Patent ID: 6821770
Filing Date: 2004-11-23
Classification: C12Q

Abstract:
A device for detecting nucleic acids, comprisinga solid support; and a plurality of addresses disposed on the solid support, each address comprising at least one detectably labeled probe that hybridizes ribosomal nucleic acids from at least one microbial species under high stringency hybridization conditions, said plurality of addresses comprising, a pan-bacterial address, a higher-order address, wherein said higher-order address is a pan-fungal address that specifically hybridizes ribosomal nucleic acids from a plurality of fungal species, an intermediate-order address, and a lower-order address, wherein the lower-order address hybridizes ribosomal nucleic acids from a subset of organisms having ribosomal nucleic acids that hybridize at the intermediate-order address, wherein the intermediate-order address hybridizes ribosomal nucleic acids from a subset of organisms having ribosomal nucleic acids that hybridize at the higher-order address, and wherein at least one of said intermediate-order address and said lower-order address further comprise a detectably labeled probe that hybridizes ribosomal nucleic acids from a microbial species having ribosomal nucleic acids that hybridize at the pan-bacterial address.