Patent ID: 8691574
Filing Date: 2014-04-08
Classification: C12N

Abstract:
1. A method for producing human iPS cells from hematopoietic progenitor cells, the method comprising the steps of: a) providing a cell population of non-mobilized human peripheral blood cells comprising hematopoietic progenitor cells; b) culturing said population under expansion conditions in a defined culture to promote the expansion of said hematopoietic progenitor cells; c) introducing exogenous episomal genetic elements comprising vectors that comprise an OriP replication origin, a nucleotide sequence encoding EBNA-1 or a derivative thereof which has a deletion of residues corresponding to residues 65 to 89 of EBNA-1, and/or a deletion of residues corresponding to residues 90 to 328 of EBNA-1, and one or more expression cassettes encoding iPS reprogramming factors into said expanded hematopoietic progenitor cells, wherein the exogenous episomal genetic elements have one or more polycistronic cassettes; and d) culturing said expanded hematopoietic progenitor cells under defined culture conditions, thereby producing human iPS cells from said hematopoietic progenitor cells.