Patent ID: 6489155
Filing Date: 2002-12-03
Classification: C12N,C12Y

Abstract:
An isolated gene having a DNA sequence encoding a polypeptide capable of degrading a sulfated-fucose-containing polysaccharide or having a functionally identical activity, wherein said gene is selected from the following:(1) a gene having a DNA sequence encoding a polypeptide containing an amino acid sequence represented by either SEQ ID NO: 1 or SEQ ID NO: 2; wherein said polypeptide has a degrading activity which degrades a sulfated-fucose-containing polysaccharide having the following physiochemical properties: (a) constituting saccharide substantially free from uronic acid; and (b) substantially incapable of being degraded by the fucoidanase produced by Flavobacterium sp. SA-0082 (FERM BP-5402); (2) a gene having a DNA sequence encoding a polypeptide containing an amino acid sequence represented by either SEQ ID NO: 3 or SEQ ID NO: 4; wherein said polypeptide had a degrading activity which degrades a sulfated-fucose-containing polysaccharide having the following physiochemical properties: (a) constituting saccharide containing uronic acid; and (b) being degraded by the fucoidanase produced by Flavobacterium sp SA-0082 (FERM BP-5402) and degrades the said sulfated-fucose-containing polysaccharide whereby at least one of the following formulae is liberated; and(3) a gene having a DNA sequence which hybridizes to (1) or (2) in 6Ã—SSC, 1% SDS, 5Ã—Denhardt's solution and 100 &mgr;g/ml of salmon sperm DNA at 65Â° C.