Patent ID: 6472150
Filing Date: 2002-10-29
Classification: C07K

Abstract:
A method of screening a nucleic acid library for a biomolecule that kills an arthropod, comprising the steps of:(a) feeding an array of elements to a population of arthropods, wherein each element of said array contains a clone or mixture of clones of said nucleic acid library, wherein individual arthropod mainly feeds on a single element but has the opportunity to feed upon any element in the array; (b) isolating dead arthropods from said population of arthropods after feeding on said array; (c) preparing midgut samples from said dead arthropods; and (d) recovering a clone or mixture of clones of said nucleic acid library from said midgut samples of said dead arthropods, wherein said clone or mixture of clones encode a biomolecule that kills said arthropod.