Patent ID: 8703070
Filing Date: 2014-04-22
Classification: B01L,G01N,Y10S,Y10T

Abstract:
1. An immunoassay test apparatus, comprising: a cartridge, capable of rotating about a reference axis, and having at least one test unit, wherein the test unit comprises: a plurality of fluid chambers, wherein each of the fluid chambers stores a working fluid, a plurality of pin chambers, wherein each of the pin chambers is corresponding to one of the fluid chambers, each of the pin chambers is configured with a first sealing film, and the first sealing film is used for preventing the pin chamber from communicating with the fluid chamber, a microchannel structure, communicating each of the pin chambers, wherein the microchannel structure is used for guiding the working fluid to flow from the fluid chamber to a detection chamber, a buffer chamber, connected to the microchannel structure, the detection chamber, in communication with the buffer chamber, a waste chamber, in communication with the detection chamber, a capillary U-shaped guiding groove, connecting the detection chamber and the waste chamber, and a gas exhaust structure, connected to the buffer chamber and the plurality of fluid chambers; a pin-film assembly, comprising: a second sealing film, disposed on and covering one surface of the cartridge where the detection chamber is disposed, a plurality of pierce mechanisms, disposed on the second sealing film, wherein each of the pierce mechanisms is corresponding to one of the pin chambers, each of the pierce mechanisms comprises a pin and a flexible structure, and the flexible structure is arranged for allowing the pin to move from a first position to a second position, and a first actuation unit, for driving the pin to move from the first position to the second position, wherein upon moving from the first position to the second position, the pin pierces the first sealing film, so that the pin chamber is in communication with the fluid chamber; a plurality of magnetic particles, disposed inside the detection chamber, wherein each of the magnetic particles is capable of magnetic attraction, and each of the magnetic particles has a first marked substance on a surface thereof; at least one first magnetic unit, disposed on one side of the detection chamber, wherein the first magnetic unit is used for producing a magnetic field on the plurality of magnetic particles, so as to draw and attach the plurality of magnetic particles to the side of the detection chamber where the first magnetic unit is disposed; and at least one second magnetic unit, capable of moving reciprocatingly between a third position and a fourth position, wherein a magnetic force of the second magnetic unit is larger than that of the first magnetic unit, and when the second magnetic unit is located at the fourth position, the second magnetic unit is located on one side of the detection chamber opposite to the first magnetic unit, and the second magnetic unit produces a magnetic field on the plurality of magnetic particles, so as to draw and attach the plurality of magnetic particles to the side of the detection chamber where the second magnetic unit is disposed.