Patent ID: 6706486
Filing Date: 2004-03-16
Classification: G01N,Y10S,Y10T

Abstract:
A method of releasing thermostable immunoligands bound to the Fc-part of immunoglobulins in immunoglobulin-immunoligand complexes and preventing rebinding thereof, and assaying said thermostable immunoligands comprising:prediluting a sample containing said immunoglobulin-immunoligand complexes, with a buffered solution having a defined protein content; mixing said prediluted sample with a reagent compound that binds non-specifically or adsorbs to said immunoglobulins; heating said prediluted sample mixed with reagent compound thereby releasing said thermostable immunoligands from said Fc-part; thereafter, cooling said prediluted sample mixed with reagent compound; and carrying out an immunochemical assay on said prediluted sample mixed with reagent compound to detect, or detect and quantify said thermostable immunoligands, wherein said prediluted sample mixed with reagent compound is further combined with sample dilution buffer used in the assay, said sample dilution buffer having a solution of said reagent compound added thereto, in a concentration that prevents rebinding of said thermostable immunoligands to said Fc-part.