Patent ID: 6218517
Filing Date: 2001-04-17
Classification: A61K,C12N,C12Y

Abstract:
A method for producing angiostatin, which is derived from human plasminogen and has sequence on amino acids 355-791 of human plasminogen, said method comprising the steps of:separating human plasminogen into Form 1 and Form 2 thereof by a first Lysine-Sepharose chromatography;separately hydrolyzing each of Form 1 and Form 2 of plasminogen with an elastase;fractionating, by a second Lysine-Sepharose chromatography, a hydrolyzate resulting from the above hydrolyzing step of the Form 1 of plasminogen;collecting fractions unbound on a Lysine-Sepharose column used in the second Lysine-Sepharose chromatography;further fractionating, by a first Aminohexal-Sepharose chromatography, the fractions collected as a result of the second Lysine-Sepharose chromatography;collecting fractions unbound on an Aminohexal-Sepharose column used in the first Aminohexal-Sepharose chromatography;fractionating, by a third Lysine-Sepharose chromatography, a hydrolyzate resulting from the above hydrolyzing step of the Form 2;collecting fractions bound on a Lysine-Sepharose column used in the third Lysine-Sepharose chromatography;fractionating, by a second Aminohexal-Sepharose chromatography, the fractions collected as a result of the third Lysine-Sepharose chromatography; andcollecting fractions unbound on an Aminohexal-Sepharose column of the second Aminohexal-Sepharose chromatography.