Patent ID: 8679812
Filing Date: 2014-03-25
Classification: C07K,G01N

Abstract:
1. A method for extracting a Staphylococcus aureus antigen which comprises: contacting an extraction reagent with a pH of no higher than 5.0, containing one or more acids selected from among hydrochloric acid, acetic acid, citric acid, phosphoric acid, sulfuric acid and nitric acid with Staphylococcus aureus in a specimen, to extract a Staphylococcus aureus antigen comprising a methicillin-resistant Staphylococcus aureus antigen and/or a methicillin-sensitive Staphylococcus aureus antigen, from the Staphylococcus aureus in a specimen, wherein the methicillin-resistant Staphylococcus aureus antigen is penicillin-binding protein 2′ and the methicillin-sensitive Staphylococcus aureus antigen is penicillin-binding protein 2, and wherein, when the Staphylococcus aureus is a methicillin-resistant Staphylococcus aureus, then both the penicillin-binding protein 2′ and the penicillin binding protein 2 are extracted, and when the Staphylococcus aureus is a methicillin-sensitive Staphylococcus aureus , then the penicillin-binding protein 2 is extracted, while the penicillin-binding protein 2′ is not extracted, wherein the extraction reagent contains one or more surfactants selected from the group consisting of an anionic surfactant, zwitterionic surfactant, cationic surfactant and nonionic surfactant.