Patent ID: 8535943
Filing Date: 2013-09-17
Classification: C12M,C12N

Abstract:
1. A method for making and isolating platelets comprising: culturing platelet precursor cells in a vessel which contains therein a composite membrane, wherein the composite membrane is a porous support membrane with at least one porous thin membrane provided on at least one side of the porous support membrane, such that the platelet precursor cells are confined within the composite membrane; the porous thin membrane having a porosity of 5 to 80%, an average pore diameter D of 0.5 to 20 μm, and a ratio (σd/D) of a pore diameter standard deviation ad (μm) to the average pore diameter D of 0 to 0.6, and the porous support membrane having an average flow pore size of 1 μm or more; inducing differentiation of the platelet precursor cells into platelets such that the platelets flow through the composite membrane but the platelet precursor cells are maintained within the composite membrane; and collecting the platelets.