Patent ID: 6858399
Filing Date: 2005-02-22
Classification: G01N,Y10S

Abstract:
1. A method of evaluating potential oxidative stress and/or cytotoxic response changes on cells from a particular individual to at least one agent of interest comprising the steps of: 1) obtaining a sample containing cells from said individual and, without intervening tissue culture, preparing at least two suspensions of said cells from said sample in isotonic solutions; 2) preparing a control solution containing detectably labelled choline, and adding one of said at least two non-cultured suspensions thereto to form a control suspension; 3) preparing at least one test solution each containing detectably labelled choline and said at least one agent of interest, then adding a different one of said at least two non-cultured suspensions to each of said at least one test solution to form at least one test suspension; 4) incubating said control and at least one test suspensions for 2-90 minutes under conditions suitable for incorporation of said detectably labelled choline into phosphatidylcholine in a lipid phase of the said individual's cells therein; 5) stopping said incubating by adding an organic solvent to each of the control and test suspensions; 6) extracting and drying the lipid phase of the cells in each of said stopped control and test suspensions; and 7) detecting and measuring labelled choline incorporated into each lipid phase, and comparing a level of choline incorporation into phosphatidylcholine measured in the lipid phase of cells in said at least one test suspension to a level of incorporation measured in said control suspension, to identify differences in the levels of labelled choline incorporation in order to evaluate oxidative stress and/or cytotoxic response effects induced by said at least one agent of interest on the cells of said individual.