Patent ID: 9063124
Filing Date: 2015-06-23
Classification: C07K,C12Q,G01N

Abstract:
1. A method for identifying a compound that modulates taste signaling in taste cells, the method comprising the steps of: (i) contacting the compound with a cell which expresses the taste transduction G protein-coupled receptor polypeptide encoded by a nucleic acid encoding a taste transduction G protein-coupled receptor having G protein-coupled receptor activity, the receptor comprising a polypeptide with at least 95% amino acid sequence identity to SEQ ID NO: 30, wherein said receptor detects bitter tastants, with the proviso that said isolated nucleic acid is not an isolated nucleic acid consisting of a genomic DNA; and (ii) determining the functional effect of the compound upon the polypeptide, thereby identifying a compound that modulates taste signaling in taste cells, wherein a modulation in the functional effect identifies a compound that modulates taste signaling.