Patent ID: 8080410
Filing Date: 2011-12-20
Classification: B01L,G01N

Abstract:
1. A method of measuring the binding of a first partner in an interaction to a second partner in said interaction, wherein said interaction partners are molecular entities, said method comprising the steps of a) delivering a quantity of said second interaction partner to a reaction well of a device for attachment of said second interaction partner to an attachment zone of said reaction well, wherein said device comprises b) adding a solution comprising a quantity of said first interaction partner to said attachment zone and incubating at a temperature and for a time sufficient to allow binding of said first interaction partner to said second interaction partner; c) rotating said device rotor at a speed sufficient to displace the solution comprising unbound first interaction partner from said attachment zone; and d) measuring the amount of said first interaction partner bound to said second interaction partner via the fluorescence or absorbance of said first interaction partner or an indicator molecule bound thereto.