Patent ID: 8178327
Filing Date: 2012-05-15
Classification: B01D,C12N,C12P,C12Y

Abstract:
1. A non-naturally occurring microbial organism having nucleic acids encoding enzymes of a 1,4-butanediol (BDO) pathway, said microbial organism comprising at least one exogenous nucleic acid encoding a BDO pathway enzyme expressed in a sufficient amount to produce BDO, wherein said BDO pathway enzymes comprise glutamate dehydrogenase, said enzyme classified as EC 1.4.1.a and converting alpha-ketoglutarate to glutamate; glutamate decarboxylase, said enzyme classified as EC 4.1.1.a and converting glutamate to 4-aminobutyrate; 4-aminobutyrate oxidoreductase (deaminating), said enzyme classified as EC 1.4.1.a and converting 4-aminobutyrate to succinate semialdehyde; and 4-hydroxybutyrate dehydrogenase, said enzyme classified as EC 1.1.1.a and converting succinate semialdehyde to 4-hydroxybutyrate; and wherein said BDO pathway enzymes further comprise: (a) 4-hydroxybutyrate kinase, said enzyme classified as EC 2.7.2.a and converting 4-hydroxybutyrate to 4-hydroxybutyryl-phosphate; phosphotrans-4-hydroxybutyrylase, said enzyme classified as EC 2.3.1.a and converting 4-hydroxybutyryl-phosphate to 4-hydroxybutyryl-CoA; 4-hydroxybutyryl-CoA reductase, said enzyme classified as EC 1.2.1.b and converting 4-hydroxybutyryl-CoA to 4-hydroxybutanal; and 1,4-butanediol dehydrogenase, said enzyme classified as EC 1.1.1.a converting 4-hydroxybutanal to 1,4-butanediol; (b) 4-hydroxybutyryl-CoA transferase, said enzyme classified as EC 2.8.3.a and converting 4-hydroxybutyrate to 4-hydroxybutyryl-CoA, 4-hydroxybutyryl-CoA hydrolase, said enzyme classified as EC 3.1.2.a and converting 4-hydroxybutyrate to 4-hydroxybutyryl-CoA, or 4-hydroxybutyryl-CoA ligase, said enzyme classified as EC 6.2.1.a and converting 4-hydroxybutyrate to 4-hydroxybutyryl-CoA; 4-hydroxybutyryl-CoA reductase; and 1,4-butanediol dehydrogenase; (c) 4-hydroxybutyryl-CoA transferase, 4-hydroxybutyryl-CoA hydrolase, or 4-hydroxybutyryl-CoA ligase; and 4-hydroxybutyryl-CoA reductase (alcohol forming), said enzyme classified as EC 1.1.1.c and converting 4-hydroxybutyryl-CoA to 1,4-butanediol; (d) 4-hydroxybutyrate kinase; 4-hydroxybutanal dehydrogenase (phosphorylating), said enzyme classified as EC 1.2.1.d and converting 4-hydroxybutyryl-phosphate to 4-hydroxybutanal; and 1,4-butanediol dehydrogenase; or (e) 4-hydroxybutyrate kinase; phosphotrans-4-hydroxybutyrylase; and 4-hydroxybutyryl-CoA reductase (alcohol forming).