Reference: Matthew Lacorcia; Pushpak Bhattacharjee; Abby Foster; Melinda Y Hardy; Jason A Tye-Din; John A Karas; John M Wentworth; Fergus J Cameron; Stuart I Mannering. "BASTA, a simple whole-blood assay for measuring β cell antigen-specific CD4<sup>+</sup> T cell responses in type 1 diabetes." Science translational medicine (2025).

Epitope: C-peptide
Chemical type: Peptide from protein
Sequence: EAEDLQVGQVELGGGPGAGSLQ
Position: 57-78
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: C-peptide
Chemical type: Peptide from protein
Sequence: EAEDLQVGQVELGGGPGAGSLQ
Position: 57-78
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: C-peptide
Chemical type: Peptide from protein
Sequence: EAEDLQVGQVELGGGPGAGSLQ
Position: 57-78
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: C-peptide
Chemical type: Peptide from protein
Sequence: EAEDLQVGQVELGGGPGAGSLQ
Position: 57-78
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: C-peptide
Chemical type: Peptide from protein
Sequence: EAEDLQVGQVELGGGPGAGSLQ
Position: 57-78
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: C-peptide
Chemical type: Peptide from protein
Sequence: EAEDLQVGQVELGGGPGAGSLQ
Position: 57-78
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: C-peptide
Chemical type: Peptide from protein
Sequence: EAEDLQVGQVELGGGPGAGSLQ
Position: 57-78
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: C-peptide
Chemical type: Peptide from protein
Sequence: EAEDLQVGQVELGGGPGAGSLQ
Position: 57-78
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: C-peptide
Chemical type: Peptide from protein
Sequence: EAEDLQVGQVELGGGPGAGSLQ
Position: 57-78
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: C-peptide
Chemical type: Peptide from protein
Sequence: EAEDLQVGQVELGGGPGAGSLQ
Position: 57-78
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: C-peptide
Chemical type: Peptide from protein
Sequence: EAEDLQVGQVELGGGPGAGSLQ
Position: 57-78
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: C-peptide
Chemical type: Peptide from protein
Sequence: EAEDLQVGQVELGGGPGAGSLQ
Position: 57-78
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: C-peptide
Chemical type: Peptide from protein
Sequence: EAEDLQVGQVELGGGPGAGSLQ
Position: 57-78
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: C-peptide
Chemical type: Peptide from protein
Sequence: EAEDLQVGQVELGGGPGAGSLQ
Position: 57-78
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Signal peptide
Chemical type: Peptide from protein
Sequence: MALWMRLLPLLALLALWGPDPAAA
Position: 1-24
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Signal peptide
Chemical type: Peptide from protein
Sequence: MALWMRLLPLLALLALWGPDPAAA
Position: 1-24
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: B chain peptide
Chemical type: Peptide from protein
Sequence: FVNQHLCGSHLVEALYLVCGERGFFYTPKT
Position: 25-54
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: B chain peptide
Chemical type: Peptide from protein
Sequence: FVNQHLCGSHLVEALYLVCGERGFFYTPKT
Position: 25-54
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: A chain
Chemical type: Peptide from protein
Sequence: GIVEQCCTSICSLYQLENYCN
Position: 90-110
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: A chain
Chemical type: Peptide from protein
Sequence: GIVEQCCTSICSLYQLENYCN
Position: 90-110
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: RREAEDLQVGQVELGGGP
Chemical type: Peptide from protein
Sequence: RREAEDLQVGQVELGGGP
Position: 55-72
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: RREAEDLQVGQVELGGGP
Chemical type: Peptide from protein
Sequence: RREAEDLQVGQVELGGGP
Position: 55-72
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: LQVGQVELGGGPGAGSLQ
Chemical type: Peptide from protein
Sequence: LQVGQVELGGGPGAGSLQ
Position: 61-78
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ELGGGPGAGSLQPLALEG
Chemical type: Peptide from protein
Sequence: ELGGGPGAGSLQPLALEG
Position: 67-84
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: LLALLALWGPDPAAAFVNQHL
Chemical type: Peptide from protein
Sequence: LLALLALWGPDPAAAFVNQHL
Position: 10-30
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PDPAAAFVNQHLCGSHLV
Chemical type: Peptide from protein
Sequence: PDPAAAFVNQHLCGSHLV
Position: 19-36
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PLALEGSLQKRGIVEQCC
Chemical type: Peptide from protein
Sequence: PLALEGSLQKRGIVEQCC
Position: 79-96
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: SLQKRGIVEQCCTSICSL
Chemical type: Peptide from protein
Sequence: SLQKRGIVEQCCTSICSL
Position: 85-102
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FYTPKTRREAEDLQVGQV
Chemical type: Peptide from protein
Sequence: FYTPKTRREAEDLQVGQV
Position: 49-66
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FYTPKTRREAEDLQVGQV
Chemical type: Peptide from protein
Sequence: FYTPKTRREAEDLQVGQV
Position: 49-66
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo