Patent ID: 7960155
Filing Date: 2011-06-14
Classification: C12N,C12P,C12Q,G01N

Abstract:
1. A process for the microbiological oxidation of a substrate compound having an N-, O- or S-heterocyclic mono- or polynuclear aromatic moiety, which process comprises oxidizing at least one aromatic C—H group of the heterocyclic aromatic moiety by wherein the monooxygenase is derived from cytochrome P450 monooxygenase BM-3 from and the mutation consists of a functional mutation in one, two or all of sequence positions 74, 87 and 188, whereby Phe87 is replaced by Ala, Val or Leu, Leu188 is replaced by Asn or Gln, and/or Ala74 is replaced by Val or Gly.