Patent ID: 6077935
Filing Date: 2000-06-20
Classification: A61K,C07K,C12N,C12Q

Abstract:
An unglycosylated HTLV-III protein, said protein being essentially free of other proteins, and having the following amino acid subsequence of the HTLV-III envelope protein, from amino to carboxy terminus:ValTrpLysGluAla - ThrThrThrLeuPheCysAlaSerAspAlaLysAlaTyrAspThrGlu - ValHisAsnValTrpAlaThrHisAlaCysValProThrAspProAsn - ProGlnGluValValLeuValAsnValThrGluAsnPheAsnMETTrp - LysAsnAspMETValGluGlnMETHisGluAspIleIleSerLeuTrp - AspGlnSerLeuLysProCysValLysLeuThrProLeuCysValSer - LeuLysCysThrAspLeuLysAsnAspThrAsnThrAsnSerSerSer - GlyArgMETIleMETGluLysGlyGluIleLysAsnCysSerPheAsn - IleSerThrSerIleArgGlyLysValGlnLysGluTyrAlaPhePhe - TyrLysLeuAspIleIleProIleAspAsnAspThrThrSerTyrThr - LeuThrSerCysAsnThrSerValIleThrGlnAlaCysProLysVal - SerPheGluProIleProIleHisTyrCysAlaProAlaGlyPheAla - IleLeuLysCysAsnAsnLysThrPheAsnGlyThrGlyProCysThr - AsnValSerThrValGlnCysThrHisGlyIleArgProValValSer - ThrGlnLeuLeuLeuAsnGlySerLeuAlaGluGluGluValValIle - ArgSerValAsnPheThrAspAsnAlaLysThrIleIleValGlnLeu - AsnThrSerValGluIleAsnCysThrArgProAsnAsnAsnThrArg - LysLysIleArgIleGlnArgGlyProGlyArgAlaPheValThrIle - GlyLysIleGlyAsnMETArgGlnAlaHisCysAsnIleSerArgAla - LysTrpAsnAlaThrLeuLysGlnIleAlaSerLysLeuArgGluGln - PheGlyAsnAsnLysThrIleIlePheLysGlnSerSerGlyGlyAsp - ProGluIleValThrHisSerPheAsnCysGlyGlyGluPhePheTyr - CysAsnSerThrGlnLeuPheAsnSerThrTrpPheAsnSerThrTrp - SerThrGluGlySerAsnAsnThrGluGlySerAspThrIleThrLeu - ProCysArgIleLysGlnPheIleAsnMETTrpGlnGluValGlyLys - AlaMETTyrAlaProProIleSerGlyGlnIleArgCysSerSerAsn - IleThrGlyLeuLeuLeuThrArgAspGlyGlyAsnAsnAsnAsnGly - SerGluIlePheArgProGlyGlyGlyAspMETArgAspAsnTrpArg - SerGluLeuTyrLysTyrLysValValLysIleGluProLeuGlyVal - AlaProThrLysAlaLysArgArgValValGlnArgGluLysArgAla - ValGlyIleGlyAlaLeuPheLeuGlyPheLeuGlyAlaAlaGlySer - ThrMETGlyAlaAlaSerMETThrLeuThrValGlnAlaArgGlnLeu - LeuSerGlyIleValGlnGlnGlnAsnAsnLeuLeuArgAlaIleGlu - AlaGlnGlnHisLeuLeuGlnLeuThrValTrpGlyIleLysGlnLeu - GlnAlaArgIleLeuAlaValGluArgTyrLeuLysAspGlnGlnLeu - LeuGlyIleTrpGlyCysSerGlyLysLeuIleCysThrThrAlaVal - ProTrpAsnAlaSerTrpSerAsnLysSerLeuGluGlnIleTrpAsn - HisThrThrTrpMETGluTrpAspArgGluIleAsnAsnTyrThrSer or a subsequence thereof, said protein containing at least one epitope of an AIDS virus envelope protein, thereby being antigenic.