Patent ID: 6502039
Filing Date: 2002-12-31
Classification: G16B

Abstract:
A method for calculating indicia of differences in gene-expression level in a plurality of array hybridizations, the method comprising:(a) determining an indication of experimental noise associated with intensity of hybridization signal for a gene on each array hybridization; (b) using the determined experimental noise indication to determine a first analytical probability distribution function describing distribution values of intensity for the gene on each array hybridization, assuming the noise is Gaussian and by applying Bayes Theorem; (c) using the first analytic probability distribution functions to derive a second analytical probability distribution function describing an indication of a gene-expression difference of a differentially-expressed gene; and (d) applying the second probability distribution function of the differentially-expressed gene using experimentally-derived intensities and noise values from the array hybridizations to determine values related to the gene-expression difference.