Patent ID: 6214559
Filing Date: 2001-04-10
Classification: A61K,C07K,Y10S

Abstract:
A method of identifying an inhibitor of IL-13 binding to the IL-13 receptor which comprises:(a) combining a receptor chain protein with IL-13 or a biologically active fragment thereof, said combination forming a first binding mixture;(b) measuring the amount of binding between the receptor chain protein and the IL-13 or fragment in the first binding mixture;(c) combining a compound with the receptor chain protein and the IL-13 or fragment to form a second binding mixture;(d) measuring the amount of binding in the second binding mixture; and(e) comparing the amount of binding in the first binding mixture with the amount of binding in the second binding mixture;wherein the compound is capable of inhibiting IL-13 binding to the IL-13 receptor when a decrease in the amount of binding of the second binding mixture occurs, and wherein said receptor chain protein comprises an amino acid sequence selected from the group consisting of:(1) the amino acid sequence of SEQ ID NO:2;(2) the amino acid sequence of SEQ ID NO:2 from amino acids 22 to 334;(3) the amino acid sequence of SEQ ID NO:2 from amino acids 357 to 383;(4) the amino acid sequence of SEQ ID NO:4;(5) the amino acid sequence of SEQ ID NO:4 from amino acids 26 to 341; and(6) the amino acid sequence of SEQ ID NO:4 from amino acids 363 to 380.