Patent ID: 6465410
Filing Date: 2002-10-15
Classification: C11D

Abstract:
A modified protein which comprises a catalytically active amino acid sequence of an antimicrobial enzyme and/or an amino acid sequence of an antimicrobial peptide, linked to an amino acid sequence comprising a cellulose binding domain wherein said catalytically active amino acid sequence derives from an antimicrobial enzyme selected from the group consisting of: Exo-&agr;-N acetyl gluosaminidase, Endo-&bgr;-N-acetyl glucosaminidase, N-acetyl-&bgr;-glucosaminidase, Dextranase, Lyticase; Xanthine oxidase, Protocatechuate 3,4 oxidoreductase, Catechol dioxygenase, Lipoxygenases, Hexosaminidase and combinations thereof;further wherein said cellulose binding domain is selected from the group consisting of CBD CenC, CenA, Cex from Cellulomonas fimi, GBD CBHI from Trichoderma reesei, CBD Cellulozome from Clostridium cellulovarans, CBD E3 from Thermonospora fusca, CBD-dimer from Clostridium stecorarium XynA, CBD from Bacillus agaradherens, CBD family 45 from Humicola insolens and mixtures thereof; further wherein said catalytically active amino acid sequence of an antimicrobial enzyme and/or amino acid sequence of an antimicrobial peptide is linked to said amino acid sequence comprising a cellulose binding domain, via an amino or non-amino linking region selected from the group consisting of: polyethylene glycol derivatives, nucleophilic polyethylene glycol derivatives, carboxyl polyethylene glycol derivatives, electrophilically activated polyethylene glycol derivatives, sulfhydryl-selective polyethylene glycol derivatives, heterofunctional polyethylene glycol derivatives, biotin polyethylene glycol derivatives, vinyl polyethylene glycol derivatives, silane polyethylene glycol derivatives, phospholipid polyethylene glycol derivatives, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, N-ethyl-5-phenylisaoxolium-3-sulphonate, 1-cylohexyl-3(2-morpholineothyl) carbodide metho-p-toluene sulphonate, N-ethoxycarbonyl-2-ethoxy 1,2 dihydroquinoline or glutaraldehyde, Humicola insolens family 45 cellulase linker, NifA gene of Kiebsiella pneumoniae-CiP linker, E. coli OmpA gene-CiP liner, E3 cellulase Thermomonospora fusca linker, CenA cellulase linker and combinations thereof.