Patent ID: 7612190
Filing Date: 2009-11-03
Classification: A61P,C12N,C12Q,G01N

Abstract:
1. An isolated nucleic acid encoding a fusion protein formed between a heterogeneous polypeptide and a polypeptide having an activity for targeting a heterogeneous polypeptide to a surface of cytoplasmic membrane, wherein the nucleic acid encoding said polypeptide having an activity for targeting a heterogeneous polypeptide to a surface of cytoplasmic membrane has a nucleotide sequence selected from the group consisting of the following (A) to (B): (A) a nucleotide sequence encoding an amino acid sequence selected from the group consisting of the following (a) to (b): (B) a nucleotide sequence of a nucleic acid that hybridizes to an antisense sequence of the nucleic acid consisting of the nucleotide sequence shown in SEQ ID NO: 1 under conditions of carrying out incubation at 65° C., overnight in a solution containing 6×SSC, 0.5% SDS, 5×Denhardt's, and 100 μg/ml herring sperm DNA, and carrying out washing using a buffer with 2×SSC at a temperature of 25° C. below a T