Patent ID: 8273558
Filing Date: 2012-09-25
Classification: C12P,Y02E

Abstract:
1. A recombinant yeast host cell comprising genes encoding an engineered isobutanol biosynthetic pathway, wherein said isobutanol biosynthetic pathway increases the production of isobutanol as compared to naturally occurring amounts of isobutanol made by unmodified yeast and comprises the following substrate to product conversions: (a) 2,3-dihydroxy-isovalerate to α-ketoisovalerate; (b) the α-ketoisovalerate from (a) to isobutyraldehyde; and (c) the isobutyraldehyde from (b) to isobutanol, wherein (ii) substrate to product conversion of step (b) is performed by a recombinantly expressed decarboxylase enzyme; and (iii) the substrate to product conversion of step (c) is performed by a recombinantly expressed alcohol dehydrogenase enzyme, wherein the recombinantly expressed enzymes of (i) to (iii) are expressed in the cytosol, and wherein said recombinant yeast host cell is capable of producing isobutanol through the substrate to product conversions of (a) to (c).