Patent ID: 9102914
Filing Date: 2015-08-11
Classification: C12N

Abstract:
1. A method for creating lineage-specific stem cells, comprising: a) seeding human embryonic stem cells (hESCs) into a trophoblast-containing three-dimensional matrix; b) growing an embryoid body from the human embryonic stem cells within the trophoblast-containing matrix; c) transferring the trophoblast-matrix containing the embryoid body from step (b) into a new culture medium that contains at least one differentiation agent that causes the ES cells to become committed lineage-specific stem cells; and d) transferring the trophoblast-matrix containing the embryoid body from step (c) into a new culture medium that contains at least one chemoattractant, wherein at least one committed lineage-specific stem cell migrates out of the embryoid body and the trophoblast-containing matrix in response to the chemoattractant.