Patent ID: 7122344
Filing Date: 2006-10-17
Classification: A01K,A61K,A61P,A61Q,C07K,C12N

Abstract:
1. A method of purifying rhUG comprising the steps of: a. providing a bacterial cell paste comprising bacterial cells capable of overexpressing rhUG; b. lysing the bacterial cell paste and pelleting the debris to form a supernatant; c. filtering the supernatant formed in step b through a first nominal molecular weight cut off membrane to form a first permeate; d. concentrating the first permeate formed in step c by the use of a second nominal molecular weight cut off membrane; e. loading the concentrated permeate formed in step d onto an anion exchange column to form a first eluate; f. concentrating the first eluate formed in step e by the use of a third nominal molecular weight cut off membrane to form a second concentrate; g. loading the second concentrate formed in step f onto a hydroxyapatite column to form a second eluate; h. separating host-derived proteins from the rhUG in the second eluate formed in step g to provide purified rhUG; and i. recovering the purified rhUG formed in step h.