Patent ID: 8124074
Filing Date: 2012-02-28
Classification: A61K,A61P,C07K,C12N

Abstract:
1. A single chain, polypeptide fusion protein, comprising: a) a non-cytotoxic protease, or a fragment thereof, which protease or protease fragment cleaves a protein of the exocytic fusion apparatus of a target cell; b) a Targeting Moiety that binds to a Binding Site on the target cell, which Binding Site undergoes endocytosis to be incorporated into an endosome within the target cell; c) a protease cleavage site at which site the fusion protein is cleaved by a protease, wherein the protease cleavage site is located between the non-cytotoxic protease or fragment thereof and the Targeting Moiety; and d) a translocation domain that is capable of translocating the protease or protease fragment from within an endosome, across the endosomal membrane and into the cytosol of the target cell; wherein the Targeting Moiety is located between the protease cleavage site and the translocation domain.