Patent ID: 8658360
Filing Date: 2014-02-25
Classification: C12N,C12Q

Abstract:
1. A method of generating a purified RNA and purified DNA sample from an initial sample that comprises DNA and RNA molecules, said method comprising; a) contacting said initial sample with; i) a dilution buffer with an acidic pH, and ii) a nucleic acid binding matrix, which comprises a zeolite, that preferentially binds both single and double stranded DNA molecules in the presence of said dilution buffer, wherein said contacting generates a DNA-bound binding matrix which is substantially RNA free; and b) physically separating said DNA-bound binding matrix from said initial sample the remaining initial sample now being a purified RNA sample comprising a plurality of RNA molecules; c) contacting the DNA-bound binding matrix with an elution buffer; and d) eluting DNA bound to the DNA-bound binding matrix thereby generating a purified DNA sample comprising a plurality of DNA molecules.