Patent ID: 8481461
Filing Date: 2013-07-09
Classification: C12Q

Abstract:
1. A method of detecting hybridization between a probe polynucleotide and a target polynucleotide with a microarray comprising the steps of a) bringing a fluorescent labeled target polynucleotide into contact with a microarray having a plurality of detection spots in each of which one of a plurality of types of probe polynucleotides is immobilized and at least one reference spot in which at least two types of the probe polynucleotides immobilized in the detection spots are immobilized, and allowing the target polynucleotide to hybridize, b) removing any unhybridized target polynucleotide by washing the microarray, c) measuring fluorescence of the reference spot and determining that if a predetermined value is satisfied the hybridization is reliable, and d) measuring fluorescence of each of the detection spots if it is determined that hybridization is reliable.