Patent ID: 8404454
Filing Date: 2013-03-26
Classification: C07K,G01N

Abstract:
1. A method for identifying a T-cell receptor (TCR) protective against a disease, which comprises the following steps: i) obtaining T cells from a donor non-human animal which comprises T cells protective against the disease amongst its complete T cell repertoire; ii) adoptive transfer of the T cells into a plurality of T-cell deficient recipient non-human animals in a number such that, following subsequent disease challenge, at least one recipient animal is protected against the disease but at least one animal remains un-protected; iii) analyzing TCRVβ sequences of TCR(s) present in the at least one protected and the at least one unprotected animals; and iv) identifying a TCR protective against the disease by identifying TCRVβ sequence(s) present in the at least one protected animal and not present in the at least one unprotected animal.