Patent ID: 8846318
Filing Date: 2014-09-30
Classification: C12Q

Abstract:
1. A method for identifying an olfactory receptor included in one olfactory cell among mouse olfactory receptor group A, the method comprising the steps of: (a) obtaining the one olfactory cell; (b) extracting mRNA contained in the one olfactory cell obtained in step (a); (c) obtaining cDNA with reverse transcriptase from the mRNA extracted in step (b); (d) amplifying the cDNA obtained in step (c) by a PCR method using primers of SEQ ID NO: 1 and SEQ ID NO: 2; wherein SEQ ID NO:1 consists of A (adenine) -T (thymine) -G (guanine) -K 15 (guanine or thymine) -C (cytosine) -I (inosine) -C (cytosine) -T (thymine) -I (inosine) -G (guanine) -A (adenine) -Y (thymine or cytosine) -M (adenine or cytosine) -G (guanine) -I (inosine) -T (thymine) -A (adenine) -Y (thymine or cytosine) -G (guanine) -T (thymine) -I (inosine) -G (guanine) -C (cytosine); and SEQ ID NO: 2 consists of T (thymine) -G (guanine) -Y (thymine or cytosine) -T (thymine) -T (thymine) -I (inosine) -G (guanine) -T (thymine) -Y (thymine or cytosine) -Y (thymine or cytosine) -T (thymine) -I (inosine) -A (adenine) -C (cytosine) -I (inosine) -C (cytosine) -Y (thymine or cytosine) -R (guanine or adenine) -T (thymine) -A (adenine) -I (inosine) -A (adenine) -T (thymine) -I (inosine) -A (adenine) -T (thymine) -I (inosine) -G (guanine) -G (guanine) -R (guanine or adenine) -T (thymine) -T (thymine); and (e) determining whether or not the sequence of the cDNA amplified in step (d) is identical to the gene sequences coding for olfactory receptors included in the mouse olfactory receptor group A; wherein the mouse olfactory receptor group A gene sequences include the following mouse olfactory receptors: Olfr17, Olfr64, Olfr67, Olfr68, Olfr155, Olfr168, Olfr510, Olfr544, Olfr545, Olfr548-ps1, Olfr551, Olfr560, Olfr566, Olfr568, Olfr569, Olfr578, Olfr582, Olfr583, Olfr584, Olfr585, Olfr606, Olfr611, Olfr617, Olfr619, Olfr625-ps1, Olfr630, Olfr640, Olfr646, Olfr649, Olfr652, Olfr665, Olfr666, Olfr667, Olfr668, Olfr995, Olfr1101, and Olfr1402.