Patent ID: 6312905
Filing Date: 2001-11-06
Classification: C12Q,Y10T

Abstract:
A denaturation fingerprinting method for detecting mutations in a nucleic acid segment which comprisessubjecting the nucleic acid segment to bidirectional sequencing using oppositely oriented primers that bound the segment and two different dideoxynucleotide terminators in a single reaction,wherein one of the dideoxynucleotide terminators is either ddATP or ddTTP and the other is either ddGTP or ddCTP, andwherein one of the dideoxynucleotide terminators is modified so as to alter the electrophoretic mobility of fragments containing a residue of the modified dideoxynucleotide terminator relative to fragments containing the corresponding unmodified dideoxynucleotide terminator, andseparating the resulting fragments by denaturing electrophoresis in one lane to produce a fingerprint pattern.