Patent ID: 8293516
Filing Date: 2012-10-23
Classification: C07K,C12N,C12P

Abstract:
1. A recombinant microorganism produced by transferring a gene encoding a protein or polypeptide of interest to a microorganism strain, wherein the recombinant microorganism is prepared by: inserting, into the genome of a parental microorganism, [i] a transcription initiation regulatory region or [ii] both the transcription initiation regulatory region and a ribosome-binding site, such that said [i] or [ii] is operably linked to the upstream of a inserting, into the genome of a parental microorganism, a gene fragment prepared by operably linking a transcription initiation regulatory region or both the transcription initiation regulatory region and a ribosome-binding site to the upstream of the deleting or inactivating one or more genes selected from the group consisting of an abrB gene, a dltA gene, a dltB gene, a dltC gene, a dltD gene, and a dltE gene.