Reference: Jennifer L Nayak; Katherine A Richards; Francisco A Chaves; Andrea J Sant. "Analyses of the specificity of CD4 T cells during the primary immune response to influenza virus reveals dramatic MHC-linked asymmetries in reactivity to individual viral proteins." Viral immunology (2010).

Epitope: HA 16<sup>90-106</sup>
Chemical type: Peptide from protein
Sequence: KESWSYIVETPNPENGT
Position: 90-106
Source molecule (GenBank): ABF21272.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 35<sup>203-219</sup>
Chemical type: Peptide from protein
Sequence: NQRALYHTENAYVSVVS
Position: 203-219
Source molecule (GenBank): ABF21272.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 52<sup>304-320</sup>
Chemical type: Peptide from protein
Sequence: SSLPFQNVHPVTIGECP
Position: 304-320
Source molecule (GenBank): ABF21272.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 53<sup>310-326</sup>
Chemical type: Peptide from protein
Sequence: NVHPVTIGECPKYVRSA
Position: 310-326
Source molecule (GenBank): ABF21272.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 53<sup>310-326</sup>
Chemical type: Peptide from protein
Sequence: NVHPVTIGECPKYVRSA
Position: 310-326
Source molecule (GenBank): ABF21272.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NA 19<sup>108-123</sup>
Chemical type: Peptide from protein
Sequence: IGSKGDVFVIREPFIS
Position: 108-123
Source molecule (GenBank): ABQ10080.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NA 30<sup>171-187</sup>
Chemical type: Peptide from protein
Sequence: NSKFESVAWSASACHDG
Position: 171-187
Source molecule (GenBank): ABQ10080.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NA 31<sup>177-193</sup>
Chemical type: Peptide from protein
Sequence: VAWSASACHDGMGWLTI
Position: 177-193
Source molecule (GenBank): ABQ10080.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NA 56<sup>327-343</sup>
Chemical type: Peptide from protein
Sequence: RPKDGEGSCNPVTVDGA
Position: 327-343
Source molecule (GenBank): ABQ10080.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NA 76<sup>446-462</sup>
Chemical type: Peptide from protein
Sequence: FCGVNSDTANWSWPDGA
Position: 446-462
Source molecule (GenBank): ABQ10080.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NA 77<sup>452-468</sup>
Chemical type: Peptide from protein
Sequence: DTANWSWPDGAELPFTI
Position: 452-468
Source molecule (GenBank): ABQ10080.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 25<sup>145-161</sup>
Chemical type: Peptide from protein
Sequence: DTTYQRTRALVRTGMDP
Position: 145-161
Source molecule (GenBank): ABA12733.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 44<sup>258-274</sup>
Chemical type: Peptide from protein
Sequence: FLARSALILRGSVAHKS
Position: 258-274
Source molecule (GenBank): ABA12733.1
Assay category: TCell
Result: Positive-High
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 46<sup>270-286</sup>
Chemical type: Peptide from protein
Sequence: VAHKSCLPACVYGPAVA
Position: 270-286
Source molecule (GenBank): ABA12733.1
Assay category: TCell
Result: Positive-High
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 46<sup>270-286</sup>
Chemical type: Peptide from protein
Sequence: VAHKSCLPACVYGPAVA
Position: 270-286
Source molecule (GenBank): ABA12733.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 48<sup>282-298</sup>
Chemical type: Peptide from protein
Sequence: GPAVASGYDFEKEGYSL
Position: 282-298
Source molecule (GenBank): ABA12733.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 52<sup>306-328</sup>
Chemical type: Peptide from protein
Sequence: LLQTSQVYSLIRPNENP
Position: 306-322
Source molecule (GenBank): ABA12733.1
Assay category: TCell
Result: Positive-High
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 53<sup>312-328</sup>
Chemical type: Peptide from protein
Sequence: VYSLIRPNENPAHKSQL
Position: 312-328
Source molecule (GenBank): ABA12733.1
Assay category: TCell
Result: Positive-High
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 71<sup>420-436</sup>
Chemical type: Peptide from protein
Sequence: FDKTTIMAAFTGNTEGR
Position: 420-436
Source molecule (GenBank): ABA12733.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M1 2<sup>7-23</sup>
Chemical type: Peptide from protein
Sequence: VETYVLSIVPSGPLKAE
Position: 7-23
Source molecule (GenBank): ABA12730.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M1 3<sup>13-29</sup>
Chemical type: Peptide from protein
Sequence: SIVPSGPLKAEIAQRLE
Position: 13-29
Source molecule (GenBank): ABA12730.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M1 24<sup>139-155</sup>
Chemical type: Peptide from protein
Sequence: TTESAFGLICATCEQIA
Position: 139-155
Source molecule (GenBank): ABA12730.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS1 13<sup>72-88</sup>
Chemical type: Peptide from protein
Sequence: ESDEAFKMTMASALASR
Position: 72-88
Source molecule (GenBank): ABA42580.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS1 14<sup>78-94</sup>
