Patent ID: 8541200
Filing Date: 2013-09-24
Classification: C07K,C12N

Abstract:
1. A method for the preparation of a polypeptide of interest in authentic form, said method comprising the steps of: (i) providing a fusion protein comprising, from its N-terminal to its C-terminal, (a) a fusion partner, (b) a Granzyme B protease recognition site comprising a Granzyme B protease cleavage site that is cleavable by human Granzyme B protease, and wherein the recognition site comprises an amino acid sequence of the general formula  (c) the polypeptide of interest, wherein said cleavage site is adjacent to the polypeptide of interest, and (ii) cleaving the fusion protein with Granzyme B protease at said cleavage site to yield said polypeptide of interest in authentic form.