Patent ID: 7279463
Filing Date: 2007-10-09
Classification: C12N

Abstract:
1. A method for targeted recombination of a nucleic acid molecule to produce a change in the genome of an intact human or animal comprising the steps of: a) providing a single-stranded oligonucleotide having a sequence that forms a triple-stranded nucleic acid molecule by hybridizing with a target sequence in a double-stranded nucleic acid molecule with a Kd of less than or equal to 2×10 b) providing a donor nucleic acid such that recombination of the donor nucleic acid into the target sequence is induced by triple helix formation between the single-stranded oligonucleotide and the double-stranded nucleic acid molecule; and c) administering the single-stranded oligonucleotide into an intact human or animal having a sequence that forms a triple-stranded nucleic acid molecule with the target sequence located in the genome of the intact human or animal, wherein the oligonucleotide binds to the target sequence with a Kd of less than or equal to 2×10