Patent ID: 6306575
Filing Date: 2001-10-23
Classification: A61K,C07K,C12N

Abstract:
A negative selection process for enriching and recovering human hematopoietic progenitor cells and human hematopoietic stem cells in a sample containing human hematopoietic differentiated cells, human hematopoietic progenitor cells, human hematopoietic stem cells, and tumor cells comprising:(a) reacting the sample with an antibody composition containing antibodies capable of binding to the antigens glycophorin A, CD3, CD24, CD16, and CD14, and containing an antibody specific for a non-hematopoietic antigen expressed on the tumor cells, under conditions so that antibody-cell conjugates are formed between the antibodies and cells in the sample containing the antigens glycophorin A, CD3, CD24, CD16, and CD14 and non-hematopoietic antigens on their surface;(b) removing the conjugates; and,(c) recovering a cell preparation which is enriched in human hematopoietic progenitor cells and human hematopoietic stem cells, wherein the cell preparation recovered in step (c) has about a 3 to 5 log depletion of tumor cells and differentiated cells and is not labeled or coated with antibodies.