Patent ID: 6855704
Filing Date: 2005-02-15
Classification: A61K,C07J

Abstract:
1. A method for obtaining crude conjugated estrogens from pregnant mare urine containing major conjugated estrogen components and minor conjugated estrogen components comprising: (a) contacting a mare urine material having a pH of about 8.5 to about 9.5 and freed of particulate matter with a quantity of a non-ionic resin to adsorb phenolic compounds contained in the mare urine material and separating the resin laden with the phenolic compounds from a first residual mare urine material; (b) contacting the first residual mare urine material with a quantity of weak cationic resin to adsorb the major conjugated estrogen components contained in the mare urine material and separating the resin laden with the major conjugated estrogen components from a second residual mare urine material; (c) contacting the second residual mare urine material with a quantity of a non-ionic resin to adsorb the minor conjugated estrogen components contained in the mare urine material and separating the resin laden with the minor conjugated estrogen components from a third residual urine material; (d) contacting the resin laden with the major conjugated estrogen components with a sufficient quantity of an elution liquid selected from the group consisting of isopropyl alcohol and methanol to desorb the adsorbed major conjugated estrogen components and recovering an eluant containing crude major conjugated estrogen components; and (e) contacting the resin laden with the minor conjugated estrogen components with a sufficient quantity of an elution liquid selected from the group consisting of isopropyl alcohol and methanol to desorb the adsorbed minor conjugated estrogen components and recovering an eluant containing crude minor conjugated estrogen components.