Patent ID: 6265176
Filing Date: 2001-07-24
Classification: G01N,Y10S

Abstract:
A test kit for detecting the presence in a sample of at least one component to be detected selected from the group consisting of antigens, antibodies and hastens by a sandwich enzyme immunoassay comprising in one or more containers:a. an analytical device consisting essentially of an inert, opaque plastic carrier having a flat, hydrophobic surface that is non-absorbent to said antigens, antibodies, hastens and to enzymes, having a multiplicity of visually locatable discrete locations and, deposited directly on said surface within at least one of said discrete locations, a spot of an insolubilized specific first binding partner of the at least one component to be detected;b. a working reagent conjugate of predetermined concentration of at least about 15 micrograms per ml in liquid form comprising an enzyme coupled to a specific second binding partner of the at least one component to be detected; andc. an enzyme substrate in liquid form comprising a material capable of reacting with said enzyme at room temperature to form a colored precipitate detectable by the naked eye against said opaque carrier.