Patent ID: 7129066
Filing Date: 2006-10-31
Classification: C12N,C12Y

Abstract:
1. A fermentation process suitable for the preparation of an L-amino acid, wherein the following steps are carried out: (a) fermentation of a coryneform bacterium strain in a fermentation broth for producing the desired L-amino acid, wherein the endogenous citrate lyase E (citE) gene as set forth in SEQ ID No: 1 is inactivated by one or more methods of mutagenesis selected from the group consisting of deletion, insertional mutagenesis due to homologous recombination, and transition or transversion mutagenesis with incorporation of a non-sense mutation in the citE gene, and (b) concentration of the fermentation broth to eliminate water and increase the concentration of said L-amino acid in the broth and coryneform bacterium, and (c) isolation of the L-amino acid.