Reference: Yifeng Qin; Kaihang Tu; Qingyuan Teng; Delan Feng; Ye Zhao; Guozhong Zhang. "Identification of Novel T-Cell Epitopes on Infectious Bronchitis Virus N Protein and Development of a Multi-epitope Vaccine." Journal of virology (2021).

Epitope: N(1-20)
Chemical type: Peptide from protein
Sequence: MASGKVSGKSDSPAPIIKLG
Position: 1-20
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(11-30)
Chemical type: Peptide from protein
Sequence: DSPAPIIKLGGPKPPKVGSS
Position: 11-30
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(21-40)
Chemical type: Peptide from protein
Sequence: GPKPPKVGSSGNASWFQAIK
Position: 21-40
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(31-50)
Chemical type: Peptide from protein
Sequence: GNASWFQAIKAKKLNAPAPK
Position: 31-50
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(41-60)
Chemical type: Peptide from protein
Sequence: AKKLNAPAPKFEGSGVPDNE
Position: 41-60
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(51-70)
Chemical type: Peptide from protein
Sequence: FEGSGVPDNENLKNSQQHGY
Position: 51-70
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(61-80)
Chemical type: Peptide from protein
Sequence: NLKNSQQHGYWRRQARYKPG
Position: 61-80
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(71-90)
Chemical type: Peptide from protein
Sequence: WRRQARYKPGKGGRKPVPDA
Position: 71-90
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(81-100)
Chemical type: Peptide from protein
Sequence: KGGRKPVPDAWYFYYTGTGP
Position: 81-100
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(91-110)
Chemical type: Peptide from protein
Sequence: WYFYYTGTGPAADLNWGDSQ
Position: 91-110
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(101-120)
Chemical type: Peptide from protein
Sequence: AADLNWGDSQDGIVWVAAKG
Position: 101-120
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(111-130)
Chemical type: Peptide from protein
Sequence: DGIVWVAAKGADVKSRSYQG
Position: 111-130
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(121-140)
Chemical type: Peptide from protein
Sequence: ADVKSRSYQGTRDPDKFDQY
Position: 121-140
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(131-150)
Chemical type: Peptide from protein
Sequence: TRDPDKFDQYPLRFSDGGPD
Position: 131-150
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(141-160)
Chemical type: Peptide from protein
Sequence: PLRFSDGGPDGNFRWDFIPL
Position: 141-160
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(151-170)
Chemical type: Peptide from protein
Sequence: GNFRWDFIPLNRGRSGRSTA
Position: 151-170
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(161-180)
Chemical type: Peptide from protein
Sequence: NRGRSGRSTAASSAASSRAP
Position: 161-180
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(171-190)
Chemical type: Peptide from protein
Sequence: ASSAASSRAPSREGSRGRRS
Position: 171-190
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(191-210)
Chemical type: Peptide from protein
Sequence: GAEDDLIARAAKIIQDQQRK
Position: 191-210
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(201-220)
Chemical type: Peptide from protein
Sequence: AKIIQDQQRKGTRITKQKAD
Position: 201-220
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(211-100)
Chemical type: Peptide from protein
Sequence: GTRITKQKADEMAHRRFCKR
Position: 211-230
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(211-100)
Chemical type: Peptide from protein
Sequence: GTRITKQKADEMAHRRFCKR
Position: 211-230
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(211-100)
Chemical type: Peptide from protein
Sequence: GTRITKQKADEMAHRRFCKR
Position: 211-230
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Positive
Host:  2 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(211-100)
Chemical type: Peptide from protein
Sequence: GTRITKQKADEMAHRRFCKR
Position: 211-230
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(211-100)
Chemical type: Peptide from protein
Sequence: GTRITKQKADEMAHRRFCKR
Position: 211-230
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Positive
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(211-100)
Chemical type: Peptide from protein
Sequence: GTRITKQKADEMAHRRFCKR
Position: 211-230
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Positive
Host:  2 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(221-240)
Chemical type: Peptide from protein
Sequence: EMAHRRFCKRTIPPGYRVDQ
Position: 221-240
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(231-250)
Chemical type: Peptide from protein
Sequence: TIPPGYRVDQVFGPRTKGKE
Position: 231-250
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(241-260)
Chemical type: Peptide from protein
Sequence: VFGPRTKGKEGNFGDDKMNE
Position: 241-260
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(251-270)
Chemical type: Peptide from protein
Sequence: GNFGDDKMNEEGIKDGRVTA
Position: 251-270
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(261-280)
Chemical type: Peptide from protein
Sequence: EGIKDGRVTAMLNLTPSPHA
