Patent ID: 8367411
Filing Date: 2013-02-05
Classification: A61F,A61L,C12N

Abstract:
1. A method of manufacturing an artificial blood vessel, wherein the method comprises: preparing an image of a target living tissue; extracting a blood vessel formation image comprising only lines representing the blood vessels from the image of the tissue wherein the blood vessel formation image has substantially no contours of the living tissue or noise; adjusting the width of the lines representing the blood vessels in the blood vessel formation image to create an adjusted blood vessel formation image; preparing a photomask which corresponds to the adjusted blood vessel formation image, wherein the solid portions of the photomask replicate the pattern of the adjusted blood vessel formation image; preparing a patterning substrate which comprises a base material and a cell culture layer formed on the base material, wherein the cell culture layer contains a cell adhesive material that has cell adhesive properties and is denatured by action of a photocatalyst when exposed to energy irradiation which in turn enables a formation of a cell adhesion-inhibiting portion; preparing a photocatalyst-containing substrate which comprises a base body and a photocatalyst-containing layer formed on the base body and containing the photocatalyst; arranging the cell culture layer of the patterning substrate and the photocatalyst-containing layer of the photocatalyst-containing substrate to face each other so that the cell culture layer and the photocatalyst-containing layer actually contact each other or face each other at a distance; arranging the photomask which corresponds to the adjusted blood vessel formation image on the base body side of the photocatalyst-containing substrate; irradiating energy through the photomask to the photocatalyst-containing substrate, energy; and adhering a blood vessel cell to a cell adhesive portion in the cell culturing layer; and culturing the blood vessel cell on the cell culturing layer to form an artificial blood vessel.