Reference: Stefania Picascia; John Sidney; Alessandra Camarca; Giuseppe Mazzarella; Nicola Giardullo; Luigi Greco; Renata Auricchio; Salvatore Auricchio; Riccardo Troncone; Alessandro Sette; Carmen Gianfrani. "Gliadin-Specific CD8<sup>+</sup> T Cell Responses Restricted by HLA Class I A*0101 and B*0801 Molecules in Celiac Disease Patients." Journal of immunology (Baltimore, Md. : 1950) (2017).

Epitope: alpha gliadin 158-167
Chemical type: Peptide from protein
Sequence: SSQVLQQSTY
Position: 158-167
Source molecule (GenBank): Q9M4L6.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: alpha gliadin 158-167
Chemical type: Peptide from protein
Sequence: SSQVLQQSTY
Position: 158-167
Source molecule (GenBank): Q9M4L6.1
Assay category: TCell
Result: Positive
Host:  5-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: alpha gliadin 158-167
Chemical type: Peptide from protein
Sequence: SSQVLQQSTY
Position: 158-167
Source molecule (GenBank): Q9M4L6.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 100000.0

Epitope: alpha gliadin 158-167
Chemical type: Peptide from protein
Sequence: SSQVLQQSTY
Position: 158-167
Source molecule (GenBank): Q9M4L6.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 103.0

Epitope: omega gliadin 11-19
Chemical type: Peptide from protein
Sequence: AMAMKIATA
Position: 11-19
Source molecule (GenBank): AAG17702.1
Assay category: TCell
Result: Positive
Host:  8-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: omega gliadin 11-19
Chemical type: Peptide from protein
Sequence: AMAMKIATA
Position: 11-19
Source molecule (GenBank): AAG17702.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: omega gliadin 11-19
Chemical type: Peptide from protein
Sequence: AMAMKIATA
Position: 11-19
Source molecule (GenBank): AAG17702.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: omega gliadin 11-19
Chemical type: Peptide from protein
Sequence: AMAMKIATA
Position: 11-19
Source molecule (GenBank): AAG17702.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 48.0

Epitope: omega gliadin 11-19
Chemical type: Peptide from protein
Sequence: AMAMKIATA
Position: 11-19
Source molecule (GenBank): AAG17702.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 100000.0

Epitope: omega gliadin 22-30
Chemical type: Peptide from protein
Sequence: LLSPRGKEL
Position: 22-30
Source molecule (GenBank): Q402I5.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: omega gliadin 22-30
Chemical type: Peptide from protein
Sequence: LLSPRGKEL
Position: 22-30
Source molecule (GenBank): Q402I5.1
Assay category: TCell
Result: Positive
Host:  8-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: omega gliadin 22-30
Chemical type: Peptide from protein
Sequence: LLSPRGKEL
Position: 22-30
Source molecule (GenBank): Q402I5.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 100000.0

Epitope: omega gliadin 22-30
Chemical type: Peptide from protein
Sequence: LLSPRGKEL
Position: 22-30
Source molecule (GenBank): Q402I5.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 91.0

Epitope: gamma gliadin 287-295
Chemical type: Peptide from protein
Sequence: LEAIRSLVL
Position: 287-295
Source molecule (GenBank): P08453.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: gamma gliadin 287-295
Chemical type: Peptide from protein
Sequence: LEAIRSLVL
Position: 287-295
Source molecule (GenBank): P08453.1
Assay category: TCell
Result: Positive
Host:  8-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: gamma gliadin 287-295
Chemical type: Peptide from protein
Sequence: LEAIRSLVL
Position: 287-295
Source molecule (GenBank): P08453.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 100000.0

Epitope: gamma gliadin 287-295
Chemical type: Peptide from protein
Sequence: LEAIRSLVL
Position: 287-295
Source molecule (GenBank): P08453.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 303.0

Epitope: alpha gliadin 1-9
Chemical type: Peptide from protein
Sequence: MKTFLILAL
Position: 1-9
Source molecule (GenBank): P18573.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: alpha gliadin 1-9
Chemical type: Peptide from protein
Sequence: MKTFLILAL
Position: 1-9
Source molecule (GenBank): P18573.1
Assay category: TCell
Result: Positive
Host:  8-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: alpha gliadin 1-9
Chemical type: Peptide from protein
Sequence: MKTFLILAL
Position: 1-9
Source molecule (GenBank): P18573.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 100000.0

Epitope: alpha gliadin 1-9
Chemical type: Peptide from protein
Sequence: MKTFLILAL
Position: 1-9
Source molecule (GenBank): P18573.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 223.0

Epitope: gamma gliadin 224-232
Chemical type: Peptide from protein
Sequence: QGMHILLPL
Position: 224-232
Source molecule (GenBank): P08079.1
Assay category: TCell
Result: Positive
Host:  8-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: gamma gliadin 224-232
Chemical type: Peptide from protein
Sequence: QGMHILLPL
Position: 224-232
Source molecule (GenBank): P08079.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: gamma gliadin 224-232
Chemical type: Peptide from protein
Sequence: QGMHILLPL
Position: 224-232
Source molecule (GenBank): P08079.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: gamma gliadin 224-232
Chemical type: Peptide from protein
Sequence: QGMHILLPL
Position: 224-232
Source molecule (GenBank): P08079.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 100000.0

Epitope: gamma gliadin 224-232
Chemical type: Peptide from protein
Sequence: QGMHILLPL
Position: 224-232
Source molecule (GenBank): P08079.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 73.0

Epitope: gamma gliadin 203-211
Chemical type: Peptide from protein
Sequence: CAAIHTIIH
Position: 203-211
Source molecule (GenBank): P08079.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: gamma gliadin 203-211
Chemical type: Peptide from protein
Sequence: CAAIHTIIH
Position: 203-211
Source molecule (GenBank): P08079.1
Assay category: TCell
Result: Positive
Host:  8-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: gamma gliadin 203-211
Chemical type: Peptide from protein
Sequence: CAAIHTIIH
Position: 203-211
Source molecule (GenBank): P08079.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 100000.0

Epitope: gamma gliadin 203-211
Chemical type: Peptide from protein
Sequence: CAAIHTIIH
Position: 203-211
Source molecule (GenBank): P08079.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 465.0

Epitope: gamma gliadin 213-222
Chemical type: Peptide from protein
Sequence: VMRQQCCQQL
Position: 213-222
Source molecule (GenBank): P08453.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: gamma gliadin 213-222
Chemical type: Peptide from protein
Sequence: VMRQQCCQQL
Position: 213-222
Source molecule (GenBank): P08453.1
Assay category: TCell
Result: Positive
Host:  8-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: gamma gliadin 213-222
Chemical type: Peptide from protein
Sequence: VMRQQCCQQL
Position: 213-222
Source molecule (GenBank): P08453.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 100000.0

Epitope: gamma gliadin 213-222
Chemical type: Peptide from protein
Sequence: VMRQQCCQQL
Position: 213-222
Source molecule (GenBank): P08453.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 162.0

Epitope: gamma gliadin 286-294
Chemical type: Peptide from protein
Sequence: QLEAIRSLV
Position: 286-294
Source molecule (GenBank): P08453.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: gamma gliadin 286-294
Chemical type: Peptide from protein
Sequence: QLEAIRSLV
Position: 286-294
Source molecule (GenBank): P08453.1
Assay category: TCell
Result: Positive
Host:  8-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: gamma gliadin 286-294
Chemical type: Peptide from protein
Sequence: QLEAIRSLV
Position: 286-294
Source molecule (GenBank): P08453.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 17070.0

Epitope: gamma gliadin 286-294
Chemical type: Peptide from protein
Sequence: QLEAIRSLV
Position: 286-294
Source molecule (GenBank): P08453.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 353.0

Epitope: alpha gliadin 217-226
Chemical type: Peptide from protein
Sequence: VSFQQPQQQY
Position: 217-226
Source molecule (GenBank): Q9M4L6.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 365.0

