Patent ID: 8338172
Filing Date: 2012-12-25
Classification: A61K,C07K

Abstract:
1. A method for immortalizing a population of cells that secrete antibodies of one or more specific isotypes comprising the following steps in sequence: Selecting the population of cells that expresses antibodies from one or more biological samples in an antigen-independent manner and on the basis of expression of at least a cell surface marker; Stimulating said population of selected cells with at least a stimulating agent in cell culture conditions; Eliminating said stimulating agent from the cell culture; Selecting the population of stimulated cells that expresses antibodies of one or more isotypes from said cell culture; Exposing said population of selected and stimulated cells to an immortalizing agent in cell culture conditions; Eliminating said immortalizing agent from said cell culture; Wherein the immortalizing agent is a viral immortalizing agent.