Patent ID: 8084252
Filing Date: 2011-12-27
Classification: C12N

Abstract:
1. A method for preparing a recombinant polypeptide in a cell culture, comprising: multiplying an isolated stable mammalian recombinant cell clone that has a selection marker and contains a sequence encoding the recombinant polypeptide in a serum- and protein-free medium comprising a mixture of amino acids containing L-asparagine, L-cysteine, L-cystine, L-proline, L-tryptophan and L-glutamine, wherein the stable cell clone is cultured with neither selective pressure for the selection marker nor selection for a polypeptide factor that replaces serum components, preparing a cell culture containing the stable cell clone in a bioreactor, and harvesting the recombinant polypeptide from the culture supernatant.