Patent ID: 8492156
Filing Date: 2013-07-23
Classification: G01N,G06K,G06T

Abstract:
1. A method of staining a sample for microscopy imaging whereby the image of the stained sample is configured to exhibit an optimum contrast between sub-cellular components for diagnosis by a pathologist, said method comprising: staining a sample with a dye; determining a transmittance value of the dye from a microscopy image of the sample; forming an artificial image of the sample from the determined transmittance value of the dye; varying the transmittance value of the dye, by applying one of an absolute weighting coefficient and a relative weighting coefficient to an optical density corresponding thereto, so as to form a series of artificial images each demonstrating a different transmittance value of the dye; selecting one image, from the series of images, exhibiting the optimum contrast between sub-cellular components for the dye and determining the corresponding transmittance value of the dye in the one image; and varying staining of the sample with the dye so as to provide a stained sample having the transmittance value of the dye corresponding to the optimum contrast between sub-cellular components.