Patent ID: 7582476
Filing Date: 2009-09-01
Classification: A61P,C07K

Abstract:
1. An artificial cell comprising: (i) a chromosome which comprises a reporter polynucleotide, wherein the reporter polynucleotide comprises an ERE, a TATA sequence and a reporter sequence heterologous to the ERE; and (ii) a mutant ERα which has an activity for transactivation of the reporter polynucleotide, wherein in the presence of a partial anti-estrogen and E2 the activity is higher than that of ERα encoded by SEQ ID NO:1 in the presence of the partial anti-estrogen and E2, or in the presence of a partial anti-estrogen the activity is higher than that of ERα encoded by SEQ ID NO:1 in the presence of the partial anti-estrogen, and wherein the mutant ERα has an amino acid sequence of an ERα comprising one or more substituted amino acids at one or more relative positions selected from 303, 309, 390, 396, 494 and 578, or two or more substituted amino acids at two or more relative positions selected from 303, 309, 390, 396, 415, 494, 531 and 578, wherein the relative positions are based on a homology alignment to an amino acid sequence shown in SEQ ID NO:1.