Patent ID: 8034994
Filing Date: 2011-10-11
Classification: C12N

Abstract:
1. A method for starchy-endosperm- and/or germinating embryo-specific expression of a nucleic acid sequence in a monocotyledonous plant, comprising: (a) constructing an expression cassette by operably linking at least one transcription regulating nucleotide sequence to at least one nucleic acid sequence which is heterologous to said at least one transcription regulating nucleotide sequence, (b) transforming said expression cassette into a monocotyledonous plant, and (c) selecting a transgenic plant demonstrating starchy-endosperm- and/or germinating embryo-specific expression of said at least one nucleic acid sequence, wherein the at least one transcription regulating nucleotide sequence comprises a nucleotide sequence selected from the group consisting of: (i) the sequence of SEQ ID NO: 4; (ii) a fragment of the sequence of SEQ ID NO: 4, wherein the fragment directs starchy-endosperm- and/or germinating embryo-specific expression of said at least one nucleic acid sequence; (iii) a nucleotide sequence having at least 95% sequence identity to the sequence SEQ ID NO: 4, wherein the nucleotide sequence comprises the DNA motifs provided in Table 9 and directs starchy-endosperm- and/or germinating embryo-specific expression of said at least one nucleic acid sequence; (iv) a nucleotide sequence having at least 95% sequence identity to the sequence of SEQ ID NO: 4, wherein the nucleotide sequence further comprises the sequences of SEQ ID NO: 1 and SEQ ID NO: 2 and directs starchy-endosperm- and/or germinating embryo-specific expression of said at least one nucleic acid sequence; and (v) a nucleotide sequence which is complement or reverse complement of any of the nucleotide sequence of (i) to (iv).