Patent ID: 7300784
Filing Date: 2007-11-27
Classification: C12N,C12P

Abstract:
1. An isolated protein complex consisting of an α-subunit protein and a β-subunit protein, wherein said protein complex is capable of converting: a nitrile group of a nitrile compound to an amide group; and wherein the α subunit protein is a protein selected from the group consisting of: (A) a protein comprising the amino acid sequence of SEQ ID NO: 2; (B) a protein comprising the amino acid sequence of SEQ ID NO: 2 in which one to ten amino acids are substituted, deleted, inserted and/or added; (C) a protein having 95% or higher identity to the amino acid sequence of SEQ ID NO: 2; (D) a protein encoded by a polynucleotide comprising the nucleotide sequence of SEQ ID NO:1; and (E) a protein encoded by a polynucleotide that hybridizes to the nucleotide sequence of SEQ ID NO:1 under stringent conditions of 6×SSC at about 45° C., followed by one or more washes in 0.2×SSC, 0.1% SDS at 65° C.; and wherein the β subunit protein is a protein selected from the group consisting of: (a) a protein comprising the amino acid sequence of SEQ ID NO: 4; (b) a protein comprising the amino acid sequence of SEQ ID NO: 4 in which one to ten amino acids are substituted, deleted, inserted and/or added; (c) a protein having 95% or higher identity to the amino acid sequence of SEQ ID NO: 4; (d) a protein encoded by a polynucleotide comprising the nucleotide sequence of SEQ ID NO:3; and (e) a protein encoded by a polynucleotide that hybridizes to the nucleotide sequence of SEQ ID NO:3 under stringent conditions of 6×SSC at about 45° C., followed by one or washes in 0.2×SSC, 0.1% SDS at 65° C.