Patent ID: 8962298
Filing Date: 2015-02-24
Classification: C12N,C12P,Y02E

Abstract:
1. A recombinant microbial host cell comprising heterologous DNA molecules encoding polypeptides that catalyze the substrate to product conversions below: i) pyruvate to alpha acetolactate; ii) alpha acetolactate to acetoin; iii) acetoin to 2,3-butanediol; and iv) 2,3-butanediol to 2-butanone; wherein said microbial host cell produces 2-butanol; and wherein the polypeptide that catalyzes the substrate to product conversion of 2,3-butanediol to 2-butanone is diol dehydratase or glycerol dehydratase, comprising an amino acid sequence having at least 95% identity to an amino acid sequence comprising all three of the amino acid sequences encoding large, medium and small subunits, as set forth in SEQ ID NO:8, SEQ ID NO:10, and SEQ ID NO:12; based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250 series of protein weight matrix.