Patent ID: 6790647
Filing Date: 2004-09-14
Classification: C12N,C12P

Abstract:
A mutant of N-acetylglutamate synthase wherein amino acid residues corresponding to positions 15 to 19 in the N-acetylglutamate synthase of SEQ ID NO:16 are replaced with any one of amino acid sequences of SEQ ID NOs: 1 to 4, and feedback inhibition by L-arginine is desensitized,wherein the N-acetylglutamate synthase is a protein defined in the following (A) or(B): (A) a protein having the amino acid sequence defined in SEQ ID NO: 16; or (B) a protein that is encoded by a DNA which hybridizes with a DNA having the nucleotide sequence defined in SEQ ID NO: 15 under stringent conditions, wherein said stringent conditions entail a temperature ranging from 60Â° C. to 65Â° C., a salt concentration ranging from 0.1Ã—SSC to 1Ã—SSC, and 0.1% SDS, and wherein said mutant has a N-acetylglutamate synthase activity.