Patent ID: 6794168
Filing Date: 2004-09-21
Classification: A01K,A62D,C12N,C12P

Abstract:
A process for oxidizing a substrate which is 1,2-dichlorobenzene, 1, 2, 4-trichlorobenzene, 3, 3â€²-dichlorobiphenyl, 2, 2â€², 4, 5, 5â€²-pentachlorobiphenyl, pentachlorobenzene or hexachlorobenzene, said process comprising the step of oxidizing said substrate with a mutant P450 monooxygenase enzyme in the presence of an electron transfer reductase and an electron transfer redoxin, wherein the enzyme is a P450cam enzyme as shown by SEQ ID NO:2, comprising a mutation at one or more of the amino acid positions selected from the group consisting of 87, 96, 98, 101, 185, 244, 247, 248, 295, 296, 395 and 396, and wherein the mutation is a substitution of an amino acid with an amino acid that has a less polar side chain, and further wherein a ring carbon of the substrate is oxidised in the oxidation reaction.