Patent ID: 6103084
Filing Date: 2000-08-15
Classification: C12M

Abstract:
An apparatus for electroporation for delivering a predetermined charge to a suspension of cells comprising DNA and cloned DNA for combining the cloned DNA with the cells comprising DNA, the apparatus comprising:a. means for generating pulses of a controlled amount of energy;b. means for coupling the pulses to a capacitor, the means for coupling coupled to the means for generating and to the capacitor;c. means for counting the number of pulses necessary to store a predetermined charge on the capacitor, the means for counting coupled to the means for generating and to the means for coupling for counting the number of pulses coupled to the capacitor;d. means for measuring a charge stored on the capacitor when a threshold value is reached, the means for measuring coupled to the capacitor;e. means for calculating a charge per each of the pulses value and a remaining number of pulses required to completely store the predetermined charge on the capacitor, the means for calculating coupled to the means for counting and to the means for measuring;f. means for controlling coupled to the means for calculating, to the means for generating and to the means for coupling, for controlling the generation and coupling of the remaining number of pulses to the capacitor; andg. means for transferring energy stored on the capacitor to a cuvette after the remaining number of pulses have been coupled to the capacitor, wherein the means for transferring is coupled to the capacitor, to the load and to the means for controlling, and further wherein the means for controlling also controls the means for transferring.