Patent ID: 6203998
Filing Date: 2001-03-20
Classification: C07K,C12Q,G01N

Abstract:
A method of screening a compound for binding to a human D4 dopamine receptor, the method comprising the following steps:(a) transforming a host cell with a recombinant expression construct encoding a human D4 dopamine receptor having an amino acid sequence that is the amino acid sequence of the human D4 dopamine receptor allele D4.4 from amino acid 1 to amino acid 248 (SEQ ID No.: 20), covalently linked at the carboxyl terminus of amino acid 248 to the amino terminus of a repeated amino acid sequence, the carboxyl terminus of the repeated amino acid sequence being covalently linked to the amino terminus of the amino acid sequence of the human D4 dopamine receptor allele D4.4 from amino acid 312 to amino acid 419 (SEQ ID No.: 20), wherein the amino acid sequence of the repeated sequence comprises from 3 to 8 copies of the amino acid sequence:(Pro/Ala).Ala.Pro.(Arg/Gly).Leu.Pro.(Gln/Arg/Pro).(Asp/Gly).Pro.Cys.Gly. (Pro/Ser).(Asp/Asn).Cys.Ala.Pro (SEQ ID No.:24),wherein the cells of the transformed cell culture express the receptor; and(b) assaying the transformed cell with the compound to determine whether the compound binds to the human D4 dopamine receptor.