Patent ID: 7326530
Filing Date: 2008-02-05
Classification: A61P,C12N,C12Q,G01N

Abstract:
1. A method for localizing a polypeptide on a surface of a cytoplasmic membrane, characterized in that the method comprises: (I) preparing a construct for introducing into a cell for localizing a heterogeneous polypeptide on a cell surface, which comprises one nucleotide sequence selected from the group consisting of the following (A) to (C): wherein a nucleic acid encoding a heterogeneous polypeptide to be expressed is operably linked downstream of one nucleotide sequence selected from the group consisting of said (A) to (C) via a nucleotide sequence for operably linking the nucleotide encoding a heterogeneous polypeptide; (II) introducing the construct obtained in said step (I) into a host cell capable of adding an O-glycan thereto as a posttranslational modification to give a transformant harboring the construct; (III) culturing the host cell in said step (II) under conditions sufficient to express a polypeptide comprising the heterogeneous polypeptide and the polypeptide having an activity for targeting the heterogeneous polypeptide; and (IV) detecting the heterogeneous polypeptide on the surface of the cytoplasmic membrane.