Patent ID: 6777229
Filing Date: 2004-08-17
Classification: C12N,C12P

Abstract:
An isolated and purified plasmid capable of autonomous replication in bacteria of the genus Corynebacterium, said plasmid comprising:i) at least one region that encodes a protein involved in a biosynthetic pathway selected from the group consisting of L-lysine and pantothenic acid; ii) at least one DNA replication region obtained from one of the plasmids pTET3 or pCRY4, and iii) at least one region that encodes a protein for antibiotic resistance comprising a gene selected from the group consisting of a gene encoding a protein conferring tetracycline resistance, a gene encoding a protein conferring streptomycin and spectinomycin resistance, and a gene conferring sulfamethoxazole resistance, wherein said genes are obtained from the antibiotic resistance region of plasmid pTET3, as set forth in FIG. 5.