Patent ID: 7189519
Filing Date: 2007-03-13
Classification: B01F,C07D,C40B,G01N,Y10T

Abstract:
1. A method for detecting ligand-receptor-GPCR complexes, comprising the steps of: (a) providing a sample suspension containing at least one set of G-protein beads, wherein said G-protein beads are epitope-recognizing beads having an epitope-containing heterotrimeric G-protein bound thereto; (b) mixing said sample suspension with at least one type of G-protein coupled receptor and a ligand wherein said receptor or said ligand contains a detectable label, to thereby form a mixed suspension containing a detectable ligand-receptor-G protein complex when said G protein forms said detectable complex with said receptor and said ligand; (c) detecting said ligand-receptor-G protein complexes in said mixed suspension by flow cytometry.