Patent ID: 9213029
Filing Date: 2015-12-15
Classification: C07K,G01N

Abstract:
1. A method for detecting breast cancer in a subject, the method comprising the steps of: contacting a sample from blood, nipple aspirate or ductal lavage comprising vesicles from a subject with an anti-PIGR antibody or antibody fragment, wherein the anti-PIGR antibody or fragment thereof binds to vesicles that comprise PIGR to form a complex of the anti-PIGR antibody or fragment thereof, PIGR or fragment thereof, and vesicle; measuring the amount of the complex formed to determine the amount of PIGR or a fragment thereof present in the sample; and comparing the amount of PIGR or fragment thereof in the sample to a control, wherein breast cancer is indicated when the amount of PIGR or a fragment thereof in the sample is higher than that of a negative control, wherein the negative control is the PIGR level of a cancer-free subject measured under the same conditions as used to measure the PIGR level of the sample from the subject; wherein the method further comprises separating the vesicles from the sample before or after contacting the sample with the anti-PIGR antibody or a fragment thereof and before the step of measuring the amount of the complex formed.