Patent ID: 6608189
Filing Date: 2003-08-19
Classification: C07K,G01N,Y10S

Abstract:
A polynucleotide comprising a nucleotide sequence encoding a functional engineered fluorescent protein whose emission intensity changes as pH varies between 5 and 10, wherein said functional engineered fluorescent protein is selected from the group consisting ofa) a protein comprising the substitutions S65G/S72A/T203Y/H231L within the amino acid sequence of Aequorea green fluorescent protein (SEQ ID NO:2), b) a protein comprising the substitutions S65G/V68L/Q69K/S72A/T203Y/H231L within the amino acid sequence of Aequorea green fluorescent protein (SEQ ID NO:2), c) a protein comprising the substitutions K26R/F64L/S65T/Y66W/N146I/M153T/V163A/N164H/H231L within the amino acid sequence of Aequorea green fluorescent protein (SEQ ID NO:2), d) a protein comprising the substitution H148G within the amino acid sequence of Aequorea green fluorescent protein (SEQ ID NO: 2), and e) a protein comprising the substitutions S65G/V68L/S72A/Q80R/H148Q/T203Y or S65G/S72A/H148Q/T203Y/H231L within the amino acid sequence of Aequorea green fluorescent protein (SEQ ID NO:2), where the amino acid sequence of said functional engineered fluorescent protein is at least 95% homologous to the amino acid sequence of SEQ ID NO:2.