Patent ID: 8071342
Filing Date: 2011-12-06
Classification: A61K,B01D,C08G,C12N,C12P,F02D,Y02E

Abstract:
1. A method of producing a polyhydroxyalkanoate (PHA) and a methane monooxygenase (MMO) in a culture of methanotrophic microorganisms in the presence of non-methane organic compounds, the method comprising: a) providing a gaseous emission, wherein said gaseous emission comprises methane and one or more non-methane organic compounds; b) providing a microorganism culture medium, wherein said culture medium comprises copper, one or more of nitrogen, magnesium, phosphorus, oxygen, carbon, potassium, and/or iron, and a culture of methanotrophic microorganisms that can metabolize said methane and said one or more non-methane organic compounds; c) exposing said gaseous emission to said culture; d) adjusting the concentration of copper in said culture medium to a concentration sufficient to effect production of particulate methane monooxygenase (pMMO) or soluble methane monooxygenase (sMMO) by said culture of methanotrophic microorganisms; and e) depleting said culture medium of nitrogen, wherein said depletion causes said culture of methanotrophic microorganisms within said culture medium to generate said polyhydroxyalkanoate (PHA).