Patent ID: 8338155
Filing Date: 2012-12-25
Classification: A61P,C12N,C12P

Abstract:
1. A modified mevalonate kinase comprising an amino acid sequence having at least 95% amino acid sequence identity to the amino acid sequence of SEQ ID NO:1, wherein the amino acid sequence of the modified mevalonate kinase contains one mutation or combination of mutations selected from the group consisting of: (1) combination of P55L and C117S mutations; (2) F59S mutation; (3) combination of N66K and I152M mutations; (4) combination of K83E and S249P mutations; (5) combination of H111N and K375N mutations; (6) combination of L106P and S218P mutations; and (7) combination of I142N, L158S, L231I and T367S mutations wherein the one mutation or combination of mutations of (1) to (7) corresponds to the amino acid sequence of SEQ ID NO: 1 and wherein the modified mevalonate kinase has mevalonate kinase activity and exhibits a sensitivity to feedback inhibition which is reduced by at least 20% in comparison to the corresponding non-modified