Patent ID: 9193953
Filing Date: 2015-11-24
Classification: A61P,C12N

Abstract:
1. A method for-generating a collection of differentiated cells comprising culturing cells from a sample comprising stem cells, progenitor cells or both, from human postembryonic tissue in an expansion medium comprising desulphated glycosaminoglycan (GAG), three or more of TPO, FLT-3L, SCF, IL-7, IL-3, IL-15, and in addition three or more of GM-CSF, G-CSF, LIF, MIP-1-α and IL 6 followed by culturing the cells in an expansion and differentiation medium comprising a collection of cytokines, desulphated glycosaminoglycan (GAG) and human serum, wherein said collection of cytokines comprises three or more of TPO, FLT-3L, SCF, IL-7, IL-2, IL-15, and in addition three or more of GM-CSF, G-CSF, LIF, MIP-1-α and IL-6, wherein the cultured isolated cells are expanded and differentiated to generate the collection of differentiated cells.