Patent ID: 7687280
Filing Date: 2010-03-30
Classification: B01J,B01L,C12Q,C40B,G01N,Y10S,Y10T

Abstract:
1. A method of detecting or quantitating a target nucleic acid sequence in a liquid sample, comprising: introducing a liquid sample comprising a target nucleic acid sequence through a channel in a substrate and into a chamber in the substrate which is in fluid communication with the channel, the chamber being pre-loaded with detection reagents before introduction of the liquid sample, the detection reagents comprising a first unlabeled oligonucleotide, a second unlabeled oligonucleotide, and a fluorescently-labeled oligonucleotide; mixing the liquid sample with the detection reagents in the chamber such that the first unlabeled oligonucleotide, the second unlabeled oligonucleotide, and the fluorescently-labeled oligonucleotide are not immobilized in the chamber; reacting the target nucleic acid sequence with both the first unlabeled oligonucleotide and the second unlabeled oligonucleotide, in the chamber, under conditions effective to selectively bind each of the first unlabeled oligonucleotide and the second unlabeled oligonucleotide to the target nucleic acid sequence, and to produce a first reaction product; producing fluorescence in the chamber by a reaction in the chamber involving the first reaction product and the non-immobilized fluorescently-labeled oligonucleotide; and detecting the fluorescence produced in the chamber.