Patent ID: 8420369
Filing Date: 2013-04-16
Classification: A23K,A23L,C12N,C12Y

Abstract:
1. A recombinant DNA molecule encoding a polypeptide having phytase activity after expression in a prokaryotic or eukaryotic host cell, wherein the recombinant DNA molecule has a DNA sequence encoding a polypeptide that has phytase activity and is obtained by variation of mature wild-type Escherichia coli phytase sequence SEQ ID NO: 10, wherein the variation is selected from the group consisting of: i) K74D of SEQ ID NO:10; ii) N139R and D142E of SEQ ID NO:10; iii) L1451 and L1981 of SEQ ID NO:10; and iv) V200P of SEQ ID NO:10; and v) variants due to the degeneracy of the genetic code that encode the amino acid sequence of SEQ ID NO:10; and wherein the polypeptide has an increased temperature and protease stability of phytase activity.