Patent ID: 8420345
Filing Date: 2013-04-16
Classification: C12Q

Abstract:
1. A method for identifying two groups of microorganisms expressing a same enzymatic activity, comprising the following steps: a) incubating the two groups of microorganisms in a reaction medium comprising a first enzyme substrate and a second enzyme substrate, said first and second enzyme substrates being capable of being metabolized by the same enzymatic activity selected from the group consisting of β-D-glucosidase, β-D-galactosidase, alpha-D-glucosidase, alpha-D-galactosidase, alpha-mannosidase, β-D-glucuronidase, N-acetyl-β-D-hexosaminidase, β-D-cellobiosidase, esterase, phosphatase, phospholipase, sulfatase, and peptidase; and b) identifying the two groups of microorganisms based on differences between signals produced by the two groups of microorganisms from hydrolysis of the first and second enzyme substrates; wherein: