Patent ID: 6200800
Filing Date: 2001-03-13
Classification: A61K,A61P,C12N

Abstract:
A retroviral vector comprising a nucleic acid molecule comprising:(A) retroelements that comprise a recombinant provirus when a target cell is infected by a retrovirus containing said retroelements;(B) a nucleotide sequence of interest, which can be expressed in the target cell and which can be transferred with said retroelements into the target cell and integrated into the recombinant provirus; and(C) a recognition sequence for the elimination of proviral sequences in the recombinant provirus, which are not necessary for expression of the nucleotide sequence of interest in the target cell after integration of the recombinant provirus into the target cell;wherein the vector is a defective Moloney murine leukemia provirus comprising said recognition sequence in a U3 region of its 3' LTR, a U5 region of its 5' LTR, or an R region of said provirus.