Patent ID: 7282334
Filing Date: 2007-10-16
Classification: C12N

Abstract:
1. A method for generating a polynucleotide sequence or population of sequences from parent single stranded polynucleotide sequences encoding one or more protein motifs, comprising the steps of: a) providing single stranded polynucleotide sequences constituting plus and minus strands of parent polynucleotide sequences; b) digesting the single stranded polynucleotide sequences with an exonuclease to generate single stranded fragments; c) placing the single stranded fragments generated in step b) in conditions such that annealing occurs between fragments generated from the plus strands and fragments generated from the minus strands; and d) amplifying the fragments that anneal to each other to generate at least one polynucleotide sequence encoding one or more protein motifs having altered characteristics as compared to the one or more protein motifs encoded by said parent polynucleotides.