Patent ID: 6048693
Filing Date: 2000-04-11
Classification: C12N,C12Q

Abstract:
A method of screening for a compound that affects mammalian cell cycle regulatory proteins, comprising:a. administering a compound to a cell line, wherein said cell line comprises genetic information comprisingi. a reporter gene operably linked to a gene expression control sequence, wherein said gene expression control sequence comprises an Upstream Activation Sequence and a promoter, and said Upstream Activation Sequence comprises a DNA region that binds to a transcription control factor that is regulated through phosphorylation by a cyclin/CDK phosphorylation system; andii. an effector gene providing a gene product distinct from said transcription control factor and effective to permit normal cyclin/CDK regulation of said-transcription control factor; andb. analyzing expression of said reporter gene in said cell line, thereby determining whether said compound affects said normal regulation.