Patent ID: 7713708
Filing Date: 2010-05-11
Classification: B01L,G01N,Y10T

Abstract:
1. An immunological assay method comprising the steps of: placing an immunological sample in a filter vessel, wherein the sample comprises an antibody containing sample selected from the group consisting of plasma and serum; adding antibody reagents to the sample; incubating the immunological sample and the reagent mixture in a filter vessel; separating the sample and reagent mixture in the filter vessel into components above and below a filter, wherein the sample mixture is separated into cellular components and liquid components; analyzing the components above or below, or both above and below, the filter in the filter vessel to determine the presence of interactions between the components; and analyzing the antigen carrier components that remain above the filter; wherein the step of analyzing the filter vessel produces unclear results, and further comprising the steps of: separating the antigen carrier components from the liquid components by capturing the antigen carrier components above the filter in the filter vessel; washing the components above the filter with a physiological salt solution; separating the antigen carrier components from the liquid components; adding antibody reagents to the washed antigen carrier components remaining above the filter in the filter vessel; incubating the antigen carrier components and the antibody reagents in the filter vessel; separating the sample and reagent mixture in the filter vessel into components above and below the filter; washing the antigen carrier components above the filter with a physiological salt solution; and analyzing the components above or below the filter in the filter vessel to determine the presence of interactions between the components.