Patent ID: 6027880
Filing Date: 2000-02-22
Classification: B01J,B82Y,C07B,C07H,C12Q,C40B

Abstract:
An array of nucleic acid probes immobilized on a solid support, the array comprising at least two sets of probes,(1) a first probe set comprising a plurality of probes, each probe comprising a segment of at least six nucleotides exactly complementary to a subsequence of a reference sequence, the segment including at least one interrogation position complementary to a corresponding nucleotide in the reference sequence,(2) a second probe set comprising a corresponding probe for each interrogation position in the first probe set, each probe being identical to a sequence comprising a corresponding probe from the first probe set or a subsequence of at least six nucleotides thereof that includes the interrogation position, except that the interrogation position is occupied by a different nucleotide in each of the two corresponding probes from the first and second probe sets;wherein the probes in the first probe set have at least three interrogation positions respectively corresponding to each of three contiguous nucleotides in the reference sequence;provided that the array does not consist of a complete set of probes of a given length, wherein a complete set is all permutations of nucleotides A, C, G and T/U;wherein the reference sequence is from a CFTR gene.