Patent ID: 9116088
Filing Date: 2015-08-25
Classification: C12N,G01N

Abstract:
1. A method of isolating DNA from cells in a sample comprising the steps of: (a) selectively lysing the cellular membranes of the cells in the sample without lysing the nuclear membranes of the cells to produce intact nuclei from the cells; (b) separating the intact nuclei from the sample by a nuclei size exclusion barrier in a nuclei separation region of a microfluidic device by flowing the sample through the nuclei size exclusion barrier in a first direction; (c) flowing an elution buffer in a second direction into the nuclei separation region of the microfluidic device to resuspend the isolated nuclei; wherein the first direction is substantially orthogonal to the second direction; (d) delivering the resuspended isolated nuclei to a nuclei lysis region of the microfluidic device; and (e) lysing the resuspended isolated nuclei in the nuclei lysis region of the microfluidic device to release the isolated DNA.