Patent ID: 7732136
Filing Date: 2010-06-08
Classification: B01L

Abstract:
1. A method for amplifying a nucleic acid using a device comprising at least one circular cartridge having a plurality of reaction chambers and a reservoir, said reaction chambers being connected to the reservoir via channels, at least one heating plate having at least two distinct zones that can be heated to at least two different temperatures, wherein said distinct zones are sections of a disk or sections of a ring, and a means for relative rotational displacement between said at least one circular cartridge and said at least one heating plate, allowing a cyclic variation of the temperature of the reaction chambers wherein said means for relative rotational displacement is connected to said at least one circular cartridge for displacing the cartridge in relative rotation, said method comprising: a) at least partially filling said reservoir with a fluid containing a sample of nucleic acids to be analyzed and reagents for carrying out an amplification reaction, with the exception of primers, b) distributing said fluid to the reaction chambers of said at least one circular cartridge, in which are located the primers, c) employing said means for relative rotational displacement to rotate said at least one circular cartridge to successively bring the contents of each reaction chamber to the at least two temperatures defined by the at least two distinct zones of said at least one heating plate, wherein said nucleic acid is amplified.