Patent ID: 6677433
Filing Date: 2004-01-13
Classification: A21D,A23C,A23J,A23K,A23L,A61K,A61P,C07K,C12N,C12P,C12Q,C12Y

Abstract:
A method of producing an allergenic protein with reduced allergenicity comprising:(a) treating an allergenic protein containing disulfide bonds with an amount of thioredoxin, nicotinamide adenine dinucleotide phosphate-thioredoxin reductase and NADPH, or an amount of dithiothreitol effective to and at a temperature effective to reduce at least one disulfide bond in said allergenic protein; and (b) reacting the allergenic protein from step (a) with an amount of a physiological disulfide effective to minimize reoxidation of at least one reduced disulfide bonds in said allergenic protein from step (a).