Patent ID: 8586363
Filing Date: 2013-11-19
Classification: A61P,C12N,C12Y

Abstract:
1. A method for modifying the genetic material of a cell, comprising: (a) providing a cell containing a target DNA sequence; and (b) introducing a first transcription activator-like (TAL) effector endonuclease monomer and a second TAL effector endonuclease monomer into the cell, each TAL effector endonuclease monomer comprising: such that the FokI endonuclease domain of the first TAL effector endonuclease monomer and the FokI endonuclease domain of the second TAL effector endonuclease monomer form a dimer that cleaves the target DNA sequence within the cell or progeny thereof when the TAL effector domain of the first TAL effector endonuclease monomer is bound to the first specific nucleotide sequence and the TAL effector domain of the second TAL effector endonuclease monomer is bound to the second specific nucleotide sequence.