Patent ID: 7258982
Filing Date: 2007-08-21
Classification: C12Q

Abstract:
1. A method of genotyping HCV nucleic acids present in a biological sample comprising the steps of contacting nucleic acids of the 5′ UTR of HCV in a biological sample with multiple oligonucleotide probes under reaction conditions in which said probes specifically hybridize to target sequences in said HCV 5′ UTR nucleic acids within the region extending from the nucleotides at positions −291 to −66, if present in the biological sample, and determining whether one or more of said probes have hybridized to said nucleic acids of HCV, wherein, under said reaction conditions, said probes can distinguish among at least three genotypes of HCV selected from the group consisting of HCV type 1, HCV type 2, HCV type 3, and HCV type 4, and classify the HCV nucleic acids into any of said at least three genotypes, if any of said at least three genotypes is present in said sample.