Patent ID: 9037445
Filing Date: 2015-05-19
Classification: G16B

Abstract:
1. A computer-implemented method for achieving an optimal function of a biochemical reaction network in cells in a cell culture, comprising: (a) calculating in a computer one or more optimal cell culture parameters in a cell culture using a flux balance analysis constrained by a solvent capacity of cells in the cell culture, wherein the flux balance analysis is further constrained by one or more cell components other than a metabolic enzyme selected from the group consisting of ribosomal protein, mRNA and DNA which increases in concentration with increasing cell growth rate, and wherein the optimal cell culture parameter is calculated by determining an optimal property of a biochemical reaction network comprising a list of biochemical reactions by applying a computational optimization method to one or more of the biochemical reactions of the biochemical reaction network, the optimization method comprising (i) altering one or more elements of the one or more biochemical reactions in the biochemical reaction network and re-computing the optimal property, and (ii) repeating (i) until an optimal function is reached; and (b) initiating or maintaining the optimal cell culture parameter in the cell culture to achieve the optimal function of the biochemical reaction network in the cells.