Patent ID: 8841438
Filing Date: 2014-09-23
Classification: C12N,G01N

Abstract:
1. An RNA construct comprising a non-naturally occurring, continuous sequence of ribonucleotide bases defining: a stem-loop structure; simulated, three way junction joining regions 3′ and 5′ of the stem-loop structure; a first region 5′ of the 5′ joining region comprising bases complementary to a 3′ region of a target polynucleotide, wherein the target polynucleotide is not continuous with the RNA construct; a second region 3′ of the 3′ joining region comprising bases complementary to a 5′ region of the target polynucleotide; the base sequence of the first and second regions being selected to base pair with complementary bases on the target polynucleotide spaced apart by an intermediate region on the target polynucleotide defining another three way junction joining region, whereby,