Reference: Baoyu Liu; Jennifer D Hood; Elizabeth M Kolawole; Derek M Woodruff; Dario A Vignali; Maria Bettini; Brian D Evavold. "A Hybrid Insulin Epitope Maintains High 2D Affinity for Diabetogenic T Cells in the Periphery." Diabetes (2020).

Epitope: Hip2.5
Chemical type: Spliced peptide from proteins
Position: 75-80
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: Hip2.5
Chemical type: Spliced peptide from proteins
Position: 75-80
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: Hip2.5
Chemical type: Spliced peptide from proteins
Position: 75-80
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Positive
Quantitative measurement: 1.8
Host:  5-20 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Hip2.5
Chemical type: Spliced peptide from proteins
Position: 75-80
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Positive
Host:  5-20 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Hip2.5
Chemical type: Spliced peptide from proteins
Position: 75-80
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: Hip2.5
Chemical type: Spliced peptide from proteins
Position: 75-80
Source molecule (GenBank): P01325.1
Assay category: TCell
Result: Positive
Host:  5-20 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Mim1
Chemical type: Peptide, no natural source
Sequence: AHHPIWARMDA
Assay category: TCell
Result: Positive
Host:  5-20 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Mim1
Chemical type: Peptide, no natural source
Sequence: AHHPIWARMDA
Assay category: TCell
Result: Positive
Quantitative measurement: 0.56
Host:  5-20 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Mim1
Chemical type: Peptide, no natural source
Sequence: AHHPIWARMDA
Assay category: TCell
Result: Positive
Host:  5-20 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: ChgA29-42
Chemical type: Peptide from protein
Sequence: DTKVMKCVLEVISD
Position: 29-42
Source molecule (GenBank): P26339.1
Assay category: TCell
Result: Negative
Host:  5-20 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: ChgA29-42
Chemical type: Peptide from protein
Sequence: DTKVMKCVLEVISD
Position: 29-42
Source molecule (GenBank): P26339.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: ChgA29-42
Chemical type: Peptide from protein
Sequence: DTKVMKCVLEVISD
Position: 29-42
Source molecule (GenBank): P26339.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: ChgA29-42
Chemical type: Peptide from protein
Sequence: DTKVMKCVLEVISD
Position: 29-42
Source molecule (GenBank): P26339.1
Assay category: TCell
Result: Positive-Low
Host:  5-20 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: ChgA29-42
Chemical type: Peptide from protein
Sequence: DTKVMKCVLEVISD
Position: 29-42
Source molecule (GenBank): P26339.1
Assay category: TCell
Result: Negative
Host:  5-20 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo