Patent ID: 6103509
Filing Date: 2000-08-15
Classification: C12N

Abstract:
A pyrrolo-quinoline quinone glucose dehydrogenase (PQQGDH) enzyme comprising the amino acid sequence Ala-Gly-Gly-Xaa-Gly-Ser-Phe (SEQ ID No:11), wherein said Xaa residue is any amino acid residue other than histidine, wherein said SEQ ID No:11 is found in a region corresponding to the sequence Ala-Gly-Gly-His-Gly-Ser-Phe (SEQ ID No:10) found in wild-type E. coli PQQGDH, and wherein said PQQGDH enzyme has increased substrate specificity to glucose compared to that of said wild-type E. coli PQQGDH that comprises SEQ ID No:10.