Patent ID: 9175282
Filing Date: 2015-11-03
Classification: C12N,C12P

Abstract:
1. A process for producing an isomerized saccharide selected from the group consisting of D-mannose, D-talose, epimaltose, and epimaltooligosaccharide with a glucose polymerization degree of 3 or higher, comprising the steps of: (1) allowing a cellobiose 2-epimerase to act on a material saccharide selected from the group consisting of D-glucose, D-galactose, maltose, and maltooligosaccharide with a glucose polymerization degree of 3 or higher, at a temperature of 55° C. or more, for forming a corresponding isomerized saccharide selected from the group consisting of D-mannose, D-talose, epimaltose, and epimaltooligosaccharide with a glucose polymerization degree of 3 or higher; wherein said cellobiose 2-epimerase catalyzes the following isomerizations: (a) 2-Epimerization (b) Aldose-Ketose conversion (2) decolorizing, deionizing, and concentrating the isomerized saccharide formed in step (1), wherein said cellobiose 2-epimerase is selected from the group consisting of a cellobiose 2-epimerase having the amino acid sequence of SEQ ID NO:10 and a recombinant cellobiose 2-epimerase having a variant amino acid sequence of SEQ ID NO:10, where one or more but less than 10 amino acid residues in SEQ ID NO:10 are replaced, deleted, or added without altering the enzyme activities in (a) and (b).