Patent ID: 8815606
Filing Date: 2014-08-26
Classification: G01N,Y10T

Abstract:
1. A thin-layer chromatography method using a single thin-layer chromatography plate and a single colorimetric reagent for detection and identification of both military explosives and peroxide explosives in samples using the single thin-layer chromatography plate and using the single colorimetric reagent, consisting of the steps of: providing a single reverse-phase thin-layer chromatography plate for detection and identification of both military explosives and peroxide explosives in samples; preparing said single reverse-phase thin-layer chromatography plate for detection and identification of both military explosives and peroxide explosives in sample by marking spots on which to deposit the samples; spotting one micro liter of a first standard onto one of said spots wherein said first standard is a standard for military explosives, spotting one micro liter of a second standard onto another of said spots wherein said second standard is a standard for peroxide explosives, and spotting samples onto other of said spots producing a spotted single reverse-phase thin-layer chromatography plate for detection and identification of both military explosives and peroxide explosives in sample; adding eluent to a developing chamber; adding said spotted single reverse-phase thin-layer chromatography plate to said developing chamber; removing said spotted single reverse-phase thin-layer chromatography plate from said developing chamber producing a developed single reverse-phase thin-layer chromatography plate; placing said developed single reverse-phase thin-layer chromatography plate in an ultraviolet light box; adding a single colorimetric visualization reagent to a dip tank, wherein said single colorimetric visualization reagent is diphenylbenzidine in EtOH; dipping said developed single reverse-phase thin-layer chromatography plate in said dip tank and remove said developed single reverse-phase thin-layer chromatography plate; and detecting the military explosives and detecting the peroxide explosives by viewing said developed single reverse-phase thin-layer chromatography plate.