Patent ID: 8703459
Filing Date: 2014-04-22
Classification: C07H,C12N

Abstract:
1. A purified and isolated catalytic domain from a β(1,4)-galactosyltransferase I corresponding to SEQ ID NO: 6, and consisting of a conservative amino acid exchange at amino acid position 344 of SEQ ID NO: 6, wherein the amino acid exchange at position 344 of SEQ ID NO: 6 is selected from M344H, M344E, M344A, M344S, or M344Q, and wherein the catalytic domain catalyzes formation of galactose-β(1,4)-N-acetylglucosamine bond in the presence of magnesium, and further comprising a conservative amino acid exchange at an amino acid position corresponding to amino acid position 342 of SEQ ID NO: 6.