Patent ID: 8252563
Filing Date: 2012-08-28
Classification: C12N,C12P

Abstract:
1. An isolated DNA comprising any nucleotide sequence of the following a) to d): a) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:1; b) a nucleotide sequence of a DNA: i) wherein the DNA has at least 90% sequence homology with a DNA comprising a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:1, and ii) wherein the nucleotide sequence encodes an amino acid sequence of a protein having the ability to asymmetrically reduce an ortho-substituted phenylglyoxalic acid compound to produce corresponding optically active ortho-substituted mandelic acid; c) a nucleotide sequence of a DNA: i) wherein the DNA hybridizes under a stringent condition including washing for 30 minutes at 65° C. in 0.1×SSC with a DNA comprising a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:1, and ii) wherein the nucleotide sequence encodes an amino acid sequence of a protein having the ability to asymmetrically reduce an ortho-substituted phenylglyoxalic acid compound to produce corresponding optically-active ortho-substituted mandelic acid compound; and d) the nucleotide sequence of SEQ ID NO:2.