Patent ID: 6139734
Filing Date: 2000-10-31
Classification: G01N,H01J

Abstract:
An apparatus for effecting electrospray ionization (ESI) of liquid material eluted from a high performance liquid chromatography (HPLC) column while providing for control over speed of flow of mobile phase through said column, comprising:a conduit for elution mobile phase which is in fluid communication with a junction which splits said mobile phase into a first steam to enter said column and a second waste stream,an HPLC column in fluid communication with said junction for receiving said mobile phase and eluting from said column a moiety of interest,an ESI emitter at an end of said column downstream from said junction and in fluid communication therewith,electrodes for applying an electrical current across said waste stream,a device configured to control the speed of flow of said mobile phase through said column, whereby an elution peak over which said moiety is eluted may be broadened, as opposed to a peak obtained in the absence of said waste stream.