Patent ID: 6204008
Filing Date: 2001-03-20
Classification: C07K

Abstract:
A process for producing an Ala-Pro dipeptide, which comprises:(a) transfecting a DNA expression vector into suitable host cells wherein said DNA expression vector comprises a DNA molecule which encodes an oligopeptide or polypeptide containing multiple direct repeats of said Ala-Pro dipeptide;(b) culturing said host cells under conditions suitable for expression of said oligopeptide or polypeptide from said DNA expression vector;(c) cleaving said expressed oligopeptide or polypeptide with an enzyme capable of cleaving said Ala-Pro dipeptides from each other, thereby releasing said Ala-Pro dipeptides, wherein said enzyme is selected as a combination of at least an exo- and an endopeptidase selected from the group consisting of: factor X, Lactobacillus sp. prolypeptidase, Lactobacillus helveticus prolypeptidase, Flavobacterium meningosepticum prolypeptidase, and Xanthomonas maltophilia prolypeptidase; and,(d) isolating and purifying said Ala-Pro dipeptides.