Reference: Xin Yu Chen; De Zhou Li; Xiao Zhi Zhong; Bokun Chen; Zhi Liang Duan; Jin Sheng Wen. "Induction of multiple cytotoxic T lymphocyte responses in mice by a multiepitope DNA vaccine against dengue virus serotype 1." Microbiology and immunology (2016).

Epitope: NS4A (140-148)
Chemical type: Peptide from protein
Sequence: GLLFMILTV
Position: 2234-2242
Source molecule (GenBank): AHC98427.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4A (140-148)
Chemical type: Peptide from protein
Sequence: GLLFMILTV
Position: 2234-2242
Source molecule (GenBank): AHC98427.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4A (140-148)
Chemical type: Peptide from protein
Sequence: GLLFMILTV
Position: 2234-2242
Source molecule (GenBank): AHC98427.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4A (140-148)
Chemical type: Peptide from protein
Sequence: GLLFMILTV
Position: 2234-2242
Source molecule (GenBank): AHC98427.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4A (140-148)
Chemical type: Peptide from protein
Sequence: GLLFMILTV
Position: 2234-2242
Source molecule (GenBank): AHC98427.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 (183-181)
Chemical type: Peptide from protein
Sequence: LLMRTTWAL
Position: 2427-2435
Source molecule (GenBank): AHG06335.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 (183-181)
Chemical type: Peptide from protein
Sequence: LLMRTTWAL
Position: 2427-2435
Source molecule (GenBank): AHG06335.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 (183-181)
Chemical type: Peptide from protein
Sequence: LLMRTTWAL
Position: 2427-2435
Source molecule (GenBank): AHG06335.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 (183-181)
Chemical type: Peptide from protein
Sequence: LLMRTTWAL
Position: 2427-2435
Source molecule (GenBank): AHG06335.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 (183-181)
Chemical type: Peptide from protein
Sequence: LLMRTTWAL
Position: 2427-2435
Source molecule (GenBank): AHG06335.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4B (40-48)
Chemical type: Peptide from protein
Sequence: TLYAVATTI
Position: 2284-2292
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4B (40-48)
Chemical type: Peptide from protein
Sequence: TLYAVATTI
Position: 2284-2292
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4B (40-48)
Chemical type: Peptide from protein
Sequence: TLYAVATTI
Position: 2284-2292
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4B (40-48)
Chemical type: Peptide from protein
Sequence: TLYAVATTI
Position: 2284-2292
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4B (40-48)
Chemical type: Peptide from protein
Sequence: TLYAVATTI
Position: 2284-2292
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS2A (144-152)
Chemical type: Peptide from protein
Sequence: QLWATLLSL
Position: 1271-1279
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS2A (144-152)
Chemical type: Peptide from protein
Sequence: QLWATLLSL
Position: 1271-1279
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS2A (144-152)
Chemical type: Peptide from protein
Sequence: QLWATLLSL
Position: 1271-1279
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS2A (144-152)
Chemical type: Peptide from protein
Sequence: QLWATLLSL
Position: 1271-1279
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS2A (144-152)
Chemical type: Peptide from protein
Sequence: QLWATLLSL
Position: 1271-1279
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS2B (15-23)
Chemical type: Peptide from protein
Sequence: SILLSSLLK
Position: 1360-1368
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS2B (15-23)
Chemical type: Peptide from protein
Sequence: SILLSSLLK
Position: 1360-1368
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS2B (15-23)
Chemical type: Peptide from protein
Sequence: SILLSSLLK
Position: 1360-1368
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS2B (15-23)
Chemical type: Peptide from protein
Sequence: SILLSSLLK
Position: 1360-1368
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS2B (15-23)
Chemical type: Peptide from protein
Sequence: SILLSSLLK
Position: 1360-1368
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4B (159-167)
Chemical type: Peptide from protein
Sequence: VVYDAKFEK
Position: 2403-2411
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4B (159-167)
Chemical type: Peptide from protein
Sequence: VVYDAKFEK
Position: 2403-2411
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4B (159-167)
Chemical type: Peptide from protein
Sequence: VVYDAKFEK
Position: 2403-2411
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4B (159-167)
Chemical type: Peptide from protein
Sequence: VVYDAKFEK
Position: 2403-2411
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4B (159-167)
Chemical type: Peptide from protein
Sequence: VVYDAKFEK
Position: 2403-2411
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 (561-569)
Chemical type: Peptide from protein
Sequence: ALLATSIFK
Position: 3054-3062
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 (561-569)
Chemical type: Peptide from protein
Sequence: ALLATSIFK
Position: 3054-3062
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 (561-569)
Chemical type: Peptide from protein
Sequence: ALLATSIFK
Position: 3054-3062
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 (561-569)
Chemical type: Peptide from protein
Sequence: ALLATSIFK
Position: 3054-3062
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 (561-569)
