Patent ID: 6303376
Filing Date: 2001-10-16
Classification: A61P,C07H,C12N,C12Q

Abstract:
A method for targeted mutagenesis of a nucleic acid molecule in a solution or cells comprising the steps of:binding a high affinity, triple-helix forming oligonucleotide to a target region of a double-stranded nucleic acid molecule, wherein the oligonucleotide comprises a single-stranded nucleic acid that forms a triple-stranded nucleic acid molecule not including a mutagenic molecule coupled thereto with the target region; andthe disassociation constant for the oligonucleotide and the target region is less than or equal to 2.times.10.sup.-8,to form a triplex region that results in generation of a site specific mutation in the nucleic acid molecule by cell repair mechanism.