Patent ID: 6331434
Filing Date: 2001-12-18
Classification: C08G,C12N

Abstract:
Method of transforming cells in which an appropriate quantity of nucleic acid fragments is introduced into the cells, characterized in that the nucleic acid fragments are introduced in the form of a nucleic acid composition which comprises, in an appropriate vehicle, at least one nucleic acid fragment and at least one compacting agent comprising a nitrogen-containing silicone, wherein the nitrogen-containing silicone comprises at least one silicone unit which follows:Si R1 O.sub.(3-x-y)/2 R2.sub.x R3.sub.yin which:R1 is a nitrogen-containing hydrocarbon radical,R2 represents the R1 radical, a hydroxyl radical or a hydrocarbon radical,R3 represents R2 or the radical of formula ##STR5##in which R2 is defined above,x and y, which are identical or different are 0, 1 or 2, it being understood that the sum x+y is less than or equal to 3.