Patent ID: 8822217
Filing Date: 2014-09-02
Classification: C12N

Abstract:
1. A method for cultivating tendon cells from non-embryonic pluripotent cells of mesenchymal origin, the method comprising the steps of: cultivating isolated non-embryonic pluripotent cells of mesenchymal origin in a culture medium under standard culture conditions in a culture vessel; before a complete confluence of the cultivated non-embryonic pluripotent cells of mesenchymal origin, further cultivating the cultivated non-embryonic pluripotent cells of mesenchymal origin in a culture medium mixed with ascorbic acid and/or ascorbic acid-2-phosphate in a concentration of 25 to 75 μg/ml; and before a complete confluence of the cultivated non-embryonic pluripotent cells of mesenchymal origin, subjecting the cultivated non-embryonic pluripotent cells of mesenchymal origin to hyperosmolar treatment in a culture medium whose osmolarity is adjusted to 350 to 500 mosmol/l using chemically and biologically inert osmotic substances.