Patent ID: 6417338
Filing Date: 2002-07-09
Classification: A61K,B82Y,C07K

Abstract:
A method of purifying an isolated autotaxin polypeptide comprising the steps of:i) collecting and concentrating supernatant from cultured cells whereby a first preparation of said polypeptide is produced; ii) salt fractionating said first preparation to produce a second polypeptide preparation; and isolating said polypeptide from said second preparation so that said polypeptide is obtained in substantially pure form, wherein the polypeptide comprises an amino acid sequence of human autotaxin having phosphodiesterase activity and cell motility-stimulating activity, wherein the polypeptide comprises the amino acid sequence N-Tyr-Met-Arg-Pro-Val-Tyr-Pro-Thr-Lys-Thr-Phe-Pro-Asn-C, residues 201 through 213 of SEQ ID NO: 69.