Patent ID: 9074252
Filing Date: 2015-07-07
Classification: C12Q,G01N

Abstract:
1. A method of detecting a target polynucleotide, comprising: a) contacting a coded molecule with a target polynucleotide, the target polynucleotide including adjacent first and second regions, wherein the coded molecule comprises: b) hybridizing a ligation probe to the target polynucleotide, wherein the ligation probe hybridizes to the first region and the target probe hybridizes to the second region of the target polynucleotide such that a terminus of the ligation probe and a terminus of the target probe are adjacent; c) ligating the terminus of the ligation probe and the terminus of the target probe with a ligase; and d) translocating the coded molecule through a nanopore and detecting a signal pattern associated with the coded molecule.