Patent ID: 6043093
Filing Date: 2000-03-28
Classification: C07K,C12N,C12Q

Abstract:
A method for directed evolution of a putative molecule into a molecule that interacts with a recognition sequence, said method comprising the steps of:(a) expressing multiple copies of a putative molecule or a multiplicity of different putative molecules in a population of host cells containing a growth factor, a modulation moiety and said recognition sequence; and(b) subjecting said population of host cells to mutagenizing conditions and imposing selection conditions to select for those host cells and/or replicators having modified growth as a result of an interaction between said molecule that interacts with said recognition sequence, said modulation moiety and said recognition sequence, such that at least one replicator and/or cell in said population undergoes at least one cycle of division and/or replication under said conditions;wherein said putative molecule or multiplicity of different putative molecules evolve into said molecule that interacts with said recognition sequence.