Reference: Jaewon Choi; Scott P Goulding; Brandon P Conn; Christopher D McGann; Jared L Dietze; Jessica Kohler; Divya Lenkala; Antoine Boudot; Daniel A Rothenberg; Paul J Turcott; John R Srouji; Kendra C Foley; Michael S Rooney; Marit M van Buuren; Richard B Gaynor; Jennifer G Abelin; Terri A Addona; Vikram R Juneja. "Systematic discovery and validation of T cell targets directed against oncogenic KRAS mutations." Cell reports methods (2021).

Epitope: WT-10mer-VVVGAGGVGK
Chemical type: Peptide from protein
Sequence: VVVGAGGVGK
Position: 7-16
Source molecule (GenBank): P01111.1
Assay category: MhcLigandElution
Result: Positive

Epitope: WT-10mer-VVVGAGGVGK
Chemical type: Peptide from protein
Sequence: VVVGAGGVGK
Position: 7-16
Source molecule (GenBank): P01111.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 82.0

Epitope: WT-10mer-VVVGAGGVGK
Chemical type: Peptide from protein
Sequence: VVVGAGGVGK
Position: 7-16
Source molecule (GenBank): P01111.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 12.4

Epitope: G12C-VVVGACGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGACGVGK
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: G12C-VVVGACGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGACGVGK
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: G12C-VVVGACGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGACGVGK
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: G12C-VVVGACGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGACGVGK
Assay category: MhcLigandElution
Result: Positive

Epitope: G12C-VVVGACGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGACGVGK
Assay category: MhcLigandElution
Result: Positive

Epitope: G12C-VVVGACGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGACGVGK
Assay category: MhcLigandElution
Result: Positive

Epitope: G12C-VVVGACGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGACGVGK
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.6

Epitope: G12C-VVVGACGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGACGVGK
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 69.3

Epitope: G12D-VVVGADGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: G12D-VVVGADGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: G12D-VVVGADGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: G12D-VVVGADGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: G12D-VVVGADGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive-High
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: G12D-VVVGADGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: MhcLigandElution
Result: Positive

Epitope: G12D-VVVGADGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: MhcLigandElution
Result: Positive

Epitope: G12D-VVVGADGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: MhcLigandElution
Result: Positive

Epitope: G12D-VVVGADGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 314.9

Epitope: G12D-VVVGADGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 33.1

Epitope: G12V-VVVGAVGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGAVGVGK
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: G12V-VVVGAVGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGAVGVGK
Assay category: TCell
Result: Positive
Cell type: T cell
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: G12V-VVVGAVGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGAVGVGK
Assay category: TCell
Result: Positive
Cell type: T cell
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: G12V-VVVGAVGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGAVGVGK
Assay category: TCell
Result: Positive-High
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: G12V-VVVGAVGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGAVGVGK
Assay category: TCell
Result: Positive-High
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: G12V-VVVGAVGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGAVGVGK
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: G12V-VVVGAVGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGAVGVGK
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: G12V-VVVGAVGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGAVGVGK
Assay category: TCell
Result: Positive
Cell type: T cell
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: G12V-VVVGAVGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGAVGVGK
Assay category: TCell
Result: Negative
Cell type: T cell
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: G12V-VVVGAVGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGAVGVGK
Assay category: MhcLigandElution
Result: Positive

Epitope: G12V-VVVGAVGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGAVGVGK
Assay category: MhcLigandElution
Result: Positive

Epitope: G12V-VVVGAVGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGAVGVGK
Assay category: MhcLigandElution
Result: Positive

Epitope: G12V-VVVGAVGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGAVGVGK
Assay category: MhcLigandElution
Result: Positive

Epitope: G12V-VVVGAVGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGAVGVGK
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 44.2

Epitope: G12V-VVVGAVGVGK
Chemical type: Peptide, no natural source
Sequence: VVVGAVGVGK
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 26.1

Epitope: G12V-VVGAVGVGK
Chemical type: Peptide, no natural source
Sequence: VVGAVGVGK
Assay category: MhcLigandElution
Result: Positive

Epitope: G12V-VVGAVGVGK
Chemical type: Peptide, no natural source
Sequence: VVGAVGVGK
Assay category: MhcLigandElution
Result: Positive

