Patent ID: 6811979
Filing Date: 2004-11-02
Classification: C07H,C40B

Abstract:
A conjugate comprising a dye labeled nucleobase of the form:(1) B-L-D, wherein B is a nucleobase, L is an anionic linker, and D comprises at least one fluorescent dye that comprises a xanthene a rhodamine or a fluorescein, or (2) B-L1-D1-L2-D2, wherein B is a nucleobase, L1 and L2 axe linkers such that at least one of L1 and L2 is an anionic linker, and D1 and D2 are members of a fluorescent donor/acceptor pair, such that one of D1 and D2 is a donor dye capable of absorbing light at a first wavelength and emitting energy m response thereto, and the other of D1 and D2 is an acceptor dye capable of absorbing energy emitted by the donor dye and fluorescing at a second wavelength in response thereto, and at least one of D1 and D2 comprises a xanthene, a rhodamine or a fluorescein, wherein L or at least one of L1 and L2 comprises at least one anionic phosphate or anionic phosphonate.