Patent ID: 6537760
Filing Date: 2003-03-25
Classification: G01N,Y10S

Abstract:
Competitive receptor binding assay for the determination of TSH (thyroid-stimulating hormone) receptor autoantibodies in a biological sample, comprising reacting the sample, in a liquid reaction mixture, with(i) a labeled solubilized TSH receptor preparation, (ii) a labeled primary competitor for at least a first portion of the TSH receptor autoantibodies in the sample, and (iii) an agent for immobilizing complexes formed between said TSH receptor and components of the reaction mixture, and detecting the amount of the primary competitor immobilized in the TSH receptor complexes, wherein the presence and/or amount of the TSH receptor autoantibodies are determined on the basis of the amount of the primary competitor immobilized in the TSH receptor complexes, and wherein the agent for immobilizing the complexes is at least one specific monoclonal or polyclonal antibody bound to a solid phase that specifically recognizes a defined partial peptide sequence on the TSH receptor selected from the group consisting of amino acids 20-39, (SEQ ID NO:1) 32-54, (SEQ ID NO:2) 287-301 SEQ ID NO:3, 361-381, SEQ ID NO:4 and 739-758 SEQ ID NO:5 of the amino acid sequence of the complete TSH receptor including the signal peptide without impairing the binding capability of the TSH receptor with respect to the TSH receptor autoantibodies or with respect to the primary competitor.