Patent ID: 8772049
Filing Date: 2014-07-08
Classification: B01J,B01L,B82Y,G01N,Y10S,Y10T

Abstract:
1. A method of screening a monoclonal antibody comprising: (a) reversibly sealing a moldable slab to a substrate, said moldable slab comprising an array of microwells, each microwell having a side or diameter of less than 100 microns, wherein microwells of said array contain a single or a few antibody-producing cell(s) that secrete monoclonal antibodies in a volume of 10 nanoliters or less of fluid in said microwells; wherein the substrate's surface facing the microwells is modified such that it acts to retain such secreted monoclonal antibodies on the surface; (b) allowing said antibody-producing cell(s) to secrete said monoclonal antibodies in said volume such that monoclonal antibody secreted from the antibody-producing cell(s) in said volume is retained by the surface of the substrate sealed to the moldable slab; (c) removing the substrate from the moldable slab; and (d) exposing the retained monoclonal antibody to an antigen to test the retained monoclonal antibody for a desired binding specificity.