Patent ID: 6221662
Filing Date: 2001-04-24
Classification: C12N

Abstract:
A method of generating cellular models demonstrating Alzheimer's-like characteristics comprising:a) selecting a human neuroblast cell line including epithelial cells,b) subjecting the neuroblast cell line to a solution of trypsin and thereby washing out the neuroblast cells from the epithelial cells in order to segregate the epithelial cells from neuroblast cells in the neuroblast cell linec) cultivating the epithelial cells,d) isolating the epithelial cells,e) separating out the isolated epithelial cells, and acutely treating the separated portion of the epithelial cells with tryptamine to define a grouping of epithelial cells,f) treating the grouping of epithelial cells with generally about 50 to 100 micrograms/milliliters of tryptamine in a culture medium for a period of up to 60 days, andg) generating new neurofibrillary tangles and changes of cytoskeleton associated tryptophanyl-tRNA synthetase (TrpRS) within the grouping of epithelial cells.