Patent ID: 6031091
Filing Date: 2000-02-29
Classification: C07F,C07H

Abstract:
A linking reagent having the formula: ##STR3## wherein: D is a protecting group which may be removed to permit said linking reagent to be coupled to a second nucleotide;L.sup.1 is a first linker-arm having the formula:(CH.sub.2).sub.k --NH--(CO--(CH.sub.2).sub.q --NH).sub.r --Mwherein M is a protecting group, or a label stable to DNA synthesis and deprotection conditions,k is an integer between 1 and 4, inclusive,q is an integer between 1 and 11, inclusive,r is either 0, 1, or 2, provided that k+1+(2+q)r is an integer between 1 and 25 inclusive;L.sup.2 is H or L.sup.1 ; andZ is H, a cleavable ester attached to a DNA synthesis support, or a reactive phosphorus-containing group able to couple to or activatable for coupling to an OH group of a first nucleotide.