Patent ID: 8772048
Filing Date: 2014-07-08
Classification: C07D,Y10S

Abstract:
1. A label comprising: a fluorophore covalently linked to a biomolecule, wherein said biomolecule comprises a DNA, RNA, monosaccharide, polysaccharide, nucleotide (ATP, GTP, cAMP), lipid, peptide, or a protein including enzyme and other structural proteins, or any combination thereof; wherein said fluorophore is derived from a photoactivatable fluorogen, said photoactivatable fluorogen comprising an azide conjugated covalently to an acceptor group through a pi-conjugated system (pi-bridge); wherein said fluorophore is derived from said photoactivatable fluorogen by a photochemical conversion whereby said azide group is converted to an amine donor group and wherein said fluorophore comprises said amine donor group conjugated covalently to said acceptor group through said pi-bridge so that an electron from the amine donor can be delocalized to said acceptor group through said pi-bridge; wherein said pi-bridge of said fluorogen is a conjugated system and is derived from one or more contiguous alkenes each, independently, containing from 2 to about 12 carbon atoms, or wherein said one or more contiguous alkenes is a replaced —(CH═N)— unit, or wherein said one or more contiguous alkenes are substituted with an aliphatic group containing from 1 to about 18 carbon atoms; or is derived from one or more alkynes each, independently, containing from 2 to about 12 carbon atoms; or is derived from one or more aromatic or heterocyclic aromatic or mono or polysubstituted aromatic or heterocyclic wherein each, independently, aromatic group has from 3 to about 26 total ring atoms, or each, independently, heterocyclic aromatic group has from 3 to 26 total ring atoms and from 1 to 3 nitrogen, oxygen, sulfur, phosphorus, or selenium atoms, and wherein each substituent, independently, is a) one or more alkyl groups having a total of from 1 to about 18 carbon atoms, or b) is one or more alkene groups having a total of from 2 to about 4 carbon atoms, or c) one or more alkyne groups having a total of from 2 to 10 carbon atoms; or d) is an unsubstituted heterocyclic group having a total of from 3 to about 26 carbon atoms, and, independently, from 1 to 3 nitrogen, oxygen, sulfur, phosphorus, or selenium atoms; or e) wherein said substituent is one or more halides; or f) wherein said substituent can be connected to 2 or more of said aromatic groups; or any combination of the preceding; wherein said acceptor group of said fluorophore is where R or any combination thereof, and wherein said R wherein said pi-bridge of said fluorophore is the same as said pi-bridge of said fluorogen, and wherein said acceptor group of said fluorophore is the same as said acceptor group of said fluorogen; and wherein said fluorophore is excitable at a wavelength of greater than about 440 nanometers.