Patent ID: 6300086
Filing Date: 2001-10-09
Classification: A61K,A61P,C07K

Abstract:
A method of identifying an inhibitor of IL-1-R intracellular domain binding which comprises:(a) transforming a cell with a first polynucleotide encoding an IL-1-R intracellular domain protein, a second polynucleotide encoding an IL-1-R intracellular ligand protein, and at least one reporter gene, wherein the expression of the reporter gene is regulated by the binding of the IL-1-R intracellular ligand protein encoded by the second polynucleotide to the IL-1-R intracellular domain protein encoded by the first polynucleotide;(b) growing the cell in the presence of and in the absence of a compound; and(c) comparing the degree of expression of the reporter gene in the presence of and in the absence of the compound;wherein the compound inhibits IL-1-R intracellular domain binding when a decrease in the degree of expression of the reporter gene occurs, and wherein said second polynucleotide is selected from the group consisting of:(1) a polynucleotide comprising the nucleotide sequence of SEQ ID NO:1 from nucleotide 2 to nucleotide 529 and(2) a polynucleotide encoding an IL-1-R intracellular ligand protein comprising the amino acid sequence of SEQ ID NO:2.