Patent ID: 6972198
Filing Date: 2005-12-06
Classification: G01N,Y10S,Y10T

Abstract:
1. A method for determining the conformational state of a protein, comprising the steps of: a) contacting a protein with a labeled first binding partner which binds to said protein in a manner dependent on the conformational state of said protein and which generates a signal in a manner dependent on the binding of the first binding partner to the protein, and a labeled second binding partner which binds to said protein in a manner independent of the conformational state of said protein and which generates a signal in a manner dependent on the binding of the first binding partner to the protein, wherein said protein and said labeled first and second binding partner are not covalently bound; and b) detecting said protein by the binding of at least one of said labeled first binding partner and said labeled second binding partner to said protein wherein detection of a signal generated by said labeled first binding partner and/or said labeled second binding partner is an indicator of the conformational state of said protein.