Patent ID: 6780976
Filing Date: 2004-08-24
Classification: C12N,C12P

Abstract:
An isolated Kluyveromyces lactis reductase wherein the reductase is an enone reductase that:(a) reduces the carbon-carbon double bond of an &agr;,&bgr;-unsaturated ketone, using NADPH as an electron donor, to produce a corresponding saturated hydrocarbon; (b) has an optimal pH of 6.5-7.0; (c) has a molecular weight determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and by gel filtration of about 43,000 Da and about 42,000 Da, respectively; (d) has an optimal temperature within the range of 37-45Â° C.; and (e) has a substrate specificity of (1)-(4): (1) substantially no activity to reduce the keto group of a ketone; (2) exhibits a significantly higher activity with NADPH than with NADH as an electron donor; (3) does not substantially act on a substrate in which neither substituent at the &bgr; carbon relative to the ketone is hydrogen; and (4) does not substantially act on a substrate in which the carbon-carbon double bond is present in a cyclic structure.