Patent ID: 7494767
Filing Date: 2009-02-24
Classification: A61K,A61P,C07K,C12N,G01N

Abstract:
1. A method for identifying a peptide or fragment derived from a mammalian tumor susceptibility gene 101 (Tsg101) protein, wherein the peptide or fragment is effective in reducing HIV particle production, said method comprising: (a) introducing an expression construct into a mammalian cell or cells, wherein the expression construct comprises a portion of a mammalian tsg101 gene; (b) introducing one or more expression constructs into the mammalian cell or cells, wherein the one or more expression constructs comprise the HIV gag, pol, and rev gene sequences; (c) incubating the transfected mammalian cell or cells in a suitable media for a sufficient time and at a temperature of about 37° C. to obtain a mammalian cell culture comprising mammalian cells which express a mammalian Tsg101 peptide or fragment and HIV Gag, Pol and Rev proteins; (d) measuring the level of particle-associated p24 in the mammalian cell culture; and (e) correlating a reduced level of particle-associated p24, when compared to a control mammalian cell culture which has not been transfected with the expression construct comprising a portion of a mammalian tsg101 gene, with the identification of a peptide or fragment effective in reducing HIV particle production.