Patent ID: 6526298
Filing Date: 2003-02-25
Classification: A61B,G01N

Abstract:
A method for establishing a calibration relationship to determine the concentration of an analyte or a disease state in a biological tissue, said method comprising the steps of:(a) selecting a sampling area on the surface of said biological tissue; (b) setting the temperature of said sampling area of said biological tissue to a first temperature; (c) introducing light at a light introduction site, said light introduction site being within said sampling area and collecting light re-emitted at a light collection site, said light collection site being within said sampling area, said light introduction site and said light collection site being separated by a sampling distance, said introduced light being within a first wavelength range; (d) performing at least one optical measurement at said sampling distance; (e) setting the temperature of said sampling area of said biological tissue to a second temperature, said second temperature being different from said first temperature; (f) repeating steps (c) and (d) at said second temperature, said introduced light at said second temperature being within a second wavelength range; (g) determining the value of at least one optical parameter at said first temperature and at at least one wavelength within said first wavelength range and the value of said at least one parameter at said second temperature and at at least one wavelength within said second wavelength range; and (h) establishing a mathematical relationship that relates the value of said at least one optical parameter at said first temperature and at said at least one wavelength within said first wavelength range and the value of said at least one optical parameter at said second temperature and at said at least one wavelength within said second wavelength range with an independently measured concentration of said analyte or an independent measurement of said disease state.