Patent ID: 6767703
Filing Date: 2004-07-27
Classification: C12N,C12Q

Abstract:
A method for detecting a target intermediate tandem repeat DNA sequence having a low incidence of stutter artifacts, comprising the steps of:(a) providing a sample of DNA having at least one target intermediate tandem repeat sequence, wherein the target intermediate tandem repeat sequence is a region of the DNA containing at least one repeat unit consisting of a sequence of five (5), six (6), or seven (7) base pairs repeated in tandem at least two (2) times; and (b) amplifying the target intermediate tandem repeat sequence using at least one oligonucleotide primer, comprising a sequence which is complementary to and flanks a region of a double-stranded DNA marker containing a template intermediate tandem repeat sequence, wherein the template intermediate tandem repeat sequence is a region of the DNA marker which contains the repeat unit sequence repeated in tandem at least two (2) times, provided that the DNA marker has a sequence of SEQ ID NO:32; and (c) detecting the target intermediate tandem repeat sequence in the sample of DNA, wherein an average stutter artifact of no more than 2.4% is observed.