Patent ID: 6258558
Filing Date: 2001-07-10
Classification: C07B,C07K,C12N,C40B,G01N

Abstract:
A method for generating a DNA molecule which encodes a desired protein, comprising the steps of:a) providing a population of candidate RNA molecules, each of which comprises a translation initiation sequence and a start codon operably linked to a candidate protein coding sequence and each of which is covalenty bonded to a peptide acceptor at the 3' end of said candidate protein coding sequence said peptide acceptor being a molecule that can be added to the C-terminus of protein chain by the catalytic activity of a ribosomal peptidyl transferae;b) in vitro translating said candidate protein coding sequences of said candidate RNA molecules to produce a population of candidate RNA-protein fusions;c) selecting from said population a desired RNA-protein fusion based on fusion binding or activity; andd) generating from said RNA portion of said selected fusion a DNA molecule, thereby generating a DNA molecule which encodes said desired protein.