Patent ID: 9128082
Filing Date: 2015-09-08
Classification: G01N

Abstract:
1. A method for detecting a target in a sample comprising pre-incubating a sample with a first binding entity and a third binding entity to form a pre-loading mixture, wherein the first binding entity specifically binds to a target entity on the surface of the target and the third binding entity to form a first binding complex, and wherein the first binding entity is not conjugated to a detectable or capturable entity, passing the pre-loading mixture through a micro-channel, wherein the surface of the micro-channel is coated with a second binding entity capable of specifically binding to the third binding entity, to form a complex of the first binding complex and the second binding entity on the surface of the micro-channel via direct or indirect binding of the third binding entity and the second binding entity, wherein the second binding entity is not an antibody, and detecting the presence of the target on the surface of the micro-channel.