Patent ID: 7633620
Filing Date: 2009-12-15
Classification: B01L,G01N,G02B

Abstract:
1. A method for detecting laser-induced epifluorescence, comprising: fixing a sample whose surface is labeled with fluorescent material at a first focal point of an elliptical or spherical reflecting mirror; passing light emitted from a laser through an exciter filter and then through a hole of the elliptical or spherical reflecting mirror that is disposed between the exciter filter and the sample control means and focusing the filtered light to a surface of the sample positioned at the first focal point of the elliptical or spherical reflecting mirror with a proper size; focusing scattered incident light and fluorescence emitted from the sample positioned at the first focal point of the elliptical or spherical reflecting mirror to a second focal point of the elliptical or spherical reflecting mirror by reflection of the reflecting mirror; removing noise from the scattered incident light and the fluorescence reflected by the elliptical or spherical reflecting mirror through a spatial filter positioned at the second focal point; converting the noise-removed light into parallel light by a collimator; filtering the parallel light through a fluorescent filter to remove the scattered incident light; and providing a pure fluorescence component to an optical detector.