Patent ID: 8846357
Filing Date: 2014-09-30
Classification: A61L,C08H,C08L,C12H,C12N,C12P,C12Y,Y02E

Abstract:
1. A fermentation method for controlling bacterial contamination in a fermentation process for making ethanol comprising a Zymomonas biocatalyst comprising: a) providing a first fermentation medium lacking a cellulosic biomass hydrolysate selected from the group consisting of: i) a defined medium, ii) a medium containing a non-cellulosic biomaterial sugar source, and iii) a clarified cellulosic biomass hydrolysate; b) inoculating the first fermentation medium of a) with c) growing the d) providing a second fermentation medium or a saccharification reaction slurry; e) inoculating the second fermentation medium or the saccharification reaction slurry of d) with the seed culture of c) to form a fermentation broth; and f) growing the wherein; the second fermentation medium contains cellulosic biomass hydrolysate; and wherein: stabilized chlorine dioxide is added to at least one of i) the first fermentation medium of step a), ii) the saccharification reaction slurry of step d), or iii) the second fermentation medium of step d), and wherein: if the stabilized chlorine dioxide is added to the first fermentation medium of step a) the first fermentation medium is maintained at a temperature of at least 33° C. for at least 8 hours prior to inoculation with the wherein: if the stabilized chlorine dioxide is added to the saccharification reaction slurry of step d) the saccharification reaction slurry is maintained at a temperature of at least 33° C. for at least 6 hours prior to inoculation with the wherein: if the stabilized chlorine dioxide is added to the second fermentation medium of step d) the fermentation medium is maintained at a temperature of at least 33° C. for at least 6 hours prior to inoculation with the wherein: any one of the seed culture, the inoculated second fermentation medium or the fermentation broth comprising