Patent ID: 8846334
Filing Date: 2014-09-30
Classification: C12M,C12Q

Abstract:
1. A method of detecting acid-producing bacteria, comprising: providing a thin film culture device comprising a cold water-soluble gelling agent, a culture medium to support the growth of lactic acid bacteria, a pH indicator with a transition range that extends below pH 7.0, a carbohydrate that can be fermented by acid-producing bacteria, and a sample suspected of containing acid producing bacteria; combining a predetermined volume of sample and the culture medium to form a first mixture; combining, in the culture device, the first mixture, the pH indicator, and the fermentable carbohydrate; incubating the culture device for a period of time; and detecting the presence or absence of a microorganism; wherein incubating the culture device comprises incubating the device aerobically; wherein detecting the presence of a microorganism comprises differentiating a microorganism; wherein differentiating a microorganism comprises detecting a gas bubble associated with the microorganism.