Patent ID: 7611909
Filing Date: 2009-11-03
Classification: C07D,G01N,Y10S,Y10T

Abstract:
1. An assay method for the detection or quantitation of a biological molecule in a biological sample comprises binding a binding partner with a tracer to form a binding complex between the binding partner and the tracer, and wherein chemiluminescence is triggered from said tracer following said binding, wherein the improvement comprising detecting a light signal emitted at a wavelength maximum longer than 590 nm from said binding complex, and relating said detected light signal to the presence or an amount of said biological molecule in said biological sample, wherein said tracer is formed by covalently coupling, either directly or indirectly, a near-infrared acridinium compound to said biological molecule or analog, wherein said biological molecule is selected from the group consisting of small organic biomolecules, haptens and ligands, wherein said near-infrared acridinium compound comprises an acridinium nucleus having an electron-donating substituent directly attached to the acridinium nucleus, with the electron-donating substituent attached at the C wherein R R R is alkyl, alkenyl, alkynyl, aryl or aralkyl, or alkyl, alkenyl, alkynyl, aryl or aralkyl having up to 20 heteroatoms; alternatively, R C W is an electron-donating substituent that can donate an electron pair selected from the group consisting of OH, SH, NR′R″ and —CH R′, R″ and R′″ are hydrogen or low alkyl and can all be the same or different; R a counter-ion to pair with the quaternary nitrogen of the acridinium nucleus, wherein said counter-ion may be part of R X is nitrogen, oxygen or sulfur, such that when X is oxygen or sulfur, Z is omitted and Y is a polysubstituted aryl moiety of the formula: where R R R R a halide, —COOH, —Q—R—Nu, —Q—R—(I)nNu—, —Q—Nu, —R—Nu and —Nu, where n is a integer of at least 1, Nu is a nucleophilic group, Q is a functional linkage, and I is an ionic or ionizable group; and wherein R when X is nitrogen, then Z is —SO