Patent ID: 8673578
Filing Date: 2014-03-18
Classification: A23L,C07D,G01N

Abstract:
1. A method for identifying an antagonist of hTAS2R46 bitter taste receptor activity, wherein the bitter taste receptor is encoded by a polynucleotide selected from the group consisting of: (a) polynucleotide encoding at least the mature form of the polypeptide having the amino acid sequence as shown in SEQ ID NO: 10; (b) polynucleotide having the coding nucleotide sequence as shown in SEQ ID NO: 9 encoding at least the mature form of the polypeptide; (c) polynucleotide encoding a fragment of a polypeptide encoded by a polynucleotide of any one of (a) to (b), wherein said fragment has at least 95% sequence identity to SEQ ID NO: 10 and has hTAS2R46 bitter taste receptor activity; (d) polynucleotide which is an ortholog of the polynucleotide sequences shown in SEQ ID NO: 9 encoding at least the mature form of the corresponding bitter taste receptor; and (e) polynucleotide which encodes a polypeptide having hTAS2R46 bitter taste receptor activity and where said polypeptide is at least 95% identical to a polypeptide as shown in SEQ ID NO: 10; comprising the steps: (1) contacting a bitter taste receptor encoded by said polynucleotide or a host cell genetically engineered with said polynucleotide or with a vector containing said polynucleotide to express said bitter taste receptor with a potential antagonist or with a pharmaceutically acceptable salt thereof, said potential antagonist having a structure according to formula (IV): (2) determining whether the potential antagonist inhibits the bitter taste receptor activity; wherein prior to, concomitantly with and/or after step (1) said bitter taste receptor or said host cell is contacted with an agonist of bitter bitter taste receptor hTAS2R46.