Patent ID: 6524798
Filing Date: 2003-02-25
Classification: C12Q,G01N

Abstract:
A method of simultaneously determining a cell phenotype and genotype comprising:(a) contacting the cell with an antibody bound to a ligand, wherein the antibody is specific for an antigen suspected of being associated with the cell; (b) contacting the cell with polynucleotide probe under conditions that allow the probe to hybridize to a target sequence in a cellular nucleic acid, thereby forming a complex of the polynucleotide probe and cellular nucleic acid; (c) contacting the cell with a detectably labeled anti-ligand under conditions where the detectably labeled anti-ligand binds to the ligand; (d) detecting the presence of the labeled anti-ligand; (e) detecting the presence of the complex of the polynucleotide probe and cellular nucleic acid; and, (f) correlating the presence of the anti-ligand with the presence of the antigen in the cell thereby determining the cell phenotype, and correlating the presence of said complex of the polynucleotide probe and cellular nucleic acid with the presence of the target sequence in the cell, thereby determining the cell genotype; wherein said step (a) precedes step (b), and step (b) precedes step (c).