Patent ID: 7741043
Filing Date: 2010-06-22
Classification: C07K,C12Q,G01N

Abstract:
1. A method for identifying therapeutic agents that inhibit, potentiate or mimic the ability of a km23 polypeptide mutant form, or a nucleic acid encoding the polypeptide mutant form to modulate a signal transduction activity of a growth factor or cytokine, the method comprising: (i) treating a cell with an effective amount of at least one candidate so as to alter at least one of the group consisting of regulation of gene expression, cell growth, cell differentiation, cell survival, apoptosis, senescence, cell migration, angiogenesis, fibrosis, extracellular matrix induction, adhesion, autoproduction and combinations thereof; and (ii) measuring the effect of the candidate on the cell; wherein said mutant form modulates TGFβ signaling events or dynein intermediate chain (DIC) binding activity and is a km23 polypeptide comprising SEQ ID NO: 1, except that the polypeptide lacks amino acids corresponding to amino acids 27-82 in SEQ ID NO: 1.