Patent ID: 6975943
Filing Date: 2005-12-13
Classification: C12N,C12Q

Abstract:
1. A method for determining a sequence of a target nucleic acid, comprising: a) providing a plurality of clones containing fragments of the target nucleic acid in an array comprising predetermined axes, whereby the position of each clone in the array may be identified; b) pooling multiple clones of a first axis and preparing a first library from the pooled clones; c) performing random sequencing reads on the pooled clones of b), thereby generating sequence coverage of the pooled clones; d) pooling multiple clones of a second axis and preparing a second library from the pooled clones, wherein the second axis intersects the first axis at a nexus, and wherein the nexus comprises at least one clone that is common to the first and second axis; e) performing random sequence reads on the pooled clones of d) thereby generating sequence coverage of the pooled clones; f) cross-assembling the random sequence reads of c) with the random sequence reads of e), thereby generating a sequence contig associated with the nucleic acid present in the nexus clone; g) repeating steps a) through f) to generate multiple contigs; and h) combining contigs of step g) and determining the sequence of the nucleic acid present in the pooled clones.