Patent ID: 7229964
Filing Date: 2007-06-12
Classification: C07K,Y10S

Abstract:
1. A pharmaceutical composition for the treatment of diabetes in a patient in need of such treatment, comprising a sodium phosphate buffer and a therapeutically effective amount of an insulin derivative having the following sequence: wherein Xaa at position A21 is any codable amino acid except Lys, Arg and Cys; Xaa at positions B1, B2, B3, B26, B27, B28, B29 and B30 are, independent of each other, any codable amino acid except Cys or deleted; and a lipophilic group W is attached to the amino group of the N-terminal amino acid of the B-chain in which the lipophilic group W has from 12 to 40 carbon atoms and optionally contains a group which can be negatively charged or a lipophilic group Z is attached to the carboxyl group of the C-terminal amino acid of the B-chain in which the lipophilic group Z has from 12 to 40 carbon atoms and optionally contains a group which can be negatively charged, provided that: (a) when B1-B2-B3 is Phe-Val-Asn and A21 is Asn and B26-B27-B28-B29-B30 is Tyr-Thr-Pro-Lys-Thr or Tyr-Thr-Pro-Lys-Ala, then the lipophilic group W or Z always contains a group which can be negatively charged; and (b) when B29 and B30 are deleted and the lipophilic group Z is present and B1, B2 and B3 are not deleted then B1-B2 is different from Phe-Val or B26-B27-B28 is different from Tyr-Thr-Pro or both B1-B2 and B26-B27-B28 are different from said sequences; and (c) when B29 and B30 are deleted and the lipophilic group Z is present and one of B1, B2 or B3 is deleted then the N-terminal amino acid of the B-chain is different from Val or the sequence B26-B27-B28 is different from Tyr-Thr-Pro or both the N-terminal amino acid of the B-chain and the sequence B26-B27-B28 are different from Val and Tyr-Thr-Pro respectively.