Patent ID: 6455315
Filing Date: 2002-09-24
Classification: C07K,C12N

Abstract:
A method to combine multiple transfer cassettes at one location in a genome of a plant cell, said method comprising:a) stably incorporating into the genome of the plant cell, a target site comprising at least a first and a second non-identical recombination site; b) introducing into the plant cell a first transfer cassette comprising in the following order at least the first, a third, and the second, non-identical recombination sites, wherein the first and the third non-identical recombination sites of the first transfer cassette flank a first nucleotide sequence of interest; c) providing a first recombinase that recognizes and implements recombination at the first and the second non-identical recombination sites; d) introducing into the plant cell a second transfer cassette comprising at least the second and the third non-identical recombination sites, wherein the second and the third non-identical recombination sites of the second transfer cassette flank a second nucleotide sequence of interest; and, e) providing a second recombinase that recognizes and implements recombination at the second and the third non-identical recombination sites.