Patent ID: 8609934
Filing Date: 2013-12-17
Classification: C12N

Abstract:
1. A method of in vivo assembling of a recombinant transcription unit for gene suppression, said method comprising introducing into a chromosome in a cell a first segment of DNA and a distinct second segment of DNA that are not in operable linkage, each comprising a part of a recombinant transcription unit for gene suppression; wherein said first segment of DNA comprises, between left and right T-DNA borders, a promoter DNA and first segment transcribable DNA from at least one gene targeted for suppression in either a sense or an anti-sense orientation; wherein said second segment of DNA comprises, between left and right T-DNA borders, second segment transcribable DNA that has at least a part of said first segment transcribable DNA in either an antisense or a sense orientation and that does not include an operable promoter; wherein, when said first and second segments of DNA are randomly inserted in tandem in right border to right border configuration into a chromosome in a cell and assembled in vivo to form a transcription unit comprising in 5′ to 3′ order said first segment promoter, said first segment transcribable DNA in sense or anti-sense orientation, and said second segment transcribable DNA in the orientation that is opposite of the orientation of the first segment transcribable DNA, wherein RNA transcribed from said in vivo assembled transcription unit is capable of forming double-stranded RNA; and selecting a transgenic cell in which said double-stranded RNA is produced.