Patent ID: 7807352
Filing Date: 2010-10-05
Classification: B01J,C07B,C07H,C12N,C12Q,C40B,G01N

Abstract:
1. A process for producing two or more copies of nucleic acids of interest in a library comprising the steps of: a) providing: wherein at least two of said discrete areas each comprises fixed or immobilized thereto: wherein the nucleotide sequences in said first set of nucleic acid primers are different from the nucleotide sequences in said second set of nucleic acid primers; wherein the nucleotide sequences of a first set of nucleic acid primers of a first discrete area and the nucleotide sequences of a first set of nucleic acid primers of a second discrete area differ from each other by at least one base; and wherein the nucleotide sequences of the second set of nucleic acid primers of a first discrete area and the nucleotide sequences of the second set of nucleic acid primers of a second discrete area are the same or identical; b) contacting a fixed or immobilized primer of said first set with a complementary sequence in said nucleic acid of interest; c) separating said fixed or immobilized extended first primer from said nucleic acid of interest template; d) extending said fixed or immobilized primer in the first set using said nucleic acid of interest as a template to generate a fixed or immobilized extended first primer; e) contacting a primer in said second set with a complementary sequence in said extended fixed or immobilized first primer; f) extending said fixed or immobilized primer in the second set using said fixed or immobilized extended first primer as a template to generate a fixed or immobilized extended second primer; g) separating said fixed or immobilized extended second primer from said extended first primer template; h) contacting a fixed or immobilized primer in the first set with a complementary sequence in said fixed or immobilized extended second primer; i) extending said fixed or immobilized primer in the first set using said fixed or immobilized extended second primer as a template to generate a fixed or immobilized extended first primer; j) separating said fixed or immobilized extended first primer from said extended second primer template; and k) repeating steps e) through j) above one or more times.