Patent ID: 7993828
Filing Date: 2011-08-09
Classification: C12Q

Abstract:
1. A process for DNA amplification by PCR with thermocycling of substances containing the DNA together with associated water-soluble reagents, the process comprising: integrating materials in a closed analysis unit including a single-use cartridge with at least one of microchannels and microcavities, in which at least the water-soluble reagents necessary for the PCR are held in a form which is stable when stored at room temperature, and in which the water-soluble reagents are collectively arranged in a layer; providing a covering layer, consisting essentially of a water-insoluble medium, in the single-use cartridge, wherein the water-insoluble medium is paraffin; covering the layer of water-soluble reagents with the covering layer; supplying the DNA to be amplified in an aqueous solvent, wherein the covering effect of the water-insoluble medium is subsequently negated, so that the water-soluble reagents dissolve in the aqueous solvent; and performing PCR thermocycling reactions on the aqueous solvent including the DNA to be amplified.