Patent ID: 8779238
Filing Date: 2014-07-15
Classification: C12N

Abstract:
1. A method of transforming a Camelina plant comprising within a first dipping cycle, i) contacting the ii) removing the plant from the first transformation dipping solution, thereby conducting a first contacting step to produce a first dipped plant; iii) incubating the first dipped plant; iv) in the same first dipping cycle, contacting the first dipped plant with a second transformation dipping solution comprising a sugar, a nonionic surfactant, and an v) removing the twice dipped plant from the second transformation dipping solution; and vi) incubating the twice dipped plant; wherein the sugar is a disaccharide, the nonionic surfactant is trisiloxane, and both flowers and buds are present during the dipping steps; wherein said method does not involve vacuum infiltration.