Patent ID: 8052967
Filing Date: 2011-11-08
Classification: A61K,C12N

Abstract:
1. A method for increasing the stability and the packaging capacity in a packaging cell of a recombinant subgroup B adenovirus particle comprising an E1B-55K deficient subgroup B adenovirus genome, wherein the genome comprises a deletion in the E1-region such that the genome does not encode the functional E1B-55K gene product, the method comprising: expressing in the packaging cell elements necessary for production and assembly of the subgroup B adenovirus genome into recombinant adenovirus particles, wherein expressing the elements further comprises expressing the subgroup B pIX gene product from the E1B-55K deficient subgroup B adenovirus genome from operably linked expression sequences containing at least 150 and not more than 600 nucleotides of adenovirus sequences directly upstream of the pIX open reading frame of a wild-type subgroup B adenovirus inclusive of the E1B 55K coding sequence, wherein the expression of the pIX in the packaging cell increases the stability and packaging capacity of the recombinant subgroup B adenovirus particle.