Patent ID: 7521212
Filing Date: 2009-04-21
Classification: A61P,C07H,C12P

Abstract:
1. A method for producing an oligosaccharide comprising lactose using a genetically modified cell starting with at least one internalized exogenous precursor consisting of lactose said method comprising: (i) obtaining a Lac Z comprises at least one recombinant gene encoding an enzyme capable of modifying said exogenous precursor or one of the intermediates in the biosynthetic pathway of said oligosaccharide from said exogenous precursor necessary for the synthesis of said oligosaccharide from said exogenous precursor, and also the components for expressing said gene in said cell; and (ii) culturing said cell on a carbon-based substrate in the presence of at least one said exogenous precursor, under conditions inducing the internalization according to a mechanism of active transport of said exogenous precursor by said cell and the production of said oligosaccharide by said cell, wherein said culturing comprises: (a) a first phase of exponential cell growth ensured by said carbon-based substrate, (b) a second phase of cell growth limited by said carbon-based substrate which is added continuously, wherein said precursor is added during the second phase.