Patent ID: 9067990
Filing Date: 2015-06-30
Classification: C07K

Abstract:
1. A method for producing an antibody composition comprising a reduced amount of acidic species of the antibody, wherein the antibody comprises the heavy and light chains of adalimumab, the method comprising: (a) contacting a first composition comprising the antibody with a chromatography media, wherein the first composition comprises more than 10% total acidic species of adalimumab, wherein the acidic species of the antibody comprise species selected from the group consisting of charge variants, size variants, fragmentation variants, and any combinations thereof, wherein the acidic species of the antibody do not include process-related impurities selected from the group consisting of host cell proteins, host cell DNA, and media components, wherein the antibody binds to the chromatography media; (b) displacing the antibody bound to the chromatography media with a displacing buffer; and (c) collecting a second composition comprising the displaced antibody, wherein the second composition comprises less than 10% total acidic species of the antibody, thereby producing an antibody composition comprising a reduced amount of acidic species of the antibody.