Patent ID: 8143017
Filing Date: 2012-03-27
Classification: G01N

Abstract:
1. A method of measuring the total antioxidant status (TAS) of a biological sample obtained from a human or animal organism to determine said organism's ability to withstand reactive oxygen species (ROSs), comprising the following steps: a) putting the biological sample for testing into contact with a photosensitive agent in a liquid medium to form a mixture for testing; b) subjecting said mixture for testing to a dose of light irradiation at a wavelength that is absorbed by the photosensitive agent in order to give rise, by a photochemical reaction at least between the light and the photosensitive agent, to at least the production of singlet oxygen suitable for co-operating with said sample to form ROSs; c) adding, after irradiation, a compound that reacts colorimetrically in the presence of ROSs to form a chromogen or fluorescent substance; d) measuring the quantity of chromogen or fluorescent substance produced over time in order to determine the ability of said sample to withstand ROSs by inhibiting them, a low level of chromogen or fluorescent substance production corresponding to said sample having a high ability to withstand ROSs; e) subjecting at least one reference mixture formed by putting a reference biological sample from a presumed healthy organism in contact with a photosensitive agent to a dose of light irradiation at a wavelength that is absorbed by the photosensitive agent to form ROSs, adding a compound that reacts colorimetrically in the presence of ROSs to form a chromogen or fluorescent substance, and measuring the quantity of chromogen or fluorescent substance produced over time as carried out for the mixture for testing; and f) comparing the measured quantity of chromogen or fluorescent substance obtained from the mixture for testing with the measured quantity of chromogen or fluorescent substance obtained from said at least one reference mixture to obtain the TAS of the biological sample of said organism and determine said organism's ability to withstand ROSs.