Patent ID: 6482794
Filing Date: 2002-11-19
Classification: C12P,Y10S

Abstract:
A process for augmenting, enhancing or imparting an aroma in or to a consumable material selected from the group consisting of perfumed articles and perfume compositions comprising the steps of intimately admixing (a) an aroma augmenting, enhancing or imparting quantity and concentration of an acetophenone-containing product produced according to the process for the high yield production of acetophenone comprising the steps of:i. preparing an aqueous nutrient medium as a first aqueous liquid phase, containing a source of carbon, a nitrogen source, a buffer and yeast extract at a neutral pH of about 7; ii. inoculating said first aqueous liquid phase with the bacterial species, Comamonas testosteroni ATCC 17409, and incubating said first aqueous liquid phase for a sufficient period of time with agitation to produce a grown culture of said bacteria species; iii. preparing an acetophenone production medium which is a mixture containing a carbon source, a nitrogen source, a buffer and yeast extract at a neutral pH of about 7; iv. mixing said grown culture and said acetophenone production medium together with agitation to form a reaction medium; v. feeding a source of cinnamic acid to said reaction medium at a rate sufficient to maintain a concentration in the range of 5-10 grams per liter of said cinnamic acid source at a rate sufficient to enable said bacteria species to maintain oxidative growth; and vi. aerating with an oxygen-containing gas at a rate enabling interaction with said carbon source to avoid production of unwanted alcohol in a sufficient amount to maintain oxidative conditions throughout the reaction to thereby achieve an oxidation reaction in the presence of said bacteria species capable of producing said natural acetophenone compound in a high yield, with (b) a perfume base or a perfumed article base, said acetophenone-containing product being defined according to the mass spectrum of FIG. 1.