Patent ID: 8318458
Filing Date: 2012-11-27
Classification: C12N,C12Y

Abstract:
1. A method for degrading cellulose- and hemicellulose-containing biomass, comprising treating the biomass with a recombinant host cell comprising a nucleic acid construct comprising a polynucleotide encoding a polypeptide having beta-glucosidase activity and recovering the degraded biomass, wherein the polypeptide having beta-glucosidase activity is selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence having at least 95% identity with amino acids 20 to 863 of SEQ ID NO: 2; and (b) a polypeptide which is encoded by a polynucleotide which hybridizes under at least high stringency conditions with (i) nucleotides 58 to 2580 of SEQ ID NO: 1, (ii) the cDNA sequence of nucleotides 58 to 2580 of SEQ ID NO: 1, or (iii) the full-length complement of (i) or (ii), wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.