Patent ID: 6811999
Filing Date: 2004-11-02
Classification: A61K,A61P,C12Q,Y10S

Abstract:
A method of determining calciotropic activity of a preparation of algae of the genus Padina pavonica, said preparation selected from the group consisting of an extract of said algae and a powder of said algae, said method consisting of culturing a human or animal osteoblasts in culture medium rich in calcium ions in a well plate, adding to said culture medium said extract or powder of said algae Padina pavonica, thereafter eliminating non-fixed calcium ions in the culture medium, acidifying the culture medium to destroy extracellular matrix formed by the osteoblasts, thereafter filtering the culture medium, and then determining a concentration of fixed calcium ions in the osteoblasts in comparison with a calibration scale of a known activator of the fixation of calcium or in the presence of an inhibitor of the fixation of calcium or both.