Patent ID: 6476000
Filing Date: 2002-11-05
Classification: A61K,A61P,C12N

Abstract:
A method for reducing the immunostimulatory effect of a CpG-containing oligonucleotide, the method comprising introducing a 2â€²-substituted nucleoside into the oligonucleotide at a position adjacent to, and on the 5â€²side of the CpG dinucleoside, wherein at least one nucleoside is a deoxyribonucleoside, and the oligonucleotide is not complementary to the gag or rev gene of human immunodeficiency type 1, wherein only one 2â€² substituted nucleoside is introduced into the oligonucleotide for each CpG dinucleotide present in the oligonucleotide, thereby producing an oligonucleotide having a reduced immunostimulatory effect relative to a CpG-containing oligonucleotide that does not comprise a 2â€²-substituted nucleoside at a position adjacent to, and on the 5â€² side of the CpG dinucleoside.