Patent ID: 8185318
Filing Date: 2012-05-22
Classification: G01N

Abstract:
1. A method of determining a dose-response curve of one or more assays, where said one or more assays are sensitive to the effects of a perturbing variable, comprising the steps of: A) characterizing a dependence of said dose-response curve of one or more assays on said perturbing variable by the steps of, i) measuring assay signals from a suitable set of known standards for said one or more assays at different values of said perturbing variable, and also measuring one or more other signals dependent on said perturbing variable, and whereby said one or more other signals dependent on said perturbing variable are measured for each of said different values of said perturbing variable; ii) fitting said measured assay signals to a suitable functional form to produce a dose-response curve for each of said one or more assays at each of said different values of said perturbing variable and determining assay parameters from said fitting; and iii) establishing a mathematical relationship between each assay parameter of said one or more assays and each of said one or more other signals dependent on said perturbing variable; B) when performing one or more assays of unknown samples, determining a dose-response curve that compensates for the effects of changes in the value of said perturbing variable by the steps of: iv) re-measuring said one or more other signals dependent on said perturbing variable; v) obtaining a value of each assay parameter of said one or more assays by substituting one of said one or more re-measured other signals dependent on said perturbing variable into said mathematical relationship between said each assay parameter and said one of said one or more other signals dependent on said perturbing variable, and repeating said substitution for each of said one or more other signals dependent on said perturbing variable to obtain one or more values of said each assay parameter; and vi) producing a compensated dose-response curve for each of said one or more assays of said unknown samples by averaging said one or more values of each assay parameter of said one or more assays.