Patent ID: 6472207
Filing Date: 2002-10-29
Classification: C12N,C12Q

Abstract:
A eucaryotic cell containing at least one gene tagged in accordance with the method comprising(1) producing a tagged gene by inserting a DNA sequence into an intron of a gene by:(a) selecting a DNA sequence: (i) having a 5â€² portion free of any nucleotide sequence selected from the group consisting of CAGGTAAGT, CAGGTGAGT, AAGGTAAGT, AAGGTGAGT, followed by; (ii) a nucleotide sequence selected from the group consisting of TACTAAC, TGCTAAC, TCCTAAC, TCCTAAC, TACTGAC, TGCTGAC, TCCTGAC, TTCTGAC and TACTAAC and any nucleotide sequence identical to a known splice branch site in a known gene, followed by; (iii) a sequence selected from the group consisting of a sequence 14 to 34 nucleotides in length and any nucleotide sequence identical in length to a known spacer region between splice branch and acceptor sites in a known gene, followed by; (iv) a nucleotide sequence selected from the group consisting of CAGG, TAGG, and any sequence identical to a known splice acceptor site in a known gene, followed by; (v) an open reading frame encoding a known peptide tag recognizable by a known reaction characteristic of said known peptide tag, followed by; (vi) a nucleotide sequence selected from the group consisting of CAGGTAAGT, CAGGTGAGT, AAGGTAAGT, AAGGTGAG and any sequence identical to a known splice donor site in a known gene; and (b) inserting said DNA sequence into said intron within said gene to create a tagged gene; and (2) incubating said tagged gene within a eucaryotic cell so as to maintain intact or to introduce said tagged gene within the genome of said eucaryotic cell.