Patent ID: 6207384
Filing Date: 2001-03-27
Classification: C12N,C12Q

Abstract:
A method for locating an essential region in a portion of the genomic DNA from the genome an organism, said method comprising:a) mutagenizing DNA having the sequence of said portion of genomic DNA, said mutagenizing using in vitro mutagenesis with a transposon, wherein said mutagenizing is sufficient to mutagenize a region of an essential gene, resulting in a transposon insertion frequency in said portion of genomic DNA of at least 3 insertions per kilobase of target DNA;b) transforming cells of said organism with the mutagenized DNA of step a), wherein said cells have a haploid growth phase;c) identifying cells containing said mutagenized DNA; andd) locating said essential region of said portion of genomic DNA by detecting the absence of transposons in said essential region in the mutagenized cells containing said mutagenized DNA.