Patent ID: 8771985
Filing Date: 2014-07-08
Classification: A01K,A61K,A61P,C07K,C12N

Abstract:
1. An isolated fusion protein comprising a nuclease domain and an engineered zinc finger DNA-binding domain, wherein the engineered zinc finger DNA-binding domain binds to a target site in a rat Rosa gene and the engineered zinc finger DNA-binding domain comprises four, five or six zinc finger DNA recognition regions designated and ordered F1 to F4, F1 to F5 or F1 to F6 from N-terminus to C-terminus and further wherein the engineered zinc finger DNA-binding domain comprises the zinc finger DNA recognition regions selected from the group consisting of: (i) F1: DRSDLSR (SEQ ID NO:38); (ii) F1: QSDHLTK (SEQ ID NO:43); (iii) F1: RSDHLSE (SEQ ID NO:48); (iv) F1: QSGDLTR (SEQ ID NO:17); (v) F1: RSANLTR(SEQ ID NO:42); (vi) F1: RSDHLST (SEQ ID NO:50); (vii) F1: QSGDLTR (SEQ ID NO:17); (viii) F1: DRSDLSR (SEQ ID NO:38); (ix) F1: QSGDLTR (SEQ ID NO:17); (x) F1: TSGNLTR(SEQ ID NO:60); (xi) F1: RSDNLSE (SEQ ID NO:21); (xii) F1: TSGNLTR (SEQ ID NO:60); (xiii) F1: QRSNLVR (SEQ ID NO:65); (xiv) F1: RSDVLSE (SEQ ID NO:64); (xv) F1: RSDHLSA (SEQ ID NO:72); (xvi) F1: NNRDLIN (SEQ ID NO:74);