Patent ID: 6410725
Filing Date: 2002-06-25
Classification: C12N,C12Q

Abstract:
A method for extracting DNA from a biological sample dried on a solid support which comprises:(a) contacting the biological sample with a DNA extraction composition comprising: (1) 5% to 90% formamide; (2) 5 mM to 60 mM citrate; and (3) 1 mM to 300 mM buffer, pH 6.0 to 8.8; (b) incubating the mixture for 0.5 to 60 minutes at 4Â° to 60Â° C.; (c) incubating the mixture for 0.5 to. 60 minutes at 45Â° to 100Â° C.; and (d) isolating a supernatant containing extracted DNA.