Patent ID: 6617106
Filing Date: 2003-09-09
Classification: C07H,C12N,C12P,C12Q,Y10S

Abstract:
A method of making an oligonucleotide, the method comprising:providing a template oligonucleotide comprising a sequence of nucleotides, the template comprising at least one non-standard nucleotide at a preselected site in the sequence; contacting the template with a mixture of nucleotide triphosphates, the mixture comprising nucleotide aiphosphates that are complementary to the nucleotides of the template, wherein the nucleotide triphosphate complementary to the non-standard nucleotide at the preselected site comprises a detivatized nucleotide; and forming an oligonucleotide complementary to a portion of the template by enzymatic polymerization of the nucleotide triphosphates in a sequence complementary to the portion of the template, wherein the non-standard nucleotide at the preselected site is iso-G or iso-C.