Patent ID: 8435764
Filing Date: 2013-05-07
Classification: C12Q

Abstract:
1. A method for the detection of hepatitis B virus (HBV) variations, comprising, a) providing an HBV DNA molecule from a subject suffering from an HBV infection as a template, wherein the DNA molecule includes an HBV variation site; b) extending multiplex extension primers along the template to obtain extension products containing nucleotides at the variation sites; and c) analyzing the extension products by mass spectrometry (MS) to detect the HBV variations, wherein the DNA molecule is provided by conducting a 2-plex PCR, wherein each primer has at least 70% identity to one of the primers set forth in SEQ ID NOs: 1-4, and wherein the multiplex extension primers are extended by using a set of primers selected from the group consisting of (i) the primers having at least 70% identity to those as set forth in SEQ ID NOs: 5-16 in a 12-plex reaction, (ii) the primers having at least a 70% identity with those as set forth in SEQ ID NOs: 17-33 in a 17-plex reaction, (iii) the primers having at least a 70% identity with those as set forth in SEQ ID NOs: 34-50 as set forth in a 17-plex reaction, and (iv) the primers having at least a 70% identity with those as set forth in SEQ ID NOs: 51-64 in a 14-plex reaction.