Patent ID: 6727411
Filing Date: 2004-04-27
Classification: C12N

Abstract:
A method of producing a transgenic tomato containing, in a fruit of the transgenic tomato, one or more free amino acids in an amount larger than that contained in the same natural plants cultured under the same conditions, which comprises the steps ofa) transforming a tomato with a genetic construct containing the antisense sequence of a gene encoding glutamate decarboxylase (GAD), a regulatory sequence capable of expressing the antisense sequence and a marker gene, wherein said gene encoding GAD has the sequence having at least 85% homology to a sequence of SEQ ID NO: 1; b) selecting or identifying the transgenic tomato based on a character imparted by the marker gene in the genetic construct; c) screening the transgenic tomato in which one or more free amino acids are accumulated in a larger amount; and d) selecting the transgenic tomato in which one or more free amino acids are accumulated in a larger amount.