Patent ID: 9081006
Filing Date: 2015-07-14
Classification: B01D,G01N

Abstract:
1. A method for detecting the presence of a target molecule in a sample performed entirely in solution phase, comprising: a) detecting the presence of a target molecule in a sample by subjecting the sample, to determine if the sample contains the target molecule, to electrophoresis entirely in a conductive aqueous solution phase, further comprising wherein the target molecule, when present in the sample, binds to the first and second binding agents to form a target complex, wherein the electrophoresis is performed wherein the sample layer comprises the target molecule, detection conjugate and capture conjugate; wherein the electrophoresis moves (i) non-complexed and (ii) complexed molecules comprising said target complex through the capture layer, wherein the capture layer comprises uncharged liquid polymers that bind a cognate binding agent provided in the capture molecule; wherein the electrophoresis moves said target complex through the capture layer and separates non-complexed molecules from the complexed molecules comprising the target complex that are bound to the uncharged liquid polymers through the cognate binding agent of the capture molecule; wherein the electrophoresis moves said complex through the stacking layer, wherein the stacking layer comprises uncharged liquid polymers that impede the mobility of complexes bound to the uncharged liquid polymers that becomes concentrated and immobile in the stacking layer in order to separate the non-complexed molecules from the complexed molecules comprising the target complex that are bound to the uncharged liquid polymers; and wherein the presence of the target complex is detected by measuring or detecting the amount or presence of the detection molecule provided in the target complex.