Patent ID: 6331275
Filing Date: 2001-12-18
Classification: B01J,C12Q,C40B,G01N

Abstract:
An assay device, comprising:a) a solid support substrate, andb) a plurality of cleavable signal elements, includinga cleavable spacer, said cleavable spacer having a substrate-attaching end, a signal-responsive end, and a cleavage site intermediate said substrate-attaching end and said signal responsive end, wherein said cleavable signal elements attach through their substrate-attaching ends to said solid support substrate in a spatially addressable pattern;a signal responsive moiety;a first side member adapted to bind a first site on a chosen analyte;a second side member adapted to bind a second site of said chosen analyte;said signal responsive moiety being attached to said cleavable spacer at said signal responsive end, said first said member being attached to said cleavable spacer intermediate said signal responsive end and said cleavage site, and said second side member being attached to said cleavable spacer intermediate said cleavage site and said substrate attaching end; andthe signal responsive moiety remaining bound to the substrate after cleavage at the cleavage site only when said first and second side members are bound to said chosen analyte.