Patent ID: 7541151
Filing Date: 2009-06-02
Classification: G01N

Abstract:
1. A method of detecting the presence or absence of a G protein-coupled receptor (GPCR) ligand in a test sample, comprising the steps of: (a) providing a cell comprising a GPCR and a detectably labeled arrestin, wherein the cellular distribution of the detectably labeled arrestin changes in response to activation of the GPCR; (b) determining the cellular distribution of the detectably labeled arrestin in the absence of a test sample; (c) exposing the cell to the test sample; (d) determining the cellular distribution of the detectably labeled arrestin in the presence of the test sample; and (e) comparing the cellular distribution of the detectably labeled arrestin in the presence of the test sample to the cellular distribution of the detectably labeled arrestin in the absence of the test sample, wherein a change in the cellular distribution of the detectably labeled arrestin in the presence of the test sample in response to a GPCR ligand as compared to the cellular distribution of the detectably labeled arrestin in the absence of the test sample indicates the presence of a GPCR ligand in the test sample.