Patent ID: 9128090
Filing Date: 2015-09-08
Classification: C07K,G01N

Abstract:
1. The method for selecting eukaryotic clones highly expressing a polypeptide of interest from a population of eukaryotic cells expressing said polypeptide of interest comprising: (a) culturing said population of eukaryotic cells on a semi-solid culture medium; (b) contacting said population of cultured eukaryotic cells with a capture molecule that interacts with said polypeptide of interest, wherein the concentration of said capture molecule is between about 0.2 and 2.0 mg/ml, and said capture molecule comprises a detectable label; (c) incubating said population of cultured eukaryotic cells expressing said polypeptide of interest with said capture molecule for a period of time; (d) measuring the relative size of haloes produced by said detectable label following interaction of said polypeptide of interest with said capture molecule and wherein high expressing eukaryotic clones have a halo with a width that is equal to, or greater than, the radius of a eukaryotic cell clone lacking a halo; and (e) selecting at least one of said high expressing eukaryotic cell clones from said population of said eukaryotic cells.