Patent ID: 6451353
Filing Date: 2002-09-17
Classification: A61K,G01N

Abstract:
A method for identifying by chromatography a composition of Fagopyrum cymosum (Trev.) Meisn capable of treating cancer comprising the steps of:a) preparing the assay comprising the steps of: I. ultrasonically dissolving 0.5 g. of Fagopyrum cymosum (Trev.) Meisn composition with 30 ml. of distilled water for 30 minutes to form a solution; II. removing the solution to the separator funnel; extracting the solution from the water with chloroform (30 ml. each time for 3 times) in order to degrease; III. continuously extracting the water layer from said solution with acetic ether (30 ml. each time for 5 times) to form a fraction; IV. collecting and blending the acetic ether fraction to form a blend; V. drying the blend with anhydrous calcium chloride; VI. filtering the blend to form a filtrate; VII. drying the filtrate; VIII. mixing said filtrate with water in constant volume of 10 ml. to form a suspension; IX. injecting 1 ml. of the suspension into C18ods cartridge and eluting it with 5 ml. of water, 5 ml. of 50% methanol and 5 ml. of methanol respectively; X. making the eluent of 50% methanol the constant volume of 5 ml.; and XI. filtering the eluent with 0.45&mgr; filtration membrane; b). using (âˆ’) epicatechin as the standard; and c). performing HPLC assay under following conditions: I. Gradient mobile phase: Time (minutes)Water (PH = 3.00)Acetonitrile09010407426502080II. column temperature: room temperature; flow rate: 0.8 ml/min; III. injection volume: 10 &mgr;l; IV. wavelength: 282 nm; and V. calculating according to the following formula: (âˆ’) epicatechin=AsÃ—Cst/AstÃ—WsÃ—100% wherein As=peak area of sample, Cst=concentration of standard, Ast=peak area of standard, Ws=weight of sample.