Patent ID: 7732169
Filing Date: 2010-06-08
Classification: C12Q

Abstract:
1. A method of detecting the presence of West Nile virus in a test sample, comprising the steps of: (a) performing an in vitro nucleic acid amplification reaction to synthesize an amplicon comprising a West Nile virus nucleic acid sequence, said in vitro nucleic acid amplification reaction comprising extension of a first primer by a DNA polymerizing enzyme to synthesize a first primer extension product using as a template any West Nile virus polynucleotides contained in said test sample, (b) hybridizing to said first primer extension product and to said amplicon an oligonucleotide up to 37 nucleotides in length that comprises 13-37 contiguous bases of SEQ ID NO:95, allowing for RNA and DNA equivalent bases, to form a hybridization product; and (c) detecting the hybridization product as an indication of the presence of West Nile virus in the test sample.