Patent ID: 6284517
Filing Date: 2001-09-04
Classification: C12Q,Y10S

Abstract:
Isolation plating medium for the simultaneous identification of Bacillus cereus and Bacillus thuringiensis consisting essentially of a mixture of (1) nutrients that promote growth of cells of Bacillus cereus and Bacillus thuringiensis under suitable environmental conditions for growth, (2) an ingredient that facilitates resuscitation of damaged Bacillus cereus and Bacillus thuringiensis cells under suitable environmental conditions for growth, (3) an ingredient that promotes germination of Bacillus spores under suitable environmental conditions for growth, (4) an ingredient that inhibits the growth of strains of related bacteria and Bacillus other than Bacillus cereus or Bacillus thuringiensis under suitable environmental conditions for growth, (5) an ingredient that inhibits the growth of yeast and molds, (6) a chromogenic substrate that changes color responsive to the presence of phosphatidylinositol-specific phospholipase C, and (7) an ingredient for thickening the mixture in sufficient quantity to solidify the mixture.