Patent ID: 6258938
Filing Date: 2001-07-10
Classification: A61K,C07K,C12N,C12Y,G01N

Abstract:
A method for isolating a vitamin K-dependent protein, which is complexed with a metal cation and exists in a stabilized conformational state, from a mixture containing said protein, said method comprising(a) providing an antibody immobilized on a solid support, said antibody being reactive with said protein complexed with a divalent or trivalent metal cation and substantially unreactive with said protein not complexed with said metal cation,(b) contacting said mixture, in the presence of said metal cation, with said immobilized antibody to bind said protein, complexed with said metal cation, to said immobilized antibody to form an immune complex, and(c) contacting said immune complex with a compound having a binding affinity for said metal cation higher than the binding affinity of said protein for said metal cation, to remove said metal cation from said protein to induce a non-stabilized conformational state of said protein and thereby release said protein from said immobilized antibody.