Patent ID: 7704692
Filing Date: 2010-04-27
Classification: C12Q

Abstract:
1. A process for detecting a toxic compound in a test material, wherein said toxic compound is selected from the group consisting of 2,5-dichlorophenol, 4-nitroquinolin-N-oxide, p-nonylphenol, 2,4,5-trichlorophenol, Trp-P-2(acetate), malathon, manganese ethylenebis (dithiocarbamate), nickel (II) chloride, potassium bichromate, phenol and dimethylsulfoxide, wherein said process comprises: (1) contacting the test material to a cell that is transformed with a polynucleotide or with a vector comprising said polynucleotide, wherein said polynucleotide comprises a polynucleotide sequence operably linked to a polynucleotide encoding a marker protein, and wherein said polynucleotide sequence comprises SEQ ID NO: 3; and (2) detecting said toxic compound via an expression of mRNA encoding a marker protein, wherein the mRNA expression is a result of said toxic compound activating expression of the gene within SEQ ID NO: 3.