Reference: Shuming Chen; Yili Li; Florence R Depontieu; Tracee L McMiller; A Michelle English; Jeffrey Shabanowitz; Ferdynand Kos; John Sidney; Alessandro Sette; Steven A Rosenberg; Donald F Hunt; Roy A Mariuzza; Suzanne L Topalian. "Structure-based design of altered MHC class II-restricted peptide ligands with heterogeneous immunogenicity." Journal of immunology (Baltimore, Md. : 1950) (2013).

Epitope: gp100 (44-59)
Chemical type: Peptide from protein
Sequence: WNRQLYPEWTEAQRLD
Position: 44-59
Source molecule (GenBank): P40967.2
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: gp100 (44-59)
Chemical type: Peptide from protein
Sequence: WNRQLYPEWTEAQRLD
Position: 44-59
Source molecule (GenBank): P40967.2
Assay category: TCell
Result: Positive
Cell type: T cell
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: gp100 (44-59)
Chemical type: Peptide from protein
Sequence: WNRQLYPEWTEAQRLD
Position: 44-59
Source molecule (GenBank): P40967.2
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: gp100 (44-59)
Chemical type: Peptide from protein
Sequence: WNRQLYPEWTEAQRLD
Position: 44-59
Source molecule (GenBank): P40967.2
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: gp100 (44-59)
Chemical type: Peptide from protein
Sequence: WNRQLYPEWTEAQRLD
Position: 44-59
Source molecule (GenBank): P40967.2
Assay category: MhcLigandElution
Result: Positive

Epitope: gp100 (44-59)
Chemical type: Peptide from protein
Sequence: WNRQLYPEWTEAQRLD
Position: 44-59
Source molecule (GenBank): P40967.2
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 627.0

Epitope: gp100 (44-59)
Chemical type: Peptide from protein
Sequence: WNRQLYPEWTEAQRLD
Position: 44-59
Source molecule (GenBank): P40967.2
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 2.5

Epitope: L48F
Chemical type: Peptide, no natural source
Sequence: WNRQFYPEWTEAQRLD
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 280.0

Epitope: Y49M
Chemical type: Peptide, no natural source
Sequence: WNRQLMPEWTEAQRLD
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 521.0

Epitope: P50A
Chemical type: Peptide, no natural source
Sequence: WNRQLYAEWTEAQRLD
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 189.0

Epitope: E51A
Chemical type: Peptide, no natural source
Sequence: WNRQLYPAWTEAQRLD
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 199.0

Epitope: W52A
Chemical type: Peptide, no natural source
Sequence: WNRQLYPEATEAQRLD
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 372.0

Epitope: T53V
Chemical type: Peptide, no natural source
Sequence: WNRQLYPEWVEAQRLD
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 489.0

Epitope: E54L
Chemical type: Peptide, no natural source
Sequence: WNRQLYPEWTLAQRLD
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 65.0

Epitope: E54T
Chemical type: Peptide, no natural source
Sequence: WNRQLYPEWTTAQRLD
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 104.0

Epitope: A55G
Chemical type: Peptide, no natural source
Sequence: WNRQLYPEWTEGQRLD
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 553.0

Epitope: Q56I
Chemical type: Peptide, no natural source
Sequence: WNRQLYPEWTEAIRLD
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 926.0

Epitope: gp100 (44-57)
Chemical type: Peptide from protein
Sequence: WNRQLYPEWTEAQR
Position: 44-57
Source molecule (GenBank): P40967.2
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: gp100 (44-57)
Chemical type: Peptide from protein
Sequence: WNRQLYPEWTEAQR
Position: 44-57
Source molecule (GenBank): P40967.2
Assay category: MhcLigandElution
Result: Positive

Epitope: gp100 (45-57)
Chemical type: Peptide from protein
Sequence: NRQLYPEWTEAQR
Position: 45-57
Source molecule (GenBank): P40967.2
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: gp100 (45-57)
Chemical type: Peptide from protein
Sequence: NRQLYPEWTEAQR
Position: 45-57
Source molecule (GenBank): P40967.2
Assay category: MhcLigandElution
Result: Positive

Epitope: gp100 (45-59)
Chemical type: Peptide from protein
Sequence: NRQLYPEWTEAQRLD
Position: 45-59
Source molecule (GenBank): P40967.2
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: gp100 (45-59)
Chemical type: Peptide from protein
Sequence: NRQLYPEWTEAQRLD
Position: 45-59
Source molecule (GenBank): P40967.2
Assay category: MhcLigandElution
Result: Positive

Epitope: gp100 (46-57)
Chemical type: Peptide from protein
Sequence: RQLYPEWTEAQR
Position: 46-57
Source molecule (GenBank): P40967.2
Assay category: MhcLigandElution
Result: Positive

Epitope: gp100 (46-59)
Chemical type: Peptide from protein
Sequence: RQLYPEWTEAQRLD
Position: 46-59
Source molecule (GenBank): P40967.2
Assay category: MhcLigandElution
Result: Positive

Epitope: gp100 (40-57)
Chemical type: Peptide from protein
Sequence: RTKAWNRQLYPEWTEAQR
Position: 40-57
Source molecule (GenBank): P40967.2
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: gp100 (40-57)
Chemical type: Peptide from protein
Sequence: RTKAWNRQLYPEWTEAQR
Position: 40-57
Source molecule (GenBank): P40967.2
Assay category: MhcLigandElution
Result: Positive

Epitope: E51Q
Chemical type: Peptide, no natural source
Sequence: WNRQLYPQWTEAQRLD
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 200.0