Patent ID: 8574840
Filing Date: 2013-11-05
Classification: C12Q

Abstract:
1. A method of determining the sequence of a target nucleic acid molecule having an unknown sequence, comprising: contacting an RNA polymerase with a target nucleic acid molecule under sequencing conditions, wherein the sequencing conditions comprise the presence of one nucleoside triphosphate or the presence of four nucleoside triphosphates with one of the four nucleoside triphosphates present in a rate-limiting amount, wherein said RNA polymerase is immobilized on a solid substrate, wherein the target nucleic acid molecule comprises a rotation tag that is about 50 nm to about 2 microns in diameter; detecting the rotational pattern of the rotation tag; repeating the contacting and detecting steps at least one hundred times; and determining the sequence of the target nucleic acid molecule based, sequentially, on the presence or absence of a change in the rotational pattern in the presence of the one nucleoside triphosphate or the presence of the four nucleoside triphosphates with the one of the four nucleoside triphosphates present in a rate-limiting amount.