Patent ID: 7846731
Filing Date: 2010-12-07
Classification: B82Y,C12M,C12N

Abstract:
1. A method of introducing a nucleic acid into cells by electroporation, comprising the step (a) of providing an electrode with a cationic surface; the step (b) of adsorbing and loading a nucleic acid onto the cationic surface of an electrode; the step (c) of allowing cells to adhere onto the surface of the nucleic acid-loaded electrode obtained in the step (b); and the step (d) of applying electric pulses to the cells, wherein the electrode with a cationic surface is an electrode on which a monolayer of a thiol, disulfide or sulfide compound having an anionic functional group at the terminal is formed and a cationic polymer is adsorbed onto the surface of the monolayer.