Patent ID: 9109244
Filing Date: 2015-08-18
Classification: C12P,Y02P

Abstract:
1. A process for the production of at least one microbial metabolite having at least 3 carbon atoms, or having at least 2 carbon atoms and at least 1 nitrogen atom by means of sugar-based microbial fermentation, comprising: a) the preparation of a sugar-containing liquid hydrolyzate with a monosaccharide content of more than 20% by weight from a starch feedstock, the sugar-containing liquid hydrolyzate also comprising at least 20% by weight of the total non-starchy solid constituents of the starch feedstock; b) the fermentation for the production of the at least one metabolite, which comprises culturing, by using the sugar-containing liquid hydrolyzate, a microorganism strain which produces the at least one metabolite, where a fermentation liquor is obtained which contains the at least one metabolite; and c) depletion or isolation of at least one metabolite from the fermentation liquor, where the sugar containing liquid hydrolyzate is obtained in step a) by: a1) milling the starch feedstock, Which is selected from cereal kernels, by dry-milling process; and a2) liquefying the millbase in an aqueous liquid in the presence of at least one starch-liquefying enzyme, followed by saccharification using at least one saccharifying enzyme, to obtain a sugar-containing liquid hydrolyzate having a monosaccharide content of more than 20% by weight and also comprising at least 20% by weight of the total non-starchy solid constituents of the starch, where at least 40% of the millbase is added continuously or batchwise to the aqueous liquid in the course of the liquefaction step where the liquefaction is carried out at least in part above the gelling temperature of the starch employed and where at least 25% by weight of the total amount of the millbase added during the liquefaction are added at a temperature above the gelling temperature of the starch present in the millbase; and where the at least one metabolite produced is selected from the group consisting of organic mono-, di- and tricarboxylic acids which optionally have hydroxyl groups attached to them and which have 3 to 10 carbon atoms, among proteinogenic and nonproteinogenic amino acids, purine bases, pyrimidine bases, nucleosides, nucleotides, lipids, saturated and unsaturated fatty acids, dials having 3 to 10 carbon atoms, higher-functionality alcohols having 3 or more hydroxyl groups, longer-chain alcohols having at least 4 carbon atoms, carbohydrates, aromatic compounds, vitamins, provitamins, cofactors, nutraceuticals, proteins, carotenoids, ketones having 3 to 10 carbon atoms, lactones, biopolymers, and cyclodextrins.