Patent ID: 7674620
Filing Date: 2010-03-09
Classification: A61P,C12N

Abstract:
1. A method of generating a differentiated neural cell population from primate pluripotent stem cells comprising the following steps: (a) expanding a culture of primate pluripotent stem cells; (b) culturing the pluripotent stem cells to select for neuroprogenitor cells that are positive for nestin; (c) sorting the nestin-positive neuroprogenitor cells for enrichment of NCAM-positive cells; (d) differentiating the nestin-positive, NCAM-positive cells to generate a differentiated neural cell population by culturing the cells in a differentiation media which comprises N-acetyl cysteine and TGF-β3 or interleukin-1β or both, wherein the differentiated neural cell population comprises at least about 60% dopaminergic neurons or at least about 30% serotonergic neurons.