Patent ID: 8697382
Filing Date: 2014-04-15
Classification: C12Q,G01N

Abstract:
1. A method of identifying a metallo-β-lactamase-producing bacteria, comprising: spotting a metallo-β-lactamase inhibitor represented by the following Formula (1) onto a surface of a solid medium coated with a bacteria to be tested, spotting 3 or more β-lactam agents differing in reactivity to metallo-β-lactamase, at positions different from the spot of the metallo-β-lactamase inhibitor, culturing the solid medium, and identifying the metallo-β-lactamase-producing bacteria based on a degree of inhibition affected by a presence of the metallo-β-lactamase inhibitor on each of the 3 or more β-lactam agents, wherein each of the 3 or more β-lactam agents has different reactivity to metallo-β-lactamases, and wherein the degree of inhibition is based on a shape of an inhibition zone determined by at least one value as an index selected from the minimum radius of the inhibition zone, a maximum radius of a inhibition zone, and a ratio between the minimum and maximum radiuses of the inhibition zone.