Patent ID: 6436675
Filing Date: 2002-08-20
Classification: B01J,C12N

Abstract:
A method of providing a population of recombined nucleic acids, the method comprising:(i) providing a population of oligonucleotides comprising at least one set of codon-varied oligonucleotides, wherein at least one member of the set of codon-varied oligonucleotides is chemically synthesized using at least trinucleotide sequences and wherein two or more members of the population comprise overlapping oligonucleotides; (ii) hybridizing at least two of the overlapping oligonucleotides to each other to provide a population of hybridized overlapping oligonucleotides, which population of hybridized overlapping oligonucleotides comprises at least one codon-varied oligonucleotide; and, (iii) elongating members of the population of hybridized overlapping oligonucleotides, thereby providing the population of recombined nucleic acids.