Patent ID: 7932069
Filing Date: 2011-04-26
Classification: A61P,B01D,B01J,C12N

Abstract:
1. A process for producing lactoperoxidase comprising: a step (1) for bringing one or more milk materials, which include lactoperoxidase, into contact with a cation exchanger having weakly acidic groups as ion exchange groups to thereby effect adsorption of the lactoperoxidase; a step (2) for washing the cation exchanger after said adsorption of the lactoperoxidase; a step (3) for bringing said washed cation exchanger into contact with a leaching solvent which elutes the lactoperoxidase, wherein an ionic strength of the leaching solvent is 0.07 to 0.3, to thereby obtain a leaching solution, which includes the lactoperoxidase; a step (4) for concentrating said leaching solution by passing a portion of said leaching solution through an ultrafiltration membrane so that the protein content in said concentrated leaching solution, which is retentate, becomes 0.9 to 15%, and wherein proteins other than the lactoperoxidase precipitate out in the retentate; and a step (5) for obtaining a lactoperoxidase solution by removing the precipitate from the retentate.