Patent ID: 6828421
Filing Date: 2004-12-07
Classification: G01N

Abstract:
A method for selecting modulators of an interaction between a first protein and a second protein,(a) said first protein being selected from the group consisting of (i) VAP-A (SEQ ID NO:1), (ii) a VAP-A homologue having an amino acid sequence at least 90% identity to that of VAP-A (SEQ ID NO:1) and capable of interacting with a protein selected from the group consisting of PPP1R3 (SEQ ID NO:2) and GTR3 (SEQ ID NO:3), (iii) a VAP-A fragment of VAP-A (SEQ ID NO:1) capable of interacting with a protein selected from the group consisting of PPP1R3 (SEQ ID NO:2) and GTR3 (SEQ ID NO:3), and (iv) a fusion protein containing VAP-A (SEQ ID NO:1), said VAP-A homologue or said VAP-A fragment; and (b) said second protein being selected from the group consisting of (1) PPP1R3 (SEQ ID NO:2) and GTR3 (SEQ ID NO:3), (2) a homologue of a protein selected from the group consisting of PPP1R3 (SEQ ID NO:2) and GTR3 (SEQ ID NO:3) having an amino acid sequence at least 90% identity to that of PPP1R3 (SEQ ID NO:2) or GTR3 (SEQ ID NO:3) and capable of interacting with VAP-A (SEQ ID NO:1), (3) a PPP1R3 fragment of PPP1R3 (SEQ ID NO:2) or a GTR3 fragment of GTR3 (SEQ ID NO:3) and capable of interacting with VAP-A (SEQ ID NO:1), and (4) a fusion protein containing a protein selected from the group consisting of PPP1R3 (SEQ ID NO:2) and GTR3 (SEQ ID NO:3), said protein homologue or said PPP1R3 or GTR3 fragment, said method comprising: contacting said first protein with said second protein in the presence of a test compound; and detecting the interaction between said first protein and said second protein; and selecting a test compound that modulates the interaction between a first protein and a second protein.