Patent ID: 6670189
Filing Date: 2003-12-30
Classification: A01N,C12N

Abstract:
A method of identifying transformed plant calls comprising the steps of:a) introducing into a plant cell at least one copy of an expression cassette comprising a coding region that codes for a fumonisin esterase enzyme operably linked to a promoter, wherein said fumonisin esterase enzyme is selected from the group consisting of; i) a nucleotide sequence set forth in SEQ ID NO: 11; ii) a nucleotide sequence encoding an amino acid sequence as set forth in SEQ ID NO: 12; iii) a nucleotide sequence set forth in SEQ ID NO: 15; iv) a nuelcotide sequence encoding an amino acid sequence as set forth in SEQ ID NO: 16; v) a nucleotide sequence with fumonisin esterase activity that has at least 95% sequence identity to the full length of a sequence set forth in (i), (ii), (iii), or (iv); and; b) introducing a fumonisin or a structurally related analog of fumonisin into the culture medium; and c) identitng transformed cells as the surviving cells in the culture.