Patent ID: 7960537
Filing Date: 2011-06-14
Classification: C12Q,Y10T

Abstract:
1. A composition comprising a plurality of chemically synthesized oligonucleotide tag complements, each oligonucleotide having a 5′ residue and a 3′ residue and being characterized in that: (a) each oligonucleotide is free of either cytosine or guanosine residues; (b) any two cytosine or guanosine residues are separated by at most 6 non-cytosine or non-guanosine residues, respectively; (c) the length of each oligonucleotide differs by no more than five bases from the average length of all oligonucleotides in the composition; and (d) when each oligonucleotide tag complement is exposed to hybridization conditions comprising 0.2M NaCl, 0.1M Tris, 0.08% Triton X-100, pH 8.0 at 37° C., the maximum degree of hybridization between the tag complement and a tag not fully complementary to the tag complement does not exceed 25% of the degree of hybridization between the tag complement and its fully complementary tag.