Patent ID: 7211409
Filing Date: 2007-05-01
Classification: C07C,C07K,G01N

Abstract:
1. An immunoassay method for determining the concentration of dioxins in a sample, the method comprising the following steps: 1) allowing target dioxins in the sample and a competitive antigen to competitively react with a primary anti-dioxin antibody capable of binding to the target dioxins, and determining the amount of competitive antigen-antibody complex from a label incorporated into a secondary antibody binding to the primary antibody; 2) allowing the competitive antigen and a compound of formula (1) of known concentration wherein R 1 , R 2 , R 3 and R 4 may be the same or different and each represents chlorine or hydrogen, n is an integer from 1 to 10, and Z represents 1 to 100 amino acid residues to competitively react with the primary anti-dioxin antibody, and determining the amount of competitive antigen-antibody complex from a label incorporated into a secondary antibody binding to the primary antibody; 3) preparing a calibration curve using the amount of competitive antigen-antibody complex determined in step 2); and 4) comparing the amount of competitive antigen-antibody complex determined in step 1) with the calibration curve prepared in step 3).