Patent ID: 8084399
Filing Date: 2011-12-27
Classification: C07K

Abstract:
1. A method of screening an amphiphilic peptide binding specifically to a target hairpin RNA for production of a diagnostic reagent for hepatitis C virus comprising the steps of: 1) constructing an amphiphilic peptide library containing the amphiphilic peptide consisting of the amino acid sequences of SEQ. ID. NO: 1-No: 37; 2) synthesizing Internal Ribosome Entry Site (IRES) RNA as the target hairpin RNA of hepatitis C virus; 3) calculating binding force between RNA and the amphiphilic peptide by measuring fluorescent anisotropy of a mixture composed of the amphiphilic peptide, hairpin RNA and a fluorescent compound by using a fluoro spectrophotometer; and 4) selecting the amphiphilic peptide, wherein said amphiphilic peptide has a dissociation constant less than 4.3 nM or a differentiation coefficient more than 4.9.