Patent ID: 7507796
Filing Date: 2009-03-24
Classification: C07K,G01N

Abstract:
1. A bivalent monomeric F v antibody formed by one single-chain F v monomer having four variable domains, wherein said four variable domains are V H -A, V L -B, V H -B and V L -A, wherein V H -A and V L -A are V H and V L domains of an antibody specific for antigen A, respectively, and V H -B and V L -B are V H and V L domains of an antibody specific for antigen B, respectively; V H -A is linked to V L -B by peptide linker 1, V L -B is linked to V H -B by peptide linker 2 and V H -B is linked to V L -A by peptide linker 3; said peptide linker 1 and said peptide linker 3 are a peptide bond or have 1 to about 10 amino acids; and said peptide linker 2 has about 10 to about 30 amino acids; V H -A and V L -A associate intramolecularly to form a binding site for antigen A; and V L -B and V H -B associate intramolecularly to form a binding site for antigen B.