Patent ID: 8067357
Filing Date: 2011-11-29
Classification: C07K

Abstract:
1. An annexin conjugate comprising a variant of annexin A5 covalently bound to a recognizable compound; wherein the annexin A5 comprises the amino acid sequence of SEQ ID NO:1 and one or more polar amino acids selected from Glu, Gln, Asp, Asn, Arg and Lys, in helices IA, ID, IIA, IID, IIIC, IIID and IVE and in stretches connecting helices IC and ID, IIE and IIIA, IIIC and IIID, IIID and IIIE, and IVA and IVB, of SEQ ID NO: 1 is replaced by a non-polar amino acid to impair internalisation of the variant of annexin A5 into a cell, wherein the recognizable compound is bound to a cysteine residue of the variant of annexin A5, and wherein the variant of annexin A5 binds to at least one phospholipid.