Patent ID: 7642053
Filing Date: 2010-01-05
Classification: C12Q

Abstract:
1. A method for PCR amplification and detection of nucleotide sequences, comprising: using a hydrophilic reaction layer having coupling groups for covalent binding of probe molecules and an array of a plurality of microspots forming analytical positions, said microspots including, as probe molecules, at least one immobilized oligonucleotide which is hybridizable with a target sequence to be identified of a DNA fragment; applying an analyte solution including PCR reagents and a plurality of target sequences to the microspots such that the analyte solution completely covers the array; subjecting the array to a thermocycling process to amplify the target sequences; and detecting hybridization events on the probe molecules immobilized at one of the analytical positions electrochemically with the aid of a microelectrode arrangement wherein detected nucleotide sequences alter impedance of the microelectrode arrangement and a label is excluded from the detected nucleotide sequences.