Patent ID: 6773707
Filing Date: 2004-08-10
Classification: A61K,C07K,C12N,Y10S

Abstract:
A method for inducing an immune response to a tumor antigen comprising administering to a subject an immunologically effective amount of a complex of;(a) a hybrid antigen comprising an antigenic domain of the tumor antigen and a binding domain that noncovalently binds to a eukaryotic hsp70, and comprises a heptameric region having the sequence motif HyXHyXHyXHy (SEQ ID NO:29) where Hy represents a hydrophobic amino acid and X is any ammo acid, wherein the antigenic domain and the binding domain are separated by a short peptide linker; and (b) the eukaryotic hsp70; wherein the hsp70 is from the same species as the subject; wherein the antigenic domain is from a first source and the binding domain is from a second source different from the first source; wherein the binding domain, the short peptide linker, and fee antigenic domain are covalently bound in vitro; and wherein the hybrid antigen and the hsp70 are combined in vitro under conditions wherein binding of the hybrid antigen to the hsp70 occurs to form a non-covalent hybrid antigen-hsp70 complex.