Patent ID: 8852868
Filing Date: 2014-10-07
Classification: C12Q

Abstract:
1. A real-time nucleic acid amplification reaction method for performing a nucleic acid amplification reaction in a droplet present in a container, wherein the droplet is composed of a nucleic acid amplification reaction liquid including a nucleic acid to be amplified and magnetic particles; the container holds a droplet encapsulating medium, and has a transport surface having a temperature gradient; the droplet encapsulating medium is insoluble or poorly soluble in the nucleic acid amplification reaction liquid; and a fluorochrome is initially added in the droplet encapsulating medium at the start of the nucleic acid amplification reaction, the method comprising transporting the droplet together with the magnetic particles by generating a magnetic field by means for applying a magnetic field to start and maintain a nucleic acid amplification reaction so that the droplet is placed on the transport surface at a temperature point at which the nucleic acid synthesis reaction is started and maintained, thereby controlling a temperature of the nucleic acid amplification reaction liquid.