Patent ID: 7851163
Filing Date: 2010-12-14
Classification: G01N

Abstract:
1. A method for determining the effective parathyroid hormone activity in a sample, comprising the steps of: (A) determining the agonist activity in a sample by reacting the sample with a first antibody which recognizes an epitope between amino acids 15 to 22 of the human parathyroid hormone (SEQ ID NO:16); reacting the sample with a second antibody which recognizes an epitope that is formed of the N-terminal amino acids 1-3 of the parathyroid hormone (SEQ ID NO:16) and contains the N-terminal amino acids; and determining the number of molecules which are recognized by the first and second antibodies; and (B) determining the antagonist activity of a sample by reacting the sample with a third antibody which binds to the parathyroid hormone sequence (SEQ ID NO:16) between amino acids 4 to 14; reacting the sample with a fourth antibody which binds to the parathyroid hormone sequence (SEQ ID NO:16) between amino acids 15 to 37; and determining the number of molecules which are recognized by the third and fourth antibodies; (C) placing the concentration of PTH molecules which are bound by the first and second antibodies, and the third and fourth antibodies, respectively, into a 1:1 relationship with one another to determine the physiologically effective parathyroid hormone activity; and (D) calculating the physiologically effective parathyroid hormone activity using the following equation: