Patent ID: 6603869
Filing Date: 2003-08-05
Classification: G06K,G06T

Abstract:
In an image analysis apparatus, a method of analyzing a plurality of cell nuclei and truncated cell nuclei in a tissue section, said method comprising:a) measuring by said image analysis apparatus the DNA content, profile area, and profile perimeter of each of said plurality of cell nuclei and truncated cell nuclei, and (b) using said profile perimeter to calculate an eccentricity index for each one of said plurality of cell nuclei and truncated cell nuclei, and (c) applying a ploidy method for analyzing quantitative nuclear DNA content in a tissue section wherein said ploidy method utilizes said eccentricity indicies, whereby the discrimination of subpopulations of different ploidy among the whole nuclei corresponding to said plurality of cell nuclei and truncated cell nuclei is improved, and the accuracy of the determination of the true DNA content of said whole nuclei is improved.