Patent ID: 8058005
Filing Date: 2011-11-15
Classification: B01L,C12Q,G01N

Abstract:
1. A qualitative method for determining one or more single nucleotide polymorphisms in one or more target nucleic acids comprising: (a) independently hybridizing over a temperature gradient one or more fluorescently tagged target amplicons to two or more target nucleic acid probes immobilized in independent areas on an upper surface of a substrate, wherein the sequences of the two or more target nucleic acid probes differ so as to represent one or more single nucleotide polymorphisms; (b) independently activating a fluorescence response from each of the one or more fluorescently tagged target amplicons hybridized to the two or more target nucleic acid probes immobilized in independent areas on the upper surface of the substrate using an evanescent wave of a predetermined wavelength; (c) independently detecting each fluorescence response; (d) independently analyzing each fluorescence response; (e) independently differentially hybridizing each of the one or more fluorescently tagged target amplicons hybridized to each of the two or more target nucleic acid probes to provide a melting curve for each of the one or more fluorescently tagged target amplicons hybridized to each of the two or more target nucleic acid probes; and (f) independently analyzing each melting curve, the T