Patent ID: 7037688
Filing Date: 2006-05-02
Classification: C12Q

Abstract:
1. A method for amplifying and detecting a target nucleic acid in a test sample comprising the steps of: (a) forming an amplification mixture comprising a test sample, a first primer sequence and a second primer sequence, and amplifying agents, (b) amplifying the target sequence to generate copies of the target sequence comprising an amplification product from the first primer sequence and the second primer sequence, and (c) detecting the copies of the target sequence as an indication of the presence of the target nucleic acid sequence in the test sample, wherein the first primer sequence is present at a concentration between 100 nM and 250 nM, and the second primer sequence is present at a concentration of between 50 nM and 500 nM, and wherein the concentration of the first primer sequence and the second primer sequence differ by at least 15% and differ by no more than 250%, and wherein the presence of the target nucleic acid is indicated by the presence of an amplification product and amplification and detection are efficiently increased by avoiding plateauing of signals.