Patent ID: 9134241
Filing Date: 2015-09-15
Classification: G01N,G02B

Abstract:
1. A fluorescence imaging system, comprising: a sample platform; and an illumination and detection system, including: an illumination system that provides a beam of excitation light from a source to a targeted area on the sample platform, wherein the targeted area includes a fluorescent material, and Wherein the excitation light includes light having an excitation wavelength of the fluorescent material; a sensor adapted to detect light and having an array of sensing locations; and imaging optics arranged and configured to image light emanating from the sample platform onto the sensor, wherein fluorescent light emitted from the fluorescent material in the targeted area is imaged onto a first portion of the sensor comprising a first plurality of the sensing locations and background light from a re ion proximal to the targeted area on the platform is simultaneously imaged onto a second portion of the sensor comprising a second plurality of the sensing locations different from the first plurality of the sensing locations, wherein the region proximal to the targeted area. on the platform is not directly illuminated by the beam of excitation light, and wherein the background light includes scattered excitation light, and wherein a second signal detected by the second portion of the sensor is subtracted from a first signal detected by the first portion of the sensor to produce a. background-reduced signal, wherein the first signal and the second signal are simultaneously detected.