Patent ID: 6993958
Filing Date: 2006-02-07
Classification: B01J,G01N

Abstract:
1. A microfluidic circuit for introducing or removing a fluid sample comprising: a separation element; at least three substrate layers bonded together and with two adjacent substrate layers including at least one set of through holes in each of the layers being coaxial; wherein a first through hole extends through the outermost layer and has a diameter that is the same as the outer diameter of a discrete capillary tube adapted to transport the fluid sample to or from the separation element; wherein a second through hole extends through the layer adjacent the outermost layer and is coaxially aligned with the first through hole and is in fluid communication with the separation element, the diameter of the second through hole being sufficiently smaller than the first through hole to provide a surface against which the capillary tube may be sealed; and wherein the first and second holes are aligned with respect to one another by two mechanical alignment holes formed through each substrate layer.