Patent ID: 7575868
Filing Date: 2009-08-18
Classification: C12Q

Abstract:
1. A method for assessing chemotherapeutic efficacy for a cancer patient, comprising: allowing a multicellular tumor tissue explant of the patient to form a cell culture monolayer, removing the explant from the culture when the monolayer is at about 10 to about 50 percent confluency and growing the monolayer, thereby preventing substantial growth of stromal cells; optionally preparing a subculture from the monolayer cells; conducting at least one phenotypic assay on cells from the monolayer or the subculture, so as to determine a tumor cell phenotype predictive of a drug's efficacy against the tumor; conducting at least one genotypic analysis of cells from the patient to determine the presence or absence of a genotype predictive of resistance to said drug; thereby assessing whether the drug will be an effective chemotherapy for the patient.