Patent ID: 7910330
Filing Date: 2011-03-22
Classification: A61K,C07K,C12N,Y02A

Abstract:
1. A method for producing mRNA that produces a Plasmodium falciparum apical membrane antigen-1 (AMA-1) ectodomain or a fragment thereof when translated in a yeast cell, said method comprising: providing said yeast cell with a nucleic acid encoding said ectodomain or said fragment thereof, wherein said ectodomain comprises amino acid sequence 25-545 of SEQ ID NO:7, and wherein the fragment thereof comprises an amino acid sequence selected from the group consisting of 25-442, 97-442, and 97-545 of SEQ ID NO:7, and wherein said nucleic acid encoding said ectodomain or fragment thereof has been modified to utilize said yeast cell's codon usage, and wherein mAb 4G2 exhibits specificity for said ectodomain or said fragment thereof when said ectodomain or said fragment thereof is produced in a yeast cell; and expressing said nucleic acid in said yeast cell, thus producing the mRNA encoding the ectodomain or the fragment thereof, wherein the nucleic acid encoding said fragment encodes a peptide consisting of an amino acid sequence selected from the group of amino acid sequences consisting of amino acids 25-442, 97-442, and 97-545 of SEQ ID NO: 7.