Patent ID: 6015663
Filing Date: 2000-01-18
Classification: C07K,C12N,C12Q

Abstract:
A method for differentiating any one of a first North American strain of Porcine ReproductiVe and Respiratory Syndrome Virus (PRRSV) from any one of a second North American strain of said virus wherein said first and second North American strains are selected from the pool of all known North American strains of PRRSV comprising the steps:a. reverse transcribing and amplifying the ORP 5 region of said first strain to produce an amplified cDNA of said region;b. selecting from a matrix of ORF-5 restriction enzyme patterns for said known North American strains of PRRSV at least one restriction enzyme which yields a distinctive fragment profile from said cDNA as compared to a fragment profile of a corresponding cDNA from said second strain;c. cleaving the ORF 5 cDNA of said first strain with said at least one restriction enzyme;d. comparing the restriction enzyme fragment pattern resulting from step (c) with the restriction enzyme fragment pattern produced by digesting the cDNA from said second strain with the same enzyme used in step (c); ande. differentiating said first North American strain of PRRSV from said second North American strain of PRRSV based on the comparing in step (d).