Patent ID: 8129355
Filing Date: 2012-03-06
Classification: A61P,C07C,C07D,C07F,C07K,C12Q

Abstract:
1. A method of modulating the expression of a gene in a host cell comprising the steps of: a) introducing into the host cell a gene expression modulation system comprising: i) a first gene expression cassette that is capable of being expressed in a host cell comprising a polynucleotide sequence that encodes a first hybrid polypeptide comprising: ii) a second gene expression cassette that is capable of being expressed in the host cell comprising a polynucleotide sequence that encodes a second hybrid polypeptide comprising: iii) a third gene expression cassette that is capable of being expressed in a host cell, wherein the gene expression cassette comprises a polynucleotide sequence comprising: b) introducing into the host cell a ligand of the formula: wherein Q n=1 or 2; R wherein in said substituted phenyl, 1-naphthyl, 2-naphthyl, phenyl(C the heterocyclic ring contains from one to three heteroatoms selected from N, O, or S; and one to four substituents are independently selected from the group consisting of cyano, nitro, halo, aminocarbonyl, aminothiocarbonyl, carboxy, formyl, hydroxy, amino, carbamoyl, (C R wherein R R wherein in said substituted phenyl, 1-naphthyl, 2-naphthyl, phenyl(C R wherein in said substituted phenyl, 1-naphthyl, 2-naphthyl, phenyl(C R wherein R whereby upon introduction of the ligand into the host cell, expression of the gene of iii)c) is modulated.