Patent ID: 8597899
Filing Date: 2013-12-03
Classification: G01N,G06K,G06T,Y10S

Abstract:
1. A method of producing a cellular systems biology profile that characterizes the medical state of one or more isolated tissue samples, the method comprising: a) labeling at least one section from one or more isolated tissue samples with a histological stain, thereby producing a histologically stained section; b) labeling at least one other section from the one or more isolated tissue samples with a panel of fluorescently labeled reagents, thereby producing a fluorescently labeled section, wherein each fluorescently labeled reagent is specific for a biomarker, and wherein the panel of fluorescently labeled reagents detects at least four different biomarkers, and wherein the detection of a biomarker is a read-out of one or more features of a cellular systems biology profile; c) imaging the histologically stained section with at least a first optical mode, wherein the imaging produces a first set of data; d) imaging the fluorescently labeled section with at least a second optical mode, wherein the imaging produces a second set of data; e) analyzing the first set of data and the second set of data to identify five or more features, wherein at least one feature is identified in each of the first set of data and the second set of data; and f) calculating the interrelationships of the five or more features, wherein the interrelationships of the five or more features generates a cellular systems biology profile that characterizes the medical state of the one or more isolated tissue samples, wherein at least the steps of d) to f) are automated; thereby providing a method of producing a cellular systems biology profile that characterizes the medical state of the one or more isolated tissue samples.