Patent ID: 8815780
Filing Date: 2014-08-26
Classification: B01L,B82Y,C40B,G01N

Abstract:
1. A method of analyzing protoplasts comprising: providing a channel and an array of nanofibers within the channel, wherein a first portion of the array comprises carbon nanofibers and has a pitch between adjacent carbon nanofibers that immobilizes protoplasts; and a second portion of the array of nanofibers is separated from walls of the channel and provides at least one electrode; passing a fluid that is a suspension of protoplasts in a liquid medium, through the channel, wherein the fluid is traversed across the first portion of the array of carbon nanofibers before the second portion of the array of nanofibers, wherein as the fluid is traversed past the first portion of the array of carbon nanofibers, at least some of the protoplasts are immobilized by the first portion of the array of carbon nanofibers, wherein each immobilized protoplast wedges between and simultaneously contacts at least three carbon nanofibers of the first portion of the array; applying a stimuli to the immobilized protoplasts; and measuring emissions by the immobilized protoplasts in response to the stimuli with the at least one electrode that is provided by the second portion of the array of nanofibers.