Patent ID: 6300545
Filing Date: 2001-10-09
Classification: C07K,C12N

Abstract:
A method for mobilizing a viral replicon from a T-DNA, comprising:a) providing an Agrobacterium replicon having a T-DNA, wherein said T-DNA contains a viral replicon flanked by directly repeated target sites for a site-specific recombinase; andb) infecting a cell of a plant with an Agrobacterium carrying said Agrobacterium replicon under conditions that allow transfer of said T-DNA and expression of said recombinase in said cell;wherein said cell, said T-DNA, or said viral replicon contains a nucleotide sequence encoding said recombinase or an active fragment or variant thereof, and said nucleotide sequence is operably linked to a promoter that drives expression in said cell and wherein said fragment or variant retains site-specific recombination activity.