Patent ID: 6620587
Filing Date: 2003-09-16
Classification: C07K,C12N

Abstract:
A method capable of (I) displaying and selecting proteins and peptides and (II) recovering genetic material selected from the group consisting of DNA and mRNA that encodes the proteins or peptides, wherein the method comprises:(a) transcribing DNA and translating mRNA therefrom in a eukaryotic cell free system in order to form complexed particles, wherein a complexed particle comprises at least one individual nascent protein or peptide associated with (i) one or more ribosomes and (ii) the mRNA encoding the protein or peptide; (b) contacting the complexed particles with a ligand, antigen or antibody; (c) selecting complexed particles using binding of the ligand, antigen or antibody to the protein or peptide of the complexed particle; and (d) conducting reverse transcription and polymerase chain reaction (RT-PCR) on the mRNA bound in the selected complexed particle from step (c) in order produce DNA encoding the protein or peptide.