Patent ID: 6680060
Filing Date: 2004-01-20
Classification: A61K,A61P,C07K,C12N,Y02A

Abstract:
An isolated DNA molecule encoding a hepatitis A virus adapted to growth in MRC-5 cells, said molecule having a nucleotide sequence which corresponds to the cDNA sequence of HAV HM-175 Pass 35 (SEQ ID NO: 3) except for nucleotide positions 591 and 687 which have guanines as bases, nucleotide position 646 which has an adenine as a base and nucleotide position 669 which has a thymine as a base, and optionally at least one nucleotide selected from the group consisting of:(a) T at position 2750; (b) A at position 3027; (c) A at position 3196; (d) G at position 3934; (e) I at position 4418; (f) G at position 4563; (g) T at position 4643; (h) G at position 5145; (i) T at position 5745; (j) C at position 6908; (k) T at position 7032; (l) T at position 7255; wherein the nucleotide numbers shown above are those assigned to positions of the wild-type HM-175 sequence.