Patent ID: 8481281
Filing Date: 2013-07-09
Classification: C12Q

Abstract:
1. A method for determining the resistance of a microorganism to a drug by detecting growth of the microorganism in the presence of the drug, comprising: (a) incubating the microorganism in (b) applying a sample comprising the biomarker molecules of(a)(i) and applying a sample comprising the biomarker molecules of (a)(ii) to a mass spectrometry system and producing ion mass fragments of the biomarker molecules of (a)(i) and (a)(ii); (c) analyzing the ion mass fragments of tile biomarker molecules of (a)(ii) and using such analysis to determine a predicted mass for the one or more biomarker molecules of a(i) using an algorithm that determines a predicted mass based on the incorporation of the isotopic label into the one or more biomarker molecules of (a)(ii); (d) comparing the predicted mass of (c) with an observed mass of the one or more biomarker molecules of the microorganism growing in the isotopically-labeled medium with drug of (a)(i) using an algorithm based on which a probability that the biomarker molecules in the two media match can be determined, thereby indicating if growth of the microrganism has occurred in the isotopically-labeled medium with drug if the biomarkers have a high probability of matching; and (e) determining the microorganism to be resistant to the drug if the biomarkers have a high probability of matching.