Patent ID: 6605457
Filing Date: 2003-08-12
Classification: A61K,A61P,C07K,C12N,C12Y

Abstract:
Recombinant neuraminidase free of hemagglutinin, influenza viral RNA or other influenza virus components, obtainable by:a. culturing in a suitable culture medium host cells which are transformed with a neuraminidase expression vector or infected with a virus which is transformed with a neuraminidase expression vector, wherein the expression vector or virus comprises at least part of the coding region of the neuraminidase, having antigenic properties corresponding to naturally occurring neuraminidase, preceded in phase by a type I signal sequence, wherein the expression vector or virus does not include in phase with and immediately preceding the neuraminidase coding region a sequence coding for at least the membrane anchor region of the neuraminidase so that the membrane anchor function is not retained; and b. isolating the expression product neuraminidase from the culture medium.