Patent ID: 6416757
Filing Date: 2002-07-09
Classification: A61K,C07K

Abstract:
An isolated antibody or fragment thereof that binds to mature human TNF-&agr;,wherein the antibody or fragment thereof is characterzed in that when the antibody or fragment thereof binds TNF-&agr; the induction of tunor fibrin deposition is enhanced; the induction of endothelial procoagulant activity is unaffected; and cytoxicity, tumor regression and tumor receptor binding are inhibited; and wherein the antibody or fragment thereof binds to TNF-&agr; such that the epitope defined by the topographic regions Pro12-Val13-Ala14-His15-Val16-Val17-Ala18-Asn19-Pro20-Gln21-Ala22, Leu36-Leu37-Ala38-Asn39-Gly40-Val41-Glu42-Leu43-Arg44-Asp45-Ala96-Ile97-Lys98-Ser99-Pro100-Cys101-Gln102-Arg103-Glu104-Thr109and Leu132-Ser133-Ala134-Glu135-Ile136-Asn137-Arg138-Pro139-Asp140-Tyr141-Leu142-Asp143-Phe144-Ala145-Glu146-Ser147-Gly148-Gln149-Val150-Tyr151-Phe152-Gly153-Ile154-Ile155-Ala156-Leu157 is prevented fom binding to mature human TNF-&agr; receptor.