Patent ID: 6168934
Filing Date: 2001-01-02
Classification: C12N,C12P

Abstract:
A method of glycosylation comprising the steps of: (a) admixing in the presence of each other in an aqueous medium (i) an acceptor saccharide; (ii) a donor monosaccharide; (iii) an activating nucleotide; (iv) an activated donor monosaccharide regenerating system: (v) a pyrophosphate scavenger; and (vi) catalytic amounts of a glycosyltransferase having specificity for both the activated form of the donor monosaccharide and the acceptor saccharide and a nucleotide-sugar-pyrophosphorylase having specificity for both the donor monosaccharide and the activating nucleotide to form a reaction mixture; and (b) maintaining said reaction mixture for a time period and under conditions sufficient to glycosylate said acceptor saccharide and form a glycosylated acceptor saccharide.