Patent ID: 8476492
Filing Date: 2013-07-02
Classification: C12N,C12Y

Abstract:
1. A method of utilizing the pts gene and antisense ads gene to increase patchouli alcohol content in Artemisia annua L., said method comprising a) obtaining the complete open reading frame sequence of artemisinin biosynthesis pathway key enzyme ads gene of SEQ ID NO: 1, and the complete open reading frame sequence of the patchouli alcohol biosynthesis pathway key enzyme pts gene, set forth in SEQ ID NO: 2, linked to the plastid targeted signal peptide of the b) constructing plant expression vectors that contain the ads open reading frame in antisense orientation linked to transcriptional regulatory sequences, and the plastid targeted pts gene linked to transcriptional regulatory sequences; c) transforming d) transforming e) determining the patchouli alcohol content in the transgenic