Patent ID: 8383409
Filing Date: 2013-02-26
Classification: C08G,C08L,C12N

Abstract:
1. A method for the propagation or cultivation of cells comprising: preparing a cell culture substrate, wherein said cell culture substrate consists of a substrate and a surface modification layer, said layer consisting of a polymer comprising an amino group, wherein said polymer comprising said amino group is produced by reacting a polymer represented by the following formula (II): with a polymer having at least one amino group, —NH 2 , capable of forming a Schiff base in a monomer of formula II, thereby forming a polymer layer constituting the layer formed by surface modification, wherein n is 0 or a positive integer, and m is a positive integer, the n and m representing degree of polymerization, wherein the surface modification layer improves affinity and adhesiveness of a cell, and wherein said formula (II) is formed by chemical vapor deposition of formyl[2.2]paracyclophane represented by the following formula (I): wherein k is 0 or 1; providing cells in a medium; inoculating the cells in said medium onto the cell culture substrate; and culturing the inoculated cells, wherein the cells adhere to the cell culture substrate.