Patent ID: 6770456
Filing Date: 2004-08-03
Classification: A61K,A61P,C07K,G01N

Abstract:
An isolated nucleic acid molecule selected from the group consisting of:(a) nucleic acid molecules which fully hybridize under stringent conditions to a nucleic acid molecule having a nucleotide sequence set forth as SEQ ID NO:8, wherein the hybridizing nucleic acid molecule comprises a nucleotide sequence that encodes the amino acid sequence consisting of SEQ ID NO:9, and which code for a gene product that provokes a cytotoxic T cell immune response against a melanoma cancer cell that expresses the nucleic acid molecule, (b) nucleic acid molecules that differ from the nucleic acid molecules of (a) in codon sequence due to the degeneracy of the genetic code, and (c) complete complements of (a) and (b), wherein the stringent conditions are hybridization at 65Â° C. in hybridization buffer (3.5Ã—SSC, 0.02% Ficoll, 0.02% polyvinyl pyrrolidone, 0.02% Bovine Serum Albumin, 25 mM NaH2PO4 (pH 7), 0.5% SDS, 2 mM EDTA); wherein SSC is 0.15M sodium chloride/0.015M sodium citrate. pH 7: SDS is sodium dodecyl sulphate; and EDTA is ethylenediaminetetracetic acid.