Patent ID: 8999637
Filing Date: 2015-04-07
Classification: C12N

Abstract:
1. A method for generating a stable modified vaccinia Ankara (MVA) virus vector comprising: providing a vector or vectors containing two nucleotide sequences of at least 800 nucleotides each, encoding proteins with at least 75% identity within a stretch of at least 150 contiguous amino acids; substituting at least 400 nucleotides in one or both of the nucleotide sequence(s) to generate two divergent sequences; wherein the 400 substituted nucleotides do not alter the amino acids encoded by the two divergent sequences; and generating a stable MVA vector comprising the two divergent sequences stably inserted into the MVA genome and that expresses the encoded amino acid sequences, wherein the two divergent sequences share stretches of identity of no more than 9 contiguous nucleotides; wherein the two nucleotide sequences encode a full length RSV-F protein and a truncated RSV-F protein.