Patent ID: 7527929
Filing Date: 2009-05-05
Classification: C12N,C12Q

Abstract:
1. A method of selectively isolating a target species of nucleic acid molecule present in a mixture, comprising: a) combining the mixture with solid phase carriers having a surface comprising a first functional group which binds nucleic acids and a second functional group which selectively binds the target species of nucleic acid, thereby producing a first combination; b) maintaining the first combination under conditions appropriate for binding of the nucleic acids to the first functional group; c) separating the solid phase carriers from the first combination; d) combining the solid phase carriers with at least one agent that removes the nucleic acid from the first functional group of the solid phase carriers and binds the target species of nucleic acid to the second functional group of the solid phase carriers, thereby producing a second combination; and e) separating the solid phase carriers from the second combination, thereby isolating the target species of nucleic acid present in the mixture comprising nucleic acids.