Patent ID: 9109217
Filing Date: 2015-08-18
Classification: A61K,A61P,C12N,C12Y

Abstract:
1. A method of reducing reactivity of ADAMTS-13 (a disintegrin-like domain, and metalloprotease) to an anti-ADAMTS-13 neutralizing antibody, with an isolated human thrombospondin type 1 motif, member 13, the method comprising substituting the following positions in the isolated human ADAMTS-13 with a different charged or uncharged amino acid: arginine at position 326, aspartic acid at position 330, aspartic acid at position 343 and arginine at position 349 in the disintegrin-like domain, aspartic acid at position 480, arginine at position 488, arginine at position 498, arginine at position 507, aspartic acid at position 533 and aspartic acid at position 543 in the cysteine-rich domain, and glutamic acid at position 641 and arginine at position 66o in the spacer domain, in the amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1.