Patent ID: 6548286
Filing Date: 2003-04-15
Classification: A61K,C07K,C12N

Abstract:
A method for producing a stock of recombinant adeno-associated virus (AAV) comprising:(a) co-transfecting cells permissive for adeno-associated virus replication with (i) a recombinant adeno-associated virus vector which contains a foreign DNA sequence and which can be incorporated into an infectious AAV virion; and (ii) a recombinant helper virus DNA which provides viral functions sufficient for replication and packaging into infectious virions of said recombinant adeno-associated virus vector, wherein an AAV REP 78 or REP 68 protein is expressed under regulatory control of an AAV promoter, and wherein a decreased level of REP 78 or REP 68 protein is expressed relative to the level of REP 78 or REP 68 protein expressed by a host cell that expresses wild-type REP 78 or REP 68 protein under regulatory control of an AAV REP 78 or REP 68 promoter; and (b) collecting virions produced.