Patent ID: 6432713
Filing Date: 2002-08-13
Classification: C12M

Abstract:
A method of cultivating a cell or tissue of a living body to be cultivated inside a culture chamber comprising the steps of:forming a sealed space while isolated from the outside into which space gas is supplied, and in which space temperature and humidity conditions are set to maintain an environment necessary for sustaining life; forming a culture circuit unit which is separable from the sealed space; the culture circuit unit comprising a culture fluid bath and the culture chamber which are installed therein and connected to each other by a tube to form a culture circuit through which circuit culture fluid can be circulated from the culture fluid bath to the culture chamber; supplying the culture fluid continuously, intermittently or periodically or by the combination thereof from the culture fluid both so as to circulate the culture fluid in the culture chamber through the culture circuit; rendering the cell or tissue in the culture chamber to be held in a suspending or non-suspending state by holding means in the culture fluid within the culture chamber; and forming a gas absorption portion in the tube of the culture circuit at a front stage of the culture chamber separately from the culture fluid bath, through which the gas in the sealed space permeates and rendering the gas to be absorbed in the culture fluid which flows toward the culture chamber through the tube; wherein when the cell or tissue held by the holding means grows, the holding means is absorbed in the cell or tissue and vanishes.