Patent ID: 9085756
Filing Date: 2015-07-21
Classification: A01N,A61K,A61P,C12N,G01N

Abstract:
1. An in vitro method of screening a differentiation factor for its effect on differentiating endoderm cells that are the in vitro progeny of isolated primate pluripotent stem (pPS) cells into pancreatic islet cells, comprising a) contacting a cell culture comprising gut endoderm cells expressing Sox17, HNF3β and HNF4α that are the in vitro progeny of isolated primate pluripotent stem (pPS) cells with a differentiation factor; b) observing a change in the gut endoderm cells from step a) associated with the differentiation of an endoderm cell expressing Sox17, HNF3β and HNF4α into an islet cell; and c) correlating the change in the gut endoderm cell with the factor.