Patent ID: 7691609
Filing Date: 2010-04-06
Classification: A21D,A23K,C12N,C12Y

Abstract:
1. A method for increasing the expression efficiency of a Family 11 xylanase from a Trichoderma host comprising: (a) introducing a functional consensus N-glycosylation site into a Family 11 xylanase coding region that is not found in a parental Family 11 xylanase coding region by substitution of the native amino acid at one or more of positions 34, 131, 180 and 182 with an asparagine, the position being determined by alignment of the Family 11 xylanase with the amino acid sequence of SEQ ID NO: 1, to produce a modified Family 11 xylanase coding region; (b) incorporating a DNA encoding the modified Family 11 xylanase coding region produced in step (a) into a genetic construct comprising a promoter and a secretion signal sequence operatively linked to said modified Family 11 xylanase coding region; (c) transforming a (d) culturing the genetically modified wherein the expression efficiency of the modified Family 11 xylanase is at least 40% higher than that of a parental Family 11 xylanase when expressed using the same method.