Patent ID: 7501494
Filing Date: 2009-03-10
Classification: A61K,A61P,C07K

Abstract:
1. A deimmunized recombinant monoclonal antibody expressed from the fusion of nucleic acids encoding a deimmunized Fv fragment of a murine antibody B4 comprising V H and Vκ variable chains and complementarity determining regions of the variable chains of said murine antibody B4 SEQ ID NOS: 20, 22, 24, 26, 28 and 30 and nucleic acids encoding human immunoglobulin constant regions, wherein said deimmunized Fv fragment of murine antibody B4 is deimmunized by replacing the murine heavy V H chain from the Fv domains of said murine antibody B4 with a nucleotide sequence encoding a deimmunized V H chain of murine antibody B4 selected from the group consisting of DIVHv.1 of SEQ ID NO: 5, DIVHv.2 of SEQ ID NO: 6, DIVHv.3 of SEQ ID NO: 7, and DIVHv.4 of SEQ ID NO: 8 and replacing the Vκ chain from the Fv domains of said murine antibody B4 with a nucleotide sequence encoding a deimmunized Vκ chain of murine antibody B4 selected from the group consisting of DIVKv.1 of SEQ ID NO: 9, DIVKv.2 of SEQ ID NO: 10 and DIVKv.3 of SEQ ID NO: 11.