Patent ID: 8546095
Filing Date: 2013-10-01
Classification: C12Q,G01N,Y10T

Abstract:
1. A method for determining the response on calcineurin activity of a human or animal subject to an immunosuppressive therapeutic agent, the method comprising: (i) obtaining an isolated population of T cells from a human or animal subject in receipt of an immunosuppressive therapeutic agent; (ii) lysing the isolated population of T cells; (iii) dividing the lysed isolated T cell population into at least a first aliquot and a second aliquot; (iv) determining a first level of calcineurin activity in the first aliquot of lysed T cells; (v) contacting the second aliquot of lysed T cells with a composition comprising a T cell receptor agonist and then determining a second level of calcineurin activity in the second aliquot of lysed T cells; and (vi) comparing the first and the second levels of calcineurin activity as determined in steps (iv) and (v) respectively, whereby the ratio of the first and the second levels of calcineurin activity indicates the degree of responsiveness on calcineurin activity by the human or animal subject to the immunosuppressive therapeutic agent;