Patent ID: 9193760
Filing Date: 2015-11-24
Classification: B01D,C07K

Abstract:
1. A method of purifying a target molecule from a biological sample, the method comprising in the following order, (a) contacting the sample comprising the target molecule and complexed contaminants to a metal cation-derivatized apatite solid support or a polycation-derivatized apatite solid support, wherein the metal cation is calcium, thereby non-covalently binding the target molecule to the solid support so as to provide a bound target molecule; (b) washing the bound target molecule with an agent(s) that displaces the complexed contaminants from the target molecule under conditions in which the target molecule remains substantially bound to the solid support, wherein the agent(s) comprise urea, 0.5-2.0 M sodium chloride, or both; and (c) eluting the target molecule from the solid support, wherein the eluted target molecule is substantially free of complexed contaminants.