Patent ID: 7230157
Filing Date: 2007-06-12
Classification: C12N

Abstract:
1. A method for screening for conditional dominant-positive or gain-of-function mutations in a gene of a plant or a plant cell, said method comprising a) transforming a plant or a plant cell with a nucleic acid comprising (i) a DNA construct encoding a transcription factor and having the following operably linked elements in the 5′ to 3′ direction: (a) a first promoter, (b) DNA encoding a DNA-binding domain selected from the group consisting of a bacterial repressor LexA DNA-binding domain and a GAL4 DNA-binding domain, (c) DNA encoding a transactivating domain of VP16, and (d) DNA encoding a regulatory domain selected from the group consisting of an estrogen receptor regulatory domain and a glucocorticoid receptor regulatory domain and (ii) a DNA construct comprising as operably linked elements (a) an operator selected from the group comprising a GAL4 operator and a LexA operator and (b) a second promoter; to produce a transformed plant or plant cell; b) growing said transformed plant or plant cell under selective conditions; and c) recovering mutants capable of growth under the selective conditions.