Patent ID: 8993294
Filing Date: 2015-03-31
Classification: B01D,C07K

Abstract:
1. A method for recovering a protein of interest from a culture solution using cross-flow membrane filtration, comprising: subjecting a culture solution comprising a protein of interest to cross-flow membrane filtration using a first cross-flow membrane under a first set of conditions that cause the protein of interest to be retained as retentate to allow purification, concentration, and/or buffer exchange of the protein of interest; exposing the protein of interest retained by the first cross-flow membrane to a second cross-flow membrane under a second set of conditions under which the apparent molecular weight of the protein of interest is reduced such that it passes through the second membrane as filtrate to allow purification and/or recovery of the protein of interest; wherein the first and the second cross-flow membrane are the same type of cross-flow filtration membrane and have a pore size that provides a molecular weight cut-off that is greater than the actual molecular weight of the protein of interest; characterized in that the first set of conditions causes the protein of interest to form multimers, to aggregate, to crystallize, to precipitate, to form a gel, or combinations thereof.