Patent ID: 8729036
Filing Date: 2014-05-20
Classification: A61K,C07H,C12N

Abstract:
1. A method of activating target-specific RNA interference (RNAi) in a mammalian cell in vitro, comprising introducing into said cell an isolated, single-stranded small interfering RNA molecule (ss-siRNA) having a length of 19-21 nucleotides, wherein the sequence of said ss-siRNA molecule is complementary to a target mRNA sequence and directs target-specific RNA interference (RNAi) and wherein the 5′ nucleotide is 5′ phosphorylated or is capable of being 5′ phosphorylated in vitro, said ss-siRNA being introduced in an amount sufficient for degradation of the target mRNA to occur, thereby activating target-specific RNAi in the cell.