Patent ID: 8361525
Filing Date: 2013-01-29
Classification: A23C,A23L,C12N,C12P

Abstract:
1. A method to increase the quantity of vitamin K2 obtained by cultivating at least one strain of lactic acid bacterium that produces vitamin K2, wherein said strain is cultured in resting-cell conditions, said method comprising at least: a) pre-culturing said strain under respiration conditions, in a suitable pre-culture medium containing at least one porphyrin at a final concentration of at least about 0.5 μg/ml; b) innoculating a suitable culture medium containing at least about 5% fat with a quantity of living bacterial cells of said strain ranging from about 10 c) fermenting the medium thus inoculated for a period ranging from about 4 hours to about 48 hours, at a temperature ranging from about 4° C. to about 50° C., in such a way that, at the end of step c), the quantity of vitamin K2 produced by the resting-cell culture is higher, by a factor at least equal to about 1.2, than that obtained by cultivating said strain under standard fermentation conditions.