Patent ID: 8145435
Filing Date: 2012-03-27
Classification: C12Q,G06K,G16B

Abstract:
1. A system for analyzing a polynucleotide-containing sample by time-dependent monitoring of an in vitro nucleic acid amplification reaction, said system comprising: an apparatus comprising a temperature-controlled incubator and a fluorescence monitoring device, said apparatus generating a data set comprising values proportional to the amount of analyte amplicon present in the in vitro nucleic acid amplification reaction as a function of time, said data set defining a growth curve; a processing device that analyzes the data set defining the growth curve, said processing device comprising software instructions to perform a vector analysis of at least a portion of said growth curve to identify a time-dependent feature indicating a transition from the baseline phase into the growth phase of said growth curve, said vector analysis comprising the steps of, an output device in communication with the processing device.