Patent ID: 7759477
Filing Date: 2010-07-20
Classification: A61K,A61P,C07K,C12N,C12Q,G01N,Y10S

Abstract:
1. A pol nucleic acid product produced by the process comprising: (a) providing a sample comprising a pol gene of HIV-1, HIV-2, or SIV nucleic acid; and (b) amplifying a template comprising sequence of the pol gene of the HIV-1, HIV-2, or SIV nucleic acid from the sample using a pair of purified polynucleotide primers chosen from: wherein group (i) comprises nucleotides that hybridize to the nucleic acid, wherein the hybridizing nucleotides are chosen from 2021-2039, 2620-2643, 3339-3361, and 4186-4207 of the pol gene of HIV-1 Bru; and wherein group (ii) comprises nucleotides that hybridize to the nucleic acid, wherein the hybridizing nucleotides are chosen from 2643-2620, 3361-3339, 4207-4186, and 5011-4992 of a nucleic acid sequence complementary to the pol gene of HIV-1 Bru; wherein group (iii) comprises nucleotides that hybridize to the nucleic acid, wherein the hybridizing nucleotides are chosen from 2055-2073, 2615-2638, 3333-3356, 4181-4202, and 4987-5006 of the pol gene of HIV-1 Mal; wherein group (iv) comprises nucleotides that hybridize to the nucleic acid, wherein the hybridizing nucleotides are chosen from 2073-2055, 2638-2615, 3356-3334, 4202-4181, and 5006-4987 of a nucleic acid sequence complementary to the pol gene of HIV-1 Mal; wherein group (v) comprises nucleotides that hybridize to the nucleic acid, wherein the hybridizing nucleotides are chosen from 2024-2042, 2584-2607, 3303-3325, 4150-4171, and 4956-4975 of the pol gene of HIV-1 Eli; wherein group (vi) comprises nucleotides that hybridize to the nucleic acid, wherein the hybridizing nucleotides are chosen from 2042-2024, 2607-2584, 3325-3303, 4171-4150, and 4975-4956 of a nucleic acid sequence complementary to the pol gene of HIV-1 Eli; wherein group (vii) comprises nucleotides that hybridize to the nucleic acid, wherein the hybridizing nucleotides are chosen from 2329-2349, 2971-2994, 3690-3712, 4534-4555, and 5340-5359 of the pol gene of HIV-2 ROD; wherein group (viii) comprises nucleotides that hybridize to the nucleic acid, wherein the hybridizing nucleotides are chosen from 2349-2329, 2994-2971, 3712-3690, 4555-4534, and 5359-5340 of a nucleic acid sequence complementary to the pol gene of HIV-2 ROD; and wherein group (ix) comprises nucleotides that hybridize to the nucleic acid, wherein the hybridizing nucleotides are chosen from 2299-2318, 2887-3010, 3606-3628, 4450-4471, and 5256-5275 of the pol gene of SIV-MAC; wherein group (x) comprises nucleotides that hybridize to the nucleic acid, wherein the hybridizing nucleotides are chosen from 2318-2299, 3010-2887, 3628-3606, 4471-4450, and 5275-5256 of a nucleic acid sequence complementary to the pol gene of SIV-MAC; wherein the amplification is carried out under conditions that result in specific amplification of the pol nucleic acid product from HIV-1,HIV-2, or SIV nucleic acid.