Patent ID: 8183354
Filing Date: 2012-05-22
Classification: A61K,A61P,C07K,C12N,G01N

Abstract:
1. A polynucleotide sequence comprising a nucleic acid sequence encoding a chimeric polypeptide comprising: a Human Immunodeficiency Virus (HIV) gp120 coat polypeptide sequence, a CD4 viral cell surface receptor polypeptide or fragment thereof, wherein the fragment is CD4D1D2, and an amino acid sequence spacer, wherein the amino acid sequence of the chimeric polypeptide is (a) a full length sequence, (b) a truncated sequence of the full length sequence, wherein the truncated sequence contains one or more deletions in constant region 1, constant region 5, variable region 1 and variable region 2 of the HIV gp120 coat polypeptide sequence, or (c) a modified sequence of the full length sequence, wherein the modified sequence has at least 95% identity to the full length sequence, and wherein the full length sequence, truncated sequence and modified sequence each have the functionality of forming a natural affinity intramolecular interacting complex between the HIV gp120 coat polypeptide and CD4 viral cell surface receptor or fragment thereof, wherein the amino acid sequence spacer is linked to both the HIV gp120 coat polypeptide sequence and the CD4 viral cell surface receptor polypeptide sequence or fragment thereof and positioned therebetween to form a single chain polypeptide of peptidic bonds and of sufficient length to allow the HIV gp120 coat polypeptide sequence and the CD4 viral cell surface receptor polypeptide sequence or fragment thereof to form the natural affinity intramolecular interacting complex by folding of the single chain polypeptide.