Patent ID: 6872524
Filing Date: 2005-03-29
Classification: C12Q

Abstract:
1. A method of detecting, and optionally selecting, a DNA sequence, wherein the DNA sequence to be detected possesses a stable expression-enhancing quality, which method comprises the steps of: 1) cloning DNA fragments into vectors at a location between i) a DNA sequence comprising a binding site for a repressor protein, which repressor protein is involved in the induction of gene-transcription repressing chromatin, and ii) a reporter gene comprising a promoter, resulting in a variety of fragment-comprising vectors; 2) introducing the vectors into host cells; and 3) subjecting the host cells to a selection step in order to identify a DNA sequence with a stable expression-enhancing quality; wherein the binding site is a DNA sequence that is recognized by a repressor protein comprising a first part recognizing said binding site and a second part inducing formation of chromatin in which transcription is repressed, which repressor protein is present in the the host cells.