Patent ID: 7358059
Filing Date: 2008-04-15
Classification: G01N,Y10S,Y10T

Abstract:
1. A method for the simultaneous quantification of the levels of expression of the CD55 and CD59 phosphatidyl-inositol glycan complementation class A (PIG-A) anchored cell membranes proteins in red cells, platelets, lymphocytes, monocytes, neutrophils and eosinophils present in a blood sample aliquot, comprising the steps of: incubating the blood sample with a mixture of fluorochrome-conjugated monoclonal antibodies directed against the CD45, CD61, CD55 and CD59 cell surface proteins; measuring the intensity of the fluorescence emissions of the fluorochrome-conjugated monoclonal antibodies bound to the cells contained in said incubated blood sample; measuring the light scattering properties of the bound cells contained in said incubated blood sample; identifying the different subsets of red cells, platelets, lymphocytes, monocytes, neutrophils and eosinophils present in said blood sample based on their differential expression of CD45 and CD61 and different quantities of both forward (FSC) and sideward (SSC) light scattering properties, and; simultaneously quantifying the expression of CD55 and CD59 for each of said blood cell subsets, wherein the CD45 and CD61 monoclonal antibodies are conjugated to the same fluorochrome and the CD55 and CD59 monoclonal antibodies are conjugated to a second and a third different fluorochromes.