Patent ID: 6640192
Filing Date: 2003-10-28
Classification: C12N,C40B

Abstract:
A protein or peptide comprising a peptide sequence encoded by a DNA sequence from a bank of genes, wherein said genes comprise a double-stranded DNA sequence which is represented by the following formula of one of their strands:5â€²B1B2B3 . . . BnXn+1 . . . Xn+aZn+a+1Zn+a+2Xn+a+3 . . . Xn+a+bQn+a+b+1 . . . Qn+a+b+j3â€²wherein n, a, b, and j are integers and are defined as: n>3, a>1, b>3 and j>1, â€ƒwherein Xn+1 . . . Xn+a+b is a hypervariable sequence and B represents any of bases adenine (A), cytosine (C), guanine (G) or thymine (T), X represents any of bases A, C, G or T, Z represents any of bases A, C, G or T, and Q represents any of bases A, C, G or T, wherein, (i) Z represents G or T at a G:T ratio of 1:1, and/or (ii) Z represents C or T at a C:T ratio of 1:1, and/or (iii) Z represents A or G at a A:G ratio of 1:1, and/or (iv) Z represents A or C at a A:C ratio of 1:1, and â€ƒwherein subsequences B1 . . . Bn and/or Qn+a+b+1 . . . Qn+a+b+j represent recognition sites for restriction enzymes, and wherein the recognition sites are oriented such that their cleavage site upon cleavage generates a cohesive end including the two bases designated Z.