Patent ID: 8124357
Filing Date: 2012-02-28
Classification: G01N

Abstract:
1. A cell comprising: a) a membrane-associated Clostridial toxin substrate, said substrate comprising b) a membrane-associated second member of a FRET pair; and c) a receptor that binds a Clostridial toxin; wherein the membrane targeting domain comprises a SNAP-25 or Syntaxin peptide which directs a Clostridial toxin substrate to the cell membrane; wherein the Clostridial toxin recognition sequence intervenes between the first FRET pair member and the membrane targeting domain; wherein the cell is capable of Clostridial toxin intoxication; wherein the FRET pair comprises an acceptor having an absorbance spectrum overlapping the emission spectrum of a donor fluorophore; and wherein under the appropriate conditions, resonance energy transfer is exhibited between the acceptor and the donor fluorophore.