Patent ID: 6207398
Filing Date: 2001-03-27
Classification: A61K,C07K,G01N

Abstract:
An enzyme-linked immunoassay for the measurement of cyclosporin A levels in whole blood samples comprising the steps of:(a) lysing red blood cells in a sample of whole blood containing cyclosporin A;(b) contacting the lysed whole blood sample with excess labeled anti-cyclosporin antibody, to form a labeled antibody-cyclosporin A complex;(c) separating unbound antibody from the complex by contacting the mixture formed in step (b) with a solid phase comprising a cyclosporin derivative having the structure: ##STR7##wherein X is selected from the group consisting of: ##STR8##wherein Y is selected from the group consisting of: ##STR9##wherein Z is selected from the group consisting of: ##STR10##wherein R.sub.1 and R.sub.2 are each a C.sub.1 -C.sub.8 alkyl group;wherein R.sub.3 is a bond or C.sub.1 -C.sub.8 alkyl group; andwherein m is 1-200,immobilized on a solid support; and(d) determining the amount of the label in the complex as a measure of cyclosporin A.