Reference: Jason Botten; Jeff Alexander; Valerie Pasquetto; John Sidney; Polly Barrowman; Joey Ting; Bjoern Peters; Scott Southwood; Barbara Stewart; Maria P Rodriguez-Carreno; Bianca Mothe; J Lindsay Whitton; Alessandro Sette; Michael J Buchmeier. "Identification of protective Lassa virus epitopes that are restricted by HLA-A2." Journal of virology (2006).

Epitope: NP 530-539
Chemical type: Peptide from protein
Sequence: ALMDCIMFDA
Position: 530-539
Source molecule (GenBank): P13699.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 530-539
Chemical type: Peptide from protein
Sequence: ALMDCIMFDA
Position: 530-539
Source molecule (GenBank): P13699.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: NP 530-539
Chemical type: Peptide from protein
Sequence: ALMDCIMFDA
Position: 530-539
Source molecule (GenBank): P13699.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 2.0

Epitope: GPC 434-442
Chemical type: Peptide from protein
Sequence: FVFSTSFYL
Position: 434-442
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GPC 434-442
Chemical type: Peptide from protein
Sequence: FVFSTSFYL
Position: 434-442
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GPC 434-442
Chemical type: Peptide from protein
Sequence: FVFSTSFYL
Position: 434-442
Source molecule (GenBank): P08669.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 5.0

Epitope: GPC 127-136
Chemical type: Peptide from protein
Sequence: NLYDHALMSI
Position: 127-136
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GPC 127-136
Chemical type: Peptide from protein
Sequence: NLYDHALMSI
Position: 127-136
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GPC 127-136
Chemical type: Peptide from protein
Sequence: NLYDHALMSI
Position: 127-136
Source molecule (GenBank): P08669.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 8.0

Epitope: GPC 257-266
Chemical type: Peptide from protein
Sequence: RLLGTFTWTL
Position: 257-266
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GPC 257-266
Chemical type: Peptide from protein
Sequence: RLLGTFTWTL
Position: 257-266
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GPC 257-266
Chemical type: Peptide from protein
Sequence: RLLGTFTWTL
Position: 257-266
Source molecule (GenBank): P08669.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 10.0

Epitope: GPC 441-450
Chemical type: Peptide from protein
Sequence: YLISIFLHLV
Position: 441-450
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GPC 441-450
Chemical type: Peptide from protein
Sequence: YLISIFLHLV
Position: 441-450
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GPC 441-450
Chemical type: Peptide from protein
Sequence: YLISIFLHLV
Position: 441-450
Source molecule (GenBank): P08669.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 12.0

Epitope: GPC 441-449
Chemical type: Peptide from protein
Sequence: YLISIFLHL
Position: 441-449
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GPC 441-449
Chemical type: Peptide from protein
Sequence: YLISIFLHL
Position: 441-449
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GPC 441-449
Chemical type: Peptide from protein
Sequence: YLISIFLHL
Position: 441-449
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GPC 441-449
Chemical type: Peptide from protein
Sequence: YLISIFLHL
Position: 441-449
Source molecule (GenBank): P08669.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 11.0

Epitope: GPC 441-449
Chemical type: Peptide from protein
Sequence: YLISIFLHL
Position: 441-449
Source molecule (GenBank): P08669.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 10.0

Epitope: GPC 441-449
Chemical type: Peptide from protein
Sequence: YLISIFLHL
Position: 441-449
Source molecule (GenBank): P08669.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 14.0

Epitope: GPC 441-449
Chemical type: Peptide from protein
Sequence: YLISIFLHL
Position: 441-449
Source molecule (GenBank): P08669.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 6657.0

Epitope: GPC 441-449
Chemical type: Peptide from protein
Sequence: YLISIFLHL
Position: 441-449
Source molecule (GenBank): P08669.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 173.0

Epitope: GPC 34-43
Chemical type: Peptide from protein
Sequence: GLYNFATCGL
Position: 34-43
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GPC 34-43
Chemical type: Peptide from protein
Sequence: GLYNFATCGL
Position: 34-43
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GPC 34-43
Chemical type: Peptide from protein
Sequence: GLYNFATCGL
Position: 34-43
Source molecule (GenBank): P08669.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 31.0

