Patent ID: 6780326
Filing Date: 2004-08-24
Classification: B01D,G01N

Abstract:
A method of purifying a component of a sample, comprising:providing a column chamber, the column chamber having an inlet end and an outlet end, the outlet end terminating proximate both a first flow path and a second flow path, the first flow path being obstructed with a porous retaining material permeable to a first fluid and impermeable to a matrix material, and the second flow path being blocked by a blocking material that removably blocks flow of both the first fluid and the matrix material; flowing the first fluid and the matrix material into the column chamber and along the first flow path to form a packed column of the matrix material within the column chamber, the matrix material being configured to selectively retain a component of the sample; flowing the sample through the packed column and along the first flow path to separate the component from the rest of the sample; blacking the first flow path and removing the blocking material from the second flow path without moving the column chamber; and after removing the blocking material, flowing a second fluid through the column chamber and along the second flow path to remove the matrix material from the column chamber.