Patent ID: 6485983
Filing Date: 2002-11-26
Classification: G01N,Y10S

Abstract:
A system suitable for concurrent electrochemical, quantitative determination of multiple analytes, in a common test fluid sample, utilizing stripping voltammetry, said system consisting essentially of:A. at least one test kit reagent comprising an electrochemically active labeled substance that is specific for binding interaction with an analyte of interest or mimicking of an analyte of interest within said analyte determination system; B. a solid phase, porous, electrochromatographic medium having a common, linear fluid pathway defined therein, said linear pathway having a delimited area containing at least one immobilized binding material specific for binding to at least one labeled substance or to a complex of a labeled substance and an analyte suspected of being present in said common fluid test sample; C. a sample absorbing means, in fluid communication with said electrochromatographic medium, for combining said test kit reagent(s) and a fluid test sample suspected of containing one or more analytes of interest; D. electrode means coincident with and in spaced-apart relationship with said delimited area of said medium, said electrode means comprising an electrochemical cell including at least one working electrode and second electrode selected from the group consisting of a reference electrode and a counter electrode; E. means for pre-concentration of an electrochemically active label from said electrochemically active labeled substance by electrolytic displacement of said electrochemically active label from said labeled substance(s), and/or from said complex(es) of a labeled substance and analyte(s), within said delimited area of said medium, so as to cause said electrochemically active label to form a deposit upon said working electrode of said electrochemical cell; F. means for activation of said electrochemical cell so as to strip said label from said working electrode by stripping voltammetry and thereby produce a current signal response indicative of the concentration of said analyte(s) of said common test fluid; and G. means for monitoring of said electrochemical cell for a current signal resulting from said stripping voltammetry, and thereby correlating said current signal with the presence of said analyte(s) of interest.