Patent ID: 6309873
Filing Date: 2001-10-30
Classification: A61K,C07K

Abstract:
A method for producing streptokinase mutants by expression of a gene encoding said mutant streptokinase which comprises the steps of transforming a host cell with an expression vector containing a streptokinase gene skc-2 fragment consisting essentially of the nucleotide sequence (Seq. ID. No. 11):ATGAACAACA GCCAATTAGT TGTTAGCGTT GCTGGTACTG 60 TTGAGGGGAC GAATCAAGAC ATTAGTCTTA AATTTTTTGA AATTGACCTA ACATCACGAC 120 CTGCTCATGG AGGAAAGACA GAGCAAGGCT TAAGTCCAAA ATCAAAACCA TTTGCTACTG 180 ATAGTGGCGC GATGCCACAT AAACTTGAAA AAGCTGACTT ACTAAAGGCT ATTCAAGAAC 240 AATTGATCGC TAACGTCCAC AGTAACGACG ACTACTTTGA GGTCATTGAT TTTGCAAGCG 300 ATGCAACCAT TACTGATCGA AACGGCAAGG TCTACTTTGC TGACAAAGAT GGTTCGGTAA 360 CCTTGCCGAC CCAACCTGTC CAAGAATTTT TGCTAAGCGG ACATGTGCGC GTTAGACCAT 420 ATAAAGAAAA ACCAATACAA AATCAAGCGA AATCTGTTGA TGTGGAATAT ACTGTACAGT 480 TTACTCCCTT AAACCCTGAT GACGATTTCA GACCAGGTCT CAAAGATACT AAGCTATTGA 540 AAACACTAGC TATCGGTGAC ACCATCACAT CTCAAGAATT ACTAGCTCAA GCACAAAGCA 600 TTTTAAACAA AACCCACCCA GGCTATACGA TTTATGAACG TGACTCCTCA ATCGTCACTC 660 ATGACAATGA CATTTTCCGT ACGATTTTAC CAATGGATCA AGAGTTTACT TACCATGTCA 720 AAAATCGGGA ACAAGCTTAT GAGATCAATA AAAAATCTGG TCTGAATGAA GAAATAAACA 780 ACACTGACCT GATCTCTGAG AAATATTACG TCCTTAAAAA AGGGGAAAAG CCGTATGATC 840 CCTTTGATCG CAGTCACTTG AAACTGTTCA CCATCAAATA CGTTGATGTC AACACCAACG 900 AATTGCTAAA AAGCGAGCAG CTCTTAACAG CTAGCGAACG TAACTTAGAC TTCAGAGATT 960 TATACGATCC TCGTGATAAG GCTAAACTAC TCTACAACAA TCTCGATGCT TTTGGTATTA 1020 TGGACTATAC CTTAACTGGA AAAGTAGAGG ATAATCACGA TGACACCAAC CGTATCATAA 1080 CCGTTTATAT GGGCAAGCGA CCCGAAGGAG AGAATGCTAG CTATCATTTA GCCTATGATA 1140 AAGATCGTTA TACCGAAGAA GAACGAGAAG TTTACAGCTA CCTGCGTTAT ACAGGGACAC 1200 CTATACCTGA TAACCCTAAC GACAAATAA 1209wherein said streptokinase mutants have reduced antigenicity compared to a full length SKC-2 streptokinase.