Patent ID: 8566039
Filing Date: 2013-10-22
Classification: G16B

Abstract:
1. A computer-implemented method for determining a quantitative measure for an RNA expression profile of a biological sample containing sequences of nucleotides, the method comprising: for each of a plurality of output sequences from the biological sample: calculating a count histogram for the reference sequence based on the alignment of the plurality of output sequences onto the reference sequence, the count histogram including a respective coverage count for each nucleotide position of the reference sequence, wherein calculating the count histogram includes: for each nucleotide position of the reference sequence: locating a first contiguous region in the count histogram where the coverage count for each nucleotide position in the first contiguous region is equal to or greater than a threshold; and calculating a first abundance score for the first contiguous region, wherein an abundance score includes an average of the coverage count for each nucleotide position in a contiguous region; wherein the method is performed by a computer.