Patent ID: 7655422
Filing Date: 2010-02-02
Classification: C07K,C12Q,G01N,G16B

Abstract:
1. A method for identifying a compound that putatively modulates or elicits taste in a human subject comprising:(i) screening one or more compounds in a binding assay comprising contacting said one or more compounds with a human T1R2 polypeptide, wherein said T1R2 polypeptide is selected from the group consisting of: (a) a polypeptide which consists of or contains a polypeptide which is at least 90% identical to the entire extracellular or transmembrane regions of the human T1R2 polypeptide in SEQ ID NO: 21 and which polypeptide specifically binds to a ligand that specifically binds to the human T1R2 polypeptide in SEQ ID NO:21; (b) a human T1R2 polypeptide encoded by a nucleic acid sequence that specifically hybridizes to the complement of the hT1R2 nucleic acid sequence of SEQ ID NO: 23 under stringent hybridizations which are: conducting hybridization at 42 degrees C. in a solution comprising 50% formamide, 5XSSC, and 1% SDS, and washing at 65 degrees C. in a solution containing 0.2XSSC and 0.1% SDS, and which human T1R2 polypeptide specifically binds to a ligand that specifically binds to the human T1R2 polypeptide in SEQ ID NO:21; and (c) a human T1R2 polypeptide which has at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 21; and (ii) whereby a compound that putatively modulates or elicits taste is identified by its specific binding to a human T1R2 polypeptide according to (a), (b), (c) or its inhibition or enhancement of the specific binding of another compound to a T1R2 polypeptide according to (a), (b), or (c).