Patent ID: 8637290
Filing Date: 2014-01-28
Classification: C12N,C12P,Y02E

Abstract:
1. An isolated, synthetic or recombinant polypeptide having a phospholipase activity, and: (a) comprising an amino acid sequence (b) comprising the polypeptide of (a) but lacking a signal sequence or a proprotein sequence; (c) further comprising a heterologous amino acid sequence; (d) comprising the polypeptide of (c), wherein the heterologous amino acid sequence comprises, or consists of a heterologous (leader) signal sequence, or a tag or an epitope; (e) comprising the polypeptide of any one of (a) to (d), wherein the polypeptide is glycosylated, or the polypeptide comprises at least one glycosylation site, (ii) the polypeptide of (i) wherein the glycosylation is an N-linked glycosylation or an O-linked glycosylation; (iii) the polypeptide of (i) or (ii) wherein the polypeptide is glycosylated after being expressed in a yeast cell; or (iv) the polypeptide of (iii) wherein the yeast cell is a (f) the polypeptide of any one of (a) to (e), wherein (i) the polypeptide further comprises additional amino acid residues between a signal sequence (leader sequence or leader peptide) and the enzyme, or (ii) the polypeptide of (i), wherein the additional amino acid residues comprise Glu-Ala.