Patent ID: 6410239
Filing Date: 2002-06-25
Classification: B01J,B82Y,C12N,C40B,G01N,G16B

Abstract:
A method of detecting one or more protein-protein interactions comprising(a) recombinantly expressing within a population of host cells (i) a first population of first fusion proteins, each said first fusion protein comprising a first protein sequence and a DNA binding domain in which the DNA binding domain is the same in each said first fusion protein, and in which said first population of first fusion proteins has a complexity of at least 100; and (ii) a second population of second fusion proteins, each said second fusion protein comprising a second protein sequence and a transcriptional regulatory domain of a transcriptional regulator, in which the transcriptional regulatory domain is the same in each said second fusion protein, such that a first fusion protein is co-expressed with a second fusion protein in host cells, and wherein said host cells contain at least one nucleotide sequence operably linked to a promoter driven by one or more DNA binding sites recognized by said DNA binding domain such that interaction of a first fusion protein with a second fusion protein results in regulation of transcription of said at least one nucleotide sequence by said regulatory domain, and in which said second population of second fusion proteins has a complexity of at least 100; wherein a host cell comprises a first fusion protein and a second fusion protein, and the number of host cells in the population is sufficiently large to provide confidence at the level of 50% or greater that every pair wise combination of a first fusion protein and a second fusion protein is represented in the population; and (b) detecting said regulation of transcription of said at least one nucleotide sequence, thereby detecting cells in which an interaction between a first fusion protein and a second fusion protein has occurred.