Patent ID: 7405282
Filing Date: 2008-07-29
Classification: C07H,C12N,C12Q

Abstract:
1. A kit for identifying a nucleotide sequence that modulates the activity of a transcriptional regulatory region comprising a library of plasmids for incorporation into a host cell population wherein the library is a collection of plasmids each plasmid having one or more non-redundant U-tags, and wherein a plasmid in the library comprises a first DNA construct and a second DNA construct, wherein: a) the first DNA construct comprises a multiple cloning site, a reporter sequence, and an mRNA stabilizing sequence; and, b) the first DNA construct further comprises one or more U-tags, wherein each U-tag is a short, random nucleotide sequence inserted into an untranslated region of the reporter sequence, and one or more intron sequences occurring within the first DNA construct; and c) the second DNA construct comprises a promoter active in a host cell operably linked to a multiple cloning site.