Patent ID: 6436707
Filing Date: 2002-08-20
Classification: C12N,C40B

Abstract:
A vector comprising:a) a 5â€² gene trap cassette, comprising in operable combination: 1) a splice acceptor; 2) a first exon sequence located 3â€² to said splice acceptor, said first exon encoding a marker enabling the identification of a cell expressing said exon; and 3) a polyadenylation sequence defining the 3â€² end of said first exon; and b) a 3â€² gene trap cassette located 3â€² to said polyadenylation sequence comprising in operable combination: 1) a first promoter; 2) a second exon sequence located 3â€² from and expressed by said promoter, said second exon not encoding an activity conferring antibiotic resistance; and 3) a splice donor sequence defining the 3â€² region of the exon; and wherein said vector does not encode a promoter mediating the expression of said first exon, and wherein said vector does not encode a sequence that mediates the polyadenylation of an mRNA transcript encoded by said second exon sequence and expressed by said first promoter.