Patent ID: 7338765
Filing Date: 2008-03-04
Classification: C12P,C12Q

Abstract:
1. A method for non-preferential replication of a starting pool of nucleic acids, the method comprising: synthesizing cDNA from said starting pool, utilizing an oligo dT primer comprising restriction sites at least one frequent cutter restriction enzyme; synthesizing a DNA strand complementary to said cDNA to produce double stranded DNA; ligating linkers comprising restriction sites for said at least one frequent cutter restriction enzyme to the 5′ end of said double stranded DNA; digesting the product of said ligating step with said frequent cutter restriction enzyme; ligating non-phosphorylated linkers to fragments of said digesting step; amplifying the product of said ligating step with a single primer; wherein the product of said amplifying step is non-preferentially amplified about 10