Patent ID: 6198023
Filing Date: 2001-03-06
Classification: A01H,C07K,C12N,C12Y,Y10S

Abstract:
An isolated promoter, derived from the group consisting of the promoters of the L4, L10, L16, and L19 genes, wherein said promoter functions to regulate the expression of DNA preferentially in a plant cell critical to pollen formation or function, and wherein said promoter is the product of a method comprising(A) isolating a first set of mRNAs from cells or tissues critical to pollen formation or function and then using said first set of mRNAs to prepare a first set of radiolabeled probes;(B) isolating a second set of mRNAs from cells or tissues that are not critical to pollen formation or function and then using said second set of mRNAs to prepare a second set of radiolabeled probes:(C) screening a plant genomic library with said first and second sets of radiolabeled probes, to identify a clone that hybridizes only to said first set of radiolabeled probes, and then(D) identifying a promoter in said clone.