Patent ID: 6740492
Filing Date: 2004-05-25
Classification: C12Q,G01N

Abstract:
A method of detecting electrophoretically separated biomolecules comprising the steps of;(a) obtaining a solid matrix having disposed thereon a plurality of electrophoretically separated biomolecules; (b) contacting the solid matrix with a collection of phage, wherein individual phage in the collection have a phage-expressed binding protein so that the collection of phage in aggregate comprises a collection of phage-expressed binding proteins, and wherein contact of the solid matrix and the collection of phage results in a non-bound population of phage and a bound population of phage; (c) removing the non-bound population of phage to leave the bound population of phage localized to positions on the solid matrix corresponding to positions of the electrophoretically separated biomolecules; (d) replicate plating by placing the bound population of phage in contact with a bacterial lawn having host bacteria for the phage under conditions that permit the bound phage to infect the host bacteria so as to produce a replicated population of phage; and (e) detecting the replicated population of phage, whereby electrophoretically separated biomolecules are detected.