Patent ID: 6544507
Filing Date: 2003-04-08
Classification: A61K,A61P,C07K,C12N,Y02A

Abstract:
A viral DNA construct encoding for an adenovirus capable of replication in a human or animal tumour cell and causing death of such tumour cells comprising one or more selected transcription factor binding sites for a transcription factor the level or activity of which is increased in a human or animal tumour cell relative to that of a normal human or animal cell of the same type operatively positioned together with early viral protein gene open reading frames such as to promote expression of these open reading frames in the presence of said selected transcription factor, the protein products of those open reading frames being mechanistically directly involved in viral construct nucleic acid replication selected from the group consisting of viral polymerase, primase, nuclease, helicase, ligase, DNA terminal protein and DNA binding protein and wherein the construct encodes an adenovirus E3 promoter which has in one or more mutations to nucleotide residues which modify or delete transcription factor binding sites, as related to wild type adenovirus, selected from those in the NF1, NFKB, AP1 and ATF regions.