Patent ID: 6107470
Filing Date: 2000-08-22
Classification: C07K

Abstract:
A compound of formula: ##STR12## wherein: R.sub.8 is H or an amine protecting group;R.sub.9 is H or alkyl having from 1 to about 12 carbon atoms or alkenyl having from 2 to about 12 carbon atoms;one of R.sub.4 and R.sub.5 is H and the other of R.sub.4 and R.sub.5 is a moiety of formula (II) ##STR13## wherein R.sub.10 is an amine protecting groupL is selected from the group consisting of hydrogen, hydroxy, (C.sub.1 -C.sub.4)alkanoyl, naturally occurring nucleobases, non-naturally occurring nucleobases, aromatic moieties, DNA intercalators, nucleobase-binding groups, and heterocyclic moieties, reporter ligands, wherein amino groups are, optionally, protected by amino protecting groups;A is a group of formula (IIa)-(IId): ##STR14## where: X is O, S, Se, NR.sup.3, CH.sub.2 or C(CH.sub.3).sub.2 ;Y is a single bond, O, S or NR.sup.4 where R.sup.4 is as described above;each r and s is zero or an integer from 1 to 5;each R.sup.1 and R.sup.2 is independently selected from the group consisting of hydrogen, (C.sub.1 -C.sub.4)alkyl which may be hydroxy- or alkoxy- or alkylthio-substituted, hydroxy, alkoxy, alkylthio, amino and halogen; andR.sup.3 is selected from the group consisting of hydrogen, (C.sub.1 -C.sub.4)alkyl, hydroxy- or alkoxy- or alkylthio-substituted (C.sub.1 -C.sub.4)alkyl, hydroxy, alkoxy, alkylthio and amino.