Patent ID: 6730488
Filing Date: 2004-05-04
Classification: C07K,G01N

Abstract:
An immunoassay for detecting or determining the amount of dihydropyrimidine dehydrogenase in a biological sample which comprises:contacting a monoclonal antibody that specifically binds to dihydropyrimidine dehydrogenase with a biological sample suspected of having dihydropyrimidine dehydrogenase therein, wherein said monoclonal antibody is produced by a hybridoma cell line selected from the group consisting of hybridoma cell lines 3A5-6-1 (FERM BP-6015), 4B9-12-1 (FERM BP-6016) and 2E2-B3-1-3 (FERM BP-6014); allowing said monoclonal antibody to specifically bind to any dihydropyrimidine dehydrogenase present in the sample to form an immunoconjugate; and, qualitatively or quantitatively detecting the presence or absence of the immunoconjugate wherein the presence of the immunoconjugate is indicative of the presence of dihydropyrimidine dehydrogenase in the biological sample.