Patent ID: 7582430
Filing Date: 2009-09-01
Classification: A61K,C12Q

Abstract:
1. A method for detecting an analyte with an immunoliposome-nucleic acid amplification assay, comprising: encapsulating 50 to 1,000 identical nucleic acid segments within closed shell liposomal bilayers, associating receptors to the extravesicular surface of said liposomal bilayers, exposing the receptors to an immobilized target analyte, which binds to the liposomal bilayer associated receptors; removing unbound liposomal bilayers; lysing the bound liposomal bilayers to release the nucleic acid segments; amplifying the nucleic acid segments released from said liposomal bilayers, and detecting the amplification products of the nucleic acid segments to quantify the amount of the target analyte.