Reference: Dongmin Zhao; Kaikai Han; Lijiao Zhang; Huili Wang; Yujie Tian; Xinmei Huang; Qingtao Liu; Jing Yang; Yuzhuo Liu; Yin Li. "Identification and immunogenic evaluation of T cell epitopes based on tembusu virus envelope protein in ducks." Virus research (2018).

Epitope: 1
Chemical type: Peptide from protein
Sequence: SLFGGMSWI
Position: 452-460
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Negative
Host:  25 days
Cell type: lymphocyte
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 1
Chemical type: Peptide from protein
Sequence: SLFGGMSWI
Position: 452-460
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Negative
Host: F 3 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 2
Chemical type: Peptide from protein
Sequence: RLITVNPYV
Position: 354-362
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Negative
Host: F 3 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 2
Chemical type: Peptide from protein
Sequence: RLITVNPYV
Position: 354-362
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Negative
Host:  25 days
Cell type: lymphocyte
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 3
Chemical type: Peptide from protein
Sequence: TMNTKSWLV
Position: 205-213
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Negative
Host: F 3 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 3
Chemical type: Peptide from protein
Sequence: TMNTKSWLV
Position: 205-213
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Negative
Host:  25 days
Cell type: lymphocyte
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 4
Chemical type: Peptide from protein
Sequence: SLKHAARFV
Position: 160-168
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Negative
Host: F 3 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 4
Chemical type: Peptide from protein
Sequence: SLKHAARFV
Position: 160-168
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Negative
Host:  25 days
Cell type: lymphocyte
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 6
Chemical type: Peptide from protein
Sequence: CRVKMQGLK
Position: 288-296
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Negative
Host: F 3 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 6
Chemical type: Peptide from protein
Sequence: CRVKMQGLK
Position: 288-296
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Negative
Host:  25 days
Cell type: lymphocyte
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 8
Chemical type: Peptide from protein
Sequence: QWHRSGSTI
Position: 396-404
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Negative
Host: F 3 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 8
Chemical type: Peptide from protein
Sequence: QWHRSGSTI
Position: 396-404
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Negative
Host:  25 days
Cell type: lymphocyte
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 10
Chemical type: Peptide from protein
Sequence: FGGMSWITQ
Position: 454-462
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Positive
Host: F 3 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 10
Chemical type: Peptide from protein
Sequence: FGGMSWITQ
Position: 454-462
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Positive
Host:  25 days
Cell type: lymphocyte
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 11
Chemical type: Peptide from protein
Sequence: IEFEEAHAT
Position: 240-248
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Positive
Host: F 3 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 11
Chemical type: Peptide from protein
Sequence: IEFEEAHAT
Position: 240-248
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Positive
Host:  25 days
Cell type: lymphocyte
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 12
Chemical type: Peptide from protein
Sequence: LHAALAGAI
Position: 262-270
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Positive
Host: F 3 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 12
Chemical type: Peptide from protein
Sequence: LHAALAGAI
Position: 262-270
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Positive
Host:  25 days
Cell type: lymphocyte
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 13
Chemical type: Peptide from protein
Sequence: YCYEPKVSD
Position: 59-67
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Positive
Host: F 3 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 13
Chemical type: Peptide from protein
Sequence: YCYEPKVSD
Position: 59-67
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Positive
Host:  25 days
Cell type: lymphocyte
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 14
Chemical type: Peptide from protein
Sequence: GMLGALLLWMGLNA
Position: 463-476
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Negative
Host: F 3 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 14
Chemical type: Peptide from protein
Sequence: GMLGALLLWMGLNA
Position: 463-476
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Negative
Host:  25 days
Cell type: lymphocyte
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 15
Chemical type: Peptide from protein
Sequence: VKMMNMEAT
Position: 43-51
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Negative
Host: F 3 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 15
Chemical type: Peptide from protein
Sequence: VKMMNMEAT
Position: 43-51
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Negative
Host:  25 days
Cell type: lymphocyte
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 16
Chemical type: Peptide from protein
Sequence: EWIDVVLEGGSCVTI
Position: 19-33
Source molecule (GenBank): AGL76709.1
Assay category: TCell
Result: Negative
Host: F 3 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 16
Chemical type: Peptide from protein
