Patent ID: 8501480
Filing Date: 2013-08-06
Classification: B07C,C11B,G01N

Abstract:
1. A method of accumulating a quantity of monocot seeds having a desired fatty acid characteristic, the method comprising: (a) removing a sample from each seed in a population of monocot seeds while maintaining the germination viability of the seeds; (b) contacting each sample with a solvent to form a mixture comprising fatty acid methyl esters; (c) analyzing the mixture of fatty acid methyl esters from each sample to determine the fatty acid profile of the corresponding seed, wherein the fatty acid profile of the corresponding seed is determined in less than 3 minutes from the time in which an individual tissue sample is contacted with solvent; (d) selecting seeds having at least one desired fatty acid characteristic based on the analysis of the samples removed from the seeds; (e) cultivating plants form the selected seeds; (f) recovering seeds from the cultivated plants, wherein the recovered seeds are a subsequent generation of the selected seeds; and Repeating steps (a) through (f) for one or more generations of the recovered seeds to thereby accumulate the quantity of seeds having the desired fatty acid characteristic.