Patent ID: 7015013
Filing Date: 2006-03-21
Classification: G01N

Abstract:
1. A method for localized staining of an intact tissue surface comprising the steps of: positioning a staging device on said intact tissue surface so that the staging device defines a desired treatment area; deprotonating ε-amino groups of lysine residues on exposed proteins of a desired treatment area of said intact tissue surface, without an resulting softening of said intact tissue surface; applying a chemical dye to said desired treatment area, wherein said chemical dye reacts with said tissue resulting in covalent bonding of said chemical dye to the deprotonated ε-amino groups of lysine residues on said exposed proteins; and rinsing the desired treatment area to remove unreacted chemical dye, said reacted chemical dye remaining in the desired treatment area to create a dark stain on said intact tissue surface.