Patent ID: 6972013
Filing Date: 2005-12-06
Classification: A61N

Abstract:
1. An in vivo method for enhancing expression of a therapeutic polypeptide encoded by an isolated polynucleotide to be delivered into skin cells in vivo in a subject, said method comprising: a) introducing about 0.05 μg to about 100 μg per skin tissue site of at least one isolated polynucleotide encoding a therapeutic polypeptide into one or more skin tissue sites of a subject; b) applying at least two non-invasive electrodes to the skin tissue site; c) generating a total charge transfer at the skin tissue site of about one mCoulomb (mC) to about 1000 mC per skin tissue site by introducing one or more electric pulses to generate said total charge transfer at the skin tissue site, at substantially the same time as the introduction of the polynucleotide so as to result in the polynucleotide entering cells of the skin tissue for expression of the therapeutic polypeptide therein; thereby enhancing the expression of the therapeutic polypeptide as compared to expression of the therapeutic polypeptide achieved by other methods for generating an electric field in target skin tissue.