Patent ID: 8198044
Filing Date: 2012-06-12
Classification: C07K,C12N,C12P

Abstract:
1. A translation system for the expression of a polypeptide of interest comprising at least one unnatural amino acid at a specified position, the system comprising: (a) an unnatural amino acid; (b) a nucleic acid construct, said construct comprising a nucleotide sequence encoding an orthogonal tRNA (O-tRNA) and a nucleotide sequence encoding an orthogonal aminoacyl-tRNA synthetase (O-tRNA), wherein said O-tRNA preferentially aminoacylates said O-tRNA with said unnatural amino acid, wherein said nucleic acid construct comprises at least one of: (c) a polynucleotide encoding said polypeptide of interest, said polynucleotide comprising at least one selector codon that is recognized by said O-tRNA, wherein the position of the selector codon in the polynucleotide controls the specified position of the unnatural amino acid in the polypeptide of interest upon expression of the polynucleotide to produce the polypeptide.