Patent ID: 7871764
Filing Date: 2011-01-18
Classification: C12N,C12Q

Abstract:
1. A method for extracting nucleic acids from a biological sample comprising: providing a biological sample containing nucleic acids; applying the biological sample to a column; after applying the sample to the column, treating the column with a first protease containing mixture and allowing the first protease treated column to incubate; after treating the column with a first protease, centrifuging the column collecting the flow-through formed thereform and transferring the flow-through into an ethanol solution, forming a first collected flow-through; after collecting the first flow-through, washing the column with a first alcohol solution, centrifuging the column and discarding the flow-through formed therefrom; after washing the column with a first alcohol solution, treating the column with a lysozyme containing mixture and allowing the lysozyme treated column to incubate; after treating the column with a lysozyme containing mixture, centrifuging the column, collecting the flow-through formed therefrom and transferring the flow-through into an ethanol solution, forming a second collected flow-through; after collecting the second flow-through washing the column with a second alcohol solution, centrifuging the column and discarding the flow-through formed therefrom, after the magnesium free L:B treatment, adding a MgCl after adding the MgCl after washing the column with the first, second, and third collected flow-throughs, adding an activated L:B buffer to the column to bind the released nucleic acids to the column, and then centrifuging the column; and eluting the nucleic acids to provide extracted nucleic acids.