Patent ID: 6875591
Filing Date: 2005-04-05
Classification: C12P

Abstract:
1. A process for producing guanosine 5′-diphosphate-fucose (“GDP-fucose”), comprising: allowing a guanosine 5′-triphosphate (“GTP”) precursor, a saccharide selected from the group consisting of glucose, fructose and mannose, and enzyme sources to be present in an aqueous medium, wherein the enzyme sources are (i) a culture of a microorganism capable of forming GTP from said GTP precursor or a treated product of the culture, and (ii) a culture of a microorganism capable of forming guanosine 5′-diphosphate-4-keto-6-deoxymannose (“GKDM”) from said saccharide and GTP or a treated product of the culture; forming and accumulating GKDM in the aqueous medium; and then adding a culture of a microorganism capable of converting GKDM into GDP-fucose or a treated product of the culture, as an enzyme source, to the aqueous medium to convert the accumulated GKDM into GDP-fucose; forming and accumulating GDP-fucose in the aqueous medium; and recovering the GDP-fucose from the aqueous medium, wherein the treated products of the culture are treated products independently selected from the group consisting of a concentrated product of the culture, a dried product of the culture, cells obtained by centrifuging the culture, a dried product of the cells, a freeze-dried product of the cells, a surfactant-treated product of the cells, a solvent-treated product of the cells, an enzyme-treated product of the cells and an immobilized product of the cells.