Patent ID: 8871461
Filing Date: 2014-10-28
Classification: C12N

Abstract:
1. A method of mimicking hemodynamic flow during cell culture, said method comprising the steps of: adding a culture media to a Petri dish; plating a first cell type on a first side of a porous membrane; plating a second cell type on a second side of the porous membrane, wherein said porous membrane is suspended in the Petri dish such that the first side is proximal and in spaced relation to a surface of the Petri dish, thereby defining within the Petri dish a lower volume comprising the first cell type and an upper volume comprising the second cell type, the porous membrane being adapted to permit fluid communication of the culture media and physical interaction and communication between cells of the first cell type and cells of the second cell type, and all of the cell types are within the culture media; perfusing culture media into and out of the upper volume through inlets and outlets within the portion of the Petri dish defining the upper volume; perfusing culture media into and out of the lower volume through inlets and outlets within the portion of the Petri dish defining the lower volume; and applying a shear force upon the plated second cell type, said shear force resulting from flow of the culture media induced by a hemodynamic flow device, said flow mimicking hemodynamic flow; wherein at least one of the first cell type and the second cell type is a vascular cell type.