Patent ID: 6623966
Filing Date: 2003-09-23
Classification: C12N

Abstract:
A method of increasing an expression of a wild-type nucleic acid and inhibiting a transcription of a mutant nucleic acid having a mutation in a eukaryotic cell, comprising:(a) transfecting the cell with a first composition including a first PNA amino acid chimera having a nuclear localization sequence and a first segment of peptide nucleic acid residues that are complementary to a first binding site on a non-coding strand of the wild-type nucleic acid; and (b) transfecting the cell with a second composition including a second PNA amino acid chimera having the nuclear localization sequence and a second segment of peptide nucleic acid residues that are complementary to a second binding site on a coding strand of the mutant nucleic acid and wherein the second binding site includes the mutation; and (c) allowing the first composition to anneal to the non-coding strand of the wild-type nucleic acid and the second composition to anneal to the coding strand of the mutant nucleic acid having the mutation, thereby increasing the expression of the wild-type nucleic acid and inhibiting the transcription of the mutant nucleic acid.