Patent ID: 8993320
Filing Date: 2015-03-31
Classification: A61P,C12N

Abstract:
1. An enriched population of expanded human neural cells wherein the cells are progenitor cells or stem cells, wherein the cells have been cultured under conditions effective to enrich for the expanded neural cells, said conditions comprising, a culture medium comprising an effective amount of a growth supplement, wherein the growth supplement is 1-thioglycerol and the effective amount of growth supplement in the culture medium is at least 10 μM of 1-thioglycerol to isolate human fetal spinal cord (“HFSC”) cells, wherein the cells maintain their capability to differentiate into neurons, astrocytes, and oligodendrocytes, wherein the cells maintain their ability to differentiate into oligodendrocyte lineage cells efficiently throughout subsequent passages, and wherein the population of cells express at least cell surface antigens CD133, CD140a, A2B5 and PSA-NCAM, and wherein at least about 90% of cells of the enriched population of expanded cells express the cell surface antigen CD133, CD140a, and A2B5, and at least about 60% of the enriched population of expanded cells also express the cell surface antigen PSA-NCAM.