Patent ID: 9012614
Filing Date: 2015-04-21
Classification: C07H,C12N,C12Q,G01N,Y10T

Abstract:
1. A method for rapid nucleic acid purification, wherein a nucleic acid is isolated from a biological sample, the method comprising the following steps in order: 1) obtaining a cell lysate by mixing the biological sample with a cell lysis buffer comprising a chaotropic salt and a sarcosinate, said cell lysate including a nucleic acid originated from the biological sample, and immersing a silica membrane into the cell lysate so that the nucleic acid is adsorbed on the silica membrane; 2) immersing the silica membrane of step 1) into a mobile phase buffer for a sufficient time for the nucleic acid to migrate on the silica membrane; and 3) immersing the silica membrane of step 2) into a nucleic acid washing buffer to elute the nucleic acid or performing at least one technique selected from PCR, RT-PCR, real-time PCR, and real-time RT-PCR directly on the nucleic acid bound onto the silica membrane.