Patent ID: 8604281
Filing Date: 2013-12-10
Classification: A61P,C12N

Abstract:
1. A process of producing transgenic multi-cellular plants or parts thereof expressing a trait (1) and a trait (2) of interest, wherein said process comprises (i) producing a first plant having in a first locus of a nuclear chromosome a first heterologous nucleotide sequence comprising: a first fragment of a nucleotide sequence conferring trait (1) and a first fragment of a nucleotide sequence conferring trait (2); (ii) producing a second plant having in a second locus of a nuclear chromosome homologous to said nuclear chromosome of step (i), a second heterologous nucleotide sequence comprising a second fragment of a nucleotide sequence conferring trait (1) and a second fragment of a nucleotide sequence conferring trait (2); (iii) hybridising said first and said second plant to generate progeny exhibiting said functional trait (1) due to intein-mediated trans-splicing between a protein or polypeptide encoded by said first fragment of a nucleotide sequence conferring trait (1) and a protein or polypeptide encoded by said second fragment of a nucleotide sequence conferring trait (1); and exhibiting said functional trait (2) due to intein-mediated trans-splicing between a protein or polypeptide encoded by said first fragment of a nucleotide sequence conferring trait (2) and a protein or polypeptide encoded by said second fragment of a nucleotide sequence conferring trait (2); and (iv) selecting progeny that exhibits said trait (2) by applying a herbicide to said progeny, whereby said trait (1) endows resistance against said herbicide.