Patent ID: 6150415
Filing Date: 2000-11-21
Classification: A61K,A61P,G01N

Abstract:
A method of treating an epoxide hydrolase, useful to purify, isolate, or inhibit the epoxide hydrolase, comprising:providing a compound having the structure ##STR38## wherein X and Y is each independently nitrogen, oxygen, or sulfur, and X can further be carbon, at least one of R.sub.1 -R.sub.4 is hydrogen, R.sub.2 is hydrogen when X is nitrogen but is not present when X is sulfur or oxygen, R.sub.4 is hydrogen when Y is nitrogen but is not present when Y is sulfur or oxygen, R.sub.1 and R.sub.3 is each independently H, C.sub.1-20 substituted or unsubstituted alkyl, cycloalkyl, aryl, acyl, or heterocyclic, the compound provided being in free form or being derivatized so as to be immobilized to a water insoluble support; and,contacting the free form or immobilized compound with an epoxide hydrolase under conditions in which the epoxide hydrolase is enzymatically active, the contacting effective to form a complex between the compound and the epoxide hydrolase, wherein the activity of the epoxide hydrolase so complexed is modified with respect to enzymatically active, uncomplexed epoxide hydrolase.