Patent ID: 7534560
Filing Date: 2009-05-19
Classification: C12Q,G01N,Y10T

Abstract:
1. A method of detecting the presence of an ion in a sample, comprising: (a) forming a mixture comprising: (i) the sample; and (ii) an aggregate, wherein the aggregate comprises a plurality of complexes, wherein each complex comprises (I) a nucleic acid enzyme; (II) particles; (III) a first oligonucleotide attached to a solid surface of the particles; (IV) a second oligonucleotide attached to the solid surface of the particles; and (V) a substrate for the nucleic acid enzyme, wherein the substrate hybridizes to the first oligonucleotide and the second oligonucleotide, wherein the nucleic acid enzyme depends on the ion to cause cleavage of the substrate and cleavage of the substrate followed by heating to 50° C. results in de-aggregation of the aggregate; and (b) detecting deaggregation of the aggregate.