Patent ID: 7429460
Filing Date: 2008-09-30
Classification: A61K,C12Q,G01N

Abstract:
1. A method of screening for a compound that inhibits deleterious effects due to glucosylceramide (GlcCer) activated phosphatidylcholine (PC) accumulation related to Gaucher disease, the method comprising the steps of: a. providing a cell culture in which the cells are overexpressing GlcCer; b. exposing the cell culture to at least one candidate compound; and either c. measuring the synthesis of PC in the cell culture, wherein a significant decrease in the synthesis of PC as compared to a cell culture incubated without the candidate compound identifies said candidate compound as an inhibitor of PC accumulation; or d. measuring the degradation of PC in the cell culture, wherein a significant increase in the degradation of PC as compared to a cell culture incubated without the candidate compound identifies said candidate compound as an inhibitor of PC accumulation.