Patent ID: 6890750
Filing Date: 2005-05-10
Classification: C07H,C12N,C12Q

Abstract:
1. A cell line comprising a stably integrated recombinant nucleic acid construct comprising: a reporter gene operably linked to a recognition sequence for a sequence-specific DNA-binding protein: and a stably integrated recombinant nucleic acid construct comprising a sequence encoding a fusion protein, said fusion protein comprising a sequence-specific DNA binding domain, wherein said DNA binding domain specifically binds said recognition sequence, and a conditionally active transactivation domain of CHOP, wherein bindings of said fusion protein to said recognition sequence results in transactivation of said reporter gene when said transactivation domain fused to said DNA binding domain is activated, and wherein said sequence-specific DNA binding domain of said fusion protein is located upstream of said conditionally active transactivation domain of CHOP, wherein said recognition sequence for a sequence-specific DNA-binding domain is that sequence recognized by one of the group consisting of GAL4 and LexA.