Patent ID: 6080585
Filing Date: 2000-06-27
Classification: B01J,C07H,C12N,C12Q,C40B,Y10T

Abstract:
A method of investigating the intramolecular structure of a polynucleotide, which method comprises:providing a polynucleotide, said polynucleotide having a known sequence and having an intramolecular structure, and providing a plurality of different oligonucleotides in the form of an array on a solid surface, all of said different oligonucleotides being fully complementary to different segments of the polynucleotide;applying the polynucleotide in solution to the array of oligonucleotides under hybridisation conditions which preserve the intramolecular structure of the polynucleotide; andobserving quantitative differences between interactions of the polynucleotide with different oligonucleotides of the array, said differences between interactions being indicative of the intramolecular structure of the polynucleotide.