Patent ID: 6534316
Filing Date: 2003-03-18
Classification: G01N,Y10T

Abstract:
A method for selectively detecting fructose in a sample, wherein one or more sugars other than fructose are present in the sample, said method comprising the steps of:(a) preparing a solution, in a mixed water/aprotic polar solvent, of at least a portion of the sample and a compound selected from the group consisting of compounds having structure 7 or having structure 8 as follows: wherein R=H, C1-C4 alkyl, halogen, thio, hydroxy, C1-C4 ether, C1-C4 carbonyl, C0-C4 amino, C0-C4 amido, C1-C4 ester, aryl, boronic acid, or metal, and wherein the different Rs may be the same or different; and wherein said compound has previously been held at a temperature and for a time sufficient to induce a color in said compound; and (b) measuring the absorbance or fluorescence of the solution at a visible or ultraviolet wavelength or wavelengths at which the absorbance or fluorescence is a function of the fructose concentration.