Patent ID: 9150930
Filing Date: 2015-10-06
Classification: C12Q

Abstract:
1. A method for detecting nucleic acid of a mycobacterium belonging to the Mycobacterium tuberculosis (MTBc) in a sample, the method comprising: (a) contacting the sample with a pair of primers comprising a forward oligonucleotide primer and a reverse oligonucleotide primer, wherein said forward primer hybridizes to a target nucleic acid sequence that is located within multiple Mycobacterial Interspersed Repetitive Unit (MIRU) repeat elements, and wherein said reverse primer hybridizes to a target nucleic acid sequence that is located within the multiple MIRU repeat elements; wherein the forward primer comprises a sequence that is at least 95% identical to a nucleotide sequence selected from SEQ ID NOs: 25-39; and wherein the reverse primer comprises a sequence that is at least 95% identical to the complement of a nucleotide sequence selected from SEQ ID NOs: 40-46; (b) extending said forward and reverse primers to generate at least one amplification product; and (c) detecting the at least one amplification product.