Patent ID: 8993297
Filing Date: 2015-03-31
Classification: C07K,C12N,C12P,C12Y

Abstract:
1. A polynucleotide encoding a chimeric glycosyltransferase, said chimeric glycosyltransferase containing a cytoplasmic tail (CT), a transmembrane domain (TMD), a stem region (SR) and a catalytic domain (CD), said polynucleotide consisting of a fusion of: 1) a nucleic acid sequence coding for the CT, said nucleic acid sequence selected from the group consisting of: SEQ ID NO:47, SEQ ID NO: 49, SEQ ID NO: 65, SEQ ID NO: 77, and SEQ ID NO: 81, said nucleic acid sequence coding for the CT being located upstream from 2) a nucleic acid sequence coding for the TMD, said nucleic acid sequence selected from the group consisting of: SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 107, SEQ ID NO: 119, and SEQ ID NO: 123, said nucleic acid coding for the TMD being located upstream from 3) a nucleic acid sequence coding for the SR, said nucleic acid sequence selected from the group consisting of: SEQ ID NO:129, SEQ ID NO: 131, SEQ ID NO: 137, SEQ ID NO: 141, and SEQ ID NO: 143, said nucleic acid sequence coding for the SR being located upstream from 4) a nucleic acid sequence coding for the CD of a native full length glycosyltransferase, said nucleic acid sequence consisting of SEQ ID NO: 43, said chimeric glycosyltransferase having at least 30-fold higher glycosylation activity towards an acceptor substrate compared to the native full-length glycosyltransferase.