Patent ID: 6207432
Filing Date: 2001-03-27
Classification: C07K,C12N,G01N

Abstract:
A substantially pure tyrosylprotein sulfotransferase produced by the process comprising:providing a host cell transformed or transfected with a vector comprising a polynucleotide encoding a tyrosylprotein sulfotransferase, wherein the polynucleotide is selected from the group consisting of:(a) a polynucleotide which hybridizes to a nucleic acid sequence comprising nucleotides 82-1194 of SEQ ID. NO:2, nucleotides 211-1323 of SEQ ID NO:4, nucleotides 198-1331 of SEQ ID NO:6, nucleotides 157-1287 of SEQ ID NO:8, nucleotides 66-1208 of SEQ ID NO:10; or nucleotides 1-1077 of SEQ ID NO:12; and(b) a polynucleotide which hybridizes to a nucleic acid sequence comprising nucleotides 289-1086 of SEQ ID NO:2, nucleotides 418-1215 of SEQ ID NO:4, nucleotides 402-1199 of SEQ ID NO:6, nucleotides 358-1155 of SEQ ID NO:8, nucleotides 273-1064 of SEQ ID NO:10, or nucleotides 256-1029 of SEQ ID NO:12; andwherein the polynucleotides of (a)and (b) hybridize under hybridization condition comprising incubation at 68.degree. C. overnight followed by washing twice with two times SSC, 0.1% SDS at 22.degree. C., and washing twice with 0.1 times SSC, 0.1% SDS at 50.degree. C.;culturing the host cell in a manner which causes the expression of the tyrosylprotein sulfotransferase; andpurifying the tyrosylprotein sulfotransferase from the cultured host cell.