Patent ID: 6110666
Filing Date: 2000-08-29
Classification: A01K,A61K,C07K,C12N

Abstract:
A method for identifying a locus control subregion (LCR) subregion comprising a chromatin opening domain, said method comprising the steps of;a) providing a host cell containing a DNA construct in single copy integrated into the host cell genome at a site of integration, the construct comprising a candidate LCR subregion comprising a chromatin opening domain operably linked to an expressible reporter gene, said candidate LCR subregion consisting of a single DNase I hypersensitive site; andb) determining that the DNA construct reproducibly actuates transcription in said host cell that is restricted to said host cell type and independent of the site of integration of said construct in the host cell genome,wherein the presence of an LCR subregion comprising a chromatin opening domain is indicated by reproducible actuation of transcription of said reporter gene in single copy in a host cell.