Patent ID: 8269061
Filing Date: 2012-09-18
Classification: A01H,C12N

Abstract:
1. A method of selecting for haploid maize embryos comprising: a) pollinating a first maize plant with the pollen from a maize haploid inducer line to produce embryos wherein said maize haploid inducer line comprises an embryo expressed lethal polynucleotide operably linked to an embryo-preferred promoter, a non-transmission pollen polynucleotide operably linked to a pollen-specific promoter, and an embryo expressed inhibitor polynucleotide that prevents the lethality of said embryo expressed lethal polynucleotide wherein said non-transmission pollen polynucleotide and said embryo expressed inhibitor polynucleotide are linked so that they will segregate together; b) producing haploid embryos and non-viable diploid embryos; and c) selecting haploid maize embryos; further wherein said embryo expressed lethal polynucleotide is barnase and said embryo expressed inhibitor polynucleotide is barstar.