Patent ID: 8598319
Filing Date: 2013-12-03
Classification: A61K,A61P,C07K,C12N

Abstract:
1. A process for separating proteins fibrinogen, Factor XIII (FXIII) and biological glue from a solubilized plasma fraction, containing fibrinogen and Factor XIII, and for preparing freeze-dried concentrates of said proteins comprising the steps of: a) chromatographic purification comprising the steps of: b) separating FXIII from fibrinogen by addition to at least a part of the biological glue eluate of at least one chemical agent precipitating the FXIII, wherein the chemical agent precipitating the FXIII is present in the form of an aqueous solution comprising 1M sodium citrate, recovering the resulting supernatant solution of purified fibrinogen, and resolubilizing the precipitated FXIII, and c) diafiltering the fibrinogen, biological glue and resolubilized FXIII solutions, followed by freeze-drying of said solutions.