Patent ID: 7741285
Filing Date: 2010-06-22
Classification: A61K,C07K

Abstract:
1. A method of recovering Apo2L/TRAIL from a mixture comprising (a) loading the mixture on a cation exchange column; (b) washing the cation exchange column with an equilibration buffer whereby non-binding components present in the mixture are removed; (c) eluting Apo2L/TRAIL bound to the cation exchange column with an elution buffer; (d) crystallizing Apo2L/TRAIL from the eluate obtained in step (c), wherein said crystallization consists of gradually cooling the eluate to a temperature of about 2 to 8° C., in the presence or absence of an anti-solvent, whereby Apo2L/TRAIL is spontaneously precipitated in a crystalline form to yield a mixture of mother liquor and Apo2L/TRAIL crystals, and (e) recovering Apo2L/TRAIL from the mixture obtained in step (d) in a purity of at least about 99%.