Patent ID: 6632602
Filing Date: 2003-10-14
Classification: C12N

Abstract:
A method for modifying sugar sensitivity in a transgenic plant cell by increasing the level of a hexokinase protein in said transgenic plant cell, said method comprising expressing in said transgenic plant cell a hexokinase nucleic acid sequence that specifically hybridizes under highly stringent conditions to the complement of the sequence set forth in SEQ ID NO.: 3 or SEQ ID NO.: 4, wherein said highly stringent conditions comprisea) hybridization at 65Â° C. and 50% formamide, b) a first wash at 65Â° C., 2Ã—SSC, and 1% SDS, and c) a second wash at 65Â° C., 1Ã—SSC, and 0.1% SDS; and said nucleic acid encodes a hexokinase protein comprising a sugar binding domain, wherein expression of said hexokinase nucleic acid sequence increases the level of said hexokinase protein in said transgenic plant cell.