Patent ID: 8232052
Filing Date: 2012-07-31
Classification: A61P,C12Q

Abstract:
1. A method of selecting a dopaminergic neuron proliferative progenitor cell, wherein the method comprises the step of contacting a polynucleotide probe with a cell sample thought to comprise a dopaminergic neuron proliferative progenitor cell, wherein a cell which binds to said polynucleotide probe is identified as a dopaminergenic neuron proliferative progenitor cell, wherein the polynucleotide probe identifies the human Lrp4 gene and comprises a sequence selected from the following nucleotide sequences (a) to (c): (a) a nucleotide sequence complementary to a nucleotide sequence of SEQ ID NO: 2, wherein the polynucleotide probe has at least 95% homology to the sequence of SEQ NO: 2; (b) a nucleotide sequence complementary to a nucleotide sequence encoding an amino acid sequence of SEQ ID NO: 4, wherein the polynucleotide probe has at least 95% homology to the sequence encoding the amino acid sequence of SEQ ID NO: 4; and (c) a nucleotide sequence that hybridizes under stringent conditions with a polynucleotide consisting of a nucleotide sequence of SEQ ID NO: 2; wherein the stringent conditions comprise a wash step comprising a wash 2×SSC, 0.1% SDS at 65° C. wherein the polynucleotide probe had at least 95% homology to the sequence of SEQ ID NO: 2.