Patent ID: 6046005
Filing Date: 2000-04-04
Classification: C12Q

Abstract:
A method of determining the sequence of a nucleic acid, said method comprising:(a) preparing a reaction mixture corresponding to each base of said nucleic acid, wherein each of said reaction mixtures comprises a family of enzymatically produced capturable primer extension products produced by combining said nucleic acid, polymerase, deoxynucleotides, oligonucleotide primer and capturable dideoxy chain terminator in a reaction mixture under conditions sufficient to produce said capturable primer extension products, wherein said capturable dideoxy chain terminator comprises a capture moiety bonded to a dideoxynucleotide through a cleavable linking group.(b) contacting each of said reaction mixtures with a solid phase capable of capturing said primer extension products comprising said capturable chain terminator through interaction of said capture moiety with a moiety present on said solid phase and to produce solid phase captured primer extension products;(c) separating said solid phase captured primer extension products from said reaction mixture;(d) releasing said primer extension products from said solid phase;(e) size separating and detecting said released primer extension products to produce sequencing data; and(f) determining the sequence of said nucleic acid from said sequencing data.