Reference: Keshu Liu; Yangyang Sun; Liu Chen; Tao Yun; Zheng Ni; Weicheng Ye; Cun Zhang. "Novel epitopes identified from Tembusu virus NS3 protein induce cytotoxic T lymphocyte response." Veterinary microbiology (2022).

Epitope: TMUV E-1 301-309
Chemical type: Peptide from protein
Sequence: TYPMCSNTF
Position: 301-309
Source molecule (GenBank): AGL76709.1
Assay category: TCell
Result: Positive-Intermediate
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TMUV E-1 301-309
Chemical type: Peptide from protein
Sequence: TYPMCSNTF
Position: 301-309
Source molecule (GenBank): AGL76709.1
Assay category: TCell
Result: Negative
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TMUV E-1 301-309
Chemical type: Peptide from protein
Sequence: TYPMCSNTF
Position: 301-309
Source molecule (GenBank): AGL76709.1
Assay category: TCell
Result: Negative
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: TMUV E-2 333-341
Chemical type: Peptide from protein
Sequence: DGPCRVPIS
Position: 333-341
Source molecule (GenBank): AGL76709.1
Assay category: TCell
Result: Positive-Intermediate
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TMUV E-2 333-341
Chemical type: Peptide from protein
Sequence: DGPCRVPIS
Position: 333-341
Source molecule (GenBank): AGL76709.1
Assay category: TCell
Result: Negative
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TMUV E-2 333-341
Chemical type: Peptide from protein
Sequence: DGPCRVPIS
Position: 333-341
Source molecule (GenBank): AGL76709.1
Assay category: TCell
Result: Negative
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: TMUV E-3 338-346
Chemical type: Peptide from protein
Sequence: VPISMSADL
Position: 338-346
Source molecule (GenBank): AGL76709.1
Assay category: TCell
Result: Positive-Intermediate
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TMUV E-3 338-346
Chemical type: Peptide from protein
Sequence: VPISMSADL
Position: 338-346
Source molecule (GenBank): AGL76709.1
Assay category: TCell
Result: Negative
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TMUV E-3 338-346
Chemical type: Peptide from protein
Sequence: VPISMSADL
Position: 338-346
Source molecule (GenBank): AGL76709.1
Assay category: TCell
Result: Negative
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: TMUV E-4 377-385
Chemical type: Peptide from protein
Sequence: PPFGDSFIL
Position: 377-385
Source molecule (GenBank): AGL76709.1
Assay category: TCell
Result: Positive-Intermediate
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TMUV E-4 377-385
Chemical type: Peptide from protein
Sequence: PPFGDSFIL
Position: 377-385
Source molecule (GenBank): AGL76709.1
Assay category: TCell
Result: Negative
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TMUV E-4 377-385
Chemical type: Peptide from protein
Sequence: PPFGDSFIL
Position: 377-385
Source molecule (GenBank): AGL76709.1
Assay category: TCell
Result: Negative
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: TMUV E-5 413-421
Chemical type: Peptide from protein
Sequence: KGAQRMVAL
Position: 413-421
Source molecule (GenBank): AGL76709.1
Assay category: TCell
Result: Positive-Intermediate
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TMUV E-5 413-421
Chemical type: Peptide from protein
Sequence: KGAQRMVAL
Position: 413-421
Source molecule (GenBank): AGL76709.1
Assay category: TCell
Result: Negative
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TMUV E-5 413-421
Chemical type: Peptide from protein
Sequence: KGAQRMVAL
Position: 413-421
Source molecule (GenBank): AGL76709.1
Assay category: TCell
Result: Negative
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: TMUV NS1-6 224-232
Chemical type: Peptide from protein
Sequence: TWPETHTLW
Position: 1012-1020
Source molecule (GenBank): AIF73121.1
Assay category: TCell
Result: Positive-Low
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS1-6 224-232
Chemical type: Peptide from protein
Sequence: TWPETHTLW
Position: 1012-1020
Source molecule (GenBank): AIF73121.1
Assay category: TCell
Result: Negative
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS1-6 224-232
Chemical type: Peptide from protein
Sequence: TWPETHTLW
Position: 1012-1020
Source molecule (GenBank): AIF73121.1
