Patent ID: 6383755
Filing Date: 2002-05-07
Classification: C12Q

Abstract:
A method of evaluating the fidelity of a given set of polymerase chain reaction conditions, said method comprising:(a) amplifying a template polydeoxyribonucleotide under said polymerase chain reaction conditions using flanking primers to produce a population of amplified product polydeoxyribonucleotides, wherein said template polydeoxyribonucleotide comprises at least one pseudo restriction endonuclease site; (b) contacting said population of amplified product polydeoxyribonucleotides with at least one restriction endonuclease corresponding to said at least one pseudo restriction endonuclease site to produce a cleaved product population; (c) detecting the presence of cleavage products in said cleaved product population; and (d) relating the presence of said cleavage products to the fidelity of said polymerase chain reaction conditions; whereby the fidelity of said polymerase chain reaction conditions is evaluated.