Patent ID: 7718048
Filing Date: 2010-05-18
Classification: C12Q

Abstract:
1. A base sequence detecting method comprising: detecting electrochemical signals at a detecting electrode and a comparison electrode of a base sequence detecting device comprising: the conductive detecting electrode; first blocking molecules formed so as to cover a surface of the detecting electrode, the first blocking molecules decreasing adsorption of an intercalating agent to the surface of the detecting electrode; a target-complementary probe immobilized to the detecting electrode via a first spacer member comprising straight chain organic molecules, the target-complementary probe including a base sequence complementary to a target base sequence which is an object of detection; the conductive comparison electrode; and second blocking molecules formed so as to cover a surface of the comparison electrode, the second blocking molecules decreasing adsorption of an intercalating agent to the surface of the comparison electrode; and subtracting an electrochemical signal detected at the comparison electrode from an electrochemical signal detected at the detecting electrode, wherein the base sequence detecting device further comprising a dummy probe immobilized to the comparison electrode via a second spacer member comprising straight chain organic molecules, the dummy probe including a base sequence noncomplementary to the target base sequence which is the object of detection, and wherein the detecting electrode is configured to detect a probe current caused by interaction between the intercalating agent and the target-complementary probe hybridized with the target, the comparison electrode is configured to detect background current.