Patent ID: 7556945
Filing Date: 2009-07-07
Classification: C12P

Abstract:
1. A method for converting sucrose to β-D-glucose, comprising the steps of: a) providing a sucrose containing solution having a pH of between about 5.8 and about 4.6; b) heating the sucrose containing solution to a temperature of about 43° C. to about 47° C.; c) contacting the sucrose containing solution with invertase, wherein the conditions of pH and temperature are maintained, to provide a fructose and α-D-glucose containing solution; d) contacting the fructose and α-D-glucose containing solution with glucose isomerase, wherein the conditions of pH and temperature are maintained, to provide a solution containing α-D-glucose and β-D-glucose; and e) contacting the α-D-glucose and β-D-glucose with mutarotase, wherein the conditions of pH and temperature are maintained, to provide a solution containing a high percentage of β-D-glucose.