Patent ID: 7625700
Filing Date: 2009-12-01
Classification: C07K,C12N,C12Q,C40B,G01N,Y02A

Abstract:
1. A method for identifying an interacting set of protein molecules comprising: A) generating fragments of a protein reporter molecule which have a directly or indirectly detectable activity when associated and wherein the association of said fragments is independent of other molecular processes; B) coupling first fragments of said protein reporter molecule to members of a first panel of protein molecules; C) coupling second fragments of said protein reporter molecule to members of a second panel of protein molecules; D) mixing the products of B) and C); E) directly or indirectly testing for said activity; and F) identifying the protein panel members whose interaction resulted in said activity and which thus form an interacting set with the proviso that said protein reporter molecule is not ubiquitin and said method is not a two hybrid method.