Patent ID: 8916345
Filing Date: 2014-12-23
Classification: C07H,C12Q,G01N

Abstract:
1. A method of detecting a target oligonucleotide in a sample, comprising: contacting the sample with a composition comprising a first oligonucleotide having the structure 5′-Y M is L R one of R wherein P is R R wherein the first oligonucleotide is adapted to hybridize to a second oligonucleotide having the structure 3′-Y wherein Y wherein Y wherein if the first oligonucleotide hybridizes to the second oligonucleotide, N wherein the first oligonucleotide is also adapted to hybridize to the target oligonucleotide; wherein the first oligonucleotide is labeled with a fluorophore; and wherein fluorescence of the fluorophore is reduced by fluorescence resonance energy transfer to the quencher or by ground state quenching by the quencher when the first oligonucleotide is not hybridized to the target oligonucleotide; and detecting an increase in fluorescence indicating the presence of the target oligonucleotide in the sample.