Patent ID: 6355412
Filing Date: 2002-03-12
Classification: C12N,C12Q

Abstract:
A method for introducing a double-stranded target DNA into a vector comprising culturing a bacterial cell that expresses a functional recombinase, said bacterial cell containing (a) the target DNA comprising a first double-stranded terminus and a second double-stranded terminus, and (b) a vector DNA comprising, in the following order along the vector DNA strand: (i) a first double-stranded homology arm; (ii) an origin of replication; and (iii) a second double-stranded homology arm, such that the sequence of a vector DNA strand of the first homology arm is designed to be homologous to the sequence of a target DNA strand of the first terminus, and the sequence of a vector DNA strand of the second homology arm is designed to be homologous to the sequence of the target DNA strand of the second terminus, such that the target DNA is inserted into the vector DNA between the homology arms.