Patent ID: 6670131
Filing Date: 2003-12-30
Classification: C12Q,Y02A

Abstract:
A method of electrochemically detecting presences of nucleic acids each having a particular sequence in a plurality of target nucleic acids, comprising:preparing first and second vessels, wherein in the second vessel, a solution of a nucleic acid binding reagent is dispensed, and at least the second vessel has a counter electrode on a bottom surface or an inside surface thereof; inserting the target nucleic acids into the first vessel; inserting to the first vessel a nucleic acid immobilized electrode having a stick-like-shape, on which multiple electrodes are formed, where multiple nucleic acid probes are immobilized to the multiple electrodes and the multiple electrodes are connected to an electric-circuit; making a hybridization reaction to occur in the first vessel; extracting the nucleic acid immobilized electrode from the first vessel and inserting the electrode into the second vessel; applying a predetermined voltage between the nucleic acid immobilized electrode and the counter electrode so as to measure electrochemical signals generated due to the nucleic acid binding reagent solution; and selecting one of the electrochemical signals by the electric-circuit to determine one of the plurality of target nucleic acids.