Patent ID: 6962796
Filing Date: 2005-11-08
Classification: A61K,C07K,C12N

Abstract:
1. A process for the production of purified recombinant intact hPTH, comprising the steps of: (a) providing a microorganism that is engineered genetically to produce exogenous and intact hPTH(1-84), wherein said microorganism is selected from the group consisting of (b) expressing said intact hPTH(1-84) within said microorganism, wherein said microorganism comprises DNA encoding hPTH(1-84); and (c) purifying said intact hPTH(1-84) so as to produce an intact hPTH(1-84), wherein the intact hPTH(1-84) so produced: (1) reacts with antibodies against human PTH in a manner identical to the native hPTH(1-84) hormone, (2) has the molecular weight of the native hPTH(1-84) hormone, and (3) migrates as a band of approximately 9000 daltons when subjected to gel electrophoresis; wherein purified recombinant intact hPTH(1-84) is produced.