Patent ID: 6187553
Filing Date: 2001-02-13
Classification: C12Q,G01N

Abstract:
A method for eliminating physiological effects of heparin in a mixture of blood components in a blood collection tube having an inner wall, comprising the following steps:(a) preparing an additive formulation comprising heparinase, trehalose and a buffer;(b) spray coating said additive formulation onto the inner wall of said blood collection tube;(c) drying said additive formulation by applying an airjet or forced air to said inner wall of said blood collection tube at about 25 to about 30.degree. C. from about 5 to about 10 minutes;(d) vacuum drying said inner wall of said blood collection tube for about 2 hours;(e) removing oxygen from said inner wall of said blood collection tube by back flushing said blood collection tube with a gaseous mixture of CO.sub.2 and(f) stoppering said blood collection tube;(g) irradiating said blood collection tube at about 1.5 Mrads;(h) adding a blood sample containing heparin into said blood collection tube;(i) mixing said blood sample in said blood collection tube by manually inverting said blood collection tube about 5 to 10 times; and(j) allowing said blood sample to clot.