Patent ID: 6653473
Filing Date: 2003-11-25
Classification: C07B,C07C,C07D,C07F,C07H,C09B,C12Q,C40B

Abstract:
A resorufin phosphoramidite reagent having the formula: wherein,Z is O or S; each of R21, R22, R23, R24, R25, R26 and R27 is independently selected from H, halogen, NO2, SO3R, SO2N(R)2, C(O)OR, C(O)N(R)2, CN, CNS, OR, OC(O)R, SR, CF3, NHC(O)R, N(R)2, N[R]3 and â€”Lâ€”X; wherein each R is independently H, (C1-C8)alkyl, aryl or heteroaryl, or a blocking group compatible with oligonucleotide synthesis; â€”Lâ€”X is a linker of from 1 to 20 atoms, that is acyclic, cyclic or a combination thereof, having main chain atoms selected from C, N, O, S, Si and P; and X is a phosphoramidite; with the proviso that at least one of R21, R22, R23, R24, R25, R26, and R27 is â€”Lâ€”X.