Patent ID: 8343752
Filing Date: 2013-01-01
Classification: C12N,C12P,C12Y

Abstract:
1. A genetically modified Candida yeast, comprising: (a) a POX5 polypeptide; (b) a disruption, deletion or knockout of (i) a polynucleotide that encodes a POX4 polypeptide or (ii) a promoter operably linked to a polynucleotide that encodes a POX4 polypeptide, whereby POX4 activity is reduced or removed; (c) a disruption, deletion or knockout of (i) a polynucleotide that encodes a FAT1 polypeptide or (ii) a promoter operably linked to a polynucleotide that encodes a FAT1 polypeptide, whereby FAT1 activity is reduced or removed, which polynucleotide that encodes the FAT1 polypeptide comprises a nucleotide sequence that is 81% or more identical to SEQ ID NO: 50; and (d) a disruption, deletion or knockout of (i) a polynucleotide that encodes a ACS1 polypeptide or (ii) a promoter operably linked to a polynucleotide that encodes a ACS1 polypeptide, whereby the ACS1 activity is reduced or removed, which polynucleotide that encodes the ACS1 polypeptide comprises a nucleotide acid sequence that is 84% or more identical to SEQ ID NO: 48.