Patent ID: 8716033
Filing Date: 2014-05-06
Classification: A61K,C07K,C12P,C12Y,G01N,Y10T

Abstract:
1. A method of producing a glycomodified protein, comprising attaching an azido-modified galactose to a GlcNAc residue on a first protein having a GlcNAc-GlcNAc linkage; and mixing said first protein, having attached thereto said azido-modified galactose with a reporter molecule, carrier molecule or solid support capable of reacting with the azide group of said azido-modified galactose; wherein said azido-modified galactose is N-azidoacetylgalactosamine (GalNAz) and said reporter molecule, carrier molecule or solid support is labeled with an alkyne or an activated alkyne group; wherein said reporter molecule, carrier molecule or solid support attaches to the first protein at the azide group of said attached azido-modified galactose through the alkyne or the activated alkyne group, thereby forming the glycomodified protein; and wherein said attaching of the azido-modified galactose (GalNAz) is carried out by an enzymatic attachment step utilizing a mutant β-1,4-galactosyl transferase enzyme (Y289L GalT1) and UDP-GalNAz as a substrate, which transfers said azido-modified galactose (GaINAz) from said UDP-GalNAz to said GlcNAc residue of said first protein.