Patent ID: 6133044
Filing Date: 2000-10-17
Classification: G01N,Y10T

Abstract:
A method of creating a droplet from a jet of a flow cytometer comprising the steps of:a. establishing a nozzle volume defined by a nozzle body;b. introducing a flow of sheath fluid into said nozzle volume;c. introducing a flow of a substance within said sheath fluid in said nozzle volume;d. establishing a substantially isolated unidirectional coupling with said nozzle volume which couples an oscillator to said nozzle volume through use of a directional isolator situated between said nozzle body and said oscillator;e. creating a substantially isolated unidirectional oscillation within said nozzle volume using an alternating voltage with an amplitude of less than one hundred millivolts for said oscillator;f. allowing said sheath fluid to exit from said nozzle volume; andg. forming at least one substance entraining droplet from said sheath fluid after allowing said sheath fluid to exit from said nozzle volume.