Patent ID: 7846727
Filing Date: 2010-12-07
Classification: C12N

Abstract:
1. A substratum having a pattern of a cell-culture controlling substance on a base, said substratum prepared by a process comprising: a step of applying a droplet of a first liquid, the first liquid containing a first cell-culture controlling substance, onto a surface of the base; a step of applying a droplet of a second liquid, the second liquid containing a second cell-culture controlling substance, onto the base surface so as to overlap the droplet of the first liquid; and a step of immobilizing the first and second cell-culture controlling substances on the base, wherein the first and second cell-culture controlling substances are able to control at least one function selected from the group consisting of proliferation, differentiation, survival, maintenance of undifferentiated state and apoptosis of cells, one of the first and second cell-culture controlling substances being a cytokine, and wherein a region where the first and second cell-culture controlling substances are immobilized has a width of less than two-fold the width of a cell to be cultured on the substratum.