Patent ID: 8227256
Filing Date: 2012-07-24
Classification: G01N,G02B

Abstract:
1. A method for optical excitation of a sample via a two color (two-photon) absorption process comprising: (a) exciting the sample with a first wavelength of light and a second wavelength of light; (b) generating the first wavelength of light and the second wavelength of light from a single light source subjected to a Raman shifter, wherein the single light source is a laser beam, wherein the laser beam is adjusted by a laser polarizer and a diaphragm; (c) collimating an output from the Raman shifter with a lens, a first dichroic mirror associated with a first beam dump, and a second dichroic mirror associated with a second beam dump; (d) dispersing the output from the Raman shifter though a first Pellin-Broca prism and a second Pellin-Broca prism resulting in a first confocal excitation beam having the first wavelength of light and a second confocal excitation beam having the second wavelength of light; (e) directing the first confocal excitation beam and the second confocal excitation beam to the sample by mirrors; and (f) detecting an optical property change from the sample.