Patent ID: 7381405
Filing Date: 2008-06-03
Classification: A61K,C12N

Abstract:
1. A method of preparing autologous T-lymphocytes for re-introduction into a patient having cancer, which method comprises: (i) obtaining peripheral blood mononuclear cells (PBMCs) from a patient immunized with an antigen of the cancer, (ii) stimulating the PBMCs with the antigen of the cancer in vitro, and (iii) transducing the PBMCs with a retroviral vector, which (a) comprises and expresses a human interleukin-2 (IL-2) coding sequence operably linked to a retroviral promoter, (b) does not comprise an exogenously introduced gene that enables phenotypic selection, and (c) comprises a viral envelope that efficiently transduces CD8+ T-lymphocytes, whereupon autologous T-lymphocytes are prepared for re-introduction into a patient having cancer.