Patent ID: 6618679
Filing Date: 2003-09-09
Classification: C12Q,G16B

Abstract:
A method for analyzing gene expression comprising:a) obtaining a plurality of target sequences, wherein the plurality of target sequences comprises cDNA; b) multiplex amplifying said plurality of target sequences, wherein multiplex amplifying comprises combining the plurality of target sequences, a plurality of target-specific primers, and one or more universal primers, and wherein the universal primer is provided in an excess concentration relative to the target-specific primer, thereby producing a plurality of amplification products; c) separating one or more members of the plurality of amplification products; d) detecting one or more members of the plurality of amplification products, thereby generating a set of gene expression data; e) storing the set of gene expression data in a database; and f) performing a comparative analysis on the set of gene expression data, thereby analyzing the gene expression.