Patent ID: 7326826
Filing Date: 2008-02-05
Classification: A01H,C12N

Abstract:
1. A method of selecting for a grape somatic embryo having resistance to a plant fungal toxin, said method comprising the steps of: (a) culturing a perennial grape embryogenic cell culture in a first liquid culture medium to produce a cellular suspension culture, said first liquid culture medium comprising a plant growth regulator and said plant fungal toxin, wherein said toxin is included in a plant pathogen culture filtrate prepared from a fungus; (b) recovering a grape cell or grape cell cluster from said cellular suspension culture; (c) culturing said grape cell or grape cell cluster of step (b) in a second liquid culture medium; (d) selecting a grape cell or grape cell cluster having resistance to said plant fungal toxin; and (e) culturing the selected grape cell or grape cell cluster of part (d) in a third culture medium to produce a grape somatic embryo having resistance to said fungal toxin; wherein the toxin is from a fungus selected from the group consisting of