Patent ID: 9096834
Filing Date: 2015-08-04
Classification: C12N,C12P,C12Y

Abstract:
1. A recombinant microorganism comprising a first non-native nucleic acid encoding an acyl-ACP thioesterase from a higher plant species or from a prokaryotic organism and a second non-native nucleic acid encoding a lysophosphatidic acid acyltransferase (LPAAT) from a prokaryotic organism, wherein the microorganism produces a fatty acid product, wherein the thioesterase and the LPAAT have complementary acyl-ACP substrate preferences, and wherein the complementary acyl-ACP substrate preferences are selected from the group consisting of: (1) the thioesterase has a substrate preference for one or more acyl substrates having an acyl chain length selected from the group consisting of C12, C14, and C16, and the LPAAT has a substrate preference for substrates having a C18 acyl chain length; (2) the thioesterase has a substrate preference for one or more acyl substrates having an acyl chain length of C12 and C14 and the LPAAT has a substrate preference for substrates having a C16 or C18 acyl chain length; (3) the thioesterase has a substrate preference for one or more acyl substrates having an acyl chain length of C12 and the LPAAT has a substrate preference for one or more substrates having an acyl chain length selected from the group consisting of C14, C16, and C18 acyl chain length; and (4) the thioesterase has a substrate preference for one or more acyl substrates having an acyl chain length selected from the group consisting of C12, C14, C16, and C18, and the LPAAT has a substrate preference for substrates having a C20, C22, or C24 acyl chain length.