Patent ID: 9120844
Filing Date: 2015-09-01
Classification: C07K,C12N,C12Y

Abstract:
1. A method for changing conformation of globular proteins, comprising steps of: preparing a globular protein; mixing the globular protein with water to form a mixture solution; allowing the mixture solution to stand for a first adsorption time without using a binding layer, so that the globular protein in the mixture solution is adsorbed onto an air/liquid interface of the mixture solution to form a protein monolayer, wherein the protein monolayer has a first surface pressure and the conformation of the protein monolayer is mainly β-sheet; and allowing the mixture solution to stand for a second adsorption time, so that the protein monolayer has a second surface pressure and the conformation of the protein monolayer is converted into α-helix; wherein the second surface pressure is higher than the first surface pressured the globular protein is glucose oxidase; and the concentration of the globular protein of the mixture solution is between 4.98 mg/L and 19.92 mg/L.