Patent ID: 6197527
Filing Date: 2001-03-06
Classification: C12N,G01N

Abstract:
A method of measuring the activity of a topoisomerase in the presence of a potential topoisomerase activity modulator, the method comprising:incubating a test mixture comprising: a topoisomerase comprising an epitope, a tagged nucleic acid comprising a tag, and a potential activity modulator;adding a denaturant to the test mixture;binding the tag to a solid support, thereby binding the tagged nucleic acid to the solid support;washing the solid support in a high salt solution;contacting the solid support-bound tagged nucleic acid with a labeling moiety comprising a primary label and a binding moiety that specifically binds to the epitope; anddetermining whether the topoisomerase is bound to the tagged nucleic acid by detecting the presence or absence of the labeling moiety on the solid support, thereby determining the activity of the topoisomerase in the test mixture.