Patent ID: 8945865
Filing Date: 2015-02-03
Classification: C09B,G01N

Abstract:
1. A method of screening for a compound that enhances or inhibits a transport activity of OATP1B1, comprising: a step of preparing a cell expressing OATP1B1; a step of bringing dichlorofluorescein and a test compound into contact with the cell; a step of measuring a fluorescence intensity of dichlorofluorescein taken up by the cell; and a step of determining the test compound as a compound that enhances the transport activity of OATP1B1 in a case that the measured fluorescence intensity is higher than that when the test compound is not present and determining the test compound as a compound that inhibits the transport activity of OATP1B1 in a case that the measured fluorescence intensity is lower than that when the test compound is not present.