Patent ID: 8911973
Filing Date: 2014-12-16
Classification: C12Q

Abstract:
1. A composition, selected from the group consisting of: I) a first composition, configured for generating a 3′ hairpin structure from a target nucleic acid, said first composition comprising a snap-back primer and a reverse primer, wherein said snap-back primer comprises: wherein said reverse primer comprises a 3′ region configured to hybridize to said first amplification product such that said reverse primer can be extended by a polymerase to generate a second amplification product comprising a 3′ snap-back portion capable of hybridizing to a non-adjacent portion of said second amplification product to form a 3′ hairpin structure, wherein said 3′ hairpin structure comprises a 3′ terminal portion configured to not hybridize to said second amplification product; and II) a second composition, configured for generating a 5′ hairpin structure from a target nucleic acid, said second composition comprising a snap-back primer, wherein said snap-back primer comprises: wherein said amplification product comprises a 5′ snap-back portion capable of hybridizing to a non-adjacent portion of said amplification product to form a 5′ hairpin structure, wherein said 5′ hairpin structure comprises a 5′ terminal portion not hybridized to said amplification product.