Patent ID: 8551698
Filing Date: 2013-10-08
Classification: B01J,B01L,C07H,C12Q,C40B,Y10S,Y10T

Abstract:
1. A method comprising: loading a sample into a fluidic device comprising a plurality of sample chambers, said sample chambers having respective volumes ranging from about 1 picoliter to about 1 microliter; in the fluidic device, subdividing the sample into a plurality of sample portions such that respective sample portions are positioned in the respective sample chambers, such that at least one of said plurality of sample chambers contains a sample portion having a single molecule of a target nucleic acid wherein a detectable concentration of said target nucleic acid will be attained in said at least one sample chamber after a single round of amplification; and simultaneously subjecting the sample portions loaded into said respective sample chambers to amplification conditions using at least one amplification targeting reagent positioned in said sample chambers.