Patent ID: 8691506
Filing Date: 2014-04-08
Classification: A61P,C12Q,G16B

Abstract:
1. A diagnostic kit containing a microarray, the microarray comprising the nucleotide sequences of SEQ ID NO.:1 to 118 attached to a substrate, the nucleotide sequences corresponding to the predictive genes of a gene expression profile obtained by a method for providing a gene expression profile being predictive for the specific response of an individual tumor to Avastin, the method comprising the steps of: (a) xenotransplanting human tumor material of at least five different tumors into at least one suitable test animal, (b) determining gene expression profiles of the resulting tumor xenografts, (c) treating said at least one test animal with Avastin, (d) evaluating responsiveness of the tumor xenografts to Avastin, (e) identifying the gene expression profile of each of the tested tumor xenografts, and (f) determining the predictive genes in the gene expression profiles by comparing the gene expression profiles of responsive tumors, no-change tumors and progressive tumors, wherein step (f) comprises the steps of (i) dividing the complete set of gene expression profiles of the tumor xenografts into sub-sets, each of the sub-sets missing one of said gene expression profiles, (ii) determining the predictive genes in each sub-set, and (iii) obtaining the gene expression profile being predictive for the specific response of an individual tumor to Avastin by setting up the intersection of the predictive genes of all sub-sets.