Patent ID: 6902886
Filing Date: 2005-06-07
Classification: C07H,C07K,C12Q

Abstract:
1. A method of determining the presence of a nuclear localization signal in a protein of interest, the method comprising: selecting a host cell for use in the method, wherein the host cell contains a nucleus having nucleic acid encoding a reporter gene therein and wherein the host cell has a first level of expression of the reporter gene; identifying a DNA binding domain and an activation domain for the reporter gene; constructing a chimeric nucleic acid encoding a fusion protein comprising the DNA binding domain, the activation domain, and a protein of interest, wherein elements of the fusion protein other than the protein of interest have no nuclear localization signals; introducing the chimeric nucleic acid into the host cell; and determining a second level of expression of the reporter gene to determine the presence of a nuclear localization signal in the protein of interest.