Patent ID: 8906639
Filing Date: 2014-12-09
Classification: C12Q,G01N

Abstract:
1. A method for the quantitative determination of factor XIII content in a plasma sample, comprising: mixing the plasma sample with a substance or substance mixture for activating fact XIII to factor XIIIa, measuring the transglutaminase activity of the activated factor XIIIa via the oxidative consumption of an NAD(P)H analog in the test mixture, wherein the NAD(P)H analog is thio-NAD(P)H or seleno-NAD(P)H, determining one or more reference values for factor XIIIa activity using one or more reference materials having a reduced factor XIIIa activity compared to the norm value of 100%, wherein each of such one or more reference materials consists of plasma, and comparing the measured transglutaminase activity of the activated factor XIIIa in the plasma sample to at least the one or more reference values determined from the one or more reference materials having the reduced factor XIIIa activity compared to the norm value of 100% to determine the factor XIII content in the plasma sample.