Patent ID: 7611838
Filing Date: 2009-11-03
Classification: C12Q,G01N

Abstract:
1. A method of identifying a set of biologically-active DNA-binding sites for a protein of interest in the genome of a cell, the method comprising (i) identifying a set of regions of genomic DNA to which the protein of interest is bound in the cell; (ii) identifying candidate DNA-binding sites in the identified regions of genomic DNA, wherein a candidate DNA-binding site comprises a sequence corresponding to a DNA-sequence motif for the protein of interest, and wherein said DNA sequence motif is enriched in said identified regions by a statistically significant amount relative to a set of genomic regions which are bound by a mutant form of the protein of interest in the cell; (iii) determining if the candidate DNA-binding sites are conserved in an equivalent genomic region in one or more species different from the species from which the cell is obtained, wherein a candidate DNA-binding site that is conserved in at least one of the different species is a biologically-active DNA-binding site.