Patent ID: 8652797
Filing Date: 2014-02-18
Classification: A61K,C12Q,G01N,Y02A

Abstract:
1. A method of NAD-dependent deacetylation of at least one lysine residue in an acetylated protein, comprising: combining the acetylated protein, with an isolated Sir2 protein or a fragment of a Sir2 protein in the presence of NAD and in the absence of other deacetylases, wherein the fragment of the Sir2 protein comprises an amino acid sequence selected from the group consisting of the amino acid sequence of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID NO: 23, SEQ ID NO: 24, SEQ ID NO: 27, SEQ ID NO: 28 and SEQ ID NO: 38; and detecting NAD-dependent deacetylation of at least one lysine residue in the acetylated protein.