Patent ID: 9176125
Filing Date: 2015-11-03
Classification: G01N

Abstract:
1. A kit for use in a method of indirectly coupling a ligand to a polypeptide to be labelled with the ligand; the kit comprising a freeze-dried aliquot comprising both (a) an isolated scaffold molecule which is covalently attached to at least one said ligand and a plurality of thiol-reactive groups reactive towards a thiol group to be formed in situ on the polypeptide to be labelled; and (b) a thiol-generator comprising 2-iminothiolane, for in situ creation of thiol groups on the polypeptide to be labelled wherein said ligand comprises a molecule selected from the group consisting of biotin, avidin, fluorescent dye, chromophore, biotin attached with N-hydroxysuccinimide, biotin attached with maleimide, avidin attached with N-hydroxysuccinimide, and avidin attached with maleimide.