Patent ID: 7074595
Filing Date: 2006-07-11
Classification: C12N

Abstract:
1. A process for the production of selenomethionine-labeled protein, comprising the steps of: a) providing a cell-free protein synthesis system comprising a reactor for carrying out protein synthesis by a batch process or a dialysis process, and wherein the cell-free protein synthesis system includes a wheat embryo extract for protein synthesis from which endosperm has been completely removed and in which at least about 80% of the methionine in the wheat embryo extract has been replaced by selenomethionine, and a protein synthesis reaction solution for cell-free protein synthesis in which at least about 80% of the methionine in the protein synthesis reaction solution has been replaced by selenomethionine, and wherein surfaces of the reactor that contact the protein synthesis reaction solution have been subjected to a coating treatment with a coating protein, and b) carrying out cell-free protein synthesis with the cell-free protein synthesis system by a batch process or a dialysis process whereby a template mRNA is translated into a selenomethionine-labeled protein.