Patent ID: 6087149
Filing Date: 2000-07-11
Classification: C12N,C12P,C12Y

Abstract:
An isolated nucleic acid which comprises a sequence selected from the group consisting of:(a) a sequence which is present in plasmid pUC19 in Escherichia coli DSM 11971 and which encodes an isoamylase or a fragment thereof having isoamylase activity;(b) a sequence of nucleotides 1-2178 of SEQ ID NO:3 or its complementary strand;(c) a sequence encoding a polypeptide having an amino acid sequence of SEQ ID NO:4 or a fragment thereof having isoamylase activity;(d) a sequence which encodes an isoamylase and which is at least 80% homologous with sequences (a) or (b), wherein the homology is determined using GAP with a GAP creation penalty of 5.0 and a GAP extension penalty of 0.3; and(e) a sequence which encodes a polypeptide having isoamylase activity and hybridizes under medium stringency conditions with nucleotides 1-2178 of SEQ ID NO:3.