Patent ID: 7655419
Filing Date: 2010-02-02
Classification: A61P,C07K,C12N,C12Q,G01N

Abstract:
1. A method of screening a test agent for activity in modulating the replication of a Flaviviridae virus, the method comprising: contacting a test agent with a NS5A polypeptide, a TBC1D20 polypeptide, and, optionally, a Rab1 polypeptide and/or an Arf1 polypeptide, said contacting being under conditions suitable for TBC1D20 to activate a GTPase; and detecting the presence or absence of a change in an activity of at least one of the TBC1D2 polypeptide, the Rab1 polypeptide, and the Arf1 polypeptide; wherein the presence in a change in activity of at least one of the TBC1D20 polypeptide, the Rab1 polypeptide, and the Arf1 polypeptide indicates the test agent has activity as an antiviral agent.