Reference: Joshua S Woodworth; Daniel Shin; Mattijs Volman; Cláudio Nunes-Alves; Sarah M Fortune; Samuel M Behar. "Mycobacterium tuberculosis directs immunofocusing of CD8+ T cell responses despite vaccination." Journal of immunology (Baltimore, Md. : 1950) (2011).

Epitope: EspA Peptide 1
Chemical type: Peptide from protein
Sequence: GLYDLLGIGIPNQGGI
Position: 16-31
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 2
Chemical type: Peptide from protein
Sequence: LGIGIPNQGGILY
Position: 21-33
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 4
Chemical type: Peptide from protein
Sequence: PNQGGILYSSLEYF
Position: 26-39
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 5
Chemical type: Peptide from protein
Sequence: GGILYSSLEYFEKAL
Position: 29-43
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 6
Chemical type: Peptide from protein
Sequence: YSSLEYFEKALEELA
Position: 33-47
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 7
Chemical type: Peptide from protein
Sequence: AVDLTYIPVVGHAL
Position: 116-129
Source molecule (GenBank): NP_218133.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 8
Chemical type: Peptide from protein
Sequence: LTYIPVVGHALSAAF
Position: 119-133
Source molecule (GenBank): NP_218133.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 9
Chemical type: Peptide from protein
Sequence: PVVGHALSAAFQAPF
Position: 123-137
Source molecule (GenBank): NP_218133.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 10
Chemical type: Peptide from protein
Sequence: HALSAAFQAPFCAGA
Position: 127-141
Source molecule (GenBank): NP_218133.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 12
Chemical type: Peptide from protein
Sequence: APFCAGAMAVVGGAL
Position: 135-149
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 13
Chemical type: Peptide from protein
Sequence: AGAMAVVGGALAYLV
Position: 139-153
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 17
Chemical type: Peptide from protein
Sequence: VKTLINATQLLKLLA
Position: 154-168
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 19
Chemical type: Peptide from protein
Sequence: ALRPRADGPVGAAA
Position: 305-318
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 20
Chemical type: Peptide from protein
Sequence: PRADGPVGAAAEQV
Position: 308-321
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 21
Chemical type: Peptide from protein
Sequence: DGPVGAAAEQVGGQSQL
Position: 311-327
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 22
Chemical type: Peptide from protein
Sequence: AAEQVGGQSQLVSA
Position: 317-330
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 24
Chemical type: Peptide from protein
Sequence: VGGQSQLVSAQGSQGM
Position: 321-336
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 25
Chemical type: Peptide from protein
Sequence: SQLVSAQGSQGM
Position: 325-336
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 26
Chemical type: Peptide from protein
Sequence: LVSAQGSQGMGGPV
Position: 327-340
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 27
Chemical type: Peptide from protein
Sequence: AQGSQGMGGPVGM
Position: 330-342
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 29
Chemical type: Peptide from protein
Sequence: MGGPVGMGGMHPSSGA
Position: 336-351
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 30
Chemical type: Peptide from protein
Sequence: GMGGMHPSSGASK
Position: 341-353
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 31
Chemical type: Peptide from protein
Sequence: GGMHPSSGASKGTTTK
Position: 343-358
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 32
Chemical type: Peptide from protein
Sequence: SSGASKGTTTKKY
Position: 348-360
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 33
Chemical type: Peptide from protein
Sequence: GASKGTTTKKYSEGA
Position: 350-364
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 34
Chemical type: Peptide from protein
Sequence: GTTTKKYSEGAAA
Position: 354-366
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 35
Chemical type: Peptide from protein
Sequence: TTKKYSEGAAAGTEDA
Position: 356-371
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 36
Chemical type: Peptide from protein
Sequence: SEGAAAGTEDAERA
Position: 361-374
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 37
Chemical type: Peptide from protein
Sequence: AAAGTEDAERAPVEA
Position: 364-378
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 38
Chemical type: Peptide from protein
Sequence: TEDAERAPVEADA
Position: 368-380
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 39
Chemical type: Peptide from protein
Sequence: DAERAPVEADAGGGQK
Position: 370-385
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 40
Chemical type: Peptide from protein
Sequence: PVEADAGGGQKVLVR
Position: 375-389
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: EspA Peptide 41
Chemical type: Peptide from protein
Sequence: DAGGGQKVLVRNV
Position: 379-391
Source molecule (GenBank): ZP_07416836.1
Assay category: TCell
Result: Negative
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: Ag85A 142-161
Chemical type: Peptide from protein
Sequence: TFLTSELPGWLQANRHVKPT
Position: 142-161
Source molecule (GenBank): P0A4V2.1
Assay category: TCell
Result: Positive
Host: F 
Cell type: T cell CD4+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: TB10.4 74-88
Chemical type: Peptide from protein
Sequence: STHEANTMAMMARDT
Position: 74-88
Source molecule (GenBank): CAA17363.1
Assay category: TCell
Result: Positive
Host: F 
Cell type: T cell CD4+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: ESAT6 1-15
Chemical type: Peptide from protein
Sequence: MTEQQWNFAGIEAAA
Position: 1-15
Source molecule (GenBank): YP_178023.1
Assay category: TCell
Result: Positive
Host: F 
Cell type: T cell CD4+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: TB10.4 20-28
Chemical type: Peptide from protein
Sequence: GYAGTLQSL
Position: 20-28
Source molecule (GenBank): CAA17363.1
Assay category: TCell
Result: Positive
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: TB10.4 20-28
Chemical type: Peptide from protein
Sequence: GYAGTLQSL
Position: 20-28
Source molecule (GenBank): CAA17363.1
Assay category: TCell
Result: Positive
Host: F 
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TB10.4 20-28
Chemical type: Peptide from protein
Sequence: GYAGTLQSL
Position: 20-28
Source molecule (GenBank): CAA17363.1
Assay category: TCell
Result: Positive
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: TB10.4 20-28
Chemical type: Peptide from protein
Sequence: GYAGTLQSL
Position: 20-28
Source molecule (GenBank): CAA17363.1
Assay category: TCell
Result: Negative
Host: F

Epitope: TB10.4 20-28
Chemical type: Peptide from protein
Sequence: GYAGTLQSL
Position: 20-28
Source molecule (GenBank): CAA17363.1
Assay category: TCell
Result: Positive
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: Ag85A 113-121
Chemical type: Peptide from protein
Sequence: MPVGGQSSF
Position: 110-118
Source molecule (GenBank): P0C5B9.1
Assay category: TCell
Result: Positive
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: Ag85A 103-111
Chemical type: Peptide from protein
Sequence: WYDQSGLSV
Position: 103-111
Source molecule (GenBank): P0A4V2.1
Assay category: TCell
Result: Positive
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: Ag85A 187-195
Chemical type: Peptide from protein
Sequence: VYAGAMSG
Position: 187-194
Source molecule (GenBank): P0A4V2.1
Assay category: TCell
Result: Positive
Host: F 
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: F2<sub>26-34</sub>
Chemical type: Peptide from protein
Sequence: SPYAAGYDL
Position: 26-34
Source molecule (GenBank): YP_232923.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: lymph node
Culture conditions: Direct Ex Vivo