Patent ID: 9222084
Filing Date: 2015-12-29
Classification: C12N

Abstract:
1. A method for separating double-stranded and single-stranded nucleic acids comprising: a) adjusting the salt content of a lysed or homogenized sample containing double-stranded and single-stranded nucleic acids with a non-chaotropic aqueous saline solution having a salt concentration greater than 1M until the double-stranded nucleic acids bind to a first solid carrier, but the single-stranded nucleic acids remain in solution; wherein said adjusting is performed without the addition of all of the following substances: alcohol, EDTA, and EGTA; b) removing the first solid carrier to which the double-stranded nucleic acids are bound; and c) recovering the single-stranded nucleic acid from the adjusted sample solution, wherein recovering the single-stranded nucleic acid from the adjusted sample solution comprises mixing it with an alcoholic solution containing 1 to 90 vol. % alcohol and contacting the resulting mixture with a second solid carrier that binds to single-stranded nucleic acids, wherein the alcoholic solution comprises an alcohol having 1 to 5 carbon atoms, and wherein the alcoholic solution comprises a) a sodium acetate buffer or a potassium acetate buffer, or b) an alcohol-detergent mixture.