Patent ID: 6130367
Filing Date: 2000-10-10
Classification: C12N

Abstract:
An isolated DNA molecule encoding a protein with the biological activity of a starch synthase or activity for modifying starch metabolism in a plant or plant cell, said DNA molecule selected from the group consisting of:(a) a DNA molecule encoding a protein having the amino acid sequence of SEQ ID NO. 8;(b) a DNA molecule comprising the nucleotide sequence of SEQ ID NO. 7;(c) a DNA molecule comprising a nucleotide sequence differing from the sequence of the DNA molecules of (a) or (b) due to the degeneracy of the genetic code;(d) a DNA molecule comprising a DNA sequence which hybridizes under stringent conditions to any one of the DNA molecules of (a), (b) or (c) or fragment thereof, and which is more than 90% homologous to the DNA molecule of (a), (b), or (c) or fragment thereof, wherein said DNA sequence encodes a protein with the biological activity of a starch synthase of isotype II (GBSSII) or a biologically active fragment thereof, or encodes a protein with activity for modifying starch metabolism in a plant or plant cell;(e) a DNA molecule encoding a protein having the amino acid sequence of SEQ ID NO: 10;(f) a DNA molecule comprising the nucleotide sequence of SEQ ID NO: 9;(g) a DNA molecule comprising a nucleotide sequence differing from the sequence of any one of the DNA molecules of (e) or (f) due to the degeneracy of the genetic code;(h) a DNA molecule comprising a DNA sequence which hybridizes under stringent conditions to any one of the DNA molecules of (e), (f) or (g) or fragment thereof, and which is more than 90% homologous to the DNA molecule of (e), (f), or (g) or fragment thereof, wherein said DNA sequence encodes a protein with the biological activity of a soluble starch synthase of isotype B (SSSB) or a biologically active fragment thereof, or encodes a protein with activity for modifying starch metabolism in a plant or plant cell;(i) a DNA molecule encoding a protein having the amino acid sequence of SEQ ID NO: 12;(k) a DNA molecule comprising the nucleotide sequence of SEQ ID NO: 11;(l) a DNA molecule comprising a nucleotide sequence differing from the sequence of the DNA molecules of (i) or (k) due to the degeneracy of the genetic code; and(m) a DNA molecule comprising a DNA sequence which hybridizes under stringent conditions to any one of the DNA molecules of (i), (k) or (l) or fragment thereof, and which is more than 90% homologous to the DNA molecule of (i), (k), or (l) or fragment thereof, wherein said DNA sequence encodes a protein with the biological activity of a soluble starch synthase of isotype A (SSSA) or a biologically active fragment thereof, or encodes a protein with activity for modifying starch metabolism in a plant or plant cell.