Reference: Helen Mokhtar; Miriam Pedrera; Jean-Pierre Frossard; Lucia Biffar; Sabine E Hammer; Lise K Kvisgaard; Lars E Larsen; Graham R Stewart; Satyanarayana Somavarapu; Falko Steinbach; Simon P Graham. "The Non-structural Protein 5 and Matrix Protein Are Antigenic Targets of T Cell Immunity to Genotype 1 Porcine Reproductive and Respiratory Syndrome Viruses." Frontiers in immunology (2016).

Epitope: NSP5 (13-27)
Chemical type: Peptide from protein
Sequence: FLLWRMMGHAWTPIV
Position: 1892-1906
Source molecule (GenBank): ABF66340.1
Assay category: TCell
Result: Positive
Host:  12 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NSP5 (13-27)
Chemical type: Peptide from protein
Sequence: FLLWRMMGHAWTPIV
Position: 1892-1906
Source molecule (GenBank): ABF66340.1
Assay category: TCell
Result: Positive
Host:  12 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NSP5 (149-159)
Chemical type: Peptide from protein
Sequence: LHNMLVGDGSF
Position: 2028-2038
Source molecule (GenBank): ABF66340.1
Assay category: TCell
Result: Negative
Host:  12 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NSP5 (149-159)
Chemical type: Peptide from protein
Sequence: LHNMLVGDGSF
Position: 2028-2038
Source molecule (GenBank): ABF66340.1
Assay category: TCell
Result: Positive
Host:  12 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NSP5 (156–167)
Chemical type: Peptide from protein
Sequence: DGSFSSAFFLRY
Position: 2035-2046
Source molecule (GenBank): ABF66340.1
Assay category: TCell
Result: Positive
Host:  12 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NSP5 (156–167)
Chemical type: Peptide from protein
Sequence: DGSFSSAFFLRY
Position: 2035-2046
Source molecule (GenBank): ABF66340.1
Assay category: TCell
Result: Positive
Host:  12 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: M (29-43)
Chemical type: Peptide from protein
Sequence: MIYALKVSRGRLLGL
Position: 30-44
Source molecule (GenBank): ADD69930.1
Assay category: TCell
Result: Positive
Host:  12 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: M (29-43)
Chemical type: Peptide from protein
Sequence: MIYALKVSRGRLLGL
Position: 30-44
Source molecule (GenBank): ADD69930.1
Assay category: TCell
Result: Positive
Host:  12 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: M (33-47)
Chemical type: Peptide from protein
Sequence: LKVSRGRLLGLLHIL
Position: 33-47
Source molecule (GenBank): UIE29966.1
Assay category: TCell
Result: Positive
Host:  12 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: M (33-47)
Chemical type: Peptide from protein
Sequence: LKVSRGRLLGLLHIL
Position: 33-47
Source molecule (GenBank): UIE29966.1
Assay category: TCell
Result: Negative
Host:  12 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PRRSV Cresa 3262 M (29-43)
Chemical type: Peptide from protein
Sequence: MIYALKVSHGRLLGL
Position: 29-43
Source molecule (GenBank): ADX07047.1
Assay category: TCell
Result: Positive
Host:  12 weeks
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PRRSV BJEU06 M (13-27)
Chemical type: Peptide from protein
Sequence: FLLWRMMGYAWTPIV
Position: 1904-1918
Source molecule (GenBank): ACY56789.1
Assay category: TCell
Result: Positive
Host:  12 weeks
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PRRSV Cresa3262 M (13-27)
Chemical type: Peptide from protein
Sequence: FLLWRMMGHAWTPLV
Position: 1835-1849
Source molecule (GenBank): ADX07034.1
Assay category: TCell
Result: Positive
Host:  12 weeks
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PRRSV HKEU16 M (13-27)
Chemical type: Peptide from protein
Sequence: FLLWRMMGHAWTPVV
Position: 1909-1923
Source molecule (GenBank): ABU49667.1
Assay category: TCell
Result: Positive
Host:  12 weeks
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PRRSV NMEU09 M (13-27)
