Patent ID: 7772008
Filing Date: 2010-08-10
Classification: G01F,G01N

Abstract:
1. A method for determining the volume of a liquid aliquot, comprising the steps of: a. making a sample solution by adding a first dye to a solvent, the first dye having an extinction coefficient such that the sample solution has an absorbance value at a first wavelength which is different from an absorbance value at a second wavelength, wherein the sample solution made includes no other dye; b. making a diluent solution by adding a second dye to a solvent, the second dye having an extinction coefficient such that the diluent solution has an absorbance value at the first wavelength which is different from an absorbance value at the second wavelength, wherein the second dye is different from the first dye and wherein the diluent solution made includes no other dye; c. measuring the absorbance value of a known volume of the diluent solution at the first wavelength and at the second wavelength; d. adding an aliquot of the sample solution containing the first dye only to the known volume of diluent solution of step (c) to make a sample-diluent solution; e. measuring the absorbance value of the sample-diluent solution of step (d) at the first wavelength and at the second wavelength; and f. calculating the volume of the aliquot of the sample solution by using the absorbance values measured in step (c) and step (e).