Patent ID: 8206935
Filing Date: 2012-06-26
Classification: C12Q

Abstract:
1. A method of rapidly quantifying hydroxymethylated DNA in a DNA sample comprising: a) the binding of the DNA sample to a plastic carrier at an appropriate temperature for an appropriate time period with a DNA binding solution containing at least three salt reagent selected from sodium and ammonium salts or contains multiple sodium and ammonium salts at an appropriate concentration and in an appropriate ratio of each salt; wherein the appropriate temperature is from 25° C. to 37° C., the sodium salt is sodium chloride in an amount of 0.3 M to 3M, or sodium sulfate in an amount of 0.5 M to 3M, and the ammonium salt is ammonium chloride in an amount of 0.06 M to 3M or ammonium sulfate in an amount of 0.3 M to 3M, wherein the appropriate ratio is sodium chloride to ammonium sulfate to ammonium chloride at 5:5:1 in said binding solution; b) the addition of an anti-5-hydroxymethylcytosine structure antibody that reacts with 5-hydroxymethylcytosine contained in the DNA to form immuno-reaction products; c) the determination of said immuno-reaction products with a signal reporter; and d) the quantification of hydroxymethylcytosine amount in said DNA sample by comparing signal intensity generated from said DNA sample with that from hydroxymethylated DNA standard control.