Patent ID: 6280937
Filing Date: 2001-08-28
Classification: C12N

Abstract:
A method for screening for a nucleic acid that produces a preferred activity or phenotype in a mammalian cell, said method comprising the steps of:(i) transforming yeast with a shuttle vector comprising:(a) an origin of replication functional in yeast; and(b) a selectable gene functional in yeast; and(c) a promoter functional in a mammalian cell and capable of directing transcription of a polypeptide coding sequence operably linked downstream from said promoter; and(d) a homologous recombination site; and(ii) transforming yeast with a vector comprising a heterologous nucleic acid flanked by regions identical to said homologous recombination site wherein said heterologous nucleic acid comprises a nucleic acid of interest and a fusion partner; and(iii) allowing said shuttle vector to recombine so as to insert said heterologous nucleic acid into said shuttle vector at said homologous recombination site; and(iv) isolating said shuttle vector from said yeast; and(v) introducing said shuttle vector into a mammalian cell and expressing said heterologous nucleic acid therein; and(vi) assaying for said preferred activity or preferred phenotype in said mammalian cell.