Reference: Zinal S Chheda; Gary Kohanbash; Kaori Okada; Naznin Jahan; John Sidney; Matteo Pecoraro; Xinbo Yang; Diego A Carrera; Kira M Downey; Shruti Shrivastav; Shuming Liu; Yi Lin; Chetana Lagisetti; Pavlina Chuntova; Payal B Watchmaker; Sabine Mueller; Ian F Pollack; Raja Rajalingam; Angel M Carcaboso; Matthias Mann; Alessandro Sette; K Christopher Garcia; Yafei Hou; Hideho Okada. "Novel and shared neoantigen derived from histone 3 variant H3.3K27M mutation for glioma T cell therapy." The Journal of experimental medicine (2018).

Epitope: H3.3 WT
Chemical type: Peptide from protein
Sequence: RKSAPSTGGV
Position: 27-36
Source molecule (GenBank): NP_002098.1
Assay category: TCell
Result: Negative
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 WT
Chemical type: Peptide from protein
Sequence: RKSAPSTGGV
Position: 27-36
Source molecule (GenBank): NP_002098.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: H3.3 WT
Chemical type: Peptide from protein
Sequence: RKSAPSTGGV
Position: 27-36
Source molecule (GenBank): NP_002098.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: H3.3 WT
Chemical type: Peptide from protein
Sequence: RKSAPSTGGV
Position: 27-36
Source molecule (GenBank): NP_002098.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 WT
Chemical type: Peptide from protein
Sequence: RKSAPSTGGV
Position: 27-36
Source molecule (GenBank): NP_002098.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: H3.3 WT
Chemical type: Peptide from protein
Sequence: RKSAPSTGGV
Position: 27-36
Source molecule (GenBank): NP_002098.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 38687.0

Epitope: H3.3 WT
Chemical type: Peptide from protein
Sequence: RKSAPSTGGV
Position: 27-36
Source molecule (GenBank): NP_002098.1
Assay category: MhcBinding
Result: Negative

Epitope: H3.3 WT
Chemical type: Peptide from protein
Sequence: RKSAPSTGGV
Position: 27-36
Source molecule (GenBank): NP_002098.1
Assay category: MhcBinding
Result: Negative

Epitope: H3.3 WT
Chemical type: Peptide from protein
Sequence: RKSAPSTGGV
Position: 27-36
Source molecule (GenBank): NP_002098.1
Assay category: MhcBinding
Result: Negative

Epitope: H3.3 WT
Chemical type: Peptide from protein
Sequence: RKSAPSTGGV
Position: 27-36
Source molecule (GenBank): NP_002098.1
Assay category: MhcBinding
Result: Negative

Epitope: H3.3 WT
Chemical type: Peptide from protein
Sequence: RKSAPSTGGV
Position: 27-36
Source molecule (GenBank): NP_002098.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 20601.0

Epitope: H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Positive
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Negative
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Positive
Quantitative measurement: 2900.0

Epitope: H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Positive
Cell type: T cell
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: MhcLigandElution
Result: Positive

Epitope: H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 151.0

Epitope: H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 29444.0

Epitope: H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 747.0

Epitope: H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: MhcBinding
Result: Negative

Epitope: H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 25615.0

Epitope: H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 18602.0

Epitope: H3.3 K27M-A2
Chemical type: Peptide, no natural source
Sequence: RASAPSTGGV
Assay category: TCell
Result: Negative
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 K27M-A2
Chemical type: Peptide, no natural source
Sequence: RASAPSTGGV
Assay category: MhcBinding
Result: Negative

Epitope: H3.3 K27M-A3
Chemical type: Peptide, no natural source
Sequence: RMAAPSTGGV
Assay category: TCell
Result: Positive
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 K27M-A3
Chemical type: Peptide, no natural source
Sequence: RMAAPSTGGV
Assay category: MhcBinding
Result: Positive-Intermediate

Epitope: H3.3 K27M-A4(V)
Chemical type: Peptide, no natural source
Sequence: RMSVPSTGGV
Assay category: TCell
Result: Negative
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 K27M-A4(V)
Chemical type: Peptide, no natural source
Sequence: RMSVPSTGGV
Assay category: MhcBinding
Result: Negative

Epitope: H3.3 K27M-A5
Chemical type: Peptide, no natural source
Sequence: RMSAASTGGV
Assay category: TCell
Result: Positive
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 K27M-A5
Chemical type: Peptide, no natural source
Sequence: RMSAASTGGV
Assay category: MhcBinding
Result: Positive-Intermediate

Epitope: H3.3 K27M-A6
Chemical type: Peptide, no natural source
Sequence: RMSAPATGGV
Assay category: TCell
Result: Negative
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 K27M-A6
Chemical type: Peptide, no natural source
Sequence: RMSAPATGGV
Assay category: MhcBinding
Result: Negative

Epitope: H3.3 K27M-A7
Chemical type: Peptide, no natural source
Sequence: RMSAPSAGGV
Assay category: TCell
Result: Negative
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 K27M-A7
Chemical type: Peptide, no natural source
Sequence: RMSAPSAGGV
Assay category: MhcBinding
Result: Negative

Epitope: H3.3 K27M-A8
Chemical type: Peptide, no natural source
Sequence: RMSAPSTAGV
Assay category: TCell
Result: Negative
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 K27M-A8
Chemical type: Peptide, no natural source
Sequence: RMSAPSTAGV
Assay category: MhcBinding
Result: Negative

Epitope: H3.3 K27M-A9
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGAV
Assay category: TCell
Result: Negative
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 K27M-A9
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGAV
Assay category: MhcBinding
Result: Negative

Epitope: Cit-H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Negative
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: Cit-H3.3 K27M
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: MhcBinding
Result: Positive-Intermediate