Patent ID: 8043802
Filing Date: 2011-10-25
Classification: C12Q,Y10S,Y10T

Abstract:
1. A method of detecting the presence of an ion in the presence of other ions, in a sample, comprising: forming a mixture comprising: (1) a nucleic acid enzyme, (2) the sample, and (3) a substrate, the substrate and the nucleic acid enzyme comprising separate nucleic acid strands, to produce a cleavage product from the mixture; and determining the presence of the cleavage product; wherein the enzyme comprises at least one quencher and is dependent on the ion to produce the cleavage product from the substrate, the substrate comprises a ribonucleotide, at least one fluorophore and at least one quencher, when the substrate is hybridized to the nucleic acid enzyme, the first quencher quenches the at least one fluorophore, and when the substrate is not hybridized to the nucleic acid enzyme, the second quencher quenches the at least one fluorophore.