Patent ID: 8063198
Filing Date: 2011-11-22
Classification: C07H,Y02P

Abstract:
1. A method of removing a silyl protecting group from an oligonucleotide, comprising the step of admixing, under aqueous conditions, an oligonucleotide bearing a silyl protecting group with at least one solvent selected from the group consisting of DMSO, DMF, ethanol, isopropanol, methanol, acetonitrile, and combinations thereof, and a deprotecting reagent selected from the group consisting of pyridine-HF, DMAP-HF (dimethylaminopyridine-HF), urea-HF, TSAF (tris(dimethylamino)sulfonium difluorotrimethylsilane), DAST (diethylamino sulfur trifluoride), polyvinyl pyridine-HF and an amine-HF reagent of formula AA: wherein R R R n is 1 to 20.