Patent ID: 6875572
Filing Date: 2005-04-05
Classification: C12N,C12Q

Abstract:
1. A method for detecting the presence of a target nucleic acid molecule by detecting non-target cleavage products, comprising: a) providing: b) combining said cleavage agent, said target nucleic acid, said first oligonucleotide and said second oligonucleotide under reaction conditions such that at least said first portion of said first oligonucleotide is annealed to said first region of said target nucleic acid, and wherein at least said 5′ portion of said second oligonucleotide is annealed to said second region of said target nucleic acid so as to create a cleavage structure, and wherein cleavage of said cleavage structure occurs and cleaves said first oligonucleotide to generate a non-target cleavage product; and c) detecting the cleavage of said cleavage structure.