Patent ID: 8883438
Filing Date: 2014-11-11
Classification: A61K,C12N,C12Q

Abstract:
1. A method of diagnosing a subject having a cell proliferation-related disorder or suspected of having a cell proliferation-related disorder characterized by: (a) the presence, distribution, or level of an isocitrate dehydrogenase 1 enzyme having a mutation at residue 97 wherein the glycine residue has been replaced with an aspartic acid residue (IDH1-G97D), which has 2-hydroxyglutarate (2HG) neoactivity, wherein 2HG neoactivity is the ability to convert alpha ketoglutarate to 2-hydroxyglutarate, or (b) elevated levels of 2HG due to the presence of IDH1-G97D mutant enzyme having 2HG neoactivity, wherein said method comprises analyzing the presence, distribution, or level of 2HG in a tissue, product, or bodily fluid of said subject by a chromatographic method, wherein an increased presence, distribution, or level of 2HG indicates the presence of the cell proliferation-related disorder, thereby diagnosing the subject for the cell proliferation-related disorder.