Patent ID: 6746870
Filing Date: 2004-06-08
Classification: C12N

Abstract:
A method for obtaining site-specific recombination in a eukaryotic cell, the method comprising:providing a eukaryotic cell that comprises an attB recombination site and an attP recombination site, which attP recombination site can serve as a substrate for recombination with the attB recombination site; contacting the attB and the attP recombination sites with a prokaryotic recombinase polypeptide, resulting in recombination between the attB and attP recombination sites; thereby forming an attL site and an attR site; wherein the recombinase polypeptide can mediate site-specific recombination between the attB and attP recombination sites, but cannot mediate recombination between the attL site and the attR site formed from recombination between the attB recombination site and the attP recombination site in the absence of an additional factor that is not present in the eukaryotic cell; and wherein the eukaryotic cell comprises a polynucleotide that encodes the recombinase polypeptide.