Patent ID: 6709812
Filing Date: 2004-03-23
Classification: C12Q

Abstract:
A method for identifying HBV drug resistant strains in a sample, comprising:(i) hybridizing polynucleic acids present in said sample, with a combination of at least a first probe and a second probe, wherein said first probe hybridizes specifically to a mutant HBV RT pol gene target sequence present in said sample, wherein said mutant target sequence comprises at least one codon selected from the group consisting of codons 514, 519-528 and 551-555; and wherein said second probe hybridizes specifically to HBV genotype-specific target nucleic acid sequences in said sample; (ii) detecting the hybrids formed in step (i); (iii) inferring the identity of HBV drug resistant strains in said sample from the differential hybridization signal(s) obtained in step (ii), through discrimination between wild-type and drug resistant HBV strains and through determination of the genotype.