Patent ID: 6197258
Filing Date: 2001-03-06
Classification: G01N

Abstract:
A photoluminescent sensor for use the detection of a metal ion in an aqueous sample, said metal ion selected from the group consisting of Zn(II), Cu(II), Co(II), Ni(II), and Cd(II), said sensor comprising a macromolecule having a metal ion binding site, said macromolecule selected from the group consisting of human apocarbonic anhydrase, F131C variant of human apocarbonic anhydrase, and N67C variant of human apocarbonic anhydrase, said macromolecule having a photoluminescent label covalently bound thereto, said label selected from the group consisting of PyMPO, 1-[[(2',7'-difluoro-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H] xanthen]-5-yl)carbonyl]oxy]-2,5-pyrrolidinedione, and ABD-T.