Patent ID: 6683159
Filing Date: 2004-01-27
Classification: C07K

Abstract:
A method for purification of a Factor VIII polypeptide comprising:(a) adding a mixture containing a Factor VIII polypeptide to be purified to an immunoaffinity matrix which binds the Factor VIII polypeptide by hydrophobic attraction; (b) eluting the Factor VIII polypeptide from the immunoaffinity matrix with an elution solution which desorbs the Factor VIII polypeptide; (c) diluting the elution solution about 3-fold to about 5-fold with a solution comprising higher NaCl concentration than that of the elution solution, resulting in a diluted Factor VIII solution comprising about 7-20 mM NaCl; (d) passing the diluted Factor VIII solution through an ion exchange column which binds the Factor VIII polypeptide, thereby allowing contaminants to pass through the ion exchange column; and (e) eluting the purified factor VIII polypeptide from the ion exchange column.