Patent ID: 6156177
Filing Date: 2000-12-05
Classification: G01N

Abstract:
A method for analyzing samples comprising the steps of:introducing samples labeled with fluorophores into a first end of each of a plurality of electrophoresis lanes including a separation medium, a second end of each of the electrophoresis lanes being disposed in an optical cell filled with a buffer solution;applying an electric field to the electrophoresis lanes to separate the samples labeled with the fluorophores and cause the separated samples labeled with the fluorophores to migrate through the electrophoresis lanes into the buffer solution in the optical cell;exciting, with light from a light source, the fluorophores labeling the separated samples in the buffer solution in the optical cell to emit fluorescence in the buffer solution in the optical cell, the light source being selected from the group consisting ofa He--Ne laser which emits light having a wavelength of 594 nm,a He--Ne laser which emits light having a wavelength of 632.8 nm,a YAG laser which emits light having a wavelength of 532 nm, anda semiconductor laser which emits light having a wavelength of 670 nm; anddetecting, with a photodetector, the fluorescence emitted by the fluorophores labeling the separated samples in the buffer solution in the optical cell.