Patent ID: 7306902
Filing Date: 2007-12-11
Classification: A61P,G01N,Y10S

Abstract:
1. A method of diagnosing a neoplasm in an animal by phenotype, comprising: (a) providing a biological sample from the animal, wherein the sample comprises neoplastic cells with one or more unknown phenotypes; (b) providing at least two oncolytic viruses, wherein each oncolytic virus selectively replicates in neoplastic cells having a known phenotype selected from the group consisting of ras pathway activation, interferon-resistance, p53-deficiency and Rb-deficiency; and wherein each of the at least two oncolytic viruses replicates in neoplastic cells having a known different phenotype; (c) contacting the neoplastic cells from the sample with each of the at least two oncolytic viruses under conditions which allow each oncolytic virus to selectively replicate in the neoplastic cells from the sample; (d) determining if each of the oncolytic viruses selectively replicates in the neoplastic cells from the sample; and (e) diagnosing a neoplasm in the animal as comprising cells of a known phenotype according to the ability of each of the oncolytic viruses to selectively replicate in the neoplastic cells from the sample.