Patent ID: 9206409
Filing Date: 2015-12-08
Classification: B01D,C07K,C12N,C12P,C12R,C12Y

Abstract:
1. An animal product free (APF) process for obtaining a botulinum neurotoxin, the process comprising the following steps: (a) providing an APF fermentation medium; (b) fermenting (c) performing a first contacting step wherein the fermentation medium containing the botulinum neurotoxin is contacted with an anion exchange chromatography medium; (d) eluting the captured botulinum neurotoxin from the anion exchange medium to obtain a first eluate; (e) performing a second contacting step in which the first eluate is contacted with a cation exchange chromatography medium to obtain a second eluate; (f) contacting the second eluate with a hydrophobic interaction chromatography medium; and (g) eluting a third eluate from the hydrophobic interaction chromatography medium; thereby obtaining the botulinum neurotoxin.