Patent ID: 7569363
Filing Date: 2009-08-04
Classification: C07K,C12N,Y02A

Abstract:
1. A method of making a modified Cry3A toxin, said method comprising: (a) obtaining a cry3A coding sequence which encodes a Cry3A toxin; (b) obtaining a nucleotide sequence which encodes a cathepsin G recognition site; (c) inserting the nucleotide sequence into the cry3A coding sequence, such that the cathepsin G recognition site is located in the encoded Cry3A toxin at a position between amino acids corresponding to amino acid numbers 107 and 115 of SEQ ID NO: 4, thus creating a modified Cry3A coding sequence; (d) inserting the modified cry3A coding sequence into an expression cassette; (e) transforming the expression cassette into a non-human host cell; and (f) growing the host cell of step (e) under conditions wherein the host cell produces a modified Cry3A toxin.