Patent ID: 6825015
Filing Date: 2004-11-30
Classification: A61P,C12P

Abstract:
A process for the isolation and purification of HMG-CoA reductase inhibitors from mycelium biomass which comprises:clarifying a mycelium broth and concentrating the clarified broth to a lower volume, acidifying the concentrate to a pH value in the range of 4.5 to 7.5, followed by extracting the HMG-CoA reductase inhibitor with ethyl acetate; optionally performing lactonization; crystallizing the HMG-CoA reductase inhibitor from: i) a water-miscible first organic solvent; and ii) ii) a second organic solvent having limited miscibility or solubility with water selected from the group consisting of n-butanol, isobutanol, amyl alcohol, hexanol, 2-ethylhexanol, benzyl alcohol, cyclohexanol, methylbutyl ketone, methyl isobutyl ketone, cyclohexanone, methyl acetate, ethyl acetate, n-propyl acetate, isopropyl acetate, isobutyl acetate, sec-butyl acetate, amyl acetate, diethyl ether, diisopropyl ether, methylene chloride, chloroform, and mixtures of these solvents.