Patent ID: 9103790
Filing Date: 2015-08-11
Classification: A01K,C07K,C12Q,G01N

Abstract:
1. A linker for a unimolecular fluorescence resonance energy transfer (FRET) biosensor based on a principle of fluorescence resonance energy transfer, the linker comprising: a polypeptide comprising 84 to 244 amino acids residues, wherein the polypeptide consists of (i) glycine, (ii) alanine, and (iii) serine, threonine or both thereof, wherein 35% to 65% of a total number of the amino acid residues are glycine, 10% to 40% of the total number of the amino acid residues are alanine, and 10% to 40% of the total number the amino acid residues are serine, threonine or both thereof, and wherein the polypeptide has repeats of an amino acid sequence of SAGG, and wherein the number of the repeats of the amino acid sequence of SAGG is 19 to 54, and wherein the polypeptide is selected from a sequence consisting of SEQ ID NO: 15, SEQ ID NO: 18, SEQ ID NO: 23 and SEQ ID NO: 26.