Patent ID: 9149803
Filing Date: 2015-10-06
Classification: B01J,B01L,C12Q,G01N,Y10T

Abstract:
1. A fluidics system for delivering selected reagents to a flow cell, the fluidics system comprising: a fluidics circuit including a fluidics node having a fluid outlet in fluid communication with the flow cell, the fluidics node in fluid communication with at least one waste port through a plurality of passages without intervening valves, each passage of the plurality of passages providing a distinct fluid path between the fluidics node and the at least one waste port, each fluid inlet of a plurality of fluid inlets in unique fluid communication with a passage of the plurality of passages, the each fluid inlet of the plurality of fluid inlets uniquely coupled to an associated reagent reservoir of a plurality of reagent reservoirs, the fluidics circuit free of valves; a plurality of reagent valves, wherein each reagent valve of the plurality of reagent valves is uniquely disposed between a fluid inlet from the plurality of fluid inlets and the associated reagent reservoir of the plurality of reagent reservoirs; a wash inlet for introducing a wash solution from a wash valve to the fluidics circuit; and a control unit programmed to (a) deliver a sequence of selected reagents through the plurality of fluid inlets to the flow cell, and (b) in between delivery of each selected reagent, to wash the fluidics circuit with the wash solution and to prime the fluidics circuit with one of the selected reagents and the wash solution, wherein a portion of fluid flowing from a first fluid inlet of the plurality of fluid inlets flows through the fluidics node past a second fluid inlet of the plurality of fluid inlets and to the at least one waste port without entering the fluid outlet.