Patent ID: 7847245
Filing Date: 2010-12-07
Classification: G01N,H01J

Abstract:
1. A method for the rapid and highly sensitive analysis of cellular metabolites directly from extracts of biologic material, said method comprising: inactivating endogenous enzyme activities of the biologic material using at least one neutral organic solvent to prevent degradation of the biologic material; extracting at least one metabolite from the biologic material; developing a set of multiplexed conditions for MALDI ionization and desorption of the metabolite extracted from the biologic material on a matrix having minimal background noise that enable class selective ionization and desorption of the metabolite directly from extracts of the biological material, wherein the multiplexed conditions include varying the pH from a pH of about 3.0 to about 10.0, and wherein the selective ionization is engineered by combinatorial sets of matrix-analyte interactions.