Patent ID: 7906115
Filing Date: 2011-03-15
Classification: A61K,C07K

Abstract:
1. A method of inhibiting virus replication or spread to additional host cells or tissues, comprising contacting a human cell with at least a first anti-viral agent, wherein said first anti-viral agent is an immunoconjugate that comprises one or more anti-viral drugs operatively attached to a first antibody, in an amount effective to inhibit virus replication in said cell or to inhibit spread to additional host cells or tissues from said cell; wherein said first antibody effectively competes with the 3G4 antibody (monoclonal antibody 3G4 deposited as ATCC PTA 4545) in binding to an ELISA plate coated with phosphatidylserine in a competition ELISA conducted in the presence of serum, wherein said competition ELISA comprises: (a) coating an ELISA plate with phosphatidylserine to prepare a PS-coated ELISA plate; (b) blocking said PS-coated ELISA plate with a blocking buffer comprising 10% serum; (c) adding said 3G4 antibody diluted in said blocking buffer to said PS-coated ELISA plate under conditions effective to allow binding of said 3G4 antibody to said PS-coated ELISA plate in the presence of serum; (d) detecting the binding of said 3G4 antibody to said PS-coated ELISA plate in the presence of serum using a secondary antibody that binds to said 3G4 antibody; and (e) identifying a first antibody that effectively competes with said 3G4 antibody in said competition ELISA by selecting a first antibody that substantially reduces the binding of said 3G4 antibody to said PS-coated ELISA plate in the presence of serum.