Patent ID: 8551547
Filing Date: 2013-10-08
Classification: A23C,A23L,A61K,A61P,C07K

Abstract:
1. A process for the preparation of an angiogenin-enriched fraction from a milk product, wherein the process uses only one cation exchange chromatography step, wherein said process comprises comprising: (a) contacting the milk product with a cation exchange resin; (b) eluting an angiogenin-depleted lactoperoxidase fraction from the cation exchange column with a mobile phase of 0.30-0.38M (1.75-2.25% w/v) sodium chloride or equivalent ionic strength; (c) eluting the angiogenin-enriched fraction from the cation exchange resin with a mobile phase; and (d) collecting the eluted angiogenin-enriched fraction, wherein the milk product is not subject to cation exchange chromatography prior to step (a) or after step (c) and wherein said angiogenin-enriched fraction comprises an angiogenin content of at least 20% w/w compared to total protein in said fraction.