Patent ID: 9134308
Filing Date: 2015-09-15
Classification: C07K,G01N

Abstract:
1. A method of immobilizing an antibody to a solid support at a surface density of 1.14-1.15 ng/cm 2 , the method comprising: i) contacting the solid support with a linear or branched poly(ethylene glycol) (PEG) molecule, wherein the solid support has a surface having a first functional group and, wherein the PEG molecule comprises at least a second and a third functional group and wherein the second functional group reacts with the first functional group to form a covalent bond; ii) contacting the PEG molecule with a crosslinker, wherein the crosslinker comprises glutaraldehyde, and wherein the crosslinker has a fourth and a fifth functional group, wherein the fourth functional group reacts with the third functional group to form a covalent bond; and iii) contacting the crosslinker with the antibody comprising a sixth functional group, wherein the fifth functional group reacts with the sixth functional group to form a covalent bond, thereby immobilizing the antibody to the solid support, thereby immobilizing the antibody to a solid support at a surface density of 1.14-1.15 ng/cm