Patent ID: 7192772
Filing Date: 2007-03-20
Classification: C12N,C12Y

Abstract:
1. A recombinant host cell strain that is the product of a process comprising the steps of: (a) providing a culture comprised of enteric bacterial host cells comprising a pyruvate formate-lyase promoter which is endogenous to said host cells and a DNA encoding a pyruvate formate lyase gene under transcriptional control of said promoter; (b) transforming host cells in said culture with a heterologous DNA molecule comprising (c) selecting for host cells produced in step (b) that express said selectable marker polypeptide at a first level; (d) screening host cells obtained in step (c) to obtain host cells that produce said desired polypeptide at an initial level; (e) optionally exposing host cells identified in step (d) to a mutagen under conditions such that mutations are created in said DNA; and then (f) testing host cells produced in step (d) or step (e) for host cells that produce said marker polypeptide at a level higher than said initial level, to obtain host cells having a mutation that causes increased expression of the upstream genetic element resulting in an increase in production by said host cells of all polypeptides encoded by said heterologous DNA molecule compared to said production of all pblypeptides encoded by said heterologous DNA molecule by said host cells in the absence of said mutation, wherein said increased expression is retained in the absence of conditions that select for cells having said increased expression.