Patent ID: 6753421
Filing Date: 2004-06-22
Classification: C07K,C12Q

Abstract:
A peptide nucleic acid probe comprising from 15 to 30 polymerized moieties of formula (I) wherein each X and Y are independently chosen from O and S, each Z independently is chosen from O, S, NR1, and C(R1)2, wherein each R1 is independently chosen from H, C1-6 alkyl, C1-6 alkenyl, and C1-6 alkynyl, each R2, R3 and R4 are independently chosen from H, the side chain of a naturally occurring amino acid, the side chain of a non-naturally occurring amino acid, C1-4 alkyl, C1-4 alkenyl, C1-4 alkynyl, and a functional group, and each Q is independently chosen from a naturally occurring nucleobase, a non-naturally occurring nucleobase, an intercalator, a nucleobase-binding group, a label and H, wherein a series of Qs of adjacent moieties are chosen so as to form one of the following subsequences GGC TTT TAA GGA TTCâ€ƒâ€ƒ(SEQ ID NO: 40) GAT CAA TGC TCG GTTâ€ƒâ€ƒ(SEQ ID NO: 44) CGA CTC CAC ACA AACâ€ƒâ€ƒ(SEQ ID NO: 76) GTC TTT TCG TCC TGCâ€ƒâ€ƒ(SEQ ID NO: 89), or GTC TTA TCG TCC TGCâ€ƒâ€ƒ(SEQ ID NO: 90) â€ƒor a mixture of such probes.