Patent ID: 6799121
Filing Date: 2004-09-28
Classification: G01N,G16B

Abstract:
A method of analyzing argentinated peptides or argentinated proteins using mass spectrometry comprising: (a) combining an oligopeptide with silver to provide a sample comprising argentinated peptide; (b) submitting the sample to a mass spectrometer; (c) performing scans of silver containing peaks in optimum collision energies to maximize doublet or triplex peak patterns, wherein the scans are performed; (i) under increasing collision energies, identifying doublet and triplet peak patterns and determining the collision energies that maximized the doublet and/or triplet peak patterns, summing the spectra information from said optimum collision energies to produce a composite spectra that maximizes the doublet and/or triplet peak patterns: or (ii) over a range of collision enemies to maximize the doublet and/or triplet peak patterns; (d) identifying any doublet or triplet peak pattern; (e) confirming with Y ions; and (f) determining partial sequence by the mass separation between two successive doublet or triplet patterns.