Patent ID: 7274809
Filing Date: 2007-09-25
Classification: G06K,G06T

Abstract:
1. A computer-implemented method of photometrically correcting digital images for the analysis of cellular features, comprising: a) providing at least one digital image of a sample of DNA stained cells, the sample comprising enough cells to determine a reference population in said sample of cells having a certain DNA content, the digital image comprising a plurality of individual pixels; b) determining a photometric correction factor that adjusts an optical measurement value of the certain DNA content cells of the sample substantially to a predetermined target value corresponding to optical measurements within a reference data set, wherein the photometric correction factor is determined based on a ratio of an experimental constant to an optical measurement value of the certain DNA content derived from the reference population determined from the sample; c) applying the photometric correction factor to individual pixels within said plurality of individual pixels, said individual pixels comprising desired pixels of the digital image for each desired cell in the sample to provide a normalized digital image; d) calculating at least one optical feature from the normalized digital image.