Patent ID: 6753173
Filing Date: 2004-06-22
Classification: C12N,C12P

Abstract:
A hybrid modular polyketide synthase (PKS) comprising at least a first extender module and a second extender module of a different PKS from said first module,wherein said extender modules are defined as consisting of the amino acid sequence from the N-terminus of the ketosynthase (KS) domain through the C-terminus of the acyl transferase protein (ACP) domain: wherein the C-terminus of said first module is covalently linked to the N-terminus of a intra-molecular linker (RAL) and the N-terminus of the second module is covalently linked to the C-terminus of said RAL, and wherein said RAL is defined as the amino acid sequence between the C-terminus of an upstream ACP domain and the N-terminus of an adjacent downstream KS domain; said ACP and KS domains occupying adjacent modules in the same reading frame; wherein either said first module or second module is not covalently linked to said RAL in a naturally occurring polyketide synthase; whereby the RAL effects the transfer of a nascent polyketide chain from said first module to said second module.