Patent ID: 6136558
Filing Date: 2000-10-24
Classification: C07K

Abstract:
A variant of heregulin, said variant having an amino acid sequence not found in nature and the ability to bind an ErbB receptor, wherein said variant comprises a different amino acid than in said heregulin wherein:at residue number 177 said different amino acid is A, F, W, or Y;at residue number 178 said different amino acid is A, D, E, G, L, N, P, Q, R, T V, or W;at residue number 179 said different amino acid is A, G, L, M, P, S or V;at residue number 180 said different amino acid is A, D, E, G, H, I, K, M, N, P, Q, or R;at residue number 181 said different amino acid is A, G, I, L, P, or V;at residue number 183 said different amino acid is A, G, I, L, M, S, or T;at residue number 184 said different amino acid is A, F, G, H, I, K, L, M, N, P, Q, R, S, V, or W;at residue number 185 said different amino acid is A, G, H, I, K, L, M, N, P, Q, S, T, or V;at residue number 186 said different amino acid is A, K, R, or S;at residue number 187 said different amino acid is A, E, G, I, L, M, N, P, Q, S, or T;at residue number 188 said different amino acid is A, H, K, N, or R;at residue number 195 said different amino acid is A, H, N, Q, R, or S;at residue number 197 said different amino acid is A, F, L, V, or W;at residue number 198 said different amino acid is A, H, K, R, or S;at residue number 200 said different amino acid is A, H, R, or S;at residue number 201 said different amino acid is G, H, I, L, M, P, R, S, T, or V;at residue number 205 said different amino acid is A, F, H, I, K, R, T, V, W, or Y;at residue number 206 said different amino acid is A, F, G, H, I, K, L, M, P, R, S, V, W, or Y;at residue number 207 said different amino acid is F, H, I, L, P, R, V, W, or Y;at residue number 208 said different amino acid is A, H, K, R, or S;at residue number 209 said different amino acid is G, M, P, S, T, or V;at residue number 211 said different amino acid is A, H, R, or S;at residue number 213 said different amino acid is A, C, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, W, or Y;at residue number 214 said different amino acid is A, C, D, E, F, G, H, I, K, L, M, N, P, Q, R, S, T, W, or Y;at residue number 215 said different amino acid is A, C, D, F, H, I, K, L, M, N, P, Q, R, S, T, V, W, or Y;at residue number 216 said different amino acid is A, G, L, M, P, or V;at residue number 223 said different amino acid is A, F, H, R, S, or W; orat residue number 226 said different amino acid is A, G, L, or P;wherein said residue numbers correspond to residue numbers of native human heregulin-.beta.1 (SEQ ID NO: 93) numbered from the N-terminus; andwherein said heregulin variant comprises a portion that is at least 70% identical to the portion from about residue 175 to about residue 230 of native human heregulin-.beta.1 (SEQ ID NO: 93).