Patent ID: 9181593
Filing Date: 2015-11-10
Classification: C12Q

Abstract:
1. A method for detecting in a sample a 16S rRNA of A. vaginae or a gene encoding a 16S rRNA of A. vaginae , comprising the steps of: a. providing a sample, wherein said sample is suspected of containing an b. contacting said sample with at least two amplification oligomers, wherein a first amplification oligomer comprises a target hybridizing sequence that consists of SEQ ID NO:2, and wherein a second amplification oligomer comprises a target hybridizing sequence that consists of SEQ ID NO:27, and wherein said first and second amplification oligomers are configured to specifically hybridize to a target sequence within a 16S rRNA of c. performing an in vitro isothermal nucleic acid amplification reaction wherein any target nucleic acid present in said sample is used as a template for generating an amplification product; and d. contacting said in vitro nucleic acid amplification reaction with a detection probe oligomer that comprises a nucleotide sequence consisting of SEQ ID NO:4 or SEQ ID NO:17 and providing conditions for a detection reaction to determine the presence or absence of an amplification product; wherein the detection of the presence of an amplification product indicates the presence of at least 100 CFU/ml of Atopobium vaginae bacterium in the sample.