Reference: Jan Kisielow; Franz-Josef Obermair; Manfred Kopf. "Deciphering CD4<sup>+</sup> T cell specificity using novel MHC-TCR chimeric receptors." Nature immunology (2019).

Epitope: NP264
Chemical type: Peptide from protein
Sequence: LILRGSVAHKSCL
Position: 264-276
Source molecule (GenBank): P03466.2
Assay category: TCell
Result: Positive-High
Cell type: T cell CD4+
Tissue: lung
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: NP4
Chemical type: Peptide from protein
Sequence: QNSQVYSLIRPNENPAHK
Position: 308-325
Source molecule (GenBank): P03466.2
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: lung
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: NS1 3
Chemical type: Peptide from protein
Sequence: LIPKQKVAGPLCIRM
Position: 105-119
Source molecule (GenBank): P03496.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: lung
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: HA5
Chemical type: Peptide from protein
Sequence: PVRSWSYIVETPNSENGICYPGDFID
Position: 89-114
Source molecule (GenBank): P03452
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: lung
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: G1
Chemical type: Peptide, no natural source
Sequence: VHYRGSSPHKAL
Assay category: TCell
Result: Positive-High
Cell type: T cell CD4+
Tissue: lung
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: E3
Chemical type: Peptide, no natural source
Sequence: RHYRGSIALKAT
Assay category: TCell
Result: Positive-Intermediate
Cell type: T cell CD4+
Tissue: lung
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: D1
Chemical type: Peptide, no natural source
Sequence: LSFATPYAHKSI
Assay category: TCell
Result: Positive-Intermediate
Cell type: T cell CD4+
Tissue: lung
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: D1
Chemical type: Peptide, no natural source
Sequence: LSFATPYAHKSI
Assay category: TCell
Result: Positive-Intermediate
Cell type: T cell CD4+
Tissue: lung
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: E1
Chemical type: Peptide, no natural source
Sequence: YGFLVPNPTKAT
Assay category: TCell
Result: Positive-Intermediate
Cell type: T cell CD4+
Tissue: lung
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: E7
Chemical type: Peptide, no natural source
Sequence: TIYRLATAMKAT
Assay category: TCell
Result: Positive-Intermediate
Cell type: T cell CD4+
Tissue: lung
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: D5
Chemical type: Peptide, no natural source
Sequence: KCFVGSVPHKSL
Assay category: TCell
Result: Positive-Intermediate
Cell type: T cell CD4+
Tissue: lung
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: C6
Chemical type: Peptide, no natural source
Sequence: SQFFYPPPHKAP
Assay category: TCell
Result: Positive-Intermediate
Cell type: T cell CD4+
Tissue: lung
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: E2
Chemical type: Peptide, no natural source
Sequence: MVFHVSYAHKSL
Assay category: TCell
Result: Positive-Intermediate
Cell type: T cell CD4+
Tissue: lung
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: C7
Chemical type: Peptide, no natural source
Sequence: EKFTTPPAHKSF
Assay category: TCell
Result: Positive-Intermediate
Cell type: T cell CD4+
Tissue: lung
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: D6
Chemical type: Peptide, no natural source
Sequence: CHFIYSSAHKSY
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: lung
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: D2
Chemical type: Peptide, no natural source
Sequence: YCFFTSVPHKSC
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: lung
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: A8
Chemical type: Peptide, no natural source
Sequence: FLYRAALPVKSY
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: lung
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: NP311
Chemical type: Peptide from protein
Sequence: EGWPYIACRTSVVGR
Position: 311-325
Source molecule (GenBank): P07400.1
Assay category: TCell
Result: Positive-High
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: NP547
Chemical type: Peptide from protein
Sequence: DLIFRGPNVV
Position: 547-556
Source molecule (GenBank): P07400.1
Assay category: TCell
Result: Positive-High
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: gp61-77
Chemical type: Peptide from protein
Sequence: GLNGPDIYKGVYQFKSV
Position: 61-77
Source molecule (GenBank): P07399.1
Assay category: TCell
Result: Positive-High
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: gp61-77
Chemical type: Peptide from protein
Sequence: GLNGPDIYKGVYQFKSV
Position: 61-77
Source molecule (GenBank): P07399.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: gp61-77
Chemical type: Peptide from protein
Sequence: GLNGPDIYKGVYQFKSV
Position: 61-77
Source molecule (GenBank): P07399.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: gp61-77
Chemical type: Peptide from protein
Sequence: GLNGPDIYKGVYQFKSV
Position: 61-77
Source molecule (GenBank): P07399.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: gp61-77
Chemical type: Peptide from protein
Sequence: GLNGPDIYKGVYQFKSV
Position: 61-77
Source molecule (GenBank): P07399.1
Assay category: TCell
Result: Positive-High
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: gp61-77
Chemical type: Peptide from protein
Sequence: GLNGPDIYKGVYQFKSV
Position: 61-77
Source molecule (GenBank): P07399.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: gp61N
Chemical type: Peptide, no natural source
Sequence: GLNGPDIYNGVYQFKSV
Assay category: TCell
Result: Positive-High
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: gp61N
Chemical type: Peptide, no natural source
Sequence: GLNGPDIYNGVYQFKSV
Assay category: TCell
Result: Positive-High
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: gp61N
Chemical type: Peptide, no natural source
Sequence: GLNGPDIYNGVYQFKSV
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: gp61S
Chemical type: Peptide, no natural source
Sequence: GLNGPDIYSGVYQFKSV
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: gp61S
Chemical type: Peptide, no natural source
Sequence: GLNGPDIYSGVYQFKSV
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: gp61S
Chemical type: Peptide, no natural source
Sequence: GLNGPDIYSGVYQFKSV
Assay category: TCell
Result: Positive-High
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: OVA323-339
Chemical type: Peptide from protein
Sequence: ISQAVHAAHAEINEAGR
Position: 324-340
Source molecule (GenBank): P01012.2
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: MP1(107-116)
Chemical type: Peptide from protein
Sequence: ITFHGAKEIS
Position: 107-116
Source molecule (GenBank): P03485.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: EnvRV
Chemical type: Peptide, no natural source
Sequence: CCSGSRVSTPGCSR
Assay category: TCell
Result: Positive
Host: F 
Cell type: T cell CD4+
Tissue: other
Culture conditions: Cell Line / Clone (Hybridoma)

Epitope: EnvRV
Chemical type: Peptide, no natural source
Sequence: CCSGSRVSTPGCSR
Assay category: TCell
Result: Positive-High
Cell type: T cell CD4+
Tissue: other
Culture conditions: In Vivo

Epitope: EnvRV
Chemical type: Peptide, no natural source
Sequence: CCSGSRVSTPGCSR
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo