Patent ID: 7052915
Filing Date: 2006-05-30
Classification: C07K,G01N,Y10S,Y10T

Abstract:
1. A method for selectively labeling or tagging one or more phosphate groups in a natural or synthetic peptide or protein which also contains one or more carboxylic acid groups or esters thereof which method comprises the steps of: a. reacting the natural or synthetic peptide or protein with a protective group that is an amine that reacts to protect the one or more phosphate groups therein by forming phosphoramide bonds and to protect any carboxylic acid groups and esters thereof therein by forming amide bonds; b. treating the protected peptide or protein under conditions which selectively substantially cleave the phosphoramide bonds, without substantially cleaving the amide bonds to regenerate one or more free phosphate groups in the peptide or protein; and c. reacting the one or more free phosphate groups in the peptide or protein, in which the carboxylic acids groups remain protected, with a label or tag comprising a functional group that reacts with a phosphate group to thereby generate a peptide or protein in which the phosphate groups are selectively labeled or tagged.