Patent ID: 7294463
Filing Date: 2007-11-13
Classification: A01K,C07K,C12N

Abstract:
1. A method of screening for a compound that increases or decreases activity of a promoter sequence of the β-2 subunit of a neuronal nicotinic acetylcholine receptor, comprising: (a) contacting the compound with a cell line, wherein the cell line is a neuronal cell line and comprises a promoter sequence of the β-2 subunit of the neuronal nicotinic acetylcholine receptor operatively linked to a heterologous sequence encoding a polypeptide, wherein the promoter sequence comprises the nucleic acid sequence from about nucleotide −1125 to about nucleotide +38 as set forth in (b) measuring directly or indirectly the expression of the polypeptide; and (c) selecting the compound that increases or decreases expression of the polypeptide, wherein an increase or decrease in polypeptide expression correlates with an increase or decrease in activity of the promoter sequence of the β-2 subunit of the neuronal nicotinic acetylcholine receptor.