Patent ID: 8303945
Filing Date: 2012-11-06
Classification: A61P,C07K

Abstract:
1. An isolated interleukin-1 receptor antagonist (IL-Ra) polypeptide wherein the polypeptide comprises the amino acid sequence selected from the group consisting of: (a) SEQ ID NO: 1 with mutations P38L, D47N, E52R, E75K, R92G, K96M and V143I; (b) SEQ ID NO: 1 with mutations P38L, D47N, E52R, E75K and K93E; (c) SEQ ID NO: 1 with mutations D47N, E52R, L78F and K93E; (d) SEQ ID NO: 1 with mutations E52R and K93Q; (e) SEQ ID NO: 1 with mutations P38Y, D47N, E52R, E90Y and M136N; (f) SEQ ID NO: 1 with mutations P38Y, D47N, E52R, H54R, E90Y and M136N; (g) SEQ ID NO: 1 with mutations D47N, E52R, H54R and E90Y; (h) SEQ ID NO: 1 with mutations D47N, E52R, H54R, E90Y, Q129L and M136N; and (i) SEQ ID NO: 1 with mutations P38L, E52K, E75K, K93Q and V143I j) SEQ ID NO: 1 with mutations P38L, D47N, E52K and K93E; (k) SEQ ID NO: 1 with mutations D47N, E52K, E75K and K93Q; (l) SEQ ID NO: 1 with mutations P38L and E52K; (m) SEQ ID NO: 1 with mutations E52R; (n) SEQ ID NO: 1 with mutations E52K; (o) SEQ ID NO: 1 with mutations E52K and T109I; (p) SEQ ID NO: 1 with mutations E52K and P107T; (q) SEQ ID NO: 1 with mutations P38L, D47N, E52R, L78F and K93E; (r) SEQ ID NO: 1 with mutations P38L, D47N, E52K, R92G and K93E; (s) SEQ ID NO: 1 with mutations D47N, E52K, H54R, E75K, K93Q; (t) SEQ ID NO: 1 with mutations D47S, E52K, P53S, E75K and K93Q; (u) SEQ ID NO: 1 with mutations P38L, E52K, E75K and K93Q; (v) SEQ ID NO: 1 with mutations P38L, E52K, E75K, K93Q and Q129L; (w) SEQ ID NO: 1 with mutations P38L, E52K, E75K, Q79L and K93Q; (x) SEQ ID NO: 1 with mutations P38L, E52K, E75K, E90G, K93E and D104Y; (y) SEQ ID NO: 1 with mutations P38L, E52K, K93E and K96M; (z) SEQ ID NO: 1 with mutations P38L, E52K, A82T, K93E and S110R; (aa) SEQ ID NO: 1 with mutations P38L, D47N, E52K, E75K, K93Q and D138G; (bb) SEQ ID NO: 1 with mutations P38L, K45Q, D47N, E52K, E75K, K93Q and V143I; (cc) SEQ ID NO: 1 with mutations E52K and K93E; (dd) SEQ ID NO: 1 with mutations P38L, E52K, E75K and K93Q; (ee) SEQ ID NO: 1 with mutations P38Y, E52R and E90Y; (ff) SEQ ID NO: 1 with mutations P38Y, E52R, E90Y and M136N; (gg) SEQ ID NO: 1 with mutations P38Y, E52R, H54R, E90Y and M136N; (hh) SEQ ID NO: 1 with mutations P38Y, D47N, E52R, E90Y, Q129L and M136N; (ii) SEQ ID NO: 1 with mutations E52R, E90Y and M136N; (u) SEQ ID NO: 1 with mutations P38L, L42F, E52K, E75K, K93Q and M136K (kk) SEQ ID NO: 1 with mutations D47N, E52K, E75K, K93Q and K145E; (11) SEQ ID NO: 1 with mutations P38L, E52K and N84S; (mm) SEQ ID NO: 1 with mutations P38L, E52K and K93E; (nn) SEQ ID NO: 1 with mutations P38L, D47N, E52R, L78F, K93E and V143I; (oo) SEQ ID NO: 1 with mutations P38L, D47N, E52R, R92G, K96M