Patent ID: 7888079
Filing Date: 2011-02-15
Classification: C12N,C12P

Abstract:
1. An enzyme, wherein the enzyme has the following properties: 1) specifically acts on Nε-acyl-L-lysine to catalyze a reaction that releases a carboxylic acid and L-lysine, and also catalyzes the reverse reaction thereof; 2) acts on epsilon-amino group of L-lysine; 3) has an optimum pH in the range of 8.0 to 9.0 for hydrolysis reaction in Tris-HCl buffer at 37° C. where Nε-acetyl-L-lysine is used as a substrate; 4) is stable in the range of pH 6.5 to 10.5 when incubated for 1 hour at 37° C. in Tris-HCl buffer; 5) has an optimum reaction temperature of about 55° C. for hydrolysis reaction in Tris-HCl buffer (pH 8.2) were Nε-acetyl-L-lysine is used as a substrate; 6) is not inactivated by a treatment at 40° C. for 60 minutes in Tris-HCl buffer (pH 8.2), and retains 75 to 85% residual activity after 60 minutes of treatment at 55° C.; 7) is inhibited by o-phenanthroline; 8) an activity thereof is increased by cobalt ions; 9) a molecular weight thereof is about 60 k as measured by SDS-polyacrylamide electrophoresis; 10) amino acid sequence of SEQ ID NO: 1 locates at the N-terminal thereof; 11) activity on Nα-acetyl-L-Lys is 2.1% or less of the activity on Nε-acetyl-L-Lys; and 12) is inhibited by L-Cys.