Patent ID: 8846578
Filing Date: 2014-09-30
Classification: A61K,A61P,C07K,C12N

Abstract:
1. A method of promoting recombination within a CTFR gene in a human cell comprising contacting said cell with a first zinc three-finger binding domain that targets a nucleotide sequence TTAGAAGTG (SEQ ID NO:132) and a second zinc three-finger binding domain that targets a nucleotide sequence TTATGGGAG (SEQ ID NO:133), wherein each of said first and second zinc three-finger binding domains are linked to a non-specific nuclease, wherein said first zinc three-finger binding domain comprises a sequence selected from SEQ ID NOS:3-5, 6-8, 9-11, 12-14, 15-17, 18-20, 21-23, 24-26, 27-29, 30-32, 33-35 and 36-38 for TTAGAAGTG (SEQ ID NO:132) and said second zinc three-finger binding domain comprises a sequence selected from the group consisting of SEQ ID NOS:39-41, 42-44, 45-47, 48-50, 51-53, 54-56, 57-59, 60-62, 63-65, 66-68, 69-71, 72-74, 75-77, 78-80, 81-83, 84-86, 87-89, 90-92, 91-93, 94-96, 97-99, 100-102, 103-105, 106-108, and 109-111 for TTATGGGAG (SEQ ID NO:133).