Patent ID: 7645594
Filing Date: 2010-01-12
Classification: C12Q,G01N,Y10T

Abstract:
1. A method of preparing an assay sample for detecting bacteria by a flow cytometer, comprising: providing a diluent comprising a cationic surfactant, a buffer for maintaining a pH of 2.0-4.5 and an effective amount of a substance capable of reducing nitrite ions and a staining solution comprising a polymethine dye for staining bacteria; mixing a urine sample with the diluent; and preparing the assay sample by mixing the mixture of the urine sample and the diluent with the staining solution; wherein the polymethine dye is at least one selected from the following group consisting of: wherein R (11) a compound represented by the following general formula: wherein R wherein the cationic surfactant is a quaternary ammonium salt represented by the following formula: wherein R