Patent ID: 7767389
Filing Date: 2010-08-03
Classification: G01N

Abstract:
1. A method for evaluating quantitative changes in barrier properties on one or more affected surfaces of epithelial tissue of an animal subject caused by a test product, comprising the steps of: (a) harvesting one or more control surfaces of epithelial tissue from said animal subject to collect a first portion of mixed analytes as a control sample; (b) harvesting said affected surface of epithelial tissue from said animal subject of step (a) to collect a second portion of mixed analytes as a test sample; (c) identifying a marker analyte selected from at least one of albumin, keratin 1, keratin 10, keratin 11, cholesterol and biological modifications of cholesterol, intergrins, loricrin, pancornulin, arachodonic acid and its metabolites, and trichohyalin, in said test and control samples; (d) evaluating the effect of said test product on the epithelial tissue by evaluating said quantitative changes on said affected areas of the epithelial tissue by comparing the marker analyte from said affected areas with the marker analyte from said control area.