Patent ID: 6610483
Filing Date: 2003-08-26
Classification: C07D,C12Q

Abstract:
A method for identifying a pattern of one or more cellular responses attributable to selective inhibition of a wild-type signaling molecule comprising:(a) providing mutant cells expressing a mutant form of the wild-type signaling molecule, wherein the mutant form of the wild-type signaling molecule retains the function of the wild-type signaling molecule; (b) contacting said mutant cells with an inhibitor, said inhibitor being a selective inhibitor of said mutant form of the wild-type signaling molecule; (c) identifying one or more cellular responses exhibited by said mutant cells before and after contact with said inhibitor; (d) comparing said one or more cellular responses obtained in step (c) to identify a pattern of cellular responses exhibited by said mutant cells after said contact; and, (e) comparing the pattern obtained in step (d) to a pattern of one or more cellular responses obtained from the wild-type signaling molecule, wherein, when said wild-type signaling molecule pattern comprises one or more cellular responses which are characteristic of one or more cellular responses of the pattern of the inhibition of said mutant form of the wild-type signaling molecule, a pattern of one or more cellular responses attributable to selective inhibition of a wild-type signaling molecule is identified.