Patent ID: 8206898
Filing Date: 2012-06-26
Classification: C12N,C12Q,G01N

Abstract:
1. A method for identifying an agent that binds to a homologous RNA-exit-channel amino-acid sequence of a bacterial RNAP, comprising the steps of: (a) preparing a reaction solution including the agent to be tested and a bacterial RNAP that contains a homologous RNA-exit-channel amino-acid sequence; (b) detecting at least one of the presence, extent, concentration-dependence, or kinetics of binding of the agent to the homologous RNA-exit-channel amino-acid sequence of the bacterial RNAP, and (c) using the information obtained in step (b) to identify an agent that binds to the homologous RNA-exit-channel amino-acid sequence of the bacterial RNAP, wherein the agent is not lipiarmycin.