Patent ID: 8653046
Filing Date: 2014-02-18
Classification: A61K,A61P,C12N

Abstract:
1. An in vitro method for controlling transgene expression, the method comprising: (i) transfecting isolated mammalian cells with a first vector comprising a polynucleotide sequence operably linked to a constitutively active promoter sequence, wherein said polynucleotide sequence encoding a fusion protein consisting of a transfecting said isolated mammalian cells with a second vector comprising a TtgR-specific operator (O or transfecting isolated mammalian cells with a single vector comprising a first polynucleotide sequence operably linked to a constitutively active promoter sequence, wherein said polynucleotide sequence encoding a fusion protein consisting of a TtgR and a transactivation domain, or a fusion protein consisting of a TtgR and a transrepressor domain; and a second polynucleotide sequence comprising an O (ii) contacting said cells with a non-toxic micromolar concentration range of a skin permeating compound selected from phloretin and butylparaben, wherein said skin permeating compound dissociates TtgR-transactivation domain fusion protein from O