Patent ID: 6555527
Filing Date: 2003-04-29
Classification: A61K,A61P

Abstract:
A polysaccharide composition having hematopoietic, myeloprotecting, antitumor immune cell generating, and radiosensitizing activities extracted from the roots of Panax ginseng comprising 6 fractions of polysaccharide having molecular weights of 1,800,000Ëœ2,200,000, 1,350,000Ëœ1,650,000, 620,000Ëœ780,000, 105,000Ëœ130,000, 23,000Ëœ27,000, 5,000Ëœ6,000 dalton, with weight ratios of 11.4Ëœ13.4:3.6Ëœ4.2:4.5Ëœ5.1:0.7Ëœ0.9:40.1Ëœ48.1:31.0Ëœ37.0, respectively, consisting of &agr;(1â†’6) linked D-glucopyranose units with partly &agr;(1â†’3) linked branches,wherein said 6 fractions of polysaccharide are prepared by the steps of: i) obtaining a residue by extracting 1 wt part of ginseng root with 2Ëœ4 wt part of methanol; ii) extracting and obtaining a water soluble fraction from the residue in step i) using 3Ëœ5 wt part of distilled water; iii) freeze-drying the obtained water soluble fraction in step ii); iv) obtaining an inner fraction using the dialysis membrane after obtaining an insoluble fraction from the freeze-dried fraction in step iii); v) obtaining the 6 fractions by sephacryl S-500 gel chromatography; and vi) passing the polysaccharide composition obtained in step v) through DEAE-sephadex A-50 chromatography, and said 6 fractions of polysaccharide comprises more than 98.5% amount of carbohydrates moiety and less than 1.0% amount of proteins moiety by quantitative determination.