Patent ID: 6100079
Filing Date: 2000-08-08
Classification: C12N,C12Q,G01N,Y10S

Abstract:
A method of purifying and separating biopolymers from a homogenized biological milieu using a pipette tip and a container, comprising the steps of:depositing the biological milieu in the container without immobilization of the biopolymers;adding first magnetic particles to the biological milieu to complex with the biopolymers;removing the biological milieu from the container with the pipette tip;applying a magnetic field to the pipette tip, thereby attracting the biopolymer/magnetic particle complex to the inner surface of the pipette tip for separation from the biological milieu;removing the magnetic field from the pipette tip;dissociating and separating the magnetic particles from the biopolymers;binding second magnetic particles to the biopolymers; andapplying the magnetic field to the pipette tip, thereby attracting the bound biopolymers to the inner surface of the pipette tip to allow separation from impurities.