Patent ID: 6645488
Filing Date: 2003-11-11
Classification: A61K,C12N

Abstract:
A microencapsulated medicine of ox adrenal medulla pheochromocyte for treating pain, which is prepared through the following steps:(1) mixing the ox adrenal medulla pheochromocyte with 10-20 g/L solution of sodium alginate to form suspension, which contains 0.1-1Ã—1010 cells per liter said suspension, (2) dispersing the suspension of step (1) into 80-120 mmol/L calcium chloride solution or calcium lactate solution in the state of microdrops of diameter of 150-1000 &mgr;m by spray device, the proportion of the two solution is to ensure 1 liter mixture to contain 0.1-1Ã—108 cells, setting 5-20 minutes, removing the supernatant after depositing completely, then obtaining the calcium alginate bead deposit which contains ox adrenal medulla pheochromocytes; (3) adding the deposit derived from step (2) into the 0.3-0.7 g/L polylysine solution, the proportion of the two solution is to ensure 1 liter mixture contains 0.2-2Ã—108 cells, mixing uniformly, setting 5-20 minutes, removing the supernatant after depositing completely, getting the deposit; (4) adding the deposit obtained from step (3) into 1.0-2.0 g/L sodium alginate solution, the proportion of the two solution is to ensure 1 liter mixture contains 0.2-2Ã—108 cells, mixing it uniformly, setting 3-15 minutes, removing the supernatant after depositing completely, getting the deposit; (5) adding the deposit obtained from step (4) into 40-70 mmol/L sodium citrate solution, the proportion of the two solution is to ensure 1 liter mixture contains 0.2-2Ã—108 cells, mixing it uniformly, setting 5-20 minutes, removing the supernatant after depositing completely, getting the microencapsulated pheochromocyte deposit of ox adrenal medulla; (6) washing the deposit obtained from step (5) by adding it into the 9 g/L soludium chloride solution, finally transferring the deposit into a cell culture, and storing it as microencapsulated medicine of ox adrenal medulla pheochromocyte.