Patent ID: 7452718
Filing Date: 2008-11-18
Classification: C12N

Abstract:
1. A direct differentiation method of obtaining cardiomyocyte lineage cells from primate pluripotent stem (pPS) cells, comprising in the following order: a) obtaining undifferentiated pPS cells; b) plating the undifferentiated pPS cells without forming embryoid bodies directly onto a solid surface comprising a substrate to which cardiomyocyte lineage cells adhere without forming embryoid bodies; c) permitting the plated pPS cells to adhere onto the substrate; d) culturing the adhered cells in the absence of serum or feeder cells, but in the presence of activin and a bone morphogenic protein to produce cardiomyocyte lineage cells; e) harvesting the cardiomyocyte lineage cells from the culture.