Patent ID: 6383393
Filing Date: 2002-05-07
Classification: C12N

Abstract:
A method for the purification and separation of a nucleic acid mixture by chromatography, comprising the steps of:a) adsorbing on a substrate the nucleic acid mixture from an aqueous adsorption solution containing (i) salts effecting a high ionic strength and (ii) 1 to 50% by volume of at least one C1-C5 aliphatic alcohol or polyethylene glycol or at least one C1-C5 aliphatic alcohol and polyethylene glycol wherein said substrate comprises a porous or non-porous mineral substrate selected from the group consisting of silica gel, glass fibers, quartz fibers, and zeolites, followed by b) optionally washing said substrate with a washing solution; followed by c) eluting said nucleic acid mixture with a solution having a lower ionic strength than the aqueous adsorption solution, effecting thereby a nucleic-acid fraction; and d) collecting the nucleic-acid fraction.