Patent ID: 8669379
Filing Date: 2014-03-11
Classification: C07C,C07D,C12N,C12P

Abstract:
1. An isolated cell that overexpresses a ycdW gene encoding a glyoxylate reductase enzyme, an aceA gene encoding an isocitrate lyase enzyme, and an aceK gene encoding an isocitrate dehydrogenase kinase/phosphatase enzyme, and that recombinantly expresses a pct gene encoding a propionyl-CoA transferase enzyme, at least one of a phaA, thil, atoB or bktB gene encoding a thiolase enzyme, at least one of a phaB or hbd gene encoding a reductase enzyme, and a tesB gene encoding a thioesterase B enzyme, wherein the isolated cell can produce a cell culture that contains at least 0.1 g L −1 2,3-dihydroxybutyrate (2,3-DHBA).