Patent ID: 6743624
Filing Date: 2004-06-01
Classification: C12N

Abstract:
A process for the continuous purification and concentration of leukocytes from a buffy coat fraction, characterized in that said process comprises the following steps:(a) separating plasma from the buffy coat fraction by filtration in order to achieve a filtered buffy coat fraction; (b) adding an aqueous solution, which is hypotonic in relation to plasma, to the filtered buffy coat fraction resulting from step (a), in order to achieve lysis of erythrocytes contained in the filtered buffy coat fraction; (c) mixing the filtered buffy coat fraction and the aqueous hypotonic solution from step (b) in a mixing device; (d) leading the mixture from step (c) through a retention vessel; (e) leading the mixture from step (d) through a centrifuge in order to separate the leukocytes; (f) collecting the separated leukocytes from step (e).