Patent ID: 9024008
Filing Date: 2015-05-05
Classification: C07H,C12N,C12Q

Abstract:
1. Procedure for specific isolation of total DNA content of bacterial germs of samples, in the course of which cells are lysated, the DNA content of the lysate is bound selectively to a —SiO 2 —TiO 2 -matrix of the formula SiO 2 —TiO—OH(—HCl)C 12 H 25 —O—NH, is washed and then a desalinated linear polymer nucleic acid is eluted from the matrix in an aqueous solution, characterised by that preliminarily nonviable bacterial cells are separated from viable cells on the basis of their different cell surface physical-chemical characteristics, their different cell surface permeability and ionic affinity, using a differently penetrating wash solution containing detergent and an electrolyte, and centrifugation, as a result of which exclusively double-stranded DNA deriving from the lysate of viable cells is bound on said matrix.