Patent ID: 6107467
Filing Date: 2000-08-22
Classification: A61K,C07K

Abstract:
A process for purifying an apolipoprotein, comprisinga) mixing, in arbitrary order, a starting material containing the apolipoprotein, a first hydrophilic polymeric material, water, a second amphiphilic polymeric material and a surfactant, the first polymeric material and the second polymeric material being immiscible in the resulting primary aqueous solution, the first polymeric material comprising hydroxyalkyl starch, the second polymeric material comprising poly(oxyalkylene)copolymer, and the surfactant comprising alkyl ethoxylate;b) maintaining the primary aqueous solution for a period of time sufficient for essentially separating the solution into phases;c) removing a phase containing a main portion of the apolipoprotein and the second polymeric material;d) separating the apolipoprotein from the second polymeric material; and subsequentlye) recycling the separated second polymeric material to the initial mixing step (a).