Patent ID: 6403340
Filing Date: 2002-06-11
Classification: C12Q,Y10T

Abstract:
A method for synthesizing oligonucleotide products comprising the steps of:providing a circular nucleic acid template sequence having at least one cleavage site which, when rendered double-stranded by a complementary strand, further provides a cleavage site for at least one cleavage agent which cleaves only the complementary strand; hybridizing to the template sequence a target nucleic acid sequence to act as a primer nucleic acid sequence; treating the template sequence and hybridized primer sequence with at least one strand-displacing polymerizing agent and reagents under conditions necessary to effect the action of the polymerizing agent with the at least one cleavage agent; and producing a complementary strand hybridized to the template sequence which is cleaved at the at least one cleavage site with at least one cleavage agent which cleaves only the complementary strand, wherein the complementary strand is subsequently displaced as a single-stranded linear nucleic acid by the polymerizing agent.