Patent ID: 6562567
Filing Date: 2003-05-13
Classification: C12Q

Abstract:
A method for detecting a first target nucleic acid molecule and a second target nucleic acid molecule in a collection of at least two nucleic acid molecules having different nucleotide sequences, the method comprising:providing a first oligonucleotide covalently linked to a first removable tag by a first photocleavable linker, and a second oligonucleotide covalently linked to a second removable tag by a second photocleavable linker, wherein the first and second photocleavable linkers are cleavable at different wavelengths; contacting the first oligonucleotide and the second oligonucleotide with the collection of nucleic acid molecules in a single reaction under conditions which permit hybridization of the first oligonucleotide and the second oligonucleotide to complementary sequences in the collection of nucleic acid molecules, thereby forming a mixture of hybridized and non-hybridized oligonucleotides; separating hybridized oligonucleotides from non-hybridized oligonucleotides; removing the first tag from hybridized first oligonucleotides by irradiating the mixture of hybridized and non-hybridized oligonucleotides at a first wavelength, thereby cleavinthe first photocleavable linker; identifying the first tag by its known chemical property, thereby detecting the first target nucleic acid molecule; removing the second tag from hybridized second oligonucleotides by irradiating the mixture of hybridized and non-hybridized oligonucleotides at a second wavelength, thereby cleaving the second photocleavable linker; and identifying the second tag by its known chemical property, thereby detecting the second target nucleic acid molecule in the collection of nucleic acid molecules.