Patent ID: 7001739
Filing Date: 2006-02-21
Classification: C12N

Abstract:
1. A method of isolating nucleic acid encoding a plant polypeptide active in PTGS comprising the steps of: i) selecting a bait nucleic acid which encodes a bait protein suppressive of PTGS in plants; ii) preparing a cDNA prey library from a plant or plant tissue wherein the plant or plant tissue actively exhibits PTGS; iii) conducting a yeast two-hybrid assay with the bait and prey nucleic acids, wherein prey cDNA that yields a true positive yeast two-hybrid assay result encodes a polypeptide active in PTGS in the plant or plant tissue, wherein the bait nucleic acid comprises a sequence of SEQ. ID. NO. 1 or a sequence of a degenerate code homolog of SEQ. ID. NO. 1.