Patent ID: 7655442
Filing Date: 2010-02-02
Classification: C07D,C12N,C12P

Abstract:
1. A method for producing a macrolide compound selected from the group consisting of (8E,12E,14E)-3,6,7,21-tetrahydroxy-6,10,12,16,20-pentamethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-7-acetoxy-3,6,21-trihydroxy-6,10,12,16,20-pentamethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-7-acetoxy-3,6-dihydroxy-6,10,12,16,20-pentamethyl-21-oxo-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-7-acetoxy-3,6,16,21-tetrahydroxy-6,10,12,16,20-pentamethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-7-acetoxy-3,6,20,21-tetrahydroxy-6,10,12,16,20-pentamethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-3,6,7,16,21-pentahydroxy-6,10,12,16,20-pentamethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-3,6,7,20,21-pentahydroxy-6,10,12,16,20-pentamethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (4E,8E,12E,14E)-7-acetoxy-3,6,21-trihydroxy-6,10,12,16,20-pentamethyl-18,19-epoxytricosa-4,8,12,14-tetraen-11-olide; (8E,12E,14E)-3,6,21-trihydroxy-6,10,12,16,20-pentamethyl-7-propanoyloxy-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-7-acetoxy-3,6,21-trihydroxy-6,10,12,16,20-pentamethyl-18,19-epoxydocosa-8,12,14-trien-11-olide; (8E,12E,14E)-7-acetoxy-3,5,6,21-tetrahydroxy-6,10,12,16,20-pentamethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-5,7-diacetoxy-3,6,21-trihydroxy-6,10,12,16,20-pentamethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-3,7-diacetoxy-6,21-dihydroxy-6,10,12,16,20-pentamethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-7-acetoxy-6-acetoxymethyl-3,6,21-trihydroxy-10,12,16,20-tetramethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-7-acetoxy-3,6,17,21-tetrahydroxy-6,10,12,16,20-pentamethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-7-acetoxy-3,6,20-trihydroxy-6,10,12,16-tetramethyl-18,19-epoxyheneicosa-8,12,14-trien-11-olide; (4E,8E,12E,14E)-3,6,7,21-tetrahydroxy-6,10,12,16,20-pentamethyl-18,19-epoxytricosa-4,8,12,14-tetraen-11-olide; (8E,12E,14E)-7-acetoxy-3,6,21-trihydroxy-6,10,12,16-tetramethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-7-acetoxy-3,6,21-trihydroxy-6,10,12,20-tetramethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (12E,14E)-3,6,21-trihydroxy-6,10,12,16,20-pentamethyl-9-oxo-18,19-epoxytricosa-12,14-dien-11-olide; (8E,12E,14E)-7-acetoxy-3,6,21-trihydroxy-6,10,16,20-tetramethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-7-acetoxy-3,6,21-trihydroxy-2,6,10,12,16,20-hexamethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-7-acetoxy-3,5,21-trihydroxy-6,10,12,16,20-pentamethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-7-acetoxy-3,6,21-trihydroxy-6,12,16,20-tetramethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-6-acetoxymethyl-3,6,7,2,1-tetrahydroxy-10,12,16,20-tetramethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-3,6,7-trihydroxy-6,10,12,16,20-pentamethyl-21-oxo-18,19-epoxytricosa-8,12,14-trien-11-olide; a 3-position isomer of (8E,12E,14E)-7-acetoxy-3,6,21-trihydroxy-6,10,12,16,20-pentamethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-7-acetoxy-3,6,21-trihydroxy-10,12,16,20-tetramethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-6-acetoxy-3,7,21-trihydroxy-10,12,16,20-tetramethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-3,6,7,21-tetrahydroxy-2,6,10,12,16,20-hexamethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E,18E)-7-acetoxy-3,6,21,22-tetrahydroxy-6,10,12,16,20-pentamethyltricosa-8,12,14,18-tetraen-11-olide; (8E,12E,14E)-3,7,21-trihydroxy-10,12,16,20-tetramethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (4E,8E,12E,14E)-7-acetoxy-3,6-dihydroxy-6,10,12,16,20-pentamethyl-21-oxo-18,19-epoxytricosa-4,8,12,14-tetraen-11-olide; (8E,12E,14E)-7-acetoxy-3,21-dihydroxy-10,12,16,20-tetramethyl-18,19-epoxy-6,6-(epoxymethano)tricosa-8,12,14-trien-11-olide; (4E,8E,12E,14E)-7-acetoxy-3,21-dihydroxy-10,12,16,20-tetramethyl-18,19-epoxy-6,6-(epoxymethano)tricosa-4,8,12,14-tetraen-11-olide; (8E,12E,14E)-3,7,21-trihydroxy-10,12,16,20-tetramethyl-18,19-epoxy-6,6-(epoxymethano)tricosa-8,12,14-trien-11-olide; (4E,8E,12E,14E)-6,7-diacetoxy-3,21-dihydroxy-6,10,12,16,20-pentamethyl-18,19-epoxytricosa-4,8,12,14-tetraen-11-olide; (8E,12E,14E)-6,7-diacetoxy-3,21-dihydroxy-6,10,12,16,20-pentamethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-7-acetoxy-3,6,16-trihydroxy-6,10,12,16,20-pentamethyl-21-oxo-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-7-acetoxy-3,6,21,22-tetrahydroxy-6,10,12,16,20-pentamethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (4E,8E,12E,14E)-7-acetoxy-3,6,17,21-tetrahydroxy-6,10,12,16,20-pentamethyl-18,19-epoxytricosa-4,8,12,14-tetraen-11-olide; (8E,12E,14E)-7-acetoxy-3,6,17-trihydroxy-6,10,12,16-tetramethyl-18,19-epoxyheneicosa-8,12,14-trien-11-olide; (8E,12E,14E)-7-acetoxy-3,5,6,21,22-pentahydroxy-6,10,12,16,20-pentamethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-7-acetoxy-3,6,16-trihydroxy-6,10,12,16-tetramethyl-18,19-epoxyheneicosa-8,12,14-trien-11-olide; (8E,12E,14E)-3,6,7,21-tetrahydroxy-6,10,16,20-tetramethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-7-acetoxy-3,6,17,21-tetrahydroxy-6,10,12,16,20-pentamethyl-18,19-epoxytricosa-8,12,14-trien-11-olide; (8E,12E,14E)-7-acetoxy-3,6,17-trihydroxy-6,10,12,16,18-pentamethyl-18,19-epoxyheneicosa-8,12,14-trien-11-olide; (8E,12E,14E)-7-acetoxy-3,6,21-trihydroxy-6,10,12,16,20-pentamethyl-5-oxo-18,19-epoxytricosa-8,12,14-trien-11-olide; and (8E,12E,14E,18E)-7-acetoxy-3,6,21-trihydroxy-6,10,12,16,20-pentamethyltricosa-8,12,14,18-tetraen-11-olide, said method comprising the steps of: providing cyclodextrin in a culture broth of actinomycetes having an ability of producing the macrolide compound, culturing said actinomycetes in said culture broth to produce the macrolide compound, and separating the macrolide compound from said culture broth.