Patent ID: 6045818
Filing Date: 2000-04-04
Classification: A61K,C07K,C12M,C12N,Y10S

Abstract:
A composition for stimulating the growth of eukaryotic cells comprisinga biocompatible solid substrate,biocompatible synthetic branched water soluble polymeric tethers comprising a polymeric material selected from the group consisting of polyethylene oxide, polyvinyl alcohol, polyhydroxyalkyl (meth)acrylate, polyacrylamide, and starches, andgrowth effector molecules,wherein one end of each tether is covalently linked to the substrate and each growth effector molecule is covalently linked to a distal end of a tether so that the growth effector molecule cannot be internalized by cells attached to the substrate, each tether is able to covalently link more than one growth effector molecule, and the growth effector molecules are attached to the substrate in a concentration effective to enhance the rate of target cell growth over the rate of target cell growth with soluble growth effector molecules and growth effector molecules adsorbed to a substrate without internalization of the molecules; andwherein the substrate is selected from the group consisting of glasses, metals, polystyrenes, polyethylene vinyl acetates, polypropylenes, polymethacrylates, polyacrylates, polyethylenes, polyethylene oxides, polysilicates, polycarbonates, polytetrafluoroethylene, fluorocarbons, nylon, silicon rubber, polyanhydrides, polyglycolic acids, polyhydroxyacids, polyesters, polycapralactone, polyhydroxybutyrate, polyphosphazenes, polyorthoesters, polyurethanes, and combinations thereof.