Patent ID: 8753841
Filing Date: 2014-06-17
Classification: C12N,C12P

Abstract:
1. A recombinant DNA comprising a DNA encoding a protein selected from the group consisting of (K), (L), (W), and (X), wherein said protein has an amino acid sequence defined as follows: (K) the amino acid sequence consisting of amino acid residues numbers 18 to 644 of SEQ ID NO:27, (L) an amino acid sequence which includes substitution, deletion, insertion, and/or addition, of one to 30 amino acids in the amino acid sequence consisting of amino acid residues numbers 18 to 644 of SEQ ID NO:27, and has at least 50% of the peptide-forming activity of a protein corresponding to unmutated amino acid residue numbers 18 to 644 of SEQ ID NO:27 at 50° C. and a pH of 8, (W) the amino acid sequence consisting of SEQ ID NO:27, and (X) an amino acid sequence which includes substitution, deletion, insertion, and/or addition, of one to 30 amino acids in the amino acid sequence consisting of SEQ ID NO:27, and has at least 50% of the peptide-forming activity of a protein corresponding to unmutated SEQ ID NO:27 at 50° C. and a pH of 8, or an isolated DNA selected from the group consisting of (k), (k2), and (w), wherein said DNA has a nucleotide sequence defined as follows: (k) a nucleotide sequence consisting of nucleotide numbers 104 to 1888 of SEQ ID NO:26, (k2) a nucleotide sequence that hybridizes under stringent conditions with a DNA having a nucleotide sequence complementary to a nucleotide sequence consisting of nucleotide numbers 112 to 1992 of SEQ ID NO:26 under stringent conditions, wherein said stringent conditions are 0.1×SSC and 0.1% SDS at 65° C., and encodes a protein that has at least 50% of the peptide-forming activity at 50° C. and a pH of 8 of a protein encoded by unmutated nucleotide numbers 112 to 1992 of SEQ ID NO:26, (w) a nucleotide sequence consisting of nucleotide numbers 29 to 1888 of SEQ ID NO:26.