Patent ID: 8846345
Filing Date: 2014-09-30
Classification: A61K,C07K

Abstract:
1. A method of screening a candidate compound for the ability to inhibit dimer assembly and secretion of a dimeric form of interleukin, comprising the steps of: incubating a cell culture comprising a cell line transfected with an expression vector comprising DNA encoding a subunit of a dimeric form of interleukin under transcriptional control of an ecdysone-inducible promoter with the candidate compound, wherein the subunit is selected from the group consisting of p35 (alpha) subunit of IL-12; p40 (beta) subunit of IL-12; p19 subunit of IL-23; p40 subunit of IL-23; ebi3 subunit of IL-27; and p28 subunit of IL-27; inducing transcription of dimeric interleukin in the cells of the culture by contacting the cells with ecdysone or an ecdysone analog; and assaying the cell culture for the presence of secreted dimeric interleukin.