Patent ID: 8642836
Filing Date: 2014-02-04
Classification: C12N,C12P

Abstract:
1. A method of using D-phosphinothricin as a hybridization agent to produce dicotyledon hybrid seed, comprising the steps of: a. providing conditionally female sterile transgenic plants comprising a transgene, expressed by a tissue specific female floral promoter, said transgene encoding a mutant D-amino acid oxidase derived from b. applying exogenously to the conditionally female sterile transgenic plants an amount of D-phosphinothricin at an appropriate time; c. selecting for the conditionally female sterile transgenic plants which produce little or no seed after application of D-phosphinothricin and which produce at least near normal levels of viable pollen; d. pollinating a female fertile plant with the pollen of step (c); and e. harvesting dicotyledon hybrid seed from the plant of step (d).