Patent ID: 6153394
Filing Date: 2000-11-28
Classification: G01N

Abstract:
In a method for the detection of Sarcocystis neurona in an immunoassay where a protein of the Sarcocystis neurona is reacted with antibodies from an equine suspected of harboring the Sarcocystis neurona to form a complex which is detected with a labeled antibody that reacts with the antibodies in the complex, the labeled antibody contains a label which is then detected, wherein the improvement comprises:reacting the protein of the Sarcocystis neurona with a non-labeled antibody to proteins of a Sarcocystis sp. other than the Sarcocystis neurona from a species of mammal other than equine prior to reaction with the equine antibodies so that non-specific binding of the equine antibodies is inhibited to thereby detect the Sarcocystis neurona.