Patent ID: 8494780
Filing Date: 2013-07-23
Classification: C12Q,G01N,G06K,G16B

Abstract:
1. A method of developing an assay for a target, comprising: providing an assay, comprising: measuring one or more paired fluorescence intensities in each sample, wherein the fluorescence intensity pair consists of a fluorescence intensity of the negative control signature and a fluorescence intensity of the positive signature; determining, by linear regression, a correlation between the fluorescence intensities of the negative control signatures and the fluorescence intensities of the positive signatures; determining the distribution of orthogonal distances from the regression line to the points consisting of the paired fluorescence intensity measures; and choosing a threshold for the distance based on the distribution of orthogonal distances, wherein a distance between a measured fluorescence intensity pair and the regression line above the threshold the indicates a positive result in the assay, and a distance between a measured fluorescence intensity pair and the regression line below the threshold indicates a negative result in the assay.