Patent ID: 8697605
Filing Date: 2014-04-15
Classification: B01J,C12Q,C40B

Abstract:
1. An array of DNA molecules, comprising: a support structure that has a surface wherein the surface comprises regions and the regions comprise a plurality of DNA polymers that are attached to the surface of the support structure; wherein at least one of the regions comprises a first DNA polymer that comprises a first known sequence and a second DNA polymer that comprises a second known sequence and the first known sequence is different from the second known sequence and wherein the first known DNA sequence is complementary to the second known DNA sequence, wherein the first DNA polymer and the second DNA polymer are attached to the substrate surface through one trifunctional linker molecule; wherein the trifunctional linker molecule has three distinct sites for attachment and wherein the first DNA polymer is attached to one of the three distinct sites, the second DNA polymer is attached to a second of the three distinct sites, and a third site is attached to the surface of the substrate; wherein the first DNA polymer is terminated with a group that prevents further polymer synthesis under DNA synthesis conditions; and wherein the at least one region consists essentially of the copies of the first DNA polymer and the second DNA polymer.