Patent ID: 8975033
Filing Date: 2015-03-10
Classification: G01N

Abstract:
1. A method for the diagnosis of severe rheumatoid arthritis (RA) in a subject comprising: a) obtaining a sample comprising blood, plasma or serum from a subject suspected of having severe RA; b) providing a substrate having a first capture probe bound thereto, wherein the capture probe comprises peptidyl arginine deiminase 3 (PAD3) protein or a portion or fragment thereof which comprises an antigen recognized by autoantibodies present in the serum of subjects suffering from RA; c) contacting the substrate having the capture probe bound thereto with the sample from the subject; d) measuring the amount of a complex of the capture probe and the autoantibodies formed by step c); e) providing a normal reference level sample comprising blood, plasma or serum; f) comparing the amount of a complex of the capture probe and the autoantibodies formed from the subject suspected of having severe RA to the amount of a complex of the capture probe and the autoantibodies formed from the normal reference level sample; and g) identifying the subject suspected of having severe RA as having severe RA when the amount of a complex of the capture probe and the autoantibodies formed from the subject suspected of having severe RA is statistically significant increased compared to the amount of a complex of the capture probe and the autoantibodies formed from the normal reference level sample.