Patent ID: 7462449
Filing Date: 2008-12-09
Classification: B01L,C12Q,F04B,F15C,F16K,G01N

Abstract:
1. A method of deriving nucleotide sequence information from polynucleotide molecules, the method comprising the steps of: immobilizing a plurality of target nucleic acid template molecules on a 2-dimensional substrate, said molecules tagged with a first optically-detectable label attached to each of said molecules; exposing said molecules to a polymerase and at least one nucleotide comprising a second optically-detectable label under conditions that allow said nucleotide to be incorporated in a primer hybridized to said template molecule if said nucleotide is complementary to a nucleotide in said template immediately 3′ of said primer; rinsing said substrate to remove unincorporated nucleotides; positionally locating said second optically-detectable labels appearing in said substrate; associating each of said positions with a specific polynucleotide in said substrate; inactivating said second optically-detectable label; repeating said exposing, rinsing, and locating steps at least once; and recording a sequence of nucleotides incorporated in said primer at each of said positions in order to determine a sequence of nucleotides in said template, wherein said substrate is coated with a polyelectrolyte multilayer.