Patent ID: 6228620
Filing Date: 2001-05-08
Classification: A61K,C07K,C12N,Y10S

Abstract:
A nucleic acid composition for introducing nucleic acid into a eukaryotic host cell to obtain expression of a recombinant protein lacking all or a portion of the B domain of human Factor VIII, wherein said recombinant protein consists of:a first amino acid sequence having at least 90% sequence identity with the contiguous amino acid sequence of amino acids 1 to 740 or amino acids 1 to 741 of the native, mature A domain of human Factor VIII as encoded by the polynucleotide present in plasmid pSVF8-200 (ATCC No. 40190); anda second amino acid sequence having at least 90% sequence identity with the contiguous amino acid sequence of amino acids 1649, plus or minus 10 amino acids, through amino acid 2332 of the native, mature C domain of human Factor VIII as encoded by the polynucleotide present in plasmid pSVF8-200 (ATCC No. 40190);wherein said nucleic acid encodes said first and second amino acid sequences and does not encode the complete B domain of human Factor VIII,and further wherein the recombinant protein is capable of coagulation activity in a coagulation activity assay.