Patent ID: 6395515
Filing Date: 2002-05-28
Classification: C07K,C12N

Abstract:
A method for producing a modified antibody heavy chain by switch-mediated recombination, the method comprising the steps of:a) introducing a targeting construct into an isolated B cell or B cell hybridoma that facilitates switch-mediated recombination, wherein the targeting construct comprises, in order from 5â€² to 3â€² and operably linked, a promoter, an antibody heavy chain variable region sequence, and a switch region (S1), and wherein an antibody heavy chain expressed by the cell is encoded by an antibody heavy chain target locus comprising, in order from 5â€² to 3â€² and operably linked, a promoter, an antibody heavy chain variable region, a switch region (S2), and an antibody heavy chain constant region;b) culturing the cell to allow transcription of the targeting construct, wherein switch-mediated recombination between said S1 and S2 is promoted; and c) selecting a cell comprising a modified target locus which comprises, in order from 5â€² to 3â€² and in operable linkage, the targeting construct promoter, the targeting construct heavy chain variable region, a switch region, and the target locus antibody heavy chain constant region; wherein a modified antibody heavy chain is produced.