Patent ID: 8481974
Filing Date: 2013-07-09
Classification: G01J,G01N

Abstract:
1. An optical detection system, comprising: an optics control module, comprising, a microprocessor in electrical communication with a data acquisition (DAQ) recorder and with a charged coupled diode (CCD) camera, the DAQ recorder in electrical communication with first and second illumination source controls and an avalanche photodiodes (APD) shutter control in electrical communication with first and second dichroic fiber optic couplings, the DAQ recorder in further electrical communication with first and second APD optically communicating with the first and second dichroic fiber optic couplings; an optical detection module comprising an objective lens, a first long pass dichroic filter receiving light passed by the objective lens, a beam splitter, and the CCD camera, the beam splitter dividing the light passing through the first long pass dichroic filter between the CCD camera and a short pass dichroic filter passing only red wavelengths of light to the first dichroic fiber optic coupling, the short pass filter re-directing all remaining wavelengths of the light re-directed by the beam splitter into a second long pass filter configured to re-direct only yellow wavelengths of light into the second dichroic fiber optic coupling, wherein the first and second dichroic fiber optic couplings optically communicate with the first and second APD, respectively; a first LED light source comprising a source of actinic radiation having a wavelength of about 488 nm and a light source shutter in electrical communication with the first illumination source control, wherein control and operation of the light source shutter is performed by the microprocessor; a second LED light source comprising a source of actinic radiation having a wavelength of about 470 nm and a source of infrared radiation having a wavelength of greater than about 750 nm, the second light source in electrical communication with the second illumination source control wherein control and operation of the second LED light source is performed by the microprocessor; and a translatable x-y microscope stage, wherein the translatable microscope stage positions a sample of plant matter under the objective lens.