Patent ID: 8084584
Filing Date: 2011-12-27
Classification: A61P,C07K

Abstract:
1. A method for generating a nucleic acid encoding a mutant antibody, the method comprising: (a) identifying an antibody that is susceptible to deamidation, wherein the antibody includes a first region that is a complementarity determining region (CDR); (b) identifying an asparagine residue susceptible to deamidation within the first region; (c) identifying a glycine residue located adjacent and C-terminal to the asparagine residue; and (d) producing a recombinant nucleic acid molecule that encodes a mutant antibody, wherein the mutant antibody is identical to the antibody of (a) except for an alteration selected from the group consisting of (i) a deletion of the glycine residue, and (ii) a substitution of the glycine residue with an amino acid residue other than valine, isoleucine or proline,