Patent ID: 8722401
Filing Date: 2014-05-13
Classification: C12N

Abstract:
1. A method for the in vitro expansion of a mammal Tr1 cell population P′, from a purified or enriched mammal Tr1 regulatory cell population P, in a culture medium Mp, said expansion requiring the presence of at least five factors in said culture medium Mp, said method comprising: (a) cultivating insect feeder cells at a temperature T (b) contacting the insect feeder cells obtained at step (a) with the Tr1 cell population P in the culture medium Mp, wherein said culture medium Mp does not initially contain the at least five factors, to obtain a mixture containing the Tr1 cell population P, the insect feeder cells and the culture medium Mp, c) cultivating the mixture obtained at step (b), wherein the at least five factors are expressed by the insect feeder cells in the culture medium Mp, and said cultivating is carried out at a temperature T d) recovering the Tr1 cell population P′ so expanded,