Patent ID: 8092997
Filing Date: 2012-01-10
Classification: C07H,C12Q

Abstract:
1. A method for synthesizing a nucleic acid molecule using a dual specificity oligonucleotide by a template-dependent extension reaction, comprising: (a) annealing the dual specificity oligonucleotide to a template nucleic acid molecule, wherein the dual specificity oligonucleotide is represented by the formula: 5′-X wherein the T wherein the separation portion has the lowest T wherein the 5′-high T wherein the separation portion forms a non base-pairing that does not serve as a hybridizing site and does not interact with the template nucleic acid molecule for base pairing under conditions that the 5′-high T wherein the annealing is performed under conditions that annealing solely by the 3′-low T wherein 5′-high T (b) extending the dual specificity oligonucleotide to synthesize the nucleic acid molecule complementary to the template nucleic acid.