Patent ID: 7943323
Filing Date: 2011-05-17
Classification: C07K,G01N,Y10S

Abstract:
1. A method for producing an antibody for specifically detecting whole PTH 1-84 while avoiding detecting an interfering non-(1-84) parathyroid hormone fragment in a biological sample, which method comprises: a) administering a first peptide or protein immunogen to a host animal to induce antibody production against said first peptide or protein immunogen in said host animal, said first peptide or protein immunogen being human, rat, bovine, or porcine PTH b) monitoring antibody titer produced by said administration of said first peptide or protein immunogen to said host animal; c) extracting antiserum produced in said host animal by said administration of said first peptide or protein immunogen; and d) purifying said antiserum and selecting an antibody from said antiserum extracted in step c) by affinity chromatography utilizing a second peptide or protein immunogen, which is human PTH