Patent ID: 7355038
Filing Date: 2008-04-08
Classification: C07H,C12N

Abstract:
1. A phase isolation process for biomacromolecule components, comprising treating a sample comprising a substance to be isolated, wherein said substance is plasmid DNA, said process comprising: 1) Preparing a buffer by dissolving 380 g -480 g ammonium sulfate or cesium chloride, and 14 g -18 g monosodium orthophosphate in water, and bringing the volume to 1 liter; 2) Adding a sufficient amount of the buffer from step (1) to said sample and homogenizing, so as to carry out agglutination; 3) Adding a phase isolation solution comprising a mixture of ethanol and isopropyl alcohol and the ratio of volume is ethanol:isopropyl alcohol=2:1-10:1, homogenizing, and centrifuging to form an upper phase, a lower phase, phase middle precipitation, and lower phase precipitation; wherein lipid is in the upper phase, protein precipitates in the phase middle, DNA plasmid, viral nucleic acid, mitochondrial DNA are in the lower phase, and total RNA precipitates in the lower phase; and genomic DNA precipitates in the lower phase or in the phase middle together with protein.