Patent ID: 8338175
Filing Date: 2012-12-25
Classification: A61L,C12N

Abstract:
1. A method for generating a conjunctival tissue system consisting essentially of obtaining a conjunctival biopsy obtained from the fornix region of the conjunctiva, the conjunctival biopsy comprising a stroma segment and an epithelial segment, removing the stroma segment of the conjunctival biopsy, and culturing the epithelial segment of the conjunctival biopsy on a support material in a single medium comprising: (a) Dulbecco's Modified Essential Medium (DMEM), DMEM:F-12 (1:1) medium, or Ham's F-12 (1:1) medium; (b) a nutrient serum selected from the group consisting of knock-out serum, heat-inactivated human serum, human cord blood serum, human serum albumin, and fetal bovine serum; (c) epidermal growth factor (EGF); (d) basic fibroblast growth factor (bFGF); (e) insulin; (f) sodium selenite; (g) transferrin; and (h) an antibiotic or mixture of antibiotics, wherein the single medium supports the culture and differentiation of the conjunctival biopsy on the support material to form a conjunctival tissue system.