Patent ID: 6806056
Filing Date: 2004-10-19
Classification: C12Q,G01N

Abstract:
A method for screening a substance for the ability to modulate tyrosine kinase activity comprising the steps:(a) exposing a portion of a biological sample to said substance; (b) determining the level of tyrosine kinase activity in said exposed portion and in an unexposed portion by: (1) contacting said portions individually with a solid phase coated with a first anti-phosphotyrosine antibody so that phosphorylated tyrosine-containing molecules in the biological sample are bound by the antibody to the solid phase; (2) contacting the phosphorylated tyrosine-containing molecules that are bound by the first anti-phosphotyrosine antibody to the solid phase with a second labeled anti-phosphotyrosine antibody; and (3) measuring the amount of labeled anti-phosphotyrosine antibody bound to the solid phase, wherein the amount of labeled anti-phosphotyrosine antibody indicates the level of tyrosine kinase activity in the portions; and (c) comparing the level of tyrosine kinase activity in said exposed portion with the level of tyrosine kinase activity in the unexposed portion, wherein a difference between the levels indicates that the substance has the ability to modulate tyrosine kinase activity.