Patent ID: 6953663
Filing Date: 2005-10-11
Classification: B01J,B82Y,C07H,C12Q,C40B,G16B,Y10T

Abstract:
1. A method for detecting a polymorphism in a target nucleic acid sequence, comprising: receiving hybridization intensities for a first group of probes that are complementary to said target nucleic acid sequence except that the group of probes includes all possible monosubstitutions of positions in said sequence that are within n bases of a base in said sequence that is complementary to said polymorphism, wherein n is from 0 to 5; receiving hybridization intensities for a second and third group of probes complementary to marker-specific regions upstream and downstream of the polymorphism in the target nucleic acid sequence, wherein the third group of probes differs from the second set of probes at single bases corresponding to known mismatch positions; and identifying the polymorphism by analyzing the hybridization intensities.