Patent ID: 9085773
Filing Date: 2015-07-21
Classification: C12N

Abstract:
1. A method for identifying a nucleic acid ligand of a target molecule from a candidate mixture of nucleic acids, the method comprising: contacting at least one target molecule with a solution comprising a candidate mixture of nucleic acids, wherein the nucleic acids have different affinities for the target molecule; and separating in a single step nucleic acids that bind the target molecule with greatest affinity from nucleic acids that bind the target molecule with a lesser affinity and nucleic acids that do not bind the target molecule, wherein separating is by a liquid chromatography or gel technique using a gradient profile that comprises a linearly increasing concentration of the target molecule and the end portion of the gradient profile comprises a linearly increasing concentration of the target molecule and a linearly increasing concentration of a chaotropic agent, and wherein the separating step is performed only a single time, thereby identifying the nucleic acid ligand to the target molecule.