Patent ID: 6090557
Filing Date: 2000-07-18
Classification: C12N,C12Q,Y10S

Abstract:
A Neisseria gonorrhoeae-specific oligonucleotide, wherein said oligonucleotide consists of a nucleotide sequence between about 15 and about 50 nucleotides in length substantially complementary to a Neisseria gonorrhoeae cytosine DNA methyltransferase gene that is SEQ ID NO: 1 in a region which encompasses a nucleotide position selected from nucleotide positions numbers 618, 621, 627, 630, 636, 653, 654, 669, 678, 681, 683, and 702; wherein said sequence is exactly complementary to SEQ ID NO: 1 at each nucleotide position selected from nucleotide positions numbers 618, 621, 627, 630, 636, 653, 654, 669, 678, 681, 683, and 702 encompassed within said region, and wherein said Neisseria gonorrhoeae-specific oligonucleotide is capable of distinguishing by selective hybridization said Neisseria gonorrhoeae cytosine DNA methyltransferase gene from cytosine DNA methyltransferase genes from Neisseria strains that differ in nucleotide sequence from SEQ ID NO: 1 at nucleotide position numbers 618, 621, 627, 630, 636, 653, 654, 669, 678, 681, 683, and 702.