Patent ID: 6033890
Filing Date: 2000-03-07
Classification: C12N,C12Q,G01N

Abstract:
A mutant glucose-6-phosphate dehydrogenase (G6PDH) that is the expression product of a mutated DNA sequence encoding a G6PDH, the mutant G6PDH being derived from a precursor G6PDH from a Leuconostoc organism by the deletion of, or substitution for, at least two lysines per subunit, or the introduction of at least one cysteine per subunit, by insertion of said cysteine or substitution of said cysteine for an amino acid residue other than cysteine in said precursor G6PDH wherein said mutant G6PDH has the ability to catalyze the oxidation of D-glucose-6-phosphate to D-glucono-.delta.-lactone-6-phosphate by utilizing either NAD.sup.+ or NADP.sup.+.