Patent ID: 6162607
Filing Date: 2000-12-19
Classification: G01N,Y10S

Abstract:
A method for measuring an amount of a constituent in a first specific class of lipoprotein in a biological sample containing, in addition, at least one other class of lipoprotein by two point-end assay by using an autoanalyzer, wherein said first specific class of lipoprotein is selected from the group consisting of the chylomicrons class, the class of very low density lipoproteins, the class of low density lipoproteins, and the class of high density lipoproteins, and said at least one other class of lipoprotein is at least one member selected from the same group as the first specific class of lipoprotein but is other than the first specific class of lipoprotein, said method for measuring comprises, in sequential steps:mixing the biological sample with a first reagent composition comprising an antibody which does not bind with the first specific class of lipoprotein but which binds with said at least one other class of lipoprotein to start an antibody binding reaction, said mixing creating a reaction and forming a first reaction mixture and said antibody substantially preventing constituents in said at least one other class of lipoprotein from participating in the reaction in the first reaction mixture;measuring an absorbance, OD.sub.1 of the first reaction mixture;mixing the first reaction mixture after the antibody binding reaction of said mixture has been sufficiently completed with a second reagent composition containing no antibody and comprising a reagent for measuring the constituent in said at least one other class of lipoprotein by an enzymatic method to start an oxidase reaction, said mixing creating another reaction and forming a second reaction mixture;measuring an absorbance, OD.sub.2, of the second reaction mixture;calculating a value, OD.sub.3, by using the following equation:OD.sub.3 =OD.sub.2 -OD.sub.1 'wherein the OD.sub.1 ' is a value obtained by correcting the OD.sub.1 value in accordance with the difference between a volume of the first reaction mixture and the second reaction mixture; anddetermining the amount of the constituent contained in said first specific class of lipoprotein from OD.sub.3 by comparing the OD.sub.3 value to a calibration curve.