Patent ID: 6051388
Filing Date: 2000-04-18
Classification: G01N,Y10S

Abstract:
A biological assay material for detecting the presence of a particular toxic substance comprising:a base layer which is a flexible polyoletin film having a surface which has undergone a treatment step effective to enhance said film's ability to immobilize a ligand applied thereto;a capture antibody which is a biologically active ligand characterized by its ability to recognize an epitope of the particular toxic substance, said ligand being immobilized onto said surface of said polyolefin film;a first agarose gelcoat layer overlying the capture antibody, said agarose layer being permeable to the toxic substance and containing ingredients to enhance the growth thereof, said layer further containing a detector antibody which is a biologically active ligand characterized by its ability to recognize a different epitope of said particular toxic substance, thereby forming a detector antibody/antigen complex; anda second protective gelcoat layer overlying the detector antibody and having a degree of porosity whereby passage of said toxic substance is permitted and passage of said detector antibody/antigen complex is prevented, said second protective gelcoat layer having a degree of abrasion resistance effective to protect the biological assay material.