Patent ID: 6063595
Filing Date: 2000-05-16
Classification: C12N,C12Q

Abstract:
A method for forming a double-stranded DNA polymer having repeating subunits in tandem, which comprises:a) forming a first mixture comprising a DNA polymerase and two oligonucleotides, wherein the two oligonucleotides have complementary sequences to each other at the 3'-terminal region of each oligonucleotide, and wherein at least one of the oligonucleotides has one to three mismatched, overhanging nucleotides at its 3' terminus;b) heating and cooling the first mixture under conditions sufficient to allow for annealing of the two oligonucleotides to each other and extension of the oligonucleotides by the DNA polymerase to form a double-stranded DNA polymer, thereby forming a second mixture containing said double-stranded DNA polymer; andc) repeatedly heating and cooling the second mixture to form a double-stranded DNA polymer having repeating units in tandem.