Patent ID: 7026114
Filing Date: 2006-04-11
Classification: C07H,C12Q,G01N

Abstract:
1. A method of monitoring polymer array synthesis on a solid substrate comprising: (i) synthesizing a preselected array of diverse biological polymers connected to a solid substrate, whereby the diverse biological polymers occupy different regions of the substrate and are spatially defined on the solid substrate on which the preselected array is synthesized, and wherein the diverse biological polymers comprise nucleotides, nucleosides, phosphoramidites, carbohydrates or natural or synthetic amino acids; (ii) cleaving the diverse biological polymers from the solid substrate thereby creating a mixture of diverse unbound biological polymers; and (iii) predicting a quantity of diverse biological polymers formed and comparing a measurement of quantity of diverse cleaved biological polymers from the array with the predicted quantity of diverse biological polymers formed as an indicator of the efficiency of the synthesis procedure, thereby determining the efficiency of the synthesis procedure.