Patent ID: 7968311
Filing Date: 2011-06-28
Classification: A61K,C07K,C12N,C12P

Abstract:
1. A method of producing a peptide product which comprises the steps of: (a) culturing host cells transformed or transfected with an expression vector comprising: (1) a coding region with nucleic acids coding for a peptide product coupled in reading frame 3′ of nucleic acids coding for a signal peptide and (2) a control region linked operably with the coding region, said control region comprising a plurality of promoters and at least one ribosome binding site, wherein at least one of said promoters is tac; in a culture medium in the presence of a carbon source and inducing the expression of the peptide product, while controlling growth of said host cells at a growth rate between 0.05 and 0.20 doublings per hour; and (b) thereafter recovering said peptide product from the medium.