Patent ID: 8063196
Filing Date: 2011-11-22
Classification: C12Q,Y02A

Abstract:
1. A sequence prepared from six nucleotide bases, wherein the six nucleotide bases are comprised of four natural bases selected from the group consisting of guanosine (G), cytosine (C), adenosine (A), thymidine (T), and uracil (U) and two non-natural bases selected from isoguanosine (isoG) and isocytosine (isoC), wherein for the length of the sequence, one to five of the natural bases, arranged in no specific order, are separated by isoC or isoG, wherein at least 65% of the sequence is comprised of G, C, isoC, and isoG and the sequence has a melting temperature Tm of approximately 80-85° C., wherein the number of isoG and isoC bases in the sequence is balanced and the sequence has at least one incidence of five natural bases separated by isoC or isoG.