Patent ID: 6485903
Filing Date: 2002-11-26
Classification: C07H,C12Q,Y10S

Abstract:
An oligonucleotide probe comprising:an oligonucleotide capable of hybridizing to a target polynucleotide sequence; a fluorescer molecule attached to a first location on the oligonucleotide; a quencher molecule attached to a second location on the oligonucleotide such that the quencher molecule substantially quenches the fluorescer molecule whenever the oligonucleotide probe is not hybridized to the target polynucleotide sequence and such that the fluorescer molecule is substantially unquenched whenever the oligonucleotide probe is hybridized to the target polynucleotide sequence; a 5â€² end which is rendered impervious to digestion by a 5â€²â†’3â€² exonuclease activity of a polymerase; and a 3â€² end which is rendered impervious to a 5â€²â†’3â€² extension activity of the polymerase.