Patent ID: 7445941
Filing Date: 2008-11-04
Classification: B01L,G01N,Y10S,Y10T

Abstract:
1. An assay device comprising: a housing; a first device region formed by or within said housing for receiving a fluid sample; a second device region fluidly connected to said first device region, said second device region comprising one or more capture zones on a surface within said housing, each said capture zone comprising molecules of a first antibody or binding fragment thereof reactive with an analyte of interest and at least one analyte sensor configured and arranged to detect an electrochemical signal related to reaction of a detectable amount of said analyte of interest with molecules of said first antibody or binding fragment thereof and produce a measurable signal in response, said signal being mediated by an enzyme conjugated to a second antibody or binding fragment thereof reactive with said analyte of interest; and a labeled reagent species dissolvably disposed on a surface within said housing that is in fluid communication with said second device region, wherein molecules of said labeled reagent species comprise said enzyme conjugated to said second antibody or binding fragment thereof reactive with said analyte of interest, wherein molecules of said labeled reagent species form sandwich complexes with molecules of said analyte of interest and with molecules of said first antibody or binding fragment thereof.