Patent ID: 6391561
Filing Date: 2002-05-21
Classification: C07K,C12Q

Abstract:
A method for increasing the number of Kv channels formed by a Kv&agr; subunit on the plasma membrane of a host cell, comprising the steps of:(a) providing a cRNA molecule comprising a nucleotide sequence encoding a KChAP protein or variant thereof, wherein said KChAP protein or variant thereof comprises an amino acid sequence that is at least 95% identical to SEQ ID NO:2 or SEQ ID NO: 4, and binds to said Kv&agr; subunit; (b) providing a cRNA molecule comprising a nucleotide sequence encoding said Kv&agr; subunit; wherein the nucleotide sequence encoding said KChAP protein or variant thereof and the nucleotide sequence encoding said Kv&agr; subunit may be on the same or different cRNA molecules; (c) introducing the cRNA molecule encoding said KChAP protein or variant thereof and the cRNA encoding said Kv&agr; subunit into the host cell; and (d) co-expressing the KChAP protein or the variant thereof and the Kv&agr; subunit, thereby increasing the number of Kv channels formed by the Kv&agr; subunit on the plasma membrane of the host cell.