Patent ID: 6239261
Filing Date: 2001-05-29
Classification: C07K

Abstract:
A process for the preparation of a and purified von Willebrand factor concentrate, which comprises:a) preparing a solution selected from the group consisting of cryoprecipitate, Cohn fraction I, a supernatant of a cell culture and an extract of a cell culture, said solution having a pH of 5.5 to 7.3, and containing von Willebrand factor (vWp) as a complex with F VIII:C, a carbohydrate at a concentration of 5-30% w/w, calcium ion, and amino acids;b) treating said solution with an anion exchanger to which F VIII:C binds to obtain a von Willebrand factor concentrate free of F VIII:C;c) treating the von Willebrand factor solution free of F VIII:C with 0.5 to 3 mol/l glycine to precipitate proteins from said solution;d) removing the protein precipitate from said solution to form a glycine supernatant solution containing von Willebrand factor;e) adding NaCl at a concentration of 2-15% w/v to said glycine supernatant solution to precipitate von Willebrand factor; andf) recovering precipitated von Willebrand factor.