Patent ID: 6287441
Filing Date: 2001-09-11
Classification: C12Q

Abstract:
A method for determining a combination of sets of electrophoresis parameters which are useful in an SSCP analysis for maximizing the number of different mutations in a nucleic acid sample which can be detected using a combination of said sets of electrophoresis parameters wherein said SSCP analysis comprises running two or more gels using a different set of electrophoresis parameters with each gel wherein said method comprises:a) performing SSCP analysis on said nucleic acid sample using multiple sets of electrophoresis parameters,b) calculating SSCP efficiencies,c) calculating a correlation coefficient of efficiencies, andd) determining which of the multiple sets of electrorhoresis parameters yield the highest average efficiencies and the lowest correlation coefficients of the sets,wherein the most useful combinations of sets of electrophoresis parameters of the multiple sets are those having the highest average SSCP efficiencies and the lowest or most negative correlation coefficients.