Patent ID: 6808905
Filing Date: 2004-10-26
Classification: A61K,C07K,C12N,C12Y

Abstract:
A lentiviral producer cell comprising:(a) a first nucleotide sequence comprising a gag, a pol, or gag and pol genes; (b) a second nucleotide sequence comprising a heterologous env gene; and (c) a third nucleotide sequence comprising a lentiviral transfer vector that comprises a transgene that encodes a blood clotting factor operably linked to an expression control sequence; wherein the producer cell lacks a functional tat gene; and wherein the expression control sequence comprises: (i) an Enh1 enhancer (SEQ ID NO: 10); or (ii) an &agr;-1-microglobulin/bikunin enhancer and a human factor VIII endogenous promoter (L-F8).