Patent ID: 8187828
Filing Date: 2012-05-29
Classification: A61K,A61P,C07K,Y10T

Abstract:
1. A method of screening a PAK1 inhibitor for the treatment of a degenerative joint disease comprising: (a) providing a whole cell comprising a p21-activated kinase (PAK) PAK1 comprising the sequence of SEQ ID NO: 2 or the PAK1 gene comprising the sequence of SEQ ID NO: 1, (b) providing a test compound, (c) contacting said whole cell comprising PAK1 or PAK1 gene with said test compound, and measuring or detecting the influence of said test compound on PAK1 or the PAK1 gene as compared to a control reaction lacking said test compound wherein said measuring or detecting comprises determining the extent that said test compound affects the interaction between said PAK1 or PAK1 gene and Cdc42, Rac1, Wrch-1, Chp or activated p21, or determining the extent that said test compound affects phosphorylation of a substrate thereby identifying an inhibitor for the treatment of a degenerative joint disease.