Patent ID: 7473821
Filing Date: 2009-01-06
Classification: A01N,C07K,C12N,Y02A

Abstract:
1. An isolated nucleic acid molecule comprising a nucleotide sequence encoding Bacillus thuringiensis Cry8Bb1 protoxin, wherein said Cry8Bb1 protoxin has at least one proteolytic activation site that has been engineered to comprise a cleavage site that is sensitive to an insect gut protease, wherein said proteolytic activation site comprises an amino acid sequence selected from the group consisting of LXQS (SEQ ID NO:1), LSQS (SEQ ID NO:2), LXQSLXQS (SEQ ID NO:3), and LSQSLSQS (SEQ ID NO:4), and wherein cleavage of said Cry8Bb1 protoxin by said insect gut protease produces an active Cry8Bb1 toxin or a variant thereof, wherein said variant has pesticidal activity and has at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO:11.