Reference: B Agrawal; M Manickasundari; E Fraga; B Singh. "T cells that recognize peptide sequences of self MHC class II molecules exist in syngeneic mice." Journal of immunology (Baltimore, Md. : 1950) (1991).

Epitope: I-Aß<sup>d</sup> 1-14
Chemical type: Peptide from protein
Sequence: GNSERHFVVQFKGE
Position: 28-41
Source molecule (GenBank): P01921.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: I-Aß<sup>d</sup> 1-14
Chemical type: Peptide from protein
Sequence: GNSERHFVVQFKGE
Position: 28-41
Source molecule (GenBank): P01921.1
Assay category: TCell
Result: Positive
Host:  8-10 weeks
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: I-Aß<sup>d</sup> 1-14
Chemical type: Peptide from protein
Sequence: GNSERHFVVQFKGE
Position: 28-41
Source molecule (GenBank): P01921.1
Assay category: TCell
Result: Positive
Host:  8-10 weeks
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: I-Aß<sup>d</sup> 9-20
Chemical type: Peptide from protein
Sequence: VQFKGECYYTNG
Position: 36-47
Source molecule (GenBank): P01921.1
Assay category: TCell
Result: Positive
Host:  8-10 weeks
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: I-Aß<sup>d</sup> 9-20
Chemical type: Peptide from protein
Sequence: VQFKGECYYTNG
Position: 36-47
Source molecule (GenBank): P01921.1
Assay category: TCell
Result: Negative
Host:  8-10 weeks
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: I-Aß<sup>d</sup> 9-20
Chemical type: Peptide from protein
Sequence: VQFKGECYYTNG
Position: 36-47
Source molecule (GenBank): P01921.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: I-Aß<sup>d</sup> 21-36
Chemical type: Peptide from protein
Sequence: TQRIRLVTRYIYNREE
Position: 48-63
Source molecule (GenBank): P01921.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: I-Aß<sup>d</sup> 21-36
Chemical type: Peptide from protein
Sequence: TQRIRLVTRYIYNREE
Position: 48-63
Source molecule (GenBank): P01921.1
Assay category: TCell
Result: Positive
Host:  8-10 weeks
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: I-Aß<sup>d</sup> 21-36
Chemical type: Peptide from protein
Sequence: TQRIRLVTRYIYNREE
Position: 48-63
Source molecule (GenBank): P01921.1
Assay category: TCell
Result: Positive
Host:  8-10 weeks
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: I-Aß<sup>d</sup> 29-46
Chemical type: Peptide from protein
Sequence: RYIYNREEYVRYDSDVGE
Position: 56-73
Source molecule (GenBank): P01921.1
Assay category: TCell
Result: Positive
Host:  8-10 weeks
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: I-Aß<sup>d</sup> 29-46
Chemical type: Peptide from protein
Sequence: RYIYNREEYVRYDSDVGE
Position: 56-73
Source molecule (GenBank): P01921.1
Assay category: TCell
Result: Positive
Host:  8-10 weeks
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: I-Aß<sup>d</sup> 29-46
Chemical type: Peptide from protein
Sequence: RYIYNREEYVRYDSDVGE
Position: 56-73
Source molecule (GenBank): P01921.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: I-Aß<sup>d</sup> 62-78
Chemical type: Peptide from protein
Sequence: NSQPEILERTRAEVDTA
Position: 89-105
Source molecule (GenBank): P01921.1
Assay category: TCell
Result: Negative
Host:  8-10 weeks
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: I-Aß<sup>d</sup> 62-78
Chemical type: Peptide from protein
Sequence: NSQPEILERTRAEVDTA
Position: 89-105
Source molecule (GenBank): P01921.1
Assay category: TCell
Result: Positive-Low
Host:  8-10 weeks
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: I-Aß<sup>d</sup> 62-78
Chemical type: Peptide from protein
Sequence: NSQPEILERTRAEVDTA
Position: 89-105
Source molecule (GenBank): P01921.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: I-Aß<sup>d</sup> 84-91
Chemical type: Peptide from protein
Sequence: EGPETSTS
Position: 111-118
Source molecule (GenBank): P01921.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: I-Aß<sup>d</sup> 84-91
Chemical type: Peptide from protein
