Patent ID: 6518040
Filing Date: 2003-02-11
Classification: C07K,C12P

Abstract:
A method of in vitro production of a protein or polypeptide wherein mRNA encoding said protein or polypeptide is translated, said method comprising:translating said mMNA when said mRNA is bound to a solid phase; wherein a) said translating is paused at one or more predetermined internal codons in the mMNA while maintaining the nascent protein or polypeptide bound in a tRNA-ribosome mRNA complex to said solid phase, and b) said translating, up to each of said one or more predetemined internal codons is performed with a translation mix devoid of one or more components necessary to read through the said one or more predetermined internal codons thereby causing said translating to become stalled, and c) said translating is restarted by adding a subsequent translation mix containing the factors necessary to read through said one or more predetermined internal codons.