Patent ID: 7858340
Filing Date: 2010-12-28
Classification: C12N,C12P

Abstract:
1. A purified polynucleotide molecule comprising a nucleotide sequence encoding a polypeptide, wherein the amino acid sequence of the polypeptide is at least 90% identical to the amino acid sequence of SEQ ID NO:2, said polypeptide comprising substitutions at positions corresponding to amino acid residues selected from the group consisting of: (a) D48E/A89T; (b) S138T/A139T/G179S; (c) Q38P/L136P/E238D; (d) F99S/S138T/A139T/G179S; (e) A139T/M228T; (f) G111V/P289L; and (g) A139T/K154E/Q298H of SEQ ID NO:2; and said polypeptide has the activity of altering the ratio of class 2:1 avermectins to a ratio of about 0.94:1 to about 0.4:1 when the polypeptide is present in