Patent ID: 6312929
Filing Date: 2001-11-06
Classification: A61K,C07K,C12Q,Y10S

Abstract:
A method of performing an amplification reaction, comprising:(a) combining in an aqueous solution:(i) a target probe, a first control probe and a second control probe;(ii) a first 5' primer, a first 3' primer and a target template, the target template comprising a hybridization site for the first 5' primer, the first 3' primer and the target probe;(iii) a first control template, the first control template comprising a hybridization site for the first 5' primer, the first 3' primer and the first control probe; and(iv) a second 5' primer, a second 3' primer and a second control template, the second control template comprising a hybridization site for the second 5' primer, the second 3' primer, the target probe and a second control probe;(b) performing an amplification reaction to create amplification products; and(c) quantifying binding of the target probe, first control probe and second control probe to the amplification products.