Patent ID: 6046383
Filing Date: 2000-04-04
Classification: A01H

Abstract:
A method for producing cytoplasmic male sterile Brassica oleracea plants which are sterile at high and low temperature and which contain polima CMS cytoplasm, the method comprising the steps of:a) crossing Brassica campestris cultivar 87110 having ATCC Accession Number 97246 with Brassica oleracea cultivar 87101 and collecting and seeds resulting from said cross;b) regenerating said seeds into Brassica napus plants which contain a haploid set of chromosomes;c) doubling the chromosomes of the plants of step b to provide Brassica napus plants containing a diploid set of chromosomes;d) crossing Brassica campestris cultivar 87118 having ATCC Accession Number 97247 with Brassica oleracea cultivar 87101 and collecting the seeds resulting from said cross;e) regenerating said seeds of step d into Brassica napus plants which contain a haploid set of chromosomes;f) doubling the chromosomes of the plants of step e to provide Brassica napus plants containing a diploid set of chromosomes;g) crossing the plants of step f with Brassica napus cultivar 87102 which is male sterile and contains polima CMS cytoplasm and collecting the seeds resulting from said cross;h) regenerating said seeds of step g into Brassica napus plants containing the polima CMS cytoplasm;i) crossing the plants of step c with the plants of step h and collecting the seeds resulting from said cross;j) regenerating said seeds of step i into Brassica napus plants which are male sterile and contain the polima CMS cytoplasm;k) crossing the plants of step j with a Brassica oleracea cultivar and collecting the seeds resulting from said cross;l) removing the embryos from the seeds of step k and regenerating said embryos into F.sub.1 plants which are male sterile and contain the polima CMS cytoplasm;m) backcrossing the plants from step l with a Brassica oleracea cultivar and collecting the seeds resulting from said backcrossing;n) removing the embryos from the seeds of step m and regenerating said embryos into plants which are male sterile and contain the polima CMS cytoplasm;o) backcrossing the plants from step n with a Brassica oleracea and collecting the seeds resulting from said backcrossing; andp) regenerating said seeds of step o into Brassica oleracea plants which are male sterile at high and low temperatures and contain the polima CMS cytoplasm.