Patent ID: 8883458
Filing Date: 2014-11-11
Classification: C07K,C12P

Abstract:
1. A process for preparing a desired L-amino acid by fermenting a recombinant microorganism of the Enterobacteriaceae family, which comprises: a) culturing the recombinant microorganism, which has been genetically modified to overexpress a polynucleotide encoding a polypeptide having acetate permease activity and an amino acid sequence that is at least 95% identical to SEQ ID NO: 2, in a fermentation medium under conditions under which the desired L-amino acid accumulates in the fermentation medium or in the recombinant microorganism, wherein the copy number of the polynucleotide encoding the polypeptide having acetate permease activity has been increased by at least 1 and/or a strong promoter is operably linked to the polynucleotide encoding the polypeptide having acetate permease activity; and b) concentrating the fermentation medium prior to recovery of the desired L-amino acid.