Patent ID: 6562565
Filing Date: 2003-05-13
Classification: C12Q,G16B

Abstract:
A method for comparing copy number of nucleic acid sequences in two or more collections of nucleic acid molecules, the method comprising:(a) providing an array comprising a plurality of target elements bound to a solid surface, each target element comprising a target nucleic acid, which target nucleic acid is not in a cell nucleus and lacks superstructure of a condensed chromosome; (b) contacting the target elements with: (i) a first collection of labeled nucleic acid molecules comprising a plurality of first nucleic acid sequences each of which specifically hybridizes to a different target nucleic acid under pre-selected hybridization conditions, and (ii) a second collection of labeled nucleic acid molecules comprising a plurality of second nucleic acid sequences each of which specifically hybridizes to a different target nucleic acid under pre-selected hybridization conditions; wherein the first and second labels are distinguishable from each other and at least one of the first and at least one of the second nucleic acid sequences hybridize to the same target nucleic acid; (c) detecting the amount of binding of the first and second labeled complementary nucleic acids to the target nucleic acids; and (d) comparing the amount of binding of the first and second labeled complementary nucleic acids to the target nucleic acids to compare copy number of nucleic acid sequences in the first and second collections that are bound to the target nucleic acids.