Patent ID: 6197587
Filing Date: 2001-03-06
Classification: A01H,C12N

Abstract:
A method of inducing somatic embryogenesis in a cacao tissue comprising the steps of:(a) providing a cacao tissue explant;(b) culturing the explant on a primary callus growth medium having the property of inducing callus growth on the explant, wherein the primary callus growth medium comprises a DKW basal medium, a carbon source, and a growth regulator selected from a cytokinin, an auxin or a combination thereof;(c) culturing the callus produced in step (b) on a secondary callus growth medium, having the property of inducing homeostatic growth and bipolar callus cell development, wherein the secondary callus growth medium comprises a WPM low salt basal medium, a carbon source, and a growth regulator selected from a cytokinin, an auxin or a combination thereof; and(d) culturing the callus produced in step (c) on an embryo development medium having the property of inducing embryo differentiation, wherein the embryo development medium comprises a DKW basal medium and a carbon source.