Patent ID: 8697443
Filing Date: 2014-04-15
Classification: C12M,C12N

Abstract:
1. A method of culturing cells in a cell culture device comprised at least in part of a gas permeable material and including at least one access port and including at least two scaffolds, the method comprising: a) adding cells and a volume of liquid medium into said cell culture device; b) orienting said cell culture device into an inoculation position such that said scaffolds reside at different elevations within said cell culture device; c) allowing cells to settle upon said scaffolds; d) adding enough liquid medium to prevent a unique gas-liquid interface from forming directly above at least one scaffold when the device is oriented in the inoculation position and to have at least a portion of the liquid medium in contact with at least a portion of said gas permeable material; e) placing the cell culture device in a cell culture location that includes ambient gas at a composition suitable for cell culture, said ambient gas making contact with said gas permeable material; and f) not perfusing said liquid medium when said device is in said cell culture location.