Patent ID: 6905822
Filing Date: 2005-06-14
Classification: C12N,C12Q

Abstract:
1. A method of determining the ability of a Mycobacterium tuberculosis bacterium to oxidize (a) ethionamide, said method comprising detecting a mutation in an EtaA gene (SEQ ID NO:1) in said bacterium, which mutated gene encodes an amino acid sequence which differs from that of SEQ ID NO:2 by (b) thiacetazone, said method comprising detecting a mutation in the EtaA gene (SEQ ID NO:1) in said bacterium, which mutated gene encodes an amino acid sequence which differs from that of SEQ ID NO:2 by (c) thiocarlide, said method comprising detecting a mutation in the EtaA gene (SEQ ID NO:1) in said bacterium, which mutated gene encodes an amino acid sequence which differs from that of SEQ ID NO:2 by wherein detection of the mutation is indicative of decreased ability to oxidize ethionamide, thiacetazone or thiocarlide, respectively.