Patent ID: 6448091
Filing Date: 2002-09-10
Classification: C07H,C12Q,G01N

Abstract:
A method for performing a binding assay for an analyte of interest possibly present in a sample comprising:(a) forming a composition containing: (i) said sample; (ii) an assay-performance-substance comprising at least one compound selected from the group consisting of: (1) added analyte of interest or added analogue of said analyte; (2) a binding partner of said analyte or a binding partner of said analogue; and (3) a component capable of binding with (1) or (2); wherein said assay-performance-substance further contains a label compound linked to at least one of said (1), (2), and (3) and wherein said label compound is capable of chemiluminescing when triggered; and (iii) a plurality of particles capable of specifically binding with the analyte and/or said assay-performance-substance; (b) incubating said composition to form a complex which includes a particle and said labeled component; (c) introducing a suspension of said particles into a flow-cell; (d) collecting said particles in a collection zone contained within said flow-cell thereby removing said particles from suspension, wherein said composition is flowed through the flow-cell at a sufficiently low rate of flow to permit collection of at least a portion of said particles; (e) introducing into said collection zone a trigger capable of triggering said label such that said label chemiluminesces; and (f) measuring the emitted chemiluminescence from said label on said complex while said particles are collected at said collection zone to measure the presence of the analyte of interest in the sample.