Patent ID: 8697354
Filing Date: 2014-04-15
Classification: C12N,C12Q,G01N

Abstract:
1. A method for identifying an agent that inhibits an activity of a bacterial RNAP by binding to a homologous RNA-exit-channel amino-acid sequence of a bacterial RNAP, comprising: (a) preparing a reaction solution comprising the agent to be tested and a bacterial RNAP that contains a homologous RNA-exit-channel amino-acid sequence; (b) detecting at least one of the presence, extent, concentration-dependence, or kinetics of inhibition of an activity of said bacterial RNAP in the reaction solution, by binding of the agent to the homologous RNA-exit-channel amino-acid sequence of the bacterial RNAP; (c) using the information obtained in step (b) to identify one or more agents that inhibit an activity of the bacterial RNAP by binding to the homologous RNA-exit channel amino-acid sequence of the bacterial RNAP, wherein the agent is not lipiarmycin.