Patent ID: 8299224
Filing Date: 2012-10-30
Classification: C07H

Abstract:
1. A method for purifying Rebaudioside D from Stevia extract comprising the steps of: a. providing an extract of b. dissolving the extract in a first aqueous solution of organic solvent to result in a first mixture of steviol glycosides, wherein the organic solvent is selected from the group consisting of methanol, ethanol, l-propanol, isopropanol, and a mixture thereof, and the organic solvent is 75-99 vol. %; c. inducing crystallization in the first mixture; d. filtering the mixture from step (c) to obtain a first precipitate and a first filtrate; e. dissolving the first precipitate in a second aqueous solution of organic solvent to result in a second mixture, wherein the organic solvent is selected from the group consisting of methanol, ethanol, l-propanol, isopropanol, and a mixture thereof, and the organic solvent is 70-80-vol. %; f. inducing crystallization in the second mixture; g. filtering the mixture from step (f) to obtain a second precipitate and a second filtrate; h. dissolving the second precipitate in a third aqueous solution of organic solvent to result in a third mixture, wherein the organic solvent is selected from the group consisting of methanol, ethanol, l-propanol, isopropanol, and a mixture thereof, and the organic solvent is 10-80 vol. %; i. inducing crystallization in the third mixture; and j. filtering the mixture from step (i) to obtain a third precipitate and a third filtrate;