Patent ID: 8852922
Filing Date: 2014-10-07
Classification: A61M,B01J,B01L,B33Y,C12Q,C40B,G01N,Y10T

Abstract:
1. A method of performing a cartridge based assay, said cartridge comprising: (a) a sample chamber having a sample chamber vent port, said sample chamber connected to a sample conduit comprising a first dry reagent, a first waste chamber connected through a first waste chamber conduit to said sample conduit, a first waste chamber vent port connected to said waste chamber; (b) a first reagent chamber containing a volume of a first liquid reagent; (c) a first reagent chamber vent port connected to said first reagent chamber; (d) a first detection chamber connected to said sample chamber by said sample conduit and to said first reagent chamber by a first reagent chamber conduit; (e) an air vent port connected to said sample conduit or said first reagent conduit; said method comprising: (i) moving said sample from said sample chamber into said first sample conduit by opening the sample vent port and applying a vacuum to the first waste chamber vent port; (ii) reconstituting said first dry reagent in said sample; (iii) moving a first slug of said sample having a predetermined volume into said first detection chamber by opening the air vent port and applying the vacuum to the first waste chamber vent port; (iv) moving said sample in said first detection chamber into said first waste chamber; (v) moving said first liquid reagent into said first detection chamber; and (vi) measuring a signal from said first detection chamber.