Patent ID: 6337188
Filing Date: 2002-01-08
Classification: C12Q

Abstract:
A method of sequencing a template nucleic acid molecule on a combinatorial array comprising the steps:A) immobilizing a sequencing reagent to a solid surface, wherein said sequencing reagent comprises: i) a capture moiety which can form a stable complex with a capture region of a template nucleic acid molecule; ii) a spacer region; and iii) a sequence specific hybridizing region, wherein said sequence specific hybridizing region comprises 4-8 bases, which are bound to the spacer region and which can hybridize to a complementary region on the template nucleic acid molecule; B) hybridizing said capture region of the template nucleic acid molecule to the capture moiety of the sequencing reagent; C) hybridizing said sequence specific hybridizing region to said complementary region on the template nucleic acid molecule thereby forming a hybridized sequence specific region; D) extending said hybridized sequence specific region by a polymerase with a chain terminating nucleotide thereby forming an extended sequence; and E) determining the identity of said extended sequence.