Patent ID: 9212397
Filing Date: 2015-12-15
Classification: C12Q

Abstract:
1. A method for the in vitro amplification and detection of a nucleic acid in a sample, said nucleic acid being from one or more species in the class Mollicutes, comprising the steps of: (a.) contacting a sample with at least four tagged amplification oligomers and at least one additional amplification oligomer for generating amplification product from one or more nucleic acids in said sample, wherein each of said at least four tagged amplification oligomers, designated (i)-(iv), individually comprises a target hybridizing region and a tag region, wherein said at least four tagged amplification oligomers are selected as follows: and wherein the at least one additional amplification oligomer includes a promoter-based amplification oligomer comprising a target hybridizing region having a nucleotide sequence that is SEQ ID NO:42; (b.) performing an in vitro amplification reaction under suitable conditions to generate an initial amplification product from said one or more target nucleic acids using said at least two tagged amplification oligomers and said at least one additional amplification oligomer; and (c.) detecting the initial amplification product generated in step (b.), wherein said detecting comprises contacting the initial amplification product with a detection probe having a nucleotide sequence that is SEQ ID NO:14.