Patent ID: 8257920
Filing Date: 2012-09-04
Classification: C12N

Abstract:
1. An in vitro method for identifying a cell in which Notch signaling is activated, comprising the steps of: providing a gene-transferred cell comprising a first fluorescent protein (FP) gene and a second FP gene wherein the first fluorescent protein gene encodes the first fluorescent protein and is controlled by a wild-type Hes-1 gene promoter and the second fluorescent protein gene encodes the second fluorescent protein and is controlled by a mutated Hes-1 gene promoter that does not respond to an activated Notch protein, wherein the first fluorescent protein and the second fluorescent protein are different; comparing the expression of the first fluorescent protein and the second fluorescent protein in the gene-transferred cell; and identifying, as a cell in which the Notch signaling is activated, a cell in which expression of the first fluorescent protein is observed but expression of the second fluorescent protein is not observed.