Patent ID: 7939344
Filing Date: 2011-05-10
Classification: G01N,Y10T

Abstract:
1. A method to identify and recover cells contained within a cell sample that secrete an immunoglobulin of desired specificity and affinity which method comprises: (a) providing onto a sample surface a multiplicity of cell samples, each of which cell samples contains cells that secrete immunoglobulins onto the sample surface, said surface optionally comprising a capture reagent for immunoglobulins, wherein each cell sample is dispensed onto a specified location on the sample surface to which the cells secrete the immunoglobulins; (b) probing said locations on the sample surface with a multiplicity of epitopes and/or antigens each labeled with a distinguishable particulate label wherein said multiplicity includes desired epitopes and/or antigens and undesired epitopes and/or antigens to which the immunoglobulins bind; (c) selecting any location of the surface which comprises immunoglobulins that bind to desired epitopes and/or antigens, but not to undesired epitopes and/or antigens; (d) identifying the cell sample having the cells which secrete immunoglobulins at the selected location; and (e) recovering the identified cell sample.