Patent ID: 8216779
Filing Date: 2012-07-10
Classification: G01N

Abstract:
1. A method to test a composition comprising cells that do not contain PrP-res for the presence or absence of cells containing an infective TSE agent which method comprises (a) adding said composition of cells to be tested to a culture of a neuronal cell line receptive to TSE infection, wherein the neuronal cell line has been modified to be resistant to an antibiotic, (b) co-culturing the cells in said neuronal composition with said cell line, (c) treating the co-culture to which the said neuronal cell line is resistant with said antibiotic, and (d) determining the presence, absence, or level of PrP-res in said co-cultured neuronal cell line, whereby the presence of PrP-res in the neuronal cell line indicates the presence of a TSE infective agent-containing cells in the composition tested.