Patent ID: 7381567
Filing Date: 2008-06-03
Classification: C12Q,G01N,Y10S,Y10T

Abstract:
1. A bioaffinity assay using lanthanide ions or their chelates as labels and dissociative fluorescence enhancement as a tool for detection comprising the steps of a) mixing a sample comprising an analyte to be assayed with reactants of said assay to obtain an assay mixture; b) reacting said analyte with said reactants, wherein a bioaffinity reaction between said analyte and said reactants of said assay takes place, resulting in a reaction product wherein wherein the lanthanides of i) and ii) above are hereinafter referred to as label lanthanides, c) separating said reaction product obtained in step b), said reaction product comprising said label lanthanides defined above, from the unbound free labeled reactants, d) performing a dissociative fluorescence enhancement which consists essentially of adding an enhancement solution having a pH of from 2.0 to 2.8 to e) measuring the amount, directly or inversely correlating with the amount of analyte of the sample in step a) of the label lanthanides of step d) as β-diketone complexes by fluorometry, wherein said enhancement solution comprises a β-diketone of formula I wherein R