Patent ID: 7049103
Filing Date: 2006-05-23
Classification: C12Q,G01N

Abstract:
1. A method of assaying the efficacy andlor toxicity of a test substance by expression of a specific gene in a cell or a microorganism, which comprises: (a) treating the cell or the microorganism with the test substance, (b) extracting nucleic acids from the cell or the microorganism to provide a reaction solution, (c) amplifying, from said reaction solution containing nucleic acids, a target RNA having a sequence homologous or complementary to a specific sequence in a target mRNA in the reaction solution which is obtained by said extracting by a method comprising, (d) determining whether the target mRNA is transcribed through the expression of the specific gene by detecting the RNA amplified by said amplifying, and (e) assessing the efficacy and/or toxicity of said test substance by expression of the specific gene compared to expression of said the specific gene when in the absence of said test substance, wherein (c) and (d) are performed simultaneously.