Patent ID: 6140111
Filing Date: 2000-10-31
Classification: A61K,A61L,C12N

Abstract:
A recombinant retroviral vector, said vector comprising in operable combinationa) 5' R and U5 sequences, and 3' U3 and R sequences derived from the LTRs of a retrovirus of interest;b) a Psi packaging site recognized by an amphotropic retrovirus;c) an insertion site for a gene of interest located 3' to said U5 sequence; andd) a splice acceptor site located 3' to said Psi packaging site, wherein said splice acceptor site is a splice acceptor site necessary for the generation of the env mRNA of a wild type retrovirus;wherein said recombinant retroviral vector does not contain a complete gag, pol, or env gene, and does not express a functional marker gene.