Patent ID: 7572612
Filing Date: 2009-08-11
Classification: C12N,C12P

Abstract:
1. A method for the production of para-hydroxycinnamic acid comprising: (a) providing a thermostable tyrosine ammonia lyase enzyme; (b) contacting the enzyme of (a) with tyrosine wherein para-hydroxycinnamic acid is produced; and (c) optionally recovering said para-hydroxycinnamic acid; Wherein the thermostable tyrosine ammonia lyase enzyme is encoded by an isolated nucleic acid molecule selected from the group consisting of: (i) an isolated nucleic acid molecule encoding the amino acid sequence as set forth in SEQ ID NO:1; (ii) an isolated nucleic acid molecule that hybridizes with (i) under the following hybridization conditions: 0.1×SSC, 0.1% SDS, 6500 and washed with 2×550, 0.1% SOS followed by 0.1×SSC, 0.1% SDS; and (iii) an isolated nucleic acid molecule that encodes a polypeptide having 95% identity based on the Clustal method of alignment when compared to a polypeptide having the sequence as set forth in SEQ ID NO:1.