Patent ID: 7303872
Filing Date: 2007-12-04
Classification: C12N

Abstract:
1. A method for making DNA sequences of pre-selected defined sequence using a microarray synthesis process that makes occasional errors, the method comprising the steps of (a) making a microarray of single stranded DNA probes, the probes constructed so that each probe has a complementary portion that is partially complementary to another probe on the microarray and further constructed so that for each set of probes a complete complementary set of probes is constructed, a minority of the probes in the microarray being erroneously made with a sequence not the defined sequence; (b) releasing the single stranded DNA probes from the microarray; (c) cooling the single stranded probes so that DNA duplexes are formed, the duplexes formed of probes hybridized to their complete complementary probe having the defined sequence while duplexes formed with single stranded DNA probes not of the defined sequence having an irregularity in its topographical shape; (d) exposing the DNA duplexes to a DNA binding agent which will selectively bind to a DNA duplex which has an irregularity in its topographical shape; (e) separating out the DNA duplexes to which the DNA binding agent bound; (f) denaturing the DNA duplexes to release the single stranded DNA probes from the DNA duplexes; (g) cooling the DNA duplexes under conditions which favor at least some of the single stranded DNA probes binding to the probes to which they are only partially complementary to form DNA complexes which are double stranded in at least some part; and (h) extending the DNA complexes thus made to add a second DNA strand to remaining single stranded parts of the DNA complexes.