Patent ID: 6235492
Filing Date: 2001-05-22
Classification: A61P,C12N,G01N

Abstract:
A method of screening for an agent which modulates the interaction of an IKK polypeptide to a binding target, said method comprising the steps of:incubating a mixture comprising:an isolated polypeptide comprising at least 10 consecutive residues of the amino acid sequence set forth as SEQ ID NO:4, which consecutive amino acid residues comprise at least one of the amino acid residues 679, 680, 684, 686 and 687 of SEQ ID NO:4,a binding target of said polypeptide, anda candidate agent;under conditions whereby, but for the presence of said agent, said polypeptide specifically binds said binding target at a reference affinity;detecting the binding affinity of said polypeptide to said binding target to determine an agent-biased affinity, wherein a difference between the agent-biased affinity and the reference affinity indicates that said agent modulates the binding of said polypeptide to said binding target.