Patent ID: 8178504
Filing Date: 2012-05-15
Classification: A61K,A61P

Abstract:
1. A method of treating anemia in a mammalian subject, comprising: electroporating an effective amount of a nucleic acid expression construct that encodes a biologically active growth-hormone-releasing-hormone (“GHRH”) having SEQ ID NO: 6, into muscle cells of the mammalian subject, wherein the nucleic acid expression construct comprises a muscle specific synthetic promoter having sequence of SEQ ID NO: 7; and a nucleotide sequence encoding SEQ ID NO: 6, operatively linked to said synthetic promoter for functional expression in muscle tissue; wherein the expression construct causes an increase in GH synthesis or secretion in the mammalian subject when the expression construct is delivered into a muscle of the mammalian subject in combination with a transfection-facilitating polypeptide, thereby treating the anemia; wherein the anemia is caused by a decrease in red blood cells.