Patent ID: 7202069
Filing Date: 2007-04-10
Classification: C12N,C12P

Abstract:
1. A method for producing an (S)-4-halo-3-hydroxybutyric acid ester derivative, the method comprising reacting an (R)-2-octanol dehydrogenase having a molecular weight of about 30,000 Da as determined by SDS-PAGE and about 83,000 Da as determined by gel filtration, or a microorganism producing the (R)-2-octanol dehydrogenase, with a 4-haloacetoacetic acid ester derivative to reduce the 4-haloacetoacetic acid ester derivative, wherein the (R)-2-octanol dehydrogenase is a polypeptide chosen from (a) or (b) below: (a) polypeptide comprising an amino acid sequence at least 95% identical to the amino acid sequence of SEQ ID NO:2; or (b) a polypeptide comprising the amino acid sequence of SEQ ID NO:2.