Patent ID: 7754447
Filing Date: 2010-07-13
Classification: C12N,C12P

Abstract:
1. A method for determining the growth rate of E. coli host cells that results in partitioning of a recombinant protein produced by the host cells between the periplasm of the host cells and the supernatant of the culture medium in which the cells are grown that is most suited to the primary recovery of the recombinant protein, wherein expression of the recombinant protein by said cells is under the control of an inducible system, which method comprises: a) providing an b) changing the growth rate of the c) inducing expression of the recombinant protein wherein steps (b) and (c) are performed in any order or simultaneously; and subsequently d) determining the yield of recombinant protein in the supernatant of the culture medium and the e) performing steps (b) and (d), and optionally steps (a) and (c), using a growth rate different from the growth rate initially used in step (b) f) comparing the yield determined in step (d) with the yield determined in step (e); and g) selecting a growth rate from the comparison made in step (f), the selected growth rate being in the range of 0.0005 to 0.0092/h, the selected growth rate being most suited to the primary recovery of the recombinant protein from the supernatant.