Patent ID: 6677163
Filing Date: 2004-01-13
Classification: C07H,G01N,Y10S

Abstract:
A method for immobilising a bio-molecule on a silicon substrate, the method comprising the steps:(A) providing Siâ€”H groups at a surface of the silicon substrate; (B) attaching a bi-functional linker molecule having a coupling group that is unreactive with said silicon substrate and with bio-molecules until activation or deprotection and a CH2&boxH;CHâ€” group to the silicon substrate by reacting a Siâ€”H group at said surface with the CH2&boxH;CHâ€” group of the linker molecule to form a covalent Siâ€”C bond between said silicon substrate and said linker molecule, thus forming an attached linker molecule; (C) modifying said coupling group of said attached linker molecule by activation or deprotection to make said coupling group ready for reaction with a bio-molecule to form an attached linker molecule having an reactive coupling group; and (D) covalently reacting said attached linker molecule having a reactive coupling group with a bio-molecule selected from the group consisting of a DNA, RNA, protein and carbohydrate, to immobilise said bio-molecule on said silicon substrate.