Patent ID: 6586214
Filing Date: 2003-07-01
Classification: C12N,C12P,C12Y

Abstract:
A process for increasing the metabolic flux through the pentose phosphate cycle in coryneform bacteria, said process comprising:(a) eliminating the intracellular activity of a phosphoglucose isomerase (pgi) enzyme comprising the amino acid sequence of SEQ ID NO:2 in said coryneform bacteria; and (b) fermenting the coryneform bacteria of step (a), wherein elimination is achieved by one or more methods of mutagenesis selected from the group consisting of deletion mutagenesis with deletion of two or more codons in the pgi gene; insertional mutagenesis due to homologous recombination; and transition or transversion mutagenesis with incorporation of a non-sense mutation in the pgi gene.