Patent ID: 6607898
Filing Date: 2003-08-19
Classification: C12Q,Y10T

Abstract:
A method for detecting extracellular human telomerase RNA template (hTR) RNA in blood plasma or serum for detecting, diagnosing, monitoring, treating or evaluating neoplastic disease comprising cells that express hTR, the method comprising the steps of:a) extracting mammalian, heterogeneous extracellular RNA from human blood plasma or serum; b) amplifying a portion of the extracted extracellular RNA or cDNA produced therefrom wherein said portion comprises hTR RNA to produce an amplified product, and wherein amplification is performed qualitatively or quantitatively using primers or probes that target hTR RNA or the corresponding cDNA; and c) detecting qualitatively or quantitatively the amplified product produced from hTR RNA or corresponding cDNA.