Patent ID: 8183217
Filing Date: 2012-05-22
Classification: C12N,C12Y

Abstract:
1. A method for expressing an unpolyadenylated hairpin RNA in an animal cell, the method comprising the step of providing to an animal cell in culture a DNA comprising a promoter operably linked to a target specific DNA region encoding the unpolyadenylated hairpin RNA, wherein the unpolyadenylated hairpin RNA comprises a target specific sense nucleotide sequence and a target specific antisense nucleotide sequence, wherein the target specific antisense nucleotide sequence consists of about 20 consecutive nucleotides in a sequence identical to the sequence of the complement of a part of an RNA molecule transcribed or produced from a nucleic acid of interest in the animal cell, wherein the target specific sense nucleotide sequence consists of about 20 consecutive nucleotides in a sequence identical to the sequence of the part of the RNA molecule transcribed or produced from the nucleic acid of interest, and wherein the target specific sense nucleotide sequence and the target specific antisense nucleotide sequence are complementary to each other, so as to form in the nucleus of the animal cell the unpolyadenylated hairpin RNA.