Patent ID: 6828159
Filing Date: 2004-12-07
Classification: C09B,G01N

Abstract:
In an immunoassay or nucleic acid hybridization method for the detection of an analyte in a sample, the improvement which comprises using a labeled receptor for the analyte wherein the label is a compound of the general formula I wherein:R1, R2, R3, R4, R5, R6 and R7 are, independently, hydrogen, halogen, a hydroxyl, amino, sulfo, carboxyl or aldehyde group, a substituted or unsubstituted, saturated or unsaturated straight chain, branched or cyclic alkyl group having up to 20 carbon atoms, or a substituted or unsubstituted, aromatic ring system; or two or more adjacent R1-R7 groups, together may form a ring system which containing one or more multiple bonds; R8 and R8a are, independently, a saturated or unsaturated, straight-chain, branched or cyclic alkyl group having up to 20 carbon atoms, or R8 and R8a, together, can form a ring system of one or more rings; R9, R10, R11 and R12, are independently, hydrogen, a substituted or unsubstituted, saturated or unsaturated, straight-chain, branched or cyclic alkyl group having up to 20 carbon atoms, a polyether, substituted or unsubstituted phenyl or substituted or unsubstituted phenylalkyl having 1-3 carbon atoms in the alkyl chain, provided that any of these groups may optionally contain one or more atoms selected from the group consisting of oxygen, sulfur and nitrogen atoms; or two or more adjacent R9-R12 groups, together, may form a ring system which can contain one or more multiple bonds; with the proviso that if R1-R3 and R5-R7 are hydrogen and R8, R8a and R9-R12 are methyl, then R4 is not one of hydrogen, hydroxyl, methyl, isopropyl, t-butyl, phenyl, o-tolyl, p-tolyl, 2,6-dimethylphenyl, 2-t-butylphenyl, 2-isopropenylphenyl and 4-dimethylaminophenyl; wherein either or both of the groups â€”N(R11)(R12) and &boxH;N(R9)(R10) can be replaced by either â€”OR9 or &boxH;O; and X represents a species of anion present for charge equalization.