Reference: Hongnuan Wang; Wei Li; Ziyue Ma; Nianzhi Zhang; Yongqiang Wang; Li Gao; Xiaoqi Li; Hong Cao; Shijun J Zheng. "Identification of novel T-cell epitopes on viral protein VP4 of Infectious Bursal Disease Virus (IBDV) that play critical roles in eliciting cellular immune response." International journal of biological macromolecules (2025).

Epitope: IBDV VP4 1-18_4/1
Chemical type: Peptide from protein
Sequence: RIAVPVVSTLFPPAAPLA
Position: 453-470
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 10-27_4/2
Chemical type: Peptide from protein
Sequence: LFPPAAPLAHAIGEGVDY
Position: 462-479
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 19-36_4/3
Chemical type: Peptide from protein
Sequence: HAIGEGVDYLLGDEAQAA
Position: 471-488
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 28-45_4/4
Chemical type: Peptide from protein
Sequence: LLGDEAQAASGTARAASG
Position: 480-497
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 37-54_4/5
Chemical type: Peptide from protein
Sequence: SGTARAASGKARAASGRI
Position: 489-506
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 46-63_4/6
Chemical type: Peptide from protein
Sequence: KARAASGRIRQLTLAADK
Position: 498-515
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 55-72_4/7
Chemical type: Peptide from protein
Sequence: RQLTLAADKGYEVVANLF
Position: 507-524
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 64-81_4/8
Chemical type: Peptide from protein
Sequence: GYEVVANLFQVPQNPVVD
Position: 516-533
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 73-90_4/9
Chemical type: Peptide from protein
Sequence: QVPQNPVVDGILASPGVL
Position: 525-542
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 91-108_4/11
Chemical type: Peptide from protein
Sequence: RGAHNLDCVLREGATLFP
Position: 543-560
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 100-117_4/12
Chemical type: Peptide from protein
Sequence: LREGATLFPVVITTVEDA
Position: 552-569
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 100-117_4/12
Chemical type: Peptide from protein
Sequence: LREGATLFPVVITTVEDA
Position: 552-569
Source molecule (GenBank): P61825
Assay category: TCell
Result: Positive
Host:  9 days
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 100-117_4/12
Chemical type: Peptide from protein
Sequence: LREGATLFPVVITTVEDA
Position: 552-569
Source molecule (GenBank): P61825
Assay category: TCell
Result: Positive-Low
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 100-117_4/12
Chemical type: Peptide from protein
Sequence: LREGATLFPVVITTVEDA
Position: 552-569
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 100-117_4/12
Chemical type: Peptide from protein
Sequence: LREGATLFPVVITTVEDA
Position: 552-569
Source molecule (GenBank): P61825
Assay category: TCell
Result: Positive-Intermediate
Host:  9 days
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 109-126_4/13
Chemical type: Peptide from protein
Sequence: VVITTVEDAMTPKALNSK
Position: 561-578
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 118-135_4/14
Chemical type: Peptide from protein
Sequence: MTPKALNSKMFAVIEGVR
Position: 570-587
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 127-144_4/15
Chemical type: Peptide from protein
Sequence: MFAVIEGVREDLQPPSQR
Position: 579-596
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 136-153_4/16
Chemical type: Peptide from protein
Sequence: EDLQPPSQRGSFIRTLSG
Position: 588-605
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 145-162_4/17
Chemical type: Peptide from protein
Sequence: GSFIRTLSGHRVYGYAPD
Position: 597-614
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 154-171_4/18
Chemical type: Peptide from protein
Sequence: HRVYGYAPDGVLPLETGR
Position: 606-623
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 163-180_4/19
Chemical type: Peptide from protein
Sequence: GVLPLETGRDYTVVPIDD
Position: 615-632
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 172-189_4/20
Chemical type: Peptide from protein
Sequence: DYTVVPIDDVWDDSIMLS
Position: 624-641
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 181-198_4/21
Chemical type: Peptide from protein
Sequence: VWDDSIMLSKDPIPPIVG
Position: 633-650
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 190-207_4/22
Chemical type: Peptide from protein
Sequence: KDPIPPIVGNSGNLAIAY
Position: 642-659
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 208-225_4/24
Chemical type: Peptide from protein
Sequence: MDVFRPKVPIHVAMTGAL
Position: 660-677
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 217-234_4/25
Chemical type: Peptide from protein
Sequence: IHVAMTGALNACGEIEKV
Position: 669-686
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 226-243_4/26
Chemical type: Peptide from protein
Sequence: NACGEIEKVSFRSTKLAT
Position: 678-695
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 235-252_4/27
Chemical type: Peptide from protein
Sequence: SFRSTKLATAHRLGLKLA
Position: 687-704
Source molecule (GenBank): P61825
Assay category: TCell
Result: Positive
Host:  9 days
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 235-252_4/27
Chemical type: Peptide from protein
Sequence: SFRSTKLATAHRLGLKLA
Position: 687-704
Source molecule (GenBank): P61825
Assay category: TCell
Result: Positive-Low
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 235-252_4/27
Chemical type: Peptide from protein
Sequence: SFRSTKLATAHRLGLKLA
Position: 687-704
Source molecule (GenBank): P61825
Assay category: TCell
Result: Positive-Intermediate
Host:  9 days
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 235-252_4/27
Chemical type: Peptide from protein
Sequence: SFRSTKLATAHRLGLKLA
Position: 687-704
Source molecule (GenBank): P61825
Assay category: TCell
Result: Positive-Intermediate
Host:  9 days
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 235-252_4/27
Chemical type: Peptide from protein
Sequence: SFRSTKLATAHRLGLKLA
Position: 687-704
Source molecule (GenBank): P61825
Assay category: TCell
Result: Positive
Host:  9 days
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 244-261_4/28
Chemical type: Peptide from protein
Sequence: AHRLGLKLAGPGAFDVNT
Position: 696-713
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IBDV VP4 253-270_4/29
Chemical type: Peptide from protein
Sequence: GPGAFDVNTGPNWATFIK
Position: 705-722
Source molecule (GenBank): P61825
Assay category: TCell
Result: Negative
Host:  9 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo