Patent ID: 6080576
Filing Date: 2000-06-27
Classification: C12N,C40B

Abstract:
A genetically engineered vector comprising:a) a 5' gene trap cassette comprising in operable combination:1) a splice acceptor;2) a first exon sequence located 3' to said splice acceptor, said first exon encoding a marker enabling the identification of a cell expressing said exon; and3) a polyadenylation sequence defining the 3' end of said first exon;b) a 3' gene trap cassette located 3' to said polyadenylation sequence comprising in operable combination:1) a first promoter;2) a second exon sequence located 3' from and expressed by said promoter, said second exon being derived from a naturally occuring eukaryotic gene, said second exon not encoding an activity conferring antibiotic resistance and said second exon not being a reporter gene;3) a splice donor sequence defining the 3' region of the exon said splice donor sequence being derived from a naturally occuring eukaryotic gene; andwherein said vector docs not encode a promoter mediating the expression of said first exon, and wherein said vector does not encode a sequence that mediates the polyadenylation of an mRNA transcript encoded by said second exon sequence and expressed by said first promoter.