Patent ID: 6911329
Filing Date: 2005-06-28
Classification: C07K,C12N,C12P,C12R

Abstract:
1. A process for preparing D-pantothenic acid and/or a salt thereof, comprising: culturing a recombinant modified Coryneform bacterium for a time and under conditions suitable for producing D-pantothenic acid or a salt thereof, and collecting the D-pantothenic acid or a salt thereof; wherein the pox B gene is deleted in the recombinant modified Coryneform bacterium, wherein the poxB gene in the Coryneform bacterium prior to being deleted comprises SEQ ID NO:1, SEQ ID NO:4, a polynucleotide which hybridizes under stringent conditions to the full complement of SEQ ID NO:1 and which encodes a protein with pyruvate oxidase activity, or a polynucleotide which hybridizes under stringent conditions to the full complement of SEQ ID NO:4 and which encodes a protein with pyruvate oxidase activity, wherein the stringent conditions comprise washing in 5×SSC at a temperature of 68° C.