Patent ID: 6458562
Filing Date: 2002-10-01
Classification: C07K,C12N,Y10S

Abstract:
A method for producing a hepatitis E virus open-reading frame 2 protein, said method comprising:a) culturing a host cell containing a DNA molecule consisting of nucleotides which encode a hepatitis E virus open-reading frame 2 protein having its amino terminus at amino acid 112 of open reading frame 2 and its carboxy-terminus at an amino acid in the range of amino acids 578-607 of open reading frame 2 under conditions suitable to cause expression of said hepatitis E virus open reading frame 2 protein; b) lysing said host cell to produce a cell lysate; c) fractionating said lysate by anion exchange chromatography to produce a first set of HEV ORF2-positive protein fractions; d) subjecting said first set of HEV-ORF2-positive protein fractions to gel filtration chromatography to produce a second set of HEV ORF2-positive protein fractions; e) subjecting said second set of HEV-ORF2-positive protein fractions to anion exchange chromatography to produce a third set of HEV ORF2-protein positive fractions; and f) subjecting said third set of HEV-ORF2-positive protein fractions to gel filtration chromatography.