Patent ID: 8624020
Filing Date: 2014-01-07
Classification: C12N

Abstract:
1. A method for the washing of nucleic acids immobilized on one or more surfaces, wherein the nucleic acids are brought into contact with a wash buffer comprising less than 24% by volume of alcohol having 1 to 3 carbon atoms and at least one further solvent selected from the group consisting of alkane diols and alkane triols having 2 to 6 carbon atoms, monocarboxylic acid esters and dicarboxylic acid diesters having 2 to 6 carbon atoms in the acidic component and 1 to 4 carbon atoms in the alcoholic component; (poly)ethylene glycols and ether derivatives and ester derivatives thereof, with “poly” representing 2 to 200 repetitive ethylene glycol units, poly-(2-ethyl-2-oxazoline); and poly(4-styrene sulfonic acid-co-maleic acid) sodium salt solution.