Patent ID: 8617881
Filing Date: 2013-12-31
Classification: A61P,C07K,C12N

Abstract:
1. A method for producing a polyclonal cell population, said method comprising the steps of: a) transforming one or more host cells with at least one vector comprising at least one copy of a nucleic acid sequence that encodes a component of a polyclonal protein composition, in the presence of a recombinase or integrase, such that the at least one vector integrates into the host cell genome at a plurality of locations recognized by the recombinase or integrase; b) generating a cell population, without clonal selection, capable of producing the component of the polyclonal protein composition; and c) mixing the cell population and a different cell population producing a different component of the polyclonal protein composition generated by performing steps (a) and (b), each cell population comprising greater than about 10,000 individual cells to produce a polyclonal cell population capable of producing the polyclonal protein composition.