Patent ID: 8178328
Filing Date: 2012-05-15
Classification: C12N,C12P,C12Y,Y02E,Y02P

Abstract:
1. A recombinant microbial host cell comprising an engineered isobutanol biosynthetic pathway, wherein said isobutanol biosynthetic pathway comprises the following substrate to product conversions: (a) pyruvate to acetolactate; (b) the acetolactate from (a) to 2,3-dihydroxy-isovalerate; (c) the 2,3-dihydroxy-isovalerate from (b) to α-ketoisovalerate; (d) the α-ketoisovalerate from (c) to isobutyraldehyde; and (e) the isobutyraldehyde from (d) to isobutanol; wherein i) the substrate to product conversion of step (a) is performed by a recombinantly expressed acetolactate synthase enzyme; ii) the substrate to product conversion of step (b) is performed by a recombinantly expressed acetohydroxy acid isomeroreductase enzyme; iii) the substrate to product conversion of step (c) is performed by a recombinantly expressed acetohydroxy acid dehydratase enzyme; iv) the substrate to product conversion of step (d) is performed by a recombinantly expressed decarboxylase enzyme; and v) the substrate to product conversion of step (e) is performed by an alcohol dehydrogenase enzyme; wherein two or more enzymes of i)-v) are encoded by chimeric genes; and wherein said recombinant microbial host cell is capable of producing isobutanol from pyruvate through the substrate to product conversions of (a) to (e).