Patent ID: 6703240
Filing Date: 2004-03-09
Classification: C08L

Abstract:
A method of making a novel starch metabolizing enzyme (NSME) with a modified substrate usage compared to a parental starch metabolizing enzyme, the method comprising:(i) recombining a plurality of polynucleotide fragments in vitro to produce a library of recombinant polynucleotides, the polynucleotide fragments comprising at least one polynucleotide encoding a parental starch metabolizing enzyme selected from the group consisting of a starch synthase and a branching enzyme; (ii) optionally repeating step (i) one or more times; (iii) introducing the library of recombinant polynucleotides into a plurality of host cells, thereby producing a library of host cells comprising recombinant polynucleotides; and (iv) identifying at least one host cell with modified substrate usage confined by a recombinant polynucleotide encoding an NSME compared to a host cell comprising a polynucleotide encoding the parental starch metabolizing enzyme, thereby identifying at least one recombinant polynucleotide encoding an NSME with a modified substrate usage.