Reference: David C Tscharke; Gunasegaran Karupiah; Jie Zhou; Tara Palmore; Kari R Irvine; S M Mansour Haeryfar; Shanicka Williams; John Sidney; Alessandro Sette; Jack R Bennink; Jonathan W Yewdell. "Identification of poxvirus CD8+ T cell determinants to enable rational design and characterization of smallpox vaccines." The Journal of experimental medicine (2005).

Epitope: B8 52-60
Chemical type: Peptide from protein
Sequence: WQTMYTNV
Position: 53-60
Source molecule (GenBank): P24770.1
Assay category: TCell
Result: Negative
MHC allele types: 122
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B8 52-60
Chemical type: Peptide from protein
Sequence: WQTMYTNV
Position: 53-60
Source molecule (GenBank): P24770.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 6.6

Epitope: B8 42-50
Chemical type: Peptide from protein
Sequence: ISLKNYGI
Position: 43-50
Source molecule (GenBank): P24770.1
Assay category: TCell
Result: Negative
MHC allele types: 122
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B8 42-50
Chemical type: Peptide from protein
Sequence: ISLKNYGI
Position: 43-50
Source molecule (GenBank): P24770.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 16.0

Epitope: B8 7-15
Chemical type: Peptide from protein
Sequence: LAVLFINSI
Position: 7-15
Source molecule (GenBank): P24770.1
Assay category: TCell
Result: Negative
MHC allele types: 122
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B8 7-15
Chemical type: Peptide from protein
Sequence: LAVLFINSI
Position: 7-15
Source molecule (GenBank): P24770.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 28.0

Epitope: B8 88-96
Chemical type: Peptide from protein
Sequence: KVEEYCTGL
Position: 88-96
Source molecule (GenBank): P24770.1
Assay category: TCell
Result: Negative
MHC allele types: 122
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B8 88-96
Chemical type: Peptide from protein
Sequence: KVEEYCTGL
Position: 88-96
Source molecule (GenBank): P24770.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 129.0

Epitope: A19 42-49
Chemical type: Peptide from protein
Sequence: SDITKVSL
Position: 42-49
Source molecule (GenBank): P20994.1
Assay category: TCell
Result: Negative
MHC allele types: 122
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A19 42-49
Chemical type: Peptide from protein
Sequence: SDITKVSL
Position: 42-49
Source molecule (GenBank): P20994.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 1261.0

Epitope: A19 39-47
Chemical type: Peptide from protein
Sequence: VKISDITKV
Position: 39-47
Source molecule (GenBank): P20994.1
Assay category: TCell
Result: Negative
MHC allele types: 122
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A19 39-47
Chemical type: Peptide from protein
Sequence: VKISDITKV
Position: 39-47
Source molecule (GenBank): P20994.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 4930.0

Epitope: A47 42-49
Chemical type: Peptide from protein
Sequence: TIQRFSSL
Position: 42-49
Source molecule (GenBank): P26673.2
Assay category: TCell
Result: Negative
MHC allele types: 122
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A47 42-49
Chemical type: Peptide from protein
Sequence: TIQRFSSL
Position: 42-49
Source molecule (GenBank): P26673.2
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.1

Epitope: A47 41-49
Chemical type: Peptide from protein
Sequence: ATIQRFSSL
Position: 41-49
Source molecule (GenBank): P26673.2
Assay category: TCell
Result: Negative
MHC allele types: 122
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A47 41-49
Chemical type: Peptide from protein
Sequence: ATIQRFSSL
Position: 41-49
Source molecule (GenBank): P26673.2
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.3

Epitope: A47 228-236
Chemical type: Peptide from protein
Sequence: VSDLYTSM
Position: 229-236
Source molecule (GenBank): P26673.2
Assay category: TCell
Result: Negative
MHC allele types: 122
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A47 228-236
Chemical type: Peptide from protein
Sequence: VSDLYTSM
Position: 229-236
Source molecule (GenBank): P26673.2
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 7.0

Epitope: A42 40-48
Chemical type: Peptide from protein
Sequence: FAKINPGEI
Position: 40-48
Source molecule (GenBank): Q76ZN5.1
Assay category: TCell
Result: Negative
MHC allele types: 122
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A42 40-48
Chemical type: Peptide from protein
Sequence: FAKINPGEI
Position: 40-48
Source molecule (GenBank): Q76ZN5.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 54.0

Epitope: A42 74-82
Chemical type: Peptide from protein
Sequence: LMDENTYAM
Position: 74-82
Source molecule (GenBank): Q76ZN5.1
Assay category: TCell
Result: Negative
MHC allele types: 122
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A42 74-82
Chemical type: Peptide from protein
Sequence: LMDENTYAM
Position: 74-82
Source molecule (GenBank): Q76ZN5.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 767.0

Epitope: A42 87-96
Chemical type: Peptide from protein
Sequence: MYAPVSPIVI
Assay category: TCell
Result: Negative
MHC allele types: 122
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A42 87-96
Chemical type: Peptide from protein
Sequence: MYAPVSPIVI
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 1266.0

Epitope: A42 40-49
Chemical type: Peptide from protein
Sequence: FAKINPGEII
Position: 40-49
Source molecule (GenBank): Q76ZN5.1
Assay category: TCell
Result: Negative
MHC allele types: 122
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A42 40-49
Chemical type: Peptide from protein
Sequence: FAKINPGEII
Position: 40-49
Source molecule (GenBank): Q76ZN5.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 2071.0

