Patent ID: 6538173
Filing Date: 2003-03-25
Classification: A01K,A61K,C12Q

Abstract:
A method of identifying a chromosomal locus containing at least one candidate gene involved in enhanced wound healing, comprising the steps of:(a) identifying a first and a second form of a polymorphic DNA microsatellite marker, wherein the first form identifies a first mouse strain and the second form identifies a second mouse strain, wherein the first mouse strain is a healer mouse strain and wherein the second mouse strain is not a healer mouse strain; (b) performing a first cross between a mouse of the first mouse strain and a first mouse of the second mouse strain to form F1 mice; (c) performing a second cross between (1) a first F1 mouse and (2) a mouse selected from the group consisting of (i) a second F1 mouse, (ii) a second mouse of the second mouse strain, and (iii) a second mouse of the first mouse strain; (d) identifying progeny of the second cross that exhibit an enhanced wound healing phenotype and progeny of the second cross that exhibit a non-enhanced wound healing phenotype; (e) identifying genotypes of progeny of the second cross, wherein the genotypes are selected from the group consisting of (1) a genotype homozygous for the first form of the polymorphic DNA microsatellite marker, (2) a genotype homozygous for the second form of the polymorphic DNA microsatellite marker, and (3) a genotype heterozygous for the first and second forms of the polymorphic DNA microsatellite marker; and (f) performing a statistical analysis to determine whether the first form of the polymorphic DNA microsatellite marker segregates with progeny of the second cross that exhibit the enhanced wound healing phenotype, whereby a chromosomal locus containing at least one candidate gene involved in enhanced wound healing is identified.