Patent ID: 6448022
Filing Date: 2002-09-10
Classification: A61K,C07K,Y10S

Abstract:
A method for evaluating inhibition of human chorionic gonadotropin (hCG) production of a subject's trophoblast cells comprising the steps of isolating a regulatory polypeptidea) which inhibits, in a dose dependent manner, the production of hCG by human trophoblasts in vitro; b) which substantially inhibits the stimulation of hCG secretion by human trophoblasts caused by exogenous cyclic AMP in vitro; c) which does not substantially inhibit the production of human placental lactogen (hPL) by human trophoblasts in vitro; and d) which exhibits a molecular weight of about 7,000 to about 10,000 daltons, as determined by ultrafiltration and gel exclusion chromatography; from human decidual cells of said subject; and quantifying the amount of the regulatory polypeptide produced in vitro by said subject's human decidual cells.