Patent ID: 7037682
Filing Date: 2006-05-02
Classification: C12N,C12Q

Abstract:
1. A method for producing HBV DNA polymerase, comprising: providing a 5′ sense oligonucleotide comprising an SP6 promoter and an oligonucleotide sequence matching the 5′ end of the human HBV DNA polymerase coding region; providing a 3′ antisense oligonucleotide comprising an oligonucleotide sequence matching the 3′ end of the human HBV DNA polymerase non-coding region; contacting the 5′ oligonucleotide and 3′ oligonucleotide with a sample containing a DNA encoding human HBV DNA polymerase; performing PCR amplification under conditions that allow amplification of the DNA encoding human HBV DNA polymerase to produce PCR products; and transcribing and translating the PCR products in eukaryotic cell-free lysates to produce in vitro translated human HBV DNA polymerase.