Patent ID: 8993718
Filing Date: 2015-03-31
Classification: A61P,C07K,C12N,C12Q,G01N

Abstract:
1. An isolated molecule comprising a cleavable detectably labeled polypeptide substrate having a) an O-glycosylation site and b) a specific protease cleavage site positioned in the polypeptide such that the rate of cleavage by a specific protease at the specific protease cleavage site is different when the polypeptide is glycosylated at the O-glycosylation site than when the polypeptide is not glycosylated at the O-glycosylation site, wherein the detectable label is positioned in the polypeptide such that a change in the detectable label identifies cleavage of the polypeptide by the specific protease at the specific protease cleavage site.