Patent ID: 6476203
Filing Date: 2002-11-05
Classification: A61K

Abstract:
A process for producing Icariin (ICN), which used for treating infertility and increasing immune function comprises:a. extracting said Epimedium brevicornum Maxim., Epimedium sagittatum Maxim; Epimedium pubescens Maxim., Epimedium wushanense T. S. Ying, and Epimedium Koreanum Nakai with 95% of ethanol; b. the ethanol extraction was recovered and the residue obtained; c. the residue extracted by 95% of petroleum ether, CH2Cl2, acetic ether and n-butyl alcohol sequentially; d. n-butyl alcohol extraction was recovered and residue obtained; e. the residue was passed through a column of polyamide and elute with ethanol; f. 10% of ethanol elute was passed through a column of silica gel and elute with CHCl3â€”CH3OH3; g. the CCl3â€”CH3OH solution concentrated under reduced pressure and the crystal was obtained; and h. the crystal was recrystalized from CH3OH, and i. the final product was Icariin.