Patent ID: 6342365
Filing Date: 2002-01-29
Classification: C12N,C12Y,Y10S

Abstract:
A method of screening for linkage-specific glycosidase activity, said method comprising the steps of:(a) reacting at least two labeled oligosaccharide substrates with a preparation from an organism, wherein said oligosaccharide substrates comprise both anomeric forms of saccharide linkage or more than one form of adjacent saccharide linkage; (b) detecting the products of the reaction in step (a); and (c) screening for the presence of a glycosidase selected from the group consisting of a glycosidase which specifically cleaves one anomeric form of at least one of said oligosaccharide substrates but not the other anomeric form and a glycosidase which specifically cleaves one form of adjacent saccharide linkage of at least one of said oligosaccharide substrates but not the other form of adjacent saccharide linkage.