Patent ID: 8697645
Filing Date: 2014-04-15
Classification: A61K,A61P,C07K

Abstract:
1. A method of modifying a hemoglobin, the method comprising introducing at least two chemical modifications to the hemoglobin, wherein each chemical modification by itself results in a hemoglobin with lower oxygen affinity than the same hemoglobin that is unmodified, wherein one of the chemical modifications comprises PEGylating the hemoglobin, wherein the hemoglobin comprises at least one thiol at a Cys-92(β) or a Cys-93(β), wherein the Cys-92(β) or the Cys-93(β) is protected before PEGylation, then deprotected after PEGylation, and wherein the Cys-92(β) or the Cys-93(β) is protected with dithiopyridine and deprotected with Tris(2-carboxyethyl)phosphine (TCEP).