Patent ID: 6333396
Filing Date: 2001-12-25
Classification: C07K,C12N

Abstract:
A genetically engineered single-chain antigen-binding polypeptide comprising:(a) a first polypeptide comprising the antigen binding portion of the variable region of an antibody heavy or light chain;(b) a second polypeptide comprising the antigen binding portion of the variable region of an antibody heavy or light chain; and(c) a peptide linker linking the first and second polypeptides (a) and (b) into a single chain polypeptide having an antigen binding site,wherein at its C-terminus, N-terminus, or both, the single-chain antigen-binding polypeptide comprises basic amino acid residues selected from the group consisting of: oligo-Lys, oligo-Arg and a combination thereof.