Patent ID: 6673575
Filing Date: 2004-01-06
Classification: A61K,C07K,C12N,C12P

Abstract:
A process for increasing the glycosylation of a polypeptide when producing glycosylated polypeptides in mammalian or insect cells while maintaining cell specific productivity comprising:(a) culturing mammalian or insect cells in a culture medium; (b) monitoring the concentration of at least one parameter selected from nutrients and metabolites of the cultured cells wherein said parameter correlates to the nutrient requirement of the cells and determining the consumption rate of said parameter; (c) adding a mixture of at least two carbohydrates to the culture medium in an amount sufficient to meet the nutrient requirements of the cells to maintain cell specific productivity, wherein said amount is based on the consumption rate of the parameter in step (b); and (d) isolating the glycosylated polypeptide from the culture medium, wherein culturing the cells under the condition (a)-(c) increases the glycosylation of the polypeptide while maintaining cell specific productivity.