Patent ID: 6811968
Filing Date: 2004-11-02
Classification: B01D,B01J,B01L,B82Y,C12Q,C40B,G01N,G06T,Y10S

Abstract:
A method of monitoring chemotaxis or chemoinvasion comprising:providing a device for monitoring chemotaxis having: a support member; a top member mounted to the support member by being placed in substantially fluid-tight, conformal contact with the support member, wherein the support member and the top member are configured such that they together define a discrete assay chamber including: a first well region including at least one first well, the at least one first well being configured to receive a soluble test substance therein; a second well region including at least one second well horizontally offset with respect to the first well region in a test orientation of the device; the at least one second well being configured to receive a sample comprising cells therein; and a channel region including at least one channel connecting the first well region and the second well region; placing the soluble test substance in the at least one first well; forming a solution concentration gradient along a longitudinal axis of the chamber; placing a first sample comprising cells in the at least one second well; and monitoring chemotaxis of the cells.