Patent ID: 8071312
Filing Date: 2011-12-06
Classification: C12N,C12P,C12Q

Abstract:
1. A method of preparing a nucleic acid molecule, comprising: providing a double stranded nucleic acid molecule; attaching one strand of a stem-loop oligonucleotide comprising an inverted repeat and a loop to the double stranded nucleic acid molecule to produce an oligonucleotide-attached nucleic acid molecule; and i) subjecting the oliognucleotide-attached nucleic acid molecule to strand displacement or nick translation polymerization to generate an endonuclease site or ii) wherein the stem-loop oligonucleotide further comprises a recognition sequence for a nicking endonuclease and wherein the oligonucleotide-attached nucleic acid molecule is amplified by strand displacement synthesis and second strand synthesis.