Patent ID: 7544669
Filing Date: 2009-06-09
Classification: A61K,A61P,C07K

Abstract:
1. A method of decreasing the severity of insulin dependent diabetes mellitus in a subject, the method comprising (a) administering intramuscularly a DNA plasmid vector comprising a polynucleotide encoding an autoantigen targeted in insulin dependent diabetes mellitus (IDDM), wherein the autoantigen comprises proinsulin; and (b) administering an immune modulatory sequence selected from the group consisting of 5′-Purine-Pyrimidine-[X]-[Y]-Pyrimidine-Pyrimidine-3′ and 5′-Purine-Purine-[X]-[Y]-Pyrimidine-Pyrimidine-3′ wherein X and Y are any naturally occurring or synthetic nucleotide, except that X and Y cannot be cytosine-guanine, whereby a decrease in the severity of insulin dependent diabetes mellitus in the subject is indicated by one or more measures selected from the group consisting of decreased hyperglycemia, increased plasma insulin, decreased glucosuria, decreased insulitis, decreased destruction of beta-cells, and decreased presence of autoantibodies.