Patent ID: 8431367
Filing Date: 2013-04-30
Classification: G01N

Abstract:
1. A method of detecting multiple analytes in a sample, the method comprising the steps of: obtaining a sample comprising at least one target nucleic acid and at least one target protein; introducing an aptamer that binds to the target protein in the sample; removing unbound aptamer; and conducting a single assay, wherein the assay detects both the target nucleic acid and the target protein, the assay comprising: performing a sequencing reaction on the target nucleic acid and the aptamer, wherein sequencing comprises: hybridizing a primer to each of the nucleic acid and the aptamer to form each of a nucleic acid/primer duplex and an aptamer/primer duplex; contacting each of the duplexes with a polymerase enzyme in the presence of at least one detectably labeled nucleotide under conditions that permit the polymerase to add nucleotides to the primers in a template-dependent manner; detecting a signal from the incorporated labeled nucleotide; and sequentially repeating the contacting and detecting steps at least once, wherein sequential detection of incorporated labeled nucleotide determines the sequence of each of the nucleic acid and the aptamer thereby detecting the nucleic acid and the aptamer in the sample.