Patent ID: 6248597
Filing Date: 2001-06-19
Classification: G01N,Y10S

Abstract:
An agglutination assay for determining the amount of an analyte in a sample, said assay comprising:a. combining said sample with a mixture of microparticles, said mixture comprising first microparticles having a mean diameter and a refractive index, wherein said first microparticles are coated with a first binding partner for said analyte, and second microparticles having a mean diameter and a refractive index, wherein said second microparticles are coated with a second binding partner for said analyte, and said first microparticles having stronger light scattering properties than said second microparticles, and said first binding partner coated upon said first microparticles having a higher reactivity for said analyte than said second binding partner coated upon said second microparticles,b. measuring light scattering of said first microparticles caused by agglutination of said first coated microparticles with said analyte and measuring light scattering of said second microparticles caused by agglutination of said second coated microparticles with said analyte, andc. obtaining a change between said light scattering of said first microparticles caused by agglutination of said first coated microparticles with said analyte and said light scattering of said second microparticles caused by agglutination of said second coated microparticles with said analyte, andd. comparing said chance with a calibration curve.