Patent ID: 9207239
Filing Date: 2015-12-08
Classification: B01L,G01N

Abstract:
1. A test cartridge for assaying for possible infection or sepsis in a subject, comprising a single use microfluidic cartridge that is adapted to receive a blood sample from a subject, the microfluidic cartridge comprising: a) a sample composition chamber adapted for receiving a blood sample from a subject; b) a first pre-filled microfluidic blister comprising an antibody mixture comprising fluorescently tagged CD64 and fluorescently tagged CD163 antibodies; c) a second pre-filled microfluidic blister comprising a cell lysis reagent; d) a third pre-filled microfluidic blister comprising fluorescently tagged beads comprising two fluorescent tags, wherein at least one of the two fluorescent tags is different than the fluorescently tagged CD64 and fluorescently tagged CD163 antibodies; d) a treatment compartment adapted for fluid mixing, wherein the treatment compartment is in fluid communication with the sample composition chamber, the first pre-filled microfluidic blister, the second pre-filled microfluidic blister, and the third pre-filled microfluidic blister; e) a pump connected to the treatment compartment; and f) an evaluation chamber fluidly connected to the treatment chamber and comprising a reading zone.