Patent ID: 7294457
Filing Date: 2007-11-13
Classification: C12Q,G01N

Abstract:
1. A method for identifying compounds binding to Hepatitis C Virus polymerase comprising the steps of: a) contacting said Hepatitis C Virus polymerase with a probe being capable of binding to said Hepatitis C Virus polymerase, so as to form a complex comprising said probe bound to said polymerase; b) measuring a signal emitted from said probe in said complex to establish a base line level: c) incubating said complex from step a) with a test compound; d) measuring said signal from said complex from step c); and e) comparing said signal from step d) with said signal from step b) wherein said probe is comprised of a compound according to general formula Ia: wherein R or R R R Z is OR or Z is N(R R or R wherein, preferably, R 7 and R 8 are each independently H, (C 1-6 )alkyl, haloalkyl, (C 3-7 )cycloalkyl, 6- or 10-membered aryl, Het, (C 1-6 )alkyl-aryl, (C 1-6 )alkyl-Het, wherein said alkyl, cycloalkyl, aryl, Het, (C 1-6 )alkyl-aryl, (C 1-6 )alkyl-Het are optionally substituted with R 70 ; or R wherein R Y R Q is a 6- or 10-membered aryl, Het, all of which being optionally substituted with: wherein R or an enantiomer, diastereomer or tautomer thereof, including a salt thereof; wherein said probe comprises a detectable label attached to any suitable position, whereby said probe binds to an HCV polymerase and is capable of being displaced by an inhibitor thereof and whereby a modulation in said signal is an indication that said test compound binds to said polymerase.