Patent ID: 8709726
Filing Date: 2014-04-29
Classification: C12Q

Abstract:
1. A method for identifying the presence or absence of Y-chromosome nucleic acid in extracellular nucleic acid from a pregnant female, which comprises: (a) contacting under amplification conditions extracellular nucleic acid from a pregnant female with three or more primer sets selected from the group consisting of: wherein (i) the nucleic acid comprises maternal nucleic acid and fetal nucleic acid, (ii) each primer of each primer set hybridizes to Y-chromosome nucleic acid, and (iii) each primer set consists of a first primer and a corresponding second primer; and (b) detecting the presence or absence of amplification products from three or more of the primer sets, whereby the presence of Y-chromosome nucleic acid is based on detecting the presence of the amplification products and whereby the absence of Y-chromosome nucleic acid is based on detecting the absence of the amplification products.