Patent ID: 8686218
Filing Date: 2014-04-01
Classification: C12N

Abstract:
1. A method of producing a filamentous fungus fruiting body to confer an altered phenotype, but a wild-type genotype comprising: seeding a lower nutrient substrate layer with a first filamentous fungal mycelial inoculant, so that said substrate becomes colonized with the mycelial inoculant, overlaying said lower nutrient substrate layer with an upper water-holding substrate layer that has been inoculated with a second transgenic filamentous fungal mycelial inoculant, wherein said second transgenic filamentous fungal mycelial inoculant incorporates a heterologous polynucleotide construct, and allowing fruiting bodies to form, wherein said formed fruiting bodies include the heterologous polynucleotide product from the second transgenic filamentous fungal mycelial inoculant.