Reference: Junbao Yang; John A Gebe; Laurie Huston; Eddie James; Venus Tan; Betty B Yue; Gerald T Nepom; William W Kwok. "H5N1 strain-specific hemagglutinin CD4+ T cell epitopes restricted by HLA DR4." Vaccine (2009).

Epitope: H5p31 (1-20)
Chemical type: Peptide from protein
Sequence: MEKIVLLFAIVSLVKSDQIC
Position: 1-20
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p32 (9-28)
Chemical type: Peptide from protein
Sequence: AIVSLVKSDQICIGYHANNS
Position: 9-28
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p33 (17-36)
Chemical type: Peptide from protein
Sequence: DQICIGYHANNSTEQVDTIM
Position: 17-36
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: H5p33 (17-36)
Chemical type: Peptide from protein
Sequence: DQICIGYHANNSTEQVDTIM
Position: 17-36
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Host:  7-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: H5p33 (17-36)
Chemical type: Peptide from protein
Sequence: DQICIGYHANNSTEQVDTIM
Position: 17-36
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p35 (33-52)
Chemical type: Peptide from protein
Sequence: DTIMEKNVTVTHAQDILEKK
Position: 33-52
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p37 (49-68)
Chemical type: Peptide from protein
Sequence: LEKKHNGKLCDLDGVKPLIL
Position: 49-68
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p38 (57-76)
Chemical type: Peptide from protein
Sequence: LCDLDGVKPLILRDCSVAGW
Position: 57-76
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: H5p38 (57-76)
Chemical type: Peptide from protein
Sequence: LCDLDGVKPLILRDCSVAGW
Position: 57-76
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: H5p38 (57-76)
Chemical type: Peptide from protein
Sequence: LCDLDGVKPLILRDCSVAGW
Position: 57-76
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Positive
Host:  7-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: H5p38 (57-76)
Chemical type: Peptide from protein
Sequence: LCDLDGVKPLILRDCSVAGW
Position: 57-76
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p39 (65-84)
Chemical type: Peptide from protein
Sequence: PLILRDCSVAGWLLGNPMCD
Position: 65-84
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p40 (73-92)
Chemical type: Peptide from protein
Sequence: VAGWLLGNPMCDEFINVPEW
Position: 73-92
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p41 (81-100)
Chemical type: Peptide from protein
Sequence: PMCDEFINVPEWSYIVEKAN
Position: 81-100
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p42 (89-108)
Chemical type: Peptide from protein
Sequence: VPEWSYIVEKANPVNDLCYP
Position: 89-108
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p43 (97-116)
Chemical type: Peptide from protein
Sequence: EKANPVNDLCYPGDFNDYEE
Position: 97-116
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p44 (105-124)
Chemical type: Peptide from protein
Sequence: LCYPGDFNDYEELKHLLSRI
Position: 105-124
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p46 (121-140)
Chemical type: Peptide from protein
Sequence: LSRINHFEKIQIIPKSSWSS
Position: 121-140
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: H5p46 (121-140)
Chemical type: Peptide from protein
Sequence: LSRINHFEKIQIIPKSSWSS
Position: 121-140
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Positive
Host:  7-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: H5p46 (121-140)
Chemical type: Peptide from protein
Sequence: LSRINHFEKIQIIPKSSWSS
Position: 121-140
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p47 (129-148)
Chemical type: Peptide from protein
Sequence: KIQIIPKSSWSSHEASLGVS
Position: 129-148
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p48 (137-156)
Chemical type: Peptide from protein
Sequence: SWSSHEASLGVSSACPYQGK
Position: 137-156
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p50 (153-172)
Chemical type: Peptide from protein
Sequence: YQGKSSFFRNVVWLIKKNST
Position: 153-172
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p52 (169-188)
Chemical type: Peptide from protein
Sequence: KNSTYPTIKRSYNNTNQEDL
Position: 169-188
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: H5p52 (169-188)
Chemical type: Peptide from protein
Sequence: KNSTYPTIKRSYNNTNQEDL
Position: 169-188
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p54 (185-204)
Chemical type: Peptide from protein
Sequence: QEDLLVLWGIHHPNDAAEQT
Position: 185-204
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p55 (193-212)
Chemical type: Peptide from protein
Sequence: GIHHPNDAAEQTKLYQNPTT
Position: 193-212
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p56 (201-220)
Chemical type: Peptide from protein
Sequence: AEQTKLYQNPTTYISVGTST
Position: 201-220
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p57 (209-228)
Chemical type: Peptide from protein
Sequence: NPTTYISVGTSTLNQRLVPR
Position: 209-228
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p58 (217-236)
Chemical type: Peptide from protein
Sequence: GTSTLNQRLVPRIATRSKVN
Position: 217-236
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p59 (225-244)
Chemical type: Peptide from protein
Sequence: LVPRIATRSKVNGQSGRMEF
Position: 225-244
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p60 (233-252)
Chemical type: Peptide from protein
Sequence: SKVNGQSGRMEFFWTILKPN
Position: 233-252
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p61 (241-260)
Chemical type: Peptide from protein
Sequence: RMEFFWTILKPNDAINFESN
Position: 241-260
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p63 (257-276)
Chemical type: Peptide from protein
Sequence: FESNGNFIAPEYAYKIVKKG
Position: 257-276
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p64 (265-284)
Chemical type: Peptide from protein
Sequence: APEYAYKIVKKGDSTIMKSE
Position: 265-284
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p65 (273-292)
Chemical type: Peptide from protein
Sequence: VKKGDSTIMKSELEYGNCNT
Position: 273-292
