Patent ID: 6479052
Filing Date: 2002-11-12
Classification: A61K,A61L,A61P,C12N

Abstract:
A method of adhering keratinocytes to a target surface which is a wound of a mammal coated with a biodegradable polymer sheet comprising:harvesting keratinocytes from cell culture prior to their reaching confluence; adhering a biodegradable polymer sheet to the tissue surface that is the target surface; coating the biodegradable polymer sheet at the target surface with a mixture of a first component comprising a non-polymeric fibrin-related protein and a second component effective for converting the fibrin-related protein to fibrin polymer; and spraying a suspension of the keratinocytes onto the fibrin polymer-coated target surface, wherein the mixed two components have formed a fibrin polymer with a tack effective to adhere the keratinocytes, wherein a colonization promoting effective amount of the cells is secured on the target surface and the fibrin polymer forms in an amount effective to secure a colonization promoting effective amount of the cells on the target surface, and wherein the application of fibrin polymer and fibrin-polymer adhered keratinocytes is effective to provide the formation of epidermal cover at the target surface.