Patent ID: 8895715
Filing Date: 2014-11-25
Classification: C07K,C12N

Abstract:
1. An isolated polynucleotide comprising an S100B regulatory element having at least 95% sequence identity to SEQ ID NO:2 wherein the sequence elements at SEQ ID NO:2 residues 85-105; residues 141-160; residues 212-231; residues 317-344; residues 415-447; residues 495-514; residues 531-553; residues 562-582; residues 603-624; residues 651-710; residues 720-746; residues 806-829; residues 931-966; residues 1191-1210; residues 1245-1287; residues 1300-1341; residues 1350-1375; residues 1422-1445; residues 1548-1577; residues 1630-1658; residues 1708-1730; residues 1743-1783; residues 1786-1807; residues 1912-1938; residues 2088-2158; residues 2212-2231; and residues 2315-2341 are each conserved and present in said polynucleotide, operably joined to an S100B basal promoter having at least 95% sequence identity to SEQ iD NO:3 wherein the sequence elements at SEQ ID NO:3, residues 79-116; residues 246-285; residues 424-503; residues 562-585; residues 769-792; residues 825-844; residues 880-933; residues 949-968; residues 975-1146; residues 1154-1173; and residues 1192-1218 are each conserved and present in said polynucleotide, wherein the S100B regulatory element and the S100B basal promoter are joined through a non-native spacing of not more than 1 kb between the basal promoter and the regulatory element.