Patent ID: 6440677
Filing Date: 2002-08-27
Classification: B01J,C12Q,C40B

Abstract:
A method of making a nucleic acid pool comprising a plurality of different nucleic acids, said method comprising the steps of:a) providing a nucleic acid amplification template array comprising a surface to which are attached a multiplicity of at least 20 nucleic acid probes having different predetermined nucleic acid sequences, and wherein: i) each different probe is localized in a known location of said surface; ii) the density of said probes is greater than about 60 different probes per 1 cm2; and iii) said different probes have an identical terminal 3â€² nucleic acid sequence and an identical terminal 5â€² nucleic acid sequence, each comprising a primer binding site; and b) amplifying said multiplicity of probes at least about 10 fold in the presence of a primer that hybridizes to the primer site to provide said nucleic acid pool.