Patent ID: 7575881
Filing Date: 2009-08-18
Classification: C12Q,G01N

Abstract:
1. A method of identifying an agent that modulates arginine-tRNA protein transferase mediated N-end rule pathway mediated arginylation of an amino terminal (N-terminal) cysteine (Cys) residue of a peptide, comprising: a) contacting at least one sample comprising a peptide consisting of the amino sequence Cys-His-Ser-Gly-Ala-Trp-Leu (SEQ ID NO:1) or a fusion protein comprising said peptide fused to a reporter polypeptide, with at least one test agent, under conditions suitable for the N-terminal Cys of the peptide to act as a substrate for an N-end rule pathway reaction; and b) detecting a change in the N-end rule pathway substrate activity of the N-terminal Cys of the peptide in the presence of the test agent as compared to the absence of the test agent; and c) correlating the change in the N-end rule pathway substrate activity with a modulation of the N-end rule pathway substrate activity, thereby identifying the test agent as an agent that modulates N-end rule pathway mediated arginylation of an N-terminal Cys residue of said peptide.