Patent ID: 6194221
Filing Date: 2001-02-27
Classification: G01N,Y10S

Abstract:
An immunochromatographic assay device for detection of the presence or absence of an analyte in a liquid sample, wherein said immunochromatographic assay device comprises:(a) a sample receiving region comprising a porous material which conducts lateral flow of a liquid sample, in lateral flow contact with(b) an analyte detection region comprising a porous material which conducts lateral flow of said liquid sample, wherein said analyte detection region comprises an immobile indicator capture reagent at a discrete indicator capture reagent situs,wherein said immunochromatograplic device also comprises:a first indicator labeling reagent zone comprising a first mobile indicator labeling reagent, and a second indicator labeling reagent zone comprising a second mobile indicator labeling reagent wherein the lateral flow characteristics of the indicator labeling reagent in the first zone differ from the lateral flow characteristics of the indicator labeling reagent in the second zone, wherein said first mobile indicator labeling reagent forms a complex binding with said analyte, and wherein said second mobile indicator labeling reagent forms a complex binding with said analyte, and wherein said zones are in lateral flow contact with said sample receiving region and said analyte detection region, and wherein said liquid sample laterally flows from said sample receiving region towards said analyte detection region, and mixes with said first and second indicator labeling reagents to move said first and second indicator labeling reagents towards said analyte detection region, wherein said immobile indicator capture reagent forms a complex comprising said analyte, said first or second mobile indicator labeling reagent and said immobile indicator capture reagent.