Patent ID: 6117667
Filing Date: 2000-09-12
Classification: A61K,A61P,C12N,Y02A

Abstract:
A method for isolating a virus population adapted for growth on a cell line originating from the kidney of an African Green Monkey, wherein said cell line supports the growth of rotaviruses, astroviruses enteroviruses, polioviruses, respiratory viruses, and hepatitis A viruses, and wherein said cell line is substantially free of viable adventitious microbial agents, said method comprising the steps of:(i) inoculating said cell line with a sample containing an inoculum of a virus selected from the group consisting of rotaviruses, astroviruses, enteroviruses, polioviruses, respiratory viruses, and hepatitis A viruses;(ii) incubating the inoculated cell line to permit proliferation of said virus; and(iii) harvesting the adapted virus population thereby produced.