Patent ID: 9073972
Filing Date: 2015-07-07
Classification: C07K

Abstract:
1. A method of separating cellular components, the method comprising: a) contacting a guest compound-bound reactive compound with cells; b) binding the guest compound-bound reactive compound to a cellular component of the cell; c) lysing the cells; d) adding a host compound-bound solid phase to a solution comprising the lysates of the cells to prepare a mixture; e) separating binding pairs of the guest compound bound to cellular components and the host compound bound to the solid phase from the mixture, and purifying the binding pairs; and f) separating the cellular components from the binding pairs, wherein the host compound-bound solid phase is obtained through a covalent bond between a solid phase and a host compound represented by Formula 1 below, wherein n is an integer in the range of 6 to 10, X is one selected from the group consisting of O, S, and NH, A wherein the guest compound-bound reactive compound is a compound selected from a group consisting of Formulae 12 and 13 below: