Reference: Kerstin Dell; Robert Koesters; Lutz Gissmann. "Transcutaneous immunization in mice: induction of T-helper and cytotoxic T lymphocyte responses and protection against human papillomavirus-induced tumors." International journal of cancer. Journal international du cancer (2006).

Epitope: OVA<sub>257-264</sub>
Chemical type: Peptide from protein
Sequence: SIINFEKL
Position: 258-265
Source molecule (GenBank): AAA48998.1
Assay category: TCell
Result: Positive-Low
Quantitative measurement: 25.0
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: OVA<sub>257-264</sub>
Chemical type: Peptide from protein
Sequence: SIINFEKL
Position: 258-265
Source molecule (GenBank): AAA48998.1
Assay category: TCell
Result: Positive-High
Quantitative measurement: 100.0
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: OVA<sub>265-280</sub>
Chemical type: Peptide from protein
Sequence: TEWTSSNVMEERKIKV
Position: 266-281
Source molecule (GenBank): AAA48998.1
Assay category: TCell
Result: Positive-High
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: OVA<sub>265-280</sub>
Chemical type: Peptide from protein
Sequence: TEWTSSNVMEERKIKV
Position: 266-281
Source molecule (GenBank): AAA48998.1
Assay category: TCell
Result: Positive-Low
Quantitative measurement: 5.0
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: OVA<sub>265-280</sub>
Chemical type: Peptide from protein
Sequence: TEWTSSNVMEERKIKV
Position: 266-281
Source molecule (GenBank): AAA48998.1
Assay category: TCell
Result: Positive-High
Quantitative measurement: 60.0
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: OVA<sub>265-280</sub>
Chemical type: Peptide from protein
Sequence: TEWTSSNVMEERKIKV
Position: 266-281
Source molecule (GenBank): AAA48998.1
Assay category: TCell
Result: Positive-Low
Quantitative measurement: 1.0
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: OVA<sub>265-280</sub>
Chemical type: Peptide from protein
Sequence: TEWTSSNVMEERKIKV
Position: 266-281
Source molecule (GenBank): AAA48998.1
Assay category: TCell
Result: Positive-High
Quantitative measurement: 28.0
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: OVA<sub>265-280</sub>
Chemical type: Peptide from protein
Sequence: TEWTSSNVMEERKIKV
Position: 266-281
Source molecule (GenBank): AAA48998.1
Assay category: TCell
Result: Positive-Low
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: OVA<sub>257-280</sub>
Chemical type: Peptide from protein
Sequence: SIINFEKLTEWTSSNVMEERKIKV
Position: 258-281
Source molecule (GenBank): AAA48998.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: E7<sub>49-57</sub>
Chemical type: Peptide from protein
Sequence: RAHYNIVTF
Position: 49-57
Source molecule (GenBank): ABL96585.1
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: E7<sub>49-57</sub>
Chemical type: Peptide from protein
Sequence: RAHYNIVTF
Position: 49-57
Source molecule (GenBank): ABL96585.1
Assay category: TCell
Result: Positive
Quantitative measurement: 1000.0
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E7<sub>49-57</sub>
Chemical type: Peptide from protein
Sequence: RAHYNIVTF
Position: 49-57
Source molecule (GenBank): ABL96585.1
Assay category: TCell
Result: Positive-Low
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E7<sub>44-62</sub>
Chemical type: Peptide from protein
Sequence: QAEPDRAHYNIVTFCCKCD
Position: 44-62
Source molecule (GenBank): ABL96585.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks

Epitope: E7<sub>44-62</sub>
Chemical type: Peptide from protein
Sequence: QAEPDRAHYNIVTFCCKCD
Position: 44-62
Source molecule (GenBank): ABL96585.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: E7<sub>44-62</sub>
Chemical type: Peptide from protein
Sequence: QAEPDRAHYNIVTFCCKCD
Position: 44-62
Source molecule (GenBank): ABL96585.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated