Patent ID: 7402391
Filing Date: 2008-07-22
Classification: C12Q

Abstract:
1. A method for detecting species in the genus Triticum in cases where even one species of the genus Triticum is contained in a subject to which the method is to be applied such as a food ingredient or product, which comprises the steps of: a) collecting 45S rRNA precursor gene sequences containing a ITS sequence of the genus b) selecting the base specific to the genus c) designing primers (A) and/or (B) having the specific base at 3′ end thereof, which primers can hybridize under the stringent conditions to a nucleic acid molecule having a common nucleotide base sequence for all species in the genus d) isolating DNA from the subject to which the method is to be applied; e) conducting PCR amplification for the isolated DNA using at least one member selected from the group consisting of the primers (A) and (B); and f) identifying the presence of the resulting amplification product from PCR containing at least a part of ITS-1 or ITS-2 sequence of the genus