Patent ID: 8378088
Filing Date: 2013-02-19
Classification: A61P,C12N,C12Q

Abstract:
1. An isolated synthetic duplex microRNA mimetic comprising: (i) a guide strand nucleic acid molecule consisting of a nucleotide sequence of 18 to 25 nucleotides, said guide strand nucleotide sequence comprising a seed region nucleotide sequence and a non-seed region nucleotide sequence, said seed region consisting of nucleotide positions 1 to 12 and said non-seed region consisting of nucleotide positions 13 to the 3′ end of said guide strand, wherein position 1 of said guide strand represents the 5′ end of said guide strand, wherein said seed region comprises a consecutive nucleotide sequence of at least 6 nucleotides that is identical to a seed region sequence of a naturally occurring microRNA; and (ii) a passenger strand nucleic acid molecule consisting of a nucleotide sequence of 18 to 25 nucleotides, said passenger strand comprising a nucleotide sequence that is essentially complementary to the guide strand, wherein said passenger strand nucleic acid molecule has one nucleotide sequence difference compared with the true reverse complement of the seed region of the guide strand, wherein the one nucleotide difference is located within nucleotide position 13 to the 3′ end of the passenger strand.