Patent ID: 6168929
Filing Date: 2001-01-02
Classification: C07K,C12N,C12Q,G01N,Y10S

Abstract:
A method of identifying an agent that interferes with the interaction of a J-domain and a DnaK protein comprising: contacting a polypeptide comprising the J-domain of SV40 T antigen with a DnaK protein in the presence of ATP and in the absence of an agent to be tested for its ability to block the stimulation of ATPase activity; determining the stimulation of ATPase activity of the DnaK protein by the polypeptide comprising the J-domain of SV40 T antigen; contacting a polypeptide comprising the J-domain of SV40 T antigen with the DnaK protein in the presence of ATP and in the presence of an agent to be tested for its ability to block the stimulation of ATPase activity; determining the stimulation of ATPase activity of the DnaK protein by the polypeptide comprising the J-domain of SV40 T antigen in the presence of the agent to be tested; and comparing the stimulation of ATPase activity in the absence and presence of the agent to be tested, wherein a decrease in ATPase activity in the presence of the agent indicates an agent that interferes with the activation of a DnaK protein by a protein comprising a J-domain.