Patent ID: 6984496
Filing Date: 2006-01-10
Classification: A61P,C12Q,G01N

Abstract:
1. A method for detecting gabapentinoid activity in a test compound comprising the steps of: (a) introducing into host cells that express an NK receptor a heterologous DNA sequence that encodes a reporter polypeptide which responds to Erk-2 activation; (b) separating the host cells into at least two groups, a first group and a second group; (c) treating the first group of host cells with a test compound that binds to the α (d) treating the first group and second group of host cells with an NK receptor agonist; (e) determining reporter polypeptide activity in the first group and in the second group; and (f) comparing reporter polypeptide activity from the first group to the second group; and (g) identifying as a gabapentinoid, a test compound that shows greater inhibition of said reporter polypeptide activity in said first group of step f) than said reporter polypeptide activity of said second group in step f).