Patent ID: 6613552
Filing Date: 2003-09-02
Classification: C12N,C12P,C12R,C12Y,Y10S

Abstract:
A method for the production of shikimic acid from a carbon source comprising:a) providing a microbe selected from the group consisting of bacteria, fungi and yeast, wherein the microbe comprises one or more enzyme-encoding recombinant DNA molecules, wherein the encoded enzyme is selected from the group consisting of 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthase, 3-dehydroquinate synthase, 3-dehydroquinate dehydratase, and 3-dehydroshikimate dehydrogenase, and wherein the microbe further comprises an inactivating mutation in at least one DNA molecule which encodes a shikimate kinase isozyme; and b) culturing said microbe in the presence of the carbon source.