Patent ID: 6767709
Filing Date: 2004-07-27
Classification: G01N,Y10S

Abstract:
A method of immunologically measuring the human medullasin content in blood comprising the following steps (a) and (b):(a) breaking up the leukocytes in a blood sample by contacting said blood sample with the following aqueous liquid (i) or an aqueous liquid mixture of (i) and (ii) (i) an aqueous liquid, which contains 0.05 mole % or more or 0.005 mole % or less of a solute, having an osmotic pressure of 250 mOsm/kg.H2O or less or an aqueous liquid having an osmotic pressure of 310 mOsm/kg.H2O or more; (ii) an aqueous liquid comprising a hemolysate; and (b) immunologically determining content of human medullasin in said blood sample by a method comprising contacting the blood sample containing said human medullasin released from the leukocytes broken up in said step (a) with an anti-human medullasin monoclonal antibody immobilized to an insoluble carrier in the presence of a labeled anti-human medullasin monoclonal antibody to form a sandwich complex and to capture the human medullasin on a labeled immuno complex by an antigen-antibody reaction, and then determining the amount of activity of the label material in said complex.