Patent ID: 6124138
Filing Date: 2000-09-26
Classification: B01L,C12Q,G01N,Y10S

Abstract:
A method for detecting or quantitating one or more of a plurality of polynucleotide sequences in a liquid sample, said method comprisingproviding a device comprising a substrate defining a sample-distribution network having (i) a sample inlet, (ii) two or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of said chambers and said inlet, wherein at least two of said detection chambers each contain a different, sequence-specific polynucleotide binding polymer for detecting or quantitating different polynucleotide sequences that may be present in such sample, to produce a detectable signal, wherein the interior of the network is under vacuum,applying a liquid sample to the sample inlet and allowing the sample to be drawn into the network by vacuum action to deliver the sample to the detection chambers,allowing the delivered sample to react with at least one sequence-specific polynucleotide binding polymer in each detection chamber under conditions effective to produce a detectable signal in each detection chamber when a specific sequence is present in the sample, andmeasuring the signals produced in the reaction chambers to detect or quantitate specific target sequences in the sample.