Patent ID: 6489130
Filing Date: 2002-12-03
Classification: A61P,C12N

Abstract:
A method for determining the kinase activity of an isolated polypeptide comprising combining the polypeptide with a substrate and detecting the phosphorylation of the substrate, wherein the polypeptide comprises an amino acid sequence selected from the group consisting of:(a) SEQ ID NO:2; (b) amino acids 1-786 of SEQ ID NO:2; (c) amino acids 17-297 of SEQ ID NO:2; (d) the amino acid sequence of a fragment of a kinase domain of at least 20 amino acids, the amino acid sequence of said kinase domain extending from amino acid 17 to amino acid 297 of SEQ ID NO:2, wherein said fragment has kinase activity; and (e) the amino acid sequence of a polypeptide produced by a method comprising culturing a host cell in a suitable culture medium, wherein the host cell comprises a recombinant vector comprising a nucleic acid encoding said polypeptide and selected from the group consisting of: (e1) an isolated nucleic acid encoding an amino acid sequence comprising the sequence of SEQ ID NO:2; (e2) an isolated nucleic acid encoding amino acids 1-786 of SEQ ID NO:2; (e3) an isolated nucleic acid encoding amino acids 17-297 of SEQ ID NO:2; and (e4) an isolated nucleic acid encoding the amino acid sequence of a fragment of a kinase domain of at least 20 amino acids, the amino acid sequence of said kinase domain extending from amino acid 17 to amino acid 297 of SEQ ID NO:2, wherein the fragment has kinase activity.