Patent ID: 6627408
Filing Date: 2003-09-30
Classification: G01N,Y10S

Abstract:
A method of identifying a candidate agent as a modulator of function of a tar herein said target protein comprises a protein selected from the group consisting of Schizosaccharomyces pombe Cut7; Saccharomyces cerevisiae CIN8; and Saccharomyces cerevisiae KIP1; or a fragment of said target protein and said method comprises:a) adding a candidate agent to a mixture comprising said target protein that directly or indirectly produces ADP or phosphate under conditions which normally allow the production of ADP or phosphate; b) subjecting the mixture to an enzymatic reaction that uses said ADP or phosphate as a substrate under conditions which normally allow the ADP or phosphate to be utilized; and c) determining the level of activity of the enzymatic reaction wherein a change in said level between the presence and absence of said candidate agent indicates a modulator of said target protein function.