Patent ID: 8679052
Filing Date: 2014-03-25
Classification: A61B,G01N

Abstract:
1. A method for accurately determining the diffusible or free concentration of an agent in a medium in which the properties of the probe may change, comprising: a) providing a probe, for which the window volume V b) putting said probe in contact with said medium; c) perfusing a known quantity of a dialysate, for which the diffusion coefficient D of the diffusible agent is known, into the relatively highly permeable section of the probe at a specified flow rate Q; d) allowing said known quantity of dialysate to remain stationary for a specified resting time t e) flushing out said known quantity of dialysate with a single pulse to collect a sample of dialysate of a known volume V f) determining the concentration of said diffusible agent in said dialysate; g) calculating the apparent recovery F h) repeating steps (c) through (g) with the same flow rate and sample volume but at least one different resting time; i) determining the value of λ, f j) calculating the free concentration in the donor as C