Patent ID: 8058006
Filing Date: 2011-11-15
Classification: C12Q

Abstract:
1. A method for obtaining and initiating amplification of a target nucleic acid sequence from a mycobacterial organism present in a sample, the method comprising the steps of: a) exposing a sample to a lytic composition for about 15 minutes to at least, or about, 60 minutes under conditions sufficient to kill and lyse said organism that include a temperature of about 60° C. to at least, or about, 95° C., thereby releasing a target nucleic acid into a lysate, the lytic composition comprising a detergent, an amplification oligonucleotide for amplifying a target nucleic acid sequence contained in the target nucleic acid, a solid support comprising a plurality of magnetically charged particles or beads, and a capture probe for immobilizing the target nucleic acid on the solid support; b) forming a hybrid complex in the lysate, which hybrid complex comprises the capture probe, the target nucleic acid and the amplification oligonucleotide; c) immobilizing the hybrid complex on the solid support and removing components of the lysate which are not part of the hybrid complex formed in step b); and d) exposing the target nucleic acid to amplification conditions, such that the amplification oligonucleotide is enzymatically extended to form a complementary copy of the target nucleic acid sequence.