Patent ID: 7504239
Filing Date: 2009-03-17
Classification: C12Q

Abstract:
1. A method of differentiating bottom-fermenting yeast from wild yeast comprising synthesizing a pair of primers consisting of (i) a primer consisting of the base sequence set forth in SEQ ID NO: 7 and (ii) a primer consisting of 15-30 nucleotides of a sequence that is complementary to a base sequence of chromosome IX of performing PCR (Polymerase Chain Reaction) to produce a PCR amplification product, wherein the primers for said PCR consist of said pair of primers, the template for said PCR is a DNA separated from a yeast specimen, and said PCR is performed by denaturing for 10 seconds to 2 minutes at a temperature of 90-98° C., annealing for 20 seconds to 2 minutes at a temperature of 40-75° C., and extending for 1-20 minutes at a temperature of 65-75° C., with 10-30 cycles; and differentiating whether said yeast is bottom-fermenting yeast or wild yeast, based on the PCR amplification product, wherein said wild yeast is yeast not used for brewing and said wild yeast belongs to a genus selected from the group consisting of the genus wherein said bottom-fermenting yeast is