Patent ID: 9139807
Filing Date: 2015-09-22
Classification: C12M,C12Q

Abstract:
1. A cell culture apparatus for culturing cells, comprising a cell culture substrate, a semipermeable membrane, a liquid feeding pad and a culture solution supply means, wherein the cell culture substrate comprises: the semipermeable membrane covers the culture groove and the flow grooves of the cell culture substrate; the liquid feeding pad is disposed on the substrate and forms a space over the culture groove, the first and second flow grooves and the semipermeable membrane, and the liquid feeding pad is adhered to a periphery of an area of the substrate comprising the culture groove and the first and second flow grooves; the culture solution supply means includes a liquid feeding pump and a waste liquid tank; and the cell culture apparatus is configured to continuously culture cells for 200 generations or more by continuously supplying culture solution through the first and second flow grooves to the culture groove and continuously removing culture solution and a portion of cells from the first and second ends of the culture groove through the first and second flow grooves to the waste liquid tank.