Patent ID: 7078183
Filing Date: 2006-07-18
Classification: A23K,C12N,Y10S

Abstract:
1. A process for preparing a modified Aspergillus phytase with a specific activity improved over the specific activity of an unmodified Aspergillus phytase which comprises: (a) determining the three dimensional structure of the unmodified (b) determining from the structures of step (a) the amino acids of the active sites of the unmodified (c) constructing a DNA sequence coding for the modified phytase by obtaining the DNA sequence of the unmodified (d) integrating such a DNA sequence into a vector capable of expression in a suitable host cell; and (e) transforming the suitable host cell by the DNA sequence of step (c) or the vector of step (d), growing said host cell under suitable growth conditions and isolating the modified phytase from the host cell or the culture medium.