Patent ID: 6075181
Filing Date: 2000-06-13
Classification: A01K,C07K

Abstract:
A method for producing a transgenic mouse wherein the germ cells comprise at least one inactivated endogenous immunoglobulin heavy chain locus in which all of the J segment genes are deleted to prevent rearrangement of the locus and to prevent formation of a transcript of a rearranged locus and the expression of an endogenous immunoglobulin heavy chain from the inactivated locus, said method comprising the steps of:(a) deleting the J segment genes from at least one endogenous heavy chain locus in a mouse embryonic stem cell to prevent rearrangement of said locus and to prevent formation of a transcript of a rearranged immunoglobulin heavy chain locus, the deletion being effected by a targeting vector comprising a gene encoding a selectable marker; and(b) producing from the embryonic stem cell a transgenic mouse whose germ cells comprise at least one immunoglobulin heavy chain locus in which all of the J segment genes are deleted.