Patent ID: 6372225
Filing Date: 2002-04-16
Classification: A61K,A61P,C07K,C12P

Abstract:
A tetanus toxin functional fragment antigen which is stabilized with a fixative, wherein the tetanus toxin functional fragment antigen prior to stabilization with said fixative comprises at least one independent fragment which is obtained by a process comprising the steps ofsplitting at least one peptide bond selected from peptide bonds individually connecting mutually adjacent amino acid residues in a partial amino acid sequence between two cysteine residues participating in forming a disulfide bridge present in the N-terminal of the entire amino acid sequence of a whole tetanus toxin molecule, splitting said disulfide bridge, and splitting non-covalent bonds between groups of the tetanus toxin molecule; said tetanus toxin functional fragment antigen prior to stabilization with said fixative having: (a) a molecular weight of from 90000 to 110000 as measured by an SDS-polyacrylamide gel electrophoresis method; and (b) an isoelectric point of 7.25Â±0.5 as measured by an isoelectric focusing method, wherein each of said at least one independent fragment has an amino acid sequence selected from the group consisting of SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, SEQ ID NO:16, and SEQ ID NO:17.