Patent ID: 6353155
Filing Date: 2002-03-05
Classification: C12N

Abstract:
A method for preparing a transgenic plant or seed comprising:a) growing a suspension of Agrobacterium cells until the optical density of the suspension is about 2 to about 2.4 at a wavelength of 600 nanometers, wherein said Agrobacterium cells contain at least one plasmid having a DNA sequence of interest flanked by T-DNA borders; b) diluting said Agrobacterium cells of said suspension with an aqueous medium so that the optical density of the suspension is reduced to about 0.6 to about 1.5, wherein said diluting is not followed by pelleting via centrifugation with subsequent resuspension; and c) treating the flower of said plant with said diluted suspension so that the Agrobacterium cells in said diluted suspension can transform said plant with the DNA-sequence of interest; d) optionally, cultivating said treated plant to produce seed; and e) optionally, growing plants from said seed and selecting for transgenic plants having said DNA sequence of interest.