Patent ID: 6174998
Filing Date: 2001-01-16
Classification: C07H

Abstract:
A compound of formula V: ##STR3##whereinR.sub.1, R.sub.2 and R.sub.3 are independently selected from the group consisting of hydrogen, halogen, hydroxy, thio, --(CH.sub.2).sub.n --SH wherein n=1-10, --S--(CH.sub.2).sub.n CH.sub.3 wherein n=0-10, amino, --(CH.sub.2).sub.n --NH.sub.2 wherein n=1-15, --(CH.sub.2).sub.n C(=O)--NH.sub.2 wherein n=1-15, --NH--(CH.sub.2).sub.n CH.sub.3 wherein n=0-15, --NH--(CH.sub.2).sub.n NH.sub.2 wherein n=1-15, --CH=(CH.sub.2).sub.n --NH.sub.2 wherein n=1-6, --C.tbd.C--(CH.sub.2).sub.n --NH.sub.2 wherein n=1-10, carboxy, C.sub.1 -C.sub.6 alkyl, C.sub.2 -C.sub.6 alkenyl, C.sub.2 -C.sub.6 alkynyl, C.sub.6 H.sub.5 --, --O--(CH.sub.2).sub.n CH.sub.3 wherein n=0-6, --O--C.sub.6 H.sub.5, --(CH.sub.2).sub.n C.sub.6 H.sub.5 wherein n=1-6, --O--(CH.sub.2).sub.n C.sub.6 H.sub.5 wherein n=1-6, and a reporter group,R.sub.5 and R.sub.6 are independently selected from the group consisting of hydrogen, hydroxy, thio, --(CH.sub.2).sub.n --SH wherein n=1-10, --S--(CH.sub.2).sub.n CH.sub.3 wherein n=0-10, amino, --(CH.sub.2).sub.n --NH.sub.2 wherein n=1-15, --(CH.sub.2).sub.n C(=O)--NH.sub.2 wherein n=1-15, --NH--(CH.sub.2).sub.n CH.sub.3 wherein n=0-15, --NH--(CH.sub.2).sub.n NH.sub.2 wherein n=1-15, --CH=(CH.sub.2).sub.n --NH.sub.2 wherein n=1-6, --C.tbd.C--(CH.sub.2).sub.n --NH.sub.2 wherein n=1-10, --O--(CH.sub.2).sub.n CH.sub.3 wherein n=0-6, --O--C.sub.2 -C.sub.6 alkenyl, --O--C.sub.2 -C.sub.6 alkynyl, a protecting group and a reporter group,R.sub.7 is selected from the group consisting of hydrogen, hydroxy, thio, --(CH.sub.2).sub.n --SH wherein n=1-10, --S--(CH.sub.2).sub.n CH.sub.3 wherein n=0-10, amino, --(CH.sub.2).sub.n --NH.sub.2 wherein n=1-15, --(CH.sub.2).sub.n C(=O)--NH.sub.2 wherein n=1-15, --NH--(CH.sub.2).sub.n CH.sub.3 wherein n=0-15, --NH--(CH.sub.2).sub.n NH.sub.2 wherein n=1-15, --CH=(CH.sub.2).sub.n --NH.sub.2 wherein n=1-6, --C.tbd.C--(CH.sub.2).sub.n --NH.sub.2 wherein n=1-10, a phosphoramidite or H-phosphonate group, an ester or amide residue that can be cleaved and a reporter group,or R.sub.6 and R.sub.7 together form a further bond between C-2' and C-3' or an acetal group,R.sub.8 is selected from the group consisting of hydrogen or a hydroxy, thio, --(CH.sub.2).sub.n --SH wherein n=1-10, --S--(CH.sub.2).sub.n CH.sub.3 wherein n=0-10, an amino group, --(CH.sub.2).sub.n --NH.sub.2 wherein n=1-15, --(CH.sub.2).sub.n C(=O)--NH.sub.2 wherein n=1-15, --NH--(CH.sub.2).sub.n CH.sub.3 wherein n=0-15, --NH--(CH.sub.2).sub.n NH.sub.2 wherein n=1-15, --CH=(CH.sub.2).sub.n --NH.sub.2 wherein n=1-6, and --C.tbd.C--(CH.sub.2).sub.n --NH.sub.2 wherein n=1-10, andR.sub.9 is selected from the group consisting of a triphosphate group, an alpha, beta or gamma thiophosphate analogue of a phosphoric acid ester, and a protecting group,or a tautomer or salt thereof,wherein at least one of R.sub.1, R.sub.2, R.sub.3, R.sub.5, R.sub.6 and R.sub.7 is a reporter group which is bound to the pyrimidine or furanose ring via a linker group, wherein the linker group contains an alkynyl group.