Patent ID: 6222020
Filing Date: 2001-04-24
Classification: A61K,C07K,C12Q

Abstract:
A non-naturally occurring antigen selected from the group consisting of(a) an immunogen comprising (i) a portion, at least five consecutive amino acids in length, of a tandem repeat sequence of the core protein of a human polymorphic epithelial mucin, said portion comprising an epitope of said core protein recognized by monoclonal antibody SM-3, secreted by hybridoma cell line HSM3 (accession number ECACC 87010701), and (ii) an immunogenic carrier, said immunogen also being recognized by SM-3,said portion (i) and said immunogenic carrier (ii) being covalently linked, directly or indirectly, so as to form said immunogen and(b) an antigenic fragment of the core protein of a human polymorphic epithelial mucin which fragment comprises at least five consecutive amino acids of a tandem repeat sequence of the core protein of a human polymorphic epithelial mucin, said fragment comprising an epitope of said core protein recognized by monoclonal antibody SM-3, said fragment also being recognized by SM-3,where said antigen does not occur in nature in normal or tumor cells, andwhere said antigen differs in amino acid sequence or glycosylation pattern from glycoproteins produced by normal and malignant human mammary epithelial cells and recognized by the monoclonal antibody HMFG-1.