Patent ID: 7452665
Filing Date: 2008-11-18
Classification: C12N,C12Q,Y10S

Abstract:
1. A kit for the synthesis of a polynucleotide, said kit comprising A mixture comprising: (a) a first DNA polymerase, wherein said first polymerase possesses 3′-5′ exonuclease activity selected from the group consisting of Archaebacterial DNA polymerases, and (b) a second DNA polymerase, wherein said second polymerase lacks 3′-5′ exonuclease activity selected from the group consisting of thermostable DNA polymerases lacking 3′-5′ exonuclease activity; wherein the ratio of DNA polymerase activity of the first DNA polymerase to the DNA polymerase activity of the second DNA polymerase is from about 1:100 up to about 1:600.