Patent ID: 8021862
Filing Date: 2011-09-20
Classification: C07K,C12N

Abstract:
1. An improved process for obtaining high volumetric yields of Granulocyte Colony Stimulating Factor (G-CSF) comprising i) inoculating a fermenter with a suitable recombinant E. coli culture transformed with an expression vector comprising a nucleic acid sequence that encodes G-CSF and ii) culturing the recombinant E. coli through pre-production and production phase of fermentation to express the nucleic acid sequence encoding G-CSF in a culture broth wherein the culture broth comprises potassium (K) ions in a concentration range of 60 mM to 180 mM in combination with inorganic cations selected from sodium (Na) in a concentration range of 90 mM to 300 mM, and magnesium (Mg) in a concentration range of 150 mM to 250 mM.