Reference: Leslie P Cousens; Yan Su; Elizabeth McClaine; Xin Li; Frances Terry; Robert Smith; Jinhee Lee; William Martin; David W Scott; Anne S De Groot. "Application of IgG-derived natural Treg epitopes (IgG Tregitopes) to antigen-specific tolerance induction in a murine model of type 1 diabetes." Journal of diabetes research (2013).

Epitope: GAD65 (86-103)
Chemical type: Peptide from protein
Sequence: DVNYAFLHATDLLPACDG
Position: 86-103
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (86-103)
Chemical type: Peptide from protein
Sequence: DVNYAFLHATDLLPACDG
Position: 86-103
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (86-103)
Chemical type: Peptide from protein
Sequence: DVNYAFLHATDLLPACDG
Position: 86-103
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 5000.0

Epitope: GAD65 (86-103)
Chemical type: Peptide from protein
Sequence: DVNYAFLHATDLLPACDG
Position: 86-103
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 7500.0

Epitope: GAD65 (86-103)
Chemical type: Peptide from protein
Sequence: DVNYAFLHATDLLPACDG
Position: 86-103
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 5000.0

Epitope: GAD65 (86-103)
Chemical type: Peptide from protein
Sequence: DVNYAFLHATDLLPACDG
Position: 86-103
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD65 (175-190)
Chemical type: Peptide from protein
Sequence: HPRYFNQLSTGLDMVG
Position: 175-190
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (175-190)
Chemical type: Peptide from protein
Sequence: HPRYFNQLSTGLDMVG
Position: 175-190
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (175-190)
Chemical type: Peptide from protein
Sequence: HPRYFNQLSTGLDMVG
Position: 175-190
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 10700.0

Epitope: GAD65 (175-190)
Chemical type: Peptide from protein
Sequence: HPRYFNQLSTGLDMVG
Position: 175-190
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 50000.0

Epitope: GAD65 (175-190)
Chemical type: Peptide from protein
Sequence: HPRYFNQLSTGLDMVG
Position: 175-190
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 13900.0

Epitope: GAD65 (175-190)
Chemical type: Peptide from protein
Sequence: HPRYFNQLSTGLDMVG
Position: 175-190
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD65 (203-226)
Chemical type: Peptide from protein
Sequence: NMFTYEIAPVFVLLEYVTLKKMRE
Position: 203-226
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (203-226)
Chemical type: Peptide from protein
Sequence: NMFTYEIAPVFVLLEYVTLKKMRE
Position: 203-226
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (203-226)
Chemical type: Peptide from protein
Sequence: NMFTYEIAPVFVLLEYVTLKKMRE
Position: 203-226
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 25000.0

Epitope: GAD65 (243-267)
Chemical type: Peptide from protein
Sequence: GAISNMYAMMIARFKMFPEVKEKGM
Position: 243-267
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (243-267)
Chemical type: Peptide from protein
Sequence: GAISNMYAMMIARFKMFPEVKEKGM
Position: 243-267
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (243-267)
Chemical type: Peptide from protein
Sequence: GAISNMYAMMIARFKMFPEVKEKGM
Position: 243-267
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD65 (243-267)
Chemical type: Peptide from protein
Sequence: GAISNMYAMMIARFKMFPEVKEKGM
Position: 243-267
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 5000.0

Epitope: GAD65 (243-267)
Chemical type: Peptide from protein
Sequence: GAISNMYAMMIARFKMFPEVKEKGM
Position: 243-267
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 8700.0

Epitope: GAD65 (243-267)
Chemical type: Peptide from protein
Sequence: GAISNMYAMMIARFKMFPEVKEKGM
Position: 243-267
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 5000.0

Epitope: GAD65 (264-287)
Chemical type: Peptide from protein
Sequence: EKGMAALPRLIAFTSEHSHFSLKK
Position: 264-287
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (264-287)
Chemical type: Peptide from protein
Sequence: EKGMAALPRLIAFTSEHSHFSLKK
Position: 264-287
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (264-287)
Chemical type: Peptide from protein
Sequence: EKGMAALPRLIAFTSEHSHFSLKK
Position: 264-287
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 25000.0

