Patent ID: 8809071
Filing Date: 2014-08-19
Classification: B01J,C07K,C08F,C09D,G01N

Abstract:
1. A method for immobilizing biological molecules on to a surface of a substrate, the method consisting of: providing a substrate consisting of a plastic or glass surface; providing an aqueous solution of an N-substituted polyacrylamide polymer obtained by copolymerization of a mixture comprising: (a) a monomer selected from acrylamide and methacrylamide, wherein the acrylamide or methacrylamide is di-substituted on the nitrogen by C1-C12 linear or branched alkyl chains, which in turn can be substituted by a halogen, or by a methoxy; (b) a monomer selected from allyl oxyalkyl(C1-C4) oxiranes, N-acryloyloxy succinimide, N-acryloyloxyalkyl(C1-C4) succinimide, and acryloyl carboxy acids in which the carboxy group is spaced from the acryloyl residue by a C1-C5 alkyl chain; and optionally, (c) an ethylene monomer bearing epoxy functions, sequentially performing the following steps: contacting the surface of the substrate with the aqueous solution of the polyacrylamide polymer for a time sufficient for polymer adsorption to the surface; then adsorbing the polyacrylamide polymer onto the surface to form a coating on the surface; then contacting the polyacrylamide polymer coating with the biological molecules; and then covalently binding the biological molecules to the polyacrylamide polymer; wherein said aqueous solution contains ammonium sulfate.