Patent ID: 6207812
Filing Date: 2001-03-27
Classification: C07K,G01N

Abstract:
# SEQUENCE LISTING <160> NUMBER OF SEQ ID NOS: 8 <210> SEQ ID NO 1 <211> LENGTH: 164 <212> TYPE: DNA <213> ORGANISM: Homo sapiens <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(156) <400> SEQUENCE: 1 atg gct gcg ggt ccc agg cca gga gct ccc tg #c agg gcg ggg gct ccc 48 Met Ala Ala Gly Pro Arg Pro Gly Ala Pro Cy #s Arg Ala Gly Ala Pro 1 5 # 10 # 15 acg atc gta ttg acc tct gga aga aga cag ac #a ctt tcc cac ggg agc 96 Thr Ile Val Leu Thr Ser Gly Arg Arg Gln Th #r Leu Ser His Gly Ser 20 # 25 # 30 tcc tct cca gcc aga gct aca ctt ggc aaa cc #t ttg gtc cta aat gat 144 Ser Ser Pro Ala Arg Ala Thr Leu Gly Lys Pr #o Leu Val Leu Asn Asp 35 # 40 # 45 tat tca ctg aat tgaagaaa # # #164 Tyr Ser Leu Asn 50 <210> SEQ ID NO 2 <211> LENGTH: 52 <212> TYPE: PRT <213> ORGANISM: Homo sapiens <400> SEQUENCE: 2 Met Ala Ala Gly Pro Arg Pro Gly Ala Pro Cy #s Arg Ala Gly Ala Pro 1 5 # 10 # 15 Thr Ile Val Leu Thr Ser Gly Arg Arg Gln Th #r Leu Ser His Gly Ser 20 # 25 # 30 Ser Ser Pro Ala Arg Ala Thr Leu Gly Lys Pr #o Leu Val Leu Asn Asp 35 # 40 # 45 Tyr Ser Leu Asn 50 <210> SEQ ID NO 3 <211> LENGTH: 884 <212> TYPE: DNA <213> ORGANISM: Homo sapiens <400> SEQUENCE: 3 acttccctgg gttcacagca ggggtggaac tggattcttc ctggatgggg at #ccagatgg 60 aggtggagct gcaccccttg tagagaatgg ctgcgggtcc caggccagga gc #tccctgca 120 gggcgggggc tcccacgatc gtattgacct ctggaagaag acagacactt tc #ccacggga 180 gctcctctcc agccagagct acacttggca aacctttggt cctaaatgat ta #ttcactga 240 attgaagaaa tacggtttac atatcttcca agtatatatg tagggttgat tt #gggaagca 300 gaacacagca gcccaaattt gcttgtaatg tctgcgacta cagcctgctg gc #ctgccttc 360 actgtcttgg gggaagctcg gggagaccag gtggactgga gtagactgtg ca #gagacact 420 ggtctggtga agatgtccag gaaaccacga gcctccagcc cattttccaa ca #accaccca 480 tcaacaccaa agaggttccc aagacaaccc agaagggaaa agggacccgt ca #aggaagtt 540 ccaggaacaa aaggctctcc ctaaaagacc accgcttcaa aaaaacctga gg #aatggagt 600 gggccaacac tatccagcca ctctgaccag ccgaacgagg aactcaatca aa #atgcgcca 660 tagcaggacc acaagggcaa ggagaccacc gccttctcca gtgcttcctt gg #gcagccag 720 taattcccag gcaaggccag agacttcaag tctatctgaa aagtctccag aa #gtctaacc 780 ccagataaat agccaacagg gtgtagagta cgttttacac ccaaagggta at #gccccatg 840 gtgatggaaa taaaatgaac atgttgtaaa atgaaaaaaa aaaa # #884 <210> SEQ ID NO 4 <211> LENGTH: 14 <212> TYPE: DNA <213> ORGANISM: Artificial Sequence <220> FEATURE: <223> OTHER INFORMATION: synthetically generated <220> FEATURE: <221> NAME/KEY: miscfeature <222> LOCATION: (1)...