Patent ID: 6509152
Filing Date: 2003-01-21
Classification: A01K,C07K,C12N,C12Q,G01N

Abstract:
A method for determining the presence of a test agent which induces complex formation between a RAPT1 protein and an FK506-binding protein (FKBP), comprising:(i) providing a host cell containing (a) a first chimeric gene encoding a first hybrid protein comprising a rapamycin-binding domain of an FKBP; (b) a second chimeric gene encoding a second hybrid protein comprising a domain which binds to an FKBP:rapamycin complex and is at least 80% identical to a sequence selected from at least one of SEQ ID NO: 2, 12, or 14; wherein one of the first and second hybrid proteins contains a DNA-binding domain and the other hybrid protein contains a transcriptional activation domain; and (c) a reporter gene, the transcription of which is induced by the presence of rapamycin, wherein said reporter gene is operably linked to a transcriptional regulatory site which binds to said DNA-binding domain; (ii) contacting the host cell with a test agent; and (iii) determining whether transcription of the reporter gene is dependent on addition of the test agent.