Patent ID: 6432688
Filing Date: 2002-08-13
Classification: C12N,Y10S

Abstract:
A purified amino alcohol dehydrogenase having the following physicochemical properties:(a) NAD(H)-dependent; (b) a molecular weight of a part of a subunit of about 46,000 Da when determined by SDS-polyacrylamide gel electrophoresis, and of the whole molecule of about 100,000 Da when determined by gel filtration; (c) substrate specificity, such that it acts on amino alcohols, amines, and amino acids in the presence of NAD+ to produce keto alcohols, ketones, aldehydes, and keto acids, and acts on keto alcohols, ketones, aldehydes, and keto acids in the presence of NADH and ammonium ions to produce amino alcohols, amines, and amino acids; (d) thermostability, such that it is relatively stable at 30Â° C. and inactivated at 40Â° C. or higher when heated at pH 7.0 for 30 min; (e) optimum temperature of about 30Â° C. in reductive amination at pH 7.0; (f) optimum pH of 10.0 in oxidative deamination and of 7.0 in reductive animation; and (g) stability, such that its activity is stable in the presence of glycerol, serinol, or phenylmethylsulfonylfluoride.