Patent ID: 6593103
Filing Date: 2003-07-15
Classification: C07K,C12N,C12P

Abstract:
A cell-free system for translation and assembly of an HIV capsid, comprising:a cell-free translation mixture which contains a cell-free extract comprising a eukaryotic cell component of a high speed pellet, amino acids, transfer RNA (tRNA), ribosomes, and an energy source, an mRNA molecule encoding a Gag Pr55 protein derived from human immunodeficiency virus (HIV), and myristoyl coenzyme A, wherein said eukaryotic cell component is characterized by insensitivity to a concentration of at least 0.5% (wt/vol) octaethyleneglycol mono n-dodecyl ether detergent, wherein said eukaryotic cell component comprises a host assembly protein which is a 68 kilodalton that is present in a detergent treated high speed pellet of a wheat germ extract or a protein having the structure of said 68 kilodalton protein, and wherein said protein provides for capsid assembly in said cell-free system when said HIV Pr55 mRNA Gag protein is wild type.