Reference: Carla Oseroff; Bjoern Peters; Valerie Pasquetto; Magdalini Moutaftsi; John Sidney; Vijay Panchanathan; David C Tscharke; Bernard Maillere; Howard Grey; Alessandro Sette. "Dissociation between epitope hierarchy and immunoprevalence in CD8 responses to vaccinia virus western reserve." Journal of immunology (Baltimore, Md. : 1950) (2008).

Epitope: NC peptide 2
Chemical type: Peptide from protein
Sequence: KCENHRSVI
Position: 52-60
Source molecule (GenBank): P17358.1
Assay category: TCell
Result: Positive
Quantitative measurement: 73.3
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NC peptide 2
Chemical type: Peptide from protein
Sequence: KCENHRSVI
Position: 52-60
Source molecule (GenBank): P17358.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 6995.0

Epitope: NC peptide 2
Chemical type: Peptide from protein
Sequence: KCENHRSVI
Position: 52-60
Source molecule (GenBank): P17358.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 70000.0

Epitope: A3L (190–198)
Chemical type: Peptide from protein
Sequence: IYSPSNHHI
Position: 190-198
Source molecule (GenBank): AAO89401.1
Assay category: TCell
Result: Positive
Quantitative measurement: 632.5
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A3L (190–198)
Chemical type: Peptide from protein
Sequence: IYSPSNHHI
Position: 190-198
Source molecule (GenBank): AAO89401.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.9

Epitope: A3L (190–198)
Chemical type: Peptide from protein
Sequence: IYSPSNHHI
Position: 190-198
Source molecule (GenBank): AAO89401.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 17173.0

Epitope: A24R (522–530)
Chemical type: Peptide from protein
Sequence: SYFETGFPI
Position: 522-530
Source molecule (GenBank): Q76ZP7.1
Assay category: TCell
Result: Positive
Quantitative measurement: 294.6
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A24R (522–530)
Chemical type: Peptide from protein
Sequence: SYFETGFPI
Position: 522-530
Source molecule (GenBank): Q76ZP7.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.1

Epitope: A24R (522–530)
Chemical type: Peptide from protein
Sequence: SYFETGFPI
Position: 522-530
Source molecule (GenBank): Q76ZP7.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 8768.0

Epitope: A44L (114–122)
Chemical type: Peptide from protein
Sequence: IYTSSMEAI
Position: 114-122
Source molecule (GenBank): P26670.1
Assay category: TCell
Result: Positive
Quantitative measurement: 106.0
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A44L (114–122)
Chemical type: Peptide from protein
Sequence: IYTSSMEAI
Position: 114-122
Source molecule (GenBank): P26670.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 35870.0

Epitope: A44L (114–122)
Chemical type: Peptide from protein
Sequence: IYTSSMEAI
Position: 114-122
Source molecule (GenBank): P26670.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 2.3

Epitope: A47L (71–79)
Chemical type: Peptide from protein
Sequence: KIIQKSSSI
Position: 71-79
Source molecule (GenBank): P26673.2
Assay category: TCell
Result: Positive
Quantitative measurement: 56.7
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A47L (71–79)
Chemical type: Peptide from protein
Sequence: KIIQKSSSI
Position: 71-79
Source molecule (GenBank): P26673.2
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 70000.0

Epitope: A47L (71–79)
Chemical type: Peptide from protein
Sequence: KIIQKSSSI
Position: 71-79
Source molecule (GenBank): P26673.2
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 191.0

Epitope: A52R (75–83)
Chemical type: Peptide from protein
Sequence: KYGRLFNEI
Position: 75-83
Source molecule (GenBank): Q01220.1
Assay category: TCell
Result: Positive
Quantitative measurement: 1005.8
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A52R (75–83)
Chemical type: Peptide from protein
Sequence: KYGRLFNEI
Position: 75-83
Source molecule (GenBank): Q01220.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 18914.0

Epitope: A52R (75–83)
Chemical type: Peptide from protein
Sequence: KYGRLFNEI
Position: 75-83
Source molecule (GenBank): Q01220.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.2

