Patent ID: 6235468
Filing Date: 2001-05-22
Classification: C12Q

Abstract:
A method of characterising a test sample of genomic DNA which comprisescontacting the test sample with at least one type specific primer to prime selectively, within a telomere repeat array, internal repeat units of one type, andextending the type specific primer in the presence of appropriate nucleoside triphosphates and an agent for polymerisation to produce a set of amplification products extending from the internal repeat units of that one type to at least the end of the telomere repeat array, and determining genetic variation of the telomere repeat array by the sizes of the set of amplification products;wherein a type specific primer comprises at least one sequence selected from the group consisting ofTelH (SEQ ID NO:12) 5'-CCCTAACCCTAACCCTAACCCTA-3',TelG (SEQ ID NO:13) 5'-CCCTCACCCTCACCCTCACCCTC-3',TelW (SEQ ID NO:14) 5'-CCCTTACCCTTACCCTNACCCTA-3',TelX (SEQ ID NO:15) 5'-CCCTTACCCTTACCCTNACCCTC-3', andTelY (SEQ ID NO:16) 5'-CCCTTACCCTTACCCTNACCCTG-3';thereby characterising the test sample of genomic DNA.