Patent ID: 6110687
Filing Date: 2000-08-29
Classification: C07K,C12Q,G01N

Abstract:
A method of detecting an antigen comprising:immobilizing an antigen to a solid support;contacting the solid support with a first solution comprising a first antibody which binds said immobilized antigen:contacting said solid support with a second solution comprising an immunodendrimer, said immunodendrimer comprising an anti-first antibody having an oligonucleotide complexed thereto and a labeled dendritic polynucleotide hybridized to said oligonucleotide, wherein the anti-first antibody binds the first antibody, and wherein said labeled dendritic polynucleotide comprises a plurality of matrix polynucleotide monomers bonded together by hybridization and cross-linking, wherein each matrix polynucleotide monomer, prior to hybridization bonding has a linear, double stranded waist region having a first end and a second end, said first end terminating with two single stranded hybridization regions, each from a strand of the waist region, and said second end terminating with one or two single stranded hybridization regions, each from a strand of the waist region, and in said dendritic polynucleotide each matrix polynucleotide monomer is hybridization bonded and cross-linked to at least one other matrix polynucleotide monomer at at least one such hybridization region and when hybridization bonded and cross-linked to more than one such hybridization region of the same matrix polynucleotide monomer, there is an intermediate region where the two monomers are not hybridization bonded or cross-linked, and wherein the plurality of matrix polynucleotide monomers present does not exceed saturation of the labeled dendritic polynucleotide; anddetecting presence or amount of said antigen directly by detecting said labeled immunodendrimer.