Patent ID: 8962252
Filing Date: 2015-02-24
Classification: B01L,C12Q,G01N,H04N

Abstract:
1. A computer-implemented method for analyzing thermal melt data from an image of a reaction within a microchannel of a microfluidic device, comprising: illuminating at least a portion of the microchannel, wherein the microchannel is in thermal communication with a heat source; generating, with a pixel array sensor, an image of fluorescence emitted by material within the illuminated portion of the microchannel, wherein each pixel of the image has a JPEG value; defining a region of interest (“ROI”) comprising a portion of the pixels of the pixel array and subdividing the ROI into two or more sub-ROIs, each sub-ROI corresponding to a uniformly heated portion of the microchannel; and calculating the intensity of fluorescence emitted by material within the microchannel by averaging the JPEG values of all the pixels in each sub-ROI corresponding to a uniformly heated portion of the microchannel.