Patent ID: 6300079
Filing Date: 2001-10-09
Classification: C07K,Y02P,Y10S

Abstract:
A process for measuring an analyte comprising:reacting a sample, of body fluids or cells, containing an analyte with a product which is a polypeptide bound to an affinity substance having an affinity for the analyte, said polypeptide having a total of 3 to 30 amino acid residues with at least three --SO.sub.3 H or --PO.sub.3 H.sub.2 groups introduced into the free reactive groups of the amino acid residues, whereby the reaction forms a resulting complex composed of the analyte and the product,separating the resulting complex by subjecting the resulting complex to an anion exchange method,eluting the separated resulting complex by increasing a concentration of a salt in an eluent, anddetermining the amount of the analyte in the sample on the basis of the amount of the separated resulting complex eluted.