Patent ID: 9045748
Filing Date: 2015-06-02
Classification: C12N,C40B

Abstract:
1. A method for expression screening of filamentous fungal transformants, comprising: (a) isolating single colony transformants of a DNA library of different plasmids introduced into (b) preparing each different plasmid from each of the single colony (c) introducing a sample of each of the different plasmid preparations of step (b) into separate suspensions of protoplasts of a filamentous fungus to obtain transformants thereof, wherein each transformant contains one or more copies of an individual and different plasmid from the DNA library; (d) growing the individual filamentous fungal transformants obtained from transformation of each of the different plasmid preparations of step (c) on growth medium that selects for filamentous fungal transformants, while suppressing growth of untransformed filamentous fungi, and also enables expression and secretion of each different polypeptide into the growth medium; and (e) measuring activity or a property of each different polypeptide encoded by each individual and different DNA secreted into the culture medium of step (d).