Patent ID: 8945835
Filing Date: 2015-02-03
Classification: C12Q

Abstract:
1. A method for sequencing, the method comprising: (a) providing a single-stranded polynucleotide immobilized on a planar support, wherein the single-stranded polynucleotide comprises: (b) hybridizing a first primer to the first region of known sequence in the single-stranded polynucleotide immobilized on the planar support; (c) extending the first primer to form a first extended primer hybridized to the single-stranded polynucleotide, wherein the first extended primer comprises a fluorescent label; (d) detecting fluorescence emitted from the fluorescent label in the first extended primer while the first extended primer is hybridized to the single-stranded polynucleotide immobilized on the planar support; (e) denaturing the first extended primer from the single-stranded polynucleotide; (f) hybridizing a second primer to the second region of known sequence in the single-stranded polynucleotide immobilized on the planar support, wherein the second primer is hybridized to the single-stranded polynucleotide after denaturing the first extended primer from the single-stranded polynucleotide; (g) extending the second primer to form a second extended primer hybridized to the single-stranded polynucleotide immobilized on the planar support, wherein the second extended primer comprises a fluorescent label; and (h) detecting fluorescence emitted from the fluorescent label in the second extended primer while the second extended primer is hybridized to the single-stranded polynucleotide.