Patent ID: 8143390
Filing Date: 2012-03-27
Classification: A61P,C12N

Abstract:
1. An isolated double-stranded ribonucleic acid (dsRNA) comprising a sense strand and an antisense strand, wherein the dsRNA is between 15 and 30 nucleotides in length, wherein the sense strand comprises a first sequence and wherein the antisense strand comprises a second sequence that comprises a region of complementarity which is substantially complementary to at least a part of an mRNA encoding bcl-2, wherein the region of complementarity is between 15 and 30 nucleotides in length, wherein a region of complementarity between the sense strand and the antisense strand is between 15 and 30 nucleotides in length, wherein the dsRNA consists of 2′—O- methyl modified nucleotides located only at a penultimate and an antepenultimate position of the 3′ end of the antisense strand, and wherein the dsRNA, upon contact with a cell expressing the bcl-2, is capable of inhibiting expression of the bcl-2 gene by at least 20%.