Reference: Ana Gabriela Sitaru; Wolfgang Timmermann; Karin Ulrichs; Christoph Otto. "Hierarchical immunogenicity of donor MHC class I peptides in allotransplantation." Human immunology (2002).

Epitope: RT1.A<sup>u</sup> P1
Chemical type: Peptide from protein
Sequence: GSHSLRYFLTAVSRPGLGE
Position: 1-19
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive-High
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P1
Chemical type: Peptide from protein
Sequence: GSHSLRYFLTAVSRPGLGE
Position: 1-19
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive-High
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P1
Chemical type: Peptide from protein
Sequence: GSHSLRYFLTAVSRPGLGE
Position: 1-19
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive-High
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P1
Chemical type: Peptide from protein
Sequence: GSHSLRYFLTAVSRPGLGE
Position: 1-19
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive-High
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P1
Chemical type: Peptide from protein
Sequence: GSHSLRYFLTAVSRPGLGE
Position: 1-19
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive-High
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P1
Chemical type: Peptide from protein
Sequence: GSHSLRYFLTAVSRPGLGE
Position: 1-19
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive-High
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P1
Chemical type: Peptide from protein
Sequence: GSHSLRYFLTAVSRPGLGE
Position: 1-19
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive

Epitope: RT1.A<sup>u</sup> P2
Chemical type: Peptide from protein
Sequence: PRFIAVGYVDDTE
Position: 20-32
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P2
Chemical type: Peptide from protein
Sequence: PRFIAVGYVDDTE
Position: 20-32
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P2
Chemical type: Peptide from protein
Sequence: PRFIAVGYVDDTE
Position: 20-32
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P2
Chemical type: Peptide from protein
Sequence: PRFIAVGYVDDTE
Position: 20-32
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P2
Chemical type: Peptide from protein
Sequence: PRFIAVGYVDDTE
Position: 20-32
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P2
Chemical type: Peptide from protein
Sequence: PRFIAVGYVDDTE
Position: 20-32
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P3
Chemical type: Peptide from protein
Sequence: AENPRMEPRARWMEREGPEYW
Position: 40-60
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive

Epitope: RT1.A<sup>u</sup> P3
Chemical type: Peptide from protein
Sequence: AENPRMEPRARWMEREGPEYW
Position: 40-60
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P3
Chemical type: Peptide from protein
Sequence: AENPRMEPRARWMEREGPEYW
Position: 40-60
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P3
Chemical type: Peptide from protein
Sequence: AENPRMEPRARWMEREGPEYW
Position: 40-60
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P3
Chemical type: Peptide from protein
Sequence: AENPRMEPRARWMEREGPEYW
Position: 40-60
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P3
Chemical type: Peptide from protein
Sequence: AENPRMEPRARWMEREGPEYW
Position: 40-60
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P3
Chemical type: Peptide from protein
Sequence: AENPRMEPRARWMEREGPEYW
Position: 40-60
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P4
Chemical type: Peptide from protein
Sequence: WERETQGAKGHEQVNRVNLRTLR
Position: 60-82
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P5
Chemical type: Peptide from protein
Sequence: HTIQVMFGCDVGTDWSL
Position: 93-109
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P6
Chemical type: Peptide from protein
Sequence: RNKLERDGDADYYKAYL
Position: 144-160
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Negative
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P7
Chemical type: Peptide from protein
Sequence: GTCLESLRRYLELGKERL
Position: 162-179
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Negative

Epitope: RT1.A<sup>u</sup> P7
Chemical type: Peptide from protein
Sequence: GTCLESLRRYLELGKERL
Position: 162-179
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P7
Chemical type: Peptide from protein
Sequence: GTCLESLRRYLELGKERL
Position: 162-179
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P7
Chemical type: Peptide from protein
Sequence: GTCLESLRRYLELGKERL
Position: 162-179
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Negative
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P7
Chemical type: Peptide from protein
Sequence: GTCLESLRRYLELGKERL
Position: 162-179
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P7
Chemical type: Peptide from protein
Sequence: GTCLESLRRYLELGKERL
Position: 162-179
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>u</sup> P7
Chemical type: Peptide from protein
Sequence: GTCLESLRRYLELGKERL
Position: 162-179
Source molecule (GenBank): CAA57631.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell
Tissue: lymph node
Culture conditions: Direct Ex Vivo