Patent ID: 9102943
Filing Date: 2015-08-11
Classification: A61P,C12N

Abstract:
1. A method of producing a retrovirus particle, which comprises a step of culturing a producer cell line which produces the retrovirus particle and producing the retrovirus particle in a culture medium containing retinoic acid and a histone deacetylase inhibitor as active ingredients and a step of harvesting a supernatant containing the retrovirus particle from the culture medium, wherein the culture medium contains either 100 nM to 3 μM trichostatin A or 1 mM to 10 mM sodium butyrate as the histone deacetylase inhibitor, and wherein the method provides retrovirus particles having higher gene transduction efficiency as compared to culture conditions not comprising retinoic acid and a histone deacetylase inhibitor.