Patent ID: 6454710
Filing Date: 2002-09-24
Classification: A61B,B82Y

Abstract:
A device for monitoring an analyte, comprising:a support having an interior surface and an exterior surface; a substrate connected to the interior surface of the support, wherein the substrate is opaque; a spacer connected to me interior surface of the support; a labeled analogue, which binds reversibly to the substrate; and a first membrane having an interior surface and an exterior surface, the interior surface being connected to the spacer; wherein the first membrane is permeable to the analyte and impermeable to the labeled analogue, and the first membrane is substantially transparent to each of an excitation wavelength and an emission wavelength of a fluorescent label; a dye absorbs a majority of the excitation and emission wavelengths of the fluorescent label; the interior surface of the support, the spacer, and the interior surface of the first membrane define a chamber which encloses the substrate and the labeled analogue, the chamber further comprises a reference; and the spacer exceeds the substrate in elevation such that a void volume exists between the interior surface of the first membrane and the substrate.