Patent ID: 6251604
Filing Date: 2001-06-26
Classification: C12N

Abstract:
A method for producing a library of mutagenized polynucleotides from a target sequence comprising:forming a sample comprising(i) a target sequence including a section to be mutagenized,(ii) a first primer including a sequence complementary to a 3' sequence of a sense strand of the section of the target sequence,(iii) a second primer including a sequence complementary to a 3' sequence of an antisense strand of the section of the target sequence, and(iv) a library of oligonucleotides; andperforming multiple cycles of primer extension amplification on the sample using a polymerase where primer extension is performed under conditions suitable for the first and second primers to be extended and for the oligonucleotides to anneal to a portion of the target sequence or amplification products thereof which the oligonucleotides are not known to anneal to form an imperfect double-stranded sequence and be extended by the polymerase;wherein a library of mutagenized polynucleotides are produced as amplification products of the multiple amplification cycles.