Patent ID: 7977077
Filing Date: 2011-07-12
Classification: C12P

Abstract:
1. A recombinant cell for aminoshikimate biosynthesis comprising: (a) a recombinant 3-keto-D-glucose-6-phosphate (3KG6P) dehydrogenase comprising the amino acid sequence of SEQ ID NO:2, (b) a recombinant 3-keto-D-glucose-6-phosphate (3KG6P) transaminase comprising the amino acid sequence of SEQ ID NO:4, and (c) a recombinant 4-amino-3,4-dideoxy-D-arabino-heptulosonic acid 7-phosphate (aminoDAHP) synthase comprising the amino acid sequence of SEQ ID NO:8, wherein the recombinant cell is capable of catalyzing conversion of glucose-6-phosphate (G6P) to 3-keto-D-glucose-6-phosphate (3KG6P), 3KG6P to kanosamine-6-phosphate (K6P), K6P to 1-imino-1-deoxy-D-erythrose-4-phosphate (iminoE4P), iminoE4P to 4-amino-3,4-dideoxy-D-arabino-heptulosonic acid 7-phosphate (aminoDAHP), and aminoDAHP to aminoshikimate.