Patent ID: 6740527
Filing Date: 2004-05-25
Classification: G01N,Y10S,Y10T

Abstract:
A multi-purpose, cyanide-free lyse reagent for making hemoglobin (Hb) and leukocyte (WBC) determinations comprising, in combination:(a) an aqueous multipurpose lyse reagent comprising at least one quaternary ammonium salt, said quaternary salt selected from the group consisting of: tetradecyltrimethyl ammonium bromide (TTAB), dodecyltrimethyl ammonium chloride, cetyl trimethyl ammonium bromide, hexadecyltrimethyl ammonium bromide, benzalkonium chloride, and cetylpyridium chloride; and (b) at least one hydroxylamine salt selected from the group consisting of: hydrochloride, sulfate, phosphate, and other acid salts; wherein said multipurpose lyse reagent produces a chromogen having maximum absorbance between 530 and 550 nm.