Patent ID: 7501251
Filing Date: 2009-03-10
Classification: B01J,B01L,C07B,C07F,C07H,C12Q,C40B,G01N,H01J

Abstract:
1. A method for detecting the presence or absence of a target nucleic acid in a biological sample using RNA amplification, comprising: a. amplifying a target nucleic acid, or portion thereof, using a primer comprising a sequence that is complementary to a polynucleotide sequence in the target nucleic acid, or a complement thereof, and a sequence that encodes an RNA polymerase promoter; b. synthesizing RNA using an RNA polymerase that recognizes the promoter when the target nucleic acid is present; and c. detecting the presence or absence of the resulting RNA using mass spectrometry thereby detecting the presence or absence of the target nucleic acid in the biological sample.