Patent ID: 8257939
Filing Date: 2012-09-04
Classification: C07C,C07D,C07F,C12N,C12Q,C12Y,G01N,Y10S

Abstract:
1. A method for detecting a mutant dehalogenase comprising: (a) contacting said mutant dehalogenase with a dehalogenase substrate, wherein said dehalogenase substrate comprises one or more functional groups, wherein said mutant dehalogenase comprises one or more amino acid substitutions relative to the corresponding wild-type dehalogenase, and wherein said one or more amino acid substitutions result in the mutant dehalogenase forming a stable covalent bond with said dehalogenase substrate; and (b) detecting the presence of said functional group thereby inferring the presence of said mutant dehalogenase based on the presence of said functional group.