Patent ID: 6627402
Filing Date: 2003-09-30
Classification: C12Q

Abstract:
A method for identifying a polymorphic nucleotide in a target nucleic acid sequence in a nucleic acid sample, said method comprising(a) combining under hybridizing conditions to form a duplex (i) a nucleic acid sample which may include a target nucleic acid sequence, (ii) a primer having a 3â€² portion and a 5â€² portion wherein said 3â€² portion is complementary to a template sequence immediately adjacent a polymorphic nucleotide in a target nucleic acid sequence, and wherein said 5â€² portion is complementary to a preselected nucleic acid sequence which is different from said target nucleic acid sequence and which said preselected nucleic acid sequence is immobilized at a preselected location in an array of immobilized, preselected nucleic acid sequences on a solid support, wherein the length, base composition and percentage of each base in each of said immobilized preselected nucleic acid sequences are substantially the same to permit hybridization between said 5â€² portion and its complementary preselected nucleic acid sequence to be carried out under substantially the same conditions at each location on the array, (b) adding a template dependent polymerase and a labeled nucleoside triphosphate which is specific for the polymorphic nucleotide, to the duplex of step (a), if present, under conditions to form a labeled primer extension product which includes the primer of (a)(ii), (c) screening the labeled primer extension product of (b) by combining the 5â€² portion of the primer with the array of immobilized, preselected nucleic acid sequences on the solid support whereby the label identifies the polymorphic nucleotide and the location of the immobilized preselected nucleic acid sequence is associated with the locus of the target nucleic acid sequence in the nucleic acid sample.