Patent ID: 8283333
Filing Date: 2012-10-09
Classification: A61K,A61P,C12N

Abstract:
1. A nucleic acid-lipid particle comprising: (a) an siRNA that silences polo-like kinase 1 (PLK-1) gene expression, wherein the siRNA consists of one the following pairs of sense and antisense strand sequences: SEQ ID NOS:3 and 16; SEQ ID NOS:3 and 19; SEQ ID NOS:3 and 21; SEQ ID NOS:3 and 22; SEQ ID NOS:3 and 23; SEQ ID NOS:3 and 24; SEQ ID NOS:3 and 25; SEQ ID NOS:3 and 26; SEQ ID NOS:3 and 27; SEQ ID NOS:3 and 28; SEQ ID NOS:3 and 29; SEQ ID NOS:3 and 30; SEQ ID NOS:3 and 31; SEQ ID NOS:3 and 32; SEQ ID NOS:3 and 33; SEQ ID NOS:4 and 16; SEQ ID NOS:4 and 19; SEQ ID NOS:4 and 20; SEQ ID NOS:4 and 21; SEQ ID NOS:4 and 22; SEQ ID NOS:4 and 23; SEQ ID NOS:4 and 24; SEQ ID NOS:4 and 25; SEQ ID NOS:4 and 26; SEQ ID NOS:4 and 27; SEQ ID NOS:4 and 28; SEQ ID NOS:4 and 29; SEQ ID NOS:4 and 30; SEQ ID NOS:4 and 31; SEQ ID NOS:4 and 32; SEQ ID NOS:4 and 33; SEQ ID NOS:9 and 16; SEQ ID NOS:9 and 19; SEQ ID NOS:9 and 20; SEQ ID NOS:9 and 21; SEQ ID NOS:9 and 22; SEQ ID NOS:9 and 23; SEQ ID NOS:9 and 24; SEQ ID NOS:9 and 25; SEQ ID NOS:9 and 26; SEQ ID NOS:9 and 27; SEQ ID NOS:9 and 28; SEQ ID NOS:9 and 29; SEQ ID NOS:9 and 30; SEQ ID NOS:9 and 31; SEQ ID NOS:9 and 32; SEQ ID NOS:9 and 33; SEQ ID NOS:10 and 16; SEQ ID NOS:10 and 19; SEQ ID NOS:10 and 20; SEQ ID NOS:10 and 21; SEQ ID NOS:10 and 22; SEQ ID NOS:10 and 23; SEQ ID NOS:10 and 24; SEQ ID NOS:10 and 25; SEQ ID NOS:10 and 26; SEQ ID NOS:10 and 27; SEQ ID NOS:10 and 28; SEQ ID NOS:10 and 29; SEQ ID NOS:10 and 30; SEQ ID NOS:10 and 31; SEQ ID NOS:10 and 32; and SEQ ID NOS:10 and 33; (b) a cationic lipid comprising from about 50 mol % to about 65 mol % of the total lipid present in the particle; (c) a non-cationic lipid comprising from about 25 mol % to about 45 mol % of the total lipid present in the particle; and (d) a conjugated lipid that inhibits aggregation of particles comprising from about 5 mol % to about 10 mol % of the total lipid present in the particle.