Patent ID: 6410027
Filing Date: 2002-06-25
Classification: C07K,C12N,C40B,Y10S

Abstract:
A method of eliciting an immune response in an individual comprising administering to the individual a composition comprising an immunogenic amount of a population of complexes of heat shock protein noncovalently bound to peptides, said complexes being produced by:(a) making cDNA molecules from RNA molecules of a cancer or preneoplastic cell; (b) ligating the cDNA molecules into an expression construct such that substantially each cDNA molecule is operably associated with at least one regulatory region that controls expression of the cDNA molecule, to form a library of expression constructs; (c) introducing the library into one or more host cells; (d) culturing the host cells containing the cDNA molecules under conditions such that proteins encoded by the cDNA molecules are expressed by the host cells; and (e) recovering from the host cells complexes of heat shock protein noncovalently associated with one or more peptides.