Patent ID: 6403319
Filing Date: 2002-06-11
Classification: A61K,C07K,C12Q,Y10T

Abstract:
A method of identifying nucleic acid fragments in nucleic acid samples, the method comprising(a) mixing one or more nucleic acid samples with one or more different hairpin primers, wherein each hairpin primer comprises a different primer sequence, (b) incubating the samples under conditions that promote amplification of nucleic acids in the samples, wherein amplified nucleic acid fragments are formed which have hairpin primer sequences at one or both ends, (c) incubating the samples under conditions that promote formation of hairpin structures by the hairpin primer sequences at the ends of the amplified fragments, (d) hybridizing each sample with a plurality of detector probes and covalently coupling the hairpin structures to the probes, wherein each probe has a different sequence, and (e) detecting, directly or indirectly, coupling of the amplified fragments to the detector probes.