Patent ID: 9176063
Filing Date: 2015-11-03
Classification: C07K,G01L,G01N

Abstract:
1. A method of detecting a pressure or a change in pressure applied to a liquid containing: A. a modified fluorescent protein (MFP), B. a fusion fluorescent protein (FFP), wherein the FFP is a fusion fluorescent protein in which the MFP is fused with a fluorescent protein having an excitation spectrum different from an excitation spectrum of the MFP, the method comprising: detecting fluorescent intentsity of the MFP or FFP; and comparing the detected fluorescent intensity to (a) the fluorescent intensity of a control wild-type fluorescent protein or (b) the fluorescent intensity of the MFP or FFP when no pressure is applied, to determine the pressure or the change in pressure applied to the liquid containing the MFP or FFP, wherein when pressure applied to the liquid containing the MFP or FFP increases, the fluorescent intensity of the MFP or FFP increases, and when pressure applied to the liquid containing the MFP or FFP decreases, the fluorescent intensity of the MFP or FFP decreases.