Patent ID: 7875433
Filing Date: 2011-01-25
Classification: G01N,Y10S

Abstract:
1. A method of determining a relative amount of at least two analytes of interest in a fluid test sample, comprising: a) providing a solid phase apparatus comprising an application point and at least two sample capture zones, the first sample capture zone having a first sample capture reagent adsorbed thereon and the second sample capture zone having a second sample capture reagent adsorbed thereon; b) providing analyte binding particles, wherein the analyte binding particles comprise either: i) a population of first analyte binding particles and a population of second analyte binding particles, wherein the first analyte binding particles are coated with a first analyte binding agent and the second analyte binding particles are coated with a second analyte binding agent; or ii) a population of analyte binding particles, wherein the analyte binding particles are coated with a first analyte binding agent and a second analyte binding agent; c) combining the test sample, the analyte binding particles, and the solid phase apparatus, thereby producing contacted analyte binding particles; d) maintaining the solid phase apparatus under conditions which allow contacted analyte binding particles to bind to the first sample capture reagent in the first sample capture zone, and allow contacted second analyte binding particles to bind to the second sample capture reagent in the second sample capture zone; and e) determining the ratio of the amount of contacted first analyte binding particles in the first sample capture zone to the amount of contacted second analyte binding particles in the second capture zone, wherein the ratio is equal to the relative amounts of the analytes of interest in the fluid sample.