Patent ID: 7829845
Filing Date: 2010-11-09
Classification: G01N,H01J

Abstract:
1. A method for identification of glycosylated proteins and peptides, comprising the steps of: ionizing a sample to form negative ions; carrying out tandem mass spectrometry of the negative ions through collision-induced dissociation (CID) or infrared multi-photon dissociation (IRMPD) to yield CID or IRMPD mass spectrometric data; extracting corresponding glycan information from the CID or IRMPD mass spectrometric data; ionizing the sample to form positive ions; carrying out tandem mass spectrometry of the positive ions through electron capture dissociation (ECD) or electron transfer dissociation (ETD) to yield ECD or ETD mass spectrometric data; extracting corresponding information on a protein or peptide and information on a glycosylation site from the ECD or ETD mass spectrometric data; and carrying out identification of the sample based on the extracted glycan information, and the extracted information on the protein or peptide and the extracted information on the glycosylation site.