Patent ID: 7932023
Filing Date: 2011-04-26
Classification: C07K,G01N

Abstract:
1. A method of detecting a cancer specific proliferating cell nuclear antigen (csPCNA) isoform in a biological sample, the method comprising: detecting a posttranslational modification comprising a methyl ester on one or more of 16 aspartic acid or glutamic acid residues corresponding to the amino acid positions 3, 85, 93, 94, 104, 109, 115, 120, 132, 143, 174, 189, 201, 238, 256, and 259 of SEQ ID NO: 30 in the sample suspected of containing the csPCNA isoform; and comparing the levels of methyl esters on the csPCNA isoform with a nonmalignant isoform of PCNA, wherein the csPCNA isoform has a lower level of methyl esterification than the nonmalignant isoform of PCNA.