Patent ID: 8871442
Filing Date: 2014-10-28
Classification: G01N

Abstract:
1. A method for detecting a target in a sample by proximally depositing a detectable moiety, comprising: contacting the sample with a recognition solution, the recognition solution including a specific binding moiety specific to the target; labeling the specific binding moiety with an enzyme; contacting the sample with a detection solution, the detection solution comprising an enzymatic substrate so that the detectable moiety deposits proximally to the target in the presence of a deposition enhancer having a formula wherein R 1 , R 2 , R 3 , and R 4 are independently selected from aliphatic, aryl, halogen, a heteroatom-containing moiety, and hydrogen; R 1 and/or R 3 can be bound to R 2 to form a fused, aromatic ring system; R 5 is a heteroatom-containing moiety; A is selected from a carbon atom, a heteroatom other than sulfur, and any combination thereof; n is 1-5; and detecting the detectable moiety.