Patent ID: 6610485
Filing Date: 2003-08-26
Classification: C07K,C12N

Abstract:
A method for isolating or identifying a gene encoding a membrane-bound protein, the method comprising the steps of(i) introducing into cells a vector comprising a sequence comprising (a) a DNA encoding a secretable protein and (b) a cDNA ligated downstream of the secretable protein-encoding DNA, wherein the secretable protein can bind to an antigen and is selected from the group consisting of an antibody, a fragment of an antibody, and a single-chain antibody; (ii) expressing in the cells a fusion protein encoded by the sequence comprising (a) and (b); (iii) contacting the cells of (ii) with the antigen; (iv) selecting a cell that binds to the antigen; and (v) isolating or identifying the cDNA comprised in the vector that was introduced into the selected cell.