Patent ID: 6270985
Filing Date: 2001-08-07
Classification: G01N,Y10S

Abstract:
An enzyme-linked immunosorbent assay (ELISA) diagnostic kit for the assay of A. pleuropneumoniae serotypes 5a and 5b antibodies in the serum of a pig comprising in separate packaging:a) a solid support having bound thereto a purified lipopolysaccharide A. pleuropneumoniae serotype 5 antigen for a specific binding to anti-A. pleuropneumoniae serotypes 5a or 5b antibodies present in the serum of the pig, said bound antigen being stabilized for at least 25 weeks at 4.degree. C. with phosphate buffered saline pH 7.2, containing BSA, 0.1% thimerosal and caprylic acid; andb) a detectably labeled conjugate which binds to the anti-A. pleuropneuizlolniae serotypes 5a or 5b antibodies bound to said antigen which is bound to said solid support.