Patent ID: 7132248
Filing Date: 2006-11-07
Classification: A01K,A61K,A61P,C07K,C12P

Abstract:
1. A process for determining whether a chemical compound specifically binds to and activates a human or a rat galanin receptor (GALR2), which comprises contacting cells producing a second messenger response and transfected with DNA encoding and expressing on their cell surface the human or the rat galanin receptor (GALR2), or a membrane preparation from such cells, wherein such cells prior to being transfected with such DNA do not normally express the human or rat galanin receptor (GALR2), with the chemical compound under conditions suitable for activation of the human or the rat galanin receptor (GALR2), and measuring the second messenger response in the presence and in the absence of the chemical compound, a change in second messenger response in the presence of the chemical compound indicating that the chemical compound activates the human or the rat galanin receptor (GALR2), wherein the human galanin receptor (GALR2) has an amino acid sequence identical to that (a) shown in SEQ ID NO: 28, (b) expressed by COS-7 cells transfected with plasmid BO29 (ATCC Accession No. 97735) or (c) expressed by COS-7 cells transfected with plasmid BO39 (ATCC Accession No. 97851) and the rat galanin receptor (GALR2) has an amino acid sequence identical to that (a) shown in SEQ ID NO: 8 or (b) expressed by LM(tk−) cells, COS-7 cells or CHO cells transfected with (i) plasmid K985 (ATCC Accession No. 97426) or (ii) plasmid K1045 (ATCC Accession No. 97778).