Patent ID: 6642037
Filing Date: 2003-11-04
Classification: A61L,A62D,C08G,C12N

Abstract:
A method of making an enzymatically active reusable polymeric sponge or foam, capable of regeneration with oximes, for the detoxification of a hazardous compound comprising immobilizing a plurality of enzymes on or within the sponge or foam by mixing the plurality of enzymes with a polyurethane prepolymer in an apparatus having a static mixing stator connected to both first chamber and a second chamber, wherein the first chamber contains a mixture of the plurality of enzymes and the second chamber contains the polyurethane prepolymer, and equal parts of the mixture of the plurality of enzymes and the polyurethane prepolymer are passed from the first and second chambers into the static mixing stator where the enzymes and prepolymer are mixed under low shear conditions while being rapidly and evenly extruded through the static mixing stator to form said sponge or foam, said plurality of enzymes capable of detoxifying organophosphorous and/or organosulfur compounds, wherein said plurality of enzymes comprises at least one enzyme selected from the group consisting of: acetylcholinesterase (AchE), butylcholinesterase (BchE), triesterase, pseudocholinesterase, organophosphate hydrase (OPH), phosphotriesterase, paraoxonase and organophosphorus and organosulfur (OP) hydrolyzing enzymes.