Patent ID: 6130073
Filing Date: 2000-10-10
Classification: C12Q,Y10S

Abstract:
A method for detecting a target polynucleotide in a sample comprising:amplifying the target polynucleotide by primer extension to form an amplification product that is complementary to the target polynucleotide, the amplification being performed (a) in the presence of at least one pair of oligonucleotide probes capable of hybridizing to contiguous sequences of the amplification product, and (b) at a higher temperature than a melting point temperature of the oligonucleotide probes;performing multiple ligation cycles during which oligonucleotide probes which hybridize to contiguous sequences of the amplification product are ligated to form an oligonucleotide probe ligation product; anddetecting the oligonucleotide probe ligation product.