Patent ID: 8932822
Filing Date: 2015-01-13
Classification: G01N

Abstract:
1. A method for identifying an agent B that binds to an allosteric site on a target protein, the method comprising: a. contacting the target protein with an agent A that binds to an active site of the target protein or wherein agent A is naturally bound to the active site of the target protein when isolated; b. contacting the target protein with the agent B, wherein the target protein is labeled with a second harmonic-active moiety having a net orientation at an interface, wherein a detectable signal is generated by the second harmonic-active moiety using a surface selective technique, and wherein the detectable signal indicates a conformational change in the structure of the target protein produced when the agent B binds to an allosteric site on the target protein; and c. measuring the presence or absence of the detectable signal after the target protein has been contacted with the agent B.