Patent ID: 7346458
Filing Date: 2008-03-18
Classification: C12Q,G16B

Abstract:
1. A genotyping method comprising hybridizing a target nucleic acid to a DNA chip on which an optimal probe pair is immobilized, wherein the optimal probe pair consists of a wild type-perfect match probe for a mutation site on the target nucleic acid and a mutant type-perfect match probe for the mutation site, wherein the optimal probe pair is selected by: designing a plurality of probe pairs for the mutation site, wherein a probe pair consists of a wild type-perfect match probe and a mutant type-perfect match probe; immobilizing the plurality of probe pairs on a substrate to manufacture an optimal probe pair screening chip; hybridizing a standard nucleic acid to the optimal probe pair screening chip; collecting quantitative hybridization intensity data; calculating a value for each probe pair of the following equation; wherein N denotes a number of times hybridization of the standard nucleic acid has been performed; r r Mean and SD denote a mean value and a standard deviation, respectively, of N In(r) values, which are obtained by hybridizing the standard nucleic acid to the DNA chip N times; and selecting the probe pair having the largest value as the optimal probe pair.