Patent ID: 7906314
Filing Date: 2011-03-15
Classification: C07H,C12N,C12P

Abstract:
1. A method for removal of monosaccharide in an oligosaccharide mixture, the method comprising: providing an oligosaccharide mixture containing about 75% non-digestable and non-fermentable isomaltooligosaccharides (W/W), with a balance of the oligosaccharide mixture containing monosaccharides; mixing the oligosaccharide mixture with 8%-12% yeast (W/W) based on the weight of the oligosaccharide mixture and 0.1%-0.5% (W/W) of carbamide as a nitrogen source; adjusting pH to 4.5-6.0; and fermenting the oligosaccharide mixture for 20-30 hours at a temperature of 23° C. to 26° C. while agitating the oligosaccharide mixture intermittently to remove the monosaccharides and increase the percentage of non-digestable and non-fermentable isomaltooligosaccharides in the oligosaccharide mixture.