Patent ID: 6201109
Filing Date: 2001-03-13
Classification: C07K

Abstract:
A method for determining the presence of bone alkaline phosphatase in a sample suspected of containing said bone alkaline phosphatase, which comprises:(a) bringing together in an aqueous medium:(1) said sample,(2) a first antibody capable of specifically binding to a first epitopic site on said bone alkaline phosphatase,(3) a second antibody capable of specifically binding to a second epitopic site on said bone alkaline phosphatase,wherein said first and second epitopic sites are different and said first and second antibodies together form an immunocomplex with said bone alkaline phosphatase, if present, and wherein said first and second antibodies are selected from the group consisting of a monoclonal antibody produced by hybridoma cell line ATCC No. HB 11106, a monoclonal antibody produced by hybridoma cell line ATCC No. HB 11107, and a monoclonal antibody produced by hybridoma cell line ATCC No. HB 11108, with the proviso that said first and second antibodies are from different cell lines; and(b) examining said medium for the presence of said immunocomplex, the presence thereof being related to the presence of bone alkaline phosphatase in said medium.