Patent ID: 7892819
Filing Date: 2011-02-22
Classification: B01D,B01F,B01J,B01L,C12M,C12Q,C40B,Y10S,Y10T

Abstract:
1. A device for amplifying a polynucleotide in a sample by conducting a polynucleotide amplification reaction, the device comprising a solid substrate microfabricated to define: a sample inlet port; a mesoscale flow system comprising; a sample flow channel extending from said inlet port; and a polynucleotide amplification region of the device, in fluid communication with said flow channel, said region having at least one cross-sectional dimension of width or depth of about 0.1 to 1000 microns, at least said polynucleotide amplification region being permanently sealed with a cover; and an appliance for use in combination with said substrate, said appliance including a nesting site for receiving said substrate and a conduit in fluid communication with the inlet port of said substrate.