Patent ID: 9176123
Filing Date: 2015-11-03
Classification: C12Q,G01N

Abstract:
1. A binding-induced hairpin detection system comprising: a. a first probe comprising a targeting molecule and an oligonucleotide that has a free end comprising a hairpin loop and a hairpin stem and an end attached to the targeting molecule; and b. a second probe comprising a targeting molecule and an oligonucleotide that has an end attached to the targeting molecule and a free end comprising a nucleotide sequence that is complementary to a nucleotide sequence at or near the free end of the oligonucleotide of the first probe; wherein the targeting molecule of the first and second probe is independently selected from a peptide aptamer, a protein receptor molecule, an antibody, or a ligand that is a peptide, a hormone, a lipid, or a glycan; and wherein upon binding of the targeting molecule to a target molecule, the free end of the oligonucleotide of the second probe hybridizes at or near the free end of the oligonucleotide of the first probe forming, a hairpin stem, the non-hybridized portions of the first and second probes together with the target molecule bound thereto forming a hairpin loop, thereby providing a binding-induced hairpin with a closed circular structure.