Patent ID: 8685720
Filing Date: 2014-04-01
Classification: C12N

Abstract:
1. A composition comprising one or more mammalian vectors, wherein the one or more vectors comprise: a) a first nucleotide sequence comprising a promoter operably linked to a nucleic acid sequence encoding a first cell-cell communication molecule, b) a second nucleotide sequence comprising a promoter operably linked to a nucleic acid sequence encoding a second cell-cell communication molecule that binds with said first cell-cell communication molecule to produce a first binding molecule, c) a third nucleotide sequence comprising a promoter operably linked to a nucleic acid sequence encoding a first cell fate regulator protein that induces differentiation of a first cell type into a second cell type that expresses a marker protein of said second cell type, d) a fourth nucleotide sequence comprising a promoter operably linked to a nucleic acid sequence encoding a first repressor, e) a fifth nucleotide sequence comprising a promoter operably linked to a nucleic acid sequence encoding a first activator, wherein f) a sixth nucleotide sequence comprising a promoter operably linked to a nucleic acid sequence encoding a second repressor, g) a seventh nucleotide sequence comprising a promoter operably linked to a nucleic acid sequence encoding a third repressor, h) a eight nucleotide sequence comprising a promoter operably linked to a nucleic acid sequence encoding a second activator, i) a ninth nucleotide sequence comprising a promoter operably linked to a nucleic acid sequence encoding a fourth repressor, wherein j) a tenth nucleotide sequence comprising a promoter operably linked to a nucleic acid sequence encoding a fifth repressor, k) an eleventh nucleotide sequence comprising a promoter operably linked to a nucleic acid sequence encoding a sixth repressor, wherein 1) a twelfth nucleotide sequence comprising a promoter operably linked to a nucleic acid sequence encoding a seventh repressor wherein m) a thirteenth nucleotide sequence comprising a promoter operably linked to a nucleic acid sequence encoding a second cell fate regulator protein that is operably linked to a cell type specific promoter of said second cell type, wherein the second regulator induces differentiation of said second cell type into a third cell type, wherein expression of said first cell fate regulator protein causes expression of said second cell fate regulator protein and differentiation of said second cell type into said third cell type wherein said fifth repressor represses expression of said twelfth nucleic acid sequence, and wherein said first cell-cell communication molecule and said second cell-cell communication molecule are selected from the group consisting of,