Patent ID: 6197502
Filing Date: 2001-03-06
Classification: C12N,C12Q,Y10S

Abstract:
A method for identifying a recombinant nucleic acid encoding an exogenous polypeptide which shares a property of interest with members of a class of polypeptides, said method comprising:a. providing a composition comprising a population of eukaryotic host cells which contain individual expression systems, wherein each host cell comprises at least one copy of one unique expression system comprising said recombinant nucleic acid, said recombinant nucleic acid being operatively linked to a control element that promotes transcription of said recombinant nucleic acid in the presence of an inhibitor of cellular RNA Polymerase II;b. culturing said population of eukaryotic host cells in the presence of an effective amount of an inhibitor of cellular RNA Polymerase II, wherein said exogenous polypeptide is expressed while expression of endogenous proteins of said population of eukaryotic host cells is suppressed; andc. identifying said recombinant nucleic acid by detecting said property of interest.