Patent ID: 9068970
Filing Date: 2015-06-30
Classification: C12Q,G01N

Abstract:
1. A high-throughput method of screening cell culture media supplements to determine a concentration of a supplement combination capable of inducing an increase in peak viable cell density in a cell culture, the method comprising: a. supplementing a plurality of individual volumes of chemically defined media with a supplement combination comprising L-isoleucine (0.23-0.29 g/L); L-leucine (0.24-0.31 g/L); L-lysine (0.47-0.37 g/L); L-methionine (0.08-0.10 g/L); L-phenylalanine (0.15-0.19 g/L); L-threonine (0.21-0.26 g/L); L-tyrosine (0.15-0.19 g/L); L-valine (0.23-0.30 g/L); L-arginine (0.53-0.68 g/L); L-histidine*HCl*H b. inoculating the individual volumes with a cell line of interest; c. culturing the cell line of interest in the individual volumes; d. measuring peak viable cell density of the cultured cell line in the individual volumes; and e. performing Analysis of Variance statistical analysis of the measurements of peak viable cell density; wherein the results of the statistical analysis indicate the concentration of a supplement combination capable of inducing an increase in peak viable cell density in a cell culture.