Patent ID: 6093802
Filing Date: 2000-07-25
Classification: A61K,A61P,C07K,Y10S

Abstract:
A method for obtaining substantially purified glial cell line-derived neurotrophic factor protein, comprising:culturing cells which are transformed to express a neurotrophic factor protein selected from the group consisting of:(a)Ser Pro Asp Lys Gln Met Ala Val Leu Pro Arg Arg Glu - Arg Asn Arg Gln Ala Ala Ala Ala Asn Pro Glu Asn Ser - Arg Gly Lys Gly Arg Arg Gly Gln Arg Gly Lys Asn Arg - Gly Cys Val Leu Thr Ala Ile His Leu Asn Val Thr Asp - Leu Gly Leu Gly Tyr Glu Thr Lys Glu Glu Leu Ile Phe - Arg Tyr Cys Ser Gly Ser Cys Asp Ala Ala Glu Thr Thr - Tyr Asp Lys Ile Leu Lys Asn Leu Ser Arg Asn Arg Arg - Leu Val Ser Asp Lys Val Gly Gln Ala Cys Cys Arg Pro - Ile Ala Phe Asp Asp Asp Leu Ser Phe Leu Asp Asp Asn - Leu Val Tyr His Ile Leu Arg Lys His Ser Ala Lys Arg - Cys Gly Cys Ile of SEQ ID NO:6; and (b) a neurotrophic factor protein having at least the following properties of SEQ ID NO: 6(i) a specific activity of at least about 12,000 TU/ug;(ii) a molecular weight on reducing SDS-PAGE of about 20-23 kD;(iii) a molecular weight on non-reducing SDS-PAGE of about 31-42 kD; and(iv) a specific activity of at least about 24,000 times greater than the specific activity of B49-conditioned medium; and isolating the neurotrophic factor protein.