Patent ID: 7241606
Filing Date: 2007-07-10
Classification: A21D,A23C,A23G,A23L,A61K,A61Q,C07H,C12N,C12P,C12Y

Abstract:
1. A purified α-isomatosylglucosaccharide-forming enzyme which forms a saccharide having a glucose polymerization degree of at least three and having both the α-1,6 glucosidic linkage as a linkage at the non-reducing end and the α-1,4 glucosidic linkage other than the linkage at the non-reducing end, by catalyzing α-glucosyl-transfer from a saccharide having a glucose polymerization degree of at least two and having an α-glucosidic linkage at the non-reducing end; which enzyme comprises an amino acid sequence of SEQ ID NO:1, SEQ ID NO:11 or SEQ ID NO:18 as a partial amino acid sequence; said enzyme being incapable of forming dextran, said enzyme being inhibited by EDTA, and said enzyme activated by Ca 2+ and Mn 2+ ; thermal stability of said enzyme being improved by Ca 2+ ; and said enzyme having the following physicochemical properties: a. Molecular weight b. Isoelectric point (pI) c. Optimum temperature d. Optimum pH e. Thermal stability f. pH stability Stable at pHs of about 5.0 to about 9.0 when incubated at 4° C. for 24 hours.