Patent ID: 7153656
Filing Date: 2006-12-26
Classification: C12Q

Abstract:
1. A method for identifying the presence of a specific nucleotide sequence in a sample nucleic acid, comprising: (a) hybridizing to the sample nucleic acid a pair of oligonucleotides capable of ligating together when hybridized to the sample nucleic acid if the specific nucleotide sequence is present therein, wherein one oligonucleotide further comprises a tail sequence containing a first PCR primer sequence and the other oligonucleotide further comprises a tail sequence containing the complement of a second PCR primer sequence, and wherein at least one of the oligonucleotides contains a polynucleotide capture sequence; (b) PCR amplifying the oligonucleotides with first and second PCR primers corresponding to the first and second PCR primer sequences, wherein at least one of the primers is labeled, to generate a PCR amplification product; and, (c) contacting the PCR amplification product with a microsphere linked to a polynucleotide address tag sequence complementary to the capture tag sequence, wherein the presence of the specific nucleotide sequence is identified by the detection of a labeled amplicon corresponding to a ligated oligonucleotide complex, and wherein the labeled amplicon is detected by optically detecting the microsphere.