Patent ID: 8716465
Filing Date: 2014-05-06
Classification: A61P,C07K,C12N

Abstract:
1. A kit for making induced pluripotent stem (iPS) cells from a fibroblast cell comprising the following components: (a) a synthetic mRNA encoding OCT4, wherein each cytosine of the synthetic mRNA encoding OCT4 is replaced with 5-methylcytosine and each uracil is replaced with pseudouracil; (b) a synthetic mRNA encoding SOX2, wherein each cytosine of the synthetic mRNA encoding SOX2 is replaced with 5-methylcytosine and each uracil is replaced with pseudouracil; (c) a synthetic mRNA encoding KLF4, wherein each cytosine of the synthetic mRNA encoding KLF4 is replaced with 5-methylcytosine and each uracil is replaced with pseudouracil; and (d) a synthetic mRNA encoding c-MYC, wherein each cytosine of the synthetic mRNA encoding c-MYC is replaced with 5-methylcytosine and each uracil is replaced with pseudouracil.