Patent ID: 7547775
Filing Date: 2009-06-16
Classification: B01J,B82Y,C40B

Abstract:
1. A method of fabricating a plurality of oligonucleotides having free 3′-hydroxyl groups from a high density oligonucleotide array, said method comprising the steps of a) providing a solid substrate comprising a plurality of ribonucleotides attached thereto, one said ribonucleotide shown below wherein PG b) selectively removing PG c) reacting said free 3′-hydroxyl groups with a 2′-deoxyribonucleotide having the structure wherein PG d) selectively removing PG e) reacting said free 5′-hydroxyl groups with an additional 2′-deoxyribonucleotide having the structure to yield a product of the structure f) repeating steps d and e one or more times to provide said oligonucleotides attached to said solid substrate; g) removing PG h) transesterifying each of said one or more ribonucleotides to yield said solid substrate having a cyclic ester attached thereto and free oligonucleotides, each oligonucleotide having a 3′-hydroxyl group and having the structure