Epitope: B2R (49–57)
Chemical type: Peptide from protein
Sequence: KYMWCYSQV
Position: 49-57
Source molecule (GenBank): Q01225.1
Assay category: TCell
Result: Positive
Quantitative measurement: 658.8
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B2R (49–57)
Chemical type: Peptide from protein
Sequence: KYMWCYSQV
Position: 49-57
Source molecule (GenBank): Q01225.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 70000.0

Epitope: B2R (49–57)
Chemical type: Peptide from protein
Sequence: KYMWCYSQV
Position: 49-57
Source molecule (GenBank): Q01225.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 22.0

Epitope: B10R (51–59)
Chemical type: Peptide from protein
Sequence: IYKDKQWSI
Position: 51-59
Source molecule (GenBank): Q76ZL3.1
Assay category: TCell
Result: Positive
Quantitative measurement: 170.8
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B10R (51–59)
Chemical type: Peptide from protein
Sequence: IYKDKQWSI
Position: 51-59
Source molecule (GenBank): Q76ZL3.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 70000.0

Epitope: B10R (51–59)
Chemical type: Peptide from protein
Sequence: IYKDKQWSI
Position: 51-59
Source molecule (GenBank): Q76ZL3.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 4350.0

Epitope: B16R (191–199)
Chemical type: Peptide from protein
Sequence: YYTCVLEYI
Position: 191-199
Source molecule (GenBank): P25212.1
Assay category: TCell
Result: Positive
Quantitative measurement: 215.2
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B16R (191–199)
Chemical type: Peptide from protein
Sequence: YYTCVLEYI
Position: 191-199
Source molecule (GenBank): P25212.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.3

Epitope: B16R (191–199)
Chemical type: Peptide from protein
Sequence: YYTCVLEYI
Position: 191-199
Source molecule (GenBank): P25212.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 5767.0

Epitope: B19R (304–312)
Chemical type: Peptide from protein
Sequence: SVLTSRGGI
Position: 304-312
Source molecule (GenBank): P25213.1
Assay category: TCell
Result: Positive
Quantitative measurement: 144.0
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B19R (304–312)
Chemical type: Peptide from protein
Sequence: SVLTSRGGI
Position: 304-312
Source molecule (GenBank): P25213.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 591.0

Epitope: B19R (304–312)
Chemical type: Peptide from protein
Sequence: SVLTSRGGI
Position: 304-312
Source molecule (GenBank): P25213.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 70000.0

Epitope: B22R/B28R/C22L(7-15)
Chemical type: Peptide from protein
Sequence: SYILFLSCI
Position: 7-15
Source molecule (GenBank): AAO89494.1
Assay category: TCell
Result: Positive
Quantitative measurement: 70.0
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B22R/B28R/C22L(7-15)
Chemical type: Peptide from protein
Sequence: SYILFLSCI
Position: 7-15
Source molecule (GenBank): AAO89494.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 68.0

Epitope: B22R/B28R/C22L(7-15)
Chemical type: Peptide from protein
Sequence: SYILFLSCI
Position: 7-15
Source molecule (GenBank): AAO89494.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 70000.0

Epitope: C6L (74–82)
Chemical type: Peptide from protein
Sequence: GFIRSLQTI
Position: 74-82
Source molecule (GenBank): P17362.1
Assay category: TCell
Result: Positive
Quantitative measurement: 989.4
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: C6L (74–82)
Chemical type: Peptide from protein
Sequence: GFIRSLQTI
Position: 74-82
Source molecule (GenBank): P17362.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.4

Epitope: C6L (74–82)
Chemical type: Peptide from protein
Sequence: GFIRSLQTI
Position: 74-82
Source molecule (GenBank): P17362.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 1153.0

Epitope: C10L (159–167)
Chemical type: Peptide from protein
Sequence: SYNRSMTTI
Position: 159-167
Source molecule (GenBank): P03296.1
Assay category: TCell
Result: Positive
Quantitative measurement: 115.8
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: C10L (159–167)
Chemical type: Peptide from protein
Sequence: SYNRSMTTI
Position: 159-167
Source molecule (GenBank): P03296.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.7

Epitope: C10L (159–167)
Chemical type: Peptide from protein
Sequence: SYNRSMTTI
Position: 159-167
Source molecule (GenBank): P03296.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 34745.0

