Patent ID: 6232060
Filing Date: 2001-05-15
Classification: C12Q

Abstract:
A method for assaying a candidate compound for cytoprotective property against mitochondrial inhibitors, said method comprising:combining oxidative WIF-B hepatoma derived cells in a first assay medium comprising a mitochondrial inhibitor and combining oxidative WIF-B hepatoma derived cells in a second assay medium, wherein said second assay medium comprises said first assay medium, said mitochondrial inhibitor and a candidate compound;incubating said WIF-B cells in said first assay medium and said WIF-B cells in said second assay medium; andcomparing amount of lactate dehydrogenase (LDH) in the second assay medium with amount of LDH in the first assay medium,whereby a reduction in the amount of LDH in the second assay medium as compared to the amount of LDH in the first assay medium indicates the candidate compound has a cytoprotective property.