Patent ID: 6727409
Filing Date: 2004-04-27
Classification: A01N,C07K,C12N,C12R,Y02A

Abstract:
An isolated protein variant of the protein of SEQ ID NO:4, which variant has the same insecticidal characteristics as the protein of SEQ ID NO:4 or an insecticidally effective fragment thereof, and which variant comprises an amino acid sequence which is encoded by a DNA sequence hybridizing under stringent hybridization conditions to the DNA and wherein said stringent hybridization conditions are established as follows using the following consecutive steps:a) immobilizing DNA fragments on a filter; b) prehybridizing said filter for 1 to 2 hours at 42Â° C. in 50% formamide, 5Ã—SSPE, 2Ã—Denhardt's reagent and 0.1% SDS, or for 1 to 2 hours at 68Â° C. in 6Ã—SSC, 2Ã—Denhardt's reagent and 0.1% SDS; c) adding a hybridization probe which has been radiolabeled; d) incubating for 16 to 24 hours; e) washing said filter for 20 minutes at room temperature in 1Ã—SSC, 0.1% SDS; and f) washing said filter three times for 20 minutes each at 68Â° C. in 0.2Ã—SSC, 0.1% SDS; and g) autoradiographing said filter by exposing said filter for 24 to 48 hours to X-ray film at âˆ’70Â° C. with an intensifying screen.