Patent ID: 8486625
Filing Date: 2013-07-16
Classification: C12Q

Abstract:
1. A method of detecting a plurality of target nucleic acid sequences, comprising: a) providing one or more first solid supports comprising a plurality of target sequences that are immobilized to said first solid supports, wherein said first solid supports comprise a plurality of beads; b) hybridizing a plurality of different first primers to first portions of said plurality of target sequences, wherein each of said different first primers comprises an adapter sequence exogenous to said target sequences; c) hybridizing a plurality of different second primers to second portions of said plurality of target sequences, thereby forming a plurality of hybridization complexes; d) extending said first or said second primers, and ligating said first and second primers together to form a plurality of different modified primers; e) hybridizing said adapter sequences of said modified primers or their complements to an array of capture probes attached to discrete sites on a second solid support; f) modifying said capture probes by polymerase extension, and g) detecting said modified capture probes, thereby detecting said plurality of target nucleic acid sequences.