Patent ID: 8247179
Filing Date: 2012-08-21
Classification: C12Q,G01N

Abstract:
1. A process for detecting qualitatively or quantitatively the presence of a single-stranded or double-stranded nucleic acid of interest in a sample, said process comprising the steps of: (a) providing (b) forming a reaction mixture comprising (i), (ii) and (iii) above; (c) contacting under hybridization conditions said first nucleic acid primer with one strand of said nucleic acid of interest and contacting under hybridization conditions said second nucleic acid primer with the complementary strand of said nucleic acid of interest if present; (d) extending said first nucleic acid primer and said second nucleic acid primer to form a first primer-extended nucleic acid sequence and a second primer-extended nucleic acid sequence if said complementary strand is present; (e) separating said first primer-extended nucleic acid sequence from said nucleic acid of interest and separating said second primer-extended nucleic acid sequence from said complementary strand of said nucleic acid of interest if present; (f) contacting under hybridization conditions said first nucleic acid primer with said nucleic acid of interest or said second primer-extended nucleic acid sequence from step (e), and contacting under hybridization conditions said second nucleic acid primer with said first primer-extended nucleic acid sequence from step (e); and (g) detecting the presence or quantity of said nucleic acid of interest by detecting energy transfer between said first and second energy transfer elements.