Patent ID: 8263367
Filing Date: 2012-09-11
Classification: C12N,C12Q

Abstract:
1. A method of preparing at least one isolated linear composite nucleic acid molecule comprising a first tag from a first nucleic acid molecule and a second tag from a second nucleic acid molecule, wherein the first and second nucleic acid molecules interact in a nucleic acid-protein complex and wherein the first and second tags are from different nucleic acid molecules; comprising the steps of: (i) providing at least one nucleic acid-protein complex comprising at least one first nucleic acid molecule interacting with at least one second nucleic acid molecule; (ii) (a) ligating at least one linker with at least one non-ligatable end to at least one end of the nucleic acid molecule(s) in the nucleic acid mixture; (b) converting the non-ligatable end(s) of the ligated linker(s) to make them ligatable; and (c) ligating the linker(s) to ligate the end(s) of the nucleic acid molecules to form at least one linker-ligated linear composite nucleic acid molecule comprising at least one linker between the two nucleic acid molecules; (iii) size selecting for linker-ligated linear composite nucleic and molecules comprising a first tag from a first nucleic acid molecule; and a second tag from a second nucleic acid molecule; and (iv) ligating at least one adapter to at least one end of the linker-ligated linear composite nucleic acid molecule(s) and isolating at least one linear composite nucleic acid molecule with both adapter(s) and linker(s) ligated, wherein the first and second tags of the linear composite nucleic acid molecule(s) are from different nucleic and molecules.