Patent ID: 7267947
Filing Date: 2007-09-11
Classification: C12Q,G01N,Y10S

Abstract:
1. A method of screening for the presence of breast cancer cells that have an increase in copy number of a target polynucleotide on chromosome 20 in a sample, the method comprising: contacting a nucleic acid sample from a human patient with a probe which binds selectively under hybridization conditions comprising 50% formamide, 2×SSC at 37° C. and wash conditions comprising 50% formamide, 2×SSC at 45° C. for 10 minutes to a target polynucleotide sequence on a region of chromosome 20 in which copy number is altered in breast cancer cells detecting the formation of a hybridization complex, thereby detecting breast cancer cells that have an increase in copy number of the target polynucleotide sequence.