Patent ID: 8597943
Filing Date: 2013-12-03
Classification: C12N

Abstract:
1. A method for the cultivation of a population of cells in a serum free cell culture medium, wherein the cells of the population of cells are cells which are able to grow in an animal component free medium and wherein the population of cells has a cell concentration of less than 100 cells/ml, comprising the steps of: a) culturing a population of cells at a cell concentration greater than 100 cells/ml in a first serum free cell culture medium; b) reducing the cell concentration to less than 100 cells/ml; c) culturing the cells in a second serum free cell culture medium, wherein the second serum free cell culture medium contains recombinant albumin and recombinant transferrin; and d) culturing the cells in a third serum free cell culture medium which has a lower concentration of recombinant transferrin and/or recombinant albumin than the cell culture medium of step c).