Patent ID: 9084830
Filing Date: 2015-07-21
Classification: A61K,A61P,B82Y,C07K,Y10S

Abstract:
1. A method of making a pharmaceutical composition, the method comprising: providing bacteria comprising a cell wall, an antigenic protein and an extracellular domain; removing the cell wall from the bacteria to provide a cell-wall-deficient composition that is deficient of the cell wall and comprises the antigenic protein and the extracellular domain; processing the cell-wall-deficient composition to produce microvesicles comprising the antigenic protein and the extracellular domain, wherein the resulting microvesicles comprise a mixture of first type microvesicles with the extracellular domain on their outer surfaces and second type microvesicles with the extracellular domain on their inner surfaces; isolating, from the mixture, at least part of the first type microvesicles with the extracellular domain on their outer surfaces; and formulating a pharmaceutical composition comprising the isolated microvesicles with the extracellular domain on their outer surfaces.