Patent ID: 7776587
Filing Date: 2010-08-17
Classification: C07K,C12N

Abstract:
1. A polynucleotide sequence encoding a gene switch comprising: (a) a first gene expression cassette comprising a polynucleotide sequence that encodes a first polypeptide comprising: (b) a second gene expression cassette comprising a polynucleotide sequence that encodes a second polypeptide comprising: wherein the transactivation domain is from a nuclear receptor other than an ecdysone receptor, a retinoid X receptor, or an ultraspiracle receptor, wherein the first and second polypeptides dimerize wherein at least one of the first polypeptide and the second polypeptide does not contain the A and B domains of the corresponding ecdysone receptor or retinoid X receptor and wherein said gene switch is more sensitive to a diacylhydrazine ligand than to a steroid ligand when expressed in a mammalian cell.