Patent ID: 7767409
Filing Date: 2010-08-03
Classification: G01N

Abstract:
1. A detection method comprising: exposing, to a surface to which a cofactor has been bound, said surface being a surface of a well of a microwell plate, and said binding between the surface and the cofactor being maintained by avidin-biotin binding between avidin or streptavidin immobilized on the surface and biotin bound to the cofactor, a nuclear receptor protein serving as a counterpart of the cofactor and a test sample; a tag protein being bound to said nuclear receptor protein; detecting a change in degree of binding between the nuclear receptor protein and the cofactor as a change in intensity as measured on the label of a labeled antibody which can bind to the tag protein; and detecting a substance which is contained in the test sample and which binds to the nuclear receptor, on the basis of the change in intensity as measured on the label of a labeled antibody which can bind to the tag protein, wherein the method employs two or more nuclear receptor proteins and cofactors serving as counterparts of the respective proteins, and the method is performed in different respective wells of a single microwell plate to thereby collectively detect substances which are contained in a test sample and which bind to the two or more nuclear receptors.