Patent ID: 8580499
Filing Date: 2013-11-12
Classification: B01J,G01N

Abstract:
1. An apparatus for detection and/or quantification of different target molecules being bound at different locations of a surface of an optically transparent solid support, said apparatus comprising: (a) an optically transparent solid support having refractive index n1 that comprises (i) at least four target molecules bound on capture molecules present at defined locations on said solid support surface in the form of an array, (ii) a side which is inclined relative to the surface of the support on which the target molecules are bound and, (iii) a chamber being formed on the surface of the solid support forming a device covering the bound capture molecules and wherein the refractive index of the solid support is higher than 1.33, (b) a light source to produce a light beam that reaches the surface of the support having immobilized capture molecules with an angle of 90° plus or minus 10°, said light beam being capable of homogeneous excitation of the surface of the support on which the target molecules are bound, (c) a lens located on the emitted light path between the surface of the support on which the target molecules are bound and a detector, and (d) a detector, positioned at an observation angle θ