Patent ID: 7553613
Filing Date: 2009-06-30
Classification: A61K,A61P,C12N,Y10S

Abstract:
1. A method for preparing a hemoglobin-based oxygen carrier, comprising the steps of: a) defibrinating whole blood, wherein the whole blood comprises red blood cells and a plasma component; b) filtering the defibrinated whole blood by diafiltration across a membrane having a permeability limit in a range of between about 0.01 μm and about 5 μm whereby at least a portion of the plasma component is separated from the red blood cells to form a red blood cell suspension; c) releasing hemoglobin molecules from the red blood cells of the red blood cell suspension by lysing the red blood cells of the red blood cell suspension and isolating the hemoglobin molecules by centrifuging or filtering the lysed red blood cell suspension; and d) stabilizing the hemoglobin, thereby preparing the hemoglobin-based oxygen carrier.