Patent ID: 7919267
Filing Date: 2011-04-05
Classification: C07K,C12N,C12Q,C12Y,G01N

Abstract:
1. A method for identifying a potential inhibitor of plasmidic class C β-lactamases having extended-substrate specificity, the method comprising the steps of: (a) providing a crystal of CMY-10 protein comprising the amino acid sequence set forth in SEQ ID NO: 2, the crystal having unit cell parameters of a=49.70 Å, b=59.51 Å, c=63.75 Å and β=102.57° and having its space group as P2 (b) determining a three-dimensional structure of said crystal; (c) designing or selecting a potential inhibitor of plasmidic class C β-lactamases by employing the three-dimensional structure; (d) synthesizing the potential inhibitor; and (e) contacting the potential inhibitor with CMY-10 protein in the presence of a substrate and measuring the ability of the potential inhibitor to inhibit CMY-10 protein.