Patent ID: 9134228
Filing Date: 2015-09-15
Classification: G01J,G01N

Abstract:
1. A method for determining the concentration of a polysorbate species in a mixture, comprising: a) obtaining a test sample of said mixture; b) adding an internal standard to said test sample; c) obtaining, with a spectrometer, a mid-infrared Attenuated Total Reflectance (ATR) spectra of the test sample; d) identifying, from the reflectance spectra, a wavenumber corresponding to the C═O peak; e) identifying, from the reflectance spectra, a wavenumber corresponding to the internal standard; f) calculating the area under the curve for said C═O wavenumber, normalized by the internal standard; and g) comparing said area to a calibration curve to determine the concentration of polysorbate in said test sample.