Patent ID: 9110056
Filing Date: 2015-08-18
Classification: G01N

Abstract:
1. A biosensor comprising a flat surface encompassed by a homogeneous area comprising a monolayer of molecules with no diffusive seed molecules that can relocate and destroy amplification accuracy, the monolayer of molecules produced on the surface via a method comprising: loading a stamp with seed molecules; transferring seed molecules from the stamp to the flat surface, wherein the transferring comprises transferring a fraction of the seed molecules loaded on the stamp to the flat surface and wherein the transferring comprises adsorbing the seed molecules to the stamp and adsorbing the seed molecules to the flat surface, the adsorption of the seed molecules to the stamp being stronger than the adsorption of the seed molecules to the flat surface; and self-completing amplification of the seed molecules via an amplifying reaction to produce the monolayer on the flat surface, wherein self-completing amplification of the seed molecules via an amplifying reaction to produce the monolayer comprises producing a homogeneous area extending over the entirety of the flat surface, wherein the homogeneous area comprises a monolayer of molecules formed from a single type of molecule.