Patent ID: 9193995
Filing Date: 2015-11-24
Classification: C12Q

Abstract:
1. A method of categorizing an immune response of an individual according to levels of a set of at least two IFN subtype transcripts, comprising the steps of: obtaining a sample from the individual; purifying RNA from the sample obtained from the individual and performing reverse transcription on the RNA from the sample to obtain cDNA; contacting the cDNA with a composition comprising at least a first oligonucleotide pair and a second oligonucleotide pair, wherein each oligonucleotide pair is capable of specifically binding to a single subtype of IFN transcript, wherein the first oligonucleotide pair is selected from the list of oligonucleotide pairs consisting of: SEQ ID NO: 30 and SEQ ID NO: 45; SEQ ID NO: 31 and SEQ ID NO: 46; SEQ ID NO: 32 and SEQ ID NO: 47; SEQ ID NO: 33 and SEQ ID NO: 48; SEQ ID NO: 34 and SEQ ID NO: 49; SEQ ID NO: 35 and SEQ ID NO: 50; SEQ ID NO: 36 and SEQ ID NO: 51; SEQ ID NO: 37 and SEQ ID NO: 52; SEQ ID NO: 38 and SEQ ID NO: 53; SEQ ID NO: 39 and SEQ ID NO: 54; SEQ ID NO: 40 and SEQ ID NO: 55; SEQ ID NO: 41 and SEQ ID NO: 56; SEQ ID NO: 42 and SEQ ID NO: 57; and SEQ ID NO: 43 and SEQ ID NO: 58; performing polymerase chain reaction on the cDNA, thereby simultaneously obtaining IFN transcript levels of the set of IFN transcripts from the sample obtained from the individual; determining a pattern of IFN transcript levels; comparing the pattern of IFN transcript levels of the individual to a pattern of IFN transcript levels of a known immune response; and categorizing the immune response of the individual as corresponding to the known immune response if the pattern of IFN transcript levels of the individual substantially match the pattern of IFN transcript levels of the known immune response; or categorizing the immune response of the individual as not corresponding to the known immune response if the pattern of IFN transcript levels of the individual do not substantially match the pattern of IFN transcript levels of the known immune response.