Patent ID: 6927061
Filing Date: 2005-08-09
Classification: A61K,A61P,C12N,G01N

Abstract:
1. A method of isolating a substantially pure population of ovarian mesothelial cells, comprising: (a) microdissecting a source of human fetal ovarian mesothelial cells; (b) placing the source of ovarian mesothelial cells in nutrient media under culture conditions sufficient to sustain life of said ovarian mesothelial cells and wherein the nutrient media contains nutrients consisting of insulin, transferrin, epidermal growth factor, α-tocopherol, recombinant human heregulin β1, bovine serum albumin, and aprotinin; (c) maintaining suitable culture conditions sufficient to allow the migration of ovarian mesothelial cells from the source of ovarian mesothelial cells into the nutrient media; (d) maintaining suitable culture conditions sufficient to allow ovarian mesothelial cells to form aggregate or monolayer formations; and (e) subculturing said aggregate or monolayer formations to obtain a substantially pure population of ovarian mesothelial cells.