Patent ID: 6713257
Filing Date: 2004-03-30
Classification: B01J,C12Q,C40B,G16B

Abstract:
A method of identifying the location of exons within the genome of a species of organism comprising:(a) contacting a sample comprising RNAs or nucleic acids derived therefrom from one or more cells of said species of organism with an array, said array comprising a positionally-addressable ordered array of polynucleotide probes bound to a solid support, said polynucleotide probes comprising a first plurality of at least 100 polynucleotide probes of different, predetermined nucleotide sequences, each said different nucleotide sequence comprising a sequence complementary and hybridizable to a different genomic sequence of the same species of organism, said respective genomic sequences for the probes being found at sequential predetermined sites in said genome of said species of organism, said contacting being under conditions conducive to hybridization between said RNAs or nucleic acids derived therefrom and said probes; (b) identifying the one or more probes to which hybridization of one or more of said RNAs or nucleic acids derived therefrom occurs; and (c) identifying said genomic sequences for each said identified probe as the location of an exon within the genome of said species of organism.