Patent ID: 6878532
Filing Date: 2005-04-12
Classification: C12P,Y10S

Abstract:
1. A method of making phosphatidylserine (PS) comprising: producing phospholipase D (PLD) enzyme from phospholipase-producing microorganisms; combining the PLD enzyme with a source of serine and a source of phosphatidylcholine-containing phospholipid to form a reaction mixture; said phospholipid being dissolved in an organic solvent; adding an alcohol to the reaction mixture to create an aqueous/organic interphase; allowing the mixture to react for a time period sufficient to produce PS; allowing the reaction mixture to separate into an aqueous phase and an organic phase following the production of the PS; reincubating the aqueous phase with a phospholipid; separating the enzyme from the serine in the aqueous phase; and reusing the separated serine and the separated enzyme in the combination step.