Patent ID: 8846336
Filing Date: 2014-09-30
Classification: C12Q,G01N

Abstract:
1. A target microbe-specific test mixture for detecting the presence or absence of a target microbe in a test sample in form as obtained from a biological, environmental, or food specimen, the test mixture comprising: a metabolizable substrate in an amount that is sufficient to support log phase growth of the target microbe until a detectable characteristic signal is produced in the test mixture and sample admixture, which metabolizable substrate is adapted to produce the detectable characteristic signal when metabolized by the target microbe; an amount of vitamin, amino acid, element and salt ingredients, which amount of ingredients is operable to allow viability of the target microbe in the presence of the metabolizable substrate and to aid the target microbe through lag phase and into log phase reproduction of the target microbe in the test sample; and organic micro particles in a form that promotes the formation of a microbial biofilm; wherein the micro particles are in an amount that is effective to accelerate the formation of microbial biofilms within an admixture of the test sample in form as obtained from a biological, environmental, or food specimen and the test mixture; and wherein the test mixture is incompetent of supporting continued growth of any viable non-target microbes in the admixture in an amount that would interfere with the detection of the target microbe.