Patent ID: 8716557
Filing Date: 2014-05-06
Classification: A61P,C07K,C12N

Abstract:
1. A nucleotide construct comprising a first nucleotide sequence that is capable of inhibiting expression of an α1,3-fucosyltransferase (FucT) in a duckweed plant, and a second nucleotide sequence that is capable of inhibiting expression of a β1,2-xylosyltransferase (XylT) in a duckweed plant, wherein said first nucleotide sequence is operably linked to a first promoter that is functional in a plant cell, and wherein said second nucleotide sequence is operably linked to a second promoter that is functional in a plant cell, wherein said first nucleotide sequence comprises in the 5′-to-3′ orientation and operably linked: (a) a FucT forward fragment, said FucT forward fragment comprising about 500 to about 800 contiguous nucleotides having at least 90% sequence identity to a nucleotide sequence of about 500 to about 800 contiguous nucleotides of SEQ ID NO:1 or SEQ ID NO:2; (b) a spacer sequence comprising a nucleotide fragment of the sequence set forth in SEQ ID NO:1; and (c) a FucT reverse fragment, said FucT reverse fragment having sufficient length and sufficient complementarity to said FucT forward fragment such that said first nucleotide sequence is transcribed as an RNA molecule capable of forming a hairpin RNA structure.