Patent ID: 8247235
Filing Date: 2012-08-21
Classification: C07K,G01N,G16B,G16H,Y10T

Abstract:
1. A method for purifying lipoproteins suitable for differential charged-particle mobility analysis of lipoprotein class and subclass, said method comprising: (a) preparing a centrifuge tube containing a first solution underneath a sample and a second solution above said sample, said sample comprising high density lipoproteins (HDL) and non-lipoprotein components, said first solution having a first density greater than 1.00 g/mL and less than or equal to about 1.21 g/mL, and said second solution having a second density greater than or equal to 1.00 g/mL and less than said first density; (b) subjecting said tube to centrifugation sufficient to cause said non-lipoprotein components to migrate toward the bottom of the tube and away from said lipoproteins, thereby providing purified lipoproteins; wherein said sample further comprises dextran sulfate under conditions wherein said HDL are not precipitated from said sample.