Patent ID: 9150841
Filing Date: 2015-10-06
Classification: C12N,C12Y

Abstract:
1. A cell comprising a first nucleic acid encoding an iduronate-2-sulfatase (I2S) protein comprising an amino acid sequence at least 90% identical to SEQ ID NO:1; and a second nucleic acid encoding a formylglycine generating enzyme (FGE) protein comprising an amino acid sequence at least 90% identical to SEQ ID NO:5, wherein the first and/or the second nucleic acid are exogenous and wherein the cell, once cultivated under a cell culture condition, produces I2S protein comprising at least about 70% conversion of the cysteine residue corresponding to Cys59 of SEQ ID NO:1 to Cα-formylglycine (FGly), and wherein the level of iduronate-2-sulfatase protein expressed by the cell is between 0.3-fold and 10-fold higher than the level of formylglycine generating enzyme protein expressed by the cell.