Patent ID: 7723105
Filing Date: 2010-05-25
Classification: A61K,C12N

Abstract:
1. A method for growing, expanding, and/or maintaining isolated mammalian cells in vitro, the method comprising: (a) conditioning a culture medium with c-Met Transgenic Murine Hepatocyte (MMH) cells; (b) removing or separating the MMH cells therefrom to provide an MMH cell-free conditioned cultured medium; (c) contacting one or more of the isolated mammalian cells with the conditioned culture medium obtained from step (b); and (d) culturing the mammalian cells in the conditioned culture medium for a period of time sufficient to grow, expand, and/or maintain the mammalian cells in the contacted culture medium, wherein the cultured mammalian cells are selected from the group consisting of bone marrow cells, cord-blood stem cells, non-human embrional stem cells, adult stem cells, endodermal cells, ectodermal cells, mesodermal cells, natural killer (NK) cells, dendritic cells, and endothelial cells.