Patent ID: 9023273
Filing Date: 2015-05-05
Classification: A61K,A61L,A61P

Abstract:
1. A method for reducing the bioburden in a soft tissue sample, comprising: providing a soft tissue sample comprising a mammalian skin containing intact dermal cells and having at least a portion of non-dermal components removed in a container comprising an aqueous solution; and applying a pressure to the aqueous solution and time sufficient to cause at least a 5 log reduction in a bacterial load of the skin sample and to destroy substantially all of the cells within the skin sample, wherein destroying substantially all of the cells includes disrupting the cell membrane of the cells such that washing the soft tissue sample in a saline solution allows removal of at least 95% of the native skin cells, and wherein applying the pressure includes increasing the pressure at a rate to control a temperature of the skin sample such that the temperature of the skin sample does not exceed 30° C.