Patent ID: 6107030
Filing Date: 2000-08-22
Classification: C12Q

Abstract:
A method for determination of chromosomal orientation and strand direction of DNA sequences, which comprises the steps of:a. preparing a cell sample in which some cells have chromosomes in which a halogenated nucleotide analog has been incorporated into one strand of the DNA double helix;b. selectively removing the halogenated nucleotide analogue-substituted strand from the double helix by the steps of:i. inducing breaks in the halogenated nucleotide analogue-substituted strand; andii. denaturing the halogenated nucleotide analogue-substituted strand, whereby the halogenated nucleotide analogue-substituted strand is solubilized and the unsubstituted strand is left intact;c. hybridizing a single-stranded probe to the unsubstituted strand; andd. detecting the probe.