Patent ID: 7897377
Filing Date: 2011-03-01
Classification: C12M

Abstract:
1. A device for organotypic culture, said device comprising: first and second medium conduits, each having one open end and one end closed by a porous membrane fused across it; and a frame holding at least the first medium conduit in a substantially vertical orientation wherein each of the first and second medium conduits is adapted to permit retention by capillarity of a sufficient volume of liquid culture medium in the medium conduit to contact the surface of the porous membrane and thus supply nutrients to cells that may be grown on the porous membrane when the device is in either the upright or the inverted position, wherein the device further comprises a cell suspension conduit having one end open and one end closed by the surface of said porous membrane contralateral to the surface of said porous membrane sealed at least to said first medium conduit; wherein said frame holds at least the first medium conduit and the cell suspension conduit in a substantially vertical orientation wherein the device further comprises a chamber enclosing the open end of at least the first medium conduit; wherein the chamber comprises an opening to allow culture medium to be supplied independently to the first and second medium conduits.