Patent ID: 7211433
Filing Date: 2007-05-01
Classification: C12N

Abstract:
1. A method for simultaneously enriching tumor cells and depleting unwanted blood cells from a body fluid, comprising (a) centrifuging in a centrifugation vessel a cell separation medium overlaid with said body fluid, wherein said cell separation medium has a density in the range of from 1.055 to 1.065 g/ml, and wherein said centrifugation vessel is divided into an upper compartment and a lower compartment, and (b) introducing said cell separation medium into said lower compartment and said body fluid into said upper compartment, wherein said upper and lower compartments are divided by a porous barrier, filter, sieve, or flap, wherein said flap is a disk, wherein said disk is bent by centrifugation on two sides across a transverse strut.