Patent ID: 9035128
Filing Date: 2015-05-19
Classification: A01K,C07K,C12N

Abstract:
1. A mouse comprising at an endogenous κ light chain locus in its germline: (a) an unrearranged light chain variable region comprising a plurality of contiguous unrearranged functional human λ light chain V (hVλ) gene segments and a plurality of contiguous unrearranged functional human λ light chain J (hJ λ) gene segments, wherein the plurality of hV λ gene segments and the plurality of hJ λ gene segments are the only functional variable region gene segments in the unrearranged light chain variable region; and (b) a mouse κ light chain constant region gene, wherein the plurality of contiguous unrearranged functional human λ light chain V (hVλ) gene segments and the plurality of contiguous unrearranged functional human λ light chain J (hJ λ) gene segments are operably linked to the mouse κ light chain constant region gene such that the unrearranged light chain variable region is capable of rearranging to form a rearranged human λ light chain variable region and the mouse expresses antibodies comprising a light chain comprising a variable region encoded by the rearranged human λ light chain variable region and a constant region encoded by the mouse κ light chain constant region gene.