Patent ID: 6174674
Filing Date: 2001-01-16
Classification: C07K,C12N,Y10S

Abstract:
A method for detecting a t(2;5) chromosomal rearrangement comprising:(a) hybridizing a first probe labeled with a first detection reagent to a sample comprising a human chromosome band 2p23 and a human chromosome band 5q35; wherein said first probe is one or more polynucleotide(s) that hybridize(s), using a solution of sheared human DNA, 50% formamide, 10% dextran sulfate, 2.times.SSC, pH 7, and an overnight incubation at 37.degree. C., to a region of human chromosome 5, wherein said human chromosome 5 comprises a wild type NPM gene, wherein part of said wild type NPM gene has a sequence encoding amino acid 112 up to, and including, amino acid 117 of SEQ ID NO 4, wherein said region of human chromosome 5 is centromeric to said sequence encoding amino acid 117 of SEQ ID NO 4, and wherein said region of human chromosome 5 comprises a portion of said wild type NPM gene;(b) hybridizing a differently labeled second probe labeled with a second detection reagent to said sample; wherein said second probe is one or more polynucleolide(s) that hybridize(s), using a solution of sheared human DNA, 50% formamide 10% dextran sulfate, 2.times.SSC, pH 7, and an overnight incubation at 37.degree. C., to a region of human chromosome 2, wherein said human chromosome 2 comprises a wild type ALK gene, wherein part of said wild type ALK gene has a sequence encoding amino acid 119 up to, and including, amino acid 124 of SEQ ID NO 4, and wherein said region of human chromosome 2 is telomeric to said sequence encoding amino acid 119 of SEQ ID NO 4;(c) detecting said first probe and said second probe by detecting said first detection reagent and said second detection reagent; and(d) determining the location of said first probe relative to said second probe, wherein the appearance of said first probe and said second probe as paired signals that localize to said human chromosome 5q35 band indicates the presence of a t(2;5) chromosomal rearrangement in said sample.