Patent ID: 6060644
Filing Date: 2000-05-09
Classification: C07K,C12N

Abstract:
A method of introducing a nucleic acid into a plant cell, which method comprises the steps of:(a) inserting into a plant cell (i) a sense nucleic acid, wherein said sense nucleic acid encodes a product of a cuticular lipid gene to be expressed in said plant cell, or (ii) an antisense nucleic acid, wherein said antisense nucleic acid expresses an antisense nucleic acid molecule, which is specific for mRNA or a portion thereof of a cuticular lipid gene present in and expressed in said plant cell, wherein said antisense nucleic acid molecule is of sufficient length to inhibit expression of said cuticular lipid gene,wherein said sense or antisense nucleic acid is operatively linked to one or more regulatory sequences, andwherein said cuticular lipid gene comprises a sequence selected from the group consisting of SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 17, SEQ ID NO: 30, SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 19, SEQ ID NO: 20, SEQ ID NO: 23 and a sequence which hybridizes to any of the aforementioned nucleic acid sequences under stringent conditions;(b) expressing said sense or antisense nucleic acid in said plant cell; and(c) culturing said plant cell.