Patent ID: 7651837
Filing Date: 2010-01-26
Classification: C12Q

Abstract:
1. A method for detecting and/or identifying microorganisms causative of infectious diseases, comprising the steps of: (a) taking phagocytes from a clinical specimen and adjusting cell density of the phagocytes to between 5×10 (b) immobilizing the phagocytes onto a solid support; (c) treating the phagocytes with lytic enzyme under the presence of protease inhibitor to increase phagocyte cell membrane permeability and bare DNA in the phagocytes; (d) in situ hybridizing the bared DNA with at least one DNA probe which can hybridize with DNA of at least one microorganism causative of an infectious disease under conditions wherein the hybridization of the at least one DNA probe is specific to the DNA of the at least one microorganism causative of an infectious disease; and (e) detecting and/or identifying the at least one microorganism causative of an infectious disease by detecting hybridization the of at least one probe to the bared DNA.