Patent ID: 6469151
Filing Date: 2002-10-22
Classification: C12Q

Abstract:
An array of PNA-DNA chimeric probes comprising a plurality of PNA-DNA chimeric probes immobilized on a solid substrate where a chimeric probe of known sequence occupies a defined area and said chimeric probes have the formula:Px-L-Ny wherein:Px is a PNA moiety and each P is independently a PNA monomer; x is an integer from 3 to 15; Ny is a DNA moiety and each N is independently a nucleotide; y is an integer of 2 or more; and L represents a covalent linkage between P and N; with the proviso that the terminal nucleotide N has a 3â€²-hydroxyl or phosphate group or a 5â€²-hydroxyl or phosphate group, whereby the DNA moiety is capable of ligation; and wherein the chimeric probe is covalently attached by a linker at a terminus of the PNA moiety to the solid substrate.