Patent ID: 8673602
Filing Date: 2014-03-18
Classification: C07K,C12N,C12P

Abstract:
1. A recombinant bacterium comprising in its genome: (a) one or more nucleotide sequences encoding a polypeptide or a polypeptide complex having sucrose transporter activity, wherein the polypeptide or the polypeptide complex having sucrose transporter activity has (i) at least 95% sequence identity based on the Clustal W method of alignment to an amino acid sequence set forth in SEQ ID NO:24, SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:94, SEQ ID NO:96, SEQ ID NO:98, SEQ ID NO:100, SEQ ID NO:102, SEQ ID NO:104, SEQ ID NO:106, SEQ ID NO:108, or SEQ ID NO:110 or (ii) polypeptide subunits having at least 95% sequence identity, based on the Clustal W method of alignment, to the amino acid sequences set forth in SEQ ID NOs: 30, 32, and 34 or the amino acid sequences set forth in SEQ ID NOs:36, 38, 40, and 42; (b) a nucleotide sequence encoding a polypeptide having fructokinase activity, wherein the polypeptide having fructokinase activity is classified as EC 2.7.1.4, EC 2.7.1.3, or EC 2.7.1.1; and (c) a nucleotide sequence encoding a polypeptide having sucrose hydrolase activity, wherein the polypeptide having sucrose hydrolase activity is classified as EC 3.2.1.26 or EC 2.4.1.7; wherein: (i) (a), (b) and (c) are each operably linked to the same or a different promoter; (ii) (a), (b), and (c) are integrated into the genome between the yihP gene or its homolog and the yihO gene or its homolog; and (iii) said bacterium metabolizes sucrose at a greater rate than a bacterium comprising (a), (b), and (c) integrated at a different location in the genome.