Patent ID: 8592155
Filing Date: 2013-11-26
Classification: C12Q

Abstract:
1. A method for identifying a chromosomal deletion, comprising the steps of: hybridizing a first probe set to a metaphase chromosome spread or an interphase nucleus, said first probe set being specific for-a deletion region of a chromosome but less than an entire chromosome such that said first probe set hybridizes to the deletion region if it is present within the metaphase chromosome spread or the interphase nucleus, and does not substantially hybridize to any region if the deletion region is absent from the metaphase chromosome spread or interphase nucleus, and hybridizing a second probe set to the metaphase chromosome spread or an interphase nucleus, said second probe set being specific for nucleotides in a region of the chromosome flanking the deletion region but less than an entire chromosome such that said second probe set will hybridize to the flanking region regardless of whether the deletion region is present or absent within the metaphase chromosome spread or interphase nucleus; wherein (a) said first and second probe sets are distinct from one another and are each detectably labeled, (b) the label for the first probe set is distinguishable from the label for the second probe set and (c) each of the first and second probes has a complexity of at least 50,000 bases.