Patent ID: 8076157
Filing Date: 2011-12-13
Classification: G01N,H01J,Y10T

Abstract:
1. A method for determining the amount of one or more vitamin D metabolites in a sample by mass spectrometry, the method comprising the steps of (i) subjecting the sample to 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD) under conditions sufficient to generate one or more PTAD-vitamin D metabolite derivatives; (ii) ionizing one or more PTAD-vitamin D metabolite derivatives in the sample to generate one or more ions detectable by mass spectrometry, wherein said ionizing comprises volatilizing said one or more PTAD-vitamin D metabolite derivatives by laser diode thermal desorption; (iii) determining the amount of one or more of the PTAD-vitamin D metabolite derivative ions by mass spectrometry; and (v) relating the amount of PTAD-vitamin D metabolite derivative ions determined in step (iv) to the amount of a vitamin D metabolite in the sample; wherein said one or more vitamin D metabolite derivatives are selected from the group consisting of 25-hydroxyvitamin D