Patent ID: 8323573
Filing Date: 2012-12-04
Classification: B01L,G01N,Y10T

Abstract:
1. A microfluidic cartridge with solution reservoir-pump chamber comprising: a channel-chamber layer, a sealing layer for the channel-chamber layer, a printed circuit board bound with magnetoresistive biochip, and at least one sealable solution inlet, characterized in that: the channel-chamber layer is provided with a waste reservoir, a reaction-detection chamber, at least one solution reservoir-pump chamber and at least one micro-channel wherein the waste reservoir has a waste outlet and communicates with the reaction-detection chamber via the micro-channel, and the other port of the reaction-detection chamber communicates with said solution reservoir-pump chamber and said sealable solution inlet via said micro-channel; said solution reservoir-pump chamber includes a sample reservoir and a reagent reservoir with each consists of a cylindrical chamber and an elastic element used for sealing and pumping the liquid in said cylindrical chamber, wherein said elastic element is an elastic spheroid or cylinder; the sealing layer and the channel-chamber layer are aligned and sealed so as to completely form the waste reservoir, the reaction-detection chamber, the sealable solution inlet, the solution reservoir-pump chamber and the micro-channel; a chip window is formed in a position of the sealing layer corresponding to the position of the reaction-detection chamber in said channel-chamber layer, wherein said chip window is aligned and sealed between said reaction-detection chamber and said magnetoresistive biochip on said printed circuit board to form a complete reaction-detection chamber with the surface of said magnetoresistive chip as the reaction and detection surface; and the surface of the magnetoresistive chip is bio-functionalized to form a biochip which is connected to a testing apparatus outside said microfluidic cartridge by a plurality of electrically conductive lines on the printed circuit board.