Patent ID: 7439222
Filing Date: 2008-10-21
Classification: C07K,Y02P

Abstract:
1. A method of synthesizing a peptide, comprising steps of: a. providing a composition comprising a peptide fragment, wherein the peptide fragment comprises at least one amino acid residue and includes a base-sensitive, N-terminal protecting group; b. removing the base-sensitive, N-terminal protecting group from the peptide fragment using a deprotection reagent comprising a base, whereby an N-terminal functionality on the peptide fragment is deprotected; c. removing the base from the composition to provide a residual base content of more than 100 ppm; d. causing a reactive peptide fragment having a reactive C-terminus and a base-sensitive N-terminal protecting group to react with the deprotected N-terminal functionality of the peptide fragment under conditions such that the reactive peptide fragment is added to the peptide fragment; and e. optionally repeating steps (b) through (d) until a desired peptide is obtained.