Patent ID: 6165726
Filing Date: 2000-12-26
Classification: C12Q

Abstract:
A method of sequencing a nucleic acid comprising:a) labeling the nucleic acid with biotin;b) subjecting the nucleic acid of step a) to base specific chemical modifications;c) cleaving the nucleic acid of step b) at the modified bases with hot piperidined) contacting the nucleic acid of step c) with immobilized streptavidin whereby biotin-containing fragments are bound to immobilized streptavidin and unbound fragments are washed off;e) contacting the biotin-containing fragments of d) with sodium hydroxide whereby, the biotin-containing fragments are dissociated into biotin-containing single stranded fragments and their complementary strands;f) extracting the biotin-containing single stranded fragments of step e);g) resolving the fragments of step f) on a denaturing polyacrylamide gel; andh) staining the fragments in the gel of step g) by silver staining whereby the nucleic acid sequence is determined.