Patent ID: 8614069
Filing Date: 2013-12-24
Classification: A61K,A61P,C07K

Abstract:
1. A polypeptide, comprising: a) a non-cytotoxic protease selected from the group consisting of Clostridial neurotoxin protease and Neisseria IgA protease, wherein the non-cytotoxic protease is capable of cleaving a SNARE, protein; b) a translocation peptide that is capable of translocating said non-cytotoxic protease from within an endosome of a mammalian cell, across the endosomal membrane thereof and into the cytosol of the mammalian cell, wherein the translocation peptide is selected from the group consisting of: a clostridial neurotoxin translocation domain selected from the group consisting of Botulinum Type A, B, C, D, F, F and G neurotoxin translocation domain and tetanus neurotoxin translocation domain, diphtheria toxin translocation domain, c) an epidermal growth factor (EGF) mutein, wherein said EGF mutein comprises an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO: 11, and wherein said EGF mutein retains the binding ability of SEQ ID NO: 11 to bind to an EGF receptor.