Patent ID: 6017769
Filing Date: 2000-01-25
Classification: G01N,Y10S

Abstract:
A method for producing chemiluminescence comprising:a) contacting an acid with a compound of formula I ##STR45## wherein Z.sup.1 and Z.sup.2 are independently selected from O, S and NR.sup.12, R.sup.12 is selected from alkyl, aryl, alkylsulfonyl and arylsulfonyl groups, R.sup.1 is a group containing from 1 to about 50 non-hydrogen atoms selected from C, N, O, S, P, Si and halogen atoms which is removable by an acid and R.sup.2 and R.sup.3 are organic groups containing from 1 to 50 non-hydrogen atoms selected from C, N, O, S, P, Si and halogen atoms, R.sup.4 -R.sup.11 are independently selected from hydrogen and substituents which do not interfere with the generation of chemiluminescence, to form a first reaction product; andb) contacting the first reaction product with a sufficient quantity of a base to provide a basic environment, at least one of the steps including providing an oxidant for reaction with either compound I or the first reaction product, wherein a second reaction product is formed and the light is produced in the basic environment.