Patent ID: 6737269
Filing Date: 2004-05-18
Classification: C12N

Abstract:
A recombinant multi-state genetic oscillator comprising:(a) a first nucleic acid construct comprising a first inducible promoter operably associated with a first gene encoding a first protein; (b) a second nucleic acid construct comprising a promoter operably associated with a second gene encoding a second protein and to a third gene encoding a third protein, wherein transcription from the promoter is active in the absence of a repressor; and (c) a third nucleic acid construct comprising a second inducible promoter operably associated with a fourth gene encoding a fourth protein, wherein (i) the first protein, when produced, is capable of repressing transcription from the promoter of part (b), (ii) the second protein, when produced, is capable of repressing transcription from the first inducible promoter, (iii) the third protein, when produced, is capable of increasing transcription from the second inducible promoter, and (iv) the fourth protein, when produced, is capable of increasing transcription from the first inducible promoter.