Patent ID: 6791014
Filing Date: 2004-09-14
Classification: C12N

Abstract:
Method for transforming plant cells by introducing a heterologous gene into said plant cells with a gene for tolerance to HPPD inhibitors as a selection marker, said method comprising the steps of:(a) preparing and culturing competent plant cells capable of receiving the heterologous gene and selection marker in a suitable medium, wherein said competent plant cells are embryogenic calluses or embryogenic tissues, (b) bleaching the competent plant cells by introducing a suitable amount of HPPD inhibitor into the suitable medium of the competent plant cells, (c) transforming the competent cells with the heterologous gene and the selection marker, (d) growing and selecting the transformed cells comprising the heterologous gene and the selection marker in a suitable medium wherein transformed cells appear green indicating the presence in the transformed cells of the heterologous gene and selection marker, wherein said gene for tolerance to HPPD inhibitors comprises, in the direction of transcription, a regulatory promoter sequence which is functional in plant cells and plants, functionally linked to a DNA sequence encoding an HPPD, functionally linked to a regulatory terminator sequence which is functional in plant cells and plants.