Patent ID: 7247434
Filing Date: 2007-07-24
Classification: C07B,C07D,C07H,C12Q,C40B,Y10T

Abstract:
1. A method comprising: (a) providing nucleic acid fragments, each fragment having cleavably attached thereto a mass tag; (b) separating the tagged fragments on the basis of fragment charge, sequential length, size or shape; (c) placing a charge on the tag and cleaving the tag from the fragment, where the charge is placed on the tag prior to, during, or after cleaving the tag from the fragment; and (d) characterizing each tag by mass spectrometry, wherein at least one tagged nucleic acid fragment is a compound of the formula: wherein, T L is an organic group which allows a T X is a nucleic acid fragment attached to L at other than the 3′ end of the nucleic acid fragment; with the provisos that the compound is not bonded to a solid support nor has a mass of less than 250 daltons.