Patent ID: 6224775
Filing Date: 2001-05-01
Classification: G01N

Abstract:
A method for separating a mixture of at least two chemical compounds by capillary electrochromatography which produces a reduced plate height of 3 or less, the steps which comprise:loading a frit material into a capillary, said capillary having first and second portions, said loading occurring in at least said first portion of said capillary;sintering said frit material at said first portion of said capillary to form a first retaining frit;removing substantially all unsintered frit material from said capillary;loading a packing material into said capillary between said first retaining frit and said second portion of said capillary to form a stationary phase, said packing material comprising a silica gel substrate coated with a polysaccharide chiral selector;loading said frit material in said second portion of said capillary adjacent to said stationary phase;sintering said frit material to form a second retaining frit adjacent to said stationary phase;removing substantially all unsintered frit material from said capillary;introducing said mixture into said capillary at one of said retaining frits; andinducing migration of said mixture across said stationary phase by applying a voltage across said capillary, wherein at least one of said chemical compounds is separated from said mixture by exhibiting an electroosmotic flow velocity different from other said chemical compounds in said mixture.