Patent ID: 8691729
Filing Date: 2014-04-08
Classification: C12N

Abstract:
1. A method of identifying new chemical reactions, the method comprising steps of: providing one or more single-stranded DNA templates, wherein each template has a unique pair of substrates covalently linked to the template; wherein each substrate comprises one or more functional groups selected from the group consisting of aliphatic halides, aryl halides, esters, amides, carbonates, carbamates, ureas, alcohols, thiols, amines, alkyls, alkenes, alkynes, aryls, heteroaryls and phosphorus-containing groups; wherein a portion of the sequence of the single-stranded DNA template encodes the identity of the two substrates covalently linked to the template; and wherein one of the two substrates and biotin is covalently linked to the template through a cleavable linker; subjecting the single-stranded DNA templates with the substrates covalently linked to the template to reaction conditions, whereby a covalent bond is formed directly between each of the two different substrates covalently linked to a single template; cleaving the cleavable linker; selecting unique substrate pairs that reacted to form the a covalent bond between the two substrates that form the unique pair of substrates covalently linked to the template using streptavidin; and identifying the selected unique substrate pairs that reacted using the encoded single-stranded DNA template covalently liked to the unique substrate pairs and reaction conditions.