Patent ID: 6734023
Filing Date: 2004-05-11
Classification: G01N,Y10S,Y10T

Abstract:
A method of quantitatively determining the stability of an unpurified test protein under native conditions, the method comprising:(a) providing an unpurified test protein; (b) contacting the test protein with an exchange buffer comprising a denaturant and deuterium, the exchange buffer having a denaturant concentration; (c) contacting the test protein with a mass spectrometry matrix medium; (d) determining a change in mass of the test protein by mass spectrometry; (e) varying the denaturant concentration of the exchange buffer; (f) repeating steps (a)-(e) a desired number of times; and (g) quantitatively determining protein stability based on the change in mass of the unpurified test protein as a function of denaturant concentration, whereby the stability of a test protein under native conditions is quantitatively determined.