Patent ID: 9012184
Filing Date: 2015-04-21
Classification: C12N,C12Q

Abstract:
1. A method of reducing representation of the ends of a primary polynucleotide sequence in a library of fragments generated from said primary polynucleotide sequence, the method comprising: a) performing an amplification reaction with a modified forward primer and a modified reverse primer to introduce a modification at or near each 5′ terminus of a double-stranded primary polynucleotide sequence, said modification configured to prevent fragments originating from the ends of the primary polynucleotide from ligating to an adapter, thereby forming a modified primary polynucleotide sequence; b) fragmenting the modified primary polynucleotide sequence such that the 5′ terminal fragment and the 3′ terminal fragment each comprise the modification; c) ligating an adapter to fragments generated in step b), thereby forming a library of fragments generated from said primary polynucleotide sequence.