Patent ID: 8530228
Filing Date: 2013-09-10
Classification: C12N,C12Q,G01N,Y10T

Abstract:
1. An integrated and versatile method for preparing a biological specimen, said method comprising: (a) contacting a protecting, lysis and inhibiting solution selected from the group consisting of lysis solution 1, lysis solution 2, lysis solution 3, and lysis solution 4 to a biological specimen; (b) forming a homogenate or a lysate comprising large RNA/mRNA/ccfRNA, DNA/ccfDNA, small RNA/miRNA/ccfmiRNA and protein; (c) sequentially performing a differential centrifugation process and a differential precipitation process on the lysate without toxic phenol/chloroform extraction to separate the DNA/ccfDNA, large RNA/mRNA/ccfDNA, small RNA/miRNA/ccfmiRNA and Protein; wherein the protecting, lysis and inhibiting solution inhibits endogenous degrading enzymes in the biological specimen.