Patent ID: 6114125
Filing Date: 2000-09-05
Classification: A61K,C07K,C12N

Abstract:
A method of determining the presence of Haemophilus influenzae Hin47 protein in a sample, comprising the steps of:(a) immunizing a subject with an immunogenic composition comprising an immunoeffective amount of (1) an isolated and purified analog of Haemophilus influenzae Hin47 having a decreased protease activity which is less than about 10% of that of natural Hin47 protein and having substantially the same immunological properties as natural Hin47 protein or (2) an isolated and purified nucleic acid molecule comprising a mutant Haemophilus influenzae Hin47 gene encoding an analog of Haemophilus influenzae Hin47 protein having a decreased protease activity which is less than about 10% of that of natural Hin47 protein and having substantially the immunogenic properties of natural Hin47 protein to produce antibodies specifically reactive therewith,(b) contacting the sample with the antibodies to produce complexes comprising any Hin47 protein present in the sample and said Hin47 protein specific antibodies; and(c) determining production of the complexes.