Patent ID: 7463791
Filing Date: 2008-12-09
Classification: G06K,H01J

Abstract:
1. A method for automatic alignment of tilt series in an electron microscope, comprising: applying markers to a sample to be imaged by the electron microscope; providing a tilt series of images of the sample; identifying a first set of candidate markers in each of the images in the tilt series; attributing at least one probability parameter to each candidate marker in each image; characterized in that the method further comprises: selecting a second set as a subset of candidate markers from the first set of candidate markers on the basis of said at least one probability parameter; projecting the candidate markers in the second set onto a sole image; applying a fitting algorithm to determine a set of parallel straight lines or very elongate ellipses best fitting the candidate markers in the sole image to identify a third subset of candidate markers; aligning the images in the tilt series on the basis of the third subset of identified candidate markers.