Patent ID: 7883889
Filing Date: 2011-02-08
Classification: A61K,A61P,C12N

Abstract:
1. A method for generation of cancer-testis antigen (CTA) presenting cells comprising: a) collecting peripheral blood mononuclear cells (PBMC) from a subject, b) activating said collected cells with one or more activating agents selected from the group consisting of: pokeweed mitogen (PWM), phytohemagglutinin (PHA)/recombinant human interleukin-2 (rhIL-2), pokeweed mitogen (PMW)/phytohemagglutinin (PHA)/recombinant human interleukin-2 (rhIL-2) and CD40L, c) culturing and optionally expanding ex vivo said activated cells, and d) treating said cultured and optionally expanded cells with a DNA hypomethylating agent four times every 12 hours, replacing half of the culture medium with fresh medium, and then culturing for an additional 48 hours to provide activated PBMC or activated B-lymphocytes concomitantly expressing multiple CTA of the following different families: MAGE-1, MAGE-2, MAGE-3, MAGE-4, NY-ESO-1, GAGE-1-6 and SSX-2, wherein said DNA hypomethylating agent is 5-aza-2′- deoxycytidine.