Patent ID: 8110375
Filing Date: 2012-02-07
Classification: B82Y,C12M,C12N

Abstract:
1. A high throughput method of isolating a cancer inhibiting active agent comprising: obtaining at least one slide comprising an upper surface; coating the upper surface of the at least one slide with a base layer, said base layer comprising a first matrix and a plurality of spots, wherein the plurality of spots comprise a plurality of active agents, and wherein each of said plurality of spots has a diameter of about 65 μm to about 3.0 mm; adding a second matrix for mixing with, or for layering atop of, a plurality of cells, and for forming a top layer on top of the base layer, wherein the thickness of at least one of said first matrix and said second matrix is about 0.1 mm to about 1.0 mm; measuring cell growth on each spot; and determining on which spots cell growth is inhibited, wherein the first matrix supports anchorage-independent cell growth and wherein the second matrix limits cell migration and allows three-dimensional cell growth.