Patent ID: 7422882
Filing Date: 2008-09-09
Classification: C12N

Abstract:
1. A thermostable DNA polymerase having a 3′-5′ exonuclease activity and the amino acid sequence of a thermostable DNA polymerase of Pyrococcus furiosus, Pyrococcus kodakaraensis KOD 1 or Thermococcus litoralis , with a single modification, wherein the modification is a replacement of histidine (H) in the DIETLYH (SEQ ID NO:35) or DIETFYH (SEQ ID NO:36) sequence (D: aspartic acid, I: isoleucine, E: glutamic acid, T: threonine, L: leucine, F: phenylalanine, Y: tyrosine, H: histidine) within the exonuclease I region by another amino acid.