Patent ID: 7547541
Filing Date: 2009-06-16
Classification: A61P,C12M,C12N

Abstract:
1. A system for in vitro producing a mammalian pre-embryo, said system comprising means for obtaining a mammalian oocyte, and means for obtaining a mammalian spermatozoa, and an apparatus having at least two separate air-tight chambers, for which the oxygen tension of one chamber may be changed independent of the oxygen tension of the other chamber, said at least two separate air-tight chambers constitute a main chamber and at least one residence chamber, where said at least one residence chamber are smaller than said main chamber, and are located inside the main chamber and/or are attached to the main chamber, said apparatus comprising at least one entrance port capable of communicating with the means for obtaining the mammalian oocyte and/or the mammalian spermatozoa, and an airlock chamber, communicating with but separate from said main chamber, and separate from said residence chamber(s), for withdrawal of the pre-embryo and for transport of culturing means to and from said main chamber, as well as at least one communication port, each residence chamber communicating, through at least one such communication port, with said main chamber, allowing transfer of oocyte, spermatozoa and/or pre-embryo between said main chamber and each said at least one residence chamber.