Patent ID: 6903248
Filing Date: 2005-06-07
Classification: C12N

Abstract:
1. A DNA construct comprising in operable linkage: a single promoter sequence; a first DNA molecule which has a length that is insufficient to independently impart resistance to a virus to plants transformed with said first DNA molecule, wherein the first DNA molecule is from a DNA encoding a plant viral coat protein and is at least 110 nucleotides in length; a second DNA molecule, wherein the second DNA molecule is coupled to the first DNA molecule, wherein said second DNA molecule is at least 400 nucleotides in length, wherein the first DNA molecule and the second DNA molecule are heterologous to each other and collectively achieve post-transcriptional silencing of the plant viral coat protein and impart resistance to the virus to plants transformed with said DNA construct; and wherein the single promoter sequence effects transcription of the first DNA molecule and the second DNA molecule; and a single termination sequence which ends transcription of the first DNA molecule and the second DNA molecule.