Patent ID: 6440724
Filing Date: 2002-08-27
Classification: C12Q

Abstract:
A method for preparing samples for detecting a nucleotide sequence by means of polymerase chain reaction (PCR) where the sample is contacted with an analysis solution, comprising the steps of:a) providing a support (1) defining at least one cavity, and filling the analysis solution into said at least one cavity (2), b) placing a lid (3), which is configured complementary to a shape of the cavity (2), onto the support (1) such that at least some of the analysis solution is displaced into a gap (G) formed between the cavity (2) and the lid (3), a primer being bound to an internal face (I) of the lid (3), which extends into the cavity (2), c) sealing the gap (G) by means of at least one seal (5, 12) provided near an opening of the cavity (2) and d) shifting the nucleotide sequence toward the primer under the influence of an electrical field and binding the nucleotide sequence to the primer.