Patent ID: 7947460
Filing Date: 2011-05-24
Classification: C07K,G01N

Abstract:
1. A method of diagnosing a hazelnut allergy characterized by a hazelnut-induced systemic reaction comprising: contacting an IgE-containing biological specimen of a human with an isolated and purified polypeptide that forms a complex with serum IgE from a human with said allergy, wherein said polypeptide is encoded by a first nucleic acid having the sequence of nucleotides 25-924 of SEQ ID NO:1 or a second nucleic acid that hybridizes over the full length of the complement of said first nucleic acid under high stringency conditions comprising 68° C. in aqueous buffered solution and wherein said second nucleic acid encodes a polypeptide that is recognized by antibodies specific for the polypeptide encoded by said first nucleic acid; determining the presence or absence of a complex between IgE in the specimen and the polypeptide; and diagnosing hazelnut allergy characterized by a hazelnut-induced systemic reaction if the complex is present.