Patent ID: 7393659
Filing Date: 2008-07-01
Classification: C12Q,G01N

Abstract:
1. A method for determining the digestibility of proteins in a nutritional product comprising the steps of: a) providing a sample of a nutritional product that contains protein and a reagent blank; b) separately digesting the sample of a nutritional product and the reagent blank with at least one protein-degrading enzyme; c) terminating each said digestion process to obtain a digested sample and a digested reagent blank; d) removing a first portion from each of said digested sample and said digested reagent blank in step c); e) determining the concentrations of at least 18 different amino acids, including tryptophan, for each of said first portions obtained from said digested sample and said digested blank in step d); f) removing a second portion from each of said digested sample and said digested reagent blank in step c); g) separating each of said second portions from step f) into a liquid phase and a solid phase; h) determining the concentrations of said at least 18 different amino acids, including tryptophan, soluble in the liquid phases obtained from said second portions of said digested sample and said digested reagent blank in step g); i) calculating the percent digestibility of the protein in the nutritional sample by: thereby determining the protein digestibility of a nutritional sample.