Patent ID: 7322810
Filing Date: 2008-01-29
Classification: C12N,C12Q,G01N

Abstract:
1. A method for identifying a Myc activation inhibitor compound comprising the steps of measuring the ability of a test compound to interfere with the activity of the E3-ligase HectH9, which has the amino acid sequence of SEQ ID NO: 2, wherein HectH9 activity is measured by: a) the transfer of ubiquitin from a ubiquitin conjugating enzyme E2 to HectH9, or to a fragment thereof that has E3-ligase activity, or b) the transfer of ubiquitin from HectH9 to Myc or a C-terminal fragment thereof that contains a critical site for ubiquitination by HectH9, wherein said measurement is conducted after a period of time sufficient to allow for the ubiquitination reaction to occur; and wherein a decrease in the level of said HectH9 activity in the presence of a test compound, as compared to the level of said HectH9 activity in the absence of said test compound, is indicative of said compound's potential to inhibit the activation of Myc.