Patent ID: 8119382
Filing Date: 2012-02-21
Classification: C12N

Abstract:
1. A variant KpnI restriction endonuclease having at least 90% sequence identity with SEQ ID NO: 13 wherein the amino acid at the position corresponding to amino acid 163 of SEQ ID NO: 13 is not Asp (D); the amino acid at the position corresponding to amino acid 165 of SEQ ID NO: 13 is not Lys (K); the amino acid at the position corresponding to amino acid 148 of SEQ ID NO: 13 is not a Asp (D); the amino acid at the position corresponding to amino acid 175 of SEQ ID NO: 13 is not Gln (Q); or the amino acids at the positions corresponding to amino acids 163 and 165 of SEQ ID NO: 13 are not Asp (D) and Lys (K), respectively.