Patent ID: 6291162
Filing Date: 2001-09-18
Classification: C07D,C12N,C12P,C12Q,G01N,Y10S

Abstract:
A method of determining the amount of beta-lactamase activity in a cell, comprising the steps of:a) providing a mammalian host cell transfected with a recombinant nucleic acid molecule comprising an expression control sequence adapted for function in a mammalian cell operatively linked to a nucleic acid sequence coding for the expression of a cytosolic form of beta-lactamase,wherein said nucleotide sequence encoding a cytosolic form of beta-lactamase lacks a nucleotide sequence encoding an amino acid sequence for secretion,b) contacting a sample comprising said mammalian host cell or an extract of said mammalian host cell with a substrate for beta-lactamase, andc) determining the amount of said substrate for beta-lactamase cleaved, whereby the amount of said substrate for beta-lactamase cleaved is related to the amount of beta-lactamase activity.