Patent ID: 7767647
Filing Date: 2010-08-03
Classification: A61K,A61P

Abstract:
1. A method of making a preparation of Factor IXa, wherein the preparation contains little or no impurities having prekallikrein activator activity, said method comprising; a) dissolving a paste of Cohn fraction IV- 1; b) adsorbing clotting factors contained in said Cohn fraction IV-1 onto calcium phosphate; c) eluting said clotting factors from said calcium phosphate to form a first eluate; d) applying said first eluate to an anion exchange resin, thereby adsorbing Factor IXa to said resin and allowing impurities having prekallikrein activator activity to flow through into a discard fraction; and e) eluting and collecting said Factor IXa from said resin.