Patent ID: 7038193
Filing Date: 2006-05-02
Classification: G01J,G02B

Abstract:
1. A method for separating different emission wavelengths in a scanning microscope, comprising: scanning a specimen with an illuminating light beam by passing the illuminating light beam over the specimen using a beam deflector; selectively applying each of a plurality of excitation wavelengths to the illuminating light beam during the scanning according to a predefinable illumination scheme, wherein the selectively applying is performed so as to vary at least one of a light intensity and a time duration of an application of at least one of the excitation wavelengths over a time period of the scanning; detecting emission light coming from the specimen using at least one detector, the emission light including a plurality of emission wavelengths corresponding to the excitation wavelengths, the at least one detector being read out upon each selective applying of a respective excitation wavelength so as to provide respective corresponding detected signals; associating the detected signals with the respective excitation wavelengths using the illumination scheme; and specifying the at least one of the variation in the light intensity and the time duration as a function of information obtained from data of at least one of a momentary image and a preceding image, the information being extracted using an image processing.