Patent ID: 8197852
Filing Date: 2012-06-12
Classification: A61K,A61P,C07K

Abstract:
1. A process for obtaining solubilized proteins from eggshell membrane without the use of proteolytic enzymes or cross-linking agents, said process comprising: (a) exposing the eggshell membrane to a sufficient amount of a basic solution for a sufficient length of time and temperature for hydrolysis of the eggshell membrane to occur so as to produce a solution containing solubilized proteins whereby the solubilized proteins are undenatured and retain biological activity; (b) cooling the solubilized protein solution to a temperature of from about 2° C. to about 18° C.; (c) centrifuging the cooled solubilized protein solution to form a supernatant liquid; (d) removing ash from the supernatant liquid so that the supernatant liquid has a conductivity of less than 5 milliSiemens/cm; and (e) recovering a solubilized protein composition from the supernatant liquid obtained in step (d) by filtering the supernatant liquid through a membrane that has a molecular cutoff of at least 100 kDa to obtain a retentate protein mixture containing solubilized proteins having a molecular weight of greater than 100 kDa and/or a permeate protein mixture containing solubilized proteins having a molecular weight of less than 100 kDa.