Patent ID: 7189548
Filing Date: 2007-03-13
Classification: C12N

Abstract:
1. A modified sarcosine oxidase wherein said enzyme (a) hydrolyzes 1 mol of sarcosine to produce 1 mol of glycine and 1 mol of formaldehyde; (b) has an optimal pH of about 6.5; (c) does not change its activity after being incubated at pH of between 6.0 and 11.0 for 5 hours at 25° C.; (d) has an optimal temperature of 60° C.; (e) is stable after heating for 10 minutes in 50 mM potassium phosphate buffer and at pH 7.5, at about 50° C.; (f) retains 90% of activity after 5 hours at pH 6.0 and 25° C. and it retains 70% activity after 17 hours at pH 6.0 and 25° C.; (g) has a molecular weight of approximately 43,000 (SDS-PAGE); and (h) has Km value of 5.9 mM at pH 6.5; and wherein said modified sarcosine oxidase is encoded by a DNA derived by modifying a DNA having the nucleotide sequence represented by SEQ ID NO:2.