Patent ID: 8222031
Filing Date: 2012-07-17
Classification: A61K,A61L,C12N,G01N

Abstract:
1. An in vitro dermis equivalent, prepared in accordance with a method comprising embedding dermal fibroblasts in a three-dimensional, gel-like biomatrix containing 3.5 mg/ml up to 4.5 mg/ml of collagen in buffered serum-containing cell culture medium, whereby the biomatrix is prepared by gelling a collagen solution that has a high content of at least 90% of fresh collagen which is not denatured and not lyophilized and is prepared from rat tail tendon, and cultivating the dermal fibroblasts in such a way that an in vitro equivalent in which shrinkage in at least one of the vertical and horizontal directions has been prevented and an equivalent which has a defined diameter, uniform surface and defined termination with respect to the edge of the biomatrix is obtained.