Patent ID: 6210440
Filing Date: 2001-04-03
Classification: A61F,A61L,Y10S

Abstract:
A method of preparing a ligament xenograft for implantation into a human, which comprises,a. removing at least a portion of ligament from a joint of a non-human animal to provide a xenograft;b. washing the xenograft in water and alcohol;c. subjecting the xenograft to a cellular disruption treatment;d. digesting the xenograft with a glycosidase to remove substantially a plurality of first cell surface carbohydrate moieties from the xenograft, wherein the glycosidase has a concentration in the range of about 1 mU/ml to about 1000 U/ml, ande. treating a plurality of second cell surface carbohydrate moieties on the xenograft with a plurality of sialic acid molecules to cap at least a portion of the second cell surface carbohydrate moieties, wherein the sialic acid molecules have a concentration in a range of about 0.01 mM to about 100 mM,whereby the xenograft is substantially non-immunogentic and has substantially the same mechanical properties a corresponding portion of native human ligament.