Patent ID: 9109258
Filing Date: 2015-08-18
Classification: C12Q

Abstract:
1. A method of diagnosing metastasizing endometrial carcinoma in a human patient, comprising: analyzing an endometrial carcinoma tissue sample from the patient for expression of EDI-3; wherein the expression level of EDI-3 is analyzed by: detecting and/or determining the expression of an RNA transcript of a nucleic acid encoding EDI-3 (SEQ ID NO: 7) or a fragment thereof by isolating total RNA from the tissue sample and performing quantitative PCR on the total RNA with primers suitable for amplification of a cDNA transcript of greater than 200 consecutive nucleotides of SEQ ID NO: 7 from the region between nucleotides 2872 to 3362 thereof; and diagnosing that the endometrial carcinoma of the patient is likely to metastasize when the level of expression EDI-3 in the tissue sample from the patient is 6.4 times or greater than the average level of expression of EDI-3 in human endometrial carcinoma tissue from patients with non-metastasizing endometrial carcinoma.