Patent ID: 7608682
Filing Date: 2009-10-27
Classification: A61P,C07K,C12Q,G01N

Abstract:
1. An isolated conjugate comprising (a) at least (b) a protein selected from the group consisting of aprataxin, synaptogamin-like protein (SLP), high mobility group protein 17 (HMG17), PIN2-interacting protein 1 (PinX1), CBF1 interacting corepressor (CIR), high mobility group nucleosomal binding protein 3 (HMGN3), hematopoietic stem/progenitor cell 144 (HSPC144), Cullin 3, cell division cycle 6 (CDC6), and fragments and variants thereof; wherein (i) said variants comprise polypeptides of at least 50 amino acids having at least 90% sequence identity to sequences within their corresponding proteins and (ii) said fragments comprise polypeptides of at least 50 amino acids having at least 90% identity to a sequence fragment of said protein; and said conjugate is formed via N-end rule ubiquitylation of a polypeptide comprising a destabilizing N-terminal residue and an internal Lys residue.