Patent ID: 6218138
Filing Date: 2001-04-17
Classification: C12P

Abstract:
A process of synthesizing an amino-group-containing beta-lactam antibiotic, comprising:reacting an amino-beta-lactam component and a corresponding amino-group-containing acylating side-chain component in the presence of a biocatalyst comprising penicillin amidase from E. coli covalently immobilized on support particles to form a reaction product mixture comprising a .beta.-lactam antibiotic product; solubilizing the beta-lactam antibiotic product by adding an acid to the beta-lactam antibiotic product to lower the pH to 1.0 at a temperature in the range of 0.degree. C. to +5.degree. C., said acid substantially inactivating said immobilized penicillin amidase;separating the beta-lactam antibiotic product from the immobilized penicillin amidase and then substantially reactivating said immobilized penicillin amidase by treatment with a buffer having about a neutral pH to provide said immobilized penicillin amidase with an activity suitable for reuse in said process for synthesizing an amino-group-containing beta-lactam antibiotic.