Patent ID: 6653153
Filing Date: 2003-11-25
Classification: B01J,C07B,C40B,G01N,Y10T

Abstract:
A method of preparing an encoded combinatorial library comprising a plurality of ligand-bearing support units, wherein said ligand-bearing support units comprise (a) a solid carrier, (b) one or more ligands covalently bound to said solid carrier, and (c) a plurality of encoding metal salts impregnated on said support unit, said metal salts providing a code for identifying said ligand, comprising the steps of:(1) providing support units comprising a solid carrier having functional groups, said functional groups being optionally connected to linker groups, said linker groups being organic residues covalently bound to the functional groups of said solid carrier and having functional groups for covalent binding to said ligand; (2) covalently attaching a ligand or a first sub-unit of a ligand that will have more than one sub-unit to the functional group of said carrier or to the functional group of an optional linker group, said sub-unit having a functional group for covalent binding to a second sub-unit; (3) impregnating said support unit with one or more encoding metal salts, said salts being comprised of one or more encoding metal cations, said encoding metal cations being distributed in their natural isotope abundance or in a non-natural isotope abundance, wherein the encoding metal cations provide a code for identifying the ligand or sub-unit that is attached to said support unit, wherein the encoding metal salts are impregnated onto the support unit by treating the support unit with a solution of a soluble salt of the encoding metal cations to form a support unit comprising the soluble encoding metal cations, and then treating said support unit comprising said soluble encoding metal salts with a solution of a salt having an anion that forms insoluble or poorly soluble salts when combined with said encoding metal salts, thereby yielding a stabilized encoding metal salt, said salt being stabilized against dissolution from said support unit; wherein step (3) is optionally carried out before or after step (2) or concurrently with step (2).