Patent ID: 9206440
Filing Date: 2015-12-08
Classification: A61K,A61P,C07K,C12N

Abstract:
1. A method for killing a virus-infected cell expressing a viral antigen on the surface thereof in a subject comprising administering a retroviral vector that encodes a chimeric immune receptor wherein the one or more retroviral vectors comprise: a first nucleic acid sequence that encodes a first polypeptide sequence that binds an antigen of the virus expressed on the cell surface, and a second nucleic acid sequence that encodes a second polypeptide sequence that binds the antigen of the virus expressed on the cell surface, wherein: (a) the first polypeptide sequence and the second polypeptide sequence comprise an identical polypeptide sequence of greater than 15 amino acids in length; (b) the first nucleic acid sequence and the second nucleic acid sequence contain at least one non-identical codon within the codons that encode the identical polypeptide sequence of the first and second nucleic acid sequences; (c) at least 20% of the codons of the first and second nucleic acid sequences that encode the identical polypeptide sequence are non-identical so that homology between the first and the second nucleic acid sequences is insufficient to cause homologous recombination between the first and the second nucleic acid sequences, and (d) the retroviral vector comprises a mutant nucleic acid sequence selected from the group consisting of the SEQ ID NO:1, SEQ ID NO:4, SEQ ID NO:7, SEQ ID NO:16, SEQ ID NO:20, and SEQ ID NO:22, to the subject under conditions in which the chimeric immune receptor is expressed in a host cell of the subject, wherein when the host cell expressing the chimeric immune receptor binds to the antigen expressed on the surface of the virus-infected cell, an immune response to the virus is induced, thereby killing the virus-infected cell of the subject.