Patent ID: 6576467
Filing Date: 2003-06-10
Classification: C07K,C12N,C12Q,C12Y,C40B

Abstract:
A method of producing a polynucleotide encoding an antibody chain that specifically binds to a predetermined antigen, the method comprising:(a) denaturing a population of polynucleotide segments encoding CDR regions; (b) annealing the denatured population of CDR encoding polynucleotides; (c) incubating the annealed CDR encoding polynucleotides in the presence of a polymerase, thereby forming a population of polynucleotides comprising recombinant polynucleotides encoding antibodies with novel combinations of CDR regions, which novel combinations of CDR regions are not present in an antibody encoded by the population of polynucleotide segments provided in step (a); (d) repeating steps (a) through (c); and (e) screening or selecting the population of polynucleotides to identify at least one polynucleotide that encodes an antibody chain that specifically binds to a predetermined antigen.