Patent ID: 7468249
Filing Date: 2008-12-23
Classification: C12Q

Abstract:
1. A method for detecting any one of multiple chromosomal disorders in a single assay, which method comprises the steps of: a. making a polymerase chain reaction (PCR) mixture by mixing in a vessel components comprising: b. conducting a PCR for between about 5 and about 60 temperature cycles to create amplified PCR products; c. purifying said products of step (b) and obtaining single-stranded DNA having the detectable labels, d. contacting a microarray with products of step (c), which microarray has a plurality of spots that each contain DNA oligonucleotide probes having nucleotide sequences complementary to a nucleotide sequence of one of said strands of each of said targeted segments; e. hybridizing said DNA oligonucleotide probes and said PCR-amplified label-containing single-stranded products; f. detecting the presence and relative quantity of the PCR-amplified products hybridized to the microarray by imaging the microarray; and g. diagnosing whether or not a chromosomal disorder exists with respect to one or more of said selected different chromosomes by comparing said imaging of the relevant spots on said microarray for each said targeted segment of a selected chromosome to the imaging of spots relevant to said control gene to determine a ratio of such intensities for each of a plurality of said targeted segments, termed an I-ratio, then dividing each said I-ratio by an N-ratio obtained from similar testing of multiple samples of genomic DNA of the same gender known to be normal to obtain a C-factor for each, averaging all said C-factors to obtain an average C-factor, then adjusting each said I-ratio by said average C-factor, and then comparing each said adjusted I-ratio to the respective N-ratio to determine if a disorder exists.