Patent ID: 7985840
Filing Date: 2011-07-26
Classification: C07K

Abstract:
1. A library comprising a plurality of distinct polypeptides each comprising a heavy chain variable domain and a light chain variable domain, wherein each heavy chain variable domain comprises a variant CDRH3 region that comprises an amino acid sequence: (X wherein X X X X X X a variant CDRH1 and a variant CDRH2, wherein each of the variant CDRH1 and the variant CDRH2 has a variant amino acid at all solvent accessible and highly diverse amino acid positions, wherein CDRH1 comprises a variant amino acid at amino acid positions 28, 30, 31, 32 and 33; and CDRH2 comprises a variant amino acid at amino acid positions 50, 52, 53, 54, 56 and 58, the amino acid position numbering corresponding to the Kabat numbering system; and wherein each variant amino acid is encoded by a nonrandom codon set, and wherein at least 70% of the amino acids encoded by the nonrandom codon set are target amino acids for each of those positions in known antibody variable domains and 30% or less of the amino acids encoded by the nonrandom codon set are nontarget amino acids.