Patent ID: 8465947
Filing Date: 2013-06-18
Classification: A61P,C07K,C12N,C12R

Abstract:
1. A method for producing a host cell suitable for expressing a type B lantibiotic peptide deoxyactagardine, actagardine or a variant thereof, the method comprising the step of transforming a cell by introducing: a LanA gene, said gene encoding a LanA polypeptide comprising actagardine, actagardine B, actagardine B variant Ll, ala(0)-actagardine, ala(0)-actagardine B, ala(0)-actagardine B variant Ll, the polypeptide having the structure wherein -X1-X2- is -Leu-Val, -Val-Ile- or -Leu-Ile-; Y is —S—or —S(O)—; and Z is either H 2 N— or an amino acid, or an amino acid variant of any one of the same wherein: 1 to 5 amino acids in the LanA polypeptide sequence at positions 0 (Z), 2, 3, 4, 5, 8, 10, 11, 13 and 18 is/are substituted by another amino acid, and optionally introducing the LanA gene together with one or more associated cluster genes suitable for conversion of a precursor peptide into said peptide, thereby generating a host cell comprising a polynucleotide sequence encoding said peptide and suitable for expressing same.