Patent ID: 6297026
Filing Date: 2001-10-02
Classification: A61K,C07K,G01N

Abstract:
An isolated nucleic acid molecule selected from the group consisting of:(a) a nucleic acid molecule which hybridizes under stringent conditions with a nucleic acid molecule complimentary to SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:60, SEQ ID NO:62, nucleotides 232 to 1416 of SEQ ID NO:1, nucleotides 56-1249 of SEQ ID NO:3, nucleotides 73-1269 of SEQ ID NO:60 or nucleotides 50-1240 of SEQ ID NO:62; and which encodes a C140 receptor which can produce agonist-induced increases in Ca.sup.+2 release when expressed in Xenopus laevis oocytes, wherein the stringent conditions are: (1) hybridization in 50% (vol/vol) formamide with 0.1% bovine serum albumin, 0.1% Ficoll, 0.1% polyvinylpyrrolidone, 50 mM sodium phosphate buffer at pH 6.5 with 750 mM NaCl, 75 mM sodium citrate at 42.degree. C., (z) hybridization in 50% formamide, 5.times.SSC (0.75 M NaCl, 0.075 M sodium citrate), 50 mM sodium phosphate (pH 6.8), 0.1% sodium pyrophosphate, 5.times.Denhardt's solution, sonicated salmon sperm DNA (50 .mu.g/ml), 0.1% SDS, and 10% dextran sulfate at 42.degree. C.; with washes at 42.degree. C. in 0.2.times.SSC and 0.1% SDS or washes with 0.015 M NaCl, 0.0015 M sodium citrate, 0.1% NaDodSO.sub.4 at 50.degree. C.;(b) a nucleic acid molecule that is filly complementary to a nucleic acid molecule comprising SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:60, SEQ ID NO:62, nucleotides 232 to 1416 of SEQ ID NO:1, nucleotides 56-1249 of SEQ ID NO:3, nucleotides 73-1269 of SEQ ID NO:60 or nucleotides 50-1240 of SEQ ID NO:62; and(c) a nucleic acid molecule which encodes a protein comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:61 or SEQ ID NO:63.