Patent ID: 8372580
Filing Date: 2013-02-12
Classification: C12Q

Abstract:
1. A method of screening small molecules that complement a breast cancer susceptibility gene 1 (BRCA1) defect comprising the steps of: a) coexpressing Ubc9, SUMO-1, estrogen receptor (ER) α and a reporter in isolated cells comprising a reporter gene under the control of an estrogen responsive element (ERE), wherein the reporter gene is a luciferase gene; b) inducing the cells with estrogen (E2) after transfection; c) treating the cells with a small molecule compound; d) harvesting the cell after sufficient time to allow luciferase expression; and e) measuring luciferase activity from said cells; wherein an increase in luciferase activity in treated cells as compared to non-treated cells indicates that the small molecule is capable of complementing a BRCA1 defect; and wherein a decrease or no effect in luciferase activity in treated cells as compared to non-treated cells indicates that the small molecule is not capable of complementing a BRCA1 defect.