Patent ID: 7282348
Filing Date: 2007-10-16
Classification: G01N,Y10S

Abstract:
1. A kit for measuring cytotoxicity of a test agent, the kit comprising, in combination: a dye disposed in a first container, the dye having an oxidized state and a reduced state wherein the reduced state can be distinguished from the oxidized state and wherein the dye is present in the oxidized state, wherein the dye is resazurin or 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulphophenyl)-2H-tetrazolium, inner salt (MTS), a substrate mix disposed in a second container, the substrate mix comprising an electron transfer agent, a substrate for a cytoplasmic enzyme having a half-life greater than two hours, wherein the substrate is lactate, and a cofactor for a cytoplasmic enzyme; wherein the substrate is oxidized upon contact with a cytoplasmic enzyme, and oxidation of the substrate drives reduction of the dye; and instructions for use of the kit.