Patent ID: 6544731
Filing Date: 2003-04-08
Classification: A61P,B01J,C07K,C12N,C40B,Y10S

Abstract:
A method of producing a memeber of a specific binding pair, wherein said specific binding pair member is an antibody or antibody fragment, comprising a synthetic antibody VH domain and an antibody VL domain, and having an antigen binding site with binding specificity for a human self antigen of interest, the method comprising:(a) providing a library of filamentous bacteriophage particles displaying on their surface a specific binding pair member, which specific binding pair member is an antibody or antibody fragment comprising an antibody VL domain and a synthetic antibody VH domain encoded by a VH, a DH, and a JH gene segment wherein said library is created by (i) providing a library of nucleic acid sequences encoding a genetically diverse population of said specific binding pair member, wherein in genetically diverse population of VH domain encoding sequences is provided by artificial rearrangement of about 50 human germ-line VH gene segments with DH and JH gene segments; (ii) expressing said library of nucleic acid sequences in recombinant host cells whereby each said specific binding pair member is displayed at the surface of a bacteriophage particle, wherein genetic material of said bacteriophage particle encodes said displayed specific binding pair member or a polypeptide chain thereof; (b) selecting one or more specific binding pair members having a binding specificity for said human self anitgen of interest, by binding said one or more specific binding pair members with said human self antigen of interest, each thus selected specific binding pair member being displayed on said particle.