Patent ID: 6518027
Filing Date: 2003-02-11
Classification: B01L

Abstract:
A sample preparation method for DNA analysis, comprising the steps of:digesting a plurality of DNA samples, separately, with a restriction enzyme to generate a plurality of DNA fragments originating from each of said DNA samples; ligating an oligonucleotide into the 3â€²-end of each of said DNA fragments, wherein each said oligonucleotide has a known base sequence comprising a common base sequence that is the same for and common to all of said DNA fragments at the 3â€²-end and a discriminating base sequence that discriminates the DNA samples by length following the 5â€²-end of said common base sequence to discriminate said DNA samples, the length of said discriminating base sequence is the same for the DNA fragments originating from one specific DNA sample of said DNA samples but varies for each of said DNA samples, and the length of said common base sequence is the same for said DNA fragments originating from any of said DNA samples; amplifying said DNA fragments by PCR (polymerase chain reaction) in a single reaction cell by using a plurality of specific primers and a free primer, as primers, wherein each of said specific primers has a base sequence complementary to a part of the base sequence of all of said DNA fragments and hybridizes with the part of base sequence of all of said DNA fragments to be amplified, said specific primers are immobilized on the surfaces of a plurality of supports which are mutually separable, by hybridization between said specific primers immobilized on the plurality of different supports and each of said DNA fragments, into a plurality of groups according to the base sequences of said specific primers, said free primer is contained in a polymerase chain reaction solution in said single reaction cell, and the amplified DNA fragments by said PCR are produced on the surfaces of said supports in mutually isolated places; separating and recovering said supports on which the PCR amplification products are produced, for each of said mutually separable groups, according to a physical property of said supports; and recovering, separately, the PCR amplification products on the surfaces of said supports, for each of said mutually separable groups.