Reference: Antonino Cassotta; Vincent Mikol; Thomas Bertrand; Stéphanie Pouzieux; Josiane Le Parc; Paul Ferrari; Jacques Dumas; Michael Auer; Florian Deisenhammer; Matteo Gastaldi; Diego Franciotta; Chiara Silacci-Fregni; Blanca Fernandez Rodriguez; Isabella Giacchetto-Sasselli; Mathilde Foglierini; David Jarrossay; Roger Geiger; Federica Sallusto; Antonio Lanzavecchia; Luca Piccoli. "A single T cell epitope drives the neutralizing anti-drug antibody response to natalizumab in multiple sclerosis patients." Nature medicine (2019).

Epitope: Epitope of Fab NAA32 on natalizumab
Chemical type: Region on multi-chain protein
Discontinuous residues: H: K30, D31, T32, Y33, D52, A54, N55, Y57, K59, Y101, G103, N104, Y105, G106, V107, Y108; L: Q27, N30, Y32, Y50, D92, N93
Source molecule (GenBank): 4IRZ_H
Assay category: BCell
Result: Positive
Quantitative measurement: 2.03
Host: F

Epitope: Epitope of Fab NAA32 on natalizumab
Chemical type: Region on multi-chain protein
Discontinuous residues: H: K30, D31, T32, Y33, D52, A54, N55, Y57, K59, Y101, G103, N104, Y105, G106, V107, Y108; L: Q27, N30, Y32, Y50, D92, N93
Source molecule (GenBank): 4IRZ_H
Assay category: BCell
Result: Negative
Host: F

Epitope: Epitope of Fab NAA32 on natalizumab
Chemical type: Region on multi-chain protein
Discontinuous residues: H: K30, D31, T32, Y33, D52, A54, N55, Y57, K59, Y101, G103, N104, Y105, G106, V107, Y108; L: Q27, N30, Y32, Y50, D92, N93
Source molecule (GenBank): 4IRZ_H
Assay category: BCell
Result: Positive
Quantitative measurement: 0.9
Host: F

Epitope: Epitope of Fab NAA32 on natalizumab
Chemical type: Region on multi-chain protein
Discontinuous residues: H: K30, D31, T32, Y33, D52, A54, N55, Y57, K59, Y101, G103, N104, Y105, G106, V107, Y108; L: Q27, N30, Y32, Y50, D92, N93
Source molecule (GenBank): 4IRZ_H
Assay category: BCell
Result: Positive
Quantitative measurement: 1200000.0
Host: F

Epitope: Epitope of Fab NAA32 on natalizumab
Chemical type: Region on multi-chain protein
Discontinuous residues: H: K30, D31, T32, Y33, D52, A54, N55, Y57, K59, Y101, G103, N104, Y105, G106, V107, Y108; L: Q27, N30, Y32, Y50, D92, N93
Source molecule (GenBank): 4IRZ_H
Assay category: BCell
Result: Positive
Quantitative measurement: 0.0010
Host: F

Epitope: Epitope of Fab NAA32 on natalizumab
Chemical type: Region on multi-chain protein
Discontinuous residues: H: K30, D31, T32, Y33, D52, A54, N55, Y57, K59, Y101, G103, N104, Y105, G106, V107, Y108; L: Q27, N30, Y32, Y50, D92, N93
Source molecule (GenBank): 4IRZ_H
Assay category: BCell
Result: Positive
Host: F

Epitope: Epitope of Fab NAA84 on natalizumab
Chemical type: Region on multi-chain protein
Discontinuous residues: H: K30, D31, T32, Y33, R50, D52, A54, N55, K59, E99, Y101, G106, Y108; L: N30, Y32, Y91, D92, N93, L94, W95
Source molecule (GenBank): 4IRZ_H
Assay category: BCell
Result: Positive
Quantitative measurement: 0.0000084
Host: F

Epitope: Epitope of Fab NAA84 on natalizumab
Chemical type: Region on multi-chain protein
Discontinuous residues: H: K30, D31, T32, Y33, R50, D52, A54, N55, K59, E99, Y101, G106, Y108; L: N30, Y32, Y91, D92, N93, L94, W95
Source molecule (GenBank): 4IRZ_H
Assay category: BCell
Result: Positive
Quantitative measurement: 760000.0
Host: F

