Patent ID: 7723507
Filing Date: 2010-05-25
Classification: C12Q

Abstract:
1. A kit for identifying a dog as normal, heterozygous for, or homozygous for a Collie Eye Anomaly mutation in a region of chromosome 37, wherein the mutation consists of a deletion of nucleotides 9,302 to 17,101 of SEQ ID NO:1, comprising: a set of primers, wherein the only primers in the kit are a first primer, a second primer, a third primer and a fourth primer, wherein the first primer, the second primer, the third primer, and the fourth primer each consist of a fragment of SEQ ID NO:1 or the complement thereof, wherein the first primer binds to a sequence upstream of position 9,301 of SEQ ID NO:1, the fourth primer binds to a sequence downstream of position 17,102 of SEQ ID NO:1 and second and third primers binds to a sequence between nucleotides 9,302 and 17,101 of SEQ ID NO:1, wherein amplification of an allele that does not comprise the mutation with the first and the second primers produces a first amplification product that comprises the nucleotide 9,302 of SEQ ID NO:1, amplification of an allele that does not comprise the mutation using the third and fourth primers produces a second amplification product that comprises the nucleotide 17,101 of SEQ ID NO:1, and amplification using the first and the fourth primer produces a third amplification product, wherein the third amplification product comprises SEQ ID NO:14 or a fragment thereof wherein the fragment thereof comprises contiguous nucleotides 89 and 90 of SEQ ID NO:14.