Patent ID: 6037124
Filing Date: 2000-03-14
Classification: C12Q

Abstract:
A method of analyzing oligonucleotide targets comprising the steps; of:a) providing a solid support fabricated of carboxylated polyvinylidene fluoride, wherein the surface of the carboxylated polyvinylidene fluoride has carboxyl groups;b) immobilizing a binding protein by non-covalent interaction of the binding protein with the surface;c) attaching oligonucleotide probes to the support, said probes having a specific binding partner to said binding protein, whereby the oligonucleotide probes are bound to the surface of the support via a binding protein-specific binding partner interaction between the specific binding partner and the binding protein;d) applying oligonucleotide targets to the surface under hybridizing conditions; ande) determining if hybridization occurs thereby detecting the presence or absence of oligonucleotide targets having sequences complementary to the oligonucleotide probes.