Patent ID: 8993714
Filing Date: 2015-03-31
Classification: C07K,G01N

Abstract:
1. A biotinylated streptavidin macromolecular adaptor (SAMA) protein, comprising: a SAMA protein having a first end and a second end opposite of the first end, a dyad axis spanning from the first end to the second end, a pair of designated surface amino acid residues at the first end, and a pair of ligand binding sites at the second end; and a biotin-type group covalently bonded to each designated surface amino acid residue and positioned to bind to a biotin binding site of a streptavidin tetramer, and/or a biotin-ligand crosslinking reagent having a ligand moiety bound to each ligand binding site and having a biotin-type moiety positioned to bind to a biotin binding site of a streptavidin tetramer, wherein the pair of designated surface amino acid residues is symmetric about the dyad axis at the first end, and the pair of ligand binding sites is symmetric about the dyad axis at the second end.