Patent ID: 6485922
Filing Date: 2002-11-26
Classification: C07K,C12Q

Abstract:
A method for detecting compounds of interest which act as inverse agonists of LPA in its interaction with EDG-2, comprising the steps ofa) exposing a compound and LPA to host fungal cells transgenically expressing the EDG-2 receptor (SEQ ID NO. 20), wherein the EDG-2 receptor is coupled to a response pathway through an interaction with the endogenous G-protein of the host fungal cell, under conditions and for a time sufficient to allow interaction of LPA with the EDG-2 receptor and an associated response through the pathway; and b) detecting an increase or a decrease in a product of the response pathway resulting from the interaction of the compound with the EDG-2 receptor, relative to the absence of the tested compound, and therefrom determining the presence of an antagonist or an allosteric modulator.