Patent ID: 6613530
Filing Date: 2003-09-02
Classification: C07K,C12N,G01N,Y10S

Abstract:
A method for detection of an antibody against HIV or HCV in a liquid sample, the method comprisinga) incubating (1) said sample, (2) a solid phase, (3) a first antigen for said antibody, wherein the first antigen has at least one marker group, and (4) a second antigen for said antibody, wherein the second antigen binds to the solid phase, under conditions to obtain an immune complex comprising a solid phase-bound second antigen to which is bound the antibody and to which is bound the first antigen; and b) detecting said antibody by direct or indirect detection of the marker group; and wherein at least one of said antigen is of formula (Ia) or (Ib) (P-)nT(-L)mâ€ƒâ€ƒ(Ia) T(-P-Lm)nâ€ƒâ€ƒ(Ib) â€ƒwherein T is a carrier, P is a peptide comprising an epitope region and a spacer region wherein said epitope region is reactive with the antibody and said spacer region comprises a peptide sequence of a length from 1 to 10 amino acids which is not reactive with the antibody, L is the marker group or a group which binds to the solid phase, - is a covalent coupling, n is 2-40 and m is 1-10.