Patent ID: 6010875
Filing Date: 2000-01-04
Classification: C07K,C12N

Abstract:
A double-stranded DNA plasmid which upon introduction into an Eschericia coli host cell renders the host cell capable of effecting expression of DNA encoding a desired eukaryotic polypeptide comprising in 5' to 3' order the following:a) DNA which includes in 5' to 3' order the tandem promoters deo P1 and deo P2;b) DNA which includes a ribosomal binding site for rendering mRNA transcribed from the DNA encoding the polypeptide capable of binding to ribosomes within the host cell;c) an ATG initiation codon; andd) DNA encoding the polypeptide in phase with the ATG initiation codon;and which additionally includes a DNA sequence comprising an origin of replication from a bacterial plasmid capable of autonomous replication in the host cell and a DNA sequence which contains a gene associated with a selectable or identifiable phenotypic trait which is manifested when the plasmid is present in the host cell.