Patent ID: 6395493
Filing Date: 2002-05-28
Classification: B01J,C12Q,H01L

Abstract:
A method for deterring the nature of repeat units in a genetic target, comprising the steps of:providing in the presence of an applied electric field a plurality of hybridization complex assays arrayed on a plurality of test sites by: providing a nucleic acid target containing repetitive DNA sequences, providing a capture probe having a first unique flanking sequence and n repeat units, where n&gE;0, complementary to the target sequence, and providing a reporter probe having a selected sequence complementary to the same target sequence strand, the reporter including attributes selected from the group consisting of: a) a second unique flanking sequence, b) a sequence complementary to a variant region of the target sequence, and c) a second unique flanking sequence and a sequence complementary to a variant region of the target sequence, wherein the sequence complementary to a variant region may comprise one or more repeat units, wherein the sum of the number of repeat units in the capture plus the reporter is greater than zero, the sequence of the capture probe differing at at least two test sites of the array, determining concordance and discordance among the hybridization complex assays at the test sites as determined at least in part by hybridization stability and determining the nature of the repeat units in the target sequences based upon determination of the concordant site and further based upon the knowledge of the pre-determined sequences of the probes located in the hybridization complex at that site.