Patent ID: 7727972
Filing Date: 2010-06-01
Classification: C12Q,G01N

Abstract:
1. A method for determining absorption of cobalamin or an analogue thereof in an individual comprising the steps of: i) providing two blood samples from said individual, wherein the first sample comprises said individual's blood prior to ingestion by said individual of nonradioactive cobalamin or an analogue thereof and the second sample comprises said individual's blood after said ingestion, ii) determining the concentration of cobalamin or an analogue thereof in the first sample, iii) determining the concentration of cobalamin or an analogue thereof in the second sample by treating complexes of cobalamin or an analogue thereof bound to and one or more carrier proteins with one or more enzymes to release cobalamin or an analogue thereof from said one or more carrier proteins, and iv) determining, on the basis of comparison of said concentrations in said two samples, whether said cobalamin or analogue thereof has been absorbed.