Patent ID: 8637092
Filing Date: 2014-01-28
Classification: A61K,A61P

Abstract:
1. A method for preparing a pharmaceutical composition for treatment of diabetes mellitus, wherein the pharmaceutical composition comprises extracts of the following crude drugs: 5-40 weight parts of Radix Trichosanthis , 10-30 weight parts of Radix Bupleuri , 3-15 weight parts of Fructus Aurantii Immaturus , 1-6 weight parts of Radix et Rhizoma Rhei , 1-12 weight parts of Rhizoma Pinelliae , 3-15 weight parts of Radix Scutellariae , 1-12 weight parts of Rhizoma Coptidis , 3-15 weight parts of Radix Paeoniae Alba and 5-20 weight parts of Fructus Mume , and optionally comprises pharmaceutically acceptable excipient; wherein the pharmaceutical composition is prepared by any one of the following four methods: The first method comprising the following steps: providing the crude drugs in accordance with the weight parts as described above; extracting the crude drugs with water by reflux for at least one time to obtain at least one aqueous extract; collecting and filtering the at least one aqueous extract and concentrating under reduced pressure to obtain a concentrated extract; adding ethanol into the concentrated extract to make the ethanol content to 65-75%; filtering the concentrated extract to obtain a filtrate; concentrating the filtrate under reduced pressure to produce an extract; and optionally adding an excipient into the extract of the extract derived from concentrating the filtrate to prepare into a pharmaceutically acceptable dosage form; The second method comprising the following steps: providing the crude drugs in accordance with weight parts as described above; extracting the crude drugs with water by reflux for at least one time to obtain at least one aqueous extract; collecting and cooling down the at least one aqueous extract, filtering to give a filtrate; loading the filtrate onto macro-porous adsorption resin, washing with water to obtain an eluate, and discarding the eluate; continuing to wash the resin with ethanol having a certain concentration for at least one time to obtain at least one ethanolic eluate; collecting the at least one ethanolic eluate and recovering ethanol under reduced pressure to give an extract; and optionally adding an excipient into the extract obtained in the previous step to prepare into a pharmaceutically acceptable dosage form; The third method comprising the following steps: providing the crude drugs in accordance with weight parts as described above; extracting the following crude drugs with ethanol having a certain concentration by reflux to obtain an extraction residue and an ethanolic extract of: adding the combining the filtrate and concentrating into an extract; optionally adding an excipient to the extract to prepare into a pharmaceutically acceptable dosage form; The fourth method comprising the following steps: providing the crude drugs in accordance with weight parts as described above; extracting the extracting the extracting the combining the dry powder extract obtained in the above three steps, into which an excipient is optionally added to prepare into a pharmaceutically acceptable dosage form.