Patent ID: 8951499
Filing Date: 2015-02-10
Classification: A61K,C07D,G01N

Abstract:
1. A reagent for conjugation to a biomolecule having the formula: wherein the trifunctional cross-linking moiety is coupled to a) the affinity ligand via linker 1, wherein the affinity ligand is selected from the group consisting of biotin, norbiotin, homobiotin, oxybiotin, iminobiotin, desthiobiotin, diaminobiotin, biotin sulfoxide, and biotin sulfone, and wherein linker 1 comprises a carboxylate group or an N-methyl group effective to inhibit biotinidase activity, b) an effector agent via linker 2, said effector agent exerting its effect on cells, tissues and/or humorous molecules in vivo or ex vivo, and c) the biomolecule reactive moiety via a linker 3, said moiety being capable of forming a bond between the reagent and the biomolecule.