Patent ID: 7419786
Filing Date: 2008-09-02
Classification: C12Q

Abstract:
1. A method for monitoring PCR amplification of a target nucleic acid, which comprises: conducting a PCR reaction with a forward primer, a reverse primer, and a nucleic acid probe which is labeled with a fluorescent dye, wherein only said nucleic acid probe is provided for one type of target nucleic acid, wherein said probe is labeled at an end portion thereof with said fluorescent dye, and said probe has a base sequence designed such that, when said probe is hybridized with said target nucleic acid, at least one G (guanine) base exists in a base sequence of said target nucleic acid at a position 1 to 3 bases apart from an end base portion where said probe and said target nucleic acid are hybridized with each other; wherein said fluorescent dye is reduced in fluorescence emission when said probe is hybridized with said target nucleic acid or amplified target nucleic acid; and monitoring a time-course of said amplification of the target nucleic acid by measuring a fluorescence intensity in said PCR reaction.