Patent ID: 7192750
Filing Date: 2007-03-20
Classification: C12P

Abstract:
1. A method of producing an enhanced Actinosynnema strain that produces an ansamitocin in an amount of between about 1.2-fold and about 10-fold more than the amount produced by a parental Actinosynnema strain from which the enhanced strain is derived, said method comprising: (a) treating a bacterial culture of an (b) growing the treated bacteria of (a) under selective pressure, (c) selecting an isolate from the product of (b) that exhibits increased production of an ansamitocin compared with the culture used in (a), and (d) optionally repeating (a), (b) and (c) until an isolate that produces between about 1.2-fold and about 10-fold more of an ansamitocin than the culture used in (a) is obtained.