Patent ID: 7504238
Filing Date: 2009-03-17
Classification: C12N,C12Q

Abstract:
1. A process for directionally ligating a double-stranded nucleic acid to a first adaptor sequence, the process comprising: a. forming an amplification product from the double-stranded nucleic acid using a mixture comprising b. treating the amplification product with the exonuclease to form a digested amplicon having a first overhang sequence at a first termini and a second overhang sequence at a second termini, wherein the first and second termini of said digested amplicon terminate at the sites of incorporation of said modified dNTPs, and wherein the first overhang sequence at the first termini is complementary to the first ligation site sequence of the first adaptor sequence and the second overhang sequence at the second termini is complementary to the second ligation site sequence of the second adapter; and c. ligating the first overhang sequence of the digested amplicon to the first ligation site sequence of the first adaptor sequence.