Patent ID: 7869959
Filing Date: 2011-01-11
Classification: C12Q,G16B

Abstract:
1. A method for optimizing oligonucleotide probes for use in a hybridization-based assay, the method comprising the steps of: a) providing a plurality of oligonucleotide probes on a hybridization array; b) providing serial dilutions of a genomic sample, wherein the genomic sample is labeled; c) hybridizing the labeled and serially diluted genomic sample to the probes on the array, such that a signal intensity is produced for each of the probes, wherein the hybridization step is performed at least one time; d) computationally generating weighted regression data from the signal intensity produced for each of the probes; and e) identifying optimized probes from the plurality of oligonucleotide probes on the hybridization array for use in the assay using a probe selection algorithm reliant upon the weighted regression data, the optimized probes exhibiting signal intensities proportional to the serial dilutions of the genomic sample and having maximal brightness at each serial dilution.