Patent ID: 6649395
Filing Date: 2003-11-18
Classification: C07K,C12N,Y10S

Abstract:
A method of screening for compounds capable of inhibiting peptidyl-prolyl cis-trans isomerase (PPIase) activity of a tyrosine-containing cyclophilin, or functional moiety thereof, the cyclophilin of the type identified in C. elegans, O. volvulus, B. malayi or D. immitis in which the conserved tryptophan at the CsA drug binding domain has been substituted by a tyrosine and where the cyclophilin is not inhibited by cyclosporin A, comprising:(a) contacting the tyrosine-containing cyclophilin with a test compound; (b) measuring an effect of the test compound on PPIase activity of the tyrosine-containing cyclophilin; and (c) determining whether the test compound is an inhibitor of the PPIase activity of the tyrosine-containing cyclophilin.