Patent ID: 8551708
Filing Date: 2013-10-08
Classification: C12Q

Abstract:
1. A method for localized in situ detection of mRNA which codes for one or more mutations of the KRAS gene in a sample of cells that were formalin-fixed paraffin-embedded (FFPE) on a slide surface, comprising: (a) generating cDNA from mRNA in the sample using a primer; (b) digesting the mRNA hybridized to said cDNA; (c) contacting said sample with one or more padlock probes specific for mutations to the KRAS gene; (d) joining, directly or indirectly, the ends of said padlock probe(s) to form circularized padlock probe(s); (e) subjecting said circularized padlock probe(s) to rolling circle amplification (RCA) to generate rolling circle amplification products; and, (f) detecting the rolling circle amplification product(s), wherein the padlock probe comprises: