Patent ID: 6017733
Filing Date: 2000-01-25
Classification: C07K,C12N

Abstract:
A method for expressing an immunoglobulin protein or antigen binding fragment thereof comprising(1) transforming a mammalian cell with an expression vector comprising(a) a dominant selectable marker wherein the translation initiation start site of said marker comprises the following sequence: -3 +1 Pyxx ATG Pyxx wherein ""Py"" is a pyrimidine nucleotide, ""x"" is a nucleotide, and the numerical designations are relative to the codon ""ATG"", and(b) a DNA sequence or sequences encoding said immunoglobulin protein or fragment thereof, wherein said DNA sequence or sequences are operably linked to regulatory sequences that provide for the expression of said immunoglobulin protein or fragment thereof in a mammalian cell transformed with said expression vector;(2) and culturing the resultant transformed mammalian cell under conditions that provide for the expression of said immunoglobulin or fragment thereof.