Patent ID: 7022520
Filing Date: 2006-04-04
Classification: C12N,G01N

Abstract:
1. A composition comprising a cell culture medium and pancreatic islet cells cultured in said cell culture medium in vitro, said culture medium comprising 1–150 mg/L arginine; 1–120 mg/L proline; 1–3050 mg/L nicotinamide; 0.1–100 mg/L transferrin chelated with iron; greater than 10 −11 M insulin or insulin-like growth factors; 10 −12 M-10 3 M glucocorticoid steroid; 1–6000 μg/L zinc salt; 1–250 μg/L manganese salt; 1–1000 μg/L copper salt; 1–150 μg/L selenium salt; 2.0–10.0 mM L-glutamine; 0.01–5.0 g/L D-galactose or 0.01–5.0 g/L D-glucose, or when both D-galactose and D-glucose are included together, 0.01–8.0 g/L.