Epitope: K3 76-84
Chemical type: Peptide from protein
Sequence: YIDVNYKRM
Position: 76-84
Source molecule (GenBank): P18378.1
Assay category: TCell
Result: Negative
MHC allele types: 122
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: K3 76-84
Chemical type: Peptide from protein
Sequence: YIDVNYKRM
Position: 76-84
Source molecule (GenBank): P18378.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 5138.0

Epitope: B8R 20-27
Chemical type: Peptide from protein
Sequence: TSYKFESV
Position: 20-27
Source molecule (GenBank): P24770.1
Assay category: TCell
Result: Positive-High
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B8R 20-27
Chemical type: Peptide from protein
Sequence: TSYKFESV
Position: 20-27
Source molecule (GenBank): P24770.1
Assay category: TCell
Result: Positive

Epitope: B8R 20-27
Chemical type: Peptide from protein
Sequence: TSYKFESV
Position: 20-27
Source molecule (GenBank): P24770.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B8R 20-27
Chemical type: Peptide from protein
Sequence: TSYKFESV
Position: 20-27
Source molecule (GenBank): P24770.1
Assay category: TCell
Result: Positive-High
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B8R 20-27
Chemical type: Peptide from protein
Sequence: TSYKFESV
Position: 20-27
Source molecule (GenBank): P24770.1
Assay category: TCell
Result: Positive-High
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B8R 20-27
Chemical type: Peptide from protein
Sequence: TSYKFESV
Position: 20-27
Source molecule (GenBank): P24770.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 0.19

Epitope: A19L 47-55
Chemical type: Peptide from protein
Sequence: VSLDYINTM
Position: 47-55
Source molecule (GenBank): P20994.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A19L 47-55
Chemical type: Peptide from protein
Sequence: VSLDYINTM
Position: 47-55
Source molecule (GenBank): P20994.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A19L 47-55
Chemical type: Peptide from protein
Sequence: VSLDYINTM
Position: 47-55
Source molecule (GenBank): P20994.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A19L 47-55
Chemical type: Peptide from protein
Sequence: VSLDYINTM
Position: 47-55
Source molecule (GenBank): P20994.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A19L 47-55
Chemical type: Peptide from protein
Sequence: VSLDYINTM
Position: 47-55
Source molecule (GenBank): P20994.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A19L 47-55
Chemical type: Peptide from protein
Sequence: VSLDYINTM
Position: 47-55
Source molecule (GenBank): P20994.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 0.77

Epitope: A47L 138-146
Chemical type: Peptide from protein
Sequence: AAFEFINSL
Position: 138-146
Source molecule (GenBank): P26673.2
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A47L 138-146
Chemical type: Peptide from protein
Sequence: AAFEFINSL
Position: 138-146
Source molecule (GenBank): P26673.2
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A47L 138-146
Chemical type: Peptide from protein
Sequence: AAFEFINSL
Position: 138-146
Source molecule (GenBank): P26673.2
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A47L 138-146
Chemical type: Peptide from protein
Sequence: AAFEFINSL
Position: 138-146
Source molecule (GenBank): P26673.2
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A47L 138-146
Chemical type: Peptide from protein
Sequence: AAFEFINSL
Position: 138-146
Source molecule (GenBank): P26673.2
Assay category: TCell
Result: Positive-Intermediate
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A47L 138-146
Chemical type: Peptide from protein
Sequence: AAFEFINSL
Position: 138-146
Source molecule (GenBank): P26673.2
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.3

Epitope: A42R 88-96
Chemical type: Peptide from protein
Sequence: YAPVSPIVI
Position: 88-96
Source molecule (GenBank): Q76ZN5.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A42R 88-96
Chemical type: Peptide from protein
Sequence: YAPVSPIVI
Position: 88-96
Source molecule (GenBank): Q76ZN5.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A42R 88-96
Chemical type: Peptide from protein
Sequence: YAPVSPIVI
Position: 88-96
Source molecule (GenBank): Q76ZN5.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A42R 88-96
Chemical type: Peptide from protein
Sequence: YAPVSPIVI
Position: 88-96
Source molecule (GenBank): Q76ZN5.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A42R 88-96
Chemical type: Peptide from protein
Sequence: YAPVSPIVI
Position: 88-96
Source molecule (GenBank): Q76ZN5.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A42R 88-96
Chemical type: Peptide from protein
Sequence: YAPVSPIVI
Position: 88-96
Source molecule (GenBank): Q76ZN5.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A42R 88-96
Chemical type: Peptide from protein
Sequence: YAPVSPIVI
Position: 88-96
Source molecule (GenBank): Q76ZN5.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 0.24

Epitope: K3L 6-15
Chemical type: Peptide from protein
Sequence: YSLPNAGDVI
Position: 6-15
Source molecule (GenBank): P18378.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: K3L 6-15
Chemical type: Peptide from protein
Sequence: YSLPNAGDVI
Position: 6-15
Source molecule (GenBank): P18378.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: K3L 6-15
Chemical type: Peptide from protein
Sequence: YSLPNAGDVI
Position: 6-15
Source molecule (GenBank): P18378.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: K3L 6-15
Chemical type: Peptide from protein
Sequence: YSLPNAGDVI
Position: 6-15
Source molecule (GenBank): P18378.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: K3L 6-15
Chemical type: Peptide from protein
Sequence: YSLPNAGDVI
Position: 6-15
Source molecule (GenBank): P18378.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 0.22