Patent ID: 7223854
Filing Date: 2007-05-29
Classification: A61K,A61P,C07K,C12N,G01N

Abstract:
1. A diagnostic kit comprising: (a) a first primer comprising a polynucleotide having a sequence of 10 to 50 nucleotides in length which hybridizes to a genomic polynucleic acid from a North American strain of PRRSV, wherein said North American strain is ISU-12 (VR 2385), at a temperature of 25 to 75° C., (b) a second primer comprising a polynucleotide having a sequence of 10 to 50 nucleotides in length which hybridizes to said genomic polynucleic acid from said North American strain of PRRSV at a temperature of 25 to 75° C. and being downstream from the sequence to which said first primer hybridizes, and (c) a reagent which enables detection of an amplified polynucleic acid, wherein said first and second primers selectively hybridize to said North American strain and at least one of said first and second primers does not hybridize to a European PRRSV strain, wherein said European strain is Lelystad, resulting in amplification of said North American strain but no amplification of said European strain.