Patent ID: 8293465
Filing Date: 2012-10-23
Classification: A61P,C07K

Abstract:
1. A method of detecting human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein comprising the following: (a) preparing a solid surface to which is bound a known quantity of carbohydrate binding module (CBM) 20 or 21; (b) contacting the solid surface with a subject sample; (c) incubating the sample with the surface under conditions that facilitate CBM binding to any HIV-1 envelope glycoprotein present in the sample thereby forming a CBM-envelope complex; (d) continuing the incubation under conditions that facilitate binding of the CBM-envelope complex to anti-HIV-1 antibodies that may be present in the sample thereby forming a CBM-envelope-anti-HIV-1 antibody complex; (e) adding a labeled HIV-1 envelope conjugate to the mixture, wherein the conjugate binds to the anti-HIV-1 antibody present in the CBM-envelope-anti-HIV-1 antibody complex, and (f) measuring the signal intensity from the labeled complex.