Patent ID: 6489129
Filing Date: 2002-12-03
Classification: G01N,Y10S

Abstract:
A method for the determination of an antigen-specific antibody of the immunoglobulin M class in a biological sample comprising an interfering antibody of the immunoglobulin G class having the same antigen specificity as said antibody of the M class, said method comprising the steps of:a. incubating said sample in a test mixture comprising: 1) two different receptors, R1 and R2, wherein each of the said receptor binds specifically to said antibody of the M class, R1 is bound directly or indirectly to a solid phase, R1 is a binding partner in polymeric form, and R2 carries a label, and 2) a binding partner in a monomeric form that binds specifically to said antibody of the M class and to the antigen binding site of the antibody of the G class in an amount sufficient to reduce interference from said antibody of the G class, b. allowing a complex comprising R1, said antibody of the M class, and labeled R2 to form, and c. measuring said label bound by R2 to said antibody of the M class as a measure of said antibody of the M class present in said sample.