Patent ID: 8232107
Filing Date: 2012-07-31
Classification: A61K,G01N,Y02P,Y10T

Abstract:
1. A method for measuring the in vivo metabolism of a protein in a subject, the protein being synthesized in the central nervous system, the method comprising: (a) administering a labeled moiety to the subject, the labeled moiety being capable of crossing the blood brain barrier and incorporating into the protein as the protein is synthesized in the central nervous system of the subject; (b) obtaining a biological sample from the subject, the biological sample comprising a protein fraction labeled with the moiety and a protein fraction not labeled with the moiety; and (c) detecting the amount of labeled protein and the amount of unlabeled protein by mass spectrometry, wherein the ratio of labeled protein to unlabeled protein is directly proportional to the metabolism of the protein in the subject, wherein the protein is selected from the group consisting of apolipoprotein E, apolipoprotein J, synuclein, soluble amyloid precursor protein, Tau, alpha-2 macroglobulin, S100B, myelin basic protein, an interleukin, and TNF.