Patent ID: 8553732
Filing Date: 2013-10-08
Classification: A61B,G01N

Abstract:
1. A method of determining a disease state of mammalian cell, the method comprising: irradiating a sample that includes the cell with substantially monochromatic light having a wavelength not greater than 695 nanometers, wherein said irradiating further comprises irradiating said sample using wide-field illumination; assessing Raman scattered light emitted by the cell at an Raman shift value selected from the group consisting of Raman shift values in the range from 280 to 1800 cm comparing the intensity of the Raman scattered light emitted by the cell with a reference value corresponding to the intensity of Raman scattered light emitted by a reference cell of the same type, whereby a difference between the intensity of the Raman scattered light emitted by the cell and the reference value is indicative of the disease state of the cell, wherein said comparing is achieved by applying at least one chemometric technique; generating a digital brightfield image of the Raman scattered light emitted by the cell; and combining a digital brightfield image of the cell and the Raman scattered light emitted by the cell, whereby the Raman scattered light emitted by the cell is informative of the disease state of the cell.