Patent ID: 8945852
Filing Date: 2015-02-03
Classification: G01N

Abstract:
1. A method for performing homogeneous immunoassay formats with electrochemical detection in solution for the detection of an analyte in a sample comprising: (a) combining a water-soluble first conjugate and a second conjugate as reagents with the sample or the sample in a buffer solution, the first conjugate comprising a redox marker and the analyte conjugated via a linker and the second conjugate comprising an anti-redox marker antibody or a fragment that binds the redox marker and a molecule specifically binding the analyte conjugated via a linker, (b) binding the first conjugate and the second conjugate, whereby a detectable signal is generated or changed, and (c) detecting the signal; wherein an affinity constant of the molecule specifically binding the analyte is greater than an affinity constant of the anti-redox marker antibody or fragment that binds the redox marker.