Patent ID: 7354710
Filing Date: 2008-04-08
Classification: B01J,C12Q,C40B

Abstract:
1. A biomolecular hybridization device comprising: a substrate having an aminosilanized surface permanently and covalently attached thereto; and an adsorbed monolayer formed from an about twice saturating amount of unmodified single-stranded oligonucleotides all of which are about 12 to 16 bases in length adsorbed to the aminosilanized surface as a saturated film of constrained oligonucleotides on the surface via direct non-covalent phosphate-surface adsorptive contact of substantially all phosphate groups of each oligonucleotide at a density of one phosphate group per about 1.5 square nanometers of surface, wherein each constrained oligonucleotide base plane is presented from the surface in a manner effective to dissociably hybridize to a complementary single-stranded nucleic acid with asymmetric, substantially non-helical base pairing without alteration of the oligonucleotide base plane presentation and without oligonucleotide phosphate group dissociation from the surface.