Patent ID: 7972800
Filing Date: 2011-07-05
Classification: G01N

Abstract:
1. A method of screening a compound or a salt thereof that changes the binding property or signal transduction of a G protein-coupled receptor protein comprising the amino acid sequence represented by SEQ ID NO: 1, SEQ ID NO: 6 or SEQ ID NO: 8, or a salt thereof, to an ionizable metal element or a salt thereof, comprising: (A) (i) bringing the protein in contact with the ionizable metal element, (B) (i) bringing the protein in contact with an ionizable metal element, wherein the method of screening is a method of screening for an agonist or an antagonist to said G protein-coupled receptor protein or salt thereof, wherein the ionizable metal element is selected from the group consisting of cobalt, nickel, copper, zinc or cadmium, wherein said cell-stimulating activity is selected from the group consisting of the activities that promote or suppress arachidonic acid release, acetylcholine release, intracellular Ca wherein the test compound or its salt that increases the cell-stimulating activity is an agonist of the G-protein coupled receptor protein, and the test compound or its salt that decreases the cell-stimulating activity is an antagonist for the G-protein coupled receptor protein, or (C) assaying an intracellular Ca comparing the intracellular Ca wherein the method of screening is a method of screening an agonist for said G protein- coupled receptor protein or salt thereof, wherein the ionizable metal element is selected from the group consisting of cobalt, nickel, copper, zinc or cadmium, wherein the test compound that has the intracellular Ca