Patent ID: 6660270
Filing Date: 2003-12-09
Classification: A61K,A61P,C07K,G01N

Abstract:
An isolated multiple epitope fusion polypeptide comprising the general structural formula (I):(A)xâ€”(B)yâ€”(C)zâ€ƒâ€ƒ(I) wherein(I) is a linear amino acid sequence; B comprises an amino acid sequence that corresponds to an antigenic determinant of a GapC protein; A and C each comprise an amino acid sequence that is (i) different from B, (ii) different from the other, and (iii) an amino acid sequence that corresponds to an antigenic determinant of a GapC protein wherein said antigenic determinant is not adjacent to B in nature; A, B and C each comprise epitopes from one or more bacterial species selected from the group consisting of Streptococcus dysgalactiae, Streptococcus agalactiae, Streptococcus uberis, Streptococcus parauberis, and Streptococcus iniae; y is an integer of 1 or more; and x and z are each independently integers wherein x+z is 1 or more, and further wherein A, B, and C each comprise amino acid sequences selected from the group consisting of (a) the amino acid sequences corresponding to amino acid positions 62 to 81, inclusive, of a Streptococcal GapC protein; (b) the amino acid sequences corresponding to amino acid positions 102 to 112, inclusive, of a Streptococcal GapC protein; (c)the amino acid sequences corresponding to amino acid positions 165 to 172, inclusive, of a Streptococcal GapC protein; (d) the amino acid sequences corresponding to amino acid positions 248 to 271, inclusive, of a Streptococcal GapC protein; and (e) the amino acid sequences corresponding to amino acid positions 286 to 305, inclusive, of a Streptococcal GapC protein.