Patent ID: 6645723
Filing Date: 2003-11-11
Classification: C07K,G01N

Abstract:
Method for determining growth hormone receptor activation by the use of GHR05 antibody, wherein the GHR05 antibody binds with a higher affinity to the active conformation of growth hormone receptor than it does to the inactive conformation of growth hormone receptor, the method comprising the steps of:(a) contacting the GHR05 antibody and the receptor to form a GHR05-receptor complex; (b) measuring the amount of the GHR05 antibody bound to the GHR05-receptor complex; (c) contacting a ligand to the GHR05-receptor complex to form a ligand-GHR05-receptor complex; (d) measuring the amount of the GHR05 antibody bound to the ligand-GHR05-receptor complex; and (e) comparing the amount of the GHR05 antibody bound in the presence of ligand to the amount of the GHR05 antibody bound in the absence of ligand, wherein growth hormone receptor activation is determined by an increase in the amount of the GHR05 antibody bound in the presence of ligand.