Patent ID: 6900041
Filing Date: 2005-05-31
Classification: A61P,C12Q,G01N

Abstract:
1. A method for detecting a composition that effects the activity or expression of glycogen synthase kinase 3 beta (GSK-3β), comprising, a) adding an inflammatory molecule to a first cell culture and a second cell culture, wherein the first cell culture and the second cell culture contain the same type of cells; b) adding a composition comprising a test compound suspected of effecting the activity or expression of GSK-3β to the first cell culture; c) measuring the amount of a determinant of GSK-3β in the first cell culture and the second cell culture, wherein the determinant is a cellular factor which is vascular cell adhesion molecule 1 (VCAM-1), monocyte chemoattractant protein-1 (MCP-1), interleukin-6 (IL-6), interferon-inducible protein (IP-10), monokine induced by IFN-γ (MIG), or interferon-inducible T-cell α chemoattractant (1-TAC); and d) comparing the amount of the determinant of GSK-3β in the first cell culture to the amount of determinant in the second cell culture, wherein a difference between the amount of determinant in the first cell culture and the amount of determinant in the second cell culture indicates that the composition effects the activity or expression of GSK-3β.