Patent ID: 8765373
Filing Date: 2014-07-01
Classification: C12Q,G01N

Abstract:
1. A method to determine if a target substance is present in an analyte comprising: providing a plurality of beads attached to a surface of a sensor by a molecular tether configured and arranged to be selectively cleaved in the presence of a target substance to release at least one of the beads from the surface of the sensor wherein the target substance is a single-stranded DNA target and a molecular tether is a hybridized double-stranded DNA tether with one of the single-stranded DNA fragments of the double-stranded DNA tether being complementary to the DNA target; using a magnet positioned above the surface of the sensor to apply a force on the beads in a direction opposite that of a tether and to remove from the surface of the sensor at least one of the beads as a result of the molecular tether being cleaved in a course of a reaction with the target substance while in the field of the force applied by the magnet; and determining by use of a measurement system the population of beads attached to the sensor surface after the introduction of the analyte.