Patent ID: 8999652
Filing Date: 2015-04-07
Classification: G01N,Y10T

Abstract:
1. A method of distinguishing between cell types in a sample comprising a plurality of different cell types comprising: a) labeling at least two different signal markers expressed by at least one cell type in the sample with distinguishably labeled binding proteins, wherein the at least two different signal markers are exogenous cell surface proteins, wherein said exogenous cell surface proteins are constitutively expressed from one or more genetic constructs, and wherein at least one of said signal markers is expressed at least at three different amounts in said plurality of different cell types; b) detecting from at least one cell type of said plurality of different cell types the at least two different, distinguishably labeled signal markers; c) measuring at least one of said labeled signal markers of step b) at three different intensities corresponding to said at least three different amounts in the plurality of different cell types, wherein the combination of the intensity and type of signal markers in a single cell type provides a unique barcode; and d) distinguishing at least one cell type of said plurality of different cell types based on the unique barcode provided in c).