Patent ID: 6054269
Filing Date: 2000-04-25
Classification: C07K,C12Q

Abstract:
A purified polynucleotide that hybridizes under stringent conditions with a nucleic acid encoding a D-alanine:D-alanine ligase of a Streptococci species selected from the group consisting of S. gordonii, S. mitis, S. mutans, S. oralis, S. salivarius, S. sanguis, S. anginosus, S. constellatus, and S. interrmedius, wherein said purified polynucleotide consists of a nucleotide sequence that:a) has from 12 to 1095 nucleotides;b) is internal to a polynucleotide of a streptococci ddl gene amplified by the following degenerate pair of primers, said polynucleotide being from about 600 to about 1100 bp in length:(SEQ ID NO:35) V1: 5'-GGX GAA GAT GGX TCX TTX CAA GGX-3' G C AG C G A - (SEQ ID NO:36) V2: 3'-TTA TGI TAI GGI CCI AAA TG-5' G A G G; wherein X represents A, T, G, or C and I represents inosine; andc) does not hybridize with a genome of a Streptococcus strain belonging to another species under said stringent hybridization conditions.