Reference: Meghan L Marre; John W McGinty; I-Ting Chow; Megan E DeNicola; Noah W Beck; Sally C Kent; Alvin C Powers; Rita Bottino; David M Harlan; Carla J Greenbaum; William W Kwok; Jon D Piganelli; Eddie A James. "Modifying Enzymes Are Elicited by ER Stress, Generating Epitopes That Are Selectively Recognized by CD4<sup>+</sup> T Cells in Patients With Type 1 Diabetes." Diabetes (2018).

Epitope: GAD 17-36
Chemical type: Peptide, no natural source
Sequence: SGDSENPGTARAWCEVAEKF
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 3900.0

Epitope: GAD 97-116
Chemical type: Peptide, no natural source
Sequence: LLPACDGERPTLAFLEDVMN
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 2300.0

Epitope: GAD 145-164
Chemical type: Peptide, no natural source
Sequence: YNWELADEPENLEEILMHCE
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 3500.0

Epitope: GAD 177-196
Chemical type: Peptide, no natural source
Sequence: RYFNELSTGLDMVGLAADWL
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 2300.0

Epitope: GAD 313-332
Chemical type: Peptide, no natural source
Sequence: SDLERRLLEAKEKGFVPFLV
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 5500.0

Epitope: lGRP 188-203
Chemical type: Peptide, no natural source
Sequence: LVAEAFEHTPGIETAS
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 14800.0

Epitope: lA2 49-66
Chemical type: Peptide, no natural source
Sequence: HLEVCLEDGLFGECEVGV
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 1600.0

Epitope: lA2 64-83
Chemical type: Peptide, no natural source
Sequence: VGVGEARPLLEVTSPVLERL
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 1500.0

Epitope: lA2 98-114
Chemical type: Peptide, no natural source
Sequence: HDDLTEYVISEEMERIP
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 2800.0

Epitope: lA2 198-216
Chemical type: Peptide, no natural source
Sequence: SLSYEPALLEPYLFHEFGS
Assay category: TCell
Result: Positive
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: lA2 198-216
Chemical type: Peptide, no natural source
Sequence: SLSYEPALLEPYLFHEFGS
Assay category: TCell
Result: Positive
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: lA2 198-216
Chemical type: Peptide, no natural source
Sequence: SLSYEPALLEPYLFHEFGS
Assay category: TCell
Result: Positive
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: lA2 198-216
Chemical type: Peptide, no natural source
Sequence: SLSYEPALLEPYLFHEFGS
Assay category: TCell
Result: Positive
Host:  33-64 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: lA2 198-216
Chemical type: Peptide, no natural source
Sequence: SLSYEPALLEPYLFHEFGS
Assay category: TCell
Result: Positive-Low
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: lA2 198-216
Chemical type: Peptide, no natural source
Sequence: SLSYEPALLEPYLFHEFGS
Assay category: TCell
Result: Positive
Host: M 27 years
Cell type: T cell CD4+
Tissue: pancreas
Culture conditions: Cell Line / Clone

Epitope: lA2 198-216
Chemical type: Peptide, no natural source
Sequence: SLSYEPALLEPYLFHEFGS
Assay category: TCell
Result: Positive
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: lA2 198-216
Chemical type: Peptide, no natural source
Sequence: SLSYEPALLEPYLFHEFGS
Assay category: TCell
Result: Positive-Low
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: lA2 198-216
Chemical type: Peptide, no natural source
Sequence: SLSYEPALLEPYLFHEFGS
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 3000.0

Epitope: lA2 319-333
Chemical type: Peptide, no natural source
Sequence: DRGEKPASPAVEPDA
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 700.0

Epitope: lA2 467-482
Chemical type: Peptide, no natural source
Sequence: AAEEYGYLVTDEKPLS
Assay category: TCell
Result: Positive
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: lA2 467-482
Chemical type: Peptide, no natural source
Sequence: AAEEYGYLVTDEKPLS
Assay category: TCell
Result: Positive
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: lA2 467-482
Chemical type: Peptide, no natural source
Sequence: AAEEYGYLVTDEKPLS
Assay category: TCell
Result: Positive
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: lA2 467-482
Chemical type: Peptide, no natural source
Sequence: AAEEYGYLVTDEKPLS
Assay category: TCell
Result: Negative
Host:  33-64 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: lA2 467-482
Chemical type: Peptide, no natural source
Sequence: AAEEYGYLVTDEKPLS
Assay category: TCell
Result: Positive-Low
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: lA2 467-482
Chemical type: Peptide, no natural source
Sequence: AAEEYGYLVTDEKPLS
Assay category: TCell
Result: Positive-Low
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: lA2 467-482
Chemical type: Peptide, no natural source
Sequence: AAEEYGYLVTDEKPLS
Assay category: TCell
Result: Positive
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: lA2 467-482
Chemical type: Peptide, no natural source
Sequence: AAEEYGYLVTDEKPLS
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 6800.0

