Patent ID: 9012219
Filing Date: 2015-04-21
Classification: A61K,C07K,C12N

Abstract:
1. A method for generating a reprogrammed cell colony comprising reprogrammed cells that exhibit a second differentiated state, comprising: introducing into human or mammalian cells that exhibit a first differentiated state a purified RNA preparation comprising in vitro-synthesized modified mRNA molecules that: (i) contain at least one pseudouridine that is not further modified and/or 1-methylpseudouridine in place of at least one unmodified uridine nucleoside, (ii) exhibit a 5′ cap, (iii) exhibit a 3′ poly(A) tail comprising 50-200 or greater than 150 nucleotides, and (iv) encode at least one reprogramming factor, wherein said purified RNA preparation is free of an amount of RNA contaminant molecules, including double-stranded RNA molecules, that would activate an immune response in said cells sufficient to prevent survival of said cells for at least 20 days in culture and the generation of a reprogrammed cell colony; and repeating said introducing on multiple days, under conditions such that at least one reprogrammed cell colony is generated that comprises reprogrammed cells that exhibit a second differentiated state.