Patent ID: 6432657
Filing Date: 2002-08-13
Classification: G01N

Abstract:
A method for determining at least one coagulation parameter of a fluid sample comprising fibrinogen, said coagulation parameter being in a coagulation system comprising a coagulation process accompanied by limited degradation of fibrinogen with thrombin, said method comprising:A. contacting said fluid sample comprising fibrinogen with a blood coagulation reagent in the presence of a material which inhibits the formation of fibrin polymers from fibrin monomers in said coagulation process, said material being in an amount effective to avoid the formation of fibrin clot and fibrin net, B. detecting a degree of change in a physical value of said sample triggered by said contact, said physical value being selected from the group consisting of turbidity, viscosity, permittivity, and absorbance with a coexisting chromogenic substrate, and C. evaluating said degree of change in said physical value as an indicator to show the coagulation parameter, said coagulation parameter being at least one parameter(s) selected from the group consisting of (i) prothrombin time, (ii) activated partial thromboplastin time, (iii) fibrinogen concentration, (iv) thrombotest % activity, (v) hepaplastin test % activity, (vi) Protein C % activity, (vii) Protein S % activity, (viii) content of blood coagulation factor II, factor V, factor VII, or factor X, and (ix) content of blood coagulation factor VIII, factor IX, factor XI or factor XII.