Patent ID: 8911944
Filing Date: 2014-12-16
Classification: C12Q,G01N

Abstract:
1. A method for identifying a polymorphism of nucleic acid molecules comprising: (a) preparing a sample solution comprising a first nucleic acid probe, which is labeled with a fluorescent substance and specifically hybridizes with a single-stranded nucleic acid molecule comprising a first type of base sequence in a polymorphic sequence, and a target nucleic acid molecule; (b) hybridizing nucleic acid molecules in the sample solution prepared in (a); (c) calculating the number of molecules of hybrids comprising the first nucleic acid probe in the sample solution prepared in (a) after carrying out (b) that comprises: (d) preparing a sample solution comprising a second nucleic acid probe, which is labeled with a fluorescent substance and in which the base sequence thereof differs from the base sequence of the first nucleic acid probe, and the target nucleic acid molecule; (e) hybridizing the nucleic acid molecules in the sample solution prepared in (d); (f) calculating the number of molecules of hybrids comprising the second nucleic acid probe in the sample solution prepared in (d) after carrying out (e) that comprises: (g) identifying a polymorphism of the target nucleic acid molecule based on the results of (c) and (f).