Patent ID: 7105294
Filing Date: 2006-09-12
Classification: C12Q

Abstract:
1. A method of determining the presence or absence of chromosomal aberrations in a sample of eukaryotic origin comprising the steps of: a) contacting said sample with a hybridization solution comprising at least two sets of hybridization probes, at least one first set comprising one or more probes capable of hybridizing to specific nucleic acid sequences flanking one side of a potential breakpoint in a chromosome, and at least one second set comprising one or more probes capable of hybridizing to specific nucleic acid sequences flanking the other side of the potential breakpoint, wherein the first set of probes is labeled with at least one first specific label while the second set of probes is labeled with at least one second specific label different from the first specific label, and wherein the two sets of hybridization probes hybridize such that the first and second specific labels produce a fused signal if no chromosome break is present; b) removing any unbound and any non-specifically bound probe; and c) detecting the hybridized probes, and thereby determining the presence or absence of chromosomal aberrations in the sample.