Patent ID: 7566454
Filing Date: 2009-07-28
Classification: A61K,C07K,C12N,Y10S

Abstract:
1. A method for inducing an immune response to one or more influenza polypeptides in a subject, the method comprising: administering to the subject a composition comprising (a) a nucleic acid molecule comprising a sequence encoding an influenza type hemagglutinin (HA) polypeptide of an H1 subtype or an antigenic fragment thereof, wherein the sequence is at least 95% identical to SEQ ID NO:1, or comprises at least 30 contiguous nucleotides that are identical to 30 contiguous nucleotides of SEQ ID NO:1, and wherein the sequence is codon-optimized for expression in a mammalian cell; (b) a mammalian promoter operably linked to the nucleic acid molecule, wherein the promoter directs transcription of mRNA encoding the influenza polypeptide; and (c) a mammalian polyadenylation signal operably linked to the nucleic acid molecule, wherein the composition is administered in an amount sufficient for the sequence to express the influenza polypeptide at a level sufficient to induce an immune response in the subject.