Patent ID: 9074228
Filing Date: 2015-07-07
Classification: C12N,C12P,C12Y

Abstract:
1. A process for the preparation of an optically active chiral amine and pyruvate as alpha-ketone by-product comprising: a) providing an amino acceptor and alanine as an amino donor; b) reacting the amino acceptor and the amino donor with a (R)- or (S)-selective transaminase; c) obtaining the desired optically active chiral amine and pyruvate as alpha-ketone by-product; and d) removing pyruvate from the reaction mixture with a decarboxylase to shift the equilibrium to the product side, wherein the optically active chiral amine is selected from the group consisting of cephalosporine, heterocyclic boronic acids, L-dihydroxyphenylalanine (L-Dopa), α-methyldopa, D-phenylglycine, β-hydroxyphenylglycine, phenylalanine, alanine, 3-aminopiperidine, alkyl-3-amino-butanoates, 3-aminopyrrolidine (3-AP), 3-pyridyl-1-ethylamine (3-PEA), N-1-boc-3-aminopyrrolidine (B3AP), 3-aminobutyric acid ethyl ester (3-ABEE), 3-aminopentanoic acid methyl ester (3-APME), α-methyl-4-(3-pyridyl)-butanamine, glutamate, phosphinothricine, β-aminobutyrate, sec-butylamine, methoxyisopropylamine, and 1-N-boc-3-aminopiperidine.