Patent ID: 8501431
Filing Date: 2013-08-06
Classification: A61P,C12Q,G01N

Abstract:
1. A method for screening for compounds having anti-cancer activity comprising (a) providing cells having one or more mutations in the cell cycle checkpoint protein complex that includes Rad9, wherein the cells have a mutation in the Rad9 gene, and wherein the cells are defective in the Rad9 protein-mediated cell cycle arrest, (b) adding a compound to be screened to the cells, wherein the compound has the structure of Formula Ia: wherein R alkyl, substituted alkyl, alkenyl, substituted alkenyl, alkynyl, substituted alkynyl, phenyl, substituted phenyl, aryl, substituted aryl, heteroaryl, substituted heteroaryl, halo, hydroxyl, alkoxy, phenoxy, substituted phenoxy, aroxy, substituted aroxy, alkylthio, phenylthio, substituted phenylthio, arylthio, substituted arylthio, cyano, isocyano, substituted isocyano, carbonyl, carboxyl, substituted carboxyl, amino, substituted amino, amido, substituted amido, sulfonyl, sulfonic acid, phosphoryl, phosphonyl, polyaryl, substituted polyaryl, C Z is a heteroatom selected from oxygen, sulfur, or nitrogen; and X is a heteroatom selected from oxygen, sulfur, or nitrogen (c) determining if the compound kills or induces apoptosis in some or all of the cells; (d) comparing the resultant determination of step (c) to the resultant determination induced by the compound in normal cells not having the one or more mutations in (a) to determine if the compound has anti-cancer activity.