Reference: Soo-Young Choi; Kee Woong Kwon; Hongmin Kim; Hong-Hee Choi; Sung Jae Shin. "Vaccine potential of ESAT-6 protein fused with consensus CD4<sup>+</sup> T-cell epitopes of PE/PPE proteins against highly pathogenic Mycobacterium tuberculosis strain HN878." Biochemical and biophysical research communications (2018).

Epitope: PE peptide 1
Chemical type: Peptide from protein
Sequence: MSFVTTQPEALAAAAGSLQGI
Position: 1-21
Source molecule (GenBank): YP_177834.1
Assay category: TCell
Result: Positive-High
Host: F 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PE peptide 2
Chemical type: Peptide from protein
Sequence: AATPTTGVVPAAADEVSALTA
Position: 32-52
Source molecule (GenBank): YP_177834.1
Assay category: TCell
Result: Negative
Host: F 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PPE peptide 3
Chemical type: Peptide from protein
Sequence: MDFGVLPPEINSGRMYAGPGS
Position: 1-21
Source molecule (GenBank): YP_177841.1
Assay category: TCell
Result: Positive-High
Host: F 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PPE peptide 4
Chemical type: Peptide from protein
Sequence: PMLAAAAAWDGLATELQSTAADYGSV
Position: 23-48
Source molecule (GenBank): YP_177841.1
Assay category: TCell
Result: Negative
Host: F 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PPE peptide 5
Chemical type: Peptide from protein
Sequence: LAATGYASVIAELTGAPWVGAASLSM
Position: 80-105
Source molecule (GenBank): YP_001283117.1
Assay category: TCell
Result: Positive-Low
Host: F 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PPE peptide 6
Chemical type: Peptide from protein
Sequence: WSGQSSGTMAAAAAPYVAWMS
Position: 56-76
Source molecule (GenBank): YP_177841.1
Assay category: TCell
Result: Negative
Host: F 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PPE peptide 7
Chemical type: Peptide from protein
Sequence: AGMQARAAAAAYELAFAMTVPP
Position: 86-107
Source molecule (GenBank): COV63394.1
Assay category: TCell
Result: Negative
Host: F 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PPE peptide 8
Chemical type: Peptide from protein
Sequence: AMTVPPPVVVANRALLVALVATN
Position: 102-124
Source molecule (GenBank): COV63394.1
Assay category: TCell
Result: Negative
Host: F 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PPE peptide 10
Chemical type: Peptide from protein
Sequence: LVATNFFGQNTPAIAATEAQY
Position: 120-140
Source molecule (GenBank): COV63394.1
Assay category: TCell
Result: Negative
Host: F 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PPE peptide 11
Chemical type: Peptide from protein
Sequence: IAATEAQYAEMWAQDAAAMYA
Position: 133-153
Source molecule (GenBank): COV63394.1
Assay category: TCell
Result: Negative
Host: F 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PPE peptide 12
Chemical type: Peptide from protein
Sequence: SASLARANKIGALSVPPSWVKTT
Position: 322-344
Source molecule (GenBank): YP_001283117.1
Assay category: TCell
Result: Negative
Host: F 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PE-PGRS peptide 13
Chemical type: Peptide from protein
Sequence: MSFVVTIPEALAAVATDLAGIG
Position: 21-42
Source molecule (GenBank): WP_021036386.1
Assay category: TCell
Result: Negative
Host: F 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PE-PGRS peptide 14
Chemical type: Peptide from protein
Sequence: STIGTANAAAAVPTTTVLAAA
Position: 43-63
Source molecule (GenBank): WP_021036386.1
Assay category: TCell
Result: Negative
Host: F 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PE-PGRS peptide 16
Chemical type: Peptide from protein
Sequence: AYQALSAQAALFMEQFVRALTAGAG
Assay category: TCell
Result: Negative
Host: F 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PE-PGRS peptide 17
Chemical type: Peptide from protein
Sequence: QFVRALTAGAGSYAAAEAASAAP
Position: 95-117
Source molecule (GenBank): WP_021036386.1
Assay category: TCell
Result: Negative
Host: F 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PE-PGRS peptide 18
Chemical type: Peptide from protein
Sequence: INAPVQSLTGRPLIGDGANG
Position: 104-123
Source molecule (GenBank): Q79FU0.1
Assay category: TCell
Result: Negative
Host: F 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PE-PGRS peptide 19
Chemical type: Peptide from protein
Sequence: SLTGRPLIGDGANGIDGTGQAGG
Position: 110-132
Source molecule (GenBank): Q79FU0.1
Assay category: TCell
Result: Negative
Host: F 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PE-PGRS peptide 20
Chemical type: Peptide from protein
Sequence: GGNGGWLWGNGGNGGSGAPGQ
Position: 131-151
Source molecule (GenBank): Q79FU0.1
Assay category: TCell
Result: Negative
Host: F 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo