Patent ID: 7485442
Filing Date: 2009-02-03
Classification: C07B,C07C,C07D,C07F,C07H,C09B,C12Q,C40B

Abstract:
1. A method for monitoring the amplification of a target nucleic acid sequence, comprising: (a) providing a mixture comprising a sample containing the target sequence, one or more oligonucleotide primers complementary to regions of the target sequence, a polymerizing enzyme, nucleotide substrates, and an oligonucleotide conjugate having a formula: wherein MGB is a minor groove binder, Q is a quencher, W is a trivalent linking group, ODN is an oligonucleotide or modified oligonucleotide, K is a bond or a linking group and Fl is a fluorophore, and said ODN portion has a sequence complementary to a portion of said target sequence being amplified; (b) incubating said mixture under conditions favorable for amplification of said nucleic acid sequence; and (c) continuously monitoring said amplification by monitoring the fluorescence produced upon conjugate hybridization to amplified target.