Patent ID: 8420357
Filing Date: 2013-04-16
Classification: C12N,C12Y

Abstract:
1. A method of producing a polylactate (PLA) or hydroxyalkanoate-lactate copolymer from sucrose, comprising: culturing or growing a microorganism in an environment containing lactate and sucrose or an environment containing lactate, sucrose, and hydroxyalkanoate, wherein the microorganism comprises a gene of an enzyme converting lactate into lactyl-CoA and a gene of a polyhydroxyalkanoate (PHA) synthase using lactyl-CoA as a substrate, and is capable of producing the PLA or hydroxyalkanoate-lactate copolymer using sucrose as a substrate, and wherein the gene of the PHA synthase using lactyl-CoA as the substrate has a base sequence corresponding to an amino-acid sequence in which at least one position selected from the group consisting of E130D, S325T, S477R, S477H, S477F, S477Y, S477G, Q481M, Q481K, and Q481R is mutated in SEQ ID NO: 4; and harvesting the PLA or hydroxyalkanoate-lactate copolymer from the microorganism.