Patent ID: 6153376
Filing Date: 2000-11-28
Classification: C12Q,G01N

Abstract:
An antifungal screening assay for identifying compounds that inhibit mannosyltransferases involved in protein O- and N-linked glycosylation, which comprises the steps of:a) providing a mannosyltransferase protein encoded by a Saccharomyces cerevisiae mannosyltransferase encoding gene selected from the group consisting of KRE2/MNT1, YUR1, KTR1, KTR2, KTR3, KTR4, KTR5, KTR6 and KTR7 and further providing a specific substrate of said mannosvltransferase protein;b) subjecting the mannosyltransferase protein and specific substrate of step a) to a compound suspected of having antifungal activity and determining absence or presence on said substrate of specific O- and N-linked oligosaccharides, wherein absence of at least one of said oligosaccharides is indicative of an antifungal compound that specifically inhibits at least one member of the group of step a).