Patent ID: 7049099
Filing Date: 2006-05-23
Classification: C07K

Abstract:
1. A method for producing a Recombinant Human Mannan-Binding Protein (rhMBP) comprising: (a) introducing expression vector pNOW1-hMBP into a Chinese Hamster Ovary (CHO) cell, wherein the CHO cell lacks dihydrofolate reductase; (b) culturing the CHO cell in a culture medium containing neomycin; (c) culturing the CHO cell in a culture medium containing methotrexate; and (d) collecting the rhMBP from the cultured CHO cell possessing resistance to both neomycin and methotrexate, wherein the expression vector pNOW1-hMBP comprises the plasmid pNOW1 and cDNA consisting of the nucleotide sequence of SEQ ID NO:2.