Patent ID: 6620617
Filing Date: 2003-09-16
Classification: A61K

Abstract:
A method of transfecting cells in vitro in culture medium comprising contacting cells in a culture medium with a composition for delivery of naked DNA, said composition comprising:(a) a preparation of microparticles between 1 and 300 &mgr;m in diameter, each of which preparation of microparticles comprises a synthetic, biocompatible, biodegradable polymeric matrix; and (b) an effective amount of naked DNA dispersed within the preparation of microparticles, wherein said amount of naked DNA is greater than 20 &mgr;g, in which the DNA contains a gene operably linked to a promoter, the nucleotide sequence of said gene being greater than thirty nucleotides in length; wherein said DNA is released or diffused from said matrix over a period of at least three months.