Patent ID: 8003354
Filing Date: 2011-08-23
Classification: C12Q

Abstract:
1. A multiplex detection method, comprising (a) providing a first solid support comprising a plurality of different target nucleic acids, (b) providing a plurality of at least 100 different probe sets, wherein each of said probe sets comprises a first allele specific probe and a locus specific probe, (c) hybridizing said probe sets to said target nucleic acids and forming a plurality of hybridization complexes; (d) extending the first allele specific probe of each of said hybridization complexes in an allele specific manner under sufficient conditions so that only the first allele specific probe of each of said hybridization complexes that is perfectly complementary to the SNP allele of a target nucleic acid of said plurality of different target nucleic acids is extended, and forming extended probes; (e) ligating said extended probes to said locus specific probes and forming ligated probes; (f) amplifying said ligated probes and forming amplicons, each of said amplicons comprising said adapter sequence; (g) hybridizing said amplicons to capture probes on a second solid support having at least 100 different said capture probes, (h) detecting said amplicons that are hybridized to said capture probes; thereby detecting said single nucleotide polymorphism allele of each of said plurality of different target nucleic acids.