Patent ID: 7045310
Filing Date: 2006-05-16
Classification: C07F,C07K,G01N,Y10S,Y10T

Abstract:
1. A method for measuring the concentration of an analyte in a sample of a liquid, said method comprising contacting said sample with predetermined amounts of (1) a specific binding partner for said analyte, and (2) a detectable compound of the formula: wherein R and R R R, R Q is O, S, or NR —L— is a divalent linker; k is 1 or 0; i is 1, 2, 3, 4, 5 or 6; B comprises a ligand that binds to the specific binding partner and is selected so that the detectable compound binds competitively with the analyte to said binding partner; d is +2 or +3; X and Y are anions selected from monovalent anions chloride, bromide, iodide, fluoride, tetrafluoroborate, perchlorate, and nitrate and divalent anions sulfate, carbonate or sulfite wherein x and y are independently 0, 1, 2, or 3 so that the net charge of X determining electrochemically the concentration of the detectable compound not bound to the specific binding partner, wherein the concentration of the detectable compound not bound to the specific binding partner is proportional to the concentration of analyte present in the sample of fluid.