Patent ID: 8993486
Filing Date: 2015-03-31
Classification: B01J,C40B,G01N

Abstract:
1. A method of identifying agents that exhibit synergy in an assay, said method comprising a) performing an orthogonally pooled screening assay on separate pooled mixtures of agents using said screening assay to measure the activity of the pooled mixtures, wherein every agent is present in at least two separate pooled mixtures in said orthogonal pooled screening assay; b) isolating an orphan active pooled mixture detected in step a), wherein said orphan active pooled mixture exhibits a threshold activity, and wherein all other pooled mixtures containing an agent from said orphan active pooled mixture do not exhibit said threshold activity; c) verifying said threshold activity of the orphan active pooled mixture of step b) by determining that the orphan active pooled mixture comprises agents for which the summation of the activities of the other pooled mixtures containing the individual agents not exhibiting said threshold activity of said orphan active pooled mixture is less than the level of said threshold activity of said orphan active pooled mixture isolated in step b) which exhibits said threshold activity, thereby identifying a verified orphan active pooled mixture; and d) testing individual agents and binary mixtures from the separated mixtures of agents comprising every possible pair of agents from all agents within the verified isolated orphan active pooled mixture having a threshold activity identified in step c); wherein the agents of step d) of a binary mixture exhibit synergy when the binary mixture exhibits greater activity in step d) than the sum of the individual activities of the agents in the orphan active pool not exhibiting said threshold activity in step c), wherein the activity of said orphan active pool is not accounted for by the additive activities of singly-active compounds.