Patent ID: 6214538
Filing Date: 2001-04-10
Classification: C12Q

Abstract:
A method of distinguishing between serotypes and/or detecting variants of infectious bronchitis virus comprising:a. amplifying the S1 gene region of infectious bronchitis virus;b. digesting the amplified S1 gene region with HaeIII, or a restriction endonuclease which cleaves at the HaeIII restriction site, to form a first set of restriction fragments;c. digesting the amplified S1 gene region with XcmI, or a restriction endonuclease which cleaves at the XcmI restriction site, to form a second set of restriction fragments;d. digesting the amplified S1 gene region with BstYI, or a restriction endonuclease which cleaves at the BstYI restriction site, to form a third set of restriction fragments;e. separating the restriction fragments within the first, second and third sets by electrophoresis; andf. analyzing restriction fragment length polymorphisms to distinguish between serotypes or detect variants of infectious bronchitis virus.