Reference: Nicolas Bruffaerts; Lasse E Pedersen; Gaëlle Vandermeulen; Véronique Préat; Norbert Stockhofe-Zurwieden; Kris Huygen; Marta Romano. "Increased B and T Cell Responses in M. bovis Bacille Calmette-Guérin Vaccinated Pigs Co-Immunized with Plasmid DNA Encoding a Prototype Tuberculosis Antigen." PloS one (2015).

Epitope: Ag85A Peptide 11-30
Chemical type: Peptide from protein
Sequence: LQVPSPSMGRDIKVQFQSGG
Position: 54-73
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 11-30
Chemical type: Peptide from protein
Sequence: LQVPSPSMGRDIKVQFQSGG
Position: 54-73
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 21-40
Chemical type: Peptide from protein
Sequence: DIKVQFQSGGANSPALYLLD
Position: 64-83
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 21-40
Chemical type: Peptide from protein
Sequence: DIKVQFQSGGANSPALYLLD
Position: 64-83
Source molecule (GenBank): P17944
Assay category: TCell
Result: Negative
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 35-53
Chemical type: Peptide from protein
Sequence: ALYLLDGLRAQDDFSGWDI
Position: 78-96
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 35-53
Chemical type: Peptide from protein
Sequence: ALYLLDGLRAQDDFSGWDI
Position: 78-96
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 51-70
Chemical type: Peptide from protein
Sequence: WDINTPAFEWYDQSGLSVVM
Position: 94-113
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 51-70
Chemical type: Peptide from protein
Sequence: WDINTPAFEWYDQSGLSVVM
Position: 94-113
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 61-80
Chemical type: Peptide from protein
Sequence: YDQSGLSVVMPVGGQSSFYS
Position: 104-123
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 61-80
Chemical type: Peptide from protein
Sequence: YDQSGLSVVMPVGGQSSFYS
Position: 104-123
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 71-90
Chemical type: Peptide from protein
Sequence: PVGGQSSFYSDWYQPACGKA
Position: 114-133
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 71-90
Chemical type: Peptide from protein
Sequence: PVGGQSSFYSDWYQPACGKA
Position: 114-133
Source molecule (GenBank): P17944
Assay category: TCell
Result: Negative
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 81-100
Chemical type: Peptide from protein
Sequence: DWYQPACGKAGCQTYKWETF
Position: 124-143
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 81-100
Chemical type: Peptide from protein
Sequence: DWYQPACGKAGCQTYKWETF
Position: 124-143
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 91-108
Chemical type: Peptide from protein
Sequence: GCQTYKWETFLTSELPQW
Position: 131-148
Source molecule (GenBank): AAO62005.1
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 91-108
Chemical type: Peptide from protein
Sequence: GCQTYKWETFLTSELPQW
Position: 131-148
Source molecule (GenBank): AAO62005.1
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 101-120
Chemical type: Peptide from protein
Sequence: LTSELPGWLQANRHVKPTGS
Position: 144-163
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive-Low
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 101-120
Chemical type: Peptide from protein
Sequence: LTSELPGWLQANRHVKPTGS
Position: 144-163
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 121-140
Chemical type: Peptide from protein
Sequence: AVVGLSMAASSALTLAIYHP
Position: 164-183
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 121-140
Chemical type: Peptide from protein
Sequence: AVVGLSMAASSALTLAIYHP
Position: 164-183
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 131-150
Chemical type: Peptide from protein
Sequence: SALTLAIYHPQQFVYAGAMS
Position: 174-193
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 131-150
Chemical type: Peptide from protein
Sequence: SALTLAIYHPQQFVYAGAMS
Position: 174-193
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive-High
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 141-160
Chemical type: Peptide from protein
Sequence: QQFVYAGAMSGLLDPSQAMG
Position: 184-203
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 141-160
Chemical type: Peptide from protein
Sequence: QQFVYAGAMSGLLDPSQAMG
Position: 184-203
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 151-170
Chemical type: Peptide from protein
Sequence: GLLDPSQAMGPTLIGLAMGD
Position: 194-213
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 151-170
Chemical type: Peptide from protein
Sequence: GLLDPSQAMGPTLIGLAMGD
Position: 194-213
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 161-180
Chemical type: Peptide from protein
Sequence: PTLIGLAMGDAGGYKASDMW
Position: 204-223
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 161-180
Chemical type: Peptide from protein
Sequence: PTLIGLAMGDAGGYKASDMW
Position: 204-223
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 171-190
Chemical type: Peptide from protein
Sequence: AGGYKASDMWGPKEDPAWQR
Position: 214-233
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 171-190
Chemical type: Peptide from protein
Sequence: AGGYKASDMWGPKEDPAWQR
Position: 214-233
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 181-200
Chemical type: Peptide from protein
Sequence: GPKEDPAWQRNDPLLNVGKL
Position: 224-243
Source molecule (GenBank): P17944
Assay category: TCell
Result: Negative
