Patent ID: 6475733
Filing Date: 2002-11-05
Classification: C12N,C12Q,G01N

Abstract:
A method for identifying a plurality of mutated receptors that bind a ligand to produce an identifying fingerprint for the ligand, comprising:generating a plurality of nucleic acids that encode a plurality of mutated receptors, introducing the plurality of nucleic acids into a plurality of cells, there being different nucleic acids in different of the plurality of cells, expressing the plurality of nucleic acids in the plurality of cells to produce a plurality of mutated receptors on the surface of the cells, contacting the plurality of cells with the ligand, detecting an intracellular signal in a cell generated by a ligand binding to one of the plurality of mutated receptors, the signal being indicative of the presence of a mutated receptor that binds the ligand, wherein the mutated receptor is selected from the group consisting of mutated G-protein coupled receptors, tyrosine kinase receptors and ion channels, and selecting a plurality of mutated receptors based upon the generated intracellular signals to produce an identifying fingerprint for the ligand.