Patent ID: 8153441
Filing Date: 2012-04-10
Classification: G01N,H01J,Y10T

Abstract:
1. A method for analyzing fragmented structures of [ 123 I]ADAM, comprising: providing a high-performance liquid chromatography tandem mass spectrometer (HPLC-MS/MS); providing [ performing a Q1 scan on the parent ion by using a mass spectrometer in the HPLC-MS/MS, and fragmenting the parent ion into product ions to find optimum parameters of declustering potential (DP), entrance potential (EP), collision energy (CE), and collision cell exit potential (CXP) when the following m/z of the MRM transition product ions are obtained: m/z 385.0→m/z 340.0, m/z 385.0→m/z 212.5, m/z 385.0→m/z 196.5, m/z 385.0→m/z 184.5, m/z 385.0→m/z 180.5, m/z 385.0→m/z 165.6, m/z 385.0→m/z 152.5; providing the HPLC-MS/MS and setting: eluent, methanol/acetonitrile/ammonium acetate solution (10 mM, pH 7.0), 79.68:19.92:0.40 (v/v/v); and flow rate, 0.4 mL/min; performing a multiple reaction monitoring (MRM) chromatography on [ deducing a fragmentation pathway of the parent molecule [