Patent ID: 6555110
Filing Date: 2003-04-29
Classification: A61K,C07K,Y02A

Abstract:
A method of preparing an encapsulated composition, comprising the steps of:a) providing an aqueous phase of a solubilized amount of an antibody or protein material to be encapsulated, said material being solubilized in phosphate buffered saline; b) mixing said solubilized material with an amount of biodegadable and nonantigenic albumin dissolved in water or phosphate buffered saline to form an aqueous phase mixture; c) adding said aqueous phase mixture of step b) to an amount of olive oil; d) emulsifying said aqueous phase mixture formed in step c) to form microspheres of encapsulated material; and, e) counting said encapsulated microspheres with a crosslinking agent for a time sufficient to crosslink at least a portion of said microspheres, such that said crosslinked microspheres have bioactivity and such that denaturization is substantially prevented.