Patent ID: 7875467
Filing Date: 2011-01-25
Classification: B82Y,C07D,G01N,Y10S

Abstract:
1. A homogenous assay method for detection or quantitation of an analyte in a sample, comprising the steps of: (a) providing a conjugate comprising a chemiluminescent acridinium sulfonamide compound attached to an analyte or analyte analog, wherein the sulfonamide of said acridinium sulfonamide compound is linked to the 9 position of the acridinium nucleus through a carbonyl group; (b) adding a predetermined amount of said conjugate to a sample containing an unknown concentration of said analyte; (c) adding an antibody specific to said analyte to form a binding complex with either said analyte or said conjugate; (d) incubating the solution of said binding complex; (e) triggering the chemiluminescence of said binding complex reaction mixture by adding peroxide in a pH range of about 6 to 10 to cause light emission without the addition of a strong acid or a strong base; (f) measuring the amount of light emission; and (g) calculating the concentration of said analyte by comparing the amount of light emitted from the reaction mixture with a standard dose response curve which relates the amount of light emitted to a known concentration of said analyte.