Patent ID: 7736874
Filing Date: 2010-06-15
Classification: A61K,C07K,C12N

Abstract:
1. A method for cloning one or more nucleic acid molecules having a first 3′ overhang into one or more vectors having a second 3′ overhang, comprising: (a) forming a mixture comprising the one or more nucleic acid molecules having the first 3′ overhang, the one or more vectors having the second 3′ overhang and one or more antibodies or fragments thereof which inhibit a polymerase; and (b) ligating the nucleic acid molecules having the first 3′ overhang to the one or more vectors having the second 3′ overhang to form one or more genetic constructs, thereby cloning the one or more nucleic acid molecules having the first 3′ overhang.