Patent ID: 6514523
Filing Date: 2003-02-04
Classification: A61K,Y10T

Abstract:
A process for preparing a synthetic HDL spherical particle comprising at least one HDL apolipoprotein, at least one amphipathic lipid, and at least one hydrophobic active agent, said process consisting essentially of the steps of:(a) admixing at least one amphipathic lipid with at least one hydrophobic active agent in a solvent, said at least one hydrophobic active agent being selected from the group consisting of a hydrophobic drug, an amphipathic drug, and a lipid-soluble component; (b) removing said solvent to produce a dried mixture; (c) hydrating said dried mixture in an aqueous buffer to produce an aqueous mixture, said aqueous buffer being essentially free of detergent; (d) adding at least one HDL apolipoprotein to said aqueous mixture; and (e) vigorously mixing by sonication, trituration, or homogenization said HDL apolipoprotein and said aqueous mixture to form said synthetic HDL spherical particle.