Patent ID: 7455983
Filing Date: 2008-11-25
Classification: C12N

Abstract:
1. A method of evaluating a media for culturing human embryonic stem (hES) cells in vitro to determine if they proliferate without differentiating, comprising culturing hES cells on an extracellular matrix and in a medium comprising: an isotonic buffer; a protein nutrient, comprising serum, serum replacement, albumin, or essential and non-essential amino acids; lipids, fatty acids, or cholesterol, either as artificial additives or as the HDL or LDL extract of serum; added fibroblast growth factor at a concentration of 40 ng/mL; and added Flt-3 ligand at a concentration of 15 ng/ml; and culturing hES cells in the medium through at least four passages; and determining if the hES cells cultured in the medium have differentiated.