Patent ID: 8036832
Filing Date: 2011-10-11
Classification: C12Q,G06K,G16B

Abstract:
1. A method for target sequence quantitation, comprising: amplifying a plurality of target sequences by PCR in the presence of a detectable reporter construct; acquiring signal information for each target sequence of the plurality of target sequences amplified by detecting the associated detectable reporter construct; identifying a plurality of exponential regions, wherein each exponential region is associated with substantial increases in the signal information for each target sequence amplification and wherein each identified exponential region comprises an associated upper and lower bound; normalizing the signal information for each target sequence amplification using an identified baseline component for the signal information to compensate for non-specific fluorescence in the signal information; determining an exponential region threshold based on a comparison of the plurality of exponential regions, wherein each exponential region of the plurality of exponential regions is associated with a target sequence amplification of the plurality of target sequence amplifications; performing a polynomial fitting operation to fit a polynomial to at least an interval of the exponential region including the exponential region threshold; identifying, based on the exponential region threshold and an associated polynomial root, a threshold cycle for the at least one target sequence amplification; and quantifying each target sequence based on the threshold cycle.