Patent ID: 6207459
Filing Date: 2001-03-27
Classification: G01N

Abstract:
A method for the determination of the concentration of an analyte in a sample containing free haemoglobin by optical measurement wherein the measured value for the analyte concentration is corrected by the steps:a. optically measuring a blank value of the sample to be analyzed,b. optically measuring a blank value of a haemoglobin-free reference sample,c. adding a reagent to the sample to produce a detectable change in the sample indicative of analyte concentration,d. optically measuring an uncorrected value for the analyte concentration ande. correcting the uncorrected value obtained in step (d) by correlation with the blank values obtained in steps (a) and (b) in order to obtain a corrected value for the analyte concentration,wherein the corrected value for the analyte concentration is calculated according to the following relation:C'.sub.sample =C.sub.sample -F.E1.sub.sample +F.E1.sub.referencein which C'.sub.sample is the corrected value for the analyte concentration,C.sub.sample is the uncorrected measured value for the analyte concentration in the sample,F is a test-specific correction factor,E1.sub.sample is the measured blank value in the sample and E1.sub.reference is the measured value in the reference sample.