Patent ID: 9074236
Filing Date: 2015-07-07
Classification: C12Q,G01N,H01J

Abstract:
1. A method for identifying one or more unknown microbes, comprising: a) providing a sample containing one or more unknown microbes; b) disrupting the one or more unknown microbes present in the sample to provide a fluid extract; c) separating a soluble protein fraction from an insoluble protein fraction present in the fluid extract and preparing a solution containing soluble, intact proteins from the one or more unknown microbes; d) injecting the solution into an ion source of a mass spectrometer system and ionizing a stream or flow of the solution to form ionized proteins; e) analyzing the ionized proteins with a mass analyzer of the mass spectrometer system, wherein analyzing comprises: f) using information obtained in step (e) for one or more proteins from each of the one or more unknown microbes in the sample to identify at least one of the unknown microbes, wherein steps (b)-(f) are completed in less than 25 minutes.