Patent ID: 6054436
Filing Date: 2000-04-25
Classification: A01K,A61K,A61P,C07D,C07F,C07H,C07K,C12N,C12P

Abstract:
A method for selectively killing a hematopoietic cell which has been genetically engineered ex vivo and introduced into an animal, the method comprising administering to the animal an effective amount of a ligand to induce apoptosis of the hematopoietic cell, wherein the cell comprises at least one genetic construct encoding a chimeric protein which comprises:(a) at least one ligand-binding domain which binds to the ligand to form a ligand cross-linked protein complex including the chimeric protein, the ligand having one or more of the following characteristics:(i) the ligand is not a protein,(ii) the ligand has a molecular weight less than 5 kD, and(iii) the ligand is membrane permeable; and(b) an action domain, which is heterologous with respect to said at least one ligand-binding domain, and which induces apoptosis in the hematopoietic cell as a result of formation of the ligand cross-linked protein complex.