Patent ID: 6265197
Filing Date: 2001-07-24
Classification: C12N,C12P,C12Y

Abstract:
A variant of a parent pullulanase, said variant exhibiting improved thermostability at a pH in the range of 4-6 compared to said parent, said parent having an amino acid sequence selected from the group consisting of (i) SEQ ID NO:1, (ii) SEQ ID NO:2, and (iii) a sequence that is at least 80% homologous to (i) or (ii) when homology is determined using GAP (version 8), using a gap creation penalty of 3.0 and a gap extension penalty of 0.1; said variant comprising at least one amino acid substitution relative to said parent, said at least one amino acid substitution corresponding to a position in SEQ ID NO:1 selected from the group consisting of A210P, V215P, L249P, K383P, S509P, T811P, G823P; or corresponding to a position in SEQ ID NO:2 selected from the group consisting of G306P, V311P, L345P, D605P, T907P, A919P.