Patent ID: 6586243
Filing Date: 2003-07-01
Classification: A61K,C12N

Abstract:
A method for producing a disease-specific immunogenic cell composition capable of stimulating an immune response to said disease, the method comprising the steps of:a. isolating CD34 negative hematopoietic stem cells from a donor; b. incubating the stem cells in a cell culture medium comprising GM-CSF, SCF and IL-4, whereby the stem cells are directed to develop into programmable antigen presenting cells (pAPCs); c. mixing the pAPCs with cellular material derived from cells positive for said disease, the cellular material selected from the group consisting of cell lysate, cell RNA, cell poly A+ RNA, cDNA derived from cell RNA, and cDNA derived from cell RNA incorporated into an expression vector; d. incubating the pAPCs with the cellular material, whereby loaded pAPCs are produced; and e. incubating the loaded pAPCs with TNF-&agr;, whereby disease-specific programmed super antigen presenting cells (pSAPCs) capable of stimulating an immune response to said disease are produced.