Patent ID: 6297007
Filing Date: 2001-10-02
Classification: C12Q

Abstract:
A method for recovery of DNA comprising a nucleotide sequence of a portion of a gene that encodes a protein that is involved in antibiotic synthesis, from humic materials or lichen, comprising the steps of:(a) combining a DNA sample derived from humic material or lichen with a set of oligonucleotide primers in a reaction mixture which comprises a DNA polymerase and deoxynucleotide triphosphates, wherein the primers are capable of hybridizing to nucleotide sequences that encode conserved regions of a protein, the protein being involved in antibiotic synthesis;(b) amplifying the DNA flanked by the nucleotide sequences that encode conserved regions of the protein in the reaction mixture by polymerase chain reaction; and(c) isolating the amplified DNA from the reaction mixture.