Patent ID: 7794962
Filing Date: 2010-09-14
Classification: C07K,C12N,C12Y,G01N

Abstract:
1. A method, comprising the steps of: (a) stably transforming a plurality of low viscosity filamentous fungi of less than about 200 cP, wherein the fungi are of a genus selected from the group consisting of: (b) culturing the transformed filamentous fungi under conditions conducive to the formation of transferable reproductive elements; (c) separating from one another a plurality of transferable reproductive elements; (d) culturing into monoclonal cultures or clonal colonies the individual transferable reproductive elements, under conditions conducive to expression of the heterologous proteins encoded by the heterologous protein-encoding nucleic acid sequences; (e) screening each individual clonal culture or clonal colony for an expressed protein having the activity or property of interest; (f) isolating one or more individual clonal cultures or clonal colonies that express a protein exhibiting the activity or property of interest; (g) mutating the DNA from the isolated individual clonal cultures or clonal colonies that encodes the protein exhibiting the activity or property of interest; (h) preparing a library of vectors which comprise the mutated DNA sequences obtained in step (i); and (i) repeating steps (a) through (e), until the property or activity of interest is optimized.