Patent ID: 7939468
Filing Date: 2011-05-10
Classification: A61P,C12N,C12Q

Abstract:
1. A method for identifying a compound that modulates the ability of animalia tRNA splicing endonuclease to produce mature tRNA, the method comprising: (a) contacting a compound or a compound from a library of compounds with an animalia cell that contains the animalia tRNA splicing endonuclease and expresses a full-length protein encoded by the coding region of a reporter gene of a nucleic acid substrate, wherein the nucleic acid substrate comprises the coding region of a reporter gene and a tRNA intron in a mature domain of a precursor tRNA, and wherein the tRNA intron is contained within the nucleic acid substrate such that the transcribed mRNA from the coding region of the reporter gene is out of frame; and (b) detecting the amount of full-length protein expressed, wherein an alteration in the amount of the full-length protein expressed in the presence of the compound or the compound from the library of compounds relative to the amount of the full-length protein expressed in the absence of the compound or the compound from a library of compounds, or in the presence of a negative control indicates that the compound or the compound from the library of compounds modulates the ability of animalia tRNA splicing endonuclease to produce mature tRNA.