Patent ID: 6335165
Filing Date: 2002-01-01
Classification: C12Q

Abstract:
A method of characterizing a GC rich region of a nucleic acid of interest comprising the steps of:(a) demethylating said GC rich region of the nucleic acid of interest; (b) contacting the nucleic acid of interest with an agent that modifies cytosine or guanine residues into residues complementary to adenine or thymine for obtaining a modified nucleic acid in which said cytosine or guanine residues are replaced by said residues complementary to adenine or thymine; (c) contacting said modified nucleic acid in a single stranded form with a sequencing primer hybridizeable with a stretch of nucleotides of said single stranded form of said modified nucleic acid; (d) synthesizing a complementary nucleic acid being complementary to said single stranded form of said modified nucleic acid, said synthesizing being carried out in a stepwise serial manner in which the identity of each nucleotide incorporated into said complementary nucleic acid is determined subsequent to its incorporation; and (e) determining a sequence of said single stranded form of said modified nucleic acid, thereby characterizing said GC rich region of the nucleic acid of interest.