Patent ID: 9005964
Filing Date: 2015-04-14
Classification: A01K,A61K,A61P,C12N

Abstract:
1. A method of obtaining endodermal progenitor cells comprising: (a) culturing a cell population obtained from an adult endodermal tissue to induce cluster formation in culture medium; (b) seeding the clusters in a vessel coated with one or more attachment factors, wherein cells comprising the clusters adhere to the one or more attachment factors and form a monolayer; (c) culturing the monolayer clusters in culture medium until a population of endodermal progenitor cells emerges; and (d) isolating the endodermal progenitor cells, wherein the isolated endodermal progenitor cells do not express smooth muscle actin or PDX-1, wherein a function of a cell in the tissue is not reduced by application of an exogenous stressor prior to harvesting the tissue, wherein the exogenous stressor is adverse chemicals, nutritional stressors, cell depletion or physical stress.