Patent ID: 8993229
Filing Date: 2015-03-31
Classification: C12Q

Abstract:
1. A method for extracting and distinguishing infectious norovirus from inactivated norovirus virions comprising: (a) obtaining a biological sample suspected of containing infectious norovirus and inactivated norovirus virions, (b) mixing a first quantity of said biological sample with a solution containing a solid support conjugated with a glycoprotein moiety capable of binding said infectious norovirus to form a first solution, (c) incubating said first solution for a time period effective to allow binding of said infectious norovirus to said solid support conjugated with a glycoprotein moiety capable of binding said infectious norovirus to form a solid substrate bound with infectious norovirus, wherein said inactivated norovirus virions do not bind to said glycoprotein moiety, (d) separating said solid substrate bound with infectious norovirus and said biological sample suspected of containing infectious norovirus and inactivated norovirus virions, (e) eluting said infectious norovirus RNA from said infectious norovirus bound to said solid substrate to form an infectious norovirus RNA containing solution, (f) performing RT-PCR on said infectious norovirus RNA containing solution to obtain a first Ct value, (g) separating all norovirus RNA from a second quantity of said biological sample to form a total norovirus RNA solution, (h) performing RT-PCR on said total norovirus RNA solution to obtain a second Ct value, and (i) comparing said first Ct value and said second Ct value to distinguish between said infectious norovirus and said inactivated norovirus virions in said biological sample.