Epitope: D1R (607–615)
Chemical type: Peptide from protein
Sequence: KYFYGEIAL
Position: 607-615
Source molecule (GenBank): P04298.1
Assay category: TCell
Result: Positive
Quantitative measurement: 70.4
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D1R (607–615)
Chemical type: Peptide from protein
Sequence: KYFYGEIAL
Position: 607-615
Source molecule (GenBank): P04298.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 36.0

Epitope: D1R (607–615)
Chemical type: Peptide from protein
Sequence: KYFYGEIAL
Position: 607-615
Source molecule (GenBank): P04298.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 70000.0

Epitope: D1R (797–805)
Chemical type: Peptide from protein
Sequence: KFINGASTM
Position: 797-805
Source molecule (GenBank): P04298.1
Assay category: TCell
Result: Positive
Quantitative measurement: 343.3
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D1R (797–805)
Chemical type: Peptide from protein
Sequence: KFINGASTM
Position: 797-805
Source molecule (GenBank): P04298.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 2.2

Epitope: D1R (797–805)
Chemical type: Peptide from protein
Sequence: KFINGASTM
Position: 797-805
Source molecule (GenBank): P04298.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 70000.0

Epitope: D1R (351–359)
Chemical type: Peptide from protein
Sequence: KYEGPFTTT
Position: 351-359
Source molecule (GenBank): P04298.1
Assay category: TCell
Result: Positive
Quantitative measurement: 47.9
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D1R (351–359)
Chemical type: Peptide from protein
Sequence: KYEGPFTTT
Position: 351-359
Source molecule (GenBank): P04298.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 2240.0

Epitope: D1R (351–359)
Chemical type: Peptide from protein
Sequence: KYEGPFTTT
Position: 351-359
Source molecule (GenBank): P04298.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 70000.0

Epitope: D4R (29–37)
Chemical type: Peptide from protein
Sequence: FYNEVASWL
Position: 29-37
Source molecule (GenBank): P04303.2
Assay category: TCell
Result: Positive
Quantitative measurement: 330.6
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D4R (29–37)
Chemical type: Peptide from protein
Sequence: FYNEVASWL
Position: 29-37
Source molecule (GenBank): P04303.2
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.1

Epitope: D4R (29–37)
Chemical type: Peptide from protein
Sequence: FYNEVASWL
Position: 29-37
Source molecule (GenBank): P04303.2
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 70000.0

Epitope: D5R (219–227)
Chemical type: Peptide from protein
Sequence: YYFSLQQRL
Position: 219-227
Source molecule (GenBank): P04305.1
Assay category: TCell
Result: Positive
Quantitative measurement: 89.2
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D5R (219–227)
Chemical type: Peptide from protein
Sequence: YYFSLQQRL
Position: 219-227
Source molecule (GenBank): P04305.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 1173.0

Epitope: D5R (219–227)
Chemical type: Peptide from protein
Sequence: YYFSLQQRL
Position: 219-227
Source molecule (GenBank): P04305.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 2.3

Epitope: D5R (251–259)
Chemical type: Peptide from protein
Sequence: SKIFINSII
Position: 251-259
Source molecule (GenBank): P04305.1
Assay category: TCell
Result: Positive
Quantitative measurement: 518.1
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D5R (251–259)
Chemical type: Peptide from protein
Sequence: SKIFINSII
Position: 251-259
Source molecule (GenBank): P04305.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 11247.0

Epitope: D5R (251–259)
Chemical type: Peptide from protein
Sequence: SKIFINSII
Position: 251-259
Source molecule (GenBank): P04305.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 87.0

Epitope: D5R (140–148)
Chemical type: Peptide from protein
Sequence: TYTTMDTLI
Position: 140-148
Source molecule (GenBank): P04305.1
Assay category: TCell
Result: Positive
Quantitative measurement: 296.8
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D5R (140–148)
Chemical type: Peptide from protein
Sequence: TYTTMDTLI
Position: 140-148
Source molecule (GenBank): P04305.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 1368.0

Epitope: D5R (140–148)
Chemical type: Peptide from protein
Sequence: TYTTMDTLI
Position: 140-148
Source molecule (GenBank): P04305.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 9.4

Epitope: D13L (61–69)
Chemical type: Peptide from protein
Sequence: QYITALNHL
Position: 61-69
Source molecule (GenBank): P68440.1
Assay category: TCell
Result: Positive
Quantitative measurement: 619.2
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D13L (61–69)
Chemical type: Peptide from protein
Sequence: QYITALNHL
Position: 61-69
Source molecule (GenBank): P68440.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 70000.0

Epitope: D13L (61–69)
Chemical type: Peptide from protein
Sequence: QYITALNHL
Position: 61-69
Source molecule (GenBank): P68440.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.8

Epitope: E2L (149–157)
Chemical type: Peptide from protein
Sequence: SYQFSENEI
Position: 149-157
Source molecule (GenBank): P21604.2
Assay category: TCell
Result: Positive
Quantitative measurement: 45.4
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E2L (149–157)
Chemical type: Peptide from protein
Sequence: SYQFSENEI
Position: 149-157
Source molecule (GenBank): P21604.2
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 70000.0

Epitope: E2L (149–157)
Chemical type: Peptide from protein
Sequence: SYQFSENEI
Position: 149-157
Source molecule (GenBank): P21604.2
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.8

Epitope: E6R (326–334)
Chemical type: Peptide from protein
Sequence: NFINGNTFI
Position: 326-334
Source molecule (GenBank): P21607.1
Assay category: TCell
Result: Positive
Quantitative measurement: 170.4
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E6R (326–334)
Chemical type: Peptide from protein
Sequence: NFINGNTFI
Position: 326-334
Source molecule (GenBank): P21607.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 24184.0

Epitope: E6R (326–334)
Chemical type: Peptide from protein
Sequence: NFINGNTFI
Position: 326-334
Source molecule (GenBank): P21607.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 2.1

Epitope: F1L (86–94)
Chemical type: Peptide from protein
Sequence: LPTKTRSYI
Position: 86-94
Source molecule (GenBank): P24356.2
Assay category: TCell
Result: Positive
Quantitative measurement: 186.0
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: F1L (86–94)
Chemical type: Peptide from protein
Sequence: LPTKTRSYI
Position: 86-94
Source molecule (GenBank): P24356.2
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 18539.0

Epitope: F1L (86–94)
Chemical type: Peptide from protein
Sequence: LPTKTRSYI
Position: 86-94
Source molecule (GenBank): P24356.2
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 890.0

Epitope: F4L (222–230)
Chemical type: Peptide from protein
Sequence: SIITDAVSI
Position: 222-230
Source molecule (GenBank): P11158.1
Assay category: TCell
Result: Positive
Quantitative measurement: 70.0
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: F4L (222–230)
Chemical type: Peptide from protein
Sequence: SIITDAVSI
Position: 222-230
Source molecule (GenBank): P11158.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 70000.0

Epitope: F4L (222–230)
Chemical type: Peptide from protein
Sequence: SIITDAVSI
Position: 222-230
Source molecule (GenBank): P11158.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 273.0

Epitope: F11L (158–166)
Chemical type: Peptide from protein
Sequence: LYIPGTSVI
Position: 158-166
Source molecule (GenBank): Q80HX7.1
Assay category: TCell
Result: Positive
Quantitative measurement: 298.3
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: F11L (158–166)
Chemical type: Peptide from protein
Sequence: LYIPGTSVI
Position: 158-166
Source molecule (GenBank): Q80HX7.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 70000.0

Epitope: F11L (158–166)
Chemical type: Peptide from protein
Sequence: LYIPGTSVI
Position: 158-166
Source molecule (GenBank): Q80HX7.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 2.3

Epitope: I4L (260–268)
Chemical type: Peptide from protein
Sequence: SYISGTNGI
Position: 260-268
Source molecule (GenBank): P12848.1
Assay category: TCell
Result: Positive
Quantitative measurement: 443.8
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: I4L (260–268)
Chemical type: Peptide from protein
Sequence: SYISGTNGI
Position: 260-268
Source molecule (GenBank): P12848.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 70000.0

Epitope: I4L (260–268)
Chemical type: Peptide from protein
Sequence: SYISGTNGI
Position: 260-268
Source molecule (GenBank): P12848.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.4

