Patent ID: 6171798
Filing Date: 2001-01-09
Classification: C12Q,G01N

Abstract:
A method of diagnosing cancer or determining p53 status in a sample suspected of being neoplastic, comprising the steps of:comparing the level of expression of an RNA transcript or its translation product in a first sample of a first tissue to the level of expression of the transcript or translation product in a second sample of a second tissue, wherein the first tissue is suspected of being neoplastic and the second tissue is a normal human tissue, wherein the first and second tissue are of the same tissue type, and wherein the transcript is a transcript of a gene selected from the group consisting of IG GAMMA-1 CHAIN C REGION (HUMAN), Human 1 g gamma3 heavy chain disease OMM protein mRNA, 274912 MYELIN TRANSCRIPTION FACTOR 1 (Homo sapiens), 152524 CYCLIN-DEPENDENT KINASE INHIBITOR 1 (Homo sapiens), H. sapiens mRNA for 43 kDa inositol polyphosphate 5-phosphatase, Human N-acetylgalactosamine 6-sulphatase (GALNS) gene, exon 14, 155730 KERATIN, TYPE I CYTOSKELETAL 17 (HUMAN), H. sapiens mRNA (clone 9112), kinase related protein, Human placental cDNA coding for 5'nucleotidase (EC3.1.3.5), H. sapiens MLN62 mRNA, Human activated p21cdc42Hs kinase (ack) mRNA, complete cds., 81780 COMPLEMENT C4 PRECURSOR (Homo sapiens), 172486 clone, mRNA for tuberin, or TSC2 gene, Homo sapiens of cardiac alpha-myosin heavy chain gene. KERATIN, TYPE II CYTOSKELETAL 5 (HUMAN); contains MSR1 repetive element, 182000 FK506-BINDING PROTEIN PRECURSOR (Mus musculus), Human insulin-like growth factor-binding protein-3 gene, complete Cds, clone HL1006d, 72466 ALPHA CRYSTALLIN B CHAIN (HUMAN), Homo sapiens mRNA for serum response factor-related protein, RSRFR2, 198656 HEPATOCYTE GROWTH FACTOR-LIKE PROTEIN PRECURSOR (Homo sapiens), 62461 SMALL NCUELAR RIBONUCLEORPORTEIN Particle N (SNRPN), contains MSR1 repetive element, 155335 INTEGRIN ALPHA-3 (Homo sapiens), 41792 TUBULIN BETA-2-CHAIN (HUMAN), Human estradiol 17 beta-dehydrogenase gene, complete cds, H. sapiens mRNA for cystathionine-beta-synthase, Homo sapiens of cardiac alpha-myosin heavy chain gene, Human mRNA for thyroglobulin, Human guanine nucleotide regulatory protein (ABR) mRNA, complete cds, Human novel growth factor receptor mRNA 3' Cds, 142899 DNA-DIRECTED RNA POLYMERASE III LARGEST SUBUNIT (Plasmodium falciparum), Human mRNA for collagen VI alpha-1 C-terminal globular domain, Homo sapiens sodium channel type I, beta subunit (SCN1B) mRNA, complete cds., 50887 GUANINE NUCLEOTIDE DISSOCIATION STIMULATOR RALGDSA (Mus musculus), Human cytochrome P450 4F2 (CYP4F2) mRNA, complete cds, Human MAGE-2 gene exons 1-4, complete cds, H. sapiens mRNA for tansforming growth factor alpha, 38251 H sapiens HSJ1 mRNA, p53, H. sapiens mRNA for intestine-specific annexin, KERATIN, TYPE ICYTOSKELETAL 15 (HUMAN); contains MER20 repetitive element 5065 GROWTH ARREST AND DNA-DAMAGE-INDUCIBLE PROTEIN GADD45 (Homo sapiens), Human steroidogenic acute regulatory protein (StAR) mRNA, complete