Patent ID: 6670455
Filing Date: 2003-12-30
Classification: A61P,B01D,C12N

Abstract:
A process for the preparation of a protein wherein the protein is the pure form of the protease activating blood clotting factor VII, the pure form of a proenzyme for the protease, or a mixture of both the pure form of the protease and the pure form of the proenzyme, andwherein the protein is purified by a chromatography separation process, a fractional precipitation, or a combination of both a chromatography process and a fractional precipitation, and wherein during said chromatography separation process, the protein is absorbed on one of: a) calcium phosphate/hydroxyapatite b) a hydrophobic matrix; c) a chelate matrix d) a matrix on which heparin or a substance related to heparin is immobilized, or e) a matrix which is coated with an immobilized monoclonal or polyclonal antibody directed against the protein to be isolated, or F(ab) or F(ab)2 fragments of antibodies directed against the protein to be isolated.