Patent ID: 8734871
Filing Date: 2014-05-27
Classification: A61K,A61P

Abstract:
1. An isolated glycolipid fraction from Oscillatoria Planktothrix sp. for treating and/or reducing the risk of developing one or more gingival pathologies have a bacterial aetiology, wherein the isolated glycolipid fraction is obtained by a) suspending a pellet of the b) mixing the cyanobacterial suspension with a denaturing solution comprising: a caotropic agent, a erotic organic solvent and an aprotic organic solvent, in a volume ratio comprised from 1:2 to 1:4, c) incubating for a time lower than 60 minutes; d) centrifugating and collecting the supernatant; e) precipitating the glycolipid fraction by addition of: a salt and an organic solvent; washing the pellet with water-diluted ethanol; f) re-suspending the pellet in an aqueous solution; g) treating the solution with nucleases; then treating the solution with a protease; h) precipitating further the glycolipid fraction, by addition of: a salt and an organic solvent; i) optional washing the pellet as in step f); further re-suspending it in an aqueous, solution; j) molecularly separating the re-suspended solution with a 30 k Dalton cut-off device; k) recovering the glycolipid fraction retained by the molecular separation of step j) with an aqueous, buffered solution and evaluating the protein and nucleic acids contamination degree, and wherein said glycolipid fraction has a degree of protein contamination below or equal to 2% and a degree of nucleic acids contamination below or equal to 5% by weight within the total glycolipid fraction.