Patent ID: 7816092
Filing Date: 2010-10-19
Classification: G01N

Abstract:
1. A method for performing a rapid cell-based enzyme-linked immunosorbent assay (C-ELISA), comprising the steps of: (a) lysing cells in a quick lysis and coating buffer, wherein the quick lysis and coating buffer comprises water and a metal hydroxide selected from the group consisting of sodium hydroxide, potassium hydroxide and rubidium hydroxide; (b) coating a solid phase with cellular proteins released in situ in the quick lysis and coating buffer; (c) blocking the solid phase with a blocking reagent dissolved in a quick blocking buffer, wherein the quick blocking buffer comprises water and a metal hydroxide selected from the group consisting of potassium hydroxide, sodium hydroxide and rubidium hydroxide; (d) (i) incubating the solid phase with a primary detection agent in solution;