Patent ID: 7510834
Filing Date: 2009-03-31
Classification: C12Q

Abstract:
1. A method of identifying a DNA sequence fragment comprising a microsatellite in a human genomic region in which a gene associated with a phenotype exists, comprising: selecting a combination of DNA sequences comprising SEQ ID Nos. 1-27088, wherein each of the sequences comprises a microsatellite genetic polymorphism marker; collecting DNA samples from subjects affected with said phenotype and control subjects not affected with said phenotype; performing polymerase chain reactions (PCR) on the DNA samples using forward primers consisting of 15-25 nucleotides wherein the forward primers consist of the same nucleotide sequence as the sequence extending in the 3′-direction from the 5′-terminus of each of the DNA sequences in said combination and reverse primers consisting of 15-25 nucleotides wherein the reverse primers consist of the sequence complementary to the sequence extending in the 5′-direction from the 3′-terminus of each of the DNA sequences in said combination to produce DNA sequence fragments, wherein each of said DNA sequence fragments comprises a microsatellite genetic polymorphism marker; analyzing alleles of the microsatellite genetic polymorphism markers of said DNA sequence fragments; and statistically comparing allele frequencies observed in the DNA sequence fragments produced from the affected subjects with those observed in the DNA sequence fragments produced from the control subjects to identify microsatellite polymorphism markers found positive whose allele frequencies observed in the DNA sequence fragments produced from the affected subjects are statistically significantly different from the allele frequencies observed in their corresponding DNA sequence fragments produced from the control subjects, wherein the DNA fragments comprising at least one microsatellite polymorphism marker found positive are in a human genomic region in which a gene associated with a phenotype exists.