Patent ID: 8546136
Filing Date: 2013-10-01
Classification: C12N,C40B

Abstract:
1. A library of nucleic acid molecules comprising at least one of each of the following nucleic acid molecules: (a) a first nucleic acid molecule wherein the first nucleic acid molecule is circular and comprises, in a 5′ to 3′ orientation, a first restriction site RA (b) an intermediate nucleic acid molecule wherein the intermediate nucleic acid molecule n is circular and comprises, in a 5′ to 3′ orientation, a first restriction site RA (c) a last nucleic acid molecule wherein the last nucleic acid molecule is circular and comprises, in a 5′ to 3′ orientation, a first restriction site RA whereupon cleavage of restriction sites RA 0 through RB m , and denaturation of the resulting linear nucleic acid molecules, each annealable linker sequence LB (p-1) is capable of hybridizing to the complement of annealable linker sequence LA p , wherein n is an integer that varies from 1 to (m−1), wherein p represents an integer from 1 to m, and wherein each group D 0 , . . . D n , . . . and D m independently consists of one or more DNA segments.