Patent ID: 9121849
Filing Date: 2015-09-01
Classification: C12Q,G01N,Y10T

Abstract:
1. A test strip for detection of at least one target nucleic acid in a sample comprising: at least one detection zone comprising at least one test zone; a sample application zone for applying the sample to the test strip; at least one mobile tagged nucleic acid reagent comprising a plurality of nucleic acid sequences complementary to a first portion of a sequence of the target nucleic acid and at least one tag portion comprising a first tag, wherein the mobile tagged nucleic acid reagent does not include a detectable label and wherein the mobile tagged nucleic acid reagent is loaded in a reagent zone; at least one second immobilized tag that binds to the first tag, wherein the immobilized tag is immobilized in a test zone on the test strip; and a running buffer that includes at least one detectable label, wherein the detectable label is a dye that binds to double stranded nucleic acids; and wherein the sample, the mobile tagged nucleic acid reagent, and the immobilized tag are all loaded on the test strip at locations such that the sample encounters the mobile tagged nucleic acid reagent and the immobilized tag while running an assay to detect the target nucleic acid in the sample.