Patent ID: 8399209
Filing Date: 2013-03-19
Classification: G01N,Y10S

Abstract:
1. A homogeneous method for quantitatively or qualitatively detecting an analyte in a sample (sample analyte) comprising (1) contacting the sample with wherein at least one of the conjugates comprising R1 or R2 possesses more than one binding site for the respective specific binding partner X or Y; wherein the first and second components of the signal-generating system enter into a detectable interaction when spatially close to each other and the detectable interaction comprises a direct energy transfer between the components; wherein (i) both R1 and R2 are able to bind specifically to the sample analyte, or (ii) only R1 is able to bind specifically to the sample analyte and R2 can compete with the sample analyte in binding to R1; and (2) detecting the analyte in the sample (sample analyte) by measuring the magnitude of the interaction between the components of the signal-generating system; wherein said method is a homogeneous binding test.