Reference: Els Beirnaert; Aline Desmyter; Silvia Spinelli; Marc Lauwereys; Lucien Aarden; Torsten Dreier; Remy Loris; Karen Silence; Caroline Pollet; Christian Cambillau; Hans de Haard. "Bivalent Llama Single-Domain Antibody Fragments against Tumor Necrosis Factor Have Picomolar Potencies due to Intramolecular Interactions." Frontiers in immunology (2017).

Epitope: Epitope of VHH#2  on TNF
Chemical type: Discontinuous protein residues
Discontinuous residues: T153, T155, S157, Y163, Q164, T165, K166, V167, N168, I173, E211, I212, N213
Source molecule (GenBank): P01375.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.748

Epitope: Epitope of VHH#2  on TNF
Chemical type: Discontinuous protein residues
Discontinuous residues: T153, T155, S157, Y163, Q164, T165, K166, V167, N168, I173, E211, I212, N213
Source molecule (GenBank): P01375.1
Assay category: BCell
Result: Positive
Quantitative measurement: 1.9

Epitope: Epitope of VHH#2  on TNF
Chemical type: Discontinuous protein residues
Discontinuous residues: T153, T155, S157, Y163, Q164, T165, K166, V167, N168, I173, E211, I212, N213
Source molecule (GenBank): P01375.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.13

Epitope: Epitope of VHH#1 on TNF
Chemical type: Region on multi-chain protein
Discontinuous residues: B: T148, H149, V150, T153, T155, I159, Q164, T165, K166, V167, N168, I173, E211, I212, N213, R214, P215, D216; C: Y191, A221, E222, S223
Source molecule (GenBank): P01375.1
Assay category: BCell
Result: Positive

Epitope: Epitope of VHH#1 on TNF
Chemical type: Region on multi-chain protein
Discontinuous residues: B: T148, H149, V150, T153, T155, I159, Q164, T165, K166, V167, N168, I173, E211, I212, N213, R214, P215, D216; C: Y191, A221, E222, S223
Source molecule (GenBank): P01375.1
Assay category: BCell
Result: Positive
Quantitative measurement: 2.15

Epitope: Epitope of VHH#1 on TNF
Chemical type: Region on multi-chain protein
Discontinuous residues: B: T148, H149, V150, T153, T155, I159, Q164, T165, K166, V167, N168, I173, E211, I212, N213, R214, P215, D216; C: Y191, A221, E222, S223
Source molecule (GenBank): P01375.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.54

Epitope: Epitope of VHH#1 on TNF
Chemical type: Region on multi-chain protein
Discontinuous residues: B: T148, H149, V150, T153, T155, I159, Q164, T165, K166, V167, N168, I173, E211, I212, N213, R214, P215, D216; C: Y191, A221, E222, S223
Source molecule (GenBank): P01375.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.242

Epitope: Epitope of VHH#3  on TNF
Chemical type: Discontinuous protein residues
Discontinuous residues: P96, Q97, A98, E99, G100, Q101, K141, G142, Q143, P146,  E183, A187, R214, D216, Y217
Source molecule (GenBank): P01375.1
Assay category: BCell
Result: Positive
Quantitative measurement: 1.5

Epitope: Epitope of VHH#3  on TNF
Chemical type: Discontinuous protein residues
Discontinuous residues: P96, Q97, A98, E99, G100, Q101, K141, G142, Q143, P146,  E183, A187, R214, D216, Y217
Source molecule (GenBank): P01375.1
Assay category: BCell
Result: Positive
Quantitative measurement: 1.503

Epitope: Epitope of VHH#3  on TNF
Chemical type: Discontinuous protein residues
Discontinuous residues: P96, Q97, A98, E99, G100, Q101, K141, G142, Q143, P146,  E183, A187, R214, D216, Y217
Source molecule (GenBank): P01375.1
Assay category: BCell
Result: Positive

Epitope: Epitope of VHH#3  on TNF
Chemical type: Discontinuous protein residues
Discontinuous residues: P96, Q97, A98, E99, G100, Q101, K141, G142, Q143, P146,  E183, A187, R214, D216, Y217
Source molecule (GenBank): P01375.1
Assay category: BCell
Result: Positive
Quantitative measurement: 2.3

Epitope: Epitope of VHH#3  on TNF
Chemical type: Discontinuous protein residues
Discontinuous residues: P96, Q97, A98, E99, G100, Q101, K141, G142, Q143, P146,  E183, A187, R214, D216, Y217
Source molecule (GenBank): P01375.1
Assay category: BCell
Result: Positive