Patent ID: 8603484
Filing Date: 2013-12-10
Classification: A61K,A61P,C07K

Abstract:
1. A method for making a multivalent immunogenic composition comprising polysaccharide-protein conjugates, wherein each of the conjugates comprises a capsular polysaccharide from a different serotype of Streptococcus pneumoniae conjugated to a carrier protein, and the capsular polysaccharides are prepared from serotype 3 and at least one additional serotype, the method comprising: (a) reacting purified serotype 3 polysaccharide with a mild acid resulting in a hydrolyzed serotype 3 polysaccharide; (b) reacting the hydrolyzed serotype 3 polysaccharide with an oxidizing agent in the presence of bivalent cations resulting in an activated serotype 3 polysaccharide; (c) compounding the activated serotype 3 polysaccharide with a carrier protein; (d) reacting the compounded, activated serotype 3 polysaccharide and carrier protein with a reducing agent resulting in a serotype 3 polysaccharide:carrier protein conjugate; and (e) capping unreacted aldehydes in the serotype 3 polysaccharide:carrier protein conjugate resulting in an immunogenic conjugate comprising (f) compounding the immunogenic conjugate comprising