Patent ID: 8846869
Filing Date: 2014-09-30
Classification: C07K,G01N

Abstract:
1. A mutant protein capable of binding specifically to troponin I derived from human myocardium, the mutant protein comprising: a first mutant scFv antibody fragment; a second mutant scFv antibody fragment; and a linker, wherein the first mutant scFv antibody fragment comprises a first light chain variable region consisting of an amino acid sequence represented by SEQ ID NO: 76 and a first heavy chain variable region consisting of an amino acid sequence represented by SEQ ID NO: 77; the second mutant scFv antibody fragment comprises a second light chain variable region consisting of an amino acid sequence represented by SEQ ID NO: 78 and a second heavy chain variable region consisting of an amino acid sequence represented by SEQ ID NO: 79; the linker comprises cysteine molecules at the N-terminus and C-terminus thereof; the linker is provided between the C-terminus of the first heavy chain variable region and the C-terminus of the second heavy chain variable region; the linker is bound to the C-terminus of the first heavy chain variable region through a disulfide bond; and the linker is bound to the C-terminus of the second heavy chain variable region through a disulfide bond.