Patent ID: 7449289
Filing Date: 2008-11-11
Classification: C12Q

Abstract:
1. A method to detect the presence of a methicillin-resistant Staphylococcus aureus (MRSA) strain in a specimen, comprising: obtaining a sample from said specimen to be analyzed for the presence of a MRSA strain that includes an SCCmec insert containing a mecA gene, said SCCmec being inserted into chromosomal DNA, thereby generating a polymorphic right extremity junction (MREJ) region sequence that includes sequence from both the SCCmec insert right extremity and chromosomal DNA adjoining that right extremity; contacting the sample with at least two primers, wherein the at least two primers amplify the MREJ region sequence of at least one of SEQ ID NOs: 42, 43, 44, 45, 46, 51, 47, 48, 49, 50,171,165,166,167 and/or 168 under conditions of 50 mM KCl, 10 mM Tris-HCl (pH 9.0), 0.1% Triton X-100, 2.5 mM MgCl detecting said amplicon as indicative of the presence of a MRSA strain in said specimen.