Patent ID: 7950183
Filing Date: 2011-05-31
Classification: A01H

Abstract:
1. A method of producing a synchronized population of conifer somatic embryo germinants in preparation for transplantation, comprising: (a) culturing conifer somatic embryos on sterile germination medium for a sufficient period of time to produce germinants comprising a visible, well-defined epicotyl stem of a desired length; (b) selecting a plurality of germinants having an epicotyl stem of the desired length; (c) storing the selected germinants having an epicotyl stem of the desired length produced in step (a) while still on the sterile germination medium from step (a) in a cold environment in which the temperature is in the range of 0.5° C. to 10° C. for a time period up to six months; and (d) transferring the germinants stored according to step (c) from the germination medium into water for a time of up to about four weeks at a temperature of about 0.5° C. to about 5° C. prior to planting the selected germinants into growth medium.