Patent ID: 6146836
Filing Date: 2000-11-14
Classification: C07K,G01N

Abstract:
A method for determining the amount of analyte in a patient sample while reducing interference by endogenous immunoglobulins or complement proteins present in the sample, said method comprising:a. combining the patient sample with (i) a binding protein specific for said analyte, said binding protein having a specific allotype, and (ii) detectably labeled tracer molecules specific for said binding protein;b. incubating the combination formed in step (a) under conditions sufficient to permit the analyte and the labeled tracer molecules to bind to said binding protein;c. contacting the combination with a solid phase having immobilized thereon a capture antibody specific for the binding protein allotype wherein the capture antibody is obtained from hybridoma cell line ATCC 12349 or ATCC 12350;d. incubating the combination formed in step (c) under conditions sufficient to permit the binding protein to bind with the capture antibody;e. separating the solid phase from the sample; andf. detecting the amount of labeled tracer molecules bound to the solid phase;wherein said amount of labeled tracer detected is indicative of the amount of analyte present in the sample, and wherein the use of the allotype-specific antibody in step (c) reduces interference by endogenous immunoglobulins or complement proteins present in the sample.