Patent ID: 6228626
Filing Date: 2001-05-08
Classification: C12N,C12Y

Abstract:
An isolated mutant sarcosine oxidase derived from randomly mutating the DNA sequence encoding the wild-type sarcosine oxidase of Bacillus sp. NS-129 of SEQ ID NO: 1, wherein the mutant sarcosine oxidase has the following physico-chemical properties:(a) action: oxidatively hydrolyzing 1 mole sarcosine to give 1 mole glycine, 1 mole formaldehyde, and 1 mol hydrogen peroxide;(b) substrate specificity: specific for sarcosine;(c) optimum pH: 7.0-8.0;(d) stable pH range: 7.0-9.5;(e) suitable temperature for action: 50.degree. C.; and(f) thermostability: 55.degree. C. or less for at least 10 minutes.