Patent ID: 8945584
Filing Date: 2015-02-03
Classification: A61K,A61P,C12N

Abstract:
1. A recombinant nucleic acid molecule which encodes human hepatitis C virus of genotype 3a/JFH1, wherein said molecule: (i) is capable of expressing said virus when transfected into cells, (ii) is capable of infectivity in vivo, and (iii) encodes an amino acid sequence with at least 99% sequence identity to that of SEQ ID NO: 2, and which (iv) comprises sequences encoding structural genes (Core, E1, E2), p7 and nonstructural gene (NS2) from a genotype 3a HCV strain, and sequences encoding the 5′ untranslated region (UTR), nonstructural genes NS3, NS4A, NS4B, NS5A, NS5B, and the 3′ UTR from the JFH-1 HCV strain and either (a) at least one adaptive mutation in the amino acid sequences of p7 or NS3, selected from the group consisting of I793S, and K1404Q, or, (b) at least two adaptive mutations in the amino acid sequences of E2, p7, NS3 or NS5A selected from the group consisting of M405V, K523Q, I793S, I1793T, Y794C, K1404Q, Q1502L, and S2274P, wherein a p7 adaptive mutation is combined with another adaptive mutation in NS3 or NS5A, and wherein said mutation is numbered according to the HCV sequence of SEQ ID NO: 2.