Patent ID: 6030794
Filing Date: 2000-02-29
Classification: A01K,C07K,C12N

Abstract:
A method of identifying the subtype or isoform of a receptor bound by an agent, said method comprising the steps of:(a) incubating said agent with one or more cell lines homozygous for a disruption in one or more of its receptor genes selected from RAR genes and RXR genes such that said disruption in said cell line(s) prevents said gene from producing an active receptor selected from the group consisting of all isoforms of RAR.alpha., all isoforms of RAR.gamma., isoform RAR.gamma.2, isoform RAR.beta.2 and all isoforms of a RXR family receptor;(b) determining the amount of agent bound by said cell line(s); and(c) comparing said amount to the amount of said agent bound by cell line(s) without said disruption.