Patent ID: 7970553
Filing Date: 2011-06-28
Classification: C12Q,G16B

Abstract:
1. A method of selecting probes for analysis of a set of designated nucleic acid sequences by means of a sequence of a first step of selective conversion of cognate nucleic acid subsequences in the designated set of sequences into a related second set of converted sequences using a set of conversion probes, wherein members of the set of conversion probes include a subsequence which is complementary to the cognate nucleic acid subsequences and a second step of capturing said converted sequences to a set of detection probes, wherein members of the set of detection probes include a subsequence which is complementary to cognate target subsequences in the converted sequences, the method comprising: providing said set of nucleic acid sequences; performing an iterative optimization of selected probes as follows: outputting the set of conversion probes and the set of detection probes satisfying the convergence criteria to a display; and using the set of detection probes satisfying the convergence criteria to perform analysis of the set of nucleic acid sequences in an assay wherein members of the set of detection probes hybridize with members of the set of nucleic acid sequences, which is detected.