Patent ID: 6146854
Filing Date: 2000-11-14
Classification: B01D,B01L,C12N

Abstract:
A process for isolating plasmid DNA from plasmid-containing cells, comprising the steps of:a) washing plasmid-containing cells by filtration with a wash solution to disrupt the cell membrane and degrade RNA;b) incubating the resulting washed plasmid-containing cells with a lysis/denaturation solution to lyse the cells and denature DNA;c) incubating the product of step b) with a renaturation solution for an appropriate period of time and at an appropriate temperature to yield a renatured mixture containing dissolved plasmid DNA, insoluble linear DNA and cellular debris;d) filtering the product of step c) to obtain a filtrate from which insoluble linear DNA and cellular debris have been removed; ande) obtaining plasmid DNA from the filtrate, wherein no centrifugation step is used in the process.