Patent ID: 8084196
Filing Date: 2011-12-27
Classification: A61P,G01N

Abstract:
1. A method for the in vitro screening of an anti-metastatic compound that inhibits SK3 activity comprising: (a) incubating a candidate compound to be tested with SK3; (b) assaying for the binding of the candidate compound to be tested with SK3; (c) selecting positively a candidate compound if said candidate compound binds to SK3; (d) contacting a cell expressing a functional SK3 with said candidate compound and measuring the level of SK3 activity using a cell migration assay; (e) comparing the level of SK3 activity which is measured at step (d) with the level of SK3 activity which is measured when step (d) is performed in the absence of said candidate compound, wherein a decrease of SK3 activity in the presence of the candidate compound indicates that the candidate consists of an anti-metastatic compound.