Patent ID: 9096893
Filing Date: 2015-08-04
Classification: C12Q

Abstract:
1. A method of analyzing a target nucleic acid, comprising: a) amplifying a target nucleic acid in the presence of a synthetic probe and a thermostable FEN-1 endonuclease under conditions such that said synthetic probe is cleaved during an amplification reaction to generate cleaved fragments; wherein said synthetic probe is a footprint probe comprising an analyte-specific portion and a non-target portion, wherein said non-target portion is substantially non-complementary to said target nucleic acid and wherein said analyte-specific portion is no more than 12 nucleotides in length, and contains up to 12 nucleotides that are complementary to said target nucleic acid, and wherein, if said amplifying is done in an isothermal reaction, said analyte-specific portion of said footprint probe has a calculated T b) detecting said cleaved fragments during said amplification reaction.