Patent ID: 8242257
Filing Date: 2012-08-14
Classification: A61K,A61P,C12N

Abstract:
1. A chemically modified double-stranded short interfering nucleic acid molecule comprising a sense strand and an antisense strand that mediates RNA interference (RNAi) against a target RNA sequence, wherein: a) each strand of the nucleic acid molecule is independently 18 to 24 nucleotides in length; b) the antisense strand is complementary to the target RNA sequence; c) the sense strand comprises 10 or more 2′-deoxy, 2′-O-methyl, 2′-deoxy-2′-fluoro, or universal base modified nucleotides, and all of the pyrimidine nucleotides present in the sense strand are 2′-deoxy, 2′-O-methyl, or 2′-deoxy-2′-fluoro pyrimidine nucleotides; and d) the antisense strand comprises 10 or more 2′-deoxy, 2′-O-methyl, 2′-deoxy-2′-fluoro, or universal base modified nucleotides, and all of the pyrimidine nucleotides present in the antisense strand are 2′-deoxy, 2′-O-methyl, or 2′-deoxy-2′-fluoro pyrimidine nucleotides.