Patent ID: 6689743
Filing Date: 2004-02-10
Classification: A01N,A61K,C07K,C12N,C12R,Y02A

Abstract:
An isolated protein that is active against a coleopteran pest, wherein said protein is encoded by a polynucleotide that hybridizes with a 285-basepair probe, wherein said probe is obtainable by PCR amplification with the primer shown in SEQ ID NO: 2 and with the reverse primer shown in SEQ ID NO: 3 of cellular DNA from E. coli NM522 having Accession No. NRRL B-21018, and wherein hybridization occurs at 42Â° C. in 50% formamide, 5Ã— Denhardt's solution, 5Ã—SSPE, 0.1% SDS, with 100 &mgr;g/ml single stranded DNA; and wash occurs at room temperature in 2Ã—SSC and 0.1% SDS.