Patent ID: 6265634
Filing Date: 2001-07-24
Classification: C12N

Abstract:
A polyribozyme which has endoribonuclease activity and inactivates a capsid protein gene of a Cucumber Mosaic Virus where the polyribozyme comprises:a) a first ribozyme targeted to nucleotide 84 of the Cucumber Mosaic Virus capsid protein gene (SEQ ID NO: 5), or the corresponding site in a transcript of the capsid protein gene, or a replication intermediate of the virus; andb) a second ribozyme targeted to nucleotide 108 of the Cucumber Mosaic Virus capsid protein gene (SEQ ID NO: 5), or the corresponding site in a transcript of the capsid protein gene, or a replication intermediate of the virus,wherein the first and second ribozymes are part of the polyribozyme which has endoribonuclease activity and inactivates the capsid protein gene of the Cucumber Mosaic Virus.