Patent ID: 9187540
Filing Date: 2015-11-17
Classification: A61K,A61P,C07K

Abstract:
1. A method for ameliorating hypertension in a subject in need thereof, the method comprising; (a) transforming an endothelial cell in vitro with an expression vector comprising a nucleic acid molecule encoding an E2F2 polypeptide fused to a protein transduction domain, wherein the protein transduction domain is selected from the group consisting of an HIV-TAT protein, a unidecapeptide protein transduction domain (YGRKKRRQRRR (SEQ ID NO: 2)), a polyarginine sequence, a VP22 domain, and a antennapedia protein transduction domain, wherein the E2F2 fusion polypeptide retains E2F2 activity; (b) expressing the E2F2 polypeptide in the endothelial cell; (c) isolating the E2F2 polypeptide; and (d) administering the E2F2 polypeptide to the subject, thereby ameliorating the hypertension.