Reference: M Widhe; C Ekerfelt; S Jarefors; B H Skogman; E M Peterson; S Bergström; P Forsberg; J Ernerudh. "T-cell epitope mapping of the Borrelia garinii outer surface protein A in lyme neuroborreliosis." Scandinavian journal of immunology (2009).

Epitope: OspA<sub>25-44</sub>
Chemical type: Peptide from protein
Sequence: DEKNSVSVDLPGGMQVLVSK
Position: 25-44
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  6-83 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>25-44</sub>
Chemical type: Peptide from protein
Sequence: DEKNSVSVDLPGGMQVLVSK
Position: 25-44
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  2-76 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>33-52</sub>
Chemical type: Peptide from protein
Sequence: DLPGGMQVLVSKEKDKDGKY
Position: 33-52
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  6-83 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>33-52</sub>
Chemical type: Peptide from protein
Sequence: DLPGGMQVLVSKEKDKDGKY
Position: 33-52
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  2-76 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>41-60</sub>
Chemical type: Peptide from protein
Sequence: LVSKEKDKDGKYSLMATVDK
Position: 41-60
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Negative
Host:  6-83 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>41-60</sub>
Chemical type: Peptide from protein
Sequence: LVSKEKDKDGKYSLMATVDK
Position: 41-60
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  2-76 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>49-68</sub>
Chemical type: Peptide from protein
Sequence: DGKYSLMATVDKLELKGTSD
Position: 49-68
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  6-83 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>49-68</sub>
Chemical type: Peptide from protein
Sequence: DGKYSLMATVDKLELKGTSD
Position: 49-68
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  2-76 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>57-76</sub>
Chemical type: Peptide from protein
Sequence: TVDKLELKGTSDKNNGSGTL
Position: 57-76
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  6-83 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>57-76</sub>
Chemical type: Peptide from protein
Sequence: TVDKLELKGTSDKNNGSGTL
Position: 57-76
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  2-76 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>65-84</sub>
Chemical type: Peptide from protein
Sequence: GTSDKNNGSGTLEGEKTDKS
Position: 65-84
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  6-83 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>65-84</sub>
Chemical type: Peptide from protein
Sequence: GTSDKNNGSGTLEGEKTDKS
Position: 65-84
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  2-76 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>73-92</sub>
Chemical type: Peptide from protein
Sequence: SGTLEGEKTDKSKAKLTIAE
Position: 73-92
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  2-76 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>73-92</sub>
Chemical type: Peptide from protein
Sequence: SGTLEGEKTDKSKAKLTIAE
Position: 73-92
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  6-83 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>81-100</sub>
Chemical type: Peptide from protein
Sequence: TDKSKAKLTIAEDLSKTTFE
Position: 81-100
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Negative
Host:  6-83 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>81-100</sub>
Chemical type: Peptide from protein
Sequence: TDKSKAKLTIAEDLSKTTFE
Position: 81-100
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  2-76 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>89-108</sub>
Chemical type: Peptide from protein
Sequence: TIAEDLSKTTFEIFKEDGKT
Position: 89-108
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  2-76 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>89-108</sub>
Chemical type: Peptide from protein
Sequence: TIAEDLSKTTFEIFKEDGKT
Position: 89-108
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  6-83 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>105-124</sub>
Chemical type: Peptide from protein
Sequence: DGKTLVSKKVTLKDKSSTEE
Position: 105-124
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  6-83 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>105-124</sub>
Chemical type: Peptide from protein
Sequence: DGKTLVSKKVTLKDKSSTEE
Position: 105-124
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  2-76 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>113-132</sub>
Chemical type: Peptide from protein
Sequence: KVTLKDKSSTEEKFNAKGEA
Position: 113-132
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  2-76 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>113-132</sub>
Chemical type: Peptide from protein
Sequence: KVTLKDKSSTEEKFNAKGEA
Position: 113-132
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  6-83 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>121-140</sub>
Chemical type: Peptide from protein
Sequence: SSTEEKFNAKGEASEKTIVRA
Position: 120-140
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  2-76 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>121-140</sub>
Chemical type: Peptide from protein
Sequence: SSTEEKFNAKGEASEKTIVRA
Position: 120-140
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  6-83 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>129-148</sub>
