Patent ID: 6545006
Filing Date: 2003-04-08
Classification: B01J,C07D,G01N

Abstract:
A chromatographic process for separating L-dihydroorotic acid from a sample comprising the steps of:a) loading a sample solution comprising L-dihydroorotic acid and interfering material onto a high pressure liquid chromatography column containing a quantity of pressure-stable anionic exchange material effective to bind essentially all of said L-dihydroorotic acid in said sample; b) eluting said column with an aqueous basic eluting solution having a base concentration effective to remove said interfering material from said anionic exchange material without removing said L-dihydroorotic acid, until essentially all of said interfering material has been removed from said column; and (c) eluting said column with an aqueous basic eluting solution having a base concentration effective to remove said L-dihydroorotic acid from said anionic exchange material, until essentially all of said L-dihydroorotic acid has been removed from said column essentially free of said interfering material, wherein the operating pressure of said column is from about 1.1 MPa to about 40 MPa.