Patent ID: 6773919
Filing Date: 2004-08-10
Classification: A01K,C12N

Abstract:
An vitro method of enhancing the transcription of a gene in a DNA construct when the DNA construct is incorporated into the genome of a eukaryotic host cell, the method comprising:(a) providing a DNA construct comprising a structural gene for a desired protein or polypeptide, a gene promoter upstream of and operably linked to the structural gene, and six copies of an enhancer element upstream of the promoter; (b) transfecting the eukaryotic host cell to incorporate the DNA construct into the genome of the host cell; and (c) exposing the DNA construct in the eukaryotic host cell to a hormone selected from the group consisting of lactogenic hormones, somatogenic hormones and mixtures thereof; wherein the enhancer element comprises the nucleotide sequence TTCTGAGAA, with the proviso that the nucleotide sequence does not contain the DNA sequence of nucleotide sequence SEQ ID NO:1, and wherein the enhancer element is responsive to both lactogenic hormones and somatogenic hormones.