Patent ID: 8340919
Filing Date: 2012-12-25
Classification: C12Q

Abstract:
1. A method for quantifying an initial concentration of a nucleic acid, comprising: amplifying a nucleic acid; producing a function representing a correlation between a fluorescence intensity which increases or decreases in proportion to an amount of the nucleic acid and an amplification cycle number or an amplification time of the nucleic acid; using the function to calculate a prior-to-amplification fluorescence intensity of the nucleic acid subtracted by a background fluorescence intensity of the nucleic acid; and calculating the initial concentration of the nucleic acid from the calculated prior-to-amplification fluorescence intensity subtracted by the background fluorescence intensity of the nucleic acid, wherein the initial concentration of the nucleic acid is calculated from the prior-to-amplification fluorescence intensity of a nucleic acid sample by using a standard calibration curve, the standard calibration curve representing a relationship between the initial concentration of the nucleic acid and the prior-to-amplification fluorescence intensity of the nucleic acid subtracted by the background fluorescence intensity of the nucleic acid.