Patent ID: 9121061
Filing Date: 2015-09-01
Classification: C12N,C12Q,C12Y

Abstract:
1. A method for differentiating unmethylated cytosine (C) and 5-methylcytosine (5-mC) from 5-hydroxymethylcytosine (5-hmC), 5-formylcytosine (5-fC), 5-carboxycytosine (5-CaC) or 5-glycosylated hydroxymethylcytosine (5-ghmC), comprising: (a) reacting a polynucleotide optionally containing C, 5-mC, 5-hmC, 5-fC, 5-CaC and/or 5-ghmC, with a cytidine deaminase having an amino acid sequence that is at least 90% identical to SEQ ID NO:2, wherein 5-mC is converted to a thymine (T) and C is converted to a uracil (U); and (b) amplifying or cleaving the polynucleotide to identify the location of at least one converted nucleotide in the polynucleotide.