Patent ID: 9084994
Filing Date: 2015-07-21
Classification: B01L,G01N

Abstract:
1. A multi-well filter plate for the simultaneous and parallel purification and analysis of interacting proteins by affinity chromatography, said multi-well filter plate being adapted for separation of interacting proteins from a mixture of interacting proteins, insoluble protein fragments, and protein fragments by low speed centrifugation or positive pressure and comprising a plurality of filtration wells, each filtration well consisting of an upper sample zone; a lower filtration zone; a conical flow director zone; and, an elution tip, wherein the upper sample zone and the lower filtration zone are separated by a retainer ring disposed within the lower filtration zone, the retainer permitting fluid communication between the upper sample zone and the lower filtration zone; wherein said upper sample zone comprises an upper collection zone, a sponge zone comprising a depth filter media consisting of polyester, and a deep bed filtration zone comprising a deep bed filter matrix selected from the group consisting of diatomaceous earth and silica gel; and, wherein the lower filtration zone comprises the retainer ring, a supported hydrophilic membrane comprising polyester with hydrophilic PVDF or polypropylene with hydrophilic PVDF, and-a lower bed filtration media selected from the group consisting of none or polyester, and wherein the retainer ring is an o-ring comprising polypropylene or polyethylene, and optionally disposing a second retainer ring below the lower bed filtration media to support the supported hydrophilic membrane; whereby interacting proteins collected from each filtration well without further clarification by centrifugation are directly bound with affinity beads.