Patent ID: 8652833
Filing Date: 2014-02-18
Classification: C12M

Abstract:
1. A method of producing a cell culture chamber having a concave-convex pattern formed on a surface on which cells are cultured, the method comprising the steps of: forming cell culture portions, micro flow channels communicating with the cell culture portions, and an introduction area communicating with the cell culture portions through the micro flow channels, the cell culture portions, the micro flow channels, and the introduction area being each formed of a concave portion of the concave-convex pattern; dropping a liquid containing a cell adhesion-inducing substance on the introduction area so as to introduce the liquid only to the concave portion of the micro flow channels and subsequently to the cell culture portions by capillarity; and drying the liquid introduced to the concave portion so as to coat the cell adhesion-inducing substance to the concave portion of the microflow channels and cell culture portions; wherein said micro flow channels have a width ranging from 1 μm to 500 μm and a depth ranging from 2 μm to 500 μm; and wherein the cell culture portions have a width ranging from 5 μm to 1,000 μm.