Patent ID: 7087382
Filing Date: 2006-08-08
Classification: C12N,C12Q

Abstract:
1. An expression library usefull for the detection and isolation of a polypeptide capable of regulating a transduction pathway, the expression library comprising a plurality of expression constructs each including a first coding region encoding one polypeptide of a plurality of polypeptides and a second coding region encoding a reporter molecule, said first and said second coding regions being under the transcriptional control of at least one cis acting regulatory sequence element being regulatable by a trans acting regulator of the transduction pathway, such that when an expression construct of said plurality of expression constructs of said expression library is introduced into a cell, said cell endogenously expressing said trans acting regulator of the transduction pathway, a level of expression of said reporter molecule in said cell within a predetermined range being indicative of regulation of the transduction pathway by a specific polypeptide of said plurality of polypeptides expressed by said cell from said expression construct.