Patent ID: 7981656
Filing Date: 2011-07-19
Classification: A61K,C12N,Y10S

Abstract:
1. A pseudotyped retrovirus comprising a recombinant RNA associated with a retroviral core surrounded by a lipid bilayer having disposed therein modified Ebola glycoprotein, wherein the modified glycoprotein contains a deletion of amino acids 309-489 in the O-glycosylation region of a wild-type Ebola glycoprotein encoded by SEQ ID NO:1, wherein the recombinant RNA comprises (i) a nucleotide sequence defining a selected biomolecule intended for delivery to a target cell, and (ii) retroviral control elements for packaging, reverse transcription and integration of the retrovirus into a target cell, and wherein the pseudotyped retrovirus has a transduction efficiency into a target cell of at least 2-fold higher than a retrovirus pseudotyped with the wild-type Ebola glycoprotein.