Patent ID: 9121056
Filing Date: 2015-09-01
Classification: C12Q

Abstract:
1. A method for the detection of a target nucleic acid in a sample, comprising: amplifying, in a reaction mixture, a target nucleic acid using PCR in the presence of a pair of oligonucleotide primers wherein at least one of the oligonucleotide primers is designed to incorporate a 5′-specialty sequence to provide an amplification product that intramolecularly folds into a secondary structure; and detecting the amplification product that folds into a secondary structure by a method comprising: providing an oligonucleotide cleavage component that has no or reduced priming ability, hybridizing said oligonucleotide cleavage component with the amplification product to form a three-strand cleavage structure wherein two strands of the three-strand cleavage structure are provided by the secondary structure of the amplification product, cleaving 3′- or 5′-strands of the three-strand cleavage structure using a duplex-specific nuclease activity resulting in a cleavage product, and detecting the cleavage product, wherein the presence of the cleavage product is indicative of the presence of the target nucleic acid in said sample.