Patent ID: 9051358
Filing Date: 2015-06-09
Classification: A61K,A61P,C07K

Abstract:
1. A method for extending half-life of bioactive peptides or proteins in vivo, comprising steps of: providing a gelatin-like protein as fusion carrier; and fusing the gelatin-like protein with the bioactive peptides or proteins to obtain a recombinant fusion protein; wherein, the gelatin-like protein has a structure characterized as follows: wherein, the Gly is glycine residues; the X and Y are selected from a group of 20 natural amino acid residues except cysteine respectively, n=20-300; wherein the gelatin-like protein is defined by: (a) a sum of Asn, Asp, Gln, Glu, Lys, Pro, Ser, Hyp and Arg residues is from 40% to ⅔ (66.7%) of the total amino acid sequence of the gelatin-like protein; (b) total number of Pro and Hyp residues is larger than 60% of the value of n; (c) total number of Gly residues is less than 1.15-fold value of n; (d) according to a ProtParam computational formula, a value of the hydrophilic index GRAVY is less than −1.5; (e) the gelatin-like protein as described excludes natural gelatin; wherein the gelatin-like protein comprises the amino acid sequence as set for the in SEQ ID NO:2 or the amino acid sequence encoded by an isolated polynucleotide molecule that has a nucleotide sequence as set forth in SEQ ID NO:3.