Patent ID: 9029092
Filing Date: 2015-05-12
Classification: C09B,C12Q,G01N

Abstract:
1. A solution phase assay method for an analyte in a sample, the solution phase assay method comprising: a) forming an aqueous reaction mixture comprising the following components: wherein by adding the components, in any order or concurrently, to an aqueous solution, b) adding to the aqueous reaction mixture a trigger solution comprising a peroxide compound, wherein the trigger solution releases a detectable chemiluminescent signal in the presence of the selective signal inhibiting agent that is correlated to the amount of the analyte-bound chemiluminescent-labeled specific binding partner and the analyte-bound activator-labeled specific binding partner in the aqueous reaction mixture, and wherein the selective signal inhibiting agent causes the ratio of signal produced by reaction between the chemiluminescent label compound and the activator label compound in the complex with the analyte to exceed the signal from reaction between the chemiluminescent label compound and the activator label compound when not in such a complex.