Patent ID: 6339172
Filing Date: 2002-01-15
Classification: G01N

Abstract:
A method for analyzing an objective substance, comprising reacting a labeled probe with an objective substance on a biological sample, said probe comprising a label substance of the formula (I): wherein A1 is an aromatic group, R1 is a hydrogen or â€”COCH2COCnF2n+1 and n is an integer of 1-6, or a label substance of the following formula (II): wherein A2 and A3 are the same or different and each is an aromatic group, R2 and R3 are the same or different and each is a hydrogen or â€”COCH2COCnF2n+1 and n is an integer of 1-6, bonded to a probe selected from the group consisting of nucleic acid, nucleic acid binding protein, low molecular ligand and receptor for ligand (except antibody) via a cross-linking group or a cross-linking group and a conjugating group, adding a heavy metal ion and assaying fluorescence of the resultant fluorescent complex, and wherein the biological sample is a member selected from the group consisting of a tissue, a cell and a chromosome.