Patent ID: 7375259
Filing Date: 2008-05-20
Classification: C12N,Y02A

Abstract:
1. An isolated nucleic acid molecule encoding a SGT2 polypeptide having UDP-glucose:solanidine glucosyltransferase activity, wherein said nucleic acid molecule is selected from the group consisting of: (a) a nucleic acid molecule with polypeptide coding sequence having at least 99% nucleotide sequence identity with SEQ ID NO:1 [from nucleotide 7 to nucleotide 1521, with SEQ ID NO:3 or with SEQ ID NO:5 from nucleotide 19 to nucleotide 1011 ]; (b) a nucleic acid sequence which encodes a polypeptide having at least 99% identity with SEQ ID NO:2 [or SEQ ID NO:4]; (c) a nucleic acid sequence which hybridizes under high stringency conditions with SEQ ID NO:1 [from nucleotide 7 to nucleotide 1521, with SEQ ID NO:3 or with SEQ ID NO:5 from nucleotide 19 to nucleotide 1011], wherein said high stringency conditions comprise hybridization at 68° C. in a solution consisting of 5X SSPE, 1% SDS, 5X Denhardt's reagent and 100 μg/ml denatured salmon sperm DNA followed by washing in a solution comprising 0.1X SSPE, and 0.1% SDS at 68° C.; (d) a nucleic acid molecule as shown in SEQ ID NO:1 [, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:14 or SEQ ID NO:16]; (e) an RNA equivalent of the sequences of (a), (b), (c), or (d); and (f) a full-length complement of the molecule defined in (a), (b), (c), (d) or (e).