Patent ID: 8828718
Filing Date: 2014-09-09
Classification: A61K,C12N

Abstract:
1. An integrating gene transfer viral vector (IGTV) which comprises at least one genetic insulator element (GIE), wherein said at least one GIE comprises at least two copies of an element selected from the group consisting of (A) a CTF binding site; and (B) a sequence consisting of a first CTCF binding site and a second CTCF binding site: wherein the CTF binding site is selected from the group consisting of SEQ ID NO:26, SEQ ID NO:28, SEQ ID NO:29, and SEQ ID NO:30; and wherein said first and said second CTCF binding sites are from regulatory sequences of different genes and wherein said first and second CTCF binding sites comprise a different sequence selected from the group consisting of SEQ ID:7, SEQ ID NO:8, SEQ ID NO:27, and SEQ ID NO:31, wherein the at least one GIE is positioned in the vector such that following genomic insertion of the IGTV, the at least one GIE is positioned between a genomic sequence and the coding sequence of the IGTV.