Patent ID: 8058000
Filing Date: 2011-11-15
Classification: C12Q

Abstract:
1. A method for identifying differences between communities of microorganisms comprising: a) obtaining genomic DNA extracts from a first group of individual animal fecal samples and mixing these samples to create a first fecal microbial community DNA composite designated tester; b) obtaining genomic DNA fragments from a second group of animal fecal samples and using these samples to create a second fecal microbial community DNA composite designated blocker; c) labeling the tester and incubating the tester with the blocker d) obtaining labeled first genomic DNA fragments from a first community and hybridizing the first genomic DNA fragments with second genomic DNA fragments from a second community; e) incubating the first and second genomic fragments with additional genomic fragments from the first community containing defined terminal sequence tags to form DNA hybrids; f) capturing the resulting DNA hybrids formed with tags and PCR amplification of only the tagged fragments; g) obtaining enriched amounts of sequences unique to the first community; and h) identifying the enriched sequences thereby identifying the differences between the communities of microorganisms.