Patent ID: 9187549
Filing Date: 2015-11-17
Classification: C07K

Abstract:
1. A method for reducing the concentration of Protein S in a composition comprising a recombinant Vitamin K-dependent protein of interest produced under cell culturing conditions, the method comprising: (A) contacting a cell culture supernatant composition comprising a recombinant Vitamin K-dependent protein of interest and Protein S, wherein the recombinant Vitamin K-dependent protein of interest is produced under cell culturing conditions with a chromatographic column comprising a solid phase material canying monoclonal antibodies against the Vitamin K-dependent protein of interest, wherein the Protein S is present in the composition at an initial concentration of 500 to 1000 parts per million (ppm) relative to the Vitamin K-dependent protein of interest; (B) collecting a resulting composition comprising the Vitamin-K dependent protein of interest and Protein S; (C) contacting the resulting composition with a chromatographic column comprising a solid phase material carrying monoclonal antibodies against Protein S, wherein the monoclonal antibodies against Protein S selectively bind Protein S but do not bind the recombinant Vitamin K-dependent protein of interest to the solid phase material thereby allowing the Vitamin K-dependent protein of interest to flow through the chromatographic column; and (D) collecting the resulting composition comprising the Vitamin-K dependent protein of interest and Protein S, wherein the concentration of Protein S in the resulting composition expressed as parts per million relative to the recombinant Vitamin K-dependent protein of interest is reduced to less than or equal to 100 ppm.