Patent ID: 6525243
Filing Date: 2003-02-25
Classification: A61K,C12N

Abstract:
A method of reconstructing an embryo of a non-human mammal comprising:(a) transferring the nucleus of a donor diploid cell in the G0 phase of the cell cycle into an unactivated, enucleated metaphase II oocyte to form a reconstructed embryo, without concomitantly activating the oocyte thereby to form a reconstructed embryo, wherein the donor cell and the oocyte are from the same non-human mammalian species; (b) maintaining the reconstructed embryo without activation for a period of time such that the resultant embryo subsequently can develop to term; and (c) activating the reconstructed embryo; wherein at least one microtubule stabilizer is added to the reconstructed embryo before activation to maintain correct ploidy.