Patent ID: 7576259
Filing Date: 2009-08-18
Classification: A01K,C07K,C12N

Abstract:
1. A method for making a mouse embryo homozygous for a genetic modification, comprising: (a) incorporating a female mouse ES donor cell into a first pre-morula diploid mouse host embryo, wherein the female mouse ES donor cell is heterozygous for the genetic modification and is from an inbred strain; (b) culturing the first embryo to the blastocyst stage; (c) gestating the embryo of (b) in a surrogate mother, wherein upon gestation a modified female mouse is obtained having at least 90% cellular contribution from the female mouse ES donor cell; (a′) incorporating a male mouse ES donor cell into a second pre-morula diploid mouse host embryo, wherein the male mouse ES donor cell is heterozygous for the genetic modification and is from an inbred strain; (b′) culturing the embryo of (a′) to the blastocyst stage; (c′) gestating the embryo of (b′) in a surrogate mother, wherein upon gestation a modified male mouse is obtained having at least 90% cellular contribution from the male mouse ES donor cell; and, (d) breeding the modified female mouse of (c) with the modified male mouse of (c′) to obtain a mouse embryo homozygous for the genetic modification.