Patent ID: 6066499
Filing Date: 2000-05-23
Classification: A01N,C07D,C07K,C12N,C12P,C12R

Abstract:
A system for the creation or directed evolution of a molecule which interacts with a recognition sequence, said system comprising:(a) a population of host cells containing a growth factor, a modulation moiety and a recognition sequence;(b) a population of genes that express in said host cell multiple copies of a putative molecule or a multiplicity of different putative molecules; and(c) apparatus for selection and mutagenesis to impose selection and mutagenizing conditions on said population of host cells to select for those host cells and/or replicators having modified growth as a result of an interaction between said molecule which interacts with said recognition sequence, said modulation moiety and said recognition sequence, such that at least one replicator and/or cell in said population undergoes at least one cycle of division and/or replication under said conditions;wherein said putative molecule or multiplicity of different putative molecules evolve into said molecule which interacts with said recognition sequence.