Patent ID: 6080552
Filing Date: 2000-06-27
Classification: G01N

Abstract:
A method of detecting active antibody production in response to target antigen in a blood sample, said method comprising:a) contacting at least one first aliquot of said sample in the absence of a protein synthesis inhibitor and at least one second aliquot in the presence of protein synthesis inhibitor, each aliquot with a solid phase under conditions which permit antibody production and secretion by a lymphocyte;b) generating a signal readable in solution and detecting the signal in solution to detect antibody binding to said antigen(s) on one or more solid phases in each aliquot; andc) comparing said signal of antibody binding from the first and second aliquots to determine the amount of active antibody secretion in response to said antigen(s), wherein the difference between the signals of the first and second aliquots indicate the antibody production with response to the target antigen(s).