Patent ID: 7402427
Filing Date: 2008-07-22
Classification: A61K,A61P,C07K,C12N

Abstract:
1. A complex comprising a genomic RNA of paramyxovirus wherein the genomic RNA comprises: (a) a nucleic acid encoding an M protein that is mutated or deleted, (b) a nucleic acid encoding a modified F protein, whose cleavage site sequence is substituted with a sequence that can be cleaved by a protease that does not cleave the wild-type F protein, and wherein the complex comprises the wild-type F protein, said complex further comprising the following properties: (1) the ability to replicate the genomic RNA in a cell to which the complex has been introduced; (2) a significant decrease in or lack of production of viral particles in an intrahost environment; and (3) the ability to introduce the RNA into a cell that contacts with a cell infected with the complex in the presence of the protease.