Patent ID: 6284743
Filing Date: 2001-09-04
Classification: A01K,A61K,A61P,C07K,C12N

Abstract:
A method of inhibiting smooth muscle cell proliferation comprising:(a) obtaining an isolated nucleic acid segment comprising a cell cycle regulatory gene operatively linked to an SM22.alpha. promoter, the promoter having a maximum of 441 bases and comprising:(1) SME1 (SEQ ID NO:21), SME2 (SEQ ID NO:23), SME3 (SEQ ID NO:25), SME4 (SEQ ID NO:27), SME5 (SEQ ID NO:31), or SME6 (SEQ ID NO:33), or(2) a sequence that hybridizes with the complement of SME1 (SEQ ID NO:21), SME2 (SEQ ID NO:23), SME3 (SEQ ID NO:25), SME4 (SEQ ID NO:27), SME5 (SEQ ID NO:31), or SME6 (SEQ ID NO:33) under hybridizing conditions comprising 0.02M-0.15M sodium chloride at a temperature of 50.degree. C. to 70.degree. C.;(b) transferring said nucleic acid segment into a smooth muscle cell; and(c) maintaining said smooth muscle cell under conditions effective to express said cell cycle regulatory gene, wherein expression of said cell cycle regulatory gene inhibits proliferation of said smooth muscle cell.