Patent ID: 6743598
Filing Date: 2004-06-01
Classification: A61K,C12Q,G01N

Abstract:
A method for the detection of phosphoglucose isomerase, ketol-isomerase and acetyltransferase activities in a sample, comprising the steps of:a) providing: i) a sample suspected to contain phosphoglucose isomerase, ketol-isomerase and acetyltransferase activities, ii) glucose-6-phosphate, iii) glutamine, iv) acetyl coenzyme A, and v) 5,5â€²-dithiobis(2-nitrobenzoic acid); b) combining said sample, said glucose-6-phosphate, said glutamine, and said acetyl coenzyme A under conditions to yield reaction products comprising coenzyme A and N-acetylglucosamine-6-phosphate; c) inactivating said phosphoglucose isomerase, ketol-isomerase and acetyltransferase activities; and d) combining said reaction product comprising coenzyme A and 5,5â€²-dithiobis(2-nitrobenzoic acid) under conditions to yield a chromogenic reaction product comprising 2-nitro-thiobenzoate anion, wherein said chromogenic reaction product is indicative of phosphoglucose isomerase, ketol-isomerase and acetyltransferase activities.