Patent ID: 6225121
Filing Date: 2001-05-01
Classification: C07K,C12N

Abstract:
A method for integrating a nucleic acid sequence of interest into the chromosome of a cell, comprising the steps of:a) providing an isolated transposable element having a nucleic sequence which hybridizes to the DNA sequence of SEQ ID NO:1 or SEQ ID NO:4 in a buffered solution of 0.9 M NaCl, at a temperature of 55.degree. C., the isolated transposable element being modified to include the nucleic acid sequence of interest flanked by the inverted terminal repeats of the isolated transposable clement; andb) producing a sample by introducing the isolated transposable element of step a) into the cell in the presence of:i) a transposase protein encoded by a nucleic acid sequence which hybridizes to the DNA sequence of SEQ ID NO: 1 or SEQ ID NO:4 in a buffered solution of 0.9 M NaCl, at a temperature of 55.degree. C.; orii) an expressible nucleic acid sequence encoding a transposase protein, the expressible nucleic acid sequence being characterized by the ability to hybridize to the DNA sequence of SEQ ID NO:1 or SEQ ID NO:4 in a buffered solution of 0.9 M NaCl, at a temperature of 55.degree. C.