Patent ID: 6602662
Filing Date: 2003-08-05
Classification: C12Q,G01N,H01J,Y10T

Abstract:
A process for determining whether a target nucleotide is present in a nucleic acid molecule, comprising:a) hybridizing a nucleic acid molecule with a primer oligonucleotide that is complementary to a sequence that is adjacent to a region suspected of containing the target nucleotide, thereby producing a hybridized primer; b) contacting the hybridized primer with i) deoxyribonucleoside triphosphates, ii) chain terminating nucleotides selected from the group consisting of dideoxyribonucleoside triphosphates and 3â€²-deoxynucleoside triphosphates, and iii) a DNA polymerase, whereby the hybridized primer is extended until a chain terminating nucleotide is incorporated, thereby producing an extended primer; and c) determining the molecular mass of the extended primer, thereby determining whether the target nucleotide is present in a nucleic acid molecule.