Patent ID: 6599441
Filing Date: 2003-07-29
Classification: B01F,B01L,C30B,Y10T

Abstract:
A set of crystallization solutions comprising a crystallization solution set selected from the group of crystallization solution sets consisting of:SolutionCompound BufferPrecipitantSalt(a) Crystallization Solution Set 1:1100.000 mM HEPES/NaOH pH:10.000% v/v200.000 mM7.5isopropanolsodiumchloride2100.000 mM Na3 citrate/citric acid20.000% w/vpH: 5.5PEG-30003100.000 mM MES/NaOH pH: 6.10.000% w/v200.000 mMPEG-8000ZnAc24100.000 mM Na3 citrate/citric acid2000.000pH: 5.5mM (NH4)2sulfate5100.000 mM Tris base/HCl pH: 7.20.000% w/vPEG-2000MME6100.000 mM MES/NaOH pH: 6.20.000% v/v200.000 mM1,4-butane-Li2 sulfatediol7100.000 mM imidazole/HCl pH: 8.20.000% w/v200.000 mMPEG-1000CaAc28100.000 mM Na2 H phosphate/K2500.000H2 phosphate pH: 6.2mM sodiumchloride9100.000 mM acetic acid/NaAc pH:30.000% w/v200.000 mM4.5PEG-8000Li2 sulfate10100.000 mM Tris base/HCl pH:10.000% v/v8.5isopropanol11100.000 mM Tris base/HCl pH:30.000% v/v200.000 mM8.5PEG-400MgCl212100.000 mM imidazole/HCl pH: 8.2500.000mM sodiumchloride13100.000 mM MES/NaOH pH: 6.10.000% v/v200.000 mMisopropanolCaAc214100.000 mM Tris base/HCl pH: 7.15.000% v/vEtOH15100.000 mM acetic acid/NaAc pH:30.000% v/v200.000 mM4.5PEG-400CaAc216100.000 mM acetic acid/NaAc pH:20.000% w/v200.000 mM4.5PEG-1000ZnAc217100.000 mM acetic acid/NaAc pH:10.000% w/v200.000 mM4.5PEG-3000ZnAc218100.000 mM Na2 H phosphate/K10.000% w/v200.000 mMH2 phosphate pH: 6.2PEG-8000sodiumchloride19100.000 mM Tris base/HCl pH:1000.0008.5mM (NH4)2H phosphate20100.000 mM Na cacodylate/HCl10.000% v/v200.000 mMpH: 6.5isopropanolZnAc221100.000 mM Na cacodylate/HCl30.000% v/v200.000 mMpH: 6.5PEG-400Li2 sulfate22100.000 mM Na3 citrate/citric acid15.000% v/v200.000 mMpH: 5.5EtOHLi2 sulfate23100.000 mM HEPES/NaOH pH:1260.0007.5mM (NH4)2sulfate24100.000 mM Tris base/HCl pH: 7.2500.000200.000 mMmM sodiumMgCl2chloride25100.000 mM MES/NaOH pH: 6.15.000% v/v200.000 mMEtOHZnAc226100.000 mM imidazole/HCl pH: 8.10.000% v/visopropanol27100.000 mM HEPES/NaOH pH:15.000% v/v200.000 mM7.5EtOHMgCl228100.000 mM Na cacodylate/HCl10.000% w/v200.000 mMpH: 6.5PEG-3000MgCl229100.000 mM MES/NaOH pH: 6.20.000% w/v200.000 mMPEG-8000CaAc230100.000 mM CHES/NaOH pH:1260.000200.000 mM9.5mM (NH4)2sodiumsulfatechloride31100.000 mM imidazole/HCl pH: 8.20.000% v/v200.000 mM1,4-butane-ZnAc2diol32100.000 mM Tris base/HCl pH: 7.1000.000200.000 mMmM Na3sodiumcitratechloride33100.000 mM HEPES/NaOH pH:30.000% v/v200.000 mM7.5PEG-400sodiumchloride34100.000 mM Tris base/HCl pH: 7.10.000% w/v200.000 mMPEG-8000MgCl235100.000 mM Na cacodylate/HCl20.000% w/v200.000 mMpH: 6.5PEG-1000MgCl236100.000 mM imidazole/HCl pH: 