Patent ID: 9206263
Filing Date: 2015-12-08
Classification: A01K,C07K,C12N

Abstract:
1. A method for making an antibody comprising a human λ variable domain, the method comprising the steps of: (a) exposing a genetically modified mouse to an antigen, wherein the genetically modified mouse has a genome that comprises an endogenous κ light chain immunoglobulin locus comprising a replacement of endogenous Vκ and Jκ gene segments with human Vλ and Jλ gene segments, wherein the human Vλ and Jλ gene segments are operably linked to a mouse Cκ gene such that the mouse expresses an immunoglobulin light chain that comprises a human λ variable sequence fused with a mouse κ constant region; (b) allowing the genetically modified mouse to develop an immune response to the antigen; and (c) isolating from the mouse of (b) an antibody specific for the antigen, wherein the antibody comprises a human λ variable domain derived from the human Vλ and Jλ gene segments.