Patent ID: 6951722
Filing Date: 2005-10-04
Classification: C12N,C12P,C12Q

Abstract:
1. A method for amplifying a nucleic acid, characterized in that the method comprises: (a) preparing a reaction mixture by mixing a nucleic acid as a template, a deoxyribonucleotide triphosphate, a DNA polymerase having a strand displacement activity, at least one primer and an endonuclease that cleaves an extended strand generated from the primer, wherein the primer is a chimeric oligonucleotide primer that is substantially complementary to the nucleotide sequence of the nucleic acid as the template and contains a ribonucleotide as well as at least one selected from the group consisting of a deoxyribonucleotide and a nucleotide analog, the ribonucleotide being positioned at the 3′-terminus or on the 3′-terminal side of the primer; and (b) incubating the reaction mixture for a sufficient time to generate a reaction product under conditions where specific annealing of the primer to the nucleic acid as the template, an extended strand synthesis reaction and a strand displacement reaction by the DNA polymerase, and a reaction of cleaving the extended strand by the endonuclease take place.