Patent ID: 6010886
Filing Date: 2000-01-04
Classification: C07K,G01N

Abstract:
An isolated DNA encoding a recombinant fusion protein of the formula:R.sub.1 --L--R.sub.2 or R.sub.2 --L--R.sub.1.wherein R.sub.1 represents a polypeptide selected from the group consisting of:(a) a polypeptide comprising amino acids -22 to y or 1 to y of SEQ ID NO:2, wherein y represents an integer between 308 and 597, inclusive;(b) a polypeptide comprising amino acids encoded by nucleotides 244 to y or 310 to y of SEQ ID NO:1, wherein y represents an integer between 1231 and 2098, inclusive; and(c) a polypeptide comprising an amino acid sequence encoded by the complement of a polynucleotide that hybridizes under highly stringent conditions to a polynucleotide which encodes the polypeptide of (b), wherein highly stringent conditions include hybridization at 68.degree. C. followed by washing in 0.1XSSC/0.1%SDS at 63.degree. C.:and, wherein R.sub.2 represents a polypeptide selected from the group consisting of:(a') a polypeptide comprising amino acids -27 to x or 1 to x of SEQ ID NO:6, wherein x is an integer between 432 and 714, inclusive;(b') a polypeptide comprising amino acids encoded by nucleotides 368 to x or 448 to x wherein x is an integer between 1742 and 2588 of SEQ ID NO:5, inclusive; and(c') a polypeptide comprising an amino acid sequence encoded by the complement of a polynucleotide that hybridizes under highly stringent conditions to polynucleotide which encodes the polypeptide of (b'), wherein highly stringent conditions include hybridization at 68.degree. C. followed by washing in 0.1XSSC/0.1%SDS at 63.degree. C.;and L represents a peptide linker, and wherein the recombinant fusion is capable of enhanced binding to oncostatin M when compared to gp130 binding to oncostatin M.