Patent ID: 8119118
Filing Date: 2012-02-21
Classification: A61K,A61P,C07K,C12N

Abstract:
1. A method of treating a patient who has cancer, or who may develop cancer, the method comprising (a) transducing dendritic cells of the patient ex vivo with an HSV amplicon particle, wherein the particle is made by a helper virus-free method comprising: (i) providing a virus packaging cell that has been stably transfected with a nucleic acid sequence that encodes an accessory protein; and (ii) transfecting the virus packaging cell with one or more packaging vectors that, individually or collectively, encode one or more HSV structural proteins but do not encode a functional herpes virus cleavage/packaging site and an amplicon plasmid comprising a functional herpes virus cleavage/packaging site, a herpes virus origin of DNA replication, and a sequence encoding an immunostimulatory protein under the control of a promoter, thereby producing transduced dendritic cells; and (b) administering the transduced dendritic cells to the patient, wherein the transduced dendritic cells express a tumor specific antigen.