Patent ID: 8216851
Filing Date: 2012-07-10
Classification: G01N,G01R,Y10T

Abstract:
1. A method for characterizing a mixture of N-glycans by NMR comprising steps of: providing a sample that includes a mixture of N-glycans; (i) the structural characteristic is an oligomannose structure and the step of identifying comprises determining whether the sample produces a (ii) the structural characteristic is a GlcNAc or sialic acid residue and the step of identifying comprises determining whether the sample produces a (iii) the structural characteristic is a sialic acid residue and the step of identifying comprises determining whether the sample produces a (iv) the structural characteristic is a sialic acid residue with an α2-3 linkage and the step of identifying comprises determining whether the sample produces a (v) the structural characteristic is a sialic acid residue with an α2-6 linkage and the step of identifying comprises determining whether the sample produces a (vi) the structural characteristic is di- or tri-acetylated NeuAc and the step of identifying comprises determining whether the sample produces a (vii) the structural characteristic is a fucose residue and the step of identifying comprises determining whether the sample produces a (viii) the structural characteristic is a sialic acid and the step of identifying comprises determining whether the sample produces a (ix) wherein the structural characteristic is a mono-antennary Man4 residue and the step of identifying comprises determining whether the sample produces a (x) the structural characteristic is a bi-antennary Man4 residue and the step of identifying comprises determining whether the sample produces a (xi) the structural characteristic is a galactose residue in a lactosamine extension and the step of identifying comprises determining the chemical shifts of a (xii) the structural characteristic is a sulfated GlcNac residue and the step of identifying comprises determining the chemical shifts of a (xii) the structural characteristic is a phosphorylated mannose residue and the step of identifying comprises determining the chemical shifts of a (xiv) the structural characteristic is a core fucose residue and the step of identifying comprises determining the chemical shifts of a (xv) the structural characteristic is a sialic acid residue and the step of identifying comprises determining whether the sample produces a (xvi) the structural characteristic is a sialic acid residue and the step of identifying comprises determining whether the sample produces a (xvii) the structural characteristic is an acetylated sialic acid residue and the step of identifying comprises determining the (xviii) the structural characteristic is a Man4 residue and the step of identifying comprises determining the chemical shifts of a (xix) the structural characteristic is a mono- or bi-antennary Man4 residue and the step of determining comprises determining whether the Man4 residue is mono-antennary or bi-antennary based on the chemical shifts of the (xx) the structural characteristic is a Man4′ residue and the step of identifying comprises determining whether the sample produces a (xxi) the structural characteristic is a mono- or bi-antennary Man4′ residue and the step of determining comprises determining whether the Man4′ residue is mono-antennary or bi-antennary based on the chemical shifts of the (xxii) the structural characteristic is a GlcNAc1 residue and the step of identifying comprises determining whether the sample produces a (xxiii) the structural characteristic is a GlcNAc2 residue and the step of identifying comprises determining whether the sample produces a (xxiv) the structural characteristic is a Man3 residue and the step of identifying comprises determining whether the sample produces a (xxv) the structural characteristic is a GlcNac residue with a β(1-2) linkage to mannose and the step of identifying comprises determining whether the sample produces a (xxvi) the structural characteristic is a GlcNAc residue with a β(1-4) or β(1-6) linkage to mannose and the step of identifying comprises determining whether the sample produces a (xxvii) the structural characteristic is a GlcNAc residue in a lactosamine extension and the step of identifying comprises determining whether the sample produces a (xxviii) the structural characteristic is an unsubstituted galactose residue and the step of identifying comprises determining whether the sample produces a (xxix) the structural characteristic is a galactose residue with an α(2-3) sialic acid attached and the step of identifying comprises determining whether the sample produces a (xxx) the structural characteristic is a galactose residue with an α(2-6) sialic acid attached and the step of identifying comprises determining whether the sample produces a (xxxi) the structural characteristic is a galactose residue in a lactosamine extension and the step of identifying comprises determining whether the sample produces a (xxxii) the structural characteristic is an oligomannose structure and the step of identifying comprises determining whether the sample produces a (xxxiii) the structural characteristic is a core fucose residue and the step of identifying comprises determining whether the sample produces a (xxxiv) the structural characteristic is a core fucose residue and the step of identifying comprises determining whether the sample produces a (xxxv) the structural characteristic is an antennary fucose residue and the step of identifying comprises determining whether the sample produces a (xxxvi) the structural characteristic is a label attached to GlcNAc1 and the step of identifying comprises determining whether the sample produces a (xxxvii) the structural characteristic is a label attached to GlcNAc1 and the step of identifying comprises determining whether the sample produces a split (xxxviii) the structural characteristic is a label attached to GlcNAc1 and the step of identifying comprises determining whether the sample produces a (xxxix) the structural characteristic is a label attached to GlcNAc1 and the step of identifying comprises determining the chemical shifts of a (xxxx) the structural characteristic is a label attached to GlcNAc1 and the step of identifying comprises determining whether the sample produces a split (xxxxi) the structural characteristic is a label attached to GlcNAc1 and the step of identifying comprises determining whether the sample produces a split determining whether the sample does or does not include an N-glycan with the structural characteristic in light of the step of identifying.