Patent ID: 8816153
Filing Date: 2014-08-26
Classification: C12N

Abstract:
1. A recombinant DNA construct comprising: (a) a promoter operably linked to a first, sense-oriented, DNA sequence, wherein the first, sense-oriented DNA sequence encodes a protein; (b) a second DNA sequence that is complementary to at least a fragment of said first DNA sequence, wherein the second DNA sequence is at least 21 nucleotides in length and wherein the second DNA sequence is in the anti-sense orientation with respect to said promoter and the first DNA sequence; (c) at least one DNA sequence providing a polyadenylation sequence; and (d) a pair of recombination sites which are recognized by a site-specific recombinase, wherein at least one of the pair of the recombination sites is located between said first DNA sequence and said second DNA sequence and wherein at least one of the pair of the recombination sites is located between said second DNA sequence and said at least one DNA sequence providing a polyadenylation sequence; wherein expression of said construct in a cell having endogenous DNA encoding said protein produces a polyadenylated transcript, and can be switched between enhanced expression of said protein and suppression of said endogenous DNA by regulating the presence of a site-specific recombinase.