Patent ID: 7601489
Filing Date: 2009-10-13
Classification: C12Q,G01N

Abstract:
1. A method of identifying an agent that reduces the activity of reverse transcriptase comprising: a) contacting a cell comprising a divalent cation transporting protein with a test agent; b) detecting a change in the concentration of intracellular manganese ions in the cell after contact with the test agent as compared to the concentration of manganese ion levels in the absence of the test agent; and c) detecting reverse transcriptase activity in the cell using a polyribonucleotide or polydeoxyribonucleotide template for reverse transcriptase activity, wherein an increase in the concentration of intracellular manganese ions in the cell after contact with the test agent, and a decrease in activity of reverse transcriptase is indicative of an agent that reduces the activity of reverse transcriptase.