Patent ID: 6485954
Filing Date: 2002-11-26
Classification: A23K,A23L,C12G,C12N,C12Y

Abstract:
An isolated polypeptide exhibiting galactanase activity selected from the group consisting of:(a) a polypeptide encoded by the galactanase enzyme encoding part of the DNA sequence cloned into plasmid pYES 2.0 present in Saccharomyces cerevisiae DSM 9983; (b) a polypeptide comprising an amino acid sequence of amino acid residues 19-350 of SEQ ID NO: 2 or a fragment thereof that exhibits galactanase activity; and (c) a polypeptide which has an amino acid sequence that is at least 80% homologous with the polypeptide of amino acid residues 19-350 of SEQ ID NO: 2, wherein the homology is determined using GAP with a GAP creation penalty of 3.0 and a GAP extension penalty of 0.1; and (d) a polypeptide encoded by a DNA sequence that hybridizes with SEQ ID NO: 1 under high stringency conditions, wherein the conditions are defined as prehybridization in a solution of 5Ã—SSC, 5Ã—Denhardt's solution, 0.5% SDS and 100 &mgr;g/ml of denatured sonicated salmon sperm DNA, followed by hybridization in the same solution containing a concentration of 10 ng/ml of a random-primed 32P-dCTP-labeled probe for 12 hours at a temperature of about 45Â° C. followed by washing for 30 minutes in 2Ã—SSC, 0.5% SDS at a temperature of at least 70Â° C.