Patent ID: 6576466
Filing Date: 2003-06-10
Classification: A61K,A61P

Abstract:
Activated lymphocytes obtained by a lymphocyte activing process, which have a killing, lysing or proliferation inhibiting activity in vitro towards each one of the tumor cells selected from the group consisting of MOLT-4 (ATTC CRL 1582), Jurkat (ATCC TIB 152), THP-1 (ATCC TIB 202), HL-60 (ATCC CCL 240), HeLa (ATCC CCL 2), K-562 (ATCC CCL 243), Malme-3M (ATCC HTB 64) and Y79 (ATCC HTB 18), wherein said activity is not MHC restricted and wherein said activated lymphocytes are T lymphocytes that bear a CD3 and at least one of CD4 or CD8 surface antigens and that proliferate in the presence of an activating agent without addition of a growth factor or a mitogen, which activating agent is,a) a mammalian activator cell line that is effective in generating activated lymphocytes from the donor lymphocytes, the cell line being DSM ACC 2122 (HB 654) or DSM ACC 2166 (HB 617); or b) a subcellular vesicle fraction of said activator cell line, wherein the fraction is effective in generating activated lymphocytes from the donor lymphocytes; or c) an extract of said cell line, wherein the extract is effective in generating activated lymphocytes from the donor lymphocytes, wherein said activated lymphocytes are obtained by a process wherein donor lymphocytes selected from the group consisting of blood lymphocytes, tumor infiltrating lymphocytes, spleen lymphocytes, and lymph node lymphocytes are incubated in a suitable lymphocyte culture medium in the presence of said activating agent, and without the addition of mitogens or growth factors, and wherein no interleukin 2 is detectable at a detection limit of 0.5 IU/ml in a supernatant obtained therefrom during proliferation of the cells, and wherein after said incubation said activated lymphocytes are present in said lymphocyte culture medium.