Patent ID: 9145574
Filing Date: 2015-09-29
Classification: C12Q,G01N

Abstract:
1. A method of mapping the spatial distribution of one or more markers associated with microorganism(s) and/or biofilm(s) in a wound comprising: (a) contacting an unstained membrane with the wound, wherein the membrane is positively charged and adsorbs negatively charged biological molecules when contacted with the wound; (b) blocking the membrane; (c) applying a stain to the blocked membrane and adsorbed biological molecules; (d) rinsing the membrane; and (e) analyzing the dye stained membrane to determine the spatial distribution of said markers of microorganism(s) and/or biofilm(s) of interest in the wound: wherein the stain targets one or more markers associated with microorganism(s) and/or biofilm(s) interest in the wound, wherein the stain comprises a plurality of detection ligand molecules that are specific to one or more negatively charged components found in extracellular matrix of the microbial biofilm produced by certain microbial class(es), genera, and/or species, and wherein at least one of said plurality of detection ligand molecules is targeted to one or more of polyanionic bacterial exopolysaccharide, poly-β-(1-6)-N-acetyl-D-glucosamine, or alginic acid.