Patent ID: 6586394
Filing Date: 2003-07-01
Classification: A61K,C07K,G01N

Abstract:
A homogeneous polypeptide composition having a molecular weight of 25(26) kDa as determined by SDS-PAGE and having the property of inhibiting growth of a mink lung cell line, CCL64 (ATCC Accession Number PTA-3450) wherein said composition is prepared by a process comprising:(a) obtaining an acidified ethanol extract of human umbilical cord; (b) hydrophobic interaction chromatography of said extract; (c) reverse phase high pressure liquid chromatography on a C18 column using an acetonitrile gradient; (d) rechromatography by reverse phase high pressure liquid chromatography on a CN column using a 2-propanol gradient; (e) recovery of fractions from step (d) having the property of inhibiting growth of a mink lung cell line, CCL64 (ATCC Accession Number PTA-3450); and (f) subjecting the fractions so recovered to SDS-PAGE.