Patent ID: 7892830
Filing Date: 2011-02-22
Classification: C12N

Abstract:
1. A method of creating a clonal culture of human pluripotent stem cells comprising the steps of: providing a culture of human pluripotent stem cells in culture in a fibroblast growth factor-containing culture medium in a culture vessel; selecting a single pluripotent stem cell from the stem cells in culture; initiating a new culture of pluripotent stem cells with the single pluripotent stem cell in a culture vessel containing an extracellular matrix coating a surface inside the culture vessel and a fibroblast growth factor-containing culture medium, the culture medium including an agent selected to increase the cloning efficiency of the pluripotent stem cell culture relative to a pluripotent stem cell culture that does not contain the agent, the agent being a small molecule having the formula: wherein R culturing the single pluripotent stem cell to create a clonal culture of human pluripotent stem cells.