Patent ID: 6300084
Filing Date: 2001-10-09
Classification: A01N,C07K

Abstract:
A method for screening potential anti-mitotic agents comprising:a) providing one or more purified proteins that are required for yeast mitotic spindle formation, wherein the one or more proteins are selected from the group consisting of a protein encoded by the yeast gene YGR113w (Dam I), and proteins provided in a complex of the Duo 1 protein encoded by the yeast gene YGL061 c and the Dam I protein encoded by the yeast gene YGR113w,b) combining in vitro said one or more proteins with microtubules, and a potential anti-mitotic agent;c) incubating said one or more proteins, said microtubules, and said anti-mitotic agent;d) assaying the microtubules for binding of said one or more proteins; ande) identifying an anti-mitotic agent, wherein said anti-mitotic agent inhibits binding of said one or more proteins to said microtubules.