Reference: Laura Romero-Castillo; Rajan Kumar Pandey; Bingze Xu; Christian M Beusch; Ana Oliveira-Coelho; Kejsi Zeqiraj; Carolin Svensson; Zhongwei Xu; Huqiao Luo; Outi Sareila; Pierre Sabatier; Changrong Ge; Lei Cheng; Vilma Urbonaviciute; Alexander Krämer; Cecilia Lindgren; Sabrina Haag; Johan Viljanen; Roman A Zubarev; Jan Kihlberg; Anna Linusson; Harald Burkhardt; Rikard Holmdahl. "Tolerogenic antigen-specific vaccine induces VISTA-enriched regulatory T cells and protects against arthritis in DRB1∗04:01 mice." Molecular therapy : the journal of the American Society of Gene Therapy (2025).

Epitope: GalCOL2-AL170-AL177
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGET
Position: 391-405
Source molecule (GenBank): P05539.2
Assay category: TCell
Result: Positive
Cell type: T cell hybridoma
Tissue: lymph node
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: GalCOL2-AL170-AL177
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGET
Position: 391-405
Source molecule (GenBank): P05539.2
Assay category: MhcBinding
Result: Positive

Epitope: GalCOL2-AL178
Chemical type: Peptide, no natural source
Sequence: GIAGFKMEQGPKGET
Assay category: MhcBinding
Result: Positive

Epitope: GalCOL2-AL179
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGET
Position: 391-405
Source molecule (GenBank): P05539.2
Assay category: TCell
Result: Positive
Cell type: T cell hybridoma
Tissue: lymph node
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: GalCOL2-AL179
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGET
Position: 391-405
Source molecule (GenBank): P05539.2
Assay category: MhcBinding
Result: Positive

Epitope: COL2 259-273
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGET
Position: 391-405
Source molecule (GenBank): P05539.2
Assay category: TCell
Result: Positive-Intermediate
Host:  8-10 weeks
Cell type: lymphocyte
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: COL2 259-273
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGET
Position: 391-405
Source molecule (GenBank): P05539.2
Assay category: TCell
Result: Positive-High
Host:  12-14 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: COL2 259-273
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGET
Position: 391-405
Source molecule (GenBank): P05539.2
Assay category: TCell
Result: Positive-Intermediate
Host:  8-10 weeks

Epitope: COL2 259-273
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGET
Position: 391-405
Source molecule (GenBank): P05539.2
Assay category: TCell
Result: Negative
Host:  12-14 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: COL2 259-273
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGET
Position: 391-405
Source molecule (GenBank): P05539.2
Assay category: TCell
Result: Negative
Host:  12-14 weeks

Epitope: COL2 259-273
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGET
Position: 391-405
Source molecule (GenBank): P05539.2
Assay category: TCell
Result: Positive-Low
Host:  12-14 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: COL2 259-273
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGET
Position: 391-405
Source molecule (GenBank): P05539.2
Assay category: MhcBinding
Result: Positive

Epitope: COL2-AL170/AL177
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGET
Position: 391-405
Source molecule (GenBank): P05539.2
Assay category: TCell
Result: Positive-Intermediate
Host:  8-10 weeks

Epitope: COL2-AL178
Chemical type: Peptide, no natural source
Sequence: GIAGFKMEQGPKGET
Assay category: TCell
Result: Negative
Host:  8-10 weeks

Epitope: COL2-AL179
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGET
Position: 391-405
Source molecule (GenBank): P05539.2
Assay category: TCell
Result: Positive-High
Host:  8-10 weeks

Epitope: COL2-AL179
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGET
Position: 391-405
Source molecule (GenBank): P05539.2
Assay category: TCell
Result: Positive-Low
Host:  12-14 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: COL2-AL179
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGET
Position: 391-405
Source molecule (GenBank): P05539.2
Assay category: TCell
Result: Positive
Host:  12-14 weeks

Epitope: COL2-AL179
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGET
Position: 391-405
Source molecule (GenBank): P05539.2
Assay category: TCell
Result: Positive
Host:  12-14 weeks

Epitope: COL2-AL179
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGET
Position: 391-405
Source molecule (GenBank): P05539.2
Assay category: TCell
Result: Positive-High
Host:  8-10 weeks
Cell type: lymphocyte
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: COL2-AL179
Chemical type: Peptide from protein
Sequence: GIAGFKGEQGPKGET
Position: 391-405
Source molecule (GenBank): P05539.2
Assay category: MhcBinding
Result: Positive

Epitope: U1-R-R
Chemical type: Peptide from protein
Sequence: GDQGVPGEAGAPGLVGPRGERGFP
Position: 681-704
Source molecule (GenBank): P02459.4
Assay category: BCell
Result: Positive
Host:  12-14 weeks

