Patent ID: 6361937
Filing Date: 2002-03-26
Classification: C12Q,G16B

Abstract:
A computer implemented method of sequencing a nucleic acid sequence, the method comprising the steps of:inputting hybridization intensities into a computer for a plurality of nucleic acid probes, the nucleic acid probes hybridizing with the nucleic acid sequence under conditions that do not allow identification of all nucleic acid probes of the plurality of nucleic acid probes that are perfectly complementary to part of the nucleic acid sequence; the computer selecting nucleic acid probes from the plurality of nucleic acid probes, wherein the step of selecting nucleic acid probes includes selecting nucleic acid probes with highest numbers of single base mismatch neighbors among the probes, a single base mismatch neighbor being another probe that has the same sequence except for a single base that is different; and the computer sequencing the nucleic acid sequence according to the selected nucleic acid probes.