Patent ID: 7824852
Filing Date: 2010-11-02
Classification: C12Q

Abstract:
1. A method for detecting the methylation status of a cytosine position in genomic DNA samples, comprising: a) chemically treating a genomic DNA from a DNA sample with a reagent whereby 5-methylcytosine and cytosine react differently to said reagent, thus exhibiting different base pairing behaviors in the DNA duplex subsequent to the chemical treatment, b) amplifying the chemically treated DNA using a polymerase and at least one oligonucleotide (type A) as a primer, c) hybridizing the amplified genomic DNA to two oligonucleotides (type B) specific for DNA treated according to step (a) wherein d) elongating the one of the two oligonucleotides (type B) that has previously hybridized with its 3′-end to the position to be analyzed without mispairings by up to a maximum of ten nucleotides by means of a polymerase and a mixture of nucleotides e) comparing the signals derived from both oligonucleotides (type B) and deducing therefrom the methylation status of said cytosine position.