Patent ID: 8017337
Filing Date: 2011-09-13
Classification: C12Q

Abstract:
1. A method of detecting methicillin-resistant S. aureus (MRSA) in a sample, wherein said sample contains one or a mixture of bacterial species, said method comprising: (a) providing a first set of primers, wherein said first set of primers are complementary to at least a portion of a mecA polynucleotide sequence; (b) providing a second set of primers, wherein said second set of primers are complementary to at least a portion of a bridging region; (c) providing a third set of primers, wherein said third set of primers are complementary to at least a portion of an (d) combining said first, second and third set of primers with said sample in a reaction mixture; (e) performing a multi-cycle amplification reaction with said reaction mixture; (f) determining cycle numbers of appearance of each of said mecA, bridging region and (g) comparing said cycle numbers of appearance of said mecA, bridging region, and wherein if said mecA polynucleotide sequence has a cycle number of appearance that is substantially the same as that of either said bridging region sequence or said