Patent ID: 8912138
Filing Date: 2014-12-16
Classification: A61K,A61P,C07K,C12N,C12Y

Abstract:
1. A method of modifying an endogenous gene in a region of interest in a cell, the method comprising: introducing into the cell a polynucleotide encoding a fusion protein, the fusion protein comprising (a) an isolated, non-naturally occurring DNA-binding polypeptide comprising: two or more TALE-repeat units, the TALE repeat units comprising a repeat variable di-residue (RVD); an N-cap polypeptide, wherein the N-cap comprises residues N+1 to N+121 of a TALE protein; and a C-cap polypeptide, wherein the C-cap polypeptide comprises a fragment of a full length C-terminus region of a TALE protein, the C-cap comprising residues C-1 to C-20 of the TALE protein, and (b) a functional domain, wherein the fusion protein binds to a target site in the endogenous gene and further wherein the endogenous gene is modified.