Patent ID: 8859235
Filing Date: 2014-10-14
Classification: A61P,C12N,C12P

Abstract:
1. A process for manufacturing a product in a cell culture system comprising bacterial cells, the process comprising: adding at least one first additive selected from the group consisting of a sterically hindered nitroxyl, a sterically hindered hydroxylamine, a sterically hindered hydroxylamine salt compound, a sterically hindered amino compound, and a sterically hindered N-hydrocarbyloxyamine, -to a medium comprising a cell culture to produce a product from the cell in the cell culture, wherein the at least one first additive is added in an amount of 0.0001 to 10% by weight, based on a total weight of the culture; and optionally adding at least one second additive selected from the group consisting of selected from the group consisting of a benzofuranone compound, a nitrone, a phenol antioxidant, a natural antioxidant, a radical scavenger, and a coumestane to the medium, wherein a part or all of the process is carried out in the presence of the first additive or the first and second additives, and each additive may be present in free form, salt form, or both, wherein (i) the sterically hindered nitroxyl compound, sterically hindered hydroxylamine compound, sterically hindered hydroxylamine salt compound is of formulae IA, IB or IC the sterically hindered nitroxyl compound is an imidazoline nitroxide of the formula ID, IE, IF or IG, wherein a compound of the formula IA, ID, IE, IF or IG instead of the N—O (nitroxyl) moiety comprises a moiety N-Rk, wherein Rk is C (ii) the sterically hindered amino compound is of the formula IA*, ID*, IE*, IF* or IG*, wherein X is CH A A Rz is chloro or —N(2-ethylhexyl) Z h is the number of positive charges and j is the number of negative charges, X is an inorganic or organic anion, where the total charge of cations h is equal to the total charge of anions j; D D D D