Patent ID: 6270981
Filing Date: 2001-08-07
Classification: G01N

Abstract:
A method of screening for a molecule capable of competing with OB-R for binding to Src homology 2 containing protein tyrosine phosphatase 2 (SHP-2) comprising:a) contacting an OB-R phosphopeptide comprising the Y986 domain of the OB-R of SEQ ID NO: 1 under conditions in which the OB-R phosphopeptide is capable of binding to an SHP-2-SH2 peptide comprising src homology domain 2 of SHP-2;b) determining the amount of the OB-R phosphopeptide that binds to the SHP-2-SH2 peptide;c) contacting a known amount of the OB-R phosphopeptide to the SHP-2-SH2 peptide, in the presence of a sample suspected of containing the molecule capable of competing with the OB-R phosphopeptide, under the same conditions used in b);d) determining the amount of the OB-R phosphopeptide that binds to the SHP-2-SH2 peptide; ande) comparing the amount from (b) with the amount from (d), wherein a lesser amount in (d) indicates the presence of a molecule capable of competing with the OB-R phosphopeptide for binding to the SHP-2-SH2 peptide.