Patent ID: 6566068
Filing Date: 2003-05-20
Classification: C07H,C12Q

Abstract:
A process for detecting the presence of a nucleic acid sequence of interest comprising:(a) providing: (i) an oligo- or polynucleotide which is specifically hybridizable with said nucleic acid sequence of interest, said oligo or polynucleotide comprising at least one lanthanide metal or at least one fluorophore, each acting as either an energy donor or an energy acceptor; (ii) a solid matrix having at least one surface to which is fixed or immobilized a first intercalator capable of capturing double-stranded nucleic acid, and (iii) a second intercalator, which may or may not comprise at least one fluorophore, said second intercalator or said fluorophore, each acting as either an energy donor or an energy acceptor; (b) forming a complex comprising: said oligo- or polynucleotide (i), said solid matrix (ii), said second intercalator (iii), and said nucleic acid sequence of interest if contained in said sample; and (c) detecting the presence of said nucleic acid sequence of interest by detecting any energy emitted from said energy acceptor.