Patent ID: 6781033
Filing Date: 2004-08-24
Classification: C12N

Abstract:
A method for obtaining a transplastomic plant, wherein the method comprises the steps of:a) introducing into a plastid of a plant cell a recombinant nucleic acid construct comprising regions of plastid homology, a promoter functional in a plant cell plastid, a nucleic acid sequence encoding a glyphosate resistant EPSPS, and a transcriptional termination region functional in a plant cell plastid to produce a transformed plant cell, b) placing said transformed plant cell on a first culture medium comprising a sublethal amount of glyphosate for a period of time sufficient to permit said plant cell to replicate to produce replicated plant cells, and c) placing said replicated plant cells on a second culture medium comprising a lethal concentration of glyphosate for a period of time sufficient to select plant cells capable of growing in the presence of glyphosate; and d) regenerating a transplastomic plant from said plant cells that grow in said second culture medium.