Patent ID: 6245884
Filing Date: 2001-06-12
Classification: C12N,C12Q,G01N

Abstract:
A method of selecting an agent that alters the cleavage of an amyloid precursor protein (APP) substrate by a secretase, comprising the steps of:a) obtaining substantially pure vesicles;b) permeabilizing the substantially pure vesicles;c) incubating the permeabilized vesicles with the APP substrate in a first incubation solution;d) detecting the cleavage of the APP substrate in the first incubation solution, wherein the amount of cleavage is proportional to the proteolytic activity of the secretase;e) incubating the agent, the permeabilized vesicles, and the APP substrate in a second incubation solution;f) detecting the cleavage of the APP substrate in second incubation solution, wherein the amount of cleavage is proportional to the proteolytic activity of the secretase;g) comparing the cleavage of the APP substrate in the first and second incubation solutions; andh) the agent is thereby selected that alters the cleavage of the APP substrate of the second incubation solution from that in the first incubation solution.