Patent ID: 8647880
Filing Date: 2014-02-11
Classification: C07K,G01N,Y10T

Abstract:
1. A method of detection of an amino acid sequence for identification of peptides and/or proteins, the method comprising the following steps: selective derivatization of an N-terminus of a peptide or an N-terminus of a protein having an unprotected ε-amine of lysine versus derivatization of the unprotected ε-amine of lysine, wherein the selective derivatization is carried out at pH 1-5 with addition of sodium cyanoborohydride (NaBH mass spectrometer analysis of the derivatized analytes by acquiring spectra of derivatized negative ions in a negative mode of operation of a mass spectrometer; determining an amino acid sequence of the derivatized analytes by interpretation of fragmentation sequence data obtained from the mass spectrometer analysis.