Patent ID: 9134304
Filing Date: 2015-09-15
Classification: C07K,C12N,G01N

Abstract:
1. An X-display complex comprising: (a) a nucleic acid molecule comprising a polypeptide-encoding sequence, wherein the nucleic acid molecule is covalently bound to a first high affinity ligand; (b) a polypeptide encoded by the nucleic acid molecule, wherein the polypeptide is covalently linked at the C-terminal residue through a peptide acceptor to a second high affinity ligand, and wherein the peptide acceptor is selected from the group consisting of puromycin, pyrazolopyrimidine, phenylalanyl-adenosine, tyrosyl adenosine, alanyl adenosine, phenylalanyl 3′ deoxy 3′ amino adenosine, alanyl 3′ deoxy 3′ amino adenosine, and tyrosyl 3′ deoxy 3′ amino adenosine; and (c) a single multimeric ligand acceptor protein, wherein the single multimeric ligand acceptor protein is bound noncovalently and directly to both the first and second high affinity ligand at distinct ligand binding sites on the ligand acceptor protein, thereby noncovalently linking the nucleic acid molecule and the polypeptide.