Patent ID: 6815186
Filing Date: 2004-11-09
Classification: A61B,C12Q

Abstract:
A method for producing a stabilized enzyme emulsion for use with a polarographic or amperometric sensor comprising the steps of:making an aqueous solution of a protein, either water soluble enzyme that oxidizes an organic substrate to produce hydrogen peroxide or a carrier protein; emulsifying a volume of a water immiscible oxygen dissolving substance selected from the group consisting of perfluorocarbons, silicone oils, fluorosilicone oils, aromatic and aliphatic hydrocarbon oils or solids, carotenoids and steroids into the aqueous solution to form an emulsion; contacting the emulsion with a protein crosslinking agent; and spreading a mixture of the protein crosslinking agent and the emulsion into a uniform layer whereby the crosslinking agent crosslinks the protein within the emulsion to form a solid gel.