Patent ID: 6242208
Filing Date: 2001-06-05
Classification: C12Q

Abstract:
A process for the determination of LDH.sub.1 in a sample selected from the group consisting of human serum and human plasma, which comprises inhibiting LDH.sub.2, LDH.sub.3, LDH.sub.4 and LDH.sub.5 in the sample with .alpha.-chymotrypsin in the presence of a protein-denaturating agent and then determining LDH.sub.1 remaining uninhibited, wherein the LDH.sub.1 remaining uninhibited is determined according to a total LDH isozyme assay which comprises catalytically developing a chromogen or dye precursor with the LDH isozyme remaining uninhibited and then measuring absorption in a visible light range or comprises measuring ultraviolet absorption of coenzyme NADH reduced by catalytic effect of the LDH isozyme remaining uninhibited.