Patent ID: 6387258
Filing Date: 2002-05-14
Classification: A61P,C07D,C12P

Abstract:
A process for isolating a statin compound from a fermentation broth, wherein the statin compound comprises a carboxylic acid capable of forming a lactone and a fused bicyclic ring, comprising the steps of:(a) pretreating the fermentation broth under alkaline conditions to remove non-polar impurities; (b) extracting the statin compound from the fermentation broth by contacting the fermentation broth with an extraction solvent, and extracting the compound from the fermentation broth into the extraction solvent, wherein the extraction solvent is a hydrophobic organic extraction solvent; (c) separating the hydrophobic organic extraction solvent from the fermentation broth; (d) concentrating a solution of the hydrophobic organic extraction solvent containing the extracted statin compound; and (e) purifying the extracted statin compound by crystallization.