Patent ID: 6884576
Filing Date: 2005-04-26
Classification: C12Q,G01N

Abstract:
1. A method for detecting HIV drug resistance in a sample, comprising: taking a culture of recombinant cells in which at least one of the recombinant cells comprises (a) a reporter sequence comprising a reporter gene whose expression is regulated by a protein specific to HIV, and (b) a heterologous sequence which encodes CD4 and one or more additional cell surface receptors, wherein the heterologous sequence expresses CD4 and the one or more additional cell surface receptors at elevated levels as compared to the cell in the absence of expression by the heterologous sequence such that productive infection of the recombinant cell by the HIV is achieved, which is defined by HIV viral replication and the infection of non-infected cells in the culture of the recombinant cells; contacting the cell culture with a sample containing HIV; adding one or more anti-HIV agents to the cell culture either before or after contacting the cell culture with the sample; and detecting a change in a level of expression of the reporter gene in the cells.