Patent ID: 8106179
Filing Date: 2012-01-31
Classification: A01K,A61K,A61P,C12N,C12Y

Abstract:
1. A double-stranded oligonucleotide comprising two strands of 19 to 23 nucleotides, each strand consisting, from 5′ to 3′, of a sequence of 17 to 21 ribonucleotides and two deoxyribo- or ribonucleotides, the 17 to 21 ribonucleotide RNA sequences of said strands being complementary and the two nucleotides of the 3′ ends being protruding, wherein the RNA sequence of the sense strand or positive strand is selected from the group consisting of a 17 to 21 ribonucleotide fragment of a transcript of a protein kinase CK2 beta-subunit which is included between positions 80-100, from the ATG codon, with reference to the human transcript sequence SEQ ID NO: 90, and a 17 to 21 ribonucleotide fragment having at least 80% identity with the preceding fragment, wherein the sequence of the sense strand or positive strand is selected from the group consisting of the sequences SEQ ID NO: 67, 83 and 86, and wherein the double-stranded oligonucleotide inhibits specifically more than 80% of the expression of the protein kinase CK2 beta-subunit and of the corresponding mRNA in human cell culture at a concentration of between 1 and 200 nM.