Patent ID: 6369048
Filing Date: 2002-04-09
Classification: A61K,A61L,Y02A

Abstract:
A method of inactivating a non-enveloped virus in a biological composition, said method comprising the steps of:(a) contacting the composition with an organic solvent under viral inactivating conditions, wherein said organic solvent is selected from the group consisting of (i) trialkylphosphates of the formula PO(OR1)(OR2)(OR3), wherein each of R1, R2, and R3 is, independently, C1-10 alkyl, (ii) ethers of the formula R4â€”Oâ€”R5, wherein each of R4 and R5 is, independently, a C1 to C18 alkyl or alkenyl radical which can contain an oxygen or sulfur atom, and (iii) alcohols of the formula R6â€”OH, wherein R6 is a C1 to C18 alkyl or alkenyl radical which can contain 1 to 4 oxygen or sulfur atoms, inclusive, in the chain, and which can be substituted by 1 to 4 hydroxy groups, inclusive; (b) contacting the composition with an ethyleneimine oligomer under viral inactivating conditions; and (c) contacting the composition with a nonionic detergent under viral inactivating conditions, wherein steps (a), (b), and (c) are performed substantially simultaneously.