Patent ID: 9193967
Filing Date: 2015-11-24
Classification: A61K,A61P,C12N,C12Y

Abstract:
1. A method for preparing a PEGylated analogue protein of canine urate oxidase, comprising: a. preparing a urate oxidase protein comprising more than 95.0% of tetramer analogue protein of canine urate oxidase prior to the PEGylation, wherein said analogue protein of canine urate oxidase is a chimeric protein which comprises amino acids of a canine urate oxidase and amino acids of a human urate oxidase, wherein the first 240 amino acids at the N-terminal of said chimeric protein are amino acids 1-240 of the canine urate oxidase and the following 64 amino acids are amino acids 241-304 of the human urate oxidase; b. PEGylating said analogue protein of canine urate oxidase, wherein the average molecular weight of said PEG is 1 kD˜40 kD, and each monomer of said analogue protein of canine urate oxidase is conjugated in average with 2˜15 PEG molecules; c. separating and purifying said PEGylated analogue protein.