Patent ID: 9096648
Filing Date: 2015-08-04
Classification: B01D,B01J,C07K

Abstract:
1. A flow-through process for purifying a target molecule from a Protein A eluate comprising the steps of: (i) contacting the eluate recovered from a Protein A chromatography column with activated carbon, thereby to remove host cell proteins (HCPs); (ii) contacting the flow-through sample from step (i) with an anion exchange chromatography media; (iii) contacting the flow-through sample from step (ii) with a cation exchange chromatography media; and (iv) obtaining the flow-through sample from step (iii) comprising the target molecule, wherein the eluate flows continuously through steps (i)-(iii) and wherein level of HCPs in the flow-through sample after step (i) is less than 30% of the level in the eluate prior to step (i).