Patent ID: 7510719
Filing Date: 2009-03-31
Classification: A61K,A61P,C12N

Abstract:
1. A method of increasing the yield of attenuated cold adapted reassortant influenza B virus particles, the method comprising: a. introducing a plurality of attenuated cold adapted reassortant influenza B viruses into SPF embryonated hen eggs; b. incubating the host eggs at a temperature of 31±0.5° C.; and, c. recovering influenza virus particles from the host eggs, wherein the recovered virus particles resulting from the incubation has a yield which is greater than the yield resulting from an incubation of said attenuated cold adapted reassortant influenza B viruses in host eggs at 33° C., and wherein said attenuated cold adapted reassortant influenza B viruses comprise a partial genome of the B/Ann Arbor/1/66 master donor virus.