Patent ID: 8628969
Filing Date: 2014-01-14
Classification: G01N,Y10T

Abstract:
1. A method for quantitative analysis of β-cryptoxanthin contained in a sample, comprising: mixing the sample with a diethyl ether solution containing an internal standard substance selected from the group consisting of lycopene and retinal, wherein the sample is a liquid containing fruit juice or vegetable juice; obtaining a diethyl ether layer; adding a dehydrating agent to the obtained diethyl ether layer to dehydrate the diethyl ether layer; after the dehydration, adding an alkali solution to the dehydrated diethyl ether layer to conduct saponification reaction; after the reaction, adding an acid, allowing the mixture to stand still, and then obtaining a diethyl ether layer; and subjecting the diethyl ether layer to high-performance liquid chromatography and conducting quantitative analysis of β-cryptoxanthin using an internal standard method.