Patent ID: 8293511
Filing Date: 2012-10-23
Classification: A61P,C12N

Abstract:
1. An isolated non-ribosomal peptide synthetase (NRPS) that catalyses formation of the tripeptide hydroxyphenylglycyl-cysteinyl-valine or the tripeptide phenylglycyl-cysteinyl-valine from hydroxyphenylglycine, cysteine and valine or from phenylglycine, cysteine and valine, respectively, the NRPS comprising (i) a first module M1 specific for hydroxyphenylglycine or phenylglycine comprising SEQ ID NO: 2 or SEQ ID NO: 4 or, where the first module M1 is specific for hydroxyphenylglycine the first module M1 comprises a sequence additionally selected from the sixth module of a Calcium-Dependent Antibiotic (CDA) synthetase of a (ii) a second module M2 specific for cysteine comprises SEQ ID NO: 6 or SEQ ID NO: 8 or a second module of an δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine synthetase of a Nocardia lactamdurans or a second module of an δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine synthetase of a (iii) a third module M3 specific for valine having SEQ ID NO: 10 or a third module of an δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine synthetase of a Nocardia lactamdurans or a third module of an δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine synthetase of a