Patent ID: 6057120
Filing Date: 2000-05-02
Classification: C07D,C12Q,G01N,Y10S

Abstract:
In a method for the quantitative or qualitative detection of an analyte in a sample comprising contacting the sample with a reagent having a detectable property and quantitatively or qualitatively detecting the analyte by detecting a change in the detectable property of the reagent, the improvement wherein the reagent comprises a compound of formula I and/or formula II ##STR4## wherein R.sup.1 and R.sup.2 are each independently hydrogen, halogen or an organic residue, or together form a substituted or unsubstituted aromatic or heteroaromatic ring system,X is O, S, C(Acc).sub.2, CH(Acc) or N(Acc), wherein Acc is an electron-attracting group,Ph is a phenyl ring which is unsubstituted or substituted,n is 0 to 4, andAr is a group of formula IIIa, IIIb, IVa, IVb, Va, Vb, VIa or VIb: ##STR5## wherein R.sup.3, R.sup.3', R.sup.4, R.sup.4', R.sup.5 and R.sup.5' are each independently hydrogen, halogen or an organic residue, or (a) R.sup.3 and R.sup.4 and/or R.sup.3' and R.sup.4' or (b) R.sup.4 and R.sup.5 and/or R.sup.4' and R.sup.5' in each case together form a ring system comprising an aromatic or heteroaromatic ring,Y and Y' are each independently N or CR.sup.6, wherein R.sup.6 is hydrogen, halogen or an organic residue,Z is NR, O or S, wherein R is hydrogen or an organic residue,R' is in each case independently a substituted or unsubstituted alkyl or aryl residue, andm is in each case independently 0 or 1, wherein when m is 1 then a nitrogen bound to R' in the formulae IIIa, IVa, Va and VIa carries a positive charge and a counterion is present, and p is 0 in the formulae IIIb, IVb, Vb and VIb, and wherein m is 0 then p is 1.