Patent ID: 8048654
Filing Date: 2011-11-01
Classification: C10L,C12N,C12P,C12Y,Y02E

Abstract:
1. A method for producing fatty acid esters, comprising: (i) culturing an engineered cyanobacterium in a culture medium, wherein said engineered cyanobacterium comprises a recombinant acyl-CoA synthetase (EC 6.2.1.3) and a recombinant wax synthase (EC 2.3.1.75); and (ii) exposing said engineered cyanobacterium to light and carbon dioxide, wherein said exposure results in the incorporation of an alcohol into fatty acid esters produced by said engineered cyanobacterium, wherein at least one of said fatty acid esters is selected from the group consisting of a tetradecanoic acid ester, a Δ9-hexadecenoic acid ester, a hexadecanoic acid ester, a heptadecanoic acid ester, a Δ9-octadecenoic acid ester, and an octadecanoic acid ester, wherein the amount of said fatty acid esters produced by said engineered cyanobacterium is increased relative to the amount of fatty acid produced by an otherwise identical cell lacking said recombinant acyl-CoA synthetase or recombinant wax synthase.