Patent ID: 7470511
Filing Date: 2008-12-30
Classification: C12Q

Abstract:
1. A method for detecting a likelihood of cancer, comprising: (a) obtaining a DNA sample from a patient in need of detection of a cancer; (b) incubating said DNA sample with a target-site probe wherein said target site probe and a target polynucleotide comprising a CpG site form a bubble complex wherein said bubble complex comprises a first double-stranded region, a single-stranded non-complementary region, and a second double-stranded region; (c) deaminating the target polynucleotide under conditions which convert unmethylated cytosine residues while leaving the methylated cytosine residues unaltered; (d) incubating said deaminated target polynucleotide with an initiator, a terminator, and an RNA-polymerase; (e) synthesizing an oligonucleotide transcript that is complementary to said CpG site from said target polynucleotide, wherein said initiator is extended until said terminator is incorporated into said oligonucleotide transcript thereby synthesizing multiple reiterative oligonucleotide transcripts; (f) detecting or quantifying said reiteratively synthesized oligonucleotide transcripts thereby detecting methylated cytosine residues; and comparing the results with those obtained similarly from a control sample, thereby detecting a likelihood of the cancer.