Patent ID: 6720164
Filing Date: 2004-04-13
Classification: C12Q

Abstract:
A method of quantifying a substrate in a sample which method comprises the steps of:(a) contacting a sample containing a substrate with a reaction reagent comprising at least a dehydrogenase, a coenzyme, an electron mediator and a (2-(4-indophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2-tetrazolium performing an enzyme reaction and a redox reaction between which produces a water-soluble, stable formazan, then, (b) detecting the water-soluble formazan formed from the (2-(4-indophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium as the final reaction product using an electrode system made of electrically conductive materials, and (c) correlating an increase in electrical current in the electrode system with a quantity of substrate in the sample.