Patent ID: 7049151
Filing Date: 2006-05-23
Classification: G01N,Y10S

Abstract:
1. A method for simultaneous detection and/or determination of a plurality of modified proteins in a sample, comprising: a) adding a sulfate or sulfonate detergent, in a concentration of about 1–10 mM and a plurality of first antibodies capable of binding to a specific target protein to said sample to create a mixture and incubating at a temperature of between about 4 and 37 degrees Celsius, and for a time of from about 2 to about 72 hours, wherein the first antibodies are immobilized on solid support material, each first antibody being differentiable from others by a differentiation parameter, whereby the first antibodies bind to respective target proteins present in the sample; b) removing unbound materials from the locus of the first antibodies; c) contacting the materials from step (b) with one or more second antibodies, each of which is specific to a class or subclass of modified proteins or with a plurality of second antibodies, each of which is specific to a modified protein, so as to bind the second antibody or antibodies to modified proteins in the sample; and d) detecting and/or determining a plurality of modified proteins in the sample.