Patent ID: 6500645
Filing Date: 2002-12-31
Classification: C07K,C12N

Abstract:
A DNA construct encoding a precursor polypeptide having the following structure;signal peptide-leader peptide-X1-X2-X3-X4-X5-X6-X7-heterologous protein whereinX1 is Lys or Arg; X2 is Lys or Arg, X1 and X2 together defining a yeast processing site; X3 is Glu or Asp; X4 is Glu or Asp; X5 is a peptide bond or is 1-9 amino acids which may be the same or different; X6 is Pro; and X7 is Lys or Arg, and wherein expression of said DNA construct in yeast results in secretion of a polypeptide having the structure X3-X4-X5-X6-X7-heterologous protein.