Patent ID: 7781394
Filing Date: 2010-08-24
Classification: C12Q,G01N

Abstract:
1. A method for identifying a substance with antitumor activity, comprising contacting at least one sample comprising an rcl deoxynucleoside 5′-monophosphate N-glycosidase or a cell expressing rcl deoxynucleoside 5′-monophosphate N-glycosidase with the substance; measuring the level of enzymatic activity of deoxynucleoside 5′-monophosphate N-glycosidase in the sample; comparing the deoxynucleoside 5′-monophosphate N-glycosidase in the sample with at least one control sample comprising an rcl deoxynucleoside 5′-monophosphate N-glycosidase or a cell expressing rcl deoxynucleoside 5′-monophosphate N-glycosidase without the substance; selecting the substance which reduces in at least one sample deoxynucleoside 5′-monophosphate N-glycosidase activity compared to a control sample which had not be contacted with the substance; administering the selected substance to an animal comprising a tumor; evaluating at least one of indicia selected from the group consisting of size of the tumor, growth of the tumor, rate of growth of the tumor, and regression of the tumor; and comparing the at least one indicia to an animal also comprising a tumor but to which the selected substance was not administered, wherein a decrease in size and/or decrease in the rate of tumor growth is indicative that the substance has antitumor activity.