Patent ID: 6451540
Filing Date: 2002-09-17
Classification: C07H,C12N,C12Y

Abstract:
A method comprising the steps of:a) contacting an enzymatic nucleic acid molecule with a sample under conditions suitable for said enzymatic nucleic acid molecule to catalyze a chemical reaction with a target molecule, said enzymatic nucleic acid molecule comprising one or more modified nucleotides, wherein said modified nucleotide has the formula: wherein,R1 is independently 2â€²-O-R3-thio-R3 or 2â€²-C-R3-thio-R3, wherein said R3 is independently a compound selected from a group consisting of alkyl, allyl, alkenyl, alkynyl, aryl, alkylaryl, carbocyclic aryl, heterocyclic aryl, amide and ester; X is independently a base or H; Y represents a phosphorus-containing group; and R2 represents H, a blocking group or a phosphorous-containing group; and b) assaying for said chemical reaction catalyzed by said enzymatic nucleic acid molecule.