Patent ID: 6783958
Filing Date: 2004-08-31
Classification: C07K,C12N

Abstract:
A nucleic acid molecule encoding an artificial biosensor protein comprising the following sequences (a) to (h) sequentially from the N terminus:(a) Met-Gly-Thr or Met-Val-Asp (linker X); (b) a partial amino acid sequence of myosin light chain kinase protein (Serâ€”Ser-Argâ€”Arg-Lys-Trp-Asn-Lys-Thr-Gly-His-Ala-Val-Arg-Ala-Ile-Gly-Arg-Leu-Serâ€”Ser)(SEQ ID NO: 6); (c) Leu-Glu (linker Y); (d) the amino acid sequence from the 149th to 238th position of green fluorescent protein of SEQ ID NO: 2; (e) Glyâ€”Gly-Thr-Glyâ€”Gly-Ser (linker amino acid sequence; (amino acids 117 to 122 of SEQ ID NO: 8); (f) the amino acid sequence from the 1st to 144th position of green fluorescent protein of SEQ ID NO: 2; (g) Gly-Thr-Arg or Thr-Arg (linker Z); and (h) the amino acid sequence from the 2nd to 148th position of rat calmodulin protein of SEQ ID NO: 4 or the amino acid sequence from the 2nd to the 148th position of calmodulin protein mutant CaMCN, in which residues 82-148 and 9-75 of SEQ ID NO: 4 have been exchanged.