Patent ID: 6187994
Filing Date: 2001-02-13
Classification: C07K,C12N

Abstract:
A method to directly select transformed plant cells, said method comprising:a) transforming said plant cell with a transfer cassette, said transfer cassette comprises a first recombination site, a nucleotide sequence encoding a selectable marker gene not operably linked to a promoter, and a second recombination site, wherein said first and second sites are non-identical;b) wherein said plant genome comprises at least one expression cassette, said cassette comprises a promoter operably linked to a target site comprising a first non-identical recombination site a gene coding region and a second non-identical recombination site, wherein said first and second non-identical recombination site correspond to the flanking sites of said transfer cassette;c) providing a recombinase that recognizes and implements a recombination event between the first recombination site in the transfer cassette and the first recombination site in the target site, and a second recombination event between the second recombination site in the transfer cassette and the second recombination site in the target site; and,d) growing said plant cells on the appropriate selective agent to recover cells which have successfully undergone targeted integration of the transfer cassette at the target site leading to activation of expression of the selectable marker.