Patent ID: 6821758
Filing Date: 2004-11-23
Classification: C12N

Abstract:
A method for the production of polynucleotide molecules with modified properties, wherein at least one cycle comprising the following steps is completed:(a) providing a population of single-stranded polynucleotide molecules, wherein individual single-stranded polynucleotide molecules comprise homologous and heterologous sequence seqments and wherein individual ones of said single-stranded polynucleotide molecules can form double-stranded polynucleotide molecules with other ones of said single-stranded polynucleotide molecules within said population; (b) forming double-stranded polynucleotide molecules from the population of single-stranded polynucleotide molecules provided according to step (a) comprising double-stranded polynucleotide molecules with different heterologous sequence segments; (c) partially and exonucleolytically degrading the single-strands of the double-stranded polynucleotide molecules produced according to step (b); and (d) template-directed single-strand synthesizing the degraded ends of the partially degraded double strand produced according to step (c), wherein steps (c) and (d) may be carried out sequentially or contemporaneously.