Patent ID: 8951764
Filing Date: 2015-02-10
Classification: C12N,C12P,C12Y

Abstract:
1. A method for producing an isoprenoid precursor or isoprenoid comprising culturing a host cell, which comprises a heterologous nucleic acid encoding an polyprenyl pyrophosphate synthase polypeptide, and saccharifying and fermenting a starch substrate under simultaneous saccharification and fermentation (SSF) conditions in the presence of a glucoamylase, wherein the saccharification and fermentation are performed at pH 6.5 to 8.0, wherein the glucoamylase possesses at least 50% activity at pH 6.0 or above relative to its maximum activity, wherein the glucoamylase is selected from the group consisting of a parent Humicola grisea glucoamylase (HgGA) comprising SEQ ID NO: 3, a parent Trichoderma reesei glucoamylase (TrGA) comprising SEQ ID NO: 6, a parent Rhizopus sp. glucoamylase (RhGA) comprising SEQ ID NO: 9, and a variant thereof, wherein the variant has at least 99% sequence identity to the parent glucoamylase, and wherein the host cell is selected from the group consisting of bacterial cells, fungal cells, and algal cells.