Patent ID: 6846621
Filing Date: 2005-01-25
Classification: C07H,C12Q

Abstract:
1. A method for detecting the presence of an enterovirus in a sample comprising the steps of: (i) purifying RNA contained in the sample; (ii) reverse transcribing the RNA with primers effective to reverse transcribe enteroviral RNA to provide a cDNA; (iii) contacting at least a portion of the cDNA with (iv) carrying out an amplification procedure on the amplification mixture such that, if an enterovirus is present in the sample, an enterovirus amplicon is produced whose sequence comprises a nucleotide sequence of at least a portion of the VP1 gene of the enterovirus genome; and (v) detecting whether an amplicon is present; wherein the presence of the amplicon indicates that an enterovirus is present in the sample.