Patent ID: 8425899
Filing Date: 2013-04-23
Classification: A61P,C12N

Abstract:
1. A method of treating a progressive myocardial injury that is an adverse consequence of a vascular insufficiency affecting coronary circulation of a subject, comprising (a) first, acquiring from the subject, under sterile conditions, a population of mononuclear cells comprising CD34+cells; (b) second, sterilely enriching the population of mononuclear cells for CD34+cells, so that at least 70% of the cells comprising CD34+cells; (c) third, at least 24 hours after step (a), passing a first portion of the enriched population produced in step (b) through a catheter and confirming that (d) fourth, formulating for parenteral delivery through a catheter a sterile pharmaceutical composition comprising: (e) fifth, administering the sterile pharmaceutical composition to the subject parenterally on a first infusion date; and (f) sixth, repeating steps (b)-(d), in order, and optionally step (a), at a plurality of infusion dates to treat ongoing vascular insufficiency affecting coronary circulation, thereby improving myocardial perfusion and preserving existing myocardiocytes and their function during the subject's lifetime.