Patent ID: 6448017
Filing Date: 2002-09-10
Classification: C12N,C12Q

Abstract:
An in vitro composition for exponentially amplifying in vitro a target polynucleotide region of a nucleic acid molecule, wherein said composition comprises:(A) a single-stranded first polynucleotide, wherein said polynucleotide (i) contains a polynucleotide region that is complementary in sequence to said target polynucleotide region, and (ii) is a circular polynucleotide or is circularizable when hybridized to a polynucleotide comprising said target polynucleotide region; (B) a second polynucleotide said second polynucleotide being a primer having a 3â€² terminus hybridized to said single-stranded first polynucleotide and comprising said target polynucleotide region; (C) a third polynucleotide, said third polynucleotide being a primer and comprising a polynucleotide region that is complementary in sequence to a region of said second polynucleotide, and whose 3â€² terminus is hybridized thereto; and (D) a polymerase, wherein said polymerase extends the 3â€² termini of said second and third polynucleotides in a template-dependent manner to thereby provide said exponential amplification of said target polynucleotide region.