Patent ID: 7964345
Filing Date: 2011-06-21
Classification: C12Q

Abstract:
1. A method for detecting whether a reciprocal chromosomal translocation, involving a first potential breakpoint associated with the reciprocal translocation on a first chromosome and a second potential breakpoint associated with the reciprocal translocation on a second chromosome, is present in a chromosomal preparation, comprising: (a) providing first, second, third and fourth nucleic acid probes respectively labeled with first, second, third and fourth labels, the first and second probes hybridizing to the first chromosome and producing a spot generated from the first label and a spot generated from the second label on the first chromosome, the third and fourth probes hybridizing to the second chromosome and producing a spot generated from the third label and a spot generated from the fourth label on the second chromosome, the first and second chromosomes being susceptible to undergoing a reciprocal translocation with each other, wherein the first, second, third and fourth probes are different and the first, second, third and fourth labels are different; the first probe hybridizing to a segment of DNA located between the first chromosome's centromere (FCC) and the first potential breakpoint, the second probe hybridizing to a segment of DNA located between the first chromosome's telomere (FCT) and the first potential breakpoint, the third probe hybridizing to a segment of DNA located between the second chromosome's centromere (SCC) and the second potential breakpoint, the fourth probe hybridizing to a segment of DNA located between the second chromosome's telomere (SCT) and the second potential breakpoint; (b) contacting the chromosomal preparation with the first, second, third and fourth nucleic acid probes under hybridization conditions; and (c) detecting whether there is/are one or more hybridizations of the first, second, third and fourth probes to the chromosomal preparation, wherein the existence of the one or more hybridizations of the first, second, third and fourth probe to the chromosomal preparation results in one or more spots generated from the first, second, third and fourth labels on one or more chromosomes of the chromosome preparation; wherein