Patent ID: 8206923
Filing Date: 2012-06-26
Classification: C12Q,Y02A

Abstract:
1. A method for detecting Listeria spp, Staphylococcus aureus, Campylobacter jejuni , and Escherichia coli O157:H7 in a food or environment sample, the method comprising the steps of: a) extracting DNA present in said food or environment sample; b) preparing a reaction mixture including said extracted DNA of said food or environment sample, a first pair of oligonucleotide primers consisting of an upstream primer of SEQ ID NO: 1 and a downstream primer of SEQ ID NO: 2, which anneals to a first target nucleic acid sequence of c) providing a thermostable DNA polymerase and a magnesium salt; d) amplifying by multiplex amplification reaction using real-time polymerase chain reaction (PCR), the first target nucleic acid sequence of e) following amplification, determining the presence or absence of the first, second, third and fourth target nucleic acids in the amplified PCR products by means of a fluorescent signal or fluorescence emission specific for each target nucleic acid; and wherein the Listeria spp, Staphylococcus aureus, Campylobacter jejuni and Escherichia coli O157:H7 are simultaneously detected and quantified in said food or environment sample.