Patent ID: 6043039
Filing Date: 2000-03-28
Classification: C12Q

Abstract:
A fluorescent in situ hybridization method comprising the steps of:(a) obtaining a chromosome spread of a species;(b) preparing a hybridization composite containing a plurality of chromosomal paints each of said plurality of chromosomal paints being labeled with a different fluorophore-or-combination-of-fluorophores, such that an average specific activity of highly repetitive sequences in said hybridization composite substantially equals an average specific activity of unique sequences in said hybridization composite;(c) denaturing said hybridization composite and subjecting said hybridization composite to conditions for allowing at least a part of said highly repetitive sequences in said hybridization composite to reanneal while at least a part of said unique sequence in said hybridization composite remaining single stranded;(d) contacting under hybridization conditions said hybridization composite with said spread;(e) washing away excess of said hybridization composite; and(d) analyzing and presenting images of said now hybridized chromosome spread;wherein said repetitive sequences present in said plurality of chromosomal paints are at least partially removed from said composite, such that they are under-represented as compared to genomic DNA.