Patent ID: 6319682
Filing Date: 2001-11-20
Classification: A61K,C12Q,G01N

Abstract:
A method for screening a candidate compound for activity as a chloride cotransporter agonist or antagonist, comprising:introducing an agent to a cell sample that is capable of changing at least one of the ionic concentration and the ionic balance in a cell sample without killing cells;observing and recording at least one property of the cell sample that is representative of at least one of the ionic concentration and the ionic balance in the cell sample following introduction of the agent;introducing a candidate compound to the cell sample;observing and recording at least one property of the cell sample that is representative of at least one of the ionic concentration and the ionic balance in the cell sample following introduction of the candidate compound; andcomparing the at least one property of the cell sample recorded following introduction of the agent with the at least one property of the cell sample recorded following introduction of the candidate compound, and thereby determining changes in at least one of the ionic concentration and the ionic balance in the cell sample.