Patent ID: 7452986
Filing Date: 2008-11-18
Classification: C12N

Abstract:
1. A DNA construct for stably transforming the plastids of higher plants, comprising: a) a transcription unit encoding at least one exogenous protein of interest; and b) a NEP promoter and a PEP promoter in tandem, operably linked to said transcription unit, wherein expression of said transcription unit is regulated by said promoters, wherein said NEP promoter is the NEP promoter from a gene selected from the group consisting of clpP, rpoB, and atpB, wherein said atpB gene is from a plant selected from the group consisting of maize, sorghum, barley, wheat, and rice, wherein said rpoB gene is selected from the group consisting of maize, and rice, and wherein said clpP gene is from a plant selected from the group consisting of maize, rice, and wheat; and wherein said PEP promoter is Prrn or is the clpP PEP promoter from rice.