Patent ID: 6063630
Filing Date: 2000-05-16
Classification: A61K

Abstract:
A method of producing a clonal cell strain of homologously recombinant secondary somatic non-imnmortalized cells of vertebrate origin selected from the group consisting of mammalian secondary fibroblasts, keratinocytes, epithelial cells, endothelial cells, glial cells, neural cells, lymphocytes, bone marrow cells, muscle cells, hepatocytes and precursors thereof, said method comprising the steps of:a) transfecting a primary or secondary somatic non-imnmortalized cell of vertebrate origin with a DNA construct comprising exogenous DNA which includes a DNA sequence homologous to a genomic DNA sequence of the primary or secondary cell, thereby producing a transfected primary or secondary cell, and wherein said exogenous DNA further comprises a DNA sequence that encodes a therapeutic product selected from the group consisting of enzymes, cytokines, hormones, antigens, antibodies, clotting factors, regulatory proteins, transcription proteins, and receptors;b) maintaining the transfected primary or secondary cell under conditions appropriate for homologous recombination between a DNA sequence in the DNA construct and genomic DNA to occur, thereby producing a homologously recombinant primary or secondary cell; andc) culturing the homologously recombinant primary or secondary cell under conditions appropriate for propagating the homologously recombinant primary or secondary cell, thereby producing a clonal cell strain of homologously recombinant secondary somatic non-imnmortalized cells, wherein the clonal cell strain supplies said therapeutic product.