Patent ID: 8426209
Filing Date: 2013-04-23
Classification: B01L,G01N,Y10T

Abstract:
1. A method for cell sorting in a device comprising a microfluidic network having a sample inlet, one or more inlet channels, a main channel, and at least two outlet channels, comprising the steps of: receiving cells in a fluidic medium at the sample inlet, flowing the cells through the main channel, the main channel being divided into one side and another side as defined by the path of the flow of cells, subjecting the cells to a bias flow preferentially resulting in collection of the cells in a first reservoir in fluid communication with a first outlet channel, wherein the bias flow is created by applying asymmetric pressure to one or more of the channels of the microfluidic network, identifying a cell to be sorted through application of a lateral force into a second reservoir coupled to a second outlet channel, and applying a lateral force on the cell, the lateral force consisting of a single force applied from one side of the main channel and not from the said another side of the main channel, and characterized in that the lateral force is a non-trapping force, whereby the cell is moved transverse to the preferentially biased flow and selectively exits the second outlet channel coupled to the second reservoir.