Patent ID: 6344600
Filing Date: 2002-02-05
Classification: A61K,C07K,C12N

Abstract:
A method for producing human hemoglobin protein which reversibly binds oxygen comprising the following steps:i) transforming into plant cells one or more nucleic acid molecule(s) comprising sequences encoding the &agr; and &bgr; polypeptide chains of human hemoglobin; ii) propagating the transformed plant cells in culture or regenerating transgenic or chimeric plants from said transformed plant cells; and iii) recovering and purifying said human hemoglobin protein which reversibly binds oxygen, said recovering due to at least one association in the transformed plant cells of at least one polypeptide chain of &agr; and &bgr; human hemoglobin encoded by said nucleic acid molecules with at least one iron-containing porphyrin nucleus.