Patent ID: 7855167
Filing Date: 2010-12-21
Classification: C07K,G01N

Abstract:
1. A method for identifying an interacting set of proteins comprising: A) generating first and second fragments of a fluorescent protein reporter molecule which have a directly fluorescent detectable activity when reconstituted and/or associated, wherein said fluorescent protein is selected from the group consisting of green fluorescent protein and mutants of green fluorescent protein; B) coupling said first fragments of said green fluorescent protein or mutant green fluorescent protein reporter molecule to members of a first panel of proteins; C) coupling said second fragments of said green fluorescent protein or mutant green fluorescent protein reporter molecule to members of a second panel of proteins; D) mixing the products of B) and C); E) directly testing for fluorescence of said green fluorescent protein or mutant green fluorescent protein reporter molecule when reconstituted and/or associated; and F) identifying the protein panel members whose interaction resulted in fluorescence of said green fluorescent protein or mutant green fluorescent protein reporter molecule and which thus form an interacting set.