Patent ID: 8951726
Filing Date: 2015-02-10
Classification: C12Q

Abstract:
1. A method of detecting two or more nucleic acids of interest, the method comprising: a) providing a sample comprising or suspected of comprising the two or more nucleic acids of interest; b) capturing the nucleic acids of interest present in the sample on a solid support, wherein the solid support comprises a plurality of capture probes, and wherein first and second nucleic acids of interest are captured on the solid support by hybridizing two or more capture extenders to each of the first and second nucleic acids and to two or more of the capture probes; c) providing two or more subsets of m label extenders, wherein m is at least two, wherein a first subset of m label extenders is capable of hybridizing to the first nucleic acid of the nucleic acids of interest and the second subset of m label extenders is capable of hybridizing to a second nucleic acid of the nucleic acids of interest, thereby providing at least two subsets of m label extenders capable of hybridizing to at least two nucleic acids of interest, wherein each label extender comprises a polynucleotide sequence L-1 that is complementary to a polynucleotide sequence in the corresponding nucleic acid of interest and a polynucleotide sequence L-2 that is complementary to a polynucleotide sequence in the component of the label probe system; and wherein the m label extenders in a subset each have L-1 5′ of L-2 or wherein the m label extenders in a subset each have L-1 3′ of L-2; d) providing two or more label probe systems, each label probe system comprising a label, wherein a component of the first label probe system is capable of hybridizing simultaneously to at least two of the m label extenders in the first subset of m label extenders and a component of the second label probe system is capable of hybridizing simultaneously to at least two of the m label extenders in the second subset of m label extenders; e) hybridizing each nucleic acid of interest captured on the solid support to its corresponding subset of m label extenders; f) hybridizing the two or more label probe systems to their corresponding subset of m label extenders; and g) detecting the presence or absence of the two or more label probe systems on the solid support.