Patent ID: 8946511
Filing Date: 2015-02-03
Classification: C12N,Y02A

Abstract:
1. A recombinant DNA construct comprising a heterologous promoter functional in a plant cell and operably linked to DNA encoding an RNA transcript consisting essentially of at least one miRNA decoy sequence that is recognized and bound by an endogenous mature plant miRNA but not cleaved, wherein said miRNA decoy sequence is an RNA sequence of between about 19 to about 36 contiguous RNA nucleotides, wherein said miRNA decoy sequence is recognized and bound by said endogenous mature plant miRNA, resulting in base-pairing between said miRNA decoy sequence and said endogenous mature plant miRNA, thereby forming a cleavage-resistant RNA duplex comprising at least one mismatch between said miRNA decoy sequence and said endogenous mature plant miRNA at positions 9, 10, or 11 of said endogenous mature plant miRNA, or at least one insertion at a position in said miRNA decoy sequence corresponding to positions 10-11 of said endogenous mature plant miRNA.