Patent ID: 8232047
Filing Date: 2012-07-31
Classification: C12Q,G01N

Abstract:
1. A method for conducting a luciferase-based assay comprising, expressing within a eukaryotic cell a luciferase that is secreted in its native form, wherein the luciferase lacks a functional signal peptide such that it is expressed intracellularly in the eukaryotic cell; recovering the activity of the luciferase by contacting the luciferase with a reagent composition that provides an environment suitable for conversion of the luciferase into its active folded form, wherein the reagent composition comprises an agent selected from the group consisting of bromide anion, chelator, redox buffer, and buffer with a pH greater than 8.0; contacting the luciferase with a substrate of the luciferase enzyme; and detecting or measuring the bioluminescence.