Patent ID: 8170804
Filing Date: 2012-05-01
Classification: C12Q,G06F

Abstract:
1. A computer-implemented method of determining a melting temperature, Tm, of DNA, the method comprising the steps, implemented in a computer system having a processor, of: receiving a dataset representing, a melt curve for a DNA sample, the dataset including a plurality of data points each having a pair of coordinate values; numerically determining first derivative values for data points of the melt curve; determining a baseline for the first derivative values; subtracting out the baseline from the first derivative values to produce modified first derivative values; determining a first maximum value of the modified first derivative values; calculating an approximation of a curve that fits the modified first derivative values by applying a regression process to a Gaussian Mixture Model function to determine one or more parameters of the function, wherein said parameters include initial conditions, and wherein the first maximum value is used as an initial condition for a first parameter; and outputting the first parameter, wherein the first parameter represents a melting temperature Tm, of the DNA sample.