Patent ID: 6783940
Filing Date: 2004-08-31
Classification: C12Q

Abstract:
A method for reducing non-specific amplification of DNA in a polymerase chain reaction comprising the steps of:(a) providing a sample comprising a target DNA sequence of interest; (b) contacting said sample with at least one enzyme having nucleic acid polymerase activity; and (c) incubating said sample with said enzyme for a time and under conditions sufficient to amplify said target DNA sequence, forming amplified target DNA sequence; wherein said incubation is performed in the presence of an amount of sorbitol and an amount of DMSO effective to reduce said non-specific amplification relative to the amount of non-specific amplification observed in the absence of sorbitol and DMSO, wherein said non-specific amplification is amplification of a region of DNA that is unrelated to said target sequence, amplification of a related but different DNA sequence than the target DNA sequence, or amplification of the target sequence with inexact annealing of at least one primer to said target DNA sequence.