Patent ID: 7183094
Filing Date: 2007-02-27
Classification: C12N,C12P

Abstract:
1. A method for decreasing the antibiotic-resistance of pathogenic acyl glucosaminyl inositol amidase-producing actinomycetes, wherein the first 20 amino acids from the N-terminus of a mycothiol S-conjugate amidase comprise an amino acid sequence having at least 80% sequence identity to SEQ ID NO: 2, said method comprising: contacting the actinomycetes with an inhibitor of acyl glucosaminyl inositol amidase activity, wherein the inhibitor is mycothiol, wherein the intracellular presence of the inhibitor decreases activity of the amidase, thereby decreasing the antibiotic resistance of the actinomycetes as compared with untreated controls, and wherein when the actinomycetes is contacted with 3-(bromomethyl)-2,5,6-trimethyl-1H,7H-pyrazolo[1,2-a]pyrazole-1,7-dione (monobromobimane [mBBr]) in the absence of the inhibitor, the actinomycetes converts mBBr into a mercapturic acid, which acid is exported from the actinomycetes.