Patent ID: 6100092
Filing Date: 2000-08-08
Classification: C12N

Abstract:
A method for amplifying a target nucleic acid comprising the step of contacting a plant host cell under conditions suitable for transforming or transfecting said cell with an isolated polynucleotide specifying a plant-active Amplification Promoting Sequence (APS), said polynucleotide selected from the group consisting of:a) a polynucleotide comprising the sequence set forth in SEQ ID NO:1; andb) a polynucleotide which hybridizes to a polynucleotide having the complement of the sequence set forth in step a), said hybridization occurring in 3.times.SSC, 20 mM NaPO.sub.4 (pH 6.8) at 65.degree. C. with washing in 0.2.times.SSC at 65.degree. C.