Patent ID: 7541152
Filing Date: 2009-06-02
Classification: B82Y,G01N

Abstract:
1. A method of generating light sufficient to excite fluorescence from a fluorescent indicator molecule directly attached to a target molecule bound to a probe molecule at a feature within an array, comprising: providing an array comprising a binding molecule and a probe molecule at a feature within an array; contacting a target molecule having a fluorescent indicator molecule directly attached thereto to said probe molecule such that said target molecule and said probe molecule are bound to each other at said feature; contacting a carrier molecule to said binding molecule such that said carrier molecule and said binding molecule are attached to each other at said feature; contacting a light-emitting substance to said carrier molecule, wherein the light-emitting substance is capable of producing light of sufficient intensity and wavelength to excite said fluorescent indicator molecule; causing the light-emitting substance to emit light, wherein said light emitted from said light-emitting substance is absorbed by said fluorescent indicator molecule thereby causing said fluorescent indicator molecule to emit light; and detecting said light emitted from said fluorescent indicator molecule directly attached to said target molecule.