Patent ID: 9068993
Filing Date: 2015-06-30
Classification: C07K,G01N

Abstract:
1. A method for detecting presence of antibodies to Wuchereria bancrofti or Brugia malayi infective larvae (L3) in a sample, the method comprising: a) contacting the sample with a first binding reagent which comprises a fusion protein having a reporter domain and a binding domain, the binding domain comprising Wb123 antigen, and forming an immunocomplex, wherein the Wb123 antigen is encoded by the polynucleotide sequence of SEQ ID NO: 1; b) contacting the immunocomplex of a) with an immobilized second binding reagent, the second binding reagent being capable of binding to the antibodies if the antibodies are present in the sample, the first and second binding reagents being capable of simultaneously binding to the antibodies if the antibodies are present in the sample, such that the first binding reagent becomes immobilized through the antibodies bound to the second binding reagent; c) detecting whether the first binding reagent has become immobilized to detect the presence or concentration of the antibodies; and d) correlating the detection of the immobilization of the first binding reagent with the presence of antibodies to