Patent ID: 7255996
Filing Date: 2007-08-14
Classification: C12Q

Abstract:
1. A method of determining whether a test sample contains at least one of HIV-1 nucleic acid and HIV-2 nucleic acid, said method comprising the steps of: (a) combining said test sample with a pair of cross-reactive primers to form a reaction mixture, wherein said pair of cross-reactive primers is capable of co-amplifying HIV-1 and HIV-2 nucleic acid and comprises a first primer sequence that consists of SEQ ID NO:14, optionally comprising an upstream sequence that is not complementary to either HIV-1 or HIV-2 nucleic acid, and a second primer sequence that consists of SEQ ID NO:7, optionally comprising an upstream sequence that is not complementary to either HIV-1 or HIV-2 nucleic acid; (b) amplifying, in an in vitro nucleic acid amplification reaction, nucleic acid present in said reaction mixture of step (a); and (c) detecting in a hybridization reaction an HIV-1 amplicon, an HIV-2 amplicon, or a combination of both amplicons synthesized in step (b), thereby determining that said test sample contains at least one of HIV-1 nucleic acid and HIV-2 nucleic acid.