Patent ID: 6806053
Filing Date: 2004-10-19
Classification: B01L,C07K,C12Q,G01N

Abstract:
A method of identifying a compound as a modulator of G-protein-linked receptor activity that generates or consumes a cyclic nucleotide through the action of a cyclase or phosphodiesterase, comprising:conducting a G-protein-linked-receptor mediated reaction that generates or consumes a cyclic nucleotide in the presence of a candidate compound; contacting, in vitro, a product of the reaction with a luminescent tracer and with the opposite member of a specific binding pair to the cyclic nucleotide, wherein the tracer and the cyclic nucleotide compete for binding to the opposite member of the specific binding pair; illuminating the tracer with polarized light, wherein the light is capable of inducing emission of polarized light from the tracer; detecting the extent of polarization of light emitted from the tracer; and identifying the candidate compound as a modulator of the reaction based on the extent of polarization of the emitted light.