Patent ID: 8993243
Filing Date: 2015-03-31
Classification: G01N

Abstract:
1. A method for isolating weakly interacting molecules from a fluidic sample, the method comprising: a) forming a mixture comprising the fluidic sample and a solid phase substrate, wherein the solid phase substrate comprises at least one immobilized molecule thereon; b) incubating the mixture under conditions sufficient for the immobilized molecule to interact with a target molecule in the fluidic sample to form a solid phase substrate immobilized molecule-target molecule complex, wherein the immobilized molecule and the target molecule have an interaction half-life of about 10 minutes or less; and c) transferring the solid phase substrate-immobilized molecule-target molecule complex into an immiscible phase in about 1 second or less; wherein the forming and the incubating are performed in an input zone of a device comprising: i) the input zone; and ii) a zone comprising the immiscible phase; wherein a first constriction separates the input zone from the zone comprising the immiscible phase.