Patent ID: 8043804
Filing Date: 2011-10-25
Classification: A61P,C07K,C12N,G01N

Abstract:
1. A method for identifying an agent which inhibits the complexation between DBC1 and SIRT1 comprising: (a) providing a first composition comprising an amount of DBC1 bound to SIRT1 and a control composition comprising the same amount of DBC1 bound to SIRT1 as the first composition; (b) contacting the first composition with a test agent; (c) determining i) the level of unbound SIRT in the first composition and the control composition or ii) the level of complexation between SIRT1 and DBC1 in the first composition and the control composition; and (d) determining i) the difference in the level of unbound SIRT1 in the first composition compared to the control composition or ii) the difference in the level of complexation between SIRT1 and DBC1 in the first composition compared to the control composition; wherein, if i) the level of unbound SIRT1 in the first composition is higher than the level of unbound SIRT1 in the control composition or ii) the level of complexation between SIRT1 and DBC1 in the first composition is lower than the level of complexation between SIRT1 and DBC1 in the control composition, then the test agent inhibits the complexation between SIRT1 and DBC1.