Patent ID: 6713251
Filing Date: 2004-03-30
Classification: C12Q

Abstract:
A kit for inferring the nucleotide sequence at codons of interest in the HIV reverse transciptase (RT) and/or the amino acids corresponding to these codons and/or the HIV RT resistance spectrum of HIV isolates present in a biological sample comprising:(i) optionally, a means for releasing, isolating, or concentrating the polynucleic acids present in said sample; (ii) optionally, at least one suitable set of primers; (iii) at least two different probes, wherein each probe is capable of hybridizing specifically to one or more target codons within any region I to VIII as represented in FIG. 1, said probes optionally fixed to a solid support; (iv) a hybridization buffer, or components necessary for producing said buffer; (v) a wash solution, or components necessary for producing said solution; (vi) optionally, a means for detecting the hybrids resulting from the preceding hybridization; (vii) optionally, a means for attaching said probe to a solid support wherein the set of primers is selected from the group consisting of: SEQ ID No: 162 and 163, and SEQ ID No: 164 and 39.