Patent ID: 6716973
Filing Date: 2004-04-06
Classification: C07K,C12N

Abstract:
A polypeptide-engineered mRNA conjugate comprising:(a) an engineered mRNA which comprises: (1) ribozyme RNA which specifically covalently links to a peptide in the presence of the remainder of the ribozyme, wherein the ribozyme is the ribozyme of SEQ ID NO: 9; (2) a coding region for the peptide with which the ribozyme RNA of (a) covalently links, wherein the peptide is referred to as a peptide tag: (3) a coding region for a diverse polypeptide; and (4) two PCR primer-binding sites; and (b) the translation product of the engineered mRNA, which comprises the diverse polypeptide and the peptide tag, wherein the ribozyme RNA of the engineered mRNA is joined to the peptide tag by a covalent bond between the ribozyme RNA of the engineered mRNA and a reactive group of the peptide tag.