Patent ID: 6139782
Filing Date: 2000-10-31
Classification: C07F,C12Q,Y10S

Abstract:
A method for detecting an analyte in a sample by a chemiluminescent assay procedure which comprises:(a) reacting a phosphatase enzyme with at least one compound of formula I to produce chemiluminescence for detecting the analyte, ##STR38## wherein in the compound of formula I, Het is a heterocyclic ring system comprising at least one five or six-membered ring which comprises one nitrogen atom and at least one atom selected from O and S atoms, wherein Z is selected from the group consisting of O and S atoms, wherein R.sub.6 is an organic group which allows chemiluminescence to be produced, wherein each M is independently selected from H and a cationic center and wherein n is a number which satisfies electroneutrality;(b) detecting the chemiluminescence; and(c) relating the amount of the chemiluminescence to the amount of the analyte.