Patent ID: 6376749
Filing Date: 2002-04-23
Classification: A23L,C12N

Abstract:
A method of controlling the starch fine structure of starch derived from a grain of corn comprising:(a) preparing a chimeric gene comprising a nucleic acid fragment sufficient to suppress the endogenous expression of a corn starch branching enzyme I or II, wherein said nucleic acid fragment comprises at least a portion of corn starch branching enzyme I or II, operably linked in sense or antisense orientation on the upstream side to a promoter that directs gene expression in corn endosperm tissue, and operably linked on the downstream side to a regulatory sequence for transcriptional termination, and (b) transforming corn with the chimeric gene of step (a), wherein expression of said chimeric gene results in alteration of the fine structure of starch derived from the grain of said transformed corn compared to the fine structure of starch derived from corn not possessing said chimeric gene.