Patent ID: 8303998
Filing Date: 2012-11-06
Classification: A61K,A61P,B01J,C07H

Abstract:
1. A process for obtaining a glucosinolate-rich eluate from broccoli seeds in an amount sufficient for production of food-grade or nutraceutical-grade glucosinolates, the process consisting essentially of: a) extracting a glucosinolate-containing extract from broccoli seeds with an extraction medium that comprises a lower alcohol or ketone or a mixture thereof with water, to obtain an alcoholic or ketonic glucosinolate-containing extract; b) contacting the glucosinolate-containing extract of step a) with a cation ion-exchange resin of a cationic ion-exchange column; c) adsorbing the glucosinolate-containing extract from step b) onto a basic resin of a basic ion-exchange column to obtain an adsorbed glucosinolate-containing extract; and d) eluting the adsorbed glucosinolate-containing extract of step c) by adding sufficient base to increase the pH to at least 7.0 to form glucosinolate-containing salts having alkali- or earth-alkali cations, and separating the resulting glucosinolate-containing salts to obtain the glucosinolate-rich eluate from broccoli seeds.