Patent ID: 6660524
Filing Date: 2003-12-09
Classification: C12N

Abstract:
A system for modulating expression of a target nucleic acid sequence comprising:(a) a first nucleic acid sequence operably linked to and regulates the expression of a second nucleic acid sequence, said second nucleic acid sequence encoding a regulator polypeptide; and (b) a third nucleic acid sequence operably linked to and regulates the expression of a target nucleic acid sequence, whereby said regulator polypeptide binds to said third nucleic acid sequence and modulates the expression of said target nucleic acid sequence, and wherein said regulator polypeptide comprises (i) the amino acid sequence of SEQ ID NO: 2; or (ii) an amino acid sequence encoded by a nucleotide sequence that hybridizes to the complement of the nucleotide sequence from 295 to 1035 of SEQ ID NO: 1 under hybridization conditions comprising incubating at 37Â° C. in 1Ã—SSC and 50% formamide, and/or, wherein said third nucleic acid sequence (i) comprises the nucleotide sequence from 1225 to 1260 of SEQ ID NO:1; or (ii) hybridizes to the complement of the nucleotide sequence from 1225 to 1260 of SEQ ID NO:1 under hybridizing conditions comprising incubating at 37Â° C. in 1Ã—SSC and 50% formamide.