Patent ID: 7105318
Filing Date: 2006-09-12
Classification: C12Q

Abstract:
1. A method for the detection of several nucleic acids comprising the steps: (a) simultaneously producing in a multiplex reaction a plurality of amplificates of sections of the nucleic acids with the aid of pairs of two primers, of which in each case one can bind to a binding sequence (A′), which is essentially complementary to a sequence A of a strand of the nucleic acid, and of which the other can bind to a binding sequence C which is located in the 3′ direction from A and does not overlap A, wherein the primers are selected such that the amplificates that are formed do not differ by more than 20% in length and are not longer than 100 nucleotides; (b) contacting the respective amplifcates with a probe that in each case has a binding sequence D or D′, which can bind either to the sequence B located between the sequences A and C or to the complement thereof; and (c) detecting the formation of a hybrid of the amplificate and a probe; wherein the sequence located between the binding sequences A and C contains no nucleotides or less than 3 nucleotides that do not belong to the sequence section E formed from the binding sequence D of the probe and the sequence of the amplificate bound thereto and the amplificates are shorter than 100 nucleotides.