Patent ID: 6435012
Filing Date: 2002-08-20
Classification: B01D,B01J,G01N

Abstract:
A method for measuring chromatographic media parameters in a packed bed of hydrophobic polymer particles comprising:(a) packing a column with hydrophobic polymer particles and a first solvent to provide a packed bed; (b) introducing a large-molecule probe mixture, comprising a crosslinked ionically-charged polymer particle probe and a second solvent, onto the packed bed; (c) eluting the crosslinked ionically-charged polymer particle probe from the packed bed by passing a third solvent through the packed bed, wherein the third solvent is selected from one or more of polar organic solvent and water; and (d) determining an elution volume for the crosslinked ionically-charged polymer particle probe; wherein the chromatographic media parameter is excluded volume, corresponding to the elution volume for the crosslinked ionically-charged polymer particle probe.