Patent ID: 6312946
Filing Date: 2001-11-06
Classification: A61K,A61L,C07K,C12N

Abstract:
A replication defective recombinant adenovirus having an adenoviral genome, the genome comprising:a heterologous nucleic acid sequence which is operatively linked to a transcription promoter region that is functional in an infected cell,a first inactivated E1 region, wherein inactivation comprises a deletion or disruption in the E1 region,a second inactivated region, wherein inactivation is by deletion in a second essential region selected from the group consisting of a pIX-IVa2 region, an E4 region and an L5 region at its original location in the genome, andan insertion of a functional region at a position other than its original position, which complements the second inactivated region and which does not inactivate an additional essential region, wherein the functional region is all or part of an E4 region, a pIX-IVa2 region, or an L5 region,wherein the deletion in the second essential region does not inactivate an additional essential region which is not complemented by said insertion.