Patent ID: 6218168
Filing Date: 2001-04-17
Classification: C12N,C12P

Abstract:
A recombinant serine acetyltransferase which exhibits a sensitivity to the inhibitor L-cysteine which is reduced in comparison to the wild-type enzyme and whose protein sequence exhibits at least one mutation or deletion when compared with the wild-type sequence,wherein the mutation is in the sequence region of amino acids 97-100, 164-169, 237, 239-240, 245-259 and 267-269 or the deletion is in the carboxyterminal sequence region of amino acids 237-240, 245-259 and 267-269,with position 1 being the start methionine in SEQ ID NO: 1 and with the mutation of Met to Ile in position 256 being excluded, and wherein the serine acetyltransferase has an inhibitor constant, K.sub.i, of from 0.02 to 2.3 mM in the presence of 1 mM L-serine and 0.1 mM acetyl-CoA.