Patent ID: 7214484
Filing Date: 2007-05-08
Classification: C12N

Abstract:
1. A kit for extracting nucleic acids from tissue samples for polymerase chain reaction (PCR) amplification, wherein the tissue sample is incubated in an extraction composition for not more than 60 minutes, the kit comprising components of a nucleic acid extraction composition, packaged separately or in one or more mixtures of any combination thereof, each separate component or mixture being in a different container for mixing prior to use, the components of the extraction composition comprising a protease enzyme, a buffering component which maintains the pH at 7.5 or greater in the extraction composition and a Ca 2+ -chelator which produces a concentration of not more than about 100 mM of said Ca 2+ -chelator in the extraction composition upon mixing the components, wherein the components of the extraction composition do not include a surface active agent.