Patent ID: 7781211
Filing Date: 2010-08-24
Classification: C12N

Abstract:
1. A method of obtaining a homogeneous population of single cell-derived clonal multipotent bone marrow cells based on cell density from a biological sample of bone marrow, comprising: (i) allowing the biological sample to settle by gravity in a first container producing a first supernatant of lower density cells; (ii) transferring the first supernatant directly without undergoing centrifugation to a second container of growth medium and allowing cells to settle to the bottom producing a second supernatant of lower density cells; (iii) transferring the second supernatant directly without undergoing centrifugation to a third container of growth medium and allowing cells to settle to the bottom, producing a third supernatant of lower density cells; (iv) transferring the third supernatant directly without undergoing centrifugation to another container of growth medium and allowing cells to settle to the bottom, producing another supernatant of lower density cells; (v) allowing single-cell derived colonies to appear on the bottom of the container of step (iv); (vi) isolating the single-cell derived colonies; and (vii) expanding cells from the single-cell derived colonies in a further other container of growth medium to obtain the homogeneous population of single cell-derived clonal multipotent bone marrow cells.