Chemical type: Peptide from protein
Sequence: KGEASEKTIVRANGTRLEYT
Position: 129-148
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  2-76 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>129-148</sub>
Chemical type: Peptide from protein
Sequence: KGEASEKTIVRANGTRLEYT
Position: 129-148
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Negative
Host:  6-83 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>137-156</sub>
Chemical type: Peptide from protein
Sequence: IVRANGTRLEYTDIKSDKTG
Position: 137-156
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  2-76 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>137-156</sub>
Chemical type: Peptide from protein
Sequence: IVRANGTRLEYTDIKSDKTG
Position: 137-156
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  6-83 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>145-164</sub>
Chemical type: Peptide from protein
Sequence: LEYTDIKSDKTGKAKEVLKD
Position: 145-164
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  6-83 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>145-164</sub>
Chemical type: Peptide from protein
Sequence: LEYTDIKSDKTGKAKEVLKD
Position: 145-164
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  2-76 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>153-172</sub>
Chemical type: Peptide from protein
Sequence: DKTGKAKEVLKDFALEGTLA
Position: 153-172
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  2-76 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>153-172</sub>
Chemical type: Peptide from protein
Sequence: DKTGKAKEVLKDFALEGTLA
Position: 153-172
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Negative
Host:  6-83 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>161-180</sub>
Chemical type: Peptide from protein
Sequence: VLKDFALEGTLAADGKTTLK
Position: 161-180
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Negative
Host:  6-83 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>161-180</sub>
Chemical type: Peptide from protein
Sequence: VLKDFALEGTLAADGKTTLK
Position: 161-180
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  2-76 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>193-212</sub>
Chemical type: Peptide from protein
Sequence: SNSGEITVELNDSDTTQATK
Position: 193-212
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  2-76 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>193-212</sub>
Chemical type: Peptide from protein
Sequence: SNSGEITVELNDSDTTQATK
Position: 193-212
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  6-83 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>201-220</sub>
Chemical type: Peptide from protein
Sequence: ELNDSDTTQATKKTGTWDSK
Position: 201-220
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  6-83 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>201-220</sub>
Chemical type: Peptide from protein
Sequence: ELNDSDTTQATKKTGTWDSK
Position: 201-220
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  2-76 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>217-236</sub>
Chemical type: Peptide from protein
Sequence: WDSKTSTLTISVNSRKTKNL
Position: 217-236
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  2-76 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>217-236</sub>
Chemical type: Peptide from protein
Sequence: WDSKTSTLTISVNSRKTKNL
Position: 217-236
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Negative
Host:  6-83 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>233-252</sub>
Chemical type: Peptide from protein
Sequence: TKNLVFTKEDTITVQKYDSA
Position: 233-252
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  2-76 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>233-252</sub>
Chemical type: Peptide from protein
Sequence: TKNLVFTKEDTITVQKYDSA
Position: 233-252
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Negative
Host:  6-83 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>241-260</sub>
Chemical type: Peptide from protein
Sequence: EDTITVQKYDSAGTNLEGKA
Position: 241-260
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  2-76 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>241-260</sub>
Chemical type: Peptide from protein
Sequence: EDTITVQKYDSAGTNLEGKA
Position: 241-260
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Negative
Host:  6-83 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>249-268</sub>
Chemical type: Peptide from protein
Sequence: YDSAGTNLEGKAVEITTLKE
Position: 249-268
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  2-76 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>249-268</sub>
Chemical type: Peptide from protein
Sequence: YDSAGTNLEGKAVEITTLKE
Position: 249-268
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  6-83 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>257-274</sub>
Chemical type: Peptide from protein
Sequence: EGKAVEITTLKELKDALK
Position: 257-274
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  6-83 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: OspA<sub>257-274</sub>
Chemical type: Peptide from protein
Sequence: EGKAVEITTLKELKDALK
Position: 257-274
Source molecule (GenBank): AAO91921.1
Assay category: TCell
Result: Positive
Host:  2-76 years
Cell type: mononuclear cell
Tissue: cerebrospinal fluid (CSF)
Culture conditions: Direct Ex Vivo