Patent ID: 6835550
Filing Date: 2004-12-28
Classification: A61P,C12N,G01N

Abstract:
A method for determining a T-cell epitope of a peptide, comprising the steps of:(a) obtaining from a single human blood source a solution of dendritic cells and a solution of naÃ¯ve CD4+ and/or CD8+ T-cells; (b) differentiating said dendritic cells by exposing said dendritic cells to granulocyte-macrophage colony stimulating in factor (GM-CSF,) interleukin-4 (IL-4), tumor necrosis factor alpha (TNF-&agr;), and interleukin-1 alpha (IL-1&agr;); (c) combining said solution of differentiated dendritic cells and said naÃ¯ve CD4+ and/or CD8+ T-cells with the peptide, said peptide comprising said T-cell epitope; and (d) measuring proliferation of said T-cells in said step (c).