Epitope: alpha gliadin 217-226
Chemical type: Peptide from protein
Sequence: VSFQQPQQQY
Position: 217-226
Source molecule (GenBank): Q9M4L6.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 100000.0

Epitope: omega gliadin 12-20
Chemical type: Peptide from protein
Sequence: MAMKIATAA
Position: 12-20
Source molecule (GenBank): AAG17702.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 100000.0

Epitope: omega gliadin 12-20
Chemical type: Peptide from protein
Sequence: MAMKIATAA
Position: 12-20
Source molecule (GenBank): AAG17702.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 5.2

Epitope: gamma gliadin 159-167
Chemical type: Peptide from protein
Sequence: FLLQQCKPV
Position: 159-167
Source molecule (GenBank): P08079.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 100000.0

Epitope: gamma gliadin 159-167
Chemical type: Peptide from protein
Sequence: FLLQQCKPV
Position: 159-167
Source molecule (GenBank): P08079.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 7.8

Epitope: gamma gliadin 160-169
Chemical type: Peptide from protein
Sequence: LLQQCKPVSL
Position: 160-169
Source molecule (GenBank): P08079.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 100000.0

Epitope: gamma gliadin 160-169
Chemical type: Peptide from protein
Sequence: LLQQCKPVSL
Position: 160-169
Source molecule (GenBank): P08079.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 14.0

Epitope: gamma gliadin 188-197
Chemical type: Peptide from protein
Sequence: LLQQSKPASL
Position: 188-197
Source molecule (GenBank): P08453.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 33513.0

Epitope: gamma gliadin 188-197
Chemical type: Peptide from protein
Sequence: LLQQSKPASL
Position: 188-197
Source molecule (GenBank): P08453.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 25.0

Epitope: gamma gliadin 161-169
Chemical type: Peptide from protein
Sequence: LQQCKPVSL
Position: 161-169
Source molecule (GenBank): P08079.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 100000.0

Epitope: gamma gliadin 161-169
Chemical type: Peptide from protein
Sequence: LQQCKPVSL
Position: 161-169
Source molecule (GenBank): P08079.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 72.0

Epitope: gamma gliadin 165-173
Chemical type: Peptide from protein
Sequence: FPQQQRPFI
Position: 165-173
Source molecule (GenBank): P08453.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 100000.0

Epitope: gamma gliadin 165-173
Chemical type: Peptide from protein
Sequence: FPQQQRPFI
Position: 165-173
Source molecule (GenBank): P08453.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 112.0

Epitope: omega gliadin 10-19
Chemical type: Peptide from protein
Sequence: LAMAMKIATA
Position: 10-19
Source molecule (GenBank): AAG17702.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 100000.0

Epitope: omega gliadin 10-19
Chemical type: Peptide from protein
Sequence: LAMAMKIATA
Position: 10-19
Source molecule (GenBank): AAG17702.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 161.0

Epitope: alpha gliadin 141-149
Chemical type: Peptide from protein
Sequence: LIPCRDVVL
Position: 141-149
Source molecule (GenBank): Q9M4L6.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 100000.0

Epitope: alpha gliadin 141-149
Chemical type: Peptide from protein
Sequence: LIPCRDVVL
Position: 141-149
Source molecule (GenBank): Q9M4L6.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 246.0

Epitope: gamma gliadin 31-40
Chemical type: Peptide from protein
Sequence: WLQQQLVPQL
Position: 31-40
Source molecule (GenBank): P08453.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 100000.0

Epitope: gamma gliadin 31-40
Chemical type: Peptide from protein
Sequence: WLQQQLVPQL
Position: 31-40
Source molecule (GenBank): P08453.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 286.0

Epitope: omega gliadin 14-22
Chemical type: Peptide from protein
Sequence: MNIASASRL
Position: 14-22
Source molecule (GenBank): Q402I5.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 100000.0

Epitope: omega gliadin 14-22
Chemical type: Peptide from protein
Sequence: MNIASASRL
Position: 14-22
Source molecule (GenBank): Q402I5.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 368.0