Patent ID: 7713704
Filing Date: 2010-05-11
Classification: G01N

Abstract:
1. A method of identifying an organic or an inorganic molecule that binds specifically to MN's cell adhesion site, to which site vertebrate cells adhere in a cell adhesion assay, wherein said site is within MN's proteoglycan-like domain, and wherein said site's amino acid sequence comprises an amino acid sequence selected from the group consisting of SEQ ID NOS: 10 and 98-103, said method comprising testing an organic or an inorganic molecule in a cell adhesion assay, wherein said cell adhesion assay comprises: (a) allowing MN protein, which comprises said site, and/or MN polypeptide, which comprises said site, to bind to a substrate, to which substrate vertebrate cells do not bind; (b) rinsing unbound MN protein or unbound MN polypeptide from said substrate; (c) incubating the bound MN protein or the bound MN polypeptide with said organic or inorganic molecule, and with said vertebrate cells; (d) rinsing unbound vertebrate cells from said bound MN protein or bound MN polypeptide; and (e) if said organic or said inorganic molecule inhibits the adhesion of said vertebrate cells to said MN protein or to said MN polypeptide, identifying said molecule as specifically binding to said site; wherein said site, and said MN protein or said MN polypeptide, are specifically bound by the M75 monoclonal antibody that is secreted from the hybridoma VU-M75, which was deposited at the American Type Culture Collection under ATCC No. HB 11128, and wherein said MN protein or said MN polypeptide is encoded by a nucleotide sequence selected from the group consisting of: (i) SEQ ID NO: 1; (ii) nucleotide sequences that hybridize specifically under stringent hybridization conditions of 0.02 M to 0.15 M NaCl at temperatures of 50° C. to 70° C. to the complement of SEQ ID NO: 1; and (iii) nucleotide sequences that differ from SEQ ID NO: 1 or from the nucleotide sequences of (ii) in codon sequence due to the degeneracy of the genetic code; and wherein if said MN protein or said MN polypeptide is a fusion protein or a fusion polypeptide, the non-MN portion of said fusion protein or said fusion polypeptide does not contain a cell adhesion site.