Patent ID: 6897036
Filing Date: 2005-05-24
Classification: C12Q,G01N,Y10S

Abstract:
1. A method for producing fluorescence comprising a) providing a peroxidase and a fluorogenic substrate having the formula wherein R 1 is a group selected to be inert to attack by peroxide and containing from 1 to 20 atoms selected from C, H, N, O, S, P, Si and halogens, R 2 is selected from alkyl, substituted alkyl, aryl substituted aryl, aralkyl and substituted aralkyl groups, and R 3 to R 10 are each groups containing from 1 to 20 atoms selected from C, H, N, O, S, P, Si and halogens; b) reacting the peroxidase with the fluorogenic substrate to produce a fluorescent product which accumulates in the reaction and undergoes no further reaction; and c) irradiating the fluorescent product at a suitable wavelength of light to cause the fluorescent product to emit fluorescence.