Patent ID: 8980604
Filing Date: 2015-03-17
Classification: C12N,C12P

Abstract:
1. A mutant bacterial glycerol dehydrogenase (GlyDH) enzyme, wherein unmutated GlyDH enzyme from E.coli comprises at least one amino acid sequence selected from the group consisting of: (a) an amino acid sequence consisting of a Conserved Region 1 (CR1): -Pro-Thr-X1-X2-X3-X4-X5-X6-X7-X8-X9-(CR1) Wherein X1 is Ile, X2 is Ala, X3 is Ser, X4 is Thr, X5 is Asp, X6 is Ala, X7 is Pro, X8 is Cys, and X9 is Ser; and (b) an amino acid sequence consisting of a Conserved Region 2 (CR2): -X10-X11-X12-X13-X14-X15-X16-X17-X18-X19-X20-Gly-X21-X22-Asp (CR2) wherein X10 is Val, X11 is Ala, X12 is Gly, X13 is Ala, X14 is Pro, X15 is Ala, X16 is Arg, X17 is Leu, X18 is Leu, X19 is Ala, X20 is Ala, X21 is Ile, and X22 is Gly, wherein said unmutated GlyDH enzyme comprises an amino acid substitution at amino acid X4 in CR1 and/or an amino acid substitution at amino acid X13 in CR2, and wherein inhibition of said mutant GlyDH enzyme activity by NAD