Patent ID: 6214592
Filing Date: 2001-04-10
Classification: C12N,C12R

Abstract:
An isolated enzyme with amidase activity towards substrates of the amide type having at least one of the following formulae: ##STR14##in which:A and B are monomer units,R.sup.1 and R.sup.3 are identical or different divalent radicals representing a substituted or unsubstituted, linear or branched alkylene, an arylene or an arylalkylene, and the number of carbons in the alkylene being greater than or equal to 4,R.sup.2 corresponds to identical or different radicals selected from hydrogen and alkyl radicals having from 1 to 6 carbons,X is:either X.sup.1 =OH, OM or OR.sup.4, where M is selected from metals, and R.sup.4 is a linear or branched alkyl containing from 1 to 6 carbon atoms,or X.sup.2 = ##STR15##where R.sup.2 and R.sup.3 are as defined above and R.sup.5 and R.sup.6, which are identical or different, have the same definition as that given above for R.sup.2,Y is:either Y.sup.1 =hydrogen,or Y.sup.1 = ##STR16##where R.sup.1 is as defined above and Z is hydrogen, a radical selected from metals or a radical which is a linear or branched alkyl containing from 1 to 6 carbon atoms,with the following conditions:if X=X.sup.1, then Y=Y.sup.1,if X=X.sup.2, then Y=Y.sup.2 or Y.sup.1,and p is between 1.5 and 10;or ##STR17##in which:R.sup.2 and R.sup.3 are as defined above,U and V respectively have the same definitions as those given above for X.sup.1 and Y.sup.1 in formula (I),and q=1 to 8,said enzyme being characterized by a ratioRa=(enzymatic activity towards amide substrates of formula (I)(E.A.(I)))/(enzymatic activity towards amide substrates of formula (II)(E.A.(II))) which is greater than 2.