Patent ID: 8785210
Filing Date: 2014-07-22
Classification: B01L,G01N

Abstract:
1. Test kit based on chromatographic test strips for the detection of a known hapten-antihapten complex, comprising a plurality of test strips each comprising a strip-shaped carrier coated with a thin layer of a chromatographic separation material comprising a labelling zone impregnated with mobile reporter molecule-labelled antibodies or receptors against a first hapten; a detection zone, in which a receptor against a hapten has been immobilised; and a control zone, in which a second receptor against the mobile reporter molecule-labelled antibody or receptor has been immobilised; in combination with one or more first and second vessels for receiving and positioning said test strips, said first and second vessels each containing first and second hapten-coupled receptors against the analyte for the formation of a hapten-antihapten complex, wherein each first vessel further comprises a known amount of analyte, embedded in a glass-like trehalose layer which is dried onto an interior wall of the first vessel as a layer in such a way that the layer dissolves in an aqueous sample solution during reaction of the sample with the hapten-coupled receptors, such that in the first vessel there is always a pre-determined amount of detection complex, which becomes visible during chromatographic analysis of the test strip and provides a control for the sample work-up, the complex formation and the chromatographic analysis, and wherein the second vessels do not comprise a known amount of analyte, wherein the known amount of analyte in the first vessel corresponds to a required detection limit, which is visible as a hapten-antihapten complex on the test strip, and wherein the first and second vessels comprise pre-determined amounts of hapten-coupled receptors against the analyte, which are dried in such a way that they dissolve upon addition of the aqueous sample.