Patent ID: 6955887
Filing Date: 2005-10-18
Classification: C07K,G01N

Abstract:
1. A method of screening for a compound that modulates sweet taste signaling in taste cells, the method comprising the steps of: (i) contacting the compound with a hetero-oligomeric taste transduction G-protein coupled receptor that responds to sweet taste stimuli; wherein said hetero-oligomeric receptor comprises a polypeptide that is encoded by a nucleic acid sequence that specifically hybridizes under stringent hybridization conditions to a human T1R2 nucleic acid comprising the nucleotide sequence of SEQ ID NO:3, and further comprises a polypeptide that is encoded is by a nucleic acid sequence that specifically hybridizes under stringent hybridization conditions to the human T1R3 nucleotide sequence of SEQ ID NO: 5; wherein stringent hybridization conditions comprise conducting the hybridization reaction at 42° C. in a solution comprising 50% formamide, 5×SSC, and 1% SDS and washing at 65° C. in a solution comprising 0.2×SSC and 0.1% SDS; and (ii) determining whether said compound binds to and/or affects the activity of said hetero-oligomeric sweet receptor.