Patent ID: 7169584
Filing Date: 2007-01-30
Classification: C12Q

Abstract:
1. In a method for a polymerase reaction, the method comprising (a) forming a reaction mixture comprising a thermostable polymerase, a nucleic acid polymer template, an anionic tracer dye compatible with the polymerase, and a primer, (b) creating a nucleic acid polymer product complementary to the nucleic acid polymer template by enzymatic reaction, (c) analyzing the product of the enzymatic reaction by an electrophoretic protocol, and (d) observing the tracer dye during the electrophoretic protocol without providing additional tracer beyond that which was included in the reaction mixture, the improvement comprising supplying the tracer dye to the reaction mixture as an aqueous reagent, the reagent comprising the anionic tracer dye, the thermostable polymerase, and a solute compatible with the reaction mixture to increase the physical density of the reagent, the reagent being free of the nucleic acid polymer template and the primer, and having an optical density of about 5 to about 500 at a visible wavelength of maximal tracer absorbance and a physical density of at least about 1.01 g/cm