Patent ID: 6255104
Filing Date: 2001-07-03
Classification: A61K,C07K,C12N,Y02A

Abstract:
A composition comprising(1) a recombinant poliovirus vector, wherein the vector comprises an RNA polymerase promoter operably linked to a poliovirus nucleic acid sequence that has been altered by(a) deleting the CelII-SnaBI sequence segment fragment, wherein said fragment encodes the coat proteins, VP1, VP2, VP3, and VP4, and(b) inserting therein a heterologous nucleic acid sequence encoding a protein, wherein the heterologous nucleic acid sequence is flanked on both sides by internal ribosomal entry sites (IRES), said IRES allowing for the expression of the encoded protein and for re-initiating expression of subsequent proteins of the altered poliovirus nucleic acid sequence; and(2) at least one helper virus for complementation of the coat proteins encoded by the deleted sequence segment fragment.