Patent ID: 7501260
Filing Date: 2009-03-10
Classification: A61P,G01N

Abstract:
1. A method for determining efficacy of chemotherapeutic agents for a patient prior to treatment for cancer, comprising the steps of: mechanically separating a cancer patient's tumor tissue specimen into multicellular particulates, wherein the patient's tumor tissue specimen comprises malignant cells whose growth and morphology may be monitored in a monolayer culture, and wherein the multicellular particulates are not dissociated cell suspensions; monitoring cell growth and morphology of a non-confluent monolayer from said multicellular particulates, wherein the non-confluent monolayer comprises malignant cells without overgrowth of fibroblasts; suspending cells of said monolayer in a plurality of segregated sites; and screening the cells in the segregated sites for sensitivity to a plurality of chemotherapeutic agents indicated for treatment of said cancer, thereby determining the efficacy of each agent for the patient prior to treatment.