Patent ID: 6660309
Filing Date: 2003-12-09
Classification: A61K

Abstract:
A method to isolate a composition which inhibits insulin secretion in &bgr;TC-6 cells and HIT-T15 cells and is non-cytotoxic to &bgr;TC-6 cells, but cytotoxic to HIT-T15 cells andwhich is cytotoxic to SV40 Mes13 cells, but not cytotoxic to MDCK cells, which method comprises a) extracting the bark of Ficus bengalensis with a solvent consisting of lower alcohols to obtain an extract; b) drying the extract to obtain a residue; c) dissolving the residue in methanol; d) loading the dissolved residue onto an activated silica gel column; e) eluting the column with a solvent consisting of ethanol:hexane (2:1) to obtain an eluent; and f) removing said solvent from the eluent to obtain said composition.