Patent ID: 6165774
Filing Date: 2000-12-26
Classification: A61K,A61P,C07K,C12N,C12Y

Abstract:
A method of producing a coisolated and copurified mixture of glycoproteins of parainfluenza virus type 1 (PIV-1), which comprises:growing PIV-1 in a cell culture;separating the grown virus from the cell culture;solubilizing the hemagglutinin-neuraminidase (HN) and the fusion (F) envelope glycoproteins from the separated virus; andcoisolating and copurifying the solubilized envelope glycoproteins by:collecting HN and F glycoprotein-containing flow-through from ion exchange chromatograghy of the solubilized envelope glycoproteins,loading the flow through onto a hydroxyapatite matrix, andselectively coeluting the HN and F glycoproteins from the hydroxyapatite matrix.