Patent ID: 6342254
Filing Date: 2002-01-29
Classification: A23B,A61K,A61P,A61Q,Y10S

Abstract:
A process for producing a purified dorman extract which can inhibit proliferation of target cells or target tissue, comprising:(a) providing producer cells or producer tissue derived from a Narcissus bulb which is xenogeneic to said target cells or target tissue and which is capable of entering into a dormant state; (b) inducing said producer cells or produce tissue to enter into a dormant state by exposure to high temperature for a sufficient period of time; and (c) conducting a water extraction of the dormant producer cells or dormant producer tissue to obtain a water-soluble dorman extract, whereby the extract is fractionated so as to obtain a purified dorman extract which is capable of passing through a 0.45 uM filter from said dormant producer cells or dormant producer tissue or from a medium in which said dormant cells or tissue was incubated, the dorman extract displaying a reversible cell anti-proliferative activity without substantial cytotoxicity , wherein the activity is substantially higher in said fraction than the same fraction obtained from said producer cells or tissue when in non-dormant state.