Patent ID: 7388092
Filing Date: 2008-06-17
Classification: C07H,C09B,C12Q,G01N

Abstract:
1. A fluorescently labeled reagent comprising an oligonucleotide or oligonuclotide analog and an energy transfer dye, wherein energy transfer dye is covalently linked to the oligonucleotide or oligonucleotide analog and comprises: a xanthene donor dye capable of absorbing light at a first wavelength and emitting excitation energy in response thereto; a 4,7-dichlororhodamine acceptor dye capable of absorbing the excitation energy emitted by the donor dye and fluorescing at a second wavelength in response thereto; and a non-nucleosidic linker linking the xanthene donor dye to the 4′-, 5- or 6-position of the 4,7-dichlororhodamine acceptor dye.