Patent ID: 7172864
Filing Date: 2007-02-06
Classification: B01J,B01L,B82Y,C07B,C07H,C07K,C12Q,C40B,G11C,H01L

Abstract:
1. A method for an electronically controlled enzymatic reaction at an addressable location, comprising the steps of: providing an array of microlocations comprising a permeation layer coupled to a plurality of electrodes, wherein each microlocation comprises a distinct electrode within the plurality of electrodes; contacting a biomolecule in solution with the permeation layer at a microlocation; concentrating the biomolecule at the microlocation by placing the electrode of the microlocation at an opposite charge to the biomolecule; attaching the biomolecule to the permeation layer at the microlocation; and reacting an enzyme with the biomolecule at the microlocation, wherein the enzyme is selected from the group consisting of a restriction enzyme, a ligase, a proteinase, a glycosidase, a DNA polymerase, a RNA polymerase, and a phosphorylase.