Patent ID: 6362319
Filing Date: 2002-03-26
Classification: A61K,A61P,C07K,Y10S

Abstract:
A purified and isolated glial cell line-derived neurotrophic factor (GDNF) polypeptide encoded by a polynucleotide, wherein said polynucleotide:(a) comprises nucleotides 304 through 705 of SEQ ID NO:3 or nucleotides 105 through 506 of SEQ ID NO:5; or (b) encodes a polypeptide comprising an amino sequence set forth in SEQ ID NO:4 or SEQ ID NO:6; or (c) hybridizes to an oligonucleotide probe fully complementary to a polynucleotide encoding the amino acid sequence of SEQ ID NO:4 under conditions comprising hybridizing in 6X SSPE, 0.1% SDS and 30% formamide at 42Â° C., followed by washing in 2X SSPE and 0.1% SDS at room temperature and twice in 0.1 x SSPE, 0.1% SDS preheated to 50Â° C. and wherein said polypeptide promotes dopamine uptake in dopaminergic neorons; or (d) hybridizes to an olignucleotide probe fully complementary to a polynucleotide encoding the amino acid sequence of SEQ. ID. NO:6 under conditions comprising hybridizing in 6X SSPE and 0.1% SDS at 50Â° C., followed by washing in 2X SSPE and 0.1% SDS at room temperature and twice in 0.1 x SSPE, 0.1% SDS preheated to 50Â° C. and wherein said polypeptide promotes dopamine uptake in dopaminergic neurons.