Patent ID: 7169557
Filing Date: 2007-01-30
Classification: C12Q,Y10S

Abstract:
1. A kit for sequencing a target nucleic acid template, comprising at least one universal nucleotide, at least one polymerase, and at least one specific terminator; wherein one or more of the at least one universal nucleotides is individually selected from 2′-deoxy-7-azaindole-5′-triphosphate (d7AITP), 2′-deoxy-isocarbostyril-5′-triphosphate (dICSTP), 2′-deoxy-propynylisocarbostyril-5′-triphosphate (dPICSTP), 2′-deoxy-6-methyl-7-azaindole-5′-triphosphate (dM7AITP), 2′-deoxy-imidizopyridine-5′-triphosphate (dImPyTp), 2′-deoxy-pyrrollpyrizine-5′-triphosphate (dPPTP), 2′-deoxy-allenyl-7-azaindole-5′-triphosphate (dA7AITP), and 2′-deoxy-propynyl-7-azaindole-5′-triphosphate (dP7AITP).