Patent ID: 7919328
Filing Date: 2011-04-05
Classification: G01N

Abstract:
1. A method for obtaining an accurate fluorescence image of one or more test samples, said method comprising the steps of: illuminating at least a portion of a substantially uniform fluorescing material that is doped with a plurality of fluorescent ions or rare earth ions; capturing fluorescence emitted from the fluorescing material; creating a first fluorescence image based on the captured fluorescence emitted from the fluorescing material; illuminating the one or more test samples located on a non-fluorescing material, wherein the one or more test samples contain a fluorescent dye; capturing fluorescence emitted from the one or more test samples located on the non-fluorescing material; creating a second fluorescence image based on the captured fluorescence emitted from the one or more test samples on the non-fluorescing material; and comparing the first fluorescence image and the second fluorescence image to create a normalized fluorescence image of the one or more test samples which are located on the non-fluorescing material.