Patent ID: 8895299
Filing Date: 2014-11-25
Classification: A61K,A61Q,C12N

Abstract:
1. A method for the expansion or growth of stem cells without substantially inducing differentiation, the method comprising: collecting cord blood from a human umbilical cord; isolating CD34+ cells from the cord blood; producing live placenta conditioned medium (LPCM) by incubating whole human placenta in a Dulbecco's modified Eagle medium (DMEM) in a sterile container, wherein the whole placenta is submerged; isolating the LPCM; and contacting at least one CD34+ cell with a supplemented DMEM comprising the LPCM and a cytokine cocktail comprising 20 ng/ml IL-3, 250 ng/ml IL-6, 10 ng/ml stem cell factor, 250 ng/ml thrombopoietin, and 100 ng/ml Flt-3L, wherein the concentration of the LPCM in the supplemented DMEM is 50%.