Patent ID: 6013764
Filing Date: 2000-01-11
Classification: A61K,C07K

Abstract:
A process for producing the synthetic KL-4 pulmonary surfactant protein of the formula (Lys-Leu.sub.4).sub.4 Lys (SID No. 1) comprising the steps of:a. reacting a 3-amino acid peptide residue of the formula H-Lys(Z)-Leu-Leu OH with a 5-amino acid peptide residue Boc-Leu-Leu-Lys(Z)-Leu-Leu-OH (SID No. 2) to yield an 8-amino acid peptide residue, which is successively reacted with the 5-amino acid peptide to form an 18-amino acid peptide of the formula H-Leu-Leu(Lys(Z)-Leu.sub.4).sub.3 Lys(Z)-OBzl (SID No. 3) wherein Z is benzyloxycarboxyl;b. reacting the 18 amino acid peptide residue with a 3-residue amino acid peptide of the formula: H-Leu-Leu-Lys(Z)-OBzl to form the protected 21-amino acid KL-4 protein; andc. removing the protecting group of the 21-amino acid KL-4 protein by reaction with a suitable acid to form the final KL-4-protein.