Patent ID: 6818427
Filing Date: 2004-11-16
Classification: C07H,C12N

Abstract:
An isolated nucleic acid selected from the group consisting of:a. a nucleic acid comprising the nucleotide sequence of SEQ ID NO: 1 or nucleotides 7-4545 of SEQ ID NO: 1; b. a nucleic acid comprising the nucleotide sequence of the cDNA insert of the plasmid deposited with the ATCC as Accession Number PTA-1836 or a potion thereof, comprising the coding region; c. a nucleic acid which encodes a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:2, wherein said fragment comprises at least 500 contiguous amino acids of SEQ ID NO:2 and has MEKK1 activity; d. a nucleic acid which has at least 90% nucleotide sequence identity with the entire length of the nucleotide sequence of SEQ ID NO: 1, wherein said nucleic acid comprises nucleotide residues 1 to 64 of SEQ ID NO: 1 and wherein said nucleic acid encodes for a protein having MEKK1 activity; e. a nucleic acid which has at least 90% nucleotide sequence identity with the entire length of the nucleotide sequence of the insert of the plasmid deposited with the ATCC as Accession Number PTA-1836, wherein said nucleic acid comprises nucleotide residues 1 to 64 of SEQ ID NO:1 and wherein said nucleic acid encodes for a protein having MEKK1 activity; f. a nucleic acid comprising the nucleotide sequence which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2 or the amino acid sequence encoded by the cDNA insert of the plasmid deposited with ATCC as Accession Number PTA-1836; g. a nucleic acid encoding a MEKK1 variant, wherein said MEKK1 variant comprises an amino acid sequence, having at least 90% amino acid sequence identity with the entire length of SEQ ID NO:2 wherein said nucleic acid encodes for amino acid residues 1 to 20 of SEQ ID NO:2, and wherein said variant has MEKK1 activity; and h. a nucleic acid encoding a MEKK1 variant, wherein said MEKK1 variant comprises an amino acid sequence having at least 98% amino acid sequence identity with the entire length of SEQ ID NO:2 and wherein said variant has MEKK1 activity.