Patent ID: 6379898
Filing Date: 2002-04-30
Classification: C12Q,Y10S

Abstract:
A method for determining the presence and/or amount of cells and cellular material, the method comprising:releasing the contents of cells to form a cell lysate; adding phosphate donor molecules and adenosine 5â€²-monophosphate molecules to the cell lysate so that adenosine 5â€²-diphosphate molecules are produced by the enzymatic transfer of a phosphate group from the donor to the adenosine 5â€²-monophosphate according to the following general reaction catalyzed by endogenous enzymes present in the cell lysate: Dâˆ’P+AMPâ†’D+ADP; â€ƒand producing adenosine 5â€²-triphosphate by the enzymatic transfer of a phosphate group from the donor molecules to the adenosine 5â€²-diphosphate molecules according to the following general reaction catalyzed by endogenous enzymes present in the cell lysate sample: Dâˆ’P+ADPâ†’D+ATP; â€ƒand detecting the adenosine 5â€²-triphosphate produced thereby.