Patent ID: 7572583
Filing Date: 2009-08-11
Classification: C12Q

Abstract:
1. An oligonucleotide primer for amplifying a random subset of restriction fragments obtained from a target DNA using at least one restriction endonuclease, wherein the restriction fragments are ligated to an adaptor sequence which is a double-stranded synthetic oligonucleotide (a) one end of which is designed for ligation to one end or both ends of the restriction fragments generated with said at least one restriction endonuclease, and (b) the other end of which cannot be ligated to said restriction fragments; and wherein said oligonucleotide primer comprises a nucleotide sequence selected from the group consisting of: a primer enabling amplification of restriction fragments ligated with a PstI adaptor: a primer enabling amplification of restriction fragments ligated with a MseI adaptor: a primer enabling amplification of restriction fragments ligated with a TaqI adaptor: a primer enabling amplification of restriction fragments ligated with a EcoRI adaptor: a primer enabling amplification of restriction fragments ligated with a AseI adaptor: a primer enabling amplification of restriction fragments ligated with a Sse8387-I adaptor: a primer enabling amplification of restriction fragments ligated with a ApaI adaptor: