Patent ID: 8715743
Filing Date: 2014-05-06
Classification: A61K,A61P,C07K,C12N,G01N,Y02A

Abstract:
1. A method of producing an immortalized human B-cell expressing an antibody specific for a predetermined antigen comprising: (a) obtaining a population of human B cells, wherein the majority of the human B cells have not undergone IgM-to-IgG immunoglobulin isotype class switching; (b) contacting said population with Epstein-Barr virus (EBV) to immortalize said human B cells; (c) contacting the human B cells with a cytokine/growth factor/signaling agent cocktail to induce IgM-to-IgG immunoglobulin isotype class switching; (d) culturing cells under conditions supporting said immortalization and immunoglobulin isotype class switching; and (e) selecting an immortalized human B cell expressing an antibody specific for said predetermined antigen.