Patent ID: 8742081
Filing Date: 2014-06-03
Classification: C07K,G01N,Y10T

Abstract:
1. An isolated or purified nucleic acid molecule encoding a polypeptide having a fluorescence emission activity with a maximum emission at 505 to 515 nm, wherein said nucleic acid molecule is selected from the group consisting of (a) a nucleic acid encoding a polypeptide SEQ ID NO: 2; (b) a nucleic acid having the DNA sequence of SEQ ID NO: 1; and (c) a nucleic acid that hybridizes under stringent conditions to the complementary strand of (d) a nucleic acid that hybridizes under stringent conditions to the complementary strand of the nucleic acid of (b), and wherein said nucleic acid of (d) has at positions 438 to 440 of SEQ ID NO: 1 a nucleotide triplet selected from the group consisting of TTT and TTC; and at positions 609 to 611 of SEQ ID NO: 1 a nucleotide triplet selected from the group consisting of ACT, ACC, ACA, ACG; and wherein the nucleic acid molecule encodes a polypeptide that has a fluorescence emission activity with a maximum emission at 505 to 515 nm and has a fluorescence enhanced by at least the factor of 2.5as compared to the polypeptide having the amino acid sequence of SEQ ID NO: 10.