Patent ID: 7482118
Filing Date: 2009-01-27
Classification: C12Q

Abstract:
1. A method for detecting the presence of a target nucleic acid molecule by detecting non-target cleavage products, comprising: a) providing: b) combining said Pfu FEN-1 nuclease, said target nucleic acid, said first oligonucleotide, and said aromatic moiety under reaction conditions to form a cleavage complex, wherein said first portion of said first oligonucleotide is annealed to said first region of said target nucleic acid to form a duplex, wherein said Pfu FEN-1 nuclease recognizes said duplex, and wherein said aromatic moiety interacts with said Y33 residue of said Pfu FEN-1 nuclease, such that cleavage of said complex occurs to generate a non-target cleavage product; and c) detecting the cleavage of said cleavage complex.