Patent ID: 9012134
Filing Date: 2015-04-21
Classification: A61K,A61P,C12N,G01N

Abstract:
1. A method of enriching a sample comprising T-cells for FoxP3 + immunosuppressive regulatory T-cells, the method comprising: providing the sample comprising T-cells, wherein the sample is a peripheral blood mononuclear cell (PBMC) sample; contacting the sample with a fluorescently labeled monoclonal CD4 antibody and a fluorescently labeled monoclonal CD127 antibody; and sorting the cells according to the amount of the fluorescently labeled CD4 and CD127 antibodies bound to them, wherein the sorting step comprises identifying the cells having bright levels of the bound CD4 antibody and having reduced or no levels of the bound CD127 antibody as CD4 thereby forming a FoxP3