Patent ID: 9057060
Filing Date: 2015-06-16
Classification: A61K,A61P,C07K,C12N,C12P,C12Y

Abstract:
1. A method of producing a mature type I pancreatic elastase protein comprising subjecting a recombinant autoactivated type I pancreatic proelastase protein to autoactivation conditions, contacting a recombinant autoactivated type I pancreatic proelastase protein with a catalytic amount of elastase, or both, said recombinant autoactivated type I pancreatic proelastase protein comprising an amino acid sequence of an elastase activation peptide sequence operably linked to an amino acid sequence of a mature type I pancreatic elastase, wherein the activation peptide is not native to the mature type I pancreatic elastase protein, and wherein: (a) the mature type I pancreatic elastase protein is a mature human type I pancreatic elastase protein and wherein the recombinant autoactivated type I pancreatic proelastase protein elastase comprises a recognition sequence of the amino acid sequence of SEQ ID NO:20; or (b) the mature type I pancreatic elastase protein is a mature porcine type I pancreatic elastase protein and the recombinant autoactivated type I pancreatic proelastase protein has a propeptide sequence comprising the amino acid sequence of SEQ ID NO:117 or SEQ ID NO:118, thereby producing a mature type I pancreatic elastase protein.