Patent ID: 8389793
Filing Date: 2013-03-05
Classification: A01K,A61K,C07K,C12N

Abstract:
1. A method of producing a monoclonal antibody that specifically binds to an epitope on a molecule of interest, wherein the epitope is not present naturally in a wild-type murine host, comprising the steps of: immunizing a knockout murine host with an antigen that comprises the epitope present on said molecule of interest in a manner sufficient for said knockout murine host to develop antibodies against said antigen, wherein said knockout murine host comprises a knockout mutation in an enzyme involved in a post-translational modification of a polypeptide or a modification of a lipid; and wherein in the wild-type murine host the modified portion of the polypeptide or lipid is exposed on a cell surface or exposed on a secreted molecule; isolating the splenocytes from said immunized knockout murine host and fusing said splenocytes with immortalized cells to form a hybridoma; and assaying said hybridoma to determine if it produces the monoclonal antibody, wherein when the wild-type murine host is immunized with the antigen it does not produce a monoclonal antibody that binds to the epitope, and wherein said knockout murine host is selected from a mouse having a knockout mutation in mannosyl (α1,6-)-glycoprotein β1,6-N-acetyl-glucosaminyltransferase V (MGAT5; GNT-V), a mouse having a knockout mutation in core 2 β1-6N-acetylglucosaminyltransferase (C2 GlcNAcT), and a mouse having a knockout mutation in β(1,4)-galactosyltransferase 1 (β4GalT-I).