Patent ID: 9005931
Filing Date: 2015-04-14
Classification: B01L,C12Q,Y02A

Abstract:
1. A quantitative method for analyzing target nucleic acids, comprising obtaining a substrate with three or more groups of different target nucleic acid probes immobilized in independent lines on an upper surface of the substrate, wherein each group has three or more probes, the probes within each group are different from probes in other groups, and the probe groups are arrayed on the substrate in order of decreasing hybridization temperature, but probes within each line have optimal hybridization temperatures within five percent of each other; annealing one or more fluorescently tagged target amplicons to target nucleic acid probes; independently and sequentially activating a fluorescence response from fluorescently tagged target amplicons hybridized to each line of target nucleic acid probes at the hybridization temperature of each group; independently and sequentially detecting each fluorescence response for a quantitative analysis of one or more target nucleic acids at the hybridization temperature of each line of target nucleic acid probes; wherein the activating of the each fluorescence response is by using an evanescent wave of a predetermined wavelength.