Patent ID: 8624014
Filing Date: 2014-01-07
Classification: C12N,C40B,G16B

Abstract:
1. A composition comprising oligonucleotides, each oligonucleotide in the composition either being free of cytosine residues and comprising guanosine residues or being free of guanosine residues and comprising cytosine residues, wherein for each oligonucleotide that comprises cytosine residues, the cytosine residues are separated by between one and six non-cytosine residues, and for each oligonucleotide that comprises guanosine residues, the guanosine residues are separated by between one and six non-guanosine residues, wherein the number of cytosine and guanosine residues present in each oligonucleotide in the composition does not exceed L/4 where L is the number of bases in the oligonucleotide, wherein the length of each oligonucleotide in the composition differs by no more than five bases from the average length of all oligonucleotides in the composition, wherein each oligonucleotide in the composition contains no more than 4 contiguous identical nucleotides, wherein the number of guanosine and cytosine residues present in each oligonucleotide in the composition does not vary from the average number of guanosine and cytosine residues present in all other oligonucleotides of the composition by more than one, and when each oligonucleotide of the composition is exposed to hybridization conditions comprising 0.2M NaCl, 0.1M Tris, 0.08% Triton X-100, pH 8.0 at 37° C., the degree of cross-hybridization between the oligonucleotide and any complement of a different oligonucleotide of the composition does not exceed 30% of the degree of hybridization between the oligonucleotide and an oligonucleotide fully complementary thereto.