Patent ID: 7009180
Filing Date: 2006-03-07
Classification: A61B,G01N

Abstract:
1. A method of measuring concentration of glucose in a fluid sample comprising, in no particular order: providing a cuvette; providing a source of electromagnetic radiation that emits radiation through the cuvette; providing a detector that detects radiation emitted from the source, the detection occurring after the radiation has traveled a pathlength, traveling a pathlength comprising passing at least through the cuvette; inserting saline reference fluid into the cuvette; using the saline reference fluid to determine the pathlength; using the pathlength to determine the correct reference spectrum for sample water absorbance, and calculating the correct reference spectrum for sample water absorbance or selecting the correct reference spectrum for sample water absorbance from among a plurality of stored reference spectra; using the pathlength to determine the correct reference spectrum for sample protein absorbance, and calculating the correct reference spectrum for sample protein absorbance or selecting the correct reference spectrum for sample protein absorbance from among a plurality of stored reference spectra; inserting a fluid sample into the cuvette; emitting a range of wavelengths from the source through the cuvette and the fluid sample; obtaining total sample absorbance data by measuring, through the range of wavelengths, the amount of radiation that reaches the detector; subtracting the correct reference water and protein absorbance spectra from the total sample absorbance data; and using the remaining absorbance spectrum, after the correct reference water and protein absorbance spectra have been subtracted, to determine concentration of glucose in the fluid sample.