Patent ID: 6667175
Filing Date: 2003-12-23
Classification: A61K,C12N,G01N

Abstract:
A method of generating allopeptide antigen specific human suppressor CD8+CD28âˆ’ T cells which comprises:a) obtaining peripheral blood T cells from a subject; b) stimulating by multiple priming a T cell line from the T cells obtained in step (a) with autologous antigen presenting cells (APCs) pulsed with an allopeptide, said allopeptide comprising an amino acid sequence comprising both MHC class I and MHC class II amino acid sequences wherein the amino acid sequences are binding sequences and are recognized by the primed T cell line; c) isolating primed populations of CD8+ T cells and of CD4+ T helper cells from the T cell line stimulated in step (b); d) isolating primed CD8+CD28âˆ’ T cells from the isolated primed CD8+ T cells of step (c); e) detecting suppression by the primed CD8+CD28âˆ’ T cells isolated in step (d) of interaction between the CD4+ T helper cells isolated in step (c) and autologous antigen presenting cells (APCs) expressing the same MHC class I and MHC class II binding motifs as expressed by the APCs used to stimulate the T cell line of step (b), thereby identifying allopeptide antigen specific human suppressor CD8+CD28âˆ’ T cells; and f) expanding the allopeptide antigen specific human suppressor CD8+CD28âˆ’ T cells identified in step (e), thereby generating allopeptide antigen specific human suppressor CD8+CD28âˆ’ T cells.