Patent ID: 9017958
Filing Date: 2015-04-28
Classification: G01N

Abstract:
1. A method of detecting heparin-induced thrombocytopenia (HIT) antibodies in a patient comprising: a) obtaining a serum or plasma sample from a patient suspected of having HIT; b) obtaining a solid surface to which a PF4/heparin complex has been bound; c) contacting the patient sample to the bound PF4/heparin complex, wherein HIT antibodies within the patient sample bind to the complex; d) contacting the bound HIT antibodies with at least a first and a second labeled secondary antibody, wherein the secondary antibody binds to the HIT antibodies bound to the complex and wherein the first antibody and the second antibody are specific for different isotypes selected from the group consisting of anti-Ig, anti-IgG, anti-IgA, and anti-IgM; and e) detecting the presence of HIT antibody isotypes based on the presence or absence of the label.