Patent ID: 6635440
Filing Date: 2003-10-21
Classification: C12N,C12Q,G01N

Abstract:
A method for the identification of a compound that alters the affinity of interaction between an RNA-binding site and an RNA-binding compound, said method comprising the steps of:a) expressing in a host cell i) a nucleic acid comprising a coding region for the RNA-binding compound, ii) a nucleic acid comprising a transcribed sequence comprising a coding region for a fluorescent marker protein, wherein the nucleic acid comprising the coding region for the fluorescent marker protein includes a binding site for the RNA-binding compound within a transcript transcribed from the transcribed sequence, wherein binding of the RNA-binding compound to the RNA-binding site represses translation of the transcribed sequence, b) contacting said host cell with a compound to be screened for altering the affinity of interaction between the RNA-binding site and the RNA-binding compound, and c) measuring the level of fluorescence in said host cells, wherein a compound that alters said level of fluorescence from the level that is seen in the absence of compound is indicative of a compound that alters the affinity of interaction between the RNA-binding site and the RNA-binding compound.