Patent ID: 6440671
Filing Date: 2002-08-27
Classification: C12Q,Y10T

Abstract:
A method for detecting disease associated alleles in patient genetic material comprising:a) immobilizing a first group of oligonucleotide molecules having a known length on a predetermined position on a substrate, said oligonucleotide molecules synthesized to complement base sequences of the disease associated alleles; b) contacting the patient genetic material with said first group of oligonucleotides to form duplexes; c) contacting the duplexes with a second group of oligonucleotide molecules, said second group of oligonucleotide molecules to noncovalently to extend the length of the oligonucleotide molecules of the first group, and where each of the oligonucleotage molecules of the second group varies from each other in just one base position and where each of the oligonucleotide molecules of the second group are each tagged with a fluorochrome which radiates light at a predetermined wavelength; and d) comparing the light patterns radiating from the predetermined positions on the substrate with predetermined light patterns emitted from identical substrates which were contacted with the disease associated alleles.