Patent ID: 6482618
Filing Date: 2002-11-19
Classification: A61K,A61P,C12N

Abstract:
A nucleic acid construct that comprises in a 5â€² to 3â€² direction:(i) at least one activation sequence comprised of at least one sequence that binds a transcription factor protein and at least one promoter sequence that comprises (a) at least one promoter and at least one gene coding for a first fusion protein that comprises an activation domain of a transcription factor protein and a coupling substance protein, and (b) at least one promoter and at least one gene coding for a second fusion protein that comprises a DNA-binding protein and a coupling substance protein, and (c) at least one activation sequence that comprises a site for the DNA-binding protein, wherein in presence of a coupling substance the fusion proteins are linked to each other form a functional transcription factor protein for activating the activation sequence; and (ii) at least one structural gene, and (iii) at least one structural gene that encodes the transcription factor protein, wherein the activation of the activation sequence activates the expression of a structural gene and the expression of the transcription factor protein.