Patent ID: 6215042
Filing Date: 2001-04-10
Classification: C12N

Abstract:
A composition comprising nucleic acid molecules comprising an isolated DNA sequence encoding a plant branching enzyme from potato, said enzyme participating in the formation of .alpha.-1,6 linkages of glucose monomers, wherein the plant branching enzyme sequence comprises the following sequence, identified as SEQ ID NO: 1:10 20 30 40 50 60 1 TCAGGAGCGGTCTTGGGATATTTCTTCCACCCCAAAATCAAGAGTTAGAAAAGATGAAAG 51 GATGAAGCACAGTTCAGCTATTTCCGCTGTTTTGACCGATGACAATTCGACAATGGCACC 121 CCTAGAGGAAGATGTCAACACTGAAAATATTGGCCTCCTAAATTTGGATCCAACTTTGGA 181 ACCTTATCTAGATCACTTCAGACACAGAATGAAGAGATATGTGGATCAGAAAATGCTCAT 241 TGAAAAATATGAGGGACCCCTTGAGGAATTTGCTCAAGGTTATTTAAAATTTGGATTCAA 301 CAGGGAAGATGGTTGCATAGTCTATCGTGAATGGGCTCCTGCTGCTCAGGAAGCAGAAGT 361 TATTGGCGATTTCAATGGTAGGAACGGTTCTAACCACATGATGGAGAAGGACCAGTTTGG 541 AAAGTATGCCACTGCAGACGCCACAAAGTTTGCAGCACCATATGATGGTGTCTACTGGGA 601 CCCACCACCTTCAGAAAGGTACCACTTCAAATACCCTCGCCCTCCCAAACCCCGAGCCCC 661 ACGAATCTACGAAGCACATGTCGGCATGAGCAGCTCTGAGCCACGTGTAAATTCGTATCG 721 TGAGTTTGCAGATGATGTTTTACCTCGGATTAAGGCAAATAACTATAATACTGTCCAGTT 781 GATGGCCATAATGGAACATTCTTACTATGGATCATTTGGATATCATGTTACAAACTTTTT 841 TGCTGTGAGCAATAGATATGGAAACCCGGAGGACCTAAAGTATCTGATAGATAAAGCACA 901 TAGCTTGGGTTTACAGGTTCTGGTGGATGTAGTTCACAGTCATGCAAGCAATAATGTCAC 961 TGATGGCCTCAATGGCTTTGATATTGGCCAAGGTTCTCAAGAATCCTACTTTCATGCTGG 1021 AGAGCGAGGGTACCATAAGTTGTGGGATAGCAGGCTGTTCAACTATGCCAATTGGGAGGT 1081 TCTTCGTTTCCTTCTTTCCAACTTGAGGTGGTGGCTAGAAGAGTATAACTTTGACGGATT 1141 TCGATTTGATGGAATAACTTCTATGCTGTATGTTCATCATGGAATCAATATGGGATTTAC 1201 AGGAAACTATAATGAGTATTTCAGCGAGGCTACAGATGTTGATGCTGTGGTCTATTTAAT 1261 GTTGGCCAATAATCTGATTCACAAGATTTTCCCAGACGCAACTGTTATTGCCGAAGATGT 1321 TTCTGGTATGCCGGGCCTTAGCCGGCCTGTTTCTGAGGGAGGAATTGGTTTTGATTACCG 1381 CCTGGCAATGGCAATCCCAGATAAGTGGATAGATTATTTAAAGAATAAGAATGATGAAGA 1441 TTGGTCCATGAAGGAAGTAACATCGAGTTTGACAAATAGGAGATATACAGAGAAGTGTAT 1501 AGCATATGCGGAGAGCCATGATCAGTCTATTGTCGGTGACAAGACCATTGCATTTCTCCT 1561 AATGAACAAAGAGATGTATTCTGGCATGTCTTGCTTGACAGATGCTTCTCCTGTTGTTGA 1621 TGCAGGAATTGCGCTTGACAAGATGATCCATTTTTTTCACAATGGCCTTGGGAGGAGAGG 1681 GGTACCTCAATTTCATGGGTAACGAGTTTGGCCATCCTGAGTGGATTGACTTCCCTAGTG 1741 AGGGCAATAATTGGAGTTATGACAAATGTAGACGCCAGTGGAACCTCGCAGATAGCGAAC 1801 ACTTGAGATACAAGTTTATGAATGCATTTGATAGAGCTATGAATTCGCTCGATGAAAAGT 1861 TCTCATTCCTCGCATCAGGAAAACAGATAGTAAGCAGCATGGATGATGATAATAAGGTTG 1921 TTGTGTTTGAACGTGGTGACCTGGTATTTGTATTCAACTTCCACCCAAATAACACATACG 1981 AAGGGTATAAAGTTGGATGTGACTTGCCAGGGAAGTACAGAGTTGCACTGGACAGTGATG 2041 CTTGGGAATTTGGTGGCCATGGAAGAGCTGGTCATGATGTTGACCATTTCACATCACCAG 2101 AAGGAATACCTGGAGTTCCAGAAACAAATTTCAATGGTCGTCCAAATTCCTTCAAAGTGC 2161 TGTCTCCTGCGCGAACATGTGTGGCTTATTACAGAGTTGATGAACGCATGTCATAAACTG 2221 AAGATTACCAGACAGACATTTGTAGTGAGCTACTACCAACAGCCAATATCGAGGAAAGTG 2281 ACGAGAAACTTAAAGATTCATCATCTACAAATATCAGTACATCATCTACAAAAAATGCTT 2341 ATTACAGAGTTGATGAACGCATGTCAGAAGCTGAAGATTACCAGACAGACATTTGTAGTG 2401 AGCTACTACTACCAACAGCCAATATCGAGGAGAGTGACGAGAAACTTGATGATTCATTAT 2461 CTACAAATATCAGTAACATTGGTCAGACTGTTGTAGTTTCTGTTGAGGAGAGAGACAAGG 2521 AACTTAAAGATTCACCATCTGTAAGCATCATTAGTGATGCTGTTCCAGCTGAATGGGCTG 2581 ATTCGGATGCAAACGTCTGGGGTGAGGACTAGTCAGATGATTGATCGATCCTTCTACGTT 2641 GGTGATCTCGGTCCGTGCATGATGTCTTCAGGGTGGTAGCATTGACTGATTGCATCATAG 2701 TTTTTTTTTTTTTTTTTAAGTATTTCCTCTATGCATATTATTAGCATCCAATAAATTTAC 2761 TGGTTGTTGTACATAGAAAAAGTGCATTTGCATGTATGTGTTTCTCTGAAATTTTCCCCA 2821 GTTTTGGTGCTTTGCCTTTGGAGCCAAGTCTCTATATGTAATAAGAAAACTAAGAACAAT 2881 CACATATATAAAATGTTAGTAGATTACCAor a fragment or modified sequence thereof, which upon expression a plant leads to the formation of a protein participating in formation of .alpha.-1,6 linkages of glucose monomers.