Patent ID: 6998234
Filing Date: 2006-02-14
Classification: C12Q,G01N

Abstract:
1. A method for determining a gene expression profile of vascular endothelial cells from a mixed population after the vascular endothelial cells are exposed to an anti-angiogenic agent, the method comprising the steps of: (a) contacting a mixed population of cells with a vital stain or fluorescent dye; (b) contacting said mixed population of cells with a detectable immunological reagent that specifically binds to vascular endothelial cells; (c) selecting the cells in said mixed population that bind the immunological reagent and that are not stained with the vital stain or fluorescent dye; (d) exposing the selected cells of step (c) to an anti-angiogenic agent; (e) isolating cellular RNA from the selected cells selected in step (c); (f) preparing detectably labeled cDNA or cRNA from the cellular RNA isolated in step (e); (g) hybridizing the cDNA or cRNA prepared in step (f) to a gene array comprising a plurality of eukaryotic genes; and (h) determining a gene expression profile from the hybridization pattern produced using the cDNA or cRNA preparations in step (g).