Patent ID: 8598337
Filing Date: 2013-12-03
Classification: A61K,C08B

Abstract:
1. A method of purifying a polysaccharide from a stock, wherein the stock comprises the polysaccharide and cellular components, the method comprising: contacting the stock with a first reagent to form a first mixture, wherein the first reagent is a base and wherein the pH of the first mixture is in the range of 9-15; separating the polysaccharide from at least some of the cellular components to form a separated composition comprising the polysaccharide and a residual amount of the cellular components; contacting the separated composition with a second reagent to form a second mixture, the second reagent being a base and wherein the pH of the second mixture is in the range of 9-15 and contacting is carried out at 40° C.-100° C.; and purifying the polysaccharide from at least some of the residual amount of the cellular components to form a purified composition:, wherein the polysaccharide is derived from wherein the second contacting step and the purifying step do not comprise column chromatography.