Patent ID: 9150894
Filing Date: 2015-10-06
Classification: C07K,C12P

Abstract:
1. A process for purifying daptomycin comprising a) loading a solution comprising partly purified daptomycin onto an anion exchange chromatography column and eluting a first eluate with a first elution buffer; wherein the partially purified daptomycin is optionally clarified from a fermentation broth comprising daptomycin using one or several clarification steps; b) loading the first eluate of step a) onto a first reverse phase chromatography column and eluting a second eluate with a second elution buffer having a pH of 6.5-8.5; c) loading the second eluate of step b) onto a second reverse phase chromatography column and eluting a third eluate with a third elution buffer having a pH of 2.5-3.5; and d) optionally filtering and lyophilizing the third eluate; wherein the first elution buffer in a) is a monovalent salt solution, the second elution buffer in b) consists of water and an alcohol, and the third elution buffer in c) consists of water, an alcohol and an acid.