Patent ID: 7041452
Filing Date: 2006-05-09
Classification: C12Q

Abstract:
1. A method of identifying the grain varieties contained in a sample containing one or more unidentified grain varieties comprising: extracting DNA from the grain varieties contained in said sample; selectively amplifying said DNA by conducting multiplex PCR using the following primer pairs: SEQ ID NOs: 3 and 4; SEQ ID NOs: 19 and 20; SEQ ID NOs: 43 and 44; and SEQ ID NOs: 95 and 96; detecting absence or presences of bands corresponding to the amplification product for each of said primer pairs; and comparing the pattern of bands obtained from said detecting with the pattern of bands determined for a sample containing a known grain variety determined by selectively amplifying DNA obtained by multiplex PCR using the primer pairs: SEQ ID NOs: 3 and 4; SEQ ID NOs: 19 and 20; SEQ ID NOs: 43 and 44; and SEQ ID NOs: 95 and 96 thereby identifying the grain varieties contained in said sample.