Patent ID: 6479264
Filing Date: 2002-11-12
Classification: C12N,C12Q

Abstract:
A method for reversibly inactivating thermostable DNA polymerase or ligase, which method comprises reacting a mixture of the thermostable DNA polymerase or ligase with a dicarboxylic acid anhydride, wherein the reaction is carried out using a dried DNA polymerase or ligase in an anhydrous aprotic organic solvent, the dicarboxylic acid anhydride being also substantially anhydrous, whereby the reaction results in essentially complete inactivation of enzyme activity, wherein the anhydrous aprotic organic solvent is selected from the group consisting of t-methyl butyl ether (t-MBE), butyl ether, carbon tetrachloride, cyclohexanone, ethyl acetate, methyl ethyl ketone, methyl pentanone, propyl ether, pyridine and sulfolane.