Patent ID: 6492111
Filing Date: 2002-12-10
Classification: A61K,C12N,C12Q,Y02A

Abstract:
A method for identifying cells containing a preselected nucleic acid sequence comprising:contacting said cells with a first oligonucleotide specifically bindable with said preselected nucleic acid sequence, said first oligonucleotide comprising a first identiphore; contacting said cells with a second oligonucleotide comprising a second identiphore, wherein said second oligonucleotide is specifically bindable with said preselected nucleic acid sequence at a site adjacent to said first oligonucleotide, causing said first and second identiphores to covalently bond with each other, wherein said covalent bonding gives rise to a signal detectable by spectroscopy, wherein said signal is absent in said first and second oligonucleotides prior to said covalent bonding; and identifying said cells by spectroscopic detection of said signal.