Patent ID: 7645571
Filing Date: 2010-01-12
Classification: C12Q,Y10T

Abstract:
1. A method of detecting a target nucleic acid comprising: a) hybridizing a single-stranded or partially single-stranded target nucleic acid to a probe set consisting essentially of an immobilized capture sequence probe and an unlabeled signal sequence probe to form a double stranded hybrid between the target nucleic acid and the capture sequence probe and the signal sequence probe, together forming a hybrid complex, wherein the capture sequence probe and the signal sequence probe hybridize to non-overlapping regions within the target nucleic acid and not to each other, wherein the capture sequence probe comprises an attached ligand, wherein the immobilization of the capture sequence probe and the signal sequence probe occurs through binding of a ligand on the capture sequence probe; and b) adding a blocker probe to the hybridization reaction, wherein said blocker probe hybridizes to excess non-hybridized capture sequence probes; and c) detecting the bound hybrid complex.