Patent ID: 6569626
Filing Date: 2003-05-27
Classification: C12Q

Abstract:
A method of simultaneous detection of at least two preselected target chromosomes or target chromosome regions by in situ hybridization using multiple direct label DNA probes comprising:(a) providing a plurality of at least two direct label DNA probes compositions, wherein each of the direct label DNA probe compositions: (i) comprise direct label DNA segments which are complementary to a single target chromosome or target chromosome region and have fluorescent labels directly linked to the DNA segments, and (ii) is detectable by use of a different fluorescent color, each color being detectable in the presence of other direct label probe compositions; (b) contacting the plurality of direct label DNA probe compositions under hybridizing conditions in in situ hybridization with a plurality of target chromosomes or target chromosome regions contained in a target specimen; and (c) detecting the target chromosomes or target chromosome regions by identification of two or more fluorescent colors using a digital image of the target specimen.