Patent ID: 7619138
Filing Date: 2009-11-17
Classification: A01H,A23L,C07K,C12N

Abstract:
1. A method of transforming a plant cell with a desired polynucleotide, comprising (A) contacting a plant cell with an Agrobacterium strain that comprises a first plasmid, wherein the first plasmid comprises (i) nucleic acid sequences obtained from at least one plant genome that facilitate enzymatic nicking of the plasmid in Agrobacterium , wherein a nucleic acid sequence that facilitates enzymatic nicking comprises a 5′ YGRYAGGATATATWSNVBKGTAAWY-3′ (SEQ ID NO: 47), consensus sequence, wherein Y is C or T; R is A or G; K is C or T; M is A or C; W is A or T; S is C or G; V is A, C, or G; and B is C, G, or T, and (ii) a desired polynucleotide, wherein any plant nucleic acid that facilitates enzymatic nicking of the plasmid is not an Agrobacterium T-DNA or an Agrobacterium T-DNA border sequence, and (B) obtaining a transformed plant cell that comprises the desired polynucleotide in its genome.