Reference: Melinda Y Hardy; Adam Girardin; Catherine Pizzey; Donald J Cameron; Katherine A Watson; Stefania Picascia; Renata Auricchio; Luigi Greco; Carmen Gianfrani; Nicole L La Gruta; Robert P Anderson; Jason A Tye-Din. "Consistency in polyclonal T-cell responses to gluten between children and adults with celiac disease." Gastroenterology (2015).

Epitope: QPFPQPEQPFPWQ
Chemical type: Peptide from protein
Sequence: QPFPQPQQPFPWQ
Position: 105-117
Source molecule (GenBank): AAG17702.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: LQPFPQPELPYPQP
Chemical type: Peptide from protein
Sequence: LQPFPQPQLPYPQP
Position: 78-91
Source molecule (GenBank): P04722.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: LQPFPQPELPFPQP
Chemical type: Peptide from protein
Sequence: LQPFPQPQLPFPQP
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: LQPFPQPELPYSQP
Chemical type: Peptide from protein
Sequence: LQPFPQPQLPYSQP
Position: 59-72
Source molecule (GenBank): CAB76963.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: LPYPQPELPYPQP
Chemical type: Peptide from protein
Sequence: LPYPQPQLPYPQP
Position: 86-98
Source molecule (GenBank): P04724.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: QPFPQPEQPFPWQP
Chemical type: Peptide from protein
Sequence: QPFPQPQQPFPWQP
Position: 105-118
Source molecule (GenBank): AAG17702.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: LQPFPQPELPYPQPQ
Chemical type: Peptide from protein
Sequence: LQPFPQPQLPYPQPQ
Position: 78-92
Source molecule (GenBank): P04722.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: QPFPQPEQPIPVQ
Chemical type: Peptide from protein
Sequence: QPFPQPQQPIPVQ
Position: 182-194
Source molecule (GenBank): AAG17702.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PFPQQPQQPFPQP
Chemical type: Peptide from protein
Sequence: PFPQQPQQPFPQP
Position: 106-118
Source molecule (GenBank): P21292.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PEQPFPEQPEQPF
Chemical type: Peptide from protein
Sequence: PQQPFPQQPQQPF
Position: 80-92
Source molecule (GenBank): P08453.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PEQTFPEQPQLPF
Chemical type: Peptide from protein
Sequence: PQQTFPQQPQLPF
Position: 95-107
Source molecule (GenBank): P21292.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FPQPEQEFPQPQQ
Chemical type: Peptide from protein
Sequence: FPQPQQQFPQPQQ
Position: 135-147
Source molecule (GenBank): P21292.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: YEVIRSLVLRTLPN
Chemical type: Peptide from protein
Sequence: YEVIRSLVLRTLPN
Position: 245-258
Source molecule (GenBank): AAK84776.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PFPLQPEQPFPQP
Chemical type: Peptide from protein
Sequence: PFPLQPQQPFPQP
Position: 77-89
Source molecule (GenBank): AFC98436.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: LPYPQPQLPYPQP
Chemical type: Peptide from protein
Sequence: LPYPQPQLPYPQP
Position: 86-98
Source molecule (GenBank): P04724.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: LQPFPQPQLPYPQP
Chemical type: Peptide from protein
Sequence: LQPFPQPQLPYPQP
Position: 78-91
Source molecule (GenBank): P04722.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: QPFPQPQQPFSQQ
Chemical type: Peptide from protein
Sequence: QPFPQPQQPFSQQ
Position: 36-48
Source molecule (GenBank): AAK84772.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FPQPQQQFPQPQQ
Chemical type: Peptide from protein
Sequence: FPQPQQQFPQPQQ
Position: 135-147
Source molecule (GenBank): P21292.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PFPEQPEQPYPQQ
Chemical type: Peptide from protein
Sequence: PFPQQPQQPYPQQ
Position: 83-95
Source molecule (GenBank): P08079.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PIPQQPQQPFPLQ
Chemical type: Peptide from protein
Sequence: PIPQQPQQPFPLQ
Position: 223-235
Source molecule (GenBank): AAG17702.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: QFIQPEQPFPQQ
Chemical type: Peptide from protein
Sequence: QFIQPQQPFPQQ
Position: 76-87
Source molecule (GenBank): AAK84778.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GQSGYYPTSPQQS
Chemical type: Peptide from protein
Sequence: GQSGYYPTSPQQS
Position: 155-167
Source molecule (GenBank): CAC84119.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: QPFPQPQQPIPVQ
Chemical type: Peptide from protein
Sequence: QPFPQPQQPIPVQ
