Patent ID: 7344714
Filing Date: 2008-03-18
Classification: C07K

Abstract:
1. A method for producing an antibody that specifically binds a single modified amino acid selected from the group consisting of a glycosylated amino acid, an acetylated amino acid, and a methylated amino acid, said method comprising the steps of: (a) constructing a degenerate peptide library comprising (i) a single fixed modified amino acid selected from the group consisting of a glycosylated amino acid, an acetylated amino acid, and a methylated amino acid, and (ii) a plurality of degenerate amino acids flanking said modified amino acid; (b) immunizing a host with said peptide library to raise a context-independent antibody specific for said modified amino acid; and (c) isolating antisera from said host, and purifying the context-independent antibody of step (b) from said antisera, said antibody specifically binding said single modified amino acid independent of the peptide context in which it occurs.