Patent ID: 6780620
Filing Date: 2004-08-24
Classification: C12P

Abstract:
A method for the preparation of at least one hydroxyalkyl-bearing compound of the following formula III: whereQ is selected from the group consisting of G1 is the following formula V HOâ€”CH2â€”(A1)nâ€”(Q1)mâ€”(A2)oâ€ƒâ€ƒ(V), whereA1 and A2 are independently selected from the group of optionally substituted C1-C3 alkyl and alkenyl; Q1 is an optionally substituted ring system containing one to three rings and at least one carbon to carbon double bond in at least one ring; n, m, and o are integers independently selected from the group consisting of zero and 1, where at least one of m or n or o is 1; W is O or NR6; X is selected from the group consisting of O and H, OR7; M is O, S, NR8, or CR9R10; B1 and B2 are selected from the group consisting of OR11, and OCOR12; R1-R5 and R12-R17are selected from the group consisting of H, alkyl, substituted alkyl, aryl, and heterocyclo, and wherein when R1 and R2 are alkyl they can be joined to form a cycloalkyl; R6 is selected from the group consisting of H, alkyl, and substituted alkyl; R7 and R11 are selected from the group consisting of H, alkyl, substituted alkyl, trialkylsily, alkyldiarylsilyl, and dialkylarylsilyl; R8 is selected from the group consisting of H, alkyl, substituted alkyl, R13C&boxH;O, R14OC&boxH;O and R15SO2; R9 and R10 are selected from the group consisting of H, halogen, alkyl, substituted alkyl, aryl, heterocyclo, hydroxy, R16C&boxH;O, and R17OC&boxH;O; the pharmaceutically acceptable salts thereof and any hydrates, solvates, or geometric, optical and stereoisomers thereof; comprising the steps of contacting at least one compound of the following formula IV: whereQ, W, X, M, B1, B2, and R1-R17 are as defined above; G2 is the following formula VI CH3â€”(A1)nâ€”(Q1)mâ€”(A2)oâ€ƒâ€ƒ(VI), where A1, Q1, A2, n, m, and o are defined as above; the pharmaceutically acceptable salts thereof and any hydrates,solvates, or geometric, optical and stereoisomers thereof; with a microorganism, or P-450 enzyme isolated therefrom, said microorganism or P-450 enzyme capable of selectively catalyzing the hydroxylation of G2 to G1 and effecting said hydroxylation.