Patent ID: 6274724
Filing Date: 2001-08-14
Classification: C07D,C07H,C08F

Abstract:
In a method for the synthesis of oligonucleotides comprising the steps of:a) reacting a 5'-protected monomer unit with an oligonucleotide unit in the presence of a coupling agent to form a reaction mixture containing a product, said product of said reaction being a 5'-protected oligonucleotide having its length increased by joining said monomer unit to said oligonucleotide unit; andb) separating the product from the unreacted starting material, unreacted 5'-protected monomer unit, side products, and reagent; the improvement comprising:said coupling agent being a polymer consisting of cyclic imidazole units having nitrogen at the 1 and 3 positions; a carbon at each of the 2, 4 and 5 positions; and radical substituents G1 and G2 carried at respective said 4 and 5 positions, where G1 and G2 are each independently selected from a group of electron-withdrawing substituents; wherein each of said imidazole units is connected to the main polymer chain through said 2 position; providing a polymer of the formula: ##STR1##where R1 is hydrogen or a substituted or unsubstituted alkyl having up to 10 carbon atoms, R2 is hydrogen or a substituted or unsubstituted alkyl having up to 4 carbon atoms, and R3 is hydrogen.