Reference: Sarah Hulin-Curtis; James K Geary; Bruce J MacLachlan; Danny M Altmann; Laury Baillon; David K Cole; Alex Greenshields-Watson; Sophie J Hesketh; Ian R Humphreys; Ian M Jones; Sarah N Lauder; Georgina H Mason; Kathryn Smart; D Oliver Scourfield; Jake Scott; Ksenia Sukhova; Richard J Stanton; Aaron Wall; Pierre J Rizkallah; Wendy S Barclay; Awen Gallimore; Andrew Godkin. "A targeted single mutation in influenza A virus universal epitope transforms immunogenicity and protective immunity via CD4<sup>+</sup> T cell activation." Cell reports (2024).

Epitope: SGP M1 17-31
Chemical type: Peptide from protein
Sequence: SGPLKAEIAQR
Position: 17-27
Source molecule (GenBank): Q9IQ48.1
Assay category: TCell
Result: Negative
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: SGP M1 17-31
Chemical type: Peptide from protein
Sequence: SGPLKAEIAQR
Position: 17-27
Source molecule (GenBank): Q9IQ48.1
Assay category: TCell
Result: Negative
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: SGP M1 17-31
Chemical type: Peptide from protein
Sequence: SGPLKAEIAQR
Position: 17-27
Source molecule (GenBank): Q9IQ48.1
Assay category: TCell
Result: Negative
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GLI M1 129-143
Chemical type: Peptide from protein
Sequence: GLIYNRMGAVTTEVA
Position: 129-143
Source molecule (GenBank): Q9IQ48.1
Assay category: TCell
Result: Positive
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GLI M1 129-143
Chemical type: Peptide from protein
Sequence: GLIYNRMGAVTTEVA
Position: 129-143
Source molecule (GenBank): Q9IQ48.1
Assay category: TCell
Result: Positive
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GLI M1 129-143
Chemical type: Peptide from protein
Sequence: GLIYNRMGAVTTEVA
Position: 129-143
Source molecule (GenBank): Q9IQ48.1
Assay category: TCell
Result: Positive-Low
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: QAR M1  210-224
Chemical type: Peptide from protein
Sequence: RQMVQAMITIGTHPS
Position: 210-224
Source molecule (GenBank): Q9IQ48.1
Assay category: TCell
Result: Negative
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: QAR M1  210-224
Chemical type: Peptide from protein
Sequence: RQMVQAMITIGTHPS
Position: 210-224
Source molecule (GenBank): Q9IQ48.1
Assay category: TCell
Result: Positive-Low
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: QAR M1  210-224
Chemical type: Peptide from protein
Sequence: RQMVQAMITIGTHPS
Position: 210-224
Source molecule (GenBank): Q9IQ48.1
Assay category: TCell
Result: Negative
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NAE NP 254-268
Chemical type: Peptide from protein
Sequence: EDLIFLARSALILRG
Position: 254-268
Source molecule (GenBank): P22435.2
Assay category: TCell
Result: Negative
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NAE NP 254-268
Chemical type: Peptide from protein
Sequence: EDLIFLARSALILRG
Position: 254-268
Source molecule (GenBank): P22435.2
Assay category: TCell
Result: Positive-Low
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NAE NP 254-268
Chemical type: Peptide from protein
Sequence: EDLIFLARSALILRG
Position: 254-268
Source molecule (GenBank): P22435.2
Assay category: TCell
Result: Positive-Low
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: DPF NP 301-315
Chemical type: Peptide from protein
Sequence: IDPFRLLQNSQVFSL
Position: 301-315
Source molecule (GenBank): AAR12361.1
Assay category: TCell
Result: Positive-Low
Host:  29-39 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: DPF NP 301-315
Chemical type: Peptide from protein
Sequence: IDPFRLLQNSQVFSL
Position: 301-315
Source molecule (GenBank): AAR12361.1
Assay category: TCell
Result: Positive
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: DPF NP 301-315
Chemical type: Peptide from protein
Sequence: IDPFRLLQNSQVFSL
Position: 301-315
Source molecule (GenBank): AAR12361.1
Assay category: TCell
Result: Positive
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: DPF NP 301-315
Chemical type: Peptide from protein
Sequence: IDPFRLLQNSQVFSL
Position: 301-315
Source molecule (GenBank): AAR12361.1
Assay category: TCell
Result: Negative
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NPR PB1 315-329
Chemical type: Peptide from protein
Sequence: PRMFLAMITYMTRNQ
Position: 315-329
Source molecule (GenBank): Q9IQ46.1
Assay category: TCell
Result: Positive
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NPR PB1 315-329
Chemical type: Peptide from protein
Sequence: PRMFLAMITYMTRNQ
Position: 315-329
Source molecule (GenBank): Q9IQ46.1
Assay category: TCell
Result: Positive
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NPR PB1 315-329
Chemical type: Peptide from protein
Sequence: PRMFLAMITYMTRNQ
Position: 315-329
Source molecule (GenBank): Q9IQ46.1
Assay category: TCell
Result: Positive
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GMF PB1 409-423
Chemical type: Peptide from protein
Sequence: MGMFNMLSTVLGVSI
Position: 409-423
Source molecule (GenBank): Q9IQ46.1
Assay category: TCell
Result: Negative
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GMF PB1 409-423
Chemical type: Peptide from protein
Sequence: MGMFNMLSTVLGVSI
Position: 409-423
