Patent ID: 9163229
Filing Date: 2015-10-20
Classification: C12N,C12Q

Abstract:
1. A method of isolating cell-free nucleic acids from a sample of urine comprising: a) obtaining a cell containing urine sample from a subject and separating said sample into a cell-free fraction, containing nucleic acids in the size range of 10 to 100 base pairs, and a cell-containing fraction by filtration or centrifugation; b) selecting an anion exchange material comprising quaternary ammonium groups and which effectively adsorbs target nucleic acids or proteinous complexes thereof present within said cell-free fraction; c) applying said cell-free fraction of said urine sample containing nucleic acids or their proteinous complexes to the selected anion exchange material, and said nucleic acids or their complexes adsorb to said material to form bound target nucleic acids or their complexes; d) optionally washing said anion exchange material with an aqueous solution of a salt at which the target nucleic acids or their complexes remain bound to said anion exchange material, said washing being of sufficient volume and ionic strength to wash the non-binding or weakly binding components through said anion exchange material; e) eluting the said bound target nucleic acids or their complexes by passing through said anion exchange material an aqueous solution of increasing ionic strength to remove in succession proteins that are not bound or are weakly bound to the anion exchange material and said nucleic acids of increasing molecular weight from said material; and f) isolating the eluted nucleic acid fraction containing nucleic acids in the size range of 10 to 100 base pairs.