Patent ID: 6218112
Filing Date: 2001-04-17
Classification: A61K,C12Q

Abstract:
A method of screening test peptides for the ability to optimally transfect cells with a nucleic acid molecule comprising:detecting a change in the surface plasmon resonance of a nucleic acid immobilized on a sensor chip and exposed to a solution of a test peptide, wherein said change in surface plasmon resonance occurs upon binding of the peptide to and dissociation of the peptide from the immobilized nucleic acid, thereby to permit calculation of the equilibrium constant K.sub.D (or apparent dissociation constant, k.sub.d); andselecting the peptide having an equilibrium rate constant, K.sub.D, with a value of approximately 1.times.10.sup.-12 to 1.times.10.sup.-6 (or apparent dissociation constant, k.sub.d, with a value of approximately 1.times.10.sup.-6 to 1.times.10.sup.-1).