Patent ID: 6552185
Filing Date: 2003-04-22
Classification: C12P

Abstract:
A method for the recovery from a complex mixture of a compound of formula (I): R0 is hydrogen or C1-3 alkoxy; Y is CH2, oxygen, sulphur, or an oxidised form of sulphur; R1 is selected from the group consisting of hydrogen, hydroxy, halogen, C1-3 alkoxy, R2 is selected from the group consisting of adipyl, succinct, 2-(carboxyethylthio)acetyl, 3-(carboxyethylthio)propionyl, higher alkyl saturated dicarboxylic acids and higher alkyl unsaturated dicarboxylic acids, wherein said mixture comprises in addition to the compound of formula (I), 6-aminopenicillanic acid (6-APA) and optionally one or more additional N-substituted &bgr;-lactam compounds, comprising the steps of: (a) acidifying the complex mixture to a pH below 6.5 and maintaining the mixture below said pH at a temperature of between 50Â° C. and 130Â° C.; and/or (b) contacting the complex mixture with a carbon dioxide source; and (c) subjecting the mixture obtained after steps (a) and/or (b) to chromatography to obtain the compound of formula (I).