Patent ID: 8921279
Filing Date: 2014-12-30
Classification: C07K,C40B,G01N

Abstract:
1. A method of producing a functional binding agent, wherein the functional binding agent binds to a target antigen, wherein the functional binding agent is an antibody Fc fragment consisting of a CH2 domain and a CH3 domain, comprising the steps of: a) providing a library of said antibody Fc fragments, b) contacting said library with said target antigen in the presence of a scaffold ligand, wherein said target antigen and said scaffold ligand are different molecules, wherein said scaffold ligand is selected from the group consisting of CD64, CD16, CD32, FcRn and Protein A, c) selecting a library member binding to said target antigen in the presence of said scaffold ligand to obtain a functional binding agent, and d) producing a preparation of the functional binding agent.