Patent ID: 6455755
Filing Date: 2002-09-24
Classification: A01K,C07K,C12N

Abstract:
A method for screening lipoxin compounds that inhibit the recruitment of polymorphonuclear neutrophils (PMN) in a transgenic mouse that produces in its leukocytes recombinant human leukotriene B4 receptor (hBLTR), comprising:providing the transgenic mouse that produces in its leukocytes a recombinant hBLTR, wherein said transgenic mouse has stably integrated into its genome an exogenous expression vector that comprises a neucleotide sequence comprising a CDIIb promoter in operable linkage with a nucleotide sequence encoding said hBLTR, and wherein expression of said hBLTR results in an increased recruitment of PMN in response to a physiological stress, in said mouse; allowing said hBLTR to be produced and secreted in said leukocytes of said transgenic mouse; physiologically stressing said transgenic mouse, thereby causing increased neutrophil recruitment to the area of physiological stress; administering a lipoxin compound to interact with said neutrophil recruitment in response to said physiological stress; and determining whether said lipoxin compound reduces neutrophil recruitment in response to said physiological stress.