Patent ID: 8128925
Filing Date: 2012-03-06
Classification: A01K,A61K,A61P,C12N,C12Q,C12Y,G01N

Abstract:
1. A purified preparation of recombinant human N-acetylgalactosamine-6-sulfatase (GALNS) enzyme, said enzyme comprising an amino acid sequence at least 95% identical to amino acids 27 to 522 of SEQ ID NO:4, useful for treating a subject suffering from a lysosomal storage disease that is caused by or associated with a deficiency in said GALNS, wherein said GALNS enzyme: (a) has a purity of at least about 95% as determined by Coomassie Blue staining when subjected to SDS-PAGE under non-reducing conditions; (b) has at least about 50% conversion of the cysteine residue at position 53 to C (c) is N-linked glycosylated at the asparagine residues at positions 178 and 397, wherein at least about 50% of the oligomannose chains attached to the asparagine residue at position 178 are bis-phosphorylated.