Patent ID: 6028187
Filing Date: 2000-02-22
Classification: C12Q

Abstract:
A probe mix comprising:a) a nucleic acid hybridization assay probe which detects the presence of Listeria monocytogenes comprising an oligonucleotide 15 to 100 bases in length having at least 14 out of 17 contiguous bases perfectly complementary to a Listeria monocytogenes nucleic acid variable region, said variable region consisting of a nucleic acid sequence selected from the group consisting of:5' CTGAGAATAGTTTTATGGGATTAGCTCC (SEQ ID NO: 1), and5' GGAGCTAATCCCATAAAACTATTCTCAG (SEQ ID NO: 4);wherein under stringent conditions said probe hybridizes to Listeria monocytogenes 16S rRNA or rDNA to form a detectable probe:target duplex, but said probe does not hybridize to non-target nucleic acid from Listeria grayi, Listeria innocua, Listeria ivanovii, Listeria murrayi, Listeria seeligeri, Listeria welshimeri and Brochothrix thermosphacta to form a detectable probe:non-target duplex; andb) a helper probe comprising a nucleotide base sequence selected from the group consisting of: 5' GGCGAGTTGCAGCCTACAATCCGAA (SEQ ID NO: 2), and the RNA version thereof.