Patent ID: 9005899
Filing Date: 2015-04-14
Classification: G01N

Abstract:
1. A chitin-induced immune response based method for diagnosing allergic asthma in patients, comprising the steps of: (a) obtaining from a subject a first sample of peripheral blood immune cells and a second sample of peripheral blood immune cells; (b) mixing said first sample of peripheral blood immune cells with heparin or Na-heparin, and mixing said second sample of peripheral blood immune cells with heparin or Na-heparin; (c) adding a stimulant of chitin micro particles in an amount of 5-80 μg/ml, to the first sample; (d) performing an immunosorbent assay on the first sample and second sample; (e) measuring the average amount of IFN-γ in both said first sample and said second sample to get a first value and a second value, respectively; (f) dividing said first value with said second value to get a ratio, and (g) wherein if said ratio is smaller than a threshold value, said subject is diagnosed as an allergic asthma patient.