Patent ID: 8426569
Filing Date: 2013-04-23
Classification: C07H,C12Q

Abstract:
1. A method for replicating a nucleic acid containing an unnatural base pair, comprising performing a nucleic acid replication reaction on a template strand which is a nucleic acid containing a nucleotide having a base of formula I below: (hereinafter referred to as Ds), and/or a base of formula II below: wherein R is —X—Y, or R is or R is wherein n is an integer selected from 1 to 12; m is an integer selected from 1 to 12; l is an integer selected from 1 to 12; X is selected from a group consisting of —C≡C—CH2—, —C≡C—, —C═C—, aryl, thienyl, imidazolyl, and thiazolyl; Y is selected from a group consisting of —CH3, —C2H5, —NH2, —OH, —COOH, —CHO, —SH, a substituted or unsubstituted aryl, —NHCO—Z, —CONH—Z, —NHCONH—Z, —O—Z, —COO—Z, —O—C(═O)—Z, —CO—Z, and —S—Z, wherein Z is selected from a group consisting of a fluorescent dye, biotin, an antibody-binding compound, a photocrosslinker, a chelating agent, an amino acid and a peptide, using a replication substrate containing the substituted or unsubstituted deoxyribonucleoside 5′-triphosphate having the base of formula II when the template strand contains a nucleotide having the base Ds, and using a replication substrate containing the substituted or unsubstituted deoxyribonucleoside 5′-triphosphate having the base Ds when the template strand contains a nucleotide having a base of formula II, thereby replicating a nucleic acid containing an unnatural base pair of the base Ds and a base of Formula II above.