Reference: Congling Qiu; Chanchan Xiao; Zhigang Wang; Guodong Zhu; Lipeng Mao; Xiongfei Chen; Lijuan Gao; Jieping Deng; Jun Su; Huanxing Su; Evandro Fei Fang; Zhang-Jin Zhang; Jikai Zhang; Caojun Xie; Jun Yuan; Oscar Junhong Luo; Li An Huang; Pengcheng Wang; Guobing Chen. "CD8<sup>+</sup> T-Cell Epitope Variations Suggest a Potential Antigen HLA-A2 Binding Deficiency for Spike Protein of SARS-CoV-2." Frontiers in immunology (2021).

Epitope: n-Sp1
Chemical type: Peptide from protein
Sequence: FVFLVLLPLV
Position: 2-11
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host:  23-53 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp1
Chemical type: Peptide from protein
Sequence: FVFLVLLPLV
Position: 2-11
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive-High
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: n-Sp1
Chemical type: Peptide from protein
Sequence: FVFLVLLPLV
Position: 2-11
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp1
Chemical type: Peptide from protein
Sequence: FVFLVLLPLV
Position: 2-11
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host:  23-77 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp1
Chemical type: Peptide from protein
Sequence: FVFLVLLPLV
Position: 2-11
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive-High

Epitope: n-Sp2
Chemical type: Peptide from protein
Sequence: FQFCNDPFL
Position: 133-141
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp2
Chemical type: Peptide from protein
Sequence: FQFCNDPFL
Position: 133-141
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host:  23-77 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp2
Chemical type: Peptide from protein
Sequence: FQFCNDPFL
Position: 133-141
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive

Epitope: n-Sp3
Chemical type: Peptide from protein
Sequence: YLQPRTFLL
Position: 269-277
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp3
Chemical type: Peptide from protein
Sequence: YLQPRTFLL
Position: 269-277
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Host:  23-77 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp3
Chemical type: Peptide from protein
Sequence: YLQPRTFLL
Position: 269-277
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive

Epitope: n-Sp4
Chemical type: Peptide from protein
Sequence: KLNDLCFTNV
Position: 386-395
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp4
Chemical type: Peptide from protein
Sequence: KLNDLCFTNV
Position: 386-395
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Host:  23-77 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp4
Chemical type: Peptide from protein
Sequence: KLNDLCFTNV
Position: 386-395
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive

Epitope: n-Sp5
Chemical type: Peptide from protein
Sequence: KIADYNYKL
Position: 417-425
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp5
Chemical type: Peptide from protein
Sequence: KIADYNYKL
Position: 417-425
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Host:  23-77 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp5
Chemical type: Peptide from protein
Sequence: KIADYNYKL
Position: 417-425
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Negative

Epitope: n-Sp6
Chemical type: Peptide from protein
Sequence: YQDVNCTEV
Position: 612-620
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp6
Chemical type: Peptide from protein
Sequence: YQDVNCTEV
Position: 612-620
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive-Low
Host:  23-77 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp6
Chemical type: Peptide from protein
Sequence: YQDVNCTEV
Position: 612-620
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive

Epitope: n-Sp7
Chemical type: Peptide from protein
Sequence: FTISVTTEI
Position: 718-726
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp7
Chemical type: Peptide from protein
Sequence: FTISVTTEI
Position: 718-726
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host:  23-77 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp7
Chemical type: Peptide from protein
Sequence: FTISVTTEI
Position: 718-726
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive

Epitope: n-Sp8
Chemical type: Peptide from protein
Sequence: KTSVDCTMYI
Position: 733-742
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp8
Chemical type: Peptide from protein
Sequence: KTSVDCTMYI
Position: 733-742
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Host:  23-77 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp8
Chemical type: Peptide from protein
Sequence: KTSVDCTMYI
Position: 733-742
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive

Epitope: n-Sp9
Chemical type: Peptide from protein
Sequence: MQMAYRFNGI
Position: 900-909
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp9
Chemical type: Peptide from protein
Sequence: MQMAYRFNGI
Position: 900-909
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Host:  23-77 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp9
Chemical type: Peptide from protein
Sequence: MQMAYRFNGI
Position: 900-909
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive

