Patent ID: 9017972
Filing Date: 2015-04-28
Classification: C12Q

Abstract:
1. A method for identifying FoxP3-positive CD25 + CD4 + regulatory T cells (Tregs) of a human subject, the method comprising: Isolating genomic DNA from a biological sample from the subject, and treating the genomic DNA with bisulfite, detecting, in the bisulfite treated genomic DNA, the methylation status of at least one CpG position in the region of the foxp3 gene consisting of SEQ ID NO: 1; wherein said detecting the methylation status comprises creating an amplicon by amplification with at least one methylation-specific primer having the sequence of SEQ ID NO: 14 or 16, and at least one non-methylation-specific primer having the sequence of SEQ ID NO: 15 or 17, and hybridizing the resulting amplicon with at least one suitable methylation-specific probe and/or at least one suitable non-methylation-specific probe; determining that said at least one CpG position is demethylated to at least 90%: and correlating the detected extent of demethylation of said at least one CpG position with an identification the sample as having FoxP3-positive CD25