Patent ID: 6458584
Filing Date: 2002-10-01
Classification: B01J,C12Q,G16B

Abstract:
A method for using a reuseable microchip to identify at least one nucleic acid sequence in nucleic acids of a sample, said method comprising:(a) providing a customized matrix of oligonucleotides on the microchip in an arrangement that is an ordered scheme designed to present a detectable pattern as a whole that identifies the sequences of nucleic acids in the sample; (b) hybridizing nucleic acids from the sample on said microchip; and (c) identifying at least one nucleic acid sequence in said sample by the pattern detected as a whole on the microchip, said pattern resulting from the locations of oligonucleotides which hybridized to the sample nucleic acids wherein the customized matrix of oligonucleotides on the reusable microchip is formed by a plurality of gel elements, wherein the number of elements is determined by the number of oligonucleotides in the matrix and wherein each gel element contains one oligonucleotide of a desired nucleic acid sequence length and concentration, each gel element being separated from another by interstitial spaces, said oligonucleotides being positioned in specific locations and wherein said matrix comprises oligonucleotides comprising the base sequences:Oligonu-cleotide Nameand PositionSequence (5â€² to 3â€²)Nb1000TGC GAC CGG TCA TGG(SEQ ID: 6)NIT3CCT GTG CTC CAT GCT CCG(SEQ ID: 7)NEU23CCC CTC TGC TGC ACT CTA(SEQ ID: 8)NSO190CGA TCC CCT GCT TTT CTC(SEQ ID: 9)NSO1225CGC GAT TGT ATT ACG TGT GA(SEQ ID NO: 10)NSMO156TAT TAG CAC ATC TTT CGA T(SEQ ID NO: 11)NSV443CCG TGA CCG TTT CGT TCC(SEQ ID NO: 12)BAC338GCT GCC TCC CGT AGG GAT(SEQ ID NO: 13)NonBAC338ACT CCT ACG GGA GGC AGC(SEQ ID NO: 14)UNI1390GAC GGG CGG TGT GTA CAA(SEQ ID NO: 15)