Patent ID: 6670126
Filing Date: 2003-12-30
Classification: C12Q

Abstract:
A process for detecting nucleic acid sequences comprising:(a) contacting a single stranded non-circular oligonucleotide primer with an amplification target circle (ATC), said ATC comprising a segment complementary to at least one segment of said primer and wherein said at least one segment includes the 3â€²-terminus of said primer, under conditions promoting hybridization of said primer with said ATC to form a primer-ATC complex, (b) contacting said complex of (a) with a rolling circle amplification (RCA)-promoting enzyme under conditions promoting rolling circle amplification of said ATC from the 3â€²-terminus of said primer, wherein said conditions include the presence of a dNTP substrate bound to a reporter molecule, to form a tandem sequence DNA (TS-DNA) that incorporates said reporter molecule, (c) contacting said TS-DNA with a plurality of primers wherein said primers are bound at their 5â€²-terminus to a reactive molecule that binds to said reporter molecule and wherein said primers comprise a segment not complementary to said TS-DNA of (b), (d) contacting said plurality of primers with a plurality of ATCs each comprising a segment complementary to a segment of said plurality of primers of (c) wherein said segment of said plurality of primers includes the 3â€²-terminus thereof, under conditions promoting rolling circle amplification of said plurality of primers to form additional TS-DNA, (e) repeating steps (c) and (d) n times where n has a value of 0 or higher, (f) detecting the presence of said TS-DNA.