Patent ID: 8906623
Filing Date: 2014-12-09
Classification: C12Q

Abstract:
1. A method for determining whether or not an unmethylated cytosine conversion treatment that converts unmethylated cytosine into a base other than cytosine is properly carried out, comprising the steps of: (A) converting unmethylated cytosine of the biological DNA contained in a sample into a base other than cytosine, to give an unmethylated cytosine conversion sample; (B) carrying out nucleic acid amplification reactions of the following (i) and (ii): (C) determining the amount of the amplification product obtained in the nucleic acid amplification reaction (i) and the amount of the amplification product obtained in the nucleic acid amplification reaction (ii) of the step (B); (D) calculating the ratio of the amount of the amplification product obtained in the nucleic acid amplification reaction (ii) to the amount of the amplification product obtained in the nucleic acid amplification reaction (i); and (E) determining whether or not the step (A) is properly carried out, based on the calculation result obtained in the step (D), wherein the nucleotide sequence not containing cytosine and the nucleotide sequence containing cytosine and not containing a CpG site are in a same chromosome of the biological body, and wherein the nucleotide sequence not containing cytosine and the nucleotide sequence containing cytosine and not containing a CpG site are contained in a nucleotide sequence of 300 bp or less in the nucleotide sequence of the biological DNA, wherein the biological DNA contains tumor cell DNA.