Patent ID: 6756226
Filing Date: 2004-06-29
Classification: A01K,C12N

Abstract:
A method for making an infectious adenovirus which comprises contacting a cell with or introducing into a cell:a. a first nucleic acid sequence being a plasmid comprising a circularized adenovirus DNA molecule having a deletion of an adenoviral packaging signal, and which, by itself, in the absence of intermolecular recombination, is incapable of generating an infectious, packageable adenovirus; and b. a second nucleic acid sequence which, by itself, in the absence of intermolecular recombination, is incapable of generating an infectious, packageable adenovirus, and encoding adenovirus sequences which, in the absence of adenoviral replication factors provided in trans or intermolecular recombination with said first nucleic acid sequence, are incapable of encoding an infectious, packageable adenovirus; provided that said first and said second nucleic acid sequences each comprise a head-to-head ITR junction and sufficient overlapping adenoviral nucleic acid sequences such that homologous recombination may occur between said first and said second nucleic acid sequences, whereby said first and said second nucleic acids recombine to form said infectious adenovirus.