Patent ID: 7112411
Filing Date: 2006-09-26
Classification: C12Q,G01N

Abstract:
1. In a method for detecting a target biomolecule directly in a polyacrylamide gel comprising (1) separating the target biomolecule from other biomolecules by electrophoresis in the gel and, while the target biomolecule remains in the gel, (2) associating the target biomolecule with an antibody having a label which is capable of providing a detectable signal, (3) removing any non-associated antibody from the gel, and (4) detecting the presence of the target biomolecule by observing a signal attributable to the presence of the labeled antibody associated with the target biomolecule; the improvement wherein, prior to associating the target biomolecule with the antibody and while the target biomolecule remains separated in the gel, (A) shrinking the gel by at least about 10% and then (B) washing the gel with water for a sufficient time to restore the gel to substantially its original size.