Patent ID: 8679740
Filing Date: 2014-03-25
Classification: C12Q

Abstract:
1. A method of amplifying a hepatitis C virus (HCV) nucleic acid from an HCV infected sample, comprising amplifying a segment of a DNA template complementary to a genome of HCV RNA from the sample by a two-stage polymerase chain reaction (PCR), wherein a first stage PCR employs a first outer primer and a second outer primer, and a second stage PCR employs a first inner primer and a second inner primer, wherein: i) the nucleotide sequence of the first outer primer consists of the nucleotide sequence as set forth in SEQ ID NO: 9: ii) the nucleotide sequence of the second outer primer consists of the nucleotide sequence as set forth in SEQ ID NO: 10 or SEQ ID NO: 11: wherein Y is C, T or U; and V is A, C or G; iii) the nucleotide sequence of the first inner primer consists of the nucleotide sequence as set forth in SEQ ID NO: 12 or SEQ ID NO: 15: iv) the nucleotide sequence of the second inner primer consists of the nucleotide sequence as set forth in SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 16, or SEQ ID NO: 17: wherein R is A or G; wherein the amplified HCV nucleic acid has greater than 8,000 base pairs.