Patent ID: 6811974
Filing Date: 2004-11-02
Classification: C12Q

Abstract:
A primer specific and mispair extension assay for determining genotype, said assay comprising:a) extending a nucleic acid sequence from a patient sample with pfu DNA polymerase, using a primer specific for a genotype to be determined, and an incomplete set of dNTPs in the absence of ddNTPs, under suitable conditions for obtaining one or more extension products of the primer wherein said one or more extension products are terminated in the presence of at least two mispairs within a 2 to 4 base pair range located downstream of the 3â€² end of the primer; and wherein at least one of the primer or the dNTPs is labeled; b) characterizing the extension products; and c) analyzing the characterized extension products based on primer-specific pairing and non-specific pairing to determine the genotype of the nucleic acid sequence extended.