Patent ID: 6316257
Filing Date: 2001-11-13
Classification: A61K,C07K,C12N

Abstract:
A method for expanding an initial T lymphocyte population in culture medium in vitro, comprising the steps of:adding an initial T lymphocyte population to a culture medium in vitro;adding to the culture medium a non-dividing mammalian cell line expressing at least one T-cell-stimulatory component selected from the group consisting of an Fc-.gamma. receptor, a cell adhesion-accessory molecule, a molecule that binds CD21, and a cytokine, wherein said cell line is not an EBV-transformed lymphoblastoid cell line (LCL);adding IL-2 to the culture medium, wherein the concentration of IL-2 is at least 10 units/ml; andincubating the culture, thereby expanding the T lymphocyte population.