Patent ID: 8603741
Filing Date: 2013-12-10
Classification: C07H,C12Q

Abstract:
1. A method for sequencing a nucleic acid template comprising: providing a sequencing mixture comprising a polymerase enzyme, a template nucleic acid, a primer, a polymerase regent solution having the components for carrying out template directed synthesis of a growing nucleic acid strand, a plurality of types of nucleotide analogs; wherein each type of nucleotide analog has a labeled leaving group, each with a different label, that blocks further nucleotide incorporations at the 3′ hydroxyl position of the incorporated nucleotide analog; and a selective cleaving activity which more effectively cleaves a leaving group from an incorporated nucleotide analog than from an unincorporated nucleotide analog; carrying out nucleic acid synthesis such that a plurality of nucleotide analogs are added sequentially whereby: i) a nucleotide analog is incorporated, then ii) the labeled leaving group on that nucleotide analog is cleaved by the selective cleaving activity, making the 3′ hydroxyl group available for a subsequent nucleotide analog addition; and detecting light from the labels while the polymerase reaction is occurring to determine a sequence of the template nucleic acid.