Patent ID: 6113912
Filing Date: 2000-09-05
Classification: A61K,A61P,C07K,C12N,Y02A

Abstract:
A hepatitis A virus adapted to growth in MRC-5 cells, said virus having a genome whose cDNA sequence corresponds to the cDNA sequence of HAV HM-175, Pass 35 except for nucleotide positions 591 and 687 which have guanines as bases, nucleotide position 646 which has an adenine as a base and nucleotide position 669 which has a thymine as a base; and optionally, at least one additional nucleotide selected from the group consisting of:(a) T at position 2750;(b) A at position 3027;(c) A at position 3196(d) G at position 3934;(e) T at position 4418;(f) G at position 4563;(g) T at position 4643;(h) G at position 5145;(i) T at position 5745;(j) C at position 6908;(k) T at position 7032;(l) T at position 7255;wherein the nucleotide numbers shown above are those assigned to positions of the wild-type HM-175 sequence.