Epitope: n-Sp10
Chemical type: Peptide from protein
Sequence: RLQSLQTYV
Position: 1000-1008
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp10
Chemical type: Peptide from protein
Sequence: RLQSLQTYV
Position: 1000-1008
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Host:  23-77 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp10
Chemical type: Peptide from protein
Sequence: RLQSLQTYV
Position: 1000-1008
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive

Epitope: n-Sp11
Chemical type: Peptide from protein
Sequence: VVFLHVTYV
Position: 1060-1068
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp11
Chemical type: Peptide from protein
Sequence: VVFLHVTYV
Position: 1060-1068
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host:  23-77 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp11
Chemical type: Peptide from protein
Sequence: VVFLHVTYV
Position: 1060-1068
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive

Epitope: n-Sp12
Chemical type: Peptide from protein
Sequence: FVSNGTHWFV
Position: 1095-1104
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp12
Chemical type: Peptide from protein
Sequence: FVSNGTHWFV
Position: 1095-1104
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Host:  23-77 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp12
Chemical type: Peptide from protein
Sequence: FVSNGTHWFV
Position: 1095-1104
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Negative

Epitope: n-Sp13
Chemical type: Peptide from protein
Sequence: RLNEVAKNL
Position: 1185-1193
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp13
Chemical type: Peptide from protein
Sequence: RLNEVAKNL
Position: 1185-1193
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host:  23-77 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp13
Chemical type: Peptide from protein
Sequence: RLNEVAKNL
Position: 1185-1193
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive

Epitope: n-Sp14
Chemical type: Peptide from protein
Sequence: YIWLGFIAGL
Position: 1215-1224
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp14
Chemical type: Peptide from protein
Sequence: YIWLGFIAGL
Position: 1215-1224
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host:  23-77 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp14
Chemical type: Peptide from protein
Sequence: YIWLGFIAGL
Position: 1215-1224
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive

Epitope: n-Sp15
Chemical type: Peptide from protein
Sequence: FIAGLIAIV
Position: 1220-1228
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp15
Chemical type: Peptide from protein
Sequence: FIAGLIAIV
Position: 1220-1228
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Host:  23-77 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp15
Chemical type: Peptide from protein
Sequence: FIAGLIAIV
Position: 1220-1228
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive

Epitope: n-Sp1-m1
Chemical type: Peptide from protein
Sequence: FVFFVLLPLV
Position: 2-11
Source molecule (GenBank): QVE57165.1
Assay category: TCell
Result: Negative
Host:  23-77 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: n-Sp1-m1
Chemical type: Peptide from protein
Sequence: FVFFVLLPLV
Position: 2-11
Source molecule (GenBank): QVE57165.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: n-Sp1-m1
Chemical type: Peptide from protein
Sequence: FVFFVLLPLV
Position: 2-11
Source molecule (GenBank): QVE57165.1
Assay category: MhcBinding
Result: Positive-High

Epitope: n-Sp1-m2
Chemical type: Peptide from protein
Sequence: FVFLVLVPLV
Position: 2-11
Source molecule (GenBank): A0A6M3HLG5.1
Assay category: MhcBinding
Result: Negative

Epitope: n-Sp1-m3
Chemical type: Peptide from protein
Sequence: FVFFVLVPLV
Assay category: MhcBinding
Result: Negative

Epitope: n-Sp2-m1
Chemical type: Peptide from protein
Sequence: FQFCNHPFL
Assay category: MhcBinding
Result: Positive

Epitope: n-Sp2-m2
Chemical type: Peptide from protein
Sequence: FQFCNYPFL
Position: 133-141
Source molecule (GenBank): QWE88956.1
Assay category: MhcBinding
Result: Positive-Low

Epitope: n-Sp6-m1
Chemical type: Peptide from protein
Sequence: YQGVNCTEV
Position: 612-620
Source molecule (GenBank): QWZ01239.1
Assay category: MhcBinding
Result: Positive-Low