Patent ID: 7807405
Filing Date: 2010-10-05
Classification: C07K,C12P

Abstract:
1. A method of generating remodeled and homogeneous functionalized glycopeptide or glycoprotein, the method comprising: (a) preparing an acceptor GlcNAc-containing peptide or protein that accepts an oligosaccharide; and (b) enzymatically adding the oligosaccharide to the acceptor GlcNAc-containing peptide or protein catalyzed by an endoglycosidase enzyme selected from the group consisting of Endo A and Endo M, wherein the oligosaccharide is a synthetic oligosaccharide oxazoline comprising a sugar chain containing monosaccharide residues and glycosidic linkages to form an intermediate glycopeptide or glycoprotein; and (c) adding a biologically active agent or a tag to the intermediate glycopeptide or glycoprotein to form the functionalized glycopeptide or glycoprotein.