Patent ID: 7842477
Filing Date: 2010-11-30
Classification: A61K,A61P,C12N,C12P,C12Y

Abstract:
1. A method for producing a protein, the method comprising: (a) providing a recombinant host cell containing a first nucleic acid comprising a first promoter operably linked to a nucleotide sequence encoding a protein requiring gamma-carboxylation and a second heterologous nucleic acid comprising a second promoter operably linked to a nucleotide sequence encoding a γ-glutamyl carboxylase, wherein the first promoter is stronger than the second promoter; (b) culturing the cell under conditions suitable for expressing both nucleic acids, wherein the protein requiring gamma-carboxylation and the γ-glutamyl carboxylase are expressed in a ratio of at least 10:1 and the protein requiring gamma-carboxylation is carboxylated in the cell, thereby producing a gamma-carboxylated protein; (c) isolating the gamma-carboxylated protein or an activated form thereof; and (d) preparing a pharmaceutical composition comprising the isolated gamma-carboxylated protein or an activated form thereof.