Patent ID: 7968313
Filing Date: 2011-06-28
Classification: C07K,C12N

Abstract:
1. A method of selecting cells expressing heteromeric immunoglobulin heavy and light chain complex comprising transfecting cells with a vector comprising a first nucleic acid encoding a first immunoglobulin heavy chain or immunoglobulin light chain, wherein the transcription of said first nucleic acid is operably linked to transcription of a second nucleic acid encoding a first subunit of a selectable marker, and further comprising a third nucleic acid encoding a second immunoglobulin light chain or immunoglobulin heavy chain wherein the immunoglobulin light chain is capable of associating with the first immunoglobulin heavy chain to form a heteromeric complex, wherein the transcription of said third nucleic acid is operably linked to transcription of a fourth nucleic acid which encodes a second subunit of a selectable marker, and wherein said second subunit associates with the first subunit of the selectable marker, to provide a selectable activity, and selecting the cells under conditions for expression of the immunoglobulin heavy and light chain complex.