Patent ID: 6858404
Filing Date: 2005-02-22
Classification: C07K,C12P

Abstract:
1. A process for the preparation of an epothilone selected from epothilone A or epothilone B, which comprises (a) preparing recombinant host by introducing into a host cell a group of nucleotide fragments that code for epothilone synthase proteins, which group of nucleotide fragments comprises an epoA nucleotide fragment, an epoP nucleotide fragment, an epoB nucleotide fragment, an epoC nucleotide fragment, an epoD nucleotide fragment, an epoE nucleotide fragment, and an epoF nucleotide fragment such that each nucleotide fragment is operatively linked to a promoter sequence; wherein (b) growing the recombinant host under conditions that allow biosynthesis of the epothilone, in the recombinant host; and (c) isolating the epothilone.