Patent ID: 7885769
Filing Date: 2011-02-08
Classification: B01L,G01N

Abstract:
1. A screening method, in which binding quantity data each representing a quantity of binding of a single kind of a ligand to each of multiple kinds of analytes are acquired from measurement of a refractive index alteration quantity at an interface of an actual measurement region or a reference measurement region on a base body block, and in which an analyte having binding affinity to the ligand is identified from the multiple kinds of analytes and in accordance with the binding quantity data, the method comprising the steps of: i) performing an operation for bringing individual analyte solutions, each containing one of the multiple kinds of analytes, into contact with the actual measurement region, to which the ligand has been fixed, wherein multiple operations are performed corresponding to each of the analyte solutions, which each contain one of the multiple kinds of analytes, ii) acquiring values of actual measurement data from the multiple operations in i), wherein each value of actual measurement data represents a refractive index alteration quantity obtained from the corresponding operation, iii) performing an operation for bringing the analyte solution into contact with the reference measurement region, to which no ligand has been fixed, wherein multiple operations are performed corresponding to each of the analyte solutions, which each contain one of the multiple kinds of analytes, iv) acquiring values of reference measurement data from the multiple operations in iii), wherein each value of reference measurement data represents a refractive index alteration quantity and corresponds to the actual measurement data obtained from ii) using a same analyte, v) subtracting the values of the reference measurement data from the corresponding values of the actual measurement data, unprocessed binding quantity data being thereby acquired, vi) calculating a relationship between the values of the reference measurement data and the values of the unprocessed binding quantity data, which vary in accordance with the values of the reference measurement data, wherein the values of the reference measurement data and the values of the unprocessed binding quantity data are of analytes unbound to the ligand, vii) making calculation in accordance with the thus calculated relationship to find variation components, each of which is contained in one of the unprocessed binding quantity data and corresponds to one of the reference measurement data of all analytes unbound and bound to the ligand, viii) subtracting each of the values of the variation components, each of which is contained in one of the unprocessed binding quantity data, from the corresponding one of the values of the unprocessed binding quantity data, processed binding quantity data being thereby acquired, and ix) utilizing the thus acquired processed binding quantity data as the binding quantity data for use in identifying the analyte having binding affinity to the ligand.