Patent ID: 8865410
Filing Date: 2014-10-21
Classification: C12N,C12Q,C40B

Abstract:
1. A method for detecting errors occurring in the preparation and/or sequencing of a DNA sequencing library, the method comprising: (a) incorporating at least one first nucleic acid adaptor molecule into at least one member of a target library comprising a plurality of nucleic acid molecules, wherein the first adaptor molecule comprises a first defined sequence; (b) amplifying the plurality of nucleic acid molecules to produce an input library comprising a first plurality of amplified DNA molecules, wherein the amplified molecules comprise a sequence identical to or complementary to at least a portion of the first adaptor molecule and sequence identical to or complementary to at least a portion of the at least one member of the target library; (c) sequencing at least a portion of the plurality of amplified DNA molecules to produce a plurality of sequencing reads corresponding to the at least one member of the target library; (d) grouping the plurality of sequencing reads that correspond to the at least one member of the target library; and (e) detecting whether an error exists at a nucleotide position, wherein an error exists when variation of nucleotide identity exists among the grouped sequencing reads at a position corresponding to a nucleotide in the at least one member of the target library.