Patent ID: 6821737
Filing Date: 2004-11-23
Classification: C40B,G01N

Abstract:
A method for identifying a transcription factor modulator, the method comprising:treating a sample of cells with a test agent, the treated sample forming the test sample, providing a library of double stranded nucleic acid probes, the nucleic acid probes each comprising a recognition sequence varying within the library and the nucleic acid probes in the library being capable of binding to at least two activated transcription factors selected from the group consisting of AP1, AP-2, ARE, Brn-3, C/EBP, CBF, CDP, c-Myb, CREB, E2F-1, EFR, ERE, Ets, Ets-1/PEA3, FAST-1, GAS/ISRE, GATA, GRE, HNF-4, IRF-1, MEF-1, MEF-2, Myc-Max, NF-1, NFATc, NF-E1, NF-E2, NF .kappa.B, Oct-1, p53, Pax-5, Pbx1, Pit 1, PPAR, PRE, RAR, RAR (DR-5), SIE, Smad SBE, Smad3/4, SP1, SRE, Stat1, Stat3, Stat4, Stat4, Stat5, Stat6, TFIID, TR, TR(DR-4), USF1, VDR (DR-3), HSE, and MRE, contacting the test sample with the library of double stranded DNA nucleic acid probes under conditions where nucleic acid probe-transcription factor complexes are formed between the nucleic acid probes and activated transcription factors present in the test sample, isolating the nucleic acid probes from the nucleic acid probe-transcription factor complexes formed, contacting the isolated nucleic acid probes with an array of immobilizing hybridization probes under conditions suitable for hybridization of the strands of the different double stranded nucleic acid probes to the hybridization probes in the array, wherein identification of the nucleic acid probes bound to the array determines which of the activated transcription factors are present in the test sample, and comparing the activated known transcription factors present in the test sample with the activated known transcription factors present in a control sample of cells not contacted with the test agent, the difference in the presence of transcription factors between the test and control sample being indicative of the transcription modulation by the agent contacted with the test sample.