Patent ID: 7150978
Filing Date: 2006-12-19
Classification: C07K,C12P

Abstract:
1. A method for producing a C-terminal modified protein, which comprises expressing a translation template in a cell free protein synthesis system or a cell expression system in the presence of a modifying agent to obtain protein synthesis, and purifying the synthesized protein, wherein the translation template comprises an ORF region coding for a protein, a 5′ untranslated region located on the 5′ side of the ORF region comprising a transcription promoter and a translation enhancer and a region comprising a poly-A sequence located on the 3′ side of the ORF region, and wherein the modifying agent comprises an acceptor portion having a group capable of binding to the synthesized protein through a transpeptidation reaction in a protein translation system and a modifying portion comprising a non-radioactive modifying substance linked to the acceptor portion via a nucleotide linker selected from the group consisting of 2′-deoxycytidylic acid, 2′-deoxycytidyl-(3′,5′)-2′-deoxycytidylic acid, and ribocytidyl-(3′,5′)-ribocytidylic acid, wherein the acceptor portion comprises a substance having a chemical structure of a member selected from the group consisting of puromycin, 3′-N-aminoacylpuromycin and 3′-N-aminoacyladenosine aminonucleoside.