Patent ID: 6046040
Filing Date: 2000-04-04
Classification: C12N,C12P

Abstract:
A method for producing glycoconjugate, which comprises the steps of:(i) binding a sugar residue to a side chain of a water-soluble polymer via a linker having a selectively cleavable linkage to give a primer, and bringing said primer into contact with an immobilized glycosyltransferase in the presence of a sugar nucleotide, to transfer a sugar residue of said sugar nucleotide to the sugar residue of said primer;(ii) elongating a sugar chain by transfer of a plurality of sugar residues by repeating step (i) at least once;(iii) optionally removing a by-produced nucleotide or an unreacted sugar nucleotide;(iv) optionally repeating steps (i)-(iii); and(v) optionally releasing the resultant glycoconjugate sugar chain by selectively cleaving the cleavable linkage in the linker from the primer, which connects the sugar chain elongated by the transfer of the plurality of sugar residues.