Patent ID: 6686155
Filing Date: 2004-02-03
Classification: A61K,A61P,C12N,C12Q,C12Y

Abstract:
A method for determining a platinum-based chemotherapeutic regimen for treating a tumor in a patient comprising:(a) obtaining a tissue sample of the tumor and fixing the sample, to obtain a fixed tumor sample; (b) isolating mRNA from the fixed tumor sample; (c) subjecting the mRNA to amplification using a pair of oligonucleotide primers capable of amplifying a region of the GST-pi gene, to obtain an amplified sample; (d) determining the amount of GST-pi mRNA in the amplified sample; (e) comparing the amount of GST-pi mRNA from step (d) to an amount of mRNA of an internal control gene to obtain a relative GST-pi expression level; and (f) determining a platinum-based chemotherapeutic regimen based on the relative GST-pi expression level in the amplified sample and a predetermined threshold level for GST-pi gene expression.