Patent ID: 8741628
Filing Date: 2014-06-03
Classification: C12N,C12P,Y02E,Y02P

Abstract:
1. An integrated process for the production of oil bearing Chlorella variabilis for lipid extraction utilizing by-products of Jatropha methyl ester (JME) production from whole seeds of Jatropha and the process comprising the steps of: i) providing deoiled cake as by-product of ii) hydrolyzing the deoiled cake as provided in step (i) with hot acidic aqueous solution followed by adjusting pH in the range of 5.5 to 8.5 with alkaline materials to obtain a nitrogen-rich iii) providing crude glycerol containing methanol-depleted glycerol layer containing by product of JME production obtained by washing crude fatty acid methyl ester obtained by transesterification of oil obtained from iv) adding 1-5% (w/v) of crude glycerol containing methanol-depleted glycerol as provided in step (iii) to 1-10% (v/v) of v) inoculating 1-10% (v/v) of vi) optionally inoculating 1-10% (v/v) of vii) drying the biomass as obtained in step (v to vi) in sun or directly using the wet biomass for lipid extraction; viii) extracting the lipid from biomass as obtained in step (vii).