Patent ID: 6670148
Filing Date: 2003-12-30
Classification: A61P,C07K

Abstract:
A composition comprising a polypeptide rendered atoxic by the prevention of formation of disulfide bonds in its tertiary structure prepared according to a method comprising the steps of:1) identifying a toxic polypeptide having a biological activity dependent on the presence of one or more disulfide bonds in its tertiary structure, 2) preparing a cDNA strand encoding the polypeptide, 3) expressing the cDNA in a Pichia expression system under conditions in which the polypeptide is produced in the cytoplasm in an inactive form due to the failure to form one or more disulfide bridges, and, 4) recovering the inactive polypeptide from the cytoplasm and formulating it into an aqueous composition without the formation of disulfide bridges.