Patent ID: 6168927
Filing Date: 2001-01-02
Classification: C07K,C12N,C12Q,G01N

Abstract:
A method of testing a compound for the ability to affect the rate of dissociation of G.alpha. from G.beta..gamma. in a cell, comprising: providing a transformed yeast cell containing a first heterologous DNA sequence which codes for a heterologous G protein coupled receptor and a second heterologous DNA sequence which codes for a heterologous G.alpha., wherein said first and second heterologous DNA sequences are expressed, at least under the conditions of testing, in said cell, wherein said cell does not express the endogenous G.alpha., wherein said cell expresses G.beta..gamma., wherein said heterologous G.alpha. and said G.beta..gamma. can complex to form a functional G protein, and wherein said heterologous G protein coupled receptor, when activated by said compound, can operatively associate with said G protein to increase the rate of dissociation of G.alpha. from G.beta..gamma. in said G protein; contacting said compound with said cell; and detecting the rate of dissociation of G.alpha. from G.beta..gamma. in said cell.