Patent ID: 6461818
Filing Date: 2002-10-08
Classification: A01K,C12N

Abstract:
A method for deleting a selected region of genetic material in mice comprising the steps of:inserting a first selection cassette at a 5â€² end of said selected region using conventional gene targeting methods, said first selection cassette comprising a first selectable marker coding sequence, a first loxP recombination site, and a first portion of a second selectable marker coding sequence; selecting embryonic stem cells expressing said first selectable marker coding sequence; inserting a second selection cassette at a 3â€² end of said selected region using conventional gene targeting methods, said second selection cassette comprising a third selectable marker coding sequence, a second loxP recombination site, and a remaining portion of said second selectable marker coding sequence; selecting embryonic stem cells expressing said third selectable marker coding sequence; expressing Cre recombinase to produce recombination between said first and second loxP sites; selecting embryonic stem cells expressing said second selectable marker coding sequence, wherein the selected region of genetic material is deleted; injecting selected embryonic stem cells expressing said second marker into a recipient blastocyst; and implanting said blastocyst into a foster mother.