Patent ID: 6210663
Filing Date: 2001-04-03
Classification: A61K,C12N

Abstract:
A method of enhancing mammalian mucosal immunity to the repeated presentation of a viral antigen from a virus which can infect said mammal via the mucosal tissue, said method comprising the steps of:(a) administering via an intramuscular or intradermal route of administration an effective amount of a priming DNA composition which comprises a plasmid comprising a DNA sequence encoding said viral antigen under the control of regulatory sequences directing expression thereof in a mammalian cell; and(b) subsequently administering intranasally to said mammal an effective amount of a boosting composition which comprises a recombinant adenovirus comprising a nucleotide sequence encoding said antigen under the control of a regulatory sequence directing expression thereof in a mammalian cell,wherein the immune responses induced to said antigen are enhanced in serum and at a mucosal site, and wherein mammalian immune responses to the adenovirus antigens of said recombinant adenovirus are reduced.