Patent ID: 6346379
Filing Date: 2002-02-12
Classification: C12N

Abstract:
A recombinant thermostable DNA polymerase which is characterized in thata) in its native form said polymerase comprises the amino acid sequence LeuSerXaaXaaLeuXaaXaaProXaaXaaGlu (SEQ ID NO: 1), whereby â€œXaaâ€  at positions 3, 4, 6, 9, and 10 of said sequence are any amino acid residue, and â€œXaaâ€  at position 7 of said sequence is Val or Ile; b) said â€œXaaâ€  at position 4 is mutated in comparison to the native sequence to Lys; and c) said thermostable DNA polymerase has a level of discrimination against incorporation of nucleotides labeled with fluorescein family dyes which is reduced in comparison to the native form of the polymerase.