Reference: Ivan Díaz; Joan Pujols; Llilianne Ganges; Mariona Gimeno; Laila Darwich; Mariano Domingo; Enric Mateu. "In silico prediction and ex vivo evaluation of potential T-cell epitopes in glycoproteins 4 and 5 and nucleocapsid protein of genotype-I (European) of porcine reproductive and respiratory syndrome virus." Vaccine (2009).

Epitope: GP4-4
Chemical type: Peptide from protein
Sequence: SYLYNADLL
Position: 93-101
Source molecule (GenBank): Q04568.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GP4-5
Chemical type: Peptide from protein
Sequence: CLFAILLAI
Position: 175-183
Source molecule (GenBank): Q04568.1
Assay category: TCell
Result: Positive
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GP4-5
Chemical type: Peptide from protein
Sequence: CLFAILLAI
Position: 175-183
Source molecule (GenBank): Q04568.1
Assay category: TCell
Result: Positive
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GP5-3
Chemical type: Peptide from protein
Sequence: CALAALVCFVIR
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GP5-3
Chemical type: Peptide from protein
Sequence: CALAALVCFVIR
Assay category: TCell
Result: Positive
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GP5-2
Chemical type: Peptide from protein
Sequence: CAFAAFVCFVIR
Position: 117-128
Source molecule (GenBank): AAA46278.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GP5-2
Chemical type: Peptide from protein
Sequence: CAFAAFVCFVIR
Position: 117-128
Source molecule (GenBank): AAA46278.1
Assay category: TCell
Result: Positive
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: N-15
Chemical type: Peptide from protein
Sequence: KPEKPHFPL
Position: 50-58
Source molecule (GenBank): Q04558.1
Assay category: TCell
Result: Positive
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: N-15
Chemical type: Peptide from protein
Sequence: KPEKPHFPL
Position: 50-58
Source molecule (GenBank): Q04558.1
Assay category: TCell
Result: Positive
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: N-6
Chemical type: Peptide from protein
Sequence: FMLPVAHTV
Position: 105-113
Source molecule (GenBank): Q04558.1
Assay category: TCell
Result: Positive
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: N-6
Chemical type: Peptide from protein
Sequence: FMLPVAHTV
Position: 105-113
Source molecule (GenBank): Q04558.1
Assay category: TCell
Result: Positive
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GP4-9
Chemical type: Peptide from protein
Sequence: FLLAGAQHI
Assay category: TCell
Result: Positive
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GP4-9
Chemical type: Peptide from protein
Sequence: FLLAGAQHI
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GP4-10
Chemical type: Peptide from protein
Sequence: FRPHGVSAA
Position: 53-61
Source molecule (GenBank): Q04568.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GP4-11
Chemical type: Peptide from protein
Sequence: MRWATTIAC
Position: 167-175
Source molecule (GenBank): Q04568.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GP5-14
Chemical type: Peptide from protein
Sequence: LFLLPTGLS
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GP5-12
Chemical type: Peptide from protein
Sequence: YQYIYNLTI
Position: 41-49
Source molecule (GenBank): AAA46278.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GP5-13
Chemical type: Peptide from protein
Sequence: FVIRAAKNC
Position: 125-133
Source molecule (GenBank): AAA46278.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: N-7
Chemical type: Peptide from protein
Sequence: IRHHLTQTE
Position: 64-72
Source molecule (GenBank): Q04558.1
Assay category: TCell
Result: Positive
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: N-7
Chemical type: Peptide from protein
Sequence: IRHHLTQTE
Position: 64-72
Source molecule (GenBank): Q04558.1
Assay category: TCell
Result: Positive
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: N-8
Chemical type: Peptide from protein
Sequence: VRLIRVTST
Position: 113-121
Source molecule (GenBank): Q04558.1
Assay category: TCell
Result: Positive
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: N-8
Chemical type: Peptide from protein
Sequence: VRLIRVTST
Position: 113-121
Source molecule (GenBank): Q04558.1
Assay category: TCell
Result: Positive
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GP5-EU-1
Chemical type: Peptide from protein
Sequence: FAAFVCFVIRAAKNC
Position: 119-133
Source molecule (GenBank): AAA46278.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GP5-EU-1
Chemical type: Peptide from protein
Sequence: FAAFVCFVIRAAKNC
Position: 119-133
Source molecule (GenBank): AAA46278.1
Assay category: TCell
Result: Positive
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GP5-EU-1V
Chemical type: Peptide from protein
Sequence: LAALVCFVIRAAKNC
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GP5-EU-1V
Chemical type: Peptide from protein
Sequence: LAALVCFVIRAAKNC
Assay category: TCell
Result: Positive
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GP5-US-1
Chemical type: Peptide from protein
Sequence: LAALICFVIRLAKNC
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GP5-US-1
Chemical type: Peptide from protein
Sequence: LAALICFVIRLAKNC
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GP5-US-2
Chemical type: Peptide from protein
Sequence: KGRLYRWRSPVIVEK
Assay category: TCell
Result: Positive
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GP5-US-2
Chemical type: Peptide from protein
Sequence: KGRLYRWRSPVIVEK
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo