Patent ID: 8951757
Filing Date: 2015-02-10
Classification: G01N

Abstract:
1. A preparation method of a fluorophore or biotin conjugated aminoacylated tRNA derived from in vitro transcribed tRNA, which comprises the following steps: 1) preparing one type of in vitro transcribed tRNA including the 3′-terminal CA sequence corresponding to a codon on DNA of an amino acid selected for target protein labeling by in vitro transcription using linearized plasmid prepared by the Fok I restriction enzyme treatment of a circular plasmid, wherein the circular plasmid is made by a method comprising: 2) performing conjugation of the in vitro transcribed tRNA prepared in step 1) and the selected amino acid by aminoacylation; and 3) preparing a fluorophore or biotin conjugated aminoacylated tRNA by conjugating fluorophore or biotin to a functional group existing on the side chain of the amino acid or α-amino group of initiator methionine constituting the aminoacylated tRNA prepared in step 2).