Patent ID: 7976743
Filing Date: 2011-07-12
Classification: A61K,A61P,Y10S

Abstract:
1. A method of making a gas-containing liposome, comprising the following steps in the recited order: a) combining hydrated liposomes with gas under elevated pressure to generate a hydrated gas-liposome dispersion; b) freezing said hydrated gas-liposome dispersion to generate a frozen hydrated gas-liposome dispersion; c) reducing pressure on said frozen hydrated gas-liposome dispersion to atmospheric pressure; and d) thawing said frozen hydrated gas-liposome dispersion to generate a plurality of gas-containing liposomes, wherein a volume of said gas is encapsulated in each of said gas-containing liposomes, and wherein said plurality of gas-containing liposomes contain at least 20 μl of gas per 5 mg of said plurality of gas-containing liposomes.