Patent ID: 7968314
Filing Date: 2011-06-28
Classification: C12Q

Abstract:
1. A method of evaluating whether a test compound is a slow or a rapid acting inhibitor of a target enzyme so as to select time points for a time-dependent inhibition assay, comprising the steps of: a. performing a first assay of the test compound, said first assay comprising the steps of: b. comparing said first and second results to determine whether the test compound is a slow acting inhibitor that substantially inhibits the target enzyme in more than about 10 minutes or a rapid acting inhibitor that inhibits the target enzyme in about 10 minutes or less; c. selecting time points for a secondary inhibition assay of the test compound to determine time-dependent kinetics based on whether the test compound is a slow acting inhibitor or a rapid acting inhibitor; and d. performing the secondary inhibition assay, said secondary inhibition assay comprising the steps of: