Patent ID: 8114633
Filing Date: 2012-02-14
Classification: G01N,Y10S,Y10T

Abstract:
1. A method to quantitate the amount of recombinant protein (rP) in a sample containing both plasma-derived protein (pdP) and recombinant protein (rP) from an individual, wherein the plasma-derived protein and recombinant protein are the same protein having different glycosylation patterns, said different glycosylation patterns giving rise to different degrees of binding to lectin, wherein the combined amount of plasma-derived protein and recombinant protein is equal to total protein (pdP+rP=tP), said total protein being pre-determined, said method comprising the steps of: (a) contacting the sample from the individual with a lectin composition and detecting differential protein binding of the plasma-derived protein and recombinant protein to the lectin composition, (b) calculating a difference between lectin binding to the total protein in said sample from said individual and expected lectin binding to a total protein in a reference sample of equal volume having an amount of the plasma-derived protein equal to the total protein (tP) in the sample from the individual, wherein the difference in lectin binding correlates to the amount of recombinant protein in the sample from the individual.