Patent ID: 7005414
Filing Date: 2006-02-28
Classification: C07K

Abstract:
1. Method for the production of artificial oxygen carriers comprising crosslinked hemoglobin, said method comprising the following steps: i) deoxygenizing a first solution comprising hemoglobin to form a second solution comprising deoxygenated hemoglobin; ii) covalently reacting the second solution comprising deoxygenated hemoglobin with a chemically reactive effector of an oxygen bonding to form a third solution; iii) treating the third solution with a non-chemically reactive effector of an oxygen bonding to form a fourth solution; and then iv) stably covalently crosslinking the hemoglobin with glutaraldehyde by diluting the fourth solution and adding a crosslinking agent, then further diluting the fourth solution, while the volume of the fourth solution increases overall by a factor of 2 to 10; and then v) covalently linking a polyethylene oxide derivative to the hemoglobin; and vi) processing and formulating a resulting product as a pharmaceutical.