Patent ID: 6111066
Filing Date: 2000-08-29
Classification: C07B,C07K,C12N,C12P,G01R,Y10T

Abstract:
A substituted molecule comprising a peptidic sequence of 50 or more amino acids, wherein said molecule is isotopically substituted in the backbone structure but not the side chains of said amino acids or isotopically substituted in the hydrogen atoms bonded to the .alpha.-carbons of said amino acids or both and,wherein at least one species of amino acid in said peptidic sequence contains an isotopic substitution;wherein said isotopic substitution is selected from the group consisting of .sup.13 C; .sup.15 N; .sup.2 H; .sup.13 C and .sup.15 N; .sup.13 C and .sup.2 H; .sup.15 N and .sup.2 H; and .sup.13 C, .sup.15 N and .sup.2 H; andwherein said isotopic substitutions .sup.13 C; .sup.15 N; or .sup.13 C and .sup.15 N, when present, are present in substantially each occurrence of said at least one species of amino acid in said substituted molecule,with the proviso that when said species of amino acid is glycine, isotopic substitutions of .sup.2 H may occur on either or both of the hydrogen atoms bonded to the .alpha.-carbon.