Patent ID: 6168952
Filing Date: 2001-01-02
Classification: A01H

Abstract:
A method for producing in vitro flowering orchid plants, said method comprising regeneration of orchid plants from rhizome in a medium containing cytokin or auxin followed by root excision in regenerated plants and culturing in a medium containing 10-50 .mu.M cytokin, 2-8 mM nitrogen and 3-9 mM phosphorus in a light/dark photoperiod, wherein the improvement comprises: (a) regenerating orchid plants from rhizome in a medium containing 2-5% w/v sugar and cytokinin (BA) 5-10 .mu.M or auxin (NAA) 10-20 .mu.M under an illumination regimen of about 14 to about 18 hours per day for about 3 to about 5 months; (b) root excision in the resulting regenerated orchid plants so that not more than about 0.1% to about 1.% of root biomass remains; (c) culturing the resulting plants from step (b) in a medium containing 3-4% w/v sugar, 20-50 .mu.M cytokin (6-benzylaminopurine), 2-6 mM total nitrogen, 2-8 mM total phosphorus for about 30 to about 100 days under an illumination regimen of about 14 to about 18 hours per day to produce induced plants comprising flower stalks with flower buds; and (d) culturing the induced plants in a medium contaiining 3-4% w/v sugar, 5-50 .mu.M 6-benzylaminopurine, 10-60 mM total nitrogen, 4-8 mM total phosphorus for about 20 to about 40 days under the illumination regimen of about 12 hours to about about 20 hours per day to develop said flowers.