Patent ID: 6136571
Filing Date: 2000-10-24
Classification: C12N,C12P

Abstract:
A method of producing one or more monosaccharides and oligosaccharides, comprising the steps of:(a) subjecting a liquefied starch solution to saccharification which comprises more than one enzymatic saccharification stage;(b) subjecting the saccharification liquor from one of the saccharification stages to a microfiltration step;(c) subjecting the permeate of the microfiltration step to a nanofiltration step;(d) re-circulating the retentate from the nanofiltration step to a saccharification stage of step (a), at which saccharification stage the content of the reaction mixture matches the content of the retentate with respect to the mono or oligosaccharide, and(e) recovering the permeate of the nanofiltration step which comprises one or more monosaccharides and oligosaccharides.