Patent ID: 6478938
Filing Date: 2002-11-12
Classification: C12Q,G01N

Abstract:
An electrochemical membrane strip biosensor comprising(a) a first membrane strip pad for sample application; (b) a second membrane strip pad, partially superimposed in length with the first membrane strip pad for signal generation, said second membrane strip pad including (i) a binding protein, specifically binding to an analyte, immobilized at a defined area of the second membrane's surface, and (ii) at least one pair of electrodes screen-printed on said second membrane's surface such that the binding protein-immobilized area is located between the two electrodes; (c) a third membrane strip pad, partially superimposed in length with the second membrane strip pad for absorption of an aqueous medium; and (d) a conjugate between (i) a binding protein specifically binding to a different site on the analyte from that recognized by said immobilized binding protein of the second membrane strip, and (ii) a metal colloid having surfaces bound by a conducting polymer, wherein said conjugate is either placed at a predetermined site on the first membrane strip pad in a dry state or is contained in a separate container and placed on said first strip membrane pad prior to use of the biosensor.