Patent ID: 9080217
Filing Date: 2015-07-14
Classification: C12Q

Abstract:
1. A method of detecting C. difficile in a sample, the method comprising: performing an amplifying step comprising contacting the sample with a set of tcdB primers to produce an amplification product if a tcdB nucleic acid is present in the sample; performing a hybridizing step comprising contacting the sample and the amplification product, if produced, with a detectable tcdB probe; and detecting the presence or absence of the amplification product, wherein the presence of the amplification product is indicative of the presence of wherein the set of tcdB primers comprises a first primer consisting of the sequence of SEQ ID NO: 2 or the complement thereof, and a second primer consisting of the sequence of SEQ ID NO: 4 or the complement thereof; wherein the detectable tcdB probe comprises an oligonucleotide consisting of the sequence of SEQ ID NO: 9 or the complement thereof, and wherein the detectable tcdB probe is labeled with a donor fluorescent moiety and a corresponding acceptor moiety; and wherein the detecting comprises detecting the presence or absence of fluorescence resonance energy transfer (FRET) between the donor fluorescent moiety and the acceptor moiety of the probe, wherein the presence or absence of fluorescence is indicative of the presence or absence of