Patent ID: 8852869
Filing Date: 2014-10-07
Classification: G01N

Abstract:
1. A method of in vitro determining functional deficiencies in the lectin pathway of the complement system, the method comprises the steps of (a) diluting a mammalian sample of body fluid with a diluent comprising polyanethol sulphonate (PAS) in a concentration range of from about 50 to about 1000 μg/ml; (b) activating the lectin pathway of the complement system in the sample obtained from (a); and (c) determining in the sample obtained from (b), the activation of one or more of the complement factors C3, C4, or one or more of the components of the C5-C9 complex comprising contacting the sample obtained from (b) with an antibody to one or more of the activated complement factors C3 or C4, or one or more of the components of the C5-C9 complex; and (d) comparing the determined activation level of one or more of the complement factors C3, C4, or one or more of the components of the C5-C9 complex with a reference level, wherein a lower activation level in the sample relative to the reference level is indicative of a functional deficiency in the lectin pathway.