Patent ID: 8709819
Filing Date: 2014-04-29
Classification: C07B,C07C,G01N,Y10T

Abstract:
1. A method for detecting the content of L-carnitine or D-carnitine in a sample comprising L-carnitine or D-carnitine, wherein the method includes following steps: (a) preparing a solution comprising the sample containing a proper amount of L-carnitine (or D-carnitine) and a control solution containing DL-carnitine; (b) mixing a proper amount of a derivative reagent and the test sample solution containing L-carnitine (or D-carnitine), and allowing them to react to generate L-carnitine (or D-carnitine) derivatives; wherein the derivative reagent has a structure of: wherein: the carbon atom marked with an asterisk is the chiral carbon atom; the compound is chiral compound having pure optical active, the D- or L-compound; R represents C1-C6 straight-chain or branched alkyl groups, C6-C10 aryl groups, C2-C6 straight-chain or branched alkenyl or alkynyl groups or C3-C6 cycloalkyl groups; and X represents a halogen atom; (c) subjecting the reacted test sample solution to HPLC to detect and thereafter calculating the content of L-carnitine (or D-carnitine) in the sample.