Reference: Daniele Mennonna; Cristina Maccalli; Michele C Romano; Claudio Garavaglia; Filippo Capocefalo; Roberta Bordoni; Marco Severgnini; Gianluca De Bellis; John Sidney; Alessandro Sette; Alessandro Gori; Renato Longhi; Marco Braga; Luca Ghirardelli; Ludovica Baldari; Elena Orsenigo; Luca Albarello; Elisabetta Zino; Katharina Fleischhauer; Gina Mazzola; Norma Ferrero; Antonio Amoroso; Giulia Casorati; Giorgio Parmiani; Paolo Dellabona. "T cell neoepitope discovery in colorectal cancer by high throughput profiling of somatic mutations in expressed genes." Gut. (2017).

Epitope: SMAD4m-1
Chemical type: Peptide, no natural source
Sequence: GDRFCLGQLSNAHRT
Assay category: TCell
Result: Positive
Host: F 63 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: SMAD4m-1
Chemical type: Peptide, no natural source
Sequence: GDRFCLGQLSNAHRT
Assay category: TCell
Result: Positive
Host: F 63 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: SMAD4m-1
Chemical type: Peptide, no natural source
Sequence: GDRFCLGQLSNAHRT
Assay category: TCell
Result: Positive
Host: F 63 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: SMAD4m-1
Chemical type: Peptide, no natural source
Sequence: GDRFCLGQLSNAHRT
Assay category: TCell
Result: Negative
Host: F 63 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: SMAD4m-1
Chemical type: Peptide, no natural source
Sequence: GDRFCLGQLSNAHRT
Assay category: TCell
Result: Positive
Host: F 63 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: SMAD4m-2
Chemical type: Peptide, no natural source
Sequence: CLGQLSNAHRTEAIE
Assay category: TCell
Result: Negative
MHC allele types: 143 257 259 307 629
Host: F 63 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: SMAD4m-3
Chemical type: Peptide, no natural source
Sequence: LSNAHRTEAIERARL
Assay category: TCell
Result: Negative
MHC allele types: 143 257 259 307 629
Host: F 63 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: SMAD4-1 WT
Chemical type: Peptide from protein
Sequence: GDRFCLGQLSNVHRT
Position: 359-373
Source molecule (GenBank): Q9BYG6.1
Assay category: TCell
Result: Negative
Host: F 63 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: APC WT
Chemical type: Peptide from protein
Sequence: NQTTQEADSANTLQIAEIKEKIGTRSAEDP
Position: 1290-1319
Source molecule (GenBank): NP_000029.2
Assay category: TCell
Result: Negative
MHC allele types: 211 216 195 23 629 798 64 424 358 365
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: SMAD4<sup>V370</sup> WT
Chemical type: Peptide from protein
Sequence: SGGDRFCLGQLSNVHRTEAIERARLH
Position: 357-382
Source molecule (GenBank): Q9BYG6.1
Assay category: TCell
Result: Negative
Host: F 63 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: SMAD4-mut 1.6
Chemical type: Peptide, no natural source
Sequence: CLGQLSNA
Assay category: TCell
Result: Positive
Host: F 63 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone