Patent ID: 7286973
Filing Date: 2007-10-23
Classification: C12N,G16B,G16C,Y02A

Abstract:
1. A method of screening for compounds that inhibit the non-mevalonate isoprenoid biosynthesis pathway, said method comprising: a) employing the three-dimensional structural coordinates of 4-diphosphocytidyl-2-C-methylerythritol (CDP-ME) synthase according to any one of Appendices 1-3 to determine the points of interaction between said CDP-ME synthase and a substrate or a substrate mimic; b) selecting compound(s) which have similar points of interaction with said CDP-ME synthase as the substrate or substrate mimics as determined in (a), wherein said compounds are selected by docking a three-dimensional representation of a candidate compound structure with a three-dimensional representation of CDP-ME synthase employed in (a); and c) assaying the activity of said CDP-ME synthase in the presence and absence of the selected compound in (b) to determine if said compound(s) is an inhibitor.