Patent ID: 7776588
Filing Date: 2010-08-17
Classification: A01K,A61K,C12N,C12Q,G01N

Abstract:
1. A method of screening for whether a compound has a biological effect in a canine cell having a truncated P-gp protein, comprising: contacting a first canine cell with the compound, wherein the first canine cell has a truncation mutation in a mdr1 gene at residues 294-297 of SEQ ID NO: 1, which mutation results in the canine cell having a truncated P-gp protein, to produce a contacted canine cell; and comparing a characteristic of the contacted canine cell with a) the same characteristic of the first canine cell prior to contact with the compound, b) the same characteristic of a similar canine cell not contacted with the compound, or c) the same characteristic of a second canine cell contacted with the compound, wherein the second canine cell does not have a truncation mutation in its mdr1 gene; wherein a difference in the characteristic indicates the compound has a biological effect in the canine cell having the truncated P-gp protein.