Patent ID: 7635558
Filing Date: 2009-12-22
Classification: A61P,C12Q

Abstract:
1. A Single Strand DNA Conformation Polymorphism (SSCP) method for obtaining the editing profile of 5-HT2c-r mRNA, using a specific tissue sample or using a sample of a population of eukaryotic cells, characterized in that the method comprises: a) extraction of the total RNA of said sample, followed, where appropriate, by purification of the mRNA; b) reverse transcription of the RNA extracted in step a) and synthesis of the double-stranded DNA; c) PCR amplification of the DNA obtained in step b) using the following pair of primers specific for 5-HT2c-r mRNA, wherein said mRNA may be edited; PCR9 TGTCCCTAGCCATTGCTGATATGCT (SEQ ID No. 36); and PCR 10 GCAATCTTCATGATGGCCTTAGTCCG (SEQ ID No. 37); d) where appropriate, purification of the PCR products obtained in step c); e) where appropriate, quantification of the PCR products obtained in step d); f) dissociation of the double-stranded DNA to single-stranded DNA, in particular by heating followed by abrupt cooling; g) separation of the single-stranded DNA by capillary electrophoresis; h) obtaining of the editing profile by reading fluorescence and, where appropriate, acquisition of profile data by means of an exploitation system associated with a fluorescence reader and i) showing at least 13 characteristics profile of the edited or unedited forms of the5-HT2c-r mRNA.