Patent ID: 7977080
Filing Date: 2011-07-12
Classification: A61P,C07K,C12N,C12P

Abstract:
1. An isolated, synthetic or recombinant nucleic acid comprising (i) a nucleic acid having at least 95%, 96%, 97%, 98%, 99%, or more or complete sequence identity to wherein the nucleic acid encodes at least one polypeptide having a phospholipase C activity; (ii) the nucleic acid of (i), wherein the nucleic acid encodes: SEQ ID NO:2, minus amino acids 1-37, and having at least one amino acid modification selected from the group consisting of N63D, N131S and N134D, or having at least two of the amino acid modifications selected from the group consisting of N63D, N131S and N134D; (iii) a fragment of the nucleic acid of (i) or (ii), wherein the fragment encodes a polypeptide having a phospholipase C activity; (iv) the nucleic acid of any of (i) to (iii), wherein the phospholipase C activity comprises catalyzing hydrolysis of a glycerolphosphate ester linkage in phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylinositol (PI), and/or phosphatidic acid or a combination thereof; (v) the nucleic acid of any of (i) to (iv), wherein the nucleic acid further comprises a heterologous nucleic acid sequence; (vi) the nucleic acid of (v), wherein the heterologous nucleic acid sequence encodes a heterologous polypeptide; (vii) the nucleic acid of (vi), wherein the heterologous polypeptide comprises or consists of a signal sequence (a signal peptide), a catalytic domain, a linker sequence, a protease cleavage recognition site, a non-phospholipase enzyme sequence, a phospholipase enzyme sequence; or (viii) a nucleic acid comprising a sequence completely (fully) complementary to the nucleic acid sequence of any of (i) to (vii).