Patent ID: 9005961
Filing Date: 2015-04-14
Classification: A61K,A61P,C07K,C12N

Abstract:
1. An expression vector for producing an infectious recombinant Schwarz strain of measles virus comprising: A) the nucleotide sequence encoding the full length antigenomic (+)RNA strand of the measles virus Schwarz strain (from position 83 to position 15976 of SEQ ID NO: 82); B) a T7 promoter sequence comprising a GGG motif at its 3′ end, operably linked to the nucleotide sequence of A; C) a hammerhead ribozyme sequence (from position 29 to position 82 of SEQ ID NO: 82) located adjacent to the GGG motif at one end and adjacent to the first nucleotide of the nucleotide sequence encoding the full length anti-genomic (+)RNA strand of the measles virus Schwarz strain at the other end; D) a T7 terminator sequence operably linked to the nucleotide sequence of A; E) the sequence of a hepatitis delta virus ribozyme located adjacent to the last nucleotide of the nucleotide sequence encoding the full length anti-genomic (+)RNA strand of the measles virus Schwarz strain; and F) a heterologous coding sequence encoding a heterologous amino acid sequence.