Patent ID: 6133505
Filing Date: 2000-10-17
Classification: C07K,C12N

Abstract:
A method for producing a transgenic plant with increased resistance or tolerance to a geminivirus comprising the steps of:deriving a mutated nucleotide sequence coding for an inactive protein C1 corresponding to the active protein C1 of a geminivirus, wherein the lysine located between positions 220 and 235 of said active protein C1 is replaced by alanine, arginine or histidine;introducing said mutated nucleotide sequence into a plant cell to produce a transformed plant cell; andregenerating a transgenic plant from the transformed plant cell, wherein said mutated nucleotide sequence induces a dominant negative phenotype of inhibited replication, movement or plant-to-plant distribution of a geminivirus, and wherein the transgenic plant has increased resistance or tolerance to a geminivirus compared to an untransformed plant.