Patent ID: 8845879
Filing Date: 2014-09-30
Classification: G01N

Abstract:
1. A method for monitoring the metabolic state of an organelle comprising the steps of: electrically coupling an organelle-modified bio-electrode to an electrode having opposite polarity by contacting an aqueous carrier between the bio-electrode and the electrode having opposite polarity where said aqueous carrier includes an electrolyte and reacting an effective amount of an organelle substrate at the bio-electrode for releasing a ionic product in said aqueous carrier thereby providing a current to the electrode having opposite polarity; capturing metabolic flux data during a reaction when an organelle modulating agent is present in the aqueous carrier and capturing control metabolic flux data when the organelle modulating agent is not present, comparing the metabolic flux data with the control metabolic flux data and determining a metabolic state of the organelle when the organelle modulating agent is present; and determining organelle metabolic activity as a function of metabolic substrate to determine modulating agent toxicity.