Patent ID: 7247435
Filing Date: 2007-07-24
Classification: C12Q,G01N

Abstract:
1. A method of determining cAMP content or an adenylate cyclase activity in a biological sample comprising the following steps: Cleaning Reaction: combining a biological sample with effective amounts of apyrase, alkaline phosphatase and adenosine deaminase without 5′-nucleotidase to enzymatically remove endogenous non-cyclic adenine nucleotides other than cAMP, and endogenous glucose-6-phosphate; Converting Reaction: enzymatically converting cAMP in the biological sample into ATP; and Detecting Reaction: enzymatically converting ATP into fructose-6-phosphate which is then enzymatically converted into 6-phosphogluconolactone and NADPH and determining a concentration of NADPH without the use of radioactive agents.