Patent ID: 8839961
Filing Date: 2014-09-23
Classification: B01D,B01J,B82Y,G01N

Abstract:
1. A method for producing a biosensor, which comprises: (i) active-esterifying a polysaccharide having a carboxyl group to obtain a polysaccharide having a carboxylic acid active ester group; and (ii) bringing into contact, by a spin coating method, the polysaccharide having a carboxylic acid active ester group with a substrate surface coated with an organic layer having a functional group, to thereby bind the polysaccharide having a carboxylic acid active ester group to the organic layer, wherein an aqueous solution, consisting essentially of water, and containing the polysaccharide having a carboxylic acid active ester group is coated on the substrate surface and water of the aqueous solution is evaporated to form a thin film of the polysaccharide having a carboxylic acid active ester group bound to the organic layer coated on the substrate surface, the organic layer having a functional group is a self-assembled monolayer or one or more alkanethiols, the self-assembled monolayer having an amino group, and the polysaccharide having a carboxylic acid active ester group is covalently bound to the organic layer on the substrate surface by a reaction between said amino group and said carboxylic acid active ester group.