Patent ID: 6444871
Filing Date: 2002-09-03
Classification: A01K,A61K,A61P,C07K,C12N

Abstract:
A method for recombinantly producing a polypeptide in a mouse cell that makes the tet repressor protein, said method comprising:a) tranforming a mouse embryonic stem cell with a vector comprising: i) a mammalian promoter sequence having a TATA element; wherein said promoter is operable in adult mouse tissues. ii) at least one tet operator sequence positioned at least 6 nucleotides 3â€² to the TATA element; and iii) a nucleic acid encoding said polypeptide lying 3â€² to said tet operator and operably linked to said promoter; wherein said mouse embryonic stem cell comprises the tet repressor protein or is transformed with a vector that expresses the tet repressor protein; b) selecting a mouse embryonic stem cell that expresses said polypeptide; c) incorporating said mouse embryonic stem cell into a blastoyst to form a chimeric mouse embryo; d) implanting said chimeric mouse embryo into a pseudopregnant mouse; e) allowing said chimeric mouse embryo to develop into viable offspring; f) screening said viable offspring to identify heterozygous transgenic mice expressing said polypeptide; g) breeding said heterozygous transgenic mice to produce homozygous transgenic mice; and h) administering tetracycline to said homozygous transgenic mice to produce said polypeptide.