Patent ID: 7935513
Filing Date: 2011-05-03
Classification: C07K,C12N,C12P

Abstract:
1. A yeast or filamentous fungus host cell genetically engineered so that it does not display a 1,6 mannosyltransferase activity and produces a recombinant glycoprotein, comprising a nucleic acid encoding an alpha-1,2 mannosidase enzyme, the enzyme comprising: (i) an alpha-1,2 mannosidase catalytic domain; fused to (ii) a cellular targeting signal peptide not normally associated with the catalytic domain and selected to target the enzyme to the ER or Golgi apparatus of the host cell where the enzyme functions optimally; wherein the host cell produces the recombinant glycoprotein in which greater than 30 mole percent of the N-glycan structures attached thereto are converted to a MansGlcNAc2 glycoform that can serve as a substrate for additional glycosylation enzymes so that the recombinant glycoprotein produced therein can comprise complex or hybrid N-glycans.