Patent ID: 6713662
Filing Date: 2004-03-30
Classification: A01K,A23C,A23J,A61P,C07K,C12N

Abstract:
A transgenic nonhuman mammal having a transgene comprising:a mammary-gland specific promoter; a mammary-gland specific enhancer a secretory DNA segment encoding a signal peptide functional in mammary secretory cells of the transgenic nonhuman mammal; and a recombinant DNA segment encoding an exogenous procollagen polypeptide operably linked to the secretory DNA segment to form a secretory-recombinant DNA segment, the secretory-recombinant DNA segment being operably linked to the promoter and to the enhancer; wherein the transgene, in an adult form of the nonhuman mammal or a female descendant of the nonhuman mammal, expresses the secretory-recombinant DNA segment in the mammary secretory cells to produce a form of the exogenous procollagen polypeptide that is processed and secreted by the mammary secretory cells into milk as exogenous procollagen or collagen in a detectable amount; provided the recombinant DNA segment comprises a genomic DNA segment from the &agr;1(I) collagen gene and is without nucleotides 1-114 from the first exon of the gene, and/or without a segment from the first intron of the gene.