Patent ID: 7018829
Filing Date: 2006-03-28
Classification: C12N,C12P,Y02E

Abstract:
1. A recombinant yeast cell comprising: i) at least one increased expressible yeast enzyme activity controlling anabolic metabolism of ammonia in said cell as a nutrient source, said increased enzyme activity being selected from the group consisting of: wherein each increased enzyme activity is that of (1) an enzyme, endogenous to said cell, encoded by a nucleic acid coding sequence operably linked to at least one regulatory sequence not natively associated with said nucleic acid coding sequence, whose expression is increased as compared to the expression of the enzyme activity when said nucleic acid coding sequence is associated with its native regulatory sequence or (2) an enzyme, exogenous to said cell, encoded by a nucleic acid coding sequence, operably linked to at least one regulatory sequence, and wherein the recombinant yeast cell is further characterized by ii) a reduced expressible enzyme activity controlling anabolic metabolism of ammonia in said cell as a nutrient source, said reduced enzyme activity being a natively present NADPH-dependent glutamate dehydrogenase activity which reduced activity is reduced compared to the native level of activity.