Patent ID: 7550276
Filing Date: 2009-06-23
Classification: C12Q

Abstract:
1. A method of simultaneously detecting or quantifying n kinds of different target nucleic acids in a specimen, wherein each of the n kinds of different target nucleic acids contains a first partial sequence Fa and a second partial sequence Sa and is set forth as Fa-Sa, wherein said Fa is one of sequences F 1 to Fn, and said Sa is one of sequences S 1 to Sn, wherein n is an integer of 2 or more comprising: (a) preparing different nucleic acid probes A (b) mixing said probes A (c) ligating said probes A (d) binding the first binding molecule of said probes A (e) obtaining n kinds of different flag sequences by isolating each said flag sequence from said ligated probes A (f) amplifying the n kinds of different flag sequences by polymerase chain reaction (PCR), wherein the PCR uses a primer labeled with a marker substance and the n kinds of different flag sequences as templates, and thereby obtaining n kinds of different amplification products labeled with the marker substance; and (g) detecting or quantifying the n kinds of different amplification products labeled with the marker substance, and thereby detecting or quantifying the n kinds of different target nucleic acids in the specimen.