Patent ID: 6541223
Filing Date: 2003-04-01
Classification: C07K,C12N

Abstract:
A method for the production of a viral entity selected from the group consisting of a virus, a viral antigen and a viral product, said method comprising the steps of:inserting a DNA expression vector coding for a stress protein into a eucaryotic cell line that is non-permissive for said virus; selecting from said eucaryotic cell line at least one modified eucaryotic cell line, wherein said at least one modified eucaryotic cell line is permissive for said virus; introducing an infective material to said at least one modified eucaryotic cell line to produce at least one infected modified eucaryotic cell line; incubating said at least one infected modified eucaryotic cell line; and harvesting at least one resultant viral entity from the group consisting of a virus, a viral antigen and a viral product from said at least one infected modified eucaryotic cell line.