Patent ID: 7850957
Filing Date: 2010-12-14
Classification: A61K,A61P,C12N

Abstract:
1. A genic expression adenoviral hybrid vector for expressing a gene of interest directly in an adenovirus-permissive cell in which a tissue-specific promoter is active, wherein said tissue specific promoter is the tumor specific promoter AFP, characterized in that the genic expression adenoviral hybrid vector comprises at least the following elements, oriented in the direction 5′ to 3′: i. a first chain of adenoviral origin comprising a first inverted terminal repeat (ITR) sequence and a signal sequence for packaging of the adenovirus; ii. a first non-encoding stuffer sequence; iii. a sequence corresponding to a tissue specific promoter, wherein said tissue specific promoter is the tumor specific promoter AFP; iv. a chain of cDNA derived from an alphavirus, the sequence of which is partly complementary to an alphaviral RNA sequence, comprising at least a sequence encoding for at least one exogenous gene of interest; said chain of cDNA derived from an alphavirus comprising: v. a polyadenylation sequence; and vi. a second adenoviral inverted terminal repeat (ITR) sequence.