Patent ID: 8785125
Filing Date: 2014-07-22
Classification: C07K,C12N,C12Q

Abstract:
1. A multiplex method of detecting the presence of type A1 and type C1 human papillomavirus (HPV) nucleic acids present in a biological sample, comprising the steps of: (a) treating a biological sample suspected of containing HPV type A1 or HPV type C1 to release intracellular components; (b) separating an HPV nucleic acid away from a component of the biological sample; (c) contacting the separated HPV nucleic acid with a combination of amplification oligonucleotides that amplify a HPV type A1 target sequences and a combination of amplification oligomers that amplify HPV type C1 target sequences in an E6/E7 target region sequence, wherein the combination of amplification oligomers configured to amplify HPV type A1 comprises amplification oligomers comprising nucleic acid sequences consisting essentially of SEQ ID NOS:18, 20, 22, and 38; (d) amplifying a HPV target sequence by using the amplification oligonucleotides and a nucleic acid polymerase in vitro to produce an HPV amplified product; and (e) detecting the amplified product to indicate the presence in the sample of at least one of HPV types A1 or C1.