Patent ID: 7541165
Filing Date: 2009-06-02
Classification: C12Q

Abstract:
1. A method for detecting a single stranded DNA or RNA target polynucleotide in a test sample, said method comprising: (a) hybridizing said single stranded target polynucleotide with a target-specific linker of an abortive promoter cassette, wherein said abortive promoter cassette comprises (b) incubating said hybridized target polynucleotide and linker of part (a) with an RNA polymerase, an initiator, and a terminator; (c) synthesizing an oligonucleotide transcript that is complementary to the initiation start site of said abortive promoter cassette by an abortive, reiterative process, wherein said process does not use said single-stranded target polynucleotide as a template and wherein said initiator is extended until said terminator is incorporated into said oligonucleotide transcript, thereby synthesizing multiple abortive reiterative oligonucleotide transcripts; and (d) detecting or quantifying said reiterative oligonucleotide transcripts.