Patent ID: 8846042
Filing Date: 2014-09-30
Classification: A61K,A61P,C07K

Abstract:
1. A multi-specific Fab fusion protein comprising: a Fab fragment that binds to the N-terminus of CD3 epsilon; a fusion moiety A linked to the N-terminus of the VL of the Fab fragment and a fusion moiety B linked to the N-terminus of the VH of the Fab fragment, wherein the Fab fragment binds to an epitope within amino acids 1-27 of CD3 epsilon, wherein the fusion moiety A comprises a binding domain and wherein the fusion moiety B comprises a binding domain, and wherein: a. the Fab fragment comprises, b. the C-terminus of the fusion moiety A is covalently linked to the N-terminal end of the VL domain of the Fab fragment; and c. the C-terminus of the fusion moiety B is covalently linked to the N-terminal end of the VH domain of the Fab fragment; and d. optionally, a first linker situated between the fusion moiety A and the VL domain of the Fab fragment; and e. optionally, a second linker situated between the fusion moiety B and the VH domain of the Fab fragment.