Patent ID: 7904250
Filing Date: 2011-03-08
Classification: G16B,Y10S

Abstract:
1. A method for identifying in a sample single nucleotide polymorphisms relative to a reference nucleotide at a position in a reference sequence, the method comprising the steps of: hybridizing the sample to a microarray of oligonucleotide probes, the microarray comprising, for each reference nucleotide, a first probe complementary to the reference sequence and second, third and fourth probes complementary to the reference sequence except for a non-matching nucleotide at the position, the four probes defining a position group at a physical space on the microarray, the nucleotide at the position on each probe in the position group being unique and being selected from the group consisting of A, G, C and T; determining the relative hybridization intensity of each probe in each position group; classifying each position group as a conformer if the highest intensity probe conforms to the reference sequence at the position, as a nonconformer if the highest intensity probe does not conform to the reference sequence at the position; mapping the conformers and nonconformers to their physical space on the microarray; identifying as possible SNPs only those nonconformers physically located adjacent on the microarray to conformers; and outputting the identification of possible SNPs to a user.