Patent ID: 6297371
Filing Date: 2001-10-02
Classification: A61P,B01D,C12N,Y10S,Y10T

Abstract:
In a method comprising linking therapeutically active nucleic acids, oligonucleotides, or combination thereof to a transfection vector, the improvement wherein the therapeutically active nucleic acids, oligonucleotides, or combination therefor are obtained from a biological source of raw cells by a method comprising, the steps of:(a) lysing the raw cells in said biological source to obtain a raw fraction of the nucleic acids, oligonucleotides, or combination thereof;(b) applying said raw fraction on an anion exchanger, which anion exchanger begins desorbing DNA and RNA at different ionic strengths, the ionic strength at which DNA begins desorbing corresponding to a sodium chloride solution having a concentration higher by at least 100 mM than the concentration of a sodium chloride solution corresponding to the ionic strength at which RNA begins desorbing, thereby isolating the nucleic acids, oligonucleotides, or combination, thereof;(c) eluting the nucleic acids, oligonucleotides, or combination, thereof, from the anion exchanger to obtain a separated fraction of the nucleic acids, oligonucleotides, or combination, thereof; and(d) treating after either step (a) or step (c), the nucleic acids or oligonucleotides with non-ionic detergents, affinity chromatographic supports, or inorganic chromatographic material, thereby effecting removal of endotoxinis.