Patent ID: 8426153
Filing Date: 2013-04-23
Classification: C07K,C12Q,G01N

Abstract:
1. A biosensor comprising: a fluorogen-activating peptide having an active domain; and a blocking peptide linked to the fluorogen-activating peptide through a peptide linker comprising an amino acid sequence that is specifically recognized by furin; wherein one of the fluorogen-activating peptide and the blocking peptide comprises a variable heavy chain domain of an antibody and the other peptide comprises a variable light chain domain of a different antibody; wherein the blocking peptide associates with the fluorogen-activating peptide thereby blocking the active domain of the fluorogen-activating peptide when the peptide linker is intact, and wherein the fluorogen-activating peptide and the blocking peptide at least partially disassociate when the peptide linker is cleaved, thereby allowing the fluorogen-activating peptide to interact with a cognate fluorogen and modulate a fluorescence signal.