Patent ID: 8086410
Filing Date: 2011-12-27
Classification: G16B

Abstract:
1. A method for detecting insertion and deletion in a sample DNA sequence, comprising: using a computer to align trace data of a sample DNA sequence that is aligned to trace data of a reference DNA sequence to produce an aligned sample DNA sequence, whereby the trace data of the reference DNA sequence and the trace data of the sample DNA sequence include frame numbers; using a computer to chose pairs of data points from any of the trace data for different positions along the trace data of the reference DNA sequence or the sample DNA sequence before alignment, whereby the number of frame numbers between the chosen pairs is the same for each chosen pair; using a computer to locate the chosen pairs of data points chosen from either the reference DNA sequence or the sample DNA sequence in the aligned sample DNA sequence; using a computer to measure the number of frame numbers between the located chosen pairs of data points in the aligned sample DNA sequence; and finding any increase and decrease of number of frame numbers from the measured number of frame numbers of the aligned sample sequence as compared to the same number of frame numbers used when choosing the paired data points from either the reference DNA sequence or the sample DNA sequence before alignment, whereby a dramatic increase is an insertion and a dramatic decrease is a deletion if the chosen points are from the reference DNA sequence and whereby a dramatic increase is a deletion and a dramatic decrease is an insertion if the chosen points are from the sample DNA sequence before alignment.