Patent ID: 9108194
Filing Date: 2015-08-18
Classification: B01L,C12N,C12Q,G01N,Y10T

Abstract:
1. A processing device for fragmenting nucleic acids contained within a sample, the device comprising: a first chamber for receiving the sample containing nucleic acids; a first cap attached to the first chamber; a channel in fluid communication with the first chamber, the channel having a volume; a shearing orifice in fluid communication with the channel and adapted to fragment the nucleic acids in the sample upon flow of the sample through the shearing orifice, wherein the first chamber and the channel are constructed and arranged to maintain a relatively constant pressure at an entrance of the shearing orifice during flow of a majority of a sample through the shearing orifice, the sample having a sample volume that is at least 2 times the channel volume; a second chamber in fluid communication with the shearing orifice and for receiving the sample containing the fragmented nucleic acids from the shearing orifice, wherein the first chamber, the channel, the shearing orifice and the second chamber are constructed and arranged for use with a flow actuator to move the sample containing nucleic acids from the first chamber through the channel and the shearing orifice into the second chamber, wherein only one shearing orifice is located between the first chamber and the second chamber; and a second cap attached to the second chamber.