Reference: Aklile Berhanu; Rebecca L Wilson; Dana L Kirkwood-Watts; David S King; Travis K Warren; Susan A Lund; Lindsay L Brown; Alex K Krupkin; Erin Vandermay; Will Weimers; Kady M Honeychurch; Douglas W Grosenbach; Kevin F Jones; Dennis E Hruby. "Vaccination of BALB/c mice with Escherichia coli-expressed vaccinia virus proteins A27L, B5R, and D8L protects mice from lethal vaccinia virus challenge." Journal of virology (2008).

Epitope: B5R-14/15
Chemical type: Peptide from protein
Sequence: EYMTINCDVG
Position: 151-160
Source molecule (GenBank): P21115.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B5R-14/15
Chemical type: Peptide from protein
Sequence: EYMTINCDVG
Position: 151-160
Source molecule (GenBank): P21115.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D8L-12/13
Chemical type: Peptide from protein
Sequence: FQKIVNQLDS
Position: 131-140
Source molecule (GenBank): P20508.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D8L-12/13
Chemical type: Peptide from protein
Sequence: FQKIVNQLDS
Position: 131-140
Source molecule (GenBank): P20508.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A27L-1
Chemical type: Peptide from protein
Sequence: MDGTLFPGDDDLAIPATEFF
Position: 1-20
Source molecule (GenBank): P20535.1
Assay category: BCell
Result: Positive-Intermediate
Host: F 6 weeks

Epitope: A27L-1
Chemical type: Peptide from protein
Sequence: MDGTLFPGDDDLAIPATEFF
Position: 1-20
Source molecule (GenBank): P20535.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A27L-1
Chemical type: Peptide from protein
Sequence: MDGTLFPGDDDLAIPATEFF
Position: 1-20
Source molecule (GenBank): P20535.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A27L-4
Chemical type: Peptide from protein
Sequence: KREAIVKADEDDNEETLKQR
Position: 31-50
Source molecule (GenBank): P20535.1
Assay category: BCell
Result: Positive-Intermediate
Host: F 6 weeks

Epitope: A27L-5
Chemical type: Peptide from protein
Sequence: DDNEETLKQRLTNLEKKITN
Position: 41-60
Source molecule (GenBank): P20535.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: A27L-6
Chemical type: Peptide from protein
Sequence: LTNLEKKITNVTTKFEQIEK
Position: 51-70
Source molecule (GenBank): P20535.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: A27L-7
Chemical type: Peptide from protein
Sequence: VTTKFEQIEKCCKRNDEVLF
Position: 61-80
Source molecule (GenBank): P20535.1
Assay category: BCell
Result: Positive-Low
Host: F 6 weeks

Epitope: A27L-10
Chemical type: Peptide from protein
Sequence: AAMISLAKKIDVQTGRRPYE
Position: 91-110
Source molecule (GenBank): P20535.1
Assay category: BCell
Result: Positive-Intermediate
Host: F 6 weeks

Epitope: A27L-10
Chemical type: Peptide from protein
Sequence: AAMISLAKKIDVQTGRRPYE
Position: 91-110
Source molecule (GenBank): P20535.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A27L-10
Chemical type: Peptide from protein
Sequence: AAMISLAKKIDVQTGRRPYE
Position: 91-110
Source molecule (GenBank): P20535.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B5R-1
Chemical type: Peptide from protein
Sequence: MKTISVVTLLCVLPAVVYST
Position: 1-20
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: B5R-2
Chemical type: Peptide from protein
Sequence: CVLPAVVYSTCTVPTMNNAK
Position: 11-30
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: B5R-3
Chemical type: Peptide from protein
Sequence: CTVPTMNNAKLTSTETSFNN
Position: 21-40
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: B5R-4
Chemical type: Peptide from protein
Sequence: LTSTETSFNNNQKVTFTCDQ
Position: 31-50
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: B5R-5
Chemical type: Peptide from protein
Sequence: NQKVTFTCDQGYHSSDPNAV
Position: 41-60
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: B5R-6
Chemical type: Peptide from protein
Sequence: GYHSSDPNAVCETDKWKYEN
Position: 51-70
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Positive-Low
Host: F 6 weeks

Epitope: B5R-7
Chemical type: Peptide from protein
Sequence: CETDKWKYENPCKKMCTVSD
Position: 61-80
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Positive-Low
Host: F 6 weeks

Epitope: B5R-8
Chemical type: Peptide from protein
Sequence: PCKKMCTVSDYISELYNKPL
Position: 71-90
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Positive-Low
Host: F 6 weeks

Epitope: B5R-9
Chemical type: Peptide from protein
Sequence: YISELYNKPLYEVNSTMTLS
Position: 81-100
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: B5R-9
Chemical type: Peptide from protein
Sequence: YISELYNKPLYEVNSTMTLS
Position: 81-100
Source molecule (GenBank): P21115.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B5R-9
Chemical type: Peptide from protein
Sequence: YISELYNKPLYEVNSTMTLS
Position: 81-100
Source molecule (GenBank): P21115.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B5R-10
Chemical type: Peptide from protein
Sequence: CNGETKYFRCEEKNGNTSWN
Position: 101-120
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Positive-Low
Host: F 6 weeks

