Patent ID: 6218182
Filing Date: 2001-04-17
Classification: A61F,A61M,C12M,C12N

Abstract:
A method of growing and using a three-dimensional tissue, comprising:providing a bioreactor comprising a housing having a mesh portion disposed therein to define separate flow spaces on opposite sides of the mesh portion;seeding cells on the mesh portion;flowing a first media having a first concentration of solutes in contact with the cells on one side of the mesh;flowing a second media flow having a second concentration of solutes in contact with the cells on the opposite side of the mesh portion, wherein the first concentration of solutes is different than the second concentration of solutes causing movement of the solutes through said cells by diffusion; andgrowing a three-dimensional tissue having multifunctional cells, wherein the development of the multifunctional cells is polarized with respect to the media flows due to exposure to diffused solutes.