Patent ID: 7821640
Filing Date: 2010-10-26
Classification: A61B

Abstract:
1. A method for reconstructing a three-dimensional fluorescence optical tomography image to examine a plate-shaped object having a first face and an opposite second face and comprising fluorophores, said method comprising: a first illumination step for a sequential illumination of the first face of the object with an excitation light of the fluorophores from a plurality of lighting points, the first illumination step comprising a succession of illumination phases from a lighting point associated with each phase, and a first acquisition step for a sequential acquisition of a first series of images, an image of said series being formed, at each illumination phase of the first face, by a detector comprising a plurality of detection points simultaneously detecting light emitted by the second face of the object, the density of the lighting points being lower than the density of the detection points, a second illumination step, comprising a succession of illumination phases, for a sequential illumination of the second face of the object with a fluorophore excitation light, a second acquisition step for a sequential acquisition of a second series of images, an image of said second series being formed, at each illumination phase of the second face, by detection of light emitted by the first face of the object and reconstruction of the three-dimensional image of the distribution of the fluorophores in the object by means of the first and second series of images.