Patent ID: 6911311
Filing Date: 2005-06-28
Classification: C12N,G01N

Abstract:
1. A method for detecting an interaction between a first test agent and a second test agent, comprising: providing a first fusion construct and a second fusion construct, said first fusion construct having an N-intein and said first test agent, said second fusion construct having a C-intein and said second test agent, wherein at least one of the two fusion constructs has an inactive reporter capable of being converted to an active reporter upon trans-splicing through said N-intein and said C-intein, and wherein said N-intein and said C-intein do not interact with each other; allowing said first test agent in said first fusion construct to interact with said second test agent in said second fusion construct in vitro; and detecting said active reporter thereby detecting a an interaction between the first and second test agent.