Patent ID: 6566077
Filing Date: 2003-05-20
Classification: C07K,G01N

Abstract:
A method for qualitative or quantitative determination of an immunoglobulin or a mixture of immunoglobulins according to the ELISA technique comprising:(i) immobilizing a compound capable of binding itself non convalently to at least one immunoglobulin on a microtiter plate for ELISA determination, (ii) incubating a sample containing the immunoglobulin or the immunoglobulins to be determined on said microtiter plate, (iii) washing said microtiter plate, (iv) testing the thus formed immobilized complex compound/immunoglobulin, wherein said compound capable of binding itself non covalently to at least one immunoglobulin is a compound of formula (I) (H2Nâ€”X1â€”Thrâ€”X2â€”CO)nâ€”Râ€ƒâ€ƒ(I) â€ƒwhere X1 and X2, different one another, are an amino acid residue of arginine or tyrosine in configuration L or D, wherein the hydroxy group of threonine and tyrosine and the guanidine moiety of arginine is optionally protected by a compound conventionally used in peptide chemistry for protecting the hydroxy group and the guanidine moiety, respectively, n is 1,2,3 or 4, and R, when n is 2,3 or 4, is a group suitable for forming a dimer, trimer or tetramer, and when n is 1, R is a single amino acid residue, or a peptide chain comprising 2 to 7 amino acid residues.