Patent ID: 6399336
Filing Date: 2002-06-04
Classification: C12N,C12P

Abstract:
A large-scale method of sialylating a saccharide group on a recombinant glycoprotein, the method comprising contacting a saccharide group which comprises a galactose or N-acetylgalactosamine acceptor moiety on a recombinant glycoprotein with a sialic acid donor moiety and a recombinant sialyltransferase in a reaction mixture which provides reactants required for sialyltransferase activity for a sufficient time and under appropriate conditions to transfer sialic acid from said sialic acid donor moiety to said saccharide group wherein at least about 80% of the saccharide groups are sialylated, and wherein said recombinant sialyltransferase is present at 50 mU or less per mg of said recombinant glycoprotein.