Patent ID: 6429026
Filing Date: 2002-08-06
Classification: G01N,Y10S

Abstract:
A method for performing a non-competitive heterogeneous immunoassay comprising an analyte specific component, an insulating layer, and a fluorescently labelled analyte specific component comprising the steps of(a) immobilizing said analyte specific component 1) directly on a reaction well or 2) on a solid phase attached to a reaction well wherein said analyte specific component is in a single continuous region of said reaction well, (b) adding an insulating layer which separates the analyte specific component from the labelled analyte specific component, which labelled analyte specific component can be antibodies or antigens, before drying, (c) adding said labelled analyte specific component, (d) drying said analyte specific component, said insulating layer, and said labelled analyte specific component in said reaction well, followed by the steps of: (e) adding a sample containing an unknown amount of analyte to be analyzed, (f) allowing said analyte to react with said analyte specific component and said labelled analyte specific component, (g) washing said well following reaction of said analyte with said analyte specific component and said labelled analyte specific component, and (h) detecting a signal from said labelled analyte specific component; wherein all reagents other than said sample containing said analyte are added prior to step (e) and wherein said signal is a measure of said unknown amount of analyte in said sample.