Patent ID: 6270961
Filing Date: 2001-08-07
Classification: B01J,C12Q,C40B,Y02P

Abstract:
A method of analyzing nucleic acids by hybridization, comprising the steps of:contacting a first plurality of different nucleic acid segments disposed on a first sector of a substrate with a first hybridization oligonucleotide, wherein said first plurality of nucleic acids is arranged on said first sector in a spatially identifiable array, under conditions that discriminate between a perfect match and a single base mismatch in the hybridization of a member of said first array of nucleic acid segments to the informational portion of said first hybridization oligonucleotide;contacting a second plurality of different nucleic acid segments disposed on a second sector of the substrate with a second hybridization oligonucleotide, wherein said second plurality of nucleic acids is arranged on said second sector in a spatially identifiable array, under conditions that discriminate between a perfect match and a single base mismatch in this hybridization of a member of said second array of nucleic acid segments to the informational portion of said second hybridization oligonucleotide,wherein said second hybridization oligonucleotide differs in sequence from said first hybridization oligonucleotide;wherein said first plurality of nucleic acid segments is not contacted with said second hybridization oligonucleotide;detecting hybridization of a hybridization oligonucleotide to a nucleic acid segment; and analyzing the result.