Patent ID: 7582606
Filing Date: 2009-09-01
Classification: C07K,C12N

Abstract:
1. A purified recombinantly expressed polypeptide comprising an NADPH oxidase cytosolic cofactor p67 phox having a C-terminal SH3 domain insertion mutation of at least 3 consecutive amino acids positioned between Pro-Glu-Asp-Leu (SEQ ID NO: 35) and Gly-Ile-Phe-Pro-Lys (SEQ ID NO: 34) motifs of said SH3 domain so as to reduce activity of said cofactor relative to a wild-type cofactor of SEQ ID NO: 5 for a p67 phox mutant, wherein said insertion mutation has a net hydropathic index of between −2.4 and 1.6; and an excision, addition or deletion mutation within bases 199-213, which is an activation domain of said cofactor to further reduce activity of said cofactor.