Patent ID: 7175985
Filing Date: 2007-02-13
Classification: C12Q,Y02A

Abstract:
1. A method for detecting mutations and/or polymorphisms within a specific region of a target nucleotide sequence comprising the steps of: (1) incubating the following elements (a) to (e) under conditions that allow complementary strand synthesis wherein second and first primers are used as origins: (2) correlating the amount of product synthesized by a complementary strand synthesis reaction, which uses the target nucleotide sequence as the template, with the presence of a mutation and/or polymorphism in the specific region, wherein a nucleotide sequence arranged on at least one of the 5′-ends of the second and first primer contains a nucleotide sequence that is complementary to the predicted nucleotide sequence of said specific region or the complementary strand thereof, and wherein the complementary strand, that is synthesized using the nucleotide sequence arranged on the 5′-end as the template and functions as the origin of complementary strand synthesis by annealing to the specific region or the complementary strand thereof, inhibits the complementary strand synthesis when the nucleotide sequence that contains the specific region is not a predicted one.