Patent ID: 7201901
Filing Date: 2007-04-10
Classification: A23J,A23L,A61L,C07K

Abstract:
1. A method of removing prion protein from a aqueous pharmaceutical composition comprising an IgG anti-D immunoglobulin, wherein the pharmaceutical composition comprises from about 4.0 to 6.0% immunoglobulin by weight, and from about 80 to 200 ppm polysorbate 80, comprising: (a) admixing the aqueous composition with from about 2% to about 10% methanol; and (b) filtering the admixture of step (a) through a depth filter having a pore size providing a retention of less than about 0.6 microns, thereby removing the prion protein, wherein the recovery of the biological protein in its original biological state is substantially maintained at least to a level in excess of about 50%, and wherein the removal of the abnormal infective prion protein may be achieved to an extent of at least about 10