Patent ID: 9200252
Filing Date: 2015-12-01
Classification: A61K,C12N

Abstract:
1. A method for producing retinal cells comprising: a) culturing the human ESCs or IPSCs as floating aggregates in a medium comprising IGF-1 DKK-1, and NOGGIN to differentiate the human embryonic stem cells into eye field precursor cells; b) culturing the eye field precursor cell floating aggregates in a medium comprising IGF-1 DKK-1, NOGGIN, and FGF-2 to differentiate the eye field precursor cells into retinal progenitor cells; c) inducing differentiation of the retinal progenitor cells to retinal cells by culturing the retinal progenitor cells in a medium comprising a differentiation factor selected from the group consisting of: a Wnt signaling pathway activator; wherein the medium does not comprise exogenous retinoic acid and exogenous sonic hedgehog.