Patent ID: 9175335
Filing Date: 2015-11-03
Classification: B82Y,C12Q,G01Q,Y10T

Abstract:
1. A method for determining the presence of a mismatched nucleotide between a probe nucleotide and a target nucleotide, said method comprising: comparing a control atomic force microscopy (AFM) binding force with a measured AFM binding force between a probe nucleotide and a target nucleotide to determine whether there is a mismatch between said probe nucleotide and said target nucleotide, wherein said AFM comprises a cantilever having a fixed end and a free end, wherein the free end of said cantilever comprises a tapered protrusion of surface region, and wherein said probe nucleotide is attached to said cantilever via a dendron comprising: branched regions, and a single linear region, and wherein a plurality of termini of said branched regions of dendron are covalently bound to the surface region of said cantilever, and said probe nucleotide is attached to the terminus of the single linear region of said dendron, wherein said dendrons are spaced at regular intervals between about 0.1 nm and about 100 nm.