Patent ID: 8076063
Filing Date: 2011-12-13
Classification: C12Q

Abstract:
1. A method for multiplex detection of methylation in a population of double-stranded target nucleic acids comprising: (a) providing a population of double-stranded target nucleic acids labeled with a purification tag, wherein said target nucleic acids comprise potentially methylated target sequences; (b) cleaving said population of target nucleic acids with an enzyme, whereby said enzyme selectively cleaves at unmethylated target sequences in said population of target nucleic acids and does not cleave at methylated target sequences in said population of target nucleic acids, forming a population of cleaved target nucleic acids labeled with a purification tag and a population of non-cleaved target nucleic acids labeled with a purification tag; (c) immobilizing said population of non-cleaved said target nucleic acids by said purification tag, thereby forming immobilized non-cleaved target nucleic acids; and (d) detecting the presence of said immobilized non-cleaved target nucleic acids wherein said detecting step comprises: