Patent ID: 8975017
Filing Date: 2015-03-10
Classification: C12Q,Y10T

Abstract:
1. A process for concentrating nucleic acid molecules on a surface of a sensor field of a biochip of a sample, for detection of the nucleic acid molecules, comprising the following steps: (a) providing magnetic beads with transfer molecules immobilized thereon, which are configured to hybridize to a first portion of the nucleic acid molecules thereby forming complexes that comprise the magnetic beads, the transfer molecules, and the nucleic acid molecules; providing the surface of the sensor field, which forms a part of a surface of the biochip, wherein: capture molecules are immobilized on the surface of the sensor field; the capture molecules are configured to hybridize to a second portion of the nucleic molecules; and nucleic acid sequences of the capture molecules and the transfer molecules are selected such that a melting temperature of hybrids between the nucleic acid molecules and the transfer molecules is lower than a melting temperature of hybrids between the nucleic acid molecules and the capture molecules; and providing a magnetic field generator; (b) incubating the magnetic beads with the nucleic acid molecules at an incubating temperature of temperature T (c) applying a magnetic field through the magnetic field generator such that the nucleic acid molecules bound to the magnetic beads are moved through the magnetic field to the surface of the sensor field; (d) increasing the incubating temperature from the temperature T (e) moving the magnetic beads away from the surface of the biochip by switching off deactivating the magnetic field; and (f) repeating steps (b) to (e).