Patent ID: 6130062
Filing Date: 2000-10-10
Classification: A01K,A61K,A61P,C07K

Abstract:
A method of increasing production of mammalian CD46 protein in a host cell, wherein a nucleic acid encoding the CD46 protein has an A and/or T rich region containing more than 60% A and/or T in an exon, said method comprising the steps of:altering the nucleic acid by reducing the amount of A and/or T in said region to less than 60% A and/or T;transfecting a host cell with said altered nucleic acid, andobtaining expression of said altered nucleic acid under appropriate conditions,wherein the level of CD46 protein production resulting from expression of said altered nucleic acid is increased relative to the level of CD46 protein production resulting from expression of said nucleic acid before alteration; andwherein RNA stability of RNA produced from expression of said altered nucleic acid is not demonstrably altered relative to the stability of RNA produced from expression of said nucleic acid before alteration.