Patent ID: 6806362
Filing Date: 2004-10-19
Classification: B01J,C12N

Abstract:
A pH dependent ion exchange matrix, comprising:a solid support, and a plurality of ion exchange ligands, each first ion exchange ligand comprising: a cap comprising an amine with a pK of less than about 9; a spacer covalently attached to the cap, the spacer comprising a spacer alkyl chain with an amine terminus and an acidic moiety covalently attached to the spacer alkyl chain, wherein the acidic moiety is a carboxyl residue; and a linker comprising a linker alkyl chain covalently attached to the solid support at a first end of the linker alkyl chain and covalently attached to the amine terminus of the spacer at a second end of the linker alkyl chain; wherein the matrix has a capacity to adsorb to a target nucleic acid at a first pH, and to release the target nucleic acid at a desorption pH which is higher than the first pH.