Patent ID: 6120676
Filing Date: 2000-09-19
Classification: A61B,C12Q,G01N

Abstract:
A method of determining a concentration of an analyte in a sample, the method comprising steps of:contacting the sample with an electrochemical sensor comprising a working electrode, a counter electrode, and a measurement zone bounded on at least two sides by the working electrode and counter electrode, wherein the measurement zone is sized to contain less than about 1 .mu.L of sample;holding the sample within the measurement zone in a non-flowing manner;applying a potential between the working and counter electrodes to electrolyze a portion of the analyte in the sample in the measurement zone;measuring current generated by the electrochemical sensor at a plurality of times while portion of the analyte is being electrolyzed; anddetermining, by coulometry, a concentration of the analyte in the sample using the measured currents.