Patent ID: 6413740
Filing Date: 2002-07-02
Classification: A61K,A61P,C07K

Abstract:
An isolated nucleic acid molecule comprising a nucleotide sequence encoding the MuSK-activating C-terminal fragment (portion) of human agrin, wherein the nucleotide sequence is selected from:(a) nucleotide sequence comprising the coding region of the MuSK-activating C-terminal fragment (portion) of human agrin contained in the vector designated as pBL-hAgrin 1 American Type Culture Collection Accession No. 97378); (b) a nucleotide sequence whose complement hybridizes under stringent conditions of: 0.15 NaCl/0.015 sodium citrate/0.1% bovine serum albumin/0.1% Ficoll/0.1% polyvinylpyrrolidone/50 mM sodium phosphate buffer at pH 6.5 with 750 mM NaCl, 75 mM sodium citrate at 42Â° C. and a final wash step of 65Â° C. to the nucleotide sequence of (a) and which encodes the MuSK-activating C-terminal fragment (portion) of human agrin; or (c) a nucleotide sequence that, as a result of the degeneracy of the genetic code, differs from the nucleotide sequence of (a) or (b) and which encodes the MuSK-activating C-terminal fragment (portion) of human agrin.