Patent ID: 7638332
Filing Date: 2009-12-29
Classification: A61M,B05B,C12M

Abstract:
1. A method for delivering a biological material using a gene gun, comprising: providing the gene gun comprising a pressurized chamber, a sprayer, a controller valve and a material delivery system; placing a sample solution into the material delivery system, wherein the sample solution comprises at least the biological material; triggering the gene gun and providing a gas through the controller valve to the pressurized chamber until the gas establishes a pressure equal to or lower than 100 psi; releasing the sample solution from the material delivery system, so that the sample solution is accelerated by the gas in the pressurized chamber; and discharging the sample solution out of the sprayer, wherein the sprayer includes a spray nozzle and a spray tube, and the spray nozzle comprises an interior contour, wherein the interior contour of the spray nozzle comprises a diverging part, a converging part and a spray neck positioned between the diverging part and the converging part and connected to the material delivery system, wherein the sample solution is released from the material delivery system around the spray neck of the spray nozzle and is released in a direction perpendicular to a direction of the flow of the gas, and the spray tube is a diverging straight tube, so that the biological material is evenly injected into a target, wherein the biological material is delivered without using micro-carriers or particle carriers.