Patent ID: 8895025
Filing Date: 2014-11-25
Classification: A61K,C07K,C12N,G01N

Abstract:
1. An isolated DNA molecule comprising a DNA sequence encoding an infectious RNA molecule encoding a Porcine Reproductive and Respiratory Syndrome (PRRS) virus, wherein said encoding DNA sequence is selected from the group consisting of: (a) SEQ ID NO:1; (b) a sequence that has at least 70% identity to SEQ ID NO:1; and (c) a sequence that hybridizes to the complement of SEQ ID NO:1 under highly stringent conditions which comprise hybridization to filter bound DNA in 0.5M NaHPO wherein, in all cases, said isolated DNA molecule (1) lacks an encoding DNA sequence corresponding to all of amino acids 616 through 752 (SEQ ID NO: 1 positions 3186-3596) or all of amino acids 628 through 759 (SEQ ID NO: 1 positions 3222-3617) in the hypervariable region of the nonstructural protein 2-coding region of PRRS ORF1a; (2) wherein said isolated DNA molecule does not comprises a nucleotide sequence, within said PRRS encoding sequence, that encodes a detectable heterologous antigenic epitope; and (3) wherein the DNA sequence than encodes the infectious RNA molecule is operably linked to a promoter.