Patent ID: 9012394
Filing Date: 2015-04-21
Classification: C12Q,G01N

Abstract:
1. A method for determining the amount of thyroglobulin in a test sample, comprising: (a) digesting thyroglobulin (Tg) and an added isotopically labeled thyroglobulin peptide standard in said test sample to form Tg peptides and Tg peptide standard products, wherein the Tg peptide standard has an amino acid sequence VIFDANAPVAVR (SEQ ID NO: (b) purifying said Tg peptides and Tg peptide standard products from step (a); (c) ionizing said Tg peptides and Tg peptide standard products from step (b) to produce one or more Tg peptide ions and Tg peptide standard product ions detectable by mass spectrometry; and (d) detecting the amount of the ion(s) from step (c) by mass spectrometry; wherein the amount of the ion(s) detected in step (d) is related to the amount of thyroglobulin in said test sample and the amount of thyroglobulin peptide standard that was digested in step (a).