Patent ID: 6902909
Filing Date: 2005-06-07
Classification: C07K,C12M,C12N,C12P

Abstract:
1. A method for production of a human proteinaceous therapeutic molecule comprising: (a) identifying a mammalian cell line derived from a tissue that produces the proteinaceous therapeutic molecule in nature; (b) transfecting a plurality of cells from the mammalian cell line with a gene coding for the human proteinaceous therapeutic molecule; (c) cloning the transfected cells expressing the human proteinaceous therapeutic molecule; (d) expanding the cloned cells in a rotating cell culture system filled with a culture medium, wherein the rotating cell culture system provides a simulated micro-gravity environment for the expanding cloned cells and a membrane carrier assembly transverses a growth compartment, the membrane carrier comprising a support cylinder having a first end in communication with a fluid inlet and a second end in communication with a fluid outlet, a molecular weight cut-off membrane secured to an exterior surface of the support cylinder, and a medium circulation chamber between the exterior surface of the cylinder and an interior surface of the membrane, the chamber in fluid communication with the fluid inlet and the fluid outlet; (e) growing the cloned transfected cells in the rotating cell culture system, wherein the cloned cells synthesize the human protein and secrete the human protein into the culture medium in the rotating cell culture system: (f) separating a volume of culture medium from the expanded cloned cells; and (g) isolating a protein fraction from the volume of culture medium, wherein the protein fraction is rich in the proteinaceous therapeutic molecule.