Patent ID: 8722361
Filing Date: 2014-05-13
Classification: A61K,A61P,C07K,C12N

Abstract:
1. A method for production of a heterologous soluble polypeptide by a ciliate, the method comprising: (a) transforming said ciliate with a nucleic acid encoding a fusion protein comprising from N-terminus to C-terminus: wherein expression of said fusion protein results in trafficking of said fusion protein to mucocysts within said ciliate and cleavage of said mucocyst-targeting polypeptide to release said heterologous soluble polypeptide within said mucocysts; (b) stimulating regulated secretion from said mucocysts of said ciliate, whereby an extracellular matrix is formed; and (c) separating said heterologous soluble polypeptide from said extracellular matrix and said ciliates.