Patent ID: 7833716
Filing Date: 2010-11-16
Classification: C12N,C12Q

Abstract:
1. A method for the selective amplification of at least one target nucleic acid sequence from a nucleic acid sample, the method comprising the steps of: (a) treating a target nucleic acid sequence in a nucleic acid sample with a tagged oligonucleotide comprising a heterologous tag sequence, wherein hybridization of the tagged oligonucleotide to the target nucleic acid sequence produces a tagged target nucleic acid sequence; (b) reducing in the sample the effective concentration of the tagged oligonucleotides that have not hybridized to a target nucleic acid sequence to produce a tagged target nucleic acid sequence and that are in a form capable of hybridizing to a target nucleic acid sequence, wherein said reducing is performed prior to an extension reaction; and (c) subjecting the tagged target nucleic acid sequence to reagents and conditions sufficient for detectable amplification of the target nucleic acid sequence, wherein the subjecting step exposes the nucleic acid sample to a known contaminating source of the target nucleic acid sequence after step (b), the subjecting step comprising; and wherein detectable amplification of the target nucleic acid sequence is substantially limited to amplification of target nucleic acid sequence contributed by the tagged target nucleic acid sequence of step (a) and not by the target nucleic acid sequence contributed by the known contaminating source.