Patent ID: 8486371
Filing Date: 2013-07-16
Classification: G01N

Abstract:
1. A method of multi-dye analysis of particles using flow cytometer, said method comprising: labeling particles to be detected using two or more dyes, said two or more dyes comprising a trigger dye and a reporter dye, said trigger dye is selected from carboxyfluorescein succinimidyl ester (CFSE) and fluorescein isothiocyanate (FITC), said reporter dye is DeepRed; suspending said particles within a non-uniform flow in a capillary; exciting a first of said particles within said capillary using a multiphoton excitation laser beam causing said two or more dyes each to fluoresce thereby producing a first output signal and a second output signal respectively; detecting said first output signal and said second output signal; exciting a second of said particles within said capillary using said multiphoton excitation laser beam causing said two or more dyes each to fluoresce thereby producing a third output signal and a fourth output signal respectively; and comparing a ratio of said first output signal and said second output signal to a ratio of said third output signal and said fourth output signal to detect a desired change in said particles.