Patent ID: 6114158
Filing Date: 2000-09-05
Classification: C12N,C12Y

Abstract:
A non-naturally occurring recombinant DNA molecule comprising a first nucleotide sequence encoding a mature CelF cellulase, wherein said first nucleotide sequence being nucleotides 158 to 186 of SEQ ID NO:1 joined to nucleotides 298 to 1501 of SEQ ID NO:1, excluding a translation stop codon, or a second nucleotide sequence encoding a mature CelF cellulase, wherein said second sequence hybridizes to said first sequence under DNA:DNA hybridization conditions of moderate stringency, wherein the conditions of moderate stringency include hybridization and wash conditions of 50-60.degree. C., 1.times. SSC and 0.1% sodium SDS, and wherein said CelF cellulase has characteristics of an endoglucanase or cellobiohydrolase having hydrolytic activity for carboxymethylcellulose, barley .beta.-glucan, lichenin and para-nitrophenyl-.beta.-D-cellobioside.