Patent ID: 7341860
Filing Date: 2008-03-11
Classification: A61K,A61P,C12N

Abstract:
1. A microorganism comprising a regulated antigen delivery system (RADS), wherein the RADS comprises: (a) a vector comprising (1) a gene encoding a desired gene product operably linked to a second control sequence; (2) a first origin of replication (ori) conferring vector replication using DNA Polymerase III; and (3) a second ori conferring vector replication using DNA Polymerase I, wherein the second ori is operably linked to a first control sequence repressible by a first repressor, and wherein the runaway vector does not comprise a phage lysis gene; and (b) a gene encoding a first repressor operably linked to a first activatible control sequence, wherein said microorganism additionally comprises a balanced lethal host vector system comprising a lack of a functioning essential gene, wherein the essential gene is a gene necessary for synthesis of essential cell wall constituent diaminopimelic acid (DAP), on the chromosome and the presence of a recombinant functioning copy of the essential gene on the vector.