Patent ID: 7999073
Filing Date: 2011-08-16
Classification: A61K,C07K,C12N

Abstract:
1. A transfection agent for transfecting cells with a nucleic acid, comprising at least one protein being capable of forming nucleoprotein filaments (NPFs), said nucleic acid and said NPF-forming protein forming a nucleoprotein filament complex, wherein said NPF-forming protein is a protein selected from the group consisting of the proteins RadA, hDmc1, SASP, ICP8, and UvsX, or a mixture of at least 2 of the listed proteins, wherein said NPF-forming protein is modified by at least one modification selected from the group consisting of deletion or insertion of amino acids and/or protein domains, chemical alteration of amino acids, and chemical coupling of peptides, proteins, carbohydrates or lipids, wherein said NPF-forming protein is modified with a single functional component or several functional components of the same function, wherein said functional component(s) cause(s) association of said complex to the cellular surface, non-endosomal passage of said complex through the cell membrane, release of said complex from endosomes/lysosomes, or transport of said complex into the nucleus, and wherein said functional component(s) is/(are) selected from the group consisting of amino acid linker, Nuclear Localization Signal (NLS), ligand to the Epstein Barr Virus receptor CD21, ligand to