Patent ID: 8802828
Filing Date: 2014-08-12
Classification: C07K

Abstract:
1. A method for refolding a sample of abatacept, comprising the steps of: (a) obtaining an abatacept sample comprising a solution of abatacept exposed to a pH of about 3 and containing from 15% to 21% acid-induced aggregates; (b) adjusting the of the solution to above 5; (c) exposing the abatacept to an increased hydrostatic pressure of about 1000 bar to about 3000 bar for a period of time; and (d) reducing the hydrostatic pressure to atmospheric pressure; wherein the sample of abatacept after exposure to increased hydrostatic pressure has less than about 1% abatacept aggregates than the abatacept sample prior to the increased pressure exposure.