Patent ID: 9206469
Filing Date: 2015-12-08
Classification: C12N,C12Q

Abstract:
1. A method for purification of RNA comprising: (a) contacting a nucleic acid containing sample, which comprises RNA, with a denaturing solvent comprising a chaotropic salt and at least 20% phenol; (b) adding a binding agent to the sample, wherein the binding agent comprises a chaotropic salt, or a lower alcohol or a mixture thereof; (c) contacting the sample with a silica substrate, in the presence of at least 10% phenol, thereby binding RNA to the silica substrate; (d) washing the silica substrate and bound RNA with a wash solution; and (e) eluting the RNA from the silica substrate with an elution buffer, thereby providing purified RNA; wherein, after the addition of the binding agent, the sample does not comprise separate aqueous and organic phases prior to binding the RNA to the silica substrate and wherein: (i) the sample comprises whole blood or blood cells; (ii) the RNA comprises RNA molecules less than 200 nucleotides in length or (iii) the silica substrate comprises magnetic silica beads and wherein one or more of steps (a)-(c) is automated.