Patent ID: 6395517
Filing Date: 2002-05-28
Classification: C12Q

Abstract:
A method useful for detecting a Cryptosporidium parvum organism in an aqueous sample, the method comprising(a) concentrating any Cryptosporidium parvum organism present in the sample by immunomagnetic separation; (b) amplifying a target Cryptosporidium parvum nucleic acid present in the sample using a nucleic acid selected from the group consisting of a nucleic acid having the sequence SEQ ID NO:2 and a nucleic acid having the sequence SEQ ID NO:3, and (c) detecting any amplified target nucleic acid formed in step (b) thereby detecting a Cryptosporidium parvum organism in an aqueous sample; wherein the detection of step (c) comprises using an oligonucleotide probe having the sequence SEQ ID NO:4.