Patent ID: 7214530
Filing Date: 2007-05-08
Classification: G01N,G03H

Abstract:
1. A method of making a biosensor, comprising the steps of: coating a photo-inactivating agent onto a surface of a substrate member; coating a receptive material layer over the photo-inactivating agent generally uniformly over the surface of the substrate member, the layer containing a receptive material specific for an analyte of interest; placing a mask over the substrate member, the mask having a configuration so as to shield at least one underlying area of the substrate member while exposing at least one adjacent area; irradiating the substrate member and mask combination with an energy source sufficient to chemically activate the photo-inactivating agent underlying the receptive material layer in the areas exposed by the mask, the chemically-activated photo-inactivating agent rendering the receptive material inactive in the exposed areas; removing the mask from the substrate member; and wherein a resulting pattern of active and inactive areas of the receptive material are defined, the inactive areas corresponding to the areas exposed by the mask and the active areas corresponding to the areas shielded by the mask such that when the biosensor is exposed to a medium containing the analyte of interest, the analyte binds to the receptive material in the active areas and subsequently facilitates diffraction of transmitted or reflected light in a diffraction pattern corresponding to the active areas.