Patent ID: 6911327
Filing Date: 2005-06-28
Classification: B01L,C12Q,G01N

Abstract:
1. A method for determining an unknown starting quantity of a target nucleic acid sequence in a test sample, the method comprising the steps of: a) amplifying the unknown starting quantity of the target nucleic acid sequence in the test sample; b) amplifying a plurality of known starting quantities of a calibration nucleic acid sequence in respective calibration samples; c) determining a respective threshold value for each of the known starting quantities of the calibration nucleic acid sequence in the calibration samples and for the target nucleic acid sequence in the test sample, wherein the threshold value is determined for each nucleic acid sequence in a respective sample by; d) deriving a calibration curve from the threshold values determined for the known starting quantities of the calibration nucleic acid sequence in the calibration samples; and e) determining the starting quantity of the target nucleic acid sequence in the test sample using the calibration curve and the threshold value determined for the target sequence.