Patent ID: 6306577
Filing Date: 2001-10-23
Classification: C12Q,G01N,Y02A,Y10S

Abstract:
A method for measuring an enzyme reaction to determine an amount of a substance involved in the enzyme reaction wherein said substance is endotoxin, (1.fwdarw.3)-.beta.-D-glucan, or peptidoglycan, and said enzyme reaction is a Limulus reaction or a phenol oxidase precursor cascade reaction, which method comprises the steps of:preselecting a parameter of the enzyme reaction, said parameter being indicative of a time course of the enzyme reaction,starting the enzyme reaction,measuring a time required for the parameter of the enzyme reaction to change from a first threshold value to a second threshold value, said first threshold value being set within 0.1 to 10% of a maximum change in the parameter of the enzyme reaction, andcorrelating the measured time to an amount of the substance involved in the enzyme reaction.