Patent ID: 7008664
Filing Date: 2006-03-07
Classification: A23D,A23K,C11B,C11C,C12N

Abstract:
1. A method of feeding an animal comprising feeding the animal a corn grain obtained from a transgenic corn plant comprising in its genome a chimeric gene selected from the group consisting of: (i) a first chimeric gene comprising an isolated nucleic acid fragment encoding a corn delta-9 stearoyl ACP desaturase comprising the amino acid sequence set forth SEQ ID NO:9 wherein all or a part of the first isolated nucleic acid is useful in co-suppressing an endogenous gene encoding a corn delta-9 stearoyl ACP desaturase, or the complement thereof, and linked to a second chimeric gene comprising a second isolated nucleic acid fragment encoding a corn delta-12 desaturase wherein said second fragment has a nucleic acid sequence identity of at least 90% based on the Clustal method of alignment when compared to a nucleic acid as set forth in SEQ ID NO:1, wherein all or a part of the isolated second nucleic acid fragment is useful in co-suppressing an endogenous gene encoding a corn delta-12 desaturase, or the complement thereof, operably linked to at least one regulatory sequence; (ii) a chimeric gene comprising (a) a first isolated nucleic acid fragment encoding a corn delta-9 stearoyl ACP desaturase comprising the amino acid sequence set forth in SEQ ID NO:9 wherein all or a part of the isolated first nucleic acid fragment is useful in co-suppressing an endogenous gene encoding a corn delta-9 stearoyl ACP desaturase, or the complement thereof, (b) a second isolated nucleic acid fragment encoding a corn delta-12 desaturase wherein said second fragment has a nucleic acid sequence identity of at least 90% based on the Clustal method of alignment when compared to a nucleic acid as set forth in SEQ ID NO:1, wherein all or a part of the isolated second nucleic acid fragment is useful in co-suppressing an endogenous gene encoding a corn delta-12 desaturase, or the complement thereof, and (c) a third isolated nucleic acid fragment comprising a corn oleosin promoter comprising the nucleotide sequence set forth in any of SEQ ID NOS: 38–40 and 42–49 wherein the isolated nucleic acid fragments of (a), (b) and (c) are operably linked; (iii) a chimeric gene comprising (a) a first isolated nucleic acid fragment encoding a corn delta-9 stearoyl ACP desaturase comprising the amino acid sequence set forth in SEQ ID NO:9 wherein all or a part of the isolated first nucleic acid fragment is useful in co-suppressing an endogenous gene encoding a corn delta-9 stearoyl ACP desaturase, or the complement thereof, (b) a second isolated nucleic acid fragment encoding a corn delta-12 desaturase wherein said second fragment has a nucleic acid sequence identity of at least 90% based on the Clustal method of alignment when compared to a nucleic acid as set forth in SEQ ID NO:1, wherein all or a part of the isolated second nucleic acid fragment is useful in co-suppressing an endogenous gene encoding a corn delta-12 desaturase, or the complement thereof, and (c) a shrunken 1 intron/exon, operably linked to at least one regulatory sequence wherein (a), (b) and (c) are operably linked; or (iv) a chimeric gene comprising (a) a first isolated nucleic acid fragment encoding a corn delta-9 stearoyl ACP desaturase comprising the amino acid sequence set forth in SEQ ID NO:9, or the complement thereof, (b) a second isolated nucleic acid fragment encoding a corn delta-12 desaturase wherein said second fragment has a nucleic acid sequence identity of at least 90% based on the Clustal method of alignment when compared to a nucleic acid as set forth in SEQ ID NO:1, or the complement thereof, (c) a third isolated nucleic acid fragment comprising a corn oleosin promoter comprising the nucleotide sequence set forth in any of SEQ ID NOS: 38–40 and 42–49, and (d) a shrunken 1 intron/exon wherein (a), (b), (c) and (d) are operably linked; and further wherein expression of the chimeric gene results in an altered corn oil phenotype compared to the oil phenotype of a non transgenic corn grain.