Patent ID: 6010844
Filing Date: 2000-01-04
Classification: A61P,C12N,C12P,C12Y

Abstract:
A method of preparing and recovering a protein from a pro-protein solution, comprisingproviding a pro-protein-containing solution, wherein the pro-protein is selected from the group consisting of factor II, factor V, factor VII, factor VIII, pro-factor IX, factor IX, factor X, factor XIII, protein C and pro-von Willebrand factor;providing a protease selected from the group consisting of trypsin, chymotrypsin, factor Xa, venom-protease, thrombin, plasmin and a serine-protease of the subtilisin family;providing a solid carrier selected from the group consisting of immobilized hirudin, hirudin derivatives shown in the sequence of SEQ ID NO 2 and SEQ ID NO:3, immobilized heparin, immobilized benzamidine, immobilized antibodies, and immobilized peptides, wherein said solid carrier has a higher affinity to said protein to be recovered than to the pro-protein and to degradation products;contacting said pro-protein-containing solution with said protease and said solid carrier so as to proteolytically cleave said pro-protein to said protein, wherein said contacting of said pro-protein-containing solution with said protease is effected for a period of time sufficient to cleave said pro-protein to said protein, and wherein no further degradation of said protein takes place; andselectively separating said protein by adsorbing said protein on said solid carrier.