Patent ID: 6391568
Filing Date: 2002-05-21
Classification: G01N,Y10S

Abstract:
A method for measuring platelet reactivity in a whole blood sample without preparing a platelet-rich preparation, consisting essentially of:(A) activating a portion of the platelets in the whole blood sample by adding to the sample an amount of a platelet agonist, thereby providing the whole blood sample containing activated platelets and unactivated platelets; (B) exposing the whole blood sample from (A) to the following agents: an activation-dependent fluorochrome-labeled ligand comprising a ligand that binds one form more activated platelets; an activation-independent fluorochrome-labeled ligand comprising a ligand that binds both said activated platelets and said unactivated platelets; a binding ligand which binds all platelets to a solid surface; a fixative which prevents further platelet activation; (C) exposing the whole blood sample from (B) to a solid surface having a moiety that binds said binding ligand which binds all platelets to a solid surface, to allow binding of all platelets to the solid surface; (D) measuring fluoroscence of the activation-dependent fluorochrome-labeled ligand and the activation-independent fluorochrome-labeled ligand; and (E) determining a ratio of fluorescence of the activation-dependent fluorochrome-labeled ligand to the activation-independent fluorochrome-labeled ligand, thereby measuring platelet reactivity.