Patent ID: 9175339
Filing Date: 2015-11-03
Classification: C12Q

Abstract:
1. A method for detection of a target nucleic acid in a sample, comprising: preparing a solid phase comprising detection probes respectively having different base sequences; carrying out PCR on the sample to obtain chimeric DNAs each having a label, and a tag sequence complementary to each of the detection probes having been correlated to the target nucleic acid; bringing the chimeric DNAs into contact with the detection probes such that the chimeric DNAs and the detection probes can hybridize through the tag sequences; obtaining signal intensity information based on the label on the solid phase; and detecting the target nucleic acid based on the signal intensity information; wherein the step of PCR comprises: preparing a set of primers which does not include a universal primer, wherein the set of primers consists of a first primer having an identification sequence complementary to a target sequence in the target nucleic acid and a tag addition sequence complementary to the tag sequence, and a second primer having a partial sequence that has the same sequence as a partial sequence adjacent to the target sequence and the label; and carrying out PCR on the sample using a set consisting of the first primer and the second primer to synthesize the chimeric DNA having the target sequence, the tag sequence and the label.