Patent ID: 6539102
Filing Date: 2003-03-25
Classification: G01N,G06K,G16B

Abstract:
A method for creating a peptide and/or protein database comprising the steps of:providing a first source of peptides and/or proteins and providing a second source of peptides and/or proteins wherein the first source and the second source are from different tissues, cells, organelles or fractions of each; generating a 2-D separation of peptides and/or proteins of a first source; generating a 2-D separation of peptides and/or proteins of a second source; generating an electronic image of the 2-D separation of peptides and/or proteins of the first source; generating an electronic image of the 2-D separation of peptides and/or proteins of the second source; warping one of the electronic images of the 2-D separation of peptides and/or proteins to the other image; determining peptides and/or proteins common to both the sources after said warping one of the electronic images by analyzing the two 2-D separations of peptides and/or proteins of the first and second sources; characterizing a physical, chemical or biological property of the peptides and/or proteins previously determined to be common by a technique other than by the 2-D separation of peptides and/or proteins to establish protein identity, thereby confirming commonality of at least a portion of the peptides and/or proteins common to both of the 2-D separations of peptides and/or proteins; after said confirming, recording in a peptide and/or protein database all peptides and/or said proteins common to both sources as being the same in response to positive confirmation of the portion of the peptides and/or proteins common to both 2-D separations of peptides and/or proteins, thereby generating a database or part of a database of peptides and/or proteins common to both sources; analyzing peptides and/or proteins not common to both 2-D separations; and recording in the peptides and/or proteins database results of said analyzing the peptides and/or proteins not common to both 2-D separations, thereby generating a database or part of a database of peptides and/or proteins in one source and not in another source or in a significantly different amount in another source, wherein said proteins and/or peptides not common to both 2-D separations or in a significantly different amount in another source distinguishes one source from another source.