Patent ID: 7371517
Filing Date: 2008-05-13
Classification: A61D,B03B,C12N,C12Q,G01F,G01N

Abstract:
1. A method of isolating X-chromosome bearing sperm cells and Y-chromosome bearing sperm cells, comprising the steps of: collecting viable sperm cells from a male of a species of non-human mammal; staining said viable sperm cells to produce viable, stained sperm cells; differentiating between X-chromosome bearing sperm cells and Y-chromosome bearing sperm cells based upon fluorescent light emitted from said viable, stained sperm cells which is detected with a photomultiplier tube operating at below 400 volts; separating said X-chromosome bearing sperm cells and Y-chromosome bearing sperm cells into a X-chromosome bearing population and a Y-chromosome bearing population at a separation rate between 4,000 to 10,000 separations per second of each sex; and producing separate X-chromosome bearing and Y-chromosome bearing populations of sperm cells each having a purity of greater than 90%.