Patent ID: 7906276
Filing Date: 2011-03-15
Classification: C12Q,G01N

Abstract:
1. A method for detecting an enzyme within a test sample, the method comprising: contacting a test sample with a plurality of reactive complexes to form an incubation mixture, the reactive complexes each comprising a substrate joined to a reporter and specific binding member, wherein the substrate is cleaved by the enzyme to release the reporter and the specific binding member; applying the incubation mixture to a chromatographic medium so that the released specific binding member flows through the chromatographic medium to a first detection zone, the chromatographic medium comprising the first detection zone within which a first receptive material is immobilized, the first receptive material having an affinity for the specific binding member, wherein the chromatographic medium further comprises a second detection zone within which a second receptive material is immobilized, the second receptive material comprising a biological material and having an affinity for the reporter or complexes thereof, the reporter being capable of directly or indirectly generating a first detection signal within the first detection zone and a second detection signal within the second detection zone; and determining the presence or intensity of the first detection signal within the first detection zone and the second detection signal within the second detection zone, wherein the amount of an enzyme within the test sample is inversely proportional to the intensity of the first detection signal and directly proportional to the intensity of the second detection signal.