Patent ID: 6159718
Filing Date: 2000-12-12
Classification: C12G,C12N,C12Y

Abstract:
An isolated and purified DNA sequence which encodes an enzyme exhibiting polygalacturonase activity and which DNA sequence hybridizes to a DNA sequence comprisingACCAAGACAACCCGAAGCTTGAACCCTCTTGGCC (SEQ ID NO: 33) CAAATCCAACAATGCACCTTAACACCACCCTACT CGTCTCGCTCGCCCTCGGGCGCGCGAGCGTCCTC GCCAGCCCAGCCCCACCAGCAATCACGGCCCCGC CCACGGCCGAGGAGATCGCGAAGGCGACGACCTG CACGTTCTCCGGATCGAACGGCGCCTCGTCGGCC AGCAAGTCAAGACCTGTGCTCGACCATGTGTTGT GAATGTGCCGTTCCGTGCG or AAGTCTCGCGCGCACAGCCTGACCAACTCGGTGA (SEQ ID NO: 34) TTCAGCGGCTGAAGATCGTCAACTCGCCGGTGCA GGTTTTCACGTTGCGGGGTCGGATTATCTGACCC TCAAGGATATCACGATCGACAACTCGGACGGCGA CGACAATGGCGGGCATAATACCGATGCGTTTGAT ATCGGCACGAGCACGTATGTCACGATCTCGGGCG CCACGGTGTATAATCAGGATGATTTGCGTGGGCT GTGTAATTCGGGGGAGAATATCTACTTCTCGGGC GGCCTACTGCTCCGGTGGACACGGCTTGTCCATT GGTTCGGTGGGCGGACGCAGTGATAATACGGTTA AGAACGTGACGTTTGTGGATTCGACGATCATTAA CTCAGATAATCGGTCCGCAATCAAAACCAACATC GACACCACCGGCTCCGTGTCCGACGTCACCTACA AGGACATCACGCTCACCTCCATCGCCAAGTACGG GATCGTGGTGCAGCAGAACTACGGCGACACGTCA TCGACGCCCACGACGGGGGTGCCGATCACGGACT TTGTGCTGGACAACGTGCACGGCTCGGTGGTCAG CTCGGGGACCAACATCCTCATCTCGTGCGGGGTC GGGCAGTTGTTCGGATTGGAGTGGACGGATGTGA GTGTCAGTGGGGGGAAGACGAGTTCCAAGTGTAC GAATGTGCCGAGTGGGGCTAGTTGTTGATTCTCT GGTTGTTTGTGGTTGAGAGGGGGAGGGGGGGTGA TTTCTCAAGCTGGAAGGGGTTCTTCGAGCTTAGG AGGTCTCAGGCTTAGTTTGGAGAGCGGAACGGGT CTCTTGACTACTTAGGTTGCTCTTGTTTGAATGG under the following conditions: hybridizing in 5.times. SSC, 5.times. Denhardt's solution, 50 mM sodium phosphate, pH 6.8 and 50 ug denatured sonicated calf thymus DNA for 18 hrs at about 40 C. followed by washing three times in 2.times. SSC, 0.2% SDS at 40 C. for 30 minutes.