Patent ID: 8263377
Filing Date: 2012-09-11
Classification: C12Q,G01N

Abstract:
1. A method for characterizing a kinase inhibitor candidate using Surface Plasmon Resonance (SPR), comprising: a) providing a kinase inhibitor candidate; b) providing a compound of the formula: wherein SG is a signal inducing group; L is a linker; KBM is a kinase binding molecule; m is an integer from 1 to 5; and n is an integer from 0 to 5; c) providing an immobilized kinase SPR array; d) contacting the kinase inhibitor candidate and the compound with the immobilized kinase SPR array; e) measuring the SPR signal of one or both the compound and the kinase inhibitor candidate with the immobilized kinase SPR array; and f) monitoring a change in the SPR signal upon binding or release of the compound or the kinase inhibitor candidate with the immobilized kinase SPR array, thereby characterizing the kinase inhibitor.