Patent ID: 6172203
Filing Date: 2001-01-09
Classification: A61K,C07K

Abstract:
A method for purifying exogenous pulmonary surfactant comprising the steps of:washing bronchio alveoli with 0.8-1.2% p/v saline solution thereby forming a first solution;fractionating the first solution originated in the washing step by differential centrifugation, whereby a first pellet is produced;resuspending the first pellet in a buffer and further fractionating it by first centrifugation whereby a second pellet is produced;resuspending the second pellet in a polar solvent and fractionating the resultant second solution by a second centrifugation whereby a third pellet is produced;dissolving said third pellet in a water-insoluble second solvent forming a third solution;extracting said third solution with a hypertonic saline solution; andcollecting the bottom liquid phase resulting from said extraction.