Patent ID: 9163231
Filing Date: 2015-10-20
Classification: A61P,C12N

Abstract:
1. A set or library of recombinant expression vectors, wherein said set or library of recombinant expression vectors expresses a set or library of short hairpin ribonucleic acid (shRNA) molecules, each shRNA molecule comprises contiguously: (a) a variable region 17-30 nucleotides long, consisting of a sequence, wherein said sequence is either (i) substantially random; or (ii) comprises a first sub-region and a second sub-region, wherein said first sub-region is substantially random and said second sub-region has a first sequence common to said set or library of shRNA molecules; (b) a non self-complementary region 3-20 nucleotides long, consisting of a second sequence common to said library; and (c) a complementary region consisting of a sequence, wherein said sequence is the reverse complement of the variable region; wherein the substantially random sequence in the variable region has minimal sequence bias, wherein the variable and complementary regions form a double-stranded region and the non self-complementary region forms a loop that connects the two strands of the double-stranded region.