Patent ID: 6555659
Filing Date: 2003-04-29
Classification: A23J,C07K,Y10S

Abstract:
A process for the purification of glycomacropeptide (GMP), comprising:contacting a feedstock containing GMP with a first anion exchanger under conditions to adsorb said GMP; obtaining an eluate containing GMP by eluting said adsorbed GMP from said anion exchanger with an eluent; removing impurities from said eluate containing GMP, wherein said impurities comprise other whey proteins, by a technique selected from the group consisting of: a) contacting said eluate containing GMP with a cation exchanger in conditions under which said impurities in said eluate are adsorbed onto said cation exchanger, and precipitating said impurities in said eluate containing GMP using conditions by means of which said GMP remains in solution, b) precipitating said impurities in said eluate containing GMP using conditions by means of which said GMP remains in solution, c) contacting said eluate containing GMP with a second anion exchanger in conditions under which said impurities in said eluate are adsorbed onto said anion exchanger, and d) contacting said eluate containing GMP with a cation exchanger in conditions under which said impurities in said eluate are adsorbed onto said cation exchanger, and contacting said eluate containing GMP with a second anion exchanger in conditions under which said impurities in said eluate are adsorbed onto said anion exchanger; and recovering a purified GMP, wherein said purified GMP has an amino acid composition containing not greater than 0.5% (w/w) phenylalanine.