Patent ID: 7655783
Filing Date: 2010-02-02
Classification: C07K

Abstract:
1. An isolated, recombinant or synthetic polynucleotide encoding a variant antibody heavy chain lacking at least one amino acid in an antibody hinge region capable of forming a disulfide linkage, made by a method comprising: (a) providing a nucleic acid encoding an antibody heavy chain comprising an amino acid residue in an antibody hinge region capable of forming at least one disulfide linkage; (b) modifying the nucleic acid to encode a variant of the antibody lacking at least one amino acid residue in the antibody hinge region capable of forming at least one disulfide linkage; and (c) adding to the modified nucleic acid of (b), a nucleic acid encoding a prokaryotic secretion signal sequence or a periplasmic secretion sequence.