Patent ID: 6329186
Filing Date: 2001-12-11
Classification: C12N

Abstract:
A variant glucoamylase having an amino acid sequence that differs from the amino acid sequence of a parent glucoamylase, wherein the difference between the amino acid sequence of the variant glucoamylase and the amino acid sequence of the parent glucoamylase comprises a peptide extension linked to the N-terminal amino acid residue and wherein the peptide extension is:Asn-Val-Ile-Ser-Arg-Arg (SEQ ID NO:36), orAsn-Val-Ile-Pro-Lys-Arg (SEQ ID NO:37), orAla-Ser-Pro-Pro-Ser-Thr-Ser (SEQ ID NO:38), orPro-Leu-Ala-Leu-Ser-Asp (SEQ ID NO:41), orLeu-Gly-Val-Thr-Gly-Glu (SEQ ID NO:42), orAla-Gly-Pro-Leu-Pro-Ser-Glu (SEQ ID NO:43), orLeu-Gly-Pro-Asp (SEQ ID NO:44), orIle-Phe-Glu-Leu-Thr-Pro-Arg (SEQ ID NO:45), orIle-Ser-Asn, orMet-Asn, ora peptide of formula (I):Xaa-C-(Xaa).sub.n (I),wherein each Xaa is independently an amino acid.