Patent ID: 6103528
Filing Date: 2000-08-15
Classification: C12N

Abstract:
A gelling cell culture medium, useful for forming a three dimensional matrix for cell culture in vitro, the gelling cell culture medium, comprising:(a) a reversible gelling copolymer that is a linear random copolymer of:(i) a monomer selected from the group consisting of an acrylamide derivative, a methacrylamide derivative and combinations thereof; and(ii) a hydrophilic comonomer different from said monomer in (i);said linear random copolymer in the form of a plurality of linear chains having a plurality of molecular weights greater than or equal to a minimum gelling molecular weight cutoff;(b) an aqueous solvent mixed with said reversible gelling copolymer as a reversible gelling solution; and(c) a cell culture medium mixed with said reversible gelling solution as said gelling cell culture medium.