Patent ID: 6436666
Filing Date: 2002-08-20
Classification: C07K,C12N,C12Q,G01N

Abstract:
A polypeptide consisting of a polyamino acid sequence, said polypeptide being useful for screening test compounds for protease regulating activity, said polyamino acid sequence comprising an anchor region, a protease recognition site, and a detectable signal region, wherein the anchor region is bound to a protein-coated solid support by means of a protein-protein interaction, and the protease recognition site comprises a) a cleavable peptide bond, and b) at least 3 naturally adjacent amino acids on each side of the cleavable peptide bond, and wherein the protease recognition site is an HCV protease recognition site selected from the group consisting of 4A/4B, 4B/5A, 5A/5B, and combinations thereof.