Patent ID: 8039208
Filing Date: 2011-10-18
Classification: C12Q,G01N

Abstract:
1. A method of identifying and/or quantifying one or more differences in glycosylation between a biological sample affected by a physiological condition and a control sample, comprising (A) obtaining the biological sample, wherein the biological sample contains a plurality of different types of glycoproteins; (B) immobilizing total glycoproteins from the biological sample on a protein binding membrane or in a gel; then (C) releasing glycans of the total glycoproteins immobilized on the protein binding membrane or in the gel without exposing the glycoproteins to hydrazinolysis and without the glycoproteins having been separated into bands prior to said releasing; then (D) washing the protein binding membrane or the gel to separate the released glycans from parent proteins immobilized on the protein binding membrane or in the gel; (E) measuring a glycosylation profile of the glycans; and (F) comparing the glycosylation profile with a glycosylation profile of the control sample to determine the one or more differences in glycosylation between the biological sample affected by the physiological condition and the control sample.