Patent ID: 7919265
Filing Date: 2011-04-05
Classification: C12N,G01N,Y10T

Abstract:
1. A method for isolating Th2 lymphocytes from a mixture of human peripheral blood mononuclear cells (PBMCs), comprising the steps of: (i) contacting said mixture with anti-Th1 monoclonal antibodies comprising anti-CD29-biotin and anti-CXCR3-biotin clones, (ii) contacting said mixture with antibodies that bind to cell surface antigens of non-Th2 leucocytes, wherein the cell surface antigens of the non-Th2 leucocytes comprises CD8, CD14, CD16, CD19, and CD56; (iii) contacting said mixture with an antibody to an erythrocyte cell surface antigen; (iv) contacting the mixture in step in with a magnetic colloid that binds to the antibodies in steps (i) to (iii) to magnetically label the Th1 lymphocytes, non-Th2 leukocytes, and erythrocytes; and (v) separating the magnetically labeled cells from the Th2 lymphocytes with a magnet to obtain isolated Th2 lymphocytes.