Patent ID: 6656734
Filing Date: 2003-12-02
Classification: C12N

Abstract:
A process for transferring a therapeutically active polynucleotide into a target cell in vitro, wherein said cell is brought into contact with a composition comprising a mixture of at least one therapeutically active polynucleotide and at least one aprotic polar compound selected from the group consisting of:(a) formula I: â€ƒwherein R1 and R2 are identical or different alkyl radicals of 1 to 8 carbon atoms which are optionally substituted, with the proviso that when one of R1 or R2 is a radical of 1 or 2 carbon atoms, the other is a radical of at least 3 carbon atoms; wherein the volume of the aprotic polar compound represents from 5 to 50% of the total volume of the composition, and wherein the polynucleotide prior to being part of the mixture, is free of any compound which facilitates its introduction into a target cell.