Patent ID: 7338780
Filing Date: 2008-03-04
Classification: C07K,C12N,C12P

Abstract:
1. A method for producing a peptide having three or more amino acid residues, comprising: forming the peptide having three or more amino acid residues with an enzyme or enzyme-containing substance, wherein the enzyme or enzyme-containing substance has an ability to use as substrates an amine component having two or more amino acid residues and a carboxy component, to form a peptide having one more peptide bond than the amine component; wherein said carboxy component is an amino acid ester or an amino acid amide; wherein said amine component is selected from the group consisting of an unprotected peptide having two or more amino acid residues, a C-protected peptide having two or more amino acid residues, and a peptide having two or more amino acid residues and having a C-terminal amine in place of an amino acid; wherein said carboxy component has an unprotected amino group; wherein said peptide having three or more amino acid residues contains an amino acid residue derived from said carboxy component at the N-terminus thereof; and wherein the enzyme or enzyme in said enzyme-containing substance is selected from the group consisting of a protein having the amino acid sequence consisting of amino acid residues numbers 21 to 619 of the amino acid sequence described in SEQ ID NO: 12, a protein having an amino acid sequence including substitution, deletion, insertion, and/or addition of one to ten of amino acids in the amino acid sequence consisting of amino acid residues 21 to 619 of the amino acid sequence described in SEQ ID NO: 12, a protein having the amino acid sequence described in SEQ ID NO: 12, a protein containing a mature protein region, the protein having an amino acid sequence including substitution, deletion, insertion, and/or addition of one to ten of amino acids in the amino acid sequence described in SEQ ID NO: 12, a product of a microbe that has been transformed so as to express a protein encoded by the polynucleotide consisting of nucleotides 121 to 1917 of the nucleotide sequence of SEQ ID NO: 11, and a product of a microbe that has been transformed so as to express a protein encoded by the polynucleotide consisting of nucleotides 61 to 1917 of the nucleotide sequence of SEQ ID NO: 11.