Patent ID: 8940546
Filing Date: 2015-01-27
Classification: G01N,Y10T

Abstract:
1. A method of measuring the relative abundances of peptide analytes by mass spectrometry, comprising: preparing a plurality of peptide-containing samples; reacting each sample with an isobaric labeling reagent selected from an isobaric labeling reagent set to create differentially labeled peptides, the isobaric labeling reagent set consisting of a plurality of different isobaric labeling reagents wherein: wherein each sample is reacted with a unique one of the isobaric labeling reagents, such that labeled peptides in each sample have a reporter group mass that is different from the reporter group mass of the labeled peptides in each of the other samples, and wherein at least two of the samples are reacted with corresponding isobaric labeling reagents of the first collection; combining the samples; ionizing the combined samples to generate labeled peptide ions; fragmenting the labeled peptide ions to produce reporter ions, the reporter ions having a mass-to-charge ratio uniquely characterizing one of the samples; and measuring the abundances of the reporter ions at each characteristic mass-to-charge ratio using a mass analyzer.