Patent ID: 9206449
Filing Date: 2015-12-08
Classification: C07C,C12P,C12Y

Abstract:
1. A method for biologically producing p-hydroxybenzoic acid, comprising: contacting a substrate with a biocatalyst, wherein the substrate is a lignin-degradation product that comprises an aromatic carboxylic acid having (a) a p-hydroxy group and (b) a hydroxyl group or C1-C4 alkoxy group attached to at least one other position of the aromatic ring, and the biocatalyst comprises a purified enzyme that removes the hydroxyl group or C1-C4 alkoxy group of (b) to produce p-hydroxybenzoic acid; a recombinant microorganism transformed with an expression vector encoding the enzyme; or a lysate of the recombinant microorganism, or an extract of the recombinant microorganism lysate, comprising the enzyme wherein the enzyme is anthranilate synthase (EC 4.1.3.27), 5-O-(1-carboxyvinyl)-3-phosphoshikimate phosphate-lyase (EC 4.2.3.5), chorismate lyase (EC 4.1.3.40), 3-dehydroshikimate hydrolyase (EC 4.2.1.118), isochorismate lyase (EC 4.2.99.21), aminodeoxychorismate lyase (EC 4.1.3.38), 3-dehydroquinate hydro-lyase (EC 4.2.1.10), prephenate hydro-lyase (EC 4.2.1.51), or hydroxyphenylpyruyate synthase (EC 5.4.99.5).