Patent ID: 8541207
Filing Date: 2013-09-24
Classification: C12Q

Abstract:
1. A method of sequencing nucleic acid comprising deaminated cytosine, said method comprising: providing a sample comprising a template nucleic acid; generating a complementary copy of said template nucleic acid, said generating being directed by an oligonucleotide primer using a nucleic acid polymerase in the presence of a bisulfite-resistant cytosine analog, wherein said generating produces a complementary copy of said template nucleic acid such that cytosine residues in said complementary copy are bisulfite-resistant; subjecting said template nucleic acid and said complementary copy to bisulfite treatment to convert unmethylated cytosine residues in said template nucleic acid into uracil residues, resulting in a bisulfite-converted template nucleic acid and a non-converted complementary copy; and determining the nucleotide sequence of said bisulfite-converted template nucleic acid and said non-converted complementary copy.