Patent ID: 7229758
Filing Date: 2007-06-12
Classification: C07K,C12Q,G01N

Abstract:
1. A method of determining the presence or absence in a human patient specimen of a specific alteration in sequence of a km23 nucleic acid form relative to the wild-type km23 nucleic acid form encoding SEQ ID NO: 1, the method comprising: (a) providing RNA from a human patient specimen; (b) providing a first pair of PCR primers which span the entire open reading frame of nucleic acid encoding human km23 of SEQ ID NO: 1; (c) performing a first round of nested RT-PCR using (i) said RNA as the reaction template and (ii) said first primer pair under suitable conditions to obtain a first PCR product; (d) providing a second pair of PCR primers capable of binding within said first PCR product; (e) performing a second round of nested RT-PCR using said first PCR product as the reaction template under suitable conditions to obtain a second PCR product; and (f) sequencing and aligning said second PCR product with a wild-type km23 nucleic acid form encoding the km23 polypeptide of SEQ ID NO: 1 to determine a specific alteration in sequence wherein the specific alteration indicates an increased risk with respect to ovarian cancer.