Patent ID: 9113623
Filing Date: 2015-08-25
Classification: A01N,C12N,C12Q

Abstract:
1. A method of producing a cell viability reagent for a tissue sample, the method comprising: providing at least one chaotrope; providing a first and a second kosmotrope, wherein the first kosmotrope is glycerol and the second kosmotrope is α,α-trehalose; providing a chelator; providing a buffer; providing an apoptosis reducing substrate, wherein the apoptosis reducing substrate is Leptin; providing a metabolic modulator; mixing the at least one chaotrope, the first and second kosmotrope, the chelator, the buffer, the apoptosis reducing substrate, and the metabolic modulator to produce the cell viability reagent wherein the cell viability reagent is capable of preserving the tissue sample for gene expression analysis, wherein the tissue sample is a surgically excised cancer tissue sample, and wherein a final concentration of the chaotrope is from about 0.1 Molar to about 2 Molar, a final concentration of the first and the second kosmotrope is from about 0.1 Molar to about 2 Molar, a final concentration of the chelator is from about 0.1 Molar to about 2 Molar, and a final concentration of the apoptosis reducing substrate is from about 0.001 Molar to about 0.5 Molar.