Patent ID: 8663922
Filing Date: 2014-03-04
Classification: B01L,C12Q,G01N,Y10S,Y10T

Abstract:
1. A method for detecting two or more different optical emission signals emitted from each of a plurality of reaction receptacles, said method comprising, for each optical emission signal to be detected, the steps of: (a) modulating an optical excitation signal at a known excitation signal frequency, the excitation signal having a known excitation wavelength that excites an associated optical emission signal having a known emission wavelength; (b) directing the optical excitation signal at each receptacle; (c) filtering an optical signal, including any optical emission signal emitted by the contents of each receptacle, to transmit a portion of the optical signal that is at the emission wavelength; (d) detecting the portion of the optical signal transmitted in step (c); (e) determining an emission signal frequency of the optical signal detected in step (d); and (f) discarding any portion of the optical signal detected in step (d) having an emission signal frequency determined in step (e) that is inconsistent with the known excitation signal frequency to determine the portion of the optical signal detected in step (d) that corresponds to the associated optical emission signal.