Patent ID: 6524865
Filing Date: 2003-02-25
Classification: C07F,C07K,G01N

Abstract:
A method for detecting or quantitating an analyte in a sample said method comprising:(a) contacting the sample with (i) a binding partner of said analyte or a competitive binder which competes with the analyte in a specific binding interaction, said binding partner or said competitive binder being conjugated to an enzyme; (ii) a substrate to said enzyme; and, (iii) an electrochemiluminescent detectant, wherein said substrate and the resulting product of said enzyme acting on said substrate differ in their ability to generate electrochemiluminescence at an electrode in the presence of said electrochemiluminescent detectant; (b) imposing a voltage upon a working electrode in contact with said sample and thereby causing said electrochemiluminescent detectant to emit electrochemiluminescence; (c) detecting or measuring the electrochemiluminescence emitted in step (b); and (d) comparing said detection or measurement to a standard to determine whether or in what amount said analyte is present in the sample.