Patent ID: 8940482
Filing Date: 2015-01-27
Classification: C12Q,G01N

Abstract:
1. A cell based assay for assessing the activity of a Botulinum Neurotoxin (BoNT), comprising: measuring the disappearance of a single fluorescent or luminescent label located at the C-terminus end of a BoNT substrate in a recombinant cell in the presence of a BoNT, wherein the recombinant cell expresses a BoNT substrate, which substrate comprises said single fluorescent or luminescent label at its C-terminus, wherein said BoNT substrate generates a N-terminus fragment and a C-terminus fragment upon cleavage by the BoNT and where the C-terminus fragment and label are degraded by the recombinant cell's ubiquitin proteasome pathway; wherein the substrate is not labeled as a FRET pair.