Epitope: lA2 523-536
Chemical type: Peptide, no natural source
Sequence: QNLSLADVTEEAGL
Assay category: TCell
Result: Positive
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: lA2 523-536
Chemical type: Peptide, no natural source
Sequence: QNLSLADVTEEAGL
Assay category: TCell
Result: Positive
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: lA2 523-536
Chemical type: Peptide, no natural source
Sequence: QNLSLADVTEEAGL
Assay category: TCell
Result: Positive
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: lA2 523-536
Chemical type: Peptide, no natural source
Sequence: QNLSLADVTEEAGL
Assay category: TCell
Result: Negative
Host:  33-64 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: lA2 523-536
Chemical type: Peptide, no natural source
Sequence: QNLSLADVTEEAGL
Assay category: TCell
Result: Positive
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: lA2 523-536
Chemical type: Peptide, no natural source
Sequence: QNLSLADVTEEAGL
Assay category: TCell
Result: Positive-Low
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: lA2 523-536
Chemical type: Peptide, no natural source
Sequence: QNLSLADVTEEAGL
Assay category: TCell
Result: Positive-Low
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: lA2 523-536
Chemical type: Peptide, no natural source
Sequence: QNLSLADVTEEAGL
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 2800.0

Epitope: lA2 709-723
Chemical type: Peptide, no natural source
Sequence: LAKEWEALCAYEAEP
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 1200.0

Epitope: lA2 778-792
Chemical type: Peptide, no natural source
Sequence: PAYIATEGPLSHTIA
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 5800.0

Epitope: ChgA 4-18
Chemical type: Peptide, no natural source
Sequence: AAVLALLLCAGEVTA
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 16800.0

Epitope: ChgA 100-113
Chemical type: Peptide, no natural source
Sequence: FEDELSEVLENESS
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 3400.0

Epitope: ChgA 197-211
Chemical type: Peptide, no natural source
Sequence: PEAEGDSEGLSEGLV
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 1700.0

Epitope: ChgA 303-322
Chemical type: Peptide, no natural source
Sequence: KEEEEEMAVVPEGLFRGGKS
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 2200.0

Epitope: ChgA 325-339
Chemical type: Peptide, no natural source
Sequence: LEEEEERLSKEWEDS
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 9000.0

Epitope: Proinsulin 49-68
Chemical type: Peptide, no natural source
Sequence: FYTPKTRREAEDLEVGEVEL
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 12200.0

Epitope: Proinsulin 65-84
Chemical type: Peptide, no natural source
Sequence: EVELGGGPGAGSLEPLALEG
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 10000.0

Epitope: Proinsulin 73-92
Chemical type: Peptide, no natural source
Sequence: GAGSLEPLALEGSLEKRGIV
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 17800.0

Epitope: Proinsulin 89-108
Chemical type: Peptide, no natural source
Sequence: RGIVEECCTSICSLYELENY
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 2130.0

Epitope: GAD 89-108
Chemical type: Peptide from protein
Sequence: YAFLHATDLLPACDGERPTL
Position: 89-108
Source molecule (GenBank): NP_000809.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 3700.0

Epitope: GAD 97-116
Chemical type: Peptide from protein
Sequence: LLPACDGERPTLAFLQDVMN
Position: 97-116
Source molecule (GenBank): NP_000809.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 1200.0

Epitope: GAD 529-548
Chemical type: Peptide from protein
Sequence: VAPVIKARMMEYGTTMVSYQ
Position: 529-548
Source molecule (GenBank): NP_000809.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 1600.0

Epitope: lA2 551-565
Chemical type: Peptide from protein
Sequence: QTGVGQREEAAAVLP
Position: 551-565
Source molecule (GenBank): AAA90974.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 4200.0

