Patent ID: 8623598
Filing Date: 2014-01-07
Classification: C12Q,G16B

Abstract:
1. A method of incorporating labeled nucleotides into nucleic acid, comprising: a) providing a plurality of nucleic acid template molecules, a polymerase, a cleaving agent, a cleaving agent scavenger comprising a disulfide bond, and a plurality of nucleotide analogues wherein each nucleotide analogue is labeled with a unique label attached through a cleavable disulfide linker and contains a removable chemical moiety capping the 3′-OH group; b) incorporating a first nucleotide analogue with said polymerase; c) detecting the label of the incorporated nucleotide analogue; d) removing the chemical moiety of the incorporated nucleotide analogue capping the 3′-OH group with said cleaving agent; and e) incorporating a second nucleotide analogue in the presence of said cleaving agent scavenger.