Patent ID: 6118047
Filing Date: 2000-09-12
Classification: C12N

Abstract:
A method of imparting tolerance to an amino acid analog of tryptophan to a maize plant cell comprising:(a) introducing an expression cassette comprising a first DNA segment encoding an exogenous anthranilate synthase operably linked to a promoter other than a native anthranilate synthase promoter functional in a maize plant cell into cells of a susceptible plant to yield transformed plant cells, wherein the anthranilate synthase is substantially resistant to inhibition by free L-tryptophan or an amino acid analog of tryptophan; and(b) expressing the anthranilate synthase encoded by the first DNA segment in the plant cells so as to yield an amount of said anthranilate synthase effective to render the transformed plant cells substantially tolerant to an amount of free L-tryptophan or an amino acid analog of tryptophan that inhibits the growth of the untransformed cells of the susceptible plant.