Patent ID: 6660527
Filing Date: 2003-12-09
Classification: B01F,B01L,Y10T

Abstract:
A method of using a fluid transfer and mixing collection assembly, comprising:providing a fluid transfer and mixing collection assembly including a base, a test media carried by the base, an inlet for receiving a first fluid, the inlet including an inlet check valve, an outlet including an outlet check valve, a bladder carried by the base between the inlet and the outlet and including an interior with a second fluid therein, and a depressable, flexible member carried by the base between the inlet and the outlet, the flexible member including an interior, a membrane separating the interior of the bladder from the interior of the flexible member, the flexible member including an exterior surface, an interior surface, and a pointed member extending from the interior surface of the flexible member; depressing the flexible member to cause the pointed member to rupture the membrane; releasing the flexible member to impart a negative pressure in the interior of the flexible member to draw the first fluid into the interior of the flexible member through the inlet check valve to mix with the second fluid; depressing the flexible member to impart a positive pressure in the interior of the flexible member to pump the mixed first fluid and second fluid out of the interior of the flexible member through the outlet check valve and be transferred to the test media.