Patent ID: 6783941
Filing Date: 2004-08-31
Classification: C12N

Abstract:
A method for forming a plurality of recombined homologous double-stranded polynucleotides from at least two homologous double-stranded template polynucleotides, said method comprising the steps of:a) providing a solution comprising at least two non-methylated homologous double-stranded template polynucleotides and one or more thermostable mismatch repair protein(s); b) denaturing the template polynucleotides into single-stranded polynucleoides by increasing the temperature of the solution; c) annealing the different single-stranded polynucleotides by lowering the temperature of the solution, wherein heteroduplexes are formed; d) allowing the thermostable mismatch repair protein(s) to repair nucleotide mismatches in the heteroduplexes, wherein recombined new duplexes are formed; and e) repeating steps b) through d) at least once; wherein the new duplexes of step d) serve as new template polynucleotides in step b) in each repetition.