Patent ID: 9127312
Filing Date: 2015-09-08
Classification: C12Q

Abstract:
1. A method of detecting variations in copy number of a target nucleic acid, the method comprising: a. contacting a sample comprising a plurality of polynucleotides with at least one agent, wherein each of one or more of polynucleotides comprises multiple copies of a target nucleic acid; b. subjecting the sample to conditions that enable the agent to cleave a specifically-selected target site between the multiple copies of the target nucleic acid, thereby separating the multiple copies from one another; c. separating the sample contacted with the agent into a plurality of droplets; d. subjecting the target nucleic acid to an amplification reaction in droplets; e. detecting amplification data for the target nucleic acid from individual droplets; f. enumerating a number of droplets comprising the target nucleic acid based on the amplification data; and g. determining a copy number of the target nucleic acid based on the step of enumerating.