Patent ID: 7108976
Filing Date: 2006-09-19
Classification: C12Q

Abstract:
1. A method for genotyping one or more polymorphisms in a nucleic acid sample comprising: fragmenting the nucleic acid sample to generate fragments; ligating an adapter to the fragments said adapter comprising a first priming sequence; hybridizing a collection of capture probes to the fragments, wherein said capture probes comprise in the following order from 5′ to 3′: a second priming sequence that is common to the capture probes in the collection, a tag sequence unique for each species of capture probe, a first target specific sequence, a Type IIs restriction enzyme recognition sequence, and a second target specific sequence wherein the Type IIs restriction enzyme recognition sequence is positioned so that the enzyme will cut on the 5′ side of a polymorphic base; extending said capture probes to generate extended capture probes in a first extension reaction; amplifying the extended capture probes with primers to said first and second priming sequences; digesting the amplified product with a Type IIs restriction enzyme to generate amplified product fragments; extending the amplified product fragments in at least one second extension reaction; hybridizing each second extension reaction to an array comprising tag probes that hybridize to the tag sequences in the capture probes; and analyzing the hybridization pattern on each of the arrays to determine the genotype of one or more polymorphisms in the nucleic acid sample.