Patent ID: 9169486
Filing Date: 2015-10-27
Classification: C12N,C12P

Abstract:
1. A non-naturally occurring microbial organism, said microbial organism having a butadiene pathway and comprising at least two exogenous nucleic acids each encoding a butadiene pathway enzyme expressed in a sufficient amount to produce butadiene, wherein said butadiene pathway comprises the butadiene pathway enzymes of an acetyl-CoA:acetyl-CoA acyltransferase, an acetoacetyl-CoA reductase, a 3-hydroxybutyryl-CoA dehydratase, a crotonyl-CoA reductase (aldehyde forming), a crotonaldehyde reductase (alcohol forming), a crotyl alcohol kinase, a 2-butenyl-4-phosphate kinase and a butadiene synthase; said microbial organism further comprising: (a) a reductive TCA pathway comprising at least one exogenous nucleic acid encoding a citryl-CoA synthetase or a citryl-CoA lyase; or (b) a reductive TCA pathway comprising at least one exogenous nucleic acid encoding a phosphoenolpyruvate carboxylase or a phosphoenolpyruvate carboxykinase.