Patent ID: 7754467
Filing Date: 2010-07-13
Classification: A61K,A61P,C07K,C12N

Abstract:
1. An isolated Lactobacillus jensenii bacterium comprising an expression cassette, the expression cassette comprising a promoter operably linked to polynucleotide encoding a signal sequence and a biologically-active polypeptide, wherein the biologically active polypeptide is expressed, is anchored to the cell wall of the Lactobaeillus bacterium or is released from the Lactobacillus bacterium, and is linked to a heterologous carboxyl terminal cell wall targeting region and wherein the cell wall targeting region comprises sequence selected from group consisting of SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO: 7 in which LPQTG (SEQ ID NO:13) is replaced with LPQSG, LPQAG or LPQTA (SEQ ID NO:11, 12, 14, respectively) and SEQ ID NO: 8 in which LPQTG (SEQ ID NO:13) is replaced with LPQSG, LPQAG, or LPQTA (SEQ ID NO:11, 12 or 14, respectively), and wherein the biologically active protein binds to a pathogen when the biologically active protein is contacted with the pathogen.