Patent ID: 7732660
Filing Date: 2010-06-08
Classification: C12N

Abstract:
1. A method for preparing a plant comprising a final chimeric DNA construct, said method comprising: preparing a final chimeric DNA construct capable of expressing a dsRNA of interest in a plant cell; said final chimeric DNA construct of interest comprising a DNA of interest (12) comprising a nucleotide sequence encoding said dsRNA of interest according to a method comprising the steps of allowing recombination to occur so as to produce a reaction mixture comprising product DNA molecules, said product DNA molecule comprising in sequence: said promoter or promoter region (3) capable of being recognized by RNA polymerases of said plant cell; a recombination site (15) which is the recombination product of said first (4) and said fifth (13) recombination site; said DNA fragment of interest (12); a recombination site (16) which is the recombination product of said second (4) and said sixth (14) recombination site; a recombination site (17) which is the recombination product of said third (5) and said sixth (14) recombination site; said DNA fragment of interest in opposite orientation (12); a recombination site (18) which is the recombination product of said fourth (7) and said fifth (13) recombination site; and said 3′ transcription terminating and polyadenylation region (8) functional in said plant cell; selecting said product DNA molecules comprising said final chimeric DNA construct; and introducing said final chimeric DNA construct into at least one cell of said plant thereby preparing a plant comprising said final chimeric DNA construct.