Patent ID: 8377671
Filing Date: 2013-02-19
Classification: C12N

Abstract:
1. A process for obtaining a mutant polynucleotide encoding a modified large subunit of a wild-type O 2 sensitive [NiFe]-hydrogenase, for improving dioxygen tolerance of said [NiFe]-hydrogenase, wherein said process comprises: providing an initial polynucleotide comprising a sequence encoding a large subunit of a wild-type O L1: RGXE, wherein X=L, I, F, V or M L2: [R/K]X L3: X L4: D[P/I/S]CX and optionally comprising a motif L0: R[I/V/A]EG[H/D/A]; modifying said initial polynucleotide in order to substitute at least the residues X