Patent ID: 9017687
Filing Date: 2015-04-28
Classification: A61K,C07K

Abstract:
1. A method for producing a low acidic species adalimumab composition, the method comprising: (a) contacting a first composition comprising adalimumab with a chromatography media, wherein the first composition comprises more than 10% total acidic species of adalimumab, wherein the acidic species of adalimumab have a net negative charge relative to adalimumab main species, wherein the acidic species of adalimumab comprise species selected from the group consisting of charge variants, structure variants, fragmentation variants, and any combinations thereof, wherein the acidic species of adalimumab do not include process-related impurities selected from the group consisting of host cell proteins, host cell nucleic acids, chromatographic materials and media components, and wherein the adalimumab binds to the chromatography media; (b) displacing the adalimumab bound to the chromatography media with a displacing buffer; and (c) collecting a second composition comprising the displaced adalimumab, wherein the second composition comprises less than 10% total acidic species of adalimumab, thereby producing a low acidic species adalimumab composition.