Patent ID: 7816106
Filing Date: 2010-10-19
Classification: C12P,Y10S

Abstract:
1. A method of producing an amide from the corresponding nitrile comprising the following steps, i) providing a microorganism capable of producing a nitrile hydratase biocatalyst, ii) culturing the microorganism in a growth medium , iii) storing the microorganism in a storage medium that comprises water and the growth medium in step ii), iv) contacting the nitrile with the microorganism in the storage medium of step iii) without recovery of the microorganism using centrifugation or filtration and thereby converting the nitrile to the amide, wherein the microorganism is stored in step iii) as a non-actively growing culture in the storage medium and the term non-actively growing culture means that the metabolism in the microorganism cells is substantially zero and the microorganism is Rhodococcus rhodochrous NCIMB 41164.