Patent ID: 9181566
Filing Date: 2015-11-10
Classification: C12P,C12Y,Y02E

Abstract:
1. A method of producing butanol or 2-butanone comprising: A) contacting a recombinant host cell comprising a heterologous polynucleotide, said heterologous polynucleotide comprising i) a promoter nucleic acid sequence, wherein the promoter nucleic acid sequence is selected from SEQ ID NO: 775, 776, 777, 778, 772, 773, 768, 769, or 711; and ii) a nucleic acid sequence encoding a biocatalyst polypeptide, wherein the biocatalyst polypeptide catalyzes a substrate to product conversion of pyruvate to acetolactate; with a carbon substrate under a first set of conditions; and B) contacting the recombinant host cell with a carbon substrate under a second set of conditions; wherein the first set of conditions and second set of conditions differ and wherein the nucleic acid sequence encoding a biocatalyst polypeptide is differentially expressed under the first set of conditions than under the second set of conditions; and wherein the host cell produces butanol or 2-butanone under at least one of the first set or the second set of conditions.