Patent ID: 6514714
Filing Date: 2003-02-04
Classification: G01N,Y10S

Abstract:
A method for determining the percentage of panel reactive antibodies in serum of a subject against human leukocyte antigens (HLA) antigens, said method comprising:providing a collection of microbeads of different subtypes, wherein each subtype is coated with different purified HLA antigens, and the microbeads of at least one subtype each present HLA antigens derived from a cell population presenting the same HLA antigens; adding said serum from said subject to said collection of microbeads; incubating said serum and microbeads for sufficient time for anti-HLA antibodies in said serum to bind to said HLA antigens; incubating said microbeads with at least one labeled ligand capable of specifically binding with said anti-HLA antibodies bound to said HLA antigens; detecting the presence of labeled ligand bound to said anti-HLA antibodies by flow cytometry to determine the presence or absence of panel reactive antibodies; and, determining the percentage of panel reactive antibodies.