Patent ID: 7435556
Filing Date: 2008-10-14
Classification: C07K,C12N

Abstract:
1. A method for increasing the ratio of p-hydroxybenzoic acid (pHBA) ester glucoside to total pHBA glucose conjugates in pHBA-producing microorganisms or green plant cells, the method comprising: (a) transforming a pHBA-producing microorganism or green plant cell with a nucleic acid fragment encoding a polypeptide having UDP-glucosyltransferase activity operably linked to suitable regulatory sequences, the polypeptide having (b) growing the transformed microorganism or green plant cell of step (a) under suitable conditions for expressing UDP-glucosyltransferase activity and producing pHBA ester glucoside; and (c) recovering pHBA ester glucoside, the ratio of pHBA ester glucoside to total pHBA glucose conjugates at least 10% greater than the ratio of pHBA ester glucoside to total pHBA glucose conjugates produced by the corresponding untransformed microorganisms or green plant cells.