Patent ID: 9045712
Filing Date: 2015-06-02
Classification: C10G,C10L,C11B,C12N,C12P,C12Y,Y02E,Y02P

Abstract:
1. A method for degumming a crude oil comprising: i-a) mixing an aqueous acid with the crude oil to obtain an acidic mixture having pH of less than about 4; i-b) mixing a base with the acidic mixture to obtain a reacted mixture having pH of about 6-9, wherein the mixing in steps i-a) and/or i-b) creates an emulsion that comprises at least about 60% of an aqueous phase by volume in droplet size between about 15 μm to about 45 μm in size; and i-c) mixing a phospholipase enzyme with the emulsion in step (i-b), wherein the phospholipase enzyme comprises SEQ ID NO:8.