Patent ID: 6103519
Filing Date: 2000-08-15
Classification: A61K,A61P,C07K,C12N

Abstract:
A recombinant DNA molecule comprising a DNA coding sequence for expression of a modified L protein, which is operatively linked to a regulatory sequence, wherein said modified L protein is lacking amino acids 1-11 of naturally occurring L protein, containing at least amino acids 21-47 of the naturally occurring pre-S1 region, and containing at least amino acids 133-145 of the pre-S2 region,wherein said modified hepatitis B virus large surface protein is further characterized by at least one modification selected from the group consisting of:at least one of the protease sensitive sites at Arg100, Arg137, Gly149, or Arg167 is modified or deleted;a myristylation site at amino acid residue 13 is lacking;at least one of the glycosylation sites at amino acid residue 123, 124, or 125 is modified or deleted;and a human serum albumin binding site at amino acids 120-132 of the naturally occurring pre-S2 region is lacking.