Patent ID: 7993851
Filing Date: 2011-08-09
Classification: G01N

Abstract:
1. An immunoassay method for determining the presence or concentration of an analyte, the method comprising: (a) contacting a test sample suspected of having said analyte with a lysis reagent to form a homogeneous lysis mixture that can be assayed without a subsequent separation step, the lysis reagent comprising a glycol selected from the group consisting of ethylene glycol, propylene glycol, and an analog thereof, wherein immunoglobulins of an animal species that is the same as the animal species of a capture antibody that binds the analyte are included in the lysis reagent or added to the lysis mixture for neutralizing potential interference; (b) contacting the lysis mixture with a solid phase comprising a solid-phase affixed binding partner for the capture antibody and with the capture antibody, wherein the capture antibody is not pre-bound to the binding partner, and said contacting is carried out under conditions suitable for the capture antibody to bind to the analyte and to the solid phase-affixed binding partner, whereby the capture antibody and analyte form a solid phase-affixed immune complex; (c) contacting the solid phase with a detection agent under conditions suitable for the detection agent to bind to the solid phase-affixed immune complex; and (d) detecting binding of the detection agent to the solid phase affixed immune complex.