Patent ID: 7445885
Filing Date: 2008-11-04
Classification: H01J

Abstract:
1. A process for analyzing enzyme-catalyzed conversions of nonpolymeric substrates to nonpolymeric products, which comprises analyzing a substrate or a product or a substrate and product of the enzyme-catalyzed conversion with the aid of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) mass spectrometry, with said process comprising the following steps: a) enzymatically converting a nonpolymeric substrate to a nonpolymeric product; and b) analyzing the substrate or product or the substrate and product during or after the enzyme-catalyzed conversion of step (a) using MALDI-TOF mass spectrometry, wherein the analysis is carried out on a polished or coated carrier material having a roughness of Rz>I and wherein the substrate or product or the substrate and product are mixed with an internal standard, wherein nonpolymeric substrates and nonpolymeric products are not peptides, proteins, oligonucleotides, polynucleotides, oligosacoharides, polysacoharides, natural polymers or artificial polymers.