Patent ID: 9102980
Filing Date: 2015-08-11
Classification: C12N,C12Q

Abstract:
1. A method of sample analysis, comprising: a) annealing a reflex sequence in a polynucleotide strand in a sample with a complement of the reflex sequence present in the polynucleotide strand, wherein the polynucleotide strand comprises the following in a 5′ to 3′ orientation: b) extending from the 3′ end of the complement of the reflex sequence through the first domain to produce a double stranded region comprising a complement of the first domain at the 3′ end of the extended polynucleotide strand, thereby moving the first domain into proximity with the desired site; and c) amplifying the desired site from the polynucleotide strand of step b) using a first nucleic acid synthesis primer that anneals at the 3′ end of the complement of the first domain and a second nucleic acid synthesis primer that comprises a 3′ priming domain that is complementary to a site within the polynucleotide of interest.