Patent ID: 9157109
Filing Date: 2015-10-13
Classification: B05D,C12Q,G01N

Abstract:
1. A biosensor for the detection of an intrinsic or recombinant β-glucuronidase or β-galactosidase enzyme comprising: (a) a substrate having a first and second end; (b) a reaction zone immobilized on the substrate comprising layers that are printed on the substrate using inkjet printing and are arranged in order beginning adjacent to the substrate of: (i) a first biomolecule compatible sol gel layer; (ii) a chromogenic substrate for the enzyme; and (iii) a second biomolecule compatible sol gel layer; wherein immersion of the first end of the substrate in a solution comprising or suspected of comprising the enzymes, results in lateral flow of the solution from the first end of the substrate to the second end of the substrate by capillary action, the flow passes through the reaction zone where the enzymes, if present, react with the chromogenic substrate, the chromogenic substrate is one that, when reacted with the enzyme produces a colored product that is detected, and detection of the coloured product indicates that the solution comprised the enzymes; or wherein overspotting a solution comprising or suspected of comprising the enzymes on to the reaction zone results in the enzymes, if present, reacting with the chromogenic substrate, the chromogenic substrate is one that, when reacted with the enzyme produces a colored product that is detected, and detection of the coloured product indicates that the solution comprised the enzymes; or wherein dipping the biosensor into a solution comprising or suspected of comprising the enzymes results in the enzymes, if present, reacting with the chromogenic substrate, the chromogenic substrate is one that, when reacted with the enzyme produces a colored product that is detected, and detection of the coloured product indicates that the solution comprised the enzymes.