Patent ID: 6214588
Filing Date: 2001-04-10
Classification: C07K,C12N,G01N

Abstract:
A purified multisubunit RNA polymerase II holoenzyme wherein the holoenzyme comprises at least eight SRB polypeptides, wherein the polypeptides are selected from the group consisting of SRB2, SRB4, SRB5, SRB6, SRB7, SRB8, SRB9, SRB10, and SRB11, wherein at least one of the SRB polypeptides binds specifically to a polyclonal antibody raised against S. cerevisiae SRB2, SRB4, SRB5, SRB6, SRB7, SRB8, SRB9, or human SRB7, general transcription factors b, e and g, RNA polymerase II, and at least one SWI/SNF protein, the holoenzyme having a molecular weight of approximately 1-4 Md.