Patent ID: 8835120
Filing Date: 2014-09-16
Classification: G01N

Abstract:
1. An immunoassay for quantifying an amount of cardiac troponin-T (cTnT) in a test sample, the immunoassay comprising the steps of: a) contacting a test sample suspected of containing cTnT with n capture antibodies (C) that bind to at least n epitopes on cTnT to form a mixture comprising n-capture antibody:cTnT complex (C b) contacting said mixture comprising a n-capture antibody-cTnT epitope complex (C wherein n′ and n are independently an integer from 1 to 10, and antibodies C and D bind to (n +n′) different epitopes of cTnT, wherein the sum of n and n′ is greater than 2, and wherein each antibody D binds to at least one epitope on cTnT that is different than any of the epitopes to which any of the antibodies C bind.