Patent ID: 6468739
Filing Date: 2002-10-22
Classification: C07K,C12Q

Abstract:
A method for identifying a Helicobacter pylori gene which codes for a protein having secretory activity, the method comprising the steps of:(a) providing a prokaryotic host cell which contains (1) a transposon inducible in the host cell coupled to a marker for secretory activity and (2) a library of minimal plasmid vectors comprising (i) an expression control sequence active in the host cell and (ii) a H. pylori DNA library which may contain a gene which codes for a protein having secretory activity, wherein the H. pylori DNA is operatively linked to the expression control sequence; (b) inducing the insertion of the transposon into the H. pylori DNA; and (c) identifying any H. pylori gene which codes for a protein having secretory activity, by detecting the marker.