Patent ID: 6335429
Filing Date: 2002-01-01
Classification: C12Q,G01N,Y10S

Abstract:
A reporter compound having the Formula V:R1-(AA)n-Asp-y-R6â€ƒâ€ƒ(V) or a biologically acceptable salt of said compound, or a tautomer of said compound, or a biologically acceptable salt of said tautomer, whereinR1 is an N-terminal protecting group; R6 is a blocking group which is selected from the group consisting of C1-12alkyloxycarbonyl, CH3(OCH2CH2)qOCOâ€”, CH3(CH2)r(OCH2CH2)sOCOâ€”, C1-12(alkylthio)carbonyl, aryl(C1-12)alkoxycarbonyl, H2NCOâ€”, (CH3)2NCOâ€”, (CH3CH2)2NCOâ€”, (CH3(CH2)v)(CH3)NCOâ€”, C1-12alkylsulfonyl, C1-12haloalkylsulfonyl, aryl(C1-12)alkylsulfonyl, Cl3CCH2OCOâ€”, unsubstituted benzoyl, benzylcarbonyl, phenylsulfonyl and tosyl; wherein q is 2-4, r is 0-5, s is 1-4, and v is 1-9; each AA independently is a residue of an &agr;-amino acid or &bgr;-amino acid, or a methyl, acetoxymethyl or methoxy ester of a carboxyl-containing &agr;-amino acid or &bgr;-amino acid; n is 0-5; and y is a Rhodamine moiety.