Patent ID: 6235892
Filing Date: 2001-05-22
Classification: C12N

Abstract:
A process for the purification of nucleic acids comprising the following steps:(a) directing an aqueous feed solution consisting essentially of a buffered solution containing at least one nucleic acid and at least one endotoxin through at least one microporous weakly basic anion exchange membrane so as to bind at least a portion of both said nucleic acid and said endotoxin to said membrane; and(b) directing an elution solution consisting essentially of a buffered neutral salt solution of 2M NaCl at pH 5.5 through said membrane so as to selectively elute from said membrane at least a major portion of said nucleic acid bound to said membrane in step (a)wherein the buffer used in steps (a) and (b) comprises an aqueous solution of tris-HCl and EDTA at pH 8.0 wherein the concentration ratio of tris-HCl to EDTA is 10 to 1.