Patent ID: 6072049
Filing Date: 2000-06-06
Classification: A61K,A61P,C07K,C12N,Y02A,Y10S

Abstract:
A recombinant DNA molecule comprising an expression control sequence operatively linked to first and second DNA sequences in the same reading frame, the first and second DNA sequences each encoding for discrete regions of a single polypeptide expressed by the recombinant DNA molecule,wherein:the first DNA sequence comprises a nucleotide sequence encoding a first amino acid sequence, wherein the first amino acid sequence comprises all or a portion of the sequence of amino acids 1 to 47 of an HBV pre-S1 peptide and exhibits the antigenicity of an HBV pre-S1 epitope; andthe second DNA sequence comprises a nucleotide sequence encoding a second amino acid sequence, wherein the second amino acid sequence comprises all or a portion of an HBV surface antigen peptide having the capacity to be assembled into particles; andwherein the polypeptide:does not comprise the entire sequence of amino acids of an HBV pre-S1 peptide;exhibits the antigenicity of the HBV pre-S1 epitope; andwhen produced by the recombinant DNA molecule in cultured host cells, retains the capacity of the second amino acid sequence to be assembled into particles.