Patent ID: 7883878
Filing Date: 2011-02-08
Classification: A23K,C12P

Abstract:
1. A method for preparing a dry granulated product containing L-lysine, comprising the steps of culturing a microorganism having an ability to produce L-lysine in a liquid medium under an aerobic condition to produce and accumulate L-lysine in the medium and obtaining the dry granulated product from the medium, wherein the culture is performed to obtain a fermentation solution containing L-lysine, while pH of the medium is controlled to be 6.5 to 9.0 during the culture and to be 7.2 to 9.0 at the end of the culture, and pressure in a fermentation tank is controlled to be positive during the culture to dissolve carbon dioxide into the medium, or carbon dioxide or a mixed gas containing carbon dioxide is supplied into the medium, so that there should be a culture period where bicarbonate and/or carbonate ions are present in the medium in an amount of 2 g/L or more, adding hydrochloric acid, sulfuric acid or an L-lysine solution having an equivalent ratio of anion/L-lysine higher than 0.95 to the fermentation solution so that the fermentation solution should have an equivalent ratio of anion/L-lysine in the range of 0.68 to 0.95, and the fermentation solution is concentrated under reduced pressure, and the obtained concentrate is granulated and dried to obtain a dry granulate product containing L-lysine and having the following composition: L-lysine content in solid matter: 40 to 85% by weight; equivalent ratio of anion/L-lysine: 0.68 to 0.95; moisture content: 5% by weight or less; wherein the equivalent ratio of anion/L-lysine is a value calculated in accordance with the following equation by using L-lysine (L-Lys) content, sulfate ion content, chloride ion content, ammonium ion content, sodium ion content, potassium ion content, magnesium ion content and calcium ion content in the solid matter of the dry granulated product: where [ ] means a molar concentration.