Patent ID: 7653490
Filing Date: 2010-01-26
Classification: G01N

Abstract:
1. A method for obtaining a binding compound having specificity for a member of an enzyme family, wherein the members of the enzyme family bind a common ligand, comprising the steps of: (a) observing competitive binding of the common ligand and a first ligand to a first enzyme; (b) observing competitive binding of the common ligand and a first ligand to a second enzyme, wherein the first and second enzymes are members of the enzyme family, thereby determining that the first ligand binds to a common ligand binding site of the first and second enzymes; (c) providing a sample comprising the first enzyme, the first ligand and a second ligand; (d) providing a sample comprising the second enzyme, the first ligand and the second ligand; (e) determining the degree of magnetization transfer between the first ligand and the second ligand for the samples of parts (c) and (d); (f) comparing the degree of magnetization transfer between the first ligand and the second ligand for the samples of parts (c) and (d), whereby comparing the degree of magnetization transfer identifies a second ligand that selectively binds to the first or second enzyme; and (g) generating a binding compound by linking the first ligand, or a binding fragment thereof, to the second ligand or a binding fragment thereof via a linker, said linker joining proximal portions of said first and said second ligands; thereby obtaining a binding compound having specificity for the first enzyme, wherein said second ligand selectively binds to the first enzyme, or having specificity for the second enzyme, wherein said second ligand selectively binds to the second enzyme.