Reference: Aïsha Callebaut; Perrin Guyer; Rita Derua; Mijke Buitinga; Anthony Manganaro; Xiaoyan Yi; Fernanda Marques Câmara Sodré; Saurabh Vig; Mara Suleiman; Piero Marchetti; Decio L Eizirik; Sally C Kent; Chantal Mathieu; Eddie A James; Lut Overbergh. "CD4+ T Cells From Individuals With Type 1 Diabetes Respond to a Novel Class of Deamidated Peptides Formed in Pancreatic Islets." Diabetes (2024).

Epitope: GRP78 445-459 E449
Chemical type: Peptide from protein
Sequence: TKKSQIFSTASDNQP
Position: 445-459
Source molecule (GenBank): P11021.2
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GRP78 445-459 E449
Chemical type: Peptide from protein
Sequence: TKKSQIFSTASDNQP
Position: 445-459
Source molecule (GenBank): P11021.2
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1480.0

Epitope: HSPB1 166-180 E175
Chemical type: Peptide from protein
Sequence: EAPMPKLATQSNEIT
Position: 166-180
Source molecule (GenBank): P04792.2
Assay category: TCell
Result: Positive-High
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HSPB1 166-180 E175
Chemical type: Peptide from protein
Sequence: EAPMPKLATQSNEIT
Position: 166-180
Source molecule (GenBank): P04792.2
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 41810.0

Epitope: P4HB 188-202 E197
Chemical type: Peptide from protein
Sequence: SNSDVFSKYQLDKDG
Position: 188-202
Source molecule (GenBank): P07237.3
Assay category: TCell
Result: Positive-High
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: P4HB 188-202 E197
Chemical type: Peptide from protein
Sequence: SNSDVFSKYQLDKDG
Position: 188-202
Source molecule (GenBank): P07237.3
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 19510.0

Epitope: P4HB 193-207 E197
Chemical type: Peptide from protein
Sequence: FSKYQLDKDGVVLFK
Position: 193-207
Source molecule (GenBank): P07237.3
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P4HB 193-207 E197
Chemical type: Peptide from protein
Sequence: FSKYQLDKDGVVLFK
Position: 193-207
Source molecule (GenBank): P07237.3
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P4HB 193-207 E197
Chemical type: Peptide from protein
Sequence: FSKYQLDKDGVVLFK
Position: 193-207
Source molecule (GenBank): P07237.3
Assay category: TCell
Result: Positive-High
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: P4HB 193-207 E197
Chemical type: Peptide from protein
Sequence: FSKYQLDKDGVVLFK
Position: 193-207
Source molecule (GenBank): P07237.3
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: P4HB 193-207 E197
Chemical type: Peptide from protein
Sequence: FSKYQLDKDGVVLFK
Position: 193-207
Source molecule (GenBank): P07237.3
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: P4HB 193-207 E197
Chemical type: Peptide from protein
Sequence: FSKYQLDKDGVVLFK
Position: 193-207
Source molecule (GenBank): P07237.3
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 4390.0

Epitope: YWHAZ 138-152 E144E146
Chemical type: Peptide from protein
Sequence: KKGIVDQSQQAYQEA
Position: 138-152
Source molecule (GenBank): P63104.1
Assay category: TCell
Result: Positive-High
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: YWHAZ 138-152 E144E146
Chemical type: Peptide from protein
Sequence: KKGIVDQSQQAYQEA
Position: 138-152
Source molecule (GenBank): P63104.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 30220.0

Epitope: GRP78 447-466 D457
Chemical type: Peptide from protein
Sequence: KSQIFSTASDNQPTV
Position: 447-461
Source molecule (GenBank): P11021.2
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GRP78 447-466 D457
Chemical type: Peptide from protein
Sequence: KSQIFSTASDNQPTV
Position: 447-461
Source molecule (GenBank): P11021.2
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GRP78 447-466 D457
Chemical type: Peptide from protein
Sequence: KSQIFSTASDNQPTV
Position: 447-461
Source molecule (GenBank): P11021.2
Assay category: TCell
Result: Positive-High
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GRP78 447-466 D457
Chemical type: Peptide from protein
Sequence: KSQIFSTASDNQPTV
Position: 447-461
Source molecule (GenBank): P11021.2
Assay category: TCell
Result: Positive-High
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GRP78 447-466 D457
Chemical type: Peptide from protein
Sequence: KSQIFSTASDNQPTV
Position: 447-461
Source molecule (GenBank): P11021.2
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GRP78 447-466 D457
Chemical type: Peptide from protein
Sequence: KSQIFSTASDNQPTV
Position: 447-461
Source molecule (GenBank): P11021.2
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 10010.0

