Patent ID: 9115202
Filing Date: 2015-08-25
Classification: A23J,A23L,C07K

Abstract:
1. A method of forming a canola protein isolate, which comprises: (a) solubilizing a canola protein micellar mass using an aqueous calcium salt solution of a concentration of about 1.5 to about 15 wt % of the protein present to form a canola protein solution having a protein concentration of about 5 to about 50 wt, (b) diluting the canola protein solution by about 1 to about 20 fold, (c) removing deposited phytic acid to provide a clear diluted aqueous canola protein solution, (d) adjusting the pH of the clear diluted aqueous canola protein solution to a value of about 2.0 to about 4.0 to result in an acidified clear canola protein solution, (e) concentrating the acidified clear canola protein solution to a concentration of about 50 to about 200 g/L by using a selective membrane technique to provide a concentrated clear diluted acidified canola protein solution, (f) subjecting the concentrated clear acidified aqueous canola protein solution to diafiltration using about 2 to about 40 volumes of diafiltration solution to provide a concentrated and diafiltered clear diluted acidified canola protein solution, (g) optionally subjecting the concentrated and diafiltered clear diluted acidified canola protein solution to a colour removal step, and (h) drying the concentrated and diafiltered clear diluted acidified protein solution to produce a canola protein isolate having a protein content of at least about 90 wt % (N×6.25) d.b.