Patent ID: 9023617
Filing Date: 2015-05-05
Classification: A61K,C12N

Abstract:
1. An expression vector comprising a gene expression cassette comprising, in 5′ to 3′ order: (a) a first nucleic acid segment comprising a first isolated HSV LAT insulator/boundary region that consists of a contiguous nucleotide sequence from about nucleotide 8365 to about nucleotide 9273 of SEQ ID NO:109, SEQ ID NO: 110, or SEQ ID NO: 111, operably positioned 5′ of a second nucleic acid segment that comprises a selected polynucleotide of interest operably linked to a first promoter that expresses the polynucleotide in a selected host cell; and (b) a third nucleic acid segment comprising a second isolated HSV LAT insulatory/boundary region that consists of a contiguous nucleotide sequence from about nucleotide 120,208 to about nucleotide 120,940 of SEQ ID NO:109, SEQ ID NO: 110, or SEQ ID NO: 111, operably positioned 3′ of the first and the second nucleic acid segments, wherein the gene expression cassette is up to about 10,000 nucleotides in length.