Patent ID: 7897335
Filing Date: 2011-03-01
Classification: C12N,C12P,G01N

Abstract:
1. A gel-free method, comprising: a) providing; b) diluting at least a portion of said sample to create a plurality of diluted samples at least a portion of which contain some template and a portion contains no template; c) treating said plurality of diluted samples with said primers under conditions such that said sequences encoding said N-terminal and C-terminal markers are introduced into at least some of said nucleic acid template such that modified template encoding a protein is produced as an amplification product in ten percent of said diluted samples and no amplification product is produced in ninety percent of said diluted samples; d) introducing said modified template into said translation system under conditions such that a nascent protein is generated, said protein comprising at least said N-terminal marker; and e) testing said nascent protein under gel-free conditions that permit the detection of a truncated protein.