Patent ID: 6399386
Filing Date: 2002-06-04
Classification: A61K,C07K,C12N

Abstract:
A method for identifying a product of interest comprising:a) providing a cDNA expression library from an organism of interest, the inserts e.g. being under the control of an inducible promoter; b) providing a population of yeast cells that express (e.g. constitutively) a DNA sequence; c) transforming the population of yeast cells with the cDNA expression library; and d) detecting protein kinase activity in clonally-derived yeast cells as an indication of the presence of DNA encoding the product of interest, wherein the product of interest is: (I) a ligand for a tyrosine kinase receptor, and the DNA sequence is a copy of a gene encodinig a heterologous tyrosine kinase receptor, or (II) a heterologous tyrosine kinase receptor which is activated by binding to a known polypeptide ligand and the DNA sequence encodes the known polypeptide ligand, or (III) a ligand for a transmembrane receptor and the DNA sequence encodes a fusion protein comprising the extracellular domain of the transmembrane receptor fused to the intracellular domain of a tyrosine kinase receptor, or (IV) a ligand for a cytokine receptor and the DNA sequence encodes a fusion protein comprising the extracellular domain of the cytokine receptor fused to the intracellular domain of a tyrosine kinase receptor, or (V) a cytokine receptor that is activated by binding to a known polypeptide ligand and the DNA sequence encodes a polypeptide ligand and a cytoplasmic tyrosine kinase.