Patent ID: 6153383
Filing Date: 2000-11-28
Classification: C07K,C12N,G01N

Abstract:
A method for identifying a transcriptional modulator, comprising:providing a cell comprising (i) a genetic construct encoding a chimeric protein which comprises at least one ligand-binding domain and a DNA-binding domain which is heterologous thereto, wherein the ligand-binding domain binds to a selected ligand, and (ii) a target gene under the control of at least one transcriptional regulatory element which is recognized by the DNA-binding domain of the chimeric protein;contacting the cell with a test compound which comprises the selected ligand linked to a test transcriptional modulating portion under conditions which allow transcription to occur; anddetecting any changes in transcriptional activity of the target gene in the presence of the test compound relative to that detected in the absence of the test compound,wherein a change in the level of transcriptional activity of the target gene detected in the presence of the test compound relative to that detected in the absence of the test compound indicates that the test compound is a transcriptional modulator.