Patent ID: 8497119
Filing Date: 2013-07-30
Classification: C12M,C12N

Abstract:
1. A method for performing micro-operations on a vesicular object by: a) securing the vesicular object by applying negative pressure in a radial vacuum passage ( b) advancing a pipette ( c) penetrating the secured vesicular object at the secondary aperture ( d) ensuring that the axial vacuum passage and the radial vacuum passage are not in fluid contact with each other or with atmospheric pressure; e) controlling the negative pressure in both axial and radial vacuum passages, and the pressure in the pipette, separately; whereby allowing the simultaneous holding of the vesicular object at the primary aperture by applying negative pressure in the radial vacuum passage; injection into or aspiration from the vesicular object via the pipette; and aspiration from the vesicular object at the secondary aperture by applying negative pressure in the axial vacuum passage.