Patent ID: 8492095
Filing Date: 2013-07-23
Classification: C12Q

Abstract:
1. A method for isothermally amplifying a target nucleic acid comprising: a) hybridizing a first primer to the target nucleic acid, the first primer comprising a 5′ RNA portion that is cleavable by an agent when the first primer is hybridized to the target nucleic acid; b) extending the first primer using a polymerase; c) cleaving the 5′ portion of the first primer to leave a first product comprising a single-stranded 3′ overhang; d) hybridizing a second primer to the single-stranded 3′ overhang of the first product, wherein the second primer is a duplicate of the first primer; e) displacing the extended first primer by extending the second primer using a polymerase, such that the extended first primer is released from the target nucleic acid; f) hybridizing a third primer with the extended first primer, the third primer comprising a 5′ RNA portion that is cleavable by an agent when the third primer is hybridized to the extended first primer; and g) repeating steps (b)-(e), wherein the first primer is replaced by the third primer to produce a second product, the second primer is replaced by a fourth primer to displace an extended third primer, and the fourth primer is a duplicate of the third primer; whereby the target nucleic acid is amplified to produce amplified products.