Patent ID: 6593113
Filing Date: 2003-07-15
Classification: C12Q

Abstract:
An in vitro method for providing templates for DNA sequencing, comprising the steps ofcarrying out a transposition reaction in the presence of a target DNA and in the presence of a transposon, said transposition reaction resulting in at least one insertion into said target DNA; performing an amplification reaction by using as primers a first, fixed primer hybridizing at a known location in said target DNA or, if the target DNA is part of a vector, adjacent to said target DNA, and a second, selective primer hybridizing at the transposon insertion site, said selective primer having a sequence complementary to the joining end of said transposon and having additional nucleotides of known identity at its 3â€² end; separating the amplification products on the basis of their size, and recovering said amplification products suitable for use as templates in sequencing said target DNA.