Patent ID: 7211382
Filing Date: 2007-05-01
Classification: C12Q

Abstract:
1. A method for inhibiting template independent primer extension, comprising: employing a primer extension reaction using i) a polymerase, ii) a template, iii) a plurality of primers having a palindromic nucleotide sequence, by: hybridizing a first primer of the plurality of primers to the template so as to form a primer-template duplex; hybridizing a second of the plurality of primers to a third of the plurality of primers so as to form a primer-primer duplex, and each of the plurality of primers comprise one or more modified nucleotides at or near the 3′ or 5′ end of the palindromic nucleotide sequence that inhibit(s) template independent primer extension of the primer-primer duplex, wherein the polymerase extends the primer of the primer-template duplex and the one or more modified nucleotides of the plurality of primers is modified at the 2′ position and the first, second, and third of the plurality of primers have the same nucleotide sequence.