Patent ID: 6365407
Filing Date: 2002-04-02
Classification: A01H,C12N,C12P

Abstract:
A culture medium for induction and proliferation of callus lines from explants, the culture medium comprisingA) Macronutrientsin the amounts of:KNO3about 2528 mg Â· Lâˆ’1MgSO4.7H2Oabout 246 mg Â· Lâˆ’1(NH4)2SO4about 134 mg Â· Lâˆ’1NaH2PO4about 150 mg Â· Lâˆ’1CaCl2.2H2Oabout 150 mg Â· Lâˆ’1Na2-EDTAabout 37.2 mg Â· Lâˆ’1FeSO4.7H2Oabout 27.8 mg Â· Lâˆ’1;B) Micronutrients inthe amount of:H3BO3about 3.0 mg Â· Lâˆ’1CoCl2.6H2Oabout 0.025 mg Â· Lâˆ’1CuSO4.5H2Oabout 0.025 mg Â· Lâˆ’1MnSO4.H2Oabout 10.0 mg Â· Lâˆ’1ZnSO4.7H2Oabout 2.0 mg Â· Lâˆ’1KIabout 0.75 mg Â· Lâˆ’1Na2MoO4,.2H2Oabout 0.25 mg Â· Lâˆ’1;C) Vitamins in the amounts of:Myo-inositolabout 100 mg Â· Lâˆ’1Nicotinic acidabout 1.0 mg Â· Lâˆ’1Pyridoxin.HClabout 1.0 mg Â· Lâˆ’1Thiamine.HClabout 10.0 mg Â· Lâˆ’1; and D) Other Ingredientsin the amounts of:Picloramabout 0.1-about 20.0 mg Â· Lâˆ’1Sucroseabout 10,000-about 40,000 mg Â· Lâˆ’1Phytagelabout 2.0-about 4.0 mg Â· Lâˆ’1Antioxidantsabout 1.0-about 5.0 mg Â· Lâˆ’1.