Patent ID: 8105845
Filing Date: 2012-01-31
Classification: B01J,B01L,C40B,G01N

Abstract:
1. A method for determining one or more kinetic parameters of binding between a first binding member and a second binding member comprising: simultaneously adsorbing the first binding member to a surface at a plurality of microspots, the adsorbing comprising simultaneously presenting the second binding member at a plurality of concentrations to the first binding member at the plurality of microspots, there being a plurality of combinations of first binding member surface density and second binding member concentrations among the plurality of microspots; simultaneously obtaining one or more kinetic parameters indicative of a binding reaction between the first and second binding members at each of the plurality of microspots to produce a kinetic analysis of the binding, the binding being detected by a biosensor detection method; simultaneously obtaining reference data from a plurality of interspots, each of the interspots located at a surface between at least two or more the microspots; and processing the binding kinetic parameters and the reference data to obtain one or more kinetic parameters characteristic of the binding between the first and second binding members, wherein the plurality of bindings carried out does not require a regeneration step.