Patent ID: 9012374
Filing Date: 2015-04-21
Classification: B01J,C12Q,C40B

Abstract:
1. A method of manufacturing an array of products on a replica array comprising: delivering a continuous volume of a liquid reagent or reaction mix on the surface of a master substrate by laminar flow; providing partitioned reaction volumes of the liquid reagent or reaction mix on the master substrate, wherein the reaction volumes are partitioned by surface energetic barriers provided by adjacent hydrophobic regions and hydrophilic regions on the master substrate; generating products by one or more enzymatic processes in the partitioned reaction volumes of the liquid reagent or reaction mix; and immobilizing the products from the partitioned reaction volumes from the master substrate on capture moieties disposed on a replica array that is in contact with the partitioned reaction volumes, wherein partitioning of the reaction volumes prevents diffusion of products between them.