Patent ID: 6617492
Filing Date: 2003-09-09
Classification: A01H,C12Q,Y02A

Abstract:
A method of screening a plant to determine whether the plant is a Tripsacum daclyloides/Zea diploperennis hybrid, said method comprising:isolating the total genomic DNA from said plant; digesting said genomic DNA with one to four of the restriction enzymes selected from the group consisting of EcoRI, EcoRV, HindIII and BamHI; probing said digested genomic DNA with one or more DNA markers selected from the group consisting of the maize nuclear DNA probes, maize mitochondrial DNA probes, and Tripsacum DNA probes recited below; and determining the presence of one or more of the following restriction fragments of the following fragment sizes, wherein said restriction fragments are characterized by the following molecular marker-restriction enzyme associations and the associated fragment sizes selected from the group consisting of: BNL5.62, EcoRI, 10.3 kb; npi97, HindIII, 3.9 kb; UMC157, EcoRI, 6.5 kb and 3.3 kb; UMC157, HindIII, 5.5 kb; UMC157 BamHI, 14.0 kb, 8.6 kb and 4.5 kb; UMC11, BamHI, 7.0 kb; CSU3, BamHI, 10.0 kb, 7.6 kb and 3.5 kb; UMC67, EcoRI, 19.2 kb; UMC67, BamHI, 13.4 kb and 1.6 kb; CSU92, BamHI, 13.3 kb and 7.5 kb; 62 BamHI, 12.7 kb, 9.7 kb and 6.6 kb; UMC58, HindIII, 3.3 kb; CSU164, EcoRI, 9.0 kb and 7.0 kb; UMC128, HindIII, 6.0 kb; UMC107, EcoRI, 6.3 kb and 5.3 kb; UMC107, HindIII, 19.2 kb; UMC140, EcoRI, 5.0 kb; UMC140, HindIII, 6.5 kb; UMC107, EcoRI, 6.3 kb; adh1, HindIII, 9.4 kb; adh1, BamHI, 9.4 kb; UMC161, HindIII, 3.3 kb; BNL8.29, HindIII, 8.3 kb; UMC53, EcoRI, 9.4 kb, 3.8 kb and 3.0 kb; UMC53, EcoRV, 8.4 kb, 3.8 kb and 3.0 kb; UMC6, EcoRI, 3.8 kb; UMC6, HindIII, 9.4 kb; UMC6, BamHI, 13.2 kb, 12.7 kb, and 7.0 kb; UMC61, HindIII, 3.4 and 2.8 kb; UMC34, EcoRI, 7.5 kb and 5.4 kb; UMC34, HindIII, 8.8 kb, 6.5 kb and 5.8 kb; UMC135, HindIII, 11.6 kb and 10.8 kb; UMC131, EcoRI, 10.6 kb, 5.8 kb and 4.3 kb; UMC55, EcoRI, 3.9 kb; UMC55, HindIII, 4.3 kb; UMC5, EcoRI, 5.4 kb; UMC5, HindIII, 6.5 kb; UMC49, BamHI, 8.2kb, 6.0 kb, 4.2 kb, and 3.2 kb; UMC36, BamIII, 4,2 kb; UMC32, HindIII, 6.7 kb; asg24, HindIII, 