Patent ID: 9119828
Filing Date: 2015-09-01
Classification: A61K,C07K,C12N

Abstract:
1. A method of making monoclonal antibodies having specificity for both, the lipid and the lipid associated HIV protein antigen, comprising: a) obtaining liposomes having lipid epitopes to HIV-1 and modifying said liposomes by incorporating: (1) an adjuvant and (2) a protein or peptide epitope from HIV-1 virus; b) immunizing a mammal with said liposomes; c) producing said antibodies, wherein said antibodies have simultaneous recognition subsites to said lipid epitopes in said liposome and to said protein or peptide epitope of said HIV-1 virus; d) identifying said antibodies with simultaneous recognition subsites to said lipid epitopes in said liposome and to said protein or peptide epitope of said HIV-1 virus; e) isolating only said antibodies with simultaneous recognition subsites to said lipid epitopes in said liposome and to said protein or peptide epitope of said HIV-1 virus; and f) cloning said antibodies to obtain monoclonal dual-multi specific antibodies having dual or multi-specific antigen binding sites binding more than one antigenic epitope selected from lipid epitope and an HIB epitope.