Patent ID: 6888047
Filing Date: 2005-05-03
Classification: A01K,C07K,C12N

Abstract:
1. A method for producing a recombinant biologically active polypeptide, comprising: introducing an isolated DNA molecule comprising a mammalian uromodulin promoter operably linked to a heterologous DNA sequence encoding a heterologous polypeptide, wherein said mammalian uromodulin promoter directs expression of said heterologous polypeptide in vivo to the ascending limb of Henle's loop and the early distal tubules of the kidneys to produce a recombinant biologically active polypeptide in the urine, into a fertilized embryo of a non-human mammal selected from the group consisting of goat, cattle, sheep, pig, and mouse to generate a transgenic non-human mammal which expresses and secretes the heterologous polypeptide into the urine of the transgenic non-human mammal as a recombinant biologically active polypeptide; collecting urine from the transgenic non-human mammal; and recovering the secreted polypeptide to produce a recombinant biologically active polypeptide.