Patent ID: 7319020
Filing Date: 2008-01-15
Classification: C12N

Abstract:
1. A method for obtaining a protein from a transgenic host potato plant, wherein the gene coding for said protein is not expressed until said transgenic host potato plant has been harvested, said method comprising the steps of: a) obtaining a transgenic host potato plant comprising a gene coding for a protein wherein said gene is functionally linked to an anaerobically inducible promoter and said gene is only expressed upon anaerobic induction; b) cultivating said transgenic host potato plant; c) harvesting said transgenic host potato plant thereby obtaining harvested transgenic host potato plant tissue; d) transferring said harvested transgenic host potato plant tissue into a reaction container; e) displacing a gas phase in said reaction container by introducing a gaseous inductor selected from the group consisting of nitrogen and carbon dioxide into said reaction container, thereby producing anaerobic conditions; f) expressing said protein in said harvested transgenic host potato plant tissue by anaerobic induction of said anaerobically inducible promoter; and g) recovering said protein expressed in step f) from said harvested transgenic host potato plant tissue.