Patent ID: 7494820
Filing Date: 2009-02-24
Classification: C07F,G01N,Y10S,Y10T

Abstract:
1. A method of determining the presence of an analyte of interest which binds to a chemical moiety, which comprises (a) forming a reagent mixture comprising the chemical moiety and a sample that may contain the analyte of interest; such that the chemical moiety and the analyte specifically bind to one another, said chemical moiety having the formula wherein M is a lanthanide; P is a polydentate ligand of M; L t is an integer equal to or greater than 1; B is a biological substance or a synthetic substance which is capable of specifically binding to the analyte of interest; P, L P, L (b) exposing the reagent mixture to electrochemical energy, the potential of which oscillates between a potential sufficiently positive and sufficiently negative to induce the chemical moiety to oscillate between at least two oxidization states, thereby inducing the chemical moiety to repeatedly electrochemiluminesce; and (c) detecting emitted luminescence thereby to determine the presence of the analyte of interest.