Patent ID: 6174726
Filing Date: 2001-01-16
Classification: A61P,C12N

Abstract:
Method for the preparation of a conditionally immortalized immortalization-helper cell (fuseme) including at least the following steps:(a) introducing immortalizing genes into mammalian cells in a way that at least the expression and/or function of at least one of said genes may be controlled in order to obtain conditionally immortalized mammalian cells;(b) introducing at least two selectable markers, one for positive and one for negative selection, into the conditionally immortalized mammalian cells to enable selection among fuseme cells, cells to be immortalized using the fuseme cells and cells immortalized by the fuseme cells;(c) selecting for such cells (fusemes) carrying the two selectable markers introduced in step (b), the immortalizing gene introduced in step (a), and being conditionally immortalized.