Patent ID: 8637272
Filing Date: 2014-01-28
Classification: C12N,C12P

Abstract:
1. An isolated halohydrin epoxidase consisting of an amino acid sequence in which any one of or a plurality of amino acid mutations selected from (A) to (D) below are introduced into an amino acid sequence of a wild-type halohydrin epoxidase having the amino acid sequence represented by SEQ ID NO:2: (A) an amino acid mutation in which Ala at the 2nd position is substituted with Lys or Asn; (B) an amino acid mutation in which Thr at the 133rd position is substituted with Ala, Cys, or Ser; (C) an amino acid mutation in which Phe at the 136th position is substituted with Ala, Ser, or Trp; and (D) an amino acid mutation in which Asp at the 199th position is substituted with Gln, Glu, His, Ser, Thr, Tyr, Leu, Ile, or Met.