Patent ID: 6646185
Filing Date: 2003-11-11
Classification: C12N

Abstract:
A method for producing a genetically transformed zoysiagrass comprising the steps of:i) inducing and growing the calli obtained from seed of a zoysiagrass on MS medium containing 3% sucrose, 100 mg/L alpha-ketoglutaric acid, 4 mg/L thiamine-HCl, 2 mg/L 2,4-D, 0.2 mg/L BA, and 0.2% GELRITE; ii) infecting the calli of the zoysiagrass with Agrobacterium cells to introduce a bialaphos resistance bar DNA coding sequence to the calli of zoysiagrass; iii) co-cultivating the calli of the zoysiagrass and Agrobacterium cells in a co-cultivation medium containing acetosyringone and lacking both 2,4-dichlorophenoxyacetic acid and calcium; iv) eliminating the Agrobacterium cells from the co-cultivation medium; and v) regenerating the calli into transgenic zoysiagrass.