Patent ID: 6410697
Filing Date: 2002-06-25
Classification: A61K,C07K

Abstract:
A method for purifying human leukocyte interferon, comprising:(a) treating an antibody that binds at least one species of human leukocyte interferon selected from the group consisting of a species having a molecular weight of 20,180Â±200 daltons, a species having a molecular weight of 19,500Â±200 daltons, a species having a molecular weight of 20,420Â±200 daltons, a species obtained from lymphoblastoid cells and having a molecular weight of 21,130Â±200 daltons and a species obtained from lymphoblastoid cells and having a molecular weight of 23,440Â±200 daltons, molecular weights as determined under non-reducing SDS PAGE, to remove proteolytic activity; (b) applying a solution of human leukocyte interferon to a matrix having immobilized thereon the treated antibody of (a); and (c) eluting said human leukocyte interferon from the matrix.