Patent ID: 8158354
Filing Date: 2012-04-17
Classification: C12N,C12Q,H01J

Abstract:
1. A method of purifying a solution comprising a plurality of nucleic acids for subsequent analysis by electrospray mass spectrometry comprising: providing amine-terminated magnetic beads, wherein each of said amine-terminated magnetic beads comprises a magnetic bead linked to at least one amine molecule; combining said amine-terminated magnetic beads with said solution and mixing to yield a suspension of said amine-terminated magnetic beads in said solution, wherein said plurality of nucleic acids bind to said at least one amine molecule on said amine-terminated magnetic beads; isolating said amine-terminated magnetic beads from said solution; washing said amine-terminated magnetic beads to remove contaminants with one or more wash buffers while retaining said bound plurality of nucleic acids; and eluting said one or more plurality of nucleic acids from said amine-terminated magnetic beads with an elution buffer wherein said elution buffer does not comprise a metal cation salt.