Patent ID: 6383807
Filing Date: 2002-05-07
Classification: A61K,C12N

Abstract:
A method of transforming hematopoietic cells which comprise T lymphocytes, said method comprising the steps of:(a) providing viral particles comprising a foreign nucleic acid sequence which comprises: (i) a regulatory nucleic acid sequence which is expressable in T lymphocytes and which can be activated by a signal supplied to said T lymphocytes by an antigen that induces graft versus host disease, and (ii) a nucleic acid sequence that encodes a product which, when contacted in situ with a pharmaceutical substance, reacts with said substance to induce destruction of said T lymphocytes, wherein said nucleic acid sequence is operably linked to said regulatory nucleic acid sequence; and (b) culturing said viral particles, under conditions suitable for cell culturing, with said T lymphocytes, thereby transforming said T lymphocytes with said foreign nucleic acid sequence.