Patent ID: 8273348
Filing Date: 2012-09-25
Classification: A61K,A61P,C12N

Abstract:
1. An isolated variant lipase which (a) has at least 90% sequence identity to the sequence of amino acids 1-269 of SEQ ID NO: 2; and (b) has lipase activity; and which, (c) as compared to the sequence of amino acids 1-269 of SEQ ID NO: 2, comprises the four-fold substitution N33Q+T231R+N233R+D254S and is selected from the following: N33Q+T231R+N233R+D254S; N33Q+D96N+T231R+N233R+D254S; N33Q+E210V+T231R+N233R+D254S; N11R+N33Q+E210V+T231R+N233R+D254S; N33Q+G91T+G163K+T231R+N233R+D254S; N11R+N33Q+G91T+G163K+T231R+N233R+D254S; D27N+N33Q+G91T+G163K+T231R+N233R+D254S; N33Q+D111A+T231R+N233R+D254S; D27R+N33Q+G91A+D96E+L97Q+D111A+T231R+N233R+D254S+P256T; N33Q+G91T+N94R+T231R+N233R+D254S; N33Q+W117L+T231R+N233R+D254S; N33Q+G91T+N94R+T231R+N233R+D254S; N33Q+L227F+T231R+N233R+D254S; D27R+N33Q+G91T+D96E+L97Q+D111A+T231R+N233R+D254S+P256T; D27R+N33Q+G91T+D96E+D111A+T231R+N233R+D254S+P256T; D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+S216P+L227G+T231R+N233R+D254S+P256T; D27R+N33Q+G91N+N94R+D111A+S216P+L227G+T231R+N233R+D254S+P256T; D27R+N33Q+G91N+N94R+D111A+S216P+L227G+T231R+N233R+D254S+P256T; D27R+N33Q+G91N+N94R+D111S+A155V+S216P+L227G+T231R+N233R+D254S+P256T; D27R+N33Q+G91N+N94R+D111S+S216P+L227G+T231R+N233R+D254S+P256T; N33Q+D111A+T231R+N233R+D254S; N33Q+D111A+W117L+T231R+N233R+D254S; N33Q+G91T+N94S+D111A+V176I+T231R+N233R+D254S; D27R+N33Q+L227G+T231R+N233R+Q249R+D254S; N33Q+G38A+G91T+G163K+T231R+N233R+D254S; D27V+N33Q+G91A+N94R+D111A+G163K+L227F+T231R+N233R+Q249R+D254S; D27R+N33Q+P136H+L227G+T231R+N233R+Q249R+D254S; N33Q+G91T+D96N+D111A+V176I+T231R+N233R+D254S; N33Q+G91T+N94S+D111A+V176I+T231R+N233R+D254S; D27R+N33Q+G91T+D96E+D111A+G163K+T231R+N233R+D254S+P256T; D27R+N33Q+G91T+D96E+D111A+G163A+T231R+N233R+D254S+P256T; D27R+N33Q+G38A+G91T+D96E+D111A+T231R+N233R+D254S+P256T; N11R+N33Q+G91T+W117I+G163K+T231R+N233R+D254S; D27R+N33Q+G38A+G91T+D96E+D111A+G163K+T231R+N233R+D254S+P256T; N11R+N33Q+G91T+W117I+G163K+T231R+N233R+D254S; D27R+N33Q+G38A+G91T+D96E+D111A+G163A+T231R+N233R+D254S+P256T; D27R+N33Q+V176Q+L227G+T231R+N233R+Q249R+D254S; N33Q+G91A+N94K+D111A+G163K+L227F+T231R+N233R+Q249R+D254S; N11R+N33Q+G91T+G163K+V176Q+T231R+N233R+D254S; D27R+N33Q+W117I+V176Q+L227G+T231R+N233R+Q249R+D254S; N11R+N33Q+G38A+G91T+G163K+T231R+N233R+D254S; D27R+N33Q+G91T+D96E+L97Q+D111A+T231R+N233R+D254S+P256T; N11R+N33Q+G91T+S115L+G163K+T231R+N233R+D254S; N11R+N33Q+G91T+G163K+V176W+T231R+N233R+D254S; E1D+N33Q+G91T+N94R+D111A+W117L+T231R+N233R+D254S; N11R+D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+G163T+S216P+L227G+T231R+N233R+D254S+P256T; D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+G163T+S216P+L227G+T231R+N233R+D254S+P256T; N11R+D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+S216P+L227G+T231R+N233R+D254S+P256T; D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+S216P+L227G+T231R+N233R+D242E+D254S+P256T; D27R+N33Q+G38A+E56Q+D57N+G91N+N94R+D111S+S216P+L227G+T231R+N233R+D254S+P256T; N33Q+G38A+G91T+G163A+T231R+N233R+D254S; N11R+N33Q+190L+G163L+T231R+N233R+D254S; N11R+N33Q+E56Q+G91T+G163K+V176Q+T231R+N233R+D254S; N11R+D27R+N33Q+G91T+D96E+D111A+G163K+T231R+N233R+D254S+P256T; N11R+N33Q+G38A+G91T+G112A+G163A+T231R+N233R+D254S; N11R+N33Q+G91T+G163K+E210D+T231R+N233R+D254S; N11R+N33Q+G91T+G163