Patent ID: 7807815
Filing Date: 2010-10-05
Classification: A01K,A61K,A61P,C12N

Abstract:
1. A nucleic acid-lipid particle comprising: (a) a modified siRNA comprising a double-stranded sequence of about 15 to about 30 nucleotides in length, said sequence comprising a non-immunostimulatory mismatch motif relative to an unmodified siRNA sequence that is capable of silencing expression of a target sequence, wherein the mismatch motif consists of a 5′-XX′-3′ dinucleotide corresponding to a 5′-GU-3′ dinucleotide in the sense or antisense strand of the unmodified siRNA sequence, wherein X and X′ are independently selected from the group consisting of A, U, C, and G, with the proviso that if X is G, X′ is not U and if X′ is U, X is not G, wherein the modified siRNA is less immunogenic than the unmodified siRNA sequence, and wherein the modified siRNA is capable of silencing expression of the target sequence; (b) a cationic lipid, wherein said cationic lipid is selected from the group consisting of 1,2-DiLinoleyloxy-N,N-dimethylaminopropane (DLinDMA), 1,2-Dilinolenyloxy-N,N-dimethylaminopropane (DLenDMA), and a mixture thereof; (c) a non-cationic lipid; and (d) a conjugated lipid that inhibits aggregation of particles.