Patent ID: 7495148
Filing Date: 2009-02-24
Classification: A01H,C12N,Y02A

Abstract:
1. A method for molecular control of gene containment in sexually reproducing transgenic plants by providing a plant with a recoverable block of function (RBF) system, said system comprising: a first blocking construct (BC1) and a second blocking construct (BC2), said blocking constructs having a capacity to block at least one molecular or physiological function essential for development or reproduction of the transgenic plant, thereby leading to death or incapacity of sexual reproduction, said blocking constructs each comprising a blocking gene driven by an embryo-or germination specific promoter, said blocking gene being isolated and purified or artificial, and coding for Barnase protein; a transgene of interest (TGI) encoding desired gene products, said TGI being isolated and purified or artificial gene further being inserted between said two BCs; and a means to recover the functions blocked by the BCs, said means comprising at least one recovering construct (RC) comprising a recovering gene encoding for Barstar protein, said RC locating in a different chromosome apart from said BCs and said TGI and being capable to recover the blocked functions under homozygous condition introduced by intraline hybridization.