Patent ID: 6251600
Filing Date: 2001-06-26
Classification: C07H,C12Q

Abstract:
A process for amplifying a target nucleic acid sequence comprising the steps of:a) providing a nucleotide amplification reaction mixture containing the target nucleic acid sequence configured to amplify the target nucleic acid sequence;b) within the nucleotide amplification reaction mixture, contacting the target nucleic acid sequence with a first probe that is complementary to the target nucleic acid sequence and comprises at least one releasable probe portion, wherein the first probe and the target nucleic acid sequence form a duplex having an enzymatically cleavable region wherein enzymatic cleavage of the first probe results in the release of a releasable probe portion; andc) contacting the first probe and target nucleic acid sequence duplex with an enzyme capable of cleaving the enzymatically cleavable region to release a releasable probe portion andd) making sequential measurements of the release of the releasable probe portion to kinetically characterize the target DNA sequencewherein steps b)-d) are performed in a single reaction mixture.