Patent ID: 6162610
Filing Date: 2000-12-19
Classification: C07D,C12Q,G01N,Y10S

Abstract:
A chemiluminescent substrate for the detection of horseradish peroxidase comprising a xanthan ester of the following formula: ##STR10## wherein ##STR11## an alkyl or branched alkyl group of 1-20 carbon atoms which is substituted with one or more fluorine atoms or any leaving group which allows the production of light from the xanthan ester by reaction with a peroxide and a peroxidase;R.sub.2 and R.sub.3 may be attached to any free carbon atom on the xanthene ring and may independently be hydrogen, alkyl, branched alkyl, substituted alkyl, alkoxy, each containing 1-20 carbon atoms, halogen, nitro, amino, acylamino, carboxamide, carboxylic ester, aryl, heteroaryl, aryloxy or any fluorophore which will exhibit efficient energy transfer from an excited xanthone moiety; ##STR12## may be any nitrogen containing heterocyclic ring which functions as a leaving group and which can also contain carbon, other heteroatoms or additional fused rings, wherein the N atom is bonded to the carbonyl group of the xanthan ester and wherein the ring may also be substituted with R.sub.2 and/or R.sub.3 groups as defined above;Ar is a substituted or unsubstituted aryl group;R' is an alkyl, aryl or substituted aryl group;Y is a substituted or unsubstituted aryl sulfonyl or alkyl sulfonyl group, wherein the S atom is bonded to the N atom of the ##STR13## X is hydrogen or any enzymatically cleavable group; and Z is O or S.