Patent ID: 6841370
Filing Date: 2005-01-11
Classification: A23K,C12N,C12Y

Abstract:
1. An isolated DNA molecule encoding a mutant phytase produced by making a plurality of amino acid substitutions in a wild-type Escherichia coli phytase having an amino acid sequence of SEQ ID NO:1, said amino acid substitutions comprising substitutions at positions 200, 207, and 211, wherein the DNA molecule comprises a nucleotide sequence of SEQ ID NO:4 or hybridizes to a complement of the nucleotide sequence of SEQ ID NO:4 under stringency conditions comprising hybridization at 42° C. in hybridization medium comprising 5×SSPE and 50 percent formamide with washing at 65° C. with 0.5×SSPE, and wherein the mutant phytase has phytase activity.