Patent ID: 9220733
Filing Date: 2015-12-29
Classification: A61K,A61L,C12N

Abstract:
1. A method of removing immunogenic antigens from a tissue, comprising: a) solubilizing water-soluble antigens in the tissue by contacting the tissue with a first solution comprising a first buffering agent, a first reducing agent, a first protease inhibitor, and one or more salts suitable for maintaining protein solubility, wherein the first solution is substantially free of an amphiphile, such that the water-soluble antigens are dissolved in the first solution; b) separating the tissue from the solubilized water-soluble antigens in the first solution; c) solubilizing lipid-soluble antigens in the tissue by contacting the tissue with a second solution comprising a second buffering agent, a second reducing agent, a second protease inhibitor, one or more salts suitable for maintaining protein solubility and an amphiphile, such that the lipid-soluble antigens are dissolved in the second solution; and d) separating the tissue from the solubilized lipid-soluble antigens in the second solution; thereby removing immunogenic antigens from the tissue.