Patent ID: 9133447
Filing Date: 2015-09-15
Classification: C12N,C12Q

Abstract:
1. A nucleic acid molecule encoding a DNase or an enzymatically active fragment thereof, said DNase having the sequence of SEQ ID No. 1 or a sequence which is at least 90% identical thereto, but wherein the proline residue at position 237 of SEQ ID No. 1, or the equivalent proline in other sequences, has been modified or substituted, said DNase or enzymatically active fragment thereof being substantially irreversibly inactivated by heating at a temperature of about 50° C. for 5 mins in a buffer consisting of 25 mM Tris HCl, pH 8.5, 5 mM MgCl 2 and 1 mM DTT, and being substantially specific for double stranded DNA.