Patent ID: 8415463
Filing Date: 2013-04-09
Classification: C12N

Abstract:
1. Nucleic acid comprising or encoding an RNA replicon comprising, in this order, the following segments (i) to (iii): (i) a nucleic acid sequence encoding a potexvirus RNA-dependent RNA polymerase; (ii) a nucleic acid sequence comprising: (iii) a heterologous nucleic acid sequence comprising a coding sequence of a protein of interest and a sub-genomic promoter for expressing said protein of interest from said replicon in a plant or in plant tissue; wherein said potexvirus triple gene block encodes three proteins as follows: a first protein comprising the protein sequence of SEQ ID NO: 10; a second protein comprising the protein sequence of SEQ ID NO: 12; and a third protein comprising the protein sequence of SEQ ID NO: 14, wherein said potexviral coat protein comprises a protein sequence of SEQ ID NO: 8, and wherein said sequence of item (i) encodes a protein having a sequence identical to a protein encoded by SEQ ID NO:4.