Patent ID: 6066497
Filing Date: 2000-05-23
Classification: C12M,Y10S

Abstract:
A cell culture apparatus, comprising:a supporting frame,a rotor releasably housing a plurality of vessels, the rotor being mounted for rotation about a substantially horizontal axis and supported by the frame; and means for effecting rotation of the rotor at a controlled speed,at least one reversible multi-channel pump, mounted on said rotor for rotation therewith,a manifold including at least one sealable external connection and a plurality of connections each in fluid communication with an individual channel of the pump,a vent coupled to each vessel and in fluid communication with the atmosphere,a dip tube coupled to each vessel, the dip tube of each vessel being individually connected to one channel of the pump, the dip tube being fixed with respect to the vessel and positioned to permit extraction of fluid when the vessel is stopped in a specific orientation,wherein the rotor, vessels, pump, manifold and the connections to the vessels are rotatable about the horizontal axis, and fluid may be injected into or extracted from the vessels simultaneously via said one external connection under the influence of the pump.