Patent ID: 7282342
Filing Date: 2007-10-16
Classification: A61K,C07K

Abstract:
1. A method for detecting Gly-Pro-Glu (GPE) in a sample; comprising: (a) providing an antibody raised against said GPE derivatized with a reagent selected from the group consisting of keyhole limpet hemocyanin (KLH), diethyl carbodiimide, 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate, sulfosuccinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate, sulfosuccinimidyl 2-[m-azido-o-nitrobenzamido]-ethyl-1,3′-dithiopropionate and N-succinimidyl-3-[4-hydroxyphenyl]propionate; (b) providing a sample containing an unknown amount of said GPE; (c) derivatizing said GPE in the sample of step (b) using the reagent selected in step (a); (d) permitting said derivatized GPE of step (c) to bind with said antibody of step (a); thereby producing a derivatized GPE-antibody complex; and (e) detecting said derivatized GPE-antibody complex.