Patent ID: 8951755
Filing Date: 2015-02-10
Classification: A23L,A61K,A61Q,C07K,C11D,C12N

Abstract:
1. A method of producing a composition containing active VHH antibodies, the method comprising: (a) generating a binding domain display library comprising nucleic acid molecules encoding VHH antibodies, wherein binding domains of the VHH antibodies capable of binding antigens are presented on the surface of the library; (b) selecting for binding domain display library members wherein the VHH antibodies presented on the surface of the library retain binding activity at pH value of less than 5 or more than 9; (c) expressing VHH antibodies selected for in step (b) using nucleic acid encoded by binding domain display library members; and (d) adding the VHH antibodies expressed in (c) into the composition.