Patent ID: 6329151
Filing Date: 2001-12-11
Classification: C07K,C12Q

Abstract:
A method for the identification of differentially expressed genes comprising:(i) separating a first and second population of microbial cells, wherein the first population of cells is contacted with a stimulating agent;(ii) extracting total RNA from the first and second population of microbial cells of step (i);(iii) amplifying the extracted RNA of the first and second populations of microbial cells by a process comprising:a) preparing a collection of at least 32 different arbitrary primers, each primer comprising a common region and a variable region;b) individually contacting each different primer of step (a) with a sample of the extracted RNA from the first and second population of microbial cells under conditions wherein a set of first and second amplification products are produced;(iv) purifying the first and second amplification products of step (iii);(v) identifying the amplification products generated from the first population of microbial cells that differ from the amplification products generated from the second population of microbial cells as differentially expressed genes; and(vi) optionally sequencing the identified differentially expressed genes of step (v).