Patent ID: 8536318
Filing Date: 2013-09-17
Classification: C07H

Abstract:
1. A method of synthesis of a single-stranded RNA, characterized in that it comprises the following steps: a) binding, to a solid substrate, of a monomer of the following formula II in which: in which X is O or S, R′ is H or CH b) assembly, with the monomer of formula II bound to its substrate obtained in step a), of at least one monomer of the following formula III: in which X c) release of the protected single-stranded RNA bound to a substrate obtained in step b), by a method of released comprising a step a1) of treatment of the protected single-stranded RNA bound to a substrate of formula I with a base selected from piperidine, 1,8-diazabicyclo5.4.0 undec-7-ene (DBU), triethylamine at room temperature, to release the phosphate of the 3′-5′ internucleoside linkers, when X