Patent ID: 8105830
Filing Date: 2012-01-31
Classification: A61K,A61P,C07K

Abstract:
1. A method for killing a target cell comprising a major histocompatibility complex (MHC) or human-leukocyte-associated antigen (HLA), the method comprising: a) contacting a plurality of cells comprising immune cells and the target cell with a single-chain polyspecific binding protein comprising at least one single-chain T-cell receptor (sc-TCR) and at least one single-chain Fv (sc-Fv), wherein the at least one sc-TCR specifically binds to a MHC or HLA complexed with a known peptide antigen on the target cell and the at least one sc-Fv specifically binds CD3 on the surface of the immune cells, and wherein said scTCR comprises a V-β chain and a V-α chain covalently linked by a peptide linker and further comprises a C-β chain or fragment thereof fused to the C-terminus of the V-β chain; b) forming a specific binding complex through the single-chain polyspecific binding protein interactions with the known peptide antigen MHC complex or the known peptide antigen HLA complex on the target cells and with the CD3 molecule on the immune cells sufficient to activate the immune cells; and c) killing the target cell with the activated immune cells.