Patent ID: 6242188
Filing Date: 2001-06-05
Classification: C12N

Abstract:
A composition for releasing nucleic acid from a sample in a form suitable for directly detecting the nucleic acid, sad composition comprising: an aqueous solution comprising one or more lipids selected fom the gout consisting of 3-(2-aminoprpoyl 1,3-dihexadecyloxypropyl) hexadecyl ether, 3-(2aminopropyl,-1-octadecyloxy-3-benzyyloxypropyl) benzyl sulfide and bis(3-bemzyloxypropyl-1-octadecyloxy-3-benzyloxy-2-propyl amine)polyethyleneglycol for releasing nucleic acid from the sample and further comprising one or more of:i) an enzyme(s) to degrade cell structure;ii) a non-ionic membrane fluidizing compound(s); andiii) a metal chelator(s);wherein said aqueous solution is non-denaturing and non-inhibitory of enzymes or proteins used in nucleic acid release, amplification, labeling or detection.