Patent ID: 6623920
Filing Date: 2003-09-23
Classification: C12Q

Abstract:
A method of detecting HIV-1 nucleic acid in a biological sample, comprising the steps of:providing a biological sample containing HIV-1 nucleic acid; contacting the biological sample with at least one capture oligomer comprising a base sequence that hybridizes specifically to a target region in LTR or pol sequences of HIV-1 nucleic acid, thus forming a capture oligomer:HIV-1 nucleic acid complex; separating the capture oligomer:HIV-1 nucleic acid complex from the biological sample; then amplifying the LTR or pol sequences, or a cDNA made therefrom, using a nucleic acid polymerase in vitro to produce an amplified product; and detecting the amplified product using a labeled detection probe that hybridizes specifically with the amplified product, thereby indicating presence of the HIV-1 nucleic acid in the biological sample.