Patent ID: 7932027
Filing Date: 2011-04-26
Classification: C12Q

Abstract:
1. A method for determining a DNA methylation pattern, comprising: a) obtaining a solution comprising a sized-biased amplified mixture of genomic DNA restriction fragments, wherein the generation of said mixture comprises an enrichment step using proteins that bind methylation-specifically to the DNA, and wherein the composition of the restriction fragment mixture depends on the methylation pattern of the genomic DNA; b) coupling the amplified fragments with a detectable label to provide a labeled fragment amplificate; c) contacting the labeled fragment amplificate with at least one DNA microarray having a plurality of different nucleic acids assigned to different respective array locations, wherein hybridization of amplificate fragments with the corresponding assigned nucleic acids takes place under definable stringency, and wherein the assigned nucleic acids are specific for genomic fragments obtainable by cutting with the restriction enzymes used in a); and d) detecting the label of the hybridized fragment amplificate using a suitable detection method, wherein determination of a hybridization pattern of the assigned array locations is afforded to further afford determination of the genomic DNA methylation pattern.