Patent ID: 7935510
Filing Date: 2011-05-03
Classification: A61K,C07D,C07H,C07K,C12N,Y10S

Abstract:
1. A recombinant gene expression modulation system comprising a recombinant gene expression cassette that is capable of being expressed in a host cell, the gene expression cassette comprising a polynucleotide that encodes a first polypeptide comprising: (a) a transactivation domain; (b) a DNA-binding domain that recognizes a response element associated with a gene whose expression is to be modulated; and (c) a nuclear receptor ligand binding domain that is capable of binding a diacylhydrazine and comprises either an amino acid residue substitution mutation of SEQ ID NO: 1 at at least one of the mutations F48Y, F48W, F48L, F48N, F48R, F48K, I51M, I51N, 151L, T52M, T52E, T52P, T52R, T52W, T52G, T52Q, M92L, M92E, R95H, R95M, R95W, V96L, V96W, V96S, V96E, V96T, V1071, F109W, F109P, F109L, F109M, F109N, A110E, A110N, A110P, A110W, Y120W, Y120M, M125P, M125R, M125E, M125L, M125C, M125W, M125G, M125I, M125N, M125S, M125V, Y127E, M219K, M219W, M219Y, M219A, L223K, L223R, L223Y, L234M, L2341, L234R, L234W, W238P, W238E, W238Y, W238M and W238L.