Patent ID: 8617813
Filing Date: 2013-12-31
Classification: A01K,C07K,C12N

Abstract:
1. An in vitro method of identifying a subpopulation of cultured human or mouse embryonic stem (ES) cells expressing Zscan4, comprising: (a) transfecting a population of mouse or human ES cells with an expression vector comprising a Zscan4c promoter operably linked to a nucleotide sequence encoding a reporter, wherein the Zscan4c promoter is selected from group consisting of the nucleic acid sequence as set forth in nucleotides (i) 1-2540 of SEQ ID NO: 28, (ii) 1-2643 of SEQ ID NO: 28, (iii) 1-3250 of SEQ ID NO: 28 and (iv) 1-3347 of SEQ ID NO: 28; and (b) identifying a subpopulation of cells that expresses the reporter gene indicating Zscan-4 is expressed in the subpopulation of stem cells.