Patent ID: 6649812
Filing Date: 2003-11-18
Classification: C12N

Abstract:
A method for producing a transgenic sunflower, said method comprising:a) genetically transforming a meristematic explant from said sunflower; b) culturing said transformed meristematic explant on a selection medium; c) detecting among axillary buds and/or newly formed leaf buds, the buds which are transformed; d) removing said transformed buds detected in step c) from the cultured transformed meristematic explant; e) culturing said transformed buds removed in step d) on a selection medium; f) repeating steps c), d) and e) at least three times; and, g) regenerating said cultured transformed buds to obtain a transgenic sunflower, wherein at least 92% of transgenic sunflowers are wholly transformed in the T0 generation.