Patent ID: 8802391
Filing Date: 2014-08-12
Classification: B01L,G01N

Abstract:
1. A method for measuring the transmigration of cells in an assembly comprising a sample channel and an analyte channel or chamber which are in fluid communication in an overlap region between the sample channel and the analyte channel or chamber, said sample channel being separated from said analyte channel or chamber in said overlap region by a semi-permeable membrane mounted between the sample channel and the analyte channel or chamber, the method including: introducing a reagent into the analyte channel or chamber mixed with a gel which is a solid gel or is initially in the form of a liquid that is allowed to solidify in the analyte channel or chamber, delivering sample cells in a liquid carrier medium at a controlled flow rate through the sample channel on one side of the semi-permeable membrane whereby said sample cells are separated from the reagent in the analyte channel or chamber by the semi-permeable membrane, and measuring the transmigration of sample cells from the sample channel through the semi-permeable membrane into the analyte channel or chamber under the influence of the reagent in the analyte channel or chamber.