Patent ID: 6958811
Filing Date: 2005-10-25
Classification: G02B

Abstract:
1. A method for optical detection of quantities of different dyes in a specimen by detecting the wavelength-dependent behavior of an illuminated specimen in an image generating arrangement using spectra which contain overlapping dye spectra from different dyes which are simultaneously examined, the wavelength-dependent behavior including the emission behavior, absorption behavior, fluorescence, luminescence, phosphorescence, enzyme-active light emission, naturally occurring dye fluorescence from fluorescent proteins or enzyme-active fluorescence of the illuminated specimen, comprising: determining at least one spectral centroid position for different dye spectra detected by a spectral detector wherein a characteristic dye spectra is detected for at least one image point of the specimen; and applying a characteristic spectral weighting function to the characteristic dye spectra that is detected to separate overlapping dye components and to calculate the intensity of each dye component per desired image point.