Patent ID: 8932835
Filing Date: 2015-01-13
Classification: C12P

Abstract:
1. A process for the enantioselective enzymatic reduction of a keto compound of general formula I: wherein R is any protective group for amino functions and X═Cl, CN, OH, Br, or F, to the hydroxy compound of general formula II (R,S-compound): wherein R and X have the same meaning as in formula I, with the keto compound being reduced with an isolated oxidoreductase in the presence of a cofactor NADH or NADPH, wherein the oxidoreductase a) comprises an amino acid sequence according to SEQ ID No: 1, or b) is a polypeptide having greater than 75% amino acid sequence identity to SEQ ID No: 1 and enantiomeric selectivity of greater than 80% toward the hydroxyl compound of general formula II, or c) is encoded by the nucleic acid sequence SEQ ID NO:16, or d) is encoded by a nucleic acid sequence which hybridizes to a full length sequence of SEQ ID NO:16 when hybridization is carried out in a 0.7-1 M NaCl solution at 60° C. and washing is carried out with a 0.1 to 0.5-fold SSC solution at 65° C. and enantiomeric selectivity of at least 80% toward the hydroxyl compound of general formula II.