Patent ID: 6114600
Filing Date: 2000-09-05
Classification: C12N

Abstract:
A method of resolving multiple copies of a construct integrated at a locus in a plant cell to a single copy of said construct at said locus, the method comprising:introducing into the cell a construct comprising an exogenous polynucleotide sequence flank by a first pair of recombination sites oriented such that contact with a recombinase would not result in deletion of the polynucleotide sequence from the construct to obtain a cell which comprises multiple copies of the construct integrated at a locus of a cell chromosome; andcontacting the locus with a recombinase that catalyzes recombination among the recombination sites of the multiple copies at said locus;wherein the recombination results in resolution of the multiple copies of the introduced construct into a single copy of the construct integrated at said locus.