Patent ID: 6210935
Filing Date: 2001-04-03
Classification: C12N

Abstract:
An isolated DNA fragment comprising a DNA segment having a region encoding a polypeptide contributing to staurosporin biosyntheses, said DNA segment selected from the group consisting ofa) the 35 kb segment of Streptomyces longisporoflavus DSM 10189 inserted in the plasmid vector pNE29 and maintained by the E. coli strain DSM 10188,b) a 2.1 kb segment of said 35 kb insert defined by the pair of Bg/l restriction endonuclease recognition sites set forth in FIG. 2, andc) a chromosomal DNA segment native to a species of Streptomyces capable of staurosporin biosynthesis which hybridizes with a DNA sequence of SEQ ID NO:1 under wash conditions of 55-56.degree. C. in 0.2.times.SSC.