Patent ID: 6348321
Filing Date: 2002-02-19
Classification: C07K,C12N

Abstract:
A method for identifying an agent which is an inhibitor of fatty acid uptake by a Fatty Acid Transport Protein (FATP), said FATP encoded by a polynucleotide comprising a nucleotide sequence which encodes a protein consisting of the amino acid sequence of SEQ ID NO:49, comprising the steps of:a) maintaining test cells expressing said polynucleotide in the presence of a fatty acid and an agent to be tested as an inhibitor of fatty acid uptake; b) measuring uptake of the fatty acid in the test cells; and c) comparing uptake of the fatty acid in the test cells with uptake of the fatty acid in suitable control cells; wherein lower uptake of the fatty acid in the test cells compared to uptake of the fatty acid in the control cells is indicative that the agent is an inhibitor of fatty acid uptake by said protein.