Epitope: lAPP 65-84
Chemical type: Peptide from protein
Sequence: VGSNTYGKRNAVEVLKREPL
Position: 65-84
Source molecule (GenBank): CAA39504.1
Assay category: TCell
Result: Positive
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: lAPP 65-84
Chemical type: Peptide from protein
Sequence: VGSNTYGKRNAVEVLKREPL
Position: 65-84
Source molecule (GenBank): CAA39504.1
Assay category: TCell
Result: Negative
Host:  33-64 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: lAPP 65-84
Chemical type: Peptide from protein
Sequence: VGSNTYGKRNAVEVLKREPL
Position: 65-84
Source molecule (GenBank): CAA39504.1
Assay category: TCell
Result: Positive
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: lAPP 65-84
Chemical type: Peptide from protein
Sequence: VGSNTYGKRNAVEVLKREPL
Position: 65-84
Source molecule (GenBank): CAA39504.1
Assay category: TCell
Result: Positive
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: lAPP 65-84
Chemical type: Peptide from protein
Sequence: VGSNTYGKRNAVEVLKREPL
Position: 65-84
Source molecule (GenBank): CAA39504.1
Assay category: TCell
Result: Positive
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: lAPP 65-84
Chemical type: Peptide from protein
Sequence: VGSNTYGKRNAVEVLKREPL
Position: 65-84
Source molecule (GenBank): CAA39504.1
Assay category: TCell
Result: Positive-Low
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: lAPP 65-84
Chemical type: Peptide from protein
Sequence: VGSNTYGKRNAVEVLKREPL
Position: 65-84
Source molecule (GenBank): CAA39504.1
Assay category: TCell
Result: Positive-Low
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: lAPP 65-84
Chemical type: Peptide from protein
Sequence: VGSNTYGKRNAVEVLKREPL
Position: 65-84
Source molecule (GenBank): CAA39504.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 23100.0

Epitope: ChgA 81-95
Chemical type: Peptide from protein
Sequence: DLALQGAKERAHQQK
Position: 81-95
Source molecule (GenBank): P10645.7
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 18100.0

Epitope: lA2 198-216 (207E, 213Q)
Chemical type: Peptide, no natural source
Sequence: SLSYEPALLEPYLFHQFGS
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: lA2 198-216 (207Q, 213E)
Chemical type: Peptide, no natural source
Sequence: SLSYEPALLQPYLFHEFGS
Assay category: TCell
Result: Positive
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: lA2 198-216 (207Q, 213E)
Chemical type: Peptide, no natural source
Sequence: SLSYEPALLQPYLFHEFGS
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 6000.0

Epitope: lA2 523-536 (532E, 533Q)
Chemical type: Peptide, no natural source
Sequence: QNLSLADVTEQAGL
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 4200.0

Epitope: lA2 523-536 (532Q, 533E)
Chemical type: Peptide, no natural source
Sequence: QNLSLADVTQEAGL
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: lA2 545-562 (548E, 551Q, 556Q)
Chemical type: Peptide, no natural source
Sequence: TGLEILQTGVGQREEAAA
Assay category: TCell
Result: Positive
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: lA2 545-562 (548E, 551Q, 556Q)
Chemical type: Peptide, no natural source
Sequence: TGLEILQTGVGQREEAAA
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 18900.0

Epitope: lA2 545-562 (548Q, 551E, 556Q)
Chemical type: Peptide, no natural source
Sequence: TGLQILETGVGQREEAAA
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 31300.0

Epitope: lA2 545-562 (548E, 551Q, 556E)
Chemical type: Peptide, no natural source
Sequence: TGLQILQTGVGEREEAAA
Assay category: TCell
Result: Positive
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: lA2 545-562 (548E, 551Q, 556E)
Chemical type: Peptide, no natural source
Sequence: TGLQILQTGVGEREEAAA
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 18600.0

Epitope: lAPP 65-84 (73Cit, 81R)
Chemical type: Peptide from protein
Sequence: VGSNTYGKRNAVEVLKREPL
Position: 65-84
Source molecule (GenBank): CAA39504.1
Assay category: TCell
Result: Positive
Host:  12-48 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: lAPP 65-84 (73Cit, 81R)
Chemical type: Peptide from protein
Sequence: VGSNTYGKRNAVEVLKREPL
Position: 65-84
Source molecule (GenBank): CAA39504.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 31300.0

Epitope: lAPP 65-84 (73R, 81Cit)
Chemical type: Peptide from protein
Sequence: VGSNTYGKRNAVEVLKREPL
Position: 65-84
Source molecule (GenBank): CAA39504.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0