Patent ID: 6063607
Filing Date: 2000-05-16
Classification: C12N

Abstract:
An isolated polypeptide having choline oxidase activity obtained from Fusarium venenatum, selected from the group consisting of:(a) a polypeptide having an amino acid sequence which has at least 95% identity with amino acids 1 to 543 of SEQ ID NO.2;(b) a polypeptide which is encoded by a nucleic acid sequence which hybridizes under low stringency conditions with (i) nucleotides 49 to 1677 of SEQ ID NO. 1, (ii) a fragment of (i) of at least 100 contiguous nucleotides of nucleotides 49 to 1677 of SEQ ID NO. 1, or (iii) a complementary strand of (i) or (ii), wherein low stringency conditions are defined as prehybridization and hybridization at 42.degree. C. in 5.times. SSPE, 0.3% SDS, 200 .mu.g/ml sheared and denatured salmon sperm DNA, and 25% formamide, and washing with 2.times. SSC, 0.2% SDS at 50.degree. C.;(c) a polypeptide fragment of (a) or (b) that has choline oxidase activity.