Patent ID: 6958213
Filing Date: 2005-10-25
Classification: C12N

Abstract:
1. A method for generating a polynucleotide sequence or population of sequences from parent single stranded polynucleotide sequences encoding one or more protein motifs, comprising the steps of: a) providing single stranded polynucleotide sequences constituting plus and minus strands of parent polynucleotide sequences; b) digesting the single stranded polynucleotide sequences with an exonuclease to generate a first population of single stranded fragments comprising fragments generated from the plus strands and a second population of single stranded fragments comprising fragments generated from the minus strands, said second population being separate from said first population; c) contacting said first population of single stranded fragments with said second population of single stranded fragments, whereby annealing occurs between said first population of single stranded fragments and said second population of single stranded fragments; d) amplifying the fragments that anneal to each other to generate at least one polynucleotide sequence encoding one or more protein motifs having altered characteristics as compared to the one or more protein motifs encoded by said parent polynucleotides.