Reference: David C Tscharke; Wai-Ping Woo; Isaac G Sakala; John Sidney; Alessandro Sette; Denis J Moss; Jack R Bennink; Gunasegaran Karupiah; Jonathan W Yewdell. "Poxvirus CD8+ T-cell determinants and cross-reactivity in BALB/c mice." Journal of virology (2006).

Epitope: A52<sub>75-83</sub>
Chemical type: Peptide from protein
Sequence: KYGRLFNEI
Position: 75-83
Source molecule (GenBank): Q01220.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A52<sub>75-83</sub>
Chemical type: Peptide from protein
Sequence: KYGRLFNEI
Position: 75-83
Source molecule (GenBank): Q01220.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A52<sub>75-83</sub>
Chemical type: Peptide from protein
Sequence: KYGRLFNEI
Position: 75-83
Source molecule (GenBank): Q01220.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A52<sub>75-83</sub>
Chemical type: Peptide from protein
Sequence: KYGRLFNEI
Position: 75-83
Source molecule (GenBank): Q01220.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 0.5

Epitope: F2<sub>26-34</sub>
Chemical type: Peptide from protein
Sequence: SPYAAGYDL
Position: 26-34
Source molecule (GenBank): AAO89320.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: F2<sub>26-34</sub>
Chemical type: Peptide from protein
Sequence: SPYAAGYDL
Position: 26-34
Source molecule (GenBank): AAO89320.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: F2<sub>26-34</sub>
Chemical type: Peptide from protein
Sequence: SPYAAGYDL
Position: 26-34
Source molecule (GenBank): AAO89320.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Cell Line / Clone

Epitope: F2<sub>26-34</sub>
Chemical type: Peptide from protein
Sequence: SPYAAGYDL
Position: 26-34
Source molecule (GenBank): AAO89320.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Cell Line / Clone

Epitope: F2<sub>26-34</sub>
Chemical type: Peptide from protein
Sequence: SPYAAGYDL
Position: 26-34
Source molecule (GenBank): AAO89320.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: F2<sub>26-34</sub>
Chemical type: Peptide from protein
Sequence: SPYAAGYDL
Position: 26-34
Source molecule (GenBank): AAO89320.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Cell Line / Clone

Epitope: F2<sub>26-34</sub>
Chemical type: Peptide from protein
Sequence: SPYAAGYDL
Position: 26-34
Source molecule (GenBank): AAO89320.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Cell Line / Clone

Epitope: F2<sub>26-34</sub>
Chemical type: Peptide from protein
Sequence: SPYAAGYDL
Position: 26-34
Source molecule (GenBank): AAO89320.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 0.5

Epitope: E3<sub>140-148</sub>
Chemical type: Peptide from protein
Sequence: VGPSNSPTF
Position: 140-148
Source molecule (GenBank): P21605.1
Assay category: TCell
Result: Positive-High
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E3<sub>140-148</sub>
Chemical type: Peptide from protein
Sequence: VGPSNSPTF
Position: 140-148
Source molecule (GenBank): P21605.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E3<sub>140-148</sub>
Chemical type: Peptide from protein
Sequence: VGPSNSPTF
Position: 140-148
Source molecule (GenBank): P21605.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E3<sub>140-148</sub>
Chemical type: Peptide from protein
Sequence: VGPSNSPTF
Position: 140-148
Source molecule (GenBank): P21605.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E3<sub>140-148</sub>
Chemical type: Peptide from protein
Sequence: VGPSNSPTF
Position: 140-148
Source molecule (GenBank): P21605.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E3<sub>140-148</sub>
Chemical type: Peptide from protein
Sequence: VGPSNSPTF
Position: 140-148
Source molecule (GenBank): P21605.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 0.5

Epitope: F2(G)
Chemical type: Peptide from protein
Sequence: SPGAAGYDL
Position: 26-34
Source molecule (GenBank): Q76RE7.1
Assay category: TCell
Result: Positive-Intermediate
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: F2(G)
Chemical type: Peptide from protein
Sequence: SPGAAGYDL
Position: 26-34
Source molecule (GenBank): Q76RE7.1
Assay category: TCell
Result: Positive-Intermediate
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: F2(G)
Chemical type: Peptide from protein
Sequence: SPGAAGYDL
Position: 26-34
Source molecule (GenBank): Q76RE7.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: F2(G)
Chemical type: Peptide from protein
Sequence: SPGAAGYDL
Position: 26-34
Source molecule (GenBank): Q76RE7.1
Assay category: TCell
Result: Positive-Intermediate
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Cell Line / Clone

Epitope: F2(G)
Chemical type: Peptide from protein
Sequence: SPGAAGYDL
Position: 26-34
Source molecule (GenBank): Q76RE7.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 3.7

Epitope: F2(NH)
Chemical type: Peptide from protein
Sequence: SNHAAGYDL
Position: 26-34
Source molecule (GenBank): AAM92330.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Cell Line / Clone

Epitope: F2(NH)
Chemical type: Peptide from protein
Sequence: SNHAAGYDL
Position: 26-34
Source molecule (GenBank): AAM92330.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: F2(NH)
Chemical type: Peptide from protein
Sequence: SNHAAGYDL
Position: 26-34
Source molecule (GenBank): AAM92330.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: F2(NH)
Chemical type: Peptide from protein
Sequence: SNHAAGYDL
Position: 26-34
Source molecule (GenBank): AAM92330.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Cell Line / Clone

Epitope: F2(NH)
Chemical type: Peptide from protein
Sequence: SNHAAGYDL
Position: 26-34
Source molecule (GenBank): AAM92330.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: F2(NH)
Chemical type: Peptide from protein
Sequence: SNHAAGYDL
Position: 26-34
Source molecule (GenBank): AAM92330.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 5.3

Epitope: A52<sub>117-126</sub>
Chemical type: Peptide from protein
Sequence: DYIKVQKQDI
Position: 117-126
Source molecule (GenBank): Q01220.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 80.0

Epitope: A52<sub>158-166</sub>
Chemical type: Peptide from protein
Sequence: KYCLRAIKL
Position: 158-166
Source molecule (GenBank): Q01220.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 187.0

Epitope: A52<sub>153-161</sub>
Chemical type: Peptide from protein
Sequence: LFSRWKYCL
Position: 153-161
Source molecule (GenBank): Q01220.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 5002.0

Epitope: F2<sub>23-32</sub>
Chemical type: Peptide from protein
Sequence: SPYAAGYDLY
Position: 23-32
Source molecule (GenBank): P17374.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 230.0

Epitope: F2<sub>55-63</sub>
Chemical type: Peptide from protein
Sequence: MPKFCYGRI
Position: 55-63
Source molecule (GenBank): P17374.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 256.0

Epitope: F2<sub>39-47</sub>
Chemical type: Peptide from protein
Sequence: IPPGERQLI
Position: 39-47
Source molecule (GenBank): P17374.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 543.0

Epitope: F2<sub>18-26</sub>
Chemical type: Peptide from protein
Sequence: SPTRQSPYA
Position: 18-26
Source molecule (GenBank): P17374.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 1922.0

Epitope: E3<sub>78-85</sub>
Chemical type: Peptide from protein
Sequence: DAMADVII
Position: 78-85
Source molecule (GenBank): P21605.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 267.0

Epitope: E3<sub>16-24</sub>
Chemical type: Peptide from protein
Sequence: CEAIKTIGI
Position: 16-24
Source molecule (GenBank): P21605.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 543.0

Epitope: E3<sub>86-94</sub>
Chemical type: Peptide from protein
Sequence: DDVSREKSM
Position: 86-94
Source molecule (GenBank): P21605.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 651.0