Patent ID: 8055451
Filing Date: 2011-11-08
Classification: G16B

Abstract:
1. A method for estimating a melting temperature, T m (X 2+ ), for a polynucleotide having a known G-C content value, f GC , at a desired divalent ion concentration, [X 2+ ], and an optionally present monovalent cation concentration [Mon + ], the method carried out by a computer system executing instructions contained in at least one memory, the method comprising: (a) the computer system receiving a reference melting temperature, T (b) the computer system generating the melting temperature of the polypeptide at the desired monovalent and divalent cation concentrations by modifying said reference melting temperature, or reciprocal of said melting temperature, by one or more terms which are a function of fGC f wherein the reciprocal of the reference melting temperature is modified by adding a term comprising: wherein each of the coefficients a, b, c, and d is optimized for predicting polynucleotide melting temperatures.