Patent ID: 9115390
Filing Date: 2015-08-25
Classification: C12N,C12Q

Abstract:
1. A method for identifying frameshift mutations in coding target nucleic acids comprising one or more stop codons which comprises the steps: (i) providing a host cell comprising a double-stranded nucleic acid, which comprises a coding target nucleic acid and a coding opposite strand nucleic acid complementary thereto, in which the opposite strand nucleic acid is linked via a linker with a reporter gene in 3′-position; wherein the linker comprises a translational coupler sequence which comprises a stop codon in frame to the reading frame of the opposite strand nucleic acid and a start codon, (ii) effecting expression of the opposite strand nucleic acid; and (iii) identifying whether expression of the reporter gene occurs in the host cell, in which expression of the reporter gene indicates that the target nucleic acid does not comprise a frameshift mutation.