Patent ID: 6194209
Filing Date: 2001-02-27
Classification: G01N,Y10T

Abstract:
A system for detecting in vitro the pharmaceutically active ingredient of a pharmaceutical composition as absorbed from the gastrointestinal tract of a mammal comprising:a) a cross-section of gastrointestinal tissue harvested from a mammal selected from the group consisting of rats and rabbits, said section of tissue being so harvested such that said tissue retains a first side defined by the mucosa of such tissue and a second side defined by the muscularis of such tissue;b) a chamber for receiving and retaining said harvested tissue therein, said chamber being designed to retain said tissue such that said chamber and tissue cooperate to define first and second sub-chambers, said first chamber being defined by a portion of said container and said first side of said tissue, and said second chamber being defined by a portion of said chamber and said second side of said tissue;c) a first solution having a known concentration of said pharmaceutical composition dissolved therein, said solution being contacted with and stored within said first sub-chamber;d) a second solution comprising a biologically compatible buffer, said second solution being contacted with and stored within said second sub-chamber; ande) a chemical analysis mechanism for periodically sampling said second solution and detecting the presence of said pharmaceutically active ingredient of said, pharmaceutical composition therein such that when said first solution is contained within said first sub-chamber, the rate of diffusion by which said pharmaceutically active ingredient of said pharmaceutical composition diffuses across said section of gastrointestinal tissue can be measured at periodic intervals.