Patent ID: 7189508
Filing Date: 2007-03-13
Classification: C12Q

Abstract:
1. A method of generating a signal indicative of the presence of a target nucleic acid sequence in a sample, comprising forming a detection complex by incubating a sample comprising a target nucleic acid sequence, and a probe, wherein said probe comprises a first and a second subunit, and a pair of interactive signal generating moieties effectively positioned on said probe to quench the generation of a detectable signal when the probe is not bound to said target nucleic acid or when said first subunit of the probe is associated with said second subunit of the probe, and wherein said first or second subunit of said probe comprises said pair of interactive signal generating moieties, and wherein said first subunit of said probe comprises a binding moiety, and binding said probe to said target nucleic acid sequence wherein said first subunit of said probe dissociates from said second subunit of said probe to generate said signal, wherein said binding is performed at a binding temperature and said first subunit of said probe does not dissociate from said second subunit of said probe when not bound to said target nucleic acid sequence at or below said binding temperature; and wherein generation of said signal is indicative of the presence of a target nucleic acid sequence in said sample.