Sequence: EWIDVVLEGGSCVTI
Position: 19-33
Source molecule (GenBank): AGL76709.1
Assay category: TCell
Result: Negative
Host:  25 days
Cell type: lymphocyte
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 17
Chemical type: Peptide from protein
Sequence: TELAVVRSYCYEPKVSDV
Position: 51-68
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Negative
Host: F 3 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 17
Chemical type: Peptide from protein
Sequence: TELAVVRSYCYEPKVSDV
Position: 51-68
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Negative
Host:  25 days
Cell type: lymphocyte
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 18
Chemical type: Peptide from protein
Sequence: AHNPKATYAEYICKK
Position: 80-94
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Positive
Host: F 3 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 18
Chemical type: Peptide from protein
Sequence: AHNPKATYAEYICKK
Position: 80-94
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Positive
Host:  25 days
Cell type: lymphocyte
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 19
Chemical type: Peptide from protein
Sequence: TYHNYSAQQSLKHAARFVITPKSPVYTA
Position: 151-178
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Positive
Host: F 3 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 19
Chemical type: Peptide from protein
Sequence: TYHNYSAQQSLKHAARFVITPKSPVYTA
Position: 151-178
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Positive
Host:  25 days
Cell type: lymphocyte
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 20
Chemical type: Peptide from protein
Sequence: AHATKQSVVALASQEGALHAALAGAIPVKYSG
Position: 245-276
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Positive
Host: F 3 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 20
Chemical type: Peptide from protein
Sequence: AHATKQSVVALASQEGALHAALAGAIPVKYSG
Position: 245-276
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Positive
Host:  25 days
Cell type: lymphocyte
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 21
Chemical type: Peptide from protein
Sequence: TYPMCSNTFSLVKNPTDTGHGTVVVELSY
Position: 301-329
Source molecule (GenBank): AGL76709.1
Assay category: TCell
Result: Negative
Host: F 3 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 21
Chemical type: Peptide from protein
Sequence: TYPMCSNTFSLVKNPTDTGHGTVVVELSY
Position: 301-329
Source molecule (GenBank): AGL76709.1
Assay category: TCell
Result: Negative
Host:  25 days
Cell type: lymphocyte
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 22
Chemical type: Peptide from protein
Sequence: PVGRLITVNPYVST
Position: 351-364
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Negative
Host: F 3 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 22
Chemical type: Peptide from protein
Sequence: PVGRLITVNPYVST
Position: 351-364
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Negative
Host:  25 days
Cell type: lymphocyte
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 23
Chemical type: Peptide from protein
Sequence: AKIMVEVEPPFGDSFILVG
Position: 369-387
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Negative
Host: F 3 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 23
Chemical type: Peptide from protein
Sequence: AKIMVEVEPPFGDSFILVG
Position: 369-387
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Negative
Host:  25 days
Cell type: lymphocyte
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 24
Chemical type: Peptide from protein
Sequence: SVGGVLTSIGKGIHQVFGSAFKS
Position: 430-452
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Positive
Host: F 3 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 24
Chemical type: Peptide from protein
Sequence: SVGGVLTSIGKGIHQVFGSAFKS
Position: 430-452
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Positive
Host:  25 days
Cell type: lymphocyte
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 25
Chemical type: Peptide from protein
Sequence: NARDRSISM
Position: 475-483
Source molecule (GenBank): AGL76709.1
Assay category: TCell
Result: Negative
Host: F 3 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 25
Chemical type: Peptide from protein
Sequence: NARDRSISM
Position: 475-483
Source molecule (GenBank): AGL76709.1
Assay category: TCell
Result: Negative
Host:  25 days
Cell type: lymphocyte
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 26
Chemical type: Peptide from protein
Sequence: QIRYQWHRSGS
Position: 392-402
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Positive
Host: F 3 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 26
Chemical type: Peptide from protein
Sequence: QIRYQWHRSGS
Position: 392-402
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Positive
Host:  25 days
Cell type: lymphocyte
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 27
Chemical type: Peptide from protein
Sequence: MLGALLLW
Position: 464-471
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Negative
Host: F 3 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 27
Chemical type: Peptide from protein
Sequence: MLGALLLW
Position: 464-471
Source molecule (GenBank): AKL72058.1
Assay category: TCell
Result: Negative
Host:  25 days
Cell type: lymphocyte
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 28
Chemical type: Peptide from protein
Sequence: SMTFLAVGGILVFLA
Position: 482-496
Source molecule (GenBank): AGL76709.1
Assay category: TCell
Result: Negative
Host: F 3 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 28
Chemical type: Peptide from protein
Sequence: SMTFLAVGGILVFLA
Position: 482-496
Source molecule (GenBank): AGL76709.1
Assay category: TCell
Result: Negative
Host:  25 days
Cell type: lymphocyte
Tissue: blood
Culture conditions: Direct Ex Vivo