Patent ID: 6448051
Filing Date: 2002-09-10
Classification: C12P,Y10S

Abstract:
Process for the preparation of 4-(R)-hydroxycyclopent-2-en-1(S)-acetate of the formula 2which comprises reacting meso-cyclopent-2-en-1,4-diacetate of formula 1with a whole cell enzyme selected from the group consisting of Trichosporon beigelii, Trichosporon beigeii, Trichosporon sp., Trichosporon sp. and Trichosporon capitatum in a mixture of a buffer and an organic solvent, the buffer to organic solvent ratio being in the range of from 4:1 to 24:1, stirring the reaction mixture at a temperature ranging between 25-30Â° C. for a period ranging from 10-16 hrs, filtering the reaction mixture to remove the enzyme and extracting the resultant compound with all organic solvent, removing the solvent to obtain the desired product.