Patent ID: 7888291
Filing Date: 2011-02-15
Classification: A61P,C07K,C12Q,G01N

Abstract:
1. A method for assaying for modulators of β-secretase activity, comprising: (a) contacting a polypeptide with β-secretase APP processing activity with a substrate, both in the presence and in the absence of a putative modulator compound; wherein said substrate comprises a peptide having an amino acid sequence of at least 6 amino acids, said amino acid sequence including four amino acids defined by formula P P P P P wherein the substrate is cleaved between P wherein said peptide does not comprise the corresponding P (b) measuring cleavage of the substrate peptide in the presence and in the absence of the putative modulator compound; and (c) identifying modulators of β-secretase activity from a difference in substrate cleavage in the presence versus in the absence of the putative modulator compound, wherein a modulator that is a β-secretase antagonist reduces such cleavage and a modulator that is a β-secretase agonist increases such cleavage.