Patent ID: 6376173
Filing Date: 2002-04-23
Classification: B01J,C12Q

Abstract:
A process for detecting the presence of different selected standard microorganisms in a sample by reverse genome probing, wherein said process comprises the steps of:(A) preparing a master soled surface having affixed thereto isolated spots of genomic deoxyribonucleic acid from each of said different selected standard microorganisms by: (i) obtaining an individual culture of each of said selected standard microorganisms from an environment of interest, (ii) isolating a genomic deoxyribonucleic acid preparation from each of said individual cultures, and (iii) affixing each of said genomic deoxyribonucleic acid preparation from step (Aii) to said master solid surface, (B) preparing a reverse genome probe from said sample by: (i) isolating a total nucleic acid preparation from said sample by: (ii) labelling said total nucleic acid preparation, (C) contacting said master solid surface from step (A) with said reverse genome probe from step (B) under conditions permitting hybridization, (D) washing said master solid surface resulting from step (C) so as to remove non-hybridized reverse genome probe, and (E) detecting hybridized reverse genome probe.