Patent ID: 6451525
Filing Date: 2002-09-17
Classification: C12Q

Abstract:
A method for obtaining sequence information from a plurality of target polynucleotides in a sample, the method comprising:(a) contacting a plurality of different sequence primers with a polynucleotide sample under conditions effective for said primers to anneal to primer-complementary regions in one or more target polynucleotides, to form one or more target-primer hybrid(s), wherein each different-sequence primer contains (i) a target binding segment and (ii) a tag segment having a nucleotide sequence that uniquely identifies the target binding segment, (b) contacting said hybrid(s) with a labeled nucleotide terminator in the presence of a primer-extending reagent, under conditions effective to append said terminator to an end of the annealed primer in said hybrid only when said terminator is complementary to a base in the target polynucleotide that is immediately adjacent said end of the annealed primer, to form an extended hybrid mixture, (c) immobilizing said primers from the extended hybrid mixture on an addressable array of immobilized, different tag complements wherein each different tag complement contains a sequence that is complementary to said primer tag segments, under conditions effective to hybridize said primers to corresponding tag complements, and (d) identifying a nucleotide terminator appended to at least one extended primer immobilized on the array, thereby obtaining sequence information for at least one target sequence in the sample.