Patent ID: 8158370
Filing Date: 2012-04-17
Classification: A61P,C07K,G01N

Abstract:
1. A method of detecting antibodies that specifically bind an Anaplasma phagocytophilum polypeptide or an Anaplasma platys polypeptide in a test sample, comprising: (a) contacting a purified polypeptide at least 95% identical to SEQ ID NO:10, wherein, optionally, the purified polypeptide is linked to an indicator reagent, an amino acid spacer, an amino acid linker, a signal sequence, a stop transfer sequence, a transmembrane domain, a protein purification ligand, a heterologous protein, one or more additional polypeptides comprising SEQ ID NO:1, 2, 6, 8, 9, 10,11, 12, or a combination thereof, with the test sample, under conditions that allow polypeptide/antibody complexes to form; (b) detecting the polypeptide/antibody complexes; wherein the detection of the polypeptide/antibody complexes is an indication that antibodies specific for an Anaplasma phagocytophilum polypeptide or an Anaplasma platys polypeptide are present in the test sample.