Patent ID: 8258109
Filing Date: 2012-09-04
Classification: C12N

Abstract:
1. A method of modulating splicing of a LMNA pre-mRNA in a cell, comprising contacting the cell with an oligomeric compound comprising an oligonucleotide consisting of 12 to 30 linked nucleosides, wherein the oligonucleotide has a nucleobase sequence comprising an at least 8 nucleobase portion complementary to an equal length portion of nucleotides 1971-2050, 2015-2094 or 2013-2092 of SEQ ID NO: 4 and is at least 90% complementary to LMNA pre-mRNA having SEQ ID NO:4 as measured over the entirety of the oligonucleotide, wherein each nucleoside of the oligonucleotide comprises a modified sugar moiety; and thereby modulating splicing of the LMNA pre-mRNA in the cell.