Patent ID: 6716593
Filing Date: 2004-04-06
Classification: C12Q,G01N,Y10S

Abstract:
A method for measuring a level of pyridinoline and/or deoxypyridinoline crosslinks in a fluid sample, said method comprisingproviding a fluid sample that contains one or more peptide-bound collagen pyridinium crosslinks selected from the group consisting of pyridinoline, deoxypyridinoline, or both, contacting the sample with a protease reagent under conditions effective for the protease reagent to cleave said crosslinks from one or more attached collagen amino acids and peptides, to form native, peptide-free pyridinium crosslinks and after said contacting, measuring a level of said native, peptide-free pyridinium crosslinks selected from the group consisting of native, peptide-free pyridinoline and/or native, peptide-free deoxypyriinoline, in the sample.