Patent ID: 6245523
Filing Date: 2001-06-12
Classification: A61K,A61P,C07K,G01N

Abstract:
An isolated nucleic acid molecule which encodes a mammalian Survivin protein which inhibits cellular apoptosis operably linked to at least one expression control sequence which effects survivin expression when introduced into a host cell, wherein the nucleic acid molecule hybridizes to the complement of a nucleic acid molecule consisting of nucleotides 2811-2921, 3174-3283, 5158-5275 and 11955-12041 of SEQ ID NO: 35 which encodes SEQ ID # 34, under conditions selected from the group consisting of: (1) washing with 0.015 M NaCl, 0.0015 M sodium titrate, 0.1% NaDodSO.sub.4 at 50.degree. C.; (2) hybridization in 50% (vol/vol) formamide with 0.1% bovine serum albumin, 0.1% Ficoll, 0.1% polyvinylpyrrolidone, 50 mM sodium phosphate buffer at pH 6.5 with 750 mM NaCl, 75 mM sodium citrate at 42.degree. C.; and (3) hybridization in 50% formamide, 5X SSC (0.75 M NaC1, 0.075 M sodium citrate), 50 mM sodium phosphate (pH 6.8), 0.1% sodium pyrophosphate, 5X Denhardt's solution, sonicated salmon sperm DNA (50 .mu.g/ml), 0.1% SDS, and 10% dextran sulfate at 42.degree. C., with washes at 42.degree. C. in 0.2X SSC and 0.1% SDS.