Patent ID: 8263364
Filing Date: 2012-09-11
Classification: C12Q,G01N

Abstract:
1. A method of nucleic acid sequencing, the method comprising the steps of: (a) hybridizing a template to a universal primer that comprises a homopolymer region to form a primer/template system; (b) on a solid phase, catalyzing incorporation of a nucleotide to said primer/template system with a polymerase by exposing said primer/template system to said polymerase and one or more types of nucleotides comprising an optically-detectable label, wherein said optically-detectable label is not attached to the 3′ hydroxyl of the sugar moiety of said nucleotide; (c) detecting incorporated nucleotide by the presence of said label; (d) removing said label from said incorporated nucleotide; (e) permitting steps (b) through (d) to occur multiple times so as to determine a nucleic acid sequence based upon said incorporated nucleotides.