8.2500.000200.000 mMmM sodiumZnAc2chloride37100.000 mM Na2 H phosphate/K40.000% v/vH2 phosphate pH: 6.2EG38100.000 mM Tris base/HCl pH:30.000% v/v200.000 mM8.5PEG-200(NH4)2 Hphosphate39100.000 mM HEPES/NaOH pH:10.000% v/v50.000 mM7.5glycerolLi2 sulfate30.000% v/vPEG-60040100.000 mM HEPES/NaOH pH:40.000% v/v7.5EG5.000% w/vPEG-300041100.000 mM Tris base/HCl pH: 7.40.000% v/vEG42100.000 mM Tris base/HCl pH:50.000% v/v200.000 mM8.5EGMgCl243100.000 mM Tris base/HCl pH:5.000% w/v8.5PEG-800010.000% v/vglycerol20.000% v/vPEG-30044100.000 mM acetic acid/NaAc pH:40.000% v/v4.5PEG-40045100.000 mM Tris base/HCl pH: 7.10.000% v/v500.000 mMglycerol(NH4)230.000% v/vsulfatePEG-60046100.000 mM HEPES/NaOH pH:50.000% v/v200.000 mM7.5EGLi2 sulfate47100.000 mM acetic acid/NaAc pH:30.000% v/v100.000 mM4.5PEG-200sodiumchloride48100.000 mM CAPS/NaOH pH:30.000% v/v200.000 mM10.5PEG-200(NH4)2sulfate(b) Crystallization Solution Set 2:1100.000 mM Tris base/HCl pH: 7.10.000% v/v200.000 mMisopropanolZnAc22100.000 mM acetic acid/NaAc pH:10.000% v/v200.000 mM4.5isopropanolCaAc23100.000 mM Na3 citrate/Na310.000% v/v200.000 mMcitrate pH: 5.5isopropanolLi2 sulfate4100.000 mM Tris base/HCl pH: 7.10.000% v/v200.000 mMisopropanolCaAc25100.000 mM acetic acid/NaAc pH:10.000% v/v200.000 mM4.5isopropanolsodiumchloride6100.000 mM Na3 citrate/Na310.000% v/v200.000 mMcitrate pH: 5.5isopropanolMgCl27100.000 mM HEPES/NaOH pH:10.000% w/v200.000 mM7.5PEG-8000sodiumchloride8100.000 mM MES/NaOH pH: 6.10.000% w/v200.000 mMPEG-8000ZnAc29100.000 mM Na2 H phosphate/K10.000% w/v200.000 mMH2 phosphate pH: 6.2PEG-8000Li2 sulfate10100.000 mM acetic acid/NaAc pH:10.000% w/v200.000 mM4.5PEG-8000CaAc211100.000 mM Tris base/HCl pH:10.000% w/v200.000 mM8.5PEG-8000MgCl212100.000 mM Na cacodylate/HCl10.000% w/v200.000 mMpH: 6.5PEG-8000sodiumchloride13100.000 mM Na3 citrate/citric acid10.000% w/v200.000 mMpH: 5.5PEG-8000Li2 sulfate14100.000 mM HEPES/NaOH pH:10.000% w/v200.000 mM7.5PEG-8000MgCl215100.000 mM HEPES/NaOH pH:20.000% w/v200.000 mM7.5PEG-2000NaClMME16100.000 mM MES/NaOH pH: 6.20.000% w/v200.000 mMPEG-2000ZnAc2MME17100.000 mM Na2 H phosphate/K20.000% w/vH2 phosphate pH: 6.2PEG-2000MME200.000 mMLi2 sulfate18100.000 mM acetic acid/NaAc pH:20.000% w/v200.000 mM4.5PEG-2000CaAc2MME19100.000 mM Tris base/HCl pH:20.000% w/v200.000 mM8.5PEG-2000MgCl2MME20100.000 mM cacodylic acid/Na20.000% w/vcacodylate pH: 6.5PEG-2000MME200.000 mMLi2 sulfate21100.000 mM HEPES/NaOH pH:20.000% w/v7.5PEG-2000MME200.000 mMLi2 sulfate22100.000 mM Tris base/HCl pH:20.000% v/v8.51,4-butane-diol 200.000mM Li2 sul-fate23100.000 mM cacodylic acid/Na20.000% v/v200.000 mMcacodylate pH: 6.51,4-butane-CaAc2diol24100.000 mM Na3 citrate/citric