Patent ID: 8748103
Filing Date: 2014-06-10
Classification: C12Q

Abstract:
1. A method of monitoring a lymphoid neoplasm in a patient by one or more patient-specific clonotypes correlated with the lymphoid neoplasm, the method comprising the steps of: (a) obtaining a sample from the patient comprising T-cells and/or B-cells; (b) amplifying molecules of nucleic acid from the T-cells and/or B-cells of the sample, the molecules of nucleic acid comprising recombined DNA sequences from T-cell receptor genes or immunoglobulin genes; (c) sequencing the amplified molecules of nucleic acid to form a clonotype profile, wherein the sequencing comprises at least 10,000 reads per run; and (d) determining from the clonotype profile a presence, absence and/or level of the one or more patient-specific clonotypes correlated with the lymphoid neoplasm and phylogenic clonotypes thereof.