Patent ID: 9116150
Filing Date: 2015-08-25
Classification: B01D,G01N

Abstract:
1. A method of packing a chromatography column comprising the steps of: providing a chromatography column having two or more vibration devices attached to its outer surface, and providing a controlled pore glass media with a Protein A ligand and a liquid buffer solution; forming a slurry with the media and the buffer solution, wherein the buffer solution is present from about 10 to about 90% of the volume of the slurry, adding the slurry to the column, allowing the slurry to settle under gravity, activating the vibration devices to generate vibrations with frequencies in the 6000-30000 Hz one or more times to vibrate the slurry in the column at periodic intervals, wherein the vibration devices vibrate from about 30 seconds to about 3 minutes followed by no vibration for a period of about 1 minute to about 5 minutes, and removing the liquid of the slurry during the periodic vibration steps to form a packed bed of media, wherein the packed bed has a lower Height Equivalent to a Theoretical Plate (HETP) compared to a column having only one vibration device.