Patent ID: 7074772
Filing Date: 2006-07-11
Classification: A61K,C12N,Y10S

Abstract:
1. A method for delivering a DNA construct into a host cell for transcription of RNA molecules, said construct comprising a circular strand of DNA which forms a dumbbell, said circular strand comprising a first complementary sequence, a first non-complementary sequence, a second complementary sequence and a second non-complementary sequence, wherein said first and second complementary sequences pair to form a duplex in said dumbbell and said duplex comprises a) a promoter sequence, b) a coding sequence, and c) a polyadenylation sequence or other stabilizing sequence, and wherein said first and second non-complementary sequences form single-strand loops in said dumbbell, said method comprising the steps of: directly administering said DNA construct into the host cell; and transcribing RNA molecules in the host cell.