Patent ID: 6803369
Filing Date: 2004-10-12
Classification: A61K,C07D

Abstract:
A method of modulating the activity of a mammalian type II topoisomerase enzyme comprising contacting said enzyme with a compound of formula (Ia) or a pharmaceutically acceptable derivative thereof: wherein:one of Z1, Z2, Z3, Z4 and Z5 is N, one is CR1a and the remainder are CH, or one of Z1, Z2, Z3, Z4 and Z5 is CR1a and the remainder are CH; R1 is selected from hydroxy; (C1-6) alkoxy optionally substituted by (C1-6)alkoxy, amino, piperidyl, guanidino or amnidino optionally N-substituted by one or two (C1-6)alkyl, acyl or (C1-6)alkylsulphonyl groups, NH2CO, hydroxy, thiol, (C1-6)alkylthio, heterocyclylthio, heterocyclyloxy, arylthio, aryloxy, acylthio, acyloxy or (C1-6)alkylsulphonyloxy; (C1-6)alkoxy-substituted (C1-6)alkyl; halogen; (C1-6)alkyl; (C1-6)atkylthio; nitro; azido; acyl; acyloxy; acylthio; (C1-6)alkylsulphonyl; (C1-6)alkylsulphoxide; arylsulphonyl; arylsulphoxide or an amino, piperidyl, guanidino or amidino group optionally N-substituted by one or two (C1-6)alkyl, acyl or (C1-6)alkylsulphonyl groups, or when one of Z1, Z2, Z3, Z4 and Z5 is N, R1 may instead be hydrogen; R1a is selected from H and the groups listed above for R1; R3 is hydrogen; or R3 is in the 2- or 3-position and is: carboxy; (C1-6)alkoxycarbonyl; aminocarbonyl wherein the amino group is optionally substituted by hydroxy, (C1-6)alkyl, hydroxy(C1-6)alkyl, aminocarbonyl(C1-6)alkyl, (C2-6)alkenyl, (C1-6)alkylsulphonyl, trifluoromethylsulphonyl, (C1-6)alkenylsulphonyl, (C1-6)alkoxycarbonyl, (C1-6)alkylcarbonyl, (C2-6)alkenyloxycarbonyl or (C2-6)alkenylcarbonyl and optionally further substituted by (C1-6)alkyl, hydroxy(C1-6)alkyl, aminocarbonyl(C1-6)alkyl or (C2-6)alkenyl; cyano; tetrazolyl; 2-oxo-oxazolidinyl optionally substituted by R10; 3-hydroxy-3-cyclobutene-1,2-dione-4-yl; 2,4-thiazolidinedione-5-yl; tetrazol-5-ylaminocarbonyl; 1,2,4-triazol-5-yl optionally substituted by R10; or 5-oxo1,2,4-oxadiazol-3-yl; or R3 is in the 2- or 3-position and is (C1-4)alkyl or ethenyl substituted with any of the groups listed above for R3 and/or 0 to 3 groups R12 independently selected from: thiol; halogen; (C1-6)alkylthio; trifluoromethyl; azido; (C1-6)alkoxycarbonyl; ( C1-6)alkylcarbonyl; (C2-6)alkenyloxycarbonyl; (C2-6)alkenylcarbony); hydroxy optionally substituted by (C1-6)alkyl, (C2-6)alkenyl, (C1-6)alkoxycarbonyl, (C1-6)alkylcarbonyl, (C2-6)alkenyloxycarbonyl,(C2-6)alkenylcarbonyl or amninocarbonyl wherein the amino group is optionally substituted by (C1-6)alkyl, (C2-6)alkenyl, (C1-6)alkylcarbonyl or (C2-6)alkenylcarbonyl; amino optionally mono- or disubstituted by (C1-6)alkoxycarbonyl, (C1-6)alkylcarbonyl, (C2-6)alkenyloxycarbonyl, (C2-6)alkenylcarbonyl, (C1-6)alkyl, (C2-6)alkenyl, (C1-6)alkylsulphonyl, (C2-6)alkenylsulphonyl or aminocarbonyl wherein the amino group is optionally substituted by (C1-6)alkyl or (C2-6)alkenyl; aminocarbonyl wherein the amino group is optionally substituted by (C1-6)alkyl, hydroxy(C1-6)alkyl, aminocarbonyl(C1-6)alkyl, (C2-6)alkenyl, (C1-6)alkoxycarbonyl, (C-6)alkylcarbonyl, (C2-6)alkenyloxycarbonyl or (C2-6)alkenylcarbonyl and