Patent ID: 9140707
Filing Date: 2015-09-22
Classification: B01L,C07K,G01N

Abstract:
1. A method of diagnosing Factor V deficiency by determining a presence and quantity of Factor V Leiden (FVL) and Normal Factor V (FVN) in a sample from a subject comprising: providing a first monoclonal antibody and a second monoclonal antibody, wherein the first monoclonal antibody was obtained against a peptide comprising FVN-20mer (SEQ ID NO 1): H-I-C-K-S-R-S-L-D-R-R-G-I-Q-R-A-A-D-I-E-Q-NH wherein the second monoclonal antibody was obtained against a peptide comprising FVL-20-mer (SEQ ID NO: 2): H-I-C-K-S-R-S-L-D-R-Q-G-I-Q-R-A-A-D-I-E-Q-NH immobilizing the first antibody and the second antibody on first and second optical sensing fibers, respectively; exposing the sample to each of the immobilized antibodies separately to bind FVN and/or FVL in the sample to the optical sensing fibers; probing the first and second sample-exposed optical sensing fibers with a fluorophore-tagged antibody that binds to both bound FVN and FVL with equal affinity; obtaining fluorescent signal intensity values from bound fluorophore-tagged antibody for each of the first and second optical sensing fibers; and determining respective concentrations of FVN and FVL in the sample from said obtained signal intensity values, and determining if Factor V deficiency exists in the subject and/or severity of the deficiency in the subject from the determined concentrations of FVN and FVL in the sample.