Patent ID: 6964762
Filing Date: 2005-11-15
Classification: A61K,A61P,C07K,C12N,Y02A

Abstract:
1. A method of producing an immune response in a host to a pathogen, comprising the step of: administering to the host a recombinant adenovirus comprising (a) an antigen sequence that is heterologous to a native progenitor of the recombinant adenovirus and encodes a first viral antigen from a first pathogenic virus and a second viral antigen from a second pathogenic virus, said first and second viral antigens being expressed bicistronically under the transcriptional control of a first promoter located in the same region as the antigen sequence; and (b) an immuno-stimulator sequence that is heterologous to the native progenitor of the recombinant adenovirus and encodes a first immuno-stimulator expressed under the transcriptional control of a second promoter located in the same region as the immuno-stimulator sequence, wherein the antigens and immuno-stimulatory sequences are inserted into separate regions of said adenovirus, wherein said regions are selected from group consisting of E1, E3 and E4 that are partially or entirely deleted to render the recombinant adenovirus replication-incompetent, and expression of the antigen and immuno-stimulator sequences elicits an immune response directed against the first or second viral antigen upon infection of the host by the recombinant virus.