Chemical type: Peptide from protein
Sequence: FLLWRMMGHAWTPII
Position: 1907-1921
Source molecule (GenBank): ACY56797.1
Assay category: TCell
Result: Positive
Host:  12 weeks
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NSP5 (145-159)
Chemical type: Peptide from protein
Sequence: KYRCLHNMLVGDGSF
Position: 2024-2038
Source molecule (GenBank): ABF66340.1
Assay category: TCell
Result: Positive
Host:  12 weeks
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NSP5 HKEU16 (145-159)
Chemical type: Peptide from protein
Sequence: KHRCLHNMLVGDGSF
Position: 2041-2055
Source molecule (GenBank): ABU49667.1
Assay category: TCell
Result: Positive
Host:  12 weeks
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NSP5 NMEU09 (145-159)
Chemical type: Peptide from protein
Sequence: KHRCLHNILVGDGCF
Position: 2039-2053
Source molecule (GenBank): ACY56797.1
Assay category: TCell
Result: Positive
Host:  12 weeks
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NSP5 strain (145-159)
Chemical type: Peptide from protein
Sequence: KHRSLHNMLVGDGSF
Position: 1991-2005
Source molecule (GenBank): AKA60701.1
Assay category: TCell
Result: Positive
Host:  12 weeks
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NSP5 consensus (145-159)
Chemical type: Peptide from protein
Sequence: KYRCLHNVLVGDGVF
Position: 2128-2142
Source molecule (GenBank): ABR26248.1
Assay category: TCell
Result: Positive-Low
Host:  12 weeks
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NSP5 (149-163)
Chemical type: Peptide from protein
Sequence: LHNMLVGDGSFSSAF
Position: 2028-2042
Source molecule (GenBank): ABF66340.1
Assay category: TCell
Result: Positive
Host:  12 weeks
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NSP5 NMEU09 (149-163)
Chemical type: Peptide from protein
Sequence: LHNILVGDGCFSSAF
Position: 2043-2057
Source molecule (GenBank): ACY56797.1
Assay category: TCell
Result: Positive-Low
Host:  12 weeks
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NSP5 (149-163)
Chemical type: Peptide from protein
Sequence: LHNVLVGDGVFSSAF
Position: 2132-2146
Source molecule (GenBank): ABR26248.1
Assay category: TCell
Result: Positive-Low
Host:  12 weeks
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NSP5 (153-167)
Chemical type: Peptide from protein
Sequence: LVGDGSFSSAFFLRY
Position: 2032-2046
Source molecule (GenBank): ABF66340.1
Assay category: TCell
Result: Positive
Host:  12 weeks
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NSP5 NMEU09 (153-167)
Chemical type: Peptide from protein
Sequence: LVGDGCFSSAFFLRY
Position: 2047-2061
Source molecule (GenBank): ACY56797.1
Assay category: TCell
Result: Positive-Low
Host:  12 weeks
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NSP5 consensus (153-167)
Chemical type: Peptide from protein
Sequence: LVGDGVFSSAFFLRY
Position: 2136-2150
Source molecule (GenBank): ABR26248.1
Assay category: TCell
Result: Positive-Low
Host:  12 weeks
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NSP5 (156-170)
Chemical type: Peptide from protein
Sequence: GSFSSAFFLRYFAE
Position: 2036-2049
Source molecule (GenBank): ABF66340.1
Assay category: TCell
Result: Positive
Host:  12 weeks
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NSP5 NMEU09 (156-170)
Chemical type: Peptide from protein
Sequence: GCFSSAFFLRYFAE
Position: 2051-2064
Source molecule (GenBank): ACY56797.1
Assay category: TCell
Result: Positive-Low
Host:  12 weeks
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NSP5 consensus (156-170)
Chemical type: Peptide from protein
Sequence: GVFSSAFFLRYFAE
Position: 2140-2153
Source molecule (GenBank): ABR26248.1
Assay category: TCell
Result: Positive-Low
Host:  12 weeks
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo