Patent ID: 8809009
Filing Date: 2014-08-19
Classification: C12Q,G01N

Abstract:
1. A method of determining the presence, identity, and/or severity of a disease or condition in an individual wherein the disease or condition is associated with abnormal glycan biosynthesis, degradation, or accumulation, the method comprising: (a) generating a first biomarker comprising a glycan residual compound, wherein the first biomarker is generated by treating a population of glycans, in or isolated from a biological sample from the individual, with at least one digesting glycan enzyme, (b) generating a second biomarker comprising a glycan residual compound, wherein the second biomarker is generated by treating a population of glycans, in or isolated from a biological sample from the individual, with at least one digesting glycan enzyme in the same or different digestion step as provided in step (a), (c) using an analytical instrument to detect the presence of and/or measure the amount of the first and second biomarker produced and displaying or recording the presence of or a measure of a population of the first and second biomarkers, and (d) monitoring and/or comparing the amounts of the first and second biomarkers in a biological sample;