Patent ID: 7510881
Filing Date: 2009-03-31
Classification: G01N,Y10S

Abstract:
1. A method for quantitatively and/or qualitatively detecting one or more components in one or more samples, said component capable of binding to a probe, comprising the steps in the following order: a) applying onto a solid support b) incubating the solid support of step a) with: c) incubating the solid support with a monoclonal or polyclonal antibody directed against the tag of step b), said antibody raised in species A and said antibody is labeled with metal particles of average diameter between 0.6 nm and 40 nm, followed by a wash step, d) incubating the solid support with an antibody conjugate, said conjugate comprising: e) incubating the solid support with a polypeptide capable of recognition by anti-B antibodies, said polypeptide labeled with one or more substances which directly or indirectly cause a quantitative color change compared with the solid support, f) incubating the solid support with a metal enhancement reagent, and g) reading the solid support to quantitatively and/or qualitatively detect said components.