Patent ID: 6592745
Filing Date: 2003-07-15
Classification: C12Q,G01N,Y10T

Abstract:
A method for determining a concentration of glucose in a sample, comprising the steps of:(a) contacting a sample with an electrochemical sensor comprising: (i) an electrode pair comprising a working electrode and a counter electrode, wherein the working electrode and counter electrode are separated by a closest distance in a range of 200 to 1000 &mgr;m; (ii) a measurement zone positioned adjacent to the working electrode and the counter electrode, wherein the measurement zone is sized to contain a volume of no more than about 1 &mgr;L of the sample; and (iii) an analyte-responsive enzyme and a diffusible redox mediator disposed in the measurement zone; (b) holding the sample within the measurement zone in a non-flowing manner; (c) generating a sensor signal at the working electrode within a measurement period of no greater than about 5 minutes, wherein a background signal that is generated by the redox mediator is no more than five times a signal generated by oxidation or reduction of 5 mM of glucose; and (d) determining the concentration of the glucose using the sensor signal.