Patent ID: 6800740
Filing Date: 2004-10-05
Classification: A01K,A61K,A61P,C07K,C12N,G01N

Abstract:
A variant of a native mature mammalian growth hormone, said variant having a non-naturally occurring growth hormone amino acid sequence or a covalent modification, comprising a mutation selected from the group consisting of:(a) an amino acid substitution at a residue corresponding to residue 1 of human growth hormone, numbered from the N-terminus; (b) an amino acid substitution at a residue corresponding to residue 4 of human growth hormone, numbered from the N-terminus, wherein the substituted amino acid is selected from the group consisting of R, N, D, C, Q, E, G, H, K, M, F, P, S, T, W, Y, and V; (c) an amino acid substitution at a residue corresponding to residue of 6 of human growth hormone, numbered from the N-terminus, wherein the substituted amino acid is selected from the group consisting of R, N, D, C, Q, E, G, H, I, K, M, F, P, S, T, W, Y, and V; (d) an amino acid substitution at a residue corresponding to residue 8 of human growth hormone, numbered from the N-terminus, wherein the substituted amino acid is selected from the group consisting of N, D, C, Q, E, G, H, I, L, K, F, P, S, T, W, Y, M, and V; (e) insertion of an amino acid adjacent to a residue corresponding to a residue of human growth hormone selected from the group consisting of 1, 4, 6, and 8, numbered from the N-terminus; and (f) covalent modification of a residue corresponding to a residue of human growth hormone selected from the group consisting of 1, 4, 6, and 8, numbered from the N-terminus.