Patent ID: 6316600
Filing Date: 2001-11-13
Classification: C07K

Abstract:
A method for generating monoclonal antibodies comprising the steps of:(i) immunizing a chicken with an antigen composition;(ii) isolating B cells from said chicken;(iii) immortalizing said B cells;(iv) selecting an immortalized antibody producing B cell and preparing nucleic acids encoding the antigen binding exons of the light and heavy chain genes thereof;(v) cloning said heavy and light chain antigen binding regions, respectively, into vectors encoding(a) non-chicken constant and leader regions of an immunoglobulin heavy chain,(b) non-chicken constant and leader regions of an immunoglobulin light chain;(vi) transferring said vectors into a suitable host cell;(vii) culturing said host cell; and(viii) isolating antibodies produced by said cell.