Patent ID: 8124360
Filing Date: 2012-02-28
Classification: C07K,G01N

Abstract:
1. A method to identify an agent that modulates sweet taste signaling in taste cells comprising: (i) contacting cells that express a homomeric sweet receptor that responds to perillartine with an agent, optionally in presence of another agent; (ii) comparing receptor activity to cells that express a homomeric sweet receptor that have been contacted with perillartine but no agent; and (iii) determining whether at least one agent affects the functional activity of said homomeric sweet receptor in said cells by at least one functional response in said cells; wherein said homomeric sweet receptor comprises a polypeptide with a sequence at least 90% identical to SEQ ID NO:2. and wherein the homomeric sweet receptor expressing cells do not express a T1R3 receptor.