Patent ID: 6548021
Filing Date: 2003-04-15
Classification: B01J,B82Y,C07B,C07H,C12Q,C40B

Abstract:
A synthetic array comprising:a solid support, and a plurality of double-stranded nucleic acid molecules, wherein each nucleic acid molecule comprises a first nucleic acid strand linked to the solid support and a second nucleic acid strand which is substantially complementary to the first nucleic acid strand and hybridized to the first nucleic acid strand, wherein the 5â€² end of the first nucleic acid strand and the 3â€² end of the second nucleic acid strand are covalently unbound to each other, wherein each nucleic acid molecule comprises a protein recognition site within a double stranded nucleic acid sequence, wherein a protein recognition site within a first nucleic acid molecule is different from a protein recognition site within a second nucleic acid molecule, and a protein is bound to at least one of the nucleic acid molecules, wherein each protein recognition site within a nucleic acid molecule is selected from the group that includes naturally-occurring protein recognition sites, synthetic variants of naturally-occurring protein recognition sites and randomized nucleic acid sequences, and wherein at least one protein recognition site within a nucleic acid molecule comprises first and second half-sites, wherein the first half-site is recognized by a different protein than is the second half-site.