Patent ID: 6251602
Filing Date: 2001-06-26
Classification: C07K,C12Q

Abstract:
A yeast cell comprising:a) a nucleotide sequence encoding a first heterologous fusion protein comprising a first peptide of a multiple peptide binding complex, or a segment thereof, joined to a transcriptional activation protein DNA binding domain;b) a nucleotide sequence encoding a second heterologous fusion protein comprising a second peptide of the complex, or a segment thereof, joined to a transcriptional activation protein transcriptional activation domain;wherein binding of the first peptide or segment thereof and the second peptide or segment thereof, which occurs in the presence of a third heterologous peptide of the multiple peptide binding complex, reconstitutes a transcriptional activation protein; andc) a reporter gene activated under positive transcriptional control of the reconstituted transcriptional activation protein, wherein expression of the reporter gene produces a selected phenotype.