Patent ID: 6670177
Filing Date: 2003-12-30
Classification: A61K,A61P,C07K,C12N

Abstract:
A method for obtaining a transduced population of viable cells by a retrovirus, comprising:infecting the cells with a retrovirus in the presence of an effective immobilized amount of fibronectin, a fibronectin fragment, or a mixture thereof to increase the efficiency of transduction of the cells by the retrovirus, said fibronectin, fibronectin fragment, or mixture thereof including a ligand which binds to the cells and a ligand which binds to the retrovirus, so as to co-localize the retrovirus and the cells, said infecting being conducted in a medium essentially free from a polycationic agent which increases the efficiency of transduction of the cells by the retrovirus in co-culture, but which agent reduces the efficiency of transduction of the cells by the retrovirus in the presence of said fibronectin, fibronectin fragment, or mixture thereof.