Patent ID: 6479242
Filing Date: 2002-11-12
Classification: C12Q

Abstract:
A method for determining a nucleotide in a polynucleotide, comprising the following steps:a. providing a polynucleotide comprising at least one target site, and a first region of nucleotides immediately adjacent to the target site; b. then combining the polynucleotide with: a minisequencing primer complementary to the first region of nucleotides; three dideoxynucletides selected from the group consisting of ddATP, ddCTP, ddTTP and ddGTP; a deoxynucleotide selected from the group consisting of dATP, dCTP, dTTP and dGTP; wherein the nucleotide of the deoxynucleotide is not the same as the nucleotide in the dideoxynulceotide; and c. then extending the mini-sequencing primer with dideoxynucletide or deoxynulceotide whose base is complementary to the base at the target site, to provide extension products; and d. then analyzing the extension products with mass spectrometry.