Patent ID: 6365360
Filing Date: 2002-04-02
Classification: A61K,C07K

Abstract:
A method of determining whether a test compound modulates a signal transduction activity of a prostaglandin receptor IP, comprising:a) culturing IP-receptor-expressing host cells under conditions that would allow expression of a recombinant prostaglandin receptor, said host cells being transfected with a nucleic acid molecule encoding said recombinant prostaglandin IP-receptor comprising the amino acid sequence as set forth in SEQ ID NO:3; b) exposing the IP receptor-expressing host cells of step a) to the test compound; c) exposing control host cells to the test compound of step b), wherein said control host cells do not express recombinant prostaglandin IP receptor protein; d) measuring the modulating affect of the test compound which interacts with the recombinant IP receptor from the host cells of step a) and control host cells of step c); and, e) comparing the modulating affect of the test compound on the host cells and control host cells.