Patent ID: 9034610
Filing Date: 2015-05-19
Classification: C12N,C12P

Abstract:
1. A method of producing monatin comprising converting tryptophan and/or indole-3-lactic acid to monatin, wherein the method comprises: (a) contacting tryptophan or indole-3-lactic acid with a first polypeptide that converts the tryptophan or indole-3-lactic acid to indole-3-pyruvate, wherein (i) when tryptophan is contacted with the first polypeptide, the first polypeptide is selected from the group consisting of an oxidoreductase of EC number 1.4.1., an oxidoreductase of EC number 1.4.3., an oxidoreductase of EC number 1.4.99., an aminotransferase of EC number 2.6.1., and tryptophan oxidase, and wherein (ii) when indole-3-lactic acid is contacted with the first polypeptide, the first polypeptide is selected from the group consisting of an oxidoreductase of EC number 1.1.1., and an oxidoreductase of EC number 1.1.3., (b) contacting indole-3-pyruvate generated in (a) with a C3 carbon source and a second polypeptide that converts the C3 carbon source and indole-3-pyruvate to 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid, wherein the second polypeptide is a lyase of EC number 4.1.3.-; and (c) contacting 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid generated in (b) with a third polypeptide that converts 2-hydroxy 2-(indol-3-ylmethyl)-4-keto glutaric acid to monatin, wherein the third polypeptide is selected from the group consisting of an oxidoreductase of EC number 1.4.1., an oxidoreductase of EC number 1.4.99., an aminotransferase of EC number 2.6.1., and lysine epsilon aminotransferase (EC 2.6.1.36).