Patent ID: 6214539
Filing Date: 2001-04-10
Classification: A61K,C07K,C12N,Y10S

Abstract:
A method for determining the presence of antibodies to LAV/HTLV-III in a physiological fluid, said method comprising:introducing a human serum, plasma or blood sample into a sample container coated at least in part with a first or a subsequent peptide having from eight to about thirty-six amino acids which come within the sequence of at least one of the following peptide sequences:(I) (15) Y-Asp-Cys-Lys-Thr-Ile-Leu-Lys-Ala-Leu- Gly-Pro-Ala-Ala-Thr-Leu-Glu-Glu-Met-Met- Thr-Ala-Cys-X (II) (17) Y-Leu-Lys-Glu-Thr-Ile-Asn-Glu-Glu-Ala- Ala-Glu-Trp-Asp-Arg-Val-His-Pro-Val-His- Ala-X (III) (92) Y-Asp-Arg-Val-His-Pro-Val-His-Ala-Gly-Pro- Ile-Ala-Pro-Gly-Gln-X (IV) (90) Y-Tyr-Ser-Pro-Thr-Ser-Ile-Leu-Asp-Ile-Arg- Gln-Gly-Pro-Lys-Glu-Pro-Phe-Arg-Asp-Tyr-Val- Asp-Arg-Phe-Tyr-Lys-Thr-Leu-Arg-Z-X (V) (88) Y-Asn-Trp-Nor-Thr-Glu-Thr-Leu-Leu-Val-Gln- Asn-Ala-Asn-Pro-Asp-Cys-Lys-Thr-Ile-Leu-Lys- Ala-Leu-Gly-Pro-Ala-Ala-Thr-Leu-Glu-Glu-Nor- Nor-Thr-Ala-Cys-X (VI) (97) Y-Arg-Glu-Leu-Glu-Arg-Phe-Ala-Val-Asn-Pro-Gly- Leu-Leu-Glu-Thr-Ser-Glu-Gly-Cys-Arg-Gln-Ile- Leu-Gly-Gln-Leu-Gln-Pro-Ser-Leu-Gln-Thr-X (VII) (71) Y-Asp-Thr-Gly-His-Ser-Ser-Gln-Val-Ser-Gln- Asn-Tyr (XI) (40) Y-Lys-Ser-Leu-Glu-Gln-Ile-Trp-Asn-Asn- Met-Thr-Trp-Met-Glu-Trp-Asp-Arg-Glu- Ile-Asn-Z-X (XII) (23) Y-His-Ser-Leu-Ile-Glu-Glu-Ser-Gln-Asn- Gln-Gln-Glu-Lys-Asn-Glu-Gln-Glu-Leu-Leu- Glu-Leu-Asp-Lys-Trp-Z-X (XIII) (79) Y-Lys-Asp-Gln-Gln-Leu-Leu-Gly-Ile-Trp-Gly- Cys-Ser-Gly-Lys-Leu-Ile-Cys-X,where X is OH or NH.sub.2, and Y and Z, if present, are amino acids added to facilitate coupling, wherein said first or subsequent peptides are free of other peptides or conjugated to a macromolecule for which antibodies in human sera are substantially absent;incubating for a sufficient time for complex formation to occur; anddetermining the formation of complex by employing a labeled specific binding protein which binds to said complex and provides a detectable signal.