Patent ID: 8889383
Filing Date: 2014-11-18
Classification: C12N,C12P,C12Y,Y02E

Abstract:
1. Recombinant cells with increased production of isoprene, the cells comprising one or more heterologous nucleic acids comprising nucleotide sequences selected from the group consisting of: a. a mevalonate E (mvaE) gene and a mevalonate S (mvaS) gene from b. a mvaE gene and a mvaS gene from c. a mvaE gene and a mvaS gene from d. a mvaE gene and a mvaS gene from wherein said mvaE gene and mvaS gene encode polypeptides having thiolase, HMG-CoA synthase, and HMG-CoA reductase catalytic activities, and wherein the cells further comprise: i. one or more nucleic acids encoding polypeptides of the lower MVA pathway; and ii. a heterologous nucleic acid encoding an isoprene synthase polypeptide, wherein the cells produce increased amounts of isoprene compared to isoprene-producing cells that (A) contain an mvaE gene and mvaS gene from Enterococcus faecalis ( E. faecalis ) and (B) do not contain said mvaE gene and mvaS gene from E. gallinarum, E. casseliflavus, E. faecium , or L. grayi.