Patent ID: 6964844
Filing Date: 2005-11-15
Classification: C07D,G01N,Y10T

Abstract:
1. A method for determining the presence or amount of at least one target ligand in a fluid sample, the method comprising: a. contacting a fluid sample suspected of containing said target ligand with a ligand analogue conjugate and a ligand receptor, said ligand analogue conjugate comprising at least one ligand analogue coupled to a signal development element comprising a water soluble hybrid phthalocyanine derivative, to form a homogeneous reaction mixture, whereby said ligand analogue conjugate competes with said target ligand for binding to said ligand receptor, wherein said water soluble hybrid phthalocyanine derivative is a tetraazapyrrole molecule, wherein (i) at least one of the four pyrrole moieties is fused to a single carbocyclic ring to form a phthalocyanine subunit, (ii) each of the other three pyrrole moietie is fused to between zero and three carbocyclic rings to form a subunit selected from the group consisting of an azaporphine subunit, a phthalocyanine subunit, a naphthalocyanine subunit and an anthranylocyanine subunit, and (iii) at least two of the four pyrrole moieties comprises a different number of carbocyclic rings fused thereto; b. generating a detectable signal from ligand analogue conjugate bound to said ligand receptor in said reaction mixture; and c. relating the detectable signal to the presence or amount of said target ligand in said fluid sample.