Patent ID: 6251615
Filing Date: 2001-06-26
Classification: G01N,Y10S,Y10T

Abstract:
A method of analyzing cells in a carrier solution, comprising:(a) introducing said carrier solution into a conduit having a surface portion wherein the surface portion has a discreet capture zone formed therein, said carrier solution having a plurality of cells suspended therein;(b) contacting said plurality of said cells to said surface portion, said surface portion containing at least one imaging field;(c) sequentially interrogating said plurality of cells in said imaging field with at least two different types of emitted light;(d) processing resultant light from said imaging field for each of said at least two different types of emitted light;(e) generating digital information for each of said plurality of cells from said resultant light for each of said at least two different types of emitted light; and then(f) generating a response file for each of said plurality of cells from said digital information for each of said at least two different types of emitted light;and wherein said sequentially interrogating step is followed by the steps of: altering the temperature of said cells in said capture zone; and then repeating said sequentially interrogating step.