Patent ID: 7902426
Filing Date: 2011-03-08
Classification: C12N

Abstract:
1. A method for promoting efficiency of transformation of plant embryos, plant calli or cultured plant cells by Agrobacterium , which comprises: a) heating and centrifuging said plant embryos, plant calli or cultured plant cells; and b) contacting said plant cells or plant tissue with the bacterium so that a gene is transferred into the plant embryos, calli, or cells; wherein contact between the plant cells or plant tissue and the bacterium occurs after heating and centrifuging the plant cells or plant tissue, wherein heating is performed at a temperature of 37° C. to 52° C. for 1 minute to 24 hours, wherein said centrifuging is carried out under a centrifugal acceleration of 1000 G to 150,000 G for 1 second to 4 hours and wherein the efficiency of transformation is increased at least twice in comparison to the efficiency of transformation by