Patent ID: 9040262
Filing Date: 2015-05-26
Classification: A61K,C07K,C12N,C12P,C12Y

Abstract:
1. A method for preparing compound of Formula (Ia) in diastereomeric excess wherein, X is C or S; R R R R R said method comprising: contacting a compound of Formula (IIa), wherein, X, R with a non-naturally occurring ketoreductase polypeptide in the presence of cofactor NADPH or NADH under suitable reaction conditions, wherein the polypeptide comprises an amino acid sequence having at least 80% sequence identity to SEQ ID NO:2 and the following amino acid residues: R at position X40; I or L at position X153; A, or P at position X190; A, C, N, S, or T at position X196; F or W at position X199; and I at position X206.