Patent ID: 6165793
Filing Date: 2000-12-26
Classification: C07K,C12N,C12Q,C12Y,C40B,Y10S

Abstract:
A method for producing a variant polynucleotide, comprising:(a) conducting a polynucleotide extension process under conditions resulting in incomplete extension of synthesized polynucleotides to produce a plurality of polynucleotides in various stages of synthesis wherein at least 20% of the amplified strands are incompletely extended;(b) denaturing the plurality of polynucleotides;(c) incubating the denatured plurality of polynucleotides with a polymerase under conditions which result in annealing of single-stranded polynucleotides at regions of complementarity between the single stranded polynucleotides and formation of variant double stranded polynucleotide chains;(d) repeating (b) and (c);(e) screening or selecting a plurality of variant polynucleotides or expression products thereof to identify a variant polynucleotide having a useful functional activity.