Patent ID: 8026352
Filing Date: 2011-09-27
Classification: C12N,C12Q

Abstract:
1. A kit for identifying elite event MS-BN1 in a biological sample of a Brassica plant, Brassica plant material, or a product comprising a Brassica plant material, said kit comprising at least two primers, wherein: (a) a first primer comprises 19 consecutive nucleotides of nucleotides: 1-867 of SEQ ID NO: 13; or nucleotides 1-771 of SEQ ID NO: 36; and a second primer comprises 19 consecutive nucleotides of the complement of: nucleotides 868-953 of SEQ ID NO: 13 or of nucleotides 772-909 of SEQ ID NO: 36, or wherein a second primer comprises 19 consecutive nucleotides of SEQ ID NO: 1 or the complement thereof; or (b) a first primer comprises 19 consecutive nucleotides of the complement of nucleotides 181-537 of SEQ ID NO: 18 or nucleotides 181-522 of SEQ ID NO: 38; and a second primer comprises 19 consecutive nucleotides of nucleotides 1-180 of SEQ ID NO: 18 or nucleotides 1-180 of SEQ ID NO: 38, or wherein a second primer comprises 19 consecutive nucleotides of SEQ ID NO: 1, or the complement thereof; wherein the first and the second primer amplify a DNA fragment from a nucleic acid present in a biological sample that comprises elite event MS-BN1, and wherein the first and the second primer do not amplify said DNA fragment from a nucleic acid present in a biological sample that does not comprise elite event MS-BN1.