Patent ID: 8445221
Filing Date: 2013-05-21
Classification: C12N,C12Q

Abstract:
1. An isolated modified glucose dehydrogenase (GLD), comprising an amino acid sequence of a wild-type FAD-conjugated glucose dehydrogenase (GLD) represented by SEQ ID NO: 1 having a substitution of at least one amino acid residue selected from the group consisting of D72A, G73D, G73A, G73S, G73C, G73Q, G73W, G73Y, G73E, G73H, R102H, Y228H, V356A, P527L, S37V, S37G, T69I, L76F, F78L, R102V, N217S, P240I, P240L, Q407A, Q407S, Y424S, A437I, and T530A, wherein a value of a ratio of [activity for xylose]/[activity for glucose] for the isolated modified GLD is 0.85 times or less than a value of a ratio of [activity for xylose]/[activity for glucose] of the wild-type FAD-conjugated GLD.