Patent ID: 6027650
Filing Date: 2000-02-22
Classification: B01D,C07K

Abstract:
A vertical column chromatographic method, without the need to use a moving packed bed adapter and without column inversion the method comprising:a) contacting with a solid support a first liquid containing a sample molecule and having a first physical characteristic;b) contacting with the first liquid a second liquid having a second physical characteristic and acts as an eluent of the sample molecule;c) flowing the liquids through the solid support; andd) collecting the sample molecule in an elution pool volume;wherein the second liquid follows the first liquid and remains in contact with the first liquid;wherein the physical characteristics of the first and second liquids generate an interface between the first and second liquids and mixing is minimized between the two liquids as they flow through the solid support; andwherein the elution pool volume is less than approximately 80% of the elution pool volume of the sample molecule in a control process analysis.