Patent ID: 7858321
Filing Date: 2010-12-28
Classification: C12Q,G01N,Y10S

Abstract:
1. A method for assaying for an analyte of interest comprising (a) introducing a sample comprising said analyte into a multi-well plate having a plurality of wells and at least two assay domains located within a single well of said plurality wells, said at least two assay domains including (i) a first domain comprising a first component that binds said analyte, and (ii) a second domain comprising a second component that competes with said analyte; (b) conducting in said single well a sandwich assay and a competitive assay, wherein the amount of said analyte is separately quantified in said sandwich assay and said competitive assay; and (c) comparing the amounts of said analyte identified in step (b) in said sandwich and said competitive assays to determine if a dynamic range of said sandwich assay has been exceeded.