Patent ID: 7749754
Filing Date: 2010-07-06
Classification: A61K,C12N

Abstract:
1. A method of enrichment for a composition comprising a population of human or mouse myogenic progenitor cells, wherein at least 80% of the cells in said population are myofiber associated, CD45-, Sca-1-, Mac-1-, CXCR4+, and β1-integrin+; the method comprising: dissociating human or mouse muscle tissue to provide a population of myofiber associated cells; combining reagents that specifically recognize Mac-1, CXCR4, β-1integrin, and CD45 respectively, with said population of myofiber associated cells; selecting for those cells that are CD45-, Sca-1-, Mac-1-, CXCR4+, and β1-integrin +; wherein said selected population of cells are capable of forming myogenic colonies.