Patent ID: 6803198
Filing Date: 2004-10-12
Classification: C07H,C07J

Abstract:
A method for detecting the presence or absence of an RNA in a biological sample suspected of containing said RNA comprising contacting said sample with a compound comprising a plurality of linked nucleosides, wherein:each nucleoside includes a ribofuranosyl sugar portion and a base portion; and at least one of said nucleosides bears at a 2â€²â€”Oâ€”position or a 3â€²â€”Oâ€”position a substituent having formula: â€”RAâ€”Nâ€”C(X)â€”Oâ€”R1a orâ€”C(X)â€”N(R1b)(R1c) â€ƒwhere: RA is alkyl having from 1 to about 10 carbon atoms; R1a is alkenyl having 2 to about 10 carbon atoms; R1b and R1c, independently, are H, R2, RA, an amine protecting group or have formula RAâ€”N(R1d)(R1e), C(X)â€”R2, C(X)â€”RA-R2, C(X)â€”Qâ€”RA-R2, or C(X)â€”Qâ€”R2; R1d and R1e, independently, are H, R2, RA, an amine protecting group or have formula C(X)â€”R2, C(X)â€”RA-R2, C(X)-Q-RA-R2, or C(X)â€”Qâ€”R2; R2 is a steroid molecule, a reporter molecule, a lipophilic molecule, a reporter enzyme, a peptide, a protein, includes folic acid, or has formula â€”Qâ€”(CH2CH2â€”Qâ€”)xâ€”R3; X is O or S; each Q is, independently, is NH, O, or S; x is 1 to about 200; R3 is H, RA, C(O)OH, C(O)ORA, C(O)R4, RAâ€”N3, or RAâ€”NH2; R4 is Cl, Br, I, SO2R5 or has structure: m is 2 to 7; and R5 alkyl having 1 to about 10 carbon atoms.