Patent ID: 7629152
Filing Date: 2009-12-08
Classification: C12Q

Abstract:
1. A method for amplifying a nucleotide sequence of interest in a nucleic acid polymer, which method comprises: (a) contacting the nucleic acid polymer with a primer set comprising a plurality of non-replicable primers and a plurality of replicable primers, to form a reaction mixture in which, (b) subjecting the reaction mixture to conditions such that (i) the non-replicable primer hybridizes to the nucleic acid polymer and forms the first primer extension product, and (ii) the replicable primer hybridizes to the first primer extension product to form the second primer extension product; (c) separating the first and second primer extension products from the nucleic acid polymer templates and treating the resulting mixture with the primer set under conditions such that additional first and second primer extension products are produced; and (d) repeating step (c) to provide a reaction mixture wherein the at least a portion of the nucleotide sequence of interest or its complement is amplified polynomially.