Patent ID: 9150910
Filing Date: 2015-10-06
Classification: C12Q

Abstract:
1. A method of generating a complex comprising a nucleic acid target molecule, said method comprising: combining in a reaction mixture wherein said combining is under reaction conditions sufficient to provide for specific binding of said nucleic acid target molecule, if present, to said target-immobilization probe, and wherein said combining is under reaction conditions sufficient to provide for specific binding of said linking molecule to said nucleic acid target molecule, if present, and to a plurality of said capture probes, wherein, when said nucleic acid target molecule is present in said sample, said target-immobilization probe specifically binds to said nucleic acid target molecule, said nucleic acid target molecule specifically binds to said linking molecule, and said linking molecule specifically binds to a plurality of the capture probes bound to the detectable particle, thereby forming a complex comprising said substrate surface, said target immobilization probe, said nucleic acid target molecule, said linking molecule and said detectable particle, and wherein said complex does not comprise more than one of said detectable particle.