Patent ID: 6569666
Filing Date: 2003-05-27
Classification: A01K,A61P,C12N,C12Q,Y10S

Abstract:
A method for preparing a sphingosine-1-phosphate lyase, the method comprising culturing a host cell transformed or transfected with an isolated polynucleotide selected from the group consisting of:a. a polynucleotide comprising the sequence set forth in SEQ ID NO:12; b. polynucleotides that hybridize to a polynucleotide completely complementary to the polynucleotide set forth in SEQ ID NO:12 under moderately stringent conditions; and c. a polynucleotide encoding a polypeptide comprising the polypeptide set forth in SEQ ID NO:11, or a portion thereof, that has sphingosine-1-phosphate lyase activity; under conditions promoting expression of the polynucleotide and recovering a sphingosine-1-phosphate lyase.