Patent ID: 8735060
Filing Date: 2014-05-27
Classification: C12N

Abstract:
1. A method for purifying RNA from a biological sample comprising at least double stranded DNA and single-stranded RNA, said method comprising contacting the sample comprising said DNA and said RNA with a lysis buffer, nanocarrier beads and magnetizable beads under conditions suitable for formation of a complex comprising said nanocarrier beads, said DNA and said magnetizable beads, thereby forming a first mixture comprising said complex, wherein said lysis buffer: is configured to provide said conditions suitable for formation, upon said contacting the sample comprising said DNA and said RNA with said lysis buffer, said nanocarrier beads and said magnetizable beads, of said complex comprising said nanocarrier beads, said DNA and said magnetizable beads, wherein, under said conditions, said DNA binds said nanocarrier beads and said magnetizable beads and wherein said complex does not comprise the RNA; and comprises an aqueous salt solution with a salt concentration of greater than 1M, applying a magnetic field to said first mixture, separating and removing said complex comprising said nanocarrier beads, said DNA and said magnetizable beads from the said first mixture via the magnetic field and forming a second mixture, subsequently adding a binding buffer to said second mixture, and purifying said RNA from said second mixture and obtaining purified RNA.