Patent ID: 7700287
Filing Date: 2010-04-20
Classification: C12Q

Abstract:
1. A method of sequencing a nucleic acid, comprising the steps of: a) forming a first, second, third, and fourth reaction mixture such that: b) combining each of the first, second, third, and fourth reaction mixtures with a template polynucleotide, a polymerase, a mixture of nucleoside triphosphates suitable for enzymatic primer extension, and at least one blocking probe, wherein said template polynucleotide comprises, in a 3′ to 5′ direction, a priming sequence complementary to the sequencing primer, a sequence of interest, and a blocking sequence complementary to the blocking probe; c) enzymatically extending the sequencing primer in the first, second, third and fourth reaction mixtures to form a plurality of differently-sized primer extension products; d) separating the primer extension products based upon their sizes; and e) determining therefrom the sequence of the sequence of interest.