Patent ID: 8835132
Filing Date: 2014-09-16
Classification: C07K

Abstract:
1. A method for making an acylated glucagon-like peptide-1 (GLP-1), wherein said GLP-1 comprises at least one lysine residue that is acylated on its ε-amino group, and wherein said GLP-1 comprises GLP-1 (7-37) acylated at position Lys 26 , said method comprising: (i) expressing in a yeast cell a GLP-1 precursor of said GLP-1, wherein said GLP-1 precursor comprises said GLP-1 and an N-terminal extension, wherein the N-terminal extension is up to 15 amino acids in length, said N-terminal extension being cleavable from the GLP-1 at a lysine cleavage site; (ii) acylating the ε-amino group of said at least one lysine residue in the GLP-1 without acylating the ε-amino group of the lysine cleavage site, to produce an acylated GLP-1 precursor; and (iii) removing the N-terminal extension from the acylated GLP-1 precursor by enzymatic cleavage to produce said acylated GLP-1, wherein said N-terminal extension comprises at least one histidine residue that is capable of establishing a salt bridge with the lysine cleavage site N-terminal to the GLP-1.