K+V176T+T231R+N233R+D254S; N11R+N33Q+G91T+G163P+T231R+N233R+D254S; N11R+N33Q+G91M+G163T+T231R+N233R+D254S; N11R+N33Q+G38A+G91T+G163K+V176D+T231R+N233R+D254S; N11R+N33Q+E56Q+G91T+G163K+T231R+N233R+D254S; N11R+N33Q+190L+G163K+T231R+N233R+D254S; D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+S216P+L227G+T231R+N233R+Q249R+D254S+P256T; D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+S216P+E219D+L227G+T231R+N233R+D254S+P256T; N11R+N33Q+190L+G91T+N94S+D96E+G163K+T231R+N233R+D254S; N11R+N33Q+G91T+G163K+V176I+T231R+N233R+D254S; N11R+N33Q+G91T+G163K+V176Q+T231R+N233R+D254S; N11R+N33Q+G91T+G163A+V176T+T231R+N233R+D254S; N11R+N33Q+G91T+G163L+V176I+T231R+N233R+D254S; N11R+N33Q+G91T+G163L+V176T+T231R+N233R+D254S; N11R+N33Q+G91T+G163L+T231R+N233R+D254S; N11R+N33Q+G91T+G163P+T231R+N233R+D254S; N11R+N33Q+G91T+G163P+V176I+T231R+N233R+D254S; N11R+N33Q+G91T+G163L+T231R+N233R+D254S+P256N; D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+G163T+S216P+L227G+T231R+N233R+Q249R+D254S+P256T; D27R+N33Q+L227G+T231R+N233R+Q249R+D254S; N33Q+G38A+G91T+G163K+T231R+N233R+D254S; N8L+N11R+N33Q+G91T+G163K+T231R+N233R+D254S; N11R+N33Q+G38A+G91T+G163P+V176G+T231R+N233R+D254S; N11R+N33Q+G91T+G163K+T231R+N233R+D254S+P256F; N11R+N33Q+G38A+G91T+G156R+G163K+V176T+T231R+N233R+D254S; N11R+N33Q+G91T+G163N+T231R+N233R+D254S; N11R+N33Q+G91T+G163T+T231R+N233R+D254S; N11R+N33Q+G91T+G163W+T231R+N233R+D254S; N11R+N33Q+G91K+G163K+T231R+N233R+D254S; N11R+G23E+N33Q+G91T+G163K+T231R+N233R+D254S; N11R+N33Q+G91T+V141E+G163K+T231R+N233R+D254S; N11R+N33Q+L52R+G91T+G163K+T231R+N233R+D254S; N11R+N33Q+G91T+V141L+G163K+T231R+N233R+D254S; N11R+N33Q+T37K+G91T+G163K+T231R+N233R+D254S; N11R+N33Q+A68V+G91T+G163K+T231R+N233R+D254S; N11R+N33Q+G91T+G163A+V176I+T231R+N233R+D254S; N11R+N33Q+T37M+G91T+G163P+V176T+T231R+N233R+D254S; N11R+N33Q+G91T+G163L+T231R+N233R+D254S; N11R+N33Q+G91T+G163K+T231R+N233R+D254S+P256I; N11R+D27R+N33Q+E56Q+D57N+G91N+N94R+D111S+G163K+S216P+L227G+T231R+N233R+D254S+P256T; N11R+N33Q+G38A+G91T+G163P+V176G+T231R+N233R+D254S; N11R+N33Q+G38A+G91T+G163Q+V176G+T231R+N233R+D254S; N11R+N33Q+G38A+G91T+G163T+V176G+T231R+N233R+D254S; N11R+N33Q+G38A+G91T+N94R+G163P+V176G+T231R+N233R+D254S; E1*+N11R+N33Q+G38A+G91N+N94R+G163P+V176G+T231R+N233R+D254S; E1N+N33Q+G38A+G91T+G163P+V176F+T231R+N233R+D254S; E1N+N33Q+G38A+G91T+G163P+V176F+L227F+T231R+N233R+D254S; E1N+N33Q+G38A+G91T+G163P+V176F+T231R+N233R+D254S+1255A+P256Q; E1N+N11R+N33Q+G38A+G91T+D111A+G163P+V176F+T231R+N233R+D254S; N11R+N33Q+G38A+G91T+D102G+S115L+G163K+T231R+N233R+D254S+P256T; N11R+N33Q+G38A+G91T+S115L+G163K+T231R+N233R+D254S+P256T; E1N+N11R+N33Q+G91T+G163A+T231R+N233R+G246A+D254S; N11R+D27R+N33Q+D57G+G91T+D96E+D111A+G163K+T231R+N233R+D254S+P256T; N11C+N33Q+G91T+G163K+T231R+N233R+D254S; N11L+N33Q+G91T+G163K+T231R+N233R+D254S; N11H+N33Q+G91T+G163K+T231R+N233R+D254S; N11D+N33Q+G91T+G163K+T231R+N233R+D254S; N11R+N33Q+G91T+D96W+G163K+T231R+N233R+D254S; D27R+N33Q+G91T+D96E+L97Q+D111A+G163K+T231R+N233R+D254S+P256T; N11P+N33Q+G91T+G163K+T231R+N233R+D254S; N11R+N33Q+E56Q+G163K+T231R+N233R+D254S; N11R+N33Q+G91T+G163A+T231R+N233R+D254S; N11R+N33Q+G91T+G163P+T231R+N233R+D254S; N11R+N33Q+G91T+G163K+L227G+P229R+T231R+N233R+D254S; N33Q+G91T+K98I+T114I+G163K+T231R+N233R+D254S; N33Q+G91T+K98I+G163K+T231R+N233R+D254S+P256L; and N33Q+G91T+T114I+G163K+T231R+N233R+D254S+P256L.