Patent ID: 9127067
Filing Date: 2015-09-08
Classification: A61P,C07K

Abstract:
1. A method of treating respiratory diseases with aberrant CXCR2 signaling comprising administering to a subject an effective amount of an isolated polypeptide comprising a first immunoglobulin single variable domain and a second immunoglobulin single variable domain, wherein each domain binds to the chemokine receptor CXCR2, and wherein the first immunoglobulin single variable domain binds a linear peptide consisting of the sequence of amino acids set forth in SEQ ID NO: 7 and the second immunoglobulin single variable domain does not bind to said linear peptide, wherein said isolated polypeptide has one of the following structures: a. FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4--LINKER--FR5-CDR4-FR6-CDR5-FR7-CDR6-FR8; b. FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4--LINKER--FR5-CDR4-FR6-CDR5-FR7-CDR6-FR8--LINKER--HLE; or c. FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4--LINKER--HLE--LINKER--FR5-CDR4-FR6-CDR5-FR7-CDR6-FR8; wherein if the first immunoglobulin single variable domain comprises FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4 then the second immunoglobulin single variable domain comprises FR5-CDR4-FR6-CDR5-FR7-CDR6-FR8; and if the second immunoglobulin single variable domain comprises FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4 then the first immunoglobulin single variable domain comprises FR5-CDR4-FR6-CDR5-FR7-CDR6-FR8; and the HLE is a binding unit providing an increased in vivo half-life; and wherein said second immunoglobulin single variable domain comprises 3 CDRs having the amino acid sequences set forth in SEQ ID NOs: 141, 236 and 181 and said first immunoglobulin single variable domain comprises 3 CDRs having the amino acid sequence set forth in SEQ ID NO: 146, 237 and 186.