Patent ID: 6589741
Filing Date: 2003-07-08
Classification: C07K,C12N

Abstract:
A method for identifying a nucleic acid molecule encoding a peptide or polypeptide that interacts with a target molecule comprising the steps of:(a) translating a population of mRNA molecules devoid of stop codons in the correct reading frame in an in vitro prokaryotic translation system under conditions that allow the formation of polysomes, wherein said translation system is free of ssrA-RNA, and wherein said population of mRNA molecules is protected from ribonuclease activity; (b) bringing the polysomes so formed into contact with said target molecules under conditions that allow the interaction of the peptides or polypeptides encoded by said mRNA molecules and displayed by said polysomes with said target molecules; (c) separating polysomes displaying peptides or polypeptides that interact with said target molecules from polysomes displaying no such pep tides or polypeptides; and (d) identifying the nucleic acid molecule encoding a peptide or polypeptide displayed in a polysome that interacts with said target molecules.