Patent ID: 8658608
Filing Date: 2014-02-25
Classification: C12N

Abstract:
1. A recombinagenic or mutagenic composition comprising a single-stranded oligonucleotide that binds to a polypyrimidine:polypurine target motif in a double stranded nucleic acid molecule in a beta globin gene to form a triple-stranded nucleic acid molecule, wherein the polypyrimidine strand of the polypyrimidine:polypurine target motif comprises from nucleotide 837 to nucleotide 846 of SEQ ID NO:59 or nucleotide 774 to nucleotide 787 of SEQ ID NO:60, wherein the single-stranded oligonucleotide comprises a sequence substantially complementary to the polypurine strand of the polypyrimidine:polypurine target motif, and wherein the single stranded oligonucleotide comprises one or more chemically modified cytosine nucleotides substituted for one or more cytosine nucleotides and has increased triplex stability at neutral pH relative to the single-stranded oligonucleotide in the absence of the substituted cytosine nucleotides.