Patent ID: 9109046
Filing Date: 2015-08-18
Classification: A61K,A61P,C12N,C12Y

Abstract:
1. A two-chain polypeptide comprising a first chain comprising (1) amino acid residues 1-105 of SEQ ID NO. 20 or (2) a first peptide having at least 95% sequence identity to amino acid residues 1-105 of SEQ ID NO. 20 and a second chain comprising (i) amino acid residues 112-346 of SEQ ID NO. 20 or (ii) a second peptide having at least 95% sequence identity to amino acid residues 112-346 of SEQ ID NO. 20, wherein the two-chain polypeptide (a) has reduced catalytic activity compared to wild-type factor Xa, (b) is capable of binding to a factor Xa inhibitor, and (c) does not assemble into a prothrombinase complex, and wherein the second chain does not include a β-peptide of wild-type factor Xa (amino acid residues 430-448 of SEQ ID NO. 3).