Patent ID: 8399253
Filing Date: 2013-03-19
Classification: C12N

Abstract:
1. A method for three-dimensionally culturing cells using micro-scaffolds wherein the micro-scaffolds have surface areas allowing for the adhesion and proliferation of the cells, wherein the method is performed in a syringe wherein the syringe has a structure in which the top and inlet of the syringe can be blocked and the piston of the syringe is provided with a passage which can be opened and closed, the method comprising: a) a pretreatment step of centrifuging and washing cells collected in the syringe so as to be suitable for proliferation; b) a micro-scaffold-adhering step of placing medium and micro-scaffolds into the syringe with the collected cells, adhering the cells and micro-scaffolds to each other in a state suspended in the medium; c) a cell culture step of culturing the cells in the syringe; d) a micro-scaffold-gradual addition step of gradually adding micro-scaffolds, in which a small amount of the micro-scaffolds are used in an initial stage and the amount of the micro-scaffolds is then increased according to cell proliferation rate so as to maintain a suitable distance between the cells; e) a periodic shaking step of periodically shaking, in which shaking is performed in order to separate connected cells from each other, after the cells are incubated for more than a given period of time; and wherein one or both of steps d) and e) are performed repeatedly so as to regulate proliferation-inducing and proliferation-inhibitory signal between the cells.