Patent ID: 8691508
Filing Date: 2014-04-08
Classification: C12Q

Abstract:
1. A method of assisting execution of a multiplexed diagnostic assay on a solid phase at high signal-to-noise ratio, comprising: providing a plurality of first pairs of oligonucleotides suitable for amplification of a first nucleic acid that is suitable as a diagnostic marker for a first condition; providing a plurality of separate first labeled oligonucleotides, wherein each of the first labeled oligonucleotides has a common first portion that is suitable for hybridization with the first nucleic acid, and wherein each of the first labeled oligonucleotides has a distinct second portion; wherein each of the first labeled oligonucleotides has a unique identifier associated with the distinct second portion; wherein each of the first labeled oligonucleotides has a structure of5′-(X) providing instructions to separately perform a plurality of amplification reactions in a plurality of distinct patient samples, wherein each reaction uses one pair of the plurality of first pairs of oligonucleotides and one labeled oligonucleotide of the plurality of first labeled oligonucleotides; wherein the amplification reaction is performed using a DNA polymerase having 5′->3′-exonuclease activity under conditions that allow removal of (a) a quencher from the labeled oligonucleotide or (b) the distinct second portion from the labeled oligonucleotide; providing instructions to pool the plurality of amplification reactions, and to hybridize the pooled labeled oligonucleotides to a solid phase using the respective second portions; and providing a deconvolution table that associates a single test result for each of the different patient samples based on the first diagnostic marker and the unique identifier.