Patent ID: 9163270
Filing Date: 2015-10-20
Classification: C08B,C12N,C12P,C12Y

Abstract:
1. A process for the preparation of hyaluronic acid in Bacillus megaterium , comprising the following steps: (a) culturing transformed bacterial host cells of (i) at least one episomal plasmid vector comprising a sequence coding for the enzyme hyaluronan synthase and a sequence coding for the enzyme UDP-glucose dehydrogenase in tandem under the control of the strong inducible T7 promoter; or (ii) at least one episomal plasmid vector comprising a sequence coding for the enzyme hyaluronate synthase, a sequence coding for the enzyme UDP-glucose dehydrogenase, a sequence coding for the enzyme UDP-glucose pyrophosphorylase and a sequence coding for the enzyme glucose 6 phosphate isomerase, under the control of the strong inducible T7 promoter; (b) recovering hyaluronic acid from the culture medium, wherein the transformed bacterial host cells of the sequences coding for enzymes UDP-glucose dehydrogenase (tuaD), UDP-glucose pyrophosphorylase (gtaB) and glucose 6 phosphate isomerase (pgi) are obtained from wherein the fermentation time is in the range between 80 and 160 hours and the product HA has a weight average molecular weight in the range 100-500 KDa or the fermentation time is in the range between 40 and 80 hours and the product HA has a weight average molecular weight in the range 500-1000 KDa or the fermentation time is in the range between 12 and 40 hours and the product HA has a weight average molecular weight in the range 1×10