Patent ID: 7163788
Filing Date: 2007-01-16
Classification: C12Q,G01N

Abstract:
1. A method of detecting the presence or absence of a molecular target in a sample, comprising: forming bead bound probes by attaching to a bead a first molecular probe capable of attaching to one part of the molecular target; forming support-bound probes by attaching to a support at a predefined area a second molecular probe capable of attaching to a second part of the molecular target, wherein said support is an optical disk having reflecting and non-reflecting binary features thereon and has an axis of rotation; introducing the sample to the bead-bound probes and the support-bound probes and allowing binding of the molecular target to said bead-bound probes and said support-bound probes so that the molecular target is sandwiched between the support and the beads, and the beads are attached on the support; aggregating beads that are not bound to the support around beads that are attached on the support; rotating the support about its axis of rotation; and determining the presence or absence of beads at the predefined area on the rotating support, wherein the detection of the beads on the rotating support indicates the presence of the molecular target in the sample.