Patent ID: 8008074
Filing Date: 2011-08-30
Classification: A61P,G01N

Abstract:
1. A method of identifying a compound, a parathyroid hormone (PTH) ligand, or a fragment of a PTH ligand that improves bone mass, comprising: a) contacting an in vitro cell expressing a PTH receptor with a test compound, a PTH ligand, or a fragment of a PTH ligand, wherein the PTH receptor is selected from the group consisting of low density lipoprotein related protein (LRP) 5/6, transforming growth factor beta receptor, type II (TGFβRII), bone morphogenic protein receptor, type II (BMPRII), activin A receptor, type IIA (ActRII), and activin A receptor, type IIB (ActRIIB); and b) determining whether an increase in binding between the PTH receptor and parathyroid hormone 1 receptor (PTH1R) occurs in the cell contacted with the compound, the PTH ligand, or the fragment of a PTH ligand, as compared to a control, said increase being an indication that the compound, PTH ligand, or fragment of PTH ligand improves bone mass.