Patent ID: 6303308
Filing Date: 2001-10-16
Classification: C12N,C12Q

Abstract:
A cloning vector comprising:(a) a cloning site which permits the cloning of a nucleic acid in a defined orientation;(b) at least one cleavage site located adjacent to said cloning site, said cleavage site being rarely-occurring in nucleic acids, wherein said cleavage site is recognized by a restriction endonuclease selected from AscI, BaeI, FseI, NotI, PmeI, PpuMI, RsrII, SanDI, SapI, SexAI, SfiI, SgfI, SgrAI, SrfI, Sse8387I, SwaI, I-CeuI, PI-PspI, I-PpoI, PI-TliI, and PI-Scel, or is recognized by a restriction endonuclease with a recognition sequence of not less than 8 nucleotides that includes a CG combination;(c) a short region having several different cleavage sites which are frequently-occurring in nucleic acids, said short region being positioned on the side of said cleavage site (b) opposite to said cloning site, wherein said short region is shorter than about 100 nucleotides; and(d) a long region located on the side of said cloning site opposite to said cleavage site (b), wherein said long region and the region between said cloning site and said cleavage site (b) contain neither said cloning site nor at least three of said frequently-occurring cleavage sites, wherein said long region is longer than at least 500 nucleotides.