Patent ID: 9217169
Filing Date: 2015-12-22
Classification: C07K,C12N,C12P

Abstract:
1. A genetically manipulated microorganism that comprises a recombinant nucleic acid molecule encoding an amino acid sequence having enzymatic activity of synthetic pathway enzymes for the production of Argyrins, wherein the amino acid sequence comprises a combination of: (a) the amino acid sequences encoded by nucleotides 1608 to 4 of SEQ ID NO: 1 (orf1), by nucleotides 3615 to 1687 of SEQ ID NO: 1 (orf2), by nucleotides 5139 to 3661 of SEQ ID NO: 1 (orf3), by nucleotides 7388 to 5274 of SEQ ID NO: 1 (orf4), by nucleotides 7710 to 8048 of SEQ ID NO: 1 (orf5), by nucleotides 8870 to 8043 of SEQ ID NO: 1 (orf6), by nucleotides 9293 to 10282 of SEQ ID NO: 1 (orf7), and by nucleotides 11057 to 10320 of SEQ ID NO: 1 (orf8); (b) the amino acid sequences of SEQ ID NO: 7 (Arg1), of SEQ ID NO: 8 (Arg2) of SEQ ID NO: 9 (Arg3), of SEQ ID NO: 10 (Arg4), and of SEQ ID NO: 11 (Arg5); and (c) the amino acid sequences encoded by nucleotides 45620 to 44706 of SEQ ID NO: 1 (orf9), by nucleotides 46507 to 45617 of SEQ ID NO: 1 (orf10), by nucleotides 47244 to 46504 of SEQ ID NO: 1 (orf11), by nucleotides 47547 to 47975 of SEQ ID NO: 1 (orf12), by nucleotides 48288 to 49268 of SEQ ID NO: 1 (orf13), by nucleotides 49483 to 55209 of SEQ ID NO: 1 (orf14), and by nucleotides 55212 to 55565 of SEQ ID NO: 1 (orf15).