Patent ID: 8197691
Filing Date: 2012-06-12
Classification: A61P,B01D,C11B,Y02E

Abstract:
1. A method of selectively removing products from an algal biomass comprising intact algal cells, the method comprising: a. combining an algal biomass and a first solvent set, to generate a first extraction mixture, the first extraction mixture including a first solid phase and a first liquid phase comprising proteins; b. separating at least a portion of the first liquid phase of the first extraction mixture from the first solid phase; c. combining the first extraction solid phase and a second solvent set, to generate a second extraction mixture, the second extraction mixture including a second solid phase and a second liquid phase comprising polar lipids, wherein the second extraction mixture is less polar than the first extraction mixture; d. separating at least a portion of the second liquid phase of the second extraction mixture from the second solid phase; e. combining the second extraction solid phase and a third solvent set, to generate a third extraction mixture, the third extraction mixture including a third solid phase and a third liquid phase comprising neutral lipids, wherein the third extraction mixture is less polar than the second extraction mixture wherein the first liquid phase has a higher protein concentration than the second and third liquid phases, the second liquid phase has a higher polar lipids concentration than the first and third liquid phases, and the third liquid phase has a higher neutral lipids concentration than the first and second liquid phases; and f. separating at least a portion of the third liquid phase of the third extraction mixture from the third solid phase.