Patent ID: 6022733
Filing Date: 2000-02-08
Classification: C12M,G01N

Abstract:
A system for assessment of simulated biological dissolution of a pharmaceutical formulation and absorption of a pharmaceutically active compound therefrom, which system comprises:a dissolution chamber containing a pharmaceutical formulation within a medium to be provided to the apical surface of a cell monolayer;an analysis device in flow communication with the chamber for determining the dissolution profile of the pharmaceutical formulation;a cell culture chamber wherein the absorption of the pharmaceutically active compound by the cell monolayer may occur, said cell chamber comprises:i) a housing;ii) a rotatable tubular filter which is permeable to medium and capable of supporting the cell monolayer on its interior surface, said filter positioned within said housing forming an apical chamber within the interior of the filter, and a basal chamber between the filter and the wall of the housing, said filter capable of rotation about an axis within the housing substantially parallel to the direction of media flow through the basal and apical chambers;iii) means provided on said housing for the supply and outflow of basal medium to the basal chamber and apical medium to the apical chamber; andiv) means for analyzing the medium flowing out from the basal chamber;whereby the medium flowing from the dissolution chamber containing the pharmaceutical formulation is provided to the apical chamber and the rate of appearance of the pharmaceutically active compound in the medium flowing out from the basal chamber is analyzed to determine absorption of the compound.