Patent ID: 6485944
Filing Date: 2002-11-26
Classification: B01J,C12Q

Abstract:
A method of amplifying a plurality of nucleic acids, said method comprising the steps of:a) creating an array of immobilized oligonucleotide primers, wherein the primers are immobilized on a semi-solid support; b) hybridizing amplification template to immobilized oligonucleotide primers, c) extending the immobilized oligonucleotide primers using a DNA polymerase activity, and deoxynucleotide triphosphates to form immobilized nucleic acid strands and then denaturing the amplification template from the immobilized nucleic acid strands; d) hybridizing non-immobilized oligonucleotide primers to the immobilized nucleic acid strands and extending :the non-immobilized oligonucleotide primers and denaturing the extended nonimmobilized oligonucleotide primers from the immobilized nucleic acid strands, and e) repeating steps (b) and (c) and (d) for a defined number of cycles to yield a plurality of amplified nucleic acid molecules.