Patent ID: 8404659
Filing Date: 2013-03-26
Classification: A61P,C12N

Abstract:
1. A method of lowering of the activity of a microRNA target in vivo in a primate, comprising administering to the primate at least two successive effective doses of an antisense oligonucleotide targeting the microRNA target, wherein (a) the antisense oligonucleotide is essentially incapable of recruiting RNAseH, (b) at least 30% of the nucleotide monomers in the antisense oligonucleotide are LNA monomers, (c) the antisense oligonucleotide has a length of 7 to 25 nucleotides, (d) the antisense oligonucleotide does not comprise a region of more than 4 consecutive DNA monomers, and (e) the antisense oligonucleotide comprises a fully phosphorothioated backbone, and wherein the at least two successive effective doses of the antisense oligonucleotide are administered to the primate with a time interval of at least two weeks between the doses, and an effective target tissue concentration of the antisense oligonucleotide is maintained between the doses.