Patent ID: 7556921
Filing Date: 2009-07-07
Classification: C12Q,G01N

Abstract:
1. A method of identifying at least one gene that is regulated by a signaling pathway that comprises at least one polypeptide, wherein said polypeptide is not a known DNA binding polypeptide and is not predicted to bind DNA because it lacks a known DNA binding domain, said method comprising: (a) producing a mixture comprising DNA fragments to which said polypeptide is bound in a cell; (b) isolating one or more DNA fragments to which said polypeptide is bound from the mixture produced in step (a), wherein said isolating is carried out by using an agent that specifically binds to said polypeptide; (c) identifying regions of the genome which are complementary to the DNA fragments isolated in step (b); and (d) identifying at least one gene operably-linked to a region of the genome identified in step (c) to identify at least one gene that is regulated by said signaling pathway.