Patent ID: 8637287
Filing Date: 2014-01-28
Classification: C12N,C12P,C12Q,G01N

Abstract:
1. An isolated mutant glycosyltransferase comprising: (a) the amino acid sequence of OleD glycosyltransferase set forth in SEQ ID NO: 1, wherein proline at position 67 has been replaced with threonine, serine at position 132 has been replaced with phenylalanine, alanine at position 242 has been replaced with leucine, and glutamine at position 268 has been replaced with valine; or (b) an amino acid sequence which has at least 95% sequence identity to SEQ ID NO:1, wherein the proline at position 67 has been replaced with threonine, the serine at position 132 has been replaced with phenylalanine, the alanine at position 242 has been replaced with leucine, and the glutamine at position 268 has been replaced with valine, wherein said isolated mutant exhibits an improved conversion of nucleotide diphosphate (NDP) to NDP sugar as compared to a corresponding non-mutated glycosyltransferase.