Patent ID: 6623926
Filing Date: 2003-09-23
Classification: A61K,C07K

Abstract:
A method for generating a 5â€²-nucleic acid-protein conjugate, said method comprising contacting a non-derivatized protein and a nucleic acid which carries a reactive group at its 5â€² end under conditions that allow said reactive group to react with an N-terminus of said non-derivatized protein, thereby forming a 5â€²-nucleic acid-protein conjugate, wherein said non-derivatized protein comprises an N-terminal cysteine and the nucleic acid reactive group is an aminothiol reacting group, or wherein said non-derivatized protein comprises an &agr;-helical tetracysteine motif located proximal to the N-terminus and the nucleic acid reactive group is a bisarsenical group that is reactive with the tetracysteine group.