Patent ID: 6368838
Filing Date: 2002-04-09
Classification: B01J,B81B,B81C,B82Y,C12N,C12Q,C40B

Abstract:
A method of forming a patterned surface of at least one first population of cells from a mixture including the first population of cells and at least one second population of cells different from the first population of cells, comprising the steps of:forming on a surface of a plate a plurality of discrete cytophilic regions, each of which promote adherence of the at least one first population of cells, arrayed in a predetermined geometric pattern, the cytophilic regions being isolated one from another by cytophobic regions which do not promote adherence of cells; contacting the surface with the mixture; and selectively adhering only cells of the at least one first population of cells to the cytophilic regions to yield a predetermined geometric pattern of a plurality of cells, each of which regions includes the at least one first population of cells adhered thereto and is essentially free of the at least one second population of cells, the regions with cells adhered thereto being isolated one from another by regions that are essentially free of cells.