Patent ID: 6828297
Filing Date: 2004-12-07
Classification: A61K,A61P

Abstract:
A mixture of conjugates, each comprising human insulin coupled to an oligomer that comprises a polyethylene glycol moiety, wherein the mixture has a dispersity coefficient (DC) greater than 10,000 where DC=(&Sum;i=1n&it;Ni&it;Mi)2&Sum;i=1n&it;Ni&it;Mi2&it;&Sum;i=1n&it;Ni-(&Sum;i=1n&it;Ni&it;Mi)2wherein: n is the number of different molecules in the sample; Ni is the number of ith molecules in the sample; and Mi is the mass of the ith molecule; and wherein the conjugate comprises a first oligomer and a second oligomer; and wherein the first oligomer is covalently coupled at LysB29 of the insulin and the second oligomer is covalently coupled at N-terminal A1 or N-terminal B1 of the insulin.