Patent ID: 8415099
Filing Date: 2013-04-09
Classification: C12Q

Abstract:
1. A method of identifying a first nucleotide at a detection position of a target sequence comprising a plurality of detection positions, said method comprising: (a) providing a surface comprising a plurality of concatemers, wherein each concatemer comprises a plurality of monomers and each monomer comprises: b) hybridizing a first anchor probe to said first anchor site; c) hybridizing a second anchor probe to said second anchor site, wherein said second anchor probe also hybridizes to sequences outside said second anchor site; d) hybridizing at least a first sequencing probe to said first target domain, wherein said first sequencing probe comprises: under conditions wherein if said unique nucleotide is complementary to a first nucleotide in said first set of target detection positions, said sequencing probe hybridizes to said concatemer; and e) ligating said first anchor probe, said second anchor probe, and said sequencing probe to form a probe ligation products; and; f) detecting said probe ligation product, thereby identifying said first nucleotide.