Patent ID: 8778347
Filing Date: 2014-07-15
Classification: A61K,C07K,C12Q,G01N

Abstract:
1. A method for determining a viral neutralizing activity of a test antibody, comprising: a) incubating a test antibody with a fluorescently-labeled virus to form a mixture, b) incubating a population of target cells with the mixture of a) under conditions permitting endocytosis of the labeled virus by the target cells, c) incubating the population of target cells of b) with a quencher of fluorescence of the labeled virus bound to the surface of the cells, d) staining the population of target cells of c) with a dye, e) measuring fluorescence of labeled virus endocytozed by the target cells, and f) comparing the fluorescence measured in e) with fluorescence of fluorescently-labeled virus measured in a control experiment where the labeled virus was not incubated with a test antibody, thereby determining a neutralizing activity of a test antibody.