Patent ID: 9133501
Filing Date: 2015-09-15
Classification: C12Q,G01N

Abstract:
1. A method for detecting at least one direct factor Xa inhibitor in a sample, comprising the steps of: (a) providing a sample containing at least one direct factor Xa inhibitor and a body fluid selected from the group consisting of serum and urine; (b) providing a composition containing factor Xa; (c) providing a composition containing a chromogenic substrate conjugated to a detectable substance selected from the group consisting of N-benzoyl-L-isoleucyl-L-glutamyl-L-glycyl-L-arginine-para-nitroaniline hydrochloride, Boc-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin hydrochloride, and 4-Nitrophenyl 4-guanidinobenzoate hydrochloride; (d) mixing the sample of step (a) with the composition of step (b) and the composition of step (c) under conditions which allow the binding of the at least one direct factor Xa inhibitor to factor Xa and which allow the factor Xa to release the detectable substance from the chromogenic substrate; (e) measuring the amount of released detectable substance; wherein the sample does not contain citrated blood plasma.