Patent ID: 7364898
Filing Date: 2008-04-29
Classification: B01J,G01N

Abstract:
1. A method for a detection and quantification of a first set and a second set of target molecules upon at least one micro-array, said method comprising: providing a micro-array formed upon a solid support surface, comprising a first set and a second set of capture molecules, present in different locations of the solid support surface, wherein the first set of capture molecules is made of at least 3 capture molecules which are specific of the first set of target molecules and bind directly the first set of target molecules by specific molecular recognition, and wherein the second set of capture molecules is made of at least 3 capture molecules which are unrelated with the second set of target molecules and have no direct binding affinity with the second set of target molecules, the second set of capture molecules being capable of binding to adaptor molecules which bind to the second set of target molecules, contacting said first set of target molecules and said second set of target molecules with the first set of capture molecules and the second set of capture molecules, wherein said first set of target molecules and said second set of target molecules comprise at least one identical target molecule which is capable of binding to the fist set of capture molecules and to the adaptor molecules which bind to the second set of target molecules, wherein said contacting of said first set of target molecules with the first set of capture molecules is a direct assay, detecting specific signal(s) resulting from a binding of the target molecules to the first set and second set of capture molecules, and performing a relative quantification of the first set of target molecules compared to the second set of target molecules using a correction factor calculated from a ratio between a first signal resulting from the binding of said identical target molecule on the first set of capture molecules and a second signal resulting from the binding of the same identical target molecule on the second set of capture molecules.