Patent ID: 6103487
Filing Date: 2000-08-15
Classification: A61K,C12Q,G01N,Y10S,Y10T

Abstract:
An assay for identifying a compound that inhibits geranylgeranyl-protein transferase type I activity, comprising:a) reacting a protein or peptide substrate comprising a CAAX.sup.G motif with geranylgeranyl pyrophosphate and geranylgeranyl-protein transferase type I in the presence of a test compound and further in the presence of a modulating anion;b) detecting whether the geranylgeranyl residue is incorporated into the protein or peptide substrate, in which the ability of the test compound to inhibit geranylgeranyl-protein transferase type I activity is indicated by a decrease in the incorporation of the geranylgeranyl residue into the protein or peptide substrate as compared to the amount of the geranylgeranyl residue incorporated into the protein or peptide substrate in the absence of the test compound.