Patent ID: 8828666
Filing Date: 2014-09-09
Classification: A61P,C07K,G01N

Abstract:
1. A method of detecting the binding of an antibody to both an antigen or antigen epitope and an Fc receptor or a binding fragment thereof simultaneously, comprising: mixing the antibody with the antigen or antigen epitope labeled with one member of a set of donor and acceptor capable of fluorescent resonance energy transfer and the Fc receptor or binding fragment thereof labeled with the other member of the set of donor and acceptor; irradiating the resultant mixture with light having a wavelength capable of exciting the donor; measuring the fluorescence level of said mixture; and indicating the binding of the antibody to both the antigen or antigen epitope and the Fc receptor or binding fragment thereof simultaneously based on said fluorescence level, wherein the Fc receptor is selected from the group consisting of FcγR, FcεR, and FcαR.