Reference: R A Court; K Sitte; J P Opdebeeck; I J East. "Mapping the T cell epitopes of the Babesia bovis antigen 12D3: implications for vaccine design." Parasite immunology (1998).

Epitope: 12D3 (35-43)
Chemical type: Peptide from protein
Sequence: KLVPDSSNI
Position: 35-43
Source molecule (GenBank): XP_001610737.1
Assay category: TCell
Result: Positive
MHC allele types: 488
Host: M 15-18 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 12D3 (61-80)
Chemical type: Peptide from protein
Sequence: TMQSGVYLVNPYAQRCPIGK
Assay category: TCell
Result: Positive
MHC allele types: 488
Host: M 15-18 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 12D3 (211-230)
Chemical type: Peptide from protein
Sequence: IVELEDDRVVSVIASGYVNG
Position: 211-230
Source molecule (GenBank): XP_001610737.1
Assay category: TCell
Result: Positive
MHC allele types: 488
Host: M 15-18 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 12D3 (266-275)
Chemical type: Peptide from protein
Sequence: KIKTDDQLML
Position: 266-275
Source molecule (GenBank): XP_001610737.1
Assay category: TCell
Result: Positive
MHC allele types: 488
Host: M 15-18 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 12D3 (281-300)
Chemical type: Peptide from protein
Sequence: ANDIIAFTTTEANPSGLTKI
Position: 281-300
Source molecule (GenBank): XP_001610737.1
Assay category: TCell
Result: Positive
MHC allele types: 488
Host: M 15-18 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 12D3 (1-20)
Chemical type: Peptide from protein
Sequence: MLATRFVFSLCLFVAHQGVN
Assay category: TCell
Result: Negative
Host: M 15-18 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 12D3 (11-30)
Chemical type: Peptide from protein
Sequence: CLFVAHQGVNAFFYRAVPMY
Position: 11-30
Source molecule (GenBank): XP_001610737.1
Assay category: TCell
Result: Negative
Host: M 15-18 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 12D3 (21-40)
Chemical type: Peptide from protein
Sequence: AFFYRAVPMYDQNGKLVPDS
Position: 21-40
Source molecule (GenBank): XP_001610737.1
Assay category: TCell
Result: Negative
Host: M 15-18 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 12D3 (51-70)
Chemical type: Peptide from protein
Sequence: EATGVGRICSTMQSGIYLIN
Position: 51-70
Source molecule (GenBank): XP_001610737.1
Assay category: TCell
Result: Negative
Host: M 15-18 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 12D3 (81-100)
Chemical type: Peptide from protein
Sequence: SKCGPEHDSTLGKCAMYSGC
Position: 81-100
Source molecule (GenBank): XP_001610737.1
Assay category: TCell
Result: Negative
Host: M 15-18 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 12D3 (91-110)
Chemical type: Peptide from protein
Sequence: LGKCAMYSGCYTISNDFRAC
Position: 91-110
Source molecule (GenBank): XP_001610737.1
Assay category: TCell
Result: Negative
Host: M 15-18 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 12D3 (101-120)
Chemical type: Peptide from protein
Sequence: YTISNDFRACSCLPDYFGNP
Position: 101-120
Source molecule (GenBank): XP_001610737.1
Assay category: TCell
Result: Negative
Host: M 15-18 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 12D3 (111-130)
Chemical type: Peptide from protein
Sequence: SCLPDYFGNPYKQCFRHCDT
Position: 111-130
Source molecule (GenBank): XP_001610737.1
Assay category: TCell
Result: Negative
Host: M 15-18 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 12D3 (121-140)
Chemical type: Peptide from protein
Sequence: YKQCFRHCDTDLDCPSPYAE
Position: 121-140
Source molecule (GenBank): XP_001610737.1
Assay category: TCell
Result: Negative
Host: M 15-18 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 12D3 (131-150)
Chemical type: Peptide from protein
Sequence: DLDCPSPYAECRMDAGETIK
Position: 131-150
Source molecule (GenBank): XP_001610737.1
Assay category: TCell
Result: Negative
Host: M 15-18 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 12D3 (151-170)
Chemical type: Peptide from protein
Sequence: RCKCRAGCPGDGVICKPDKI
Position: 151-170
Source molecule (GenBank): XP_001610737.1
Assay category: TCell
Result: Negative
Host: M 15-18 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 12D3 (161-180)
Chemical type: Peptide from protein
Sequence: DGVICKPDKICTDLAEDSEA
Position: 161-180
Source molecule (GenBank): XP_001610737.1
Assay category: TCell
Result: Negative
Host: M 15-18 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 12D3 (171-190)
Chemical type: Peptide from protein
Sequence: CTDLAEDSEAHRRCLQTGIT
Position: 171-190
Source molecule (GenBank): XP_001610737.1
Assay category: TCell
Result: Negative
Host: M 15-18 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 12D3 (181-200)
Chemical type: Peptide from protein
Sequence: HRRCLQTGITDKTYVCDDGY
Position: 181-200
Source molecule (GenBank): XP_001610737.1
Assay category: TCell
Result: Negative
Host: M 15-18 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 12D3 (231-250)
Chemical type: Peptide from protein
Sequence: SRIDIGNCFSIEINKDNGMS
Position: 231-250
Source molecule (GenBank): XP_001610737.1
Assay category: TCell
Result: Negative
Host: M 15-18 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 12D3 (241-260)
Chemical type: Peptide from protein
Sequence: IEINKDNGMSYFYQLNSGDP
Position: 241-260
Source molecule (GenBank): XP_001610737.1
Assay category: TCell
Result: Negative
Host: M 15-18 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 12D3 (321-340)
Chemical type: Peptide from protein
Sequence: TGAKEHPSGVRVEVKELETS
Position: 321-340
Source molecule (GenBank): XP_001610737.1
Assay category: TCell
Result: Negative
Host: M 15-18 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo