Patent ID: 6660477
Filing Date: 2003-12-09
Classification: C12Q

Abstract:
A method for determining whether an offspring of an individual afflicted with neurofibromatosis has an increased risk of developing neurofibromatosis comprising the steps of:a. amplifying one or more polymorphous DNA microsatellite markers for neurofibromatosis from a tumor of the afflicted individual; b. amplifying the one or more polymorphous DNA microsatellite markers from the blood of the afflicted individual; c. comparing the amount and length of the one or more amplified polymorphous DNA microsatellite markers from steps (a) and (b); d. establishing the loss of an allele (Loss of Heterozygosity) in the tumor of the afflicted individual, based on the comparison in step (c); e. amplifying the one or more polymorphous DNA microsatellite markers from the blood of an offspring of the afflicted individual; and f. determining which allele of the afflicted individual was inherited by the offspring, wherein inheritance of the allele that is retained in the tumor of the afflicted individual indicates an increased risk of developing neurofibromatosis.