Patent ID: 6458552
Filing Date: 2002-10-01
Classification: C07K,C12N,G01N

Abstract:
An in vitro method to detect inhibition of enzymatic activity of metalloproteases comprising:a) combining enzyme protein selected from the group consisting of ADAM8, ADAM15 and MDC-L, and a peptide substrate comprising a detectable label wherein said substrate is selected from the group of amino acid sequences consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11 in the presence or absence of a test compound; b) incubating the reaction mixture of (a) for sufficient time to produce a detectable product as a result of enzymatic activity upon the substrate; and c) measuring the quantity of the product formed in the presence of the tested compound and in its absence, whereby the inhibition is detected when the amount of product formed in the presence of the test compound is less than that in the absence of said test compound.