Patent ID: 6777208
Filing Date: 2004-08-17
Classification: C12P

Abstract:
A method for producing a 2â€²-deoxyribonucleoside, which comprises culturing a bacterium belonging to the genus Escherichia, which is transformed with a gene encoding a ribonucleotide reductase and in which the 2â€²-deoxyribonucleoside degradation activity is decreased or eliminated by disrupting a gene encoding a purine nucleoside phosphorylase on chromosomal DNA of the bacterium, in a medium in which the bacterium can grow to produce the 2â€²-deoxyribonucleoside,wherein the gene encoding a ribonucleotide reductase comprises a sequence which is amplified by PCR using oligonucleotides having sequences of SEQ ID NOs:1 and 2 as primers and the chromosoinal DNA of the bacterium as a template, and wherein the gene encoding a purine nucleoside phosphorylase comprises a sequence which is amplified by PCR using oligonucleotides having sequences of SEQ ID NOs:3 and 4 as primers and the chromosonial DNA of the bacterium as a template.