Patent ID: 9116354
Filing Date: 2015-08-25
Classification: G01N,G02B

Abstract:
1. A STED-SPIM-microscope comprising: a light source sending an excitation light beam from a y-direction onto an object to be imaged; a camera detecting in a z-direction as a first detection direction light emanating from the object as at least one of fluorescent light and reflected light, wherein the z-direction extends substantially perpendicular to the y-direction; a first x-scanner generating a sequential light sheet by scanning the excitation light beam in an x-direction, wherein the x-direction extends substantially perpendicular to the y-direction and to the z-direction and the light sheet is sequentially formed in a plane that is defined by the x-direction and the y-direction; a deactivation light source sending from the y-direction via the first x-scanner that is a joint scanner for the excitation light beam and deactivation light beam at least one deactivation light beam that is scanned in x-direction onto the object making the effective sequentially generated light sheet that is generated by scanning the excitation light beam in the x-direction thinner in the z-direction, wherein the deactivation light beam has an intensity maximum that is at least offset in the z-direction in relation to the excitation light beam; and a deactivation light beam modulator having a structure that modulates the deactivation light beam to a cross-sectional shape that comprises in z-direction at least two intensity maxima with a zero point in between that is provided in the center of the excitation light beam.