Patent ID: 8211423
Filing Date: 2012-07-03
Classification: A61K,A61P,C12N

Abstract:
1. A method of improving left ventricular contractility in a mammalian subject in need thereof comprising; (a) isolating skeletal muscle cells from a human subject, (b) suspending the human skeletal muscle cells in a medium in a first cell culture container for between 30 and 120 minutes thereby producing a cell population of adherent cells and a population of non-adherent cells; (c) decanting the medium and the population of non-adherent cells from the first cell culture container to a second cell culture container; (d) allowing the population of the decanted non-adherent cells in the medium from the first cell culture container to attach to the walls of the second cell culture container for 1-3 days; (e) isolating the population of cells adhered from the walls of the second cell culture container, wherein the isolated population of cells are muscle-derived progenitor cells (MDCs); and (f) administering the MDCs to the heart of the mammalian subject; thereby, improving left ventricular contractility of the heart.