Patent ID: 9181524
Filing Date: 2015-11-10
Classification: A61K,A61P,C12N

Abstract:
1. A method for the differentiation of human embryonic stem (hES) cells, said method comprising: a) culturing an embryoid body (EB), produced from hES cells, for a sufficient time for the appearance of pigmented cells comprising brown pigment dispersed in their cytoplasm, under conditions that do not maintain stem cells in an undifferentiated state, wherein the hES cells are stem cells that express Oct-4, alkaline phosphatase, SSEA-3, SSEA-4, TRA-I-60 and TRA-I-81; and b) isolating and culturing the pigmented cells under adherent conditions to form a population of cells that expresses bestrophin, CRALBP, PEDF1 and RPE65.