Patent ID: 8592185
Filing Date: 2013-11-26
Classification: C07K,C12P,C12Q

Abstract:
1. A method for enriching methyl-CpG sequences from a DNA sample comprising: a) providing a DNA sample; b) fragmenting the DNA by endonuclease digestion, restriction enzyme digestion or mechanical methods to form a plurality of double stranded fragments having a convenient size range; c) treating the plurality of double stranded fragments of b) under conditions suitable for conversion of all cytosine bases to uracil, which conditions are suitable for conservation of methylated cytosine bases, to form a plurality of cytosine-free double stranded DNA fragments; d) amplifying the cytosine-free fragments, or subset thereof, to form an amplified fragment population in which all uracil bases are replaced with thymidine and all methyl-cytosine bases are replaced with cytosine; e) methylating all cytosine bases in CpG dinucleotide sequences in the amplified fragment population of d); and f) collecting all methylated amplified fragments from e), thereby enriching for methyl-CpG sequences from the DNA sample.