Patent ID: 6077663
Filing Date: 2000-06-20
Classification: A61K,A61P,C07K,C12N,Y10T

Abstract:
A composition for the transfection of a higher eukaryotic cell which comprises a non-naturally occurring nucleic acid complex, wherein said nucleic acid complex comprises(a) one or more nucleic acids of interest;(b) a substance having affinity for nucleic acid, wherein said substance is optionally coupled with an internalizing factor for said higher eucaryotic cell; and(c) an endosomolytic agent which has the ability of being internalized into said higher eucaryotic cell, either per se or as a component of the nucleic acid complex,wherein said endosomolytic agent has the ability to release contents of the endosome, in which the nucleic acid complex is located after entering the higher eucaryotic cell, into the cytoplasm of said higher eucaryotic cell, and wherein said endosomolytic agent is selected from the group consisting of: a virus; a viral component; a non-viral optionally modified natural or synthetic peptide; a fusion of naturally occurring and artificial peptide sequences; and a non-peptidic amphipathic substance.