Patent ID: 8137963
Filing Date: 2012-03-20
Classification: C12N

Abstract:
1. A vector for amplifying a target gene in a mammalian cell, the vector including: an amplification-activating fragment not being a full-length mammalian replication initiation region but being a partial fragment of the mammalian replication initiation region, and having a gene amplification activity site; and a mammalian nuclear matrix attachment region, the amplification-activating fragment being a polynucleotide selected from the group consisting of: (a) a polynucleotide not being a full length replication initiation region of a c-myc locus but being a partial fragment of the replication initiation region of the c-myc locus, containing at least a polynucleotide having the base sequence shown in SEQ ID NO: 83, and being not shorter than 0.5 kbp but not longer than 2.0 kbp; (b) a polynucleotide not being a full-length replication initiation region of a c-myc locus but being a partial fragment of the replication initiation region of the c-myc locus, containing at least a polynucleotide having the base sequence shown in SEQ ID NO: 85, and being not shorter than 0.5 kbp but not longer than 2.0 kbp; (c) a polynucleotide not being a full-length replication initiation region of a c-myc locus but being a partial fragment of the replication initiation region of the c-myc locus, containing at least a polynucleotide having the base sequence shown in SEQ ID NO: 94, and being not shorter than 0.5 kbp but not longer than 2.0 kbp; (d) a polynucleotide not being a full-length replication initiation region of a dihydrofolate reductase locus but being a partial fragment of the replication initiation region of the dihydrofolate reductase locus, containing at least a polynucleotide having the base sequence shown in SEQ ID NO: 99, and being not shorter than 1.7 kbp but not longer than 3.5 kbp; and (e) a polynucleotide not being a full-length replication initiation region of a dihydrofolate reductase locus but being a partial fragment of the replication initiation region of the dihydrofolate reductase locus, containing at least a polynucleotide having the base sequence shown in SEQ ID NO: 102, and being not shorter than 1.7 kbp but not longer than 3.5 kbp.