Patent ID: 6884597
Filing Date: 2005-04-26
Classification: C07K,C12Q,G01N,Y10S

Abstract:
1. A method for screening a compound that inhibits or enhances activity of an acetyltransferase to catalyze a reaction that transfers an acetyl group from one substrate to another, the method comprising: (a) contacting the acetyltransferase with a peptide substrate in a presence of a test compound, (b) detecting an amount of an acetylated peptide substrate using an anti-acetylated peptide antibody, wherein an effective amount of the anti-acetylated peptide antibody recognizes with specificity only an acetylated form of the peptide substrate and does not recognize the peptide substrate in its unacetylated form, (c) comparing the amount of the acetylated peptide substrate detected in step (b) with a control amount defined as an amount of an acetylated peptide substrate detected in an absence of the test compound, and (d) selecting the compound associated with an increase or decrease in the amount of the acetylated peptide substrate as compared to the control amount.