Patent ID: 6030827
Filing Date: 2000-02-29
Classification: C12Q,Y10S

Abstract:
A microfabricated device for detecting an analyte molecule in a co-reactant-containing sample comprising(a) a transducing element;(b) a first layer contacting the surface of said transducing element, said first layer comprising a support matrix containing at least one catalyst capable of catalyzing the conversion of said analyte and co-reactant into a reaction product detectable by said transducing element;(c) a second layer in contact with said first layer, second layer permitting transport of said analyte molecule and co-reactant; and(d) a third layer covering said first and second layers, said third layer being permeable to co-reactant but substantially impermeable to said analyte molecule and containing at least one microfabricated aperture extending there through, which permits transport of said analyte to said first layer.