Patent ID: 6297221
Filing Date: 2001-10-02
Classification: A01K,A61K,A61P,C07K,C12N

Abstract:
A method for promoting angiogenesis in a mammal comprising the step of providing to a smooth muscle cell in said mammal a nucleic acid construct comprising an SM22.alpha. promoter operably linked to a nucleotide sequence encoding a polypeptide or RNA competent to induce angiogenesis, said SM22.alpha. promoter having a maximum of 441 bases and comprising:(a) bases 900-1340 of SEQ ID NO:1 or(b) a sequence hybridizing with the complement of SEQ ID NO:1 under hybridizing conditions comprising 0.02M-0.15M sodium chloride at temperatures of 50.degree. C. to 70.degree. C., such that said nucleotide sequence encoding a polypeptide or RNA, when expressed in said smooth muscle cell, is competent to promote angiogenesis in said mammal.