Patent ID: 6350604
Filing Date: 2002-02-26
Classification: C11D,C12N

Abstract:
An isolated lipolytic enzyme, which is:a) a polypeptide encoded by the DNA sequence cloned into plasmid pYES 2.0 present in Escherichia coli deposited as DSM 10591, DSM 10590 or DSM 11298; b) a polypeptide having an amino acid sequence as shown in positions 1-200 of SEQ ID NO: 3, positions 1-202 of SEQ ID NO: 6, or positions 1-201 of SEQ ID NO: 8; or c) a polypeptide encoded by DNA that hybridizes under conditions of hybridization in 5 times SSC, 5 times Denhardt's solution, 0.5% SDS and 100 &mgr;g/ml of denatured sonicated salmon sperm DNA at 45Â° C. and washing in 2 times SSC, 0.5% SDS at 55Â° C. with: i) a DNA sequence cloned into plasmid pYES 2.0 present in Escherichia coli deposited as DSM 10591, DSM 10590 or DSM 1 1298; or ii) a DNA sequence shown in positions 114-713 of SEQ ID NO: 2, positions 133-738 SEQ ID NO: 5 or positions 161-763 of SEQ ID NO: 7.