Epitope: U1-R-R
Chemical type: Peptide from protein
Sequence: GDQGVPGEAGAPGLVGPRGERGFP
Position: 681-704
Source molecule (GenBank): P02459.4
Assay category: BCell
Result: Positive
Host:  12-14 weeks

Epitope: C1-R-R
Chemical type: Peptide from protein
Sequence: GDPGRPGEPGLPGARGLTGRPGDA
Position: 546-569
Source molecule (GenBank): P02459.4
Assay category: BCell
Result: Positive
Host:  12-14 weeks

Epitope: C1-R-R
Chemical type: Peptide from protein
Sequence: GDPGRPGEPGLPGARGLTGRPGDA
Position: 546-569
Source molecule (GenBank): P02459.4
Assay category: BCell
Result: Positive
Host:  12-14 weeks

Epitope: C1-cit-R
Chemical type: Peptide from protein
Sequence: GDPGRPGEPGLPGARGLTGRPGDA
Position: 546-569
Source molecule (GenBank): P02459.4
Assay category: BCell
Result: Positive
Host:  12-14 weeks

Epitope: C1-cit-R
Chemical type: Peptide from protein
Sequence: GDPGRPGEPGLPGARGLTGRPGDA
Position: 546-569
Source molecule (GenBank): P02459.4
Assay category: BCell
Result: Positive
Host:  12-14 weeks

Epitope: U1-R-cit
Chemical type: Peptide from protein
Sequence: GDQGVPGEAGAPGLVGPRGERGFP
Position: 681-704
Source molecule (GenBank): P02459.4
Assay category: BCell
Result: Positive
Host:  12-14 weeks

Epitope: U1-R-cit
Chemical type: Peptide from protein
Sequence: GDQGVPGEAGAPGLVGPRGERGFP
Position: 681-704
Source molecule (GenBank): P02459.4
Assay category: BCell
Result: Positive
Host:  12-14 weeks

Epitope: J1-R
Chemical type: Peptide from protein
Sequence: GAQGPPGLQGMPGERGAAGIAGPK
Position: 741-764
Source molecule (GenBank): P02459.4
Assay category: BCell
Result: Positive
Host:  12-14 weeks

Epitope: J1-R
Chemical type: Peptide from protein
Sequence: GAQGPPGLQGMPGERGAAGIAGPK
Position: 741-764
Source molecule (GenBank): P02459.4
Assay category: BCell
Result: Positive
Host:  12-14 weeks

Epitope: D3-R
Chemical type: Peptide from protein
Sequence: GPTGVTGPKGARGAQGPPGATGFP
Position: 876-899
Source molecule (GenBank): P02459.4
Assay category: BCell
Result: Positive
Host:  12-14 weeks

Epitope: D3-R
Chemical type: Peptide from protein
Sequence: GPTGVTGPKGARGAQGPPGATGFP
Position: 876-899
Source molecule (GenBank): P02459.4
Assay category: BCell
Result: Positive
Host:  12-14 weeks

Epitope: E10-R
Chemical type: Peptide from protein
Sequence: GAEGPPGPQGLAGQRGIVGLPGQR
Position: 966-989
Source molecule (GenBank): P02458.3
Assay category: BCell
Result: Positive
Host:  12-14 weeks

Epitope: E10-R
Chemical type: Peptide from protein
Sequence: GAEGPPGPQGLAGQRGIVGLPGQR
Position: 966-989
Source molecule (GenBank): P02458.3
Assay category: BCell
Result: Positive
Host:  12-14 weeks

Epitope: F4-R-R
Chemical type: Peptide from protein
Sequence: GDKGEAGEPGERGLKGHRGFTGLQ
Position: 1116-1139
Source molecule (GenBank): P02458.3
Assay category: BCell
Result: Positive
Host:  12-14 weeks

Epitope: F4-R-R
Chemical type: Peptide from protein
Sequence: GDKGEAGEPGERGLKGHRGFTGLQ
Position: 1116-1139
Source molecule (GenBank): P02458.3
Assay category: BCell
Result: Negative
Host:  12-14 weeks

Epitope: F4-R-cit
Chemical type: Peptide from protein
Sequence: GDKGEAGEPGERGLKGHRGFTGLQ
Position: 1116-1139
Source molecule (GenBank): P02458.3
Assay category: BCell
Result: Positive
Host:  12-14 weeks

Epitope: F4-R-cit
Chemical type: Peptide from protein
Sequence: GDKGEAGEPGERGLKGHRGFTGLQ
Position: 1116-1139
Source molecule (GenBank): P02458.3
Assay category: BCell
Result: Negative
Host:  12-14 weeks