Patent ID: 8748182
Filing Date: 2014-06-10
Classification: A61P,C07K,C12N,G01N

Abstract:
1. A method of producing a mutant GPCR with increased stability in a particular conformation under denaturing conditions relative to its parent GPCR in the same particular conformation under denaturing conditions, the method comprising: (a) identifying in the amino acid sequence of one or more mutants of a first parent GPCR with increased stability in a particular conformation under denaturing conditions relative to the first parent GPCR in the same particular conformation under denaturing conditions, the position or positions at which the one or more mutants have at least one different amino acid residue compared to the first parent GPCR, and (b) making one or more mutations in the amino acid sequence that defines a second GPCR at the corresponding position or positions, to provide one or more mutants of a second parent GPCR with increased stability in a particular conformation under denaturing conditions relative to the second parent GPCR in the same particular conformation under denaturing conditions, or