Patent ID: 6017701
Filing Date: 2000-01-25
Classification: C12Q

Abstract:
A method for preferentially replicating one or more desired nucleic acids comprising:preparing tester nucleic acids and driver nucleic acids from selected populations of nucleic acids;producing adaptor:driver nucleic acid complexes with said driver nucleic acids, wherein each adaptor:tester nucleic acid complex comprises a tester nucleic acid and a first selected adaptor, wherein the first selected adaptor comprises a priming site;producing adaptor:driver nucleic acid complexes with said driver nucleic acids, wherein each adaptor:driver nucleic acid complex comprises a driver nucleic acid and a second selected adaptor, and said first and second adaptors being the same or different;combining said adaptor:tester nucleic acid complexes and said adaptor:driver nucleic acid complexes so as to produce double-stranded hybrid nucleic acids comprising double-stranded adaptor:tester nucleic acid complexes, double-stranded adaptor:driver nucleic acid complexes, and double-stranded adaptor:tester/adaptor:tester nucleic acid complexes;adding a reagent capable of interacting with said first or second selected adaptor substantially prevent replication of all but double-stranded adaptor:tester nucleic acid complexes; andreplicating said double-stranded adaptor:tester nucleic acid complexes.