Patent ID: 6551788
Filing Date: 2003-04-22
Classification: G01N,Y10S

Abstract:
A method for assaying an analyte in a sample, comprising:a) providing a set of coated particles comprising at least i) a first class of particles having a first analyte-specific binding partner immobilized thereon, and ii) a second class of particles having a second analyte-specific binding partner immobilized thereon, wherein said first and second classes of coated particles are independently determinable, wherein said particles of said first class of particles are smaller than the particles of said second class, and wherein the first binding partner and the second binding partner are the same; b) simultaneously incubating said sample in the presence of said set of particles under conditions that allow the formation of a first complex comprising said analyte bound to said first binding partner immobilized on said first class of particles and/or a second complex comprising said analyte bound to said second binding partner immobilized on said second class of particles; c) correlating the amount of said first complex and/or said second complex to the concentration of said analyte in said sample; and d) determining the concentration of said analyte.