Patent ID: 6071725
Filing Date: 2000-06-06
Classification: C07K,C12N,C12Y

Abstract:
A method for associating foreign protein with a surface of a cell comprising:providing a recombinant vector containing nucleic acid encoding the foreign protein fused to nucleic acid encoding a protein selected from the group consisting of ice nucleation protein (INP) and partial ice nucleation protein (partial INP) wherein INP includes N-terminal unique amino acids, a central repeating region and C-terminal unique amino acids, and partial INP includes N-terminal unique amino acids and C-terminal unique amino acids without a central repeating region;transforming a gram negative bacterium with the recombinant vector to provide a transformant; andculturing the transformant to provide a fusion protein which includes the N-terminus of the foreign protein fused to one of the C-terminal unique amino acids of INP or partial INP.