Patent ID: 7217543
Filing Date: 2007-05-15
Classification: C12N,C12P

Abstract:
1. A method for producing an L-amino acid comprising: a) culturing a microorganism having the ability to produce said L-amino acid in a medium, whereby said L-amino acid accumulates in the medium, and b) collecting said L-amino acid from the medium, wherein said microorganism is a methanol-utilizing bacterium having the Entner-Doudoroff pathway and is modified so that 6-phosphogluconate dehydratase activity and/or 2-keto-3-deoxy-6-phosphogluconate aldolase activity are/is enhanced as compared to a wild-type bacterium, and said L-amino acid is selected from L-amino acids produced by a biosynthetic pathway which utilizes pyruvic acid as an intermediate, wherein said 6-phosphogluconate dehydratase activity is enhanced by A) increasing the copy number of a gene coding for 6-phosphogluconate dehydratase as compared to a wild-type bacterium, or B) replacing the native promoter of said gene with a stronger promoter so that expression of the gene is enhanced in said bacterium as compared to a wild-type bacterium, and wherein said 2-keto-3-deoxy-6-phosphogluconate aldolase activity is enhanced by C) increasing the copy number of a gene coding for 2-keto-3-deoxy-6-phosphogluconate aldolase as compared to a wild-type bacterium, or D) replacing the native promoter of said gene with a stronger promoter so that expression of the gene is enhanced in said bacterium as compared to a wild-type bacterium.