Patent ID: 9127089
Filing Date: 2015-09-08
Classification: A61K,A61P,C07K

Abstract:
1. A method for preparing highly-purified soluble thrombomodulin having a content of host cell-originated proteins being in a ratio of less than 10 ng of the proteins per 10,000 U of soluble thrombomodulin, which comprises the step of: passing a solution containing soluble thrombomodulin, which is produced by a transformant cell obtained by transfecting a host cell with a DNA containing a nucleotide sequence encoding soluble thrombomodulin, through nylon and/or polyethersulfone in the form of a membrane, non-woven fabric, or beads in order to reduce the concentration of host-cell originated proteins; and recovering high-purified soluble thrombomodulin that has passed through the nylon and/or polyethersulfone membrane, non-woven fabric, or beads; wherein the nylon and/or polyethersulfone membrane, non-woven fabric, or beads adsorbs host cell-originated proteins.