Patent ID: 6366860
Filing Date: 2002-04-02
Classification: C07K,C12N

Abstract:
A method of making a synthetic nucleic acid sequence, the method comprising:(a) providing a starting nucleic acid sequence; (b) determining the predicted &Dgr;Gfolding of the starting nucleic acid sequence; (c) modifying the starting nucleic acid sequence by replacing at least one codon from the starting nucleic acid sequence with a different corresponding codon to provide a modified nucleic acid sequence; (d) determining the predicted &Dgr;Gfolding of the modified nucleic acid sequence; (e) comparing the &Dgr;Gfolding of the modified nucleic acid sequence with the &Dgr;Gfolding of the starting nucleic acid sequence (f) determining whether the &Dgr;Gfolding of the modified nucleic acid sequence is increased relative to the &Dgr;Gfolding of the starting nucleic acid sequence by a desired amount; (g) if the &Dgr;Gfolding of the modified nucleic acid sequence is not increased by the desired amount, further modifying the modified nucleic acid sequence by replacing at least one codon from the modified nucleic acid sequence with a different corresponding codon to provide a different modified nucleic acid sequence; and repeating steps(f) and (g) until the &Dgr;Gfolding of the modified nucleic acid sequence is increased by the desired amount to ultimately provide a final nucleic acid sequence.