Patent ID: 6709825
Filing Date: 2004-03-23
Classification: B01J,B82B,B82Y,C07B,C07H,C12Q,C40B,Y10S

Abstract:
A method of detecting for the presence or absence of one or more nucleic acid targets in a sample, said method comprising:providing a satellite probe comprising (i) a type of particle having one or more types of oligonucleotides attached thereto, each type of oligonucleotides having a sequence wherein at least a portion of the sequence of the oligonucleotides is complementary to at least a portion of a sequence of a specific nucleic acid target; (ii) one or more types of probe oligonucleotides hybridized to the oligonucleotides attached to the particles, each type of probe oligonucleotides having a sequence that is identical to at least a portion of a sequence of a specific nucleic acid target; (iii) at least one reporter molecule attached to each probe oligonucleotide; and contacting the satellite probe with the sample under conditions effective to allow the probe oligonucleotide, in the presence of a nucleic acid target, to be displaced from the satellite probe by the nucleic acid target through a specific hybridization event, wherein hybridization overlap between the oligonucleotides attached to the particle and the nucleic acid target is greater relative to hybridization overlap between the probe oligonucleotides and the nucleic acid target; separating the particle; and detecting the reporter molecule.