Patent ID: 6458581
Filing Date: 2002-10-01
Classification: A61K,C07K,C12N,Y02A

Abstract:
A method for the in vitro culture of different stages of tissue parasites, consisting of culturing said parasites in a totally defined culture medium, which is an axenic, monophasic liquid culture medium, which is devoid of serum, and for obtaining amastigote forms, is buffered at a pH of 5.5 to 6.5 and has an osmolarity of at least 400 milliosmoles/kg of liquid, or, for obtaining promastigote forms, is buffered at a pH of 7 to 7.5 and has an osmolarity of at least 300 milliosmoles/kg of liquid,wherein said culture medium contains a basic culture medium for insect cells and at least one of an inorganic salt, a source of amino acids, and a sugar.