Reference: Li-Xin Wang; Toshi Nagata; Kunio Tsujimura; Masato Uchijima; Shintaro Seto; Yukio Koide. "Identification of HLA-DR4-restricted T-cell epitope on MPT51 protein, a major secreted protein derived from Mycobacterium tuberculosis using MPT51 overlapping peptides screening and DNA vaccination." Vaccine (2010).

Epitope: p1 MPT51 1-20
Chemical type: Peptide from protein
Sequence: APYENIMVPSPSMGRDIPVA
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p1 MPT51 1-20
Chemical type: Peptide from protein
Sequence: APYENIMVPSPSMGRDIPVA
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p11 MPT51 11-30
Chemical type: Peptide from protein
Sequence: PSMGRDIPVAFLAGGPMAVY
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p11 MPT51 11-30
Chemical type: Peptide from protein
Sequence: PSMGRDIPVAFLAGGPMAVY
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p31 MPT51 31-50
Chemical type: Peptide from protein
Sequence: LLDAFNAGPDVSNWVTAGNA
Position: 64-83
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p31 MPT51 31-50
Chemical type: Peptide from protein
Sequence: LLDAFNAGPDVSNWVTAGNA
Position: 64-83
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p41 MPT51 41-60
Chemical type: Peptide from protein
Sequence: VSNWVTAGNAMNTLAGKGIS
Position: 85-104
Source molecule (GenBank): ZP_06507709.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p41 MPT51 41-60
Chemical type: Peptide from protein
Sequence: VSNWVTAGNAMNTLAGKGIS
Position: 85-104
Source molecule (GenBank): ZP_06507709.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p51 MPT51 51-70
Chemical type: Peptide from protein
Sequence: MNTLAGKGISVVAPAGGAYS
Position: 84-103
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p51 MPT51 51-70
Chemical type: Peptide from protein
Sequence: MNTLAGKGISVVAPAGGAYS
Position: 84-103
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p61 MPT51 61-80
Chemical type: Peptide from protein
Sequence: VVAPAGGAYSMYTNWEQDGS
Position: 94-113
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p61 MPT51 61-80
Chemical type: Peptide from protein
Sequence: VVAPAGGAYSMYTNWEQDGS
Position: 94-113
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p71 MPT51 71-90
Chemical type: Peptide from protein
Sequence: MYTNWEQDGSKQWDTFLSAE
Position: 104-123
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p71 MPT51 71-90
Chemical type: Peptide from protein
Sequence: MYTNWEQDGSKQWDTFLSAE
Position: 104-123
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p81 MPT51 81-100
Chemical type: Peptide from protein
Sequence: KQWDTFLSAELPDWLAANRG
Position: 114-133
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p81 MPT51 81-100
Chemical type: Peptide from protein
Sequence: KQWDTFLSAELPDWLAANRG
Position: 114-133
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p91 MPT51 91-110
Chemical type: Peptide from protein
Sequence: LPDWLAANRGLAPGGHAAVG
Position: 124-143
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p91 MPT51 91-110
Chemical type: Peptide from protein
Sequence: LPDWLAANRGLAPGGHAAVG
Position: 124-143
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p101 MPT51 101-120
Chemical type: Peptide from protein
Sequence: LAPGGHAAVGAAQGGYGAMA
Position: 134-153
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p101 MPT51 101-120
Chemical type: Peptide from protein
Sequence: LAPGGHAAVGAAQGGYGAMA
Position: 134-153
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p111 MPT51 111-130
Chemical type: Peptide from protein
Sequence: AAQGGYGAMALAAFHPDRFG
Position: 144-163
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p111 MPT51 111-130
Chemical type: Peptide from protein
Sequence: AAQGGYGAMALAAFHPDRFG
Position: 144-163
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p121 MPT51 121-140
Chemical type: Peptide from protein
Sequence: LAAFHPDRFGFAGSMSGFLY
Position: 154-173
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p121 MPT51 121-140
Chemical type: Peptide from protein
Sequence: LAAFHPDRFGFAGSMSGFLY
Position: 154-173
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p141 MPT51 141-160
Chemical type: Peptide from protein
Sequence: PSNTTTNGAIAAGMQQFGGV
Position: 174-193
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p141 MPT51 141-160
Chemical type: Peptide from protein
Sequence: PSNTTTNGAIAAGMQQFGGV
Position: 174-193
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p151 MPT51 151-170
Chemical type: Peptide from protein
Sequence: AAGMQQFGGVDTNGMWGAPQ
Position: 184-203
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p151 MPT51 151-170
Chemical type: Peptide from protein
Sequence: AAGMQQFGGVDTNGMWGAPQ
Position: 184-203
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p161 MPT51 161-180
Chemical type: Peptide from protein
Sequence: DTNGMWGAPQLGRWKWHDPW
Position: 194-213
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p161 MPT51 161-180
Chemical type: Peptide from protein
Sequence: DTNGMWGAPQLGRWKWHDPW
Position: 194-213
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p171 MPT51 171-190
Chemical type: Peptide from protein
Sequence: LGRWKWHDPWVHASLLAQNN
Position: 204-223
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p171 MPT51 171-190
Chemical type: Peptide from protein
Sequence: LGRWKWHDPWVHASLLAQNN
Position: 204-223
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p181 MPT51 181-200
Chemical type: Peptide from protein
Sequence: VHASLLAQNNTRVWVWSPTN
Position: 214-233
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p181 MPT51 181-200
Chemical type: Peptide from protein
Sequence: VHASLLAQNNTRVWVWSPTN
Position: 214-233
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p191 MPT51 191-211
Chemical type: Peptide from protein
Sequence: TRVWVWSPTNPGASDPAAMI
Position: 224-243
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Positive
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p191 MPT51 191-211
Chemical type: Peptide from protein
Sequence: TRVWVWSPTNPGASDPAAMI
Position: 224-243
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Positive
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p211 MPT51 211-230
Chemical type: Peptide from protein
Sequence: GQAAEAMGNSRMFYNQYRSV
Position: 244-263
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p211 MPT51 211-230
Chemical type: Peptide from protein
Sequence: GQAAEAMGNSRMFYNQYRSV
Position: 244-263
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p221 MPT51 221-240
Chemical type: Peptide from protein
Sequence: RMFYNQYRSVGGHNGHFDFP
Position: 254-273
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p221 MPT51 221-240
Chemical type: Peptide from protein
Sequence: RMFYNQYRSVGGHNGHFDFP
Position: 254-273
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p231 MPT51 231-250
Chemical type: Peptide from protein
Sequence: GGHNGHFDFPASGDNGWGSW
Position: 264-283
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p231 MPT51 231-250
Chemical type: Peptide from protein
Sequence: GGHNGHFDFPASGDNGWGSW
Position: 264-283
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p241 MPT51 241-260
Chemical type: Peptide from protein
Sequence: ASGDNGWGSWAPQLGAMSGD
Position: 274-293
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p241 MPT51 241-260
Chemical type: Peptide from protein
Sequence: ASGDNGWGSWAPQLGAMSGD
Position: 274-293
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Negative
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p191 MPT51 191-202
Chemical type: Peptide from protein
Sequence: TRVWVWSPTNP
Position: 224-234
Source molecule (GenBank): P0A4V6.1
Assay category: TCell
Result: Positive
Host: F 2-3 months
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo