Patent ID: 6461827
Filing Date: 2002-10-08
Classification: G01N,Y10S

Abstract:
A method for detecting or predicting an ischemic disorder in a subject, the method comprising the following steps:(a) providing a first and a second body fluid sample, wherein the first sample is taken from a test subject from which an ischemic disorder is to be detected or predicted and the second sample is taken from a normal subject, and the body fluid sample is selected from the group consisting of a blood sample, a urine sample, a saliva sample and a semen sample; (b) providing a first antibody and a second labeled antibody, wherein both the first and the second antibodies are capable of specifically binding to a human lipocalin-type prostaglandin D synthase, and a detecting reagent capable of detecting the label; (c) contacting the first antibody, the second labeled antibody, and the detecting reagent with the first body fluid sample; (d) contacting the first antibody, the second labeled antibody, and the detecting reagent with the second body fluid sample; and, (e) detecting the amount of and determining the concentration of human lipocalin-type prostaglandin D synthase in the normal subject sample to provide a reference value and detecting the amount of and determining the concentration of human lipocalin-type prostaglandin D synthase in the test subject sample, wherein an increased concentration of human lipocalin-type prostaglandin D synthase in the test subject sample compared to the reference value indicates that the test subject has a high probability of having had or being at risk of having an ischemic disorder.