Patent ID: 8921304
Filing Date: 2014-12-30
Classification: A61P,C07K,C12N,G01N

Abstract:
1. A non-naturally occurring protein that binds to the extradomain B (ED-B) of fibronectin, said protein comprising two monomeric ubiquitin units linked together in a head-to-tail arrangement, wherein: (i) each monomeric ubiquitin unit of said protein has at least 85% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 1; and amino acid substitutions selected from the group consisting of positions corresponding to residues 2, 4, 6, 8, 62, 63, 64, 65, and 66 of SEQ ID NO: 1, and further wherein: (ii) said protein has a specific binding affinity to said ED-B domain of fibronectin of K (iii) said protein exhibits a monovalent binding activity with respect to said ED-B domain of fibronectin.