Patent ID: 8945918
Filing Date: 2015-02-03
Classification: C07K,C12N

Abstract:
1. A nucleic acid cassette for expressing in an insect cell a plurality of polypeptides encoded by a parvovirus capsid gene, the cassette comprising in 5′ to 3′ order: (i) a first insect cell operable promoter linked operably to a 5′ portion of a first ORF of the parvovirus capsid gene, the first ORF comprising a translation initiation codon, (ii) an intron comprising a second insect cell operable promoter, the second promoter operably linked to a 5′ portion of an at least one additional ORF of a parvovirus capsid gene, wherein the at least one additional ORF comprises at least one additional translation initiation codon and overlaps with the 3′ portion of the first ORF.