Patent ID: 6649649
Filing Date: 2003-11-18
Classification: A61K

Abstract:
A method of treating colonic adenomas in a patient in need thereof comprising administering to said patient a non-toxic therapeutically effective amount of an NSAID, with the proviso that said NSAID is not aspirin, ibuprofen, indomethacin, sulindac, dolobid, diclofenac, naproxen, piroxicam, etodolac, ketoprofen, flurbiprofen, meloxicam, flosulide or nabumetone,wherein said NSAID is a potent inhibitor of PGHS-2 and wherein said NSAID possesses a selectivity for inhibiting PGHS-2 over PGHS-1 of at least 100 fold as measured by the ratio of the IC50 for the inhibition of PGHS-2 to the IC50 for the inhibition of PGHS-1 in a whole cell or microsomal assay, said assay conducted by measuring the amount of prostaglandin E2 synthesized in the presence of arachidonic acid, PGHS-2 or PGHS-1 enzyme and said NSAID, and wherein said assay comprises the steps of: (1) incubating said NSAID with the cell or microsome expressing PGHS-2 or PGHS-1 enzyme for about 10 to 20 minutes prior to the addition of arachidonic acid, and (2) conducting said assay with arachidonic acid at a concentration of about 10 &mgr;M.