Patent ID: 6096551
Filing Date: 2000-08-01
Classification: A61K,C07H,C07K,C12N,C40B

Abstract:
An oligonucleotide primer for inducing mutagenesis in a complementarity determining region (CDR) of an immunoglobulin light chain gene, said oligonucleotide having 3' and 5' termini and comprising:a) a nucleotide sequence at said 3' terminus that hybridizes to a first framework region of an immunoglobulin gene;b) a nucleotide sequence at said 5' terminus that hybridizes to a second framework region of an immunoglobulin gene; andc) a nucleotide sequence between said 3' and 5' termini according to the formula:[NNK].sub.n,wherein N is independently any nucleotide, K is G or T, n is 3 to about 24, said 3' and 5' terminal nucleotide sequences having a length of about 6 to 50 nucleotides, or an oligonucleotide having a sequence complementary thereto, wherein said 5' terminus has the nucleotide sequence 5'-TATACTGTCAGCAGTAT-3' (SEQ ID NO 26) or 5'-GATTTTGCAGTGTATTACTGTCAGCAGTAT-3' (SEQ ID NO 27), or an oligonucleotide having a sequence complementary thereto.