Patent ID: 7087719
Filing Date: 2006-08-08
Classification: A61P,C07K,Y10S

Abstract:
1. A method of producing crystalline human albumin comprising: a) concentrating an albumin containing fluid until said fluid has at least 15 grams of albumin per liter of solution; b) adding a first phosphate mixture to said albumin containing fluid until the concentration of said phosphate mixture is in the range of 2.4–2.6 molar; c) providing a first filtering of said albumin containing solution so as to form a resultant crystallizing batch solution to remove impurities; d) cooling the resultant filtrate of said crystallizing batch solution to a temperature of at most 15° C.; e) allowing the human albumin in said crystallizing batch solution to crystallize; f) adding more of said first phosphate mixture to said crystallizing batch solution sufficient to achieve a concentration of at most 3.0 molar; g) making a first separation of albumin crystals from any remaining fluid; h) suspending the albumin crystals from said first separation of albumin crystals in a second phosphate mixture wherein said second phosphate mixture has a concentration of 2.7–3.0 M; i) heating the crystal suspension from said first separation of albumin crystals up to temperature in the range of 40–50° C. in order to dissolve the crystals, j) providing a second filtering to the dissolved crystal suspension from said first separation of albumin crystals; k) cooling the resultant dissolved crystal suspension from said first separation of albumin crystals to a temperature of at most 15° C.; and l) allowing albumin crystals to form the resultant cooled crystal suspension; wherein the application of these specific steps allows the purification and crystallization of human albumin from a given human albumin containing feedstream.