Patent ID: 6051760
Filing Date: 2000-04-18
Classification: C07K,C12N,Y02A

Abstract:
A nucleic acid molecule comprising a maize-optimized nucleic acid coding sequence that encodes a CryIB protein, wherein said maize-optimized nucleic acid coding sequence is produced by a method comprising:(a) determining the amino acid sequence of said CryIB protein;(b) reverse translating said amino acid sequence into said maize-optimized nucleic acid coding sequence, wherein said maize-optimized nucleic acid coding sequence comprises a sufficient number of the single codons that most frequently encode each amino acid in maize, wherein said maize-optimized nucleic acid coding sequence has at least about 60% G+C content, and wherein the single codons that most frequently encode each amino acid in maize are determinable by (i) pooling a plurality of gene sequences from maize, (ii) calculating a codon usage profile from said pooled maize gene sequences, and (iii) determining which single codon most frequently encodes each amino acid in maize; and(c) synthesizing said maize-optimized nucleic acid coding sequence.