Patent ID: 8758768
Filing Date: 2014-06-24
Classification: A61K,A61P,C12P,Y02A

Abstract:
1. A method for producing one or more water-soluble extracellular immune stimulating agents, said method comprising the steps of: i) cultivating a fungus of the genus ii) separating the fungal mycelium from said aqueous liquid growth medium by centrifugation or filtration to obtain an aqueous phase supernatant comprising said one or more immune stimulating agents, and iii) subjecting said aqueous phase supernatant, or a fraction thereof, comprising said one or more water-soluble immune stimulating agents to at least one size fractionation step resulting in the isolation of an aqueous phase isolate which comprises one or more immune stimulating agents comprising a polysaccharide having a molecular weight in the size range of from 100,000 g/mol to 1,000,000 g/mol; wherein said aqueous phase supernatant comprising said one or more immune stimulating agents is not subjected to alcohol precipitation, and wherein said isolation of the aqueous phase isolate does not degrade or convert said immune stimulating agents during the isolation.