Patent ID: 6287863
Filing Date: 2001-09-11
Classification: A61K,C12N

Abstract:
A method of transferring a DNA sequence to an oviduct or embryo of an egg laying species, comprising:(a) introducing a DNA transfer vector into a cell in vitro to yield a transformed cell which produces recombinant virus, wherein the recombinant virus comprises RNA transcribed from the DNA vector, and wherein the DNA transfer vector comprises linked:(i) a 5' long terminal repeat (LTR) sequence derived from a retrotransposon comprising a transcription initiation site for RNA;(ii) an encapsidation sequence positioned 3' of the 5' LTR;(iii) a primer binding site sequence positioned 3' of the 5' LTR;(iv) a 3' LTR sequence derived from a retrotransposon positioned 3' of the primer binding site which includes:(1) sequences necessary for polyadenylation of a RNA transcript initiated in the 5' LTR;(2) sequences necessary for reverse transcription of the RNA transcript into a double stranded cDNA;(v) a polypurine tract sequence located 5' to the 3' LTR;(vi) a DNA sequence comprising at least one open reading frame inserted into the vector 3' of the transcription initiation site in the 5' LTR of the vector; and(vi) sequences within each LTR which are necessary for integration of the biologically active transfer vector into the genome of a recipient cell, wherein the vector sequences of (i), (ii), (iii), (iv), and (v) comprise no more than 2 kbp;(b) introducing the recombinant virus to the oviduct or embryo of the egg laying species; and(c) identifying egg laying animals which express the DNA sequence.