Patent ID: 8362217
Filing Date: 2013-01-29
Classification: C07K

Abstract:
1. A method for purifying a biomolecule from a mixture containing impurities comprising: a. providing the mixture at a set of pH conditions, b. adding to said mixture one or more polymers selected from the group consisting of polyvinylpyridine and copolymers of vinylpyridine, soluble in said mixture under the set of pH conditions and capable of reversibly and selectively binding to the biomolecule, c. mixing the one or more solubilized polymers throughout the mixture; d. precipitating the one or more polymers by changing the set of pH conditions in the mixture, such that the precipitated polymers bind selectively and reversibly to the biomolecule; and e. separating the precipitated polymer and bound biomolecule from the mixture and wherein the biomolecule is a monoclonal antibody.