Patent ID: 8409803
Filing Date: 2013-04-02
Classification: C12N,C12Q,G01N

Abstract:
1. A method for identifying, detecting or quantifying the presence of at least one target Respiratory Syncytial Virus (RSV) nucleic acid in a sample, comprising the following steps: (a) providing two or more oligonucleotide components having the ability to anneal to the target RSV nucleic acid, wherein a first oligonucleotide component and a second oligonucleotide component are capable of forming a catalytically active multi-component nucleic acid enzyme (MNAzyme) once annealed to the target RSV nucleic acid; (b) contacting said two or more oligonucleotide components with said sample wherein said sample permits the binding of said at least one target RSV nucleic acid to said two or more oligonucleotide components and formation of the MNAzyme; (c) contacting the MNAzyme with a catalyzable nucleic acid reporter substrate that is separate from the at least one target RSV nucleic acid; and (d) identifying, detecting or quantifying the presence of the target RSV nucleic acid by detecting catalysis of the nucleic acid reporter substrate.