Patent ID: 6174669
Filing Date: 2001-01-16
Classification: C07B,C07H,C12N,C40B

Abstract:
A method for making full-length cDNA libraries, which is for making libraries of cDNAs having lengths corresponding to full lengths of respective mRNAs, and comprises the following steps of:binding a tag molecule to a diol structure present in 5' Cap (.sup.7Me G.sub.ppp N) sites of mRNAs by oxidizing the 5' Cap site diol to form a dialdehyde and reacting the resulting dialdehyde with a tag molecule having a group reactive with the dialdehyde,forming RNA-DNA hybrids by reverse transcription using primers and the mRNAs binding the tag molecule as templates, andseparating RNA-DNA hybrids carrying a DNA corresponding to a full-length of mRNA from the RNA-DNA hybrids formed above by using a function of the tag molecule.