Patent ID: 7226742
Filing Date: 2007-06-05
Classification: C12Q

Abstract:
1. A method for detecting a mutation, comprising: a) amplifying a target polynucleotide using a forward primer and a reverse primer to produce an amplified target polynucleotide; b) contacting said amplified target polynucleotide with two or more restriction endonucleases to generate at least one restriction fragment, said restriction fragment comprising two single-stranded fragments of 2–32 nucleotides each, wherein both of said single-stranded fragments contains at least one mutation sequence, said mutation sequence being a base substitution, deletion or insertion; c) measuring the molecular weights of said single-stranded fragments, and d) comparing said molecular weights of said single-stranded fragments to the molecular weights of control fragments, wherein if said molecular weights of said single-stranded fragments differs from the molecular weights of said control fragments, said mutation is detected.