Patent ID: 6040182
Filing Date: 2000-03-21
Classification: G01N

Abstract:
A method for achieving efficient and stable protein coating on plasma treated plastic surfaces wherein said method comprises:(a) coating a plasma treated plastic surface with a coating solution wherein said coating solution comprises a protein or polypeptide to be immobilized in a concentration of about 1 ng/mL to about 2000 ng/mL, in a coating buffer, wherein said coating buffer has a buffer species dissolved in distilled or deionized water in a concentration of about 2 mM to about 500 mM and the pH of the coating buffer is from about 6 to about 8;(b) incubating said coating solution on said plasma treated plastic surface;(c) washing the plastic surface to remove any extraneous materials not firmly attached to the plastic surface;(d) drying the plastic surface having the coating solution thereon; and(e) obtaining a plasma treated plastic surface having a protein efficiently and stably bound thereto.