Patent ID: 9174216
Filing Date: 2015-11-03
Classification: B01L,C12Q,G01N

Abstract:
1. A system for capturing and analyzing a target cell population from a biological sample, comprising: a fluid delivery module including a cylindrical cartridge having a set of chambers that hold at least one fluid for processing the biological sample and an actuator coupled to a piercer configured to access at least one chamber of the set of chambers; a reservoir, including a reservoir inlet and a reservoir outlet, configured to receive the biological sample and to receive at least one fluid from the fluid delivery module at the reservoir inlet, wherein the cylindrical cartridge of the fluid delivery module is rotatable about an axis to align a chamber of the set of chambers with the piercer, to facilitate flow of contents of the chamber into the reservoir inlet, and wherein the set of chambers is angularly displaced about the axis; a manifold coupled to the reservoir at the reservoir outlet and configured to receive and deliver the biological sample and at least one fluid from the reservoir into a cell capture device; a waste chamber coupled to the manifold and configured to receive waste fluid from the manifold; a pump coupled to at least one of the waste chamber, the manifold, and the waste chamber and configured to drive fluid flow within the manifold; and a magnet proximal the reservoir, wherein, in a first configuration, the pump drives flow of the biological sample from the reservoir in a forward direction and the magnet retains a concentration of contaminating particles from the biological sample at the reservoir, thereby facilitating separation of the target cell population from other constituents of the biological sample.