Patent ID: 8673568
Filing Date: 2014-03-18
Classification: C12Q,Y02A

Abstract:
1. An in vitro method for molecular typing and/or subtyping of a bacterium of the Salmonella genus, using a sample, said method being characterized in that it comprises at least the following steps: (a) amplifying a nucleic acid fragment from a bacterium of the a set of primers “A”, capable of amplifying a nucleic acid fragment comprising the CRISPR1 locus, comprising at least one forward primer A 1 constituted of an oligonucleotide sequence which exhibits at least 70% identity with, or which is identical to, a fragment of the genomic sequence of a bacterium of the a set of primers “B”, capable of amplifying a nucleic acid fragment comprising the CRISPR2 locus, comprising at least one forward primer B1 constituted of an oligonucleotide sequence which exhibits at least 70% identity with, or which is identical to, a fragment of the genomic sequence of a bacterium of the a set of primers “C”, capable of amplifying a nucleic acid fragment comprising the CRISPR1 locus and the CRISPR2 locus, comprising at least one forward primer A1as defined above and at least one reverse primer B2 as defined above; (b) determining the number and the nucleotide sequence of the variable sequences of the CRISPR1 locus and/or of the CRISPR2 locus which is (are) amplified in step (a); and (c) comparing said variable-sequence composition of the CRISPR1 and/or CRISPR2 loci with a reference base which provides the variable-sequence composition of the CRISPR1 and CRISPR2 loci of types and subtypes of bacteria of the