Patent ID: 6582730
Filing Date: 2003-06-24
Classification: C09B

Abstract:
A natural fluorescent dye obtained from an extract of a marine organism, said organism being a marine sea cucumber Holothuria scabra and said dye having the following characteristics:i. the fluorescent dye is decolorized by a reducing agent, ii. the fluorescent dye decolorized is not a synthetic compound, iii. the crude extract of the dye is yellowish green in color, iv. the purified dye is reddish brown colored powder when seen with the naked eye in the daylight, v. under tube light some hues of green are emitted by said dye, vi. the dye is amorphous in nature, vii. the dye is soluble in water, viii. the dye is insoluble in organic solvents, ix. the dye is negatively charged, x. the dye has a pH of 6.5, xi. the dye comprises a phenolic group, xii. the dye does not have a quinonoid ring, xiii. the dye does not have aromatic amine groups, xiv. the dye is non-proteinaceous in nature, xv. reducing sugar is absent from said dye, xvi. the dye can act as a biosurfactant, xvii. the dye has antimicrobial qualities and when antimicrobial assay is performed, shows zone of inhibition, xviii. pigment cum dye is a fluorescent dye and emits fluorescence when excited with different wavelengths of UV and visible spectral ranges on a spectrophotometer, xix. UV, visible spectroscopy is from 300 nm-700 nm and the peaks are marked at 379 nm and 439 nm wavelengths for said dye, xx. UV, visible spectroscopy from 250 nm-350 nm and the peaks are at 272 nm and 299 nm wavelengths, xxi. fluorescent spectroscopy for said dye in the UV and visible spectra, when excited with UV 270 nm wavelength the fluorescence is emitted in the 324 nm-380 nm range, said range being within the UVA wavelength range of ultraviolet rays of sunlight, xxii. at an excitation wavelength 450 nm in Fluorescent spectroscopy for said dye the fluorescence emission is at 500 nm-580 nm with maximum intensity, xxiii. at an excitation wavelength 540 nm in Fluorescent spectroscopy for said dye the fluorescence emission is at 500 nm-620 nm with maximum intensity, xxiv. with excitation wavelength 555 nm in Fluorescent spectroscopy for said dye the fluorescence emission is at 575 nm-620 nm with maximum intensity, xxv. physical checking of Whatman Filter no. 1 dipped with dye concentration 1:40000 dilution under UV transilluminator and Gel Documentation system with UV bulbs of 260 nm-280 nm range said dye emits a bluish green hue color of fluorescence, xxvi. the dye emits three different colored fluorescence at 3 different wavelengths of the UV and visible ranges of the fluorescent cubes of an epifluorescence microscope, xxvii. fluorescence blue color emission occurs in the 380 nm-400 nm range of UVA when excited under ultraviolet cube WU-330 nm-385 nm excitation range for said dye, xxviii. fluorescence yellow color emission occurs in the 500 nm-570 nm range when excited under WB cube of 450 nm-480 nm excitation range for said dye, xxix. fluorescence orange color emission occurs in the 570 nm-650 nm range when excited under WG cube of 510 nm-550 nm excitation range for said dye, xxx. the dye emits hues of grays under the ordinary transmitted light bulb of the epifluorescence microscope when seen under 10Ã—objective, xxxi. the dye emits fluorescence colors at a dilution range of at least 1:40000, xxxii. the fluorescence of the extract persists after at least 1 year at room temperature, xxxiii. the fluorescence of the dye is photostable and is not deteriorated by long exposures to direct light, and xxxiv. the fluorescence of the dye does not change when frozen at 20Â° C.