Patent ID: 8440390
Filing Date: 2013-05-14
Classification: A01N

Abstract:
1. A method for preserving a tissue, comprising: (i) immersing a tissue in a solution having a cryoprotectant concentration of at least about 75%, by weight relative to the weight of the solution; (ii) cooling the tissue in the solution having said cryoprotectant concentration of at least about 75% , by weight relative to the weight of the solution, to a temperature between about −20° C. and the glass transition temperature of the solution having said cryoprotectant concentration of at least about 75%, by weight relative to the weight of the solution, wherein the tissue is cooled and maintained a temperature above the glass transition temperature of the solution without ice formation; and (iii) after the tissue reaches the temperature between about −20° C. and the glass transition temperature of the solution having said cryoprotectant concentration of at least about 75% by weight, changing the concentration of the cryoprotectant in the solution in which the tissue was cooled in step (ii) to form a substantially cryoprotectant-free solution in a single step.