Patent ID: 7402437
Filing Date: 2008-07-22
Classification: G01N,G01R,Y10T

Abstract:
1. A method for preparing a sample and for analyzing the chemical state of living cells by nuclear magnetic resonance (NMR) comprising: i) preparing a sample of living cells so as to set the chemical state of the cells by: ii) storing said sample at said temperature less than or equal to −80° C. iii) just before performing the NMR analysis, mixing and redissolving said lyophilized sample, at a temperature between 0° C. and −20° C., with a solvent having a very low melting point and being liquid at the temperature between 0° C. and −20° C.; iv) obtaining, at the temperature between 0° C. and −20° C., at least one NMR spectrum and/or at least one NMR measurement value through High Resolution Magic Angle Spinninig (HRMAS) NMR; and v) comparing said at least one HRMAS NMR spectrum and/or at least one HRMAS NMR measurement value with at least one reference NMR spectrum and/or at least one reference NMR measurement value obtained on at least one reference sample of living cells.