Patent ID: 6531644
Filing Date: 2003-03-11
Classification: A01K,C12N

Abstract:
A method of identifying an anti cell proliferation drug target comprising:(a) providing a Drosophila or C. elegans that has been recombinantly modified to have altered expression of one or more sensitizer genes wherein the sensitizer gene or genes is selected from the group consisting of a tumor suppressor gene, an ortholog of a tumor suppressor gene, an oncogene, and an ortholog of an oncogene, limited to a portion of a target tissue dispensable for viability, wherein said limited portion of the target tissue is identifiable by presence or absence of expression of a marker gene; (b) generating progeny of the Drosophila or C. elegans that have mutations in, reduced expression, expression of putative interactor genes; (c) identifying progeny generated in (b) that exhibit a sensitizer gene-specific antiproliferation phenotype in the limited portion of the target tissue; and (d) identifying as an anti cell proliferation drug target, an interactor gene from a progeny identified in (c) that when mutated or underexpressed, results in the sensitizer gene-specific antiproliferation phenotype.