Patent ID: 6815539
Filing Date: 2004-11-09
Classification: C07K,G01N

Abstract:
An isolated nucleic acid encoding a polypeptide monomer of a pH sensitive potassium channel, the monomer:(i) forming a potassium channel having a unit conductance of approximately 80-120 pS and having increased potassium channel current activity above approximately intracellular pH of 7.1, when the monomer is expressed in a Xenopus oocyte; and (ii) encoded by a nucleic acid that selectively hybridizes under highly stringent hybridization conditions to a nucleic acid comprising a nucleotide sequence of SEQ ID NO:2, SEQ ID NO:17, or SEQ ID NO:19, wherein the hybridization reaction is incubated at 42Â° C. in a solution comprising 50% formamide, 5Ã—SSC, and 1% SDS and washed at 65Â° C. in a solution comprising 0.2Ã—SSC and 0.1% SDS.