Patent ID: 6310199
Filing Date: 2001-10-30
Classification: B01J,C12N

Abstract:
A pH dependent ion exchange matrix for isolating a target nucleic acid, comprising:a silica magnetic particle; anda plurality of first ion exchange ligands, each first ion exchange ligand comprising:an aromatic hydrocarbon ring, wherein at least one member of the ring is an amine with a pK of less than about 9;a spacer covalently attached to the aromatic hydrocarbon ring, the spacer comprising a spacer alkyl chain of with an amine terminus, and an acidic moiety covalently attached to the spacer alkyl chain; anda linker comprising a linker alkyl chain covalently attached to the silica magnetic particle through a silica residue at a first end of the linker alkyl chain and covalently attached to the amine terminus of the spacer at a second end of the linker alkyl chain;wherein the matrix has a capacity to adsorb to a target nucleic acid at a first pH, and to release the target nucleic acid at a desorption pH which is higher than the first pH.