Patent ID: 6958225
Filing Date: 2005-10-25
Classification: C12N,C12Q,Y10T

Abstract:
1. A method for analyzing a nucleic acid sample comprising: fragmenting the nucleic acid sample using a first and a second restriction enzyme to produce fragments; ligating a first and a second adaptor to the fragments to produce adaptor-ligated fragments, wherein the first adaptor is blocked from ligation to the fragments at the 3′ end of one strand of the first adaptor, and the second adaptor is blocked from ligation to the fragments at the 5′ end of one strand of the second adaptor and wherein the first adaptor ligates to the fragments cut by the first restriction enzyme and the second adaptor ligates to the fragments cut by the second restriction enzyme; amplifying the adaptor-ligated fragments to produce amplified fragments, wherein the adaptor-ligated fragments that contain both the first adaptor and the second adaptor are enriched in the amplified fragments relative to the adaptor-ligated fragments that contain the first adaptor and do not contain the second adaptor, and to the adaptor-ligated fragments that contain the second adaptor and do not contain the first adaptor; providing a nucleic acid array consisting essentially of probes designed to detect the bases present at a plurality of polymorphisms predicted to be present in the amplified fragments; hybridizing the amplified fragments to the array; and analyzing a hybridization pattern resulting from the hybridization.