Patent ID: 9207690
Filing Date: 2015-12-08
Classification: B01L,C12P,G05D

Abstract:
1. A method, comprising: obtaining, with a controller of a thermal control system, a target sample temperature, from a computer memory of the thermal control system that stores the target sample temperature, for a sample for a first sub-step, of a plurality of predetermined sub-steps, of a processing step, of a plurality of predetermined processing steps, to be performed on the sample, wherein the first sub-step of the processing step is one of a denaturing, an annealing or an extending of a replication of a polymerase chain reaction sub-step; obtaining, with the controller of the thermal control system, a temperature map, from the computer memory of the thermal control system that stores the temperature map, wherein the temperature map provides a mapping between the target sample temperature, as measured by a thermocouple in thermal communication with a channel of a calibration carrier with the target sample temperature applied to the channel, and a plurality of different temperatures for a thermal control device, wherein the plurality of different temperatures are different from each other and different from the target sample temperature and correspond to different time points, of a predetermined set of time points, of the first sub-step; identifying, with the controller of the thermal control system, for a first time point of the different time points for the first sub-step, a first temperature of the plurality of different temperatures using the mapping between the target sample temperature and the plurality of different temperatures and the first time point; and controlling, with the controller of the thermal control system, a temperature of the thermal control device at the first time point of the first sub-step to be the first temperature, wherein the thermal control device is in thermal communication with a channel of a sample carrier carrying the sample and controls a temperature of the sample.