Patent ID: 6818395
Filing Date: 2004-11-16
Classification: B01L,C12Q,F04B,F15C,F16K,G01N

Abstract:
A method of analyzing a target polynucleotide comprising:(a) pretreating the surface of a substrate with a polyelectrolyte multilayer (PEM) to create surface chemistry that facilitates polynucleotide attachment and sequence analysis; (b) providing a primed target polynucleotide attached to a surface of a substrate; (c) providing a labeled first nucleotides to the attached target polynucleotide under conditions whereby the labeled first nucleotide attaches to the primer, if a complementary nucleotide is present to serve as template in the target polynucleotide; (d) determining presence or absence of a signal, the presence of a signal indicating that the labeled first nucleotide was incorporated into the primer, and hence the identity of the complementary base that served as a template in the target polynucleotide; (e) repeating steps (c)-(d) with a labeled further nucleotide, the same or different from the first labeled nucleotide, whereby the labeled further nucleotide attaches to the primer or a nucleotide previously incorporated into the primer; and (f) repeating step (e) until identities of the bases in a portion or all of the target polynucleotide are determined.