Patent ID: 8956834
Filing Date: 2015-02-17
Classification: C12N,C12P,C12Y

Abstract:
1. A mutant acyl-ACP thioesterase gene encoding a variant of a C8-preferring acyl-ACP thioesterase comprising a mutation that changes the amino acid at the amino acid position corresponding to amino acid position 174 of SEQ ID NO:29 to valine or isoleucine; wherein the variant acyl-ACP thioesterase has a higher activity on a C8 substrate than the native acyl-ACP thioesterase while retaining the C8 acyl chain length substrate preference of the native thioesterase, and further wherein said variant comprises an amino acid sequence having at least 95% identity to amino acids 65 to 355 of SEQ ID NO:40, SEQ ID NO:43, or SEQ ID NO:45.