Patent ID: 6865492
Filing Date: 2005-03-08
Classification: C07K,G16B

Abstract:
1. A method for synthesizing a peptide or a peptide-like molecule based on matching a physicochemical mode of a peptide to the same physicochemical mode of a target polypeptide or protein, followed by synthesizing a retro-inverso peptide version of said peptide comprised of D-amino acids, comprising the steps of: assigning to each member of a set of peptide constituents a numerical value representative of the hydrophobic free eneray of said each member of a set of peptide constituents, said set of peptide constituents including all the members of the set of naturally-occurring L-amino acids; arranging said peptide constituents in order of said numerical values; partitioning said set of peptide constituents into a plurality of peptide constituent groups, whereby each of said peptide constituent groups contains at least one member of said set of peptide constituents, each peptide constituent group encompasses a range of said ordered numerical values, and each member of said set of peptide constituents belongs to only one peptide constitutent group; creating a polypeptide physicochemical data series by replacing each amino acid in an amino acid sequence of said target polypeptide or protein with said numerical value corresponding to said each amino acid in said amino acid sequence of said target polypeptide or protein; calculating one or more polypeptide eigenvalues and a corresponding polypeptide elgenvector associated with each of said one or more polypeptide eigenvalues by linear decomposition of an autocovariance matrix formed from a sequentially lagged data matrix of said polypeptide physicochemical data series; ordering said one or more polypeptide eigenvalues and said corresponding polypeptide eigenvectors from largest to smallest; selecting one or more of said polypeptide eigenvectors; transforming said one or more of said polypeptide eigenvectors into an eigenvector template; forming a graph of said eigenvector template, wherein said numerical values are graphed along the y-axis of said graph and ordered position in said cigenvector template is graphed along the x-axis of said graph; partitioning said graph along said y-axis according to said ranges of said numerical values defining said peptide constituent groups, to form a plurality of y-axis ranges; assigning one of said peptide constituents to each position in said peptide or peptide-like molecule by using said graph as a template to create a sequence of a mode-matched peptide, wherein at each ordered position in said eigenvector template along said x-axis of said graph, said one of said peptide constituents assigned to said ordered position has a numerical value that is within said y-axis range of said ordered point; determining a sequence of a retro-inverso peptide by inverting said sequence of a mode-matched peptide; and synthesizing said retro-inverso peptide from said sequence, using D-amino acids.