Patent ID: 7005265
Filing Date: 2006-02-28
Classification: B01J,C12Q

Abstract:
1. A device for nonenzymatically amplifying the signal from a hybridization reaction between at least a first nucleic acid probe and its cognate nucleic acid target present in fluid solvent, the device comprising: at least one substrate; a first plurality of probe complexes, each having a probe nucleic acid strand and a displacement nucleic acid strand hybridized thereto, the plurality of probe strands being identical and the plurality of displacement strands being identical; and a first plurality of identical docking nucleic acid strands, wherein the displacement strands and the docking strands are immobilized to one of said at least one substrates, said immobilized displacement strands and said immobilized docking strands being spatially segregated from one another, separately addressable, and fluidly communicating upon contact of said device with fluid solvent, wherein the probe strand of said probe complexes has at least three regions, whereby contact of target to said device is capable of catalyzing transfer of probe strands from the displacement strands to the spatially segregated, separately addressable docking strands, the transfer of said probe generating a detectable signal.