Patent ID: 9074224
Filing Date: 2015-07-07
Classification: A61K,A61P,C07K,C12N

Abstract:
1. A method of placing an exogenous nucleic acid into DNA of a cell comprising introducing into the cell: (i) an exogenous nucleic acid, (ii) a proteinaceous fusion molecule that comprises (iii) a single strand DNA probe complementary to a target site in DNA of the cell (cssDNA), with the probe and the recombinase domain complexed as a nucleoprotein filament at the time of the introduction into the cell, with the exogenous nucleic acid sequence being chosen for incorporation into the DNA of the cell and designed to be free of specific binding to the proteinaceous fusion molecule, wherein the exogenous nucleic acid is incorporated into the DNA of the cell and expressed by the cell, wherein the method is performed without introducing nucleic acids into the cell that have specific binding to the protein-protein interaction domain.