Patent ID: 6933128
Filing Date: 2005-08-23
Classification: C12Q,G01N

Abstract:
1. A method for screening a test substance for COX-2 inhibitory activity comprising: (a) contacting the test substance with indicator cells which express a GTPase-deficient mutant form of the α-subunit of protein G12, wherein the G12 protein α-subunit mutant comprises the Q229L mutation, which mutant α-subunit has the capacity to induce the production of arachidonic acid in the indicator cells, and induces production of COX-2 in the indicator cells; and (b) determining the level of one or more prostaglandins produced by the indicator cells in the presence and absence of the test substance, a decrease in production of said prostaglandin by the indicator cells in the presence of the test substance indicating that the test substance has COX-2 inhibitory activity.