Patent ID: 7435586
Filing Date: 2008-10-14
Classification: A61K,A61P,C12N

Abstract:
1. A method for generating a substantially homogeneous population of undifferentiated neural stem cells (NSCs) which are phenotypically peanut agglutinin (PNA) low and heat stable antigen (HSA) low (PNA lo HSA lo ), from cells of the central nervous system (CNS), said method comprising subjecting said cells of the CNS to tissue-disruption means to provide a mixed population of cells comprising the undifferentiated cells to be purified, subjecting said mixed population of cells to cell size-discrimination means to generate a population of cells wherein substantially all cells are between 5 and 50 microns in size, and subjecting said population of cells to cell surface marker-discrimination means to generate a substantially homogeneous population of undifferentiated NSCs which are phenotypically PNA lo HSA lo .