Patent ID: 6090572
Filing Date: 2000-07-18
Classification: B01D,C12M,Y10S

Abstract:
A method for filtering a biological fluid having particulate matter and liquid and extracting one or more analytes from the particulate matter, comprising:providing a filtration and extraction device, comprising:a pliant body having an open top end and an internal wall defining an inner chamber; a sealing mechanism adapted to seal the open top end of the body; a gradient filter assembly including at least one filter; a support assembly carried by the body, the gradient filter assembly supported by the support assembly;adding the biological fluid to the chamber through the open top end;sealing and closing the open top end with the sealing mechanism;causing biological fluid to flow through the filter assembly so that the particulate matter is retained by the filter assembly and the liquid is expressed from the device by squeezing the pliant body so that a positive pressure is imparted to the chamber;unsealing and opening the open top end of the body;adding at least one reagent to the chamber though the open top end; andcausing at least one reagent to flow through said filter assembly so that the one or more analytes from the particulate matter are extracted by the at least one reagent and expressed therewith from the device for a further diagnostic assay method by squeezing the pliant body so that a positive pressure is imparted to the chamber.