Patent ID: 8647863
Filing Date: 2014-02-11
Classification: C12N

Abstract:
1. A plasmid comprising the following functional units: a prokaryotic origin of replication, a marker sequence, two specific recombinase recognition sequences and a multiple cloning site, a regulatory element for the expression of a recombinase characterised in that it comprises a gene coding for a sequence specific recombinase, a minicircle identification sequence for the identification and isolation of a minicircle and/or a miniplasmid identification sequence for the identification, isolation and removal of a miniplasmid and wherein a gene coding for a specific protein is inserted into the multiple cloning site, whereby the units are arranged on the plasmid in such a way that the plasmid is divided into a miniplasmid and a minicircle upon induction of the expression of the sequence specific recombinase via the regulatory element, said miniplasmid comprising the prokaryotic origin of replication, the marker sequence and the gene for the sequence specific recombinase and said minicircle comprising the multiple cloning site, wherein the sequence specific recombinase is ParA resolvase.