Patent ID: 8486865
Filing Date: 2013-07-16
Classification: C12N,C12Q,C40B

Abstract:
1. A method for detecting a plurality of mRNA molecules bound to at least one ribosome, said method comprising: (i) contacting said plurality of mRNA molecules with an enzymatic degradant or a chemical degradant thereby forming a plurality of RNA fragments, wherein each RNA fragment comprises an RNA portion protected from said enzymatic degradant or said chemical degradant by a ribosome to which the RNA portion is bound; (ii) amplifying said RNA fragments comprising said RNA portions to form a detectable number of amplified nucleic acid fragments; and (iii) detecting said detectable number of amplified nucleic acid fragments, thereby detecting an amount of two or more of said RNA portions and correlating said amount of two or more of said RNA portions to a rate of translation of one or more of said plurality of mRNA molecules.