Patent ID: 7666658
Filing Date: 2010-02-23
Classification: C07B,C07D,C12N,C12P

Abstract:
1. A method to improve kinetic resolution of β-hydroxyketones from a racemic mixture by means of a retro-aldol reaction, wherein the method comprises catalyzing said retro-aldol reaction with antibody molecules produced according to the following method: Step A: eliciting an immune response within a subject by vaccination with a sterile solution containing the appropriate concentration of an aldol transition state immunoconjugate, said aldol transition state immunoconjugate comprising a sulfone β-diketone hapten, thus producing a catalytic aldolase antibody; then Step B: isolating and cloning an antibody producing cell from the subject of said Step A which expresses the catalytic aldolase antibody; and then Step C: isolating the catalytic aldolase antibody expressed by the antibody producing cell isolated and cloned in said Step B; wherein the aldol transition state immunoconjugate of said Step A comprises the following structure: where n is greater than or equal to 2 and less than or equal to 8, and wherein the racemic mixture is treated with a catalytic amount of the antibody in an aqueous buffer to give a simple ketone, an aldehyde containing a phenyl ring and an enantiomerically enriched β-hydroxyketone.