Patent ID: 6500624
Filing Date: 2002-12-31
Classification: C07K

Abstract:
A method of assaying a test ligand for binding to a human CNS receptor, which comprises the steps of incubating the test ligand under appropriate conditions with a human EAA receptor-producing cell, or with a membrane preparation derived therefrom which contains said EAA receptor, and then determining the extent of binding between the human EAA receptor and the test ligand, wherein said cell has been engineered genetically to produce a kainate-binding human EAA receptor having incorporated expressibly therein a heterologous DNA molecule that codes for a human EAA3 receptor selected from the group consisting of:(a) a human EAA3a receptor having the amino acid sequence of SEQ ID NO:2; (b) a human EAA3b receptor having the amino acid sequence of SEQ ID NO:2 with the exception that the amino acid at position 639 is asparagine instead of aspartate; (c) a human EAA3c receptor having the amino acid sequence of SEQ ID NO:18, and (d) a human EAA3d receptor having the amino acid sequence of SEQ ID NO:19.