Patent ID: 8597935
Filing Date: 2013-12-03
Classification: G01N

Abstract:
1. A particle-sorting method using acridine orange to sort cells in blood, comprising the steps of: staining cells in the blood with acridine orange; irradiating light on the cells in the blood; receiving the irradiated light with detectors; measuring the sizes of red blood cells and platelets by detecting forward scatter (FS) using a detector for small-angled forward-scattered light; measuring the amount of granules by detecting side scatter (SS) using a detector for lateral-scattered light; measuring the amount of DNA by detecting a first fluorescence (FL measuring the amount of RNA by detecting a second fluorescence (FL determining the intracellular DNA concentration and intracellular RNA concentration by dividing the amount of DNA (FL constructing a scattergram of DNA concentration and RNA concentration; and sorting the cells in the blood using the scattergram.