Patent ID: 9085791
Filing Date: 2015-07-21
Classification: C07K,C12Q,C12Y,G01N

Abstract:
1. A polynucleotide encoding an amino acid sequence having: (1) a nonfluorescent peptide domain 1 of a fluorescent protein constituted of a nonfluorescent peptide domain 1 and a nonfluorescent peptide domain 2, characterized in that when nonfluorescent peptide domain 1 and nonfluorescent peptide domain 2 are associated, fluorescent activity is gained; and (2) an ATF6 protein domain, wherein the polynucleotide has a region encoding (1) at the 5′ end, a region encoding (2) at the 3′ end, and a region encoding a PEST sequences between the region encoding (1) and the region encoding (2), wherein the polynucleotide does not encode the fluorescent protein, and wherein the fluorescent protein is GFP, GFP mutants, or a GFP-like protein family member.