Patent ID: 8574915
Filing Date: 2013-11-05
Classification: G01N,Y10T

Abstract:
1. A method for determining the amount of thyroglobulin in a test sample, comprising: (a) digesting thyroglobulin from the test sample to form peptide T129; (b) ionizing the peptide T129 to generate a multiply charged ion detectable by mass spectrometry; wherein the ion has a mass/charge ratio (m/z) of 636.4±0.5; and (c) detecting the amount of one or more T129 fragment ions each having a m/z selected from the group consisting of 541.3±0.5, 612.3±0.5, 636.4±0.5, 726.4±0.5, 797.4±0.5, 912.4±0.5, and 1059.5±0.5 by mass spectrometry; wherein the amount of the ions detected is related to the amount of the thyroglobulin in the test sample.