Patent ID: 7700326
Filing Date: 2010-04-20
Classification: C12Q

Abstract:
1. A method for distinguishing wild-type classical swine fever virus (CSFV) from lapinized CSF vaccine viruses in an infected subject comprising: a) extracting total RNA from said subject; b) providing a pair of primers for polymerase chain reaction (PCR) comprising a forward degenerate primer selected from SEQ ID NO:3 and a reverse degenerate primer selected from SEQ ID NO:4, SEQ ID NO:6 or SEQ ID NO:7; c) using said RNA as template and performing a reverse transcription-PCR (RT-PCR) with said pair of primers provided in step b); and d) determining the existence of said wild-type CSFV or said lapinized CSF vaccine viruses according to RT-PCR products which said vaccine viruses is at least 12 nucleotides longer than said wild-type CSFV by electrophoresis.