Patent ID: 6383769
Filing Date: 2002-05-07
Classification: C12N,C12Y,G01N

Abstract:
A method for fructofuranosyl transfer comprising reacting a fructofuranosyl donor with a fructofuranosyl acceptor in the presence of a polypeptide to transfer a fructofuranosyl residue from said fructofuranosyl donor to said fructofuranosyl acceptor, said polypeptide having &bgr;-fructofuranosidase activity, said polypeptide being obtainable by using a microorganism as a host and by expressing in said host a gene derived from a microorganism of the genus Bacillus, containing the amino acid sequences of SEQ ID NOs:1 and 2 as partial amino acid sequences, and having the following physicochemical properties:(1) Molecular weight about 44,000 to 54,000 daltons on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE); (2) Optimum pH about 5.5-6.0 when incubated at 10Â° C. for 10 min; (3) Optimum temperature about 45Â° C. and about 50Â° C. in the absence of and in the presence of calcium ion, respectively, when incubated at pH 6.0 for 10 min; (4) pH stability stable at a pH of about 5.0-8.0 when incubated at 4Â° C. for 24 hours; and (5) thermal stability stable up to a temperature of about 45Â° C. when incubated at pH 6.0 for an hour.