Patent ID: 6783984
Filing Date: 2004-08-31
Classification: A01N

Abstract:
A process for the cryo-preservation of a primary regeneration tissue derived from cocoa plant or a coffee plant comprising the following steps:cultivating a plant tissue derived from cocoa plant or a coffee plant on an induction medium for a time sufficient to induce a primary regeneration tissue comprising embryogenic cells; culturing the primary regenerating tissue on a multiplication medium for a time sufficient to maintain a stable proliferation of the primary regeneration tissue; treating the primary regeneration tissue in a two step process that comprises sequential incubation in first and second sucrose media, wherein the second medium contains a greater amount of sucrose than the first medium; prefreezing the primary regenerating tissue to a temperature between âˆ’20Â° C. and âˆ’40Â° C.; and cryofreezing the primary regeneration tissue.