Patent ID: 7709001
Filing Date: 2010-05-04
Classification: A61K,A61P,C07K

Abstract:
1. A method for making an immunogenic conjugate comprising Streptococcus pneumoniae serotype 3 polysaccharide covalently linked to a carrier protein, the method comprising: (a) reacting purified serotype 3 polysaccharide with a mild acid resulting in a hydrolyzed serotype 3 polysaccharide; (b) reacting the hydrolyzed serotype 3 polysaccharide with an oxidizing agent in the presence of bivalent cations resulting in an activated serotype 3 polysaccharide; (c) compounding the activated serotype 3 polysaccharide with a carrier protein; (d) reacting the compounded, activated serotype 3 polysaccharide and carrier protein with a reducing agent resulting in a serotype 3 polysaccharide:carrier protein conjugate; and (e) capping unreacted aldehydes in the serotype 3 polysaccharide:carrier protein conjugate resulting in an immunogenic conjugate comprising