Patent ID: 6340563
Filing Date: 2002-01-22
Classification: C12Q

Abstract:
A method for topographic genotyping consisting of the steps of:placing a fixative treated tissue specimen of intact cells having DNA of a patient under a microscope; inspecting the fixative treated tissue specimen with the microscope for determination of cellular targets for genetic analysis; choosing a target on the fixative treated tissue specimen based on specific morphologic criteria in turn reflecting specific disease related cellular alterations; separating a piece from said chosen target; placing the piece directly into a container containing a lysis buffer after the piece is separated from the target; performing a single centrifugation on the container with the piece to create a pellet in the container without additional extraction or lysis steps to produce supernatent; withdrawing supernatant from the pellet in the container without any further extraction steps to obtain DNA sequences from the target so the DNA sequences can be amplified directly without preparation or additional extraction steps of the supernatent; amplifying the DNA sequences directly from the supernatant without any further preparation of the DNA sequences or extraction of the DNA sequences from the supernatent; and detecting mutations in the DNA sequences.