Patent ID: 8420017
Filing Date: 2013-04-16
Classification: B01F,B01J,B01L

Abstract:
1. A mixing arrangement in an elastomeric microfluidic device, the mixing arrangement comprising: a) a reaction well having a single inlet from a flow channel; b) a first valve configured to open and close the flow channel at a position at or near the single inlet into the reaction well; c) a second valve configured to open and close the flow channel at a position further from the reaction well than the first valve, thereby defining a segment of the flow channel between the first and second valve; d) a source of a first fluid that is fluidically connected to said segment by way of a first input channel forming a junction with the segment between the first and second valve; and e) a source of a second fluid that is fluidically connected to said segment by way of a second input channel positioned behind the second valve; wherein said segment of the flow channel is configured to receive a volume of the first fluid into the segment by blind filling from the first input channel when the first and second valves are closed; and wherein said reaction well is configured to receive said volume of the first fluid followed by a volume of the second fluid from said segment when the first and second valves are open.