Patent ID: 7635666
Filing Date: 2009-12-22
Classification: B01J,C07K,C12N,C40B,G01N,Y10S

Abstract:
1. A method of obtaining a member of a specific binding pair, the method comprising: providing a library of in vitro mutagenized nucleic acid molecules from an existing antibody encoding nucleic acid molecule, producing a library of filamentous bacteriophage particles displaying on the surface a population of specific binding pair members which comprise a binding domain of an immunoglobulin, each filamentous bacteriophage particle containing a nucleic acid molecule from the library of in vitro mutagenized nucleic acid molecules from an existing antibody encoding nucleic acid molecule, contacting the library of filamentous bacteriophage particles with a desired epitope, and separating filamentous bacteriophage particles displaying on the surface specific binding pair members comprising a binding domain which binds to said epitope, wherein the specific binding pair members are Fabs.