Patent ID: 7829323
Filing Date: 2010-11-09
Classification: B01F,C12M,C12N

Abstract:
1. A lysis process comprising the steps of: providing a flow of lysis solution in a first direction; providing a flow of one or more additional fluids in a direction other than the direction of the flow of the lysis solution; wherein the lysis solution and the cells are subjected to an intense shearing during a short time period of less than 10 seconds to mix the lysis solution and the cells and to lyse the cells without a permanent denaturing of the biological substances compounds of interest; wherein the biological substances compounds of interest include proteins, RNA, DNA, viruses, phages, and virus-like particles; passing a combined fluid that results from the lysis solution being mixed with the one or more additional fluids comprising the biological cells through a vortex reducer to reduce or suppress any rotational velocity or any toric component of the flow and to obtain a laminar flow.