Patent ID: 8236489
Filing Date: 2012-08-07
Classification: C12Q,G01N

Abstract:
1. A method for testing sensitivity of a tumor to a therapeutic agent, the method comprising: mincing an epithelial tumor specimen to prepare a plurality of multicellular tissue explants, and placing the explants in liquid medium in one or more containers; physically agitating the explants, culturing said explants to form one or more non-confluent monolayers, and suspending the non-confluent monolayer(s) in culture medium to obtain from about 4,000 to about 12,000 cells per ml; transferring the cells to a plurality of wells at about 100 to about 1000 cells per well; adding a serial dilution of at least one therapeutic agent or combination across the wells, wherein the therapeutic agent comprises a monoclonal antibody; and determining sensitivity of the cells to the therapeutic agent.