Reference: Angela Wahl; William McCoy; Fredda Schafer; Wilfried Bardet; Rico Buchli; Daved H Fremont; William H Hildebrand. "T-cell tolerance for variability in an HLA class I-presented influenza A virus epitope." Journal of virology (2009).

Epitope: Inluenza PR8 NP<sub>418-426</sub>
Chemical type: Peptide from protein
Sequence: LPFDRTTVM
Position: 418-426
Source molecule (GenBank): NP_040982.1
Assay category: TCell
Result: Positive
Host:  6-8 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Inluenza PR8 NP<sub>418-426</sub>
Chemical type: Peptide from protein
Sequence: LPFDRTTVM
Position: 418-426
Source molecule (GenBank): NP_040982.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Inluenza PR8 NP<sub>418-426</sub>
Chemical type: Peptide from protein
Sequence: LPFDRTTVM
Position: 418-426
Source molecule (GenBank): NP_040982.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 70.8

Epitope: Influenza 7485 NP<sub>418-426</sub>
Chemical type: Peptide from protein
Sequence: LPFDKTTIM
Position: 418-426
Source molecule (GenBank): ACX46229.1
Assay category: TCell
Result: Positive
Host:  6-8 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Influenza 7485 NP<sub>418-426</sub>
Chemical type: Peptide from protein
Sequence: LPFDKTTIM
Position: 418-426
Source molecule (GenBank): ACX46229.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Influenza 7485 NP<sub>418-426</sub>
Chemical type: Peptide from protein
Sequence: LPFDKTTIM
Position: 418-426
Source molecule (GenBank): ACX46229.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 192.3

Epitope: Inluenza 309 NP<sub>418-426</sub>
Chemical type: Peptide from protein
Sequence: LPFEKSTIM
Position: 418-426
Source molecule (GenBank): ABA18030.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Inluenza 309 NP<sub>418-426</sub>
Chemical type: Peptide from protein
Sequence: LPFEKSTIM
Position: 418-426
Source molecule (GenBank): ABA18030.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Inluenza 309 NP<sub>418-426</sub>
Chemical type: Peptide from protein
Sequence: LPFEKSTIM
Position: 418-426
Source molecule (GenBank): ABA18030.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Inluenza 309 NP<sub>418-426</sub>
Chemical type: Peptide from protein
Sequence: LPFEKSTIM
Position: 418-426
Source molecule (GenBank): ABA18030.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 7780.4

Epitope: Inluenza NP<sub>418-426</sub> 4
Chemical type: Peptide from protein
Sequence: LPFDRPTIM
Position: 418-426
Source molecule (GenBank): P15682.1
Assay category: TCell
Result: Positive
Host:  6-8 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Inluenza NP<sub>418-426</sub> 4
Chemical type: Peptide from protein
Sequence: LPFDRPTIM
Position: 418-426
Source molecule (GenBank): P15682.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 7906.8

Epitope: Inluenza NP<sub>418-426</sub> 5
Chemical type: Peptide from protein
Sequence: LPFDKATIM
Position: 418-426
Source molecule (GenBank): ABI21215.1
Assay category: TCell
Result: Positive
Host:  6-8 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Inluenza NP<sub>418-426</sub> 5
Chemical type: Peptide from protein
Sequence: LPFDKATIM
Position: 418-426
Source molecule (GenBank): ABI21215.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 4456.6

Epitope: Inluenza NP<sub>418-426</sub> 6
Chemical type: Peptide from protein
Sequence: LPFEKTTIM
Position: 418-426
Source molecule (GenBank): AAF99666.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Inluenza NP<sub>418-426</sub> 6
Chemical type: Peptide from protein
Sequence: LPFEKTTIM
Position: 418-426
Source molecule (GenBank): AAF99666.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1191.2

Epitope: Inluenza NP<sub>418-426</sub> 7
Chemical type: Peptide from protein
Sequence: LPFERSTIM
Position: 418-426
Source molecule (GenBank): ACX46207.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Inluenza NP<sub>418-426</sub> 7
Chemical type: Peptide from protein
Sequence: LPFERSTIM
Position: 418-426
Source molecule (GenBank): ACX46207.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 3235.9

Epitope: Inluenza NP<sub>418-426</sub> 8
Chemical type: Peptide from protein
Sequence: LPFDKITIM
Position: 418-426
Source molecule (GenBank): ABE11694.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Inluenza NP<sub>418-426</sub> 8
Chemical type: Peptide from protein
Sequence: LPFDKITIM
Position: 418-426
Source molecule (GenBank): ABE11694.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 4808.4

Epitope: Inluenza NP<sub>418-426</sub> 9
Chemical type: Peptide from protein
Sequence: LPFERATIM
Position: 418-426
Source molecule (GenBank): ABI36003.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Inluenza NP<sub>418-426</sub> 9
Chemical type: Peptide from protein
Sequence: LPFERATIM
Position: 418-426
Source molecule (GenBank): ABI36003.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 696.6

Epitope: Inluenza NP<sub>418-426</sub> 10
Chemical type: Peptide from protein
Sequence: LPFGKTTIM
Position: 418-426
Source molecule (GenBank): ABB51966.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Inluenza NP<sub>418-426</sub> 10
Chemical type: Peptide from protein
Sequence: LPFGKTTIM
Position: 418-426
Source molecule (GenBank): ABB51966.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 881.0

Epitope: Inluenza NP<sub>418-426</sub> 12
Chemical type: Peptide from protein
Sequence: LPFDKSTIM
Position: 418-426
Source molecule (GenBank): P18072.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Inluenza NP<sub>418-426</sub> 12
Chemical type: Peptide from protein
Sequence: LPFDKSTIM
Position: 418-426
Source molecule (GenBank): P18072.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 3467.4

Epitope: Inluenza NP<sub>418-426</sub> 14
Chemical type: Peptide from protein
Sequence: LPFERATVM
Position: 418-426
Source molecule (GenBank): P26077.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Inluenza NP<sub>418-426</sub> 14
Chemical type: Peptide from protein
Sequence: LPFERATVM
Position: 418-426
Source molecule (GenBank): P26077.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 2162.7

Epitope: Inluenza NP<sub>418-426</sub> 15
Chemical type: Peptide from protein
Sequence: LPFDKPTIM
Position: 418-426
Source molecule (GenBank): P16986.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Inluenza NP<sub>418-426</sub> 15
Chemical type: Peptide from protein
Sequence: LPFDKPTIM
Position: 418-426
Source molecule (GenBank): P16986.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 335.0

Epitope: Inluenza NP<sub>418-426</sub> 16
Chemical type: Peptide from protein
Sequence: LPFERSTVM
Position: 424-432
Source molecule (GenBank): ACF04386.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Inluenza NP<sub>418-426</sub> 16
Chemical type: Peptide from protein
Sequence: LPFERSTVM
Position: 424-432
Source molecule (GenBank): ACF04386.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 149279.4

Epitope: Inluenza NP<sub>418-426</sub> 17
Chemical type: Peptide from protein
Sequence: LPFEKSTVM
Position: 418-426
Source molecule (GenBank): AAK18005.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Inluenza NP<sub>418-426</sub> 17
Chemical type: Peptide from protein
Sequence: LPFEKSTVM
Position: 418-426
Source molecule (GenBank): AAK18005.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1129.8

Epitope: Inluenza NP<sub>418-426</sub> 18
Chemical type: Peptide from protein
Sequence: LPFDKQTIM
Position: 418-426
Source molecule (GenBank): ABB80027.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Inluenza NP<sub>418-426</sub> 18
Chemical type: Peptide from protein
Sequence: LPFDKQTIM
Position: 418-426
Source molecule (GenBank): ABB80027.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 46451.5

Epitope: Inluenza NP<sub>418-426</sub> 19
Chemical type: Peptide from protein
Sequence: LPFEKSIVM
Position: 418-426
Source molecule (GenBank): ABG88425.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Inluenza NP<sub>418-426</sub> 19
Chemical type: Peptide from protein
Sequence: LPFEKSIVM
Position: 418-426
Source molecule (GenBank): ABG88425.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 494.3

Epitope: Inluenza NP<sub>418-426</sub> 20
Chemical type: Peptide from protein
Sequence: LPFERATII
Position: 418-426
Source molecule (GenBank): AAR12361.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: lymphocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Inluenza NP<sub>418-426</sub> 20
Chemical type: Peptide from protein
Sequence: LPFERATII
Position: 418-426
Source molecule (GenBank): AAR12361.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1183.0

Epitope: PR8 NP<sub>418-426</sub> single mutant peptide
Chemical type: Peptide, no natural source
Sequence: LPFERTTVM
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 67.6

Epitope: PR8 NP<sub>418-426</sub> single mutant peptide
Chemical type: Peptide, no natural source
Sequence: LPFGRTTVM
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 39.8

Epitope: PR8 NP<sub>418-426</sub> single mutant peptide
Chemical type: Peptide, no natural source
Sequence: LPFDKTTVM
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 158.5

Epitope: PR8 NP<sub>418-426</sub> single mutant peptide
Chemical type: Peptide, no natural source
Sequence: LPFDRSTVM
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 79.4

Epitope: PR8 NP<sub>418-426</sub> single mutant peptide
Chemical type: Peptide, no natural source
Sequence: LPFDRATVM
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 91.2

Epitope: PR8 NP<sub>418-426</sub> single mutant peptide
Chemical type: Peptide, no natural source
Sequence: LPFDRPTVM
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 91.2

Epitope: PR8 NP<sub>418-426</sub> single mutant peptide
Chemical type: Peptide, no natural source
Sequence: LPFDRITVM
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 104.7

Epitope: PR8 NP<sub>418-426</sub> single mutant peptide
Chemical type: Peptide, no natural source
Sequence: LPFDRQTVM
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 95.5

Epitope: PR8 NP<sub>418-426</sub> single mutant peptide
Chemical type: Peptide, no natural source
Sequence: LPFDRTITVM
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 83.2

Epitope: PR8 NP<sub>418-426</sub> single mutant peptide
Chemical type: Peptide, no natural source
Sequence: LPFDRTTVI
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 95.5