Patent ID: 8426172
Filing Date: 2013-04-23
Classification: C12N,C12P

Abstract:
1. A transformant capable of producing isopropanol, which is constructed by transferring the following genes (a) to (d) into Escherichia coli JM 109: (a) DNA comprising the base sequence of SEQ ID NO: 13, or a DNA having 95% or more sequence identity to SEQ ID NO: 13 and encoding an enzyme having acetyl-CoA acetyltransferase activity; (b) DNA comprising the base sequence of SEQ ID NO: 14, or a DNA having 95% or more sequence identity to SEQ ID NO: 14 and encoding an enzyme having acetoacetyl CoA:acetate CoA-transferase activity; (c) DNA comprising the base sequence of SEQ ID NO: 15, or a DNA having 95% or more sequence identity to SEQ ID NO: 15 and encoding an enzyme having acetoacetate decarboxylase activity; and (d) DNA comprising the base sequence of SEQ ID NO: 16, or a DNA having 95% or more sequence identity to SEQ ID NO: 16 and encoding an enzyme having isopropanol dehydrogenase activity, wherein the DNAs of (a), (b) and (c) are derived from