Patent ID: 7935486
Filing Date: 2011-05-03
Classification: C12Q,G01N

Abstract:
1. A kit for detecting multiple pathogenic microorganisms comprising a hybridization module fixed with a fishing probe, a reporter probe labeled with a tag, a standard sample, a hybridization buffer solution, a washing solution, a denature solution, a neutralization solution, an antibody dilution solution, an enzyme linked anti-Tag antibody and a substrate solution of the enzyme, wherein the fishing probe is a single stranded polynucleotide designed to recognize one portion of a specific gene of the microorganism to be detected; the reporter probe is a double stranded polynucleotide designed to recognize the other portion of the specific gene which does not overlap with the portion recognized by the fishing probe; said denature solution consists essentially of 0.2 to 0.4N NaOH solution containing a 0.1 through 0.25% Tween 20; said neutralization solution contains 75 to 125 mM Tris-HCl (pH7.5), 125 to 175 mM NaCl and 1 to 2% Tween 20; and said antibody dilution solution contains 1% skim milk protein (w/v), 50 mM Tris-HCl (pH7.4) and 150 mM NaCl.