Patent ID: 8017362
Filing Date: 2011-09-13
Classification: C12N,C12P

Abstract:
1. A method for producing L-methionine which comprises culturing an Escherichia coli in a medium to produce and accumulate L-methionine in the medium, and collecting the L-methionine from the medium in an amount in excess of the corresponding unmodified Escherichia bacterium, wherein the Escherichia coli is deficient in a repressor of L-methionine biosynthesis system and has L-methionine productivity, wherein the repressor of L-methionine biosynthesis is the metJ protein, and wherein the Escherichia coli further comprises at least one characteristic selected from the group consisting of: (a) exhibits reduced intracellular S-adenosylmethionine synthetase activity; (b) exhibits L-threonine auxotrophy; (s) exhibits enhanced intracellular cystathionine γ-synthase activity and enhanced intracellular aspartokinase homoserine dehydrogenase II activity; and (d) has a homoserine transsuccinylase for which concerted inhibition by L-methionine and S-adenosylmethionine is desensitized.