Patent ID: 8574896
Filing Date: 2013-11-05
Classification: C12Q,G01N

Abstract:
1. A reagent useful for a colorimetric method comprising: transferring an electron from an analyte to a coloring reagent that produces color by reduction via a mediator by using an oxidoreductase; and performing qualitative or quantitative analysis of the analyte by measuring color produced in the coloring reagent, and; the reagent comprising: wherein the mediator is at least one selected from the group consisting of a copper complex, an iron complex, an osmium complex, and a ruthenium complex, wherein iron complex has a at least one ligand selected from the group consisting of ammonia, an imidazole compound, a phenanthroline compound, a triazine compound, a biquinoline compound, a pyridylazo compound, a nitroso compound, an oxine compound, a benzothiazole compound, and a derivative of each of the compounds, or the iron complex is selected from the group consisting of Fe(4,4′-dimethyl-2,2′-bipyridyl) wherein the copper complex, the osmium complex, and the ruthenium complex have at least one ligand selected from the group consisting of ammonia, a bipyridyl compound, an imidazole compound, a phenanthroline compound, a triazine compound, a biquinoline compound, a pyridylazo compound, a nitroso compound, an oxine compound, a benzothiazole compound, and a derivative of each of the compounds.