Patent ID: 6602687
Filing Date: 2003-08-05
Classification: A61K,A61P,C07K

Abstract:
A method for producing biologically active human ciliary neurotrophic factor in bacteria, comprising the steps of(a) culturing a bacterial cell transformed with a bacterial expression vector comprising the DNA ATGGCTTTCACAGAGCATTCACCGCTGACCCCTCACCGTCGGGACCTCTGTAGCC GCTCTATCTGGCTAGCAAGGAAGATTCGTTCAGACCTGACTGCTCTTACGGAATC CTATGTGAAGCATCAGGGCCTGAACAAGAACATCAACCTGGACTCTGCGGATGG GATGCCAGTGGCAAGCACTGATCAGTGGAGTGAGCTGACCGAGGCAGAGCGACT CCAAGAGAACCTTCAAGCTTATCGTACCTTCCATGTTTTGTTGGCCAGGCTCTTA GAAGACCAGCAGGTGCATTTTACCCCAACCGAAGGTGACTTCCATCAAGCTATA CATACCCTTCTTCTCCAAGTCGCTGCCTTTGCATACCAGATAGAGGAGTTAATGA TACTCCTGGAATACAAGATCCCCCGCAATGAGGCTGATGGGATGCCTATTAATGT TGGAGATGGTGGTCTCTTTGAGAAGAAGCTGTGGGGCCTAAAGGTGCTGCAGGA GCTTTCACAGTGGACAGTAAGGTCCATCCATGACCTTCGTTTCATTTCTTCTCATC AGACTGGGATCCCAGCACGTGGGAGCCATTATATTGCTAACAACAAGAAAATG, â€ƒwherein said bacterial expression vector is capable of being replicated in said bacterial cell and capable of directing expression of said DNA in said bacterial cell to produce human ciliary neurotrophic factor; (b) causing human ciliary neurotrophic factor to be produced in said bacterial cell; and (c) recovering biologically active human ciliary neurotrophic factor from said bacterial cell.