Patent ID: 8008045
Filing Date: 2011-08-30
Classification: C12Q

Abstract:
1. A method for detecting hepatitis C virus, comprising the steps of: subjecting a clinical sample to isothermal amplification with at least one pair of primers selected from the following pairs of primers (1) to (5); and determining a subtype of hepatitis C virus based on the resulting amplified product: (1) a pair of primers comprising at least eighteen consecutive bases corresponding to a 3′ end region of the base sequences of SEQ ID NOs: 1 and 2, respectively; (2) a pair of primers comprising at least eighteen consecutive bases corresponding to a 3′ end region of the base sequences of SEQ ID NOs: 3 and 4, respectively, or a pair of primers comprising at least eighteen consecutive bases corresponding to a 3′ end region of the base sequences of SEQ ID NOs: 3 and 9, respectively; (3) a pair of primers comprising at least eighteen consecutive bases corresponding to a 3′ end region of the base sequences of SEQ ID NOs: 5 and 6, respectively, having a property of being capable of genotyping hepatitis C virus subtype 2a gene, or a pair of primers comprising at least eighteen consecutive bases corresponding to a 3′ end region of the base sequences of SEQ ID NOs: 5 and 10, respectively; (4) a pair of primers comprising at least eighteen consecutive bases corresponding to a 3′ end region of the base sequences of SEQ ID NOs: 7 and 8, respectively; and (5) a pair of primers comprising at least eighteen consecutive bases corresponding to a 3′ end region of the base sequences of SEQ ID NOs: 21 and 22, respectively.