Patent ID: 8969041
Filing Date: 2015-03-03
Classification: C07K,C12N

Abstract:
1. A method for the production of blood coagulation factor VIII (FVIII) protein, comprising: obtaining FVIIIDB-containing virus particles which comprise GFP protein; transducing human Sk-Hep-1 cells with the FVIIIDB-containing virus particles to form Sk-HEP-FVIIIDB cells; culturing the SK-HEP-FVIIIDB cells in suspension using microcarriers under appropriate conditions; purifying the FVIII protein from cell culture media; and subjecting the FVIII protein to heat treatment for viral inactivation, wherein the culturing step produces up to 1.2 to 1.4 units of FVIIIr/ml.