Patent ID: 8168774
Filing Date: 2012-05-01
Classification: A01K,A61K,A61P,C12N

Abstract:
1. A double-stranded DNA construct comprising two copies of a structural gene region whose nucleotide sequence is identical to the nucleotide sequence of a region of a target gene in an animal cell, wherein one of the two copies is in the sense orientation and the other of the two copies is in the antisense orientation operably under the control of a single promoter sequence which is operable in the cell, and wherein the copy of the structural gene region in the sense orientation and the copy of the structural gene region in the antisense orientation are arranged as an interrupted palindrome sequence such that the copy of the structural gene region in the sense orientation and the copy of the structural gene region in the antisense orientation are separated and linked by nucleotides other than the nucleotides of the copy of the structural gene region in the sense orientation and the copy of the structural gene region in the antisense orientation.