Patent ID: 6074825
Filing Date: 2000-06-13
Classification: C12Q

Abstract:
A method for biologically preparing a class of stable encapsulated reference nucleic acids for molecular diagnostic and genetic testing of test cells comprising:inserting a vector selected from the group consisting of:(i) a first vector bearing a reference nucleic acid and a second identical vector bearing a second nonidentical reference nucleic acid,(ii) a first vector bearing a reference nucleic acid and a second nonidentical vector bearing a second nonidentical reference nucleic acid, and(iii) a vector bearing at least two nonidentical reference nucleic acids into a cell through a lipoprotein outer membrane of the cell to encapsulate the reference nucleic acids;propagating the reference nucleic acid by multiplying the cell number;causing cell death without affecting the encapsulated reference nucleic acid; andachieving a desired stability of the membrane by crosslinking the protein in the membrane for substantially matching with the membrane stability of the test cells.