Patent ID: 7807419
Filing Date: 2010-10-05
Classification: C12P,Y02E

Abstract:
1. A process for producing high-sugar content hydrolysate from biomass comprising: a) providing a portion of reaction components in a vertical stirred tank reactor having a particle size reduction mechanism, said reaction components comprising: b) reacting said slurry and enzymes under suitable conditions; c) applying the particle size reduction mechanism; d) adding an additional portion of pretreated biomass producing a higher solids biomass slurry; e) optionally adding an additional portion of (ii) or another saccharification enzyme consortium comprising cellulases, hemicellulases, or mixtures thereof; f) reacting said higher solids biomass slurry under suitable conditions; and g) optionally repeating one or more steps (c), (d), (e), and (f) one or more times, whereby a high sugar content hydrolysate is produced, wherein the concentration of total soluble sugars produced is at least about 100 g/L, and wherein the yield stress of the slurry is maintained at less than 30 Pa.