Patent ID: 7659095
Filing Date: 2010-02-09
Classification: C12N,C12Q

Abstract:
1. A method for identifying elite event RF-BN1 in a biological sample of a Brassica plant, Brassica plant material, or a product comprising a Brassica plant material, which method comprises determining whether a DNA fragment of between 100 and 350 base pairs from a nucleic acid present in said biological sample is amplified in a polymerase chain reaction with at least two primers, wherein a first primer comprises 19 consecutive nucleotides of nucleotides 46-881 of SEQ ID NO: 24 or nucleotides 1-595 of SEQ ID NO: 39, and a second primer comprises 19 consecutive nucleotides of the complement of nucleotides 882 to 1060 of SEQ ID NO: 24 or of nucleotides 596 to 694 of SEQ ID NO: 39, or wherein a second primer comprises 19 consecutive nucleotides of SEQ ID NO: 2, or the complement thereof, or wherein a first primer comprises 19 consecutive nucleotides of the complement of nucleotides 183-1457 of SEQ ID NO: 30 or nucleotides 42-1279 of SEQ ID NO: 40, and a second primer comprises 19 consecutive nucleotides of nucleotides 17-182 of SEQ ID NO: 30 or nucleotides 1 to 41 of SEQ ID NO: 40, or wherein a second primer comprises 19 consecutive nucleotides of SEQ ID NO: 2, or the complement thereof, and wherein the first and the second primer amplify the DNA fragment from a nucleic acid present in a biological sample of a wherein the first and the second primer do not amplify the DNA fragment from a nucleic acid present in a biological sample of a wherein if the DNA fragment is amplified, the biological sample comprises elite event RF-BN1.