Patent ID: 7989208
Filing Date: 2011-08-02
Classification: G01N

Abstract:
1. A method of identifying preferred doses of a test compound known or suspected to modulate Notch processing in vivo mediated by γ-secretase comprising: (a) determining a baseline amount of Trefoil factor-3 (TFF-3) in a sample acquired from a query subject in the absence of a test compound; (b) administering a first dose of the test compound to the query subject, wherein said test compound is a known γ-secretase modulator or a suspected γ-secretase modulator; (c) determining a TFF-3 amount and a Notch-related toxicity level in a sample acquired from the query subject of step (b); (d) repeating step (b) for a plurality of different test compound doses; (e) identifying preferred doses of a compound known or suspected to modulate Notch processing mediated by γ-secretase by comparing the TFF-3 baseline amount and the TFF-3 amounts after administration of each dose of test compound, wherein doses at which there is a higher change in the amount of TFF-3 relative to the TFF-3 baseline amount are not preferred and wherein doses at which there is a lower change in the amount of TFF-3 relative to the TFF-3 baseline amount are preferred in order to minimize Notch-related toxicity levels.