Patent ID: 8053185
Filing Date: 2011-11-08
Classification: B01F,B01J,B01L,C12Q,C40B,H01J,Y02P

Abstract:
1. A method for simultaneously conducting a plurality of sequence-specific micro-volume polynucleotide amplification reactions to provide molecular haplotype information for a target nucleic acid sequence, the method comprising: (a) introducing a plurality of liquid samples comprising a target nucleic acid into the sample chambers of a microhole apparatus, wherein the liquid samples are terminally diluted, such that on average, some of the sample chambers contain exactly one copy of the target nucleic acid, and wherein the chambers contain necessary polynucleotide amplification reaction components; (b) submerging the microhole apparatus in a hydrophobic medium during the plurality of micro-volume polynucleotide amplification reactions; and (c) placing the submerged microhole apparatus into an environment favorable to simultaneously conducting a plurality of sequence-specific micro-volume polynucleotide amplification reactions on the plurality of liquid samples, wherein the microhole apparatus comprises a substrate, wherein the substrate is solid and defines a plurality of sample chambers, wherein each sample chamber: