Patent ID: 6455675
Filing Date: 2002-09-24
Classification: C12N,C12Y

Abstract:
A method of preparing a DNA sequence encoding a polypeptide exhibiting GA 20-oxidase activity, said DNA sequence comprising (a) a nucleotide sequence which encodes a polypeptide exhibiting GA 20-oxidase activity having the amino acid sequence of SEQ ID No 2, 4 or 6, or (b) a nucleotide sequence which hybridizes with the nucleotide sequence of (a) or its complement under hybridizing conditions and encodes a polypeptide exhibiting GA 20-oxidase activity, said method comprising preparing a cDNA library from a suitable source organism, screening said library by means of an antibody against GA 20-oxidase or part of its amino acid sequence, or by testing for catalytic activity characteristic of the GA 20-oxidase, and preparing said DNA sequence encoding the polypeptide exhibiting GA 20-oxidase activity based on the screening results.