Patent ID: 6461617
Filing Date: 2002-10-08
Classification: A61K,A61P,C07K,C12N,Y02A,Y10S

Abstract:
A non-toxic polypeptide comprising first, second and third domains, wherein(a) said first domain comprises a botulinum toxin light chain and cleaves one or more vesicle or plasma-membrane associated proteins essential to exocytosis, (b) said second domain comprises the first 100 N-terminal amino acids of a botulinum toxin heavy chain and (i) translocates the polypeptide into a cell or (ii) increases the solubility of the polypeptide compared to the solubility of the first domain on its own or (iii) both translocates the polypeptide into a cell and increases the solubility of the polypeptide compared to the solubility of the first domain on its own, (c) said polypeptide is free of clostridial neurotoxin and free of clostridial neurotoxin precursor that can be converted into toxin by proteolytic action, (d) said polypeptide is a single polypeptide, (e) said third domain is a tandem repeat synthetic IgG binding domain derived from domain &bgr; of Staphylococcal protein A, and (f) said polypeptide lacks a portion designated Hc of a botulinum toxin heavy chain.