Patent ID: 6316194
Filing Date: 2001-11-13
Classification: C12Q

Abstract:
A method for determining whether a test compound binds to a target RNA, the method comprising the steps of:(a) contacting the test compound with a pair of indicator molecules comprising an antimicrobial labelled with a donor group or an acceptor group and the target RNA labelled with a group that is acceptor or donor, respectively, to the label on said antimicrobial, wherein the pair of indicator molecules bind to each other in an orientation that permits the donor group to come into sufficient proximity to the acceptor group to permit fluorescent resonance energy transfer and/or quenching to take place; and(b) measuring fluorescence of the target RNA and the antimicrobial in the presence of the test compound and comparing this value to fluorescence of a standard, thereby determining whether said test compound binds said target RNA.