Patent ID: 8178349
Filing Date: 2012-05-15
Classification: A61P,C12N

Abstract:
1. A method of differentiating primate embryonic stem cells into a substantially pure homogeneous population of neural precursor cells comprising: (a) obtaining a primate embryonic stem cell culture, (b) propagating the stem cells on feeder cells one or more days to form embryonic stem cell clusters, (c) triturating the stem cell clusters (d) washing the triturated stem cell clusters at least once in a physiological medium, (e) forming the stem cell clusters into spheroid bodies in suspension medium by incubating the stem cell clusters in serum free medium supplemented with N2, B27, fibroblast growth factor 2, epithelial growth factor, and N-acetyl-cysteine, and (f) culturing the spheroid bodies in an expansion medium containing serum or a serum substitute, fibroblast growth factor 2, epithelial growth factor, and 2 mM N-acetyl-cysteine, wherein a substantially pure homogeneous population of neural precursor cells that is at least 90% pure is generated.