Patent ID: 6060251
Filing Date: 2000-05-09
Classification: C12Q

Abstract:
A method for determining the chromosomal identity of genomic DNA containing interspersed repetitive DNA sequences, comprising the steps of: a) amplifying and labeling the genomic DNA, the amplifying being accomplished by polymerase chain reaction using primers which are substantially complementary to an interspersed repetitive DNA sequence in the genomic DNA, to produce labeled amplified fragments of the genomic DNA; b) combiningi) the labeled amplified genomic DNA fragments;ii) competitor DNA containing sequences which are substantially complementary to the interspersed repetitive DNA sequences; andiii) chromosomal DNA of known identity, under conditions appropriate for intermolecular hybridization, wherein hybridization between repetitive sequences in the labeled amplified genomic DNA fragments and in the chromosomal DNA of known identity is suppressed, thereby allowing hybridization between chromosome-specific sequences in the labeled amplified genomic DNA fragments and the chromosomal DNA of known identity;c) detecting specific hybridization of labeled amplified genomic DNA fragments to the chromosomal DNA of known identity, wherein the specific hybridization of the labeled amplified genomic DNA fragments to the chromosomal DNA of known identity indicates that the genomic DNA has the same chromosomal identity as the chromosomal DNA of known identity.