Patent ID: 7256017
Filing Date: 2007-08-14
Classification: B41M,C07K,C12N,C12R,Y02A,Y10S,Y10T

Abstract:
1. A method of generating a modified Cry1Ca polypeptide having an increased insecticidal activity against a Lepidopteran insect over a native Cry1Ca polypeptide comprising the steps of: (a) identifying in a native Cry1Ca polypeptide a ioop region between α-helices 4 and 5 of domain 1 and a loop region between a-helices 6 and 7 of domain 1; (b) modifying said native Cry1Ca polypeptide by introducing one or more mutations into one or more amino acids within said identified loop regions to produce a modified Cry1Ca polypeptide; and (c) testing said modified Cry1Ca polypeptide to determine whether the modified Cry1Ca polypeptide exhibits increased insecticidal activity against said Lepidopteran insect over said native Cry1Ca polypeptide, thereby generating a modified Cry1Ca polypeptide having an increased insecticidal activity against a Lepidopteran insect over a native Cry1Ca polypeptide.