Patent ID: 7279283
Filing Date: 2007-10-09
Classification: G01N

Abstract:
1. An assay method for the determination of holo-Transcobalamin II (holo-TCII) in a body fluid sample, comprising: contacting a sample of a body fluid with an immobilized cobalamin or an immobilizable cobalamin which is bound to one member of a specific binding pair which selectively binds the apo-forms of TCII and haptocorrin (HC) in said sample over the holo-forms thereof, separating said selectively bound TCII from said sample or rendering said selectively bound TCII unable to bind to a specific binding ligand for TCII or holo-TCII, contacting the non-bound TCII with a specific binding ligand for TCII or holo-TCII to bind said holo-TCII to form a ligand bound fraction and a non-ligand bound fraction, and measuring the TCII content of said ligand bound fraction to determine the quantity of holo-TCII in the body sample being assayed.