Patent ID: 7118864
Filing Date: 2006-10-10
Classification: C12Q

Abstract:
1. A method of detecting the presence or absence of an analyte in a sample comprising: (a) contacting said sample with an amplifiable probe under conditions whereby said analyte, if present, binds said amplifiable probe to form a probe/analyte complex, said amplifiable probe comprising: (b) extending said at least one extendable signal-generating nucleic acid in a reaction mixture comprising said probe/analyte complex, wherein said extendable signal-generating nucleic acid serves as its own template, wherein said extension comprises two or more cycles of extension, and wherein said extendable signal-generating nucleic acid is extended in each of said two or more cycles; and (c) detecting the presence or absence of said extended signal-generating nucleic acid, wherein presence of said extended signal-generating nucleic acid correlates with the presence of said analyte.