Patent ID: 9187783
Filing Date: 2015-11-17
Classification: B01L,B03C,C12Q,C40B,G01N,H01L

Abstract:
1. A method for sequencing, comprising: (a) providing a plurality of particles, wherein each particle of said plurality is coupled to on average at most one nucleic acid molecule; (b) performing simultaneous nucleic acid amplification reactions on said nucleic acid molecules coupled to said particles, wherein said nucleic acid amplification reactions are performed while subjecting the particles to an electric field, thereby producing isolated, clonally-amplified nucleic acid molecules coupled to said plurality of particles; (c) performing nucleic acid extension reactions on said clonally-amplified nucleic acid molecules using primers that hybridize to said clonally-amplified nucleic acid molecules; and (d) detecting nucleic acid incorporation events from said extension reactions using a sensor array to determine a sequence of each of said clonally-amplified nucleic acid molecules, wherein each particle of said plurality of particles is adjacent to a sensor of said sensor array, and wherein each said sensor of said sensor array comprises two electrodes that are within a Debye length of their respective individual particle or within a Debye length of a nucleic acid molecule on said individual particle.