Patent ID: 6514697
Filing Date: 2003-02-04
Classification: A61K,C07K,C12N,C12Q,G01N,Y02A

Abstract:
A method for detection of a Cryptosporidium species or isolate in a biological and environmental sample, said method comprising steps:a) obtaining the biological or environmental sample; b) contacting the sample of step (a) with an Cryptosporidium GP900, P68 or cryptopain antigen, DNA or RNA or with a specific anti-Cryptosporidium GP900, P68 or cryptopain antibody, wherein said GP900 antigen is a protein comprising 1832 amino acids depicted by SEQ ID NO: 5 or a protein depicted by any of the SEQ ID NOs: 7-19, or a protein comprising 1721 amino acids depicted by SEQ ID NO: 6 or a protein depicted by SEQ ID NO: 20; wherein said P68 antigen is a protein comprising 503 amino acids depicted by SEQ ID NO: 66 or a protein encoded by the nucleotide sequence depicted by SEQ ID NOs: 64 and 65; wherein said cryptopain is a protein comprising 401 amino acids depicted by SEQ ID NO: 91 or a protein depicted by any of the SEQ ID NOs: 92, 93 or 111-113; wherein said GP900 DNA is a nucleotide sequence comprising 7334 base pairs, 5511 base pairs, 5318 base pairs or 5163 base pairs, depicted by SEQ ID NOs: 1-4 or a nucleotide sequence encoding a protein depicted by any of the SEQ ID NO: 7-20; and wherein said P68 DNA is a nucleotide sequence comprising 2380 base pairs depicted by SEQ ID NO: 65 or 1509 base pairs depicted by SEQ ID NO: 64; and wherein said cryptopain is a nucleotide sequence comprising 1663 base pairs, 534 base pairs, or 678 base pairs, depicted as SEQ ID NOs: 88-90 or a nucleotide sequence depicted by SEQ ID NOs: 94 and 114; and wherein said antibody is monoclonal or polyclonal; and c) detecting a formation of an antigen-antibody complex, or a presence of DNA or RNA; wherein a presence of said antigen-antibody complex, or a presence of DNA or RNA indicates a presence and a positive detection of Cryptosporidium in the sample and an absence of such complex, or absence of DNA or RNA indicates an absence of Cryptosporidium.