Patent ID: 6812379
Filing Date: 2004-11-02
Classification: C07K,C12N

Abstract:
A method for producing a non-methionine N-terminus protein in a plastid, wherein said method comprises:transforming a plastid with a construct comprising, as operably joined components in the 5â€² to 3â€² direction of transcription, (a) a promoter functional in a plastid, (b) a DNA sequence encoding a cleavable ubiquitin peptide, (c) a DNA sequence encoding a protein of interest, (d) a transcription termination region; (e) at least two DNA regions of homology to the genome of said plastid; (f) a gene encoding a selectable marker for selection of a plant cell comprising a plastid expressing said marker; (g) a ribosome binding site joined to said promoter (a) wherein said ribosome binding site is selected from the group consisting of the binding site of the bacteriophage T7 gene 10 leader and the rbcL RBS; and growing a plant cell comprising said transformed plastid under suitable conditions for expression of said protein of interest and said cleavable ubiquitin sequence in said plastid.