Patent ID: 6730521
Filing Date: 2004-05-04
Classification: G01N,Y10S,Y10T

Abstract:
A method of performing a non-separation assay for determining a level of binding of one component to another, the method comprising:providing a first labelled component in a solution; providing an array of beads, cells, surfaces or wells and placing a second component onto said beads or surfaces or into said cells or wells; immersing the array with the solution; scanning the array with an illuminating light beam such that the light passes through the solution whilst illuminating the beads, cells, surfaces or wells; determining an intensity of light received from each of the beads, cells, surfaces or wells and solution at at least one wavelength during illumination; determining from a peak intensity value of light received, an area beneath a plot of the peak intensity, or the area beneath a 3-D intensity plot of a second component location, an amount of binding of the first labelled component to the second component; determining from pre- and post-second component intensity values, the lowest intensity signal obtained during the scan of the array or an average background value obtained during the scan, an amount of first labelled component in the solution; and determining a ratio of i) an amount of binding of the first labelled component to the second component to ii) the amount of first labelled component in the solution.