Patent ID: 7989001
Filing Date: 2011-08-02
Classification: A61P,C12N

Abstract:
1. A method of separating tumor cells with lymphotropic metastatic potential from tumor cells without lymphotropic metastatic potential in a human carcinoma comprising the steps of a) transplanting cells of the carcinoma at a transplant site in each of a plurality of T-lymphocyte deficient environments which are adapted to suppress tumor growth of NK-sensitive cells; b) waiting a period of time after the cells are transplanted to determine whether a palpable tumor develops at the transplant site of at least one of the environments; c) alter waiting the period of time, treating with antibodies to NK cells at least one of the environments which does not develop a palpable tumor at the transplant site to overcome tumor growth suppression of NK-sensitive tumor cells in the treated environment; and d) harvesting tumor-forming cells at the transplant site of the treated environment to obtain a cell line of cells with lymphotropic metastatic potential.