Patent ID: 8961954
Filing Date: 2015-02-24
Classification: A61K,A61P,C12N

Abstract:
1. A method of treating a urinary tract disease in a mammalian subject in need thereof comprising: (a) isolating skeletal muscle cells from a human; (b) cooling the skeletal muscle cells to a temperature lower than 10° C. and storing the cells for 1-7 days; (c) suspending the human skeletal muscle cells obtained in step (b) in a first cell culture container for between 30 and 120 minutes to which fibroblast cells of the cells from the skeletal muscle cell suspension adhere, wherein the remaining cells remain non-adherent; (d) decanting the medium from the first cell culture container to re-plate the non-adherent cells from step (c) in a second cell culture container, wherein the step of decanting is after 15-20% of cells have adhered to the first container; (e) allowing the non-adherent cells in the medium in the second cell culture container to attach to the walls of the second cell culture container; (f) repeating step (d)-(e) at least once, wherein the decanting is after 15-20% of cells have adhered to the wall of the previous cell culture container; (g) isolating the cells from the walls of the second cell culture container of step (e) or the container of step (f), wherein the isolated cells are skeletal muscle-derived progenitor cells (MDCs) and are desmin positive; and administering the MDCs to the urinary tract of the mammalian subject; thereby treating urinary tract disease in a mammalian subject in need thereof.