Patent ID: 8163523
Filing Date: 2012-04-24
Classification: A61K,A61P,C12N,C12Y

Abstract:
1. A cell culture-based method for producing influenza virus vaccine, comprising (A) infecting a Chinese Hamster Ovary cell (CHO) with a human influenza virus that binds to cell surface 2,6-linked sialic acids, and (B) isolating human influenza viruses produced from the CHO cell, and (C) formulating the isolated human influenza viruses into an influenza virus vaccine, wherein the CHO cell expresses a ST6GAL1 gene and comprises cell-surface bound 2,6-linked sialic acids, and wherein the susceptibility of the CHO cell to human influenza virus infection is greater than a CHO cell that does not express the ST6GAL 1 gene.