Patent ID: 6030823
Filing Date: 2000-02-29
Classification: C12N,C12P

Abstract:
A D-aminoacylase having the following physicochemical properties:(a) Action: acting on N-acetyl-D-amino acid to produce D-amino acid;(b) Molecular weight: about 55,000 daltons determined by SDS-polyacrylamide gel electrophoresis and about 36,000 to about 37,000 daltons determined by gel filtration;(c) Substrate specificity: acting on N-acetyl-D-methionine, N-acetyl-D-valine, N-acetyl-D-tryptophan, N-acetyl-D-asparagine, N-acetyl-D-phenylalanine, N-acetyl-D-alanine and N-acetyl-D-leucine, but not on N-acetyl-L-methionine, N-acetyl-L-leucine, and N-acetyl-L-valine;(d) Thermostability: stable at 40.degree. C. and inactivated at 50.degree. C. or above when heated at pH 7.5 for 30 minutes;(e) Optimum temperature: optimally active at about 45.degree. C. when reacted at pH 7.5;(f) Optimum pH: optimally active at a pH of about 7.5 to about 8.5 when reacted at 30.degree. C. for 30 minutes; and(g) Effects of metal ions: requiring no metal ion, but inhibited by 1 mM of Cu.sup.2+, Hg.sup.2+, Ni.sup.2+, Fe.sup.2+, Co.sup.2+, and Zn.sup.2+.