Patent ID: 6225636
Filing Date: 2001-05-01
Classification: G01N,G02B

Abstract:
A method of generating a labeled image from a plurality of images, referred to as dye images and associated with respective dyes, each dye image having an array of pixel values with at least one region of non-zero pixel values indicating the presence of that dye image's associated dye in that region, the method comprising:normalizing at least some pixel values within each dye image to provide a plurality of associated normalized images, each normalized image having an array of normalized pixel values spatially corresponding to the associated dye image's array of pixel values;ratioing at least some pixel values within each normalized image by dividing the spatially corresponding normalized pixel value in that normalized image by the sum of the spatially corresponding normalized pixel values in the plurality of normalized images; andlabeling a pixel in the labeled image with a given dye if and is only if the pixel value for the spatially corresponding pixel in the given dye's corresponding ratio image is above a threshold.