Patent ID: 6670188
Filing Date: 2003-12-30
Classification: A61K,C07K,C12N

Abstract:
A method for generating recombinant adenovirus having a gene of interest, said method comprising:providing first and second recombinant nucleic acid sequences lacking overlapping sequences with respect to a packaging cell, said first recombinant nucleic acid sequence comprising an ITR, a packaging signal and the gene of interest, and sequences having sufficient homology with said second recombinant nucleic acid sequence to allow for homologous recombination between said first and second recombinant nucleic acid sequences in a suitable environment, said second recombinant nucleic acid further comprising adenoviral sequences necessary for generating a recombinant adenovirus; bringing said first and second recombinant nucleic acid sequences into the suitable environment, wherein said suitable environment comprises the packaging cell, the packaging cell comprising the adenoviral sequence replaced by the gene of interest in the first recombinant nucleic acid sequence; allowing for homologous recombination; and producing recombinant adenovirus without the concomitant production of adenovirus containing transforming adenoviral sequences and/or replication competent adenovirus.