Reference: Paula Y Arnold; Kate M Vignali; Timothy B Miller; Nicole L La Gruta; Linda S Cauley; Laura Haynes; P Scott Adams; Susan L Swain; David L Woodland; Dario A A Vignali. "Reliable generation and use of MHC class II:gamma2aFc multimers for the identification of antigen-specific CD4(+) T cells." Journal of immunological methods (2002).

Epitope: HEL 48-63
Chemical type: Peptide from protein
Sequence: DGSTDYGILQINSRWW
Position: 48-63
Source molecule (GenBank): 1LKS_A
Assay category: TCell
Result: Positive
Cell type: T cell
Tissue: spleen
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: HEL 48-63
Chemical type: Peptide from protein
Sequence: DGSTDYGILQINSRWW
Position: 48-63
Source molecule (GenBank): 1LKS_A
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HEL 48-63
Chemical type: Peptide from protein
Sequence: DGSTDYGILQINSRWW
Position: 48-63
Source molecule (GenBank): 1LKS_A
Assay category: MhcBinding
Result: Positive

Epitope: HEL 22-35
Chemical type: Peptide from protein
Sequence: GYSLGNWVCAAKFE
Position: 22-35
Source molecule (GenBank): 1LKS_A
Assay category: TCell
Result: Positive
Cell type: T cell
Tissue: lymphoid
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: HEL 22-35
Chemical type: Peptide from protein
Sequence: GYSLGNWVCAAKFE
Position: 22-35
Source molecule (GenBank): 1LKS_A
Assay category: MhcBinding
Result: Positive

Epitope: HEL 116-129
Chemical type: Peptide from protein
Sequence: KGTDVQAWIRGCRL
Position: 116-129
Source molecule (GenBank): 1LSB_A
Assay category: TCell
Result: Positive
Cell type: T cell
Tissue: lymphoid
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: HEL 116-129
Chemical type: Peptide from protein
Sequence: KGTDVQAWIRGCRL
Position: 116-129
Source molecule (GenBank): 1LSB_A
Assay category: MhcBinding
Result: Positive

Epitope: HEL 71-86
Chemical type: Peptide from protein
Sequence: GSRNLCNIPCSALLSS
Position: 71-86
Source molecule (GenBank): 1LKS_A
Assay category: MhcBinding
Result: Positive

Epitope: HEL 33-47
Chemical type: Peptide from protein
Sequence: KFESNFNTQATNRNT
Position: 33-47
Source molecule (GenBank): 1LKS_A
Assay category: MhcBinding
Result: Positive

Epitope: HEL 46-61
Chemical type: Peptide from protein
Sequence: NTDGSTDYGILQINSR
Position: 46-61
Source molecule (GenBank): 1LKS_A
Assay category: MhcBinding
Result: Positive

Epitope: HEL 4-16
Chemical type: Peptide from protein
Sequence: GRCELAAAMKRHG
Position: 4-16
Source molecule (GenBank): 1LKS_A
Assay category: TCell
Result: Positive
Cell type: T cell
Tissue: lymphoid
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: HEL 85-100
Chemical type: Peptide from protein
Sequence: SSDITASVNCAKKIVS
Position: 85-100
Source molecule (GenBank): 1LKS_A
Assay category: MhcBinding
Result: Positive

Epitope: HEL 105-119
Chemical type: Peptide from protein
Sequence: MNAWVAWRNRCKGTD
Position: 105-119
Source molecule (GenBank): 1LKS_A
Assay category: MhcBinding
Result: Positive

Epitope: HEL 81-97
Chemical type: Peptide from protein
Sequence: SALLSSDITASVNCAKK
Position: 81-97
Source molecule (GenBank): 1LKS_A
Assay category: MhcBinding
Result: Positive

Epitope: HEL 1-18
Chemical type: Peptide from protein
Sequence: KVFGRCELAAAMKRHGLD
Position: 1-18
Source molecule (GenBank): 1LKS_A
Assay category: TCell
Result: Positive
Cell type: T cell
Tissue: lymphoid
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: HEL 1-18
Chemical type: Peptide from protein
Sequence: KVFGRCELAAAMKRHGLD
Position: 1-18
Source molecule (GenBank): 1LKS_A
Assay category: MhcBinding
Result: Positive

Epitope: GAD 284-300
Chemical type: Peptide from protein
Sequence: SLKKGAAALGIGTDSVI
Position: 284-300
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Positive
Cell type: T cell
Tissue: lymphoid
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: GAD 284-300
Chemical type: Peptide from protein
Sequence: SLKKGAAALGIGTDSVI
Position: 284-300
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Positive

Epitope: GAD 206-220
Chemical type: Peptide from protein
Sequence: TYEIAPVFVLLEYVT
Position: 206-220
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Positive
Cell type: T cell
Tissue: lymphoid
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: GAD 206-220
Chemical type: Peptide from protein
Sequence: TYEIAPVFVLLEYVT
Position: 206-220
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Positive

Epitope: GAD 510-524
Chemical type: Peptide from protein
Sequence: PPSLRTLEDNEERMS
Position: 510-524
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Positive

Epitope: GAD 524-538
Chemical type: Peptide from protein
Sequence: SRLSKVAPVIKARMM
Position: 524-538
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Positive

Epitope: PCC 88-104
Chemical type: Peptide from protein
Sequence: KAERADLIAYLKQATAK
Position: 89-105
Source molecule (GenBank): P00021.2
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HN 419-433
Chemical type: Peptide from protein
Sequence: DRVYIYTRSSGWHSQ
Position: 419-433
Source molecule (GenBank): P04853.1
Assay category: TCell
Result: Positive
Quantitative measurement: 13.0
Host:  6-12 weeks
Cell type: T cell CD4+
Tissue: bronchial aveolar lavage (BAL)
Culture conditions: Direct Ex Vivo