Patent ID: 8034915
Filing Date: 2011-10-11
Classification: C12N

Abstract:
1. An isolated nucleic acid molecule comprising a modified HAP1 DNA-binding domain nucleotide sequence fused to a transcriptional activator domain nucleotide sequence, said modified HAP1 DNA-binding domain nucleotide sequence encoding at least an effective portion of a HAP1 DNA-binding domain, said effective portion of said HAP1 DNA-binding domain determined by measuring binding in an electrophoretic mobility shift assay to a HAP1 upstream activation sequence, characterised in that said modified nucleotide sequence has an A/T base content of less than 45%, wherein said isolated nucleic acid molecule is capable of activating transcription of a second nucleotide sequence in a plant cell when said second nucleotide sequence is operably linked to a HAP1 upstream activation sequence.