Patent ID: 8173427
Filing Date: 2012-05-08
Classification: A61K,A61P,C12N

Abstract:
1. An in vitro or ex vivo method of producing a population of post-mitotic cells of the neutrophil lineage, which method comprises the steps of: (a) providing a population of cells comprising neutrophil progenitor cells in an animal cell culture medium and at an initial cell density; (b) culturing the population of cells under conditions of low oxidative stress, wherein the animal cell culture medium comprises (i) one or more early acting cytokines and (ii) one or more cytokines that differentiate said progenitor cells into a neutrophil specific lineage; (c) determining a cell density at which the culture is thereafter to be agitated; and (d) agitating the culture medium during continued culture of the cells once the cells reach the cell density determined in (c) to produce at least a 1000-fold expansion of the population of progenitor cells to post-mitotic cells of the neutrophil lineage.