Patent ID: 9200323
Filing Date: 2015-12-01
Classification: C12Q

Abstract:
1. A process for hybridizing an oligonucleotide to a first nucleic acid target, the method comprising the steps of: providing an oligonucleotide comprising a nucleic acid sequence complementary in part to the first target, but comprising at least one artificial mismatch relative to the first target and comprising a nucleic acid sequence complementary in part to a second target, but comprising at least one artificial mismatch and a true mismatch relative to the second target, wherein the true mismatch and the artificial mismatch are separated from one another by six to nine nucleotide positions and wherein the artificial mismatch is a naturally occurring nucleotide base; and combining the oligonucleotide and the first and second targets under a hybridization condition that allows formation of (a) a first duplex comprising the oligonucleotide and the first target, and (b) a second duplex comprising the oligonucleotide and the second target, wherein the first duplex has a melting temperature 4-10° C. higher than that of the second duplex.