Patent ID: 8603949
Filing Date: 2013-12-10
Classification: C12N

Abstract:
1. A nucleic acid library of cytochrome P450 genes that encode proteins having a first set of intramolecular amino acid contacts, wherein said proteins are derived from: a first parental cytochrome P450 gene that encodes a parental protein having a second set of intramolecular amino acid contacts; and a second parental cytochrome P450 gene, wherein less than 30 amino acid contacts differ between said first set and said second set of intramolecular amino acid contacts thereby providing genes that encode proteins with an E value of less than 30, and wherein said proteins encoded by the library of cytochrome P450 genes have enzymatic activity against a substrate, and wherein the P450 genes in the library are chimeric combinations of the first parental cytochrome P450 gene and the second parental cytochrome P450 gene, and wherein the encoded proteins have an average m value that is at least 22.