Patent ID: 8213004
Filing Date: 2012-07-03
Classification: G01J,G01N

Abstract:
1. A method of measuring Raman signals, comprising: an analyte placing step of placing an analyte on a detection surface of a microstructure plate which generates an enhanced electric field when irradiated with excitation light; an irradiating step of irradiating the detection surface with the excitation light so that the enhanced electric field is generated around the detection surface and Raman scattered light is emitted from the analyte and the detection surface to be enhanced by the generated enhanced electric field; a Raman signal obtaining step of detecting the enhanced Raman scattered light emitted from the analyte to obtain a Raman signal from the analyte having a wavelength and detecting the enhanced Raman scattered light emitted from the detection surface of the microstructure plate to obtain at least one background signal for use as a reference having at least one specific wavelength which differs from the wavelength of the Raman signal from the analyte; an intensity measuring step of measuring respective intensities of the Raman signal from the analyte and the at least one background signal from the detection surface of the microstructure plate; and a normalizing step of normalizing the Raman signal from the analyte by dividing the measured intensity of the Raman signal from the analyte by the measured intensity of the at least one background signal from the detection surface of the microstructure plate obtained as the reference.