Patent ID: 8852576
Filing Date: 2014-10-07
Classification: A61P,C07K

Abstract:
1. A method for increasing a substrate of a sodium iodide symporter protein within a cell in vitro, comprising: a) introducing a modified sodium iodide symporter protein into a cell; and b) contacting the cell with one or more substrates of a sodium iodide symporter protein; wherein the modified sodium iodide symporter protein comprises the amino acid sequence of SEQ ID NO. 1 with the proviso that at least one of the following conditions is satisfied: (1) residue glutamine (Q) 218 is replaced by arginine (R); (2) residue threonine (T) 308 is replaced by lysine (K); and residue serine (S) 319 is replaced by aspartic acid (D); and expression of the modified protein in a cell results in higher intracellular levels of a substrate of a sodium iodide symporter protein than does expression of the same amount of a wild-type sodium iodide symporter protein; and the modified sodium iodide symporter protein is introduced into the cell by the following steps: a1) introducing an expression vector comprising a polynucleotide encoding the modified sodium iodide symporter protein into the cell; and a2) culturing the cell to express the modified sodium iodide symporter protein, wherein, the polynucleotide comprises a nucleic acid sequence of SEQ ID NO.2, SEQ ID NO.3, SEQ ID NO.4, SEQ ID NO.5, SEQ ID NO.6, SEQ ID NO.7, or SEQ ID NO.8.