Patent ID: 8163275
Filing Date: 2012-04-24
Classification: C12N

Abstract:
1. A method for obtaining a population of multi-lineage progenitor cells (MLPC) having a fibroblast morphology, said method comprising: a) contacting a human fetal blood sample with a composition, said composition comprising: b) allowing said sample to partition into an agglutinate and a supernatant phase; c) recovering cells from said supernatant phase; d) purifying MLPC from the recovered cells by adherence to a solid substrate, wherein said MLPC are positive for CD9 and positive for CD45; and e) culturing said MLPC such that said MLPC obtain said fibroblast morphology, wherein said MLPC, after obtaining said fibroblast morphology, are positive for CD9, CD13, CD29, CD44, CD73, CD90, and CD105, and negative for CD10, CD34, CD41, CD45, Stro-1, stage-specific embryonic antigen-3 (SSEA-3), and stage-specific embryonic antigen-4 (SSEA-4).