Patent ID: 7169561
Filing Date: 2007-01-30
Classification: C12Q

Abstract:
1. A method of determining a target polynucleotide comprising; providing a target polynucleotide, and a probe set, hybridizing the first probe one and the second probe to the target polynucleotide; ligating the first probe to the second probe to form a first ligation product; dissociating the first ligation product from the target polynucleotide; hybridizing the single-stranded stem portion of the first probe to the single-stranded stem portion of the second probe to form a self-complementary ligation product; hybridizing the protruding tail of the self-complementary ligation product to the protruding tail of the hairpin linker; ligating the self-complementary ligation product to the hairpin linker to form a loop ligation product; detecting the loop ligation product; and, determining the target polynucleotide.