Patent ID: 6090567
Filing Date: 2000-07-18
Classification: C12N,C12Q,G01N

Abstract:
A method for homogeneous immunoassay of an analyte in a sample suspected of containing said analyte which comprises: (1) combining in a liquid medium: (a) said sample, (b) a conjugate of an analog of said analyte with a mutant bacterial NAD.sup.+ dependent G6PDH derived from the bacterial genus Leuconostoc and differing from any precursor G6PDH by the deletion of, or substitution for, at least one lysine amino acid per subunit, or substitution of, or insertion of, at least one cysteine amino acid per subunit, (c) a receptor for said analyte, and (d) substrates for said G6PDH; (2) determining the enzymatic activity of said G6PDH in said medium; and (3) comparing said activity to the enzymatic activity observed with a sample containing said analyte.