Patent ID: 6387633
Filing Date: 2002-05-14
Classification: C12N

Abstract:
A method for identifying an unstable gene that is involved in genomic instability in cells, comprising the steps of:(a) transfecting the cells with a vector expressing human mts-1 or a functional analog thereof; (b) selecting for the transfected cells that express human mts-1 or the functional analog; (c) subcloning the cells resulting from step (b); (d) comparing the phenotypes of the subclones resulting from step (c); (e) determining a correlation coefficient for each gene in a gene panel by comparing the gene dosage of each gene in the gene panel with the gene dosage of a marker gene in genomic DNA isolated from the subclones; and (f) identifying a gene in the gene panel as an unstable gene if the phenotypes of the subclones demonstrate diversity and the correlation coefficient of the gene in the gene panel is less than 0.3.