Patent ID: 6171856
Filing Date: 2001-01-09
Classification: A61K,C12N,Y10S

Abstract:
A method of generating a mammalian cell line that is resistant to IL-1.beta. mediated immunotoxicity comprising:(a) providing a mammalian cell;(b) introducing in vitro an MnSOD (maganese superoxide dismutase) protein encoding gene operatively linked to a first promoter into said mammalian cell;(c) selecting a cell from step (b) that exhibits an increased level of MnSOD activity as compared to the mammalian cell of step (a); and(d) propagating said selected cell into a cell line,wherein increased level of MnSOD activity provides said cell line with resistance to IL-1.beta. mediated immunotoxicity by inhibiting iNOS (inducible form of nitric synthase) and blocking NO (nitric oxide) production in said cell line.