Patent ID: 8168445
Filing Date: 2012-05-01
Classification: C07K,G01N

Abstract:
1. A method of immobilizing a target molecule on a substrate including: providing said substrate and said target molecule to be immobilized thereon, wherein said target molecule has not been modified to facilitate binding with a metal ion; providing a metal ion having co-ordination sites capable of binding with said substrate and said target molecule; providing a co-ordination ligand in the form of an additive ligand, said co-ordination ligand selected for enhancing or weakening the binding affinity of said metal ion for said substrate or said target molecule; contacting said metal ion and said co-ordination ligand with said target molecule and said substrate, thereby forming a co-ordination complex in which said substrate and said target molecule are bound to co-ordination sites of said metal ion, and in which said metal ion is arranged to link said target molecule with said substrate, thereby immobilizing said target molecule on said substrate, wherein said additive ligand is an amine containing molecule and wherein said metal ion is chromium.