Reference: Eric L Greidinger; Yun Juan Zang; Kimberly Jaimes; Laisel Martinez; Mehdi Nassiri; Robert W Hoffman. "CD4+ T cells target epitopes residing within the RNA-binding domain of the U1-70-kDa small nuclear ribonucleoprotein autoantigen and have restricted TCR diversity in an HLA-DR4-transgenic murine model of mixed connective tissue disease." Journal of immunology (Baltimore, Md. : 1950) (2008).

Epitope: 70K.M.1.15
Chemical type: Peptide from protein
Sequence: MTQFLPPNLLALFAP
Position: 1-15
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.P.7.15
Chemical type: Peptide from protein
Sequence: PNLLALFAPRDPIPY
Position: 7-21
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.F.13.15
Chemical type: Peptide from protein
Sequence: FAPRDPIPYLPPLEK
Position: 13-27
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.I.19.15
Chemical type: Peptide from protein
Sequence: IPYLPPLEKLPHEKH
Position: 19-33
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.L.25.15
Chemical type: Peptide from protein
Sequence: LEKLPHEKHHNQPYC
Position: 25-39
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.E.31.15
Chemical type: Peptide from protein
Sequence: EKHHNQPYCGIAPYI
Position: 31-45
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.P.37.15
Chemical type: Peptide from protein
Sequence: PYCGIAPYIREFEDP
Position: 37-51
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.P.43.15
Chemical type: Peptide from protein
Sequence: PYIREFEDPRDAPPP
Position: 43-57
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.E.49.15
Chemical type: Peptide from protein
Sequence: EDPRDAPPPTRAETR
Position: 49-63
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.P.57.15
Chemical type: Peptide from protein
Sequence: PTRAETREERMERKR
Position: 57-71
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70.K.E.61.15
Chemical type: Peptide from protein
Sequence: ETREERMERKRREKI
Position: 61-75
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.M.67.15
Chemical type: Peptide from protein
Sequence: MERKRREKIERRQQE
Position: 67-81
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.E.73.15
Chemical type: Peptide from protein
Sequence: EKIERRQQEVETELK
Position: 73-87
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.E.81.15
Chemical type: Peptide from protein
Sequence: EVETELKMWDPHNDP
Position: 81-95
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.K.87.15
Chemical type: Peptide from protein
Sequence: KMWDPHNDPNAQGDA
Position: 87-101
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.N.93.15
Chemical type: Peptide from protein
Sequence: NDPNAQGDAFKTLFV
Position: 93-107
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.G.99.15
Chemical type: Peptide from protein
Sequence: GDAFKTLFVARVNYD
Position: 99-113
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.D.113.15
Chemical type: Peptide from protein
Sequence: DTTESKLRREFEVYG
Position: 113-127
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.R.121.15
Chemical type: Peptide from protein
Sequence: REFEVYGPIKRIHMV
Position: 121-135
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.I.132.15
Chemical type: Peptide from protein
Sequence: IHMVYSKRSGKPRGY
Position: 132-146
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.R.139.15
Chemical type: Peptide from protein
Sequence: RSGKPRGYAFIEYEH
Position: 139-153
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.A.147.15
Chemical type: Peptide from protein
Sequence: AFIEYEHERDMHSAY
Position: 147-161
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.H.153.15
Chemical type: Peptide from protein
Sequence: HERDMHSAYKHADGK
Position: 153-167
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.S.159.15
Chemical type: Peptide from protein
Sequence: SAYKHADGKKIDGRR
Position: 159-173
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.D.165.15
Chemical type: Peptide from protein
Sequence: DGKKIDGRRVLVDVE
Position: 165-179
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.I.169.15
Chemical type: Peptide from protein
Sequence: IDGRRVLVDVERGRT
Position: 169-183
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.R.173.15
Chemical type: Peptide from protein
Sequence: RVLVDVERGRTVKGW
Position: 173-187
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.K.185.15
Chemical type: Peptide from protein
Sequence: KGWRPRRLGGGLGGT
Position: 185-199
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.G.195.15
Chemical type: Peptide from protein
Sequence: GLGGTRRGGADVNIR
Position: 195-209
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.N.207.15
Chemical type: Peptide from protein
Sequence: NIRHSGRDDTSRYDE
Position: 207-221
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.Y.219.15
Chemical type: Peptide from protein
Sequence: YDERPGPSPLPHRDR
Position: 219-233
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.R.231.15
Chemical type: Peptide from protein
Sequence: RDRDRDRERERRERS
Position: 231-245
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.R.237.15
Chemical type: Peptide from protein
Sequence: RERERRERSRERDKE
Position: 237-251
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.D.249.15
Chemical type: Peptide from protein
Sequence: DKERERRRSRSRDRR
Position: 249-263
