Patent ID: 6270962
Filing Date: 2001-08-07
Classification: C12Q

Abstract:
A method of decreasing the incidence of DNA polymerase stops occurring in a reaction mixture containing a DNA polymerase comprising adding to the reaction mixture an amount of a composition of the formula: ##STR5##wherein:R.sup.1, R.sup.2, and R.sup.3 may be the same or different and are independently selected from the group consisting of hydrogen, methyl, ethyl, and propyl, with the proviso that no more than two of R.sup.1, R.sup.2, and R.sup.3 are hydrogen; andX is a moiety selected from the group consisting of:radicals of the formulas(a) .dbd.O; and ##STR6##wherein:R.sup.4 is selected from the group consisting of methyl and hydrogen and, when combined with R.sup.1, forms a pyrrolidine ring;R.sup.5 is selected from the group consisting of --CO.sub.2 H and --SO.sub.3 H; andn is an integer of from 0 to 2;with the proviso that, when R.sup.1 and R.sup.4 form a pyrrolidine ring, no more than one of R.sup.2 and R.sup.3 is hydrogen, and wherein the composition is added in an amount effective to decrease the incidence of DNA polymerase stops.