Patent ID: 7662791
Filing Date: 2010-02-16
Classification: A61P,C12N

Abstract:
1. A method for inhibiting the expression of a target gene through a post-transcriptional gene silencing mechanism in a cell that expresses the targeted gene, comprising the steps of: a) providing a composition comprising an mRNA-cDNA hybrid duplex prior to contacting said cell, wherein the mRNA-cDNA hybrid duplex is capable of inhibiting the expression of said targeted gene in said cell, wherein said expression is inhibited through said post-transcriptional gene silencing mechanism, wherein said targeted gene is a β-cantenin gene, and wherein said cell is in the liver or skin of a chicken embryo; and b) contacting said cell with said composition under conditions such that the expression of said gene in said cell is inhibited, wherein the mRNA is a ribonucleic acid sequence in the sense orientation of said targeted gene and the cDNA is a deoxyribonucleic acid sequence in the anti-sense orientation of said targeted gene, and wherein the mRNA-cDNA hybrid duplex forms between said mRNA and said cDNA in a complementary region containing more than 500 base pairs.