Patent ID: 8163559
Filing Date: 2012-04-24
Classification: G01N,Y10T

Abstract:
1. A method for differentiation of white blood cells, wherein the method comprises: obtaining a reagent for differentiation of white blood cells, the reagent comprising a composition configured for four-part differentiation of white blood cells, wherein the composition is free from a surfactant and has an osmolality below about 50 mOsm/kg H mixing the reagent with a whole blood sample in a ratio of 50:1 to 200:1 of reagent:whole blood sample under a temperature between ambient temperature and 40° C. for between 10-20 seconds; detecting information regarding the size and morphology of the white blood cells from the whole blood sample after mixing with the reagent through low-angle scattered light and high-angle scattered light respectively, wherein a detection angle for the low-angle scattered light is between 2-5 degrees and a detection angle for the high-angle scattered light is between 8 -20 degrees; and differentiating the white blood cells into four groups, including three groups corresponding to lymphocytes, monocytes, and eosinophil granulocytes and one group corresponding to neutrophil and basophil granulocytes.