Patent ID: 6524796
Filing Date: 2003-02-25
Classification: A61K,C07K

Abstract:
A method of screening for a protein that regulates promoter activity of a human p27Kip1 promoter DNA, said promoter DNA containing at least a substantial part of a region extending from position 2795 to 3133 of SEQ I) NO:1, wherein said promoter DNA retains the ability to promote the expression of a human p27Kip1 gene, said method comprising a step selected from the group consisting of:(a) bringing a test sample, said sample containing a test protein, into contact with said promoter DNA and selecting the protein that binds to said promoter DNA; (b) introducing a test DNA coding for a test protein into cells carrying a reporter gene coding for an expression product fused downstream of said promoter DNA, testing for the presence of said reporter gene expression product, and selecting the test protein that regulates reporter gene expression; and (c) bringing a test sample, said sample containing a test protein, into contact with cells carrying a reporter gene coding for an expression product fused downstream of said promoter DNA, testing for the presence of said reporter gene expression product, and selecting the test protein that regulates reporter gene expression.