Patent ID: 6265217
Filing Date: 2001-07-24
Classification: A01H,C12N

Abstract:
A method for producing microbulbs of Garlic in vitro by culturing tissues, comprising the steps of:a) isolating and excising virus-free tissues having a length of between 0.2 and 0.3 mm;b) inoculating the excised tissues from meristem tissue of Garlic onto a primary solid media containing 6.5 g/l of agar;c) culturing the tissues inoculated onto the primary solid media containing 6.5 g/l of agar under a cyclable sixteen hour light period and eight hour darkness period for four weeks;d) propagating plantlets regenerated from the cultured tissues at propagating media for four weeks;e) transferring the propagated plantlets to a liquid media, adding 90 g/l of sucrose and plant growth regulators to the liquid media, and culturing primarily;f) transferring the primarily cultured tissues to the liquid media having the same composition as the media used in step e, and adding 140 g/l of sucrose and plant growth regulators to the liquid media;g) secondarily culturing the tissues of step e in the room under 20 lux of illumination for six weeks; andh) harvesting the microbulbs from the virus-free garlic plants in vitro in which steps f and g are carried out in Murashige-Skoog liquid media having a reduced quantity of NH.sub.4 NO.sub.3, and culturing under 20 lux of illumination.