Patent ID: 6692928
Filing Date: 2004-02-17
Classification: C12Q,G01N

Abstract:
A method for evaluating whether an agent is a candidate cardiovascular agent, said method comprising:(a) culturing vascular endothelial cells containing a nitric oxide synthase (NOS) gene in the presence and absence of a predetermined amount of said agent; and (b) measuring NOS activity in said vascular endothelial cells in the presence and absence of said predetermined amount of said agent, wherein said NOS activity is measured within about 15 minutes after administration of said predetermined amount of said agent, whereby said agent is identified as a candidate cardiovascular agent when said NOS activity in said vascular endothelial cells in the presence of said predetermined amount of said agent is greater than said NOS activity in said vascular endothelial cells in the absence of said predetermined amount of said agent.