Patent ID: 6130047
Filing Date: 2000-10-10
Classification: C12Q

Abstract:
A method for detecting presence of a nucleic acid target sequence comprising:a) hybridizing to the target sequence a detector nucleic acid comprisingi) a first oligonucleotide hybridized to a second oligonucleotide such that the detector nucleic acid comprises an intermolecularly base-paired portion and a single-stranded target binding sequence, and;ii) a first dye linked to the first oligonucleotide and a second dye linked to the second oligonucleotide such that fluorescence of the first or the second dye is quenched;b) extending the target sequence on the first oligonucleotide, thereby displacing the second oligonucleotide from the first oligonucleotide and producing a change in a fluorescence parameter, and;c) detecting the change in the fluorescence parameter as an indication of the presence of the target sequence.