Patent ID: 6013494
Filing Date: 2000-01-11
Classification: C12N,C12P,Y10S

Abstract:
A method for the production of 1,3-propanediol from a recombinant microorganism comprising:(i) transforming a suitable host microorganism with one or more transformation cassettes each of which comprises at least one of(a) a gene encoding a glycerol-3-phosphate dehydrogenase activity;(b) a gene encoding a glycerol-3-phosphatase activity;(c) genes encoding a dehydratase activity; and(d) a gene encoding 1,3-propanediol oxidoreductase activity,wherein all of the genes of (a)-(d) are introduced into the host microorganism;(ii) culturing the transformed host microorganism under suitable conditions in the presence of at least one carbon source selected from the group consisting of monosaccharides, oligosaccharides, polysaccharides, or a one-carbon substrate whereby 1,3-propanediol is produced; and(iii) recovering the 1,3-propanediol.