Patent ID: 8936925
Filing Date: 2015-01-20
Classification: C12N

Abstract:
1. A method for reconstitution of an active 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) structure from at least two inactive EPSPS fragments which constitute an EPSPS fragment pair, said method comprising, providing a first inactive EPSPS fragment and a second inactive EPSPS fragment, allowing the first and the second fragments to associate to form a structure characterized in that it, exhibits EPSPS activity; lacks a covalent bond joining the fragments; and lacks complementary non-EPSPS sequences, the fragment pair being selected from a group of E. coli EPSPS fragment pairs consisting of the following: N67/C68, N85/C86, N104/C105, N154/C155, N182/C183, N184/C185, N218/C219, N224/C225, N227/C228, N259/C260, N298/C299, N371/C372, N376/C377, N383/C384, or is selected from a group of P. putida CGMCC 0739 EPSPS fragment pairs consisting of the following: N208/C209, N214/C215, N219/C220, N222/C223, N224/C225.