Patent ID: 9056340
Filing Date: 2015-06-16
Classification: B09C,C02F,C12N

Abstract:
1. A method of decreasing a concentration of a hydrocarbon contaminant contained within a contaminated region through in situ anaerobic oxidative bioremediation of the hydrocarbon contaminant by native microorganisms that are present within the contaminated region, the method comprising: supplying a first bioremediation formulation that includes a cationic ion exchange resin to the contaminated region to promote metabolism of the hydrocarbon contaminant by the native microorganisms via anaerobic microbial respiration; and subsequent to the supplying the first bioremediation formulation, supplying a second bioremediation formulation to the contaminated region to enhance growth of the native microorganisms and to promote metabolism of the hydrocarbon contaminant by the native microorganisms via anaerobic microbial respiration, wherein the second bioremediation formulation includes: (i) a sulfate salt that comprises 20-40 wt % of the second bioremediation formulation; (ii) a nitrate salt that comprises 1-50 wt % of the second bioremediation formulation; (iii) a phosphate salt that comprises 15-25 wt % of the second bioremediation formulation; (iv) a complex sugar that comprises 3-7 wt % of the second bioremediation formulation; and (v) Brewer's yeast that comprises 5-10 wt % of the second bioremediation formulation.