Patent ID: 6071713
Filing Date: 2000-06-06
Classification: C12N,C12P,Y10S

Abstract:
A bioprocess for preparing adipoyl-7-ACA comprising the steps of:(a) transforming cells of a strain of Penicillium chrysogenum which produces isopenicillin N with an expression vector containing DNA encoding an enzyme, having expandase activity capable of accepting adipoyl 6-APA as a substrate, an enzyme having hydroxylase activity capable of accepting adipoyl-7-ADCA as a substrate and an enzyme having acetyltransferase activity capable of accepting adipoyl 7-ADAC as a substrate;(b) culturing the transformed cells from step a) in a suitable culture medium containing an adipate feedstock, wherein said cells produce adipoyl-6-Aand(c) culturing the transformed cells producing adipoyl-6-APA of step b) under conditions suitable for expression of said DNA encoding enzyme, thereby producing the end product adipoyl-7-ACA.