Patent ID: 8011133
Filing Date: 2011-09-06
Classification: A01G,G01N,G06K,G06Q

Abstract:
1. A method for high throughput quantification of pre-shed pollen of a plant comprising: a. providing a plant having a portion including pre-shed pollen; b. placing the portion of the plant in a liquid; c. blending, in a blender having a rotatable blender blade, the liquid and portion of the plant to create a relatively homogenous liquid suspension; d. extracting a sample of the liquid suspension through a filter while the blender is running, the filter having the characteristic of passing the pollen; e. placing the sample in a container; f. distributing the pollen substantially uniformly in the sample; g. acquiring a magnified digital image of a known portion of the sample, h. counting objects in the magnified digital image which appear to be pollen.