Patent ID: 8206970
Filing Date: 2012-06-26
Classification: C12N,C12P,Y02E

Abstract:
1. A recombinant microbial host cell comprising at least one heterologous DNA molecule encoding a polypeptide that catalyzes a substrate to product conversion of 3-amino-2-butanol phosphate to 2-butanone and at least one heteroloqous DNA molecule encoding a polypeptide that catalyzes a substrate to product conversion of 3-amino-2-butanol to 3-amino-2-butanol phosphate; wherein said microbial host cell produces 2-butanol by the conversion of 3-amino-2butanol phosphate to 2-butanone; and wherein the polypeptide that catalyzes a substrate to product conversion of 3-amino-2-butanol phosphate to 2-butanone is aminobutanol phosphate phospho-lyase having the amino acid sequence that has at least 95% identity to the amino acid sequence of SEQ ID NO:126 based on the Clustal W method of alignment using the default parameters of GAP PENALTY=10, GAP LENGTH PENALTY=0.1, and Gonnet 250 series of protein weight matrix.