Patent ID: 9187777
Filing Date: 2015-11-17
Classification: C12N,C12P

Abstract:
1. A method for synthesizing a nucleic acid having a predefined sequence, the method comprising: a) providing a support comprising a plurality of features, each feature comprising a plurality of single-stranded support-bound oligonucleotides; b) hybridizing a partially double-stranded first oligonucleotide to at least a first support-bound oligonucleotide at a first feature, wherein the first partially double-stranded oligonucleotide comprises a 5′ overhang and a ligatable predetermined addition nucleotide at a 3′ end of the double-stranded portion; c) ligating the partially double-stranded first oligonucleotide to the first support-bound oligonucleotide thereby generating a first ligation product comprising the predetermined addition nucleotide; and d) cleaving the first ligation product thereby generating a first elongated support-bound oligonucleotide comprising the predetermined addition nucleotide.