Patent ID: 6218157
Filing Date: 2001-04-17
Classification: A61K,C12N,C12P

Abstract:
A method for producing an (S)-4-halo-3-hydroxybutyric acid ester, the method comprising reacting a 4-haloacetoacetic acid ester with a purified enzyme to form a reaction mixture, and recovering the (S)-4-halo-3-hydroxybutyric acid ester from the reaction mixture, the purified enzyme having the following properties:in the presence of .beta.-nicotinamide adenine dinucleotide phosphate (NADPH) as an electron donor, reduces a 4-haloacetoacetic acid ester to produce a (S)-4-halo-3-hydroxybutyric acid ester;does not dehydrogenate a (S)-4-halo-3-hydroxybutyric acid ester substrate; andexhibits a molecular weight of about 190,000 daltons when measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.