Patent ID: 7851152
Filing Date: 2010-12-14
Classification: C12Q

Abstract:
1. A method of detecting the structures and interactions of a nucleic acid molecule, comprising: placing in said nucleic acid molecule or substituting corresponding nucleotide(s) of said nucleic acid molecule with one or multiple fluorescent nucleotide analogue(s); introducing so modified nucleic acid molecule into a target matrix or medium so as to subject the nucleic acid molecule to interactions with components in the matrix or medium; and detecting the structures and activities of said nucleic acid molecule by measuring fluorescence properties of the fluorescent base analogue(s) in said nucleic acid molecule at the fluorescent base analogue's respective emission wavelength by indirect excitation of nearby natural bases of said nucleic acid molecule at a wavelength in the range of 240 nm to 280 nm, in which the fluorescent base analogue is 2-aminopurine, and the emission wavelength is in the range of 350 nm to 380 nm.