Patent ID: 8871995
Filing Date: 2014-10-28
Classification: A61K,A61P,G01N

Abstract:
1. A method for screening an agent being useful for the treatment of dry eye and/or corneal and conjunctival lesion of dry eye severity level 3 or more according to the table below: wherein said method comprises preparing a model rabbit of corneal and conjunctival lesion having corneal and conjunctival epithelial abrasion by instilling n-heptanol solution to an eye of a rabbit such that the n-heptanol solution spreads over cornea and bulbar conjunctiva, and forcing the rabbit to blink; and administering a test agent to the eye of the model rabbit and determining an effect of restoring corneal tissue of the test agent by (i) staining the eye with a dye to show corneal lesion and (ii) observing a change in amount of staining in the eye over time, wherein an agent that is useful for said treatment reduces the amount of staining in the eye over time, wherein the preparing step further comprises forcing the rabbit to close the eye for about 1 to about 3 minutes.