Patent ID: 8535881
Filing Date: 2013-09-17
Classification: C12Q

Abstract:
1. A method for high speed parallel molecular nucleic acid sequencing, comprising: (a) attaching a nucleic acid molecule to be sequenced to a substrate; (b) adding an oligonucleotide primer under conditions where the primer anneals to the nucleic acid molecule; (c) adding a green fluorescent protein (GFP)-polymerase and nucleotides, wherein the nucleotides are labeled with a fluorophore, and wherein each type of nucleotide comprises a different fluorophore that emits a unique emission signal corresponding to addition of that type of nucleotide as the nucleotide is incorporated into a complementary nucleic acid molecule by the GFP-polymerase; (d) adding four deoxyribonucleoside monophosphates (dNMPs) or deoxyribonucleoside diphosphates (dNDPs), wherein the dNMPs or dNDPs comprise a fluorophore distinct from the fluorophores on the nucleotides; and (e) detecting the unique emission signal.