Patent ID: 6872816
Filing Date: 2005-03-29
Classification: C12N,C12Q,Y10S

Abstract:
1. A kit comprising: a) a first oligonucleotide comprising a 3′ portion and a 5′ portion said 3′ portion completely complementary to a first region of a target nucleic acid wherein said 3′ portion when hybridized to said target nucleic acid forms a hybridized region and said 5′ portion non-complementary to said target nucleic acid; and b) a second oligonucleotide comprising a 3′ portion and a 5′ portion, said 5′ portion completely complementary to a second region of said target nucleic acid downstream of and contiguous to said first region wherein said 3′ portion extends into said hybridized region; and c) a structure-specific 5′-nuclease.