Patent ID: 6808874
Filing Date: 2004-10-26
Classification: G01N

Abstract:
A method for monitoring the activity of an enzyme, the method comprising the steps of:(a) providing a binding domain which includes a site for enzymatic modification; (b) providing a binding partner which binds to the binding domain in a manner which is dependent upon modification of the site; (c) contacting the binding domain with the enzyme; and (d) detecting binding of the binding domain to the binding partner as an indication of the activity of the enzyme; wherein one of said binding domain and said binding partner comprises a polypeptide and the other of said binding domain and said binding partner comprises a nucleic acid; and wherein said binding domain comprises a polypeptide and said site comprises a sequence which directs modification by at least one of the following enzymes: a carbohydrate transferase, a ubiquitin activating enzyme E1, a ubiquitin conjugating enzyme E2, a ubiquitin conjugating enzyme Ubc9, a ubiquitin protein ligase E3, a poly (ADP-ribose) polymerase, a fatty acyl transferase, and an NAD: Arginine ADP ribosyltransferase.