Patent ID: 7033782
Filing Date: 2006-04-25
Classification: A61K,C07K,C12N

Abstract:
1. A method for producing a protein from an endogenous gene in a cell comprising: (1) introducing a genetically engineered vector comprising a transcriptional regulatory sequence operably linked to an unpaired splice donor sequence into a purified eukaryotic cell; (2) maintaining said cell under conditions appropriate for integrating said vector into the genome of said cell by non-homologous recombination whereby said transcriptional regulatory sequence and unpaired splice donor sequence are operably linked to said endogenous gene, said splice donor sequence being spliced to a splice acceptor sequence in said gene; (3) maintaining said cell under conditions appropriate for expressing said endogenous gene in said cell by means of said transcriptional regulatory sequence; and (4) maintaining said cell so as to produce amounts of the protein encoded by said endogenous gene.