Patent ID: 7488577
Filing Date: 2009-02-10
Classification: C12Q,G01N

Abstract:
1. A method for measuring DNA-dependent DNA polymerisation in a biological sample, comprising the steps of a) providing a primer with a single stranded short specific sequence, which is unable to base pair internally, bound to a solid phase, b) contacting the solid phase bound primer with a reaction mixture containing the four deoxynucleoside triphosphates, one of which is modified so that it is specifically recognized by a labeled antibody, a single stranded deoxynucleotide template construct composed of, from the 5′ end, a (A)n polymer and a variable part (CTGA)m or (CTG)m, and a sequence complementary to the primer, and n is greater than zero and m is greater than zero, with the proviso that n may be zero when the template is (CTGA)m, c) adding a biological sample comprising the DNA polymerase to the mixture of b), d) allowing the polymerase reaction to proceed, e) incubating the immobilized reaction product resulting from d) with the labeled antibody, f) detecting the amount of bound labeled antibody with the aid of the label used, and g) measuring the amount of incorporated modified deoxynucleoside triphosphate, as a measure of the DNA polymerization, with the aid of the label of the bound antibody.