Patent ID: 7659103
Filing Date: 2010-02-09
Classification: A61K,A61P,C07K,C12N,G01N

Abstract:
1. A replicon RNA, comprising a nucleotide sequence comprising a 5′ untranslated region comprising the nucleotide sequence of SEQ ID NO: 1, a core protein coding sequence comprising the nucleotide sequence of SEQ ID NO: 2, an E1 protein coding sequence comprising the nucleotide sequence of SEQ ID NO: 3, an E2 protein coding sequence comprising the nucleotide sequence of SEQ ID NO: 4, an NS2 protein coding sequence comprising the nucleotide sequence of SEQ ID NO: 5, an NS3 protein coding sequence comprising the nucleotide sequence of SEQ ID NO: 6, an NS4A protein coding sequence comprising the nucleotide sequence of SEQ ID NO: 7, an NS4B protein coding sequence comprising the nucleotide sequence of SEQ ID NO: 8, an NS5A protein coding sequence comprising the nucleotide sequence of SEQ ID NO: 9, an NS5B protein coding sequence comprising the nucleotide sequence of SEQ ID NO: 10, and a 3′ untranslated region of genomic RNA comprising the nucleotide sequence of SEQ ID NO: 11, at least one selectable marker gene and/or at least one reporter gene, and at least one IRES sequence.