Patent ID: 6977154
Filing Date: 2005-12-20
Classification: C07K,C12N,C40B

Abstract:
1. A method for binding a DNA binding polypeptide of the Cys2 His2 zinc finger class to a DNA triplet in a target DNA sequence comprising 5-meC as the central residue in the target DNA triplet, the method comprising preparing a DNA binding polypeptide of the Cys2 His2 zinc finger class to bind to the DNA triplet, wherein binding to the 5-meC residue by an α-helical zinc finger DNA binding motif of the polypeptide is achieved by placing an Ala residue at position +3 of the α-helix of the zinc finger, and exposing the DNA binding polypeptide to the target DNA sequence, whereby the DNA binding polypeptide binds to the target DNA sequence.