Patent ID: 6531316
Filing Date: 2003-03-11
Classification: C12N

Abstract:
A method of unencrypting a trait encrypted gene sequence to provide at least one unencrypted RNA or polypeptide, the method comprising:providing a first parental organism and a second parental organism, wherein the first parental organism comprises a first split gene sequence comprising a first subsequence of a genetic element, the second parental organism comprises a second split gene sequence comprising a second subsequence of the genetic element, and wherein the second split gene sequence is not present in the first parental organism and the first split gene sequence is not present in the second parental organism; mating the first parental organism with the second parental organism to produce a progeny organism comprising the first split gene sequence and the second split gene sequence, wherein the split gene sequences are transcribed to provide a plurality of RNA segments; and trans-splicing at least two of the plurality of RNA segments together to provide at least one unencrypted RNA; or, alternately, translating the plurality of RNA segments to provide a plurality of polypeptide segments and trans-splicing at least two of the plurality of polypeptide segments together to provide at least one unencrypted polypeptide.