Patent ID: 6660517
Filing Date: 2003-12-09
Classification: B01D,B01F,B01J,B01L,C12M,C12Q,C40B,Y10S,Y10T

Abstract:
A device for conducting a polynucleotide amplification reaction, the device comprising:a sample introduction system for introducing a sample into said device, which comprises an inlet port and a vent; at least one sample flow channel having a cross-section and extending from said inlet port; a solid substrate fabricated to include at least one polynucleotide amplification reaction chamber in fluid communication with said flow channel and said vent, said reaction chamber being permanently sealed with a cover and having a cross-section, the cross-section of said reaction chamber having at least one mesoscale dimension of width or depth which is between about 0.1 to 1,000 &mgr;m, said reaction chamber cross-section and said flow channel cross-section being dissimilar; and a fluid delivery apparatus for delivering fluid to and receiving fluid from said inlet port, wherein said fluid delivery apparatus interfits with said inlet port, and reversibly seals said inlet port.