Patent ID: 8748354
Filing Date: 2014-06-10
Classification: C12Q

Abstract:
1. A method of sample analysis comprising: a) cross-linking the contents of a cell using a crosslinking agent to produce cross-linked ribonucleotide complexes; b) fragmenting said cross-linked ribonucleotide complexes to produce complexes comprising protein, RNA fragments and genomic DNA fragments; c) contacting said complexes with a plurality of non-overlapping oligonucleotides that comprise an affinity tag and that are complementary to a specific target RNA of said cell under high stringency conditions; d) isolating complexes that contain said oligonucleotides using the affinity tag of said oligonucleotides to produce isolated complexes; e) treating the isolated complexes with RNAse and a protease, thereby enzymatically releasing said genomic DNA fragments from said isolated complexes to produce released genomic DNA; f) sequencing said released genomic DNA to identify an occupancy site for an RNA.