Patent ID: 8879060
Filing Date: 2014-11-04
Classification: G01N

Abstract:
1. A method of determining the content of an analyte in a sample comprising the steps of: a. generating an optical signal by emitting at least one excitation light to said sample; b. obtaining a modulated Raman signal comprising a first component and a second component by: wherein said first periodic function and said second periodic function have same predefined period and are orthogonal to each other; c. computing the intensities of said first component and said second component based on the first harmonic of said modulated Raman Signal, wherein said step of computing said intensities of said first component and said second component further comprises a step of extracting an in-phase magnitude and a quad-phase magnitude of said first harmonic of said modulated Raman signal based on said predefined period; wherein said in-phase magnitude corresponds to said intensity of said first component and said quad-phase magnitude corresponds to said intensity of said second component; and d. determining said content of said analyte based on a ratio of said intensities of said first component and said second component.