Patent ID: 8962267
Filing Date: 2015-02-24
Classification: C12Q,G01N

Abstract:
1. A method for predicting determining the response of a patient diagnosed with non-small cell lung cancer (NSCLC) to a medicament, comprising the steps of: (a) providing a solid support having immobilized thereupon at least the 20 protein kinase substrates consisting of SEQ ID NO: 1, 2, 4, 6, 8, 9, 11, 13, 26, 27, 32, 37, 45, 49, 61, 65, 72, 75, 76 and 79; a lysate sample obtained from the NSCLC tumor from said patient; and a medicament; (b) measuring the kinase activity of the lysate sample in the presence and in the absence of said medicament by contacting a first part of said lysate sample with said at least 20 protein kinase substrates in the presence of said medicament, and by contacting a second part of said lysate sample with said at least 20 protein kinase substrates in the absence of said medicament, thereby providing a phosphorylation profile of said lysate sample in contact with and in the absence of said medicament, respectively; and (c) determining from said phosphorylation profiles in contact with and in the absence of said medicament the lysate sample differential phosphorylation level in response to the medicament, said differential phosphorylation level determining the response of said patient to said medicament.