Patent ID: 8652794
Filing Date: 2014-02-18
Classification: C12N,C12Q

Abstract:
1. An isolated recombinant luciferase having luciferase activity and comprising a variant of wild-type Photinus pyralis luciferase of SEQ ID NO: 37, wherein the amino acid sequence of said recombinant luciferase has no more than 50 amino acid differences as compared to the amino acid sequence of SEQ ID NO: 37, wherein the amino acid residue of the recombinant luciferase corresponding to position 295 of SEQ ID NO:37 is leucine, the amino acid residue of the recombinant luciferase corresponding to position 214 of SEQ ID NO:37 is an amino acid residue other than threonine, the amino acid residue of the recombinant luciferase corresponding to position 232 of SEQ ID NO:37 is an amino acid residue other than isoleucine, and the amino acid residue of the recombinant luciferase corresponding to position 354 of SEQ ID NO:37 is an amino acid residue other than glutamic acid, and wherein the recombinant luciferase has increased thermostability as compared to the wild-type Photinus pyralis luciferase of SEQ ID NO:37.