Patent ID: 6118044
Filing Date: 2000-09-12
Classification: A01K,C07K,C12N

Abstract:
A transgenic mouse having a genome which has been altered to constitutively express an IgE molecule having an immunoglobulin structure comprising:an IgE heavy chain variable region and constant region encoded by nucleotides 1 to 1683 of SEQ ID NO: 1 and an IgE light chain variable region and constant region encoded by nucleotides 1 to 714 of SEQ ID NO: 3; and wherein said IgE heavy chain constant region lacks a transmembrane region and said molecule binds IgE receptors on murine mast cells; said molecule having an antigen recognition site having specificity for an antigen having one or more trinitrophenyl groups, resulting in a phenotype selected from the group consisting of epidermal swelling following topical application of said antigen having one or more trinitrophenyl groups, systemic anaphylaxis shock with dyspnea, decrease in body temperature, and loss of motion.