Patent ID: 6482596
Filing Date: 2002-11-19
Classification: C07K,G01N

Abstract:
A method of measuring the association of two polypeptides comprising:obtaining a VH-domain polypeptide having a first reporter molecule attached; obtaining a VL-domain polypeptide having a second reporter molecule attached; mixing the two polypeptides with a compound that promotes association of the VH- and VL-domain polypeptides under conditions wherein the association of the VH-domain polypeptide with the VL-domain polypeptide is so weak in the absence of the compound that substantially no interaction between the first and second reporter molecules can occur without said compound; and measuring a characteristic value of the first reporter molecule or of the second reporter molecule which is induced by the interaction of the first and second reporter molecules upon association of the VH-domain polypeptide with the VL-domain polypeptide.