Patent ID: 8217015
Filing Date: 2012-07-10
Classification: A61K,C12N

Abstract:
1. A method for delivering a polynucleotide to the cytoplasm of a cell comprising: a) forming a first membrane active polyvinylether polymer having the formula:  wherein R1 contains an amine group, R2 is an alkyl or aryl group, and x and y are integers greater than zero; b) forming a second membrane active polyvinylether polymer capable of causing liposome leakage having the formula:  wherein R1 contains an amine group, R2 is an alkyl or aryl group, and x and y are integers greater than zero; c) reversibly modifying the second membrane active polyvinylether polymer via covalent linkage of a plurality of carboxydimethylmaleic anhydrides to amines on the polymer thereby forming a negatively charged reversibly inhibited membrane active polymer, wherein: d) associating said polynucleotide with the first amine-containing amphiphilic polyvinylether polymer to form a binary complex; e) associating said binary complex with the reversibly inhibited membrane active polymer to form a ternary complex; and f) contacting the cell with the ternary complex resulting in delivery of the polynucleotide to the cell.