Patent ID: 6242592
Filing Date: 2001-06-05
Classification: A61K,C07H,C07J,C12N,C12Q,C12Y

Abstract:
A process for preparing an oligonucleotide that comprises at least one 2'-O-alkylated cytidine nucleotide comprising:alkylating a cytidine to form a 2'-O-alkylated cytidine;blocking the 5'-hydroxyl moiety of said 2'-O-alkylated cytidine;phosphitylating the 3'-position of said 5'-blocked 2''-alkylated cytidine to form a 2'-O-alkylated cytidine 3'-O-phosphoramidite; andcoupling said 2'-O-alkylated cytidine 3'-O-phosphoramidite with a 5'-hydroxyl moiety on an oligonucleotide utilizing phosphoramidite coupling conditions.