Patent ID: 6692750
Filing Date: 2004-02-17
Classification: A61P,C12N

Abstract:
A method for producing a recombinant alphavirus comprising a recombinant alphavirus RNA genome surrounded by a wild-type alphavirus coat;the RNA genome comprising a self-replicative and transcription competent recombinant alphavirus RNA molecule comprising at least part of an alphavirus RNA genome and heterologous RNA encoding a substance having biological activity located downstream of and operatively linked to an alphavirus base sequence having translation enhancing activity, the translation enhancing base sequence being comprised of a complete alphavirus capsid gene or a portion thereof; comprising: i) introducing the recombinant alphavirus RNA genome and a helper RNA encoding alphavirus structural proteins and comprising coding sequences for the alphavirus structural proteins, cis acting replication signals but no encapsidation signals into cells; ii) producing infectious recombinant alphavirus particles by incubating the cells in a medium; and iii) collecting the medium containing infectious recombinant alphavirus particles.