Patent ID: 7618775
Filing Date: 2009-11-17
Classification: A61K,A61P,C12N,C12Y,Y10S

Abstract:
1. A composition comprising a recombinant mutant GAL4 protein, or portion thereof, in a eukaryotic cell, wherein the mutant GALA protein, or portion thereof, comprises at least one unnatural amino acid selected from the group consisting of a p-acetyl-L-phenylalanine (1), p-benzoyl-L-phenylalanine (2), p-azido-L-phenylalanine (3), O-methy-L-tyrosine (4), and p-iodo-L-phenylalanine (5) and wherein the GAL4 protein, or portion thereof is at least 90% identical to a GAL4 protein encoded by a polynucleotide amplified from vector pCL1using a forward PCR primer comprising a nucleotide sequence set forth in SEQ ID NO: 103 and a reverse PCR primer comprising a nucleotide sequence set forth in SEQ ID NO: 104 that comprises a full-length wild-type N-terminal DNA binding domain and a full-length wild-type C-terminal activation domain, and wherein the recombinant mutant GALA protein is capable of activating a GAL4 responsive gene.