Patent ID: 9181540
Filing Date: 2015-11-10
Classification: C07K,C12N,C12P,C12Y

Abstract:
1. A method for producing a desired antibody, comprising culturing a cell capable of high-yield production of β-alanine to produce the desired antibody; wherein the cell has been transformed by DNA encoding the desired antibody, wherein the cell has been transformed by DNA encoding cysteine sulfinic acid decarboxylase or a mutant thereof, wherein the mutant has the same enzyme activity as cysteine sulfinic acid decarboxylase, and wherein the DNA encoding the cysteine sulfinic acid decarboxylase is selected from the group consisting of: (a) a DNA encoding a polypeptide consisting of the amino acid sequence as shown in SEQ ID NO: 8; (b) a DNA encoding a polypeptide consisting of an amino acid sequence derived from the amino acid sequence as shown in SEQ ID NO: 8 by substitution, deletion and/or addition of 1-10 amino acid residues, wherein the polypeptide has cysteine sulfinic acid decarboxylase activity; (c) a DNA encoding a polypeptide having 98% or more amino acid sequence homology with the amino acid sequence as shown in SEQ ID NO: 2, wherein the polypeptide has cysteine sulfinic acid decarboxylase activity; (d) a DNA consisting of the nucleotide sequence as shown in SEQ ID NO: 7; and (e) a DNA that hybridizes to a DNA complementary to a DNA consisting of the nucleotide sequence as shown in SEQ ID NO: 7 under high stringent conditions of 65° C., 2×SSC and 0.1% SDS, wherein the DNA encodes a polypeptide having cysteine sulfinic acid decarboxylase activity.