Patent ID: 9050565
Filing Date: 2015-06-09
Classification: B01D,B01L

Abstract:
1. A method of filtering a sample, comprising: providing a filtration device and first and second filtration device holders; said filtration device comprising a sample reservoir, a pair of spaced membranes each supported by an underdrain support comprising a series of channels, grooves or surface textures to capture filtrate, a plurality of drain holes for draining said filtrate into said vial, and a retentate chamber, said filtration device having an external diameter; each said device holder comprising a housing having an open end and a first internal diameter larger than said external diameter of said filtration device, said device holder having a shoulder within said housing spaced from said open end and defining a second internal diameter smaller than said external diameter of said filtration device; introducing sample into said sample reservoir; inserting said filtration device into said first device holder in an orientation wherein said drain holes are in fluid communication with said device holder housing; applying centrifugal force to said sample to filter said sample and cause filtrate to flow from said drain holes into said first device holder housing and to accumulate retentate in said retentate chamber; removing said filtration device from said first device holder; insert said filtration device into said second device holder in an orientation wherein said retentate chamber is in fluid communication with said second device holder housing; applying centrifugal force to said retentate in said retentate chamber to transfer said retentate into said second device holder housing; and removing said filtration device from said second device holder.