Patent ID: 8492112
Filing Date: 2013-07-23
Classification: C12N,G01N

Abstract:
1. A method for manufacturing a microtissue with a core-shell structure for inducing the growth of a hair follicle for mass generation, comprising the steps of: (A) providing an EVAL (ethylene-co-vinyl alcohol) membrane; (B) simultaneously seeding a plurality of dermal papilla (DP) cells and keratinocytes on the EVAL membrane surface with a predetermined ratio and a predetermined cell density, wherein the predetermined ratio of dermal papilla cells to keratinocytes is from 1:5 to 5:1, and the predetermined cell density of dermal papilla cells and keratinocytes is from 1.39×104cells/cm2 to 20.0×104 cells/cm (C) co-culturing DP cells and keratinocytes to form the microtissue with the core-shell structure, wherein the core-shell structure comprises DP cells in the center and keratinocytes on the outer surface, wherein said keratinocytes are adult keratinocytes, the microtissue induces hair follicle differentiation of keratinocytes; and (D) in 3 days, collecting 1050 to 2050 microtissues, wherein the microtissues have a high cell viability above 90%.