Patent ID: 8183015
Filing Date: 2012-05-22
Classification: C12N,C12Q

Abstract:
1. A method for verifying the efficiency of sample preparation of test sample nucleic acids and the performance of nucleic acid amplification and/or detection practiced on a test sample after its preparation, said method comprising: (i) providing a universal internal control reagent comprising viable (ii) mixing said universal internal control reagent and said test sample; (iii) submitting said test sample mixed with said universal internal control reagent to a sample preparation procedure in order to release both said nucleic acid from said test sample and said IC nucleic acid target sequence from said universal internal control reagent; and (iv) submitting a product from said sample preparation procedure to amplification and/or detection for the amplification and/or detection of both said IC nucleic acid target sequence and said nucleic acid of the test sample, wherein detection of said IC nucleic acid target sequence is indicative of both efficient sample preparation and performance of nucleic acid amplification and/or detection.