Patent ID: 8597914
Filing Date: 2013-12-03
Classification: C12M,C12N,C12P

Abstract:
1. A method of producing a fermentable sugar using a photobioreactor, the method comprising: (A) inoculating a photobioreactor with a transgenic cyanobacteria engineered to accumulate at least one fermentable sugar selected from the group consisting of sucrose and trehalose, wherein the transgenic cyanobacteria comprises an artificial DNA construct comprising wherein (1) the second polypeptide comprises SEQ ID NO: 4 or a polypeptide that is 95% identical to SEQ ID NO: 4 having sucrose phosphate synthase (SPS) activity and the third polypeptide comprises SEQ ID NO: 6 or a polypeptide that is 95% identical to SEQ ID NO: 6 having sucrose phosphate phosphatase (SPP) activity; or (2) the second polypeptide comprises SEQ ID NO: 77 or a polypeptide that is 95% identical to SEQ ID NO: 77 having trehalose phosphate synthase (TPS) activity and the third polypeptide comprises SEQ ID NO: 79 or a polypeptide that is 95% identical to SEQ ID NO: 79 having trehalose phosphate phosphatase (TPP) activity; and (B) cultivating the transgenic cyanobacteria in or on the photobioreactor; and (C) isolating accumulated fermentable sugar.