Patent ID: 7226763
Filing Date: 2007-06-05
Classification: C12N,C12R

Abstract:
1. A fermentation process for the preparation of an L-amino acid, comprising: (a) fermenting in a medium corynebacteria producing the L-amino acid, wherein a nucleotide sequence which encodes a protein kinase D (pknD) polypeptide is amplified in the corynebacteria, and (b) enriching the L-amino acid in the medium or in the cells of the corynebacteria wherein the nucleotide sequence is selected from the group consisting of: (a) a nucleotide sequence shown in SEQ ID NO: 1, (b) a polynucleotide which is at least 90% identical to the polynucleotide of SEQ ID NO: 1, wherein said polynucleotide codes for a polypeptide having protein kinase D activity, (c) a polynucleotide coding for a polypeptide comprising the amino acid sequence of SEQ ID NO: 2, and (d) a sequence which hybridizes with SEQ ID NO: 1, wherein hybridization comprises washing in 0.5×SSC at a temperature of 68° C., and which encodes a protein with protein kinase D activity.