Patent ID: 6551781
Filing Date: 2003-04-22
Classification: C07K,C12N

Abstract:
A method of screening for compounds that regulate the level of Nramp, wherein Nramp is a transmembrane protein which participates in substrate translocation across a cellular membrane, and wherein Nramp confers resistance to infection to unrelated intracellular parasites,wherein said method comprises expressing a nucleic acid sequence encoding Nramp in a eukaryotic or prokaryotic host cell, and monitoring changes in Nramp level upon treatment with said compound, wherein said Nramp comprises an amino acid sequence selected from the group consisting of: a) SEQ ID NO:2; b) SEQ ID NO:4; c) SEQ ID NO:6; d) SEQ ID NO:25; e) SEQ ID NO:27; f) SEQ ID NO:28; g) SEQ ID NO:30; h) SEQ ID NO:32; i) SEQ ID NO:33; and j) an amino acid sequence which is at least 93% homologous to one of the amino acid sequences in a) to i); and wherein a compound that regulates Nramp level is identified when said Nramp level is measurably different in the presence of said compound as compared to in the absence thereof.