Patent ID: 8647864
Filing Date: 2014-02-11
Classification: A61K,C07K,C12N

Abstract:
1. A eukaryotic layered vector initiation system, comprising: (a) a 5′ promoter capable of initiating in vivo the 5′ synthesis of alphavirus RNA from cDNA, (b) a sequence following the 5′ promoter which initiates transcription of alphavirus RNA, (c) a nucleic acid molecule which operably encodes all four alphaviral nonstructural proteins, (d) an alphavirus RNA polymerase recognition site, and (e) a 3′ polyadenylate tract, wherein the nucleic acid sequence which operably encodes all four alphaviral nonstructural proteins contains a mutation in at least non-structural protein 2 (nsp2), wherein the mutation is in one or more residues selected from the group consisting of residue 438, residue 622, and residue 634, numbered according to wild-type Sindbis nsp2 encoded by a nucleic acid molecule of SEQ ID NO: 1.