Patent ID: 6171823
Filing Date: 2001-01-09
Classification: C07K,C12N,Y10S

Abstract:
A method for producing an extracellular protein in a bacterium, the method comprisinga) providing a recombinant vector including a DNA construct encoding a fusion protein, wherein said fusion protein comprises an N-terminal prepropeptide, but not a C-terminal propeptide, of Achromobacter lyticus protease I, corresponding to residues 1-205 of SEQ ID NO:27, fused to a polypeptide sequence comprising a desired protein;(b) transforming cells of a bacterium having an inner and outer cell membrane with the recombinant vector of step (a);(c) culturing the transformed cells of step (b) under conditions permitting expression of the fusion protein and(d) recovering the fusion protein, the desired protein, or both from the extracellular medium.