Patent ID: 7700356
Filing Date: 2010-04-20
Classification: A01K,C12N

Abstract:
1. A method for targeting a heritable integration of a transgene within a genome of a somatic or germ line cell of an invertebrate organism, said method comprising: integrating into said genome a first DNA cassette carrying a first transposon half side at one end, a second transposon half side in opposite orientation at the other end, a first wild-type, non-mutated recombinase target site, and a second, mutated recombinase target site that is heterospecific to the first recombinase target site, wherein the recombinase target sites are flanked by said transposon half sides, catalyzing an exchange between said first DNA cassette for a second DNA cassette by introducing into the cell comprising said genome a site-specific recombinase that catalyzes a DNA recombination reaction between homospecific recombinase target sites and said second DNA cassette, said second DNA cassette comprising the same first, wild-type, non-mutated and second, mutated recombinase target sites in the same orientation as those of the first DNA cassette, an internal transposon half side flanked by said recombinase target sites, said internal half side being in the same orientation as the second transposon half side of the first DNA cassette, and the transgene positioned between said internal half side and the second, mutated recombinase site, to result in integration of the transgene into the genome; and identifying that said transgene has been integrated into said genome.