Patent ID: 9068174
Filing Date: 2015-06-30
Classification: A61K,A61P,C12N,C12Y

Abstract:
1. A method for sensitizing tumor cells to CPT-11 comprising directly injecting a tumor site comprising tumor cells with a composition comprising an isolated polynucleotide comprising a tumor-specific responsive promoter operably linked to a nucleic acid molecule encoding a mutant human carboxylesterase consisting of the amino acid sequence as set forth in SEQ ID NO: 3, wherein the tumor-specific responsive promoter is selected from the group consisting of a carcinoembryonic antigen promoter, a HER-2/neu promoter, a tumor-specific responsive promoter comprising Myc-Max response elements, and a DF3/MUC promoter, so that said tumor cells express the mutant human carboxylesterase and are sensitized to CPT-11.