Patent ID: 8945361
Filing Date: 2015-02-03
Classification: C07K,G01N

Abstract:
1. A method of determining the isoelectric point of one or more analytes by isoelectric focusing comprising the steps of: forming a pH gradient in a fluid path including one or more analytes and one or more standards, the one or more standards including one or more moieties, at least one of the one or more moieties being a at least one reactive moiety, when the at least one reactive moiety is activated to form an activated moiety, the activated moiety attaches the one or more standards to a substrate; focusing the one or more analytes at a position equal to the isoelectric point of each analyte from the one or more analytes and focusing the one or more standards at a position equal to the isoelectric point of each standard from the one or more standards; immobilizing the one or more analytes and the one or more standards in said fluid path by activating the at least one reactive moiety, the immobilizing being non-specific to any analyte from the one or more analytes; detecting one or more signals generated from the one or more analytes and the one or more standards; and determining the isoelectric point of each analyte from the one or more analytes by comparing the signals of the one or more analytes to the signals of the one or more standards.