Patent ID: 8956830
Filing Date: 2015-02-17
Classification: C12N,C12P

Abstract:
1. A method of producing a recombinant protein preparation having target values of high mannose glycans and fucosylated glycans, the method comprising: (a) providing a mammalian cell genetically engineered to express a recombinant protein; (b) culturing the cell in a culture medium comprising dimethylsulfoxide (DMSO) under conditions in which the cell expresses the recombinant protein; and (c) harvesting a preparation of the recombinant protein produced by the cell that meets the target values of the high mannose glycans and fucosylated glycans, wherein the target value of high mannose glycans is a level at least 20% higher than a level of high mannose glycans and the target value of fucosylated glycans is a level at least 10% lower than in a preparation produced by culturing the cell in the medium not comprising DMSO.