Patent ID: 7138565
Filing Date: 2006-11-21
Classification: C12N,C12Y

Abstract:
1. A method for reducing the phenotypic expression of a nucleic acid of interest, which is normally capable of being expressed in a plant cell, said method comprising the steps of a) providing to the nucleus of said plant cell unpolyadenylated RNA comprising a target specific nucleotide sequence, produced by transcription of a chimeric DNA comprised within said plant cell, said chimeric DNA comprising a plant-expressible promoter operably linked to a target specific DNA region encoding said RNA, said target-specific DNA region comprising a nucleotide sequence of at least 20 consecutive nucleotides having 100% sequence identity to a nucleotide sequence of at least 20 consecutive nucleotides from the nucleotide sequence of said nucleic acid of interest or to the complementary sequence of said nucleic acid of interest; and b) selecting said plant cell wherein the phenotypic expression of said nucleic acid of interest is reduced.