Patent ID: 6458568
Filing Date: 2002-10-01
Classification: C12P

Abstract:
A method for the production of baccatin-III by enzymatic acetylation of 10-deacetyl baccatin-III, comprising,(a) selectively aceylating 10-deacetyl baccatin-III at position C-10 to produce baccatin-III, by contacting 10-deacetyl baccatin-III with an acetyl transferase enzyme extracted from a Taxus species, in an aqueous reaction batch, wherein the aqueous reaction batch is separated from a composition comprising an organic solvent capable of extracting baccatin-III by a semipermeable membrane positioned therebetween, (b) extracting the baccatin-III produced by step (a) from the aqueous reaction batch into the organic solvent capable of extracting baccatin-III, wherein the semipermeable membrane selectively passes baccatin-III, and retains the acetyl transferase enzyme; (c) recovering the baccatin-III from the organic solvent; wherein said aqueous reaction batch comprises the acetyl transferase enzyme, 10-deacetyl baccatin-III, and acetyl coenzyme A.