Patent ID: 9090916
Filing Date: 2015-07-28
Classification: C12P,Y02E

Abstract:
1. A method of producing a lignocellulosic hydrolyzate (sweet juice) for alcoholic fermentation from a lignocellulosic substrate by enzymatic hydrolysis, comprising (a) a lignocellulose conversion stage, comprising (1) contacting the lignocellulosic substrate with a cellulase solution in a first reactor to produce a first hydrolyzate, wherein said lignocellosic substrate has been pretreated to improve the susceptibility of the substrate to enzymatic hydrolysis; (2) sending the thus-produced first hydrolyzate obtained at the outlet of said first reactor to a separation means, whereby cellulases in the first hydrolyzate are extracted from the first hydrolyzate and retained by the separation means for return to the first reactor, to produce a cellulase-free first hydrolyzate; (3) sending the cellulase-free first hydrolyzate to a second reactor comprising immobilized β-glucosidases, wherein said second reactor is separate from said first lignocellulose enzymatic hydrolysis reactor, to produce a second hydrolyzate, and separating the second hydrolyzate from the immobilized β-glucosidases; (b) a sweet juice production stage, comprising (c) a reactor emptying stage, comprising completely emptying the first and second reactors.