Patent ID: 7179611
Filing Date: 2007-02-20
Classification: C07K,C12P

Abstract:
1. A method for detecting the presence of C. difficile Toxin A in a patient comprising: coupling Toxin A antibodies to a hydrazide group containing resin; contacting a solution containing Toxin A with said resin so that said Toxin A is selectively retained on said coupled antibody resin; eluting purified Toxin A from said antibody resin with Tween 20; immunizing a host with said purified Toxin A collecting a sample from said host; isolating and purifying mono-specific polyclonal antibodies for combining said mono-specific polyclonal antibodies with latex particles in a manner whereby the polyclonal antibodies bind the latex particles; contacting a sample from said patient with the latex particles containing the polyclonal antibodies; and observing the presence or absence of agglutination, wherein the presence of agglutination indicates the presence of