Patent ID: 8574905
Filing Date: 2013-11-05
Classification: C12N

Abstract:
1. A method of enriching a population of liver precursor cells, said method comprising: providing a cell population comprising human pluripotent cells with a growth factor of the TGFβ superfamily; allowing sufficient time for at least a portion of said human pluripotent cell population to differentiate into definitive endoderm cells; providing said cell population comprising definitive endoderm cells with BMP4 and FGF10; allowing sufficient time for at least a portion of said definitive endoderm cells to differentiate into liver precursor cells; contacting said cell population comprising liver precursor cells with a reagent that binds to a marker selected from the group consisting of DPP4, NOSTRIN, DOK4, SLC35D1, OCDH17, FN1, HLA-C, HLA-B, CYP4X1, CPHA6, PP1057 HPX and DGKB; and separating cells bound to said reagent from cells that are not bound to said reagent, thereby producing an enriched population of liver precursor cells.