Patent ID: 8990024
Filing Date: 2015-03-24
Classification: G01N

Abstract:
1. A fluorescence intensity compensation method, comprising: receiving, with photodetectors having different input wavelength bands, fluorescence emitted from fluorochromes excited by irradiating light on microparticles multiply-labeled by a plurality of fluorochromes with overlapping fluorescence wavelengths; collecting detected values for the photodetectors; and obtaining a measurement spectrum, by approximating, from a linear sum of single-stain spectrums obtained from microparticles individually labeled with the fluorochromes; wherein approximation of said measurement spectrum by the linear sum of said single-stain spectrums is performed using a restricted least-square method; wherein the intensity of fluorescence emitted from each fluorochrome is calculated by obtaining a parameter x where S ij represents a detected value of the i'th photodetector in the single-stain spectrum of the j'th fluorochrome, p i represents a detected value of the i'th photodetector in the measurement spectrum, σ i represents an inverse number of a weight as to a detected value of the i'th photodetector, and U j represents a lower limit value of the fluorescence intensity of each fluorochrome to be calculated; wherein said parameter x where S represents an N×M order matrix with s ij as elements, x represents an M order matrix with x j as elements, p represents an N'th order vector with p i as elements, A is set as an M×M order matrix and b as an M×1 order matrix, and U 1 through U M represent said lower limit value; wherein in said Expression (2), an undyed average value V