Patent ID: 8114648
Filing Date: 2012-02-14
Classification: A61P,C07K,C12N,C12P

Abstract:
1. A method for producing an antibody that specifically binds to a polypeptide having one or more unnatural amino acids, wherein said method comprises: providing a translation system with at least one nucleic acid comprising at least one selector codon, wherein the nucleic acid encodes the polypeptide, and wherein the translation system translates the selector codon with the unnatural amino acid with a fidelity greater than about 75%; providing the translation system with an orthogonal tRNA (OtRNA), wherein the OtRNA recognizes the at least one selector codon; providing the translation system with an orthogonal aminoacyl tRNA synthetase (ORS), wherein the ORS preferentially aminoacylates the OtRNA with the one or more unnatural amino acids in the translation system, and wherein a K providing the translation system with the one or more unnatural amino acids, thereby producing in the translation system said polypeptide comprising the one or more unnatural amino acids; and, immunizing a subject with a sufficient amount of said polypeptide to produce said antibody.