acid20.000% v/v200.000 mMpH: 5.51,4-butane-MgCl2diol(c) Crystallization Solution Set 3:1100.000 mM Tris base/HCl pH: 7.20.000% v/v200.000 mM1,4-butane-ZnAc2diol2100.000 mM Tris base/HCl pH:20.000% v/v200.000 mM8.51,4-butane-MgCl2diol3100.000 mM Na3 citrate/citric acid20.000% v/v200.000 mMpH: 5.51,4-butane-sodiumdiolchloride4100.000 mM HEPES/NaOH pH:20.000% w/v200.000 mM7.5PEG-1000sodiumchloride5100.000 mM MES/NaOH pH: 620.000% w/v200.000 mMPEG-1000ZnAc26100.000 mM Tris base/HCl pH: 7.20.000% w/v200.000 mMPEG-1000Li2 sulfate7100.000 mM HEPES/NaOH pH:20.000% w/v200.000 mM7.5PEG-1000MgCl28100.000 mM MES/NaOH pH: 6.20.000% w/v200.000 mMPEG-1000sodiumchloride9100.000 mM HEPES/NaOH pH:2500.0007.5mM sodiumchloride10100.000 mM MES/NaOH pH: 6.2500.000200.000 mMmM sodiumZnAc2chloride11100.000 mM Tris base/HCl pH:2500.000200.000 mM8.5mM sodiumLi2 sulfatechloride12100.000 mM cacodylic acid/Na2500.000200.000 mMcacodylate pH: 6.5mM sodiumCaAc2chloride13100.000 mM Na3 citrate/citric acid2500.000200.000 mMpH: 5.5mM sodiumMgCl2chloride14100.000 mM cacodylic acid/Na2500.000200.000 mMcacodylate pH: 6.5mM sodiumLi2 sulfatechloride15100.000 mM Tris base/HCl pH: 7.30.000% v/v200.000 mMPEG-400ZnAc216100.000 mM imidazole/HCl pH: 8.30.000% v/v200.000 mMPEG-400Li2 sulfate17100.000 mM Na3 citrate/citric acid30.000% v/v200.000 mMpH: 5.5PEG-400MgCl218100.000 mM Na3 citrate/citric acid30.000% v/v200.000 mMpH: 5.5PEG-400Li2 sulfate19100.000 mM imidazole/HCl pH: 8.30.000% v/v200.000 mMPEG-400ZnAc220100.000 mM Tris base/HCl pH: 7.30.000% v/v200.000 mMPEG-400NaCl21100.000 mM Na2 H phosphate/K15.000% v/v200.000 mMH2 phosphate pH: 6.2EtOHsodiumchloride22100.000 mM acetic acid/NaAc pH:15.000% v/v200.000 mM4.5EtOHZnAc223100.000 mM cacodylic acid/Na15.000% v/v200.000 mMcacodylate pH: 6.5EtOHCaAc224100.000 mM acetic acid/NaAc pH:15.000% v/v200.000 mM4.5EtOHMgCl2(d) Crystallization Solution Set 4:1100.000 mM cacodylic acid/Na15.000% v/v200.000 mMcacodylate pH: 6.5EtOHsodiumchloride2100.000 mM HEPES/HEPES pH:10.000% w/v200.000 mM7.5PEG-3000sodiumchloride3100.000 mM MES/NaOH pH: 6.10.000% w/v200.000 mMPEG-3000ZnAc24100.000 mM Tris base/HCl pH:10.000% w/v200.000 mM8.5PEG-3000CaAc25100.000 mM Na3 citrate/citric acid10.000% w/v200.000 mMpH: 5.5PEG-3000MgCl26100.000 mM Tris base/HCl pH:10.000% w/v200.000 mM8.5PEG-3000Li2 sulfate7100.000 mM MES/NaOH pH: 6.10.000% w/v200.000 mMPEG-3000Li2 sulfate8100.000 mM HEPES/HEPES pH:1000.000200.000 mM7.5mM (NH4)2sodiumH phosphatechloride9100.000 mM cacodylic acid/Na1000.000200.000 mMcacodylate pH: 6.5mM (NH4)2sodiumH phosphatechloride10100.000 mM Na2 H phosphate/K1260.000200.000 mMH2 phosphate pH: 6.2mM (NH4)2Li2 sulfatesulfate11100.000 mM Tris base/HCl pH: 7.1260.000200.000 