Patent ID: 6733964
Filing Date: 2004-05-11
Classification: B01J,B82Y,C12Q,C40B,G01N,G16B

Abstract:
A computer implemented method of identifying an unknown base in a sample nucleic acid sequence, said method comprising the steps of:inputting a plurality of probe intensities for an array of nucleic acid probes that differ from each other by a single base at an internal interrogation position into a computer system, each probe intensity indicating an extent of hybridization of a nucleic acid probe with at least one nucleic acid sequence including said sample sequence; said computer system comparing said plurality of probe intensities to each other, wherein said comparing step includes the step of said computer system calculating a ratio of a higher probe intensity to a lower probe intensity; and said computer system generating a base call identifying said unknown base according to results of said comparing step and said sequences of said nucleic acid probes.