Patent ID: 8124373
Filing Date: 2012-02-28
Classification: C12N,C12P,C13K

Abstract:
1. A method of producing a biologically active fragment of a polypeptide, wherein the method comprising: producing a fragment of a polypeptide that comprises an amino acid sequence selected from the group consisting of the amino acid sequence set forth in SEQ ID NO: 8 or 10, and an amino acid sequence that is encoded by a first nucleotide sequence that hybridizes to a second nucleotide sequence encoding SEQ ID NO: 8 or 10 under high stringency conditions, wherein the conditions comprise hybridization at 65° C. in 1% BSA, 1 mM EDTA, 0.5M NaHPO (a) catalyses the conversion of sucrose into isomaltulose and concomitantly produces trehalulose at a yield of less than 5% of the yield of isomaltulose; or (b) catalyses the conversion of sucrose into isomaltulose with a K (c) catalyses the conversion of sucrose into isomaltulose but not the hydrolysis of isomaltulose; and detecting sucrose isomerase activity associated with the fragment, which indicates that the fragment is a biologically active fragment.