Patent ID: 6706498
Filing Date: 2004-03-16
Classification: C12N

Abstract:
A method of isolating high molecular weight nucleic acid from a biological material which comprises mechanically releasing the high molecular weight nucleic acid from the material by the application of rapidly oscillating reciprocal mechanical energy to the material in the presence of a liquid medium an a closed container to produce a released high molecular weight nucleic acid solution, wherein the released high molecular weight nucleic acid solution has an average molecular weight greater than 10 kilobases, the liquid medium contains one or more particles and detergent in an amount of from 0.1% to 10% weight per weight (w/w), wherein when the liquid medium contains more than one particle, the particles are identical or vary in size, shape, and density, and wherein the application of the energy is conducted by subjecting the container and thereby the material to oscillations at an oscillatory rate of between about 25 hertz (Hz) to about 166 Hz for a period of time of between about 3 seconds to about 5 minutes, the method further comprising recovering the high molecular weight nucleic acid from the liquid medium.