Patent ID: 6689595
Filing Date: 2004-02-10
Classification: C07K,C12N,Y10S

Abstract:
A method for crystallizing Staphylococcus aureus (S. aureus) thymidylate kinase comprising:preparing purified S. aureus thymidylate kinase at a concentration of about 1 mg/ml to about 50 mg/ml; and crystallizing S. aureus thymidylate kinase from a solution comprising the purified S. aureus thymidylate kinase, about 5 wt. % to about 50 wt. % poly(ethylene glycol) (PEG), about 0.05 M to about 0.5 M MgCl2, and about 0 wt. % to about 20 wt. % dimethyl sulfoxide. (DMSO), wherein the solution is buffered to a pH of about 6 to about 7.