Patent ID: 9075070
Filing Date: 2015-07-07
Classification: C07K,G01N

Abstract:
1. A method for identifying an agent that inhibits a Keap1-Nrf2 interaction, the method comprising: (a) providing a mixture comprising a Keap1-Kelch domain protein and a fluorescently labeled Nrf2 peptide that is capable of binding the Keap1-Kelch domain protein; (b) adding a test agent to the mixture; (c) determining the difference in the amount of fluorescently labeled Nrf2 peptide bound to the Keap1-Kelch domain protein in the presence and absence of the test agent; and (d) identifying the test agent as an inhibitor of the Keap1-Nrf2 interaction if the amount of fluorescently labeled Nrf2 peptide bound to the Keap1-Kelch domain protein in the presence of the test agent is less than in the absence of the test agent; wherein the fluorescently labeled Nrf2 peptide has the sequence Leu-Asp-Glu-Glu-Thr-Gly-Glu-Phe-Leu-NH