Patent ID: 8759046
Filing Date: 2014-06-24
Classification: C07K,C12N,C12P

Abstract:
1. A method of producing a prenyl alcohol(s) selected from the group consisting of farnesol, nerolidol and geranylgeraniol, comprising the steps of: culturing a mutant cell into which a fusion gene of farnesyl diphosphate synthase gene and geranylgeranyl diphosphate synthase gene has been introduced to produce a fusion protein having both farnesyl diphosphate synthase activity and geranylgeranyl diphosphate synthase activity; wherein the mutant cell is derived from a mutant strain of recovering the prenyl alcohol(s) selected from the group consisting of farnesol, nerolidol and geranylgeraniol from the resultant culture; wherein the farnesol diphosphate (FPP) synthase gene encodes a polypeptide having the amino acid sequence set forth in SEQ ID NO:2 or 4 or a polypeptide having FPP synthase activity and an amino acid sequence derived from the amino acid sequence set forth in SEQ ID NO:2 or 4 by deletion, substitution, or addition of from one to ten amino acids; and wherein the geranylgeranyl diphosphate (GGPP) synthase gene encoding a polypeptide having the amino acid sequence set forth in SEQ ID NO:6 or a polypeptide having GGPP synthase activity and an amino acid sequence derived from the amino acid sequence set forth in SEQ ID NO:6 by deletion, substitution, or addition of from one to ten amino acids.