Patent ID: 8313652
Filing Date: 2012-11-20
Classification: B03C,Y10T

Abstract:
1. A method for separating a component of interest bound to magnetic particles from a solution comprising the following steps: (a) providing a container having one or more flat chambers wherein each flat chamber comprises an interior volume, an angular bottom ascending to an outer part of the flat chamber, a means for trapping fluids wherein the means for trapping fluid is positioned inside the outer part of the flat chamber, and a magnet positioned at an outer side of an inner part of the flat chamber for capturing the magnetic particles and the component of interest bound to the magnetic particles, (b) disposing at least a portion of the solution including the component of interest in the interior volume of the one or more flat chambers, before the container is rotating around an axis located outside the inner part of the flat chamber and adjacent to the magnet, (c) adding to the solution comprising the component of interest a multiplicity of magnetic particles, the magnetic particles being coated with a reaction component that binds to the component of interest, (d) mixing the solution with the multiplicity of magnetic particles to produce a mixture comprising magnetic particles and a supernatant liquid, and (e) separating the magnetic particles from the supernatant liquid by: spinning the mixture of magnetic particles and the supernatant liquid within the container by rotating the container such that at least one portion of the supernatant liquid is expelled to the outer part of the one or more flat chambers, wherein part or all of the supernatant liquid is trapped by the means for trapping fluids in the interior volume at the outer part of the one or more flat chambers only while the supernatant liquid is forced by centrifugal forces into the means for trapping fluid and a magnet field is applied such that the magnetic particles bind to an inner side of the inner part of the interior volume.