Patent ID: 7595172
Filing Date: 2009-09-29
Classification: C07K

Abstract:
1. A method for making an acylated human GLP-1 polypeptide, said method comprising the steps of: (i) culturing a host cell comprising a polynucleotide sequence encoding a precursor molecule of said human GLP-1 polypeptide under suitable conditions for expression of said precursor molecule, wherein said precursor molecule comprises said human GLP-1 polypeptide and an N-terminal extension, said N-terminal extension being cleavable from the polypeptide at a lysine cleavage site; (ii) expressing said precursor molecule; (iii) separating said expressed precursor molecule from said culture; (iv) preferentially acylating the ε-amino group of at least one lysine residue in said precursor molecule without acylating the ε-amino group of the lysine residue of said lysine cleavage site, to produce an acylated precursor molecule; and (v) removing the N-terminal extension from said acylated precursor molecule by enzymatic cleavage to produce said acylated human GLP-1 polypeptide, wherein said acylated human GLP-1 is Arg 34 GLP1 (7-37) acylated in position Lys 26 .