Patent ID: 6013516
Filing Date: 2000-01-11
Classification: A61K,C07K,C12N

Abstract:
A method of producing a lentivirus which infects non-dividing cells, said method comprising:a) transfecting a suitable packaging host cell with the following vectors:a first vector providing a nucleic acid encoding a lentiviral gag and a lentiviral pol wherein the gag and pol nucleic acid sequences are operably linked to a heterologous regulatory nucleic acid sequence and wherein the vector is defective for nucleic acid sequence encoding functional ENV protein and wherein the nucleic acid of the first vector is devoid of lentiviral sequences both upstream and downstream from a splice donor site to a gag initiation site of a lentiviral genome;a second vector providing a nucleic acid encoding a non-lentiviral ENV protein;a third vector providing a nucleic acid sequence containing a lentiviral packaging signal flanked by lentiviral cis-acting nucleic acid sequences for reverse transcription, packaging and integration; a heterologous nucleic acid sequence, operably linked to a regulatory nucleic acid sequence; and a less than full length gag structural gene; andb) recovering the recombinant virus.