Patent ID: 7960525
Filing Date: 2011-06-14
Classification: A61K,A61P,C12N

Abstract:
1. A method of attenuating expression of an endogenous gene of interest in a cell in vitro, said method comprising: a) introducing into the cell a vector comprising targeting DNA, wherein said targeting DNA is flanked by a restriction endonuclease site and comprises (1) DNA homologous to a targeting site of the endogenous gene of interest and (2) DNA which attenuates expression of the gene of interest by recombination between said targeting DNA and the gene of interest; and b) introducing into the cell a restriction endonuclease which cleaves the restriction endonuclease site, thereby increasing the probability of recombination between said targeting DNA and the gene of interest, and thereby increasing the probability of obtaining a cell with the expression of the endogenous gene of interest being attenuated.