Patent ID: 8697442
Filing Date: 2014-04-15
Classification: A61L,A61P,C12N

Abstract:
1. A method to repair cartilage in a subject by implantation of chondrocytes comprising (a) subjecting a cartilage tissue sample from said subject to a single digestion enzyme or to more than one digestion enzyme sequentially or simultaneously, wherein the total period of time of exposure to the single digestion enzyme or to said more than one digestion enzymes is at least ten minutes and less than 2 hours to release said chondrocytes, (b) isolating the chondrocytes released in step (a) by separating the chondrocytes from the digestion enzyme or enzymes, thereby effectively ending the digestion process to obtain isolated chondrocytes, and directly implanting the chondrocytes into said subject; wherein said method employs step (a) as the only treatment of said sample with any digestion enzyme.