Patent ID: 6069000
Filing Date: 2000-05-30
Classification: A23L,C12G,C12N,C12Y

Abstract:
DNA construct comprising a DNA sequence encoding an enzyme exhibiting pectin esterase activity, wherein said sequence is selected from the group consisting of:(a) a pectin esterase-encoding part of the DNA sequence cloned into plasmid pYES 2.0 present in E. coli DSM 10357;(b) a DNA sequence corresponding to nucleotides 4-933 of SEQ ID NO: 1; and(c) a DNA sequence whichi) is at least 70% homologous with the DNA sequence defined in (a) or (b), wherein said homology is determined using GAP, with a GAP creation penalty of 5.0 and a GAP extension penalty of 0.3; orii) hybridizes with the DNA sequence shown in positions 4-933 in SEQ ID NO 1 at medium stringency, oriii) encodes a polypeptide which is at least 60% homologous with the polypeptide encoded by a DNA sequence comprising the DNA sequence defined in (a) or (b), wherein said homology is determined using GAP, with a GAP creation penalty of 3.0 and a GAP extension penalty of 0.1.