Patent ID: 8685946
Filing Date: 2014-04-01
Classification: A61P,C12N,C12Q

Abstract:
1. A method for inhibiting miRNA-mediated RNA silencing of a gene in a cell, comprising, (a) obtaining a cell containing a mature miRNA that directs RNA silencing of the gene, wherein the miRNA is incorporated into RISC in said cell; (b) contacting said cell with a RISC inactivator, wherein said RISC inactivator is a single-stranded, RNA oligonucleotide between about 10-40 nucleotides in length, comprising modified ribonucleotides such that said RNA oligonucleotide is nuclease resistant, and comprising a nucleotide sequence sufficiently complementary to the nucleotide sequence of the mature miRNA to bind said miRNA incorporated into RISC; wherein said RISC inactivator is refractory to RISC-directed endonucleolytic cleavage or translational control, and wherein said RISC inactivator is a stochiometric, irreversibly inhibitor of RISC function, such that miRNA-mediated RNA silencing of the gene is inhibited.