Patent ID: 6649409
Filing Date: 2003-11-18
Classification: A61K,C07K,C12N

Abstract:
A method for producing a nucleotide sequence construct comprising the following steps:a) obtaining a first nucleotide sequence of an HIV gene from a patient within the first 12 months of infection; b) designing a second nucleotide sequence utilising the most frequent codons from mammalian highly expressed proteins to encode the same amino acid sequence as the first nucleotide sequence of a) encodes c) redesigning the second nucleotide sequence of b) so that restriction enzyme sites surrounds the regions of the nucleotide sequence which encode functional regions of the amino acid sequence and so that selected restriction enzyme sites are removed thereby obtaining a third nucleotide sequence encoding the same amino acid sequence as the first and the second nucleotide sequence of a) and b) encode; d) redesigning the third nucleotide sequence of c) so that the terminal snuts contain convenient restriction enzyme sites for cloning into an expression vehicle; e) producing the snuts between restriction enzyme sites of c) and terminal snuts of d).