Patent ID: 8557289
Filing Date: 2013-10-15
Classification: A61K,A61N,A61P,B82Y,C12N

Abstract:
1. An ex vivo method of delivering a DNA or RNA molecule into a membrane enclosed cell, the method comprising: providing a sample comprising at least one membrane enclosed cell and at least particle suitable for the delivery of the DNA or RNA molecule out of a membrane enclosed cell organelle through heat induced release of said DNA or RNA molecule, the particle comprising a superparamagnetic core capable of generating heat in a magnetic alternating field encapsulated in a heat sensitive coating where to said DNA or RNA molecule substance and a membrane disruptive agent are associated, wherein said heat sensitive coating comprises a micelle like structure in direct contact with said core, wherein said micelle like structure is built up by at least one of hydrophobic molecule and at least one molecule containing both a hydrophobic and a hydrophilic moiety, wherein said hydrophilic moiety contains lipids, wherein the size of the superparamagnetic core is between 8 and 15 nm, wherein the particle is absorbed into the cell by endocytosis in the form of an endosome within the cell; applying a magnetic alternating field to said sample, whereby thermal energy of the particle core causes the heat sensitive coating of said particle to decompose, thereby releasing the DNA or RNA molecule and the membrane disruptive agent from the particle, wherein said magnetic field has an alternating field direction of a frequency in the range of 0.1 to 5 MHz; rupturing the endosome due to interaction of the membrane disruptive agent and the endosome membrane; wherein the DNA or RNA molecule is delivered into the cell cytoplasm and the genetic code and/or metabolism of the host cell is modified with the DNA or RNA molecule.