Patent ID: 6207150
Filing Date: 2001-03-27
Classification: A01K,A61K,A61P,C12N

Abstract:
A nucleic acid coding for a herpes simplex virus type 1 thymidine kinase variant, wherein the variant has a mutation in the region corresponding to the ATP-binding site, and an additional one or more mutations in the N-terminal or C-terminal regions, the mutations selected from the following group or from a mutation resulting in a degenerate coding variant of any in the following group:adenine substituting for the guanine at position 180 (G180A);adenine substituting for the guanine at position 16 (G16A);adenine substituting for the guanine at position 28 (G28A);adenine substituting for the guanine at position 30 (G30A);adenine substituting for the guanine at positions 28 and 30 (G28A and G30A);thymine substituting for the cytosine at position 591 (C591T);thymine substituting for the cytosine at position 892 (C892T);thymine substituting for the cytosine at positions 591 and 892 (C591T and C892T);adenine substituting for the guanine at position 1010 (G 1010A);adenine substituting for the guanine at position 1011 (G1011A);adenine substituting for the guanine at positions 1010 and 1011 (G1010A and G1011A);and adenine substituting for the guanine at positions 1010 and 1011 and a thyminesubstituting for the cytosine at positions 591 and 892 (G1010A; G1011A; C591T; andC892T).