Patent ID: 7582420
Filing Date: 2009-09-01
Classification: C12Q,Y10T

Abstract:
1. A method for detecting at least 100 different target nucleic acid sequences of interest in a sample, each sequence comprising from 3′ to 5′, contiguous first, second, and third target domains, wherein the first target domain has a detection position one, two, three or four nucleotides from the 3′ terminal base of the second target domain, and the second target domain is at least one nucleotide, comprising the steps of: (a) providing a sample having at least 100 different target nucleic acid sequences of interest that are immobilized on a solid support; (b) contacting the sample with a set of probes for each of the at least 100 different target nucleic acid sequences of interest to form hybridization complexes, each set comprising: (c) contacting the hybridization complexes with an extension enzyme and dNTPs, wherein for each hybridization complex, if the base at the interrogation position is perfectly complementary to the base at the detection position, then the first probe is extended along the second target domain; (d) ligating the extended first probes to second probes to form amplification templates; (e) amplifying the amplification templates with first and second universal primers to produce amplicons; (f) immobilizing the amplicons on solid phase capture probes that are specific to individual adapter sequences; and (g) detecting the presence of at least 100 different immobilized amplicons at the capture probes; thereby indicating of the presence of the at least 100 different target sequences of interest in the sample.