Patent ID: 7378236
Filing Date: 2008-05-27
Classification: C12Q

Abstract:
1. A method of constructing a subarray of distinct gene sequences whose expression levels are specifically related to differences between test cells relative to control cells, comprising: (a) obtaining and preparing reporter-labeled copies of messenger nucleic acid from said control cells in a population of control individuals, and from said test cells in a population of test individuals having a shared phenotype that is not present in control individuals, wherein the reporter is compatible with a fluorescence detection system, (b) applying the reporter-labeled nucleic acids from test and control cells to a microarray of distinct gene sequences, under conditions effective to hybridize the reporter-labeled nucleic acids to complementary genes sequences on the microarray, wherein the microarray comprises at least 1000 distinct gene sequences per cm (c) comparing the pattern of reporter levels for nucleic acids from the test cells with that of nucleic acids from the control cells, (d) identifying those test-cell distinct gene sequences on the microarray which show a significant elevation or reduction in reporter levels, when compared with control reporter levels, and (e) forming a subarray of said test-cell distinct gene sequences.