Patent ID: 8691960
Filing Date: 2014-04-08
Classification: C07K,C12N

Abstract:
1. A polynucleotide comprising a nucleotide sequence encoding a polypeptide comprising a functional (R)-2-hydroxyacid dehydrogenase useful for catalyzing the enantioselective conversion of a 1-carboxy-2-ketoacid to a 1-carboxy-(R)-2-hydroxyacid, or the reverse reaction, wherein the encoded polypeptide is at least 90% identical to the amino acid sequence of SEQ ID NO: 2, with the proviso that the encoded polypeptide comprises arginine 143 substituted with histidine or cysteine; and wherein the R143H or R143C substitution disrupts oxidative decarboxylation activity but does not disrupt oxidoreductase activity.