Patent ID: 8691522
Filing Date: 2014-04-08
Classification: A61P,G01N

Abstract:
1. A method of determining quality of indoor air by determining if indoor air contains a material originating from at least one of pre-identified pathogenic microorganisms, the method comprising: collecting an indoor dust sample; processing the indoor dust sample to obtain a concentrated population of extracellular vesicles contained therein; mixing at least part of the concentrated population with a primer that has a sequence complementary to a first genetic substance of a first one of the pre-identified pathogenic microorganisms such that if the concentrated population contains extracellular vesicles of the first pre-identified pathogenic microorganism, the primer would recognize the first genetic substance of the first pre-identified pathogenic microorganism; subjecting the mixture to amplification of at least one specific nucleotide sequence that is recognized by the primer; processing the amplification product to determine if the at least one specific nucleotide sequence has been amplified therein; and once determined that the at least one specific nucleotide sequence has been amplified, determining that the indoor air contains materials from the first pre-identified pathogenic microorganism.