Patent ID: 7507551
Filing Date: 2009-03-24
Classification: G01N

Abstract:
1. A method for measuring, in one assay, cholesterol in low density lipoprotein and total cholesterol in a biological sample, said method comprising: (i) introducing in said sample a first reagent that acts on the cholesterol in lipoproteins other than low density lipoprotein to generate a compound, and then measuring the absorbance of said compound; and subsequently (ii) introducing in the same sample from step (i) a second reagent that acts on at least the low density lipoprotein to generate an additional amount of said compound, and then measuring the elevated absorbance of said compound, wherein: (A) the first reagent has an HLB value of 13 or more and 15 or less and the second regent has an HLB value of 11 or more and 12 or less; and (B) the value from step (i) represents the amount of cholesterol in lipoproteins other than low density lipoprotein, the value from step (ii) represents the amount of total cholesterol in said sample, and the difference in values from step (i) and step (ii) represents the amount of cholesterol in low density lipoprotein.