Patent ID: 7740855
Filing Date: 2010-06-22
Classification: A61K

Abstract:
1. A method of preparing a population of autologous T-cells enriched with inactivated CD8+ cells that are reactive to CD4+ cells, said method consisting of the steps of: a) Obtaining peripheral mononuclear blood cells (PMBC) from said patient; b) Culturing said autologous cells in the presence of a nonspecific stimulating factor; c) Expanding said autologous cells in the presence of IL-2, wherein said IL-2 is provided at 5-20 U/ml; d) Culturing said expanded autologous cells in the presence of irradiated CD4-pulsed antigen presenting cells (APCs); e) Inactivating said autologous cells in the presence of an inactivating factor; f) Washing said autologous cells and resuspending the same in injection-grade saline solution; and g) Optionally freezing aliquots of between 10