Patent ID: 6218137
Filing Date: 2001-04-17
Classification: C07F,C12Q,Y10S

Abstract:
A method for detecting an analyte in a sample by a chemiluminescent assay procedure which comprises:(a) reacting a phosphatase enzyme with at least one compound of formula I to produce chemiluminescence for detecting the analyte, ##STR39##wherein in the compound of formula I, Het is a heterocyclic ring system comprising at least one five or six-membered ring which contains from 2 to 4 nitrogen atoms as heteroatoms, wherein z is selected from the group consisting of O and S atoms, wherein R.sub.6 is an organic group which allows chemiluminescence to be produced, wherein each M is independently selected from H and a cationic center and wherein n is a number which satisfies electroneutrality;(b) detecting the chemiluminescence; and(c) relating the amount of the chemiluminescence to the amount of the analyte.