Reference: Satoshi Ohno; Osamu Moriya; Takayuki Yoshimoto; Hidenori Hayashi; Toshitaka Akatsuka; Masanori Matsui. "Immunogenic variation between multiple HLA-A*0201-restricted, Hepatitis C Virus-derived epitopes for cytotoxic T lymphocytes." Viral immunology (2006).

Epitope: NS5-2252
Chemical type: Peptide from protein
Sequence: ILDSFDPLV
Position: 280-288
Source molecule (GenBank): NP_751927.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: NS5-2252
Chemical type: Peptide from protein
Sequence: ILDSFDPLV
Position: 280-288
Source molecule (GenBank): NP_751927.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5-2252
Chemical type: Peptide from protein
Sequence: ILDSFDPLV
Position: 280-288
Source molecule (GenBank): NP_751927.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 714.0

Epitope: NS4-1666
Chemical type: Peptide from protein
Sequence: VLVGGVLAA
Position: 4-12
Source molecule (GenBank): NP_751925.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: NS4-1666
Chemical type: Peptide from protein
Sequence: VLVGGVLAA
Position: 4-12
Source molecule (GenBank): NP_751925.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4-1666
Chemical type: Peptide from protein
Sequence: VLVGGVLAA
Position: 4-12
Source molecule (GenBank): NP_751925.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 174.0

Epitope: Core-132
Chemical type: Peptide from protein
Sequence: DLMGYIPLV
Position: 132-140
Source molecule (GenBank): NP_751919.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Core-132
Chemical type: Peptide from protein
Sequence: DLMGYIPLV
Position: 132-140
Source molecule (GenBank): NP_751919.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: Core-132
Chemical type: Peptide from protein
Sequence: DLMGYIPLV
Position: 132-140
Source molecule (GenBank): NP_751919.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 216.0

Epitope: NS4-1769
Chemical type: Peptide from protein
Sequence: HMWNFISGI
Position: 53-61
Source molecule (GenBank): NP_751926.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4-1769
Chemical type: Peptide from protein
Sequence: HMWNFISGI
Position: 53-61
Source molecule (GenBank): NP_751926.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: NS4-1769
Chemical type: Peptide from protein
Sequence: HMWNFISGI
Position: 53-61
Source molecule (GenBank): NP_751926.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 804.0

Epitope: NS5-1992
Chemical type: Peptide from protein
Sequence: VLSDFKTWL
Position: 15-23
Source molecule (GenBank): NP_751927.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: NS5-1992
Chemical type: Peptide from protein
Sequence: VLSDFKTWL
Position: 15-23
Source molecule (GenBank): NP_751927.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5-1992
Chemical type: Peptide from protein
Sequence: VLSDFKTWL
Position: 15-23
Source molecule (GenBank): NP_751927.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 612.0

Epitope: Core-35
Chemical type: Peptide from protein
Sequence: YLLPRRGPRL
Position: 35-44
Source molecule (GenBank): NP_751919.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Core-35
Chemical type: Peptide from protein
Sequence: YLLPRRGPRL
Position: 35-44
Source molecule (GenBank): NP_751919.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: Core-35
Chemical type: Peptide from protein
Sequence: YLLPRRGPRL
Position: 35-44
Source molecule (GenBank): NP_751919.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1440.0

Epitope: NS4-1789
Chemical type: Peptide from protein
Sequence: SLMAFTAAV
Position: 78-86
Source molecule (GenBank): NP_751926.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4-1789
Chemical type: Peptide from protein
Sequence: SLMAFTAAV
Position: 78-86
Source molecule (GenBank): NP_751926.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: NS4-1789
Chemical type: Peptide from protein
Sequence: SLMAFTAAV
Position: 78-86
Source molecule (GenBank): NP_751926.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1440.0

Epitope: NS3-1585
Chemical type: Peptide from protein
Sequence: YLVAYQATV
Position: 559-567
Source molecule (GenBank): NP_803144.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: NS3-1585
Chemical type: Peptide from protein
Sequence: YLVAYQATV
Position: 559-567
Source molecule (GenBank): NP_803144.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3-1585
Chemical type: Peptide from protein
Sequence: YLVAYQATV
Position: 559-567
Source molecule (GenBank): NP_803144.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1440.0

Epitope: NS5-2145
Chemical type: Peptide from protein
Sequence: LLREEVSFRV
Position: 168-177
Source molecule (GenBank): NP_751927.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: NS5-2145
Chemical type: Peptide from protein
Sequence: LLREEVSFRV
Position: 168-177
Source molecule (GenBank): NP_751927.1
Assay category: TCell
Result: Negative
Quantitative measurement: 0.05
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5-2145
Chemical type: Peptide from protein
Sequence: LLREEVSFRV
Position: 168-177
Source molecule (GenBank): NP_751927.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 150.0

Epitope: NS4-1920
Chemical type: Peptide from protein
Sequence: WMNRLIAFA
Position: 204-212
Source molecule (GenBank): NP_751926.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: NS4-1920
Chemical type: Peptide from protein
Sequence: WMNRLIAFA
Position: 204-212
Source molecule (GenBank): NP_751926.1
Assay category: TCell
Result: Negative
Quantitative measurement: 0.07
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4-1920
Chemical type: Peptide from protein
Sequence: WMNRLIAFA
Position: 204-212
Source molecule (GenBank): NP_751926.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 162.0

Epitope: NS3-1169
Chemical type: Peptide from protein
Sequence: LLCPAGHAV
Position: 143-151
Source molecule (GenBank): NP_803144.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: NS3-1169
Chemical type: Peptide from protein
Sequence: LLCPAGHAV
Position: 143-151
Source molecule (GenBank): NP_803144.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3-1169
Chemical type: Peptide from protein
Sequence: LLCPAGHAV
Position: 143-151
Source molecule (GenBank): NP_803144.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 582.0

