Patent ID: 6251597
Filing Date: 2001-06-26
Classification: A01K,A61K,C07K,C12N

Abstract:
A method for determining whether fohy030 mRNA is present in a test sample, the method comprising:a) obtaining a test sample comprising mRNA from a test subject;b) contacting the test sample with an isolated nucleic acid molecule that hybridizes under conditions of hybridization at 65.degree. C. in 0.5 M NaHPO.sub.4, 7% SDS and washing at 68.degree. C. in 0.1.times.SSC, 0.1% SDS to the fohy030 mRNA; andc) determining that the fohy030 mRNA is present in the sample when the sample contains mRNA that selectively hybridizes to the isolated nucleic acid molecule;wherein the fohy030 mRNA is selected from the group consisting of:i) an mRNA molecule that encodes a polypeptide comprising the amino acid sequence of SEQ ID NO.7;ii) an mRNA molecule of a DNA molecule comprising the nucleotide sequence of SEQ ID NO:6;iii) an mRNA molecule that hybridizes under conditions of hybridization at 65.degree. C. in 0.5 M NaHPO.sub.4, 7% SDS and washing at 68.degree. C. in 0.1.times.SSC, 0.1% SDS to a nucleic acid molecule consisting of the nucleotide sequence of SEQ ID NO:6 or the complement thereof;iv) an mRNA molecule that hybridizes under conditions of hybridization at 65.degree. C. in 0.5 M NaHPO.sub.4, 7% SDS and washing at 68.degree. C. in 0.1.times.SSC, 0.1% SDS to a nucleic acid molecule consisting of the nucleotide sequence of the cDNA insert of the plasmid deposited with ATCC as Accession Number 97880, or the complement thereof; andv) an mRNA molecule of a DNA molecule that comprises 30 contiguous nucleotides of SEQ ID NO:6.