Patent ID: 8969003
Filing Date: 2015-03-03
Classification: C07H,C12Q

Abstract:
1. A method for the preparation of mismatched nucleic acid duplexes comprising: a) providing a mixture including a sample containing one or more target nucleic acids and at least one oligonucleotide that is substantially complementary to the target nucleic acid, wherein said oligonucleotide comprises at least one 3-alkynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one analogue, and forms a duplex with target nucleic acid of substantially the same stability, regardless which natural nucleic acid base is positioned opposite to the 3-alkynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one base; and b) incubating the mixture under hybridization conditions, wherein the 3-alkynyl-1H-pyrazolo[3,4-d]pyrimidin-4(5H)-one analogue is substituted with pyrene or acridine.