Patent ID: 6753460
Filing Date: 2004-06-22
Classification: C12N

Abstract:
A method of producing a genetically modified plant, comprising:(a) providing at least one plant cell capable of being transformed and being regenerated into a whole plant; (b) introducing into the at least one plant cell: (i) a repressible lethal gene encoding a gene product having an activity lethal to plant cells, the gene product selected from the group consisting of proteins encoded by oncogene 1 of Agrobacterium, oncogene 2 of Agrobacterium and oncogene 4 of Agrobacterium; and (ii) a sense repressor gene encoding a protein capable of repressing the activity of the gene product of the repressible lethal gene; (c) generating a plurality of whole plants from the at least one plant cell; and (d) selecting for a genetically modified plant descended from or derived from at least one of the plurality of whole plants by determining incorporation and mutually independent segregation of the repressor gene and the repressible lethal gene within the genetically modified plant.