Patent ID: 6361978
Filing Date: 2002-03-26
Classification: C12N,C12P

Abstract:
A process for making biotin comprising the steps of:(a) contacting desthiobiotin with an enzyme reaction mixture comprising: (i) a bioB gene product, (ii) an additional gene product selected from the group consisting of a nifU gene product, a nifS gene product, and a combination thereof, and (iii) an effective amount to form biotin of: (1) S-adenosylmethionine, (2) L-cysteine, and (3) a first electron donor system comprising ferredoxin-NADP reductase, flavodoxin, and NADPH or a second electron donor system selected from the group consisting of deazariboflavin, deazaflavin, and 8-hydroxy-5-deazaflavin, wherein the second electron donor system is reduced; and (b) isolating biotin from the reaction mixture.