Patent ID: 6114147
Filing Date: 2000-09-05
Classification: C07K,C12N

Abstract:
A method for immobilizing a binding protein or a functional part thereof, which binding protein or functional part thereof specifically binds to a compound, wherein said binding protein or functional part thereof is immobilized by being anchored to the cell wall of a fungus selected from the group consisting of yeasts and molds, said method comprisingrecombinantly modifying said fungus to produce a chimeric protein, which chimeric protein comprisessaid binding protein or functional part thereof,a secretory signal peptide operably linked to said binding protein or functional part thereof anda C-terminal anchoring part of a fungal cell wall anchoring protein operably linked to said binding protein or functional part thereof,wherein, when said chimeric protein is produced, said chimeric protein is transported to said cell wall by said signal peptide and said C-terminal anchoring part is anchored to said cell wall, thereby immobilizing said binding protein or a functional part thereof.