Patent ID: 6936414
Filing Date: 2005-08-30
Classification: C12N

Abstract:
1. A method for separating nucleic acid from a test sample comprising: a) contacting a test sample with a metal oxide support material and a binding buffer such that the nucleic acid bonds with the metal oxide support material to form complexes without prior purification or precipitation of the nucleic acid, wherein the binding buffer comprises and wherein the binding buffer contains no or a low concentration of organic solvent such that the flashpoint of the binding buffer is greater than 130 degrees Fahrenheit; b) separating the complexes from the test sample; and c) eluting the nucleic acid from the metal oxide support material, thereby separating the nucleic acid from the test sample, wherein step a) allows the nucleic acids to be directly employed in an amplification reaction without exchanging an elution buffer and wherein the test sample is selected from the group consisting of blood, ocular lens fluid, cerebral fluid, milk, ascites fluid, synovial fluid, peritoneal fluid, amniotic fluid, tissue, fermentation broth, and cell culture.