Patent ID: 9110065
Filing Date: 2015-08-18
Classification: C07K,C12Q,G01N

Abstract:
1. A method for diagnosing aggressive thyroid cancer in a subject, the method comprising: (A) determining a nuclear Ep-ICD score, a cytoplasmic Ep-ICD score and a membrane EpEx score in a thyroid tissue sample obtained from the subject, the thyroid tissue comprising cells with each cell having a nucleus, cytoplasm and a cell membrane, the step of determining comprising: (B) calculating an Ep-ICD Subcellular Localization Index (ESLI) value for the sample, the ESLI value being a sum of the nuclear Ep-ICD score, the cytoplasmic Ep-ICD score and a membrane EpEx loss value, wherein the membrane EpEX loss value is determined by subtracting the calculated membrane EpEX score from a theoretical maximum of the membrane EpEX score; (C) comparing the calculated ESLI value to a reference value, wherein the control reference value is: (D) diagnosing aggressive thyroid cancer in the subject when the calculated ESLI value of the sample is greater than the reference value of (C)(i) or is greater than or equal to the reference value of (C)(ii); wherein: the first, second and third percentages are obtained using immunohistochemical (IHC) analysis; the first, second and third intensities are determined using immunohistochemical (IHC) analysis; and wherein at least the first binding agent is a rabbit monoclonal antibody.