Patent ID: 8658389
Filing Date: 2014-02-25
Classification: A61K,C07K,C12N,G01N

Abstract:
1. A method for detecting a molecule of interest in a tissue section or a cytology sample, comprising: contacting the tissue section or cytology sample with an antibody-polyethylene glycol (PEG) linker-signal-generating moiety conjugate comprising an antibody covalently linked to at least 3 signal-generating moieties, wherein each signal-generating moiety is linked to the antibody through a monodisperse, heterobifunctional PEG linker to provide an antibody-PEG linker-signal-generating moiety conjugate having the formula wherein Ab is an antibody, SM is an enzyme signal-generating moiety, n=4 to 12 and s=3 to 10; and detecting a signal generated by the antibody-PEG linker-signal-generating moiety conjugate that indicates the presence of the molecule of interest in the sample.