Patent ID: 6054271
Filing Date: 2000-04-25
Classification: C07F,G01N

Abstract:
A method of labeling a carrier molecule, comprising: a) providing a bonding partner comprising said carrier molecule and a target sequence, andb) contacting said bonding partner with a biarsenical molecule under conditions wherein said biarsenical molecule specifically reacts with said target sequence,said target sequence comprising one or more cysteines capable of specifically reacting with said biarsenical molecule, said biarsenical molecule having the following formula: ##STR12## and tautomers, anhydrides, and salts thereof; whereineach X.sup.1 or X.sup.2, independently, is Cl, Br, I OR.sup.a, or SR.sup.a, orX.sup.1 and X.sup.2 together with the arsenic atom form a ring having the formula: ##STR13## R.sup.a is H, C.sub.1 -C.sub.4 alkyl, CH.sub.2 CH.sub.2 OH, CH.sub.2 COOH or CN; Z is 1,2-ethanediyl, 1,2, benzenediyl, 4-methyl-1,2-benzyenediyl, 1,2-cyclopentanediyl, 1,2-cyclohexanediyl, 3-hydroxy-1,2-propanediyl, 3-sulfo-1,2-propanediyl, or 1,2-bis(carboxy)-1,2-ethanediyl;Y.sup.1 and Y.sup.2, independently, are H or CH.sub.3 ; orY.sup.1 and Y.sup.2, together form a ring such that the biarsenical molecule has the formula: ##STR14## where M is O, S, CH.sub.2, C(CH.sub.3).sub.2, or NH; R.sup.1 and R.sup.2, independently, are OR.sup.a, OAc, NR.sup.a R.sup.b, or H;R.sup.3 and R.sup.4, independently, are H, F, Cl, Br, I, OR.sup.a, or R.sup.a ; orR.sup.1 together with R.sup.3, or R.sup.2 together with R.sup.4, or both, form a ring in which(i) one of R.sup.1 or R.sup.3 is a alkyl comprising two or three carbon atoms attached to the other, wherein the other is NR.sup.a and(ii) one of R.sup.2 and R.sup.4 is a alkyl comprising two or three carbon atoms attached to the other, wherein the other is NR.sup.a ;R.sup.b is H, C.sub.1 -C.sub.4 alkyl, CH.sub.2 CH.sub.2, CH.sub.2 COOH or CN;Q is CR.sup.a R.sup.b, CR.sup.a OR.sup.b, C.dbd.O, or a spirolactone having the formula: ##STR15## wherein the Spiro linkage is formed at C.sub.1.