Patent ID: 9109261
Filing Date: 2015-08-18
Classification: C12Q

Abstract:
1. A device for detecting nucleic acids encoding resistance to an antibiotic, comprising a solid support and a plurality of detectably labeled solution-phase hybridization probes distributed among a plurality of loci thereon, said plurality of loci comprising, (a) a first locus that comprises one or more solution-phase hybridization probes that collectively hybridize to ribosomal nucleic acids from a plurality of species of bacteria in the genus (b) a second locus that comprises a probe mix containing (c) a third locus that comprises a probe mix containing a first mecA solution-phase hybridization probe that consists of the nucleotide sequence of SEQ ID NO:2 or the complement thereof, a second mecA solution-phase hybridization probe that consists of the nucleotide sequence of SEQ ID NO:3 or the complement thereof, and a third mecA solution-phase hybridization probe that consists of the nucleotide sequence of SEQ ID NO:5 or the complement thereof, and (d) a fourth locus that comprises a solution-phase hybridization probe that hybridizes both to VanA nucleic acids and VanB nucleic acids, wherein said solution-phase hybridization probe consists of the nucleotide sequence of SEQ ID NO:33 or the complement thereof; wherein each of said plurality of loci is configured as a matrix that provides an identification of one or more unknown nucleic acids from a sample, and wherein each of said detectably labeled solution-phase hybridization probes comprises a polynucleotide sequence which is covalently attached to a non-nucleotide detectable moiety that emits a detectable signal.