Patent ID: 8455260
Filing Date: 2013-06-04
Classification: C12Q,G16B,Y10T

Abstract:
1. A method for determining a sequence of a biomolecule, the method comprising the steps of: a) creating a biomolecule fragment from the biomolecule; b) binding a plurality of uniform probes having the same sequence and length to the biomolecule fragment; c) identifying a plurality of binding sites along the biomolecule fragment at which the probes bind to the biomolecule; d) creating a collection of binding signatures for the biomolecule fragment, each binding signature representing a series of distances between binding sites within the fragment; e) grouping the binding signatures into a plurality of signature clusters based at least in part on distances between the binding sites in each binding signature; f) for each binding signature in a first cluster, selecting a potential successor binding signature from signature clusters other than the first signature cluster; g) identifying one of the potential successor binding signatures as a successor binding signature; h) repeating steps f) and g) until the successor signature represents a terminal signature, resulting in a sequence of signatures representing at least a portion of the biomolecule.