Patent ID: 9187766
Filing Date: 2015-11-17
Classification: A23L,A61K,C07K,C12N,C12P,C12Y,Y02A

Abstract:
1. A method of accumulating a polymeric or high molecular weight molecular product within a bacterial microcompartment in bacterial cytoplasm, said method comprising: a) providing a recombinant bacteria which is genetically transformed to express a heterologous bacterial microcompartment operon and an enzyme fused to a bacterial microcompartment localisation signal, wherein the enzyme is capable of converting a low molecular weight substrate into a polymeric or high molecular weight product, and wherein microcompartment localization signal peptide is capable of targeting the enzyme to the microcompartment, b) incubating the recombinant bacteria with the low-molecular weight substrate, or a precursor of the low molecular weight substrate which is capable of being metabolized to the substrate within the recombinant bacteria, wherein the substrate or precursor is taken up by the bacteria, wherein the substrate enters the microcompartment and the enzyme within the microcompartment converts the substrate to a polymeric or high molecular weight molecular product, and wherein the polymeric or high molecular weight molecular product is accumulated within the microcompartment.