optionally further substituted by (C1-6)alkyl, hydroxy(C1-6)alkyl, aminocarbonyl(C1-6)alkyl or (C2-6)alkenyl; oxo; (C1-6)alkylsulphonyl; (C2-6)alkenylsulphonyl;(C1-6)aminosulphonyl wherein the amino group is optionally substituted by (C1-6)alkyl or (C2-6)alkenyl; provided that when R3 is disubstituted with hydroxy or amino and carboxy containing substituents these may optionally together form a cyclic ester or amide linkage, respectively; wherein R10 is selected from (C1-4)alkyl; (C2-4)alkenyl; aryl; a group R12 as defined above; carboxy; aminocarbonyl wherein the amino group is optionally substituted by hydroxy, (C1-6)alkyl, (C2-6)alkenyl, (C1-6)alkylsulphonyl, trifluoromethylsulphonyl, (C1-6)alkenylsulphonyl, (C1-6)alkoxycarbonyl, (C1-6)alkylcarbonyl, (C2-6)alkenyloxycarbonyl or (C2-6)alkenylcarbonyl and optionally further substituted by (C1-6)alkyl or (C2-6)alkenyl; cyano; or tetrazolyl; R4 is a group â€”CH2-R5 in which R5 is selected from: (C3-12)alkyl; hydroxy(C3-12)alkyl; (C1-12)alkoxy(C3-12)alkyl; (C1-12)alkanoyloxy(C3-12)alkyl; (C3-6)cycloalkyl(C3-12)alkyl; hydroxy-, (C1-12)alkoxy- or (C1-12)alkanoyloxy-(C3-6)cycloalkyl(C3-12)alkyl; cyano(C3-12)alkyl; (C2-12)alkenyl; (C2-12)alkynyl; tet or di-(C1-12)alkylarnino(C3-12)alkyl; acylamino(C3-12)alkyl; (C1-12)alkyl- or acyl-aminocarbonyl(C3-12)alkyl; mono- or di-(C1-12)alkylamino(hydroxy) (C3-12)alkyl; optionally substituted phenyl(C1-2)alkyl, phenoxy(C1-2)alkyl or phenyl(hydroxy)(C1-2)alkyl; optionally substituted diphenyl(C1-2)alkyl; optionally substituted phenyl(C2-3)alkenyl; optionally substituted benzoyl or benzoyl(C1-3)alkyl; optionally substituted heteroaryl or heteroaryl(C1-2)alkyl;and optionally substituted heteroaroyl or heteroaroylmethyl; n is 0, 1 or2; AB is NR11CO,COâ€”CR8R9 or CR6R7â€”CR8R9 or when n is 1 or 2, AB may instead be Oâ€”CR8R9 or NR11â€”CR8R9, or when n is 2 AB may instead be CR6R7â€”NR11 or CR6R7â€”O, provided that when n is 0, B is not CH(OH), and wherein: each of R6 and R7R8 and R9 is independently selected from: H; thiol; (C1-6)alkylthio; halo; trifluoromethyl; azido; (C1-6)alkyl; (C2-6)alkenyl; (C1-6)alkoxycarbonyl; (C1-6)alkylcarbonyl; (C2-6)alkenyloxycarbonyl; (C2-6)alkenylcarbonyl; hydroxy, amino or arninocarbonyl optionally substituted as for corresponding substituents in R3; (C1-6)alkylsulphonyl; (C2-6)alkenylsulphonyl; or (C1-6)aminosulphonyl wherein the amino group is optionally substituted by (C1-6)alkyl or (C1-6)alkenyl; or R6 and R8 together represent a bond and R7 and R9 are as above defined; and each R11 is independently H, trifluoromethyl, (C1-6)alkyl, (C1-6)alkenyl, (C1-6)alkoxycarbonyl, (C1-6)alkylcarbonyl, aminocarbonyl wherein the amino group is optionally substituted by (C1-6)alkoxycarbonyl, (C1-6)alkylcarbonyl, (C1-6)alkenyloxycarbonyl, (C2-6)alkenylcarbonyl, (C1-6)alkyl or (C1-6)alkenyl and optionally further substituted by (C1-6)alkyl or (C1-6)alkenyl; or where one of R3 and R6, R7, R8 or R9 contains a carboxy group and the other contains a hydroxy or amino group they may together form a cyclic ester or amide linkage, wherein the said compound inhibits enzyme-mediated cleavage of a polynucleotide substrate.