Patent ID: 8445194
Filing Date: 2013-05-21
Classification: C07H,C07K,C12Q,G01N,Y10S

Abstract:
1. A method of identifying a sequence of a target polynucleotide, said method comprising: a) providing a substrate comprising a plurality of discrete regions, wherein a plurality of concatemers are randomly disposed on said plurality of said discrete regions, wherein at least a majority of said discrete regions comprises a single concatemer, wherein at least a majority of said concatemers at said plurality of discrete regions are optically resolvable, and wherein each concatemer comprises a plurality of monomeric units and each monomeric unit comprises: b) applying a first set of probes to said substrate such that one or more probes from said first set hybridizes to said adaptor; c) applying a second set of probes to said substrate such that one or more probes from said second set hybridizes to said first target sequence; d) ligating probes from said first set and probes from said second set that are hybridized to adjacent sequences of said monomeric unit to form a ligated complex; e) detecting said ligated complex thereby identifying a nucleotide of said target polynucleotide; f) stripping off said ligated complex; g) repeating steps (b) through (f) in order to determine said sequence of said target polynucleotide.