Patent ID: 7939323
Filing Date: 2011-05-10
Classification: C12N

Abstract:
1. A method for the simultaneous isolation of four cell types from one umbilical cord sample comprising the subsequent steps of: a) isolating endothelial cells by perfusing a vein from a section of said umbilical cord with a solution comprising thermolysin for a period of time to allow detachment of said endothelial cells from said vein; b) treating the section of said umbilical cord of step a) with a solution of trypsin-EDTA for a time sufficient to allow detachment and isolation of epithelial cells; c) mechanically removing smooth muscle cells and fibroblast-like cells from the section of said umbilical cord of step b) substantially devoid of endothelial and epithelial cells, followed by a culture period of sufficient time to allow separation and isolation of smooth muscle and fibroblast-like cells from laminae; and d) harvesting the isolated cells of steps a) to c).