Patent ID: 6111095
Filing Date: 2000-08-29
Classification: A61K,A61P,C07H,C12N

Abstract:
A method for producing a capped ribonucleic acid (RNA) or RNA analog oligonucleotide, comprising the steps of:a) reacting a RNA or RNA analog oligonucleotide with a phosphitylation agent to form a first intermediate;b) reacting the first intermediate with a phosphate or pyrophosphate, or a salt thereof, to form a diphosphite or cyclic triphosphite;c) oxidizing and hydrolyzing the diphosphite or the cyclic triphosphite to obtain a 5'-di- or 5'-triphosphorylated RNA or RNA analog oligonucleotide; andd) capping the di- or triphosphorylated RNA or RNA analog oligonucleotide by a reaction selected from the group consisting of (i) a reaction to enzymatically form the capped RNA or capped RNA analog molecule, and (ii) capping the di- or triphosphorylated RNA analog oligonucleotide by reacting with an activated m.sup.7 G monophosphate or 5' hydroxy analog.