Patent ID: 6790623
Filing Date: 2004-09-14
Classification: C12Q,Y10S

Abstract:
A method for the detection of a nucleic acid comprising the steps:a) amplifying the nucleic acid or a part thereof in a reaction mixture with a first primer and a second primer, one of which can hybridize with one strand of the nucleic acid to be detected and the other of which can hybridize with a strand complementary thereto, wherein one of the primers contains a detectable label, to form a labeled extension product and an unlabeled extension product which are complementary to each other; b) contacting a capture probe with the extension products to form a hybridization complex wherein the complex is necessarily a duplex, said hybridization complex comprising the capture probe, the labeled extension product and the unlabeled extension product, wherein the capture probe is selected such that the capture probe hybridizes to only the unlabeled extension product and only the unlabeled extension product hybridizes with the labeled extension product; c) separating the hybridization complex from other components of the reaction mixture; and d) determining the detectable label in the hybridization complex of step b).