Patent ID: 7927798
Filing Date: 2011-04-19
Classification: C12Q

Abstract:
1. A method of detecting at least a first target nucleic acid, the method comprising: providing a sample comprising whole blood, which whole blood comprises peripheral blood cells; lysing the peripheral blood cells in the whole blood to produce a whole blood lysate, which whole blood lysate comprises the first target nucleic acid; contacting the peripheral blood cells and/or the whole blood lysate with an exogenously supplied protease; contacting the first target nucleic acid with a first set of n capture extenders, wherein n is at least two, which first set of capture extenders is capable of hybridizing to the first target nucleic acid, wherein contacting the first target nucleic acid with the first set of capture extenders comprises contacting the whole blood lysate with the first set of capture extenders; hybridizing the first target nucleic acid to the first set of capture extenders; providing a solid support, wherein a first capture probe is bound to the solid support; associating the first set of capture extenders with the solid support by hybridizing the capture extenders to the first capture probe at a hybridization temperature which is greater than a melting temperature (T whereby hybridizing the first target nucleic acid to the first set of capture extenders and associating the first set of capture extenders with the solid support captures the first target nucleic acid on the solid support; and detecting, while the first target nucleic acid is captured on the solid support, the presence of the first target nucleic acid on the solid support.