Patent ID: 6033849
Filing Date: 2000-03-07
Classification: C12N,C12Q

Abstract:
A method for isolating and identifying an extrachromosomal amplified target nucleic acid from micronuclei in a cell comprising:contacting a cell suspected of having extrachromosomal amplified target nucleic acid with a non-alkaloid agent capable of inducing the formation of micronuclei in the cell, under conditions sufficient to produce micronuclei;isolating the micronuclei from the cell;isolating the extrachromosomal target nucleic acid from the isolated micronuclei;hybridizing the nucleic acid from the isolated micronuclei with chromosomal DNA from the cell, wherein the isolated micronuclei nucleic acid is a probe; andidentifying the amplified target nucleic acid.