Patent ID: 6747273
Filing Date: 2004-06-08
Classification: C12Q,G01N,Y10T

Abstract:
A method for identifying the structure of a dimethylarginine comprising:(i) obtaining, by mass spectroscopy, a neutral loss spectrum of a peptide containing a dimethylarginine, and (ii) ascertaining if the neutral loss spectrum shows one or both of neutral loss of monomethylamine (MMA), dimethylcarbodiimide (DMC), and/or neutral loss of dimethylarginine (DMA), wherein neutral loss of monomethylamine (MIMA) and of dimethylcarbodiimide (DMC) indicates the presence of a symmetrically dimethylated arginine residue, whereas neutral loss of dimethylamine (DMA) indicates the presence of an asymmetrically dimethylated arginine residue.