Patent ID: 8119360
Filing Date: 2012-02-21
Classification: C12Q,G01N

Abstract:
1. A method for quantitatively determining cholesterol in remnant-like particles in a sample, which comprises: obtaining an aqueous medium containing the sample, a phospholipid-hydrolyzing enzyme, a polyoxyethylene-polyoxybutylene copolymer, and one or more surfactants selected from the group consisting of a polyoxyethylene-polyoxypropylene alkylaryl ether, a polyoxyethylene-polyoxypropylene alkyl ether and a polyoxyethylene-polyoxypropylene short-chain branched alkyl ether; reacting a combination of cholesterol esterase and cholesterol oxidase or a combination of cholesterol esterase, oxidized coenzyme and cholesterol dehydrogenase with cholesterol in remnant-like particles in the sample in the aqueous medium to form hydrogen peroxide or reduced coenzyme; and determining an amount of the formed hydrogen peroxide or reduced coenzyme.