Patent ID: 7629150
Filing Date: 2009-12-08
Classification: A01K,C07K,C12N,C12Y

Abstract:
1. A method for producing a cell having increased activity of a beta 1,3-N-acetylglucosaminyltransferase, which comprises the steps of: introducing DNA or RNA into a cell to express a polypeptide encoded by the DNA or RNA, wherein said DNA or RNA is selected from the group consisting of the following (a) to (g): (a) a DNA encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:1 and having a beta 1,3-N-acetylglucosaminyltransferase activity, (b) a DNA encoding a polypeptide comprising an amino acid sequence of positions 39-378 in the amino acid sequence of SEQ ID NO:1 and having a beta 1,3-N-acetylglucosaminyltransferase activity, (c) a DNA encoding a polypeptide comprising an amino acid sequence having 95% or more homology with the amino acid sequence in the polypeptide according to (a) or (b), and having a beta 1,3-N-acetylglucosaminyltransferase activity, (d) a DNA comprising the nucleotide sequence of SEQ ID NO:2 and encoding a polypeptide having a beta 1,3-N-acetylglucosaminyltransferase activity, (e) a DNA comprising the nucleotide sequence of positions 135 to 1268 of SEQ ID NO:2 and encoding a polypeptide having a beta 1,3-N-acetylglucosaminyltransferase activity, (f) a DNA comprising the nucleotide sequence of positions 249 to 1268 of SEQ ID NO:2 and encoding a polypeptide having a beta 1,3-N-acetylglucosaminyltransferase activity, and (g) an RNA comprising a sequence corresponding to the DNA selected from any one of (a) to (g); and culturing the cells in order to express said polypeptide.