Patent ID: 6159719
Filing Date: 2000-12-12
Classification: C07K,C12N,C12Q,C12Y,G01N

Abstract:
A murein binding polypeptide in vitro diagnostic reagent for detecting a eubacteria or a fungus in a biological sample, whereinsaid murein binding polypeptide in vitro diagnostic reagent comprises a murein binding polypeptide chemically conjugated to a signal generating compound;said murein binding polypeptide comprises a catalytically inactive enzyme polypeptide having a binding site capable of binding to either a eubacterial murein compound or a fungal murein like compound with a binding affinity of about 5.times.10.sup.-7 L/mol to about 5.times.10.sup.-9 L/mol;and wherein said conjugate comprising said murein binding polypeptide and signal generating compound is effective when bound to said eubacteria or said fungus to produce a detectable signal in a diagnostic assay format.