Patent ID: 6716971
Filing Date: 2004-04-06
Classification: C07H,C12Q

Abstract:
An oligonucleotide comprising one or more nucleotide monomers, said monomers having the formula wherein:R1 is a member selected from the group consisting of hydrogen and optionally substituted C1-C6-alkyl; R2 is a member selected from the group consisting of amino and mono- or di-substituted amino wherein the substituent is a protecting group; R3 is optionally substituted C1-C6 alkyl; R4 is L; L is of the formula â€ƒwherein: R5 is a member selected from the group consisting of hydrogen and hydroxyl; R6 is a member selected from the group consisting of hydrogen, a phosphate, a phosphate covalently attached to a nucleotide, a phosphate covalently attached to a nucleoside; a hemisuccinate covalently bound to a solid support, a dicyclohexylcarbodiimide covalently bound to a solid support, and a hydroxyalkyl covalently bound to a solid support; and R7 is a member selected from the group consisting of hydrogen, a phosphate, a phosphate covalently attached to a nucleotide and a phosphate covalently attached to a nucleside; wherein at least one of R6 and R7 is a phosphate covalently attached to adenosine.