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 181-200
Chemical type: Peptide from protein
Sequence: GPKEDPAWQRNDPLLNVGKL
Position: 224-243
Source molecule (GenBank): P17944
Assay category: TCell
Result: Negative
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 191-210
Chemical type: Peptide from protein
Sequence: NDPLLNVGKLIANNTRVWVY
Position: 234-253
Source molecule (GenBank): P17944
Assay category: TCell
Result: Negative
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 191-210
Chemical type: Peptide from protein
Sequence: NDPLLNVGKLIANNTRVWVY
Position: 234-253
Source molecule (GenBank): P17944
Assay category: TCell
Result: Negative
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 201-220
Chemical type: Peptide from protein
Sequence: IANNTRVWVYCGNGKPSDLG
Position: 244-263
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 201-220
Chemical type: Peptide from protein
Sequence: IANNTRVWVYCGNGKPSDLG
Position: 244-263
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 211-230
Chemical type: Peptide from protein
Sequence: CGNGKPSDLGGNNLPAKFLE
Position: 254-273
Source molecule (GenBank): P17944
Assay category: TCell
Result: Negative
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 211-230
Chemical type: Peptide from protein
Sequence: CGNGKPSDLGGNNLPAKFLE
Position: 254-273
Source molecule (GenBank): P17944
Assay category: TCell
Result: Negative
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 221-240
Chemical type: Peptide from protein
Sequence: GNNLPAKFLEGFVRTSNIKF
Position: 264-283
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 221-240
Chemical type: Peptide from protein
Sequence: GNNLPAKFLEGFVRTSNIKF
Position: 264-283
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 231-250
Chemical type: Peptide from protein
Sequence: GFVRTSNIKFQDAYNAGGGH
Position: 274-293
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 231-250
Chemical type: Peptide from protein
Sequence: GFVRTSNIKFQDAYNAGGGH
Position: 274-293
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 241-260
Chemical type: Peptide from protein
Sequence: QDAYNAGGGHNGVFDFPDSG
Position: 284-303
Source molecule (GenBank): P17944
Assay category: TCell
Result: Negative
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 241-260
Chemical type: Peptide from protein
Sequence: QDAYNAGGGHNGVFDFPDSG
Position: 284-303
Source molecule (GenBank): P17944
Assay category: TCell
Result: Negative
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 251-270
Chemical type: Peptide from protein
Sequence: NGVFDFPDSGTHSWEYWGAQ
Position: 294-313
Source molecule (GenBank): P17944
Assay category: TCell
Result: Negative
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 251-270
Chemical type: Peptide from protein
Sequence: NGVFDFPDSGTHSWEYWGAQ
Position: 294-313
Source molecule (GenBank): P17944
Assay category: TCell
Result: Negative
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 261-280
Chemical type: Peptide from protein
Sequence: THSWEYWGAQLNAMKPDLQR
Position: 304-323
Source molecule (GenBank): P17944
Assay category: TCell
Result: Negative
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 261-280
Chemical type: Peptide from protein
Sequence: THSWEYWGAQLNAMKPDLQR
Position: 304-323
Source molecule (GenBank): P17944
Assay category: TCell
Result: Positive
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 275-294
Chemical type: Peptide from protein
Sequence: KPDLQRALGATPNTGPAPQG
Position: 318-337
Source molecule (GenBank): P17944
Assay category: TCell
Result: Negative
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 275-294
Chemical type: Peptide from protein
Sequence: KPDLQRALGATPNTGPAPQG
Position: 318-337
Source molecule (GenBank): P17944
Assay category: TCell
Result: Negative
Host:  10 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Ag85A Peptide 129-137
Chemical type: Peptide from protein
Sequence: ASSALTLAI
Position: 172-180
Source molecule (GenBank): P17944
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 10.0

Epitope: Ag85A Peptide 135-143
Chemical type: Peptide from protein
Sequence: LAIYHPQQF
Position: 178-186
Source molecule (GenBank): P17944
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 20000.0

Epitope: Ag85A Peptide 129-138
Chemical type: Peptide from protein
Sequence: ASSALTLAIY
Position: 172-181
Source molecule (GenBank): P17944
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 1971.0

Epitope: Ag85A Peptide 136-145
Chemical type: Peptide from protein
Sequence: AIYHPQQFVY
Position: 179-188
Source molecule (GenBank): P17944
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 5.0

Epitope: Ag85A Peptide 130-138
Chemical type: Peptide from protein
Sequence: SSAMILAAY
Position: 130-138
Source molecule (GenBank): AAF13448.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 106.0

Epitope: Ag85A Peptide 129-138
Chemical type: Peptide from protein
Sequence: GSSAMILAAY
Position: 129-138
Source molecule (GenBank): AAF13448.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 7755.0

Epitope: Ag85A Peptide 134-143
Chemical type: Peptide from protein
Sequence: ILAAYHPQQF
Position: 134-143
Source molecule (GenBank): AAF13448.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 6043.0