Patent ID: 8361739
Filing Date: 2013-01-29
Classification: C12Q

Abstract:
1. A method of determining the effect of a test compound on a non-luminogenic enzyme activity in an assay sample not containing living cells, comprising: (a) providing a test compound and a luminogenic molecule, wherein the luminogenic molecule is both a substrate of the non-luminogenic enzyme and a pro-substrate of a luciferase enzyme; (b) contacting the test compound, the non-luminogenic enzyme, luciferase, the luminogenic molecule, and a tolerance enhancement agent, so as to produce a reaction mixture, wherein said tolerance enhancement agent is present in an amount sufficient to substantially protect the activity of the luciferase from interference from the test compound and thereby reduce the likelihood of a false positive resulting from the interfering effect of the test compound, relative to the absence of tolerance enhancement agent; (c) detecting luminescence in the reaction mixture; and (d) determining the effect of the test compound, if any, on the non-luminescent enzyme activity by comparing the detected luminescence to the luminescence of a control reaction mixture not containing the test compound, or to the reaction mixture containing the test compound at a different concentration.