Patent ID: 8242329
Filing Date: 2012-08-14
Classification: C07K,C12N,C12P,Y02E

Abstract:
1. An isolated polynucleotide comprising a nucleotide sequence encoding a polypeptide having cellulolytic enhancing activity, selected from the group consisting of: (a) a polynucleotide encoding a polypeptide having cellulolytic enhancing activity comprising an amino acid sequence having at least 95% identity to amino acids 26 to 253 of SEQ ID NO: 2; (b) a polynucleotide encoding a polypeptide having cellulolytic enhancing activity that hybridizes under high stringency conditions with the full-length complement of nucleotides 76 to 832 of SEQ ID NO: 1, wherein high stringency conditions are defined as prehybridization and hybridization at 42° C. in 5×SSPE, 0.3% SDS, 200 micrograms/ml sheared and denatured salmon sperm DNA, and 50% formamide for 12 to 24 hours and washing three times each for 15 minutes using 2×SSC, 0.2% SDS at 65° C.; (c) a polynucleotide encoding a polypeptide having cellulolytic enhancing activity comprising amino acids 26 to 253 of SEQ ID NO: 2; (d) a polynucleotide encoding a polypeptide having cellulolytic enhancing activity comprising nucleotides 76 to 832 of SEQ ID NO: 1; and (e) a fragment of the polynucleotide of (a), (b), (c), or (d) encoding a polypeptide fragment having cellulolytic enhancing activity.