Patent ID: 6750024
Filing Date: 2004-06-15
Classification: C07H,C09B

Abstract:
A method for detecting a target nucleic acid sequence comprising the steps of:providing a sample nucleic acid including at least one target nucleic acid sequence; and hybridizing a labeled oligonucleotide probe to the target nucleic acid sequence, the labeled oligonucleotide probe being labeled with an asymmetric cyanine dye compound having the structure: and including any associated counter ions, wherein:n ranges from 0 to 2; X is O, S or Se; R1 is selected from the group consisting of methyl and a linking group, or when taken together with the proximate carbon of the methine bridge forms a ring structure having from 5 to 7 members; R2 is a linking group; R3 is hydrogen, or when taken together with R4 forms a fused aromatic bridge substituted with one or more nitro groups; R4 is hydrogen, or when taken together with either R3 or R5 forms a fused aromatic bridge substituted with one or more nitro groups; R5 is nitro, or when taken together with either R4 or R6 forms a fused aromatic bridge substituted with one or more nitro groups; and R6 is hydrogen, or when taken together with either R5 forms a fused aromatic bridge substituted with one or more nitro groups.