Patent ID: 8741340
Filing Date: 2014-06-03
Classification: A61K,A61P,C12N

Abstract:
1. A monoclonal cell line comprising a transposon, said transposon comprising two terminal inverted repeats and a heterologous expression construct coding for a structural gene either i coding for a secreted biologically active polypeptide or ii coding for a polypeptide or an siRNA contributing to the generation in the cells of a biologically active secreted compound; said gene being located between said two terminal inverted repeats which are substrates for a transposase and said transposon being inserted into the genome of said monoclonal cell line using said transposase, wherein said monoclonal cell line maintains a high and stable expression level of the polypeptide or siRNA of I or ii absent the presence of a selective pressure compared to the same monoclonal cell line generated without a transposase.