Patent ID: 8026078
Filing Date: 2011-09-27
Classification: C12Q,G01N

Abstract:
1. A method of measuring the amount of a glycated protein present in a sample, comprising: pretreating said sample by adding an amount of a reagent containing a degradation a fructosyl amino acid oxidase (degradation FAOD) so that a glycated free amino acid that is present in the sample as a contaminant is degraded by the by the action of said degradation FAOD and the glycated protein remains in the sample; adding an amount of a protease reagent containing a protease to the sample to degrade said glycated protein remained in the sample and produce a degradation product of the glycated protein; adding an amount of a reagent comprising a measurement a fructosyl amino acid oxidase (measurement FAOD) to the sample after adding said protease reagent to cause a redox reaction between the measurement FAOD and the degradation product of the glycated protein and to generate hydrogen peroxide; and wherein the redox reaction is conducted in the presence of a solution containing a combination of a tetrazolium compound and sodium azide, said combination of tetrazolium compound and sodium azide being added to the sample at the same time with the protease; measuring the amount of hydrogen peroxide generated by the redox reaction to determine the amount of the glycated protein present in the sample, wherein the measurement of the amount of hydrogen peroxide comprises