Patent ID: 8697369
Filing Date: 2014-04-15
Classification: A61K,A61P,C12N,C12Y,G01N

Abstract:
1. A method for screening a test substance for activation of a receptor associated with FGF21 activity which comprises: (a) introducing FGFR1c and FGFR3c genes and a betaKlotho gene into a cell that has endogenously expressed neither an FGF receptor nor betaKlotho, then allowing said test substance to act on a transformed cell expressing the FGFR1c or FGFR3c and the betaKlotho on the surface thereof and determining whether said test substance induced increased growth of said transformed cell or increased signalling activity in said transformed cell when compared with a transformed cell on which no test substance had acted; (b) introducing an FGFR4 gene and a betaKlotho gene into a cell that has endogenously expressed neither an FGF receptor nor betaKlotho, then allowing said test substance to act on a transformed cell expressing the FGFR4 and the betaKlotho on the surface thereof and determining whether said test substance induced neither increased growth of said transformed cell nor increased signalling activity in said transformed cell when compared with a transformed cell on which no test substance had acted and (c) introducing FGFR1c, FGFR2c or FGFR3c genes into a cell that has endogenously expressed neither an FGF receptor nor a betaKlotho then allowing said test substance to act on a transformed cell expressing the FGFR1c, FGFR2c or FGFR3c on the surface thereof and determining whether said test substance induced neither increased growth of said transformed cell nor increased signaling activity in said transformed cell as is the case with a transformed cell on which no test substance had acted; whereby a positive showing in step (a) and a negative showing in steps (b) and (c) indicate activation of said receptor.