Patent ID: 6555698
Filing Date: 2003-04-29
Classification: C07H,C12Q,G01N

Abstract:
An enzymatically cleavable chemiluminescent 1,2-dioxetane of formula I: which is capable of producing light energy when decomposed;wherein T is a substituted or unsubstituted polycycloalkyl group bonded to the 4-membered ring portion of said dioxetane by a Spiro linkage; wherein X is an aryl or heteroaryl moiety of 6-30 carbon atoms which induces chemiluminescent decomposition of said 1,2-dioxetane upon enzymatic cleavage of said moiety Z; wherein Z is an enzymatically cleavable group of formula II: â€ƒwherein R1-R3 are each independently H or an alkyl (branched or straight chain) of 1-4 carbon atoms, and wherein R is an alkyl, aryl, aralkyl or cycloalkyl of 1-20 carbon atoms.