Patent ID: 9012719
Filing Date: 2015-04-21
Classification: C12N,C12Y

Abstract:
1. A method for expressing a multidomain enzyme in a plant cell comprising introducing into said plant cell a nucleic acid construct comprising a nucleotide sequence encoding a modified multidomain enzyme, wherein said multidomain enzyme comprises at least one first domain, at least one first linker sequence, and at least one second domain, wherein said first domain and said second domain are non-heterologous sequence, wherein the native linker sequence in said modified multidomain enzyme has been replaced with a heterologous linker sequence, wherein said heterologous linker sequence is a linker sequence that is not cleaved by a plant protease, wherein said heterologous linker sequence is selected from the group consisting of SEQ ID NO:18, 19, and 20, and wherein the multidomain enzyme is targeted to a vacuole.