Patent ID: 9044420
Filing Date: 2015-06-02
Classification: A61K,A61P,C12N

Abstract:
1. A method for priming and boosting a CD8 T cell immune response in a subject, the method comprising (a) administering to the subject at least one dose of a first immunogenic composition comprising (i) a lentiviral vector comprising a nucleotide sequence that encodes a first immunogen or an immunogenic fragment thereof, wherein the lentiviral vector is incorporated into a vector particle, wherein the vector particle comprises a lentiviral vector genome and an alpha virus envelope; and (b) subsequently administering to the subject at least one dose of a second immunogenic composition comprising (i) at least the first immunogen or an immunogenic fragment thereof and (ii) a TRL4 agonist, thereby priming and boosting a CD8 T cell immune response specific for the first immunogen, wherein the TRL4 agonist is a compound of the following structure: wherein A1 and A2 are independently selected from the group of hydrogen, phosphate, and phosphate salts and R1, R2, R3, R4, R5, and R6 are independently selected from the group of hydrocarbyl having 3 to 23 carbons, represented by C3-C23.