Patent ID: 6218149
Filing Date: 2001-04-17
Classification: A61K,C07K

Abstract:
A method of altering affinity of an antibody for an antigen to which it is directed, wherein the method comprises:(a) measuring the affinity of an unaltered antibody for the antigen,(b) deleting DNA encoding a carbohydrate recognition site from DNA encoding the variable region of the antibody thereby producing a mutagenized gene encoding an altered antibody;(c) expressing the mutagenized gene encoding the altered antibody to produce an altered antibody;(d) measuring the affinity of the altered antibody for the antigen; and(e) determining that the affinity measured in step (a) is different from the affinity measured in step (d),wherein the carbohydrate recognition site comprises Asn-X-Thr/Ser, wherein X indicates any amino acid and wherein Thr/Ser indicates either threonine or serine.