Patent ID: 6150176
Filing Date: 2000-11-21
Classification: C07K,G01N,Y10S

Abstract:
A functional engineered fluorescent protein whose amino acid sequence comprises an amino acid sequence of Aequorea green fluorescent protein (SEQ ID NO: 2) containing an amino acid substitution of histidine at position 148 of SEQ ID NO: 2 for glycine or glutamine and amino acid substitutions selected from the group consisting of:F64L/S65T/H231L of SEQ ID NO:2;S65G/S72A/T203Y/H231 L of SEQ ID NO: 2;S65G/V68L/Q69K/S72A/T203Y/H231 L of SEQ ID NO: 2;K26R/F64L/S65T/Y66W/N1461/M153T/V163A/N164H/H231L of SEQ ID NO: 2; andS65G/V68L/S72A/Q80R/T203Y of SEQ ID NO:2,and whose emission intensity changes as pH varies between 5 and 10.