Patent ID: 7220539
Filing Date: 2007-05-22
Classification: C12N,C12Q,G01N

Abstract:
1. A method for screening test compounds to determine if any disrupt the interaction between an inhibitor of protein kinase B (PKB)/Akt protein and a PKB/Akt protein, said method comprising: assaying for complex formation between said inhibitor and said PKB/Akt protein in the presence and absence of a test compound, wherein said inhibitor of PKB/Akt protein is a Tribbles (TRB) family protein selected from the group consisting of human TRB-3 having the sequence set forth in SEQ ID NO:1, mouse TRB-3 having the sequence set forth in SEQ ID NO:2, rat TRB-3 having the sequence set forth in SEQ ID NO:3, C5FW having the sequence set forth in SEQ ID NO:4, C8FW having the sequence set forth in SEQ ID NO:5, wherein a decrease in the level of complex formation in the presence of said test compound, relative to complex formation in the absence of said test compound, is indicative of a compound that disrupts said interaction.