Patent ID: 6261815
Filing Date: 2001-07-17
Classification: C12M,C12N

Abstract:
A method for introducing an exogenous compound into cells, comprising:providing a cell support, with said cell support having a top surface portion with cells adhered thereto, and with said cells being in contact with an electroporation solution;positioning a pair of electrodes in said electroporation solution;positioning a micropipette at a fixed position between and through said pair of electrodes, with said micropipette having an outlet opening in fluid contact with said electroporation solution;introducing a sample having a volume less than 50 microliters and comprising said compound through said outlet opening and into said electroporation solution; andsubjecting said cells to a current pulse across said pair of electrodes, said current pulse sufficient to cause said compound to pass through the cell membranes of said cells and into said cells by electroporation.