Patent ID: 8945862
Filing Date: 2015-02-03
Classification: A61K,C12N

Abstract:
1. A method of monitoring human embryonic stem cells (hESCs) comprising: providing human embryonic stem cells; transducing the hESCs with a triple-fusion expression vector to produce triple-fusion hESCs, wherein the triple-fusion expression vector comprises a firefly luciferase reporter gene (Fluc) having a nucleotide sequence selected from the group consisting of: SEQ ID NO: 1 and SEQ ID NO: 3, a red fluorescent reporter gene (RFP) having a nucleotide sequence of SEQ ID NO: 6, and a truncated HSV1-tK positron emission tomography (PET) reporter gene (HSV1-tTK) having a nucleotide sequence selected from the group consisting of: SEQ ID NO: 7 and SEQ ID NO: 14; and detecting fluorescence, bioluminescence, a nuclear signal from a PET reporter, or a combination thereof, emitted from the triple-fusion hESCs and any progeny of the triple-fusion hESCs.