Patent ID: 9170197
Filing Date: 2015-10-27
Classification: C12Q,G01N

Abstract:
1. An assay system for isolating a target biological molecule, the system comprising: a testing sample suspected of containing a DNA sequence of the target biological molecule, wherein the DNA sequence of the target biological molecule is known; a substrate consisting of a layer of immobilized metal particles positioned on the substrate, wherein the immobilized metal particles have attached thereto an immobilized capture nucleotide sequence probe complementary to a first known nucleotide sequence of a specific nucleotide sequence of the target biological molecule, wherein the immobilized capture nucleotide sequence probe is covalently linked to the immobilized metal particles, wherein each immobilized capture nucleotide sequence probe is the same and having a specific length and sequence of nucleotides and any DNA sequence of the target biological molecule in the testing sample binds to the capture nucleotide sequence probe; and a multiplicity of free nucleotide sequence probes, wherein each of the free nucleotide sequence probes is the same having a specific length and sequence of nucleotides, wherein each of the free nucleotide sequence probes is complementary to a second known nucleotide sequence of the specific nucleotide sequence of the target biological molecule, wherein the second known nucleotide sequence is different from the first known nucleotide sequence of a specific nucleotide sequence of the target biological molecule, wherein each of the free nucleotide sequence probe has attached thereto a fluorophore, wherein the fluorophore is incorporated at a specific location on the free nucleotide sequence probe so that binding of the free nucleotide sequence probe to the nucleotide sequence of the target biological molecule causes the fluorophore to be positioned from about 50 to about 500 {acute over (Å)} from the immobilized metal particles.