Patent ID: 9140711
Filing Date: 2015-09-22
Classification: G01N

Abstract:
1. Method for a determination of vitamin D metabolites in a sample of body fluid whose content in vitamin D metabolites is to be determined, comprising the pre-analytical steps: (i) providing of a predetermined amount of a bibulous solid sorption material capable of adsorbing a sample of body fluid after application which sorption material has been selected for not becoming affected by a treatment with protic organic solvents, for not releasing any analytically interfering substances and for providing interactions for a binding of proteins on its surface; (ii) applying of a predetermined amount of body fluid onto the sorption material and adsorbing and drying of the liquid sample on the sorption material so that the proteins contained in the liquid sample are bound to the sorption material; (iii) storing or transporting of the sorption material with the sorbed liquid sample in a protective enclosure under protective conditions until quantitative determination of vitamin D metabolites, wherein protection against contact, moisture and/or light exposure is provided; and (iv) determining the vitamin D metabolites in the sorbed liquid sample.