Patent ID: 7332271
Filing Date: 2008-02-19
Classification: B01F,B01J,B01L,C40B,H01J,Y02P

Abstract:
1. A method for simultaneously conducting a plurality of micro-volume polynucleotide amplification reactions in a microhole device, the method comprising: (a) providing a microhole device having a substrate, wherein the substrate is solid and defines a plurality of sample chambers for retaining liquid samples through surface tension wherein each sample chamber comprises a wall and an opening at each end such that a liquid sample present in one sample chamber does not intermix with a liquid sample present in another sample chamber, each sample chamber containing at least one desired polynucleotide amplification reaction component, wherein such component having the possibility of being distinct from a reaction component in a different sample chamber; (b) introducing a liquid sample containing at least one additional desired polynucleotide amplification reaction component into the sample chambers of the microhole device; (c) submerging the device in a hydrophobic medium; and (d) placing the submerged device into any environment favorable to the polynucleotide amplification reaction.