Patent ID: 9066909
Filing Date: 2015-06-30
Classification: A01N,A61K,C12N

Abstract:
1. A method for restoring a metabolic profile in red blood cells (RBCs), comprising: a) obtaining whole blood from a donor; b) removing leukocytes from the whole blood, to produce a volume of leukoreduced blood; c) mixing the leukoreduced blood with an RBC enhancement composition comprising a rejuvenating material selected from the group consisting of inosine, adenine, pyruvate, phosphate, and mixtures thereof; and d) incubating the mixture of leukoreduced blood and RBC enhancement composition, thereby restoring the metabolic profile in the RBCs and forming treated leukoreduced RBCs; and e) administering the treated leukoreduced RBCs to a subject in need thereof, wherein the levels of one or both of adenosine triphosphate (ATP) and 2,3-diphosphoglycerate (2,3-DPG) in the treated leukoreduced blood after the step of incubating are restored to levels equal to or greater than the levels of ATP and 2,3-DPG found in fresh blood, and wherein the removing, mixing, incubating, and administering are performed within 24 hours of the obtaining of the whole blood from the donor.