Patent ID: 7332337
Filing Date: 2008-02-19
Classification: A61K,C12N

Abstract:
1. A method of delivering a heterologous gene sequence linked to a promoter sequence into a T lymphocyte cell, a B-cell, a mast cell, or a combination thereof, each of said cells having a common binding receptor, comprising (a) contacting in vitro said cell with said chimeric adenoviral particle having increased tropism for said T lymphocyte cell, a B-cell, a mast cell, or a combination thereof, relative to an adenoviral particle comprising only native adenoviral sequences, said chimeric adenoviral particle comprising a viral protein coat and containing said heterologous gene sequence linked to a promoter sequence, (b) allowing said chimeric adenoviral particle to transduce in vitro said T lymphocyte cell, a B-cell, a mast cell, or a combination thereof.