Patent ID: 7927601
Filing Date: 2011-04-19
Classification: A61K,C07K,C12N,C12Q,G01N

Abstract:
1. An isolated Hepatitis B virus (HBV) variant or genome thereof comprising nucleotide mutations in a nucleic acid sequence encoding a reverse transcriptase (rt) portion of an HBV DNA polymerase, which result in amino acid substitutions in said reverse transcriptase from: leucine (L) to methionine (M) at amino acid 180 (rtL180M); alanine (A) to threonine (T) at amino acid 194 (rtA194T); and methionine (M) to valine (V) at amino acid 204 (rt M204V); wherein the foregoing amino acid substitutions are numbered with reference to M of the YMDD motif in HBV DNA polymerase being amino acid number 204, and wherein said variant exhibits decreased sensitivity to tenofovir disoproxil fumarate (TDF).