Patent ID: 6017762
Filing Date: 2000-01-25
Classification: C12N

Abstract:
An endothiapepsin-producing Cryphonectria parasitica strain, wherein said strain is transformed with a cassette for the expression of an endothiapepsin precursor of Cryphonectria parasitica, said cassette comprising a functional promoter upstream of a nucleic acid sequence coding for said precursor, wherein endothiapepsin has the following amino acid sequence (PI) (SEQ ID NO:1), wherein said precursor is preproendothiapepsin comprising the following amino acid sequence (P4) (SEQ ID NO:2):Met Ser Ser Pro Leu Lys Asn Ala Leu Val Thr Ala Met Leu Ala Gly - Gly Ala Leu Ser Ser Pro Thr Lys Gln His Val Gly Ile Pro Val Asn - Ala Ser Pro Glu Val Gly Pro Gly Lys Tyr Ser Phe Lys Gln Val Arg - Asn Pro Asn Tyr Lys Phe Asn Gly Pro Leu Ser Val Lys Lys Thr Tyr - Leu Lys Tyr Gly Val Pro Ile Pro Ala Trp Leu Glu Asp Ala Val Gln - Asn Ser Thr Ser Gly Ler Ala Glu Arg Ser Thr Gly Ser Ala Thr Thr - Thr Pro Ile Asp Ser Leu Asp Asp Ala Tyr Ile Thr Pro Val Gln Ile - Gly Thr Pro Ala Gln Thr Leu Asn Leu Asp Phe Asp Thr Gly Ser Ser - Asp Leu Trp Val Phe Ser Ser Glu Thr Thr Ala Ser Glu Val Asp Gly - Gln Thr Ile Tyr Thr Pro Ser Lys Ser Thr Thr Ala Lys Leu Leu Ser - Gly Ala Thr Trp Ser Ile Ser Tyr Gly Asp Gly Ser Ser Ser Ser Gly - Asp Val Tyr Thr Asp Thr Val Ser Val Gly Gly Leu Thr Val Thr Gly - Gln Ala Val Glu Ser Ala Lys Lys Val Ser Ser Ser Phe Thr Glu Asp - Ser Thr Ile Asp Gly Leu Leu Gly Leu Ala Phe Ser Thr Leu Asn Thr - Val Ser Pro Thr Gln Gln Lys Thr Phe Phe Asp Asn Ala Lys Ala Ser - Leu Asp Ser Pro Val Phe Thr Ala Asp Leu Gly Tyr His Ala Pro Gly - Thr Tyr Asn Phe Gly Phe Ile Asp Thr Thr Ala Tyr Thr Gly Ser Ile - Thr Tyr Thr Ala Val Ser Thr Lys Gln Gly Phe Trp Glu Trp Thr Ser - Thr Gly Tyr Ala Val Gly Ser Gly Thr Phe Lys Ser Thr Ser Ile Asp - Gly Ile Ala Asp Thr Gly Thr Thr Leu Leu Tyr Leu Pro Ala Thr Val - Val Ser Ala Tyr Trp Ala Gln Val Ser Gly Ala Lys Ser Ser Ser Ser - Val Gly Gly Tyr Val Phe Pro Cys Ser Ala Thr Leu Pro Ser Phe Thr - Phe Gly Val Gly Ser Ala Arg Ile Val Ile Pro Gly Asp Tyr Ile Asp - Phe Gly Pro Ile Ser Thr Gly Ser Ser Ser Cys Phe Gly Gly Ile Gln - Ser Ser Ala Gly Ile Gly Ile Asn Ile Phe Gly Asp Val Ala Leu Lys - Ala Ala Phe Val Val Phe Asn Gly Ala Thr Thr Pro Thr - Leu Gly Phe - Ala Ser Lys