Patent ID: 8822160
Filing Date: 2014-09-02
Classification: C12Q,G01N,Y10T

Abstract:
1. A plurality of transmembrane protein pores, each covalently attached to a cyclodextrin molecular adaptor that facilitates an interaction between the pore and a nucleic acid analyte, wherein: (i) the pores are variants of α-hemolysin in which one or more of the subunits has at least 50% homology to SEQ ID NO: 2 based on amino acid identity over the entire sequence and which retain pore activity; (ii) the adaptors in the plurality of pores are collectively orientated so that either (a) the primary hydroxyls or amino groups of the adaptors face the trans entrance of the pore or (b) the secondary hydroxyls or amino groups of the adaptors face the trans end of the pores; (iii) the pores are modified at position 113 in one or more subunits to facilitate the orientation of the adaptors, and (iv) the pores are modified at position 113 or 117 in one or more subunits to facilitate the covalent attachment of the adaptors.