Patent ID: 6432722
Filing Date: 2002-08-13
Classification: G01N,Y10S,Y10T

Abstract:
A method for the determination of an analyte in a sample by electrochemiluminescence measurement comprising the steps of:(a) bringing the sample and a detection reagent which comprises a metal complex comprising at least one group selected from the group consisting of charge carriers and hydrophilic groups as an electrochemiluminescence marker group coupled to a biological substance capable of binding with said analyte and an electrochemiluminescence cosubstrate into contact with a measuring electrode, said marker group capable of generating a detectable signal, (b) separating the unbound metal complex from the metal complex bound to the analyte, (c) applying a potential to the electrode which enables an electrochemiluminescence reaction to proceed and measuring the signal generated by metal complex bound to analyte during a measurement interval of at least 0.5 seconds, and (d) correlating the measured signal with the presence or amount of the analyte in the sample.