Patent ID: 8962329
Filing Date: 2015-02-24
Classification: A61K,A61P,C07D,C07K,C12N,C12P

Abstract:
1. An isolated nucleic acid molecule comprising the sanglifehrin A biosynthesis gene cluster of SEQ ID NO: 1, wherein: (i) sfaK (residues 97396-101943 of SEQ ID NO: 1), encoding a polypeptide of SEQ ID NO: 14, has been deleted or inactivated, whereby the linear polyketide synthase gene sfaK is knocked out; and/or (ii) sfaA (residues 17024-17854 of SEQ ID NO: 1), encoding a polypeptide of SEQ ID NO: 4, has been deleted or inactivated, whereby the phenylalanine hydroxylase gene sfaA is knocked out.