Patent ID: 8809000
Filing Date: 2014-08-19
Classification: C07K,G01N

Abstract:
1. A method of screening for a compound that putatively blocks or activates sweet taste signaling, the method comprising the steps of: (a) contacting cells comprising isolated mammalian cells or cultured mammalian cells with one or more compounds, wherein said cells express a receptor that binds to sweet taste compounds; and (b) detecting whether said one or more compounds specifically bind to said receptor and/or specifically activate said receptor and, based thereon, identifying said one or more compounds as compounds that putatively block or activate sweet taste signaling, wherein (1) said receptor that binds to sweet compounds comprises a first polypeptide and a second polypeptide; (2) said first polypeptide is selected from the group consisting of: (3) said second polypeptide is selected from the group consisting of: wherein said stringent hybridization conditions comprise conducting the hybridization reaction at 42° C. in a solution comprising 50% formamide, 5×SSC, and 1% SDS and washing at 65° C. in a solution comprising 0.2×SSC and 0.1% SDS.