Patent ID: 6184036
Filing Date: 2001-02-06
Classification: C12N

Abstract:
An isolated nucleic acid fragment comprising a member selected from the group consisting of:(a) an isolated nucleic acid fragment encoding an orthinine-oxo-acid transaminase that is at least 500 contiguous nucleotides that hybridizes under highly stringent conditions to an isolated nucleic acid fragment encoding the amino acid sequence set forth in SEQ ID NO:2, wherein said highly stringent conditions is defined by washes with 6.times. SSC, 0.5% SDS at room temperature for 15 min, then repeated with 2.times. SSC, 0.5% SDS at 45.degree. C. for 30 min, and two washes in 0.1.times. SSC, 0.1% SDS at 65.degree. C.;(b) an isolated nucleic acid fragment encoding an orthinine-oxo-acid transaminase that is at least 250 nucleotides that hybridizes under highly stringent conditions to an isolated nucleic acid fragment encoding the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, and SEQ ID NO:10, wherein said highly stringent conditions is defined in (a); and(c) an isolated nucleic acid fragment that is fully complementary to the nucleic acid fragment of (a) or (b).