Patent ID: 6025165
Filing Date: 2000-02-15
Classification: A61K,A61P,C07K

Abstract:
A method of producing a multivalent antigen-binding protein comprising the steps of:(a) producing a composition comprising single-chain molecules, each single-chain molecule comprising:(i) a first polypeptide comprising the binding portion of the variable region of an antibody light chain;(ii) a second polypeptide comprising the binding portion of the variable region of an antibody heavy chain; and(iii) a peptide linker linking said first and second polypentides (i) and (ii); or(iv) a first polypeptide comprising the binding portion of the variable region of an antibody heavy chain;(v) a second polypeptide comprising the binding portion of the variable region of an antibody light chain; and(vi) a peptide linker linking said first and second polypeptides (iv) and (v);(b) dissociating said single-chain molecules;(c) re-associating said single-chain molecules;(d) separating multivalent antigen-binding proteins from said single-chain molecules; and(e) recovering said multivalent proteins.