Patent ID: 6632673
Filing Date: 2003-10-14
Classification: C12N,C12P

Abstract:
A method for making a novel strain of S. avermitilis, comprising mutating the aveCallele in cells of a strain of S. avermitilis, which mutation results in the substitution in the aveC gene product of a different amino acid residue at one or more amino acid positions corresponding to amino acid residues 38, 48, 89, 99, 111, 136, 154, 179, 228, 238, 289 or 298 of SEQ ID NO:2, such that cells of the S. avermitilis strain in which the aveC allele has been so mutated produce a class 2:1 ratio of avermectins that is different from the ratio produced by cells of the same S. avermitilis strain that instead express only the wild-type aveC allele.