Patent ID: 8278035
Filing Date: 2012-10-02
Classification: C12N,C12Q

Abstract:
1. A method for isolating a microRNA of interest ( 42 ) from a sample comprising the microRNA of interest ( 42 ) according to FIG. 1 through FIG. 6 ; the method comprising: a) providing a sample comprising the microRNA of interest ( b) providing a capture probe ( where the microRNA binding segment ( where the 5′ end of the first adapter segment ( where the 3′ end of the second adapter segment ( c) providing a first linker ( d) providing a second linker ( e) combining the sample, the capture probe ( f) allowing the first linker ( g) ligating the 3′ end of the first linker ( h) dehybridizing the capture probe ( i) purifying the ligated first linker-microRNA of interest-second linker (