Patent ID: 9150636
Filing Date: 2015-10-06
Classification: A61K,A61P,C07K,C12N,G01N

Abstract:
1. A method for assaying a candidate antibody for efficacy in killing a cancerous or infected cell, which method comprises: (i) selecting a candidate antibody that specifically binds to an antigen that is expressed by a tumor or infected cell, and that also binds to an NK-92 cell modified to express an FyγRIII-A receptor on a surface of the NK-92 cell, wherein said NK-92 cell is available from American Type Culture Collection (ATCC) as Accession No. PTA-6670, (ii) incubating in vitro a cancerous or infected cell with the candidate antibody to form a mixture under a condition that the antibody can bind to the cancerous or infected cell; (iii) incubating in vitro the mixture with the modified NK-92 cell under conditions wherein the modified NK-92 cell is capable of killing said cancerous or infected cell; and (iv) determining the efficacy of said antibody in killing said cancerous or infected cell.