Patent ID: 8067383
Filing Date: 2011-11-29
Classification: C12N

Abstract:
1. A double-stranded DNA construct comprising: a first structural gene sequence comprising about 20-30 consecutive nucleotides identical in sequence to a region of a target gene encoding a viral DNA polymerase, a viral RNA polymerase, or a viral coat protein in a mammalian cell; a second structural gene sequence comprising about 20-30 consecutive nucleotides identical in sequence to, and in an inverted orientation relative to, the about 20-30 consecutive nucleotides of the first structural gene sequence, such that a repeating sequence which is only about 20-30 consecutive nucleotides in length identical to the region of the target gene is present in the DNA construct; a stuffer fragment which consists of nucleotides other than the nucleotides of the repeating sequence, and which separates and links the first and second structural gene sequences; a promoter operable in the mammalian cell; and a transcription termination sequence active in the mammalian cell, wherein the first structural gene sequence, the stuffer fragment and the second structural gene sequence are all operably connected to the promoter and the transcription termination sequence.