Patent ID: 8911992
Filing Date: 2014-12-16
Classification: C07K,C12N

Abstract:
1. A method of removing genomic DNA from a cell culture biological sample or culture supernatant biological sample comprising a protein target molecule and positively charged complexes comprising genomic DNA, the method comprising, contacting the cell culture biological sample or culture supernatant biological sample to a cation exchange ligand comprising as a charged substituent a sulfonic acid or phosphoric acid moiety, said ligand bound to a solid support or bound to a soluble polymer under conditions such that: (a) positively charged genomic complexes comprising DNA from the sample bind the cation exchange ligand; and (b) non-binding components of the sample, including the protein target molecule, do not substantially bind to the cation exchange ligand, the solid support, or the soluble polymer; separating the non-binding components of the sample, including the target molecule, from the positively-charged complexes bound to the cation exchange ligand to produce a purified sample having a reduced amount of genomic DNA; and performing at least one more protein target molecule purification step on the purified sample having a reduced amount of genomic DNA, wherein the protein target does not bind the cation exchange ligand from the contacting through the separating.