Patent ID: 6589786
Filing Date: 2003-07-08
Classification: C12M,C12N

Abstract:
A suspension of cells obtained by practicing a method comprising the steps of:a. providing a biological sample comprising a cell suspension having a given cell population of at least a first and a second cell type, at least said first cell type being nucleated, wherein said first and second cell types have a difference in at least one property affecting a characteristic electroporation threshold; b. increasing the proportion of viable cells in the cell suspension that are viable cells of said second cell type without exposing the cell population to exogenous toxins by: i. subjecting the sample to electric field conditions sufficient to porate a substantial fraction of cells of said first cell type while maintaining substantially viable cells of said second cell type; and ii. selectively inactivating at least 90% of the porated cells of said first cell type in the sample subjected to the electric field conditions in step (b)(i), while maintaining substantially viable cells of said second cell type in the sample subjected to the electric field conditions in step (b)(i).