Patent ID: 7067252
Filing Date: 2006-06-27
Classification: C12N

Abstract:
1. A method for screening for compounds secreted by a microorganism, comprising: (a) cultivating the microorganism to produce a supernatant or a supernatant including surface bound compounds of the microorganism which comprises at least 2 secreted products, (b) preparing a mixture of antibodies by immunizing an animal with the supernatant comprising at least 2 secreted products and isolating the mixture of antibodies formed in the animal, (c) providing a gene library from the microorganism, (d) cloning the gene library into a suitable host organism, (e) screening clones of the host organism using isolated antibody mixture to identify positive clones expressing a cloned gene encoding a secreted compound, and (f) screening positive clones for peptides or proteins having a desired function or subjecting positive clones to nucleotide sequencing in order to identify genes encoding compounds of interest.