Patent ID: 6458530
Filing Date: 2002-10-01
Classification: B01J,C12Q,C40B

Abstract:
A method for selecting a set of tag nucleic acids, said method comprising:(a) providing a plurality of candidate tag nucleic acid sequences, wherein the total number of nucleotides in each tag nucleic acid sequence is identical, the number of G+C nucleotides in each tag nucleic acid sequence is identical and the overall number of A+G nucleotides in each of the tag nucleic acids sequences is even; (b) aligning a first candidate tag with each of the other candidate tags in the plurality; (c) excluding the first candidate tag from the plurality if it can be aligned with less than two errors to another candidate tag, wherein an error is either a mismatch hybridization or an overlapping nucleotide, (d) repeating (b) and (c) for each of the remaining candidate tags in the plurality, thereby providing a set of nucleic acid tags; and (e) synthesizing at least 100 members of the set of nucleic acid tags.