Patent ID: 8404449
Filing Date: 2013-03-26
Classification: C12Q,G01N

Abstract:
1. A method for sensing an individual molecule of at least one reactive analyte in a solution comprising: providing a sensing device separated into a trans chamber and a cis chamber by a divider, wherein an engineered protein pore is operably disposed in the divider; providing a detection system operable to detect a current between the cis and trans chambers; providing an ionic solution containing at least one reactive analyte capable of covalently bonding to the protein pore within the lumen of the pore and thereby altering the current between the cis and trans chambers to the cis or trans chamber; and detecting the current between the cis and trans chambers, wherein a change in the current between the cis and trans chambers indicates whether the analyte is covalently bound to the protein pore; and using the detected current, stochastically sensing the at least one analyte.