Patent ID: 6793926
Filing Date: 2004-09-21
Classification: C12N

Abstract:
A method for producing a recombinant adeno-associated virus (AAV), comprising the steps of:a) infecting a mammalian host cell with a carrier virus, wherein said host cell comprises nucleic acid encoding proteins required for replication and encapsidation of the AAV stably integrated in its genome, wherein the carrier virus comprises i. a non-mammalian virus nucleic acid backbone that comprises a recombinant AAV genome comprising AAV ITRs and a transgene, ii. nucleic acid encoding adenovirus helper function; and iii. optionally comprises nucleic acid encoding a ligand expressed on the surface of the non-mammalian virus wherein the ligand binds a mammalian host cell; and b) growing the infected mammalian host cell under conditions in which the recombinant AAV genome is replicated, excised and encapsidated.