Patent ID: 7604964
Filing Date: 2009-10-20
Classification: C12Q

Abstract:
1. A method of determining the relative copy number (CN) of a first nucleotide sequence I (NucSeqI) in a test sample using an amplification technique, said method comprising the steps of: (1) adding to the test sample that comprises NucSeqI and a chromosome-derived second nucleotide sequence II (NucSeqII), the following ingredients: (2) carrying out the following amplification steps in one or more amplification cycles: (3) determining the results of the amplifications of step (2) expressed as threshold cycle (Ct) as a function of said starting quantity, concentration or dilution; (4) obtaining from the results in step (3) the following values: (5) determining from the values obtained in step (4) the relative CN of NucSeqI with respect to NucSeqII by the formula: thereby determining the relative CN of NucSeqI in said test sample.