Patent ID: 8835106
Filing Date: 2014-09-16
Classification: C12Q

Abstract:
1. A method for identifying a HBV strain resistant to lamivudine and penciclovir in a sample of serum of a HBV-infected patient, comprising: (i) amplifying at least a part of the HBV gene present in said sample with at least one primer pair; (ii) hybridizing the polynucleic acid products of the amplification of (i) with at least one probe which hybridizes specifically under stringent conditions to the codon coding for V of the YVDD motif of the HBV RT pol gene target sequence comprising the codon coding V of the YVDD motif; wherein said probe hybridizing specifically under stringent conditions to a HBV RT pol gene target sequence comprising the codon coding for the V of the YVDD motif is selected from the group consisting of SEQ ID NOs: 101-103, 116, 117, 130-133, 244-248, 251-260 and 262-266, or wherein said probe hybridizes specifically under stringent conditions to a complement of a sequence selected from the group consisting of 101-103, 116, 117, 130-133, 244-248, 251-260, and 262-266; (iii) based on the results of hybridizing (ii), determining the presence or absence of a mutation in the HBV RT pol gene resulting in an amino acid change in the YMDD motif of the HBV polymerase wherein said amino acid change is M to V in the YMDD motif and thereby identify the existence of a HBV strain resistant to lamivudine and penciclovir in said sample.