Patent ID: 6368803
Filing Date: 2002-04-09
Classification: C12Q

Abstract:
A kit for detection of a polynucleotide comprising in packaged combination:(a) a first oligonucleotide having the characteristic that, when reversibly hybridized under isothermal conditions to at least a portion of said polynucleotide, it is degraded by a 5â€²-nuclease to provide (i) a first fragment that is substantially non-hybridizable to said polynucleotide and (ii) a second fragment that is 3â€² of said first fragment in said first oligonucleotide and is substantially hybridizable to said polynucleotide, wherein said is isothermal conditions are at or near the melting temperature of a duplex comprising the oligonucleotide hybridized to the polynucleotide, (b) a second oligonucleotide having the characteristic of hybridizing to a site on said polynucleotide that is separated by no more than one nucleotide from the 3â€²-end of the site at which said first oligonucleotide wherein said polynucleotide is substantially fully hybridized to said second oligonucleotide under said isothermal conditions, and (c) a 5â€²-nuclease.