Patent ID: 6025351
Filing Date: 2000-02-15
Classification: A61K,C07D

Abstract:
A method for treating blood and other body fluids and tissues which comprises in vitro irradiation of the body fluid or tissue and an effective amount of a Diels Alder adduct or of a metal complex of a Diels Alder adduct, the irradiation being with light of a wavelength at which the Diels Alder adduct or the metal complex has an absorbance peak, wherein the Diels Alder adduct has the structure of Formula 9 or of Formula 10, and the metal complex of a Diels Alder adduct has the structure of Formula 3 or of Formula 4: ##STR21## where M comprises a metal cation that is complexed with two of the nitrogens of the adduct and is Ag, Al, Ce, Co, Cr, Dy, Er, Eu, Ga, Gd, Hf, Ho, In, La, Lu, Mn, Mo, Nd, Pb, Pd, Pr, Pt, Rh, Sb, Sc, Sm, Sn, Tb, Tc-99m, Th, Ti, Tl, Tm, U, V, Y, Yb, Zn or Zr,R1, R2, R3 and R4 can be the same or different, and each is methyl, ethyl, or a monoclonal antibody moiety which is attached to the adduct moiety through a carbonyl which is a part of an amide produced by reaction between an amine function of a monoclonal antibody and a CO.sub.2 R', CH.sub.2 CO.sub.2 R' or CH.sub.2 CH.sub.2 CO.sub.2 R'group of the adduct, and wherein the moiety is of a monoclonal antibody which selectively binds to malignant tumors.R5, R6 and R7 can be the same or different, and each is ethyl, or a monoclonal antibody moiety which is attached to the adduct moiety through a carbonyl which is a part of an amide produced by reaction between an amine function of a monoclonal antibody and a CO.sub.2 R', CH.sub.2 CO.sub.2 R' or CH.sub.2 CH.sub.2 CO.sub.2 R'group of the adduct, and wherein the moiety is of a monoclonal antibody which selectively binds to malignant tumors and R8 is an alkyl group other than t-butyl having from one to four carbon atoms, with the proviso that at least one of R1 through R7 is a monoclonal antibody.