Patent ID: 9133487
Filing Date: 2015-09-15
Classification: C12P

Abstract:
1. A non-naturally occurring bacterial organism having a methacrylic acid pathway, said bacterial organism comprising at least one exogenous nucleic acid encoding a methacrylic acid pathway enzyme expressed in a sufficient amount to produce methacrylic acid, said methacrylic acid pathway comprising a pathway selected from: (a) citramalate synthase, citraconate-forming citramalate dehydratase, and a decarboxylase of a C1-C6 carboxylate; (b) citramalate synthase, citraconate-forming citramalate dehydratase, citraconate isomerase, and a decarboxylase of a C1-C6 carboxylate; (c) citramalate synthase, mesaconate-forming citramalate dehydratase, citraconate isomerase, and a decarboxylase of a C1-C6 carboxylate; (d) citramalate synthase, mesaconate-forming citramalate dehydratase, and a decarboxylase of a C1-C6 carboxylate; (e) citramalyl-CoA lyase, citramalyl-CoA transferase, synthetase or hydrolase, citraconate-forming citramalate dehydratase and a decarboxylase of a C1-C6 carboxylate; (f) citramalyl-CoA lyase, citramalyl-CoA transferase, synthetase or hydrolase, citraconate-forming citramalate dehydratase citraconate isomerase, and a decarboxylase of a C1-C6 carboxylate; (g) citramalyl-CoA lyase, citramalyl-CoA transferase, synthetase or hydrolase, mesaconate-forming citramalate dehydratase, and a decarboxylase of a C1-C6 carboxylate; (h) citramalyl-CoA lyase, citramalyl-CoA transferase, synthetase or hydrolase, mesaconate-forming citramalate dehydratase, citraconate isomerase, and a decarboxylase of a C1-C6 carboxylate; (i) aconitate decarboxylase, itaconate isomerase, and a decarboxylase of a C1-C6 carboxylate; (j) aconitate decarboxylase, itaconate isomerase, citraconate isomerase, and a decarboxylase of a C1-C6 carboxylate; (k) aconitate decarboxylase, itaconyl-CoA transferase, synthetase or hydrolase, citramalyl-CoA dehydratase, citramalyl-CoA transferase, synthetase or hydrolase, citraconate-forming citramalate dehydratase, and a decarboxylase of a C1-C6 carboxylate; (l) aconitate decarboxylase, itaconyl-CoA transferase, synthetase or hydrolase, citramalyl-CoA dehydratase, citramalyl-CoA transferase, synthetase or hydrolase, citraconate-forming citramalate dehydratase, citraconate isomerase, and a decarboxylase of a C1-C6 carboxylate; (m) aconitate decarboxylase, itaconyl-CoA transferase, synthetase or hydrolase, citramalyl-CoA dehydratase, citramalyl-CoA transferase, synthetase or hydrolase, mesaconate-forming citramalate dehydratase, and a decarboxylase of a C1-C6 carboxylate; and (n) aconitate decarboxylase, itaconyl-CoA transferase, synthetase or hydrolase, citramalyl-CoA dehydratase, citramalyl-CoA transferase, synthetase or hydrolase, mesaconate-forming citramalate dehydratase, citraconate isomerase, and a decarboxylase of a C1-C6 carboxylate.