Patent ID: 9121064
Filing Date: 2015-09-01
Classification: B82Y,C12Q,G01N

Abstract:
1. A method for sequencing a nucleic acid molecule with a nanopore system comprising: providing a substrate comprising a nanopore in contact with a solution, the solution comprising a template nucleic acid above the nanopore, wherein at least a portion of the sequence of the template nucleic acid is unknown; providing a voltage across the nanopore; measuring the current through the nanopore over time as the template nucleic acid is translated through the nanopore, wherein the translocation rate through the nanopore is enzymatically controlled, wherein the measured current varies depending on whether and which of two or more types of nucleotides are in the nanopore; determining a sequence of the nucleic acid template by comparing the measured current through the nanopore over time with known current over time values for the two or more types of nucleotides in the nanopore.