Patent ID: 8900870
Filing Date: 2014-12-02
Classification: A61M,G01N,Y10T

Abstract:
1. A method of forming a hybridoma cell, comprising: acoustically focusing antibody producing cells in a first channel having a first acoustic field so as to produce a single file line of antibody producing cells flowing along the first channel; acoustically focusing immortal cells in a second channel having a second acoustic field so as to produce a single file line of immortal cells flowing along the second channel; flowing the acoustically focused single file line of antibody producing cells and the acoustically focused single file line of immortal cells to a third channel having a third acoustic field configured to bring at least one antibody cell from the acoustically focused single file line of antibody producing cells and at least one immortal cell from the acoustically focused single file line of immortal cells into close enough proximity to permit the at least one antibody producing cell and the at least one immortal cell to fuse; and fusing the at least one antibody producing cell and the at least one immortal cell together by at least one of a chemical and an electrical means to form at least one hybridoma cell.