Patent ID: 6107055
Filing Date: 2000-08-22
Classification: C12M

Abstract:
A method for carrying out a biochemical reaction by means of a bioreactor comprising a housing and, inside said housing,(A) a reaction chamber comprising a producing system; and(B) a supply chamber containing a liquid, which liquid serves as a supply medium for said producing system, wherein said supply chamber is subdivided into first and second partial chambers, with said first and second partial chambers being(a) interconnected via a connection conduit, and(b) independently separated from said reaction chamber by a semi-permeable supply membrane,which method comprising, during the biochemical reaction, three simultaneous steps of(i) passing said liquid out of the first partial chamber into said reaction chamber;(ii) passing said liquid from said reaction chamber into the second partial chamber; and(iii) passing said liquid out of the second partial chamber via the connection conduit into the first partial chamber;in order to circulate said liquid in a flow direction of from the first partial chamber through said reaction chamber into the second partial chamber.