Patent ID: 6043340
Filing Date: 2000-03-28
Classification: A61K,C07K,C12N,C12Q,C12Y,G01N,Y10T

Abstract:
A synthetic peptide having an amino acid sequence corresponding to from 6 amino acids to all the amino acid sequence encoded by a genetic suppressor element produced by a method of isolating genetic suppressor elements derived from a kinesin gene that confer upon a cell resistance to one or more DNA damaging agents, wherein the method comprises the steps of:(a) generating a set of random subfragments of cDNA encoding the kinesin gene;(b) transferring the DNA fragments to an expression vector to yield a library, wherein each of the DNA fragments is operatively linked to a protein translation initiation codon, and wherein the expression vector expresses the DNA fragments in a living eukaryotic cell;(c) genetically modifying living eukaryotic cells by introducing the random fragment library of step (b) into the living eukaryotic cells;(d) isolating or enriching for genetically modified living eukaryotic cells containing kinesin-derived genetic suppressor elements by selecting cells in the presence of a DNA damaging agent; and(e) obtaining the genetic suppressor element from the genetically modified eukaryotic cells that survive step (d).