Patent ID: 6074834
Filing Date: 2000-06-13
Classification: G01N,Y10S

Abstract:
A method of making a lipoprotein receptor-selective marker that comprises a lipoprotein particle and a stably-associated detectable moiety, said receptor-selective marker being capable of binding specifically to a lipoprotein receptor, thereby detectably labeling said receptor, said method comprising the steps of:a) providing lipoprotein particles that bind specifically to said lipoprotein receptor, said particles being suspended In an isotonic buffer comprising a salt;b) transferring said lipoprotein particles to an osmolarity regulating non-ionic labeling diluent capable of minimizing oxidation of said lipoprotein particles; andc) contacting said lipoprotein particles with a detectable moiety, thereby forming a receptor-selective marker that comprises a lipoprotein article and a stably-associated detectable moiety.