Patent ID: 6150101
Filing Date: 2000-11-21
Classification: A61K,A61P,C07K,C12N

Abstract:
A method for identifying a composition which affects TGF-.beta. induced connective tissue growth factor (CTGF) expression, the method comprising:(a) incubating components comprising the composition, TGF-.beta., and a nucleic acid molecule comprising an expressible nucleotide sequence operably linked to a TGF-.beta. regulatory element (T.beta.RE),said T.beta.RE comprising the nucleotide sequence 5'-GTGTCAAGGGGTC-3' (SEQ ID NO: 8),wherein the incubating is carried out under conditions and for a time sufficient to allow the components to interact; and(b) comparing expression of the expressible nucleotide sequence operably linked to the T.beta.RE in the presence of the composition with the level of expression of the nucleotide sequence in the absence of the composition, wherein a difference in the levels of expression identifies a composition that affects TGF-.beta. induced CTGF expression.