Patent ID: 8592143
Filing Date: 2013-11-26
Classification: A61P,C07K,C12N,C12Q

Abstract:
1. A method for determining whether a hepatitis B virus (“test virus”) from a human patient would exhibit reduced sensitivity to a substituted acyclic nucleoside phosphide selected from adefovir and tenofovir, the method comprising: screening a nucleic acid molecule from the test virus for an adefovir- or tenofovir-resistant co-mutation at the codon encoding amino acid position 181 from alanine to threonine or valine and the codon encoding amino acid position 236 from asparagine to threonine where the amino acid position is numbered with reference to the methionine in the motif YMDD being residue 204, wherein the nucleic acid molecule comprising a nucleic acid sequence that encodes a reverse transcriptase domain of a DNA polymerase; and; wherein the mutation indicates that the test virus would exhibit reduced sensitivity to the substituted acyclic nucleoside phosphate.