Patent ID: 9115178
Filing Date: 2015-08-25
Classification: C07K,C12Q,G01N

Abstract:
1. A method for separating and isolating at least one phosphorylated peptide or phosphorylated protein in a sample containing a mixture of peptides or proteins, the method comprising: loading the sample onto a mixed-mode chromatographic sorbent; eluting from the mixed-mode chromatographic sorbent in a first mode selected from the group consisting of reverse-phase mode and ion-exchange mode using a first eluent to produce a first eluate; eluting from the mixed-mode chromatographic sorbent in a second mode selected from the group consisting of reverse-phase mode and ion-exchange mode, the second mode being different from the first mode, using a second eluent to produce a second eluate; and isolating at least one phosphorylated peptide or phosphorylated protein analyte in the first eluate or the second eluate.