Patent ID: 6319540
Filing Date: 2001-11-20
Classification: A61B,C12Q

Abstract:
A method of immobilizing a boronic acid moiety in a solid support, the method comprising:covalently attaching the boronic acid moiety to a solid support comprising a polymer matrix, wherein the boronic acid moiety comprises a compound of the formula: ##STR2##wherein D.sup.1 is a dye selected from the group consisting of fluorescent dyes, luminescent dyes and colorimetric dyes;R.sup.1, R.sup.3 and R.sup.4 are each independently substituents which can alter the electronic properties of the groups to which they are attached or are functional groups which can form covalent linkages to the polymer matrix;each R.sup.2 is a member independently selected from the group consisting of hydrogen and C.sub.1 -C.sub.4 alkyl;each L.sup.1 and L.sup.2 is a linking group having from zero to four contiguous atoms selected from the group consisting of carbon, oxygen, nitrogen, sulfur and phosphorus;Z is a heteroatom selected from the group consisting of nitrogen, sulfur, oxygen and phosphorus;x is an integer from zero to four; and wherein the boronic acid moiety is attached to the polymer matrix by a covalent linkage to R.sup.1.