Patent ID: 9192631
Filing Date: 2015-11-24
Classification: A61K,A61P,C12N

Abstract:
1. A method for obtaining human microglial precursor cells, comprising: (a) providing a cell population comprising neural precursor cells, wherein the cell population is obtainable from embryoid bodies differentiated from human pluripotent stem cells; (b) differentiating the cell population comprising neural precursor cells into microglial precursor cells by culturing in a medium comprising 5 to 500 μg/ml insulin, 100 ng/ml GM-CSF, 10 ng/ml IL-3 and 10 ng/ml M-CSF in the absence of FGF2, (c) expanding and enriching microglial precursor cells in a medium comprising 25 μg/ml insulin and 100 ng/ml GM-CSF; and (d) isolating microglial precursor cells positive for CD45.