Patent ID: 9168287
Filing Date: 2015-10-27
Classification: A61K,A61L,A61P

Abstract:
1. A substantially cell-free activated leukocyte-conditioned supernatant (ALCS), wherein the ALCS is made by: incubating human leukocytes under conditions of time and temperature to activate the leukocytes, wherein the activated leukocytes comprise activated granulocytes, activated monocytes, and activated lymphocytes; subjecting the activated leukocytes to hypo-osmotic shock; adding to the activated leukocytes subjected to the hypo-osmotic shock to a physiologically acceptable solution containing inorganic ions, organic osmolytes, or a combination thereof in an amount which restores isotonicity; separating the activated leukocytes from the resulting isotonic solution; mixing the separated activated leukocytes with serum to form an incubation composition; incubating the incubation composition ii) at room temperature for 8 to 72 hours; or (ii) at about 37° C. for 3 to 5 hours; and separating the activated leukocytes and other cellular matter from the incubation composition to produce a substantially cell-free ALCS.