Patent ID: 8431339
Filing Date: 2013-04-30
Classification: B01L,C12N,G01N

Abstract:
1. An integrated fluidic component for purification of nucleic acid molecules, wherein the component comprises: at least one sample chamber for receiving a sample containing the nucleic acid molecules, the sample being a cell lysate or cell mixture; at least one electrophoretic gel separation section for separating the nucleic acid molecules from other components of the sample; at least one collecting chamber for receiving the purified nucleic acid molecules, wherein the collecting chamber has at least one integrated receptor device for detecting the presence and/or the concentration of the purified nucleic acid molecule; and an optional side channel for incorporating the gel into the electrophoretic gel separation section, wherein the electrophoretic gel separation section is directly connected with the sample chamber, so that the sample is purified directly through the electrophoretic separation section, wherein the gel separation section is adapted so as to separate ribonucleic acid molecules from the sample and the electrophoretic gel separation section comprises phase guides creating local changes of capillary forces for controlling the progression and reproducible shaping of the gel front during gel incorporation into the electrophoretic gel separation section, wherein the phase guides comprise thin strips which locally change the capillary forces into the electrophoretic gel separation section and create pressure barriers which control the gel spatially within the gel separation section.