Patent ID: 9012170
Filing Date: 2015-04-21
Classification: C12N,C12Q,G01N

Abstract:
1. An imaging method, comprising: providing a sample containing a cell that expresses a split peroxidase comprising two or more separate components of a peroxidase, and contacting the sample with a peroxidase substrate to allow conversion of the peroxidase substrate into a product via an enzymatic reaction catalyzed by a reconstituted peroxidase that forms when the two or more components of the split peroxidase interact, wherein the product releases a detectable signal, wherein said split peroxidases are capable of performing any of the following: converting said product without any assistance from additional protein-protein interaction, enduring chemical fixation, ultra-sensitive synapse detection, achieving greater sensitivity than split-GFP constructs or converting said product with assistance from a protein-protein interaction.