Patent ID: 8399252
Filing Date: 2013-03-19
Classification: C12M,C12N

Abstract:
1. A method of releasing cells, comprising: a) providing cultured cells on a cell culture support, wherein the cell culture support comprises a multi-layer polyelectrolyte coating immobilized on a substrate, wherein the multi layer polyelectrolyte coating comprises at least one layer of poly-(di(ethyleneglycol)methylether methacrylate)-co-polyacrylic acid and b) releasing the cultured cells from the cell culture support by adding a releasing solution comprising DMSO, wherein the cells are selected from the group consisting of embryonic stem cells, adult stem cells, induced pluripotent stem cells, dendritic cells, hematopoietic cells, mesenchymal stromal cells, neural cells, reprogrammed cells and de-differentiated cells.