Patent ID: 9028813
Filing Date: 2015-05-12
Classification: A61K,C12N

Abstract:
1. A method of degrading or inhibiting one or more botulinum neurotoxin (BoNT) serotypes that have intoxicated one or more cells, wherein the method comprises contacting an amount of an isolated recombinant ubiquitin ligase molecule with the intoxicated cells, wherein the isolated recombinant ubiquitin ligase molecule comprises: a. a non-cleavable SNAP-25 domain that binds to an enzymatically active fragment of a BoNT, wherein the non-cleavable SNAP-25 domain consists essentially of an amino acid sequence selected from the group consisting of: b. an E3-ligase domain that comprises an E3-ligase or polypeptide that facilitates E2-mediated ubiquitination of the enzymatically active fragment of a BoNT, wherein the isolated recombinant ubiquitin ligase molecule degrades or inhibits at least one BoNT serotype.