Patent ID: 7223568
Filing Date: 2007-05-29
Classification: C12Q

Abstract:
1. A method for determining a nucleotide sequence of a single nucleic acid molecule, which comprises: (a) immobilizing a nucleic acid molecule onto the surface of a solid; (b) annealing a primer to said nucleic acid molecule, wherein said primer has a sequence complementary to a part of a sequence of the nucleic acid molecule; (c) providing a solution which contains a DNA polymerase and only one type of dye-labeled dNTP, where N is A, T or U, G or C, or an RNA polymerase and only one type of dye-labeled NTP, where N is A, U, G or C, to said immobilized nucleic acid molecule, and allowing the dye-labeled dNTP or NTP to react with the 3′ end of said primer, whereby the dye-labeled dNTP or NTP, which forms a base-pair with a base in the nucleic acid molecule at a position where the dye-labeled dNTP or NTP reacts with the 3′ end of said primer and is bound to the primer by action of the polymerase; (d) detecting a bound, dye-labeled dNTP or NTP; (e) disrupting the dye molecule of the bound, dye-labeled dNTP or NTP; (f) repeating (c) to (e) while changing the type of dye-labeled dNTP or NTP in turn, to sequentially bind dNTPs or NTPs which forms a base-pair with the nucleotides of the nucleic acid molecule; and (g) determining a nucleotide sequence of the nucleic acid molecule based on the types of the sequentially bound dNTPs or NTPs.