Patent ID: 7575904
Filing Date: 2009-08-18
Classification: A61K,A61L,C12N,C12P,Y10S

Abstract:
1. A method for elongating a polysaccharide to produce a polysaccharide polymer on a substrate, comprising the steps of: providing a substrate having an immobilized polysaccharide primer thereon to provide a primed substrate, wherein the primed substrate comprises at least two sugar units selected from the group consisting of GIcUA, GIcNAc, GIc, GaINAc, GIcN, and GaIN; combining the primed substrate within a reaction medium with an isolated, enzymatically active hyaluronan synthase having an empty acceptor site, wherein the isolated, enzymatically active hyaluronan synthase is a single protein that is a dual-action catalyst that utilizes UDP-GIcA and UDP-GIcNAc to synthesize hyaluronic acid (HA) on a substrate, wherein the reaction medium contains at least one sugar precursor selected from the group consisting of UDP-GIcA and UDP-GIcNAc, and wherein the enzymatically active hyaluronan synthase has an amino acid sequence that is at least one of: (A) an amino acid sequence that is at least 90% identical to SEQ ID NO: 1; (B) an amino acid sequence encoded by a nucleic acid segment that is at least 90% identical to SEQ ID NO: 2; and (C) an amino acid sequence selected from the group consisting of SEQ ID NOS: 7, 8, 11 and 13-17; and reacting the isolated, enzymatically active hyaluronan synthase with the primed substrate to produce a polysaccharide polymer coating on the primed substrate.