Patent ID: 9204599
Filing Date: 2015-12-08
Classification: A01H,C12N,C12Q

Abstract:
1. A method for determining zygosity of a corn plant comprising an AAD-1 corn event DAS-40278-9 comprising SEQ ID NO:1, said event comprising a transgene construct comprising an AAD-1 gene, said transgene construct being flanked by a 5′ flanking corn genomic DNA and a 3′ flanking corn genomic DNA, said method comprising: obtaining a DNA sample of genomic DNA from said corn plant; producing a contacted sample by contacting said DNA sample with subjecting said contacted sample to fluorescence-based endpoint quantitative PCR conditions; quantitating said florescent event probe that hybridized to said event amplicon; quantitating said florescent reference probe that hybridized to said reference amplicon; comparing amounts of hybridized florescent event probe to hybridized florescent reference probe; and determining zygosity of said corn plant comprising event DAS-40278-9 by comparing florescence ratios of hybridized fluorescent event probe and hybridized fluorescent reference probe.