Patent ID: 6291194
Filing Date: 2001-09-18
Classification: G01N

Abstract:
A method for determining the androgenic activity of a sample, comprising the steps ofa) contacting the sample with a cell comprisinga luciferase reporter plasmid,a fusion protein comprising a ligand-binding domain of the androgen receptor anda Gal4 DNA-binding domain, said fusion protein being able to bind to binding sites in said luciferase reporter plasmid,a fusion protein comprising an N-terminal region of the androgen receptor and a transcriptional activation domain, andan androgen receptor-interacting protein 3,b) allowing the sample to incubate with said cell,c) lysing said cell,d) measuring the luciferase activity of the lysate,e) comparing the measured luciferase activity to that obtained by repeating the steps a) to d) above except that a control is added instead of the sample, to give the relative luciferase activity of the sample, andf) using the relative luciferase activity to detect or quantify an active androgen in the sample.