Patent ID: 7919305
Filing Date: 2011-04-05
Classification: B01L,C12M,C12N

Abstract:
1. A method for producing a cell culture substrate, comprising: preparing a patterning substrate comprising a base material and a cell culture patterning layer formed on the base material and containing a cell adhesion-inhibiting material, preparing a photocatalyst-containing substrate comprising a base body and a photocatalyst-containing layer formed on the base body and containing a photocatalyst; arranging the cell culture patterning layer of the patterning substrate and the photocatalyst-containing layer of the photocatalyst-containing substrate to face each other so that the cell culture patterning layer and the photocatalyst-containing layer contact each other or face at a given interval; forming a pattern on the cell culture patterning layer composed of a cell adhesion-inhibiting portion wherein no photocatalyst action has been affected and a cell adhesion portion wherein the cell adhesion inhibiting material is decomposed or denatured by action of the photocatalyst, by irradiating with energy a region of the photocatalyst-containing layer facing a region of the cell culture patterning layer to which the cell adhesion portion is desired to be formed, and by affecting the action of the photocatalyst contained in the region of the photocatalyst-containing layer irradiated with energy to the facing cell culture patterning layer; and applying a cell-containing liquid onto the cell adhesion portion by a region-selecting applying method whereby the cell-containing liquid is selectively applied only onto the cell adhesion portion.