Patent ID: 8460920
Filing Date: 2013-06-11
Classification: C12N

Abstract:
1. A method for purifying adenovirus particles from a suspension of producer cells having a cell density between about 10×10 6 and 50×10 6 cells/mL, the method comprising the steps of: (a) infecting a producer cell suspension having a cell density between about 10×10 (b) lysing with detergent the producer cells within the cell suspension having a cell density between about 10×10 (c) selectively precipitating producer cell DNA away from the adenovirus particles by adding domiphen bromide (DB) to the suspension to a final concentration within the range of 1.3 to 2.2 mM; and then (d) clarifying the suspension comprising adenovirus particles to obtain a purified adenovirus-containing suspension, wherein at least 80% of producer cell DNA is precipitated away from the suspension comprising adenovirus particles, wherein steps (b), (c) and (d) are performed consecutively.