Patent ID: 7776267
Filing Date: 2010-08-17
Classification: B01L,G01N,Y10T

Abstract:
1. A centrifugal force-based microfluidic device for protein detection, comprising: a body of revolution having a rotation center and a circumference; a microfluidic structure disposed in the body of revolution comprising a plurality of chambers, channels connecting the chambers to each other and forming a path of fluid flow between the chambers, and valves disposed in the channels to control the fluid flow between the chambers, the microfluidic structure transmitting fluid using centrifugal force due to rotation of the body of revolution, wherein one of the plurality of chambers receives a fluid biological sample beads disposed in the microfluidic structure, the beads having a first probe to capture a target molecule from a fluid biological sample, the first probe selectively binds to the target molecule; and a fluid containing a second probe disposed in the microfluidic structure and selectively binding to the target molecule, and which comprises a substance emitting an optical signal, wherein the microfluidic structure mixes the beads, the biological sample, and the second probe to bring them into contact with each other to produce a bead-target molecule-second probe complex, if the fluid biological sample contains the target molecule; and separates the bead-target molecule-second probe complex.