Patent ID: 7283245
Filing Date: 2007-10-16
Classification: G01N

Abstract:
1. A method for measuring analyte concentration of a chemical or a biological substance, said method comprising the steps of: a. providing a reagent film with at least one internal reference standard; b. immobilizing a layer of said film onto a test element, thereby providing a film coated test element; c. emitting light energy onto a coated test element, wherein said light energy undergoes internal reflection and multiangle scattering inside said test element, said light energy being effective to stimulate a dual reference light response from said coated test element; d. exposing said coated test element to a sample substance for a specified time period, then removing said exposed test element from said substance, thereby providing a sample test element; e. emitting light energy onto said sample test element, said light energy being effective to stimulate a dual sample light response from said sample test element; f. collecting and processing said reference and sample light response data to calculate a light absorption response; g. utilizing said light absorption response to detect and quantify analyte concentration in said substance; and h. generating a signal indicative of said analyte concentration based on said detection and quantification.