Patent ID: 7368232
Filing Date: 2008-05-06
Classification: A61P,C07K,C12N,C12Q,G01N,Y10S

Abstract:
1. A method of detecting virus binding and entry to a target cell, said method comprising the steps of: attaching an enzyme to the C-terminal end of a viral envelope protein, wherein the C-terminal end of the viral envelope protein is located on the inner side of the viral membrane, thereby creating an envelope-enzyme fusion protein; generating virus particles comprising said fusion protein and wild type viral envelope protein, wherein the enzyme of the envelope-enzyme fusion protein is encapsidated in the virus particles; infecting target cells with the virus particles; and measuring activities of the enzyme in infected cells, wherein the activities measured are the activities of the enzyme incorporated into the virus itself, wherein levels of enzyme activities are measures of virus binding and entry to the target cells mediated by the wild type envelope protein.