Patent ID: 8541345
Filing Date: 2013-09-24
Classification: C12N,C12Q,C40B,G01N

Abstract:
1. A composition comprising an S. cerevisiae strain SK1 yeast library, wherein each member of the yeast library has histone H3 and histone H4 deleted from its genome; and wherein the wild-type histone sequence of one of said two histones H3 and H4 is expressed from a plasmid; and wherein the remaining one of said two wild-type histone sequences that is not expressed from a plasmid is substituted with a mutant histone sequence expressed from a plasmid; and wherein said mutant histone sequence has one of its wild-type amino acid residues substituted with alanine; and wherein each member of said yeast library has only a single mutation; and wherein each amino acid position mutation in H3 and H4 is present at least one time in said library.