Patent ID: 6150165
Filing Date: 2000-11-21
Classification: A01N,A61K,C07K,C12N,C12R

Abstract:
An isolated polynucleotide that encodes a .delta.-endotoxin active against a coleopteran pest, wherein said polynucleotide hybridizes with a 285 bp probe obtainable by PCR amplification, with the primer shown in SEQ ID NO: 2 and with the reverse primer shown in SEQ ID NO: 3, of cellular DNA from E. coli NM522 having Accession No. NRRL B-21018; wherein hybridization occurs at 42.degree. C. in 50% formamide, 5X SSPE, 5X Denhardt's solution, 2% SDS, with 100 .mu.g/ml single stranded DNA; and wash occurs at 42.degree. C. in 2X SSC and 0.1% SDS.