Patent ID: 6573427
Filing Date: 2003-06-03
Classification: C12N

Abstract:
A nucleic acid molecule comprising 5â€² to 3â€²:(a) a transcriptional promoter; (b) a viral replicase gene; (c) at least one polypeptide encoding nucleic acid sequence; (d) a subgenomic promoter sequence, which comprises a transcription initiation site, operatively linked to the polypeptide encoding nucleic acid sequence to be expressed; such that the replicase gene, the polypeptide encoding nucleic acid sequence and the subgenomic promoter sequence are transcribed under the action of said transcriptional promoter to give a primary transcript which is transcribed under the action of the replicase expressed from said viral replicase gene and said subgenomic promoter to give a secondary transcript; wherein the replicase gene is in an orientation which on transcription gives a primary RNA transcript encoding for said replicase gene in a positive sense orientation; and wherein the polypeptide encoding nucleic acid sequence to be expressed and the subgenomic promoter sequence are in an orientation which on transcription gives a primary RNA transcript encoding said polypeptide encoding nucleic acid and said subgenomic promoter in a negative sense orientation.