Patent ID: 6010849
Filing Date: 2000-01-04
Classification: C07K,C12N,C12Q,H01L

Abstract:
A method of screening for a molecule which binds to a selected test sequence in a duplex DNA, comprising(i) constructing a duplex DNA test oligonucleotide having a screening sequence adjacent a selected test sequence, where a DNA binding protein is effective to bind to said screening sequence with a binding affinity that is substantially independent of such test sequence, but where binding of the DNA binding protein to the screening sequence is sensitive to binding of test molecules to such test sequence,(ii) adding a test molecule to be screened to a test system composed of (a) said DNA binding protein, and (b) said duplex DNA test oligonucleotide having said screening and test sequences adjacent one another,(iii) incubating the molecule in the test system for a period sufficient to permit binding of the molecule being tested to the test sequence in the duplex DNA, and(iv) comparing the amount of binding protein bound to the duplex DNA before and after said adding.