Patent ID: 9090924
Filing Date: 2015-07-28
Classification: C12N,C12P,C12Y

Abstract:
1. A method for producing pyripyropenes comprising culturing a transformed host cell obtained by transforming a heterologous host cell with a heterologous nucleic acid construct and collecting pyripyropenes from the culture, wherein said heterologous nucleic acid construct comprises a pyripyropene biosynthetic gene cluster and a marker gene simultaneously or separately, and wherein said pyripyropene biosynthetic gene cluster comprises at least one nucleotide sequence selected from (I) to (IV) below: (I) the nucleotide sequence of positions 2911 to 27797 in the nucleotide sequence set forth in SEQ ID NO: 266; (II) a nucleotide sequence which is capable of hybridizing with every nucleotide in the full length complementary sequence of positions 2911 to 27797 in SEQ ID NO: 266 under stringent conditions wherein said stringent conditions comprise washing with 2× saline-sodium citrate (SSC) buffer and 0.5% sodium dodecyl sulfate (SDS) at 60° C. for 20 minutes, and then washing with 0.2×SSC and 0.1% SDS at 60° C. for 15 minutes, III) a nucleotide sequence comprising positions 2911 to 27797 of the nucleotide sequence set forth in SEQ ID NO: 266 having no more than 10 nucleotides deleted, substituted, inserted, or added in each of the nucleotide sequences corresponding to positions 3342 to 5158, 5382 to 12777, 13266 to 15144, 16220 to 18018, 18506 to 19296, 19779 to 21389, 21793 to 22877, 23205 to 24773, and 258124 to 27178 of the nucleotide sequence set forth in SEQ ID NO: 266, IV) a nucleotide sequence comprising at least 95% identity to each of the nucleotide sequences corresponding to positions 3342 to 5158, 5382 to 12777, 13266 to 15144, 16220 to 18018, 18506 to 19296, 19779 to 21389, 21793 to 22877, 23205 to 24773, and 258124 to 27178 of the nucleotide sequence set forth in SEQ ID NO: 266