Patent ID: 6066459
Filing Date: 2000-05-23
Classification: C12Q,G01J,G01N,G06K

Abstract:
A method of hybridization based multicolor chromosome banding comprising the steps of:(a) providing a metaphase chromosome spread of a first species and chromosome paints of a second species, at least some of said chromosome paints being labeled with a different fluorophore or combination of fluorophores;(b) hybridizing said chromosome paints with said metaphase chromosome spread;(c) using a fluorescence microscope being optically coupled to a multiband collection device for collecting spectral data from said chromosome spread; and(d) based on said collected data calculating a color image in which pixels associated with each of said different fluorophores or combination of fluorophores are attributed distinctive colors, thereby a banded color image of said metaphase chromosome spread is obtainable;wherein said method is for an application selected from the group consisting of providing a color banding karyotype of embryonic cells, providing a color banding karotype of white blood cells, providing a color banding karyotype of malignant cells, providing a color banding karyotype of cells examined for malignancy, detecting the stage of a malignancy, following the efficiency of an anti-cancer treatment and detecting chromosomal aberrations following exposure to mitogenic agents.