Patent ID: 6124109
Filing Date: 2000-09-26
Classification: C12Q,G01N,Y10S

Abstract:
A composition for qualitative and/or quantitative analysis of biological substances by amplified chemiluminescence comprising a) at least one ligand capable of coupling to the biological substance, b) at least one cyclic diacylated dihydrazide fused to an aromatic chemiluminescence reagent, c) an oxido reduction luminescence enzyme, d) at least one oxidizing agent specific to enzyme c) and e) at least one amplifier of luminescence of the formula ##STR13## wherein R.sup.0 is selected from the group consisting of ##STR14## and polyorganosiloxane, R.sup.1 is selected from the group consisting of alkyl of 1 to 10 carbon atoms, alkylcarboxyl of 2 to 10 carbon atoms, alkylamino and aminoalkyl of 1 to 10 carbon atoms, aryl, alkylaryl and aralkyl, R is selected from the group consisting of haloqen, alkyl of 1 to 30 carbon atoms, aryl of 6 to 30 carbon atoms, aralkyl of 7 to 30 carbon atoms, alkylaryl of 7 to 30 carbon atoms, ##STR15## --CH.dbd.--Z, ##STR16## and alkyl of 1 to 6 carbon atoms, wherein W is hydrogen or carboxyl and Z is carboxyl or 2,4-dinitrophenyl, Y is selected from the group consisting of --CH.sub.2, --O-- and --N.dbd.N-- and V is hydrogen or Y is selected from the group consisting of --O--, --S-- and --S--S and V is --OH, one of A and B is hydrogen and the other is halogen or A is hydrogen or halogen and R and B together form ##STR17## and X is hydrogen or halogen.