Patent ID: 6872870
Filing Date: 2005-03-29
Classification: C07K,C12N,C12Q

Abstract:
1. A method for the preparation of a plant, showing constitutive, inducible and/or organ specific expression of a specifically modified plant TPS gene, comprising the steps of: a) providing a TPS protein encoded by a TPS gene from a plant; b) designing a suitable modification to the plant TPS gene by aligning the plant gene with the TPS gene of yeast and establishing which part of the plant gene extends beyond the 5′ terminus of the yeast gene; c) deleting the complete part of the plant TPS gene extending beyond the 5′ terminus of the yeast gene, in order to achieve an increased trehalose-6-phosphate synthase activity; d) cloning the thus modified plant TPS gene into an expression vector under the control of a constitutive, inducible and/or organ-specific promoter; and e) transforming a plant cell or tissue with the thus obtained expression vector.