Patent ID: 6147190
Filing Date: 2000-11-14
Classification: A61K,A61P,C07K,C12N

Abstract:
An isolated protein encoded by a nucleic acid sequence capable of hybridizing to an E nucleic acid sequence (SEQ ID NO 163 or SEQ ID NO 137); wherein said hybridizing comprises the steps of:a) labelling said E nucleic acid;b) denaturing said E nucleic acid;c) adding said E nucleic acid to a hybridizaiion solution of 80% buffer B containing 10% dextran sulfate at 10.sup.6 dpm .sup.32 P per ml;d) adding said buffer B containing said E nucleic acid to a filter containing said nucleic acid encoding said polypeptide;e) incubating said filter for approximately 16 hours at 60.degree. C.;f) washing said filter at 60.degree. C. in buffer B for 15 minutes;g) washing said filter three times for 20 minutes in 2.times.SSC, 0.1% SDS; andh) washing said filter in 1.times.SSC, 0.1% SDS for 20 minutes.