Patent ID: 8673563
Filing Date: 2014-03-18
Classification: C12Q

Abstract:
1. An amplification method of CpG-containing nucleic acid derived from a target gene and/or gene locus which is likely be contained in a biological sample, wherein the amplification method comprises a step of amplifying the CpG-containing nucleic acid using a primer set comprising a first primer which can hybridize with both methylated and unmethylated nucleic acids and a second primer which hybridizes specifically with only one of methylated and unmethylated nucleic acids, wherein the primer region of the second primer contains one to eight base additions and/or deletions on at least one end of the 3′ end and 5′ end of the primer region of the first primer or does not contain any additions and deletion on the both ends, wherein the first primer and second primer are introduced together and wherein a concentration ratio of the first primer to the second primer is 10:1 to 1:1.