Patent ID: 6639126
Filing Date: 2003-10-28
Classification: C12N,C12Y

Abstract:
A method for modulating polyglucan biosynthesis in a maize plant comprising stably introducing into the genome of said maize plant at least a first DNA construct and a second DNA construct, whereina) said first DNA construct comprises a first nucleic acid molecule operably linked to a first promoter active in said maize plant, wherein expression of said first nucleic acid molecule decreases pullulanase activity of a maize pullulanase polypeptide capable of hydrolyzing &agr;(1â†’6) linkages of pullulan in the maize plant, and said first nucleic acid molecule comprises a nucleotide sequence comprising at least 20 contiguous nucleotides encoding the maize pullulanase polypeptide; and, b) said a second DNA construct comprises a second nucleotide sequence operably linked to a second promoter active in said maize plant wherein expression of said second nucleotide sequence modulates the activity of a polypeptide involved in polyglucan synthesis and said second nucleotide sequence is selected from the group consisting of: i) a nucleic acid molecule comprising a nucleotide sequence that encodes the polypeptide involved in polyglucan synthesis; and, ii) a nucleic acid molecule comprising a nucleotide sequence having an antisense sequence corresponding to the second nucleotide sequence in bi).