Patent ID: 8932620
Filing Date: 2015-01-13
Classification: A61K,A61L,C12N

Abstract:
1. A method of making a biologically active three-dimensional scaffold comprising at least one of fibers or droplets, capable of supporting growth and differentiation of a cell, the method comprising: providing an unfractionated mixture of extracellular matrix proteins extracted from a basement membrane; freezing the extracted unfractionated mixture of extracellular matrix proteins at a temperature of at least −60° C. and freeze drying under a negative pressure to provide a lyophilized extracted unfractionated mixture of extracellular matrix proteins; combining the lyophilized extracted unfractionated extracellular matrix proteins with an aqueous or non-aqueous solvent to form a solution and stirring the solution at about 50° C.; and electroprocessing the solution comprising the lyophilized extracted unfractionated extracellular matrix proteins to form the biologically active three-dimensional scaffold comprising at least one of fibers or droplets and capable of supporting growth and differentiation of a cell, and wherein the fibers or droplets have an average diameter of between about 30 nm and about 1 μm.