Patent ID: 8883421
Filing Date: 2014-11-11
Classification: C12N,C12Q

Abstract:
1. A method for ligating a 3′ adapter and a 5′ adapter to a target polynucleotide so as to avoid adapter dimer formation, comprising: (a) performing a first ligation between a 3′ end adapter and a target polynucleotide to form a first ligation product and excess 3′ adaptor; (b) adding a blocking oligonucleotide that hybridizes to the first ligation product and the excess 3′ adapter; and (c) ligating the first ligation product to a 5′ adapter, wherein hybridization of the blocking oligonucleotide to excess 3′ adapter prevents the excess 3′ adapter from ligating to the 5′ adapter.