Patent ID: 6187761
Filing Date: 2001-02-13
Classification: A23K,A23L,A23V,A61K,A61Q,C12P,Y10S

Abstract:
A composition comprising natural vitamin B12 in an amount ranging from about 0.1% by weight to about 10% by weight based on the dry matter content of said composition, wherein said composition is obtained by a process comprising:(a) culturing microbial cells capable of producing natural vitamin B12 under conditions such that said cells produce natural vitamin B12 intracellularly;(b) disrupting the outer membrane of said cells such that said natural vitamin B12 is released from said cells into the medium in which the cells are cultured to produce a liquid phase containing the natural vitamin B12 and a solid phase;(c) separating the liquid phase from the solid phase to obtain a preparation containing natural vitamin B12; and(d) recombining portion of the solid phase with the liquid phase, the solid phase comprising at least a part of the disrupted cells.