Patent ID: 6068979
Filing Date: 2000-05-30
Classification: C12Q,G01N,Y10S

Abstract:
A method for sequentially detecting a first and second target species in a sample suspected of containing the first and second target species by two sequential chemiluminescent reactions comprising:immobilizing the first and second target species on a solid support;contacting the immobilized first and second target species with a first specific binding partner for the first target species and a second specific binding partner for the second target species to thereby form a first binding pair and a second binding pair;providing a peroxidase enzyme as a label for the first binding partner and providing a second enzyme as a label for the second binding partner;reacting the first binding pair with a chemiluminescent peroxidase substrate and a peroxide compound to produce a first chemiluminescent signal;detecting the first target species by detecting the first chemiluminescent signal;reacting the second binding pair with a composition comprising a chemiluminescent substrate for the second enzyme and an inhibitor of the peroxidase enzyme to stop the first chemiluminescent signal and produce a second chemiluminescent signal; anddetecting the second target species by detecting the second chemiluminescent signal.