Patent ID: 6632438
Filing Date: 2003-10-14
Classification: A61K,C12N

Abstract:
An insertion plasmid comprising at least one DNA sequence coding for a HCMV protein, a promoter located upstream of the DNA sequence which is capable of inducing expression of the HCMV protein in a poxvirus, and flanking DNA sequences; wherein the poxvirus is selected from a group consisting of:(i) recombinant vaccinia virus wherein regions C7L-K1L, J2R, B13R+B14R, A26L, A56R and I4L have been deleted therefrom, or wherein the open reading frames for the thymidine kinase gene, the hemorrhagic regions, the A type inclusion body region, the hemegglutinin gene, the host range gene region, and the large subunit, ribonucleotide reductase have been deleted therefrom; (ii) NYVAC vaccinia virus; (iii) ALVAC canarypox virus; and (iv) attenuated canarypox virus wherein said canarypox virus is attenuated through more than 200 serial passages on chick embryo fibroblasts, a master seed therefrom was subjected to four successive plaque purifications under agar, from which a plaque clone was amplified through five additional passages.