Patent ID: 6245531
Filing Date: 2001-06-12
Classification: A61K,C07K

Abstract:
An isolated, recombinant polynucleotide segment encoding an insect ecdysone receptor,wherein the ecdysone receptor binds both to an ecdysone response element and to ecdysone; wherein the segment comprises:(1) a nucleotide sequence encoding a steroid hormone receptor zinc finger DNA binding domain that binds an ecdysone response element, wherein the DNA binding domain comprises a plurality of cysteine residues, wherein the nucleotide sequence encoding the DNA binding domain hybridizes under selective hybridization conditions to nucleotides 1858 to 2055 of the DNA binding domain of Drosophila EcR of Table 2; and(2) a nucleotide sequence encoding a hormone binding domain that binds ecdysone, wherein the nucleotide sequence encoding the hormone binding domain hybridizes under selective hybridization conditions to nucleotides 2359 to 3021 of the ecdysone binding domain of Drosophila EcR of Table 2;wherein the selective hybridization conditions comprise a hybridization step in less than 500 mM salt and at least 37.degree. C., and washing in 2.times.SSPE at 63.degree. C.