Patent ID: 8906626
Filing Date: 2014-12-09
Classification: C12Q,Y10T

Abstract:
1. A method for detecting different target nucleic acids comprising DNA in a sample, each nucleic acid comprising, from 3′ to 5′: contiguous first, second, and third target domains, the method comprising: (a) providing a sample having the different target nucleic acids comprising DNA; (b) contacting the sample with a plurality of at least 100 different probe sets to form hybridization complexes with the different target nucleic acids, wherein each probe set comprises: (c) contacting the hybridization complexes with an extension enzyme and nucleotides, wherein the first probes are extended along the second target domains of hybridization complexes formed in (b), (d) ligating the extended first probes to the second probes to form amplification templates; (e) amplifying the amplification templates with first and second primers that are complementary to the first priming sequence and the second priming sequence to produce amplicons; and (f) detecting the amplicons on the surface of a nucleic acid array that is different from the solid support that is immobilized to the hybridization complexes.