Patent ID: 7920972
Filing Date: 2011-04-05
Classification: G01N,G01R,G16B

Abstract:
1. A method of conducting a (5,3) dimensional (D) [HN{ N , CO }{ C αβ C α }] G-matrix Fourier transformation (GFT) nuclear magnetic resonance (NMR) experiment, said method comprising: providing a sample, wherein said sample is a protein molecule having two consecutive amino acid residues, i−1 and i, and the chemical shift values for the following nuclei are measured: (1) α- and β-carbons of amino acid residues i and i−1, applying radiofrequency pulses for a first 5D FT NMR experiment to the sample; using a spectrometer; selecting a first set of 2 indirect chemical shift evolution periods of the 5D FT NMR experiment, jointly sampling the first set of 2 indirect chemical shift evolution periods in a first indirect time domain dimension, t selecting a second set of 2 indirect chemical shift evolution periods of the 5D FT NMR experiment, jointly sampling the second set of 2 indirect chemical shift evolution periods in a second indirect time domain dimension, t independently cosine and sine modulating NMR signals detected in a direct dimension to generate 3D basic NMR spectra comprising frequency domain signals having a chemical shift multiplet with 4 components resulting from each of 2 chemical shift doublet components in a first frequency domain dimension, ω transforming the 3D basic NMR spectra into 3D phase-sensitively edited basic NMR spectra, wherein the 4 chemical shift multiplet components of the 3D basic NMR spectra are edited to yield 3D phase-sensitively edited basic NMR spectra having individual chemical shift multiplet components.