Patent ID: 6248579
Filing Date: 2001-06-19
Classification: C12N,C12P

Abstract:
An isolated polynucleotide molecule comprising the nucleotide sequence of the Streptomyces avermitilis aveC allele or the AveC gene product-encoding sequence of plasmid pSE186 (ATCC 209604) or the nucleotide sequence of the aveC ORF of S. avermitilis as presented in FIG. 1 (SEQ ID NO:1), but which nucleotide sequence further comprises one or more mutations such that cells of S. avermitilis strain ATCC 53692 in which the wild-type aveC allele has been inactivated and that express the polynucleotide molecule comprising the mutated nucleotide sequence produce a reduced class 2:1 ratio of avermectins than are produced by cells of S. avermitilis strain ATCC 53692 that instead express only the wild-type aveC allele.