Patent ID: 6194637
Filing Date: 2001-02-27
Classification: C12N

Abstract:
An isolated DNA Ligase I polynucleotide comprising a member selected from the group consisting of:a) a polynucleotide having at least 90% sequence identity to the polynucleotide of SEQ ID NO:1; wherein the percent sequence identity is based on the entire region coding for SEQ ID NO:2 and is determined according to the GAP program in the Wisconsin Genetics Software Package, using the default settings;b) a polynucleotide encoding the polypeptide of SEQ ID NO:2;c) a polynucleotide amplified from a Zea mays nucleic acid library using primers which selectively hybridize, under stringent hybridization conditions, to loci within the polynucleotide of SEQ ID NO:1;d) a polynucleotide which selectively hybridizes, under stringent hybridization conditions and a wash in 0.1.times.SSC at 60.degree. C., to the polynucleotide of SEQ ID NO:1;e) the polynucleotide of SEQ ID NO: 1;f) a polynucleotide which is complementary to a polynucleotide of (a), (b), (c), (d), or (e); andg) a polynucleotide comprising at least 50 contiguous nucleotides from a polynucleotide of (a), (b), (c), (d), (e), or (f); wherein the polynucleotide of (a), (c), (d), or (g) each encode a maize DNA Ligase I polypeptide.