Patent ID: 9102977
Filing Date: 2015-08-11
Classification: C12Q,G01N

Abstract:
1. A method for quantification of nucleic acid of biological cells, said method comprising the steps of: i. providing a sample comprising biological cells, or providing a sample comprising cell parts with nucleic acid, said cell parts being obtained from biological cells, wherein the method is performed without fixation of the biological cells; ii. adding an acidic agent to the sample, the acidic agent comprising a detergent in a buffer having a pH between 2.1-2.9 thereby causing lysis of the biological cells, release of nuclei and inhibition of aggregation of the nuclei; iii. adding a fluorescent labelling agent to the sample, wherein the fluorescent labelling agent interacts with nucleic acid, thereby obtaining a sample with fluorescent labelled nucleic acid; iv. adding a neutralization agent thereby neutralizing the sample after or simultaneously with adding the labelling agent; v. determining the content of fluorescent labelled nucleic acid within the released nuclei; and vi. assessing a result obtained by determining the content of fluorescent labelled nucleic acid to obtain information of the cells in respect of cell cycle analysis, wherein the pH between 2.1-2.9 provides a G