Patent ID: 6130086
Filing Date: 2000-10-10
Classification: A01K,C12N

Abstract:
A method of in vitro fertilization which comprises cultivating ova or preimplantation embryos in a medium prepared by formulating the components, which comprises the following amino acids in their free form or their pharmaceutically acceptable salt or ester form, the ester form being hydrolyzable to give a free form, but not any other amino acid:L-phenylalanine, L-tryptophan, L-lysine, L-threonine, L-valine, L-methionine, L-isoleucine, L-leucine, L-proline, glycine, L-alanine, L-tyrosine, L-histidine, L-arginine, L-taurine, L-aspartic acid, L-serine, L-asparagine, L-glutamic acid, L-glutamine and L-cystine, provided that at least a part of the L-cystine may be replaced by L-cysteine, wherein the amino acids have the following concentrations (mg/l): L-phenylalanine 0.69-13.8 L-tryptophan 0.67-13.5 L-lysine 2.44-48.8 L-threonine 1.48-29.5 L-valine 1.66-33.1 L-methionine 0.21-4.3 L-isoleucine 0.45-9.0 L-leucine 0.79-15.8 L-proline 1.17-23.4 glycine 1.16-23.2 L-alanine 2.66-53.2 L-tyrosine 0.77-15.4 L-histidine 1.63-32.5 L-arginine 1.12-1,000 L-taurine 0.39-7.8 L-aspartic acid 0.09-1.71 L-serine 0.78-15.5 L-asparagine 0.97-19.5 L-glutamic acid 1.36-27.2 L-glutamine 2.61-1,000 L-cystine 0.14-2.7.