Patent ID: 8334139
Filing Date: 2012-12-18
Classification: C12N

Abstract:
1. A co-integrate Ti plasmid for use in transforming plant cells comprising a T-DNA region having the following elements, in sequence: (a) a first T-DNA border sequence; (b) a plant gene comprising a 5′ promoter region from a gene which exists naturally in a plant cell or naturally enters a plant cell, a structural coding sequence encoding a polypeptide expressable in plant cells and a 3′ non-translated region encoding a polyadenylation signal, said 5′ promoter region and 3′ non-translated region being operably linked to said structural coding sequence; and (c) a second T-DNA border sequence, wherein said T-DNA border sequences enable the transfer and incorporation of T-DNA into the genome of a plant cell and there are no plant tumorigenic genes between and including the two T-DNA border sequences which would render a transformed plant cell tumorous or incapable of regeneration into a morphologically normal plant.