Patent ID: 6303115
Filing Date: 2001-10-16
Classification: A61P,C12Q

Abstract:
A method of screening a plurality of test compounds for a test compound that decreases or increases the proliferation of a strain in a pool of strains, comprising:providing a pool of strains, each strain differing from each other strain in a sequence of at least one gene, wherein each strain further comprises a distinguishable nucleic acid sequence tag, wherein each tag is distinct from the gene in that strain;culturing the pool of strains in the presence of each test compound of the plurality of test compounds; and,determining whether the presence of the test compound decreases or increases the representation of a distinguishable nucleic acid sequence tag,wherein a decrease in the representation of a distinguishable nucleic acid sequence tag indicates a decrease in the representation of a tagged strain and a decrease in the representation of a tagged strain indicates that the test compound decreases the proliferation of that strain, and an increase in the representation of a distinguishable nucleic acid sequence tag indicates an increase in the representation of a tagged strain and an increase in the representation of a tagged strain indicates that the test compound increases the proliferation of that strain.