Patent ID: 7423140
Filing Date: 2008-09-09
Classification: C07D,C07H,C09B,C12Q

Abstract:
1. A fluorescently-labeled reagent comprising an oligonucleotide or an oligonucleotide analog and an energy transfer dye, wherein energy transfer dye is covalently linked to the oligonucleotide or oligonucleotide analog, and comprises: a fluorescein donor dye capable of absorbing light at a first wavelength and emitting excitation energy in response thereto; a 4,7-dichlororhodamine acceptor dye capable of absorbing the excitation energy emitted by the donor dye and fluorescing at a second wavelength in response thereto; and a non-nucleosidic linker linking the 5- or 6-position of the fluorescein donor dye to the 5- or 6-position of the 4,7-dichlororhodamine acceptor dye.