Patent ID: 8119356
Filing Date: 2012-02-21
Classification: G01N

Abstract:
1. A method of analyzing a sample to determine a level of a β1,6-N-acetylglucosamine-containing glycoprotein, the method comprising: providing a test agent comprising a first complex, wherein the first complex comprises a glycan bead and an phytohemagglutinin-L4 (L providing a sample comprising a β1,6-N-acetylglucosamine-containing glycoprotein; contacting the test agent with the sample to provide a test mixture, in which the β1,6-N-acetylglucosamine-containing glycoprotein binds with the first complex at the binding site, thereby forming a second complex in the mixture, wherein the second complex comprises the glycan bead, the L heating and applying a denaturing agent to the second complex to provide a denatured mixture, in which the β1,6-N-acetylglucosamine-containing glycoprotein is disassociated from the L separating the glycan bead from the denatured mixture to provide the β1,6-N-acetylglucosamine-containing glycoprotein substantially free of the glycan bead; subsequently fragmentizing the β1,6-N-acetylglucosamine-containing glycoprotein into smaller fragments; and analyzing the fragments using a mass spectrometric procedure to determine a level of the β1,6-N-acetylglucosamine-containing glycoprotein in the sample.