Patent ID: 9150853
Filing Date: 2015-10-06
Classification: C12N

Abstract:
1. A method for screening a protein library for a protein having a desired activity, the method comprising: a) providing a solid surface having a plurality of distinct features, each distinct feature comprising a plurality of single-stranded anchor oligonucleotides hybridized to a plurality of double-stranded nucleic acids, wherein the plurality of double-stranded nucleic acids encode a plurality of proteins; b) hybridizing a single-stranded oligonucleotide having a first reactant to a free end of the plurality of double stranded nucleic acids; c) expressing in vitro a plurality of proteins from the plurality of double-stranded nucleic acids in the presence of a second reactant having a detectable label; and d) determining the presence of the detectable label on at least one of the distinct features, wherein the presence of the label is correlated to the presence of at least one protein that catalyzes the formation of a bond between the first and second reactants, thereby identifying at least one nucleic acid encoding the at least one protein having the desired activity.