Patent ID: 8859203
Filing Date: 2014-10-14
Classification: C12Q

Abstract:
1. A method for determining the presence or absence of HBV genotype A in a biological sample, comprising: (i) providing a biological sample comprising polynucleic acids; (ii) optionally releasing, isolating and/or concentrating the polynucleic acids present in the sample; (iii) optionally amplifying the HBsAg region, or part thereof, of the HBV gene present in said sample with at least one primer pair; (iv) hybridizing the polynucleic acids of step (i) or (ii) or (iii) with at least one nucleotide probe selected from the group consisting of SEQ ID NO: 77, SEQ ID NO: 140, SEQ ID NO: 193, the complement of SEQ ID NO:77, the complement of SEQ ID NO:140, and the complement of SEQ ID NO:193; (v) detecting the hybrid(s) formed in step (iv); (vi) inferring the presence of absence of HBV genotype A in said sample from the hybridization signal(s) obtained in step (v).