Reference: R A Ettinger; E A James; W W Kwok; A R Thompson; K P Pratt. "HLA-DR-restricted T-cell responses to factor VIII epitopes in a mild haemophilia A family with missense substitution A2201P." Haemophilia : the official journal of the World Federation of Hemophilia (2010).

Epitope: FVIII (484–508) A2-1
Chemical type: Peptide from protein
Sequence: RPLYSRRLPKGVKHLKDFPILPGEI
Position: 503-527
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (484–508) A2-1
Chemical type: Peptide from protein
Sequence: RPLYSRRLPKGVKHLKDFPILPGEI
Position: 503-527
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (484–508) A2-1
Chemical type: Peptide from protein
Sequence: RPLYSRRLPKGVKHLKDFPILPGEI
Position: 503-527
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (519-539) A2-2
Chemical type: Peptide from protein
Sequence: DGPTKSDPRCLTRYYSSFVN
Position: 538-557
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (519-539) A2-2
Chemical type: Peptide from protein
Sequence: DGPTKSDPRCLTRYYSSFVN
Position: 538-557
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (519-539) A2-2
Chemical type: Peptide from protein
Sequence: DGPTKSDPRCLTRYYSSFVN
Position: 538-557
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2170–2189)
Chemical type: Peptide from protein
Sequence: DLNSCSMPLGMESKAISDAQ
Position: 2189-2208
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2170–2189)
Chemical type: Peptide from protein
Sequence: DLNSCSMPLGMESKAISDAQ
Position: 2189-2208
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2170–2189)
Chemical type: Peptide from protein
Sequence: DLNSCSMPLGMESKAISDAQ
Position: 2189-2208
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2178–2197)
Chemical type: Peptide from protein
Sequence: LGMESKAISDAQITASSYFT
Position: 2197-2216
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2178–2197)
Chemical type: Peptide from protein
Sequence: LGMESKAISDAQITASSYFT
Position: 2197-2216
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2178–2197)
Chemical type: Peptide from protein
Sequence: LGMESKAISDAQITASSYFT
Position: 2197-2216
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2187-2205)
Chemical type: Peptide from protein
Sequence: DAQITASSYFTNMFATWSP
Position: 2206-2224
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2187-2205)
Chemical type: Peptide from protein
Sequence: DAQITASSYFTNMFATWSP
Position: 2206-2224
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2187-2205)
Chemical type: Peptide from protein
Sequence: DAQITASSYFTNMFATWSP
Position: 2206-2224
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2186-2205)
Chemical type: Peptide from protein
Sequence: SDAQITASSYFTNMFATWSP
Position: 2205-2224
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2186-2205)
Chemical type: Peptide from protein
Sequence: SDAQITASSYFTNMFATWSP
Position: 2205-2224
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2186-2205)
Chemical type: Peptide from protein
Sequence: SDAQITASSYFTNMFATWSP
Position: 2205-2224
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2194-2213)
Chemical type: Peptide from protein
Sequence: SYFTNMFATWSPSKARLHLQ
Position: 2213-2232
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: FVIII (2194-2213)
Chemical type: Peptide from protein
Sequence: SYFTNMFATWSPSKARLHLQ
Position: 2213-2232
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2194-2213)
Chemical type: Peptide from protein
Sequence: SYFTNMFATWSPSKARLHLQ
Position: 2213-2232
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2194-2213)
Chemical type: Peptide from protein
Sequence: SYFTNMFATWSPSKARLHLQ
Position: 2213-2232
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: FVIII (2202-2221)
Chemical type: Peptide from protein
Sequence: TWSPSKARLHLQGRSNAWRP
Position: 2221-2240
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2202-2221)
Chemical type: Peptide from protein
Sequence: TWSPSKARLHLQGRSNAWRP
Position: 2221-2240
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2202-2221)
Chemical type: Peptide from protein
Sequence: TWSPSKARLHLQGRSNAWRP
Position: 2221-2240
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2210-2229)
Chemical type: Peptide from protein
Sequence: LHLQGRSNAWRPQVNNPKEW
Position: 2229-2248
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2210-2229)
Chemical type: Peptide from protein
Sequence: LHLQGRSNAWRPQVNNPKEW
Position: 2229-2248
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2210-2229)
Chemical type: Peptide from protein
Sequence: LHLQGRSNAWRPQVNNPKEW
Position: 2229-2248
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2218-2237)
Chemical type: Peptide from protein
Sequence: AWRPQVNNPKEWLQVDFQKT
Position: 2237-2256
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2218-2237)
Chemical type: Peptide from protein
Sequence: AWRPQVNNPKEWLQVDFQKT
Position: 2237-2256
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2218-2237)
Chemical type: Peptide from protein
Sequence: AWRPQVNNPKEWLQVDFQKT
Position: 2237-2256
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: FVIII (2226-2245)
Chemical type: Peptide from protein
Sequence: PKEWLQVDFQKTMKVTGVTT
Position: 2245-2264
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2226-2245)
Chemical type: Peptide from protein
Sequence: PKEWLQVDFQKTMKVTGVTT
Position: 2245-2264
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2226-2245)
Chemical type: Peptide from protein
Sequence: PKEWLQVDFQKTMKVTGVTT
Position: 2245-2264
