Patent ID: 6025324
Filing Date: 2000-02-15
Classification: A61K,Y10S

Abstract:
A composition comprising one or more conjugates of the formula ##STR3## where P is a homogeneous purified biologically active human obese protein comprising SEQ ID NO: 6 or fragment thereof, characterized in that said P1) when administered ICV to 16-18 hour fasted mature obese ob/ob mice having a body weight of at least 30 grams at a dose of 20 .mu.g or less,a) reduces food intake during a 5 hour feeding test by 50% compared to vehicle injected control mice (ED50 for reducing food intake); andb) reduces body weight gain during the 24 hours following the ICV injection by at least 50% compared to vehicle injected control mice (ED50 for reducing body weight gain);2) when administered IP to non-fasted mature ob/ob mice having a body weight of at least 30 grams twice a day at the beginning of daylight and again at the 3 hour point of the dark phase, for one week, in a total daily dose of 10 .mu.g or less.a) reduces 5 and 24 hour food intake by at least 20% compared to vehicle injected control mice (ED20 for reducing food intake); andb) reduces body weight gain during the 24 hours following the first IP injection by at least 20% compared to vehicle injected control mice (ED20 for reducing body weight gain).and n and n' are integers having a sum of from 300 to 1500; with the average molecular weight of the polyethylene or polypropylene glycol units in said conjugates within said composition being from 15 kDa to 60 kDa; and R and R' are lower alkyl.