Patent ID: 6143507
Filing Date: 2000-11-07
Classification: G01N,Y10S

Abstract:
A quantitative assay for measuring the ability of an antagonist or agonist to effect binding of a TRAF protein or fragment thereof to a TRAF interacting receptor having a cytosolic binding domain or fragment thereof, the TRAF protein or fragment thereof comprising SEQ ID NO: 1 residues 1-240 or SEQ ID NO:2 residues 1-225, the assay comprising:providing the receptor having a cytosolic binding domain or fragment thereof, wherein the receptor is bound to a multi-well plate, the multi-well plate allowing formation of a multimeric receptor and allowing detection of a signal; providing the TRAF protein or fragment thereof possessing a terminal tag, the tag comprising one or more amino acids and allowing binding a signal-generating antibody or fragment thereof; providing the antagonist or agonist; combining the receptor bound to the well, the TRAF protein and the antagonist or agonist; detecting the signal-generating antibody and measuring the inhibition or enhancement of the antagonist or agonist.