Patent ID: 7494782
Filing Date: 2009-02-24
Classification: C07K,C12Q,G01N,Y10S,Y10T

Abstract:
1. A method of measuring the concentration of factor Vila-antithrombin complexes circulating in a patients plasma, comprising: mixing a quantity of a plasma sample from said patient with a primary capture antibody fixed to a solid phase, wherein said capture antibody specifically binds ability to bind, independent of the presence or absence of calcium ions, to the factor VIIa portion of factor VIIa-antithrombin complexes which is present in said plasma sample, and incubating said mixture under conditions to promote binding between said capture antibody and said factor VIIa-antithrombin complexes in said plasma sample to form capture antibody-factor VIIa-antithrombin complexes and unbound components in the mixture; removing said unbound components; mixing said capture antibody-factor VIIa-antithrombin complexes with a secondary antibody having the ability to bind to the antithrombin portion of said capture antibody-factor VIIa-antithrombin complexes to form capture antibody-factor VIIa-antithrombin-secondary antibody complexes and unbound components in the mixture; removing any unbound components; and determining the amount of capture antibody-factor VIIa-antithrombin-secondary antibody complexes by comparing the amount of capture antibody-factor VIIa-antithrombin-secondary antibody complexes to a standard amount of capture antibody-factor VIIa-antithrombin-secondary antibody complexes determined for factor VIIa-antithrombin complexes of a known concentration.