Patent ID: 8758211
Filing Date: 2014-06-24
Classification: A61M,B04B

Abstract:
1. A method of selectively concentrating and collecting constituents of a biologic liquid mixture and applying a portion of said constituents to a scaffold material, said constituents having differing specific gravities and being stratifiable in a centrifugal field produced a centrifuge, said method comprising: a) providing the centrifuge having a chamber, an inlet, a first port and a second port, said chamber having a central longitudinal axis and being arranged to contain said biologic liquid mixture; b) introducing an entire volume of said biologic liquid mixture into said chamber; c) rotating said chamber about said central longitudinal axis to produce said centrifugal field, whereupon said constituents of said liquid mixture in said chamber stratify into at least two concentric stratified constituent layers as a function of the differing specific gravities of said constituent; d) selectively opening said first port to enable at least a portion of at least one of said at least two concentric stratified constituent layers to be discharged from said chamber through said first port thereby leaving a residual of said biologic liquid mixture in said chamber; and e) selectively opening said second port after said first port is opened to enable at least a portion of said residual liquid mixture to be discharged from said chamber through said second port and causing said at least a portion of said residual liquid mixture to engage said scaffold material.