Patent ID: 7888490
Filing Date: 2011-02-15
Classification: A61K,A61P,C07K

Abstract:
1. A recombinant DNA construct for expressing mutagenized toxin protein of Bacillus anthracis comprising: A) an expression vector, and B) a DNA fragment comprising a gene encoding mutant type Protective Antigen (PA), mutant type Lethal Factor (LF) or mutant type Edema Factor (EF), or a combination thereof wherein the DNA fragment comprises a non-native sequence that results from amplifying at least one of (i) a gene encoding native Protective Antigen (PA) with a primer selected from the group consisting of SEQ ID NO: 1, 2, 3, 4, 5, and 6; (ii) amplifying a gene encoding native Lethal Factor (LF) with a primer selected from the group consisting of SEQ ID NO: 20, 21, 22, 23, 24, 25 and 26; and (iii) amplifying a gene encoding native Edema Factor (EF) with a primer selected from the group consisting of SEQ ID NO: 27, 28, 29 and 30.