Patent ID: 6174666
Filing Date: 2001-01-16
Classification: A61K,A61P,C07K,C12N

Abstract:
A method for reducing the effect of inhibitory/instability sequences within the coding region of a mRNA, wherein said effect of said inhibitory/instability sequences is a post-transcriptional effect, said method comprising the steps of:(a) providing a gene which encodes said mRNA;(b) identifying the inhibitory/instability sequences within said gene which encode said inhibitory/instability sequences within the coding region of said mRNA;(c) mutating said inhibitory/instability sequences within said gene by making multiple point mutations;(d) transfecting said mutated gene into a cell;(e) culturing said cell in a manner to cause expression of said mutated gene;(f) detecting the level of expression of said gene to determine whether the effect of said inhibitory/instability sequences within the coding region of the mRNA has been reduced.