Patent ID: 6270795
Filing Date: 2001-08-07
Classification: A61K,C12N

Abstract:
A method of encapsulating an aqueous solution of DNA in a polymer microparticle, the method comprising:(a) providing an aqueous solution of DNA;(b) providing a solution of polymer in an organic solvent;(c) forming a water-in-oil emulsion containing the aqueous DNA solution in the solution of polymer in organic solvent;(d) combining the water-in-oil emulsion with a second aqueous solution to form a (water-in-oil)-in-water emulsion; and(e) adding the (water-in-oil)-in-water emulsion to excess of a further aqueous phase to extract the oil phase and thereby form a plurality of polymer microparticles;wherein the aqueous solution of DNA has an alcohol content of 1 to 40% and the further aqueous phase is at least 5.degree. C. higher in temperature than the (water-in-oil)-in-water emulsion.