Patent ID: 6511802
Filing Date: 2003-01-28
Classification: C12N,C12Q

Abstract:
A method of analyzing differential gene expression, comprising:providing a reference population of nucleic acid sequences attached to seperate solid phase supports in clonal subpopulations; providing a population of polynucleotides of expressed genes from a first cell or tissue source and at least one population of polynucleotides of expressed genes from a different cell or tissue source, the polynucleotides of expressed genes from each source comprising a light-generating label different from the label comprised by polynucleotides of any other source; competitively hybridizing of polynucleotides of expressed genes from each source with the reference nucleic acid population to form duplexes between the nucleic acid sequences of the reference nucleic acid population and the polynucleotides of each source of each source such that the polytides form the same gene of the different sources hybridize with the same complementary sequence of the reference nucleic acid population and such that such polynucleotides are present in duplexes on each of the solid phase supports in ratios directly related to the relative expression of their corresponding genes on the sources; and detecting a relative optical signal generated by the light-generating labels of the duplexes attached thereto.