Patent ID: 8703438
Filing Date: 2014-04-22
Classification: C12Q,G01N

Abstract:
1. A method of identifying a protein target that interacts with or is bound by a test agent, said method comprising: contacting a test sample comprising a plurality of proteins with said test agent to form a sample/test agent mixture; contacting said mixture with a protease where said protease is not said test agent; determining the abundance of a protein in said test sample/test agent mixture and in a negative control sample lacking said test agent; and identifying and/or selecting a protein or protein fragment that is protected from proteolysis, wherein said identifying comprises comparing the abundance of a protein in said test sample/test agent mixture to the abundance of a protein in said negative control sample, and the presence and/or abundance of a protein or protein fragment protected from proteolysis in said test sample is an indicator that said protein or protein fragment binds to or interacts with said test agent and is a target for said test agent.