Patent ID: 7807418
Filing Date: 2010-10-05
Classification: C07D,C07H,C07K,C12N,C12P

Abstract:
1. A method for producing a gene assembly encoding a functional hybrid type I polyketide synthase (PKS), said method comprises joining: a) a first nucleic acid molecule which encodes at least one domain of a first type I PKS, wherein said first nucleic acid molecule consists of a nucleic acid sequence encoding the loading module and the ketosynthase (KS) domain of the first adjacent extension module of said first type I PKS, wherein said loading module consists of an acyltransferase (AT) domain and an acyl carrier protein (ACP) domain; and b) a second nucleic acid molecule which encodes an extension module(s) which lacks the first ketosynthase domain of a second type I PKS; thereby forming a gene assembly encoding a functional hybrid type I PKS which produces a polyketide, wherein said first type I PKS is heterologous to said second type I PKS.