Patent ID: 8772033
Filing Date: 2014-07-08
Classification: A01H,C12N

Abstract:
1. A method of multiplying plant embryogenic tissue in a bioreactor comprising the steps of: (a) inoculating plant embryogenic tissue and multiplication medium into a bioreactor; (b) measuring a parameter that is a direct measure of, or correlated to, a concentration of the plant embryogenic tissue in the bioreactor; (c) determining a target value of the parameter; (d) taking the measurement obtained in step (b) and the target value determined in step (c) to calculate a flow rate of additional multiplication medium, wherein when the parameter is dry weight concentration, the flow rate of additional multiplication medium is calculated using the equation: wherein ΔB wherein when the parameter is sugar concentration, Brix or electrical capacitance, the flow rate of additional multiplication medium is calculated using the equation: wherein V wherein a proportional integral feedback controller is used to calculate the flow rate factor, and wherein the proportional integral feedback controller is a non-linear, discrete proportional integral controller; (e) delivering additional multiplication medium to the bioreactor based on the flow rate calculated in step (d); and (f) periodically repeating steps (b) to (e).