Patent ID: 8288118
Filing Date: 2012-10-16
Classification: C12N,G01N

Abstract:
1. A method of analyzing various surface chemistries for culturing a given cell line, comprising the steps of: i) providing a plasma polymerized surface having first and second regions, said first region comprising a first concentration of carboxylic acid groups on said plasma polymerized surface and said second region comprising a second concentration of carboxylic acid groups on said plasma polymerized surface, wherein said first and second concentrations are different; ii) culturing cells from said cell line on said plasma polymerized surface in each said region; iii) observing activity selected from the group consisting of alkaline phophatase activity of the cultured cells, cell growth of the cultured cells, attachment of the cultured cells, and cell spreading of the cultured cells in each said region; and iv) analyzing the activity of the cultured cells in each said region.