Patent ID: 6713303
Filing Date: 2004-03-30
Classification: C12N

Abstract:
A method for adventitious root mass propagation of ginseng, camphor ginseng or wild ginseng by tissue culture, which comprises the steps of:inducing a callus by seeding any one of a 2-3 mm2 section of ginseng, camphor ginseng, or wild ginseng in a MS medium containing each of 2,4-D (2,4-dichlorophenoxy acetic acid), pichloram and NAA naphthaleneacetic acid) in an amount of 1.0-10.0 mg/L; forming an adventitious root by transferring the callus to a MS (MurashigeSkoog) medium containing 1.0-5.0 mg/L of ISA or NAA, after subculturing the induced callus in every 2-4 weeks which was propagated on a MS medium containing 0.1-5.0 mg/L of 2,4-D; propagating the above adventitious root on MS medium; seeding the propagated adventitious root in a balloon type airlift bioreactor and culturing the propagated adventitious root in a MS medium containing 3% of sugar and 1.0-10.0 mg/L of BSAA (benzo [b] selenienyl acetic acid) or IBA or NAA as a growth regulator to generate a cultured adventitious root; and mass propagating the cultured adventitious root by scaling up in a 20-50 ton bioreactor as a culture vessel.