Patent ID: 6576465
Filing Date: 2003-06-10
Classification: A61K,C12N

Abstract:
A method for isolating a bone osteogenic accessory cell comprising the of:(a) prociding a starting cell population; (b) subjecting said population to density isolation by equilibrium density centrifugation to obtain a low density cell fraction wherein the low density is about between 1.050 gm/cm3 and about 1.090 gm/cm3; (c) subjecting said low density cell fraction to immune adherence isolation based on TDF&bgr;II receptor expression and recovering an immune adherent cell; and (d) subjecting the immune adherent cell of step (c) to isolation based on low cellular complexity determined by flow cytometric analysis, whereby a low complexity cell isolated in step (d) is a vone osteogenic accessory cell.