Patent ID: 8846049
Filing Date: 2014-09-30
Classification: A61K,A61P,C07H,C12N,Y02A

Abstract:
1. A method of conjugating a saccharide to a protein carrier using carbodiimide condensation chemistry, wherein the saccharide has been derivatised to comprise either amino or carboxyl groups, and wherein the protein carrier comprises, or has been derivatised to comprise, both amino and carboxyl groups, said method comprising the steps of: a) mixing the saccharide and aliquot of carbodiimide required to perform the conjugation, and b) adding the aliquot of protein carrier required over a period of 5 minutes to 6 hours; wherein the initial ratio of protein carrier to saccharide is 4:1 to 1:1 (w:w); and wherein the saccharide is a bacterial capsular saccharide derived from