Patent ID: 7085765
Filing Date: 2006-08-01
Classification: G01N

Abstract:
1. A high throughput method of high content cell based screening assays, comprising: a) providing at least a first array of locations that contain multiple cells, wherein the cells comprise a first reporter set, wherein the first reporter set comprises at least a first fluorescent reporter molecule and a second fluorescent reporter molecule, wherein the fluorescent reporter molecules in the first reporter set b) conducting a primary screen that comprises imaging the at least first array of locations in high content mode to obtain fluorescent signals in the first channel from the fluorescent reporter molecules in the first reporter set, wherein the imaging occurs either before, after, and/or simultaneously with contacting of the at least first array of locations with one or more test compounds; c) detecting test compound induced changes in the fluorescent signals in the first channel from the fluorescent reporter molecules in the first reporter set, wherein a test compound induced change in the fluorescent signals in the first channel indicates an effect of the one or more test compounds on one or more cellular events reported on by the fluorescent reporter molecules in the first reporter set; and d) deconvolving the test compound induced changes, wherein the deconvolving comprises conducting one or more secondary screens, wherein at least one of the secondary screens comprises a method selected from the group consisting of: