Patent ID: 6793488
Filing Date: 2004-09-21
Classification: C12Q,Y02A

Abstract:
A composition suitable for use in a process involving DNA amplification comprising DNA segments of 19-36 nucleotides in length which have sequences selected from:Serotype-specific Upstream Primers (5) DV1-1U 5â€²-GAT-CAA-GCT-TACA-CCA-GGG-GAA-GCT-GTA-TCC-TGG-3â€² (SEQ ID NO 4), DV2-2U 5â€²-GAT-CAA-GCT-TAAG-GTC-AGA-TGA-AGC-TGT-AGT-CTC-3â€² (SEQ ID NO 5), DV3-1U 5â€²-GAT-CAA-GCT-TAGC-ACT-GAG-GGA-AGC-TGT-ACC-TCC-3â€² (SEQ ID NO 6), DV4-1U 5â€²-GAT-CAA-GCT-TAAG-CCA-GGA-GGA-AGC-TGT-ACT-CCT-3â€² (SEQ ID NO 7), and JE.F2-5â€²-CAAGCCCCCTCGAAGCTGT-3â€² (SEQ ID NO 13); Serotype-specific Fluorescent probes (4) DV1-P1 5â€²-CTG-TCT-DTA-CAG-CAT-CAT-TCC-AGG-CA-3â€² (SEQ ID NO 8), DV4-P1 5â€²-CTG-TCT-CTG-CAA-CAT-CAA-TCC-AGG-CA-3â€² (SEQ ID NO 9), DV2-P1 5â€²-CTG-TCT-CCT-CAG-CAT-CAT-TCC-AGG-CA-3â€² (SEQ ID NO 1), and JE.P1 5â€²-TCTGCTCTATCTCAACATCAGCTACTAGGCACAGA-3â€² (SEQ ID NO 12); and Serotype-specific Down-stream Primer (3) DV4-1L 5â€²-CAA-TCC-ATC-TTG-CGG-CGC-TCT-3â€² (SEQ ID NO 10), DV2-1L 5â€²-GAT-CGA-ATT-CCAT-TCC-ATT-TTC-TGG-CGT-TCT-3â€² (SEQ ID NO 11), and JE.R382 5â€²-CACCAGCTACATACTTCGGCG-3â€² (SEQ ID NO 14); or the complement thereof, wherein the composition contains a) at least one of said serotype-specific upstream primers and one of said serotype-specific down stream primers or b) one of said serotype-specific fluorescent probes.