Patent ID: 6521417
Filing Date: 2003-02-18
Classification: A01K,A61K,A61P,C07K,C12N,G01N

Abstract:
A method of identifying protein substrates for a mutant protein kinase comprising incubating permeabilized cells expressing the mutant protein kinase with a radiolabeled analog for an appropriate amount of time, lysing the cells, separating the lysate by SDS-PAGE, and identifying the protein substrates,wherein said analog is a gamma-P32 radiolabeled N6-substituted ATP analog that is a substrate for a mutant form of a wild-type protein kinase, wherein the analog does not serve as a substrate for the wild-type protein kinase; and, wherein said mutant protein kinase is selected from the group consisting of: a mutant protein kinase which comprises an alanine or a glycine as the amino acid which corresponds to position 21 of SEQ ID NO: 3, which is position 338 of v-Src; and, a mutant protein kinase which comprises an alanine or a glycine as the amino acid which corresponds to position 21 of SEQ ID NO: 3, which is position 338 of v-Src and which further comprises an alanine or glycine as the amino acid which corresponds to position 6 of SEQ ID NO. 3 which is position 323 of v-Src.