Patent ID: 8999680
Filing Date: 2015-04-07
Classification: C12P

Abstract:
1. An in vitro method for the preparation of 1,4-diaminobutane [DAB] comprising a) enzymatically preparing N-acetyl DAB which comprises a step of contacting N (b) recovering the N-acetyl-DAB from the enzymatic reaction mixture, and (c) converting the recovered-N-acetyl-DAB into DAB in an in vitro process comprising at least one further step which is an enzymatic conversion comprising contacting N-acetyl DAB with a hydrolytic enzyme selected from the group consisting of carboxylic ester hydrolases (E.C. 3.1.1), thiolester hydrolases (E.C. 3.1.2) and peptidases (E.C 3.4), or a chemical conversion involving deacylation by (PhO)