Patent ID: 6326185
Filing Date: 2001-12-04
Classification: C12C,C12N

Abstract:
An improved method for reducing colony forming units of acid-resistant bacteria in yeast comprising the steps of:(a) contacting the yeast with a tetrahydroiso-alpha hop acid in an amount sufficient to give a final concentration of at least about 40 parts per million (ppm);(b) holding the yeast mixture of step (a) for at least about five minutes;(c) after step (b), adjusting the pH of the mixture of step (b) to from about 2.0 to about 2.6; and(d) holding the yeast mixture of step (c) at a suitable temperature and for a period of time sufficient to reduce the colony forming units relative to a yeast control treated with tetrahydroiso-alpha acids at a concentration of 20 ppm.