Patent ID: 6936696
Filing Date: 2005-08-30
Classification: C07K,C12N

Abstract:
1. A modified polypeptide comprising a substitution of 1 or more essential amino acids selected from the group consisting of isoleucine and tryptophan into an unmodified polypeptide with the amino acid sequence of SEQ ID NO: 1; wherein said tryptophan is substituted into said unmodified polypeptide in place of at least one amino acid selected from the group consisting of N32, Y35, N40, N50, Y72, H88, Y116, H119, Y132, Y133, P137, Y158, Y172, E200, P239, Y249, P265, P324, Y330, Y346, N368, and Y411; and wherein said isoleucine is substituted into said unmodified polypeptide in place of at least one amino acid selected from the group consisting of N32, L36, F46, G51, L56, F59, Q65, L66, Y72, R73, V75, Q76, L85, H88, F94, L95, L96, F97, V98, L99, R102, L105, L107, N117, L118, D122, Q124, R125, P 127, Y132, Y133, L134, V135, P137, L143, K147, L148, P151, Y158, F162, Q169, L173, L181, V209, P239, F240, P247, F256, E258, N264, P265, L267, F273, L274, L285, L286, H288, N290, V295, L297, V298, N300, L308, V309, K317, K319, P324, L334, V339, F340, V341, Y346, F348, V350, L356, F358, L359, F361, N368, F372, F393, Y411, F412, and V413, wherein said modified polypeptide accumulates in a seed.