Patent ID: 6153441
Filing Date: 2000-11-28
Classification: G01N

Abstract:
A method for identifying an agonist or antagonist of the interaction between a human CC Chemokine Receptor 7 (CCR7) polypeptide and a human Chemokine Beta-9 (CK.beta.-9) polypeptide, wherein the CCR7 polypeptide has an amino acid sequence comprising SEQ ID NO: 1 and the CK.beta.-9 polypeptide has an amino acid sequence comprising SEQ ID NO: 2, said method comprising:(a) in the presence of labeled or unlabeled said human CK.beta.-9 polypeptide, contacting a cell expressing on the surface thereof said human CCR7 polypeptide, said human CCR7 polypeptide being associated with a second component capable of providing a detectable signal in response to the binding of said human CK.beta.-9 polypeptide to said human CCR7 polypeptide, with a compound; and(b) determining whether the compound activates or inhibits said detectable signal, wherein a compound that activates said signal is an agonist and a compound that inhibits said signal is an antagonist.