Patent ID: 8703413
Filing Date: 2014-04-22
Classification: C12Q,G01N

Abstract:
1. A method for detecting fibroblast cell reprogramming in a cell culture, the method comprising: (a) contacting a fibroblast cell induced to reprogram, or its progeny, with a plurality of detectably labeled binding moieties that bind CD13 and at least one of, Rex1, DNMT3B, and/or ABCG2; and (b) detecting the binding of one or more moieties to the cell, or its progeny, to CD13 and to the at least one of Rex1, DNMT3B, and/or ABCG2 expressed by a cell in the culture; wherein downregulation of CD13 and binding to the at least one of Rex1, DNMT3B, and/or ABCG2 indicates a reprogrammed cell.