Patent ID: 6348330
Filing Date: 2002-02-19
Classification: C07K

Abstract:
A method for determining the binding of a test sample to the human neurokinin-3 receptor using a Chinese hamster ovarian cell line (CHO), the cell line transplanted with a plasmid, which plasmid comprises:(a) a mammalian expression vector, and (b) a sequence of nucleotides encoding the human neurokinin-3 receptor protein, wherein the sequence comprises a sequence of nucleotides a shown in SEQ ID NO:2:, the method which comprises:(1) expressing human neurokinin-3 receptor in the CHO cells; (2) adding a test sample to a solution containing 125I-eledoisin and the CHO cells; (3) incubating the products of Step 2, wherein the incubation is effective for competitive binding of the 125I-eledoisin and the test sample to the human neurokinin receptor; (4) separating the 125I-eledoisin which is bound to the human neurokinin receptor from the 125I-eledoisin which is not bound; (5) measuring the amount of the 125I-eledoisin which is bound to the human neurokinin-3 receptor; and (6) comparing the amount of the 125I-eledoisin which is bound to the human neurokinin-3 receptor to the amount of 125I-eledoisin which is not bound to determine the binding of the test sample to the human neurokinin-3 receptor.