Patent ID: 8513403
Filing Date: 2013-08-20
Classification: A61K,C12N

Abstract:
1. A modified siRNA comprising a double-stranded region of about 15 to about 30 nucleotides in length, wherein from 15% to about 30% of the nucleotides in the double-stranded region comprise 2′-O-methyl (2′OMe) nucleotides, wherein the modified siRNA comprises 2′OMe nucleotides in both strands of the modified siRNA, wherein the modified siRNA comprises at least one 2′OMe-guanosine nucleotide and at least one 2′OMe-uridine nucleotide in the double-stranded region, wherein the modified siRNA does not comprise 2′OMe-cytosine nucleotides in the double-stranded region, wherein the modified siRNA is less immunostimulatory than a corresponding unmodified siRNA sequence, and wherein the modified siRNA is capable of silencing expression of a target sequence.