Patent ID: 7323305
Filing Date: 2008-01-29
Classification: B01L,C07H,C12N,C12Q,G01N,G01R,Y02P,Y10T

Abstract:
1. A method for sequencing nucleic acids comprising: (a) fragmenting large template nucleic acid molecules to generate a plurality of fragmented nucleic acids; (b) delivering the fragmented nucleic acids into aqueous microreactors in a water-in-oil emulsion such that a plurality of aqueous microreactors comprise a single copy of a fragmented nucleic acid, a single bead capable of binding to the fragmented nucleic acid, and amplification reaction solution containing reagents necessary to perform nucleic acid amplification; (c) amplifying the fragmented nucleic acids in the microreactors to form amplified copies of said nucleic acids and binding the amplified copies to beads in the microreactors; (d) delivering the beads to an array of at least 10,000 reaction chambers on a planar surface, wherein a plurality of the reaction chambers comprise no more than a single nucleic acid bound bead; and (e) performing a sequencing reaction simultaneously on a plurality of the reaction chambers.