Reference: Anouk C M Platteel; Juliane Liepe; Kathrin Textoris-Taube; Christin Keller; Petra Henklein; Hanna H Schalkwijk; Rebeca Cardoso; Peter M Kloetzel; Michele Mishto; Alice J A M Sijts. "Multi-level Strategy for Identifying Proteasome-Catalyzed Spliced Epitopes Targeted by CD8<sup>+</sup> T Cells during Bacterial Infection." Cell reports (2017).

Epitope: LLO 296-304
Chemical type: Peptide from protein
Sequence: VAYGRQVYL
Position: 296-304
Source molecule (GenBank): P13128.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: LLO 296-304
Chemical type: Peptide from protein
Sequence: VAYGRQVYL
Position: 296-304
Source molecule (GenBank): P13128.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 84.0

Epitope: PlcB 189-191/163-167
Chemical type: Peptide, no natural source
Sequence: ISYAFYKL
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcB 189-191/163-167
Chemical type: Peptide, no natural source
Sequence: ISYAFYKL
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 315.0

Epitope: PlcB 189-192/164-167
Chemical type: Peptide, no natural source
Sequence: ISYPFYKL
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcB 189-192/164-167
Chemical type: Peptide, no natural source
Sequence: ISYPFYKL
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 243.0

Epitope: PlcA 70–72/92–96
Chemical type: Peptide, no natural source
Sequence: MSYLYQQL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcA 70–72/92–96
Chemical type: Peptide, no natural source
Sequence: MSYLYQQL
Assay category: MhcBinding
Result: Negative

Epitope: PlcA 70–73/93–96
Chemical type: Peptide, no natural source
Sequence: MSYNYQQL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcA 70–73/93–96
Chemical type: Peptide, no natural source
Sequence: MSYNYQQL
Assay category: MhcBinding
Result: Negative

Epitope: PlcA 262–266/273–275
Chemical type: Peptide, no natural source
Sequence: TSLTFAAL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcA 262–266/273–275
Chemical type: Peptide, no natural source
Sequence: TSLTFAAL
Assay category: MhcBinding
Result: Negative

Epitope: PlcA  262–267/285–286
Chemical type: Peptide, no natural source
Sequence: TSLTFTNL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcA  262–267/285–286
Chemical type: Peptide, no natural source
Sequence: TSLTFTNL
Assay category: MhcBinding
Result: Negative

Epitope: PlcA 262–268/254
Chemical type: Peptide, no natural source
Sequence: TSLTFTPL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcA 262–268/254
Chemical type: Peptide, no natural source
Sequence: TSLTFTPL
Assay category: MhcBinding
Result: Negative

Epitope: PlcA 70–72/57–61
Chemical type: Peptide, no natural source
Sequence: MSYNLAAL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcA 70–72/57–61
Chemical type: Peptide, no natural source
Sequence: MSYNLAAL
Assay category: MhcBinding
Result: Negative

Epitope: PlcA 262–266/284–286
Chemical type: Peptide, no natural source
Sequence: TSLTFLNL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcA 262–266/284–286
Chemical type: Peptide, no natural source
Sequence: TSLTFLNL
Assay category: MhcBinding
Result: Negative

Epitope: PlcA 112–114/92–96
Chemical type: Peptide, no natural source
Sequence: KIYLYQQL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcA 112–114/92–96
Chemical type: Peptide, no natural source
Sequence: KIYLYQQL
Assay category: MhcBinding
Result: Negative

Epitope: PlcA 19–23/59–61
Chemical type: Peptide, no natural source
Sequence: CFFTFAAL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcA 19–23/59–61
Chemical type: Peptide, no natural source
Sequence: CFFTFAAL
Assay category: MhcBinding
Result: Negative

Epitope: PlcA 19–23/56–58
Chemical type: Peptide, no natural source
Sequence: CFFTFTNL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcA 19–23/56–58
Chemical type: Peptide, no natural source
Sequence: CFFTFTNL
Assay category: MhcBinding
Result: Negative

