Patent ID: 7794942
Filing Date: 2010-09-14
Classification: C12Q

Abstract:
1. A method for producing a cell line that is more permissive for HCV replication, said method comprising the steps of: a. providing a permissive cell line that comprises a replicating HCV RNA; b. treating said cell line of (a) with an antiviral agent; c. selecting a cell line of (b) that is void of HCV RNA; d. growing the selected cell line of (c) to generate a second cell line, and e. comparing the transduction efficiency of said second cell line with the transduction efficiency of a parental cell line used to provide the permissive cell line of (a) with a same HCV RNA, thereby identifying a cell line that is more permissive for HCV replication than the parental cell line used to provide the permissive cell line of (a).