Patent ID: 8993253
Filing Date: 2015-03-31
Classification: B01L,C07K,C12Q,G01N

Abstract:
1. A product for detecting a protease enzyme in a sample comprising: (i) a polypeptide comprising a chromogenic amino acid, wherein the chromogenic amino acid isosterically matches a natural amino acid and is flanked by at least one amino acid to the N terminus and at least three amino acids to the C terminus thereof, the amine group of the chromogenic amino acid has a pKa of less than 5 and the chromogenic amino acid is capable of reacting with a conjugated aldehyde, and wherein the polypeptide comprises a target sequence for a target protease which is capable of cleaving the peptide bond comprising the amino group of the chromogenic amino acid; and (ii) a solid support, on which the polypeptide is immobilized.