Patent ID: 8986976
Filing Date: 2015-03-24
Classification: C07H,C12N

Abstract:
1. A method for isolation of nucleic acid from a sample, said method consisting of the steps of: (a) adding lysis buffer having basic pH to the sample containing nucleic acid to obtain a lysed solution; (b) adding a binding buffer to the lysed solution obtained in step (a) to bind the nucleic acid to a cotton matrix at room temperature, wherein the binding buffer has a pH ranging from 8 to 11; and (c) washing with wash buffer and eluting the cotton matrix bound nucleic acid with an elution buffer to isolate and purify the nucleic acid, wherein the lysis buffer is selected from the group consisting of guanidine thiocyanate, guanidine hydrochloride, EDTA, Tris, detergent, polyol, monovalent salt containing group IA cation, divalent salt containing group IIA cation, protein digesting enzyme and combinations thereof, wherein the sample containing nucleic acid and the lysis buffer are mixed and heated at 50° C. to 95° C. for 1-20 minutes, wherein the lysis buffer has a pH ranging from about 8 to about 11; wherein the binding buffer comprises water; and where the elution buffer comprises water and a buffer or salt, the elution buffer having a temperature ranging from 45° C. to 99° C. and having pH ranging from 8 to 11.