Patent ID: 8932811
Filing Date: 2015-01-13
Classification: C12Q,Y10T

Abstract:
1. A method for determining the sequence of a target nucleic acid molecule, comprising: providing two capture oligonucleotide probes with different sequences, each capture oligonucleotide probe including a 5′ and a 3′ end and one of a plurality of differently encoded microcarriers attached to the 5′ end, wherein the encoded microcarrier attached to each capture oligonucleotide probe includes a code corresponding with the sequence of the capture oligonucleotide probe and its location on a reference genome which is known; hybridizing the capture oligonucleotide probes with a sample comprising the target nucleic acid molecule, wherein the target nucleic acid molecule includes a 5′ and a 3′ end and comprises two separate regions for binding the capture oligonucleotide probes, the separate regions having sequences which are at least partially complementary to the sequences of the capture oligonucleotide probes; determining the sequence of the hybridized target nucleic acid molecule from the 3′ end of the capture oligonucleotide probes towards the 5′ end of the target nucleic acid by using the capture oligonucleotide probes as primers and generating a sequence read from each capture oligonucleotide probe of at least 2 nucleotides using a DNA polymerase; determining the codes on the plurality of differently encoded microcarriers; identifying the nucleotide sequence of the capture oligonucleotide probes corresponding with the code on the encoded microcarrier attached to the capture oligonucleotide probes and the locations of the capture oligonucleotide probes on the reference genome; and combining the sequence information from the target hybridized nucleic acid molecule with the sequence information from the capture oligonucleotide probes to determine the sequence of the target nucleic acid molecule.