Patent ID: 7183084
Filing Date: 2007-02-27
Classification: C12Q

Abstract:
1. A method for the amplification of a target nucleic acid region in a sample comprising the step of amplifying a known amount of a control nucleic acid and said target nucleic acid, wherein said control nucleic acid is different from said target nucleic acid and comprises at least one contiguous sequence at least 8 nucleotides in length essentially parallel complementary to said target nucleic acid region or to the complementary strand of said target nucleic acid region, wherein the amplified nucleic acids are detected in the amplification reaction mixture during the amplification reaction using a control probe and a different target probe, wherein the hybridization of the probes to the amplified nucleic acids during the amplification reaction is monitored and the control probe detects the amplified control nucleic acid and the target probe detects the amplified target nucleic acid, and wherein-at least one contiguous sequence of at least 8 nucleotides of the control probe is more than 80% parallel complementary to, and does not hybridize to: