Patent ID: 9186668
Filing Date: 2015-11-17
Classification: B01L,G01N

Abstract:
1. A method comprising: introducing a fluid sample containing a quantity of white blood cells into a channel defined in part by a substrate; forming complexes which include label moieties affixed to at least a portion of the quantity of white blood cells; providing first and second density media in separate chambers defined at least in part by the substrate wherein the first density media has a density different from that of the second density media and wherein the first and second density media have a density lower than a density of the white blood cells and higher than a density of the fluid sample; spinning the substrate to transport the first and second density media from the separate chambers into a detection region defined in part by the substrate; transporting the quantity of white blood cells toward the detection region comprising the layered density media; spinning the substrate such that at least a portion of the quantity of white blood cells is transported through at least a portion of the first or second density media; and detecting signals from the label moieties affixed to the at least a portion of the quantity of white blood cells, including detecting signals from label moieties near an interface of the first and second density media.