Patent ID: 8153358
Filing Date: 2012-04-10
Classification: C12N

Abstract:
1. A method, comprising: (a) providing a library of polynucleotide fragments in which one or more polynucleotide fragments comprise a target gene encoding a protein with endonuclease activity; (b) encapsulating the library of fragments in a plurality of aqueous droplets in an emulsion, wherein each of the aqueous droplets in the plurality of droplets contains: (c) allowing the target gene from the library to be transcribed and translated to form the protein such that the protein permits the polynucleotide fragment containing the target gene to become covalently linked to a polynucleotide adaptor at one end by a ligase by means of a sticky end on the polynucleotide fragment resulting from the endonuclease activity of the protein, and a complementary sticky end on the polynucleotide adaptor; and (d) amplifying the target gene using an adaptor-specific primer and a fragment-specific primer.