Patent ID: 8790890
Filing Date: 2014-07-29
Classification: A61B,A61K,C12M,G01N,G16B

Abstract:
1. A method of determining tissue regenerative ability of the skin of a subject, comprising: (i) cultivating skin fibroblast colonies from the subject at conditions which provide formation of discrete colonies applicable for visualization, (ii) determining efficacy of colony formation as a parameter that defines regenerative potential of a fibroblast population, wherein the efficacy of colony formation is calculated as a ratio of the formed cells with the number of cells >20 to the total number of explanted cells, and if efficacy of colony formation is less than 45% for men and 36% for women, low regenerative potential is determined; if efficacy of colony formation is in the range 45-49% for men and 36-45% for women, normal regenerative potential is determined, and if efficacy of colony formation is more than 49% for men and 45% for women, high regenerative potential is determined; and (iii) determining percentages of dense and diffuse colonies in cell culture, as a parameter that defines proliferative cell potential, wherein the dense colonies are characterized by mean of optical density >46 relative units (rel.un.), and the diffuse colonies are ≦25 rel.un. and if percentage of the dense colonies is less than 44%, and percentage of the diffuse colonies is more than 25% for men, and percentage of the dense colonies is less than 40%, and percentage of the diffuse colonies is more than 40% for women, low proliferative potential is determined, if percentage of the dense colonies is in the range 44-54%, and percentage of the diffuse colonies is in the range 20-25% for men, and percentage of the dense colonies is in the range of 40-50%, percentage of the diffuse colonies is in the range 30-40% for women, normal proliferative potential is determined; if percentage of the dense colonies is more than 54%, and percentage of the diffuse colonies is less than 20% for men, and percentage of the dense colonies is more than 50%, and percentage of the diffuse colonies is less than 30% for women, high proliferative potential is determined; and wherein a low regenerative potential and/or a low proliferative cell potential of the fibroblast population is indicative of abnormal tissue regenerative ability and a high regenerative potential and a high proliferative cell potential of the fibroblast population is indicative of quick tissue regenerative ability.