Patent ID: 6835538
Filing Date: 2004-12-28
Classification: C07K,C12N

Abstract:
A method of identifying mutants in a triple gene block 3 (TGB-3) viral sequence which inhibit infection of a virus into a cell, comprising:mutating said TGB-3 sequence; selecting TGB-3 mutants which no longer promote cell-to-cell movement of a (TGB-3 minus) mutant virus when expressed in trans from a replicon; further selecting from the identified mutants those which also inhibit infection with a co-inoculated wild type virus when the mutant TGB-3 is expressed from a replicon; recovering said mutant TGB-3 viral sequence wherein a mutant TGB-3 sequence recovered from the foregoing selection steps is a mutant TGB-3 viral sequence that inhibits infection of a virus into a cell.