Patent ID: 9194778
Filing Date: 2015-11-24
Classification: G01N

Abstract:
1. A method of separating cells, the method comprising: incubating a sample comprising target cells with two or more antibodies or antigen binding fragments thereof that specifically bind cell-surface proteins of the target cell, wherein the two or more antibodies or antigen binding fragments thereof are from two or more different host species, to prepare a first complex of a target cell and at least one of the two or more antibodies or antigen binding fragments thereof; incubating the first complex with a second complex comprising at least one anti-immunoglobulin antibody linker that specifically binds to an antibody or antigen binding fragment thereof of the first complex, wherein the at least one anti-immunoglobulin antibody linker is bound to a solid phase, and wherein incubating the first complex with the second complex provides a third complex comprising the target cell, the at least one antibody or the antigen binding fragment of the first complex, the anti-immunoglobulin antibody linker, and the solid phase, and separating the target cell from the third complex.