Reference: Hakimeh Ebrahimi-Nik; Marmar Moussa; Ryan P Englander; Summit Singhaviranon; Justine Michaux; HuiSong Pak; Hiroko Miyadera; William L Corwin; Grant L J Keller; Adam T Hagymasi; Tatiana V Shcheglova; George Coukos; Brian M Baker; Ion I Mandoiu; Michal Bassani-Sternberg; Pramod K Srivastava. "Reversion analysis reveals the in vivo immunogenicity of a poorly MHC I-binding cancer neoepitope." Nature communications (2021).

Epitope: Ccdc85c MUT (18mer)
Chemical type: Peptide, no natural source
Sequence: DPSSTYIRPFETKVKLLD
Assay category: TCell
Result: Positive
Host: F 6-8 weeks

Epitope: Ccdc85c MUT (18mer)
Chemical type: Peptide, no natural source
Sequence: DPSSTYIRPFETKVKLLD
Assay category: TCell
Result: Positive-Low
Host: F 6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ccdc85c MUT (10mer)
Chemical type: Peptide, no natural source
Sequence: YIRPFETKVK
Assay category: TCell
Result: Positive
Host: F 6-8 weeks

Epitope: Ccdc85c MUT (10mer)
Chemical type: Peptide, no natural source
Sequence: YIRPFETKVK
Assay category: MhcLigandElution
Result: Positive

Epitope: Ccdc85c MUT (10mer)
Chemical type: Peptide, no natural source
Sequence: YIRPFETKVK
Assay category: MhcBinding
Result: Negative

Epitope: Ccdc85c MUT (11mer)
Chemical type: Peptide, no natural source
Sequence: TYIRPFETKVK
Assay category: TCell
Result: Positive
Host: F 6-8 weeks

Epitope: Ccdc85c MUT (11mer)
Chemical type: Peptide, no natural source
Sequence: TYIRPFETKVK
Assay category: MhcLigandElution
Result: Positive

Epitope: Ccdc85c MUT (11mer)
Chemical type: Peptide, no natural source
Sequence: TYIRPFETKVK
Assay category: MhcBinding
Result: Negative

Epitope: Ccdc85c WT
Chemical type: Peptide from protein
Sequence: DPSSTYIRPLETKVKLLD
Position: 266-283
Source molecule (GenBank): E9Q6B2.1
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ccdc85c WT
Chemical type: Peptide from protein
Sequence: DPSSTYIRPLETKVKLLD
Position: 266-283
Source molecule (GenBank): E9Q6B2.1
Assay category: TCell
Result: Negative
Host: F 6-8 weeks

Epitope: M1
Chemical type: Peptide, no natural source
Sequence: GTYIRPFETKVK
Assay category: MhcBinding
Result: Positive-Low

Epitope: M1
Chemical type: Peptide, no natural source
Sequence: GTYIRPFETKVK
Assay category: MhcBinding
Result: Negative

Epitope: M2
Chemical type: Peptide, no natural source
Sequence: GGYIRPFETKVK
Assay category: MhcBinding
Result: Negative

Epitope: M2
Chemical type: Peptide, no natural source
Sequence: GGYIRPFETKVK
Assay category: MhcBinding
Result: Negative

Epitope: M3
Chemical type: Peptide, no natural source
Sequence: GGGIRPFETKVK
Assay category: MhcBinding
Result: Negative

Epitope: M3
Chemical type: Peptide, no natural source
Sequence: GGGIRPFETKVK
Assay category: MhcBinding
Result: Negative

Epitope: W2
Chemical type: Peptide, no natural source
Sequence: GGYIRPLETKVK
Assay category: MhcBinding
Result: Negative

Epitope: W2
Chemical type: Peptide, no natural source
Sequence: GGYIRPLETKVK
Assay category: MhcBinding
Result: Negative

Epitope: W3
Chemical type: Peptide, no natural source
Sequence: GGGIRPLETKVK
Assay category: MhcBinding
Result: Negative

Epitope: W3
Chemical type: Peptide, no natural source
Sequence: GGGIRPLETKVK
Assay category: MhcBinding
Result: Negative

Epitope: Alms1 MUT
Chemical type: Peptide, no natural source
Sequence: LYLDSKSDTTV
Assay category: TCell
Result: Negative
Host: F 6-8 weeks

Epitope: Alms1 MUT
Chemical type: Peptide, no natural source
Sequence: LYLDSKSDTTV
Assay category: MhcLigandElution
Result: Positive

Epitope: Alms1 MUT
Chemical type: Peptide, no natural source
Sequence: LYLDSKSDTTV
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 62.25