Patent ID: 6432656
Filing Date: 2002-08-13
Classification: A61K,C07C,C07D,G01N,Y10S

Abstract:
A method for identifying a calcilytic compound that optimally inhibits one or more calcium receptor activities in a particular cell type, the method comprising:contacting a particular type of calcium receptor-bearing cell with a calcilytic compound having the formula: â€ƒwherein R1 is selected from the group consisting of: aryl, longer-length alk, and cyclo-alk; R2 is selected from the group consisting of: lower alk, cyclo-alk, alkoxy, H, OH, &boxH;O, C(O)OH, C(O)O-lower alk, C(O)NH-lower alk, C(O)N(lower alk)2, SH, S-lower alk, NH2, NH-lower alk, and N(lower alk)2; R3 and R4 are each independently lower alk or, together with the carbon to which they are attached, are cyclopropyl; R5 is aryl; R6 if present is either hydrogen, lower alkyl or optionally substituted lower alkenyl, wherein R6 is not present if R2 is &boxH;O; Y1 is either a covalent bond, alkylene, or alkenylene; Y2 is alkylene; Y3 is alkylene; Z is selected from the group consisting of: a covalent bond, O, S, NH, N-lower alk, alkylene, alkenylene, and alkynylene, provided that if Z is either O, S, NH, or N-lower alk, then Y1 is not a covalent bond; further provided that Y1 and Z may together be a covalent bond, or a pharmaceutically acceptable salt or complex thereof; and determining the effect of said compound on a calcium receptor activity of said particular type of calcium receptor-bearing cell, wherein said calcilytic compound is identified by the inhibition of said calcium receptor activity.