Patent ID: 6495320
Filing Date: 2002-12-17
Classification: C12Q

Abstract:
A method for the amplification of nucleic acid, said method comprising:randomly digesting a single-stranded DNA population into approximately equal length fragments by: contacting a single-stranded DNA population with an enzymatic compound comprising at least two 1,10-phenanthroline coppers attached to a non-specific nucleic acid binding element wherein said compound exhibits non-specific nucleic acid binding and binds to a fixed length segment of nucleic acid; initiating cleavage of the DNA at locations on either side of the bound fixed length segment, by the 1,10-phenanthroline coppers, to produce a plurality of single-stranded DNA fragments that are of approximately equal length; synthesizing double-stranded DNA from said single-stranded DNA fragments; and producing multiple copies of sense RNA from said double-stranded DNA.