Patent ID: 7067322
Filing Date: 2006-06-27
Classification: B01J,B01L,B82Y,C40B,G01N,Y10T

Abstract:
1. A method of immobilizing biomolecules or cells to a metal substrate comprising: (a) depositing an amino-C (b) contacting the ω-modified monolayer of step (a) with a heterobifunctional compound comprising a first moiety reactive with the ω-modified alkanethiol monolayer and a second moiety comprising a protected thiol moiety, under conditions wherein the heterobifunctional compound binds to the ω-modified alkanethiol monolayer via the first moiety; (c) deprotecting the second moiety of the heterobifunctional compound to yield an unprotected thiol moiety; and then (d) attaching a biomolecule or cell to the unprotected thiol moiety of step (c).