Patent ID: 6309833
Filing Date: 2001-10-30
Classification: C12Q

Abstract:
A method for the strand displacement amplification, multiplex assaying, and detection of target nucleic acids of interest using a bioelectronic microchip comprising the steps of:a. introducing at least one of the target nucleic acids of interest onto a bioclectronic microchip having a plurality of electronically addressable capture sites;b. asymmetrically amplifying the target nucleic acids to form amplicons, wherein unequal populations of complementary first and second amplified strands of the target nucleic acids are formed, and wherein the target nucleic acids are amplified using at least one set of amplification primers that includes an unequal effective concentration ratio of extendable and cleavable upstroam primer to extendable and cleavable downstream primer, wherein the upstream and downstream primers are specific for the first and the second stand of the target nucleic acids, respectively;c. capturing the amplicons of the target nucleic acids onto specified capture sites of the microchip by a capture probe that has specificity for the amplicons; andd. detecting the presence of the captured amplicons.