Patent ID: 8722587
Filing Date: 2014-05-13
Classification: A61K,A61P,C07K

Abstract:
1. A method for preparing nucleotides of single-chain variable fragments (scFv) encoding an antigen-specific binding domain, comprising the steps of: a) isolating RNA encoding an antibody from splenocytes or lymphocytes of a non-immunized canine, or a canine immunized with a specific antigen; b) generating cDNAs of the isolated RNA; c) amplifying the variable regions of the antibody's heavy chain and the lambda and kappa light chains to generate V wherein the set of primers include at least one pair of V wherein the at least one pair of V wherein the at least one V wherein the at least one V wherein the at least one V wherein the primers are designed to incorporate secondary primer binding sites into the 5′ end of the V d) using the flexible linker, randomly linking the V