Patent ID: 6150148
Filing Date: 2000-11-21
Classification: C12M

Abstract:
An electroporation apparatus, the apparatus comprising:receptacle means for providing a) an interface to a voltage of specified output pulsing, and b) temperature control of materials undergoing electroporation disposed within;at least two spaced apart electrodes, at least one of the electrodes contains a Peltier device;at least one opening to provide transfer of the materials;a source of energy for energizing the at least two electrodes; anda controller constructed to receive user specification of an integrated output electroporation pulsing event with a corresponding temperature profile superimposed therewith, the controller responsive to the user specification to output gating signals to the source of energy to generate the specified output pulsing and required polarity and magnitude to the Peltier device at the at least two electrodes, whereby the Peltier device and the at least two electrodes enable temperature control and electroporation.