Patent ID: 8728761
Filing Date: 2014-05-20
Classification: C07K,C12N,C12P,C12Y

Abstract:
1. A method for producing at least one glycosylated polypeptide, comprising the steps of: (i) culturing a transformed microalgae so as to obtain the expression of said at least one glycosylated polypeptide; (ii) purifying glycosylated polypeptides from the cultured microalgae; (iii) determining the glycosylation pattern of the glycosylated polypeptides; and (iv) selecting said at least one glycosylated polypeptide having at least one Man said transformed microalgae comprises a nucleotide sequence encoding said at least one glycosylated polypeptide operably linked to a promoter that drives expression of said nucleotide sequence in said microalgae, and the transformed microalgae do not have suppressed expression of β(1,2)-xylosyltransferase.