Patent ID: 8932830
Filing Date: 2015-01-13
Classification: A61K,A61P,C07K,C12N,C12Y

Abstract:
1. A method of making a biologically active chimeric protein comprising: a) transfecting a cell with (i) a first DNA construct comprising a DNA molecule encoding a first polypeptide comprising a biologically active molecule fused to at least a portion of an immunoglobulin constant region and (ii) a second DNA construct comprising a DNA molecule encoding a second polypeptide comprising at least a portion of an immunoglobulin constant region without a biologically active molecule or variable region of an immunoglobulin; b) culturing the cell under conditions such that the first polypeptide encoded by said first DNA construct and the second polypeptide encoded by said second DNA construct are expressed; and c) isolating dimers comprising the first polypeptide and the second polypeptide from said transfected cell.