Patent ID: 6218126
Filing Date: 2001-04-17
Classification: B01J,B01L,C12Q,C40B

Abstract:
A polynucleotide separation method comprising the steps of:(1) immobilizing each of single-stranded oligonucleotide probes each having a different specific base sequence to each of a plurality of independent areas being formed on a surface of a substrate;(2) supplying a sample solution containing polynucleotides labeled with a fluorophore onto said substrate;(3) heating said sample solution to a predetermined temperature;(4) cooling the heated sample solution to hybridize said polynucleotides to said single-stranded oligonucleotide probes;(5) replacing said sample solution on said substrate with a solution containing no polynucleotide;(6) gradually increasing a temperature of the surface of the substrate at one area of the plurality of independent areas and detecting fluorescence emitted from said fluorophore labeling said polynucleotides on said substrate to estimate a temperature of the surface of the substrate at the one area from the intensity of the fluorescence; and(7) denaturing selectively said polynucleotides hybridized to said single-stranded polynucleotide probes by controlling the temperature of the surface of the substrate at the one area according to the estimated temperature, and recovering said denatured polynucleotides thereby separating said polynucleotide.