Patent ID: 6984488
Filing Date: 2006-01-10
Classification: A61K,C07K,C12N,C12Q,C40B,G01N,Y02A

Abstract:
1. A method of identifying and producing a peptide that interacts with a ligand that interacts with a discontinuous epitope of a single biological unit consisting of a single protein, or consisting of two or more proteins that interact to form a complex, the method comprising: (a) providing a plurality of DNA fragments consisting of fragments, each of which appears in a DNA sequence that encodes the single biological unit; (b) creating a library consisting of oligonucleotides from said plurality of DNA fragments, each said oligonucleotide comprising at least two contiguous fragments, said fragments being randomly ligated such that any oligonucleotide in the library can ligate with any other oligonucleotide in the library; (c) inserting each of said oligonucleotides from said library of oligonucleotides into an expression system; (d) causing the peptides encoded by said oligonucleotides to be expressed; (e) screening the expressed peptides for interaction with a ligand that interacts with a discontinuous epitope of said single biological unit; (f) identifying any peptide which so interacts; and (g) producing any peptide so identified.