Patent ID: 9115382
Filing Date: 2015-08-25
Classification: A61K,C12Q,G01N

Abstract:
1. A kit for detecting an exotoxin produced by beta-hemolytic streptococcus bacterium in a biological sample of saliva collected from a subject comprising: a sample-receiving cup or swab for collecting the saliva; a substrate cleaved by the biological fluid collected from the subject to induce a discernable color change discernable to an unaided human eye, said substrate is selected from the group consisting of D-Val-Leu-Lys-p-Nitroanilide Dichloride, pyroGlu-Phe-Lys-p-Nitroanilide Hydrochloride, pyroGlu-Leu-Lys-p-Nitroanilide Hydrochloride, H-D-Ala-Leu-Lys-fluca 1-2Galβ1-4Glc-7-amino-4-methyl-coumarin, H-D-Val-Leu-Lys-fluca 1-2Galβ1-4Glc-7-amino-4-methyl-coumarin, N-succinyl Phe-Ala-p-Nitroanilide, Leu-p-Nitroanilide, amido-methylcoumarin, and cholesterol-containing membrane, said cholesterol-containing membrane comprising a lipid bilayer, a cholesterol, and a chromophore; an exotoxin protein present in the biological fluid and selected from the group consisting of streptokinase, streptolysin O, streptolysin S, and streptodornase; plasminogen; an enzyme inhibitor inhibiting rogue protein present in the biological fluid and not preventing the exotoxin protein produced by the beta-hemolytic streptococcus bacterium from cleaving said substrate to prevent a false positive result of said color change; and a reference card showing positive and negative control results and having instructions for the use thereof for detecting the exotoxin associated with the presence of the beta-hemolytic streptococcus in the biological sample.