Patent ID: 8518686
Filing Date: 2013-08-27
Classification: C07K,C12N,C12P,C12Y

Abstract:
1. An isolated host cell comprising an isoprene synthase variant produced by: a) identifying hydrophobic amino acid residues on the surface of isoprene synthase based on the three dimensional structure of b) introducing one or more amino acid substitutions in the surface amino acids of said isoprene synthase to produce an isoprene synthase variant; and c) identifying said isoprene synthase variants with substitutions that improve activity, expression or stability of the isoprene synthase, wherein said isoprene synthase variant comprises one or more amino acid substitutions at a hydrophobic surface amino acid position corresponding to a hydrophobic surface amino acid position of SEQ ID NO: 45 selected from the group I28, V30, L130, G153, L469, L303 and L494; and wherein said isoprene synthase variant has improved activity, expression or stability of the isoprene synthase compared to an isoprene synthase without said one or more amino acid substitutions.