Patent ID: 7993880
Filing Date: 2011-08-09
Classification: C12Q

Abstract:
1. A method of targeted amplification of nucleic acids comprising: contacting a sample containing one or more target nucleic acid sequences with one or more precircle probes, wherein each precircle probe comprises at least a first and second targeting domain which hybridize to a target nucleic acid sequence and which flank at least one universal priming sequence, under conditions appropriate for hybridization of the first and second targeting domains of a precircle probe with a target nucleic acid sequence; extending the precircle probe with a polymerase such that the target nucleic acid sequence is copied, thereby producing an extended precircle probe; ligating one end of the extended precircle probe to the other end to create a closed circular probe; optionally removing linear nucleic acid sequences remaining in the sample; cleaving the closed circular probe prior to amplification; and amplifying the cleaved circular probe to produce amplified target nucleic acid sequence.