Patent ID: 7153665
Filing Date: 2006-12-26
Classification: C07K,C12N,C12Q,G01N

Abstract:
1. A method of screening for a bioactive agent that modulates IgE production, said method comprising: a) contacting, under conditions permissive for expression of an IgE fusion protein, a candidate bioactive agent and a cell, said cell comprising a genome which has been modified to express an IgE fusion protein under the control of an IL-4 inducible IgE promoter comprising the sequence set forth as SEQ ID NO:1, said IgE fusion protein comprising: i) an ε heavy chain; and ii) a fluorescent protein, and b) determining the amount of said IgE fusion protein expressed by said cell; wherein a difference in the amount of said IgE fusion protein expressed in the presence of said candidate bioactive agent as compared to the amount expressed in the absence of said candidate agent indicates that said candidate bioactive agent modulates IgE production.