Patent ID: 8697144
Filing Date: 2014-04-15
Classification: A23J,A23V

Abstract:
1. A method of producing a canola protein isolate having a canola protein content of at least 90 wt % (N×6.25) d.b., which consists essentially of: (a) extracting canola oil seed meal at a temperature of at least about 5° C. to cause solubilization of canola protein from the meal and to form an aqueous protein solution having a protein content of about 5 to about 40 g/L and a pH of about 5 to about 6.8 and spent oil seed meal, (b) separating the aqueous protein solution from the spent oil seed meal, (c) increasing the protein concentration of the aqueous protein solution to about 50 to about 250 g/L while maintaining the ionic strength substantially constant by using a selective membrane technique to provide a first concentrated protein solution, (d) optionally diafiltering the first concentrated protein solution, (e) adding calcium salt solution to the first concentrated and optionally diafiltered protein solution to a conductivity of about 15 to about 25 mS to cause a precipitate to form in the first concentrated protein solution, (f) removing the precipitate from the first concentrated protein solution, (g) diluting the clarified first concentrated protein solution with about 2 to about 20 volumes of water having a temperature of about 2° to about 90° C., (h) acidifying the resulting solution to a pH of about 2.5 to about 4.0 to produce an acidified clear protein solution, (i) increasing the concentration of the acidified clear protein solution to about 50 to about 200 g/L while maintaining the ionic strength substantially constant by using a selective membrane technique to provide a second concentrated protein solution, (j) optionally diafiltering the second concentrated protein solution, and (k) drying the second concentrated protein solution to provide a canola protein isolate having a protein content of at least 90 wt % (N×6.25) d.b.