Patent ID: 6372457
Filing Date: 2002-04-16
Classification: C07H,C12N,C12P,C12Y

Abstract:
A method to produce glucosamine by fermentation, comprising:a. culturing in a fermentation medium comprising assimilable sources of carbon, nitrogen and phosphate, a microorganism which comprises at least one genetic modification that increases the activity of glucosamine-6-phosphate synthase, wherein said genetic modification is selected from the group consisting of: i. transformation of said microorganisn with a recombinant nucleic acid molecule encoding glucosamine-6-phosphate synthase from E. coli which has glucosamine-6-phosphate synthase activity; and ii. genetic modification of a gene encoding glucosamine-6-phosphate synthase from E. coli that increases the activity of said glucosamine-6-phosphate synthase, wherein said genetic modification results in at least one nucleic acid modification selected from the group consisting of deletion, insertion, and substitution of at least one nucleotide of said gene encoding glucosamine-6-phosphate synthase, said at least one nucleotide modification resulting in increased glucosamine-6-phosphate synthase activity; wherein said step of culturing produces and accumulates a product selected from the group consistng of glucosamine-6-phosphate and glucosamine from said microorganism; and b. recovering and purifying said product.