Patent ID: 6646243
Filing Date: 2003-11-11
Classification: B01J,B01L,B82Y,C07B,C07C,C07D,C07H,C07K,C08G,C12Q,C40B,G01N,G03F,G11C,H01L,Y02P,Y10S,Y10T

Abstract:
A polynucleotide analysis apparatus comprising:a capillary substrate having a surface that is made of a material that is different from the remainder of the capillary, said surface comprising a plurality of different polynucleotides, each of said different polynucleotides occupying a different area of said surface, each of said areas being 10âˆ’1 cm2 or less in detectable area, said surface being within the capillary and comprising more than 10 of such polynucleotides, at least some of said polynucleotides bearing fluorescently labeled target molecules excitable with a laser; an argon laser light source capable of generating light at a wavelength of about 488 nm or less at said surface to excite said fluorescently labeled target molecules, which generate light at a frequency greater than 488 nm; said laser oriented to direct the laser light at said surface at an angle capable of contacting said target molecules; a CCD detector oriented in a manner capable of detecting light fluoresced from said target molecules in said areas; and a data collection, recording and management system for storing fluoresced light intensity, said data collection, recording and management system coupled to said CCD detector, said data collection, recording and management system storing light intensity and converting said light intensity into nucleic acid sequence information.