Patent ID: 8410414
Filing Date: 2013-04-02
Classification: G02B

Abstract:
1. A fluorescence microscopy imaging system, for detecting a fluorescence signal of a sample, comprising: a module for detecting fluorescence, having a fluorescence excitation light source generator, an optical assemble module of fluorescence detection, and a fluorescence detector, wherein the fluorescence excitation light source generator is capable of generating an excitation light beam having a first wavelength, the excitation light beam is guided by the optical assemble module of fluorescence detection along a light path to pass through an objective and focused on the sample so as to excite the sample to emit fluorescence light, and then the excited fluorescence light of the sample is guided by the optical assemble module of fluorescence detection along the light path to the fluorescence detector; and a module for focusing control, having a servo light source generator, a dichroic optical element, an optical assemble module of servo focus, an actuator, a servo light beam reflecting film, and a focusing detection device; wherein the servo light source generator is capable of generating a servo light beam having a second wavelength, the servo light beam reflecting film is disposed on an observation plane of the sample, the dichroic optical element is disposed at an area of intersection of the optical assemble module of servo focus and the light path, and is used to couple the servo light beam to the light path, such that the servo light beam to pass through the objective and reach the servo light beam reflecting film, and then guides a return beam signal reflected back from the servo light beam reflecting film along a servo light path to the focusing detection device, the focusing detection device controls the actuator to move along an optical axis of the light path according to the return beam, and the actuator is used to move the objective for focusing.