Patent ID: 7727756
Filing Date: 2010-06-01
Classification: C07K,C12N

Abstract:
1. A variant of a JP170 type subtilase, said variant having at least 95% identity to SEQ ID NO:1 and comprising at least one amino acid sequence modification in at least one position of an ion-binding site 1, an ion-binding site 2, or an ion-binding site 3, or combinations thereof, wherein the at least one position of ion-binding site 1 is selected from the group of positions consisting of 183, 184, 185, 186, 187, 188, 189, 191, 196, 197, 198, 199, 200, 201, 202, 203, 224, 225, and combinations thereof; wherein the at least one position of ion-binding site 2 is selected from the group of positions consisting of 378, 379, 380, 381, 382, 383, 384, 385, 386, 387, 388, 389, 390, 391, 392, 393, and combinations thereof; and wherein the at least one position of ion-binding site 3 is selected from the group of positions consisting of 348, 350, 352, 363, 364, 365, 366, 367, 370, 394, 395, 396, 397, 398, 399, 400, 414, 415, 416, 417, 418, 419, 420, and combinations thereof; and wherein the numbering of said positions corresponds to the amino acid positions of SEQ ID NO:1.