Patent ID: 7179604
Filing Date: 2007-02-20
Classification: C07K,C12N

Abstract:
1. A method for isolating a nucleic acid molecule encoding a homologue of bunion histamine H3 receptor comprising the steps of: (a) mixing a nucleic acid molecule comprising a nucleotide sequence encoding human histamine H3 receptor comprising the amino acid sequence of SEQ ID NO:7 with a sample comprising a nucleic acid molecule encoding a homologue of human histamine H3 receptor; (b) allowing said nucleic acid molecule comprising a nucleotide sequence encoding human histamine H3 receptor to hybridize with said nucleic acid molecule encoding a homologue of human histamine H3 receptor to form a hybridized nucleic acid complex; (c) isolating the hybridized nucleic acid complex; and (d) purifying the nucleic acid molecule encoding a human histamine H3 receptor homologue, wherein said histamine H3 receptor homologue comprises biological activity of human histamine H3 receptor comprising the amino acid sequence of SEQ ID NO:7, wherein said biological activity is selected from binding to a histamine H3 receptor-specific ligand, cyclic AMP induction in the presence of a histamine H3 receptor antagonist, inhibition of adenylate cyclase in response to histamine, or incorporation of GTP-gamma-S.