Patent ID: 7101986
Filing Date: 2006-09-05
Classification: C07B,C07H,C40B,Y02P

Abstract:
1. A method of synthesizing an oligonucleotide on a solid support comprising: (a) coupling a nucleoside monomer having a protected hydroxyl group to a free hydroxyl group on a support-bound nucleoside monomer, wherein the hydroxyl group on the coupled nucleoside monomer is protected with a carbonate protecting group and the coupling reaction gives rise to a phosphite triester bond between the support-bound nucleoside monomer and the coupled nucleoside monomer; and (b) contacting the coupled nucleoside monomer with an alpha-effect nucleophile to simultaneously (i) irreversibly remove the carbonate protecting group, and (ii) oxidize the phosphite triester linkage to a phosphotriester linkage.