Patent ID: 6846971
Filing Date: 2005-01-25
Classification: A01H,C12N

Abstract:
1. A method of transforming Acacia mangium with a gene of interest comprising the steps of: a) activating b) preculturing an explant of c) co-cultivating said activated d) culturing said infected explants in medium comprising supplemented basic MS to induce callus and adventitious buds; and e) culturing said callus or adventitious buds on a selective medium comprising supplemented basic MS medium; wherein in said supplemented basic MS medium comprises a) thidiazuron, b) indole-3-acetic acid, c) casein enzymatic hydrolysate, d) L-ascorbic acid, e) L-glutamirie, f) L-asparacine, g) L-proline, h) sucrose and l) phytagel or agar.