Patent ID: 9192943
Filing Date: 2015-11-24
Classification: B01L,B03C,C12M,F16K,G01N

Abstract:
1. A microfluidic device for isolation of particles (C 1 ) of at least one given type from a sample; the device ( 73 ) being designed to be connected to an apparatus ( 72 ); the microfluidic device ( 73 ) comprising: electrical connectors for connecting the device ( 73 ) itself to the apparatus; a first inlet ( 2 ), through which, in use, the sample is introduced into the microfluidic device ( 73 ); a separation unit ( 3 ), which is connected to the first inlet ( 2 ), comprises a main chamber ( 4 ) and a recovery chamber ( 5 ), and is designed to transfer at least part of the particles (C 1 ) of the given type from the main chamber ( 4 ) to the recovery chamber ( 5 ) in a substantially selective way with respect to further particles (C 2 ) of the sample, wherein the main chamber is separated from the recovery chamber by at least one chamber wall and the main chamber is fluidly coupled and connected to the recovery chamber by a channel; a first outlet ( 7 ), connected to the main chamber ( 4 ); a second outlet ( 8 ), which is connected to the recovery chamber ( 5 ), and through which, in use, at least part of the particles (C 1 ) of the given type collected in the recovery chamber ( 5 ) exit from the microfluidic device ( 73 ); a first valve portion ( 74 ) designed to form part of a first valve ( 9 ), said first valve portion ( 74 ) being set upstream of the main chamber ( 4 ) between the first inlet ( 2 ) and the main chamber ( 4 ); a second valve portion ( 75 ), designed to form part of a second valve ( 10 ), said second valve portion ( 75 ) being set between the main chamber ( 4 ) and the first outlet ( 7 ); a third valve portion ( 76 ), designed to form part of a third valve ( 11 ), said third valve portion ( 76 ) being connected to the recovery chamber ( 5 ); and a fourth valve portion ( 77 ), designed to form part of a fourth valve ( 12 ), said fourth valve portion ( 77 ) being set between the recovery chamber ( 5 ) and the second outlet ( 8 ); wherein: the recovery chamber ( 5 ) is set between the main chamber ( 4 ) on one side and the third and fourth valve portions ( 76 , 77 ) on the other side; the main chamber (