Patent ID: 8916340
Filing Date: 2014-12-23
Classification: A61K,A61P,C07K,C12N,C12P

Abstract:
1. A method for producing an isolated or purified complex of an MHC class II restricted peptide from a polypeptide of interest, comprising: (a) optionally, denaturing the polypeptide of interest to produce a denatured polypeptide; (b) incubating the polypeptide or optionally denatured polypeptide in the presence of  such that the polypeptide or optionally denatured polypeptide binds to the peptide binding groove of the MHC class II protein, forming a complex with said class II protein; (c) proteolytically digesting exposed regions of said polypeptide or optionally denatured polypeptide so that a peptide of about 10 to about 26 amino acid residues remains bound to the peptide binding groove of said MHC class II protein, thereby producing said complex; and (d) further isolating or purifying said complex, thereby producing said isolated or purified complex of said MHC class II restricted peptide from said polypeptide of interest.