Patent ID: 6255059
Filing Date: 2001-07-03
Classification: C07K,C12N

Abstract:
A method for identifying a ligand for an orphan G protein coupled receptor (GPCR) comprising:(i) providing a mixture of recombinant yeast cells, each cell of which comprises(a) a first recombinant gene encoding a heterologous orphan GPCR protein, wherein said receptor is expressed on the cell membrane of said cell such that signal transduction activity via said receptor is modulated by interaction with an extracellular signal; and(b) a second recombinant gene encoding a test polypeptide, wherein said second recombinant gene is expressed and the test polypeptide is transported to a location allowing interaction with the receptor expressed on the cell membrane, andwherein collectively the mixture of cells expresses a library of said test polypeptides, said library capable of being expressed at a sufficient level such that modulation of the signal transduction activity of the receptor protein by a test polypeptide within the library provides a detectable signal;(ii) allowing cells to generate a detectable signal; and(iii) identifying the test polypeptide as a ligand for an orphan GPCR.