Patent ID: 7794929
Filing Date: 2010-09-14
Classification: A61P,C12Q

Abstract:
1. A method of identifying at least one methylation silenced gene associated with cancer, comprising: a) contacting an array of nucleotide sequences representative of a genome with nucleic acid subtraction products, wherein the nucleic acid subtraction products are nucleic acid molecules corresponding to RNA expressed in cancer cells that have been treated with a demethylating agent and an inhibitor of histone deacetylase, under conditions suitable for selective hybridization of the nucleic acid subtraction products to complementary nucleotide sequences of the array; and b) detecting selective hybridization of the nucleic acid subtraction products to a subpopulation of nucleotide sequences of the array, wherein the subpopulation of nucleotide sequences are not re-expressed in the presence of the histone deacetylase inhibitor alone and only re-expressed in the presence of the combination of at least one demethylating agent and at least one histone deacetylase inhibitor, wherein the detected subpopulation of nucleic acid sequences represents silenced genes consisting of methylation silenced genes comprising 5′ CpG methylated islands of the cancer cells, thereby identifying at least one methylation silenced gene associated with cancer, wherein the at least one methylation silenced gene comprises SFRP2.