Patent ID: 6368812
Filing Date: 2002-04-09
Classification: A01K,A61K,C07K,G01N

Abstract:
A process for determining whether a chemical compound is a galanin receptor (GALR3) agonist which comprises contacting cells transfected with DNA or infected with RNA encoding and expressing on their cell surface the human or rat galanin receptor (GALR3) or a membrane fraction from such cells, with the compound under conditions permitting the activation of the galanin receptor (GALR3), and detecting an increase in galanin receptor (GALR3) activity, so as to thereby determine whether the compound is a galanin receptor (GALR3) agonist, wherein such cells prior to being transfected with such DNA or injected with RNA do not express the galanin receptor (GALR3), and wherein the human galanin receptor (GALR3) has an amino acid sequence identical to the amino acid sequence shown in FIG. 4 (SEQ ID NO: 4) or that encoded by plasmid pEXJ-hGalR3 (ATCC Accession No. 97827); and the rat galanin receptor (GALR3) has an amino acid sequence identical to the amino acid sequence shown in FIG. 2 (SEQ ID NO: 2) or that encoded by plasmid PEXJ-RGalR3T (ATCC Accession No. 97826) or plasmid K1086 (ATCC Accession No. 97747).