Patent ID: 8569003
Filing Date: 2013-10-29
Classification: C07K,C12N,C12Q,G01N

Abstract:
1. A method for determining a substance that specifically binds to a ligand, which comprises binding a test substance via the ligand to a complex comprising a fusion protein comprising: (1) a first region selected from the group consisting of (a) to (d) below: (2) a second region consisting of an amino acid sequence for a polypeptide having at least one cysteine residue for binding to other useful compound via its thiol group, wherein the second region is selected from the group consisting of (e) to (h) below: a ligand bound to the fusion protein via the thiol group of the cysteine residue in the second region and detecting light being emitted from the fusion protein in the presence of a luciferin to determine the substance.