Patent ID: 6713248
Filing Date: 2004-03-30
Classification: C07K,C12N,C12Q,G01N

Abstract:
An in vitro homogenous method of detecting cleavage of &bgr;-amyloid precursor protein (&bgr;APP) by gamma-secretase, said method comprising detecting binding of a gamma-cleaved &bgr;APP fragment with a pair of fluorescent adducts, wherein a first fluorescent adduct binds specifically to the carboxy-terminal end of the gamma-cleaved &bgr;APP fragment with substantially no cross-reactivity to uncleaved &bgr;APP or to other types of gamma-cleaved &bgr;APP fragments, and wherein a second fluorescent adduct binds to the gamma-cleaved &bgr;APP fragment within an amino acid sequence which corresponds to amino acids 1-31 of &bgr;-amyloid peptide (A&bgr;), and wherein excitation of one of the fluorescent adducts provides a detectable transfer of fluorescent energy to the other fluorescent adduct.