Patent ID: 8058053
Filing Date: 2011-11-15
Classification: C12N,C12P

Abstract:
1. A genetically engineered Pichia strain which produces glycoproteins comprising a core N-glycan structure characterized by the formula, GalGlcNAcMan5 or fewer GlcNAc2, wherein said strain expresses (1) an α-1,2-mannosidase or an enzymatically active fragment thereof which converts Man8GlcNAc2 to Man5GlcNAc2, (2) an N-acetylglucosaminyltransferase I (or GnTI) or an enzymatically active fragment thereof which converts Man5GlcNAc2 to GlcNAcMan5GlcNAc2, and (3) a β-1,4-galactosyltransferase (GalT) or an enzymatically active fragment thereof, wherein the genomic OCH1 gene of said strain is inactivated, and wherein at least about 10% of GlcNAcMan5GlcNAc2 is converted to GalGlcNAcMan5GlcNAc2.