Patent ID: 8632986
Filing Date: 2014-01-21
Classification: G01N

Abstract:
1. A method for detecting toxemia in a subject patient, comprising the steps of: mixing a labeled hydrophobic probe capable of binding to carrier proteins with an aliquot of a subject patient's extracellular fluid containing carrier proteins, the amount of probe such that the molar ratio of the probe to the carrier proteins is between about 0.3 and about 1.5; mixing a solvent with the aliquot, the solvent such that when added to the aliquot and probe the solubility of the probe is increased in the aliquot, and the amount of solvent sufficient to dissociate a portion of the probe from the carrier proteins without causing significant dissociation of toxins from the carrier proteins; analyzing the mixture comprising the aliquot, probe, and solvent to determine the binding efficiency of the carrier proteins; and comparing the subject binding efficiency to at least one control binding efficiency for at least one non-toxemic control patient.