Patent ID: 8207293
Filing Date: 2012-06-26
Classification: A61P,C07K

Abstract:
1. A method of intracellular delivery of a substance of interest, comprising the step of using a maurocalcine-derived peptide vector for transporting said substance of interest into cells, wherein said maurocalcine-derived peptide vector consists of the following amino acid sequence (I): Z-X 1 -X 2 -X 3 -X 4 -X 5 -X 6 -X 7 -X 8 -X 9 -X 10 -X 11 -X 12 -X 13 -X 14 -X 15 -X 16 -X 17 -X 18 -X 19 -X 20 -X 21 -X 22 -X 23 -X 24 -X 25 -X 26 -Z′ (I), in which: X X X X X X X X X X wherein one of X 15 , X 16 , X 17 , X 18 , X 19 and X 20 may be an alanine, Z′ represents an arginine or a lysine, Z is either absent or corresponds to the following amino acid sequence (II): Z 1 -Z 2 -Z 3 -Z 4 -Z 5 -Z 6 -Z 7 -Z 8 -Z 9 -Z 10 -Z 11 -Z 12 -Z 13 -Z 14 -Z 15 -Z 16 -Z 17 -Z 18 -Z 19 -Z 20 -Z 21 -Z 22 -Z 23 -Z 24 -Z 25 -Z 26 -Z 27 -Z 28 -Z 29 -Z 30 -Z 31 -Z 32 -Z 33 -Z 34 -Z 35 (II), in which: Z Z Z Z Z Z Z Z Z Z Z Z Z Z Z wherein one of Z 28 and Z 34 is a lysine or an arginine, wherein one of Z 20 , Z 23 , Z 24 , Z 25 , Z 26 , Z 27 Z 28 , Z 32 , Z 33 and Z 35 may be an alanine, wherein Z 1 to Z 18 , Z 22 and Z 31 are absent, and wherein Z 29 , Z 30 , Z 32 , Z 33 , Z 34 and Z 35 are always present, either alone or in combination with one of Z 27 and Z 28 ; Z 21 , Z 23 Z 24 , Z 25 , Z 26 Z 27 and Z 28 ; Z 20 , Z 21 , Z 23 , Z 24 , Z 25 , Z 26 Z 27 and Z 28 ; or Z 19 , Z 20 , Z 21 , Z 23 , Z 24 , Z 25 , Z 26 , Z 27 and Z 28 , and wherein said peptide vector is different from the sequence SEQ ID NO: 1.