Patent ID: 9061901
Filing Date: 2015-06-23
Classification: B01L,B82Y,C12Q,G01N,Y10T

Abstract:
1. A device for analyzing a polynucleotide macromolecule, comprising: two or more fluid reservoirs joined by a fluid pathway; a nanochannel having an effective inner diameter between about 10 nm and about 500 nm; a discrete constriction in or at a terminal end of the nanochannel, having an effective inner diameter that is no more than about 40% of the effective inner diameter of the nanochannel, wherein the nanochannel and the constriction are located in the fluid pathway between the reservoirs, wherein the constriction is capable of maintaining a linearized polynucleotide macromolecule passing across the constriction in a linearized form, wherein the constriction is configured to locally reduce the effective inner diameter of the nanochannel to be about 0.5 nm to about 100 nm; and a sensor associated with the device located to detect a signal from at least a portion of the polynucleotide macromolecule as it passes through the constriction.