Patent ID: 9089600
Filing Date: 2015-07-28
Classification: A61B,A61F,A61K,A61L,G01N,G06T

Abstract:
1. A computer-implemented method of analyzing in vivo an implanted substrate comprising stem cell-derived retinal pigment epithelium (RPE) cells, the method comprising: directing by a light source narrow-band light at the substrate; detecting by a detector fluorescence that is emitted from fluorophores coupled to the stem cell-derived RPE cells on the substrate; generating by a computer system a plurality of two-dimensional images of the substrate on an x-y plane at a plurality of focal depths along the z axis; combining by the computer system the plurality of two-dimensional images on the x-y plane to generate a single image; determining by the computer system a first number of stem cell-derived RPE cells in the single image; calculating by the computer system a first density of stem cell-derived RPE cells in the single image based on the determined first number of stem cell-derived RPE cells; comparing by the computer system the first number of stem cell-derived RPE cells and the first density of stem cell-derived RPE cells to a second number of stem cell-derived RPE cells and a second density of stem cell-derived RPE cells; and generating by the computer system a report based on the comparing, wherein the computer system comprises a computer processor and an electronic storage medium.