Position: 182-194
Source molecule (GenBank): AAG17702.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TIPEQPEQPFPLQ
Chemical type: Peptide from protein
Sequence: TIPQQPQQPFPLQ
Position: 199-211
Source molecule (GenBank): AAA92333.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PFPEQPEQPFPQP
Chemical type: Peptide from protein
Sequence: PFPQQPQQPFPQP
Position: 106-118
Source molecule (GenBank): P21292.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GIIQPQQPAQL
Chemical type: Peptide from protein
Sequence: GIIQPQQPAQL
Position: 277-287
Source molecule (GenBank): P08453.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PQQPQQSFPQQQQPA
Chemical type: Peptide from protein
Sequence: PQQPQQSFPQQQQPA
Position: 145-159
Source molecule (GenBank): P21292.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GLERPWQEQPLPPQ
Chemical type: Peptide from protein
Sequence: GLERPWQQQPLPPQ
Position: 5-18
Source molecule (GenBank): BAA22613.2
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: LQPFPQPELPYLQP
Chemical type: Peptide from protein
Sequence: LQPFPQPQLPYLQP
Position: 59-72
Source molecule (GenBank): CAB76962.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: QPFPQPEQPFSQQ
Chemical type: Peptide from protein
Sequence: QPFPQPQQPFSQQ
Position: 36-48
Source molecule (GenBank): AAK84772.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: QPFPQPEQPFCQQ
Chemical type: Peptide from protein
Sequence: QPFPQPQQPFCQQ
Position: 35-47
Source molecule (GenBank): P21292.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: (pE)QQPQQSFPEQERPF
Chemical type: Peptide from protein
Sequence: QQPQQSFPQQQRPF
Position: 159-172
Source molecule (GenBank): P08453.1
Assay category: TCell
Result: Negative
MHC allele types: 314
Host:  3-18 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: (pE)QQSEESEQPFQPQP
Chemical type: Peptide from protein
Sequence: QQSQQSQQPFQPQP
Assay category: TCell
Result: Negative
MHC allele types: 314
Host:  3-18 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: (pE)QPPFSEEQEQPLPQ
Chemical type: Peptide from protein
Sequence: QPPFSQQQQQPLPQ
Position: 47-60
Source molecule (GenBank): AAV92009.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: (pE)QPPFSEQQESPSFSQ
Chemical type: Peptide from protein
Sequence: QPPFSQQQQSPSFSQ
Assay category: TCell
Result: Negative
MHC allele types: 314
Host:  3-18 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: (pE)GIIPEQPAQLEGI
Chemical type: Peptide from protein
Sequence: GIIPQQPAQLEGI
Assay category: TCell
Result: Negative
MHC allele types: 314
Host:  3-18 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: (pE)QPFRPEQPYPQPQP
Chemical type: Peptide from protein
Sequence: QPFRPQQPYPQPQP
Position: 92-105
Source molecule (GenBank): P04721.1
Assay category: TCell
Result: Negative
MHC allele types: 314
Host:  3-18 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: QPQQPQQSFPQQQRPF
Chemical type: Peptide from protein
Sequence: QPQQPQQSFPQQQRPF
Position: 157-172
Source molecule (GenBank): P08453.1
Assay category: TCell
Result: Negative
MHC allele types: 314
Host:  3-18 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: QPQPFPQQSEQSQQPFQPQPF
Chemical type: Peptide from protein
Sequence: QPQPFPQQSEQSQQPFQPQPF
Assay category: TCell
Result: Negative
MHC allele types: 314
Host:  3-18 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: QQPPFSQQQQQPLPQ
Chemical type: Peptide from protein
Sequence: QQPPFSQQQQQPLPQ
Position: 46-60
Source molecule (GenBank): AAV92009.1
Assay category: TCell
Result: Negative
MHC allele types: 314
Host:  3-18 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: QQQQPPFQQQQSPFSQQQQ
Chemical type: Peptide from protein
Sequence: QQQQPPFQQQQSPFSQQQQ
Assay category: TCell
Result: Negative
MHC allele types: 314
Host:  3-18 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: PFRPQQPYPQPQPQ
Chemical type: Peptide from protein
Sequence: PFRPQQPYPQPQPQ
Position: 93-106
Source molecule (GenBank): P04721.1
Assay category: TCell
Result: Negative
MHC allele types: 314
Host:  3-18 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: QPFPQPEQPFPLQ
Chemical type: Peptide from protein
Sequence: QPFPQPQQPFPLQ
Position: 164-176
Source molecule (GenBank): AAB28161.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: QPFPQPEQPIPYQ
Chemical type: Peptide from protein