and V143I; (pp) SEQ ID NO: 1 with mutations P38L, D47N, E52R, R92G and K96M; (qq) SEQ ID NO: 1 with mutations P38L, L78F and K93E; (n) SEQ ID NO: 1 with mutations P38L, D47N, E52R, E75K, L78F and K93E; (ss) SEQ ID NO: 1 with mutations P38L, D47N, E52R, E75K and K93Q; (tt) SEQ ID NO: 1 with mutations P38L, E52K, R92G and K96M; (uu) SEQ ID NO: 1 with mutations P38L, E52K, E75K and K93Q; (vv) SEQ ID NO: 1 with mutations P38L, D47S, E52K, P53S, E75K, R92G and K96M; (ww) SEQ ID NO: 1 with mutations P38L, R92G and K96M; (xx) SEQ ID NO: 1 with mutations D47N, E52R, R92G and K96M; (yy) SEQ ID NO: 1 with mutations E52R, L78F, N84D and K93E; (zz) SEQ ID NO: 1 with mutations E52R, E75K, R92G, K96M and V143I; (aaa) SEQ ID NO: 1 with mutations L78F, A82V, R92G and K96M; (bbb) SEQ ID NO: 1 with mutations D47S, E52K, P53S, E75K, R92G and K96M; (ccc) SEQ ID NO: 1 with mutations D47S, E52K, P53S, R92G and K96M; (ddd) SEQ ID NO: 1 with mutations E75K, R92G, K96M and V143I; (eee) SEQ ID NO: 1 with mutations E75K and K93Q; (fff) SEQ ID NO: 1 with mutations E75K, E90G, K93Q and V143I; (ggg) SEQ ID NO: 1 with mutations E75K and K93E; (hhh) SEQ ID NO: 1 with mutations R92G, K96M and V143I; (iii) SEQ ID NO: 1 with mutations R92G and K96M; (jjj) SEQ ID NO: 1 with mutations P38Y and E52R; (kkk) SEQ ID NO: 1 with mutations P38Y, E52R and M136N; (lll) SEQ ID NO: 1 with mutations P38Y, E90Y and M136N; (mmm) SEQ ID NO: 1 with mutations P38Y, D47N, E52R and Q129L; (nnn) SEQ ID NO: 1 with mutations P38Y, H54R, E90Y and Q129L; (ooo) SEQ ID NO: 1 with mutations P38Y, P53S, Q129L and M136N; (ppp) SEQ ID NO: 1 with mutations P38Y, D47N, E52R and M136N; (qqq) SEQ ID NO: 1 with mutations P38Y, E52R, H54R and E90Y; (rrr) SEQ ID NO: 1 with mutations P38Y, E52R, P53S, Q129L and M136N; (sss) SEQ ID NO: 1 with mutations P38Y, P53S, H54R, E90Y and Q129L; (ttt) SEQ ID NO: 1 with mutations P38Y, P53S, E90Y, Q129L and M136N; (uuu) SEQ ID NO: 1 with mutations P38Y, D47N, P53S, E90Y and Q129L; (vvv) SEQ ID NO: 1 with mutations P38Y, D47N, E52R, Q129L and M136N; (www) SEQ ID NO: 1 with mutations P38Y, D47N, H54R, E90Y, Q129L and M136N; (xxx) SEQ ID NO: 1 with mutations P38Q, E52R and E90Y; (yyy) SEQ ID NO: 1 with mutations D47N, E90Y, Q129L and M136N; (zzz) SEQ ID NO: 1 with mutations D47N, P53S, H54R, Q129L and M136N; (aaaa) SEQ ID NO: 1 with mutations D47N, E52R, P53T, H54R, E90Y, Q129L and M136N: (bbbb) SEQ ID NO: 1 with mutations E52R and E90Y; (cccc) SEQ ID NO: 1 with mutations E52R and H54R; (dddd) SEQ ID NO: 1 with mutations E52R, H54R, E90Y and M136N; (eeee) SEQ ID NO: 1 with mutations E52R, H54R, E90Y, Q129L and M136N; (ffff) SEQ ID NO: 1 with mutations P53S, H54R, E90Y, Q129L and M136N; and (gggg) SEQ ID NO: 1 with mutations E90Y Q129L and M136N.