Patent ID: 6511837
Filing Date: 2003-01-28
Classification: A21D,C11D,C12N

Abstract:
A nucleic acid sequence which comprises:a) a DNA sequence encoding a mature lipolytic enzyme cloned into a plasmid present in Escherichia coli DSM 13537, b) a DNA sequence shown in SEQ ID NO: 1, c) an analogue of the sequence defined in a) or b) which encodes a lipolytic enzyme, wherein the analogue i) has at least 95% homology with said a) or b), or ii) hybridizes with a complementary strand of said a) or b) under hybridization conditions comprising prehybridizing in a solution of 5Ã—SSC, 5Ã—Denhardt's solution, 0.5% SDS and 100 &mgr;g/ml of denatured sonicated salmon sperm DNA, followed by hybridization in the same solution for 12 hours at approx. 45Â° C., followed by washing in 2Ã—SSC, 0.5% SDS for 30 minutes at a temperature of at least 70Â° C., or d) a complementary strand of said a), b) or c).