Patent ID: 7700354
Filing Date: 2010-04-20
Classification: C12Q,G01N,Y10T

Abstract:
1. A high-throughput assay method for rapidly identifying a population of primitive hematopoietic cells having a proliferative status suitable for transplantation into a patient, comprising the steps of: (a) providing a cell population comprising primitive hematopoietic cells; (b) incubating the primitive hematopoietic cell population in a cell growth medium comprising fetal bovine serum having a concentration of between 0% and about 30%, methyl cellulose having a concentration of between about 0.4% and about 0.7%, and in an atmosphere having between about 3.5% oxygen and 7.5% oxygen; (c) contacting the primitive hematopoietic cell population with a proliferation agent, the proliferation agent comprising one or more growth factors, one or more cytokines, or combinations thereof; (d) contacting the cell population with a reagent capable of reacting with ATP and generating luminescence in the presence of ATP; and (e) detecting luminescence generated by the reagent that reacted with the ATP in the primitive hematopoietic cell population, the level of luminescence indicating the amount of ATP in the primitive hematopoietic cell population, wherein the amount of ATP indicates the proliferative status of the primitive hematopoietic cells and wherein the proliferative status of the primitive hematopoietic cells indicates the suitability of the cell population for transplantation into a recipient patient.