Patent ID: 8841085
Filing Date: 2014-09-23
Classification: C12Q,G01N,Y10S

Abstract:
1. A nanoparticle sensor for measuring protease activity, in which a fluorophore and a quencher are conjugated to a peptide substrate, and the peptide substrate is covalently conjugated to a biocompatible polymer nanoparticle, the peptide substrate being specifically lysed by protease, wherein the protease is selected from a group consisting of matrix metalloproteinases (MMPs); the peptide substrate is selected from a group consisting of Gly-Pro-Leu-Gly-Val-Arg-Gly-Lys-Gly-Gly (SEQ ID NO: 1), Gly-Val-Pro-Leu-Ser-Leu-Thr-Met-Gly-Lys-Gly-Gly (SEQ ID NO: 2) and Gly-Pro-Leu-Gly-Met-Arg-Gly-Leu-Gly-Lys-Gly-Gly (SEQ ID NO: 3); and the biocompatible polymer nanoparticle is a hydrophobic modified glycol chitosan.