Patent ID: 6136570
Filing Date: 2000-10-24
Classification: A61K,C07K,C12N,Y02A

Abstract:
A method for producing a viable RNA virus comprising the steps of:(a) culturing a host transformed with a recombinant DNA molecule comprising a full-length or infectious RNA virus cDNA produced by a method comprising the steps of:(i) isolating the genomic RNA from an RNA source virus,(ii) employin RNA sequencing means to determine the nucleotide sequence of a portion of said isolated genomic RNA,(iii) employing cDNA synthesis means to produce a double-stranded cDNA from said isolated genomic RNA,(iv) employing DNA sequencing means to determine the nucleotide sequence of a portion of said cDNA, wherein said portion of said cDNA corresponds to said portion of said RNA sequenced in step (ii),(v) comparing said sequenced cDNA with said sequenced RNA to determine differences in nucleotide sequence, and(vi) altering said differences in said cDNA to produce a full-length or infectious RNA virus cDNA,said host being selected from the group consisting of bacteria, yeast and other fungi, insect cells and animal cells, under conditions which permit the production of viable RNA virus; and(b) harvesting said viable RNA virus from said host cell culture, wherein said virus is vaccine strain 3 poliovirus.