Patent ID: 6093537
Filing Date: 2000-07-25
Classification: C12Q,Y10S

Abstract:
A method for detecting the presence or absence of a target polynucleotide sequence in a sample which method comprises:a) combining in a medium said sample and a pair of nucleotide sequences, each nucleotide sequence of comprising a first and a second portion;b) hybridizing said first portion of the first nucleotide sequence with the target polynucleotide sequence and hybridizing said first portion of the second nucleotide sequence with the target polynucleotide sequence at a region different from that hybridized with the first nucleotide sequence;c) covalently or noncovalently attaching the hybridized first nucleotide sequence with the hybridized second nucleotide sequence to form an attached pair of nucleotide sequences;d) adding to said medium nucleotide sequence specific binding proteins (NSSBPs) that specifically bind the second portions of the attached pair of nucleotide sequences; ande) detecting the NSSBPs bound to the attached pair of nucleotide sequences, said detection indicating the presence of said target polynucleotide sequence.