Patent ID: 7122330
Filing Date: 2006-10-17
Classification: C07K,C12N,C12Y,G01N

Abstract:
1. A method of producing a plurality of proteins having an activity or property of interest encoded by a library of DNA vectors, wherein the library of vectors comprises a plurality of different vectors, each different vector comprising a different protein-encoding nucleic acid sequence, said nucleic acid sequence being operably linked to an expression-regulating region and optionally a secretion signal encoding sequence, the method comprising the steps of: (a) stably transforming a plurality of individual filamnentous fungi, wherein the fungi are selected from the group consisting of (b) culturing the transformed mutant filamentous fungi under conditions conducive to formation of transferable reproductive elements which are monoclonal and readily dispersed in suspension; (c) separating from one another a plurality of transferable reproductive elements in suspension; (d) transferring the separated transferable reproductive elements to secondary cultures; and (e) culturing into monoclonal cultures or monoclonal colonies the individual transferable reproductive elements in said secondary cultures, under conditions condacive to expression of the proteins encoded by the protein-encoding nucleic acid sequences.