Patent ID: 9127249
Filing Date: 2015-09-08
Classification: A61P,C12N,C12P,C12Y

Abstract:
1. A method for preparing a 2-hydroxylated product or a 4-hydroxylated product of atorvastatin comprising: reacting at least one enzyme comprising a chimera derived from a CYP102A1 mutant with atorvastatin for a time period of at least two hours, wherein the CYP102A1 mutant has an amino acid sequence changed from that of the wild-type CYP102A1 by at least one substitution selected from the group consisting of R47L/E64G/F87V/E143G/L188Q/E267V, R47L/F81I/F87V/E143G/L188Q/E267V, and R47L/E64G/F81I/F87V/E143G/L188Q/E267V and wherein the chimera from the CYP102A1 mutant includes an amino acid substitution position and substituted amino acid in the CYP102A1 mutant selected from the group consisting of A475V/E559D/T665A/P676L/A679E/E688A/A742G/K814E/R826S/R837H/E871N/1882V/E88 8G/P895S/S955N/M968V/Q982R/A1009D/H1022Y/Q1023E, A475V/E559D/T665A/A679E/E688A/A742G/K814E/E871N/1882V/E888G/P895S/G913G/S95 5N/M968V/A1009D/H1022Y/Q1023E, and K474T/A475V/Q547E/D600EN625L/D wherein total turnover number of atorvastatin is increased as compared to same reaction in which the CYP102A1 mutant is reacted with atorvastatin.