Patent ID: 7276382
Filing Date: 2007-10-02
Classification: G01N,Y10T

Abstract:
1. A blood test method comprising the steps of (a) centrifuging a mammalian blood sample into a plasma and blood cells; (b) removing buffy coats from said blood cells to obtain red blood cells containing solutes confined therewithin; (c) washing said red blood cells with a buffered physiological saline solution and isolating said washed blood cells; (d) mixing said washed red blood cells with a buffered physiological saline solution to obtain a suspended liquid; (e) centrifuging said suspended liquid to remove a supernatant and to obtain a red blood cell layer; (f) mixing said red blood cell layer with a hypertonic solution and maintaining the resulting suspension at a temperature of 25 to 40° C. for a period of time sufficient for the solutes confined in the red blood cells to penetrate into the hypertonic solution; (g) centrifuging the suspension to obtain a supernatant containing the solutes; and (h) measuring the supernatant for at least one factor selected from the group consisting of glucose concentration, pyruvic acid concentration, lactic acid concentration and oxidation-reduction potential.