Patent ID: 7790446
Filing Date: 2010-09-07
Classification: A61K,C07K,C12N

Abstract:
1. An expression system to provide extended episomal replication of hybrid plasmids in eukaryotic cell lines, said system comprising: a) a vector having a mouse polyoma virus core origin obtained from a complete polyoma virus origin by removing enhancer element; a papillomavirus minichromosomal maintenance element (MME), said MME being a Bovine papillomavirus (BPV) MME and containing at least 5 E2-binding sites, said E2-binding sites being SEQ ID NO:9; an optional selection marker; and a gene of interest operably linked within an expression cassette expressing the gene in a eukaryotic cell; and b) a compatible cell line, wherein the vector is expressed, said cell line constitutively expressing BPV-E2 protein and mouse polyomavirus (PyV) LT protein either in presence or in absence of selective pressure.