Patent ID: 7820385
Filing Date: 2010-10-26
Classification: C12Q

Abstract:
1. A method for preserving information about global genomic cytosine methylation status in an amplified genome, comprising: contacting a genomic sample with a modifying agent comprising bisulfite that converts unmethylated cytosine residues but not methylated cytosine residues to produce a converted genomic sample, wherein the converted cytosine residues present in the converted genomic sample are distinguishable from the methylated cytosine residues to provide the information about global genomic cytosine methylation status; and contacting the converted genomic sample with a DNA polymerase to amplify the converted genomic sample by multiple strand displacement amplification under isothermal conditions, thereby producing an amplified genome, wherein the converted genomic sample is not contacted with a nucleic acid ligase or an RNA polymerase, thereby preserving information about global genomic cytosine methylation status in the amplified genome.