Patent ID: 8067202
Filing Date: 2011-11-29
Classification: A61P,C07K

Abstract:
1. An isolated and purified superantigen toxin DNA fragment encoding toxic shock syndrome toxin-1 (TSST-1) in which amino acid position 67 is aspartate, amino acid position 70 is arginine, and amino acid position 86 is isoleucine, wherein the amino acid position numbers are based on the numbering according to the toxic shock syndrome toxin-1 amino sequence in SEQ ID NO:12, which toxic shock syndrome toxin-1 has been altered such that binding of the encoded altered toxin to either the MHC class II or T cell antigen receptor is reduced, and the toxic shock syndrome toxin-1 is not toxic to mice at levels equivalent to 10 LD 50 of the native TSST-1.