Epitope: Epitope of Fab NAA84 on natalizumab
Chemical type: Region on multi-chain protein
Discontinuous residues: H: K30, D31, T32, Y33, R50, D52, A54, N55, K59, E99, Y101, G106, Y108; L: N30, Y32, Y91, D92, N93, L94, W95
Source molecule (GenBank): 4IRZ_H
Assay category: BCell
Result: Positive
Quantitative measurement: 0.011
Host: F

Epitope: Epitope of Fab NAA84 on natalizumab
Chemical type: Region on multi-chain protein
Discontinuous residues: H: K30, D31, T32, Y33, R50, D52, A54, N55, K59, E99, Y101, G106, Y108; L: N30, Y32, Y91, D92, N93, L94, W95
Source molecule (GenBank): 4IRZ_H
Assay category: BCell
Result: Positive
Quantitative measurement: 2.788
Host: F

Epitope: Epitope of Fab NAA84 on natalizumab
Chemical type: Region on multi-chain protein
Discontinuous residues: H: K30, D31, T32, Y33, R50, D52, A54, N55, K59, E99, Y101, G106, Y108; L: N30, Y32, Y91, D92, N93, L94, W95
Source molecule (GenBank): 4IRZ_H
Assay category: BCell
Result: Positive
Host: F

Epitope: Epitope of Fab NAA84 on natalizumab
Chemical type: Region on multi-chain protein
Discontinuous residues: H: K30, D31, T32, Y33, R50, D52, A54, N55, K59, E99, Y101, G106, Y108; L: N30, Y32, Y91, D92, N93, L94, W95
Source molecule (GenBank): 4IRZ_H
Assay category: BCell
Result: Positive
Host: F

Epitope: DTYIHWVRQAPGQR
Chemical type: Peptide from protein
Sequence: DTYIHWVRQAPGQR
Position: 31-44
Source molecule (GenBank): 4IRZ_H
Assay category: MhcLigandElution
Result: Positive

Epitope: DTSASTAYMELSSLRSEDT
Chemical type: Peptide from protein
Sequence: DTSASTAYMELSSLRSEDT
Position: 73-91
Source molecule (GenBank): 6FG1_A
Assay category: MhcLigandElution
Result: Positive

Epitope: ASTAYMELSSLRSEDT
Chemical type: Peptide from protein
Sequence: ASTAYMELSSLRSEDT
Position: 76-91
Source molecule (GenBank): 6FG1_A
Assay category: MhcLigandElution
Result: Positive

Epitope: ASTAYMELSSLRSEDTA
Chemical type: Peptide from protein
Sequence: ASTAYMELSSLRSEDTA
Position: 76-92
Source molecule (GenBank): 6FG1_A
Assay category: MhcLigandElution
Result: Positive

Epitope: STAYMELSSLRSED
Chemical type: Peptide from protein
Sequence: STAYMELSSLRSED
Position: 77-90
Source molecule (GenBank): 4IRZ_H
Assay category: MhcLigandElution
Result: Positive

Epitope: STAYMELSSLRSEDT
Chemical type: Peptide from protein
Sequence: STAYMELSSLRSEDT
Position: 77-91
Source molecule (GenBank): 6FG1_A
Assay category: MhcLigandElution
Result: Positive

Epitope: STAYMELSSLRSEDTA
Chemical type: Peptide from protein
Sequence: STAYMELSSLRSEDTA
Position: 77-92
Source molecule (GenBank): 6FG1_A
Assay category: MhcLigandElution
Result: Positive

Epitope: TAYMELSSLRSEDT
Chemical type: Peptide from protein
Sequence: TAYMELSSLRSEDT
Position: 78-91
Source molecule (GenBank): 6FG1_A
Assay category: MhcLigandElution
Result: Positive

Epitope: GQGTLVTVSSASTKGP
Chemical type: Peptide from protein
Sequence: GQGTLVTVSSASTKGP
Position: 114-129
Source molecule (GenBank): 4IRZ_H
Assay category: MhcLigandElution
Result: Positive

Epitope: GQGTLVTVSSASTKGPS
Chemical type: Peptide from protein
Sequence: GQGTLVTVSSASTKGPS
Position: 114-130
Source molecule (GenBank): 4IRZ_H
Assay category: MhcLigandElution
Result: Positive

