Patent ID: 6905885
Filing Date: 2005-06-14
Classification: G01N

Abstract:
1. A method for detection and measurement of a targeted biological sample, comprising the steps of: providing a multiplicity of optically encoded microbeads, providing said optically encoded microbeads with a capture ligand, providing said optically encoded microbeads with bioagent-specific antibodies, placing said optically encoded microbeads into a container, adding a sample to said contained optically encoded microbeads, said sample possibly containing the targeted biological sample, placing said contained optically encoded microbeads and said sample in a mixing holder for sufficient time for the targeted biological sample to adequately bind said optically encoded microbeads, adding fluorescent labeled antibodies to said contained optically encoded microbeads and said sample for attachment to said bioagent-specific antibodies, attaching at least some of said optically encoded microbeads to a disposable capture substrate containing an array of individual attachment sites for attaching said optically encoded microbeads thereto wherein each individual attachment site captures a single optically encoded micro bead with capture ligand, bioagent-specific antibody, fluorescent labeled antibody, and any targeted biological sample. washing said substrate and attached optically encoded microbeads, inserting said substrate into an optical detection system, and optically decoding said optically encoded microbeads by identifying said optically encoded microbeads and said fluorescent labeled antibodies for detection and measurement of the targeted biological sample.