Patent ID: 8993840
Filing Date: 2015-03-31
Classification: C07K,C12N,C12Y

Abstract:
1. A soybean plant seed with increased oil content comprising in its genome: (a) a first recombinant DNA construct comprising a first polynucleotide operably linked to at least one first regulatory element, wherein said first polynucleotide comprises a diacylglycerol acyltransferase (DGAT) coding region encoding a polypeptide having at least 95% sequence identity to SEQ ID NO:180, based on the Clustal W method of alignment with pairwise alignment default parameters of KTUPLE=1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5, wherein said DGAT polypeptide has DGAT activity; (b) a second recombinant DNA construct comprising a second polynucleotide operably linked to at least one second regulatory element, wherein said second polynucleotide encodes a SUT4 sucrose transporter polypeptide, wherein said SUT4 sucrose transporter polypeptide has at least 95% sequence identity to SEQ ID NO:38, based on the Clustal W method of alignment with pairwise alignment default parameters of KTUPLE=1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5, and wherein said SUT4 sucrose transporter polypeptide has sucrose transport activity; and (c) wherein said soybean plant seed exhibits increased oil content as a percentage of seed weight when compared to a control soybean plant seed comprising said first recombinant DNA construct but not comprising said second recombinant DNA construct.