Patent ID: 6458585
Filing Date: 2002-10-01
Classification: A61K,C12N

Abstract:
A method for producing a culture enriched for human dendritic cells for therapeutic purposes, said method comprising:(a) providing mononuclear cells from peripheral or umbilical cord blood or bone marrow of an individual human donor, wherein said mononuclear cells comprise dendritic cell precursors, (b) culturing said mononuclear cells in a culture container in a culture medium that supports mononuclear cell culture, said medium further containing an effective amount of an additive that differentiates dendritic cell precursors into dendritic cells, wherein said additive is prostaglandin E, and (c) maintaining the culture of said mononuclear cells to produce a culture enriched for dendritic cells as evidenced by at least about 2.5% of total cells in the culture exhibiting dendritic cell processes.