Patent ID: 8003091
Filing Date: 2011-08-23
Classification: A61K,C12N

Abstract:
1. A pharmaceutical composition consisting essentially of (A) a biologically compatible medium that is isotonic and non-toxic and (B) intact bacterial minicells wherein said bacterial minicells are derived from S. typhimurium or E. coli , are approximately 0.4 μm in diameter, and are purified from parent bacterial cells in a sample enriched for minicells by a process that comprises filtering said sample with (a) at least one filter having a pore size of about 0.45 μm, such that minicells but not bacterial cells pass, and (b) at least one filter having a pore size of less than or equal to 0.2 μm, wherein said composition (i) contains fewer than about 1 contaminating parent bacterial cell per 10 9 minicells and (ii) is substantially free of cellular debris and endotoxin.