Patent ID: 6866827
Filing Date: 2005-03-15
Classification: B01J,C07H,C40B,Y02P

Abstract:
1. A process for the parallel synthesis of at least 4n oligonucleotides, with the steps: arrangement of at least four inserts each with n reaction vessels on a plate such that a first insert is at a first station, a second insert is at a second station, a third insert is at a third station and a fourth insert is at a fourth station, each reaction vessel containing an initiator base bound to an inert carrier, or a universal carrier, carrying out in parallel of a) a deblocking operation to remove protective groups simultaneously in all n reaction vessels of the insert at the first station, b) a first washing operation simultaneously in all n reaction vessels of the insert at the second station, c) a coupling operation for attaching individual nucleotides in all n reaction vessels of the insert at the third station, and d) a second washing operation followed by a capping operation for blocking oligonucleotides which have not undergone the desired chain lengthening in the preceding coupling operation followed by a third washing operation followed by an oxidation operation for stabilizing the phosphate foundation matrix of the oligonucleotides, followed by a fourth washing operation simultaneously in all n reaction vessels of the insert at the fourth station, and station by station rotation of the inserts through the four stations mentioned or of the stations relative to the inserts and carrying out of the above-mentioned operations until the desired oligonucleotides have been formed by coupling of the individual nucleotides to one another.