Patent ID: 7645605
Filing Date: 2010-01-12
Classification: C12N,C12P

Abstract:
1. An isolated protein described in the following (a), or (b): (a) an isolated protein, which comprises an amino acid sequence wherein at least one amino acid residue selected from among the phenylalanine residue at position 24, the isoleucine residue at position 88, the glutamic acid residue at position 92, the glutamic acid residue at position 93, the histidine residue at position 96, the glutamic acid residue at position 103, the asparagine residue at position 167, and the tyrosine residue at position 225, is substituted with another amino acid residue in the amino acid sequence of SEQ ID NO: 2 of the β subunit of a wild-type nitrile hydratase, and which has a heat-resistant nitrile hydratase activity; or (b) an isolated protein, which comprises an amino acid sequence wherein the asparagine residue at position 167 is substituted with another amino acid sequence in the amino acid sequence of SEQ ID NO: 2 of the β subunit of a wild-type nitrile hydratase, and which has a heat-resistant nitrile hydratase activity.