Patent ID: 8222005
Filing Date: 2012-07-17
Classification: C12N,C12Q

Abstract:
1. A method for preparing at least one ditag comprising the steps of: (i) producing at least one full-length cDNA transcript, said transcript having a 5′ terminus including the starting point of the full-length cDNA transcript and a 3′ terminus including the ending point of the full-length cDNA transcript; (ii) cleaving the full-length cDNA transcript at its 5′ terminus to extract a 5′ tag 8-20 base pairs (bp) in length, said 5′ tag having a 5′ end and a 3′ end and including the starting point of the full-length cDNA transcript, and cleaving the full-length cDNA transcript at its 3′ terminus to extract a 3′ tag 8-20 bp in length, said 3′ tag having a 5′ end and a 3′ end and including the ending point of the full-length cDNA transcript; and (iii) generating at least one ditag by ligating the 3′ end of the 5′ tag to the 5′ end of the 3′ tag.