Patent ID: 8580510
Filing Date: 2013-11-12
Classification: C12N,C12Q

Abstract:
1. A nucleic acid amplification system for the selective amplification of at least one target nucleic acid sequence from a nucleic acid sample, said amplification system comprising: (a) a DNA polymerase; (b) a tagged oligonucleotide comprising: (c) a tag closing sequence which hybridizes to said target hybridizing sequence under a second set of conditions, thereby blocking hybridization of said tagged oligonucleotide to said target nucleic acid sequence, wherein said tag closing sequence does not stably hybridize to said target hybridizing sequence under said first set of conditions; and (d) a first priming oligonucleotide which hybridizes to the complement of said tag sequence under said second set of conditions so that said first priming oligonucleotide can be extended in a template-dependent manner in the presence of the DNA polymerase.