Patent ID: 8597884
Filing Date: 2013-12-03
Classification: C12N,C12Q

Abstract:
1. A method of determining the efficacy of enzymatic cell lysis, the method comprising: providing an internal control essentially free of contaminating extracellular DNA comprising a plurality of bacterial cells that have been fixed with a fixing compound and have been dried; mixing the internal control with a sample, lysing the internal control and the sample with a lysing agent, wherein said lysing agent comprises an enzymatic lysing agent; and detecting a lysis product of the internal control, wherein said plurality of bacterial cells essentially free of contaminating extracellular DNA are prepared from a population of bacterial cells wherein the growth of said population of bacterial cells is synchronized to generate a morphologically homogeneous population at the time of harvest, wherein said time of harvest is during vegetative cell growth, and wherein said plurality of bacterial cells are Gram-positive cells.