Patent ID: 6274320
Filing Date: 2001-08-14
Classification: C12Q

Abstract:
A method for sequencing a nucleic acid, the method comprising:providing one or more or more nucleic acid anchor primers linked to a solid support, wherein said solid support includes at least one optical fiber;providing a plurality of single-stranded circular nucleic acid templates;annealing an effective amount of the nucleic acid anchor primer to at least one of the single-stranded circular templates to yield a primed anchor primer-circular template complex;combining the primed anchor primer-circular template complex with a polymerase to generate an extended anchor primer covalently linked to a nucleic acid comprising multiple copies of a sequence complementary to the circular nucleic acid template;annealing an effective amount of a sequencing primer to one or more copies of said covalently linked complementary nucleic acid;extending the sequencing primer with a polymerase and a predetermined nucleotide triphosphate to yield a sequencing product and, if the predetermined nucleotide triphosphate is incorporated onto the 3' end of said sequencing primer, a sequencing reaction byproduct; andidentifying the sequencing reaction byproduct, thereby determining the sequence of the nucleic acid.