Patent ID: 6699660
Filing Date: 2004-03-02
Classification: A61K,A61P,C07K,C12Q

Abstract:
An isolated nucleic acid comprising at least 60 bases in length, and that hybridizes to the sense or antisense strand of a second nucleic acid under highly stringent hybridization conditions, wherein said second nucleic acid has a sequence as set forth in SEQ ID NO:31, wherein the isolated nucleic acid is expressed in response to seizure or ischemia and wherein said hybridization conditions include 1-15 ng/mL of isolated nucleic acid probe hybridizing to said second nucleic acid bound to a nitrocellulose filter, hybridization in 25 mM KPO4 (pH 7.4), 5Ã—SSC, 5Ã—Denhart's solution, 50 &mgr;g/mL denatured sonicated salmon sperm DNA, 50% formamide, 10% Dextran sulfate at 42Â° C., with washes at 50Â° C. in 0.2Ã—SSC and 0.1% SDS.