Patent ID: 8889849
Filing Date: 2014-11-18
Classification: C07K,C12N,G01N

Abstract:
1. An isolated genotype 4a hepatitis C viral (HCV) RNA construct comprising a 5′NTR, an internal ribosome entry site (IRES), sequence encoding one or more of NS3, NS4A, NS4B, NS5A or NS5B, and a 3′NTR, wherein the RNA construct further comprises a mutation as compared to a wild-type HCV 4a sequence of SEQ ID NO:8, wherein said mutations is T343K, T343R, A200E, or T511R in NS3, or Q34K, Q34R, or E52V in NS4A, or L179P in NS5A, and wherein the mutation positions correspond to the residue positions in SEQ ID NO:9.