Patent ID: 8163551
Filing Date: 2012-04-24
Classification: C07H,C07K,C12N

Abstract:
1. A method of making a modified antibody or antibody derivative having reduced core fucosylation, comprising: culturing a host cell in a culture medium comprising an effective amount of a fucose analog under suitable growth conditions, wherein the host cell expresses the antibody or antibody derivative having an Fc domain having at least one complex N-glycoside-linked sugar chain bound to the Fc domain through an N-acetylglucosamine of the reducing terminal of the sugar chain, and isolating the antibody or antibody derivative from the cells, wherein the fucose analog is selected from the group consisting of one of the following formulae (I) or (II): or a biologically acceptable salt or solvate thereof, wherein: each of formula (I) or (II) can be the alpha or beta anomer or the corresponding aldose form; each of R R wherein the antibody or antibody derivative has reduced core fucosylation compared to the antibody or antibody derivative from the host cell cultured in the absence of the fucose analog.