Patent ID: 8486420
Filing Date: 2013-07-16
Classification: A61K,A61P,C12N

Abstract:
1. A self-propagating chimeric viral vector comprising a modified alphavirus genome, said modified genome comprising: (a) alphavirus nsp1, nsp2, nsp3 and nsp4 coding sequences and alphavirus cis-acting sequences comprising alphavirus 5′ and 3′ untranslated sequences sufficient for replication of the modified alphavirus genome; (b) lentivirus protease coding sequences; and (c) lentivirus structural protein coding sequences necessary to form a virion, wherein the lentivirus structural protein coding sequences comprise lentivirus capsid, matrix and envelope coding sequences; and wherein the lentivirus is a human immunodeficiency virus (HIV), a simian immunodeficiency virus (SIV) or a SIV/HIV chimera (SHIV); wherein at least one of the lentivirus structural proteins encoded by the lentivirus structural protein coding sequences is modified to incorporate a nucleic acid binding domain that binds to the modified alphavirus genome; and wherein the combination of the alphavirus cis-acting sequences and the expression of the coding sequences of (a), (b) and (c) are sufficient to form a self-propagating virion that packages the modified alphavirus genome.