Patent ID: 6235522
Filing Date: 2001-05-22
Classification: A61K,C12N

Abstract:
A retroviral vector particle based on a first retrovirus, said retroviral vector particle capable of infecting and transducing non-dividing mammalian target cells, said retroviral vector particle comprising a packagable RNA genome capable of being inserted into a target cell genome when in the form of a DNA provirus, said RNA genome comprising sequences which provide in the DNA provirus:a) a non-lentiviral R region;b) a non-lentiviral expression control element located in the 5' long terminal repeat (LTR) of the provirus in place of the promoter function of the first retrovirus;c) a selected gene or genes under transcriptional control of the non-lentiviral expression control element in b), the selected gene or genes located between the LTRs; andwherein the RNA genome comprises essential lentiviral sequences for integration into the target cell genome.