(14) <223> OTHER INFORMATION: n = A,T,C or G <400> SEQUENCE: 4 tttttttttt ttvn # # # 14 <210> SEQ ID NO 5 <211> LENGTH: 1946 <212> TYPE: DNA <213> ORGANISM: Homo sapiens <220> FEATURE: <221> NAME/KEY: miscfeature <222> LOCATION: (1)...(1946) <223> OTHER INFORMATION: n = A,T,C or G <400> SEQUENCE: 5 cacgcaaagc agtgtgggtt gattctgagg tgcactgtgg gaaagagctt gt #cgctgcgg 60 tgttgctgtt ggagactcga ttgttggtga cagcgaaaga acgataacaa aa #tgccggag 120 cgagatagtg agccgttctc caaccctttg gcccccgatg gccacgatgt gg #atgatcct 180 cactccttcc accaatcaaa actcaccaat gaagacttca ggaaantnnt ca #tgaccccc 240 agggntgcac ntacntntgc accacnttnt aantnnnntc accatgagat gc #caagggag 300 tacaatgagg atgaagaccc agctgcacga aggaggaaaa agaaaagtta tt #atgccaag 360 ctacgccaac aagaaattga gagagagaga gagctagcag agaagtaccg gg #atcgtgcc 420 aaggaacgga gagatggagt gaacaaagat tatgaagaaa ccgagcttat ca #gcaccaca 480 gctaactata gggctgttgg ccccactgct gaggcggaca aatcagctrc ag #nnragaga 540 agacanwnda hcnaggagtc caaattcttg ggtggtgaca tggaacacac cc #atttggtg 600 aaaggcttgg attttgntnt gcttchnaan gtncgagctg agattgncms cm #nanaraaa 660 nargaarang nnctgatggn aaanccccmg aaagaaacca agaaagatga gg #atcctgaa 720 aataaaattg aatttaaaac acgtctgggc cgcaatgttt accgaatgct tt #ttaagagc 780 aaagcatatg agcggaatga gttgttcctg ccgggccgca tggcctatgt gg #tagacctg 840 gatgatgagt atgctgacac agatatcccc accactctta tcccgcagca ag #gctgattg 900 ccccaccatg gaggcccaga ccacactgac cacaaatgac attgtcatta gc #aagctgac 960 ccagatcctt tcatacctga ggcagggaac ccgtaacaag aagcttaaga ag #aaggataa 1020 agggaagccg gaagagaaga aacctcctga ggctgacatg aatatttttg aa #gacattgg 1080 ggattacgta ccctccacaa ccaagacacc tcgggacaag gagcgggaga ga #tatcggga 1140 acgggagcgt gatcgggaaa gagacagaga ccgtgaccga gagcgagagc ga #gaacgaga 1200 tcgggaacga gagcgagagc gggaccgaga gagagaagag gaaaagaaga ga #cacagcta 1260 ctttgagaag ccaaaagtag atgatgagcc catggacgtt gacaaaggac ct #gggtctac 1320 caaggagttg atcaagtcca tcaatgaaaa gtttgctggg tctgctggct gg #gaaggcac 1380 agaatcgctg aagaagccag aagacaaaaa gcagctggga gatttctttg gc #atgtccaa 1440 cagttatgca gagtgctacc cagccacgat ggatgacatg gctgtggata gt #gatgagga 1500 ggtggattat agcaaaatgg accagggtaa caagaagggg cccttaggcc gt #tgggactt 1560 tgatacccag gaagaataca gcgagtatat gaacaacaaa gaagctttgc cc #aaggctgc 1620 attccagtat ggtatcaaaa tgtctgaagg gcggaaaacc aggcgcttca ag #gaaaccaa 1680 tgacaaagca gagcttgatc gccagtggaa gaagattagt gcaatcattg an #gaagagga 1740 agaagatgga agctgatggg gttgaagtca aaagaccaaa atactaatca ct #agttacaa 1800 ccagagatgc tccacaagga tatgctcccc actgttttct ttctacaatt tc #caaaggtt 1860 gcaagatgtt tttttgtgat gaatataaaa ttttattgtg taattacttg gt #tccattaa 1920 aattggttaa cttgctaaaa aaaaaa # # 1946 <210> SEQ ID NO 6 <211> LENGTH: 915 <212> TYPE: DNA <213> ORGANISM: Homo sapiens <220> FEATURE: <221> NAME/KEY: CDS <222> LOCATION: (1)...