Patent ID: 9127261
Filing Date: 2015-09-08
Classification: C12N,C12P

Abstract:
1. A method for controlling, in a cultivation of at least one of microbial prokaryotic cells or microbial eukaryotic cells, the growth of said cells in a single-phase, liquid culture medium to high cell densities by an enzyme-based fed-batch technique, wherein a metabolically inactive water soluble substrate-polymer or substrate-oligomer is degraded by the controlled addition of an enzyme to release the growth-limiting substrate-monomer in a controlled way into the single-phase, liquid culture medium, wherein if the cells grow slowly and the oxygen level in the culture medium stays high more enzyme is added and if the oxygen content in the culture medium becomes so low that anaerobic conditions occur no further enzyme is added, wherein the substrate-polymer or substrate-oligomer is present in an amount of 10 to 50 g/1.