Patent ID: 8076114
Filing Date: 2011-12-13
Classification: C12N,C12P,Y02E,Y02P

Abstract:
1. An isolated polypeptide having activity of a cytochrome P450 variant, wherein the variant is characterized as comprising a higher capability than the corresponding wild-type cytochrome P450 to oxidize at least one substrate selected from an alkane comprising a carbon-chain of no more than 8 carbons and an alkene comprising a carbon-chain no more than 8 carbons, and wherein the wild-type cytochrome P450 comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO: 2, and the cytochrome P450 variant comprises an amino acid substitution at two residue of SEQ ID NO:2 selected from the group consisting of V78, F87, T235, R471, E494 and S1024.