Patent ID: 6194142
Filing Date: 2001-02-27
Classification: A61K,A61P,C07K,C12N,C12Q,G01N,Y10S

Abstract:
A polypeptide fragment of a viral protein encoded by a nucleotide sequence from a viral genome selected from the group consisting of HIV-1, HIV-2, and SIV and expressed by a method comprising:a) amplifying the nucleic acid encoding said polypeptide with at least two primers, wherein said first primer is complementary to a region of nucleotides of the nucleic acid of said genome, said second primer is complementary to a region of nucleotides of the strand of DNA complementary to said nucleic acid of said genome, wherein said regions of nucleotides are separated by about 100 to about 1100 base pairs when said complementary strands are hybridized to form one double-stranded nucleic acid, and said primers are selected from the group of nucleotides oriented in the 5' to 3' direction consisting of:nucleotides 636-653, 854-872, 1369-1388, and 2021-2039 of the gag gene of HIV-1 Bru;nucleotides 900-881, 1385-1369, 1388-1369, and 2039-2021 of a nucleic acid sequence complementary to the gag gene of HIV-1 Bru;nucleotides 635-652, 864-888, 1403-1421, and 2055-2073 of the gag gene of HIV-1 Mal;nucleotides 916-897, 1419-1403, 1421-1403, and 2073-2055 of a nucleic acid sequence complementary to the gag gene of HIV-1 Mal;nucleotides 636-653, 848-872, 1369-1388, and 2024-2042 of the gag gene of HIV-1 Eli;nucleotides 900-881, 1385-1369, 1388-1369, and 2042-2024 of a nucleic acid sequence complementary the gag gene of HIV-1 Eli;nucleotides 859-876, 1160-1184, 1687-1706, and 2329-2349 of the gag gene of HIV-2 ROD;nucleotides 1212-1193, 1703-1687, 1706-1687, and 2349-2329 of a nucleic acid sequence complementary to the gag gene of HIV-2 ROD;nucleotides 834-851, 1124-1148, 1651-1670, and 2299-2318 of the gag gene of SIV-MAC;nucleotides 1176-1157, 1667-1651, 1670-1651, and 2381-2299 of a nucleic acid sequence complementary to the gag gene of SIV-MAC;nucleotides 5590-5610 of the vpr gene of HIV-1 Bru;nucleotides 5870-5849 of a nucleic acid sequence complementary to the vpr gene of HIV-1 Bru;nucleotides 5585-5605 of the vpr gene of HIV-1 Mal;nucleotides 5865-5844 of a nucleic acid sequence complementary to the vpr gene of HIV-1 Mal;nucleotides 5554-5574 of the vpr gene of HIV-1 Eli;nucleotides 5834-5813 of a nucleic acid sequence complementary to the vpr gene of HIV-1 Eli;nucleotides 6233-6296 of the vpr gene of HIV-2 ROD;nucleotides 6551-6531 of a nucleic acid sequence complementary to the vpr gene of HIV-2 ROD;nucleotides 6147-6170 of the vpr gene of SIV-MAC;nucleotides 6454-6431 of a nucleic acid sequence complementary to the vpr gene of SIV-MAC;nucleotides 2620-2643, 3339-3361, 4186-4207, and 4992-5011 of the pol gene of HIV-1 Bru;nucleotides 2643-2620, 3361-3339, 4207-4186, and 5011-4992 of a nucleic acid sequence complementary to the pol gene of HIV-1 Bru;nucleotides 2615-2638, 3333-3356, 4181-4202, and 4987-5006 of the pol gene of HIV-1 Mal;nucleotides 2638-2615, 3356-3334, 4202-4181, and 5006-4987 of a nucleic acid sequence complementary to the pol gene of HIV-1 Mal;nucleotides 2584-2607, 3303-3325, 4150-4171, and 4956-4975 of the pol gene of HIV-1 Eli;nucleotides 