Patent ID: 7229767
Filing Date: 2007-06-12
Classification: C12N,C12Q

Abstract:
1. A method for producing a stabilized double D loop at a target sequence within a double-stranded nucleic acid, the method comprising: providing a first oligonucleotide and a second oligonucleotide, said first and second oligonucleotides having at least a region of complementarity therebetween; wherein said first oligonucleotide is bound by a recombinase and has a region that is substantially complementary in sequence to a first strand of said target, and said second oligonucleotide is free of a recombinase and has a region that is substantially complementary in sequence to a second strand of said target; combining said first oligonucleotide with said double-stranded nucleic acid under conditions suitable for production of a double D-loop to form a mixture; and adding the second oligonucleotide to the mixture, producing-said stabilized double D loop at said target sequence within said double-stranded nucleic acid.