Patent ID: 6274872
Filing Date: 2001-08-14
Classification: G01N

Abstract:
Device for carrying out quantitative fluorescence-marked affinity tests by means of evanescent field excitation, having at least one light source (7, 7') which emits almost monochromatic light and directs light beams, having a wavelength which causes fluorescence of a marking substance bound to a chemical or biochemical partner of a general receptor/ligand system, at an angle .alpha. to be defined by a predeterminable penetration depth d for the evanescent field onto an interface (20) of an optically transparent baseplate (1) made of a material whose refractive index n.sub.1 is greater than the refractive index n.sub.2 of the material above the interface (20), characterized in that the light is directed through the baseplate (1) onto the interface (20) of a reception region (2) which is in the form of a cuvette and has a thickness of between 0.001 and 0.5 mm, a sample container (10) for receiving the sample is arranged in such a way that a link is formed via a first possible connection (9), which is at least partially an opening between the reception region (2) in the form of a cuvette and the sample container (10) and a second possible connection (11) is linked to the reception region (2) in the form of a cuvette, the reception region (2) being covered on the opposite side from the baseplate (1) with a cover plate (3), and a detector (5) for picking up the fluorescent light being arranged on the same side of the baseplate (1) as the light source (7, 7').