Patent ID: 8071281
Filing Date: 2011-12-06
Classification: A01N

Abstract:
1. A method for thawing at least one frozen or vitrified embryo specimen or specimens which is contained in liquid nitrogen (LN), said method comprising the sequential steps of: a) removing a frozen or vitrified embryo specimen or specimens from said LN and placing said specimen or specimens in a first solution comprising a saline medium and a first amount of sucrose as an energy source; b) removing said specimen or specimens from said first solution and placing said specimen or specimens in a second solution comprising a saline medium and a second amount of sucrose which is less than said first amount; c) removing said specimen or specimens from said second solution and placing said specimen or specimens in a third solution comprising a saline medium and a third amount of sucrose which is less than said second amount; and d) removing said specimen or specimens from said third solution and placing said specimen or specimens in a rejuvenation medium comprising effective amounts of glucose, pyruvates, amino acids, vitamins K5 and C, antioxidants, and fatty acids sufficient to supply the specimen or specimens with the chemical ingredients and uptake requirements required to recover and prosper during and after the cryopreservation process; said rejuvenation medium being operative to supply nutrients which will replenish depletion and damage to the specimen or specimens and its or their mitochondria, spindles and structural features.