Patent ID: 6265175
Filing Date: 2001-07-24
Classification: A61K,A61P,C12M,C12N

Abstract:
A method for identifying a composition which stimulates or inhibits neuroblast proliferation or differentiation comprising:a) incubating components comprising a neuroblast and a composition to be tested for the ability to stimulate or inhibit proliferation or differentiation of the neuroblast,wherein the incubating is carried out under conditions and for a time sufficient to allow the composition to be tested to bind to the neuroblast and to allow the neuroblast to be cultured in the absence of oncogenic transformation and in a vessel in a serum-free basal media supplemented with about 1 ng/ml to 100 ng/ml of basic fibroblast growth factor,wherein a surface in the vessel allows attachment of the cells, andwherein the neuroblast proliferates for greater than seven days, and thereby can result in a long term continuously proliferating neuroblast culture; andb) detecting an effect of the composition of the proliferation or differentiation of the neuroblast, thereby identifying a composition that stimulates or inhibits neuroblast proliferation or differentiation.