Patent ID: 6143577
Filing Date: 2000-11-07
Classification: B03C,G01N,Y10S

Abstract:
A process for separating cells, comprising:contacting a sample containing (1) target cells which are affixed to paramagnetic balls, (2) free cells, and (3) surplus paramagnetic balls, with a nonhomogeneous magnetic field,wherein, during the contacting, the sample is circulated in a tube, wherein the nonhomogeneous magnetic field is applied over a section of the tube measuring less than the length of the tube, and wherein the tube has a proximal portion and a distal portion with respect to fluid flow in the tube,wherein the surplus paramagnetic balls are immobilized in the proximal portion of the tube and the target cells which are affixed to paramagnetic balls are immobilized in the distal portion of the tube.