Epitope: QGTLVTVSSASTKGP
Chemical type: Peptide from protein
Sequence: QGTLVTVSSASTKGP
Position: 115-129
Source molecule (GenBank): 4IRZ_H
Assay category: MhcLigandElution
Result: Positive

Epitope: QGTLVTVSSASTKGPS
Chemical type: Peptide from protein
Sequence: QGTLVTVSSASTKGPS
Position: 115-130
Source molecule (GenBank): 4IRZ_H
Assay category: MhcLigandElution
Result: Positive

Epitope: TPGKAPRLLIHYTSALQPG
Chemical type: Peptide from protein
Sequence: TPGKAPRLLIHYTSALQPG
Position: 39-57
Source molecule (GenBank): 4IRZ_L
Assay category: MhcLigandElution
Result: Positive

Epitope: TPGKAPRLLIHYTSALQPGIPS
Chemical type: Peptide from protein
Sequence: TPGKAPRLLIHYTSALQPGIPS
Position: 39-60
Source molecule (GenBank): 4IRZ_L
Assay category: MhcLigandElution
Result: Positive

Epitope: TPGKAPRLLIHYTSALQPGIPSR
Chemical type: Peptide from protein
Sequence: TPGKAPRLLIHYTSALQPGIPSR
Position: 39-61
Source molecule (GenBank): 4IRZ_L
Assay category: MhcLigandElution
Result: Positive

Epitope: KAPRLLIHYTSALQPG
Chemical type: Peptide from protein
Sequence: KAPRLLIHYTSALQPG
Position: 42-57
Source molecule (GenBank): 4IRZ_L
Assay category: MhcLigandElution
Result: Positive

Epitope: APRLLIHYTSALQPG
Chemical type: Peptide from protein
Sequence: KAPRLLIHYTSALQPG
Position: 42-57
Source molecule (GenBank): 4IRZ_L
Assay category: MhcLigandElution
Result: Positive

Epitope: APRLLIHYTSALQPGIPS
Chemical type: Peptide from protein
Sequence: APRLLIHYTSALQPGIPS
Position: 43-60
Source molecule (GenBank): 4IRZ_L
Assay category: MhcLigandElution
Result: Positive

Epitope: NZM-LC (FR2-CDR2)
Chemical type: Peptide from protein
Sequence: GKAPRLLIHYTSALQPGI
Position: 41-58
Source molecule (GenBank): 4IRZ_L
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: NZM-LC (FR2-CDR2)
Chemical type: Peptide from protein
Sequence: GKAPRLLIHYTSALQPGI
Position: 41-58
Source molecule (GenBank): 4IRZ_L
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: NZM-LC (FR2-CDR2)
Chemical type: Peptide from protein
Sequence: GKAPRLLIHYTSALQPGI
Position: 41-58
Source molecule (GenBank): 4IRZ_L
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: NZM-LC (FR2-CDR2)
Chemical type: Peptide from protein
Sequence: GKAPRLLIHYTSALQPGI
Position: 41-58
Source molecule (GenBank): 4IRZ_L
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: NZM-LC (FR2-CDR2) (41-55)
Chemical type: Peptide from protein
Sequence: GKAPRLLIHYTSALQ
Position: 41-55
Source molecule (GenBank): 4IRZ_L
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: NZM-LC (FR2-CDR2) (41-60)
Chemical type: Peptide from protein
Sequence: GKAPRLLIHYTSALQPGIPS
Position: 41-60
Source molecule (GenBank): 4IRZ_L
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: NZM-LC (FR2-CDR2) (31-45)
Chemical type: Peptide from protein
Sequence: KYMAWYQQTPGKAPR
Position: 31-45
Source molecule (GenBank): 4IRZ_L
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: NZM-LC (FR2-CDR2) (31-45)
Chemical type: Peptide from protein
Sequence: KYMAWYQQTPGKAPR
Position: 31-45
Source molecule (GenBank): 4IRZ_L
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: NZM-HC (41-55)
Chemical type: Peptide from protein
Sequence: PGQRLEWMGRIDPAN
Position: 41-55
Source molecule (GenBank): 4IRZ_H
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: NZM-LC (FR2-CDR2) (44-58)
Chemical type: Peptide from protein
Sequence: PRLLIHYTSALQPGI
Position: 44-58
Source molecule (GenBank): 4IRZ_L
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone