Patent ID: 6582907
Filing Date: 2003-06-24
Classification: G01N,Y10S,Y10T

Abstract:
A screening method for identifying a test compound that binds to a target species, the method comprising:incubating at least one test mixture under isothermal denaturing conditions, each test mixture comprising at least one test compound, at least one reporter molecule, and at least one target species, wherein the isothermal denaturing conditions are effective to cause at least a portion of the target species to denature to a measurable extent; detecting a denaturation signal of each target species in the presence of the at least one test compound by monitoring the diffusion properties of the target molecule using fluorescence correlation spectroscopy; and comparing the denaturation signal of each target species in the presence of at least one test compound with a denaturation signal of the same target species in the absence of the at least one test compound under the same isothermal denaturing conditions.