Patent ID: 7816495
Filing Date: 2010-10-19
Classification: A61K,A61P,C07K

Abstract:
1. A method for the separation and purification of fibrinogen and plasminogen which comprises the steps of: (a) loading a solution comprising fibrinogen and plasminogen onto an immobilized metal ion affinity chromatography matrix under conditions such that the fibrinogen and the plasminogen both bind to the matrix, and (b) selectively eluting the fibrinogen and the plasminogen separately from the matrix, wherein the immobilized metal ion affinity chromatography matrix is a copper, nickel or zinc ion affinity chromatography matrix; wherein plasminogen is eluted using a buffer comprising less than or equal to 20 mM of a competitive chelating compound; wherein fibrinogen is eluted using a buffer comprising greater than 20 mM of the same or a different competitive chelating compound; and wherein the competitive chelating compound is selected from the group consisting of an amino acid, imidazole, EDTA and a citrate salt.