Patent ID: 7449443
Filing Date: 2008-11-11
Classification: C07K,C12N,C12P

Abstract:
1. An artificial polypeptide with increased stability, relative to its corresponding wild type polypeptide, wherein said artificial polypeptide comprises a leucine zipper region wherein at least one amino acid residue is replaced with a hydrophobic non-natural amino acid, wherein said polypeptide is GCN4-pl or protein Al, wherein the hydrophobic non-natural amino acid is selected from the group consisting of: 5,5,5- trifluoroleucine, 5,5,5,5′,5′,5′-hexafluoroleucine, 2-amino-4-methyl-4-pentenoic acid, 2-amino-3,3,3 -trifluoro-methylpentanoic acid, 2-amino-3-methyl-5,5,5-tri-fluoropentanoic acid, 2-amino-3-methyl-4-pentenoic acid, trifluorovaline, and hexafluorovaline, and wherein the artificial polypeptide exhibits a higher ratio of folded to unfolded protein relative to its corresponding wild type polypeptide.