Patent ID: 8394594
Filing Date: 2013-03-12
Classification: A61K,C07F,C07K,C12N,G01N

Abstract:
1. A method of detecting the presence of reactive antibody in the serum of a human comprising the steps of: a) contacting a sample of the serum obtained from the human with a suspension of red blood cells modified by incorporation of a water soluble peptide-lipid construct of the structure F-S-L to provide a mixture; b) adding an anti-human globulin antibody to the mixture; c) incubating the mixture from step a) for a time and at a temperature sufficient to allow agglutination; and then d) determining the degree of agglutination of the red blood cells in the mixture, wherein the degree of agglutination is indicative of the presence of the reactive antibody in said serum; where F is a peptide comprising an epitope for the reactive antibody, S is a spacer comprising an oligomer of ethylene glycol covalently linking F to L, and L is phosphatidylethanolamine, wherein the number of ethylene oxide repeats in said oligomer of ethylele glycol is 6 to 14.