Patent ID: 6440672
Filing Date: 2002-08-27
Classification: A61K,C07K,C12Q,Y02A

Abstract:
A method for identifying an inhibitor compound useful for inhibiting mucin production comprising:(a) contacting cells comprising a mucomone-inducible reporter gene construct with a mucomone, wherein said reporter gene construct comprises a mucomone response element and a responsive promoter, operably linked to a reporter gene, wherein said mucomone response element comprises a polynucleotide molecule selected from the group consisting of a polynucleotide molecule having the sequence of SEQ ID NO: 1 from nucleotide 1 to nucleotide 218, apolynucleotide molecule having the sequence of SEQ ID NO: 1 from nucleotide 2392 to nucleotide 3752, and a polynucleotide molecule having the sequence of SEQ ID NO: 1 from nucleotide 3139 to nucleotide 3752, and complements thereof; (b) contacting said cells with a test compound, and comparing the reporter gene activity after performing steps (a) and (b) with the reporter gene activity after performing only step (a), wherein a decrease in reporter gene activity after performing steps (a) and (b) compared to reporter gene activity after performing only step (a) is correlated to inhibition of mucin production induced by said mucomone.