Patent ID: 9005950
Filing Date: 2015-04-14
Classification: C11D,C12N

Abstract:
1. A variant of a parent enzyme having pectate lyase activity (EC 4.2.2.2), comprising an alteration at one or more positions selected from the group consisting of 31, 48, 106, 140, 215, 305, 378, and 391, wherein the alteration(s) are independently (i) an insertion of an amino acid downstream of the amino acid which occupies the position, (ii) a deletion of the amino acid which occupies the position, or (iii) a substitution of the amino acid which occupies the position with a different amino acid, and wherein each position corresponds to a position of the amino acid sequence of the pectate lyase having the amino acid sequence of SEQ ID NO: 2, and wherein the variant has at least 98% identity to the amino acid sequence of SEQ ID NO: 2.