Patent ID: 6054047
Filing Date: 2000-04-25
Classification: B01J,C40B,G01N,H01J

Abstract:
An apparatus for screening a plurality of compound libraries to determine the relative or absolute affinity of a plurality of putative ligands in each library to a target receptor, which apparatus comprises:(a) a plurality of columns each comprising a target receptor optionally attached to a solid phase support and each having a inflow end and an outflow end, wherein each of said columns is capable of independently having a compound library comprising a plurality of putative ligands continuously applied thereto under frontal chromatography conditions whereby the target ligand is continuously contacted with the compound library to produce an effluent from the outflow end of the column;(b) a plurality of first reservoirs each connected to the inflow end of one of the columns for continuously applying a compound library to the columns;(c) a mass spectrometer connected to the outflow end of each of said columns for intermittently analyzing the effluent from each of the column.