Patent ID: 7208284
Filing Date: 2007-04-24
Classification: C12Q,G01N

Abstract:
1. A method for measuring polyamines in erythrocytes, comprising: forming hydrogen peroxide by reacting an eluate of erythrocytes separated and purified from blood with at least one polyamine oxidase having a substrate specificity to spermine and spermidine, and measuring the hydrogen peroxide formed by reacting the hydrogen peroxide using at least one diphenylamine-based highly sensitive chromogen to measure a total amount of spermine and spermidine in the erythrocytes, wherein said polyamine oxidase reacts with 1 mol of spermine to relase 1 mol of hydrogen peroxide and reacts with 1 mol of spermidine to release 1 mol of hyrdrogen peroxide, and wherein said polyamine oxidase is an enzyme derived from a gramineous plant.