cds, LYSYL HYDROXYLASE (PLOD), (HUMAN); 151767 FASL RECEPTOR PRECURSOR (Homo sapiens), Homo sapiens creatine transporter mRNA, complete cds, KERATIN TYPE I CYTOSKELETAL 15 (HUMAN); contains MER20 repetitive element, Human mRNA for thyroperoxidase, 275040 HEPATOCYTE GROWTH FACTOR-LIKE PROTEIN PRECURSOR (HUMAN), 121731 CYTOCHROME P450 IVB1 (Rattus norvegicus), Homo sapiens dopamine transporter (SLC6A3) mRNA, complete cds, 139080 COMPLEMENT DECAY-ACCELERATING FACTOR 1 PRECURSOR (Homo sapiens), H. sapiens mRNA for caveolin, Human endogenoous retrovirus type C onccovirus sequence, 31481 TYROSINE-PROTEIN KINASE HCK (Homo sapiens), Homo sapiens ribosomal protein S6 kinase 2 (RPS6KA2) mRNA, complete cds, Human Fas antigen (fas) mRNA, complete cds, Human mRNA encoding pregastrin (a regulatory hormone of gastric acid secretin and growth of the gastrointestinal mucosa), H. sapiens mRNA for placental growth factor (PIGF), H. sapiens mRNA for placenta growth factor (PIGF), Human PML-2 mRNA, complet Cds, Human mRNA (KIAA0027) for ORF, partial cds, Human placenta-specific growth hormone mRNA, complete cds, Human high conductance inward rectifier potassium channel alpha subunit mRNA, complete cds, Human cytochrome P450 IID6 (CYP2D6) gene, complete cds, 180447 FIBROBLAST GROWTH FACTOR RECEPTOR 3 PRECURSOR (Homo sapiens), 153585 EBNA-2-NUCLEAR PROTEIN (Epstein-barr virus), 36678 TROPONIN C, ISOFORM 2 (Balanus nubilis), 182125 HDL-BINDING PROTEIN, HUMMLC2At; Homo sapiens; 593 base pairs, 80486 LIVER CARBOXYLESTERASE PRECURSOR (HUMAN), 121916 NEUTRAL CALPONIN, SMOOTH MUSCLE (Sus scrofa), Human 11 beta-hydroxysteroid dehydrogenase type II mRNA, complete cds, Homo sapiens interferon regulatory factor 1 gene, complete cds, Human Fas antigen (fas) mRNA, complete cds, NEUTROPHIL OXIDASE FACTOR (p67 PHOX) (HUMAN), Human 11 kd protein mRNA, complete cds, Human semaphorin V mRNA, complete cds, MYELIN TRANSCRIPTION FACTOR 1 (HUMAN), 175991 NEURONAL CALCIUM SENSOR 1 (Rattus norvegicus), 81422 HUMAN SMOOTH MUSCLE ALPHA-ACTIN (AORTIC TYPE), Human mRNA for irp protein (int-1 related protein), Human Fas antigen (fas) mRNA, complete cds, Human beta-migrating plasminogen activator inhibitor 1 mRNA 3' end, 26063 COMPLEMENT C4 PRECURSOR (Homo sapiens), 142450 VASCULAR ENDOTHELIAL GROWTH FACTOR PRECURSOR (Rattus norvegicus), Human visinin-like peptide 1 homolog mRNA, complete cds, H. sapiens mRNA for red cell anion exchanger (EPB3, Ae1, Band 3) 3' non-coding region, Human laminin S B3 chain (LAMB3) mRNA, complete cds, Human cytochrome P450 IID6 (CYP2D6) gene, complete cds, Human hexokinase 1 (HK1) mRNA, complete cds, Human mRNA for the MDM2 gene, Human mRNA for cytochrome P-450LTBV, RYANODINE RECEPTOR, SKELETAL MUSCLE (HUMAN), 39052 POTASSIUM CHANNEL PROTEIN EAG (Drosophila melanogaster), and 124416 SERINE THREONINE-PROTEIN KINASE COT-1 (Neurospora crassa);categorizing the first sample as neoplastic or as having a mutant p53 when expression is found to be lower in the first sample than in the second sample.