Patent ID: 8263750
Filing Date: 2012-09-11
Classification: B01D,C07K

Abstract:
1. A method for purifying an IgG1 or IgG2 antibody from a solution containing at least one contaminant by Protein A chromatography comprising: a) absorbing the IgG1 or IgG2 antibody to Protein A immobilized on a solid support; b) removing contaminants by washing the immobilized Protein A containing the absorbed IgG1 or IgG2 antibody with a wash buffer comprising 25 mM Tris, 1M to 3M urea, and an organic solvent selected from 10% (v/v) isopropanol or 10% (w/v) propylene glycol, and wherein said wash buffer has a pH of at least 9.0; and c) eluting the IgG1 or IgG2 antibody from the Protein A immobilized on the solid support.