mMmM (NH4)2MgCl2sulfate12100.000 mM imidazole/HCl pH: 8.1260.000200.000 mMmM (NH4)2Li2 sulfatesulfate13100.000 mM Na3 citrate/citric acid1260.000200.000 mMpH: 5.5mM (NH4)2sodiumsulfatechloride14100.000 mM HEPES/HEPES pH:20.000% w/v200.000 mM7.5PEG-8000sodiumchloride15100.000 mM Tris base/HCl pH: 7.20.000% w/v200.000 mMPEG-8000ZnAc216100.000 mM imidazole/HCl pH: 8.20.000% w/v200.000 mMPEG-8000Li2 sulfate17100.000 mM acetic acid/NaAc pH:20.000% w/v200.000 mM4.5PEG-8000MgCl218100.000 mM cacodylic acid/Na20.000% w/v200.000 mMcacodylate pH: 6.5PEG-8000sodiumchloride19100.000 mM Tris base/HCl pH: 7.20.000% w/v200.000 mMPEG-8000MgCl220100.000 mM HEPES/HEPES pH:1000.000200.000 mM7.5mM Na3sodiumcitratechloride21100.000 mM MES/NaOH pH: 6.1000.000200.000 mMmM Na3ZnAc2citrate22100.000 mM imidazole/HCl pH: 8.1000.000200.000 mMmM Na3Li2 sulfatecitrate23100.000 mM acetic acid/NaAc pH:1000.000200.000 mM4.5mM Na3MgCl2citrate24100.000 mM Tris base/HCl pH:1000.000200.000 mM8.5mM Na3Li2 sulfatecitrate(e) Crystallization Solution Set 5:1100.000 mM MES/NaOH pH: 6.1000.000200.000 mMmM Na3MgCl2citrate2100.000 mM acetic acid/NaAc pH:10.000% v/v200.000 mM4.5isopropanolLi2 sulfate3100.000 mM Na3 citrate/citric acid10.000% v/v200.000 mMpH: 5.5isopropanolsodiumchloride4100.000 mM acetic acid/NaAc pH:10.000% w/v200.000 mM4.5PEG-8000MgCl25100.000 mM Tris base/HCl pH:10.000% w/v200.000 mM8.5PEG-8000sodiumchloride6100.000 mM acetic acid/NaAc pH:20.000% w/v200.000 mM4.5PEG-2000ZnAc2MME7100.000 mM Tris base/HCl pH:20.000% w/v200.000 mM8.5PEG-2000Li2 sulfateMME8100.000 mM Tris base/HCl pH:20.000% w/v200.000 mM8.51,4-butane-sodiumdiolchloride9100.000 mM cacodylic acid/Na20.000% w/v200.000 mMcacodylate pH: 6.51,4-butane-Li2 sulfatediol10100.000 mM HEPES/NaOH pH:20.000% w/v200.000 mM7.5PEG-1000Li2 sulfate11100.000 mM MES/NaOH pH: 6.20.000% w/v200.000 mMPEG-1000MgCl212100.000 mM MES/NaOH pH: 6.2500.000200.000 mMnM sodiumLi2 sulfatechloride13100.000 mM Tris base/HCl pH:30.000% w/v200.000 mM8.5PEG-8000Li2 sulfate14100.000 mM cacodylic acid/Na30.000% w/v200.000 mMcacodylate pH: 6.5PEG-8000CaAc215100.000 mM MES/NaOH pH: 6.30.000% w/v200.000 mMPEG-400MgCl216100.000 mM Tris base/HCl pH: 7.30.000% w/v200.000 mMPEG-400Li2 sulfate17100.000 mM HEPES/NaOH pH:10.000% w/v200.000 mM7.5PEG-3000Li2 sulfate18100.000 mM MES/NaOH pH: 6.10.000% w/v200.000 mMPEG-3000MgCl219100.000 mM imidazole/HCl pH: 8.1000.000200.000 mMmM Na3MgCl2citrate20100.000 mM Tris base/HCl pH:1000.000200.000 mM8.5mM Na3sodiumcitratechloride21100.000 mM Tris base/HCl pH: 7.2500.000mM NaMal22100.000 mM acetic acid/NaAc pH:2500.0004.5mM NaMal23100.000 mM Tris base/HCl pH:2500.0008.5mM NaMal24100.000 mM imidazole/HCl pH: 8.2500.000mM NaMal.