Patent ID: 6855547
Filing Date: 2005-02-15
Classification: A01H

Abstract:
1. A method for cultivating Swertia in vitro comprising: contacting a Swertia explant with a first medium comprising a MS basal culture medium I, from about 0.5 mg/L to about 1.0 mg/L benzyladenine, from about 1% to about 3% by weight of sucrose, and at least one auxin selected from the group consisting of about 0.5 mg/L naphthalene acetic acid, from about 0.5 mg/L to about 1.0 mg/L indole acetic acid, and from about 0.5 mg/L to about 1.0 mg/L indole butyric acid to produce a primary explant; contacting the primary explant with a second medium comprising a MS basal culture medium I, about 1.0 mg/L indole acetic acid, about 1.0 mg/L gibberellic acid, and from about 1% to about 3% sucrose to produce a secondary explant; and contacting the secondary explant with a third medium comprising a MS basal culture medium I, from about 1% to about 3% sucrose, and at least one auxin selected from the group consisting of from about 1.0 mg/L to about 5.0 mg/L naphthalene acetic acid, from about 1.0 mg/L to about 5.0 mg/L indole acetic acid, and from about 1.0 mg/L to about 5.0 mg/L indole butyric acid to produce a tertiary explant.