Patent ID: 8399633
Filing Date: 2013-03-19
Classification: B01D,C07K

Abstract:
1. A method of removing lipopolysaccharide from a recombinantly produced protein comprising a lipopolysaccharide-protein complex of lipopolysaccharide and protein comprising the steps of: (a) loading the recombinantly produced protein comprising the complex on to an ion exchange chromatographic resin; (b) washing the resin with an alkanediol wherein the lipopolysaccharide is separated from the complex and the protein is affixed to the resin; and (c) eluting the protein affixed to the resin to form an eluent such that the ratio of the protein to the lipopolysaccharide in the eluent is increased relative to the ratio of the protein to the lipopolysaccharide in the complex present in the recombinantly produced protein loaded on to the chromatographic resin in step (a).