Patent ID: 6346394
Filing Date: 2002-02-12
Classification: C12N,C12P,C12Y,Y10S

Abstract:
An isolated DNA molecule encoding a thermostable enzyme having the following physico-chemical properties:(1) Action Releasing trehalose from non-reducing saccharides having a trehalose structure as an end unit and a degree of glucose polymerization of at least 3, but not substantially acting on maltooligosaccharides having a degree of glucose polymerization of at least 3; (2) Molecular weight About 54,000-64,000 daltons on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE); and (3) Thermostability Substantially not inactivated even when incubated in an aqueous solution (pH 7.0) at 85Â° C. for 60 min, wherein said DNA molecule has a base sequence selected from the group consisting of SEQ ID NO:2 and a sequence similar to SEQ ID NO:2 where one or more bases in SEQ ID NO:2 is replaced with a different base without altering the corresponding amino acid sequence of SEQ ID NO:1.