Patent ID: 8268302
Filing Date: 2012-09-18
Classification: A61K,A61P,C12N

Abstract:
1. A method of reducing motor dysfunction associated with Parkinson's disease in a patient with Parkinson's disease comprising: a) enzymatically dispersing umbilical cord matrix to provide enzymatically dispersed umbilical cord matrix cells; b) culturing the enzymatically dispersed umbilical cord matrix cells in the presence of epidermal growth factor (EGF) and platelet derived growth factor (PDGF) to proliferate the umbilical cord matrix cells; c) culturing the enzymatically dispersed umbilical cord matrix cells on a substrate surface and removing non-adherent cells; d) culturing adherent cells from (c) to select for a population of umbilical cord matrix cells that comprise cells that are negative for CD34 and CD45, positive for telomerase activity, can be expanded in vitro, and maintained in culture through repeated passages; e) isolating the adherent cells from d); f) introducing the isolated adherent cells from e) into the substantia nigra region of the midbrain striatum in a patient with Parkinson's disease, and g) allowing the cells to integrate into the substantia nigra region of the midbrain striatum of the patient whereby motor dysfunction associated with Parkinson's disease in the patient is reduced.