Patent ID: 7960508
Filing Date: 2011-06-14
Classification: C07K,G01N

Abstract:
1. A method for screening a substance for cytotoxicity-inhibitory activity, which method comprises: providing a cell culture; culturing the cells in the cell culture in serum free medium supplemented with albumin or a fatty acid, inducing cytoxicity in the cells by inducing apoptosis with the albumin or the fatty acid; contacting a test substance comprising a peptide with the cells in the cell culture; quantitating glutathione peroxidase (GPX) activity before and after contacting a test substance with the cells; and assessing an extent of cytotoxicity inhibition after contacting the test substance with the cells, wherein the GPX activity is inversely correlated with cytotoxicity, and wherein when the test substance causes an increase in the GPX activity in the cells compared to the GPX activity in the cells before contacting the cells with said test substance, the test substance is thereby identified as a cytotoxicity-inhibitory substance.