Patent ID: 7125682
Filing Date: 2006-10-24
Classification: C12Q

Abstract:
1. A method for determining phosphorylating activity of an enzyme acting on a substrate molecule, said substrate comprising at least one moiety that is phosphorylated by said enzyme to yield a phosphorylated product and wherein said substrate is labelled with a fluorescent dye, said method comprising the steps of: i) measuring fluorescence intensity of the fluorescently labelled substrate; ii) combining said enzyme with said substrate molecule in the presence of a phosphate donor; and iii) measuring an increase in fluorescence intensity of the fluorescent label following step ii); wherein said fluorescent dye is selected from the group consisting of acridone and quinacridone classes of dyes; and wherein said increase in fluorescence intensity of the fluorescent label is used to determine the phosphorylating activity of said enzyme.