Patent ID: 8962813
Filing Date: 2015-02-24
Classification: A61P,C07K

Abstract:
1. A method of purifying a factor for supporting wound healing selected from the group consisting of hepatocyte growth factor (HGF), platelet derived growth factor (PDGF), epidermal growth factor (EGF), transforming growth factor alpha (TGF-α), transforming growth factor beta (TGF-β), and insulin like growth factor (IGF-I) from a source containing said factor, wherein all purification steps of the method are performed in the presence of antithrombin III (AT-III) and the method comprises: (i) thawing a frozen source containing the factor for supporting wound healing, removing a precipitate of the thawed source, and further processing a supernatant of the thawed source containing the factor for supporting wound healing; (ii) contacting a solution containing the supernatant and AT-III with a heparin-affinity chromatography material; (iii) separating the solution from the heparin-affinity chromatography material and contacting the heparin-affinity chromatography material with a desorption buffer having an ionic strength sufficient to desorb the factor for supporting wound healing from the heparin-affinity chromatography material; and (iv) collecting the desorption buffer containing the factor for supporting wound healing, AT-III, and histidine-rich glycoprotein (HRGP).