Patent ID: 6605285
Filing Date: 2003-08-12
Classification: A61K,A61P

Abstract:
A process for the preparation of a vaccine from enterotoxin and cytotoxins isolated from Salmonella enterica subspecies for protection against Salmonellosis in poultry, comprising;treating the said toxins with formalin in a ratio appropriate to result in lost toxicity but retained immunogenicity and incubating for 40-50 hours at 35-38Â° C. temperature to form a formalized toxoid, removing formalin by dialysis against phosphate buffer saline (PBS), concentrating the formalized toxoid by dialysing against polyethylene glycol, and adding immuno-potentiator selected from Freund's Complete Adjuvant (FCA) or Vitamin E or Saponin, to said concentrated formalized toxoids to get the desired vaccine.