Patent ID: 6010887
Filing Date: 2000-01-04
Classification: C12N

Abstract:
A method of regulating a target gene in a target eukaryotic genome by pseudo-operator/repressor interaction comprising:(a) identifying said pseudo-operator as a DNA sequence which is upstream of said target gene and has overall dyad symmetry of an operator;(b) transforming a bacterial host with a recombinant plasmid which contains(i) said pseudo-operator operatively linked to a marker gene and controlling expression thereof, and(ii) a gene encoding a potential bacterial repressor for said pseudo-operator;(c) selecting a culture of transformants, wherein said repressor is identified by repression of marker gene expression in the bacterial host by positive selection for said repression of marker gene expression; and(d) transforming said target eukaryotic genome with a gene encoding said selected repressor, wherein said selected repressor gene is capable of regulating said target gene.