Patent ID: 7618621
Filing Date: 2009-11-17
Classification: A61K,A61P,C12N

Abstract:
1. One or a plurality of mammalian multipotent neural stem cells that can form a cluster produced according to the method comprising the steps of: a. isolating mammalian neural stem cells from mammalian neural tissue containing at least one neural stem cell, b. culturing the neural stem cells in a cell culture media under conditions that permit proliferation of the neural stem cells in vitro, in the presence of substituted deoxynucleotide or deoxynucleoside in an amount effective to adapt the cells to proliferate, migrate and differentiate when transplanted into host tissue and at least one growth factor, wherein cells are cultured in an uncoated flask or a flask that has been treated to repel the cells, and wherein the substituted deoxynucleotide or deoxynucleoside is bromodeoxyuridine, bromodeoxycytidine, bromodeoxyguanosine, methyldeoxythymidine, or chlorodeoxyuridine, to generate the one or a plurality of mammalian multipotent neural stem cells that can form a cluster, and c. thereafter harvesting the one or plurality of mammalian multipotent neural stem cells that can form a cluster produced in (b).