Patent ID: 7022649
Filing Date: 2006-04-04
Classification: C12N

Abstract:
1. A method for preparing a concentrated inoculating composition for use with leguminous plants, comprising me following steps in the order given: a) sterilize and cool a culture medium suitable for supporting the growth of Rhizobium japonicum bacteria; b) add Rhizobium japonicum bacteria to the culture medium and allow to multiply for 46 hours at a temperature of 28 C.; c) cool the result of step b) to a temperature of 10° C. or 11° C.; d) add a solution comprising powdered maltose saccharide, liquid maltose saccharide, lactose saccharide, and potassium sorbate to the combined culture medium and bacteria, said solution being prepared by the following steps: 1) dissolving said powdered maltose saccharide, said liquid maltose saccharide, and said potassium sorbate in 100 liters of water; 2) steriling at 121° C. for 30 minutes; 3) cooling to 20° C.