Patent ID: 6277569
Filing Date: 2001-08-21
Classification: C12Q

Abstract:
A method for the detection of at least three preselected target chromosomes or target chromosome regions by in situ hybridization using multiple direct label DNA probes comprising:(a) providing at least three direct label DNA probe compositions wherein each direct label DNA probe composition comprises direct label DNA segments which are complementary to a single target chromosome or target chromosome region and which have fluorescent labels covalently linked to the DNA segments wherein each of the direct label DNA compositions are detectable by use of a different fluorescent color, each color being detectable in the presence of the other direct label probe compositions;(b) contacting said at least three direct label DNA probe compositions under in situ hybridizing conditions with a target specimen comprising a plurality of target chromosomes or target chromosome regions; and(c) detecting the target chromosomes or target chromosome regions by visualizing three or more different fluorescent colors.