Patent ID: 6132957
Filing Date: 2000-10-17
Classification: C07H,C12Q

Abstract:
A method for the determination of inhibition of adenylyl cyclase activity, comprising the steps of:a) contacting a 9-substituted adenine with a cell extract containing adenylyl cyclase and expressing adenylyl cyclase activity;b) detecting the amount of inhibition of adenylyl cyclase activity, andc) correlating the result from step b) with a previously established test standard or control wherein a decrease in the amount of adenylyl cyclase activity over the control is indicative of inhibition;wherein the 9-substituted adenine is selected from the group consisting of;(19) 9-(cyclopentyl)-2-fluoro-9H-adenine;(20) 9-(4-benzoylbenzyl)-9H-adenine;(21) 9-[(4-bisphenyl)butenyl-4-yl]-9H-adenine;(22) 9-(3-monophosphoryl-cyclopentyl)-adenine;(23) 9-(3-diphosphoryl-cyclopentyl)-adenine;(24) 9-(3-triphosphoryl-cyclopentyl)-adenine;(25) 9-[3-(2-O-thiodiphosphoryl)-cyclopentyl]-adenine; and(26) 9-[3-(3-O-thiodiphosphoryl)-cyclopentyl]-adenine.