Patent ID: 8030544
Filing Date: 2011-10-04
Classification: A01H,C12N,C12R,C12Y,G01N

Abstract:
1. A method for producing a transgenic plant containing at least two heterologous nucleic acid sequences, comprising: (a) providing an explant comprising a first heterologous nucleic acid sequence that confers resistance to an herbicide, an aminoglycoside, or hygromycin; (b) inoculating the explant with (c) co-cultivating the explant with the Agrobacterium to obtain an explant that exhibits spectinomycin resistance; and (d) regenerating the explant that exhibits spectinomycin resistance into a transgenic plant containing at least the two heterologous nucleic acid sequences; wherein the explant is treated with a cytokinin at least during steps (b) and (c).