Patent ID: 6599704
Filing Date: 2003-07-29
Classification: C12Q,G01N

Abstract:
A method for detecting mutated alleles in an excess of unmutated wild typed alleles, the method comprising:isolating from an examination sample a DNA sample comprising unmutated wild typed alleles and suspected mutated alleles, wherein the nucleotide sequence is known for the unmutated wild typed alleles; amplifying the DNA sample by PCR; introducing the amplified DNA sample into at least one chromatographic column wherein the at least one chromatographic column comprises at least one carrier-bound oligonucleotide sequence that either alone in a single column or in multiple columns is complementary to the entire region of interest of the unmutated wild type sequence suspected of including at least one point mutation, and wherein the wild type alleles bind to the carrier bound oligonucleotide DNA sequences and the mutated alleles do not bind under hybridizing conditions maintained at the melting temperature of the wild-type specific oligonucleotide and its complementary sequence or if the mutated alleles do bind they are eluted from the column before the wild type alleles when a buffer is added; and detecting the mutation in the mutated alleles eluted from the at least one chromatographic column.