Patent ID: 8951940
Filing Date: 2015-02-10
Classification: C12Q

Abstract:
1. A method of fabricating an affinity binding patch on a bead comprising: (a) providing a bead comprising a plurality of first polynucleotides attached to the surface of said bead, wherein the first polynucleotides each comprise a capture sequence; (b) providing a solid surface comprising a plurality of second polynucleotides attached to said solid surface, wherein said second polynucleotides each comprise a capture region and a linker region, the capture region comprising a capture-complement sequence and an affinity ligand, and the linker region comprising a cleavable moiety, wherein said affinity ligand is selected from the group consisting of: biotin, imino-biotin, an antibody, a functional antibody fragment, a peptide and a peptide nucleic acid; (c) hybridizing said capture sequences of said first polynucleotides to said capture-complement sequences of said second polynucleotides, thereby forming an immobilized bead on said solid surface, and (d) cleaving said second polynucleotides at said cleavable moiety so as to retain said affinity ligand on said second polynucleotides so that said affinity ligand is transferred to said bead, thereby fabricating an affinity binding patch on said bead.