Patent ID: 8849575
Filing Date: 2014-09-30
Classification: G16B,G16C

Abstract:
1. A method of defining a library of biological molecules, the method comprising: (a) receiving an original set of data points representing activity and sequence of multiple biological molecules in a training set, wherein the activity of the multiple biological molecules was obtained in vitro; (b) constructing a bootstrap set of data points by computationally selecting said bootstrap set of data points from the original set of data points; (c) generating a sequence activity model from the bootstrap set, (d) repeating (b) and (c) multiple times to generate multiple models, each of the multiple models providing one of multiple values for each coefficient; (e) for each coefficient, determining (i) an average or mean value of the multiple values and (ii) a statistical indication of the distribution of the multiple values; and (f) toggling particular residues or codons based on their respective values of (i) and (ii) determined in (e) to thereby define the library of biological molecules, wherein operations (a)-(f) are performed by executing instructions on a computer system programmed to perform said operations.