Patent ID: 6010873
Filing Date: 2000-01-04
Classification: A01K,A61K,A61P,C07K

Abstract:
An isolated DNA molecule which encodes a complement regulating protein analog selected from the group consisting ofcomplement receptor 1,complement receptor 2,decay accelerating factor,membrane cofactor protein,C4 binding protein, andfactor H,these complement regulating proteins wherein the carboxy terminus is removed to allow the protein to be secreted,these complement regulating proteins which bind C3b, C4b, or C3b and C4b, modified to contain short consensus repeats from a second, different complement regulating protein;these complement regulating proteins which bind C3b, C4b, or C3b and C4b wherein the short consensus repeats are rearranged, andthese complement regulating proteins which bind C3b, C4b, or C3b and C4b comprising three short consensus repeats,wherein the complement regulating protein analog contains defined amino acid substitutions in the short consensus repeats, which are selected from the group consisting ofthe complement regulating protein wherein amino acids corresponding to amino acids 114-121 of CR1 are modified in a short consensus repeat to comprise the amino acid sequence S-T-(K/R)-P-P-(I/K/V)-C-(Q/N) (SEQ ID NO:8);the complement regulating protein wherein the amino acids corresponding to positions 114-121 of CR1 are modified to contain the sequence S-T-K-P-P-I-C-Q (SEQ ID NO: 1);the complement regulating protein wherein amino acids corresponding to positions 114-121 of CR1 are modified in a short consensus repeat to contain the sequence (D/E)-(N/Q)-(E/D)-T-P-(I/L/V)-C-(D/E) (SEQ ID NO:9);the complement regulating protein wherein amino acids corresponding to amino acids 114-121 of CR1 comprise the sequence D-N-E-T-P-I-C-D (SEQ ID NO:2);the complement regulating protein wherein an amino acid corresponding to an amino acid selected from the group consisting of 35, 64, 65, and 94 of CR1 is selected from the group consisting of (G/A) 35, (R/K) 64, (N/Q) 65, and (Y/F) 94;the complement regulating protein wherein the amino acid corresponding to amino acid 92 of CR1 is T; andthe complement regulating protein wherein an amino acid selected from the group consisting of 35, 64, 65, 92 and 94, of CR1 is substituted by a different amino acid residue than the corresponding amino acid of the naturally occurring CR1.