Patent ID: 7241447
Filing Date: 2007-07-10
Classification: A61K,A61P,C12N

Abstract:
1. A composition comprising at least two recombinant adeno-associated viruses (AAV), comprising: a) a first recombinant AAV comprising a first recombinant DNA molecule comprising linked: b) a second recombinant AAV comprising a second recombinant DNA molecule comprising linked: wherein the recombinant DNA molecules of the two rAAVs, when contacted with a host cell, become linked, forming a molecule which has the cis-acting heterologous transcriptional regulatory element 5′ to the open reading frame, wherein the cis-acting heterologous transcriptional regulatory element is positioned in the first recombinant DNA molecule so that after linking the cis-acting heterologous transcriptional element regulates transcription of the gene product encoded by the open reading frame, wherein if the cis-acting heterologous transcriptional regulatory element is an enhancer, transcription of the open reading frame is enhanced by the enhancer, wherein if the cis-acting heterologous transcriptional regulatory element is a promoter, transcription of the open reading frame is initiated at the promoter, and wherein the first recombinant DNA molecule does not encode a protein.