Patent ID: 6129788
Filing Date: 2000-10-10
Classification: C12N,C12P

Abstract:
A method of producing one or more monosaccharides and oligosaccharides, comprising the steps of:(a) subjecting a liquefied starch solution to saccharification which comprises more than one or more enzymatic saccharification stages;(b) subjecting the saccharification liquor from one of the saccharification stages to a microfiltration step;(c) subjecting the permeate of the microfiltration step to an ultrafiltration step;(d) re-circulating the retentate from the ultrafiltration step to a saccharification stage of step (a), wherein the saccharide content of said saccharification stage is not significantly lower than the saccharide content in the re-circulated retentate followed by the repetition of steps (b), (c) and (d); and(e) recovering the permeate of the ultrafiltration which comprises the one or more monosaccharides and oligosaccharides.