Patent ID: 9181543
Filing Date: 2015-11-10
Classification: C07K,C12N,C12Y,C40B

Abstract:
1. An isolated modified OB-fold domain, obtainable from a naturally occurring OB-fold domain and removed from the naturally occurring protein within which it is contained, wherein: (a) at least one amino acid residue located in a β-strand of the OB-fold domain binding face is mutated by substitution as compared to the naturally occurring OB-fold domain amino acid residue, or (b) at least one amino acid residue located in a β-strand of the OB-fold domain binding face and at least one amino acid residue located in a strand of the OB-fold domain loop region are mutated by substitution as compared to the naturally occurring OB-fold domain amino acid residues, (c) at least one modified amino acid residue in a strand of the OB-fold domain loop region is mutated by substitution as compared to the naturally occurring OB-fold domain amino acid residue, wherein said isolated modified OB-fold domain is selected from the group consisting of and wherein said modified OB-fold domain is soluble and has altered binding characteristics as compared to the naturally occurring OB-fold domain, and wherein said domain specifically binds to a different binding partner than the naturally occurring OB-fold domain.