Patent ID: 8222040
Filing Date: 2012-07-17
Classification: C12Q,G01N,G06K,Y10T

Abstract:
1. A method for sequencing nucleic acid microparticles, the method comprising the steps of: selectively exciting a calibration target by interference of a plurality of illumination beams; obtaining optical images of the excited calibration target; processing the optical images of the excited calibration target to generate a first optical image of the excited calibration target; predicting a second optical image of the excited calibration target based on first calibration parameters; generating second calibration parameters based on a difference between the first optical image and the second optical image; performing a sequencing reaction on the nucleic acid microparticles using one or more sequencing reagents, the nucleic acid particles being different from the calibration target; selectively exciting the nucleic acid microparticles in an excitation pattern using the second calibration parameters; obtaining optical images of the nucleic acid microparticles at a resolution insufficient to resolve individual microparticles; and processing the optical images of the nucleic acid microparticles using information on the excitation pattern to determine a presence or absence of at least one optical signature, the presence or absence of said at least one optical signature indicating sequence information of the nucleic acid microparticles.