Patent ID: 6472365
Filing Date: 2002-10-29
Classification: A61K,B82Y,G01N,Y02A,Y10S

Abstract:
A method for releasing at least one pharmaceutical agent at a site of a target comprising the steps:A binding an antibody to said target wherein said antibody is conjugated I) to a lipase wherein the lipase is able to lyse liposome, or II) to at least a first lipase component having no or less lipase activity in comparison to a corresponding lipase holoenzyme, and B) I) wherein when said antibody is conjugated to a lipase able to lyse liposomes, contacting a liposome containing said pharmaceutical agent with said antibody conjugated to a lipase such that the liposome can be lysed by the lipase, or II) wherein when said antibody is conjugated to the at least a first lipase component, a) adding a second lipase component which reconstitutes the lipase activity of said at least a first lipase component at least partially by binding to the first lipase component and, subsequently, contacting a liposome containing said pharmaceutical agent with the reconstituted lipase such that the liposome can be lysed by the reconstituted lipase activity, or b) adding a liposome containing said pharmaceutical agent to said antibody conjugated to the at least a first lipase component wherein the liposome is associated with a second lipase component able to reconstitute the lipase activity of the at least a first lipase component by binding to the first lipase component wherein said second lipase component is an integral part of the liposome.