Patent ID: 8143059
Filing Date: 2012-03-27
Classification: A61K,C12N

Abstract:
1. A method to identify at least one compound expressed differentially during differentiation of embryonic cells, comprising: a. comparing expression of at least one compound expressed by a first pluripotent embryonic stem cell-derived cell population to expression of at least one compound expressed by a control cell population selected from the group consisting of: an embryonic stem cell population, progeny of the pluripotent embryonic stem cell-derived cell population, a non-embryonic stem cell-derived population, and a second pluripotent embryonic stem cell-derived cell population that is different from the first pluripotent embryonic stem cell-derived cell population; and b. identifying at least one compound that is expressed at a different level in the control cell population compared with the first pluripotent embryonic stem cell-derived cell population; whereby a difference in expression level is indicative of a compound that is expressed differentially during differentiation of embryonic cells; wherein the first pluripotent embryonic stem cell-derived cell population is not totipotent and differentiates into primitive erythroid cells, definitive erythroid cells, macrophages, neutrophils, mast cells, T cells, endothelial cells, B cells, natural killer cells, megakaryocytes, eosinophils, endothelial cell lineages, endoderm cell lineages, mesoderm cell lineages, ectoderm cell lineages and progenitors and progeny thereof, and wherein said first pluripotent embryonic stem cell-derived cell population is derived by culturing an embryonic stem cell population to obtain an embryoid body cell population, followed by culturing said embryoid body cell population under conditions effective to produce said pluripotent cell population, said conditions comprising an embryonic blast cell medium.