Patent ID: 9157067
Filing Date: 2015-10-13
Classification: C12N

Abstract:
1. A method of differentiating mouse or rat Epiblast Stem Cells (EpiSCs) into cells of a mesoderm lineage comprising culturing the mouse or rat EpiSCs in a chemically defined medium (CDM) supplemented with Activin, FGF2 and BMP4 such that the mouse or rat EpiSCs differentiate into cells of a mesoderm lineage, wherein the mouse or rat EpiSCs: (a) express Oct4, nanog, Sox2, FGF5 and nodal and do not express Blimp1, Stella, alkaline phosphatase, GBX2 or Rex1; and (b) do not maintain pluripotency in the presence of leukemia inhibitory factor (LIF); and wherein the mouse EpiSCs are obtained from the late epiblast layer of a post-implantation mouse embryo and the rat EpiSCs are obtained from the late epiblast layer of a post-implantation rat embryo.