Patent ID: 8492343
Filing Date: 2013-07-23
Classification: A61P,C12N,C40B

Abstract:
1. A method of preparing a protein scaffold library based on a Kringle domain, the method comprising: providing an amino acid sequence comprising a Kringle domain, which comprises C1, G6, Y9, D10, G11, T16, G19, C22, Q23, W25, P30, H31, H33, G34, K48, N49, Y50, C51, R52, N53, P54, D55, P61, W62, C63, F64, T65, E73, L74, C75, P78, R79, and C80; and introducing artificial mutation in the amino acid sequence at one or more amino acid residues other than amino acid residues of C1, G6, Y9, D10, G11, T16, G19, C22, Q23, W25, P30, H31, H33, G34, K48, N49, Y50, C51, R52, N53, P54, D55, P61, W62, C63, F64, T65, E73, L74, C75, P78, R79, and C80, thereby producing a mutant amino acid sequence, which is part of the protein scaffold library.