Patent ID: 6635418
Filing Date: 2003-10-21
Classification: C12Q

Abstract:
A method for determining total nucleic acid in a sample, which comprises:a) mixing at least one random primer at least 4 nucleotides in length, having at least one detectable species with a sample which may contain nucleic acid, b) adding at least one nucleotide triphosphate having at least one binding species and optionally at least one second nucleotide triphosphate, c) adding at least one nucleic acid polymerase, d) incubating the mixture of step c), under conditions which allow said at least one nucleic acid polymerase to be active, e) contacting the mixture of step d) with at least one solid phase, f) measuring total nucleic acid in said sample by measuring the total amount of said at least one detectable species bound to said solid phase, and g) determining whether the total nucleic acid in said sample is higher or lower than a threshold amount of contamination, wherein the threshold amount of contamination is equal to or less than 100 pg.