Patent ID: 6433253
Filing Date: 2002-08-13
Classification: C12N

Abstract:
A process for the production of a transgenic plant with an increased or reduced degree of branching of amylopectin starch, comprising the steps of:a) preparing a DNA comprising: i) a promoter which is active in plants and ensures the formation of an RNA in target tissues or target cells; ii) a DNA sequence from potato, wheat or maize encoding a protein with the enzymatic activity of a debranching enzyme which is detectable by antibodies directed against one or more of peptide sequences â€œSEQ ID NO: 1 to SEQ ID NO. 12â€ ; iii) a 3â€²-non-translated sequence which in a plant cell leads to the transcription termination and to the addition of a poly-A-tails to the 3â€²-end of the RNA; b) transferring and incorporating said DNA molecule into the genome of a plant cell; and c) regenerating intact, whole plants from said plant cell.