Patent ID: 6136533
Filing Date: 2000-10-24
Classification: C12Q

Abstract:
A method for detecting a target nucleic acid molecule, comprising:(a) reacting a mixture comprising (i) a target nucleic acid molecule; (ii) a single-stranded nucleic acid probe containing a scissile linkage; (iii) an enzyme capable of cleaving the probe portion of a double-stranded target-probe complex at the scissile linkage; and (iv) ribosomal protein, under conditions and for a time sufficient to allow said target nucleic acid and said probe to hybridize to each other and form a double-stranded target-probe complex, followed by cleavage of the probe and cycling of the target to a new uncleaved probe, such that one or more portions of said cleaved nucleic acid probe are released from said target-probe complex; and(b) determining whether cleaved portions of said nucleic acid probe are produced, thereby detecting the presence of said target nucleic acid.