Patent ID: 6140108
Filing Date: 2000-10-31
Classification: C12N,Y10S

Abstract:
A method for making an improved starter wine yeast culture, which culture comprises an improved trait that can be introduced by breeding, said method comprising steps:a) assembling and surveying a population of natural wine yeast for an improved trait;b) selecting from said population yeast cells comprising said improved trait;c) crossing said cells to a well-established commercial strain by:i) selecting HO/HO spores from said cells and said commercial strain;ii) treating resultant asci with zymolase and carrying out spore-spore pairings;iii) observing the spore pairs at intervals to detect formation of primary zygotes;iv) selecting and sporulating primary zygote clones;d) analyzing segregants of said primary zygote clones and scoring said clones for said trait;e) selecting segregants presenting most improvement in said trait;f) repeating crossing step c) using a different HO/HO spore clone from the commercial parent in each cross; wherein a culture comprising said improved trait is obtained.