ35â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ40â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ45 Serâ€ƒValâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ50  <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ20<211>â€ƒLENGTH:â€ƒ50<212>â€ƒTYPE:â€ƒPRT<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒmutantâ€ƒ2/3 <400>â€ƒSEQUENCE:â€ƒ20 Serâ€ƒAsnâ€ƒArgâ€ƒLeuâ€ƒAspâ€ƒGlyâ€ƒLysâ€ƒValâ€ƒGlyâ€ƒIleâ€ƒI #leâ€ƒThrâ€ƒGlyâ€ƒGlyâ€ƒThrâ€ƒLeuâ€ƒâ€ƒ1â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ5â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ10â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ15 Glyâ€ƒIleâ€ƒGlyâ€ƒLeuâ€ƒAlaâ€ƒIleâ€ƒAlaâ€ƒThrâ€ƒLysâ€ƒPheâ€ƒV #alâ€ƒGluâ€ƒGluâ€ƒGlyâ€ƒAlaâ€ƒLysâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ20â€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ25â€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ30 Valâ€ƒMetâ€ƒIleâ€ƒThrâ€ƒAspâ€ƒLeuâ€ƒHisâ€ƒSerâ€ƒAspâ€ƒValâ€ƒG #lyâ€ƒGluâ€ƒLysâ€ƒAlaâ€ƒAlaâ€ƒMetâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ35â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ40â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ45 Serâ€ƒValâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ50  <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ21<211>â€ƒLENGTH:â€ƒ50<212>â€ƒTYPE:â€ƒPRT<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒN-terminalâ€ƒarea <400>â€ƒSEQUENCE:â€ƒ21 Serâ€ƒAsnâ€ƒArgâ€ƒLeuâ€ƒAspâ€ƒGlyâ€ƒLysâ€ƒValâ€ƒAlaâ€ƒIleâ€ƒI #leâ€ƒThrâ€ƒGlyâ€ƒGlyâ€ƒThrâ€ƒLeuâ€ƒâ€ƒ1â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ5â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ10â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ15 Glyâ€ƒIleâ€ƒGlyâ€ƒLeuâ€ƒAlaâ€ƒIleâ€ƒAlaâ€ƒThrâ€ƒLysâ€ƒPheâ€ƒV #alâ€ƒGluâ€ƒGluâ€ƒGlyâ€ƒAlaâ€ƒLysâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ20â€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ25â€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ30 Valâ€ƒMetâ€ƒIleâ€ƒThrâ€ƒGlyâ€ƒArgâ€ƒHisâ€ƒSerâ€ƒAspâ€ƒValâ€ƒG #lyâ€ƒGluâ€ƒLysâ€ƒAlaâ€ƒAlaâ€ƒLysâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ35â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ40â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ45 Serâ€ƒValâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ50  <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ22<211>â€ƒLENGTH:â€ƒ34<212>â€ƒTYPE:â€ƒPRT<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒLBADH <400>â€ƒSEQUENCE:â€ƒ22 Glyâ€ƒIleâ€ƒGlyâ€ƒLeuâ€ƒAlaâ€ƒIleâ€ƒAlaâ€ƒThrâ€ƒLysâ€ƒPheâ€ƒV #alâ€ƒGluâ€ƒGluâ€ƒGlyâ€ƒAlaâ€ƒLysâ€ƒâ€ƒ1â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ5â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ10â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ15 Valâ€ƒMetâ€ƒIleâ€ƒThrâ€ƒGlyâ€ƒArgâ€ƒHisâ€ƒSerâ€ƒAspâ€ƒValâ€ƒG #lyâ€ƒGluâ€ƒLysâ€ƒAlaâ€ƒAlaâ€ƒLysâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ20â€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ25â€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ30 Serâ€ƒVal   SEQâ€ƒIDâ€ƒNOâ€ƒ23<211>â€ƒLENGTH:â€ƒ34<212>â€ƒTYPE:â€ƒPRT<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒsp-Q93761 <400>â€ƒSEQUENCE:â€ƒ23 Glyâ€ƒLeuâ€ƒGlyâ€ƒLysâ€ƒAlaâ€ƒIleâ€ƒAlaâ€ƒThrâ€ƒThrâ€ƒPheâ€ƒA #laâ€ƒHisâ€ƒLeuâ€ƒGlyâ€ƒAlaâ€ƒSerâ€ƒâ€ƒ1â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ5â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ10â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ15 Valâ€ƒAlaâ€ƒIleâ€ƒAlaâ€ƒAlaâ€ƒArgâ€ƒArgâ€ƒLeuâ€ƒAspâ€ƒValâ€ƒL #euâ€ƒGluâ€ƒLysâ€ƒThrâ€ƒAlaâ€ƒAspâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ20â€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ25â€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ30 Gluâ€ƒIle  <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ24<211>â€ƒLENGTH:â€ƒ35<212>â€ƒTYPE:â€ƒPRT<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒsp-P50161 <400>â€ƒSEQUENCE:â€ƒ24 Glyâ€ƒIleâ€ƒGlyâ€ƒAlaâ€ƒAlaâ€ƒIleâ€ƒAlaâ€ƒValâ€ƒAlaâ€ƒLeuâ€ƒG #lyâ€ƒGluâ€ƒArgâ€ƒGlyâ€ƒAlaâ€ƒLysâ€ƒâ€ƒ1â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ5â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ10â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ15 Valâ€ƒValâ€ƒValâ€ƒAsnâ€ƒTyrâ€ƒAlaâ€ƒHisâ€ƒSerâ€ƒArgâ€ƒGluâ€ƒA #laâ€ƒAlaâ€ƒGluâ€ƒLysâ€ƒValâ€ƒValâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ20â€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ25â€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ30 Gluâ€ƒGlnâ€ƒIleâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ35  <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ25<211>â€ƒLENGTH:â€ƒ34<212>â€ƒTYPE:â€ƒPRT<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒsp-O067610 <400>â€ƒSEQUENCE:â€ƒ25 Glyâ€ƒIleâ€ƒGlyâ€ƒArgâ€ƒAlaâ€ƒIleâ€ƒAlaâ€ƒGluâ€ƒLysâ€ƒLeuâ€ƒA #laâ€ƒSerâ€ƒAlaâ€ƒGlyâ€ƒSerâ€ƒThrâ€ƒâ€ƒ1â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ5â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ10â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ15 Valâ€ƒIleâ€ƒIleâ€ƒThrâ€ƒGlyâ€ƒThrâ€ƒSerâ€ƒGlyâ€ƒGluâ€ƒArgâ€ƒA #laâ€ƒLysâ€ƒAlaâ€ƒValâ€ƒAlaâ€ƒGluâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ20â€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ25â€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ30 Gluâ€ƒIle  <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ26<211>â€ƒLENGTH:â€ƒ33<212>â€ƒTYPE:â€ƒPRT<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒsp-P42317 <400>â€ƒSEQUENCE:â€ƒ26 Glyâ€ƒIleâ€ƒArgâ€ƒPheâ€ƒGluâ€ƒIleâ€ƒAlaâ€ƒArgâ€ƒGluâ€ƒPheâ€ƒA #laâ€ƒArgâ€ƒGluâ€ƒGlyâ€ƒAlaâ€ƒSerâ€ƒâ€ƒ1â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ5â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ10â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ15 Valâ€ƒIleâ€ƒValâ€ƒAspâ€ƒLeuâ€ƒArgâ€ƒProâ€ƒGluâ€ƒAlaâ€ƒCysâ€ƒG #luâ€ƒLysâ€ƒAlaâ€ƒAlaâ€ƒSerâ€ƒLysâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ20â€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ25â€ƒâ€ƒâ€ƒâ€ƒâ€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ30 Leu  <210>â€ƒSEQâ€ƒIDâ€ƒNOâ€ƒ27<211>â€ƒLENGTH:â€ƒ18<212>â€ƒTYPE:â€ƒPRT<213>â€ƒORGANISM:â€ƒLactobacillusâ€ƒbrevis<220>â€ƒFEATURE:<223>â€ƒOTHERâ€ƒINFORMATION:â€ƒsp-Q49721 <400>â€ƒSEQUENCE:â€ƒ27 Gluâ€ƒGlyâ€ƒLeuâ€ƒIleâ€ƒGlyâ€ƒArgâ€ƒHisâ€ƒAlaâ€ƒAspâ€ƒValâ€ƒG #lyâ€ƒAlaâ€ƒLysâ€ƒValâ€ƒAlaâ€ƒGlnâ€ƒâ€ƒ1â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ5â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ10â€ƒ#â€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒâ€ƒ15 Leuâ€ƒIle1. An (R)-specific alcohol dehydrogenase with a kcat/Km for NAD+ of 20 to 66 mMâˆ’1secâˆ’1,a) wherein said dehydrogenase is a mutant of a wild-type (R)-specific alcohol dehydrogenase described by the following characteristics: (i) naturally-occurring from Lactobacillus, (ii) having an N-terminal sequence as set forth in SEQ ID NO:15, and (iii) using NADPâˆ’1  as a cofactor for catalytic activity; b) wherein said mutant dehydrogenase has at least one mutation selected from the group consisting of: G37D, R38L, H39L, K45I, K45M, and K48M with respect to SEQ ID NO:15; c) and wherein said mutation or mutations change the alkalinity of the cofactor binding site of the mutant dehydrogenase and promote catalytic activity using NAD+ as a cofactor.