Patent ID: 8663913
Filing Date: 2014-03-04
Classification: A61K,C07K,C12N,C40B

Abstract:
1. A method of preparing a lambda phage particle comprising: providing a permissive host bacterial cell comprising an expression system comprising a nucleic acid molecule comprising lambda pR promoter consisting of the nucleotide sequence as set forth in SEQ ID NO:2 and operably linked to a nucleic acid molecule encoding a Shine-Delgarno sequence consisting of the nucleotide sequence as set forth in SEQ ID NO:4 and operably linked to a nucleic acid molecule encoding lambdaphage D gene consisting of the nucleotide sequence as set forth in SEQ ID NO:1, said lambdaphage D gene sequence comprising inserted in frame with the lambdaphage D gene a nucleic acid molecule encoding a gene of interest, said permissive host bacterial cell being suitable for vegetative growth of lambdaphage and further comprising the cl subjecting the host bacterial cell to conditions such that bacteriophage particle expression and assembly is initiated; and recovering the bacteriophage particles from the culture, thereby providing a lambda phage particle.