Patent ID: 8748174
Filing Date: 2014-06-10
Classification: A61P,C12N

Abstract:
1. A process for the production of influenza viruses comprising: a) infecting adherent and non-tumorigenic Madin Darby Canine Kidney (MDCK) cells with influenza viruses, which cells are adapted to grow and cultivated in serum-free medium comprising Taub's medium, lipids and wheat hydrolysate, which Taub's medium comprises a 50:50 mixture of DMEM and Ham's F12, 4.5 grams per liter glucose, 4 millimolar L-glutamine, 5 micrograms per milliliter insulin, 5 micrograms per milliliter transferrin, 5 picomolar triiodothyronine, 50 nanomolar hydrocortisone, 25 nanograms per milliliter prostaglandin E1 and 10 nanomolar sodium selenite; b) incubating the cells; and c) isolating the influenza viruses from the cells.