Patent ID: 7510838
Filing Date: 2009-03-31
Classification: C07K,C12Q,G01N

Abstract:
1. A method for diagnosing high-grade cervical disease in a patient independent of the patient's HPV infection status, the method comprising: a) obtaining a body sample from the patient; b) contacting the sample with at least three antibodies, wherein each of the antibodies specifically binds to a nuclear biomarker protein that is selectively overexpressed in high-grade cervical disease, wherein a first and a second antibody specifically bind to the biomarker protein MCM2, and wherein a third antibody specifically binds to the biomarker protein Topo2A; and, c) detecting binding of the antibodies to the biomarker proteins MCM2 and Topo2A to determine if the biomarker proteins are overexpressed in the sample, and thereby diagnosing high-grade cervical disease in the patient.