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p67 (289-308)
Chemical type: Peptide from protein
Sequence: NCNTKCQTPMGAINSSMPFH
Position: 289-308
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p68 (297-316)
Chemical type: Peptide from protein
Sequence: PMGAINSSMPFHNIHPLTIG
Position: 297-316
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p69 (305-324)
Chemical type: Peptide from protein
Sequence: MPFHNIHPLTIGECPKYVKS
Position: 305-324
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p70 (313-332)
Chemical type: Peptide from protein
Sequence: LTIGECPKYVKSNRLVLATG
Position: 313-332
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p71 (321-340)
Chemical type: Peptide from protein
Sequence: YVKSNRLVLATGLRNSPQRE
Position: 321-340
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p72 (329-348)
Chemical type: Peptide from protein
Sequence: LATGLRNSPQRERRRKKRGL
Position: 329-348
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p73 (337-356)
Chemical type: Peptide from protein
Sequence: PQRERRRKKRGLFGAIAGFI
Position: 337-356
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p74 (345-364)
Chemical type: Peptide from protein
Sequence: KRGLFGAIAGFIEGGWQGMV
Position: 345-364
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p75 (353-372)
Chemical type: Peptide from protein
Sequence: AGFIEGGWQGMVDGWYGYHH
Position: 353-372
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p76 (361-380)
Chemical type: Peptide from protein
Sequence: QGMVDGWYGYHHSNEQGSGY
Position: 361-380
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p77 (369-388)
Chemical type: Peptide from protein
Sequence: GYHHSNEQGSGYAADKESTQ
Position: 369-388
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p78 (377-396)
Chemical type: Peptide from protein
Sequence: GSGYAADKESTQKAIDGVTN
Position: 377-396
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: H5p78 (377-396)
Chemical type: Peptide from protein
Sequence: GSGYAADKESTQKAIDGVTN
Position: 377-396
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Positive
Host:  7-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: H5p78 (377-396)
Chemical type: Peptide from protein
Sequence: GSGYAADKESTQKAIDGVTN
Position: 377-396
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p79 (385-404)
Chemical type: Peptide from protein
Sequence: ESTQKAIDGVTNKVNSIIDK
Position: 385-404
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p80 (393-412)
Chemical type: Peptide from protein
Sequence: GVTNKVNSIIDKMNTQFEAV
Position: 393-412
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p81 (401-420)
Chemical type: Peptide from protein
Sequence: IIDKMNTQFEAVGREFNNLE
Position: 401-420
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p82 (409-428)
Chemical type: Peptide from protein
Sequence: FEAVGREFNNLERRIENLNK
Position: 409-428
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p83 (417-436)
Chemical type: Peptide from protein
Sequence: NNLERRIENLNKKMEDGFLD
Position: 417-436
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p84 (425-444)
Chemical type: Peptide from protein
Sequence: NLNKKMEDGFLDVWTYNAEL
Position: 425-444
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p86 (441-460)
Chemical type: Peptide from protein
Sequence: NAELLVLMENERTLDFHDSN
Position: 441-460
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: H5p86 (441-460)
Chemical type: Peptide from protein
Sequence: NAELLVLMENERTLDFHDSN
Position: 441-460
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Positive
Host:  7-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: H5p86 (441-460)
Chemical type: Peptide from protein
Sequence: NAELLVLMENERTLDFHDSN
Position: 441-460
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p86 (441-460)
Chemical type: Peptide from protein
Sequence: NAELLVLMENERTLDFHDSN
Position: 441-460
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: H5p87 (449-468)
Chemical type: Peptide from protein
Sequence: ENERTLDFHDSNVKNLYDKV
Position: 449-468
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p90 (473-492)
Chemical type: Peptide from protein
Sequence: RDNAKELGNGCFEFYHKCDN
Position: 473-492
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p91 (481-500)
Chemical type: Peptide from protein
Sequence: NGCFEFYHKCDNECMESVRN
Position: 481-500
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p92 (489-508)
Chemical type: Peptide from protein
Sequence: KCDNECMESVRNGTYDYPQY
Position: 489-508
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p93 (497-516)
Chemical type: Peptide from protein
Sequence: SVRNGTYDYPQYSEEARLKR
Position: 497-516
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p96 (521-540)
Chemical type: Peptide from protein
Sequence: GVKLESIGIYQILSIYSTVA
Position: 521-540
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p97 (529-548)
Chemical type: Peptide from protein
Sequence: IYQILSIYSTVASSLALAIM
Position: 529-548
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p98 (537-556)
Chemical type: Peptide from protein
Sequence: STVASSLALAIMVAGLSLWM
Position: 537-556
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p99 (545-564)
Chemical type: Peptide from protein
Sequence: LAIMVAGLSLWMCSNGSLQC
Position: 545-564
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H5p100 (553-572)
Chemical type: Peptide from protein
Sequence: SLWMCSNGSLQCRICI
Position: 553-568
Source molecule (GenBank): AAW80717.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H1HA<sub>438-457</sub>
Chemical type: Peptide from protein
Sequence: NAELLVLLENERTLDFHDSN
Position: 438-457
Source molecule (GenBank): ACA33807.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: H1HA<sub>438-457</sub>
Chemical type: Peptide from protein
Sequence: NAELLVLLENERTLDFHDSN
Position: 438-457
Source molecule (GenBank): ACA33807.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated