Patent ID: 7826052
Filing Date: 2010-11-02
Classification: G01N

Abstract:
1. A method to correct for error in an anti-Stokes photoluminescence measurement in a Foerster-type resonance energy-transfer assay, comprising the following steps a) exciting anti-Stokes photoluminescent molecules, ions, phosphors, chelates, or particles, with one wavelength or multiple wavelengths of light wherein said wavelength or wavelengths are greater than that of an emission wavelength of acceptor molecules in said assay, b) measuring emission light signal at a wavelength, which is said emission wavelength of said acceptor molecules in said Foerster-type resonance energy-transfer assay, and which differs from the emission wavelength of said anti-Stokes photoluminescent donor, in at least two different time windows, c) correcting the emission light signal measured, comprising signals originating from non-radiative and radiative energy transfer, within the first time window by estimating the ratio of the signals from non-radiative and radiative energy transfer or the signal originating from radiative energy transfer using at least one emission light signal measured in said second time window.