Patent ID: 6271345
Filing Date: 2001-08-07
Classification: C07B,C07C,C12P,C40B,Y10S

Abstract:
A composition comprising a solid phase bound to an enzyme cleavable linker on which synthesis of an organic product takes place;said linker having a recognition site for a hydrolytic enzyme;said linker having a functional group to which an organic product is bound when synthesis of the product takes place;wherein said linker is fragmented upon reaction with the hydrolytic enzyme in such a way that no parts of the linker remain in the synthesized product;wherein the enzyme recognition site is different from a site where fragmentation occurs to liberate the synthesized product from the linker; andwherein said composition has the formula I, ##STR14##whereinP is a solid phase;S is a spacer with the length equivalent to 1 to 30 methylene groups linked to said solid phase via a linkage selected from the group consisting of ester, ether, amide, sulfide and phosphate;R is hydrogen or an inert radical, or two adjacent inert radicals R which together form an aromatic, heteroaromatic or aliphatic ring;n is 1or 2;R.sup.1 is substituted or unsubstituted C.sub.1 -C.sub.20 -alkyl, C.sub.3 -C.sub.20 -alkenyl, C.sub.3 -C.sub.6 -alkynyl, C.sub.1 -C.sub.20 -alkylcarbonyl, C.sub.1 -C.sub.20 -alkylphosphoryl, C.sub.3 -C.sub.20 -alkenylcarbonyl, C.sub.3 -C.sub.e -alkynylcarbonyl, C.sub.3 -C.sub.20 -alkenylphosphoryl, C.sub.3 -C.sub.6 -alkynylphosphoryl, C.sub.3 -C.sub.20 -cycloalkyl, C.sub.3 -C.sub.20 -cycloalkylcarbonyl, C.sub.3 -C.sub.20 -cycloalkylphosphoryl, aryl, arylcarbonyl, hetaryl, hetarylcarbonyl, hetarylphosphoryl, glycosyl, substituted or unsubstituted amino acids or peptides; andR.sup.2 is a nucleofugic group.