Patent ID: 9200325
Filing Date: 2015-12-01
Classification: A61K,A61P,C12Q,G01N

Abstract:
1. A diagnostic screen for detecting and/or monitoring the progression of breast cancer, the diagnostic screen comprising the steps of: isolating a serum sample from a subject suspected of having breast cancer; isolating RNA in the serum sample; determining the concentration of SNORD44 small nuclear RNA (snRNA) in the subject's sample by amplifying the SNORD44 snRNA in the isolated RNA; determining the concentration of U6 snRNA in the subject's sample by amplifying the U6 snRNA in the isolated RNA; normalizing the concentration of the U6 snRNA detected in the subject's sample and the concentration of the U6 snRNA present in samples from healthy individuals, using SNORD44 as a normalization control; and detecting the presence of breast cancer if the normalized concentration of the U6 snRNA in the subject's sample is greater than the normalized concentration of the U6 snRNA in the samples from healthy individuals by a fold change of from about 2 to about 5.