Patent ID: 9005929
Filing Date: 2015-04-14
Classification: C12Q

Abstract:
1. A method for obtaining nucleic acid sequence information, said method comprising the steps of: (a) providing a first sequencing reagent to a target nucleic acid, wherein said first sequencing reagent comprises at least two different nucleotide monomers; (b) detecting the incorporation of a nucleotide monomer present in said first sequencing reagent into a polynucleotide strand complementary to at least a portion of said target nucleic acid; (c) providing a second sequencing reagent to said target nucleic acid, wherein said second sequencing reagent comprises one or more nucleotide monomers, at least one of said one or more nucleotide monomers being different from the nucleotide monomers present in said first sequencing reagent, and wherein said second sequencing reagent is provided subsequent to providing said first sequencing reagent; (d) detecting the incorporation of a nucleotide monomer present in said second sequencing reagent into the polynucleotide strand; and (e) repeating steps (a) through (d) for said target nucleic acid, thereby obtaining sequence information for at least a portion of said target nucleic acid.