Patent ID: 8097439
Filing Date: 2012-01-17
Classification: C12N,C12P,Y02P

Abstract:
1. A method of producing a fatty aldehyde, the method comprising: (a) providing a bacterial host cell, said bacterial host cell engineered to express a gene encoding a polypeptide having at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 22 (carB), and comprising the phosphopantetheine attachment site sequence of SEQ ID NO: 9, wherein the polypeptide has carboxylic acid reductase activity effective to convert a fatty acid to a fatty aldehyde; and (b) culturing said engineered bacterial host cell in a culture media containing a carbohydrate carbon source under conditions effective to produce a fatty aldehyde at a yield of at least 25 mg/L in the media of the bacterial host cell culture, when cultured for 20 hours at 37° C.