Patent ID: 6087491
Filing Date: 2000-07-11
Classification: C07H

Abstract:
A method of manufacturing a N-mer oligonucleotide composition of ultrahigh purity, the method comprisinga) synthesizing the oligonucleotide with dimer blocks using phosphoramidite chemistry, said synthesis comprising contacting a nascent oligonucleotide with 6 or fewer equivalents of a phosphoramidite dimer block, oxidation, detritylation, and repeating each the foregoing steps in this part (a) until the N-mer oligonucleotide is obtained;b) subjecting the full-length, N-mer oligonucleotide synthesized in part (a) to (i) ion-exchange chromatography or to (ii) either reversed phase or hydrophobic interaction chromatography followed by ion-exchange chromatography;c) pooling chromatographic fractions from part (b) having equal to or greater than 99% total purity of N-mer oligonucleotide.