Patent ID: 8357779
Filing Date: 2013-01-22
Classification: A61K,A61P,C07K

Abstract:
1. A method for forming a proteinaceous molecule comprising a von Willebrand factor (VWF) molecule and a PEG moiety covalently and stably bound to at least one primary amino group on said VWF molecule, said proteinaceous molecule having the capability of binding at least one Factor VIII (FVIII) molecule, comprising: a) providing a VWF molecule selected from the group consisting of plasmatic VWF, recombinant VWF, and dimers and multimers thereof; b) contacting said primary amino group on said VWF molecule with a PEG reagent; and c) allowing said PEG reagent to covalently and stably bind to said primary amino group on said VWF, thereby forming said proteinaceous molecule, wherein the in vivo half-life of said proteinaceous molecule is increased as compared to the in vivo half-life of a VWF molecule and the in vivo half-life of said FVIII molecule bound to said proteinaceous molecule is increased as compared to the in vivo half-life of a FVIII molecule not bound to said proteinaceous molecule.