Patent ID: 8993256
Filing Date: 2015-03-31
Classification: C12Q,G01N

Abstract:
1. An isolated polynucleotide encoding a fusion polypeptide comprising: (i) a polypeptide of interest, (ii) a protease, (iii) a protease cleavable linker, and (iv) a tag moiety selected from the group consisting of a fluorescent moiety, a fluorescent moiety that undergoes FRET, a fragment of a fluorescent protein a bioluminescent moiety, a bioluminescent moiety that undergoes BRET, a fragment of a bioluminescent moiety, a peptide or protein that affects signal transduction or the cell cycle, a marker enzyme, a marker enzyme fragment, and a protein toxin, wherein each of (i)-(iv) are operably linked, and wherein the protease can be inhibited by contacting with a protease inhibitor.