Patent ID: 6262264
Filing Date: 2001-07-17
Classification: C07F,C07K,G01N,Y10S,Y10T

Abstract:
An electrochemically detectable compound of the formula ##STR8##whereinR and R.sub.1 are the same or different and are 2,2'-bipyridyl, 4,4'-disubstituted-2,2'-bipyridyl, 5-5'-disubstituted,-2,2'-bipyridyl, 1,10-phenanthrolinyl, 4,7-disubstituted-1,10-phenanthrolinyl, or 5,6-disubstituted-1,10-phenanthrolinyl, wherein each substituent is a methyl, ethyl, or phenyl group,R and R.sub.1 are coordinated to Os through their nitrogen atoms;q is 1 or 0;R.sub.7 is B--(L).sub.k --Q(CH.sub.2).sub.i --;R.sub.2 is hydrogen, methyl, or ethyl when q is 1, and R.sub.2 is B--(L).sub.k --Q(CH.sub.2).sub.i -- when q is 0;wherein in the group B--(L).sub.k --Q(CH.sub.2).sub.i --Q is O, S, or NR.sub.4 wherein R.sub.4 is hydrogen, methyl or ethyl;--L-- is a divalent linker;k is 1 or 0;i is 1, 2, 3, 4, 5 or 6; andB is hydrogen or a group comprising a ligand capable of binding to a specific binding partner;Z is chloro or bromo;m is +1 or +2;X is chloride, bromide, iodide, fluoride, tetrafluoroborate, perchlorate, nitrate, sulfate, carbonate, or sulfite;Y is chloride, bromide, iodide, fluoride, tetrafluoroborate, perchlorate or nitrate; andn is 1 or zero,provided that when X is sulfate, carbonate, or sulfite, n is zero,and when m is 1, n is zero and X is not sulfate, carbonate or sulfite.