Patent ID: 8829172
Filing Date: 2014-09-09
Classification: C12N,C12P

Abstract:
1. A method of generating a barcoded Paired-End Ditag (bPED) nucleic acid fragment, comprising: (a) performing a first ligation by ligating a half-adaptor to a target nucleic acid to obtain a nucleic acid fragment with the half-adaptor attached to the two ends thereof, the half adaptor comprising a half-barcode and a restriction enzyme (RE) recognition site, wherein the half-adaptor is: (b) performing a second ligation by ligating the half-adaptors at the two ends of the nucleic acid fragment to form a circularized nucleic acid construct, wherein the circularized nucleic acid construct comprises a full-size barcoded adaptor; and (c) digesting the circularized nucleic acid construct with a RE that cleaves at a defined distance from the RE recognition site, and thereby generating the bPED nucleic acid fragment, wherein the bPED nucleic acid fragment comprises: