Patent ID: 7022491
Filing Date: 2006-04-04
Classification: G01N,Y10S,Y10T

Abstract:
1. A method for identifying an interacting target biomolecule to a biomolecule of interest comprising the steps of: (a) providing a biomolecule of interest having specificity for the target; (b) binding the biomolecule of interest to at least one type of linker molecule, the linker molecule including at least one attachment part for binding to the biomolecule of interest, one cleavable part, one mass marker part and one photoactivatable part, for binding to the target; (c) contacting the biomolecule of interest with a cell or a cell extract; (d) exposing the cell to photolysis, whereby the photoactivatable part binds to the target; (e) cleaving the linker molecule or molecules, thereby leaving the photoactivatable part and the mass marker part bound to the target; and (f) analysing the product of step (e), thereby detecting the mass marker part, thus identifying the interacting target biomolecule to the biomolecule of interest.