Patent ID: 9017992
Filing Date: 2015-04-28
Classification: A61K,C07C,C07H,C08G,C12Q,G01N

Abstract:
1. A composition comprising a recognition unit, a detection unit, and an adaptor unit, wherein: a) the recognition unit and the detection unit each comprises a hybridizing nucleic acid analog segment and the adaptor unit comprises a first hybridizing nucleic acid analog segment and a second hybridizing nucleic acid analog segment; b) the hybridizing nucleic acid analog segment of the recognition unit specifically hybridizes to the first hybridizing nucleic acid analog segment of the adaptor unit and the second hybridizing nucleic acid analog segment of the adaptor unit specifically hybridizes to the hybridizing nucleic acid analog segment of the detection unit; c) the recognition unit further comprises at least one probe which recognizes at least one target in a sample; d) the detection unit further comprises at least one detectable label; e) the hybridizing nucleic acid analog segment on the recognition unit does not specifically hybridize to the target; f) the recognition unit does not specifically hybridize to the detection unit and does not specifically hybridize to the probe; g) the detection unit does not specifically hybridize to the recognition unit, does not specifically hybridize to the detectable label, and does not specifically hybridize to the target; h) the adaptor unit does not specifically hybridize to the target, does not specifically hybridize to the detectable label, and does not specifically hybridize to the probe; and i) the nucleic acid analog segments on the recognition unit, adaptor unit, and detection unit each comprise at least one base chosen from a thio-xanthine, pyrimidinone, 2-thio-guanine, and 2-amino-6-thioxopurine.