Patent ID: 7825270
Filing Date: 2010-11-02
Classification: C07F

Abstract:
1. A method of isolating and purifying inositolphospholipids (IPL) comprising phosphatidylinositol (PI) from starting materials comprising natural plant source lipids or fractions thereof, wherein said natural plant source lipids or fractions contain IPL, comprising the steps of: a. treating said starting materials with an anhydride and separating an alcohol-insoluble precipitate material from an alcohol-soluble extract solution; b. repeating said extraction and separation step (a) until said precipitate is substantially free of components that are less polar than PI, wherein said precipitate is a Total-IPL product; c. dissolving said Total-IPL product in a two liquid phase aqueous wash, wherein said liquid phases are separated by a solvent partition, wherein one of said liquid phase comprises glycophytosphingolipids (GSL) and the other said liquid phase is substantially free of GSL; d. filtering and evaporating part of said liquid phase that is substantially free of GSL to obtain a substantially GSL-free IPL product; e. precipitating another part of said liquid phase that is substantially free of GSL, wherein a precipitate is formed comprising phytoglycolipids (PGL) and a supernatant is formed comprising a substantially PGL-free IPL product; f. recovering PGL-free IPL filtrate by filtration or PGL-free IPL supernatant by centrifugation and evaporating said filtrate or supernatant to obtain a substantially PGL-free IPL product; g. adsorbing on silica remaining PGL and GSL from said substantially PGL-free IPL product to obtain a substantially pure PI product; h. eluting said substantially pure PI product with a solvent; i. purifying said substantially PGL-free product by isolating in sodium salt form and subjecting said sodium form product to crystallization; j. Optionally, step (c) by dissolving Total-IPL in CHCl wherein said substantially PGL-free and substantially GSL-free products comprise from about 1% w/w to about 5% w/w of the said PGL or GSL, respectively.