Patent ID: 7638612
Filing Date: 2009-12-29
Classification: C12N,C12P,C12Q

Abstract:
1. A reagent mixture comprising a ready to use reagent solution that demonstrates prolonged stability when stored at −20° C., wherein said solution comprises: (a) glycerol in a concentration between about 10% and about 40%, and (b) a viral reverse transcriptase in a concentration sufficient for use in a reverse transcription reaction without adding additional reverse transcriptase, wherein said viral reverse transcriptase is selected from the group consisting of AMV RT, RSV RT, MMLV RT, HIV RT, EIAV RT, RAV2 RT, THERMOSCRIPT RT, ASLV and Rnase H in a buffer suitable foruse in a reverse transcription reaction, wherein said buffer further comprises: a metal ionnecessary for reverse transcriptase activity; nucleoside triphosphates, and wherein said buffer does not contain Taq polymerase in a concentration suitable for a subsequent PCR reaction.