Patent ID: 8673569
Filing Date: 2014-03-18
Classification: C12Q,Y02A

Abstract:
1. A method of assaying for a nucleic acid sequence, the method comprising contacting a split primer with a sample that may comprise DNA derived from a Dengue virus under conditions necessary for the amplification, wherein the split primer has two regions comprising a first sequence complementary to a first region of a target nucleic acid sequence and a second sequence complementary to a second region of a target nucleic acid, and wherein the two regions of the split primer are not separated by an intervening base, and wherein the split primer targets two or more unique locations on a target nucleic acid from the Dengue virus, and wherein the two or more unique locations are separated by at least one nucleic acid base, and wherein amplification of the target sequence is carried out in the presence of a polymerase and a reverse primer under conditions necessary for amplification or extension, and is indicative of the presence of DNA derived from Dengue virus in the sample.