Patent ID: 7105655
Filing Date: 2006-09-12
Classification: A61K,C07K,C12N

Abstract:
1. An isolated molecule containing a nucleotide sequence encoding an HIV-1 or HIV-2 envelope glycoprotein containing at least: i) a coiled coil portion of a gp41 transmembrane glycoprotein, wherein said coiled coil has a heptad repeat wherein each of said seven consecutive amino acid residues are designated a, b, c, d, e, f, and g corresponding to amino acid sequences selected from a group consisting of amino acids 555–561, 562–568, 569–575, 576–582 and 583–589 of SEQ ID NO: 11, wherein at least two amino acids in positions “a”, “d” and “e” have been substituted by cysteine residues, and “f” is glycine; and ii) a gp120 glycoprotein or gp120 derivative, wherein the gp120 derivative contains multiple gp120 constant regions connected by variable regions and/or linker residues that permit potential turns in the polypeptide structure so that the derivative maintains a conformation approximating that of wild type gp120, wherein at least a portion of one variable region has been deleted.