Patent ID: 8409861
Filing Date: 2013-04-02
Classification: C07K,C12N

Abstract:
1. A method for deleting sequences in a region of interest in double-stranded DNA of genomic cellular chromatin in a cell, the method comprising: expressing first, second, third and fourth fusion proteins in the cell, each of the fusion proteins comprising: wherein the cleavage half-domain of at least one of the fusion proteins comprises an alteration in amino acid 490 of the wild-type dimerization interface of the cleavage half-domain; and further wherein: (a) the first and second fusion proteins bind to first and second target sites respectively, wherein a first cleavage site lies between the first and second target sites, and (b) the third and fourth fusion proteins bind to third and fourth target sites respectively, wherein a second cleavage site lies between the third and fourth target sites; such that the first and second fusion proteins cleave the DNA at the first cleavage site, the third and fourth fusion proteins cleave the DNA at the second cleavage site, and DNA ends are rejoined such that sequences between the first and second cleavage sites are deleted.