Patent ID: 6509454
Filing Date: 2003-01-21
Classification: C07K,G01N,Y10S

Abstract:
A method for separating a first polypeptide comprising an IgG moiety capable of binding Protein A from a second polypeptide lacking an IgG moiety capable of binding Protein A in a mixture comprising said first and second polypeptide in a liquid solution or suspension, said method comprising:contacting said mixture with a soluble ligand comprising Protein A, wherein the molecular weight of said ligand is smaller than the molecular weight of said first polypeptide and wherein said contacting results in the formation of a first polypeptide/ligand complex which complex differs in at least one physical property from said second polypeptide; separating said first polypeptide/ligand complex from said second polypeptide using a separation technique based on said differences in said physical property; and dissociating the components of the polypeptide/ligand complex.