Patent ID: 8301388
Filing Date: 2012-10-30
Classification: C12N,C40B

Abstract:
1. A method for identifying an individual genomic clone DNA insert from a collection of genomic DNA clones comprising the steps of: a. arraying the individual genomic DNA clones in 96-well, 384-well, 864-well, or 1536-well microtiter plates comprised of rows and columns with each genomic DNA clone having a specific coordinate locating a well position defined by three or four pools chosen from the group consisting of a plate pool, a row pool, a column pool, and a diagonal pool in a hierarchical structure that is composed of a plate number, a row letter and a column number; b. culturing the collection of genomic DNA clones so that a complete set of intermediate subpools is constructed by combining an all individual genomic DNA clone culture in accordance with the hierarchical structure; c. isolating an all genomic DNA clone DNA from the all individual genomic DNA clones in the complete set of the intermediate subpools and storing in a stable form; d. repooling the complete set of intermediate subpooled genomic DNA clone DNA into a number of Final Screening Pools based on a chosen repooling design, wherein the all individual genomic DNA clone DNA is in at least 4 Final Screening Pools and no more than 8 Final Screening Pools; e. screening the number of Final Screening Pools for a DNA sequence of interest, using a screening method selected from the group consisting of Polymerase Chain Reaction (PCR) probing, DNA to DNA hybridization probing, and RNA to DNA probing, to determine the specific coordinate using the chosen repooling design and identify the well position of the DNA sequence of interest.