2607-2584, 3325-3303, 4171-4150, and 4975-4956 of a nucleic acid sequence complementary to the pol gene of HIV-1 Eli;nucleotides 2971-2994, 3690-3712, 4534-4555, and 5340-5359 of the pol gene of HIV-2 ROD;nucleotides 2994-2971, 3712-3690, 4555-4534, and 5359-5340 of a nucleic acid sequence complementary to the pol gene of HIV-2 ROD;nucleotides 2887-3010, 3606-3628, 4450-4471, and 5256-5275 of the pol gene of SIV-MAC;nucleotides 3010-2887, 3628-3606, 4471-4450, and 5275-5256 of a nucleic acid sequence complementary to the pol gene of SIV-MAC;nucleotides 9165-9185 and 9542-9564 of the nef2 gene of HIV-2 ROD;nucleotides 9564-9542 and 9956-9933 of a nucleic acid sequence complementary to the nef2 gene of HIV-2 ROD;nucleotides 9139-9159 and 9516-9538 of the nef2 gene of SIV-MAC;nucleotides 9538-9516 and 9893-9870 of a nucleic acid sequence complementary to the nef2 gene of SIV-MAC;nucleotides 5424-5450 and 5754-5775 of the vif2 gene of HIV-2 ROD;nucleotides 5775-5754 and 6082-6061 of a nucleic acid sequence complementary to the vif2 gene of HIV-2 ROD;nucleotides 5340-5366 and 5670-5691 of the vif2 gene of SIV-MAC;nucleotides 5691-5670 and 5995-5974 of a nucleic acid sequence complementary to the vif2 gene of SIV-MAC;nucleotides 5900-5918 of the vpx gene of HIV-2 ROD;nucleotides 6228-6208 of a nucleic acid sequence complementary to the vpx gene of HIV-2 ROD;nucleotides 5813-5831 of the vpx gene of SIV-MAC;nucleotides 6141-6121 of a nucleic acid sequence complementary to the vpx gene of SIV-MAC;nucleotides 9116-9136 of the nef1 gene of HIV-1 Bru;nucleotides 9136-9116 and 9503-9483 of a nucleic acid sequence complementary to the nef1 gene of HIV-1 Bru;nucleotides 9117-9137 of the nef1 gene of HIV-1 Mal;nucleotides 9137-9117 and 9505-9484 of a nucleic acid sequence complementary to the nef1 gene of HIV-1 Mal;nucleotides 9062-9082 of the nef1 gene of HIV-1 Eli;nucleotides 9082-9062 and 9449-9428 of a nucleic acid sequence complementary to the nef1 gene of HIV-1 Eli;nucleotides 5073-5099 and 5383-5405 of the vif1 gene of HIV-1 Bru;nucleotides 5405-5383 and 5675-5653 of a nucleic acid sequence complementary to the vif1 gene of HIV-1 Bru;nucleotides 5068-5094 and 5378-5400 of the vif1 gene of HIV-1 Mal;nucleotides 5400-5378 and 5670-5648 of a nucleic acid sequence complementary to the vif1 gene of HIV-1 Mal;nucleotides 5037-5063 and 5347-5369 of the vif1 gene of HIV-1 Eli;nucleotides 5369-5347 and 5639-5617 of a nucleic acid sequence complementary to the vif1 gene of HIV-1 Eli;nucleotides 6081-6105 and 6240-6263 of the vpu gene of HIV-1 Bru;nucleotides 6343-6321 of a nucleic acid sequence complementary to the vpu gene of HIV-1 Bru;nucleotides 6076-6100 and 6238-6261 of the vpu gene of HIV-1 Mal;nucleotides 6338-6316 of a nucleic acid sequence complementary to the vpu gene of HIV-1 Mal;nucleotides 6045-6069 and 6207-6230 of the vpu gene of HIV-1 Eli; andnucleotides 6307-6285 of a nucleic acid sequence complementary to the vpu gene of HIV-1 Eli;b) introducing said amplified nucleotide sequence into a vector;c) transforming a host cell with said vector; andd) placing said transformed host cell in culture and recovering said polypeptide fragment from said culture.