Patent ID: 6780841
Filing Date: 2004-08-24
Classification: A61K,A61P,C12P

Abstract:
A method for preparing chicken sternal cartilage-derived material comprising hydrolyzed collagen type II, comprising the following steps:(a) cutting fresh chicken sternal cartilage to within not less than about 2 mm of the bone; (b) suspending said cartilage in an aqueous solution; (c) treating said cartilage with a proteolytic enzyme to form a hydrolysate having at least 20% of depolymerized chondroitin sulfate and at least 10% of hyaluronic acid, said proteolytic enzyme being capable of hydrolyzing collagen type II to fragments having an average molecular weight of between about 5,500 to about 10,000 daltons; (d) sterilizing said hydrolysate; (e) filtering said hydrolysate; (f) concentrating said hydrolysate; (g) drying said hydrolysate to form a powder enriched in collagen type II powder; and (h) isolating said powder enriched in collagen type II.