Patent ID: 7829350
Filing Date: 2010-11-09
Classification: A61K,B82Y,C07F,C07H,C07K,C12Q,G01N,Y10S,Y10T

Abstract:
1. A method for determining the presence of a target molecule in a sample, the method comprising obtaining a mixture of at least two different populations of conjugates that specifically bind to the target molecule to form an aggregate, wherein each conjugate in the first population comprises a nanoparticle comprising a magnetic metal oxide linked to a plurality of first binding moieties that bind to a first binding site on the target molecule, and wherein each conjugate in the second population comprises a nanoparticle comprising a magnetic metal oxide linked to a plurality of second binding moieties that bind to a second binding site on the target molecule different than the first binding site; contacting the mixture with a fluid sample under conditions that enable the first and second binding moieties to specifically bind to any target molecules in the sample and form an aggregate of conjugates; and determining the presence of one or more aggregates in the sample by measuring the spin-spin (T