Patent ID: 7208093
Filing Date: 2007-04-24
Classification: B01D,B01J

Abstract:
1. A method of producing a chromatographic separation matrix of porous polymeric particles including two layers with different properties said method comprising: (a) providing at least one porous polymeric particle that presents reactive groups on its pore surfaces and on its external surface; (b) washing said particle with a first solvent and draining the solvent to obtain a first phase enclosed in said at least one particle; (c) wetting an enclosing outer layer of the particle by adding a second solvent, which solvent is essentially insoluble in the first solvent, to provide a second phase on the outer layer; (d) reacting the reactive groups in the outer layer by adding a reagent, which is essentially non-reactive in the first solvent; and (e) coupling chromatographic binding groups to the reactive groups in the inner layer.