Patent ID: 6708119
Filing Date: 2004-03-16
Classification: C12N

Abstract:
A method for analyzing base sequence date and removing, based on said data, a vector base sequence from a recombinant DNA base sequence, comprising:storing data identifying each of restriction enzymes and data of base sequences at a plurality of restriction enzyme sites of a plurality of vectors correspondingly, in a database; searching base sequence data of a recombinant DNA obtained by splicing an object DNA fragment into a vector; obtaining the base sequence data at a front restriction enzyme site and the base sequence data at a back restriction enzyme site, as specified by corresponding to a restriction enzyme used for cleaving the vector and a restriction enzyme used for obtaining the object DNA fragment, from the database; generating a first forward retrieval key using the obtained base sequence data of the front restriction enzyme site and a first backward retrieval key using the obtained base sequence data of the back restriction enzyme site; retrieving base sequence date of the recombinant DNA obtained by a search using the first forward and first backward retrieval keys, and specifying a junction between the vector and the object DNA fragment; removing the vector base sequence at the specified junction, wherein sequence data of the first forward retrieval key and of the first backward retrieval key are generated by base sequence data of the vector entered in a vector database, data of a multiple cloning site in the vector, and data of a restriction enzyme site in the multiple cloning site, wherein data of a forward base sequence from a cleaving point in the restriction enzyme site in the multiple cloning site of the vector are acquired from the database, and a second forward retrieval key is generated using the acquired forward base sequence data of the cleaving point of the restriction enzyme site of the vector, performing first homology retrieval on condition that a first similarity value between retrieval base sequence data of the recombinant DNA and the first forward and first backward retrieval keys is equal to or larger than a predetermined value, obtaining a candidate for a base sequence at the junction between the vector and the object DNA fragment according to a result of the first homology retrieval, and performing a second homology retrieval on condition that a second similarity value between base sequence data of a plurality of first candidates for the junction, and base sequence data of the second forward retrieval key, is equal to or larger than a predetermined value.