Patent ID: 8460866
Filing Date: 2013-06-11
Classification: C12Q

Abstract:
1. A method for the high throughput detection of a plurality of target nucleotide sequences in one or more samples, comprising the steps of: (a) providing for the plurality of target nucleotide sequences, a set of first probes and a set of second probes, (b) for each of the target sequences, allowing the first and second target specific section of the respective first probes and second probes to hybridize to the target sequence, (c) ligating the first and second probes when the respective target specific sections of the probes are hybridized to essentially adjacent sections on the target sequence to provide ligated probes of same or very similar length, (d) optionally, amplifying the ligated probes with a first and a second primer to provide amplicons, (e) subjecting the ligated probes or amplicons to high throughput sequencing to determine at least part of the nucleotide sequence, at least including the identifier sequence(s), of the ligated probes or the amplicons, (f) identifying the presence of the target nucleotide sequence in the sample by determination of the presence or absence of the identifier sequence in the nucleotide sequence of step (e)