Patent ID: 6277617
Filing Date: 2001-08-21
Classification: C11D,C12N

Abstract:
A modified subtilisin wherein one or more amino acid residues selected from the group consisting of amino acid residue 62, amino acid residue 217 amino acid residue 222, amino acid residue 156 and amino acid residue 166 are replaced by cysteine residues, wherein the cysteine residues are modified by replacing the thiol hydrogen with a substituent group providing a thiol side chain selected from the group consisting of:a) --SR.sup.1 R.sup.2, wherein R.sup.1 is an alkyl and R.sup.2 is a charged or polar moiety;b) --SR.sup.3, wherein R.sup.3 is a substituted or unsubstituted phenyl;c) --SR.sup.4, wherein R.sup.4 is a substituted or unsubstituted cyclohexyl; andd) --SR.sup.5, wherein R.sup.5 is C.sub.10 -C.sub.15 alkyl.