Patent ID: 6448047
Filing Date: 2002-09-10
Classification: C12N

Abstract:
A method for detecting the presence of a nucleotide sequence in nucleic acid of a sample, said method comprising the steps of:(a) providing an aqueous solution comprising one or more lipids for releasing nucleic acid from the sample, said solution further comprising one or more of: i) an enzyme(s) to degrade cell structure; ii) a non-ionic membrane fluidizing compound(s); and iii) a metal chelator(s); wherein said aqueous solution is non-denaturing and non-inhibitory of enzymes or proteins used in and during nucleic acid release, amplification, labeling or detection; (b) contacting the sample with the aqueous solution of step a) under conditions suitable for degrading cell structure and releasing the nucleic acid from the cells; (c) contacting the nucleic acid with one or more nucleic acid probes or primers that are complementary to the nucleic acid to be detected: i) under conditions suitable for the one or more nucleic acid probes to hybridize to the nucleic acid to form a hybridized product; or ii) under conditions suitable for amplification of the nucleic acid to form an amplified product; and (d) detecting the hybridized product by capture or separation from unhybridized nucleic acid probe and nucleic acid of the sample or detecting the amplified nucleotide sequence, whereby the presence of a nucleotide sequence in nucleic acid of a sample is determined.