Patent ID: 7592420
Filing Date: 2009-09-22
Classification: C07K,C12N

Abstract:
1. A composition comprising a protease inhibitor selected from the group consisting of: a Bowman-Birk inhibitor (BBI) comprising SEQ ID NO:7 wherein residue 13 is Ala or is changed to Ser, Gly or Gln, and wherein residues 15-21 and/or 42-48 have been replaced with a variant sequence; and a Soybean Trypsin Inhibitor (STI) comprising SEQ ID NO:12 wherein residues 40-85 and/or 137-144 have been replaced with a variant sequence; wherein said variant sequence is selected from the group consisting of: a) introducing a DNA construct into a filamentous fungal cell, wherein said DNA construct comprises a promoter showing transcription activity in the filamentous fungal cell and which is operably linked to a heterologous DNA sequence encoding the protease inhibitor, b) culturing the filamentous fungal cell under suitable culture conditions to allow expression of the heterologous DNA sequence, and c) producing the protease inhibitor.