Patent ID: 6037446
Filing Date: 2000-03-14
Classification: A61K,C07K,Y10S

Abstract:
A protein extract having antiproliferative properties prepared by a method comprising the following steps:(a) solubilizing a mixture of (i) visceral organs or neural organs or a mixture of visceral and neural organs collected from a mammalian embryo and (ii) a physiologically compatible solution;(b) centrifuging the solubilized organs to produce a pellet and a supernatant and collecting the supernatant;(c) selecting material from the supernatant that has a molecular weight less than about 10,000 daltons;(d) subjecting the material to chromatographic separation and collecting fractions of the separated material; and(e) testing the ability of a fraction to inhibit the cell proliferation, thereby identifying that fraction or fractions which have antiproliferative properties;wherein the protein extract inhibits the proliferation of a MCF-7 cell in culture.