Patent ID: 8592163
Filing Date: 2013-11-26
Classification: C07K,C12N

Abstract:
1. A method of determining immune enhancement of virus infectivity comprising: exposing a first virion-antibody mixture comprising virion-antibody complexes to a first set of cells, wherein the cells do not express a native Fc receptor but comprise a bicistronic DNA construct that expresses both an Fc receptor γ-chain polypeptide and an Fc receptor α-subunit, with the Fc receptor γ-chain polypeptide being expressed in stoichiometric excess of the Fc receptor α-subunit, whereby the recombinant cells comprise a recombinant Fc receptor competent for both binding and uptake of virion-antibody complexes; measuring infectivity of the first virion-antibody mixture in the first set of cells; providing an infectivity standard; and comparing the measured infectivity to the infectivity standard, wherein an increase in infectivity relative to the infectivity standard indicates that antibodies in the first virion-antibody mixture promote immune enhancement.