Patent ID: 7141262
Filing Date: 2006-11-28
Classification: A23J,A23V,A61K,A61P,C07K,Y10S

Abstract:
1. A method for obtaining a milk aqueous phase protein fraction enriched in TGFβ in activated form from a solution rich in milk aqueous phase soluble proteins, said method comprising the steps of: a) adjusting the protein concentration of said solution rich in milk aqueous phase soluble proteins to a concentration between 5 and 30 g/liter of solution; b) precipitating a part of said milk aqueous phase by acid treatment of said solution at a pH ranging between 4 and 5.5, at a temperature ranging between 55° C. and 68° C., and for a period of time between 30 seconds to 10 minutes, so as to obtain a precipitated suspension; c) carrying out microfiltration on the precipitated suspension, so as to obtain a microfiltration retentate; d) carrying out diafiltration on the microfiltration retentate, so as to obtain a diafiltrated microfiltration retentate; e) recovering said diafiltrated microfiltration retentate comprising said milk aqueous phase protein fraction enriched in TGFβ; and f) drying said diafiltrated microfiltration retentate, to obtain a powder enriched in TGFβ.