Patent ID: 6737278
Filing Date: 2004-05-18
Classification: G01N,Y10S

Abstract:
A method for determining an analyte in a sample comprising the steps of:i. applying the sample to an application zone for sample (ASZ) on a flow matrix in which transport of constituents present in the sample may take place in a transport flow, the flow matrix further comprising: a) an application zone (AR*Z) for an analytically detectable binding reactant (R*), b) a detection zone (DZ) which is located downstream of the ASZ and comprises another binding reactant (Capturer) firmly anchored to the matrix, so that a signal complex containing the Capturer and the analyte and/or R* is formed, and ii. detecting the signal complex in the detection zone DZ, the measured signal being used for determining the analyte, wherein the flow matrix comprises at least one separation zone (SZ) between the ASZ and the DZ, the SZ comprising an immobilized chemical structure which binds to at least one component present in the sample, without using the SZ as a mechanical obstacle, so that said component(s) substantially does (do) not disturb the detection of the analyte in the DZ.