Patent ID: 7964534
Filing Date: 2011-06-21
Classification: G01N,G01R

Abstract:
1. A method for obtaining a focused library of candidate binding compounds for a protein family, wherein the members of the protein family bind a common ligand, comprising the steps of: (a) providing a ligand-probe having an antenna moiety and a ligand moiety, wherein at least one atom intervenes between the ligand moiety and an NMR visible nucleus of the antenna moiety, wherein the ligand-probe binds to the common ligand binding site of a protein, wherein the protein is a member of the protein family; (b) providing a sample comprising the protein, the ligand-probe and a second ligand under conditions wherein the ligand-probe, the second ligand and the protein form a bound complex; (c) detecting a subset of magnetization transfer signals between the antenna moiety of the ligand-probe and the second ligand in the bound complex, wherein said signals are obtained from an isotope-edited NOESY spectrum of said sample, thereby determining that the antenna moiety and second ligand are proximal in the bound complex; and (d) obtaining a population of candidate binding compounds comprising the ligand-probe, or a fragment thereof that binds to the common ligand binding site of said protein, covalently linked to one of a plurality of homologs of said second ligand, whereby the population contains binding compounds that bind to members of the protein family.