Patent ID: 9017938
Filing Date: 2015-04-28
Classification: A61P,C12N,C12Q

Abstract:
1. A diagnostic kit in vitro for assessing a histopathological stage of tumour and presence of metastases, wherein the assessing is performed by detecting in a biological sample expression of a sequence comprising SEQ ID NO: 1 through PCR Real-time and detecting one or more genes express in cancer stem cells chosen among CD133, c-myc, Nanog, Oct4, TCFL1, ZIC1, KLF5, HMGA1, HMGB3, wherein the kit comprises: a) a pair of amplification primers for detecting the sequence comprising SEQ ID NO:1, including: b) a pair of control primers for normalizing an expression value of the sequence comprising SEQ ID NO: 1; c) one or more of a pair of primers together with a probe for detecting the one or more genes, including: d) a pair of primers and a probe for normalizing an expression value of the genes in c), including: