Patent ID: 6242180
Filing Date: 2001-06-05
Classification: B01J,B82Y,C12Q,C40B,G01N,G16B

Abstract:
A computer implemented method of calling unknown bases in a sample nucleic acid sequence, comprising:inputting a plurality of probe intensities, each probe intensity indicating hybridization affinity between a nucleic acid probe and the sample nucleic acid sequence;calling bases of the sample nucleic acid sequence according to the plurality of probe intensities;identifying a mutant base call that has indicated that a base at a position in the sample nucleic acid sequence is suspected as being a mutation;analyzing probe intensities of at least one position that is near the position of the suspected mutation; andchanging the mutant base call to a nonmutant base call if the probe intensities of at least one position that is near the position of the suspected mutation are inconsistent with a mutation.