Patent ID: 8530195
Filing Date: 2013-09-10
Classification: C07D,C07H,C09B,C12Q

Abstract:
1. A method of performing a nucleic acid amplification reaction comprising: (i) providing a reaction mixture comprising said sample and reagents necessary for amplifying nucleic acid; (ii) subjecting said reaction mixture to a polymerization reaction under conditions suitable for formation of amplified nucleic acid; (iii) adding a fluorescent nucleic acid dye, wherein said addition takes place before, during, or after step (ii), and wherein said dye has the formula: wherein at least one of Q 1 and Q 2 is a fluorescent nucleic acid dye constituent; and R r is a reactive group for covalent attachment to a nucleic acid; (iv) illuminating the reaction mixture with light; and (v) detecting fluorescence emission from said reaction mixture, wherein said emission is indicative of the presence of nucleic acid, wherein B is a nucleotide or an oligonucleotide; BRIDGE is a substantially aliphatic linker comprising from about 15 to about 150 non-hydrogen atoms and up to one positive charge; and each of Q wherein each of L 1 and L 2 , independently, is a moiety comprising a single bond; a polymethylene unit having 1 carbon to about 12 carbons, inclusive, optionally comprising at least one hetero atom selected from N, O and S; or an aryl optionally comprising at least one hetero atom selected from N, O and S; each of A each of α, β, γ, δ, ε, ζ, η, θ, and τ, independently, is zero or an integer from 1 to about 20, inclusive; each of a, b, c, d, e, f, g, h, and i, independently, is zero or an integer from 1 to about 20, inclusive; and one of A B′ has the formula: each of a′, b′, and c′ is independently zero or an integer from 1 to about 20, inclusive.