Patent ID: 7169555
Filing Date: 2007-01-30
Classification: C12Q,Y10T

Abstract:
1. A method for identifying microbial DNA or RNA comprising: (a) providing a sample containing microbial DNA or RNA; (b) enriching ribosomal DNA or RNA in the sample by a PCR method wherein the distance between two primers is between 100 and 250 base pairs on a template to be amplified with the primers, the primers containing 16 to 25 nucleotides, one of said primers being selected from the group consisting of PLK2H (SEQ ID NO: 47), REViFL (SEQ ID NO: 2) and iFL (SEQ ID NO: 1); (c) preliminarily identifying at least one microorganism on the basis of the melting temperature of the resultant amplification product from step (b); (d) adding at least one labeled oligonucleotide probe to said enriched ribosomal DNA or RNA whereby temperature-dependent hybridization takes place, wherein the probe is selected from the group consisting of ISN2 (SEQ ID NO: 43), ISN2* (SEQ ID NO: 44), ISN (SEQ ID NO: 34), ISP2 (SEQ ID NO: 46), ISP2* (SEQ ID NO: 45) and ISP (SEQ ID NO: 33); (e) obtaining a melting curve for the resultant probe-enriched ribosomal DNA or RNA hybridization products; and (f) identifying said at least one microorganism from its melting curve.