Patent ID: 8709727
Filing Date: 2014-04-29
Classification: C12Q,G01N

Abstract:
1. A method for quantifying and/or detecting a target molecule, comprising the steps of: forming a target molecule-dependent volume-amplified entity comprising i) a target molecule bound to a passive particle functionalized with a secondary capturing molecule, ii) a Rolling Circle Amplified (RCA) product formed through a target-dependent DNA circularization reaction, or iii) a RCA product formed through a target-dependent DNA circularization reaction and bound to a passive particle functionalized with a secondary capturing molecule; allowing a magnetic nanoparticle, functionalized with a primary capturing molecule, to bind said volume-amplified entity via said primary capturing molecule; and quantifying and/or detecting the target by measuring the change in dynamic magnetic response of the magnetic nanoparticle caused by the increase in hydrodynamic volume of said magnetic nanoparticle, wherein the increase in hydrodynamic volume of the magnetic nanoparticle results from binding with the volume-amplified entity.