Patent ID: 7145058
Filing Date: 2006-12-05
Classification: A01N,C12N

Abstract:
1. A method of inhibiting growth of genetic transformant bacteria Agrobacterium tumefaciens after transformation in plants selected from the group consisting of tea, carnations, and roses, said method comprising: (1) inoculating (2) incubating the culture in darkness for a time duration of 10–20 hours at a temperature of 20–35° C. and at a revolution rate of 100–300 rpm; (3) after said incubation, harvesting the culture at 0.6–0.8 optical density at 600 nm for concentration of 1×10 (4) pelleting live bacterial cells in the harvested culture by centrifuging the harvested culture for a time duration of 15–40 minutes at a revolution rate of 3000–9000 rpm and at a temperature of 20–35° C., thereby forming a pellet; (5) suspending the pellet in a fresh yeast mannitol broth medium to form a bacterial suspension; (6) optimizing bacterial cell density at 1×10 (7) immersing one or more explants of one or more plants in the bacterial suspension for a time duration of from 3 to 40 minutes, the explants being selected from the group consisting of leaf, somatic embryo, callus and zygotic axes; (8) removing excess genetic transformant (9) incubating the explants obtained in step (8) in an incubation medium for a time duration of from 1 to 20 days; (10) preparing a crude extract of tea by a process comprising: (11) transferring the one or more explants of step (9) to a regeneration medium containing from 1 to 1000 micrograms/ml of the crude tea extract prepared in step (10); and (12) maintaining the one or more explants in said regeneration medium in darkness at 25–30° C. at 150–200 rpm for 48 hours, thereby obtaining one or more explants upon which growth of genetic transformant bacteria