Patent ID: 6905847
Filing Date: 2005-06-14
Classification: C12N

Abstract:
1. A method of determining the optimal level of product expression in animal cell culture wherein the concentration of a solute of interest in a culture medium composition for optimal product expression is different than the concentration of said solute in the culture medium composition determined for optimal cell growth, which method comprises: a) growing the animal cell culture in a culture medium to determine optimal cell growth; b) varying the concentration of the solute in the culture medium to a concentration above that optimal for cell growth, which concentration is effective to create an environment of solute stress on the cell culture as expressed by an inhibitory effect on cell growth or cell density of said cell culture; c) monitoring the product expression as concentration of the solute is varied in the culture medium to determine optimal product expression; and d) selecting the solute concentration that provides the optimal combination of cell growth and product expression, which allows for optimal productivity.