Patent ID: 8673608
Filing Date: 2014-03-18
Classification: A21D,A23C,A23L,A23V,A61K,A61P,A61Q,C08B,C12N,C12P,C12R,Y02P

Abstract:
1. A purified α-glucosyltransferase, which forms a saccharide having α-1,4 linkages which is branched when the said α-glucosyltransferase acts on maltose and α-1,4 glucan having a glucose polymerization degree of 3 or higher; wherein said branched α-1,4 glucan saccharide is composed of glucose molecules linked to each other with α-glucosidic linkages and having the following characteristics upon methylation analysis: (1) Ratio of 2,3,6-trimethyl-1,4,5-triacetyl-glucitol to 2,3,4-trimethyl-1,5,6-triacetyl-glucitol, which indicates the ratio of glucose residues with α-1,4 linkages to α-1,6 linkages, is in the range of 1:0.6 to 1:4; (2) Total content of 2,3,6-trimethyl-1,4,5-triacetyl-glucitol and 2,3,4-trimethyl-1,5,6-triacetyl-glucitol, which indicates that the total content of glucose residues with α-1,4 linkages and glucose residues with 1,6 linkages, is 60% or higher in the partially methylated glucitol acetates; (3) Content of 2,4,6-trimethyl-1,3,5-triacetyl-glucitol, which indicates that the content of glucose residues with α-1,3 linkages, is 0.5% or higher but less than 10% in the partially methylated glucitol acetates; and (4) Content of 2,4-dimethyl-1,3,5,6-tetraacetyl-glucitol, which indicates that the content of glucose residues with α-1,3 linkages and α-1,6 linkages, is 0.5% or higher in the partially methylated glucitol acetates; and wherein said α-glucosyltransferase has the following physicochemical properties: (a) Molecular weight 90,000±10,000 daltons on SDS-polyacrylamide gel electrophoresis; (b) Optimum temperature 50 to 55° C. when reacted at pH 6.0 for 30 min; (c) Optimum pH pH 5.0 to 6.3 when reacted at 40° C. for 30 min; (d) Thermal stability Stable up to the temperature of 40° C. when incubated at pH 6.0 for 60 min; and (e) pH Stability Stable at least in the range of pH 4.0 to 8.0 when incubated at 4° C. for 24 hours.