Patent ID: 6060309
Filing Date: 2000-05-09
Classification: A61K,C12N

Abstract:
A method for preparing a CD56 negative, CD8 negative responder cell clone that proliferates when combined with a selected antigenic peptide presented by a stimulator cell, comprising:isolating CD56 negative, CD8 negative cells comprising the steps of:plating peripheral blood mononuclear cells to separate adherent cells from non-adherent cells,passing said non-adherent cells over nylon wool, wherein the flow-through is enriched for T cells,incubating said flow-through enriched for T cells with antibodies to CD56 and CD8 to remove CD56 positive, CD8 positive cells,thereby forming CD56 negative, CD8 negative responder cells;stimulating the CD56 negative, CD8 negative responder cells with pulsed or primed stimulator cells;restimulating the stimulated CD56 negative, CD8 negative responder cells with pulsed or primed stimulator cells; andisolating a CD56 negative, CD8 negative responder cell clone that proliferates when combined with a selected antigenic peptide presented by a stimulator cell.