Patent ID: 8097447
Filing Date: 2012-01-17
Classification: C12N,C12Q

Abstract:
1. A solid nutrient media composition having alkaline pH, useful for isolating and identifying alkaliphilic microorganisms in pure form, wherein the media composition per liter of distilled water consists essentially of 5-15 g of carbon source, 2.5 to 10 g of peptone, 2.5 to 10 g of yeast extract, 0.5 to 1.5 g of dipotassium hydrogen phosphate; 0.1 to 0.5 g of magnesium sulphate heptahydrate, 30 μl to 4 ml of super saturated solution of sodium hydroxide, 5 to 20 g of potassium chloride and 10 to 30 g of κ-carrageenan, wherein the said media has a pH of greater than 12.5 and a gel strength of greater than 200 g/cm 2 .