Patent ID: 6013444
Filing Date: 2000-01-11
Classification: C12Q

Abstract:
Primer sets which produce locus compatible markers for determining DNA fragment lengths of a polymorphic region, comprising:primary forward and primary reverse primers which respectively hybridize to upstream and downstream sequences which flank a polymorphic region of a DNA sample, at least one of said primary forward and primary reverse primers including a first detectable label, said primary forward primer hybridizing to an antisense strand of DNA of said DNA sample, said primary reverse primer hybridizing to a sense strand of DNA of said DNA sample, said primary forward and primary reverse primers encompassing a first section in said DNA sample which includes said polymorphic region; andfirst and second secondary reverse primers which hybridize to said sense strand of DNA at first and second locations, respectively, each of which are downstream of said polymorphic region, said first location located within said first section of said DNA sample, the 3' end of said second location located downstream of said first section.