Patent ID: 6060593
Filing Date: 2000-05-09
Classification: C07K,C12N

Abstract:
A nucleic acid encoding a chimeric polypeptide comprising an epitope or epitores of GAD65 protein, said epitope selected from the group consisting of an epitope present in amino acids 270-435, an epitope present in amino acids 451-570, an epitope present in amino acids 221-585, an epitope present in amino acids 270-442, an epitope present in amino acids 443-513, an epitope present in amino acids 528-585, an epitope present in amino acids 451-585, and an epitope present in amino acids 221-442, and a structural region comprising a polypeptide from a GAD67 protein, wherein the chimeric polypeptide is a more specific diagnostic for insulin dependent diabetes mellitus than intact GAD65 and produces fewer false positives than intact GAD65.