Patent ID: 8574904
Filing Date: 2013-11-05
Classification: A61K,A61P,C12N

Abstract:
1. A method for producing a tooth comprising: culturing mouse or human pluripotent stem cells in the presence of a differentiation inducer to produce a cell population after differentiation induction treatment, said cell population containing CD44-positive and CD29-positive cells and/or CD44-positive and CD106-positive cells; selecting, from said cell population after the differentiation induction treatment, the CD44-positive and CD29-positive cells and/or the CD44-positive and CD106-positive cells as mesenchymal cells for the formation of the tooth, or obtaining the CD44-positive and CD29-positive cells and/or the CD44-positive and CD106-positive cells cultured from mouse or human pluripotent stem cells in the presence of a differentiation inducer; positioning, in a support carrier, a first cell mass consisting essentially of only either one of the mesenchymal cells or epithelial cells derived from a tooth germ and a second cell mass consisting essentially of only the other one of the mesenchymal cells or the epithelial cells derived from a tooth germ, said first and second cell masses being not mixed with each other but made to closely contact with each other; and culturing said first and second cell masses in said support carrier.