Patent ID: 6441269
Filing Date: 2002-08-27
Classification: C12N,C12Q

Abstract:
A method for obtaining isolated cells of a subpopulation of cells defining a cell type defined by the expression of an endogenous gene, the method composing the steps of;isolating a population of cells from a transgenic animal comprising a transgene, wherein the transgene is inserted into a preselected, endogenous target gene comprising an endogenous promoter, expression of the transgene is controlled by the endogenous promoter and in a manner such that the expression of the transgene matches the expression of the target gene without decreasing native target gene expression, and the transgene encodes a selectable marker; detecting in the population of cells a subpopulation of cells expressing the selectable marker and defining a cell type; and isolating cells of the subpopulation, wherein the subpopulation is of neurons and the animal is a mouse.