Patent ID: 7632633
Filing Date: 2009-12-15
Classification: G01N

Abstract:
1. A method of determining the degree of protein oxidation in a skin sample comprising stratum corneum, the method comprising: (a) providing a skin sample (S) selected from the group comprising: wherein the skin sample (S) comprising said stratum corneum (i) or said oxidized stratum corneum (ii) is contacted and fluorescently labeled with a hydrazine fluorophore, the hydrazine fluorophore selected from the group consisting of fluorescein-thiosemicarbazide; Texas Red hydrazide; biotin hydrazide reacted with fluorescein avidin; dintrophenylhydrazine (DNPH) reacted with anti-DNP antibody and with a fluorescein-labeled secondary antibody specific to the anti-DNP antibody; and DNPH reacted with a fluorescent dye; (b) measuring the fluorescence of the skin sample (S); and then (c) determining the degree of protein oxidation of the skin sample (S),