Patent ID: 7932059
Filing Date: 2011-04-26
Classification: C12Q

Abstract:
1. A reaction mixture for primer-based amplification and detection of a target nucleic acid, the reaction mixture comprising: each conventional nucleotide dATP, dCTP, and dGTP, and a combination of unconjugated dUTP and dTTP in an amount equivalent to the concentrations of dATP, dCTP and dGTP, wherein said unconjugated dUTP replaces from about 10% to about 75% of said dTTP in said combination; and at least one of a fluorescent probe, beacon or intercalating dye; wherein the inclusion of unconjugated dUTP reduces the formation of primer aggregates during the amplification reaction in comparison with an amplification reaction employing only conventional nucleotides; and wherein said reaction mixture lacks a uracil degradation enzyme.