Patent ID: 8445666
Filing Date: 2013-05-21
Classification: C12N,G16B

Abstract:
1. A method for post-transcriptionally repressing the expression of a target gene, the method comprising: inserting into the target gene a target sequence capable of specifically hybridizing with SEQ ID NO: 160280 or SEQ ID NO: 160281, allowing the target gene to be transcribed such that the transcript contains the target sequence capable of specifically hybridizing with SEQ ID NO: 160280 or SEQ ID NO: 160281, providing a nucleic acid molecule comprising a precursor sequence, SEQ ID NO: 46984, which precursor sequence comprises mature sequences SEQ ID NO: 160280 and SEQ ID NO: 160281, or providing a nucleic acid molecule comprising one or both of mature sequences SEQ ID NO: 160280 and SEQ ID NO: 160281, and allowing the precursor sequence or the nucleic acid comprising one or both mature sequences to be transcribed and processed to generate one or more interfering ribonucleic acids that comprise the RNA form of mature sequence SEQ ID NO: 160280 and/or the RNA form of mature sequence SEQ ID NO: 160281, such that the one or more interfering ribonucleic acid is present in the same environment as the transcript containing the target sequence, and the expression of the target gene is post-transcriptionally repressed.