Patent ID: 7935483
Filing Date: 2011-05-03
Classification: C12N,C12Q

Abstract:
1. A method of amplifying or detecting a gene of an acid-fast bacterium, comprising: extracting the gene by lysing the acid-fast bacterium, wherein the gene extracting step consists of a lipolysis process in which the acid-fast bacterium is treated with lipase and a heating process in which the acid-fast bacterium is heated in a liquid containing a non-ionic detergent and a metal chelating agent, wherein the heating process is performed after the lipolysis process, wherein the lipolysis process is performed at pH in a range of 4 to 8 and at a temperature in a range of 37° C. to 60° C. for 5 to 30 minutes, and the heating is performed at a temperature in a range of 37° C. to 100° C. for 5 to 30 minutes, and wherein a concentration of the metal chelating agent is 0.1 to 2.0 mM; and at least one step selected from amplifying the gene or detecting the gene by using the extracted gene as it is.