Patent ID: 8367318
Filing Date: 2013-02-05
Classification: A61P,C12N,C12Q

Abstract:
1. A method for screening a cell for at least one phenotype resulting from inhibition of a miRNA cluster, wherein said at least one phenotype is selected from the group consisting of increased cell death, decreased cell death, increased c-Jun nuclear translocation, decreased c-Jun nuclear translocation, increased NF-κB nuclear translocation, and decreased NF-κB nuclear translocation, and the miRNA cluster comprises miR-106b and at least one miRNA selected from the group consisting of miR-93 and miR-25, the method comprising: (a) introducing into said cell a cluster pool comprising at least one miRNA inhibitor specific for each miRNA in said miRNA cluster, wherein each miRNA inhibitor comprises a sequence that is at least partially the reverse complement of a mature miRNA sequence and flanking sequences located 5′ and 3′ to the sequence, wherein the flanking sequences are the reverse complements of sequences that are adjacent to the mature miRNA in a native pri-miRNA; and (b) determining whether said cell manifests said at least one phenotype.