Patent ID: 8367382
Filing Date: 2013-02-05
Classification: C12N,C12P

Abstract:
1. A method for producing 4-hydroxyisoleucine or a salt thereof comprising: A) subjecting isoleucine or a salt thereof to a hydroxylation reaction in the presence of a hydroxylase derived from a microorganism to produce a reaction product, and B) isolating 4-hydroxyisoleucine or a salt thereof from the reaction product, wherein the microorganism is wherein the hydroxylase comprises: (a) the ability to catalyze the production of (2S,3R,4S)-4-hydroxy-L-isoleucine by hydroxylation of L-isoleucine, wherein Fe (b) a molecular weight as measured by SDS-PAGE of about 29±2.0 kDa.