Patent ID: 6114699
Filing Date: 2000-09-05
Classification: G01N

Abstract:
A method for determining total dietary fiber by NIRS in a sample containing one or more grains, said method comprising:(a) obtaining grain samples having >10% fat and having known dietary fiber content and representative of the range of dietary fiber to be determined;(b) milling each of said samples to form milled samples;(c) irradiating each of said milled samples with near infrared radiation and digitally recording reflectance intensity at discrete frequencies;(d) comparing variations in spectral intensity at said discrete frequencies with known total dietary fiber content for said variety of samples and selecting frequencies at which spectral deviations correlate with total dietary fiber content;(e) developing a calibration model based on the results of step (d);(f) obtaining a grain sample having >10% fat of which the dietary fiber content is to be determined;(g) milling said sample of step (f) to form a milled sample;(h) irradiating the milled sample of step (g) with near infrared radiation spectrometer and digitally recording spectral absorbance; and(i) determining total dietary fiber by comparing the scan with said calibration model.