Patent ID: 8124424
Filing Date: 2012-02-28
Classification: G01N

Abstract:
1. A single molecule-format bioluminescent probe for detecting a target-specific ligand in a living cell, which comprises, a ligand-binding molecule of which conformation is changed upon binding to the ligand, wherein the ligand-binding molecule comprises a ligand-binding domain (LBD) of a nuclear receptor and an LBD-interacting domain that is a coactivator peptide of said nuclear receptor, and an N-terminal polypeptide and a C-terminal polypeptide of a click beetle luciferase (N-CBLuc and C-CBLuc), which flank each end of the ligand-binding molecule, respectively, wherein the N-CBLuc and the C-CBLuc self-complement to generate a luminescent signal only upon binding of the ligand to the ligand-binding molecule, and wherein N-CBLuc consists of the sequence of amino acids 1-412 of SEQ ID NO: 27 and C-CBLuc consists of the sequence of amino acids 413-542 of SEQ ID NO: 27, OR N-CBLuc consists of the sequence of C-CBLuc consists of the sequence of