Patent ID: 6635422
Filing Date: 2003-10-21
Classification: C07H,C12Q,G01N

Abstract:
A method of generating a gene expression profile of mRNA in a cell in vivo, comprising the steps of:partitioning from a cell at least one mRNA-protein (mRNP) complex comprising at least one mRNA bound to or associated with a protein, the step of partitioning comprising: contacting a biological sample comprising a mRNP complex from the cell with at least one ligand that specifically binds at least one component of the mRNP complex; separating the mRNP complex by binding the ligand with an antibody specific for the ligand, wherein the antibody is attached to a solid support; and collecting the mRNP complex by removing the mRNP complex from the solid support; and identifying the mRNA in the mRNP complex, thereby to produce a gene expression profile comprising the identity of the mRNA in the mRNP complex.