Patent ID: 7601498
Filing Date: 2009-10-13
Classification: C09B,C12Q,G01N,Y10S,Y10T

Abstract:
1. A method of determining presence or absence of nucleic acid in a sample, the method comprising: exposing the nucleic acid to a fluorescent nucleic acid dye having the formula: wherein BRIDGE is a substantially aliphatic linker comprising from about 8 to about 150 non-hydrogen atoms, inclusive, and wherein the linker comprises no more than one positive charge; Q Q Q wherein R when R when R X of Formula II is selected from O and S; n of Formula II is selected from 0, 1, and 2; R R R each of R R such that, if nucleic acid is present in the sample, a complex of the fluorescent nucleic acid dye and the nucleic acid is formed; and detecting fluorescence associated with the complex or a lack thereof.