Patent ID: 8450055
Filing Date: 2013-05-28
Classification: A61K,A61P,C12N,G01N,Y02A

Abstract:
1. A method of identifying an potential antigen from a Plasmodium species, which method comprises a) preparing an adenoviral vector array comprising two or more different adenoviral vectors, wherein each adenoviral vector comprises a nucleic acid sequence encoding a different polypeptide of a (b) contacting antigen presenting cells (APCs) with the adenoviral vector array, wherein each different adenoviral vector transduces an APC such that the nucleic acid sequences of the different adenoviral vectors are expressed and products of said expression are displayed by the APCs, (c) incubating the APCs with effector T lymphocytes obtained from a mammal immunized with a (d) screening for an immune response from the effector T lymphocytes whereupon the immunogenicity against said polypeptide from step (a) is identified as antigenic, and (e) recovering said nucleic acid sequence encoding said identified antigenic polypeptide from the adenoviral vector, wherein the polypeptide encoded by said recovered nucleic acid sequence is identified as an potential antigen of a