Patent ID: 8067177
Filing Date: 2011-11-29
Classification: C12Q

Abstract:
1. A method for detecting polynucleotide amplification of a target sequence, comprising: (a) providing a mixture comprising a sample containing a target sequence, with one or more than one oligonucleotide primers complementary to regions of the target sequence, a polymerizing enzyme, nucleotide substrates, and an oligonucleotide probe comprising a 5′ end and a 3′ end and two or more labels, wherein the oligonucleotide probe is an oligonucleotide conjugate, wherein one of the labels is a fluorophore and one of the labels is a quencher, wherein the oligonucleotide conjugate comprises an oligonucleotide or a modified oligonucleotide, wherein the oligonucleotide or modified oligonucleotide sequence has an overlap of from 1 to 7 bases with at least one of said primer sequences and the oligonucleotide or modified oligonucleotide has a sequence complementary to a portion of the target sequence being amplified; (b) incubating the mixture under conditions favorable for polymerization and amplification of the target sequence; and (c) detecting the amplification of the target sequence.