Patent ID: 7541164
Filing Date: 2009-06-02
Classification: C07K,C12N,C12P

Abstract:
1. A cell culturing process comprising: a) culturing host cells which produce soluble cytotoxic T-lymphocyte antigen-4 (CTLA4) molecules at a first temperature of 37° C. under cell culture conditions and for a time period that allows for cell growth; b) then culturing the cells at a second temperature of 34° C; c) then culturing the cells at a third temperature of 32° C. and d) then culturing the cells at a fourth temperature of 30° C. and wherein the cells are cultured at the second temperature starting about day 5 to day 7 of the culture, and wherein there are approximately four days between the start of the second temperature and the start of the third temperature and wherein the fourth temperature starts on or about two weeks from the start of the culture until the end of the culturing process.