Patent ID: 8586302
Filing Date: 2013-11-19
Classification: C12Q,Y10T

Abstract:
1. A method for bisulfite conversion of DNA, wherein the bisulfite reaction is conducted at one of (i) within a temperature range from 57° C. to 65° C. and a time range from 4 h and 30 min to 5 h and 30 min; (ii) within a temperature range from 52° C. to 57° C. and a time range from 6 h and 30 min to 7 h and 30 min; (iii) within a temperature range from 57° C. to 65° C. and a time range from 4 h and 45 min to 5 h and 15 min; (iv) within a temperature range from 52° C. to 57° C. and a time range from 6 h and 45 min to 7 h and 45 min; (v) within a temperature range from 59° C. to 62° C. and a time range from 4 h and 45 min to 5 h and 15 min; and (vi) within a temperature range from 54° C. to 56° C. and a time range from 6 h and 45 min to 7 h and 45 min.