Patent ID: 9187729
Filing Date: 2015-11-17
Classification: A61K,A61P,C12N

Abstract:
1. A method for preparing a dried composition, said method comprising the steps of: (a) preparing an aqueous solution or dispersion comprising Venezuelan equine encephalitis virus or Venezuelan equine encephalitis virus replicon particles, 10 mM sodium phosphate, 4% (w/v) sucrose, 0.1% (w/v) human serum albumin, 100 mM sodium sulfate, 0.25% (w/v) glycerol, a buffer comprising boric acid and a pharmaceutically acceptable tetraborate salt for maintaining the pH of said aqueous solution or dispersion at 7.4; and (b) drying the aqueous solution or dispersion to obtain a dried composition containing Venezuelan equine encephalitis virus or Venezuelan equine encephalitis virus replicon particles that are dispersed in an amorphous glassy matrix comprising the sucrose, human serum albumin and sodium sulfate, wherein infectivity titer of the dried composition is no more than one log less than the infectivity titer of the aqueous solution.