Patent ID: 6207807
Filing Date: 2001-03-27
Classification: C07K,G01N

Abstract:
A method for the separation and purification of immunoglobulins which comprises:(i) immobilizing on an affinity chromatography support a compound which non covalently binds to at least one immunoglobulin,(ii) packing said affinity chromatography support in a chromatographic column,(iii) equilibrating said column with a buffer capable of promoting an interaction between immunoglobulin and the immobilized compound,(iv) loading said column with a fluid comprising at least one immunoglobulin,(v) washing said column with at least one liquid capable of eluting the impurities without interfering with the interaction between immunoglobulin and the immobilized compound,(vi) eluting said immunoglobulin previously adsorbed on the column with a dissociating eluent,whereinsaid compound which non covalently binds to at least one immunoglobulin is a compound of formula (I)(H.sub.2 N--X.sub.1 --Thr--X.sub.2 --CO).sub.n --R (1)whereX.sub.1 and X.sub.2, different from one another, are an amino acid residue of arginine or tyrosine in configuration L or D, wherein the hydroxy group of threonine and tyrosine and the guanidine moiety of arginine is optionally protected by a compound conventionally used in peptide chemistry for protecting the hydroxy group and the guanidine moiety, respectively, n is 1,2, 3 or 4, andR, when n is 2,3 or 4, is a group suitable for forming a dimer, trimer or tetramer, and when n is 1,R is a single amino acid residue, or a peptide chain comprising 2 to 7 amino acid residues.