Patent ID: 6063284
Filing Date: 2000-05-16
Classification: B01D,G01N

Abstract:
A preparative chromatographic closed loop steady state cyclical process utilizing a single column comprising, at steady state conducting the following steps:a. establishing and maintaining a circulating chromatographic profile through said column and through one solvent pump;b. discontinuously and at each cycle injecting a sample comprising at least two components, into the interior of said maintained circulating profile of said single column, said injecting being conducted by an injection loop controlled in an inject position by an injection valve so as to inject the sample in the loop into the interior of said circulating profile, said injection valve remaining in the inject position from the time of injection until all of the profile has eluted from the column, and then adjusting the injection valve to a load position to load the injection loop when all the profile is on the column, andc. collecting, discontinuously and periodically, at least two enriched fractions from said circulating profile.