Patent ID: 6238884
Filing Date: 2001-05-29
Classification: C12N,C12Q

Abstract:
A method for producing a mutant polynucleotide encoding at least one desirable property, the method comprising:(a) subjecting a plurality of first polynucleotides to simultaneous mutagenesis so as to produce a plurality of progeny polynucleotides, wherein the mutagenesis comprises subjecting a codon-containing template polynucleotide to amplification using a degenerate oligonucleotide for each codon to be mutagenized, wherein the degenerate oligonucleotide comprises a first homologous sequence and a degenerate triplet sequence, and(b) subjecting the progeny polynucleotides to an end selection-based screening and enrichment process that creates ligation-compatible ends, so as to select one or more progeny polynucleotides encoding at least one desirable property.