Patent ID: 7582432
Filing Date: 2009-09-01
Classification: C07B,C07C,C07D,C07F,C07H,C09B,C12Q,C40B,G01N

Abstract:
1. A method for detecting a nucleic acid target sequence, said method comprising: (a) contacting said target sequence with a detector nucleic acid; comprising a single-stranded target binding sequence, said detector nucleic acid having linked thereto,  wherein said detector nucleic acid is in a conformation allowing donor-acceptor energy transfer between said fluorophore and said quencher when said fluorophore is excited; (b) hybridizing said target binding sequence to said target sequence, thereby altering said conformation of said detector nucleic acid, causing a change in a fluorescence parameter; and (c) detecting said change in said fluorescence parameter, thereby detecting said nucleic acid target sequence.