Patent ID: 8871456
Filing Date: 2014-10-28
Classification: C07K,C12N,G01N

Abstract:
1. A method for preparing a protein conjugate, the method comprising: contacting a fusion protein with a lipoic acid analog in the presence of a lipoic acid ligase polypeptide to produce a protein conjugate in which the lipoic acid analog is linked to the fusion protein, wherein: the lipoic acid analog is a substrate of the lipoic acid ligase polypeptide and comprises either a functional group handle or a directly detectable moiety, the fusion protein comprises the target protein and an acceptor polypeptide, the acceptor polypeptide consists of the amino acid sequence Xaa the lipoic acid ligase polypeptide comprises the amino acid sequence of a wild-type lipoic acid ligase, or is a functional mutant of a wild-type lipoic acid ligase, which has an amino acid sequence at least 85% identical to SEQ ID NO:11, has lipoic acid ligase activity, and comprises an amino acid residue substitution at the position corresponding to W37 in SEQ ID NO:11.