Patent ID: 6958239
Filing Date: 2005-10-25
Classification: C12N

Abstract:
1. A chimeric antisense oligonucleotide comprising: a 5′ terminus; a 3′ terminus and from II to 59 5′→3′-linked nucleotides which contiguously hybridize to specific RNA and which are independently selected from the group consisting of 2′-modified phosphodiester nucleotides, and 2′-modified P-alkyloxyphosphotriester nucleotides; and wherein said 11 to 59 5′→3′-linked nucleotides are divided by an RNase H-activating region capable of contiguously hybridizing to the specific RNA and of between three and ten contiguous phosphorothioate-linked deoxyribonucleotides, and wherein the 3′ terminus of said oligonucleotide is drawn from the group consisting of: an inverted deoxyribonucleotide, a contiguous stretch of one to three phosphorothioate 2′-modified ribonucleotides, a biotin group, and a P-alkyloxyphosphodiester nucleotide, and wherein the 5′ terminus of said oligonucleotide is drawn from the group consisting of: an inverted deoxyribonucleotide, a contiguous stretch of one to three phosphorothioate 2′-modified ribonucleotides, a biotin group, and a P-alkyloxyphosphodiester nucleotide.