Patent ID: 7915201
Filing Date: 2011-03-29
Classification: C07H,C12N,C12P,C40B

Abstract:
1. A method for synthesising a bifunctional complex comprising an encoded molecule and an identifier polynucleotide identifying the chemical entities having participated in the synthesis of the encoded molecule, said method comprising the steps of providing a) at least one template comprising one or more codons capable of hybridising to an anti-codon, and b) a plurality of building blocks each comprising an anti-codon associated with one or more chemical entities, and hybridising the anti-codon of one or more of the provided building blocks to the template, covalently linking each of said anti-codons to at least one other anti-codon and optionally covalently linking the at least one template with the anti-codon of at least one building block, thereby generating an identifier polynucleotide capable of identifying chemical entities having participated in the synthesis of the encoded molecule, separating the template from one or more of the anti-codons hybridised thereto, thereby generating an at least partly single stranded identifier polynucleotide associated with a plurality of chemical entities, wherein at least part of the template is covalently linked to the identifier polynucleotide in the form of covalently linked anti-codons, and/or wherein chemical entities of different building blocks are reacted under conditions wherein the identifier polynucleotide is at least essentially single stranded, generating a bifunctional complex comprising an encoded molecule and an identifier polynucleotide identifying the chemical entities having participated in the synthesis of the encoded molecule, wherein said encoded molecule is generated by reacting at least two of said plurality of chemical entities associated with the identifier polynucleotide, wherein said at least two chemical entities are provided by separate building blocks.