Patent ID: 8728804
Filing Date: 2014-05-20
Classification: C12N,C12Y

Abstract:
1. A nucleic acid construct or expression vector, comprising a polynucleotide encoding a protein complex having succinyl-CoA:acetoacetate transferase activity operably linked to one or more heteroloqous control sequences that direct the production of the protein complex in an expression host, wherein the complex comprises a first polypeptide subunit selected from: (a) a polypeptide having at least 97% sequence identity to SEQ ID NO: 3; (b) a polypeptide encoded by a polynucleotide that hybridizes under at least very high stringency conditions with the full-length complementary strand of SEQ ID NO: 1 or 2; and (c) a polypeptide encoded by a polynucleotide having at least 97% sequence identity to SEQ ID NO: 1 or 2; and a second polypeptide subunit selected from: (a) a polypeptide having at least 97% sequence identity to SEQ ID NO: 6; (b) a polypeptide encoded by a polynucleotide that hybridizes under at least very high stringency conditions with the full-length complementary strand of SEQ ID NO: 4 or 5; and (c) a polypeptide encoded by a polynucleotide having at least 97% sequence identity to SEQ ID NO: 4 or 5.