Patent ID: 8431356
Filing Date: 2013-04-30
Classification: G01N

Abstract:
1. A method for identifying a compound that modulates Sarco/Endoplasmic Reticulum Calcium ATPase (SERCA), comprising: (a) providing, in the presence of one or more lipids, SERCA labeled with a first chromophore at a first position and a second chromophore at a second position, wherein the second position is different from the first position, wherein the first and the second chromophores can be used for Fluorescence Resonance Energy Transfer (FRET), and wherein the method is suitable for high-throughput screening (HTS); (b) exciting either the first or the second chromophore; and (c) measuring FRET between the chromophores; wherein a difference between FRET in the presence of a test compound and FRET in the absence of the test compound indicates that the test compound modulates SERCA, such that the energy transfer between the two chromophores is altered.