Patent ID: 7811771
Filing Date: 2010-10-12
Classification: C12Q,G01N

Abstract:
1. A method for determining the activity of a mannan-binding-lectin associated serine protease (MASP) in a sample, the method comprising the steps of: incubating the sample with a pro-urokinase comprising at its activation site the consensus sequence Arg/Leu/Gly-Yyy-Arg/Lys-Ile/Leu/Val-Zzz-Gly-Gly (SEQ ID NO:1), wherein Yyy can be any amino acid and Zzz is an aliphatic amino acid; measuring proteolytic activation of said pro-urokinase incubated with said sample; and determining MASP activity in the sample based on said proteolytic activation.