Patent ID: 6495326
Filing Date: 2002-12-17
Classification: C12Q

Abstract:
A method for analyzing data obtained by a method for analyzing or determining a polymorphism or a mutation in a target nucleic acid or gene, which comprises:correcting a fluorescence intensity of a reaction system, in which said target nucleic acid or gene has been hybridized with a nucleic acid probe labeled with a fluorescent dye, in accordance with a fluorescence intensity of said reaction system before said hybridization; wherein the method for analyzing or determining polymorphism or mutation of a target nucleic acid or gene comprises: hybridizing the nucleic acid probe to said target nucleic acid or gene, wherein only said nucleic acid probe is provided for one type of target nucleic acid, and measuring a change in fluorescence; wherein said probe is labeled at an end portion thereof with said fluorescent dye, and said probe has a base sequence designed such that, when said probe is hybridized with said target nucleic acid, at least one guanine base exists in a base sequence of said target nucleic acid at a position 1 to 3 bases from an end base portion where said probe and said target nucleic acid are hybridized with each other; whereby said fluorescent dye is reduced in fluorescence emission when said probe is hybridized with said target nucleic acid.