Patent ID: 7704715
Filing Date: 2010-04-27
Classification: C12N,C12Y

Abstract:
1. An isolated variant of a parent cytochrome P450 oxygenase, wherein the parent comprises the sequence of SEQ ID NO:13 and wherein the variant is at least 95% identical to SEQ ID NO:13 and has a higher ability than the parent to use peroxide as an oxygen donor and has at least one first mutation in the heme domain in a secondary structural element in the loop between helices B′ and C and at least one second mutation selected from the group consisting of K9, 158, E93, H100, F107, K113, A135, M145, N186, D217, M237, N239, S274, L324, I366, K434, E442, and V446 of SEQ ID NO:13.