Patent ID: 9074262
Filing Date: 2015-07-07
Classification: C12Q,Y10S

Abstract:
1. A method for amplifying HIV-1 nucleic acid in a sample, comprising: a) contacting the sample with a pair of oligonucleotide primers that bind to a first primer binding site and a second primer binding site located within the LTR region of the HIV-1 genome; and b) performing a nucleic acid amplification under conditions wherein said oligonucleotide primers bind only to said first and second primer binding sites, thereby amplifying HIV-1 nucleic acid in the sample; wherein said pair of oligonucleotide primers consists of a first primer and a second primer, wherein said first primer consists essentially of a first oligonucleotide that is fully complementary to a sequence of the LTR region at the first primer binding site, said oligonucleotide being 15-26 nucleotides in length and comprising at least 15 sequential nucleotides of the nucleotide sequence of: said first oligonucleotide being operably linked to a promoter; and wherein said second primer consists essentially of a second oligonucleotide that is fully complementary to a sequence which is the reverse complement of a sequence of the LTR region at the second primer binding site, said oligonucleotide being 10-26 nucleotides in length and comprising at least 10 sequential nucleotides of the nucleotide sequence of: