Patent ID: 7906106
Filing Date: 2011-03-15
Classification: A61K

Abstract:
1. A method of monitoring inflammatory cell migration in a mammal comprising the steps of: (a) providing a donor mammalian subject; (b) introducing an inflammatory agent into the donor mammal to create a granuloma; (c) isolating granuloma-derived nucleated cells from the granuloma of the donor mammalian subject; (d) labeling the granuloma-derived nucleated cells of step (c) with a fluorescent dye, wherein the fluorescent dye is selected from the group consisting of Cy5, Cy5.5, Cy7, Cy7.5, CypHer, IRDye 800, IRDye 680, Atto 647, Atto 655, Atto 740, Dy652, Dy734, Alexa 647, Alexa 680, or Alexa 750; (e) providing a recipient mammalian subject with inflamed tissue; (f) introducing the granuloma-derived nucleated cells of step (d) into the recipient mammalian subject at a site remote from the inflamed tissue; and (g) imaging the recipient mammalian subject using an optical system capable of detecting the fluorescent dye.