Patent ID: 8263414
Filing Date: 2012-09-11
Classification: B01J,B01L,G01N,Y10T

Abstract:
1. An apparatus for dispensing a uniform layer of droplets from a sample of a biological fluid from at least 1 mm above a surface containing reagents for reacting with said reagents comprising: (a) a nozzle for dispensing said sample of a biological fluid, said nozzle having a closed channel ending in one or more openings having diameters greater than 100 μm and up to about 1000 μm for dispensing under pressure said sample of biological fluid as droplets having diameters in the range of 0.1 to 2 mm onto a predetermined region of said reagent-containing surface; (b) a reservoir for supplying said biological sample to said nozzle of (a); (c) automated means for positioning said nozzle with respect to said reagent-containing surface or said surface with respect to said nozzle such that said nozzle is positioned at least 1 mm above said reagent-containing surface during dispense such that the droplets fall onto the reagent-containing surface; (c) means for dispensing under pressure said biological sample via said nozzle openings onto predetermined portions of said reagent-containing surface; and (d) means for reading the results of the reaction between said biological fluid and said reagents from a spectrographic image of the reagent-containing surface.