Patent ID: 8735062
Filing Date: 2014-05-27
Classification: C12Q,G01N

Abstract:
1. A method comprising: a) providing a sample suspected of containing a nucleic acid of interest, b) contacting the sample with a nucleic acid molecule complementary to the nucleic acid of interest to form a nucleic acid duplex; wherein said nucleic acid molecule complementary to the nucleic acid of interest is covalently or non-covalently attached to a chemiluminescent molecule, wherein said chemiluminescent molecule is selected from the group consisting of blue light luciferases; c) contacting the sample with an intercalating dye to generate a dye-bound nucleic acid duplex, wherein said dye is excited in a range between 450 and 500 nM; d) activating said chemiluminescent molecule to produce light, wherein the light excites the intercalating dye in the dye-bound duplex, wherein said activating is initiated by adding a chemiluminescent substrate to the sample and whereby no light source other than the chemiluminescent molecule is used; and e) detecting the light emitted by the intercalating dye whereby a nucleic acid of interest is detected; wherein the light detected is in a range greater than 500 nM.