Patent ID: 8623617
Filing Date: 2014-01-07
Classification: A61P,C12N,C12Q

Abstract:
1. A method for determining a treatment regimen for a subject with a breast condition, comprising: extracting RNA from cells obtained from a breast of the subject to produce an extracted RNA sample; performing an assay to determine an expression level of a Paired Box 2 (PAX2) gene relative to an expression level of a control gene in the extracted RNA sample; performing an assay to determine an expression level of a beta defensin-1 (DEFB1) gene relative to the expression level of the control gene in the extracted RNA sample; and determining a PAX2-to-DEFB1 expression ratio based on the expression levels of PAX2 and DEFB1 in the extracted RNA sample, wherein the expression levels of PAX2, DEFB1 and the control genes are determined by quantitative real-time RT-PCR; determining a treatment regimen for said subject based on the PAX 2-to-DEFB1 expression ratio, wherein a PAX 2-to-DEFB1 expression ratio of 100:1 or higher is indicative of the presence of breast cancer in the subject, and a PAX2-to-DEFB1 expression ratio of less than 100:1 is indicative of the presence of non-cancerous or pre-cancerous breast condition in the subject.