Patent ID: 9090925
Filing Date: 2015-07-28
Classification: C07D,C12P

Abstract:
1. A process for the large-scale chemoenzymatic production of (6S)-5-methyl-5,6,7,8-tetrahydrofolic acid, also known as (6S)-5-methylTHFA, the process comprising the steps of: (1) reducing folic acid (FA) with zinc powder under basic conditions to yield dihydrofolic acid (DHFA); (2) stereoselectively reducing DHFA with dihydrofolate reductase (DHFR) in the presence of an NADP/NADPH recycling system, glucose and GluDH so as to yield (6S)-THFA; (3) converting (6S)-THFA to (6S)-5-methylTHFA in situ by conventional methods; and (4) isolating (6S)-5-methylTHFA as its calcium salt; wherein (6S)-5-methyl-THFA is produced without isolation of an intermediate.