Patent ID: 6770465
Filing Date: 2004-08-03
Classification: C12N,C12P

Abstract:
An engineered &bgr;-Ketoacyl-acyl carrier protein synthase protein comprising an amino acid sequence (i) wherein said amino acid sequence of said engineered &bgr;-Ketoacyl-acyl carrier protein synthase protein has a substitution of at least one amino acid residue selected from the group consisting of amino acid residues at a position corresponding to residue 108, 111, 114, 133, and 197 of SEQ ID NO: 47, and (ii) wherein said substitution is of a hydrophobic residue to a smaller residue, and (iii) wherein said engineered &bgr;-Ketoacyl-acyl carrier protein synthase protein increases long chain fatty acid accumulation compared to a &bgr;-Ketoacyl-acyl carrier protein synthase protein having at least one unaltered amino acid residue selected from the group consisting of amino acid residues at a position corresponding to residue 108, 111, 114, 133, and 197 of SEQ ID NO: 47.