Patent ID: 8309321
Filing Date: 2012-11-13
Classification: C12Q

Abstract:
1. A method for identifying a protease secretion deficient strain of a microorganism not deficient in desired protein target secretion comprising the steps of producing a mutant of a wild type microorganism, followed by adding the mutant to gel-filled wells of a microtitration plate, incubating the mutant in the gel-filled wells under conditions causing the mutant to secrete proteins, separating the mutant from the gel, and measuring activity of a secretion protease of the microorganism in the gel, wherein either (i) the gel is a substrate for the secretion protease or (ii) the gel contains a substrate for the secretion protease, a measurement of reduced protease activity compared to the wild type identifying the mutant as a protein deficient strain, and measuring desired protein target secretion in the gel and comparing the measured secretion with desired protein target secretion of the wild type, to confirm whether the mutant is not deficient in desired protein target secretion.