Patent ID: 6803224
Filing Date: 2004-10-12
Classification: C12N

Abstract:
A cascade genetic circuit comprising one or more nucleic acid constructs that comprise the components:a) a gene encoding a Nah-R transcriptional regulator; wherein said Nah-R transcriptional regulator is responsive to one or more benzoate derivatives; b) a Psal promoter, wherein in the presence of at least one of said one or more benzoate derivatives, said Psal promoter is activated by said Nah-R transcriptional regulator; c) a gene encoding the XylS2 transcriptional regulator, wherein said gene encoding the XylS2 transcriptional regulator is regulated by said Psal promoter, and further wherein said XylS2 transcriptional regulator is responsive to at least one of said one or more benzoate derivatives; and d) a Pm promoter, wherein said Pm promoter is responsive to said XylS2 transcriptional regulator.