Patent ID: 6991924
Filing Date: 2006-01-31
Classification: C12N,C12P

Abstract:
1. A DNA coding for a mutant of a large subunit of a carbamoylphosphate synthetase, wherein said mutant has an amino acid sequence of a large subunit of carbamoylphosphate synthetase as defined in the following (A) or (B), whereby amino acid residues corresponding to positions 947 to 951 of SEQ ID NO:20 are replaced with any one of amino acid sequences of SEQ ID NOs:1 to 9, and is desensitized to feedback inhibition by uridine 5′-monophosphate: (A) a protein having the amino acid sequence defined in SEQ ID NO:20; or (B) a protein that is encoded by a DNA which hybridizes with a DNA having the nucleotide sequence defined in SEQ ID NO:19 under stringent conditions, and has a carbamoylphosphate synthetase activity, wherein said stringent conditions entail a temperature of 60° C., a salt concentration of 0.1×SSC, and 0.1% SDS.