Patent ID: 6084076
Filing Date: 2000-07-04
Classification: C07K

Abstract:
A method of refolding denatured human activin A into natural-type human activin A having a biological activity, comprising:(a) protecting thiol groups in solubilized denatured human activin A with all thiol groups free, with glutathione and/or sodium sulfite, and (b1) dialyzing the protected denatured human activin A against a refolding buffer and then adding a thiol compound thereto, to exchange disulfide bonds in the denatured activin A and produce the human activin A having a biological activity, whereinthe refolding buffer contains containing taurodeoxycholic acid or a salt thereof, andthe refolding buffer permits the structure of natural-type human activin A dissolved at a concentration of 0.1 mg/ml in the refolding buffer, when denatured by reduction with 0.2 mM dithiothreitol (DTT), to be maintained stably by 20% or more than by a Tris buffer containing 1.5M urea, 20 mM Tris-HCl, at pH 8.5.