Patent ID: 6297008
Filing Date: 2001-10-02
Classification: C07D,C12Q

Abstract:
A process for detecting a target single-stranded nucleic acid having a first base sequence, said process comprising the steps of:(a) forming a double-stranded nucleic acid by hybridizing said target single-stranded nucleic acid with a probe nucleic acid having a second base sequence complementary to the first base sequence;(b) associating a chemiluminescent compound with the double-stranded nucleic acid;(c) exposing the chemiluminescent compound associated with said double-stranded nucleic acid to at least two luminescence-inducing compounds which generate energy which excites the associated chemiluminescent compound into luminescence; and(d) detecting the luminescence from the chemiluminescent compound excited in step (c), wherein said step (c) is conducted such that (i) only the associated chemiluminescent compound emits luminescence, and (ii) the detected luminescence is indicative of the target single-stranded nucleic acid and wherein said chemiluminescent compound is a compound represented by the following formula (7): ##STR24##wherein X is O, S, Se, or Te, and Y- is an anion.