Patent ID: 7700114
Filing Date: 2010-04-20
Classification: A61K,A61P,C07K,C12N,G01N

Abstract:
1. An expression vector containing a DNA sequence encoding a human papilloma virus (HPV) E6 or E7 protein and a fusion partner, wherein said DNA sequence is characterized by a combination of the following features: (a) at least 20% of the original wild type HPV E6 or E7 codons are exchanged by codons which lead to an enhanced translation in a human cell; (b) the DNA sequence contains a mutation resulting in the production of a truncated non-functional HPV E6 or E7 protein, wherein the mutation is selected from replacement of a codon encoding an amino acid by a stop codon and insertion or deletion of one or more nucleotides resulting in a frame shift mutation and wherein the mutation leads to premature termination of translation; and (c) the fusion partner is HBsAg-S containing at least one mutation to allow for fusion of the HPV E6 or E7 partner to the C terminal end of hepatitis B viral (HBV) envelope protein HBsAg-S.