Reference: Christian F Teh-Poot; Andrea Alfaro-Chacón; Landy M Pech-Pisté; Miguel E Rosado-Vallado; Oluwatoyin Ajibola Asojo; Liliana E Villanueva-Lizama; Eric Dumonteil; Julio Vladimir Cruz-Chan. "Immunogenicity of  Multi-Epitope Recombinant Protein as an Antigen Candidate for Chagas Disease Vaccine in Humans." Pathogens (Basel, Switzerland) (2025).

Epitope: Tc1
Chemical type: Peptide from protein
Sequence: ALLQFLLAV
Position: 132-140
Source molecule (GenBank): 1393612918
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc2
Chemical type: Peptide from protein
Sequence: ALNESLSSL
Position: 49-57
Source molecule (GenBank): 1393602452
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc3
Chemical type: Peptide from protein
Sequence: ALWSGFTSI
Position: 15-23
Source molecule (GenBank): 28475411
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc4
Chemical type: Peptide from protein
Sequence: FILDAPVAV
Position: 14-22
Source molecule (GenBank): 1243003472
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc5
Chemical type: Peptide from protein
Sequence: FLAAAPLFV
Position: 750-758
Source molecule (GenBank): 1511107254
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc6
Chemical type: Peptide from protein
Sequence: FLAGYNATV
Position: 66-74
Source molecule (GenBank): 407859374
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc7
Chemical type: Peptide from protein
Sequence: FLLHLSLNV
Position: 858-866
Source molecule (GenBank): 1393613979
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc8
Chemical type: Peptide from protein
Sequence: FLSESLEQL
Position: 649-657
Source molecule (GenBank): 1243003472
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc9
Chemical type: Peptide from protein
Sequence: GLMQGMFAL
Position: 107-115
Source molecule (GenBank): 557864725
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc10
Chemical type: Peptide from protein
Sequence: ILASVFFGV
Position: 311-319
Source molecule (GenBank): 407847897
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc11
Chemical type: Peptide from protein
Sequence: ILCDFLLHV
Position: 226-234
Source molecule (GenBank): 1393601807
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc12
Chemical type: Peptide from protein
Sequence: ILLEAIYKL
Position: 140-148
Source molecule (GenBank): 1393615922
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc13
Chemical type: Peptide from protein
Sequence: ILVSFYITV
Position: 250-258
Source molecule (GenBank): 407847684
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc14
Chemical type: Peptide from protein
Sequence: KLDGVLTQV
Position: 321-329
Source molecule (GenBank): 1393615350
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc15
Chemical type: Peptide from protein
Sequence: KLFDFFWRI
Position: 141-149
Source molecule (GenBank): 71668524
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc16
Chemical type: Peptide from protein
Sequence: KLLQNMFEV
Position: 352-360
Source molecule (GenBank): 407844808
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc17
Chemical type: Peptide from protein
Sequence: KLWAFLWSI
Position: 293-301
Source molecule (GenBank): 1393608547
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc18
Chemical type: Peptide from protein
Sequence: LLMDCAAYL
Position: 166-174
Source molecule (GenBank): 71406618
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc19
Chemical type: Peptide from protein
Sequence: LLMDDFSAV
Position: 113-121
Source molecule (GenBank): 1509454191
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc21
Chemical type: Peptide from protein
Sequence: MLLLALAYI
Position: 111-119
Source molecule (GenBank): 1393615932
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc22
Chemical type: Peptide from protein
Sequence: MLMEAAVEL
Position: 218-226
Source molecule (GenBank): 1393603109
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc23
Chemical type: Peptide from protein
Sequence: RLLEEIINL
Position: 31-39
Source molecule (GenBank): 1393615521
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc24
Chemical type: Peptide from protein
Sequence: RLMMSYFEV
Position: 401-409
Source molecule (GenBank): 1393614155
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc25
Chemical type: Peptide from protein
Sequence: SMIAPLWSV
Position: 888-896
Source molecule (GenBank): 407852018
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc26
Chemical type: Peptide from protein
Sequence: TLMSFSIFV
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc26
Chemical type: Peptide from protein
Sequence: TLMSFSIFV
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc27
Chemical type: Peptide from protein
Sequence: VLFTTSAHV
Position: 108-116
Source molecule (GenBank): 1393614572
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc29
Chemical type: Peptide from protein
Sequence: VMMPLIFLI
Position: 245-253
Source molecule (GenBank): 1393608553
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc30
Chemical type: Peptide from protein
Sequence: YLADVFVIL
Position: 438-446
Source molecule (GenBank): 1393608558
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc31
Chemical type: Peptide from protein
Sequence: YLDDALDVV
Position: 40-48
Source molecule (GenBank): 407849932
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc32
Chemical type: Peptide from protein
Sequence: YLIPISLFV
Position: 350-358
Source molecule (GenBank): 1393607799
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc33
Chemical type: Peptide from protein
Sequence: YLLEVIVQV
Position: 286-294
Source molecule (GenBank): 407844808
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc34
Chemical type: Peptide from protein
Sequence: YLLPLLHTV
Position: 1199-1207
Source molecule (GenBank): 1393588465
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc35
Chemical type: Peptide from protein
Sequence: YMLSRVAAV
Position: 51-59
Source molecule (GenBank): 1393616443
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc36
Chemical type: Peptide from protein
Sequence: QLLEHLVEL
Position: 240-248
Source molecule (GenBank): 1393615668
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc37
Chemical type: Peptide from protein
Sequence: FLYNRPLSV
Position: 640-648
Source molecule (GenBank): 473768
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc38
Chemical type: Peptide from protein
Sequence: VLLPSLFLL
Position: 921-929
Source molecule (GenBank): 71652553
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc39
Chemical type: Peptide from protein
Sequence: KLDEFTSRV
Position: 79-87
Source molecule (GenBank): 1393587138
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Tc40
Chemical type: Peptide from protein
Sequence: TLLYATVEV
Position: 381-389
Source molecule (GenBank): 1393611861
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo