Patent ID: 8312841
Filing Date: 2012-11-20
Classification: A01K

Abstract:
1. A method for culturing and producing mites for preparation of allergenic extracts used in allergy formulations, comprising: a) adding a suitable solid culture medium to a closed bag; b) seeding the suitable solid culture medium with mites; c) piling multiple closed bags on top of each other in a suitable rack system where top surface of a lower closed bag is not touching bottom surface of an upper closed bag; d) culturing the mites in the suitable solid culture medium, under suitable conditions and for a suitable period of time in a suitable production room, wherein the mites are at least one mite selected from the group consisting of the following species: Dermatophagoides pteronyssinus, Dermatophagoides farinae, Dermatophagoides microceras, Dermatophagoides siboney, Tyrophagus putrescentiae, Lepidoglyphus destructor, Glycyphagus domesticus, Acarus siro, Euroglyphus maynei, Blomia kulagini, Blomia tropicalis and Pyroglyphus africanus and e) obtaining allergens from the cultured mites; wherein: i: the closed bag has an oxygen permeability that is sufficient to allow growth of the mites; ii: the closed bag comprises an area which is transparent to allow inspection of the mites growth; iii: the culture medium comprising the growing/cultured mites is present as a layer of less than 110 mm in height; and iv: the closed bag comprises at least 25 g of the culture medium, and further wherein the closed bag is an oxygen permeable bag having an air permeability of at least 150 ml/min as measured according to the standard ISO 5636-3, Bendtsen method; or further wherein the closed bag has an oxygen permeability of at least 10 cm further wherein the closed bag has an oxygen permeability that is sufficient to provide at least the same yield of mites per gram of the culture medium in the closed bag as the yield of mites in a 250 ml Erlenmeyer flask that comprises 30 g of the culture medium and is closed with a cotton plug, wherein the closed bag and the Erlenmeyer flask are cultured under identical conditions.