Patent ID: 7238854
Filing Date: 2007-07-03
Classification: C12N

Abstract:
1. An inactive recombinase element selected from the group consisting of: a) a DNA construct having the general structure in a 5′ to 3′ orientation comprising: P1-ssrN-IntN, wherein: wherein each of P1, ssrN, and IntN are operably linked such that activation of the promoter results in the expression of the ssrN-IntN fusion protein lacking recombinase activity; and b) a DNA construct having the general structure in a 5′ to 3′ orientation comprising: P2-IntC-ssrC, wherein: wherein each of P2, ssrC, and IntC are operably linked such that activation of the promoter results in the expression of the IntC-ssrC fusion protein lacking recombinase activity, and wherein the inactive recombinase elements of (a) and (b) when present together in a cell will form an active recombinase protein by intein-mediated trans-protein splicing.