Patent ID: 7297961
Filing Date: 2007-11-20
Classification: G02B

Abstract:
1. A fluorescence microscope, comprising: an objective lens; a first medium unit with a refractive index of n a third medium unit with a refractive index of n an image processing unit for providing images of objects obtained due to fluorescence collected by the objective lens as images; a prism including at least an incident surface, a contact surface and an output surface; a light source for providing excitation light to the incident surface of the prism; an optical coupling unit formed on the contact surface of the prism so as to allow a sample including one or more micro-objects having fluorophores to be seated thereon; and an absorber installed on the output surface of the prism to absorb the excitation light that has been totally reflected, wherein the refractive indices of the third and first medium units satisfy a relationship of n wherein an incident angle of the excitation light on the interface is equal to or greater than a critical angle, and wherein fluorescences, emitted when fluorophores present in micro-objects that exist in the path of the excitation light within the first medium unit are excited, are captured by the objective lens, thus observing the micro-objects.