Patent ID: 6083758
Filing Date: 2000-07-04
Classification: G01N

Abstract:
A method of screening peptides to determine if any one peptide coordinates to a metal, comprising the steps of:(a) contacting an array of peptides with a solution containing a known concentration of a metal;wherein said array comprises a plurality of peptides coupled to a solid phase such that peptides of differing amino acid sequence are spatially separated from one another,wherein each of said peptides comprising a sequence of at 6-15 amino acid residues and at least one metal binding group or metal signaling group and wherein one of said peptides may bind a metal and undergo a detectable fluorometric perturbation; and(b) monitoring the fluorescence of each of the peptides in the array;wherein a change in the fluorescence of any one of the peptides is indicative that that peptide coordinates to the metal.