Patent ID: 6893875
Filing Date: 2005-05-17
Classification: C12Q,G01N,Y10S,Y10T

Abstract:
1. A method for assay of an analyte which is an amplified gene product which comprises: (1) providing in a test solution a first probe that specifically hybridizes to said analyte, wherein said first probe is labeled with a chemiluminescent substance and said analyte comprises an amplified gene product; (2) adding to said test solution after said first probe has bound to said analyte, an unlabeled, second probe that competes with said first probe for specifically hybridizing to said analyte, wherein the ratio of unlabeled probe to labeled probe is 0.1-10 (3) quantitatively measuring a chemiluminescent signal, wherein said second unlabeled probe competes with said first probe for said analyte and results in signal quenching by physical reduction in the number of molecules of the first probe bound to the analyte; and (4) correlating said chemiluminescence to the presence of said analyte.