Patent ID: 7741042
Filing Date: 2010-06-22
Classification: B01J,C12Q

Abstract:
1. A method for producing a DNA chip, the method comprising the steps of: (a) ligating one or more double-helical probes into one or more vectors, wherein each probe comprises an oligonucleotide coupled to a linker at one or both ends, and wherein the oligonucleotide is a 60 bp by sequence specific to an HPV genotype, where a linker is coupled to both ends of an oligonucleotide to be integrated on a slide, into a vector; (b) transforming host cells with the vectors; (c) culturing the transformed host cells to recover a plurality of double-helical probes, each including the linker, wherein the linker is a part of the base sequence of the vector; and (d) integrating the plurality of double-helical probes on a slide to produce a DNA chip, wherein the double-helical probes of said plurality of double helical probes are selected from the group consisting of: (i) SEQ ID NOs: 1 and 2; (ii) SEQ ID NOs: 3 and 4; (iii) SEQ ID NOs: 5 and 6; (iv) SEQ ID NOs: 7 and 8; (v) SEQ ID NOs: 9 and 10; (vi) SEQ ID NOs: 11 and 12; (vii) SEQ ID NOs: 13 and 14; (viii) SEQ ID NOs: 15 and 16; (ix) SEQ ID NOs: 17 and 18; (x) SEQ ID NOs: 19 and 20; (xii) SEQ ID NOs: 21 and 22; (xiii) SEQ ID NOs: 23 and 24; (xiv) SEQ ID NOs: 25 and 26; (xv) SEQ ID NOs: 27 and 28; (xvi) SEQ ID NOs: 29 and 30; (xvii) SEQ ID NOs: 31 and 32; (xviii) SEQ ID NOs: 33 and 34; (xix) SEQ ID NOs: 35 and 36; (xx) SEQ ID NOs: 37 and 38; (xxi) SEQ ID NOs: 39 and 40; (xxii) SEQ ID NOs: 41 and 42; (xxiii) SEQ ID NOs: 43 and 44; (xxiv) SEQ ID NOs: 45 and 46; (xxv) SEQ ID NOs: 47 and 48; (xxvi) SEQ ID NOs: 49 and 50; (xxvii) SEQ ID NOs: 51 and 52; and (xxviii) SEQ ID NOs: 53 and 54; and wherein the DNA chip is effective in the identification of Human Papilloma Virus (HPV) infection and the genotype of HPV infection in a sample.