Patent ID: 7141421
Filing Date: 2006-11-28
Classification: A01K,A61K,A61P,C07K,C12Q,G01N

Abstract:
1. A method for screening for compounds that affect mitochondrial uncoupling, comprising: a) contacting a mammalian cell or tissue sample in vitro with a candidate compound; b) analyzing the contacted mammalian cell or tissue sample for expression of a polypeptide having at least 95% amino acid sequence identity to the polypeptide encoded by SEQ ID NO:2, wherein the polypeptide having at least 95% sequence identity has mitochondrial uncoupling activity; and c) analyzing mitochondrial membrane potential of the contacted mammalian cell or tissue sample, wherein a change in mitochondrial membrane potential and a change in expression of the polypeptide having at least 95% sequence identity, as compared to a control cell not treated with the candidate compound indicates that the compound affects mitochondrial uncoupling.