Patent ID: 8529750
Filing Date: 2013-09-10
Classification: B01L,G01N,Y10T

Abstract:
1. A method for detecting biomolecules, the method comprising: providing a FET comprising a substrate, a source electrode, a drain electrode, a channel region between the source and drain electrodes, and probe molecules fixed to the channel region, wherein the source and drain electrodes are space apart from each other on the substrate; supplying a reference buffer solution of low ionic concentration to the channel region to which the probe molecules are fixed, and measuring a first current value flowing through the channel region when a reference buffer solution of low ionic concentration is supplied to the channel region to which the probe molecules are fixed; binding the target molecules to the probe molecules by supplying a reaction solution of high ionic concentration containing the target molecules to the channel region to which the probe molecules are fixed; removing the reaction solution of high ionic concentration after reaction by supplying the reference buffer solution of low ionic concentration to the channel region where the target molecules bind to the probe molecules; measuring a second current value flowing through the channel region when the reference buffer solution of low ionic concentration is supplied to the channel region where the target molecules bind to the probe molecules; and detecting the target molecules using a difference between the first and second current values.