Patent ID: 6720192
Filing Date: 2004-04-13
Classification: G01N,Y10S

Abstract:
A competition assay method for assaying an analyte in a sample, said analyte being a compound capable of binding to a receptor, and being neither an antibody nor an enzyme, said method comprising the elements of:a) contacting the sample with a receptor preparation comprising solubilized receptor reconstituted into liposomes, the receptor preparation comprising a receptor specific to the analyte and comprising a detectable functionality wherein the sample contacting is effected under conditions resulting in binding of the receptor to the analyte if the analyte is present in the sample; b) concomitantly with, or subsequently to, performing element a) contacting the sample with an immobilized ligand capable of binding with the receptor under conditions resulting in binding of the receptor to the ligand; c) subsequently to performing elements a) and b), separating the resulting immobilized ligand-receptor phase from the receptor phase; and d) assaying the liposome detectable functionality to determine the presence or quantity of the analyte in the sample.