Patent ID: 8709823
Filing Date: 2014-04-29
Classification: G01N

Abstract:
1. A method for performing a homogeneous biological assay comprising: preparing at least one reaction volume and a matrix volume wherein said reaction volume and said matrix volume each comprise a biological fluid; adding an acceptor reagent to said reaction volume; adding a lanthanide donor reagent to said reaction volume; adding a lanthanide donor reagent to said matrix volume; applying an optical excitation to said reaction volume; obtaining a first integrated intensity value for the luminescent intensity of said reaction volume at a single characteristic emission wavelength of said acceptor reagent; applying an optical excitation to said matrix volume; obtaining a second integrated intensity value for the luminescent intensity of said matrix volume at said single characteristic emission wavelength of said acceptor reagent, wherein said luminescent intensity of said matrix volume comprises luminescent emission from said lanthanide donor reagent at said single characteristic emission wavelength of said acceptor reagent; and correcting said first integrated intensity value by applying a mathematical expression to said first integrated intensity wherein said mathematical expression comprises said second integrated intensity value.