Patent ID: 8691547
Filing Date: 2014-04-08
Classification: C12N,C12Q,C12Y,G01N

Abstract:
1. A cDNA encoding a coenzyme-linked glucose dehydrogenase, comprising the sequences set forth in SEQ ID NOs. 5 to 7, the coenzyme-linked glucose dehydrogenase having the following properties (a) to (d): (a) having a subunit molecular weight of approximately 63 kDa, wherein the term “subunit molecular weight” refers to a subunit molecular weight determined by subjecting a coenzyme-linked glucose dehydrogenase without sugar chains to polyacrylamide gel electrophoresis (SDS-PAGE); (b) utilizing a FAD as a coenzyme; (c) catalyzing a reaction in which a hydroxyl group at a 1-position of glucose is oxidized and the glucose is converted to glucono-δ-lactone; and (d) having an activity toward maltose of 5% or less with respect to an activity toward glucose.