Patent ID: 6824975
Filing Date: 2004-11-30
Classification: G01N,Y10S

Abstract:
A lateral flow immunoassay device for detecting antibodies to a specific analyte, said device comprising:a test strip for detecting IgG, IgA and IgM in a sample, said test strip comprising: i) a sample site for applying said sample, said sample site comprising: a) a sample pad to which said sample is applied; and b) an IgG reacting substance present at said sample site, said substance binds to any IgG present in said sample thereby slowing down the migration of said IgG and allowing other antibodies in said sample to migrate and react at sites downstream from said sample pad prior to the migration of said IgG; ii) a colorimetric labeling site positioned downstream from said sample site, said colorimetric labeling site comprising colorimetric labeled binding partners for said IgG, IgA and IgM; and iii) at least four reaction sites positioned downstream from said colorimetric labeling site, said reaction sites comprising immobilized binding partners for said IgG, IgA, and IgM, and a control reaction site; wherein complexes comprising colorimetric labeled binding partner-sample antibody-immobilized binding partner are formed when said antibodies are present in said sample, and a colored line will appear at each of said reaction sites indicating the present of said complexes.