Patent ID: 9063147
Filing Date: 2015-06-23
Classification: A23L,A61P,C07K,C12N,G01N

Abstract:
1. A method for determining an amount of ferritin iron from a legume in a sample comprising the steps of: (a) first, providing a sample comprising a legume; (b) second, determining the total amount of iron in the sample; (c) third, preparing an aqueous soluble extract of the sample to make a soluble sample extract; (d) fourth, determining the total amount of iron in the soluble sample extract; (e) fifth, determining the amount of legume ferritin protein in the soluble sample extract using an automated quantitative immunological analysis, wherein the automated quantitative immunological analysis is capillary electrophoresis, and wherein the automated quantitative immunological analysis is conducted utilizing an antibody specific to the legume ferritin protein; and (f) sixth, determining the amount of ferritin iron in the sample using the amount of total iron in the soluble sample extract divided by the amount of the legume ferritin protein in the soluble sample extract, multiplied by 0.75, multiplied by the total amount of iron in the sample, thereby determining the amount of ferritin iron from the legume in the sample.