Patent ID: 6638717
Filing Date: 2003-10-28
Classification: C12N,C12Q

Abstract:
A method of enhancing the rate of novel gene discovery in a population of previously-uncharacterized nucleic acid molecules, comprisinga. contacting an first ordered array of nucleic acid samples from said population with an initial complex subtraction probe pool, said probe pool comprising a labeled probe corresponding to a redundant or known sequence; b. identifying a nucleic acid, said nucleic acid being characterized by weak or no hybridization to said initial complex subtraction probe pool; c. sequencing said nucleic acid to yield a newly-identified sequence; d. adding said newly-identified sequence to said initial pool to generate a second complex subtraction probe pool, said second pool comprising a larger number of labeled probes compared to said initial pool; e. contacting a second ordered a array of nucleic acid samples from said population with said second subtraction probe pool; and f. repeating steps b.-e., wherein each repeat of steps b.-e. reduces the redundancy of said population of previously-uncharacterized nucleic acid molecules, thereby enhancing the rate of novel gene discovery.