Patent ID: 8119572
Filing Date: 2012-02-21
Classification: C12Q,C40B,G01N

Abstract:
1. A method of determining the binding specificity of a protein for a differentially covalently modified peptide comprising: a) contacting a combinatorial library with a protein to allow binding of the protein to the combinatorial library, the combinatorial library comprising covalently modified peptides, the covalently modified peptides comprising histone H4 N-terminal peptides consisting of at least 5 amino acids of the following structure: (SEQ ID NO: 71) wherein each peptide has the same amino acid sequence and at least two covalent modifications, wherein the covalently modified peptides are attached to a plurality of solid phase supports such that each solid phase support has a covalently modified peptide having the same set of covalent modifications attached thereto, b) detecting the protein bound to the covalently modified peptides using a label; and c) determining the binding specificity of the protein for the covalently modified peptides.