Patent ID: 6322987
Filing Date: 2001-11-27
Classification: C07H,C07J

Abstract:
A method for modulating the production of a protein by an organism comprising contacting an organism with a compound comprising a plurality of linked nucleosides, wherein:each nucleoside includes a ribofuranosyl sugar portion and a base portion, andat least one of said nucleosides bears at a 2'-O-position or a 3'-O-position a substituent having formula:--R.sub.A --N--C(X)--O--R.sub.1aor--C(X)--N(R.sub.1b)(R.sub.1c)where:R.sub.A is alkyl having from 1 to about 10 carbon atoms or (CH.sub.2 --CH.sub.2 --Q).sub.x ;R.sub.1a is alkenyl having 2 to about 10 carbon atoms;R.sub.1b and R.sub.1c, independently, are H, R.sub.2, R.sub.A, an amine protecting group or have formula R.sub.A --N(R.sub.1d)(R.sub.1e), C(X)--R.sub.2, C(X)--R.sub.A --R.sub.2, C(X)--Q--R.sub.A R.sub.2, or C(X)--Q--R.sub.2 ;R.sub.1d and R.sub.1e, independently, are H, R.sub.2, R.sub.A, an amine protecting group or have formula C(X)--R.sub.2, C(X)--R.sub.A --R.sub.2, C(X)--Q-R.sub.A --R.sub.2, or C(X)--Q--R.sub.2 ;R.sub.2 is asteroid molecule, a reporter molecule, alipophilic molecule, a reporter enzyme, a peptide, a protein, includes folic acid, or has formula --Q--(CH.sub.2 CH.sub.2 --Q--).sub.x --R.sub.3 ;X is O or S;each Q is, independently, is NH, O, or S;x is 1 to about 200;R.sub.3 is H, R.sub.A, C(O)OH, C(O)OR.sub.A, C(O)R.sub.4, R.sub.A --N.sub.3, or R.sub.A --NH.sub.2 ;R.sub.4 is Cl, Br, I, SO.sub.2 R.sub.5 or has structure: ##STR2##m is 2 to 7; andR.sub.5 alkyl having 1 to about 10 carbon atoms.