Patent ID: 6656718
Filing Date: 2003-12-02
Classification: C07K,C12N

Abstract:
An isolated carboxypeptidase enzyme, CPG2, in which an immunogenic region is modified to reduce immunogenicity to a mammalian immune system whilst retaining CPG2 activity, wherein the immunogenic region is selected from the group consisting of: (i) KIKGRGGK (SEQ ID NO:1) comprising residues 98-105 of SEQ ID NO:7, (ii) KEYGVRD (SEQ ID NO:2) comprising residues 157-163 of SEQ ID NO:7, (iii) YGVRD (SEQ ID NO:6) comprising residues 159-163 of SEQ ID NO:7, (iv) KLADY (SEQ ID NO:3), comprising residues 191-195 of SEQ ID NO:7, (v) GAGK (SEQ ID NO:4), comprising residues 412 to the C-terminal residue 415 of SEQ ID NO:7, (vi) AG comprising residues 413-414 of SEQ ID NO:7, and (vii) EGGKKLVDK (SEQ ID NO:5) comprising residues 331-339 of SEQ ID NO:7, wherein said enzyme is fused to an anti-CEA antibody.