Patent ID: 7700310
Filing Date: 2010-04-20
Classification: C12Q

Abstract:
1. A method for measuring ATP-dependent transferase enzymatic activity comprising: (a) providing a reagent composition comprising a detergent, a luminogenic molecule and a chemostable luciferase, wherein the detergent selectively stops ATP-dependent transferase activity without substantially affecting chemostable luciferase activity; (b) incubating a first reaction mixture comprising an ATP-dependent transferase, ATP, and a transferase substrate for a first predetermined time period under conditions effective to allow for a transferase reaction to occur; (c) contacting the first reaction mixture with the reagent composition to form a second reaction mixture and incubating the second reaction mixture for a second predetermined time period under conditions effective to simultaneously stop ATP-dependent transferase enzymatic activity and allow for a bioluminescent reaction to occur; and (d) determining transferase activity by measuring luminescence of the second reaction mixture.