Patent ID: 6030779
Filing Date: 2000-02-29
Classification: C12N,C12Q

Abstract:
A method for identifying a desired activity encoded by a genomic DNA population comprising:(a) obtaining a single-stranded genomic DNA population;(b) contacting the single-stranded DNA population of (a) with a DNA probe tunder conditions and for sufficient time to allow hybridization and to produce a double-stranded complex of probe and members of the genomic DNA population which hybridize thereto;(c) separating the complex from the single-stranded DNA population of (b);(d) releasing from the probe the members of the genomic population which had been bound to the probe;(e) forming double-stranded DNA from the members of the genomic population of (d);(f) introducing the double-stranded DNA of (e) into a suitable host cell to produce an expression library containing a plurality of clones containing the selected DNA; and(g) screening the expression library for the desired activity.