Epitope: GPC 60-68
Chemical type: Peptide from protein
Sequence: SLYKGVYEL
Position: 60-68
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GPC 60-68
Chemical type: Peptide from protein
Sequence: SLYKGVYEL
Position: 60-68
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GPC 60-68
Chemical type: Peptide from protein
Sequence: SLYKGVYEL
Position: 60-68
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GPC 60-68
Chemical type: Peptide from protein
Sequence: SLYKGVYEL
Position: 60-68
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks

Epitope: GPC 60-68
Chemical type: Peptide from protein
Sequence: SLYKGVYEL
Position: 60-68
Source molecule (GenBank): P08669.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 115.0

Epitope: GPC 60-68
Chemical type: Peptide from protein
Sequence: SLYKGVYEL
Position: 60-68
Source molecule (GenBank): P08669.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 10000.0

Epitope: GPC 60-68
Chemical type: Peptide from protein
Sequence: SLYKGVYEL
Position: 60-68
Source molecule (GenBank): P08669.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 12.0

Epitope: GPC 60-68
Chemical type: Peptide from protein
Sequence: SLYKGVYEL
Position: 60-68
Source molecule (GenBank): P08669.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 47.0

Epitope: GPC 60-68
Chemical type: Peptide from protein
Sequence: SLYKGVYEL
Position: 60-68
Source molecule (GenBank): P08669.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 395.0

Epitope: GPC 213-221
Chemical type: Peptide from protein
Sequence: IMTSYQYLI
Position: 213-221
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GPC 213-221
Chemical type: Peptide from protein
Sequence: IMTSYQYLI
Position: 213-221
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GPC 213-221
Chemical type: Peptide from protein
Sequence: IMTSYQYLI
Position: 213-221
Source molecule (GenBank): P08669.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 67.0

Epitope: GPC 257-265
Chemical type: Peptide from protein
Sequence: RLLGTFTWT
Position: 257-265
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GPC 257-265
Chemical type: Peptide from protein
Sequence: RLLGTFTWT
Position: 257-265
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GPC 257-265
Chemical type: Peptide from protein
Sequence: RLLGTFTWT
Position: 257-265
Source molecule (GenBank): P08669.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 67.0

Epitope: GPC 478-487
Chemical type: Peptide from protein
Sequence: GLYKQPGVPV
Position: 478-487
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GPC 478-487
Chemical type: Peptide from protein
Sequence: GLYKQPGVPV
Position: 478-487
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GPC 478-487
Chemical type: Peptide from protein
Sequence: GLYKQPGVPV
Position: 478-487
Source molecule (GenBank): P08669.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 89.0

Epitope: NP 101-109
Chemical type: Peptide from protein
Sequence: ILAADLEKL
Position: 101-109
Source molecule (GenBank): P13699.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 101-109
Chemical type: Peptide from protein
Sequence: ILAADLEKL
Position: 101-109
Source molecule (GenBank): P13699.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 92.0

Epitope: GPC 442-450
Chemical type: Peptide from protein
Sequence: LISIFLHLV
Position: 442-450
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GPC 442-450
Chemical type: Peptide from protein
Sequence: LISIFLHLV
Position: 442-450
Source molecule (GenBank): P08669.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 95.0

Epitope: GPC 23-31
Chemical type: Peptide from protein
Sequence: LIALSVLAV
Position: 23-31
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GPC 23-31
Chemical type: Peptide from protein
Sequence: LIALSVLAV
Position: 23-31
Source molecule (GenBank): P08669.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 102.0

Epitope: GPC 133-142
Chemical type: Peptide from protein
Sequence: LMSIISTFHL
Position: 133-142
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GPC 133-142
Chemical type: Peptide from protein
Sequence: LMSIISTFHL
Position: 133-142
Source molecule (GenBank): P08669.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 113.0

Epitope: NP 38-47
Chemical type: Peptide from protein
Sequence: LLHGLDFSEV
Position: 38-47
Source molecule (GenBank): P13699.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 38-47
Chemical type: Peptide from protein
Sequence: LLHGLDFSEV
Position: 38-47
Source molecule (GenBank): P13699.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 132.0

