Patent ID: 8221987
Filing Date: 2012-07-17
Classification: C07K

Abstract:
1. A method of identifying a compound that putatively enhances, inhibits, or elicits bitter taste in a human subject comprising (i) screening one or more compounds in a screening assay which identifies compounds that specifically bind to or inhibit the specific binding of a ligand to a human T2R50 polypeptide, wherein said T2R50 polypeptide comprises a polypeptide sequence having the sequence in SEQ ID NO:3 or a variant thereof that possessing at least 99% sequence identity therewith and which contains at its carboxy-terminus the same 10 amino acids as the T2R50 polypeptide contained in SEQ ID NO:3 and (ii) identifying the compound as a compound that putatively enhances, inhibits, or elicits bitter taste in a human subject if the compound specifically binds to a T2R50 polypeptide possessing at least 99% sequence identity to the T2R50 polypeptide having the sequence in SEQ ID NO:3.