Patent ID: 6692942
Filing Date: 2004-02-17
Classification: C07K,C12N

Abstract:
A polynucleotide encoding a single-chain antigen-binding polypeptide capable of delivering nucleic acids to a cell, said single-chain antigen-binding polypeptide comprising:(a) a first polypeptide comprising the antigen binding portion of the variable region of an antibody heavy or light chain; (b) a second polypeptide comprising the antigen binding portion of the variable region of an antibody heavy or light chain; and (c) a peptide linker linking the first and second polypeptides (a) and (b) into a single chain polypeptide having an antigen binding site, and wherein at its C-terminus, N-terminus, or both, the single-chain antigen-binding polypeptide has an amount of basic amino acid residues sufficient to bind nucleic acids, wherein the basic amino acid residues are selected from the group consisting of: oligo-Lys, oligo-Arg, and a combination thereof; and wherein the basic amino acid residues bind nucleic acid and wherein the single-chain antigen-binding polypeptide binds antigen.