Patent ID: 6878526
Filing Date: 2005-04-12
Classification: A61P,G01N

Abstract:
1. A method for the diagnosis of a cancerous state in a subject which method comprises: (a) obtaining a sample of p53 mutant cells or an extract therefrom from said subject; (b) measuring the optical density per 150 μg total cellular protein of CDK1 and CDK4 in said sample; (c) normalizing the optical density of CDK1 per 150 μg total cellular protein measured in step (b) by dividing the measured optical density by the mean optical density of CDK1 per 150 μg total cellular protein and multiplying by 5; (d) normalizing the optical density of CDK4 per 150 μg total cellular protein measured in step (b) by dividing the measured optical density by the mean optical density of CDK4 per 150 μg total cellular protein and multiplying by 5; (e) determining the ratio of the normalized optical density of CDK1 to the normalized optical density of CDK4; and (f) diagnosing cancer when the ratio of CDK1 to CDK4 determined in step (e) is between 1.0 and 1.6, wherein said mean optical density of CDK1 per 150 μg total cellular protein is the mean optical density of CDK1 from a number of samples of p53 mutant cancer cells or an extract thereof, and wherein said mean optical density of CDK4 per 150 μg total cellular protein is the mean optical density of CDK4 from a number of samples of p53 mutant cancer cells or an extract therefrom.