Patent ID: 6596515
Filing Date: 2003-07-22
Classification: A61K,A61P,C07K,C12N

Abstract:
A method for preparing an insulin expression vector, in which an insulin gene amplified from human genomic DNA by use of a pair of insulin primers represented by the following sequences (I) and a K14 promoter gene amplified by use of a pair of K14 primers represented by the following sequences (II) are inserted together into pUChsneo:Insulin forward(I)5â€²â€ƒCCTGCCTGTCTCCCAGAGCTCTGTCCTTCT3â€², SEQ ID NO. 3Insulin reverse5â€²â€ƒGCAGGGCTGGTTCTAGAGCTTTATTCCATC3â€², SEQ ID NO. 4 K14 promoter Forward(II)5â€²â€ƒATTGCTGAAGTTTTGATCTAGACACCTCCA3â€², SEQ ID NO. 5K14 promoter Reverse5â€²â€ƒCTGAGTGAAGAGAAGGAGCTCGGGTAAATT3â€², SEQ ID NO. 6.