Patent ID: 8137940
Filing Date: 2012-03-20
Classification: C12N,C12P

Abstract:
1. A process of using a microorganism, wherein said microorganism is a Gluconobacter , for the production of biomass, wherein the gene encoding NADP-dependent glucose dehydrogenase has been underexpressed or disrupted, said gene comprising a polynucleotide selected from the group consisting of: (i) nucleotide sequences encoding a polypeptide comprising SQ ID NO:2; (ii) nucleotide sequences comprising SEQ ID NO:1; (iii) nucleotide sequences the complementary strand of which hybridizes under highly stringent conditions to a nucleotide sequence as defined in (i) or (ii) and which encode an NADP-dependent glucose dehydrogenase, wherein said highly stringent conditions comprise hybridization at about 42° C. for about 2 to 4 days followed by one or more washes in 2×SSC, 0. (iv) nucleotide sequences which are at least 95% identical to a polynucleotide as defined in (i) or (ii) and which encode an NADP-dependent glucose dehydrogenase; or the complementary strand of such a polynucleotide; wherein the amount of biomass produced is increased by at least 130% compared to a microorganism having an intact gene as defined above.