Patent ID: 9115378
Filing Date: 2015-08-25
Classification: C12N,C12P

Abstract:
1. A process for the fermentative production of Vitamin C, comprising: cultivating a host cell selected from the group consisting of Gluconobacter, Gluconacetobacter and Acetobacter to allow the direct production of Vitamin C from a carbon source obtainable from D-glucose or D-sorbitol metabolization pathway, wherein the genome of said host cell is genetically engineered with a polynucleotide encoding L-sorbosone dehydrogenase selected from the group consisting of: (i) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:1; (ii) a polynucleotide encoding L-sorbosone dehydrogenase that has the ability to directly convert L-sorbosone into Vitamin C comprising the amino acid sequence of SEQ ID NO:2; (iii) a polynucleotide that hybridizes under highly stringent conditions to a polynucleotide encoding L-sorbosone dehydrogenase that has the ability to directly convert L-sorbosone into Vitamin C comprising the amino acid sequence of SEQ ID NO:2, and wherein said highly stringent conditions comprise hybridization at 42° C. for 2 to 4 days followed by two washes in 2×SSC, 0.1% SDS at room temperature and two washes in 0.5×SSC, 0.1% SDS or 0.1×SSC, 0.1% SDS at 65° C. to 68° C.; and (iv) a polynucleotide that is at least 95% identical to a polynucleotide encoding L-sorbosone dehydrogenase that has the ability to directly convert L-sorbosone into Vitamin C comprising the amino acid sequence of SEQ ID NO:2, and wherein a polynucleotide that encodes a Sorbitol/Sorbose Metabolization System (SMS) protein having the amino acid sequence of SEQ ID NO:137 is overexpressed, and isolating Vitamin C from the cell or the culture medium.