Patent ID: 7537791
Filing Date: 2009-05-26
Classification: A61K,A61P

Abstract:
1. A method for isolating a bioactive fraction that is derived from cell juice of fresh biomass of a feverfew ( Tanacetum parthenium ) plant and that is substantially free of α-unsaturated γ-lactones, said method comprising: providing fresh biomass of a feverfew ( processing the fresh biomass under conditions effective to yield a cell juice supernatant that is at least substantially free of α-unsaturated γ-lactones and a membrane fraction containing α-unsaturated γ-lactones, said processing the fresh biomass comprising: (i) separating the fresh biomass into a cell juice component and a fiber enriched material; and (ii) subjecting the cell juice component to a combined pH adjustment and microwave treatment step to yield the cell juice supernatant that is at least substantially free of α-unsaturated γ-lactones, wherein the pH adjustment comprises decreasing the pH to a range of 3 to 4; treating the cell juice supernatant under conditions effective to yield a first cell juice serum supernatant and a cytoplasm fraction precipitate; and isolating the cytoplasm fraction precipitate, wherein said cytoplasm fraction precipitate is a bioactive fraction that is substantially free of α-unsaturated γ-lactones, wherein said α-unsaturated γ-lactones comprise parthenolide.