Patent ID: 9081020
Filing Date: 2015-07-14
Classification: C07K,G01N

Abstract:
1. A method for selecting a G-protein coupled receptor (GPCR) with increased stability, the method comprising: a) providing one or more mutants of a parent GPCR in a membrane-containing composition, b) exposing the one or more mutants of a parent GPCR in the membrane-containing composition to an amount of a membrane destabilising agent which is effective to destabilise the parent GPCR in-situ without solubilising the GPCR, c) determining whether the or each mutant GPCR has increased stability with respect to its structure or a biological activity compared to the stability of the parent GPCR with respect to its structure or the same biological activity, and d) selecting the one or more mutants which have increased stability compared to the stability of the parent GPCR.