Patent ID: 6664108
Filing Date: 2003-12-16
Classification: C07K,C12N

Abstract:
A method for providing to a plant cell a plurality of copies of a DNA sequence of interest flanked by nonidentical target sites comprising:a) providing an Agrobacterium replicon having a T-DNA, wherein said T-DNA comprises in a 5â€² to 3â€² or 3â€² to 5â€² orientation, a first target site for a first recombinase, a viral replicon a second target site for a second recombinase, said DNA sequence of interest, and a third target site for said first recombinase, wherein said first and said third target sites are directly repeated and identical with respect to each other, and said second target site is nonidentical to said first and said third target sites; and b) infecting the plant cell with an Agrobacterium carrying said Agrobacterium replicon under conditions that allow transfer of said T-DNA and expression of said first recombinase in said cell; wherein said plant cell, said T-DNA, or said viral replicon comprises a nucleotide sequence encoding at least said first recombinase or an active fragment or an active variant thereof wherein said active fragment or said active variant retains the ability to catalyze a recombination event, and said nucleotide sequence is operably linked to a promoter that drives expression in said plant cell.