Patent ID: 8580931
Filing Date: 2013-11-12
Classification: B01D,C07K

Abstract:
1. A method for the isolation of alpha-1-antitrypsin (AAT) from a solution containing albumin and AAT, comprising the steps of: (a) optionally removing albumin from the solution, (b) loading the solution onto a first metal chelate chromatography substrate, wherein the chelating ligand of said substrate is iminodiacetic acid, under conditions whereby AAT is bound and retained on the first metal chelate chromatography substrate and albumin, if present, is not retained on the first metal chelate chromatography substrate; (c) selectively eluting AAT from the first metal chelate chromatography substrate; (d) loading the AAT eluate obtained from step (c) onto a second metal chelate chromatography substrate, wherein the chelating ligand of said substrate is nitrilotriacetic acid (NTA), under conditions whereby AAT remains in a flow through solution and is not bound and retained on the second metal chelate chromatography substrate; (e) collecting the flow through solution containing AAT; and (f) optionally carrying out one or more further chromatographic purification steps on the AAT solution; thereby providing an isolated AAT.