Patent ID: 8586826
Filing Date: 2013-11-19
Classification: C12N

Abstract:
1. A polynucleotide comprising a tobamoviral nucleotide sequence, the tobamoviral nucleotide sequence comprising: a first nucleotide sequence encoding a tobamoviral replication protein; a second nucleotide sequence encoding a tobamoviral movement protein, the second nucleotide sequence being located downstream of the first nucleotide sequence; and a linking site for linking with an exogenous nucleotide sequence encoding a polypeptide to be expressed, wherein the linking site is located downstream of the second nucleotide sequence, and wherein the second nucleotide sequence comprises a mutation relative to the wild-type nucleotide sequence encoding the tobamoviral movement protein, wherein the mutation comprises an insertion of 300 or more contiguous nucleotides, and wherein the insertion is a sequence other than an intron.