Patent ID: 7608399
Filing Date: 2009-10-27
Classification: B01L,C12Q,Y10T

Abstract:
1. A method for determining the quantity of nucleic acids in a sample, comprising: (a) introducing a sample containing cells in a liquid medium into a first chamber of a vessel, the vessel comprising the first chamber and a second chamber, wherein the first chamber is in liquid communication with the second chamber and wherein the second chamber has at least a portion of one flat glass surface effective for binding nucleic acids; (b) lysing the cells to provide nucleic acids in the liquid medium; (c) transferring at least a portion of the liquid medium from the first chamber into the second chamber; (d) extracting the nucleic acids from the liquid medium by binding the nucleic acids to the flat glass surface of the second chamber effective for binding nucleic acids to provide isolated nucleic acids; (e) contacting the isolated nucleic acids with a fluorescent compound having a fluorescence intensity dependent on the concentration of nucleic acids; and (f) measuring the fluorescence of the fluorescent compound to determine the quantity of isolated nucleic acids.