Patent ID: 6787338
Filing Date: 2004-09-07
Classification: B01L,G01N

Abstract:
A method of subjecting a sample to rapid thermal cycling, said method comprising:a) contacting a sample holder containing a sample with heated fluid, thereby raising the temperature of the sample to a first temperature, and holding the sample at about said first temperature for a first predetermined period of time; b) contacting the sample holder with non-heated fluid, thereby lowering the temperature of the sample to a second temperature, an holding the sample at about said second temperature for a second predetermined period of time; c) contacting the sample holder with heated fluid, thereby raising the temperature of the sample to a third temperature, and holding the sample at about said third temperature for a third predetermined period of time; wherein steps a) through c) are completed within a time range of about 30 seconds to 60 seconds and repeated at least one time; and wherein said sample holder has a thermal mass which provides for completing said cycle within said time range.