Patent ID: 6063575
Filing Date: 2000-05-16
Classification: A61K,C07K,G01N,Y02A

Abstract:
A method for identifying whether a test compound alters the fidelity of a multi-protein DNA replication complex comprising:(A) exposing said test compound to (i) a purified multi-protein DNA replication complex isolated from a mammalian cell and capable of supporting all phases of origin-specific DNA replication in vitro, and (ii) a DNA template comprising an origin of replication recognized by said complex, under conditions to achieve replication of said DNA template;(B) detecting DNA mutations in the replicated DNA template obtained in step (A) and determining the frequency of DNA mutation in said replicated DNA template; and(C) comparing the frequency of DNA mutation occurrence in the presence of said test compound with the frequency of spontaneous DNA mutation occurrence and with the frequency of DNA mutation occurrence in a negative control.