Patent ID: 7829300
Filing Date: 2010-11-09
Classification: G01N

Abstract:
1. A method of measuring the activation of a G(alpha)i-and/or G(alpha)o-coupled receptor in cells that express at least one G(alpha)q-coupled receptor, said method comprising: (a) simultaneously treating the cells with an amount of an agonist of the G(alpha)q-coupled receptor such that a sub-threshold activity is obtained, and with an agonist of the G(alpha)i- and/or G(alpha)o-coupled receptor, and (b) measuring the cytoplasmic Ca wherein the amount of the agonist of the G(alpha)q-coupled receptor that produces a sub-threshold activity is that amount at which the ratio of: (i) the result of the measurement of the cytoplasmic Ca to (ii) the result of the measurement of the cytoplasmic Ca does not exceed about 1:20.