Patent ID: 8192987
Filing Date: 2012-06-05
Classification: A61K,A61P,C12N,G01N

Abstract:
1. A method for obtaining an isolated dental follicle stem cell said method comprising the steps of taking cells from tissue surrounding the epithelial enamel organ of a human tooth (dental follicle), culturing the taken cells, and isolating a plastic adherent cell from the cell culture, wherein the plastic adherent cell (i) self-renews, (ii) differentiates into cells of endodermal, mesodermal, or ectodermal lineage, (iii) expresses markers nestin, notch-I, and vimentin, but is devoid of markers CD34 and CD133, (iv) differentiates in vitro in the presence of dexamethasone into a tissue-like structure comprising calcium deposits, and (v) builds a calcium-containing follicle-tissue membrane when stimulated with dexamethasone in vitro.