Patent ID: 6623983
Filing Date: 2003-09-23
Classification: B03C,G01N,Y10S

Abstract:
A method for the immobilization of magnetically labelled particulate entities on a collection surface via binding between the members of a specific binding pair, said particulate entities being present in a mixture with at least one non-magnetic substance and comprising one member of a specific binding pair present on the surface of said particulate entities, said method comprising:(a) providing a magnetic field; (b) disposing said collection surface in said magnetic field, at least a portion of said collection surface, having affixed thereto the other member of said specific binding pair; (c) adhering said magnetically labelled particulate entities to said collection surface under the influence of said magnetic field; (d) subjecting said adhered magnetically labelled particulate entities to conditions promoting reaction between said specific binding pair members, said particulate entities becoming specifically bound directly to said collection surface, without specific binding between said collection surface and said magnetic label; (e) removing said collection surface from said magnetic field; and (f) in the absence of said magnetic field, removing any excess magnetically labelled particulate entities and non-magnetic substance present on said collection surface to yield said magnetically labelled particulate entities immobilized on said collection surface.