Reference: Davina Gorton; Brenda Govan; Colleen Olive; Natkunam Ketheesan. "B- and T-cell responses in group a streptococcus M-protein- or Peptide-induced experimental carditis." Infection and immunity (2009).

Epitope: M5-B.2
Chemical type: Peptide from protein
Sequence: ESKENEKALNELLEKTVKDK
Position: 153-172
Source molecule (GenBank): Q54510
Assay category: BCell
Result: Positive
Host: F 8-12 weeks

Epitope: M5-B.2
Chemical type: Peptide from protein
Sequence: ESKENEKALNELLEKTVKDK
Position: 153-172
Source molecule (GenBank): Q54510
Assay category: BCell
Result: Positive
Host: F 8-12 weeks

Epitope: M5-B.2
Chemical type: Peptide from protein
Sequence: ESKENEKALNELLEKTVKDK
Position: 153-172
Source molecule (GenBank): Q54510
Assay category: BCell
Result: Positive
Host: F 8-12 weeks

Epitope: M5-B.2
Chemical type: Peptide from protein
Sequence: ESKENEKALNELLEKTVKDK
Position: 153-172
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.2
Chemical type: Peptide from protein
Sequence: ESKENEKALNELLEKTVKDK
Position: 153-172
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Positive-Low
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.2
Chemical type: Peptide from protein
Sequence: ESKENEKALNELLEKTVKDK
Position: 153-172
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Negative
Host:  8-12 weeks

Epitope: M5-B.2
Chemical type: Peptide from protein
Sequence: ESKENEKALNELLEKTVKDK
Position: 153-172
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Positive-Low
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.3
Chemical type: Peptide from protein
Sequence: ELLEKTVKDKIAKEQENKET
Position: 163-182
Source molecule (GenBank): Q54510
Assay category: BCell
Result: Positive-Low
Host: F 8-12 weeks

Epitope: M5-B.3
Chemical type: Peptide from protein
Sequence: ELLEKTVKDKIAKEQENKET
Position: 163-182
Source molecule (GenBank): Q54510
Assay category: BCell
Result: Negative
Host: F 8-12 weeks

Epitope: M5-B.3
Chemical type: Peptide from protein
Sequence: ELLEKTVKDKIAKEQENKET
Position: 163-182
Source molecule (GenBank): Q54510
Assay category: BCell
Result: Positive
Host: F 8-12 weeks

Epitope: M5-B.3
Chemical type: Peptide from protein
Sequence: ELLEKTVKDKIAKEQENKET
Position: 163-182
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Positive-Low
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.3
Chemical type: Peptide from protein
Sequence: ELLEKTVKDKIAKEQENKET
Position: 163-182
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.3
Chemical type: Peptide from protein
Sequence: ELLEKTVKDKIAKEQENKET
Position: 163-182
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.3
Chemical type: Peptide from protein
Sequence: ELLEKTVKDKIAKEQENKET
Position: 163-182
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Negative
Host:  8-12 weeks

Epitope: M5-B.4
Chemical type: Peptide from protein
Sequence: IAKEQENKETIGTLKKILDE
Position: 173-192
Source molecule (GenBank): Q54510
Assay category: BCell
Result: Positive
Host: F 8-12 weeks

Epitope: M5-B.4
Chemical type: Peptide from protein
Sequence: IAKEQENKETIGTLKKILDE
Position: 173-192
Source molecule (GenBank): Q54510
Assay category: BCell
Result: Negative
Host: F 8-12 weeks

Epitope: M5-B.4
Chemical type: Peptide from protein
Sequence: IAKEQENKETIGTLKKILDE
Position: 173-192
Source molecule (GenBank): Q54510
Assay category: BCell
Result: Positive
Host: F 8-12 weeks

Epitope: M5-B.4
Chemical type: Peptide from protein
Sequence: IAKEQENKETIGTLKKILDE
Position: 173-192
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.4
Chemical type: Peptide from protein
Sequence: IAKEQENKETIGTLKKILDE
Position: 173-192
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.4
Chemical type: Peptide from protein
Sequence: IAKEQENKETIGTLKKILDE
Position: 173-192
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Positive-Low
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.4
Chemical type: Peptide from protein
Sequence: IAKEQENKETIGTLKKILDE
Position: 173-192
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Negative
Host:  8-12 weeks

