Patent ID: 7393437
Filing Date: 2008-07-01
Classification: A61L,C07K,C12N

Abstract:
1. A method for producing a crosslinked collagen scaffold comprising (a) providing a solution of extracellular collagen monomers at an acidic pH; (b) raising the pH of the solution to form a reconstituted three-dimensional extracellular collagen matrix; (c) contacting at least a portion of the reconstituted three-dimensional extracellular collagen matrix with a photoactivating reagent; (d) removing excess photoactivating reagent; (e) irradiating the reconstituted three-dimensional extracellular collagen matrix, in a hydrated state without bubbling air or oxygen into the reaction mixture or vigorous stirring, using a source of light of sufficient energy to form a crosslinked scaffold; and (f) dehydrating the crosslinked scaffold.