Patent ID: 7041446
Filing Date: 2006-05-09
Classification: C07H,C07K

Abstract:
1. A method for analyzing a function of a protein, comprising the steps of: i) producing a labeled protein which consists of a protein portion and a labeling compound chemically linked to a C-terminal of the protein portion by the steps of adding a nucleic acid containing a gene to a cell-free protein synthesis system as a template, and performing protein synthesis in the presence of the labeling compound to produce the labeled protein consisting of the protein portion and the labeling compound chemically linked to the C-terminal of the protein portion, wherein the labeling compound consists of a) an acceptor portion and b) a non-radioactive label substance chemically linked to the acceptor portion, wherein said acceptor portion comprises one member selected from the group consisting of puromycin, a puromycin derivative having the ability to bind to the C-terminal of a synthesized protein when protein synthesis is performed in a cell-free protein synthesis system or in a living cell, 3′-N-aminoacylpuromycin aminonucleoside and 3′-N-aminoacyladenine aminonucleoside, wherein said labeling compound is present at a concentration effective for said labeling compound to bind to the C-terminal of the synthesized protein, said protein being translated in the presence of said labeling compound; ii) contacting the labeled protein with a target molecule; and iii) determining an interaction between the labeled protein and the target molecule, to thereby analyze a function of the labeled protein.