Patent ID: 9205145
Filing Date: 2015-12-08
Classification: A61K,A61P,C12N

Abstract:
1. A method of producing inactivated whole Bluetongue virus (BTV) that protects animals from viremia, the method comprising the steps of: a) treating the BTV with an inactivating agent using a 1:10 ratio of inactivating agent to BTV, while the BTV remains in a complete culture; b) homogenizing the inactivating agent/BTV mixture of step a) for at least 15 minutes; c) decanting the mixture of step b) into a container and agitating the mixture for about 24 hours; d) treating the agitated mixture resultant from step c) with an inactivating agent using a 1:20 ratio of inactivating agent to BTV therein; e) homogenizing the inactivating agent/BTV mixture of step d) for at least 15 minutes; f) decanting the mixture of step e) into a container and agitating the mixture for about 48 hours; and g) neutralizing the inactivating agent, and adjusting the final pH to about 7.2, if needed; wherein the method results in inactivation of the BTV while maintaining the immunogenicity of the BTV.