Patent ID: 8652780
Filing Date: 2014-02-18
Classification: C12Q

Abstract:
1. A method for detecting the presence or absence of a plurality of target alleles at a plurality of polymorphic loci in a sample, wherein the sample contains maternal nucleic acid and fetal nucleic acid, which comprises: a) cleaving nucleic acid comprising a plurality of non-target alleles at the plurality of polymorphic loci with a single cleavage agent that recognizes the non-target alleles at single-nucleotide polymorphic loci, wherein the cleavage agent is a non-thermostable restriction endonuclease; b) elevating the temperature to a temperature that inactivates the endonuclease; c) after (b), amplifying uncleaved nucleic acid but not cleaved nucleic acid; and d) analyzing the amplification products of step (c) to determine the presence or absence of a plurality of target alleles, wherein