Patent ID: 6770447
Filing Date: 2004-08-03
Classification: B01L,Y10S

Abstract:
A method for detecting the presence or amount of an analyte of interest in a test sample, comprising:providing an optical assay device, said optical assay device comprising a base comprising absorbent material, and a member comprising an optically active test stack, said member rotatably coupled to said base for rotation between a lowered position where said optically active test stack contacts said absorbent material and a raised position where said optically active test stack does not contact said absorbent material; applying said test sample to said optically active test stack, wherein said member is in said lowered position; applying a conjugate that binds to the analyte on to said optically active test stack; applying a wash to said optically active test stack; rotating said member to said raised position; applying an amplifying reagent solution that amplifies a visual indication of the presence or amount of said analyte on said optically active test stack; rotating said member to said lowered position; and observing said optically active test stack for the visual indication of the presence or amount of the analyte of interest.