Patent ID: 7858576
Filing Date: 2010-12-28
Classification: A61K,A61P,C07K,C12N,C12Y

Abstract:
1. A method of targeting a therapeutic enzyme to a lysosome, the method comprising: (a) providing a targeted therapeutic comprising a therapeutic enzyme and a lysosomal-targeting domain that binds the cation-independent mannose-6-phosphate receptor in a mannose-6-phosphate-independent manner; wherein the lysosomal-targeting domain comprises a mature human IGF-II; (b) contacting the targeted therapeutic with a cell deficient in a lysosomal enzyme, wherein the cell expresses the cation-independent mannose-6-phosphate receptor on the surface, thereby targeting the therapeutic enzyme to the lysosome as a result of the binding between the lysosomal-targeting domain and the cation-independent mannose-6-phosphate receptor.