Patent ID: 6080540
Filing Date: 2000-06-27
Classification: C07K,C12N

Abstract:
A method of identifying a chemical agent which inhibits a mammalian gene which, when expressed in a genetically altered microorganism, modifies a phenotypic alteration associated with a genetic alteration in the microorganism, comprising the steps of:a) expressing the mammalian gene in a genetically altered microorganism, thereby modifying the phenotypic alteration associated with the genetic alteration;b) contacting the genetically altered microorganism of step (a) with a chemical agent to be assayed, under conditions appropriate for phenotypic assay; andc) determining whether the phenotypic alteration associated with the genetic alteration modified in step (a) is reversed, wherein reversal of the phenotypic alteration is indicative of a chemical agent which inhibits the mammalian gene.