Reference: Shayesteh R Ferdosi; Radwa Ewaisha; Farzaneh Moghadam; Sri Krishna; Jin G Park; Mo R Ebrahimkhani; Samira Kiani; Karen S Anderson. "Multifunctional CRISPR-Cas9 with engineered immunosilenced human T cell epitopes." Nature communications (2019).

Epitope: 1
Chemical type: Peptide from protein
Sequence: YLNAVVGTAL
Position: 987-996
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 2
Chemical type: Peptide from protein
Sequence: ILADANLDKV
Position: 1281-1290
Source molecule (GenBank): WP_032460140.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 3
Chemical type: Peptide from protein
Sequence: GLFGNLIAL
Position: 236-244
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 4
Chemical type: Peptide from protein
Sequence: NLIALSLGL
Position: 240-248
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: 4
Chemical type: Peptide from protein
Sequence: NLIALSLGL
Position: 240-248
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 5
Chemical type: Peptide from protein
Sequence: ILEDIVLTL
Position: 615-623
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: 5
Chemical type: Peptide from protein
Sequence: ILEDIVLTL
Position: 615-623
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 6
Chemical type: Peptide from protein
Sequence: DILEDIVLTL
Position: 614-623
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 7
Chemical type: Peptide from protein
Sequence: SLHEHIANL
Position: 719-727
Source molecule (GenBank): WP_032460140.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 8
Chemical type: Peptide from protein
Sequence: HLGELHAIL
Position: 415-423
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 9
Chemical type: Peptide from protein
Sequence: ILLSDILRV
Position: 300-308
Source molecule (GenBank): WP_032460140.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 10
Chemical type: Peptide from protein
Sequence: VLSMPQVNIV
Position: 1085-1094
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 11
Chemical type: Peptide from protein
Sequence: SLHEHIANLA
Position: 719-728
Source molecule (GenBank): WP_032460140.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 12
Chemical type: Peptide from protein
Sequence: LIIKLPKYSL
Position: 1194-1203
Source molecule (GenBank): WP_032460140.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 13
Chemical type: Peptide from protein
Sequence: TLIHQSITGL
Position: 1349-1358
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 14
Chemical type: Peptide from protein
Sequence: KLPKYSLFEL
Position: 1197-1206
Source molecule (GenBank): WP_032460140.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 15
Chemical type: Peptide from protein
Sequence: NIMNFFKTEI
Position: 1040-1049
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 16
Chemical type: Peptide from protein
Sequence: SLLYEYFTV
Position: 512-520
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 17
Chemical type: Peptide from protein
Sequence: IIHLFTLTNL
Position: 1309-1318
Source molecule (GenBank): WP_032460140.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 18
Chemical type: Peptide from protein
Sequence: RLSRKLINGI
Position: 661-670
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 19
Chemical type: Peptide from protein
Sequence: ALPSKYVNFL
Position: 1227-1236
Source molecule (GenBank): WP_032460140.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 20
Chemical type: Peptide from protein
Sequence: ALIKKYPKL
Position: 995-1003
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 21
Chemical type: Peptide from protein
Sequence: RLENLIAQL
Position: 221-229
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 22
Chemical type: Peptide from protein
Sequence: YLASHYEKL
Position: 1237-1245
Source molecule (GenBank): WP_032460140.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 24
Chemical type: Peptide from protein
Sequence: IMNFFKTEI
Position: 1041-1049
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 25
Chemical type: Peptide from protein
Sequence: YLQNGRDMYV
Position: 814-823
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 26
Chemical type: Peptide from protein
Sequence: RMLASAGEL
Position: 1212-1220
Source molecule (GenBank): WP_032460140.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 27
Chemical type: Peptide from protein
Sequence: KMIAKSEQEI
Position: 1019-1028
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 28
Chemical type: Peptide from protein
Sequence: SQILKEHPV
Position: 792-800
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 29
Chemical type: Peptide from protein
Sequence: KVVDELVKV
Position: 742-750
