Patent ID: 8486700
Filing Date: 2013-07-16
Classification: A61K,C12N

Abstract:
1. A medium for differentiating adipose tissue derived stromal cells into hematopoietic supporting stromal cells comprising a chemically defined medium having or supplemented with the following components: (i) 0% to 20% fetal bovine serum; (ii) antibiotic; (iii) interleukins; (iv) about 5 pg/ml to 1 ng/ml stem cell factor; (v) about 5 pg/ml to 1 ng/ml flt-3ligand; (vi) about 5 pg/ml to 1 ng/ml macrophage-colony stimulating factor; (vii) about 5 pg/ml to 1 ng/ml granulocyte-monocyte colony stimulating factor; (viii) about 5 units/ml to 1000 units/ml erythropoietin; (ix) about 5 pg/ml to 1 ng/ml thrombopoietin; (x) about 5 pg/ml to 1 ng/ml osteoprotegerin ligand; (xi) about 1 nM to 100 nM dexamethasone; (xii) about 1 nM to 100 nM hydrocortisone; (xiii) about 1 nM to 100 nM 1,25 dihydroxy vitamin D 3 ; and (xiv) about 10 uM to 100 uM 2-mercaptoethanol and wherein components (ii)-(xiv) are present.