Patent ID: 8961995
Filing Date: 2015-02-24
Classification: A61K,C12N

Abstract:
1. A recombinant replicon nucleic acid comprising: a) a nucleic acid sequence encoding a first alphavirus 5′ untranslated region (5′ UTR) and a 51 nucleotide conserved sequence element (51 -nt CSE); b) a nucleic acid sequence encoding alphavirus nonstructural proteins nsP1, nsP2, nsP3 and nsP4; c) a nucleic acid sequence encoding an alphavirus subgenornic promoter; d) a nucleic acid sequence encoding a second alphavirus 5′ untranslated region (5′ UTR) and 51 nucleotide conserved sequence element (51 -nt CSE); e) a nucleic acid sequence encoding a protease; f) a nucleic acid sequence encoding a heterologous protein; and g) a nucleic acid sequence encoding an alphavirus 3′ untranslated region (3′ UTR).