Patent ID: 6451306
Filing Date: 2002-09-17
Classification: A61K,C07K,C12N

Abstract:
A method for intraparenchymal delivery of a therapeutic nerve growth factor to cells in the primate brain to treat basal forebrain cholinergic neuron dysfunction in Alzheimer's Disease, the method comprising grafting donor cells into multiple grafting sites in the cholinergic basal forebrain of a human or non-human primate, wherein the donor cells are genetically modified by insertion therein of at least one transgene encoding a nerve growth factor; and wherein the donor cells are grafted into each grafting site in a pharmaceutically acceptable fluid composition having a concentration of donor cells of at least 1Ã—105 cells/&mgr;l fluid; and wherein the donor cells are delivered to each grafting site in a dosage of between 6 and 20 &mgr;l; and wherein further each grafting site is no more than about 500 &mgr;m from a defective, diseased or damaged brain cell and no more than about 5 mm from any other grafting site.