Patent ID: 6063581
Filing Date: 2000-05-16
Classification: C07H,C12Q,G01N,Y10S

Abstract:
An immunological method for indirectly assaying homocysteine in a sample, said method comprising the steps of:(a) contacting said sample with S-adenosyl homocysteine hydrolase enzyme and adenosine or an analogue of adenosine, wherein said S-adenosyl homocysteine hydrolase converts said homocysteine into a non-labelled analyte, wherein said non-labelled analyte is S-adenosyl homocysteine or an analogue thereof; and(b) determining the presence or amount of the non-labelled analyte without chromatographic separation by contacting said sample with an antibody which specifically binds with said non-labelled analyte and with a detectable hapten for said antibody other than said non-labelled analyte, and wherein determining the presence or amount of said non-labelled analyte is effected indirectly by determining the presence or amount of said detectable hapten either bound or not bound to said antibody, wherein the amount of the non-labelled analyte is directly proportional to the amount of homocysteine in said sample.