Patent ID: 9045522
Filing Date: 2015-06-02
Classification: C07H,C12P,C12Q

Abstract:
1. A method for regenerating a 3′ hydroxyl group, the method comprising: using a mixture comprising at least one reversibly modified oligonucleotide having a non-hydroxyl group 3′ end which can be converted into a 3′ hydroxyl group, wherein the oligonucleotide has a carboxylic acid ester group at its 3′ end; exposing the mixture to a first chemical and a first range of temperature, wherein the first chemical and the first range of temperatures regenerate the 3′ hydroxyl group, wherein the first chemical is an amine; wherein the amine is selected from the group consisting of methylamine, ethylenediamine, and triethylamine, and regenerating the 3′ hydroxyl group of the at least one reversibly modified oligonucleotide having a non-hydroxyl 3′ end.