Patent ID: 6020148
Filing Date: 2000-02-01
Classification: C07D,C07K,C12Q,G01N,Y10S

Abstract:
A method for testing in vitro epithelial and ocular irritancy of a test material comprising:A. selecting the test material from the group consisting of a neat liquid, a water-insoluble solid, and a water-insoluble gel-like material;B. applying the test material directly to at least one side of a planar non-permeable sample holder;C. contacting the side of the sample holder with the test material applied thereto directly to a cell co-culture whereby the test material is contacted to the cell co-culture for a time period from 10 seconds to 60 minutes; andD. measuring irritancy of the test material by using one or more assays selected from the group consisting of a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, a LDH release assay, and a PGE, release assay.