Patent ID: 8399203
Filing Date: 2013-03-19
Classification: G01N

Abstract:
1. A method of analyzing an analyte, the method comprising: providing a cellular sample comprising a chemically crosslinked analyte, wherein the sample comprises one or more chemically fixed tissue sections embedded in paraffin, wherein the chemically crosslinked analyte comprises a protein or peptide; affixing at least a portion of the sample to a substrate; separating the sample from the paraffin; reversing at least a portion of the chemical crosslinks in the protein or peptide of the crosslinked analyte and forming a decrosslinked analyte, wherein substantially no naturally occurring bonds in the sample are reversed during said reversing at least a portion of the chemical crosslinks, and wherein said reversing at least a portion of the chemical crosslinks occurs after the sample has been affixed to the substrate; and analyzing the analyte by analyzing the protein or peptide of the decrosslinked analyte using mass spectrometry.