Epitope: GRP94 114-133 D124
Chemical type: Peptide from protein
Sequence: LISLTDENALSGNEE
Position: 117-131
Source molecule (GenBank): P14625.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GRP94 114-133 D124
Chemical type: Peptide from protein
Sequence: LISLTDENALSGNEE
Position: 117-131
Source molecule (GenBank): P14625.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GRP94 114-133 D124
Chemical type: Peptide from protein
Sequence: LISLTDENALSGNEE
Position: 117-131
Source molecule (GenBank): P14625.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GRP94 114-133 D124
Chemical type: Peptide from protein
Sequence: LISLTDENALSGNEE
Position: 117-131
Source molecule (GenBank): P14625.1
Assay category: TCell
Result: Positive-High
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GRP94 114-133 D124
Chemical type: Peptide from protein
Sequence: LISLTDENALSGNEE
Position: 117-131
Source molecule (GenBank): P14625.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GRP94 114-133 D124
Chemical type: Peptide from protein
Sequence: LISLTDENALSGNEE
Position: 117-131
Source molecule (GenBank): P14625.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 9110.0

Epitope: GRP94 328-347 D337
Chemical type: Peptide from protein
Sequence: VWDWELMNDIKPIWQ
Position: 330-344
Source molecule (GenBank): P14625.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GRP94 328-347 D337
Chemical type: Peptide from protein
Sequence: VWDWELMNDIKPIWQ
Position: 330-344
Source molecule (GenBank): P14625.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GRP94 328-347 D337
Chemical type: Peptide from protein
Sequence: VWDWELMNDIKPIWQ
Position: 330-344
Source molecule (GenBank): P14625.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GRP94 328-347 D337
Chemical type: Peptide from protein
Sequence: VWDWELMNDIKPIWQ
Position: 330-344
Source molecule (GenBank): P14625.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GRP94 328-347 D337
Chemical type: Peptide from protein
Sequence: VWDWELMNDIKPIWQ
Position: 330-344
Source molecule (GenBank): P14625.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 28020.0

Epitope: HSPA9 139-158 D149
Chemical type: Peptide from protein
Sequence: NVPFKIVRASNGDAW
Position: 139-153
Source molecule (GenBank): P38646.2
Assay category: TCell
Result: Positive-High
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HSPA9 139-158 D149
Chemical type: Peptide from protein
Sequence: NVPFKIVRASNGDAW
Position: 139-153
Source molecule (GenBank): P38646.2
Assay category: TCell
Result: Positive-High
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: HSPA9 139-158 D149
Chemical type: Peptide from protein
Sequence: NVPFKIVRASNGDAW
Position: 139-153
Source molecule (GenBank): P38646.2
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: HSPA9 139-158 D149
Chemical type: Peptide from protein
Sequence: NVPFKIVRASNGDAW
Position: 139-153
Source molecule (GenBank): P38646.2
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HSPA9 139-158 D149
Chemical type: Peptide from protein
Sequence: NVPFKIVRASNGDAW
Position: 139-153
Source molecule (GenBank): P38646.2
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HSPA9 139-158 D149
Chemical type: Peptide from protein
Sequence: NVPFKIVRASNGDAW
Position: 139-153
Source molecule (GenBank): P38646.2
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 3350.0