Epitope: I5L (53–61)
Chemical type: Peptide from protein
Sequence: KYIGLFIYI
Position: 53-61
Source molecule (GenBank): P12924.1
Assay category: TCell
Result: Positive
Quantitative measurement: 67.1
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: I5L (53–61)
Chemical type: Peptide from protein
Sequence: KYIGLFIYI
Position: 53-61
Source molecule (GenBank): P12924.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 70000.0

Epitope: I5L (53–61)
Chemical type: Peptide from protein
Sequence: KYIGLFIYI
Position: 53-61
Source molecule (GenBank): P12924.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 157.0

Epitope: I8R (511–519)
Chemical type: Peptide from protein
Sequence: SYMKSIQRI
Position: 511-519
Source molecule (GenBank): P12927.2
Assay category: TCell
Result: Positive
Quantitative measurement: 402.1
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: I8R (511–519)
Chemical type: Peptide from protein
Sequence: SYMKSIQRI
Position: 511-519
Source molecule (GenBank): P12927.2
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.1

Epitope: I8R (511–519)
Chemical type: Peptide from protein
Sequence: SYMKSIQRI
Position: 511-519
Source molecule (GenBank): P12927.2
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 70000.0

Epitope: I8R (90–98)
Chemical type: Peptide from protein
Sequence: QYIYSEHTI
Position: 90-98
Source molecule (GenBank): P12927.2
Assay category: TCell
Result: Positive
Quantitative measurement: 617.5
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: I8R (90–98)
Chemical type: Peptide from protein
Sequence: QYIYSEHTI
Position: 90-98
Source molecule (GenBank): P12927.2
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 2.5

Epitope: I8R (90–98)
Chemical type: Peptide from protein
Sequence: QYIYSEHTI
Position: 90-98
Source molecule (GenBank): P12927.2
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 21314.0

Epitope: J6R (386–394)
Chemical type: Peptide from protein
Sequence: RYNVIASSI
Position: 386-394
Source molecule (GenBank): P07392.1
Assay category: TCell
Result: Positive
Quantitative measurement: 50.4
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: J6R (386–394)
Chemical type: Peptide from protein
Sequence: RYNVIASSI
Position: 386-394
Source molecule (GenBank): P07392.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.1

Epitope: J6R (386–394)
Chemical type: Peptide from protein
Sequence: RYNVIASSI
Position: 386-394
Source molecule (GenBank): P07392.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 70000.0

Epitope: J6R (874–882)
Chemical type: Peptide from protein
Sequence: KYFFTVSNI
Position: 874-882
Source molecule (GenBank): P07392.1
Assay category: TCell
Result: Positive
Quantitative measurement: 140.0
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: J6R (874–882)
Chemical type: Peptide from protein
Sequence: KYFFTVSNI
Position: 874-882
Source molecule (GenBank): P07392.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 9.4

Epitope: J6R (874–882)
Chemical type: Peptide from protein
Sequence: KYFFTVSNI
Position: 874-882
Source molecule (GenBank): P07392.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 13876.0

Epitope: J6R (782–790)
Chemical type: Peptide from protein
Sequence: KYAANYTKI
Position: 782-790
Source molecule (GenBank): P07392.1
Assay category: TCell
Result: Positive
Quantitative measurement: 492.8
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: J6R (782–790)
Chemical type: Peptide from protein
Sequence: KYAANYTKI
Position: 782-790
Source molecule (GenBank): P07392.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.1

Epitope: J6R (782–790)
Chemical type: Peptide from protein
Sequence: KYAANYTKI
Position: 782-790
Source molecule (GenBank): P07392.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 70000.0

Epitope: N2L (145–153)
Chemical type: Peptide from protein
Sequence: KYKPVYSYV
Position: 145-153
Source molecule (GenBank): P14357.1
Assay category: TCell
Result: Positive
Quantitative measurement: 394.6
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N2L (145–153)
Chemical type: Peptide from protein
Sequence: KYKPVYSYV
Position: 145-153
Source molecule (GenBank): P14357.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.3

Epitope: N2L (145–153)
Chemical type: Peptide from protein
Sequence: KYKPVYSYV
Position: 145-153
Source molecule (GenBank): P14357.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 70000.0

