Patent ID: 6130092
Filing Date: 2000-10-10
Classification: C07K,C12N

Abstract:
A ribozyme library comprising a collection of ribozyme genes encoding a hammerhead structure and flanking sequences of random nucleotides cloned at least once into an expression cassette for ribozyme expression, wherein said expression cassette contains a T7 promoter proximal to the 5' end of said cassette, an adenoviral va-RNA-gene adjacent to said promoter, and a loop region located in the central part of said gene, said loop region defined as a series of adjacent nucleotides between a first nucleotide and a second nucleotide, said first nucleotide further linked on either side to adjacent nucleotides other than the second nucleotide, and the second nucleotide further linked on either side to adjacent nucleotides other than the first nucleotide.