Patent ID: 7906333
Filing Date: 2011-03-15
Classification: C12N

Abstract:
1. A surface modification method of polysaccharide, comprising: (a) immersing a solidified, crosslinked polysaccharide material in a 0.6 N HCl aqueous solution to protonize the solidified, crosslinked polysaccharide material; (b) after step (a), immersing the solidified, crosslinked polysaccharide material in an acetic acid solution containing a protein to mechanically embed the protein into the surface of the solidified, crosslinked polysaccharide material which is protonized; and (c) after step (b), immersing the solidified, crosslinked polysaccharide material in an alkaline solution containing bivalent metal ions to limit the embedded protein to the surface of the solidified, crosslinked polysaccharide material.