Reference: Giacomo Oliveira; Kari Stromhaug; Nicoletta Cieri; J Bryan Iorgulescu; Susan Klaeger; Jacquelyn O Wolff; Suzanna Rachimi; Vipheaviny Chea; Kate Krause; Samuel S Freeman; Wandi Zhang; Shuqiang Li; David A Braun; Donna Neuberg; Steven A Carr; Kenneth J Livak; Dennie T Frederick; Edward F Fritsch; Megan Wind-Rotolo; Nir Hacohen; Moshe Sade-Feldman; Charles H Yoon; Derin B Keskin; Patrick A Ott; Scott J Rodig; Genevieve M Boland; Catherine J Wu. "Landscape of helper and regulatory antitumour CD4<sup>+</sup> T cells in melanoma." Nature (2022).

Epitope: RPS9 p.P68S
Chemical type: Peptide, no natural source
Sequence: AARELLTLDEKDSRRLFE
Assay category: TCell
Result: Positive
Host: M 61
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Engineered

Epitope: RPS9 56-73
Chemical type: Peptide from protein
Sequence: AARELLTLDEKDPRRLFE
Position: 56-73
Source molecule (GenBank): C9JM19.1
Assay category: TCell
Result: Positive-Low
Host: M 61
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Engineered

Epitope: MDH2 p.P214L
Chemical type: Peptide, no natural source
Sequence: EEKMISDAILELKAS
Assay category: TCell
Result: Negative
Host: M 61
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Engineered

Epitope: MDH2 p.P214L
Chemical type: Peptide, no natural source
Sequence: EEKMISDAILELKAS
Assay category: TCell
Result: Positive
Host: M 61
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Engineered

Epitope: DST p.S5447F
Chemical type: Peptide, no natural source
Sequence: LLQRLLDDRKFTVEV
Assay category: TCell
Result: Negative
Host: M 61
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Engineered

Epitope: DST p.S5447F
Chemical type: Peptide, no natural source
Sequence: LLQRLLDDRKFTVEV
Assay category: TCell
Result: Positive
Host: M 61
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Engineered

Epitope: LAMC1p.S1509F
Chemical type: Peptide, no natural source
Sequence: KAKNFVTSLLSIIND
Assay category: TCell
Result: Positive
Host: M 61
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Engineered

Epitope: PPP3CB p.188_189ME>IK
Chemical type: Peptide, no natural source
Sequence: KYSERVYEACIKAFD
Assay category: TCell
Result: Negative
Host: M 61
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Engineered

Epitope: PPP3CB p.188_189ME>IK
Chemical type: Peptide, no natural source
Sequence: KYSERVYEACIKAFD
Assay category: TCell
Result: Positive
Host: M 61
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Engineered

Epitope: EDIL3 p.P298F
Chemical type: Peptide, no natural source
Sequence: KAQYVRLYFQVCRRH
Assay category: TCell
Result: Positive
Host: F 63
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Engineered

Epitope: PLEC 3278-3292
Chemical type: Peptide from protein
Sequence: GGIVDPSKSHRVPLD
Position: 3047-3061
Source molecule (GenBank): A0A8I5KSD5.1
Assay category: TCell
Result: Positive
Host: F 63
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Engineered

Epitope: NCOA5 p.R91C
Chemical type: Peptide, no natural source
Sequence: VRDLRDFCDLRDSRD
Assay category: TCell
Result: Negative
Host: F 63
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Engineered

Epitope: NCOA5 p.R91C
Chemical type: Peptide, no natural source
Sequence: VRDLRDFCDLRDSRD
Assay category: TCell
Result: Positive
Host: F 63
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Engineered

Epitope: ATXN2L p.P758L
Chemical type: Peptide, no natural source
Sequence: KGSLLPQRSDQHQPA
Assay category: TCell
Result: Positive
Host: F 63
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Engineered

Epitope: ATXN2L 754-768
Chemical type: Peptide from protein
Sequence: KGSLPPQRSDQHQPA
Position: 73-87
Source molecule (GenBank): H3BRB0.2
Assay category: TCell
Result: Positive
Host: F 63
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Engineered

Epitope: ATXN2L p.P758L
Chemical type: Peptide, no natural source
Sequence: QGKYRGAKGSLLPQR
Assay category: TCell
Result: Positive
Host: F 63
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Engineered

Epitope: ATXN2L 747-761
Chemical type: Peptide from protein
Sequence: QGKYRGAKGSLPPQR
Position: 66-80
Source molecule (GenBank): H3BRB0.2
Assay category: TCell
Result: Positive
Host: F 63
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Engineered

Epitope: POLR1B p.F378I
Chemical type: Peptide, no natural source
Sequence: TKPFEYLIATGNLRS
Assay category: TCell
Result: Positive
Host: F 63
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Engineered

Epitope: POLR1B 371-385
Chemical type: Peptide from protein
Sequence: TKPFEYLFATGNLRS
Position: 427-441
Source molecule (GenBank): Q9H9Y6.2
Assay category: TCell
Result: Positive-Low
Host: F 63
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Engineered

Epitope: PLXNB2 1743-1757
Chemical type: Peptide from protein
Sequence: KEISTYKKMVEDYYK
Position: 1743-1757
Source molecule (GenBank): O15031.3
Assay category: TCell
Result: Positive-Low
Host: F 63
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Engineered

Epitope: PDIA6 p.P456S
Chemical type: Peptide, no natural source
Sequence: APVGGGAFSTIVEREPWDG
Assay category: TCell
Result: Positive
Host: F 63
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Engineered

Epitope: PDIA6 p.P456S
Chemical type: Peptide, no natural source
Sequence: APVGGGAFSTIVEREPWDG
Assay category: TCell
Result: Negative
Host: F 63
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Engineered

Epitope: EDIL3 290-304
Chemical type: Peptide from protein
Sequence: KAQYVRLYPQVCRRH
Position: 290-304
Source molecule (GenBank): O43854.1
Assay category: TCell
Result: Positive
Host: F 63
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Engineered