Patent ID: 6153377
Filing Date: 2000-11-28
Classification: C07K,C12N

Abstract:
A method for detecting antibodies to HIV antigens in an individual, which method comprises the steps of:a) obtaining a sample of a body fluid from the individual;b) incubating said body fluid with a polypeptide selected from the group consisting of:(i) an HIV-1 transmembrane protein having the amino acid sequence MGDPMMRDNWRSELYKYKVVKIEPLGIAPTKAKRRVVQREKRADLAVGILGALFLGFLGAAGSTMGARSL TLTVQARQLLSGIVQQQNNLLRAIKDPKAQQHLLQLTVWGIKQLQARVLAVERYLKDQQLLGIWGCSGKL ICTAVPWNASWSNKSLEDIWNNMTWMQWEREINNYTNLIYSLLEESQNQQEKNEQELLQLDKWVDASLW NWSNITKWLWYIKLFIMIVGGLAGLRIVFAVLSIVNRVRQGYSPLSFQTRLPNPRGPDRPEGIDEEGGER DRDRSTRLVDISLALVWEDLRSLCLFSYHRLRDLLLIATRIVELLGRRGWEVLKYWWNLLQYVSQELKNS AVSLVNATAIAVAEGTDRVIEVVWQRAYRAIRHIHRRIRQGLERILLQVHASSLESSWQFGPG, and(ii) a fragment of the HIV-2 transmembrane protein having the amino acid sequence YSSAHGRHTRGVFVLGFLGFLATAGSAMGAASLTVSAQSRTLLAGIVQQQQQLLDVVKRQQELLRLTVWG TKNLQARVTAIEKYLQDQARLNSWGCAFRQVCHTTVPW, for a time and under conditions sufficient to form polypeptide/antibody complexes;c) incubating said body fluid/polypeptide complexes with a labeled antibody to immunoglobulin, for a time and under conditions sufficient to form polypeptide/antibody/antibody complexes; andd) detecting said label and determining therefrom the presence or absence of antibodies to HIV antigens.