Patent ID: 6566083
Filing Date: 2003-05-20
Classification: C07K,C12N,G01N

Abstract:
A method of detecting a biologically active substance affecting intracellular processes mediated through an enzyme or a second messenger, the method comprising:(a) culturing a cell containing a DNA sequence, wherein the DNA sequence encodes a Green Fluorescent Protein (GFP) comprising an enzyme recognition site or comprising a binding domain of a second messenger under conditions permitting expression of the DNA sequence; (b) measuring the fluorescence of said GFP in the cell in the absence and in the presence of a test sample; and (c) comparing the GFP fluorescence measured in step (b); wherein a difference between the fluorescence measured in the absence and in the presence of a test sample indicates the presence in said sample of biologically active substances that affect intracellular processes mediated through the enzyme or the second messenger.