Patent ID: 7749366
Filing Date: 2010-07-06
Classification: C12N

Abstract:
1. A device for the isolation and/or purification of nucleic acid molecules comprising at least two layers, a first layer being adapted to bind or inactivate inhibitors of the activity of reagents or enzymes used in nucleic acid manipulation, wherein said first layer comprises polyvinylpyrrolidone (PVP) or polyvinylpolypyrrolidone (PVPP), and wherein said first layer further comprises a sample loading means in an array in an upper portion of the first layer, defining an array of columns, each column being capable of isolating nucleic acid molecules, and a second layer being adapted to separate a plurality of nucleic acid molecules with respect to their size, and wherein said first layer is a first phase of a gel and said second layer is a second phase of said gel, wherein said first layer is arranged above the second layer, and wherein said second layer is substantially free of PVP when said first layer comprises PVP, and wherein said second layer is substantially free of PVPP when said first layer comprises PVPP.