Patent ID: 6265187
Filing Date: 2001-07-24
Classification: A61K,B82Y,C07K,F02B,G01N

Abstract:
An isolated DNA molecule encoding a recombinant endotoxin binding polypeptide selected from the group consisting of:(a) L.sub.1-59 B.sub.60-456 wherein L.sub.1-59 is the LBP sequence of amino acids corresponding to amino acids 1 to 59 of SEQ ID NO:2 and B.sub.60-456 is the BPI sequence of amino acids corresponding to amino acids 60 to 456 of SEQ ID NO:4 or 16;(b) L.sub.1-134 B.sub.135-456 wherein L.sub.1-134 is the LBP sequence of amino acids corresponding to amino acids 1 to 134 of SEQ ID NO:2 or 8 and B.sub.135-456 is the BPI sequence of amino acids corresponding to amino acids 135 to 456 of SEQ ID NO:4 or 16;(c) L.sub.1-275 B.sub.278-456 wherein L.sub.1-275 is the LBP sequence of amino acids corresponding to amino acids 1 to 275 of SEQ ID NO:2, 8 or 12 and B.sub.278-456 is the BPI sequence of amino acids corresponding to amino acids 278 to 456 of SEQ ID NO:4 or 16;(d) L.sub.1-359 B.sub.360-456 wherein L.sub.1-359 is the LBP sequence of amino acids corresponding to amino acids 1 to 359 of SEQ ID NO:2, 8 or 12 and B.sub.360-456 is the BPI sequence of amino acids corresponding to amino acids 360 to 456 of SEQ ID NO:4;(e) L.sub.1-197 B.sub.200-456 wherein L.sub.1-197 is the LBP sequence of amino acids corresponding to amino acids 1 to 197 of SEQ ID NO:2 or 12 and B.sub.200-456 is the BPI sequence of amino acids corresponding to amino acids 200 to 456 of SEQ ID NO:4 or 16;(f) L.sub.1-197(I43->V) B.sub.200-456(N206->D) wherein L.sub.1-197(I43->V) is the LBP sequence of amino acids corresponding to amino acids 1 to 197 of SEQ ID NO:2, 8 or 12, except that Ile at the position corresponding to amino acid 43 has been substituted by Val, and B.sub.200-456(N206->D) is the BPI sequence of amino acids corresponding to amino acids 200 to 456 of SEQ ID NO:4 or 16, except that Asn at the position corresponding to amino acid 206 has been substituted by Asp;(g) L.sub.1-199 B.sub.200-456(S351->A) wherein L.sub.1-199 is the LBP sequence of amino acids corresponding to amino acids 1 to 199 of SEQ ID NO:2, 8 or 12 and B.sub.200-456(S351->A) is the BPI sequence of amino acids corresponding to amino acids 200 to 456 of SEQ ID NO:4 or 16, except that Ser at the position corresponding to amino acid 351 has been substituted by Ala;(h) L.sub.1-199 Fc wherein L.sub.1-199 is the LBP sequence of amino acids corresponding to amino acids 1 to 199 of SEQ ID NO:2, 8 or 12, and an Fc portion of an immunoglobulin;(i) LBP that is the amino acid sequence corresponding to amino acids 1 to 456 of SEQ ID NO:2 or 12;(j) B.sub.(S351->A) is the BPI sequence corresponding to amino acids 1 to 456 of SEQ ID NO:4 or 16, except that Ser at the position corresponding to amino acid 351 has been substituted by Ala;(k) B.sub.(DS200->DP) is the BPI sequence corresponding to amino acids 1 to 456 of SEQ ID NO:4 or 16, except that Ser at the position corresponding to amino acid 201 has been substituted by Pro;(l) B.sub.1-199 L.sub.200-456 wherein B.sub.1-199 is the BPI sequence of amino acids corresponding to amino acids 1 to 199 of SEQ ID NO:4 and L.sub.200-456 is the LBP sequence of amino acids corresponding to amino acids 200-456 of SEQ ID NO:2 or 8; and(m) L.sub.1-199 is the LBP sequence corresponding to amino acids 1-199 of SEQ ID NO: 2, 8, or 12.