Patent ID: 8986743
Filing Date: 2015-03-24
Classification: A01K

Abstract:
1. A method for producing human immune-competent cells in a genetically immunodeficient mouse, wherein the mouse is a NOD mouse or a NOD/SCID mouse, the method comprising: a) transplanting an amount of human mononuclear cells (MNC) into the genetically immunodeficient mouse, and b) administering to the genetically immunodeficient mouse a conditioned cell culture supernatant of a culture of at least one member of the group consisting of human cell lines, cells and tissue; wherein the conditioned cell culture supernatant contains the factors: interleukin-8 (IL-8), interleukin-6 (IL-6), vascular endothelial growth factor (VEGF), interferon-γ-induced protein 10 (IP-10), monocyte chemoattractant protein-1 (MCP-1), angiogenin (ANG) and tissue-type plasminogen activator (t-PA) , each of the factors contained in a concentration of 20 pg/ml to 10 ng/ml, wherein 8 weeks after the transplantation of said MNC, said immunodeficient mouse produces T-cells and B-cells, monocytes, dendritic cells, NK cells, granulocytes, and CD34+ stem cells, wherein the amount of human MNC is sufficient to establish at least 29% T-cells and 20% B-cells of human origin in the spleen of the mouse.