Patent ID: 8431402
Filing Date: 2013-04-30
Classification: A01G,A01H

Abstract:
1. A method of regenerating sorghum via organogenesis comprising the steps: (a) culturing a sorghum explant on a first medium comprising MS mineral salts, N6 vitamins, 25 mg to 200 mg L-proline, 50 mg to 250 mg L-glutamine, a carbon source, 2.62 μM to 9.04 μM 2,4-dichlorophenoxy acetic acid (2,4-D) and 0.94 μM to 18.9 μM abscisic acid (ABA) to produce swelling and callusing of plumules in and around cotyledon nodal regions (SCPC), wherein the sorghum explant is a dissected embryonated cotyledon; (b) injuring the SCPC and culturing the injured SCPC on a second medium comprising MS mineral salts, B5 vitamins, 100 mg to 1000 mg L-proline, 100 mg to 1000 mg L-glutamine, 2 mg to 20 mg L-glycine, 1.42 μM to 11.36 μM ascorbic acid, 0.25 g to 1.5 g polyvinyl pyrrolidone (PVP), 0.25 g to 1.5 g polyvinyl polypyrrolidone (PVPP), 0.2 mg to 1.5 mg AgNO (c) culturing tissue with induced shoot buds on a third medium comprising MS mineral salts, B5 vitamins, 100 mg to 1000 mg L-glutamine, 5 mg to 25 mg L-glycine, 0.25 g to 1.5 g PVP, 25 mg to 200 mg adenine sulfate (AdSO (d) splitting the tissue with proliferating shoot buds into clumps of tissue with proliferating shoot buds; (e) culturing tissue with proliferating shoot buds on a fourth medium comprising MS mineral salts, B5 vitamins, 5 mg to 25 mg L-glycine, 25 mg to 200 mg AdSO (f) culturing tissue with growing shoots on a fifth medium comprising MS mineral salts, B5 vitamins, 5 mg of 25 mg L-glycine, 0.25 g to 1.5 g PVP, 25 mg to 200 mg AdSO (g) culturing tissue with growing shoots on a sixth medium comprising MS mineral salts, B5 vitamins, 5 mg to 25 mg L-glycine, 0.25 g to 1.5 g PVP, 25 mg to 200 mg AdSO