Patent ID: 6013484
Filing Date: 2000-01-11
Classification: A61K,C07K,C12N,G01N,Y10S

Abstract:
A process for making a monoclonal antibody that recognizes a protein or glycoprotein antigen of HIV-3 retrovirus (also known as HIV-1 subtype O virus), said retrovirus having the morphological and immunological characteristics of any of the retroviruses deposited in the European Collection of Animal Cell Cultures under V88060301, the process comprising:(a) infecting an animal with at least one antigen selected from a protein or a glycoprotein of HIV-3 to form antibody-producing cells;(b) fusing said antibody-producing cells with immortalized cells under hybridoma-forming conditions to form hybridomas;(c) selecting at least one hybridoma which secretes monoclonal antibodies which recognize the antigen as determined by western blot or by ELISA; and,(d) collecting monoclonal antibodies secreted by the hybridoma.