Patent ID: 8626337
Filing Date: 2014-01-07
Classification: B07C,G01N

Abstract:
1. A method for distinguishing seeds containing a genetic element of interest from a bulk sample, the method comprising: associating a red fluorescent protein marker with at least some of the seeds of the bulk sample; evaluating at least some of the seeds of the bulk sample for the presence or absence of the red fluorescent protein marker using a seed color sorter; and sorting the seeds containing a genetic element of interest based on the presence or absence of the red fluorescent protein marker; wherein the sorting step comprises dispensing the sorted individual seeds into at least one container, the container including seeds having the red fluorescent protein marker present or seeds in which the red fluorescent protein marker is absent, and wherein individual seeds are singulated from the bulk sample using a singulating device prior to said evaluating at least some of the seeds of the bulk sample for the presence or absence of the red fluorescent protein marker, and further comprising associating a plurality of supplemental markers with at least some of the seeds containing a corresponding plurality of additional genetic elements of interest of the bulk sample; evaluating at least one of the seeds of the bulk sample for the presence or absence of the plurality of supplemental markers using the seed color sorter; and sorting the seeds containing the plurality of additional genetic elements of interest based on the presence or absence of the a plurality of supplemental markers.