Patent ID: 6248578
Filing Date: 2001-06-19
Classification: A61K,C07K,C12N

Abstract:
A method for preparing recombinant human parainfluenza virus comprising;a. providing a recombinant system which comprises:i. an HPIV clone comprising a nucleotide sequence encoding a positive sense, antigenome of human parainfluenza virus;ii. a support clone comprising a nucleotide sequence encoding a human parainfluenza virus L protein; andiii. a support clone comprising a nucleotide sequence encoding a human parainfluenza virus P protein, wherein the nucleotide sequence encoding the P protein and L protein may be on the same support clone or on separate support clones;b. introducing the recombinant system of step (a) into host cells;c. culturing the host cells of step (b) for a time sufficient to permit transfection of the host cells and formation of recombinant human parainfluenza virus; andd. recovering the recombinant human parainfluenza virus from the culture of transfected host cells.