Patent ID: 6156300
Filing Date: 2000-12-05
Classification: A61K,C07K,C12N

Abstract:
A CSF-1 carboxy truncated polypeptide consisting of:(a) a CSF-1 active carboxy truncated polypeptide containing at least amino acid residues 3 to 190 of SEQ ID NO: 2 and being selected from the group consisting of N.gradient.2C.gradient.190 CSF-1, N.gradient.2C.gradient.191 CSF-1, N.gradient.2C.gradient.221 CSF-1, N.gradient.2C.gradient.223 CSF-1, N.gradient.2C.gradient.236 CSF-1, N.gradient.2C.gradient.238 CSF-1, N.gradient.2C.gradient.249 CSF-1, N.gradient.2C.gradient.250 CSF-1, N.gradient.2C.gradient.258 CSF-1 and N.gradient.2C.gradient.411 CSF-1; andwherein in said CSF-1 carboxy truncated polypeptide at least one of the amino acids at positions 52, 59, 150, 158 or 159 is substituted with Gln/Pro, Asp/Tyr, Gly, Pro or Asp/Pro, respectively; orwherein at least one of (i) an Asp at any of residue positions 122, 140, 349, or 383, or (ii) a Thr at residue positions 124 or 351, or (iii) a Ser at residue positions 142 or 385, is replaced with an amino acid that will inactivate the glycosylation site; or(b) a CSF-1 active carboxy truncated polypeptide containing at least residues 3 to 190 of SEQ ID NO: 4 and being selected from the group consisting of N.gradient.2C.gradient.190 CSF-1, N.gradient.2C.gradient.191 CSF-1, N.gradient.2C.gradient.221 CSF-1 and N.gradient.2C.gradient.223 CSF-1; andwherein in said CSF-1 carboxy truncated polypeptide at least one of the amino acids at positions 52, 59, 158, 159 is substituted with Gln/Pro, Asp/Tyr or Asp/Pro or Pro, respectively; orwherein at least one of (i) an Asp at any one of residue positions 122 or 140, or (ii) a Thr at residue position 124 or (iii) a Ser at residue position 142 is replaced with an amino acid that will inactivate the glycosylation site.