Patent ID: 6238922
Filing Date: 2001-05-29
Classification: C12N

Abstract:
A method for the in vitro proliferation of a neural stem cell culture comprising the steps of:(a) obtaining dissociated neural tissue in cell suspension, the suspension containing one or more multipotent neural stem cells capable of producing progeny that are capable of differentiating into neurons and glia;(b) culturing the cell suspension in a culture medium containing at least one proliferation-inducing growth factor to proliferate the neural stem cells in (a) to generate a neural stem cell culture comprising aggregated neural stem cells; and(c) passaging the cell culture in (b) by treating the culture with an amount of a collagenase preparation effective to dissociate the aggregated neural stem cells in the culture and passing the cell culture to a culture medium containing at least one proliferation-inducing growth factor to further proliferate the neural stem cell culture.