Patent ID: 8021847
Filing Date: 2011-09-20
Classification: C12Q

Abstract:
1. A method of isolating and confirming the presence of a mammalian cellular RNA in a plurality of extracellular microvesicles, the method comprising the steps of: providing a sample that includes a plurality of mammalian cells and a plurality of extracellular microvesicles; separating the extracellular microvesicles from the plurality of mammalian cells, and differentially isolating a sub-population of microvesicles from the extracellular microvesicles using a cell-specific or an organ-specific marker; detecting the mammalian RNA in the extracellular microvesicles or in the sub-population of microvesicles using a step of hybridization or amplification; and using the step of detecting the mammalian RNA to confirm the presence of the RNA, wherein the mammalian RNA is a known marker of a disease.