Patent ID: 6265160
Filing Date: 2001-07-24
Classification: A61K,C07K,C12Q,G01N

Abstract:
A method of identifying an agent as a candidate Jak-Stat pathway inhibitor by the steps of:(a) obtaining a control lysate prepared from HTLV-1 transformed cells, said control lysate containing IL-2 receptor components including Stat3, Stat5, IL-2R.beta. and .gamma.c;(b) obtaining a test lysate prepared from HTLV-I transformed cells propagated in the presence of said agent, said test lysate containing IL-2 receptor components including Stat3 or Stat5, IL-2R.beta. and .gamma.c;(c) quantitating in each of said lysates an interaction between IL-2R.beta. and Stat3 or Stat5 ; and(d) identifying the agent as an inhibitor of the Jak-Stat pathway if the interaction quantitated in the quantitating step is greater in the control lysate than in the test lysate.