Patent ID: 7704509
Filing Date: 2010-04-27
Classification: A61K,A61P,C07K,C12N

Abstract:
1. A method for producing an infectious, self-replicating, recombinant human parainfluenza virus type 1 (HPIV 1) from one or more isolated polynucleotide molecules encoding said HPIV1, comprising: coexpressing in a cell or cell-free system one or more expression vector(s) comprising a polynucleotide molecule that encodes a partial or complete, recombinant HPIV 1 genome or antigenome and one or more polynucleotide molecules encoding PIV N, P and L proteins, thereby producing an infectious HPIV 1, said recombinant HPIV1 genome or antigenome including at least one mutation selected from the group consisting of: a mutation in the L protein Y942 to an amino acid selected from the group consisting of F, C, N, D, W, S, Q, G, T, V, M, L and A;