Patent ID: 9206439
Filing Date: 2015-12-08
Classification: C12N

Abstract:
1. A recombinant vector comprising a DNA segment having a synthetic origin of DNA synthesis that binds EBNA-1, which synthetic origin of DNA synthesis when present in the vector in a cell that expresses EBNA-1, is capable of enhanced initiation efficiency of DNA synthesis of sequences linked to the synthetic origin of DNA synthesis and maintenance of the linked sequences relative to a corresponding wild-type origin of DNA synthesis with two pairs of binding sites for EBNA-1, the pairs flanked and separated by a half-binding binding site for TRF2, wherein the synthetic origin of DNA synthesis comprises at least two pairs of binding sites for EBNA-1 that are flanked and separated by at least a half-binding site for TRF2, wherein the synthetic origin has more half-binding sites for TRF2 than the corresponding wild-type origin of DNA synthesis or has FR binding sites, Rep* binding sites or Site III binding sites, or a combination thereof.