Patent ID: 8309099
Filing Date: 2012-11-13
Classification: A61K,A61P,C07K,C12N

Abstract:
1. A method of preparing a vaccine comprising a segmented negative strand RNA virus virion, which method comprises 1) culturing a host cell comprising a minimum plasmid-based system, wherein the minimum plasmid-based system comprises a set of plasmids, each plasmid of said set comprises one viral genomic segment of said negative strand RNA virus, and wherein the viral cDNA corresponding to the viral genomic segment is inserted between an RNA polymerase I (pol I) promoter and a regulatory element, which results in expression of vRNA or cRNA with an exact 3′ end, which are in turn inserted between an RNA polymerase II (pol II) promoter and a polyadenylation signal, which results in expression of viral mRNA and a corresponding viral protein, wherein the expression of vRNAs or cRNAs and viral proteins from said set of plasmids results in a segmented negative strand RNA virus virion, wherein the total number of the plasmids in the minimum plasmid-based system does not exceed the total number of the gene segments of the negative strand RNA virus, 2) purifying the negative strand RNA virus virion produced in the host cell, and 3) producing a vaccine comprising the purified virion.