Patent ID: 8633010
Filing Date: 2014-01-21
Classification: C07K,C12N

Abstract:
1. A method for producing a heterologous polypeptide of interest in an Aspergillus host cell comprising: (a) cultivating the Aspergillus host cell comprising a first recombinant nucleic acid sequence and a second recombinant nucleic acid sequence under conditions conducive for the expression of polypeptides encoded by the first recombinant nucleic acid sequence and second recombinant nucleic acid sequence, and wherein the first recombinant nucleic acid sequence encodes a heterologous polypeptide of interest and the second recombinant nucleic acid encodes an inhibitor polypeptide comprising an amino acid sequence having at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 6, and wherein the inhibitor polypeptide is expressed by the Aspergillus host cell with an increased level of the inhibitor polypeptide compared to an Aspergillus host cell not comprising the first recombinant nucleic acid sequence and the second recombinant nucleic acid sequence.