Patent ID: 8859207
Filing Date: 2014-10-14
Classification: A61K,A61P,C07C,C07D

Abstract:
1. A method of identifying a compound in vitro which inhibits FKBP52-enhanced, androgen receptor (AR)-mediated activity, the method comprising: a) providing an AR tester cell, the tester cell comprising a murine embryonic fibroblast derived from FKBP-52 deficient mice, the tester cell being transfected with a DNA encoding AR, an AR-responsive reporter plasmid, and a vector encoding a FKBP52 protein; b) providing a control cell, the control cell comprising the murine embryonic fibroblast derived from FKBP-52 deficient mice, the control cell being transfected with the DNA encoding AR, the AR-responsive reporter plasmid, and a vector that does not encode FKBP-52; c) contacting the cell of a) and b) with a test compound; d) contacting the cell of a) and b) with an AR agonist; e) incubating the cell of a) and b) for a period of time; f) measuring an amount of AR-responsive reporter expression in a) and b); and g) comparing the amount of AR-responsive reporter expression in the cell of a) and b), wherein the test compound is identified as inhibiting FKBP-52-enhanced, AR-mediated activity when the amount of AR-responsive reporter expression in the cell of a) is less than the amount of AR-responsive reporter expression in the cell of b).