Patent ID: 6413731
Filing Date: 2002-07-02
Classification: A01K,A61K,A61P,C07K,G01N

Abstract:
A method of screening a plurality of chemical compounds not known to bind to a human SNORF36a receptor to identify a compound which specifically binds to the human SNORF36a receptor, which comprises(a) contacting cells transfected with, and expressing, DNA encoding the human SNORF36a receptor with a compound known to bind specifically to the human SNORF36a receptor; (b) contacting the cells of step (a) with the plurality of compounds not known to bind specifically to the human SNORF36a receptor, under conditions permitting binding of compounds known to bind to the human SNORF36a receptor; (c) determining whether the binding of the compound known to bind to the human SNORF36a receptor is reduced in the presence of the plurality of compounds, relative to the binding of the compound in the absence of the plurality of compounds; and if so (d) separately determining the binding to the human SNORF36a receptor of each compound in the plurality of compounds, so as to thereby identify any compound which specifically binds to the human SNORF36a receptor; wherein the human SNORF36a receptor has an amino acid sequence identical to the amino acid sequence shown in SEQ ID NO:2 or that encoded by plasmid pcDNA3.1-hSNORF36a-f (ATCCÂ® Accession No. 203977).