Patent ID: 8192995
Filing Date: 2012-06-05
Classification: G01N,Y10T

Abstract:
1. A method of correction of particle interference to hemoglobin measurement of a blood sample comprising: (a) mixing an aliquot of a blood sample with a lytic reagent to lyse red blood cells, and forming a sample mixture; (b) measuring absorbance of said sample mixture at a predetermined wavelength of a hemoglobin chromogen formed in said sample mixture, and obtaining an apparent hemoglobin concentration of said blood sample using obtained absorbance; (c) measuring a first concentration and a first size of a first subpopulation of cellular particles of a first type remaining in said sample mixture; (d) measuring a second concentration and a second size of a second subpopulation of cellular particles of a second type remaining in said sample mixture; (e) determining a corrected hemoglobin concentration of said blood sample by removing a contribution of said first and second subpopulations of cellular particles to said apparent hemoglobin concentration, the determining based on at least said first measured concentration, said first measured size, a first size factor, said second measured concentration, said second measured size, and a second size factor, said first and second size factors being different from each other, the first size factor being based, at least in part, on the shape of particles of the first type, the second size factor being based, at least in part, on the shape of particles of the second type; and (f) reporting said corrected hemoglobin concentration of said blood sample.