Patent ID: 8257758
Filing Date: 2012-09-04
Classification: A23J,A23L,A61K,A61P,Y02E

Abstract:
1. A purified amino-terminal polypeptide fragment of soy β-conglycinin α′ subunit, said fragment purified by a process comprising the steps of: (a) extracting a defatted ground soy with an aqueous solution of sodium bisulfite at slightly acidic pH to obtain a β-conglycinin-enriched soluble protein fraction; (b) precipitating the β-conglycinin protein fraction from step (a) by treating with ethanol; (c) purifying the precipitated fraction from step (b) by Metal Affinity Chromatography (MAC) under denaturing conditions, to isolate the α′ subunit; (d) precipitating the α′ subunit from step (c) with organic solvents; (e) enzymatically treating the α′ subunit from step (d) with chymotrypsin for a suitable time to produce enzymatically cleaved polypeptide fragments of the α′ subunit; (f) purifying the amino-terminal polypeptide fragment of the α′ subunit from step (e) by MAC.