Patent ID: 6372965
Filing Date: 2002-04-16
Classification: C12N,C12Y

Abstract:
An isolated nucleic acid fragment comprising a nucleic acid sequence encoding a plant enzyme selected from the group consisting of a delta-12 desaturase and a delta-12 hydroxylase wherein said isolated nucleic acid fragment hybridizes to one of the nucleotide sequences set forth in SEQ ID NOS:1, 3, 5, 7, 9, 11, and 15 under one of the following sets of conditions:(a) hybridization in 50 mM Tris, pH 7.6, 6Ã—SSC, 5Ã—Denhardt's, 0.5% sodium dodecyl sulfate (SDS), 100 &mgr;g denatured calf thymus DNA at 50Â° C. and wash twice with 2Ã—SSC, 0.5% SDS at room temperature for 15 min each, then wash twice with 0.2Ã—SSC, 0.5% SDS at room temperature for 15 min each and then wash twice with 0.2Ã—SSC, 0.5% SDS at 50Â° C. for 15 min each; (b) hybridization in 6Ã—SSPE, 5Ã—Denhardt's solution, 0.5% sodium dodecyl sulfate (SDS), 5% dextran sulfate, 100 &mgr;g denatured calf thymus DNA at 50Â° C. and wash twice with 2Ã—SSC, 0.5% SDS at room temperature for 15 min each, then wash twice with 0.2Ã—SSC, 0.5% SDS at room temperature for 15 min each and then wash twice with 0.2Ã—SSC, 0.5% SDS at 50Â° C. for 15 min each; or (c) hybridization in 50% formamide, 5Ã—SSPE, 1% sodium dodecyl sulfate (SDS), 1% Denhardt's Reagent, 100 &mgr;g denatured salmon sperm DNA at 42Â° C. and wash twice with 2Ã—SSPE, 0.2% SDS at 42Â° C. for 15 min each, then wash twice with 0.2Ã—SSPE, 0.2% SDS at 55Â° C. for 30 min each.