Patent ID: 7416874
Filing Date: 2008-08-26
Classification: A01K,A21D,A23J,A23K,A23L,A23V,A61K,A61P,C12N,C12Y

Abstract:
1. An isolated, synthetic or recombinant nucleic acid encoding a polypeptide having phytase activity that is expressed and secreted in a yeast cell, wherein the nucleic acid is generated by a method comprising the following steps: (a) providing a nucleic acid comprising (i) the sequence of SEQ ID NO: 1, (ii) the sequence of SEQ ID NO: 1, wherein T can also be U, (iii) the sequence of a nucleic acid encoding a polypeptide comprising the sequence of SEQ ID NO: 2, (iv) the sequence of a nucleic acid encoding a polypeptide having the amino acid sequence of SEQ ID NO: 2 from amino acid residues 1 to 432, (v) the sequence of a nucleic acid encoding a polypeptide having the amino acid sequence of SEQ ID NO: 2 from amino acid residue 1 to 432 and a heterologous amino acid sequence; (vi) the sequence of SEQ ID NO: 1 from nucleotide residue 1 to 1296, or (vii) sequences completely complementary to (i), (ii), (iii), (iv), (v), or (vi); (b) modifying one or more nucleotides in the nucleic acid to another nucleotide, deleting one or more nucleotides in the nucleic acid, or adding one or more nucleotides to the nucleic acid, wherein the modified nucleic acid encodes a polypeptide having phytase activity and the modified nucleic acid hybridizes under stringent hybridization conditions to the nucleic acid of SEQ ID NO: 1, wherein the hybridization occurs because the modified nucleic acid has at least 95% sequence identity to SEQ ID NO: 1, and all the modifications result in a conservative amino acid substitution of one amino acid for another amino acid; and (c) adding to the nucleic acid of (b) a nucleic acid encoding a yeast secretory signal peptide or transit peptide; wherein expressing the nucleic acid in a yeast cell generates a secreted polypeptide having phytase activity.