Sequence: QPFPQPQQPIPYQ
Position: 110-122
Source molecule (GenBank): AAB28161.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PEQPFPEQPQPYPQQP
Chemical type: Peptide from protein
Sequence: PQQPFPQQPQPYPQQP
Position: 25-40
Source molecule (GenBank): CAA37729.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PEQPFQPEQPFPQQ
Chemical type: Peptide from protein
Sequence: PQQPFQPQQPFPQQ
Position: 44-57
Source molecule (GenBank): CAA37729.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PEQPQPFPEQPVPQQP
Chemical type: Peptide from protein
Sequence: PQQPQPFPQQPVPQQP
Position: 24-39
Source molecule (GenBank): 1103203B
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: QPFPQPEQPFSWQ
Chemical type: Peptide from protein
Sequence: QPFPQPQQPFSWQ
Position: 137-149
Source molecule (GenBank): AAA92333.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: QPFPQPEQPFRQQ
Chemical type: Peptide from protein
Sequence: QPFPQPQQPFRQQ
Position: 30-42
Source molecule (GenBank): P17991.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: QEFPQPEQPFPQQ
Chemical type: Peptide from protein
Sequence: QQFPQPQQPFPQQ
Position: 50-62
Source molecule (GenBank): AEW46823.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: QPFPQPEQPIPQQ
Chemical type: Peptide from protein
Sequence: QPFPQPQQPIPQQ
Position: 104-116
Source molecule (GenBank): AAB58403
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: QPFPQPEQPTPIQ
Chemical type: Peptide from protein
Sequence: QPFPQPQQPTPIQ
Position: 66-78
Source molecule (GenBank): CAA42836.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: QPFPQPEQQLPLQ
Chemical type: Peptide from protein
Sequence: QPFPQPQQQLPLQ
Position: 89-101
Source molecule (GenBank): CAA42836.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: QPFPQPEQELPLQ
Chemical type: Peptide from protein
Sequence: QPFPQPQQQLPLQ
Position: 89-101
Source molecule (GenBank): CAA42836.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: QLFPLPEQPFPQP
Chemical type: Peptide from protein
Sequence: QLFPLPQQPFPQP
Position: 3-15
Source molecule (GenBank): CAA26449.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: QPFPQPEQPFPQS
Chemical type: Peptide from protein
Sequence: QPFPQPQQPFPQS
Position: 271-283
Source molecule (GenBank): ABO32294.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PFPLQPEQPVPEQPQ
Chemical type: Peptide from protein
Sequence: PFPLQPQQPVPQQPQ
Position: 242-256
Source molecule (GenBank): CAA42836.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: LPFPQPEQPFVVV
Chemical type: Peptide from protein
Sequence: LPFPQPQQPFVVV
Position: 344-356
Source molecule (GenBank): CAA42836.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: QPEQPFPLQPEQPVP
Chemical type: Peptide from protein
Sequence: QPQQPFPLQPQQPVP
Position: 238-252
Source molecule (GenBank): CAA42836.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PQPRPELPYPQP
Chemical type: Peptide from protein
Sequence: PQPRPQLPYPQP
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: QPFPQPQQPFPWQ
Chemical type: Peptide from protein
Sequence: QPFPQPQQPFPWQ
Position: 105-117
Source molecule (GenBank): AAG17702.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: QAFPQPQQTFPHQ
Chemical type: Peptide from protein
Sequence: QAFPQPQQTFPHQ
Position: 66-78
Source molecule (GenBank): AAK84772.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: IQVDPSGEVEWPQQ
Chemical type: Peptide from protein
Sequence: IQVDPSGQVQWPQQ
Position: 21-34
Source molecule (GenBank): AAK84772.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: IQVDPSGEVQWPQQ
Chemical type: Peptide from protein
Sequence: IQVDPSGQVQWPQQ
Position: 21-34
Source molecule (GenBank): AAK84772.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: IQVDPSGQVEWPQQ
Chemical type: Peptide from protein
Sequence: IQVDPSGQVQWPQQ
Position: 21-34
Source molecule (GenBank): AAK84772.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: IQVDPSGQVQWPQQ
Chemical type: Peptide from protein
Sequence: IQVDPSGQVQWPQQ
Position: 21-34
Source molecule (GenBank): AAK84772.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GQPGYYPTSPQQIGQ
Chemical type: Peptide from protein
Sequence: GQPGYYPTSPQQIGQ
Position: 527-541
Source molecule (GenBank): BAN29068.1
Assay category: TCell
Result: Positive
MHC allele types: 314
Host:  3-18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo