Patent ID: 8188242
Filing Date: 2012-05-29
Classification: A61K,B01D,C07K

Abstract:
1. A method for purifying antibodies from a solution containing antibodies, the method comprising a. contacting a first solution containing antibodies to a mixed mode chromatography matrix, wherein the mixed mode chromatography matrix comprises phenylalanine, an n-alkyl acid comprising 2-10 carbons, or a t-butyl ether derivative of polymethacrylate, thereby binding at least a portion of the antibodies to the matrix; b. separating the matrix with bound antibodies from the first solution; c. eluting the bound antibodies by raising the pH of a solution in contact with the matrix and bound antibodies, thereby producing a second solution comprising eluted antibodies; d. contacting the second solution comprising eluted antibodies to a solid support matrix, the solid support matrix comprising hydroxyapatite (HT) or fluorapatite (FT), wherein at least a portion of the antibodies bind to the solid support matrix; e. separating the solid support matrix and bound antibodies from the second solution; and f. eluting the bound antibodies from the solid support matrix, thereby purifying the antibodies.