Patent ID: 8119370
Filing Date: 2012-02-21
Classification: A61K,A61P,C07K,C12N,Y02A

Abstract:
1. A cleavable single-chain polypeptide comprising: a) a first amino acid sequence region comprising i) a first domain comprising a botulinum neurotoxin heavy chain binding element able to preferentially interact with a target cell surface marker under physiological conditions; and ii) a second domain comprising a botulinum neurotoxin heavy chain translocation element able to facilitate the transfer of said single-chain polypeptide across a vesicular membrane; and b) a second amino acid sequence region comprising a botulinum neurotoxin light chain therapeutic element having SNARE-protein sequence-specific endopeptidase activity; c) a third amino acid sequence region comprising a protease cleavage site, wherein the protease cleavage site is cleaved by a non-human Enterokinase or a tobacco etch virus protease; wherein said first and second amino acid sequence regions are separated by said third amino acid sequence region.