Patent ID: 7192729
Filing Date: 2007-03-20
Classification: C12N,C12Q,C12Y,G01N

Abstract:
1. A method for assaying homocysteine (Hcy), S-adenosylhomocysteine (SAH) or adenosine in a sample, which method comprises: a) contacting a sample containing or suspected of containing Hcy, SAH or adenosine with a mutant SAH hydrolase derived from a SAH hydrolase, wherein said SAH hydrolase is encoded by a nucleic acid comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:185, SEQ ID NO:186, SEQ ID NO:187, and SEQ ID NO:188; wherein said mutant SAH hydrolase has binding affinity for Hcy, SAH or adenosine but has attenuated catalytic activity, and wherein said binding affinity and/or said attenuated catalytic activity of said mutant SAH hydrolase is caused by a mutation in said mutant SAH hydrolase's catalytic site, its binding site for NAD b) detecting binding between Hcy, SAH or adenosine with said mutant SAH hydrolase, whereby the presence or amount of Hcy, SAH or adenosine in said sample is assessed.