Patent ID: 6281000
Filing Date: 2001-08-28
Classification: A61K,A61P,C12N

Abstract:
A recBC sbcBC strain of E. coli for the cloning of DNA fragments into the genome of a recombinant adenoviral plasmid vector by intermolecular homologous recombination, said recBC sbcBC strain comprising:(i) a first DNA fragment comprising all of said genome of the adenovirus or said adenoviral genome lacking all or part of the E1 region, E2 region, E3 region, E4 region or a combination thereof, and(ii) a second DNA fragment comprising a DNA sequence exogenous to said adenoviral genome surrounded by flanking sequences A and B which are homologous to sequences of (i), not including sequences of the 5'ITR, 3'ITR and encapsidation regions of said adenoviral genome, such as to allow intermolecular homologous recombination; wherein the first and second DNA fragments are introduced into said recBC sbcBC strain by separate vectors.