Patent ID: 6559284
Filing Date: 2003-05-06
Classification: C07K

Abstract:
A process for purifying biologically active apolipoprotein A (ApoA) or apolipoprotein E (ApoE), or variants or mixtures thereof the improvement comprising mixing to produce a primary aqueous solution a source of ApoA or ApoE, a compound containing two or three nitrogen atoms bonded to a carbon atom, and a composition comprising a first polymeric material and a second polymeric material, and water, wherein die first and second polymeric materials are immiscible in the prima aqueous solution, and the second polymeric material is amphiphilic and water soluble, maintaining the resulting primary aqueous solution for a period of time sufficient for essentially separating the phases formed, removing a phase containing the second polymeric material and a main portion of ApoA or ApoE, and thereafter separating the second polymeric material from the biologically active ApoA or ApoE.