Patent ID: 6140087
Filing Date: 2000-10-31
Classification: A01K,C12N

Abstract:
A kit for making an adenovirus vector comprising nucleic acid sequences of a first plasmid and a second plasmid, said kit comprising a first and a second plasmid:(a) wherein said first plasmid comprises:(i) a modified adenovirus genome, wherein said genome comprises a modification within early region 1 (E1) that comprises a deletion of a packaging signal of the early region 1 which renders said first plasmid incapable of forming viable viral particles in host cells, by eliminating susceptibility of adenoviral sequences encoded by said first plasmid to being encapsidated into a viral particle, but does not affect the ability of the adenovirus genome to replicate, and(ii) at least one nucleic acid sequence for (A) encoding antibiotic resistance and (B) replication of said first plasmid in host cells; and(b) wherein said second plasmid is an E1 shuttle plasmid, which comprises at least one nucleic acid sequence comprising adenovirus genome E1 region sufficient to rescue said first plasmid via recombination of said first plasmid and said second plasmid to produce said adenovirus vector.