Patent ID: 6124478
Filing Date: 2000-09-26
Classification: C07D,C07F,G01N,Y02P

Abstract:
A kit for the generation of light in an optically detectable assay, comprising: (a) a 1,2-dioxetane of the formula: ##STR50## wherein n=0-19, wherein Z is an enzyme cleavable group which, when cleaved, yields an anion selected from the group consisting of an oxygen anion, a sulfur anion, an amine anion, a nitrogen anion and an amide anion, andQ is a moiety selected from the group consisting of trifluoromethyl, fluoro, chloro, cyano, nitrogen, --COOCH.sub.2 CH.sub.3, --COCH.sub.3, SO.sub.2 NH Ar, 2-(5-fluoresceinyl)-ethoxy, phenoxy, hydroxymethyl, hydroxyethyl, 3,5-diethoxyphenyl, benzoxazole, benzothiazole, benzimidazole and benzotriazole, and(b) an enzyme which, when brought into contact with said 1,2-dioxetane, cleaves said moiety Z; andwherein Ar is phenyl or naphthyl.