Patent ID: 6479643
Filing Date: 2002-11-12
Classification: A61P,C07K

Abstract:
A biosynthetic single-chain protein comprising:a single polypeptide chain defined by a formula selected from the group consisting of: (a) F1-L-F2-L-H; (b) F2-L-F1-L-H; (c) F2-L-H-L-F1; (d) H-L -F1-L -F2; and (e) H-L-F2-L-F1, wherein F 1 comprises an amino acid sequence comprising amino acid residues 11-22 of SEQ ID NO: 26, wherein Xaa11 is Arg, Asn, Asp, Gln, Glu, His, Lys, Ser or Thr; Xaa13 is Ile, Leu, Met or Val; and Xaa16 is Arg, Asn, Asp, Gln, Glu, His, Lys, Ser or Thr, and H comprises an amino acid sequence comprising amino acid residues 56-63 of SEQ ID NO: 26, wherein Xaa56 is Ala, Arg, Asn, Asp, Cys, Glu, Gln, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr or Val; Xaa57 is Ile, Leu, Met or Val; Xaa58 is Arg, Asn, Asp, Gln, Glu, His, Lys, Ser or Thr; Xaa59 is Ala, Arg, Asn, Asp, Cys, Glu, Gln, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, Val or a peptide bond; Xaa60 is Ala, Arg, Asn, Asp, Cys, Glu, Gln, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, Val or a peptide bond; Xaa61 is Arg, Asn, Asp, Gln, Glu, His, Lys, Ser or Thr; Xaa62 is Ala, Arg, Asn, Asp, Cys, Glu, Gln, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr or Val; and Xaa63 is Ile or Val, and F2 comprises an amino acid sequence comprising amino acid residues 80-89 of SEQ ID NO: 26, wherein Xaa81 is Cys, Ile, Leu, Met, Phe, Trp, Tyr or Val; Xaa83 is Asn, Asp or Glu; Xaa84 is Arg, Asn, Asp, Gln, Glu, His, Lys, Ser or Thr; Xaa85 is Ala, Arg, Asn, Asp, Cys, Glu, Gln, Gly, His, Ile, Leu, Lys, Met, Phe, Pro, Ser, Thr, Trp, Tyr, Val or a peptide bond; Xaa86 is Arg, Asn, Asp, Gln, Glu, His, Lys, Ser or Thr; Xaa87 is Arg, Asn, Asp, Gln, Glu, His , Lys, Ser or Thr; and Xaa89 is Ile or Val, and L comprises a polypeptide linker, and wherein said single-chain protein is capable of inducing endochondral bone formation in a mammal.