Patent ID: 9173964
Filing Date: 2015-11-03
Classification: A61K,A61P,C12N

Abstract:
1. An isolated nucleic acid construct, comprising: a) a first open reading frame encoding a diphtheria toxin, the first open reading frame being operably linked to an H19 promoter; and b) a second open reading frame encoding a diphtheria toxin, the second open reading frame being operably linked to an IGF-II-promoter selected from IGF-II P3 and IGF-II P4 promoter sequences; and wherein the diphtheria toxin is diphtheria toxin A (DTA) or comprises a sequence that is at least 90% homologous to the sequence set forth in SEQ ID NO: 7; and wherein the nucleic acid sequence of the H19 promoter exhibits H19 promoter activity and is at least 90% homologous to SEQ ID NOS: 1 or 2, and the nucleic acid sequence of the IGF-II P4 promoter exhibits IGF-II P4 promoter activity and is at least 90% homologous to SEQ ID NO: 9, the nucleic acid sequence of the IGF-II P3 promoter exhibits IGF-II P3 promoter activity and is at least 90% homologous to the sequence selected from SEQ ID NO: 8, SEQ ID NO: 12, and SEQ ID NO: 17.