Patent ID: 7527954
Filing Date: 2009-05-05
Classification: C12N

Abstract:
1. A method for the production and allocation of nucleic acids and the polypeptides coded by these, comprising the following steps: a) the compartmentalisation of nucleic acids together with an in vitro transcription-translation mixture in a water-in-oil emulsion, b) the in vitro expression of fusion polypeptides coded by said nucleic acids in the microcompartments of the water-in-oil emulsion, whereby each nucleic acid is bonded to the fusion polypeptide it codes for, wherein each of the fusion polypeptides comprises at least one constant peptide part I and at least one variable peptide part II, and wherein the fusion polypeptides are covalently bonded to the nucleic acid coding for said fusion polypeptide in step b), and wherein the number of the fusion polypeptides per nucleic acid bonded in this manner is a definable integer.