Patent ID: 7776607
Filing Date: 2010-08-17
Classification: A01K,A61K,C07K

Abstract:
1. A method of determining a human subject's susceptibility to developing colon or head and neck cancer by determining a genotype of a mammalian 15 kDa selenoprotein gene encoding a polypeptide comprising the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 4 in a tissue sample from the subject, comprising: isolating DNA or mRNA from the sample; optionally synthesizing cDNA from the RNA; amplifying the DNA, cDNA, or mRNA in the 3′-untranslated region of the gene containing polymorphism at nucleotide positions corresponding to those nucleotides occurring at positions 811 and 1125 of SEQ ID NO: 2; digesting the amplified DNA, cDNA or mRNA with restriction enzyme(s) which can distinguish the polymorphism by a differential restriction fragment length; detecting the polymorphism by the presence of the differential fragment length; and determining that the subject is susceptible to developing colon or head and neck cancer if polymorphism is present at nucleotide positions 811 and/or 1125 of the gene.