Patent ID: 7307150
Filing Date: 2007-12-11
Classification: A61K,A61P,C08B

Abstract:
1. A method for preparing an oxygen carrying compound, said compound comprising a conjugate of hemoglobin covalently bound to a dextran molecule, said method comprising: 1) reacting the dextran with a bromine compound to provide bromine containing groups on said dextran, thereby providing an activated dextran; 2) filtering of said activated dextran with a first filter having a pore size that results in a retentate having a molecular weight greater than 500 kD; 3) reacting the activated dextran filtrate of step (2) with hempglobin therby providing a conjugated dextran-hemoglobin molecule; 4) filtering said dextran-hemoglobin molecule with a filter having a pore size that reults in a retentate having a molecular weight greaterthan 500 kD, and further filtering through a filter having a pore size that results in a filtrate containing molecules having a weight greater than 80 kD.