Patent ID: 7348008
Filing Date: 2008-03-25
Classification: A61K,A61P,C07K,Y02A

Abstract:
1. A method of inhibiting or destroying cells having a specific protease comprising contacting the cells with an effective amount a recombinant protein comprising an A chain of a ricin-like toxin, a B chain of a ricin-like toxin and a heterologous linker amino acid sequence, linking the A and B chains, wherein the linker sequence contains a cleavage recognition site for a specific protease and is selected from the group consisting of the amino acid sequence of PAP302 as shown in FIG. 2C (SEQ ID NO:14); the amino acid sequence of PAP303 as shown in FIG. 3C (SEQ ID NO:21): the amino acid sequence of PAP304 as shown in FIG. 4C (SEQ ID NO:28); the amino acid sequence of PAP305 as shown in FIG. 5C (SEQ ID NO:35); the amino acid sequence of PAP308 as shown in FIG. 6C (SEQ ID NO:42): the amino acid sequence of PAP313 as shown in FIG. 8C (SEQ ID NO:56); the amino acid sequence of PAP316 as shown in FIG. 11C (SEQ ID NO:77); the amino acid sequence of PAP323 as shown in FIG. 16C (SEQ ID NO:112); the amino acid sequence of PAP324 as shown in FIG. 17C (SEQ ID NO:119); and the amino acid sequence of PAP325 as shown in FIG. 18C (SEQ ID NO:126).