Patent ID: 6184042
Filing Date: 2001-02-06
Classification: C07K,G01N,Y10S

Abstract:
A method for extending the concentration range at which an analyte can be detected in a sample liquid according to the principle of a sandwich assay comprising the step ofa) incubating the sample liquid in the presence of a solid phase with at least two receptors that are each capable of binding to the analyte wherein the first receptor is soluble and the second receptor (i) is bound to a solid phase or (ii) is capable of binding to a solid phase; andb) detecting the analyte by determining a label in the solid phase, the liquid phase, or both the solid phase and the liquid phase,wherein the first receptor is a labeled oligomer of a binding molecule selected from antibodies, antibody fragments, and mixtures thereof, and wherein all of said antibodies and antibody fragments bind to the same antigen, in an amount sufficient to reduce the Hook Effect.