Patent ID: 7837848
Filing Date: 2010-11-23
Classification: G01N

Abstract:
1. A method for separating the constituents of a mixture M comprising electrically charged compounds, implementing a capillary electrophoresis in a single capillary, wherein said method comprises: (A) introducing and migrating the compounds of the mixture M in said capillary according by the capillary electrophoresis, so as to produce a separation of the compounds of the mixture M in the capillary according to their migration speed under the conditions of step (A); (B) isolating in the capillary a fraction F of the compounds separated in step (A), the isolating consisting of at least one of the following steps: (C) under an effect of a potential difference applied between the inlet and the outlet of the capillary, introducing, into the capillary containing the isolated fraction F, a separating medium MS having a migration speed greater than that of the compounds of said fraction F, such that said separating medium MS catches up with said fraction F in the capillary, and modifies the electrophoresis conditions for this fraction, and maintaining the potential difference until there is separation of the compounds included in the fraction F as a function of their migration speed under the new electrophoresis conditions obtained.