Patent ID: 6022735
Filing Date: 2000-02-08
Classification: A61K,A61P,C07K,C12N,Y10T

Abstract:
A composition for the transfection of a high eucaryotic cell which comprises a non-naturally occurring nucleic acid complex, wherein said nucleic acid complex comprises(a) a nucleic acid of interest;(b) a substance having affinity for nucleic acid,wherein said substance is optionally coupled with an internalizing factor for said higher eucaryotic cell; and(c) an endosomolytic viral peptide which has the ability of being internalized into said higher eucaryotic cell, either per se or as a component of the nucleic acid complex,wherein the endosomolytic viral peptide has the ability to release contents of the endosome, in which the nucleic acid complex is located after entering the higher eucaryotic cell, into the cytoplasm of said higher eucaryotic cell; and wherein said complex is free of intact infectious virus and free of virus which naturally contain genetic material encoding said endosomolytic viral peptide.