Patent ID: 8883478
Filing Date: 2014-11-11
Classification: A61K,A61L,A61P,C12N,C12Y

Abstract:
1. A method of preparing one or more tissues or organs for xenotransplantation, comprising the steps of: i. incubating the tissue or organ with a polypeptide having alpha-galactosidase activity, thereby cleaving immunodominant alpha-1,3-linked terminal galactose residues from the tissue or organ, and ii. isolating the tissue or organ from the polypeptide and the enzymatically-cleaved immunodominant galactose residues, thereby rendering the tissue or organ suitable for xenotransplantation, the polypeptide comprising a sequence, the sequence comprising residues 1-329 of SEQ ID NO: 7, followed by nine contiguous amino acids DD(P/A)(V/I)N(V/I)HGT (SEQ ID NO:10), followed by residues 339-477 of SEQ ID NO: 7, followed by twenty-one contiguous amino acids DXXXW(Y/F)E(S/T)GXXXD(L/V)(L/T)I(K/R)XNXF (SEQ ID NO:11), followed by residues 499-595 of SEQ ID NO: 7, and wherein the polypeptide has alpha-galactosidase activity.