Patent ID: 6051402
Filing Date: 2000-04-18
Classification: A01K,A61K,A61P,C07K,C12N

Abstract:
An isolated nucleic acid molecule encoding an anti-sickling .beta.-globin protein having structure of human .beta.-globin and comprising the amino acid sequence shown in SEQ ID NO:1, wherein at least one amino acid residue of SEQ ID NO:1 has been substituted with an anti-sickling amino acid residue selected from the group consisting of: 9 Thr, 12 Asn, 22 Ala, 50 Ser, 80 Asp, 86 Ser, 87 Gln, 116 Arg, 117 Asn, 125 Gln and 126 Met, provided that said anti-sickling .beta.-globin protein does not contain all of said anti-sickling residues, wherein said anti-sickling .beta.-globin protein exhibits reduced sickling compared to wild-type .beta.-globin protein when present in a hemoglobin tetramer along with at least one .beta.6 Val mutated .beta.-globin protein.