Patent ID: 8999657
Filing Date: 2015-04-07
Classification: G01N

Abstract:
1. A method of characterizing and/or treating cancerous tissue comprising: contacting cancerous tissue with a first antibody in a format capable of resolving nuclear binding and cytoplasmic binding, wherein the first antibody is an antibody capable of specifically binding cytoplasmic tail of CD43 in the format; contacting the tissue with a second antibody in the format capable of resolving nuclear binding and cytoplasmic binding, wherein the second antibody is an antibody capable of specifically binding extracellular domain of CD43; and resolving cellular localization of any binding to the tissue with the first antibody and the second antibody, wherein nuclear binding with the first antibody, a lack of cytoplasmic binding with the first antibody, a lack of nuclear binding with the second antibody, and a lack of cytoplasmic binding with the second antibody characterizes the tissue as being more aggressively cancerous than tissue not showing nuclear binding with the first antibody, a lack of cytoplasmic binding with the first antibody, a lack of nuclear binding with the second antibody, and a lack of cytoplasmic binding with the second antibody.