Patent ID: 6428825
Filing Date: 2002-08-06
Classification: A61K,A61P,Y10S

Abstract:
A process for the preparation of an herbal therapeutic product for controlling blood glucose levels, extracted from pulp of fruit of Eugenia jambolana, comprising cleaning and drying the fruit to remove extraneous material from the outer layer of the fruit, deseeding the fruit to form a de-seeded pulp, soaking the de-seeded pulp in water, mixing the water and pulp to form a mixture and keeping it overnight in controlled cooled conditions to maintain activity of hypoglycemic compounds present in the fruit pulp, filtering the mixture to form a residue, washing the residue with water to extract all active compounds in the form of a clear watery solution, reducing water content of the solution to obtain a filtrate in a concentrated form, purifying the filtrate by column chromatography using slurries of different column materials to elute the mixture of hypoglycemic compounds by adding different buffers to form a second mixture, conducting thin layer chromatography of the second mixture to separate the hypoglycemic compounds in the form of bands, exposing the bands in an iodine chamber for clear demarcation of the hypoglycemic compounds and extracting the hypoglycemic compounds to recover the herbal therapeutic product containing said hypoglycemic compounds for controlling blood glucose levels.