Patent ID: 8778617
Filing Date: 2014-07-15
Classification: B01D,B01L,G01N

Abstract:
1. A method for determining the binding constant of a high affinity compound of interest to proteins comprising the following steps: a) adding the high affinity compound to a two-chamber system, wherein the two chambers are separated by a semipermeable membrane, which is permeable for the high affinity compound of interest, and b) adding a sink compound wherein the sink compound is polyvinylpyrrolidone(PVP) or cyclodextrin to either one of the chambers whereby the sink compound can not permeate the membrane, and c) adding a plasma protein to the other chamber used in step b) wherein the high affinity compound of interest and the sink compound have been added, whereby the plasma protein can not permeate the membrane, and d) determining the binding constant of the high affinity compound of interest with the distribution coefficient of steps b) and c).