Patent ID: 7582455
Filing Date: 2009-09-01
Classification: C12N,C12P

Abstract:
1. A method for producing monatin, or a salt thereof, comprising reacting D-tryptophan and one or more D-aminotransferases chosen from a Bacillus halodurans D-aminotransferase, a hybrid D-aminotransferase having the sequence of SEQ ID NO:99, a Geobacillus stearothermophilus D-aminotransferase, a Bacillus lichenformis D-aminotransferase, a D-aminotransferase from a Bacillus sphaericus deposited with American Type Culture Collection (ATCC) and designated ATCC 4978, and a D-aminotransferase from a Bacillus sphaericus deposited with ATCC and designated ATCC 7063, wherein D-tryptophan is converted to indole-3-pyruvate, indole-3-pyruvate is converted to 2-hydroxy-2-(indoly-3-ylmethyl)-4-keto glutaric acid (MP), and MP is converted to monatin.