Patent ID: 7368242
Filing Date: 2008-05-06
Classification: C12Q

Abstract:
1. A kit of nucleic acid standards for determining the presence in a probe mixture of selected probes specific for interfering polymorphic loci, the kit comprising: a plurality of nucleic acid standards provided in pairwise mixtures corresponding to different homozygous and heterozygous combinations of non-interfering polymorphic loci and different diploid combinations of haplotypes of interfering polymorphic loci, wherein each nucleic acid standard comprises a double stranded nucleic acid plasmid containing a nucleic acid sequence for at least one haplotype of the interfering polymorphic loci, wherein each nucleic acid standard is capable of replication and wherein there is at least one nucleic acid standard having a nucleic acid sequence complementary with each probe of the probe mixture; and instructions that set forth a protocol comprising the steps of: hybridizing under stringent hybridization conditions each of said pairwise mixtures of said nucleic acid standards with a mixture of probes, such that the mixture of probes comprises probes specific for at least one of said nucleic acid standards in said pairwise mixture; and detecting the presence of probes in the mixture of probes that form stable duplexes with at least one of said nucleic acid standards of said pairwise mixture to determine whether such probes are present in the mixture.