Patent ID: 9193984
Filing Date: 2015-11-24
Classification: C07K,C12N,C12Q,G01N

Abstract:
1. A method for detecting interactions of a bait and a prey comprising the steps of: (a) preparing a cell comprising (b) allowing the bait of the first construct and the prey of the second construct to interact; (c) treating with a signaling material to translocate the first construct to the plasma membrane of the cell; and (d) detecting the translocation of the first construct and the second construct to the plasma membrane of the cell in the cell, wherein the translocation module is protein kinase C or its variants, wherein protein kinase C or its variants comprise an amino acid sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5 and SEQ ID NO:7.