Patent ID: 9139856
Filing Date: 2015-09-22
Classification: C12P

Abstract:
1. A process for producing high purity galactooligosaccharides (GOS) by free cells, the process comprising: a) growing a mixed microbial culture of b) hydrolyzing the lactose and using the glucose produced thereby by the c) separating GOS from the mixed microbial culture using a microfiltration membrane system or centrifugation; d) filtering the GOS using a deep bed filter with cotton and activated carbon or carbon filter at a flow rate of 10-30 ml/min; e) passing permeate through a carbon polisher followed by a 0.2 micron microfiltration membrane system to remove color and suspended carbon particles; f) concentrating the GOS at a temperature range of 40-60° C. in a vacuum evaporator to obtain a syrup having 70-80% dissolved solids; g) drying the syrup to obtain high purity GOS in an amorphous form powder; and h) crystallizing the amorphous powder to obtain crystalline GOS.