Patent ID: 8399217
Filing Date: 2013-03-19
Classification: C12N,C12P

Abstract:
1. A method for promoting auto-induction, in bacterial cells grown batchwise, of a cloned gene 1 of bacteriophage T7, which gene is under the control of an inducible promoter selected from the group consisting of a lac promoter and an araBAD promoter, and which gene is adapted for stable propagation in said cells, the method comprising: a) providing a culture medium comprising: b) inoculating the culture medium with an inoculum of said bacterial cells; c) incubating the culture of step b) under conditions appropriate for growth of the bacterial cells; and d) continuing to incubate the culture of step c) until depletion of the concentration of said one or more constituents has promoted auto-induction of gene 1 by said inducer.