Patent ID: 7781175
Filing Date: 2010-08-24
Classification: A61P,G01N,Y10S

Abstract:
1. A method of screening (I) a compound or its salt that alters the binding property of a G protein-coupled receptor protein or a salt thereof, to a bile acid, or (II) a compound or its salt that modulates signal transduction induced by binding of said receptor protein or a salt thereof to a bile acid, wherein said method comprises: a) contacting a bile acid with the G protein-coupled receptor protein containing the amino acid sequence of SEQ ID NO: 1 or a protein containing an amino acid sequence having at least 80% homology to the amino acid sequence of SEQ ID NO: 1 and having the same ligand binding activity as the receptor consisting of the amino acid sequence of SEQ ID NO: 1 or the salts thereof, and measuring (i) the amount of said bile acid bound to said G protein-coupled receptor protein or said protein or salts thereof, or (ii) signal transduction mediated by said G protein-coupled receptor protein or said protein or salts thereof; and b) contacting a bile acid and a test compound with the G protein-coupled receptor protein containing the amino acid sequence of SEQ ID NO: 1 or a protein containing an amino acid sequence having at least 80% homology to the amino acid sequence of SEQ ID NO: 1 and having the same ligand binding activity as the receptor consisting of the amino acid sequence of SEQ ID NO: 1 or the salts thereof, and measuring (i) the amount of said bile acid bound to said G protein-coupled receptor protein or said protein or salts thereof, or (ii) signal transduction mediated by said G protein-coupled receptor protein or said protein or salts thereof; and c) comparing binding property or signal transduction between a) and b).