Patent ID: 9150902
Filing Date: 2015-10-06
Classification: C08B,C12Q,G01N

Abstract:
1. A method for detecting N-deacetylase/N-sulfotransferase activity in a sample, which comprises detecting N-deacetylase activity in the sample and also detecting N-sulfotransferase activity in the sample, wherein the detection of N-deacetylase activity comprises the steps (a) and (b): wherein the detecting de-N-acetylation is carried out by allowing a protein that binds to de-N-acetylated glucosamine, but not to N-acetylated glucosamine, to contact with said modified polysaccharide, and determining the binding of said protein that binds to de-N-acetylated glucosamine to said modified polysaccharide; wherein the detecting N-sulfotransferase activity comprises steps (c) and (d): wherein the detecting of N-sulfation is carried out by allowing a protein that binds to N-sulfated glucosamine, but not to de-N-sulfated glucosamine, to contact with said modified polysaccharide, and determining the binding of said protein that binds to N-sulfated glucosamine to said modified polysaccharide; wherein the modified polysaccharide comprises a binding substance covalently bound to the non-reducing end or the carboxyl group of N-acetyl heparosan or a derivative thereof, wherein said binding substance interacts directly with said solid phase; and wherein said binding substance is selected from the group consisting of (a) and (b): (a) a protein; and (b) biotin, and wherein the derivative of N-acetyl heparosan is selected from the group consisting of N-desulfated/N-acetylated heparin and N-desulfated/N-acetylated heparan sulfate.