Patent ID: 9057737
Filing Date: 2015-06-16
Classification: C07K,G01N

Abstract:
1. A kit for quantifying thrombin in a sample comprising anti-thrombin III (AT-III), the kit comprises a thrombin specific antibody or a fragment thereof and a small molecule that recognizes the substrate binding site of thrombin and is selected from the group consisting of naturally occurring hirudin, synthetic hirudin variants, hirudin derivatives, recombinant hirudin variants, mimics, and a combination thereof, wherein the thrombin specific antibody consists of a polyclonal antibody and is raised against a thrombin having a blocked active site with a molecule selected from the group consisting of Phe-Pro-Arg-chloromethylketone (PPACK), 4-aminophenylpyruvic acid (APPA), 4-(2-aminoethyl) benzenesulfonyl fluoride (AEBSF), and combinations thereof.