Patent ID: 9051613
Filing Date: 2015-06-09
Classification: C12Q

Abstract:
1. A method for identifying an olfactory receptor included in one olfactory cell among a mouse olfactory receptor group A; the method comprising steps of: (a) obtaining the one olfactory cell; (b) extracting mRNA contained in the one olfactory cell obtained in the step (a); (c) obtaining cDNA with reverse transcriptase from the mRNA extracted in the step (b); (d) amplifying the cDNA obtained in the step (c) by a PCR method using a forward primer represented by SEQ ID: 01 and a reverse primer represented by SEQ ID: 02; wherein SEQ ID:01 is G (guanine) -C (cytosine) -I (inosine) -T (thymine) -A (adenine) -Y (thymine or cytosine) -G (guanine) -A (adenine) -Y (thymine or cytosine) -C (cytosine) -G (guanine) -I (inosine) -T (thymine) -A (adenine) -Y (thymine or cytosine) -G (guanine) -T (thymine) -I (inosine) -G (guanine) -C (cytosine) -I (inosine) -A (adenine) -T (thymine) -I (inosine) -T (thymine) -G (guanine); and SEQ ID: 02 is T (thymine) -G (guanine) -Y (thymine or cytosine) -Y (thymine or cytosine) -T (thymine) -G (guanine) -G (guanine) -T (thymine) -I (inosine) -Y (thymine or cytosine) -T (thymine) -I (inosine) -A (adenine) -T (thymine) -I (inosine) -C (cytosine) -Y (thymine or cytosine) -R (guanine or adenine) -T (thymine) -A (adenine) -I (inosine) -A (adenine) -T (thymine) -I (inosine) -A (adenine) -T (thymine) -I (inosine) -G (guanine) -G (guanine) -R (guanine or adenine) -T (thymine) -T (thymine); (e) determining whether or not a gene sequence of the cDNA amplified in the step (d) corresponds with one gene sequence included in gene sequences coding for olfactory receptors included in the mouse olfactory receptor group A; wherein the mouse olfactory receptor group A includes the following mouse olfactory receptors: (Mouse olfactory receptor group A) Olfr17, Olfr30, Olfr64, Olfr122, Olfr155, Olfr156, Olfr159, Olfr291, Olfr510, Olfr544, Olfr545, Olfr547, Olfr548-ps1, Olfr566, Olfr569, Olfr578, Olfr582, Olfr583, Olfr584, Olfr619, Olfr630, Olfr665, Olfr676, Olfr702, and Olfr713.