Patent ID: 9164079
Filing Date: 2015-10-20
Classification: A61K,A61M,A61P,C12M,G01N

Abstract:
1. An autologous cell concentrating system comprising: a blood separation component having a blood sample input and having a centrifuge to separate the blood sample into a red blood cell fraction, and a plasma fraction, where the plasma fraction comprises a target cell-rich fraction and a target cell-poor fraction, the blood separation component having a volume of between 60 mL and no more than 250 mL, the blood sample being a whole blood sample or a bone marrow sample; a first vessel for storing the target cell-poor fraction separated from the blood sample, the first vessel comprising a syringe opening; a second vessel for storing the target cell-rich fraction separated from the blood sample, the second vessel comprising a syringe opening; a measurement system that measures a total number of target cells in the cell concentrating system; a processor comprising a concentration and flow logic and control component configured to determine a first volume of the target cell-poor fraction to mix with the target cell-rich fraction in order to form a target cell-rich concentrate having a concentration of target cells that is within a target concentration range for studying or defining a dose-response relationship in a patient, the concentration and flow logic and control component further configured to determine whether the target cell-rich concentrate has a concentration of target cells within the target concentration range; a first valve controlled by the processor to remove the target cell-poor fraction to the first vessel and to remove the target cell-rich fraction to the second vessel; and a mix component configured to form the target cell-rich concentrate by mixing the first volume of the target cell-poor fraction with the target cell-rich fraction, the mix component comprising a second valve controlled by the processor to transfer the first volume of the target cell-poor fraction from the first vessel to the second vessel containing the target cell-rich fraction; wherein the processor is configured to control the cell concentrating system to achieve a target concentration range of 1.0-1.5×10