Patent ID: 6548018
Filing Date: 2003-04-15
Classification: B01L,C12Q,G01N,Y10T

Abstract:
A device for use in a chemiluminescence assay for the detection of cellular contamination, the device comprising:a.) a sampling wand comprising: i. an internal reagent reservoir disposed toward a distal end of the wand; ii. an external sampling swab disposed on a surface at the distal end of the wand; and iii. a first frangible seal disposed between the sampling swab and the reagent reservoir; and b.) a reaction chamber comprising: i. an upper portion into which the sampling wand may be inserted in a fluid-tight, longitudinally slidable arrangement; ii. a lower portion; iii. a second frangible seal disposed between the upper portion and the lower portion; and iv. a reactant disc disposed within the lower portion at a distal end of the chamber; c.) means for rupturing said first and second seals; wherein, upon longitudinal movement of the sampling wand within the upper chamber to a first operative position, the first frangible seal is ruptured permitting fluid flow of a reagent solution stored within the reservoir into the upper portion of the chamber; and wherein, upon further longitudinal movement of the sampling wand to a second operative position, the second frangible seal is ruptured, permitting fluid flow of the reagent solution from the upper portion of the chamber into the lower portion of the chamber, whereupon the reagent solution contacts the reactant disc.