Patent ID: 6673347
Filing Date: 2004-01-06
Classification: A61K,C07K,G01N

Abstract:
A composition containing conjugated derivatized protein or polypeptide molecules, or salts thereof, wherein said conjugated derivatized protein or polypeptide molecules of said composition consist essentially of a compound selected from the formula:Aâ€”Xâ€”Zâ€”Xâ€²â€”Bwherein:A is a protein or polypeptide, and wherein A has a carboxy and amino terminus and is conjugated to Xâ€”Zâ€”Xâ€²â€”B only at either said carboxy terminus of A or at said amino terminus of A; B is a polymeric compound; X and Xâ€² independently from each other are bivalent organic radicals or independently from each other are present or absent; Z is a bivalent radical selected from the group consisting of: â€”C(R)&boxH;Nâ€”, â€”N&boxH;C(R)â€”, â€”CH(R)â€”NHâ€”, â€”NHâ€”CH(R)â€”, â€”C(R)&boxH;Nâ€”Yâ€”N&boxH;C(R)â€”, â€”N&boxH;C(R)â€”Yâ€”C(R)&boxH;Nâ€”, â€”CH(R)â€”NHâ€”Yâ€”NHâ€”CH(R)â€”, â€”NHâ€”CH(R)â€”Yâ€”CH(R)â€”NHâ€”, â€”C(R)&boxH;Nâ€”Oâ€”, â€”Oâ€”N&boxH;C(R)â€”, â€”CR(R)â€”NHâ€”Oâ€”, â€”Oâ€”NHâ€”CH(R)â€”, â€”C(R)&boxH;Nâ€”Oâ€”Yâ€”Oâ€”N&boxH;C(R)â€”, â€”Oâ€”N&boxH;C(R)â€”Yâ€”C(R)&boxH;Nâ€”Oâ€”, â€”CH(R)â€”NHâ€”Oâ€”Yâ€”Oâ€”NHâ€”CH(R)â€” and â€”Oâ€”NHâ€”CH(R)â€”Yâ€”CH(R)â€”NHâ€”Oâ€”; where R is hydrogen or an aliphatic, cycloaliphatic, aromatic or araliphatic hydrocarbon group and Y is a bivalent organic group; wherein said conjugated derivatized protein or polypeptide molecules of said composition are produced via formation of a Schiff base, hydrazone, oxime or azomethine compound that results in the presence of at least one radical having the formula â€”C(R)&boxH;Nâ€”, or â€”N&boxH;C(R)â€”, or, when reduced, â€”CH(R)â€”NHâ€” or â€”NHâ€”CH(R)â€”.