Patent ID: 7763461
Filing Date: 2010-07-27
Classification: A01K,A61K,A61P

Abstract:
1. A method of making a master cell bank of whole cell vaccines for the treatment of cancer comprising: obtaining a sample of live cells from a human tumor cell line selected from the group consisting of A549, NCI-H460, NCI H520, MCF-7, BT-20, HPAF-II, PANC-1, ASPC-1, BxPC3, IGROV, ES-2, NIH:OVCAR3, PA-1, COLO829, G-361, HCT-116, COLO205, LoVo, WiDr, DLD-1, HCT-15, SW620, SW480, SW1116, HT-29, FHC, CCD841CoN, PC-3, LNCaPFGC, MDAPca2b, and DU145, transforming said cells with a retroviral vector comprising the sequence of SEQ ID NO:1 which encodes upon expression α(1,3) galactosyltransferase, so that said α(1,3) galactosyltransferase synthesizes an α galactosyl epitope on a plurality of cell surface glycoproteins on said cells to produce a population of cells, staining the population of cells with an anti-alphaGal antibody to identify cells expressing an α galactosyl epitope; selecting from the population, by fluorescence activated cell sorting, five percent of the cells with the highest expression level of the alphaGal epitopes on their cell surface to obtain a subpopulation of cells; expanding the subpopulation of cells to generate a master cell bank of cells expressing alphaGal epitopes for the treatment of cancer; and freezing the cells.