Patent ID: 7890268
Filing Date: 2011-02-15
Classification: C12Q,G16B

Abstract:
1. A method for the sequencing of a target nucleic acid comprising: a) performing a multistage mass spectrometry, comprising b) comparing the measured CID mass spectrum of the target nucleic acid measured in step v) with a fraction of all calculated CID mass spectra, wherein each of said calculated CID mass spectra correspond to a base sequence having the base composition determined in step iii), wherein the comparison of the measured CID mass spectrum with the calculated CID mass spectra of step b) is performed by an optimization algorithm comprising the evolution steps of: wherein said optimization algorithm compares said measured CID mass spectrum successively with said plurality of calculated CID mass spectra and determines a respective score value for each comparison, said score value representing the degree of consistency between said measured CID mass spectrum and said calculated CID mass spectra, and wherein the base sequence corresponding to the calculated CID mass spectra yielding the best score value is selected as the base sequence of said target nucleic acid, and said base sequence is presented in a user readable format.