Patent ID: 8252531
Filing Date: 2012-08-28
Classification: C12Q

Abstract:
1. A method of clinical analysis comprising the steps of: (1) contacting a sample containing a specific nucleic substance with a test agent capable of hybridizing with said specific nucleic substance in the presence of polymer (H) having a nonspecific hybridization inhibitory action in an amount of 0.0001 to 20 wt % of a hybridization system, under particular conditions for hybridizing said specific nucleic substance with said test agent, and specifically hybridizing said nucleic substance with said test agent, while said polymer (H) inhibits nonspecific hybridization, wherein said polymer (H) has a weight average molecular weight of 1000 to 5000000 and has a group derived from a monomer having a phosphorylcholine-like group represented by the formula (1): wherein X stands for a divalent organic residue, Y stands for an alkyleneoxy group having 1 to 6 carbon atoms, Z stands for a hydrogen atom or R 5 O(C═O)—, provided that R 5 stands for an alkyl or hydroxyalkyl group having 1 to 10 carbon atoms; R 1 stands for a hydrogen atom or a methyl group, R 2 , R 3 , and R 4 are the same or different groups, and each stands for a hydrogen atom, or an alkyl or hydroxyalkyl group having 1 to 6 carbon atoms; m is 0 or 1, and n denotes an integer of 1 to 4, and (2) detecting a reactant generated by hybridization of said specific nucleic substance with said test agent in step (1).