Patent ID: 6399382
Filing Date: 2002-06-04
Classification: C07D,C07K,C12N,C12P

Abstract:
A method to prepare a cell containing at least one nucleic acid molecule, wherein said molecule comprises at least one module that encodes a modular polyketide synthase (PKS) functional in catalyzing the synthesis of a polyketide, said module comprising at least one nucleotide sequence which encodes a PKS acyl transferase (AT) activity; at least one nucleotide sequence which encodes a PKS ketoacyl carrier protein synthase (KS) activity; and at least one nucleotide sequence which encodes a PKS acyl carrier protein (ACP) activity;wherein said module is a 6-deoxyerythronolide B synthase module; said module operatively linked to a control sequence, whereby a functional modular PKS is produced in said cell, with the proviso that said module or said control sequence is heterologous to the host cell; said method comprising introducing said nucleic acid molecule into a host cell.