Patent ID: 8026050
Filing Date: 2011-09-27
Classification: C07H,C07K,C12N,C12Q

Abstract:
1. A method of modifying a polynucleotide, said method comprising: (a) providing in combination: (b) reversibly hybridizing under isothermal conditions said polynucleotide and said first oligonucleotide, wherein said first oligonucleotide, when hybridized to said polynucleotide, is cleaved by said 5′-nuclease as a result of the presence of said polynucleotide to provide: (i) a first fragment that is substantially non-hybridizable to said polynucleotide, or a first fragment including said 5′ portion and no more than one nucleotide from the 5′ end of said 3′ portion, and (ii) a second fragment that is 3′ of said first fragment with reference to said first oligonucleotide and is substantially hybridizable to said polynucleotide, thereby modifying said polynucleotide.