Patent ID: 8124329
Filing Date: 2012-02-28
Classification: A01N

Abstract:
1. A method for hypothermic preservation of biological tissue for later recovery to a viable state, comprising the steps of: (a) in a closed environment, flushing a biological tissue with a clathrate inducing gas mixture consisting of a clathrate inducing gas and oxygen, wherein the clathrate inducing gas is selected from the group consisting of: sulfur hexafluoride and xenon, wherein the clathrate inducing gas is in a concentration in the clathrate inducing gas mixture within a range of about 75 mole percent to 95 mole percent, wherein said flushing displaces gases surrounding the biological tissue with the clathrate inducing gas mixture; (b) pressurizing the flushed biological tissue and the resultant surrounding clathrate inducing gas mixture of step (a) to a pressure of 100 psig to form clathrates inside the biological tissue of step (a) at a first temperature in a range of 1° C. to 5° C.; and (c) cooling the biological tissue and the surrounding clathrate inducing gas mixture of step (b) to a second temperature of −20° C.