Patent ID: 7276590
Filing Date: 2007-10-02
Classification: C07K,C12N

Abstract:
1. A method for purifying a peptide from a mixture comprising said peptide and related impurities, said method comprising: a) eluting said related impurities of said mixture from an anion exchange chromatography matrix using a solution comprising an organic modifier, water, a buffer, and optionally a salt component at a linear or step gradient or isocratically in salt component, and at pH-values maintained with a buffer so that said peptide has a negative local or overall net charge and said related impurities have a local or overall negative net charge which is lower than the negative net charge of said peptide so as to remove said related impurities; and without an intervening step, b) subsequently, eluting said peptide in the absence of an organic modifier, by a step or linear change to an aqueous solvent optionally with a salt component, at the same or lower pH-values maintained with a buffer.