Patent ID: 8691515
Filing Date: 2014-04-08
Classification: C12Q,G01N

Abstract:
1. A method for detecting a blood disorder in a subject, comprising: contacting a blood sample obtained from a subject with an amine reactive reagent, thereby modifying said blood sample; blocking any of the amine reactive reagent in the modified blood sample with a blocking reagent selected therefor; subsequently digesting the modified blood sample with a trypsin composition, thereby producing a digested blood sample comprising a first plurality of glycated N-terminal peptides and a second plurality of non-glycated N-terminal peptides; analyzing said digested blood sample with MALDI MS to detect therein and separate therefrom the first plurality of glycated N-terminal peptides and the second plurality of non-glycated N-terminal peptides; comparing and correlating said first plurality of glycated N-terminal peptides and the second plurality of non-glycated N-terminal peptides with at least one selected reference data set; and determining therefrom said comparison and correlation the presence or absence of the blood disorder.