Patent ID: 7193131
Filing Date: 2007-03-20
Classification: C12N

Abstract:
1. A process for producing a multicellular dicotyledonous plant, plant organ or plant tissue having a transformed plastome, wherein the plant, plant organ or plant tissue is selected from the group consisting of potato, tomato and tobacco, the process comprising the following steps: (a) altering or disrupting the function of a gene in a plastid genome for producing a selectable or recognizable phenotype, wherein said phenotype is a pigment deficiency and/or photosynthesis deficiency; (b) separating or selecting a plant, plant organ or plant tissue having plastids expressing said phenotype from plants, plant organs or plant tissues having plastids that do not express said phenotype, thereby producing a separated or selected plant, plant organ or plant tissue having plastids expressing said phenotype; (c) transforming said plastid genome of said separated or selected plant, plant organ or plant tissue expressing said phenotype with at least one transformation vector having a restoring sequence capable of restoring said function; and (d) separating or selecting said transformed plant, plant organ or plant tissue having plastids expressing said restored function from non-transformed plant, plant organ or plant tissue which does not express said restored function.