Patent ID: 7598082
Filing Date: 2009-10-06
Classification: C12N

Abstract:
1. A method of preparing a reprogrammed diploid mammalian cell comprising providing a diploid mammalian donor nucleus derived from a somatic cell, and a mammalian recipient cell, wherein the diploid mammalian donor nucleus and the mammalian recipient cell are of the same species; introducing the mammalian donor nucleus into the mammalian recipient cell to produce an aneuploid cell; maintaining the aneuploid cell in a suitable environment for a period sufficient to allow the donor nucleus to be reprogrammed; subjecting the aneuploid cell to an activation step; and subsequent to maintaining the aneuploid cell in the suitable environment, treating said reprogrammed aneuploid cell so as to generate a reprogrammed diploid mammalian cell from said reprogrammed aneuploid cell by removal or destruction of the mammalian recipient cell nucleus, pronucleus, metaphase plate, chromatin, chromosomes or nuclear DNA from said reprogrammed aneuploid cell.