Patent ID: 7078179
Filing Date: 2006-07-18
Classification: C07K,C12N,G01N

Abstract:
1. A method for identifying host cells comprising a heterologous polynucleotide sequence, said method comprising: introducing to a population of cells a polynucleotide construct, said polynucleotide construct comprising a heterologous polynucleotide sequence the presence of which is desired in said host cells, and a marker polynucleotide sequence which encodes an alpha-(1,3)-galactosyl (αGal) epitope conferring protein, said marker polynucleotide sequence operably linked to a promoter sequence within said polynucleotide construct operable in said host cells, wherein said αGal epitope conferring protein is N-acetyllactosaminide 3-apha galactosyltransferase (αGT), and wherein said population of cells in αGT negative.