Patent ID: 7811793
Filing Date: 2010-10-12
Classification: A61K,C07K

Abstract:
1. A process for producing a purified refolded monomeric MP52, comprising a) expressing a monomeric MP52 in the form of an inclusion body in b) treating the recovered inclusion body with a denaturing buffer to obtain a solubilized monomeric MP52; c) treating the solubilized monomeric MP52 directly with a refolding solution to obtain a refolded monomeric MP52; and then d) subjecting the refolded monomeric MP52 to purification; wherein the monomeric MP52 contains an amino acid sequence as set forth in SEQ. ID. NO: 1 wherein X in SEQ. ID. NO:1 is not cysteine; and wherein the purified and refolded monomeric produced in step d) is biologically active; and wherein the denaturing buffer contains cysteine.