Patent ID: 7169577
Filing Date: 2007-01-30
Classification: B01J,B01L,C12M,C40B,G01N

Abstract:
1. A method of screening cells for a desired biological activity comprising: providing a cell isolation device defining a plurality of cell isolation regions, each cell isolation region encompassing a recess and bioaffinity ligands, each of the cell isolation regions being sized to isolate about one to about five cells on a surface within said recess, and the cell isolation regions positioned to have a predetermined pitch with respect to one another; delivering the about one cell to about five cells to each of the cell isolation regions; providing a cell expansion device defining a plurality of wells wherein the position of the wells of the cell expansion device corresponds to the predetermined pitch of the cell isolation regions; placing said cell isolation device in direct contact with said cell expansion device, inverting said cell isolation device, and transferring the about one cell to about five cells from said surface of said cell isolation regions to the wells; allowing the about one cell to about five cells to proliferate and exhibit a desired biological activity in the wells; assaying the desired biological activity of the one cell to about five cells.