Patent ID: 7344735
Filing Date: 2008-03-18
Classification: C12N

Abstract:
1. A method of counting protozoal sporozoites in a mammalian tissue sample comprising protozoal sporocysts, comprising: a) exposing said mammalian tissue sample to a sodium hypochlorite solution having a sodium hypochlorite concentration in a range of about 15-25% v/v for a period of up to about 45 minutes; b) removing said sodium hypochlorite from said mammalian tissue sample by rinsing said mammalian tissue sample with a saline solution; c) exposing said rinsed mammalian tissue sample from step (b) to an excystation fluid to affect excystation of said protozoal sporocysts to release sporozoites, wherein said excystation takes place with substantially no incubation or washing; and d) counting said sporozoites.