Patent ID: 7029861
Filing Date: 2006-04-18
Classification: C07K,C12Q,G01N

Abstract:
1. A method of screening for modulators of a lipopolysaccharide mediated response comprising the steps of: a) obtaining a cell expressing a TLR-4 polypeptide, wherein the TLR-4 polypeptide has the amino acid sequence of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:98 or SEQ ID NO:99, or is encoded by a nucleic acid sequence comprising SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:46, SEQ ID NO:47 or SEQ ID NO:48; b) measuring a lipopolysaccharide mediated response mediated by the TLR-4 polypeptide; c) contacting the TLR-4 polypeptide with a putative modulator; d) assaying for a change in the lipopolysaccharide mediated response; and e) comparing the lipopolysaccharide mediated responses mediated by the TLR-4 polypeptide obtained in steps b) and d) above wherein a difference in the lipopolysaccharide mediated responses indicates that the putative modulator is a modulator of a lipopolysaccharide mediated response.