Patent ID: 8940500
Filing Date: 2015-01-27
Classification: G01N

Abstract:
1. A method for identifying a metakaryotic stem cell of interest having a bell-shaped nucleus, the method comprising: a) fixing a sample of cells with an acid-alcohol mixture; b) treating said sample with a Feulgen's reagent with fuchsin as the Schiff's base; c) maintaining said sample in contact with the Feulgen's reagent in order to stain the sample in the absence of extraneous or exogenously added fluorescent dyes; d) exposing said stained sample to an excitation light from a light source; and e) visualizing green-light emitting fluorescent balloon-like cytoplasmic structures of a metakaryotic stem cell by fluorescence microscopy, thereby identifying the metakaryotic stem cell of interest in the sample.