Patent ID: 8822147
Filing Date: 2014-09-02
Classification: C12Q,G01N,Y10T

Abstract:
1. A method of detecting multiple targets in a biological sample, comprising: (a) contacting the biological sample with a first plurality of probes capable of binding a first group of different targets in the biological sample; (b) binding the first plurality of probes to their respective targets; (c) detecting signals from the first plurality of probes; (d) applying an oxidizing agent after step (c) to modify the signals; (e) contacting the biological sample after step (d) with a second plurality of probes capable of binding a second group of different targets in the biological sample; (f) binding the second plurality of probes to their respective targets; and (g) detecting signals from the second plurality of probes; and wherein each probe comprises an antibody and a fluorophore.