Patent ID: 7737263
Filing Date: 2010-06-15
Classification: C07K

Abstract:
1. A modified Cry35 protein (Cry35-M) comprising a modified protease cleavage site that decreases the processing of Domain 3 of said protein in a plant cell and/or increases protease processing of Domain 3 in an insect cell wherein said modifications occur at one or more of the following in Domain 3: (a) modification of one or more of the four leucines occurring at positions corresponding to 353-356, (b) modification of one or more lysines at amino acids corresponding to positions 372, 376, 377, 379 or 380, (c) modification of the loop amino acids corresponding to positions 370-372 which creates a protease cleavage site, and/or (d) modification of the loop amino acids corresponding to positions 357-360 which creates a protease cleavage site; wherein said corresponding amino acid positions of said Cry35-M protein are those that correspond to the stated amino acid positions when said Cry35 protein sequence is aligned with SEQ ID NO: 1.