Patent ID: 7205129
Filing Date: 2007-04-17
Classification: C12Q

Abstract:
1. A method of reducing formation of artifacts in a nucleic acid amplification reaction, the method comprising conducting a nucleic acid amplification reaction using a template-deficient oligonucleotide as a primer and using a polymerase, wherein the template-deficient oligonucleotide comprises one or more template-deficient nucleotides, wherein the number and composition of template-capable nucleotides 3′ of the template-deficient nucleotide closest to the 3′ end of the template-deficient oligonucleotide is sufficient to allow the template-capable nucleotides 3′ of the template-deficient nucleotide closest to the 3′ end alone to effectively prime nucleic acid synthesis in the nucleic acid amplification reaction reaction, wherein the template-deficient oligonucleotide is template-deficient when used in a nucleic acid amplification reaction with the polymerase.