Patent ID: 7585625
Filing Date: 2009-09-08
Classification: C12Q

Abstract:
1. A process for preparing at least one sample for a method of detecting and quantifying a total amount of HIV nucleic acid present in the at least one sample, said process comprising: a) administering at least 100 microliters of the at least one sample to a piece of filter paper capable of absorbing the at least one sample, wherein the absorption results in at least one spot comprising at least 100 μL of the at least one sample on the filter paper, b) drying the filter paper having the absorbed at least one spot comprising at least 100 μL of the at least one sample, c) storing the filter paper for at least one week, d) excising the at least one spot comprising at least 100 μL of the at least one sample from the surrounding filter paper, e) extracting nucleic acid from all of the at least one spot comprising at least 100 μL of the at least one sample with a chaotropic nucleic acid isolation solution, f) detecting HIV nucleic acid, if present, and g) quantifying the total amount of the HIV nucleic acid present in the at least one sample.