Patent ID: 8119423
Filing Date: 2012-02-21
Classification: B01J,B01L,C12Q,C40B,G01N,Y10S,Y10T

Abstract:
1. A method for detecting or quantitating at least one polynucleotide sequence in a liquid sample comprising: providing a device comprising a substrate defining a sample-distribution network having (i) a sample inlet, (ii) two or more detection chambers, and (iii) a channel in fluid communication with each of the detection chambers and the sample inlet, wherein at least one of the detection chambers contains pre-loaded and dried down first contents comprising a first sequence-specific polynucleotide binding polymer for detecting or quantitating a polynucleotide sequence, and at least a different one of the detection chambers contains pre-loaded and dried down second contents comprising a second sequence-specific polynucleotide binding polymer for detecting or quantitating a polynucleotide sequence, that differs from the first sequence-specific polynucleotide binding polymer; loading a liquid sample comprising at least one polynucleotide sequence into the two or more detection chambers until the two or more detection chambers are loaded with the liquid sample such that the loading is stopped; after the two or more detection chambers are loaded with liquid sample and the loading is stopped, isolating the two or more detection chambers from one another and isolating the first contents from the second contents such that the liquid sample is prevented from flowing from one of the detection chambers to another of the detection chambers; reacting the loaded sample with at least one of the sequence-specific polynucleotide binding polymers under conditions effective to produce a detectable signal in at least one of the detection chambers; and measuring the signal produced in the detection chambers to detect or quantitate the at least one polynucleotide sequence in the sample.