Patent ID: 9175260
Filing Date: 2015-11-03
Classification: A61P,C12N

Abstract:
1. A method of differentiating primate Pluripotent Stem Cells (pPSCs) into Isl1+ mesoderm cells comprising (a) providing pPSCs; (b) contacting the pPSCs with an effective amount of at least one GSK inhibitor selected from the group consisting of a GSK3 inhibitor and a Wnt protein in a cell differentiation medium for a period sufficient to produce mesendoderm cells; and (c) subsequently contacting the cells obtained from step (b) with an effective amount of a bone morphogenic protein (BMP) and optionally, a GSK inhibitor selected from the group consisting of a GSK3 inhibitor and a Wnt protein for a period sufficient to produce said mesoderm cells; and (d) optionally, isolating said mesoderm cells.