Patent ID: 7521041
Filing Date: 2009-04-21
Classification: A61K,A61P,C07D

Abstract:
1. A method of determining the effect of antagonizing a muscarinic receptor on a biological system or sample comprising a muscarinic receptor, the method comprising: (a) contacting a first sample of the biological system or sample with a muscarinic agonist; (b) measuring the amount of cyclic adenosine monophosphate (cAMP) produced in the first sample; (c) contacting a second sample of the biological system or sample with the muscarinic agonist and a muscarinic receptor-antagonizing amount of a compound; (d) measuring the amount of cAMP produced in the second sample; and (e) comparing the amount of cAMP in step (b) with the amount in step (d); wherein the compound in step (c) has the formula: wherein: a is 0 or 1; R b is 0 or 1; R m is 0 or 1; R s is 0, 1 or 2; Ar t is 0, 1 or 2; n is 0 or an integer from 1 to 3; p is 0 or 1; and R wherein each alkyl group in R