Patent ID: 6265181
Filing Date: 2001-07-24
Classification: C12Q

Abstract:
A method for determining the mutant prevention concentration (MPC) of a drug against a particular pathogen, the method comprising:obtaining a culture of said pathogen grown to high density;applying at least 10.sup.10 colony-, plaque- or foci-forming units of said pathogen to a plurality of groups of containers, each container housing solid growth medium, wherein each group of containers comprises a particular concentration of said drug, and wherein the concentrations of said drug differ from one another by at least about two-fold;incubating said containers;counting the pathogen colonies, plaques, or foci in said containers;plotting the number of counted colonies, plaques, or foci for each group of said containers representing a given drug concentration against the drug concentration in at least some of the groups of said containers; and,extrapolating or interpolating the plot to determine the minimum drug concentration corresponding to zero colonies, plaques, or foci, said minimum drug concentration being the MPC.