Patent ID: 6309851
Filing Date: 2001-10-30
Classification: C07K,C12N,C12Q

Abstract:
A method for producing a recombinant protein comprising transforming a host bacterium with a first gene encoding a recombinant protein, transforming said host bacterium with a second gene encoding a bacterial periplasmic protein selected from the group consisting of Por, TcpG, and DsbA, and culturing said host bacterium to produce said recombinant protein, which periplasmic protein catalyzes the formation of disulfide bonds between cysteine residues, wherein said host bacterium is selected from the group consisting of E. coli, Haemophilus influenzae, Salmonella typhimurium, Bacillus brevis, Legionella, Erwinia chryantum, Streptococcus, and Staphylococcus.