Patent ID: 9068224
Filing Date: 2015-06-30
Classification: C12P,C12Q

Abstract:
1. A method of determining the clonal expansion of B cells in response of an individual to a vaccine, the method comprising: obtaining a cell sample comprising B cells from said individual in a short defined time period following immunization with said vaccine; dividing said cell sample comprising B cells, or genomic DNA derived therefrom, into at least two distinct pools; amplifying genomic DNA sequences at an immunoglobulin heavy chain locus in said at least two distinct pools with a primer set that amplifies at least 50% of the known rearrangements at the locus; sequencing at least 10 comparing sequences from said amplified genomic DNA to detect the presence of sequences that are coincident in said at least two distinct pools of nucleic acid; wherein the presence of coincident sequences is indicative of clonal expansion and responsiveness to said vaccine.