Patent ID: 8632993
Filing Date: 2014-01-21
Classification: A61P,G01N

Abstract:
1. A method for determining the presence of grass species extracts in a grass pollen extract composition, comprising: subjecting a sample of grass pollen extract to proteolytic treatment that cleaves peptide chains at the carboxyl side of the amino acids lysine and arginine, except when either is followed by proline, to obtain a mixture of peptides; subjecting the mixture of peptides to an analytical chemistry technique to specifically determine the presence of peptides, within the mixture of peptides, that consist of the amino acid sequences of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4 and SEQ ID NO: 5; determining the presence of pollen from each of Perennial rye, Timothy, Orchard, Sweet vernal, and Kentucky bluegrass if a peptide consisting of the amino acid sequence of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4 or SEQ ID NO: 5, respectively, is detected in the sample.