Patent ID: 8592157
Filing Date: 2013-11-26
Classification: B01D,B01L,C12M,C12N,G01N,Y10T

Abstract:
1. A method for extracting nucleic acid from a sample, the sample containing cells, spores, or microorganisms, the method comprising the steps of: a) introducing the sample into a cartridge having: b) contacting the sample with paper or membrane material in the lysing chamber, the paper or membrane material being impregnated with at least one chemical for lysing the cells, spores, or microorganisms in the sample, wherein the sample is dried on the paper or membrane material by heating the sample and absorbing moisture with the desiccant; c) lysing the cells, spores, or microorganisms with the chemical to release the nucleic acid from the cells, spores, or microorganisms; d) removing the nucleic acid from the lysing chamber by placing fluid in the lysing chamber, releasing the nucleic acid from the paper or membrane material into the fluid, and forcing the fluid containing the nucleic acid to flow out of the lysing chamber and through the capture region, thereby capturing the nucleic acid from the fluid with the capture material in the capture region; e) forcing the fluid that has flowed through the capture region to flow into the waste chamber; and f) eluting the captured nucleic acid from the capture region and forcing the eluted nucleic acid to flow into a reaction vessel coupled to the cartridge.