Patent ID: 7858317
Filing Date: 2010-12-28
Classification: C12Q

Abstract:
1. A method of diagnosing breast cancer or ductal carcinomas in situ (DCIS) in a subject comprising determining the state of methylation of one or more CpG islands in the promoter of Twist nucleic acids isolated from a sample comprising blood, plasma, lymph, duct cells, ductal lavage fluid, nipple aspiration fluid, breast tissue, lymph nodes, bone marrow, or a combination thereof of the subject, wherein a state of hypermethylation of one or more CpG islands in the promoter of Twist nucleic acids as compared with the state of methylation of one or more CpG islands in the promoter of Twist nucleic acids in comparable samples obtained from normal subjects is indicative of breast cancer or DCIS in the subject.