Patent ID: 8835611
Filing Date: 2014-09-16
Classification: A61K,A61P,C07K

Abstract:
1. A composition comprising monomeric calicheamicin derivative/anti-CD22 antibody conjugates with reduced low conjugated fraction and having the formula, Pr(—X—W)m, wherein: Pr is an anti-CD22 antibody and comprises SEQ ID NO: 1 for CDR-H1, SEQ ID NO: 2 or SEQ ID NO: 13 or SEQ ID NO: 15 or SEQ ID NO: 16 or residues 50-66 of SEQ ID NO: 23 or residues 50-66 of SEQ ID NO: 27 for CDR-H2, SEQ ID NO: 3 for CDR-H3, SEQ ID NO: 4 for CDR-L1, SEQ ID NO: 5 for CDR-L2 and SEQ ID NO: 6 for CDR-L3; W is a calicheamicin derivative comprising a calicheamicin; X is a hydrolyzable linker that links the anti-CD22 antibody to the calicheamicin derivative and that is capable of releasing the calicheamicin derivative from the calicheamicin derivative/anti-CD22 antibody conjugate after binding and entry into target cells; m is the average loading for a purified conjugation product such that the calicheamicin constitutes 4-10% of the calicheamicin derivative/anti-CD22 antibody conjugate by weight; and wherein the composition has a low conjugated fraction of less than 10%.