Patent ID: 7655794
Filing Date: 2010-02-02
Classification: C12N

Abstract:
1. In a method for the separation and/or purification of a nucleic acid from cells, comprising: a) generating an aqueous solution containing the nucleic acid by lysing said cells with a lysis solution; b) contacting said aqueous solution containing the nucleic acid with an anion exchanger bound to a solid support matrix for the nucleic acid to bind; c) eluting the nucleic acid from said anion exchanger with an aqueous mobile phase comprising a basic nucleic acid elution solution at a pH of about 9 to a pH of about 13; and d) desalting said eluted nucleic acid; wherein the improvement comprises providing said anion exchanger in a packed column, wherein said column is packed with a salt solution containing an antimicrobial agent, further wherein the salt concentration in said salt solution is similar to the salt concentration of said aqueous solution in said contacting step such that said column does not need equilibration prior to said contacting step; and provided that the nucleic acid elution solution is made basic by the addition of a sufficient quantity of a compound selected from the group consisting of potassium carbonate, arginine and guanidine carbonate.