Patent ID: 8980559
Filing Date: 2015-03-17
Classification: A61P,C12Q,G01N

Abstract:
1. A method for assessing a cancerous state of a human specimen from the pancreas, which comprises first treating specimen-derived DNA with bisulfite, second amplifying the bisulfite-treated DNA with primers which can amplify a DNA having a nucleotide sequence containing a cytosine to be analyzed and which do not contain the cytosine to be analyzed, third analyzing a nucleotide sequence of the resulting amplification product, fourth measuring a methylation frequency of the cytosine to be analyzed and detecting a methylation frequency that is higher as compared with a healthy control, and fifth determining that malignancy is present in the specimen, wherein the cytosine to be analyzed is a cytosine present in a promoter region of the Neurofilament3 gene contained in the specimen and is selected from the group consisting of cytosines represented by nucleotide numbers 428, 432, 443, 451, 471, 475, 482, 491, 499, 503, 506, 514, 519, 532, 541, 544, 546, 563, 566, 572, and 580 in the nucleotide sequence of SEQ ID No: 1.