Patent ID: 6296846
Filing Date: 2001-10-02
Classification: A61K,A61P,C07K

Abstract:
A method of inducing xenogeneic tolerance in a recipient primate to a swine graft comprising:introducing into said recipient primate an antibody preparation which eliminates mature T cells and NK cells of said recipient primate;irradiating said recipient primate with low dose irradiation, said irradiation being non-myeloablative and being sufficient to partially deplete said recipient primate's endogenous bone marrow cells in order to stimulate hematopoiesis of newly introduced swine hematopoietic stem cells;removing natural anti-xenogeneic antibodies from the blood of said recipient;introducing into said recipient primate a preparation which comprises donor swine hematopoietic stem cells which develop into mature myeloid or lymphoid cells, or both, and which form a chimeric lymphohematopoietic population in said recipient primate, thereby inducing immunological tolerance in said recipient primate to a swine graft; andintroducing into the recipient primate a swine graft,wherein the same swine, a genetically matched swine, or a swine from a highly inbred herd, is the donor of both the graft and the hematopoietic cells.