Patent ID: 6800451
Filing Date: 2004-10-05
Classification: C07F,G01N,Y10T

Abstract:
A method for detecting the presence or concentration of glucose in a sample which may also contain an alpha-hydroxy acid or a beta-diketone, which comprises:a) exposing the sample to a compound having at least two recognition elements for glucose, oriented such that the interaction between the compound and glucose is more stable than the interaction between the compound and the alpha-hydroxy acid or beta-diketone, said compound also containing a detectable moiety having a detectable quality that changes in a concentration-dependent manner when said compound is exposed to glucose in said sample; and b) measuring any change in said detectable quality to thereby determine the presence or concentration of glucose in said sample, wherein the presence of the alpha-hydroxy acid or the beta-diketone does not substantially interfere with said determination, wherein the compound is selected from the grout consisting of: 9-[N-(2-boronobenzyl)-N-[3-(methacrylamido) propylamino]methyl]-10-[N-(2-boronobenzyl)-N-[6-(cyclohexanecarboxamido) hexylamino]methyl]anthracene; 9-[N-(2-boronobenzyl)-N-[3-(methacrylamido)propylamino]-methyl]-10-[N-(2-boronobenzyl)-N-[2-(carboxyethyl)amino]methyl]-anthracene; 9-[N-(2-boronobenzyl)-N-[3-(methacrylamido)prorylamino]-methyl]-10-(N-(2-boronobenzyl)-N-[3-(N-6-(9-anthracenecarbox-amido) hexylamino carbonyl) ethylamino]methyl]anthracene; 9-[N-(2-boronobenzyl)-N-[3-(methacrylamido)propylamino]-methyl]-10-[N-(2-boronobenzyl)-N-[6-(3-carboxypropionamido)-hexylamino]methyl]anthracene; N-(3-Methacrylamidopropyl)-4-[2-N-[[2-(borono)benzyl]-[6-(N-[2-(borono)benzyl]-6-N-(3-carboxypropanamidoethyl)aminohexyl]]-aminoethylamino]naphthalene-1,8-dicarboximide; N-Butyl-4-[2-N-[[2-(borono)benzyl]-[6-(N-[2-(borono)benzyl]-6-N-(2-methacrylamidoethyl) aminohexyl]]aminoethylamino]-naphthalene-1,8-dicarboximide; and salts thereof.