Patent ID: 6723508
Filing Date: 2004-04-20
Classification: C12Q

Abstract:
A method for determining whether a subject exhibits a GSTT1*0/0 genotype, a GSTT1*A/A genotype, or a GSTT1*A/0 genotype, comprising the steps of:isolating genomic DNA from a subject; performing a PCR on the isolated genomic DNA using a primer pair selected from the group of primer pairs consisting of CAG CCA AGA AGT TCT GAG TCT TG (SEQ ID NO: 15) and ATA TCA GCC AGA GAT CTC TGG G (SEQ ID NO: 16), CCA GCT CAC CCG ATC ATG GCC AG (SEQ ID NO: 19) and CCT TCC TTA CTG GTC CTC ACA TCT C (SEQ ID NO: 20), CAG TTG TGA GCC ACC GTA CCC (SEQ ID NO: 21) and CGA TAG TTG CTG GCC CCC TC (SEQ ID NO: 22), and GTG CCC TTC CCT TAC CCA TC (SEQ ID NO: 23) and GGG TAC CAG TAG TCA GGG ACC TTA (SEQ ID NO: 24); and analyzing results of the PCR for the absence or presence of specific DNA fragments, the absence of specific DNA fragments indicating the subject exhibits the GSTT1*0/0 genotype and the presence of specific DNA fragments indicating the subject exhibits the GSTT1*A/A genotype or the GSTT1*A/0 genotype.