Patent ID: 7695936
Filing Date: 2010-04-13
Classification: A61K,A61P,C07K

Abstract:
1. A method of preparing a heteromultimer comprising a first polypeptide and a second polypeptide each comprising a C H 3 antibody constant domain, wherein said polypeptides meet at an interface, comprising the steps of: (a) altering the nucleic acid encoding at least one amino acid residue of the first polypeptide selected from the group consisting of amino acid residues 366, 405, and 407, according to the amino acid numbering as shown in (b) altering the nucleic acid encoding at least one amino acid residue of the second polypeptide selected from the group consisting of amino acid residues 407 if amino acid residue 366 is altered in the first polypeptide, amino acid residue 394 if amino acid residue 405 is altered in the first polypeptide, and amino acid residue 366 if amino acid residue 407 is altered in the first polypeptide, according to the amino acid numbering as shown in (c) expressing said nucleic acid in a host cell to obtain the heteromultimer; (d) recovering the heteromultimer from the host cell culture.