Patent ID: 7521186
Filing Date: 2009-04-21
Classification: C12N,C12Q

Abstract:
1. A method of performing PCR and separating one or more PCR products, the method comprising: (i) mixing one or more PCR reaction components with an unpolymerized sieving medium in a channel of a microfluidic device to provide an unpolymerized PCR sieving medium within the channel; (ii) thermocycling the PCR sieving medium to produce one or more PCR products; (iii) polymerizing the sieving medium after thermocycling is completed, wherein the polymerized sieving medium has a polymer concentration that is less than 0.4%; and (iv) separating the one or more PCR products by flowing the one or more PCR products through the polymerized sieving medium.