Patent ID: 6255047
Filing Date: 2001-07-03
Classification: C12Q,G01N

Abstract:
A method for preparing a fluid containing carbohydrate-deficient transferring at a preselected concentration for use as a reference in determinations of carbohydrate-deficient transferring (CDT) levels in human bodily fluids in which elevated levels of CDT are an indication of chronic overconsumption of alcoholic beverages, said method comprising:(a) contacting a fluid selected from the group consisting of a transferring-containing human bodily fluid and a transferring-containing fraction of a human bodily fluid with a neuraminidase thereby causing digestion of a majority of transferring in said fluid to CDT by said neuraminidase,(b) recovering said fluid from said neuraminidase,(c) quantifying the amounts of CDT and transferring remaining in said fluid, and(d) combining said recovered fluid with a matrix human bodily fluid having known CDT and transferring levels in order to create said reference with known CDT and transferring levels.