Patent ID: 8247180
Filing Date: 2012-08-21
Classification: G01N,Y10S

Abstract:
1. A method of detecting a homodimer comprising a dimer of a membrane-associated analyte on a cell membrane, the method comprising the steps of: (a) providing a first binding compound specific for a first antigenic determinant of the membrane-associated analyte, wherein the first binding compound has a first molecular tag attached thereto by a cleavable linkage, and wherein the first molecular tag has a first separation characteristic; (b) providing a second binding compound specific for a second antigenic determinant of the membrane-associated analyte, wherein the second binding compound has a second molecular tag attached thereto by a cleavable linkage, and wherein the second molecular tag has a second separation characteristic that is different than the first separation characteristic of the first molecular tag of the first binding compound; (c) providing a cleaving probe specific for the first antigenic determinant of the membrane-associated analyte, wherein: (d) mixing the cleaving probe, the first binding compound, the second binding compound, and the cell membrane such that (e) separating and detecting the released molecular tags to determine the presence or the amount of homodimer in the cell membrane.