Patent ID: 8354223
Filing Date: 2013-01-15
Classification: C12N,C12Q,G01N

Abstract:
1. A method for identifying an agent that modulates methylation of VEGFR1 by SMYD3, said method comprising the steps of: a. contacting an SMYD3 polypeptide having a methyltransferase activity selected from the group consisting of: i. a polypeptide comprising the amino acid sequence of SEQ ID NO: 2; and ii. a polypeptide that comprises the amino acid sequence having at least 95% identity to SEQ ID NO: 2, wherein said polypeptide comprises the amino acid sequence of positions 100-250 of the amino acid sequence of SEQ ID NO: 2, and has a methyltransferase activity equivalent to the polypeptide consisting of the amino acid sequence of SEQ ID NO:2; with a VEGFR1 peptide to be methylated and a cofactor in the presence of the agent under conditions suitable for methylation of the VEGFR1 peptide, wherein said VEGFR1 peptide is selected from the group consisting of I. a VEGFR1 polypeptide comprising the amino acid sequence SEQ ID NO: 4; II. polypeptide fragment of I, wherein said fragment comprises the amino acid sequence of positions 800-1000; and III. VEGFR1 mutant, wherein said mutant comprises the amino acid sequence of SEQ ID NO:4 including any one of the following mutations: K819A, K819E and K819R; b. detecting the methylation level of the VEGFR1 peptide; and c. comparing the methylation level of step (b) with a control level detected in the absence of the agent, wherein an increase or decrease in the methylation level compared to the control level indicates that the agent modulates the methylation of VEGFR1 by SMYD3.