Patent ID: 9187731
Filing Date: 2015-11-17
Classification: A61K,A61P,C07K,C12N

Abstract:
1. A replication-competent Porcine Reproductive and Respiratory Syndrome (PRRS) virus comprising a deletion of at least three consecutive nucleotide triplets in the gene sequence encoding the N terminal domain (NTD) of the nsp1β subunit of the nsp1 protein of said virus, wherein the NTD starts with the N-terminus of nsp1β of amino acid sequence SXXY (SEQ ID NO: 60) and ends with the serine (S) residue within the amino acid sequence SFP (SEQ ID NO: 62), wherein said deletion of three consecutive nucleotide triplets comprises the first encoded arginine residue (R) located at least 21 amino acid residues located in the C-terminal direction from the NTD amino acid residue of nsp1β and two amino acid residues flanking said R residue in the N-terminal direction, and wherein the modified PRRS virus induces production and secretion of interferon type I by a cell infected with said virus.