Patent ID: 8932839
Filing Date: 2015-01-13
Classification: C12N,C12P,C12Y,Y02P

Abstract:
1. A method of production of isopulegol of general formula (I) comprising cyclizing citronellal of general formula (II) to isopulegol of formula (I) by means of an enzyme mutant with cyclase activity which is a mutant of a wild-type enzyme comprising the amino acid sequence of SEQ ID NO: 2 with a mutation at a position corresponding to position F486 of the amino acid sequence of SEQ ID NO: 2, wherein up to 10% of the amino acid residues in said enzyme mutant are altered relative to SEQ ID NO: 2 by deletion, insertion, substitution, addition, inversion, or a combination thereof, and wherein said enzyme mutant catalyzes at least the cyclization of a citronellal isomer to at least one isopulegol isomer, or in the presence of a microorganism expressing said enzyme mutant.