Patent ID: 6180366
Filing Date: 2001-01-30
Classification: C12N,C12Y

Abstract:
A method for producing a heterologous polypeptide, comprising:(a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the heterologous polypeptide, wherein the mutant comprises a first nucleic acid sequence encoding the heterologous polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes involved in the production of a trichothecene selected from the group consisting of tri3, tri4, tri5, tri6, tri11, tri12, and tri101, such that the mutant produces less o the trichothecene than the parent filamentous fungal cell when cultured under the same conditions; and(b) isolating the heterologous polypeptide from the cultivation medium.