Patent ID: 6743435
Filing Date: 2004-06-01
Classification: A61K

Abstract:
A method for processing dermal tissue for implantation into a subject, said method comprising the steps of:a. removing the epidermal layer of said dermal tissue to produce de-epidermalized tissue; b. incubating said de-epidermalized tissue in at least one processing solution to remove cells from said de-epidermalized tissue, thereby producing a decellularized tissue matrix; c. treating said decellularized tissue matrix to cause a reduction in size and an increase in surface area; and d. exposing said treated decellularized tissue matrix to an acylating agent for a time ranging from about 30 seconds to about 10 minutes, wherein the amount of said acylating agent is about 0.1% to about 0.3% of wet tissue weight, thereby producing a dispersed tissue matrix.