Patent ID: 8057990
Filing Date: 2011-11-15
Classification: A61K,A61P,G01N

Abstract:
1. A method for screening a compound or a salt thereof that alters a cell stimulating activity, comprising: measuring an intracellular calcium concentration increase activity of a GPR30 protein, (i) when a tacrine compound is brought in contact with the protein in a cell in the absence of a test compound, and (ii) when a tacrine compound is brought in contact with the protein in a cell in the presence of the test compound, wherein said GPR30 protein comprises the amino acid sequence represented by SEQ ID NO: 1 or an amino acid sequence having at least 80% homology to the amino acid sequence represented by SEQ ID NO: 1; comparing the intracellular calcium concentration increase activity between (i) and (ii); and selecting the compound that alters the intracellular calcium concentration increase activity; (in the formula, ring A represents an optionally substituted benzene; R each of R R or a salt thereof.