Patent ID: 9175295
Filing Date: 2015-11-03
Classification: A01K,C12N

Abstract:
1. A modified transposon vector, comprising a nucleic acid fragment having the following features (a)-(b) or (a)-(c): (a) 5′- and 3′-terminal inverted repeat (TIR) sequences of a transposon gene; (b) a Lox sequence where a recombination reaction occurs is disposed between the two direct repeats (DR) of at least one of the DR regions present in each of the 5′- and 3′-TIR sequences of (a) such that transposon activity is retained, wherein the number of nucleotides between the two DRs is the same as that before said Lox is disposed between the two DRs, and wherein said Lox allows for destruction of the transposon activity through deletion, insertion or replacement reaction to prevent re-transposition from occurring; and (c) a restriction enzyme recognition site or an expression cassette of a foreign gene disposed between the 5′- and 3′-TIR sequences of (a).