Patent ID: 8017314
Filing Date: 2011-09-13
Classification: C12Q

Abstract:
1. A method for measuring the concentration of at least one analyte with the aid of an analyte-specific oxidase, said method comprising: providing an immersion sensor, said immersion sensor comprising at least one enzyme region with the analyte-specific oxidase immobilized therein, two electrodes lying in parallel and terminating in the at least one enzyme region, at least one space for gaseous oxygen formed in between and bordering the electrodes and bordering the at least one enzyme region directly or via an oxygen-permeable material, and at least one channel leading from the at least one enzyme region to a surface of the immersion sensor which limits diffusion and contains water; introducing said at least one analyte into said immersion sensor, wherein said at least one analyte diffuses into said at least one enzyme region via said at least one channel and wherein said at least one analyte reacts with said analyte-specific oxidase, consuming oxygen and producing volatile hydrogen peroxide; measuring the concentration of the at least one analyte by measuring amperometrically the reaction of the at least one analyte with said analyte-specific oxidase with the immersion sensor; and correlating the concentration of the at least one analyte with said hydrogen peroxide production.