Patent ID: 9056049
Filing Date: 2015-06-16
Classification: A23J,A23L,A23V,A61K

Abstract:
1. A method for obtaining a trypsin-inhibiting protein extract from a sweet potato comprising: a. grinding a cleaned and peeled sweet potato in a saw tooth grinding machine; b. homogenizing the ground sweet potato in a homogenizing machine to form sweet potato particles of uniform size, c. placing the sweet potato particles on ice to cool the sweet potato particles, and adding 1% to 5% acetic acid and 1% to 10% sodium chloride to the cooled sweet potato particles to obtain a crude extract; d. centrifuging the crude extract in a horizontal centrifuge to form a liquid extract and a precipitate; e. removing the precipitate from the centrifuged extract to obtain the liquid extract, wherein the liquid extract has trypsin-inhibitory activity; f. heating the obtained liquid extract to a temperature of 72° C. to 80° C.; g. placing the heated liquid extract on ice to cool it down to a temperature of 20° C. to 25° C.; h. filtering the cooled liquid extract through a filtration membrane to remove tiny particles from the liquid extract, and i. passing the filtered liquid extract obtained in step h through a microfiltration membrane to obtain said trypsin-inhibiting protein extract, wherein the protein molecules within the protein extract have a molecular weight of 20 KD to 200 KD.