Patent ID: 6342355
Filing Date: 2002-01-29
Classification: C12Q

Abstract:
A method of analyzing a nucleic acid in a target sample, comprising:(a) hybridizing a first-labelled control sample comprising a homozygous reference allele, and a second-labelled target sample comprising the homozygous reference allele, or variant alleles differing from the reference allele at a locus, or one variant allele differing from the reference allele at the locus and one reference allele, to at least one set of probes spanning the locus and complementary to the reference allele, wherein the first and second labels are different; (b) measuring the intensity of first and second label bound to probes in the probe set; and (c) determining a normalized intensity ratio of first label to second label for probes in the probe set, wherein the value of the ratio relative to one or more threshold values is an indicator of whether only reference alleles, only variant alleles or both reference and variant alleles are present in the target sample.