Patent ID: 8592781
Filing Date: 2013-11-26
Classification: G01K,G01N

Abstract:
1. A method for quantitative evaluation of a wall zeta potential, pH, or temperature distribution, the method comprising: a step of mixing a first fluorescent dye and a second fluorescent dye into a solution, the first dye being positively ionized in the solution, and the second dye being negatively ionized in the solution and having a different fluorescence wavelength from the first dye; a step of flowing the solution onto a measured surface; a step of exciting the measured surface with an evanescent wave to thereby produce a fluorescence intensity distribution of two colors according to a concentration distribution of each fluorescent dye; a step of measuring a fluorescence intensity of the measured surface using a two-dimensional imaging element, the element being capable of providing a fluorescence intensity of each color separated from fluorescence intensities of the two colors; a step of calculating a ratio of the fluorescence intensities of the two colors; and a step of using a previously created equation expressing a relationship between the ratio of fluorescence intensities and a wall zeta potential, pH, or temperature to convert the distribution of the ratio of fluorescence intensities into a two-dimensional distribution of wall zeta potentials, pHs, or temperatures.