Patent ID: 9206481
Filing Date: 2015-12-08
Classification: A61P,C12N,C12Q

Abstract:
1. A method of detecting the expression of Ets Related Gene 8 (ERG8) mRNA in a biological sample comprising nucleic acid sample isolated from human prostate epithelial cells, the method comprising: (a) combining the nucleic acid sample isolated from human prostate epithelial cells with at least a first and a second oligonucleotide primer under hybridizing conditions, wherein the first and the second oligonucleotide primers amplify a target sequence comprising a 3′ non-coding region within nucleotides 1311 to 2441 of SEQ ID NO: 30, and wherein the entire nucleotide sequence of at least one of the first or the second oligonucleotide primers hybridizes to a region within said nucleotides 1311 to 2441 of SEQ ID NO:30 or a nucleic acid strand complementary to said region; (b) amplifying a plurality of amplification products when said nucleotides 1311 to 2441 of SEQ ID NO:30 are present in the nucleic acid sample by adding at least one polymerase activity to the biological sample containing the first and second oligonucleotide primers, wherein the amplification products comprises the region within nucleotides 1311 to 2441 of SEQ ID NO: 30 to which the entire nucleotide sequence of at least one of the first or the second oligonucleotide primers hybridizes; (c) immobilizing the plurality of amplification products; (d) combining an oligonucleotide probe with the immobilized plurality of amplification products to thereby permit the probe to hybridize to at least one immobilized amplification product; (e) detecting whether a signal results from hybridization between the oligonucleotide probe and at least one amplification product, and (f) detecting expression of ERG8 mRNA in the biological sample if the signal is detected in step (e).