Patent ID: 8382972
Filing Date: 2013-02-26
Classification: G01N

Abstract:
1. A method for quantifying a chemical substance contained in a sample solution, the method comprising: a step (a) of preparing a sensor chip, wherein: a step (b) of supplying the sample solution to a surface of the sensor chip to cover the surface of the sensor chip with the sample solution, wherein the sample solution contains the chemical substance and an oxidation-reduction substance or contains the chemical substance modified with an oxidation-reduction substance; a step (c) of applying a potential to the first working electrode with potentiostat, and connecting the second working electrode to the stripping electrode to generate reactions represented by the following formulas (I) to (III) in surfaces of the first working electrode, the second working electrode, and the stripping electrode, respectively, the first working electrode: wherein, n represents an integer, and m represents a positive integer, the second working electrode: wherein, n represents an integer, and m represents a positive integer, and the stripping electrode: wherein, M represents a metallic element, and s represents a positive integer, and the metallic element is deposited on the surface of the stripping electrode; a step (d) of applying a potential to the stripping electrode in a condition where no potential is applied to either the first working electrode or the second working electrode to measure a current flowing through the stripping electrode; and a step (e) of calculating concentration of the oxidation-reduction substance as an oxidant to quantify the chemical substance on the basis of the calculated concentration.