Patent ID: 8759030
Filing Date: 2014-06-24
Classification: C12P

Abstract:
1. A process for producing 2-O-α-glucopyranosyl-L-ascorbic acid, comprising the steps of: allowing α-isomaltosyl glucosaccharide-forming enzyme (IMG) but not cyclomaltodextrin glucanotransferase (EC 2.4.1.19) (CGTase) to act on a solution comprising L-ascorbic acid and liquefied corn starch to obtain a reaction mixture containing 2-O-α-glucopyranosyl-L-ascorbic acid in an amount of 10% (w/w) or higher and each of 5-O-α-glucopyranosyl-L-ascorbic acid and 6-O-α-glucopyranosyl-L-ascorbic acid in an amount of less than 0.1% (w/w), on a dry solid basis; and collecting the 2-O-α-glucopyranosyl-L-ascorbic acid from the reaction mixture; wherein said α-isomaltosyl glucosaccharide-forming enzyme has an activity of forming a saccharide with a glucose polymerization degree of 3 or higher and bearing both the α-1,6 glucosidic linkage as a linkage at the non-reducing end and the α-1,4 glucosidic linkage other than the linkage at the non-reducing end from a saccharide with a glucose polymerization degree of 2 or higher and bearing the α-1,4 glucosidic linkage as a linkage at the non-reducing end by α-glucosyl-transferring reaction without increasing the reducing power of the reaction mixture; wherein said α-isomaltosyl glucosaccharide-forming enzyme is obtained from