Patent ID: 8658396
Filing Date: 2014-02-25
Classification: C12Q,G01N

Abstract:
1. A method for detecting a known protein variant, wherein the sequence of the protein variant to be detected is known, in a sample selected from the group consisting of a whole blood sample and a dried blood spot sample, comprising: (i) directly digesting protein in the selected sample to produce a defined series of peptides in admixture; (ii) directly ionising the defined series of peptides in the admixture to form an admixture of ionised species and identifying 1 to 20 ionised species of known mass/charge ratio indicative of the protein variant by mass spectrometry scanning of a limited window of mass/charge ratio selected according to the known sequence of the variant peptide; and (iii) directly subjecting the admixture of ionised species comprising the identified ionised species to collision induced dissociation to form derived ionised peptide species and detecting a derived ionised peptide species of known mass/charge ratio that confirms the presence of the protein variant identified in step (ii) in the sample.