Patent ID: 6495318
Filing Date: 2002-12-17
Classification: C07H,C12N

Abstract:
A method of assembling nucleic acid components in a predetermined order to produce a nucleic acid multicomponent construct, comprising:(a) providing at least two nucleic acid components, each comprising at least one genetic element providing a functionality and at least one single stranded 5â€² or 3â€² terminal sequence, wherein the terminal sequence hybridizes to either a terminal sequence in another of said at least two nucleic acid components or to an oligonucleotide adaptor molecule that is supplied in addition to the at least two nucleic acid components so as to allow for specific annealing and linkage of all of the nucleic acid components in a predetermined order; (b) incubating the nucleic acid components under conditions which allow for specific annealing and assembling of the components to thereby produce the nucleic acid multicomponent construct.