Patent ID: 7964371
Filing Date: 2011-06-21
Classification: G01N

Abstract:
1. A method for determining the total amount of gastrin hormone in a biological fluid sample, comprising the steps of: (a) obtaining a biological fluid sample comprising gastrin hormone from a patient; (b) providing an immobilized antibody that selectively binds a C-terminal epitope of the gastrin hormone; (c) incubating the sample in the presence of an N-terminal sequence gastrin peptide under suitable conditions for binding of the gastrin hormone in the sample to said antibody to produce an immobilized complex of said antibody bound to the gastrin hormone; (d) washing the immobilized complex to remove N-terminal sequence gastrin peptide, and incubating the complex with a suitable detectable marker-conjugated antibody that selectively binds an N-terminal epitope of gastrin hormone to form an immobilized detectable marker-conjugated antibody complex; (e) washing the immobilized detectable marker-conjugated antibody complex, and incubating with a development reagent; and (f) measuring the developed reagent to determine the total amount of the gastrin hormone in the biological fluid sample; wherein the immobilized antibody that selectively binds a C-terminal epitope of the gastrin hormone is a monoclonal antibody selected from the monoclonal antibody produced by hybridoma 458-1 (ATCC accession no. PTA-5896), the monoclonal antibody produced by hybridoma 445-1 (ATCC accession no. PTA-5894) and the monoclonal antibody produced by hybridoma 445-2 (ATCC accession no. PTA-5895).