Patent ID: 7632509
Filing Date: 2009-12-15
Classification: C07K,C12N

Abstract:
1. A method for producing a recombinant immunoglobulin or a recombinant immunoglobulin fragment, comprising the steps of: a) transducing a host cell with a lentivector, said lentivector comprising: in the 5′ to 3′ direction, a promoter operably linked to an open reading frame transcriptional unit comprising (1) a sequence encoding a heavy chain of an immunoglobulin or a fragment of the heavy chain, (2) a sequence encoding a furin cleavage site, (3) a sequence encoding a 2A self-processing cleavage site, wherein the 2A self-processing cleavage site is the FMDV 2A oligopeptide SEQ ID NO: 9, and (4) a sequence encoding a light chain of an immunoglobulin or a fragment of the light chain; b) expressing the recombinant immunoglobulin or the recombinant immunoglobulin fragment in said transduced host cell, wherein said sequence encoding the heavy chain of the immunoglobulin or the fragment of the heavy chain and said sequence encoding the light chain of the immunoglobulin or the fragment of the light chain are expressed from the same host cell.