Patent ID: 6291159
Filing Date: 2001-09-18
Classification: A61K,C07K

Abstract:
A method of producing an isolated expression vector capable of expressing a receptor polypeptide of predetermined specificity, which method comprises the steps:(a) synthesizing a receptor-coding gene library containing a plurality of different receptor-coding DNA sequences by a method comprising the steps:(i) preparing a polynucleotide containing composition, wherein at least a portion of the polynucleotides in said composition comprise a plurality of different receptor-coding sequences(ii) amplifying a plurality of receptor-coding sequences in said polynucleotide containing composition, using amplification primers(iii) joining in operable combination receptor-coding sequences from said receptor-coding gene library into expression vectors so as to be able to express a receptor-coding sequence, whereby a diverse library is formed,(b) selecting from said diverse library at least one expression vector capable of producing receptor polypeptides having the desired specificity.