Patent ID: 9221900
Filing Date: 2015-12-29
Classification: A61P,C07K,G01N

Abstract:
1. A method for identifying an anti-beta amyloid antibody having neuroprotective activity, wherein the method comprises: a. incubating microglia cells with beta amyloid oligomer and the anti-beta amyloid antibody; and b. measuring the uptake of beta amyloid oligomer by microglia cells; and c. measuring p38 MAP kinase activation in the microglia cells, and d. identifying the uptake of beta amyloid oligomer into the microglia cells and an intermediate level of p38 MAP kinase activation in the microglia cells incubated with the anti-beta amyloid antibody as an indication of an anti-beta amyloid antibody having neuroprotective activity, wherein the intermediate level of p38 MAP kinase activation is a level higher than a level of activation of p38 MAP kinase in the presence of beta amyloid oligomer without the anti-beta amyloid antibody, but lower than a level of activation of p38 MAP kinase in the presence of beta amyloid oligomer and an IgG1 antibody that specifically binds beta amyloid oligomer, and wherein the IgG1 antibody comprises a human IgG1 constant region.