Patent ID: 9127043
Filing Date: 2015-09-08
Classification: B01D,C07K

Abstract:
1. A method of separating an antibody or a protein comprising an antibody fragment from one or more contaminants wherein said method comprises: a) contacting said antibody or protein and said one or more contaminants with a dye-ligand chromatography media; b) contacting said dye-ligand chromatography media with an organic polymer; and c) after steps a) and b), removing said antibody or said protein from contact with said dye-ligand chromatography media, wherein the molecular weight of the organic polymer is from 100 dalton to about 20,000 dalton; and wherein contacting the dye-ligand chromatography media with the organic polymer according to step b) enhances separation of the antibody or protein from the one or more contaminants as compared to separation of the antibody or protein from the one or more contaminants without contacting the dye-ligand chromatography media with the organic polymer according to step b).