Patent ID: 6306616
Filing Date: 2001-10-23
Classification: G01N,Y10S

Abstract:
An assay method for distinguishing between a small molecule analyte selected from the group consisting of LSD, LSD metabolites, and combinations thereof and an interfering substance in a sample comprising the steps of:a) preparing a reaction mixture comprising the sample, a hapten derivative that provides a signal and a detecting antibody that binds with the hapten derivative to yield a detectable signal in the assay, wherein the detecting antibody binds the hapten derivative in a manner that is specifically inhibitable by the analyte;b) measuring the amount of the detecting antibody bound to the hapten derivative in step a);c) treating the same sample or duplicate thereof with a neutralizing antibody in an amount sufficient to prevent the analyte but not all the interfering substance in the sample from binding the detecting antibody;d) preparing a reaction mixture comprising the treated sample or duplicate, a hapten derivative that provides a signal, and a detecting antibody that binds with the hapten derivative to yield a detectable signal in the assay, wherein the detecting antibody binds the hapten derivative in a manner that is specifically inhibitable by the analyte;e) measuring the amount of the detecting antibody bound to the hapten derivative in step d);f) determining the difference in signal measured in step e) from step b); andg) comparing the difference in signals in steps b) and e) to determine (i) the presence of analyte in the sample if the amount measured in step b) is significantly different from the amount measured in step e); or (ii) the presence of interfering substance in the sample if the amount measured in step b) is not significantly different from the amount measured in step e).