Patent ID: 7709233
Filing Date: 2010-05-04
Classification: C12Q,Y10T

Abstract:
1. A method for detecting heterogeneous nuclear ribonucleoprotein A2/B1 RNA in blood plasma from a human, the method comprising the steps of: a) centrifuging blood from a human and obtaining blood plasma; b) extracting extracellular total RNA from said blood plasma from said human and obtaining the extracellular total RNA; c) amplifying a fraction of the extracellular total RNA or cDNA prepared therefrom, either qualitatively or quantitatively, using primers specific for heterogeneous nuclear ribonucleoprotein A2/B1 RNA, or cDNA therefrom, and producing an amplified product; and d) assaying either quantitatively or qualitatively the amplified product to detect heterogeneous ribonucleoprotein A2/B1 RNA in the blood plasma.