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 30
Chemical type: Peptide from protein
Sequence: FLEAKGYKEV
Position: 1181-1190
Source molecule (GenBank): WP_032460140.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 31
Chemical type: Peptide from protein
Sequence: HMIKFRGHFL
Position: 160-169
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 32
Chemical type: Peptide from protein
Sequence: LLFKTNRKV
Position: 551-559
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 33
Chemical type: Peptide from protein
Sequence: KLVDSTDKA
Position: 141-149
Source molecule (GenBank): WP_136093366.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 34
Chemical type: Peptide from protein
Sequence: TITPWNFEEV
Position: 472-481
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 35
Chemical type: Peptide from protein
Sequence: QLFEENPINA
Position: 194-203
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 36
Chemical type: Peptide from protein
Sequence: FTVYNELTKV
Position: 518-527
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 37
Chemical type: Peptide from protein
Sequence: ITPWNFEEV
Position: 473-481
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 38
Chemical type: Peptide from protein
Sequence: FQFYKVREI
Position: 969-977
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: β2
Chemical type: Peptide, no natural source
Sequence: IGEDIVLTL
Assay category: TCell
Result: Positive-Low
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: β2
Chemical type: Peptide, no natural source
Sequence: IGEDIVLTL
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: β9
Chemical type: Peptide, no natural source
Sequence: ILEDIVLTG
Assay category: TCell
Result: Positive-Low
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: β29
Chemical type: Peptide, no natural source
Sequence: IGEDIVLTG
Assay category: TCell
Result: Positive-Low
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α2
Chemical type: Peptide, no natural source
Sequence: NGIALSLGL
Assay category: TCell
Result: Positive-Low
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α2
Chemical type: Peptide, no natural source
Sequence: NGIALSLGL
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: α9
Chemical type: Peptide, no natural source
Sequence: NLIALSLGG
Assay category: TCell
Result: Positive-Low
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α29
Chemical type: Peptide, no natural source
Sequence: NGIALSLGG
Assay category: TCell
Result: Positive-Low
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 16
Chemical type: Peptide from protein
Sequence: FLYLASHYEK
Position: 1235-1244
Source molecule (GenBank): WP_032460140.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 20
Chemical type: Peptide from protein
Sequence: LSMPQVNIVK
Position: 1086-1095
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 22
Chemical type: Peptide from protein
Sequence: YSNIMNFFK
Position: 1038-1046
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 23
Chemical type: Peptide from protein
Sequence: KYPKLESEF
Position: 999-1007
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 24
Chemical type: Peptide from protein
Sequence: RYTRRKNRI
Position: 71-79
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 25
Chemical type: Peptide from protein
Sequence: YFFYSNIMNF
Position: 1036-1045
Source molecule (GenBank): WP_032460140.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 1
Chemical type: Peptide from protein
Sequence: ILEDIVLTLTLFEDR
Position: 615-629
Source molecule (GenBank): WP_032460140.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 2
Chemical type: Peptide from protein
Sequence: KNGLFGNLIALSLGLTPNF
Position: 234-252
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 3
Chemical type: Peptide from protein
Sequence: RLSDYDVAAIVPQSFLK
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 4
Chemical type: Peptide from protein
Sequence: ERHPIFGNIVDEVAYHY
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 5
Chemical type: Peptide from protein
Sequence: VLPKHSLLYEYFTVYNELT
Position: 507-525
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 6
Chemical type: Peptide from protein
Sequence: ATAKYFFYSNIMNFFK
Position: 1032-1047
Source molecule (GenBank): WP_032460140.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 7
Chemical type: Peptide from protein
Sequence: DLRLIYLALAHMIKFR
Position: 150-165
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 8
Chemical type: Peptide from protein
Sequence: MKNYWRQLLNAKLITQ
Position: 878-893
Source molecule (GenBank): WP_011528583.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo