Patent ID: 6344596
Filing Date: 2002-02-05
Classification: A01K,A61K,A61P,C12N,C12Y

Abstract:
A non-human transgenic mammal containing an exogenous DNA molecule that is stably integrated in its genome, wherein said exogenous DNA molecule comprises:(a) 5â€² regulatory sequences of a mammary gland-specific gene including a promoter; (b) a Factor IX-encoding DNA sequence that encodes a signal sequence, a Factor IX pro-sequence, and a Factor IX sequence in a 5â€² to 3â€² direction, wherein said signal sequence is effective in directing the secretion of said Factor IX into the milk of said transgenic mammal and wherein said Factor IX sequence lacks the complete 5â€²-untranslated and 3â€²-untranslated regions of the Factor IX gene; and (c) 3â€² regulatory sequences from a mammary gland-specific gene or 3â€² regulatory sequences active in a mammary gland, wherein said 5â€² and said 3â€² regulatory sequences are operatively linked to said Factor IX-encoding DNA sequence, and wherein said promoter is selected from the group consisting of whey acidic protein (WAP) promoter, &agr;-casein promoter, &bgr;-casein promoter, kappa-casein promoter, &agr;-lactalbumin promoter and &bgr;-lactoglobulin promoter.