Patent ID: 8309318
Filing Date: 2012-11-13
Classification: G01N

Abstract:
1. A method of producing a combined signal correlating with a presence of both antigen-specific immunoglobulins and C3d complement fragments in a biological sample comprising: (a) exposing a solid support comprising an immobilized antigen to the biological sample, wherein said antigen-specific immunoglobulins present in the biological sample are specific to and bind to said immobilized antigen; (b) washing unbound molecules from the solid support; (c) exposing said solid support to at least two binding partners: (i) first labeled binding partners comprising first signal-generating labels, wherein the first labeled binding partners specifically bind the antigen-specific immunoglobulins bound to the immobilized antigen; and (ii) second labeled binding partners comprising second signal-generating labels, wherein the second labeled binding partners specifically bind the C3d complement fragments bound to the antigen-specific immunoglobulins bound to the immobilized antigen; (d) washing unbound first and second labeled binding partners from the solid support; and (e) producing a combined absorbance value from the first signal-generating labels of the first labeled binding partners bound to the solid support and the second signal-generating labels of the second labeled binding partners bound to the solid support; wherein the first signal-generating labels and the second signal-generating labels are the same, and wherein said combined absorbance value correlates to the presence of antigen-specific immunoglobulins and C3d complement fragments in the biological sample.