Patent ID: 8785150
Filing Date: 2014-07-22
Classification: G01N

Abstract:
1. A method of determining the amount of a test antigen by immunohistochemistry (IHC), comprising: providing a formalin-fixed, paraffin-embedded (FFPE) cell or tissue sample comprising the test antigen and a quantifiable internal reference standard (QIRS) for the test antigen, the QIRS being a second antigen different from the test antigen, the FFPE sample having been prepared at a time T providing a reference calibration curve comprising at least a ratio that relates the amount of the test antigen to the amount of the QIRS in a reference FFPE sample at a time T measuring a first IHC signal corresponding to the QIRS in the FFPE sample at time T measuring a second IHC signal corresponding to the test analyte in the FFPE sample at time T calculating the amount of the test antigen in the FFPE sample by applying the ratio(s) of the calibration curve as a conversion factor.