Patent ID: 8623603
Filing Date: 2014-01-07
Classification: C12Q

Abstract:
1. A method for enriching a target sequence in an amplification mixture, said method comprising: a) preparing an amplification reaction mixture comprising at least the following constituents: b) increasing the temperature of the reaction mixture suspected of having said target sequence to a first denaturing temperature that is above the melting temperature (T c) reducing the temperature of the reaction mixture so as to permit formation of duplexes of the reference blocking sequence and the complementary reference strand and heteroduplexes of the reference blocking sequence and target strands; d) increasing the temperature of said reaction mixture to a critical temperature (T e) reducing the temperature of the reaction mixture so as to permit said primer pair to anneal to denatured target strands and any denatured reference strands in the reaction mixture; f) extending said primers annealed to the denatured target strands and denatured reference strands in the reaction mixture; and g) repeating steps b) through f) in order for two or more cycles so as to enrich said target sequence relative to said reference sequence.