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2234-2253)
Chemical type: Peptide from protein
Sequence: FQKTMKVTGVTTQGVKSLLT
Position: 2253-2272
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2234-2253)
Chemical type: Peptide from protein
Sequence: FQKTMKVTGVTTQGVKSLLT
Position: 2253-2272
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2234-2253)
Chemical type: Peptide from protein
Sequence: FQKTMKVTGVTTQGVKSLLT
Position: 2253-2272
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2242-2261)
Chemical type: Peptide from protein
Sequence: GVTTQGVKSLLTSMYVKEFL
Position: 2261-2280
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2242-2261)
Chemical type: Peptide from protein
Sequence: GVTTQGVKSLLTSMYVKEFL
Position: 2261-2280
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2242-2261)
Chemical type: Peptide from protein
Sequence: GVTTQGVKSLLTSMYVKEFL
Position: 2261-2280
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2250-2269)
Chemical type: Peptide from protein
Sequence: SLLTSMYVKEFLISSSQDGH
Position: 2269-2288
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2250-2269)
Chemical type: Peptide from protein
Sequence: SLLTSMYVKEFLISSSQDGH
Position: 2269-2288
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2250-2269)
Chemical type: Peptide from protein
Sequence: SLLTSMYVKEFLISSSQDGH
Position: 2269-2288
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2258-2277)
Chemical type: Peptide from protein
Sequence: KEFLISSSQDGHQWTLFFQN
Position: 2277-2296
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2258-2277)
Chemical type: Peptide from protein
Sequence: KEFLISSSQDGHQWTLFFQN
Position: 2277-2296
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2258-2277)
Chemical type: Peptide from protein
Sequence: KEFLISSSQDGHQWTLFFQN
Position: 2277-2296
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2273-2292)
Chemical type: Peptide from protein
Sequence: LFFQNGKVKVFQGNQDSFTP
Position: 2292-2311
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2273-2292)
Chemical type: Peptide from protein
Sequence: LFFQNGKVKVFQGNQDSFTP
Position: 2292-2311
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2273-2292)
Chemical type: Peptide from protein
Sequence: LFFQNGKVKVFQGNQDSFTP
Position: 2292-2311
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2281-2300)
Chemical type: Peptide from protein
Sequence: KVFQGNQDSFTPVVNSLDPP
Position: 2300-2319
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2281-2300)
Chemical type: Peptide from protein
Sequence: KVFQGNQDSFTPVVNSLDPP
Position: 2300-2319
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2281-2300)
Chemical type: Peptide from protein
Sequence: KVFQGNQDSFTPVVNSLDPP
Position: 2300-2319
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2289-2308)
Chemical type: Peptide from protein
Sequence: SFTPVVNSLDPPLLTRYLRI
Position: 2308-2327
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2289-2308)
Chemical type: Peptide from protein
Sequence: SFTPVVNSLDPPLLTRYLRI
Position: 2308-2327
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2289-2308)
Chemical type: Peptide from protein
Sequence: SFTPVVNSLDPPLLTRYLRI
Position: 2308-2327
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2297-2316)
Chemical type: Peptide from protein
Sequence: LDPPLLTRYLRIHPQSWVHQ
Position: 2316-2335
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2297-2316)
Chemical type: Peptide from protein
Sequence: LDPPLLTRYLRIHPQSWVHQ
Position: 2316-2335
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2297-2316)
Chemical type: Peptide from protein
Sequence: LDPPLLTRYLRIHPQSWVHQ
Position: 2316-2335
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2305-2324)
Chemical type: Peptide from protein
Sequence: YLRIHPQSWVHQIALRMEVL
Position: 2324-2343
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2305-2324)
Chemical type: Peptide from protein
Sequence: YLRIHPQSWVHQIALRMEVL
Position: 2324-2343
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2305-2324)
Chemical type: Peptide from protein
Sequence: YLRIHPQSWVHQIALRMEVL
Position: 2324-2343
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2313–2332)
Chemical type: Peptide from protein
Sequence: WVHQIALRMEVLGCEAQDLY
Position: 2332-2351
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2313–2332)
Chemical type: Peptide from protein
Sequence: WVHQIALRMEVLGCEAQDLY
Position: 2332-2351
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2313–2332)
Chemical type: Peptide from protein
Sequence: WVHQIALRMEVLGCEAQDLY
Position: 2332-2351
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2317-2332)
Chemical type: Peptide from protein
Sequence: IALRMEVLGCEAQDLY
Position: 2336-2351
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2317-2332)
Chemical type: Peptide from protein
Sequence: IALRMEVLGCEAQDLY
Position: 2336-2351
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII (2317-2332)
Chemical type: Peptide from protein
Sequence: IALRMEVLGCEAQDLY
Position: 2336-2351
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII(2194–2213,2201P)
Chemical type: Peptide, no natural source
Sequence: SYFTNMFPTWSPSKARLHLQ
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII(2194–2213,2201P)
Chemical type: Peptide, no natural source
Sequence: SYFTNMFPTWSPSKARLHLQ
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII(2194–2213,2201P)
Chemical type: Peptide, no natural source
Sequence: SYFTNMFPTWSPSKARLHLQ
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated