Patent ID: 6329571
Filing Date: 2001-12-11
Classification: A01H,C12N

Abstract:
A method for transforming rice comprising transforming immature embryo cells of Indica rice by Agrobacterium tumefaciens and selecting transformed cells by using a drug for selection, characterized in that a medium containing 2000 to 4000 mg/l of KNO.sub.3, 60 to 200 mg/l of MgSO.sub.4, 200 to 600 mg/l of KH.sub.2 PO.sub.4, 100 to 450 mg/l of CaCl.sub.2, 200 to 600 mg/l of (NH.sub.4).sub.2.SO.sub.4, 1 to 7 mg/l of H.sub.3 BO.sub.3, 2 to 20 mg/l of MnSO.sub.4, 20 to 50 mg/l of EDTA or a salt thereof, 3 to 8 mg/l of Fe, 50 to 200 mg/l of myoinositol, 0.5 to 10 mg/l of 2,4-dichlorophenoxyacetic acid, 0.01 to 5 mg/l of a cytokinin, 5000 to 80,000 mg/l of a sugar, and a gelling agent, which medium has a pH of 4.5 to 6.5, is used as a medium for selecting said transformed cells.