Patent ID: 8597950
Filing Date: 2013-12-03
Classification: C12N

Abstract:
1. A system for replicating or for replicating and expressing a sequence of interest in a plant, comprising: (i) a DNA precursor of an RNA replicon, said RNA replicon derived from a tobamovirus and comprising at least one sequence of interest, said DNA precursor of said RNA replicon containing in a tobamoviral replicase open reading frame (ORF) or in a tobamoviral movement protein ORF of said RNA replicon, one or more introns inserted within A/U rich localities of said tobamoviral replicase ORF or said tobamoviral movement protein ORF; and (ii) a DNA precursor of a helper replicon, wherein said helper replicon is whereby said RNA replicon (i) is capable of replication or of replicating and expressing said sequence of interest in a plant, but unable to move systemically in a plant in the absence of said one or more proteins expressed by said helper replicon (ii).