Patent ID: 6737074
Filing Date: 2004-05-18
Classification: A61F,A61K,A61L,A61P,C07K,C12M,C12N

Abstract:
A method for separating cells that bind to fibrin microbeads from a cell culture containing the fibrin microbead binding cells and cells that do not bind to fibrin microbeads, said method comprising the steps of: (i) culturing the cell culture with fibrin microbeads in a culture medium under conditions permitting the fibrin microbead binding cells to bind to the fibrin microbeads, and (ii) isolating the fibrin microbeads bound to the cells from the culture medium, wherein the fibrin microbeads are prepared in the absence of glutaraldehyde as a cross-linking agent, and wherein the fibrin microbeads are prepared by a method comprising the sequential steps of: (i) preparing an aqueous solution comprising fibrinogen, thrombin and Factor XIII; (ii) prior to the onset of coagulation, contacting said aqueous solution with an oil heated to a temperature of about 50-80Â° C. to form an emulsion; (iii) mixing the emulsion at a temperature of about 50-80Â° C. until fibrin microbeads comprising extensively cross-linked fibrin(ogen) are obtained; and (iv) isolating the fibrin microbeads.