Patent ID: 7700822
Filing Date: 2010-04-20
Classification: C12N

Abstract:
1. A method of assessing the effect of a drug, toxin or chemical, the method comprising exposing a conditional liver specific cytochrome P450 reductase (CPR) null homozygous transgenic mouse to a drug, toxin or chemical and comparing results of a selected parameter to results obtained from exposing a wild-type mouse to the same drug, toxin or chemical, wherein the selected parameter is selected from the group consisting of: (i) elimination rate of the drug, toxin or chemical or its metabolite(s); (ii) circulatory levels of the drug, toxin or chemical or its metabolite(s); (iii) disposition of the drug, toxin or chemical or its metabolite(s); (iv) drug, toxin or chemical bioavailability; (v) rate of metabolism of the drug, toxin or chemical; (vi) rate of clearance; (vii) extrahepatic contribution to metabolic rate and clearance of the drug, toxin or chemical or its metabolite(s); (viii) efficacy of the drug, toxin or chemical; (ix) toxicity of the drug, toxin or chemical; and (x) role of cytochrome P450 system in endogenous metabolism, and the comparison of the results provides information regarding the drug, toxin or chemical as it relates to the selected parameter, wherein the conditional liver specific cytochrome P450 reductase null homozygous transgenic mouse is produced by crossing a CPR