Patent ID: 8354252
Filing Date: 2013-01-15
Classification: C07H,C12Q

Abstract:
1. A method of monitoring a nucleic acid synthesis reaction, comprising: contacting a polymerase/template/primer complex with a fluorescently labeled nucleotide or nucleotide analog having a nucleotide or nucleotide analog component, a fluorescent label component, and a linker component joining the nucleotide or nucleotide analog component to the label component, wherein the linker component is coupled to the nucleotide or nucleotide analog component through a 5′- phosphate group on the nucleotide or nucleotide analog component, and the linker component maintains the label component at a functional distance away from the nucleotide or nucleotide analog component of at least 2 nm; and detecting, a characteristic signal from the fluorescent dye indicative of incorporation of the nucleotide or nucleotide analog into a primer extension reaction.