Patent ID: 6030527
Filing Date: 2000-02-29
Classification: B01D

Abstract:
A system for separating a mixture of polynucleotide fragments comprising a chromatographic column having two ends, said column containing a separation bed of Matched Ion Polynucleotide Chromatography separation particles held in the column between porous frits positioned at each end thereof, said column having an inlet, an injection valve in communication with said inlet through a flow path therebetween, mobile phase supply means in communication with said injection valve through at least one flow path therebetween, and multivalent cation capture resin, selected from cation exchange resin and chelating resin, positioned in said flow path between said inlet and said injection valve, said multivalent cation capture resin being capable of removing multivalent cations from aqueous solutions, whereby any multivalent cation contaminants in said flow path are removed before said contaminants contact the separation bed wherein said injection valve communicates with the inlet of said separation column through a conduit, and said mobile phase supply means communicates with said injection through at least one conduit, and wherein said porous frits, chromatographic column, injection valve, mobile phase supply means, and conduits have process solution-contacting surfaces which contact process solutions held therein or flowing therethrough, and wherein the process solution-contacting surfaces of said chromatographic column, injection valve, mobile phase supply means, and conduits are material which does not release multivalent cations into aqueous solutions held therein or flowing therethrough.