Patent ID: 6426185
Filing Date: 2002-07-30
Classification: A01H,C07K,C12N,C12Q,Y02A,Y10S

Abstract:
A method of compiling a plant antisense functional gene profile comprising:a) preparing a library of DNA or RNA sequences from a donor plant, and constucting recombinant viral nucleic acids obtained from a tobamo virus comprising an unidentified nucleic acid insert obtained from said library in an antsense orientation relative to said DNA or RNA sequence of said donor plant; b) infecting a plant host with one or more said recombinant viral nucleic acids; c) transiently expressing said unidentified nucleic acid in the plant host; d) determining one or more phenotypic or biochemical changes in the plant host, if any; e) identifying an associated trait where a phenotypic or biochemical change occurs; f) identifying said recombinant viral nucleic acid that results in said one or more changes in the plant host; g) identifying a plant host gene, if any, associated with the trait, and h) repeating steps b)-g) until at least one nucleic acid is associated with said trait, whereby an antisense functional gene profile of the plant host or of the donor plant is compiled.