Patent ID: 6812024
Filing Date: 2004-11-02
Classification: A61K,C07K

Abstract:
A method of manufacture of an anti-paratopic antibody comprising the steps of:(i) selecting from a pool of antibodies prepared from a plurality of humans, a prototypic set wherein members of the prototypic set are antibodies which bind HIV; (ii) subdividing the prototypic set selected in step (i) into antibody classes IgG, IgA, IgM, IgD and IgE; (iii) screening the antibody classes from step (ii) to select one or more classes which bind at least one of the HIV proteins selected from gp120, gp41, p24, p18, p55 and gp160; (iv) introducing one or more of the antibody classes selected in step (iii) into a host of a different species from humans to produce antibodies having characteristics which are anti-paratopic with respect to said introduced antibody and which are a synthetic replicate of the specific antigen or group of antigens used in step (iii); and (v) selecting, isolating and purifying the anti-paratopic antibodies produced in step (iv) which bind antibodies that bind HIV.