Patent ID: 6620599
Filing Date: 2003-09-16
Classification: C12N,C12P

Abstract:
A non-chromatographic process for the production of an isolated, biologically active phenolic compound (+)-catechin of formula from Taxus wallichiana tissue cultures, said process comprising the steps of:(a) inoculation of Taxus wallichiana explants on a culture medium supplemented with combinations of auxins and cytokinins to prepare the tissue cultures; (b) incubation of the tissue cultures under continuous light or dark conditions for 4-6 weeks for callus initiation followed by subculturing at 4-6 week intervals; (c) extraction of resultant fresh pulverized calli with at least one polar solvent at room temperature; (d) evaporating the at least one solvent to obtain a residuel; and (e) treatment of the residue with a chlorinated solvent and isolation of the (+)-catechin compound by filtration.