Reference: Benedikt Agerer; Maximilian Koblischke; Venugopal Gudipati; Luis Fernando Montaño-Gutierrez; Mark Smyth; Alexandra Popa; Jakob-Wendelin Genger; Lukas Endler; David M Florian; Vanessa Mühlgrabner; Marianne Graninger; Stephan W Aberle; Anna-Maria Husa; Lisa Ellen Shaw; Alexander Lercher; Pia Gattinger; Ricard Torralba-Gombau; Doris Trapin; Thomas Penz; Daniele Barreca; Ingrid Fae; Sabine Wenda; Marianna Traugott; Gernot Walder; Winfried F Pickl; Volker Thiel; Franz Allerberger; Hannes Stockinger; Elisabeth Puchhammer-Stöckl; Wolfgang Weninger; Gottfried Fischer; Wolfgang Hoepler; Erich Pawelka; Alexander Zoufaly; Rudolf Valenta; Christoph Bock; Wolfgang Paster; René Geyeregger; Matthias Farlik; Florian Halbritter; Johannes B Huppa; Judith H Aberle; Andreas Bergthaler. "SARS-CoV-2 mutations in MHC-I-restricted epitopes evade CD8<sup>+</sup> T cell responses." Science immunology (2021).

Epitope: SARS-CoV2-S-269-277
Chemical type: Peptide from protein
Sequence: YLQPRTFLL
Position: 269-277
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host:  22-91 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: SARS-CoV2-S-269-277
Chemical type: Peptide from protein
Sequence: YLQPRTFLL
Position: 269-277
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: SARS-CoV2-S-269-277
Chemical type: Peptide from protein
Sequence: YLQPRTFLL
Position: 269-277
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host:  22-91 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: SARS-CoV2-S-269-277
Chemical type: Peptide from protein
Sequence: YLQPRTFLL
Position: 269-277
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host:  22-91 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: SARS-CoV2-S-269-277
Chemical type: Peptide from protein
Sequence: YLQPRTFLL
Position: 269-277
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Host:  22-91 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: SARS-CoV2-S-269-277
Chemical type: Peptide from protein
Sequence: YLQPRTFLL
Position: 269-277
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 59.2

Epitope: SARS-CoV2-M-89-97
Chemical type: Peptide from protein
Sequence: GLMWLSYFI
Position: 89-97
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Positive
Host:  22-91 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: SARS-CoV2-M-89-97
Chemical type: Peptide from protein
Sequence: GLMWLSYFI
Position: 89-97
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Positive
Host:  22-91 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: SARS-CoV2-M-89-97
Chemical type: Peptide from protein
Sequence: GLMWLSYFI
Position: 89-97
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Positive-Low
Host:  22-91 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: SARS-CoV2-M-89-97
Chemical type: Peptide from protein
Sequence: GLMWLSYFI
Position: 89-97
Source molecule (GenBank): P0DTC5.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 54.2

Epitope: SARS-CoV2-N-322-331
Chemical type: Peptide from protein
Sequence: MEVTPSGTWL
Position: 322-331
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  22-91 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: SARS-CoV2-N-322-331
Chemical type: Peptide from protein
Sequence: MEVTPSGTWL
Position: 322-331
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  22-91 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: SARS-CoV2-N-322-331
Chemical type: Peptide from protein
Sequence: MEVTPSGTWL
Position: 322-331
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  22-91 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: SARS-CoV2-N-322-331
Chemical type: Peptide from protein
Sequence: MEVTPSGTWL
Position: 322-331
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 55.73

Epitope: SARS-CoV2-N-322-331
Chemical type: Peptide from protein
Sequence: MEVTPSGTWL
Position: 322-331
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 30.6

Epitope: SARS-CoV2-S-821-829
Chemical type: Peptide from protein
Sequence: LLFNKVTLA
Position: 821-829
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Host:  22-91 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: SARS-CoV2-S-821-829
Chemical type: Peptide from protein
Sequence: LLFNKVTLA
Position: 821-829
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 52.6

Epitope: SARS-CoV2-S-691-699
Chemical type: Peptide from protein
Sequence: SIIAYTMSL
Position: 691-699
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host:  22-91 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: SARS-CoV2-S-691-699
Chemical type: Peptide from protein
Sequence: SIIAYTMSL
Position: 691-699
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host:  22-91 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: SARS-CoV2-S-691-699
Chemical type: Peptide from protein
Sequence: SIIAYTMSL
Position: 691-699
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 54.2

