Patent ID: 8501468
Filing Date: 2013-08-06
Classification: C12M

Abstract:
1. A method of culturing cells in a compartmentalized roller bottle including a basal medium compartment having a first basal medium compartment end, a second basal medium compartment end and at least one basal medium compartment sidewall connecting said first basal medium compartment end and said second basal medium compartment end, at least one of which is comprised of a non-microporous semi-permeable membrane, a cell culture compartment residing within a roller bottle housing, an access port to said cell culture compartment, and an access port to said basal medium compartment, said roller bottle housing having a first end, a second end, and a sidewall connecting said first end to said second end, said cell culture compartment included in the volume of space within said roller bottle housing that is not occupied by said basal medium compartment, said cell culture compartment being bounded by at least a portion of said sidewall of said roller bottle housing, the method comprising a. adding a cell culture medium and adding cells into said cell culture compartment; b. adding a basal medium into said basal medium compartment; c. placing said compartmentalized roller bottle onto a roller rack and allowing said roller bottle housing to rotate upon said roller rack wherein at least a portion of said non-microporous semi-permeable membrane resides directly below at least a portion of said basal medium when said compartmentalized roller bottle is rolling; and d. said cell culture medium making at least periodic contact with said non-microporous semi-permeable membrane when said compartmentalized roller bottle is rolling.