Patent ID: 8916356
Filing Date: 2014-12-23
Classification: C12Q,G01N

Abstract:
1. A method for in vitro determining thrombin activity in a biological sample which is a sample of whole blood or a plasma sample and wherein thrombin generation is measured by the steps of: contacting a layer of said sample with a fluorogenic substrate of thrombin, wherein said layer has a thickness within a range of about 0.05 to about 5 mm and a surface within a range of about 10 to 500 mm allowing thrombin to generate in said sample, wherein said filter grid or packed spheres maintains the homogeneous dispersion of the sample after thrombin activation while reducing sedimentation of the sample and retraction of the clot within the assayed sample; and measuring the fluorescence emitted from the surface of the layer, by the fluorescent group released from the fluorogenic substrate as a result of enzymatic action of generated thrombin on said fluorogenic substrate.