Patent ID: 8563240
Filing Date: 2013-10-22
Classification: C12Q

Abstract:
1. A method for determining sequences of a sample of DNAs to be sequenced comprising, providing an array comprising a plurality of regions, and a sensor; hybridizing primer strands of DNA to the DNAs to be sequenced wherein the hybridized primer strands of DNA are terminated with a nuclease-resistant nucleotide, and wherein statistically one DNA to be sequenced is immobilized in one region; providing a first set of reactants to the regions, the first set of reactants comprising incorporating the nuclease-resistant blocking nucleotides into primer strands hybridized to those DNAs to be sequenced whose next unpaired base is complementary to the only one type of base comprised by the nuclease-resistant blocking nucleotides; providing a second set of reactants to the regions, the second set of reactants comprising extending hybridized primer strands to which the nuclease-resistant blocking nucleotides are not incorporated, with the nucleotides and/or the analogs of nucleotides; detecting with the sensor increases in reaction products in the regions resulting from the extending hybridized primer strands with the nucleotides and/or the analogs of nucleotides; removing the nucleotides and/or the analogs of nucleotides that extended the hybridized primer strands, from the hybridized primer strands; and determining sequence information for the sample of DNAs to be sequenced based on detected increases in reaction products by the sensor.