Patent ID: 8227247
Filing Date: 2012-07-24
Classification: C12N

Abstract:
1. A method of generating a population of oligodendroglial precursor cells, the method comprising the steps of: (a) differentiating isolated pluripotent stem cells into a population of early rosette cells that are Pax6+/Sox1-; (b) culturing early rosette cells in the presence of retinoic acid and an agent that activates a sonic hedgehog pathway to obtain a population of cells having a neural tube-like rosette morphology that are Pax6+/Sox1+; (c) culturing the neural tube-like rosette cells in the presence of an agent that activates a sonic hedgehog pathway and FGF-2 to obtain Olig2-expressing progenitor cells; and (d) culturing the Olig2-expressing progenitors cells in the presence of the agent that activates the sonic hedgehog pathway, but in the absence of retinoic acid and in the absence of FGF-2, to obtain PDGFRα+/SOX10+/OLIG2+/NKX2.2+ oligodendroglial precursor cells.