Patent ID: 7005275
Filing Date: 2006-02-28
Classification: A01K,A61K,C07K,C12N

Abstract:
1. A DNA molecule which encodes an RNA molecule comprising: (a) at least one cis-acting sequence element, (b) a first open reading frame which encodes a non-cytopathic temperature-sensitive Sindbis virus replicase, wherein the non-cytopathicity is conferred by a substitution of proline at position 726 of the nonstructural protein 2 (nsP2) of the replicase with another naturally occurring amino acid, and (c) at least one nucleotide sequence selected from the group consisting of: wherein said at least one nucleotide sequence of (c) is operably linked to a promoter which is recognized by said non-cytopathic, temperature-sensitive Sindbis virus replicase.