Patent ID: 7390654
Filing Date: 2008-06-24
Classification: A61K,C12N

Abstract:
1. In a plasmid selected from the group consisting of a pVAX1 vector, a pEBFP-N1 vector, a pECFP-N1 vector, a pEGFP-N1 vector, a pEGFP-N2 vector, a pEGFP-N3 vector, a pEYFP-N1 vector, a pEBFP-C1 vector, a pECFP-C1 vector, a pEGFP-C1 vector, a pEGFP-C2 vector, a pEGFP-C3 vector, a pEYFP-C1 vector, a pEGFP-F vector, a pCMS-EGFP vector, a pIRES2-EGFP vector, a pd2ECFP-N1 vector, a pd2EGFP-N1 vector, a pd2EYFP-N1 vector, a pd1 EGFP-N1 vector, a pCMV-Script vector, a pCMV-Tag vector, a pDual vector, a pBK-CMV vector, a pBK-RSV vector, a pTARGET vector, a pCI vector, and a pCI-Neo vector, said plasmid having a high copy number prokaryotic origin of replication and at least one eukaryotic control element located at a first distance in a direction of replication, wherein the first distance is less than about 1200 bp from the origin of replication, and wherein the plasmid produces a first population of liner replication side products, the improvement comprising: an increased distance in a direction of replication between the origin of replication and the control element, wherein the increased distance is at least about 1.5 kb, and wherein the improved plasmid produces a second population of linear replication side products, wherein the second population is substantially smaller than the first population.