Patent ID: 6846636
Filing Date: 2005-01-25
Classification: C07K

Abstract:
1. A method of screening a substance for the ability to modulate high density lipoprotein (HDL) holoparticle binding and/or internalization activity of an isolated mammalian receptor which specifically binds an HDL holoparticle, comprising: a) contacting the substance with a cell producing a functional HDL receptor, and b) assaying the cell for a modulation of the HDL holoparticle binding and/or internalization activity of the receptor, whereby a modulation of the HDL holoparticle binding and/or internalization activity of the receptor identifies a substance with the ability to modulate the HDL holoparticle binding and/or internalization activity of the HDL receptor, wherein a functional HDL receptor comprises, as measured by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) under non-reducing conditions: (i) a subunit of approximately 450,600 kDa molecular weight, wherein the 450-600 kDa molecular weight subunit is cubilin, and one or more subunits selected from the group consisting of a subunit of approximately 40-50 kDa molecular weight, a subunit of approximately 120 kDa molecular weight and a subunit of approximately 400 kDa molecular weight; (ii) a subunit of approximately 800 kDa molecular weight and one or more subunits selected from the group consisting of a subunit of approximately 40-50-kDa molecular weight, a subunit of approximately 120 kDa molecular weight and a subunit of approximately 400 kDa molecular weight; (iii) a subunit of approximately 400 kDa molecular weight and one or more subunits selected from the group consisting of a subunit of approximately 40-50 kDa molecular weight and a subunit of approximately 120 kDa molecular weight; or (iv) a subunit of approximately 120 kDa molecular weight and a subunit of approximately 40-50 kDa molecular weight.