Patent ID: 7759106
Filing Date: 2010-07-20
Classification: A61K,C12N,C12P,C12Q,Y02A

Abstract:
1. Primer pairs characterized as having a length of about 15-100 nucleotides, which pairs permit the PCR amplification of the entire CfaA, CfaB and CfaE open reading frames without the entire CFA/I operon but with the signal sequence of each protein along with restriction sites for insertion into a vector which insertion allows for expression, export and assembly of the protein on the bacterial surface, which pairs contain sequences or sequences strictly complementary thereto and are selected from the group consisting of: a) 5′-GATCAAGCTTCCATGAAAAAGGAGGGATGTA-3′ SEQ ID NO 1 and 5′-GATCCCATGGGCATGCATAAATTATTCTATTTACTAAGT-3′ SEQ ID NO 2, b) 5′-TACATGCCATGGATAAATTATTCTATTTACT-3′ SEQ ID NO 3 and 5′-ATTCTGTTATATATGTCAACCTGCAGGAGGGATGTATAAACATACC-'3 SEQ ID NO 4, c) 5′-GGTATGTTTATACATCCCTCCTGCAGGTTGACATATATAACAGAAT-3′ SEQ ID NO 5 and 5′-TTACCCAAGCTTAGACATGCTTTTAAAGCAAA-3′ SEQ ID NO 6 and d) 5′-TACATGCCATGGATAAATTATTCTATTTACT-3′ SEQ ID NO 7 and 5′-CGTTTATCCTTTATCATTCTCTTAGTATATAGATGAGTAA-3′ SEQ ID NO 8, e) 5′-TTACTCATCTATATACTAAGAGAATGATAAAGGATAAACG-3′ SEQ ID NO 9 and 5′-TTCAGCCCAAGCTTTAGCGCCAATATGTTGTTAT-3′ SEQ ID NO 10.