Patent ID: 7410760
Filing Date: 2008-08-12
Classification: C12Q

Abstract:
1. A method for detecting a control polynucleotide in a multiplex amplification reaction, the method comprising, providing: amplifying the single-stranded control polynucleotide by polymerase chain reaction using said first primer pair, wherein said second primer is sufficiently complementary to said second primer binding sequence to allow for template-dependent extension of said second primer, and wherein said nucleic acid probe is sufficiently complementary to said probe binding site to allow for detection of said single-stranded polynucleotide sequence; and detecting the presence or absence of the amplified control polynucleotide by detecting hybridization of the nucleic acid probe to the probe-binding sequence in the amplified single-stranded control polynucleotide, thereby detecting the control polynucleotide in a multiplex amplification reaction.