Epitope: GAD65 (338-352)
Chemical type: Peptide from protein
Sequence: TTVYGAFDPLLAVAD
Position: 338-352
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (338-352)
Chemical type: Peptide from protein
Sequence: TTVYGAFDPLLAVAD
Position: 338-352
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (338-352)
Chemical type: Peptide from protein
Sequence: TTVYGAFDPLLAVAD
Position: 338-352
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 4100.0

Epitope: GAD65 (338-352)
Chemical type: Peptide from protein
Sequence: TTVYGAFDPLLAVAD
Position: 338-352
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD65 (338-352)
Chemical type: Peptide from protein
Sequence: TTVYGAFDPLLAVAD
Position: 338-352
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD65 (338-352)
Chemical type: Peptide from protein
Sequence: TTVYGAFDPLLAVAD
Position: 338-352
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 31000.0

Epitope: GAD65 (354-372)
Chemical type: Peptide from protein
Sequence: CKKYKIWMHVDAAWGGGLL
Position: 354-372
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (354-372)
Chemical type: Peptide from protein
Sequence: CKKYKIWMHVDAAWGGGLL
Position: 354-372
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (354-372)
Chemical type: Peptide from protein
Sequence: CKKYKIWMHVDAAWGGGLL
Position: 354-372
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD65 (354-372)
Chemical type: Peptide from protein
Sequence: CKKYKIWMHVDAAWGGGLL
Position: 354-372
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 6400.0

Epitope: GAD65 (354-372)
Chemical type: Peptide from protein
Sequence: CKKYKIWMHVDAAWGGGLL
Position: 354-372
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 5000.0

Epitope: GAD65 (354-372)
Chemical type: Peptide from protein
Sequence: CKKYKIWMHVDAAWGGGLL
Position: 354-372
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 50000.0

Epitope: GAD65 (369-392)
Chemical type: Peptide from protein
Sequence: GGLLMSRKHKWKLSGVERANSVTW
Position: 369-392
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (369-392)
Chemical type: Peptide from protein
Sequence: GGLLMSRKHKWKLSGVERANSVTW
Position: 369-392
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (369-392)
Chemical type: Peptide from protein
Sequence: GGLLMSRKHKWKLSGVERANSVTW
Position: 369-392
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 16300.0

Epitope: GAD65 (369-392)
Chemical type: Peptide from protein
Sequence: GGLLMSRKHKWKLSGVERANSVTW
Position: 369-392
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 5000.0

Epitope: GAD65 (369-392)
Chemical type: Peptide from protein
Sequence: GGLLMSRKHKWKLSGVERANSVTW
Position: 369-392
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 5000.0

Epitope: GAD65 (369-392)
Chemical type: Peptide from protein
Sequence: GGLLMSRKHKWKLSGVERANSVTW
Position: 369-392
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD65 (405-424)
Chemical type: Peptide from protein
Sequence: SALLVREEGLMQNCNQMHAS
Position: 405-424
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (405-424)
Chemical type: Peptide from protein
Sequence: SALLVREEGLMQNCNQMHAS
Position: 405-424
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (405-424)
Chemical type: Peptide from protein
Sequence: SALLVREEGLMQNCNQMHAS
Position: 405-424
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD65 (405-424)
Chemical type: Peptide from protein
Sequence: SALLVREEGLMQNCNQMHAS
Position: 405-424
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD65 (405-424)
Chemical type: Peptide from protein
Sequence: SALLVREEGLMQNCNQMHAS
Position: 405-424
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 50000.0

