Patent ID: 7846455
Filing Date: 2010-12-07
Classification: A61K,C07K,C12N

Abstract:
1. An infectious chimeric respiratory syncytial virus (RSV) comprising a major nucleocapsid (N) protein, a nucleocapsid phosphoprotein (P), a large polymerase protein (L), a RSV M2 ORF1 RNA polymerase elongation factor (M2(ORF1)), and a partial or complete genome or antigenome of RSV A comprising genome segments encoding antigenic determinants of RSV B F and G glycoproteins, wherein the RSV A genome or antigenome contains the following attenuating mutations: i) a mutation encoding V267I in the N gene; ii) a mutation encoding C319Y in the L gene; iii) a mutation encoding H1690Y in the L gene; iv) a mutation encoding Q831L in the L gene; v) a mutation encoding D1183E in the L gene; vi) a point mutation T to C at nucleotide 7605 of the gene start of the M2 gene; and wherein the RSV A genome or antigenome further contains at least one of the following: