Patent ID: 7824855
Filing Date: 2010-11-02
Classification: B01D,C12N

Abstract:
1. A method for selectively separating and purifying RNA from a mixture solution of nucleic acid containing DNA and RNA, the method using a cartridge for separation and purification of nucleic acid comprising a container having at least two openings, and the container receives a nucleic acid-adsorbing porous membrane which a solution can pass through, wherein the method comprising the steps of: (1-a) adsorbing nucleic acid to the nucleic acid-adsorbing porous membrane; (1-b) washing the nucleic acid-adsorbing porous membrane by a washing solution, while the nucleic acid is adsorbed to the nucleic acid-adsorbing porous membrane; (1-c) subjecting the nucleic acid-adsorbing porous membrane to a DNase treatment; (1-d) washing the nucleic acid-adsorbing porous membrane with the washing solution; and (1-e) desorbing the RNA from the nucleic acid-adsorbing porous membrane by a recovering solution, so as to discharge the recovering solution out of the cartridge, resulting in separated and purified RNA, wherein in the step (1-c), a total amount of a DNase solution is 130 μl or less per 1 cm2 of the nucleic acid-adsorbing porous membrane, and wherein the nucleic acid adsorbing porous membrane has (a) a front area and a back area asymmetrical with each other and (b) comprises an organic material obtained by saponification of a mixture of acetyl celluloses different from each other in acetyl value.