Patent ID: 8318434
Filing Date: 2012-11-27
Classification: C12Q

Abstract:
1. A method for introducing a sample specific DNA tag into a plurality of DNA fragments from a plurality of samples comprising genetic material, said method comprising, for each of the samples, the steps of: (i) amplifying DNA fragments in a first multiplex or multiplex-like PCR reaction using amplicon specific forward and reverse primers, wherein each forward amplicon specific primer comprises a first adaptor sequence and wherein each reverse amplicon specific primer comprises a second adaptor, wherein the first adaptor sequence is identical in all forward primers for each of the samples and wherein the second adaptor sequence is identical in all reverse primers for each of the samples, (ii) further amplifying the amplified nucleic acids obtained in step (i), using one set of forward and reverse sample specific primers which are directed against said first and second adaptor sequences, wherein one or both of said sample specific primers comprises a DNA sequence which differs for each of the samples.