Patent ID: 8258082
Filing Date: 2012-09-04
Classification: C07K,C12N,C40B

Abstract:
1. A focused library of vectors or genetic packages, comprising variegated DNA molecules, each of which comprises a DNA sequence that encodes an antibody heavy chain variable region having a CDR1 region, a CDR2 region, and a CDR3 region, wherein the variegated DNA molecules collectively encode a plurality of antibody heavy chain variable regions comprising heavy chain CDR3 motifs of: xxxYCSSTSCxxx (SEQ ID NO:199), wherein each x independently is any amino acid or is absent; xxxxYVWGSYxxx (SEQ ID NO:200), wherein each x independently is any amino acid or is absent; xxxxxxxSGYxxx (SEQ ID NO:201), wherein each x independently is any amino acid or is absent; xxxYDILTGYYxx (SEQ ID NO:202), wherein each x independently is any amino acid or is absent; xxxVVVPAAxxxx (SEQ ID NO:203), wherein each x independently is any amino acid or is absent; xxxYYDSSGYxx (SEQ ID NO:204), wherein each x independently is any amino acid or is absent; xxxDFWSGxxxx (SEQ ID NO:205), wherein each x independently is any amino acid or is absent; xxxTIFGVxxxx (SEQ ID NO:206), wherein each x independently is any amino acid or is absent; xxxxIVATxxxx (SEQ ID NO:207), wherein each x independently is any amino acid or is absent; xxxYGSGSYYx (SEQ ID NO:208), wherein each x independently is any amino acid or is absent; xxxxYSYGxxx (SEQ ID NO:209), wherein each x independently is any amino acid or is absent; xxxCSGxxCYx (SEQ ID NO:210), wherein each x independently is any amino acid or is absent; xxxxAAAGxxx (SEQ ID NO:211), wherein each x independently is any amino acid or is absent; xGxxxGGNxxx (SEQ ID NO:212), wherein each x independently is any amino acid or is absent; xxxSGSYxxx (SEQ ID NO:213), wherein each x independently is any amino acid or is absent; xxxSSSWxxx (SEQ ID NO:214), wherein each x independently is any amino acid or is absent; xxxxTTVTTx (SEQ ID NO:215), wherein each x independently is any amino acid or is absent; xxxC(SG)GDxCx (SEQ ID NO:216), wherein each x independently is any amino acid or is absent; xxx(IV)AVAGxx (SEQ ID NO:217), wherein each x independently is any amino acid or is absent; xxLWFGELxx (SEQ ID NO:218), wherein each x independently is any amino acid or is absent; GxxWLxxxxF (SEQ ID NO:219), wherein each x independently is any amino acid or is absent; xxxDTxMVxx (SEQ ID NO:220), wherein each x independently is any amino acid or is absent; xxxxxGExxx (SEQ ID NO:221), wherein each x independently is any amino acid or is absent; xxxxSGWxx (SEQ ID NO:222), wherein each x independently is any amino acid or is absent; xxxxGYNxx (SEQ ID NO:223), wherein each x independently is any amino acid or is absent; xxxVRGVxx (SEQ ID NO:224), wherein each x independently is any amino acid or is absent; xxxIAAxxx (SEQ ID NO:225), wherein each x independently is any amino acid or is absent; xxYxWNxxx (SEQ ID NO:226), wherein each x independently is any amino acid or is absent; xxxYGDxx (SEQ ID NO:227), wherein each x independently is any amino acid or is absent; xxVGATxx (SEQ ID NO:228), wherein each x independently is any amino acid or is absent; and xxxYSSSx (SEQ ID NO:229), wherein each x independently is any amino acid or is absent.