Patent ID: 6164281
Filing Date: 2000-12-26
Classification: A61K

Abstract:
A method of making, preventing, and reversing pathologic vessel condition, new vessel growth, vessel leakage, or macular edema comprises the steps of:a) selecting a tissue from deceased patient who has said pathologic vessel condition, new vessel growth, vessel leakage, or macular edema,b) cutting said tissue into small pieces approximately 0.1-5 mm.sup.2 by size, or .ltoreq.1 mm by thickness,c) selecting and opening a hamster cheek pouch, and grafting said tissue pieces into underdermal loose connective tissue of said hamster cheek pouch and closing said opening by said hamster's skin,d) maintaining b) and c) under sterilized condition,e) maintaining b) and c) in a temperature approximately 1.degree. C. to 24 .degree. C., prefer 1.degree. C. to 17.degree. C.,f) accelerating neovascularization in the interface between the graft and recipient and the adjacent area, by administering physical stimulation of operation, EDTA treatment, trypsin digestion, cycled glycemia frustration, glycation end product injection, oxygen stimulation, anticoagulation perfusion, heparin treatment, toxin, cancer exudate, AID exudate, or combinations thereof,g) optionally providing a pathologic environment-like condition for facilitating neovascularization,h) administering a testing material, compound, or angiostatic drug to affect neovascularization.