Patent ID: 9212382
Filing Date: 2015-12-15
Classification: C12Q,G01N

Abstract:
1. A method of screening a target agent for segment-specific immunostimulatory activity in a human epithelial cell, said method comprising: providing a cell system comprising an intestinal primary epithelial cell system for detecting gastrointestinal segment-specific activation or suppression of a Toll-like receptor (TLR) by a target agent, said system comprising: an isolated human intestinal primary epithelial cell (HIPEC) line that expresses in a segment-specific manner a plurality of TLRs selected from the group consisting of TLR1, TLR2, TLR3, TLR4, TLR5, TLR6, TLR7, TLR8, TLR9, TLR10, TLR11, TLR12, and TLR13, wherein said HIPEC line is derived from a differentiable adult human gastrointestinal stem cell (ahGISC) line derived from at least one human gastrointestinal segment selected from the group consisting of an esophagus segment, a stomach segment, a jejunum segment, an ileum segment, a duodenum segment, a cecum segment, an appendix segment, an ascending colon segment, a transverse colon segment, a descending colon segment, a sigmoid colon segment, and a rectum segment; contacting the cell system with a target agent; and subjecting the cell system to an immunostimultory assay to determine the immunostimulatory activity of the target agent on human epithelial cells.