Patent ID: 7153654
Filing Date: 2006-12-26
Classification: C12Q,G01N

Abstract:
1. An assay procedure for detecting a target strand of DNA, said assay procedure comprising: providing a detector probe comprising a DNA sequence labeled with two chromophores, one of the two chromophores attached to the 3′ end and the second of the two chromophores attached to the 5′ end of the DNA sequence, wherein the detector probe is capable of moving between a stacked configuration that exhibits fluorescence quenching and a spaced configuration that exhibits fluorescence; adding the detector probe to a sample potentially containing a target strand of DNA, the configuration of the detector probe moving between the stacked configuration and the spaced configuration upon hybridization of the detector probe to the target strand of DNA; and detecting the presence or absence of the target strand of DNA by measuring a change in fluorescence in the sample.