Patent ID: 9040677
Filing Date: 2015-05-26
Classification: C07H,C12N,C12P,C12Q

Abstract:
1. A method for assembling a polynucleotide encoding a transcription activator-like effector (TALE) polypeptide, the method comprising: generating multiple Deoxyribonucleic acid (DNA) segments, an individual DNA segment of the multiple DNA segments corresponding to a repeat sequence of the TALE polypeptide, the repeat sequence identifying a single nucleotide of a particular target polynucleotide or two contiguous nucleotides of the particular target polynucleotide; mixing the multiple DNA segments with restriction enzymes, DNA ligases, and a TALE backbone vector to generate the polynucleotide encoding the TALE polypeptide, a number of multiple DNA segments being greater than 14; and purifying the polynucleotide using a plasmid-safe Deoxyribonuclease (DNase).