Patent ID: 6100057
Filing Date: 2000-08-08
Classification: C07K,C12N

Abstract:
A method for producing a secreted hemoprotein, comprising:(a) introducing into a filamentous fungal cell,(i) one or more first control sequences, wherein the one or more first control sequences, after introduction, are operably linked to one or more first nucleic acid sequences encoding one or more heme biosynthetic enzymes, wherein the first nucleic acid sequences are endogenous to the filamentous fungal cell; and/or(ii) one or more copies of one or more second nucleic acid sequences encoding one or more heme biosynthetic enzymes operably linked to one or more second control sequences which direct the expression of the second nucleic acid sequences;(b) cultivating the filamentous fungal cell in a nutrient medium suitable for production of the secreted hemoprotein and the heme biosynthetic enzymes; and(c) recovering the secreted hemoprotein from the nutrient medium of the filamentous fungal cell.