Patent ID: 8951938
Filing Date: 2015-02-10
Classification: G01N,Y10S

Abstract:
1. A method of distinguishing, in a sample, distinct states resulting from cellular differentiation in forms of a cell type of interest and one or more differentiated forms of the cell type of interest, said method comprising: providing a sample containing a cell type of interest and one or more differentiated forms of the cell type of interest; providing a rosamine derivative compound of the formula: wherein: X is O, NR R R wherein the substituted form of R 2 has one or more substituents independently selected from the group consisting of halogen, NR 4 R 5 , OR 7 , SR 4 , aryl, C 1 to C 6 alkyl, R R R R R R Y is alkyl or halogen; n is 1 to 3; m is 0 to 3; and * is a site on a substituent which binds to the rosamine derivative compound, wherein the rosamine compound produces differing fluorescent signals for the cell type of interest than for the one or more differentiated forms of the cell type of interest; contacting the sample with the rosamine derivative compound under conditions effective to produce differing fluorescent signals for the cell type of interest than for the one or more differentiated forms of the cell type of interest; and detecting and distinguishing, as distinct states resulting from cellular differentiation, the presence of the cell type of interest and the presence of one or more differentiated forms of the cell type of interest based on fluorescent signals emitted by the sample following said contacting.