Patent ID: 7964380
Filing Date: 2011-06-21
Classification: B01D,B01J,B82Y,G01N

Abstract:
1. A method of manipulating biomolecules in a sample comprising the steps of: a. providing solid-phase, non-porous particles having a surface area to volume ratio greater than 10, and a density greater than 2; b. selecting a subset of the solid-phase, non-porous particles having sedimentation rates in water where the sedimentation velocity is between about 0.1 cm/min at 10,000 G (Vmin) and about 2 cm/min at 500 G (Vmax) at standard temperature and pressure, and wherein the particles form a colloidal suspension in aqueous solution; c. surface-modifying the subset of the solid-phase, non-porous particles to alter their affinity for one or more classes of biomolecules, wherein the step of surface-modifying the subset of solid-phase, non-porous particles further comprises activating the subset of solid-phase, non-porous particles, passivating the subset of solid-phase, non-porous particles, or combinations thereof; d. incubating the sample with the surface-modified particles, thereby forming a plurality of surface-modified particle-biomolecule complexes; wherein the formation of the plurality of surface-modified particle-biomolecule complexes is reversible; and e. separating the plurality of surface-modified particle-biomolecule complexes from the sample.