Patent ID: 6432639
Filing Date: 2002-08-13
Classification: C12N,C12Q

Abstract:
An isolated nucleic acid molecule, wherein said isolated nucleic acid molecule specifically hybridizes to and detects a CYP3A4 variant gene that differs from the CYP3A4 wild-type gene by having one or more polymorphisms selected from the group consisting of:(a) a substitution of a G nucleotide for an A nucleotide at position âˆ’392 of the promoter of said CYP3A4 gene with respect to the start codon of said CYP3A4 gene; (b) an insertion of nucleotides 10-51 of SEQ ID NO:46 at position +506 of intron 3 of said CYP3A4 gene with respect to the first nucleotide of said intron 3; (c) a substitution of a C nucleotide for a G nucleotide at position âˆ’9 of intron 4 of said CYP3A4 gene with respect to the first nucleotide of exon 5; (d) a substitution of a G nucleotide for a T nucleotide at position +52 of intron 6 of said CYP3A4 gene with respect to the first nucleotide of said intron 6; (e) a substitution of a T nucleotide for a C nucleotide at position 579 with respect to the first nucleotide of the coding sequence of said CYP3A4 gene; (f) a substitution of a G nucleotide for a T nucleotide at position +34 of intron 7 of said CYP3A4 gene with respect to the first nucleotide of said intron 7; (g) a substitution of an A nucleotide for a G nucleotide at position +12 of intron 10 of said CYP3A4 gene with respect to the first nucleotide of said intron 10; and, (h) a substitution of a T nucleotide for a C nucleotide at position âˆ’11 of intron 11 of said CYP3A4 gene with respect to the first nucleotide of exon 12; and wherein said isolated nucleic acid molecule is selected from the group consisting of: (i) an isolated nucleic acid molecule selected from the group consisting of: an isolated nucleic acid molecule consisting essentially of SEQ ID NO:44, an isolated nucleic acid molecule consisting essentially of SEQ ID NO:46, an isolated nucleic acid molecule consisting essentially of 18-19 contiguous nucleotides of SEQ ID NO:48, an isolated nucleic acid molecule consisting essentially of 18-19 contiguous nucleotides of SEQ ID NO:50, an isolated nucleic acid molecule consisting essentially of 15-19 contiguous nucleotides of SEQ ID NO:52, an isolated nucleic acid molecule consisting essentially of 18-19 contiguous nucleotides of SEQ ID NO:54, an isolated nucleic acid molecule consisting essentially of 18-19 contiguous nucleotides of SEQ ID NO:56, and an isolated nucleic acid molecule consisting essentially of 15-18 contiguous nucleotides of SEQ ID NO:58; (ii) a nucleic acid probe consisting essentially of about 100 or fewer nucleotides, wherein said probe comprises said nucleic acid molecule of (i); (iii) a recombinant nucleic acid molecule consisting essentially of said nucleic acid molecule of (i) and a vector sequence; (iv) an isolated CYP3A4 variant gene that differs from a wild-type CYP3A4 gene only by having one or more polymorphisms selected from the group consisting of (a)-(h), wherein said isolated CYP3A4 gene comprises said nucleic acid molecule of (i); (v) an at least 18 nucleotide fragment of the isolated CYP3A4 variant gene of (iv), wherein said fragment comprises said nucleic acid molecule of (i); and, (vi) a nucleic acid molecule that is fully complementary to a nucleic acid molecule of (i), (ii), (iii), (iv) or (v).