Patent ID: 6200791
Filing Date: 2001-03-13
Classification: A61P,C12N,Y10S

Abstract:
A method of purifying a thrombin-like protease from snake venom selected from the group consisting of ancrod and strongly basic proteases, which method comprisesa) obtaining a solution comprising the protease;b) loading the protease solution onto an affinity chromatography matrix or an anion exchange resin;c) eluting the protease in an eluate solution from the affinity chromatography matrix or anion exchange resin;d) loading the eluate solution from step c) onto a matrix of glass beads; said glass beads having a pore diameter of from 25 to 35 nm and a particle size of from 30 to 60 .mu.m; said eluate being applied to the matrix of glass beads in a basic solution at a pH value of from 7.5 to 9.0 whereby the glass beads adsorb the thrombin-like protease from snake venom;e) eluting the protease in an eluate solution from the matrix of glass beads;f) loading the eluate solution from step e) onto a size exclusion gel matrix or onto a matrix of glass beads; said glass beads having a pore diameter of from 25 to 35 nm and a particle size of from 30 to 60 .mu.m; said eluate being applied to the matrix of glass beads in an acidic solution; andg) eluting and recovering the purified protease.