Patent ID: 8227218
Filing Date: 2012-07-24
Classification: C12N,C12P

Abstract:
1. A method for the enzymatic preparation of an alkenone of formula (2) from an α,β-unsaturated alkynone of formula (1) wherein R R comprising reducing the compound of formula (1) in the presence of: (i) a reductase comprising at least one polypeptide selected from the group consiting of SEQ ID NO: 1, 2, 3, 5, 7, and 9; or (ii) with a functionally equivalent reductase which has at least 80% sequence identity with SEQ ID NO: 1, 2, 3, 5, 7, and 9; wherein the reaction is carred out: (i) in an essentially ATP-free environment, wherein the reductase or functionally equivalent reductase is in the form of (a) a purified reductase, (b) an enriched reductase, or (c) a protein fraction comprising the reductase; or (ii) with a reconbinant bacterial microorganism that expresses the reductase or functionally equivalent reductase; and the reaction is carried out with NADPH or NADH as cofactor.