Patent ID: 6294323
Filing Date: 2001-09-25
Classification: C12Q

Abstract:
A method of producing at least one copy of a pair of complementary single stranded polynucleotide sequences, said method comprising:(a) forming in the presence of nucleoside triphosphates and template dependent polynucleotide polymerase along each of said complementary single stranded polynucleotide sequences, an extension of a single polynucleotide primer, said polynucleotide primer being comprised of at least a sequence of 16 nucleotides terminating at its 3' end in a 4 to 8 nucleotide sequence (S1) that is complementary with the 3' ends of both of said complementary single stranded polynucleotide sequences, wherein said polynucleotide primer has a specifically designed sequence of at least 8 nucleotides (S2) that is 5' of said S1, said S2 being 50 to 80% complementary to each of the nucleotide sequences contiguous with the 3' ends of said complementary single stranded polynucleotide sequences, and(b) dissociating said extended polynucleotide primer and said single stranded polynucleotides thereby producing said copy of said single stranded polynucleotide sequences.