Patent ID: 6790614
Filing Date: 2004-09-14
Classification: C07K,C12N

Abstract:
A method for identifying transduced mammalian hematopoietic cells comprising:a) retrovirally transducing mammalian hematopoietic cells with a nucleic acid sequence encoding a mutated epidermal growth factor receptor (EGFR) operatively linked to an expression control sequence, wherein said mutated EGFR comprises modifications to the intracellular and the extracellular domains, comprises a modification to the extracellular domain, or comprises a modification to the intracellular domain; b) incubating the transduced mammalian hematopoietic cells with a marked antibody which recognizes and binds specifically to the mutated EGFR; and c) identifying the marked transduced mammalian hematopoietic cells.