Patent ID: 7176005
Filing Date: 2007-02-13
Classification: C07K,C12N,C12P

Abstract:
1. A method of hydrogen gas generation, comprising the steps of: culturing genetically modified algae under illuminated conditions wherein sulfate permease expression of the algae, comprising SEQ ID NO:1, is reduced relative to unmodified wild-type algae wherein the algae is genetically modified to disrupt expression of chloroplast sulfate permease gene CrcpSulP comprising SEQ ID NO:2 by insertion of an antisense sequence which hybridizes under high stringency conditions, wherein said conditions are hybridization and/or washing conditions at 68° C. in 0.2. times. SSC or at 42° C. in 50% foramide, 4. times SSC to a portion of SEQ ID NO:2 mRNA; sealing the algae culture from atmospheric oxygen; and collecting hydrogen gas evolved.