Patent ID: 6692911
Filing Date: 2004-02-17
Classification: A61K,C12N

Abstract:
A biocompatible composition comprising:a delivery agent, characterized by an ability to bind to a nucleic acid and mediate import into endosomes, wherein the delivery agent is selected from the group consisting of polylysine, polyhistidine, lysine, histidine, and combinations thereof; the nucleic acid, wherein the delivery agent forms a non-covalent complex with a nucleic acid having a nucleic acid to delivery agent weight:weight ratio in the range of 1:3 to 1:10; and an imidazole-containing endosomal lysing agent, wherein the endosomal lysing agent is free of chloroquine, fusogenic peptides, inactivated adenoviruses, and polyethyleneimine; and wherein the endosomal lysing agent is selected from the group consisting of histidine, histamine, vinylimidazole, polymers thereof, and any combinations of histidine, histamine, vinylimidazole, and polymers thereof.