Patent ID: 6171785
Filing Date: 2001-01-09
Classification: B01L,C12Q,G01N,Y10S,Y10T

Abstract:
A method for detecting the presence of a target nucleic acid in a sample by amplification, said method comprising:(a) providing a nucleic acid amplification reaction mixture that comprises said sample and a fluorescent dye, whose fluorescence intensity is increased upon intercalation into a double-stranded nucleic acid, wherein said reaction mixture is contained within a container which container is operably linked to a device suitable for inputting excitation light into said reaction mixture and conveying fluorescent light emitted by said reaction mixture to a detector;(b) measuring said emitted light produced by the mixture of step (a);(c) treating said mixture under conditions for amplifying said target nucleic acid to produce amplified double-stranded nucleic acid;(d) measuring said emitted light produced by said mixture of step (c); and(e) determining if amplification has occurred.