Patent ID: 9187788
Filing Date: 2015-11-17
Classification: C12Q,G16B

Abstract:
1. A method for diagnosing acute myeloid leukemia (AML) or a susceptibility to AML in a subject comprising: (a) amplifying a 5′ region of a target gene transcript, if present, in a biological sample with one or more 5′ target primer pairs which are complementary to the 5′ region of the target gene; (b) amplifying a 3′ region of the target gene transcript, if present, in the biological sample with one or more 3′ target primer pairs which are complementary to the 3′ region of the target gene; (c) detecting the amounts of amplification products produced by the one or more 5′ target primer pairs and the one or more 3′ target primer pairs; (d) comparing the relative expression of the 5′ region to the 3′ region of the target gene in the biological sample to the relative expression of the 5′ region to the 3′ region of the target gene in a reference sample to determine if the target gene is dysregulated; and (e) diagnosing the subject as having AML or a susceptibility to AML when the comparison of step (d) indicates that the target gene is dysregulated.