Patent ID: 6045995
Filing Date: 2000-04-04
Classification: C12Q,G01N

Abstract:
A method for detecting a target oligonucleotide having a sequence of nucleoaidic bases, comprising the steps of:selecting a first mixture suspected of containing said target oligonucleotide;forming a second mixture by contacting said first mixture with an oligomer having a sequence of nucleosidic bases that is complementary to at least a portion said sequence of nucleosidic bases of said target oligonucleozide and wherein said oligomer is of a known concentration, to form a hybrid species of said oligomer and said oligonucleotide;loading at least a portion of said second mixture onto a capillary electrophoresis column;performing capillary electrophoresis on said column for a time and under conditions effective to separate hybridized moieties formed between said target oligonucleotide and said oligomer from unhybridized oligomer;detecting said hybridized moieties, thereby detecting said target oligonucleotide;quantitating the amount of hybridized moieties contained in said portion of said second mixture; andrelating said amount of hybridized moieties to the amount of said target oligonucleotide contained in said first mixture,wherein the amount of hybridized moieties contained in said portion of said second mixture is quantitated using a standard of known concentration to provide an absolute quantitation;and wherein said quantitating step is performed using an internal standard.