Patent ID: 8557553
Filing Date: 2013-10-15
Classification: C12N

Abstract:
1. A method for reducing nuclease activity in a subtilisin-comprising fermentation broth solution, the method comprising: a) adjusting the pH of the subtilisin solution to a pH in the range of from pH 8.5 to pH 10.5; b) adding a polyol in an amount of 10% (v/v) to 60% (v/v); c) adjusting the temperature of the subtilisin-comprising fermentation broth solution to a temperature in the range of from 50° C. to 80° C.; and (d) incubating the subtilisin- and nuclease-comprising solution for a period of up to 10 minutes, wherein the nuclease activity is reduced to less than 5% of the initial value and the subtilisin activity is maintained at more than 80% of the initial value.