Patent ID: 6174686
Filing Date: 2001-01-16
Classification: G01N,Y10S

Abstract:
An assay for determining the presence or amount of all free cardiac specific troponin isoforms and complexed isoforms of cardiac troponin in a blood sample, said complexed isoforms comprising isoforms selected from the group consisting of troponin I, troponin C and troponin T, wherein said assay comprises:contacting said sample with at least one inhibitor, wherein said inhibitor causes dissociation of said complexed isoforms; andperforming an immunoassay with antibody specific to cardiac troponin, wherein said antibody binds said free isoforms in said sample,wherein a detectable signal derived from said immunoassay is related to the presence or amount of said free and said complexed cardiac specific troponin isoforms in said sample.