Epitope: SARS-CoV2-S-424-433
Chemical type: Peptide from protein
Sequence: KLPDDFTGCV
Position: 424-433
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Host:  22-91 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: SARS-CoV2-S-424-433
Chemical type: Peptide from protein
Sequence: KLPDDFTGCV
Position: 424-433
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 52.8

Epitope: SARS-CoV2-S-417-425
Chemical type: Peptide from protein
Sequence: KIADYNYKL
Position: 417-425
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Host:  22-91 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: SARS-CoV2-S-417-425
Chemical type: Peptide from protein
Sequence: KIADYNYKL
Position: 417-425
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 52.2

Epitope: SARS-CoV2-S-1185-1193
Chemical type: Peptide from protein
Sequence: RLNEVAKNL
Position: 1185-1193
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Host:  22-91 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: SARS-CoV2-S-1185-1193
Chemical type: Peptide from protein
Sequence: RLNEVAKNL
Position: 1185-1193
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 47.2

Epitope: SARS-CoV2-N-159-167
Chemical type: Peptide from protein
Sequence: LQLPQGTTL
Position: 159-167
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 31.6

Epitope: SARS-CoV2-N-219-227
Chemical type: Peptide from protein
Sequence: LALLLLDRL
Position: 219-227
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 22.0

Epitope: SARS-CoV2-S-mut1
Chemical type: Peptide from protein
Sequence: YFQPRTFLL
Assay category: TCell
Result: Positive-Low
Host:  22-91 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: SARS-CoV2-S-mut1
Chemical type: Peptide from protein
Sequence: YFQPRTFLL
Assay category: TCell
Result: Negative
Host:  22-91 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: SARS-CoV2-S-mut1
Chemical type: Peptide from protein
Sequence: YFQPRTFLL
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 40.4

Epitope: SARS-CoV2-M-mut1
Chemical type: Peptide from protein
Sequence: GFMWLSYFI
Position: 89-97
Source molecule (GenBank): QRV67945.1
Assay category: TCell
Result: Positive-Low
Host:  22-91 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: SARS-CoV2-M-mut1
Chemical type: Peptide from protein
Sequence: GFMWLSYFI
Position: 89-97
Source molecule (GenBank): QRV67945.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 31.4

Epitope: SARS-CoV2-N-mut1
Chemical type: Peptide from protein
Sequence: IEVTPSGTWL
Position: 322-331
Source molecule (GenBank): QRW79118.1
Assay category: TCell
Result: Negative
Host:  22-91 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: SARS-CoV2-N-mut1
Chemical type: Peptide from protein
Sequence: IEVTPSGTWL
Position: 322-331
Source molecule (GenBank): QRW79118.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 30.4

Epitope: SARS-CoV2-N-mut1
Chemical type: Peptide from protein
Sequence: IEVTPSGTWL
Position: 322-331
Source molecule (GenBank): QRW79118.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 54.53

Epitope: SARS-CoV2-S-mut2
Chemical type: Peptide from protein
Sequence: KIAYYNYKL
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 47.2

Epitope: SARS-CoV2-S-mut3
Chemical type: Peptide from protein
Sequence: KLPTDFTGCV
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 46.4

Epitope: SARS-CoV2-S-mut4
Chemical type: Peptide from protein
Sequence: KLPDEFTGCV
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 51.6

Epitope: SARS-CoV2-S-mut5
Chemical type: Peptide from protein
Sequence: LFFNKVTLA
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 30.6

Epitope: SARS-CoV2-N-mut2
Chemical type: Peptide from protein
Sequence: MEVTPSGTWF
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 30.8

Epitope: SARS-CoV2-N-mut2
Chemical type: Peptide from protein
Sequence: MEVTPSGTWF
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 52.27

Epitope: SARS-CoV2-N-mut3
Chemical type: Peptide from protein
Sequence: MEVTPSGTWS
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 31.2

Epitope: SARS-CoV2-N-mut3
Chemical type: Peptide from protein
Sequence: MEVTPSGTWS
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 46.0

Epitope: SARS-CoV2-S-mut6
Chemical type: Peptide from protein
Sequence: RLNVVAKNL
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 29.8

Epitope: SARS-CoV2-S-mut7
Chemical type: Peptide from protein
Sequence: CIIAYTMSL
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 33.4

Epitope: SARS-CoV2-S-mut9
Chemical type: Peptide from protein
Sequence: STIAYTMSL
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 47.6

Epitope: SARS-CoV2-M-mut1
Chemical type: Peptide from protein
Sequence: TLACFVLAAF
Position: 61-70
Source molecule (GenBank): QRX27467.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 32.2

Epitope: SARS-CoV2-N-mut4
Chemical type: Peptide from protein
Sequence: LVLLLLDRL
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 14.27