Patent ID: 8897536
Filing Date: 2014-11-25
Classification: G01N,G06K

Abstract:
1. A method for gel image analysis implemented in a medical system with one or more processors, said method comprising: (a) receiving the approximate coordinates of a first protein spot on a first gel image; (b) identifying the perimeter of said first protein spot using a wave search based on the pixel-intensity of the area surrounding said approximate coordinates; (c) identifying the center coordinates of said first protein spot using a Levenberg-Marquardt fitting algorithm to model pixel intensity within said perimeter of said first protein spot, said Levenberg-Marquardt fitting algorithm based on a Gaussian function; (d) locating a second protein spot on a second gel image using a molecular block-matching method, said second protein spot represents the same protein as said first protein spot, and said molecular block-matching method comprises: (e) identifying the perimeter of said second protein spot using a wave search based on the pixel-intensity of the area surrounding said approximate coordinates of said second protein spot; and (f) identifying the center coordinates of said second protein spot using a Levenberg-Marquardt fitting algorithm to model pixel intensity within said perimeter of said second protein spot, said Levenberg-Marquardt fitting algorithm based on a Gaussian function, whereby the magnitude and direction of displacement of said second protein spot relative to said first protein spot can be ascertained.