Patent ID: 6221584
Filing Date: 2001-04-24
Classification: C12Q

Abstract:
A method for detecting a presence or an amount of telomerase activity, comprising:combining, in a reaction vessel, (1) a sample suspected to contain telomerase, (2) a first primer which is suitable as a telomerase substrate and (3) a plurality of nucleoside triphosphates, to form a reaction mixture, and incubating the reaction mixture under conditions to extend the first primer in an extension reaction by adding nucleoside triphosphates to the first primer, wherein the extension reaction is mediated by the telomerase, to produce an extension product;amplifying the extension product to produce amplification products;immobilizing the amplification products on a solid phase; andqualitatively or quantitatively detecting the amplification products immobilized in said immobilizing step, and correlating the detection with the presence or amount of telomerase activity, wherein the amplification products are detected via a labelling group which is provided on a detection probe which is hybridized with the amplification products in said detecting step.