Patent ID: 6218136
Filing Date: 2001-04-17
Classification: A61K,A61P,C07K,C12N,C12Q,G01N

Abstract:
A method for identifying at lest one compound as a mitogen activated protein kinase-activated protein (MAPKAP) kinase-3 inhibitor, said method comprising the steps of:a. incubating purified, or enriched, active MAPKAP kinase-3 (SEQ ID NO:2) or a mixture of unactivated MAPKAP kinase-3 (SEQ ID NO:2) and active cytokine suppressive anti-inflammatory drug binding protein (CSBP) with peptide or protein substrate in the presence of labelled ATP, and said at least one compound;b. measuring the incorporation of the labelled phosphorus into the peptide or protein substrate; andc. identifying a compound that reduces the amount of labelled phosphorus included in said substrate.