Patent ID: 6573092
Filing Date: 2003-06-03
Classification: C12N

Abstract:
A method of producing a eukaryotic viral vector, which method comprises:contacting a eukaryotic cell, which comprises a genorne and which does not comprise a unique enzyme that nicks or cleaves a DNA molecule, simultaneously or sequentially, in either order, with (i) a unique enzyme that nicks or cleaves a DNA molecule and (ii) a recombinant phage vector, wherein the recombinant phage vector comprises the DNA molecule comprising (a) a eukaryotic viral vector genome comprising a coding sequence, (b) a phage packaging site and a unique enzyme site that are not contained within the eukaryotic viral vector genome, and (c) a promoter that is operably linked to the coding sequence, whereupon the DNA molecule enters the eukaryotic cell and the unique enzyme nicks or cleaves the unique enzyme site in the DNA molecule in the eukaryotic cell, whereupon a eukaryotic viral vector is produced.