Patent ID: 8663616
Filing Date: 2014-03-04
Classification: A61K,A61P,A61Q

Abstract:
1. A method comprising 1) providing a set of reaction components comprising: b) a source of peroxygen; and c) an enzyme catalyst having perhydrolytic activity, wherein said enzyme catalyst comprises a fusion protein comprising the following general structure: PAH-[L]y-OCBD or OCBD-[L]y-PAH wherein PAH is an enzyme having perhydrolytic activity; wherein said enzyme is a carbohydrate esterase having a CE-7 signature motif that aligns with a reference sequence SEQ ID NO: 2, said signature motif comprising: i) an RGQ motif at positions corresponding to positions 118-120 of SEQ ID NO:2; ii) a GXSQG motif at positions corresponding to positions 179-183 of SEQ ID NO:2; and iii) an HE motif at positions corresponding to positions 298-299 of SEQ ID NO;2; OCBD is a peptidic component having affinity for oral cavity surface; wherein the peptidic component is a single chain variable fragment (scFv) antibody or a single chain polypeptide lacking an immunoglobulin fold; L is a peptide linker ranging from 1 to 100 amino acids in length; and y is 0 or 1; and 2) combining the reaction components of (1) under suitable reaction condition whereby at least one peracid is enzymatically produced; and 3) contacting an oral cavity surface with the at least one peracid whereby the oral cavity surface receives a peracid-based benefit selected from the group consisting of bleaching, teeth whitening, disinfecting, destaining, deodorizing, decreasing or removing biofilm, and combinations thereof.