Patent ID: 9150775
Filing Date: 2015-10-06
Classification: C09K,C12Y,E02D

Abstract:
1. A method for water tightening of water bearing zones and stabilization of sand in underground constructions by introducing an aqueous salt solution comprising Ca 2+ ions, plant based urease and urea into said zones and constructions to precipitate at least one mineral, wherein the plant based urease is prepared in the absence of a pH buffer by a method comprising: (a) grounding the plant wherefrom the urease is based; (b) adding water to the ground plant; (c) soaking the ground plant in the water at occasional stirring between 2 and 20 h at room temperature to achieve a solution; (d) filtrating the achieved solution; and (e) lyophilizing the filtrate to achieve a lyophilized urease.