Patent ID: 9040774
Filing Date: 2015-05-26
Classification: C12N,Y02A

Abstract:
1. A recombinant DNA construct comprising a promoter operable in a plant cell, operably linked to DNA encoding a single-stranded cleavage blocker RNA that binds in vivo to a target RNA transcript in said plant cell, at an miRNA recognition site for an endogenous mature miRNA, and forms, through complementary base-pairing, a hybridized segment of between 19 to 24 nucleotides in length at said miRNA recognition site in said target RNA transcript, wherein said hybridized segment comprises: at least one nucleotide in said single-stranded cleavage blocker RNA that does not match through complementary base-pairing with said miRNA recognition site at positions corresponding to positions 10 or 11 of said endogenous mature miRNA; and wherein the single-stranded cleavage blocker RNA interferes with the binding of said endogenous mature miRNA to said target RNA transcript at said miRNA recognition site.