Patent ID: 8198407
Filing Date: 2012-06-12
Classification: C07K

Abstract:
1. A method of sequential protein isolation and purification comprising: (i) providing a plasma sample, (ii) providing a plurality of ligands each of which binds specifically to a target protein in the plasma sample, wherein each of the plurality of ligands is optionally attached to a support to form a plurality of ligand-support complexes, (iii) contacting the plurality of ligands or ligand-support complexes in a predetermined order with the plasma sample to allow each ligand or ligand-support complex to bind its respective target protein from the plasma sample, wherein the predetermined order of contacting the plurality of ligands or ligand-support complexes with the plasma sample results in the binding of plasminogen before fibrinogen and fibrinogen before IgG and IgG before albumin, and wherein the plasma sample is not processed through any pre-conditioning step that includes alcohol precipitation, cryoprecipitation, removal of lipids and/or lipid proteins, euglobulin precipitation, or a combination thereof prior to said contacting the plurality of ligands or ligand-support complexes, (iv) eluting the respective target proteins bound to each of the plurality of ligands or ligand-support complexes, and (v) isolating the respective target proteins.