Patent ID: 6265169
Filing Date: 2001-07-24
Classification: C12Q,G01N

Abstract:
A method for detecting the presence of a molecule of interest in a biological samples, comprising a combination of the following operations:a) immobilising molecules of interest without destroying their ability to specifically interact with molecules exposed on the surfaces of a recombinant filamentous bacteriophage, which bacteriophage contains a single-strand DNA molecule whose sequence is at least partially known;b) causing an immobilised molecule of interest to bind specifically with at least one of the molecules exposed on the surface of at least one of a plurality of recombinant filamentous bacteriophages in a reaction mixture to form an immobilised complex between said molecule of interest and said at least one bacteriophage;c) separating said immobilised complex from the reaction mixture;d) washing to remove any complexes generated by non-specific interaction between said molecule of interest and bacteriophage;e) adding the reagents to carry out the operation described under g);f) lysing the proteins forming the protein capsid of said bacteriophage of said immobilised complex;g) amplifying the DNA obtained from lysing said bacteriophage;h) detecting the amplified DNA.