Patent ID: 6787327
Filing Date: 2004-09-07
Classification: C12N,C12Q

Abstract:
A method of determining tyrosine-DNA phosphodiesterase activity, comprising:a. providing: i. tyrosine-DNA phosphodiesterase: ii. p-nitrophenyl thymidine-3â€²-phosphate; and iii. manganese cation: b. combining said tyrosine-DNA phosphodiesterase, said p-nitrophenyl thymidine-3â€²-phosphate and said manganese cation under conditions wherein the tyrosine-DNA phosphodiesterase reacts with the p-nitrophenyl thymidine-3â€²-phosphate to release t,-nitrophenyl; and c. detecting said p-nitrophenyl, wherein said tyrosine-DNA phosphodiesterase is human tyrosine-DNA phosphodiesterase comprising amino acid sequence SEQ ID NQ:2.