Patent ID: 9174181
Filing Date: 2015-11-03
Classification: B01F,B01L,C12M

Abstract:
1. A disposable bioreactor ( 1 , 1 ′) for cultivation of cells in a nutrient medium, comprising a reagent container ( 2 , 2 ″) having an open upper end ( 4 ) that is sealable with a lid ( 3 ), the lid ( 3 ) being provided with at least one opening ( 19 ) that is covered by a membrane ( 21 ) for achieving a sterile exchange of air, the container ( 2 , 2 ″) further having a lower end ( 8 ) and a container side wall ( 6 ) extending between the upper and lower ends ( 4 , 8 ), a closed bottom wall ( 9 ) at the lower end ( 8 ) of the container side wall ( 6 ), the reagent container ( 2 , 2 ″) being subjectable to a shaking operation in a shaking apparatus, the container side wall ( 6 ) having a substantially cylindrical inner surface extending from the open upper end ( 4 ) to the lower end ( 8 ), the cylindrical inner surface ( 7 , 7 ′, 7 ″) being interrupted only by a baffle ( 10 , 10 ′, 14 , 14 ′) consisting of as a single continuous uninterrupted web extending helically from a lower position in proximity to the lower end ( 8 ) to an upper position in proximity to the open upper end ( 4 ), the web ( 10 , 14 ) having a cross-section with a convex curvature ( 11 ) directed away from the inner surface ( 7 , 7 ′, 7 ″) of the container wall ( 6 ) and configured as an elliptical, parabolic or circular section to avoid sharp edges that can impair cell division and damage cells.