Patent ID: 6294347
Filing Date: 2001-09-25
Classification: A61K,A61P,C07K,C12N,G01N

Abstract:
A method for detecting immune complexes in a sample, comprising the steps of:providing host cells transformed with a vector carrying a cDNA insert encoding a Fc.gamma.RIII molecule, wherein the Fc.gamma.RIII molecule is expressed on the surface of the transformed host cells and is expressed form the group consisting of the following sequences:Arg-Thr-Glu-Asp-Leu-Pro-Lys-Ala-Val-Val-Phe-Leu- Glu-Pro-Gln-Trp-Tyr-Arg-Val-Leu-Glu-Lys-Asp-Ser- Val-thr-Leu-Lys-Cys-Gln-gly-Ala-Tyr-Ser-Pro-Glu- Asp-Asn-Ser-Thr-Gln-Trp-Phe-his-Asn-Glu-Asn-Leu- Ile-Ser-Ser-Gln-Ala-Ser-Ser-Tyr-Phe-Ile-Asp-Ala- Ala-Thr-Val-Asp-Asp-Ser-Gly-Glu-tyr-Arg-Cys-Gln- Thr-Asn-Leu-Ser-Thr-Leu-Ser-Asp-Pro-Val-Gln-Leu- Glu-Val-his-Val-Gly-Trp-Leu-Leu-Leu-Gln-Ala-Pro- Arg-Trp-Val-Phe-Lys-Glu-Glu-Asp-Pro-Ile-His-Leu- Arg-Cys-His-Ser-Trp-Lys-Asn-Thr-Ala-Leu-His-Lys- Val-Thr-Tyr-Leu-Gln-Asn-Gly-Lys-Asp-Arg-lys-Tyr- Phe-His-His-Asn-Ser-Asp-Phe-His-Ile-Pro-Lys-Ala- Thr-Leu-Lys-Asp-Ser-Gly-Ser-Tyr-Phe-Cys-Arg-Gly- Leu-Val-Gly-Ser-Lys-Asn-Val-Ser-Ser-Glu-Thr-Val- Asn-Ile-Thr-Ile-Thr-gln-Gly-Leu-Ala-Val-Ser-Thr- Ile-Ser-Ser-Phe-Ser-Pro-Pro-Gly; and Arg-Thr-Glu-Asp-Leu-Pro-Lys-Ala-Val-Val-Phe-Leu- Glu-Pro-Gln-Trp-Tyr-Arg-Val-Leu-glu-Lys-Asp-Ser- Val-thr-Leu-Lys-Cys-Gln-gly-Ala-Tyr-Ser-Pro-Glu- Asp-Asn-Ser-Thr-gln-Trp-Phe-His-Asn-Glu-Ser-Leu- Ile-Ser-Ser-Gln-Ala-Ser-Ser-Tyr-Phe-Ile-Asp-Ala- Ala-Thr-VAl-Asp-Asp-Ser-Gly-Glu-Tyr-Arg-Cys-Gln- Thr-Asn-Leu-Ser-Thr-Leu-Ser-Asp-Pro-Val-Gln-Leu- Glu-Val-His-Ile-Gly-Trp-Leu-Leu-Leu-Gln-Ala-Pro- Arg-Trp-Val-Phe-Lys-glu-Glu-Asp-Pro-Ile-his-Leu- Arg-Cys-His-Ser-Trp-Lys-Asn-thr-Ala-Leu-His-Lys- Val-Thr-Tyr-Leu-gln-Asn-Gly-Lys-Gly-Arg-Lys-Tyr- Phe-His-His-Asn-Ser-Asp-Phe-tyr-Ile-Pro-Lys-Ala- Thr-Leu-Lys-Asp-Ser-Gly-Ser-Tyr-Phe-Cys-Arg-Gly- Leu-Phe-Gly-Ser-Lys-Asn-Val-Ser-Ser-glu-Thr-Val- Asn-Ile-Thr-Ile-Thr-Gln-Gly-Leu-Ala-Val-Ser-Thr- Ile-Ser-Ser-Phe-Phe-Pro-Pro-Gly;exposing the transformed host cells to a sample so that any immune complex present in the sample adheres to Fc.gamma.RIII molecules expressed on the surface of the transformed host cells; labeling any immune complex adhering to Fc.gamma.RIII molecules on the surfaces of the transformed host cells; and measuring the amount of labeled immune complex adhering to the transformed host cells.