Patent ID: 7993853
Filing Date: 2011-08-09
Classification: C12Q,Y10T

Abstract:
1. A method for isolating a target nucleic acid of interest from a sample, comprising: mixing a sample containing a target nucleic acid with a capture probe that hybridizes specifically to a target sequence in the target nucleic acid in a detergent-containing solution phase that contains imidazole in a concentration range of 0.5 M to about 4.2 M and an immobilized probe that binds specifically to the capture probe, to provide a reaction mixture, incubating the reaction mixture at a first temperature in a range of about 85° C. to 95° C. for about 15 minutes or less, incubating the reaction mixture at a second temperature in a range of about 25° C. to 42° C. for about 20 minutes or less, thereby forming a hybridization complex made up of the capture probe hybridized specifically to the target nucleic acid and the immobilized probe bound specifically to the capture probe, wherein the hybridization complex is attached to a support via the immobilized probe, and separating the hybridization complex attached to the support from other sample components.