Patent ID: 6897041
Filing Date: 2005-05-24
Classification: A61K,A61P,C12N

Abstract:
1. A method of producing mature lysostaphin endopeptidase free from preprolysostaphin and prolysostaphin, the method comprising: (a) providing a genetic construct comprising a nucleotide sequence encoding mature lysostaphin endopeptidase comprising an initiation codon at its 5′-terminus in the absence of prolysostaphin-encoding elements, wherein the nucleotide sequence encoding the mature lysostaphin endopeptidase is operationally linked at its 5′ end to a promoter; (b) transforming a host cell to contain and express the genetic construct of step (a), such that mature lysostaphin, free from preprolysostaphin and prolysostaphin, accumulates in the cytoplasm of the host; and (c) isolating the mature lysostaphin from the host cell.