Patent ID: 6297053
Filing Date: 2001-10-02
Classification: C07K,C12N,C12Q,C12Y,C40B

Abstract:
A method for producing a binding protein with a desired property, the method comprising:(i) providing a population of DNA fragments comprising at least one polynucleotide encoding all or part of a binding protein;(ii) recursively recombining the DNA fragments in vitro or in vivo to form a pool of recombined binding protein polynucleotides, which pool of binding protein polynucleotides comprises a library of shuffled DNA sequences wherein recursively recombining the DNA fragments comprises recombining the DNA fragments to form a pool of recombined binding protein polynucleotides; selecting the pool for the desired property, thereby providing selected binding protein polynucleotides; and recombining the selected binding protein polynucleotides and(iii) identifying at least one recombined binding protein polynucleotide encoding a binding protein with a desired property.