Patent ID: 7211385
Filing Date: 2007-05-01
Classification: C12Q

Abstract:
1. A method of detecting cancer, a pre-cancerous condition or genetic disorder in a subject comprising amplifying a region of DNA from a subject, wherein said region comprises a microsatellite comprising a mononucleotide repeat, wherein said amplification comprises the steps of: (a) providing a sample comprising a nucleic acid that contains a nucleic acid having a microsatellite instability, (b) amplifying at least one nucleobase sequence of said nucleic acid, said nucleobase sequence comprising at least one of said microsatellites; and (c) detecting alterations of said microsatellite as compared to corresponding microsatellites amplified from control tissue; said amplified microsatellite having a G+C content of 50% or less; wherein said amplification is performed in the presence of a sufficient amount of an additive selected from the group consisting of sorbitol, betaine and mixtures thereof, wherein said additive is effective to reduce said stutter relative to the amount of stuffer observed in the absence of said additive, wherein the cancerous condition is colorectal cancer, and wherein the mononucleotide repeat is BAT-25, BAT-26, or BAT-25 and BAT-26.