Patent ID: 9131669
Filing Date: 2015-09-15
Classification: A01K,A61P,C07K,C12N,C12P

Abstract:
1. A transgenic non-human mammal comprising a heterologous immunoglobulin heavy chain locus comprising human VH gene segments, one or more human D gene segments, human J gene segments, and rat constant region gene segments, wherein the human VH gene segments consist of VH1-69, VH4-59, VH3-53, VH3-49, VH4-34, VH3-48, VH3-33, VH3-30, VH3-23, VH1-18, VH3-15, VH4-39, VH1-8, VH3-07, VH2-5, VH4-4, VH1-2, and VH6-1, the human J gene segments comprise all six human J gene segments, and the rat constant region gene segments comprise at least Cμ and a Cγ and wherein said transgenic non-human mammal is capable of expressing a heavy chain from said heterologous heavy chain locus in response to antigen challenge, leading to the circulation of antigen-specific affinity matured, tetrameric immunoglobulins in the transgenic non-human mammal serum.