Patent ID: 7544665
Filing Date: 2009-06-09
Classification: A61K,C07K,C12N

Abstract:
1. A method of preparing a peptide having the sequence WQEWEQKITALLEQAQIQQEKNEYELQKLDKWASLWEWF (SEQ ID NO:1) comprising the steps of: (a) providing peptide intermediate fragments WQEWEQKITALLEQAQIQQE (SEQ ID NO:2) and KNEYELQKLDKWASLWEW (SEQ ID NO:3), wherein SEQ ID NO:2 and SEQ ID NO:3 have been synthesized on solid supports utilizing a loading factor of 0.5 or less; (b) in solution phase, reacting KNEYELQKLDKWASLWEW (SEQ ID NO:3) with phenylalaninamide to provide KNEYELQKLDKWASLWEWF (SEQ ID NO:4); and (c) in solution phase, reacting WQEWEQKITALLEQAQIQQE (SEQ ID NO:2) with KNEYELQKLDKWASLWEWF (SEQ ID NO:4) to provide WQEWEQKITALLEQAQIQQEKNEYELQKLDKWASLWEWF (SEQ ID NO:1).