Patent ID: 7115413
Filing Date: 2006-10-03
Classification: C07K,C12N

Abstract:
1. A process for making hybrid yeast cells with recombined DNA sequences comprising: (a) mutating a first set of haploid yeast cells to render defective the enzymatic mismatch repair system of the first set of cells and introducing a first DNA sequence into the first set of cells; (b) mutating a second set of haploid yeast cells to render defective the enzymatic mismatch repair system of the second set of cells and introducing a second DNA sequence into the second set of cells wherein the second DNA sequence is partially homologous to the first DNA sequence and has up to 30% base mismatches with the first DNA sequence; (c) mixing the first and second sets of cells to form diploid yeast cells; (d) culturing the diploid yeast cells to effect meiosis of the diploid yeast cells wherein said meiosis results in hybrid yeast cells comprising a recombination of the partially homologous first and second DNA sequences; and (e) recovering the hybrid yeast cells with recombined DNA sequences.