Patent ID: 8822419
Filing Date: 2014-09-02
Classification: A61K,A61P,C07K,G01N

Abstract:
1. A method of selectively necrosing cells, comprising: providing a plurality of cells, including at least one cancer cell and at least one non-cancerous cell, wherein the at least one cancer cell has increased expression of HDM-2; administering to the cells a compound, including a HDM-2 targeting component which is a peptide and a cytotoxic component which is a membrane resident peptide, said cytotoxic component attached to said HDM-2 targeting component at the carboxy terminus; wherein the compound binds to said at least one cancer cell and causes pore formation in a membrane thereof and wherein the compound is selected from the group consisting of PNC-27 and PNC-28; and measuring in a cell medium contacted with the cells a level of LDH, whereby an increase in the level of LDH in the cell medium after administering the compound as compared to the level of LDH in the cell medium prior to administering the compound indicates necrosis of said at least one cancer cell.