Patent ID: 6271346
Filing Date: 2001-08-07
Classification: A61K,C07K,C12Q,G01N,Y02A,Y10S

Abstract:
A recombinant polypeptide which is nonglycosylated or is glycosylated by a CHO cell and has the ability to bind to TNF, wherein said polypeptide is encoded by DNA selected from the group consisting of:A) DNA comprising the sequence:R.sup.2 GAT AGT GTG TGT CCC CAA GGA AAA TAT ATC CAC CCT CAA AAT AAT TCG ATT TGC TGT ACC AAG TGC CAC AAA GGA ACC TAC TTG TAC AAT GAC TGT CCA GGC CCG GGG CAG GAT ACG GAC TGC AGG GAG TGT GAG AGC GGC TCC TTC ACC GCT TCA GAA AAC CAC CTC AGA CAC TGC CTC AGC TGC TCC AAA TGC CGA AAG GAA ATG GGT CAG GTG GAG ATC TCT TCT TGC ACA GTG GAC CGG GAC ACC GTG TGT GGC TGC AGG AAG AAC CAG TAC CGG CAT TAT TGG AGT GAA AAC CTT TTC CAG TGC TTC AAT TGC AGC CTC TGC CTC AAT GGG ACC GTG CAC CTC TCC TGC CAG GAG AAA CAG AAC ACC GTG TGC ACC TGC CAT GCA GGT TTC TTT CTA AGA GAA AAC GAG TGT GTC TCC TGT AGT AAC TGT AAG AAA AGC CTG GAG TGC ACG AAG TTG TGC CTA CCC CAG ATT GAG AATor a C- and/or N-terminally shortened sequence thereof, wherein R.sup.2 is absent or is a DNA comprising a sequence coding for a polypeptide which can be cleaved in vivo; andB) DNA comprising the sequence:R.sup.2 GAT AGT GTG TGT CCC CAA GGA AAA TAT ATC CAC CCT CAA AAT AAT TCG ATT TGC TGT ACC AAG TGC CAC AAA GGA ACC TAC TTG TAC AAT GAC TGT CCA GGC CCG GGG CAG GAT ACG GAC TGC AGG GAG TGT GAG AGC GGC TCC TTC ACC GCT TCA GAA AAC CAC CTC AGA CAC TGC CTC AGC TGC TCC AAA TGC CGA AAG GAA ATG GGT CAG GTG GAG ATC TCT TCT TGC ACA GTG GAC CGG GAC ACC GTG TGT GGC TGC AGG AAG AAC CAG TAC CGG CAT TAT TGG AGT GAA AAC CTT TTC CAG TGC TTC AAT TGC AGC CTC TGC CTC AAT GGG ACC GTG CAC CTC TCC TGC CAG GAG AAA CAG AAC ACC GTG TGC ACC TGC CAT GCA GGT TTC TTT CTA AGA GAA AAC GAG TGT GTC TCC TGT AGT AAC TGT AAG AAA AGC CTG GAG TGC ACG AAG TTG TGC CTA CCC CAG ATT GAG AAT GTT AAG GGC ACT GAG GAC TCA GGC ACC ACA,or a C- and/or N-terminally shortened sequence thereof, wherein R.sup.2 is absent or represents DNA coding for a polypeptide which can be cleaved in vivo;C) a DNA sequence of A or B encoding at least one conservative amino acid substitution;D) a DNA sequence of A or B encoding at least one amino acid substitution at a glycosylation site;E) a DNA sequence of A or B encoding at least one amino acid substitution at a proteolytic cleavage site; andF) a DNA sequence of A or B encoding at least one amino acid substitution at a cysteine residue.