Patent ID: 6511798
Filing Date: 2003-01-28
Classification: C12N,Y10S

Abstract:
A method for preparation of a differentiated cell monolayer, useful for study of drug transport, which method comprises seeding a permeable, charged polymeric support with confluent cells of human adenocarcinoma cell line CaCo-2 in a growth medium, incubating the support to allow cell attachment, and replacing the growth medium about one day and again about 5 days after initial incubation, wherein a maturation agent is added to the growth medium about 5 days after seeding, whereby cells grow on the support and form a differentiated cell monolayer in about seven days, wherein said monolayer is characterized by permeability to mannitol in a range of about 4-8Ã—10âˆ’7 cm/s.