Patent ID: 6866997
Filing Date: 2005-03-15
Classification: C07K,C12N,C40B

Abstract:
1. A method for preparing a nucleic acid binding protein that binds to a target nucleotide sequence, wherein the binding protein comprises a plurality of zinc fingers of the Cys2-His2 class, wherein the method comprises: i) selecting a quadruplet within the target nucleotide sequence; ii) designing the binding protein such that binding of a zinc finger to the quadruplet is obtained by choosing the sequence of particular residues of the zinc finger depending on the nucleotide sequence of the quadruplet, as follows: iii) synthesizing a polynucleotide encoding the binding protein of (ii); iv) introducing the polynucleotide of (iii) into a cell; and v) incubating the cell under conditions in which the encoded nucleic acid binding protein is expressed.