Patent ID: 6821766
Filing Date: 2004-11-23
Classification: C12N,C12Y

Abstract:
An isolated thermostable creatine amidinohydrolase that is isolated from E. coli JM109 (pUCE100B-40) (accession number FERM BP-6867), and which has the following physicochemical properties:(a) hydrolyzing 1 mol of creatine to give 1 mol of sarcosine and 1 mol of urea; (b) having a substrate specificity to creatine; (c) having an optimum pH of 7.0 to 8.0; (d) having a stable pH range of 4.0 to 11.0; (e) having the optimum temperature of around 45Â° C.; (f) being thermostable at 53Â° C.; (g) having a molecular weight of 92,000 Da (as determined by gel filtration).