Patent ID: 8546144
Filing Date: 2013-10-01
Classification: C12N,G01N,Y10T

Abstract:
1. A method of making a storage stable control for determining glycated hemoglobin comprising: contacting intact non-diabetic red blood cells in a suspension medium with mannose under conditions such that the concentration of S-A1c glycated hemoglobin is increased to greater than about 6 percent by weight of the hemoglobin in the intact red blood cells; treating the red blood cells with a fixative agent so that the red blood cells' membranes are preserved and hemoglobin leakage from the red blood cells is substantially eliminated; and adding to the suspension medium an effective amount of one or more of sorbitol, fructose, and mannitol effective to provide for consistent results when measuring hemoglobin that is glycated or acetylated or both as determined by HPLC, immunoassay, or boron affinity methods of analysis; and forming a hematology control composition with the red blood cells.