Patent ID: 6210914
Filing Date: 2001-04-03
Classification: C07K,C12N

Abstract:
A method for isolating a polynucleotide encoding a protein that binds to integrin regulatory protein 1(IRP-1) which is encoded by a DNA selected from the group consisting of the human IRP-1 DNA set out in SEQ ID NO: 1; and a DNA molecule which hybridizes under stringent conditions to a polynucleotide complementary to the protein coding portion of SEQ ID NO: 1, said stringent conditions including a final wash at 65.degree. C. in 0.2.times.SSC or the equivalent; said method comprising the steps of:a) transforming or transfecting host cells with a DNA construct comprising a reporter gene under the control of a promoter regulated by a transcription factor having a DNA-binding domain and an activating domain; said host cells being capable of expressing said reporter gene;b) expressing in said host cells a first hybrid DNA sequence encoding a first fusion of part or all of IRP-1 to either the DNA binding domain or the activating domain of said transcription factor;c) expressing in said host cells a library of second hybrid DNA sequences wherein each of said second hybrid sequences encodes a second fusion of part or all of a putative IRP-1 binding protein to the DNA binding domain or activating domain of said transcription factor which is not incorporated in said first fusion;d) detecting binding of an IRP-1 binding protein to IRP-1 in said host cells by detecting the production of reporter gene product in said host cell; ande) isolating a DNA sequence encoding an IRP-1 binding protein from said host cells in which reporter gene expression is detected.