Patent ID: 6669947
Filing Date: 2003-12-30
Classification: A61K,A61P

Abstract:
A method for preparing a composition comprising at least one antigenic phase, at least one adjuvant phase, and optionally a diluent phase, wherein the at least one antigenic phase and the at least one adjuvant each comprise one or more phases which are distinct from each other when the composition is in a solid state, and the composition is in the liquid state when its temperature is greater than or equal to 4Â° C., said method comprising the steps of:a) bringing a first of an adjuvant, diluent or antigenic phase, which is liquid at room temperature, to a temperature of less than or equal to its solidification point so as to form a first solid phase, b) adding a second antigenic, adjuvant or diluent phase, in the liquid state, over the solid phase prepared in a), and then bringing the new combination to a temperature of less than or equal to the lowest solidification point of the two phases so as to form a solid combination with two distinct phases, c) adding, where appropriate, a new antigenic, adjuvant or diluent phase, in the liquid state, over said solid combination prepared in step b), and then bringing the new combination to a temperature of less than or equal to the lowest solidification point of the three phases so as to form a solid combination with three distinct phases, and d) repeating the sequence of operations carried out in step c), where appropriate, until the last of the antigenic, adjuvant or diluent phases comprising said composition has been frozen wherein the frozen composition comprises at least one antigenic phase, at least one adjuvant phase, and optionally at least one diluent phase.