Patent ID: 7951536
Filing Date: 2011-05-31
Classification: C12Q

Abstract:
1. A method for determining the base sequence of a DNA, comprising: (a) the step of ligating a DNA to be analyzed, wherein said DNA has a one base-protruding end and is immobilized on a support, and a probe by a DNA ligase, wherein said probe has a one base-protruding end and an identification-label according to the species of the base at the protruding end, and said probe contains a recognition sequence of a restriction enzyme whose recognition sequence is apart from a cleavage site; (b) the step of identifying the species of the identification label of said probe that has been ligated to said DNA to be analyzed by single molecule observation; and (c) the step of cleaving a ligated DNA of said DNA to be analyzed and said probe by said restriction enzyme whose recognition sequence is apart from the cleavage site.