Patent ID: 9200309
Filing Date: 2015-12-01
Classification: C12Q,G01N

Abstract:
1. A method for identifying, selecting or testing a compound which inhibits the activity of sphingosine-1-phosphate lyase, comprising: (i) seeding cells which express sphingosine-1-phosphate lyase and culturing the cells; (ii) mixing the cells with a labeled lipid substrate and a test compound and culturing the cells, wherein in step (i), step (ii), or both steps (i) and (ii), the cells are cultured using a culture medium containing vitamin B (iii) lysing the cells or separating the culture supernatant from the cells; (iv) contacting a support material which binds to a phosphorylated lipid with the lysed cells or the culture supernatant; (v) measuring the amount of labeled phosphorylated lipid bound to the support material; and (vi) determining whether the activity of sphingosine-1-phosphate lyase is inhibited wherein an increase in the amount of the labeled phosphorylated lipid compared with the case in which the test compound is not added indicates inhibition of sphingosine-1-phosphate lyase.