Patent ID: 6146872
Filing Date: 2000-11-14
Classification: A61K,C07K,C12N,C12P

Abstract:
An isolated acylase enzyme, wherein the acylase enzyme obtained from Oidiodendron sp. No. 30084 (FERM BP-5943), wherein the acylase catalyzes deacylation of the acyl group R.sup.1 of a cyclic lipopeptide compound of the following general formula (I): ##STR6## wherein R.sup.1 is acyl;R.sup.2 is hydroxy or acyloxy;R.sup.3 is hydrogen or hydroxy;R.sup.4 is hydrogen or hydroxy;R.sup.5 is hydrogen or hyroxysulfonyloxy; andR.sup.6 is hydrogen or carbamoylor a salt thereof, to give a cyclic peptide of the following general formula (II): ##STR7## wherein R.sup.2, R.sup.3, R.sup.4, R.sup.5, and R.sup.6 are the same groups as respectively defined above or a salt thereof: wherein the optimum pH for deacylation is from 2 to 4: the optimum temperature for deacylation is from 25 to 45.degree. C.; the enzyme activity is activated in a concentration dependent manner in the presence of methanol up to 20% by volume in the reaction mixture and the enzyme activity is inhibited at concentrations of methanol over 40% by volume.