Patent ID: 8906628
Filing Date: 2014-12-09
Classification: C12Q

Abstract:
1. An assay for the detection of E. coli O157:H7 in a sample comprising: a) hybridizing a first pair of PCR primers to a first target polynucleotide sequence within SEQ ID NO:111; b) hybridizing a second pair of PCR primers to a second target polynucleotide sequence within a sequence selected from SEQ ID NO:91-110; c) amplifying said at least first and said at least second target polynucleotide sequences; and d) detecting said at least first and said at least second amplified target polynucleotide sequence products; wherein the detection of the at least first amplified target polynucleotide sequence product and the detection of the at least second amplified target polynucleotide sequence product is indicative of the presence of