Patent ID: 6346656
Filing Date: 2002-02-12
Classification: C07K,C12N

Abstract:
A method of increasing resistance to porphyrin-accumulating type herbicides of plant or algal cells compared to untransformed plant or algal cells, comprising introducing a DNA into said plant or algal cells so that said DNA is expressed in said plant or algal cells, wherein said DNA is one of the following:(A) an isolated, purified DNA, having the following characteristics: (i) said DNA comprises a nucleotide sequence of a DNA fragment having a molecular size of approximately 3.4 kb, said DNA fragment being obtained from a strain of the unicellular green alga Chlamydomonas reinhardtii that exhibits resistance to porphyrin-accumulating type herbicides, (ii) said DNA contains restriction sites for XhoI, PstI, PstI, PstI, PstI, PstI, BamHI, SalI, SalI, and XhoI, and has a restriction site map as shown in FIG. 1(a), and (iii) said DNA confers resistance to porphyrin-accumulating type herbicides in plant or algal cells when expressed therein; (B) an isolated, purified DNA having the following characteristics: (i) said DNA comprises a nucleotide sequence of a DNA fragment having a molecular size of approximately 9.9 kB, said DNA fragment being obtained from a strain of the unicellular green alga Chlamydomonas reinhardtii that exhibits resistance to porphyrin-accumulating type herbicides, (ii) said DNA contains restriction sites for EcoRI, XhoI, PstI, PstI, PstI, PstI, PstI, BamHI, SalI, SalI, XhoI, and HindIII, and has a restriction site map as shown in FIG. 1(b), and (iii) said DNA confers resistance to porphyrin-accumulating type herbicides in plant or algal cells when expressed therein; (C) an isolated, purified DNA, having the following characteristics: (i) said DNA comprises a nucleotide sequence of a DNA fragment having a molecular size of approximately 10.0 kB, said DNA fragment being obtained from a strain of the unicellular green alga Chlamydomonas reinhardtii that exhibits resistance to porphyrin-accumulating type herbicides, (ii) said DNA contains restriction sites for EcoRI, XhoI, PstI, PstI, PstI, PstI, PstI, BamHI, SalI, SalI, XhoI, HindIII, and KpnI, and has a restriction site map as shown in FIG. 1(c), and (iii) said DNA confers resistance to porphyrin-accumulating type herbicides in plant or algal cells when expressed therein; (D) an isolated, purified DNA, having the following characteristics: (i) said DNA comprises a nucleotide sequence of a DNA fragment having a molecular size of approximately 13.8 kB, said DNA fragment being obtained from a strain of the unicellular green alga Chlamydomonas reinhardtii that exhibits resistance to porphyrin-accumulating type herbicides, (ii) said DNA contains restriction sites for EcoRI, XhoI, PstI, PstI, PstI, PstI, PstI, BamHI, SalI, SalI, XhoI, HindIII, BamHI, SalI, HindIII, and KpnI, and has a restriction site map as shown in FIG. 1(d), and (iii) said DNA confers resistance to porphyrin-accumulating type herbicides in plant or algal cells when expressed therein; or (E) an isolated, purified DNA comprising the nucleotide sequence shown in SEQ. ID. NO.: 1.