Patent ID: 9206484
Filing Date: 2015-12-08
Classification: C12Q

Abstract:
1. A method for specifically detecting a Herpes Simplex Virus-1 (HSV-1) target nucleic acid in a sample comprising the steps of: (a) providing a sample suspected of containing at least a HSV-1 target nucleic acid; (b) contacting said sample with at least two amplification oligomers, wherein a first of said amplification oligomers comprises a target hybridizing sequence 15 to 25 nucleotides in length and configured to target a sequence in a region of the HSV-1 US8.5 ORF corresponding to nucleotides 124 to 156 of SEQ ID NO:1, and wherein a second of said amplification oligomers comprises a target hybridizing sequence comprising SEQ ID NO:10; and (c) performing a nucleic acid detection reaction with a detection probe configured to detect a sequence in a region corresponding to nucleotides 173 to 196 of SEQ ID NO:1 that detects an amplification product to determine whether a HSV-1 target nucleic acid is present in said sample.