Patent ID: 8703931
Filing Date: 2014-04-22
Classification: C07B,C12N,C12Q,Y10T

Abstract:
1. A method for purifying nucleic acids from a biological sample, comprising the following steps: a) collecting the sample in a first sample vessel in an aqueous solution and lysing the sample under nonchaotropic conditions; b) suspending first magnetic particles in the solution such that cellular debris including at least proteins and membrane components are separated from the solution by binding to the magnetic particles while the nucleic acids remain in the solution; c) placing the first sample vessel into a sample vessel holder, wherein the sample vessel is placed into the annular interior space of a ring magnet associated with the sample vessel holder; d) separating the solution from the magnetic particles, wherein cellular debris are separated from the solution via separation of the magnetic particles with the cellular debris bound thereto, the separated solution containing the nucleic acids to be isolated; and e) isolating the nucleic acids from the solution, wherein the solution is heated prior to step d) to at least 50° C.