Patent ID: 7244570
Filing Date: 2007-07-17
Classification: C12Q

Abstract:
1. A method of identifying a nucleic acid molecule suitable for use in a probe for detecting the presence of one or more of a family of nucleic acid molecules, comprising the steps of: (a) providing the family of first nucleic acid molecules wherein each member of the family is related to all other members of the family by consensus sequence; (b) providing a second nucleic acid molecule having a sequence complementary to the consensus sequence; and (c) determining the ability of the second nucleic acid molecule to form a duplex with each member of the family in the presence of a first ligand known to affect duplex formation of nucleic acid molecules at a plurality of concentrations of the first ligand, wherein the nucleic acid molecule suitable for use in a probe is identified at a ligand concentration at which the second nucleic acid molecule has substantially the same ability to form a duplex with each member of the family.