Patent ID: 9145553
Filing Date: 2015-09-29
Classification: B01L,C12N

Abstract:
1. A method for isolating nucleic acids from an environmental sample, comprising: (i) obtaining an environmental sample comprising cells; (ii) subjecting the sample to a lysis process comprising mixing the sample with a lysis solution in the presence of liquid nitrogen, thereby obtaining a lysed material; (iii) subjecting the lysed material to an extraction process by mixing the lysed material with an extraction solution, and subjecting the resulting mixture to centrifugation; (iv) collecting the supernatant after the centrifugation; (v) washing the pellet obtained after the centrifugation of step (iii) with a wash solution comprising sodium phosphate at a concentration of at least 300 mM, and collecting the supernatant from the wash; and (vi) combining the collected supernatants from step (iv) and step (v), and isolating nucleic acids from the collected supernatants.