Reference: Xueqing Li; Ziwei Li; Mulin Ma; Na Yang; Shanyao Du; Ming Liao; Manman Dai. "Revealing novel and conservative CD8<sup>+</sup>T-cell epitopes with MHC B2 restriction on ALV-J." Veterinary research (2024).

Epitope: gag 467-475
Chemical type: Peptide from protein
Sequence: TAPLTDQGI
Position: 467-475
Source molecule (GenBank): Q7SQ98.2
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gag 280-288
Chemical type: Peptide from protein
Sequence: EALMSSPLL
Position: 280-288
Source molecule (GenBank): Q7SQ98.2
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gag 601-609
Chemical type: Peptide from protein
Sequence: PVKQRSVYI
Position: 601-609
Source molecule (GenBank): Q7SQ98.2
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gag 229-237
Chemical type: Peptide from protein
Sequence: LASTGPPVV
Position: 229-237
Source molecule (GenBank): Q7SQ98.2
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gag 475-483
Chemical type: Peptide from protein
Sequence: IAAAMSSAI
Position: 475-483
Source molecule (GenBank): Q7SQ98.2
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gag 460-468
Chemical type: Peptide from protein
Sequence: AALLRPGEL
Source molecule (GenBank): Q7SQ98.2
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gag 633-641
Chemical type: Peptide from protein
Sequence: VVDTANPQI
Position: 633-641
Source molecule (GenBank): Q7SQ98.2
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gag 374-382
Chemical type: Peptide from protein
Sequence: SALQAFREV
Position: 374-382
Source molecule (GenBank): Q7SQ98.2
Assay category: TCell
Result: Positive
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gag 374-382
Chemical type: Peptide from protein
Sequence: SALQAFREV
Position: 374-382
Source molecule (GenBank): Q7SQ98.2
Assay category: TCell
Result: Positive
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gag 374-382
Chemical type: Peptide from protein
Sequence: SALQAFREV
Position: 374-382
Source molecule (GenBank): Q7SQ98.2
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gag 374-382
Chemical type: Peptide from protein
Sequence: SALQAFREV
Position: 374-382
Source molecule (GenBank): Q7SQ98.2
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gag 374-382
Chemical type: Peptide from protein
Sequence: SALQAFREV
Position: 374-382
Source molecule (GenBank): Q7SQ98.2
Assay category: TCell
Result: Positive
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gag 636-644
Chemical type: Peptide from protein
Sequence: TANPQIHGI
Position: 636-644
Source molecule (GenBank): Q7SQ98.2
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gag 677-685
Chemical type: Peptide from protein
Sequence: AVAMVRGSI
Position: 677-685
Source molecule (GenBank): Q7SQ98.2
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gag 406-414
Chemical type: Peptide from protein
Sequence: FANRLIKAV
Position: 406-414
Source molecule (GenBank): Q7SQ98.2
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gag 235-243
Chemical type: Peptide from protein
Sequence: PVVAMPVVI
Position: 235-243
Source molecule (GenBank): Q7SQ98.2
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gag 678-686
Chemical type: Peptide from protein
Sequence: VAMVRGSIL
Position: 678-686
Source molecule (GenBank): Q7SQ98.2
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gag 291-299
Chemical type: Peptide from protein
Sequence: DVTNLMRVI
Position: 291-299
Source molecule (GenBank): Q7SQ98.2
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gag 660-668
Chemical type: Peptide from protein
Sequence: GVINRDGSL
Position: 660-668
Source molecule (GenBank): Q7SQ98.2
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gag 63-71
Chemical type: Peptide from protein
Sequence: MVLGKSGEL
Position: 63-71
Source molecule (GenBank): Q7SQ98.2
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gag 403-411
Chemical type: Peptide from protein
Sequence: FVDFANRLI
Position: 403-411
Source molecule (GenBank): Q7SQ98.2
Assay category: TCell
Result: Positive
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gag 403-411
Chemical type: Peptide from protein
Sequence: FVDFANRLI
Position: 403-411
Source molecule (GenBank): Q7SQ98.2
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gag 403-411
Chemical type: Peptide from protein
Sequence: FVDFANRLI
Position: 403-411
Source molecule (GenBank): Q7SQ98.2
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gag 403-411
Chemical type: Peptide from protein
Sequence: FVDFANRLI
Position: 403-411
Source molecule (GenBank): Q7SQ98.2
Assay category: TCell
Result: Positive
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gag 403-411
Chemical type: Peptide from protein
Sequence: FVDFANRLI
Position: 403-411
Source molecule (GenBank): Q7SQ98.2
Assay category: TCell
Result: Positive
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: pol 652-660
Chemical type: Peptide from protein
Sequence: TVDTASSAI
Position: 652-660
Source molecule (GenBank): AED98689.1
Assay category: TCell
Result: Positive
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: pol 652-660
Chemical type: Peptide from protein
Sequence: TVDTASSAI
Position: 652-660
Source molecule (GenBank): AED98689.1
Assay category: TCell
Result: Positive
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: pol 652-660
Chemical type: Peptide from protein
Sequence: TVDTASSAI
Position: 652-660
Source molecule (GenBank): AED98689.1
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: pol 652-660
Chemical type: Peptide from protein
Sequence: TVDTASSAI
Position: 652-660
Source molecule (GenBank): AED98689.1
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: pol 652-660
Chemical type: Peptide from protein
Sequence: TVDTASSAI
Position: 652-660
Source molecule (GenBank): AED98689.1
Assay category: TCell
Result: Positive
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: pol 852-860
Chemical type: Peptide from protein
Sequence: EASPLFAGI
Position: 852-860
Source molecule (GenBank): AED98689.1
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: pol 376-384
Chemical type: Peptide from protein
Sequence: AVRTFGKEV
Position: 376-384
Source molecule (GenBank): AED98689.1
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: pol 185-193
Chemical type: Peptide from protein
Sequence: LAASSHDGL
Position: 186-194
Source molecule (GenBank): AED98689.1
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: pol 160-168
Chemical type: Peptide from protein
Sequence: VVGQVLEPL
Position: 160-168
Source molecule (GenBank): AED98689.1
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gp85 66-74
Chemical type: Peptide from protein
Sequence: LASQTACLI
Position: 66-74
Source molecule (GenBank): ADM72957.1
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gp85 249-257
Chemical type: Peptide from protein
Sequence: TAKALPPGI
Position: 249-257
Source molecule (GenBank): ADM72957.1
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gp85 251-259
Chemical type: Peptide from protein
Sequence: KALPPGIFL
Position: 251-259
Source molecule (GenBank): ADM72957.1
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gp37 33-40
Chemical type: Peptide from protein
Sequence: AAAQALREI
Position: 429-437
Source molecule (GenBank): AKP18449.1
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gp37 71-79
Chemical type: Peptide from protein
Sequence: AVLQNRAAI
Position: 468-476
Source molecule (GenBank): AKP18449.1
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gp37 76-84
Chemical type: Peptide from protein
Sequence: RAAIDFLLL
Position: 473-481
Source molecule (GenBank): AKP18449.1
Assay category: TCell
Result: Negative
MHC allele types: 20433
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo