Patent ID: 9201005
Filing Date: 2015-12-01
Classification: G01N

Abstract:
1. A flow cytometer system, comprising: a flow cell for streaming a hydro-dynamically focused liquid sample past an interrogation zone; a light source positioned to irradiate the interrogation zone with a light beam; a plurality of beam shaping optics disposed between the light source and the flow cell, wherein the beam shaping optics manipulate the light beam and create a horizontally flat beam profile that irradiates the liquid sample at the interrogation zone; an intermediate angle scatter (IAS) light detector positioned to measure intermediate angle scatter emitted from the liquid sample when the liquid sample is irradiated; and a mask disposed horizontally across a portion of the IAS light detector and positioned between the flow cell and the IAS light detector to cover at least a central portion of the IAS light detector so as to block a horizontal diffraction pattern created by the flat beam profile irradiating the liquid sample while allowing all IAS to be detected by the IAS light detector, wherein the mask comprises a central hole that is positioned to allow one or more axial light loss (ALL) measurements through the central hole.