Patent ID: 6987000
Filing Date: 2006-01-17
Classification: C07K,C12N,C12Q,G01N

Abstract:
1. A method for identifying a compound which modulates the kinase activity of a polypeptide selected from the group consisting of: (a) an isolated polypeptide which is encoded by the nucleotide sequence contained in the plasmid deposited with ATCC as Accession Number PTA-1530; (b) an isolated cardiac-related ankyrin-repeat protein kinase polypeptide comprising an amino acid sequence encoded by a nucleic acid molecule which hybridizes to a complement of a nucleic acid molecule consisting of SEQ ID NO:1 or 3 in 6×SSC at 45° C., followed by one or more washes in 0.2×SSC, 0.1% SDS at 65° C.; (c) an isolated cardiac-related ankyrin-repeat protein kinase polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is at least 90% identical to the nucleotide sequence of SEQ ID NO:1 or 3; (d) an isolated cardiac-related ankyrin-repeat protein kinase polypeptide comprising an amino acid sequence which is at least 90% identical to the amino acid sequence of SEQ ID NO:2; and (e) an isolated polypeptide comprising amino acid residues 463-716 of SEQ ID NO:2; the method comprising: (i) contacting the polypeptide or a cell expressing the polypeptide with a test compound; and (ii) determining the effect of the test compound on the activity of the polypeptide; to thereby identify a compound which modulates the kinase activity of the polypeptide.