Patent ID: 7138265
Filing Date: 2006-11-21
Classification: C12N,C12Y,G01N

Abstract:
1. A method for determining the presence or amount of an analyte in a test sample, comprising: forming a mixture of (1) a chimeric enzyme comprising β-lactamase and a binding site moiety, said binding site moiety including at least one amino acid, said chimeric enzyme having a sequence of said binding site moiety inserted in said enzyme or replacing at least one amino acid of said enzyme with the proviso that the activity of the chimeric enzyme is modulated upon binding of a binding molecule to the binding site moiety, (2) a test sample, (3) a binding molecule which binds to a binding site moiety of the chimeric enzyme and modulates the activity of the enzyme, and (4) a substrate upon which the chimeric enzyme catalytically acts; and detecting the amount of catalysis of the substrate and thereby determining the presence or absence of said analyte of interest.