Patent ID: 8404443
Filing Date: 2013-03-26
Classification: C12Q

Abstract:
1. A reaction mixture that is at a temperature below 37° C., said mixture comprising a template nucleic acid, a primer having a nucleotide sequence that is complementary to a specific target portion of said template nucleic acid, an enzyme effective to catalyze primer extension, nucleotides necessary for enzyme-directed nucleic acid synthesis, and a single-stranded nucleic acid binding protein having no known enzymatic activity and that is effective to inhibit said primer from participating in a primer extension reaction up to at least a first temperature at or below 30° C., wherein the inhibitive capability of said single-stranded nucleic acid binding protein is lost at an inactivation temperature in the range of 50° C. to about 72° C. as a result of being subjected to said inactivation temperature, said template nucleic acid being unamplified and not denatured.