Patent ID: 8383353
Filing Date: 2013-02-26
Classification: C12Q,G01N

Abstract:
1. In vitro method for detection and/or quantification and/or identification of bacteria present in a fluid medium M constituting a biological material, a method in which, in a known manner, a suspension of microbeads in a liquid suspension medium is prepared, said microbeads being delimited by an outer surface constituted by a solid polymer material capable of binding proteins, characterized by the fact that it comprises the following stages: a) in an appropriate buffer, a loading of the microbeads in the suspension with β2GPI proteins is ensured by coupling with a sufficient quantity of β2GPI proteins, either passively in a suspension medium or using a known chemical binding protocol; b) in a container, said microbeads loaded with β2GPI proteins are brought into contact with the fluid medium M under appropriate conditions in order to ensure, without the presence of oxidizing metal ions, sufficient binding of the bacteria to the β2GPI proteins carried by the microbeads; c) the microbeads thus prepared are separated from their suspension medium, said suspension medium is removed from the container in order to obtain a residue with a high concentration of bacteria; d) and the bacteria are detected and/or quantified and/or identified, starting from the concentrated residue thus obtained.