Patent ID: 7998752
Filing Date: 2011-08-16
Classification: G01N,Y10S

Abstract:
1. A blood component analytical method which employs a biosensor that is made of a single layer or plural layers of a porous material which develops shrunk cells, said biosensor having a reagent holding part which holds a reagent for analyzing an analyte in a blood specimen, a carrier, and a reaction layer chromatographically downstream of said carrier wherein said biosensor analyzes target components in the blood specimen by utilizing chromatography, said analytical method comprising the steps of: adding said blood specimen to a specimen addition part of said biosensor; shrinking cell components of said blood specimen using a cell shrinkage reagent of said biosensor that is disposed on at least a part of an area of said biosensor, said area ranging from said specimen addition part to which the blood specimen is added to the reagent holding part thereof, wherein said cell shrinkage agent is dissolved from the area carrying said cell shrinkage reagent by the blood specimen added to the specimen addition part and infiltrating into the area carrying the cell shrinkage reagent and in which the resulting shrunk cell components are smaller; chromatographically permeating the cell components in a state where shrunk cell components included in the blood specimen are mixed with the blood specimen; marking the analyte in the blood specimen which is chromatographically permeated with the reagent which has been held in the reagent holding part, wherein the shrunk cell components are permeated together with the blood specimen toward said reaction layer that is provided chromatographically downstream, wherein the shrunk cell components and the blood specimen are permeated into the reaction layer in a state where each of the shrunk cell components and the blood specimen are mixed; and performing the analysis of the analyte in the blood specimen in said reaction layer in which a reaction of the analyte in the blood specimen and the reagent which is dissolved from the reagent holding part is first carried out, wherein the cell shrinkage agent is sodium chloride, potassium chloride, or sodium phosphate.