Patent ID: 6562575
Filing Date: 2003-05-13
Classification: C12Q

Abstract:
A method for assaying for an analyte in a sample comprising the steps of:(a) incubating a sample with a reporter probe under conditions and for a time so as to permit binding of said reporter probe with analyte, if present in sample, said reporter probe comprising a first portion, wherein said first portion is a polynucleotide that encodes at least part of a sequence for a replicase substrate, and a second portion, wherein said second portion has affinity for the analyte under binding conditions, and wherein said reporter probe is not a substrate for replication by said replicase; and (b) incubating said reporter probe which binds to analyte with a composition having nuclease activity, wherein said composition releases all parts of said first portion of said reporter probe from said second portion of said reporter probe; and (c) incubating said released parts of said first portion of said reporter probe with a composition having ligase activity so as to form a polynucleotide which encodes a complete substrate for replication by a replicase, and providing conditions so as to generate said substrate; and (d) incubating said substrate with a replicase under replication conditions; and (e) detecting the products of said replication.