Patent ID: 8535884
Filing Date: 2013-09-17
Classification: C07K,C12Q,G01N,Y10T

Abstract:
1. A method of detecting arteriosclerosis, atherosclerosis, predisposition to arteriosclerosis, or predisposition to atherosclerosis in a subject, the method comprising: detecting in a sample from the subject, expression of a truncated Lamin A protein or mutant LMNA nucleic acid encoding the truncated Lamin A protein by reacting at least one mutant LMNA nucleic acid molecule in the sample from the subject with a reagent comprising a LMNA nucleic acid-specific binding agent to form a mutant LMNA nucleic acid:agent complex, wherein the truncated Lamin A protein comprises: (a) the amino acid sequence set forth as SEQ ID NO: 7; (b) an amino acid sequence having at least 95% sequence identity to SEQ ID NO: 7 and containing Mutation 1 (G608G(GGC>GGT)) or Mutation 2 (G608S(GGC>AGC)); or (c) a conservative variant of (a) containing Mutation 1 (G608G(GGC>GGT)) or Mutation 2 (G608S(GGC>AGC)); whereby the expression of the truncated Lamin A protein or mutant LMNA nucleic acid indicates that the subject has or is predisposed to arteriosclerosis or atherosclerosis.