Patent ID: 6149919
Filing Date: 2000-11-21
Classification: A61K,A61P,C07K

Abstract:
An immunogenic detoxified protein comprising an amino acid sequence of subunit A of a cholera toxin or a fragment thereof or an amino acid sequence of subunit A of an Escherichia coli heat labile toxin or a fragment thereof comprising one or more of the following amino acid replacements at or in positions corresponding to amino acids in the sequence of subunit A of mature cholera toxin: Val-53-Asp, Val-53-Glu, Val-53-Tyr, Ser-63-Lys, Val-97-Lys, Val-97-Tyr, Tyr-104-Lys, Tyr-104-Asp, Tyr-104-Ser, and Pro-106-Ser, wherein said fragment comprises from 3 to about 5 amino acids of at least one of the regions containing one of the amino acids to be replaced.