Patent ID: 7983849
Filing Date: 2011-07-19
Classification: C12N,G16B

Abstract:
1. A method to determine the mutational load of a gene bank obtained by random mutagenesis of a gene of interest, comprising the following steps: a) obtaining a chart linking the mutational load (ML) of the gene bank obtained by random mutagenesis of a model gene with the fraction of mutated model genes observed in said bank; b) performing, in parallel, the random mutagenesis of the model gene used for the preparation of the chart for step (a) and the gene of interest to obtain the corresponding mutated gene banks; c) determining the mutational load (ML) of the gene bank obtained using the model gene in step (b) on the basis of the chart plotted in step (a); d) applying a correction factor (CF) to the mutational load (ML) of the mutated model gene bank determined in step (c) to determine the mutational load (ML) in the gene bank of the mutated gene of interest from step (b), wherein the correction factor (CF) comprises at least two intrinsic parameters of a characteristic of a gene and at least one experimental parameter.