Patent ID: 6117632
Filing Date: 2000-09-12
Classification: B82Y,C07K,G01N

Abstract:
A method of identifying a peptide which permits or facilitates the transport of an active agent through a human or animal tissue, comprising the steps of:(a) contacting a predetermined amount of phage from a random phage library or a preselected phage library to a first side of a tissue sample or polarized tissue cell culture;(b) at a predetermined time harvesting phage which is transported to a second side of the tissue sample or culture to select transported phage, which second side is opposite the first side;(c) amplifying the transported phage in a host;(d) repeating in order step (a) using the transported phage obtained in step (b) and amplified in step (c) and steps (b) and (c) a predetermined number of times to obtain a selected phage library containing phage which can be transported from the first side of the tissue sample or culture to the second side; and(e) determining the identity of at least one peptide coded by phage in the selected phage library to identify a peptide which permits or facilitates the transport of an active agent through a human or animal tissue.