Patent ID: 8471015
Filing Date: 2013-06-25
Classification: C07D,C07K

Abstract:
1. A process for producing a green fluorescent protein, which comprises reacting a compound represented by formula (4): wherein R 3 represents (i) an alkyl having 1 to 7 carbon atoms which may optionally be substituted with an alicyclic group, (ii) an alicyclic group or (iii) an arylalkyl having 7 to 10 carbon atoms, with an apoprotein of a calcium-binding photoprotein in the presence of a chelating agent, and producing the green fluorescent protein, wherein the apoprotein of a calcium-binding photoprotein is selected from the group consisting of apoaequorin, apoclytin-I, apoclytin-II, apoobelin, apomitrocomin, apomineopsin, and apobervoin, and wherein the chelating agent is selected from the group consisting of ethylenediaminetetraacetic acid, ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid, trans-1,2-diaminocyclohexane-N,N,N′,N′-tetraacetic acid, and N-(2-hydroxyethyl)iminodiacetic acid.