Patent ID: 8741577
Filing Date: 2014-06-03
Classification: G01N

Abstract:
1. A biologically-functional, surface-immobilized multilayer structure, comprising (i) a support surface, (ii) a plurality of first vesicles formed of a lipid bilayer membrane and spaced apart from said surface, and (iii) a plurality of second vesicles formed of a lipid bilayer membrane and spaced apart from said surface, wherein the plurality of first vesicles is directly attached to the surface by binding surface-immobilized linkers with vesicle-attached linkers to form a first immobilized layer of the plurality of first vesicles, wherein the plurality of the second vesicles is attached to the first immobilized layer of the plurality of first vesicles by binding vesicle-attached linkers to vesicle-attached linkers to form two or more immobilized layers of the plurality of second vesicles, wherein the surface-immobilized linkers and the vesicle-attached linkers comprise oligonucleotides and binding of one linker to another linker is mediated through hybridization of said linker oligonucleotides; and wherein at least a selected population of said vesicles comprise a biologically active compound which provides the structure with biological functionality.