Patent ID: 6329208
Filing Date: 2001-12-11
Classification: G01N,G01R,Y10T

Abstract:
A method for determining relative pyruvate cycling flux in a mammal, comprising:a) administering (1,2,3-.sup.13 C.sub.3) propionate, (1,2,3-.sup.13 C.sub.3) lactate, (1,2,3-.sup.13 C.sub.3) pyruvate, or (1,2,3-.sup.13 C.sub.3) alanine to said mammal;b) obtaining a blood sample from said mammal; andc) determining a relative rate of pyruvate cycling from a .sup.13 C NMR spectrum of .sup.13 C-labeled glutamate or gluconate in said sample,wherein the relative pyruvate cycling flux is calculated from the ratio of area under selected [.sup.13 ]C2 peaks of glutamate or gluconate.