Patent ID: 8947510
Filing Date: 2015-02-03
Classification: G01N,G06T

Abstract:
1. A method for 3D imaging of a biologic object in an optical tomography system comprising: (a) labeling a subcellular structure of the biological object by introducing at least one nanoparticle-biomarker; (b) positioning the labeled biological object relatively to a microscope objective to present varying angles of view; (c) illuminating the labeled biological object with radiation having wavelengths between 150 nm and 900 nm so that the at least one nanoparticle-biomarker is selected to emit or absorb radiation having wavelengths between 150 and 900 nm, and wherein illuminating employs plasmon resonance energy transfer (PRET), wherein the employment of PRET comprises: (d) filtering a portion of the produced radiation using a set of filters to produce filtered signals within a set of wavelength bands; (e) sensing the filtered signals transmitted through the labeled biological object and the microscope objective within the set of wavelength bands to produce image data; (f) repeating steps (b) through (e) until a set of image data from varying angles of view has been produced; and (g) forming a plurality of cross-sectional images of the biological object from the set of image data; and (h) reconstructing the plurality of cross-sectional images to form a 3D image of the labeled biological object.