Patent ID: 6399322
Filing Date: 2002-06-04
Classification: A01K,A61K,A61P,C07K,G01N

Abstract:
A method for identifying a protein from Th2 cells that interacts with a maf family protein, comprising:a) providing a population of indicator cells, which population of indicator cells comprises: i) a maf family protein lacking a transcriptional activation domain; ii) a cDNA library prepared from Th2 cells; and iii) a reporter gene operably linked to a transcriptional regulatory sequence to which the maf family protein binds; wherein expression of the reporter gene is sensitive to interactions between the maf family protein, a protein encoded by the cDNA library and the transcriptional regulatory sequence; and b) selecting indicator cells within the population in which reporter gene expression is stimulated to thereby identify a protein from Th2 cells that interacts with a maf family protein; and c) wherein the maf family protein is a c-Maf protein.