Patent ID: 6815581
Filing Date: 2004-11-09
Classification: A23B,A23L,C07K,C08B,C12N,C12P

Abstract:
An isolated DNA molecule encoding an antisense-RNA complementary to a transcript of a nucleic acid molecule encoding a protein which is present in plant cells in starch granule-bound form as well as in soluble form and that is involved in the phosphorylation of starch when expressed in plants and/or that increases the phosphorylation of glycogen when expressed in E.coli, said nucleic acid molecule selected from the group consisting of:(a) a nucleic acid molecule comprising a nucleotide sequence that encodes a protein having the amino acid sequence of SEQ ID NO: 2; (b) a nucleic avid molecule comprising the coding region of the nucleotide sequence of SEQ ID NO: 1; (c) a nucleic acid molecule that hybridizes to the nucleic acid molecule of (a) or (b) under stringent conditions wherein the nucleic acid molecule encodes a polypeptide that is present in plant cells in starch granule-bound form as well as soluble form and that is involved in the phosphorylation of starch when expressed in plants and/or that increases the phosphorylation of glycogen when expressed in E.coli; (d) a nucleic acid molecule the sequence of which is degenerate as a result of the genetic code to a nucleic acid molecule of (a), (b) or (c); and (e) a fragment or allelic variant of a nucleic acid molecule of (a), (b), (c), or (d), wherein the fragment or allelic variant encodes a polypeptide that is present in plant cells in starch granule-bound form as well as in soluble from and that is involved in the phosphorylation of starch when expressed in plants and/or that increases the phosphorylation of glycogen when expressed in E.coli, wherein said antisense-RNA is capable of inhibiting the expression of nucleic acid molecules encoding said protein when expressed in a plant cell.