Patent ID: 6440736
Filing Date: 2002-08-27
Classification: A61K,A61P,C07K,C12N

Abstract:
A process for altering the properties of a target cell and/or a target cellular membrane through the direct introduction of a first heterologous protein to the target cell and/or target cellular membrane surface comprising contacting said target cell and/or target cellular membrane with an effective amount of a lipid-modified protein,wherein said lipid-modified protein is enzymatically synthesized to contain bound lipid either in a cell or in a cell-free system from a fusion protein that has at least one first portion derived from said first heterologous protein and at least one second portion comprising a lipidation signal derived from a second protein, wherein said second protein is a lipoprotein, wherein the effective amount is sufficient to impart to the target cell and/or target cell membrane surface an additional characteristic resulting from the first heterologous portion, and the lipid bound to the lipid-modified protein attaches the lipid-modified protein directly to the target cell and/or target cellular membrane.