Patent ID: 7915025
Filing Date: 2011-03-29
Classification: A61P,C07K,C12N,C12P

Abstract:
1. A translation system, comprising: a nucleic acid comprising a selector codon, wherein the nucleic acid is homologous to a nucleic acid encoding an insulin-like growth factor (IGF); an unnatural amino acid, wherein the unnatural amino acid is a tyrosine analogue or a phenylalanine analogue; a tyrosyl orthogonal tRNA (OtRNA) that recognizes the selector codon; and, an orthogonal aminoacyl tRNA synthetase (ORS) wherein the ORS is derived from a tyrosyl synthetase and wherein the ORS comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 35-66; wherein the ORS preferentially aminoacylates the OtRNA with the unnatural amino acid; wherein a K