Patent ID: 6288044
Filing Date: 2001-09-11
Classification: A61K,A61P,A61Q

Abstract:
Process for preparation of O-sulfated K4, defructosylated K4, K5 and K40 polysaccharides comprising the following steps:a) suspension of K4, defructosylated K4, K5 or K40 polysaccharide in the form of a sodium salt in an aprotic solvent;b) O-sulfation with a pyridine-sulphur trioxide or trimethylamine-sulphur trioxide adduct or with chlorosulfonic acid;c) dilution with water or with 0.2-1 N NaCl;d) pH adjustment to a basic value;e) precipitation by addition of ethanol saturated with sodium acetate or methanol. isopropanol or acetone;f) dissolution by a NaCl solution;g) diafiltration;h) precipitation of the product by ethanol;i) drying.