Patent ID: 9175340
Filing Date: 2015-11-03
Classification: C12N,C12Q

Abstract:
1. A method for interaction-dependent polymerase chain reaction, comprising: (i) providing a nucleic acid template, wherein the nucleic acid template comprises: (ii) contacting the nucleic acid template with a first primer, wherein the first primer comprises: (iii) incubating the nucleic acid template contacted with the first primer under conditions suitable for the candidate ligand to bind to the target molecule with a K (iv) incubating the nucleic acid template contacted with the first primer under conditions suitable for bound first primer to hybridize with the first primer hybridization site of the nucleic acid template it is bound to and for primer extension, and not allowing for efficient primer site hybridization and primer extension of the first primer not connected to the nucleic acid template by an interaction characterized by a K (v) contacting the nucleic acid template contacted with the first primer with a PCR primer complementary to the second primer hybridization site and a PCR primer complementary to the third primer hybridization site or with a PCR primer complementary to the second and third primer hybridization site; and (vi) performing a polymerase chain reaction to amplify the nucleic acid template or a fragment thereof.