Patent ID: 7972603
Filing Date: 2011-07-05
Classification: A61K,A61P

Abstract:
1. A stable immunogenic product for inducing antibodies raised against a TNFα protein in a subject, the immunogenic product comprising protein immunogenic heterocomplexes comprising TNFα protein molecules associated with KLH carrier protein molecules, more than 1% and less than 40% of the TNFα protein molecules are directly covalently linked to the KLH carrier protein molecules, and more than 60% of the TNFα protein molecules are non-covalently associated with the KLH carrier protein, wherein said stable immunogenic product is produced by a process comprising the following steps: a) incubating TNFα proteins and KLH carrier molecules in a molar ratio TNFα:KLH ranging from 10:1 to 50:1 in the presence of glutaraldehyde to produce immunogenic heterocomplexes; b) removing excess glutaraldehyde; c) stabilizing the immunogenic heterocomplexes with formaldehyde; d) adding glycine to block the reaction with formaldehyde in step c); e) performing a dialysis of the product obtained at step d); and f) collecting the product comprising immunogenic heterocomplexes prepared at step e).