Patent ID: 7939260
Filing Date: 2011-05-10
Classification: C12P,C12Q

Abstract:
1. A method for making available a priming oligonucleotide for extension in the presence of a target nucleic acid, the method comprising the steps of: (a) forming a reaction mixture comprising the priming oligonucleotide and the target nucleic acid, the priming oligonucleotide having a cap hybridized to a region at the 3′-end thereof, wherein the 5′-terminal base of the cap is complementary to the 3′-terminal base of the priming oligonucleotide, and wherein the cap is modified to prevent the initiation of DNA synthesis therefrom; (b) hybridizing the priming oligonucleotide to a target sequence contained within the target nucleic acid, thereby displacing the cap from the priming oligonucleotide; and (c) extending the priming oligonucleotide in a primer extension reaction with a DNA polymerase to give a DNA primer extension product complementary to the target sequence.