Patent ID: 8962253
Filing Date: 2015-02-24
Classification: C12Q

Abstract:
1. A method of detecting the presence of one or more target RNAs in a sample, said method comprising: a) ligating an adapter or linker oligonucleotide to the 3′ end of said one or more target RNAs to produce one or more extended target polynucleotides; b) circularizing said one or more extended target polynucleotides by ligating the 5′-end of the one or more extended target polynucleotides to its 3′- end; c) synthesizing by rolling circle amplification complementary multimeric nucleic acids (MNAs) comprising multiple repeats of sequences that are complementary to said one or more circularized extended polynucleotides; and d) detecting said MNAs, thereby detecting the presence of said one or more target RNAs in said sample.