Patent ID: 6872526
Filing Date: 2005-03-29
Classification: C12N,C12Q,C40B

Abstract:
1. A method for identifying bioactivities or biomolecules using high throughput screening of nucleic acid comprising: a) generating a normalized environmental gene library containing a plurality of clones in b) transferring a plurality of the clones to myceliate bacteria or myceliate fungi; c) encapsulating a bioactive substrate and at least one clone transferred in b) in a gel microdroplet, wherein a bioactivity or biomolecule produced by the clone is detectable by a change in fluorescence of the substrate prior to contacting with the at least one clone as compared to after the contacting; and d) screening the microdroplet with an assay or an analyzer that detects the presence therein of the change in fluorescence of the substrate, wherein the change indicates the identity of the bioactivity or biomolecule.