Patent ID: 6670144
Filing Date: 2003-12-30
Classification: C12Q,G01N

Abstract:
A method of screening for a candidate modulator of enzymatic activity of a kinase or a phosphatase, the method comprisinga) contacting an isolated natural binding domain, a binding partner therefor and an enzyme with a candidate modulator of said kinase or phosphatase, wherein said natural binding domain includes a site for post-translational phosphorylation and binds said binding partner in a manner that is dependent upon phosphorylation or dephosphorylation of said site by said kinase or phosphatase and wherein at least one of said isolated natural binding domain and said binding partner comprises a detectable label; b) detecting the binding or dissociation of said isolated natural binding domain to/from said binding partner; and c) monitoring the binding of said isolated natural binding domain to said binding partner, wherein binding or dissociation.of said isolated natural binding domain and said binding partner as a result of said contacting is indicative of modulation of enzymatic activity by said candidate modulator of said kinase or phosphatase.