Patent ID: 7622300
Filing Date: 2009-11-24
Classification: C07K,C12N,Y10T

Abstract:
1. A transretroviral vector system comprising: a) at least a first polynucleotide sequence comprising at least one nucleotide sequence encoding a polypeptide selected from the group consisting of: wherein said first polynucleotide sequence does not encode a functional Gag polypeptide; and, b) a second polynucleotide sequence comprising a nucleotide sequence encoding at least a functional portion of a Gag polypeptide and a functional portion of a protease polypeptide, and said second polynucleotide sequence does not encode at least one of a functional Reverse Transcriptase polypeptide and a functional Integrase polypeptide and said second polypeptide is capable of expression in a mammalian cell; and, c) a third polynucleotide sequence comprising a nucleic acid sequence encoding a functional portion of an envelope polypeptide capable of mediating recognition and entry of the viral particle into a target cell, wherein said third polynucleotide sequence does not encode a functional Gag-Pol precursor polypeptide; d) a fourth polynucleotide sequence comprising a heterologous nucleic acid sequence of interest and at least one nucleotide sequence is selected from the group consisting of a functional equivalent of a polypurine tract-central terminator sequence (PPT-CTS), a functional equivalent of Woodchuck Hepatitis Virus Post transcriptional Regulatory Element (WPRE), a PPT-CTS, and a WPRE;