Patent ID: 9029122
Filing Date: 2015-05-12
Classification: A21D,A23K,A61P,C12N,C12P

Abstract:
1. A recombinant oleaginous microbial host cell for improved production of at least one long-chain polyunsaturated fatty acid, said host cell comprising at least one isolated polynucleotide encoding a polypeptide having at least acyl-CoA:lysophospholipid acyltransferase activity, wherein the polypeptide comprises (i) at least 90% amino acid identity, based on the Clustal W method of alignment, compared to the amino acid sequence of SEQ ID NO:11, and (ii) at least one membrane bound O-acyltransferase protein family motif selected from the group consisting of: SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:28; wherein the isolated polynucleotide is operably linked to at least one regulatory sequence, and further wherein the host cell has at least one improvement selected from the group consisting of: (i) an increase in C (ii) an increase in delta-4 desaturation conversion efficiency compared to a control host cell.