Patent ID: 8124191
Filing Date: 2012-02-28
Classification: B82Y,G01N,H01B,H01M,Y02E

Abstract:
1. A method for single-side formation of a planar lipid bilayer in a single-side membrane forming apparatus comprising a first fluid chamber having a bottom wall and a sidewall, and a second fluid chamber having a sidewall and a barrier wall separating the first fluid chamber from the second fluid chamber and including at least one nanopore formed therein, the method comprising: adding an electrolyte to the first fluid chamber to establish a level of electrolyte in the first fluid chamber; adding an electrolyte to the second fluid chamber; adding a lipid solution to the electrolyte in the first fluid chamber to form a lipid layer on a surface of the electrolyte; and adjusting the level of electrolyte within the first fluid chamber relative to the nanopore such that the lipid layer is carried across a surface of the barrier wall, causing spontaneous single-side formation from the first fluid chamber of a planar lipid bilayer across the nanopore.