Patent ID: 6500933
Filing Date: 2002-12-31
Classification: A61K,C07K,C12N,Y10S

Abstract:
A method for crosslinking a glycoprotein crystal, said method comprising the steps of:(a) crosslinking a glycoprotein crystal with a first crosslinking agent through one or more carbohydrate moieties of said glycoprotein; and (b) crosslinking said crosslinked glycoprotein crystal with at least a second crosslinking agent through the one or more carbohydrate moieties of said glycoprotein, said second crosslinking agent being different from said first crosslinking agent; wherein the crosslinked glycoprotein crystal displays a three dimensional crystal lattice, is a shape selected from the group consisting of spheres, needles, rods, plates, rhomboids, cubes, bipyramids and prisms, has enhanced stability as compared to a non-crosslinked counterpart glycoprotein crystal and said glycoprotein is selected from the group consisting of lipases and oxidases.