Reference: Lucie Loyal; Julian Braun; Larissa Henze; Beate Kruse; Manuela Dingeldey; Ulf Reimer; Florian Kern; Tatjana Schwarz; Maike Mangold; Clara Unger; Friederike Dörfler; Shirin Kadler; Jennifer Rosowski; Kübrah Gürcan; Zehra Uyar-Aydin; Marco Frentsch; Florian Kurth; Karsten Schnatbaum; Maren Eckey; Stefan Hippenstiel; Andreas Hocke; Marcel A Müller; Birgit Sawitzki; Stefan Miltenyi; Friedemann Paul; Marcus A Mall; Holger Wenschuh; Sebastian Voigt; Christian Drosten; Roland Lauster; Nils Lachman; Leif-Erik Sander; Victor M Corman; Jobst Röhmel; Lil Meyer-Arndt; Andreas Thiel; Claudia Giesecke-Thiel. "Cross-reactive CD4<sup>+</sup> T cells enhance SARS-CoV-2 immune responses upon infection and vaccination." Science (New York, N.Y.) (2021).

Epitope: CoV-2 S P204
Chemical type: Peptide from protein
Sequence: SKRSFIEDLLFNKVT
Position: 813-827
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S P204
Chemical type: Peptide from protein
Sequence: SKRSFIEDLLFNKVT
Position: 813-827
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S P205
Chemical type: Peptide from protein
Sequence: FIEDLLFNKVTLADA
Position: 817-831
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S P205
Chemical type: Peptide from protein
Sequence: FIEDLLFNKVTLADA
Position: 817-831
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S P186
Chemical type: Peptide from protein
Sequence: YICGDSTECSNLLLQ
Position: 741-755
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S P188
Chemical type: Peptide from protein
Sequence: CSNLLLQYGSFCTQL
Position: 749-763
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S P191
Chemical type: Peptide from protein
Sequence: TQLNRALTGIAVEQD
Position: 761-775
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S P192
Chemical type: Peptide from protein
Sequence: RALTGIAVEQDKNTQ
Position: 765-779
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S P198
Chemical type: Peptide from protein
Sequence: YKTPPIKDFGGFNFS
Position: 789-803
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S P199
Chemical type: Peptide from protein
Sequence: PIKDFGGFNFSQILP
Position: 793-807
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S P200
Chemical type: Peptide from protein
Sequence: FGGFNFSQILPDPSK
Position: 797-811
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S P201
Chemical type: Peptide from protein
Sequence: NFSQILPDPSKPSKR
Position: 801-815
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S P240
Chemical type: Peptide from protein
Sequence: QALNTLVKQLSSNFG
Position: 957-971
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S P243
Chemical type: Peptide from protein
Sequence: NFGAISSVLNDILSR
Position: 969-983
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S P244
Chemical type: Peptide from protein
Sequence: ISSVLNDILSRLDKV
Position: 973-987
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S P251
Chemical type: Peptide from protein
Sequence: LQSLQTYVTQQLIRA
Position: 1001-1015
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S P253
Chemical type: Peptide from protein
Sequence: TQQLIRAAEIRASAN
Position: 1009-1023
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S P254
Chemical type: Peptide from protein
Sequence: IRAAEIRASANLAAT
Position: 1013-1027
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S P256
Chemical type: Peptide from protein
Sequence: SANLAATKMSECVLG
Position: 1021-1035
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S P257
Chemical type: Peptide from protein
Sequence: AATKMSECVLGQSKR
Position: 1025-1039
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S P284
Chemical type: Peptide from protein
Sequence: VNNTVYDPLQPELDS
Position: 1133-1147
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S P284
Chemical type: Peptide from protein
Sequence: VNNTVYDPLQPELDS
Position: 1133-1147
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S 204_3
Chemical type: Peptide from protein
Sequence: SFIEDLLFNKVTLAD
Position: 816-830
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S 204_3
Chemical type: Peptide from protein
Sequence: SFIEDLLFNKVTLAD
Position: 816-830
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S 204_2
Chemical type: Peptide from protein
Sequence: SFIEDLLFNKVTLAD
Position: 816-830
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S 204_2
Chemical type: Peptide from protein
Sequence: SFIEDLLFNKVTLAD
Position: 816-830
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S 204_1
Chemical type: Peptide from protein
Sequence: SFIEDLLFNKVTLAD
Position: 816-830
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: CoV-2 S 204_1
Chemical type: Peptide from protein
Sequence: SFIEDLLFNKVTLAD
Position: 816-830
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated