Patent ID: 7968327
Filing Date: 2011-06-28
Classification: C12N,C12Q,Y10T

Abstract:
1. A method of collecting a nucleic acid from formalin fixed paraffin embedded cells, said method comprising: suspending the formalin fixed paraffin embedded cells in an aqueous solution; incubating the suspension 3 hours or more at a temperature ranging from 40° C. to 100° C., and at least 2° C. below the Tm for double stranded DNA of the cells, whereby the paraffin melts and the nucleic acid is released from the cells into the aqueous solution, wherein the suspended cells incubated in the suspension are other than dewaxed cells; and, physically separating the aqueous solution from the paraffin after said incubation, thereby collecting the nucleic acid from the cells; wherein the paraffin has a melting point 71° C. or less and greater than 40° C.