Patent ID: 8293477
Filing Date: 2012-10-23
Classification: A61P,C07K,C12N

Abstract:
1. A method of identifying compounds useful in modulating bone formation and mineralization comprising, a) providing an indicator composition comprising recombinant KRC; b) contacting the indicator composition with each member of a library of test compounds; c) selecting from the library of test compounds a compound of interest that modulates a biological activity of KRC, wherein the biological activity of KRC is selected from the group consisting of modulation of osteocalcin gene transcription, modulation of Runx2 degradation, modulation of Runx2 ubiquitination, modulation of RSK2 expression, modulation of RSK2 degradation, modulation of RSK2 ubiquitination, modulation of RSK2 phosphorylation, modulation of the expression of BSP, ColI(α)1, OCN, Osterix, RANKL, and/or ATF4, modulation of ATF4 protein levels, and/or modulation of ATF4 phosphorylation; d) testing the ability of the compound selected in step c) to modulate bone formation and mineralization; and e) selecting a compound that modulates bone formation or mineralization to thereby identify a compound useful in modulating bone formation and mineralization.