Patent ID: 7709696
Filing Date: 2010-05-04
Classification: A01K,C12N

Abstract:
1. A method of generating a transgenic mouse comprising novel transposition events in its genome by inducing gene transposition in the developing spermatocyte, comprising the steps of: a) generating a first adult transgenic mouse comprising within its genome one or more copies of a Tc/1 mariner transposon; b) generating a second adult transgenic mouse comprising within its genome one or more copies of a gene encoding a transposase cognate for said transposon, wherein said transposase is operably linked to H1t regulatory sequences such that said gene encoding said transposase is expressed only in the developing spermatocyte; c) crossing said first adult transgenic mouse with said second adult transgenic mouse to provide a first generation, male progeny which comprises, both (i) one or more copies of said transposon and (ii) one or more copies of said gene encoding said transposase cognate for said transposon, wherein said transposase is operably linked to H1t regulatory sequences such that said gene encoding said transposase is expressed only in the developing spermatocyte; d) expressing said gene encoding said transposase in said first generation male progeny to mobilize said transposon within the developing spermatocyte; e) crossing said first generation male progeny with a female, to generate second generation progeny comprising at least one transposition event within their genome; and f) identifying said transgenic second generation progeny comprising mouse progeny.