Patent ID: 7572884
Filing Date: 2009-08-11
Classification: C07K

Abstract:
1. A method for making GLP-1 or a GLP-1 analogue comprising at least one lysine residue being acylated in its ε-amino group, said method comprising the following steps: (i) culturing a host cell comprising a polynucleotide sequence encoding a precursor molecule of the GLP-1 or GLP-1 analogue under suitable conditions for expression of the precursor molecule said precursor molecule comprising an N-terminal extension capable of protecting the GLP-1 or GLP-1 analogue against proteolytic degradation, said N-terminal extension comprising: i) a sequence of Glu-Glu-Ala-Glu(SEQ ID NO:27), and ii) a cleavage site positioned at its C-terminal end for cleavage from the GLP-1 or GLP-1 analogue, wherein the cleavage site is not a Lys; (ii) separating the expressed precursor from the culture broth; (iii) acylating the ε-amino group of at least one lysine residue in the desired polypeptide; (iv) removing the N-terminal extension by chemical and/or enzymatic cleavage and isolating the acylated polypeptide by suitable means, wherein said GLP-1 or GLP-1 analogue is a peptide of GLP-1(7-36) or GLP-1(7-37).