Patent ID: 8871527
Filing Date: 2014-10-28
Classification: G01N,Y10S

Abstract:
1. A method for determining the presence of at least one hormone form of an endogenous hormone that is involved in diabetes or metabolic syndrome in a sample comprising: (a) forming within a free flow electrophoretic (FFE) chamber a separation zone between an anode and a cathode that comprises a zone I formed by at least one separation buffer medium type I (SBM type I) and a zone II formed by at least one separation buffer medium type II (SBM type II), wherein the SBM type I yields denaturing conditions and comprises at least one additive at an effective concentration and the SBM type II yields non-denaturing conditions and does not contain the additive at the effective concentration; (b) introducing the sample into the separation zone; and (c) transferring the at least one hormone form from the SBM type I into the SBM type II, or transferring the at least one hormone form from the SBM type II into the SBM type I during the FFE separation, thereby separating the hormone form from an interfering form that would interfere with measuring a parameter of the hormone form by an analytical method employed in step (d), wherein the endogenous hormone is selected from the group consisting of insulin, glucagon-like-peptide 1 (GLP-1), ghrelin, cholecystokinin (CKK), leptin, and obestatin; and (d) measuring the parameter of the hormone form by the analytical method.