Patent ID: 7670810
Filing Date: 2010-03-02
Classification: C12Q

Abstract:
1. A method for detecting at least 10,000 typable loci comprising single-nucleotide polymorphisms (SNPs) of genome fragments, comprising: (a) providing a plurality of genome fragments of a whole genome, (b) contacting said plurality of genome fragments with an array of at least 10,000 predetermined nucleic acid probes immobilized to a substrate, (c) modifying the probe of a probe-fragment hybrid while hybridized to said genome fragments by adding a detectable nucleotide or nucleotide analog by a polymerase; (d) exposing said modified probes to denaturing conditions, thereby removing hybridized genome fragments; and (e) detecting said modified probes, thereby detecting at least 10,000 typable loci comprising SNPs.