Patent ID: 8367371
Filing Date: 2013-02-05
Classification: C12N,C12P

Abstract:
1. A method for producing an L-amino acid selected from the group consisting of L-lysine and L-threonine, the method comprising cultivating an L-amino acid producing E. coli bacterium in a medium; and collecting the L-amino acid from the medium, wherein the L-amino acid producing E. coli bacterium is modified to enhance glutamate decarboxylase activity by a method selected from the group consisting of: A) transformation of the B) increasing the copy number of a gene selected from the group consisting of gadA and gadB by introduction of the gene into the chromosome of the C) replacing a promoter for a gene selected from the group consisting of gadA and gadB with a stronger promoter, and D) combinations thereof; wherein the gadA gene is a DNA selected from the group consisting of: (a) a DNA comprising the nucleotide sequence of SEQ ID NO: 1; and (b) a DNA that hybridizes with the entire nucleotide sequence complementary to SEQ ID NO: 1 under stringent conditions comprising washing at 68° C. in a salt concentration of 0.1×SSC and 0.1% SDS, and wherein said DNA encodes a protein that has glutamate decarboxylase activity, wherein the gadB gene is a DNA selected from the group consisting of: (c) a DNA comprising the nucleotide sequence of SEQ ID NO: 3; and (d) a DNA that hybridizes with the entire nucleotide sequence complementary to SEQ ID NO: 3 under stringent conditions comprising washing at 68° C. in a salt concentration of 0.1×SSC and 0.1% SDS, and wherein said DNA encodes a protein that has glutamate decarboxylase activity.