Patent ID: 6649590
Filing Date: 2003-11-18
Classification: A21D,A23C,A23J,A23K,A23L,A61K,A61P

Abstract:
A method for producing a fraction containing milk basic cystatin or its decomposition product, comprising the steps of: (i) preparing a composition containing a basic protein fraction from milk; (ii) contacting said composition with a heparin-immobilized resin; (iii) eluting a fraction from the resin; (iv) subjecting said fraction to ultrafiltration using a membrane having a cut-off molecular weight of 10-50 kDa; (v) recovering a permeate from said ultrafiltration membrane to obtain a fraction containing milk basic cystatin, wherein said fraction has inhibitory activity on proteolytic activity of cysteine proteases and on virus growth; and optionally (vi) decomposing the permeate with a protease to obtain a decomposition product, wherein said protease is selected from the group consisting of trypsin, chymotrypsin, pepsin, papain, kallikrein, cathepsin, thermolysin, and V8 protease, and wherein the decomposition product has a bone resorption suppressing activity.