Patent ID: 6541248
Filing Date: 2003-04-01
Classification: A61P,C12N

Abstract:
A lentiviral vector system comprising:(i) a lentiviral vector genome comprising at least one first nucleotide sequence encoding an anti-sense ribonucleic acid or a ribozyme, wherein said sequence is capable of binding to and effecting the cleavage of a second nucleotide sequence, or transcription product thereof, said second nucleotide sequence encoding a lentiviral polypeptide required for the assembly of lentiviral particles; and (ii) at least one third nucleotide sequence encoding said lentiviral polypeptide required for the assembly of the lentiviral genome into viral particles, which at least one third nucleotide sequence has a different nucleotide sequence to the second nucleotide sequence such that said third nucleotide sequence, or transcription sequence, or transcription product thereof, is codon-optimized and resistant to cleavage directed by said anti-sense ribonucleic acid or said ribozyme.