Patent ID: 6258537
Filing Date: 2001-07-10
Classification: C12Q

Abstract:
A method for objectively detecting Didymella bryoniae infection in a whole plant, plant extract, or plant seed by using cycles of a polymerase chain reaction, said method comprising the steps of:(a) removing a sample of leaf, stem, or seed from a plant to be tested fo gummy stem blight;(b) extracting genomic DNA from said sample for use as a DNA template;(c) replicating a desired region in the DNA template by binding a specific oligonucleotide primer set of SEQ ID:11 and SEQ ID:12 to said DNA template in a complementary fashion, said primer set designed to give rise to a desired PCR product having a size different from the size of a product produced from amplifying undesired genomic DNA;(d) amplifying complementary DNA by conducting the polymerase chain reaction from about 25 cycles to about 35 cycles to produce a PCR product; and(e) examining said PCR product to identify Didymella bryoniae present in said sample.