Patent ID: 6537823
Filing Date: 2003-03-25
Classification: G01N,Y10T

Abstract:
The method for the detection of bromine in a biological sample, wherein the biological sample is selected from the group consisting essentially of urine, serum, whole blood, cerebral spinal fluid, gastric fluid, hair homogenates, sweat extracts or saliva, consisting essentially of;(A) preparing a dry chemistry test means by successively impregnating a solid, carrier matrix with reagent solutions consisting essentially of an indicator and a buffer, wherein the indicator is selected from the group consisting of 1,2,3,4-tetrahydrobenzo(h)quinolin-3-ol, 1,2,3,4-tetrahydrobenzo(h)quinolone, 1,3-phenylenediamine, 1,2,3,4-tetrahydroquinoline, 1,2,3,4-tetrahydroisquinoline hydrochloride, 7,8-benzoquinoline, [1,2,3,4-tetrahydro-3-isoquinolinecarboxylicacid hydrochloride,] 1,2,3,4-tetrahydro-1-napthylamine hydrochloride, napthylamine, 2,2â€²-Azino-di-(3-ethylbenzthiazolinesulfonic acid), 2,2â€²-Azino-di-(3-ethylbenzthiazolinesulfonic acid) diammonium salt, 3,3â€²-diaminobenzidine, o-dianisidine, dimethoxybenzidine, 0-phenylenediamine, 3-amino-9-ethylcarbazole, 3,3â€²-5,5â€²-tetramethylbenzidine, dimethoxybenzidine, 8-hydroxyquinoline, m-phenylenediamine, 3-dimethylaminobenzoic acid, 5-aminosalicylic acid, 4-chloro-1-napthol, diazotizable amine, sulfanilic acid, arsanilic acid, sulfanilamide, aminobenzoic acid or 4-aminoantipyrine in combination with one of the following compounds; p-hydroxybenzene sulfonate, p-hydroxybenzoic acid, n-ethyl-n-(2-hydroxy-3-sulfopropyl)-m-toluidine, n-ethyl-n-sulfopropyl-m-toluidine, 2-hydroxy-3,5-dichlorobenzenesulfonic acid, 3-hydroxy-2,4,6-triiodobenzoic acid, and 3-hydroxy-2,4,6-tribromobenzoic acid (B) drying the impregnated solid carrier matrix; (C) dipping said chemistry test means into the biological sample; and (D) observing a detectable response in the form of a color developed in the presence or absence of bromine, wherein the color is observable to the human eye or visible light spectrum.