Patent ID: 6613560
Filing Date: 2003-09-02
Classification: B01L,B29C,G01N

Abstract:
In a device for conducting the polymerase chain reaction (PCR) process comprising a reaction chamber defined by two or more interior surfaces, a means for introducing PCR reaction components into the chamber, a means for removing the PCR reaction product from the chamber, and a means for controlling the temperature of the reaction chamber, the improvement which comprises:fabricating the device from a chemically inert material that does not contain silicon and is thermally, chemically and mechanically stable under the conditions at which a PCR reaction is conducted, and employing a reaction chamber adapted to contain in the range of approximately 1 &mgr;l to 500 &mgr;l of fluid, wherein the interior surfaces of the reaction chamber are coated with a sorption-reducing polyethylene oxide layer so as to enhance thermal stability, chemical stability and biofouling resistance.