Patent ID: 8486622
Filing Date: 2013-07-16
Classification: B82Y,C12Q,Y10T

Abstract:
1. A method of detecting a nucleic acid sequence in a genomic sample, said method comprising: providing the genomic sample comprising a quantity of duplex nucleic acids containing target nucleic acid sequences; providing a quantity of probes comprising probe nucleic acid sequences; providing a hybridization mixture comprising the genomic sample, the quantity of probes, a quantity of hybridization promoting agents and labels; incubating the hybridization mixture to provide an incubated mixture comprising complexes of the duplex nucleic acids, the probes and the labels; irradiating the incubated mixture with radiation effective to stimulate at least some of the labels to emit energy; and detecting from a fluorescent signal whether the probe nucleic acid sequences perfectly match the target nucleic acid sequences, to thereby detect whether the nucleic acid sequence is present in the genomic sample, wherein a ratio of the quantity of probes to the quantity of duplex nucleic acids is at least 10