Patent ID: 6423693
Filing Date: 2002-07-23
Classification: A01K,A61K,C07K,C12N

Abstract:
A vector for expression of a nucleic acid sequence in a cell, comprising:a nucleic acid cassette comprising a nucleotide sequence encoding a growth hormone releasing hormone (â€œGHRHâ€ ); a 5â€² flanking region including one or more sequences necessary for expression of said nucleic acid cassette, wherein said sequences include a promoter, a linker nucleic acid sequence linking said 5â€² flanking region to a nucleic acid, said linker nucleic acid sequence comprising a site for inserting said nucleic acid cassette, wherein said linker nucleic acid sequence lacks the coding sequence of a gene with which it is naturally associated; and a 3â€² flanking region operably linked to said linker nucleic acid sequence, wherein the 3â€² flanking region comprises a 3â€² untranslated region (â€œUTRâ€ ) or a 3â€² noncoding region (â€œNCRâ€ ) or both, wherein said 3â€² flanking region is 3â€² to said site for inserting said nucleic acid cassette, wherein said nucleotide sequence encoding said GHRM is a synthetic sequence encoding a human GHRH wherein said human GHRH has the sequence of SEQ ID NO: 2.