Patent ID: 8778649
Filing Date: 2014-07-15
Classification: C12N,C12P,C12Y

Abstract:
1. A method for hydrolyzing lignocellulose, the method comprising contacting lignocellulose with a recombinant endo-beta-1,4-xylanase comprising an amino acid sequence with at least 90% sequence identity to SEQ ID NO: 1, wherein the lignocellulose is contacted with the recombinant xylanase at a temperature of at least 80° C. and a pH range from 3.0 to 9.0, wherein said xylanase comprises a Glu at a position corresponding to position 142 of SEQ ID NO: 1 and a Glu at a position corresponding to position 259 of SEQ ID NO: 1, wherein said xylanase comprises at least one peptide having the sequence Pro-Xaa 1 -Pro-Xaa 2 -Pro (SEQ ID NO: 40), wherein Xaa 1 and Xaa 2 are each independently selected from the group consisting of no amino acid, any one amino acid, any two amino acids, and any three amino acids, wherein said at least one peptide is at least 80% sequence identical to the peptide of SEQ ID NO: 27, and wherein said at least one peptide is located between the Glu at the position corresponding to position 142 of SEQ ID NO: 1 and the Glu at the position corresponding to position 259 of SEQ ID NO: 1.