Patent ID: 6184356
Filing Date: 2001-02-06
Classification: A61K,C07K,C12N,Y10S

Abstract:
A non-naturally occurring pseudotetrameric hemoglobin-like protein comprising a pseudodimeric polypeptide having two substantially homologous globin-like domains, one of which is mutated to provide an asymmetric crosslinkable cysteine residue, wherein the corresponding residue in the other globin-like domain of said pseudodimeric polypeptide is an amino acid other than cysteine, and wherein said pseudotetramer further comprises at least one other globin-like domain-bearing polypeptide, and wherein the asymmetric crosslinkable cysteine residue is a mutation in the alpha globin-like domain corresponding to a human alpha globin position selected from the group consisting of lys7, thr8, lys11, ala12, ,gly15, lys16, gly18, ala19, his20, glu23, and thr24.