Patent ID: 8367316
Filing Date: 2013-02-05
Classification: C12Q,G01N

Abstract:
1. A method of detecting segment-specific activation or suppression of a Toll-like receptor (TLR) in an epithelial cell system in response to a target agent, said method comprising: providing an intestinal primary epithelial cell system for detecting gastrointestinal segment-specific activation or suppression of a Toll-like receptor (TLR) by a target agent, said system comprising an isolated human intestinal primary epithelial cell (HIPEC) line that expresses in a segment-specific manner at least one TLR selected from the group consisting of TLR1, TLR2, TLR3, TLR4, TLR5, TLR6, TLR7, TLR8, TLR9, TLR10, TLR11, TLR12, and TLR13, wherein said HIPEC line is derived from a differentiable adult human gastrointestinal stem cell (ahGISC) line derived from at least one human gastrointestinal segment selected from the group consisting of an esophagus segment, a stomach segment, a jejunum segment, an ileum segment, a duodenum segment, a cecum segment, an appendix segment, an ascending colon segment, a transverse colon segment, a descending colon segment, a sigmoid colon segment, and a rectum segment; contacting the cell system with a target agent; and subjecting the cell system to a TLR expression assay to determine a TLR expression profile of the cell system in response to the target agent, thereby determining whether the target agent activates, suppresses, or has no effect on TLR expression in the cell system.