Epitope: NC peptide 3
Chemical type: Peptide from protein
Sequence: IPAALIILL
Position: 148-156
Source molecule (GenBank): P24759.1
Assay category: TCell
Result: Positive
Quantitative measurement: 77.1
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NC peptide 3
Chemical type: Peptide from protein
Sequence: IPAALIILL
Position: 148-156
Source molecule (GenBank): P24759.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 55.0

Epitope: NC peptide 3
Chemical type: Peptide from protein
Sequence: IPAALIILL
Position: 148-156
Source molecule (GenBank): P24759.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 60.0

Epitope: NC peptide 3
Chemical type: Peptide from protein
Sequence: IPAALIILL
Position: 148-156
Source molecule (GenBank): P24759.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 18926.0

Epitope: C9L (379–387)
Chemical type: Peptide from protein
Sequence: MPLFPTFSM
Position: 379-387
Source molecule (GenBank): P17372.1
Assay category: TCell
Result: Positive
Quantitative measurement: 84.0
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: C9L (379–387)
Chemical type: Peptide from protein
Sequence: MPLFPTFSM
Position: 379-387
Source molecule (GenBank): P17372.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 18.0

Epitope: C9L (379–387)
Chemical type: Peptide from protein
Sequence: MPLFPTFSM
Position: 379-387
Source molecule (GenBank): P17372.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 35852.0

Epitope: C9L (379–387)
Chemical type: Peptide from protein
Sequence: MPLFPTFSM
Position: 379-387
Source molecule (GenBank): P17372.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 70000.0

Epitope: E9L (716–724)
Chemical type: Peptide from protein
Sequence: NPLSNPFYM
Position: 716-724
Source molecule (GenBank): P06856.4
Assay category: TCell
Result: Positive
Quantitative measurement: 222.3
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E9L (716–724)
Chemical type: Peptide from protein
Sequence: NPLSNPFYM
Position: 716-724
Source molecule (GenBank): P06856.4
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.6

Epitope: E9L (716–724)
Chemical type: Peptide from protein
Sequence: NPLSNPFYM
Position: 716-724
Source molecule (GenBank): P06856.4
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 11740.0

Epitope: E9L (716–724)
Chemical type: Peptide from protein
Sequence: NPLSNPFYM
Position: 716-724
Source molecule (GenBank): P06856.4
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 13144.0

Epitope: F2L (26–34)
Chemical type: Peptide from protein
Sequence: SPYAAGYDL
Position: 26-34
Source molecule (GenBank): AAO89320.1
Assay category: TCell
Result: Positive
Quantitative measurement: 900.6
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: F2L (26–34)
Chemical type: Peptide from protein
Sequence: SPYAAGYDL
Position: 26-34
Source molecule (GenBank): AAO89320.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 0.38

Epitope: F2L (26–34)
Chemical type: Peptide from protein
Sequence: SPYAAGYDL
Position: 26-34
Source molecule (GenBank): AAO89320.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 27686.0

Epitope: F2L (26–34)
Chemical type: Peptide from protein
Sequence: SPYAAGYDL
Position: 26-34
Source molecule (GenBank): AAO89320.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 32218.0

Epitope: I6L (159–167)
Chemical type: Peptide from protein
Sequence: IPMSIISFF
Position: 159-167
Source molecule (GenBank): P68462.1
Assay category: TCell
Result: Positive
Quantitative measurement: 90.4
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: I6L (159–167)
Chemical type: Peptide from protein
Sequence: IPMSIISFF
Position: 159-167
Source molecule (GenBank): P68462.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 2.3

Epitope: I6L (159–167)
Chemical type: Peptide from protein
Sequence: IPMSIISFF
Position: 159-167
Source molecule (GenBank): P68462.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 8059.0

Epitope: I6L (159–167)
Chemical type: Peptide from protein
Sequence: IPMSIISFF
Position: 159-167
Source molecule (GenBank): P68462.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 9587.0

Epitope: I8R (340–348)
Chemical type: Peptide from protein
Sequence: LPNPAFIHI
Position: 340-348
Source molecule (GenBank): P12927.2
Assay category: TCell
Result: Positive
Quantitative measurement: 140.6
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: I8R (340–348)
Chemical type: Peptide from protein
Sequence: LPNPAFIHI
Position: 340-348
Source molecule (GenBank): P12927.2
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.0