Epitope: NP 240-248
Chemical type: Peptide from protein
Sequence: NISGYNFSL
Position: 240-248
Source molecule (GenBank): P13699.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: NP 240-248
Chemical type: Peptide from protein
Sequence: NISGYNFSL
Position: 240-248
Source molecule (GenBank): P13699.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 240-248
Chemical type: Peptide from protein
Sequence: NISGYNFSL
Position: 240-248
Source molecule (GenBank): P13699.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 138.0

Epitope: NP 444-452
Chemical type: Peptide from protein
Sequence: GLTSAVIDA
Position: 444-452
Source molecule (GenBank): P13699.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 444-452
Chemical type: Peptide from protein
Sequence: GLTSAVIDA
Position: 444-452
Source molecule (GenBank): P13699.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 198.0

Epitope: NP 66-74
Chemical type: Peptide from protein
Sequence: RLRDLNQAV
Position: 66-74
Source molecule (GenBank): P13699.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 66-74
Chemical type: Peptide from protein
Sequence: RLRDLNQAV
Position: 66-74
Source molecule (GenBank): P13699.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 259.0

Epitope: GPC 136-144
Chemical type: Peptide from protein
Sequence: IISTFHLSI
Position: 136-144
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GPC 136-144
Chemical type: Peptide from protein
Sequence: IISTFHLSI
Position: 136-144
Source molecule (GenBank): P08669.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 330.0

Epitope: NP 376-384
Chemical type: Peptide from protein
Sequence: AMLQLDPNA
Position: 376-384
Source molecule (GenBank): P13699.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 376-384
Chemical type: Peptide from protein
Sequence: AMLQLDPNA
Position: 376-384
Source molecule (GenBank): P13699.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 383.0

Epitope: NP 365-373
Chemical type: Peptide from protein
Sequence: LTYSQLMTL
Position: 365-373
Source molecule (GenBank): P13699.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 365-373
Chemical type: Peptide from protein
Sequence: LTYSQLMTL
Position: 365-373
Source molecule (GenBank): P13699.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: NP 365-373
Chemical type: Peptide from protein
Sequence: LTYSQLMTL
Position: 365-373
Source molecule (GenBank): P13699.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 454.0

Epitope: GPC 22-31
Chemical type: Peptide from protein
Sequence: VLIALSVLAV
Position: 22-31
Source molecule (GenBank): P08669.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GPC 22-31
Chemical type: Peptide from protein
Sequence: VLIALSVLAV
Position: 22-31
Source molecule (GenBank): P08669.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 485.0

Epitope: GPC 59-67
Chemical type: Peptide from protein
Sequence: LIYKGTYEL
Position: 59-67
Source molecule (GenBank): P17332.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GPC 440-448
Chemical type: Peptide from protein
Sequence: YLISIFLHL
Position: 440-448
Source molecule (GenBank): P17332.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GPC 440-448
Chemical type: Peptide from protein
Sequence: YLISIFLHL
Position: 440-448
Source molecule (GenBank): P17332.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GPC 440-448
Chemical type: Peptide from protein
Sequence: YLISIFLHL
Position: 440-448
Source molecule (GenBank): P17332.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GPC 440-448
Chemical type: Peptide from protein
Sequence: YLISIFLHL
Position: 440-448
Source molecule (GenBank): P17332.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 14.0

Epitope: GPC 440-448
Chemical type: Peptide from protein
Sequence: YLISIFLHL
Position: 440-448
Source molecule (GenBank): P17332.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 10.0

Epitope: GPC 440-448
Chemical type: Peptide from protein
Sequence: YLISIFLHL
Position: 440-448
Source molecule (GenBank): P17332.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 173.0

Epitope: GPC 440-448
Chemical type: Peptide from protein
Sequence: YLISIFLHL
Position: 440-448
Source molecule (GenBank): P17332.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 6657.0

Epitope: GPC 440-448
Chemical type: Peptide from protein
Sequence: YLISIFLHL
Position: 440-448
Source molecule (GenBank): P17332.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 11.0