Patent ID: 9138743
Filing Date: 2015-09-22
Classification: B01L

Abstract:
1. An assay device for detection of an analyte in a fluidic sample, the device comprising: (a) a microfluidic chamber comprising a single fluidic channel having a first inlet, an outlet, and an axis; (b) a first surface in communication with the first inlet and the outlet, wherein the first surface is disposed within the single fluidic channel and wherein the first surface comprises a plurality of capture regions, wherein the capture regions are upstream of the outlet; (c) a plurality of capture agents immobilized on the first surface within the capture regions, wherein the capture agents specifically bind the analyte; (d) a reagent storage depot in communication via the single fluidic channel with the first surface, wherein the storage depot is disposed within the single fluidic channel and wherein the storage depot comprises a plurality of reagent regions aligned with corresponding capture regions; (e) a plurality of detection reagents that specifically bind the analyte and that become mobile upon contact with fluid, wherein the detection reagents are disposed within the reagent regions, and wherein fluid traverses from the plurality of reagent regions to corresponding capture regions in parallel with the alignment of the reagent regions to the corresponding capture regions.