Patent ID: 6100040
Filing Date: 2000-08-08
Classification: C07H,C12Q

Abstract:
A method for genetic testing, comprising:a) obtaining isolated unlabeled nucleic acid sequences from a first organism suspected of containing one or more target nucleic acid sequences that are capable of indicating a genetic relationship with a second organism;b) contacting at least one target-protecting molecule that specifically binds to the one or more target nucleic acid sequence with the unlabeled nucleic acid sequences of step a) under hybridizing conditions sufficient to form at least one protected target nucleic acid sequence (PNAS);c) digesting the isolated nucleic acids containing at least one PNAS of step b) with enzymes to form at least one PNAS structure having at least one 5' single-stranded region generated by enzymatic digestion (PNAS/tail);d) hybridizing a reporter probe to the at least one PNAS/tail of step c) wherein the reporter probe binds to a single-stranded region of the at least one PNAS/tail;e) detecting the at least one PNAS/tail as indicative of the presence of the one or more target nucleic acid sequence; andf) determining the genetic relationship between the first and second organism.