Patent ID: 7723269
Filing Date: 2010-05-25
Classification: C07K

Abstract:
1. A process for selecting anti-angiogenesis antibody fragments comprising: (i) distributing bacterial colonies expressing billions of different binding specificities in soluble form onto a growth filter, wherein all of said bacterial colonies are grown until completely confluent and are present on said growth filter and said binding specificities comprise antibody fragments; (ii) incubating the growth filter until the colonies are confluent; (iii) providing a trap filter comprising a trapped antigen; (iv) placing the trap filter in a plate containing culture medium; (v) covering the trap filter with the growth filter with the bacterial colonies pointing upwards; (vi) incubating the growth filter and the trap filters, such that antibody fragments expressed by the colonies on the growth filter bind to the antigen on the trap filter; (vii) detecting the binding of antibody fragments to the trap filter; (viii) identifying the bacterial colonies on the growth filter which expressed the antibody fragments bound to the trap filter; (viii) identifying the bacterial colonies on the growth filter which expressed the antibody fragments bound to the trap filter, while avoiding the display of antibodies on phage (ix) growing the identified bacterial colonies in liquid medium; (x) distributing said identified bacterial colonies onto a membrane filter; and (xi) repeating steps (iii) to (x) for additional cycles until single colonies expressing antibody fragments which bind to the trap filter are identified in step (viii), said single colonies producing soluble anti-angiogenesis antibody fragments.