Patent ID: 7563600
Filing Date: 2009-07-21
Classification: B01J,B82Y,C12N,C12Q,C40B

Abstract:
1. A composition for the manufacture of a polynucleotide sequence comprising: a first oligonucleotide comprising at its 5′ end a sequence region of about 10 to about 50 bases that is the same as a sequence region at the 3′ end of another oligonucleotide in the composition; a second oligonucleotide comprising at its 3′ end a sequence region of about 10 to about 50 bases that is the same as a sequence region at the 5′ end of another oligonucleotide in the composition; and a plurality of oligonucleotides wherein each oligonucleotide of the plurality comprises a first sequence region of about 10 to about 50 bases at its 5′ end that is the same as a sequence region at the 3′ end of another oligonucleotide in the composition and a second distinct sequence region of about 10 to about 50 bases at its 3′ end that is the same as a sequence region at the 5′ end of a different oligonucleotide in the composition; wherein the oligonucleotides of the composition are attached to a solid or porous surface and wherein the oligonucleotides of the composition together comprise the polynucleotide sequence.