Patent ID: 9012142
Filing Date: 2015-04-21
Classification: C12Q

Abstract:
1. A method for detecting the presence of a first target nucleotide sequence in a sample of single stranded DNA, comprising; (a) exposing the sample to a first full-length DNA probe molecule and a first nicking endonuclease under conditions that would permit sequence-specific hybridization of the first full-length probe molecule to the first target nucleotide sequence, wherein the first full-length probe molecule comprises a sequence complementary to the first target nucleotide sequence that also includes a first recognition sequence for the first nicking endonuclease with complete complementarity between the recognition sequence and the first target nucleotide sequence, whereby a first recognition site is created for the first nicking endonuclease, whereby the first full-length DNA probe molecule is cleaved into a pair of first cleaved DNA probe molecules and the first target nucleotide sequence remains intact; (b) disassociating the pair of the first cleaved DNA probe molecules from the first target nucleotide sequence, whereby the first target nucleotide sequence is free for hybridization; (c) repeating steps (a) and (b) once or more, whereby two or more pairs of the first cleaved DNA molecules are dissociated from the first target nucleotide sequence; and (d) detecting the dissociated two or more pairs of the first cleaved DNA probe molecules, wherein the presence of the dissociated two or more pairs of the first cleaved DNA probe molecules indicates the presence of the first target nucleotide sequence in the sample.