Patent ID: 6040174
Filing Date: 2000-03-21
Classification: A61K,C07K,C12N

Abstract:
A complementation line comprising a complementation element for complementing in trans a defective adenoviral vector,wherein said complementation element is either integrated into the chromosomal genome of said complementation line or inserted into an expression vector and said complementation element comprises a fragment of an adenoviral genome lacking an adenoviral 5'ITR and encoding an E1A gene product,wherein the E1A gene product is expressed under control of:a) an E1 promoter or a heterologous promoter when the complementation line is made from a human embryonic retinal cell or A549 cell;b) an E1 promoter or a heterologous promoter when the complementation line is made by cotransfection of the expression vector comprising the complementation element and the defective adenoviral vector; orc) an inducible heterologous promoter.