Patent ID: 7323317
Filing Date: 2008-01-29
Classification: C12Q,G01N

Abstract:
1. A kit for qualitatively or quantitatively detecting alkaline sphingomyelinase, said kit comprising the following components in separate containers: (a) an assay buffer at pH 8.9-9.1 which contains EDTA in an amount sufficient to inhibit neutral sphingomyelinase; (b) sphingomyelin; (c) bile salts; (d) a reaction buffer which contains EDTA and EGTA in amounts sufficient to inhibit neutral sphingomyelinase, and β-glycerophosphate and ATP in amounts sufficient to inhibit acid sphingomyelinase; (e) alkaline phosphatase; (f) choline oxidase; (g) horseradish peroxidase; and (h) fluorogenic probe for H