Patent ID: 6558678
Filing Date: 2003-05-06
Classification: A61K,A61P,Y02A,Y10S

Abstract:
A method for producing a formalin-killed E. coli bacterial strain for use in a vaccine against enteric infection caused by E. coli bacteria in humans comprising the steps of:providing at least one E. coli bacterial strain expressing colonization factor antigens on the surface of said bacteria, growing said E. coli bacterial strain in liquid culture medium with vigorous agitation to a predetermined density, harvesting said E. coli bacterial strain, resuspending said harvested E. coli bacterial strain in saline, adding formalin to said harvested, resuspended bacterial strain to a final concentration of 0.2M formaldehyde, incubating said formalin-treated E. coli bacterial strain at 37Â° C. under conditions of continuous agitation for about 2 hours, further incubating said formalin-treated bacterial strain at 4Â° C. for between about 24 hours and about 48 hours, thereby obtaining a formalin-killed E. coli bacterial strain and, collecting said formalin-killed E. coli bacterial strain.