Patent ID: 7217534
Filing Date: 2007-05-15
Classification: A61P,G01N

Abstract:
1. A method of screening cellular polypeptides for pro-apoptotic or anti-apoptotic activity in a cell of a particular cell-type, said method comprising a. culturing cells of said particular cell-type under non apoptotic conditions and culturing cells of said particular cell-type under apoptotic conditions; and, b. detennining subcellular localisation of said cellular polypeptides in the cultured cells; wherein a localization of a cellular polypeptide in lipid rafts in cultured cells under non apoptotic conditions and a segregation of said cellular polypeptide from lipid rafts in cultured cells under apoptotic conditions is indicative that said cellular polypeptide has a pro-apoptotic or an anti-apoptotic activity in said particular cell-type, and wherein said screened cellular polypeptides belong to the Bcl-2 family and their subcellular localisation is determined by the use of a molecule which specifically recognizes a BH domain.