Patent ID: 7214492
Filing Date: 2007-05-08
Classification: C12Q

Abstract:
1. A method for monitoring an ecosystem of interest comprising: (a) generating an array comprising a plurality of oligonucleotides immobilized at known locations on a substrate, wherein each location on the substrate comprises an oligonucleotide having a sequence derived from a single, predetermined microbial operational taxonomic unit (OTU), and wherein the array comprises at least one oligonucleotide having the sequence as set forth in SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 32, SEQ ID NO: 40, SEQ ID NO: 98, or SEQ ID NO: 100, or SEQ ID NO: 106, the complement of the sequence as set forth in SEQ ID NO: 25, SEQ ID NO: 26, SEQ ID NO: 32, SEQ ID NO: 40, SEQ ID NO: 98, SEQ ID NO: 100 or SEQ ID NO: 106; (b) preparing a nucleic acid sample from the ecosystem of interest; (c) hybridizing the nucleic acid sample to the array; (d) measuring hybridization of the nucleic acid sample to the array; and (e) correlating hybridization of the nucleic acid sample to the array with at least one parameter that comprises at least part of the ecosystem of interest.