Patent ID: 6261773
Filing Date: 2001-07-17
Classification: C12Q

Abstract:
A reagent for nucleic acid amplification comprising a forward primer having a DNA sequence homologous to a sequence of a target RNA; a reverse primer having a DNA sequence complementary to a sequence of the target RNA and having a promoter for RNA polymerase attached to its 5' end; ribonucleotides; deoxyribonucleotides; a reverse transcriptase or RNA directed DNA polymerase; a RNase H; a DNA polymerase or reverse transcriptase having DNA-directed DNA polymerase activity; a RNA polymerase; and at least one member selected from the group consisting of ethylenediamine tetraacetic acid, nitrirotriacetic acid, uramil diacetic acid, trans-1,2-cyclohexanediaminetetraacetic acid, diethylenetriaminepentaacetic acid, ethyleneglycol bis(2-aminoethyl)ether diaminetetraacetic acid, triethylene tetraminehexaacetic acid and a salt thereof, wherein said at least one member is present at a final concentration of from 0.5 to 60 mM in the reaction mixture for nucleic acid amplification.