Patent ID: 7657379
Filing Date: 2010-02-02
Classification: C12Q,C40B,G01N,G16B

Abstract:
1. A method for determining a biological function of a cellular constituent that is perturbed in one or more landmark response profiles that are most similar to a first response profile, comprising: (a) receiving a first response profile comprising measured amounts of a plurality of different cellular constituents in a first cell of a cell type or type of organism, said first cell having a first perturbation to said first cellular constituent; (b) comparing said first response profile to a plurality of landmark response profiles stored in a database to determine a measure of similarity between said first response profile and each said landmark response profile in said plurality of landmark response profiles, each said landmark response profile comprising measured amounts of said plurality of different cellular constituents in a second cell of said cell type or type of organism, said second cell having a second perturbation to a second cellular constituent associated with a known biological function, wherein said plurality of landmark response profiles comprises landmark response profiles corresponding to respective perturbations to at least 118 different genes of a cell of said cell type or type of organism, and wherein said measured amounts in said first response profile and in said plurality of landmark response profiles are all measured amounts of transcripts or are all measured amounts of proteins; (c) determining one or more landmark response profiles most similar to said first response profile based on the measures of similarity determined in step (b); and (d) identifying the known biological function associated with the one or more second cellular constituents that are perturbed in the one or more second cells corresponding to the respective one or more landmark response profiles determined to be most similar to said first response profile in step (c); wherein steps (a), (b), (c), and (d) are implemented on a suitably programmed computer.