Patent ID: 6406854
Filing Date: 2002-06-18
Classification: C12Q

Abstract:
A method for estimating the location of the crossover point of a polymer separation system, the method comprising:(a) electrophoresing a plurality of polynucleotide pairs through a polymer separation system, wherein each polynucleotide pair comprises a first polynucleotide and a second polynucleotide; and (b) detecting a signal associated with the first polynucleotide and a signal associated with the second polynucleotide in each polynucleotide pair; (c) identifying a first polynucleotide pair in which the signal associated with the first polynucleotide of said first polynucleotide pair is not resolved from the signal associated with the second polynucleotide of said first polynucleotide pair; (d) identifying a second polynucleotide pair in which the signal associated with the first polynucleotide of said second polynucleotide pair is resolved from the signal associated with the second polynucleotide of said second polynucleotide pair, and (e) identifying a region in the polymer separation system between the first polynucleotide pair and the second polynucleotide pair, thereby estimating the location of said crossover point in said polymer separation system.