Patent ID: 9196465
Filing Date: 2015-11-24
Classification: G01N,H01J

Abstract:
1. A method for detecting hepcidin, comprising the following steps: controlling a sample liquid to contact with a nano chip, and then removing the sample liquid to obtain a nano chip that has been in contact with the sample, wherein, the sample liquid contains or doesn't contain hepcidin; eluting the nano chip that has been in contact with the sample liquid with an eluent, to obtain an eluate, wherein, the eluent can dissolve hepcidin; obtaining a mass spectrometric detection result of the eluate; determining whether the eluate contains hepcidin or determining the content of hepcidin in the eluate, according to the mass spectrometric detection result; wherein the nano chip comprises a substrate and a porous silicon oxide coating attached to the substrate, wherein the average pore size refers to the value obtained by measurement with an N wherein the contact angle of the surface of the porous silicon oxide coating is smaller than 15°, wherein the contact angle used is the value obtained by observing the contour of a water drop on solid surface, drawing tangent lines at the point of intersection among solid phase, liquid phase and vapor phase, and measuring the angle directly with a protractor; wherein the sample liquid contains sample and a sample diluent; and the sample diluent contains water, trifluoroacetic acid, and acetonitrile.