Patent ID: 8825411
Filing Date: 2014-09-02
Classification: C12N,C12Q,G16B

Abstract:
1. A method of designing a polynucleotide, the method comprising: a) selecting an initial polynucleotide sequence that codes for a polypeptide, wherein a codon frequency in said initial polynucleotide sequence is determined by a codon bias table; and b) modifying an initial codon choice in said initial polynucleotide sequence in accordance with a design criterion, thereby constructing a final polynucleotide sequence that codes for said polypeptide; wherein the design criterion comprises: minimized sequence identity to a reference polynucleotide, and wherein modifying said initial codon choice in said initial polynucleotide sequence in accordance with said design criterion comprises altering a plurality of codons in said initial polynucleotide sequence to minimize sequence identity to said reference polynucleotide; and synthesizing said final polynucleotide sequence.