Patent ID: 6586208
Filing Date: 2003-07-01
Classification: C12N

Abstract:
A method for producing adenovirus, comprising:(a) producing a host cell which expresses a gene product or where expression of said gene product requires an accessory protein, wherein said gene product, said accessory protein or both are required for adenovirus production and are toxic to said cell, by introducing a termination codon into a nucleic acid encoding said gene product or a nucleic acid encoding said accessory protein in said cell to down regulate production of said gene product or said accessory protein; (b) introducing into said host cell, adenovirus packaging plasmids for expressing components of adenovirus particles; (c) expressing a tRNA in said cell, wherein said tRNA comprises an anticodon for said introduced termination codon and an amino acid for incorporation into a polypeptide during translation to suppress said introduced termination codon; and (d) retrieving adenovirus produced by said host cell.