Patent ID: 8927508
Filing Date: 2015-01-06
Classification: A61K,A61P,C12N

Abstract:
1. A method of generating antibodies in a mammal against recombinant antigens, wherein said antigen is influenza HA, influenza NA, or influenza M2e-NP, using DNA plasmids capable of expressing said antigens in cells of said mammal, comprising: injecting into tissue of said mammal a DNA plasmid consisting essentially of an encoding sequence operably linked to a promoter, wherein the encoding sequence encodes a protein consisting of the antigen, electroporating said tissue with an electroporation device capable of delivering an electrical pulse effective to electroporate cells of said tissue to allow entry of said DNA plasmid and expression of said antigen, and allowing said mammal to respond to said expressed antigen in order to generate antibodies to said antigen wherein the electroporating step comprises delivering a current of 0.5 Amp.