Patent ID: 6485925
Filing Date: 2002-11-26
Classification: C12N,C12Q,G01N

Abstract:
An in vivo method for screening modulators of lethal factor (LF) mitogen activated protein kinase kinase (MAPKK) protease activity, the method comprising the steps of:(i) contacting a living cell with LF, wherein the LF has MAPKK protease activity; (ii) contacting the cell with substances suspected of having the ability to modulate MAPKK protease activity; (iii) assaying for the level of LF MAPKK protease activity; (iv) assaying for the level of LF MAPKK protease activity in the absence of said substances suspected of having the ability to modulate LF MAPKK protease activity; and (v) comparing the LF MAPKK protease activity in step (iii) to that in step (iv), whereby substances are identified as modulators when the LF MAPKK activity in step (iii) is lower than the LF MAPKK activity in step (iv).