Patent ID: 8771683
Filing Date: 2014-07-08
Classification: A61P,C07K,C12N

Abstract:
1. A composition comprising an EN-apyrase, wherein the EN-apyrase is a soluble CD39L3 or a homolog thereof, which has a homogeneous N-terminus wherein more than 80% of the EN-apyrase molecules and homologs have the same N-terminus beginning with EVLP (positions 20-23 of SEQ ID NO:24) and wherein (a) the EN-apyrase has an average isoelectric point in the range of about 3.0 to about 4.5; and/or (b) the EN-apyrase has an in vivo half life in rabbits or pigs at least twice that of HEK sol-CD39L3-01, measured by apyrase assay, wherein the homolog of soluble CD39L3 is a sequence having 1 to 5 conservative substitutions with respect to positions 49-485 of SEQ ID NO:1 and/or is at least 80% identical to positions 49-485 of SEQ ID NO:1, and which retains ADP'ase and ATP'ase activity.