Patent ID: 6372424
Filing Date: 2002-04-16
Classification: C07H,C07K,C12N,C12Q

Abstract:
A method of comparing cleavage products comprising the steps of:a) providing: i) an enzymatic cleavage means; ii) a test nucleic acid substrate containing sequences from one or more microorganisms; and iii) control cleavage products produced by cleavage of a reference nucleic acid from a reference microorganism; b) treating said test nucleic acid substrate under conditions such that said substrate forms one or more cleavage structures, said cleavage structures comprising one or more intra-strand secondary structures, wherein a primer is not annealed to said cleavage structures; c) reacting said cleavage means with said cleavage structures so that one or more test cleavage products are produced; and d) comparing said test cleavage products to said control cleavage products.