Reference: Neda Feizi; Chiara Focaccetti; Ilenia Pacella; Gloria Tucci; Alessandra Rossi; Massimo Costanza; Rosetta Pedotti; John Sidney; Alessandro Sette; Claudia La Rocca; Claudio Procaccini; Giuseppe Matarese; Vincenzo Barnaba; Silvia Piconese. "CD8<sup>+</sup> T cells specific for cryptic apoptosis-associated epitopes exacerbate experimental autoimmune encephalomyelitis." Cell death & disease (2021).

Epitope: MOG 35-55
Chemical type: Peptide from protein
Sequence: MEVGWYRSPFSRVVHLYRNGK
Position: 35-55
Source molecule (GenBank): AAA03180.1
Assay category: TCell
Result: Positive
Host: F 8-12 weeks
Cell type: lymphocyte
Tissue: central nervous system (CNS)
Culture conditions: Direct Ex Vivo

Epitope: VIM 66-74
Chemical type: Peptide from protein
Sequence: SAVRLRSSV
Position: 66-74
Source molecule (GenBank): P20152.3
Assay category: TCell
Result: Positive
Host: F 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: VIM 66-74
Chemical type: Peptide from protein
Sequence: SAVRLRSSV
Position: 66-74
Source molecule (GenBank): P20152.3
Assay category: TCell
Result: Positive
Host: F 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: VIM 66-74
Chemical type: Peptide from protein
Sequence: SAVRLRSSV
Position: 66-74
Source molecule (GenBank): P20152.3
Assay category: TCell
Result: Positive
Host: F 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: VIM 367-376
Chemical type: Peptide from protein
Sequence: DEIQNMKEEM
Position: 367-376
Source molecule (GenBank): P20152.3
Assay category: TCell
Result: Positive
Host: F 8-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: VIM 353-362
Chemical type: Peptide from protein
Sequence: LEAANYQDTI
Position: 353-362
Source molecule (GenBank): P20152.3
Assay category: TCell
Result: Positive
Host: F 8-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: MYH9 120-127
Chemical type: Peptide from protein
Sequence: VINPYKNL
Position: 120-127
Source molecule (GenBank): Q8VDD5.4
Assay category: TCell
Result: Positive
Host: F 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: MYH9 120-127
Chemical type: Peptide from protein
Sequence: VINPYKNL
Position: 120-127
Source molecule (GenBank): Q8VDD5.4
Assay category: TCell
Result: Positive
Host: F 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: MYH9 120-127
Chemical type: Peptide from protein
Sequence: VINPYKNL
Position: 120-127
Source molecule (GenBank): Q8VDD5.4
Assay category: TCell
Result: Positive
Host: F 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: MYH9 761-768
Chemical type: Peptide from protein
Sequence: VFFRAGVL
Position: 761-768
Source molecule (GenBank): Q8VDD5.4
Assay category: TCell
Result: Positive
Host: F 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: MYH9 761-768
Chemical type: Peptide from protein
Sequence: VFFRAGVL
Position: 761-768
Source molecule (GenBank): Q8VDD5.4
Assay category: TCell
Result: Positive
Host: F 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: MYH9 761-768
Chemical type: Peptide from protein
Sequence: VFFRAGVL
Position: 761-768
Source molecule (GenBank): Q8VDD5.4
Assay category: TCell
Result: Positive
Host: F 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: MYH9 761-768
Chemical type: Peptide from protein
Sequence: VFFRAGVL
Position: 761-768
Source molecule (GenBank): Q8VDD5.4
Assay category: TCell
Result: Positive
Host: F 8-12 weeks
Cell type: T cell CD8+
Tissue: central nervous system (CNS)
Culture conditions: Direct Ex Vivo

Epitope: MYH9 761-768
Chemical type: Peptide from protein
Sequence: VFFRAGVL
Position: 761-768
Source molecule (GenBank): Q8VDD5.4
Assay category: TCell
Result: Negative
Host: F 7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: MYH9 761-768
Chemical type: Peptide from protein
Sequence: VFFRAGVL
Position: 761-768
Source molecule (GenBank): Q8VDD5.4
Assay category: TCell
Result: Positive
Host: F 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: MYH9 761-768
Chemical type: Peptide from protein
Sequence: VFFRAGVL
Position: 761-768
Source molecule (GenBank): Q8VDD5.4
Assay category: TCell
Result: Positive
Host: F 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: MYH9 761-768
Chemical type: Peptide from protein
Sequence: VFFRAGVL
Position: 761-768
Source molecule (GenBank): Q8VDD5.4
Assay category: TCell
Result: Positive
Host: F 8-12 weeks

Epitope: MYH9 761-768
Chemical type: Peptide from protein
Sequence: VFFRAGVL
Position: 761-768
Source molecule (GenBank): Q8VDD5.4
Assay category: TCell
Result: Positive-Low
Host: F 7 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo