Patent ID: 8093067
Filing Date: 2012-01-10
Classification: G01N

Abstract:
1. Method for the demonstration of a specific complex formed by reacting an antibody present in a solution and an antigen bound to a magnetic particle, the reaction taking place in a reactor with an open top and sealed base whose diameter decreases in the area close base to the base in such a way that it forms an inclined wall extending down to the base, said inclined wall being at least partially coated with an anti-immunoglobulin or any other compound capable of binding to the antibody of said formed complex, wherein it consists of the following steps: a) prior to the reaction, preliminary filling of the reactor with a viscous substance or homogeneous gel such that at least part of the inclined wall of the reactor is coated, b) contacting the solution containing or likely to contain said antibody with the magnetic particle suspension carrying or likely to carry said antigen at a point above the viscous solution in the reactor, c) incubation of the reactor, preferably for the period of time and temperature required for the formation of the complex, d) application of a magnetic field to said reactor and stirring of the reactor such that the magnetic particles are either drawn towards the base and/or inclined wall of the reactor, and e) reading with the naked eye and/or by any other suitable reading system of the image obtained at the base of the reactor and/or inclined wall of the reactor coated with said anti-immunoglobulin or any other compound capable of binding to the antibody, the image obtained making it possible to demonstrate the presence or otherwise of a specific antibody/antigen complex.