Patent ID: 6093574
Filing Date: 2000-07-25
Classification: G01N,Y10T

Abstract:
A method of placing a consistent amount of fluid on a slide in an automated biological reaction apparatus, the automated biological reaction apparatus having at least one rinse station, the rinse station comprising a rinse station nozzle positioned for directing a stream of fluid onto the slide, the rinse station nozzle connected to tubing which is connected to at least one valve, the valve connected to a bottle containing fluid, the valve controlling the flow of fluid from the bottle to the nozzle, the method comprising the steps of:pressurizing the tubing with a predetermined amount of pressure;turning on and off the valve for supplying fluid to the nozzle at least one time thereby causing a wave effect in the tubing wherein the pressure in the tubing varies from the predetermined amount of pressure, the time between turning on and turning off the valve being a first predetermined time;delaying until the wave effect wave effect in the tubing is lessened so that the pressure in the tubing is substantially equal to the predetermined amount of pressure;turning on the valve to begin a consistency pulse and directing a stream of fluid onto the slide;waiting for a second predetermined time, the second predetermined time being greater than the first predetermined time; andturning off the valve to end the consistency pulse.