Reference: Changrong Ge; Sylvia Weisse; Bingze Xu; Doreen Dobritzsch; Johan Viljanen; Jan Kihlberg; Nhu-Nguyen Do; Nadine Schneider; Harald Lanig; Rikard Holmdahl; Harald Burkhardt. "Key interactions in the trimolecular complex consisting of the rheumatoid arthritis-associated DRB1*04:01 molecule, the major glycosylated collagen II peptide and the T-cell receptor." Annals of the rheumatic diseases (2022).

Epitope: gal(264) Col2 (259-273)
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGEP
Position: 459-473
Source molecule (GenBank): P02458.3
Assay category: TCell
Result: Positive
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: gal(264) Col2 (259-273)
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGEP
Position: 459-473
Source molecule (GenBank): P02458.3
Assay category: TCell
Result: Positive-Intermediate
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: gal(264) Col2 (259-273)
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGEP
Position: 459-473
Source molecule (GenBank): P02458.3
Assay category: TCell
Result: Positive
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: gal(264) Col2 (259-273)
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGEP
Position: 459-473
Source molecule (GenBank): P02458.3
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: gal(264) Col2 (259-273)
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGEP
Position: 459-473
Source molecule (GenBank): P02458.3
Assay category: TCell
Result: Positive
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: gal(264) Col2 (259-273)
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGEP
Position: 459-473
Source molecule (GenBank): P02458.3
Assay category: TCell
Result: Positive
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: gal(264) Col2 (259-273)
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGEP
Position: 459-473
Source molecule (GenBank): P02458.3
Assay category: TCell
Result: Positive-Low
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: gal(264) Col2 (259-273)
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGEP
Position: 459-473
Source molecule (GenBank): P02458.3
Assay category: TCell
Result: Positive
MHC allele types: 63
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: gal(264) Col2 (259-273)
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGEP
Position: 459-473
Source molecule (GenBank): P02458.3
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: gal(264) Col2 (259-273)
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGEP
Position: 459-473
Source molecule (GenBank): P02458.3
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 2.05

Epitope: mut Col2 (259-273)
Chemical type: Peptide, no natural source
Sequence: GIAGFAGEQGPAGEP
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 1.9

Epitope: HSP70 (289-306)
Chemical type: Peptide from protein
Sequence: TRKPFQSVIADTGISV
Position: 289-304
Source molecule (GenBank): P0A5B9.2
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 2.24

Epitope: cit-CILP (982-996)
Chemical type: Peptide from protein
Sequence: GKLYGIRDVRSTRDR
Position: 982-996
Source molecule (GenBank): O75339.4
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 2.83

Epitope: nCol2 (259-273)
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGEP
Position: 459-473
Source molecule (GenBank): P02458.3
Assay category: TCell
Result: Positive-Intermediate
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: nCol2 (259-273)
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGEP
Position: 459-473
Source molecule (GenBank): P02458.3
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: nCol2 (259-273)
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGEP
Position: 459-473
Source molecule (GenBank): P02458.3
Assay category: TCell
Result: Positive-Low
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: nCol2 (259-273)
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGEP
Position: 459-473
Source molecule (GenBank): P02458.3
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: nCol2 (259-273)
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGEP
Position: 459-473
Source molecule (GenBank): P02458.3
Assay category: TCell
Result: Positive
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: nCol2 (259-273)
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGEP
Position: 459-473
Source molecule (GenBank): P02458.3
Assay category: TCell
Result: Negative
MHC allele types: 63
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo