Patent ID: 6548735
Filing Date: 2003-04-15
Classification: A01K,C07K,C12N,C12Y

Abstract:
A method for producing a heterologous protein, the method comprising expressing said heterologous protein in the milk of a non-human transgenic placental mammal and harvesting the heterologous protein, wherein said non-human transgenic placental mammal has integrated into its genome a genetic construct comprising(a) a 5â€²-flanking sequence from a mammalian milk protein gene and (b) DNA coding for said heterologous protein other than said milk protein, wherein said DNA comprises at least one whole, but not all, of the introns naturally occurring in the gene coding for said heterologous protein and wherein said 5â€²-flanking sequence controls expression of said heterologous protein, said genetic construct being integrated into said non-human transgenic placental mammal's genome in such a way that said DNA is expressed in a mammary gland of said mammal at levels greater than those achieved by expression of a corresponding intron-less construct.