Epitope: GAD65 (405-424)
Chemical type: Peptide from protein
Sequence: SALLVREEGLMQNCNQMHAS
Position: 405-424
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD65 (450-470)
Chemical type: Peptide from protein
Sequence: VDVFKLWLMWRAKGTTGFEAH
Position: 450-470
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (450-470)
Chemical type: Peptide from protein
Sequence: VDVFKLWLMWRAKGTTGFEAH
Position: 450-470
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (450-470)
Chemical type: Peptide from protein
Sequence: VDVFKLWLMWRAKGTTGFEAH
Position: 450-470
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 25400.0

Epitope: GAD65 (450-470)
Chemical type: Peptide from protein
Sequence: VDVFKLWLMWRAKGTTGFEAH
Position: 450-470
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD65 (450-470)
Chemical type: Peptide from protein
Sequence: VDVFKLWLMWRAKGTTGFEAH
Position: 450-470
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 10200.0

Epitope: GAD65 (450-470)
Chemical type: Peptide from protein
Sequence: VDVFKLWLMWRAKGTTGFEAH
Position: 450-470
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 11400.0

Epitope: GAD65 (480-496)
Chemical type: Peptide from protein
Sequence: YLYNIIKNREGYEMVFD
Position: 480-496
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (480-496)
Chemical type: Peptide from protein
Sequence: YLYNIIKNREGYEMVFD
Position: 480-496
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (480-496)
Chemical type: Peptide from protein
Sequence: YLYNIIKNREGYEMVFD
Position: 480-496
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 5000.0

Epitope: GAD65 (480-496)
Chemical type: Peptide from protein
Sequence: YLYNIIKNREGYEMVFD
Position: 480-496
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 5700.0

Epitope: GAD65 (480-496)
Chemical type: Peptide from protein
Sequence: YLYNIIKNREGYEMVFD
Position: 480-496
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD65 (480-496)
Chemical type: Peptide from protein
Sequence: YLYNIIKNREGYEMVFD
Position: 480-496
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 5000.0

Epitope: GAD65 (504-518)
Chemical type: Peptide from protein
Sequence: VCFWYIPPSLRTLED
Position: 504-518
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (504-518)
Chemical type: Peptide from protein
Sequence: VCFWYIPPSLRTLED
Position: 504-518
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (504-518)
Chemical type: Peptide from protein
Sequence: VCFWYIPPSLRTLED
Position: 504-518
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 95800.0

Epitope: GAD65 (504-518)
Chemical type: Peptide from protein
Sequence: VCFWYIPPSLRTLED
Position: 504-518
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD65 (504-518)
Chemical type: Peptide from protein
Sequence: VCFWYIPPSLRTLED
Position: 504-518
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 25200.0

Epitope: GAD65 (504-518)
Chemical type: Peptide from protein
Sequence: VCFWYIPPSLRTLED
Position: 504-518
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 10300.0

Epitope: GAD65 (511-525)
Chemical type: Peptide from protein
Sequence: PSLRTLEDNEERMSR
Position: 511-525
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (511-525)
Chemical type: Peptide from protein
Sequence: PSLRTLEDNEERMSR
Position: 511-525
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (511-525)
Chemical type: Peptide from protein
Sequence: PSLRTLEDNEERMSR
Position: 511-525
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD65 (511-525)
Chemical type: Peptide from protein
Sequence: PSLRTLEDNEERMSR
Position: 511-525
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD65 (511-525)
Chemical type: Peptide from protein
Sequence: PSLRTLEDNEERMSR
Position: 511-525
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD65 (511-525)
Chemical type: Peptide from protein
Sequence: PSLRTLEDNEERMSR
Position: 511-525
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD65 (550-570)
Chemical type: Peptide from protein
Sequence: LGDKVNFFRMVISNPAATHQD
Position: 550-570
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (550-570)
Chemical type: Peptide from protein
Sequence: LGDKVNFFRMVISNPAATHQD
Position: 550-570
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 (550-570)
Chemical type: Peptide from protein
Sequence: LGDKVNFFRMVISNPAATHQD
Position: 550-570
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 25000.0