Patent ID: 6416948
Filing Date: 2002-07-09
Classification: C07K,C12Q

Abstract:
A method for detecting a target clonotypic nucleic acid rearrangement in hematopoietic cells from a subject having, or at risk of having, a hematopoietic neoplastic disorder comprising:a) isolating a hematopoietic neoplastic cell containing the target clonotypic nucleic acid rearrangement; b) amplifying a specific segment of the target nucleic acid containing the clonotypic rearrangement from said isolated hematopoietic neoplastic cell; c) determining the sequence of the amplified segment, thereby identifying a specific clonotypic nucleic acid rearrangement; and d) quantitatively detecting the presence of said specific clonotypic nucleic acid rearrangement in a second population of isolated intact hematopoietic cells derived from a subject having, or at risk of having, a neoplastic hematopoietic disorder, such that said target clonotypic nucleic acid rearrangement in said second population of isolated hematopoietic cells is detected.