Patent ID: 7537903
Filing Date: 2009-05-26
Classification: G01N

Abstract:
1. A method to identify a modulator of βKlotho-dependent glucose transporter-1 (GLUT-1) upregulation that specifically modulates interaction of βKlotho and an FGFR, the method comprising steps: contacting an agent with a βKlotho-dependent GLUT-1 assay system under conditions wherein but for the presence of the agent, the assay has a reference GLUT-1 readout; detecting an agent-biased specific modulation of the GLUT-1 readout significantly different from the reference readout, indicating that the agent is a modulator of GLUT-1 upregulation; and determining that the agent specifically modulates the interaction of the βKlotho and the FGFR, indicating that the modulation of the GLUT-1 upregulation is βKlotho-dependent, wherein the assay system comprises cultured 3T3-L1 adipocytes, the GLUT-1 readout is glucose uptake in the adipocytes, the FGFR is FGFR1c or FGFR4, the determining step is performed by a βKlotho-FGFR binding assay, and the agent is a compound of a drug screening compound library, a putative FGF21protein mimetic, or an antibody.