Patent ID: 8273311
Filing Date: 2012-09-25
Classification: B03C

Abstract:
1. A biological particle separation method for separating wanted positive particles from particle mixture liquid containing mixture of biological particles, comprising: a first step of combining magnetic reactive material only with positive particles among biological particles contained in the particle mixture liquid; a second step of injecting liquid-drops of the particle mixture liquid into a vessel; a third step of forming magnetic field inside of particle mixture liquid by applying magnetic field above the particle mixture liquid contained in the vessel; and a fourth step of collecting positive particles being moved upward toward a direction of the magnetic force by the magnetic field and negative particles being moved downward toward a direction of gravity collected respectively, wherein the method uses a biological particle separation apparatus comprising an upper panel containing a vessel which receives liquid-drops, a lower panel containing a vessel which receives liquid-drops, wherein the lower panel is situated below the upper panel; and a magnetic field application part applying magnetic field, which is situated above the upper panel, wherein the upper panel is situated so that an entrance of its vessel is directed downward, the lower panel is situated so that an entrance of its vessel is directed upward, and the entrances of the vessels in the upper panel and the lower panel are situated to the direction of facing each other mutually, wherein the method further comprises, after injecting the liquid-drops of particle mixture liquid into the vessel of the upper panel or the lower panel, having liquid-drops of the particle mixture liquid contacted to the inside of the vessel of the upper panel or lower panel by moving the upper panel and the lower panel in a vertical direction to reduce a gap between the vessel of the upper panel and the vessel of the lower panel, and having the upper panel and lower panel returned to original positions, and wherein, in the second step, the vessel is any one of the vessel of the upper panel and the vessel of the lower panel.