Patent ID: 6528064
Filing Date: 2003-03-04
Classification: A61K,A61P,C07K,C12N

Abstract:
A reassortant influenza virus comprising:(1) a RNA sequence encoding an influenza Type A HA glycoprotein having a sequence corresponding to an HA sequence of an epidemic influenza A virus strain, (2) a RNA sequence encoding an influenza Type A NA glycoprotein having a sequence corresponding to a NA sequence of an epidemic influenza A virus strain, (3) a RNA sequence encoding an influenza Type A PB2 protein, said RNA sequence being modified to encode a substitution of at least one non-native amino acid for at least one native amino acid that affects the cap-binding activity of PB2 in the region spanning amino acid residues 537 through 575, and (4) RNA sequences encoding influenza Type A PB1, PA, NP, M and NS proteins, which RNA sequences correspond to sequences of a transfectant, non-epidemic influenza A virus strain.