Patent ID: 7235233
Filing Date: 2007-06-26
Classification: A61K,C07K,C12N

Abstract:
1. A method of delivering a nucleic acid of interest to a human skeletal muscle cell in vitro or a human myoblast cell in vitro, the method comprising: delivering the nucleic acid of interest to said human skeletal muscle cell or said human myoblast cell by infecting said human skeletal muscle cell in vitro or said human myoblast cell in vitro with a replication-deficient recombinant serotype 5 adenovirus, said recombinant serotype 5 adenovirus having a tropism for said human skeletal muscle cell or said human myoblast cell, said recombinant serotype 5 adenovirus comprising an adenoviral genomic nucleic acid comprising the nucleic acid of interest; wherein said recombinant serotype 5 adenovirus comprises a fiber protein comprising a knob and a shaft region from the fiber protein of an adenovirus serotype selected from the group consisting of serotypes 11, 13, 16, 17, 35, 38, 40-S, 40-L, 47 and 51.