Patent ID: 6642052
Filing Date: 2003-11-04
Classification: A61K,C12N

Abstract:
A method of generating a recombinant adenovirus deleted for an essential gene of a late transcriptional region of adenoviral genome, said recombinant adenovirus having the essential endogenous gene expressibly cloned into a second transcriptional region of adenoviral genome, said method comprising the steps of:providing a first piece of DNA consisting of the adenoviral genome or a substantial part thereof, said first piece of DNA being deleted for the essential gene In the late transcriptional region. providing a second piece of DNA, said second piece of DNA comprising the second transcriptional region of adenoviral genome or a part thereof, said second region of adenoviral region or a part thereof comprising a first expression cassette for expressing the essential gene, said first and second pieces of DNA comprising a common polynucleotide sequence permitting homologous recombination thereof in vivo, transfecting non-complementing cells capable of hosting the recombinant adenovirus with the first and the second pieces of DNA. incubating said cells, and harvesting the recombinant adenovirus.