Patent ID: 6258604
Filing Date: 2001-07-10
Classification: C07K,C12N

Abstract:
A gram-positive bacterium transformed with a recombinant vector, wherein the vector comprises:(A) a DNA fragment that promotes, in gram-positive bacteria, replication of said recombinant vector, wherein said DNA fragment is selected from the group consisting of(1) a nucleotide sequence of SEQ ID NO:4 from Bacillus thuringiensis of about 2.6 kb and(2) a fragment included in SEQ ID NO:4, provided that said fragment allows the replication of the recombinant vector when it is under the control of a functional promoter in the gram-positive bacteria, and(B) a DNA sequence of interest operably linked to (A); wherein said vector does not comprise a fragment of the BalI--BalI fragment of SEQ ID NO:1 other than the DNA fragment of (A).