Patent ID: 6573055
Filing Date: 2003-06-03
Classification: C07K,G01N

Abstract:
A method of detecting in a body fluid of a mammal a recombinant erythropoietin produced by cells of species different from the mammal, wherein the cells are CHO cells, comprising the steps of:raising heterologous antibodies to the recombinant erythropoietin produced in CHO cells wherein the antibodies bind to a carbohydrate-containing epitope present on the recombinant erythropoietin but not present on erythropoietin produced by the mammal and wherein the antibodies do not bind to erythropoietin produced by the mammal; contacting a body fluid of the mammal with the antibodies under conditions sufficient to allow binding of the antibodies; and detecting binding of the antibodies wherein binding of said antibodies is indicative of presence of the recombinant erythropoietin in the mammal.