Patent ID: 6344172
Filing Date: 2002-02-05
Classification: B01D,B03C,G01N

Abstract:
An apparatus for the separation of proteins in a sample, said apparatus comprisingsample solution input means, a first liquid chromatography column, at least one multiport injection valve connecting said sample solution input means to said column, a second liquid chromatography column in communication with said multiport injection valve, said second column being operative successively with or alternatively to said first column, at least one of said first and second columns being packed with a particulate chromatography material comprising perfusive particles which permit convective fluid transport both within and between the particles, or non-porous particles, thereby to confer on said column a chromatographically effective mobile phase transit time therethrough less than five minutes, pump means for providing variable pressure delivery of solutions to said columns via said multiport valve, plural solution reservoirs, a mixing valve, connecting said solution reservoirs to said columns, operative to mix solutions from said reservoirs, and program means for specifying a sequence of system control programs, the mixing of solution by said mixing valve, and the delivery of said mixed solution to said columns via said multiport injection valve.