Patent ID: 9133525
Filing Date: 2015-09-15
Classification: A61K,C12N,C12Q,Y02A

Abstract:
1. A method for detecting an intracellular microbiological organism having a 16s rRNA gene and capable of passing through a 0.45 micron filter, selectively associated with red blood cells, comprising: amplifying genetic material consisting essentially of material extracted from the intracellular space of red blood cells of a subject with at least one pair of primers selected from the group consisting of at least one of: under conditions suitable for amplification of nucleic acids of the intracellular microbiological organism by said at least one pair of primers; quantitatively determining an amplification of genetic material from said amplifying; and producing at least one signal indicating a detecting of said intracellular microbiological organism when said genetic material is determined to be amplified.