Patent ID: 6274325
Filing Date: 2001-08-14
Classification: G01N

Abstract:
A method for determining an analyte in a homogeneous immunoassay, consisting essentially of:(i) reacting a Fab' molecule with biotin, wherein said Fab' molecule is derived from an IgG molecule and has from 1-3 free SH groups in its hinge region and said biotin is either coupled to said free SH groups via a functional group attached to said biotin or via a spacer group coupled to biotin via a functional group, wherein the spacer group comprises an alkylene chain of from 4 to 16 carbon atoms to obtain an IgG derived Fab'-biotin conjugate or an IgG derived, Fab'-spacer biotin conjugate,(ii) adding said conjugate to a sample together with an agglutinatole particle coated with avidin or streptavidin, and(iii) determining agglutination as a determination of said analyte in said sample.