Patent ID: 6808704
Filing Date: 2004-10-26
Classification: A01K,A61K,C12N

Abstract:
A method of testing the immune compatibility of cloned cells or tissues in a non-human mammal model, comprising:a. obtaining a donor cell from a non-human donor mammal; b. removing the nuclear DNA from a recipient oocyte, transferring the nucleus from said donor cell into the recipient oocyte to form a nuclear transfer unit, activating the nuclear transfer unit and culturing the nuclear transfer unit under conditions that result in the generation of a non-human embryo; c. isolating an embryo having at least one cell, an embryonic disc or a stem cell from said non-human embryo; d. injecting said embryo, embryonic disc, or stem cell into said non-human donor mammal at the same time as a control embryonic disc or stem cell; and e. examining the injection site for teratoma formation and signs of rejection of the injected cells, or of teratomas derived therefrom, to test the immune compatibility of the cloned cells or tissues.