Patent ID: 6723525
Filing Date: 2004-04-20
Classification: A61P,C12Q,G01N

Abstract:
A method of selecting or identifying a compound that modulates, inhibits or activates the activity of a lipid kinase, comprising (i) mixing the lipid kinase and a labeled lipid substrate thereof in the presence of a candidate compound and a source of phosphate to form a reaction mixture, (ii) exposing the reaction mixture of (i) to a support material, wherein the support material binds lipid substrate in a phosphorylated form and essentially does not bind an unphosphorylated lipid, and (iii) assessing the amount of phosphorylated lipid bound to the support, said amount being an indication of the activity of said lipid kinase, allowing the selection or identification of a compound that modulates, inhibits or activates said activity.