Patent ID: 7122369
Filing Date: 2006-10-17
Classification: C12N,C12P,C12R,Y10S

Abstract:
1. A method of producing a raffinate-resistant bacterial strain B which produces a higher amount of L-lysine compared to its parental bacterial strain comprising: (a) selecting a parental bacterial strain A that produces L-lysine; (b) subjecting said parental bacterial strain A to mutagenesis to produce a mutagenized parental bacterial strain A; (c) culturing said mutagenized parental bacterial strain A in a bacterial selection medium containing at least about 1% raffinate based on ammonium sulfate content, wherein said raffinate is the broth effluent waste stream product generated during the ion-exchange chromatographic purification of L-lysine; (d) selecting a raffinate-resistant bacterial strain B from the bacterial selection medium containing said mutagenized parental bacterial strain A, wherein said raffinate-resistant bacterial strain B grows to a higher optical density at a wavelength of 660 nm compared to said parental bacterial strain A and produces a higher amount of L-lysine compared to said parental bacterial strain A.