Patent ID: 7270979
Filing Date: 2007-09-18
Classification: C07K

Abstract:
1. A method for producing a glycosylated biologically active alpha 1-antitrypsin polypeptide having the amino acid coding sequence of alpha 1-antitrypsin in the yeast Pichia pastoris in a fermentor, comprising the step of culturing a strain of said yeast selected from the group consisting of KM71 and SMD1168H, which strain comprises an expression cassette that contains a copy of a DNA sequence operably encoding said alpha 1-antitrypsin and operably associated with DNA encoding the yeast Saccharomyces cerevisiae alpha mating factor pre-pro sequence under the regulation of a promoter obtained from a methanol responsive gene of Pichia pastoris in a medium at a pH of about 6.8.