Patent ID: 8673314
Filing Date: 2014-03-18
Classification: A61K,A61P,C07K,C12N,G01N

Abstract:
1. A method for generating a monoclonal antibody that binds to and neutralizes two or more strains of an Influenza A virus of the H1 or H3 subtype, comprising (i) administering two, three, four or more immunogenic compositions to a non-human subject, with the administration of each immunogenic composition separated by a certain amount of time, and (ii) generating B-cell hybridomas from the subject and selecting for hybridoma clones that produce a monoclonal antibody that binds to and neutralizes two or more strains of an Influenza A virus of the H1 or H3 subtype, wherein each immunogenic composition comprises an inactivated Influenza virus having a hemagglutinin (HA) polypeptide of the H1 or H3 subtype, an attenuated Influenza virus having an HA polypeptide of the H1 or H3 subtype, a live Influenza virus having an HA polypeptide of the H1 or H3 subtype other than an attenuated Influenza virus having an HA polypeptide of the H1 or H3 subtype, an HA polypeptide or fragment thereof derived or obtained from an Influenza A virus of the H1 or H3 subtype, or a nucleic acid encoding an HA polypeptide or fragment thereof derived or obtained from an Influenza virus of the H1 or H3 subtype, and wherein one immunogenic composition differs from another immunogenic composition in that the HA polypeptide of the H1 or H3 subtype of the Influenza virus, the HA polypeptide of the H1 or H3 subtype or fragment thereof, or the HA polypeptide of the H1 or H3 subtype or fragment thereof encoded by the nucleic acid are antigenically distinct.