Patent ID: 7541455
Filing Date: 2009-06-02
Classification: B01J,C07H,C40B

Abstract:
1. A method for conducting a bisulfite-catalyzed reaction of a compound, the compound selected from the group consisting of cytosine, cytidine, deoxycytidine, cytidine triphosphate, deoxycytidine triphosphate, cytidylate, deoxycytidylate, and a polynucleotide comprising cytidine or deoxycytidine, the method comprising the steps of: activating a C4 amino group of a cytosine ring of the compound by reacting the compound with bisulfite to form a bisulfite adduct of the compound having the structure: wherein R is H, ribosyl, 2′-deoxyribosyl, ribosyl-5′-phosphate, 2′-deoxyribosyl-5′-phosphate, ribosyl-5′-triphosphate, 2′-deoxyribosyl-5′-triphosphate, a ribosyl-5′-phosphate moiety of a polyribonucleotide or a 2′-deoxyribosyl-5′-phosphate moiety of a polydeoxyribonucleotide; and contacting the adduct with a nucleophile, thereby displacing the amino group with the nucleophile, the nucleophile selected from the group consisting of H wherein said activating step, said contacting step, or both, are conducted in the presence of microwave energy having a wavelength of between 1 mm and 1 meter and said bisulfite-catalyzed reaction is accelerated at a given temperature relative to said bisulfite-catalyzed reaction conducted at the same temperature in the absence of said microwave energy.