Patent ID: 6331421
Filing Date: 2001-12-18
Classification: C07K,C12N

Abstract:
A DNA construct comprising an expression vector, wherein said expression vector comprises in downstream order of transcription:(1) a promoter;(2) a ribosomal binding site;(3) an initiation codon and a DNA spacer sequence positioned between the ribosomal binding site and initiation codon;(4) a human cytoplasmic Cu/Zn superoxide dismutase structural gene in reading frame with said initiation codon having a stop codon at the 3'-terminus of said genes,wherein said DNA spacer sequence, said initiation codon, and the first two codons of said human cytoplasmic Cu/Zn superoxide dismutase structural gene comprise the sequence:X.sub.n X.sub.n X.sub.n X.sub.n ATGGCXACXwherein each n is independently 1, and each X is independently A, C, T, or G; and(5) a transcription terminator,wherein said DNA construct, upon introduction into E. coli, causes the expression of active human cytoplasmic Cu/Zn superoxide dismutase.