Patent ID: 6544506
Filing Date: 2003-04-08
Classification: A61K,C12N,Y02A

Abstract:
A method of preventing or decreasing cell mediated graft versus host disease (GVHD) and graft rejection of a transplant derived from an allogeneic donor in a recipient of the transplant, the method comprising the steps of:(a) transplanting the trasplant into the recipient; (b) administering to the recipient a dose including non-alloreactive ani-third party cytotoxic T-lymphocytes (CTLs) and being substantially depleted of T-lymphocytes capable of developing into anti-recipient alloreactive CTLs, wherein said non-alloreactive anti-third party CTLs are generated by directing T-lymphocytes of the donor against a third party antigen or antigens and wherein depletion of T-lymphocytes capable of developing into said anti-recipient alloreactive CTLs is effected by deprivation of a factor which is (i) required for CTL maturation; and (ii) secreted by maturing CTLs; and (c) conditioning the recipient under sublethal, lethal or supralethal conditions, thereby preventing or decreasing cell mediated GVHD and graft rejection of the transplant.