Patent ID: 6190895
Filing Date: 2001-02-20
Classification: C12N

Abstract:
An isolated polynucleotide fragment selected from the group consisting of:(a) an isolated polynucleotide fragment encoding a 1-deoxyxylulose-5-phosphate synthase obtained from Mentha piperita;(b) an isolated polynucleotide fragment encoding the 1-deoxyxylulose-5-phosphate synthase set forth in SEQ ID NO:4, 6 or 8;(c) an isolated polynucleotide fragment encoding a polypeptide, wherein said encoded polypeptide has 1-deoxyxylulose-5-phosphate synthase activity, and wherein said polynucleotide hybridizes to the complementary strand of the polynucleotide set forth in SEQ ID NO:3, 5, or 7 under the following set of stringent hybridization conditions:ten hours hybridization in 42.degree. C. buffer consisting of 5.times. Denhardt's solution, 0.1% sodium dodecyl sulfate, and 5.times. SSPE;and said polynucleotide remains hybridized to the polynucleotide set forth in SEQ ID NO:3, 5 or 7 under the following set of stringent wash conditions:two 15 minute washes in 2.times. SSC at room temperature followed by two 20 minute welshes in 0.2.times. SSC at 65.degree. C.