Patent ID: 7723067
Filing Date: 2010-05-25
Classification: C07K,C12N,C12Y

Abstract:
1. A method for producing a transglutaminase of Streptoverticillium mobaraense which comprises: (a) culturing a coryneform bacterium which comprises a genetic expression construct comprising a promoter immediately linked at its 3′-end to a nucleic acid sequence encoding a signal peptide comprising the amino acid sequence of SEQ ID NO: 2 wherein said nucleic acid sequence encoding a signal peptide domain is immediately linked at its 3′-end to a nucleic acid sequence encoding a transglutaminase of (b) expressing and secreting said transglutaminase containing the pro-structure, wherein the yield of said transglutaminase containing the pro-structure is at least 74 mg/L; (c) cleaving and eliminating the pro-structure part from said transglutaminase containing the pro-structure.