Patent ID: 6319716
Filing Date: 2001-11-20
Classification: A61K,A61P,C12N

Abstract:
A method for constructing a recombinant BAV3 vector containing a heterologous sequence inserted into an insertion site, the method comprising:(a) linking the heterologous sequence to sequences which are substantially homologous to BAV3 sequences surrounding the insertion site, to form an insertion cassette; wherein said insertion site is located in a region of the BAV3 genome consisting of nucleotides 4092 through 5234; nucleotides 5892 through 17,735; nucleotides 21,198 through 26,033; or nucleotides 31,133 through 34,445 of FIGS. 20:1-20:19 (SEQ ID NO: 35);(b) introducing the insertion cassette into a cell, along with a polynucleotide comprising a sequence that is substantially homologous to a BAV3 genome; and(c) allowing homologous recombination to occur between the insertion cassette and the polynucleotide to generate the recombinant BAV3 vector.