Patent ID: 7829084
Filing Date: 2010-11-09
Classification: A61K,A61P,C07K

Abstract:
1. A non-naturally occurring single chain Fv protein comprising: i) a first polypeptide having a G28-1 single chain Fv binding domain polypeptide, said binding domain polypeptide comprising a light chain variable region and a heavy chain variable region, wherein a serine is at position 11 in the first framework region of the heavy chain variable region; ii) a second polypeptide comprising an altered wild type IgG1 immunoglobulin hinge region attached to said first polypeptide, wherein the wild type IgG1 hinge region comprises first, second and third cysteine residues and a proline, wherein the first cysteine is N-terminal to the second cysteine, the second cysteine is N-terminal to the third cysteine, and the third cysteine is N-terminal to the proline, and wherein in the altered hinge region said first and second cysteine residues are substituted with serine and said proline residue is substituted with serine; and iii) a third polypeptide comprising an N-terminally truncated immunoglobulin heavy chain constant region polypeptide attached to the second polypeptide, wherein said heavy chain constant region comprises CH2 and CH3 domains from IgG