Patent ID: 7384387
Filing Date: 2008-06-10
Classification: C12Q,G01N

Abstract:
1. A method of performing high throughput mass spectrometry screening, the method comprising: (i) providing cells that have been transfected or transformed with one or more members of a library of related genes; (ii) growing the cells in vitro in a biological matrix to express said members of the library of related genes; (iii) separating the cells or cell debris thereof from one or more component of interest using centrifugation or filtration in a parallel fashion to provide samples comprising the component(s) of interest; (iv) performing flow-injection analysis using electrospray tandem mass spectrometry on the samples from step (iii) to obtain mass-to-charge ratio data for the component of interest, wherein the component(s) of interest is selected from the group consisting of an inorganic ion, a secondary metabolite, a protein binding molecule, a carbohydrate, a carbohydrate binding molecule, an enzyme, an enzyme substrate, a product of an enzyme catalyzed reaction, a nucleic acid, and a product of a nucleic acid catalyzed reaction, and wherein the component(s) of interest has not undergone chromatographic separation prior to step (iv).