Patent ID: 7638313
Filing Date: 2009-12-29
Classification: C12P

Abstract:
1. A process for producing L-threonine comprising: a) culturing or fermenting an b) isolating said L-threonine; and c) determining the amount of L-threonine isolated, wherein the expression of the yjgF open reading frame of said E. coli cell is eliminated by one or more methods of mutagenesis selected from the group consisting of: deletion of all or at least one nucleotide of the yjgF open reading frame; insertion of at least one nucleotide in the yjgF open reading frame; and transitional or transversional mutagenesis with incorporation of a non-sense mutation in the yjgF open reading frame, wherein said elimination of the expression of the yjgF open reading frame results in an increased production of L-threonine by said E. coli cell relative to the amount of L- threonine produced in said E. coli cell prior to said elimination of the expression of the yjgF open reading frame; and wherein prior to elimination of the expression of the yjgF open reading frame, said yjgF open reading frame comprises the nucleotide sequence of SEQ ID NO: 1 and encodes the polypeptide of SEQ ID NO:2.