Patent ID: 6943025
Filing Date: 2005-09-13
Classification: A01K,C12N

Abstract:
1. A method of identifying a fish with a gene mutation involved in carcinogenesis comprising the steps of: (a) exposing a fish to a mutagen; (b) mating said fish from step (a) with a wild-type fish to produce an F1 generation; (c) exposing haploid eggs derived from a female fish of said F1 generation to inactivated fish sperm to create haploid embryos; and (d) screening said haploid embryos for cell proliferation defects wherein an embryo with cell proliferation defects is determined to harbor a gene mutation involved in cell proliferation; (e) mating an F1 generation female harboring the gene mutation involved in cell proliferation as determined in step (d) with a wild-type fish to produce an F2 generation; (f) exposing a wild-type fish and a member of the F2 generation to a carcinogen; and (g) comparing the tumor formation in the wild-type and the member of the F2 generation fish wherein an accelerated tumor formation in the F2 generation fish identifies the fish with the gene mutation that is involved in carcinogenesis.