Patent ID: 7960145
Filing Date: 2011-06-14
Classification: C07K

Abstract:
1. A method for producing species-specific antibodies comprising co-culturing B cells and monocytes in the presence of a denatured antigen, generating activated B cells by adding a stimulation media comprising CD40 ligand (CD40L), or an anti-CD40 antibody and one of the following cytokine stimuli: (a) interleukin-2(IL-2) and interleukin-21(IL-21); (b) interleukin-5(IL-5), interleukin-10(IL-10), and transforming growth factor-beta (TGF-β; or (c) interleukin-4(IL-4), interleukin-13(IL-13), or both IL-4 and IL-13; detecting the production of a species-specific antibody; and generating a hybridoma from the activated B-cells.