Patent ID: 6777202
Filing Date: 2004-08-17
Classification: A61K,A61P,C07K,C12N

Abstract:
A process for production of a crystalline S-layer protein comprising:(a) transforming a gram-negative prokaryotic host cell with a full length nucleic acid encoding an S-layer protein selected from the group consisting of (i) a nucleic acid comprising a nucleotide sequence from position 1 to 3684 of SEQ ID NO:1, (ii) a nucleic acid comprising a nucleotide sequence which encodes an amino acid sequence according to SEQ ID NO:2, and (iii) a nucleic acid comprising a nucleotide sequence which hybridizes with at least one of the nucleic acid of (i) or (ii) under stringent conditions; (b) culturing the host cell under conditions which induce expression of the nucleic acid and production of the corresponding protein, and (c) isolating the protein from the host cell.