Patent ID: 6054321
Filing Date: 2000-04-25
Classification: C07K,Y10S,Y10T

Abstract:
A method of engineering a functional mutant of a second fluorescent protein comprising the steps of,1) displaying the atomic coordinates of FIGS. 5-1 to 5-28 to form a three-dimensional structure of a first fluorescent protein,2) constructing a model of a second fluorescent protein using the structure of said first fluorescent protein as a template,3) identifying at least one amino acid residue within about 5 .ANG. from any atom of the chromophore of said second fluorescent protein in the modeled structure,4) making a mutant of the second fluorescent protein in which at least one of the identified amino acid residue in step 3 is substituted with a different amino acid,wherein said second fluorescent protein has an amino acid sequence at least 80% identical to SID. NO:2,wherein said mutant second fluorescent protein has a different fluorescent property than said second fluorescent protein,wherein said chromophore is generated by the cyclization and oxidation of amino acids of said second fluorescent protein, andwherein said chromophore comprises a p-hydroxycinnamyl group.