Patent ID: 6846649
Filing Date: 2005-01-25
Classification: A61K,A61P,C07K

Abstract:
1. A process of producing a purified recombinant human mannan binding lectin (MBL) composition which is free of monomers of the 96 kDa MBL subunit which comprises providing a starting recombinant human MBL composition comprising both (a) monomers of the 96 kDa MBL subunit and (b) at least one oligomer selected from the group consisting of dimers, trimers, tetramers, pentamers and hexamers of said 96 kDa MBL subunit, wherein said 96 kDa subunit comprises the polypeptide of SEQ ID NO:1 separating (b) from (a) by subjecting the composition to affinity chromatography using a carbohydrate-derivatized matrix having affinity for at least one oligomer of (b) and which substantially lacks affinity for said monomers of (a), and thereby obtaining a purified composition which comprises at least one oligomer of (b) and which is without any detectable amount of said monomer (a) of said 96 kDa MBL subunit.