Patent ID: 8609342
Filing Date: 2013-12-17
Classification: C12Q

Abstract:
1. A cell comprising (i) a polynucleotide sequence encoding luciferase, wherein said polynucleotide sequence is functionally linked to a first transcriptional regulatory sequence having a polynucleotide sequence recognizing a sex-hormone receptor, (ii) a polynucleotide sequence encoding GFP, wherein said polynucleotide sequence encoding GFP is functionally linked to a second transcriptional regulatory sequence, wherein the second transcriptional regulatory sequence is different from said first transcriptional regulatory sequence and does not have a polynucleotide sequence recognizing a sex-hormone receptor, and (iii) a polynucleotide sequence encoding the sex-hormone receptor, wherein the cell is a CHO cell, wherein the polynucleotide of (iii) is in a sex-hormone receptor expression vector comprising a drug resistance gene and a polynucleotide encoding the sex-hormone receptor, wherein the polynucleotide of (ii) is in a GFP expression vector comprising a drug resistance gene and a polynucleotide encoding GFP, and the polynucleotide of (i) is in a luciferase expression vector comprising a drug resistance gene and a polynucleotide encoding luciferase, and wherein the first transcriptional regulatory sequence has a polynucleotide sequence encoding a heat shock promoter, and the second transcriptional regulatory sequence has a polynucleotide sequence encoding a CMV promoter.