Patent ID: 7364873
Filing Date: 2008-04-29
Classification: C12Q,Y10S

Abstract:
1. A method for preparing a lactate biosensing strip comprising an electrically insulated base support ( 1 ), a pair of isolated first and second silver layers deposited thereon ( 2 ), a pair of graphite layers, each one of said pair of graphite layers being deposited on one respective silver layer and electrically connected to said respective silver layer ( 2 ), the first silver layer being covered fully by the respective graphite layer, the second silver layer being covered partly in the middle thereof with the respective graphite layer after leaving the terminal and working zone uncovered, a Ag/AgCl electrode ( 4 ) provided on top of the working zone of said second silver electrode layer, lactate oxidase deposited with an electron mediator on the working zone of graphite layer covering the first silver layer ( 5 ), the silver/silver chloride electrode ( 4 ) and enzyme with mediator layer working electrode ( 5 ) being supported on said support ( 1 ), the working zone of silver/silver chloride reference electrode ( 4 ) and working electrode ( 5 ) being covered with a hydrophilic membrane, said lactate biosensing strip being disposable, said method comprising: (a) depositing a pair of silver layers on an electrically insulated base support; (b) depositing two graphite layers on each of said two silver layers, each of said two silver layers being deposited with one graphite layer, a first graphite layer completely covering the first silver layer, and the second graphite layer covering the second silver layer only in part of the surface thereof that is away from the surface facing the base support; (c) depositing a silver chloride layer on the second silver layer on the part thereof that is not deposited with a graphite layer to obtain a silver/silver chloride electrode; (d) adsorbing physically lactate oxidase enzyme with an electron mediator on the first graphite deposited silver layer to obtain a working electrode; and (e) applying an outer hydrophilic membrane on said first reference electrode and second working electrode to obtain a pair of electrodes on an electrically insulated base support in a single assembly.