Patent ID: 6338964
Filing Date: 2002-01-15
Classification: C12N

Abstract:
A cell culture medium, comprising:an organic buffer selected from the group consisting of 3-(N-morpholino)propanesulfonic acid, N,N-bis (2-hydroxyethyl)-2-aminoethanesulfonic acid, N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid, and tris(hydroxymethyl)aminoethane, wherein the buffer concentration is greater than 2 millimolar, a metal complexing agent selected from the group consisting of histidine, iminodiacetic acid, citrate, phosphoserine, aurintricarboxylate and, lysine hydroxamate, wherein the complexing agent concentration is greater than 5 millimolar, and less than approximately 1 gram/L of added sodium bicarbonate.