Patent ID: 8822165
Filing Date: 2014-09-02
Classification: C07K,C12P,G01N

Abstract:
1. A method for producing cAMP using a chimeric olfactory receptor, the method comprising steps of: (a) preparing a reaction system comprising a first layer, a lipid bilayer membrane, and a second layer; wherein the lipid bilayer membrane is interposed between the first layer and the second layer, the lipid bilayer membrane comprises the chimeric olfactory receptor and adenylate cyclase; the chimeric olfactory receptor penetrates the lipid bilayer membrane; the adenylate cyclase penetrates the lipid bilayer membrane; the second layer contains ATP and a G protein; the G protein is placed in the vicinity of one end of the chimeric olfactory receptor; the chimeric olfactory receptor is derived from a mouse olfactory receptor consisting of an amino acid sequence of (N-terminal)—an EC1 domain—a TM1 domain—an IC1 domain—a TM2 domain—an EC2 domain—a TM3 domain—an IC2 domain—a TM4 domain—an EC3 domain—a TM5 domain—an IC3 domain—a TM6 domain—an EC4 domain—a TM7 domain—an IC4 domain—(C-terminal); the N-terminal of the chimeric olfactory receptor is modified with an amino acid sequence of Rho tag (SEQ ID NO:01)—myc epitope tag (SEQ ID NO:04); and the IC4 is substituted with an IC4 domain of a beta-1 adrenergic receptor; and (b) supplying a chemical substance which stimulates the chimeric olfactory receptor to the first layer so as to produce the cAMP from the ATP.