Patent ID: 6656694
Filing Date: 2003-12-02
Classification: C07D,G01N,Y10S

Abstract:
A method of identifying a ligand for a second binding site on a biological substrate, which comprisesdetermining the effect of a candidate compound on the binding of a probe compound which is known to bind to a first binding site on the biological substrate, in the presence of a first multibinding compound which is known to bind to the first and second binding site on the biological substrate, whereby the candidate compound may compete with the first multibinding compound for binding to the second binding site, so reducing the binding of the first multibinding compound to the biological substrate and so increasing the binding of the probe compound to the first binding site; and identifying as a ligand for the second binding site of the biological substrate a candidate compound that has the effect of increasing the binding of the probe compound to the first binding site of the biological substrate.