Patent ID: 7888013
Filing Date: 2011-02-15
Classification: B01L,G01N

Abstract:
1. A method for analyzing a base sequence, comprising: (a) immobilizing a single-strand nucleic acid probe at a gate portion of a field-effect device and introducing a sample solution containing at least one type of nucleic acid into a sample solution well in the gate portion to conduct hybridization with the single-strand nucleic acid probe; (b) introducing a washing liquid into the sample solution well to remove unreacted nucleic acid from the sample solution well; (c) introducing enzyme Taq polymerase and one of substrates, dATP, dGTP, dCTP, and dTTP to induce elongation reaction; (d) introducing a washing liquid into the sample solution well to remove the unreacted enzyme and substrate from the sample solution well; (e) introducing a buffer solution into the sample solution well to measure changes in threshold voltage or flat-band voltage of the field-effect device; (f) returning to said step (c), introducing enzyme Taq polymerase and a different one of the substrates (dATP, dGTP, dCTP, and dTTP) into the sample solution well to induce second elongation reaction and measuring changes in threshold voltage or flat-band voltage of the field-effect device, and (g) determining a base sequence based on the changes in threshold voltage or flat-band voltage.