Patent ID: 7888057
Filing Date: 2011-02-15
Classification: G01N,Y10S

Abstract:
1. A method of immunologically analyzing plasmin-digested products of stabilized fibrin, the method comprising: (a) bringing a sample suspected of containing plasmin-digested products of stabilized fibrin into contact with a first monoclonal antibody and a second monoclonal antibody, wherein the first monoclonal antibody does not form a sandwich immunocomplex with stabilized fibrin, fibrinogen, or plasmin-digested products of fibrinogen, but reacts with a neoantigen in a D domain of stabilized fibrin which is newly exposed by digesting stabilized fibrin with plasmin, and the second monoclonal antibody does not form a sandwich immunocomplex with stabilized fibrin, fibrinogen, or plasmin-digested products of fibrinogen, but reacts with plasmin-digested products of stabilized fibrin and a neoantigen that is newly exposed in an E domain by digesting stabilized fibrin with plasmin, wherein one of said first or second monoclonal antibodies is carried on a magnetic particle, and the other is labeled with an enzyme label; (b) separating enzyme-labeled monoclonal antibody which forms an immunocomplex via plasmin-digested products of stabilized fibrin with the monoclonal antibody carried on a magnetic particle from enzyme-labeled monoclonal antibody which does not form the immunocomplex; (c) adding a chemiluminescent substrate to the separated immunocomplex to generate a chemiluminescent signal; and (d) analyzing the generated chemiluminescent signal, wherein the amount of chemiluminescent signal correlates with the amount of plasmin-digested products of stabilized fibrin in the sample.