Patent ID: 6462254
Filing Date: 2002-10-08
Classification: C07K,C12N,C12Y

Abstract:
A method of detecting the expression of a fusion protein in a mammal, the method comprising:(a) introducing into the mammal a construct comprising a nucleic acid encoding the fusion protein, wherein the fusion protein encoded by the construct is expressed, said fusion protein comprising a polypeptide sequence of interest, a capture tag sequence, and a detection tag sequence, wherein the capture tag sequence and the detection tag sequence are each heterologous to the polyp eptide sequence of interest; (b) capturing the fusion protein from a sample from the mammal with a compound that specifically binds the capture tag sequence; thereby prosuing a captured sample, and (c) detecting the fusion protein from in the captured sample of step b with a second compound that specifically binds the detection tag sequence, wherein detection of the fusion protein in the sample indicates the presence of the fusion protein in the mammal.