Patent ID: 8716031
Filing Date: 2014-05-06
Classification: C07K,C12N,G01N,Y10S,Y10T

Abstract:
1. A method of quantifying an antigen-specific canine IgE, comprising the following steps (A) to (D): (A) a step for contacting a biological sample from a subject dog with an antigen to bind IgE in the biological sample, whereby a complex of the IgE and the antigen is formed, (B) a step for measuring IgE in a standard sample of a mouse or rat, sensitized with the antigen of the step (A), by contacting the standard sample with the antigen to bind the IgE in the standard sample and with a rat or mouse monoclonal antibody, respectively, that recognizes canine IgE and also recognizes the mouse or rat IgE with equal affinity, wherein the monoclonal antibody does not recognize immunoglobulins other than IgE, to detect the bound standard IgE, and generating a standard curve, (C) a step for detecting an amount of the complex formed in the step (A) by contacting the complex with the same monoclonal antibody used in the step (B), and (D) a step for quantifying an amount of antigen-specific canine IgE in the biological sample from the subject dog by comparing the amount of complex detected in the step (C) and the standard curve generated in the step (B).