Patent ID: 6806089
Filing Date: 2004-10-19
Classification: G01N,Y10T

Abstract:
A method of measuring a concentration of an analyte in a sample, wherein said method comprises:a) irradiating said sample with modulated incident light wherein said sample comprises a first fluorophore which absorbs a portion of said incident light and then emits emitted light, wherein the portion of said incident light absorbed by said first fluorophore is sensitive to the concentration of the analyte in the sample; b) allowing said emitted light to irradiate a second fluorophore; c) measuring light emitted from said sample; d) determining the phase angle or modulation of said light emitted from said sample; and e) correlating said phase angle or modulation to said concentration of said analyte, wherein in said first fluorophore has a decay time on a nanosecond timescale, wherein said second fluorophore has a decay time on a microsecond timescale.