Patent ID: 8195405
Filing Date: 2012-06-05
Classification: G01N

Abstract:
1. A method for determining values of equilibrium association constants, stoichiometries of association, incompetent fractions, second and third virial coefficients, and/or molar masses corresponding to self-association of reversibly associating macromolecules in solution at high concentration in a solvent, comprising the steps of: 1. introducing a series of k aliquots of solutions of the molecule of interest at concentrations c 2. determining the concentration c 3. forming the excess Rayleigh ratios R(c 4. representing said excess Rayleigh ratios to correct for thermodynamic non-ideality in terms of said k dilutions c wherein M is the molar mass of the monomer, i the order of each self-associating i-mer, c λ is the wavelength of said illuminating light beam, n the refractive index of said solution, N 5. fitting said representations of said excess Rayleigh ratios to the equations of an association model; and 6. determining from said fitted representations the values of equilibrium association constants, stoichiometries of association, incompetent fractions, second and third virial coefficients, and/or molar masses.