Patent ID: 6444470
Filing Date: 2002-09-03
Classification: C12N

Abstract:
A method for transforming a Zea mays plant, said method comprising:(a) administering, in planta, an effective amount of a transformation-enhancing agent by injecting into said Zea mays plant or applying topically to said Zea mays plant, said transformation-enhancing agent comprising at least one component selected from the group consisting of an auxin, an auxin analog, a cytokinin, an ABA synthesis inhibitor, an antioxidant, an inhibitor of ethylene synthesis, an inhibitor of ethylene action, Medium 604, a hormone cocktail, proline mix, salts cocktail 1 and ABA; (b) transforming a cell from said Zea mays plant with a DNA construct comprising a nucleotide sequence of interest; and (c) regenerating a transformed Zea mays plant from said cell.