Patent ID: 7638299
Filing Date: 2009-12-29
Classification: A61P,C07K,C12N

Abstract:
1. A method for making a biosynthetic polypeptide (BSP) less than 100 amino acids in length comprising two or more non-naturally encoded amino acids wherein said BSP is a (glucagon like polypeptide 1 (GLP-1) comprising; expressing an mRNA encoding said GLP-1 peptide in a translation system, wherein said mRNA comprises two or more selector codons, and said translation system further comprises an orthogonal aminoacyl tRNA synthetase (O-RS) and an orthogonal tRNA (O-tRNA) wherein said O-RS preferentially aminoacylates said O-tRNA with said non-naturally encoded amino acids, and said O-tRNA recognizes the two or more selector codons on the mRNA and wherein at least two of the non-naturally encoded amino acids are linked together.