Patent ID: 6994969
Filing Date: 2006-02-07
Classification: C12Q

Abstract:
1. A method for sequencing one or more target nucleic acids present in one or more biological samples, said method comprising the steps of: (a) deriving from one or more biological samples the one or more target nucleic acids; (b) subjecting the one or more target nucleic acids obtained from step (a) to two or more separate base-specific, sequence-specific or site-specific complementary cleavage reactions, wherein each cleavage reaction generates a non-ordered set of fragments; (c) analyzing the sets of non-ordered fragments obtained from step (b) by mass spectrometry; and, (d) performing a systematic computational analysis on the mass spectra obtained from step (c) and sequencing said target nucleic acid; wherein said complementary cleavage reactions refer to comprise target nucleic acid digestions characterized by varying specificity and/or to digestion of alternative forms of the target sequence.