Patent ID: 6356845
Filing Date: 2002-03-12
Classification: A61P,C07K,C12N

Abstract:
A method for crystallizing an Staphylococcus aureus UDP-N-acetylenolpyruvylglucosamine reductase molecule or molecular complex comprising:preparing purified S. aureus MurB at a concentration of about 1 mg/ml to about 50 mg/ml; and crystallizing S. aureus MurB from a solution comprising about 1 wt. % to about 50 wt. % PEG, 0 wt. % to about 40 wt. % DMSO, about 100 mM to about 1 M ammonium or lithium sulfate, about 0 mM to about 20 mM 2-mercaptoethanol, about 0.005 mM to about 40 mM EP-UDPGlcNAc, and buffered to a pH of about 5 to about 8.