Patent ID: 6673550
Filing Date: 2004-01-06
Classification: C07D,C07H,C07K,C12Q,C40B,G01N

Abstract:
A probe set for detecting the presence or absence of one or more target compounds, the probe set comprising a plurality of electrophoretic probes selected from the group defined by the formula:[(D, M)â€”L]kâ€”T wherein:T is a target-binding moiety specific for a target compound; k is an integer greater than or equal to one; L is a cleavable linkage that is cleaved by oxidation by an active species; D is a detection group; and M is a mobility modifier consisting of from 1 to 200 atoms other than hydrogen selected from the group consisting of carbon, oxygen, sulfur, nitrogen, phosphorus, and boron; wherein, upon cleavage of L, an eTag reporter comprising a detection group, D, and a mobility modifier, M, is produce having a molecular weight in the range of from 150 to 10,000 daltons and a distinct charge/mass ratio so that eTag reporters from different electrophoretic probes form resolved bands upon electrophoretic separation; and wherein detection group, D, is a fluorescent molecule selected from the group defined by the formula: wherein:Z is H, lower alkyl, substituted lower alkyl, lower alkenyl, substituted lower alkenyl, lower alkynyl, substituted lower alkynyl, cycloalkyl, alkoxy, substituted alkoxy, phenoxy, substituted phenoxy, aromatic, substituted aromatic, phenyl, substituted phenyl, polycyclic aromatic, substituted polycyclic aromatic, heterocyclic, substituted heterocyclic, chlorine, fluorine, bromine, iodine, COOH, carboxylate, amide, nitrile, nitro, sulfonyl, sulfate, sulfone, amino, tethered amino, quaternary amino, imino, phosphorus containing species, or polymer chains of from about 2 to about 10 monomer units, A is O, N+(R1)(R2) wherein R1 and R2 are independently H, lower alkyl, or substituted lower alkyl, D is OH, OR3 wherein R3 is lower alkyl, substituted lower alkyl, aryl, substituted aryl, N(R1)(R2) wherein R1 and R2 are independently H, lower alkyl, or substituted lower alkyl, W1, W2, W3, W4, W5 and W6 are independently H, lower alkyl, substituted lower alkyl, lower alkenyl, substituted lower alkenyl, lower alkynyl, substituted lower alkynyl, cycloalkyl, alkoxy, substituted alkoxy, phenoxy, substituted phenoxy, aromatic, substituted aromatic, phenyl, substituted phenyl, polycyclic aromatic, substituted polycyclic aromatic, heterocyclic, substituted heterocyclic, chlorine, fluorine, bromine, iodine, COOH, carboxylate, amide, nitrile, nitro, sulfonyl, sulfate, sulfone, amino, tethered amino, quaternary amino, or imino, X1-X4 are independently H, lower alkyl, substituted lower alkyl, lower alkenyl, substituted lower alkenyl, lower alkynyl, substituted lower alkynyl, cycloalkyl, alkoxy, substituted alkoxy, phenoxy, substituted phenoxy, aromatic, substituted aromatic, phenyl, substituted phenyl, polycyclic aromatic, substituted polycyclic aromatic, heterocyclic, substituted heterocyclic, chlorine, fluorine, bromine, iodine, COOH, carboxylate, amide, nitrile, nitro, sulfonyl, sulfate, sulfone, amino, tethered amino, quaternary amino, or imino, wherein W2 and W3 may be taken together to form one or more rings comprising 4 to 14 atoms and comprising 1 to 7 unsaturations, and wherein W4 and W5 may be taken together to form a ring comprising 4 to 14 atoms and comprising 1 to 7 unsaturations.