Reference: Xiaoting Shen; Qinru Lin; Zhiming Liang; Jing Wang; Xinyi Yang; Yue Liang; Huitong Liang; Hanyu Pan; Jinlong Yang; Yuqi Zhu; Min Li; Weirong Xiang; Huanzhang Zhu. "Reduction of Pre-Existing Adaptive Immune Responses Against SaCas9 in Humans Using Epitope Mapping and Identification." The CRISPR journal (2022).

Epitope: D79
Chemical type: Discontinuous protein residues
Discontinuous residues: D79
Source molecule (GenBank): J7RUA5.1
Assay category: BCell
Result: Positive-Low

Epitope: A144
Chemical type: Discontinuous protein residues
Discontinuous residues: A144
Source molecule (GenBank): J7RUA5.1
Assay category: BCell
Result: Positive-Low

Epitope: E146
Chemical type: Discontinuous protein residues
Discontinuous residues: E146
Source molecule (GenBank): J7RUA5.1
Assay category: BCell
Result: Positive-Low

Epitope: E147
Chemical type: Discontinuous protein residues
Discontinuous residues: E147
Source molecule (GenBank): J7RUA5.1
Assay category: BCell
Result: Positive-Low

Epitope: D161
Chemical type: Discontinuous protein residues
Discontinuous residues: D161
Source molecule (GenBank): J7RUA5.1
Assay category: BCell
Result: Positive-Low

Epitope: G162
Chemical type: Discontinuous protein residues
Discontinuous residues: G162
Source molecule (GenBank): J7RUA5.1
Assay category: BCell
Result: Positive-Low

Epitope: D270
Chemical type: Discontinuous protein residues
Discontinuous residues: D270
Source molecule (GenBank): J7RUA5.1
Assay category: BCell
Result: Positive-Low

Epitope: E271
Chemical type: Discontinuous protein residues
Discontinuous residues: E271
Source molecule (GenBank): J7RUA5.1
Assay category: BCell
Result: Positive-Intermediate

Epitope: N272
Chemical type: Discontinuous protein residues
Discontinuous residues: N272
Source molecule (GenBank): J7RUA5.1
Assay category: BCell
Result: Positive-Low

Epitope: E273
Chemical type: Discontinuous protein residues
Discontinuous residues: E273
Source molecule (GenBank): J7RUA5.1
Assay category: BCell
Result: Positive-Low

Epitope: P294
Chemical type: Discontinuous protein residues
Discontinuous residues: P294
Source molecule (GenBank): J7RUA5.1
Assay category: BCell
Result: Positive-Low

Epitope: T295
Chemical type: Discontinuous protein residues
Discontinuous residues: T295
Source molecule (GenBank): J7RUA5.1
Assay category: BCell
Result: Positive-Low

Epitope: D309
Chemical type: Discontinuous protein residues
Discontinuous residues: D309
Source molecule (GenBank): J7RUA5.1
Assay category: BCell
Result: Positive-Low

Epitope: E322
Chemical type: Discontinuous protein residues
Discontinuous residues: E322
Source molecule (GenBank): J7RUA5.1
Assay category: BCell
Result: Negative

Epitope: A337
Chemical type: Discontinuous protein residues
Discontinuous residues: A337
Source molecule (GenBank): J7RUA5.1
Assay category: BCell
Result: Negative

Epitope: R338
Chemical type: Discontinuous protein residues
Discontinuous residues: R338
Source molecule (GenBank): J7RUA5.1
Assay category: BCell
Result: Positive-High

Epitope: T369
Chemical type: Discontinuous protein residues
Discontinuous residues: T369
Source molecule (GenBank): J7RUA5.1
Assay category: BCell
Result: Positive-Low

Epitope: E378
Chemical type: Discontinuous protein residues
Discontinuous residues: E378
Source molecule (GenBank): J7RUA5.1
Assay category: BCell
Result: Negative

Epitope: SaCas9 200-208
Chemical type: Peptide from protein
Sequence: TYIDLLETR
Position: 200-208
Source molecule (GenBank): J7RUA5.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo