Patent ID: 7008770
Filing Date: 2006-03-07
Classification: C12Q

Abstract:
1. A method for the controllable conducting of complex PCR amplifications, said method comprising the steps of: a) performing PCR amplification with at least 50 primers of a first type (type 1) of different sequence, which are complementary to one of the strands of a random DNA sample, and which also contains a primer or a library of primers of a second type (type 2), which is complementary to the other strand of the DNA sample used, whereby the type-2 primers contain a first label (label 1), whereby amplified products are produced; b) hybridizing the amplified products to an oligomer array, which comprises oligonucleotides that hybridize to the primers utilized in the PCR reaction or to oligonucleotides that are complementary to the primers utilized in the PCR reaction; or hybridizing the amplified products to an oligomer array, which contains oligomers complementary to the primers utilized in the PCR reaction; c) determining the length of the amplified products bound to the array by a second label (label 2) which is correlated with the length of the respective DNA fragment, and which is different from the first label (label 1) in step a) and d) quantifying the signals originating from label 1 and label 2 at each site of the oligonucleotide array relevant for the analysis.