Patent ID: 7575862
Filing Date: 2009-08-18
Classification: B01L,C12Q,G01N

Abstract:
1. A method for detecting a microorganism in a specimen, said method consists of the steps of: (a) extracting genomic DNA from the specimen; (b) performing the first polymer chain reaction (PCR) with an outer pair of primers using the genomic DNA as a template to obtain the first PCR DNA product; (c) performing the second PCR with an inner pair of primers using the first PCR DNA product as a template to obtain the second PCR DNA product; (d) hybridizing the second PCR DNA product with a probe attached to magnetic beads to obtain hybridized DNA fragments in a reaction container, in which the probe consists of a nucleotide sequence specific for, and is capable of, hybridizing with the second PCR DNA product; (e) removing unbound material and non-specific PCR products while subjecting the reaction container to a magnetic force; and (f) detecting hybridized DNA fragments to determine whether microorganism DNA is present in the specimen, wherein each pair of primers include respective forward and reverse primers, and each primer consists of a respective nucleotide sequence that is complementary to the genomic DNA of the microorganism, and the inner pair of primers bind to the first PCR DNA product.