Patent ID: 6815541
Filing Date: 2004-11-09
Classification: C07H,C12N

Abstract:
A method for enhancing the precipitation of nucleic acids from a biological sample, comprising the steps of:a) adding at least one coprecipitant which precipitates together with nucleic acids as a visible white or blue precipitate when separating and concentrating the nucleic acids by alcohol in a process of extracting with nucleic acids as a visible blue precipitate when separating and concentrating the nucleic acids by alcohol in a process of extracting the nucleic acids, at any of steps b-g; b) pretreating for lysing nucleic acids from biological sample; c) adding an aqueous liquid and a non-hydrophilic organic liquid having a high specific gravity and containing a thixotropic thickening agent to the pretreated biological sample; d) mixing the materials of c); e) centrifugal separation thereof; f) separating the upper phase containing nucleic acids by forming a non-flowable agglutinated phase i n a boundary interface between the upper and lower phases; g) blending the aqueous liquid containing nucleic acids by adding alcohol thereto; and h) separating and concentrating the nucleic acids together with said coprecipitant by centrifugal separation, such that a visible white or blue precipitate results.