Patent ID: 7563582
Filing Date: 2009-07-21
Classification: A61P,G01N

Abstract:
1. A process for identifying a chemical compound for possible use in a medicament for treating a disorder selected from the group consisting of a cardiovascular disorder, an inflammatory disorder, and a disorder of blood vessels said compound modulating an interaction between i) a human EVH1 (Ena-VASP—( Drosophilia melanogaster enabled vasodilator-stimulated phosphoprotein)—Homology 1) binding domain or a protein having a human EVH1 binding domain and ii) a human EVH1 domain or a protein having a human EVH1 domain, which process comprises: a) bringing a human EVH1 binding domain or a protein having a human EVH1 binding domain which interacts with a human EVH1 domain or a protein having a human EVH1 domain into contact with a chemical compound to be examined on a surface which consists of a solid body and is coated with a human EVH1 binding domain or a protein having a human EVH1 binding domain; b) incubating the mixture according to a) with a monoclonal antibody selected from the group consisting of mAB IE245 and mAB IE273 said antibody specifically binding to a human EVH1 binding domain or a protein having a human EVH1 binding domain or a human EVH1 domain or a protein having a human EVH1 domain or which has an antigen which is fused with or chemically coupled to these domains or proteins; c) incubating the mixture according to b) with a second antibody which is capable of specifically binding the antibody from mixture b), said second antibody having an attached label that can be detected biochemically or physicochemically; d) detecting the label on the second antibody after incubation according to c) by biochemical or physicochemical detection; and e) identifying the chemical compound thereby detected as a potential medicament for treating a disorder selected from the group consisting of a cardiovascular disorder, an inflammatory disorder, and a disorder of blood vessels.