Patent ID: 8637230
Filing Date: 2014-01-28
Classification: A01N

Abstract:
1. A machine perfusion solution for maintaining donor organ viability consisting of a mammalian tissue culture medium selected from the group consisting of Minimal Essential Medium Eagle (MEM), Dulbecco's Modified Eagle Media (DMEM), RPMI 1640 Media, DMEM/F-12 Media, Hams F-10, Hams F12, Iscove's Modified Dulbecco's Medium, Leibovitz's L-15 Media, Minimum Essential media with Earle's Salts, and Williams medium E, wherein the tissue culture medium is modified to: (a) 10 to 50 g/L polyethylene glycol (PEG) having a molecular weight of 25 to 50 kDa (b) 1,000 to 10,000 mg/L HEPES; (c) 1,000 to 5,000 mg/L of each of raffinose and trehalose; (d) 1,000 to 5,000 mg/L of gluconic acid; (e) 0.7 to 1.8 g/L glutathione; (f) 0.00001 to 0.001 g/L vitamin E; (g) 0.01 to 0.1 g/L of ascorbic acid; and (h) 0.00001 to 0.001 g/L selenium; wherein the solution has: