Patent ID: 6143575
Filing Date: 2000-11-07
Classification: G01N

Abstract:
A method for the heterogeneous immunochemical detection and quantitation of an analyte in a sample of a biological fluid, which method includes the following stepsa) immobilizing a first, unlabeled specific binding partner for the analyte on a pipettable, particulate solid phase,b) adding the sample to the immobilized, unlabeled specific binding partner,c) carrying out a first incubation of the reaction mixture,d) adding a defined quantity of a labeled, specific detection substance,e) carrying out a second incubation of the reaction mixture,f) precipitating the solid phase by adding at least one substance from the group which comprises the following precipitation substances:i) a second specific binding partner which is directed against the solid phase,ii) a second specific binding partner which is directed against an anchoring substance which is immobilized on the solid phase,where the second specific binding partners are preferably of a species which is different from that of the first unlabeled specific binding partner,g) centrifuging the reaction mixture,h) transferring at least some of the supernatant arising in step g) to a measuring chamber,l) starting a detection reaction in the measuring chamber, andj) determining the analyte concentration from the detection reaction.