Patent ID: 8685413
Filing Date: 2014-04-01
Classification: A61K,A61P,C07K,C12N

Abstract:
1. A Pasteurella multocida ( P. multocida ) mutant strain, comprising a defective fur gene and a defective ompH gene such that expression of fur gene or of ompH1 and ompH2 genes does not occur, wherein the defective fur gene is a wild-type fur gene integrated with an internal nucleotide fragment of 394 base pairs of the wild-type fur gene obtained by amplification with Fur1 (SEQ ID NO: 1) and Fur2 (SEQ ID NO: 2) primers, wherein the defective fur gene is obtained through triparental mating, and the defective OmpH gene has a nonsense mutation at nucleotide position 76 of the oomph1 gene, and a nonsense mutation at nucleotide position 670 of the ompH2 gene.