Patent ID: 6127140
Filing Date: 2000-10-03
Classification: C12Q,Y10S

Abstract:
A method for determining concentration of an analyte in a biological sample comprising the steps of:(a) combining the biological sample, at least one oxidizing enzyme for the analyte of interest, nicotinamide adenine dinucleotide, and a chemiluminescent label to form a reaction mixture;(b) allowing the analyte to undergo an oxidation-reduction reaction and nicotinamide adenine dinucleotide to be converted to the reduced form of nicotinamide adenine dinucleotide and further allowing the chemiluminescent label to react with the reduced form of nicotinamide adenine dinucleotide; and(c) determining the concentration of the analyte of interest in the biological sample by correlating the quantity of light emitted with the concentration of the reduced form of nicotinamide adenine dinucleotide.