Patent ID: 8741600
Filing Date: 2014-06-03
Classification: B01D,C07K

Abstract:
1. A method of separating an immunoglobulin monomer comprising filtering an immunoglobulin solution containing at least an immunoglobulin monomer and an immunoglobulin aggregate and having an immunoglobulin concentration of 1 to 150 g/L with cross-flow filtration using an ultrafiltration membrane having a molecular weight cut-off of 100,000 or more and less than 500,000, forming a cake layer on the surface of the ultrafiltration membrane, maintaining the immunoglobulin concentration in an immunoglobulin stock solution during filtration within the range of 50 to 200 when the immunoglobulin concentration before filtration is referred to as 100, and passing the immunoglobulin monomers through the cake layer to obtain an immunoglobulin permeation solution.