Patent ID: 6319713
Filing Date: 2001-11-20
Classification: A61K,C07K,C12N,C12Q,C40B,G01N

Abstract:
A method for making recombined nucleic acids, the method comprising:(a) providing at least one single-stranded polynucleotide;(b) providing one or more nucleic acids, at least one of which differs from the single-stranded polynucleotide(s) in at least one nucleotide, and fragmenting the one or more nucleic acids to produce a plurality of non-identical nucleic acid fragments that are capable of hybridizing to the single-stranded polynucleotide(s);(c) contacting the single-stranded polynucleotide(s) with the plurality of nucleic acid fragments, thereby producing annealed nucleic acid products;(d) contacting the products of (c) with a polymerase; and,(e) contacting the products of (d) with a ligase, thereby producing recombined nucleic acids annealed to the single-stranded polynucleotide(s).