Patent ID: 6322995
Filing Date: 2001-11-27
Classification: C12N,C12P

Abstract:
A recombinant gram positive bacterium selected from the group consisting of Corynebacter, Streptococcus, Staphylococcus, Lactococcus, and Bacillus which has been transformed by one or more copies of three exogenous DNA sequences, the first and second of said exogenous DNA sequences each comprising a DNA sequence coding for the riboflavin synthesizing enzymatic activities of Bacillus subtilis or a DNA sequence which encodes a polypeptide having riboflavin synthesizing enzymatic activity from Bacillus subtilis and one or more transcription elements and a third exogenous DNA sequence coding for the ribA gene product of Bacillus subtilis or a DNA sequence which encodes a polypeptide having GTP cyclohydrolase II or 3,4-dihydroxy-2-butanone 4-phosphate synthetase activity from Bacillus subtilis and one or more transcription elements wherein one or a plurality of copies of each of the first, second and third exogenous DNA sequences have been integrated at three different sites within its chromosome.