Patent ID: 8669109
Filing Date: 2014-03-11
Classification: C12N

Abstract:
1. A method of producing a protein in Chinese hamster ovary (CHO) cells which contain a gene which codes for the protein under serum-free conditions, comprising: (a) growing the CHO cells which contain a gene which codes for the protein under serum-free conditions which allow replication of the CHO cells; (b) depositing fewer than five of the CHO cells in a cell culture vessel under serum-free conditions; (c) replicating the suitably deposited CHO cells in the presence of 100 to 10,000 irradiated autologous feeder cells per ml medium under serum-free conditions, wherein the feeder cells are non-adherent feeder cells adapted to serum-free medium; (d) cultivating the replicated cells under serum-free conditions in which the gene of interest is expressed; and (e) isolating and purifying the gene product from the cells or the culture supernatant.