Patent ID: 7153433
Filing Date: 2006-12-26
Classification: C12N

Abstract:
1. A method for purifying nucleic acids present in a raw material, the method comprising: providing a flow-permeable membrane or matrix having a surface functionalized with deprotonable groups that are bound to polymer chains, the polymer chains having a first end that is fixed to the membrane or matrix and a second end that is displaceable away from the membrane or matrix; permeating the membrane or matrix with a solution comprising the raw material and a binding buffer, wherein the pH value of the solution is less than or about the same as the pK value of the surface of the membrane or matrix such that the polymer chains rest against the membrane or matrix to cause the nucleic acids in the solution to become selectively bound to the surface of the membrane or matrix; and eluting the nucleic acids bound to the surface of the membrane or matrix by permeating the membrane or matrix with an elution buffer having a pH value that is greater than the pK value of the surface of the membrane such that the second ends of the polymer chains are displaced away from the membrane or matrix.