Patent ID: 8703497
Filing Date: 2014-04-22
Classification: C12Q,G01N,Y10T

Abstract:
1. A system for analyzing nucleic acids, comprising: a nanowire assembly including a plurality of nanowires, at least two nanowires each coupled to a different probe corresponding to a different sequence version of a polymorphism; a reagent delivery system capable of contacting the nanowire assembly sequentially with a template polymorphic nucleotide, an extension nucleic acid, and an enzyme; an electrical detector configured to measure an electrical characteristic of the nanowire assembly; a controller in communication with the reagent delivery system and the electrical detector and configured to collect data corresponding to changes in the electrical characteristic in response to contact with the template polymorphic nucleotide, the extension nucleic acid, and the enzyme; and a heater configured to apply heat to the nanowire assembly to change a temperature sufficient to disrupt a nucleotide assembly at least including a probe and the template polymorphic nucleotide, wherein the controller is in communication with the heater to control heating of the nanowire assembly to facilitate disruption of the nucleotide assembly.