Patent ID: 6432671
Filing Date: 2002-08-13
Classification: A61K,C07K,C12N

Abstract:
A test kit for determining the catalytic activity of a Tryparedoxin (TXN) protein, said protein having an activity which transfers reductive equivalents of trypanothione (T(SH)2) to a peroxiredoxin, said TXN protein having a molecular weight of between 15 kDA to 19 kDa as determined by mass spectrometry and having a WCPPC motif, wherein the test kit comprises:a) (T(SH)2) as a substrate of trypanothione reductase (TR), b) isolated TR as a first indicator enzyme; c) isolated tryparedoxin peroxidase (TXPNPx), as a second indicator enzyme; and d) a hydroperoxide.