Patent ID: 6753141
Filing Date: 2004-06-22
Classification: C12Q

Abstract:
A method of identifying a sequence alteration in a target nucleic acid as compared to a control nucleic acid, said method comprising:a) hybridizing a plurality of nucleic acid probes with said target nucleic acid, wherein said probes are complementary to different overlapping regions of said control nucleic acid; b) determining the melting temperature (Tm) of at least two overlapping probes from said target nucleic acid; c) determining the &Dgr;Tm for each of said overlapping probes, wherein the &Dgr;Tm is the difference between the Tm of said target nucleic acid and one of said overlapping probes and the Tm of said control nucleic acid and the same overlapping probe; and d) determining the &Dgr;&Dgr;Tm, wherein the &Dgr;&Dgr;Tm is the difference, if any, in the &Dgr;Tm of at least two overlapping probes, as an indication of the presence or absence of a sequence alteration in said target nucleic acid as compared to said control nucleic acid.