Patent ID: 6057096
Filing Date: 2000-05-02
Classification: A61K,A61P,C07C,C07D,C07H,C07K,C12P,C12Q,G01N

Abstract:
A method, comprising the steps of:a) conjugating a bioreactive agent to one or more oligonucleotide primers with a covalent bond that is selectively cleavable with electromagnetic radiation, wherein the conjugated bioreactive agent has a chemical structure selected from the group consisting of: ##STR7## wherein SUB comprises the oligonucleotide primer; R.sub.1 and R.sub.2 are selected from the group consisting of hydrogen, alkyls, substituted alkyls, aryls, substituted aryls, --CF.sub.3, --NO.sub.2, and --COOH, and may be the same or different; A is a divalent functional group selected from the group consisting of --O--, --S-- and --N--; Y comprises one or more polyatomic groups which may be the same or different; V comprises one or more monoatomic groups which may be the same or different; Q comprises a spacer moiety; m.sub.1 and m.sub.2 are integers between 1-5 which can be the same or different; and D comprises a selectively detectable moiety which is distinct from R.sub.1 and R.sub.2, wherein D is not --NO.sub.2 ;b) amplifying a nucleic acid sequence with said conjugated oligonucleotide primers; andc) treating said amplified sequences with electromagnetic radiation.