Patent ID: 8456158
Filing Date: 2013-06-04
Classification: B03C,G01N

Abstract:
1. A detecting method comprising the steps of: preparing a magnetic property imparted binding substance, which is a first binding substance that specifically binds with a detection target substance, having magnet enveloping dielectric particles, which have magnetic particles enveloped therein and the surfaces of which are modified with functional groups that exhibit polarity within a liquid sample, attached thereto; preparing a labeling binding substance, which is one of a second binding substance that specifically binds with the detection target substance and a third binding substance that specifically binds with the first binding substance in competition with the detection target substance, having photoresponsive labels attached thereto; mixing the liquid sample, which is a target of inspection, the magnetic property imparted binding substance, and the labeling binding substance, to cause binding reactions to occur; generating a magnetic field within a sample cell that contains the liquid sample, in which the magnetic property imparted binding substance and the labeling binding substance are mixed, to draw the magnetic property imparted binding substance to a local region within the sample cell; irradiating excitation light only onto a predetermined region that includes the local region, in a state in which the magnetic property imparted binding substance is drawn to the local region, to cause optical signals to be generated by the photoresponsive labels which are present within the predetermined region; detecting the optical signals; and determining the amount of the detection target substance within the liquid sample, based on the amount of detected optical signals, wherein: the maximum length of the combinations of the magnetic property imparted binding substance, the detection target substance, and the labeling binding substance, which is the second binding substance having the photoresponsive labels attached thereto, is greater than or equal to 200 nm.