Patent ID: 6358737
Filing Date: 2002-03-19
Classification: A01K,C07K,C12N

Abstract:
A method for the production of an osteocyte cell line, said cell line having the following properties: available to passage more than 20 times, a stellate shape with dendritic processes and expresses increased levels of osteocalcin compared to mouse osteoblast or osteoblast-like cell lines, and said method comprising the steps of:(a) isolating bones from a transgenic mouse whose genome contains an osteocalcin promoter driven T-antigen transgene and preparing bone pieces from the bones; (b) digesting the bone pieces with collagenase solution and harvesting cells into fetal and adult calf serum supplemented medium; and (c) plating the harvested cells and isolating said cell line by selecting a single colony.