Patent ID: 8530198
Filing Date: 2013-09-10
Classification: C12Q,G01N

Abstract:
1. A method of detecting multiple analytes in a sample, the method comprising the steps of: obtaining a sample comprising at least one target nucleic acid and at least one target protein; introducing an aptamer that binds to the target protein in the sample; and conducting a single assay, wherein the assay detects both the target nucleic acid and the target protein; wherein the assay comprises the steps of: performing PCR on the target nucleic acid; performing PCR on the aptamer; introducing a first detectably labeled probe that binds to the amplified target nucleic acid; introducing a second detectably labeled probe that binds to the amplified aptamer; detecting the first probe thereby detecting the nucleic acid in the sample; and detecting the second probe that is bound to the amplified aptamer, wherein detecting the second probe detects the target protein.