Patent ID: 6159750
Filing Date: 2000-12-12
Classification: G01N,Y10S

Abstract:
A method of performing a fluorescence polarization immunoassay (FPIA) for the detection and quantification of an analyte's concentration, the method comprising the steps of:a) adding one or more reagents and a sample to be analyzed to a fluid container, wherein at least one of the reagents is a diluent and the sample is at a specified dilution after the addition of the reagents;b) removing a quantity of sample and reagent from said container;c) taking a first polarization measurement of the sample;d) adding one or more reagents to the sample in the container, with no further sample addition to the container, wherein at least one of the reagents is different from the reagents added in step a) and the addition of the reagents is such that a resulting dilution of the sample is a fraction X of the dilution of the sample after step a);e) taking a second polarization measurement; andf) calculating the analyte's concentration in the sample by calculating the difference between the first and second polarization measurements, wherein at least one of the measurements is adjusted to compensate for the difference in the dilutions of the sample following step a) and step c).