Patent ID: 7195891
Filing Date: 2007-03-27
Classification: C12Q

Abstract:
1. A method for assaying a myeloperoxidase in a sample, said method comprises: a) contacting a sample suspected of containing a myeloperoxidase with serine, hydrogen peroxide, and chlorine to generate glycolaldehyde; b) converting said glycolaldehyde generated in step a) to glycolate in the presence of a glycolaldehyde converting enzyme and a first electron acceptor in its oxidized form; c) converting said glycolate from step b) to glyoxylate in the presence of a second electron acceptor in its oxidized form and a glycolate converting enzyme, whereby reduced form of the second electron acceptor is generated; said glyoxylate is converted back to glycolate in the presence of a third electron acceptor in its reduced form and a glyoxylate converting enzyme to form a cycling reaction system, whereby oxidized form of the third electron acceptor is generated; wherein the second electron acceptor and the third electron acceptor are different; and d) assessing concentration change of the reduced or oxidized form of the second electron acceptor or the reduced or oxidized form of the third electron acceptor in said cycling reaction system, whereby the presence, absence and/or the amount of the myeloperoxidase in the sample is determined.