Patent ID: 7962316
Filing Date: 2011-06-14
Classification: C12N,G16B

Abstract:
1. A method for selecting from a plurality of different siRNAs one or more siRNAs for silencing a target gene in an organism, each different siRNA in said plurality of different siRNAs targeting a different target sequence in a transcript of said target gene, said method comprising (a) calculating a score for a corresponding targeted sequence motif in said transcript, for each said different siRNA in said plurality of different siRNAs, wherein said score is calculated using a position-specific score matrix (PSSM), wherein each said sequence motif is a nucleotide sequence of L nucleotides, L being an integer, and wherein said PSSM is {log(e (b) ranking said plurality of different siRNAs according to said scores; and (c) selecting one or more siRNAs from said ranked siRNAs, wherein at least one of steps (a), (b) or (c) is performed by a suitably programmed computer.