Patent ID: 9062098
Filing Date: 2015-06-23
Classification: A61K,A61P,C07K,C12N,G01N

Abstract:
1. A method of testing a sample for the presence or absence of a human bocavirus, comprising testing the sample for the presence of a bocavirus molecule selected from the group consisting of: (a) a nucleic acid molecule comprising a nucleotide sequence that encodes a polypeptide with an amino acid sequence having at least 90% sequence identity to human bocavirus polypeptide NS1 as shown in SEQ ID NO: 3; (b) an isolated nucleic acid molecule comprising a nucleotide sequence that encodes a polypeptide with an amino acid sequence having at least 90% sequence identity to human bocavirus polypeptide NP-1 as shown in SEQ ID NO: 4; (c) an isolated nucleic acid molecule comprising a nucleotide sequence that encodes a polypeptide with an amino acid sequence having at least 90% sequence identity to a bocavirus VP1 capsid polypeptide from a human bocavirus ST1 isolate as shown in SEQ ID NO: 5; (d) an isolated nucleic acid molecule comprising a nucleotide sequence that encodes a polypeptide with an amino acid sequence having at least 90% sequence identity to human bocavirus VP2 capsid polypeptide from a ST1 bocavirus isolate as shown in SEQ ID NO: 6; (e) an isolated nucleic acid molecule comprising a nucleotide sequence that encodes a polypeptide with an amino acid sequence having at least 90% sequence identity to human bocavirus VP1 capsid polypeptide from a ST2 bocavirus isolate as shown in SEQ ID NO: 7; (f) an isolated nucleic acid molecule comprising a nucleotide sequence that encodes a polypeptide with an amino acid sequence having at least 90% sequence identity to human bocavirus VP2 capsid polypeptide from a ST2 bocovirus isolate as shown in SEQ ID NO: 8; and (g) a polypeptide encoded by any of (a)-(f) wherein said testing comprises (i) adding first and second oligonucleotide primers to the sample, wherein the first primer is an isolated nucleic acid molecule between 10 and 30 nucleotides in length that specifically hybridises to the nucleotide sequence set forth in SEQ ID NO: 1 and/or SEQ ID NO: 2, and the second primer is an isolated nucleic acid molecule between 10 and 30 nucleotides in length that specifically hybridises to the complement of the nucleotide sequence set forth in SEQ ID NO: 1 and/or SEQ ID NO: 2; (ii) placing the sample in conditions for nucleic acid amplification; and (iii) testing the sample for the presence or absence of an amplification product, wherein detection of an amplification product indicates that the sample is positive for human bocavirus.