Patent ID: 8541229
Filing Date: 2013-09-24
Classification: C12N

Abstract:
1. A lambda phage comprising, a promoter operably linked to a nucleic acid sequence encoding a heterologous nucleic acid sequence; P a phage origin of replication, and a nucleic acid sequence encoding cI857; wherein the nucleic acid sequence encoding Cro comprises an amber codon, the nucleic acid sequence encoding P, the nucleic acid sequence encoding O, or both the nucleic acid sequence encoding P and the nucleic acid sequence encoding O comprise an amber codon, such that Cro and P, Cro and O, or Cro, P and O are not produced when the lambda phage is introduced into a suppressor minus host cell; wherein the lambda phage does not comprise a bacterial origin of replication; and wherein the lambda phage is lysogenic in a suppressor minus host cell and lytic at 42° C. in a host cell that produces an amber suppressive tRNA.