Patent ID: 6398719
Filing Date: 2002-06-04
Classification: A61B,B01L,G01N

Abstract:
A method for sperm washing and concentration comprising the steps of:i) providing a tube for sperm washing and concentration comprising a glass tube having a top portion and a bottom portion, an open end being provided in the top portion and the bottom portion being closed and having a reduced diameter relative to said top portion, and an elastic bladder removably provided at the open end of the tube, wherein said bottom portion having a reduced diameter has a weakened portion for bending and removing said bottom portion, ii) filling the rube for sperm washing and concentration with a density gradient carrier, iii) diluting semen with an equivalent volume of HANKS solution or physiological saline, iv) providing the semen of step iii) onto a filter for removing fiber, gelatin-like mass and urolithiasis therein and layering the filtrated semen onto the density gradient carrier in the glass tube, v) after layering the whole volume of the semen onto the density gradient carrier, stirring both sides of an interface between the semen and the density gradient carrier to remove the interface, vi) centrifuging the tube of step v), vii) after the centrifugation, providing said elastic bladder in a compressed state at the open end of the glass tube and obtaining a sediment containing the washed and concentrated semen and the density gradient carrier by removing the bottom portion with a small diameter of the tube, and viii) removing the density gradient carrier to leave essentially only the sediment.