Patent ID: 8677934
Filing Date: 2014-03-25
Classification: C07K

Abstract:
1. An isolated nucleic acid molecule comprising a polynucleotide sequence encoding an oligopeptide capable of prolonging post-mating depression of receptivity to mating in female insects as compared with wild type DrmSP, wherein said oligopeptide: (i) is derived from the Drosophila melanogaster sex peptide (“DrmSP”) of the amino acid sequence as set forth in SEQ ID NO: 1, said oligopeptide consisting of the N-terminal signal peptide of DrmSP of the amino acid sequence as set forth in SEQ ID NO: 4, the N-terminal motif of DrmSP of the amino acid sequence as set forth in SEQ ID NO: 5 and the C-terminus of DrmSP of the amino acid sequence as set forth in SEQ ID NO: 3, or (ii) is derived from an active fragment of DrmSP comprising the N-terminal signal peptide of DrmSP of the amino acid sequence as set forth in SEQ ID NO: 4, and the C-terminus of DrmSP of the amino acid sequence as set forth in SEQ ID NO: 3, said oligopeptide (i) or active fragment (ii) being made resistant to proteolytic degradation by trypsin at a scissile bond within the C-terminus by neutral or conservative substitution of arginine 25 and/or lysine 26.