Patent ID: 8993306
Filing Date: 2015-03-31
Classification: C12N

Abstract:
1. A method for separating genomic prokaryotic DNA of Mollicutes from chromosomal eukaryotic DNA from a biological sample, comprising the following steps in the following order: a) centrifugation of a eukaryotic cell suspension with a cell density of up to 10^10 cells/ml and re-suspending the cells in the presence of chelating agent EDTA, b) lysis of the cells by chemical lysis in the presence of a detergent SDS and base NaOH, c) neutralizing the cell lysate in the presence of at least one salt sodium potassium acetate, and d) separating precipitated eukaryotic DNA and obtaining the prokaryotic genomic DNA by silica column chromatography, wherein a detection threshold of <10 cfu/ml or <10 copies/ml is obtained by amplifying the genomic prokaryotic separated DNA from the eukaryotic cell suspension with a cell density of up to 10^10 cells/ml.