Patent ID: 6017739
Filing Date: 2000-01-25
Classification: C12N,C12Q

Abstract:
A method for selectively concentrating a first nucleic acid from a sample containing said first nucleic acid and at least a second nucleic acid, wherein said first nucleic acid comprises a particular segment of a target sequence, said target sequence of said first nucleic acid containing a mutant sequence, and said second nucleic acid comprises the particular segment of said target sequence, with the exception that the target sequence of said second nucleic acid does not contain said mutant sequence, said method comprising the following steps:(1) preparing a nucleic acid sample by amplifying said particular segment of said target sequence of said first and second nucleic acids,(2) adding to said nucleic acid sample an equimolar amount or more of a labeled nucleic acid standard prepared by incorporating a label capable of binding to a solid support into a nucleic acid having a nucleotide sequence complementary to the particular segment of said second nucleic acid,(3) performing a competitive hybridization after said adding step (2), and(4) separating and removing a hybridizate having said label capable of binding to the solid support and the residual labeled nucleic acid standard in the reaction solution after the competitive hybridization by trapping said hybridizate having said label and the residual labeled nucleic acid standard on the solid support, wherein,(i) steps (1) to (4) are sequentially performed one or more times, or(ii) steps (1) to (4) are sequentially performed once and then steps (2) to (4) are sequentially performed one or more times.