Patent ID: 8849581
Filing Date: 2014-09-30
Classification: G01N

Abstract:
1. A method for evaluating an action of one or more compounds on a molecular flux rate through an adipose triglyceride metabolic pathway in a living system, said method comprising: a) administering a first isotope-labeled substrate to a first living system, not exposed to said one or more compounds, for a period of time sufficient for said first isotope-labeled substrate to enter into and label at least one glycerol or fatty acid derived from triglyceride to produce at least one first isotope-labeled glycerol or first isotope-labeled fatty acid derived from triglyceride within said first living system; b) obtaining one or more samples from said first living system, wherein said one or more samples comprise said at least one first isotope-labeled glycerol or first isotope-labeled fatty acid derived from triglyceride; c) measuring an isotopic content, rate of incorporation, and/or pattern or rate of change in isotopic content and/or pattern of isotope labeling of said at least one first isotope-labeled glycerol or first isotope-labeled fatty acid derived from triglyceride; d) calculating a molecular flux rate through said adipose triglyceride metabolic pathway based on the isotopic content, rate of incorporation, and/or pattern or rate of change of isotopic content and/or pattern of isotopic labeling in said at least one first isotope-labeled glycerol or first isotope-labeled fatty acid derived from triglyceride; e) exposing a second living system to said one or more compounds; f) administering a second isotope-labeled substrate to said second living system for a period of time sufficient for said second isotope-labeled substrate to enter into and label at least one glycerol or fatty acid derived from triglyceride to produce at least one second isotope-labeled glycerol or second isotope-labeled fatty acid derived from triglyceride; g) obtaining one or more samples from said second living system, wherein said one or more samples comprise said at least one second isotope-labeled glycerol or second isotope-labeled fatty acid derived from triglyceride; h) measuring an isotopic content, rate of incorporation, and/or pattern or rate of change in isotopic content and/or pattern of isotope labeling of said at least one second isotope-labeled glycerol or second isotope-labeled fatty acid derived from triglyceride; i) calculating a molecular flux rate through said adipose triglyceride metabolic pathway in said second living system based on the isotopic content, rate of incorporation, and/or pattern or rate of change in isotopic content and/or pattern of isotope labeling of said at least one second isotope-labeled glycerol or second isotope-labeled fatty acid derived from triglyceride; and j) comparing said molecular flux rate through said adipose triglyceride metabolic pathway in said first living system to said molecular flux rate through said adipose triglyceride metabolic pathway in said second living system to evaluate the action of said one or more compounds on said molecular flux rate through the adipose triglyceride metabolic pathway in said second living system.