Patent ID: 6387622
Filing Date: 2002-05-14
Classification: G01N,Y10S

Abstract:
A method for a single-measurement quantification of multiple populations of cells having substantially similar binding partner densities in a flow cytometric analysis comprising the steps oflabeling a first cell population with a first ligand capable of binding to a first binding partner on the surface of said first cell population, said first ligand being directly conjugated to a first phycobihiprotein or a phycobiliprotein-containing tandem dye; labeling a second cell population with a second ligand capable of binding to a second binding partner on the surface of said second cell population, which second ligand is crosslinked to an aminodextran which is conjugated to a second phycobiliprotein or phycobilprotein-containing tandem dye; activating said first and second phycobiliproteins or phycobiliprotein-containing tandem dyes; and measuring the fluorescence intensities of each said first and second phycobiliprotein or phycobiliprotein-containing tandem dye; wherein each said first and second phycobiliprotein or phycobiliprotein-containing tandem dye upon activation produces a different detectable fluorescence intensity for each said first cell population and said second cell population.