Patent ID: 8609415
Filing Date: 2013-12-17
Classification: A61F,A61L

Abstract:
1. A method for manufacturing a tissue-engineered construct, the method comprising: providing a cell-seeded scaffold in a bioreactor chamber, which bioreactor chamber being divided by the cell-seeded scaffold into a first compartment and a second compartment; subjecting the cell-seeded scaffold to a flow of nutrient medium within the bioreactor chamber for developing the cell-seeded scaffold into a tissue structure to develop the tissue construct; applying a dynamic pressure difference to the developing tissue structure by the flow of nutrient medium to induce a volumetric deformation of the developing tissue structure and a dynamic strain on the developing tissue structure; measuring the dynamic pressure difference; measuring the volumetric deformation of the developing tissue structure; determining a magnitude of at least one mechanical property of the developing tissue structure based on the measured volumetric deformation and based on the applied pressure difference while the tissue structure is within the bioreactor chamber; determining if the determined magnitude of at least one mechanical property exceeds a threshold value; continuing subjection of the developing tissue structure to the flow of nutrient medium if the determined magnitude of at least one mechanical property does not exceed the threshold value; and ending the subjection of the developing tissue structure to the flow of nutrient medium if the determined magnitude of at least one mechanical property exceeds the threshold value, thereby resulting in the tissue-engineered construct.