Patent ID: 6124435
Filing Date: 2000-09-26
Classification: C07D,C07K,G01N,Y10S

Abstract:
A conjugate molecule having a carboydrate-containing compound covalently attached to a thiol-containing compound via a heterobifunctional compound of formula I ##STR3## wherein the conjugate has a hydrazide moiety and a maleimide moiety, wherein the hydrazide and the maleimide moieties are separated by a spacer X,wherein X is an aliphatic hydrocarbon spacer of 6 to about 25 carbon atoms or consists of an aliphatic hydrocarbon chain and an alicyclic or aromatic moiety having a total number of 6 to about 25 carbon atoms,wherein X is covalently attached to the hydrazide or hydrazide derivative and X is covalently attached to the heteronitrogen of the maleimide,wherein the carbohydrate-containing compound is covalently attached to the hydrazide; andwherein the thiol-containing compound is covalently attached to a heterocyclic carbon atom of the maleimide via a thioether linkage.