Patent ID: 8889361
Filing Date: 2014-11-18
Classification: C12Q

Abstract:
1. A method of detecting a circulating tumor cell in a subject comprising: a) providing a sample of blood from said subject; b) enriching cellular adhesion matrix (CAM)-avid cells from said sample by selecting cells from said sample that adhere to a collagen-based cellular adhesion matrix, to provide CAM-enriched circulating cells; and c) detecting increased expression, in said CAM-enriched circulating cells, of each of the genes DPP4, CD44, FAP, KRT8, KRT16, and KRT19, relative to control levels; wherein increased expression of DPP4, CD44, FAP, KRT8, KRT16, and KRT19 in said CAM-enriched circulating cells, relative to control levels, indicates the presence of a circulating tumor cell in said subject.