Patent ID: 6790662
Filing Date: 2004-09-14
Classification: C07K,G01N

Abstract:
A method of isolating human CD8+ cells which comprises the steps of:(a) contacting a sample of isolated peripheral mononuclear blood cells with a first antibody which specifically binds to an epitope comprising AAEGLDTQRFSG (SEQ ID NO:1), on CD8 molecules present on the surface of human CD8+ cells but does not activate the CD8+ cells once bound thereto, under conditions permitting the formation of a first complex between the CD8+ cell and the first antibody; (b) separating from the sample any first antibody not present in the first complex; (c) contacting the sample with an immobilized second antibody which specifically binds to the first antibody in the first complex, under conditions permitting the formation of an immobilized second complex between the first complex and the second antibody, thereby immobilizing the CD8+ cells present in the sample; (d) separating the immobilized second complex from the sample; (e) contacting the immobilized second complex with an agent, which under suitable conditions causes dissociation of the second complex into CD8+ cells and an immobilized third complex between the first antibody and second antibody; and (f) separating the immobilized third complex from the CD8+ cells, thereby isolating the CD8+ cells.