Patent ID: 6251644
Filing Date: 2001-06-26
Classification: A61K,A61L,C07K,C12N,C12Y

Abstract:
A method of inactivating non-lipid-enveloped viral contamination in a biological solution or suspension said solution or suspension, comprising a desired physiologically active component, comprising the steps of:a. mixing said biological solution or suspension with a photosensitizer that binds to said non-lipid-enveloped viral contaminant to form a mixture, wherein said photosensitizer has the formula: ##STR12##wherein .mu. is an integer from 1 to 6; X is an anionic counterion; R.sub.1, R.sub.2, R.sub.3, R.sub.4, R.sub.5, and R.sub.6 are independently a halogen; H; linear or branched alkyl of 1-10 carbon atoms; linear or branched alkoxy of 1-10 carbon atoms; --(CH.sub.2).sub.m --O--(CH.sub.2).sub.p --Z.sup..sym. R'R""R'"" or --O--(CH.sub.2).sub.n --Z.sup..sym. R'R""R'"" wherin n, m and p are independently integers from 1 to 10, Z is N or P, and R', R"" and R'"" are independently H or linear or branched alkyl of 1 to 10 carbon atoms with the proviso that on each Z atom, not more than two of R', R"" and R'"" may be H; and at least one of R.sub.1, R.sub.2, R.sub.3, R.sub.4, R.sub.5, or R.sub.6 is --(CH.sub.2).sub.m --O--(CH.sub.2).sub.p --Z.sup..sym. R'R""R'"" or --O--(CH.sub.2).sub.n --Z.sup..sym. R'R""R'"", or a halogen; and wherein said photosensitizer inactivates said non-lipid-enveloped viral contaminant upon absorption of radiation;b. adjusting an operating condition of said mixture from step (a) in order to increase the permeability of said non-lipid-enveloped viral contaminants to said photosensitizer; andc. irradiating said mixture from step (b) with ultraviolet, visible or ionizing radiation under conditions whereby substantially all of said non-lipid-enveloped viral contaminants are inactvated and the physiological activity of said component is substantially unimpaired.