Patent ID: 9034646
Filing Date: 2015-05-19
Classification: A61K,A61P,C12N

Abstract:
1. A method for preparing a virally-inactivated growth factors-containing platelet lysate depleted of PDGF and VEGF comprising: a) containing a starting platelet concentrate with a solvent and a detergent; b) incubating for a period of at least 5 minutes to 6 hours, at a physiological pH or at a pH ranging from pH 6.8 to 8.2, and at a temperature within the range of from 2° C. to 50° C.; c) removing the solvent and detergent by oil extraction and obtaining an aqueous protein phase; d) mixing activated charcoal with the aqueous protein phase obtained in c); e) centrifuging the mixture of d) to pellet the charcoal; and f) recovering the supernatant from e), wherein the supernatant is virally-inactivated and depleted of PDGF and VEGF.