Patent ID: 6503721
Filing Date: 2003-01-07
Classification: C12N,C12Q,C40B

Abstract:
A high-throughput method for identifying a ligand that binds a predetermined target RNA sequence, which comprises:(a) selecting as test ligands a plurality of compounds not known to bind to the target RNA sequence; (b) incubating the target RNA sequence in the presence of each of said test ligands to produce a test combination; (c) incubating the target RNA sequence in the absence of a test ligand to produce a control combination; (d) measuring the conformation of the target RNA sequence in each combination; (e) selecting as a ligand any test ligand that causes a measurable change in the target RNA conformation in the test combination relative to the target RNA conformation in the control combination; and (f) repeating steps (b)-(e) with a plurality of said test ligands to identify a ligand that binds to the target RNA sequence.