Patent ID: 6828095
Filing Date: 2004-12-07
Classification: C07K,C12N,C12Q

Abstract:
A method for identifying a candidate compound for the ability to modulate expression of OP-1, said method comprising the steps of:(a) incubating a candidate compound with a cell co-transfected with vectors comprising: (i) a DNA sequence encoding a reporter gene in operative association with at least one OP-1-specific non-coding sequence which can modulate expression of said reporter gene, wherein said non-coding sequence comprises a first Pax-responsive OP-1 modulating element which is responsive to a first Pax gene expression product; and (ii) a DNA sequence encoding a first Pax gene expression product; (b) measuring the level of reporter gene expressed in said cell; and (c) comparing said level with that of said reporter gene expressed in said cell in the absence of said candidate compound, wherein a change in reporter gene expression level is indicative of said candidate's ability to modulate OP-1 expression.