Patent ID: 6753152
Filing Date: 2004-06-22
Classification: C07K

Abstract:
A method for in vitro immunodetection of TAG-72-expressing cancer cells comprising a step of contacting the cancer cells with a diagnostically effective amount of a composition suitable for in vitro immunodetection of cancer, said composition containing either:A) a diagnostically effective amount of a humanized antibody which specifically binds TAG-72 and which comprises: 1) at least one NEWM-grafted humanized heavy chain variable region (VH) having the amino acid sequence of any one of SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6; and 2) at least one REI-grafted humanized light chain variable region (VL) having the amino acid sequence of SEQ ID NO:9; or B) a diagnostically effective amount of a humanized antibody fragment which specifically binds TAG-72, said fragment being a fragment of the humanized antibody of Part A); and said method comprising a step of detecting the resulting specifically bound humanized antibody, specifically bound humanized antibody fragment, or specifically bound cancer cell.