Patent ID: 6114296
Filing Date: 2000-09-05
Classification: C11D,C12N,C12Y,D06M,D06P

Abstract:
A cellulase variant of a microbial parent cellulase having a catalytically active domain classified in family 45, said variant comprising a cellulose binding domain (CBD), a catalytically active domain (CAD) and a region linking the cellulose binding domain and catalytically active domain (the linking region), wherein said variant comprises a substitution of one or more amino acid residues at a position corresponding to a position in SEQ ID NO:10 selected from the group consisting of:(a) positions 4, 5, 6, 7, 8, 10, 11, 12, 13, 15, 18, 20, 21, 44, 45, 48, 74, 82, 90, 110, 114, 117, 119, 121, 128, 131, 132, 147, 176, 178 or 179, wherein the substitution is predicted to alter the surface conformation of the active site cleft;(b) positions 111, 112, 113, 114, 115, 116, 117, 118 or 119, wherein the substitution is predicted to alter the hydrogen bonding properties of the flexible loop region;(c) positions 9, 14, 28, 37, 55, 58, 59, 60, 63, 72, 73, 78, 109, 118, 123, 129, 131, 132, 133, 136, 142, 145, 146, 158, 163, 176, 179, 186 or 196, wherein the substitution is predicted to alter the surface conformation of a channel;(d) positions 2, 13, 20, 44, 65, 66, 67, 90, 95, 96, 100, 102, 103, 175, 176, 178, 180, 183 or 185, wherein the substitution is predicted to alter the positive electrostatic charge of a positively charged surface region;(e) positions 55, 74, 90 or 123, wherein the substitution is predicted to alter the negative charge of a cleft;(f) positions 37, 62, 63, 78, 118, 129, 131, 133, 136, 142, 146, 158, 162, 163, 175, 176, 179, 186 or 196, wherein the substitution is predicted to alter the sensitivity of the variant to anionic surfactants;(g) positions 240 and 241 in the linking region;(h) positions 251, 252, 253, 254, 255, 261, 262, 265, 268, 269, 274, 275, 276, 277, 278, 279, 280, 281, or 282 in the cellulose binding domain; and(i) combinations of any of the foregoing.