Patent ID: 6030815
Filing Date: 2000-02-29
Classification: C07H,C12P,Y02P

Abstract:
A method for sialylating an acceptor sugar, comprising:(a) providing a reaction medium comprising:(i) a sialyl transferase;(ii) a catalytic amount of a CMP-sialic acid synthetase;(iii) a sialic acid;(iv) an acceptor sugar for said sialyltransferase;(v) CTP; and(vi) a soluble divalent metal cation; and(b) adding sufficient divalent metal cation to said reaction medium to restore a portion of said divalent cation lost during the course of the reaction to thereby achieve or maintain a concentration of said divalent metal cation in said reaction medium between about 1 mM and about 75 mM, and wherein step (b) occurs without interruption of said sialylating reaction.