Patent ID: 8849576
Filing Date: 2014-09-30
Classification: G01N,G16B,G16H,Y02A

Abstract:
1. A method of diagnosing a health status of a first specimen of mammalian tissue having a first specimen type by locating a clade corresponding to the first specimen on a cladogram for the specimen type, comprising the steps of: providing a table of ancestral value ranges for proteins of the specimen type, wherein the ancestral value ranges are defined as the range of one of the group of gene expression values for each gene in an examined range of genes and intensity values of mass spectrometry (“MS”) for each protein in an examined wavelength range in a group of healthy specimens of the first specimen type; providing a cladogram for the specimen type having separate clades corresponding to at least healthy, transitional and unhealthy specimens of the specimen type; making a polarity assessment by comparing one of the group of gene expression values or mass spectrometry intensity values for the first specimen to the first specimen type ancestral value ranges to determine whether the first specimen values are ancestral values defined as first specimen values within the ancestral value ranges or derived values defined as first specimen values outside the ancestral value ranges; creating a gene expression array for each gene expression value examined in the examined range of genes, wherein each field in the gene expression array is set to a first range of numbers designating an ancestral value if the corresponding gene expression value is within the ancestral value range and is set to a second range of numbers designating a derived value if the corresponding gene expression value is outside the ancestral value range; creating an MS intensity value array for each MS intensity value examined in the examined wavelength range, wherein each field in the MS intensity value array is set to a first range of numbers designating an ancestral value if the corresponding MS intensity value is within the ancestral value range and is set to a second range of numbers designating a derived value if the corresponding MS intensity value is outside the ancestral value range at the same wavelength; using a computer to apply a parsimony phylogenetic algorithm to an array selected from one of the group of the gene expression array and the MS intensity value array to determine a corresponding clade for the first specimen based on only the second range of numbers; diagnosing the health of the first specimen by determining the closest corresponding clade for the first specimen from the group of clades corresponding to at least healthy, transitional and unhealthy specimens of the specimen type.