Patent ID: 7790425
Filing Date: 2010-09-07
Classification: C12N,C12P

Abstract:
1. A method for producing soyasapogenol B, which comprises decomposing a glycoside having soyasapogenol B as an aglycone using a culture containing a protein having saponin-decomposing activity, which is obtained by transforming a host with a recombinant vector comprising a polynucleotide encoding a protein having saponin-decomposing activity, wherein said polynucleotide is selected from the group consisting of: (i) a polynucleotide consisting of the DNA sequence of SEQ ID NO: 1; (ii) a polynucleotide that has at least 95% homology to the polynucleotide of (i) and encodes a protein having saponin-decomposing activity, wherein the protein produces soyasapogenol B from saponin in a culture; (iii) a polynucleotide that hybridizes with the polynucleotide of (i) under stringent conditions and encodes a protein having saponin-decomposing activity, wherein said stringent conditions comprise two washes at 42° C. for 20 minutes each in 0.5×SSC, 0.4% SDS and 6M urea, and wherein the protein produces soyasapogenol B from saponin in a culture; (iv) a polynucleotide encoding a protein comprising the amino acid sequence of SEQ ID NO: 2; and (v) a polynucleotide encoding a protein that has at least 95% homology to the amino acid sequence of SEQ ID NO: 2 and having saponin-decomposing activity, wherein the protein produces soyasapogenol B from saponin in a culture.