Patent ID: 7504230
Filing Date: 2009-03-17
Classification: A61P,G01N,Y10T

Abstract:
1. A method to identify a set of signal transduction proteins whose intracellular localization pattern changes in response to toxic compounds, which method comprises identifying a first set of signal transduction proteins; providing a set of toxic compounds; contacting each signal transduction protein of the first set with each one of the toxic compounds; determining the changes in intracellular localization pattern of each of the signal transduction proteins of said first set in response to each of the toxic compounds; discarding those signal transduction proteins from said first set whose changes in intracellular localization pattern are redundant; adding new signal transduction proteins to provide a second set of signal transduction proteins; contacting each signal transduction protein of the second set with each of the toxic compounds; determining the changes in the intracellular localization pattern of each of the signal transduction proteins of said second set in response to each of the toxic compounds; discarding those signal transduction proteins from said second set whose changes in intracellular localization patterns are redundant; adding new signal transduction proteins to provide a third set of signal transduction proteins; and repeating the steps for which the second set of signal transduction proteins was used until a final set of proteins is identified which provides at least five principal components with respect to the range of compounds marketed as small organic molecules.