Patent ID: 9101933
Filing Date: 2015-08-11
Classification: B01L,C12N,C12Q,G01N

Abstract:
1. An integrated gel based microfluidic sample processing device comprising: a substrate having a plurality of micro-channels to form at least a DNA extraction chamber in fluidic cooperation with an amplification chamber which in turn is in fluidic cooperation with a separation and detection channel, the micro-channels containing a DNA extraction material and gel based reaction reagents necessary for processing the sample; the device further comprising electrical contacts for coupling to an external power source, the electrical contacts being capable of inducing electro-kinetic manipulation of the gel based reaction reagents and DNA extracted from the sample throughout the device, wherein the gel based reaction reagents comprise reagents required to conduct a PCR reaction and wherein the PCR reaction reagents are supported in a gel matrix within the microfluidic device so as to localize the PCR reaction reagents while permitting the PCR reaction, wherein the gel matrix is provided by an agarose gel having a concentration of from 1% to 1.5%.