Patent ID: 6488857
Filing Date: 2002-12-03
Classification: B01D,B01J,G01N

Abstract:
A method for separating hemoglobin A2 from a hemoglobin mixture according to a cation exchange liquid chromatographic technique, characterized that at least two types of eluents are used including an eluent (hereinafter referred to as â€œeluent Aâ€ ) for elution of faster hemoglobins than hemoglobin A0 and an eluent (hereinafter referred to as â€œeluent Bâ€ ) for elution of hemoglobin A0 and slower hemoglobins than hemoglobin A0 and that said eluent A has a pH of 4.0-6.0 and said eluent B is maintained at a pH of at least 0.5 higher than that of the eluent A.