Patent ID: 8524497
Filing Date: 2013-09-03
Classification: A61K,A61P,C12N,Y02A

Abstract:
1. A method of cultivating mammalian surface-dependent cells to obtain a confluent cell culture, comprising: providing an animal protein-free medium comprising soy hydrolysate at a concentration of about 0.05% (w/v) to about 1% (w/v) and yeast hydrolysate at a concentration of about 0.05% (w/v) to about 0.3% (w/v); growing the surface-dependent cells in the medium; and passaging and sub-cultivating the cells grown in the medium in order to obtain a confluent cell culture; wherein the cells are infected with an orthomyxovirus and are selected from the grouping consisting of BSC-1 cells, CV-1 cells, COS cells, VERO cells, MDBK cells, MDCK cells, MDOK cells, BHK-21 cells, WI-38 cells, and MRC-5 cells.