Patent ID: 7943346
Filing Date: 2011-05-17
Classification: C07K,C12Q

Abstract:
1. A method of simultaneously assaying for the presence and identity of a plurality of target bacterial species selected from the group consisting of Klebsiella pneumoniae, Pseudomonas aeuroginosa, Proteus mirabilis, Streptococcus pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Staphylococcus saprophyticus, Streptococcus pyogenes, Haemophilus influenzae , and Moraxella catarrhalis , and at least one target bacterial antibiotic resistance gene selected from the group consisting of bla tem , bla rob , bla shv , aadB, aacC1, aacC2, aacC3, aacA4, mecA, vanA, vanH, vanX, satA, aacA-aphD, vat, vga, msrA, sul, and int, by performing an assay comprising: simultaneously contacting a sample with a set of amplification primers comprising a plurality of primer pairs, said plurality of primer pairs comprising: amplifying target nucleic acids from the sample under the single amplification protocol; and determining the presence or amount of target nucleic acids from the first, second and third target bacterial species, wherein at least one target nucleic acid is selected from the group consisting of: SEQ ID NO. 37 and a complementary sequence thereof, for determining the presence or amount of