7.2 kb and 6.4 kb; UMC121, EcoRI, 3.7 kb and 3.2 kb; BNL8.35, HindIII, 9.9 kb and 8.7 kb; UMC50, BamHI, 7.8 kb, 6.8 kb, 5.8 kb and 3.8 kb; UMC42, HindIII, 10.4 kb, 8.9 kb, 7.6 kb, 3.7 kb and 3.0 kb; npi247, EcoRI, 8.0 kb; npi247, HindIII, 3.0 kb; UMC10, HindIII, 5.9 kb and 3.0 kb; UMC10, EcoRI, 6.5 kb and 5.5 kb; UMC102, EcoRI, 2.7 kb; BNL6.06, EcoRI, 6.8 kb; BNL5.37, HindIII, 10.3 kb, 5.8 kb and 3.5 kb; npi296, EcoRI, 7.9 kb; UMC3, EcoRI, 2.5 kb and 2.0 kb; npi212, HindIII, 4.3 kb; npi212, BamHI, 5.4 kb; UMC39, EcoRI, 12.2 kb, 9.2kb, 7.8 kb and 7.1 kb UMC63, HindIII, 9.5 kb and 4.3 kb; UMC96, HindIII, 11.8 kb, 6.4 kb and 5.5 kb; UMC96, BamHI, 7.5 kb; UMC2, EcoRI, 11.8 kb, 10.4 kb and 8.0 kb; CSU25, HindIII, 4.5 and 4.3 kb; agrr115, EcoRI, 8.0 kb and 5.4 kb; agrr115, BamHI, 5.4 kb and 3.5 kb, phi20725, EcoRI, 10.3 kb, 9.7 kb and 7.2 kb; phi20725, HindIII, 1.5 kb; UMC31, EcoRI, 5.8 kb; UMC31, BamHI 6.5 kb; BNL5.46, HindIII, 13.7 kb, 10.5 kb, 9.7 kb and 5.1 kb; npi386, HindIII, 12.6 kb, 9.3 kb and 8.2 kb; tda62, BamHI, 5.5 kb and 4.8 kb; BNL5.71, EcoRI, 11.3 kb, 6.8 kb, and 5.7 kb; BNL5.71, BamHI, 11.3 kb, 6.8 kb and 5.7 kb; UMC156, HindIII, 3.0 kb; UMC66, EcoRI, 10.5 kb and 6.5 kb; UMC66, BamHI, 3.7 kb; UMC19, BamHI, 12.3 kb; UMC104, HindIII, 12.4 kb, 11.6 kb and 7.5 kb; UMC104, BamHI, 9.4 kb; UMC133, HindIII, 10.6 kb, 9.9 kb, 9.2 kb and 7.7 kb; UMC52, BamHI, 8.7 kb, 6.9 kb, 3.8 kb, 3.0 kb and 2.0 kb; BNL15.07, HindIII, 2.9 kb and 2.7 kb; npi409, EcoRI, 9.4 kb; npi409, HindIII, 10.4 kb, 9.0 kb and 3.9 kb; UMC147, HindIII, 16.3 kb, 3.8 kb and 2.4 kb; UMC90, HindIII, 7.8 kb, 2.8 kb and 2.5 kb; UMC90, BamHI, 9.0 kb; UMC27, HindIII, 8.3 and 4.5 kb; tda37, BamHI, 8.2 kb and 6.5 kb; UMC43, BamHI, 9.7 kb, 7.3 kb and 5.7 kb; UMC40, BamHI, 7.2 kb, 4.3 kb and 4.0; BNL7.71, HindIII, 10.6 kb; tda62, BamIII, 5.5 kb; UMC68, HindIII, 6.0 kb; phi10017, BamHI, 15.1 kb and 9.5 kb; tda50, BamHI, 8.5 kb; npi373, HindIII, 6.5 kb, 5.6 kb and 3.0 kb; tda204, BamHI, 4.0 kb; npi393, EcoRI, 12.1 kb, 8.5 kb, 7,0 kb and 5.6 kb; UMC65, HindIII, 2.9 kb; UMC46, EcoRI, 6.5 kb and 5.6 kb; asg7, HindIII, 6.3 kb; UMC28, HindIII, 15.8 