Reference: N Thema; S I Tshilwane; A Pretorius; L Son; R M Smith; H C Steyn; J Liebenberg; M van Kleef. "Identification and characterisation of conserved epitopes of E. ruminantium that activate Th1 CD4<sup>+</sup> T cells: Towards the development of a multi-epitope vaccine." Molecular immunology (2019).

Epitope: p0660-42
Chemical type: Peptide from protein
Sequence: DIRAILSVDGLFDSKA
Position: 329-344
Source molecule (GenBank): WP_011154750.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p0660-42
Chemical type: Peptide from protein
Sequence: DIRAILSVDGLFDSKA
Position: 329-344
Source molecule (GenBank): WP_011154750.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p1150-18
Chemical type: Peptide from protein
Sequence: NGINDEDLGGMYGLLL
Position: 137-152
Source molecule (GenBank): WP_011154799.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p1150-19
Chemical type: Peptide from protein
Sequence: GGMYGLLLLGGFFSVM
Position: 145-160
Source molecule (GenBank): WP_011154799.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p2540-p6
Chemical type: Peptide from protein
Sequence: KNRTINIGVEFRIQDG
Position: 41-56
Source molecule (GenBank): WP_011255419.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p2540-p16
Chemical type: Peptide from protein
Sequence: HDNLNTKELSISLRIK
Position: 121-136
Source molecule (GenBank): WP_011154938.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p2540-p19
Chemical type: Peptide from protein
Sequence: IPQEKVIILNRFLQDY
Position: 145-160
Source molecule (GenBank): WP_011154938.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p2540-p20
Chemical type: Peptide from protein
Sequence: LNRFLQDYVNQENLGL
Position: 153-168
Source molecule (GenBank): WP_011154938.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p2540-p21
Chemical type: Peptide from protein
Sequence: VNQENLGLINFWKKK
Position: 161-175
Source molecule (GenBank): WP_011154938.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p5420-13
Chemical type: Peptide from protein
Sequence: TSNNIFHSNVYKDIVM
Position: 97-112
Source molecule (GenBank): WP_011154938.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p5420-13
Chemical type: Peptide from protein
Sequence: TSNNIFHSNVYKDIVM
Position: 97-112
Source molecule (GenBank): WP_011154938.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p5420-14
Chemical type: Peptide from protein
Sequence: NVYKDIVMFPISFALK
Position: 105-120
Source molecule (GenBank): WP_011154938.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p5420-14
Chemical type: Peptide from protein
Sequence: NVYKDIVMFPISFALK
Position: 105-120
Source molecule (GenBank): WP_011154938.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p7140-p6
Chemical type: Peptide from protein
Sequence: ELDKVELPKTRARETS
Position: 44-59
Source molecule (GenBank): WP_011256157.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p7140-p6
Chemical type: Peptide from protein
Sequence: ELDKVELPKTRARETS
Position: 44-59
Source molecule (GenBank): WP_011256157.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p7140-p7
Chemical type: Peptide from protein
Sequence: KTRARETSSDITVISD
Position: 52-67
Source molecule (GenBank): WP_011256157.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p7140-p7
Chemical type: Peptide from protein
Sequence: KTRARETSSDITVISD
Position: 52-67
Source molecule (GenBank): WP_011256157.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p7140-p12
Chemical type: Peptide from protein
Sequence: QQCCECSSSFGVKWSH
Position: 92-107
Source molecule (GenBank): WP_011256157.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p7140-p12
Chemical type: Peptide from protein
Sequence: QQCCECSSSFGVKWSH
Position: 92-107
Source molecule (GenBank): WP_011256157.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p7140-p13
Chemical type: Peptide from protein
Sequence: SFGVKWSHNDTSDKSD
Position: 100-115
Source molecule (GenBank): WP_011256157.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p7140-p13
Chemical type: Peptide from protein
Sequence: SFGVKWSHNDTSDKSD
Position: 100-115
Source molecule (GenBank): WP_011256157.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p7140-p20
Chemical type: Peptide from protein
Sequence: KAEDKVVKAAQIQDVP
Position: 156-171
Source molecule (GenBank): WP_011256157.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p7140-p20
Chemical type: Peptide from protein
Sequence: KAEDKVVKAAQIQDVP
Position: 156-171
Source molecule (GenBank): WP_011256157.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p7320-p8
Chemical type: Peptide from protein
Sequence: VYNITRVSSTSSSSLS
Position: 56-71
Source molecule (GenBank): WP_011255835.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p7320-p8
Chemical type: Peptide from protein
Sequence: VYNITRVSSTSSSSLS
Position: 56-71
Source molecule (GenBank): WP_011255835.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p7320-p9
Chemical type: Peptide from protein
Sequence: STSSSSLSTLSPTTII
Position: 64-79
Source molecule (GenBank): WP_011255835.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p7320-p9
Chemical type: Peptide from protein
Sequence: STSSSSLSTLSPTTII
Position: 64-79
Source molecule (GenBank): WP_011255835.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p7320-p21
Chemical type: Peptide from protein
Sequence: IVSSDTSNNGSVAEEN
Position: 164-179
Source molecule (GenBank): WP_011255835.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p7320-p21
Chemical type: Peptide from protein
Sequence: IVSSDTSNNGSVAEEN
Position: 164-179
Source molecule (GenBank): WP_011255835.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p7350-9
Chemical type: Peptide from protein
Sequence: MVSICCQGTSLGGFSE
Position: 1-16
Source molecule (GenBank): WP_011155413.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p7360-8
Chemical type: Peptide from protein
Sequence: VVIVMDLCCQIAGLLC
Position: 57-72
Source molecule (GenBank): WP_011155414.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p7360-8
Chemical type: Peptide from protein
Sequence: VVIVMDLCCQIAGLLC
Position: 57-72
Source molecule (GenBank): WP_011155414.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p7620-p2
Chemical type: Peptide from protein
Sequence: HPKKDSLIFVQDGFSL
Position: 9-24
Source molecule (GenBank): WP_011255858.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p7620-p2
Chemical type: Peptide from protein
Sequence: HPKKDSLIFVQDGFSL
Position: 9-24
Source molecule (GenBank): WP_011255858.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p7620-p12
Chemical type: Peptide from protein
Sequence: TKLKRMGYKIYNVIFA
Position: 89-104
Source molecule (GenBank): WP_011155440.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p7620-p12
Chemical type: Peptide from protein
Sequence: TKLKRMGYKIYNVIFA
Position: 89-104
Source molecule (GenBank): WP_011155440.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: p8010-p8
Chemical type: Peptide from protein
Sequence: LGSSIMAIFGKLPWPA
Position: 70-85
Source molecule (GenBank): WP_011255892.1
Assay category: TCell
Result: Positive
Host:  8-12 months
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo