Patent ID: 6316409
Filing Date: 2001-11-13
Classification: A61K,A61P,C07K,C12N

Abstract:
A modified polypeptide ligand capable of binding a calcium dependent binding protein, which ligand comprises an amino acid sequence corresponding to that of a calcium dependent binding protein binding sequence of wild type ligand for the calcium dependent binding protein containing a substitution of at least one amino acid, which substitute amino acid has a hydrophobic side chain and substitutes into a helical peptide with minimal perturbation of the peptide structure, for a hydrophilic or aromatic amino acid, such that the affinity of the modified polypeptide ligand for the calcium dependent binding protein is enhanced, as determined by a reduction of the K.sub.d value or a reduction of the k.sub.off value, or both, relative to binding of the wild-type ligand to the calcium dependent binding protein.