Patent ID: 7985547
Filing Date: 2011-07-26
Classification: C12Q

Abstract:
1. A method for identifying sequences in a polynucleotide, comprising: (a) cleaving the polynucleotide with a first type-IIS endonuclease to obtain fragments; (b) ligating a first adapter sequence to the fragments generated in step (a) to obtain first adapter-ligated fragments, the first adapter comprising a recognition site for a second type-IIS endonuclease; (c) cleaving the adapter-ligated fragments with the second type-IIS endonuclease; (d) ligating a second adapter sequence to the fragments generated in step (c) to obtain second adapter-ligated fragments; and (e) identifying sequences in the polynucleotide by determining the sequence of a plurality of nucleotides captured between the first adapter sequence and the second adapter sequence in the second adapter-ligated fragments.