Patent ID: 9090866
Filing Date: 2015-07-28
Classification: B25J,C12N

Abstract:
1. A method for producing a composition of matter comprising a sample of live cells in an agarose containing bead, wherein said bead is coated with agarose, comprising: (a) mixing a first sample of agarose with said sample of live cells to form a suspension; (b) moving said suspension to a first sample of mineral oil, to form a bead from said suspension; (c) removing said bead from said first sample of mineral oil; (d) rinsing mineral oil from said bead; (e) moving said bead with a trumpet tool to a second solution of agarose; (f) coating said bead with said second solution of agarose, (g) removing said bead from said second solution of agarose with a straw tool, and (h) dispensing said coated bead into a second sample of mineral oil, wherein said sample of mineral oil is kept at a temperature gradient so that said bead moves along a path from a location in the mineral oil that is at a higher temperature of about 20° C. to about 30° C. to a location in the mineral oil at a lower temperature of about 0° C. to about −8° C.