Patent ID: 6329154
Filing Date: 2001-12-11
Classification: G01F,G01N,Y10S,Y10T

Abstract:
A histamine measuring method for determining a concentration of histamine comprising the steps of:preparing an oocyte which expresses histamine receptors and identifies specifically the presence of histamine;holding said oocyte in a recess at a bottom of a vessel in which a buffer solution is filled;infusing a sample into a fine reacting tube having an antigen immobilized on an inner surface thereof wherein said antigen stimulates histamine released from the sample infused into said fine reacting tube;transferring a solution containing histamine released from the sample in said fine reacting tube to said vessel to make contact with said oocyte in said vessel;detecting a change in an electric response of said oocyte before and after binding of histamine to the histamine receptors; anddetermining a concentration of histamine released from the sample in said fine reacting tube;wherein the concentration (A) of histamine released from the sample in said fine reacting tube is determined by means of a predefined calibration curve obtained by stimulating said oocyte with a plurality of known concentrations of histamine to detect electric responses of said oocyte to obtain a correlation between said electric responses and said plurality of known concentrations of histamine.