Patent ID: 8822643
Filing Date: 2014-09-02
Classification: C07K

Abstract:
1. Process for the purification of FV starting from human plasma or an FV-enriched intermediate fraction, wherein the protein is intact; said process comprising two chromatography steps on weak anion exchangers, wherein the first step is conducted in FV “non-binding” mode while the second step is in FV “capture” mode; wherein the two chromatography steps are separated by at least one viral inactivation step performed by solvent-detergent treatment; wherein after the second chromatography step, the FV containing solution is subjected to a viral removal step by nanofiltration; wherein cryosupernatant is used as starting material; wherein cryosupernatant is diluted with WFI to obtain a conductivity value comprised between 3 and 10 mS/Cm and supplemented with protease inhibitor Aprotinin and contacted with the first weak anion exchanger resin in a ratio that may vary from 0.15 to 2.0 g of dry resin per liter of diluted cryosupernatant.