Patent ID: 7256030
Filing Date: 2007-08-14
Classification: C12N,C12Y,D21C,D21H

Abstract:
1. An isolated enzyme exhibiting beta-1,4-endoglucanase activity (EC 3.2.1.4) which (a) has a temperature optimum of 65° C. measured at a pH of 7.5 and (b)(i) has an amino acid sequence that is at least 95% identical to amino acids 1-456 or 1-617 of SEQ ID NO: 2 wherein identity is determined by GAP provided in the GCG program package using a GAP creation penalty of 3.0 and GAP extension penalty of 0.1or (ii) is encoded by a DNA sequence that hybridizes to nucleotides 76-1455 of SEQ ID NO: 1 under high stringency conditions, wherein the high stringency conditions are defined as hybridization in 5xSSC at 45° C. and washing in 2xSSC at 70° C.