Patent ID: 8632972
Filing Date: 2014-01-21
Classification: A61K,B82Y,C07K

Abstract:
1. An in vitro method of identifying a protein that binds to a predetermined RNA sequence in a cell, said method comprising the steps of: a) providing a membrane permeable construct to a cell under conditions suitable for transport of said construct across the membrane of said cell, wherein said construct comprises: b) incubating said construct with said cell under conditions suitable to allow said construct to bind with an untranslated region of an intracellular polynucleotide comprising a predetermined target mRNA sequence through a Watson/Crick base-pair binding between said nucleic acid analog and said untranslated region of said target mRNA sequence to form a construct-polynucleotide complex in said cell; c) activating said photoreactive moiety of said construct, thereby covalently cross-linking said nucleic acid analog with a nearby RNA binding protein that binds to said predetermined target mRNA sequence; d) isolating said crosslinked nucleic acid analog-protein from said cell following step c); and e) identifying said protein crosslinked to said nucleic acid analog; thereby identifying a protein that binds to a predetermined target RNA sequence.