Patent ID: 6632629
Filing Date: 2003-10-14
Classification: B01L,C12Q,G01N

Abstract:
A method for screening a plurality of compounds for modulators that affect the activity of a protease, the method comprising:(i) providing a plurality of compounds; (ii) introducing a compound selected from the plurality of compounds into a microscale channel; (iii) contacting the compound with a protease and a protease substrate in the microscale channel, thereby reacting the protease substrate with the protease in the presence of the compound to produce one or more products; (iv) labeling the one or more products by flowing a labeling reagent into the at least one microscale cavity or into the additional microscale cavity to react with the one or more products and produce one or more labeled products; (v) detecting the amount of one or more of the labeled products to determine the level of inhibition or activation of the compound; and (vi) repeating steps (ii) through (v) for each of the plurality of compounds.