Patent ID: 7943365
Filing Date: 2011-05-17
Classification: A23D,A23K,A23L,C12N,C12Y

Abstract:
1. A microbial host cell comprising an isolated polynucleotide comprising: (a) a nucleotide sequence encoding a polypeptide having Δ5 desaturase activity, wherein the polypeptide has at least 90% amino acid identity, based on the Clustal V method of alignment provided by the MegAlign™ program of the DNASTAR LASERGENE bioinformatics computing suite (default parameters: KTUPLE=1, GAP PENALTY=3, WINDOW=5 and DIAGONALS SAVED=5), when compared to an amino acid sequence as set forth in SEQ ID NO:13; (b) a nucleotide sequence encoding a polypeptide having Δ5 desaturase activity, wherein the nucleotide sequence has at least 90% sequence identity, based on the Basic Local Alignment Search Tool [“BLASTN”] method of alignment provided by the National Center for Biotechnology Information (default parameters: short queries=automatically adjust parameters for short input sequences, expect threshold=10, word size=11, match/mis-match scores=2, −3, gap costs=(existence: 5, extension: 2), and filter=low complexity regions), when compared to a nucleotide sequence as set forth in SEQ ID NO:12 or SEQ ID NO:25; (c) a nucleotide sequence encoding a polypeptide having Δ5 desaturase activity, wherein the nucleotide sequence hybridizes under stringent conditions to a nucleotide sequence as set forth in SEQ ID NO:12 or SEQ ID NO:25, wherein said stringent conditions include hybridization in 50% formamide, 1 M NaCl, 1% SDS at 37° C. and a wash in 0.1×SSC at 60° C. to 65° C.; or, (d) a complement of the nucleotide sequence of (a), (b) or (c), wherein the complement and the nucleotide sequence consist of the same number of nucleotides and are 100% complementary.