Patent ID: 6242182
Filing Date: 2001-06-05
Classification: A01K,C12Q

Abstract:
A method for determining the haplotype of the Rfp-Y gene of a chicken, which comprises:(a) obtaining a sample of nucleic acid from a chicken which contains Rfp-Y genomic DNA, wherein said Rfp-Y genomic DNA is subject to allelic variation;(b) performing polymerase chain reaction (PCR) using a primer pair from the .alpha.1 domain of the Rfp-Y class I gene to amplify said Rfp-Y qenomic DNA and to produce amplification products;(c) denaturing said amplification products to produce denatured amplification products;(d) subjecting said denatured amplification products to non-denaturing electrophoretic separation to produce an electrophoresis pattern; and(e) comparing said electrophoresis pattern to at least one electrophoresis pattern produced by a standard nucleic acid sample obtained, amplified, denatured and subjected to electrophoretic separation according to steps (a)-(d), thereby determining the haplotype of the Rfp-Y gene of the chicken, wherein said standard nucleic acid is obtained from a chicken of known Rfp-Y Class I haplotype.