Patent ID: 6395553
Filing Date: 2002-05-28
Classification: B01L,G01N,Y10T

Abstract:
A method for performing an assay on a sample using a disposable analytical device having a reusable centrifugal valve and an active surface, the method comprising:introducing a sample into a reaction chamber of the analytical device; spinning the analytical device at a first rotational speed to generate a radially outward flow of the sample, the first rotational speed being below a threshold rotational speed necessary to actuate the centrifugal valve from a stationary closed position to an open actuated position, whereby the sample in the reaction chamber is prevented from exiting the reaction chamber through the valve, thereby allowing a reaction to occur between the sample and the active surface; and spinning the analytical device at a second rotational speed greater than the first rotational speed to actuate the centrifugal valve from the stationary closed position to the open actuated position, the open actuated position opening the valve, thereby allowing the sample to exit from the reaction chamber through the valve.