Patent ID: 6218104
Filing Date: 2001-04-17
Classification: C12Q

Abstract:
A method for distinguishing between high risk strains 16 and 33 or 18 of human papillomavirus and low risk strains 6 and 11 of human papillomavirus in a sample of human cervical tissue comprising:a) obtaining DNA from the human cervical tissue sample,b) simultaneously or individually adding oligonucleotide primer pairs which are specific for the E6 region of i) HPV 16 and 33, ii) HPV 18 or iii) HPV 6 and 11,c) amplifying the HPV E6 region by polymerase chain reaction using the E6 specific primer pair(s) andd) detecting the presence or absence of amplification products specific for HPV16 and 33 or 18 or HPV 6 and 11 to thereby distinguish between the presence of high risk strains HPV 16 and 33 or 18 and low risk strains HPV 6 and 11.