Patent ID: 6511814
Filing Date: 2003-01-28
Classification: G01N,Y10S

Abstract:
A method for detecting and quantifying at least one analyte in a fluid sample suspected of containing the analyte by employing a liquid reactant that reacts with the analyte to form a detectable reaction product, by relating the analyte to the detectable reaction product of analyte and liquid reactant comprising:providing a fluid transport material that absorbs a liquid and supports capillary flow of the liquid, the fluid transport material having a first zone for the application of the fluid sample suspected of containing the analyte, and a second zone for the application of the liquid reactant; adding the fluid sample to the first zone, and the liquid reactant to the second zone, wherein the fluid sample thereafter flows in a first direction from a fluid sample edge toward the second zone, and the liquid reactant flows in a second direction opposite to that of the first direction and toward the first zone from a liquid reactant edge; whereby when the flowing fluid sample and the flowing liquid reactant meet, flow stops and the detectable reaction product is formed by a reaction between the liquid reactant and the analyte, and a stable reaction interface is formed at a juncture between and visually distinct from the fluid sample and the liquid reactant; and detecting the detectable reaction product.