Patent ID: 8192931
Filing Date: 2012-06-05
Classification: C12Q

Abstract:
1. A method for determining a predisposition for developing prostate cancer or a presence of prostate cancer in a patient, said method comprising: (a) obtaining a crude urine sample from a patient, said crude urine sample comprising at least one prostate cell or nucleic acid extract thereof; (b) performing an RNA hybridization and/or amplification reaction on mRNA contained in said crude urine sample, using a first oligonucleotide or first primer pair specific to a prostate cancer-specific PCA3 mRNA molecule which is: (c) performing a second RNA hybridization and/or amplification reaction on mRNA contained in said crude urine sample, using a second oligonucleotide or second primer pair specific to a prostate-specific mRNA molecule; (d) detecting said PCA3 mRNA molecule and said prostate-specific mRNA molecule; and (e) determining, based on the presence or level of said prostate cancer-specific PCA3 mRNA molecule in said crude urine sample, that: