Patent ID: 6503707
Filing Date: 2003-01-07
Classification: C07K,C12Q

Abstract:
A method for genetic typing, comprising:(a) amplifying a genetic sequence of a subject to obtain amplified DNA, which genetic sequence occurs naturally in two or more alleles each characterized by multiple polymorphisms; (b) bringing a sample of the amplified DNA into contact with an oligonucleotide probe bound to a support under stringent hybridizing conditions under which only perfect matches hybridize, which oligonucleotide probe hybridizes specifically with a DNA sequence at which a polymorphism occurs in at least one of the alleles, so that amplified DNA of one allele binds specifically with the probe and amplified DNA of at least one other amplified allele does not bind specifically with probe; (c) removing unbound amplified DNA of the sample from the support under conditions that discriminate single base-pair mismatches between the probe and the amplified DNA so that only mismatching amplified DNA is removed; (d) then denaturing and removing bound amplified DNA from the support under conditions effective to isolate a sample of such amplified DNA; and (e) then further analyzing the isolated sample to detect one of more additional polymorphisms.