Patent ID: 7419834
Filing Date: 2008-09-02
Classification: G01N,Y10T

Abstract:
1. A method for detecting the presence of nitrosylated species in a biological sample comprising one or more amino acid-based species, the method comprising the steps of: (a) separating the amino acid-based species in the sample; (b) contacting the separated species with developing reagents comprising a fluorescence-developing agent that produces a detectable signal in the presence of nitric oxide; (c) contacting the separated species with a molecular species bearing a thiol moiety capable of transnitrosylation and a saturated solution of copper (I) chloride; (d) exposing the separated species to excitation radiation; and (e) detecting emitted fluorescence from the fluorescence-developing agent, wherein the emitted fluorescence indicates the presence of a nitrosylated species in the sample.