Patent ID: 6306600
Filing Date: 2001-10-23
Classification: C07K,C12N

Abstract:
A method of assaying the positive or negative regulatory function of a transcriptional or translational control sequence which controls or regulates the expression of a coding sequence in a host cell with a transient fluorescent reporter protein, comprising the steps of:a) transfecting cells with an expression vector comprising a DNA sequence that codes for a fluorescent fusion protein comprising a Green Fluorescent Protein (GFP) and a PEST sequence, wherein said fusion protein has a half life of no more than about ten hours, and wherein said expression vector contains said transcriptional or translational control sequence that may affect the expression of said fluorescent fusion protein;b) transfecting cells with a control expression vector comprising a DNA sequence that codes for said fluorescent fusion protein, wherein said control expression vector does not contain said transcriptional or translational control sequence; andc) detecting a presence, absence or amount of fluorescence in said cells, wherein an increase or decrease in fluorescence in cells transfected with the vector containing the control sequence compared to cells transfected with the vector not containing the control sequence indicates a positive or negative regulatory function, respectively for said transcriptional or translational control sequence.