Patent ID: 8163888
Filing Date: 2012-04-24
Classification: A61K,A61P,C07K,Y02A

Abstract:
1. A process of making a cell-binding agent maytansinoid conjugate, the process comprising: (a) modifying a thiol-containing maytansinoid with a cross-linking agent to thereby form a non-cleavable linker comprising a S atom, and (b) conjugating the modified thiol-containing maytansinoid with the cell-binding agent, thereby providing the non-cleavable linker between the cell-binding agent and the thiol-containing maytansinoid to produce a conjugate, wherein the non-cleavable linker is a linker that is substantially resistant to acid-induced cleavage, light-induced cleavage, peptidase-induced cleavage, esterase-induced cleavage, and disulfide bond cleavage, and wherein said cell-binding agent is an antibody, a single chain antibody or an antibody fragment that specifically bind to a target cell, wherein the antibody, single chain antibody or antibody fragment comprises a human constant region.