Patent ID: 7022500
Filing Date: 2006-04-04
Classification: A61K,A61P,C07K

Abstract:
1. A humanized immunoglobulin having complementarity determining regions (CDRs) from a donor immunoglobulin and heavy and light chain variable region frameworks from acceptor immunoglobulin heavy and light chains, which humanized immunoglobulin specifically binds to an antigen with an affinity constant within about four-fold that of the donor immunoglobulin, wherein said humanized immunoglobulin comprises at least three amino acids from the donor immunoglobulin heavy chain framework outside the Kabat and Chothia CDRs that replace the corresponding amino acids in the acceptor immunoglobulin heavy chain framework, wherein each of these at least three donor amino acids: (I) is immediately adjacent to one of the CDRs, or (II) is capable of interacting with the CDRs, or (III) is typical at its position for human immunoglobulin sequences, and the replaced amino acid is rare at its position for human immunoglobulin sequences.