Reference: Mahsa Rafieiyan; Giusto Davide Badami; Nicola Scichilone; Salvatore Corrao; Vincenzo Restivo; Enzo Massimo Farinella; Martina Bottone; Gabriella Pietra; Miriana Fallo; Andrea Maria Aglio; Marco Pio La Manna; Francesco Dieli; Mojtaba Shekarkar Azgomi; Nadia Caccamo. "HLA-E-restricted SARS-CoV-2 epitopes drive CD8<sup>+</sup> T cell memory in convalescent and vaccinated individuals: implications for the design of next-generation vaccines and immunotherapeutics." Journal of translational medicine (2026).

Epitope: Spike 320-330
Chemical type: Peptide from protein
Sequence: VQPTESIVRFP
Position: 320-330
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host:  26-92 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Spike 320-330
Chemical type: Peptide from protein
Sequence: VQPTESIVRFP
Position: 320-330
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host:  26-92 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Spike 320-330
Chemical type: Peptide from protein
Sequence: VQPTESIVRFP
Position: 320-330
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host:  26-92 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Spike 320-330
Chemical type: Peptide from protein
Sequence: VQPTESIVRFP
Position: 320-330
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host:  20-60 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Spike 320-330
Chemical type: Peptide from protein
Sequence: VQPTESIVRFP
Position: 320-330
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host:  20-60 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Spike 320-330
Chemical type: Peptide from protein
Sequence: VQPTESIVRFP
Position: 320-330
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive-High

Epitope: Spike 503-514
Chemical type: Peptide from protein
Sequence: VGYQPYRVVVLS
Position: 503-514
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host:  26-92 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Spike 503-514
Chemical type: Peptide from protein
Sequence: VGYQPYRVVVLS
Position: 503-514
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Host:  26-92 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Spike 503-514
Chemical type: Peptide from protein
Sequence: VGYQPYRVVVLS
Position: 503-514
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host:  26-92 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Spike 503-514
Chemical type: Peptide from protein
Sequence: VGYQPYRVVVLS
Position: 503-514
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive-High

Epitope: N 149-159
Chemical type: Peptide from protein
Sequence: RNPANNAAIVL
Position: 149-159
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive-Low
Host:  26-92 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: N 149-159
Chemical type: Peptide from protein
Sequence: RNPANNAAIVL
Position: 149-159
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  26-92 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: N 149-159
Chemical type: Peptide from protein
Sequence: RNPANNAAIVL
Position: 149-159
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  26-92 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: N 149-159
Chemical type: Peptide from protein
Sequence: RNPANNAAIVL
Position: 149-159
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Negative

Epitope: ORF8A 48-58
Chemical type: Peptide from protein
Sequence: RVGARKSAPLI
Position: 48-58
Source molecule (GenBank): P0DTC8.1
Assay category: TCell
Result: Positive
Host:  26-92 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF8A 48-58
Chemical type: Peptide from protein
Sequence: RVGARKSAPLI
Position: 48-58
Source molecule (GenBank): P0DTC8.1
Assay category: TCell
Result: Positive
Host:  26-92 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF8A 48-58
Chemical type: Peptide from protein
Sequence: RVGARKSAPLI
Position: 48-58
Source molecule (GenBank): P0DTC8.1
Assay category: TCell
Result: Positive
Host:  26-92 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF8A 48-58
Chemical type: Peptide from protein
Sequence: RVGARKSAPLI
Position: 48-58
Source molecule (GenBank): P0DTC8.1
Assay category: MhcBinding
Result: Positive-Low

Epitope: OFRa1b 106-116
Chemical type: Peptide from protein
Sequence: IQPGQTFSVLA
Position: 3369-3379
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Host:  26-92 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: OFRa1b 106-116
Chemical type: Peptide from protein
Sequence: IQPGQTFSVLA
Position: 3369-3379
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Host:  26-92 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: OFRa1b 106-116
Chemical type: Peptide from protein
Sequence: IQPGQTFSVLA
Position: 3369-3379
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Host:  26-92 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: OFRa1b 106-116
Chemical type: Peptide from protein
Sequence: IQPGQTFSVLA
Position: 3369-3379
Source molecule (GenBank): P0DTD1.1
Assay category: MhcBinding
Result: Positive-Intermediate

Epitope: OFRa1b 565-576
Chemical type: Peptide from protein
Sequence: TMTNRQFHQKLL
Position: 4957-4968
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Host:  26-92 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: OFRa1b 565-576
Chemical type: Peptide from protein
Sequence: TMTNRQFHQKLL
Position: 4957-4968
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Host:  26-92 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: OFRa1b 565-576
Chemical type: Peptide from protein
Sequence: TMTNRQFHQKLL
Position: 4957-4968
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Host:  26-92 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: OFRa1b 565-576
Chemical type: Peptide from protein
Sequence: TMTNRQFHQKLL
Position: 4957-4968
Source molecule (GenBank): P0DTD1.1
Assay category: MhcBinding
Result: Positive-High

Epitope: OFRa1b 17-30
Chemical type: Peptide from protein
Sequence: GLHPTQAPTHLSVD
Position: 5942-5955
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Host:  26-92 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: OFRa1b 17-30
Chemical type: Peptide from protein
Sequence: GLHPTQAPTHLSVD
Position: 5942-5955
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Host:  26-92 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: OFRa1b 17-30
Chemical type: Peptide from protein
Sequence: GLHPTQAPTHLSVD
Position: 5942-5955
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Host:  26-92 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: OFRa1b 17-30
Chemical type: Peptide from protein
Sequence: GLHPTQAPTHLSVD
Position: 5942-5955
Source molecule (GenBank): P0DTD1.1
Assay category: MhcBinding
Result: Positive-Low