Patent ID: 9017970
Filing Date: 2015-04-28
Classification: A61P,C12N,C12P,C12Q,Y10T

Abstract:
1. A method for obtaining, isolating, or purifying capped RNA in a sample that contains said capped RNA and at least one uncapped RNA, the method comprising the steps of: (1) providing: (i) a sample that contains capped RNA and at least one uncapped RNA that has a 5′ triphosphate group or a 5′ diphosphate group, (ii) an RNA polyphosphatase that is active in the presence of EDTA and is inhibited in the presence of 1 mM or greater Mg (2) contacting the sample with the RNA polyphosphatase thereby forming a first reaction mixture such that the RNA that has a 5′ triphosphate group or a 5′ diphosphate group is converted to RNA that has a 5′ monophosphate group in said first reaction mixture; and (3) contacting said first reaction mixture with said 5′ exoribonuclease under conditions such that said RNA that has a 5′ monophosphate group in said first reaction mixture is digested by the 5′ exoribonuclease, but said capped RNA in said first reaction mixture is not digested by the 5′ exoribonuclease, thereby obtaining, isolating, or purifying said capped RNA.