Patent ID: 8357509
Filing Date: 2013-01-22
Classification: A01N,C07K,C12P,Y02A

Abstract:
1. A method for producing a recombinant tobacco osmotin polypeptide, the method comprising: (a) growing in culture media microbial host cells transformed with a vector that inducibly expresses the tobacco osmotin polypeptide, wherein the culture media comprises about 2-20 μM Fe (b) inducing expression of the tobacco osmotin polypeptide; (c) adjusting pH of the culture media to about 6.5-7.5 after inducing expression of the tobacco osmotin polypeptide; and (d) isolating the tobacco osmotin polypeptide, wherein at least about 50 mg tobacco osmotin polypeptide per liter of culture media is isolated.