Patent ID: 6428667
Filing Date: 2002-08-06
Classification: C07D,C09B,C12Q,G01N,Y10T

Abstract:
In a method for performing a separation of a composition comprising employing an electrical field for directing movement of components in said composition in a differential manner, the improvement which comprises:having present during said separation in said electrical field a moiety comprising (a) a probe having a double stranded DNA of at least about 20 base pairs and (b) non-covalently bound intercalated in said double stranded DNA at least one fluorescent molecule characterized by having at least two positive charges, having at least one fluorescent monomeric unit and having a binding affinity to double stranded DNA of at least about 5Ã—106 Mâˆ’1 and up to about 1 fluorescent unit per 4 nucleotides in a nucleic acid strand, wherein said fluorescent unit is characterized by having at least one aromatic ring per monomeric unit.