Patent ID: 6066480
Filing Date: 2000-05-23
Classification: C12P

Abstract:
A process for producing .alpha.,.omega.-alkanedicarboxylic acids, comprising:(a) culturing Candida tropicalis cells during a growth phase in a culture medium containing at least a nitrogen source and a carbon source, which is glucose present at a concentration of about 10 g/L to about 30 g/L, at a temperature of about 33.degree. C. to about 35.degree. C. wherein additional glucose is continuously added through the growth phase at a rate of about 1 g/L/h to about 4 g/L/h;(b) initiating a conversion phase by addition of a substrate selected from the group consisting of fatty acids, fatty acid esters and alkanes within 0-3 hours after the maximum growth of the cultured cells is attained, and(c) continuing culturing the cells during the conversion phase wherein glucose is added during the conversion phase at a rate of about 1 g/L to about 4 g/L.