Patent ID: 8921044
Filing Date: 2014-12-30
Classification: C12N,C12Q

Abstract:
1. A variant of a parent polymerase wherein (i) the parent polymerase has at least 90% sequence homology with SEQ ID NO:1 and/or SEQ ID NO:2, (ii) a difference between the parent polymerase and the variant comprises an amino acid mutation in SEQ ID NO:5 at position 5 and at least one amino acid mutation in at least one amino acid sequence selected from SEQ ID NOS: 3, 4, 6 and 8; and (iii) wherein the variant is characterized by one or more properties selected from: reducing average phasing by 1%-51% compared to the parent polymerase; increasing average sequencing read quality by 7%-75% compared to parent enzyme; increasing average sequencing read length by 1%-200% and increasing average full length sequencing reads by 25%-200%.