Patent ID: 8137914
Filing Date: 2012-03-20
Classification: C07K

Abstract:
1. A method of detecting a variant form of alpha-fetoprotein (AFP) mRNA comprising: (a) combining a sample suspected of containing the variant form of AFP mRNA wherein the variant form of AFP cDNA comprises exons 1-14 of the cDNA encoding AFP in which exon 1 has been replaced by the polynucleotide sequence of exon A (SEQ ID NO:1), or a fusion of exon A and exon B (SEQ ID NO:2) with: (b) subjecting the mixture to thermocycling, and (c) determining the absence or the presence of the cDNA corresponding to at least a portion of the variant form of AFP mRNA in the thermocycled mixture, wherein the presence of the cDNA indicates that the sample comprises the variant form of AFP.