Patent ID: 6143508
Filing Date: 2000-11-07
Classification: A61K,C12M,C12N,Y10S

Abstract:
A method for preparing a separated cell population from a mixture of cells, comprising:(a) contacting said mixture of cells with a device comprising at least one, optically clear polystyrene film or sheet, internal to the device, having support having a plurality receptors covalently bound to both flat surfaces thereof, which device has the following properties:(i) the covalent bonding is produced by derivatizing the benzene rings of polystyrene which is less than 0.5% crosslinked;(ii) the receptors are at a density of between about 200 ng receptor per cm.sup.2 surface area and about 250 ng receptor per cm.sup.2 surface; and(iii) the receptors bind specifically to at least one cell-surface ligand,said contacting being for sufficient time for cells bearing said ligand to bind specifically to said receptors on said flat surfaces, resulting in specifically bound cells and unbound cells;(b) removing said unbound cells from said device without significantly disturbing the binding of said specifically bound cells to said surface such that said bound cells remain bound to said device; and(c) releasing said bound cells from said device such that released cells are free of said receptors,thereby preparing said separated cell population.