Patent ID: 6010880
Filing Date: 2000-01-04
Classification: A61P,C04B,C07K

Abstract:
An isolated protein that inhibits plasmin comprising a Kunitz domain having the formula:Xaa1-Xaa2-Xaa3-Xaa4-Cys-Xaa6-Xaa7-Xaa8-Xaa9-Xaa10- Xaa11-Gly-Xaa13-Cys-Xaa15-Xaa16-Xaa17-Xaa18-Xaa19- Arg-Xaa21-Xaa22-Xaa23-Xaa24-Xaa25-Xaa26-Xaa27- Xaa28-Xaa29-Cys-Xaa31-Xaa32-Phe-Xaa34-Xaa35-Xaa36- Gly-Cys-Xaa39-Xaa40-Xaa41-Xaa42-Xaa43-Xaa44-Xaa45- Xaa46-Xaa47-Xaa48-Xaa49-Xaa50-Cys-Xaa52-Xaa53- Xaa54-Cys-Xaa56-Xaa57-Xaa58, whereinXaa1, Xaa2, Xaa3, Xaa4, Xaa56, Xaa57 or Xaa58 may be absent;Xaa10 is selected from Asp, Glu, Tyr and Gln;Xaa11 is selected from Thr, Ala, Ser, Val and Asp;Xaa13 is selected from Pro, Leu and Ala;Xaa15 is selected from Lys and Arg;Xaa16 is selected from Ala and Gly;Xaa17 is selected from Arg, Lys and Ser;Xaa18 is selected from Phe and Ile;Xaa19 is selected from Glu, Gln, Asp, Pro, Gly, Ser and Ile;Xaa21 is selected from Phe, Tyr and Trp;Xaa22 is selected from Tyr and Phe;Xaa23 is selected from Tyr or Phe;Xaa31 is selected from Asp, Glu, Thr, Val, Gln and Ala;Xaa32 is selected from Thr, Ala, Glu, Pro and Gln;Xaa34 is selected from Val, Ile, Thr, Leu, Phe, Tyr, His, Asp, Ala and Ser;Xaa35 is selected from Tyr and Trp;Xaa36 is selected from Gly and Ser;Xaa39 is selected from Glu, Gly, Asp, Arg, Ala, Gln, Leu, Lys and Met;Xaa40 is selected from Gly and Ala;Xaa43 is selected from Asn and Gly; andXaa45 is selected from Phe and Tyr.