Patent ID: 8465988
Filing Date: 2013-06-18
Classification: G01N,Y10T

Abstract:
1. A method for determining antioxidant activity of a sample comprising: adding a sample to intact red blood cells under conditions which permit the sample to cross the cell membrane of the intact red blood cells; washing the red blood cells to remove sample which has not crossed the cell membrane of the red blood cells; lysing the washed red blood cells; adding a fluorescent indicator dye to the lysed red blood cells, wherein the fluorescence indicator dye exhibits oxidation-sensitive changes in fluorescence intensity; inducing oxidative stress in the lysed red blood cell mixture; and detecting fluorescence intensity in the lysed red blood cell mixture using a microplate reader, wherein a decrease in the fluorescence intensity of the fluorescence indicator dye in the lysed red blood cells in the presence of the sample as compared to a control comprising lysed red blood cells which have not been treated with the sample or lysed red blood cells treated with a standard with known antioxidant activity indicates the antioxidant activity of the sample.