Patent ID: 6472171
Filing Date: 2002-10-29
Classification: C07K,C12N

Abstract:
A method for making a mammalian collagen or procollagen in yeast, the method comprising the steps of:(a) incubating a yeast cell comprising: (i) a stable genetic construct comprising in opposite orientations, first and second mammalian collagen genes operably linked to a first single divergent heterologous promoter; (ii) a prolyl hydroxylase gene; (iii) a protein disulfide isomerase gene, wherein the prolyl hydroxylase and protein disulfide isomerase genes are expressed to produce polypeptides that are associated into active proteins during the incubating step, in a medium under conditions wherein the collagen genes are expressed as procollagen chains and the chains secreted in the form of triple helical procollagen; and (b) recovering the triple helical procollagen.