Patent ID: 7074404
Filing Date: 2006-07-11
Classification: A61K,A61P,C07K

Abstract:
1. A composition comprising a mixture of anti-HER2 antibody and one or more acidic variants thereon wherein the amount of acidic variant(s) is less than about 13%, and wherein the composition has been subjected to cation exchange chromatography comprising the following steps performed sequentially: (a) binding the anti-HER2 antibody to a cation exchange material using a loading buffer at a first conductivity; (b) washing the cation exchange material with an intermediate buffer at a second conductivity which is greater than that of the loading buffer so as to elute acidic variant(s) from the cation exchange material; (c) washing the cation exchange material with a wash buffer which is at a third conductivity which is less than that of the intermediate buffer; and (d) washing the cation exchange material with an elution buffer at a fourth conductivity which is greater than that of the intermediate buffer so as to elute the anti-HER2 antibody from the cation exchange material.