Patent ID: 6355419
Filing Date: 2002-03-12
Classification: C12Q

Abstract:
A method for preparing a plurality of nucleic acid pools comprising:(a) providing a sample of nucleic acids; (b) contacting each nucleic acid with each of a plurality of oligonucleotide probes under conditions for hybridization of oligonucleotide probes to nucleic acids; (c) identifying nucleic acids that hybridize to the same plurality of oligonucleotide probes thereby clustering the nucleic acids in a plurality of groups, wherein each group comprises nucleic acids having tht same or similar nucleotide sequences; (d) determining the number of nucleic acids in each group as an indication of the degree of representation in the sample of the nucleic acids within said group; (e) selecting a single nucleic acid from each group to obtain a series of representative nucleic acids; (f) combining different representative nucleic acids in a plurality of pools based on degree of representation in the sample, wherein the degree of representation of a representative nucleic acid in each pool is sufficiently similar to other different representative nucleic acids in that pool to allow one set of hybridization conditions to be selected for that pool that is suitable for all nucleic acids in that pool.