Patent ID: 6642000
Filing Date: 2003-11-04
Classification: B01J,C12Q,C40B

Abstract:
A method for performing PCR amplification of a nucleic acid molecule and parallel hybridization wherein amplified products are also identified by hybridizing them on the microchip within gel pads surrounded by a hydrophobic liquid, and analyzing melting curve patterns said method comprising:(a) providing a microchip with a plurality of immobilized and non-immobilized PCR primers for each gel pad; (b) adding a hybridization solution and the nucleic acid molecule to be tested to the microchip; (c) replacing the hybridization solution with the amplification solution; (d) modulating at least one of said immobilized primers; (e) replacing the amplification solution with a hydrophobic liquid, so that the hydrophobic liquid surrounds the gel pads; (f) cycling the temperature to achieve PCR amplification within each gel pad; (g) repeating steps (a) to (f) wherein the immobilized primers are activated, inactivated, or detached from the gel pads; and (h) identifying the amplification products by hybridization and melting curve analysis on the microchip.