Patent ID: 6803234
Filing Date: 2004-10-12
Classification: A61K,A61P,C12N

Abstract:
An in vitro method of delivering a nucleic acid of interest to a primary human chondrocyte, comprising:providing a recombinant adenovirus having a tropism for primary human chondrocytes, said recombinant adenovirus comprising: a nucleic acid of interest operatively linked to a promoter, wherein said nucleic acid of interest encodes at least one amino acid sequence that inhibits cartilage disease progression, at least one amino acid sequence that counteracts the loss of cartilage, or a combination thereof; a deletion in a gene encoding a fiber protein; and a nucleic acid replacing the deletion in the gene of the fiber protein, said nucleic acid encoding at least a part of a fiber protein of a B-type adenovirus, and wherein said at least a part of the fiber protein of the B-type adenovirus has a tropism for primary human chondrocytes; and infecting a primary human chondrocyte in vitro with said recombinant adenovirus, such that said nucleic acid of interest is delivered to said primary human chondrocyte.