Patent ID: 6482634
Filing Date: 2002-11-19
Classification: C12N

Abstract:
A recombinant host cell containing(a) a first nucleic acid molecule comprising, from 5â€² to 3â€², a parvovirus P5 promoter, a spacer, an AAV rep gene sequence comprising a start site and encoding rep78, rep68, and other rep gene products, and an AAV a gene sequence, wherein said spacer consists of sequence heterologous to said promoter and the AAV rep gene sequence and said spacer is interposed between said promoter and the start site of the rep gene, such that said spacer is upstream of the AAV rep start site and is of sufficient size to reduce expression of the rep78 and rep68 gene products relative to other rep gene products, and (b) a second nucleic acid molecule comprising a minigene comprising a transgene flanked by AAV inverted terminal repeats (ITRs) and under the control of regulatory sequences directing expression thereof in a host cell; and (c) helper functions essential to the replication and packaging of rAAV.