Patent ID: 6284493
Filing Date: 2001-09-04
Classification: C07K,C12N,C12P

Abstract:
A method, for the glycosyltransferase-catalyzed preparation of a preselected saccharide composition having a defined sequence of saccharide subunits by serially bonding saccharide units to an acceptor moiety comprising:(a) contacting said acceptor moiety with a first saccharide unit in the presence of a first cell surface genetically engineered to express a first cell surface-bound glycosyltransferase specific for catalyzing the linkage of said acceptor moiety with said first saccharide unit to form a first saccharide composition having a defined sequence of saccharide subunits; and(b) contacting said first saccharide composition with a second saccharide unit in the presence of a second cell surface genetically engineered to express a second cell surface-bound glycosyltransferase specific for catalyzing the linkage of said first saccharide composition with said second saccharide unit, wherein said second saccharide unit is different from said first saccharide unit, to form a preselected serially bound second saccharide composition having a defined sequence of saccharide subunits:wherein the first and second cell surface-bound glycosyltransferases are each selected from the group consisting of a galactosyltransferase, a sialyltransferase, a fucosyltransferase, an N-acetylgalactosaminyltransferase, an N-acetylglucosaminyltransferase, and a glucuronyltransferase.