Patent ID: 6967076
Filing Date: 2005-11-22
Classification: C12N,C12Q,G01N

Abstract:
1. A method for producing recombinant cells for detecting a presence of HIV in a sample, comprising: establishing a culture of cells having a reporter sequence comprising a reporter gene whose expression is regulated by a protein specific to HIV; and transducing the cells with a recombinant adenoviral vector having sequences comprising a CD4 gene and one or more cell surface coreceptor genes for HIV and an interleukin gene whose expression of an interleukin renders the transduced cell more susceptible to HIV; infection; wherein the transduced cells are more susceptible to HIV infection and express the one or more coreceptors for HIV at an elevated level such that productive infection of the transduced cells by HIV is achieved, said productive infection defined as HIV viral replication and infection of non-infected cells in the culture of the transduced cells.