Patent ID: 6468757
Filing Date: 2002-10-22
Classification: G01N,Y02E

Abstract:
A method of screening for drug binding to serum proteins, said method comprising:preparing at least two solutions, each of said at least two solutions comprising a concentration of a gerum protein characterized by broad specificity in binding to xenobiotics and a concentration of a candidate drug, wherein the concentration of the candidate drug is different for each of the at least two solutions and, optionally, one of the at least two solutions is a control solution characterized by a candidate drug concentration of zero; exposing each of the at least two solutions to a light source; measuring fluorescent emission by the serum protein or a serum protein-candidate drug complex for each of the at least two solutions upon said exposing; and determining whether a change in fluorescence emission is measured for an increased concentration of the candidate drug, wherein the change in fluorescence emission indicates binding of the candidate drug to the serum protein.