Patent ID: 8329420
Filing Date: 2012-12-11
Classification: G01N

Abstract:
1. A method of screening for a drug for the treatment of inflammatory disease, sepsis, severe sepsis or septic shock, comprising the following steps (a) to (d): (a) preparing a cell that expresses TLR4 (toll-like receptor 4) and contains a gene under regulation of a promoter containing an NF-κB (nuclear factor κB) or IRF3 (interferon regulatory factor 3) binding sequence (sample 1), and a cell that expresses a mutant TLR4 comprising SEQ ID NO: 2, except that the 747 (b) culturing sample 1 and sample 2 each in the presence of a test compound, (c) measuring the expression of the gene in sample 1 and sample 2 after culture, and (d) selecting, when the level of gene expression in sample 1 decreases by not less than about 20% than that in sample 2, the test compound as a substance that binds to TLR4 via the intracellular region of TLR4 to inhibit signal transduction from TLR4.