Patent ID: 8354241
Filing Date: 2013-01-15
Classification: A61K,A61P,C07K,G01N

Abstract:
1. A method for identifying a GLP-1 secretagogue or a compound useful for treating a condition ameliorated by increasing a GLP-1 level, comprising the steps of: (a) contacting a test compound with an isolated host cell or with a membrane of a host cell that expresses a G protein-coupled receptor, wherein the G protein-coupled receptor comprises an amino acid sequence selected from the group consisting of: (b) determining the ability of the test compound to stimulate functionality of the G protein-coupled receptor; (c) contacting a test compound which stimulates functionality of the G protein-coupled receptor in step (b) in vitro with a mammalian enteroendocrine cell; and (d) determining whether the test compound stimulates GLP-1 secretion from the mammalian enteroendocrine cell; wherein the ability of the test compound to stimulate GLP-1 secretion from the mammalian enteroendocrine cell is indicative of the test compound being a GLP-1 secretagogue or a compound useful for treating a condition ameliorated by increasing a GLP-1 level.