Patent ID: 6060245
Filing Date: 2000-05-09
Classification: C12Q

Abstract:
A method for preferentially amplifying one or more desired nucleic acids comprising:preparing tester nucleic acids and driver nucleic acids from selected populations of nucleic acids;producing adaptor:tester nucleic acid complexes with said tester nucleic acids, wherein each adaptor:tester nucleic acid complex comprises a tester nucleic acid and a first adaptor comprising a first adaptor sequence, wherein said first adaptor sequence comprises a priming site;producing adaptor:driver nucleic acid complexes with said driver nucleic acids, wherein said adaptor:driver nucleic acid complex comprises a driver nucleic acid and a second adaptor comprising a second adaptor sequence, and wherein said first and second adaptor sequences are different;combining said adaptor:tester nucleic acid complexes and said adaptor:driver nucleic acid complexes so as to produce double-stranded nucleic acids comprising double-stranded adaptor:tester nucleic acid complexes, double-stranded adaptor:driver nucleic acid complexes, and double-stranded adaptor:tester/adaptor:driver nucleic acid complexes, under conditions such that said first adaptor sequences of the adaptor:tester nucleic acid complexes do not hybridize with said second adaptor sequences of the adaptor:driver nucleic acid complexes;adding a single-stranded nuclease that substantially cleaves single stranded nucleic acids; andamplifying said double-stranded adaptor:tester nucleic acid complexes with a reagent that selectively amplifies nucleic acids ligated to the first adaptor sequence.