Patent ID: 9018364
Filing Date: 2015-04-28
Classification: C12N

Abstract:
1. An excisable nucleic acid construct comprising, in a 5′ to 3′ orientation: (a) a first genomic integration sequence; (b) a first tandem repeat nucleic acid consisting of 18 to 200 nucleotide base pairs; (c) a first homing endonuclease recognition site; (d) a target nucleic acid; (e) a second homing endonuclease recognition site; (f) a second tandem repeat nucleic acid consisting of 18 to 200 nucleotide base pairs; and (g) a second genomic integration sequence, wherein each of the first and second genomic integration sequences is of sufficient length and identity to initiate homologous recombination with a yeast genomic locus, wherein the first tandem repeat nucleic acid and the second tandem repeat nucleic acid share identical sequences, and wherein the first homing endonuclease recognition site and the second homing endonuclease recognition site share identical sequences.