Patent ID: 8173442
Filing Date: 2012-05-08
Classification: G01N,Y10T

Abstract:
1. A method for determining the amount of 1α,25 dihydroxyvitamin D 2 (1α,25(OH) 2 D 2 ) in a biological sample, when taken from a human, by tandem mass spectrometry, comprising: (a) processing a biological sample from a human by one or more methods to generate a processed sample, wherein at least one method used is derivatizing 1α,25(OH) (b) enriching derivatized 1α,25(OH) (c) generating a precursor ion of the derivatized 1α,25(OH) (d) generating one or more fragment ions of said precursor ion, wherein at least one of said one or more fragment ions comprise an ion having a mass/charge ratio of 314.12±0.5; and (e) detecting the amount of one or more of said ions generated in step (c) or (d) or both by mass spectrometry and relating the detected ions to the amount of said 1α,25(OH)