Patent ID: 7524635
Filing Date: 2009-04-28
Classification: A61K,A61P,C07K,G01N

Abstract:
1. A method for detecting the presence or amount of one or more BNP related species of interest or one or more pro-BNP related species of interest in a sample obtained from a subject, comprising: (a) assaying said sample to provide an assay result related to the presence or amount of said BNP related species of interest in said sample, wherein said assay is sensitive to degradation of the amino terminus of BNP; or (b) assaying said sample to provide an assay result related to the presence or amount of said pro-BNP related species of interest in said sample, wherein said assay is sensitive to degradation of the amino terminus of pro-BNP; or performing both steps (a) and (b) wherein said assay in step (a), if performed, is configured such that BNP is detected with at least a 5-fold reduction in signal relative to equimolar amounts of one or more BNP fragments in which the amino terminus has lost from 1 to 9 residues, and wherein said assay in step (b), if performed, is configured such that pro-BNP is detected with at least a 5-fold reduction in signal relative to equimolar amounts of one or more pro-BNP fragments in which the amino terminus has lost from 1 to 10 residues.