Patent ID: 7985552
Filing Date: 2011-07-26
Classification: C07D,C09B,G01N,Y10T

Abstract:
1. A method of analyzing a biological sample, wherein the method comprises combining a dye solution comprising a conjugate of a biological substance prepared from dye I, wherein C is a non-conjugated chain of 10 to 50 linear atoms selected from a group consisting of carbon and nitrogen that are further substituted by substituents selected from a group consisting of hydrogen, alkyl having 1-20 carbons, hydroxyl, carbonyl, and RGM; n is 0, 1, 2 or 3; R R RGM is a carboxylic acid, an activated ester of a carboxylic acid, an acyl azide, an acyl nitrile, an imido ester, an isocyanate, an isothiocyanate, or a maleimide; wherein the biological substance is selected from a group consisting of a peptide, protein, nucleotide, nucleic acid, carbohydrate, lipid, membrane or cell, and wherein the conjugate is analyzed by detecting optical response upon use of an illumination source.