(912) <221> NAME/KEY: miscfeature <222> LOCATION: (1)...(915) <223> OTHER INFORMATION: n = A,T,C or G <400> SEQUENCE: 6 atg atg agt atg ctg aca cag ata tcc cca cc #a ctc tta tcc cgc agc 48 Met Met Ser Met Leu Thr Gln Ile Ser Pro Pr #o Leu Leu Ser Arg Ser 1 5 # 10 # 15 aag gct gat tgc ccc acc atg gag gcc cag ac #c aca ctg acc aca aat 96 Lys Ala Asp Cys Pro Thr Met Glu Ala Gln Th #r Thr Leu Thr Thr Asn 20 # 25 # 30 gac att gtc att agc aag ctg acc cag atc ct #t tca tac ctg agg cag 144 Asp Ile Val Ile Ser Lys Leu Thr Gln Ile Le #u Ser Tyr Leu Arg Gln 35 # 40 # 45 gga acc cgt aac aag aag ctt aag aag aag ga #t aaa ggg aag ccg gaa 192 Gly Thr Arg Asn Lys Lys Leu Lys Lys Lys As #p Lys Gly Lys Pro Glu 50 # 55 # 60 gag aag aaa cct cct gag gct gac atg aat at #t ttt gaa gac att ggg 240 Glu Lys Lys Pro Pro Glu Ala Asp Met Asn Il #e Phe Glu Asp Ile Gly 65 # 70 # 75 # 80 gat tac gta ccc tcc aca acc aag aca cct cg #g gac aag gag cgg gag 288 Asp Tyr Val Pro Ser Thr Thr Lys Thr Pro Ar #g Asp Lys Glu Arg Glu 85 # 90 # 95 aga tat cgg gaa cgg gag cgt gat cgg gaa ag #a gac aga gac cgt gac 336 Arg Tyr Arg Glu Arg Glu Arg Asp Arg Glu Ar #g Asp Arg Asp Arg Asp 100 # 105 # 110 cga gag cga gag cga gaa cga gat cgg gaa cg #a gag cga gag cgg gac 384 Arg Glu Arg Glu Arg Glu Arg Asp Arg Glu Ar #g Glu Arg Glu Arg Asp 115 # 120 # 125 cga gag aga gaa gag gaa aag aag aga cac ag #c tac ttt gag aag cca 432 Arg Glu Arg Glu Glu Glu Lys Lys Arg His Se #r Tyr Phe Glu Lys Pro 130 # 135 # 140 aaa gta gat gat gag ccc atg gac gtt gac aa #a gga cct ggg tct acc 480 Lys Val Asp Asp Glu Pro Met Asp Val Asp Ly #s Gly Pro Gly Ser Thr 145 1 #50 1 #55 1 #60 aag gag ttg atc aag tcc atc aat gaa aag tt #t gct ggg tct gct ggc 528 Lys Glu Leu Ile Lys Ser Ile Asn Glu Lys Ph #e Ala Gly Ser Ala Gly 165 # 170 # 175 tgg gaa ggc aca gaa tcg ctg aag aag cca ga #a gac aaa aag cag ctg 576 Trp Glu Gly Thr Glu Ser Leu Lys Lys Pro Gl #u Asp Lys Lys Gln Leu 180 # 185 # 190 gga gat ttc ttt ggc atg tcc aac agt tat gc #a gag tgc tac cca gcc 624 Gly Asp Phe Phe Gly Met Ser Asn Ser Tyr Al #a Glu Cys Tyr Pro Ala 195 # 200 # 205 acg atg gat gac atg gct gtg gat agt gat ga #g gag gtg gat tat agc 672 Thr Met Asp Asp Met Ala Val Asp Ser Asp Gl #u Glu Val Asp Tyr Ser 210 # 215 # 220 aaa atg gac cag ggt aac aag aag ggg ccc tt #a ggc cgt tgg gac ttt 720 Lys Met Asp Gln Gly Asn Lys Lys Gly Pro Le #u Gly Arg Trp Asp Phe 225 2 #30 2 #35 2 #40 gat acc cag gaa gaa tac agc gag tat atg aa #c aac aaa gaa gct ttg 768 Asp Thr Gln Glu Glu Tyr Ser Glu Tyr Met As #n Asn Lys Glu Ala