Patent ID: 9120891
Filing Date: 2015-09-01
Classification: C08G,C12P

Abstract:
1. A method of preparing polylactate (PLA), comprising: preparing a mutant microorganism in which a gene coding for an enzyme participating in an acetate-producing pathway is inactivated in the mutant microorganism or a gene coding for an enzyme re-converting acetate into acetyl CoA is amplified by over-expression of a gene to increase the productivity of the CoA donor, and a gene coding for an enzyme participating in conversion of pyruvate into lactate is amplified by over-expression of a gene to increase the productivity of the lactate; incubating the mutant microorganism in a medium containing glucose or glucose and hydroxyalkanoate; and recovering PLA from the mutant microorganism, wherein the enzyme participating in the acetate-producing pathway is acetate kinase A, wherein the enzyme re-converting the acetate into the acetyl CoA is acetyl-CoA synthetase, and wherein the enzyme participating in the conversion of the pyruvate into the lactate is lactate dehydrogenase A.