Patent ID: 8735149
Filing Date: 2014-05-27
Classification: C12N

Abstract:
1. A method for directing terminal differentiation of neural precursors into motor neurons comprising: providing a population of neural precursors; culturing the neural precursors in a first culturing medium comprising a basic medium supplemented with at least hedgehog (HH) or an HH agonist and with retinoic acid (RA) to obtain neural progenitors having a caudal and ventral specification that is exhibited by at least expression of Ngn2 after obtaining the neural progenitors having a caudal and ventral specification, culturing the neural progenitors having a caudal and ventral specification in a second culturing medium comprising a basic medium supplemented with at least one inhibitor of the Notch signaling pathway, and allowing the neural progenitors having a caudal and ventral specification to terminally differentiate into postmitotic motor neurons expression HB9.