Epitope: Deamidated peptide 10
Chemical type: Peptide from protein
Sequence: LKELQDLALQGAKER
Position: 76-90
Source molecule (GenBank): P10645.7
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: Deamidated peptide 12
Chemical type: Peptide from protein
Sequence: ELSYAKNGQDLGVAF
Position: 418-432
Source molecule (GenBank): Q00839.6
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: Deamidated peptide 13
Chemical type: Peptide from protein
Sequence: RQEMQEVQSSRSGRG
Position: 190-204
Source molecule (GenBank): P22626.2
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: Deamidated peptide 16
Chemical type: Peptide from protein
Sequence: TYSCVGVFKNGRVEI
Position: 38-52
Source molecule (GenBank): P11021.2
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: Deamidated peptide 17
Chemical type: Peptide from protein
Sequence: ERLIGDAKNQLTSNP
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: Deamidated peptide 18
Chemical type: Peptide from protein
Sequence: GDAKNQLTSNPENT
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: Deamidated peptide 20
Chemical type: Peptide from protein
Sequence: TPAYNDAQRQATKDA
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: Deamidated peptide 21
Chemical type: Peptide from protein
Sequence: RAKFEELNMDLFRST
Position: 324-338
Source molecule (GenBank): P11021.2
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: Deamidated peptide 22
Chemical type: Peptide from protein
Sequence: QQLVKEFFNGKEPSR
Position: 372-386
Source molecule (GenBank): P11021.2
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: Deamidated peptide 23
Chemical type: Peptide from protein
Sequence: KITITNDQNRLTPEE
Position: 523-537
Source molecule (GenBank): P11021.2
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: Deamidated peptide 24
Chemical type: Peptide from protein
Sequence: KERIDTRNELESYAY
Position: 556-570
Source molecule (GenBank): P11021.2
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: Deamidated peptide 25
Chemical type: Peptide from protein
Sequence: EAIQLDGLNASQIR
Position: 54-67
Source molecule (GenBank): P14625.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: Deamidated peptide 27
Chemical type: Peptide from protein
Sequence: EIFLRELISNASDA
Position: 37-50
Source molecule (GenBank): P08238.4
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: Deamidated peptide 28
Chemical type: Peptide from protein
Sequence: EKEFEPLLNWMKDKA
Position: 612-626
Source molecule (GenBank): P14625.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: Deamidated peptide 29
Chemical type: Peptide from protein
Sequence: GKDISTNYYASQKQ
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: Deamidated peptide 30
Chemical type: Peptide from protein
Sequence: GKDISTNYYASQKKT
Position: 670-684
Source molecule (GenBank): P14625.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: Deamidated peptide 31
Chemical type: Peptide from protein
Sequence: KRQAVTNPNNTFYAT
Position: 106-120
Source molecule (GenBank): P38646.2
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: Deamidated peptide 33
Chemical type: Peptide from protein
Sequence: KGVFEVKSTNGDTF
Position: 259-272
Source molecule (GenBank): P38646.2
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: Deamidated peptide 34
Chemical type: Peptide from protein
Sequence: PVIKSQPVPKNNKG
Position: 512-525
Source molecule (GenBank): P13667.2
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: Deamidated peptide 35
Chemical type: Peptide from protein
Sequence: SQPVPKNNKGPVKVV
Position: 516-530
Source molecule (GenBank): P13667.2
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: Deamidated peptide 36
Chemical type: Peptide from protein
Sequence: CKQLEPVYNSLAKKY
Position: 558-572
Source molecule (GenBank): P13667.2
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: Deamidated peptide 37
Chemical type: Peptide from protein
Sequence: SVPLQQKENGDESHL
Position: 85-99
Source molecule (GenBank): P13521.2
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: Deamidated peptide 38
Chemical type: Peptide from protein
Sequence: IEMLKTGEKPNGSV
Position: 366-379
Source molecule (GenBank): P13521.2
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: Deamidated peptide 39
Chemical type: Peptide from protein
Sequence: PNPYNQEKVLPRLPY
Position: 454-468
Source molecule (GenBank): P13521.2
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: Deamidated peptide 40
Chemical type: Peptide from protein
Sequence: QMAYENLNDKDQELG
Position: 491-505
Source molecule (GenBank): P13521.2
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: WT Peptide 41
Chemical type: Peptide from protein
Sequence: FSKYQLDKDGVVLFK
Position: 193-207
Source molecule (GenBank): P07237.3
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: WT Peptide 41
Chemical type: Peptide from protein
Sequence: FSKYQLDKDGVVLFK
Position: 193-207
Source molecule (GenBank): P07237.3
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 3230.0

Epitope: WT Peptide 42
Chemical type: Peptide from protein
Sequence: KSQIFSTASDNQPTVTIKVY
Position: 447-466
Source molecule (GenBank): P11021.2
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: WT Peptide 42
Chemical type: Peptide from protein
Sequence: KSQIFSTASDNQPTVTIKVY
Position: 447-466
Source molecule (GenBank): P11021.2
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 460.0

Epitope: WT Peptide 43
Chemical type: Peptide from protein
Sequence: KTVWDWELMNDIKPIWQRPS
Position: 328-347
Source molecule (GenBank): P14625.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 100000.0

Epitope: WT Peptide 44
Chemical type: Peptide from protein
Sequence: KIRLISLTDENALSGNEELT
Position: 114-133
Source molecule (GenBank): P14625.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: WT Peptide 44
Chemical type: Peptide from protein
Sequence: KIRLISLTDENALSGNEELT
Position: 114-133
Source molecule (GenBank): P14625.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1120.0

Epitope: WT Peptide 45
Chemical type: Peptide from protein
Sequence: NVPFKIVRASNGDAWVEAHG
Position: 139-158
Source molecule (GenBank): P38646.2
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: WT Peptide 45
Chemical type: Peptide from protein
Sequence: NVPFKIVRASNGDAWVEAHG
Position: 139-158
Source molecule (GenBank): P38646.2
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 120.0