Patent ID: 6811983
Filing Date: 2004-11-02
Classification: C12Q,G01N

Abstract:
A method of assaying potential inhibitors of EBNA-1 comprising the steps of:(a) obtaining an EBV-positive cell line and an EBV-negative cell line wherein both cell lines are proliferative B-lymphocyte cell lines; (b) treating the cell lines with a test compound by exposure of the cells to the test compound in solution or to the test compound as expressed or provided by an intracellular gene construct, and (c) examining either cell proliferation or induction of apoptosis in the cell lines, wherein significant apoptosis or decrease in cell proliferation in the EBV-positive cell line and lack of significant apoptosis or increase in cell proliferation in the EBV-negative cell line indicates that the compound specifically inhibits EBNA-1.