Patent ID: 7741098
Filing Date: 2010-06-22
Classification: A01K,C12N

Abstract:
1. Plasmid vector for expression in Caenorhabditis elegans and in Escherichia coli comprising in the 5′ to 3′ direction of transcription operably linked to each other a heat shock promoter nucleotide sequence, a synthetic intron nucleotide sequence containing a Shine-Dalgarno sequence, optionally a nucleotide sequence coding for a nuclear localization signal or a secretion signal, a nucleotide sequence coding for a recognizable tag, optionally a nucleotide sequence coding for a fluorescent protein, a nucleotide sequence coding for a protease cleavage site, a multiple cloning site containing a nucleic acid molecule or a nucleotide sequence coding for a eukaryotic protein, and a nucleotide sequence coding for termination of translation so as to express a eukaryotic protein or nucleic acid molecule in wherein the synthetic intron nucleotide sequence contains the Shine-Dalgarno sequence AGGAG, the nucleotide sequence coding for a nuclear localization signal is SEQ ID NO: 3, the sequence coding for a recognizable tag is a sequence coding for a 6-Histidine (His), 10-His or 12-His tag, the nucleotide sequence coding for a fluorescent protein is a nucleotide sequence coding for the green fluorescent protein with the sequence SEQ ID NO: 8, the nucleotide sequence coding for a protease cleavage site is a sequence coding for a Tobacco Etch Virus (TEV) protease cleavage site.