Patent ID: 6300063
Filing Date: 2001-10-09
Classification: B01J,C07H,C12Q,C40B

Abstract:
A method of identifying whether a target nucleic acid sequence includes a polymorphism, comprising:hybridizing said target nucleic acid sequence to an array comprising at least one detection block of probes, said detection block including a first group of probes that are complementary to said target nucleic acid sequence except that the first group of probes includes a plurality of monosubstitutions of positions in said sequence that are within n bases of a base in said sequence that is complementary to said polymorphism, wherein n is from 0 to 5, and a second and third group of probes complementary to marker-specific regions upstream and downstream of the target nucleic acid sequence, wherein the third group of probes differs from the second set of probes at single bases corresponding to known mismatch positions; anddetermining hybridization intensities of the target nucleic acid and the marker-specific regions to identify said polymorphism.