Patent ID: 9180143
Filing Date: 2015-11-10
Classification: A61F,A61K,A61L,A61P,C12N,G01N

Abstract:
1. A method of implanting a tissue matrix in bladder reconstruction or replacement surgery comprising: immersing a distensible membranous sac in a buffer solution at a mild alkaline pH which includes active amounts of a proteolytic inhibitor; (ii) distending the distensible membranous sac by introducing a sufficient volume of the same buffer solution into the interior cavity of the sac; (iii) continuing decellularisation of the sac by replacing and introducing fresh solutions around the exterior surface of the sac and/or into the sac interior so as to maintain distension of the sac during recellularisation; and (iv) implanting the whole decellularised distensible sac or a portion thereof into a donor.