Patent ID: 9163230
Filing Date: 2015-10-20
Classification: C07H,C12N

Abstract:
1. A method for isolating nucleic acid, comprising: a) providing a chromatography column comprising a first solid layer and a second solid layer which are serially coupled so that solutions flow through the first layer and then the second b) flowing a first aqueous solution containing nucleic acid through the first layer to which the nucleic acid becomes bound, c) flowing a second aqueous solution through the first layer and eluting the bound nucleic acid, and then flowing the eluted solution through the second layer to which the eluted nucleic acid becomes bound, wherein the second solution comprises chaotropic salt selected from the group consisting of guanidinium thiocyanate, guanidinium hydrochloride, sodium iodide, and sodium perchlorate, and d) flowing a third aqueous solution through the second layer and eluting the bound nucleic acid.