Patent ID: 6699677
Filing Date: 2004-03-02
Classification: A61P,C07K,G01N

Abstract:
A method for identifying an interaction between an orphan receptor and a ligand, said method comprising:(a) contacting a cell expressing the orphan receptor or a ligand-binding portion thereof with an array of different tethered ligand fusion proteins immobilized to a substrate, wherein each tethered ligand fusion protein comprises a ligand domain and a stalk domain, different tethered ligand fusion proteins have different ligand domains and are located at different locations on the substrate; (b) detecting binding of said cell at a location on the array; and (c) identifying the ligand for the orphan receptor from the identity of the ligand domain of the fusion protein at the binding location, wherein the ligand comprises the ligand domain of the fusion protein at the binding location.