Patent ID: 9186418
Filing Date: 2015-11-17
Classification: A61K,A61P

Abstract:
1. A method of identifying one or more tumor associated antigens of a target tumor comprising the steps of: (a) transfecting genomic or cDNA from cells of a target tumor into recipient cells expressing at least one MHC determinant that is allogeneic to a plurality of recipient animals; (b) diluting a suspension of the transfected recipient cells into a plurality of cell pools; (c) expanding the cells of the plurality of cell pools of step (b); (d) immunizing each of the recipient animals with a portion of each of the expanded cell pools of step (c); (e) isolating spleen cells from the recipient animals immunized according to step (d); (f) incubating the isolated spleen cells with the target tumor cells; (g) detecting an immune response of the spleen cells to the target tumor cells and identifying the cell pool that generates the highest level of immune response (the immuno (h) optionally enriching the immuno (i) isolating RNA from the cells of the immuno (j) performing microarray analysis on RNA isolated from the cells of the immuno (k) identifying one or more genes expressed at increased levels in the cells from the immuno wherein the one or more genes identified are tumor associated antigens of the target tumor.