Patent ID: 8835169
Filing Date: 2014-09-16
Classification: C12N

Abstract:
1. A method of preparing a stem cell-enriched cell population, comprising the steps of: providing a biological material containing epidermis and dermis; dissociating dermis from epidermis by trypsinization and mechanical separation, in any order; collecting the epidermal cells from the trypsinized dermis; centrifuging and resuspending the cells in growth media with 2% serum to contain a concentration of about 20 million collected cells per 15 ml of growth media, wherein the growth media is devoid of bovine pituitary extract; incubating the cell suspension in a coated container, wherein the coated container is devoid of a xenogeneic feeder layer and bovine pituitary extract; removing the cell suspension containing unattached cells and adding serum-free, fatty acid-free media to the container containing attached cells in a ratio of media volume to standard container volume that is a multiple of the standard container volume; incubating the attached cells in the presence of excess media; and removing and resuspending cells which have grown and detached from the attached cells in the container.