Patent ID: 6093542
Filing Date: 2000-07-25
Classification: C07K,C12Q

Abstract:
A method for the detection of quantitation of MDC RNA in a sample, comprising:a) obtaining a sample which may contain MDC RNA;b) performing an isothermal transcription based amplification on the sample with two primers, a first primer being about 15-26 nucleotides in length and comprising at least 10 consecutive nucleotides of a sequence selected from the group consisting of SEQ ID NO:2 and SEQ ID NO:3, and a second primer being about 15-26 nucleotides in length and comprising at least 10 consecutive nucleotides of a sequence selected from the group consisting of SEQ ID NO:6 and SEQ ID NO:7; andc) detecting the amplification product using at least one probe, whereby detection of the amplification product indicates the presence and/or quantity of MDC RNA in the sample.