Patent ID: 6348569
Filing Date: 2002-02-19
Classification: A01K,A01N,C07K,C09K

Abstract:
An isolated or recombinantly expressed antifreeze protein, said protein comprising the following properties:(i) an apparent molecular weight of between about 5 and 20 kDa as determined by molecular exclusion chromatography; (ii) a thermal hysteresis activity of greater than about 1.5Â° C. at a concentration of about 1 mg/mL; and (iii) (a) specific binding to an antibody raised against an antifreeze protein selected from the group consisting of SEQ ID NO:2 and SEQ ID NO:3; and, (b) encoded by a nucleic acid which specifically hybridizes to a nucleic acid comprising a sequence as set forth in SEQ ID NO:1 under stringent conditions, wherein the stringent conditions include a wash step comprising a wash in 0.2Ã—SSC at a temperature of about 65Â° C. for about 15 minutes.