Patent ID: 7030217
Filing Date: 2006-04-18
Classification: C07K

Abstract:
1. A kit for preparing assembled polypeptides comprising: (a) a first container containing a solid support, having bound thereto a first partially or completely unprotected peptide having an N-terminus and a thioacid or a thioester of the formula —COSR at its C-terminus and, wherein said partially or completely unprotected peptide is bound to said solid support via a linker; wherein said linker comprises a cleavable moiety and said partially or completely unprotected first peptide segment is bound to said linker at said N-terminus, and wherein R is a straight or branched C (b) a second container containing a set of second partially or completely unprotected peptides, each comprising a thioester or a thioacid at its C-terminus and a cysteine residue at its N-terminus; wherein the N-terminal cysteine of said second peptide segment is capable of selectively ligating to the C-terminus of said solid phase-bound first peptide to form a solid phase-bound peptide comprising a thioacid at its C-terminus; and (c) one or more additional containers each containing a set of different partially or completely unprotected peptide segments, each comprising a thioester or a thioacid at its C-terminus and a cysteine residue at its N-terminus, wherein each of the members of each set have the same number of amino acids.