Patent ID: 8039213
Filing Date: 2011-10-18
Classification: C12Q

Abstract:
1. A method of predicting responsiveness of a cancer patient to a drug candidate comprising: preparing multicellular tumor tissue explants from patient specimens; allowing the multicellular tumor tissue explants to form cell culture monolayers, and removing the explants from the culture when the monolayer is about 10 to about 50 percent confluency and growing the monolayer, thereby preventing substantial growth of stromal cells; optionally preparing a subculture from the monolayer cells; conducting at least one phenotypic assay on cells from the monolayers or the subcultures, the phenotypic assay being predictive of the drug's efficacy; and optionally conducting at least one genotypic analysis of cells from the patients to determine the presence or absence of a genotype predictive of resistance to said drug; thereby predicting responsiveness to the drug candidate.