Patent ID: 8618293
Filing Date: 2013-12-31
Classification: C07F,C12Q

Abstract:
1. A method for measuring an amount or concentration of an analyte, comprising: (a) contacting a sample which contains the analyte with a solution containing an enzyme that acts on the analyte and a redox mediator of Formula I wherein M is ruthenium or osmium and has an oxidation state of 0, 1, 2, 3 or 4; each of w, x, and y is an integer independently selected from the integers 1 to 4; m is an integer selected from the integers −5 to +4; n is an integer selected from selected from the integers 1 to 5; z is an integer selected from the integers −2 to +1; A is a monodentate 5- or 6-membered aromatic ligand containing 1, 2 or 3 nitrogen atoms which is optionally substituted by 1 to 8 substituents each selected from the group consisting of substituted or unsubstituted alkyl, alkenyl, or aryl groups, —F, —Cl, —Br, —I, —NO B is a bi-, tri-, tetra-, penta- or hexadentate ligand which is linear having the formula R C is a ligand other than B; and X is a counter ion, wherein the number of coordinating atoms is 6, with the exception of [Ru (b) incubating the contacted sample under conditions that cause the enzyme to act on the analyte; (c) subjecting the incubated sample of step (b) to conditions which result in a change in a measurable signal; and (d) measuring the measurable signal to determine the amount or concentration of the analyte in the sample.