Patent ID: 7314631
Filing Date: 2008-01-01
Classification: A61K,C07K,C12N

Abstract:
1. An infectious chimeric parainfluenza virus (PIV) comprising a major nucleocapsid (N) protein, a nucleocapsid phosphoprotein (P), a large polymerase protein (L), and a partial or complete human parainfluenza virus 3 (HPIV3) background genome or antigenome combined with one or more heterologous gene(s) or genome segment(s) encoding a complete open reading frame or one or more antigenic determinant(s) selected from the group consisting of: an HN glycoprotein of HPIV 1, an HN glycoprotein of HPIV2, an F glycoprotein of HPIV1 and an F glycoprotein of HPIV2, said heterologous gene(s) or gene segment(s) being operably linked to regulatory sequences operable in said HPIV3 genome or antigenome, to form a chimeric parainfluenza virus (PIV) genome or antigenome; said partial or complete PIV background genome or antigenome comprising a polynucleotide encoding a wild-type L protein of the background PIV; said infectious chimeric PIV being attenuated for replication at least 10-fold in the respiratory tract of a primate host infected with said chimeric PIV.