Patent ID: 6613526
Filing Date: 2003-09-02
Classification: A61K,C12N,C12Q

Abstract:
A method for identifying nucleic ligands to target molecules within a blood brain barrier or CSF-blood barrier tissue comprising:a) preparing a candidate mixture of nucleic acid sequences; b) contacting said candidate mixture of nucleic acids with said blood brain barrier or CSF-blood barrier tissue, wherein nucleic acids having an increased affinity to the target molecules relative to the candidate mixture may be partitioned from the remainder of the candidate mixture; c) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture; and d) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acid sequences with relatively higher affinity and specificity for binding to said target molecules, whereby nucleic acid ligands of said target molecules may be identified.