Patent ID: 6261804
Filing Date: 2001-07-17
Classification: C07B,C07K,C12N,C40B,G01N

Abstract:
A method for producing a protein library, comprising the steps of:a) providing a population of RNA molecules, each of which comprises a translation initiation sequence and a start codon operably linked to a protein coding sequence and each of which is covalently bonded to a peptide acceptor at the 3' end of said protein coding sequence, said peptide acceptor being a molecule that can be added to the C-terminus of a growing protein chain by the catalytic activity of a ribosomal peptidyl transferase; andb) in vitro translating said protein coding sequences of said RNA molecules and then incubating said translation reaction under high salt conditions to produce a population of RNA-protein fusions, thereby producing a protein library.