Patent ID: 9128094
Filing Date: 2015-09-08
Classification: G01N

Abstract:
1. A method for quantifying an HPV protein expressed by a human papillomavirus (HPV) infected cell in a clinical sample, comprising the steps of: receiving a clinical sample, said clinical sample comprising a population of cells susceptible to infection by an HPV protein, wherein depositing said clinical sample onto a substrate without lysing said population of cells; contacting said clinical sample with a solution comprising a monoclonal antibody that specifically binds to the HPV protein in situ under conditions that promote specific binding of said monoclonal antibody to the HPV protein expressed by one or more cells in said population of cells from said obtained clinical sample, wherein determining, in a solution phase, the amount of said monoclonal antibody bound to said clinical sample; and quantifying the HPV protein in said clinical sample using the amount of said monoclonal antibody bound to said clinical sample.