Patent ID: 8263354
Filing Date: 2012-09-11
Classification: G01N

Abstract:
1. A method of selecting a patient for treatment of a cancer with 10-propargyl-10-deazaaminopterin, the method comprising the steps of: (a) obtaining a sample of the patient's cancer tissue; (b) determining the expression level of a polypeptide comprising a member of a folate pathway polypeptide within a cell comprising least one of reduced folate carrier-1 enzyme (RFC-1), dihydrofolate reductase (DHFR), folylpoly-gamma-glutamate synthetase (FPGS), thymidylate synthase (TS), γ-glutamyl hydrolase (GGH), and glycinamide ribonucleotide formyltransferase (GARFT) in the sample; (c) comparing the determined expression level in the sample with a reference expression level for the polypeptide comprising a member of a folate pathway polypeptide within a cell; and (d) selecting the patient for treatment 10-propargyl-10-deazaaminopterin where the comparison of the expression level in the sample of the polypeptide comprising a member of a folate pathway polypeptide within a cell and the corresponding reference expression level indicate sensitivity of patient's cancer tissue to 10-propargyl-10-deazaaminopterin.