Patent ID: 9169464
Filing Date: 2015-10-27
Classification: A61K,C12M,C12N

Abstract:
1. A stem cell isolation kit comprising: a) a first bag containing a plasma blood expander; b) a second bag containing an anticoagulant and a growth medium, said second bag comprising an injection port for introduction of a biological sample comprising red blood cells and stem cells; c) a third bag for collection of sedimented red blood cells from the second bag; d) a fourth bag comprising an interior surface coated with a cell-adherent surface layer for collection and isolation of adherent stem cells; e) a fifth bag containing a preservative for storage of adherent stem cells; wherein said first and second bags are interconnected with each other through sterile tubing for transferring the contents of the first bag to the second bag; the second and third bags are interconnected with each other through sterile tubing for sedimenting red blood cells from the second bag to the third bag; the second and fourth bags are interconnected with each other through sterile tubing for transferring of stem cells from the second bag to the fourth bag; and wherein the fourth and fifth bags are interconnected with each other through sterile tubing for the transfer of adherent stem cells from the fourth to the fifth bag.