Patent ID: 8158426
Filing Date: 2012-04-17
Classification: C12M,C12N

Abstract:
1. A method of culturing cells comprising: a. forming a cell culture device comprising a single compartment, at least the bottom of said single compartment being comprised at least in part of non porous gas permeable material; b. adding a volume of medium and adding cells into said single compartment, said cells being an animal cell type; c. adjusting the volume of said medium as needed so that said medium resides at a height beyond 2.0 cm from the lowest point of said bottom when said cell culture device resides in a position wherein said bottom comprises the lowest surface of said single compartment; d. placing said cell culture device in a cell culture location that includes ambient gas at a composition for cell culture, wherein said cell culture device is oriented in a position such that said bottom comprises the lowest surface of said single compartment; e. allowing said cells to settle out of said medium by gravity; and f. culturing said cells in a mode of static cell culture after said cells settle out of said medium by gravity.