Patent ID: 9068170
Filing Date: 2015-06-30
Classification: A61P,C12N

Abstract:
1. A method of producing an induced pluripotent stem cell from a mammalian non-pluripotent cell, the method comprising: contacting the non-pluripotent cell with a plurality of exogenous transcription factor polypeptides, each exogenous transcription factor polypeptide comprising a transcription factor fused to a heterologous peptide sequence that enhances transport across cell membranes, under conditions to produce the induced pluripotent stem cell from the mammalian non-pluripotent cell; wherein the exogenous transcription factor polypeptides are selected from the group consisting of: (i) Oct4, Klf4, and Sox2; and (ii) Oct4, Klf4, Sox2, and c-Myc; and contacting the non-pluripotent cell with a MEK inhibitor, a GSK3 inhibitor, and a TGFβ receptor inhibitor/ALK5 inhibitor.