Patent ID: 7146277
Filing Date: 2006-12-05
Classification: G01N,G01R,Y10S

Abstract:
1. A method for improving the binding affinity of a ligand for a biological target comprising: a) preparing first NMR spectra of a first complex comprising the biological target and a paramagnetically labeled derivative of a first ligand to define a paramagnetic zone; b) preparing second NMR spectra of a second complex comprising the biological target and a second ligand; c) analyzing the spectra to determine whether the second ligand binds to the biological target within the paramagnetic zone of the paramagnetically labeled derivative; d) deducing a relative three-dimensional orientation of the first and second ligands when bound to the biological target; e) deducing a distance of separation of the first and second ligands when bound to the biological target; and f) selecting or preparing a compound that contains the first and second ligands substantially in the relative orientation and distance.