Patent ID: 7462760
Filing Date: 2008-12-09
Classification: C07K,C12N,Y02A

Abstract:
1. A method for protecting a Bacillus thuringiensis Cry8Bb 1 toxin or a variant thereof from proteolytic inactivation in a plant, wherein said variant has pesticidal activity and at least 99% sequence identity to the amino acid sequence set forth in SEQ ID NO: 22 for said Bacillus thuringiensis Cry8Bb1 toxin, said method comprising replacing at least one proteolytic site within said Bacillus thuringiensis Cry8Bb 1 toxin or variant thereof that is sensitive to a plant protease to comprise a proteolytic protection site, wherein said proteolytic protection site is not sensitive to said plant protease and protects said Bacillus thuringiensis Cry8Bb 1 toxin or variant thereof from proteolytic inactivation in a plant, and wherein the proteolytic protection site is the amino acid sequence set forth in SEQ ID NO: 143 and replaces the proteolytic site set forth in SEQ ID NO: 142.