Patent ID: 7479276
Filing Date: 2009-01-20
Classification: A61K,A61P,C12N

Abstract:
1. A nucleic acid-cationic immunoliposome complex comprising i) a cationic liposome, ii) an scFv antibody fragment, and iii) a nucleic acid wherein said nucleic acid-cationic immunoliposome complex is prepared by a method comprising: a) preparing said antibody fragment; b) directly conjugating said antibody fragment to said cationic liposome to form a cationic immunoliposome, wherein said conjugation occurs via a sulfur atom which was part of a sulfhydryl group at the carboxy terminus on said antibody fragment prior to said conjugation; and c) mixing said cationic immunoliposome with said nucleic acid to form said nucleic acid-cationic immunoliposome complex; wherein said antibody fragment and said cationic liposome are present at a protein:lipid ratio (w:w) in the range of 1:10 to 1:40 and wherein said nucleic acid and said cationic liposome are present at a nucleic acid:lipid (μg:nmol) ratio in the range of 1:6 to 1:20.