Patent ID: 7829347
Filing Date: 2010-11-09
Classification: G01N,Y10S

Abstract:
1. A diagnostic test kit for detecting the presence or quantity of a test analyte within a test sample, the diagnostic test kit comprising: detection probes conjugated with a first specific binding member that is configured to preferentially bind to the test analyte; calibration probes conjugated with a second specific binding member that is configured to preferentially bind to a calibration analyte; and a lateral flow assay device on which the detection probes and calibration probes are disposed, the lateral flow assay device comprising a porous membrane, the porous membrane defining: a detection zone in which is immobilized a first receptive material, the first receptive material being configured to preferentially bind to the test analyte; an indicator zone in which is immobilized a second receptive material, the second receptive material being configured to preferentially bind to uncomplexed conjugated detection probes; and a calibration zone in which is immobilized a third receptive material, the third receptive material being configured to preferentially bind to the calibration analyte; wherein the lateral flow device further comprising a conjugate pad in fluid communication with the porous membrane and a sample pad that is positioned upstream from the conjugate pad, wherein the detection probes and calibration probes are disposed on the conjugate pad and wherein the calibration analyte is disposed on the sample pad.