Patent ID: 7452680
Filing Date: 2008-11-18
Classification: A61K,A61P,G01N

Abstract:
1. A method of screening an agent, conjugate or conjugate moiety for capacity to be transported through the blood brain barrier, comprising: (a) providing a cell expressing the MCT1 transporter, wherein: a nucleic acid molecule encoding the MCT1 transporter has been transfected or injected into the cell; and the MCT1 transporter is the protein encoded by SEQ ID NO: 1 or has at least 90% sequence identity to the protein encoded by SEQ ID NO:1, and the MCT1 transporter can transport lactic acid, the MCT1 transporter being situated in the plasma membrane of the cell; (b) contacting the cell with an agent, conjugate or conjugate moiety in vitro; and (c) determining whether the agent, conjugate or conjugate moiety passes through the plasma membrane via the MCT1 transporter, passage through the plasma membrane via the MCT1 transporter indicating the agent, conjugate or conjugate moiety has capacity to be transported through the blood brain barrier; wherein: