Patent ID: 6303327
Filing Date: 2001-10-16
Classification: C12N,C12Q

Abstract:
A system for identifying a gene expressed in a cell, comprising:a) a first nucleic acid sequence comprising a first reporter gene encoding a site-specific recombinase, wherein said first reporter gene is not under the control of a promoter; andb) a second nucleic acid sequence comprising, in order from 5' to 3',i. a first promoter,ii. a first target sequence for said recombinase,iii. an intervening nucleotide sequence,iv. a second target sequence for said recombinase, andv. a second reporter gene,wherein said first reporter gene, after being incorporated into an endogenous gene and being transcriptionally activated by an endogenous cellular promoter, is transcribed, and the encoded recombinase is produced, which encoded recombinase activates transcription of the second reporter gene, thereby allowing identification of the transcriptionally active gene under control of the endogenous cellular promoter.