Patent ID: 7604980
Filing Date: 2009-10-20
Classification: C07K,C12P

Abstract:
1. A novel expression cassette comprising an oligonucleotide encoding for a cleavage recognition site by a caspase, operably linked to a sequence encoding for a fusion protein, said fusion protein comprising a protein of interest having no internal cleavage site for caspase optionally fused to a purification tag, wherein the oligonucleotide is SEQ ID NO:13 or SEQ ID NO:14 encoding the caspase recognition site V-E-M-D (SEQ ID NO: 1) i.e. Valine-glutamic acid-methionine-aspartic acid (SEQ ID NO:1) or val-glu-met-asp (SEQ ID NO: 1) or V-E-I-D (SEQ ID NO: 2), and wherein the protein of interest is selected from the group consisting of SATB1, p53, PARP, Ikaros, cMyb, cMyc and HDAC1.