Patent ID: 6015880
Filing Date: 2000-01-18
Classification: B01J,B82Y,C07H,C07K,C40B

Abstract:
A method of step-by-step synthesis of an array of different chemical compounds at microdrop-sized loci, where each compound is covalently attached to or beneath the surface of an undifferentiated substrate comprising the steps of:(a) applying through a single jet of a multiple jet reagent dispenser at least one microdrop of a first reagent in liquid form to a loci on said surface, wherein said undifferentiated substrate is a porous material comprising a sheet having internal straight, essentially parallel pores, and is chemically prepared to react with said first reagent to covalently attach said reagent to said undifferentiated substrate;(b) displacing said multiple jet reagent dispenser relative to said surface, or the surface with respect to said multiple jet reagent dispenser, and applying at least one microdrop containing either the first reagent or a second reagent from a different dispenser jet to a second loci on said surface wherein said substrate is chemically prepared to react with said reagent to covalently attach said reagent to said substrate;(c) optionally repeating step (b) at least one time using either said first reagent, said second reagent, or different reagents in liquid form from different dispenser jets wherein each of said reagents covalently attaches to said substrate to form covalently attached compounds;(d) washing said substrate to remove unattached reagents;(e) modifying said attached reagents;(f) repeating steps (a) through (e) with the same or different reagents at various loci on the substrate until the synthesis of different chemical compounds at each of at least 20 said loci is completed; and(g) optionally removing the attached compounds at said loci from said substrate, wherein said optional removing is performed in a selective or a non-selective manner.