Patent ID: 7642059
Filing Date: 2010-01-05
Classification: G01N

Abstract:
1. A method for determining the presence or amount of an immunosuppressive drug in a sample comprising the steps of: providing a sample suspected of containing the immunosuppressive drug, mixing the sample with a conjugate and an immunophilin receptor specific for the immunosuppressive drug to form a suspension, wherein the immunophilin receptor is bound to detection particles and the conjugate comprises an exogenous immunosuppressive drug or drug analog bound to a macromolecular carrier, wherein the immunosuppressive drug present in the sample and the exogenous drug or drug analog compete for binding to the immunophilin receptor, and the detection particles agglutinate upon binding of the immunophilin receptor with the exogenous drug or drug analog, measuring the amount of particle agglutination in the suspension, and correlating the amount of agglutination measured with the presence or amount of immunosuppressive drug in the sample, wherein the immunosuppressive drug is selected from the group consisting of tacrolimus and rapamycin.