Patent ID: 8716442
Filing Date: 2014-05-06
Classification: A61B,C07K,C12N,C12Q,H01M,Y02E

Abstract:
1. A fusion polypeptide having both an electron mediator function and a substrate-oxidizing function, comprising: (a) an amino acid sequence of an extracellular secretion type cytochrome from a filamentous bacteria on the N-terminal side of the amino acid sequence of the fusion polypeptide; and (b) an amino acid sequence of a glucose dehydrogenase from a filamentous bacteria, in which flavin adenine dinucleotide is a coenzyme, on the C-terminal side of the amino acid sequence of the fusion polypeptide, wherein the extracellular secretion type cytochrome has the following properties: (1) capable of receiving an electron from a glucose dehydrogenase and/or giving an electron to an electron acceptor; (2) having a molecular weight of about 30 kDa as determined by SDS-PAGE; (3) having a characteristic absorption spectra at 427 nm, 531 nm, and 562 nm in a reduced form; (4) being cytochrome b562; (5) being a soluble protein; (6) having a signal sequence at the N-terminal; (7) having a first sequence “Gly-Xaa-Met” between positions 54-110 from a methionine of the N-terminal and having a proline nine amino acids away from the methionine of the first sequence; (8) having a second sequence “Asn-Xaa-Thr” between positions 101-160 from the methionine of the N-terminal and having a “Cys-Xaa-Xaa-Cys” seven amino acids away from the threonine of the second sequence; and (9) having a histidine between positions 158-214 from the methionine of the N-terminal sequence; and wherein the fusion polypeptide has a molecular weight of 115-124 kDa as determined by SDS-PAGE.