Patent ID: 7544493
Filing Date: 2009-06-09
Classification: A61P,C07K

Abstract:
1. A method for the production of a polypeptide having the amino acid sequence of SEQ ID NO: 2, comprising: (a) expressing a nucleic acid encoding said polypeptide in a microbial host cell, (b) isolating inclusion bodies containing said polypeptide in denatured form, (c) solubilizing the inclusion bodies at a pH of 7-9 in a phosphate buffered solution comprising a denaturing agent, and (d) renaturing the denatured polypeptide at a pH of 7-9 in a phosphate buffered solution comprising reduced glutathione (GSH) and oxidized glutathione (GSSG) and a denaturing agent in a non-denaturing concentration.