Patent ID: 6745132
Filing Date: 2004-06-01
Classification: G01N,Y10T

Abstract:
A method for determining equilibrium parameters for a macromolecule contained in a solution comprising the steps of:(a) providing a solution containing the substance, the solution being in chemical equilibrium; (b) measuring a known parameter of the solution; (c) adding a predetermined amount of a standard solution to the original solution to form a modified solution; (d) allowing the modified solution to reach chemical equilibrium; (e) remeasuring the known parameter for the modified solution; (f) repeating steps (c) through (e) for a predetermined number of iterations with the addition of the same standard solution in step (c) to the previously modified solution to obtain a plurality of measured values for the known solution parameter; (g) determining a plurality of equations that include the original solution parameter and the directly measured values of the known solution parameter; and (h) solving simultaneously the plurality of equations to obtain at least one of the desired equilibrium parameters.