Patent ID: 7018814
Filing Date: 2006-03-28
Classification: A61P,C07K

Abstract:
1. A method of purifying vancomycin HCl from a fermentation broth of a microorganism containing vancomycin, comprising: passing the fermentation broth of a microorganism containing vancomycin through a strong acid cation exchange resin at pH 1–3 and eluting vancomycin with a 0.05 to 0.2 N ammonium hydroxide solution in water having a pH of 9–11; adjusting a pH of the eluted solution to 3–5, passing the solution through a weak base anion exchange resin and subsequently alumina, and then washing the weak base anion exchange resin and alumina with water to elute vancomycin, and decolorizing vancomycin; passing the eluted solution through a hydrophobic absorbent resin and eluting vancomycin with an aqueous solution of C1–4 alcohol; and adding hydrochloric acid to the eluted solution to adjust the pH of the eluted solution to 2–5 and adding an water-soluble organic solvent selected from the group consisting of a C1–4 alcohol, acetonitrile, acetone, and methyl isobutyl ketone to the eluted solution to crystallize vancomycin HCl.