Patent ID: 6451561
Filing Date: 2002-09-17
Classification: A61P,C07K,C12N,C40B,G01N,Y10S

Abstract:
A method for forming a human growth hormone variant, said method comprising substituting at least one different amino acid for at least one of the active amino acid residues in human growth hormone, said active amino acid residue selected from the group consisting of P2, T3, P5, S7, L9, N12, L15, R16, R19, E30, E33, S43, F44, Q46, N47, P48, Q49, T50, F54, S55, E56, S57, I58, P59, S62, N63, E66, Q68, Q69, K70, S71, L73, R77, L80, F97, A98, N99, S100, L101, V102, Y103, G104, D169, T175, R178, Q181, C182, R183, S184, V185, E186, G187, S188, and F191, numbered from the N-terminus of 191-amino acid human growth hormone, to form a human growth hormone variant wherein the amino acid sequence of said variant is not found in nature and said variant binds to a target for said human growth hormone with an affinity different from the affinity of said human growth hormone for said target.