Patent ID: 8747294
Filing Date: 2014-06-10
Classification: A61B,C12N,G01N

Abstract:
1. A method for predicting embryonic progression of a preimplantation embryo, comprising the steps of: generating a preimplantation embryo by fertilizing an oocyte and subsequently incubating the fertilized oocyte in vitro in an insemination medium, thereby generating the preimplantation embryo; measuring levels of nitric oxide metabolites in the insemination medium (IM) in which the oocyte was fertilized and subsequently incubated in vitro at 18-30 hours post fertilization; and determining that the preimplantation embryo will progress to a blastocyst stage by culture day 5 based on whether the levels of nitric oxide metabolites in the insemination medium are at least about 1.3 uM.