Patent ID: 6746844
Filing Date: 2004-06-08
Classification: C12N

Abstract:
A method of determining candidate functional mediators of a selected gene, comprising the steps of:a. altering expression of a first selected gene in a first of two populations of identical cells; b. comparing expression levels of a first set of genes in the two populations of cells, wherein the expression levels are measured on oligonucleotide arrays; c. identifying genes in the first set whose expression levels differ between the two populations of cells, wherein the genes identified are candidate functional mediators of the selected gene; d. altering expression of a second selected gene in one of a third and fourth populations of cells, wherein the third and fourth populations comprise identical cells, wherein the second selected gene is a candidate functional mediator of the first selected gene; e. comparing expression levels of a second set of genes in the third and fourth populations of cells, wherein the expression levels are measured on oligonucleotide arrays; f. identifying genes in the second set whose expression levels differ between the third and fourth populations of cells, wherein the genes identified are candidate functional mediators of the second selected gene.