Patent ID: 8586339
Filing Date: 2013-11-19
Classification: C12N,C12Y

Abstract:
1. A process comprising: (a) providing a microbial cell homogenate comprising soluble and insoluble components, wherein said microbial cell homogenate comprises a recombinantly-produced thermophilic enzyme having perhydrolytic activity; (b) subjecting the microbial cell homogenate to a first heat treatment period ranging from at least 4 hours but no more than 24 hours at a temperature ranging from 40° C. to 65° C.; (c) subjecting the heat-treated microbial cell homogenate from step (b) to a second heat treatment period ranging from 5 minutes to 4 hours at a temperature ranging from 75° C. to 85° C.; (d) removing the insoluble components from the microbial cell homogenate obtained after performing step (b) and step (c) by centrifugation or filtration to obtain a solution comprising the recombinantly produced thermophilic enzyme having perhydrolytic activity; and e) concentrating the solution comprising the thermophilic enzyme of step (d) by filtration, evaporation or a combination of protein precipitation and redissolution whereby a concentrate is obtained comprising the recombinant thermophilic enzyme having perhydrolytic activity.