Patent ID: 7470507
Filing Date: 2008-12-30
Classification: C12Q,C40B,G01N

Abstract:
1. A method of identifying a region of a genome of a cell to which a protein of interest binds, comprising the steps of: a) crosslinking DNA binding protein in the cell to genomic DNA of the cell, thereby producing DNA binding protein crosslinked to genomic DNA; b) generating DNA fragments of the genomic DNA crosslinked to DNA binding protein in a) by sonication, thereby producing a mixture comprising DNA fragments to which DNA binding protein is bound; c) removing a DNA fragment to which the protein of interest is bound from a first portion of the mixture produced in b); d) separating the DNA fragment of c) from the protein of interest; e) labeling the DNA fragment of d) with a first fluorescent label by: f) labeling a second portion of the mixture produced in b) with a second fluorescent label by: g) combining the labeled DNA fragment of e) and the second sample of f) with a DNA microarray comprising sequences that detect intergenic regions, under conditions in which nucleic acid hybridization occurs; and h) comparing results obtained from said first fluorescent label to results obtained from said second fluorescent label to identify a sequence of g) to which the DNA fragment hybridizes, whereby the sequence identified in h) indicates the region of the genome in the cell to which the protein of interest binds.