Patent ID: 8980560
Filing Date: 2015-03-17
Classification: C12Q

Abstract:
1. A method for detecting DNA methylation comprising steps of: subjecting DNA to bisulfite treatment; subjecting the DNA after bisulfite treatment to a first PCR using the first set of primers corresponding to outer regions of the target region, the first set of primers selected from the group consisting of SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 30, and SEQ ID NO: 31; subjecting the amplified DNA after the first PCR to a second PCR using the second set of primers corresponding to the target region, the second set of primers selected from the group consisting of SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO 10, SEQ ID NO: 11, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 32, and SEQ ID NO: 33; and subjecting the amplified DNA after the second PCR to denaturing gradient gel electrophoresis, wherein the annealing positions of the second set of primers are located inside the annealing positions of the first set of primers relative to the target region.