Patent ID: 6406869
Filing Date: 2002-06-18
Classification: C12Q,G01N

Abstract:
A method for measuring protein tyrosine kinase activity in a biological sample, said method comprising the steps:(a) coating a solid phase with a first anti-phosphotyrosine antibody; (b) contacting a biological sample with said solid phase coated with said first anti-phosphotyrosine antibody so that phosphorylated tyrosine-containing molecules in the biological sample are bound by the antibody to the solid phase; (c) contacting bound phosphorylated tyrosine-containing molecules with a second anti-phosphotyrosine antibody; (d) removing unbound second anti-phosphotyrosine antibody; and (e) determining the level of tyrosine kinase activity in said biological sample by measuring the amount of said second anti-phosphotyrosine.