Patent ID: 6911313
Filing Date: 2005-06-28
Classification: G01N,Y10T

Abstract:
1. A method for discrimination and counting erythroblasts comprising the steps of: (i) staining leukocytes in a hematologic sample by adding a fluorescent leukocyte binding antibody to the hematologic sample to bind the leukocytes; (ii) raising the permeability only of cell membranes of erythroblasts in the hematologic sample to a nucleotide fluorescent dye which does not permeate a cell membrane when the permeability is not raised, the nucleotide fluorescent dye having a fluorescent spectrum that is distinguishable from that of a fluorescent labeling compound of the fluorescent labeled antibody in step (i); (iii) staining nuclei of the erythroblasts in the hematologic sample with the nucleotide fluorescent dye; (iv) analyzing the hematologic sample using flow cytometry to detect the nucleotide fluorescent signal of the stained erythroblasts and the fluorescent signal of the labeled antibody bound to the leukocytes; and (v) plotting the nucleotide fluorescent signal and the fluorescent labeled antibody signal in two coordinate axes to obtain a two-dimensional distribution chart discriminating between erythroblasts and leukocytes in the hematologic sample based on the difference in the two-dimensional distribution chart and counting the erythroblast.