Reference: L Brandt; T Oettinger; A Holm; A B Andersen; P Andersen. "Key epitopes on the ESAT-6 antigen recognized in mice during the recall of protective immunity to Mycobacterium tuberculosis." Journal of immunology (Baltimore, Md. : 1950) (1996).

Epitope: p1
Chemical type: Peptide from protein
Sequence: MTEQQWNFAGIEAAASAIQG
Position: 1-20
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p1
Chemical type: Peptide from protein
Sequence: MTEQQWNFAGIEAAASAIQG
Position: 1-20
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p2
Chemical type: Peptide from protein
Sequence: IEAAASAIQGNVTSIHSLLD
Position: 11-30
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p2
Chemical type: Peptide from protein
Sequence: IEAAASAIQGNVTSIHSLLD
Position: 11-30
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p2
Chemical type: Peptide from protein
Sequence: IEAAASAIQGNVTSIHSLLD
Position: 11-30
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p2
Chemical type: Peptide from protein
Sequence: IEAAASAIQGNVTSIHSLLD
Position: 11-30
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p2
Chemical type: Peptide from protein
Sequence: IEAAASAIQGNVTSIHSLLD
Position: 11-30
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p3
Chemical type: Peptide from protein
Sequence: NVTSIHSLLDEGKQSLTKLA
Position: 21-40
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p3
Chemical type: Peptide from protein
Sequence: NVTSIHSLLDEGKQSLTKLA
Position: 21-40
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p3
Chemical type: Peptide from protein
Sequence: NVTSIHSLLDEGKQSLTKLA
Position: 21-40
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p3
Chemical type: Peptide from protein
Sequence: NVTSIHSLLDEGKQSLTKLA
Position: 21-40
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p3
Chemical type: Peptide from protein
Sequence: NVTSIHSLLDEGKQSLTKLA
Position: 21-40
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p4
Chemical type: Peptide from protein
Sequence: EGKQSLTKLAAAWGGSGSEA
Position: 31-50
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p4
Chemical type: Peptide from protein
Sequence: EGKQSLTKLAAAWGGSGSEA
Position: 31-50
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p4
Chemical type: Peptide from protein
Sequence: EGKQSLTKLAAAWGGSGSEA
Position: 31-50
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p4
Chemical type: Peptide from protein
Sequence: EGKQSLTKLAAAWGGSGSEA
Position: 31-50
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Positive-Low
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p4
Chemical type: Peptide from protein
Sequence: EGKQSLTKLAAAWGGSGSEA
Position: 31-50
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p5
Chemical type: Peptide from protein
Sequence: AAWGGSGSEAYQGVQQKWDA
Position: 41-60
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p5
Chemical type: Peptide from protein
Sequence: AAWGGSGSEAYQGVQQKWDA
Position: 41-60
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p5
Chemical type: Peptide from protein
Sequence: AAWGGSGSEAYQGVQQKWDA
Position: 41-60
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p5
Chemical type: Peptide from protein
Sequence: AAWGGSGSEAYQGVQQKWDA
Position: 41-60
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p5
Chemical type: Peptide from protein
Sequence: AAWGGSGSEAYQGVQQKWDA
Position: 41-60
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p6
Chemical type: Peptide from protein
Sequence: YQGVQQKWDATATELNNALQ
Position: 51-70
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p6
Chemical type: Peptide from protein
Sequence: YQGVQQKWDATATELNNALQ
Position: 51-70
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p8
Chemical type: Peptide from protein
Sequence: NLARTISEAGQAMASTEGNV
Position: 71-90
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p8
Chemical type: Peptide from protein
Sequence: NLARTISEAGQAMASTEGNV
Position: 71-90
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p8
Chemical type: Peptide from protein
Sequence: NLARTISEAGQAMASTEGNV
Position: 71-90
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p8
Chemical type: Peptide from protein
Sequence: NLARTISEAGQAMASTEGNV
Position: 71-90
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p8
Chemical type: Peptide from protein
Sequence: NLARTISEAGQAMASTEGNV
Position: 71-90
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p9
Chemical type: Peptide from protein
Sequence: QAMASTEGNVTGMFA
Position: 81-95
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p9
Chemical type: Peptide from protein
Sequence: QAMASTEGNVTGMFA
Position: 81-95
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p9
Chemical type: Peptide from protein
Sequence: QAMASTEGNVTGMFA
Position: 81-95
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p9
Chemical type: Peptide from protein
Sequence: QAMASTEGNVTGMFA
Position: 81-95
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p9
Chemical type: Peptide from protein
Sequence: QAMASTEGNVTGMFA
Position: 81-95
Source molecule (GenBank): P0A564.2
Assay category: TCell
Result: Positive-Low
Host:  8-12 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo