Patent ID: 6472215
Filing Date: 2002-10-29
Classification: G01N,Y10T

Abstract:
A method of analyzing nucleated red blood cells in a blood sample comprising:(a) exposing a first aliquot of a blood sample to a first lysing reagent system to lyse red blood cells and to form a first sample mixture, (b) exposing a second aliquot of said blood sample to a second lysing reagent system to lyse red blood cells and to form a second sample mixture, (c) measuring said first sample mixture in a flow cell by a detection comprising a first direct current impedance measurement (DC1), radio frequency impedance measurement (RF), and light scatter measurement (LS), (d) measuring blood cell distributions of said second sample mixture by a second direct current impedance measurement (DC2), (e) analyzing blood cell distribution patterns obtained from measuring said first sample mixture, and differentiating nucleated red blood cells from other cell types, (f) analyzing blood cell distribution patterns obtained from measuring said second sample mixture, and differentiating nucleated red blood cells from other cell types, (g) performing a combined analysis on a profile obtained by combining results of analysis from step (e) and (f), said combined analysis further differentiating nucleated red blood cells from other cell types, and (h) reporting nucleated red blood cells in said blood sample.