Epitope: I8R (340–348)
Chemical type: Peptide from protein
Sequence: LPNPAFIHI
Position: 340-348
Source molecule (GenBank): P12927.2
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 27134.0

Epitope: I8R (340–348)
Chemical type: Peptide from protein
Sequence: LPNPAFIHI
Position: 340-348
Source molecule (GenBank): P12927.2
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 16890.0

Epitope: L3L (221–229)
Chemical type: Peptide from protein
Sequence: LPQSCYLNF
Position: 221-229
Source molecule (GenBank): P07614.1
Assay category: TCell
Result: Positive
Quantitative measurement: 175.0
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: L3L (221–229)
Chemical type: Peptide from protein
Sequence: LPQSCYLNF
Position: 221-229
Source molecule (GenBank): P07614.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 7.4

Epitope: L3L (221–229)
Chemical type: Peptide from protein
Sequence: LPQSCYLNF
Position: 221-229
Source molecule (GenBank): P07614.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 6537.0

Epitope: L3L (221–229)
Chemical type: Peptide from protein
Sequence: LPQSCYLNF
Position: 221-229
Source molecule (GenBank): P07614.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 42762.0

Epitope: M1L (376–384)
Chemical type: Peptide from protein
Sequence: IPFIAYFVL
Position: 377-385
Source molecule (GenBank): P14356.1
Assay category: TCell
Result: Positive
Quantitative measurement: 135.0
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M1L (376–384)
Chemical type: Peptide from protein
Sequence: IPFIAYFVL
Position: 377-385
Source molecule (GenBank): P14356.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.5

Epitope: M1L (376–384)
Chemical type: Peptide from protein
Sequence: IPFIAYFVL
Position: 377-385
Source molecule (GenBank): P14356.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 48588.0

Epitope: M1L (376–384)
Chemical type: Peptide from protein
Sequence: IPFIAYFVL
Position: 377-385
Source molecule (GenBank): P14356.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 63859.0

Epitope: O1L (210–218)
Chemical type: Peptide from protein
Sequence: YPMDNVINF
Position: 210-218
Source molecule (GenBank): Q80HX1.1
Assay category: TCell
Result: Positive
Quantitative measurement: 100.4
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: O1L (210–218)
Chemical type: Peptide from protein
Sequence: YPMDNVINF
Position: 210-218
Source molecule (GenBank): Q80HX1.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 59978.0

Epitope: O1L (210–218)
Chemical type: Peptide from protein
Sequence: YPMDNVINF
Position: 210-218
Source molecule (GenBank): Q80HX1.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 4255.0

Epitope: O1L (210–218)
Chemical type: Peptide from protein
Sequence: YPMDNVINF
Position: 210-218
Source molecule (GenBank): Q80HX1.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 10.0

Epitope: A35R (31–39)
Chemical type: Peptide from protein
Sequence: IGPYIIGNI
Position: 31-39
Source molecule (GenBank): AAO89437.1
Assay category: TCell
Result: Positive
Quantitative measurement: 167.7
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A35R (31–39)
Chemical type: Peptide from protein
Sequence: IGPYIIGNI
Position: 31-39
Source molecule (GenBank): AAO89437.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 0.55

Epitope: A35R (31–39)
Chemical type: Peptide from protein
Sequence: IGPYIIGNI
Position: 31-39
Source molecule (GenBank): AAO89437.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 61401.0

Epitope: E3L (140–148)
Chemical type: Peptide from protein
Sequence: VGPSNSPTF
Position: 140-148
Source molecule (GenBank): P21605.1
Assay category: TCell
Result: Positive
Quantitative measurement: 771.0
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E3L (140–148)
Chemical type: Peptide from protein
Sequence: VGPSNSPTF
Position: 140-148
Source molecule (GenBank): P21605.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 0.93

Epitope: E3L (140–148)
Chemical type: Peptide from protein
Sequence: VGPSNSPTF
Position: 140-148
Source molecule (GenBank): P21605.1
Assay category: MhcBinding
Result: Negative