Patent ID: 6455253
Filing Date: 2002-09-24
Classification: A61K,C07K,C12N,C12Q,C40B,G01N

Abstract:
A method for evolving a polypeptide to comprise an improved serum half-life or stability, the polypeptide being encoded by a DNA substrate molecule, the method comprising:(a) providing a library of variants of the DNA substrate molecule; (b) transforming the variants of (a) into host cells; (c) expressing the variants of (b) to provide polypeptide expression products; (d) screening or selecting from the polypeptide expression products of (c) an evolved polypeptide comprising the improved serum half-life or stability relative to the polypeptide encoded by the DNA substrate molecule in vivo; (e) recovering an evolved DNA substrate molecule that encodes the evolved polypeptide of (d); and, (f) expressing the evolved DNA substrate molecule, thereby providing the evolved polypeptide comprising the improved serum half-life or stability.