Patent ID: 6881557
Filing Date: 2005-04-19
Classification: C07K

Abstract:
1. A method of making a humanized antibody comprising, obtaining a peptide sequence for a subject variable region encoded by a non-human mature antibody gene; identifying a first set of canonical CDR structure types for at least two CDRs within the non-human antibody variable region; obtaining a library of peptide sequences for human antibody variable regions for human antibodies selected from the group consisting of human antibody segments encoded by a human germline gene and a mature antibody gene; identifying a second set of canonical CDR structure types for at least two CDRs for each peptide sequence within the library of human variable region sequences; selecting a subset of member peptide sequences from the library by comparing the first set of canonical CDR structure types to the second set of canonical CDR structure types and selecting those human peptide sequences where the second set of canonical CDR structure is the same as the first set of canonical CDR structure types for CDR sequences at corresponding locations within the non-human and human variable regions, respectively; and constructing a chimeric molecule that includes each of the CDR sequences from the non-human variable region and framework regions from at least one member of the selected subset of human variable region peptide sequences, so that the chimeric molecule contains each of the non-human CDR sequences substituted for each of the human CDR sequences at corresponding locations in the variable region, and the framework sequence of the chimeric molecule differs from the human framework sequence of the at least one selected member peptide sequences by no more than 10 amino acid residues and the chimeric molecule binds an antigen that is the same as that bound by the subject antibody variable region encoded by the non-human mature antibody gene.