Patent ID: 6200813
Filing Date: 2001-03-13
Classification: C07H,C12N,C12P

Abstract:
A method for altering the substrate specificity of a polykefide synthase in a first polyketide-producing microorganism comprising the steps of:(a) isolating a first and second genomic DNA segment, each comprising a polyketide synthase wherein said first genomic DNA segment is from said first polyketide-producing microorganism and said second genomic DNA segment is from said first polyketide-producing microorganism or a second polyketide-producing microorganisin;(b) identifying one or more discrete fragments of said first genomic DNA segment, each of which encodes an acyltransferase domain;(c) identifying one or more discrete fragments of said second genomic DNA segment, each of which encodes a related domain to said acyltransferase domain of said first genomic DNA segment; and(d) transforming a cell of said first polyketide-producing microorganism with one or more of said fragments from step (c) under conditions suitable for the occurrence of a homologous recombination event, leading to the replacement of one or more of said fragments from said first genomic DNA segment with one or more of said fragments from step (c).