Position: 261-280
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Positive
Host:  2 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(261-280)
Chemical type: Peptide from protein
Sequence: EGIKDGRVTAMLNLTPSPHA
Position: 261-280
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Positive
Host:  2 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(261-280)
Chemical type: Peptide from protein
Sequence: EGIKDGRVTAMLNLTPSPHA
Position: 261-280
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Positive
Host:  2 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(261-280)
Chemical type: Peptide from protein
Sequence: EGIKDGRVTAMLNLTPSPHA
Position: 261-280
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Positive
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(261-280)
Chemical type: Peptide from protein
Sequence: EGIKDGRVTAMLNLTPSPHA
Position: 261-280
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Positive
Host:  2 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(261-280)
Chemical type: Peptide from protein
Sequence: EGIKDGRVTAMLNLTPSPHA
Position: 261-280
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(271-290)
Chemical type: Peptide from protein
Sequence: MLNLTPSPHACLFGSRVTPK
Position: 271-290
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(271-290)
Chemical type: Peptide from protein
Sequence: MLNLTPSPHACLFGSRVTPK
Position: 271-290
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Positive
Host:  2 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(271-290)
Chemical type: Peptide from protein
Sequence: MLNLTPSPHACLFGSRVTPK
Position: 271-290
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Positive
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(271-290)
Chemical type: Peptide from protein
Sequence: MLNLTPSPHACLFGSRVTPK
Position: 271-290
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Positive
Host:  2 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(271-290)
Chemical type: Peptide from protein
Sequence: MLNLTPSPHACLFGSRVTPK
Position: 271-290
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(271-290)
Chemical type: Peptide from protein
Sequence: MLNLTPSPHACLFGSRVTPK
Position: 271-290
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(281-300)
Chemical type: Peptide from protein
Sequence: CLFGSRVTPKLQPDGLHLKF
Position: 281-300
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(291-310)
Chemical type: Peptide from protein
Sequence: LQPDGLHLKFEFTTVVPRDD
Position: 291-310
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(301-320)
Chemical type: Peptide from protein
Sequence: EFTTVVPRDDPQFDNYVKIC
Position: 301-320
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(311-330)
Chemical type: Peptide from protein
Sequence: PQFDNYVKICDECVDGVGTR
Position: 311-330
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(321-340)
Chemical type: Peptide from protein
Sequence: DECVDGVGTRPKDEVVRPKS
Position: 321-340
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(331-350)
Chemical type: Peptide from protein
Sequence: PKDEVVRPKSRSSSRPATRG
Position: 331-350
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(341-360)
Chemical type: Peptide from protein
Sequence: RSSSRPATRGNSPAPKQQRQ
Position: 341-360
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(361-380)
Chemical type: Peptide from protein
Sequence: KKEKKPKKQEDEVDKALTSD
Position: 361-380
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(371-390)
Chemical type: Peptide from protein
Sequence: DEVDKALTSDEERNNAQLEF
Position: 371-390
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(381-400)
Chemical type: Peptide from protein
Sequence: EERNNAQLEFDDEPKVINWG
Position: 381-400
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Positive
Host:  2 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(381-400)
Chemical type: Peptide from protein
Sequence: EERNNAQLEFDDEPKVINWG
Position: 381-400
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(381-400)
Chemical type: Peptide from protein
Sequence: EERNNAQLEFDDEPKVINWG
Position: 381-400
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Positive
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(381-400)
Chemical type: Peptide from protein
Sequence: EERNNAQLEFDDEPKVINWG
Position: 381-400
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(381-400)
Chemical type: Peptide from protein
Sequence: EERNNAQLEFDDEPKVINWG
Position: 381-400
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Positive
Host:  2 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(381-400)
Chemical type: Peptide from protein
Sequence: EERNNAQLEFDDEPKVINWG
Position: 381-400
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N(391-410)
Chemical type: Peptide from protein
Sequence: DDEPKVINWGDSALGENEL
Position: 391-409
Source molecule (GenBank): ATJ01110.1
Assay category: TCell
Result: Negative
Host:  2 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo