Patent ID: 6376194
Filing Date: 2002-04-23
Classification: B01J,C12N,Y10S,Y10T

Abstract:
A method of isolating a target nucleic acid from a mixture comprising the target nucleic acid and at least one contaminant, comprising:a) providing a mixed-bed solid phase comprising a first solid phase and a second solid phase, wherein: the first solid phase has the capacity to bind to the target nucleic acid in a first solution and to release the target nucleic acid bound thereto in the presence of a second solution, the second solid phase has the capacity to bind to the target nucleic acid in the second solution and to release the target nucleic acid bound thereto in the presence of the first solution, and the first solid phase and the second solid phase each have the capacity to release the target nucleic acid bound thereto in the presence of an elution buffer; b) combining the mixture with the mixed-bed solid phase in the presence of the first solution and permitting the target nucleic acid to bind to the first solid phase; c) separating the mixed-bed solid phase from the first solution; d) combining the mixed-bed solid phase with the second solution, and permitting the target nucleic acid to release from the first solid phase and bind to the second solid phase; e) separating the mixed-bed solid phase from the second solution; and f) combining the mixed-bed solid phase with the elution buffer, and permitting the target nucleic acid to release from the mixed-bed solid phase into the elution buffer.