Patent ID: 9012215
Filing Date: 2015-04-21
Classification: C12Q,G01N

Abstract:
1. A method for identifying the presence of leukemic stem cells (LSC) in a sample from a subject suffering from acute myeloid leukemia (AML) comprising: a) obtaining a biological sample from a subject; b) isolating mononuclear cells from the sample of a); c) allowing the cells of b) to take up a fluorescent substrate for the enzyme aldehyde dehydrogenase (ALDH) and labeling the cells with a conjugated anti-CD34 monoclonal antibody and a conjugated anti-CD38 monoclonal antibody and a conjugated anti-CD38 monoclonal antibody; d) isolating CD34 e) measuring the ALDH activity of the cells or population of cells of d); f) providing a reference non-AML sample; g) comparing the levels of ALDH activity in the cells or population of cells from e) with the levels of ALDH activity in the cells or population of cells from the non-AML sample; and h) identifying the cells or population of cells from g) that have an intermediate level of ALDH activity (CD34