Patent ID: 7407677
Filing Date: 2008-08-05
Classification: A21D,C12N,C12P,C12Y,Y02E

Abstract:
1. An isolated, synthetic or recombinant nucleic acid comprising: (a) a nucleic acid sequence encoding a polypeptide having alpha amylase activity, wherein the nucleic acid sequence has at least 95% sequence identity to the sequence of SEQ ID NO:125, (b) a nucleic acid sequence encoding a polypeptide having alpha amylase activity, wherein the polypeptide comprises (i) an amino acid sequence having at least 95% sequence identity to the sequence of SEQ ID NO:126, or (ii) enzymatically active fragments of (i); (c) the nucleic acid of (a) or (b) encoding a polypeptide having at least one conservative amino acid substitution as compared to the polypeptide encoded by (a) or (b), wherein the conservative amino acid substitutions comprise: replacement of an aliphatic amino acid with another aliphatic amino acid; replacement of a Serine with a Threonine or vice versa; replacement of an acidic residue with another acidic residue; replacement of a residue bearing an amide group with another residue bearing an amide group; exchange of a basic residue with another basic residue; or replacement of an aromatic residue with another aromatic residue; (d) the nucleic acid of (a), (b) or (c) encoding a polypeptide lacking a signal sequence; (e) the nucleic acid of (a), (b), (c) or (d) further comprising a heterologous sequence; (f) the nucleic acid of (e), wherein the heterologous sequence comprises a sequence encoding a heterologous signal sequence; (g) the nucleic acid of (e), wherein the heterologous sequence comprises a sequence encoding an N-terminal identification peptide; or (h) sequences completely complementary to (a), (b), (c), (d), (e), (f) or (g).