Patent ID: 7914994
Filing Date: 2011-03-29
Classification: B01D,B01L,C12M,C12N,G01N,Y10T

Abstract:
1. A method for extracting nucleic acid from a sample, the sample containing cells, spores, or microorganisms, the method comprising: a) introducing the sample into a cartridge having: b) drying the sample on paper or membrane material in the lysing region, the paper or membrane material being impregnated with at least one chemical for lysing the cells, spores, or microorganisms in the sample to release the nucleic acid from the cells, spores, or microorganisms, while binding contaminants or inhibitors in the sample to the paper or membrane material; c) placing fluid in the lysing region and releasing the nucleic acid from the paper or membrane material into the fluid while the contaminants or inhibitors remain bound to the paper or membrane material; d) forcing the fluid containing the nucleic acid to flow through the capture region to capture the nucleic acid with the capture material; and e) eluting the nucleic acid from the capture region.