Patent ID: 8349579
Filing Date: 2013-01-08
Classification: C12Q,G01N

Abstract:
1. A method of detecting matrix metalloproteinase (MMP) activity in a biological tissue sample comprising detecting native full-length and cleaved galectin-3 in said sample by contacting said sample with at least one anti-galectin-3 antibody that binds full-length galectin-3 and at least one antibody that binds cleaved galectin-3, determining the binding of said antibodies in said sample, wherein the presence of cleaved galectin-3 binding to said antibody correlates with the presence of MMP-2 or MMP-9 enzymatic activity in said sample; and confirming the presence of MMP within said sample by contacting said sample with at least one antibody capable of recognizing at least one of MMP-2 and MMP-9 and detecting binding of said antibody to said MMP.