Patent ID: 9169506
Filing Date: 2015-10-27
Classification: C08B,C12N,C12P,C12Y

Abstract:
1. A process for producing poly alpha-1,3-glucan comprising: (a) contacting water, sucrose, a polar organic solvent, and a bacterial glucosyltransferase enzyme in a solution, wherein the glucosyltransferase enzyme synthesizes poly alpha-1,3-glucan, wherein a poly alpha-1,3-glucan is produced having a reduced molecular weight compared to the molecular weight of the poly alpha-1,3-glucan produced in the absence of the polar organic solvent, wherein the glucosyltransferase enzyme comprises an amino acid sequence that is at least 97% identical to SEQ ID NO: 12, SEQ ID NO: 2, SEQ ID NO: 32, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 14, SEQ ID NO: 18, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, or SEQ ID NO:30; and (b) optionally, isolating the poly alpha-1,3-glucan produced in step (a); wherein the polar organic solvent is selected from the group consisting of acetonitrile, dimethyl sulfoxide, acetone, N,N-dimethylformamide, N,N-dimethylacetamide, tetrahydrofuran, propylene carbonate, sulfolane, hexamethylphosphoramide, 1,3-dimethyl-2-imidazolidinone, nitromethane, butanone, methanol, methyl formamide, ethanol, isopropanol, 1-propanol, tert-butyl alcohol, formamide, n-butanol, ethylene glycol, 2-methoxyethanol, 1-methoxy-2-propanol, glycerol, 1,2-propanediol, and 1,3-propanetriol.