Patent ID: 8614072
Filing Date: 2013-12-24
Classification: C12P,C12Q

Abstract:
1. A process for amplifying an oligonucleotide sequence, said process comprising contacting said oligonucleotide sequence in aqueous solution with a forward primer comprising a first part at its 5′-end and a second part at its 3′-end, a reverse primer comprising a first part at its 5′-end and a second part at its 3′-end, a forward external primer identical in sequence to a segment within the first part of the forward primer, and a reverse external primer identical in sequence to a segment within the first part of the reverse primer, wherein the second part of said forward primer is complementary to a region at the 3′-end of said oligonucleotide sequence, wherein the second part of the reverse primer is identical in sequence to a region at the 5′-end of said oligonucleotide sequence, wherein the forward external primer and reverse external primer have independently selected sequences that contain at least one non-standard nucleotide incorporating a nucleobase selected from the group consisting of wherein R is the point of attachment of said nucleobase to the sugar of said non-standard nucleotide, and incubating the mixture with an enzyme selected from the group consisting of a DNA polymerase, an RNA polymerase, and a reverse transcriptase, and triphosphates that said enzyme incorporates opposite each of the nucleotides in said primers and said amplified oligonucleotide sequence.