Patent ID: 9201067
Filing Date: 2015-12-01
Classification: C12Q,G01N

Abstract:
1. A substrate for detecting the presence of a mutation in a gene, said substrate comprising a molecular layer of a plurality of regularly spaced size-controlled, cone shaped macromolecules, wherein each of said macromolecule comprises a polymer comprising a linear region and a plurality of branched regions in which a plurality of termini on the branched region are covalently bound to the substrate, and the terminus of the linear region is fixed with a target-specific oligonucleotide via a functional group, wherein said polymer is of the formula: wherein each of m, a, b, and c is independently 0 or 1; x is 1 when c is 0 or when c is 1, x is 3; y is 1 when b is 0 or when b is 1, y is 3; z is 1 when a is 0 or when a is 1, z is 3; n is 3; each of R Z is said target-specific oligonucleotide; and Y is carboxylic acid terminal group that is bound to the substrate; provided the product of n, x, y, and z is at least 3, and wherein said macromolecules are spaced at regular intervals between about 0.1 nm and about 100 nm between the linear functionalized groups.