Patent ID: 7138112
Filing Date: 2006-11-21
Classification: A61K,A61P,C07K,C12N,Y02A

Abstract:
1. A method for eliciting an immune response in a subject comprising administering a live attenuated bacterial vector vaccine to said subject, wherein the live attenuated bacterial vector vaccine comprises an isolated cell comprising an expression vector, wherein said expression vector comprises a nucleotide sequence encoding: a restricted-copy-number origin of replication cassette comprising (i) a nucleotide sequence encoding an origin of replication that limits the expression vector to an average plasmid copy number of about 2 to 75 copies per cell, (ii) a first unique restriction enzyme cleavage site located 5′ of the nucleotide sequence encoding the origin of replication, and (iii) a second unique restriction enzyme cleavage site located 3′ of the nucleotide sequence encoding the origin of replication; at least one post-segregational killing cassette comprising (i) a nucleotide sequence encoding at least one post-segregational killing locus, (ii) a first unique restriction enzyme cleavage site located 5′ of the nucleotide sequence encoding the at least one post-segregational killing locus, and (iii) a second unique restriction enzyme cleavage site located 3′ of the nucleotide sequence encoding the at least one post-segregational killing locus, wherein said nucleotide sequence encoding at least one post-segregational killing locus encodes the ssb post-segregational killing locus; and at least one partitioning cassette comprising (i) a nucleotide sequence encoding at least one partitioning function, (ii) a first unique restriction enzyme cleavage site 5′ of the nucleotide sequence encoding the at least one partitioning function, and (iii) a second unique restriction enzyme cleavage site located 3′ of the nucleotide sequence encoding the at least one partitioning function.