Patent ID: 6703196
Filing Date: 2004-03-09
Classification: G01N,Y10S

Abstract:
A kit for determining the presence of an analyte, comprising:(a) an analytical element, (b) a binding partner 2 of a specific binding pair 1 (partner 2 of pair 1), and (c) a binding partner 2 of a specific binding pair 2 (partner 2 of pair 2), wherein the element comprises a material enabling liquid transport between a sample application zone where a sample is applied and a detection zone located downstream thereof, wherein the detection zone comprises a binding partner 1 of specific binding pair 1 (partner 1 of pair 1) immobilized in such a manner that it is able to bind to the partner 2 of pair 1 when the partner 2 of pair 1 contacts the partner 1 of pair 1, wherein the partner 2 of pair 1 is not the analyte, and wherein a labeled partner 1 of specific binding pair 2 (partner 1 of pair 2) is present upstream of the detection zone and impregnated on the material such that it can be detached by liquid and is able to bind to the partner 2 of pair 2 when the partner 2 of pair 2 contacts the partner 1 of pair 2, and the partner 2 of pair 2 is not the analyte, wherein both the partner 2 of pair 1 and the partner 2 of pair 2 (i) are not impregnated or immobilized on the material, and (ii) bind to the analyte, if the analyte is present in the sample, before the sample is applied to the application zone.