Patent ID: 6767714
Filing Date: 2004-07-27
Classification: G01N,Y10S

Abstract:
A method for determining the presence of an analyte in a liquid sample, the method comprising:a. providing a biphasic substrate comprising (1) a release medium of a first material and, in fluid communication and downstream thereof, (2) a capture medium of a microporous, second, different material, wherein located at the release medium of the biphasic substrate for release therefrom is (i) a labeled conjugate comprising a binding member reactive with a first epitope of the analyte labeled with a detectable marker, and (ii) a capturable component reactive with a second epitope of the analyte, such that if the analyte is present in the sample, the analyte produces a complex comprising the labeled conjugate, the analyte and the capturable component, and located at the capture medium of the biphasic substrate is a capture site for capturing the complex, the capture site having immobilized thereon a capture component having a binding affinity for the capturable component; b. contacting the biphasic substrate with the liquid sample such that the liquid sample travels downstream from the release medium to the capture site; and c. determining whether the analyte is present in the sample by observing the presence of the detectable marker at the capture site.