Patent ID: 7312064
Filing Date: 2007-12-25
Classification: A61K,A61P,C07K,C12N

Abstract:
1. A method for producing an immunogenic influenza virus for use in vaccines, said method comprising: (a) introducing a set of plasmids into a host cell, wherein each plasmid of said set comprises one viral genomic segment, and wherein viral cDNA corresponding to the viral genomic segment is inserted between an RNA polymerase I (pol I) promoter and a regulatory element for the synthesis of vRNA or cRNA with an exact 3′ end, which results in expression of vRNA or cRNA, which are in turn inserted between an RNA polymerase II (pol II) promoter and a polyadenylation signal, which results in expression of viral mRNA and a corresponding viral protein, wherein the expression of the full set of vRNAs or cRNAs and viral proteins results in assembly of an infectious influenza virus; and (b) measuring the immunogenicity of said infectious influenza virus.