Assay category: TCell
Result: Negative
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: TMUV NS1-7 294-302
Chemical type: Peptide from protein
Sequence: RGPSARTTT
Position: 1082-1090
Source molecule (GenBank): AIF73121.1
Assay category: TCell
Result: Positive-Low
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS1-7 294-302
Chemical type: Peptide from protein
Sequence: RGPSARTTT
Position: 1082-1090
Source molecule (GenBank): AIF73121.1
Assay category: TCell
Result: Negative
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS1-7 294-302
Chemical type: Peptide from protein
Sequence: RGPSARTTT
Position: 1082-1090
Source molecule (GenBank): AIF73121.1
Assay category: TCell
Result: Negative
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: TMUV NS1-9 336-344
Chemical type: Peptide from protein
Sequence: RPTAHGDDM
Position: 1124-1132
Source molecule (GenBank): YP_004734464.1
Assay category: TCell
Result: Negative
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS1-9 336-344
Chemical type: Peptide from protein
Sequence: RPTAHGDDM
Position: 1124-1132
Source molecule (GenBank): YP_004734464.1
Assay category: TCell
Result: Negative
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: TMUV NS3-10 33-41
Chemical type: Peptide from protein
Sequence: KYQAGVGVM
Position: 1531-1539
Source molecule (GenBank): YP_004734464.1
Assay category: TCell
Result: Negative
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS3-10 33-41
Chemical type: Peptide from protein
Sequence: KYQAGVGVM
Position: 1531-1539
Source molecule (GenBank): YP_004734464.1
Assay category: TCell
Result: Negative
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: TMUV NS3-10 33-41
Chemical type: Peptide from protein
Sequence: KYQAGVGVM
Position: 1531-1539
Source molecule (GenBank): YP_004734464.1
Assay category: TCell
Result: Positive-Low
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS3-11 175-183
Chemical type: Peptide from protein
Sequence: VPQVFDENM
Position: 1673-1681
Source molecule (GenBank): YP_004734464.1
Assay category: TCell
Result: Positive-Low
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS3-11 175-183
Chemical type: Peptide from protein
Sequence: VPQVFDENM
Position: 1673-1681
Source molecule (GenBank): YP_004734464.1
Assay category: TCell
Result: Negative
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS3-11 175-183
Chemical type: Peptide from protein
Sequence: VPQVFDENM
Position: 1673-1681
Source molecule (GenBank): YP_004734464.1
Assay category: TCell
Result: Negative
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: TMUV NS3-12 275-284
Chemical type: Peptide from protein
Sequence: RVPNYNLFIM
Position: 1773-1782
Source molecule (GenBank): YP_004734464.1
Assay category: TCell
Result: Positive-Low
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS3-12 275-284
Chemical type: Peptide from protein
Sequence: RVPNYNLFIM
Position: 1773-1782
Source molecule (GenBank): YP_004734464.1
Assay category: TCell
Result: Negative
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS3-12 275-284
Chemical type: Peptide from protein
Sequence: RVPNYNLFIM
Position: 1773-1782
Source molecule (GenBank): YP_004734464.1
Assay category: TCell
Result: Negative
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: TMUV NS3-13 299-307
Chemical type: Peptide from protein
Sequence: GYISTKVEL
Position: 1797-1805
Source molecule (GenBank): YP_004734464.1
Assay category: TCell
Result: Positive
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: TMUV NS3-13 299-307
Chemical type: Peptide from protein
Sequence: GYISTKVEL
Position: 1797-1805
Source molecule (GenBank): YP_004734464.1
Assay category: TCell
Result: Positive-High
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS3-14 566-574
Chemical type: Peptide from protein
Sequence: DGPSCNTVL
Position: 2064-2072
Source molecule (GenBank): AIF73121.1
Assay category: TCell
Result: Positive-High
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS3-14 566-574
Chemical type: Peptide from protein
Sequence: DGPSCNTVL
Position: 2064-2072
Source molecule (GenBank): AIF73121.1
Assay category: TCell
Result: Positive
Host: F 4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated