Patent ID: 8969038
Filing Date: 2015-03-03
Classification: C07K,C12N

Abstract:
1. A recombinant gram-negative bacterial cell, wherein the cell: a) comprises an exogenous recombinant polynucleotide encoding DsbC; and b) has reduced Tsp protein activity as compared to a wild-type cell resulting from a mutation in the Tsp gene that encodes a mutated Tsp protein having reduced protease activity or a mutation in the Tsp gene or regulatory sequence of the Tsp gene that reduces or eliminates expression of the Tsp protein, and wherein the cell's genome is isogenic to a wild-type bacterial cell except for: the mutation in the Tsp gene, the presence of the recombinant polynucleotide encoding DsbC and optionally, one or more of: a) a mutant spr gene encoding a mutant spr protein containing a mutation that is an amino acid substitution at one or more amino acids within the spr protein, wherein the mutant spr protein reduces lysis of cells containing a mutated Tsp gene; b) a mutated DegP gene encoding a DegP protein having chaperone activity and reduced protease activity; c) a mutated ptr gene, wherein the mutated ptr gene encodes a Protease III protein having reduced protease activity or is a knockout mutated ptr d) a mutated OmpT gene, wherein the mutated OmpT gene encodes a OmpT protein having reduced protease activity or is a knockout mutated OmpT gene; and e) a polynucleotide sequence encoding a protein of interest.