Patent ID: 7396648
Filing Date: 2008-07-08
Classification: C12Q,Y10T

Abstract:
1. A method for discriminating a target base carried by a target DNA, comprising: (1) a DNA elongation step comprising wherein said target DNA comprises the sequence 3′-(G-G-S1)-5′, S1 being the target base, and wherein said test allele specific primer comprises the sequence 5′-(B3-B2-S1′)-3′, (B3, B2) being bases selected from the group consisting of (T, T), (T, G), (G, T), and (G, G), wherein S1′ is the 3′ terminal base of the test allele specific primer and is complimentary to the base predicted to be S1; and (2) a discrimination step comprising measuring the efficiency of said DNA elongation to discriminate that said target is the same as said predicted base when the efficiency is high, or that said target base is distinct from said predicted base that said target base is distinct from said predicted base.