Patent ID: 7615229
Filing Date: 2009-11-10
Classification: A61K,A61P,C07K

Abstract:
1. An isolated P4 variant protein of non-typable Haemophilus influenzae (NTHi) that has reduced phosphatase activity compared to wild-type P4 protein and that induces antibody to wild-type P4 protein, said antibody having bactericidal activity against NTHi, wherein the P4 variant protein has a mutation selected from the group consisting of: (a) a mutation at amino acid residue 39 of SEQ ID NO:3, which is a glutamine in wild-type P4 protein; (b) a mutation at amino acid residue 48 of SEQ ID NO:3, which is a phenylalanine in wild-type P4 protein; (c) a mutation at amino acid residue 64 of SEQ ID NO:3, which is an aspartic acid in wild-type P4 protein; (d) a mutation at amino acid residue 161 of SEQ ID NO:3, which is a lysine in wild-type P4 protein; (e) a mutation at amino acid residue 218 of SEQ ID NO:3, which is an asparagine in wild-type P4 protein; (f) mutations at amino acid residues 35 and 37 of SEQ ID NO:3, which are alanine in wild-type P4 protein, where the mutations are not glutamic acid, glutamine or threonine; (g) mutations at amino acid residues 64 and 66 of SEQ ID NO:3, which are aspartic acid in wild-type P4 protein; and (h) combinations of one or more of the mutations of (a)-(g).