Patent ID: 6512092
Filing Date: 2003-01-28
Classification: A61K,C07K

Abstract:
A process of coupling an amino acid residue to a peptide chain which comprises:(i) providing an amino acid residue having a free carboxylic group and blocked amino group, optionally having additional blocked finctional side chains; (ii) reacting the blocked amino acid with bis-(trichloromethyl)carbonate in a solvent inert to this reaction to obtain an amino acid chloride; (iii) neutralizing the free acid with an organic base; (iv) adding the resulting suspension containing the amino acid chloride to a compound selected from the group consisting of a peptide having a blocked carboxyl terminus and a free amino terminus, and a peptidyl resin having at least one free amino terminus; and (v) providing reaction conditions enabling the coupling of the amino acid chloride to the peptide to yield a peptide elongated by one amino acid residue.