Patent ID: 7238522
Filing Date: 2007-07-03
Classification: B01F,B03D,C12M,C12N

Abstract:
1. An apparatus for isolating cellular component of interest from cells comprising: (a) a first tank, wherein the first tank is used for holding a suspension of cells having the cellular component of interest; (b) a mixer in fluid communication with the first tank; (c) a second tank in fluid communication with the mixer, wherein the second tank is used for holding a lysis solution; (d) a holding coil in fluid communication with the mixer; and (e) a bubble-mixer chamber with a top and a bottom having: wherein, the mixer is a high-shear, low-residence-time-mixing-device, wherein the residence-time is less than or equal to about one second; the suspension of cells having the plasmid DNA from the first tank is allowed to flow into the mixer; the lysis solution from the second tank is allowed to flow into the mixer; a lysate-mixture is allowed to flow from the mixer into the holding coil; the lysate-mixture from the holding coil is allowed to flow into the bubble-mixer chamber; the precipitation solution, the neutralization solution, or the mixture thereof from the third tank is allowed to flow into the bubble-mixer chamber; and a suspension containing the cellular component of interest is allowed to flow from the bubble-mixer chamber into the fourth tank.