Patent ID: 9164058
Filing Date: 2015-10-20
Classification: B01D,B65D,C07K,C08F,C08K,G01N

Abstract:
1. A method of making a polyacrylamide gel, the method comprising: providing a first solution comprising acrylamide/bis-acrylamide and a second solution comprising triethanolamine, an ampholyte ampholyte selected from the group consisting of glycine and tricine, and a conjugate ampholyte consisting of an amino acid with a pKa within the range of 8.3 to 9.6, wherein the first solution and second solution are stored separately for at least 7 days, then forming a mixture of the first solution and the second solution and a polymerization catalyst; adding said mixture into the mold; and incubating the mixture in the mold under conditions sufficient to generate the polyacrylamide gel.