Patent ID: 7777020
Filing Date: 2010-08-17
Classification: A61K,A61P,C07K,C12N,C12Q,G01N,Y10S

Abstract:
1. An envelope (env) nucleic acid product produced by the process comprising: (a) providing a sample comprising an env gene of HIV-1 nucleic acid; and (b) amplifying a template comprising sequence of the env gene of the HIV-1 nucleic acid from the sample using at least one pair of purified polynucleotides chosen from: (1) a polynucleotide from each of group (i) and group (ii), wherein group (i) comprises nucleotides 6905-6930, 7055-7077, 7360-7384, 7832-7857, 8844-8869, 7629-7647, 8224-8242 of the env gene of HIV-1 Bru; and wherein group (ii) comprises nucleotides 6930-6905, 7384-7360, 7857-7832, 8869-8844, and 8242-8224 of a nucleic acid sequence complementary to the env gene of HIV-1 Bru; (2) a polynucleotide from each of group (iii) and group (iv), wherein group (iii) comprises nucleotides 6903-6928, 7053-7075, 7349-7373, 7821-7846, 8836-8861, 7612-7630, and 8213-8231 of the env gene of HIV-1 Mal; and wherein group (iv) comprises nucleotides 6928-6903, 7373-7349, 7846-7821, 8861-8836, 8231-8213 of a nucleic acid sequence complementary to the env gene of HIV-1 Mal; and (3) a polynucleotide from each of group (v) and group (vi), wherein group (v) comprises nucleotides 6860-6885, 7010-7032, 7306-7330, 7775-7800, 8787-8812, 7572-7590, and 8167-8185 of the env gene of HIV-1 Eli; and wherein group (vi) comprises nucleotides 6885-6860, 7330-7306, 7800-7775, 8812-8787, and 8185-8167 of a nucleic acid sequence complementary the env gene of HIV-1 Eli, wherein the amplification is carried out under conditions that result in specific amplification of the env nucleic acid product from HIV-1 nucleic acid.