Patent ID: 7192733
Filing Date: 2007-03-20
Classification: A61P,C12N,G01N

Abstract:
1. An assay method for identifying a modulator of Prostaglandin endoperoxidase (PGE) synthase activity comprising: a) providing a PGE synthase-containing membrane fraction from cells recombinantly producing said PGE synthase; b) incubating said membrane fraction in the presence and absence of a test compound in the presence of a cyclic endoperoxide substrate of PGE synthase under conditions in which PGE synthase normally catalyzes conversion of the cyclic endoperoxide substrate into a product with is the 9-keto, 11α hydroxyl form of the substrate; and c) determining PGE synthase activity in the presence and absence of said test compound; wherein said polypeptide which is a PGE synthase comprises the amino acid sequence of SEQ ID NO: 2 or consists of a portion of the amino acid sequence of SEQ ID NO: 2 having PGE synthase activity.