Patent ID: 6544738
Filing Date: 2003-04-08
Classification: C12Q

Abstract:
An isolated nucleic acid composition comprising:A) a first oligonucleotide covalently linked to a removable tag, wherein the removable tag by a first photocleavable linker, is an amino acid, a peptide, a polymer, biotin, digoxygenin, a fluorescent dye or a dye impregnated bead, wherein the tag is detectable by a method selected from the group consisting of absorption spectra, antibody binding and optical sorting, and wherein at least a portion of the first oligonucleotide is complementary to a first region of a transcribed gene, and B) a second oligonucleotide covalently linked to a selector tag by a second photocleavable linker, wherein at least a portion of the second oligonucleotide sequence is complementary to a second region of the transcribed gene, wherein the removable tag and the selector tag are distinguishable, wherein the first and the second photocleavable linker are cleavable at different wavelength and wherein the first oligonucleotide and the second oligonucleotide can be used as a primer pair to amplify a target region of the transcribed gene.