Patent ID: 8426147
Filing Date: 2013-04-23
Classification: A01K,A61P,C07K,C12Q,G01N

Abstract:
1. A method of identifying a p21 pathway modulating agent, said method comprising the steps of: (a) providing a first assay system comprising a Retinoic Acid Receptor Related Orphan Receptor (ROR) nucleic acid, wherein the nucleic acid is selected from the group consisting of SEQ ID NOs: 1-15, a nucleic acid encoding the polypeptide of SEQ ID NO: 16, 17, or 18, and a nucleic acid encoding amino acids 104-179 of SEQ ID NO: 16, amino acids 361-544 of SEQ ID NO: 16, amino acids 8-83 of SEQ ID NO: 17, amino acids 267-450 of SEQ ID NO: 17, amino acids 29-104 of SEQ ID NO: 18, or amino acids 325-506 of SEQ ID NO: 18; (b) contacting the first assay system with a test agent that modulates the expression of the ROR nucleic acid of step (a); (c) identifying the test agent as a p21 pathway modulating agent by detecting a change in the activity of the first assay system in the presence or absence of the test agent of step (b); (d) providing a second assay system comprising cultured cells expressing ROR, wherein the second assay is capable of detecting a change in the p21 pathway; (e) contacting the second assay system with the test agent of (b); and (f) confirming the test agent as a p21 pathway modulating agent by detecting a change in the second assay system in the presence or absence of the test agent.