Patent ID: 6063629
Filing Date: 2000-05-16
Classification: C12M,C12N

Abstract:
A microinjection process for introducing an injection substance into procaryotic or eucaryotic cells, or cell components of the latter by means of a nanopipette and pressure, comprising filling a nanopipette, which has an external diameter of 0.5 to 2 mm, an internal diameter of 0.1 to 1.5 mm and a tip diameter of 0.025 to 0.3 .mu.m, with an injection substance and a heat-expandable substance or substance mixture, sealing a capillary of the nanopipette with an adhesive, sticking the nanopipette tip with the aid of a microscope and a micromanipulator into the cell or cell compartment, and heating the nanopipette by means of an adjustable heater until the injection substance passes out of the nanopipette tip at an outflow rate of up to 1 femtoliter per second and enters the cell or cell compartment.