Patent ID: 9109195
Filing Date: 2015-08-18
Classification: C12M

Abstract:
1. A deposition apparatus which is adapted for depositing biological material in a frozen state in a target substrate, comprising a charging device, a cooling device and a driving device, wherein: (a) the charging device is adapted for providing at least one particle of the biological material at the driving device; (b) the cooling device is adapted for cooling the biological material, at least a part of the charging device and at least a part of the driving device to a temperature below −130° C; (c) the driving device is a fluid drive adapted for applying a driving force to the at least one particle of the biological material by way of a cooling gas adapted to freeze the biological material; (d) the biological material comprises at least one eukaryotic cell; (e) the target substrate comprises an isolated biological tissue, a composite composed of at least one of biological tissue and non-biological matrix material, a cell culture, a cell monolayer, a cell multilayer, a matrix material without biological cells, a synthetic cell culture device, a natural cell culture device or living biological tissue in a body of a biological organism; (f) the driving device for applying the driving force is formed in such a way that the at least one particle of the biological material can penetrate into the target substrate under an effect of the driving force without causing irreparable injury to the target substrate; and (g) there is a clear path from the charging device to the target substrate along which the at least one particle of the biological material is driven.