Patent ID: 9144546
Filing Date: 2015-09-29
Classification: A61K,C12N

Abstract:
1. A method of making a composition comprising at least about 0.5 mg/ml of a nucleic acid suspended in an aqueous solution, the method comprising: combining a nucleic acid, a cationic lipopolymer, and a filler excipient in an aqueous medium, the cationic lipopolymer consisting of polyethyleneimine (PEI) covalently linked independently to cholesterol and/or polyethylene glycol (PEG) groups, and where the average PEG:PEI:cholesterol molar ratio in the cationic lipopolymer is within the range of 1-5 PEG:1 PEI:0.4-1.5 cholesterol; lyophilizing the mixture to a powder; and reconstituting the powder with a diluent to form a solution comprising at least about 0.5 mg/ml nucleic acid complexed with the cationic lipopolymer.