Patent ID: 8216573
Filing Date: 2012-07-10
Classification: C07K

Abstract:
1. A method for producing a correctly folded and assembled full-length antibody using an E. coli strain, the method comprising: a) fermenting in a culture medium an E. coli strain which leaks periplasmic proteins into a medium, the E. coli strain comprising: a gene coding for a heavy chain of an antibody functionally linked to a signal sequence coding for a signal peptide; and a second gene coding for the light chain of an antibody, functionally linked to a signal sequence coding for a signal peptide, wherein the E. coli strain secretes a full-length antibody into a culture medium and wherein the leaky mutant is an E. coli strain selected from the group consisting of JF733, A592, A593, A586, CAG12184, G11e1, JE5511, E610, E623, JE5505, PM61, 6628, PM61R, JP1228, 207, and AE84064; and b) removing the full-length antibody from the culture medium.