Patent ID: 7662576
Filing Date: 2010-02-16
Classification: G01N

Abstract:
1. A method for identifying an ester cooler comprising contacting a Transient Receptor Potential Cation Channel, Subfamily A, Member 1 (TRPA1) protein in a cell-based assay, with an agent to be tested for potential activity as an ester cooler; determining whether the agent increases or decreases the activity of the TRPA1 protein; selecting for the agent that increases the activity of the TRPA1 protein; determining the cooling strength of said agent that increases the activity of the TRPA1 protein; and organoleptically comparing the cooling strength of said agent that increases the activity of the TRPA1 protein relative to the cooling strength of menthol, whereby an increase in the cooling strength of said agent relative to the cooling strength of menthol thereby identifies said test agent as an ester cooler.