Patent ID: 7833757
Filing Date: 2010-11-16
Classification: C12Q

Abstract:
1. In an improved method for the non-invasive early detection of colon cancer or intestinal cancer precursor cells in a sample by means of mutational analysis of two genes of each of two different signaling pathways, where the genes for the first pathway are APC, and β-catenin, and the genes for the second pathway are K-ras and B-raf, the improvement comprising using selected parts of the two genes in each of the signaling pathways which are combined for the analysis whereby the selection of the gene parts are determined by the following primer sequences and characterized in that where the method comprises the following steps: collecting a stool sample, homogenizing the sample, obtaining DNA from the sample, performing an amplification reaction in the genes for APC, K-ras, β-catenin and B-raf, using the following primers for APC: SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4, SEQ ID NO. 5, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 15, SEQ ID NO. 16, the following primers for K-ras: SEQ ID NO. 11, SEQ ID NO. 12, the following primers for β-catenin: SEQ ID NO. 13, SEQ ID NO. 14, and the following primers for B-raf: SEQ ID NO. 17, SEQ ID NO. 18, wherein amplification products are formed, and performing a mutational analysis in the amplification products to determine the existence of mutations in the APC, K-ras, β-catenin and B-raf genes.