Patent ID: 6103523
Filing Date: 2000-08-15
Classification: C12N

Abstract:
Pluripotent rabbit cell line, comprising at least 70% undifferentiated, pluripotent cells obtainable by isolating an inner cell mass of a 5.5 day postcoitus blastocyst and culturing the inner cell mass cells on feeder cells in rabbit ES medium,wherein the rabbit ES medium comprises high glucose Dulbecco's Modified Eagle Medium, 4 mM L-glutamine, 0.1 mM 2-mercaptoethanol, 148 units/ml penicillin G sodium, 148 microgram/ml streptomycin sulfate, 4 microgram/ml bovine insulin, 10.sup.3 units/ml murine Leukemia Inhibitory Factor, 20% fetal bovine serum and 1.5% MEM non-essential amino acid solution,wherin the fedder cells are 12.5 days old Mouse Embryonic Feeder cells in a density of 3 to 4.times.10.sup.6 cells per 10 cm petri dish, andwherein the inner cell mass cells are cultured over various passages and the inner cell mass cells are trypsinized before each passage with a selective trypsinization medium consisting of 0.1% collagenase, 1% chicken serum and 0.03% trypsin-ETDA in phosphate buffered saline, such that a pluripotent rabbit cell line results.