Patent ID: 6117638
Filing Date: 2000-09-12
Classification: C12N

Abstract:
A method of identifying test compounds that modulate transcription of a gene of interest by modulating the triggering function of a transcription factor, the method comprising:i) contacting a nucleic acid with a tethered coactivator, said nucleic acid comprising a reporter gene operably linked to a response element, and said tethered coactivator comprising:a) a polypeptide that comprises an activation function from a transcriptional coactivator; andb) a DNA binding moiety that specifically binds to the response element;ii) contacting the tethered coactivator with a transcription factor polypeptide that comprises an activation function of a transcription factor that is involved in modulating expression of the gene of interest in its natural milieu, wherein the transcription factor polypeptide is contacted with a test compound prior to contacting the tethered coactivator; andiii) detecting expression of the reporter gene wherein an alteration of the expression of the reporter gene as compared to expression of the reporter gene where the transcription factor polypeptide is not contacted with the test compound indicates that said test compound modulates the triggering function of the transcription factor.