Patent ID: 6280939
Filing Date: 2001-08-28
Classification: B82Y,C12Q,G01Q,Y10S

Abstract:
A method comprising:(A) incorporating nucleotides of more than one nucleotide type into a growing polynucleotide chain by a polymerase during a polymerase reaction in which the order of incorporation is driven by base pair rules and a delay occurs between each successive nucleotide incorporation;(B) controlling at least one characteristic of the incorporation of at least one nucleotide type of interest into the growing polynucleotide chain such that the concentration of a nucleotide of interest is different from the concentration of at least one other nucleotide and wherein the incorporation delay of the nucleotide of interest is different from the average incorporation delay for nucleotides of at least one other nucleotide type;(C) locating the polymerase with a local sensitive force detector;(D) detecting by using the local sensitive force detector, a motion associated with the nucleotide incorporation into the growing polynucleotide chain; and(E) producing data using a computer to transform detected incorporation data into nucleofide data.