Patent ID: 8580516
Filing Date: 2013-11-12
Classification: G01N,Y10T

Abstract:
1. A method for detecting DNA damage in a subject suspected of having DNA damage characterized by the formation of aryl aldehyde moieties in DNA, comprising the steps of: a) combining a sample comprising DNA from the subject with a pro-fluorescent aromatic hydrazine compound selected from the group consisting of 2-hydrazinoquinoline, 3-methyl-2-benzothiazolinone, 6-hydrazinonicotinamide and 4-hydrazinostilbazole to label the DNA with a bis-aryl hydrazone formed between the pro-fluorescent aromatic hydrazine compound and an 5-FodU aryl aldehyde moiety formed as a result of damage to the DNA, the bis-aryl hydrazone within the DNA having a fluorescent emission distinct from the fluorescent emission of the aryl aldehyde and the aryl hydrazine; b) detecting the fluorescent DNA by monitoring the fluorescent emission of the bis-aryl hydrazone; and c) quantitating the fluorescent emission to determine the DNA damage.