Patent ID: 7445932
Filing Date: 2008-11-04
Classification: A01H

Abstract:
1. A method for an efficient, in-vitro micropropagation of Solanum viarum producing a large number of viable plants, said method comprising the steps of: i. selecting a mother plant from healthy elite plants of ii. treating the plants growing in the field with systemic fungicides and insecticides in a conventional manner, at one week intervals, for two weeks; iii. collecting a nodal stem explants from the mother plants; iv. cleaning the collected explants; v. surface sterilizing the cleaned explants; vi. cutting the explants into small pieces of approximately 2-10 mm length or diameter; vii. inoculating the small pieces of cut explants on an initiation medium comprising an MS medium supplemented with the growth hormones like auxins selected from the group consisting of NAA, in the range of 0.01-5 mg per liter, cytokinins, selected from the group consisting of BAP 0.2-2mg per liter, and kinetin 1-10 mg per liter and/or a combination thereof; viii. incubating the explants inoculated on the initiation medium for a varied photoperiod of a day or light period ranging from 0-24 hours and a night or dark period ranging from 24-0 hours and maintaining for a period of 2-20 days, and scoring the incubated inoculants for contamination if any; ix harvesting healthy multiple shoots from the best responsive nodal explants; x. dissecting the healthy multiple shoots into 0.4 -0.8 cm size and culturing on a multiplication and shoot elongation medium to a 3-6 cm. height, wherein the multiplication and shoot elongation medium is a single medium comprising an MS medium with a combination of IAA 0.025-0.1 mg/l and BAP 0.5-3 mg/ls supplemented with growth hormones like auxin selected from the group consisting of indole acetic acid, in the range of 0.01-0.2 mg per liter, cytokinin like BAP 0.1-5.0 mg per liter and/or combinations thereof, to give cultures; xi. transferring the shoots of 3-6 cm height to a medium for rooting comprising an MS medium of 0.1-1.0 concentration strength and maintaining until the formation of a plant with well developed roots; xii. removing the plants with well developed roots from a container, washing with water to remove agar adhering to the plant, drenching with 0.02-0.2% fungicide like bavistin and planting on a soil mixture for a primary hardening for 4-8 weeks; xiii. transferring the plants outside of the green house for a secondary hardening for 2-6 weeks; and xiv. transferring the plants to a field.