Patent ID: 8697853
Filing Date: 2014-04-15
Classification: A61P,C12N,C12Y

Abstract:
1. A nucleic acid molecule encoding a first transcription activator-like (TAL) effector endonuclease monomer, wherein the first TAL effector endonuclease monomer comprises: (i) a FokI endonuclease domain, and (ii) a TAL effector domain comprising a plurality of TAL effector repeat sequences, wherein the plurality of TAL effector repeat sequences of the first TAL effector endonuclease monomer, in combination, bind to a first nucleotide sequence in a target DNA sequence in a cell, wherein the first TAL effector endonuclease monomer is capable of forming a dimer with a second TAL effector endonuclease monomer comprising a FokI endonuclease domain and a TAL effector domain having a plurality of TAL effector repeat sequences that, in combination, bind to a second nucleotide sequence in the target DNA sequence, wherein the dimer is formed between the FokI domain of the first TAL effector endonuclease monomer and the FokI domain of the second TAL effector endonuclease monomer when the TAL effector domain of the first TAL effector endonuclease monomer is bound to the first nucleotide sequence and the TAL effector domain of the second TAL effector endonuclease monomer is bound to the second nucleotide sequence, wherein the first nucleotide sequence and the second nucleotide sequence are different and are separated by a spacer sequence, and wherein the dimer cleaves the target DNA sequence within the cell.