Patent ID: 8263404
Filing Date: 2012-09-11
Classification: C12N,G01N

Abstract:
1. A method of separating a desired cell type from an erythrocyte-containing cell suspension containing both erythrocytes and the desired cell type, comprising: adding an agglutinating agent to the erythrocyte-containing cell suspension at a concentration that causes erythrocytes to agglutinate, wherein said agglutinating agent does not bind to the desired cell type; wherein said agglutinating agent is an antibody, wherein said antibody has the following properties: (a) the antibody binds to an erythrocyte cell surface molecule, (b) the antibody does not bind to the desired non-erythrocyte cell type and (c) said antibody causes erythrocytes to agglutinate and form erythrocyte-antibody agglutinates including said antibody; regularly mixing the cell suspension; and separating the erythrocyte-antibody agglutinates from the desired cell type in the cell suspension in a liquid of a homogeneous density.