Patent ID: 7384785
Filing Date: 2008-06-10
Classification: C12Q,G01N,Y02A

Abstract:
1. A method for the rapid detection of an anti-WNV antibody specific for WNV NS5 comprising the steps of: (a) contacting a biological sample with a microsphere suspension, each microsphere covalently conjugated via carboxyl groups on its surface to substantially pure recombinant WNV NS5 protein having a native conformation or non-denatured structure whereby each NS5 protein is specifically reactive to antibodies against WNV, (b) incubating the microsphere suspension under conditions sufficient to promote the binding of an anti-WNV antibody to the NS5 protein, (c) contacting the microsphere suspension with a fluorescent reporter antibody, (d) detecting fluorescence intensity of the microsphere suspension using a flow analyzer, wherein detection of fluorescence of the sample indicates the presence of anti-WNV NS5 antibodies in the biological sample; and wherein fluorescence will not be detected in a biological sample from a patient infected with a