Patent ID: 7638608
Filing Date: 2009-12-29
Classification: A61K,C07K,C12N,G01N

Abstract:
1. A method for separating hemagglutinin (HA) HA1 and HA2 antigens from an influenza A virus or an influenza B virus, said method comprising: applying a reduced and derivatized antigen preparation comprising solubilized HA1 and HA2 antigens and a detergent in a pH controlled solution to a Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC) column, wherein a mature form of HA in an antigen preparation has been cleaved by a protease into HA1 and HA2 prior to applying the antigen preparation to the RP-HPLC column; and eluting the HA1 and HA2 antigens from the column with an ion pairing agent in an organic mobile phase, wherein the HA antigens are derivatized with an alkylating agent,