Patent ID: 8617895
Filing Date: 2013-12-31
Classification: G01N,Y10T

Abstract:
1. A method for preparing a coated substrate adapted for immobilizing a biological sample, said method comprising: providing a glass substrate having a surface comprising a plurality of anionic groups; contacting the substrate with a composition comprising a solution of a non-peptidic, quaternary ammonium polymeric material comprising at least one monomer selected from the group consisting of diallyldimethylammonium, methylacrylamidopropyltrimethylammonium, methacryloyloxyethyltrimethylammonium, 4-vinyl-benzyltrimethylammonium, acryloxyethyldimethylbenzyl ammonium, acryloxyethyltrimethyl ammonium, methacryloxyethyldimethylammonium, methacryloxyethyltrimethylbenzylammonium, trimethyl-2-methacryloylethylammonium, trimethyl-2-methacrylaminopropylammonium, and mixtures thereof, said quaternary ammonium polymeric material present in a concentration of about 0.01% (w/v) to about 10.0% (w/v) in solution, the solution having a pH of about 8 to about 14, to form a coating of a single layer of the non-peptidic, quaternary ammonium polymeric material on at least a portion of the surface of the substrate with no intervening layers of a different coating material sandwiched between two or more layers of the non-peptidic, quaternary ammonium polymeric coating material and with no further layers of any material coated over said layer of the non-peptidic, quaternary ammonium polymeric material; wherein the non-peptidic, quaternary ammonium polymeric coating material has an average molecular weight of about 400,000 Da to about 500,000 Da, and drying the coating of the non-peptidic, quaternary ammonium polymeric material.