Patent ID: 8927287
Filing Date: 2015-01-06
Classification: C12M

Abstract:
1. A method of dispersion of assemblies of biological material selected from plant tissue, assemblies of cultured plant cells and assemblies of plant embryogenic mass suspended in a liquid into smaller assemblies of biological material, said method including at least one dispersion sequence, which comprises the following steps: i) providing a disperser comprising a flow channel arranged in a loop configuration for re-circulation of the assemblies to be dispersed in the flow channel, the flow channel including at least two constrictions wherein longitudinal axes of said at least two constrictions align with each other, such that assemblies of biological material flowing through the flow channel are first subjected to axially extensional strain and radially compressional strain, and then to axially compressional strain and radially extensional strain from fluid dynamics forces; ii) using the disperser to subject the assemblies of biological material to fluid dynamics forces causing axially extensional strain and radially compressional strain; iii) using the disperser to subject the assemblies of biological material to fluid dynamics forces causing axially compressional strain and radially extensional strain from fluid dynamics forces; and iv) repeating steps ii) and iii) in sequence until the assemblies of biological material are dispersed into smaller assemblies of biological material.