Patent ID: 8058028
Filing Date: 2011-11-15
Classification: A01K,A61K,C12N

Abstract:
1. A method of obtaining a desired gene product comprising culturing a host cell, wherein the host cell comprises a vector, and the vector comprises a polynucleotide, the polynucleotide comprising: (i) at least one extended methylation-free CpG-island comprising dual or bi-directional promoters that are divergently transcribed; (ii) an expressible gene encoding a desired gene product, wherein the expressible gene is operably-linked to the at least one CpG-island; and (iii) a promoter, operably-linked to the expressible gene, wherein said promoter is not naturally operably-linked to said CpG-island; wherein the at least one extended methylation-free CpG island facilitates reproducible activation of transcription of the expressible gene.