Patent ID: 8822226
Filing Date: 2014-09-02
Classification: G01N,Y10T

Abstract:
1. A method for quick and simultaneous determination of 16 inorganic anions and organic acids in tobacco by ion chromatography, wherein the elution process comprises: analysing filtrates extracted from a tobacco sample in accordance with the following elution process and conditions; conditions of said ion chromatograghy being: anion exchange column: IonPacAS11-HC (2 mm×50 mm) and IonPacAG11-HC (2 mm×250 mm); column temperature: 30° C.; flow rate: 0.30 ml/min; injection volume: 25 μL; suppressor current: 50 mA; and wherein the method further comprises steps for pre-treating the tobacco sample before analysis, the steps for pre-treating the tobacco sample consisting of: (1) determining the percentage of moisture in the tobacco sample; (2) pre-treating the tobacco sample by extracting the tobacco sample ultrasonically by extraultra-purified water at a ratio of 1 g tobacco sample: 10 mL water and then filtering the sample to collect the filtrates; and (3) diluting the filtrates tenfold and then filtering the filtrates.