Patent ID: 6833489
Filing Date: 2004-12-21
Classification: A01K,C12N,G01N

Abstract:
A method for evaluating a test compound's ability to inhibit prolyl-4-hydroxylase (P4H), comprising the steps of:(a) introducing a test compound into a test chimeric Caenorhabditis elegans, a P4H-gene modified Caenorhabditis elegans, or a wild-type Caenorhabditis elegans, wherein the test chimeric Caenorhabditis elegans comprises a P4H gene that complements an endogenous P4H gene mutation, wherein the mutation results in an endogenous P4H that is not functional, and (b) observing the effect of the test compound on the prolyl 4-hydroxylase activity of the progeny of the test nematode, P4H-gene modified nematode or the wild-type nematode and determining that the effect of the test compound is due to its effect on prolyl-4-hydroxylase activity, wherein a dpy or embryonic lethal phenotype indicates prolyl-4-hydroxylase inhibition.