Patent ID: 8628667
Filing Date: 2014-01-14
Classification: B01D,B01J,G01N,Y10T

Abstract:
1. A method of constructing a chromatography column, comprising: providing a column tube, wherein said column tube has a first end, a second end, an interior wall and an exterior wall, wherein said tube has a tube chamber and wherein said tube has a recess machined in said first end; securing a medium collar having a top end, a bottom end, a substantially smooth inner surface, and an articulated external surface in said recess with said external surface abutting said recess, said inner surface substantially aligned with said interior wall and said top end aligned with said first end, wherein said tube with said medium collar installed has an interior chamber; affixing an outlet filter assembly to said second end of said column tube, wherein said outlet filter assembly has a second housing for mounting to said second end and a fluid outlet that is in fluid communication with said housing, wherein a first column sorbent bed volume is defined by said interior chamber and said affixed outlet filter assembly; adding a separation medium into said interior chamber of said column tube; inserting an inlet filter assembly onto said first end, said inlet filter assembly having a first housing for mounting to said first end, a fluid inlet that is in fluid communication with said first housing, and end seal fitted to said first housing for mating with said tube first end, and a protruding portion that extends from said first housing into said interior chamber, said protruding portion cooperating with said medium collar to retain said separation medium in said chamber; and affixing said inserted inlet filter assembly to said first end, wherein a second column sorbent bed volume is defined by said tube affixed to said outlet filter assembly and said inlet filter assembly, and wherein said second bed volume is less than said first bed volume.