Patent ID: 7767804
Filing Date: 2010-08-03
Classification: C12N

Abstract:
1. A method for purifying RNA from biological material comprising RNA, comprising the steps of: (a) mixing the biological material with an RNA Lysing/Binding Solution buffered at a pH of less than or equal to 4.5 or greater than or equal to 7, the RNA Lysing/Binding Solution comprising an RNA-complexing salt; (b) contacting the mixture to a solid support such that nucleic acids comprising RNA are in solution in the mixture and preferentially bind to the solid support; (c) washing the solid support with a series of RNA wash solutions to remove biological materials other than bound nucleic acids comprising RNA, wherein the series of wash solutions comprises a first wash comprising alcohol and an RNA-complexing salt at a concentration of at least 1M and a second wash comprising an alcohol and a buffer; and (d) preferentially eluting the bound RNA from the solid support with an RNA Elution Solution in order to obtain substantially pure RNA.