Patent ID: 7718403
Filing Date: 2010-05-18
Classification: C12N,C12Q,C40B

Abstract:
1. A method of preparing a plurality of nucleic acid molecules having known constant region at each end, comprising: a) obtaining a sample comprising nucleic acid molecules; b) subjecting said nucleic acid molecules to a population of primers to form a nucleic acid molecule/primer mixture, wherein the primers of the population are non-self-complementary and non-complementary to other primers in the population, and comprise in a 5′ to 3′ orientation a constant region and a variable region, wherein the constant region sequence has a known sequence that is constant among a plurality of primers of the population and the variable region sequence is degenerate among said plurality of primers of the population, and further wherein the sequence of the constant and variable regions consists essentially of only two types of non-complementary nucleotides selected from the group consisting of adenines and guanines; adenines and cytosines; guanines and thymidines; and cytosines and thymidines, such that the primers of the population will not cross-hybridize or self-hybridize under the conditions employed in step c); and c) subjecting said nucleic acid molecule/primer mixture to a polymerase under isothermal conditions to generate the plurality of molecules including the known constant region at each end.