Patent ID: 8148095
Filing Date: 2012-04-03
Classification: A61P,C12Q,G01N

Abstract:
1. A method of predicting whether an agent which is a psychotropic drug or a candidate psychotropic drug will be orexigenic, comprising the steps of: (i) contacting the agent in vitro with a histamine H (ii) contacting the agent in vitro with a hypothalamic adenosine monophosphate kinase (AMPK) and determining if the agent increases phosphorylation or increases activity of the AMPK by comparison to a control with no agent present; and (iii) identifying the agent as likely to be orexigenic when it both (a) binds to H1R or inhibits histamine binding to H1R, and (b) increases phosphorylation of hypothalamic AMPK or increases hypothalamic AMPK activity.