Patent ID: 6844161
Filing Date: 2005-01-18
Classification: A61K,C07K

Abstract:
1. A method of producing a cross-over chemokine protein that contains at least one peptide segment whose sequence is derived from a first chemokine protein, and at least one peptide segment whose sequence is derived from a second chemokine protein, wherein said second chemokine protein has an amino acid sequence that is different from that of said first chemokine protein, and wherein each of said peptide segments possesses an N-terminal amino acid residue and a C-terminal amino acid residue, said method comprising: ligating under chemoselective chemical ligation conditions (i) at least one peptide segment comprising a functional protein module derived from said first chemokine protein, and (ii) at least one peptide segment comprising a functional protein module derived from said second chemokine protein, wherein each of said peptide segments exhibit sufficient homology to a functional protein module of a chemokine to permit said peptide segments to mediate the function of the chemokine functional protein module when incorporated into said cross-over chemokine protein, and wherein the C-terminal residue of said peptide segment derived from said first chemokine protein and the N-terminal residue of said peptide segment derived from said second chemokine protein comprise compatible reactive groups capable of chemoselective chemical ligation to one another, whereby a covalent bond is formed between said compatible reactive groups of said peptide segments so as to produce a chemical ligation product comprising a cross-over chemokine protein in which the C-terminal residue of the peptide segment derived from said first chemokine protein is ligated to the N-terminal residue of said peptide segment derived from said second chemokine protein.