Reference: Lazar Vujanovic; Jian Shi; John M Kirkwood; Walter J Storkus; Lisa H Butterfield. "Molecular mimicry of MAGE-A6 and  HF-2 epitopes in the induction of antitumor CD8<sup>+</sup> T-cell responses." Oncoimmunology (2014).

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: MAGE-A6<sub>172-187</sub>
Chemical type: Peptide from protein
Sequence: IGHVYIFATCLGLSYD
Position: 172-187
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HF-2<sub>216-229</sub>
Chemical type: Peptide from protein
Sequence: AIYIYIFAACLLLI
Position: 216-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: HF-2<sub>216-229</sub>
Chemical type: Peptide from protein
Sequence: AIYIYIFAACLLLI
Position: 216-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: HF-2<sub>216-229</sub>
Chemical type: Peptide from protein
Sequence: AIYIYIFAACLLLI
Position: 216-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HF-2<sub>216-229</sub>
Chemical type: Peptide from protein
Sequence: AIYIYIFAACLLLI
Position: 216-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HF-2<sub>216-229</sub>
Chemical type: Peptide from protein
Sequence: AIYIYIFAACLLLI
Position: 216-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: HF-2<sub>216-229</sub>
Chemical type: Peptide from protein
Sequence: AIYIYIFAACLLLI
Position: 216-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: HF-2<sub>216-229</sub>
Chemical type: Peptide from protein
Sequence: AIYIYIFAACLLLI
Position: 216-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: HF-2<sub>216-229</sub>
Chemical type: Peptide from protein
Sequence: AIYIYIFAACLLLI
Position: 216-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HF-2<sub>216-229</sub>
Chemical type: Peptide from protein
Sequence: AIYIYIFAACLLLI
Position: 216-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HF-2<sub>216-229</sub>
Chemical type: Peptide from protein
Sequence: AIYIYIFAACLLLI
Position: 216-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: HF-2<sub>216-229</sub>
Chemical type: Peptide from protein
Sequence: AIYIYIFAACLLLI
Position: 216-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: HF-2<sub>216-229</sub>
Chemical type: Peptide from protein
Sequence: AIYIYIFAACLLLI
Position: 216-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HF-2<sub>216-229</sub>
Chemical type: Peptide from protein
Sequence: AIYIYIFAACLLLI
Position: 216-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HF-2<sub>216-229</sub>
Chemical type: Peptide from protein
Sequence: AIYIYIFAACLLLI
Position: 216-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HF-2<sub>216-229</sub>
Chemical type: Peptide from protein
Sequence: AIYIYIFAACLLLI
Position: 216-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HF-2<sub>216-229</sub>
Chemical type: Peptide from protein
Sequence: AIYIYIFAACLLLI
Position: 216-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HF-2<sub>216-229</sub>
Chemical type: Peptide from protein
Sequence: AIYIYIFAACLLLI
Position: 216-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HF-2<sub>216-229</sub>
Chemical type: Peptide from protein
Sequence: AIYIYIFAACLLLI
Position: 216-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HF-2<sub>216-229</sub>
Chemical type: Peptide from protein
Sequence: AIYIYIFAACLLLI
Position: 216-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HF-2<sub>216-229</sub>
Chemical type: Peptide from protein
Sequence: AIYIYIFAACLLLI
Position: 216-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HF-2<sub>216-229</sub>
Chemical type: Peptide from protein
Sequence: AIYIYIFAACLLLI
Position: 216-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HF-2<sub>216-229</sub>
Chemical type: Peptide from protein
Sequence: AIYIYIFAACLLLI
Position: 216-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HF-2<sub>216-229</sub>
Chemical type: Peptide from protein
Sequence: AIYIYIFAACLLLI
Position: 216-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HF-2<sub>216-229</sub>
Chemical type: Peptide from protein
Sequence: AIYIYIFAACLLLI
Position: 216-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HF-2<sub>216-229</sub>
Chemical type: Peptide from protein
Sequence: AIYIYIFAACLLLI
Position: 216-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: MAGE-A6<sub>176-185</sub>
Chemical type: Peptide from protein
Sequence: YIFATCLGLS
Position: 176-185
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: MAGE-A6<sub>176-185</sub>
Chemical type: Peptide from protein
Sequence: YIFATCLGLS
Position: 176-185
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: MAGE-A6<sub>176-185</sub>
Chemical type: Peptide from protein
Sequence: YIFATCLGLS
Position: 176-185
Source molecule (GenBank): E7EUF2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: MAGE-A6<sub>176-185</sub>
Chemical type: Peptide from protein
Sequence: YIFATCLGLS
Position: 176-185
Source molecule (GenBank): E7EUF2.1
Assay category: MhcBinding
Result: Positive-Low

Epitope: HF-2<sub>220-229</sub>
Chemical type: Peptide from protein
Sequence: YIFAACLLLI
Position: 220-229
Source molecule (GenBank): BAC44366.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HF-2<sub>220-229</sub>
Chemical type: Peptide from protein
Sequence: YIFAACLLLI
Position: 220-229
Source molecule (GenBank): BAC44366.1
Assay category: MhcBinding
Result: Positive-Low