Patent ID: 6803226
Filing Date: 2004-10-12
Classification: A01G

Abstract:
A method of forming an artificial Shiro of Matsutake, comprising:the step of inducing growth of Matsutake hyphae by aseptically homogenizing a colony of Matsutake hyphae to substantially separate the hypha cells from each other without destroying the cells and aseptically culturing the obtained hyphae in a liquid nutrient medium containing a carbon source; the subsequent step of preparing an inoculum of Matsutake hyphae by aseptically replacing the liquid nutrient medium containing the growth-induced Matsutake hyphae with a liquid nutrient medium containing no carbon source after said growth inducing step; and the step of culturing aseptically the prepared inoculum of the Matsutake hyphae in a culture substrate in which Matsutake hyphae can grow containing a carbon source and at least one active principle selected from the group consisting of a surfactant and a natural vegetable oil.