Patent ID: 9133500
Filing Date: 2015-09-15
Classification: C07K,C12N,C12Q,G01N

Abstract:
1. A method to identify antigen-binding moieties which bind to an epitope of a polypeptide, wherein said polypeptide comprises an enzymatic activity, said method comprising (a) screening a library of antigen-binding moieties against a polypeptide comprising the epitope and having the enzymatic activity and isolating those members of said library that bind to said polypeptide, (b) counter-screening the members of the library isolated in step (a) against a variant of the polypeptide, wherein said variant polypeptide comprises a mutated epitope and is devoid of enzymatic activity, and (c) isolating those members that do not bind to said variant polypeptide, therein identifying those members that bind to the epitope of the polypeptide.