Patent ID: 8211654
Filing Date: 2012-07-03
Classification: G01N

Abstract:
1. A method for assaying sugar-mediated transport of glycoconjugates contained in a glycorandomized library that occurs via one or more facilitative glucose transporters (GLUTs), comprising: (a) providing a glycorandomized library comprising glycoconjugates with different attached sugars, wherein the glycoconjugates are sufficiently small to be capable of directly passing through the cell membrane of a cell utilizing one or more GLUTs; (b) contacting each of said glycoconjugates with cells under conditions to allow uptake of the glycoconjugates into the cells; and (c) assessing uptake of said glycoconjugates into the cells, wherein the cells are cancerous, wherein sugar-mediated transport of the glycoconjugates into the cells is quantified relative to a corresponding conjugate lacking the attached sugars.