Patent ID: 8357524
Filing Date: 2013-01-22
Classification: C12N

Abstract:
1. A method of producing a chemically modified mutated subtilisin that catalyzes a transamidation or a transpeptidation or a transesterification reaction comprising replacing one or more amino acids at positions selected from the group of positions, position 62 in the S replacing the thiol hydrogen of said one or more replacing cysteine residues with a substituent moiety selected from the group of substituents consisting of (R)-2-methoxy-2-phenyl-ethylmethanethiosulfonate, (S)-2-methoxy-2-phenyl-ethylmethanethiosulfonate, (R)-2-hydroxy-2-phenyl-ethylmethanethiosulfonate, (S)-2-hydroxy-2-phenyl-ethylmethanethiosulfonate, N-(3′-methanethiosulfonatopropyl)-2-oxazoldinone, N-(3′-methanethiosulfonatopropyl)-(R)-4-isopropyl-2-oxazolidinone, N-(3′-methanethiosulfonatopropyl)-(S)-4-isopropyl-2-oxazolidinone, N-(3′-methanethiosulfonatopropyl)-(R)-4-phenyl-2-oxazolidinone, N-(3′-methanethiosulfonatopropyl)-(S)-4-phenyl-2-oxazolidinone, N-(3′-methanethiosulfonatopropyl)-(R)-4-benzyl-2-oxazolidinone, N-(3′-methanethiosulfonatopropyl)-(S)-4-benzyl-2-oxazolidinone, N-(2′-methanethiosulfonatoethyl)-(R)-4-phenyl-2-oxazolidinone, N-(2′-methanethiosulfonatoethyl)-(S)-4-phenyl-2-oxazolidinone, N-(2′-methanethiosulfonatopropyl)-(R)-4-benzyl-2-oxazolidinone, N-(2′-methanethiosulfonatopropyl)-(S)-4-benzyl-2-oxazolidinone, N-(3′-methanethiosulfonatopropyl)-(3aR-cis)-3,3a,8,8a-tetrahydro-2H-indeno[1,2-d]-oxazol-2-one and N-(3′-methanethiosulfonatopropyl)-(3aS-cis)-3,3a,8,8a-tetrahydro-2H-indeno[1,2-d]-oxazol-2-one.