Patent ID: 7939259
Filing Date: 2011-05-10
Classification: C12Q

Abstract:
1. A method for sequencing a target nucleic acid, comprising: a) providing a daughter strand produced by a template-directed synthesis, the daughter strand comprising a plurality of subunits coupled in a sequence corresponding to a contiguous nucleotide sequence of all or a portion of the target nucleic acid, wherein the daughter strand is formed from a plurality of oligomer or monomer substrate constructs, wherein the individual subunits of the daughter strand comprise a tether, at least one probe or nucleobase residue, and at least one selectively cleavable bond, and wherein the tether, upon cleavage of the at least one selectively cleavable bond, permits lengthening of the subunits of the daughter strand; b) cleaving the at least one secletively caeavable bond to yield an Xpandomer of a length longer than the plurality of the subunits of daughter strand, the Xpandomer comprising the tethers and reporter elements for parsing genetic information in a sequence corresponding to the contiguous nucleotide sequence of all or a portion of the target nucleic acid; and c) detecting the reporter elements of the Xpandomer.