Patent ID: 7700275
Filing Date: 2010-04-20
Classification: C12Q

Abstract:
1. A method for detecting the presence of a target nucleic acid sequence in a sample, the method comprising: performing nucleic acid amplification on the sample in the presence of (a) a DNA duplex binding agent in an unbound state, (b) a nucleic acid polymerase and (c) a reagent comprising an amplification primer which can hybridise to the target sequence when in single stranded form and which is connected at its 5′ end to a probe which carries a label by way of a chemical linking group, the labelled probe being of a sequence such that the labelled probe hybridizes to a complementary region in an amplification product, and wherein the label is able to absorb fluorescence from or donate fluorescent energy to the DNA duplex binding agent; and monitoring fluorescence of the sample.