Patent ID: 9133526
Filing Date: 2015-09-15
Classification: C12Q

Abstract:
1. A method of detecting a strain of Clostridium difficile in a sample, the method comprising: hybridizing a target nucleic acid sequence from the sample with at least one primer set selected from (a) a primer set comprising (i) a primer comprising SEQ ID NO: 1 and (ii) a primer comprising SEQ ID NO: 2; and (b) a primer set comprising (i) a primer comprising SEQ ID NO: 4 and (ii) a primer comprising SEQ ID NO: 5; hybridizing a target nucleic acid sequence from the sample with at least one primer set selected from (a) a primer set comprising (i) a primer comprising of SEQ ID NO: 7 and (ii) a primer comprising SEQ ID NO: 8; (b) a primer set comprising (i) a primer comprising of SEQ ID NO: 22 and (ii) a primer comprising SEQ ID NO: 23; and (c) a primer set comprising (i) a primer comprising of SEQ ID NO: 25 and (ii) a primer comprising SEQ ID NO: 26; amplifying the target nucleic acid sequence; and detecting the amplified target nucleic acid sequence; wherein detection of the amplified target nucleic acid sequence indicates the presence of a strain of