Patent ID: 9101930
Filing Date: 2015-08-11
Classification: B01L,B29C,B29L,C12M,C12N,C12Q

Abstract:
1. A cartridge, configured to facilitate processing and detecting nucleic acid in a sample, comprising: a first layer comprising a sample port and a fluid port; an intermediate substrate coupled to the first layer and defining a waste chamber with a corrugated surface directly opposing the first layer, wherein the corrugated surface defines a set of voids external to the waste chamber and accessible from a direction perpendicular to a broad surface of the first layer; and a fluidic pathway, superior to the intermediate substrate, at least partially separated from the corrugated surface of the waste chamber by an elastomeric laver, and formed by at least a portion of the first layer, wherein the fluidic pathway is fluidically coupled to the sample port and the fluid port, wherein the fluidic pathway is configured to be occluded at a set of occlusion positions through at least one void of the set of voids of the corrugated surface by deformation of the elastomeric layer, and wherein the fluidic pathway is configured to transfer waste fluid to an interior portion of the corrugated surface of the waste chamber, through a set of openings of the intermediate substrate, upon occlusion of a subset of the set of occlusion positions.