Patent ID: 6077662
Filing Date: 2000-06-20
Classification: A61K,C07K,C12N

Abstract:
A method of making a retrovirus-like particle, said method comprising the steps of:(a) operably linking a coding sequence for a truncated envelope protein of a retrovirus to a baculovirus early promoter, late promoter, or hybrid late/very late promoter, and inserting into baculovirus vector to form an envelope protein expression construct;(b) operably linking a protease-encoding sequence to regulatory sequences in a vector for expression in insect cells to form a protease expression construct;(c) simultaneously introducing into an insect cell the envelope protein expression construct of step (a) and the protease expression construct of step (b) and allowing for expression of the truncated retrovirus envelope protein and the protease,whereby retrovirus-like particles are formed by the insect cells of step (c).