Patent ID: 7223571
Filing Date: 2007-05-29
Classification: A61K,A61L,C07H,C07K,C08B,C12N,C12P,C12Q

Abstract:
1. A method for enzymatically producing defined glycosaminoglycan polymers comprising the steps of: providing at least one functional acceptor, wherein the functional acceptor has at least two sugar units selected from the group consisting of uronic acid and hexosamine; providing at least one recombinant glycosaminoglycan transferase having an empty acceptor site and being capable of elongating the at least one functional acceptor in a controlled fashion to form extended glycosaminoglycan molecules, the at least one recombinant glycosaminoglycan transferase selected from the group consisting of: providing at least one UDP-sugar selected from the group consisting of UDP-GlcUA, UDP-GlcNAc, and UDP-GlcN in a stoichiometric ratio to the at least one functional acceptor such that the at least one recombinant glycosaminoglycan transferase elongates the at least one functional acceptor to provide glycosaminoglycan polymers wherein the glycosaminoglycan polymers have a desired size distribution such that the glycosaminoglycan polymers are substantially monodisperse in size such that the glycosaminoglycan polymers have a polydispersity value in a range of from 1.0 to 1.2, and wherein the desired size distribution is obtained by controlling the stoichiometric ratio of UDP-sugar to functional acceptor.