Patent ID: 8273954
Filing Date: 2012-09-25
Classification: C12N

Abstract:
1. A method for genetically transforming dicotyledonous plant cells which comprises contacting dicotyledonous plant cells, which are susceptible to genetic transformation by Agrobacterium cells, with Agrobacterium cells containing a chimeric Ti plasmid comprising a T-DNA region which region comprises in sequence, (i) a first (ii) a chimeric gene which functions in said plant cells comprising a 5′ promoter region from a gene which exists naturally in a plant cell or naturally enters a plant cell, a structural coding sequence encoding a polypeptide expressible in said plant cells and a 3′ non-translated region encoding a polyadenylation signal, said promoter and 3′ non-translated region being operably linked to said structural coding sequence; and (iii) a second said T-DNA border sequences enabling the transfer and incorporation of T-DNA into the genome of said plant cell and said T-DNA region including the T-DNA border sequences containing no plant tumorigenic genes.