Patent ID: 6071730
Filing Date: 2000-06-06
Classification: A61K,C07K,C12N

Abstract:
A method for making a plant resistant to a plant pathogen, said method comprising transforming a plant cell with a polynucleotide molecule, said molecule encoding a trans-splicing Group I ribozyme, the sequence of said ribozyme being a fusion RNA, the sequence of said fusion RNA comprising:(1) a first RNA sequence, which hybridizes to a target RNA that encodes a transcription activator protein, said transcription activator protein being specifically produced in pathogen-specific tissue; and(2) a second RNA sequence being a sequence to be trans-spliced into said target RNA;wherein said polynucleotide molecule is operably linked to a transcription regulatory element which is specifically recognized by said transcription activator protein such that association of said transcription activator protein with said transcription regulatory element results in activation of transcription of said polynucleotide molecule and production of said trans-splicing ribozyme only in said pathogen-specific tissue, wherein said trans-splicing ribozyme provides said plant with resistance to said pathogen, wherein infection of a cell from said plant with said pathogen results in the ablation of said cell.