Patent ID: 6689571
Filing Date: 2004-02-10
Classification: G01N

Abstract:
A method for assaying for Pythium insidiosum antibodies in a serum sample which comprises:(a) providing: (1) isolated Pythium insidiosum antigens comprising dominant 30-33 Kd extracellular proteins which complex with Pythium insidiosum antibodies that can be present in a serum sample; (2) particles which complex with the Pythium insidiosum antigens; (3) a negative control serum which is free of Pythium insidiosum antibodies; (4) a positive control serum which contains Pythium insidiosum antibodies; and (5) at least one device for separately mixing the antigen and the particles with (1) the serum sample; (2) the positive control serum, and (3) the negative control serum, wherein in the assay if the serum sample contains the Pythium insidiosum antibodies which complex with the antigen, the particles are agglutinated and are compared to the positive and negative control serums; and (b) testing the serum sample along with negative and positive controls with the particles and the antigens to determine if there is agglutination of the particles by the sample serum.