Patent ID: 8518699
Filing Date: 2013-08-27
Classification: C07K,C12N

Abstract:
1. A method for producing a hypermutated transgenic target nucleic acid sequence comprising a) transfecting a chicken immunoglobulin expressing B lymphoid cell with a genetic construct comprising a target nucleic acid sequence flanked by nucleic acid sequences homologous to an endogenous V gene immunoglobulin locus; b) integrating said genetic construct comprising said target nucleic acid sequence into said endogenous V gene immunoglobulin locus of said chicken immunoglobulin expressing B lymphoid cell, wherein said chicken immunoglobulin expressing B lymphoid cell comprises a functional activation-induced deaminase (AID) protein, and functional XRCC2, XRCC3, and RAD51 proteins or their analogues; and c) culturing said chicken immunoglobulin expressing B lymphoid cell transgenic for said target nucleic acid to produce said hypermutated transgenic target nucleic acid sequence.