Patent ID: 7238479
Filing Date: 2007-07-03
Classification: C12Q

Abstract:
1. A method for determining one or more alleles of the gene encoding micromolar calcium activated neutral protease effecting meat tenderness in a bovine animal, comprising: (1) assaying a sample of nucleic acids from a bovine for the presence of one or more single nucleotide polymorphisms in the bovine CAPN1 gene encoding micromolar calcium activated neutral protease, wherein said single nucleotide polymorphisms are selected from the group consisting of (a) a quanine or cytosine at position 18 of exon 9 of Seq. ID No. 3, (b) an adenine or quanine at position 17 of exon 14 of Seq. ID No. 4, and (c) a thymine or cytosine at position 185 on intron 19 of Seq. ID No. 4, and (2) selecting and removing from breeding bovine having a polymorphism selected from the group consisting of (i) a polymorphism at position 18 on exon 9 encoding glycine at amino acid 316 of said bovine micromolar calcium activated neutral protease, (ii) a polymorphism at position 17 on exon 14 encoding isoleucine at amino acid 530 of said bovine micromolar calcium activated neutral protease, and (iii) a polymorphism consisting of thymine at position 185 on intron 19.