Patent ID: 7666655
Filing Date: 2010-02-23
Classification: C07K,C12P

Abstract:
1. A recombinant Escherichia coli bacterium that has the ability to produce and accumulate an aromatic L-amino acid, wherein the aromatic L-amino acid production by said bacterium is enhanced by enhancing activity of a protein in a cell of said bacterium beyond the levels observed in a wild-type of said bacterium, wherein said protein is as defined in the following (A), (B), or (C): (A) a protein which consists of the amino acid sequence of SEQ ID NO: 2; (B) a protein which consists of the amino acid sequence of SEQ ID NO: 2 except wherein one to five amino acids are deleted, substituted, inserted, or added; or (C) a protein which consists of the amino acid sequence that is encoded by a nucleotide sequence that hybridizes with the complement of the nucleotide sequence of SEQ ID NO: 1 under stringent conditions comprising 60° C., 1×SSC, 0.1% SDS; wherein said protein defined in (A), (B), or (C) has an activity to make said bacterium resistant to L-phenylalanine, fluoro-phenylalanine or 5-fluoro-DL-tryptophan and wherein the activity of said protein defined in (A), (B), or (C) is enhanced by: a) transformation of said bacterium with a DNA encoding said protein and expressing the protein in said bacterium, b) replacing the native promoter that precedes the DNA encoding said protein on the chromosome of the bacterium with a more potent promoter, or c) introduction of multiple copies of the DNA encoding said protein into the chromosome of said bacterium and expressing the protein in said bacterium.