Patent ID: 6241701
Filing Date: 2001-06-05
Classification: A61N,C12M

Abstract:
An electroporation device, comprising:(a) an electrode applicator having at least two opposing pairs of electrode needles arranged relative to one another to form a desired array;(b) an applicator handle having a first end detachably engaged to the electrode applicator and a second end positioned to receive at least one electrical signal from a power supply, the applicator handle configured to supply the at least one electrical signal to the electrode applicator, and(c) a power supply to provide at least one electrical signal to the second end of the applicator handle configured to supply the signal to the electrode applicator;wherein, when selected molecules are provided to a biological sample comprising cells and at least two in pairs of electrodes are in electrical contact with the biological sample and are activated simultaneously, the electrode applicator is operable to provide separately applied pulses of high amplitude electric signals to said at least two opposing pairs of electrodes, but not all electrodes if there are more than two opposing pairs of electrodes, simultaneously and proportionately to the distance between said electrodes of a pair to electroporate the selected molecules into cells of the sample without permanently damaging the cells.