Patent ID: 6489104
Filing Date: 2002-12-03
Classification: A61K,C07K

Abstract:
A method of screening for a DNA encoding a protein that regulates the human p27Kip1 promoter activity of a promoter DNA, said promoter DNA comprising at least a substantial portion of a fragment extending from nucleotide 2795 to nucleotide 3133 of the nucleotide sequence of SEQ ID NO:1, wherein said promoter DNA possesses promoter activity and retains the ability to promote the expression of the human p27Kip1 gene, said method comprising a step selected from the group consisting of:(a) bringing a test protein encoded by a test DNA into contact with said promoter DNA and selecting the test DNA encoding the test protein that binds to said promoter DNA; (b) introducing a test DNA encoding a test protein into cells carrying a reporter gene fused downstream of said promoter DNA, testing for the expression of said reporter gene, and selecting the test DNA encoding the test protein that regulates the expression of said reporter gene; and (c) bringing a test protein encoded by a test DNA into contact with cells carrying a reporter gene fused downstream of said promoter DNA, testing for the expression of said reporter gene, and selecting the test DNA encoding the test protein that regulates the expression of said reporter gene; wherein the test DNA selected in step (a), (b), or (c) represents a DNA encoding a protein that regulates the human p27Kip1 promoter activity of the promoter DNA.