Patent ID: 8310672
Filing Date: 2012-11-13
Classification: G01J,G01N

Abstract:
1. A method for generating and for detecting a Raman spectrum of a medium to be analyzed having the following procedure steps: exposing the medium to be analyzed to excitation radiation, wherein the medium to be analyzed is exposed in a time-shifted manner to a first excitation radiation having a first wavelength and to a second excitation radiation having a second wavelength, wherein the first wavelength differs from the second wavelength, spectral analysis of the first excitation radiation scattered by the medium to be analyzed, wherein a first spectrum that assigns one intensity value each to a plurality of wavelengths is detected from the scattered first excitation radiation, spectral analysis of the second excitation radiation scattered by the medium to be analyzed, wherein a second spectrum that assigns one intensity value each to a plurality of wavelengths is detected from the scattered second excitation radiation, calculating a Raman spectrum of the medium to be analyzed from the first spectrum and the second spectrum, wherein the first spectrum and the second spectrum are normalized to each other with respect to their intensity values, and subsequently: a) a first difference spectrum is calculated from the difference between the first spectrum and the second spectrum, b) a second difference spectrum is calculated from the difference between the second spectrum and the first spectrum, c) the first difference spectrum is converted into a first transformation spectrum, wherein one intensity value each is assigned to a plurality of wavelengths, and the intensity value of a wavelength of the first transformation spectrum is calculated by adding a first intensity value of a first wavelength of the first difference spectrum and the amount of a second intensity value of a second wavelength of the first difference spectrum, wherein the first wavelength of the first difference spectrum is greater than the wavelength of the first transformation spectrum by a predetermined transformation parameter and the second wavelength of the first difference spectrum is smaller than the wavelength of the first transformation spectrum by the transformation parameter, d) the second difference spectrum is converted into a second transformation spectrum, wherein one intensity value each is assigned to a plurality of wavelengths, and the intensity value of a wavelength of the second transformation spectrum is calculated by adding the amount of a first intensity value of a first wavelength of the second difference spectrum and a second intensity value of a second wavelength of the second difference spectrum, wherein the first wavelength of the second difference spectrum is greater than the wavelength of the second transformation spectrum by the transformation parameter and the second wavelength of the second difference spectrum is smaller than the wavelength of the second transformation spectrum by the transformation parameter, and e) the Raman spectrum is calculated by adding the first transformation spectrum and the second transformation spectrum.