Patent ID: 8124106
Filing Date: 2012-02-28
Classification: C12N

Abstract:
1. A method for removing adenovirus proteins that are not incorporated into subgroup B recombinant human adenovirus particles from a preparation of subgroup B recombinant human adenovirus particles, the method comprising the steps of: subjecting a preparation of the recombinant adenovirus particles comprising adenovirus proteins that are not incorporated into adenovirus particles to a charged filter that contains anion exchange groups, wherein the preparation of the recombinant adenovirus particles comprises subgroup B recombinant human adenovirus particles thus removing adenovirus proteins that are not incorporated into the adenovirus particles, said adenovirus proteins comprising subgroup B adenoviral proteins II, III, IV, and 52.55 k; and analyzing the filtered preparation to determine whether subgroup B adenoviral 52.55 k protein that is not incorporated into adenovirus particles has been removed from the preparation.