Patent ID: 8986997
Filing Date: 2015-03-24
Classification: C07K,C12N,C12Y

Abstract:
1. A method for identifying stable molecules of interest, comprising: a) Introducing mutations to one or more expression nucleic acids encoding molecules of interest; b) introducing one or more expression constructs encoding a fusion molecule into a plurality of said host cells, wherein said expression construct comprises a first gene encoding said molecules of interest inserted into a second gene that encodes a screenable marker; c) culturing said host cells in a cultured medium; d) exposing said plurality of host cells to a screening condition; e) assessing the stability of said molecules of interest by identifying cells that express a screenable marker, wherein expression of said screenable marker is the result of expression of stable molecules of interest; f) identifying stable molecules of interest by identifying host cells expressing stable molecules of interest, wherein said stable molecules of interest exhibit equal or increased expression of said screenable marker relative to the level of expression when wild type molecules of interest are inserted into said second gene; and g) isolating said host cells expressing said stable molecules of interest.