Patent ID: 6207436
Filing Date: 2001-03-27
Classification: C11D,C12N,C12Y,D06M,D06P

Abstract:
An endo-.beta.-1,4-glucanase derived from a strain belonging to the genus Saccharothrix, comprising one of:(a) a polypeptide sequence comprising (i) a cellulose binding domain (CBD), (ii) a catalytically active domain (CAD), and (iii) a linking region encoded by a polynucleotide sequence comprising nucleotides 436-675 of SEQ ID NO:1, wherein the linking region operably linking the CBD and the CAD of the endo-.beta.-1,4-glucanase;(b) a polypeptide exhibiting endo-.beta.-1,4-glucanase activity encoded by a polynucleotide sequence which hybridizes with the polynucleotide sequence encoding the polypeptide of (a) under the conditions of hybridization at 45.degree. C. for 12 hrs, followed by repeated washing in 2.times.SSC, 0.5% SDS at 55.degree. C.-75.degree. C., and(c) a polypeptide comprising the amino acid sequence of positions 20-490 of SEQ ID NO:2.