Patent ID: 9181528
Filing Date: 2015-11-10
Classification: C12N

Abstract:
1. A method to derive a population of pancreatic endocrine precursor cells from pluripotent stem cells comprising the steps of: a. Culturing a population of pluripotent stem cells; b. Differentiating the population of pluripotent stem cells into a population of cells expressing markers characteristic of the definitive endoderm lineage; c. Differentiating the population of cells expressing markers characteristic of the definitive endoderm lineage into a population of primitive gut tube cells; d. Differentiating the population of primitive gut tube cells into a population of posterior foregut cells; and e. Treating the population of posterior foregut cells with a medium supplemented with a CYP26A inhibitor and with no added retinoic acid such that the population of posterior foregut cells differentiates into a population of pancreatic endocrine precursor cells, wherein expression of endocrine precursor markers is increased and wherein the differentiating in steps b., c. and d. comprises treatment in a medium lacking a CPY26A inhibitor.