Patent ID: 8790870
Filing Date: 2014-07-29
Classification: C12Q

Abstract:
1. A method for predicting quantitative tropism, quantitative tropism being the quantitative shift in HIV-1 co-receptor usage from CCR5 to CXCR4, from CXCR4 to CCR5, or from dual trophic virus to either CCR5 or CXCR4, in a patient infected by HIV comprising: a) using a sample comprising viral genetic material from the patient; b) extraction of viral genetic material from said sample followed by single genome sequencing comprising the following steps: c) prediction of the specific tropism using said genotype sequence with a predictive algorithm comprising the following steps: d) analysis of clonal sequences without predictable phenotype in a single clone biological phenotyping assay, wherein the information obtained after said analysis is loaded into the correlative genotype-phenotype database used in step c), wherein the single clone biological phenotyping assay comprises the following steps: e) prediction of the quantitative tropism based on the information obtained in steps c)1 to c)4 for every single sequence clone present in a sample of a HIV infected patient.