Patent ID: 6897300
Filing Date: 2005-05-24
Classification: C12N,Y02A

Abstract:
1. An isolated nucleic acid comprising: (a) a nucleotide sequence encoding an amino acid sequence represented by SEQ ID NO: 2 or 4, wherein said amino acid sequence having nicotianamine aminotransferase activity, or (b) a nucleotide sequence which hybridizes to the nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 3 when incubated in a solution of 5× Denhart's solution, 5×SSPE and 0.1% SDS at 65° C. for 12 hours, washed once with 6×SSP at 65° C. for 10 minutes and washed twice with 2×SSP, 0.1% SDS at 42° C. for 10 minutes, and which comprises a DNA sequence that is amplifiable by polymerase chain reaction with the primers represented by SEQ ID NO: 5 and 6 repeating a cycle of incubation at 94° C. for 40 seconds, followed by 40° C. for 1 minute, and followed by 72° C. for 2 minutes 25 times, and then repeating a cycle of incubation at 94° C. for 40 seconds, followed by 45° C. for 1 minute, and followed by 72° C. for 2 minutes 25 times, wherein the coding sequence of said nucleotide sequence encodes an amino acid sequence having nicotianamine aminotransferase activity.