Patent ID: 6461807
Filing Date: 2002-10-08
Classification: C12Q

Abstract:
A method of determining that a specific cellular constituent present in a cell type is a target of a drug, said method comprising:(a) identifying cellular constituents as perturbed or as not perturbed in a wild-type cell of said cell type that is exposed to said drug in comparison to a wild-type cell of said cell type that is not exposed to said drug, (b) identifying cellular constituents as perturbed or as not perturbed in a cell of said cell type that both is exposed to said drug and also has said specific cellular constituent modified in comparison to a cell of said cell type that has said specific cellular constituent modified and is not exposed to said drug; (c) identifying cellular constituents that drop out by a method comprising determining each of said cellular constituents that is both identified in step (a) as perturbed and that is also identified in step (b) as either differently perturbed or not perturbed; and (d) ascertaining whether each said cellular constituent identified in step (c) to drop out is also identified as perturbed in a cell of said cell type that has said specific cellular constituent modified in comparison to a wild-type cell of said cell type, whereby said specific cellular constituent is determined as a target of said drug if each said cellular constituent identified to drop out in step (c) out is also ascertained as perturbed in step (d).