Reference: Jeffrey R Abbott; Guy H Palmer; Chris J Howard; Jayne C Hope; Wendy C Brown. "Anaplasma marginale major surface protein 2 CD4+-T-cell epitopes are evenly distributed in conserved and hypervariable regions (HVR), whereas linear B-cell epitopes are predominantly located in the HVR." Infection and immunity (2004).

Epitope: P1
Chemical type: Peptide from protein
Sequence: MSAVSNRKLPLGGVLMALAAAVAPIHSLLA
Position: 1-30
Source molecule (GenBank): AAA58953.1
Assay category: BCell
Result: Negative

Epitope: P1
Chemical type: Peptide from protein
Sequence: MSAVSNRKLPLGGVLMALAAAVAPIHSLLA
Position: 1-30
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P1
Chemical type: Peptide from protein
Sequence: MSAVSNRKLPLGGVLMALAAAVAPIHSLLA
Position: 1-30
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P2
Chemical type: Peptide from protein
Sequence: AVAPIHSLLAAPAAGAGAGGEGLFSGAGAG
Position: 21-50
Source molecule (GenBank): AAA58953.1
Assay category: BCell
Result: Negative

Epitope: P2
Chemical type: Peptide from protein
Sequence: AVAPIHSLLAAPAAGAGAGGEGLFSGAGAG
Position: 21-50
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P2
Chemical type: Peptide from protein
Sequence: AVAPIHSLLAAPAAGAGAGGEGLFSGAGAG
Position: 21-50
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P3
Chemical type: Peptide from protein
Sequence: EGLFSGAGAGSFYIGLDYSPAFGSIKDFKV
Position: 41-70
Source molecule (GenBank): AAA58953.1
Assay category: BCell
Result: Negative

Epitope: P3
Chemical type: Peptide from protein
Sequence: EGLFSGAGAGSFYIGLDYSPAFGSIKDFKV
Position: 41-70
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P3
Chemical type: Peptide from protein
Sequence: EGLFSGAGAGSFYIGLDYSPAFGSIKDFKV
Position: 41-70
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P4
Chemical type: Peptide from protein
Sequence: AFGSIKDFKVQEAGGTTRGVFPYKRDAAGR
Position: 61-90
Source molecule (GenBank): AAA58953.1
Assay category: BCell
Result: Positive

Epitope: P4
Chemical type: Peptide from protein
Sequence: AFGSIKDFKVQEAGGTTRGVFPYKRDAAGR
Position: 61-90
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P4
Chemical type: Peptide from protein
Sequence: AFGSIKDFKVQEAGGTTRGVFPYKRDAAGR
Position: 61-90
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P6
Chemical type: Peptide from protein
Sequence: SAPEPKISFKDSMLTALEGSIGYSIGGARV
Position: 101-130
Source molecule (GenBank): AAA58953.1
Assay category: BCell
Result: Positive

Epitope: P6
Chemical type: Peptide from protein
Sequence: SAPEPKISFKDSMLTALEGSIGYSIGGARV
Position: 101-130
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P6
Chemical type: Peptide from protein
Sequence: SAPEPKISFKDSMLTALEGSIGYSIGGARV
Position: 101-130
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P7
Chemical type: Peptide from protein
Sequence: IGYSIGGARVEVEVGYERFVIKGGKKSNED
Position: 121-150
Source molecule (GenBank): AAA58953.1
Assay category: BCell
Result: Negative

Epitope: P7
Chemical type: Peptide from protein
Sequence: IGYSIGGARVEVEVGYERFVIKGGKKSNED
Position: 121-150
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P7
Chemical type: Peptide from protein
Sequence: IGYSIGGARVEVEVGYERFVIKGGKKSNED
Position: 121-150
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P8
Chemical type: Peptide from protein
Sequence: IKGGKKSNEDTASVFLLGKELAYHTARGQV
Position: 141-170
Source molecule (GenBank): AAA58953.1
Assay category: BCell
Result: Positive

Epitope: P8
Chemical type: Peptide from protein
Sequence: IKGGKKSNEDTASVFLLGKELAYHTARGQV
Position: 141-170
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P8
Chemical type: Peptide from protein
Sequence: IKGGKKSNEDTASVFLLGKELAYHTARGQV
Position: 141-170
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P9
Chemical type: Peptide from protein
Sequence: LAYHTARGQVDRLATALGKMTKSEAKKWG
Position: 161-189
Source molecule (GenBank): AAA58953.1
Assay category: BCell
Result: Positive

Epitope: P9
Chemical type: Peptide from protein
Sequence: LAYHTARGQVDRLATALGKMTKSEAKKWG
Position: 161-189
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P9
Chemical type: Peptide from protein
Sequence: LAYHTARGQVDRLATALGKMTKSEAKKWG
Position: 161-189
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P11
Chemical type: Peptide from protein
Sequence: STSVMLNACYDLLTDGIGVVPYACAGIGGN
Position: 293-322
Source molecule (GenBank): AAA58953.1
Assay category: BCell
Result: Positive

