Patent ID: 8748159
Filing Date: 2014-06-10
Classification: C12Q,Y10T

Abstract:
1. A method for measuring metabolic activity in viable yeast cells, comprising: (a) providing a sample containing yeast cells, (b) contacting said sample containing yeast cells with a molecule or dye that is altered by metabolically active viable yeast cells into a detectably fluorescent form, wherein the molecule or dye is fluorescein diacetate or OREGON GREEN™ (2′,7′ difluorofluorescein); (c) measuring fluorescence of the molecule or dye that is altered into a detectably fluorescent form by the metabolically active viable yeast cells in said sample; and (d) repeating step (c) over a time course, wherein a change in fluorescence over the time course indicates a change in metabolic activity of viable yeast cells.