Patent ID: 8715927
Filing Date: 2014-05-06
Classification: A61K,A61P,G01N

Abstract:
1. A method of identifying an agent that inhibits the activity of a DNA polymerase, comprising: (i) mixing a template oligodeoxynucleotide, a primer oligodeoxynucleotide and the DNA polymerase, wherein the template oligodeoxynucleotide comprises in the 5′ to 3′ direction: (ii) adding unlabeled dNTPs and a fluorescently-labeled dTTP or dATP; (iii) adding a candidate agent; and (iv) incubating the template oligodeoxynucleotide, the primer oligodeoxynucleotide, the DNA polymerase, the unlabeled dNTPs, the fluorescently-labeled dTTP or dATP, and the candidate agent for a sufficient period of time to allow DNA polymerization,