Patent ID: 7024316
Filing Date: 2006-04-04
Classification: G01N

Abstract:
1. A method of flow cytometry, comprising the steps of: a. establishing a fluid stream; b. entraining particles in said fluid stream; c. perturbing said fluid stream; d. sensing a first occurrence incident to at least one particle; e. generating a first signal from said first occurrence incident to said at least one particle; f. producing data from said first signal; g. sensing at least one additional occurrence incident to said at least one particle; h. generating at least one additional signal from said at least one additional occurrence incident to said at least one particle; i. producing data from said at least one additional signal; j. processing data from said first signal; k processing data from said at least one additional signal; l. applying at least one transformation operation to processed data from said first signal; m. applying at least one transformation operation to processed data from said at least one additional signal; n. compensating at least one parameter shared by said first occurrence and said at least one additional occurrence; o. differentiating said first occurrence from said at least one additional occurrence based upon said at least one compensated parameter; p. assigning said at least one particle to a class; q. separating assigned particles; and r. collecting said assigned particles by class.