Patent ID: 6524792
Filing Date: 2003-02-25
Classification: C12N,C12Q,Y10S

Abstract:
A method for identifying a nucleic acid encoding an exogenous polypeptide which shares a property of interest with members of a class of polypeptides, the nucleic acid being present in a nucleic acid library encoding a plurality of distinct polypeptides, said method comprising:(a) providing a composition comprising a population of eukaryotic host cells, each of which contains an individual expression system, and wherein each individual eukaryotic host cell in substantially all of the population comprises at least one copy of one unique expression system; (b) culturing the population of eukaryotic host cells under conditions where the exogenous polypeptide is expressed; and (c) identifying the nucleic acid by detecting the property of interest, wherein the individual expression systems comprise the individual nucleic acids of the nucleic acid library and wherein said individual expression system directs the generation of infectious viral particles from said eukaryotic host cell.