Patent ID: 7860658
Filing Date: 2010-12-28
Classification: A01H

Abstract:
1. A method for mapping traits in an organism comprising: a) providing a population of second division restitution (SDR)-0 organisms that each arise from one member of a population of unreduced SDR-0 cells resulting from second division restitution; b) producing a population of SDR-1 progeny organisms from each SDR-0 organism; c) phenotyping the SDR-1 progeny organisms within each of the SDR-1 progeny populations to identify segregating traits within each SDR-1 progeny population; d) for each SDR-1 progeny organism that exhibits segregating traits in a SDR-1 progeny population, genotyping the corresponding SDR-0 organism; e) genotyping the other SDR-0 organisms in the SDR-0 population which do not correspond to the SDR-1 progeny having the segregating traits, f) comparing the genotype of the SDR-0 organisms in (d) with the genotype of the other SDR-0 organisms in (e); and g) using the SDR-0 genotypes identified in (d)-(f) to identify in each SDR-0 organism heterozygous chromosomal regions associated with the segregating trait identified in the corresponding SDR-1 progeny population in (d).