Patent ID: 6277573
Filing Date: 2001-08-21
Classification: C12Q,G01N,H01J,Y10T

Abstract:
A process for detecting the presence of a target nucleic acid sequence in a biological sample, comprising the steps of:a) performing on a nucleic acid molecule comprising a target nucleic acid sequence, a first polymerase chain reaction comprising a first forward primer and a first reverse primer, which can amplify a portion of the nucleic acid molecule comprising at least a portion of the target nucleic acid sequence, thereby producing a first amplification product;b) performing on the first amplification product, a second polymerase chain reaction comprising a second forward primer and a second reverse primer, which can amplify at least a portion of the first amplification product comprising at least a portion of the target nucleic acid sequence, thereby producing a second amplification product; andc) detecting the amplification product by mass spectrometry, thereby detecting the presence of the target nucleic acid sequence in the biological sample.