Patent ID: 6358691
Filing Date: 2002-03-19
Classification: C12Q

Abstract:
A method, comprising:a) providing: i) target nucleic acid comprising first and second non-contiguous single-stranded regions separated by an intervening region comprising a double-stranded portion; ii) a bridging oligonucleotide comprising a first portion complementary to said first non-contiguous single-stranded region of said target nucleic acid and a second portion complementary to said second non-contiguous single-stranded region of said target nucleic acid; and iii) a reactant selected from the group consisting of polymerases and ligases; and b) mixing said target nucleic acid, said bridging oligonucleotide and said reactant under conditions such that said bridging oligonucleotide hybridizes to said target nucleic acid and wherein said bridging oligonucleotide is modified to produce a modified oligonucleotide.