Patent ID: 7494794
Filing Date: 2009-02-24
Classification: C12N

Abstract:
1. An isolated glucose dehydrogenase (GDH) derived from filamentous fungus belonging to genus Penicillium and having the following physicochemical properties (a) to (f): (a) optimum reaction temperature: 50° C.; (b) optimum reaction pH: about 6.5; (c) temperature stability: residual activity ratio of GDH after being treated at 55° C. for 15 minutes is 90% or more and the residual activity ratio of GDH after being treated at 60° C. for 15 minutes is 40% or more; (d) pH stability: 5.0 to 8.0 (the residual activity ratio of GDH after being treated at 25° C. for 16 hours is 90% or more); (e) substrate specificity: when an action upon glucose is 100, the action upon xylose is about 10%, the action upon 2-deoxy-D-glucose is about 14%, and a reactivity to maltose, fructose, arabinose, sucrose, galactose, mannose, melezitose, sorbose, ribose, maltotriose, maltotetraose and trehalose is less than 2%; and (f) effects of chemicals: strongly inhibited by cupper, silver and cadmium and inhibited by monoiodoacetic acid, N-ethyl maleimide, hydroxylamine and sodium azide.