Patent ID: 7368245
Filing Date: 2008-05-06
Classification: C12Q

Abstract:
1. A method of determining the presence of chromosome aberrations, wherein said aberrations are selected from deletions, amplifications, inversions or aneuploidy in a chromosome in a sample of eukaryotic origin, using in situ hybridisation, comprising the steps of a) contacting said sample with a hybridisation solution comprising at least two sets of hybridisation probes, wherein at least one set comprises one or more probes capable of hybridising to specific nucleic acid sequences related to a potential aberration in a chromosome, and at least one set comprises one or more probes capable of hybridising to specific nucleic acid sequences related to another or the same potential aberration in a chromosome, wherein at least one set of hybridisation probes is a set of peptide nucleic acid probes, and wherein each probe is labelled directly or indirectly with at least one detectable label, wherein the detectable label of the at least one set of hybridisation probes is different from the detectable label of the at least one other set of hybridisation probes and optionally a hybrid destabilising agent in an amount effective to decrease the melting temperature of hybrids formed between said nucleic acid sequences and said peptide nucleic acid probes so as to increase the ratio between specific and non-specific binding, b) removing unbound and non-specifically bound peptide nucleic acid probe, and c) determining the presence of the bound peptide nucleic acid probe in the preparation.