Patent ID: 6093554
Filing Date: 2000-07-25
Classification: C07K,C12N

Abstract:
A method of inducible production in plants or plant cells of a non-viral sequence of interest selected from the group consisting of proteins, polypeptides and RNAs, comprisinga) obtaining a nucleotide sequence derived from an RNA-based plant virus which for its replication is dependent upon a viral RNA/RNA polymerase, said nucleotide sequence representing a stripped viral replicon comprising cis elements for replication but being devoid of genes coding for viral RNA/RNA polymerase and viral coat protein, said stripped viral replicon not being able to replicated independently but in the presence of the viral RNA/RNA polymerase replicating more efficiently than the RNA of the wild-type plant virus,b) preparing a recombinant DNA comprising an expression cassette functional in plants or plant cells, said expression cassette containing, between two inverted repeat nucleotide sequences of 12-250 bp, DNA corresponding to said RNA virus-derived nucleotide sequence and a nucleotide sequence encoding the non-viral sequence of interest to be produced,c) performing a genetic manipulation of the plants or plant cells to incorporate said recombinant DNA into the genome of said plants or plant cells, andd) growing said plants or plant cells and causing the presence of a viral RNA/RNA polymerase to induce or amplify the expression of the non-viral sequence of interest.