Patent ID: 7405874
Filing Date: 2008-07-29
Classification: G02B

Abstract:
1. A microscope for epi flurorescence and for total internal reflection microscopy, comprising: at least one light source for fluorescence illumination; at least one light source for evanescent illumination; an objective; a beam combiner, wherein the illuminating light coming from the light sources on different illumination paths enters the objective via the beam combiner and is passed to a sample, wherein an exit pupil of the objective is imaged on the beam combiner and the beam combiner is structured such that it guides the illuminating light used for the fluorescence illumination and the illuminating light used for the evanescent illumination into the objective along beam paths which are geometrically separate from one another, an exchange mechanism for the beam combiner configured to bring the beam combiner out of a plurality of beam combiners and into the beam path; and an exchange mechanism for the objective configured to bring the objective out of a plurality of objectives and into the beam path in synchronization with the exchange mechanism for the beam combiner.