Patent ID: 8865124
Filing Date: 2014-10-21
Classification: A61K,C07K,C12Q,G01N

Abstract:
1. A fluorescent or luminescent probe reagent for measuring oxidative stress in a cell or an organism, the reagent being a fusion protein comprising: a fluorescent or luminescent protein; a marker protein; a cleavage sequence; and a regulatory factor, wherein the marker protein makes it possible to detect the oxidative stress caused by reactive oxygen species, and comprises a regulatory factor-binding site and a ubiquitin-binding site; the regulatory factor is a protein capable of regulating degradation of the marker protein in response to the reactive oxygen species; the fluorescent or luminescent protein and the marker protein are adjacent to each other in any order so as to form a fusion molecule; the cleavage sequence is positioned between the fusion molecule and the regulatory factor and comprises a cleavable sequence that is cleaved in the cell or the organism, so that the reagent comprises a protein containing the fusion molecule and a protein containing the regulatory factor separated at the cleavage sequence; and binding or dissociation between the regulatory factor and the marker protein promotes or reduces the degradation of the marker protein, and wherein the fusion protein comprises the components positioned from N-terminus to C-terminus in one of the following orders: (1) the fluorescent or luminescent protein, the marker protein, the cleavage sequence, and the regulatory factor; (2) the marker protein, the fluorescent or luminescent protein, the cleavage sequence, and the regulatory factor; (3) the regulatory factor, the cleavage sequence, the fluorescent or luminescent protein, and the marker protein; and (4) the regulatory factor, the cleavage sequence, the marker protein, and the fluorescent or luminescent protein.