Patent ID: 6287830
Filing Date: 2001-09-11
Classification: C12N,C12P,C12R

Abstract:
deposited to Korean Culture Center of Microorganism with accession number KCCM-10160 comprising:a) fermenting glucose medium with cells of Candida magnoliae SR202 deposited to Korean Culture Center of Microorganism;b) feeding a solution containing KCl continuously or intermittently into the culture broth during erythritol production phase to maintain 2-10 (w/v) % of KCl concentration in the culture broth;c) removing the cells from the fermentation medium; andd) separating and recovering erythritol from the fermentation medium of step c),wherein the conditions for fermenting glucose medium are:i) medium composition consisting of 10-50 (w/v) % of glucose, 0.2-2.0 (w/v) % of yeast extract, 0.1-10 (w/v) % of KH.sub.2 PO.sub.4, 0.1-5.0 (w/v) % of (NH.sub.4).sub.2 SO.sub.4 and 0.01-1.0 (w/v) % of MgSO.sub.4.7H2O,ii) pH of culture medium is 6.0-8.0,iii) temperature of cultivation is 26-30.degree. C.,iv) aeration rate is 0.75-2.0 volume of air per volume of medium per minute, andv) agitation speed is 300-1