Patent ID: 6828129
Filing Date: 2004-12-07
Classification: C12N,C12P

Abstract:
An isolated polynucleotide comprising any one of the following nucleotide sequences:(a) the nucleotide sequence represented by SEQ ID NO:2; (b) a nucleotide sequence which encodes an amino acid sequence of a protein capable of hydrolyzing a racemic ethyl N-benzylazetidine-2-carboxylate asymmetrically and producing an (S)-N-benzylazetidine-2-carboxylic acid and which consists of a part of the nucleotide sequence of SEQ ID NO:2; (c) a nucleotide sequence which encodes an amino acid sequence of a protein capable of hydrolyzing a racemic ethyl N-benzylazetidine-2-carboxylate asymmetrically and producing an (S)-N-benzylazetidine-2-carboxylic acid and which hybridizes under stringent condition of between 450 and 900 mM sodium chloride, 45 to 90 mM sodium citrate and a temperature of 65Â° C., with the complement of a DNA sequence consisting of a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:1; and, (d) a nucleotide sequence of a DNA molecule which is amplified by a PCR using as primers oligonucleotides that provide for amplification of a DNA molecule having a nucleotide sequence represented by nucleotides 235 to 765 of SEQ ID NO:2, and as a template a chromosome DNA molecule derived from a microorganism belong to Aspergillus flavus.