Patent ID: 8298758
Filing Date: 2012-10-30
Classification: C12Q,Y02A

Abstract:
1. A method for a high sensitivity multiple microorganism detection which is a method for detecting two or more microorganisms in foods having different properties by culturing the microorganisms in a medium with glucose concentration of 0.15% or less, and/or in a medium with concentration of phosphate-buffer solution of 50 mM or more or in a medium with a buffer capacity equivalent to the concentration of phosphate-buffer solution of 50 mM or more, amplifying a plurality of target genes with a single PCR reaction tube and analyzing the same, consisting of the following steps: treating at least with a lytic enzyme and/or bacteriocin having lytic activity, a nonionic surfactant and a protein denaturant; extracting DNA of the target microorganisms to be detected, consisting of removing insoluble fractions by centrifugation, and performing alcohol precipitation; and performing multiplex PCR by mixing a primer specific to the target microorganisms to be detected, wherein, at least one of the two or more microorganisms with different properties is