Patent ID: 6261816
Filing Date: 2001-07-17
Classification: C07H,C12N,C12P

Abstract:
A method to produce a modified modular polyketide synthase (PKS) which modified PKS is incapable of incorporating a native starter unit substrate for said modular PKS but is able to incorporate a diketide substrate into at least a triketidesaid method comprising culturing recombinant host cells under conditions wherein an encoding nucleotide sequence is expressed to produce said modified modular PKS; andoptionally recovering said modified modular PKS;wherein said recombinant host cells contain a nucleic acid molecule, said nucleic acid molecule comprising a nucleotide sequence encoding at least a first and second module of said PKS, wherein said first module is immediately N-terminal to said second module and wherein said nucleotide sequence compnses a mutation such that the catalytic domain of the functional ketosynthase region of the first module of said encoded PKS is inactivated, said encoding nucleotide sequence operably linked to control sequences for its expression.