Patent ID: 6451588
Filing Date: 2002-09-17
Classification: C12Q

Abstract:
A first array of a plurality of single-stranded first probes and a second array of a plurality of single-stranded second probes, whereinthe first probes comprise a first portion complementary to a first region of a target nucleic acid and capable of hybridizing thereto, and a second portion capable of hybridizing with the second probe, wherein the first and second portions of the first probe are joined by a flexible linker, wherein the flexible linker comprises 1 to 6 ethyleneoxy units, alkyldiyl of 1 to 20 carbon atoms, or aryldiyl of 6 to 20 carbons atoms; and the second probes comprise a first portion complementary to a second region of the target nucleic acid and capable of hybridizing thereto, and a second portion capable of hybridizing with the first probe; the first region of the target nucleic acid and the second region of the target nucleic acid are substantially adjacent; each of the first and the second region of the target nucleic acid is from three to eight nucleotides in length; at least one of the first probe or the second probe comprises a high-affinity nucleic acid analog; at least one of the first probe or the second probe comprises a fluorescent detection moiety; the portion of each of the first probes complementary to the first region of the target nucleic acid has a different sequence, wherein the first region of the target nucleic acid is x nucleotides in length and x is greater than or equal to 6; the array of first probes comprises at least (0.5Ã—4x)Ã·N first probes, wherein N is equal to the degeneracy of each of the first portions of each of the first probes; and the portion of each of the second probes complementary to the second region of the target nucleic acid has a different sequence.