Patent ID: 8119401
Filing Date: 2012-02-21
Classification: A61K,C07K,C12N

Abstract:
1. A method for producing an anti-inflammatory, antigen-non-specific population of lymphocytes overexpressing interleukin-10 (IL-10) polypeptide that are useful for treating a Th1-mediated inflammatory disease in an antigen-independent manner when administered to a subject, the method comprising: (a) modifying at least a portion of a peripheral blood mononuclear cell population from a healthy donor, among which cells lymphocytes are not selected or enriched on the basis of antigen specificity, by introducing into said cells an expression construct that comprises a nucleotide sequence encoding an IL-10 polypeptide; and, (b) recovering, from said modified mononuclear cells, lymphocytes or a subset thereof that overexpress the IL-10 polypeptide, thereby producing said anti-inflammatory, antigen non-specific lymphocyte population.