Patent ID: 8986974
Filing Date: 2015-03-24
Classification: C12N,C12P

Abstract:
1. An isolated mutant strain, of a parent Trichoderma strain comprising a polynucleotide encoding a polypeptide and a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the first subtilisin-like serine protease gene, the first aspartic protease gene, the trypsin-like serine protease gene, the second subtilisin-like serine protease gene, and the second aspartic protease gene are modified rendering the mutant strain at least 95% deficient in the production of each of the first subtilisin-like serine protease, the first aspartic protease, the trypsin-like serine protease, the second subtilisin-like serine protease, and the second aspartic protease, respectively, compared to the parent wherein the first subtilisin-like serine protease is selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO: 2 or amino acids 20 to 882 of SEQ ID NO: 2; and (b) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to SEQ ID NO: 1 or nucleotides 58 to 2774 of SEQ ID NO: 1, or the cDNA thereof; wherein the first aspartic protease is selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO: 4 or amino acids 21 to 407 of SEQ ID NO: 4; and (b) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to SEQ ID NO: 3 nucleotides 61 to 1299 of SEQ ID NO: 3, or the cDNA thereof; wherein the trypsin-like serine protease is selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO: 6 or amino acids 20 to 259 of SEQ ID NO: 6; and (b) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to SEQ ID NO: 5 or nucleotides 58 to 930 of SEQ ID NO: 5, or the cDNA thereof; wherein the second subtilisin-like serine protease is selected from the group consisting of: (a) a polypeptide comprising of an amino acid sequence having at least 95% sequence identity to SEQ ID NO: 100 or amino acids 16 to 540 of SEQ ID NO: 100; and (b) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to SEQ ID NO: 99 or nucleotides 46 to 1681 of SEQ ID NO: 99 or the cDNA thereof; wherein the second aspartic protease is selected from the group consisting of: (a) a polypeptide comprising an amino acid sequence having at least 95% sequence identity to SEQ ID NO: 108 or amino acids 18 to 395 of SEQ ID NO: 108; and (b) a polypeptide encoded by a polynucleotide comprising a nucleotide sequence having at least 95% sequence identity to SEQ ID NO: 107 or nucleotides 52 to 1339 of SEQ ID NO: 107, or the cDNA thereof; and wherein the polypeptide is native or foreign to the parent