Patent ID: 6133416
Filing Date: 2000-10-17
Classification: A01K,C07K,C12N,C12Y

Abstract:
A method for preparing a composition comprising a factor having the following characteristics:a) resistant to protease;b) resistant to nuclease;c) resistant to heating at 100.degree. C. for 10 minutes;d) resistant to freezing-thaw;e) resistant to lyophilization;f) pH stable; andg) has an apparent molecular weight of between about 0.5 and 1 kDa;h) has a biological activity of inhibiting the proliferation of a tumor cell;wherein the method comprises the steps of:i) contacting a cell with a transcriptionally active expression vector which expresses a tumor suppressor gene;ii) culturing said cell in cell culture media under conditions where expression from said vector occurs; andiii) purifying said anti-proliferative factor by subjecting said culture media to size fractionation.