Patent ID: 7683236
Filing Date: 2010-03-23
Classification: C12N

Abstract:
1. A method for preparing a non-primate mammalian blastocyst capable of developing into a live-born animal, comprising: a) providing genetically identical reconstructed non-primate mammalian embryos; b) removing the zona pellucida of said reconstructed embryos; c) forming a homologous aggregate of at least two of the zona-free embryos of step b); and d) culturing the homologous embryo aggregate of c) under conditions which result in the formation of a non-primate mammalian blastocyst; wherein said reconstructed embryos are prepared from reconstructed non-primate mammalian oocytes comprising donor cell nuclei or donor nuclear DNA; wherein said donor cell is a non-primate mammalian embryonic stem cell or a non-primate mammalian embryonic germ cell; and wherein said at least two zona-free embryos in step c) are at the four-cell stage.