Patent ID: 6514705
Filing Date: 2003-02-04
Classification: C12Q

Abstract:
A method for determining lineage of scallop, comprising:extracting DNA from an adductor muscle of a scallop to serve as a template DNA; amplifying mitochondrial DNA from said template DNA by polymerase chain reaction, using a first primer set designed on the basis of nucleotides conserved in shellfish mitochondrial 16S rRNA genes and a second primer set designed on the basis of nucleotides conserved in shellfish mitochondrial 12S rRNA genes: sequencing the amplified mitochondrial DNA to obtain a nucleotide base sequence a said mitochondrial DNA; determining at least one non-coding region in said nucleotide base sequence; sequencing said at least one non-coding region, using a third primer set designed on the basis of said nucleotide base sequence of said amplified mitochondrial DNA; identifying sequence polymorphism at nucleotide base substitution loci in said non-coding region; and determining the lineage of said scallop from the sequence polymorphism identified at nucleotide base substitution loci in said non-coding region.