Patent ID: 6372720
Filing Date: 2002-04-16
Classification: A61K,C07K,C12N

Abstract:
A liposome complex comprising:a fusogenic peptide, wherein the fusogenic peptide is selected from the group consisting of a peptide comprising the amino acid sequence of SEQ ID NO: 7 and variants of an N-terminal pulmonary surfactant B (SP-B) peptide, wherein said N-terminal SP-B peptide consists essentially of the first 25 amino acids of the N-terminus of an SP-B protein, wherein each of said variants comprises one or more changes to the native sequence of said N-terminus selected from the group consisting of: 1) substitution of one or more lysine and/or one or more arginine of the hydrophilic stripe of the amphipathic alpha helix with histidine; 2) substitution of one or more leucine and/or one or more isoleucine from the hydrophobic stripe of the amphipathic alpha helix with norleucine; 3) lengthening the membrane anchoring sequence in the first 6 positions of the N-terminal by inserting additional membrane anchoring amino acids; and 4) replacing the C-terminus carboxylic acid group with an amide group; a non-cationic lipid; and a substance to be delivered into a cell.