Patent ID: 6242192
Filing Date: 2001-06-05
Classification: C12N,C12Y

Abstract:
A method of expressing a gene of interest in a eukaryotic host cell, said method comprising:(a) introducing into said eukaryotic host cell a recombinant DNA vector that contains a viral enhancer, a eukaryotic promoter, and a DNA sequence that encodes said gene of interest, said sequence being positioned for expression from said promoter, and incorporating DNA that encodes the first part of the tripartite leader of an adenovirus (SEQ ID NO:1) into said vector such that, upon transcription, the mRNA produced encodes said useful product and, at the '5 end, contains a first part of said tripartite leader subject to the limitation that said mRNA contains only the first part of the tripartite leader of said adenovirus;(b) culturing said eukaryotic host cell transformed in step (a) under conditions that allow expression of said DNA sequence and expression of the gene of interest, wherein said transformed host cell also expresses the VA gene product.