Patent ID: 8921075
Filing Date: 2014-12-30
Classification: C12P,C12Q

Abstract:
1. A method of manufacturing a nanoparticle chain, comprising the steps of: providing a single-stranded circular DNA primer having a predetermined length, and amplifying the single-stranded circular DNA primer into at least one single-stranded DNA nanotemplate by an isothermal nucleotide amplification reaction, and fixing an end of the at least one single-stranded DNA nanotemplate to a surface of a substrate; and adding a plurality of single-stranded DNA probes, and an end of each single-stranded DNA probe being coupled to a nanoparticle, and attaching the single-stranded DNA probes to a corresponding sequence of the at least one single-stranded DNA nanotemplate to form at least one nanoparticle chain; wherein the predetermined length of the single-stranded circular DNA primer is adjusted to control an inter-nanoparticle distance of the nanoparticle chain.