Patent ID: 6143495
Filing Date: 2000-11-07
Classification: C12Q

Abstract:
A method of amplifying nucleic acid sequences, the method comprising,(a) mixing a specific binding molecule with a target sample comprising a target molecule wherein a rolling circle replication primer is coupled to the specific binding molecule, wherein the specific binding molecule is bound to the target molecule,(b) mixing the rolling circle replication primer with an amplification target circle, to produce a primer-ATC mixture, and incubating the primer-ATC mixture under conditions that promote hybridization between the amplification target circle and the rolling circle replication primer in the primer-ATC mixture,wherein the amplification target circle comprises a single-stranded, circular DNA molecule comprising a primer complement portion, wherein the primer complement portion is complementary to the rolling circle replication primer, and(c) mixing DNA polymerase with the primer-ATC mixture, to produce a polymerase-ATC mixture, and incubating the polymerase-ATC mixture under conditions that promote replication of the amplification target circle,wherein replication of the amplification target circle results in the formation of tandem sequence DNA.