Patent ID: 6472204
Filing Date: 2002-10-29
Classification: A61K,C07K,C12N

Abstract:
An ex vivo method for increasing the efficiency of retroviral mediated gene transfer into viable target cells, which comprises transducing the target cells by infecting said target cells with a replication defective recombinant retrovirus that infects said target cells in an aqueous medium in the presence of(a) a mixture of an effective amount of a first functional material having a retrovirus binding domain that binds said retrovirus and an effective amount of a second functional material having a target cell binding domain that binds said target cell, or (b) an effective amount of a bifunctional material having both a retroviral binding domain which does not contain the heparin binding domain derived from human fibronectin, and a target cell binding domain, wherein said bifunctional material has a retrovirus binding domain that binds said retrovirus and a target cell binding domain that binds said target cell.