Patent ID: 6350615
Filing Date: 2002-02-26
Classification: A01K,A61K,C07K

Abstract:
A method of screening a compound for its ability to modulate K12 protein growth promoting activity comprising incubating K12 protein, or portion thereof having growth promoting activity, with cells susceptible to the growth promoting activity of K12 protein, in the presence and absence of said compound, and assaying for an alteration in the growth rate of said cells in the presence of said compound,a reduction in the growth rate of said cells being indicative of a compound that modulates said growth promoting activity, wherein said K12 protein is selected from the group consisting of growth promoting activity and the amino acid sequence set forth in SEQ ID NO:1 and a protein having an amino acid sequence encoded by a nucleic acid sequence that hybridizes to the nucleic acid sequence set forth in SEQ ID NO:2 at 55Â° C. in 3Ã— saline/sodium citrate comprising 0.1% sodium dodecylsulfate and that remains hybridized when subjected to washing at 55Â° C. with 1Ã— SSC comprising 0.1% SDS.