Patent ID: 6391315
Filing Date: 2002-05-21
Classification: A61K,A61P,C07K

Abstract:
An antigenic composition prepared by culturing a broth of a pathogenic bacterium selected from the group consisting of Staphylococcus aureus and Staphylococcus epidermidis, centrifuging the cultured broth, separating a supernatant from whole cells within the cultured broth, precipitating components separately from the supernatant and said whole cells and removing the precipitated components from residual components of the supernatant and whole cells, preparing a mixed solution that is substantially free of said residual components by combining precipitates from said whole cells and said supernatant, and subjecting said mixed solution to column chromatography to isolate said antigenic composition as a rabbit red blood cell hemolysis positive fraction consisting of components having molecular weight of about 10,000-70,000, said composition comprising as main constituents TSST-1, hemolysin, and enterotoxin, said antigenic composition further being free of endotoxin and whole cells.