Patent ID: 7772203
Filing Date: 2010-08-10
Classification: A61P,C12N,Y02A

Abstract:
1. A method of enhancing RNA silencing of a target mRNA, the method comprising: (a) selecting a target sequence in an mRNA expressed in a mammalian cell or plant cell; and (b) contacting the mammalian cell or the plant cell with an RNAi agent, the RNAi agent comprising an antisense strand that is complementary to the target sequence, wherein at least one nucleotide within 5 or fewer nucleotides from the 3′ end of the antisense strand is substituted with a nucleotide that forms a G:U wobble base pair when the antisense strand is base paired with the target sequence, wherein the mammalian cell or plant cell is contacted under conditions such that RNA silencing of the target mRNA is enhanced, and wherein the RNAi agent is chemically or enzymatically synthesized.