Leu 245 # 250 # 255 ccc aag gct gca ttc cag tat ggt atc aaa at #g tct gaa ggg cgg aaa 816 Pro Lys Ala Ala Phe Gln Tyr Gly Ile Lys Me #t Ser Glu Gly Arg Lys 260 # 265 # 270 acc agg cgc ttc aag gaa acc aat gac aaa gc #a gag ctt gat cgc cag 864 Thr Arg Arg Phe Lys Glu Thr Asn Asp Lys Al #a Glu Leu Asp Arg Gln 275 # 280 # 285 tgg aag aag att agt gca atc att gan gaa ga #g gaa gaa gat gga agc 912 Trp Lys Lys Ile Ser Ala Ile Ile Xaa Glu Gl #u Glu Glu Asp Gly Ser 290 # 295 # 300 tga # # # 915 <210> SEQ ID NO 7 <211> LENGTH: 304 <212> TYPE: PRT <213> ORGANISM: Homo sapiens <220> FEATURE: <221> NAME/KEY: VARIANT <222> LOCATION: (1)...(304) <223> OTHER INFORMATION: Xaa = Any Amino Aci #d <400> SEQUENCE: 7 Met Met Ser Met Leu Thr Gln Ile Ser Pro Pr #o Leu Leu Ser Arg Ser 1 5 # 10 # 15 Lys Ala Asp Cys Pro Thr Met Glu Ala Gln Th #r Thr Leu Thr Thr Asn 20 # 25 # 30 Asp Ile Val Ile Ser Lys Leu Thr Gln Ile Le #u Ser Tyr Leu Arg Gln 35 # 40 # 45 Gly Thr Arg Asn Lys Lys Leu Lys Lys Lys As #p Lys Gly Lys Pro Glu 50 # 55 # 60 Glu Lys Lys Pro Pro Glu Ala Asp Met Asn Il #e Phe Glu Asp Ile Gly 65 # 70 # 75 # 80 Asp Tyr Val Pro Ser Thr Thr Lys Thr Pro Ar #g Asp Lys Glu Arg Glu 85 # 90 # 95 Arg Tyr Arg Glu Arg Glu Arg Asp Arg Glu Ar #g Asp Arg Asp Arg Asp 100 # 105 # 110 Arg Glu Arg Glu Arg Glu Arg Asp Arg Glu Ar #g Glu Arg Glu Arg Asp 115 # 120 # 125 Arg Glu Arg Glu Glu Glu Lys Lys Arg His Se #r Tyr Phe Glu Lys Pro 130 # 135 # 140 Lys Val Asp Asp Glu Pro Met Asp Val Asp Ly #s Gly Pro Gly Ser Thr 145 1 #50 1 #55 1 #60 Lys Glu Leu Ile Lys Ser Ile Asn Glu Lys Ph #e Ala Gly Ser Ala Gly 165 # 170 # 175 Trp Glu Gly Thr Glu Ser Leu Lys Lys Pro Gl #u Asp Lys Lys Gln Leu 180 # 185 # 190 Gly Asp Phe Phe Gly Met Ser Asn Ser Tyr Al #a Glu Cys Tyr Pro Ala 195 # 200 # 205 Thr Met Asp Asp Met Ala Val Asp Ser Asp Gl #u Glu Val Asp Tyr Ser 210 # 215 # 220 Lys Met Asp Gln Gly Asn Lys Lys Gly Pro Le #u Gly Arg Trp Asp Phe 225 2 #30 2 #35 2 #40 Asp Thr Gln Glu Glu Tyr Ser Glu Tyr Met As #n Asn Lys Glu Ala Leu 245 # 250 # 255 Pro Lys Ala Ala Phe Gln Tyr Gly Ile Lys Me #t Ser Glu Gly Arg Lys 260 # 265 # 270 Thr Arg Arg Phe Lys Glu Thr Asn Asp Lys Al #a Glu Leu Asp Arg Gln 275 # 280 # 285 Trp Lys Lys Ile Ser Ala Ile Ile Xaa Glu Gl #u Glu Glu Asp Gly Ser 290 # 295 # 300 <210> SEQ ID NO 8 <211> LENGTH: 16 <212> TYPE: PRT <213> ORGANISM: Homo sapiens <400> SEQUENCE: 8 Arg Arg Gln Thr Leu Ser His Gly Ser Ser Se #r Pro Ala Arg Ala Cys 1 5 # 10 # 151. An isolated nucleic acid molecule encoding a chondrosarcoma associated (CSA) polypeptide, wherein said nucleic acid molecule comprises the nucleotide sequence of SEQ ID NO:1.