Patent ID: 7517670
Filing Date: 2009-04-14
Classification: A61P,C07K,C12P,Y10S

Abstract:
1. A method for producing a recombinant antibody having increased Fc mediated cellular cytotoxicity or increased Fc receptor binding affinity, comprising: (a) providing a mammalian host cell that expresses a recombinant antibody comprising an IgG Fc region containing N-linked oligosaccharides; (b) glycoengineering said host cell, wherein said glycoengineering comprises genetically manipulating said host cell so that said host cell has a decreased level of activity of at least one glycoprotein-modifying glycosyltransferase; (c) culturing said glycoengineered host cell under conditions which permit the production of said recombinant antibody; and (d) isolating said recombinant antibody; wherein said recombinant antibody has increased Fc-mediated cellular cytotoxicity or increased Fc receptor binding affinity compared to the corresponding antibody produced by the same host cell that has not been glycoengineered.