Patent ID: 6916606
Filing Date: 2005-07-12
Classification: C07D,C07F,C07H,C12Q,G01N

Abstract:
1. A method of detecting the presence or absence of an enzyme in a sample, which comprises: (a) contacting said sample with a reagent comprising a metal-containing organic compound conjugated to a polymer, wherein said polymer is a substrate for and cleaved by said enzyme and said metal-containing organic compound is capable of being induced to repeatedly emit electrochemiluminescence upon exposure to an amount of electrochemical energy from a suitable source; (b) exposing the resulting sample to an amount of electrochemical energy from a suitable source effective to induce the reagent to repeatedly emit electrochemiluminescence; and (c) detecting emitted luminescence to determine whether the enzyme is present in or absent from the sample; wherein cleavage of the polymer by the enzyme increases the electrochemiluminescence and wherein the metal-containing organic compound conjugated to the polymer comprises ruthenium.