Patent ID: 6165785
Filing Date: 2000-12-26
Classification: A61K,C12N

Abstract:
A method of treatment comprising the steps of:(a) obtaining stem cells from bone marrow;(b) combining the stem cells with splenocytes to form a co-culture;(c) adding a first factor selected from the group consisting of lipopolysaccharide, interleukin-6, interleukin-4, tumor necrosis factor alpha, transforming growth factor beta, anti-CD3 and mixtures thereof to the co-culture;(d) adding a second factor selected from the group consisting of granulocyte macrophage colony-stimulating factor, macrophage-colony stimulating factor, granulocyte-colony stimulating factor, FLT3 ligand and mixtures thereof to the co-culture;(e) maintaining the co-culture for at least 4 days;(f) obtaining differentiated immune system suppressor cells and immune system stimulator cells from the co-culture; and(g) introducing the immune system suppressor cells and immune system stimulatory cells into a host;wherein the immune system suppressor cells are capable of prolonging allograft survival when infused as a donor specific transfusion to a transplant patient.