Patent ID: 6790634
Filing Date: 2004-09-14
Classification: G01N,Y10T

Abstract:
A method for identifying an agent which inhibits damage to cardiac and skeletal muscle comprising:(a) combining adenosine triphosphate and a test agent with purified myosin, myosin light chain 1 (MLC1) or isoform thereof, or myofilament or skinned muscle fiber in the presence of an enzyme that modulates phosphorylation of MLC1; and (b) monitoring phosphorylation of MLC1 in the purified myosin, myosin light chain 1 (MLC1) or isoform thereof, or myofilament or skinned muscle fiber in the presence and absence of the test agent, wherein an increase in MLC1 phosphorylation in the purified myosin, myosin light chain 1 (MLC1) or isoform thereof, or myofilament or skinned muscle fiber in the presence of the test agent is indicative of the test agent inhibiting damage to cardiac and skeletal muscle.