Patent ID: 7794989
Filing Date: 2010-09-14
Classification: C07K,C12P

Abstract:
1. A coryneform bacterium having L-glutamic acid-producing ability, wherein said coryneform bacterium is modified so that L-glutamic acid-producing ability of the bacterium is enhanced as compared to a non-modified bacterium, wherein said bacterium is modified in a manner selected from the group consisting of: a) overexpressing a yggB gene, b) enhancing the activity of the yggB protein by: c) combinations thereof, wherein said mutation is selected from the group consisting of: (A) a deletion in said region, (B) insertion of an insertion sequence or transposon into said region, (C) replacement of the proline at position 424 and/or the proline at position 437 in SEQ ID NO: 6, 62, 68, 84, or 85 with another amino acid, and (D) combinations thereof; wherein said yggB gene is selected from the group consisting of: (i) a DNA comprising nucleotides 1437 to 3035 of SEQ ID No: 5, (ii) a DNA that is able to hybridize with a nucleotide sequence complementary to nucleotides 1437 to 3035 of SEQ ID No: 5 under stringent conditions comprising washing with 1×SSC, 0.1% SDS at 60° C., and wherein said DNA is at least 95% identical to nucleotides 1437 to 3035 of SEQ ID NO: 5 and increases L-glutamic acid-producing ability of a coryneform bacterium when it is introduced into the coryneform bacterium, (iii) a DNA comprising nucleotides 507 to 2093 of SEQ ID No: 61, (iv) a DNA that is able to hybridize with a nucleotide sequence complementary to nucleotides 507 to 2093 of SEQ ID No: 61 under stringent conditions comprising washing with 1×SSC, 0.1% SDS at 60° C., and wherein said DNA is at least 95% identical to nucleotides 507 to 2093 of SEQ ID NO: 61 and increases L-glutamic acid-producing ability of a coryneform bacterium when it is introduced into the coryneform bacterium, (v) a DNA comprising nucleotides 403 to 2001 of SEQ ID No: 67, (vi) a DNA that is able to hybridize with a nucleotide sequence complementary to nucleotides 403 to 2001 of SEQ ID No: 67 under stringent conditions comprising washing with 1×SSC, 0.1% SDS at 60° C., and wherein said DNA is at least 95% identical to nucleotides 403 to 2001 of SEQ ID NO: 67 and increases L-glutamic acid-producing ability of a coryneform bacterium when it is introduced into the coryneform bacterium, (vii) a DNA comprising nucleotides 501 to 2099 of SEQ ID No: 83, and (viii) a DNA that is able to hybridize with a nucleotide sequence complementary to nucleotides 501 to 2099 of SEQ ID No: 83 under stringent conditions comprising washing with 1×SSC, 0.1% SDS at 60° C., and wherein said DNA is at least 95% identical to nucleotides 501 to 2099 of SEQ ID NO: 83 and increases L-glutamic acid-producing ability of a coryneform bacterium when it is introduced into the coryneform bacterium.