Patent ID: 6521449
Filing Date: 2003-02-18
Classification: A61K,C12N

Abstract:
A gene construct for the expression of a polypeptide, comprising:(a) an enhancer comprising an element or combination of elements, wherein said element or combination of elements is selected from the group consisting of: (i) a combination comprising the following elements obtained from the immunoglobulin &kgr; locus: the &kgr; intron enhancer (&kgr;Ei), the &kgr; 3â€² enhancer (&kgr;E3â€²), and the immunoglobulin &kgr; matrix attachment region (&kgr;MAR); (ii) a combination comprising the following elements obtained from the immunoglobulin heavy chain &mgr; locus: the &mgr;0 intron enhancer (&mgr;Ei) and the &mgr; 3â€² enhancer obtained from a region located 3â€² of C&agr; (&mgr;E3â€²); (iii) a combination of one or more elements selected from the group consisting of &kgr;Ei and &kgr;E3+ and one or more elements selected from the group consisting of &mgr;Ei and &mgr;E3â€²; (iv) the enhancer element of the immunoglobulin &lgr; locus (&lgr;E); (v) a combination of &lgr;E and one or more elements selected from the group consisting of &kgr;Ei and &kgr;E3â€²; and (vi) a combination of &lgr;E and one or more elements selected from the group consisting of &mgr;Ei and &mgr;E3â€²; (b) a promoter operably linked to said enhancer; (c) a nucleotide sequence operably liked to said promoter, said nucleotide sequence encoding a polypeptide selected from the group consisting of: a cytokine, a viral antigen, a cellular adhesion protein, a tumor antigen with the proviso that the c-myc-antigen is excluded, a co-stimulatory signalling molecule, an HLA antigen, and &bgr;-galactosidase; and (d) a polyadenylation site (PAA) operably linked to said nucleotide sequence.