Patent ID: 6475775
Filing Date: 2002-11-05
Classification: A61K,C07K,G01N

Abstract:
An isolated polypeptide encoded by an isolated nucleic acid molecule selected from the group consisting of(a) nucleic acid molecules which hybridize under stringent conditions to a molecule consisting of the nucleic acid sequence of SEQ ID NO:1 and which code for a GTPase-activating polypeptide, wherein the stringent conditions are selected from the group consisting of (1) hybridization at 65Â° C. in hybridization buffer (3.5Ã—SSC, 0.02% Ficoll, 0.02% polyvinyl pyrolidone, 0.02% Bovine Serum Albumin, 2.5 mM NaH2PO4 (pH 7), 0.5% SDS, 2 mM EDTA), wherein SSC is 0.15M sodium chloride/0.15M sodium citrate, pH 7, SDS is sodium dodecyl sulphate, and EDTA is ethylenediaminetetracetic acid; and (2) hybridization at 42Â° C. in a hybridization solution containing 50% formamide, 5Ã—SSC (1Ã—SSC is 15 mM sodium citrate and 150 mM sodium chloride), 2Ã—Denhardt's solution, 0.5% SDS, 50 mM sodium phosphate, pH 6.9, and 0.1 mg/ml salmon sperm DNA, (b) nucleic acid molecules that encode the GTPase-activating polypeptide encoded by the nucleic acid molecules of (a), and (c) full length complements of the nucleic acid molecules of (a) or (b).