Patent ID: 7700282
Filing Date: 2010-04-20
Classification: C12P,C12Q

Abstract:
1. A method for providing a decontaminated nucleic acid suitable for DNA 5-methylation analysis, comprising: a) contacting a DNA sample with a bisulfite reagent under conditions suitable to produce a bisulfite-treated sulfonated DNA sample, wherein unmethylated cytosines of the DNA are sulfonated, sulfonated and deaminated, or comprise a mixture of both forms, and wherein said sulfonated, or sulfonated and deaminated uracil has not been significantly desulfonated; and b) further contacting the bisulfite-treated sulfonated DNA sample with an amount of an enzyme that specifically degrades any contaminating nonsulfonated-uracil-containing nucleic acids and does not degrade sulfonated-uracil-containing nucleic acids thereby providing a decontaminated DNA sample suitable for DNA 5-methylation analysis.