Patent ID: 6150134
Filing Date: 2000-11-21
Classification: A61K,A61P,C07K,C12N,Y10S

Abstract:
A recombinant HCV single or specifically oligomerized envelope viral protein selected from the group consisting of E1, E2 or E1/E2 viral proteins obtained from a method comprising the steps of(a) optionally lysing host cells expressing said viral protein;(b) optionally recovering said viral protein;(c) cleaving disulphide bonds of said viral protein with a disulphide bond cleaving agent to form a cleaved protein;(d) preventing disulphide bond reformation of said cleaved viral protein with at least one of an SH group blocking agent and low pH condition; and(e) purifying the cleaved viral protein obtained in step (d) to produce a viral protein which is at least 80% purified.