Patent ID: 7037695
Filing Date: 2006-05-02
Classification: C07K,C12Q,G01N

Abstract:
1. A method for identifying agents that increase binding between a Wolframin protein polypeptide, comprising an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:8, and SEQ ID NO: 10, and a carboxypeptidase E binding partner polypeptide comprising an amino acid sequence selected from the group consisting of SEQ ID NO:4, SEQ ID NO 12, SEQ ID NO: 14, SEQ ID NO: 15, and SEQ ID NO:17 comprising: (a) contacting said Wolframin protein polypeptide and said carboxypeptidase E binding partner polypeptide in the presence and absence of a test agent; (b) determining binding between said Wolframin protein polypeptide and said binding partner polypeptide in the presence and absence of the test agent; and (c) comparing binding between said Wolframin protein polypeptide and said carboxypeptidase E binding partner polypeptide in the presence and absence of the test agent to determine whether binding between said Wolframin protein polypeptide and a said carboxypeptidase E binding partner polypeptide is increased in the presence of the test agent.