Patent ID: 6908617
Filing Date: 2005-06-21
Classification: C07K,C12N

Abstract:
1. A modified gp 120 polypeptide comprising modifications in at least two conserved regions of an envelope protein selected from a primate lentivirus, wherein at least two of the four glycosylation sites in theses regions proximal to the CD4 binding site or CCR5 or CXCR4 chemokine receptor binding site have been altered, wherein the glycosylation sites are selected from the group of amino acids that correspond to positions 197, 276, 301 and 386 of HIV-1 strain HXBc2, wherein the alteration prevents glycosylation at said sites, and wherein the modified polypeptide maintains the overall 3-dimensional structure of a discontinuous conserved epitope of the wild-type gp 120, wherein the discontinuous conserved epitope is a CD4bs epitope or a CD4i epitope, and wherein the gp 120 protein is selected from the group consisting of HIV-1, HIV-2 and SIV.