Patent ID: 8492113
Filing Date: 2013-07-23
Classification: G01N

Abstract:
1. A method for measuring recombinant protein concentration in bacterial inclusion body (IB) harvests comprising the steps of i) measuring the total weight of dry solids from an aliquot of an inclusion body harvest slurry, ii) multiplying total weight of dry solids in the aliquot of an IB harvest slurry using a pre-determined protein productivity conversion factor (PPCF) in a formula, wherein the product of the formula provides total recombinant protein concentration in said aliquot of said IB harvest slurry, and wherein the PPCF for said recombinant protein is calculated in an aliquot of an IB harvest slurry by multiplying the ratio of recombinant protein in said aliquot to total dry solids in said aliquot by 1000, wherein the PPCF for one inclusion body harvest aliquot is used to calculate recombinant protein concentration from different fermentation inclusion body harvests carried out following the same protocol, iii) measuring the total IB harvest slurry weight and the total weight from the aliquot of IB harvest slurry, and iv) multiplying the product of (ii) by the ratio of total IB harvest slurry weight to the total weight from the aliquot of IB harvest slurry, wherein the product of iv) provides the total recombinant protein concentration in the bacterial inclusion body (IB) harvest.