Patent ID: 9134318
Filing Date: 2015-09-15
Classification: G01N

Abstract:
1. A method for determining whether a subject is afflicted with diabetes mellitus comprising: obtaining a blood or plasma sample from said subject comprising at least one carbonylated protein selected from Table 15, wherein the carbonylated protein comprises a plurality of carbonylated sites; isolating the carbonylated protein from the other oxidized proteins in the sample via an affinity purification technique, wherein the step of isolating comprises: digesting the isolated carbonylated protein into a plurality of different carbonylated peptides; determining and quantitatively assessing by a liquid chromatography-mass spectrometry technique a degree of oxidation at each of a plurality of carbonylated sites found in one or more of the carbonylated peptides obtained from the following carbonylated proteins from as disclosed in Table 15: hemoglobin alpha 2 chain; major beta-hemoglobin; fibrinogen alpha chain; fatty acid binding protein, immunoglobulin heavy chain, and complement component 3; comparing the degree of oxidation at each of the plurality of carbonylated sites to a degree of oxidation at each of the same sites found in one or more reference peptides; and detecting a difference in the degree of oxidation at the plurality of the carbonylated sites between the one or more carbonylated peptides and the reference peptides, wherein a greater than 1.5 fold increase in the degree of oxidation at the plurality of the carbonylated sites is indicative of the subject being afflicted with diabetes mellitus.