Patent ID: 6825021
Filing Date: 2004-11-30
Classification: C12N

Abstract:
An isolated polynucleotide that encodes a dual specificity phosphatase-15 (DSP-15) substrate trapping mutant polypeptide in which a DSP-15 polypeptide comprising an amino acid sequence set forth in SEQ ID NO:2 has a substitution of an amino acid residue selected from the group consisting of (i) the aspartic acid residue at position 382 of SEQ ID NO:2 and (ii) the cysteine residue at position 413 of SEQ ID NO:2, wherein the DSP-15 substrate trapping mutant polypeptide retains the ability to bind a DSP-15 substrate, and wherein the ability of the DSP-15 substrate trapping mutant polypeptide to dephosphorylate the DSP-15 substrate is reduced relative to the DSP-15 polypeptide.