Patent ID: 6867013
Filing Date: 2005-03-15
Classification: C12N

Abstract:
1. A method of screening a candidate molecule to identify/determine its ability to inhibit (antagonize) or agonize a recombinant polypeptide encoded by a nucleic acid molecule comprising the sequence of SEQ ID NO:5 or of SEQ ID NO:13, said method comprising the steps of: (a) adding the candidate molecule to a medium which contains the polypeptide; (b) determining the level of kinase activity in the medium; and (c) comparing the level of kinase activity of step (b) with the level of kinase activity that occurs in the medium in the presence of the polypeptide and the absence of the candidate molecule; wherein a decreased level of kinase activity of step (b), as compared to the level of kinase activity that occurs in the medium in the presence of the polypeptide and the absence of the candidate molecule, indicates an antagonist; and an increased level of kinase activity of step (b), as compared to the level of kinase activity that occurs in the medium in the presence of the polypeptide and the absence of the candidate molecule, indicates an agonist.