Patent ID: 8017344
Filing Date: 2011-09-13
Classification: A61P,C07K,G01N

Abstract:
1. A method of screening for an inhibitor of the binding between a CAR and a CARL, which comprises the steps of: (a) contacting a CAR and a CARL in the presence of a test substance; (b) detecting the binding between the CAR and the CARL in step (a); (c) comparing the degree of binding between the CAR and the CARL, detected in step (b), with that in the absence of the test substance; and (d) selecting as an inhibitor to the binding between CAR and CARL from the test substance that (a) suppresses the binding between the CAR and the CARL and (b) suppresses the cell adhesion between Th1 cells expressing CARL and epithelial cells and endothelial cells expressing CAR, compared to in the absence of the test substance; wherein the CARL is a protein that binds to a natural CAR, and is the protein of any one of: (1) a protein comprising the amino acid sequence of SEQ ID NO: 1; (2) a protein comprising an extracellular domain of the amino acid sequence of SEQ ID NO: 1; (3) a protein comprising the amino acid sequence of SEQ ID NO: 2; (4) a protein comprising an extracellular domain of the amino acid sequence of SEQ ID NO: 2; (5) a protein comprising an Ig-like domain 1 of the amino acid sequence of an above (1) to (4); or (6) a fusion protein between a marker protein and a protein of the above (1) to (5); and wherein the CAR is a protein that binds to a polypeptide comprising the amino acid sequence of SEQ ID NO: 1 or 2, and is a protein comprising an amino acid sequence of any one of: (7) the amino acid sequence of (SEQ ID NO:17) or (SEQ ID NO:18); (8) an amino acid sequence comprising an extracellular domain of an amino acid sequence of the above (7); or (9) a fusion amino acid sequence between a marker protein and the protein of the above (7) to (8).