Patent ID: 6492494
Filing Date: 2002-12-10
Classification: A61K,A61L,A61P

Abstract:
A method of forming a fibrin sealant from plasma comprising:(a) contacting the plasma, which contains alpha-2-antiplasmin, with an extraction implement that has both an alpha-2-antiplasmin-binding ligand and a first binding partner that is a member of a binding pair that comprises the first binding partner and a second binding partner, which partners bind to each other with an association constant of at least 105 Mâˆ’1; (b) isolating, from the plasma, the alpha-2-antiplasmin bound to the extraction implement; (c) contacting the plasma, which contains fibrinogen, with a fibrinogen-converting enzyme to form fibrin polymer and serum; (d) isolating from the plasma and acid solubilizing the fibrin polymer to form a first composition comprising acid-solubilized fibrin monomer; and (e) creating a fibrin sealant kit comprising, separately packaged, the first composition in an amount sufficient to form a clot, and a second composition comprising alpha-2-antiplasmin eluted from the isolated extraction implement in an amount sufficient to stabilize at least a clot-forming effective amount of the second composition, wherein the alpha-2-antiplasmin is isolated from the plasma prior to step (d).