Patent ID: 7045308
Filing Date: 2006-05-16
Classification: G01N

Abstract:
1. A method of screening for an immunosuppressant, comprising: a) combining a USP-25 protein, a USP-25 target protein which is conjugated to ubiquitin, and a candidate bioactive agent, wherein said USP-25 protein has ubiquitin-specific peptidase activity and wherein said USP-25 protein comprises an amino acid sequence having at least about 95% identity to the amino acid sequence set forth in SEQ ID NO:2; and b) determining the level of ubiquitin-conjugated target protein in the presence of said candidate bioactive agent; wherein an increase in the level of ubiquitin-conjugated target protein in the presence of said candidate bioactive agent indicates that said candidate bioactive agent is an immunosupressant.