Patent ID: 7470543
Filing Date: 2008-12-30
Classification: G01N,G01R,Y10T

Abstract:
1. A method of identifying a ligand to a target molecule, the method comprising: providing a reference compound that binds to a target molecule with a binding affinity in the micromolar range; collecting a 1D providing a test sample, the test sample comprising at least one competitive test compound; collecting a 1D comparing the spectrum of the solution of the reference compound in the presence of the target molecule to the spectrum of the solution of the reference compound in the presence of the test sample and the target molecule to determine a change in one or more of the reference compound resonances, wherein the change in one or more of the reference compound resonances comprises an increase in signal intensity in at least one reference resonance; identifying the at least one competitive test compound that interacts with the target molecule based on the change in one or more of the reference compound resonances, wherein the at least one competitive test compound displaces the reference compound; calculating a ratio of target molecule bound reference compound concentration [EL] to total reference compound concentration [L where K D is a separately measured dissociation constant of the reference compound and the target molecule and [E TOT ] is a total concentration of the target molecule; correlating a measured parameter within each 1D determining a value for the 1D determining an apparent dissociation constant for the particular target molecule bound reference compound concentration in the presence of the test compound using the equation, and determining the dissociation constant of the competitive test compound K