Patent ID: 6974669
Filing Date: 2005-12-13
Classification: B01J,B82Y,C07B,C07H,C12Q,C40B,Y10S

Abstract:
1. A method for detecting for the presence or absence of a target analyte in a sample comprising: providing a particle complex probe comprising a particle having oligonucleotides bound thereto, a DNA barcode, and a oligonucleotide having bound thereto a specific binding complement to a target analyte, wherein the DNA barcode has a sequence having at least two portions, at least some of the oligonucleotides attached to the particle have a sequence that is complementary to a first portion of a DNA barcode, the oligonucleotides having bound thereto a specific binding complement have a sequence that is complementary to a second portion of a DNA barcode, and wherein the DNA barcode is hybridized at least to some of the oligonucleotides attached to the particle and to the oligonucleotides having bound thereto the specific binding complement; contacting the sample with a particle complex probe under conditions effective to allow specific binding interactions between the analyte and the particle complex probe and form an aggregated complex in the presence of the analyte; isolating the aggregated complex and subjecting the aggregated complex to conditions effective to dehybridize the aggregated complex and to release the DNA barcode; isolating the DNA barcode; and detecting for the presence of DNA barcode, wherein the presence of the DNA barcode is indicative of the presence of the target analyte in the sample.