Patent ID: 6831161
Filing Date: 2004-12-14
Classification: C07K,C12N,C40B

Abstract:
A method of affinity separation, comprising the steps: of:(a) providing a sample containing a target analyte; (b) providing a matrix comprising an immobilized protein, said immobilized protein comprising one or more modifications that (i) increase the stability of said protein in alkaline conditions, and (ii) permit said protein to bind to said target analyte, said one or more modifications selected from the group consisting of: (1) deleting one or more Asn residues in said protein; (2) substituting one or more Asn residues in said protein for an amino acid that is less sensitive to alkaline conditions; (3) chemically modifying one or more Asn residues in said protein; and (4) combinations thereof; (c) contacting said sample and said matrix, wherein said target analyte binds to said immobilized protein; and (d) isolating said target analyte from said matrix.