Patent ID: 6194208
Filing Date: 2001-02-27
Classification: A23J,A23V,A61K,A61P,C07K,C12N

Abstract:
A process for preparing a growth extract, which process comprises:(a) isolating a milk protein mixture from a milk product selected from the group consisting of cheese whey, skim milk, and casein whey, the isolated milk protein mixture comprising growth factors having a basic to approximately neutral isoelectric point, being enriched in growth promoting activity, and having the ability to stimulate proliferation of rat myoblasts, the milk protein mixture being isolated by ion exchange chromatography without exposure to acidic conditions such that the milk protein mixture elutes during the chromatography in a fraction having a high molecular weight; and(b) subjecting the isolated milk protein mixture to transient acidification with an acid source to obtain an acidified growth promoting extract with a cell growth promoting activity that is enhanced as compared with the cell growth promoting activity of the milk protein mixture isolated in step (a).