Patent ID: 7435555
Filing Date: 2008-10-14
Classification: C07K,C12Q,G01N

Abstract:
1. A method of screening for agonists or antagonists of an asparagine hydroxylase capable of hydroxylating a target asparagine, the asparagine hydroxylase being FIH (Factor Inhibiting Hypoxia Inducible Factor); the method including the steps of: i) contacting with a preparation containing FIH, under conditions suitable for hydroxylation, a peptide or protein comprising the target asparagine and including a hydroxylation motif of amino acids necessary for hydroxylation by the asparagine hydroxylase, said hydroxylation motif being selected from the group consisting of SEQ ID NO: 4 and SEQ ID NO: 5 ii) additionally contacting the mixture with a candidate agonist or antagonist, and iii) measuring directly or indirectly either inhibition or enhancement of hydroxylation of the target asparagine.