Patent ID: 6531278
Filing Date: 2003-03-11
Classification: C07H,C07K,G01N,Y10S

Abstract:
A method for capturing a target on a solid support comprising:(a) mixing an aqueous sample containing the target with a first composition comprising a first ligand component, configured for binding the target, covalently bonded to a first nucleic acid component such that the first ligand component binds the target to result in a complex; (b) contacting the mixed sample with a second composition comprising (i) a solid support covalently bonded to a linker, (ii) a second nucleic acid component covalently bonded to the linker wherein the second nucleic acid component is complementary to at least a portion of the first nucleic acid component and hybridizes thereto when in contact therewith, resulting in a duplex that has a thermal melting temperature of about 60-85Â° C., and (iii) a second ligand component covalently bonded to the second nucleic acid component, such that the second nucleic acid component hybridizes to the first nucleic acid component and the second ligand component binds the target; (c) heating the contacted sample to a temperature above the thermal melting temperature of the duplex without denaturing the first ligand component or second ligand component and causing the heated aqueous sample to flow by the solid support, thereby washing away non-target bound first ligand-first nucleic acid component conjugates; and (d) then reducing the temperature of the heated sample to ambient temperature, thereby capturing the target on the solid support.