Patent ID: 9182334
Filing Date: 2015-11-10
Classification: C12Q,G01N

Abstract:
1. A method for measuring a property of a binding interaction between a capture agent and a binding partner for said capture agent, comprising: a) contacting a population of particles that are linked to a capture agent with a substrate comprising a binding partner to produce capture agent/binding partner complexes, wherein said population of particles comprises first particles that are bound to a single molecule of said capture agent and second particles that are bound to two molecules of said capture agent; b) applying a force to the particles, wherein said force is in a direction that separates said particles from said substrate; c) separately measuring: d) calculating the off rates of said first particles and said second particles as a function of the applied force; e) calculating the off rate for said first particles in the absence of applied force from the dependence of off rates on the applied force; and f) calculating the on rates for binding said first particles and said second particles, using the respective off rates calculated in d) and e).