Patent ID: 6357163
Filing Date: 2002-03-19
Classification: A61K,A61P,C07H,C07K,C12Q

Abstract:
A method of capturing a nucleic acid comprising contacting under hybridising conditions said nucleic acid with a nucleic acid analogue selected from the group consisting of:(a) a peptide nucleic acid (PNA) comprising a polyamide backbone bearing a plurality of ligands at respective spaced locations along said backbone, said ligands being each independently naturally occurring nucleobases, non-naturally occurring nucleobases or nucleobase-binding groups, each said ligand being bound directly or indirectly to a nitrogen atom in said backbone, and said ligand bearing nitrogen atoms mainly being separated from one another in said backbone by from 4 to 8 intervening atoms; (b) a nucleic acid analogue containing a peptide nucleic acid (PNA) capable of hybridizing to a nucleic acid of complementary sequence to form a hybrid which is more stable against denaturation by heat than a hybrid between the conventional deoxyribonucleotide corresponding to said analogue and said nucleic acid; and (c) a nucleic acid analogue containing a peptide nucleic acid (PNA) capable of hybridizing to a double stranded nucleic acid in which one strand has a sequence complementary to said analogue, so as to displace the other strand from said one strand; immobilised to a solid support, which nucleic acid analogue has a sequence of ligands suitable to hybridise to said nucleic acid.