Patent ID: 7655430
Filing Date: 2010-02-02
Classification: C12Q

Abstract:
1. A method for detecting a residual agricultural chemical, comprising reacting reduced glutathione with the residual agricultural chemical, in the presence of a glutathione transferase, to form a choline esterase inhibitor, reacting the choline esterase inhibitor with a choline esterase to alter the activity of the choline esterase, and detecting the presence of the residual agricultural chemical based on changes in the choline esterase activity; wherein the residual agricultural chemical is selected from the group consisting of carbosulfan, furathiocarb, benfuracarb, thiodicarb and alanicarb; wherein the choline esterase inhibitor is selected from the group consisting of carbofuran and methomyl; wherein the reacting of the residual agricultural chemical with the reduced glutathione is conducted in the presence of a chelating agent, at a temperature ranging from 10° C. to 40° C., at a pH ranging from 5.0 to 8.5, and for a time period ranging from 5 to 60 minutes; and wherein the chelating agent is selected from the group consisting of EDTA and citric acid.