Patent ID: 7220564
Filing Date: 2007-05-22
Classification: C12N,C12P

Abstract:
1. An isolated polypeptide having the following physical and chemical properties (1) to (4): (1) activity: stereoselectively reducing N-benzyl-3-pyrrolidinone represented by the formula (1): with NADH or NADPH as a coenzyme, to form (R)-N-benzyl-3-pyrrolidinol represented by the formula (2): (2) optimum pH for activity: 5.5 to 6.0; (3) optimum temperature for activity: 50° C. to 55° C.; (4) molecular weight: about 55,000 as determined by gel filtration analysis, about 28,000 as determined by SDS polyacrylamide gel electrophoresis analysis, and which is isolated from a microorganism belonging to the genus