Patent ID: 9221887
Filing Date: 2015-12-29
Classification: A61K,A61P,C07K,C12N,G01N

Abstract:
1. A mutein of human tear lipocalin having detectable binding affinity to a given non-natural ligand and of human tear lipocalin, wherein at least one of the cysteine residues occurring at sequence positions 61 and 153 of the linear wild type amino acid sequence of mature human tear lipocalin (SEQ ID NO: 71) is replaced by other amino acids and wherein at least 12, 14, or 16 mutated amino acid residues are present at any of the sequence positions 26-34, 56-58, 80, 83, 104-106, and 108 of the linear wild type amino acid sequence of mature human tear lipocalin set forth in SEQ ID NO: 71, wherein the non-natural ligand is vascular endothelial growth factor (VEGF) or a fragment thereof.