Patent ID: 6084080
Filing Date: 2000-07-04
Classification: A61K

Abstract:
An extracting process for producing a breviscapinum which has a chemical structure of 4',5,6-trishydroxyflavone-7-glucuronide, comprising the steps of:(a) providing an erigeron breviscapus powder by grinding whole plant of erigeron breviscapus;(b) soaking said erigeron breviscapus powder with industry ethanol to form a soaking solution;(c) concentrating said soaking solution until a cream color volatile oil is distilled out of said soaking solution to form a concentrate;(d) dissolving said concentrate in boiling water to obtain a water dissolvable filtered solution;(e) degreasing said filtered solution to form a degreased filtered solution;(f) extracting said degreased filtered solution with chloroform to form a first extracted and a chloroform liquid;(g) extracting said first extracted solution with ethyl ether to form a second extracted solution and an ethyl ether liquid;(h) extracting said second extracted solution with ethyl acetate to form a third extracted solution and an ethyl acetate liquid;(i) extracting said third extracted solution with lead acetate to form a fourth extracted water dissolvable and an extracted precipitate in yellow color;(j) filtering out said extracted precipitate;(k) suspending said extracted precipitate in ethanol and add diluted sulphuric acid to filter and separate out lead content in order to obtain an ethanol solution and a lead sulphuric acid;(l) filtering, depressurizing and concentrating said ethanol solution to an adequate volume until yellow precipitate is obtained, wherein said yellow precipitate is in tiny needle crystalline when observing under microscope;(m) further washing and filtering said yellow precipitate with ethanol to obtain a final precipitate; and(n) drying said final precipitate to obtain a coarse breviscapinum.