Patent ID: 7501231
Filing Date: 2009-03-10
Classification: A01N,C12N

Abstract:
1. A cryopreservation composition for cryopreserving non-proliferative primary neuronal cells from the CNS comprising nutrient medium, serum, D-glucose, L-glutamine, an antibiotic, a cryopreservative, and three antioxidants comprising ascorbic acid, glutathione, and N-acetylcysteine, wherein the composition comprises about 20% (v/v) serum, about 2 mM glutamine, about 20 mM glucose, about 1 U/ml penicillin-streptomycin, about 400 mM ascorbic acid, about 2 mM glutathione, about 300 mM N-acetylcysteine and about 80% (v/v) dimethylsulfoxide (DMSO).