Patent ID: 8298401
Filing Date: 2012-10-30
Classification: C12Q,G01N

Abstract:
1. A method of determining the concentration of glucose in a blood sample, wherein the blood sample is added to a disposable, electrochemical biosensor test strip comprising: providing the disposable, electrochemical biosensor test strip including a capillary chamber having a depth suitable for capillary flow of blood, and holding a volume from about 0.2 to about 0.6 μl of the blood sample, and a reagent within the capillary chamber, the reagent including an enzyme and a mediator; applying a blood sample containing glucose into the capillary chamber, the capillary chamber directing capillary flow of the blood sample into contact with the reagent to cause the blood sample to at least partially solubilize or hydrate the reagent, the glucose reacting with the reagent to produce an electroactive reaction product; detecting the blood sample in the capillary chamber; following said detecting, providing a delay period prior to electrooxidizing the electroactive reaction product; following said delay period, electrooxidizing the electroactive reaction product, said electrooxidizing producing an assay current; measuring the assay current at one or more times after the delay period, correlating the one or more assay current measurements to the concentration of glucose in the blood sample, and within a period of time from about 4 to about 6 seconds after said detecting, providing a readout of the glucose concentration in the blood sample.