Patent ID: 8765412
Filing Date: 2014-07-01
Classification: C07K

Abstract:
1. A method of preparing a heteromultimeric multispecific antibody comprising the steps of: (i) culturing a host cell comprising recombinant nucleic acids which encode a first heavy chain polypeptide, a second heavy chain polypeptide, and a common light chain polypeptide, such that the polypeptides are expressed and form a heteromultimeric multispecific antibody, wherein the first heavy chain polypeptide and the second heavy chain polypeptide each comprise a heavy chain constant domain and a heavy chain variable domain, wherein said first heavy chain polypeptide and said common light chain polypeptide form a binding domain that binds a first antigen, and wherein said second heavy chain polypeptide and said common light chain polypeptide form a binding domain that binds a different antigen, wherein the common light chain is the only light chain expressed in the host cell, and wherein said first heavy chain polypeptide and said second heavy chain polypeptide multimerize to form a heteromultimeric multispecific antibody; and (ii) recovering the multispecific antibody from the host cell culture.