Patent ID: 8927701
Filing Date: 2015-01-06
Classification: C12N,C12Q

Abstract:
1. A kit for diagnosis of a respiratory disease or infection, comprising: a first primer, the nucleotide sequence of which is a DNA sequence consisting of the first 14-30 nucleotides at the 5′ end of the sequence selected from the group consisting of SEQ ID NOs: 62-66; a second primer, the nucleotide sequence of which is a DNA sequence consisting of the sequence that is complementary to the last 14-30 nucleotides at the 3′ end of the selected sequence, wherein said first primer and said second primer form a primer pair, which amplifies nucleic acid from each of the five HPIV-2 viruses deposited at Collection Nationale de Cultures de Microorganismes, accession numbers I-3761, I-3762, I-3763, I-3764 and I-3765; and a first probe, which can be used in real-time amplification with said primer pair, wherein the nucleotide sequence of said first probe is a DNA sequence comprising the nucleotide sequence of SEQ ID NO: 90 or the complementary sequence thereof, and wherein said first probe detects each of said five deposited HPIV-2 viruses, without detecting the Greer, Toshiba and V98 isolates.