Patent ID: 7989170
Filing Date: 2011-08-02
Classification: C12Q

Abstract:
1. A method for detecting simultaneously bacterial species that cause zoonosis, belonging to the genera Anaplasma/Ehrlichia and Bartonella , comprising the following steps: a. placing a DNA sample under analysis in contact with a reaction mixture containing specific primers that will amplify one or more sequences from the gene msp2 and fragment 16S for b. amplifying the sequences by means of polymerase chain reaction; c. simultaneously detecting amplification products formed in the previous step by means of probes comprising SEQ ID NO: 26, SEQ ID NO: 28-30, SEQ ID NO: 32-33, and SEQ ID NO: 35-38, or their fully complementary sequences, used individually or in combination, said detection being indicative of the presence or absence of zoonosis-causing bacteria.