Patent ID: 6410498
Filing Date: 2002-06-25
Classification: C11D,C12N,C12Y,D06M

Abstract:
A modified enzyme which comprises a catalytically active amino acid sequence of a transferase linked to an amino acid sequence comprising a cellulose binding domain via a linking region selected from the group consisting of, Humicola insolens family 45 cellulase linker, NifA gene of Klebsiella pneumonia-CiP linker, E. coli OmpA gene-CiP linker, E3 cellulase Thermomonospora fusca linker, CenA cellulase linker, polyethylene glycol derivatives, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, N-ethyl-5-phenylisoaxolium-3-sulphonate, N-ethoxycarbonyl-2-ethoxy 1,2,dihydroquinoline or glutaraldehyde, crosslinkers and combinations thereof; wherein said transferase is selected from the group consisting of: EC 2.1.1.15, EC 2.1.1.18, EC 2.1.2.1, EC 2.1.2.4, EC 2.2.1.3, EC 2.3.1.3, EC 2.3.1.18, EC 2.3.1.57, EC 2.3.1.75, EC 2.3.1.79, EC 2.3.1.84, EC 2.3.1.88, EC 2.3.1.96, EC 2.3.1.142, EC 2.4.1.2, EC 2.4.1.4, EC 2.4.1.5, EC 2.4.1.9, EC 2.4.1 .10, EC 2.4.1.11, EC 2.4.1.12, EC 2.4.1.13, EC 2.4.1.16, EC 2.4.1.18, EC 2.4.1.21, EC 2.4.1 24, EC 2.4.1.25, EC 2.4.1.29, EC 2.4.1.34, EC 2.4.1.35, EC 2.4.1.49, EC 2.4.1.67, EC 2.4.1.71, EC 2.4.1.75, EC 2.4.1.82, EC 2.4.1.90, EC 2.4.1.93, EC 2.4.1.99, EC 2.4.1.100, EC 2.4.1.113, EC 2.4.1.121, EC 2.4.1.125, EC 2.4.1.140, EC 2.4.1.161, EC 2.4.1.168, EC 2.4.1.169, EC 2.4.1.183, EC 2.5.1.10, EC 2.5.1.20, EC 2.6.1, 2.3.2.13, xyloglucan endotransglycosylase (XET), endo-xyloglucan transferase (EXGT) and combinations thereof.