Patent ID: 9127078
Filing Date: 2015-09-08
Classification: C07K,C12N,C12Q,G01N

Abstract:
1. A screening method for identifying a compound that causes an epithelial to mesenchymal transition (EMT) in a normal mammalian cell comprising: (a) adding a test compound to an in vitro biological sample obtained from a mammalian subject, which consists of normal healthy mammalian cells; (b) contacting said biological sample containing the test compound with reagents that can measure or detect the expression level of alternative splice variants of each of genes SLC37A2, FLNB, RALGPS2, ENAH, FNIP1, ARFGAP2, SLK, SCRIB and ARHGEF11 or the proteins encoded thereby; (c) performing an assay to measure the levels of expression of the splice variants; and (d) identifying a test compound as causing an EMT in the sample when wherein the splice variant of: