Patent ID: 6146511
Filing Date: 2000-11-14
Classification: C12N,G01N

Abstract:
A method for purifying a nucleic acid sample comprising the steps of:providing a nucleic acid sample comprising a desired nucleic acid and one or more contaminants;providing an electrophoresis matrix having a loading/recovery well formed therein;placing the nucleic acid sample into the loading/recovery well;performing a first electrophoresis comprising electrophoresing the nucleic acid sample for a first time effective to transport the desired nucleic acid out of the loading/recovery well and into the electrophoresis matrix; andperforming a second electrophoresis comprising electrophoresing the nucleic acid sample for a second time effective to transport the desired nucleic acid out of the electrophoresis matrix and back into the loading/recovery well;wherein the first and second electrophoresis is effective to substantially reduce the concentration of contaminants relative the concentration of desired nucleic acid, thereby producing a purified nucleic acid.