Patent ID: 6063583
Filing Date: 2000-05-16
Classification: C07K,C12Q

Abstract:
A method to identify compounds which disrupt complex comprising cyclic AMP response element binding protein (CREB) and CREB binding protein (CBP), said method comprising:(a) contacting a modified host cell with a test compound, wherein said modified host cell comprises:a first fusion protein comprising a GAL4 DNA binding domain, operatively associated with the kinase-inducible domain (KID) of CREB,a second fusion protein comprising an activation domain, operatively associated with the CREB binding domain (KIX) of CBP, anda reporter construct comprising a GAL4 response element operatively linked to a reporter gene; and(b) selecting those test compounds which cause reduced expression of the reporter gene product, wherein said compounds are identified as disrupting complex comprising CREB and CBP.