Patent ID: 6569619
Filing Date: 2003-05-27
Classification: C12Q,G01N

Abstract:
A method for determining helicase activity, said method comprising:incubating a reaction mixture comprising a first nucleic acid hybridized to a complementary unlabeled second nucleic acid, a helicase polypeptide and a nucleoside triphosphate under conditions suitable for said helicase polypeptide to unhybridize said first nucleic acid and said second nucleic acid; adding an excess of a third nucleic acid comprising an immobilizable tag to said reaction mixture, wherein said third nucleic acid is complementary to said first nucleic acid; incubating said reaction mixture under conditions suitable for said third nucleic acid to hybridize to said first nucleic acid to form an immobilizable hybridization complex, whereby said immobilizable hybridization complex is immobilized on a solid support; and detecting the presence of said immobilizable hybridization complex on said solid support.