Patent ID: 6017694
Filing Date: 2000-01-25
Classification: C12Q

Abstract:
A method of screening for modulators of respiratory syncytial virus (RSV) matrix protein interaction comprising the steps of:(a) culturing a host cell carrying a nucleic acid sequence coding (1) an RSV matrix protein or an N-terminal fragment of said RSV matrix protein having at least 183 amino acids fused to (2) a protein selected from the group consisting of maltose binding protein (MBP) and glutathione-S-transferase;(b) expressing said fused RSV matrix protein or said fused RSV matrix protein fragment in the cultured host cell of step (a);(c) measuring the interaction of said fused RSV matrix protein or fused RSV matrix protein fragment expressed in step (b); and(d) adding a test sample to said expressed fused RSV matrix protein or expressed fused RSV matrix protein fragment of step (c) and measuring the effect on RSV matrix protein interaction, wherein an increase in the RSV matrix protein interaction indicates the test sample promotes RSV matrix protein interaction and a decrease in the RSV matrix protein interaction indicates the test sample inhibits RSV matrix protein interaction.