Patent ID: 6294341
Filing Date: 2001-09-25
Classification: G01N,Y10S

Abstract:
An immunoassay for detecting the presence of GST-C bound to Trx-N comprising:adsorbing Trx-N variant protein (SEQ ID NO:6) on a surface of a cell culture vessel;adding GST-C (SEQ ID NO:9);removing GST-C which is not bound to Trx-N;adding a primary antibody which binds to GST;removing the primary antibody which is not bound to GST-C;adding a secondary antibody which binds to the primary antibody;removing the secondary antibody which is not bound to the primary antibody;adding peroxide and o-phenylenediamine; anddetecting the color development of o-phenylenediamine oxidized by peroxidase which is chemically bound to the secondary antibody.