Patent ID: 8546101
Filing Date: 2013-10-01
Classification: A61K,A61P,C12N

Abstract:
1. A method of screening a compound for an effect on cultured chondrocytes, comprising: a) providing primate pluripotent stem cells which express SSEA3, SSEA4, Tra-1-60 and Tra-1-81; b) culturing the primate pluripotent stem cells which express SSEA3, SSEA4, Tra-1-60 and Tra-1-81 in a medium comprising one or more of transforming growth factor (TGF), fibroblast growth factor (FGF), growth differentiation factor (GDF), bone morphogenic protein (BMP), hedgehog protein, L-ascorbic acid and para-thyroid hormone related protein to produce cultured chondrocytes; c) contacting the cultured chondrocytes with the compound; and d) observing a change in the cultured chondrocytes.