Patent ID: 7110885
Filing Date: 2006-09-19
Classification: G16B

Abstract:
1. A method of processing gene sequence data with use of one or more computers, the method comprising: reading, by the computer, gene sequence data corresponding to a gene sequence and coding sequence data corresponding to a plurality of coding sequences within the gene sequence; identifying, by the computer following a set of primer selection rules, primer pair data within the gene sequence data, the primer pair data corresponding to a pair of primer sequences for one of the coding sequences, the set of primer selection rules including a first rule specifying that the primer pair data for the coding sequence be obtained for a predetermined annealing temperature; the set of primer selection rules including a second rule specifying that, based on a comparison of the primer pair data and gene family data, wherein the gene family data represents a gene family member of the gene sequence other than the gene sequence, stored in a file, the primer pair data for the coding sequence must fail to match the gene family data; storing the primer pair data; repeating the acts of identifying and storing such that primer pair data are obtained for each coding sequence of the plurality of coding sequences at the predetermined annealing temperature; and simultaneously amplifying the plurality of coding sequences in gene sequences from three or more individuals at the predetermined annealing temperature using the identified pairs of primer sequences, such that a plurality of amplified coding sequences from the three or more individuals are obtained.