Patent ID: 7049100
Filing Date: 2006-05-23
Classification: A01K,C07H,C07K,C12N,C12P,C40B

Abstract:
1. A method for expressing a modular polyketide synthase (PKS) which produces a polyketide or a non-ribosomal peptide synthase (NRPS) that produces a non-ribosomal peptide (NRP) in a host cell comprising: (a) introducing a vector selected from a library of recombinant vectors into a host cell; (i) wherein the host cell further comprises an additional vector or vectors; which in conjunction with the selected vector is capable of producing the polyketide or NRP; or (ii) wherein additional vector or vectors are introduced into the host cell to allow the production of polyketide or NRP; and (b) culturing the host cell into which the vector or vectors have been introduced under conditions such that said PKS or NRPS is expressed; wherein each of said vectors encodes an open reading frame (ORF) of the PKS or NRPS; wherein said library of recombinant vectors comprises (i) a recombinant vector encoding a first functional mutant ORF and (ii) a recombinant vector encoding a second functional mutant ORF of a wild-type ORF; and wherein each of said vectors may be integrative or freely replicating.