Reference: Ting Liu; Jizhou Tan; Minhao Wu; Wenzhe Fan; Jialiang Wei; Bowen Zhu; Jian Guo; Shutong Wang; Penghui Zhou; Hui Zhang; Liangrong Shi; Jiaping Li. "High-affinity neoantigens correlate with better prognosis and trigger potent antihepatocellular carcinoma (HCC) activity by activating CD39<sup>+</sup>CD8<sup>+</sup> T cells." Gut (2021).

Epitope: Neoepitope VCX
Chemical type: Peptide, no natural source
Sequence: SQMEELPSV
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: Neoepitope VCX
Chemical type: Peptide, no natural source
Sequence: SQMEELPSV
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope CHST3
Chemical type: Peptide, no natural source
Sequence: WQLEFLQPL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: Neoepitope CHST3
Chemical type: Peptide, no natural source
Sequence: WQLEFLQPL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope OR10G8
Chemical type: Peptide, no natural source
Sequence: FILMGLPHV
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: Neoepitope OR10G8
Chemical type: Peptide, no natural source
Sequence: FILMGLPHV
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope OR10G8
Chemical type: Peptide, no natural source
Sequence: FILMGLPHV
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope DYNC1H1
Chemical type: Peptide, no natural source
Sequence: TLFSALWAL
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: Neoepitope DYNC1H1
Chemical type: Peptide, no natural source
Sequence: TLFSALWAL
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope CYB561D1
Chemical type: Peptide, no natural source
Sequence: WVMPVIPAL
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: Neoepitope CYB561D1
Chemical type: Peptide, no natural source
Sequence: WVMPVIPAL
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope TAS2R30
Chemical type: Peptide, no natural source
Sequence: RSLRLHRFR
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope SLC4A4
Chemical type: Peptide, no natural source
Sequence: STRPVLVFER
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope ABCA2
Chemical type: Peptide, no natural source
Sequence: FLLPSGMAL
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope GUCY1A3
Chemical type: Peptide, no natural source
Sequence: HVYYFFSKR
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope SLC26A11
Chemical type: Peptide, no natural source
Sequence: LMRDHMPPV
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope TAS1R1
Chemical type: Peptide, no natural source
Sequence: CLMHHLAQA
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope ADCY1
Chemical type: Peptide, no natural source
Sequence: VLYLHITWV
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope XKR4
Chemical type: Peptide, no natural source
Sequence: GLAPGLPSV
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: Neoepitope XKR4
Chemical type: Peptide, no natural source
Sequence: GLAPGLPSV
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope TAS2R46
Chemical type: Peptide, no natural source
Sequence: VLWHVTYWV
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: Neoepitope TAS2R46
Chemical type: Peptide, no natural source
Sequence: VLWHVTYWV
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope CSNK1A1
Chemical type: Peptide, no natural source
Sequence: YMYLRQLFHI
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: Neoepitope CSNK1A1
Chemical type: Peptide, no natural source
Sequence: YMYLRQLFHI
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope ANKRD36C
Chemical type: Peptide, no natural source
Sequence: TISKLYIPK
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: Neoepitope ANKRD36C
Chemical type: Peptide, no natural source
Sequence: TISKLYIPK
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope SYNPO
Chemical type: Peptide, no natural source
Sequence: QVWNPSFCFK
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope SYNPO
Chemical type: Peptide, no natural source
Sequence: QVWNPSFCFK
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: Neoepitope FOXB1
Chemical type: Peptide, no natural source
Sequence: MASGDHSAY
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope MIF4GD
Chemical type: Peptide, no natural source
Sequence: MPSHCNTQY
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope AP2A1
Chemical type: Peptide, no natural source
Sequence: FASDIPRIL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope SLC25A5
Chemical type: Peptide, no natural source
Sequence: TAVAGLASY
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope FRMD8
Chemical type: Peptide, no natural source
Sequence: LPWLSAHEL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope NCOA3
Chemical type: Peptide, no natural source
Sequence: HTFNCRMFMK
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope HEATR3
Chemical type: Peptide, no natural source
Sequence: SSMESRLLK
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope NPIPB15
Chemical type: Peptide, no natural source
Sequence: ATRINPWVEK
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope NBAS
Chemical type: Peptide, no natural source
Sequence: FYQSVISNPF
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Neoepitope RAG1
Chemical type: Peptide, no natural source
Sequence: MAASLPPTL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Wild-type VCX
Chemical type: Peptide from protein
Sequence: SEMEELPSV
Position: 238-246
Source molecule (GenBank): Q9H321.4
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: Wild-type CHST3
Chemical type: Peptide from protein
Sequence: RQLEFLQPL
Position: 278-286
Source molecule (GenBank): Q7LGC8.3
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: Wild-type OR10G8
Chemical type: Peptide from protein
Sequence: FILMGLPHA
Position: 10-18
Source molecule (GenBank): Q8NGN5.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: Wild-type DYNC1H1
Chemical type: Peptide from protein
Sequence: TLFSALRAL
Position: 2604-2612
Source molecule (GenBank): Q14204.5
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: Wild-type CYB561D1
Chemical type: Peptide from protein
Sequence: WVMPEIPAL
Position: 762-770
Source molecule (GenBank): EAW56387.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: Wild-type XKR4
Chemical type: Peptide from protein
Sequence: GLAPGLPSG
Position: 33-41
Source molecule (GenBank): Q5GH76.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: Wild-type TAS2R46
Chemical type: Peptide from protein
Sequence: VLWHVRYWV
Position: 293-301
Source molecule (GenBank): P59540.2
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: Wild-type CSNK1A1
Chemical type: Peptide from protein
Sequence: YMYLRQLFRI
Position: 274-283
Source molecule (GenBank): P48729.2
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: Wild-type ANKRD36C
Chemical type: Peptide from protein
Sequence: IISKLYIPK
Position: 368-376
Source molecule (GenBank): A6QL64.4
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: Wild-type SYNPO
Chemical type: Peptide from protein
Sequence: QVWKPSFCFK
Position: 920-929
Source molecule (GenBank): Q8N3V7.2
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: liver
Culture conditions: Direct Ex Vivo