Patent ID: 6468752
Filing Date: 2002-10-22
Classification: C12Q,G01N

Abstract:
A method for the detection of chemical substances, comprising:providing an agent comprising a first polynucleotide or peptide sequence having a first fluorophoric molecule, wherein first end of said first polynucleotide or peptide sequence is bonded to a solid phase, and a second polynucleotide or peptide sequence having a second fluorophoric molecule, wherein a second end of said second polynucleotide or peptide sequence has a group which is sensitive to the chemical substance to be detected, said chemical substance comprising a physical property sensitive to an external force, the first polynucleotide or peptide sequence being hybridizable to the second polynucleotide or peptide sequence such that a spatial relationship making possible an interaction between the first and the second fluorophoric molecule is producible, and the spatial relationship being terminatable and thus a fluorescence reaction being producible upon addition of the chemical substance to the group and action of an external force; contacting the agent with the chemical substance to be detected; applying the external force; and detecting the presence or absence of the fluorescence reaction.