Patent ID: 7445924
Filing Date: 2008-11-04
Classification: A61K,C07K,C12N

Abstract:
1. A poxvirus preparation obtained by a process comprising: cultivating primary avian cells permissive for productive replication of the virus in a serum free medium comprising a factor selected from the group consisting of growth factors and attachment factors; infecting the primary avian cells with the virus; cultivating the infected cells in serum free medium until progeny virus is produced; and isolating the virus from the culture wherein the poxvirus is an MVA-derived vaccinia virus characterized by replicating in vitro in chicken embryo fibroblasts and by being non-replicative in vitro in human cells which permit replication of MVA vaccinia strain 575 (ECACC V00120707) and MVA vaccinia strain 572 (ECACO V94012707).