Patent ID: 8835162
Filing Date: 2014-09-16
Classification: C07H,C12N,C12P

Abstract:
1. A method for incorporating an unnatural amino acid into a target protein at one or more specified position(s), the method comprising: (1) providing to a translation system a first polynucleotide encoding a modified tRNA (2) providing to the translation system a second polynucleotide encoding a modified Aminoacyl tRNA Synthetasel (AARS (3) providing to the translation system the unnatural amino acid, wherein the unnatural amino acid is a phenylalanine analog selected from a meta-substituted phenylalanine, or para-substituted phenylalanine, wherein the substitution comprises an aldehyde group, alkenyl group, alkynl group, an azido group, a halogen group, a hydrazide group, a hydrazine group a hydroxyl group, a keto group, a methoxy group, or a methyl group; (4) providing to the translation system a template polynucleotide encoding the target protein, wherein the codon(s) on the template polynucleotide for the specified position(s) forms Watson-Crick base-pairing with the modified tRNA (5) allowing translation of the template polynucleotide, thereby incorporating the unnatural amino acid into the target protein at the specified position(s), wherein steps (1)-(4) are effectuated in any order.