Patent ID: 6919183
Filing Date: 2005-07-19
Classification: A01K,C07K,C12N,C40B,G01N

Abstract:
1. A method of detecting and/or isolating a eukaryotic cell that produces a secreted protein of interest (POI), comprising: (a) providing a cell comprising a nucleic acid that encodes a secreted POI and a nucleic acid that encodes a cell surface capture molecule capable of binding the POI, wherein the nucleic acid encoding the secreted POI or the nucleic acid encoding the cell surface capture molecule, or both, are transfected into the cell; (b) culturing the cell under conditions in which the POI and cell surface capture molecule are expressed, and a POI-capture molecule complex is formed intracellularly and displayed on the cell surface; (c) contacting the cell with a detection molecule, which binds to the POI displayed by the cell; and (d) detecting and/or isolating the cell due to it being bound to the detection molecule.