Patent ID: 8685889
Filing Date: 2014-04-01
Classification: C12N,C12Q,C40B,G16B

Abstract:
1. A method for identifying one or more polymorphisms in nucleic acid samples, said method comprising the steps of: a) providing a plurality of nucleic acid samples of interest; b) performing a complexity reduction on each of the samples to provide a plurality of libraries of the nucleic acid samples, c) combining said libraries to form a combined library; d) ligating sequencing adapters carrying a overhang to the complexity reduced nucleic acid samples in the combined library; e) sequencing at least a portion of the combined library to obtain sequences; f) aligning each of the sequences obtained in step (e) to obtain an alignment; and g) determining one or more polymorphisms between the plurality of nucleic acid samples in the alignment of step (f).