Patent ID: 6461809
Filing Date: 2002-10-08
Classification: G01N

Abstract:
A method of improving the HIV receptivity of a continuous cell line transformed with DNAs coding for expressible CD4 and expressible HIV-1 coreceptor in an assay for HIV-1, comprising:a) positively-selecting cells which co-express CD4 and HIV-1 coreceptor on their cell surface, thereby improving their HIV receptivity in comparison to cells not positively-selected, wherein said cells have been transformed with DNAs coding for expressible CD4 and expressible HIV-1 coreceptor; b) contacting said positively-selected cells with HIV-1 under conditions effective for said HIV-1 to infect said cells; and c) detecting the number of cells infected with HIV-1, thereby assaying for infectivity of HIV-1.