Patent ID: 6342588
Filing Date: 2002-01-29
Classification: A61K,A61P,C07K,C12N,G01N

Abstract:
A method of obtaining a peptide or polypeptide, the method comprisingproviding in a common medium: label molecules; a marker ligand able to bind a member of a specific binding pair (sbp), which specific binding pair has a first sbp member and a second sbp member, and wherein said marker ligand is able to bind said second sbp member; said second sbp member; an enzyme able to catalyze binding of said label molecules to proteins, peptides or ligands, said enzyme being conjugated to said marker ligand; causing or allowing binding of said marker ligand to said second sbp member; and causing or allowing binding of said label molecules to proteins, peptides or ligands in the vicinity of said marker ligand bound to said second sbp member thereby providing labeled proteins, peptides or ligands; wherein said proteins, peptides or ligands to which said label molecules bind comprise a sbp member (â€œfirst sbp memberâ€ ) which binds said second sbp member or comprise a sbp member (â€œfurther sbp memberâ€ ) which binds a protein, peptide or ligand in the vicinity of said second sbp member, and said first sbp member or said further sbp member is displayed on the surface of a virus particle; the method further comprising: isolating and/or purifying said particle from the medium following binding of said first sbp member to said second sbp member or binding of said further sbp member to a protein, peptide or ligand in the vicinity of said second sbp member, and labeling of said particle and/or said displayed first or further sbp member; obtaining nucleic acid from an isolated and/or purified particle; and producing by expression from nucleic acid with the sequence of said nucleic acid obtained from said particle or a sequence modified by the addition, insertion, deletion and/or substitution of one or more nucleotides an encoded peptide or polypeptide.