Patent ID: 6051392
Filing Date: 2000-04-18
Classification: A61B,Y10S

Abstract:
A method for quantitating a substrate comprising the steps of:preparing an analysis element comprising a needle electrode, a membrane electrode and a cylinder having a hollow space, said hollow space being open at least one end thereof, wherein said needle electrode is accommodated in said hollow space and said membrane electrode is formed on an inner wall of said hollow space;pressing an opening of said cylinder against a subject to be examined;projecting a tip of said needle electrode external to said opening of said cylinder temporarily to puncture said subject and collecting a sample from said subject;introducing the collected sample between said membrane electrode and said needle electrode in said cylinder;reacting a substrate contained in said sample with an oxidoreductase; andapplying a voltage across said membrane electrode and said needle electrode and measuring resultant current in said membrane electrode or said needle electrode.