Patent ID: 6083715
Filing Date: 2000-07-04
Classification: C07K,C12N,C12Y

Abstract:
A process for producing a heterologous polypeptide in bacteria comprising:(a) culturing bacterial cells, wherein said bacterial cells comprise an isolated nucleic acid encoding a DsbG protein, nucleic acid encoding the heterologous polypeptide, a signal sequence for secretion of both the DsbG protein and the heterologous polypeptide, and separate inducible promoters for both the nucleic acid encoding the DsbG protein and the nucleic acid encoding the heterologous polypeptide, under conditions whereby expression of the nucleic acid encoding the DsbG protein is induced prior to induction of the expression of the nucleic acid encoding the heterologous polypeptide, and under conditions whereby either both the heterologous polypeptide and the DsbG protein are secreted into the periplasm of the bacteria or the heterologous polypeptide is secreted into the medium in which the bacterial cells are cultured; and(b) recovering the heterologous polypeptide from the periplasm or the culture medium.