Patent ID: 6699687
Filing Date: 2004-03-02
Classification: C07K

Abstract:
An isolated nucleic acid sequence encoding a circularly permuted Aequorea-related fluorescent protein moiety which provides measurable fluorescence in a fluorescence screening assay, comprising:a linker moiety linking the amino-termninal and carboxy-terminal amino acids of a fluorescent protein, wherein the amino and carboxy termini are linked as internal amino acids in the circularly permuted fluorescent protein moiety; and two terminal ends, wherein the first end is an amino-terminal end and the second end is a carboxy terminal end, wherein the amino and carboxy terminal ends of the circularly permuted fluorescent protein moiety are different from the amino-terminal and carboxy-terminal amino acids of the fluorescent protein, wherein amino acid residues of said fluorescent protein are numbered in conformance with native Aequorea green fluorescent protein (SEQ ID NO:3) and wherein the first serine residue following methionine-1 in said fluorescent protein is designated amino acid residue 2, and wherein the amino-terminal end is selected from the group consisting E142, Y143, Y145, H148, D155, H169, E172, D173, A227 and I229, and the carboxy-terminal end is selected from the group consisting of N144, N146, N144, N149, K162, K156, N170, I171, D173, E172, A227, and I229, of the fluorescent protein, respectively.