Patent ID: 6361938
Filing Date: 2002-03-26
Classification: A61K,A61P,B82Y,C12N,C40B,G01N,Y10S

Abstract:
A method of identifying a peptide which permits or facilitates the transport of an active agent through a human or animal tissue, comprising the steps of:(a) administering in vivo or in situ to a site in an animal a predetermined amount of phage from a random phage library or a preselected phage library; (b) at a predetermined time harvesting phage from the animal to select transported phage, wherein the harvesting site is separated from the site of administration by a tissue barrier; (c) amplifying the transported phage in a host; (d) repeating in order step (a) using the transported phage obtained in step (b) and amplified in step (c) and steps (b) and (c) a predetermined number of times to obtain a selected phage library containing phage which can be transported from the site of administration to the site of harvesting; and (e) determining the identity of at least one peptide coded by phage in the selected phage library to identify a peptide which permits or facilitates the transport of an active agent through a human or animal tissue.