Patent ID: 7884086
Filing Date: 2011-02-08
Classification: C12N,C12Y

Abstract:
1. A method for reducing the level of target mRNA in a cell comprising: contacting the cell with a single-stranded sense oligonucleotide consisting of 10 to 40 linked nucleosides; and contacting the cell with a single-stranded antisense oligonucleotide consisting of 10 to 40 linked nucleosides at least one hour after contacting the cell with the sense oligonucleotide; wherein the antisense oligonucleotide is complementary to the target mRNA, the sense and antisense oligonucleotides are fully complementary to each other, the contacting occurs in the absence of a transfection reagent, and the sense oligonucleotide is a symmetric gapped oligomeric compound comprising a 5′ region consisting of three to five 2′-O—(CH a central region consisting of nine to twenty β-D-ribonucleosides; and a 3′ region consisting of three to five 2′-O—(CH thereby reducing the level of target mRNA in the cell.