Patent ID: 6194553
Filing Date: 2001-02-27
Classification: C07K,Y10S

Abstract:
A method of purifying alpha-1-proteinase inhibitor (.alpha..sub.1 -PI) from a solution comprising .alpha..sub.1 -PI and non-.alpha..sub.1 -PI contaminants, the method comprising:(a) conducting cation exchange chromatography under conditions sufficient to bind the non-.alpha..sub.1 -PI contaminants to the cation exchange substrate while not binding the .alpha..sub.1 -PI; and(b) conducting immobilized nickel affinity chromatography under conditions sufficient to bind .alpha..sub.1 -PI to the nickel affinity substrate, wherein the .alpha..sub.1 -PI is purified to a level of greater than 99.99 g .alpha..sub.1 -PI/100 g total protein.