Patent ID: 8962338
Filing Date: 2015-02-24
Classification: C07C,G01N

Abstract:
1. A method of measuring calcium ion concentration in a sample, the method comprising the steps of a) mixing a sample with a solution thereby forming a mixture, the solution having a pH in a range of from 9.0 to 10.5 and comprising a compound having a chemical structure represented by Formula I: wherein R b) incubating the mixture for a period of time, whereby calcium ions within the sample bind to the compound; c) measuring a baseline absorbance value of the mixture; d) adding a releasing agent to the mixture; e) incubating the mixture of the sample, the solution, and the releasing agent for a period of time, whereby calcium ions release from the compound; f) measuring a second absorbance value of the mixture of the sample, the solution, and the releasing agent; g) calculating a difference in the baseline absorbance value and the second absorbance value; and h) determining a concentration of calcium ions within the sample based on said step of calculating.