Patent ID: 6030775
Filing Date: 2000-02-29
Classification: C12Q

Abstract:
A method for testing a tissue sample to determine the allelic type of an HLA Class I gene in the sample, said HLA Class I gene being selected from among HLA-A, HLA-B and HLA-C genes comprising the steps of(a) treating the tissue sample to obtain nucleic acid polymers suitable for amplification;(b) combining the nucleic acid polymers with a first primer which hybridizes with a portion of intron 1 or intron 3 of the HLA Class I gene, and a second primer which hybridizes with a different portion of the HLA Class I gene conditions suitable for amplification to obtain an amplified product, wherein the first primer and the second primer flank a region including at least one site of allelic variation in at least one of exons 2 or 3 of the HLA Class I gene and wherein the first primer is a locus specific primer which hybridizes with intron 1 or intron 3 of only one of the HLA Class I genes; and(c) evaluating the amplified product to determine the allelic type of the HLA-Class I gene.