Patent ID: 7074584
Filing Date: 2006-07-11
Classification: C12N,C12Q

Abstract:
1. A method for generating an output array of multiple diploid yeast strains, wherein each diploid yeast strain carries at least two genetic alterations relative to a starting yeast strain, and wherein the combination of the at least two genetic alterations is different in each diploid yeast strain, the method comprising: a) generating multiple derivative yeast strains from a starting yeast strain, each derivative yeast strain carrying a starting genetic alteration relative to the starting yeast strain and wherein at least one derivative yeast strain contains a recombinant construct comprising a mating type specific promoter operably linked to a selectable marker which permits efficient recovery of haploid spore progeny and permits selection of a particular haploid mating type; b) mating sets of two derivative yeast strains of step (a) to provide resulting diploid yeast strains, wherein each of the two derivative yeast strains mated contains a starting genetic alteration relative to the starting yeast strain, which starting genetic alteration is different in each of the two mated derivative yeast strains; c) recovering multiple resulting diploid yeast strains from the mating of step (b), wherein each resulting diploid yeast strain carries a resulting genetic alteration, said resulting genetic alteration comprising the starting genetic alterations from each of the two derivative yeast strains mated in step (b); and d) arraying the resulting diploid yeast strains recovered in step (c) carrying the resulting genetic alterations as a diploid output array, wherein the combination of the starting genetic alterations comprised by said resulting genetic alteration is different in each of the arrayed resulting diploid yeast strain.