Patent ID: 8280710
Filing Date: 2012-10-02
Classification: C07K

Abstract:
1. A method for determining the thermal stability of a collagen peptide, collagen-like peptide or triple-helix construct comprising a) obtaining the primary amino acid sequence of a collagen peptide, collagen-like peptide or triple-helix construct having consecutive repeating units of the tripeptide (Gly-Xaa b) identifying the number of consecutive Gly-Xaa c) determining the maximum melting temperature of the collagen peptide, collagen-like peptide or triple-helix construct relative to the melting temperature of a (Gly-Pro-Hyp) d) identifying Gly-Xaa e) determining the melting temperature of each n repeat of step d) relative to the melting temperature of a Gly-Pro-Hyp peptide; f) adjusting the melting temperature of each n repeat of step e) with a reference melting temperature for each n repeat of step e), wherein the reference melting temperature comprises: g) combining the adjusted melting temperatures of the n repeats of steps f) so that a correction melting temperature is determined for Gly-Xaa h) adjusting the maximum melting temperature of the collagen peptide, collagen-like peptide or triple-helix construct of step c) with the correction melting temperature of g) to determine the thermal stability of the collagen peptide, collagen-like peptide or triple-helix construct; and i) identifying the presence of a stabilizing sequence motif in the collagen peptide, collagen-like peptide or triple-helix construct, wherein the stabilizing sequence motif comprises Lys-Gly-Glu/Asp, and adjusting the melting temperature of step h) for the collagen peptide, collagen-like peptide or triple-helix construct when the stabilizing sequence motif is present.