Patent ID: 6335201
Filing Date: 2002-01-01
Classification: C12M,C12Q,G01N

Abstract:
A method for measuring the activity of different intracellular chemical reactions in a cell, a portion of a cell, or a group of cells comprising:providing substrate molecules containing a label, the labeled substrate molecules corresponding to a chemical reaction whose activity is to be measured; disposing said substrate molecules within said cell, portion of said cell, or said group of cells; allowing said substrate molecules within the cell, portion of said cell, or said group of cells to take part in the chemical reaction to produce altered substrate molecules; liberating said substrate molecules and said altered substrate molecules from the cell, portion of said cell, or said group of cells; detecting the label to identify the substrate molecules and/or the altered substrate molecules from the cell, portion of said cell, or said group of cells; determining activity of said chemical reaction from a comparison of detected altered substrate molecules with detected substrate molecules, and simultaneously performing each of said steps with a plurality of different substrate molecules, each reporting on different chemical reactions within said call, portion of said cell, or said group of cells.