Patent ID: 6194145
Filing Date: 2001-02-27
Classification: C12Q,Y02A

Abstract:
A method of amplifying a nucleic acid of the genus legionella, comprising:(a) hybridizing two nucleic acid primers that are genus-specific for legionella with the legionella nucleic acid, to form a hybrid, wherein each of the two nucleic acid primers independently comprises a sequence of at least 15 bases, which sequence is, in direct order, at least 90% identical to a portion of SEQ ID NO: 1, or a complement thereof, which portion contains a same number of bases as the sequence;(b) extending the nucleic acid primers of the hybrid to form extension products using the legionella nucleic acid as a template;(c) separating the extension products and the legionella nucleic acid; and(d) repeating steps (a)-(c) at least once to amplify the nucleic acid of the genus legionella.