Patent ID: 8961766
Filing Date: 2015-02-24
Classification: B01L,G01N

Abstract:
1. A method for preparing a separation system for electrophoretically separating chemical and/or biological species, comprising: providing a capillary separation channel disposed on a substrate, comprising: introducing a measured portion of the second solution mixtures contained within two or more of the side channels reservoirs into the capillary separation channel until each of the solution mixtures contacts an adjacent solution mixture along a contact interface; mixing each of the plurality of first and second solution mixtures within the capillary separation channel by diffusion for a period of time; simultaneously exposing the mixed solution mixtures within the capillary separation channel to a quantity of UV light polymerizing the mixed solution mixture and thereby providing a length of polyacrylamide gel having a gradient in total acrylamide concentration; and providing a programmable high voltage power supply in electrical communication with the first and second ends of the capillary separation channel, the high voltage power supply providing an electric field within each of the capillary separation channels, wherein the electric field and the depth and width of the capillary separation channels cooperate to provide electrophoretic separation of a species complex introduced into the capillary separation channel in less than about 60 seconds.