Patent ID: 6274347
Filing Date: 2001-08-14
Classification: A61K,A61P,C07K,G01N

Abstract:
A method for producing an antibody that is reactive with cell-surface bound LAM-1 but not reactive with shed LAM-1, wherein the method comprises the steps of contacting antibody producing cells with a fusion protein comprising the extracellular portion of human leukocyte adhesion molecule-1 (LAM-1) fused to the constant region of a human IgG1 constant region, wherein the fusion protein binds to the anti-LAM1-1 monoclonal antibody produced bv the hybridoma cell line having ATCC accession No. HB 10844, and isolating an antibody that binds to the LAM-1 portion of the fusion protein or to cell surface bound LAM-1, but that does not bind to shed LAM-1.