Patent ID: 7638325
Filing Date: 2009-12-29
Classification: A61K,C12N

Abstract:
1. A K562 cell engineered to induce long term expansion of T cells, wherein said engineered K562 cell comprises on its surface: an anti-CD3 antibody loaded onto a human Fcγ receptor, wherein said human Fcγ receptor is expressed from an expression vector in said engineered K562 cell; a ligand for CD28, wherein said ligand for CD28 is selected from the group consisting of anti-CD28 antibody, CD80, and CD86, and wherein said ligand for CD28 is capable of cross-linking CD28 on T cells and delivering a secondary signal to said T cells; and wherein said K562 cell is further genetically modified to express the co-stimulatory molecule, 4-1BBL.