Patent ID: 7070941
Filing Date: 2006-07-04
Classification: G01N,Y10S,Y10T

Abstract:
1. A method for detecting at least a first isoprenylated protein in a cell comprising: a) obtaining a substrate of at least a first protein in said cell, wherein the substrate is a synthetic isoprenyl azide substrate comprising at least a first azide; b) contacting the cell with the synthetic isoprenyl azide substrate under conditions wherein the cell takes up synthetic isoprenyl azide substrate and the synthetic isoprenyl azide substrate reacts with the first protein to produce at least a first isoprenylated protein; and c) detecting at least said first isoprenylated protein from proteins produced by said cell by contacting the proteins produced by said cell with a phosphine capture reagent, wherein capture occurs by the Staudinger reaction.