Patent ID: 6294518
Filing Date: 2001-09-25
Classification: A61K,C07K

Abstract:
A method of inhibiting the motility or survival of a cancer cell, the method comprising contacting the cell with an effective amount of fusion protein having a first portion and a second portion, the first portion comprising a signal sequence capable of delivering the fusion protein into the cell and the second portion comprising a calpastatin peptide comprising the sequence Xaa-Xaa-Leu-Gly-Xaa-Xaa-Xaa-Xaa-Thr-Ile-Pro-Pro-Xaa-Tyr-Xaa-Xaa-Leu-Leu-Xa a-Xaa-Xaa-Xaa-Xaa-Xaa (SEQ ID NO:4), whereinXaa at position 1 is Glu, Asp, or Lys;Xaa at position 2 is Lys, Glu, Ala, or Asn;Xaa at position 5 is Glu, Lys, or IleXaa at position 6 is Arg, Lys, or Asp;Xaa at position 7 is Asp, or Glu;Xaa at position 8 is Asp, Val, Ser, Gly, or Glu;Xaa at position 13 is Glu, Lys, or Asp;Xaa at position 15 is Arg, Lys, or Gln;Xaa at position 16 is Glu, His, Lys, or Leu;Xaa at position 19 is Glu, Asp, Asn, Ala, or Val;Xaa at position 20 is Lys, Asp, Gln, Asn, Thr, or Met;Xaa at position 21 is Lys, Asp, Glu, Gly, or Asn;Xaa at position 22 is Thr, Glu, Gly, or Lys;Xaa at position 23 is Gly, Ala, Glu, Gln, Lys or Asp; andXaa at position 24 is Val, Ile, Asp or Gly.