Patent ID: 7323303
Filing Date: 2008-01-29
Classification: A61P,C12N,G01N

Abstract:
1. A method for detecting β-lactam antibiotics or β-lactamase inhibitors in a sample, comprising the steps of: (1) providing a modified β-lactamase for binding with said β-lactam antibiotics or β-lactamase inhibitors, wherein the modified β-lactamase is a wild-type β-lactamase having a residue at a position that corresponds to a position in the amino acid sequence of the (2) exposing the sample to said modified β-lactamase comprising a fluorescent indicator molecule, and (3) comparing the fluorescence emitted by the modified β-lactamase bound with said β-lactam antibiotics or β-lactamase inhibitors, with the fluorescence emitted by the β-lactamase from a control sample having no β-lactam antibiotics or β-lactamase inhibitor, whereby the presence of β-lactam antibiotics or β-lactamase inhibitors in a sample is detected.