Patent ID: 6410259
Filing Date: 2002-06-25
Classification: C12N,C12P,Y10S

Abstract:
A process for the production of an N-deacylated cephalosporin compound comprising the steps of:(a) fermenting a microbial strain capable of &bgr;-lactam production and expressing acyltransferase as well as expandase activity, and optionally acetyltransferase and/or hydroxylase activity, in the presence of a side chain precursor according to formula (1) HOOCâ€”Xâ€”COOHâ€ƒâ€ƒ(1) â€ƒwherein X is (CH2)mâ€”CH&boxH;Aâ€”(CH2)n or (CH2)mâ€”Câ‰¡Câ€”(CH2)n, wherein m and n each individually are 0, 1, 2 or 3 and m+n=2 or 3, and A is CH or N, or X is (CH2)pâ€”CH&boxH;CHâ€”CH&boxH;Câ€”(CH2)q, wherein p and q each individually are 0 or 1 and p+q =0 or 1, â€ƒor a salt, ester or amide thereof, said side chain precursor yielding an acyl-6-APA derivative incorporating said precursor, said acyl-6-APA derivative being in situ expanded to the corresponding acyl-7-ADCA derivative, optionally further reacted to the acyl-7-ADAC or acyl-7-ACA derivative, and (b) recovering the acyl-7-cephalosporin derivative from the fermentation broth (c) deacylating said acyl-7-cephalosporin derivative, and (d) recovering the crystalline N-deacylated cephalosporin compound.