Patent ID: 9193782
Filing Date: 2015-11-24
Classification: A61K,A61P,C07K,G01N

Abstract:
1. A method of treating a human patient with a breast tumor exhibiting a SPARC microenvironment signature comprising: (a) preparing a plurality of histological sections of the tumor to obtain a SPARC microenvironment signature by immunostaining a histological sections of the tumor with a first anti-SPARC antibody, wherein the first anti-SPARC antibody binds to the immunodominant epitopes bound by the anti-SPARC polyclonal antisera AF941, and immunostaining histological sections of the tumor with a second anti-SPARC antibody, wherein the second anti-SPARC antibody binds to the epitope bound by the anti-SPARC monoclonal antibody Mab941; (b) selecting a patient with a breast tumor having a SPARC microenvironment signature of least 70% of the tumor cells, fibroblasts, blood vessels, stroma and inflammatory cells staining positive with the first antibody, and at least 50% of the inflammatory cells and at least 70% of the blood vessels and stroma staining positive with the second antibody; and (c) administering a therapeutically effective amount of a chemotherapeutic regimen to the selected patient.