Reference: Arunan Kaliyaperumal; Marissa A Michaels; Syamal K Datta. "Naturally processed chromatin peptides reveal a major autoepitope that primes pathogenic T and B cells of lupus." Journal of immunology (Baltimore, Md. : 1950) (2002).

Epitope: EP-1
Chemical type: Peptide from protein
Sequence: SQKEEEEGAQREKE
Assay category: TCell
Result: Negative
Host: F 12 weeks

Epitope: EP-1
Chemical type: Peptide from protein
Sequence: SQKEEEEGAQREKE
Assay category: TCell
Result: Positive-Low
Host:  12 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: EP-1
Chemical type: Peptide from protein
Sequence: SQKEEEEGAQREKE
Assay category: TCell
Result: Negative
Host:  12 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: EP-1
Chemical type: Peptide from protein
Sequence: SQKEEEEGAQREKE
Assay category: TCell
Result: Negative
Host:  12 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: EP-1
Chemical type: Peptide from protein
Sequence: SQKEEEEGAQREKE
Assay category: MhcLigandElution
Result: Positive

Epitope: EP-2
Chemical type: Peptide from protein
Sequence: DWMEEEEGAQREKE
Assay category: TCell
Result: Positive-Low
Host: F 12 weeks

Epitope: EP-2
Chemical type: Peptide from protein
Sequence: DWMEEEEGAQREKE
Assay category: TCell
Result: Positive-Intermediate
Host:  12 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: EP-2
Chemical type: Peptide from protein
Sequence: DWMEEEEGAQREKE
Assay category: TCell
Result: Negative
Host:  12 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: EP-2
Chemical type: Peptide from protein
Sequence: DWMEEEEGAQREKE
Assay category: TCell
Result: Negative
Host:  12 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: EP-2
Chemical type: Peptide from protein
Sequence: DWMEEEEGAQREKE
Assay category: MhcLigandElution
Result: Positive

Epitope: EP-3 (Chicken H5<sub>22-42</sub>)
Chemical type: Peptide from protein
Sequence: SASHPTYSEMIAAAIRAEKSR
Position: 23-43
Source molecule (GenBank): NP_001038138.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: EP-3 (Chicken H5<sub>22-42</sub>)
Chemical type: Peptide from protein
Sequence: SASHPTYSEMIAAAIRAEKSR
Position: 23-43
Source molecule (GenBank): NP_001038138.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Mouse H1'<sub>22-42</sub>
Chemical type: Peptide from protein
Sequence: STDHPKYSDMIVAAIQAEKNR
Position: 22-42
Source molecule (GenBank): NP_032223.2
Assay category: BCell
Result: Negative

Epitope: Mouse H1'<sub>22-42</sub>
Chemical type: Peptide from protein
Sequence: STDHPKYSDMIVAAIQAEKNR
Position: 22-42
Source molecule (GenBank): NP_032223.2
Assay category: BCell
Result: Positive

Epitope: Mouse H1'<sub>22-42</sub>
Chemical type: Peptide from protein
Sequence: STDHPKYSDMIVAAIQAEKNR
Position: 22-42
Source molecule (GenBank): NP_032223.2
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Mouse H1'<sub>22-42</sub>
Chemical type: Peptide from protein
Sequence: STDHPKYSDMIVAAIQAEKNR
Position: 22-42
Source molecule (GenBank): NP_032223.2
Assay category: TCell
Result: Negative
Host:  12 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Mouse H1'<sub>22-42</sub>
Chemical type: Peptide from protein
Sequence: STDHPKYSDMIVAAIQAEKNR
Position: 22-42
Source molecule (GenBank): NP_032223.2
Assay category: TCell
Result: Negative
Host:  12 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Mouse H1'<sub>22-42</sub>
Chemical type: Peptide from protein
Sequence: STDHPKYSDMIVAAIQAEKNR
Position: 22-42
Source molecule (GenBank): NP_032223.2
Assay category: TCell
Result: Positive

Epitope: Mouse H1'<sub>22-42</sub>
Chemical type: Peptide from protein
Sequence: STDHPKYSDMIVAAIQAEKNR
Position: 22-42
Source molecule (GenBank): NP_032223.2
Assay category: TCell
Result: Positive-High
Host:  12 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Mouse H1'<sub>22-42</sub>
Chemical type: Peptide from protein
Sequence: STDHPKYSDMIVAAIQAEKNR
Position: 22-42
Source molecule (GenBank): NP_032223.2
Assay category: TCell
Result: Positive-High
Host: F 12 weeks

Epitope: Mouse H1'<sub>22-42</sub>
Chemical type: Peptide from protein
Sequence: STDHPKYSDMIVAAIQAEKNR
Position: 22-42
Source molecule (GenBank): NP_032223.2
Assay category: TCell
Result: Positive
Host:  4 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo