Patent ID: 8884214
Filing Date: 2014-11-11
Classification: C07K,G01N,H01J,Y10T

Abstract:
1. A method for identifying and quantifying a peptide in a sample, the method comprising: (a) adding a plurality of halogenated peptide standards to the sample as internal standards; (b) analyzing the sample containing said internal standards using liquid chromatography-mass spectrometry; (c) identifying the liquid chromatography-mass spectrometry data which correspond to the peptide; (d) identifying the liquid chromatography-mass spectrometry data which correspond to the halogenated peptide standards; and (e) quantifying the amount of the peptide in the sample using the data identified in steps (c) and (d), wherein in said halogenated peptides, said halogens are selected from chlorine and bromine, that replace one or more hydrogens in an amino acid, and wherein each halogenated amino acid residue is halogenated phenylalanine.