Patent ID: 8298822
Filing Date: 2012-10-30
Classification: A61K,C12N

Abstract:
1. A process for the generation of epithelial cells, comprising: aggregating stem cells from differentiated exocrine glandular tissue to an organoid body, and differentiating at least a part of the organoid body or of a tissue body grown therefrom to epithelial cells, wherein the differentiation comprises a cultivation of the organoid body or the tissue body on a boundary surface between liquid and gaseous media wherein at least one cell of the organoid body or the tissue body extends out of the liquid cultivation medium into the gaseous medium, wherein either i) a liquid surface of the cultivation medium is not shifted relative to the surface of the organoid body or tissue body during the cultivation on the boundary surface; or ii) a liquid surface of the cultivation medium is shifted relative to the surface of the organoid body or tissue body during the cultivation on the boundary surface and wherein the shifting of the liquid surface takes place at a rate relative to the surface of the organoid body or of the tissue body that is adapted to a conversion rate for differentiating cells of the organoid body or of the tissue body to epithelial cells.