Patent ID: 8012691
Filing Date: 2011-09-06
Classification: C12Q

Abstract:
1. A method for simultaneously quantitating human nuclear DNA and human male DNA in a sample and ascertaining the accuracy of said quantitation, the method comprising: combining the sample under amplification conditions with primer pairs capable of specifically amplifying a 60-500 base pair sequence comprising human specific RPPH1, and a 60-500 base pair sequence comprising human male specific SRY loci; an internal plasmid control (IPC) comprising SEQ ID NO:1, and a primer pair capable of amplifying a 60-500 base pair sequence comprising SEQ ID NO:1; labeled probes for detecting the amplified RPPH1, SRY, and SEQ ID NO:1; amplifying the loci and plasmid sequence by a multiplex real-time polymerase chain reaction (PCR); and determining the amount of the amplified loci and plasmid sequence, wherein the amount of amplified loci provides a quantitative value of the amount of the amplified loci and the amount of the amplified plasmid sequence indicates the accuracy of said quantitation.