Patent ID: 7822559
Filing Date: 2010-10-26
Classification: G16B

Abstract:
1. A method of removing trends in signal intensity values from features on a chemical array, said method comprising: inputting signal intensity values to a system configured for removing trends in signal intensity values from features on a chemical array, from all features on the array except signal intensity values from features removed by filtering to remove at least features having the lowest signal intensity values and control features; calculating, using a processor of said system, a log transform of each signal intensity value inputted to provide log signal intensity values; calculating, using a processor of said system, a surface approximation of the log signal intensity values; normalizing surface fit values on the calculated surface approximation in locations corresponding to locations of the features on the array from which signal intensity values were inputted; calculating a reverse log transform of the normalized surface fit values; de-trending the inputted signal intensity values as a function of the normalized surface values in locations corresponding to the locations of the features from which the signal intensity values were inputted; and outputting de-trended signal intensity values.