Patent ID: 6309836
Filing Date: 2001-10-30
Classification: C07H,C12Q

Abstract:
A method for sequencing a nucleic acid comprising the steps of:a) contacting a target nucleic acid with a primer under conditions wherein said primer anneals to said target nucleic acid in a sequence specific manner and wherein at least a portion of said primer is complementary to a portion of said target nucleic acid;b) incorporating a hydrocarbyldithiomethyl-modified nucleotide into said primer; andc) detecting incorporation of said hydrocarbyldithiomethyl-modified nucleotide, wherein said hydrocarbyldithiomethyl-modified nucleotide is complementary to said target nucleic acid at said hydrocarbyldithiomethyl-modified nucleotide's site of incorporation thereby identifying the sequence of one nucleobase of said target nucleic acid.