Patent ID: 8512945
Filing Date: 2013-08-20
Classification: A61K,C12N,C12Q,C12Y

Abstract:
1. A method of cleaving an exogenous non-tRNA target RNA molecule in vitro comprising the steps of: (a) providing a first RNA molecule within a mammalian cell, the first RNA molecule being the exogenous non-tRNA target RNA molecule; (b) contacting the first RNA molecule with a second RNA molecule, wherein the second RNA molecule comprises a targeting region consisting of: whereby the first RNA molecule and the second RNA molecule form an RNA complex comprising a 3-nucleotide unpaired bulge-4-nucleotide paired helix-3-nucleotide unpaired bulge conformation, wherein one bulge has a guanine/adenine dinucleotide and the other bulge has a uracil/adenine dinucleotide; and (c) exposing the RNA complex to a heterologous archaeal