Patent ID: 8137925
Filing Date: 2012-03-20
Classification: C07K,C12N,G01N

Abstract:
1. A method for labeling a target protein, the method comprising contacting a fusion protein with a lipoic acid analog, and allowing sufficient time for the lipoic acid analog to be conjugated to the fusion protein via an acceptor polypeptide, in the presence of a lipoic acid ligase polypeptide that comprises an amino acid sequence of a wild-type lipoic acid ligase or a mutant thereof, the mutant being at least 90% identical to SEQ ID NO:11 and having lipoic acid ligase activity; wherein the fusion protein is a fusion of the target protein and the acceptor polypeptide, which comprises an amino acid sequence at least 90% identical to SEQ ID NO: 3, 5, 6, 8, or 10, and wherein the lipoic acid analog comprises an alkyl azide, an alkyne, an alkyl halide, an aryl azide photoaffinity probe, a diazirine photoaffinity probe, a benzophenone photoaffinity probe, or a fluorophore.