Patent ID: 8937756
Filing Date: 2015-01-20
Classification: C12Q,G02B,G03H

Abstract:
1. A method for detecting molecules or structures stained or labelled to at least one cell or conjugated to antibodies which are bound either directly to said at least one cell or indirectly via another or several antibodies in a chain bound to said at least one cell, the molecules or structures being capable of shifting the polarization direction of a beam of light, the method comprising using a digital holographic microscopy and imaging set up to detect the molecules or structures including the steps of: performing digital holographic microscopy and imaging on a cell sample by a) creating at least one object beam and at least one reference beam of light, where said at least one object beam and said at least one reference beam are mutually coherent; b) shifting the polarization direction of the at least one object beam in relation to the at least one reference beam by shifting the polarization direction of the at least one object beam and/or the at least one reference beam; c) exposing said cell sample to said at least one object beam, wherein the polarization direction of said at least one object beam is shifted by utilization of said molecules or structure; d) superimposing said at least one object beam that has passed through said cell sample with said at least one reference beam and thereby creating an interference pattern; e) detecting said interference pattern; f) reconstructing phase and/or amplitude information of object wavefront from said interference pattern; and g) constructing at least one cell analysis image for detection of molecules or structures stained or labelled to at least one cell or conjugated to antibodies which are bound either directly to said at least one cell or indirectly via another or several antibodies in a chain bound to said at least one cell.