Epitope: NS3-1131
Chemical type: Peptide from protein
Sequence: YLVTRHADV
Position: 105-113
Source molecule (GenBank): NP_803144.1
Assay category: TCell
Result: Negative
Quantitative measurement: 0.05
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3-1131
Chemical type: Peptide from protein
Sequence: YLVTRHADV
Position: 105-113
Source molecule (GenBank): NP_803144.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: NS3-1131
Chemical type: Peptide from protein
Sequence: YLVTRHADV
Position: 105-113
Source molecule (GenBank): NP_803144.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 714.0

Epitope: NS3-1073
Chemical type: Peptide from protein
Sequence: CINGVCWTV
Position: 47-55
Source molecule (GenBank): NP_803144.1
Assay category: TCell
Result: Positive
Quantitative measurement: 0.11
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3-1073
Chemical type: Peptide from protein
Sequence: CINGVCWTV
Position: 47-55
Source molecule (GenBank): NP_803144.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: NS3-1073
Chemical type: Peptide from protein
Sequence: CINGVCWTV
Position: 47-55
Source molecule (GenBank): NP_803144.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 486.0

Epitope: NS4-1851
Chemical type: Peptide from protein
Sequence: ILAGYGAGV
Position: 140-148
Source molecule (GenBank): NP_751926.1
Assay category: TCell
Result: Positive
MHC allele types: 143
Host:  8-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: NS4-1851
Chemical type: Peptide from protein
Sequence: ILAGYGAGV
Position: 140-148
Source molecule (GenBank): NP_751926.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4-1851
Chemical type: Peptide from protein
Sequence: ILAGYGAGV
Position: 140-148
Source molecule (GenBank): NP_751926.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 288.0

Epitope: NS3-1406
Chemical type: Peptide from protein
Sequence: KLVALGINAV
Position: 380-389
Source molecule (GenBank): NP_803144.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: NS3-1406
Chemical type: Peptide from protein
Sequence: KLVALGINAV
Position: 380-389
Source molecule (GenBank): NP_803144.1
Assay category: TCell
Result: Negative
Quantitative measurement: 0.02
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3-1406
Chemical type: Peptide from protein
Sequence: KLVALGINAV
Position: 380-389
Source molecule (GenBank): NP_803144.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1440.0

Epitope: NS4-1671
Chemical type: Peptide from protein
Sequence: VLAALAAYCL
Position: 640-649
Source molecule (GenBank): CAM56207.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: NS4-1671
Chemical type: Peptide from protein
Sequence: VLAALAAYCL
Position: 640-649
Source molecule (GenBank): CAM56207.1
Assay category: TCell
Result: Positive
Quantitative measurement: 0.17
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4-1671
Chemical type: Peptide from protein
Sequence: VLAALAAYCL
Position: 640-649
Source molecule (GenBank): CAM56207.1
Assay category: MhcBinding
Result: Positive-Intermediate

Epitope: E2-686
Chemical type: Peptide from protein
Sequence: ALSTGLIHL
Position: 301-309
Source molecule (GenBank): NP_751921.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: E2-686
Chemical type: Peptide from protein
Sequence: ALSTGLIHL
Position: 301-309
Source molecule (GenBank): NP_751921.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E2-686
Chemical type: Peptide from protein
Sequence: ALSTGLIHL
Position: 301-309
Source molecule (GenBank): NP_751921.1
Assay category: MhcBinding
Result: Positive-Intermediate

Epitope: E1-220
Chemical type: Peptide from protein
Sequence: ILHTPGCV
Position: 29-36
Source molecule (GenBank): NP_751920.1
Assay category: TCell
Result: Negative
Quantitative measurement: 0.04
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E1-220
Chemical type: Peptide from protein
Sequence: ILHTPGCV
Position: 29-36
Source molecule (GenBank): NP_751920.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: E1-220
Chemical type: Peptide from protein
Sequence: ILHTPGCV
Position: 29-36
Source molecule (GenBank): NP_751920.1
Assay category: MhcBinding
Result: Positive-Intermediate

Epitope: NS4-1807
Chemical type: Peptide from protein
Sequence: LLFNILGGWV
Position: 96-105
Source molecule (GenBank): NP_751926.1
Assay category: TCell
Result: Negative
Quantitative measurement: 0.08
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4-1807
Chemical type: Peptide from protein
Sequence: LLFNILGGWV
Position: 96-105
Source molecule (GenBank): NP_751926.1
Assay category: TCell
Result: Positive
Quantitative measurement: 63.6
MHC allele types: 143
Host:  8-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: NS4-1807
Chemical type: Peptide from protein
Sequence: LLFNILGGWV
Position: 96-105
Source molecule (GenBank): NP_751926.1
Assay category: MhcBinding
Result: Positive-Intermediate

Epitope: E2-728
Chemical type: Peptide from protein
Sequence: FLLLADARAV
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E2-728
Chemical type: Peptide from protein
Sequence: FLLLADARAV
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: E2-728
Chemical type: Peptide from protein
Sequence: FLLLADARAV
Assay category: MhcBinding
Result: Positive-Low

Epitope: E2-726
Chemical type: Peptide from protein
Sequence: LLFLLLADA
Position: 338-346
Source molecule (GenBank): NP_751921.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E2-726
Chemical type: Peptide from protein
Sequence: LLFLLLADA
Position: 338-346
Source molecule (GenBank): NP_751921.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: E2-726
Chemical type: Peptide from protein
Sequence: LLFLLLADA
Position: 338-346
Source molecule (GenBank): NP_751921.1
Assay category: MhcBinding
Result: Positive-Low