Patent ID: 6780967
Filing Date: 2004-08-24
Classification: C07K,C12N,G01N

Abstract:
A process for the production of a metal-chelate labelled peptide, comprising at least one of the following procedures (1) and (2):(1) (a) synthesizing the peptide on a solid phase by attaching together a plurality of amino acids and/or amino acid derivatives; (b) providing protecting groups on any amino side groups of the amino acids and/or amino acid derivatives to which coupling with a luminescent metal chelate is to be avoided so that coupling of the luminescent metal chelate in the following step (c) may only proceed at the amino and/or carboxy terminus of the solid phase-bound peptide; and (c) after steps (a) and (b), coupling the luminescent metal chelate to the amino and/or carboxy terminus of the solid phase-bound peptide, and (2) synthesizing the peptide on a solid phase by attaching together a plurality of amino acids and at least one amino acid derivative which is coupled to a luminescent metal chelate, wherein the at least one amino acid derivative is introduced into at least one predetermined position on the peptide, wherein procedures (1) and/or (2) are conducted such that the peptide synthesized in the process comprises an immunologically reactive epitope region and a spacer region, and the luminescent metal chelate is coupled to the peptide in the spacer region.