Patent ID: 6710164
Filing Date: 2004-03-23
Classification: C07K,C12Q

Abstract:
A compound having the formula: wherein:R8 is H or an amine protecting group; R9 is H, alkyl having from 1 to about 12 carbon atoms, or alkenyl having from 2 to about 12 carbon atoms; one of R4 and R5 is H and the other of R4 and R5 is a moiety of formula: whereinR10 is an amine protecting group; Lâ€² is selected from the group consisting of hydrogen, hydroxy, (C1-C4)alkanoyl, naturally occurring nucleobases, non-naturally occurring nucleobases, aromatic moieties, DNA intercalators, nucleobase-binding groups, and heterocyclic moieties, reporter ligands, wherein amino groups are, optionally, protected by amino protecting groups; A is a group of formula (IIa)-(IId): where:X is O, S, Se, NR3, CH2 or C(CH3)2; Y is a single bond, O, S or NR4 where R4 is as described above; each r and s is zero or an integer from 1 to 5; each R1 and R2 is independently selected from the group consisting of hydrogen, (C1-C4)alkyl which may be hydroxy- or alkoxy- or alkylthio-substituted, hydroxy, alkoxy, alkylthio, amino and halogen; and R3 is selected from the group consisting of hydrogen, (C1-C4)alkyl, hydroxy- or alkoxy- or alkylthio-substituted (C1-C4)alkyl, hydroxy, alkoxy, alkylthio and amino.