Patent ID: 9127269
Filing Date: 2015-09-08
Classification: C12N

Abstract:
1. An isolated thermostable ligase, wherein the ligase is a mutant of a wild-type thermostable ligase, said wild-type thermostable ligase having a histidine adjacent to a KVDG motif, wherein the mutant thermostable ligase has a histidine to arginine mutation in its amino sequence at the histidine adjacent to the KVDG motif in the wild-type thermostable ligase, and wherein the wild-type DNA ligase has an amino acid sequence selected from the group consisting of SEQ ID NO: 26, SEQ ID NO: 27, SEQ ID NO: 28, SEQ ID NO: 29, SEQ ID NO: 30, SEQ ID NO: 31, and SEQ ID NO: 32.