Patent ID: 9040309
Filing Date: 2015-05-26
Classification: G01N

Abstract:
1. A binding layer, comprising: a polysaccharide substituted by a plurality of carboxylic groups, the plurality of carboxylic groups comprising a plurality of easily-activated carboxylic groups, wherein at least one β-atom of each of the easily activated carboxylic groups is a carbon atom and remaining β-atoms are independently hydrogen or carbon atoms, the β-carbon atom of each of the easily activated carboxylic groups is linked to a functional group of the polysaccharide through a covalent linkage, the functional group being a native or chemically introduced carboxylic group, or a derivative thereof selected from the group consisting of an ester, an amide, an anhydride, and an acetyl halide, wherein at least a part of the plurality of the easily-activated carboxylic groups are activated carboxylic ester groups capable of binding ligand molecules, the activated carboxylic ester groups being obtained by activation of the carboxylic groups of the easily activated carboxylic groups and being selected from the group consisting of a N-hydroxysuccinimide ester and a N-hydroxysulfosuccinimide ester, and the β-carbon atom of the easily-activated carboxylic groups has no electron withdrawing groups.