Patent ID: 6391546
Filing Date: 2002-05-21
Classification: C12Q

Abstract:
A method for detecting a target nucleotide sequence that comprises:converting a target nucleotide sequence to a partially double-stranded nucleotide sequence which has a double-stranded part and a single-stranded part; and detecting the partially double-stranded nucleotide sequence using a nucleotide sequence that is complementary to the target nucleotide sequence, wherein converting the target nucleotide sequence into the partially double-stranded nucleotide sequence includes: (1) performing an asymmetric polymerase chain reaction in which a target nucleotide sequence is used as a template and an oligonucleotide having a nucleotide sequence identical to a part of the target nucleotide sequence (primer 1) and an oligonucleotide having a nucleotide sequence complementary to a part of the target nucleotide sequence (primer 2) are used as primers; (2) performing an asymmetric polymerase chain reaction in which the target nucleotide sequence is used as a template and primer 1 and an oligonucleotide having a nucleotide sequence complementary to a part of the target nucleotide sequence except for primer 2 (primer 3) are used as primers; and (3) obtaining a partially double-stranded nucleotide sequence by heating and cooling a mixture of the amplification products of (1) and (2).