Patent ID: 6800462
Filing Date: 2004-10-05
Classification: C07K,G01N

Abstract:
A method of generating an antibody, the method comprising:administering to a first mammal a nucleic acid encoding a fusion protein and expressing the fusion protein in the first mammal, wherein the fusion protein contains a first amino acid sequence and a second amino acid sequence, wherein the second amino acid sequence contains a first member of a specific binding pair, and wherein the nucleic acid is administered to the first mammal intravenously, intramuscularly, intraarterially, intradermally, intraperitoneally, intranasally, or subcutaneously; removing from the first mammal a biological sample that contains the fusion protein; binding a second member of the specific binding pair to the fusion protein via the first member of the specific binding pair to thereby isolate the fusion protein from the biological sample; and administering to a second mammal the fusion protein or a portion thereof comprising the first amino acid sequence, to thereby generate an antibody response in the second mammal against the first amino acid sequence of the fusion protein.