Patent ID: 8236517
Filing Date: 2012-08-07
Classification: C12Q

Abstract:
1. A method for assaying urea in a sample using a device, comprising: moving the sample through a conduit to a sensor in the device comprising immobilized urease and immobilized carbonic anhydrase, wherein the urease and the carbonic anhydrase are immobilized on at least a portion of a single electrode, wherein the sensor is selected from the group consisting of an ammonium ion sensor, a pH sensor, a carbon dioxide sensor, a bicarbonate sensor, and a conductivity sensor, contacting the sample suspected of containing urea with the sensor, and detecting a chemical moiety with the sensor, wherein the device is configured to inhibit carbon dioxide exchange from the sample to an air space in a region of the sensor comprising immobilized urease and immobilized carbonic anhydrase.