Patent ID: 6291197
Filing Date: 2001-09-18
Classification: A61K,G01N

Abstract:
A recombinant bacteriophage expressing exogenous DNA encoding a parvovirus binding peptide having an amino acid sequence selected from the group consisting of:I. X.sub.1 -X.sub.2 -Cys-X.sub.3 -X.sub.4 -X.sub.5 -X.sub.6 -X.sub.7 -Cys-X.sub.8 -X.sub.9 (SEQ ID NO: 1),wherein X.sub.1 is Phe or Leu or is not present; X.sub.2 is Phe or Ser; X.sub.3 is Arg, Gln, Ser, His, Ala, Leu, or Gly; X.sub.4 is Phe, Tyr, Leu, or Trp; X.sub.5 is Trp or Phe; X.sub.6 is Tyr, Pro, or His; X.sub.7 is Gly, Asn, Ser, Phe, or Asp; X.sub.8 is His, Asp, Ser or Pro; X.sub.9 is Pro, Ala, Phe, His, or Asp or is not present,II. X.sub.10 -Phe-Cys-X.sub.11 -X.sub.12 -Trp-X.sub.13 -X.sub.14 -X.sub.15 -Cys-X.sub.16 -X.sub.17 (SEQ ID NO: 2),wherein X.sub.10 is His, Ala, or Phe; X.sub.11 is His, Trp, or Ser; X.sub.12 is Phe or Leu; X.sub.13 is Phe, Pro, or His; X.sub.14 is Gly or His; X.sub.15 is Gly or Asn; X.sub.16 Pro, Leu, or Asp; X.sub.17 is His or Asp,III. X.sub.18 -Cys-X.sub.19 -X.sub.20 -X.sub.21 -X.sub.22 -X.sub.23 -X.sub.24 -X.sub.25 -Cys-X.sub.26 (SEQ ID NO: 3),wherein X.sub.18 is Phe or Leu; X.sub.19 is Trp, His, Gln or Pro; X.sub.20 is Leu or Ala; X.sub.21 is Trp or His; X.sub.22 is Pro or Trp; X.sub.23 is Ser, Ala, Pro or Gln; X.sub.24 is Ser, His, or Phe; X.sub.25 is Asp, Ser, Gln or Trp; and X.sub.26 is Phe, His, Ala or Asp,wherein said binding peptide is displayed on the surface of said batereriophage.