Patent ID: 8685679
Filing Date: 2014-04-01
Classification: C12N,C12P

Abstract:
1. A method for increasing the percent of polyunsaturated fatty acids in the total lipid or oil of an oleaginous Yarrowia sp., comprising: a) providing an oleaginous (i) at least one native gene encoding a phospholipid:diacylglycerol acyltransferase; (ii) at least one native gene encoding an acyltransferase selected from the group consisting of: a diacylglycerol acyltransferase 1, a diacylglycerol acyltransferase 2 and a combination of these; and (iii) at least one gene encoding a protein of a functional polyunsaturated fatty acid biosynthetic pathway; b) reducing the activity of the native acyltransferase of step a) (i) and the activity of at least one of the native acyltransferases in step a) (ii), wherein the overall rate of oil biosynthesis is reduced and the percent of polyunsaturated fatty acids in the total lipid or oil is increased in the