Patent ID: 6777191
Filing Date: 2004-08-17
Classification: A61K,C07K

Abstract:
A method of diagnosing the presence of viral infection, comprising:(a) contacting a biological sample suspected of containing a virus with a detectably labeled and soluble ICAM-1 functional derivative for a time and under conditions sufficient for the formation of detectable complexes of said ICAM-1 functional derivative and said virus; and (b) determining the presence of said complexes in said sample, wherein (i) said virus is a major serotype rhinovirus; (ii) said soluble functional derivative of ICAM-1 lacks a transmembrane domain and comprises (1) domains 1-2 of ICAM-1 or (2) a variant of domains 1-2 of ICAM-1; and (iii) said soluble functional derivative of ICAM-1 is capable of binding major serotype human rhinovirus; wherein said soluble functional derivative of ICAM-1 contains the following amino acids at the indicated position: Q1T, V4, R13, D26QPK, K39KE, G46NN, R49KV, Q58EDS, D60, D71GQS, K77T, E87, N103, A155N, R166PQ, N175TSA and S177; wherein said amino acids are defined in terms of native ICAM-1 consisting of the amino acid sequence of FIG. 1.