Patent ID: 6391633
Filing Date: 2002-05-21
Classification: A61K,C07K,C12N,C12P

Abstract:
An isolated human cell, which contains a copy of an endogenous EPO gene in operable linkage with a heterologous promoter active in the human cell and produces at least 200 ng EPO/106 cells/24 h and wherein said endogenous EPO gene is not amplified and wherein the EPO gene has a signal peptide-coding sequence which codes for a signal peptide sequence wherein the sequence of the first 4 amino acids of said signal-peptide sequence is:Met-X1-X2-X3 wherein X1 is Gly or Ser, X2 is Ala, Val, Leu, Ile, Ser or Pro, and X3 is Pro, Arg, Cys or His, provided that X1-X2-X3 is not the sequence Gly-Val-His.