Patent ID: 7341855
Filing Date: 2008-03-11
Classification: C12N,C12P,C12R

Abstract:
1. A simple and efficient method of obtaining high yield of pure mannitol from a plant mangrove-associated fungus Curvularia lunata of International deposition No. MTCC 5109, said method comprising steps of: a. obtaining leaves from the plant mangrove, b. rinsing the leaves in sterile seawater and keeping them in sterile, moist culture chamber for about two weeks, c. cutting the leaves into small pieces and placing it on potato dextrose agar (PDA) plates for about 48 hours, d. transferring individual colonies aseptically into a sterile fresh PDA plate to obtain pure culture, e. maintaining the isolated fungal cultures on Potato Dextrose Agar (PDA) slants for about 7 days to obtain mycelia, f. transferring the mycelia to a fresh Potato Dextrose Broth (PDB) medium with seawater and distilled water using starch and sugar as source of carbon, g. incubating the culture at temperature ranging between 26-32° C. for about 3-6 days to obtain inoculum, h. inoculating the PDB medium having seawater and distilled water with the inoculum, i. maintaining the culture at temperature ranging between 26-47° C. and salinity ranging between 4-32 ppt with occasional stress conditions for about 17-19 days to obtain mycelial mat, j. sonicating the mat to lyse the cells, k. extracting crude from the sonicated mat using methanol repeatedly, l. concentrating the crude, m. treating the concentrated crude extract with solvents of increasing polarity, n. obtaining aqueous fraction as white powder residue after the treatment containing crude mannitol, o. purifying the crude mannitol by chromatography to obtain pure mannitol with about 75% of the total crude extract.