Patent ID: 8329412
Filing Date: 2012-12-11
Classification: C07K,C12N,C12Q,G01N

Abstract:
1. A method for determining the presence or absence and/or quantity of NAE1-beta, or NAE1-beta complex in a biological sample, the method comprising providing a detectable Neural precursor cell-Expressed Developmentally Down regulated 8 (NEDD8) to a biological sample, allowing the detectable NEDD8 to bind to NAE1-beta, or NAE1-beta complex, if any is present in the biological sample, and detecting the presence or absence and/or quantity of a complex of the detectable and NAE1-beta, or NAE1-beta complex, wherein the NAE1-beta or NAE1-beta complex comprises an NAE1-beta polypeptide selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence which is at least 95% identical to the amino acid sequence of SEQ ID NO:2, and (b) a polypeptide encoded by a nucleic acid comprising a nucleotide sequence which is at least 95% identical to SEQ ID NO:1; wherein the polypeptide has an activity selected from the group consisting of: ability to form an adenylate intermediate with Neural precursor cell-Expressed Developmentally Down regulated 8 (NEDD8), ability to form a thioester linkage with NEDD8, and activity as a dominant negative mutant of NAE1-beta.