Patent ID: 6333149
Filing Date: 2001-12-25
Classification: C12Q,G01N,Y10T

Abstract:
A method for identifying an atom of a common ligand mimic that is proximal to an interface region;wherein the enzyme binds a common ligand (CL) or a common ligand mimic (CL mimic) at a common ligand site (CL site) and binds a specificity ligand (SL) at an adjacent specificity ligand site (SL site);wherein an interface region is defined as the atoms of the enzyme between the CL site and SL site, and atoms of an SL if bound to the enzyme;wherein the enzyme catalyzes a reaction mechanism involving the SL and a reactive atom of the CL; andwherein a CL reactive region is defined as the reactive atom of the CL and immediately adjacent atoms of the CL;comprising the steps of(a) identifying an atom of the interface region, comprising the steps of(1) binding a CL to the CL site of the enzyme;(2) perturbing an atom of the CL reactive region; and(3) identifying an NMR cross-peak corresponding to an atom that is perturbed by the perturbation of the atom of the CL reactive region, thereby identifying an atom of the interface region; then(b) identifying an atom in the CL mimic that is proximal to the interface region, comprising the steps of(1) binding a CL mimic to the CL site;(2) perturbing th e interface atom identified in step (a); and(3) identifying an NMR cross-peak corresponding to an atom of the CL mimic that is perturbed by the perturbation of the interface atom, thereby identifying an atom of the CL mimic that is proximal to the interface region.