Patent ID: 6258564
Filing Date: 2001-07-10
Classification: A61P,C07K

Abstract:
A method for preparing antibodies reacting with cell surface antigens which are expressed by tryptase and chymase positive, human mast cells, wherein said antibodies are secreted by hybridoma clone ahMC5C12 (ATCC No. FERM BP-6070), wherein after human cord blood cells are injected to a newborn mammal, except human neonate, causing to ablate the induction ability of antibody production for all the antigens of said cells, tryptase and chymase positive human mast cells are injected to said mammal for immunization, hybridomas are prepared by fusing antibody producing cells obtained from said immunized mammal with myeloma cells, clones which are producing antibodies that react with said tryptase and chymase positive human mast cells are selected among said hybridomas, said selected clone is cultured and said antibodies are harvested by purifying the supernatant of said selected and cultured clone.