Patent ID: 6849408
Filing Date: 2005-02-01
Classification: B01F,B01J,B01L,G01N,Y10S

Abstract:
1. A method for analyzing one or more fluid samples for the presence, amount or identity of one or more analytes in said samples, comprising the steps of: (a) providing a device comprising one or more round wells exposing a substrate, wherein the area of the substrate exposed in the well(s) is provided with at least one binding substance specific for at least one of said analytes; (b) adding an amount of sample fluid to one or more of the wells of the device; (c) generating an alternating flow through the substrate in the wells, whereby the liquid volume of the sample is forced to pass through the substrate from an upper side of the substrate to a lower side of the substrate and back at least one time, so as to allow for a reaction between analyte present in the sample and the binding substance(s); (d) reading any signal generated in any of the wells from the upper side of the substrate when the fluid has passed from the upper side of the substrate to the lower side of the substrate and is located as a drop underneath the substrate; and (e) determining from said signal(s) the presence, amount and/or identity of said one or more analytes.