Patent ID: 6806045
Filing Date: 2004-10-19
Classification: C12N,G01N

Abstract:
A method for the identification and investigation of a receptor in target tissue for which a selected vector has affinity, said method comprising:i) creating retroviral particles containing a library of mRNA from the targe tissue; ii) transfecting a non-adherent cell line which does not bind with the selected vector by infecting the cells with said retroviral particles; iii) adding to the transfected cell line a suspension of encapsulated gas microbubbles to which the selected vector is coupled and allowing the microbubbles and cells coupled thereto to float to the surface of the suspension; iv) isolating the microbubble-bound cells at the surface; and either v-a) lysing the isolated cells, amplifying the receptor-encoding cDNA therefrom and sequencing said cDNA; and optionally v-b) comparing the thus-obtained sequence data with gene bank sequence data; or vi-a) culturing the isolated cells; and vi-b) investigating affinities of vectors to the isolated cells.