Patent ID: 8673548
Filing Date: 2014-03-18
Classification: A61K,A61P,C07K,C12N,C12Q,G01N

Abstract:
1. A method of screening an agent that induces apoptosis of cells expressing TOPK comprising the steps of: (a) contacting a polypeptide selected from the group consisting of: (1) a polypeptide comprising the amino acid sequence of SEQ ID NO: 92; and (2) a polypeptide comprising the amino acid sequence of positions 32 to 318 of SEQ ID NO: 92, and that has a biological activity equivalent to the polypeptide consisting of the amino acid sequence of SEQ ID NO: 92; with a substrate phosphorylated by the polypeptide and an agent under a condition that allows phosphorylation of the substrate, wherein the substrate is histone H3 or a fragment thereof that comprises at least its phosphorylation site; (b) detecting the phosphorylation level of the substrate; (c) comparing the phosphorylation level of the substrate with the phosphorylation level of the polypeptide detected in the absence of the agent; and (d) selecting the agent that reduced the phosphorylation level of the substrate as an agent that induces apoptosis of the cells.