Patent ID: 6140134
Filing Date: 2000-10-31
Classification: G01N,Y10S,Y10T

Abstract:
A method of determining the concentration of an analyte in a sample, said method comprising(a) establishing an analyte gradient by the steps of(i) applying said sample to a sample application pad or chamber comprising an array of capillary channels complementary to a diluent pad or chamber;(ii) applying a diluent to a diluent application chamber or pad comprising an array of capillary channels that is complementary to said sample application pad; and(iii) bringing said sample application chamber or pad into contact with said diluent application chamber or pad;(b) bringing said gradient into operable contact with an indicator zone comprising a mobile binding member;(c) bringing said indicator zone into operable contact with a test zone comprising a fixed binding member, wherein a detectable signal that indicates the concentration of said analyte is produced, wherein said signal is either generated by a signaling substance conjugated to a mobile binding member which binds to said analyte or said fixed binding member, or said signal is generated by direct detection of said analyte in said test zone.