Patent ID: 6218154
Filing Date: 2001-04-17
Classification: C12Q

Abstract:
A method for the detection or quantitation of MIP-1.beta. RNA in a sample comprising:a) obtaining a sample which may contain MIP-1.beta. RNA;b) performing isothermal transcription based amplification on the sample with two oligonucleotide primers, a first primer which comprises at least 10 consecutive nucleotides of a sequence selected from the group consisting of SEQ ID NO:26, SEQ ID NO:32, SEQ ID NO:34, and SEQ ID NO:41, and a second primer which comprises at least 10 consecutive nucleotides of a sequence selected from the group consisting of SEQ ID NO:28, SEQ ID NO:30; SEQ ID NO:31, SEQ ID NO:43, and SEQ ID NO:45; andc) detecting or quantitating the resulting product of step b) whereby detection or quantitation of the amplification product indicates the presence or quantity of MIP-1.beta. RNA in the sample.