Patent ID: 7422861
Filing Date: 2008-09-09
Classification: A61K,C07K,C12N,Y02P

Abstract:
1. A method of identifying peptoids which are effective in transfecting a cell with an oligonucleotide, the method comprising (i) providing a library of peptoids in an array of physically separated compartments, said peptoids having a plurality of different sequences and having the general formula I: where R each R wherein at least one group R R X is selected from hydroxy, alkoxy, amino, guanidino, amidino, alkylamino, alkylthio, halogen, nitro, cyano, keto, aldehyde, carboxylic acid, carboxylic ester, carboxylic amide, sulfonic acid and sulfonic ester; at least one of R R m is an integer selected from 2 to about 50, wherein the sequences of individual peptoids in the library are unidentified; (ii) contacting a plurality of peptoids having unidentified sequences from the library provided in (i) with an oligonucleotide, to form a plurality of peptoid-oligonucleotide mixtures, wherein said oligonucleotide is between about 10 and 50 nucleotides in length, and is a fluorescently labelled or an antisense oligonucleotide, and wherein said contacting is performed in an array of physically separated compartments; (iii) contacting each said mixture with a cell in an array of physically separated compartments; (iv) screening each cell for transfection of the oligonucleotide, to identify transfected cells; and (v) identifying transfecting peptoids in mixtures contacted with transfected cells.