Patent ID: 7179598
Filing Date: 2007-02-20
Classification: C12Q

Abstract:
1. A method of detecting a chromosome translocation associated with leukemia, the method comprising: (a) amplifying a target nucleic acid sequence from a biological sample; (b) hybridizing a first oligonucleotide comprising the sequence set forth in SEQ ID NO: 40 to the amplified target under conditions in which the first oligonucleotide specifically hybridizes to a first region of the translocation, wherein the first oligonucleotide comprises a first capture tag; (c) hybridizing a second oligonucleotide comprising the sequence set forth in SEQ ID NO: 41 to the amplified target under conditions in which the second oligonucleotide specifically hybridizes to a second region of the translocation, wherein the second oligonucleotide comprises a second capture tag; (d) extending the first and second hybridized oligonucleotide sequences to produce a first and a second labeled extended oligonucleotide; (e) hybridizing the first and second oligonucleotides to a first and a second address tag on a solid support under conditions in which the first and second address tags specifically hybridize to the first and second capture tags; and (f) detecting the presence of the first and second labeled extended oligonucleotides on the solid support, thereby detecting the presence of the chromosome translocation associated with leukemia.