Patent ID: 7635433
Filing Date: 2009-12-22
Classification: G01N

Abstract:
1. A method for feature alignment in chromatographic systems, comprising: running a first sample through a first separation column; determining a first set of features and a first set of retention times for the first sample run; running a second sample through a second separation column; detecting a second set of features and a second set of retention times for the second sample run; identifying matches between said first set of feature and said second set of features; estimating a systematic shift in features between the first sample run through the first separation column and the second sample run through the second separation column, wherein said estimating comprises performing a polynomial regression calculation on respective retention times from said first and second sets of retention times corresponding to said matches; and adjusting the second set of features detected for the second sample run through the second separation column based on the estimated systematic shift to obtain a third set of features, wherein the third set of features are adjusted features having a third set of retention times, wherein features of said third set that match features of said first set have retention times that differ from said retention times of the matching features in the first set by only residual deviation not caused by systematic deviation.