Patent ID: 6143513
Filing Date: 2000-11-07
Classification: C12Q,G01N

Abstract:
A method for detecting a betalactam-containing compound in a sample, comprising:contacting the sample with a D,D-carboxypeptidase and a substrate having a carboxyterminal D-alanine-D-alanine to form a reaction mixture, wherein the D,D-carboxypeptidase is capable of cleaving the terminal D-alanine from the substrate to yield a cleaved substrate;optionally contacting the reaction mixture with a ligand specific to the cleaved substrate or a ligand specific to the substrate to form a modified reaction mixture, wherein the ligand specific to the cleaved substrate is capable of binding to the cleaved substrate to form a ligand/cleaved substrate complex, and wherein the ligand specific to the substrate is capable of binding to the substrate to form a ligand/substrate complex; andcapturing on a sensing surface (i) the cleaved substrate or the substrate of the reaction mixture, or (ii) the ligand/cleaved substrate complex or the ligand/substrate complex of the modified reaction mixture, or (iii) the ligand specific to the cleaved substrate or the ligand specific to the substrate of the modified reaction mixture, by a surface-bound binding partner thereto.