Patent ID: 7517664
Filing Date: 2009-04-14
Classification: C12N

Abstract:
1. A method for the determination of an analyte in a sample, comprising: (a) providing a reagent comprising a glucose dehydrogenase variant of SEQ ID NO: 6, wherein said variant differs from SEQ ID NO: 6 by an amino acid substitution at position 145 or 158, said variant having less than 3% residual enzymatic activity relative to the wild type enzyme of (b) contacting a sample suspected to contain an analyte with the reagent in the presence of a coenzyme, under conditions wherein the amount of the coenzyme bound to the glucose dehydrogenase variant to form a coenzyme glucose dehydrogenase variant complex is indicative of the amount of analyte to be determined; and (c) detecting the coenzyme glucose dehydrogenase variant complex.