Patent ID: 6544765
Filing Date: 2003-04-08
Classification: C12N,C12Q

Abstract:
An isolated nucleic acid sequence encoding a polypeptide having oxaloacetate hydrolase activity, selected from the group consisting of:(a) a nucleic acid sequence encoding a polypeptide having an amino acid sequence which has at least 90% identity with amino acids 1-341 of SEQ ID NO: 2; (b) a nucleic acid sequence having at least 90% homology with a nucleotide sequence constituted by nucleotides 1157-1411, 1504-1651, and 1764-2383 of SEQ ID NO: 1; (c) a nucleic acid sequence which hybridizes under high stringency conditions with (i) the nucleic acid sequence of SEQ ID NO: 1, (ii) the cDNA sequence of SEQ ID NO: 1, or (iii) a complementary strand of (i) or (ii), wherein the high stringency conditions are defined as prehybridization and hybridization at 42Â° C. in 5X SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide, followed by washing three time for 15 minutes using 2X SSC, 0.2% SDS at 65Â° C.; (d) a subsequence of (a) or (b), wherein the subsequence encodes a polypeptide fragment which as oxaloacetate hydrolase activity.