Chemical type: Peptide from protein
Sequence: ALLATSIFK
Position: 3054-3062
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3 (99-107)
Chemical type: Peptide from protein
Sequence: AVEPGKNPK
Position: 1574-1582
Source molecule (GenBank): AHG06313.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3 (99-107)
Chemical type: Peptide from protein
Sequence: AVEPGKNPK
Position: 1574-1582
Source molecule (GenBank): AHG06313.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3 (99-107)
Chemical type: Peptide from protein
Sequence: AVEPGKNPK
Position: 1574-1582
Source molecule (GenBank): AHG06313.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3 (99-107)
Chemical type: Peptide from protein
Sequence: AVEPGKNPK
Position: 1574-1582
Source molecule (GenBank): AHG06313.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3 (99-107)
Chemical type: Peptide from protein
Sequence: AVEPGKNPK
Position: 1574-1582
Source molecule (GenBank): AHG06313.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 (505-513)
Chemical type: Peptide from protein
Sequence: GVEGEGLHK
Position: 2998-3006
Source molecule (GenBank): ACJ04228.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 (505-513)
Chemical type: Peptide from protein
Sequence: GVEGEGLHK
Position: 2998-3006
Source molecule (GenBank): ACJ04228.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 (505-513)
Chemical type: Peptide from protein
Sequence: GVEGEGLHK
Position: 2998-3006
Source molecule (GenBank): ACJ04228.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 (505-513)
Chemical type: Peptide from protein
Sequence: GVEGEGLHK
Position: 2998-3006
Source molecule (GenBank): ACJ04228.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 (505-513)
Chemical type: Peptide from protein
Sequence: GVEGEGLHK
Position: 2998-3006
Source molecule (GenBank): ACJ04228.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E (39-47)
Chemical type: Peptide from protein
Sequence: PTLDIELLK
Position: 319-327
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E (39-47)
Chemical type: Peptide from protein
Sequence: PTLDIELLK
Position: 319-327
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E (39-47)
Chemical type: Peptide from protein
Sequence: PTLDIELLK
Position: 319-327
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E (39-47)
Chemical type: Peptide from protein
Sequence: PTLDIELLK
Position: 319-327
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E (39-47)
Chemical type: Peptide from protein
Sequence: PTLDIELLK
Position: 319-327
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 (880-888)
Chemical type: Peptide from protein
Sequence: DYMTSMKRF
Position: 3373-3381
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 (880-888)
Chemical type: Peptide from protein
Sequence: DYMTSMKRF
Position: 3373-3381
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 (880-888)
Chemical type: Peptide from protein
Sequence: DYMTSMKRF
Position: 3373-3381
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 (880-888)
Chemical type: Peptide from protein
Sequence: DYMTSMKRF
Position: 3373-3381
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 (880-888)
Chemical type: Peptide from protein
Sequence: DYMTSMKRF
Position: 3373-3381
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3 (548-556)
Chemical type: Peptide from protein
Sequence: SYKVASEGF
Position: 2023-2031
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3 (548-556)
Chemical type: Peptide from protein
Sequence: SYKVASEGF
Position: 2023-2031
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3 (548-556)
Chemical type: Peptide from protein
Sequence: SYKVASEGF
Position: 2023-2031
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3 (548-556)
Chemical type: Peptide from protein
Sequence: SYKVASEGF
Position: 2023-2031
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3 (548-556)
Chemical type: Peptide from protein
Sequence: SYKVASEGF
Position: 2023-2031
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4A (40-48)
Chemical type: Peptide from protein
Sequence: AYRHAMEEL
Position: 2134-2142
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4A (40-48)
Chemical type: Peptide from protein
Sequence: AYRHAMEEL
Position: 2134-2142
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4A (40-48)
Chemical type: Peptide from protein
Sequence: AYRHAMEEL
Position: 2134-2142
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4A (40-48)
Chemical type: Peptide from protein
Sequence: AYRHAMEEL
Position: 2134-2142
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4A (40-48)
Chemical type: Peptide from protein
Sequence: AYRHAMEEL
Position: 2134-2142
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3 (22-30)
Chemical type: Peptide from protein
Sequence: IYRILQRGL
Position: 1497-1505
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3 (22-30)
Chemical type: Peptide from protein
Sequence: IYRILQRGL
Position: 1497-1505
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3 (22-30)
Chemical type: Peptide from protein
Sequence: IYRILQRGL
Position: 1497-1505
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3 (22-30)
Chemical type: Peptide from protein
Sequence: IYRILQRGL
Position: 1497-1505
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3 (22-30)
Chemical type: Peptide from protein
Sequence: IYRILQRGL
Position: 1497-1505
Source molecule (GenBank): AEZ00065.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo