Patent ID: 6812341
Filing Date: 2004-11-02
Classification: C12N

Abstract:
A method for purifying poly(A) mRNA from a sample in a manner that reduces rRNA carryover comprising:a) incubating a composition comprising: i) the sample, wherein the sample includes poly(A) mRNA; ii) a poly(dT) or poly(U) nucleic acid molecule; and iii) tetramethylammonium chloride (TMAC) or tetraethylammonium chloride (TEAC), under conditions allowing poly(A) mRNA to hybridize with the poly(dT) or poly(U) nucleic acid molecule and inhibiting partial hybridization of the poly(A) mRNA to any rRNA that may be present in the sample; and b) isolating the poly(dT) or poly(U) nucleic acid molecule and the hybridized poly(A) mRNA; wherein rRNA carryover is reduced.