Patent ID: 7842856
Filing Date: 2010-11-30
Classification: C12N

Abstract:
1. A method for producing a genetically engineered, non-transgenic, herbicide resistant or tolerant plant cell, said method comprising the steps of: (a) introducing into a plant cell a recombinagenic oligonucleotide with a targeted mutation in the protoporphyrin oxidase (PPO) gene to produce plant cells with a mutant PPO gene that expresses a mutant PPO protein that is deleted at an amino acid position corresponding to Gly210 or Gly 211 in the (b) identifying a plant cell having a mutant PPO protein that has substantially the same catalytic activity as compared to a corresponding wild type PPO protein in the presence of herbicide except that said mutant PPO protein is not inhibited by the herbicide; and whereby a non-transgenic herbicide resistant plant cell is produced.