Patent ID: 6488855
Filing Date: 2002-12-03
Classification: B01J

Abstract:
A method for separating a mixture of polynucleotides, comprisinga) applying a mixture of polynucleotides to separation medium comprising particles selected from the group consisting of silica, silica carbide, silica nitrite, titanium oxide, aluminum oxide, zirconium oxide, carbon, insoluble polysaccharide, and diatomaceous earth, the particles having separation surfaces which are coated with a hydrocarbon or non-polar hydrocarbon substituted polymer, or have substantially all polar groups reacted with a non-polar hydrocarbon or substituted hydrocarbon group, wherein said surfaces are non-polar and the particles are substantially free from multivalent cations which are free to interfere with polynucleotide separation, wherein said polynucleotides comprise single-stranded DNA; b) eluting said mixture of polynucleotides.