Patent ID: 6753145
Filing Date: 2004-06-22
Classification: C12Q

Abstract:
A method of hybridizing a microarray of oligonucleotides bound to a polymer adsorbed on a surface of a siliceous substrate with a nucleic acid material comprising the step ofincubating the nucleic acid material with the microarray of oligonucleotides on the adsorbed polymer surface in a hybridization solution at a hybridization temperature ranging from about 55Â° C. to about 70Â° C. so as to hybridize the nucleic acid material, wherein the hybridization solution comprises a buffer composition that comprises a pH within a range of pH 6.4 to 7.5, a non-chelating buffering agent that maintains the pH within the pH range, and a monovalent cation in a monovalent cation concentration ranging from about 0.01 M to about 2.0 M.