Patent ID: 6121246
Filing Date: 2000-09-19
Classification: A61K,A61P

Abstract:
A method for inducing the formation of new blood vessels in an ischemic muscle tissue in a human host, comprising:(a) selecting a human host in need of increased blood flow in an ischemic muscle tissue, and(b) directly injecting into said tissue of said human host an effective amount of a DNA sequence encoding an angiogenic protein or a modified angiogenic protein, wherein said DNA sequence comprises a promoter sequence and either a native secretory signal sequence or an operably linked secretory signal sequence, wherein the angiogenic protein or modified angiogenic protein is selected from the group consisting of acidic and basic fibroblast growth factors, vascular endothelial growth factor, endothelial cell growth factor, epidermal growth factor, transforming growth factor .alpha. and .beta., platelet-derived endothelial growth factor, platelet-derived growth factor, tumor necrosis factor .alpha., hepatocyte growth factor, and insulin-like growth factor, and wherein said effective amount of said DNA sequence expresses sufficient angiogenic protein in said tissue to induce new blood vessel formation in said human host.