Patent ID: 8962331
Filing Date: 2015-02-24
Classification: C12N

Abstract:
1. A method of producing human iPS cells, comprising: contacting a population of human adipose stem cells with one or more minicircle DNA vectors encoding a plurality of reprogramming factors operably linked to a promoter; wherein the plurality of reprogramming factors comprises Oct4, Sox2, Lin28, and Nanog, or Oct4, Sox2, c-Myc, and Klf4; and maintaining the cells for a period of time sufficient to reprogram said human adipose stem cells to pluripotency in the absence of genomic integration of sequences of the one or more minicircle DNA vectors, wherein each of the one or more minicircle DNA vectors is 0.3-10 Kb in length, lacks an origin of replication, and lacks a drug selectable marker.