Patent ID: 6727083
Filing Date: 2004-04-27
Classification: C12N,C12P

Abstract:
An isolated DNA molecule encoding a protein comprising an amino acid sequence selected from the group consisting of:(a) an amino acid sequence represented by SEQ ID NO:1; (b) an amino acid sequence represented by SEQ ID NO:1, which contains a single amino acid substitution therein, wherein said protein converts acetophenone to an optically active 1-phenylehtylamine in the presence of a racemic mixture of sec-butylamine; (c) an amino acid sequence encoded by nucleotides 1 to 1017 of the nucleotide sequence represented by SEQ ID NO:2; (d) an amino acid sequence having 90% or greater amino acid identity with the amino acid sequence represented by SEQ ID NO:1 wherein said protein converts acetophenone to an optically active 1-phenylethylamine in the presence of a racemic mixture of sec-butylamine; (e) an amino acid sequence having 90% or greater amino acid sequence identity with the amino acid sequence represented by SEQ ID NO:1, wherein said protein converts acetophenone to an optically active 1-phenylethylamine in the presence of a racemic mixture of sec-butylamine and has a molecular weight of about 37 kDa as a monomer; and (f) an amino acid sequence having 90% or greater amino acid identity with the amino acid sequence represented by SEQ ID NO:1, wherein said protein converts acetophenone to an optically active 1-phenylehtylamine in the presence of a racemic mixture of sec-butylamine, and wherein said protein is obtainable from a Mycobacterium.