Patent ID: 8202728
Filing Date: 2012-06-19
Classification: C12N,Y10T

Abstract:
1. A method for coating a multi-well substrate having a plurality of wells with crystals comprising calcium phosphate, comprising: (a) introducing into the wells of the substrate solutions comprising a plurality of precursor components of the crystals comprising calcium phosphate in concentrations for producing the crystals wherein each of the solutions has initial pH from 5 to less than 7.0; (b) inverting the substrate, wherein the solutions remain at the bottom of the wells of the inverted substrate due to the surface tensions of the solutions in the wells of the inverted substrate; and (c) incubating the inverted substrate for a time at an appropriate temperature so that the crystals comprising calcium phosphate are produced and coated on the surface of at least one well of the wells of the substrate and a gas generated by the reactions of the plurality of precursor components in the solutions during the incubation is released out from the wells.