Patent ID: 8932815
Filing Date: 2015-01-13
Classification: B03C,C12N,C12Q,G01N

Abstract:
1. A method for quantifying nucleic acid in a sample, the method comprising: a. applying a sample with a conductivity of greater than 100 mS/m to a device, the device comprising an array of electrodes capable of establishing an AC electrokinetic field region, the device further comprising at least two chambers, the sample applied to a first chamber; b. applying known quantities of a nucleic acid standard to a second chamber; c. establishing a first AC electrokinetic high field region in the first chamber, the first AC electrokinetic high field capable of isolating larger nanoparticulate molecular targets; d. establishing a second AC electrokinetic low field in the first chamber, the second AC electrokinetic low field capable of concentrating cells or micron-sized entities that may be present in the sample; e. establishing a third AC electrokinetic high field to the second chamber, the second AC electrokinetic high field isolating the molecular nucleic acid standard applied to the second chamber; f. flushing cells and micron-sized entities that may be present in the sample from the first chamber; g. detecting bound nucleic acid signal on the array in the first chamber and second chamber; and h. quantifying the bound nucleic acid by comparing the detected signal from the first chamber to detected signal from the second chamber.