Patent ID: 6143504
Filing Date: 2000-11-07
Classification: C12Q

Abstract:
A method for determining the methylation state of an FMR1 gene promoter of a male subject comprising the steps of:(a) denaturing a DNA sample from said subject;(b) subjecting the denatured DNA to bisulfite modification;(c) amplifying said DNA using primers pairs having the sequencesACCGATTCCCAACAACGCGCATA (SEQ ID NO:12) andTTTCGTTATCGTCGTCGTTCGC (SEQ ID NO:13), andACACACATACACACACTCCCAAA (SEQ ID NO:14) andTTGAAATGGAGTTGAGTGTTTGAT (SEQ ID NO:15); and(d) detecting amplification products from step (c).