Patent ID: 9057077
Filing Date: 2015-06-16
Classification: C12N

Abstract:
1. A method for delivery of a toxic agent to a fungal haustorium in an infected plant cell, the method comprising: introducing a vector into a host plant cell, the vector comprising: a fusion gene comprising a nucleotide sequence encoding a RPW8 protein and a nucleotide sequence encoding an antipathogenic protein that is toxic to a specific a haustorium-forming pathogen, wherein the nucleotide sequence encodes for a RPW8 protein consisting of SEQ ID NO. 1 (RPW8.2) or a combination of SEQ ID NO. 1 (RPW8.2) and SEQ ID NO. 8 (RPW8.1) and whereby the RPW8 protein translocates to the EHM for delivery of the antipathogenic protein; and maintaining the cell under appropriate conditions for expression of the fusion protein and the transportation of the expressed RPW8 protein and expressed antipathogenic protein to the extrahaustorial membrane.