Patent ID: 6602684
Filing Date: 2003-08-05
Classification: A61P,C07K,C12P,Y10S

Abstract:
A method for producing a polypeptide having increased Fc-mediated cellular cytotoxicity in a host cell, comprising:(a) culturing a host cell engineered to express at least one nucleic acid encoding &bgr;(1,4)-N-acetylglucosaminyltransferase III (GnT III) under conditions which permit the production of a polypeptide selected from the group consisting of a whole antibody molecule, an antibody fragment, and a fusion protein that includes the Fc region of an immunoglobulin, wherein said GnT III is expressed in an amount sufficient to modify the oligosaccharides in the Fc region of said polypeptide produced by said host cell and wherein said polypeptide has increased Fc-mediated cellular cytotoxicity as a result of said modification; and (b) isolating said polypeptide having increased Fc-mediated cellular cytotoxicity.