Patent ID: 8101368
Filing Date: 2012-01-24
Classification: C12Q,G01N

Abstract:
1. A method to determine DNA content and antigen content per cell in a sample of cells, comprising: (a) fixing and permeabilizing of the cells; (b) incubating the sample of cells with an intercalator tagged with a first element under conditions appropriate to incorporate the intercalator into the DNA within the cells of the sample; (c) removing unbound intercalator from the cells; (d) incubating antigen specific affinity products labeled with a second element tag with the cells; (e) removing unbound affinity products from the cells; (f) simultaneously measuring the intercalator tagged with a first element and the affinity product labeled with the second element tag by elemental analysis using an Inductively Coupled Plasma Mass Spectrometer; and (g) calculating the DNA content and antigen content per cell.