Patent ID: 6429003
Filing Date: 2002-08-06
Classification: C12N

Abstract:
An isolated nucleic acid fragment encoding an enzymatically active bacterial periplasmic nitrate reductase subunit, wherein said enzymatically active bacterial periplasmic nitrate reductase comprises NapA, NapB, NapC subunits selected from the group consisting of:(a) an isolated nucleic acid fragment encoding the amino acid sequence selected from the group consisting of SEQ ID NO:10, SEQ ID NO:12 and SEQ ID NO:14; (b) an isolated nucleic acid fragment having at least 90% identity with the nucleic acid sequence selected from the group consisting of SEQ ID NO:9, SEQ ID NO:11, and SEQ ID NO:13; and (c) an isolated nucleic acid fragment that hybridizes with SEQ ID NO:9, SEQ ID NO:11, and SEQ ID NO:13 under the following hybridization conditions: 0.1Ã—SSC, 0.1% SDS, 65Â° C.; or an isolated nucleic acid fragment that is complementary to (a), (b), or (c).