Patent ID: 6316692
Filing Date: 2001-11-13
Classification: A01K,A61K,A61P,C12N

Abstract:
An in vivo method of incorporating a polynucleotide into germ cells of a male non-human mammal for the production of transgenic non-human mammals, comprising:administering by injection into a testis of a male non-human mammal a transfection mixture comprising at least one polynucleotide encoding a gene product in operable linkage with a promoter, and at least one transfecting agent, other than a liposome/DNA complex, wherein said testis contains the germ cells of the male non-human mammal, and wherein said germ cells are selected from the group consisting of spermatogonial stem cells, type B spermatogonia, primary spermatocytes, preleptotene spermatocytes, leptotene spermatocytes, zygotene spermatocytes, pachytene spermatocytes, secondary spermatocytes, spermatids, and spermatozoa; andallowing the polynucleotide encoding a gene product to be taken up by, and released into, the germ cells so that the released polynucleotide is incorporated into the genome of the germ cells of said male non-human mammal thereby producing a transgenic non-human mammal.