Patent ID: 8986925
Filing Date: 2015-03-24
Classification: C12Q,G01N

Abstract:
1. An in vitro method for detection and/or quantification and/or identification of viruses present in a fluid medium M constituting a biological material, in which, in a known manner, a suspension of microbeads in a liquid suspension medium is prepared, said microbeads being delimited by an outer surface constituted by a solid polymer material capable of binding proteins, the method comprising the following stages: a) a loading of the microbeads in the suspension with β2GPI proteins is ensured by coupling with a sufficient quantity of β2GPI proteins, either passively in a suspension medium or using a known chemical binding protocol; b) in a container, said microbeads loaded with β2GPI proteins are brought into contact with the fluid medium M by adding ions of at least one oxidizing metal, under appropriate conditions in order to ensure sufficient binding of the viruses to the β2GPI proteins carried by the microbeads; c) the microbeads thus prepared are separated from their suspension medium, said suspension medium is removed from the container in order to obtain, optionally after washing with a buffer, a residue with a high concentration of viruses; d) and the viruses are detected and/or quantified and/or identified, starting from the concentrated residue thus obtained.