Patent ID: 8628974
Filing Date: 2014-01-14
Classification: G01N

Abstract:
1. A method of identifying an amino acid residue of a polypeptide, the method comprising: ionizing a polypeptide reactant via matrix-assisted laser desorption/ionization or electrospray ionization; and then (a) performing a peptide degradation reaction on the polypeptide ion reactant in the gas phase, wherein the reaction yields a first ion product corresponding to a first amino acid residue of the polypeptide reactant and a polypeptide fragment ion; then (b) determining a mass-to-charge ratio for the first ion product, or the polypeptide fragment ion, or both; and then (c) identifying the first amino acid residue of the polypeptide reactant from the mass-to-charge ratio determined in step (b).