Patent ID: 6207365
Filing Date: 2001-03-27
Classification: C12Q

Abstract:
An enzyme substrate represented by the following formula:(BLOCK-O)-X.sub.cuwherein BLOCK is a blocking group selected from the group consisting of:a monovalent blocking group derivable by removal of one hydroxyl from a phosphoric acid group, a sulfuric acid group, or a salt thereof; and a monovalent blocking group derivable by removal of a hydroxyl from an aliphatic carboxyl, an aromatic carboxyl, an amino acid carboxyl, or a peptide carboxyl, wherein said BLOCK is cleaved from said substrate by the action of an enzyme to yield a HO--X.sub.cu product, and further, said HO--X.sub.cu product intramolecularly forms a lactone ring to provide a coumarin derivative;wherein X.sub.cu has a structure represented by the following formula and is covalently bound to oxygen O at C.sup.1 carbon, ##STR36##wherein L represents H, NH, alkyl having 1 to 4 carbons, tetralkylammonium having 1 to 4 carbons, or an alkaline metal or an alkaline earth metal; and further,wherein said coumarin derivative has a structure represented by the following formula: ##STR37##