Patent ID: 8728757
Filing Date: 2014-05-20
Classification: A61K,C07K,C12N,G01N

Abstract:
1. A method for identifying a compound that modulates a cool or cold sensation in a mammalian subject, the method comprising: (i) contacting a cell comprising a cold- and menthol-sensitive receptor (CMR1) polypeptide with the compound, wherein the CMR1polypeptide comprises a sequence having at least 90% identity to SEQ ID NO:3 and forms a cation channel; and (ii) determining whether said compound that modulates the activity of said CMR1polypeptide based on an effect in said cell that correlates to CMR1activity, and (iii) if said compound elicits the effect in said cell, further determining whether said compound modulates a cool/cold sensation in a mammalian subject, thereby identifying a compound that modulates cool or cold-sensation.