Patent ID: 8357797
Filing Date: 2013-01-22
Classification: C07D,C12Q,G01N

Abstract:
1. A method of forming a 3-phenylimino-3H-phenoxazine mediator, the method comprising the acts of: providing a first reactant including phenoxazine; providing a first solvent in which the phenoxazine has a desired solubility therein; providing a second reactant to assist in forming the 3-phenylimino-3H-phenoxazine mediator; providing a second solvent in which the second reactant has a desired solubility therein; combining the first reactant, first solvent, second reactant and second solvent to form a reactants solution; adding sodium persulfate to the reactants solution to couple the first and second reactants resulting in a reaction solution including the 3-phenylimino-3H-phenoxazine mediator; and after adding the sodium persulfate, further processing to the reaction solution including the 3-phenylimino-3H-phenoxazine mediator so as to isolate the 3-phenylimino-3H-phenoxazine mediator.