Patent ID: 7601803
Filing Date: 2009-10-13
Classification: C07K,Y02A

Abstract:
1. A mutagenized bovine gamma-II-crystallin polypeptide with a new binding activity towards a binding partner, wherein amino acids on a surface of a bovine gamma-II-crystallin of SEQ ID NO: 22 is mutagenized, and further wherein: the amino acids that are mutagenized are selected from the group consisting of Lys 3, Thr 5, Tyr 7, Cys 16, Glu 18, Ser 20, Arg 37, and Asp 39 of the bovine gamma-II-crystallin of SEQ ID NO: 22; said beta-sheet, said beta-strands, and said amino acids are located on a surface of said gamma-crystallin polypeptide and are thus accessible to a solvent or a binding partner; and the mutagenizing is selected from the group consisting of an insertion, a deletion, a substitution, and combinations thereof, such that the mutagenized gamma-crystallin polypeptide has a new binding activity towards a binding partner, with the proviso that the gamma-crystallin polypeptide without substitution, deletion, insertion, or combinations thereof has no binding activity at the surface of the beta-sheet structure wherein the amino acids are mutagenized, and after substitution, deletion, insertion, or combinations thereof at the surface of the beta-sheet structure, the gamma-crystallin polypeptide has a new binding activity towards a binding partner.