Epitope: B5R-11
Chemical type: Peptide from protein
Sequence: EEKNGNTSWNDTVTCPNAEC
Position: 111-130
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Positive-Low
Host: F 6 weeks

Epitope: B5R-12
Chemical type: Peptide from protein
Sequence: DTVTCPNAECQPLQLEHGSC
Position: 121-140
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Positive-Low
Host: F 6 weeks

Epitope: B5R-13
Chemical type: Peptide from protein
Sequence: QPLQLEHGSCQPVKEKYSFG
Position: 131-150
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: B5R-14
Chemical type: Peptide from protein
Sequence: QPVKEKYSFGEYMTINCDVG
Position: 141-160
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Positive-Low
Host: F 6 weeks

Epitope: B5R-14
Chemical type: Peptide from protein
Sequence: QPVKEKYSFGEYMTINCDVG
Position: 141-160
Source molecule (GenBank): P21115.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B5R-14
Chemical type: Peptide from protein
Sequence: QPVKEKYSFGEYMTINCDVG
Position: 141-160
Source molecule (GenBank): P21115.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B5R-15
Chemical type: Peptide from protein
Sequence: EYMTINCDVGYEVIGASYIS
Position: 151-170
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: B5R-15
Chemical type: Peptide from protein
Sequence: EYMTINCDVGYEVIGASYIS
Position: 151-170
Source molecule (GenBank): P21115.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B5R-15
Chemical type: Peptide from protein
Sequence: EYMTINCDVGYEVIGASYIS
Position: 151-170
Source molecule (GenBank): P21115.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B5R-16
Chemical type: Peptide from protein
Sequence: YEVIGASYISCTANSWNVIP
Position: 161-180
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: B5R-17
Chemical type: Peptide from protein
Sequence: CTANSWNVIPSCQQKCDIPS
Position: 171-190
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Positive-Intermediate
Host: F 6 weeks

Epitope: B5R-18
Chemical type: Peptide from protein
Sequence: SCQQKCDIPSLSNGLISGST
Position: 181-200
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Positive-Low
Host: F 6 weeks

Epitope: B5R-19
Chemical type: Peptide from protein
Sequence: LSNGLISGSTFSIGGVIHLS
Position: 191-210
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: B5R-20
Chemical type: Peptide from protein
Sequence: FSIGGVIHLSCKSGFILTGS
Position: 201-220
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: B5R-22
Chemical type: Peptide from protein
Sequence: PSSTCIDGKWNPVLPICVRT
Position: 221-240
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Positive-Low
Host: F 6 weeks

Epitope: B5R-23
Chemical type: Peptide from protein
Sequence: NPVLPICVRTNEEFDPVDDG
Position: 231-250
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Positive-High
Host: F 6 weeks

Epitope: B5R-24
Chemical type: Peptide from protein
Sequence: NEEFDPVDDGPDDETDLSKL
Position: 241-260
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Positive-High
Host: F 6 weeks

Epitope: B5R-25
Chemical type: Peptide from protein
Sequence: PDDETDLSKLSKDVVQYEQE
Position: 251-270
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Positive-High
Host: F 6 weeks

Epitope: B5R-26
Chemical type: Peptide from protein
Sequence: SKDVVQYEQEIESLEATYHI
Position: 261-280
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Positive-High
Host: F 6 weeks

Epitope: B5R-26
Chemical type: Peptide from protein
Sequence: SKDVVQYEQEIESLEATYHI
Position: 261-280
Source molecule (GenBank): P21115.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B5R-26
Chemical type: Peptide from protein
Sequence: SKDVVQYEQEIESLEATYHI
Position: 261-280
Source molecule (GenBank): P21115.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B5R-27
Chemical type: Peptide from protein
Sequence: IESLEATYHIIIVALTIMGV
Position: 271-290
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Positive-Low
Host: F 6 weeks

Epitope: B5R-28
Chemical type: Peptide from protein
Sequence: IIVALTIMGVIFLISVIVLV
Position: 281-300
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: B5R-29
Chemical type: Peptide from protein
Sequence: CSCDKNNDQYKFHKLLP
Position: 301-317
Source molecule (GenBank): P21115.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: D8L-1
Chemical type: Peptide from protein
Sequence: MPQQLSPINIETKKAISNAR
Position: 1-20
Source molecule (GenBank): P20508.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: D8L-3
Chemical type: Peptide from protein
Sequence: LKPLDIHYNESKPTTIQNTG
Position: 21-40
Source molecule (GenBank): P20508.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: D8L-4
Chemical type: Peptide from protein
Sequence: SKPTTIQNTGKLVRINFKGG
Position: 31-50
Source molecule (GenBank): P20508.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: D8L-5
Chemical type: Peptide from protein
Sequence: KLVRINFKGGYISGGFLPNE
Position: 41-60
Source molecule (GenBank): P20508.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: D8L-6
Chemical type: Peptide from protein
Sequence: YISGGFLPNEYVLSSLHIYW
Position: 51-70
Source molecule (GenBank): P20508.1
Assay category: BCell
Result: Positive-Low
Host: F 6 weeks

