Patent ID: 6268154
Filing Date: 2001-07-31
Classification: C07K,C12N,C12R

Abstract:
A method for detecting and determining the quantity of bacteria that oxidize nitrite to nitrate in a media, wherein the 16S rDNA of the bacteria has a nucleotide sequence of SEQ ID NO:1, said method comprising the steps of:(a) providing a detectably labeled probe selected from the group consisting of SEQ ID NO: 2 and SEQ ID NO: 3;(b) isolating total DNA from the media;(c) exposing the isolated total DNA to the detectably labeled probe under conditions under which the probe hybridizes to only the nucleic acid of bacteria having 165 r DNA that has a nucleotide sequence of SEQ ID.NO. 1; and(d) detecting and measuring the amount of hybridized probe, wherein the presence of hybridized probe is indicative of the presence of bacteria that oxidize nitrite to nitrate and the amount of hybridized probe is indicative of the quantity of said bacteria that oxidize nitrite to nitrate in said media.