Patent ID: 6548287
Filing Date: 2003-04-15
Classification: A61K,A61P,C12N,C12P,C12R

Abstract:
A method for preparing a culture comprising a non-pyrogenic bacterial vaccine, said method comprising the steps of:(a) genetically modifying a gram-negative host bacterial strain that is suitable for use as a vaccine, said step of genetically modifying comprising introducing a conditional mutation in one or more genes selected from the group consisting of kdsA, kdsB, kdtA, lpxA, lpxB, lpxC, lpxD, ssc, pmr, htrB and msbB of the gram-negative host strain, wherein the conditional mutation affects the biosynthesis of lipid A under non-permissive culture conditions, and results in the synthesis of non-pyrogenic lipid A; and (b) culturing the genetically modified gram-negative host strain in non-permissive culture conditions to produce non-pyrogenic lipid A thereby generating a non-pyrogenic vaccine.