Patent ID: 7390629
Filing Date: 2008-06-24
Classification: A61K,A61P,C07K

Abstract:
1. A method for identifying a tumor-activated prodrug-form of a therapeutic agent, the therapeutic agent having desired activity in a tumor cell which secrets an enzyme secreted by MCF7/6, HepG2, or HT29 cells, wherein the tumor-activated prodrug is capable of providing the therapeutic agent selectively to the tumor cell in the presence of a normal cell, the method comprising: (a) identifying a substrate of an enzyme that is secreted by MCF7/6, HepG2, or HT29 cells, wherein the enzyme is not secreted by normal cells; (b) covalently attaching the substrate to the therapeutic agent to form a prospective tumor-activated prodrug; (c) testing to determine: (d) identifying as a tumor-activated prodrug any prospective tumor-activated prodrug that meets the following conditions: