Patent ID: 6500615
Filing Date: 2002-12-31
Classification: C12N,C12Q

Abstract:
A method for identifying an antisense oligonucleotide capable of binding to a target mRNA, comprising:(a) contacting the target mRNA with each member of an oligonucleotide library separately under hybridization conditions, wherein each member of the oligonucleotide library consists essentially of a set of oligonucleotides, each oligonucleotide comprising a central known sequence flanked on both sides by one or more flanking bases, wherein the central known sequence consists of a first fixed number of four or five bases of known sequence, the flanking bases consist of a second fixed number of between 2 and 8 bases, and the oligonucleotides are a common length of no more than 12 bases, and wherein the oligonucleotides of a set comprise all possible combinations of the flanking bases, and wherein each member of the oligonucleotide library comprises a different central known sequence and all possible combinations of central known sequence are present in the oligonucleotide library; (b) optionally removing unhybridized material; (c) determining which member or members hybridize; and (d) comparing the known sequence of each member which hybridizes with the known sequence of the target mRNA so as to identify in the target MRNA one or more antisense binding sites.