Patent ID: 6022723
Filing Date: 2000-02-08
Classification: C12N,C12Y,Y10S

Abstract:
A DNA construct comprising a polynucleotide sequence encoding an enzyme exhibiting .beta.-(1-6)-glucanase activity, wherein the polynucleotide comprises(a) the polynucleotide sequence of SEQ ID NO:3, or a fragment thereof encoding an enzymatically active polypeptide;(b) a polynucleotide encoding the amino acid sequence of SEQ ID NO:4; or(c) a polynucleotide which hybridizes to (a)-(b) under the following conditions: prehybridization for 1 h at about 40.degree. C. in a solution of 5.times.SSC, 5.times.Denhart's, 50 mM sodium phosphate, pH 6.8, and 50 .mu.g denatured sonicated calf thymus DNA, followed by hybridization in the same solution for 18 h at about 40.degree. C., followed by washing three times in 2.times.SSC, 0.2% SDS at 40.degree. C. for 30 min, wherein the polynucleotide encodes an enzyme exhibiting .beta.-(1-6)-glucanase activity.