Patent ID: 7326579
Filing Date: 2008-02-05
Classification: G01N

Abstract:
1. A particle agglutination immunoassay method of quantifying an antigen in a sample of whole blood, comprising the steps of: providing a sample of the whole blood; adding a hemolysis reagent to the sample of whole blood for hemolysing blood corpuscles in the sample of whole blood and enabling a subsequent immunoreaction; adding a latex reagent, including insoluble latex carriers onto which antibodies specifically reacting with the antigen in the sample of whole blood have been immobilized, to the hemolysed whole blood and to provide an agglutination reaction with the hemolysed whole blood sample thereby forming an agglutination reaction product wherein the antigen in the hemolysed whole blood sample reacts with the antibodies immobilized on the insoluble carriers to provide the agglutination reaction product; irradiating the agglutination reaction product in the hemolysed whole blood sample with radiation wavelength of approximately 700 nm to 1000 nm which is substantially free from absorption by both hemoglobin and the hemolysis reagent; and measuring, only with the wavelength of approximately 700 nm to 1000 nm, a change in absorbance of the incident radiation by the agglutination reaction product to determine the quantity of antigens in the sample.