Patent ID: 7371745
Filing Date: 2008-05-13
Classification: C07D,C12Q,G01N

Abstract:
1. A method for detecting one or more molecules that include a target sequence, wherein said target sequence comprises an amino acid sequence of the form (H) i , wherein H is histidine and i is an integer of from 4 to 12, said method comprising: (a) providing a sample that comprises one or more target material, wherein said target material includes molecules having a target sequence, (b) subjecting said target material to electrophoresis in an electrophoretic medium; (c) contacting said electrophoretic medium with at least one molecule having the general structural Formula (I), and tautomers, salts and acids thereof: wherein (a) Y and Y′ are each a transition metal; (b) R wherein the contacting is under conditions sufficient to permit transition-metal-chelate moieties of said molecule of Formula (I) to associate with said target sequence, and wherein the electrophoretic medium is contacted with the at least one molecule of Formula (I) in a solution having an alkaline pH; and (d) detecting said detectable group of the molecule of Formula (I), thereby detecting said one or more molecules that include a target sequence.