Patent ID: 7410801
Filing Date: 2008-08-12
Classification: C07H,C12N,C40B

Abstract:
1. A process of obtaining recombinant lambdoid bacteriophage with high density display of functional peptides and proteins on surface of said bacteriophage comprising the steps of: a) Constructing a donor plasmid comprising: wherein each translatable nucleotide sequence is in the same reading frame such that induction of said first cistron expresses a fusion polypeptide consisting of said capsid polypeptide, linker polypeptide, protease cleavage site, linker polypeptide, and said MCS-encoded or said insert-encoded polypeptide; a second inducible cistron for expression of a second selectable marker transferring the polynucleotide sequences located between said d) propagating said cointegrates in liquid medium selective for said first selectable marker; and e) harvesting phages displaying said fusion polypeptide.