Patent ID: 7728192
Filing Date: 2010-06-01
Classification: C12N

Abstract:
1. A process based on a source-sink principle for converting storage reserves in transgenic dicotyledonous seeds to a composition comprising one or more desired gene products, said process comprising steps of: a) cultivating a transgenic dicotyledonous plant having an expression system inducible during germination, said expression system comprising one or more DNA sequences encoding one or more gene products of interest, said DNA sequences being operably linked to promoter sequences and said promoter sequence having their highest expression in cotyledons of germinating seeds; b) harvesting seeds of the transgenic plants c) germinating the transgenic dicotyledonous seeds in confined conditions, during which germination the expression system is induced and the storage reserves are mobilized; d) harnessing the expression system integrated in the plant genome for de novo synthesis of one or more of desired heterologous proteins from the storage reserves mobilized in step (c); e) inhibiting protein synthesis in the chloroplast by adding antibiotics to the germination media; f) stopping the germination before de novo synthesis declines; and g) recovering the composition.