Patent ID: 6503702
Filing Date: 2003-01-07
Classification: G01N,Y10S,Y10T

Abstract:
A kit for detection by immunoassay of at least one test substance in a test sample comprising in packaged combination:(a) a primary antibody which immunologically recognizes said test substance wherein said primary antibody is conjugated to a means of detection; (b) a cylindrical solid phase format contained in a cylindrical vessel, the vessel having an inner wall and at least one open end, said solid phase format being circumferentially and tightly pressed against the inner wall of said vessel, having a pore size of about 20-30 micrometers with a plurality of interstitial spaces and capable of capturing a complex formed by said primary antibody and said test substance; (c) a second vessel containing an ExLISA buffer system which is capable of supporting simultaneous sample extraction of said test substance from said test sample and antigen-antibody reaction consisting essentially of: (i) a buffer; (ii) a non-ionic detergent present at a concentration of about 0.01 to about 0.2% (w/v); (iii) a salt in an amount effective to provide a physiological saline solution and present at a concentration of about 25 mM to about 175 mM; (iv) a chelator in an amount effective to remove multivalent ions; (v) stabilizing agent in an amount effective to maintain stability and activity of an antibody in said antigen-antibody reaction and present at a concentration from about 0.01 to about 20% (w/v); (vi) a phenolic compound inhibitor; (vii) a protease inhibitor; (viii) a protein in an amount effective to immunologically block non-specific antibody binding and present at a concentration from about 0.05 to 5% (w/v); (d) reagents reactive with said means of detection to produce a detectable reaction product; and (e) means for dispensing said reagents.