Patent ID: 6331140
Filing Date: 2001-12-18
Classification: C12N,C12R

Abstract:
A method of using an insertion sequence element as a tool for genetically modifying the genome of Lactobacillus delbrueckii or Lactobacillus helveticus comprising:inserting the insertion sequence element into a bacterial plasmid, wherein the insertion sequence element is selected from DNA sequences of SEQ ID NO: 7, 8, 9, or 10 or a functional derivative thereof, wherein the derivative hybridizes to a complement of SEQ ID: 7, 8, 9, or 10, under very stringent conditions;introducing the plasmid into Lactobacillus delbrueckii or Lactobacillus helveticus;subjecting the Lactobacillus delbrueckii or Lactobacillus helveticus to conditions that inhibit replication of the plasmid; andscreening for genes that were inactivated or subjecting the Lactobacillus delbrueckii or Lactobacillus helveticus to conditions that activate expression of a gene of interest.