Patent ID: 8293500
Filing Date: 2012-10-23
Classification: A61K,A61P,C12Q,G01N

Abstract:
1. A method of identifying a cellular polypeptide to which a viral polypeptide binds, said method comprising: (a) contacting (i) a fraction or a supernatant obtained from a plurality of cells, and (ii) a fusion protein comprising a viral virulence polypeptide moiety fused to an affinity tag moiety, to permit the viral virulence polypeptide moiety to interact with a polypeptide associated with the cell fraction or the cell supernatant, to provide a fusion protein:cellular polypeptide complex, wherein the affinity tag moiety comprises a first polypeptide tag and a second polypeptide tag wherein the first polypeptide tag comprises a protein C-tag and the second polypeptide tag comprises a streptavidin binding peptide; (b) contacting the fusion protein:cellular polypeptide complex with a cognate ligand capable of interacting with the affinity tag, to permit formation of a cognate ligand:fusion protein:cellular polypeptide complex; (c) isolating the fusion protein:cellular polypeptide complex from the cognate ligand:fusion protein:cellular polypeptide complex by affinity purification; (d) subsequent to affinity purification, (e) identifying the cell type of a cell that comprises the cellular polypeptide to which the viral virulence polypeptide binds, said step comprising: