Patent ID: 8795981
Filing Date: 2014-08-05
Classification: G01N

Abstract:
1. A method of identifying a cell or cell colony which produces a polypeptide of interest, the method comprising: a) exposing one or more cells in a same medium to tetrahydrofolate and a marker compound comprising methotrexate which associates with a reference polypeptide comprising dihydrofolate reductase, wherein production of the polypeptide of interest by the one or more cells is linked to production of the reference polypeptide; and b) detecting association of the marker compound with the one or more cells, thereby identifying a cell or cell colony which produces the polypeptide of interest, wherein a gene encoding the reference polypeptide is amplified in the identified cell or cell colony, and wherein detecting association of the marker compound comprises (i) obtaining an image of the one or more cells and (ii) analysing the image to identify association of the marker compound with the identified cell or cell colony.