Patent ID: 8728810
Filing Date: 2014-05-20
Classification: A61K,A61P,C12N

Abstract:
1. A method of modifying CD44 expressed on the surface of a population of CD44-expressing cells comprising treating the population of cells in vitro with a purified α-1,3-fucosyltransferase capable of transferring 1.0 μmole of fucose to an acceptor per minute at pH 6.5 at 37° C. without the input of divalent metal co-factors and a fucose donor in a physiologically acceptable solution without the input of divalent metal co-factors, thereby forming a population of modified cells wherein the CD44 structures on the surfaces of the population of modified CD44-expressing cells bind E-selectin, and wherein the population of modified CD44-expressing cells has a viability of at least 70% at 24 hours after the treatment.