Patent ID: 7824897
Filing Date: 2010-11-02
Classification: C07K,C12N

Abstract:
1. A method for obtaining a crystal comprising a tumor necrosis factor-alpha (TACE): ligand complex between a substitute ligand and a modified TACE catalytic domain comprising: incubating an excess of a substitute ligand with a crystal comprising a modified TACE catalytic domain complexed to an initial ligand, wherein said modified TACE catalytic domain comprises SEQ ID NO: 8 and said crystal forms in space group P2 catalyzes the proteolytic cleavage of SEQ ID NO: 17; and wherein said incubating is performed under appropriate conditions and for a sufficient time period for the substitute ligand to replace the initial ligand in the protein-ligand complex; and wherein a crystal comprising the protein-ligand complex between the substitute ligand and the modified TACE catalytic domain is obtained.