Patent ID: 7534569
Filing Date: 2009-05-19
Classification: C12N

Abstract:
1. A method of generating a single-stranded nucleic acid molecule, the method comprising: (a) combining in a mixture under conditions suitable for a polymerase extension reaction: (b) hybridizing the initial polynucleotide or the extension polynucleotide and one of the template oligonucleotides; (c) incubating the mixture for sufficient time to allow an extension polynucleotide to be synthesized; (d) adding a competimer that competes with the template oligonucleotide in step (b); (e) denaturing the extension polynucleotide and template oligonucleotide; and (f) repeating steps (b), (c), (d), and (e) to generate the single-stranded nucleic acid molecule, wherein the number of repeated cycles equals the number of different template oligonucleotides.