Patent ID: 7354735
Filing Date: 2008-04-08
Classification: C12Q,G01N

Abstract:
1. An isolated nucleic acid encoding a recombinant Aequorea victoria fluorescent protein that detects apoptosis, said fluorescent protein comprising: a) a donor fluorescent protein; b) an acceptor fluorescent protein; c) a peptide linker connecting said donor fluorescent protein to said acceptor fluorescent protein, wherein said peptide linker contains a cleavage site of a caspase, said peptide linker comprises SEQ ID NO: 23, and said cleavage site is flanked on each side with at least one glycine pair wherein XXXX represents a sequence selected from the group consisting of SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17; SEQ ID NO: 18, and SEQ ID NO: 19.