Patent ID: 6027922
Filing Date: 2000-02-22
Classification: A61K,C07K,C12N,Y10S

Abstract:
A method for producing a monoclonal antibody showing an increased binding to THP-1 foam cells compared to its binding to THP-1 cells treated for 24 hours with a mixture containing 2.times.10.sup.-7 M PMA and 0.5.times.10.sup.-7 M retinoic acid:said monoclonal antibody recognizing an antigen binding to the monoclonal antibody 3.A9F5E1 produced by a hybridoma cell line deposited in the ECACC under No. 93102638 on Oct. 26, 1993; said THP-1 foam cells having been deposited at the ATCC under No. TIB-202 on Oct. 1, 1983, said method comprising the steps of:(a) immunizing an animal with KG-1a cells,(b) fusing the spleen cells of said animal with myeloma cells to form antibody secreting hybridoma cells,(c) screening the hybridoma cells for production of monoclonal antibodies directed against KG-1a cells, and(d) screening the hybridoma cells scoring positive in step (c) for production of a monoclonal antibody which shown an increased binding to THP-1 foam cells compared to its binding to THP-1 cells treated for 24 hours with a mixture containing 2.times.10.sup.-7 M PMA and 0.5.times.10.sup.-7 retinoic acid,(e) culturing the selected hybridoma according to step (d) in a culture medium,(f) recovering the monoclonal antibodies secreted by the selected hybridomas cultured as in (e), or alternatively,(g) implanting the selected hybridomas as in (e) into the peritoneum of a mouse and, when ascites has been produced by the animal, recovering the monoclonal antibodies, said KG-1a cells having been deposited on Dec. 12, 1984, at ATCC CCL 246.1 with the ATCC.