Patent ID: 8420325
Filing Date: 2013-04-16
Classification: C12N,C12Q

Abstract:
1. An isolated thermostable Taq DNA polymerase mutant having altered fidelity, wherein said mutant comprises one or more mutated amino acid residues in the active site O-helix of a thermostable polymerase, wherein said mutated amino acid residue is adjacent to an immutable or nearly immutable residue corresponding to Arg659, Lys663, Phe667 or Tyr671 of SEQ ID NO:2, wherein at least one mutation comprises a residue corresponding to Phe667Leu, and, optionally, additional mutations selected from the group of residues corresponding to: Asn666Asp; Asn666Ile; Ile665Leu; Leu670Val; Arg660Tyr; Arg660Ser; Gly668Arg; Arg660Lys; Gly668Ser; Gly668Gln; Thr664Ile and Asn666Asp; Ala661Ser and Val669Leu; Ala661Glu, and Ile665Thr; and Thr664Pro, Ile665Val and Asn666Tyr of SEQ ID NO:2; and said Arg659 and Lys663 residues are not mutated.