Patent ID: 7560269
Filing Date: 2009-07-14
Classification: C12Q,G01N

Abstract:
1. A device for monitoring cell-substrate impedance, comprising: a) a nonconducting substrate; b) two or more electrode arrays fabricated on a same plane of said substrate, wherein each of said two or more electrode arrays comprises two electrode structures and the electrode structures of each of said two or more electrode arrays have substantially the same surface area; c) two or more fluid containers on said substrate, wherein each of said two or more arrays is associated with one of said two or more fluid containers; and d) at least two connection pads: wherein for each of said two or more electrode arrays, each of said two electrode structures comprises multiple electrode elements and said multiple electrode elements of each of said two electrode structures are connected to an electrode bus, further wherein the first of said two electrode structures of each of said at least two electrode arrays is connected to one of said at least two connection pads from a first electrode bus via an electrically conductive trace, and the second of said two electrode structures of each of said at least two electrode arrays is connected to another of said at least two connection pads from a second electrode bus via another electrically conductive trace, further wherein each array is individually addressed; further wherein for at least two of said two or more electrode arrays, each of which has a first electrode structure that is connected to a common connection pad from the first electrode bus via the electrically conductive trace and a second electrode structure that is connected to an independent connection pad from the second electrode bus via the another electrically conductive trace; further wherein electrode buses are positioned at ends of electrically conductive traces and not within electrically conductive traces as trace segments; and further wherein said substrate has a surface suitable for cell attachment or growth; wherein said cell attachment or growth on said substrate can result in a detectable change in impedance between or among said electrode structures within each electrode array.