Patent ID: 7563602
Filing Date: 2009-07-21
Classification: C12N,C12P

Abstract:
1. A process for producing an L-amino acid comprising: culturing an isolated strain of coryneform bacteria in a suitable medium to produce a fermentation liquor, and recovering the amino acid from the fermentation liquor; wherein said isolated strain of coryneform bacterium was obtained by: a) treating a coryneform bacterium that has the ability to secrete amino acids with a mutagenic agent, b) isolating and replicating the mutant bacterium produced in a), c) preparing a nucleic acid from the mutant obtained in b), d) producing a nucleic acid molecule using the polymerase chain reaction, the nucleic acid from c), and a primer pair consisting of a first primer comprising at least 15 successive nucleotides selected from the nucleotide sequence between position 1 and 1210 of SEQ ID NO: 3 or SEQ ID NO: 19 and a second primer comprising at least 15 successive nucleotides selected from the complementary nucleotide sequence between position 1866 and 1214 of SEQ ID NO: 3 or SEQ ID NO: 19, e) determining the nucleotide sequence of the nucleic acid molecule obtained in d), and determining the amino acid sequence coded for, f) optionally, comparing the amino acid sequence determined in e) with SEQ ID NO: 6 or 18, and g) identifying a mutant which contains a polynucleotide that codes for a polypeptide which contains at position corresponding to position 329 of SEQ ID NO: 2 any proteinogenic amino acid other than L-valine.