Patent ID: 6068839
Filing Date: 2000-05-30
Classification: A61P,C12N,C12P,C12R

Abstract:
TKR1912-I, an antibiotic having the following physicochemical properties (1), (2), (3), (4) and (5), or a pharmacologically acceptable salt thereof;(1) a fast atom bombardment mass spectrum (FAB-MS) with a peak at m/z559 (M+H).sup.+ ;(2) a carbon number of 26 and a nitrogen number of 0;(3) an ultraviolet absorption spectrum in methanol with terminal absorptions as defined in FIG. 1;(4) an infrared absorption spectrum according to the KBr method with the dominant absorption wavenumbers of 3430 cm.sup.-1, 2920 cm.sup.-1, 2850 cm.sup.-1, 1740 cm.sup.-1 and 1190 cm.sup.-1 as defined in FIG. 2; and(5) soluble in methanol, chloroform and water and practically insoluble in hexane;and obtained by cultivating the Aureobasidium FERM BP-5368 or a spontaneous or artificial mutant of Aureobasidium FERM BP-5368, recovering and isolating the objective antibiotic from the resulting culture broth;wherein the cultivating is carried out at 10.degree. C. to 30.degree. C. in a nutrient medium at a pH of 2 to 9 containing at least one carbon source, at least one nitrogen source and at least one inorganic salt until sufficient antibiotic activity is imparted to said medium; andthe recovering and isolating is carried out by extracting with a non-hydrophilic organic solvent or absorbing an antibiotic on an absorbent and desorbing or eluting with a solvent, and after the recovering and isolating, purification by column chromatography or high performance liquid chromatography is carried out.