Patent ID: 9109010
Filing Date: 2015-08-18
Classification: A61P,B01D,C07K

Abstract:
1. A method for producing a host cell-protein (HCP) reduced antibody, or antigen binding portion thereof, preparation from a sample mixture comprising an antibody, or antigen binding portion thereof, an HCP and a viral particle, wherein said sample mixture has not been exposed to Protein A, and wherein the preparation comprises a decreased number of viral particles or decreased viral activity in comparison to the sample mixture, said method comprising: (a) subjecting said sample mixture to a reduction in pH thus forming a primary recovery sample, wherein said reduction in pH is to a pH of 3.8 and said reduction is achieved by the addition of citric or phosphoric acid; (b) adjusting said primary recovery sample to a pH of about 5.0 followed by; (c) applying said primary recovery sample to an ion exchange resin and collecting an ion exchange sample; (d) applying said ion exchange sample to a hydrophobic interactive chromatography (HIC) resin and collecting an HIC sample, wherein said HIC sample comprises said HCP-reduced antibody, or antigen binding portion thereof, preparation.