Patent ID: 6303343
Filing Date: 2001-10-16
Classification: B01L,C12Q

Abstract:
A method of performing a cyclic polymerase-mediated reaction, the method comprising:incubating a polymerase, a plurality of template molecules, and a plurality of primer molecules for a first time (t.sub.1), wherein t.sub.1.ltoreq.0.5(t.sub.2), wherein t.sub.2 is the time required for the duplication of at least 90% of the plurality of template molecules when said primer molecules and said polymerase are present in non-rate limiting amounts; andrepeating the incubation of said polymerase, said plurality of template molecules, and said plurality of primer molecules, for time t.sub.1, for a number of cycles, x.sub.1, wherein x.sub.1 is greater than the number of cycles required to produce a detectable amount of product when said polymerase, said plurality of template molecules, and said plurality of primer molecules are incubated for time t.sub.2, wherein the total amount of time required to generate a detectable amount of product using said increased number of said reduced-time cycles is less than the total amount of time required to generate a detectable amount of product sing the standard number of full-time cycles.