Patent ID: 8206702
Filing Date: 2012-06-26
Classification: A61K,A61P,C12N

Abstract:
1. A method for the expansion of tumour-reactive CD4+ T helper and/or CD8+ T-lymphocytes, the method comprising: i) a first phase of stimulating tumour-reactive CD4+ T helper or CD8+ T-lymphocytes with a tumour-derived antigen together with at least one substance having agonistic activity towards an IL-2 receptor to promote survival of tumour-reactive CD4+ T helper or CD8+ T-lymphocytes; and ii) a second phase of activating and promoting growth of tumour-reactive CD4+ T helper or CD8+ T-lymphocytes, wherein the second phase is initiated when the CD25 cell surface marker is down-regulated on CD4+ T helper or CD8+ T-lymphocytes, wherein down-regulation is defined as that 5% or less of the T-lymphocyte population expresses CD25 and wherein the second phase is initiated by the addition of tumour-derived antigen to the T-lymphocytes for activating tumour-reactive CD25-negative T-lymphocytes.