Patent ID: 8703815
Filing Date: 2014-04-22
Classification: A61K,A61P,C07C,C09B,G01N

Abstract:
1. A method for assaying for the presence of transthyretin (TTR) in a sample to be assayed that comprises the steps of: a) admixing a binding/reaction sufficient amount of a covalent TTR kinetic stabilizer compound of Formula II with a sample to be assayed, wherein the depicted structure Ar is a hydrocarbyl aromatic ring structure, R R n is one; L is a linker that is HC═CH; X is a reactive substituent that reacts with an amine in an aqueous environment to bond the amine to the depicted compound that is a carboxylic ester or thioester, an azetidin-2-one, a Michael acceptor, an epoxide, aziridine or thienyl group or a □-haloacetyl group, and L and X are in a 1,3-substitution relationship on the ring to which they are bonded; and Z is H, or a second linker, L b) maintaining that admixture for a time period sufficient for said compound to bind to TTR present in the sample, react therewith and form a covalently-bound reacted compound, and c) determining the presence of said reacted compound.