Patent ID: 6468740
Filing Date: 2002-10-22
Classification: B01J,B82Y,C07B,C07H,C07K,C40B,G03F,Y02P

Abstract:
A method of synthesizing an array of oligonucleotides for analyzing a target nucleic acid, comprisingdetermining oligonucleotide sequences complementary to the same nucleic acid monomer sequence of a target nucleic acid except at a single position in the target nucleic acid, the oligonucleotide sequences thereby forming a plurality of groups, where the oligonucleotide sequences within the same group are substituted with each member of a basis set of four different nucleotides at the same single position, and the single position differing between groups, and the target nucleic acid being of known sequence; and thereafter assembling the oligonucleotides having the determined sequences on a support, the different oligonucleotides of each group occupying distinct known locations on the support.