Patent ID: 6069243
Filing Date: 2000-05-30
Classification: C07B,C07H

Abstract:
A method for linking a phosphoramidite nucleoside to a solid support-bound nucleoside comprising the steps of:(a) coupling said phosphoramidite nucleoside to a reactive hydroxyl group of said solid support-bound nucleoside to form a phosphorus nucleoside linkage therebetween; and(b) oxidizing said phosphorus nucleoside linkage with an oxidizing reagent; wherein said solid support is washed with acetonitrile prior to or subsequent to at least one of said coupling or oxidizing steps, said acetonitrile having a water content between 30 ppm and about 1250 ppm, or said oxidizing reagent is in an acetonitrile solvent having a water content between 30 ppm and about 1250 ppm.