Patent ID: 8637238
Filing Date: 2014-01-28
Classification: C12Q,G01N

Abstract:
1. A method of verifying the performance of an optical detection apparatus, the method comprises the following processes (A) through (H): (A) providing an optical detection apparatus having an optical detection performance and temperature control performance that are normal; (B) providing a standard sample containing a nucleic acid sequence; (C) measuring a first optical signal intensity and first melting temperature of the standard sample with the optical detection apparatus having an optical detection performance and temperature control performance that are normal; (D) providing an optical detection apparatus to be verified; (E) measuring a second optical signal intensity of an identical standard sample with the optical detection apparatus to be verified by increasing or decreasing temperature of the identical standard sample with a temperature control unit of the optical detection apparatus to be verified; (F) determining a second melting temperature of the identical standard sample from a change in the optical signal intensity accompanying a change in the temperature; (G) comparing both (i) the second optical signal intensity of the identical standard sample measured in process (E) to the first optical signal intensity of the standard sample determined in process (C) and (ii) the second melting temperature of the identical standard sample determined in process (F) to the first melting temperature of the standard sample determined in process (C); and (H) determining whether the optical detection apparatus to be verified is verified or not based on the comparison in process (G).