Patent ID: 8720546
Filing Date: 2014-05-13
Classification: C09K

Abstract:
1. A method for treatment of a subterranean target site comprising the steps of, in order: a) preparing a biomass suspension comprising one or more species of microorganisms; b) introducing the suspension of (a) into an injection well bore of the subterranean target site; c) optionally introducing one or more fluids into the injection well bore; d) introducing a first nutrient solution formulation into the injection well bore wherein said first nutrient solution supports growth of one or more microorganisms of (a) at said subterranean target site but does not promote biomass aggregation and biofilm production; and e) introducing a second nutrient solution formulation into the injection well bore wherein said second nutrient solution formulation promotes bioplugging of pores and channels in the subterranean target site.