Patent ID: 9115354
Filing Date: 2015-08-25
Classification: C07K,C12N

Abstract:
1. A nucleic acid molecule encoding an R-type hmw bacteriocin, wherein the nucleic acid molecule is from a genome of a first strain of Clostridium difficile and comprises a polynucleotide sequence that encodes polypeptides that are at least 80% identical to SEQ ID NOs: 66-77, wherein the nucleic acid molecule further comprises a heterologous sequence encoding a receptor binding domain (RDB) of a prophage, or an RBD of a prophage remnant from the genome of a second strain of C. difficile , or an RBD of a bacteriophage, wherein the R-type hmw bacteriocin comprises a base plate attachment region (BPAR) comprised of a portion of the amino terminus of a first BPAR polypeptide that is at least 80% identical to the amino terminus of SEQ ID NO:78, and comprising a second polynucleotide encoding a portion of the carboxy terminus of a BPAR that is cognate to the RBD, and wherein the R-type hmw bacteriocin has bactericidal activity against a strain of C. difficile.