Patent ID: 8323895
Filing Date: 2012-12-04
Classification: C07H,C12Q

Abstract:
1. A method for selectively amplifying a target nucleic acid sequence from a DNA or a mixture of nucleic acids, which comprises amplifying the target nucleic acid sequence by performing at least two cycles of primer annealing, primer extending and denaturing using a primer set comprising a pair of dual specificity oligonucleotides with enhanced annealing specificity; wherein the dual specificity oligonucleotide is represented by the following general formula: wherein, Xp represents a 5′-high T Yq represents a separation portion comprising at least three contiguous universal bases; Zr represents a 3′-low T p, q and r represent the number of nucleotides; p represents an integer of at least 15; q represents an integer of at least 3; r represents an integer of at least 3; X, Y, and Z are deoxyribonucleotide or ribonucleotide; the T the separation portion has the lowest T the 5′-high T the separation portion forms a non base-pairing that does not serve as a hybridizing site and not interact with the target nucleic acid sequence for base pairing, under conditions that the 5′-high T wherein annealing in the amplification reaction is performed under conditions that annealing solely by the 3′-low T wherein a first cycle and a second cycle of the amplification reaction involve annealing of both the 5′-high T