Patent ID: 8586317
Filing Date: 2013-11-19
Classification: A01K,A61K,A61P,C07K,C12Q

Abstract:
1. A method of diagnosing a hypophosphatemic disorder in a mammal, said method comprising (a) obtaining a biological sample from said mammal and (b) contacting said biological sample with a reagent that detects the level of fibroblast growth factor-23 (FGF23) polypeptide in said sample, wherein the FGF23 polypeptide is a polypeptide selected from the group consisting of a polypeptide comprising the amino acid sequence of SEQ ID NO:2, a polypeptide comprising the amino acid sequence of SEQ ID NO:4, a polypeptide comprising an arginine-to-glutamine mutation at amino acid 176 (R176Q) relative to SEQ ID NO:2, a polypeptide comprising an arginine-to-glutamine mutation at amino acid 179 (R179Q) relative to SEQ ID NO:2, and a polypeptide comprising an arginine-to-tryptophan mutation at amino acid 179 (R179W) relative to SEQ ID NO:2, wherein an elevated level of FGF23 polypeptide in said sample, relative to the level of FGF23 polypeptide in a sample obtained from a control mammal, is an indication that said mammal is afflicted with said hypophosphatemic disorder, thereby diagnosing said hypophosphatemic disorder in said mammal.