Patent ID: 6365418
Filing Date: 2002-04-02
Classification: B01J,B82Y,C07K,C40B,G01N

Abstract:
An array device comprisinga substrate having a surface; and, one or more protein immobilization regions on said surface, said protein immobilization regions each comprising; (i) an ordered hydrophobic monolayer formed of alkyl chains having proximal ends which are chemisorbed or physisorbed to said surface within said immobilization regions, and opposite hydrophobic distal ends, (ii) a hydrophilic monolayer attached to said ordered hydrophobic monolayer, said hydrophilic monolayer comprising hydrophilic chains, each hydrophilic chain having a proximal end by which said hydrophilic chain is linked to an alkyl chain distal end, and an opposite hydrophilic distal end, and, (iii) a plurality of functional groups attached to said hydrophilic/hydrophobic monolayers for covalently attaching protein-capture agents to said hydrophilic/hydrophobic monolayers within said immobilization regions; wherein said hydrophobic monolayer and hydrophilic monolayers together are effective to resist non-specific protein binding.