Patent ID: 6360589
Filing Date: 2002-03-26
Classification: B01J,G01N,Y10T

Abstract:
A liquid chromatograph for analyzing a specimen containing protein, comprising:a separation column (16, 43) for separating a specimen supplied thereto together with a solvent by a pump (11, 45A) for analysis, said specimen containing protein molecules; flow-path control means (14, 42) disposed between said pump (11, 45A) and said separation column (16, 43), said flow-path control means being supplied with said solvent from said pump and said specimen from a specimen injection tube (22, 41), said flow-path control means supplying said solvent to said separation column together with said specimen; detection means (17, 44) supplied with said specimen separated by said separation column (16, 43), said detection means analyzing said specimen; a specimen holding mechanism (21, 41) holding a plurality of vessels (21a) each containing a specimen to be analyzed; specimen injection means (22, 41) sampling a specimen selectively from one of said plurality of vessels (21a), said specimen injection means supplying said sampled specimen to said flow-path control means (14, 42); and a pre-focusing column (30, 40) provided between said flow-path control means (14, 42) and said separation column (16, 43), said pre-focusing column condensing said specimen that is supplied together with said solvent excluding said protein molecules; said pre-focusing column (30, 40) having a volume of less than 2.0 ml, said pre-focusing column (30, 40) being packed with a column packing material of a porous support coated by a silicone polymer having a Siâ€”Râ€”X bond (R represents a spacer part, X represents a sulfonic group) and a Siâ€”Râ€² bond (Râ€² represents a hydrophilic group).