Patent ID: 6455263
Filing Date: 2002-09-24
Classification: B01J,C40B,G01N

Abstract:
A method for screening bioactive agents which bind to a target molecule, while reducing the incidence of false positives, said method comprising:(a) providing a population of one species of target molecule comprising at least a first subpopulation labeled with a first fluorescent moiety and a second subpopulation labeled with a second fluorescent moiety, wherein the target molecules in the first and second subpopulations are the same; (b) contacting a library of candidate bioactive agents covalently attached to a plurality of beads with said population of target molecules under reaction conditions that favor binding of the target molecule to a member of said library of bioactive agents; and (c) sorting the beads from step (b) using a fluorescent activated cell sorter (FACS) machine to obtain a fluorescent population of beads and a non-fluorescent population of beads, wherein the beads in said fluorescent population have said first and second fluorescent moieties bound thereto, thereby indicating the binding of said target molecule to at least one member of said library of candidate bioactive agents.