Patent ID: 6177263
Filing Date: 2001-01-23
Classification: C12N,C12Q

Abstract:
A method of recombining polynucleotides to produce recombinant polynucleotides, comprising:(a) providing a population of different template polynucleotides having different sequences and a pair of first and second primers respectively disposed to hybridize to at least one strand of the template polynucleotides and its complement;(b) conducting a multi-cyclic polynucleotide extension reaction on the template polynucleotides primed from the first primer and not the second primer under conditions of incomplete extension, whereby incomplete extension product primed from the first primer anneals to different members of the population of template polynucleotides having different sequences in different extension cycles and undergoes further extension thereby generating recombinant incomplete extension products and subsequently full-length recombinant extension products;(c) contacting the full-length recombinant extension products with the second primer and extending the second primer to form double stranded full-length recombinant extension products.