Patent ID: 6716610
Filing Date: 2004-04-06
Classification: C12N,C12P

Abstract:
A process for esterifying reaction substrates which use an immobilized lipolytic enzyme which comprises:i) immobilizing a lipolytic enzyme on a porous, anion-exchanging resin carrier by adsorption to provide an immobilized lipolytic enzyme having a water content of 20 to 60% by weight, without drying the immobilized lipolytic enzyme; ii) treating the immobilized lipolytic enzyme formed in step i) by contacting the immobilized lipolytic enzyme containing 20 to 60% by weight of water with fats or oils or a derivative of fats or oils; iii) separating the treated immobilized lipolytic enzyme from the fats or oils in step ii); and iv) esterifying said reaction substrate by contacting the separated immobilized lipolytic enzyme from step iii) with said reaction substrate.