Epitope: P11
Chemical type: Peptide from protein
Sequence: STSVMLNACYDLLTDGIGVVPYACAGIGGN
Position: 293-322
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P11
Chemical type: Peptide from protein
Sequence: STSVMLNACYDLLTDGIGVVPYACAGIGGN
Position: 293-322
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P12
Chemical type: Peptide from protein
Sequence: PYACAGIGGNFVSVVDGHINPKFAYRVKAG
Position: 313-342
Source molecule (GenBank): AAA58953.1
Assay category: BCell
Result: Positive

Epitope: P12
Chemical type: Peptide from protein
Sequence: PYACAGIGGNFVSVVDGHINPKFAYRVKAG
Position: 313-342
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P12
Chemical type: Peptide from protein
Sequence: PYACAGIGGNFVSVVDGHINPKFAYRVKAG
Position: 313-342
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P13
Chemical type: Peptide from protein
Sequence: PKFAYRVKAGLSYALTPEISAFAGAFYHKV
Position: 333-362
Source molecule (GenBank): AAA58953.1
Assay category: BCell
Result: Negative

Epitope: P13
Chemical type: Peptide from protein
Sequence: PKFAYRVKAGLSYALTPEISAFAGAFYHKV
Position: 333-362
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P13
Chemical type: Peptide from protein
Sequence: PKFAYRVKAGLSYALTPEISAFAGAFYHKV
Position: 333-362
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P15
Chemical type: Peptide from protein
Sequence: LSHISDYTGTAGKNKDTGIASFNFAYFGGE
Position: 373-402
Source molecule (GenBank): AAA58953.1
Assay category: BCell
Result: Negative

Epitope: P15
Chemical type: Peptide from protein
Sequence: LSHISDYTGTAGKNKDTGIASFNFAYFGGE
Position: 373-402
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P15
Chemical type: Peptide from protein
Sequence: LSHISDYTGTAGKNKDTGIASFNFAYFGGE
Position: 373-402
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P16
Chemical type: Peptide from protein
Sequence: AGKNKDTGIASFNFAYFGGELGVRFAF
Position: 383-409
Source molecule (GenBank): AAA58953.1
Assay category: BCell
Result: Negative

Epitope: P16
Chemical type: Peptide from protein
Sequence: AGKNKDTGIASFNFAYFGGELGVRFAF
Position: 383-409
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: P16
Chemical type: Peptide from protein
Sequence: AGKNKDTGIASFNFAYFGGELGVRFAF
Position: 383-409
Source molecule (GenBank): AAA58953.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HV2
Chemical type: Peptide from protein
Sequence: DRLATALGKMTKGEAKKWGNAIESATGTTS
Position: 171-200
Source molecule (GenBank): AAN28952.1
Assay category: BCell
Result: Positive

Epitope: HV2
Chemical type: Peptide from protein
Sequence: DRLATALGKMTKGEAKKWGNAIESATGTTS
Position: 171-200
Source molecule (GenBank): AAN28952.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HV2
Chemical type: Peptide from protein
Sequence: DRLATALGKMTKGEAKKWGNAIESATGTTS
Position: 171-200
Source molecule (GenBank): AAN28952.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HV3
Chemical type: Peptide from protein
Sequence: TKGEAKKWGNAIESATGTTSGDELSKKVCG
Position: 181-210
Source molecule (GenBank): AAN28952.1
Assay category: BCell
Result: Positive

Epitope: HV3
Chemical type: Peptide from protein
Sequence: TKGEAKKWGNAIESATGTTSGDELSKKVCG
Position: 181-210
Source molecule (GenBank): AAN28952.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HV3
Chemical type: Peptide from protein
Sequence: TKGEAKKWGNAIESATGTTSGDELSKKVCG
Position: 181-210
Source molecule (GenBank): AAN28952.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HV4
Chemical type: Peptide from protein
Sequence: AIESATGTTSGDELSKKVCGKGTTSGSTN
Position: 191-219
Source molecule (GenBank): AAN28952.1
Assay category: BCell
Result: Positive

Epitope: HV4
Chemical type: Peptide from protein
Sequence: AIESATGTTSGDELSKKVCGKGTTSGSTN
Position: 191-219
Source molecule (GenBank): AAN28952.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HV4
Chemical type: Peptide from protein
Sequence: AIESATGTTSGDELSKKVCGKGTTSGSTN
Position: 191-219
Source molecule (GenBank): AAN28952.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HV5
Chemical type: Peptide from protein
Sequence: GDELSKKVCGKGTTSGSTNQCGTTDSTAT
Position: 201-229
Source molecule (GenBank): AAN28952.1
Assay category: BCell
Result: Positive

