Patent ID: 7553619
Filing Date: 2009-06-30
Classification: C12Q,G01N

Abstract:
1. A method for detecting one or more analytes, the method comprising (a) bringing into contact one or more analyte samples and one or more reporter binding molecules, wherein each reporter binding molecule comprises a specific binding molecule and an amplification target circle, wherein each specific binding molecule interacts with an analyte directly or indirectly, incubating the analyte samples and the reporter binding molecules under conditions that promote interaction of the specific binding molecules and analytes, and separating the specific binding molecules that interact with the analytes from the specific binding molecules that do not interact with the analytes, (b) decoupling the amplification target circles associated with the analytes from the specific binding molecules, (c) bringing into contact the decoupled amplification target circles and one or more rolling circle replication primers, wherein the amplification target circles each comprise a single-stranded, circular DNA molecule comprising a primer complement portion, wherein the primer complement portion is complementary to at least one of the rolling circle replication primers, and incubating the rolling circle replication primers and amplification target circles under conditions that promote hybridization between the amplification target circles and the rolling circle replication primers, (d) incubating the rolling circle replication primers and amplification target circles under conditions that promote replication of the amplification target circles, wherein replication of the amplification target circles results in the formation of tandem sequence DNA, wherein detection of tandem sequence DNA indicates the presence of the corresponding analytes.