Patent ID: 6211343
Filing Date: 2001-04-03
Classification: A61K,A61P,C07K,Y02A

Abstract:
A method of producing a lactoferrin receptor protein from a bacterial pathogen selected from the group consisting of Neisseria meningitdis, Neisseria gonorrhoeae, Moraxella catarrhalis, Moraxella bovis and Moraxella lacunata, comprising the steps of:(a) providing a solubilized membrane preparation from said bacterial pathogen containing lactoferrin receptor proteins, transferrin receptor proteins and other contaminants, said lactoferrin receptor proteins being a first lactoferrin receptor protein having a molecular weight of 100,000 to 105,000 daltons, as determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), and a second lactoferrin receptor protein having a molecular weight of between 70,000 and 90,000 daltons, as determined by SDS-PAGE,(b) complexing both said first and second lactoferrin receptor proteins with lactoferrin and purifying the resulting complexes free from said transferrin receptor proteins and said other contaminants to provide purified complexes, and(c) separating from the purified complexes both said first and second lactoferrin receptor proteins in the form of a mixture.