Chemical type: Peptide from protein
Sequence: KMTMASALASRYLTDMT
Position: 78-94
Source molecule (GenBank): ABA42580.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS1 19<sup>108-124</sup>
Chemical type: Peptide from protein
Sequence: KQKVAGPLCVRMDQAIM
Position: 108-124
Source molecule (GenBank): ABA42580.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS1 31<sup>180-196</sup>
Chemical type: Peptide from protein
Sequence: VLIGGLEWNDNTVRVSE
Position: 180-196
Source molecule (GenBank): ABA42580.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 22<sup>126-142</sup>
Chemical type: Peptide from protein
Sequence: SSFERFEIFPKESSWPN
Position: 126-142
Source molecule (GenBank): ABF21272.1
Assay category: TCell
Result: Positive-High
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 23<sup>132-148</sup>
Chemical type: Peptide from protein
Sequence: EIFPKESSWPNHTVTGV
Position: 132-148
Source molecule (GenBank): ABF21272.1
Assay category: TCell
Result: Positive-High
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 24<sup>138-154</sup>
Chemical type: Peptide from protein
Sequence: SSWPNHTVTGVSASCSH
Position: 138-154
Source molecule (GenBank): ABF21272.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 25<sup>144-160</sup>
Chemical type: Peptide from protein
Sequence: TVTGVSASCSHNGKSSF
Position: 144-160
Source molecule (GenBank): ABF21272.1
Assay category: TCell
Result: Positive-High
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 38<sup>221-237</sup>
Chemical type: Peptide from protein
Sequence: HYSRRFTPEIAKRPKVR
Position: 221-237
Source molecule (GenBank): ABF21272.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 54<sup>316-332</sup>
Chemical type: Peptide from protein
Sequence: IGECPKYVRSAKLRMVT
Position: 316-332
Source molecule (GenBank): ABF21272.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 55<sup>322-338</sup>
Chemical type: Peptide from protein
Sequence: YVRSAKLRMVTGLRNIP
Position: 322-338
Source molecule (GenBank): ABF21272.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 57<sup>334-350</sup>
Chemical type: Peptide from protein
Sequence: LRNIPSIQSRGLFGAIA
Position: 334-350
Source molecule (GenBank): ABF21272.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 65<sup>380-396</sup>
Chemical type: Peptide from protein
Sequence: DQKSTQNAINGITNKVN
Position: 380-396
Source molecule (GenBank): ABF21272.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NA 45<sup>261-277</sup>
Chemical type: Peptide from protein
Sequence: GKVTKSIELNAPNFHYE
Position: 261-277
Source molecule (GenBank): ABQ10080.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NA 49<sup>285-301</sup>
Chemical type: Peptide from protein
Sequence: TGTVMCVCRDNWHGSNR
Position: 285-301
Source molecule (GenBank): ABQ10080.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NA 61<sup>357-373</sup>
Chemical type: Peptide from protein
Sequence: VWIGRTKSNRLRKGFEM
Position: 357-373
Source molecule (GenBank): ABQ10080.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 4<sup>19-35</sup>
Chemical type: Peptide from protein
Sequence: RQNATEIRASVGRMIGG
Position: 19-35
Source molecule (GenBank): ABA12733.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 16<sup>91-107</sup>
Chemical type: Peptide from protein
Sequence: KTGGPIYKRVDGKWVRE
Position: 91-107
Source molecule (GenBank): ABA12733.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 17<sup>97-113</sup>
Chemical type: Peptide from protein
Sequence: YKRVDGKWVRELVLYDK
Position: 97-113
Source molecule (GenBank): ABA12733.1
Assay category: TCell
Result: Positive-High
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 55<sup>324-340</sup>
Chemical type: Peptide from protein
Sequence: HKSQLVWMACNSAAFED
Position: 324-340
Source molecule (GenBank): ABA12733.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 58<sup>342-358</sup>
Chemical type: Peptide from protein
Sequence: RVSSFIRGTRVLPRGKL
Position: 342-358
Source molecule (GenBank): ABA12733.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 74<sup>438-454</sup>
Chemical type: Peptide from protein
Sequence: SDMRAEIIKMMESARPE
Position: 438-454
Source molecule (GenBank): ABA12733.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 75<sup>444-460</sup>
Chemical type: Peptide from protein
Sequence: IIKMMESARPEEVSFQG
Position: 444-460
Source molecule (GenBank): ABA12733.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M1 31<sup>181-197</sup>
Chemical type: Peptide from protein
Sequence: LASTTAKAMEQMAGSSE
Position: 181-197
Source molecule (GenBank): ABA12730.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M1 36<sup>210-226</sup>
Chemical type: Peptide from protein
Sequence: RQMVQAMRAIGTHPSSS
Position: 210-226
Source molecule (GenBank): ABA12730.1
Assay category: TCell
Result: Positive
Host: F 3 months
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo