Patent ID: 9139863
Filing Date: 2015-09-22
Classification: C07K,C12Q,G01N

Abstract:
1. A conformationally stabilized ubiquitin protein comprising the amino acid sequence of SEQ ID NO: 15, wherein Xaa at the position corresponding to amino acid 7 or 8 of SEQ ID NO: 15 is cysteine and Xaa at the position corresponding to amino acid 69 of SEQ ID NO: 15 is cysteine, wherein a β1/β2 loop region of the stabilized protein exhibits slower conformational dynamics in comparison to wild type ubiquitin protein as measured by NMR R 2 dispersion, and wherein said conformationally stabilized ubiquitin protein exhibits increased binding affinity to a deubiquitinase of the Ubiquitin Specific Protease (USP) family as compared to said wild type ubiquitin protein.