Patent ID: 8338112
Filing Date: 2012-12-25
Classification: A61P,C07K,G01N

Abstract:
1. A method for detecting human sonic hedgehog expression in tissue and/or determining if the polypeptide is overexpressed in said tissue comprising the steps of: (a) removing a tissue sample from a tumor in a patient afflicted with the tumor; (b) contacting the tissue with an anti-human sonic hedgehog antibody comprising a heavy chain and a light chain, wherein the heavy chain comprises an HCHVR1 comprising the amino acid sequence of SEQ ID NO:2, an HCHVR2 comprising the amino acid sequence of SEQ ID NO:4 and an HCHVR3 comprising the amino acid sequence of SEQ ID NO:6 and the light chain comprising an LCHVR1 comprising the amino acid sequence of SEQ ID NO: 10, an LCHVR2 comprising the amino acid sequence of SEQ ID NO: 12 and an LCHVR3 comprising the amino acid sequence of SEQ ID NO: 14, wherein said anti-hedgehog antibody binds to the polypeptide at an epitope within the region of amino acid residues 75-96, (c) measuring the extent of binding; and (d) determining if human sonic hedgehog is overexpressed in said tissue, compared to normal tissue.