Patent ID: 6620988
Filing Date: 2003-09-16
Classification: C07K,Y10S

Abstract:
A method of preparing a Coleopteran-resistant transgenic plant, comprising the steps of:(a) obtaining a nucleic acid segment comprising a gene encoding a modified Cry3Bb* polypeptide, wherein: said polypeptide comprises one or more point mutations in or near &agr; helix 4, wherein said one or more point mutations result in at least one amino acid substitution selected from the group consisting of Leu158 to Arg, Ser160 to Asn, Lys161 to Pro, Pro162 to His, Asp165 to Gly, Lys189to Gly; and wherein said gene is operably linked to a promoter, (b) introducing said nucleic acid segment into a vector, (c) transforming a plant cell with said vector; and (d) regenerating from said plant cell a transgenic plant, which expresses said modified Cry3Bb* polypeptide and wherein said transgenic plant is resistant to Coleopteran insects as compared to a non-transforned plant.