Patent ID: 8871730
Filing Date: 2014-10-28
Classification: A61K,A61P,C12N

Abstract:
1. A small hairpin RNA (shRNA) consisting of: an antisense sequence consisting of about 16 to about 19 nucleotides; a sense sequence consisting of about 11 to about 19 nucleotides, wherein the sense sequence is substantially complementary to the antisense sequence; a loop region connecting the antisense and sense sequences; and optionally, an overhang region, a conjugate moiety, and/or a detectable label, wherein the loop region comprises at least one of the following chemical modifications: 2′-H, 2′-O-alkyl, or 2′-halo modification of one or more nucleotides or one or more phosphorothioate modifications of the backbone; wherein the at least one chemical modification confers reduced immunostimulatory activity, increased serum stability, or both, as compared to a corresponding shRNA not having the chemical modification.