Patent ID: 6469779
Filing Date: 2002-10-22
Classification: G01N,G02B

Abstract:
A laser capture microdissection method, comprising:providing a sample that is to undergo laser capture microdissection; positioning said sample within an optical axis of a laser capture microdissection instrument; providing a transfer film carrier having a substrate surface and a laser capture microdissection transfer film coupled to said substrate surface; placing said laser capture microdissection transfer film carrier in juxtaposition with said sample with a pressure sufficient to allow laser capture microdissection transfer of a portion of said sample to said laser capture microdissection transfer film, without forcing nonspecific transfer of a remainder of said sample to said laser capture microdissection film; then transferring a portion of said sample to said laser capture microdissection transfer film, without forcing nonspecific transfer of a remainder of said sample to said laser capture microdissection transfer film; and then removing said laser capture microdissection film from said sample, wherein removing includes moving both said laser capture microdissection transfer film and said portion of said sample away from said remainder of said sample at a velocity to shear said portion of said sample from said remainder of said sample, wherein moving includes raising both said laser capture microdissection transfer film and said portion of said sample up away from said remainder of said sample with a vertical motion.