Patent ID: 6319714
Filing Date: 2001-11-20
Classification: A61K,B01J,C07K,C12N

Abstract:
A method of recombining two or more nucleic acids, the method comprising:(i.) aligning two or more nucleic acid sequence strings representing the two or more nucleic acids to identify regions of identity and regions of diversity;(ii.) providing a non-equimolar set of selected oligonucleotides, which set of selected oligonucleotides comprises a plurality of oligonucleotides which correspond in sequence to at least two of the two or more nucleic acids at at least one region of diversity, the oligonucleotides being present in non-equimolar amounts; and,(iii.) extending the oligonucleotides with a polymerase, thereby producing a plurality of recombinant nucleic acids.