Patent ID: 7091023
Filing Date: 2006-08-15
Classification: C12N,C12P

Abstract:
1. An isolated esterase which is: (a) a polypeptide which has at least 95% identity with a polypeptide having an amino acid sequence as the mature peptide shown in SEQ ID NO: 2; (b) a polypeptide which has at least 95% identity with a polypeptide encoded by an esterase encoding part of the DNA sequence cloned into a plasmid present in (c) a polypeptide which is encoded by a nucleic acid sequence which hybridizes with a complementary strand of the nucleic acid sequence shown as nucleotides 629–911 and 971–2208 of SEQ ID NO: 1 in a solution of 5×SSC, 5× Denhardt's solution, 0.5% SDS and 100 micrograms/ml of denatured sonicated salmon sperm DNA, followed by hybridization in the same solution for 12 hours at approximately 60° C., followed by washing twice for 30 minutes in 2×SSC, 0.5% SDS at a temperature of at least 65° C.