Patent ID: 6153406
Filing Date: 2000-11-28
Classification: A61K,A61P,C07K,C12N

Abstract:
A method for the high level expression of an outer membrane protein P2 from Haemophilus influenzae type b (Hib-P2) in E. coli comprising:(a) transforming E. coli host cells with a vector comprising a selectable marker and a gene coding for a P2 protein selected from the group consisting of(i) a mature P2 protein, and(ii) a fusion protein comprising a mature P2 protein fused to amino acids 1 to 22 of the T7 gene .phi.10 capsid protein (SID NO: 10);wherein said gene is operably linked to a T7 promoter; and(b) growing said transformed E. coli in media containing glucose and a selection agent at about 30.degree. C.; whereby said P2 protein is expressed and is found in inclusion bodies, wherein said P2 protein comprises more than about 2% of the total protein expressed in said E. coli.