Patent ID: 7899624
Filing Date: 2011-03-01
Classification: G01N

Abstract:
1. A tissue cytometry method for analyzing cells in tissue section comprising: performing light microscopic image analysis on paraffin embedded tissue section comprising: Multi-stage thresholding and morphologic processing of said digital image comprising the steps of: separating from said digital image of red, green, and blue channels the respective image planes containing gray-scale values of each channel contained in said digital image; applying dynamic thresholding to said gray-scale image planes to yield thresholded masks corresponding to red, green, and blue channels, respectively; combining said masks for red and blue planes using a logical OR operation to create a combined mask; masking said digital image with the said combined mask to get a raw working image color herein named preliminary raw working image; further analyzing said preliminary raw working image to get immunostained cells comprising the steps: assigning final immunostained objects and final non-immunostained objects as final positive cell objects and final negative cell objects, respectively.