Patent ID: 6680166
Filing Date: 2004-01-20
Classification: A61M,C12M

Abstract:
A method for culturing cells comprising an extracorporeal circuit comprising the steps of:a. seeding a cell culture unit comprising: i. a shell having two ends and defining an elongated chamber there between; ii. a liquid and a gas perfusion port located on each of said end; iii. liquid perfusion fibers connected to said liquid perfusion ports and gas perfusion fibers connected to said gas perfusion ports; said fibers defining extracapillary and intracapillary space; iv. communicating with the intracapillary space of said gas perfusion fibers; v. means communicating wit the intracapillary space of said liquid perfusion fibers; and vi. means communicating with said extracapillary space; with cells; b. withdrawing blood from a patient; c. pumping said withdrawn blood through the liquid perfusion ports of said culture unit while supplying oxygen through said gas perfusion ports; d. returning said filtered blood to the patient; and e. extracting cells or cellular components.