Patent ID: 6080839
Filing Date: 2000-06-27
Classification: C07D,G01N,Y02P

Abstract:
A labeling reactant of formula (I) suitable for labeling of a biospecific binding reactant using solid-phase synthesis ##STR6## wherein -A- is a bivalent aromatic structure capable of absorbing light or energy and transferring the excitation energy to a lanthanide ion after the product made by the said solid-phase synthesis has been released from the used solid support, deprotected and converted to a lanthanide chelate;-G- is a bridge replacing a hydrogen atom in A and is formed of one to ten moieties, each moiety being selected from the group consisting of phenylene, alkylene containing 1-12 carbon atoms, ethynediyl (--C.tbd.C--), ether (--O--), thioether (--S--), amide (--CO--NH-- and --NH--CO-- and --CO--NR""- and --NR""--CO--), ester (--COO-- and --OOC--), disulfide (--S--S--), diaza (--N.dbd.N--) and tertiary amine (--NR""-), wherein R"" represents an alkyl containing less than 5 carbon atoms;R is a protected amino acid residue --CH(NHX)COOH, where X is a transient protecting group, or its active ester, where said ester is an N-hydroxysuccinimido, p-nitrophenol or pentafluorophenol ester; andR' is --COOR'"" where R'"" is an alkyl of 1 to 4 carbon atoms, phenyl or benzyl, which phenyl or benzyl can be substituted or unsubstituted.