Patent ID: 8008019
Filing Date: 2011-08-30
Classification: C12Q

Abstract:
1. A method for evaluating the length of a trinucleotide repeat region on an X chromosome, said method comprising: (a) amplifying a trinucleotide repeat region on the X chromosome with a primer pair, each primer of the primer pair comprising a target specific sequence, a differentiating tag sequence 5′ of the target specific sequence and a blocker between the target specific sequence and the differentiating tag sequence to produce a dual-tagged amplification product; (b) labeling the dual-tagged amplification product; (c) digesting the dual-tagged amplification product with a restriction enzyme to produce a trinucleotide repeat region fragment and an internal control fragment; (d) hybridizing the trinucleotide repeat region fragment to a first capture complex comprising an anti-tag sequence and a solid support, and hybridizing the internal control fragment to a second capture complex comprising an anti-tag sequence and a solid support; (e) detecting a signal produced by the label on the immobilized trinucleotide repeat region fragment and a signal produced by the label on the internal control fragment; and (f) comparing the intensity of the signal of the trinucleotide repeat region fragment and the signal of the internal control fragment to evaluate the length of the trinucleotide repeat region on the X chromosome.