Patent ID: 6596510
Filing Date: 2003-07-22
Classification: C07K,C12N

Abstract:
A process for producing S-layer proteins, which comprises(a) preparing a Gram-negative prokaryotic host cell which is transformed with a nucleic acid which codes for an S-layer protein from a non-Gram negative bacteria and is operatively linked to a signal sequence which codes for a peptide which brings about integration of the S-layer protein in the outer membrane of the host cell, integration of the S-layer protein in the cytoplasmic membrane of the host cell, secretion of the S-layer protein into the periplasmic space of the host cell or/and secretion into the medium surrounding the host cell, (b) cultivating the host cell under conditions leading to expression of the nucleic acid and to production of the polypeptide encoded thereby, and (c) where appropriate isolating the resulting polypeptide from the outer membrane of the host cell, from the cytoplasmic membrane of the host cell from the periplasmic space of the host cell or/and from the medium surrounding the host cell.