Patent ID: 6582938
Filing Date: 2003-06-24
Classification: C12Q

Abstract:
A method for the amplification of nucleic acid, said method comprising:contacting mRNA having a poly d(A) tail with a primer consisting of SEQ ID No. 2; generating a first cDNA strand from the mRNA strand by extending the primer by reverse transcriptase and the appropriate nucleotides under the appropriate conditions, which creates a RNA:DNA duplex; denaturing the RNA:DNA duplex; forming a double stranded DNA; adding a promoter to the DNA by hybridizing an oligonucleotide consisting of SEQ ID No. 4 to the single stranded DNA, wherein the oligonucleotide is blocked from extension at the 3â€² end; forming a double stranded DNA promoter region by adding the appropriate reagents; and, producing multiple copies of RNA from the DNA strand comprising the promoter.