Patent ID: 6280929
Filing Date: 2001-08-28
Classification: C12Q

Abstract:
A method of staining target chromosomal DNA to detect in an interphase cell one or more genetic translocations identified with chromosomal abnormalities, said method comprising:(a) providing a heterogeneous mixture of two or more nucleic acid probes having a combined complexity of at least 40 kb, which probes contain nucleic acid segments which are substantially complementary to nucleic acid segments that flank and/or extend partially or fully across breakpoint regions known to be associated with genetic translocations, wherein each probe comprises a distinct fluorescent label;(b) reacting the heterogeneous mixture with the targeted chromosomal DNA by in situ hybridization; and(c) observing the proximity or overlap of the regions stained by each probe, to determine whether said translocation is present in the interphase cell.