Patent ID: 9181535
Filing Date: 2015-11-10
Classification: C12N

Abstract:
1. A polynucleotide sequence encoding a transcription activator like effector nuclease (TALEN), the TALEN comprising from an N-terminus to a C-terminus: (i) a first segment of about 50 to about 200 amino acids in length, wherein the first segment comprises the amino acid sequence of SEQ ID NO: 1; (ii) a TAL effector DNA-binding domain providing sequence-specific binding to a target nucleotide sequence, wherein the TAL effector DNA-binding domain comprises 12-31 TAL repeats and a C-terminal truncated TAL repeat; (iii) a second segment of about 20 to 100 amino acids in length, wherein the first segment comprises the amino acid sequence of SEQ ID NO: 2; and (iv) a modified FokI nuclease catalytic domain.