Patent ID: 6458600
Filing Date: 2002-10-01
Classification: B01J,C12Q,G01N

Abstract:
A method for producing a structure with electrically addressable immobilized molecules comprising the steps of:providing a chamber; providing in the chamber a non-reactive support having a plurality of electrically isolated electrodes, each having a conductive reactive surface, reactive with a compound containing one of a thiol group and a selenol group; selecting a first at least one electrically isolated electrode of said plurality of electrically isolated electrodes for immobilizing receptor molecules on the reactive surface thereof; introducing into the chamber an electrolyte solution containing a first set of receptor molecules comprised of a first compound containing one of a thiol group and a selenol group to be immobilized on the reactive surface of said first at least one selected electrically isolated electrode of said plurality of electrically isolated electrodes; contacting the first at least one selected electrically isolated electrode and the reactive surface thereof with the introduced electrolyte solution; electrically addressing the plurality of electrodes to apply an adsorption potential to said first at least one selected electrically isolated electrode while applying a desorption potential to the non-selected electrically isolated electrodes of said plurality of electrically isolated electrodes; applying an adsorption potential to said first at least one selected electrically isolated electrode and applying a desorption potential to the non-selected electrically isolated electrodes of said plurality of electrically isolated electrodes; chemically binding the first set of receptor molecules of the first compound in a monolayer on the reactive surface of the first at least one selected electrically isolated electrode to immobilize the first set of receptor molecules thereon; and inhibiting chemical binding of the first set of receptor molecules of the first compound on the non-selected electrodes of the plurality of electrodes and the reactive surfaces thereof thereby preventing the first set of receptor molecules of the first compound from being immobilized on the said non-selected electrodes of the plurality of electrodes.