Patent ID: 7962289
Filing Date: 2011-06-14
Classification: G16B

Abstract:
1. A method for analyzing data generated by probe arrays to identify alternative splicing events, comprising: (a) providing a computer with a computer program, wherein said program is designed to receive a first selection of at least two data files and a second selection identifying one or more subsets of intensity values within each data file, and wherein each subset comprises one or more of the intensity values acquired from a biological probe array; (b) opening one of the data files using the computer program; (c) identifying the intensity values associated with one or more of the selected subsets; (d) determining one or more parameters for processing by the computer program; (e) normalizing, with the computer program, the identified intensity values of the one or more selected subsets using the one or more parameters, wherein the normalization is based upon a normalization subset of intensity values, wherein the normalization subset comprises one or more intensity values of the one or more selected subsets, wherein intensity values of the normalization subset are selected without analyzing every value of the one or more selected subsets; (f) determining, using the computer program, a signal estimate for each identified intensity value; (g) storing the parameters and the signal estimates for the identified intensity values; (h) closing the data file; (i) repeating steps (b)-(h) with the computer program for each of the selected data files; and (j) processing each of the stored signal estimates for the identified intensity values using the one or more parameters and the computer program thereby identifying one or more alternative splicing events.