Patent ID: 7354756
Filing Date: 2008-04-08
Classification: C07K,C12N,G01N

Abstract:
1. An isolated nucleic acid molecule encoding a polypeptide comprising a first carboxy-terminal portion of a split intein (C-intein), a second amino-terminal portion of a split intein (N-intein), and a target peptide flanked on one end with the carboxy-terminal portion of a split intein (C-intein) and on its other end with the amino-terminal portion of a split intein (N-intein); wherein expression of the nucleic acid molecule in a host system produces the polypeptide in a form selected from the group consisting of: (a) a polypeptide that spontaneously splices in the host system to yield a cyclized form of the target peptide, and (b) a splicing intermediate of a cyclized form of the target peptide.