Patent ID: 6534261
Filing Date: 2003-03-18
Classification: A01K,A61K,A61P,C07K,C12N,C12Q,G01N

Abstract:
A method of inhibiting transcription of an endogenous cellular gene in an isolated cell, the method comprising the steps of:administering to the cell a nucleic acid molecule comprising a polynucleotide sequence which encodes a first zinc finger protein, wherein said first zinc finger protein is engineered, wherein said polynucleotide sequence is operably linked to a promoter, and wherein the nucleic acid molecule expresses the first zinc finger protein in the cell; and contacting a first target site in the endogenous cellular gene with the first zinc finger protein, wherein the Kd of the zinc finger protein is less than about 25 nM; thereby inhibiting transcription of the endogenous cellular gene by at least about 20%.