Patent ID: 6818426
Filing Date: 2004-11-16
Classification: C12N,C12P,C12Y

Abstract:
An isolated polypeptide the amino acid sequence of which comprises a sequence at least 95% percent identical to the amino acid sequence of SEQ ID NO: 2, wherein the polypeptide is a (R)-2,3-butanediol dehydrogenase that:(a) produces (R)-acetoin by acting on (2R,3R)-2,3-butanediol using nicotinamide adenine dinucleotide as a coenzyme and produces (2R,3R)-2,3-butanediol by reducing 2,3-butanedione using a reduced form of nicotinamide adenine dinucleotide as a coenzyme; (b) uses nicotinamide adenine dinucleotide as a coenzyme in an oxidation reaction; (c) uses a reduced form of nicotinamide adenine dinucleotide as a coenzyme in a reduction reaction; and (d) preferentially oxidizes a hydroxyl group of 2,3-butanediol in (R) configuration.