Patent ID: 7202063
Filing Date: 2007-04-10
Classification: C12N,C12P

Abstract:
1. A process for producing rhamnolipids, said process comprising culturing Pseudomonas chlororaphis NRRL B-30761 in a first aqueous culture medium comprising about 0.3% NH 4 H 2 PO 4 , about 0.2% K 2 HPO 4 , about 0.2% carbon source, about 0.5 mg/L FeSO 4 , and about 0.1% MgSO 4 for about 24–about 48 hours at about 25°–about 30° C. with orbital shaking, and then culturing said Pseudomonas chlororaphis in a static second aqueous culture medium comprising per liter about 2% carbon source, about 0.7 g KH 2 PO 4 , about 0.9 g Na 2 HPO 4 , about 2 g NaNO 3 , about 0.4 g MgSO 4 .7H 2 O, and about 0.1 g CaCl 2 .2H 2 O for at least about 72 hours at about 20°–about 23° C., wherein said first and second aqueous culture medium contains only one source of carbon.