Patent ID: 6054632
Filing Date: 2000-04-25
Classification: A01K,C07K,C12N

Abstract:
A method of making an antibody, comprising:constructing a first transgenic mouse whose somatic and germ cells comprise a polynucleotide sequence encoding one human Duffy protein polymorph of either Fy.sup.a or Fy.sup.b and whose somatic cells express either the Fy.sup.a or Fy.sup.b polymorph encoded by the polynucleotide;constructing a second transgenic mouse whose somatic and germ cells comprise a polynucleotide sequence encoding the human Duffy protein polymorph Fy.sup.a or Fy.sup.b which is not contained in said first transgenic mouse and whose somatic cells express either the Fy.sup.a or Fy.sup.b human Duffy protein polymorph not expressed in said first transgenic mouse, wherein said second transgenic mouse is syngeneic to said first transgenic mouse;immunizing said first transgenic mouse with cells from said second transgenic mouse to induce an immune response in said first transgenic mouse, wherein a lymphoid cell of said first transgenic mouse produce an antibody specific for an epitope of the human Duffy protein polymorph expressed by the somatic cells of said second transgenic mouse; andisolating the antibody.