Patent ID: 7781188
Filing Date: 2010-08-24
Classification: A61K,A61P,C07K

Abstract:
1. An isolated nucleic acid comprising a polynucleotide which hybridizes to, and along the full length of, a second nucleic acid which consists of a sequence complementary to a nucleic acid sequence which encodes the amino acid sequence of SEQ ID NO: 2, or SEQ ID NO:29, under high stringency conditions comprising: (a) hybridization in 6×SSC, 50 mM Tris-HCl (pH 7.5), 1 mM EDTA, 0.02% PVP, 0.02% of a copolymer of sucrose and epichlorohydrin, 0.02% BSA, and 100 μg/ml denatured salmon sperm DNA at 65° C.; and (b) washing in a solution containing 2×SSC, 0.01% PVP, 0.01% of a copolymer of sucrose and epichichlorohydrin, and 0.01% BSA at 37° C. for 1 h, and subsequently in 0.1×SSC at 50° C. for 45 min; wherein the polynucleotide encodes a protein that displays inhibitory activity in an NIH 3T3 fibroblast spreading assay.