Patent ID: 9145623
Filing Date: 2015-09-29
Classification: C12N,C12Q,C40B

Abstract:
1. An in vitro method for fragmenting target nucleic acids comprising contacting the target nucleic acids with a plurality of MuA transposon complexes, under a condition for transposon-mediated nucleic acid fragmentation, to generate a plurality of nucleic acid fragment products having a calibration sequence added to one end, wherein the MuA transposon complexes contain a first and a second nucleic acid duplex and a plurality of MuA transposon proteins, wherein the first and the second nucleic acid duplexes contain (i) a first strand having the nucleotide sequence of SEQ ID NO:1 that is modified to include a calibration sequence near the 3′ end, and (ii) a second strand that is fully complementary to the first strand, wherein the calibration sequence is four nucleotides in length and contains any nucleotide bases A, T, C, and/or G in any order and the calibration sequence replaces any four nucleotides in the first strand near the 3′ end in a region located at position 39-50 of SEQ ID NO:1.