Patent ID: 6077708
Filing Date: 2000-06-20
Classification: G01N,Y10T

Abstract:
A method of determining the content of progenitor cells in a hematopoietic cell culture, the method comprising:culturing a reference culture of hematopoietic cells under selected conditions, the selected conditions comprising employing a selected feeding protocol;measuring one or more metabolic parameters selected from the group consisting of glucose consumption, lactate production and oxygen consumption of the reference culture at a plurality of selected times;determining the content of progenitor cells in the reference culture at each of the selected times;culturing an experimental culture of hematopoietic cells under essentially the same conditions used to culture the reference culture;measuring the same one or more metabolic parameters for the experimental culture at one or more selected time(s) as measured for the reference culture; andcomparing the one or more metabolic parameters measured for the experimental culture with those measured for the reference culture to determine the content of progenitor cells in the experimental culture at the selected time(s).