Patent ID: 6248532
Filing Date: 2001-06-19
Classification: A61K,A61P,C07K

Abstract:
A method to identify modulators of mCREBa binding to DNA comprising the steps of:a) incubating mCREBa with a DNA sequence known to specifically bind mCREBa;b) determining the degree of binding between mCREBa and the DNA sequence;c) repeating step (a) in the presence of a putative modulator of mCREBa binding to the DNA sequences; andd) comparing binding of mCREBa with the DNA sequence in the presence of the putative modulator in step (c) to the degree of binding determined in step (b), and identifying the putative modulator as an enhancer when increased binding is detected in the presence of the putative modulator and identifying the putative modulator as a repressor when decreased binding in the presence of the putative modulator is detected.