Patent ID: 8669073
Filing Date: 2014-03-11
Classification: C12N,C12P

Abstract:
1. A composition comprising an orthogonal aminoacyl-tRNA synthetase (O-RS), wherein the O-RS preferentially aminoacylates an O-tRNA with a homoglutamine; wherein the O-RS comprises at least 95% identity to PhΔAD (SEQ ID NO:28) or to PhΔAD (SEQ ID NO:28) having an I41 and/or S268 mutation; wherein the O-tRNA comprises a CU(X) wherein the O-RS preferentially aminoacylates the O-tRNA with an efficiency at least 50% that of an O-RS corresponding to an I41 or an S268 mutation of PhΔAD (SEQ ID NO: 28) in aminoacylating an O-tRNA corresponding to SEQ ID NO: 26.