Patent ID: 8815583
Filing Date: 2014-08-26
Classification: C12N,C12P

Abstract:
1. A CHO KI cell transfected with an expression vector for expressing peptidylglycine alpha-amidating monooxygenase, wherein said expression vector has a coding region encoding peptidylglycine alpha-amidating monooxygenase, wherein the coding region is operably linked to a control region including a ribosome binding site, a promoter, and an SV40 enhancer upstream of said promoter, wherein the promoter is an inducible human metallothionein IIa promoter and is located immediately upstream of a start site of the coding region, wherein the SV40 enhancer is located immediately upstream of the inducible human metallothionein IIa promoter, wherein the peptidylglycine alpha-amidating monooxygenase consists of a signal peptide at amino acid residues 1 to 25, a pro-region at amino acid residues 26 to 41, and a mature peptidylglycine alpha-amidating monooxygenase at amino acid residues 42 to 715 as set forth in SEQ ID NO: 2, wherein the CHO K1 cell is capable of expressing a soluble peptidylglycine alpha-amidating monooxygenase.