Patent ID: 6165736
Filing Date: 2000-12-26
Classification: C07K,G01N

Abstract:
A method for developing a diagnostic procedure for detecting a stage of bacterial infection of an animal by an infecting bacterial pathogen comprising the steps of:(A) producing an animal population antibody profile specific for said infecting bacterial pathogen from animals having various stages of infection from said infecting bacterial pathogen wherein said profile contains a plurality of types antibody in response to said infecting bacterial pathogen;(B) hyper-immunizing a lab animal with said infection bacterial pathogen so that said lab animal produces serum that has a lab animal antibody profile that substantially matches said animal population antibody profile;(C) cloning bacteria using fragments of nucleic acid from said infecting bacterial pathogen to produce a plurality of cloned bacteria groups, each cloned bacterial group having been produced using a different fragment of nucleic acid;(D) culturing each cloned bacteria group separately;(E) denaturing and separating proteins from each cloned bacteria group;(F) contacting said denatured proteins from each cloned bacteria group with serum from said lab animals;(G) selecting said cloned bacteria groups that produce proteins that give a positive immune response to the serum; and(H) using said proteins that give a positive immune response to said serum to diagnose a stage of infection from said infecting bacterial pathogen in an animal.