Patent ID: 7531335
Filing Date: 2009-05-12
Classification: C12N,C12P,C12Q,G01N

Abstract:
1. A process for the enzymatic production of subterminally hydroxylated aliphatic carboxylic acids, which comprises a1) culturing a recombinant microorganism which has been transformed with a vector which encompasses an expression construct comprising, under the genetic control of regulatory nucleic acid sequences, a sequence which encompasses a nucleic acid sequence encoding a modified monooxygenase having a modification in the amino acid sequence of SEQ ID NO:2, which modification consists of one functional mutation in each of amino acid sequence positions 87 and 188 and, optionally, at least one additional functional mutation in one of amino acid sequence positions 26, 47, 72, 74 and 354, wherein:  wherein the functional mutation in comparison with the wild-type enzyme, results in an altered activity or regioselectivity in the subterminal enzymatic hydroxylation of an aliphatic C a2) incubating a reaction medium containing at least one hydroxylatable C b) isolating the resulting hydroxylated products from the medium.