Patent ID: 6893667
Filing Date: 2005-05-17
Classification: C12Q,G01N

Abstract:
1. A method for profiling of protein extract from hyper acidic parts of scented Geranium plant ( Pelargonium sp.) said hyper acidic parts being of pH about 3.0, said method comprising: (a) homogenizing the hyper acidic parts of the plant in buffers comprising acidity-neutralizing salts and acidity sequestering compounds, (b) centrifuging the homogenate for about 30 minutes, (c) obtaining supernatant, from the centrifugation of part (b) wherein said supernatant is the protein extract (d) optionally centrifuging the supernatant of part (c) to obtain a protein sediment and estimating the amount of protein sediment obtained, (e) calculating enzymatic activity and enzyme-kinetics of peroxidase in said extract of part (c) spectrophometerically, (f) mixing the protein extract of step (c) with sodium dodecyl sulfate-polyacrylamide (SDS) sample buffer, (g) boiling the mixture of part (f) for a time ranging between 1-10 minutes, (h) cooling the boiled mixture immediately, (i) electrophorizing the mixture part (h) using sodium dodecyl sulfate-polyacrylamide polyacrylamide gel electrophoresis (SDS-PAGE), (j) determining polypeptide profiles of the electrophorized mixture using molecular weight markers, (k) electrophorizing the mixture of step (h) using polyacrylamide gel electrophoresis and using a catalysis specific chromogenic reaction mixture, (I) visualizing catalytic activity bands, and (m) determining enzyme patterns of said protein extract.