Patent ID: 8557536
Filing Date: 2013-10-15
Classification: G01N

Abstract:
1. A method of detecting articular cartilage degeneration or damage which cannot be detected in a radiograph, by using a concentration of keratan sulfate in a test sample derived from blood as an index, the method comprising: extracting a keratan sulfate fraction from serum digested with a pronase, by using an anion exchange column, to obtain a test sample; degrading keratan sulfate in the test sample into keratan sulfate disaccharide; measuring the concentration of keratan sulfate in the test sample by measuring a total amount of keratan sulfate disaccharide by high performance liquid chromatography; and evaluating articular cartilage degeneration or damage which cannot be detected in a radiograph, based on a comparison between the concentration of keratan sulfate measured in the test sample and the concentration of keratan sulfate in blood of a normal subject.