Patent ID: 7960104
Filing Date: 2011-06-14
Classification: C12N,C12Q

Abstract:
1. A method of identifying a sequence of a target polynucleotide, said method comprising: (a) providing a substrate comprising a plurality of locations, wherein at least 70 percent of said plurality of locations each comprises a single concatemer, and wherein each concatemer comprises multiple copies of a monomeric unit and each monomeric unit comprises: (i) a first target sequence of said target polynucleotide; (ii) a second target sequence of said target polynucleotide, wherein said first target sequence and said second target sequence are contiguous segments of said target polynucleotide; (iii) a first interspersed adaptor interposed between said first target sequence and said second target sequence, wherein said first interspersed adaptor comprises a first anchor probe hybridization site adjacent to said first target sequence and a second anchor probe hybridization site adjacent to said second target sequence, and wherein each concatemer comprises a different target polynucleotide; (b) identifying a nucleotide of said first target sequence; and (c) identifying a nucleotide of said second target sequence,