Patent ID: 7875453
Filing Date: 2011-01-25
Classification: C12N

Abstract:
1. A method of producing a population of cells having a mature hepatocyte phenotype, said method comprising a) providing a three-dimensional scaffold housing a purified population of multi-lineage progenitor cells (MLPC) or a clonal line of MLPC; and culturing said purified population of MLPC or said clonal line of MLPC with a differentiation medium effective to induce differentiation of said MLPC into cells having said mature hepatocyte phenotype, wherein said MLPC within said purified population are positive for CD9, CD13, CD29, CD44, CD73, CD90 and CD105, and negative for CD10, CD34, CD41, CD45, Stro-1, SSEA-3, and SSEA-4, and wherein said mature hepatocyte phenotype comprises the presence of hepatocyte growth factor receptor and intracellular human serum albumin and the absence of intracellular pancreas-associated insulin and proinsulin production.