Patent ID: 7547383
Filing Date: 2009-06-16
Classification: C12Q,Y10T

Abstract:
1. A method of using an electrode strip for determining the concentration of creatinine, said method comprising: obtaining an electrode strip comprising a first working electrode with a reagent matrix capable of measuring creatine in a liquid sample, a second working electrode with a reagent matrix capable of measuring creatine and creatinine in said liquid sample and a reference electrode wherein said reagent matrix of said first working electrode and said second working electrode are water-soluble and contain a peroxidase enzyme; disposing a fluid sample over said first working electrode, said second working electrode and said reference electrode of said electrode strip; applying a potential of 0.0V or less between said reference electrode and said first working electrode; measuring a first current generated between said first working electrode and said reference electrode and correlating said first current to a concentration of said creatine in said fluid sample; applying a potential between said reference electrode and said second working electrode; measuring a second current generated between said second working electrode and said reference electrode; subtracting said first current from said second current and obtaining a current difference whereby said current difference is representative of the concentration of creatinine in said fluid sample.