Patent ID: 7074398
Filing Date: 2006-07-11
Classification: A61K,C07K,C12N

Abstract:
1. A method for producing a recombinant retroviral particle encoding an SDI-1 polypeptide or a fragment thereof, wherein the SDI-1 polypeptide or fragment thereof inhibits cell proliferation, the method comprising: (a) stably transfecting an isolated producer cell line with a retroviral vector comprising in 5′ to 3′ order: (b) producing said recombinant retroviral particle in said stably transfected isolated producer cell line, wherein said retroviral particle encodes said SDI-1 polypeptide or fragment thereof, and further wherein said regulatory element or promoter becomes operatively linked to said nucleic acid sequence and regulates expression of said nucleic acid sequence upon infection of a target cell by said recombinant retroviral particle.