Patent ID: 7955844
Filing Date: 2011-06-07
Classification: A01K,C07K,C12N

Abstract:
1. A noninvasive method for detecting the level of expression of a gene of interest in an animal using multimodality imaging, comprising: (a) administering a triple fusion vector to the animal, wherein the triple fusion nucleic acid expression vector comprises a first reporter nucleic acid sequence, a second reporter nucleic acid sequence, a third reporter nucleic acid sequence, a first nucleic acid linker sequence, and a second nucleic acid linker sequence, wherein: (b) administering coelenterazine to the animal; (c) immobilizing the animal within the detection field of a photodetection device; (d) measuring the level of bioluminescent light emission in the animal with the photodetection device; (e) measuring the level of fluorescent light emission in the animal with the photodetection device; and (f) immobilizing the animal within the detection field of a PET device, and measuring the level of positron emission.