Patent ID: 7790444
Filing Date: 2010-09-07
Classification: B01L,C12M

Abstract:
1. A bioreactor for monitoring cellular activity in the presence of physical, chemical and mechanical stimuli, said bioreactor comprising: at least one culture chamber having an inlet and an outlet; a premixing chamber, separated from said at least one culture chamber, to prepare a culture medium; a pH sensor immersed in the culture medium present in said premixing chamber; a circuit connected to said inlet and said outlet and comprising said premixing chamber; means for conveying in a controlled manner said culture medium through said circuit; means for generating at least one physical-chemical stimulus that has to be applied to the cells being tested, said stimulus being selected from the group of temperature, pH, pressure or combination thereof; and means for controlling said means for generating at least one physical-chemical stimulus, so that said or each stimulus reaches predetermined values; wherein a frustum conical concave structure is present in and at the bottom of the premixing chamber, where said pH sensor is arranged, in order to protect said pH sensor from direct contact with bubbles of gas.