Patent ID: 7875276
Filing Date: 2011-01-25
Classification: A61F,A61K,C12N

Abstract:
1. A method for repairing a damaged urinary tract tissue of a subject, comprising the steps of: isolating and culturing at least one bone marrow stromal cell from a tissue specimen of a subject; isolating and culturing at least one cell type selected from the group consisting of smooth muscle cells and urothelial cells from the tissue specimen of the subject; providing a segment of small intestinal submucosa having a mucosal surface and a serosal surface; seeding the at least one bone marrow stromal cell on a surface of the segment of small intestinal submucosa; seeding the at least one cell type selected from the group consisting of smooth muscle cells and urothelial cells on a surface of the segment of small intestinal submucosa; allowing the segment of small intestinal submucosa having the cell types seeded thereon to mature in culture such that the at least one bone marrow stromal cell differentiates into a smooth muscle-like cell and both cell types exhibit three dimensional growth and matrix penetrance; and contacting the damaged urinary tract tissue with the seeded segment of small intestinal submucosa under conditions such that growth of the urinary tract tissue occurs and the damaged urinary tract tissue is repaired, thereby restoring urological function.