Patent ID: 7846748
Filing Date: 2010-12-07
Classification: G01N,Y10S,Y10T

Abstract:
1. A method of quantifying the amount or concentration of a peptide in two different samples, the method comprising the steps of: (a) providing a first sample containing an amount of a peptide and a second sample containing an amount of the peptide, the peptide in the first sample and the peptide in the second sample being differentially isotopically-labeled such that the peptide in the first sample has a different molecular weight than the peptide in the second sample, the peptide having a continuous epitope, wherein the peptide comprises 2 to 22 amino acids; (b) providing a bead having a volume and having a plurality of antibodies immobilized thereon, each of the antibodies having a paratope specific to the continuous epitope of the peptide; (c) positioning the isotopically-labeled peptides for analysis by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), comprising: (d) analyzing the differentially isotopically-labeled peptides using MALDI-MS to determine the relative amounts or concentrations of the peptide in the first sample and the second sample.