Patent ID: 8263022
Filing Date: 2012-09-11
Classification: B01L,B33Y,G01N

Abstract:
1. A method for introducing an analyte of interest in a sample to a microfluidic device, comprising: providing an aqueous sample in a large volume reservoir, said sample containing the analyte and having a volume of greater than 10 microliters, wherein the analyte of interest is charged or has been associated with a charged molecule; providing a microfluidic device comprising an analysis area; providing a connector, wherein the large volume reservoir and the microfluidic device are fluidically connected via the connector; electrophoresing the analyte from the large volume reservoir to the microfluidic device via the connector; and further transporting the analyte to the analysis area for a time sufficient to result in a higher concentration of analyte in the analysis area than the concentration in the sample, wherein the analyte is continuously flowed from the large volume reservoir to the analysis area during said time.