Patent ID: 6453244
Filing Date: 2002-09-17
Classification: C12Q

Abstract:
A method for analyzing the nucleotide sequence of a nucleic acid in a sample, said method comprising:preconditioning a sample comprising a first homoduplexed nucleic acid molecule and a second homoduplexed nucleic acid molecule, wherein said first and second homoduplexed nucleic acid molecules differ by at least one base pair, said preconditioning providing a completely denatured sample comprising single-stranded nucleic acid molecules, and wherein said preconditioning is by the addition of a compound that increases the pH of the sample; applying the completely denatured sample to a stationary reverse phase support while maintaining complete denaturation of the nucleic acid; and eluting the completely denatured sample under completely denaturing conditions using a mobile phase comprising an ion-pairing reagent and a solvent; wherein said eluting results in the resolution of the single-stranded nucleic acid molecules such that single-stranded nucleic acid molecules that differ in sequence by one nucleotide are separately resolved.