Patent ID: 6492139
Filing Date: 2002-12-10
Classification: C07K

Abstract:
Isolated nucleic acid which hybridizes to either a) SEQ ID NO:1 or b) the complement thereof, wherein said nucleic acid encodes vertebrate Smoothened which directly binds to Patched, and wherein said hybridization occurs under the following conditions: 42Â° C. in 50% formamide, 5Ã—SSC, 10Ã—Denhardt's, 0.05M sodium phosphate, (pH 6.5), 0.1% sodium pyrophosphate, 50 &mgr;g/ml sonicated salmon sperm, followed by rinsing in 2Ã—SSC and washing with 0.5Ã—SSC and 0.1% SDS at 42Â° C.