Patent ID: 6773879
Filing Date: 2004-08-10
Classification: C12N,Y02A,Y02P

Abstract:
A method for identifying DNA for polymorphic forest tree plants, comprising the steps of:a) selecting two sibling individuals of a forest tree plant having different phenotypes; b) obtaining genomic DNA from the two individuals; c) selecting DNA fragments by an inter-individual genome subtraction method using the genomic DNA from the two individuals; d) providing a labeled cDNA probe derived from all mRNA obtained from the two individuals, wherein the cDNA is selected and amplified by oligonucleotide primers in a polymerase chain reaction; e) fractionating the DNA fragments obtained by the genome subtraction of step c) and screening the DNA fragments with the RNA-derived labeled probe of step d); f) performing intra-individual subtraction with genomic DNA from one of the two individuals; and g) comparing the DNA fragments of steps e) and f) to exclude the DNA fragments containing intra-individual polymorphisms and identifying the DNA fragments that are polymorphic between the individuals.