Patent ID: 6670117
Filing Date: 2003-12-30
Classification: G01N

Abstract:
A method for detecting anti-rubella IgM antibodies, comprising:a) providing a test sample suspected of containing anti-rubella IgM antibodies; b) contacting the test sample with rubella antigens comprising rubella E1 glycoprotein and rubella E2 glycoprotein and substantially free of rubella capsid protein to form a binary complex, wherein the E1 and E2 glycoproteins are purified from rubella virus and wherein said binary complex is a complex of rubella antigens and IgM; c) contacting the binary complex with an indicator reagent to form a ternary complex; and d) detecting the presence or absence of the ternary complex; wherein said method specifically detects anti-rubella IgM antibodies and substantially eliminates false positive results.