Patent ID: 6887714
Filing Date: 2005-05-03
Classification: B01F,B01J,B01L,C12Q,F04B,G01N

Abstract:
1. A method for quantitating or detecting the presence or absence of an analyte comprising: addition of a sample to a microassay structure having at least one electrode and an analyte binding material; allowing an analyte in said sample to bind to said analyte binding material in said microassay structure; rinsing said microassay structure such that said analyte remains bound to said analyte binding material while the remainder of said sample is removed; addition of an analyte binding molecule having a carrier species attached wherein said carrier species includes an electroactive species capable of generating a current by either accepting or transmitting one or more electrons to the at least one electrode and wherein said carrier species may be activated such that it releases the electroactive species; allowing said analyte binding molecule to bind to said analyte; activating said carrier species wherein said activation of said carrier species is selected from the group consisting of change in temperature, change in pH, and addition of an activating compound; and, measuring a current through said microassay structure by means of the at least one electrode within the microassay structure thereby quantitating or detecting the presence or absence of the analyte.