Patent ID: 8440434
Filing Date: 2013-05-14
Classification: C12N,C12P

Abstract:
1. A method for producing monatin or salt thereof comprising: contacting HEXAspC aminotransferase (SEQ ID NO:76) or YfdZ (SEQ ID NO:92) with tryptophan to facilitate a conversion of tryptophan to indole-3-pyruvate; contacting 4-hydroxy-2-oxoglutarate aldolase (KHG) (EC 4.1.3.16) or 4-hydroxy-4-methyl-2-oxoglutarate aldolase (ProA) (EC 4.1.3.17) with indole-3-pyruvate to facilitate a conversion of indole-3-pyruvate to 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid; and contacting HEXAspC aminotransferase (SEQ ID NO:76) or YfdZ (SEQ ID NO:92) with 2-hydroxy-2-(indole-3-ylmethyl)-4-keto-glutaric acid to facilitate a conversion of 2-hydroxy-2-(indole-3-ylmethyl)-4-keto-glutaric acid to monatin or salt thereof.