Patent ID: 9068191
Filing Date: 2015-06-30
Classification: C12N

Abstract:
1. A method of transiently expressing one or more nucleotide sequences in one or more cells of an intact whole soybean plant, comprising: a) contacting the surface of an intact soybean leaf of a whole soybean plant with an abrasive; b) contacting the abraded soybean leaf and/or the whole soybean plant comprising the leaf with a solution comprising bacterial cells competent for nucleic acid transfer and a surfactant, wherein the bacterial cells comprise the one or more nucleotide sequence(s) to be transiently expressed, and wherein said bacterial cells are c) applying a vacuum to the contacted soybean leaf of step (b) and/or the whole soybean plant comprising said leaf of step (b); and d) releasing the vacuum, thereby introducing the solution into the abraded soybean leaf, whereby the one or more nucleotide sequences are transiently expressed in one or more cells of the soybean plant.