Patent ID: 8337861
Filing Date: 2012-12-25
Classification: A61K,A61P,C07K,C12N

Abstract:
1. A method of enhancing the immunogenicity of a bacterial vaccine vector expressing a heterologous antigen, the method comprising the steps of: a) administering to a non-human animal the bacterial vaccine vector; b) passaging the bacterial vaccine vector through the animal; c) harvesting the bacterial vaccine vector from a normal organ or normal tissue in the non-human animal; and d) repeating step a), step b), and step c) with the harvested bacterial vaccine vector until a maximum bacterial load for said vector in said organ or tissue is reached; wherein said maximum bacterial load is reached by the 2 nd passage, wherein said bacterial vector from said maximum bacterial load has reached the maximum bacterial virulence, wherein administering said bacterial vector derived from said maximum bacterial load to a human subject after passaging through said non-human animal results in enhanced immunogenicity of said heterologous antigen in said bacterial vaccine vector, and wherein the bacterial vaccine vector is a Listeria vaccine vector.