Sequence: EGPETSTS
Position: 111-118
Source molecule (GenBank): P01921.1
Assay category: TCell
Result: Positive
Host:  8-10 weeks
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: I-Aß<sup>d</sup> 84-91
Chemical type: Peptide from protein
Sequence: EGPETSTS
Position: 111-118
Source molecule (GenBank): P01921.1
Assay category: TCell
Result: Negative
Host:  8-10 weeks
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: I-Aα<sup>d</sup> 1-17
Chemical type: Peptide from protein
Sequence: EDDIEADHVGFYGTTVY
Position: 24-40
Source molecule (GenBank): P04228.1
Assay category: TCell
Result: Positive-High
Host:  8-10 weeks
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: I-Aα<sup>d</sup> 1-17
Chemical type: Peptide from protein
Sequence: EDDIEADHVGFYGTTVY
Position: 24-40
Source molecule (GenBank): P04228.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: I-Aα<sup>d</sup> 1-17
Chemical type: Peptide from protein
Sequence: EDDIEADHVGFYGTTVY
Position: 24-40
Source molecule (GenBank): P04228.1
Assay category: TCell
Result: Positive
Host:  8-10 weeks
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: I-Aα<sup>d</sup> 26-36
Chemical type: Peptide from protein
Sequence: YTHEFDGDELF
Position: 49-59
Source molecule (GenBank): P04228.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: I-Aα<sup>d</sup> 26-36
Chemical type: Peptide from protein
Sequence: YTHEFDGDELF
Position: 49-59
Source molecule (GenBank): P04228.1
Assay category: TCell
Result: Negative
Host:  8-10 weeks
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: I-Aα<sup>d</sup> 26-36
Chemical type: Peptide from protein
Sequence: YTHEFDGDELF
Position: 49-59
Source molecule (GenBank): P04228.1
Assay category: TCell
Result: Positive
Host:  8-10 weeks
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: I-Aα<sup>d</sup> 51-69
Chemical type: Peptide from protein
Sequence: EFGQLILFEPQGGLQNIAA
Position: 74-92
Source molecule (GenBank): P04228.1
Assay category: TCell
Result: Positive-Low
Host:  8-10 weeks
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: I-Aα<sup>d</sup> 51-69
Chemical type: Peptide from protein
Sequence: EFGQLILFEPQGGLQNIAA
Position: 74-92
Source molecule (GenBank): P04228.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: I-Aα<sup>d</sup> 51-69
Chemical type: Peptide from protein
Sequence: EFGQLILFEPQGGLQNIAA
Position: 74-92
Source molecule (GenBank): P04228.1
Assay category: TCell
Result: Positive
Host:  8-10 weeks
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: I-Aα<sup>d</sup> 71-84
Chemical type: Peptide from protein
Sequence: KHNLGILTKRSNFT
Position: 94-107
Source molecule (GenBank): P04228.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: I-Aα<sup>d</sup> 71-84
Chemical type: Peptide from protein
Sequence: KHNLGILTKRSNFT
Position: 94-107
Source molecule (GenBank): P04228.1
Assay category: TCell
Result: Positive-Low
Host:  8-10 weeks
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: I-Aα<sup>d</sup> 71-84
Chemical type: Peptide from protein
Sequence: KHNLGILTKRSNFT
Position: 94-107
Source molecule (GenBank): P04228.1
Assay category: TCell
Result: Positive
Host:  8-10 weeks
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: I-Aß<sup>k</sup> 1-14
Chemical type: Peptide from protein
Sequence: GNSERHFVHQFQPF
Position: 28-41
Source molecule (GenBank): P06343.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: I-Aß<sup>k</sup> 1-14
Chemical type: Peptide from protein
Sequence: GNSERHFVHQFQPF
Position: 28-41
Source molecule (GenBank): P06343.1
Assay category: TCell
Result: Positive
Host:  8-10 weeks
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: I-Aß<sup>k</sup> 1-14
Chemical type: Peptide from protein
Sequence: GNSERHFVHQFQPF
Position: 28-41
Source molecule (GenBank): P06343.1
Assay category: TCell
Result: Positive
Host:  8-10 weeks
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo