Patent ID: 6441160
Filing Date: 2002-08-27
Classification: B01J,C12N

Abstract:
A method for separating a plasmid from a solution containing the plasmid together with contaminants selected from a group consisting of proteins, RNAs, DNAs and mixtures thereof, using hydrophobic interaction chromatography, comprising the steps of:a) selecting a first column material for a first hydrophobic interaction chromatography that does not adsorb the plasmid at a salt concentration at which protein and RNA are adsorbed, b) selecting a second column material for a second hydrophobic interaction chromatography that adsorbs the plasmid at a salt concentration at which the first column material adsorbs protein and RNA and the first column material does not adsorb the plasmid, c) injecting the solution containing the plasmid and contaminants into a first column containing the first column material to adsorb protein and RNA onto the first column material and form an eluate containing the plasmid and DNA, d) injecting the eluate containing the plasmid and DNA from the first column into a second column containing the second column material to adsorb the plasmid and DNA onto the second column material, and e) eluting the adsorbed plasmid from the second column material with an eluent containing salt of a concentration at which the plasmid is eluted from the second column material.