Patent ID: 8138322
Filing Date: 2012-03-20
Classification: C07K,C12Q,G01N,G16B

Abstract:
1. An isolated nucleic acid that encodes a T1R2 taste receptor polypeptide or T1R2 extracellular or T1R2 cytoplasmic domain wherein said nucleic acid is selected from the group consisting of (i) the nucleic acid of SEQ ID NO 23; (ii) a nucleic acid encoding a polypeptide having at least 90% sequence identity to the T1R2 polypeptide in SEQ ID NO:21 or an extracellular or cytoplasmic domain thereof; (iii) a nucleic acid s encoding a human T1R2 polypeptide or a T1R2 cytoplasmic or extracellular domain wherein said nucleic acid hybridizes under stringent hybridization conditions to the complement of the nucleic acid sequence contained in SEQ ID NO: 23 and wherein said stringent hybridization conditions are hybridization in 50% formamide, 5 times SSC and 1% SDS, incubating at 42 degrees C., with wash in 0.2 times SSC and 0.1% SDS at 65 degrees C., wherein the hybridization and wash steps are each carried out for at least 1 minute.