Patent ID: 6787308
Filing Date: 2004-09-07
Classification: B01J,C12Q,C40B

Abstract:
An array of nucleic acids comprising;a) a support having a solid surface; b) a plurality of distinct optically resolvable polynucleotide molecules immobilized at discrete sites on said solid surface; c) an attachment between said solid surface and each of said polynucleotide molecules for the immobilization of said polynucleotide molecules on said solid surface; d) wherein each of said polynucleotide molecules comprises a polynucleotide duplex covalently linked to form a hairpin loop structure; e) wherein one end of said polynucleotide duplex comprises a target polynucleotide and each polynucleotide duplex is an individually resolvable molecule detectable as a single molecule fluorescent point; and f) wherein fluorescence from said single molecule fluorescent point exhibits single step photobleaching and said attachment does not exhibit fluorescence.