Patent ID: 8658211
Filing Date: 2014-02-25
Classification: A01K,A61K,C12N

Abstract:
1. An RNA interference polynucleotide delivery polyconjugate for delivering the RNA interference polynucleotide to a mammalian cell in vivo comprising: a membrane inactive reversibly modified amphipathic membrane active random copolymer wherein A is a hydrophobic group-containing monomer, B is a primary amine-containing monomer, C comprises an RNA interference polynucleotide linked to a primary amine-containing monomer via a physiologically labile linkage orthogonal to maleamate, D comprises a targeting ligand linked to a primary amine-containing monomer via a maleamate linkage having the structure —NH—CO—C(R)═C(L)—COO E comprises a steric stabilizer linked to a primary amine-containing monomer via a maleamate linkage having the structure —NH—CO—C(R′)═C(SS)—COO a is an integer greater than zero, b is an integer greater than or equal to two, c is an integer greater than or equal to one, d is an integer greater than or equal to zero, e is an integer greater than or equal to zero, the value of d+e is greater than 50% of the value of b, and poly(A