Patent ID: 6132979
Filing Date: 2000-10-17
Classification: G01N

Abstract:
A cytotoxicity testing method comprising the steps of:(a) sowing adherent cells at a cell density on a glass base having a cell adhering film pattern comprised of a plurality of cell adhering films, wherein one to two of said cells selectively adheres to each of said films;(b) thereafter culturing said cells in a culture fluid containing a chemical substance to be tested;(c) staining said cultured cells with a liquid dye, said dye comprising a dye for staining live cells, a dye for staining injured cells, a dye for staining dead cells, or a dye for staining live and a dye for staining dead cells to obtain stained, chemically-treated cells; and(d) counting live cells and dead cells one by one by observing said base to thereby determine a survival rate of said cells, whereby toxicity of said chemical substance is quantitated on the basis of a microscopic visualization of each of said stained, chemically-treated cells adherent to each of said films.