Patent ID: 8962273
Filing Date: 2015-02-24
Classification: C07K,C12N,C12P,C12Y

Abstract:
1. A method of producing β-glucocerebrosidase or a β-glucocerebrosidase variant wherein the β-glucocerebrosidase variant is at least 90% identical to SEQ ID NO: 11 and has β-glucocerebrosidase activity, comprising culturing a lysosomal integral membrane protein II (LIMP-2) deficient cell which expresses β-glucocerebrosidase or the β-glucocerebrosidase variant under conditions in which β-glucocerebrosidase or the β-glucocerebrosidase variant is produced, thereby producing β-glucocerebrosidase or the β-glucocerebrosidase variant, wherein the β-glucocerebrosidase or β-glucocerebrosidase variant is secreted from the cell in increased amount compared to LIMP-2 positive cells.