Patent ID: 7179634
Filing Date: 2007-02-20
Classification: C12N

Abstract:
1. A Bacillus cell comprising a nucleic acid construct which comprises (a) a “consensus” promoter of a Bacillus amyloliquefaciens alpha-amylase gene (amyQ) having the sequence TTGACA for the “−35” region and TATAAT for the “−10” region and having the nucleic acid sequence of SEQ ID NO:26 or SEQ ID NO:27 further operably linked to a single copy of a nucleic acid sequence encoding a polypeptide, and (b) a cryIIIA mRNA processing/stabilizing sequence located downstream of the “consensus” promoter and upstream of the nucleic acid sequence encoding the polypeptide, wherein the mRNA processing/stabilizing sequence increases expression of the nucleic acid sequence encoding the polypeptide.