Patent ID: 6264920
Filing Date: 2001-07-24
Classification: A61K,A61P

Abstract:
A method for restoring in vivo or in vitro fluorescence by adding one to fifty percent biocompatible organic solvents to the diagnostic or therapeutic compositions of dye molecules wherein said dye molecules are represented by the formula: ##STR7##wherein a.sub.4 and b.sub.4 are independently from 0 to 5; W.sup.4 and X.sup.4 are independently selected from the group consisting of (C((CH.sub.2).sub.zz OH).sub.2, C((CH.sub.2).sub.zz CO.sub.2 H).sub.2, C((CH.sub.2).sub.zz NR.sub.pp R.sub.pz).sub.2 ; and zz is from 1 to 5; R.sub.pp and R.sub.pz are selected from the group consisting of --(CH.sub.2).sub.c --CO.sub.2 H, --CH.sub.2 --(CH.sub.2 --O--CH.sub.2).sub.d --CH.sub.2 CO.sub.2 H, --(CH.sub.2).sub.g --N(R.sup.14)--(CH.sub.2).sub.h --CO.sub.2 H, and --(CH.sub.2).sub.i --N(R.sup.15)--CH.sub.2 --(CH.sub.2 --O--CH.sub.2).sub.j --CH.sub.2 --CO.sub.2 H; Y.sup.4 and Z.sup.4 are independently selected from the group consisting of --(CH.sub.2).sub.c --CO.sub.2 H, --CH.sub.2 --(CH.sub.2 --O--CH.sub.2).sub.d --CH.sub.2 CO.sub.2 H, --(CH.sub.2).sub.e --NH.sub.2, --CH.sub.2 --(CH.sub.2 --O--CH.sub.2).sub.f --CH.sub.2 --NH.sub.2, --(CH.sub.2).sub.g --N(R.sup.14)--(CH.sub.2).sub.h --CO.sub.2 H, and --(CH.sub.2).sub.i --N(R.sup.15)--CH.sub.2 --(CH.sub.2 --O--CH.sub.2).sub.j --CH.sub.2 --CO.sub.2 H; A.sub.2 is a single or a double bond; if A.sub.2 is a single bond, then B.sub.2 is independently selected from the group consisting of --O--, --S--, --Se--, --P-- and --NR.sup.38 and D.sub.2 is CR.sup.42 ; if A.sub.2 is a double bond, then B.sub.2 is selected from the group consisting of --O--, --S--, --Se--, --P-- and --NR.sup.38, C.sub.2 is selected from nitrogen and --CR.sup.41 and D.sub.2 is --CR.sup.42 ; R.sup.45 to R.sup.57 are independently selected from the group consisting of hydrogen, C1-C10 alkyl C1-C10 alkoxyl, cyano, nitro, halogen, and --NR.sup.43 R.sup.44 ; R R.sup.14, R.sup.15 and R.sup.38 to R.sup.42 are selected from the group consisting of -hydrogen, C1-C10 alkyl, C1-C10 alkoxyl --CH.sub.2 (CH.sub.2 --O--CH.sub.2).sub.c --CH.sub.2 --OH, --(CH.sub.2).sub.d --CO.sub.2 H, --CH.sub.2 --(CH.sub.2 --O--CH.sub.2).sub.e --CH.sub.2 --CO.sub.2 H, --(CH.sub.2).sub.f --NH.sub.2, and --CH.sub.2 --(CH.sub.2 --O--CH.sub.2).sub.g --CH.sub.2 --NH.sub.2 ; c, e, g, h, and i are independently from 1 to 10; d, f and j are independently from 1 to 100; and R.sup.43 and R.sup.44 are selected from the group consisting of hydrogen and C1-C10 alkyl.