Patent ID: 7531322
Filing Date: 2009-05-12
Classification: A61K,C07K,C12N

Abstract:
1. A method for producing cell-specific retroviral vectors, the method comprising (a) immunizing a mammal with one or more cell population(s), (b) isolating RNA from the immunized mammal, the RNA comprising RNA from a B cell, (c) producing, from the isolated RNA, cDNAs that encode single chain antibodies (scFv-cDNAs), (d) ligating the scFv-cDNAs into a phagemid vector, (e) transforming a host bacterium with the phagemid vector, (f) isolating phages that bind to the cell population(s), (g) excising the scFv-encoding cDNA from the phages obtained in step (f) and ligating the cDNA into a psi-negative retroviral Env expression vector, comprising an env gene, to produce an Env-scFv expression vector, (h) transforming the Env-scFv expression vector into a packaging cell, and (i) isolating the retroviral vectors secreted by the packaging cell.