Patent ID: 9198976
Filing Date: 2015-12-01
Classification: A61K,A61P,Y02A

Abstract:
1. A method for preparing a conjugate vaccine, the method comprising: reacting a polysaccharide with an oxidizing agent, whereby a solution of an aldehyde-activated polysaccharide is obtained; buffer exchanging the solution of the aldehyde-activated polysaccharide to a pH of from 7 to 8; reacting a protein with carbohydrazide or a dihydrazide in the presence of 1-[3-(dimethylamino)propyl]-3-ethyl carbodiimide hydrochloride at a pH of from 6 to 7, whereby a solution of an hydrazide-activated protein is obtained; raising a pH of the solution of the hydrazide-activated protein to from 7.0 to 11; buffer exchanging the solution of the hydrazide-activated protein to a pH of from 10.0 to 11.0; reacting the aldehyde-activated polysaccharide with the hydrazide-activated protein at a pH of from 6 to 8, whereby a conjugate comprising one or more C═N double bonds is obtained; and reducing the C═N double bonds of the conjugate to C—N single bonds, whereby a conjugate vaccine capable of stimulating an immune response is obtained.