Patent ID: 6777215
Filing Date: 2004-08-17
Classification: C12N,C12P,C12Y

Abstract:
A cyclomaltodextrin glucanotransferase variant having an amino acid sequence which differs from the amino acid sequence of a parent cyclomaltodextrin glucanotransferase, wherein the parent cyclomaltodextrin glucanotraneferase is a Bacillus cyclomaltodextrin glucanotransferase and the difference between the amino acid sequence of the cyclomaltodextrin glucenotransferase variant and the amino acid sequence of the parent cyclomaltodextrin glucanotransferase comprises one or more of the following:47C; 47D; 47TE; 47F; 47G; 47I; 47K; 47N; 47P; 47S; 47T; 47V; 47W; 47Y; 145D; 145H; 145I; 145N; 145Q; 145V; 146H, 146L; 146T; 146V; 146Y; 147C; 147E; 147N; 147Q; 196C; 196E; 196F; 196H; 196I; 196K; 196M; 196P; 196Q; 196R; 196T; 196V; 196W; 196Y; and 371C; 371F; 371H; 371K; 371M; 371R; 371T; 371W; wherein each position corresponds to the position of the amino acid sequence of the mature Cyclomaltodextrin glucanotransferase obtained from Bacillus circulans strain 251.