Patent ID: 6261774
Filing Date: 2001-07-17
Classification: A61K,B82Y,C07H,C07K,C12N,C12Q,C40B,F02B,G01N

Abstract:
A method for identifying nucleic acid ligands of a target molecule from a candidate mixture comprised of single stranded nucleic acids each having a region of randomized sequence and a region of fixed sequence, said method comprising:a) preparing a candidate mixture of single-stranded nucleic acids wherein each nucleic acid member of said candidate mixture comprises a region of randomized sequence and a region of fixed sequence;b) annealing oligonucleotides to the fixed sequences that are complementary to said fixed sequences to form duplex regions, wherein said fixed sequences of said duplex regions are unavailable for binding to said target;c) contacting said candidate mixture with said target molecule;d) partitioning the nucleic acids having an increased affinity to the target molecule relative to the candidate mixture from the remainder of the candidate mixture; ande) amplifying the increased affinity nucleic acids, in vitro, to yield a ligand-enriched mixture of nucleic acids whereby nucleic acid ligands of the target molecule are identified.