Reference: Magdalini Moutaftsi; Huynh-Hoa Bui; Bjoern Peters; John Sidney; Shahram Salek-Ardakani; Carla Oseroff; Valerie Pasquetto; Shane Crotty; Michael Croft; Elliot J Lefkowitz; Howard Grey; Alessandro Sette. "Vaccinia virus-specific CD4+ T cell responses target a set of antigens largely distinct from those targeted by CD8+ T cell responses." Journal of immunology (Baltimore, Md. : 1950) (2007).

Epitope: A18R
Chemical type: Peptide from protein
Sequence: PKGFYASPSVKTSLV
Position: 46-60
Source molecule (GenBank): P16712.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: A18R
Chemical type: Peptide from protein
Sequence: PKGFYASPSVKTSLV
Position: 46-60
Source molecule (GenBank): P16712.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 83.0

Epitope: A20R
Chemical type: Peptide from protein
Sequence: DNIFIPSVITKSGKK
Position: 230-244
Source molecule (GenBank): P68710.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: A20R
Chemical type: Peptide from protein
Sequence: DNIFIPSVITKSGKK
Position: 230-244
Source molecule (GenBank): P68710.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 332.0

Epitope: A28L
Chemical type: Peptide from protein
Sequence: FFIVVATAAVCLLFI
Position: 7-21
Source molecule (GenBank): P68633.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: A28L
Chemical type: Peptide from protein
Sequence: FFIVVATAAVCLLFI
Position: 7-21
Source molecule (GenBank): P68633.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 4081.0

Epitope: D13L
Chemical type: Peptide from protein
Sequence: PKIFFRPTTITANVS
Position: 483-497
Source molecule (GenBank): P68440.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: D13L
Chemical type: Peptide from protein
Sequence: PKIFFRPTTITANVS
Position: 483-497
Source molecule (GenBank): P68440.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 19.0

Epitope: D8L
Chemical type: Peptide from protein
Sequence: GEIIRAATTSPAREN
Position: 235-249
Source molecule (GenBank): P04195.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: D8L
Chemical type: Peptide from protein
Sequence: GEIIRAATTSPAREN
Position: 235-249
Source molecule (GenBank): P04195.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 122.0

Epitope: E1L
Chemical type: Peptide from protein
Sequence: VLTIKAPNVISSKIS
Position: 114-128
Source molecule (GenBank): P23371.2
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: E1L
Chemical type: Peptide from protein
Sequence: VLTIKAPNVISSKIS
Position: 114-128
Source molecule (GenBank): P23371.2
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 188.0

Epitope: E9L
Chemical type: Peptide from protein
Sequence: PSVFINPISHTSYCY
Position: 176-190
Source molecule (GenBank): P06856.4
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: E9L
Chemical type: Peptide from protein
Sequence: PSVFINPISHTSYCY
Position: 176-190
Source molecule (GenBank): P06856.4
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 77.0

Epitope: F15L
Chemical type: Peptide from protein
Sequence: TPRYIPSTSISSSNI
Position: 41-55
Source molecule (GenBank): AAO89333.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: F15L
Chemical type: Peptide from protein
Sequence: TPRYIPSTSISSSNI
Position: 41-55
Source molecule (GenBank): AAO89333.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 353.0

Epitope: H3L
Chemical type: Peptide from protein
Sequence: PGVMYAFTTPLISFF
Position: 269-283
Source molecule (GenBank): P07240.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H3L
Chemical type: Peptide from protein
Sequence: PGVMYAFTTPLISFF
Position: 269-283
Source molecule (GenBank): P07240.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 5.4

Epitope: I1L (21-35)
Chemical type: Peptide from protein
Sequence: LKAYFTAKINEMVDE
Position: 18-32
Source molecule (GenBank): P16714.2
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: I1L (21-35)
Chemical type: Peptide from protein
Sequence: LKAYFTAKINEMVDE
Position: 18-32
Source molecule (GenBank): P16714.2
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 362.0

Epitope: J4R
Chemical type: Peptide from protein
Sequence: DDDYGEPIIITSYLQ
Position: 75-89
Source molecule (GenBank): P68609.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: J4R
Chemical type: Peptide from protein
Sequence: DDDYGEPIIITSYLQ
Position: 75-89
Source molecule (GenBank): P68609.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 2266.0

Epitope: L4R
Chemical type: Peptide from protein
Sequence: ISKYAGINILNVYSP
Position: 173-187
Source molecule (GenBank): P03295.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: L4R
Chemical type: Peptide from protein
Sequence: ISKYAGINILNVYSP
Position: 173-187
Source molecule (GenBank): P03295.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 128.0