Patent ID: 6852909
Filing Date: 2005-02-08
Classification: C12N

Abstract:
1. A process for producing trangenic plants containing selectively destructable pistil- or stigma parts, wherein the plant possesses resistance to PCT as a result of phosphinothricir fixed precisely by the sequence. The protein sequence is defined by the DNA sequence, An ATG codon, which is recognized in bacteria and plants, is used as the translation start codon; the Shine-Dalgarno sequence is underlined. This gene encodes the last step in the biosynthesis of PTT, i.e. the deacetylation of inactive N-acetylphosphinothricin tripeptide to give the active PTT, It is known that the specificity of many enzymes is not restricted to one substrate. Thus, the phosphinothricin N-acetyltransferase which is encoded by the pat gene is actually used in PTT biosynthesis for acetylating desmethyl-PTC and, because of its lack of specificity, can be used for detoxifying PTC. By means of overexpressing the dea gene (using suitable promoters or by cloning onto high-copy vectors), an insufficiently specific N-acetyl-PTT deacetylase can now be employed for activating N-acetylphosphinothricin, Other dea genes can be isolated from acetyltransferease activity, in addition, is given an N-Ac-PTC or N-Ac-PTT deacetylase gene under the control of a pistil- or stigma specific promoter, and the pistil- or stigma are caused to die by means of suitable, timely treatment with PTC or PTT.