Patent ID: 7608451
Filing Date: 2009-10-27
Classification: A01K,A23L,A23V,A61K,C07K,C12N,Y02A

Abstract:
1. A vector comprising: a modified transposase gene operably linked to a first promoter, wherein the nucleotide sequence 3′ to the first promoter comprises a modified Kozak sequence as set forth in SEQ ID NO: 13, and wherein a plurality of the first twenty codons of the transposase gene are modified from the wild-type sequence by changing the nucleotide at the third base position of the codon to an adenine or thymine without modifying the amino acid encoded by the codon; and one or more genes of interest operably-linked to one or more additional promoters, wherein the one or more genes of interest and their operably-linked promoters are flanked by transposase insertion sequences recognized by a transposase encoded by the modified transposase gene wherein the vector comprises the sequence as set forth in SEQ ID NO:1.