Patent ID: 6030819
Filing Date: 2000-02-29
Classification: C12N,C12P,Y10S

Abstract:
A method of producing 4-hydroxybenzoic acid, comprising:a) providing E. coli cells;b) exposing the E. coli cells to an aromatic amino acid antimetabolite;c) performing a first isolation to isolate the E. coli cells which survive the exposure to the antimetabolite;d) exposing the E. coli cells isolated in the first isolation to para-aminobenzoic acid;e) performing a second isolation to isolate the E. coli cells which survive the exposure to para-aminobenzoic acid;f) providing a plasmid which is capable of expressing the ubiC gene product;g) transforming the E. coli cells isolated in the second isolation with the plasmid; andh) allowing the transformants to ferment in the presence of glucose.