Patent ID: 7037684
Filing Date: 2006-05-02
Classification: C07K,C12N,C12P

Abstract:
1. A DNA which enables co-expression of a protein disulfide isomerase and a polypeptide having disulfide bonds, comprising at least one promoter required for gene expression, two identical or different Shine-Dalgamo sequences (SD), two identical or different sequences encoding signal peptides of cell wall proteins (CWP) of a bacterium of the genus Bacillus (CWPsp), a gene encoding a polypeptide having disulfide bonds which is a fusion protein comprising human insulin, and a gene encoding protein disulfide isomerase (PDI) that are ligated to each other as represented by the following formula: wherein X wherein the fusion protein comprising a human insulin is represented by the following formula: MWPmp9-GSLQPR-B chain-RGHRP-Linker-PR-A chain, wherein MWPmp9 represents 9 amino acids from the N terminus of a middle-wall mature protein of bacteria of the genus