Patent ID: 8716022
Filing Date: 2014-05-06
Classification: C12N

Abstract:
1. A plant cell comprising a heterologous nucleic acid encoding a protein, wherein the protein includes the five conserved motifs of a SPO11 protein and wherein the five conserved motifs further include: arginine at a position corresponding to arginine 99 of SEQ ID NO: 40; tyrosine at a position corresponding to tyrosine 103 of (SEQ ID NO: 40); arginine at a position corresponding to arginine 130 of (SEQ ID NO: 40); glycine at a position corresponding to glycine 141 of (SEQ ID NO: 40); glutamate at a position corresponding to glutamate 189 of (SEQ ID NO: 40); leucine at a position corresponding to leucine 197 of (SEQ ID NO: 40); glycine at a position corresponding to glycine 215 of (SEQ ID NO: 40); proline at a position corresponding to proline 217 of (SEQ ID NO: 40); threonine at a position corresponding to threonine 221 of (SEQ ID NO: 40); arginine at a position corresponding to arginine 222 of (SEQ ID NO: 40); aspartate at a position corresponding to aspartate 241 of (SEQ ID NO: 40); proline at a position corresponding to proline 244 of (SEQ ID NO: 40); glycine at a position corresponding to glycine 246 of (SEQ ID NO: 40); and isoleucine at a position corresponding to isoleucine 249 of (SEQ ID NO: 40), wherein said nucleic acid is operably linked to a promoter, and wherein expression of the protein in the cell increases the frequency of homologous non-sister chromatid exchange during meiosis.