Patent ID: 6383748
Filing Date: 2002-05-07
Classification: B01F,B01J,B01L,G01N,Y10S

Abstract:
A method for analyzing one or more fluid samples for the presence, amount or identity of one or more analytes present in said samples, comprising the steps of:(a) providing a device comprising one or more round wells with a fixed diameter, said wells exposing a substrate having a thickness, said substrate having oriented through going channels, and an area of the substrate exposed in the well being provided with at least one binding substance specific for at least one of said analytes; (b) adding an amount of sample fluid to one or more of the wells of the device; (c) generating an alternating flow through the substrate in the wells by applying an alternating pressure difference over the substrate in the wells, said alternating pressure difference being lower than a capillary pressure of the channels in the substrate, whereby a liquid volume of the sample is forced to pass through the channels in the substrate from an upper side of the substrate to a lower side of the substrate and back at least one time, so as to allow for a reaction between analyte present in the sample and the binding substance(s); (d) reading any signal generated in any of the wells; and (e) determining from said signal(s) the presence, amount and/or identity of said one or more analytes.