Patent ID: 6355415
Filing Date: 2002-03-12
Classification: C12Q

Abstract:
A method for detecting changes in a zebrafish cell or in tissue obtained from a zebrafish cell, said changes caused by cleaving mRNA encoded by a gene of interest, comprising:a) providing: i) a zebrafish cell comprising a zebrafish genomic sequence having homology to a human genomic sequence, said zebrafish genomic sequence encoding an RNA sequence containing a substrate cleavage sequence; and ii) a ribozyme sequence capable of cleaving said RNA sequence; b) introducing said ribozyme sequence into said zebrafish cell to generate a manipulated cell, wherein said introducing is under conditions such that said RNA sequence is cleaved by said ribozyme sequence; and c) detecting one or more changes selected from morphological changes and behavioral changes in said manipulated cell relative to said zebrafish cell, or in tissue obtained from said manipulated cell relative to tissue obtained from said zebrafish cell, wherein said behavioral chantges are selected from light tropism and chemical tropism.