Patent ID: 8231868
Filing Date: 2012-07-31
Classification: A61K,A61M,C12N

Abstract:
1. A method for suppressing the immune response of an individual receiving a transplantation of an organ or tissue, comprising the steps of: (a) treating an extracorporeal quantity of blood from the intended transplant recipient by causing the blood to flow through an apparatus having plastic channels to induce monocytes contained in the blood to differentiate into dendritic cells; (b) treating the dendritic cells to truncate maturation of the dendritic cells at a stage where the dendritic cells can induce suppression of an immune system response by treating the dendritic cells using a treatment selected from the group consisting of irradiation with gamma radiation, exposing the dendritic cells to mitomycin C, exposing the dendritic cells to cyclophosphamide, and exposing the dendritic cells to IL-10; (c) obtaining a quantity of blood from the intended transplant donor, wherein the blood contains a leukocyte cells from the intended transplant donor; (d) treating the blood containing leukocyte cells from the intended transplant donor to render the T cells contained in the donor's leukocyte cells apoptotic or necrotic; (e) combining the dendritic cells from the intended transplant recipient with the treated blood containing leukocyte cells from the intended transplant donor and incubating the combined cells; and (f) administering the incubated dendritic cells and the treated blood leukocyte cells to the intended transplant recipient.