Patent ID: 9080201
Filing Date: 2015-07-14
Classification: C12Q,G01N

Abstract:
1. A method for determining cholesterol in high-density lipoprotein 3 (HDL3) contained in a sample, which comprises reacting, in an aqueous medium containing (a) a divalent metal salt, (b) an alkali metal sulfate, and (c) dextran sulfate or a salt thereof, the sample with (1) a combination of a cholesterol ester hydrolase and a cholesterol oxidase or (2) a combination of a cholesterol ester hydrolase, an oxidized coenzyme and a cholesterol dehydrogenase; wherein the concentration of alkali metal ions derived from the alkali metal sulfate in the reaction solution is 5 to 21 mmol/L; measuring a substance formed or consumed in the reaction; correlating a calibration curve indicating a relationship between a concentration of cholesterol in HDL3 and an information amount derived from the substance formed or consumed with the measurement value of the substance formed or consumed; and determining the concentration of cholesterol in HDL3 contained in the sample, wherein the method is carried out without separating and removing lipoproteins other than HDL3 from the sample.