Patent ID: 7807435
Filing Date: 2010-10-05
Classification: C07K

Abstract:
1. A method for the purification of alpha-1 proteinase inhibitor (a1PI) from a protein precipitate, comprising the steps of a. providing a frozen protein precipitate comprising a1PI, wherein the frozen protein precipitate comprises at least one Cohn fraction selected from the group consisting of Cohn fraction IV b. thawing the protein precipitate at an ambient temperature of 2-25° C. until the protein precipitate has reached ambient temperature, without adding a suspending solvent, c. maintaining the thawed protein precipitate for at least 15 h (hours) at an ambient temperature of 2-25° C., and d. suspending the thawed, incubated precipitate of step c in a wash solution, and washing the protein precipitate; and e. extracting and purifying a1PI from the thawed, washed protein precipitate of step d.