Patent ID: 9080195
Filing Date: 2015-07-14
Classification: C12P,C12Y

Abstract:
1. A reaction system for producing poly (alpha 1,3 glucan), wherein said reaction system comprises: a) a first chamber that comprises an enzyme reaction solution comprising: b) a second chamber, separated from the first chamber by a semi-permeable membrane that is in contact with the enzyme reaction solution, wherein the semi-permeable membrane is permeable to fructose and sucrose, but impermeable to poly (alpha 1,3 glucan) and the glucosyltransferase enzyme, and wherein the semi-permeable membrane facilitates continuous removal of fructose and other low molecular weight moieties from the first chamber while retaining poly (alpha 1,3 glucan) and the at least one glucosyltransferase enzyme inside the first chamber, wherein the semi-permeable membrane has a molecular weight cut-off from 12,000 to 100,000 Daltons.