Patent ID: 8557524
Filing Date: 2013-10-15
Classification: C12Q

Abstract:
1. A method of detecting methiceillin-resistant S. aureus (MRSA) in a sample, wherein said sample contains one or a mixture of bacterial species, said method comprising: (a) amplifying a mecA polynucleotide sequence to produce an amplified mecA polynucleotide sequence; (b) determining a cycle threshold (CT) value of said amplified mecA polynucleotide sequence; (c) amplifying a bridging region polynucleotide sequence from said sample to produce an amplified bridging region polynucleotide sequence; (d) determining a CT value of said amplified bridging region polynucleotide sequence; (e) amplifying an (f) determining a CT value of said amplified (g) comparing said CT values of said amplified mecA, amplified bridging region, and amplified wherein if said CT values of each of said amplified mecA polynueleotide, said amplified bridging region polynueleotide, and said amplified