Patent ID: 6218142
Filing Date: 2001-04-17
Classification: C12N

Abstract:
An isolated nucleic acid molecule encoding a polypeptide having the enzymatic activity of an RNA-directed RNA polymerase (RdRP) or encoding an enzymatically active fragment thereof, selected from the group consisting of:(a) a nucleic acid molecule coding for a polypeptide comprising the amino acid sequence of SEQ ID NO: 2;(b) a nucleic acid molecule comprising the coding region of the nucleotide sequence of SEQ ID NO: 1;(c) a nucleic acid molecule that specifically hybridizes to a complementary strand of the nucleic acid molecule as defined in (a) or (b) in 0.25 M NaHPO.sub.4 pH7.2; 0.25 M NaCl, 7% SDS, 1 mM EDTA and 5-20% (w/v) polyethylene glycol (M.sub.r 6-7.5.times.10.sup.3) at 42.degree. C. for 4-24 hours; and(d) a nucleic acid molecule, the nucleotide sequence of which is degenerate as a result of the genetic code to a nucleotide sequence of the nucleic acid molecule as defined in (c).