Patent ID: 9074172
Filing Date: 2015-07-07
Classification: C12M

Abstract:
1. A culture plate,comprising: (a) an upper portion, wherein the upper portion further includes at least one discrete walled chamber, having a top edge and a bottom edge; wherein the bottom edge of the at least one discrete chamber includes an energy directing structure formed integrally therewith, wherein the energy directing structure is in the shape of an inverted triangle in cross-section, and wherein the angle of the apex of the inverted triangle is about 60° to about 90°; (b) a substantially flat lower portion, wherein the lower portion further includes: (c) an ultrasonically-created hermetic seal formed between the bottom edge of the at least one discrete chamber and the fiber-coated upper surface of the lower portion, wherein the hermetic seal is formed by applying ultrasonic energy to the lower portion to create a permanent airtight and watertight bond between bottom edge of the at least one discrete chamber and the fiber-coated upper surface of the lower portion of the culture plate, and wherein the hermetic seal formed between the bottom edge of the at least one discrete chamber and the fiber-coated upper surface of the lower portion forms a well for containing cell culture medium.