Patent ID: 7601821
Filing Date: 2009-10-13
Classification: C12Q

Abstract:
1. A kit for comparing the amount of a target polynucleotide sequence between a first sample and a second sample comprising; A) a first reaction vessel one comprising; a forward primer, wherein the forward primer comprises a target specific portion and an addressable forward primer portion; a reverse primer, wherein the reverse primer comprises a target specific portion and an addressable reverse primer portion, wherein the forward primer, the reverse primer, or both, comprise a first identifying portion; B) a first reaction vessel two comprising; a forward primer, wherein the forward primer comprises a target specific portion and an addressable forward primer portion; a reverse primer, wherein the reverse primer comprises a target specific portion and an addressable reverse primer portion, wherein the forward primer, the reverse primer, or both, comprise a second identifying portion, wherein the identity of the target polynucleotide sequence is encoded with the forward addressable primer portion and the reverse addressable primer portion, and wherein the identity of the first sample is encoded by the first identifying portion and the identity of the second sample is encoded by the second identifying portion; C) a second reaction vessel comprising; a forward address primer and a reverse address primer; wherein the forward addressable primer portion in vessel one is different from the forward addressable primer portion in vessel two; and, D) a PCR master mix comprising; a polymerase, dNTPs, a buffer, a first labeling probe and a second labeling probe, wherein the first labeling probe is complementary to, or complementary to the complement of, the first identifying portion, and wherein the second labeling probe is complementary to, or complementary to the complement of, the second identifying portion.