Patent ID: 6780596
Filing Date: 2004-08-24
Classification: G01N

Abstract:
A method for determining whether a plant extract has bioloaical activity, which method comprises incubating the plant extract with cultured mammalian cells to produce tested mammalian cells, lysing said tested mammalian cells to produce a mixture of cellular proteins, electrophoretically-separating said mixture of cellular proteins, reacting said mixture of cellular proteins with a labeled monoclonal or polyclonal antibody directed against phosphorylated amino acids and comparing the pattern of phosphorylation produced by five or more cellular proteins of said mixture of cellular proteins in said tested mammalian cells to the pattern of phosphorylation produced by five or more cellular proteins of a mixture of cellular proteins in control cells; wherein said control cells are said cultured mammalian cells which have not been exposed to said plant extract, and wherein differences in said pattern of phosphorylation produced in said tested mammalian cells as compared to said pattern of phosphorylation produced in said control cells indicates biological activity of said plant extract.