Patent ID: 8029980
Filing Date: 2011-10-04
Classification: A61K,A61P,C12Q,G01N

Abstract:
1. A method for identifying a compound that inhibits FoxM1 transcriptional activity in vitro, comprising: (A) providing a cell comprising (i) a FoxM1 gene that encodes a polypeptide having the amino acid sequence as identified by SEQ ID NO: 14 operably linked to a first transcription control unit responsive to an effector, (ii) a second gene encoding a first reporter protein operably linked to a second transcriptional control unit responsive to FoxM1; and (iii) a third gene encoding a second reporter protein operably linked to a third transcription control unit for constitutive transcription thereof, wherein said first and second reporter proteins engender first and second detectable signals that are distinguishable from each other, (B) exposing said cell to said effector and optionally to the compound and measuring said first and second signals in the absence and in the presence of said compound, wherein the cell is exposed to a level of said effector that maximizes the signal engendered by said first reporter protein, and (C) identifying a compound that inhibits FoxM1 transcriptional activity when the levels of the first signal are decreased in the presence of the compound relative to the levels of the first signal in the absence of the compound, and wherein the levels of the second signal are substantially the same in the presence and absence of the compound.