Patent ID: 6154707
Filing Date: 2000-11-28
Classification: C12Q

Abstract:
Computer executable logic for use in genotyping a test sample of DNA at at least two allelic sites by comparing fluorescence spectra of control samples representing DNA having different combinations of the two alleles to a fluorescence spectrum of the test sample, the fluorescence spectra of the test and control samples being taken after the test and control samples have undergone a 5' nuclease assay in the presence of allelic probes for the at least two allelic sites, the computer executable logic comprising:logic for calculating fluorescence contributions of each fluorescer to a fluorescence spectrum where the fluorescence contributions for each fluorescer is based on fluorescence measurements at a series of wavelengths;logic for normalizing the calculated fluorescence contributions of each fluorescer to the fluorescence spectrum relative to an internal standard; andlogic for selecting which of the control samples has normalized fluorescence contributions associated with its control spectrum that matches the normalized fluorescence contributions of the test sample.