Patent ID: 7022481
Filing Date: 2006-04-04
Classification: C12Q

Abstract:
1. A method for determining whether a molecule affects the function or activity of a glucan synthase pathway in a S. cerevisiae cell comprising: (a) contacting the cell with, or recombinantly expressing within the cell, the molecule; (b) determining whether the RNA expression or protein expression in said cell of at least one target polynucleotide sequence is changed in step (a) relative to the expression of said target polynucleotide sequence in the absence of the molecule, each said target polynucleotide sequence being operatively linked to a promoter native to a gene selected from the group consisting of YOL113W (SKM1), YNR066C, YLR121C (YPS3), YHR209W, YKL161C, YAR050W (FLO1), and YFR030W (MET10); and (c) determining that the molecule affects the function or activity of the glucan synthase pathway if the expression of said target polynucleotide is changed, or determining that the molecule does not affect the function or activity of the glucan synthase pathway if the expression of said target polynucleotide sequence is unchanged.