Patent ID: 6825032
Filing Date: 2004-11-30
Classification: G01N

Abstract:
An assay platform for isolating, harvesting, detecting or quantifying a target polypeptide molecule, the platform comprising a substrate, a polymer matrix attached to the substrate, and a binding ligand attached to the polymer matrix having an affinity for the target polypeptide molecule, the binding ligand being selected from the group consisting of metal chelates, anion exchangers, cation exchangers, hydrophobic binding ligands, antibodies, streptavidin, avidin, biotin, glutathione, protein A, protein G, and protein L, wherein the polymer matrix is covalently attached directly to the substrate to provide a density of the polymer matrix on the substrate of at least 2 &mgr;g/cm2, and the density of the binding ligand attached to the polymer matrix is at least 1 nanomole/cm2.