Patent ID: 6242568
Filing Date: 2001-06-05
Classification: A61K,A61P,C07K,C12N,C12Q,G01N

Abstract:
A method for isolating a zinc finger-nucleotide binding polypeptide variant which binds to a cellular nucleotide sequence comprising:a) identifying the amino acids in a zinc finger-nucleotide binding polypeptide that bind to a first cellular nucleotide sequence and modulate the function of a nucleotide sequence;b) creating an expression library encoding the polypeptide variant containing randomized substitution of the amino acids identified in step a) above;c) expressing the library in a suitable host cell; andd) isolating a clone that produces a polypeptide variant that binds to a second cellular nucleotide sequence and modulates the function of the second nucleotide sequence;wherein the variant is comprised of at least two zinc finger modules and wherein the amino acid sequence of each module that binds the second nucleotide sequence comprises two cysteines and two histidines whereby both cysteines are amino proximal to both histidines and wherein each of two modules of said variant has at least one amino acid sequence modification.