Patent ID: 9045732
Filing Date: 2015-06-02
Classification: A61K,A61P,C12N

Abstract:
1. A method of producing a human RPE preparation suitable for transplantation into the eye of a human patient in need thereof comprising: (a) differentiating in vitro human pluripotent cells that express Oct-4, alkaline phosphatase, SSEA-3, SSEA-4, TRA-I-60, and TRA-I-81 under conditions that do not maintain the undifferentiated state of the pluripotent cells to obtain a heterogeneous population of human cells including pigmented cells comprising brown pigment dispersed in their cytoplasm; (b) isolating and culturing said pigmented cells from the heterogeneous cell population to obtain a monolayer comprising cells having a cobblestone, polygonal, epithelial-like appearance and brown pigment dispersed in their cytoplasm, and expressing RPE65 and bestrophin, and (c) isolating and purifying the RPE cells to form a composition suitable for human therapy.