Patent ID: 7659149
Filing Date: 2010-02-09
Classification: G01N

Abstract:
1. A method of sensing a target nucleic acid in an electrolyte using a bio field effect transistor (FET), the method comprising: (a) forming a layer comprising Au on a gate of a bio field effect transistor (bioFET); (b) bonding a nucleic acid having a thiol group (—SH) to a probe nucleic acid immobilized on a substrate separated from the gate by a predetermined distance, wherein the nucleic acid having a thiol group is incompletely bonded to the probe nucleic acid; (c) contacting the probe nucleic acid with a sample including a target nucleic acid, wherein a bonding force between the probe nucleic acid and the target nucleic acid is greater than a bonding force between the probe nucleic acid and the nucleic acid having a thiol group; (d) separating the nucleic acid having a thiol group from the probe nucleic acid by bonding the target nucleic acid to the probe; (e) bonding the thiol group of the nucleic acid having a thiol group with Au on the gate and (f) measuring a current flowing in a channel region between a source and a drain of the bioFET.