Patent ID: 7749749
Filing Date: 2010-07-06
Classification: C12M

Abstract:
1. A bioreactor for generating uniform distribution of shear stress, comprising: a cone, having a cone surface with an outline of modified catenary; a container, having a fixed plate at the bottom inside the accommodating space of said container, and said fixed plate comprises a plurality of reservoirs; wherein the cone tip located above the center of said fixed plate is capable of loading culture media into the accommodating space of said container; and more uniform shear stress can generate in the culture media to act on the sample in said reservoirs of the fixed plate when the cone rotates and makes the culture media run; wherein the outline of said cone surface is derived from the following formulas (1) and (2): wherein Z(r) is the distance between a point on said fixed plate, which located on an imaginary circle whose center point is the center of said fixed plate and its radius is r, and another point on said cone surface right above said point; h is the distance between the cone tip and the fixed plate when the cone is placed in the center of the fixed plate; α is the cone tip angle; C is a constant and C≠1; value a is derived from the formula (2) as follow by using the cone radius R, said distance h, and said cone tip angle α: