Patent ID: 6465175
Filing Date: 2002-10-15
Classification: C07H,C12Q

Abstract:
A method for detecting an oligonucleotide of interest in a sample, comprising:(a) providing a first oligonucleotide probe comprising (i) a first nucleic acid sequence complementary to a nucleic acid sequence in the oligonucleotide of interest, and (ii) a label wherein the label is a fluorescent dye that comprises a dipyrrometheneboron difluoride compound that, when the probe is in single-stranded, nonhybridized form, provides detectable emission radiation that, when the probe hybridizes to a complementary nucleic acid strand, is substantially quenched; (b) combining the first oligonucleotide probe with the sample suspected of containing the oligonucleotide of interest under hybridizing conditions to form a probe-oligonucleotide of interest hybrid complex, while monitoring emitted radiation from the first oligonucleotide probe; and (c) subsequent to step (b), correlating any change in emitted radiation with the presence or quantity of the oligonucleotide of interest.