Patent ID: 7531317
Filing Date: 2009-05-12
Classification: C12Q

Abstract:
1. A method of measuring protease activity comprising: (a) providing a protease substrate comprising a solubility tag and a fluorescence tag which is non-fluorescent until incorporated into a fluorescent complex, wherein the fluorescence tag comprises an amino acid motif Cys-Cys-Xaa-Xaa-Cys-Cys; (b) contacting the protease substrate of step (a) with a reagent capable of forming a fluorescent complex with the amino acid motif, wherein a fluorescent complex is formed; and (c) contacting the fluorescent complex of step (b) with a protease, under conditions wherein the protease is enzymatically active, to thereby yield a mixture; and then (d) measuring fluorescence polarization of the mixture of step (c), and determining the activity of the protease from the fluorescence polarization measured.