Patent ID: 6784285
Filing Date: 2004-08-31
Classification: C07K,C30B,Y02A

Abstract:
A method for assaying a candidate compound for its ability to interact with a modified vascular endothelial growth factor receptor-2 (VEGFR-2) receptor polypeptide comprising:(a) expressing an isolated DNA sequence or variants thereof encoding the modified VEGFR-2 gene construct wherein said VEGFR-2 gene contains a synthetic catalytic linker between helices &agr;D and &agr;E, wherein said linker comprises at least one amino acid from the kinase insert domain (KID) of the VEGFR-2 gene catalytic region, in a host that will transcribe the DNA sequence and produce a form of the modified polypeptide which form may be assayed for interaction of said polypeptide with said candidate compound; (b) exposing said modified polypeptide to said candidate compound; and (c) evaluating the interaction of said modified polypeptide with said candidate compound.