Patent ID: 8729243
Filing Date: 2014-05-20
Classification: C07D,G01N

Abstract:
1. Method for preparing a compound having the formula (I): wherein X is biotin or Y is biotin or Z is biotin or V is biotin or B is NH AA is a trifunctional molecule derivative when B is NH AA G n T is an antiligand or a reactive group for fixation to an antiligand, said antiligand being capable of reacting with a ligand, the method comprising: (i) grafting n where R is a prefunctionalised resin, W and W to obtain a compound having the formula (III): (ii) coupling a compound having a formula (IV): where W (iii) grafting 2n (iv) when X in the formula (I) is biotin, passing directly to step (v); if not, when X in the formula (I) is not biotin, repeating the steps (ii) and (iii) with 2 and 2 (v) deprotecting the compound thus obtained at the W″ (vi) deprotecting, at the W group, the compound thus polybiotinylated and coupling with an antiligand or a reactive group for fixation to an antiligand (T) and (vii) cutting the compound thus obtained from the resin (R) to obtain a compound having the formula (I).