Patent ID: 8551725
Filing Date: 2013-10-08
Classification: C12Q,G01N

Abstract:
1. A method of identifying an agent that modulates γ-proteobacterium long chain acyl homoserine lactone (AHL) acylase activity, comprising: a) contacting at least one sample comprising PA1032 AHL acylase as set forth in SEQ ID NO: 5 and a long chain AHL with at least one test agent, under conditions suitable for AHL acylase activity, wherein the PA1032 AHL acylase is truncated from about 1 to 6 amino acids at the NH terminus and comprises an alternate methionine initiator residue; and b) measuring AHL acylase activity in the presence of the test agent; wherein a change in AHL acylase activity as compared to the AHL acylase activity in the absence of the test agent identifies the test agent as an agent that modulates the γ-proteobacterium long chain AHL acylase activity.