Patent ID: 8361738
Filing Date: 2013-01-29
Classification: B01L,G01N

Abstract:
1. A method to detect an endogenous target in a sample, the method comprising: dividing the sample in a plurality of subsamples, each containing the endogenous target, adding to the subsamples set quantities of non-endogenous target and/or analog thereof, the subsamples comprising a subsample with no non-endogenous target nor analog thereof added thereto; detecting the added non-endogenous target or analog thereof in each subsample thus providing a set of detection signals comprising a detection signal related to the subsample with no non-endogenous target nor analog thereof added thereto; subtracting a background signal from each detection signal to provide a set of net signals comprising a net signal related to the subsample with no non-endogenous target nor analog thereof added thereto; providing a distribution of signal points, each signal point based on a net signal of the set of net signals, the signal points comprising a signal point related to the subsample with no non-endogenous target nor analog thereof added thereto, the distribution having a slope; and determining the endogenous target concentration in the sample by dividing the amplitude of the signal point related to the subsample with no non-endogenous target nor analog thereof added thereto by the slope of the distribution.