Patent ID: 8444934
Filing Date: 2013-05-21
Classification: B01J,B01L,G01N,Y10T

Abstract:
1. A microfluidic device comprising: a microfluidic flow cell; and a hydrophilic substrate for being removably interfaced with said microfluidic flow cell for performing a reaction therebetween without any adhesive therebetween, said substrate comprising a flat contiguous surface for removably superimposing said microfluidic flow cell thereon and providing for analyzing a reaction product thereon, said microfluidic flow cell providing for being removably superimposed on said substrate surface and comprising: an elongated body defining opposite front and rear ends, opposite lateral sides, a first surface for being interfaced with said substrate surface and an opposite second surface: at least one reaction portion formed on said first surface at least near said front end and defining with said substrate surface a reaction chamber when said first surface is interfaced with said substrate surface; a common channel-cavity formed on said first surface and positioned generally centrally of said elongated body and defining with said substrate surface a common channel in fluid communication with said reaction chamber when said first surface is interfaced with said substrate surface; at least two separate conduit cavities formed on said first surface and defining with said substrate surface respective separate conduits in fluid communication with said common channel and extending therefrom towards a respective one of said opposite lateral sides and towards said rear end when said first surface is interfaced with said substrate surface for providing fluids therein to move both forwardly relative to said rear end and inwardly relative to said lateral side; at least two separate fluid receiving portions having respective openings formed on said second surface and positioned at least near said rear end for receiving a fluid therein, each said fluid receiving portion being in fluid communication with a respective said conduit; a valve cavity formed on said first surface and defining a valve with said substrate surface in fluid communication with said common channel and with said conduits when said first surface is interfaced with said substrate surface, said valve being positioned relative to the length of said common channel downstream of said fluid receiving portions; and a dispensing portion in fluid communication with said reaction chamber, and with the external environment of said microfluidic flow cell, said dispensing portion comprising a dispensing channel formed within said microfluidic flow cell; wherein when said microfluidic flow cell and said substrate are interfaced, said microlfuidic device is adapted, when submitted to centrifugal forces to allow for the fluid in each said fluid-receiving portion to flow to said reaction chamber and for excess fluid in said reaction chamber to flow into the external environment via said dispensing portion, wherein said valve is configured to burst at a predetermined rotation speed and wherein when the reaction is completed, said microfluidic flow cell being so removable from said substrate so as to allow the reaction product to remain on said substrate.