Patent ID: 8841092
Filing Date: 2014-09-23
Classification: C07H,C07K,C12P

Abstract:
1. A method of synthesizing a sugar moiety A-nucleotide diphosphate, in-situ, from a biomolecule having a sugar moiety A, the method comprising the steps of: (a) incubating the biomolecule having the sugar moiety A with a nucleotide diphosphate in the presence of a glycosyltransferase capable of glycosylating the biomolecule that is selected from the group consisting of (b) isolating the sugar moiety A-nucleotide diphosphate from step (a), wherein the biomolecule is an enediyne, a vancomycin, a bleomycin, an anthracycline, a macrolide, a pluramycin, an aureolic acid, an indolocarbazole, an aminglycoside, a glycopeptide, a polyene, a coumarin, a benzoisochromanequinone, a calicheamicin, an erythromycin, an avermectin, an ivermectin, an angucycline, a cardiac glycoside, a steroid or a flavinoid.