Patent ID: 6376206
Filing Date: 2002-04-23
Classification: C07K,G01N,Y10S

Abstract:
A method for the immunoassay determination of myocardial necroses in a patient, wherein detecting muscle injury can be accomplished for at least 150 hours after occurrence of an infarction, said method comprising:a) incubating a body fluid sample of the patient with i) at least one antibody to human cardiac muscle troponin T having cross-reactivity to human skeletal muscle troponin T which is less than 5% as determined by ELISA and cross-reactivity to troponin I and other myofibrillar proteins of less than 2% as determined by ELISA, and ii) a binding partner B for either human cardiac muscle troponin T or the antibody, wherein either the antibody or the binding partner B is labeled with a determinable group, to form an immunological complex containing the determinable group; b) isolating the immunological complex from the remainder of sample; and c) determining the determinable group either in the isolated complex or in the sample remaining as an indicator of the human cardiac muscle troponin T in the sample; thereby determining the occurrence of injury to a cardiac muscle of the patient.