Patent ID: 7598049
Filing Date: 2009-10-06
Classification: G01N

Abstract:
1. A method of aiding diagnosis of Alzheimer's disease (“AD”), comprising comparing normalized measured levels of at least forty-six AD diagnosis biomarkers in a blood sample from a human individual seeking a diagnosis for AD to reference levels for the at least forty-six biomarkers in the blood sample, wherein the human individual has a Mini Mental State Exam (MMSE) score of 14-26, wherein the reference levels are obtained from normalized measured values of the at least forty-six biomarkers from samples in the blood of human individuals without AD, wherein the at least forty-six AD diagnosis biomarkers comprise: GCSF (granulocyte-colony stimulating factor); IFN-g (interferon-gamma); IGFBP-1 (insulin-like growth factor binding protein 1); BMP-6 (bone morphogenetic protein 6); BMP-4 (bone morphogenetic protein 4); Eotaxin-2; IGFBP-2 (insulin-like growth factor binding protein 2); TARC (thymus and activation-regulated chemokine); RANTES; ANG (angiogenin); PARC (pulmonary and activation-regulated chemokine); Acrp30 (adipocyte complement-related protein of 30 kDa); AgRP(ART) (agouti-related protein (agouti-related transcript)); TIMP-1 (tissue inhibitor of metalloproteinase 1); TIMP-2 (tissue inhibitor of metalloproteinase 2); ICAM-1 (intercellular adhesion molecule 1); TRAIL R3 (tumor necrosis factor-related apoptosis-inducing ligand receptor 3); uPAR (urokinase-type plasminogen activator receptor); IGFBP-4 (insulin-like growth factor binding protein 4); LEPTIN(OB); PDGF-BB (platelet-derived growth factor BB); EGF (epidermal growth factor); BDNF (brain-derived neurotrophic factor); NT-3 (neurotrophin 3); NAP-2(neutrophil-activating peptide 2); IL-1ra (interleukin 1 receptor antagonist); MSP-a (macrophage stimulating protein alpha); SCF (stem cell factor); TGF-b3 (transforming growth factor, beta 3); TNF-b (tumor necrosis factor beta); MIP-1d; IL-3 (interleukin 3); FGF-6 (fibroblast growth factor 6); IL-6 R (interleukin-6 receptor); sTNF RII (soluble tumor necrosis factor receptor II); AXL; bFGF (basic fibroblast growth factor); FGF-4 (fibroblast growth factor 4); CNTF (ciliary neurotrophic factor); MCP-1(monocyte chemoattractant protein 1); MIP-1b (macrophage inflammatory protein-1beta); TPO (thrombopoietin); VEGF-B (vascular endothelial growth factor B); IL-8 (interleukin 8); FAS; and EGF-R (epidermal growth factor receptor), whereby the diagnosis of AD is aided by determining a difference between the normalized measured levels of the at least forty-six AD diagnosis biomarkers to the reference levels of the at least forty-six biomarkers from non-AD samples wherein the difference meets or exceeds a statistically significant difference between normalized measured values of the at least forty-six AD diagnosis biomarkers in the blood samples from individuals without AD and individuals with AD, wherein the statistically significant difference indicates a diagnosis of AD.