Patent ID: 7504496
Filing Date: 2009-03-17
Classification: C07D,C07H,C09B,G01N,Y10S

Abstract:
1. A labeled nucleoside/tide or nucleoside/tide analog comprising a rhodamine dye conjugated by a linker (L′) to a nucleoside/tide or nucleoside/tide analog (NUC), wherein: the rhodamine is a rhodamine-type parent xanthene having attached to the xanthene C9 carbon a phenyl group, wherein the phenyl group is further substituted with (i) a carboxy group or a sulfonate group or a salt thereof; (ii) one to three substituted or unsubstituted aminopyridinium groups; and (iii) a substituted or unsubstituted alkylthio or arylthio group; and the nucleoside/tide or nucleoside/tide analog and linker taken together comprise the structure: wherein: B is a nucleobase selected from a purine, a 7-deazapurine, an 8-aza,7-deazapunne, a pyrimidine, a normal nucleobase and an analog of a normal nucleobase; L′ is the linker comprising one to twenty carbon atoms; R R where a is an integer from 0 to 2, and a phosphate ester analog, or a salt thereof.