Patent ID: 6613769
Filing Date: 2003-09-02
Classification: A61P,C07C,C07D

Abstract:
A method for determining tryptase in a biological sample, comprisingobtaining a biological sample suspected of containing tryptase, incubating said biological sample with a tryptase inhibitor, and determining any complex formed between said tryptase inhibitor and any tryptase in said biological sample, wherein said tryptase inhibitor is a compound of formula I whereinA1 and A2 are each independently selected from the group consisting of â€”Oâ€” and â€”NHâ€”C(O)â€”, A3 and A4 are each independently selected from the group consisting of â€”C(O)â€”NHâ€” and â€ƒwhere W is the group â€”C(O)â€” or a bond, A5 and A6 are each independently selected from the group consisting of â€”C(O)â€”, â€”C(O)â€”NHâ€”, â€”NHâ€”C(O)â€” and a bond, M is selected from one of the following groups: K1 is -B7-(C(O))m-B9-X1 or -B7-(C(O))m-B9-Z1-B11-X1, K2 is -B8-(C(O))p-B10-X2 or -B8-(C(O))p-B10-Z2-B12-X2, B1, B2, B3, B4, B5 and B6 are each independently selected from the group consisting of a bond and â€”CH2â€”, B7, B8, B9, B10, B11 and B12 are each independently selected from the group consisting of a bond and 1-2C-alkylene, m is 0 or 1, p is 0 or 1, X1 and X2 are each independently selected from the group consisting of amino, amidino and guanidino, and Z1 and Z2 are each independently selected from the group consisting of 1,4-phenylene, 1,3-phenylene, 1,4-cyclohexylene or 1,4-piperazinylene, wherein 24 to 40 bonds are present on a direct route between terminal nitrogen atoms, and a pharmaceutically acceptable salt of said compound wherein compounds in which at least one to twelve of the variables B1, B2, B3, B4, B5, B6, B7, B8, B9, B10, B11 or B12 is a bond resulting in the direct linkage of two heteroatoms or two carbonyl groups are excluded.