Patent ID: 6426224
Filing Date: 2002-07-30
Classification: A61K,B01J,C07K,C12N

Abstract:
A method of recombining an oligonucleotide set, the method comprising:aligning a plurality of homologous nucleic acid sequences to identify one or more regions of sequence heterogeneity; synthesizing a plurality of different oligonucleotide member types which correspond to one of the regions of heterogeneity; mixing the plurality of different oligonucleotide member types, thereby providing a set of oligonucleotides which comprise a plurality of different oligonucleotide members which comprise the at least one regions of sequence heterogeneity which corresponds to one or more of the regions of heterogeneity in the plurality of homologous nucleic acid sequences; and, recombining one or more member of the oligonucleotide set with one or more nucleic acid corresponding to one or more of the homologous nucleic acid sequences.