Patent ID: 8187813
Filing Date: 2012-05-29
Classification: B01L,C12Q

Abstract:
1. A method for amplifying a target nucleic acid sequence by polymerase chain reaction (PCR), comprising the steps of: (1) providing a tube-like container; (2) placing a PCR sample in the tube-like container, wherein the sample contains a template DNA having the target nucleic acid sequence to be amplified, DNA polymerases, deoxyadenosine triphosphates (dATPs), deoxycytidine triphosphates (dCTPs), deoxyguanosine triphosphates (dGTPs), deoxythymidine triphosphates (dTTPs), and at least two oligonucleotide primers having a sequence complementary to the 3′ end of the target nucleic acid sequence wherein the primers are designed to have a melting temperature (T (3) embedding the bottom part of the container in a heat source, and then heating the PCR sample to allow the primers melt and maintaining a steady temperature in ° C. of the surface of the PCR sample (Ts) in the container less than the temperature in ° C. of the melting temperature of the primers (T