Patent ID: 9175337
Filing Date: 2015-11-03
Classification: C12P,C12Q

Abstract:
1. A method of amplifying a nucleic acid target region, the method comprising: contacting a target nucleic acid comprising the target region with (1) a first amplification oligomer comprising (2) a second amplification oligomer comprising a target-binding segment T2 complementary to a region of the first amplicon that is the complement of a 5′-end of the target region, and wherein said contacting further comprises conditions whereby the first amplicon serves as a template to produce a second amplicon comprising segments cT1 and cD1, complementary to T1 and D1, respectively; (3) a third amplification oligomer comprising target-binding priming segment T1 (4) a fourth amplification oligomer comprising a displacer priming segment D1 wherein said contacting further comprises conditions whereby the second amplicon serves as a template for extension from both the third and fourth amplification oligomers, wherein extension of T1