Patent ID: 6190873
Filing Date: 2001-02-20
Classification: C07K,G01N,Y02A,Y10S

Abstract:
An assay for the presence of cyclosporin in a sample suspected of containing cyclosporin, wherein the assay comprises:(A) combining in an aqueous medium:1) said sample suspected of containing cyclosporin,2) a cyclosporin-label conjugate comprising cyclosporin conjugated at an alanine nitrogen atom of amino acid residue 7 or 8 to a label, and3) antibodies capable of binding to said cyclosporin-label conjugate, wherein said antibodies have been raised against a cyclosporin-immunogenic carrier conjugate comprising cyclosporin conjugated to an immunogenic carrier,(B) determining the presence of a detectable complex; and(C) correlating said detectable complex with the presence of cyclosporin in said sample;wherein said cyclosporin is conjugated to said label and said immunogenic carrier through a first and a second linking group, respectively;wherein said linking groups are independently selected from the group consisting of(I) a hydroxyalkane chain of the formula --(CH.sub.2).sub.n O--, where n is an integer of about 1 to 6;(II) a carboxyl benzyl group of the formula: ##STR20##wherein an alanine nitrogen atom of cyclosporin is conjugated to the benzylic carbon atom in the carboxyl benzyl group; and(III) a moiety of the formula: ##STR21## ##STR22## ##STR23##