Patent ID: 8563286
Filing Date: 2013-10-22
Classification: C12N

Abstract:
1. A nucleic acid construct comprising an isolated polynucleotide encoding a polypeptide having lipase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct the production of the polynucleotide in an expression host and wherein the polynucleotide is selected from the group consisting of: (a) a polynucleotide encoding a polypeptide having lipase activity comprising an amino acid sequence having at least 90% identity with the mature polypeptide of SEQ ID NO: 6; (b) a polynucleotide encoding a polypeptide having lipase activity comprising a nucleotide sequence which hybridizes under at least high stringency conditions with the mature polypeptide coding sequence of SEQ ID NO: 5 or its full-length complementary strand, wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 μg/ml sheared and denatured salmon sperm DNA, and 50% formamide and a wash three times, each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.; and (c) a polynucleotide encoding a polypeptide having lipase activity comprising a nucleotide sequence having at least 90% identity with the mature polypeptide coding sequence of SEQ ID NO: 5.