Patent ID: 6750030
Filing Date: 2004-06-15
Classification: G01N,Y10S

Abstract:
A method of detecting cardiac ischemia in a mammal, comprising the steps of:providing a serum or plasma sample obtained from said mammal; mixing said serum or plasma sample with an aqueous solution and with a reagent comprising a fatty acid binding protein labeled with a fluorescent moiety, wherein said reagent exhibits a first fluorescence in an aqueous solution and a measurably different second fluorescence in an aqueous solution when said fatty acid binding protein is bound to a free fatty acid, wherein said free fatty acid is unbound to serum albumin; measuring said second fluorescence after said serum or plasma sample is mixed with said aqueous solution and said reagent to determine a concentration of said free fatty acid in said serum or plasma sample; and determining whether said concentration of said free fatty acid in said serum or plasma sample is indicative of cardiac ischemia by determining that said concentration of said free fatty acid unbound to serum albumin in said serum or plasma sample is at least about two standard deviations greater than an average concentration of free fatty acid unbound to serum albumin in serum or plasma of a control population that does not have cardiac ischemia.