Patent ID: 6242233
Filing Date: 2001-06-05
Classification: C12N,C12P

Abstract:
A purified aldehyde dehydrogenase, wherein the dehydrogenase has a molecular weight of 150,000.+-.6,000 Da and comprises two homologous subunits or has a molecular weight of 230,000.+-.9,000 Da and comprises three homologous subunits, each subunit having a molecular weight of about 75,000.+-.3,000 Da, wherein the aldehyde dehydrogenase is obtained from a microorganism belonging to the genus Gluconobacter;has L-sorbosone, D-glucosone, D-glucose, D-galactose, D-mannose, L-gulose, D-xylose, D-ribose, and D-arabinose dehydrogenase activity;utilizes as cofactors pyrroloquinoline quinone and heme c;has an optimum pH of from 7.0 to 8.5; andis inhibited by Co.sup.2+, Cu.sup.2+, Fe.sup.2+, Ni.sup.2+, Zn.sup.2+, monoiodoacetate and ethylenediamine tetraacetic acid.