Patent ID: 6924128
Filing Date: 2005-08-02
Classification: A61K,C07K,C12N

Abstract:
1. A method of producing a mammalian cell for packaging of a recombinant AAV (rAAV) vector, said method comprising: replicating a mammalian cell to produce a population of cells; wherein the mammalian cell comprises a stably integrated AAV cap gene operably linked to AAV p40 promoter, and a stably integrated AAV rep gene operably linked to a helper virus-inducible heterologous promoter, wherein (a) the AAV cap gene and the AAV rep gene are stably integrated into the mammalian cell's genome; (b) p5 promoter function has been replaced by the helper virus-inducible heterologous promoter; (c) said mammalian cell was prepared by introducing a plasmid comprising both AAV rep and AAV cap arranged as in the AAV genome into the mammalian cell; and (d) upon introduction of a helper virus into the population of the cells, said cells exhibit helper virus-inducible expression of said stably integrated AAV rep gene.