Patent ID: 6703489
Filing Date: 2004-03-09
Classification: A61K,C07K

Abstract:
An isolated antibody which binds a first Serrate protein, which first Serrate protein (i) binds a Notch protein, and (ii) is encoded by a first nucleic acid that hybridizes under low stringency conditions to a second nucleic acid, which second nucleic acid consists of a nucleotide sequence selected from the group consisting of the mouse Serrate sequence of SEQ ID NO:15, the antisense strand to the mouse Serrate sequence of SEQ ID NO:15, the mouse Serrate sequence of SEQ ID NO:17, the antisense strand to the mouse Serrate sequence of SEQ ID NO:17, the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the antisense strand to the chick Serrate sequence depicted in FIGS. 11A-11B (SEQ ID NO:9), the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), and the antisense strand to the human Serrate sequence depicted in FIGS. 9A-9G (SEQ ID NO:5), said low stringency conditions comprising hybridization in a buffer comprising 35% formamide, 5Ã—SSC, 50 mM Tris-HCl (pH 7.5), 5 mM EDTA, 0.02% PVP, 0.02% Ficoll, 0.2% BSA, 100 &mgr;g/ml denatured salmon sperm DNA, and 10% (wt/vol) dextran sulfate, for 18-20 hours at 40Â° C., washing in a buffer consisting of 2Ã—SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0. 1% SDS, for 1.5 hours at 55Â° C., and washing in a buffer consisting of 2Ã—SSC, 25 mM Tris-HCl (pH 7.4), 5 mM EDTA, and 0.1% SDS, for 1.5 hours nat 60Â° C., which antibody does not bind a Drosophila Serrate protein.