Patent ID: 6352859
Filing Date: 2002-03-05
Classification: A01H,A01K,C12N,C12Q

Abstract:
A method of producing a plant or plant cell with an improved phenotype, the method comprising:isolating genomic DNA from a source plant and fragmenting the genomic DNA; cloning the resulting fragmented genomic DNA into a cloning vector to produce a primary genomic library; transducing the primary genomic library into target plant cells; selecting the resulting transduced target plant cells, or a plant comprising transduced target plant cells for acquisition of a desired trait or property; isolating genomic DNA from a plurality of target cells which display the desired property; cloning the isolated genomic DNA from the plurality of target cells into a cloning vector to produce a secondary genomic library; transducing the secondary genomic library into the target plant cells; and, selecting the resulting transduced target plant cells, or a plant comprising transduced target plant cells for acquisition of a desired trait or property, thereby providing a plant or plant cell with an improved phenotype.