Patent ID: 6326043
Filing Date: 2001-12-04
Classification: G01N

Abstract:
A process for preparing and using pure reference standards for estimating gelatinized starch content in a selected steam-flaked grain sample, comprising the steps of:(a) preparing a plurality of reference standards containing known proportions of intact starch and gelatinized starch;(b) enzymatically measuring the glucose yield as a percentage of total glucose in each said reference standard under set conditions;(c) establishing the relationship between the percentage of glucose yield to total glucose, and the percentage of gelatinized starch obtained from each reference standard, by plotting said percentage values to correspond on a graph prepared with separate axis designated as (1) the glucose yield as a percentage of total glucose, and (2) the percentage of gelatinized starch;(d) enzymatically measuring the yield of glucose as a percentage of total glucose in a steam-flaked grain sample under the same set conditions as used in step (b); and(e) plotting the value of the yield of glucose percentage for the steam-flaked grain sample from step (d) on the graph prepared in step (c), and comparing said steam-flaked grain value to the values of the reference standards found therein, to estimate the corresponding percentage of gelatinized starch in said steam-flaked grain sample.