Patent ID: 9040052
Filing Date: 2015-05-26
Classification: A61K,C07K,C12Q

Abstract:
1. A method of reducing cholesterol level or maintaining previously reduced cholesterol level in a human in need thereof, the method comprising administering to said human an antibody or antibody fragment that specifically binds a proprotein convertase subtilisin/kexin type 9 (PCSK9) that comprises a C-terminal domain comprising a mutation selected from the group consisting of I474V and E670G in SEQ ID NO: 1, wherein the antibody or antibody fragment comprises a VH domain derived from the recombination of a human VH segment, a human D gene segment and a human JH segment, the human VH segment encoding the framework 1 of SEQ ID NO: 40, and wherein said human comprises: (i) a VH gene segment encoding the framework 1 of SEQ ID NO: 40, or the human expresses antibodies comprising VH domains derived from the recombination of a human VH segment, a human D gene segment and a human JH segment, the human VH segment encoding the framework 1 of SEQ ID NO: 40; and (ii) a nucleotide sequence encoding said PCSK9 that comprises a C-terminal domain comprising said selected mutation in SEQ ID NO: 1.