Patent ID: 6682903
Filing Date: 2004-01-27
Classification: G01N

Abstract:
A method for detecting an analyte in solution comprisinga) combining i) a solution to be assayed for the presence or amount of the analyte; ii) a first ligand that binds the analyte, wherein said first ligand is directly or indirectly bound to a hydrolase that cleaves a first substrate to produce a colorless first product; iii) a second ligand that binds the analyte, wherein the second ligand is directly or indirectly bound to an oxidase that oxidises a second substrate to produce a colorless second product; iv) said first substrate; v) said second substrate; vi) a compound that is a scavenger for the first product or the second product in step (a); and, whereby the hydrolase cleaves the first substrate to produce the first product and the oxidase oxidizes the second substrate to produce the second product, wherein the first product and the second product chemically combine, forming a covalent chemical linkage between the first product and second product, to produce a detectable reaction product, said detectable reaction product being a colored reaction product; b) detecting the production of the colored reaction product; c) relating the production of the colored reaction product with the presence or amount of analyte in the solution.