Patent ID: 9096688
Filing Date: 2015-08-04
Classification: A61F,A61L,C07K

Abstract:
1. A method of producing a collagen-containing membrane comprising the steps of: (i) isolating a collagen-containing tissue and incubating the isolated collagen-containing tissue in an ethanol solution; (ii) incubating the isolated collagen-containing tissue from step (i) in a solution comprising about 1% (v/v) SDS and about 0.2% (v/v) LiCl in order to denature non-collagenous proteins contained therein; (iii) incubating the collagen-containing tissue produced in step (ii) in a solution comprising about 0.5% (v/v) HCl until the collagen in said material is denatured; and (iv) incubating the collagen-containing tissue produced in step (iii) in a solution comprising about 1% (v/v) HCl with simultaneous mechanical stimulation for sufficient time to enable the collagen bundles in said collagen-containing tissue to align; wherein the mechanical stimulation comprises applying tension cyclically to the collagen-containing tissue, thereby producing said collagen-containing membrane.