Patent ID: 6187287
Filing Date: 2001-02-13
Classification: A61K,A61P,C07K

Abstract:
A humanized LL2 (hLL2) monoclonal antibody (mAb) or fragment thereof comprising the complementarity-determining regions (CDRs) of mouse LL2 (mLL2) and the framework (FR) regions of the light and heavy chain variable regions of a human antibody and the light and heavy chain constant regions of a human antibody, wherein said hLL2 mAb or fragment thereof retains substantially the B-lymphoma cell and leukemia cell targeting and cell internalization characteristics of said mLL2 mAb, and wherein said hLL2 mAb or fragment thereof is less immunogenic in a human subject than is said mLL2 mAb, wherein the CDRs of the light chain variable region of mLL2 comprise CDR1 comprising amino acids 24 to 40 of SEQ ID NO: 2, CDR2 comprising amino acids 56 to 62 of SEQ ID NO: 2 and CDR3 comprising 95 to 103 of SEQ ID NO: 2 and the CDRs of the heavy chain variable region of mLL2 comprise CDR1 comprising amino acids 31 to 35 of SEQ ID NO: 4, CDR2 comprising amino acids 50 to 66 of SEQ ID NO: 4 and CDR3 comprising 99 to 105 of SEQ ID NO: 4.