Patent ID: 6297023
Filing Date: 2001-10-02
Classification: C12Q,G01N

Abstract:
A method for obtaining an initial activity of plasminogen activator inhibitor-1, an initial activity of tissue plasminogen activator, or a half-life of tissue plasminogen activity in a sample comprising the steps of:(a) measuring activity of tissue plasminogen activator in the sample as a function of time; and(b) obtaining a value of (PAI).sub.0, (t-PA).sub.0 or 1/2 (t-PA).sub.0 ; from formula:kt=-1/((PAI).sub.0 -(tPA).sub.0)ln{(tPA).sub.0 [(PAI).sub.0 -(tPA).sub.0 +(tPA)]}/(PAI).sub.0 (twherein (PAI).sub.0 is the initial activity of plasminogen activator inhibitor-1 in the sample;(tPA).sub.0 is the initial activity of tissue plasminogen activator in the sample;(tis an activity of tissue plasminogen activator at time t; andthe half-life of tissue plasminogen activator in the sample is a value for t wherein (tis 1/2 (t-PA).sub.0 ;k is a second order rate constant for a reaction: ##STR3##