Patent ID: 6353150
Filing Date: 2002-03-05
Classification: A01K,C07K

Abstract:
A chimeric mouse comprising a stable bone marrow graft of human hematopoietic cells, wherein said graft differentiates into multiple lineages of mature human cells and wherein at least 30% of the hematopoietic cells in said bone marrow are of human origin, and wherein said graft is transplanted by a process comprising:a. sublethally irradiating an immunodeficient mouse, wherein the immunodeficient mouse lacks a population of functional T cells and B cells; b. infusing from about 106 to about 109 human hematopoietic cells into said immunodeficient mouse; and c. administering an effective amount of human mast cell growth factor (MGF) and a human granulocyte macrophage colony stimulating factor/Interleukin-3 fusion protein (GM-CSF/IL-3 FP) to promote engraftment of the human hematopoietic cells.