Patent ID: 8945872
Filing Date: 2015-02-03
Classification: C07K

Abstract:
1. A method of purifying recombinant GDF-5 protein from a cell, the method comprising: providing a comprising an expression vector having a T5 or a Trc promoter operatively linked to a polynucleotide sequence that encodes a GDF-5 protein, and recovering the GDF-5 protein from the cell by contacting the GDF-5 protein with a substrate so as to isolate the GDF-5 protein, wherein the vector is a plasmid vector that is pGDF5-Trc or pGDF5-T5, the T5 or Trc promoter is inducible by isopropylthio-β-galactoside (IPTG), the pGDF5-T5 comprises the polynucleotide sequence of SEQ ID NO: 2 and the pGDF5-Trc comprises the polynucleotide sequence of SEQ ID NO: 3.