Patent ID: 7608583
Filing Date: 2009-10-27
Classification: C07K

Abstract:
1. A process for enhanced recovery of recombinant insulin comprising: (a) expressing the insulin in yeast, wherein the expressed insulin is secreted into the culture medium; (b) adding a water miscible organic solvent to the fermentation culture broth, wherein the fermentation culture broth contains the secreted insulin and the yeast, wherein the water miscible organic solvent is selected from the group consisting of ethanol, isopropanol, t-butanol, acetic acid, dimethylformamide, dimethylsulfoxide, acetonitrile, dioxan, ethylene glycol, and propylene glycol, and wherein the water miscible organic solvent optionally contains additives; (c) clarifying the fermentation culture broth containing the added water miscible organic solvent by centrifugation and decanting the supernatant from the pellet; (d) isolating the insulin from the supernatant.