Patent ID: 7700751
Filing Date: 2010-04-20
Classification: A61K,C07K

Abstract:
1. An isolated nucleic acid molecule comprising a nucleotide sequence encoding a humanized immunoglobulin light chain, or variable region thereof, the light chain or variable region comprising: (a) the complementarity determining regions (CDRs) from the 3D6 immunoglobulin light chain variable region sequence set forth as SEQ ID NO:2, and (b) a variable region framework from a human acceptor immunoglobulin light chain sequence, provided that at least one framework residue is substituted with the corresponding amino acid residue from the mouse 3D6 light chain variable region sequence, wherein the framework residue is selected from the group consisting of: (i) a residue that non-covalently binds antigen directly; (ii) a residue adjacent to a CDR; (iii) a CDR-interacting residue; and (iv) a residue participating in the VL-VH interface.