Patent ID: 7488591
Filing Date: 2009-02-10
Classification: C12N,C12P

Abstract:
1. A method for controlling the formation of the acidic sugar chain in a yeast belonging to the genus Pichia , wherein a gene participating at least in the addition of mannose phosphate to a sugar chain in a core-like sugar chain of a glycoprotein is controlled, said gene comprising a nucleotide sequence which has at least 60 consecutive nucleotides of the coding region of the nucleotide sequence of SEQ ID NO: 2, wherein said coding region consists of nucleotides 150-2480 of SEQ ID NO: 2, wherein controlling the gene comprises at least one mutation, including deletion, substitution, insertion, or addition, into the gene, to reduce the ratio of acidic sugars to total sugars in the core-like sugar chain of the glycoprotein to 10% or less.