Patent ID: 6746837
Filing Date: 2004-06-08
Classification: A61K,C07K,Y10S

Abstract:
A method for determining the fungal multiple drug resistance (MDR) inhibition activity of a compound which comprises:a) placing a culture of fungal cells, transformed with a vector which expresses a nucleic acid encoding an atrD protein consisting essentially of the amino acid sequence of SEQ ID NO:2, in the presence of: (i) an antifungal agent to which said fungal cell is resistant, but to which said fungal cell is sensitive in its untransformed state; (ii) a compound suspected of possessing Aspergillus nidulans MDR inhibition activity; and b) determining the fungal MDR inhibition activity of said compound by measuring the ability of the antifungal agent to inhibit the growth of said fungal cell.