Patent ID: 8834867
Filing Date: 2014-09-16
Classification: A61K,A61P,C12N,C12Y

Abstract:
1. A preparation of yeast acid-resistant recombinant lipase LIP2, obtained by a method comprising: a) culturing b) recovering a recombinant lipase LIP2 produced by said cells from the culture supernatant, wherein the culturing is carried out in a culture medium free of products of animal origin or of uncharacterized mixtures consisting of protein materials of animal origin or of products of their enzymatic digestion, and said preparation has a catalytic activity at pH 6 of at least 15 000 units per ml of culture supernatant, one unit corresponding to the quantity of enzyme capable of catalyzing the release of 1 μmol of fatty acid per minute when the substrate used is trioctanoin, and/or in that the concentration of said lipase in said preparation is greater than 1 g of lipase per liter, wherein said