Patent ID: 6902891
Filing Date: 2005-06-07
Classification: C07H,C12Q

Abstract:
1. A process for fragmenting and labeling at least one synthetic or natural DNA, RNA or chimeric DNA-RNA polymer, comprising the steps of: obtaining a mixture in which the at least one DNA, RNA or chimeric DNA-RNA polymer has been subject to an in vitro nucleic acid amplification reaction; chemically fragmenting the at least one DNA, RNA or chimeric DNA RNA polymer in the mixture in the presence of at least one multivalent metal cation in an aqueous solution, to produce a plurality of DNA or RNA fragments having freed terminal phosphates for further reaction; and attaching a labeling agent on a plurality of said fragments at freed terminal phosphates located at the 3′ end and/or 5′ end of said fragments, wherein the fragmenting and attaching steps take place in an in vitro nucleic acid amplification mixture.