Patent ID: 7291494
Filing Date: 2007-11-06
Classification: A61K,C07K,C12N,C12Q,G01N

Abstract:
1. A method of identifying an agent that affects isopeptidase activity of an Rpn11 polypeptide having the amino acid sequence of SEQ ID NO:23 or 24 comprising: incubating a test agent with the Rpn11 polypeptide in the presence of a modifier protein and a target protein, wherein the Rpn11 polypeptide has isopeptidase activity and deconjugates the modifier protein from the target protein; and determining the isopeptidase activity of the polypeptide—by measuring deconjugation of the modifier protein from the target protein in the presence and absence of the test agent, wherein a difference in the isopeptidase activity in the presence versus the absence of the test agent is indicative of an agent that affects isopeptidase activity of the Rpn11 polypeptide, wherein the modifier protein is a ubiquitin or a ubiquitin-like modifier protein.