Patent ID: 8691568
Filing Date: 2014-04-08
Classification: A61P,C12N

Abstract:
1. A method for preparing cell populations with anti-tumor immune response activity, wherein the method includes: (1) co-culturing tumor cells and mononuclear cells for 7-28 days in a three-dimensional cell culture device, thereby obtaining a culture which comprises cell populations with anti-tumor immune response activity; (2) separating the cell population with anti-tumor immune response activity from the culture which is obtained by step (1); wherein, the three-dimensional cell culture device includes: (a) a container with liquid cell culture medium; and (b) a three-dimensional cell culture unit in the liquid cell culture medium, the three-dimensional cell culture unit is formed of biodegradable material which melts at a temperature of about 50° C. to about 100° C. and are solid at a temperature of 25° C. to about 37° C.; comprises one or more empty cavities used for cell culture, wherein the empty cavity walls define the empty-cavities; the empty cavity walls are comprised of a biodegradable material to which cells can adhere and grow and the cavity walls are permeable to the nutritional substances and the products of cell metabolism; wherein the biodegradable material of the cavity walls of the three dimensional culture device is nonopaque and is at least one selected from the group consisting of agar, agarose, hydrogels, collagen, and matrigel; and wherein the tumor cells and mononuclear cells are put together in the one or more empty cavities of the three-dimensional cell culture unit within the cell culture medium thereby forming a co-culture of tumor cells and mononuclear cells resulting in a cell population with anti-tumor immune response activity.