Patent ID: 6077835
Filing Date: 2000-06-20
Classification: A61K,C07H,C07K,C12N,C12Y

Abstract:
A method of achieving expression of a protein in a subject comprising:administering to a subject a composition comprising unaggregated nucleic acid complexes, each complex consisting essentially of a single nucleic acid molecule encoding a protein and one or more carrier molecules, said carrier molecule having a nucleic acid binding moiety through which it is complexed to the nucleic acid wherein said complex is compacted to a diameter which is less than (a) double the theoretical minimum diameter of a complex of said single nucleic acid molecule and a sufficient number of carrier molecules to provide a charge ratio of 1:1, in the form of a condensed sphere, or (b) 30 nm, whichever is larger, whereby said protein is expressed in the subject.