Epitope: HV5
Chemical type: Peptide from protein
Sequence: GDELSKKVCGKGTTSGSTNQCGTTDSTAT
Position: 201-229
Source molecule (GenBank): AAN28952.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HV5
Chemical type: Peptide from protein
Sequence: GDELSKKVCGKGTTSGSTNQCGTTDSTAT
Position: 201-229
Source molecule (GenBank): AAN28952.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HV6
Chemical type: Peptide from protein
Sequence: CGTTDSTATTKISEVFTE
Position: 221-238
Source molecule (GenBank): AAN28952.1
Assay category: BCell
Result: Positive

Epitope: HV6
Chemical type: Peptide from protein
Sequence: CGTTDSTATTKISEVFTE
Position: 221-238
Source molecule (GenBank): AAN28952.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HV6
Chemical type: Peptide from protein
Sequence: CGTTDSTATTKISEVFTE
Position: 221-238
Source molecule (GenBank): AAN28952.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HV7
Chemical type: Peptide from protein
Sequence: QCGTTDSTATTKISEVFTEGTDTLLSV
Position: 220-246
Source molecule (GenBank): AAN28952.1
Assay category: BCell
Result: Positive

Epitope: HV7
Chemical type: Peptide from protein
Sequence: QCGTTDSTATTKISEVFTEGTDTLLSV
Position: 220-246
Source molecule (GenBank): AAN28952.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HV7
Chemical type: Peptide from protein
Sequence: QCGTTDSTATTKISEVFTEGTDTLLSV
Position: 220-246
Source molecule (GenBank): AAN28952.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HV8
Chemical type: Peptide from protein
Sequence: TKISEVFTEGTDTLLSVEGNKDT
Position: 230-252
Source molecule (GenBank): AAN28952.1
Assay category: BCell
Result: Positive

Epitope: HV8
Chemical type: Peptide from protein
Sequence: TKISEVFTEGTDTLLSVEGNKDT
Position: 230-252
Source molecule (GenBank): AAN28952.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HV8
Chemical type: Peptide from protein
Sequence: TKISEVFTEGTDTLLSVEGNKDT
Position: 230-252
Source molecule (GenBank): AAN28952.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HV9
Chemical type: Peptide from protein
Sequence: GTDTLLSVEGNKDTINLQGMANNI
Position: 239-262
Source molecule (GenBank): AAN28952.1
Assay category: BCell
Result: Positive

Epitope: HV9
Chemical type: Peptide from protein
Sequence: GTDTLLSVEGNKDTINLQGMANNI
Position: 239-262
Source molecule (GenBank): AAN28952.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HV9
Chemical type: Peptide from protein
Sequence: GTDTLLSVEGNKDTINLQGMANNI
Position: 239-262
Source molecule (GenBank): AAN28952.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HV10
Chemical type: Peptide from protein
Sequence: EGNKDTINLQGMANNINNLSKEDKAV
Position: 247-272
Source molecule (GenBank): AAN28952.1
Assay category: BCell
Result: Positive

Epitope: HV10
Chemical type: Peptide from protein
Sequence: EGNKDTINLQGMANNINNLSKEDKAV
Position: 247-272
Source molecule (GenBank): AAN28952.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HV10
Chemical type: Peptide from protein
Sequence: EGNKDTINLQGMANNINNLSKEDKAV
Position: 247-272
Source molecule (GenBank): AAN28952.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HV11
Chemical type: Peptide from protein
Sequence: INLQGMANNINNLSKEDKAVVAGAFARAVE
Position: 253-282
Source molecule (GenBank): AAN28952.1
Assay category: BCell
Result: Positive

Epitope: HV11
Chemical type: Peptide from protein
Sequence: INLQGMANNINNLSKEDKAVVAGAFARAVE
Position: 253-282
Source molecule (GenBank): AAN28952.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HV11
Chemical type: Peptide from protein
Sequence: INLQGMANNINNLSKEDKAVVAGAFARAVE
Position: 253-282
Source molecule (GenBank): AAN28952.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HV12
Chemical type: Peptide from protein
Sequence: NNLSKEDKAVVAGAFARAVEGAEVIEVRAI
Position: 263-292
Source molecule (GenBank): AAN28952.1
Assay category: BCell
Result: Positive

Epitope: HV12
Chemical type: Peptide from protein
Sequence: NNLSKEDKAVVAGAFARAVEGAEVIEVRAI
Position: 263-292
Source molecule (GenBank): AAN28952.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HV12
Chemical type: Peptide from protein
Sequence: NNLSKEDKAVVAGAFARAVEGAEVIEVRAI
Position: 263-292
Source molecule (GenBank): AAN28952.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo