Patent ID: 7745168
Filing Date: 2010-06-29
Classification: C12Q,Y02A

Abstract:
1. A method for determining the presence or absence of a chitin-containing microorganism in a sample suspected of containing a chitin-containing microorganisms, the method comprising the steps of: providing a sample suspected of containing a chitin-containing microorganisms to be analyzed; treating the sample with 3-hydroxy-4((2-sulfo-4-((-4-sulfophenyl(azo)phenyl)azo) 2,7 naphthalenedisulfonic acid tetrasodium salt stain to stain the chitin of the chitin-containing microorganisms; treating the sample with a cationic carbocyanine 1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]naphto[1,2-d]thiazolium bromide dye, the cationic carbocyanine dye binding to the 3-hydroxy-4((2-sulfo-4-((-4-sulfophenyl(azo)phenyl)azo) 2,7 naphthalenedisulfonic acid tetrasodium salt stain to form a light-stable complex; applying three decolorizing solutions to the sample; rinsing the sample after each application of decolorizing solution; and examining the sample with a light microscope to identify the presence of chitin-containing microorganisms in the sample, wherein the stained chitin-containing microorganisms are observable in the light microscope.