Patent ID: 6670180
Filing Date: 2003-12-30
Classification: C12N,G01N

Abstract:
A method for expanding pancreatic islet cells in vitro without serum comprising introducing pancreatic islet cells into a cell culture medium or expanding cells in vitro, said culture medium comprising 1-150 mg/L arginine; 1-120 mg/L proline; 1-3050 mg/L nicotinamide; 0.1-100 mg/L transferrin chelated with iron; greater than 10âˆ’11M insulin or insulin-like growth factors; 10âˆ’12Mâˆ’10âˆ’3M glucocorticoid steroid; 1-6000 &mgr;g/L zinc salt; 1-250 &mgr;g/L manganese salt; 1-1000 &mgr;g/L copper salt; 1-150 &mgr;g/L selenium salt; 2.0-10.0 mM L-glutamine; 0.01-5.0 g/L D-galactose or 0.01-5.0 g/L D-glucose, or when both D-galactose and D-glucose are included together, 0.01-8.0 g/L and culturing said introduced cells in said medium.