Patent ID: 8236493
Filing Date: 2012-08-07
Classification: B01J,C12Q,C40B

Abstract:
1. A method for identifying a base, the method comprising: a) hybridizing: b) hybridizing the target-oligonucleotide probe hybrid complexes with a pool of labeled, ligatable oligonucleotide probes of a preselected length to form complexes comprising labeled, ligatable probes hybridized to the 3′ overhanging region of the hybridized target nucleic acid adjacent to the 5′ end of the oligonucleotides; c) ligating the labeled ligatable oligonucleotide probes to the 5′ end of the oligonucleotides with a ligase thereby forming labeled oligonucleotides on the substrate; d) washing the substrate to remove target nucleic acids and unligated labeled, ligatable probes; and e) detecting the presence of labeled oligonucleotides on the substrate to identify which base has specifically interacted with the target nucleic acid as an indication of a subsequence that is complementary.