Patent ID: 6472185
Filing Date: 2002-10-29
Classification: C12Q

Abstract:
A composition comprising at least two marker nucleic acids, which specifically hybridize to a nucleotide polymorphism and uniquely maps to a single site in a haploid genomic DNA of a plant or animal, and an amplified mixture of DNA isolated from a biological source, wherein the amplified mixture comprises a heterogeneous amplified mixture comprising a plurality of target nucleic acids comprising polymorphisms in amplified form and wherein the amplified mixture of DNA is made by cleaving a biological genomic DNA sample with at least one restriction enzyme, thereby providing DNA restriction fragments;ligating an adaptor nucleic acid to the DNA restriction fragments; hybridizing primers comprising a subsequence complementary to the adaptor nucleic acid; and, extending the primers with a thermostable DNA polymerase, thereby providing the amplified mixture of DNA comprising a selectively amplified mixture of DNAs.