Patent ID: 7160998
Filing Date: 2007-01-09
Classification: B01L,C12Q,G01N,Y10T

Abstract:
1. A kit for conducting real time monitoring of a PCR amplification of a locus of a nucleic acid sample, said kit comprising: a first oligonucleotide labeled with a first fluorophore at the 3′ end of the first oligonucleotide; and a second oligonucleotide labeled with a second fluorophore, wherein said second oligonucleotide is modified to comprise a terminal 3′ phosphate; wherein the first and second fluorophores comprise a fluorescence resonance energy transfer pair, and the first and second oligonucleotides are configured to hybridize to the locus such that hybridization of the first and second oligonucleotides to the locus, during said PCR, places the first and second fluorophores in a resonance energy transfer relationship.