Patent ID: 8268598
Filing Date: 2012-09-18
Classification: C12N,C12P

Abstract:
1. An isolated nucleic acid molecule comprising a nucleotide sequence encoding a Euglena gracilis delta-5 desaturase mutant polypeptide, wherein the mutant polypeptide comprises an amino acid sequence that has at least 95% amino acid sequence identity with the amino acid sequence of SEQ ID NO:8, wherein the amino acid sequence of the mutant polypeptide has Gly or His at the position corresponding to amino acid 34 of SEQ ID NO:8, and wherein said mutant polypeptide has delta-5 desaturase enzymatic activity and has a dihomo-γ-linolenic acid to arachidonic acid conversion efficiency that is greater than the dihomo-γ-linolenic acid to arachidonic acid conversion efficiency of a wildtype