Patent ID: 7270977
Filing Date: 2007-09-18
Classification: C12N,C12Q,G01N

Abstract:
1. A method of identifying one or more fungicides which are capable of controlling phytopathogenic fungi which attack and damage plants, plant parts or plant products or reduce their yield or value comprising (a) contacting an inosine 5′-monophosphate dehydrogenase having the amino acid sequence of SEQ ID NO: 2 or 4 with a chemical compound or a mixture of chemical compounds, (b) comparing the ability of the inosine 5′-monophosphate dehydrogenase to catalyse the conversion of inosine 5′-monophosphate into xanthosine 5′-monophosphate in the presence of the chemical compound or the mixture of chemical compounds with the ability of an inosine 5′-monophosphate dehydrogenase to catalyse the conversion of inosine 5′-monophosphate into xanthosine 5′-monophosphate in the absence of the chemical compound or the mixture of chemical compounds, wherein the ability of the inosine 5′-monophosphate dehydrogenase to catalyze the conversion of inosine 5′ monophosphate into xanthosine 5′-monophosphate is determined by photospectrometrically monitoring the conversion of the substrate NAD+ into NADH, (c) determining if said chemical compound or said mixture of chemical compounds inhibits the ability of the inosine 5′-monophosphate dehydrogenase to catalyse the conversion of inosine 5′-monophosphate into xanthosine 5′-monophosphate, wherein said ability to inhibit the conversion of the inosine 5′-monophosphate into xanthosine 5″-monophosphate indicates that the chemical compound or the mixture of chemical compounds is a fungicide, and (d) verifying the fungicidal action of the compound or the mixture of chemical compounds by bringing it into contact with one or more phytopathogenic fungi.