Patent ID: 7871789
Filing Date: 2011-01-18
Classification: C12Q,G01N

Abstract:
1. A method for assaying glycated albumin, comprising: adding a metalloprotease into a sample containing the glycated albumin so as to digest the glycated albumin with the metalloprotease; adding quaternary ammonium having a hydrocarbon group with a carbon number of 14 or more, or a salt of the quaternary ammonium before, at the same time of or after the adding of the metalloprotease, wherein a resultant concentration of the metalloprotease ranges from 1 KU/L to 1,600 KU/L, adding a fructosyl amino acid oxidase (FAOD) to the sample so that a glycated part of the digestion product of the glycated albumin reacts with the FAOD, wherein the FAOD is added before, at the same time or after the adding of the metalloprotease; measuring an amount of a redox reaction product generated by a redox reaction between the glycated part of the digestion product of the glycated albumin and the FAOD; and correlating the measured amount of the redox reaction product with an amount of the glycated albumin in the sample.