Patent ID: 8932808
Filing Date: 2015-01-13
Classification: A61P,C12Q

Abstract:
1. A method for detecting a graft tolerant phenotype in a human subject, said method comprising: (a) assaying gene expression levels of chemokine (C-C motif) ligand 20 (CCL20), complement component 1s (C1s), and thymidine kinase 1 (TK1) genes in a blood sample from the subject to obtain a test expression profile, wherein assaying gene expression levels is performed using a method comprising at least one of: quantitative PCR, and a microarray; (b) comparing the test expression profile with a reference expression profile, wherein the reference expression profile comprises gene expression levels of CCL20, C1s, and TK1 that are indicative of either: (i) a renal graft tolerant phenotype; or (ii) a renal graft intolerant phenotype; and (c) detecting gene expression levels of CCL20, C1s, and TK1 in the test expression profile that are the same as the gene expression levels of CCL20, C1s, and TK1 in the reference expression profile that is indicative of a renal graft tolerant phenotype and determining that the human subject has a renal graft tolerant phenotype; or detecting gene expression levels of CCL20, C1s, and TK1 in the test expression profile that are increased compared to the gene expression levels of CCL20, C1s, and TK1 in the reference expression profile that is indicative of a renal graft intolerant phenotype and determining that the human subject has a renal graft tolerant phenotype.