Patent ID: 8921046
Filing Date: 2014-12-30
Classification: C07K,C12Q

Abstract:
1. A method of generating redundant sequence information from a single template nucleic acid molecule using multiple sequencing enzymes, the method comprising: attaching the single template nucleic acid molecule to a solid surface; sequencing a polynucleotide region of the single template nucleic acid molecule with a first single sequencing enzyme to obtain a first polynucleotide sequence read of the polynucleotide region; exchanging the first single sequencing enzyme with a second single sequencing enzyme, wherein the first single sequencing enzyme is a first type of polymerase enzyme and the second single sequencing enzyme is a second type of polymerase enzyme that is different from the first type of polymerase enzyme; and sequencing the polynucleotide region of the single template nucleic acid molecule with the second single sequencing enzyme to obtain a second polynucleotide sequence read of the polynucleotide region, wherein the first and second sequence reads comprise redundant sequence information for the polynucleotide region, thereby generating redundant sequence information from the single template nucleic acid molecule.