Patent ID: 6087184
Filing Date: 2000-07-11
Classification: G01N,Y10S,Y10T

Abstract:
A chromatographic assay device for detection or determination of an analyte in a test sample comprising:(a) a first opposable opponent including:(i) a first chromatographic medium having first and second ends and an immobilized first specific binding partner for the analyte in a detection zone;(ii) a conjugate pad in operable contact with the first end of the first chromatographic medium so that fluid applied to the conjugate pad flows from the conjugate pad to the first end of the first chromatographic medium, the conjugate pad including:(A) a first portion containing a labeled second specific binding partner for the analyte in resolubilizable form;(B) a second portion; and(C) a third portion containing a labeled second specific binding partner for the analyte in resolubilizable form, the second portion separating the first portion and the third portion; and(iii) a second chromatographic medium having first and second ends and having immobilized thereon in a reference zone a third specific binding partner, other than the analyte or an analyte analogue, that specifically binds the labeled second specific binding partner for the analyte and does not bind the analyte, the first end of the second chromatographic medium being in operable contact with the conjugate pad so that fluid applied to the conjugate pad flows from the conjugate pad to the first end of the second chromatographic medium; and(b) a second opposable component including a sample application zone; wherein the first and second opposable components are brought into opposition to apply a sample applied to the sample application zone to the conjugate pad to resolubilize the labeled second specific binding partner so that the sample and the resolubilized labeled second specific binding partner in the first portion of the conjugate pad are applied to the first chromatographic medium and the sample and the resolubilized labeled second specific binding partner in the third portion of the conjugate pad are applied to the second chromatographic medium for detection of the analyte.