Patent ID: 8173393
Filing Date: 2012-05-08
Classification: A61K,A61P,C12N,C12Y,Y10S

Abstract:
1. A method of producing in a eukaryotic cell at least one fluorescent protein comprising at least one unnatural amino acid, the method comprising: growing, in an appropriate medium, a eukaryotic cell that comprises a nucleic acid that comprises at least one selector codon and encodes the fluorescent protein; wherein the eukaryotic cell comprises: an orthogonal tRNA (O-tRNA) that functions in the cell and recognizes the selector codon; and an orthogonal aminoacyl tRNA synthetase (O-RS) as set forth in any one of SEQ ID NO: 36-47 or 86, wherein the O-RS preferentially aminoacylates the O-tRNA with the unnatural amino acid, wherein the unnatural amino acid is selected from a p-acetyl-L-phenylalanine, a p-iodo-L-phenylalanine, an O-methyl-L-tyrosine, or a p-benzoyl-L-phenylalanine, and wherein said fluorescent protein is produced when said unnatural amino acid is added to said medium.