Patent ID: 9090897
Filing Date: 2015-07-28
Classification: A61K,A61P,C12N

Abstract:
1. An in vitro method for producing interferon-lambda (IFN-λ), comprising the steps of: (a) contacting a first population of cells comprising dendritic cells or dendritic precursor cells with an agent that increases the level of CD8+ and/or eCD8+ conventional dendritic cells (cDCs); (b) isolating a second population of cells comprising more than 50% CD8+ and/or eCD8+ cDCs; (c) contacting the second population of cells with a double-stranded (ds) nucleic acid or analog thereof in an amount sufficient to induce IFN-λ production in CD8+ or eCD8+ cDCs, and (d) generating a population of IFN-λ producing CD8+ or eCD8+ cDCs, wherein said eCD8+ cDCs express Clec9a and/or Necl2.