Patent ID: 8535930
Filing Date: 2013-09-17
Classification: C07K,C12N

Abstract:
1. A method of producing HPV L1 polypeptides in a plant comprising the steps of: cloning a HPV L1 gene or nucleic acid encoding a functional equivalent thereof into a vector adapted to target a polypeptide expressed in cytoplasm to chloroplasts present in the plant; infiltrating at least a portion of the plant with the vector or transforming plant tissue with the vector so as to transiently express the HPV L1 polypeptides in the cytoplasm and then import the HPV L1 polypeptides into the chloroplasts, and/or to create a transgenic plant wherein the HPV L1 polypeptides are expressed in the cytoplasm and then imported into the chloroplasts; and recovering the HPV L1 polypeptides expressed by the plant.