Patent ID: 7354707
Filing Date: 2008-04-08
Classification: C12Q,Y02A

Abstract:
1. A method of determining the initial amounts of individual species of a target gene, comprising: amplifying a target gene and monitoring the amplification by real-time PCR; performing a polymorphous analysis with respect to the amplified target gene to determine a polymorphous composition ratio of individual species of the target gene; and determining the initial amount of the target gene from the percentage of a change in the intensity of fluoresence occurring as a result of hybridization of the nucleic acid probe to the amplified target gene; and determining the initial amounts of individual species of the target gene by multiplying the initial amount of the target gene by said polymorphous composition ratio of individual species of the target gene, wherein the real-time PCR is accomplished with a nucleic acid probe, wherein the probe comprises a single-stranded oligonucleotide capable of hybridizing to the target gene, wherein the probe is labeled with a fluorescent dye and a quencher substance, wherein the oligonucleotide is labeled with the fluorescent dye and the quencher substance such that the intensity of fluorescence in a hybridization reaction system increases when the probe is hybridized with the target gene, and wherein the oligonucleotide forms no stem-loop structure between bases at positions where the oligonucleotide is labeled with the fluorescent dye and the quencher substance.