Patent ID: 7732144
Filing Date: 2010-06-08
Classification: A61P,C12Q

Abstract:
1. A method for determining a chemotherapeutic regimen comprising 5-fluorouracil, oxaliplatin, or combination thereof for treating a tumor in a patient comprising: (a) isolating mRNA from a fixed tumor tissue sample; (b) subjecting the mRNA to reverse transcriptase-polymerase chain reaction (RT-PCR) amplification using a pair of oligonucleotide primers capable of amplifying a region of the Excision Repair Cross-Complementing (ERCC1) gene, and a pair of oligonucleotide primers capable of amplifying a region of the Thymidylate Synthase (TS) gene, to obtain an ERCC1 and a TS amplified sample; (c) determining the amount of TS and ERCC1 mRNA in the amplified sample; (d) comparing the amount of TS and ERCC1 mRNA from step (c) to an amount of mRNA of an internal control gene; and (e) determining a chemotherapeutic regime comprising 5-flurouracil, oxaliplatin, or combination thereof based on the amount of TS and/or ERCC1 mRNA in the amplified sample and the threshold level for TS and/or ERCC1 gene expression.