Patent ID: 8105771
Filing Date: 2012-01-31
Classification: B01J,B01L,B82Y,C12Q,G01N

Abstract:
1. A method for determining all or part of the sequence of a plurality of target nucleic acid sequences comprising: (a) providing an array comprising a plurality of different target nucleic acid sequences randomly distributed on a surface of said array, wherein each target nucleic acid comprises a first target domain and an adjacent second target domain, wherein said first and second target domains each comprise multiple nucleotides; (b) contacting said array with: wherein at least one of said first and second probe pools comprises a label; (c) ligating probes from said first probe pool and probes from said second probe pool when hybridized to said first and second target domains to form first ligated probes; (d) detecting said first ligated probes to determine at least one nucleotide of said first target domain; (e) removing said first ligated probes; (f) repeating steps (b)-(d) to determine all nucleotides of said first target domain, wherein each nucleotide of said first target domain is determined multiple times.