Patent ID: 6094274
Filing Date: 2000-07-25
Classification: G01N,G02B

Abstract:
A fluorescence detecting device comprising a light source, an illumination optical system for irradiating the light emitted from said light source to a specimen, an excitation filter set disposed in said illumination optical system for allowing a light with a specified wavelength region to transmit, a detecting optical system for detecting a fluorescence emitted from said specimen, and an absorption filter set disposed in the detecting optical system for allowing the fluorescence to transmit, whereinsaid excitation filter set includes at least two interference films with a transmittance of 25% or more at a wavelength by 20 nm shorter than the cross-over wavelength, and satisfies the following condition:0.05%<T<10%where T denotes the transmittance of said interference film at said cross-over wavelength, and the cross-over wavelength is defined as a wavelength at the crossing point between the transmittance spectrum at a longer wavelength side of said excitation filter set and the transmittance spectrum at a shorter wavelength side of said absorption filter set.