Patent ID: 6087115
Filing Date: 2000-07-11
Classification: C07K

Abstract:
A method of identifying a negative antagonist of a constitutively active G protein coupled receptor of human herpesvirus 8, said method comprising:co-expressing in a host cell a constitutively active G protein coupled receptor of human herpesvirus 8 and a reporter protein, wherein expression of the reporter protein is controlled by a promoter responsive to a signaling pathway activated by the constitutively active G protein coupled receptor of human herpesvirus 8;determining a first activity level of the reporter protein;exposing the host cell to a test substance;determining a second activity level of the reporter protein after said exposing the host cell to the test substance; andidentifying the test substance, where the second activity level is less than the first activity level, as a negative antagonist of the constitutively active G protein coupled receptor of human herpesvirus 8.