Patent ID: 6844148
Filing Date: 2005-01-18
Classification: A61P,C07K,C12N,C12Q,G01N

Abstract:
1. A method for assaying hu-Asp1 APP proteolytic activity in a cell free or cell culture system comprising the steps of: (a) contacting hu-Asp1 enzyme with an amyloid precursor protein (APP) substrate, wherein the hu-Asp1 enzyme is a recombinant polypeptide expressed by a host cell transformed or transfected with a nucleic acid molecule that comprises a nucleotide sequence that encodes an amino acid sequence at least 95% identical to SEQ ID NO: 2 or to a fragment of SEQ ID NO: 2, wherein the polypeptide retains APP proteolytic activity, and wherein said substrate contains a hu-Asp1 APP cleavage site; and (b) measuring cleavage of the APP substrate at the hu-Asp1 APP cleavage site, thereby assaying hu-Asp1 APP proteolytic activity.