Patent ID: 6074874
Filing Date: 2000-06-13
Classification: C12N

Abstract:
A cell culture medium comprising about 2.5% to about 40% by concentration fetal bovine serum, about 2.5% to about 40% by concentration newborn calf serum, and about 1% to about 10% by concentration rat serum, such that the total concentration of sera is no greater than about 45%, wherein (A) said fetal bovine serum and said newborn calf serum are selected by pretesting the effects of the sera on the growth and differentiation of cultured embryonic stem cells in the presence of LIF, such that sera that cause spontaneous differentiation of said cultured embryonic stem cells are rejected, and (B) epithelial cells cultured in said cell culture medium form secondary structures which morphologically resemble those in the organs from which said epithelial cells are obtained.