Patent ID: 6150102
Filing Date: 2000-11-21
Classification: B01J,B82Y,C12N,C12P,C12Q,C40B

Abstract:
A method for providing a set of oligomers comprising known subunit sequences comprising:a) obtaining a device for storing and providing oligomers comprising a substrate that supports an array of oligomer depots;wherein each depot comprises a delimited area or volume at which is attached a plurality of oligomers having a known subunit sequence, said oligomers being oligonucleotides and/or oligonucleotide analogs;wherein the subunit sequence of the oligomers attached to at least one of said depots is different from the subunit sequence of the oligomers attached to a different depot of said array; andwherein oligomers comprising known subunit sequences are stored at a plurality of depots of said array by being hybridized by Watson-Crick pairing to the oligomers attached at said depots to form double-stranded complexes, said stored oligomers also being oligonucleotides and/or oligonucleotide analogs;b) locally heating one or more selected depots of the intact array to denature double-stranded complexes of said selected depots and release oligomers stored therein, without effecting significant denaturation of double-stranded complexes of the unselected depots of the array; andc) collecting the oligomers released as a result of locally heating the selected depots.