Patent ID: 9186398
Filing Date: 2015-11-17
Classification: A61K,A61P,C07K,C12N

Abstract:
1. A live attenuated canine parvovirus type 2 (CPV2), wherein said CPV2 comprises a capsid gene coding for both an amino acid other than isoleucine at amino acid position 219 of the capsid protein and an amino acid other than glutamine at amino acid position 386 of the capsid protein; wherein a DNA fragment of a part of the non-capsid region of said CPV2 is replaced by a homologous DNA fragment of a part of the non-capsid region derived from a second parvovirus, wherein said second parvovirus is a canine parvovirus 2, and wherein said homologous DNA fragment of said second parvovirus carries an attenuating mutation in the nonstructural region in the region from position 2061 to 2072, due to the replacement of the DNA sequence of wild-type CPV2 in the region from position 2061 to 2072 encoding the amino acid sequence LTPL (SEQ ID NO: 6) with a DNA sequence encoding the amino acid sequence HVRM (SEQ ID NO: 7).