Patent ID: 7067266
Filing Date: 2006-06-27
Classification: C12Q,G01N

Abstract:
1. A method of screening for an immunosuppressant, comprising: a) combining a candidate bioactive agent, an HCK protein, and an HCK substrate protein, wherein said HCK protein comprises an amino acid sequence having at least about 95% identity to the amino acid sequence set forth in SEQ ID NO:2; b) detecting an inhibition of phosphorylation of said HCK substrate protein in the presence of said candidate bioactive agent; c) contacting said candidate bioactive agent to a lymphocyte; d) inducing activation of said lymphocyte; and e) determining the activation of said lymphocyte in the presence of said candidate bioactive agent; wherein said HCK protein will phosphorylate said HCK substrate protein in the absence of said candidate bioactive agent, and wherein a decrease in the activation of said lymphocyte in the presence of said candidate bioactive agent indicates that said candidate bioactive agent is an immunosuppressant.