Patent ID: 6033673
Filing Date: 2000-03-07
Classification: A61K,A61P,C07K,G01N,Y10S

Abstract:
A composition comprising a mutant E. coli heat-labile enterotoxin holotoxin, in which arginine at amino acid position 192 is replaced with glycine and in which leucine at amino acid position 211 is replaced with alanine, which holotoxin has immunologic adjuvant activity and is:(a) substantially less toxic than native E. coli heat-labile enterotoxin holotoxin as measured in the Y-1 adrenal cell assay,(b) substantially less toxic than native E. coli heat-labile enterotoxin holotoxin as measured in the patent mouse assay, and(c) less toxic than a composition consisting of isolated LT(R192G) as measured in the patent mouse assay.