Patent ID: 9109264
Filing Date: 2015-08-18
Classification: C12Q

Abstract:
1. A composition of oligonucleotides for amplifying and detecting an HBV nucleic acid wherein said composition comprises at least two amplification oligomers configured to generate an HBV amplicon from an HBV nucleic acid present in a test sample at a concentration as low as 200 copies/ml of reaction mixture, wherein: (a) the first amplification oligomer consists of a target hybridizing sequence, optionally, an upstream sequence that is not complementary to an HBV target sequence, and a 5′ promoter sequence for an RNA polymerase, wherein the target hybridizing sequence consists of SEQ ID NO:25 or SEQ ID NO:28; and (b) the second amplification oligomer consists of a target hybridizing sequence consisting of SEQ ID NO:15.