Patent ID: 8343724
Filing Date: 2013-01-01
Classification: C12Q

Abstract:
1. A method for determining a mix rate of genetically modified wheat in a test sample, comprising: performing quantitative PCR using, as templates, a circular DNA comprising nucleotide sequence of SEQ ID NO:7 capable of being amplified by PCR using a primer pair comprising a first primer comprising the nucleotide sequence of SEQ ID NO:20 and a second primer comprising the nucleotide sequence of SEQ ID NO:21 and DNA extracted from the test sample, preparing a calibration curve for determining the number of molecules of template DNA, using the results of the quantitative PCR for the circular DNA, determining the number of molecules having a partial sequence of an endogenous wheat DNA sequence and the number of molecules having a partial sequence of a DNA sequence present specifically in at least one kind of genetically modified wheat contained in the test sample, using the calibration curve and the results of the quantitative POR for the test sample, and determining a ratio A obtained by dividing the number of molecules having a partial sequence of a DNA sequence present specifically in the genetically modified wheat by the number of molecules having a partial sequence of an endogenous wheat DNA sequence.