Reference: David Oyen; Rainer Wechselberger; Vasundara Srinivasan; Jan Steyaert; John N Barlow. "Mechanistic analysis of allosteric and non-allosteric effects arising from nanobody binding to two epitopes of the dihydrofolate reductase of Escherichia coli." Biochimica et biophysica acta (2013).

Epitope: Epitope of nanobody Nb179 on DHFR
Chemical type: Discontinuous protein residues
Discontinuous residues: V10, D11, H114, D116, E118, D127, E129, P130, F140, C152, E154
Source molecule (GenBank): P0ABQ4.1
Assay category: BCell
Result: Positive

Epitope: Epitope of nanobody Nb179 on DHFR
Chemical type: Discontinuous protein residues
Discontinuous residues: V10, D11, H114, D116, E118, D127, E129, P130, F140, C152, E154
Source molecule (GenBank): P0ABQ4.1
Assay category: BCell
Result: Positive

Epitope: Epitope of nanobody Nb179 on DHFR
Chemical type: Discontinuous protein residues
Discontinuous residues: V10, D11, H114, D116, E118, D127, E129, P130, F140, C152, E154
Source molecule (GenBank): P0ABQ4.1
Assay category: BCell
Result: Positive
Quantitative measurement: 23.0

Epitope: Epitope of nanobody Nb179 on DHFR
Chemical type: Discontinuous protein residues
Discontinuous residues: V10, D11, H114, D116, E118, D127, E129, P130, F140, C152, E154
Source molecule (GenBank): P0ABQ4.1
Assay category: BCell
Result: Positive
Quantitative measurement: 710000.0

Epitope: Epitope of nanobody Nb179 on DHFR
Chemical type: Discontinuous protein residues
Discontinuous residues: V10, D11, H114, D116, E118, D127, E129, P130, F140, C152, E154
Source molecule (GenBank): P0ABQ4.1
Assay category: BCell
Result: Positive
Quantitative measurement: 2.8

Epitope: Epitope of nanobody Nb179 on DHFR
Chemical type: Discontinuous protein residues
Discontinuous residues: V10, D11, H114, D116, E118, D127, E129, P130, F140, C152, E154
Source molecule (GenBank): P0ABQ4.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.016

Epitope: Epitope of nanobody Ca1698 on DHFR
Chemical type: Discontinuous protein residues
Discontinuous residues: E17, N18, A19, M20, P21, W22, N23, L28, F31, E48, S49, I50, R52, L54, A145, Q146
Source molecule (GenBank): P0ABQ4.1
Assay category: BCell
Result: Positive

Epitope: Epitope of nanobody Ca1698 on DHFR
Chemical type: Discontinuous protein residues
Discontinuous residues: E17, N18, A19, M20, P21, W22, N23, L28, F31, E48, S49, I50, R52, L54, A145, Q146
Source molecule (GenBank): P0ABQ4.1
Assay category: BCell
Result: Positive
Quantitative measurement: 480000.0

Epitope: Epitope of nanobody Ca1698 on DHFR
Chemical type: Discontinuous protein residues
Discontinuous residues: E17, N18, A19, M20, P21, W22, N23, L28, F31, E48, S49, I50, R52, L54, A145, Q146
Source molecule (GenBank): P0ABQ4.1
Assay category: BCell
Result: Positive
Quantitative measurement: 2.5

Epitope: Epitope of nanobody Ca1698 on DHFR
Chemical type: Discontinuous protein residues
Discontinuous residues: E17, N18, A19, M20, P21, W22, N23, L28, F31, E48, S49, I50, R52, L54, A145, Q146
Source molecule (GenBank): P0ABQ4.1
Assay category: BCell
Result: Positive
Quantitative measurement: 1.0

Epitope: Epitope of nanobody Ca1698 on DHFR
Chemical type: Discontinuous protein residues
Discontinuous residues: E17, N18, A19, M20, P21, W22, N23, L28, F31, E48, S49, I50, R52, L54, A145, Q146
Source molecule (GenBank): P0ABQ4.1
Assay category: BCell
Result: Positive