Patent ID: 7838292
Filing Date: 2010-11-23
Classification: A61K,A61P,C12N

Abstract:
1. A method for preparing cultured cells from human olfactory neuroepithelium, comprising: dispersing adult human tissue comprising olfactory neuroepithelium to form a cell population comprising separated cells; culturing said cell population in a culture medium as an adherent cell population to induce formation of a suspended cell population, wherein said suspended cell population comprises neurospheres, wherein said suspended cell population comprises one or more cells that are each immunoreactive for both nestin and beta-tubulin isotype III; and subculturing said suspended cell population, wherein said subculturing comprises collecting said suspended cell population comprising neurospheres; dispersing said neurospheres into separated cells; and culturing said separated cells, wherein said suspended cell population doubles every 18-24 hours in Olfactory Epithelial Medium (OEM) at 37° C. in an atmosphere of humidified 5% CO detecting the presence of cells in the suspended cell population that are each immunoreactive for both nestin and beta-tubulin isotype III with antibodies or antigen-binding fragments thereof that bind nestin and beta tubulin isotype III.