Patent ID: 6177081
Filing Date: 2001-01-23
Classification: A61K,A61P,C07K,C12N,C12Q

Abstract:
An isolated and purified marmoset or human activating virus (MAV or HAV) having the following characteristics: (1) a double-stranded DNA genome; (2) an average diameter of 100 nm as determined by electron microscopy; (3) a spherical capsid with imperfect icosahedral symmetry; (4) a buoyant density of 1.12-1.20 as determined by sucrose gradient centrifugation; (5) major antigens of 32, 44, 48, 58.8, and 106.5 kDa as determined by Western blot analysis and silver staining of SDS-PAGE-resolved proteins obtained from MAV- or HAV-infected OMK cell lysates; (6) minor antigens of 26, 30.5, 74.5, 80, 95, 126.8, 168, and 198 kDa as determined by Western blot analysis and silver staining of SDS-PAGE-resolved proteins obtained from MAV- or HAV-infected OMK cell lysates; (7) a replication deficiency in HELA, VERO, CCRF-CEM, MRC-5, WI-38, SW-13, SW-47, K562, RPMI-2650, Ramos, and NIH-3T3 cell lines as it applies to MAV; (8) replication competence in OMK cell cultures as it applies to both MAV and HAV; and (9) the absence of Mg.sup.2+ -dependent reverse transcriptase activity.