Patent ID: 8859218
Filing Date: 2014-10-14
Classification: C07K,C12Q,G01N

Abstract:
1. A method of detecting papillomavirus infection in a human subject and determining a risk of the human subject developing a cancerous lesion, comprising: receiving a sample obtained from the human subject, the sample comprising a population of cells susceptible to infection by a papillomavirus, applying the sample to a slide; contacting the sample with a first staining reagent comprising an anti-HPV monoclonal antibody generated against a purified recombinant papillomavirus protein, wherein the monoclonal antibody specifically binds to two or more native HPV proteins from different HPV types, wherein a positive staining is indicative of the presence of a papillomavirus protein in the sample thereby indicating papillomavirus infection in the human subject; and determining a nuclear to cytoplasm ratio in size for at least one cell of the population of cells; and determining a score for the sample based on the nuclear to cytoplasm ratio in size and the positive staining wherein the score indicates a risk of the human subject developing a cancerous lesion.