Patent ID: 8574871
Filing Date: 2013-11-05
Classification: C07K,C12N,C12P

Abstract:
1. Genetically modified yeast cells, capable of producing glycoproteins having homogeneous glycans comprising the Man5GlcNac2 structure, said yeast cells comprising the following modifications: a) inactivation of the Och1 gene coding for α1,6-mannosyl transferase by insertion by homologous recombination of a heterologous sequence coding for a gene of resistance to an antibiotic (delta-Och1 strain); b) integration by homologous recombination into an auxotrophy marker of an expression cassette comprising a promoter selected from pGAP, pGAL1, PGK, TEF, adh1, nmt 1, SV40, PMA1, CaMV, pet56 of c) integration by homologous recombination into an auxotrophy marker of an expression cassette comprising a promoter selected from pGAP, pGAL1, PGK, TEF, adh1, nmt 1, SV40, PMA1, CaMV, pet56 of wherein the yeast cells are