Patent ID: 6180348
Filing Date: 2001-01-30
Classification: C12Q

Abstract:
A method for identifying an aptamer which specifically binds to a target molecule other than a nucleic acid, comprising the steps of:a) providing a collection of oligonucleotides attached to a plurality of solid supports, wherein oligonucleotides of different nucleotide sequences are attached to different solid supports and all oligonucleotides attached to a single solid support are of the same nucleotide sequence;b) providing a plurality of magnetic beads having said target molecule immobilized on their surface;c) admixing said collection of oligonucleotides with said plurality of magnetic beads under conditions wherein the oligonucleotide ligands become bound to the target molecule;d) applying magnetic force to isolate one or more solid supports bound to magnetic beads; ande) identifying oligonucleotides attached to said one or more isolated solid supports.