Patent ID: 7241587
Filing Date: 2007-07-10
Classification: C12N,C12P

Abstract:
1. A process for producing a product in a recombinant bacterial host cell comprising, a) uncoupling an oxidative membrane bound productive pathway, wherein glucose is oxidized to gluconate from a catabolic metabolic pathway selected from the group of glycolyals, pentose pathway or both in a bacterial host cell, comprising inactivating by homologous recombination a polynucleotide encoding a glucokinase having at least 95% sequence identity to SEQ ID NO: 2, which phosphoryletes glucose as an initial carbon substrate an the catabolic pathway, b) culturing the bacterial host cell under conditions suitable for the production of a product in the presence of a carbon source comprising glucose, and c) obtaining the product, wherein the product is gluconic acid, 2-keto-D-gluconate (2-KDG), 2,5-diketo-D-gluconate (2,5-DKG), 2-keto-L-gulonic acid (2-KLG), erythorbic acid, 5-keto-D-gluconate (5-KDG), tartaric acid, or idonic acid.