Patent ID: 6632599
Filing Date: 2003-10-14
Classification: G01N,Y10S

Abstract:
A method for the simultaneous detection of the presence or amount of multiple cell-associated factors, which are associated with the same cell, said method comprising:1) providing a sample containing a cell comprising at least one cell-associated factor Fx and at least one cell-associated factor Fy; 2) selecting a receiver R and a transmitter T which enter into interaction with each other when in spatial proximity, and wherein the interaction directly or indirectly provides a signal;3) providing at least one stable receiver complex, Lx-R, comprising the receiver R, and at least one ligand Lx, wherein Lx has binding affinity for the at least one cell-associated factor Fx;4) providing at least one stable transmitter complex, Ly-T, comprising the transmitter T, and at least one ligand Ly, wherein Ly has binding affinity for the at least one cell-associated factor Fy;5) contacting the sample with the at least one stable receiver complex and the at least one stable transmitter complex, wherein, in the presence of the at least one cell-associated factor Fx and Fy, complexes Fx-Lx-R and Fy-Ly-T are formed and;6) detecting a signal provided by the interaction of T and R, wherein the detection of the signal correlates with the detection of the presence or amount of multiple cell-associated factors.