Patent ID: 6221821
Filing Date: 2001-04-24
Classification: C09D,C11D,C12N

Abstract:
A variant having haloperoxidase activity, an altered pH optimum compared with a haloperoxidase, an amino acid sequence that differs from the amino acid sequence of the haloperoxidase, wherein the amino acid sequence of the haloperoxidase is at least 80% homologous with the haloperoxidase of SEQ ID NO:1 and the difference between the amino acid sequence of the variant and the amino acid sequence of the haloperoxidase comprises:(a) a different amino acid at position 292 wherein the different amino acid is L or E;(b) a different amino acid at position 350 wherein the different amino acid is F or Y;(c) a different amino acid at position 353 wherein the different amino acid is Q or M;(d) a different amino acid at position 360 selected from the group consisting of A, L, I, Q, M, E and D;(e) a different amino acid at position 394 selected from the group consisting of A, L, I, Q, M, E and D;(f) a different amino acid at position 395 wherein the different amino acid is A or S;(g) a different amino acid at position 397 selected from the group consisting of N, Y, E, and Q;(h) a different amino acid at position 399 wherein the different amino acid is G;(i) a different amino acid at position 402 selected from the group consisting of A, T, and V;(j) a different amino acid at position 490 selected from the group consisting of A, L, I, Q, M, E, and D;(k) a different amino acid at position 495 selected from the group consisting of A, T, and S; and/or(l) a different amino acid at position 501 wherein the different amino acid is S, wherein each position corresponds to the position of the amino acid sequence of the haloperoxidase of SEQ ID NO:1.