Patent ID: 8465912
Filing Date: 2013-06-18
Classification: A61P,C12N,G01N

Abstract:
1. A method of identifying an agent that inhibits tumor cells from undergoing an epithelial to mesenchymal transition, comprising: contacting a sample of cells of the epithelial tumor cell line H1650 with a test agent to be screened, contacting the sample with a single or dual protein ligand preparation that induces an epithelial-to-mesenchymal transition in H1650 cells, determining whether the test agent inhibits the tumor cells in the sample from undergoing an epithelial to mesenchymal transition, by comparing the level of a biomarker whose level is indicative of the EMT status of the sample tumor cells to the level of the same biomarker in an identical sample of identically treated H1650 cells not contacted with the test agent, and thus determining whether the test agent is an agent that inhibits tumor cells from undergoing an epithelial to mesenchymal transition.