Epitope: M5-B.5
Chemical type: Peptide from protein
Sequence: IGTLKKILDETVKDKIAKEQ
Position: 193-212
Source molecule (GenBank): P02977.2
Assay category: BCell
Result: Positive
Host: F 8-12 weeks

Epitope: M5-B.5
Chemical type: Peptide from protein
Sequence: IGTLKKILDETVKDKIAKEQ
Position: 193-212
Source molecule (GenBank): P02977.2
Assay category: BCell
Result: Positive-Low
Host: F 8-12 weeks

Epitope: M5-B.5
Chemical type: Peptide from protein
Sequence: IGTLKKILDETVKDKIAKEQ
Position: 193-212
Source molecule (GenBank): P02977.2
Assay category: BCell
Result: Positive
Host: F 8-12 weeks

Epitope: M5-B.5
Chemical type: Peptide from protein
Sequence: IGTLKKILDETVKDKIAKEQ
Position: 193-212
Source molecule (GenBank): P02977.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks

Epitope: M5-B.5
Chemical type: Peptide from protein
Sequence: IGTLKKILDETVKDKIAKEQ
Position: 193-212
Source molecule (GenBank): P02977.2
Assay category: TCell
Result: Positive-Low
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.5
Chemical type: Peptide from protein
Sequence: IGTLKKILDETVKDKIAKEQ
Position: 193-212
Source molecule (GenBank): P02977.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.5
Chemical type: Peptide from protein
Sequence: IGTLKKILDETVKDKIAKEQ
Position: 193-212
Source molecule (GenBank): P02977.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.6
Chemical type: Peptide from protein
Sequence: TVKDKIAKEQENKETIGTLK
Position: 168-187
Source molecule (GenBank): Q54510
Assay category: BCell
Result: Negative
Host: F 8-12 weeks

Epitope: M5-B.6
Chemical type: Peptide from protein
Sequence: TVKDKIAKEQENKETIGTLK
Position: 168-187
Source molecule (GenBank): Q54510
Assay category: BCell
Result: Positive
Host: F 8-12 weeks

Epitope: M5-B.6
Chemical type: Peptide from protein
Sequence: TVKDKIAKEQENKETIGTLK
Position: 168-187
Source molecule (GenBank): Q54510
Assay category: BCell
Result: Negative
Host: F 8-12 weeks

Epitope: M5-B.6
Chemical type: Peptide from protein
Sequence: TVKDKIAKEQENKETIGTLK
Position: 168-187
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.6
Chemical type: Peptide from protein
Sequence: TVKDKIAKEQENKETIGTLK
Position: 168-187
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Positive-Low
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.6
Chemical type: Peptide from protein
Sequence: TVKDKIAKEQENKETIGTLK
Position: 168-187
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.6
Chemical type: Peptide from protein
Sequence: TVKDKIAKEQENKETIGTLK
Position: 168-187
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Positive
Host:  8-12 weeks

Epitope: M5-B.6
Chemical type: Peptide from protein
Sequence: TVKDKIAKEQENKETIGTLK
Position: 168-187
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.6
Chemical type: Peptide from protein
Sequence: TVKDKIAKEQENKETIGTLK
Position: 168-187
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.6
Chemical type: Peptide from protein
Sequence: TVKDKIAKEQENKETIGTLK
Position: 168-187
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.6
Chemical type: Peptide from protein
Sequence: TVKDKIAKEQENKETIGTLK
Position: 168-187
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Positive-Low
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.6
Chemical type: Peptide from protein
Sequence: TVKDKIAKEQENKETIGTLK
Position: 168-187
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.6
Chemical type: Peptide from protein
Sequence: TVKDKIAKEQENKETIGTLK
Position: 168-187
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.6
Chemical type: Peptide from protein
Sequence: TVKDKIAKEQENKETIGTLK
Position: 168-187
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.7
Chemical type: Peptide from protein
Sequence: NKISEASRKGLRRDLDASRE
Position: 257-276
Source molecule (GenBank): Q54510
Assay category: BCell
Result: Positive-Low
Host: F 8-12 weeks

Epitope: M5-B.7
Chemical type: Peptide from protein
Sequence: NKISEASRKGLRRDLDASRE
Position: 257-276
Source molecule (GenBank): Q54510
Assay category: BCell
Result: Positive
Host: F 8-12 weeks

Epitope: M5-B.7
Chemical type: Peptide from protein
Sequence: NKISEASRKGLRRDLDASRE
Position: 257-276
Source molecule (GenBank): Q54510
Assay category: BCell
Result: Negative
Host: F 8-12 weeks

