Patent ID: 7709192
Filing Date: 2010-05-04
Classification: C07B,C12Q,C40B,Y10S

Abstract:
1. A method for detecting the presence of a plurality of different target molecules suspected of being contained in a test mixture, wherein said target molecules to be detected are proteins, the method comprising: a) providing a biochip comprising a solid support, said solid support comprising a plurality of spatially defined addresses, each said address comprising nucleic acid ligand attached thereto, each said nucleic acid ligand having specific binding affinity for one of said target molecules suspected of being contained in said test mixture, and said nucleic acid ligand being non-naturally occurring and binding specifically to said target molecule through non-Watson-Crick interactions, and not having the known physiological function of binding to the target protein; b) contacting said biochip with said test mixture suspected of containing said target molecules; c) removing non-specifically bound material from said biochip; d) contacting said biochip with a reagent that is at least 8,000 fold more reactive on a mole:mole basis covalently and non-specifically with proteins than with nucleic acids, wherein said proteins are bound specifically to the nucleic acid ligands on the surface of said biochip; and e) detecting the presence of said target molecules by detecting the presence of said reagent at the appropriate address on said biochip.