Patent ID: 7247483
Filing Date: 2007-07-24
Classification: B01L,G01F,G01N,Y10T

Abstract:
1. A method for determining the volume of a dispensed liquid sample, wherein the sample liquid comprises a fixed concentration of indicator ions comprising the steps of: providing a diluent in a container, the diluent comprising polydentate chromogenic ligands of one type; dispensing a volume of the sample liquid into the diluent, thereby forming complexes of the polydentate chromogenic ligands with the indicator ions, which complexes are chromophoric indicators for staining the resulting mixture; measuring the optical absorption of the stained mixture in the container; and determining the volume of the dispensed liquid sample by correlating the measured optical absorption of the stained mixture with the optical absorption of a test sample that has an exactly defined concentration of the same chromophoric indicator, and wherein the chromophoric indicator is a colored complex that has a three-dimensional coordination geometry, which greatly hinders adsorption of this type of molecule to apolar surfaces.