Patent ID: 8242244
Filing Date: 2012-08-14
Classification: C07K,C12N,C12P

Abstract:
1. A composition comprising an orthogonal aminoacyl tRNA synthetase (O-RS) encoded by a nucleic acid comprising a polynucleotide sequence selected from the group consisting of: SEQ ID NO: 1, a sequence at least 80% identical to SEQ ID NO: 1 and encoding a polypeptide having at least 90% identity to the sequence of SEQ ID NO: 2 comprising a proline residue at a position corresponding to position 144 of SEQ ID NO: 2, or comprising a complementary polynucleotide sequence thereof; wherein the O-RS preferentially aminoacylates a reference O-tRNA of SEQ ID NO: 3 with 5-hydroxy-L-tryptophan (5-HTPP) or 5-substituted tryptophan analog when the reference O-tRNA 5-hydroxy-L-tryptophan (5-HTPP) or 5-substituted tryptophan analog, and the O-RS are present in a eukaryotic cell.