Patent ID: 8058002
Filing Date: 2011-11-15
Classification: B01J,B82Y,C07B,C07H,C12Q,C40B

Abstract:
1. A method of immobilizing and stretching a nucleic acid on a substrate comprising: a) providing a silicon substrate, b) applying a solution of ammonium fluoride or of hydrogen fluoride onto a surface of said silicon substrate, c) allowing said solution of ammonium fluoride or hydrogen fluoride to remain on said surface of said silicon substrate for a defined period of time, d) applying an aqueous solution of a nucleic acid onto said surface of said silicon substrate, e) immobilizing said nucleic acid on said substrate and then elongating said nucleic acid by a process selected from the group comprising molecular combing, spin-coating, application of a fluid flow, application of an electric field, application of convective forces in an evaporating droplet of said solution of said nucleic acid, and any combination of the aforementioned processes, wherein said method requires no chemical reagents to immobilize said nucleic acid on said substrate except for the treatment with ammonium fluoride or hydrogen fluoride, wherein, if a solution of ammonium fluoride is used, said solution of ammonium fluoride is an aqueous solution of NH wherein, if a solution of hydrogen fluoride is used, said solution of hydrogen fluoride is an aqueous solution of hydrogen fluoride, and wherein step e) occurs after step d).