Patent ID: 8420393
Filing Date: 2013-04-16
Classification: A61K,A61P,C12N,C12Y

Abstract:
1. A method for generating a library of stem cells comprising autologous stem cells as library members, wherein the stem cells are derived from parthenogenetically activated oocytes from one or more human donors, wherein each library member is homozygous for one or more genes selected from HLA DRB1, DRB3, DRB4, DRB5, DQA1, and DQB1 haplotype combinations, the method comprising: a) parthenogenetically activating a human oocyte, wherein activating comprises: i) contacting the oocyte with an ionophore at high O2 tension and ii) contacting the oocyte with a serine-threonine kinase inhibitor under low O2 tension; b) cultivating the activated oocyte of step (a) at low O2 tension until blastocyst formation; c) transferring the blastocyst to a layer of feeder cells, and culturing the transferred blastocyst under high O2 tension; d) mechanically isolating an inner cell mass (ICM) from trophectoderm of the blastocyst of step (c); and e) culturing the cells of the ICM of step (d) on a layer of feeder cells, wherein culturing step (e) is carried out under high O2 tension, thereby producing a library of human stem cells.