Patent ID: 6069005
Filing Date: 2000-05-30
Classification: A61K,C12N,G01N

Abstract:
A method of isolating hepatic progenitors from adult liver comprising:(a) preparing a single cell suspension of adult liver cells;(b) panning said suspension utilizing antibodies specific for mature hepatocytes, mature bile duct cells, endothelial cells, mesenchymal cells, and hemopoietic cells so as to remove mature hepatocytes, mature bile duct cells, endothelial cells, mesenchymal cells, and hemopoietic cells from said suspension; and(c) performing fluorescence activated cell sorting utilizing said antibodies so as to remove remaining mature hepatocytes, mature bile duct cells, endothelial cells, mesenchymal cells, and hemopoietic cells from said suspension; and(d) performing multiparametric fluorescence activated cell sorting on said suspension utilizing at least one antibody to a hepatic cell marker, side scatter, forward scatter and/or autofluorescence to isolate said hepatic progenitors.