Patent ID: 8524465
Filing Date: 2013-09-03
Classification: G01N

Abstract:
1. A method for identifying an antagonist of hTAS2R14 bitter taste receptor activity, wherein said hTAS2R14 bitter taste receptor activity is mediated by a hTAS2R14 bitter taste receptor comprising: (a) a polypeptide comprising the amino acid sequence according to SEQ ID NO: 2; or (b) a polypeptide comprising not more than fifteen (15) amino acid residues that are conservatively substituted compared to SEQ ID NO: 2 and has at least 20% hTAS2R14 bitter taste receptor activity; or (c) a polypeptide having hTAS2R14 bitter taste receptor activity and where said polypeptide is at least 95% identical to a polypeptide having the amino acid sequence according to SEQ ID NO: 2; (1) contacting a hTAS2R14 bitter taste receptor or a host cell expressing said hTAS2R14 bitter taste receptor with a potential antagonist, and (2) determining whether the potential antagonist inhibits the hTAS2R14 bitter taste receptor activity; wherein prior, concomitantly and/or after step (1) said bitter taste receptor or said host cell is contacted with chlorhexidine.