Patent ID: 6812376
Filing Date: 2004-11-02
Classification: A61K,C07K,G01N

Abstract:
A method for identifying a compound that modulates a biological activity of a serotonin-gated anion channel, said method comprising the steps of:(a) contacting a cell with a first purified nucleic acid sequence that hybridizes, under conditions comprising hybridization at about 42Â° C. in about 50% formamide followed by a first wash at about 65Â° C. in about 2Ã—SSC sodium chloride/sodium citrate solution and about 1% Sodium Dodecyl Sulfate, and a second wash at about 65Â° C. in about 1Ã—SSC sodium chloride/sodium citrate solution and about 0.1% Sodium Dodecyl Sulfate, to a second nucleic acid sequence comprising the complement of the sequence of SEQ ID NO:2, wherein said first nucleic acid sequence encodes a serotonin-gated anion channel that selectively permits passage of anions into or out of said cell in response to binding serotonin, and wherein said first nucleic acid sequence is expressed in said cell; (b) administering a test compound to said cell; and (c) assaying a modulation in current flux into or out of said cell, wherein a modulation in current flux into or out of said cell, relative to a control cell not contacted with said first nucleic acid sequence, is indicative of a compound that modulates said biological activity of said serotonin-gated anion channel.