Patent ID: 8338166
Filing Date: 2012-12-25
Classification: B01L,C12Q,G01N

Abstract:
1. An apparatus for identifying the known and unknown pathogenic or non-pathogenic organisms in a sample wherein the organisms include nucleic acids, comprising: a microfluidic flow channel, an emulsifier source that directs an emulsifier into said microfluidic flow channel, a droplet generator that directs the sample into said emulsifier and creates discrete droplets from the sample wherein said droplets constitute sub-nanoliter volume reactors containing the organisms; a lysis device for performing lysis of the organisms to release the nucleic acids; an amplifier for amplifying the nucleic acids; a fractionater that releases the nucleic acids from said droplets; and a parallel analyzer for identifying all of the known and unknown pathogenic or non-pathogenic organisms in the sample.