Patent ID: 6573044
Filing Date: 2003-06-03
Classification: A61K,C07D

Abstract:
A method of determining the identity of proteins that modulate cell proliferation during or after exposure to chemical or genetic changes, said method comprising isolating mRNA transcripts generated from cells after exposure to a compound known to modulate cellular proliferation by hybridizing under stringent conditions to at least one oligonucleotide complementary to a nucleic acid sequence which encodes a protein associated with cell proliferation, isolating mRNA transcripts generated from cells not exposed to said compound by hybridizing to the oligonucleotides, comparing the total number of mRNA transcripts from both treated and untreated cells, and determining which proteins are encoded by mRNA transcripts present in differing amounts in treated or untreated cells, wherein said compound has the following structure: wherein R1 is selected from the group consisting of H and NH2; R2 is selected from the group consisting of H, CO2H, OH and halogen; and R3 is selected from the group consisting of CO2H, NH2 and halogen.