Patent ID: 6436402
Filing Date: 2002-08-20
Classification: A61K,A61P,C12N

Abstract:
A method for making human papillomavirus (HPV) virus-like particles (VLPs) comprising the steps of:a) expressing HPV L1 or L1+L2 proteins; b) at least partially purifying the proteins; and c) subjecting the at least partially purified proteins to a maturation step wherein the maturation step is selected from the group consisting of: 1. glutathione facilitated thiol oxidation using 0.5 mM to about 10 mM glutathione; 2. glutathione facilitated thiol oxidation using 0.5 mM to about 25 mM oxidized glutathione; and 3.1 or 2 in combination with incubation at a temperature from about 30Â° C. to about 45Â° C. for about 10 to 48 hours and/or alternatively in combination with exposure of the at least partially purified protein to a metal surface.