Patent ID: 8188230
Filing Date: 2012-05-29
Classification: C07K,G03C

Abstract:
1. A process for producing a collagen-like polypeptide, wherein said process comprises the following steps: (a) expressing a nucleic acid sequence encoding said collagen-like polypeptide in a fungus host; (b) growing said fungus in culture medium; and (c) purifying said collagen-like polypeptide from said culture medium; wherein said collagen-like polypeptide comprises consecutive [Gly-X-Y] repeats; wherein Gly stands for glycine, X and Y represent any amino acid; wherein the length of the consecutive [Gly-X-Y] repeat being such that its weight on amino acid basis is at least 2.5 kDa; wherein the amino acid sequence of said collagen-like polypeptide comprises more than 4 different amino acids; and wherein said collagen-like polypeptide is free of all the sequences LXXR (SEQ ID NO:51), IXXR (SEQ ID NO:52), VXXR (SEQ ID NO:53), MXXR (SEQ ID NO:54), and MGPR (SEQ ID NO:14), and wherein said purified collagen-like polypeptide is free of helix structure.