Patent ID: 6531132
Filing Date: 2003-03-11
Classification: A61K,A61Q,C12N,Y10S

Abstract:
A process for extracting an active principle to guard against degradation of a dermo-epidermal junction of skin and to improve the surface condition of skin, comprising:solubilizing crushed Saccharomyces Cerevisiae in a basic aqueous medium to form a mixture, hydrolyzing proteins of the mixture in a basic medium by adding enzymes in the amount of 0.05% to 5% by volume, agitating the mixture, filtering the mixture to separate soluble and insoluble phases, obtaining an oligosaccharide active fraction, washing with water said oligosaccharide active fraction so as to purify said oligosaccharide active fraction from salts, proteins and peptides, concentrating and filtering said oligosaccharide active fraction to obtain an active principle, and sterile filtering said active principle.