Patent ID: 7786290
Filing Date: 2010-08-31
Classification: A61P,C12N

Abstract:
1. A double-stranded ribonucleic acid (dsRNA), consisting of first and second single RNA strands, wherein the first single RNA strand is an antisense RNA strand, the second single RNA strand is a sense RNA strand, wherein the antisense RNA strand is complementary to a target gene or a portion thereof, the dsRNA having increased effectiveness in inhibiting the expression of a target gene by means of RNA interference, wherein the dsRNA comprises first and second double-stranded ends and at least one single-stranded overhang which is 2 to 4 nucleotides in length, wherein the unpaired nucleotide of the single-stranded overhang that is directly adjacent to the terminal nucleotide base pair comprises a purine base, wherein a single-stranded overhang is located at the 3′-end of the antisense strand, wherein the overhang comprises the sequence 5′-GC-3′; and wherein the terminal base pair of the first double-stranded end comprises a guanine-cytosine (G-C) base pair or the four consecutive terminal base pairs of the first double-stranded end comprises at least two G-C base pairs; wherein the terminal base pair of the second double-stranded end comprises a guanine-cytosine (G-C) base pair or the four consecutive terminal base pairs of the second double-stranded end comprises at least two G-C base pairs; wherein the region of the antisense strand that is complementary to the targer gene is 19-28 nucleotides in length excluding the following dsRNAs: