Patent ID: 9097729
Filing Date: 2015-08-04
Classification: G01N

Abstract:
1. A method of selecting a specific binding agent to an epidermal growth factor receptor (EGFr) polypeptide, wherein the specific binding agent binds to at least a portion of a panitumumab epitope on an EGFr polypeptide, comprising: a) contacting a first EGFr polypeptide with an agent, wherein the first EGFr polypeptide comprises an L2 domain; b) determining the affinity of the agent for the first EGFr polypeptide; c) contacting a second EGFr polypeptide with the agent, wherein the second EGFr polypeptide lacks an L2 domain; d) determining the affinity of the agent for the second EGFr polypeptide; and e) selecting the agent if the affinity for the first EGFr polypeptide is greater than the affinity for the second EGFr polypeptide.