Patent ID: 8497362
Filing Date: 2013-07-30
Classification: C07K,C12N

Abstract:
1. A method of selecting an isolated polynucleotide that affects the level of expression of a diacylglycerol acyltransferase polypeptide in a plant cell, the method comprising the steps of: (a) constructing a recombinant DNA construct comprising a promoter operably linked to an isolated polynucleotide comprising a nucleotide sequence of at least 30 contiguous nucleotides of SEQ ID NO:15; (b) introducing the recombinant DNA construct into a plant cell; (c) measuring the level of diacylglycerol acyltransferase polypeptide in the plant cell containing the recombinant DNA construct; (d) comparing the level of diacylglycerol acyltransferase polypeptide in the plant cell containing the recombinant DNA construct with the level of diacylglycerol acyltransferase polypeptide in a plant cell that does not contain the isolated polynucleotide; and (e) selecting for the isolated polynucleotide that affects the level of expression of the diacylglycerol acyltransferase polypeptide in the plant cell.