Patent ID: 9086404
Filing Date: 2015-07-21
Classification: G01N

Abstract:
1. A method for providing a reagent to living cells in a sample comprising the steps of: providing a film having a thickness between 1 μm and 10 μm comprising a porous body and having a top face and opposite bottom face, wherein pores of the porous body are not interconnected to each other and are present independently, wherein the pores have a diameter of 10 nm to 10 μm, and are smaller than a diameter of the cells in the sample, and wherein the pores perforate through the film from the top face to the bottom face such that the pores are vertical or have an angle of 15° or less from the vertical; placing the living cells in the sample on a supporting member; contacting the bottom face of the film directly with the living cells on the supporting member such that each pore of the porous body corresponds to one living cell on the supporting member; and discharging by ink-jet a solution as a droplet containing at least one kind of reagent to the top face of the film not contacting the living cells such that the solution passes through the pores to the bottom face to contact the living cells to supply the reagent to each individual living cell separately.