Patent ID: 6794132
Filing Date: 2004-09-21
Classification: A01K,C07K,C12N,C40B

Abstract:
A method of producing a human antibody display library, comprising:providing a transgenic mouse whose genome comprises a plurality of human immunoglobulin genes that can be expressed to produce a plurality of human antibodies, wherein the transgenic mouse comprises less than the full complement of human immunoglobulin genes present in a human being; isolating a population of nucleic acids encoding human antibody chains from lymphatic cells of the transgenic mouse by amplifying the population of nucleic acids using a set of primers selected based on which human immunoglobulin genes from the full complement of human immunoglobulin genes are present in the genome of the transgenic mouse; forming a library of display packages displaying the antibody chains, wherein a library member comprises a nucleic acid encoding an antibody chain, and the antibody chain is displayed from the package, wherein the library comprises at least 100 members at least 50% of which comprise nucleic acids encoding human antibody chains showing at least 109Mâˆ’1 affinity for the same target and no library member constitutes more than 50% of the library.