Patent ID: 8926947
Filing Date: 2015-01-06
Classification: A61K,B82Y

Abstract:
1. A method of making an autoclavable carboxymethylated reduced dextran iron oxide complex intended for administration by injection to a mammalian subject, the method comprising the steps of: (i) reacting a dextran with a borohydride salt, or hydrogen in the presence of a hydrogenation catalyst, to produce a reduced dextran; (ii) carboxymethylating the reduced dextran to produce a carboxymethylated reduced dextran; (iii) complexing the carboxymethylated reduced dextran with an iron salt to produce a carboxymethylated reduced dextran iron oxide complex; and (iv) sterilizing the carboxymethylated reduced dextran iron oxide complex by autoclaving; the complexing comprising contacting the carboxymethylated reduced dextran with a mixture of ferric and ferrous salts, cooling the resulting solution and adding ammonium hydroxide to neutralize the solution and recovering the carboxymethylated reduced dextran iron oxide complex; such complex being stable at a temperature of at least about 121° C. for a period effective to sterilize the complex; wherein the reduced dextran has an average molecular weight of about 10 kDa and a particle size of 10 nm to 50 nm; and the carboxymethylated reduced dextran comprises at least about 1100 micromoles of carboxyl groups per gram and less than about 1500 micromoles of carboxyl groups per gram.