Patent ID: 9163079
Filing Date: 2015-10-20
Classification: A61P,C07K,G01N

Abstract:
1. Monovalent fusion polypeptide which is present in the form of a monomer under non-denaturing conditions, comprising (i) a first domain comprising the heavy chain variable region of an antibody (VH) or at least a section thereof that mediates antigen binding, (ii) a second domain comprising the light chain variable region of an antibody (VL) or at least a section thereof that mediates antigen binding, and (iii) a third domain comprising a section of a heavy chain constant region of an antibody (CHX), wherein domain (iii) has a length of from 80 to 130 amino acid residues, wherein domains (i), (ii) and (iii) are linked together via peptide linkers (L), wherein the peptide linkers (L) each independently have a length of from 25 to 45 amino acid residues and consist of sequences which are heterologous to the amino acid sequences of the first, second and third domains, wherein the monovalent fusion polypeptide has antigen binding activity and over 90% of said antigen binding activity remains after 10 days incubation at 36° C. or after drying and reconstitution or after freeze/thaw cycles repeated 5 times and wherein the monovalent fusion polypeptide does not form intermolecular disulphide bridges and is free of hinge regions of antibodies.