Patent ID: 7033758
Filing Date: 2006-04-25
Classification: C12Q

Abstract:
1. A method for in situ detection of a nucleic acid analyte within a sample of biological material using bDNA hybridization comprising the steps of: (a) preparing the sample of biological material by: (b) contacting the biological material with a target oligonucleotide probe under hybridizing conditions, wherein at least a portion of the target probe is complementary to at least a portion of the nucleic acid analyte, so that an analyte-target probe complex is formed when the nucleic acid analyte is present in the sample; (c) washing the biological material with a washing fluid comprising a detergent, at a temperature in the range of approximately 21 to 60° C.; and (d) detecting any analyte-target probe complex on the substrate by: wherein the nucleic acid analyte is selected from the group consisting of DNA, endogenous genes, and segments thereof.