Patent ID: 6767561
Filing Date: 2004-07-27
Classification: A61K,A61P,Y02A

Abstract:
A process for obtaining a dry extract of Mikania micrantha, Mikania scandens and Mikania cordata leaves, comprising at least 50% by weight of mikanolide and dihydromikanolide and at least 20% dihydromikanolide, comprisinggrinding and drying Mikania micrantha, Mikania scandens and Mikania cordata leaves; adding to the ground leaves of Mikania micrantha, Mikania scandens and Mikania cordata leaves, a solvent selected from the group consisting of toluene, ethyl acetate, toluene and ethyl acetate mixtures, toluene and acetone mixtures, ethyl acetate and heptane mixtures, and heptane and acetone of the mixtures in proportions of 7:3 to 3:7; filtering the mixture and recovering the filtrate; concentrating the filtrate of the primary extract solution to obtain a concentration of 2.5 to 10% dry extract; dividing of the concentrated extract obtained in the preceding stage between the extraction solvent and a mixture comprising from 10% to 50% methanol or ethanol in water; washing of the alcohol-water phase with n-hexane or heptane; eliminating the alcohol of the alcohol-water phase; extracting the aqueous phase obtained with ethyl acetate; drying of the ethyl acetate phase with a drying agent; evaporating the solvents and recovering the dry extract; purifying the dry extract obtained by a physico-chemical separation method; obtaining, after elimination of the solvents, the desired extract.