Patent ID: 6524790
Filing Date: 2003-02-25
Classification: B01J,B01L,G01N,Y10S

Abstract:
A method of determining concentration of a reaction or assay product (Cp) in a microfluidic device, the method comprising:(i) converting a labeled first reactant or assay component having a velocity (Ur) and a label (Lr) and a concentration (Cr), the labeled first reactant or assay component producing a signal (Sas) in a signal detection system, to a reaction or assay product comprising a label Lp, having a velocity (Up), wherein (Ur) does not equal (Up); (ii) detecting a resulting change in Sas, which change is dependent on a difference between Up and Ur, wherein the change in Sas correlates to a ratio of Ur to Up or a ratio of Cp to Cr; and, (iii) determining Cp from the ratio of Cp to Cr, or Ur to Up.