Patent ID: 6414123
Filing Date: 2002-07-02
Classification: C07K

Abstract:
A method for purification of follicle stimulating hormone (FSH) from an FSH-containing sample comprising the steps of:(a) applying the sample in a first buffer comprising a pH of less than about 7.5 to a dye affinity chromatography matrix comprising a dye ligand; (b) washing out contaminants from the chromatography matrix with a second buffer comprising a pH of less than about 9.0; (c) and eluting the FSH with a third buffer comprising less than about 0.8 M NaCl; wherein the FSH is selected from a group consisting of human recombinant FSH, human FSH secreted from gonadotropes maintained in cell culture, genetically altered forms of human FSH, bovine FSH, equine FSH, porcine FSH, ovine FSH, canine FSH, rat FSH, feline FSH, mouse FSH, and monkey FSH.