Patent ID: 8680368
Filing Date: 2014-03-25
Classification: C07K,C12N

Abstract:
1. A method of expression of a target foreign gene in a plant induced by a chemical substance, which comprises: activating with copper ions a transcription factor encoded by a foreign gene different from the target foreign gene to induce activation of transcription of the target foreign gene by a region having a promoter function contained in the target foreign gene, wherein a nucleotide sequence encoding a yeast ACE1 transcription factor is contained in a gene construct which is constructed so as to comprise a nucleotide sequence of a 5′-untranslated region of a 130k/180k gene of tomato mosaic virus that is amplified by PCR using SEQ ID NO: 20 and SEQ ID NO: 21, wherein the gene construct additionally comprises a nucleotide sequence encoding a transcriptional activation region of a VP16 transcription factor of Herpes simplex virus located downstream from the nucleotide sequence encoding said transcription factor, wherein the transcriptional activation region is that of a transcription factor different from said transcription factor, and wherein said gene construct is constructed so as to additionally comprise an arbitrary promoter upstream from the nucleotide sequence encoding said transcription factor.