Patent ID: 8609340
Filing Date: 2013-12-17
Classification: C12Q

Abstract:
1. A method for controlled amplification of at least a first and a second target nucleic acid from a plurality of biological samples, comprising: a) adding an internal control nucleic acid to each of the plurality of biological samples in a number of reaction vessels corresponding to the number of biological samples, wherein the internal control nucleic acid is identical for each of the plurality of biological samples, and wherein the sequence of the internal control nucleic acid is different from the sequences of the target nucleic acids; and b) purifying target nucleic acids from the plurality of biological samples and the internal control nucleic acid simultaneously in the reaction vessels; c) adding amplification reagents to the reaction vessels, d) incubating the reaction vessels simultaneously under identical conditions sufficient for amplification to occur; e) detecting and measuring signals generated by the amplification;