Patent ID: 7247475
Filing Date: 2007-07-24
Classification: C07K,C12N

Abstract:
1. A method of optimizing the expression level of an antibody or a fragment thereof which comprises: (a) producing eukaryotic host cells containing and capable of expressing a first DNA sequence encoding at least an antibody heavy chain or an antigen binding portion thereof, said first DNA sequence being associated with a first heterologous selectable amplifiable marker gene, and a second DNA sequence encoding at least an antibody light chain or an antigen binding portion thereof, said second DNA sequence being associated with a second heterologous selectable amplifiable marker gene; (b) culturing said host cells in a suitable culture medium; (c) differentially amplifying said first and second DNA sequences with appropriate selective agents to allow maximized production of said antibody or fragment thereof (d) choosing a host cell that produces a desired amount of antibody.