Patent ID: 7041447
Filing Date: 2006-05-09
Classification: C12Q

Abstract:
1. A method for determining the haplotype structure of a contiguous DNA segment comprising a first nucleotide polymorphism (NP) and a second NP separated by at least 200 nucleotides, said method comprising: (a) obtaining a DNA sample comprising said contiguous DNA segment; (b) using said DNA sample as a template for polymerase chain reaction (PCR) amplification of a DNA fragment comprising said contiguous DNA segment, wherein the PCR amplification is performed with a first primer capable of annealing to a region adjacent to the first NP and distal to the second NP and a second primer capable of annealing to a region adjacent to the second NP and distal to the first NP; (c) ligating the ends of said DNA fragment to each other so as to produce a circular DNA molecule, wherein said first NP and said second NP are brought into closer proximity on said circular DNA molecule relative to said contiguous DNA segment; and (d) determining the haplotype of said first NP and said second NP.