Patent ID: 6682938
Filing Date: 2004-01-27
Classification: A61B,C07F,C09B,G01N

Abstract:
A method of making a fluorescent compound that emits a signal that can be correlated to an analyte concentration, wherein the compound has the general formula: wherein R1 is selected form the group consisting of hydrogen, lower aliphatic and aromatic functional groups; wherein R2 and R4 are optional functional selected from the group consisting of hydrogen, lower aliphatic and aromatic functional groups and groups that form covalent bonds to a polymer matrix; wherein L1 and L2 are optional linking groups having from zero to four atoms selected from the group consisting of nitrogen, carbon, oxygen, sulfur, and phosphorus; wherein Z is a heteroatom selected from the group consisting of nitrogen, sulfur, oxygen and phosphorus; wherein R3 is an optional group selected from the group consisting of hydrogen, lower aliphatic and aromatic functional groups and groups that form covalent bonds to a polymer matrix; wherein F is a fluorophore with selected molecular properties; the method comprising selecting F and Z to satisfy the following equation such that &Dgr;G, the free energy for electron transfer, is less than about 3.0 kcal molâˆ’1: &Dgr;G=23.06[E0(Zoxidation)âˆ’E0(Freduction)]âˆ’&Dgr;E00], wherein E0(Zoxidation) is the oxidation potential of Z, E0(Freduction) is the reduction potential of F, and &Dgr;E00 is the energy of F in the excited state.