Patent ID: 7534934
Filing Date: 2009-05-19
Classification: C07K,C12N

Abstract:
1. A method for producing a plant, comprising (1) transforming a plant cell using Agrobacterium with a desired polynucleotide, wherein the desired polynucleotide is flanked by at least one sequence of (a) 25 nucleotides in length that (b) promotes and facilitates integration of the desired polynucleotide into the plant genome and which (c) is not 100% identical to a T-DNA border, and wherein (d) the 25 nucleotide-long sequence comprises a plant DNA sequence that comprises the consensus nucleotide sequence of SEQ ID NO:93 (ANGATNTATN 6 GT), where “N” is an A, G, C, or T nucleotide; and (2) growing a plant from said transformed plant cell, which comprises in its genome the desired polynucleotide.