Patent ID: 6462258
Filing Date: 2002-10-08
Classification: A01N,C12N,Y02A

Abstract:
A DNA construct comprising: a first expression cassette, a second expression cassette, and a third expression cassette, wherein said first expression cassette in operable linkage comprises (i) a Figwort mosaic virus 35S promoter; (ii) a heterologous 5â€² untranslated leader; (iii) a chloroplast transit peptide DNA sequence; (iv) a DNA sequence encoding a glyphosate tolerant EPSPS; and (v) a transcriptional terminator; and said second expression cassette comprising in operable linkage (a) an Arabidopsis Elongation factor 1&agr; promoter with homologous EF1&agr; intron; (b) a chloroplast transit peptide DNA sequence; (d) a DNA sequence encoding a glyphosate tolerant EPSPS; and (e) a transcriptional terminator; and said third expression cassette comprising in operable linkage (I) an Arabidopsis Act2 promoter with homologous Act2 intron; (II) a chloroplast transit peptide DNA sequence; (III) a DNA sequence encoding a glyphosate tolerant EPSPS; and (IV) a transcriptional terminator.