Patent ID: 6280973
Filing Date: 2001-08-28
Classification: A61K,C07K,C12N

Abstract:
An isolated nucleic acid encoding a mammalian methadone-specific opioid receptor wherein said nucleic acid hybridizes to a nucleic acid probe that is the complement to the sequence identified by Seq. I.D. No. 3 at a temperature of 37.degree. C. in a solution of 5.times.SSC, 50% formamide, and 5.times.Denhardt's solution, wherein hybridization is detected after washing in a solution of 0.5.times.SSC/0.1% SDS at 55.degree. C., and wherein the receptor has no specific binding to DAMGO, DPDPE, U-69,593, bremacozine, dihydromorphine, ethylketocyclazocine or beta-endorphin, and specifically binds methadone with a dissociation constant of about 10.sup.-10 M.