Patent ID: 9163279
Filing Date: 2015-10-20
Classification: B01L,C12Q

Abstract:
1. A self-contained device for carrying out an assay to detect a target nucleic acid in a sample, said device comprising: a central block; a first well in the central block, the first well configured to conduct a nucleic acid amplification reaction of a target nucleic acid; a lateral flow assay device in the central block; a first channel in the central block extending from said first well to the lateral flow assay device; a diluent well in the central block, the diluent well being connected to the first well by a second channel in the central block, and wherein the first well is of smaller volume than a volume of the diluent well; a sealed container of diluent received in the diluent well; a piercing means, wherein the piercing means is arranged such that the sealed container of diluent is punctured and opened to dispense the diluent into the diluent well when pressure is applied to the sealed container of diluent or the piercing means after completion of the nucleic acid amplification reaction; a cover plate engaged to the central block to close the first well, the first channel, the second channel, the lateral flow assay device, and the diluent well; and a sample well configured to receive a sample, the sample well connected to the first well, and wherein a sample well cover is configured to seal the sample well closed to close the device to additional fluids; wherein the lateral flow assay device is arranged to receive the sample from said first channel on a bibulous membrane thereof, and wherein said membrane contains elements that are configured to detect said target nucleic acid; and wherein, by applying pressure to the sealed container of diluent or the piercing means after completion of the nucleic acid amplification reaction, the diluent from the diluent well floods the first well by the second channel to force the sample from the first well to the lateral flow assay device.