Patent ID: 6348314
Filing Date: 2002-02-19
Classification: C12N,C12Q,Y10S

Abstract:
A method for detecting the presence of a target nucleic acid molecule by detecting non-target cleavage products comprising:a) providing: i) a cleavage agent; ii) a source of target nucleic acid, said target nucleic acid comprising a first region and a second region, said second region downstream of and contiguous to said first region; iii) a first oligonucleotide, wherein at least a portion of said first oligonucleotide is completely complementary to said first portion of said first target nucleic acid; iv) a second oligonucleotide comprising a 3â€² portion and a 5â€² portion, wherein said 5â€² portion is completely complementary to said second portion of said target nucleic acid; b) mixing said cleavage agent, said target nucleic acid, said first oligonucleotide and said second oligonucleotide to create a reaction mixture under reaction conditions such that at least said portion of said first oligonucleotide is annealed to said first region of said target nucleic acid and wherein at least said 5â€² portion of said second oligonucleotide is annealed to said second region of said target nucleic acid so as to create a cleavage structure, and wherein cleavage of said cleavage structure occurs to generate non-target cleavage product; and c) detecting the cleavage of said cleavage structure.