Patent ID: 6451372
Filing Date: 2002-09-17
Classification: C12Q

Abstract:
A method of manufacturing a biosensor which comprises an electrically insulating base plate, an electrode system including a measuring electrode and a counter electrode formed on the base plate, and a reaction reagent system including at least an oxidoreductase and an electron mediator as reagents, wherein the reagents of the reaction reagent system are present as a reaction layer structure that comprises at least one reaction layer,said method comprising a step of forming a specific reaction layer of the reaction layer structure, the specific layer comprising at least one of the reagents of the reaction reagent system, said step comprising: (1) dissolving the at least one of the reagents in a solvent of a sublimable substance to prepare a solution; (2) applying the solution on at least one of the base plate, the measuring electrode, and the counter electrode; (3) freezing the applied solution; and (4) sublimating the solvent included in a solid matter of the frozen solution under reduced pressure for removal, thereby forming the specific reaction layer on at least one of the base plate, the measuring electrode, and the counter electrode.