Patent ID: 7405060
Filing Date: 2008-07-29
Classification: A61K,A61P,C07K,C12N

Abstract:
1. A method of producing a transformed cell that co-expresses a combination of V H and V L regions to form a multivalent composition from a subject's B-cell lymphoma cells, comprising: a) providing: b) isolating nucleic acid from said malignant cells, said nucleic acid comprising nucleotide sequences selected from the group consisting of nucleotide sequences encoding at least one V c) inserting said nucleic acid comprising nucleotide sequences encoding said V d) introducing said pluralities of said first and second expression vectors and said amplification vector into said parent cell line to generate transformed cells, wherein a ratio ranging from 2:20 to 2:50 of said amplification vector to said first or second expression vector is employed; e) introducing said transformed cells into a first aqueous solution, said first aqueous solution comprising an inhibitor capable of inhibiting said first inhibitable enzyme, wherein the concentration of said inhibitor present in said first aqueous solution is sufficient to prevent growth of said parent cell line; and f) identifying a transformed cell capable of growth in said first aqueous solution, wherein said transformed cell capable of growth expresses a combination of V wherein said multivalent composition comprises said expressed combination of V H and V L regions.