Patent ID: 9101902
Filing Date: 2015-08-11
Classification: B01J

Abstract:
1. A method for preparing a biochip by gelation of a sol composition, the method comprising the steps of (a) and (b) in sequential order: (a) dispensing onto a substrate a sol composition consisting of SolB1, SolB2 and SolB3 in a spot form and dispensing solution I comprising SolBH solution of an acid selected from the group consisting of HCl, H (b) dispensing solution II, comprising buffer, a biological material which interacts with target biological material, and distilled water onto the substrate onto which the solution I has been dispensed, and then gelling the dispensed solutions, wherein the method does not have a pretreatment process, which is one or more selected from the group consisting of (i) mixing the sol composition, solution I and solution II; (ii) vortexing the mixed solution of (i); and (iii) stabilizing the mixed solution of (i) or (ii); wherein (i) said SolB1 is at least one first silicate monomer selected from the group consisting of methyltriethoxysilane (MTES), ethyltriethoxysilane (ETrEOS), sodium silicate, tetramethyl orthosilicate (TMOS), tetraethyl orthosilicate (TEOS) and tetramethoxysilicate (TMS); wherein (ii) said SolB2 is at least one compound selected from the group consisting of 3-aminotrimethoxysilane (3-ATMS), diglycerylsilane (DGS), methyltrimethoxysilicate (MTMS), polyglycerylsilicate (PGS), polyvinylacetate, polyvinylpyrrolidone, glyceryl methacrylate, hydroxyethyl acrylate, N,N-disuccinimidyl carbonate (DSC), 1,3,5-trimethylbenzene, cetyltrimethylammonium chloride, cetyltrimethylammonium bromide, 3-(triethoxysily)propyl succinic anhydride, N-(3-triethoxysilypropyl)-4-hydroxy butylamide (SIT8189.5), N-(triethoxysilylpropyl)gluconamide (SIT8189.0), pluronic L121 and tetramethyl ammonium hydroxide; and wherein (iii) said SolB3 is at least one additive selected from the group consisting of aminopropyltriethoxysilane (APTES), 3-glycidoxypropyltrimethoxysilane (GPTMOS), N-triethoxysilylpropyl-O-polyethylene oxide urethane (PEOU), glycerol, PEG200, PEG400, PEG600, PEG1350 and PEG8000.