Patent ID: 8940535
Filing Date: 2015-01-27
Classification: A61K,A61P,C12N

Abstract:
1. A method of isolating CD34+ stem cells from a plurality of bone marrow cells, comprising the steps of: culturing bone marrow cells in a first liquid cell culture medium that lacks serum, but that comprises interleukin-3, interleukin-6, stem cell factor, and beta-mercaptoethanol; successively passaging cells in suspension from the first medium to a subsequent liquid cell culture medium that lacks serum, but that comprises interleukin-3, interleukin-6, stem cell factor, and beta-mercaptoethanol, wherein the passaging occurs about once a week or more than once a week and the successive passaging steps occur over the course of three to four weeks; and isolating the cells in suspension from the final medium.