Patent ID: 7157222
Filing Date: 2007-01-02
Classification: A01N,A61K

Abstract:
1. A method for removing a tissue or organ from vitrification in a solution containing cryoprotectant, comprising: warming the vitrified tissue or organ in said solution containing cryoprotectant at a rate less than 50° C. per minute to a temperature between −80° C. and the glass transition temperature; further warming the tissue or organ in said solution at a rate greater than 80° C. per minute to a temperature above −75° C., wherein, at said temperature, the solution is sufficiently fluid that the tissue or organ can be removed therefrom; and immersing the tissue or organ in a series of solutions having decreasing concentrations of cryoprotectant to obtain the tissue or organ in a cryoprotectant-free solution, wherein the method occurs under normal physiological pressure.