Patent ID: 6440659
Filing Date: 2002-08-27
Classification: G01N

Abstract:
A method for screening an antiretroviral protease inhibitor for a metabolic side-effect on glucose transport, the method comprising the steps of:a) providing a reaction medium comprising one or more glucose transporters in a membrane system through which the glucose transporters transport a glucose compound; b) adding to the reaction medium a detectable form of the glucose compound; c) adding to the reaction medium the protease inhibitor to be screened; d) determining activity of the glucose transporter by measuring an amount of the glucose compound transported by the glucose transporter; and e) quantifying the metabolic side-effects of the protease inhibitor screened as a function of the activity of the glucose transporter in the presence the protease inhibitor as compared with the activity of the glucose transporter in the absence of the protease inhibitor.