Patent ID: 7824913
Filing Date: 2010-11-02
Classification: A61K,A61P,C12N

Abstract:
1. A method for producing a cultured tissue construct, comprising, (a) seeding human fibroblast cells capable of synthesizing an extracellular matrix on a porous membrane, wherein the membrane comprises pores that are about 3 microns or less in size, in a culture vessel in a chemically defined cell culture medium, wherein the chemically defined cell culture medium is free of undefined animal organ or tissue extracts and comprises the following: (b) stimulating the fibroblast cells, which are at least at 80% confluence to synthesize, secrete and organize extracellular matrix components; and, (c) continued culturing of the fibroblast cells to thereby produce a cultured tissue construct with an extracellular matrix of at least about 30 microns thick and cultured fibroblast cells contained within the synthesized extracellular matrix layer, wherein the extracellular matrix comprises: and wherein said extracellular matrix is produced by the cultured fibroblast cells on one surface of the porous membrane.