Patent ID: 8993844
Filing Date: 2015-03-31
Classification: C07K,C12N

Abstract:
1. A DNA construct wherein said DNA construct comprises: (a) a nucleic acid sequence comprising a plant endosperm tissue promoter; (b) wherein said plant endosperm tissue promoter is operably linked to a DNA sequence encoding a synthetic spider silk protein sequence, wherein said protein sequence is selected from the group consisting of SEQ ID NO: 20, SEQ ID NO: 21, SEQ ID NO: 22, SEQ ID N 23, SEQ ID NO: 24, SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, SEQ ID NO: 32, SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 36, SEQ ID NO: 38, and SEQ ID NO: 40; (c) wherein said DNA sequence encoding said spider silk protein sequence is operably linked to a DNA sequence encoding a regulatory region of a transcription terminator sequence, wherein said regulatory region of said transcription terminator sequence comprises SEQ ID NO: 5; and (d) wherein said regulatory region of said transcription terminator sequence is operably linked to a sortable marker selected from the group consisting of SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 47, SEQ ID NO: 49, SEQ ID NO: 51, and SEQ ID NO: 53.