Patent ID: 7745214
Filing Date: 2010-06-29
Classification: A01K,A61K,C07K,C12N

Abstract:
1. A method for obtaining neural stem cells from a mixed population of cells in a brain tissue sample from an animal, comprising: a) incorporating an FGF1B-detector transgene into a mixed population of brain tissue cells obtained from the animal and allowing the FGF1B-detector transgene to express in said brain tissue cells, wherein the FGF1B detector transgene comprises an FGF1B promoter operably linked to a reporter gene that encodes an assayable product, and wherein the FGF1B detector transgene is incorporated into said brain tissue cells by transfection or electroporation; b) assaying for expression of the assayable product in said brain tissue cells; and c) separating the brain tissue cells that express the assayable product from the brain tissue cells that do not express the assayable product to provide a sub-population of neural stem cells.