Patent ID: 7544486
Filing Date: 2009-06-09
Classification: A61P,C07K

Abstract:
1. A method of expressing a functional peptide in a mammalian cell, said method comprising: providing a nucleic acid construct including at least a nucleic acid encoding at least a NELL 1 peptide in frame with a nucleic acid encoding a secretory signal peptide; transfecting a mammalian cell with said nucleic acid construct; culturing said mammalian cell under conditions that permit expression of the NELL 1 peptide; optionally collecting NELL 1 peptide secreted from the cell line; optionally substantially purifying the NELL 1 peptide; and optionally testing the activity of the NELL 1 peptide to induce bone formation, wherein the secretory signal peptide is selected from the group consisting of a melittin signal sequence, a drosphila immunoglobulin-binding protein signal sequence, an equine interferon-gamma (elFN-gamma) signal peptide, a snake phospholipase A2 inhibitor signal peptide, a human lysozyme signal peptide, and a chicken lyzozyme signal peptide.