Patent ID: 7786282
Filing Date: 2010-08-31
Classification: A61K,A61P,C07K

Abstract:
1. A nucleic acid molecule encoding a chimeric TNFα ligand polypeptide having a CD154 Domain III and a TNFα Domain IV, wherein: (a) the Domain III lacks a metalloproteinase cleavage site present in CD154; and, (b) the Domain IV binds to a TNF receptor; wherein the encoded chimeric polypeptide is more resistant to cell membrane cleavage into soluble TNFα than are human pro-TNFα and human pro-TNFα lacking the TACE mmp recognition site spanning Val77 and Pro88 of human TNFα, when expressed in HeLa, 293, A549, COLO205, HCT15, BT20 or HT1080 cells.