Patent ID: 7892823
Filing Date: 2011-02-22
Classification: A61K,C12N

Abstract:
1. A targeting vector for homologous recombination, comprising: (a) a polynucleotide that encodes a gene-product-of-interest; b) a proximal polynucleotide having a sequence that is homologous to a proximal part of a target polynucleotide; (c) a distal polynucleotide having a sequence that is homologous to a distal part of a target polynucleotide; (d) a nuclear localization signal (“NLS”) attached to the end of either the proximal polynucleotide or the distal polynucleotide; (e) a first bait polypeptide attached to the end of either the proximal polynucleotide, wherein the NLS sequence is attached to the distal polynucleotide, or the distal polynucleotide, wherein the NLS sequence is attached to the proximal polynucleotide, wherein the first bait polypeptide binds to RAD51; and (f) a second bait peptide positioned between the proximal or distal polynucleotide, and the NLS, wherein the second bait polypeptide and the NLS comprise a combined sequence as set forth in SEQ ID NO: 8.