Patent ID: 6090602
Filing Date: 2000-07-18
Classification: A61K,C07H

Abstract:
A method of inhibiting hybridization of a reverse-transcribing DNA strand with an RNA template comprising the steps of:delivering at least one nucleoside analog containing an L-rhamnose moiety to the RNA template, wherein the nucleoside analog is selected from the group consisting of 1'-adenyl-L-rhamnose, 1'-cytodinyl-L-rhamnose, 1'-guanyl-L-rhamnose, 1'-thymidyl-L-rhamnose, and 1'-uridinyl-L-rhamnose;wherein the nucleoside analog is phosphorylated into a nucleotide to form a nucleotide analog;wherein the nucleotide analog is incorporated into DNA synthesized during reverse transcription of the RNA template; andwherein hybridization between the reverse-transcribed DNA and the RNA template is inhibited by the presence of the incorporated nucleotide analog.