Patent ID: 9200317
Filing Date: 2015-12-01
Classification: C12Q

Abstract:
1. A method for determining the presence and/or amount of a first polynucleic acid in a sample comprising subjecting the sample to nucleic acid amplification in which the presence and/or amount of the first nucleic acid is detectable by the presence of a reporter signal generated by polynucleic acid formation from the amplification of the first polynucleic acid, wherein the method comprises nucleic acid amplification of the first polynucleic acid and a second polynucleic acid in the same reaction vessel, the nucleic acid amplification being performed with a predetermined amount of the second polynucleic acid, wherein the product of the amplification of the first polynucleic acid and the product of the amplification of the second polynucleic acid are detected by the same reporter signal and wherein the reporter signals from amplification of the first polynucleic acid and the second polynucleic acid are resolved based on a set of readings of signal against time, the product of the second polynucleic acid amplification being produced with different reaction kinetics from the product of the amplification of the first polynucleic acid such that the second polynucleic acid acts as an internal control for the method.