Patent ID: 8221701
Filing Date: 2012-07-17
Classification: B01L,G01N,Y10T

Abstract:
1. A microfluidic device comprising: a rotatable platform; a specimen chamber that is disposed in the platform and accommodates a specimen; a specimen distribution unit that is disposed to be connected to the specimen chamber, wherein the specimen is centrifugally separated into a supernatant and a sediment, and wherein the specimen distribution unit distributes a predetermined amount of separated supernatant through specimen distribution channels; a plurality of dilution chambers that are respectively disposed to be connected to the respective specimen distribution channels and serve as a container for a dilution buffer, wherein the specimen flows from the specimen distribution channels into the respective dilution chambers, and wherein the specimen is combined with the dilution buffer in the respective dilution chambers to provide diluted specimen solutions, each having a dilution ratio different from all or some of other diluted specimen solutions; and a plurality of reaction chamber unit groups which each is connected to the respective dilution chambers, each of the plurality of reaction chamber unit groups comprising at least one reaction chamber unit, and wherein each reaction chamber unit group serves as a container for a reagent and accommodate the diluted specimen solution distributed from the corresponding dilution chamber of the plurality of dilution chambers through a plurality of diluted specimen distribution channels due to the rotation of the platform, wherein the reagent and the diluted specimen solution are combined in the reaction chamber, wherein the plurality of dilution chambers comprises a first chamber having a first volume and a second chamber having a second volume, said second volume being less than the first volume.