Patent ID: 6187590
Filing Date: 2001-02-13
Classification: G01N,Y10T

Abstract:
A method of producing a white blood cell (""WBC"") fraction of a hematology control solution such that the WBC fraction retains its light scatter capabilities, the method comprising:a. providing mammalian whole blood or buffy coat layer;b. combining the whole blood or buffy coat layer with a lysing reagent for from about 1 to 5 minutes in a ratio of from about 1 part blood or buffy coat layer to about 12 parts lysing reagent to eliminate any red blood cells present and to form a cell suspension, wherein the lysing reagent is present in an amount from about 1 part lyse to about 12 parts diluent;c. combining the cell suspension of step b with a fixative to form a mixture and incubating the mixture at from about 60.degree. C. to about 70.degree. C. for up to about 10 minutes to fix and stabilize the cells;d. cooling the mixture of step c to ambient temperature and allowing to sediment;e. discarding a supernatant formed in step d and washing the resultant fixed cells sufficient to remove the active reagents of steps b and c; andf. resuspending the fixed and washed cells in a resuspension medium suitable for long term storage of the fixed cells, wherein the fixed cells exhibit multi-angle light scattering characteristics similar to the multi-angle light scattering characteristics of WBC of interest so that subpopulations of WBC may be differentiated with the control solution solely by the use of a multi-angle light scatter hematology analyzer.