Patent ID: 6258552
Filing Date: 2001-07-10
Classification: G01N

Abstract:
A method for estimation of change in bone mineral density, comprising the steps of:(1) bringing a blood sample into contact with a solid carrier on the surface of which an anti soluble interleukin-6 receptor (sIL-6R) antibody (first antibody) has been immobilized;(2) bringing into contact with the solid carrier from the step (1)(i) an anti sIL-6R antibody (second antibody) bound to a detectable marker, or(ii) an anti sIL-6R antibody (second antibody) capable of binding to a detectable marker, followed by bringing a detectable marker into contact with the anti sIL-6R antibody (second antibody); and(3) measuring the amount of the detectable marker immobilized on the solid carrier, wherein the amount of the immobilized detectable marker relates to an amount of sIL-6R in the blood sample, and the amount of sIL-6R relates to the bone mineral density.