Patent ID: 8841122
Filing Date: 2014-09-23
Classification: C12M

Abstract:
1. A closed cell expansion system, comprising: a cell growth chamber, wherein the cell growth chamber comprises two frustoconical shaped sections joined together to form the cell growth chamber, wherein the two frustoconical shaped sections are joined together at a maximum cross-sectional area of the cell growth chamber to form an interior for growing and suspending cells, wherein walls of the interior taper from the maximum cross-sectional area of the cell growth chamber in opposite directions toward an inlet and an outlet of the cell growth chamber, wherein the inlet and the outlet are disposed on opposite ends of the cell growth chamber, wherein the walls of the interior taper to help keep the cells suspended within the cell growth chamber, and wherein the interior of the cell growth chamber comprises a first volume; a mass transfer device, wherein a plurality of hollow fibers are disposed within a housing of the mass transfer device, the housing comprising an intracapillary portion and an extracapillary portion, and wherein the intracapillary portion comprises a second volume, the second volume being different from the first volume; and a first fluid circulation loop fluidly associated with the cell growth chamber and the mass transfer device, wherein non-adherent cells expand in at least two of the cell growth chamber, the mass transfer device, and the first fluid circulation loop, and wherein the non-adherent cells expand in a media that travels through the cell growth chamber, the mass transfer device, and the first fluid circulation loop.