Patent ID: 6821511
Filing Date: 2004-11-23
Classification: A61K,C07K,C12N

Abstract:
A process for transducing a cell with a DNA sequence, which process comprises(1) contacting said cell with genetic material coding for products that do not generate viral particles, wherein said genetic material consists essentially of (a) a first genetic construct for integration into a chromosome of said cell, said first genetic transcript being free of DNA encoding adeno-associated virus rep protein and including, in a 5â€² to 3â€² direction, (i) a first adeno-associated viral ITR, (ii) a promoter controlling a DNA encoding a polypeptide or genetic transcript of interest, (iii) a DNA encoding a polypeptide or genetic transcript of interest, and (iv) a second adeno-associated viral ITR, and (b) a second genetic construct that provides, upon expression, an adeno-associated virus rep protein in trans with respect to said first genetic construct, wherein said cell is transduced with said first genetic construct and said second genetic construct upon contact of said cell with said first genetic construct and said second genetic construct and wherein said adeno-associated virus rep protein is the only adeno-associated viral protein expressed by said second genetic construct, and (2) introducing said cell into a mammal.