Patent ID: 7799904
Filing Date: 2010-09-21
Classification: C12N

Abstract:
1. An isolated nucleic acid molecule comprising a polynucleotide sequence selected from the group consisting of: a) a gilOI polynucleotide sequence that remains hybridized to the entirety of the full length complement of bases 19892 to 21391 of SEQ ID NO: 1 under stringent conditions of washing twice with 2 molar SSC buffer at a temperature of 45° C. and then twice with 0.1 molar SSC buffer at a temperature of 68° C., and encodes a polypeptide having FAD-dependent oxygenase activity; b) a gilOII polynucleotide sequence that remains hybridized to the entirety of the full length complement of bases 11513 to 12196 of SEQ ID NO: 1 under stringent conditions of washing twice with 2 molar SSC buffer at a temperature of 45° C. and then twice with 0.1 molar SSC buffer at a temperature of 68° C., and encodes a post-poly-ketide synthase (post-PKS) tailoring polypeptide having oxygenase activity; c) a gilOIII polynucleotide sequence that remains hybridized to the entirety of the full length complement of bases 6576 to 7769 of SEQ ID NO: 1 under stringent conditions of washing twice with 2 molar SSC buffer at a temperature of 45° C. and then twice with 0.1 molar SSC buffer at a temperature of 68° C., and encodes a post-PKS tailoring polypeptide having oxygenase activity; and d) a gilOIV polynucleotide sequence that remains hybridized to the entirety of the full length complement of bases 26200 to 27552 of SEQ ID NO: 1 under stringent conditions of washing twice with 2 molar SSC buffer at a temperature of 45° C. and then twice with 0.1 molar SSC buffer at a temperature of 68° C., and encodes a polypeptide having FAD-dependent oxygenase activity.