Epitope: G12V-VVGAVGVGK
Chemical type: Peptide, no natural source
Sequence: VVGAVGVGK
Assay category: MhcLigandElution
Result: Positive

Epitope: G12V-VVGAVGVGK
Chemical type: Peptide, no natural source
Sequence: VVGAVGVGK
Assay category: MhcLigandElution
Result: Positive

Epitope: G12V-VVGAVGVGK
Chemical type: Peptide, no natural source
Sequence: VVGAVGVGK
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.9

Epitope: G12V-VVGAVGVGK
Chemical type: Peptide, no natural source
Sequence: VVGAVGVGK
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 7.7

Epitope: G12R-VVGARGVGK
Chemical type: Peptide, no natural source
Sequence: VVGARGVGK
Assay category: MhcLigandElution
Result: Positive

Epitope: G12R-VVGARGVGK
Chemical type: Peptide, no natural source
Sequence: VVGARGVGK
Assay category: MhcLigandElution
Result: Negative

Epitope: G12R-VVGARGVGK
Chemical type: Peptide, no natural source
Sequence: VVGARGVGK
Assay category: MhcLigandElution
Result: Negative

Epitope: G12R-VVGARGVGK
Chemical type: Peptide, no natural source
Sequence: VVGARGVGK
Assay category: MhcLigandElution
Result: Negative

Epitope: G12D-GADGVGKSAL
Chemical type: Peptide, no natural source
Sequence: GADGVGKSAL
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: G12D-GADGVGKSAL
Chemical type: Peptide, no natural source
Sequence: GADGVGKSAL
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: G12D-GADGVGKSAL
Chemical type: Peptide, no natural source
Sequence: GADGVGKSAL
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: G12D-GADGVGKSAL
Chemical type: Peptide, no natural source
Sequence: GADGVGKSAL
Assay category: MhcLigandElution
Result: Positive

Epitope: G12D-GADGVGKSAL
Chemical type: Peptide, no natural source
Sequence: GADGVGKSAL
Assay category: MhcLigandElution
Result: Positive

Epitope: G12D-GADGVGKSAL
Chemical type: Peptide, no natural source
Sequence: GADGVGKSAL
Assay category: MhcLigandElution
Result: Positive

Epitope: G12D-GADGVGKSAL
Chemical type: Peptide, no natural source
Sequence: GADGVGKSAL
Assay category: MhcLigandElution
Result: Negative

Epitope: G12D-GADGVGKSAL
Chemical type: Peptide, no natural source
Sequence: GADGVGKSAL
Assay category: MhcLigandElution
Result: Negative

Epitope: G12R-GARGVGKSAL
Chemical type: Peptide, no natural source
Sequence: GARGVGKSAL
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: G12R-GARGVGKSAL
Chemical type: Peptide, no natural source
Sequence: GARGVGKSAL
Assay category: MhcLigandElution
Result: Positive

Epitope: G12R-GARGVGKSAL
Chemical type: Peptide, no natural source
Sequence: GARGVGKSAL
Assay category: MhcLigandElution
Result: Negative

Epitope: G12R-GARGVGKSAL
Chemical type: Peptide, no natural source
Sequence: GARGVGKSAL
Assay category: MhcLigandElution
Result: Negative

Epitope: G12R-GARGVGKSAL
Chemical type: Peptide, no natural source
Sequence: GARGVGKSAL
Assay category: MhcLigandElution
Result: Negative

Epitope: G12V-AVGVGKSAL
Chemical type: Peptide, no natural source
Sequence: AVGVGKSAL
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: G12V-AVGVGKSAL
Chemical type: Peptide, no natural source
Sequence: AVGVGKSAL
Assay category: MhcLigandElution
Result: Positive

Epitope: G12V-GAVGVGKSAL
Chemical type: Peptide, no natural source
Sequence: GAVGVGKSAL
Assay category: MhcLigandElution
Result: Negative

Epitope: G12V-GAVGVGKSAL
Chemical type: Peptide, no natural source
Sequence: GAVGVGKSAL
Assay category: MhcLigandElution
Result: Negative

