Patent ID: 7629153
Filing Date: 2009-12-08
Classification: A61K,C12N

Abstract:
1. A method for producing a recombinant lentiviral vector comprising: (a) transfecting a cell with: (i) a packaging plasmid comprising a stuffer sequence and a cPPT/cTS region that has reduced replication activity relative to wild-type cPPT/cTS replication activity, wherein the packaging plasmid comprises a pol gene and an RRE, and the stuffer sequence is positioned between the pol gene and the RRE of the packaging plasmid; (ii) an expression plasmid, which carries an env gene; and (iii) a lentiviral transfer vector comprising a therapeutic transgene; to yield a producer cell; (b) culturing the producer cell in a medium; and (c) separating the producer cell from the medium to recover the recombinant lentiviral vector from the medium.