Patent ID: 6610479
Filing Date: 2003-08-26
Classification: C07K,C12N,C12Q,Y10S

Abstract:
A reversibly inactivated enzyme or a fragment thereof having catalytic activity of the enzyme, wherein the enzyme or fragment is in the form of a fusion protein that is attached to an immobilizing moiety via a pair of affinity binding partners, wherein the enzyme or fragment is essentially inactive when the fusion protein is attached to the immobilizing moiety and is activated by release of the fusion protein from the immobilizing moiety, wherein the enzyme is selected from the group consisting of DNA polymerases, DNA ligases, reverse transcriptases, and RNA polymerases, and wherein one partner of the pair of affinity binding partners is attached to or is inherently part of the immobilizing moiety, and the other partner of the pair of affinity binding partners is attached to or is inherently part of the fusion protein.