Patent ID: 6861225
Filing Date: 2005-03-01
Classification: C12Q

Abstract:
1. A method for diagnosing the existence of a malignant process and/or for monitoring results of a therapy applied in its treatment by determining 5′-ribonucleotide phosphohydrolase activity, wherein a biological sample is incubated with a nucleotide pentose monophosphate as substrate, the liberated inorganic phosphate is converted into a colored complex by treating it with ammonium molybdate and a reducing agent, color intensity is measured, and from the measured value the activity of 5′-ribonucleotide phosphohydrolase or the amount of inorganic phosphate liberated within unit time, as a figure proportional to the activity of 5′-ribonucleotide phosphohydrolase is calculated, wherein 5′-AMP, 5′-CMP, 5′-UMP, 5-GMP, 5′-IMP and 5′-TMP are used as nucleotide pentose monophosphates, the measurement is performed on all of these six substrates, and the obtained results are compared with one another and with control values.