Patent ID: 6420112
Filing Date: 2002-07-16
Classification: C07H,C12Q,Y10S,Y10T

Abstract:
A method for attaching double-stranded DNA to a support structure, comprising:providing a mixture of a plurality of linear double stranded DNA molecules and an excess plurality of labeled dideoxy nucleotides bases; adding terminal transferase to said mixture, wherein said transferase binds said labeled dideoxy nucleotide bases to at least one 3â€² end of a double-stranded DNA molecule forming a plurality of a labeled double-stranded DNA molecules and a plurality of unbound bases; removing said unbound bases from said mixture; and adding said mixture a plurality of support structures, wherein each structure comprises a first ligand that binds to said labeled DNA, wherein upon said addition said plurality of support structures binds to said labeled double-stranded DNA molecules.