Patent ID: 7300755
Filing Date: 2007-11-27
Classification: C12Q

Abstract:
1. A method for separating large fragments of genomic DNA by haplotype, comprising: (a) providing a flat solid surface comprising covalently bound oligonucleotide probes having a nucleotide sequence complementary to each haplotype of an HLA allele; (b) contacting the oligonucleotide probes with a sample of genomic DNA under conditions conducive to specific hybridization of the genomic DNA to the oligonucleotide probes to form a genomic DNA/oligonucleotide complex; (c) removing excess genomic DNA; (d) denaturing the genomic DNA/oligonucleotide complex; and (e) separately collecting the genomic DNA corresponding to each haplotype; thereby separating the large fragments of genomic DNA by haplotype.