Patent ID: 8709772
Filing Date: 2014-04-29
Classification: A21D,A61K,A61P,C12N,C12P,C12Q,C12Y,D06L,D06M,G01N,Y02E,Y02P,Y02T

Abstract:
1. An isolated, synthetic, or recombinant polypeptide having a d-amino-acid transferase activity, wherein the polypeptide is the amino acid sequence of SEQ ID NO:220 or the polypeptide is selected from a group consisting of: (a) a polypeptide variant having d-amino-acid transferase activity wherein the polypeptide variant has at least about 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or 100% sequence identity to the full length of the amino acid sequence of SEQ ID NO:220; (b) a polypeptide having d-amino-acid transferase activity and the amino acid sequence encoded by SEQ ID NO:219 (c) a polypeptide having d-amino-acid transferase activity wherein the polypeptide is a variant of the amino acid sequence of SEQ ID NO:220 and at least one mutation of SEQ ID NO:220 selected from the group consisting of: D2A, D2D, D2F, D2G, D2H, D2I, D2N, D2Q, D2R, D2T, D2V, D2Y, Y6C, Y6F, Y6H, Y6L, Y6M, G9A, N10R, N10S, N10T, N10W, G12A, L14L, L14V, T18W, V19T, P20S, N40N, G41G, G41Y, V42M, V42V, A45L, L46A, L46H, D47W, N55L, S56S, A57M, E61D, E61R, E61Y, 162C, 164H, L66G, L66L, E69Y, K72M, K73L, T74A, T74L, T74M, T74R, T74V, N76C, E77R, Y79R, Y79V, S80L, S80R, V82A, G85D, G85G, V93A, V93G, V93L, V93Y, G98A, S101Q, S101S, V103T, A106G, A106W, P108C, P108F, P108T, N110A, P117W, N118A, N118G, N118K, N118R, I120L, K126K, K126Q, K126R, I127M, K128L, K128R, K128S, L129I, L129V, I130M, T131T, M132A, M132R, M132Y, D133E, D133L, D133N, F137W, L138L, P149P, N150S, I152V, R156A, R156W, G161G, H163K, H163R, A165T, H168A, E171R, E171S, T172I, C176G, A177S, H182G, K185R, K185T, N186S, S205A, Q209S, V212E, R213W, I216T, P217H, P217V, D219F, E220V, R221E, F223C, S226P, L228F, V234A, V236T, S238S, G240G, T241C, T241R, L242F, E248F, D250E, G256K, K257V, E260G, L262R, K263M, I265L, D267G, D267R, E268S, L270G, L270S, L270W, R271S, I274W, G278S, Y279C, T280N, P281W, E282G, W283A, W283E, W283G, W283R, W283S, W283T, W283V, S284R, R285F, V286G, and V286R; or, (d) a polypeptide having d-amino-acid transferase activity wherein the polypeptide is a variant of the amino acid sequence of SEQ ID NO:220 and a combination of mutations of SEQ ID NO:220 selected from the group consisting of: