Patent ID: 6376190
Filing Date: 2002-04-23
Classification: C12N,C12Q

Abstract:
A method for generating nucleic acid ligands to a target protein, comprising:a) providing a peptide, said peptide comprising a linear amino acid sequence identical to at least a portion of said target protein; b) providing a candidate mixture of nucleic acids; c) contacting the candidate mixture of nucleic acids with said peptide, wherein nucleic acids having an increased affinity to said peptide relative to the candidate mixture may be partitioned from the remainder of the candidate mixture; d) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture; e) amplifying the increased affinity nucleic acids to yield a candidate mixture of nucleic acids enriched for nucleic acids with relatively higher affinity and specificity for binding to said peptide; f) contacting said enriched candidate mixture with a complex mixture containing said target protein, wherein nucleic acids having an increased affinity to said target protein relative to the enriched candidate mixture may be partitioned from the remainder of the candidate mixture; g) partitioning the increased affinity nucleic acid ligands in f) from the remainder of the enriched candidate mixture; h) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acids with relatively higher affinity and specificity for binding to said target protein; whereby nucleic acid ligands to said target protein may be identified.