Patent ID: 6815210
Filing Date: 2004-11-09
Classification: G01N

Abstract:
In an assay for the determination of protein in an aqueous test fluid which comprises combining the test fluid with (a) a buffer selected from the group consisting of citrulline, cyanoacetic acid, methyl phosphonic acid, saccharin, or combinations thereof, the buffer is added in sufficient quantity to maintain the pH of the assay including the test fluid at a selected target pH range within a range of from about 2.0 to about 3.0 and (b) a dye having a pKa which enables it to operate as a protein indicator at the target pH range and whose affinity for protein is such that it will provide a detectable response in the presence of greater than about 15 mg/dL protein to thereby render the assay suitable for the detection of total protein in the test fluid.