Patent ID: 8846307
Filing Date: 2014-09-30
Classification: C12M,C12N

Abstract:
1. A method for creating tissue structures in vitro comprising: juxtaposing a plurality of mandrels on a culture/perfusion device connecting the plurality of mandrels to a plurality of tubes including a plurality of upstream tubes and a plurality of downstream tubes, where each of the plurality of mandrels is held at an upstream end by one of the plurality of upstream tubes and at a downstream end by one of the plurality of downstream tubes; connecting the plurality of upstream tubes to an upstream manifold; combining the plurality of downstream tubes to a downstream manifold; seeding the plurality of mandrels with endothelial cells or smooth muscle cells that multiply and form circular layers around each of the mandrels; subjecting the mandrels and cells to a growth medium; adjusting the distance between the plurality of mandrels to within 100 micrometers to allow the cells to multiply and form circular layers around each of the plurality of mandrels until the circular layers merge into capillary-like tissue structure; then extracting the mandrels to form a plurality of channels in the capillary-like tissue structure; and then perfusing the capillary-like tissue structure with perfusate.