Patent ID: 6180406
Filing Date: 2001-01-30
Classification: C07K,C12N,C12Q,C12Y,C40B,H01L

Abstract:
A method for producing a polynucleotide having a desired functional property, said method comprising:(a) providing a population of homologous polynucleotides, wherein at least two of the related polynucleotides differ from each other by the presence of at least one deletion or insertion;(b) shuffling the homologous polynucleotides to produce a plurality of recombinant polynucleotides, and selecting or screening the population of recombinant polynucleotides to obtain at least one recombinant polynucleotide that has evolved toward the desired functional property; and,(c) shuffling the recombinant polynucleotide(s) produced in the previous step, and selecting or screening for at least one recombinant polynucleotide that has evolved toward the desired functional property.