Patent ID: 6211351
Filing Date: 2001-04-03
Classification: C07H,C12N

Abstract:
A heteroduplex mutational vector comprising:(a) a first oligonucleobase strand of at least 12 linked nucleobases and not more than 75 linked nucleobases, which first oligonucleobase strand has a first and a second terminal nucleobase;(b) a second oligonucleobase strand having a 3' most and a 5' most nucleobase and having a number of nucleobases equal to the first oligonucleobase strand, which second oligonucleobase strand is optionally divided into a first chain and a second chain; and(c) a single 3' end nucleobase and a single 5' end nucleobase;in which(i) the 3' most and 5' most nucleobases of the second oligonucleobase strand are Watson-Crick base paired to the first terminal and the second terminal nucleobase of the first oligonucleobase strand, respectively,(ii) the 3' most nucleobase of the second oligonucleobase strand and the second terminal nucleobase of the first oligonucleobase strand are protected from 3' exonuclease attack, and(iii) the second oligonucleobase strand contains at least two non-overlapping regions of at least 5 contiguous nucleobases that are Watson-Crick base paired to nucleobases of the first oligonucleobase strand,provided that at least one nucleobase of the first oligonucleobase strand is paired with a non-complementary base of the second oligonucleobase strand.