Patent ID: 6057135
Filing Date: 2000-05-02
Classification: C12N,C12P

Abstract:
A method of manufacturing D-tagatose from cheese whey, milk, or cheese whey and milk, comprising the following sequential steps:subjecting the cheese whey, milk, or cheese whey and milk to ultrafiltration to make a lactose permeate;hydrolyzing the lactose permeate to make a lactose hydrolysate comprised of D-galactose and glucose;subjecting the lactose hydrolysate to fermentation conditions whereby the glucose is selectively fermented to ethanol;separating the D-galactose from the ethanol, making a solution having a concentration of from about 10% to about 60% by weight D-galactose;subjecting the solution of D-galactose to enzymatic isomerization with L-arabinose isomerase at an isomerization pH from about 5.5 to about 7.0 and a temperature from about 50.degree. C. to about 70.degree. C.; andwherein the yield of D-tagatose is from about 20% to about 45% by weight based on D-galactose.