Patent ID: 8932648
Filing Date: 2015-01-13
Classification: A61K,G01N

Abstract:
1. A method to determine whether a candidate compound is capable of binding phosphate, comprising steps of: a) contacting the candidate compound with phosphate under conditions permitting binding of the candidate compound with phosphate; b) measuring reduction of phosphate concentration in the presence of the candidate compound in a variety of buffer or solution; c) contacting the candidate compound with Caco-2 cells in a phosphate solution; d) measuring reduction of phosphate absorption in the cells in the presence and absence of the candidate compound; and e) correlating reduction of phosphate concentration in solution with reduction of phosphate absorption in the Caco-2 cells, wherein a candidate compound that reduces both the phosphate concentration in solution and phosphate absorption in the Caco-2 cells is a phosphate binder.