Patent ID: 8871444
Filing Date: 2014-10-28
Classification: B01F,B01J,B01L,C12N,C12P,C12Q,G01N

Abstract:
1. A method for detecting a product of an enzymatic reaction, comprising the steps of: providing a droplet generator to produce, under microfluidic control, a plurality of aqueous microcapsules surrounded by an immiscible continuous phase that comprises a fluorinated oil that comprises a fluorinated polymer surfactant, each of the plurality of microcapsules comprising an enzyme, a genetic element, and reagents for the enzymatic reaction; pooling the microcapsules into one or more common compartments such that a portion of the plurality of microcapsules contact each other but do not fuse with each other due to the presence of the surfactant; conducting the enzymatic reaction on the genetic element of at least one of the plurality of microcapsules within the one or more common compartments; and detecting the product of the enzymatic reaction.