Epitope: M5-B.7
Chemical type: Peptide from protein
Sequence: NKISEASRKGLRRDLDASRE
Position: 257-276
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Negative
Host:  8-12 weeks

Epitope: M5-B.7
Chemical type: Peptide from protein
Sequence: NKISEASRKGLRRDLDASRE
Position: 257-276
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.7
Chemical type: Peptide from protein
Sequence: NKISEASRKGLRRDLDASRE
Position: 257-276
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.7
Chemical type: Peptide from protein
Sequence: NKISEASRKGLRRDLDASRE
Position: 257-276
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.8
Chemical type: Peptide from protein
Sequence: LRRDLDASREAKKQVEKALE
Position: 302-321
Source molecule (GenBank): Q54510
Assay category: BCell
Result: Negative
Host: F 8-12 weeks

Epitope: M5-B.8
Chemical type: Peptide from protein
Sequence: LRRDLDASREAKKQVEKALE
Position: 302-321
Source molecule (GenBank): Q54510
Assay category: BCell
Result: Negative
Host: F 8-12 weeks

Epitope: M5-B.8
Chemical type: Peptide from protein
Sequence: LRRDLDASREAKKQVEKALE
Position: 302-321
Source molecule (GenBank): Q54510
Assay category: BCell
Result: Positive-Low
Host: F 8-12 weeks

Epitope: M5-B.8
Chemical type: Peptide from protein
Sequence: LRRDLDASREAKKQVEKALE
Position: 302-321
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Negative
Host:  8-12 weeks

Epitope: M5-B.8
Chemical type: Peptide from protein
Sequence: LRRDLDASREAKKQVEKALE
Position: 302-321
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.8
Chemical type: Peptide from protein
Sequence: LRRDLDASREAKKQVEKALE
Position: 302-321
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.8
Chemical type: Peptide from protein
Sequence: LRRDLDASREAKKQVEKALE
Position: 302-321
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.9
Chemical type: Peptide from protein
Sequence: AKKQVEKALEEANSLKAALE
Assay category: BCell
Result: Positive-Low
Host: F 8-12 weeks

Epitope: M5-B.9
Chemical type: Peptide from protein
Sequence: AKKQVEKALEEANSLKAALE
Assay category: BCell
Result: Positive
Host: F 8-12 weeks

Epitope: M5-B.9
Chemical type: Peptide from protein
Sequence: AKKQVEKALEEANSLKAALE
Assay category: BCell
Result: Negative
Host: F 8-12 weeks

Epitope: M5-B.9
Chemical type: Peptide from protein
Sequence: AKKQVEKALEEANSLKAALE
Assay category: TCell
Result: Negative
Host:  8-12 weeks

Epitope: M5-B.9
Chemical type: Peptide from protein
Sequence: AKKQVEKALEEANSLKAALE
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.9
Chemical type: Peptide from protein
Sequence: AKKQVEKALEEANSLKAALE
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.9
Chemical type: Peptide from protein
Sequence: AKKQVEKALEEANSLKAALE
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.10
Chemical type: Peptide from protein
Sequence: EANSKLAALEKLNKELEESK
Position: 322-341
Source molecule (GenBank): Q54510
Assay category: BCell
Result: Negative
Host: F 8-12 weeks

Epitope: M5-B.10
Chemical type: Peptide from protein
Sequence: EANSKLAALEKLNKELEESK
Position: 322-341
Source molecule (GenBank): Q54510
Assay category: BCell
Result: Positive
Host: F 8-12 weeks

Epitope: M5-B.10
Chemical type: Peptide from protein
Sequence: EANSKLAALEKLNKELEESK
Position: 322-341
Source molecule (GenBank): Q54510
Assay category: BCell
Result: Positive
Host: F 8-12 weeks

Epitope: M5-B.10
Chemical type: Peptide from protein
Sequence: EANSKLAALEKLNKELEESK
Position: 322-341
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Negative
Host:  8-12 weeks

Epitope: M5-B.10
Chemical type: Peptide from protein
Sequence: EANSKLAALEKLNKELEESK
Position: 322-341
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.10
Chemical type: Peptide from protein
Sequence: EANSKLAALEKLNKELEESK
Position: 322-341
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M5-B.10
Chemical type: Peptide from protein
Sequence: EANSKLAALEKLNKELEESK
Position: 322-341
Source molecule (GenBank): Q54510
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo