Patent ID: 9221883
Filing Date: 2015-12-29
Classification: C07K,C12N,C12Q,Y02A

Abstract:
1. A method for isolating or recovering from a sample containing nucleic acid sequences a nucleotide sequence encoding a polypeptide with a pesticidal activity comprising: (a) providing a polynucleotide probe comprising the complement of the nucleic acid sequence of any of SEQ ID NO: 1, 9, 10, 11 or 12, or a fragment thereof comprising at least 500 consecutive nucleotides of SEQ ID NO: 1, 9, 10, 11 or 12; (b) contacting the sample with the polynucleotide probe under stringent hybridization conditions, wherein said conditions comprise hybridization for 4 to 12 hours in 50% formamide, 1 M NaCl, 1% SDS at 37° C., and a wash in 0.1×SSC at 60 to 65° C.; (c) isolating or recovering from said sample a nucleic acid that specifically hybridizes with the polynucleotide probe of (a); and (d) assaying the polypeptide encoded by the nucleic acid of step (c) for pesticidal activity.