Patent ID: 7563567
Filing Date: 2009-07-21
Classification: C12Q

Abstract:
1. A high-throughput method for assessing genomic CpG methylation and expression of genomic sequences of a tissue sample, comprising: obtaining a microarray, or replicates thereof, having a plurality of affixed CpG-rich genomic probe fragments each comprising a first exon sequence or a detectably hybridizable portion of the first exon sequence of an expressible gene; and hybridizing, to the microarray or replicate thereof, CpG-rich genomic DNA-derived target sequences of a tissue sample, and mRNA-derived target sequences having exon 1 sequences of the respective expressible genes of the tissue sample, wherein the DNA-derived target sequences and the mRNA-derived target sequences are detectibly labeled, wherein the extent of said target hybridizations with each such represented expressible gene probe is reflective of the presence of methylated genomic CpG sequences and genomic expression, respectively, in the tissue sample, and whereby both genomic CpG methylation and genomic expression of particular expressible genes represented on the microarray, or replicate thereof, are, at least in part, assessed.