Patent ID: 7090999
Filing Date: 2006-08-15
Classification: C07K,C12N,C12P,C12R

Abstract:
1. A method for preparing pristinamycin IIA or IIB comprising: a) specifically introducing a disabling mutation into at least one gene target in a microorganism that produces pristinamycin IA and pristinamycin IB, wherein said gene target is selected from the snbA gene of SEQ ID NO: 5 and genes that hybridize to the snbA gene of SEQ ID NO: 5 of (b) selecting cells containing the mutated gene, wherein the cells are blocked in the production of pristinamycin IA and IB; (c) culturing said cells; and (d) recovering said pristinamycin IIA, pristinamycin IIB, or pristinamycin IIA and IIB.