Patent ID: 7479370
Filing Date: 2009-01-20
Classification: C12Q

Abstract:
1. A method of diagnosing an individual human as likely of having lymphoblastic leukemia (ALL) comprising the steps of: a) obtaining a sample of bone marrow or blood comprising lymphocytes from the individual, and b) detecting by fluorescence in situ hybridization (FISH) whether part of the 13q14 region of chromosome 13 present in bacterial artificial chromosome (BAC) RP11-147H23 is deleted in the lymphocytes, wherein the FISH analysis is performed using a test probe and a control probe, wherein the test probe is BAC RP11-147H23, and wherein the control probe is complementary to a sequence of chromosome 13 outside the region of chromosome 13 present in BAC RP11-147H23; wherein, in the FISH analysis, two pairs of co-localized signals from the test probe and control probe in the lymphocytes is indicative of the absence of a deletion, and wherein a split signal from the test probe and one pair of co-localized signals is indicative of the presence of a deletion; wherein a deletion detected in step b) is indicative of a likelihood of the individual as having ALL.