Patent ID: 8586099
Filing Date: 2013-11-19
Classification: A61F,A61L,A61P

Abstract:
1. A method for preparing a bone graft substitute from bovine bone, the method comprising the steps of: (a) boiling bovine bone, from which blood components have been removed, in deionized water to remove lipids and proteins; (b) drying the boiled bone; (c) grinding the dried bone to a powder; (d) immersing and shaking the ground bone powder in an organic solvent; (e) drying the bone powder to remove the organic solvent; (f) treating the dried bone powder, from which the solvent has been removed, with a solution comprising 4-20% (w/v) sodium hypochlorite to inactivate prion protein; (g) removing the sodium hypochlorite solution from the bone powder; (h) drying the resulting bone powder from which the sodium hypochlorite has been removed; and (i) thermally treating the dried bone powder of step (h) at 600-1000° C. for 1-6 hours to completely remove lipids and proteins.