Patent ID: 6203988
Filing Date: 2001-03-20
Classification: C12Q

Abstract:
A DNA fragment preparation method for gene expression profiling, comprising the steps of:i) preparing a plurality of DNA fragments including a sequence-known DNA fragment and a sequence-unknown DNA fragment from a sample DNA; andii) amplifying fragments each having a shorter length than the length of the sequence-known DNA fragment and the sequence-unknown DNA fragment by polymerase chain reaction (PCR), using the DNA fragments as templates and using first and second primers, each of which hybridizes with a terminus sequence of each of the DNA fragments, and a specific primer which hybridizes specifically with a base sequence of the sequence-known DNA fragment at a position between a priming site of the first primer and a priming site of the second primer,wherein, by using the first primer, the second primer and the specific primer at the same time, and by competitive reactions occurring between PCR by the first primer and the second primer and PCR by the second primer and the specific primer, the fragments each having a part of the base sequence of the sequence-known DNA fragment and having a shorter length than the lengths of the PCR products by the first primer and the second primer are produced as major products in the PCR by the second primer and the specific primer.