Patent ID: 7504209
Filing Date: 2009-03-17
Classification: B01J,C12Q,C40B

Abstract:
1. A method for integrated ribonucleic acid integrity assessment and analysis comprising the steps of: (a) providing a support having thereon a set of immobilized detector probes and a set of at least two control probes, wherein (b) contacting said support with analyte ribonucleic acids derived from a sample, under conditions allowing hybridization of complementary analyte ribonucleic acids and immobilized probes to form analyte ribonucleic acid/probe complexes; wherein said hybridization generates a detectable signal; (c) detecting signals generated by said complexes; (d) determining the ratio of signals generated by the 28S rRNA/control probe and 18S rRNA/control probe or the 23S rRNA/control probe and 16S rRNA/control probe or functionally equivalent rRNA/control probe complexes; (e) assessing the integrity of said sample; and (f) evaluating microarray analysis results in view of said integrity assessment.