Patent ID: 6750008
Filing Date: 2004-06-15
Classification: A61K,A61P,C07K,C12N,G01N

Abstract:
A method for identifying a compound that inhibits formation of or disrupts a DP107-like peptide/DP178-like peptide complex, said method comprising:(a) contacting, both in the presence and in the absence of a test compound, (i) a DP107-like peptide, wherein the DP107-like peptide is selected from the group consisting of DP107 (SEQ ID NO:16) and M41&Dgr;178 (SEQ ID NO:1649), and (ii) a DP178-like peptide consisting of 16 to 39 amino acid residues in length, further comprising 16 to 36 amino acid residues of the DP178 amino acid sequence (SEQ ID NO:15), wherein the peptide has one, two or three amino acid residue substitutions to the DP178 sequence, further wherein the peptide has reduced binding affinity for the DP107-like peptide relative to that of DP178 (SEQ ID NO:15); and (b) determining the binding affinity of the DP178-like peptide and the DP107-like peptide in both the presence and in the absence of the test compound under conditions sufficient for binding of the peptides, wherein a lower binding affinity in the presence of the test compound indicates that the test compound inhibits formation of or disrupts a DP107-like/DP178-like complex, and wherein the DP107-like peptide and the DP-178-like peptide each comprise an amino acid sequence identified by one or more of the ALLMOTI5, 107Ã—178Ã—4 or PLZIP sequence search motifs.