Patent ID: 7601495
Filing Date: 2009-10-13
Classification: C07H,C12N,C12P,C12Q

Abstract:
1. A method for quantifying a first small nucleic acid in a reaction mixture comprising the first small nucleic acid and a second small nucleic acid, wherein the first small nucleic acid and the second small nucleic acid differ by only two nucleotides, wherein the small nucleic acids are 18-25 nucleotides in length, said method comprising; selectively forming an extension reaction product of the first small nucleic acid, wherein a 3′ target-specific portion of a linker probe hybridizes to the first small nucleic acid but not to the second small nucleic acid, wherein the extension reaction product is created by extending from the 3′ target-specific portion of the linker probe; exponentially amplifying the extension reaction product of the first small nucleic acid in a polymerase chain reaction (PCR); and selectively quantifying the amplified extension reaction product of the first small nucleic acid over the second small nucleic acid by hybridizing a detector probe to a sequence corresponding to the first small nucleic acid.