Patent ID: 8623614
Filing Date: 2014-01-07
Classification: A61K,B82Y,C07K

Abstract:
1. A method for the recombinant production of magnetic nanoparticles, comprising: (a) providing one or more cells capable of producing magnetic nanoparticles, comprising one or more genes having a modified expression as compared to the expression of the corresponding one or more wild-type genes, (b) cultivating the cells; and (c) isolating the magnetic nanoparticles from the cultivated cells, wherein the one or more genes are selected from the group consisting of the mamG, mamF, mamD, and mamC genes of wherein the modified expression results from a deletion in the one or more cells of the one or more genes, an introduction into the one or more cells of a nucleic acid molecule comprising a nucleotide sequence encoding one or more copies of the one or more genes, or combinations thereof; wherein the nucleotide sequence encodes a genetic construct selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, and SEQ ID NO: 14; wherein the magnetic nanoparticles have a defined size; and wherein said defined size varies depending on the type and the extent of the modification of gene expression performed.