Patent ID: 9144585
Filing Date: 2015-09-29
Classification: A61K,A61L,C12N

Abstract:
1. A method of isolating an extracellular matrix, comprising: (a) obtaining induced pluripotent stem cells (iPSCs) derived from plucked human hair follicle keratinocytes (HFKTs); (b) generating embryoid bodies (EBs) from said iPSCs; (c) generating single cells from adherent cells of said EBs, and (d) culturing said single cells in a culture medium which comprises ascorbic acid and does not comprise dexamethasone to thereby obtain a population of mesenchymal progenitor cells which comprises at least 70% CD105+ cells, wherein said mesenchymal progenitor cells exhibit at least 70% reduced differentiation potential into an adipogenic lineage as compared to differentiation of mesenchymal stem cells from an adult adipose source under identical assay conditions as analyzed after 28-30 days of culturing in a medium which comprises 10 (e) culturing said mesenchymal progenitor cells produced in step (d) under conditions which induce production of extracellular matrix from said mesenchymal progenitor cells, and subsequently, (f) isolating the extracellular matrix produced by said mesenchymal progenitor cells, thereby isolating the extracellular matrix.