Patent ID: 7626077
Filing Date: 2009-12-01
Classification: C12N

Abstract:
1. A method of making a glyphosate-resistant plant, comprising: (a) introducing a DNA fragment into a plurality of regenerable plant cells; (b) selecting from said plurality of plant cells at lease one glyphosate-resistant plant cell; and (c) using the selected at least one glyphosate-resistant plant cell to regenerate glyphosate-resistant plants; wherein the DNA fragment comprises a non-heterologous EPSPS 5′ regulatory sequence, a coding sequence encoding a glyphosate-resistant EPSPS, wherein the EPSPS 5′ regulatory sequence is operably linked to the coding sequence, intron and exon sequences of an EPSPS genomic sequence, and a non-heterologous EPSPS 3′ regulatory sequence, and wherein the DNA fragment further comprises a non-heterologous chloroplast transit peptide but does not contain a non-EPSPS enhancer; such that when said plant is exposed to glyphosate at commercial rates, said plant is resistant to said glyphosate.