Patent ID: 6733747
Filing Date: 2004-05-11
Classification: A61F,A61K,A61L,C07K,C12N

Abstract:
A prosthetic graft for containment of blood flow in vivo, comprising:(a) a porous prosthetic implant for containing blood in vivo, said prosthetic implant having an outer surface that is not in contact with blood flow in vivo and an inner surface that is in contact with blood flow in vivo, said inner surface defining an interior space for containment of blood flow; and, (b) naturally adherent cells adhered to the outer surface of said porous prosthetic implant, wherein said naturally adherent cells are not seeded on the inner surface of said porous prosthetic implant, wherein said naturally adherent cells are transfected with at least one recombinant nucleic acid molecule operatively linked to a transcription control sequence, said recombinant nucleic add molecule encoding a protein, wherein said protein is expressed by said cells ex vivo, wherein at least a portion of said protein, upon secretion from said cells, perfuses through pores of said prosthetic implant to said inner surface of said prosthetic implant, and wherein said protein is selected from the group consisting of a protein that enhances angiogenesis, a protein that inhibits thrombosis, a protein that causes thrombolysis, a protein that inhibits smooth muscle migration or proliferation and a vasodilator protein.