Patent ID: 8952152
Filing Date: 2015-02-10
Classification: A61P,C07D,C12N,C12P

Abstract:
1. A method for the isolation and purification of neosaxitoxin produced by cyanobacteria which produce neosaxitoxin and saxitoxin, the method comprising: (a) Lysing the cyanobacteria, (b) cold extracting the phycotoxins from the lysed cyanobacteria at pH 5 to obtain a concentrate, (c) passing the concentrate through a molecular weight separation column to obtain a phycotoxin eluate containing predominately neosaxitoxin, and (d) passing the eluate through a cation-exchange column, washing the column with acid and eluting the neosaxitoxin with an acidic alcoholic extraction solution to produce a highly pure neosaxitoxin eluant.