Patent ID: 7001746
Filing Date: 2006-02-21
Classification: A61L,C12N

Abstract:
1. A method of identifying a protein, a polypeptide or a peptide secreted from a differentiated human adipocyte, said method comprising: a) isolating human preadipocytes and culturing said preadipocytes in a preadipocyte medium for a period of time, wherein said preadipocytes are plated at a density of about 25,000 to 40,000 cells/cm b) differentiating said human preadipocytes with a differentiation medium comprising a defined cell culture medium; wherein said medium comprises 1.0-4.5 g/liter glucose, a cyclic AMP inducer, a glucocorticoid or a glucocorticoid analogue, insulin or an insulin analogue, and a Peroxisome Proliferator Activated Receptor gamma agonist or a retinoic acid X receptor agonist effective to stimulate differentiation of said human preadipocytes; c) fractionating the conditioned medium of the differentiated adipocytes; d) comparing the pattern of proteins, polypeptides or peptides in the conditioned medium of said differentiated adipocytes with the pattern of proteins, polypeptides or peptides secreted by an undifferentiated adipocyte; e) identifying the protein, polypeptide or peptide secreted by the differentiated adipocyte.