Patent ID: 6733746
Filing Date: 2004-05-11
Classification: A61P,C12N

Abstract:
A serum-free, eukaryotic cell culture medium comprising the ingredients N-acetyl-L-cysteine, 2-mercaptoethanol, human serum albumin, D,L-tocopherol acetate, soluble human lipids for serum-free media, ethanolamine, human zinc insulin, iron-saturated transferrin, Se4+, hydrocortisone, Ca2+, K+, Mg2+, Na+, Co32âˆ’, PO43âˆ’, D-glucose, HEPES, sodium pyruvate, phenol red, glycine, L-alanine, L-asparagine, L-cysteine, L-aspartic acid, L-glutamic acid, L-phenylalanine, L-histidine, L-isoleucine, L-lysine, L-leucine, L-glutamine, L-arginine HCL, L-methionine, L-proline, L-hydroxyproline, L-serine, L-threonine, L-tryptophan, L-tyrosine, L-valine, biotin, D-calcium pantothenate, choline chloride, folic acid, i-inositol, niacinamide, pyridoxal HCl, riboflavin, thiamine HCl, and vitamin B12,wherein each of said ingredients is present in said medium at a concentration that supports the expansion of CD34+ hematopoietic cells in suspension culture in the absence of stromal cells.