Patent ID: 8497093
Filing Date: 2013-07-30
Classification: C07K

Abstract:
1. A method of modulating the expression of a gene in a host cell comprising the gene to be modulated, the method comprising: (a) introducing into the host cell a gene expression modulation system comprising (b) introducing into the host cell a ligand that binds to the leafhopper ecdysone receptor ligand binding domain, wherein one of the first gene expression cassette or the second gene expression cassette comprises a polynucleotide sequence encoding a DNA-binding domain that recognizes a response element associated with a gene of interest, wherein the first gene expression cassette or the second gene expression cassette that does not comprise a polynucleotide sequence encoding the DNA-binding domain comprises a polynucleotide sequence encoding a transactivation domain, wherein the ligand binding domain in the first polypeptide and the ligand binding domain in the second polypeptide are different and dimerize, and wherein the gene that is expressed is a component of a chimeric gene comprising: (i) a response element to which the DNA-binding domain binds; (ii) a promoter that is activated by the transactivation domain; and (iii) the gene that is expressed.