Patent ID: 9096676
Filing Date: 2015-08-04
Classification: A61P,C07K

Abstract:
1. A method of producing an antibody specifically recognizing and binding an epitope within the C-terminal part of human MASP-2 comprising the CCP1, CCP2 and serine protease domains, wherein said antibody is capable of inhibiting deposition of C4 substrate added to MBL/MASP-2 complexes present in full human serum obtained from individuals with C4 deposition activity, said method comprising the steps of (a) identifying one or more test antibodies capable of binding an epitope within a polypeptide fragment of human MASP-2 consisting of the CCP1, CCP2 and serine protease domains (aa 293 to 686 of SEQ ID NO:1, comprising: (i) providing human MASP-2 or a polypeptide fragment of human MASP-2 consisting of the CCP1, CCP2 and serine protease domains (aa 293 to 686 of SEQ ID NO:1); (ii) adding a test antibody and a reference antibody to the said MASP-2, wherein either the test antibody or the reference antibody is labeled with a detectable label or both antibodies are labeled with different detectable labels; wherein the reference antibody is selected from the group consisting of: iii) detecting the presence of the detectable label at MASP-2, thereby detecting whether the test antibody is capable of displacing the reference antibody; iv) selecting test antibodies capable of displacing the reference antibody; (b) testing whether antibodies identified in accordance with step (a) are capable of inhibiting deposition of C4 substrate added to MBL/MASP-2 complexes present in full human serum obtained from individuals with C4 deposition activity and (c) selecting antibodies capable of inhibiting C4 deposition in accordance with step (b).