Patent ID: 9120850
Filing Date: 2015-09-01
Classification: C07K,G01N

Abstract:
1. A labeled protein comprising one of the following sequences: amino acids 2-178 of SEQ ID NO: 1 wherein at least one amino acid of positions 27, 89 or 93 is substituted with a fluorescently labeled cysteine; amino acids 2-162 of SEQ ID NO: 7 wherein at least one amino acid of positions 27 and 89 is substituted with a fluorescently labeled cysteine; amino acids 2-175 of SEQ ID NO: 8 wherein at least one amino acid of positions 27 and 89 is substituted with a fluorescently labeled cysteine; amino acids 2-170 of SEQ ID NO: 9 wherein at least one amino acid of positions 23 and 82 is substituted with a fluorescently labeled cysteine; amino acids 2-152 of SEQ ID NO: 10 wherein at least one amino acid of positions 22 and 83 is substituted with a fluorescently labeled cysteine; amino acids 2-162 of SEQ ID NO: 11 wherein at least one amino acid of positions 26 and 88 is substituted with a fluorescently labeled cysteine; and amino acids 2-179 of SEQ ID NO: 12 wherein at least one amino acid of positions 23 and 82 is substituted with a fluorescently labeled cysteine; and wherein the fluorescent label emits a signal that is greater when the labeled protein is bound to single-stranded DNA as compared to when the labeled protein is not bound to single-stranded DNA.