Patent ID: 8999653
Filing Date: 2015-04-07
Classification: G01N,Y10T

Abstract:
1. A method for detecting the internalization of a transmembrane protein of interest expressed at the surface of a cell which is contained in a reaction medium, comprising: a) labeling, in said reaction medium, the protein of interest with a fluorescent metal complex comprising a lanthanide or ruthenium, the lifetime of which is greater than 0.1 ms; b) adding to said reaction medium a compound capable of causing the internalization of the protein of interest; c) adding to said reaction medium a modulating agent which is a fluorescent FRET acceptor compound compatible with said fluorescent metal complex, wherein said the acceptor is not conjugated to a compound which can bring said acceptor into proximity with said fluorescent metal complex, and wherein the acceptor freely diffuses in the reaction medium, the final concentration of which in the reaction medium is greater than 10 d) measuring the signal emitted by said reaction medium at the emission wavelength of the fluorescent metal complex and/or at the emission wavelength of the modulating agent when said modulating agent is a fluorescent acceptor compound; e) comparing the signal measured in step d) with a reference signal measured on cells having been subjected only to steps a), c) and d), a difference in the signal measured in step d) compared with the reference signal being representative of the internalization of the protein of interest.