Patent ID: 7074569
Filing Date: 2006-07-11
Classification: C07H,C12Q

Abstract:
1. A method for detecting a plurality of selected nucleotide sequences within one or more target nucleic acids, comprising: contacting at least one or more target nucleic acids with a plurality of mobility-modified sequence-specific nucleobase polymers under conditions that distinguish those nucleobase polymers that hybridize to the target nucleic acid, wherein each said nucleobase polymer is a mobility-modified sequence-specific nucleobase polymer comprising a mobility-modifying polymer linked to a sequence-specific nucleobase polymer, according to Structural formula (II) or (III):  or a salt thereof, wherein: R each R R each R each X is independently selected from the group consisting of O, S, NH and NH—C(O); each a is independently an integer from 1 to 6; each b is independently an integer from 0 to 40; each d is independently an integer from 1 to 200; and OLIGO comprises a sequence-specific nucleobase polymer, with the proviso that at least one R wherein each said nucleobase polymer has a distinctive ratio of charge to translational frictional drag; and detecting those nucleobase polymer that have hybridized to the target nucleic acid.