Patent ID: 7893035
Filing Date: 2011-02-22
Classification: A61P,C12N,C12Y

Abstract:
1. A multicistronic short interfering RNA expressing construct comprising at least a first and a second self-complementary inverted complement repeat sequence separated by an intervening sequence of about 22-68 base pairs in length, wherein the construct is used to suppress angiogenesis, inhibit tumor formation and to regress pre-formed tumors, wherein the first inverted complement repeat sequence comprises a nucleotide sequence of urokinase-type plasminogen activator receptor (uPAR) and the second inverted complement repeat sequence comprises a nucleotide sequence of matrix metalloprotease 9 (MMP-9) and wherein the self complementary sequence of uPAR is CTACAGCAGTGGAGAGCGATT-loop-AATCGCTCTCCACTGCTGTAG (SEQ ID NO: 2) and the self complementary sequence of MMP-9 is CAAGTGGCACCACCACAACAA-loop-TTGTTGTGGTGGTGCCACTTG (SEQ ID NO: 4).