Patent ID: 6075134
Filing Date: 2000-06-13
Classification: A61K,A61P,B82Y,C07H

Abstract:
A method for making a functionalized glycoconjugate, said method comprising the steps of:a) contacting a cell with a first monosaccharide comprising a first functional group; andb) incubating said cell under conditions whereby said cell (i) internalizes said first monosaccharide, (ii) biochemically processes said first monosaccharide into a second monosaccharide comprising a second functional group, (iii) conjugates said second monosaccharide to a carrier to form a glycoconjugate comprising a third functional group, and (iv) extracellularly expresses said glycoconjugate to form an extracellular glycoconjugate comprising a fourth, selectively reactive, functional group;wherein said first functional group is N-linked in said first monosaccharide,said first monosaccharide comprises a ManLev,said cell is a eukaryotic cell, andsaid fourth functional group is a ketone.