Patent ID: 6048707
Filing Date: 2000-04-11
Classification: C07H,G01N

Abstract:
A method of analyzing structural relationships between monosaccharides of a carbohydrate comprising:a) derivatizing a carbohydrate with a carbohydrate derivatizing agent which covalently binds to the free functional groups of the carbohydrate to form a derivatized carbohydrate;b) converting said derivatized carbohydrate into derivatized monosaccharides by breaking the glycosidic bonds of said derivatized carbohydrate;c) forming labeled derivatized monosaccharides by reacting said derivatized monosaccharides with a fluorescence labelling compound;d) separating said labeled derivatized monosaccharides by electrophoresis; ande) identifying said labeled derivatized monosaccharides by comparing the electrophoresis mobility of said labeled derivatized monosaccharides to a monosaccharide standard, said monosaccharide standard comprising monosaccharides derivatized with said carbohydrate derivatizing agent and labelled with said fluorescence labelling compound.