Patent ID: 9127242
Filing Date: 2015-09-08
Classification: C12M

Abstract:
1. A single-use bioreactor system for culturing of a tissue, an organ or a graft, comprising: a controlled environmental module configured to facilitate cell growth and to provide a sterile environment for seeding, operation, and/or harvesting of a finished organ or graft from the single-use bioreactor system; a disposable, flexible cell culture bag having a chamber and one or more injection ports; a biocompatible scaffold comprising a surface for the attachment of a plurality of cells thereto and growth of thereof, the biocompatible scaffold disposed within the chamber of the disposable cell culture bag; a motion system for cyclically moving the tissue, the organ, or the graft on the surface of the biocompatible scaffold; a disposable bioreactor in fluidic communication through a fluidic pathway with the chamber of the disposable cell culture bag, the disposable bioreactor comprising: a circulation pump positioned for producing a circulation of the fluidic media through the fluidic pathway between the disposable bioreactor and the chamber of the disposable cell culture bag of the bioreactor system, wherein the controlled environmental module is further configured to provide for inoculating starting cells through the one or more injection ports and onto the surface of the scaffold while the culture bag is disposed therein.