Patent ID: 6277369
Filing Date: 2001-08-21
Classification: A61K,A61P,C12N,G01N

Abstract:
A method for providing a mammal with a therapeutic factor IX protein comprising:a) capturing TGF.beta.1-responsive, bone marrow-derived cells under low serum conditions in a collagen matrix impregnated with a TGF.beta.1 fusion protein comprising a von Willebrand's factor-derived collagen binding site which targets the TGF.beta.1 protein to the collagen matrix;b) expanding the captured cells to form differentiated cell colonies;c) isolating the expanded cells;d) transducing the expanded cells in vitro with a viral vector comprising a nucleic acid encoding a factor IX protein; ande) introducing the transduced cells into the mammal, wherein the transduced cells express the therapeutic factor IX protein in vivo.