Patent ID: 6821761
Filing Date: 2004-11-23
Classification: C07F,C07H,C12N,C12P

Abstract:
A method for producing a lysosphingolipid which comprises:treating a sphingolipid with a sphingolipid ceramnide N-deacylase obtainable from a strain belonging to a species of the genus Pseudomonas and having physicochemical properties of: (1) acting on a ceramide moiety in the molecule of a sphingolipid and forming a lysosphingolipid and a fatty acid; (2) acting on neutral glycosphingolipids, acidic glycosphingolipids, and sphingomyelins; (3) having a molecular weight of about 52 kDa as determined by SDS-PAGE; and (4) having an optimum temperature of about 40Â° C., to obtain a reaction mixture; and recovering a lysosphingolipid from the reaction mixture.