Patent ID: 7928205
Filing Date: 2011-04-19
Classification: A61K,A61P,C07K

Abstract:
1. A method of producing an IgG2 antibody preparation enriched for one of several IgG2 structural variants which differ by disulfide connectivity in the hinge region, comprising contacting a preparation of an IgG2 antibody that has been recombinantly produced by mammalian cells with a reduction/oxidation coupling reagent at a pH of about 5 to about 11 in the absence of or the presence of a chaotropic agent, wherein (i) said IgG2 antibody to be subjected to the method elutes as several separate forms, corresponding to said several IgG2 structural variants, on RP-HPLC, (ii) said contacting in the absence of a chaotropic agent enriches one of said several IgG2 structural variants, and (iii) said contacting in the presence of a chaotropic agent enriches another of said several IgG2 structural variants.