Patent ID: 7413863
Filing Date: 2008-08-19
Classification: G01N

Abstract:
1. A method of identifying a substance which inhibits mammalian NFκ-B Essential Modulator (NEMO) oligomerization, comprising providing a cell deficient in endogenous NEMO, and which is transformed with one or more polynucleotides that express at least two peptides that comprise a NEMO oligomerization region wherein the NEMO oligomerization region is at least one of coiled-coil motif or zinc finger moiety of the C-terminal domain of mammalian NEMO; applying the substance to the cell; and analyzing the level of oligomerization of the peptides that comprise a NEMO oligomerization region compared to the level of oligomerization of the peptides in the absence of the substance, wherein a reduction of oligomerization of the peptides in the presence of the substance indicates that the substance inhibits NEMO oligomerization wherein analyzing the level of oligomerization comprises a Fluorescence Resonance Energy Transfer protocol.