Patent ID: 6500617
Filing Date: 2002-12-31
Classification: C07K,C12N,Y02A

Abstract:
A method of obtaining an optimized recombinant pest resistance gene, the method comprising:(1) recombining a plurality of forms of a nucleic acid which comprise segments derived from a gene that confers resistance to a pest upon a plant in which the gene is effectively expressed, wherein the plurality of forms of the nucleic acid differ from each other in two or more nucleotides, to produce a library of recombinant genes, wherein the library includes at least one optmized recombinant pest resistance gene that confers resistance to a pest upon a plant in which the gene is effectively expressed, wherein the optimized recombinant pest resistance gene exhibits improved pest resistance capability compared to a non-recombinant pest resistance gene; (2) expressing members of the library of recombinant genes, thereby producing polypeptides encoded by the expressed members of the library of recombinant genes; and (3) assaying the polypeptides for the ability to confer pest resistance upon a plant, thereby identifying the optimized recombinant pest resistance gene; wherein the optimized recombinant pest resistance gene is identified by observation of the improved pest resistance capability of the polypeptide encoded by the optimized recombinant pest resistance gene compared to a polypeptide encoded by a non-recombinant pest resistance gene.