Patent ID: 6048684
Filing Date: 2000-04-11
Classification: G01N,Y10S

Abstract:
A method for the determination of soluble fibrin in a body fluid, comprising the steps of:a) binding fibrinogen to a solid phase support;b) incubating a sample of said body fluid with the solid phase bound fibrinogen whereby the soluble fibrin specifically binds to the solid phase bound fibrinogen, immobilizing the soluble fibrin;c) separating said immobilized soluble fibrin from said body fluid;d) providing an antibody able to react immunochemically with the immobilized soluble fibrin, said antibody being covalently linked to a detectable label to form a labeled antibody capable of immunochemically reacting with the immobilized soluble fibrin;e) contacting the labeled antibody with the solid phase bound fibrinogen which has been pre-incubated with said body fluid to specifically bind the label to said immobilized soluble fibrin;f) detecting said bound label; andg) determining the concentration of soluble fibrin in the body fluid from said bound labelwherein said antibody covalently linked to said detectable label is derived from an animal of the same species from which the fibrinogen is obtained but which is not the same species as the animal from which the body fluid is taken.