Patent ID: 6448093
Filing Date: 2002-09-10
Classification: A61K,A61P

Abstract:
A method for manufacture of a hydrophobic biomolecular structure consisting of hyaluronic acid and a polar substance, said hyaluronic acid surrounding the polar substance and said polar substance having a reduced size, wherein the method comprises:solubilization of the hyaluronic acid in an aqueous solution of an electrolyte and an acid, to form a mixture of solubilized hyaluronic acid, an electrolyte and an acid; solubilization of the polar substance in said mixture; adjusting the pH of said mixture including the solubilized polar substance to a pH below pH 3 thereby to expose hydrophobic domains of the polar substance; and dialyzing said mixture so as to reduce an ionic concentration thereof past the point of collapse for said polar substance, thereby to form a collapsed polar structure wherein said hyaluronic acid surrounds said polar substance; wherein said hyaluronic acid exposes at least part of its hydrophobic domains and surrounds, binds and stabilizes the polar substance by hydrophobic interactions, to form said hydrophobic biomolecular structure.