Patent ID: 6107272
Filing Date: 2000-08-22
Classification: A61K,C07K,C12N,Y10S

Abstract:
A biologically active polypeptide homodimer wherein each polypeptide of the homodimer comprises a four alpha helical bundle polypeptide and the plasma half-life of the homodimer increased in comparison to wildtype monomer, comprising two polypeptides covalently linked by a least one thioether bond, wherein:a) the first polypeptide comprises a polypeptide with a heterobifunctional cross-linking reagent containing a free sulfhydryl group attached to the polypeptide; andb) the second polypeptide comprises a polypeptide with a heterobifunctional cross-linking reagent containing a maleimido group attached to the polypeptide and at least one thioether bond forms between the free sulfhydryl group of the first polypeptide and the maleimido group of the second polypeptide.