Patent ID: 8614077
Filing Date: 2013-12-24
Classification: C07C,C12C,C12M,C12P,Y02E,Y10T

Abstract:
1. A method for recovering isobutanol from a fermentation medium, comprising: (a) culturing a genetically engineered microorganism in a fermentation medium comprising water and a feedstock comprising isobutanol, said genetically engineered microorganism, and a fermentable carbon source; (b) extracting the isobutanol in a portion of the fermentation medium with a water immiscible, isobutanol-selective liquid extractant, thereby forming an isobutanol-rich liquid phase and a water-rich liquid phase; (c) separating the isobutanol-rich liquid phase and a water-rich liquid phase; (d) recovering the isobutanol from the isobutanol-rich liquid phase; wherein: said genetically engineered microorganism is engineered to express or over-express a metabolic pathway that converts pyruvate to isobutanol; and said metabolic pathway comprises an acetohydroxy acid synthase (ALS) enzyme; and said genetically engineered microorganism includes a deletion of pyruvate decarboxylase.