Patent ID: 9199002
Filing Date: 2015-12-01
Classification: A61F,A61L,A61P,C12N

Abstract:
1. A method of forming a hybrid soft tissue construct, the hybrid soft tissue construct comprising a core material, a biomaterial matrix, and isolated mammalian cells, the method comprising: providing the isolated mammalian cells, the biomaterial matrix and the core material; contacting the mammalian cells and the biomaterial matrix; contacting the biomaterial matrix and the core material; forming a plurality of macrochannels in the biomaterial matrix, the plurality of macrochannels not exposing the core material underlying the biomaterial matrix; and incubating the biomaterial matrix, the mammalian cells, and the core material to form the hybrid soft tissue construct; wherein, the biomaterial matrix is a polyethylene glycol diacrylate (PEGDA) hydrogel further comprising the mammalian cells; the hydrogel with the mammalian cells are contacted with the core material as a liquid then photopolymerized into a gel; formation of the macrochannels comprises placing capillary tubes radially at locations near the core material before the core material and the hydrogel with the mammalian cells are contacted; each of the capillary tubes is removed after the hydrogel with the mammalian cells contact the core material and after the hydrogel photopolymerizes into the gel; the core material is acellular and biocompatible, and designed to remain acellular upon implantation in a subject; the biomaterial matrix comprises a plurality of microchannel pores throughout the biomaterial matrix; the plurality of macrochannels have an average diameter of 0.1 mm to 50 mm; the plurality of macrochannels does not expose the core material to host tissue; and the biomaterial matrix contacts and covers the acellular core.