Reference: Daniel Parras; Patricia Solé; Thomas Delong; Pere Santamaría; Pau Serra. "Recognition of Multiple Hybrid Insulin Peptides by a Single Highly Diabetogenic T-Cell Receptor." Frontiers in immunology (2021).

Epitope: A5D
Chemical type: Peptide, no natural source
Sequence: KRLTLEVEDE
Assay category: TCell
Result: Positive-High
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: B2B
Chemical type: Peptide, no natural source
Sequence: VRLGLEVEDE
Assay category: TCell
Result: Positive-High
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: B2B
Chemical type: Peptide, no natural source
Sequence: VRLGLEVEDE
Assay category: TCell
Result: Positive
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: B2B
Chemical type: Peptide, no natural source
Sequence: VRLGLEVEDE
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B2B
Chemical type: Peptide, no natural source
Sequence: VRLGLEVEDE
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 7F
Chemical type: Peptide, no natural source
Sequence: WRYDLEVMAE
Assay category: TCell
Result: Positive
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: 11G
Chemical type: Peptide, no natural source
Sequence: SRLDLFVNLE
Assay category: TCell
Result: Positive
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: D8G
Chemical type: Peptide, no natural source
Sequence: RRMELYVYDE
Assay category: TCell
Result: Positive
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: 13
Chemical type: Peptide, no natural source
Sequence: VRLDLLVLEE
Assay category: TCell
Result: Positive
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: 8H
Chemical type: Peptide, no natural source
Sequence: TRLDLAVLDE
Assay category: TCell
Result: Positive-Low
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: 3B
Chemical type: Peptide, no natural source
Sequence: FRLSLDVLEE
Assay category: TCell
Result: Positive-Low
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: 10G
Chemical type: Peptide, no natural source
Sequence: WRELLSVNEE
Assay category: TCell
Result: Positive-Low
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: 1B
Chemical type: Peptide, no natural source
Sequence: GRLELIVLDEG
Assay category: TCell
Result: Positive-Low
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: HIP 15
Chemical type: Spliced peptide from proteins
Position: 75-79
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HIP 15
Chemical type: Spliced peptide from proteins
Position: 75-79
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Positive
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: HIP 15
Chemical type: Spliced peptide from proteins
Position: 75-79
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HIP 18
Chemical type: Spliced peptide from proteins
Position: 75-82
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Positive
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: HIP 18
Chemical type: Spliced peptide from proteins
Position: 75-82
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HIP 18
Chemical type: Spliced peptide from proteins
Position: 75-82
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HIP 30
Chemical type: Spliced peptide from proteins
Position: 75-80
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Positive
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: HIP 30
Chemical type: Spliced peptide from proteins
Position: 75-80
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HIP 30
Chemical type: Spliced peptide from proteins
Position: 75-80
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HIP 30 Q10E
Chemical type: Peptide, no natural source
Sequence: LQTLALEVEDPEV
Assay category: TCell
Result: Positive
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: HIP 30 Q10E
Chemical type: Peptide, no natural source
Sequence: LQTLALEVEDPEV
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HIP 30 Q10E
Chemical type: Peptide, no natural source
Sequence: LQTLALEVEDPEV
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HIP 32
Chemical type: Spliced peptide from proteins
Position: 75-81
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Positive
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: HIP 32
Chemical type: Spliced peptide from proteins
Position: 75-81
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HIP 32
Chemical type: Spliced peptide from proteins
Position: 75-81
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HIP 32Q9E
Chemical type: Peptide, no natural source
Sequence: LQTLALEAEDEEL
Assay category: TCell
Result: Positive
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: HIP 32Q9E
Chemical type: Peptide, no natural source
Sequence: LQTLALEAEDEEL
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HIP 32Q9E
Chemical type: Peptide, no natural source
Sequence: LQTLALEAEDEEL
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HIP 39
Chemical type: Spliced peptide from proteins
Position: 75-82
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Positive
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: HIP 39
Chemical type: Spliced peptide from proteins
Position: 75-82
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HIP 39
Chemical type: Spliced peptide from proteins
Position: 75-82
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HIP 40
Chemical type: Spliced peptide from proteins
Position: 75-82
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HIP 40
Chemical type: Spliced peptide from proteins
Position: 75-82
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HIP 43
Chemical type: Spliced peptide from proteins
Position: 65-69
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HIP 43
Chemical type: Spliced peptide from proteins
Position: 65-69
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HIP 55
Chemical type: Spliced peptide from proteins
Position: 65-70
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HIP 55
Chemical type: Spliced peptide from proteins
Position: 65-70
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HIP D1
Chemical type: Spliced peptide from proteins
Position: 75-83
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HIP D1
Chemical type: Spliced peptide from proteins
Position: 75-83
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HIP D2
Chemical type: Spliced peptide from proteins
Position: 63-70
Source molecule (GenBank): P01326.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HIP D2
Chemical type: Spliced peptide from proteins
Position: 63-70
Source molecule (GenBank): P01326.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo