Patent ID: 9068167
Filing Date: 2015-06-30
Classification: A61P,C12N

Abstract:
1. A method for culturing pluripotent stem cells to obtain foci of cells undergoing an epithelial-to-mesenchymal transition, the method comprising the steps of: providing pluripotent stem cells as a monolayer on a support; applying a matrix layer to the apical surface of pluripotent stem cells of the monolayer, wherein both the support and the matrix layer contact the pluripotent stem cells; and culturing the pluripotent stem cells contacted by the matrix layer to induce the contacted pluripotent stem cells to form multilayered foci of cells undergoing the epithelial-to-mesenchymal transition, wherein culturing comprises providing Activin A to the pluripotent stem cells contacted by the matrix layer, and wherein cells undergoing the epithelial-to-mesenchymal transition comprise N-cadherin-positive mesenchymal cells.