Patent ID: 8703734
Filing Date: 2014-04-22
Classification: C12Q,G01N,Y10T

Abstract:
1. A method of detecting a target biomolecule, comprising: contacting a sample with a probe for a target biomolecule, wherein the probe is attached to a solid substrate, and wherein the probe comprises: allowing the functional groups to specifically interact with the target biomolecule; applying laser light that can excite the donor fluorophore to enter the solid substrate; trapping the laser light in the solid substrate by total internal reflection; exciting the donor fluorophore, wherein when interaction of the functional groups in the presence of the target brings the acceptor fluorophore into a proximity with a donor fluorophore to permit excitation of the acceptor fluorophore by the donor fluorophore and results in the production of a signal by the acceptor fluorophore; and detecting the signal from the acceptor fluorophore when the probe interacts with target biomolecule.