Patent ID: 9194006
Filing Date: 2015-11-24
Classification: C12Q

Abstract:
1. A method for identifying the presence or absence of a target nucleic acid from a microorganism in a biological sample obtained from a human, said method comprising: (a) contacting the biological sample with a reaction mixture containing a DNA polymerase and a buffer to form a sample mixture under conditions suitable for amplification of the target nucleic acid from the biological sample without extracting the target nucleic acid from the biological sample; (b) thermocycling the sample mixture from step (a) such that the target nucleic acid, if present, is amplified; and (c) detecting the amplified target nucleic acid, if present, produced from step (b), wherein the biological sample is selected from the group consisting of whole blood, plasma, serum, and cerebrospinal fluid (CSF), wherein the nucleic acid in the biological sample is not extracted from the biological sample prior to amplification; wherein the biological sample is not diluted prior to contacting the biological sample with the reaction mixture; wherein 20-30% of the total volume of the sample mixture following step (a) is the biological sample; wherein the target nucleic acid is not present in a biological sample that does not contain the microorganism; and wherein the reaction mixture contains a cationic surfactant.