Patent ID: 7745129
Filing Date: 2010-06-29
Classification: C12Q

Abstract:
1. A method for determining base sequence data from a sample molecule of nucleic acid, comprising: (a) providing said sample molecule, (b) providing a substrate having a top surface, (c) providing a readout device, (d) extending said sample molecule in a first direction along said top surface, (e) attaching said sample molecule to said top surface at a plurality of locations along said sample molecule, (f) processing said sample molecule to form fragments attached to said top surface, said fragments comprising portions of said sample molecule and/or oligomers complementary to portions of said sample molecule, and said fragments having an original ordering along said first direction, (g) elongating said substrate substantially along said first direction such that said fragments are spatially separated and said ordering is maintained, (h) processing said fragments to produce signals correlated with the sequence of bases along each of said fragments, (i) using said readout device to detect and make a record of said signals and spatial ordering of said signals along said first direction, (j) analyzing said record to determine said base sequence data for said sample molecule, whereby said fragments are processed in parallel and said base sequence data of said sample molecule is constructed from a plurality of shorter sequences.