Patent ID: 7303884
Filing Date: 2007-12-04
Classification: G01N

Abstract:
1. A method for indirectly determining blood clotting status INR (International Normalized Ratio) comprising the following steps: a) providing a sample of body fluid which contains a protein which can be modified by a vitamin K-dependent γ-carboxylase, wherein the body fluid is plasma, blood, saliva, or urine, b) measuring at least two concentrations selected from a group consisting of a first concentration (C1) of carboxylated protein, a second concentration (C2) of decarboxylated protein and a third concentration (C3) of total carboxylated and decarboxylated protein, where the first concentration (C1) is measured using a first antibody (A1), the second concentration is measured using a second antibody (A2) and the third concentration (C3) is measured using a third antibody (A3), c) obtaining a first ratio (R1) from the first (C1) and second concentration (C2) or forming a second ratio (R2) from the third (C3) and first concentration (C1) or forming a third ratio (R3) from the third (C3) and second concentration (C2), where a concentration (C1, C2, C3), which is necessary for forming the first (R1), second (R2) or third (R3) ratio, is not measured in step b) is calculated in accordance with the following relation: and d) correlating the first, second or third ratio (R1, R2, R3) with the blood clotting status.