Patent ID: 6607911
Filing Date: 2003-08-19
Classification: C12Q

Abstract:
An isolated control DNA construct comprising a vector portion for expression in a cell and a target nucleic acid comprising two or more nucleic acid fragments wherein each fragment specifies a component associated with at least one of a disease state, an environmental condition, or a biological organism, wherein said component is different from a component specified by any other fragment present elsewhere in said construct, and wherein the 5â€²-most fragment is linked to said vector portion via a restriction site not present elsewhere in said construct, and wherein the 3â€²-most fragment is linked to said vector portion via a restriction site not present elsewhere in said construct, and further wherein each said fragment is flanked by a restriction site not present elsewhere in said construct, wherein each of said fragments comprise at least one exon of a gene, wherein said exon is a cystic fibrosis transmembrane conductance regulator (CFTR) exon, further wherein said CFTR exon is selected from the group consisting of exon 10 and exon 11, and wherein said restriction site is selected from the group consisting of a BssH II site, a Csp45 I site, a Age I site, and a Nco I site.