Patent ID: 6022728
Filing Date: 2000-02-08
Classification: A61K,Y10S

Abstract:
A method for producing a disrupted whole cell vaccine from cells of Actinobacillus pleuropneumoniae for injection into swine to produce immunity which comprises:(a) isolating cells of a strain of the Actinobacillus pleuropneumoniae from a swine infected with the strain which are virulent as a result of passage of the strain through the first swine;(b) growing the strain which is isolated in a growth medium;(c) harvesting the virulent cells of the strain from the growth medium;(d) disrupting the harvested virulent cells of the strain using a French press which disrupts the cells by a sudden reduction of pressure on the growth medium in an aqueous solution to obtain a mixture containing disrupted cells, capsular and subcapsular antigens; and(e) preserving the mixture with a preservative which kills the Actinobacillus pleuropneumoniae to provide said vaccine.