Patent ID: 6156956
Filing Date: 2000-12-05
Classification: C12N

Abstract:
An expression cassette that generates, when contained in a plant host cell, complementary RNA which is sufficiently complementary to an RNA transcribed from an endogenous ACC synthase gene to prevent synthesis of said endogenous ACC synthase, wherein said expression cassette comprises the reverse transcript of said complementary RNA operably linked to control sequences which effect its transcription into said complementary RNA wherein said endogenous ACC synthase gene comprises a nucleotide sequence that is capable of amplification by a combination of primers selected from the group consisting of:a 5'.fwdarw.3' primer which encodes MGLAENQ (SEQ ID NO:12) and a 3'.fwdarw.5' primer which, read in the 5'.fwdarw.3' direction, encodes FQDYHGL (SEQ ID NO:2);a 5'.fwdarw.3' primer which encodes FQDYHG (positions 1-6 of SEQ ID NO:3) and a 3'.fwdarw.5' primer which, read in the 5'.fwdarw.3' direction, encodes FMEK(V/T)R (SEQ ID NO:13);a 5'.fwdarw.3' primer which encodes KA(L/V)EEAY (SEQ ID NO:14) and a 3'.fwdarw.5' primer which, read in the 5'.fwdarw.3' direction, encodes FPGFRVG (SEQ ID NO:15); anda 5'.fwdarw.3' primer which encodes KALEEAY (SEQ ID NO:16) and a 3'.fwdarw.5' primer which, read in the 5'.fwdarw.3' direction, encodes RVCFANMD (SEQ ID NO 9),wherein said control sequences comprise a tissue-specific promoter.