Patent ID: 6331555
Filing Date: 2001-12-18
Classification: A61K

Abstract:
A method of evaluating the susceptibility of a cancer to growth inhibition by a compound comprising the steps of:(a) culturing cells of said cancer in medium containing PDGF;(b) culturing cells of said cancer in medium containing PDGF and said compound; and(c) measuring and comparing the growth of said cells in (a) and (b), wherein said compound is selected from the group consisting of:i) a compound of Formula I: ##STR11##whereinR.sub.1, R.sub.2, R'.sub.2, R"".sub.2, and R"".sub.2 are independently selected from the group consisting of hydrogen, halogen, trihalomethyl, and NO.sub.2 ; andR.sub.3 is selected from the group consisting of hydrogen, carboxy, alkoxy, and carbalkoxy;ii) a compound of Formula II: ##STR12##whereinR.sub.4 and R.sub.5 are independently selected from the group consisting of halogen, hydrogen, trihalomethyl, and NO.sub.2 ; andR.sub.6 is selected from the group consisting of aryl, alkyl, alkenyl and alkynyl;iii) a compound of Formula III: ##STR13##whereinR.sub.7, R'.sub.7, and R.sub.8 are independently selected from the group consisting of halogen, OH, hydrogen, alkoxy, SH, NH.sub.2, and C(CH.sub.3).sub.3 ; andR.sub.9 is aryl or hydrogen;iv) a compound of Formula IV: ##STR14##whereinR.sub.10 is selected from the group consisting of .dbd.S, .dbd.O, SH, OH, and NH.sub.2 ; R.sub.11 is selected from the group consisting of SH, OH, NH.sub.2, .dbd.C(CN).sub.2 and aryl; or R.sub.10 and R.sub.11 taken together are aryl; andR.sub.12 is selected from the group consisting of hydrogen, aryl, alkyl, alkenyl, and alkynyl;v) a compound of Formula V: ##STR15##wherein R.sub.13, R.sub.14, R.sub.15, R.sub.16, R.sub.17, and R.sub.18 are independently selected from the group consisting of hydrogen, halogen, alkoxy, OH, amino, alkylamino, and SH;vi) a compound of Formula VI: ##STR16##wherein R.sub.19 is selected from the group consisting of aryl, alkyl, alkenyl and alkynyl; and R.sub.20 is alkyl; or R.sub.19 and R.sub.20 are together aryl;vii) a compound of Formula VII: ##STR17##wherein b is an optional pi bond; Y and Z are independently carbon or nitrogen;R.sub.21 and R.sub.22 are independently selected from the group consisting of hydrogen, halogen, OH, SH, NH.sub.2, NO.sub.2, alkyl, alkenyl, alkynyl, alkoxy, benzoyl, COOH, and carbalkoxy; or R.sub.21 and R.sub.22 are together aryl;R.sub.23 is selected from the group consisting of hydrogen, halogen, .dbd.O, OH, SH, NH.sub.2, alkoxy, COOH, and aryl;R.sub.24 is H or aryl; andR.sub.25 is selected from the group consisting of hydrogen, halogen, .dbd.S, or .dbd.O, wherein if R.sub.25 is .dbd.O or .dbd.S then b is present as a bond;provided that if b is not a bond, the adjacent nitrogen optionally has a substituent selected from the group consisting of hydrogen, alkyl, alkyleneamino, alkyleneaminoalkly, and alkylenecyano;viii) a compound of Formula VIII: ##STR18##whereinR.sub.26 and R.sub.28 are independently selected from the group consisting of alkyl, aryl, alkenyl, and alkynyl; andR.sub.27 is aryl;ix) a compound of Formula IX: ##STR19##whereinR.sub.30 is selected from the group consisting of alkyl, alkenyl, andR.sub.31 is aryl; andR.sub.32 is either O or S;x) a compound of Formula X: ##STR20##whereinR.sub.33 is alkyl or aryl;R.sub.34, R.sub.35, and R.sub.36 are independently selected from the group consisting of halogen, OH, hydrogen, alkoxy, SH, NH.sub.2, and C(CH.sub.3).sub.3 ; andxi) a compound of any one Formula XI(a)-Formula XI(o): ##STR21## ##STR22##or a pharmaceutically acceptable salt of said compound.