Patent ID: 7435557
Filing Date: 2008-10-14
Classification: A01N,C12N,C12Q,C12Y,G01N

Abstract:
1. A method for identification of a candidate compound as an inhibitor of GTP cyclohydrolase I or II comprising the steps of: a) adding GTP or GTP analog, NAD+ and formate dehydrogenase to a first sample comprising GTP cyclohydrolase I or II; b) adding formate, NAD+ and formate dehydrogenase to a second sample comprising GTP cyclohydrolase I or II; c) adding to the first sample and to the second sample, a candidate compound; d) determining the production of NADH in the first sample and the second sample, and e) selecting the candidate compound that shows inhibition of NADH production in the presence of GTP and no inhibition of NADH production in the presence of formate, wherein inhibition of NADH production in the first sample and no inhibition of NADH production in the second sample identifies that the candidate compound is an inhibitor of GTP cyclohydrolase I or II and not an inhibitor of formate dehydrogenase.