Patent ID: 9109015
Filing Date: 2015-08-18
Classification: C07K

Abstract:
1. A method of purifying a composition containing a recombinant protein and an impurity, the method comprising: (a) lowering the pH of the composition to a pH range of about 3.0 to about 6.5; (b) adding a divalent cation selected from the group consisting of Co (c) separating the protein from the impurity, wherein the protein comprises an antibody or a fusion protein comprising an Fc region, which is produced by a recombinant eukaryotic host cell, wherein the composition comprises a harvest feed of the recombinant eukaryotic host cell, wherein the addition of the divalent cation increases the recovery of the protein by greater than 3% compared to the amount of protein recovered without the addition of the divalent cation, and wherein the separating of (c) is performed by subjecting the composition to centrifugation, the centrifugation forming a supernatant and a precipitate wherein the protein is substantially in the supernatant, or wherein the separating of (c) is performed by filtering the composition, the filtering forming a permeate stream and a retentate stream wherein the protein is substantially in the permeate stream, and wherein (a) and (b) are carried out sequentially in any order before (c).