Patent ID: 9012718
Filing Date: 2015-04-21
Classification: C12N

Abstract:
1. A nucleic acid sequence encoding a mutated delta-12 oleate desaturase enzyme (FAD2) from a plant of Brassica napus species, wherein said nucleic acid sequence comprises : a) nucleic acid sequence SEQ ID NO:22 in which at least one nucleotide of a codon representing the proline at position 259 of the polypeptide encoded by nucleic acid sequence SEQ ID NO:22 is mutated and produces a non-conservative substitution of said proline at position 259 of the polypeptide encoded by nucleic acid sequence SEQ ID NO:22, or b) a nucleic acid sequence having at least 90% identity with the sequence defined in a) and which comprises a mutation producing a non-conservative substitution at an amino acid position corresponding i) to proline at position 259 of the polypeptide encoded by nucleic acid sequence SEQ ID NO:22, and which does not encode a functional FAD2; or c) the complementary sequences of the sequence defined in a) or b).