Reference: Eleanor Clancy-Thompson; Christine A Devlin; Paul M Tyler; Mariah M Servos; Lestat R Ali; Katherine S Ventre; M Aladdin Bhuiyan; Patrick T Bruck; Michael E Birnbaum; Stephanie K Dougan. "Altered Binding of Tumor Antigenic Peptides to MHC Class I Affects CD8<sup>+</sup> T Cell-Effector Responses." Cancer immunology research (2018).

Epitope: TRP1
Chemical type: Peptide from protein
Sequence: TAPDNLGYA
Position: 455-463
Source molecule (GenBank): P07147.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1
Chemical type: Peptide from protein
Sequence: TAPDNLGYA
Position: 455-463
Source molecule (GenBank): P07147.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1
Chemical type: Peptide from protein
Sequence: TAPDNLGYA
Position: 455-463
Source molecule (GenBank): P07147.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1
Chemical type: Peptide from protein
Sequence: TAPDNLGYA
Position: 455-463
Source molecule (GenBank): P07147.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1
Chemical type: Peptide from protein
Sequence: TAPDNLGYA
Position: 455-463
Source molecule (GenBank): P07147.1
Assay category: MhcBinding
Result: Positive-Low

Epitope: TRP1-M9
Chemical type: Peptide, no natural source
Sequence: TAPDNLGYM
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9
Chemical type: Peptide, no natural source
Sequence: TAPDNLGYM
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9
Chemical type: Peptide, no natural source
Sequence: TAPDNLGYM
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9
Chemical type: Peptide, no natural source
Sequence: TAPDNLGYM
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9
Chemical type: Peptide, no natural source
Sequence: TAPDNLGYM
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Cell Line / Clone

Epitope: TRP1-M9
Chemical type: Peptide, no natural source
Sequence: TAPDNLGYM
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9
Chemical type: Peptide, no natural source
Sequence: TAPDNLGYM
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Cell Line / Clone

Epitope: TRP1-M9
Chemical type: Peptide, no natural source
Sequence: TAPDNLGYM
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9
Chemical type: Peptide, no natural source
Sequence: TAPDNLGYM
Assay category: TCell
Result: Positive

Epitope: TRP1-M9
Chemical type: Peptide, no natural source
Sequence: TAPDNLGYM
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Cell Line / Clone

Epitope: TRP1-M9
Chemical type: Peptide, no natural source
Sequence: TAPDNLGYM
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9
Chemical type: Peptide, no natural source
Sequence: TAPDNLGYM
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 2.0

Epitope: TRP1-M9
Chemical type: Peptide, no natural source
Sequence: TAPDNLGYM
Assay category: MhcBinding
Result: Positive

Epitope: TRP1-M9
Chemical type: Peptide, no natural source
Sequence: TAPDNLGYM
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 45.5

Epitope: TRP1-M9-A1
Chemical type: Peptide, no natural source
Sequence: AAPDNLGYM
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-A1
Chemical type: Peptide, no natural source
Sequence: AAPDNLGYM
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-A1
Chemical type: Peptide, no natural source
Sequence: AAPDNLGYM
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-A1
Chemical type: Peptide, no natural source
Sequence: AAPDNLGYM
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-A1
Chemical type: Peptide, no natural source
Sequence: AAPDNLGYM
Assay category: MhcBinding
Result: Positive

Epitope: TRP1-M9-A3
Chemical type: Peptide, no natural source
Sequence: TAADNLGYM
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-A3
Chemical type: Peptide, no natural source
Sequence: TAADNLGYM
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-A3
Chemical type: Peptide, no natural source
Sequence: TAADNLGYM
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-A3
Chemical type: Peptide, no natural source
Sequence: TAADNLGYM
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-A3
Chemical type: Peptide, no natural source
Sequence: TAADNLGYM
Assay category: MhcBinding
Result: Positive-Low

Epitope: TRP1-M9-A4
Chemical type: Peptide, no natural source
Sequence: TAPANLGYM
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-A4
Chemical type: Peptide, no natural source
Sequence: TAPANLGYM
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-A4
Chemical type: Peptide, no natural source
Sequence: TAPANLGYM
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-A4
Chemical type: Peptide, no natural source
Sequence: TAPANLGYM
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-A4
Chemical type: Peptide, no natural source
Sequence: TAPANLGYM
Assay category: MhcBinding
Result: Positive

Epitope: TRP1-M9-K4
Chemical type: Peptide, no natural source
Sequence: TAPKNLGYM
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-K4
Chemical type: Peptide, no natural source
Sequence: TAPKNLGYM
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-K4
Chemical type: Peptide, no natural source
Sequence: TAPKNLGYM
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-K4
Chemical type: Peptide, no natural source
Sequence: TAPKNLGYM
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-K4
Chemical type: Peptide, no natural source
Sequence: TAPKNLGYM
Assay category: MhcBinding
Result: Positive

Epitope: TRP1-M9-E8
Chemical type: Peptide, no natural source
Sequence: TAPDNLGEM
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-E8
Chemical type: Peptide, no natural source
Sequence: TAPDNLGEM
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-E8
Chemical type: Peptide, no natural source
Sequence: TAPDNLGEM
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-E8
Chemical type: Peptide, no natural source
Sequence: TAPDNLGEM
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-E8
Chemical type: Peptide, no natural source
Sequence: TAPDNLGEM
Assay category: MhcBinding
Result: Positive

Epitope: TRP1-M9-F8
Chemical type: Peptide, no natural source
Sequence: TAPDNLGFM
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-F8
Chemical type: Peptide, no natural source
Sequence: TAPDNLGFM
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-F8
Chemical type: Peptide, no natural source
Sequence: TAPDNLGFM
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-F8
Chemical type: Peptide, no natural source
Sequence: TAPDNLGFM
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-F8
Chemical type: Peptide, no natural source
Sequence: TAPDNLGFM
Assay category: MhcBinding
Result: Positive

Epitope: TRP1-M9-S8
Chemical type: Peptide, no natural source
Sequence: TAPDNLGSM
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-S8
Chemical type: Peptide, no natural source
Sequence: TAPDNLGSM
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-S8
Chemical type: Peptide, no natural source
Sequence: TAPDNLGSM
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-S8
Chemical type: Peptide, no natural source
Sequence: TAPDNLGSM
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TRP1-M9-S8
Chemical type: Peptide, no natural source
Sequence: TAPDNLGSM
Assay category: MhcBinding
Result: Positive