Patent ID: 6462809
Filing Date: 2002-10-08
Classification: B82Y,G01N,Y02A

Abstract:
A method of using critical angle refractometry for sensing presence or absence of an analyte at a binding layer, the method comprising:providing a first optically transparent element and a second optically transparent element, the first optically transparent element having a higher refractive index than that of the second optically transparent element, the second element having the binding layer; providing a contacting phase; allowing the contacting phase to contact the binding layer of the second optically transparent element; passing light through the first and the second optically transparent elements to cause the light to impinge upon an interface between the second optically transparent element and the binding layer; and detecting a location of a boundary between a light area and a dark area on a sensing element, the location of the boundary being indicative of the presence or absence of the ligands at the binding layer.