Patent ID: 9133438
Filing Date: 2015-09-15
Classification: A61K,A61P,C12N,G01N,Y02A

Abstract:
1. A method of generating a stem cell, comprising: obtaining brown fat tissue; isolating a stem cell from the brown fat tissue; and culturing the stem cell in a differentiation medium not comprising a xenogenic animal product, wherein the differentiation medium comprises, insulin, dexamethasone, isobutylmethylxanthine, indomethacin, triiodothyronine (T3), rosiglitazone, fibronectin type III domain-containing protein 5 (FNDC5), and 0.5 to 20% human platelet lysate; thereby generating a stem cell, wherein the stem cell is positive for one or more of the following cell surface markers: CD63, CD90, HLA, ABC, CD105, CD73, CD166, CD9, CD44, and wherein the stem cell is negative for one or more of the following cell surface markers: CD34, CD19, CD86, HLA DR, Lin, CD106, CD80, CD117.