Patent ID: 9206234
Filing Date: 2015-12-08
Classification: A61K,A61P,C07K,Y02P

Abstract:
1. A method of making a peptide comprising the amino acid sequence WDLYFEIVW (SEQ ID NO: 322) or a variant amino acid sequence comprising one, two, three or four amino acid substitutions in WDLYFEIVW (SEQ ID NO: 322), said variant amino acid sequence comprising X1X2X3YX4EX5X6X7, wherein X1 is W, L, or P; X2 is D or S, X3 is L or F, X4 is F, Phg, L, Ebw, Pff, Thi, 1Ni, Hfe, Ece or Cha, X5 is I or F, X6 is S, V or G, and X7 is W or L (SEQ ID NO: 323), the method comprising (a) coupling the C-terminal amino acid of the peptide to a cleavable linking moiety that is bonded to a solid phase support material, wherein the alpha-amino group of the C-terminal amino acid that is to be coupled bears an Fmoc protecting group; (b) removing the Fmoc protecting group from the C-terminal amino acid that is coupled to the linking moiety; (c) successively coupling Fmoc-protected amino acids to the C-terminal amino acid, with attendant cleavage of the Fmoc protecting group prior to each successive amino acid addition, thereby producing an amino acid sequence bearing side chain protecting groups; and (d) removing the side chain protecting groups and cleaving the peptide from the solid phase support material.