Patent ID: 6818418
Filing Date: 2004-11-16
Classification: C07K,C12N,C12Q,C40B

Abstract:
A method for obtaining a scaffold-based protein that binds to a compound, said method comprising:(a) contacting a compound with a library of scaffold-based proteins under conditions that allow binding to form a compound-scaffold-based protein complex, wherein the scaffold is derived from the tenth module of human fibronectin type III (10Fn3), said tenth module having the amino acid sequence, VSDVPRDLEVVAATPTSLLISWDAPAVTVRYYRITYGETGGNSPVQEFTVPGSKS TATISGLKPGVDYTITVYAVTGRGDSPASSKPISINYRT, said library comprising scaffold-based proteins having at least three randomized loops and being characterized by their ability to bind to a compound that is not bound by said human 10Fn3; and (b) obtaining, from said complex, a scaffold-based protein that binds to said compound and that has at least one amino acid alteration in each of three loops relative to the human 10Fn3 sequence.