Patent ID: 8530438
Filing Date: 2013-09-10
Classification: A01K,A61P,C12N

Abstract:
1. A method of inducing ribonucleic acid interference (RNAi) of a target gene in a mammalian cell in vivo, the method comprising (a) contacting the cell with an isolated nucleic acid molecule, said isolated nucleic acid molecule comprising a regulatory sequence operably linked to a nucleic acid sequence that encodes an engineered ribonucleic acid (RNA) precursor which is processed to form a small interfering ribonucleic acid (siRNA) comprising an antisense strand complementary to a sequence of a messenger RNA (mRNA) of the target gene and a sense strand having a sequence complementary to the antisense strand, wherein the precursor comprises (b) inducing the cell to express the engineered RNA precursor encoded by the nucleic acid molecule, wherein the precursor is processed by the cell to generate an siRNA that mediates cleavage of the mRNA and wherein the sequences flanking the antisense and sense strands in the stem portions facilitate processing of the precursor in the cell to form the siRNA, thereby inducing RNAi of the target gene in the mammalian cell in vivo.