Patent ID: 7794976
Filing Date: 2010-09-14
Classification: C07K,C12N

Abstract:
1. A method of producing a recombinant antibody or antigen-binding fragment thereof, the method comprising contacting human embryonic kidney cells in a medium with a first vector and a second vector, wherein (a) the first vector encodes a heavy chain of an immunoglobulin, or a functional fragment thereof and the second vector encodes a light chain of the immunoglobulin, or a functional fragment thereof, wherein the functional fragments thereof are capable of forming an antigen-binding fragment of the immunoglobulin, (b) each of the first vector and the second vector comprises a 3′ untranslated region (UTR) of a light chain gene and an oriP, wherein the 3′UTR comprises a nucleotide sequence of nucleotides 1062-2560 of SEQ ID NO: 1, (c) the heavy chain or functional fragment thereof encoded by the first vector and the light chain or functional fragment thereof encoded by the second vector are each expressed from a promoter that is the same for each of the first and second vectors, and (d) the second vector is present in the medium in an amount which is about 1.5 to 2.5 times as much as the amount of the first vector, whereupon a recombinant antibody or antigen-binding fragment thereof is produced.