Patent ID: 6897035
Filing Date: 2005-05-24
Classification: C12Q,Y10S

Abstract:
1. A method for the detection or determination of histamine in a sample of microdialysates or dialysates, comprising the steps of: (A) applying said sample to a biosensor comprising an electrode and (i) a mono-enzyme system of an amine oxidase, which is a copper-containing grass pea oxidase (E. C. 1.4.3.6) or (ii) a bi-enzyme system of an amine oxidase, which is a copper-containing grass pea oxidase (E.C. 1.4.3.6), coupled with horseradish, soybean, tobacco, sweet potato or palmtree peroxidase; wherein said electrode is a carbon/graphite based electrode, and whereby said amine oxidase is cross-linked to the electrode into an osmium based redox polymer; and (B) detecting an electrical output from said biosensor.