Patent ID: 6077691
Filing Date: 2000-06-20
Classification: A61K,C07K,C12N,G01N,Y10S

Abstract:
A method for high yield production of hepatitis B virus preS1-preS2-S large antigen comprising the steps of(a) infecting a mammalian kidney cell witha chimeric vaccinia virus which comprises a synthetic vaccinia early/late promoter or a bacteriophage T7 promoter operatively linked to a polynucleotide that encodes the complete preS1-preS2-S hepatitis B virus large antigen;(b) culturing the infected cell;(c) lysing the infected cell; and(d) harvesting said antigen, wherein at least 1 .mu.g of hepatitis B virus preS1-preS2-S large antigen is produced per 10.sup.6 cells.