Patent ID: 8445217
Filing Date: 2013-05-21
Classification: G01N

Abstract:
1. A method for free-solution determination of molecular interactions comprising the steps of: (a) providing a substrate having a channel formed therein for reception of a fluid sample to be analyzed; (b) introducing a first sample comprising a first non-immobilized analyte to be analyzed into the channel; (c) introducing a second sample comprising a second non-immobilized analyte to be analyzed into the channel; (d) allowing the first analyte to interact with the analyte to form one or more interaction products; (e) directing a coherent light beam onto the substrate such that the light beam is incident on the channel to generate backscattered light through reflective and refractive interaction of the light beam with a substrate/channel interface and the sample, the backscattered light comprising interference fringe patterns including a plurality of spaced light bands whose positions shift in response to changes in the refractive index of the fluid sample; (f) detecting positional shifts in the light bands; and (g) determining an equilibrium constant of the interaction from the detected shifts.