Patent ID: 8105575
Filing Date: 2012-01-31
Classification: C12N

Abstract:
1. A retroviral vector comprising an RPL10 promoter consisting of the nucleotide sequence of SEQ ID NO: 8 or a fragment thereof as an internal promoter; and a 5′ long terminal repeat (LTR) and a 3′ LTR, wherein the 3′ LTR lacks an enhancer element of the U3 region thereof, and wherein the fragment comprises: (i) the sequence from about −350 to about −1 relative to the transcription start site of the RPL10 promoter, said sequence from about −350 to about −1 being the nucleotides at positions from about 651 to about 1000 of SEQ ID NO: 8; (ii) the sequence from about −50 to about +143 relative to the transcription start site of the RPL10 promoter, said sequence from about −50 to about +143 being the nucleotides at positions from about 951 to about 1143 of SEQ ID NO: 8; (iii) the sequence from about −100 to about +143 relative to the transcription start site of the RPL10 promoter, said sequence from about −100 to about +143 being the nucleotides at positions from about 901 to about 1143 of SEQ ID NO: 8; (iv) the sequence from about −200 to about +143 relative to the transcription start site of the RPL10 promoter, said sequence from about −200 to about +143 being the nucleotides at positions from about 801 to about 1143 of SEQ ID NO: 8; (v) the sequence from about −350 to about +143 relative to the transcription start site of the RPL10 promoter, said sequence from about −350 to about +143 being the nucleotides at positions from about 651 to about 1143 of SEQ ID NO: 8; or (vi) the sequence from about −500 to about +143 relative to the transcription start site of the RPL10 promoter, said sequence from about −500 to about +143 being the nucleotides at positions from about 501 to about 1143 of SEQ ID NO: 8.