Epitope: PlcA 19–23/50–52
Chemical type: Peptide, no natural source
Sequence: CFFTFSAL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcA 19–23/50–52
Chemical type: Peptide, no natural source
Sequence: CFFTFSAL
Assay category: MhcBinding
Result: Negative

Epitope: PlcA 19–24/57-58
Chemical type: Peptide, no natural source
Sequence: CFFTFPNL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcA 19–24/57-58
Chemical type: Peptide, no natural source
Sequence: CFFTFPNL
Assay category: MhcBinding
Result: Negative

Epitope: PlcB 171–173/163–167
Chemical type: Peptide, no natural source
Sequence: IHYAFYKL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcB 171–173/163–167
Chemical type: Peptide, no natural source
Sequence: IHYAFYKL
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 143.4

Epitope: PlcB 65–69/88–90
Chemical type: Peptide, no natural source
Sequence: VNTHYANL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcB 65–69/88–90
Chemical type: Peptide, no natural source
Sequence: VNTHYANL
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 286.8

Epitope: PlcB 189–191/181–185
Chemical type: Peptide, no natural source
Sequence: ISYMHANN
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcB 189–191/181–185
Chemical type: Peptide, no natural source
Sequence: ISYMHANN
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 10.2

Epitope: PlcB 171–174/164–167
Chemical type: Peptide, no natural source
Sequence: IHYYFYKL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcB 171–174/164–167
Chemical type: Peptide, no natural source
Sequence: IHYYFYKL
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 99.6

Epitope: PlcB 251–255/270–272
Chemical type: Peptide, no natural source
Sequence: KSYLVARL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcB 251–255/270–272
Chemical type: Peptide, no natural source
Sequence: KSYLVARL
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 187.8

Epitope: PlcB 118–121/137–140
Chemical type: Peptide, no natural source
Sequence: STFLFANA
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcB 118–121/137–140
Chemical type: Peptide, no natural source
Sequence: STFLFANA
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 92.4

Epitope: PlcB 189–192/182–185
Chemical type: Peptide, no natural source
Sequence: ISYPHANN
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcB 189–192/182–185
Chemical type: Peptide, no natural source
Sequence: ISYPHANN
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 13.2

Epitope: PlcB 177–179/163–167
Chemical type: Peptide, no natural source
Sequence: ISQAFYKL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcB 177–179/163–167
Chemical type: Peptide, no natural source
Sequence: ISQAFYKL
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 85.8

Epitope: PlcB 160–167
Chemical type: Peptide from protein
Sequence: FDTAFYKL
Position: 157-164
Source molecule (GenBank): AAS85301.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcB 160–167
Chemical type: Peptide from protein
Sequence: FDTAFYKL
Position: 157-164
Source molecule (GenBank): AAS85301.1
Assay category: MhcBinding
Result: Negative

Epitope: PlcB 189–196
Chemical type: Peptide from protein
Sequence: ISYPPGYH
Position: 186-193
Source molecule (GenBank): AAS85301.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcB 189–196
Chemical type: Peptide from protein
Sequence: ISYPPGYH
Position: 186-193
Source molecule (GenBank): AAS85301.1
Assay category: MhcBinding
Result: Negative

Epitope: PlcB 189–191/163,164S–167S
Chemical type: Peptide, no natural source
Sequence: ISYASYKS
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcB 189–191/163,164S–167S
Chemical type: Peptide, no natural source
Sequence: ISYASYKS
Assay category: MhcBinding
Result: Negative

Epitope: PlcB 189–192/164S–167S
Chemical type: Peptide, no natural source
Sequence: ISYPSYKS
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PlcB 189–192/164S–167S
Chemical type: Peptide, no natural source
Sequence: ISYPSYKS
Assay category: MhcBinding
Result: Negative