Patent ID: 7741078
Filing Date: 2010-06-22
Classification: A61K,A61P,C07K

Abstract:
1. A method of producing a heparin-binding protein having at least one sulfated glycosaminoglycan sugar chain covalently bonded thereto, wherein 90% or more of said at least one sulfated glycosaminoglycan sugar chain being a heparan sulfate sugar chain, wherein the method comprises: (a) a step of ligating a cDNA encoding a peptide consisting of an amino acid sequence shown in SEQ ID NO: 8 which is capable of being modified with at least one heparan sulfate sugar chain to a cDNA encoding a heparin-binding protein; (b) a step of incorporating the resultant ligated cDNA into an expression vector; (c) a step of introducing the expression vector into a host cell having a pathway for heparan sulfate sugar chain biosynthesis; and (d) a step of expressing in the host cell a heparin-binding protein to which at least one heparan sulfate sugar chain is covalently bonded through the peptide consisting of the amino acid sequence shown in SEQ ID NO: 8.