Patent ID: 9017657
Filing Date: 2015-04-28
Classification: A61K

Abstract:
1. A method to induce transformation of human islet cell cluster from human mesenchymal stem cells, comprising, (i) isolating human umbilical cord mesenchymal stem cells (HUMSCs) from human umbilical cord; (ii) culturing the isolated HUMSCs in a medium containing serum; (iii) culturing the cultured HUMSCs in mammalian neuronal cell conditioned medium (NCM) containing cytosine arabinoside (AraC) to produce nestin-positive cells; (iv) culturing the nestin-positive cells in DMEM/F12 medium containing 2% serum, nicotinamide (NAD) and vitamin B27 to produce islet-like cell clusters (ICCs); and (v) culturing the ICCs in DMEM/F12 medium containing 2% serum, NAD and vitamin B27 supplement with mesenchymal stem cell conditioned medium (SCM) containing one or more active components selected from the group consisting of interleukin-6 (IL-6), tissue inhibitor of metalloproteinase-1 (TIMP-1), tissue inhibitor of metalloproteinase-2 (TIMP-2), monocyte chemoattractant protein-1 (MCP-1), growth related oncogene (GRO), hepatocyte growth factor (HGF), insulin-like growth factor binding protein 4 (IGFBP-4) and interleukin-8 (IL-8) to produce ICCs expressing insulin and glucagon.