Patent ID: 6197510
Filing Date: 2001-03-06
Classification: C12Q

Abstract:
A method of sequencing a nucleic acid comprising:(a) providing in a reaction mixture first and second nucleic acid target sequences;(b) providing in the reaction mixture first and second labelled sequencing primers hybridizable respectively to the first and second nucleic acid target sequences, the second sequencing primer being at least as long as the total length of the first sequencing primer plus the length of the first target sequence;(c) providing in the reaction mixture a DNA polymerase, deoxyribonucleotides, a chain-terminating deoxyribonucleotide, and conditions that permit duplication of the first and second target sequences by the polymerase to proceed by extension from the first and second sequencing primers respectively, wherein the periodic incorporation of the chain-terminating nucleotide by the polymerase terminates polymerization to produce a pool of primer extension products of various lengths, each of the primer extension products in the pool beginning with the second sequencing primer being longer than the primer extension products in the pool beginning with the first sequencing primer; and,(d) differentiating the primer extension products based on differences of molecular weight;wherein at least one nucleic acid sequence, or at least one portion of a nucleic acid sequence, is determined.