Patent ID: 7122337
Filing Date: 2006-10-17
Classification: A61K,A61P,C12Q,Y10S

Abstract:
1. A method of determining the calciotropic activity of a preparation of algae of the genus Padina pavonica, said preparation selected from the group consisting of an extract of said algae and a powder of said algae, said method consisting of culturing human or animal osteoblasts in a culture medium rich in calcium ions in a well plate, adding to said culture medium said extract or a powder of said algae Padina pavonica and culturing between 24 to 72 hours, thereafter eliminating non-fixed calcium ions in the culture medium, acidifying the culture medium to destroy extracellular matrix formed by the osteoblasts, thereafter filtering the culture medium to remove solid material, and determining concentration of fixed calcium ions in the osteoblasts in comparison with a calibration scale of a known activator of the fixation of calcium or in the presence of an inhibitor of the fixation of calcium or both.