Patent ID: 6444878
Filing Date: 2002-09-03
Classification: C12N

Abstract:
A selection method for selecting genetically transformed plant cells from a population of plant cells, comprising:transforming a population of plant cells with an expressible nucleotide sequence having at least 75% identity to SEQ ID NO: 1 and which encodes an enzyme having glucosamine-6-phosphate deaminase activity that converts a component or a metabolic derivative thereof when present in a high concentration in a medium into a nutrient, thereby producing a population of genetically transformed and non-transformed cells; introducing said population of plant cells to a medium comprising said component or metabolic derivative thereof, wherein the component or the metabolic derivative thereof is present in said medium in a high concentration and is toxic to said non-transformed plant cells and is a source of both carbohydrate and nitrogen for the selectable, genetically transformed plant cells or if a portion of the component serves as a metabolic substrate and is metabolically converted to a derivatized substrate, then that derivatized substrate provides an allosteric effect on the gene product; and culturing said plant cells thereby selecting at least a portion of said transformed plant cells over said non-transformed plant cells; and, wherein said enzyme having glucosamine-6-phosphate deaminase activity converts glucosamine-6-phosphate to fructose-6-phosphate.