Patent ID: 7358089
Filing Date: 2008-04-15
Classification: C12N

Abstract:
1. Process for producing a substrate for attachment of cells, comprising the following process steps: providing a support structure comprising a hydrophobic resin base material, a hydrophilic polymer layer formed on the hydrophobic resin base material, and a chemical reaction product on a boundary surface between the hydrophilic polymer layer and the hydrophobic resin base material produced by reaction of the hydrophilic polymer material with hydrophobic resin of the hydrophobic resin base material; irradiating the support structure with UV radiation emitted from a light source via a pattern mask, UV irradiation of the support structure corresponding to the pattern of the pattern mask removing areas of the hydrophilic polymer layer and the chemical reaction product present on the boundary surface between the hydrophilic polymer layer and the hydrophobic resin base material so that the areas of the hydrophobic resin base material are exposed to produce said substrate for attachment of cells; wherein, following the providing and irradiating steps, the substrate produced is subjected to a surface treatment process step in which one of the following surface treatments (1) to (3) is carried out for removal of any material originating from at least one of the hydrophilic polymer layer and the chemical reaction product which have been removed, or any by product thereof, that has adhered to the exposed areas of the hydrophobic resin base material: (1) UV irradiation treatment in which irradiation is carried out with UV radiation which is emitted from an excimer lamp or a low pressure mercury lamp, (2) oxygen-plasma treatment, (3) inert gas-plasma treatment.