Patent ID: 6040160
Filing Date: 2000-03-21
Classification: C12N,C12P,C12Y

Abstract:
An isolated DNA coding for a dihydrodipicolinate synthase originating from a bacterium belonging to the genus Escherichia, wherein the dihydrodipicolinate synthase has a mutation which desensitizes feedback inhibition by L-lysine, wherein the mutation is selected from the group consisting of(a) a mutation to replace the alanine residue at the 81st position as counted from the N-terminal in the amino acid sequence of the dihydrodipicolinate synthase of SEQ ID NO: 4 with another amino acid residue,(b) a mutation to replace the histidine residue at the 118th position as counted from the N-terminal in the amino acid sequence of the dihydrodipicolinate synthase of SEQ ID NO: 4 with another amino acid residue, and(c) a mutation to replace the alanine residue at the 81st position as counted from the N-terminal in the amino acid sequence of the dihydrodipicolinate synthase of SEQ ID NO: 4 with another amino acid residue and replace the histidine residue at the 118th position as counted from the N-terminal in the amino acid sequence of the dihydrodipicolinate synthase of SEQ ID NO: 4 with another amino acid residue,(d) a mutation to replace the alanine residue corresponding to the 81st position as counted from the N-terminal in the amino acid sequence of the dihydrodipicolinate synthase of SEQ ID NO: 4 with another amino acid residue,(e) a mutation to replace the histidine residue corresponding to the 118th position as counted from the N-terminal in the amino acid sequence of the dihydrodipicolinate synthase of SEQ ID NO: 4 with another amino acid residue, and(f) a mutation to replace the alanine residue corresponding to the 81st position as counted from the N-terminal in the amino acid sequence of the dihydrodipicolinate synthase of SEQ ID NO: 4 with another amino acid residue and replace the histidine residue corresponding to the 118th position as counted from the N-terminal in the amino acid sequence of the dihydrodipicolinate synthase of SEQ ID NO: 4 with another amino acid residue.