Patent ID: 6190658
Filing Date: 2001-02-20
Classification: A61K,C07K,C12N,C12Y

Abstract:
An isolated mutant manganese superoxide dismutase protein comprising an enzymatically active portion of manganese superoxide dismutase (MnSOD) and a region which binds to polyanionic polysaccharides or proteoglycans on endothelial cell surfaces, wherein said protein is encoded by a nuclcleic acid molecule that hybridizes under conditions that achieve hyridization permitting 30% or less mismatch of nucleotides, to a nucleic acid molecule selected from the group consisting of a nucleic acid molecule comprising both nucleic acid sequences SEQ ID NO:1 and SEQ ID NO:4, a nucleic acid molecule comprising a nucleic acid sequences SEQ ID NO:6, and a nucleic acid molecule comprising a nucleic acid sequences SEQ ID NO:8, wherein said conditions comprise hybridizing in a soluition comprising 6.times.SSC or 6.times.SSPE at a temperature 20-25.degree. C. below T.sub.m, and washing at 12-20.degree. C. below said T.sub.m.