Patent ID: 7307057
Filing Date: 2007-12-11
Classification: A23K,A61K,A61P,C07K,C12N,C12P,C12R

Abstract:
1. Antibiotic 107891 complex, isolated from Microbispora sp. ATCC PTA-5024, comprising Factor A1 and Factor A2 being a white powder having the following characteristics: (A) Mass spectrum recorded from a 0.2 mg/nil solution in methanol:water 80/20 (v/v) with trifluoracetic acid 0.1% on a Thermofinnigan LCQ deca instrument fitted with an electrospray source, using Thermofinnigan calibration mix under the following electrospray conditions: spray voltage: 4.7 kV; capillary temperature: 220.degree. C.; capillary voltage: 3V; infusion mode 10.mu.1/min, showing two double protonated ions at m/z 1124 and nih 1116, corresponding to the lowest isotope composition of Factor A1 and A2, respectively; (B) Infrared spectrum recorded in KBr with a Bruker FT-IR spectophotometer model IFS 48, exhibiting absorption maxima at (cm C) U.V. spectrum perfommed in methanol:H 2080:20 (v/v) with a Perkin-Elmer spectrophotometer Lambda 16, exhibiting two shoulders at 226 and 267 nm; D) E) F) the acid hydrolysate in 6N HCl, (105 degrees C., 24 h) showing the presence of the following amino acids, along with other unidentified peaks, after derivatization with 6-aminoquinolyl-N-hydroxysuccinmmidyl carbamate: lanthionine, methyllanthionine, glycine, proline, valine, aspartic acid (hydrolysis product of asparagine), phenylalanine and leucine; G) the acid hydrolysate in 4N methanesulfonic acid containing 0.2% (w/v) 3-(2-aminoethyl) indole as catalyst (115 degrees C., 16 h) showing the presence of 5-chlorotryptophan; and H) a basic ionizable function detected by acid/base titration performed with 0.01 N potassium hydroxide in 2-methoxyethanol (MCS):H