Patent ID: 6531579
Filing Date: 2003-03-11
Classification: C07K,C12N

Abstract:
Isolated fused polypeptide comprising amino acid residues selected from the group consisting of:(a) SEQ ID NO:24; (b) SEQ ID NO:24 with one to five conservatively substituted amino acid residues; (c) SEQ ID NO:24 with an addition or deletion of one to five amino acid residues; (d) an amino acid sequence encoded by a nucleic acid sequence which hybridizes under stringent conditions to the complement of nucleotides 161-940 of the nucleotide sequence SEQ ID NO:1 and which modulates hedgehog signaling; (e) SEQ ID NO:2; (f) SEQ ID NO:2 with one to five conservatively substituted amino acid residues; (g) SEQ ID NO:2 with an addition or deletion of one to five amino acid residues; and (h) an amino acid sequence encoded by a nucleic acid sequence which hybridizes under stringent conditions to the complement of nucleotides 161-1315 of the nucleotide sequence SEQ ID NO:1 and which modulates hedgehog signaling; and wherein said stringent conditions are defined by: (i) 0.015M sodium chloride, 0.0015M sodium citrate, 0.1% sodium dodecyl sulfate at 50Â° C.; or (ii) 50% vol./vol. formamide with 0.1% bovine serum albumin, 0.1% Ficoll, 0.1% polyvinylpyrrolidone, 50 mM sodium phosphate buffer at pH 6.5 with 750 mM sodium chloride, 75 mM sodium citrate at 42Â° C.; or (iii) 50% formamide, 5Ã—SSC (0.75 M NaCl, 0.075M sodium citrate), 50 mM sodium phosphate (pH 6.8), 0.1% sodium pyrophosphate, 5Ã—Denhardt's solution, sonicated salmon sperm DNA (50 &mgr;g/ml), 0.1% SDS, 10% dextran sulfate at 42Â° C., wherein (i), (ii) or (iii) is followed with washes at 42Â° C. in 0.2Ã—SSC and 50% formamide at 55Â° C., followed by a wash of 0.1Ã—SSC containing EDTA at 55Â° C.