Patent ID: 8679806
Filing Date: 2014-03-25
Classification: C07K,C12Q,G01N

Abstract:
1. A method for determining whether or not a solution includes specific bacterial molecules, the method comprising: a) providing a reference electrode in the solution; b) providing a working electrode in the solution, the working electrode including an electrode substrate having a layer of polymer applied thereto, and bacterial specific selector molecules, wherein the working electrode is constructed by exposing the electrode substrate to a suitable monomer in the presence of the bacterial specific selector molecules such that when the monomer is polymerized, the selector molecules are substantially immobilized thereto, and wherein the working electrode is constructed in accordance with an optimal concentration of the bacterial specific selector molecules and an optimal immersion time that are predetermined to produce a peak potential response during a testing, the optimal concentration being approximately 0.3 mg/ml and the optimal immersion time being approximately 4 minutes, and wherein the layer of polymer is a monolayer of Polysiloxane, and wherein if the selector molecules are exposed to the specific bacterial molecules, a change in electrical potential is caused; c) determining a potential difference between the reference electrode and the working electrode; and d) determining whether or not the solution includes the specific bacterial molecules using the determined potential difference.