Patent ID: 6777198
Filing Date: 2004-08-17
Classification: G01N,Y10S

Abstract:
A method of determining the presence of an analyte in a sample comprising the steps of:a) contacting the sample with a known amount of a receptor which binds specifically to the analyte to form an analyte/receptor complex, wherein the known amount of receptor is in excess of an amount of the receptor required to bind all analyte in the sample, b) isolating on a solid phase a fraction of the receptor contacted with the analyte, the resulting isolated fraction of receptor contacted with analyte including the analyte/receptor complex and unreacted receptor, and the ratio between the receptor in said isolated fraction and the known amount of receptor contacted with the sample being in a range of from about 1:2 to about 1:1000, c) labeling the analyte/receptor complex in said isolated fraction and detecting the amount of labeled analyte/receptor complex in said isolated fraction; and d) from the detected amount of labeled analyte/receptor complex, determining the presence of analyte in the sample.