Patent ID: 6783947
Filing Date: 2004-08-31
Classification: C07F,C07H,C12Q,G01N,Y02P,Y10S

Abstract:
A kit for use in a method for detecting and determining the amount of homocysteine in a sample, comprising in a packaged combination: a first reagent comprising an alkylating reagent having a haloketone or alpha haloaldehyde functional group, the carbonyl of said haloketone or alpha haloaldehyde functional group derivatized with a protected functional group said protected functional group capable of reacting with the sulfhydryl group of homocysteine to form modified homocysteine when said protected functional group is deprotected, a second reagent comprising an activating reagent capable of deprotecting said alkylating reagent by removal of the protected functional group, and a third reagent capable of specifically binding to said modified homocysteine, each in an amount sufficient to conduct at least one assay.