Patent ID: 7560535
Filing Date: 2009-07-14
Classification: C12P

Abstract:
1. A method of making a glycosylated antibody, the method comprising: incorporating an unnatural amino acid comprising an electrophilic reactive moiety into an antibody by expressing at least one recombinant nucleic acid that encodes at least one polypeptide chain of the antibody in a translation system, wherein the recombinant nucleic acid comprises at least one selector codon, and wherein the translation system comprises an orthogonal tRNA (OtRNA) and an orthogonal aminoacyl tRNA synthetase (ORS), wherein the ORS aminoacylates the OtRNA with the unnatural amino acid and the translation system incorporates the unnatural amino acid into the polypeptide chain in response to the selector codon, and, coupling a saccharide moiety, that is coupled to a nucleophilic moiety, to the electrophilic reactive moiety, wherein the electrophilic reactive moiety and the nucleophilic moiety react to chemically bond the saccharide moiety to the antibody, thereby glycosylating the antibody.