Patent ID: 8399188
Filing Date: 2013-03-19
Classification: C07H,C12P,C12Q

Abstract:
1. A method for sequencing a nucleic acid molecule, comprising: (a) contacting a nucleic acid template, a nucleic acid primer complementary to a portion of said template, an extension enzyme, and a reversibly blocked nucleotide under conditions wherein said primer is extended to incorporate said nucleotide into a sequence complementary to said nucleic acid template, said reversibly blocked nucleotide comprising a sugar moiety and a base moiety, wherein said base moiety is linked to a label by an enzymatically cleavable peptide linker, and wherein, attached at the 2′ or 4′ position of said sugar moiety, is a reversible blocking group comprising —CH (b) identifying the nucleotide in said sequence complementary to said nucleic acid template; (c1) enzymatically cleaving the peptide linker; and (c2) enzymatically removing the blocking group, wherein identifying said nucleotide identifies at least a portion of the sequence of said nucleic acid molecule.