Patent ID: 7897330
Filing Date: 2011-03-01
Classification: B01L,C12Q,G01N

Abstract:
1. A method for determining one of a mechanism of inhibition, potency of an inhibitor, and both a mechanism of inhibition and potency of an inhibitor of a biological molecule, comprising: (a) contacting at least one inhibitor, a biological molecule, wherein the biological molecule comprises an enzyme, and at least one ligand for the biological molecule under conditions where concentrations of at least two of the at least one inhibitor, the biological molecule and the at least one ligand are simultaneously varied over a continuous concentration gradient; (b) measuring the enzymatic reaction over the continuous concentration gradient; and (c) determining an outcome of the contacting of the at least one inhibitor, the at least one ligand, and the biological molecule to determine one of the mechanism of inhibition, potency of the inhibitor, and both the mechanism of inhibition and potency of the inhibitor, wherein determining the outcome comprises determining the inhibition constant of the at least one inhibitor.