Patent ID: 8883445
Filing Date: 2014-11-11
Classification: C12N,C12Y

Abstract:
1. A method of reducing glycosylation on proteins produced from methylotrophic yeast, comprising providing a methylotrophic yeast strain, which strain is transformed with a nucleic acid coding for a protein which comprises a GlcNAc-transferase I (GnT1) or an enzymatically active fragment thereof wherein said GnT1 is a human, rat or rabbit GnT1, and which strain is also transformed with a nucleic acid coding for a protein which comprises an α-1,2-mannosidase or an enzymatically active fragment thereof, wherein said α-1,2-mannosidase is T. reesei α-1,2-mannosidase, and wherein said α-1,2-mannosidase and said enzymatically active fragment are engineered to contain an ER localization signal and convert Man 8 GlcNAc 2 to Man 5 GlcNAc 2 ; and producing glycoproteins from said methylotrophic yeast strain.