Patent ID: 8470593
Filing Date: 2013-06-25
Classification: C12N

Abstract:
1. An isolated host cell comprising a recombined amplifiable vector which is the product of a method comprising: (a) introducing one or more expression cassettes into a first multiple cloning site of a first universal transfer vector wherein said first universal transfer vector comprises the plasmid map set forth in (b) introducing one or more expression cassettes, different from the cassettes introduced into said first universal transfer vector, into a second multiple cloning site of a second universal transfer vector which second multiple cloning site comprises nucleotides 620-772 of the nucleotide sequence set forth in SEQ ID NO: 1; wherein said second multiple cloning site is in said orientation or in a reverse orientation; and (c) introducing said cassettes from steps (a) and (b) into a third multiple cloning site of an amplifiable vector; wherein said amplifiable vector comprises the plasmid map set forth in (d) introducing said amplifiable vector comprising said cassettes from steps (a) and (b) into a host cell.