Patent ID: 8936908
Filing Date: 2015-01-20
Classification: C12N,C12P

Abstract:
1. A method for the production of a permanent human cell line comprising: (a) transfecting isolated primary human cells with a nucleic acid molecule or at least two different nucleic acid molecules, wherein in the case that one nucleic acid molecule is transfected, said nucleic acid molecule comprises a sequence or sequences encoding at least one cell transforming factor and an antibody, and wherein in the case that at least two different nucleic acid molecules are transfected, a first nucleic acid molecule comprises a sequence encoding at least one cell transforming factor, and a second nucleic acid molecule comprises a sequence encoding at least one antibody, wherein transfecting said first and second nucleic acid sequences occurs simultaneously; (b) selecting said permanent human cell line expressing the antibody, wherein the selection is based on expression of said transforming factor and without any additional exogenous chemical selection.