Patent ID: 8922638
Filing Date: 2014-12-30
Classification: G01N,G02B

Abstract:
1. A method of imaging a sample structure with a light microscope having a resolution limit, the method comprising the steps of: preparing the sample structure with markers configured to be brought into a state in which the markers are configured to be imaged by the light microscope; generating an active marker subset by bringing a portion of the markers into the state in which the markers are configured to be imaged by the light microscope; checking for presence of a predetermined activation state in which markers in the active marker subset have an average distance from one another greater than a minimum distance predetermined by the resolution limit of the light microscope by generating at least two test single frames at an interval, the at least two test single frames being captured by a plurality of sensor elements generating respective image signals, and comparing the at least two test single frames to one another in order to detect changes of corresponding image signals between the at least two test single frames, wherein the presence of the predetermined activation state is found if the detected changes of the image signals collectively exceed a predetermined quantity; and imaging the sample structure onto the plurality of sensor elements when the predetermined activation state is present, wherein the sensor elements generate respective image signals resulting in a single frame of a sample structure image.