Patent ID: 7252950
Filing Date: 2007-08-07
Classification: C12Q,G01N

Abstract:
1. A method of identifying a therapeutic lead compound that modulates activity of a cytoskeletal system, said method comprising: i) providing an assay mixture comprising a first component of said cytoskeletal system and a second component of said cytoskeletal system, wherein said cytoskeletal system is a microtubule system, and wherein said first component comprises a kinesin motor protein and said second component comprises a tubulin protein that specifically bind to each other; ii) contacting said assay mixture with a test compound to be screened for the ability to inhibit or enhance binding between said first component and said second component; iii) detecting a change in coupling between ATP hydrolysis and force generation; wherein said change indicates that said test compound modulates activity said cytoskeletal system.