Patent ID: 8956846
Filing Date: 2015-02-17
Classification: C12N,C12P

Abstract:
1. A method of producing cellulolytic and/or hemicellulolytic enzymes by a cellulolytic microorganism in a stirred and aerated bioreactor, comprising a growth stage in the presence of a carbon source and a production stage in the presence of a carbon-containing inductive substrate, wherein the supply of carbon-containing inductive substrate during the production stage is regulated according to the dissolved oxygen partial pressure in the growth medium, said pressure oscillating between two values Po 2min and Po 2max , the value of Po 2min being greater than the critical pressure PO 2critical below which the activity of the microorganism is affected and less than 95% of the saturation oxygen partial pressure Po 2sat set when starting the bioreactor, and the value of Po 2max being at least 5% greater than Po 2min , addition of the carbon-containing inductive substrate being started as soon as the dissolved oxygen partial pressure in the medium is greater than value Po 2max and stopped as soon as the dissolved oxygen pressure in the medium is below value Po2min.