Patent ID: 6160203
Filing Date: 2000-12-12
Classification: C12N

Abstract:
A chimeric DNA that codes for an enzyme having glycerol-3-phosphate acyltransferase activity, the enzyme comprising a first partial amino acid sequence selected from the group consisting of position 126 (Lys) to 201 (Leu) of SEQ ID NO: 1, position 126 (Lys) to 201 (Leu) of SEQ ID NO: 2, position 126 (Lys) to 201 (Leu) of SEQ ID NO: 3, position 126 (Lys) to 201 (Leu) of SEQ ID NO: 4, position 126 (Lys) to 201 (Leu) of SEQ ID NO: 5, position 126 (Lys) to 201 (Leu) of SEQ ID NO: 6, position 126 (Lys) to 201 (Leu) of SEQ ID NO: 7, position 126 (Lys) to 201 (Leu) of SEQ ID NO: 8, position 126 (Lys) to 201 (Leu) of SEQ ID NO: 9, and position 126 (Lys) to 201 (Leu) of SEQ ID NO: 10, and a second partial amino acid sequence selected from the group consisting of position 245 (Trp) to 368 (Trp) of SEQ ID NO: 1, position 345 (Trp) to 368 (Trp) of SEQ ID NO: 2, position 245 (Trp) to 368 (Trp) of SEQ ID NO: 3, position 245 (Trp) to 368 (Trp) of SEQ ID NO: 4, position 245 (Trp) to 368 (Trp) of SEQ ID NO: 5, position 245(Trp) to 368 (Trp) of SEQ ID NO: 6, position 245 (Trp) to 368 (Trp) of SEQ ID NO: 7, position 245(Trp) to 368 (Trp) of SEQ ID NO: 8, position 245 (Trp) to 368 (Trp) of SEQ ID NO: 9, and position 245 (Trp) to 368 (Trp) of SEQ ID NO: 10, wherein the enzyme has a higher substrate selectivity for oleoyl-(acyl-carrier-protein) than for palmitoyl-(acyl-carrier-protein).