Patent ID: 9017979
Filing Date: 2015-04-28
Classification: C12N,C12Y

Abstract:
1. A recombinant DNA polymerase having increased reverse transcriptase efficiency, mismatch tolerance, extension rate and/or tolerance of reverse transcriptase (RT) and polymerase inhibitors compared with a control DNA polymerase, wherein the DNA polymerase has at least 98% amino acid sequence identity to SEQ ID NO: 1 and comprises a motif in the polymerase domain consisting of: wherein: X X X X X wherein the amino acid of the DNA polymerase corresponding to position 709 of SEQ ID NO: 1 is selected from the group consisting of K, R, S, G, and A, and the amino acid corresponding to position 580 of SEQ ID NO:1 is selected from group consisting of L, G, T, Q, A, S, N, R, and K, and wherein the control DNA polymerase is the amino acid sequence of SEQ ID NO:1.