Patent ID: 6335461
Filing Date: 2002-01-01
Classification: A23L,C07K

Abstract:
A method of purifying N-[N-3,3-dimethylbutyl)-L-&agr;-aspartyl]-L-phenylalanine methyl ester, comprisingextracting said N-[N-3,3-dimethylbutyl)-L-&agr;-aspartyl]-L-phenylalanine methyl ester and N,N-di(3,3-dimethylbutyl)-APM from an aqueous solution comprising the N-[N-3,3-dimethylbutyl)-L-&agr;-aspartyl]-L-phenylalanine methyl ester, APM, and N,N-di (3,3-dimethylbutyl)-APM with an organic solvent, wherein the aqueous solution may be a slurry, wherein said organic solvent is selected from the group consisting of ethyl acetate, methyl acetate, toluene, tetrahydrofuran, acetonitrile, dimethoxyethane, isopropyl alcohol, ethyl alcohol, methyl alcohol, chloroform, acetone, methyl ethyl ketone, and mixtures thereof and further purifying the N-[N-3,3-dimethylbutyl)-L-&agr;-aspartyl]-L-phenylalanine methyl ester by a method selected from the group consisting of: (A) crystallizing said N-[N-3,3-dimethylbutyl)-L-&agr;-aspartyl]-L-phenylalanine methyl ester; (B) subjecting said N-[N-3,3-dimethylbutyl)-L-&agr;-aspartyl]-L-phenylalanine methyl ester to chromatography; (C) extracting said N,N-di(3,3-dimethyl)-APM and (D) absorbing said N-[N-3,3-dimethylbutyl)-L-&agr;-aspartyl]-L-phenylalanine methyl ester on activated charcoal.