Patent ID: 6451617
Filing Date: 2002-09-17
Classification: G01N

Abstract:
A method for screening substances that inhibit binding between a transforming growth factor-&bgr;-activated kinase 1 (TAB1) polypeptide and TAK-1-binding (TAB1) polypeptide, wherein the TAK1 polypeptide has the amino acid sequence comprising Val at amino acid position 76 to Gln at amino acid position 303 as set forth in SEQ ID NO: 4 and the TAB 1 polypeptide has the amino acid sequence comprising Gln at amino acid position 437 to Pro at amino acid position 504 as set forth in SEQ ID NO: 2, which method comprises:(1)(a) contacting the TAK1 polypeptide and the TAB1 polypeptide with a test sample, and detecting or determining the amount of the TAK1 polypeptide that is bound to the TAB1 polypeptide, (b) contacting the TAK1 polypeptide with the TAB1 polypeptide in the absence of a test sample, and detecting or determining the amount of the TAK1 polypeptide that is bound to the TAB1 polypeptide; and then (2) comparing the amount of bound TAK1 polypeptide obtained in the step (1)(a) to the amount of bound TAK1 polypeptide obtained in the step (1)(b).