Patent ID: 8361713
Filing Date: 2013-01-29
Classification: B01J,B01L,C12Q,C40B,G01N

Abstract:
1. A method of sequencing multiple target polynucleotides in parallel using a sequencing system, comprising: providing a flow chamber comprising a plurality of ridges within the flow chamber running parallel to the axis of the flow chamber, the flow chamber comprising multiple microparticles immobilized between the ridges, each microparticle of the multiple microparticles including a target polynucleotide to be sequenced immobilized on the microparticle, the average distance between centers of adjacent microparticles being less than two microparticle diameters, and the ridges being of a height less than a diameter of a microparticle and of a length greater than a plurality of said diameters; providing an oligonucleotide through an inlet of the flow chamber; ligating the oligonucleotide to a strand hybridized to a polynucleotide of the plurality of target polynucleotides when the oligonucleotide is complementary to a single-stranded portion of the polynucleotide; obtaining data that provides information about the plurality of target polynucleotides based on the ligation of the oligonucleotides, the information produced being governed by at least a portion of a sequence of the plurality of target polynucleotides; and cleaving at least a portion of the oligonucleotide that was ligated.