Patent ID: 6335173
Filing Date: 2002-01-01
Classification: G01N,Y10S

Abstract:
A method of detecting the presence of an analyte on a surface of one or more cells by flow cytometry, the method comprising:a) specifically coating one or more unfixed cells comprising a surface analyte with tyramide by catalyzing the deposition of tyramide on the surface of said cells, wherein said coating of one or more cells is performed under conditions that inhibit bystander staining; b) contacting said one or more cells coated with tyramide with a detectable label that directly or indirectly binds to tyramide; and c) detecting a signal from said label using a flow cytometric device that is at least 10-fold greater than a signal obtainable by standard flow cytometry methods, wherein said signal indicates the presence of said analyte on the surface of said cells.