Patent ID: 6025131
Filing Date: 2000-02-15
Classification: C12Q

Abstract:
A method for identifying regulatory regions modulated by a cellular insult comprising:(i) creating a library of gene fusions of genomic DNA fused to a promoterless, luminescent reporter gene complex selected from the group consisting of a gene complex encoding luciferase from Renella species, a thermostable lux gene complex, and a luxCDABE gene complex in Enteric bacteria to create fusion-containing strains;(ii) culturing individual gene fusion-containing strains in liquid media;(iii) contacting the fusion-containing strains at a particular growth phase with a cellular insult; and(iv) analyzing the fusion-containing strain for a change in luminescence relative to a baseline luminescence, said change in luminescence indicating that the fusion-containing strain includes a regulatory region modulated by the cellular insult.