Patent ID: 8559021
Filing Date: 2013-10-15
Classification: G01N,G02B

Abstract:
1. A method of three-dimensionally localizing light emitting marker entities of unknown orientation and unknown position in a sample, the method comprising: imaging light emitted by each single marker entity in at least two different ways onto at least one detection plane which corresponds to a focal plane in the sample resulting in at least two images of each marker entity; separately determining virtual x- and y-positions in parallel to the focal plane of each marker entity from the emitted light intensity distribution over each image of each marker entity; determining a z-position of each marker entity normal to the focal plane from the emitted light intensity distributions over the images of each marker entity; and determining real x- and y-positions of each marker entity in parallel to the focal plane based on its virtual x- and y-positions and on its z-position.