Patent ID: 6177553
Filing Date: 2001-01-23
Classification: C07H

Abstract:
A method for forming a sulfur-linked di- or oligosaccharide on a solid support which method comprises:i) immobilizing a first saccharide or oligosaccharide on a solid support wherein the first saccharide or oligosaccharide comprises a protected thiol group of the formula --SR at the anomeric carbon atom of the reducing saccharide unit wherein R is a thiol group protecting agent;ii) deprotecting the thiol group and optionally converting the deprotected thiol group to the corresponding thiolate;iii) optionally adding a metal complexing agent; andiv) contacting the immobilized saccharide or oligosaccharide group formed in ii) or iii) above with a second saccharide or oligosaccharide comprising a nucleophilic displaceable group in an inert solvent wherein said immobilized saccharide or oligosaccharide forms a solid phase in said solvent and further wherein said contacting is conducted under conditions wherein the thiol or thiolate group displaces said nucleophilic displaceable group thereby forming a sulfide linkage between the first and second saccharides/oligosaccharides.