Patent ID: 6255060
Filing Date: 2001-07-03
Classification: C12Q,G01N

Abstract:
A method of detecting a phosphorylated tau protein by immuno-aRNA, wherein the presence of the phosphorylated tau protein is correlated with Alzheimer's disease comprising:a) immobilizing a first antibody targeted to a phosphorylated tau protein to a solid support;b) contacting the solid support with the protein so that the phosphorylated tau protein binds to the immobilized first antibody;c) contacting the solid support with a RNA promoter-driven cDNA sequence covalently coupled to a second antibody targeted to the phosphorylated tau protein so that the second antibody binds to the bound phosphorylated tau protein on the solid support; andd) detecting the promoter-driven cDNA sequence covalently coupled to the bound second antibody by amplified RNA techniques, wherein the presence of the promoter-driven cDNA is indicative of the presence of the phosphorylated tau protein in the cell, said protein correlating with Alzheimer's disease.