Patent ID: 9187543
Filing Date: 2015-11-17
Classification: A61K,C07K,C12P

Abstract:
1. A method for producing a recombinant Type 1 interferon protein, said method comprising: expressing the recombinant interferon protein by culturing a lysing the host cell; obtaining an insoluble fraction and a soluble fraction from the lysed host cell; extracting the insoluble fraction by subjecting it to non-denaturing extraction conditions, wherein the non-denaturing extraction conditions comprise a Zwitterionic detergent at a concentration of about 0.5% to about 2%; obtaining an extract pellet and an extract supernatant from the extracted insoluble fraction; and measuring the activity of the recombinant interferon protein in the extract supernatant, wherein about 80% to about 100% of the recombinant interferon protein in the extract supernatant is determined to be active, without being further subjected to a renaturing or refolding step, and wherein the Type I interferon protein is expressed in the cytoplasm of the host cell.