Patent ID: 6287822
Filing Date: 2001-09-11
Classification: C12Q

Abstract:
A method for mutation detection of a double stranded DNA fragment by Denaturing Matched Ion Polynucleotide Chromatography, the method comprising the steps of:(a) analyzing the wild type sequence of the double stranded DNA fragment to mark off the wild type sequence of the double stranded DNA fragment into sample sequences of nucleotides having a range of melting point of less than 15 degrees C., each sample sequence having a first end and a second end opposite thereto, wherein a melting point is the temperature at which a base pair is at 50/50 equilibrium between the helical and melted states, wherein the location of said mutation is between said first and said second ends, and wherein a sample sequence has a length of 150 to 600 base pairs;(b) amplifying a section of said double stranded DNA fragment corresponding to one of said sample sequences by PCR using a set of primers which flank the ends of said section; and(c) analyzing the amplification product of step (b) by Denaturing Matched Ion Polynucleotide Chromatography.