Patent ID: 6174683
Filing Date: 2001-01-16
Classification: G01N

Abstract:
A method of preparing a biochip having an organic solvent soluble biomolecule covalently bound thereto, the method comprising:a) providing a solid substrate having a top surface with reactive molecules thereon;b) providing a solution comprising the biomolecule in an aprotic organic solvent;c) providing a polyurethane-based hydrogel prepolymer in an aprotic organic solvent;d) derivatizing the polyurethane-based hydrogel prepolymer with the biomolecule from step (b);e) initiating polymerization of the derivatized polyurethane-based hydrogel prepolymer of step (d) by adding a buffered aqueous solution thereto; andf) microspotting the polymerizing hydrogel solution of step (e) onto the top surface of the substrate of step (a), such that the polymerizing hydrogel reacts with the reactive molecules on the surface of the substrate resulting in covalent binding of the hydrogel to the substrate.