Patent ID: 7544500
Filing Date: 2009-06-09
Classification: A61K,C12N,C12Y

Abstract:
1. A method for preparing a plasmin solution comprising: cleaving a plasminogen in the presence of a plasminogen activator to yield an active plasmin; substantially removing the plasminogen activator from the active plasmin by binding the active plasmin to an active plasmin-specific absorbent material to form a bound plasmin wherein the active plasmin-specific absorbent material comprises benzamidine, and eluting the bound plasmin with an excipient solution having a pH from about 2.5 to about 9.0 to form a plasmin solution; and buffering the plasmin solution with a low pH, low buffering capacity agent to form a reversibly inactive acidified plasmin solution having a pH of approximately 1 to 4.