Patent ID: 6379937
Filing Date: 2002-04-30
Classification: C12P

Abstract:
A process for the preparation of a mixture of 19 hydroxyeicosatetraenoic acid and 20 hydroxyeicosatetraenoic acid (19 HETE and 20 HETE), which comprises:(a) growing Candida apicola (ATCC 96134 and ATCC 24616) or Candida bombicola (ATCC 22214) in a growth medium comprising carbon and nitrogen sources supplemented with one or more Poly Unsaturated Fatty Acids (PUFA) for a period of 12 to 96 hrs to form a biomass, (b) separating the biomass from the medium (broth), (c) extracting the separated broth with an organic solvent, and (d) drying and hydrolyzing the extract to obtain 19 HETE and 20 HETE.