Patent ID: 6284509
Filing Date: 2001-09-04
Classification: A61K,C07K,C12N,C12Y,Y02A

Abstract:
An isolated enzyme exhibiting beta-1,3-glucanase activity and having a mannose binding domain, wherein the enzyme is selected from the group consisting of:(a) the enzyme encoded by the mature protein-encoding portion of the DNA sequence of SEQ ID NO:10;(b) a polypeptide encoded by a DNA sequence which hybridizes to SEQ ID NO:10 under the following conditions: pre-hybridized in 50% formamide, 6.times.SSC, 0.05.times.BLOTTO, 1 mM EDTA at 42.degree. C. for 1-2 hours, followed by hybridization with the radiolabelled and denaturated DNA probe in 50% formamide, 6.times.SSC, 0.5% (w/v) SDS, 1 mM EDTA at 42.degree. C., overnight, followed by one wash in 2.times.SSC, 0.5% SDS, and a second wash with 1.times.SSC, 0.5% SDS at 50.degree. C.;(c) a polypeptide having the amino acid sequence of SEQ ID NO:11, and(d) a polypeptide which is 90% homologous with the polypeptide encoded by the DNA sequence of (a).