Patent ID: 8304216
Filing Date: 2012-11-06
Classification: C12N,C12P

Abstract:
1. A method of producing an optically active alcohol, said method comprising the step of allowing a polypeptide or a transformant to act on a carbonyl group-containing compound, wherein the polypeptide is defined below under (a), (b), or (c): (a) a polypeptide comprising the amino acid sequence shown in the sequence listing under SEQ ID NO: 2; (b) a polypeptide comprising an amino acid sequence derived from the amino acid sequence shown in the sequence listing under SEQ ID NO: 2 by substitution, insertion, deletion and/or addition of one or no more than 20 amino acids, said polypeptide having an activity of asymmetrically reducing N-Boc-2-amino-3-cyclohexyl-3-oxopropionic acid ester represented by the formula (1): to form the corresponding (2R, 3R) —N-Boc-2-amino-3-cyclohexyl-3-hydroxypropionic acid ester represented by the formula (4): (wherein R is a substituted or unsubstituted alkyl group or aryl group); (c) a polypeptide comprising an amino acid sequence showing 85% or higher sequence homology to the amino acid sequence shown in the sequence listing under SEQ ID NO: 2, said polypeptide having an activity of asymmetrically reducing the N-Boc-2-amino-3-cyclohexyl-3-oxopropionic acid ester represented by the formula (1) to form the corresponding (2R, 3R) —N-Boc-2-amino-3-cyclohexyl-3-hydroxypropionic acid ester represented by the formula (4), and  wherein the transformant is obtained by transforming host cells with a vector comprising a DNA coding for the polypeptide, or a DNA defined below under (a), (b), or (c):  wherein the stringent condition is the condition where hybridization is carried out in an aqueous solution comprising 75 mM trisodium citrate, 750 mM sodium chloride, 0.5% sodium dodecyl sulfate, 0.1% bovine serum albumin, 0.1% polyvinylpyrrolidone and 0.1% Ficoll 400 at 65° C., followed by washing with an aqueous solution containing 15 mM trisodium citrate, 150 mM sodium chloride and 0.1% sodium dodecyl sulfate at 65° C.;