Patent ID: 6174708
Filing Date: 2001-01-16
Classification: C07K,C12N,C40B

Abstract:
A method for the production of multicombinatorial libraries wherein,(a) a first repertoire of genes coding for a population of one of two types of polypeptides, which can covalently or otherwise combine, and 2) at least one gene coding for the other type of polypeptide or a second repertoire of genes coding for a population of said other type are provided;(b) the genes of said first repertoire are introduced into a first starting vector to form a population of final vectors carrying different genes of said first repertoire;(c) said gene of said other type or genes of said second repertoire are introduced into a second starting vector;(d) and said first and second starting vectors are recombined under conditions under which one of the vectors will contain after recombination one gene for one of the two types and one gene for the other type and the two genes are expressed in the form of associated polypeptides which can be displayed on the surface of said vector and remain there, being combined together multimerically or so as to simulate a multimer; thereby producing a multicombinational library;said first and second vectors including means for exchanging one portion of each vector by irreversible recombination(s) to generate recombinant final vectors after the recombination(s), wherein one of said vectors contains a gene of one of the two types and a gene of the other type, wherein the starting vectors respectively contain two attP lambda phage sites and two attB E. coli sites, arranged so as to allow two recombination events under the influence of an associated recombinase or integrase to form two sequences of stable attachment in each of the final vectors resulting from recombination, the two recombination sites in each of the starting vectors being in opposite orientation.