Patent ID: 8612161
Filing Date: 2013-12-17
Classification: C12Q,G16B

Abstract:
1. A method for determining an identity of a nucleic acid at an interrogation position in a nucleotide sequence from data acquired from one or more channels in parallel, comprising: a) sequencing a nucleic acid by DNA sequencing by synthesis utilizing nucleotides with fluorescent dyes which are incorporated into a complementary strand, b) obtaining a data set for one or more dye intensities at one or more nucleic acid positions in said complementary sequence, wherein each dye corresponds to a nucleotide, c) determining the ratio contribution to dye intensity at said interrogation position from dye intensities at the interrogation position and at one or both of d) applying said ratio contribution to said data set to arrive at an identity for a nucleotide at said interrogation position in said nucleotide sequence.