Patent ID: 6686172
Filing Date: 2004-02-03
Classification: C12Q,G01N,Y10S

Abstract:
A method for detecting or determining homocysteine in a sample, comprising:(a) reducing the homocysteine in the sample with a thiol compound, (b) reacting the reduced homocysteine with a homocysteine-converting enzyme and a homocysteine cosubstrate, thereby producing a homocysteine converting enzyme product, and (c) detecting or determining the residual homocysteine cosubstrate, the produced homocysteine-converting enzyme product or an enzyme reaction product thereof by (i) oxidizing the residual homocysteine cosubstrate, the produced homocysteine-converting enzyme product or an enzyme reaction product thereof in the presence of an SH reagent to produce hydrogen peroxide and detecting or determining the produced hydrogen peroxide by color development using an oxidative color-developing agent or (ii) reacting the residual homocysteine cosubstrate, the produced homocysteine-converting enzyme product or an enzyme reaction product thereof with a D-amino acid converting enzyme to produce an oxo acid and/or ammonia and detecting or determining the produced oxo acid and/or ammonia.