Source molecule (GenBank): Q9IQ46.1
Assay category: TCell
Result: Negative
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GMF PB1 409-423
Chemical type: Peptide from protein
Sequence: MGMFNMLSTVLGVSI
Position: 409-423
Source molecule (GenBank): Q9IQ46.1
Assay category: TCell
Result: Negative
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CYP HA 118-132
Chemical type: Peptide from protein
Sequence: VPDYASLRSLVASSG
Position: 118-132
Source molecule (GenBank): P03438.2
Assay category: TCell
Result: Negative
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CYP HA 118-132
Chemical type: Peptide from protein
Sequence: VPDYASLRSLVASSG
Position: 118-132
Source molecule (GenBank): P03438.2
Assay category: TCell
Result: Positive-Low
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CYP HA 118-132
Chemical type: Peptide from protein
Sequence: VPDYASLRSLVASSG
Position: 118-132
Source molecule (GenBank): P03438.2
Assay category: TCell
Result: Negative
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PKY HA 320-335
Chemical type: Peptide from protein
Sequence: PKYVKQNTLKLATG
Position: 322-335
Source molecule (GenBank): P03438.2
Assay category: TCell
Result: Negative
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PKY HA 320-335
Chemical type: Peptide from protein
Sequence: PKYVKQNTLKLATG
Position: 322-335
Source molecule (GenBank): P03438.2
Assay category: TCell
Result: Positive
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PKY HA 320-335
Chemical type: Peptide from protein
Sequence: PKYVKQNTLKLATG
Position: 322-335
Source molecule (GenBank): P03438.2
Assay category: TCell
Result: Negative
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PKY HA 320-335
Chemical type: Peptide from protein
Sequence: PKYVKQNTLKLATG
Position: 322-335
Source molecule (GenBank): P03438.2
Assay category: TCell
Result: Positive-Low
Host:  29-39 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: DPF NP 301-315 S11R
Chemical type: Peptide, no natural source
Sequence: IDPFRLLQNSQVFRL
Assay category: TCell
Result: Positive-High
Host:  29-39 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: DPF NP 301-315 S11R
Chemical type: Peptide, no natural source
Sequence: IDPFRLLQNSQVFRL
Assay category: TCell
Result: Positive
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NPR PB1 315-329 N11R
Chemical type: Peptide, no natural source
Sequence: PRMFLAMITYMTRRQ
Assay category: TCell
Result: Positive
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CYP HA 118-132 S11R
Chemical type: Peptide, no natural source
Sequence: VPDYASLRSLVASRG
Assay category: TCell
Result: Positive
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PKY HA 320-335 T11R
Chemical type: Peptide, no natural source
Sequence: PKYVKQNTLKLARG
Assay category: TCell
Result: Positive
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PKY HA 320-335 T11R
Chemical type: Peptide, no natural source
Sequence: PKYVKQNTLKLARG
Assay category: TCell
Result: Positive-High
Host:  29-39 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NAE NP 254-268 R11A
Chemical type: Peptide, no natural source
Sequence: EDLIFLARSALILAG
Assay category: TCell
Result: Negative
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 366-374
Chemical type: Peptide from protein
Sequence: ASNENMETM
Position: 366-374
Source molecule (GenBank): Q91UL1.1
Assay category: TCell
Result: Positive
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 366-374
Chemical type: Peptide from protein
Sequence: ASNENMETM
Position: 366-374
Source molecule (GenBank): Q91UL1.1
Assay category: TCell
Result: Positive
Host:  29-39 weeks
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: PA 224-233
Chemical type: Peptide from protein
Sequence: SSLENFRAYV
Position: 224-233
Source molecule (GenBank): Q9IQ47.1
Assay category: TCell
Result: Positive
Host:  29-39 weeks
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: PA 224-233
Chemical type: Peptide from protein
Sequence: SSLENFRAYV
Position: 224-233
Source molecule (GenBank): Q9IQ47.1
Assay category: TCell
Result: Positive
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PB1-F2 62-70
Chemical type: Peptide from protein
Sequence: LSLRNPILV
Position: 62-70
Source molecule (GenBank): P0C0U1.1
Assay category: TCell
Result: Positive
Host:  29-39 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PB1-F2 62-70
Chemical type: Peptide from protein
Sequence: LSLRNPILV
Position: 62-70
Source molecule (GenBank): P0C0U1.1
Assay category: TCell
Result: Positive
Host:  29-39 weeks
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: PKY
Chemical type: Peptide from protein
Sequence: PKYVKQNTLKLAT
Position: 322-334
Source molecule (GenBank): P03438.2
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 1.7

Epitope: PKY-11R
Chemical type: Peptide, no natural source
Sequence: PKYVKQNTLKLAR
Assay category: TCell
Result: Positive
Quantitative measurement: 1.83

Epitope: PKY-11R
Chemical type: Peptide, no natural source
Sequence: PKYVKQNTLKLAR
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 1.48

Epitope: PKY-11R
Chemical type: Peptide, no natural source
Sequence: PKYVKQNTLKLAR
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 1.83