Patent ID: 6136576
Filing Date: 2000-10-24
Classification: C12N,C12P,Y10S

Abstract:
A method for the production of 1,3-propanediol from a recombinant microorganism comprising the step of culturing a recombinant microorganism capable of producing 1,3 propanediol and comprising at least nucleic acid encoding a dehydratase activity and a nucleic acid encoding protein X in the presence of at least one carbon source capable of being converted to 1,3 propanediol in said recombinant microorganism and under conditions suitable for the production of 1,3 propanediol, wherein the gene encoding protein X is isolated from a glycerol dehydratase gene cluster from an organism selected from the group of genera consisting of Klebsiella and Citrobacter or wherein the gene encoding protein X is isolated from a diol dehydratase gene cluster from an organism selected from the group of genera consisting of Klebsiella Clostridium and Salmonella, wherein production of 1,3-propanediol is greater in the microorganism than in the absence of said nucleic acid encoding protein X and wherein-protein X has no enzymatic activity, and wherein the carbon source is selected from the group consisting of monosaccharides, oligosaccharides, polysaccharides, and a one carbon substrate.