Patent ID: 9056895
Filing Date: 2015-06-16
Classification: C07K,G01N,Y10T

Abstract:
1. A method of eluting target ligand molecules bound to receptor molecules comprising: providing a sample solution containing target peptide ligand molecules bound to receptor molecules in the absence of immobilizing surfaces, where the receptor molecules are substantially larger than the target peptide ligand molecules; adding excess amounts of labeled ligand molecules to the sample to compete with the target peptide ligand molecules and bind to the receptor molecules in place of the target peptide ligand molecules, releasing the target peptide ligand molecules; and separating and eluting the released target peptide ligand molecules from the remaining receptor molecules bound to labeled ligand molecules based on the presence or absence of a label on the ligands in the sample solution using a combination of surface-free isolation, specific competition elution and dual epitope isolation.