Patent ID: 7803582
Filing Date: 2010-09-28
Classification: A61K,A61P,C12N,C12Y

Abstract:
1. A method for producing a recombinant vector from a vector-producing cell line, wherein the vector comprising mutant Apolipoprotein B mRNA-editing enzyme-catalytic polypeptide-like 3G (A3G) genes for delivering said mutant A3G genes into human target cells comprising the steps of: a) establishing a cell line by culturing a cell line in a culture medium, b) transfecting the cell line with supporting plasmids containing a Vif gene, wherein said Vif gene expresses a Vif protein in the cell line, c) selecting the cell line stably expressing the Vif protein, d) infecting the cell line of step c) with a recombinant vector comprising (i) a gene expression block including a mutant A3G gene and (ii) a group of elements from a modified lentiviral vector including lentiviral regions of packaging signal (ψ, psi), long-term repeats (LTRs), Rev responsive element (RRE), and primer binding site (PBS); wherein said mutant A3G gene is operably linked to the packaging signal (ψ, psi), LTRs, RRE, and PBS, e) selecting the cell lines stably producing the recombinant vector, and f) expressing envelope proteins in the selected cell line of step e) and harvesting the recombinant vector from the culture medium.