Patent ID: 8455715
Filing Date: 2013-06-04
Classification: C07K,C12N

Abstract:
1. A method for obtaining an organism in the genome of which a specific DNA fragment has been excised, comprising the steps of: a. providing an organism comprising, in its genome, a polynucleotide X comprising said DNA fragment, wherein said polynucleotide comprises at least a restriction site R, rare or absent in the genome of said plant, at its 5′ or 3′ extremity, b. providing, to cells of said organism, a restriction enzyme capable of cutting a DNA sequence at said restriction site(s) R, fused with a rat glucocorticoid receptor depicted in SEQ ID NO 1, and applying dexamethasone in order to generate a double strand break(s) at said site(s) thereby obtaining an organism wherein said DNA fragment has been excised after cutting at said restriction site(s) R and repair of the lesion generated by the double strand break(s).