Patent ID: 6221576
Filing Date: 2001-04-24
Classification: A61K,C12N,Y10S

Abstract:
A process for preparing a final composition containing cells derived from mononuclear cells, the cells of the final composition being at least one of (i) macrophages, (ii) activated macrophages, (iii) monocyte-derived antigen-presenting cells (MD-APCs) capable of presenting specific antigens and inducing a specific immune response, and (iv) precursors of said macrophages, activated macrophages, and MD-APCs; said process comprising performing a selective enrichment of human blood to obtain an initial composition enriched in mononuclear cells relative to said blood and containing monocytes, and performing mononuclear cell culture on said initial composition to obtain said final composition, wherein after said selective enrichment and before mononuclear cell culture said initial composition is not subjected to centrifugation to eliminate residual red blood cells and granulocytes, said mononuclear cell culture occurring in the presence of red blood cells and granulocytes.