Patent ID: 9145588
Filing Date: 2015-09-29
Classification: C07K,C12Q,C40B

Abstract:
1. A method for producing a population of antibodies which bind to an antigen, said method comprising: a) providing a population of B cells from one transgenic mouse immunized with said antigen, b) obtaining essentially all of the nucleic acids encoding antibody heavy chain variable (VH) regions from essentially all of said B cells, c) obtaining nucleotide sequences of essentially all obtained nucleic acids of step b), d) determining the frequency of nucleotide sequences from step c) which encode VH regions with unique HCDR3 amino acid sequences, e) selecting nucleic acids encoding VH regions comprising frequently occurring HCDR3 amino acid sequences identified in step d), f) providing host cells, wherein each host cell comprises at least one vector comprising a nucleotide sequence encoding at least one VH region selected in step e) wherein each said host cell expresses the single rearranged human VL nucleic acid of step a) and at least one VH region selected in step e), g) culturing said host cells from step f) and allowing for expression of said VL and VH regions, and h) obtaining said population of antibodies from the cultured host cells of step g) which bind said antigen.