Patent ID: 6713287
Filing Date: 2004-03-30
Classification: C07H,C12P

Abstract:
A method for synthesizing L-fucose in a single reaction mixture comprising the following steps:Step A: Providing L-fuculose-1-phosphate by an aldol addition reaction between dihydroxyacetone phosphate with DL-lactaldehyde, said aldol addition reaction being catalyzed by L-fuculose-1-phosphate aldolase; Step B: Converting the L-fuculose-1-phosphate of said Step A to L-fuculose, said conversion being catalyzed by acid phosphatase (E.C. 3.1.3.2); Step C: Converting the L-fuculose of said Step B to L-fuculose, said conversion being catalyzed by L-fucose isomerase, said L-fucose isomerase being purified recombinant L-fucose isomerase.