Patent ID: 6174667
Filing Date: 2001-01-16
Classification: G01N

Abstract:
A method of detecting whether a target animal is Bovine Viral Diarrhea Virus positive or negative comprising:a) providing at least one sample taken from said target animal, wherein said sample is selected from the group consisting of blood serum, mucosal fluids, milk, blood plasma, and urine;b) providing an assay system which comprises:1) a capture antibody that is a Bovine Viral Diarrhea Virus epitope specific antibody, said capture antibody being immobilized on a solid support and capable of recognizing and binding a gp48 Bovine Viral Diarrhea Virus protein or protein fragment thereof which retains antigenic specificity;2) a detector antibody that is an anti-Bovine Viral Diarrhea Virus antibody, capable of recognizing and binding said gp48 Bovine Viral Diarrhea Virus protein or protein fragment thereof which retains antigenic specificity;3) a signal generator for indicating the presence of said detector antibody operatively associated with Bovine Viral Diarrhea Virus antigen;c) analyzing the sample with said assay system to generate a change in signal if Bovine Viral Diarrhea Virus antigen is present in the sample; andd) comparing the signal to one or more reference levels to indicate whether the target animal is Bovine Viral Diarrhea Virus positive or negative.