Patent ID: 6515120
Filing Date: 2003-02-04
Classification: C12Q

Abstract:
A method for sequencing a DNA or RNA molecule comprising the steps of separating a terminal monomer from the DNA or RNA molecule, contacting the separated terminal monomer with an aptamer that is 120 nucleotides or less and comprises a sequence selected from the group consisting of:(a) 5â€²-CGGRGGAGGNACGGRGGAG-3â€² (SEQ ID NO:1), wherein R is G or A and N is T, C, A or G; (b) 5â€²-GGGAGGGTN1N2N3GGN4G-3â€² (SEQ ID NO:2), wherein N1, N2, N3, and N4 is any monomer selected from the group consisting of A, C, G and T; (c) 5â€²-GGT N1N2N3GGN4G-3â€² (SEQ ID NO:3), wherein N1, N2, N3, and N4 is any monomer selected from the group consisting of A, C, G and T; and (d) 5â€²-TGGGN1TGGGN2N3TGGGN4AGGGT-3â€² (SEQ ID NO:4), wherein N1, N2, and N4 is any monomer selected from the group consisting of A, C, G and T and N3 is no monomer or any monomer selected from the group consisting of A, C, G and T; and determining whether the aptamer of subsections (a) to (d) bound to the terminal monomer.