Patent ID: 8741277
Filing Date: 2014-06-03
Classification: A61K,A61P,C12N

Abstract:
1. A method of augmenting or bulking non-muscle soft tissue in a mammalian subject consisting essentially of: administering a physiologically acceptable composition comprising a carrier, excipient, or diluent, and isolated skeletal muscle-derived progenitor cells (MDCs) to the soft tissue in an amount sufficient to augment or bulk the tissue, wherein the MDCs are isolated using a method comprising: (a) plating a suspension of skeletal muscle cells from skeletal muscle tissue in a first container containing cell culture media, wherein fibroblast cells of the skeletal muscle cell suspension adhere to the wall of the first container; (b) re-plating non-adherent cells from step (a) after about one hour in a second container containing media, wherein 30-40% of the non-adherent cells transferred from the first container adhere to the wall of the second container; (c) re-plating the non-adherent cells from step (b) serially in four containers containing media, wherein the step of re-plating is after 30-40% of cells have adhered to the wall of each container; and (d) isolating the non-adherent cells transferred from the fifth container from the wall of the sixth container following the re-plating in step (c), wherein the isolated cells are MDCs expressing CD34