Patent ID: 6498025
Filing Date: 2002-12-24
Classification: C12N,C12Q

Abstract:
A method for synthesizing a complementary DNA copy of an RNA template molecule which method comprises:(a) providing at least one polynucleotide molecule which functions as a primer molecule for at least one reverse transcriptase enzyme and which anneals in vivo to an RNA template molecule at a position other than a naturally-occurring retroviral primer binding site, wherein said polynucleotide molecule comprises a stem loop structure recognized by said reverse transcriptase enzyme; (b) providing at least one reverse transcriptase enzyme which initiates DNA synthesis in vivo using the polynucleotide molecule as a primer; (c) introducing the polynucleotide molecule into a viable target cell in the presence of the reverse transcriptase enzyme; and (d) incubating the target cell under conditions which permit the synthesis of a DNA molecule complementary to the RNA template molecule.