Patent ID: 8858927
Filing Date: 2014-10-14
Classification: A61K,C07K

Abstract:
1. A method of protecting the primary structure of peptides or proteins which contain one or more asparaginyl or glutaminyl residues with at least one deamidation prone sequence of -Asn-Gly- against non-enzymatic deamidation at at least said deamidation prone sequence, where the peptides or proteins are present in aqueous formulations or lyophylizates, comprising the step of combining said peptides or proteins with one or more stabilizing anions to form a mixture wherein said stabilizing anions are present in a molar excess to the total number of asparaginyl or glutaminyl residues in said peptides or proteins and are in a molar excess to any destabilizing anions in said mixture, wherein said stabilizing anions are organic compounds that are not zwitterionic where each protic group of said stabilizing anion has a pKa ranging from 0.5 to 3.5, wherein destabilizing anions have at least one protic group that has a pKa of less than 0.5 or greater than 3.5, and wherein said stabilizing anions are selected from the group consisting of saccharin, benzenesulfonic acid, gentisic acid, and N-acetyltryptophan.