Patent ID: 9206410
Filing Date: 2015-12-08
Classification: C12N,C12Y

Abstract:
1. A method for preparing a recombinant plasminogen having a single kringle domain, a peptide linker sequence, an activation site, and a serine protease domain, wherein said single kringle domain is at least 90% identical to kringle 1 or kringle 4 of SEQ ID NO: 2; said peptide linker sequence between said single kringle domain and said serine protease domain consists of residues 91-94 of SEQ ID NO: 1; and said method comprises: contacting a composition comprising said recombinant plasminogen with a cation-exchange medium under a cation-exchange condition that is sufficient for said cation-exchange medium to bind said recombinant plasminogen; wherein said cation-exchange medium comprises a sulfopropyl ligand coupled to an agarose support.