Patent ID: 7560287
Filing Date: 2009-07-14
Classification: C07K,Y10S,Y10T

Abstract:
1. A method for determining whether a sample contains a target, comprising the steps of: A) contacting the sample with a probe coupled to a functional engineered fluorescent protein, the functional engineered fluorescent protein comprising an amino acid sequence which has at least 85% sequence identity to the amino acid sequence of SEQ ID NO:2 and which differs from SEQ ID NO:2 by at least i. an amino acid substitution T203X, wherein X is an aromatic amino acid selected from the group consisting of H, Y, W and F at a position corresponding to position 203 of SEQ ID NO:2, and ii. a substitution at position 65 in SEQ ID NO:2, wherein the substitution is selected from S65G, S65T, S65A, S65L, S65C, S65V or S65I; and wherein said functional engineered fluorescent protein has a different fluorescent property than B) determining whether the target has bound to the probe.