Patent ID: 6489109
Filing Date: 2002-12-03
Classification: C12Q

Abstract:
A method of determining the chromosomal location of an expressed nucleic acid sequence, the method comprising:a) providing a first nucleic acid sample from a hybrid cell, wherein the hybrid cell includes an endogenous genome and at least a portion of an exogenous genome, wherein the first nucleic acid sample is a cDNA population derived from a population of RNA molecules isolated from said hybrid cell; b) probing said sample with one or more endonuclease enzymes, wherein each of said enzymes recognize a different target nucleotide subsequence or a different set of target nucleotide subsequences; c) generating one or more output signals from said sample probed by said enzyme, each output signal being produced from a nucleic acid in said sample by recognition of one or more target nucleotide subsequences in said nucleic acid by said enzyme and comprising a representation of (i) the length between occurrences of target nucleotide subsequences in said nucleic acid, and (ii) the identities of said target nucleotide subsequences in said nucleic acid or the identities of said sets of target nucleotide subsequences among which are included the target nucleotide subsequences in said nucleic acid, and measuring the level of said one or more output signals; and d) comparing the output signals from said hybrid cell sample with the output signals of a second sample that is processed as in steps (b) and (c), wherein the second sample is obtained from a reference cell, wherein said reference cell comprises the endogenous genome of said hybrid cell but does not include the exogenous genome present in said hybrid cell; whereby a nucleic acid present at increased levels in the hybrid cell relative to the reference cell indicates that said nucleic acid is encoded by a gene present in the exogenous genome present in said hybrid cell, thereby determining the chromosomal location of an expressed nucleic acid sequence.