Patent ID: 6277632
Filing Date: 2001-08-21
Classification: C07H,C12N

Abstract:
A kit for the production of nucleic acid multicomponent constructs, comprising a package containing at least three nucleic acid components, including a first nucleic acid component selected from a category of first nucleic acid components having a common biological utility or functionality, a second nucleic acid component selected from a category of second nucleic acid components having a common biological utility or functionality, and a third nucleic acid component selected from a category of third nucleic acid components having a common biological utility or functionality and, optionally, at least one oligonucleotide bridge,wherein the biological utility or functionality of each of the first, second and third categories is different from the other categories,wherein at least two of the at least three nucleic acid components are supplied as a library of two or more different nucleic acid fragments, andwherein each of the first, second, and third nucleic acid components comprises a double stranded nucleic acid molecule having at least one unique non-palindromic single stranded 5' or 3' terminal sequence, which allows for specific annealing and linkage of the first, second, and third nucleic acid component, and optionally the at least one oligonucleotide bridge, in a predetermined order.