Patent ID: 8309354
Filing Date: 2012-11-13
Classification: A61P,C12N

Abstract:
1. A population of human solid cancer stem cells, wherein said cancer stem cells have been isolated from human solid tumor tissue, and grown in a nutrient media optimized for their growth, and wherein said population of human solid cancer stem cells is sufficiently free of other cell types such that an inoculum of about 200 of said cancer stem cells is capable of forming tumors within eight weeks in an immune-deficient mouse, and said cancer stem cells are: a) colorectal carcinoma stem cells that express cell surface marker CD34 and not CD24; b) lung carcinoma stem cells that express cell surface marker CD34 and not CD24. c) pancreatic carcinoma stem cells that express cell surface marker CD34 and not CD24; d) rectal carcinoma stem cells that express CD44 and not CD24; e) Merkel cell carcinoma stem cells that express CD34 and not CD24; f) prostate carcinoma stem cells that express CD34 and not CD24; or g) breast carcinoma stem cells that express CD24 and not CD34.