Patent ID: 8815538
Filing Date: 2014-08-26
Classification: A61K,A61Q,C12Q,G01N

Abstract:
1. A method of making a topical cosmetic composition, the method comprising: (a) providing a first culture comprising a test agent, a quantity of a human skin commensal microorganism, and a minimal carbon medium; (b) determining a metabolite level or a replication level of the human skin commensal microorganism of the first culture; (c) comparing the metabolite level or the replication level to a first control value, wherein the first control value is the metabolite level or replication level in a culture of the human skin commensal microorganism of the first culture that is grown without the test agent, and wherein the metabolite level correlates with the amount of growth; (d) identifying the test agent as a prebiotic when the test agent increases the growth of the human skin commensal microorganism and when the metabolite level or replication level of the first culture is greater than the first control value; and (e) combining a safe and effective amount of the prebiotic with a dermatologically acceptable carrier.