Patent ID: 6686160
Filing Date: 2004-02-03
Classification: C12Q

Abstract:
A method of analysing genomic DNA comprising a repeat sequence in a sample, said method comprising:providing first and second oligonucleotide primers, said first oligonucleotide primer comprising a repeat sequence and at least one nucleotide inconsistent with the repeat pattern positioned on the first oligonucleotide primer's 5â€² end, where said nucleotide localizes the first oligonucleotide primer to the 5â€² end of a repeat sequence in the genomic DNA sample, and said second oligonucleotide primer starting within such a genetic distance on the 3â€² side of the repeat sequence in the genomic DNA sample that amplification of the genomic DNA can be performed; conducting a nucleic acid amplification on said genomic DNA in the sample using both the first and second oligonucleotide primers, thus producing DNA fragments based on repeat sequences on at least one end of the genomic DNA; and analyzing an amplified product thus produced to determine its length.