Patent ID: 7901910
Filing Date: 2011-03-08
Classification: C12N

Abstract:
1. A method for expressing an insoluble fusion protein of a heterologous protein and a carrier protein in an inclusion body of a prokaryotic host cell, comprising the steps of: (a) providing a DNA vector having: (b) transforming the host cell with the DNA vector; and, (c) expressing an insoluble fusion protein of the heterologous protein and the carrier protein, wherein the heterologous protein is joined at its N-terminus to the first domain of the carrier protein and at its C-terminus to the second domain of the carrier protein, where the first domain is sufficiently long to permit expression of the heterologous protein and the second domain is sufficiently long to permit separation, based on apparent molecular weight, of the fusion protein from expression products comprising some or all the heterologous protein but lacking the second domain, and wherein said fusion protein is located in an inclusion body of said host cell.