kb and 11.9 kb; UMC28, BamHI, 10.0 kb, 7.6 kb and 6.6 kb; UMC134, BamHI, 4.7 kb; UMC134, HindIII, 7.5 kb; asg8, HindIII, 10.8 kb, 8,7 kb and 8.4 kb; O2, EcoRI, 9.4 kb; BNL15.40, HindIII, 5.8 kb; UMC116, EcoRI, 9.5 kb; UMC110, BamHI, 10.6 kb, 4.9 kb and 3.9 kb; BNL8.32, HindIII, 8.9 kb, 7.4 kb and 7.1 kb; BNL14.07, EcoRI, 6.4 kb, UMC80, HindIII, 10.6 kb, 8.2 kb and 2.4 kb; BNL16.06, EcoRI, 6.8 kb and 1.9 kb; phi20020, HindIII, 7.8 kb and 6.6 kb; npi114, HindIII, 10.0 kb, 8.8 kb and 6.3 kb; BNL9.11, HindIII, 3.4 kb; UMC103, HindIII, 6.9 kb and 5.7 kb; UMC124, HindIII, 8.0 and 7.0; UMC124, BamHI, 6.6 kb and 2.6 kb; UMC120, HindIII, 3.2 kb, 2.3 kb and 1.4 kb; UMC89, EcoRI, 7.3 kb; UMC89, HindIII, 7.3 kb; UMC89, BamHI, 9.5 kb, 6.0 kb, 5.2 kb and 4.5 kb; BNL12.30, EcoRI, 3.5 kb; UMC48, HindIII, 6.2 kb, 5.3 kb, 4.7 kb, 3.5 kb and 2.2 kb; npi268, BamHI, 6.4 kb; phi10005, EcoRI, 15.0 kb; UMC113, EcoRI, 5.9 kb and 5.4 kb; UMC113, BamHI, 12.8 kb, 11.8 kb and 10.5 kb; UMC192, HindIII, 11.4 kb and 6.4 kb; wx(waxy), HindIII, 21.0 kb; CSU147, HindIII, 5.9 kb; BNL5.10, HindIII, 6.1 kb and 4.4 kb; UMC114, BamHI, 12.6 kb, 11.5 kb, 10.0 kb, 8.8 kb, 7.5 kb and 6.5 kb; UMC95, EcoRI, 5.6 kb; UMC95, HindIII, 7.7 kb, 4.8 kb and 4.1 kb; UMC95, BamHI, 15.0 kb and 9.0 kb; CSU61, EcoRI, 8.1 kb and 4.8 kb; BNL7.57, EcoRI, 1.0 kb; BNL7.57, BamHI, 11.6 kb, 5.9 kb and 1.3 kb; CSU54, EcoRI, 14.7 kb and 12.6 kb; phi20075, EcoRI, 7.1 kb; npi285, EcoRI, 12.4 kb, 9.4 kb and 6.0 kb; KSU5, EcoRI, 9.8 kb, 7.6 kb, 6.1 kb, 3.8 kb and 3.5 kb; UMC130, EcoRI, 13.5 kb and 7.0 kb; UMC130, HindIII, 4.8 kb and 3.2 kb; UMC130, BamIII, 3.2 kb; UMC152, HindIII, 12.4, 7.1 kb and 5.6 kb; UMC64, HindIII, 3.3 kb; phi06005, EcoRI, 12.8 kb; UMC163, HindIII, 7.0 kb, 4.8 kb and 2.6 kb; UMC44, HindIII, 9.8 kb, 8.7 kb, 7.2 kb, 5.5 kb and 4.0 kb; BNL10.13, HindIII, 10.8 kb; npi306, HindIII, 7.0 kb; pmt1, HindIII, 2.3 kb; pmt2, HindIII, 8.0 kb, 4.2 kb, 2.8 kb and 2.1 kb; pmt5, HindIII, 12.3 kb, 8.1 kb, 3.6 kb, 3.2 kb and 2.5 kb; tda48, HindIII, 8.2 kb; tda53, HindIII, 3.8 kb and 2.2 kb; tda168, EcoRI, 3.6 kb; tda16, HindIII, 4.3 kb; and tda17, HindIII, 7.0 kb.