Patent ID: 9057729
Filing Date: 2015-06-16
Classification: C12Q,G01N,G06K,G06T

Abstract:
1. A method for detecting a ploidy state of a cell of interest in a biological tissue sample without fluorescent markers that interact with double stranded nucleic acids, the method comprising: scanning through a single plane of the biological tissue sample, wherein the first scan traverses the cell of interest, and wherein the first scan traverses the cell's nucleus and non-nuclear portions of the cell of interest; measuring an ultraviolet (UV) light absorbance within the nuclear and non-nuclear portions of the cell of interest during the first scan; scanning through at least a second plane of the biological tissue sample, wherein the second scan traverses the cell of interest, and wherein the second scan traverses nuclear and non-nuclear portions of the cell of interest; measuring the UV light absorbance within the nuclear and non-nuclear portions of the cell of interest during the second scan; reconstructing a three-dimensional (3D) volume of the nucleus of the cell of interest from the combined scans, wherein a ratio between the measurement for the UV nuclear absorption versus the UV non-nuclear absorption from each plane is taken as a calibrated measurement for the UV absorption of the cell nucleus; and determining the ploidy state of the cell of interest from the UV absorbance of the whole nucleus and the reconstructed 3D volume, wherein a measure for UV absorption of the whole nucleus is obtained by summing up the calibrated measurements of the scans.