Patent ID: 7238530
Filing Date: 2007-07-03
Classification: C12N,C12Q

Abstract:
1. A method for separating single stranded nucleic acid from double stranded nucleic acid, comprising the steps of: contacting a mixture comprising both single stranded nucleic acid and double stranded nucleic acid with a first liquid comprising a chaotropic agent and a nucleic acid binding solid phase, wherein the first liquid has a composition such that the double stranded nucleic acid binds to the solid phase; separating the solid phase from a supernatant containing the single stranded nucleic acid; and contacting the supernatant with a second nucleic acid binding solid phase, and a second liquid consisting essentially of material selected from the group consisting of: a) a chaotropic agent b) a chaotropic agent and a chelating agent; c) a chaotropic agent and divalent positive ions; and d) a chaotropic agent, a chelating agent and divalent positive ions, wherein the second liquid has a composition such that the resulting mixture of supernatant and second liquid allows for binding of the single stranded nucleic acid to the second solid phase.