Patent ID: 9200319
Filing Date: 2015-12-01
Classification: A61P,C07H,C12Q

Abstract:
1. A method of identifying a plurality of bases in a target nucleic acid, comprising the following steps: (i) attaching the 5′-end of a primer to a solid surface; (ii) hybridizing a target nucleic acid to the primer attached to the solid surface; (iii) adding a compound of the formula: (iv) adding a nucleic acid replicating enzyme to the hybridized primer/target nucleic acid complex to incorporate the compound of step (iii) into the growing primer strand, wherein the incorporated compound of step (iii) terminates the polymerase reaction at an efficiency of between about 70% to about 100%; (v) washing the solid surface to remove unincorporated components; (vi) detecting the reporter group of the incorporated compound of step (iii); (vii) a photochemical cleavage step to remove the terminating moiety of the incorporated compound, wherein the terminating moiety has the formula: (viii) washing the solid surface to remove the cleaved terminating moiety; and (ix) repeating steps (iii) through (viii) one or more times to identify the plurality of bases in the target nucleic acid.