Patent ID: 6635456
Filing Date: 2003-10-21
Classification: A61K,C12P

Abstract:
A process for the preparation of phosphatides with the formula (I):Râ€”Oâ€”PO(OH)â€”Oâ€”R1â€ƒâ€ƒ(I) wherein R is diacylglycerol and R1 is an hydroxl group which process comprises:reacting a phosphatide of the formula (II): Râ€”Oâ€”PO(OH)â€”Oâ€”R2â€ƒâ€ƒ(II) wherein R means as above and R2 is CH2â€”CH2â€”NH2 or CH2â€”CH2â€”N(CH3)3, with a primary or secondary alcohol with a chain length of between C2 to C4, optionally substituted with one or more polar groups selected from the group consisting of amino, hydroxy and carboxy, in a single aqueous phase in the presence of an effective amount of phospholipase D with transphosphatidylation activity produced from a Streptomyces hachijoense strain to catalyze the reaction to obtain a phosphatide according to formula (I).