Patent ID: 7862831
Filing Date: 2011-01-04
Classification: A61K,A61L,A61P,C12N

Abstract:
1. A method of integrating a hydrogel to the extracellular matrix of a musculoskeletal tissue, comprising the steps of: (a) priming the surface of a musculoskeletal tissue by treating the musculoskeletal tissue with a priming agent to create a primed musculoskeletal tissue, wherein the priming agent comprises a polysaccharide with at least one produced aldehyde group and a first at least one free radical polymerizable group, wherein the aldehyde is produced by treating said polysaccharide with periodate, and the least one produced aldehyde group reacts with the extracellular matrix of the musculoskeletal tissue to covalently bind the extracellular matrix of the musculoskeletal tissue and the priming agent; (b) adding to the primed musculoskeletal tissue a polymerizable agent and a radical photoinitiator, wherein the polymerizable agent comprises a biocompatible polymer having second at least one free radical polymerizable group; and (c) reacting the primed musculoskeletal tissue and the polymerizable agent by a radical reaction to create an integrated hydrogel covalently bound to the musculoskeletal tissue; wherein the radical reaction is between the first and second free radical polymerizable groups, and the first and second free radical polymerizable groups are each independently selected from the group consisting of an acrylate, diacrylate, oligoacrylate, methacrylate, dimethacrylate, and oligo methacrylate.