Epitope: D8L-7
Chemical type: Peptide from protein
Sequence: YVLSSLHIYWGKEDDYGSNH
Position: 61-80
Source molecule (GenBank): P20508.1
Assay category: BCell
Result: Positive-Low
Host: F 6 weeks

Epitope: D8L-7
Chemical type: Peptide from protein
Sequence: YVLSSLHIYWGKEDDYGSNH
Position: 61-80
Source molecule (GenBank): P20508.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D8L-7
Chemical type: Peptide from protein
Sequence: YVLSSLHIYWGKEDDYGSNH
Position: 61-80
Source molecule (GenBank): P20508.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D8L-8
Chemical type: Peptide from protein
Sequence: GKEDDYGSNHLIDVYKYSGE
Position: 71-90
Source molecule (GenBank): P20508.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: D8L-10
Chemical type: Peptide from protein
Sequence: INLVHWNKKKYSSYEEAKKH
Position: 91-110
Source molecule (GenBank): P20508.1
Assay category: BCell
Result: Positive-Intermediate
Host: F 6 weeks

Epitope: D8L-11
Chemical type: Peptide from protein
Sequence: YSSYEEAKKHDDGLIIISIF
Position: 101-120
Source molecule (GenBank): P20508.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: D8L-12
Chemical type: Peptide from protein
Sequence: LQVSDHKNVYFQKIVNQLDS
Position: 121-140
Source molecule (GenBank): P20508.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: D8L-12
Chemical type: Peptide from protein
Sequence: LQVSDHKNVYFQKIVNQLDS
Position: 121-140
Source molecule (GenBank): P20508.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D8L-12
Chemical type: Peptide from protein
Sequence: LQVSDHKNVYFQKIVNQLDS
Position: 121-140
Source molecule (GenBank): P20508.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D8L-13
Chemical type: Peptide from protein
Sequence: FQKIVNQLDSIRSANTSAPF
Position: 131-150
Source molecule (GenBank): P20508.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: D8L-13
Chemical type: Peptide from protein
Sequence: FQKIVNQLDSIRSANTSAPF
Position: 131-150
Source molecule (GenBank): P20508.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D8L-13
Chemical type: Peptide from protein
Sequence: FQKIVNQLDSIRSANTSAPF
Position: 131-150
Source molecule (GenBank): P20508.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D8L-14
Chemical type: Peptide from protein
Sequence: IRSANTSAPFDSVFYLDNLL
Position: 141-160
Source molecule (GenBank): P20508.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: D8L-15
Chemical type: Peptide from protein
Sequence: DSVFYLDNLLPSTLDYFTYL
Position: 151-170
Source molecule (GenBank): P20508.1
Assay category: BCell
Result: Positive-Low
Host: F 6 weeks

Epitope: D8L-16
Chemical type: Peptide from protein
Sequence: PSTLDYFTYLGTTIKHSADA
Position: 161-180
Source molecule (GenBank): P20508.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: D8L-17
Chemical type: Peptide from protein
Sequence: GTTIKHSADAVWIIFPTPIN
Position: 171-190
Source molecule (GenBank): P20508.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: D8L-20
Chemical type: Peptide from protein
Sequence: KPYYITENYRNPYKLNDDTQ
Position: 211-230
Source molecule (GenBank): P20508.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: D8L-20
Chemical type: Peptide from protein
Sequence: KPYYITENYRNPYKLNDDTQ
Position: 211-230
Source molecule (GenBank): P20508.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D8L-20
Chemical type: Peptide from protein
Sequence: KPYYITENYRNPYKLNDDTQ
Position: 211-230
Source molecule (GenBank): P20508.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D8L-21
Chemical type: Peptide from protein
Sequence: NPYKLNDDTQVYYSGEIIRA
Position: 221-240
Source molecule (GenBank): P20508.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: D8L-22
Chemical type: Peptide from protein
Sequence: VYYSGEIIRAATTSPARENY
Position: 231-250
Source molecule (GenBank): P20508.1
Assay category: BCell
Result: Positive-Low
Host: F 6 weeks

Epitope: D8L-24
Chemical type: Peptide from protein
Sequence: FMRWLSDLRETCFSYYQKYI
Position: 251-270
Source molecule (GenBank): P20508.1
Assay category: BCell
Result: Positive-Low
Host: F 6 weeks

Epitope: D8L-25
Chemical type: Peptide from protein
Sequence: TCFSYYQKYIEGNKTFAIIA
Position: 261-280
Source molecule (GenBank): P20508.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: D8L-26
Chemical type: Peptide from protein
Sequence: EGNKTFAIIAIVFVFILTAI
Position: 271-290
Source molecule (GenBank): P20508.1
Assay category: BCell
Result: Negative
Host: F 6 weeks

Epitope: D8L-28
Chemical type: Peptide from protein
Sequence: LFLMSRRYSREKQN
Position: 291-304
Source molecule (GenBank): P20508.1
Assay category: BCell
Result: Negative
Host: F 6 weeks