Patent ID: 8980621
Filing Date: 2015-03-17
Classification: B01L

Abstract:
1. A high-density multiwell-plate for performing thermocycled amplification reactions of polynucleotides in liquid samples, said plate comprising: a plurality of reaction wells for thermal processing and nucleic acid amplification of the liquid samples and said plate being designed to be thermally processed by a thermal cycling means of an apparatus for analyzing the liquid samples; a substantially planar bottom layer for providing a thermal contact of the plate to a thermal cycling means; a rigid well-forming structure placed above the bottom layer defining single wells, wherein the well-forming structure comprises rigid substantially horizontal well-covering areas that cover the liquid sample comprised in the wells at the top side of the liquid sample; a substantially planar cover placed above or on the top side of the well-forming structure, wherein the cover provides a sealing cover of the wells and a thermal insulating air distance between the well-covering areas of the well-forming structure and the cover; and at least a first filling and venting structure and a second filling and venting structure configured to be coupled to the substantially planar bottom layer and the substantially planar cover and configured so that the first filling and venting structure and the second filling and venting structure are located on opposite sides of the rigid well-forming structure, forming a filling channel comprising a filling opening and a venting channel comprising a venting opening and configured such that a liquid sample placed in the filling opening flows into the fluid channel and under the rigid well-forming structure to fill the well.