Patent ID: 6121008
Filing Date: 2000-09-19
Classification: G01N,Y10S

Abstract:
A chromatographic device for determining the presence or amount of analyte in a sample comprising:a) a sample receiving site,b) a mobilization zone in liquid communication with and spatially distinct from the sample receiving site,wherein the mobilization zone comprises liquid-mobilizable labelled multivalent receptors,wherein the liquid-mobilizable labelled multivalent receptors have at least one binding site which binds to the analyte in the sample to form receptor:analyte complexes, andc) at least two separate trap zones downstream of and in liquid communication with the mobilization zone,wherein each trap zone comprises at least one different immobilized ligand which binds to an analyte binding site of the liquid-mobilizable labelled multivalent receptors with an avidity or affinity different than the avidity or affinity of the immobilized ligand to an analyte binding site of the liquid-mobilizable labelled multivalent receptors in the other trap zone,wherein the avidity or affinity of the immobilized ligand increases in each trap zone further downstream of the mobilization zone, andwherein the presence or amount of the analyte in the sample is determined from the distance over which the receptor:analyte complexes migrate through the trap zones.