Patent ID: 7922709
Filing Date: 2011-04-12
Classification: A61N

Abstract:
1. A method for delivering an isolated polynucleotide species that encodes a therapeutic gene product into skin cells of a subject, comprising: a) introducing about 0.05 μg to about 100 μg of at least one isolated polynucleotide species encoding a therapeutic gene product into each of one or more skin tissue sites of a subject; and b) substantially contemporaneously with the introduction of the isolated polynucleotide species, applying one or more electric pulses across at least two noninvasive electrodes to generate a total charge transfer of about one mC to about 1000 mC per skin tissue site so as to allow the polynucleotide species to enter cells within the skin tissue site, thereby delivering the isolated polynucleotide species into skin cells of the subject.