Patent ID: 8927276
Filing Date: 2015-01-06
Classification: C12N

Abstract:
1. A method for propagating central nervous system multipotent progenitor and stem cells (NSCs) and their differentiation into neuronal cells, the method comprising: a) initiating a growth period by culturing NSCs in a serum-free medium comprising epidermal growth factor (EGF), b) culturing the NSCs from step a) in a proliferation medium comprising serum and epidermal growth factor (EGF), fibroblast growth factor (bFGF) and leukemia inhibitory factor (LIF); wherein the cultured conditions from steps a) and b) are alternated every 5 to 7 days, and c) differentiating the cells of b) by culturing the cells on medium containing no growth factors on a laminin coated matrix for cell attachment, wherein NSCs differentiate into neuronal cells.