Patent ID: 7488581
Filing Date: 2009-02-10
Classification: C12Q

Abstract:
1. A method of detecting a target nucleic acid sequence comprising: providing a stem-and-loop structured nucleic acid for measurement wherein the nucleic acid comprises complementary sequence portions located at both terminals and a target sequence portion therebetween as well as a double-stranded portion formed by hybridization of the complementary sequence portions located at both terminals and a remaining looped single-stranded portion; providing a probe nucleic acid having a sequence complementary to the target sequence portion wherein one end of the probe nucleic acid being immobilized to a solid substrate surface; reacting the nucleic acid for measurement with the probe nucleic acid to specifically hybridize the target sequence portion of the nucleic acid for measurement to the probe nucleic acid; and detecting presence or absence of the nucleic acid for measurement hybridized to the probe nucleic acid by using a double strand specific intercalator, thereby detecting the target nucleic acid sequence.