Epitope: G12V-GAVGVGKSAL
Chemical type: Peptide, no natural source
Sequence: GAVGVGKSAL
Assay category: MhcLigandElution
Result: Positive

Epitope: G12V-GAVGVGKSAL
Chemical type: Peptide, no natural source
Sequence: GAVGVGKSAL
Assay category: MhcLigandElution
Result: Positive

Epitope: G12V-GAVGVGKSA
Chemical type: Peptide, no natural source
Sequence: GAVGVGKSA
Assay category: MhcLigandElution
Result: Positive

Epitope: G12V-GAVGVGKSA
Chemical type: Peptide, no natural source
Sequence: GAVGVGKSA
Assay category: MhcLigandElution
Result: Positive

Epitope: G12C-GACGVGKSA
Chemical type: Peptide, no natural source
Sequence: GACGVGKSA
Assay category: MhcLigandElution
Result: Positive

Epitope: G12D-GADGVGKSA
Chemical type: Peptide, no natural source
Sequence: GADGVGKSA
Assay category: MhcLigandElution
Result: Positive

Epitope: G12C-KLVVVGACGV
Chemical type: Peptide, no natural source
Sequence: KLVVVGACGV
Assay category: MhcLigandElution
Result: Negative

Epitope: G12V-KLVVVGAVGV
Chemical type: Peptide, no natural source
Sequence: KLVVVGAVGV
Assay category: MhcLigandElution
Result: Negative

Epitope: G12D-VVGADGVGK
Chemical type: Peptide, no natural source
Sequence: VVGADGVGK
Assay category: MhcLigandElution
Result: Negative

Epitope: G12D-VVGADGVGK
Chemical type: Peptide, no natural source
Sequence: VVGADGVGK
Assay category: MhcLigandElution
Result: Negative

Epitope: G12D-VVGADGVGK
Chemical type: Peptide, no natural source
Sequence: VVGADGVGK
Assay category: MhcLigandElution
Result: Negative

Epitope: G12D-VVGADGVGK
Chemical type: Peptide, no natural source
Sequence: VVGADGVGK
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 518.7

Epitope: G12D-VVGADGVGK
Chemical type: Peptide, no natural source
Sequence: VVGADGVGK
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 203.9

Epitope: G12C-VVGACGVGK
Chemical type: Peptide, no natural source
Sequence: VVGACGVGK
Assay category: MhcLigandElution
Result: Negative

Epitope: G12C-VVGACGVGK
Chemical type: Peptide, no natural source
Sequence: VVGACGVGK
Assay category: MhcLigandElution
Result: Negative

Epitope: G12C-VVGACGVGK
Chemical type: Peptide, no natural source
Sequence: VVGACGVGK
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 4.1

Epitope: G12C-VVGACGVGK
Chemical type: Peptide, no natural source
Sequence: VVGACGVGK
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 43.2

Epitope: G12C-GACGVGKSAL
Chemical type: Peptide, no natural source
Sequence: GACGVGKSAL
Assay category: MhcLigandElution
Result: Negative

Epitope: G12C-GACGVGKSAL
Chemical type: Peptide, no natural source
Sequence: GACGVGKSAL
Assay category: MhcLigandElution
Result: Negative

Epitope: G12C-GACGVGKSAL
Chemical type: Peptide, no natural source
Sequence: GACGVGKSAL
Assay category: MhcLigandElution
Result: Negative

Epitope: G12C-GACGVGKSAL
Chemical type: Peptide, no natural source
Sequence: GACGVGKSAL
Assay category: MhcLigandElution
Result: Negative

Epitope: G12C-ACGVGKSAL
Chemical type: Peptide, no natural source
Sequence: ACGVGKSAL
Assay category: MhcLigandElution
Result: Negative

Epitope: WT-9mer-VVGAGGVGK
Chemical type: Peptide from protein
Sequence: VVGAGGVGK
Position: 8-16
Source molecule (GenBank): P01111.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 125.1

Epitope: WT-9mer-VVGAGGVGK
Chemical type: Peptide from protein
Sequence: VVGAGGVGK
Position: 8-16
Source molecule (GenBank): P01111.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 90.6