Patent ID: 6787368
Filing Date: 2004-09-07
Classification: G01N

Abstract:
A method for detecting or quantitating an analyte present in a liquid sample, comprisingreacting the liquid sample with an analyte-reaction reagent, by said reacting, generating a solution of a first coil-forming peptide having a selected charge for interacting with a second, oppositely charged coil-forming peptide to form a stable &agr;-helical coiled-coil heterodimer, contacting the first coil-forming peptide generated by said reaction with a biosensor having a detection surface with surface-bound molecules of said second, oppositely charged coil-forming peptide, under conditions effective to form a stable &agr;-helical coiled-coil heterodimer on said detection surface, where binding of the coil-forming peptide to the immobilized coil-forming peptide measurably alters a signal generated by the biosensor, measuring the signal generated by the biosensor to determine whether said coiled-coil heterodimer formation on said detector surface has occurred, and correlating said generated signal to the presence of the analyte in the sample.