Patent ID: 6046018
Filing Date: 2000-04-04
Classification: C12Q

Abstract:
An assay method of chiroinositol which comprises reacting specimen with1) a dehydrogenase, which catalyzes at least reversible reaction with a substrate of chiroinositol in the presence of a coenzyme selected from nicotinamide adenine dinucleotides (phosphate), hereinafter designates as NAD(P)s and a coenzyme selected from thio-nicotinamide adenine dinucleotides (phosphate), hereinafter designates as thio-NAD(P)s,2) A1 and3) B1to form cycling reaction of the formula ##STR6## wherein a product is a compound, from which 2 or 4 hydrogen atoms are deleted from chiroinositol, A1 is NAD(P)s or thio-NAD(P)s, A2 is a reduced form of A1, B1 is a reduced form of NAD(P)s in case of A1 being thio-NAD(P)s or a reduced form of thio-NAD(P)s in case of A1 being NAD(P)s and B2 is an oxidized form of B1, and determining an amount of converted A2 or B1 by the said reaction.