Patent ID: 8338107
Filing Date: 2012-12-25
Classification: A61K,C07K

Abstract:
1. A method of modifying the binding affinity of an antibody to an antigen, said method comprising providing an antibody comprising a heavy chain variable domain and a first light chain variable domain, which binds to said antigen, providing a nucleic acid encoding said heavy chain variable domain of said antibody, recombining the nucleic acid encoding said heavy chain variable domain with a library of at least 10 3 different light chain variable domain encoding nucleic acids, expressing said recombined nucleic acids in a host cell, and selecting an antibody having a modified binding affinity to said antigen relative to the antibody comprising said first light chain variable domain, wherein said antibody having modified binding affinity is encoded by said heavy chain variable domain encoding nucleic acid recombined with a second light chain variable domain encoding nucleic acid.