Patent ID: 6312912
Filing Date: 2001-11-06
Classification: C07K,C12N,C12Q

Abstract:
A method for identifying a cis-acting RNA export element comprising:measuring expression of a heterologous nucleic acid sequence in the presence and absence of an operably associated first RNA export element, which first RNA export element comprises one or more sub-elements selected from the group consisting of WPRE.alpha., WPRE.beta., and PRE.gamma., and exoression of a heterologous nucleic acid sequence in the presence and absence of an operably associated putative RNA export element, andcomparing the expression of the heterologous nucleic acid sequence in the presence and absence of the operably associated putative RNA export element with the expression of the heterologous nucleic acid sequence in the presence and absence of the operably associated first RNA export element, wherein the putative RNA export element is identified as a cis-acting RNA export element when the expression of the heterologous nucleic acid sequence measured in the presence, relative to the absence, of the operably associated putative RNA export element is equal to or higher than the expression of the heterologous nucleic acid sequence measured in the presence, relative to the absence, of the operably associated first RNA export element.