Patent ID: 7264932
Filing Date: 2007-09-04
Classification: C07K,C12N,C12Q,G01N

Abstract:
1. A method comprising: a) obtaining at least a first nuclease inhibitor, wherein the first nuclease inhibitor is a RNase inhibitor protein or an anti-nuclease antibody; b) obtaining at least a second nuclease inhibitor wherein the second nuclease inhibitor is oligovinylsulfonic acid (OVA), aurintricarboxylic acid (ATA), aflatoxin, 2-nitro-5-thiocyanobenzoic acid, iodoacetate, N-bromosuccinimide, p-chloromercuribenzoate, dinitrofluorobenzene, decanavanate, polyvinylsufonic acid, hydrobenzoinphosphate, phenyiphosphate, putrescine, haloacetate, dinitrofluorobenzene, phenyiglyoxal, bromopyruvic, 8-amino-5-(4′-hydroxy-biphenyl-4-ylazo)-naphthalene-2-sulfonate, 6-hydroxy-5-(2-hydroxy-3,5-dinitro-phenylazo)-naphthalene-2-sulfonate, 3,3′-dimethylbiphenyl-4,4′-bis(2-amino-naphthylazo-6-sulfonate), 4,4′-dicarboxy-3,3-bis(naphthylamido)-diphenylmethanone, 3,3′-dicarboxy-4,4′-bis(4-biphenylamido)diphenylmethane, 3,3′-dicarboxy-4,4′-bis(3-nitrophenylamido)diphenylmethane or NCI-224131; c) obtaining a composition; and d) admixing the first nuclease inhibitor, the second nuclease inhibitor and the composition to form an admixture: wherein nucleases that may be present in the admixture are inhibited.