Patent ID: 8758988
Filing Date: 2014-06-24
Classification: A01N

Abstract:
1. A method for cryopreserving articular cartilage using more than one cryopreserving agent (CPA), the method comprising: permeating a sample of articular cartilage with a sequence of at least two different CPAs comprising a first CPA and a second CPA, the second CPA being permeated into the sample after permeating the sample with the first CPA, to form combined CPAs having a concentration distribution within the sample; the concentration distribution of the combined CPAs being selected so that upon cooling of the sample, the combined CPAs vitrify and cryopreserve the sample; and vitrifying the sample by cooling the sample below the glass transition temperature of the permeated sample of articular cartilage in a sequence of cooling steps corresponding to the sequential addition of CPAs.