Patent ID: 6475748
Filing Date: 2002-11-05
Classification: G01N,Y10S

Abstract:
A method for detecting infectious or inflammatory disease in a human patient who is suspected of having inflammation or infection resulting from kidney transplant which method comprises:(a) quantifying IgG-FcRI receptors on human peripheral blood granulocytes derived from the patient by: (1) cultivating the granulocytes in the presence of human interferon-y; (2) reacting the cultivated granulocytes from step (a)(1) with an immune complex comprising mouse IgG and a chemiluminescent substance to generate chemiluminescence; and (3) measuring intensity of the chemiluminescence generated; (b) quantifying IgG-FcRI receptors on human peripheral blood granulocytes derived from the patient by: (1) cultivating the granulocytes in the absence of human interferon-y; (2) reacting the cultivated granulocytes from step (b)(1) with an immune complex comprising mouse IgG and a chemiluminescent substance to generate chemiluminescence; and (3) measuring intensity of the chemiluminescence generated; (c) calculating a CL ratio, which is the ratio of the intensity of the chemiluminescence generated in step (b) to the intensity of the chemiluminescence generated in step (a); and (d) comparing the CL ratio obtained in step (c) to a CL ratio of a healthy human subject, so as to detect the presence of infectious or inflammatory disease in the patient.