Patent ID: 6933129
Filing Date: 2005-08-23
Classification: A61P,G01N

Abstract:
1. A method for preparing a biopsy sample of tissue containing malignant cells for chemosensitivity testing, comprising: (a) obtaining a tumor specimen containing malignant cells and unwanted cells, wherein said malignant cells are susceptible of overgrowth of said unwanted cells; (b) mechanically separating said specimen into cohesive multicellular particulates having a size of about 0.25-1.25 mm3; (c) growing a tissue culture monolayer from said cohesive multicellular particulates without overgrowth of said unwanted cells; (d) inoculating malignant cells from said monolayer into a plurality of segregated sites; (e) treating said plurality of sites with at least one agent; (f) examining said plurality of sites; and (g) assessing chemosensitivity of the cells in said plurality of sites; and (h) monitoring culture medium in which said monolayer is grown for production of soluble secreted factors indicative of a disease state or lack thereof.