Patent ID: 6048688
Filing Date: 2000-04-11
Classification: C12Q

Abstract:
A method for detecting the presence of Pseudomonas aeruginosa in a fluid sample using a PCR amplification process to amplify a segment of the P. aeruginosa exotoxin A gene sequence, said method comprising treating strands of nucleotide fragments with a first and a second oligonucleotide primer that hybridize to a region of said nucleotide fragments wherein said segment has from 400 to about 600 base pairs, wherein said first primer has the sequence 5'-ACA ACG CCC TCA GCA TCA CCA-3' (SEQ ID NO:4) and said second primer has the sequence 5'-CGG GTC GAG CAG GCA CAA C-3' (SEQ ID NO:5), amplifying said segment of the P. aeruginosa exotoxin A gene sequence, and detecting said amplified segment, thereby indicating the presence of P. aeruginosa in said fluid sample.