Patent ID: 7894998
Filing Date: 2011-02-22
Classification: G16B

Abstract:
1. A method of identifying candidate probe sequences for a nucleic acid sequence for use as probes in a nucleic acid array, said method comprising: (a) providing a set of known, undesirable probe sequences present in a format representative of the nucleotide sequences of said known, undesirable probe sequences, wherein said set of undesirable probe sequences comprises probe sequences that do not meet a predetermined specificity of binding affinity for said nucleic acid sequence; (b) comparing said nucleic acid sequence present in a format representative of the nucleotide sequence of said nucleic acid sequence to each member of said set; (c) marking regions of said nucleic acid sequence that have no more than 5 mismatches with a sequence of a member of said set as undesirable regions for candidate probe sequences for said nucleic acid sequence; (d) identifying candidate probe sequences for said nucleic acid sequence for use as probes in a nucleic acid array from unmarked regions of said nucleic acid sequence; and (e) synthesizing at least one of said identified candidate probe sequences.