Patent ID: 6270965
Filing Date: 2001-08-07
Classification: C12Q

Abstract:
A method for detection of a nucleic acid comprising:(a) providing a sample in which it is intended to detect a nucleic acid,(b) amplifying the nucleic acid in a closed device in the presence of at least one nucleic acid primer which is specific for the nucleic acid to be detected and at least one capture probe which is specific for the nucleic acid to be detected, the capture probe being immobilized on at least one inner surface of the device and selected in such a way that a hybrid of nucleic acid and capture probe has a lower melting temperature than a hybrid of nucleic acid and primer and wherein the amplification is carried out under such conditions that a hybrid of nucleic acid and primer is stable whereas a hybrid of nucleic acid and capture probe is unstable, thereby forming an amplification product,(c) binding the amplification product to the immobilized capture probe, and(d) detecting the binding of the amplification product to the capture probe by evanescence-induced fluorescence,wherein said closed device is closed during the entire amplifying and detecting procedure.