Patent ID: 7927868
Filing Date: 2011-04-19
Classification: C12Q,G01N

Abstract:
1. A method for determination of one or more analytes by luminescence in one or more samples on at least five, laterally separated measurement areas, comprising the following steps: providing a device comprising a planar optical waveguide with a plurality of waveguiding regions, as part of a sensor platform, and, connected to said platform directly or by means of a sealing medium, a layer (g), said layer forming either directly a tight seal or by means of the sealing medium a tightly sealing layer, said device comprising a multitude of recesses at least open towards the sensor platform, which form a corresponding multitude of sample compartments in a 2-dimensional arrangement, wherein each of said sample compartments comprises only a single waveguide region and different biological or biochemical recognition elements, for the specific recognition and binding of different analytes, are immobilized in five or more discrete measurement areas (d) in a two-dimensional array on said single waveguiding regions in these sample compartments, wherein said measurement areas are in optical interaction with the excitation light emanating from said optical waveguide, as part of a sensor platform which forms a demarcation of said sample compartments, supplying the sample into a sample compartment and optionally reagent solutions wherein said sample compartments are operable to be cleared from received sample or reagent solutions and to receive, in the following, optionally without washing steps, further sample or reagent solutions, which are supplied to the same said sample compartments, directing excitation light the measurement areas thereby exciting substances capable of luminescence in the samples or on the measurement areas to emit luminescence, and measuring the emanated luminescence, to thereby determine one or more analytes.