Patent ID: 6177559
Filing Date: 2001-01-23
Classification: B01D,B01J,C12N,C12Q

Abstract:
A batch process for obtaining polynucleotide fragments having a selected size from a mixture of polynucleotide fragments comprising the steps ofa) applying a solution of said mixture of polynucleotide fragments and a counterion agent to a binding medium having a hydrophobic surface;b) contacting the binding medium with a first stripping solvent and counterion agent, the first stripping solvent having a concentration of organic component sufficient to release from the binding medium all polynucleotide fragments having a size smaller than the selected size, and removing the first stripping solvent from the binding medium; andc) contacting the binding medium with a second stripping solvent having a concentration of organic component sufficient to release from the binding medium the polynucleotide fragments having the selected size, and removing the second stripping solvent from the binding medium.