Patent ID: 8809055
Filing Date: 2014-08-19
Classification: A61K,A61P,C07K,C12N,C12Y

Abstract:
1. A method of concentrating a solution, the method comprising: (a) providing a solution that comprises α-mannosidase or an analogue thereof, wherein the analogue has (i) at least 95% sequence identity to the α-mannosidase of SEQ ID NO: 21 and (ii) at least 50% of the activity of the α-mannosidase; (b) removing α-mannosidase aggregates from the solution by centrifugation or filtration; and then (c) concentrating the solution, wherein the concentrated solution (i) is isotonic to mammalian tissue, (ii) comprises at least 25 mg/ml of the α-mannosidase or analogue, and (iii) comprises less than 5% w/w aggregated α-mannosidase or analogue based on the total α-mannosidase or analogue in the concentrated solution.