Patent ID: 9005953
Filing Date: 2015-04-14
Classification: C12N,C12P,Y02E

Abstract:
1. A recombinant microorganism, comprising an acetoacetyl-CoA synthase gene encoding a protein having the amino acid sequence of SEQ ID NO: 1, and a group of genes from Clostridium acetobutvlicum that are involved in butanol biosynthesis that enables synthesis of butanol from acetoacetyl-CoA, wherein the genes are introduced into a host microorganism, wherein the group of genes involved in butanol biosynthesis consists of the β-hydroxybutyryl-CoA dehydrogenase gene, the 3-hydroxybutyryl-CoA dehydratase gene, the butyryl-CoA dehydrogenase gene, the butyraldehyde dehydrogenase gene, and the butanol dehydrogenase gene, wherein the acetoacetyl-CoA synthase gene is a gene from an actinomycete of the genus Streptomyces , and wherein the host microorganism is Escherichia coli.