Patent ID: 6545123
Filing Date: 2003-04-08
Classification: A61K,C07H,C07K

Abstract:
A process for making a glycosulfopeptide compound, comprising:(a) providing a presynthesized peptide comprising at least one sulfated tyrosine residue and at least one amino acid residue having a GalNAc side group linked thereto via an N- or O-linkage; (b) providing a purified core 1 &bgr; 1,3-galactosyltransferase and enzymatically linking a Gal to the GalNAc via the core 1  &bgr;1,3-galactosyltransferase; (c) enzymatically linking a GlcNAc to the GalNAc via a N-acetylglucosaminyltransferase; (d) enzymatically linking a second Gal to the GlcNAc via a &bgr; 1,4-galactosyltransferase; (e) enzymatically linking a sialic acid to the second Gal via an &agr; 2,3-sialyltransferase; and (f) enzymatically linking a Fuc to the GlcNAc via an &agr; 1,3-fucosyltransferase.