Patent ID: 6355453
Filing Date: 2002-03-12
Classification: C12P

Abstract:
An enzymatic synthesis of a fluorinated sugar having at least 8 carbon atoms, said synthesis comprising reacting a Fâ€”CH2â€”COâ€”COOâˆ’M+ with a furanoside or pyranoside sugar substrate in the presence of an aldolase enzyme, wherein M+ is H+ or a pharmaceutically acceptable cation, and wherein the concentration of aldolase enzyme, per 50 mM of Fâ€”CH2â€”COâ€”COOâˆ’M+, is 1 to 2,500 U*/ml, where U* represents an enzyme activity unit and is the enzyme activity with respect to the standard reaction of pyruvate and N-acetylmannosamine to obtain the N-acetyl derivative of neuraminic acid, further comprising the step of recovering the fluorinated sugar.