Reference: E Lovegrove; G J Pettigrew; E M Bolton; J A Bradley. "Epitope mapping of the indirect T cell response to allogeneic class I MHC: sequences shared by donor and recipient MHC may prime T cells that provide help for alloantibody production." Journal of immunology (Baltimore, Md. : 1950) (2001).

Epitope: RT1.A<sup>a</sup> P3
Chemical type: Peptide from protein
Sequence: RFIAVGYVDDTEFVR
Position: 45-59
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P3
Chemical type: Peptide from protein
Sequence: RFIAVGYVDDTEFVR
Position: 45-59
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P3
Chemical type: Peptide from protein
Sequence: RFIAVGYVDDTEFVR
Position: 45-59
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P3
Chemical type: Peptide from protein
Sequence: RFIAVGYVDDTEFVR
Position: 45-59
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P4
Chemical type: Peptide from protein
Sequence: TEFVRFDSDAENPRM
Position: 55-69
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P4
Chemical type: Peptide from protein
Sequence: TEFVRFDSDAENPRM
Position: 55-69
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P4
Chemical type: Peptide from protein
Sequence: TEFVRFDSDAENPRM
Position: 55-69
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P4
Chemical type: Peptide from protein
Sequence: TEFVRFDSDAENPRM
Position: 55-69
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P4
Chemical type: Peptide from protein
Sequence: TEFVRFDSDAENPRM
Position: 55-69
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P4
Chemical type: Peptide from protein
Sequence: TEFVRFDSDAENPRM
Position: 55-69
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P5
Chemical type: Peptide from protein
Sequence: ENPRMEPRARWMERE
Position: 65-79
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P5
Chemical type: Peptide from protein
Sequence: ENPRMEPRARWMERE
Position: 65-79
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P5
Chemical type: Peptide from protein
Sequence: ENPRMEPRARWMERE
Position: 65-79
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P5
Chemical type: Peptide from protein
Sequence: ENPRMEPRARWMERE
Position: 65-79
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P6
Chemical type: Peptide from protein
Sequence: WMEREGPEYWEQQTR
Position: 75-89
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P6
Chemical type: Peptide from protein
Sequence: WMEREGPEYWEQQTR
Position: 75-89
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P6
Chemical type: Peptide from protein
Sequence: WMEREGPEYWEQQTR
Position: 75-89
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P6
Chemical type: Peptide from protein
Sequence: WMEREGPEYWEQQTR
Position: 75-89
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P7
Chemical type: Peptide from protein
Sequence: KEWEQIYR
Position: 92-99
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Positive
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P7
Chemical type: Peptide from protein
Sequence: KEWEQIYR
Position: 92-99
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Positive
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P7
Chemical type: Peptide from protein
Sequence: KEWEQIYR
Position: 92-99
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Positive
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P7
Chemical type: Peptide from protein
Sequence: KEWEQIYR
Position: 92-99
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Positive
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P7
Chemical type: Peptide from protein
Sequence: KEWEQIYR
Position: 92-99
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Positive
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P7
Chemical type: Peptide from protein
Sequence: KEWEQIYR
Position: 92-99
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P7
Chemical type: Peptide from protein
Sequence: KEWEQIYR
Position: 92-99
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P8
Chemical type: Peptide from protein
Sequence: EQIYRVDLRTLRGYY
Position: 95-109
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Positive
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P8
Chemical type: Peptide from protein
Sequence: EQIYRVDLRTLRGYY
Position: 95-109
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Positive
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P8
Chemical type: Peptide from protein
Sequence: EQIYRVDLRTLRGYY
Position: 95-109
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Positive
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P8
Chemical type: Peptide from protein
Sequence: EQIYRVDLRTLRGYY
Position: 95-109
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P8
Chemical type: Peptide from protein
Sequence: EQIYRVDLRTLRGYY
Position: 95-109
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Positive-Low
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P8
Chemical type: Peptide from protein
Sequence: EQIYRVDLRTLRGYY
Position: 95-109
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P9
Chemical type: Peptide from protein
Sequence: LRGYYNQSEGGSHTI
Position: 105-119
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Positive
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P9
Chemical type: Peptide from protein
Sequence: LRGYYNQSEGGSHTI
Position: 105-119
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P9
Chemical type: Peptide from protein
Sequence: LRGYYNQSEGGSHTI
Position: 105-119
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P9
Chemical type: Peptide from protein
Sequence: LRGYYNQSEGGSHTI
Position: 105-119
