Patent ID: 6743908
Filing Date: 2004-06-01
Classification: A61K,A61P,C07K,G01N

Abstract:
A polynucleotide encoding a single-chain antigen-binding polypeptide comprising:(a) a first polypeptide comprising an antigen-binding portion of a variable region of an antibody heavy or light chain; and (b) a second polypeptide comprising an antigen-binding portion of a variable region of an antibody heavy or light chain; and (c) a peptide linker linking the first and second polypeptides into a single chain polypeptide having an antigen-binding site, wherein the encoded single-chain antigen-binding polypeptide comprises at least one tripeptide comprising Asn-Xaa-Yaa, wherein Xaa is an amino acid other than proline and Yaa is threonine or seine, and the Asn residue of the tripeptide is located at a position selected from the group consisting of (i) amino acid position 11, 12, 13, 14 or 15 of the light chain variable region; (ii) amino acid position 77, 78 or 79 of the light chain variable region; (iii) amino acid position 11, 12, 13, 14 or 15 of the heavy chain variable region; (iv) amino acid position 82B, 82C or 83 of said heavy chain variable region; (v) any amino acid position of the peptide linker; (vi) an amino acid position that is adjacent to the C-terminus of the second polypeptide; and combinations thereof, and wherein the encoded single-chain antigen-binding polypeptide is capable of being glycosylated at the Asn residue of the tripeptide when expressed by a suitable host cell.