Patent ID: 8932825
Filing Date: 2015-01-13
Classification: A01K,C07K,C12N,C12P,C12Y

Abstract:
1. A method for producing a recombinant glycoprotein in a Pichia pastoris host cell that lacks OCH1 activity, the method comprising the steps of diminishing or depleting the activity of one or more enzymes-selected from the group consisting of: (a) an enzyme having dolichyl-P-Man:Man (b) an enzyme having dolichyl-P-Man:Man (c) an enzyme having dolichyl-P-Man:Man said host cell further expressing: (i) an α1,2-mannosidase catalytic domain fused to a targeting peptide that targets the endoplasmic reticulum (ER) or Golgi apparatus in the host cell, (ii) a GlcNAc transferase I (GnT I) catalytic domain fused to a targeting peptide that targets the ER or Golgi apparatus of the host cell, and (iii) the recombinant glycoprotein, wherein said method results in the production within the host cell of recombinant glycoproteins having N-glycans attached thereto comprising GlcNAcMan