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P10
Chemical type: Peptide from protein
Sequence: GSHTIQEMYGCDVGS
Position: 115-129
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P10
Chemical type: Peptide from protein
Sequence: GSHTIQEMYGCDVGS
Position: 115-129
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P10
Chemical type: Peptide from protein
Sequence: GSHTIQEMYGCDVGS
Position: 115-129
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P10
Chemical type: Peptide from protein
Sequence: GSHTIQEMYGCDVGS
Position: 115-129
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P11
Chemical type: Peptide from protein
Sequence: CDVGSDGSLLRGYRQ
Position: 125-139
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P11
Chemical type: Peptide from protein
Sequence: CDVGSDGSLLRGYRQ
Position: 125-139
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P11
Chemical type: Peptide from protein
Sequence: CDVGSDGSLLRGYRQ
Position: 125-139
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P11
Chemical type: Peptide from protein
Sequence: CDVGSDGSLLRGYRQ
Position: 125-139
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P12
Chemical type: Peptide from protein
Sequence: RGYRQDAYDGRDYIA
Position: 135-149
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P12
Chemical type: Peptide from protein
Sequence: RGYRQDAYDGRDYIA
Position: 135-149
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P12
Chemical type: Peptide from protein
Sequence: RGYRQDAYDGRDYIA
Position: 135-149
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P12
Chemical type: Peptide from protein
Sequence: RGYRQDAYDGRDYIA
Position: 135-149
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P12
Chemical type: Peptide from protein
Sequence: RGYRQDAYDGRDYIA
Position: 135-149
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P12
Chemical type: Peptide from protein
Sequence: RGYRQDAYDGRDYIA
Position: 135-149
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P13
Chemical type: Peptide from protein
Sequence: RDYIALNEDLKTWTA
Position: 145-159
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P13
Chemical type: Peptide from protein
Sequence: RDYIALNEDLKTWTA
Position: 145-159
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P13
Chemical type: Peptide from protein
Sequence: RDYIALNEDLKTWTA
Position: 145-159
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P13
Chemical type: Peptide from protein
Sequence: RDYIALNEDLKTWTA
Position: 145-159
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P14
Chemical type: Peptide from protein
Sequence: KTWTAADFAAQITRN
Position: 155-169
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P14
Chemical type: Peptide from protein
Sequence: KTWTAADFAAQITRN
Position: 155-169
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P14
Chemical type: Peptide from protein
Sequence: KTWTAADFAAQITRN
Position: 155-169
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P14
Chemical type: Peptide from protein
Sequence: KTWTAADFAAQITRN
Position: 155-169
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P15
Chemical type: Peptide from protein
Sequence: QITRNKWERARYAER
Position: 165-179
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P15
Chemical type: Peptide from protein
Sequence: QITRNKWERARYAER
Position: 165-179
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P15
Chemical type: Peptide from protein
Sequence: QITRNKWERARYAER
Position: 165-179
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P15
Chemical type: Peptide from protein
Sequence: QITRNKWERARYAER
Position: 165-179
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P15
Chemical type: Peptide from protein
Sequence: QITRNKWERARYAER
Position: 165-179
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P16
Chemical type: Peptide from protein
Sequence: RYAERLRAYLEGTCV
Position: 175-189
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P16
Chemical type: Peptide from protein
Sequence: RYAERLRAYLEGTCV
Position: 175-189
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Positive
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P16
Chemical type: Peptide from protein
Sequence: RYAERLRAYLEGTCV
Position: 175-189
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P16
Chemical type: Peptide from protein
Sequence: RYAERLRAYLEGTCV
Position: 175-189
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P17
Chemical type: Peptide from protein
Sequence: EGTCVEWLSRYLELG
Position: 185-199
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P17
Chemical type: Peptide from protein
Sequence: EGTCVEWLSRYLELG
Position: 185-199
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P17
Chemical type: Peptide from protein
Sequence: EGTCVEWLSRYLELG
Position: 185-199
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P17
Chemical type: Peptide from protein
Sequence: EGTCVEWLSRYLELG
Position: 185-199
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P18
Chemical type: Peptide from protein
Sequence: YLELGKETLLRSDPP
Position: 195-209
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P18
Chemical type: Peptide from protein
Sequence: YLELGKETLLRSDPP
Position: 195-209
Source molecule (GenBank): P16391.2
Assay category: BCell
Result: Negative
Host:  8-12 weeks

Epitope: RT1.A<sup>a</sup> P18
Chemical type: Peptide from protein
Sequence: YLELGKETLLRSDPP
Position: 195-209
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: RT1.A<sup>a</sup> P18
Chemical type: Peptide from protein
Sequence: YLELGKETLLRSDPP
Position: 195-209
Source molecule (GenBank): P16391.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo