Patent ID: 9169496
Filing Date: 2015-10-27
Classification: C12N,C12P

Abstract:
1. A method for the production of butadiene comprising the enzymatic conversion of crotyl alcohol into butadiene wherein the method comprises: (i) enzymatically converting crotyl alcohol into crotyl monophosphate using a hydroxyethylthiazole kinase (EC 2.7.1.50) or a thiamine kinase (EC 2.7.1.89); and (ii) enzymatically converting crotyl monophosphate into butadiene, wherein the crotyl monophosphate is directly converted into butadiene in a single enzymatic reaction using a terpene synthase selected from an isoprene synthase (EC 4.2.3.27), a myrcene/ocimene synthase (EC 4.2.3.15), a farnesene synthase (EC 4.2.3.46 or EC 4.2.3.47), a pinene synthase (EC 4.2.3.14) or a monoterpene synthase.