Patent ID: 8389218
Filing Date: 2013-03-05
Classification: C12Q

Abstract:
1. A method comprising: a) contacting a sample comprising double-stranded genomic DNA with a site-specific nicking endonuclease that recognizes a nucleotide sequence that comprises a single nucleotide polymorphism (SNP) in said double stranded genomic DNA to provide a contacted genomic sample, wherein said endonuclease nicks said genomic DNA at a nick site that is proximal to said SNP only if a first allele of said SNP is present; b) denaturing said contacted genomic sample to provide a denatured genomic sample; c) contacting under hybridization conditions said denatured genomic sample with an array of probes comprising a first probe and a second probe, wherein: d) comparing the amount of hybridization to said first probe to the amount of hybridization to said second probe, wherein decreased binding of said denatured genomic samples to said first probe relative to said second probe indicates that said first allele of said SNP is present.