Patent ID: 6632611
Filing Date: 2003-10-14
Classification: C12Q

Abstract:
A method of obtaining a population of target sequences, wherein each of said target sequences comprises a region of interest, comprising:fragmenting a sample population of nucleic acids to produce fragments; ligating an adaptor sequence to the 5â€² ends of said fragments; combining said fragments with a collection of selection probes under conditions allowing for hybridization of complementary selection probes and target sequences to form probe-target complexes, each of said selection probes comprising a region that is complementary to a target sequence in said population of target sequences and further comprising a unique sequence at the 5â€² end; extending the target sequences in the probe-target complexes using the selection probe in the probe-target complex as a template and thereby incorporating the complement of the unique sequence from the 5â€² end of the selection probes into the 3â€² end of the extended target sequences; and amplifying the extended target sequences using a first primer that is complementary to the extended region at the 3â€² end of the extended target sequences and a second primer that is complementary to the adaptor region at the 5â€² end of the extended target sequences.