Patent ID: 6033866
Filing Date: 2000-03-07
Classification: C12Q,Y10S

Abstract:
An amperometric sensor for the determination of glucose in aqueous media comprising:a sensing electrode, the sensing electrode comprising;a non-conductive support member, with the non-conductive support member comprising;a non-conductive polymeric film;the non-conductive polymeric film coated with an electrically conductive layer, with the electrically conductive layer containing;a first redox mediator; anda reference electrode comprising;a non-conductive polymeric film, the polymeric film coated with an electrically conductive formation comprising Ag/AgCl dispersed in a resin formulation, and with the reference electrode having an opening; anda reagent strip, the reagent strip comprising;a carrier strip that is a porous or fibrous water absorbent matrix, the carrier strip impregnated with a mixture comprising;glucose oxidase,horseradish peroxidase,a second redox mediator that can be oxidized by hydrogen peroxide under catalysis by horseradish peroxidase,at least one surfactant,at least one stabilizer,a buffering agent to maintain a pH from about 4 to about 8;with the electrically conducting layer of the sensing electrode and the electrically conductive formulation of the reference electrode facing each other; and with the reagent strip superimposed on and in physical contact with the electrically conducting layer of the sensing electrode, and with the reference electrode superimposed on the reagent strip so that the electrically conductive formulation coating the reference electrode is superimposed on the reagent strip and in physical contact with the reagent strip; so that the sensing electrode and the reagent strip and the reference electrode form a sandwich.