Patent ID: 8546113
Filing Date: 2013-10-01
Classification: C12N,C12P,C12R

Abstract:
1. A method for producing optically active mandelic acid or D-phenylalanine with an isolated NADH oxidase consisting of the amino acid sequence of SEQ ID NO:18 from a microorganism belonging to the genus Brevibacterium comprising the following enzymological features of: (1) catalyzing an NADH oxidization reaction comprising oxygen as a receptor so as to form NAD (2) having an optimum pH of approximately 8 to 10; (3) being not deactivated even under heat treatment at 70° C. for 1 hour and having a residual activity of 80% or more; (4) having an optimum temperature of 50° C. to 70° C.; (5) being activated by an ammonium salt; (6) having a molecular weight of 50 to 60 kpa when subjected to determination by SDS-PAGE, (7) having a low degree of NADPH-oxidizing activity and not being activated by FAD or FMN; and (8) having a Km value of approximately 0.022 mM.