Patent ID: 9125357
Filing Date: 2015-09-08
Classification: C12N,G06Q

Abstract:
1. A method to produce a predetermined level of a transgenic trait in a harvested crop comprising: a) measuring expression level of the transgenic trait in a first variety of seeds; b) determining the yield potential of said first variety of seeds; c) determining the yield potential of a second variety of seeds; d) calculating a blend ratio of the first variety of seeds to the second variety of seeds that will produce the predetermined level of the transgenic trait in the harvested crop, wherein the predetermined level of the trait in the harvested crop, the expression level of a trait in the first variety of seeds, yield potential of said first variety of seeds and the yield potential of said second variety of seeds are used to determine the blend ratio of the first variety of seeds to the second variety of seeds; wherein the calculating is performed using the equation E(A)=(X e) blending the first variety of seeds with the second variety of seeds to the blend ratio calculated in step d); f) planting the blend of seeds; and g) harvesting the crop with the predetermined level of the transgenic trait, wherein said transgenic trait of interest is an enzyme, wherein said enzyme is an amylase.