Patent ID: 8809011
Filing Date: 2014-08-19
Classification: A61K,A61P,C12Q,C12Y,G01N

Abstract:
1. A method of identifying a compound that decreases proline hydroxylation of the alpha subunit of hypoxia inducible factor (HIFα), the method comprising: a) contacting a compound and a non-hydroxylated HIFα polypeptide comprising amino acids 556-575 of human HIF1α, numbered in accordance with wild type HIF1α, under conditions wherein the HIFα, polypeptide, in the absence of compound, is hydroxylated on at least one proline residue; b) measuring the degree of prolyl hydroxylation of the HIFα polypeptide in the presence of compound; and c) comparing the degree of prolyl hydroxylation of the HIFα polypeptide in the presence of compound to the degree of prolyl hydroxylation of the HIFα polypeptide in the absence of compound, wherein a decrease in the degree of prolyl hydroxylation in the presence of compound compared to the degree of prolyl hydroxylation in the absence of compound identifies a compound that decreases proline hydroxylation of HIFα.