Patent ID: 8211668
Filing Date: 2012-07-03
Classification: C12M,C12N,C12P

Abstract:
1. A method of preparing a recombinant polypeptide of interest, comprising: fermenting in a fermentation medium a host cell transformed with a recombinant expression system where a polypeptide of interest is secreted into the periplasm of the cell, and wherein a fermentation broth is formed; providing a solution; admixing the solution with the cell to form a high molarity slurry; providing a low molarity shock buffer, wherein the osmolarity of the high molarity slurry is higher than the osmolarity of the low molarity shock buffer; generating a first fluid stream comprising the high molarity slurry, wherein the first fluid stream has a first flow rate; generating a second fluid stream comprising the low molarity shock buffer, wherein the second fluid stream has a second flow rate; continuously combining the first and second fluid streams into a third fluid stream, wherein the second flow rate is greater than the first flow rate; and extracting the polypeptide of interest from the third fluid stream, wherein a polypeptide extract having at least 70% of the polypeptide of interest compared to the fomentation broth is formed.