Patent ID: 8030028
Filing Date: 2011-10-04
Classification: C12N,C12P

Abstract:
1. A method of producing a protein in a cell with a homoglutamine at a specified position, the method comprising: growing, in an appropriate medium, the cell, where the cell comprises a nucleic acid that comprises at least one selector codon and encodes a protein; and, providing the homoglutamine; wherein the cell further comprises: an orthogonal-tRNA (O-tRNA) that recognizes the selector codon; and, an orthogonal aminoacyl-tRNA synthetase (O-RS) that preferentially aminoacylates the O-tRNA with the homoglutamine, wherein the O-RS is at least 95% identical to PhAAD (SEQ ID NO: 28) and comprises an I41 and S268 mutation in said PhAAD (SEQ ID NO: 28); and wherein the O-tRNA comprises a CU(X) incorporating the homoglutamine into the specified position in response to the selector codon, thereby producing the protein.