Patent ID: 6794170
Filing Date: 2004-09-21
Classification: C12N

Abstract:
A process for halogenation, which comprises halogenating a chemical compound of the general structure I: where:R1=hydrogen, hydroxyl, substituted or unsubstituted, branched or unbranched C1-C10-alkyl, C2-C10-alkenyl, C1-C10-alkoxy-, C2-C10-alkenyloxy-, R6R7Nâ€”, R2=hydrogen, hydroxyl, substituted or unsubstituted, branched or unbranched C1-C10-alkyl, C1-C10-alkoxy, C2-C10-alkenyl, C2-C10-alkenyloxy, R6R7Nâ€”; R3=hydrogen, hydroxyl, substituted or unsubstituted, branched or unbranched C1-C10-alkyl, C1-C10-alkoxy, C2-C10-alkenyl, C2-C10-alkenyloxy, C1-C10-alkylcarbonyl, C2-C10-alkenylcarbonyl, aryl, hetaryl, R4 and R5 independently of one another hydrogen, hydroxyl, halogen, nitro, cyano, substituted or unsubstituted, branched or unbranched C1-C10-alkyl, C1-C10-alkoxy, C2-C10-alkenyl, C2-C10-alkenyloxy, substituted or unsubstituted C3-C10-cycloalkyl, aryl, hetaryl, R6R7Nâ€”, and where two radicals R4 and R5 on adjacent carbon atoms may together form another substituted or unsubstituted aromatic, saturated or partially saturated ring with 5 to 6 atoms in the ring which may contain one or more heteroatoms such as O, N or S; R6 and R7 independently of one another hydrogen or substituted or unsubstituted, branched or unbranched C1-C10-alkyl; R8=substituted or unsubstituted, branched or unbranched C1-C10-alkyl, C2-C10-alkenyl, substituted or unsubstituted aryl; R9=substituted or unsubstituted, branched or unbranched C1-C10-alkyl, C2-C10-alkenyl, substituted or unsubstituted aryl, hetaryl; X=â€”CHâ€” or N; Y=O or N; m=0 or 1; n=0, 1, 2 or 3; and p=0 or 1, in the presence of a halogenase, where the halogenase is encoded by(a) the sequence specified in SEQ ID NO:1, (b) a sequence derived from SEQ ID NO:1 on the basis of the degeneracy of the genetic code, or is (c) a nucleic acid sequence which codes for a fragment of (a), said fragment having halogenase activity or (d) a sequence which has more than 95% identity or more than 97% similarity with the sequence specified under (a).