Patent ID: 8969002
Filing Date: 2015-03-03
Classification: B01L,B03C,C12Q,C40B,G01N,H01L

Abstract:
1. A method for sequencing a nucleic acid template, comprising: a) contacting the nucleic acid template hybridized to a primer and coupled to a particle, which particle is proximate to a nanosensor in a nanosensor array, with a fluid containing nucleotides, wherein said nanosensor comprises a pair of electrodes; b) using the pair of electrodes to detect a change in conductivity within the Debye length of the particle upon incorporation of at least one nucleotide into a growing nucleic acid strand that is complementary to the nucleic acid template; c) washing said nanosensor array to remove excess nucleotides; and d) repeating steps a, b, and c to obtain sequence information for said nucleic acid template.