Patent ID: 8728979
Filing Date: 2014-05-20
Classification: C12Q,G01N

Abstract:
1. A method for identifying cells comprising the following steps: (a) generating loess-smoothed timing ratio values for a replication timing test profile for a population of cells; (b) comparing the loess-smoothed replication timing ratio values for the replication timing test profile to loess-smoothed replication timing ratio values for replication timing reference profiles for a variety of cell types, which are contained in a database; (c) based on the comparison of step (b), determining that the replication timing test profile is substantially the same as a replication timing reference profile of a cell type if (1) at least about 95% of the loess-smoothed replication timing ratio values for the replication timing test profile and the replication timing reference profile for said cell type differ by less than about 0.5; or (2) less than about 5% of the loess-smoothed replication timing ratio values for the replication timing test profile and the replication timing reference profile for said cell type differ by more than about 0.5; (d) identifying the cell type of the population of cells as the cell type of the replication timing reference profile if the replication timing test profile is determined to be substantially the same as the replication timing reference profile for said cell type; and (e) displaying the cell type identified in step (d) to a user; wherein each loess-smoothed replication timing ratio value of the loess-smoothed replication timing ratio values is equal to log wherein the replication timing test profile for the population of cells is generated by hybridizing fluorescently labeled DNA from a population of cells to a genomic array having an average probe spacing of about 6 kb or less; and wherein steps (a), (b), (c), (d) and (e) are performed by a computer configured to perform steps (a), (b), (c), (d) and (e).