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.R.255.15
Chemical type: Peptide from protein
Sequence: RRSRSRDRRRRSRSR
Position: 255-269
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.D.261.15
Chemical type: Peptide from protein
Sequence: DRRRRSRSRDKEERR
Position: 261-275
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.R.267.15
Chemical type: Peptide from protein
Sequence: RSRDKEERRRSRERS
Position: 267-281
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.E.273.15
Chemical type: Peptide from protein
Sequence: ERRRSRERSKDKDRD
Position: 273-287
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.R.291.15
Chemical type: Peptide from protein
Sequence: RSSRSRERARRERER
Position: 291-305
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.E.297.15
Chemical type: Peptide from protein
Sequence: ERARRERERKEELRG
Position: 297-311
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.R.303.15
Chemical type: Peptide from protein
Sequence: RERKEELRGGGGDMA
Position: 303-317
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.L.309.15
Chemical type: Peptide from protein
Sequence: LRGGGGDMAEPSEAG
Position: 309-323
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.D.315.15
Chemical type: Peptide from protein
Sequence: DMAEPSEAGDAPPDD
Position: 315-329
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.E.321.15
Chemical type: Peptide from protein
Sequence: EAGDAPPDDGPPGEL
Position: 321-335
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.P.327.15
Chemical type: Peptide from protein
Sequence: PDDGPPGELGPDGPD
Position: 327-341
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.G.333.15
Chemical type: Peptide from protein
Sequence: GELGPDGPDGPEEKG
Position: 333-347
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.G.339.15
Chemical type: Peptide from protein
Sequence: GPDGPEEKGRDRDRE
Position: 339-353
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.E.345.15
Chemical type: Peptide from protein
Sequence: EKGRDRDRERRRSHR
Position: 345-359
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.S.357.15
Chemical type: Peptide from protein
Sequence: SHRSERERRRDRDRD
Position: 357-371
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.E.363.15
Chemical type: Peptide from protein
Sequence: ERRRDRDRDRDRDRE
Position: 363-377
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.D.369.15
Chemical type: Peptide from protein
Sequence: DRDRDRDREHKRGER
Position: 369-383
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.D.375.15
Chemical type: Peptide from protein
Sequence: DREHKRGERGSERGR
Position: 375-389
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.G.381.15
Chemical type: Peptide from protein
Sequence: GERGSERGRDEARGG
Position: 381-395
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.R.387.15
Chemical type: Peptide from protein
Sequence: RGRDEARGGGGGQDN
Position: 387-401
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.R.393.15
Chemical type: Peptide from protein
Sequence: RGGGGGQDNGLEGLG
Position: 393-407
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.Q.399.15
Chemical type: Peptide from protein
Sequence: QDNGLEGLGNDSRDM
Position: 399-413
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.G.405.15
Chemical type: Peptide from protein
Sequence: GLGNDSRDMYMESEG
Position: 405-419
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.R.411.15
Chemical type: Peptide from protein
Sequence: RDMYMESEGGDGYLA
Position: 411-425
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: 70K.Y.423.15
Chemical type: Peptide from protein
Sequence: YLAPENGYLMEAAPE
Position: 423-437
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: Epitope 2
Chemical type: Peptide from protein
Sequence: YDTTESKLRREFEVYGPIKRIHMVY
Position: 112-136
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
MHC allele types: 52 63 437 425 424 57
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Epitope 2
Chemical type: Peptide from protein
Sequence: YDTTESKLRREFEVYGPIKRIHMVY
Position: 112-136
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: Epitope 3
Chemical type: Peptide from protein
Sequence: HMVYSKRSGKPR
Position: 133-144
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
MHC allele types: 52 63 437 425 424 57
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Epitope 3
Chemical type: Peptide from protein
Sequence: HMVYSKRSGKPR
Position: 133-144
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: Epitope 4
Chemical type: Peptide from protein
Sequence: YEHERDMHSAYKHAD
Position: 151-165
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
MHC allele types: 52 63 437 425 424 57
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Epitope 4
Chemical type: Peptide from protein
Sequence: YEHERDMHSAYKHAD
Position: 151-165
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: Epitope 5
Chemical type: Peptide from protein
Sequence: VLVDVERGRTV
Position: 174-184
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: Epitope 5
Chemical type: Peptide from protein
Sequence: VLVDVERGRTV
Position: 174-184
Source molecule (GenBank): P08621.2
Assay category: TCell
Result: Positive
MHC allele types: 52 63 437 425 424 57
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo