Patent ID: 8815576
Filing Date: 2014-08-26
Classification: B01L,C12Q

Abstract:
1. An apparatus for sequencing nucleic acids in a sample, wherein the sample contains the nucleic acids; comprising: a microchip; a flow channel in said microchip; a source of emulsion carrier fluid connected to said flow channel producing an emulsion carrier fluid in said flow channel; a sample and fluid source that provides a source of the sample that contains the nucleic acids and a fluid; a sample and fluid channel connected to said sample and fluid source; a source of magnetic particles; a magnetic particles channel connected to said source of magnetic particles; a sample, fluid, and magnetic particles channel connected to said sample and fluid channel and to said magnetic particles channel; said sample, fluid, and magnetic particles channel connecting the sample containing the nucleic acids, said fluid, and said magnetic particles to said flow channel; an emulsion microreactor maker connected to said flow channel for producing microreactors containing the sample containing nucleic acids, said fluid, and said magnetic particles, said emulsion microreactor maker including said emulsion carrier fluid, the sample, said fluid, said magnetic particles, and a junction in said flow channel, wherein the sample, said fluid, and said magnetic particles are injected into said emulsion carrier fluid in said flow channel producing said microreactors containing the sample, said fluid, and said magnetic particles suspended in said emulsion carrier fluid; a reagent source connected to said flow channel; a nucleotides source connected to said flow channel for introducing NTP a PCR and sequencing zone in said flow channel; an electromagnet trap for selectively magnetically trapping the nucleic acids and said magnetic particles at said PCR and sequencing zone in said flow channel; thermalcycler connected to said PCR and sequencing zone in said flow channel; and a detector for detection and sequencing of the nucleic acids.