Patent ID: 6468751
Filing Date: 2002-10-22
Classification: C12Q

Abstract:
A method of amplifying a single stranded first nucleic acid in a sample potentially containing said first nucleic acid, said method comprising:(a) forming an immersion product, said immersion product comprising said sample and at least one primer set of nucleic acids, said set of nucleic acids comprising at least one second nucleic acid and at least one third nucleic acid, said second nucleic acid and said third nucleic acid being covalently affixed to a support and separated on said support by a distance less than the length of said first nucleic acid, said second nucleic acid being capable of forming a hybridization product with said first nucleic acid, said third nucleic acid being capable of forming a hybridization product with a nucleic acid that is complementary to said first nucleic acid; (b) forming a hybridization product comprising said first nucleic acid and said second nucleic acid, in the event said first nucleic acid is present, by imposing hybridization conditions on the immersion product; (c) forming a first amplification product comprising said second nucleic acid extended by nucleotides complementary to said first nucleic acid, said first amplification product being capable of forming a hybridization product with said third nucleic acid; (d) denaturing the first amplification product from said first nucleic acid; (e) forming a second hybridization product comprising said first amplification product and said third nucleic acid, by imposing hybridization conditions on the immersion product; and (f) forming a second amplification product comprising said third nucleic acid extended by nucleotides complementary to said first amplification product, wherein said first amplification product provides the template for said second amplification product, and said third nucleic acid and said template are immobilized on a common support.