Patent ID: 8633000
Filing Date: 2014-01-21
Classification: C07C

Abstract:
1. A process for producing L-Citrulline, the process comprising: selecting a conversion material to be converted to L-Citrulline; dissolving the conversion material into an aqueous solution to obtain a conversion solution; inoculating the conversion solution with a microbial strain incubating the inoculated conversion solution for an interval sufficient to produce crude L-Citrulline from the conversion material; contacting resulting supernatant liquid that includes crude L-Citrulline with an adsorptive medium composed of activated carbon at a temperature above approximately 50° C. and below the temperature of denaturement for the L-Citrulline for an interval sufficient to remove at least one contaminant from the L-Citrulline; concentrating the L-Citrulline solution after removal of the adsorptive medium; crystallizing the L-Citrulline present in the concentrated liquid; dissolving resulting L-Citrulline crystals obtained in the step of crystallizing in at least an equal volume of a diluent; and contacting the resulting solution with a suitable adsorptive medium composed of activated carbon for an interval sufficient to remove at least one of discoloration and endotoxin.