Patent ID: 8673312
Filing Date: 2014-03-18
Classification: C07K

Abstract:
1. A method for diethylaminoethyl (DEAE) chromatography purification of recombinant Helicobacter pylori neutrophil-activating protein (HP-NAP), consisting the steps of: providing a sample containing recombinant HP-NAP without precipitating in ammonium sulfate, wherein the sample is obtained from a protein expression system that can express recombinant HP-NAP; purifying the sample by passing through one DEAE resin one time between range pH 8 and pH 9; and collecting a unbound fraction and a wash fraction obtained through above step, wherein the unbound fraction and the wash fraction contain the recombinant HP-NAP having a purity of at least 90% without passing through another resin.