Patent ID: 8175809
Filing Date: 2012-05-08
Classification: G01N

Abstract:
1. A fluorescence analyzing method comprising the steps of: irradiating a board, to which a plurality of oligonucleotides are fixed, with light for fluorescence measurement; focusing produced fluorescence to form an image; and detecting the fluorescence with a two-dimensional sensor, wherein: the board is provided with multiple regions to each of which is fixed one of the plurality of oligonucleotides, each region is spaced apart from an adjacent region on the board equidistantly in the vertical and horizontal directions, the following equation is satisfied: where ds denotes the distance between two adjacent regions, M denotes the imaging magnification of an optical focusing/imaging system, dd denotes the pixel pitch of the two-dimensional sensor, and n denotes an integer (n=1, 2, 3, 4, 5), and ds is between 100 nm and 10000 nm.