Patent ID: 8815585
Filing Date: 2014-08-26
Classification: C12M,C12N

Abstract:
1. A method for automated expansion of pluripotent stem cells comprising: (a) obtaining a first population of human pluripotent cells in a growth media; (b) separating the pluripotent cells with an automated separation system; (c) delivering the separated cells in fresh growth media into a plurality of culture wells; (d) culturing the pluripotent cells in the growth media in the plurality of culture wells; and (e) repeating steps b-d one or more times to provide an expanded population of pluripotent cells; wherein said culturing does not comprise culturing the pluripotent cells in the presence of fibroblast feeder cells or serum and further wherein the automated separation system comprises contacting the first population of pluripotent cells with a proteolytic enzyme, wherein the proteolytic enzyme is trypsin, recombinant trypsin, a trypsin-like protease or TRYPLE.