Patent ID: 6593086
Filing Date: 2003-07-15
Classification: C12P,C12Q,Y10T

Abstract:
A method for detection of a target nucleic acid within a cell comprising the steps of:(a) embedding the cell to be analyzed for the presence of the target nucleic acid within a matrix; (b) adding a circularizable Amp-probe-2 having 3â€² and 5â€² regions that are complementary to adjacent but noncontiguous sequences in the target nucleic acid, said 3â€² and 5â€² regions separated by a linker region that is neither complementary nor hybridizable to a nucleotide sequence in the target nucleic acid such that a complex is formed comprising the target nucleic acid and Amp-probe-2; (c) ligating the 3â€² and 5â€² ends of said circularizable Amp-probe-2 with a ligating agent that joins nucleotide sequences such that a circular Amp-probe-2 is formed; (d) washing said complex; and (e) detecting said complex, wherein the detection thereof indicates the presence of the target nucleic acid in the embedded cell.