Patent ID: 8628918
Filing Date: 2014-01-14
Classification: C12Q

Abstract:
1. A method of capturing ten or more different nucleic acids of interest, the method comprising: providing a sample comprising the ten or more different nucleic acids of interest; providing a pooled population of particles, the population comprising ten or more subsets of particles, the particles in each subset having associated therewith a different support capture probe, which support capture probe comprises only naturally occurring bases A, C, G, T, and/or U; providing ten or more subsets of at least two different target capture probes, which target capture probes comprise only naturally occurring bases A, C, G, T, and/or U, wherein a different subset of target capture probes is provided for each different nucleic acid of interest, wherein the at least two different target capture probes in each subset are capable of hybridizing to nonoverlapping polynucleotide sequences in the corresponding nucleic acid of interest, and wherein the target capture probes in each subset are capable of hybridizing to a different one of the support capture probes and thereby associating each subset of target capture probes with a different selected subset of the particles; contacting the sample, the pooled population of particles, and the subsets of target capture probes; and, for each of the nucleic acids of interest, hybridizing the nucleic acid to its corresponding subset of at least two different target capture probes and hybridizing the subset of target capture probes to its corresponding support capture probe, thereby capturing the nucleic acid on the subset of particles with which the target capture probes are associated, wherein the hybridizing the subset of at least two different target capture probes to the corresponding support capture probe is performed in the presence of the nucleic acid and at a hybridization temperature which is greater than a melting temperature T