Patent ID: 6015881
Filing Date: 2000-01-18
Classification: C07K,C12N

Abstract:
A method for the synthesis of a peptide having the formulaAc-YTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWF-NH.sub.2 (SEQ ID NO: 1),comprising:(a) reacting a side-chain protected peptide of the formula: Fmoc-EKNEQELLEL-COOH (SEQ ID NO:11) with a side chain-protected peptide of the formula:NH.sub.2 -DKWASLWNWF-NH.sub.2 (SEQ ID NO: 18)to yield a side-chain protected peptide of the formula:Fmoc-EKNEQELLELDKWASLWNWF-NH.sub.2 (SEQ ID NO: 12);(b) deprotecting the amino terminus of the peptide produced in (a);(c) reacting the peptide produced in (b) with a side-chain protected peptide of the formula:Fmoc-YTSLIHSLIEESQNQQ-COOH (SEQ ID NO:4)to yield a side-chain protected peptide of the formula:Fmoc-YTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWF-NH.sub.2 (SEQ ID NO: 1);(d) modifying the amino terminus of the peptide produced in (c) into an acetyl modification; and(e) deprotecting the side chains of the side-chain protected peptide of (d) to yield a peptide of the formula:Ac-YTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWF-NH.sub.2 (SEQ ID NO: 1).2.