Patent ID: 8962917
Filing Date: 2015-02-24
Classification: A23K,A23L,A23V,C12N,C12P,C12Y

Abstract:
1. A polynucleotide operably linked to a heterologous nucleic acid sequence comprising: (a) a nucleotide sequence encoding a polypeptide having delta-5 desaturase activity, wherein the polypeptide has an amino acid sequence of at least 90% amino acid identity, based on the Clustal W method of alignment provided by the MegAlign™ v6.1 program of the DNASTAR LASERGENE bioinformatics computing suite (default parameters: GAP PENALTY=20, GAP LENGTH PENALTY=0.2, Delay Divergen Seqs(%)=30, DNA Transition Weight=0.5, Protein Weight Matrix=Gonnet Series, DNA Weight Matrix=IUB), when compared to an amino acid sequence as set forth in SEQ ID NO:2; (b) a nucleotide sequence encoding a polypeptide having delta-5 desaturase activity, wherein the nucleotide sequence has at least 90% sequence identity, based on the Basic Local Alignment Search Tool (“BLASTN”) method of alignment provide by the National Center for Biotechnology Information (default parameters: short queries=automatically adjust parameters for short input sequences, expect threshold=10, and filter=low complexity regions), when compared to a nucleotide sequence as set forth in SEQ ID NO:1 or SEQ ID NO:3; or (c) a complement of the nucleotide sequence of (a) or (b), wherein the complement and the nucleotide sequence consist of the same number of nucleotides and are 100% complementary.