Patent ID: 7348408
Filing Date: 2008-03-25
Classification: C07K

Abstract:
1. An isolated recombinant fusion polypeptide having an N-terminus and a C-terminus, wherein the fusion polypeptide comprises: (a) a fusion protein partner located towards the N-terminus of the fusion polypeptide, in which the fusion protein partner consists of a TolAIII domain defined by the amino acid sequence of SEQ ID NO: 13; and (b) a non-TolA polypeptide located towards the C-terminus of the fusion polypeptide, in which the non-TolA polypeptide is other than an His tag, wherein the fusion protein partner functions to facilitate higher levels of expression of the non-TolA polypeptide in an isolated host cell compared with expression in the isolated host cell of the non-TolA polypeptide lacking the fusion protein partner.