Patent ID: 8809020
Filing Date: 2014-08-19
Classification: C12Q,Y10T

Abstract:
1. A method of detecting extracellular RNA in blood plasma or serum from a human with breast cancer, wherein said extracellular RNA encodes an epidermal growth factor receptor (EGFr) or HER2/neu which is a target of a monoclonal antibody therapy, the method comprising the steps of: a) extracting extracellular RNA from blood plasma or serum of a human with breast cancer; b) amplifying in a qualitative or quantitative fashion a portion of the RNA extracted in step a) or cDNA derived therefrom using primers or probes specific to a RNA that encodes an epidermal growth factor receptor (EGFr) or HER2/neu which is a target of a monoclonal antibody therapy or its cDNA, and producing an amplified product; and c) detecting the amplified product, [whereby in a human with breast cancer] wherein the presence of the amplified product indicates that the RNA that encodes an epidermal growth factor receptor (EGFr) or HER2/neu which is a target of a monoclonal antibody therapy is detected in said human's blood plasma or serum.