Patent ID: 6361947
Filing Date: 2002-03-26
Classification: C12Q,Y10S

Abstract:
A method of analyzing a first nucleic acid sample comprising:providing said first nucleic acid sample; obtaining a second nucleic acid sample by: (a) fragmenting said first nucleic acid sample to produce fragments, ligating adaptor sequences to said fragments, amplifying at least some of said fragments, and isolating said amplified fragments; or (b) fragmenting said first nucleic acid sample to produce fragments, denaturing said fragments, allowing at least some of said fragments to reanneal to form double stranded DNA sequences and removing said double stranded DNA sequences thus isolating said single stranded fragments; or (c) amplifying said first nucleic acid sample by arbitrarily primed PCR to produce an amplification product and isolating said amplification product; or (d) fragmenting said first nucleic acid sample to produce fragments, hybridizing said fragments to an oligonucleotide probe bound to a solid support, and isolating said hybridized fragments; or (e) fragmenting said first nucleic acid sample to produce fragments, binding said fragments to a mismatch binding protein, and isolating said bound fragments; providing a nucleic acid array; hybridizing said second nucleic acid sample to said array; and analyzing a hybridization pattern resulting from said hybridization.