Patent ID: 6548253
Filing Date: 2003-04-15
Classification: C12N

Abstract:
A method of isolating plasmid DNA from a microorganism culture with the aid of solid-phase bodies, comprising:a) acidifying said culture to an acid pH and mixing said culture with solid-phase bodies which are underivatized with antibody and which nonspecifically immobilize microorganisms thereon, b) resuspending the immobilized microorganisms, lysing the microorganisms, adding a neutralising binding buffer and incubating the buffer with the lysed microorganisms, followed by removing and discarding the solid-phase bodies, c) mixing the supernatant from step (b), containing plasmid DNA, with solid-phase bodies and incubating the supernatant with the bodies followed by eluting the plasmid DNA from the solid-phase bodies with an elution buffer.