Patent ID: 7169894
Filing Date: 2007-01-30
Classification: G01N

Abstract:
1. A method for synthesizing an oligonucleotide encoding a specific amino acid sequence, said method comprising the steps of: (a) contacting an amino acid degradation product from a peptide or protein in a solution with an amino acid binding molecule or particle to which a codon encoding a first amino acid residue is attached, (b) allowing said amino acid degradation product to form a specific complex with said amino acid binding molecule or particle, (c) contacting said complex with a solid-phase material that captures said complex, (d) washing to remove amino acid binding molecules or particles that are not captured by solid-phase material, (e) releasing said attached codon from solid-phase captured complex, and (f) covalently attaching said released codon to a codon encoding a second amino acid residue to form an oligonucleotide encoding at least two linked amino acid residues.