Patent ID: 8969088
Filing Date: 2015-03-03
Classification: G01N,Y10T

Abstract:
1. An in vitro method for evaluating a potency of a surfactant for inducing skin roughening, comprising: adding a surfactant to an engineered substrate model mimicking intercellular lipids in stratum corneum, the engineered substrate model consisting of a non-biological substrate, and a lipid membrane formed on the substrate so as to have a lamellar structure, the lipid membrane comprising ceramides, palmitic acid, and cholesterol; visualizing for a myelin figure on the engineered substrate by microscope, and; judging: the surfactant as causing skin roughening in vivo when the myelin figure is formed on the engineered substrate, and wherein the surfactant is more potent for inducing skin roughening when the shortest distance between the tip of the myelin figure and an edge of the engineered substrate is longer.