Patent ID: 8280643
Filing Date: 2012-10-02
Classification: A61K,A61P,C07K,G01N

Abstract:
1. A method for determining the structure of a microbial surface components recognizing adhesive matrix molecule in complex with fibrinogen, comprising the steps of: providing a Clumping factor A ClfA complexed with a fibrinogen gamma-peptide; determining a ClfA binding region of the fibrinogen gamma-peptide; determining one or more critical amino acid residues in the ClfA binding region of a native fibrinogen gamma-peptide that is critical for a ClfA:fibrinogen gamma-peptide interaction; determining one or more amino acid residues of the ClfA that binds to the ClfA binding region of the native fibrinogen gamma-peptide; modeling the structure of the ClfA binding region; performing computational modeling of the ClfA sequence that binds to the ClfA binding region of native fibrinogen, thereby determining the structure of the ClfA:fibrinogen gamma-peptide interaction; and identifying one or more potential agents that inhibit the ClfA:fibrinogen gamma-peptide interaction without affecting binding of other proteins to the fibrinogen gamma-peptide.