Patent ID: 8859215
Filing Date: 2014-10-14
Classification: G01N

Abstract:
1. A method for determining the amount of binding of a cell interacting component to a cell surface structure present in a cell sample, comprising: a) pre-labelling said cell interacting component with a first dye; b) incubating said component with desired cells to allow said component to bind to cell surface structures; c) washing away labelled cell interacting component not bound to the cells; d) lysing the cells to form a cell lysate; e) electrophoretic separation of proteins in said cell lysate in a gel, preferably a SDS PAGE gel; f) transferring proteins from the gel to a Western blotting membrane; g) Western blotting detection of housekeeping protein; h) scanning or imaging of the membrane; and i) measuring signals from the pre-labelled cell interacting component and housekeeping protein.