Patent ID: 8722402
Filing Date: 2014-05-13
Classification: C12N,G01N

Abstract:
1. A method for assaying immunogenicity of a test agent comprising: a) loading a vaccination site comprising a first matrix and a plurality of cells attached to said matrix with blood monocytes and non-monocytic dendritic cell precursors; b) contacting the blood monocytes and non-monocytic dendritic cell precursors in the culture of a) with a test agent; c) culturing the culture of b) under conditions promoting conversion of the blood monocytes and non-monocytic dendritic cell precursors into mature antigen-presenting cells; d) transferring the mature antigen-presenting cells of the culture of c) to a three-dimensional artificial lymphoid tissue comprising a second matrix and a plurality of lymphocytes; e) culturing the culture of d) under conditions promoting stimulation of the lymphocytes by the mature antigen-presenting cells; and f) evaluating a response by the lymphocytes to stimulation by the mature antigen-presenting cells, wherein said response correlates with the immunogenicity of the test agent.