Patent ID: 9096843
Filing Date: 2015-08-04
Classification: A61P,C07K,C12N,C12P,C12Y

Abstract:
1. A method for producing an α-amidated peptide comprising: (i) allowing a target peptide with a C-terminal glycine residue to react with both a peptidylglycine alpha-hydroxylating monooxygenase (PHM) and a polypeptide consisting of amino acids 2-306 of SEQ ID NO: 1, amino acids 3-336 of SEQ ID NO: 2, amino acids 3-305 of SEQ ID NO: 3, or amino acids 3-279 of SEQ ID NO: 4, wherein said polypeptide has peptidyl-α-hydroxyglycine alpha-amidating lyase (PAL) activity under conditions suitable for enzymatic activity, and optionally further comprises 1-30 substitution(s) or deletion(s), and wherein the reaction with said PHM and said polypeptide on said target peptide is performed either in two separate steps or simultaneously; and (ii) recovering the C-terminally α-amidated peptide.