Patent ID: 9150897
Filing Date: 2015-10-06
Classification: C07K,C12N,C12P

Abstract:
1. A method for recombinantly producing a protein, comprising the steps of: (1) introducing an expression cassette into a host cell, wherein the expression cassette comprises a polynucleotide sequence encoding a protein of interest and at least two additional coding sequences, wherein the at least two additional coding sequences encode maltose binding protein (MBP) tags, or wherein the at least two additional coding sequences encode glutathione-S-transferase (GST) tags, such that the expression cassette encodes a fusion protein comprising the protein of interest and at least two MBP or GST tags located at the N-terminus and/or C-terminus of the protein of interest; and (2) maintain the cell under conditions permissible for the expression of the fusion protein, whereby producing the fusion protein.