Patent ID: 6306592
Filing Date: 2001-10-23
Classification: C12Q,Y10T

Abstract:
A method of detecting and quantifying nucleic acid molecules within a population I of nucleic acid molecules of identical, similar or different sequence, comprising a) converting single-stranded or duplex ribonucleic acid (RNA) molecules or single-stranded deoxyribonucleic acid (DNA) molecules contained in population I into a population II of duplex DNA molecules, b) denaturating and subsequently renaturating the population II of duplex DNA molecules thereby forming a mixture of homo-duplex and heteroduplex DNA molecules, c) reacting chemically with a carbodiimide compound the heteroduplexes formed in the population II where unpaired or incorrectly paired nucleotides are present, d) detecting and quantifying the resulting carbodiimide reaction products, and performing a chromatographic purification method between each of steps a to d.