Patent ID: 7416866
Filing Date: 2008-08-26
Classification: C12N,C12P

Abstract:
1. A process for selective introduction of a double bond into ring A of a steroid skeleton by overexpression of 3-keto steroid-Δ 1 -dehydrogenases , wherein a) a Δ b) promoter and terminator elements of the 3-keto steroid-Δ c) expression plasmids are constructed in which the 3-keto steroid-Δ d) e) the thus produced bacteria are cultivated, and the selective dehydrogenation at 1-position in the steroid skeleton of hydrocortisone (F), hydrocortisone-17-acetate (MAF), hydrocortisone-21-acetate (EAF), 4-androstene-3-17-dione(AD), fluocortolone A acetate (FCAA) or 11β,17α-Dihydroxy-6α,9α-difluoro-16α-methylprogesterone (DDFMP) is performed with these cultures, whereby