Patent ID: 6479292
Filing Date: 2002-11-12
Classification: A61P,C12N

Abstract:
A method of obtaining a plant cell that contains a predetermined genetic change in a target gene which comprises:(a) introducing into a population of plant cells a compound comprising a single-stranded oligonucleotide having a 3â€² end nucleotide, a 5â€² end nucleotide, and having at least 21 deoxynucleotides and not more then 55 deoxynuclotides and having a sequence comprising at least two regions of at least 8 nucleotides that are each, respectively, identical to two regions of the targeted gene, which regions are separated by at least one nucleotide in the sequence of the targeted gene or in the sequence of the single-stranded oligonucleotide or both, and which regions together are at least 20 nucleotides in length; and (b) identifying a cell of the population having the predetermined genetic change.