Patent ID: 9109237
Filing Date: 2015-08-18
Classification: C12N,C12P,C12Y

Abstract:
1. A method for preparing piceatannol, the method comprising reacting at least one mutant of CYP102A1 with resveratrol, wherein CYP102A1 is a cytochrome P450 from B. megaterium , wherein the mutant of CYP102A1 comprises an amino acid sequence at least 95% identical to SEQ ID NO: 16, wherein the amino acid sequence of the mutant of CYP102A1 differs from SEQ ID NO: 16 by one or more amino acid substitutions at positions 47, 51, 64, 74, 81, 86, 87, 143, 188 and/or 267 of SEQ ID NO: 16, wherein the mutant of CYP102A1 catalyzes the conversion of resveratrol to piceatannol, and wherein the one or more substitutions are selected from the group consisting of: substitution of the amino acid corresponding to arginine at position 47 of SEQ ID NO: 16 with one amino acid selected from the group consisting of alanine, valine, leucine, isoleucine, proline, methionine, phenylalanine, and tryptophan, substitution of the amino acid corresponding to tyrosine at position 51 of SEQ ID NO: 16 with one amino acid selected from the group consisting of phenylalanine, alanine, valine, leucine, isoleucine, proline, methionine, and tryptophan, substitution of the amino acid corresponding to glutamic acid at position 64 of SEQ ID NO: 16 with one amino acid selected from the group consisting of glycine, serine, threonine, cysteine, tyrosine, asparagine, and glutamine, substitution of the amino acid corresponding to alanine at position 74 of SEQ ID NO: 16 with one amino acid selected from the group consisting of glycine, serine, threonine, cysteine, tyrosine, asparagine, and glutamine, substitution of the amino acid corresponding to phenylalanine at position 81 of SEQ ID NO: 16 with one amino acid selected from the group consisting of alanine, valine, leucine, isoleucine, proline, methionine, and tryptophan, substitution of the amino acid corresponding to leucine at position 86 of SEQ ID NO: 16 with one amino acid selected from the group consisting of alanine, valine, isoleucine, proline, methionine, phenylalanine, and tryptophan, substitution of the amino acid corresponding to phenylalanine at position 87 of SEQ ID NO: 16 with one amino acid selected from the group consisting of alanine, valine, leucine, isoleucine, proline, methionine, and tryptophan, substitution of the amino acid corresponding to glutamic acid at position 143 of SEQ ID NO: 16 with one amino acid selected from the group consisting of glycine, serine, threonine, cysteine, tyrosine, asparagine, and glutamine, substitution of the amino acid corresponding to leucine at position 188 of SEQ ID NO: 16 with one amino acid selected from the group consisting of glycine, serine, threonine, cysteine, tyrosine, asparagine, and glutamine, and substitution of the amino acid corresponding to glutamic acid at position 267 of SEQ ID NO: 16 with one amino acid selected from the group consisting of alanine, valine, leucine, isoleucine, proline, methionine, phenylalanine, and tryptophan.