Patent ID: 6406920
Filing Date: 2002-06-18
Classification: G01N,Y10S

Abstract:
In the process for carrying out a specific binding assay comprising the steps of reacting (a) a sample under assay, possibly containing a substance being tested for, with (b) a specific binding partner for the substance being tested for, immobilised on a solid support, and (c) a specific binding partner for the substance being tested for which is conjugated to a detectable marker, thereby to form a sandwich complex by reaction between whatever quantities are present of the substance being tested for with reagents (b) and (c) and immobilising the marker to the support via the substance being tested for, the marker being detected or assayed as an index of the quantity of the substance being tested for present in the sample (a), the improvement which comprises using reagents (b) and (c) together for reaction with sample (a) and avoiding competitive interference between the binding reactions of the substance being tested for and reagents (b) and (c) by using, as reagents (b) and (c), monoclonal antibodies each of narrow and different, non-interfering specificity.