Patent ID: 8431395
Filing Date: 2013-04-30
Classification: A01K,A61K,C12N

Abstract:
1. A method of deriving a pluripotent rat cell from a blastocyst, comprising: (1) culturing a blastocyst in the presence of a MEK1 inhibitor at a concentration ranging from 0.1 μM to 5 μM, a GSK3 inhibitor at a concentration ranging from 0.1 μM to 20 μM, and LIF, to obtain an inner cell mass; (2) isolating and dissociating the primary outgrowths of the inner cell mass; (3) isolating a cell or cells from the dissociated primary outgrowths of the inner cell mass; and (4) culturing the isolated cell or cells in the presence of a MEK1 inhibitor at a concentration ranging from 0.1 μM to 5 μM, a GSK3 inhibitor at a concentration ranging from 0.1 μM to 20 μM, an antagonist of an FGF receptor at a concentration ranging from 0.5 μM to 10 μM, and LIF.