Patent ID: 8334110
Filing Date: 2012-12-18
Classification: G01N,Y10T

Abstract:
1. A method for detecting amygdalin in the blood plasma of an animal after administering a botanical composition comprising Strengthening Body Resistance and Dissipating Blood Stasis Botanical to the animal, comprising the steps of: (a) collecting the blood plasma from the animal after the administration of Strengthening Body Resistance and Dissipating Blood Stasis Botanical, wherein said botanical composition comprises (b) mixing the blood plasma with phosphoric acid with a concentration ranging from 2-5 mol/L at a blood plasma to phosphoric acid volume ratio of 1:2-3 (v/v); (c) applying the mixture of step (b) to a chromatography column, wherein the column is pre-activated by methanol and water prior to application of the mixture; (d) washing the column with water and 80-100% methanol; (e) eluting the column with 0.2-1% ammonia in methanol to obtain an eluent, (f) drying the eluent at 25-30° C.; (g) dissolving the dried eluent of step (f) in a mobile phase A solution to form a dissolved eluent, wherein the mobile phase A solution comprises water, acetonitrile and formic acid at a water/acetonitrile/formic acid ratio (v/v/v) of 95:5:0.1; and (h) separating and detecting amygdalin in the dissolved eluent by ultra performance liquid chromatography/mass spectrometry (UPLC-MS), wherein the UPLC separation conditions comprise separating amygdalin on a 2.1×100 mm, C