Patent ID: 7803539
Filing Date: 2010-09-28
Classification: C07H

Abstract:
1. A method of isolating and purifying nucleic acids using an immobilized polyethylene glycol (PEG)-containing hydrogel, the method comprising: immobilizing a polyethylene glycol (PEG)-containing hydrogel on a substrate; adding a mixed sample solution containing a salt and nucleic acids to the PEG-containing hydrogel immobilized substrate to bind the nucleic acids to the PEG-containing hydrogel; washing the nucleic acid-bound PEG-containing hydrogel; eluting the nucleic acids from the nucleic acid-bound PEG-containing hydrogel using an elution solvent, and recovering the eluted nucleic acids, wherein the PEG-containing hydrogel is a reaction product of PEG and a polymer represented by formula I below, wherein an incorporation ratio of the PEG is 1 to 20 mole % based on carboxyl groups formed from hydrolysis of the polymer of formula I and the PEG is bound to the carboxyl groups of the hydrolyzed polymer via an ester bond: wherein, R and n is an integer from 2 to 100,000.