Patent ID: 6670127
Filing Date: 2003-12-30
Classification: B01J,C12N,C40B

Abstract:
A method of assembling a double-stranded polynucleotide comprising:(a) selecting a partially double-stranded initiating oligonucleotide, wherein said initiating oligonucleotide comprises a at least one overhang; (b) contacting said partially double-stranded initiating oligonucleotide with a next most terminal double-stranded oligonucleotide, wherein said next most terminal double-stranded oligonucleotide is contiguous with the initiating oligonucleotide and comprises at least one overhang, and wherein at least one overhang of said next most terminal double-stranded oligonucleotide is complementary to at least one overhang of said initiating oligonucleotide; (c) repeating (b) to sequentially add the next most terminal double-stranded oligonucleotide to the extended initiating oligonucleotide, whereby said double-stranded polynucleotide is synthesized.