Patent ID: 7968309
Filing Date: 2011-06-28
Classification: C12N

Abstract:
1. A purified sucrose phosphorylase having improved thermostability, which is obtained by modifying a natural sucrose phosphorylase, wherein the sucrose phosphorylase having improved thermostability has an amino acid residue which is different from that of the natural sucrose phosphorylase in at least one position selected from the group consisting of: wherein enzyme activity of the sucrose phosphorylase having improved thermostability at 37° C., after heating the sucrose phosphorylase having improved thermostability in 20 mM Tris buffer (pH 7.0) at 55° C. for 20 minutes, is 20% or more of enzyme activity of the sucrose phosphorylase having improved thermostability at 37° C., before heating wherein the amino acid sequence of the natural sucrose phosphorylase has at least 95% sequence identity to the sequence of SEQ ID N: 2.