Patent ID: 7262038
Filing Date: 2007-08-28
Classification: C12N,C12Q,G01N

Abstract:
1. A polynucleotide containing at least one copy of an expression cassette comprising, in the reading frame direction of transcription, polynucleotide sequences comprising: a promoter region of a methanol responsive gene of a methylotrophic yeast; a polynucleotide sequence encoding simplified nitrate reductase (S-NaR1) polypeptide; a transcription terminator functional in methylotrophic yeast, wherein said polynucleotide sequences are operationally associated so that when transcribed and translated in a host, the S-NaR1 polypeptide is produced intracellularly and, after incorporation of the molybdenum-molybdopterin cofactor from the host in vivo, is catalytically active for reduction of nitrate to nitrite; and wherein the S-NaR1 polypeptide has a sequence according to SEQ ID NO: 1, optionally truncated at amino acid 483.