Patent ID: 6828118
Filing Date: 2004-12-07
Classification: C12Q,G01N

Abstract:
A process for quantifying the ratio of type I to type II isozymes of human wild-type tPA (SEQ ID NO:4) or a variant or variants thereof in a sample comprising same, which process comprises:(a) incubating the sample with a protease capable of specifically cleaving the Arg275-Ile276 bond of said human wild-type tPA; (b) incubating the sample from step (a) with a denaturing agent and a reducing agent in amounts effective to reduce the disulfide bonds of said human wild-type tPA; (c) subjecting the sample to a reversed-phase high-performance liquid chromatography step; and (d) analyzing the elution profile from the chromatography step for the relative amounts of type I and type II isozymes present.