Patent ID: 7959933
Filing Date: 2011-06-14
Classification: A61K,A61P,C07K,C12N,Y02A

Abstract:
1. A cleavable single-chain polypeptide comprising: a) a first amino acid sequence region comprising i) a first domain comprising a BoNT/A neurotoxin heavy chain binding element able to preferentially interact with a target cell surface marker under physiological conditions; and ii) a second domain comprising a BoNT/A neurotoxin heavy chain translocation element able to facilitate the transfer of said single-chain polypeptide across a vesicular membrane; and b) a second amino acid sequence region comprising a BoNT/A neurotoxin light chain or a portion thereof retaining a SNARE-protein sequence-specific endopeptidase activity; c) a third amino acid sequence region comprising a non-native Clostridial neurotoxin protease cleavage site; wherein said first and second amino acid sequence regions are separated by said third amino acid sequence region.