Patent ID: 8551734
Filing Date: 2013-10-08
Classification: C12N

Abstract:
1. A method for selecting a target gene encoding a protein with ligase activity, comprising: (a) providing a library of polynucleotide fragments in which one or more polynucleotide fragments comprise: a target gene encoding a protein with ligase activity; (b) encapsulating the library of fragments in a plurality of aqueous droplets in an emulsion, wherein each of the aqueous droplets contains: (c) in the droplets, allowing the target gene from the library to be transcribed and translated such that the protein expressed by the target gene permits the polynucleotide fragment comprising the target gene to become covalently linked to the polynucleotide adaptor; and (d) amplifying the target gene by extending an adaptor-specific primer, wherein the target gene encoding a protein with ligase activity is selected.