Patent ID: 7635577
Filing Date: 2009-12-22
Classification: A61P,C12N,C12Y

Abstract:
1. A process for preparing human α-thrombin by a genetic engineering technique which comprises the following steps: (1) culturing a transfectant animal cell transfected with an expression vector in which a DNA encoding human prethrombin-2 with an upstream prothrombin preproleader sequence is incorporated to the downstream of a chicken β-actin promoter so as to produce and accumulate human prethrombin-2 at a high expression level of at least 90 μ/ml in culture supernatant and recovering the produced human prethrombin-2, wherein said DNA encoding human prethrombin-2 with an upstream preproleader sequence has the nucleotide sequence as set forth in SEQ ID NO:11; (2) treating a solution containing human prethrombin recovered in step (1) with ecarin so as to convert human prethrombin into human α-thrombin in a solution; and (3) purifying the solution obtained after the treatment in step (2) to obtain purified human α-thrombin.