Patent ID: 7691614
Filing Date: 2010-04-06
Classification: C12N

Abstract:
1. A method of specifically amplifying desired regions of nucleic acid from a sample containing nucleic acid, the method comprising: (a) providing a plurality of first polymerase chain reaction (PCR) primers, each first primer having a region of fixed nucleotide sequence dimensioned and configured to hybridize to a first consensus sequence selected from the group consisting of a promoter sequence, a 3′ splice sequence, a 5′ splice sequence, an Alu repeat, a tandem repeat, a poly-A site, a lariat signal, a microsatellite sequence, and a homeobox sequence; (b) providing a plurality of second PCR primers, each second PCR primer having a region of fixed nucleotide sequence dimensioned and configured to hybridize to a second consensus sequence corresponding to the first consensus sequence and selected from the group consisting of a promoter sequence, a 3′ splice sequence, a 5′ splice sequence, an Alu repeat, a tandem repeat, a poly-A site, a lariat signal, a microsatellite sequence, and a homeobox sequence; and then (c) amplifying the nucleic acid present in the sample via the PCR using the plurality of first PCR primers and the plurality of second PCR primers under conditions sufficiently stringent such that a subset of the plurality first primers binds specifically to the first consensus sequence, and a subset of the plurality of second primers binds specifically to the corresponding second consensus sequence, and the nucleic acid flanked by the first primer and the second primer is specifically amplified to yield amplicons.