Patent ID: 9081998
Filing Date: 2015-07-14
Classification: G01N,G06K

Abstract:
1. A method for utilizing soft X-ray microimaging for cancer cell image recognition, comprising the steps of: sample preparation; pathological examination; soft X-ray imaging; and analysis and recognition, wherein the sample preparation comprises the following steps: wherein the pathological examination comprises the following steps: wherein the soft X-ray imaging comprises the following steps: the electron microscope copper grid ultra-thin sliced samples are sent to a synchrotron radiation experimental station, in which the samples are subject to scan diffraction by synchrotron radiation soft X-rays microscopic beamlines and imaged on the photoresist, then, observing the samples with microscopic transmission and shooting a soft X-ray microscopic image, wherein a wavelength of the soft X-rays microscopic beamlines ranges from 1.0 to 10.0 nm; a water window of soft X-ray microscopy for exposure of biological samples is made of silicon nitride with different thickness options, of which the thinnest is 20 nm, wherein the analysis and recognition comprises the following steps: wherein the synchrotron radiation soft X-ray microscopic image signal is an analog signal, it must be conditioned by amplification, buffering or calibration before converted to a digital signal, so as to be suitable for being input to a subsequent signal collection unit, wherein the image signal collection comprises image pre-processing, image segmentation analysis, overlapped cell reconstruction, cell feature extraction, cell feature classification and diagnosis result output, and wherein the image pre-processing comprises grey level transformation, histogram adjustment, cell pre-processing, nucleus pre-processing and lymphocyte removal;