Patent ID: 6270968
Filing Date: 2001-08-07
Classification: C12N,C12Q,C12Y

Abstract:
A method for providing a hybrid polypeptide having an activity of interest, comprising:i) performing PCR amplification using an uncharacterized DNA sample and oligonucleotide primers with homology to one or more known genes encoding a polypeptide exhibiting the activity of interest, to obtain one or more PCR products of unknown sequence;ii) linking the obtained PCR products of unknown sequence to a 5' structural gene sequence and a 3' structural gene sequence, wherein the 5' and 3' structural gene sequences are derived from one or more genes encoding a polypeptide exhibiting the activity of interest, to form hybrid DNA sequences;iii) expressing the hybrid DNA sequences; andiv) screening the expressed hybrid DNA sequences to identify a sequence encoding a polypeptide exhibiting the activity of interest or a related activity.