Patent ID: 6214556
Filing Date: 2001-04-10
Classification: C12Q

Abstract:
Method for the characterization, classification and differentiation of tissues and cell types, for the prediction of the behavior of tissues and groups of cells, and for the identification of genes with modified expression, characterized in thatin genomic DNA, which has been obtained from any tissue sample, and which may have been treated, subjected to shearing, or cleaved by means of a restriction endonuclease, the base cytosine, but not 5-methylcytosine, is converted by treatment with a bisulfite solution into uracil,fractions of the so-treated genomic DNA are amplified by the use of either very short or degenerated oligonucleotides or, in the case of cleaved or sheared genomic DNA, oligonucleotides which are complementary to adaptor oligonucleotides that have been ligated to the end of the cleaved or sheared genomic DNA before the bisulfite treatment, overall, the quantity of the remaining cytosine on a strand from the amplified fractions that contains more guanine than cytosine and/or the quantity of guanines on a strand from the amplified fractions that contains more cytosine than guanine are detected by a hybridization or polymerase reaction, which is such that the data generated in such an analysis and automatically applied to a processing algorithm make it possible to draw conclusions regarding the phenotype of the analyzed cell material.