Patent ID: 6316203
Filing Date: 2001-11-13
Classification: A01K,A61K,A61P,C07K,C12Q,G01N

Abstract:
A method for determining whether a ligand is a Y5 receptor agonist which comprises contacting a cell transfected with and expressing DNA encoding the human or rat Y5 receptor with the ligand under conditions permitting activation of the Y5 receptor, and detecting an increase in Y5 receptor activity, so as to thereby determine whether the ligand is a Y5 receptor agonist, wherein such cells prior to being transfected with such DNA do not normally express the human or rat Y5 receptor, and wherein the human Y5 receptor has an amino acid sequence identical to the amino acid sequence shown in FIG. 6 (SEQ ID NO: 4) or that encoded by plasmid pcEXV-hY5 (ATCC Accession No. 75943); and the rat Y5 receptor has an amino acid sequence identical to the amino acid sequence shown in FIG. 4 (SEQ ID NO: 2) or that encoded by plasmid pcEXV-rY5 (ATCC Accession No. 75944).