Patent ID: 6369011
Filing Date: 2002-04-09
Classification: C11D,C12N

Abstract:
A cleaning composition comprising:(a) from about 0.1% to about 10% by weight of protease enzyme which is a carbonyl hydrolase variant having an amino acid sequence not found in nature, which is derived from a precursor carbonyl hydrolase consisting of a substitution of a different amino acid for a plurality of amino acid residues at a position in said precursor carbonyl hydrolase equivalent to position +210 in Bacillus amyloliquefaciens subtilisin, in combination with one or more amino acid residue positions equivalent to those selected from the group consisting of +33, +62, +67, +76, +100, +101, +103, +104, +107, +128, +129, +130, +132, +135, +156, +158, +164, +166, +167, +170, +209, +215, +217, +218, and +222 in Bacillus amyloliquefaciens subtilisin, provided that: when said carbonyl hydrolase variant includes a substitution at positions equivalent to +210 and +76, there is also a substitution of an amino acid residue at one or more of said amino acid residue positions other than amino acid residue positions equivalent to positions +101, +103, +104, +107, +128, +135, +156, +166, +217, +218 and +222; and (b) one or more cleaning adjunct materials compatible with the protease enzyme.