Patent ID: 6727070
Filing Date: 2004-04-27
Classification: C07K,G01N

Abstract:
A method for determining proper folding and/or solubility of a protein of interest comprising:a) providing an expression construct comprising (i) a gene encoding a first polypeptide comprising said protein of interest fused to a first segment of a marker protein, and (ii) a promoter active in a selected host cell and operably linked to said gene; b) expressing said first polypeptide in said host cell that also expresses a second segment of said marker protein, wherein said second marker segment combines with said first marker segment to form a functional marker protein when said first polypeptide is properly folded; and c) measuring marker protein function, wherein a greater degree of marker protein function, as compared to marker protein function observed with appropiate negative controls, indicates proper folding and/or solubility of said protein of interest.