Patent ID: 8367417
Filing Date: 2013-02-05
Classification: C12Q,Y10T

Abstract:
1. A method for determining predisposition to or diagnosis of autism spectrum disorder in an individual, comprising: isolating DNA from cells of an individual; digesting the DNA; performing polymerase chain reaction using primers that flank a Hpall site in the first exon of and flanking sequence of each gene of a set of genes of the individual, wherein the set of genes comprises NLGN4, NLGN3, L1 CAM, and AVPR2; determining the cytosine methylation level of the genes; comparing the cytosine methylation level of the genes to a control cytosine methylation level of corresponding genes; and finding that at least three of NLGN4, NLGN3, L1CAM and AVPR2 of the individual are hypermethylated or hypomethylated as compared to the control cytosine methylation level of the corresponding genes to determine that the individual is predisposed to or affected with autism spectrum disorder.