Patent ID: 7579023
Filing Date: 2009-08-25
Classification: A61K,A61P

Abstract:
1. A method for isolating a bioactive component of a water soluble extract of an Uncaria species, wherein said extract exhibits UV maxima at approximately 199 nm, is stable to temperatures less than 100° C. for 24 hours and sterilization by autoclaving for up to at least 20 minutes at approximately 121° C., maintains biological activity for at least 6 months when frozen in liquid form at approximately −20° C. and consists essentially of molecules having a molecular weight of up to approximately 12,000, comprising: (a) removing alcohol insoluble components from said water soluble extract by precipitation with a first alcohol solution; (b) spotting said water soluble extract resulting from step (a) on pre-run thin layer chromatography (TLC) plates and chromatographing the spotted plates in a system of approximately 1% ammonia in a second alcohol solution, thereby obtaining a fluorescing band with an R (c) separating said fluorescing band; (d) eluting said separated fluorescing band with aqueous ammonia and drying said eluted separated flourescing band to form a solid; (e) extracting said solid with a third alcohol solution to remove non-alcohol solubilized silica gel, leaving said third alcohol solution; (f) concentrating said third alcohol solution; and (g) separating said bioactive component from said concentrated third alcohol solution.