Patent ID: 7267837
Filing Date: 2007-09-11
Classification: C12Q

Abstract:
1. A process for the preparation of enzyme electrode useful for estimation of cholesterol in aqueous medium, which comprises the steps of: a. preparing a silicate solution, b. immobilizing an enzyme cholesterol oxidase and an electron mediator by slowly adding 0.05-0.1 M phosphate buffer containing 3-5 IU of cholesterol oxidase and about 0.01 M of electron mediator to the above said silicate solution of step a), c. allowing the resultant mixture to stand till the complete encapsulation of enzyme and mediator by observing turbidity, d. spreading the resultant turbid mixture on an electrically conductive base plate, e. drying the electrically conductive base plate with the spread mixture for at least one day at a temperature in a range of 25-30° C. to obtain the enzyme electrode comprising: