Reference: Marie Eliane Azoury; Mahmoud Tarayrah; Georgia Afonso; Aurore Pais; Maikel L Colli; Claire Maillard; Cassandra Lavaud; Laure Alexandre-Heymann; Sergio Gonzalez-Duque; Yann Verdier; Joelle Vinh; Sheena Pinto; Soren Buus; Danièle Dubois-Laforgue; Etienne Larger; Jean-Paul Beressi; Graziella Bruno; Decio L Eizirik; Sylvaine You; Roberto Mallone. "Peptides Derived From Insulin Granule Proteins Are Targeted by CD8<sup>+</sup> T Cells Across MHC Class I Restrictions in Humans and NOD Mice." Diabetes (2020).

Epitope: ABCC8 787-795
Chemical type: Peptide from protein
Sequence: IIFESPFNK
Position: 787-795
Source molecule (GenBank): Q09428.6
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: ABCC8 787-795
Chemical type: Peptide from protein
Sequence: IIFESPFNK
Position: 787-795
Source molecule (GenBank): Q09428.6
Assay category: MhcLigandElution
Result: Positive

Epitope: ABCC8 787-795
Chemical type: Peptide from protein
Sequence: IIFESPFNK
Position: 787-795
Source molecule (GenBank): Q09428.6
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 238.2

Epitope: INS/PTPRN 15-21/893-896
Chemical type: Peptide from protein
Sequence: ALWGPDPKVNK
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: INS/PTPRN 15-21/893-896
Chemical type: Peptide from protein
Sequence: ALWGPDPKVNK
Assay category: MhcLigandElution
Result: Positive

Epitope: INS/PTPRN 15-21/893-896
Chemical type: Peptide from protein
Sequence: ALWGPDPKVNK
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 111.8

Epitope: KCNK16 13-21
Chemical type: Peptide from protein
Sequence: RVLPLLLAY
Position: 13-21
Source molecule (GenBank): Q96T55.1
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: KCNK16 13-21
Chemical type: Peptide from protein
Sequence: RVLPLLLAY
Position: 13-21
Source molecule (GenBank): Q96T55.1
Assay category: MhcLigandElution
Result: Positive

Epitope: KCNK16 13-21
Chemical type: Peptide from protein
Sequence: RVLPLLLAY
Position: 13-21
Source molecule (GenBank): Q96T55.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 147.8

Epitope: KIF1A/B/C 153-161
Chemical type: Peptide from protein
Sequence: RVRDLLNPK
Position: 153-161
Source molecule (GenBank): Q12756.2
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: KIF1A/B/C 153-161
Chemical type: Peptide from protein
Sequence: RVRDLLNPK
Position: 153-161
Source molecule (GenBank): Q12756.2
Assay category: MhcLigandElution
Result: Positive

Epitope: KIF1A/B/C 153-161
Chemical type: Peptide from protein
Sequence: RVRDLLNPK
Position: 153-161
Source molecule (GenBank): Q12756.2
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 142.3

Epitope: KIF1A 219-228
Chemical type: Peptide from protein
Sequence: AVFNIIFTQK
Position: 219-228
Source molecule (GenBank): Q12756.2
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: KIF1A 219-228
Chemical type: Peptide from protein
Sequence: AVFNIIFTQK
Position: 219-228
Source molecule (GenBank): Q12756.2
Assay category: MhcLigandElution
Result: Positive

Epitope: KIF1A 219-228
Chemical type: Peptide from protein
Sequence: AVFNIIFTQK
Position: 219-228
Source molecule (GenBank): Q12756.2
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 105.5

Epitope: KIF1A 860-868
Chemical type: Peptide from protein
Sequence: LLYPVPLVH
Position: 860-868
Source molecule (GenBank): Q12756.2
Assay category: TCell
Result: Positive
Host:  22-40 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: KIF1A 860-868
Chemical type: Peptide from protein
Sequence: LLYPVPLVH
Position: 860-868
Source molecule (GenBank): Q12756.2
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: KIF1A 860-868
Chemical type: Peptide from protein
Sequence: LLYPVPLVH
Position: 860-868
Source molecule (GenBank): Q12756.2
Assay category: MhcLigandElution
Result: Positive

Epitope: KIF1A 860-868
Chemical type: Peptide from protein
Sequence: LLYPVPLVH
Position: 860-868
Source molecule (GenBank): Q12756.2
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 96.7

Epitope: PCSK1 706-714
Chemical type: Peptide from protein
Sequence: KLNKPSQLK
Position: 706-714
Source molecule (GenBank): NP_000430.3
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: PCSK1 706-714
Chemical type: Peptide from protein
Sequence: KLNKPSQLK
Position: 706-714
Source molecule (GenBank): NP_000430.3
Assay category: MhcLigandElution
Result: Positive

Epitope: PCSK1 706-714
Chemical type: Peptide from protein
Sequence: KLNKPSQLK
Position: 706-714
Source molecule (GenBank): NP_000430.3
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 109.9

Epitope: PNMA2 50-58
Chemical type: Peptide from protein
Sequence: RLLGKIFRK
Position: 50-58
Source molecule (GenBank): Q9UL42.2
Assay category: TCell
Result: Negative
Host:  22-40 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: PNMA2 50-58
Chemical type: Peptide from protein
Sequence: RLLGKIFRK
Position: 50-58
Source molecule (GenBank): Q9UL42.2
Assay category: TCell
Result: Negative
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: PNMA2 50-58
Chemical type: Peptide from protein
Sequence: RLLGKIFRK
Position: 50-58
Source molecule (GenBank): Q9UL42.2
Assay category: MhcLigandElution
Result: Positive

Epitope: PNMA2 50-58
Chemical type: Peptide from protein
Sequence: RLLGKIFRK
Position: 50-58
Source molecule (GenBank): Q9UL42.2
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 179.2

Epitope: PTPRN;PTPRN2 965-975
Chemical type: Peptide from protein
Sequence: AVAEEVNAILK
Position: 965-975
Source molecule (GenBank): Q16849.1
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: PTPRN;PTPRN2 965-975
Chemical type: Peptide from protein
Sequence: AVAEEVNAILK
Position: 965-975
Source molecule (GenBank): Q16849.1
Assay category: MhcLigandElution
Result: Positive

Epitope: PTPRN;PTPRN2 965-975
Chemical type: Peptide from protein
Sequence: AVAEEVNAILK
Position: 965-975
Source molecule (GenBank): Q16849.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 119.6

Epitope: RTN1 120-129
Chemical type: Peptide from protein
Sequence: STYFTGILQK
Position: 120-129
Source molecule (GenBank): Q16799.1
Assay category: TCell
Result: Positive
Host:  22-40 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: RTN1 120-129
Chemical type: Peptide from protein
Sequence: STYFTGILQK
Position: 120-129
Source molecule (GenBank): Q16799.1
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: RTN1 120-129
Chemical type: Peptide from protein
Sequence: STYFTGILQK
Position: 120-129
Source molecule (GenBank): Q16799.1
Assay category: MhcLigandElution
Result: Positive

Epitope: RTN1 120-129
Chemical type: Peptide from protein
Sequence: STYFTGILQK
Position: 120-129
Source molecule (GenBank): Q16799.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 114.1

Epitope: SCG3 166-174
Chemical type: Peptide from protein
Sequence: AVFDKIVSK
Position: 166-174
Source molecule (GenBank): Q8WXD2.3
Assay category: TCell
Result: Positive
Host:  22-40 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: SCG3 166-174
Chemical type: Peptide from protein
Sequence: AVFDKIVSK
Position: 166-174
Source molecule (GenBank): Q8WXD2.3
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: SCG3 166-174
Chemical type: Peptide from protein
Sequence: AVFDKIVSK
Position: 166-174
Source molecule (GenBank): Q8WXD2.3
Assay category: MhcLigandElution
Result: Positive

Epitope: SCG3 166-174
Chemical type: Peptide from protein
Sequence: AVFDKIVSK
Position: 166-174
Source molecule (GenBank): Q8WXD2.3
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 213.8

Epitope: SCG5-009 193-201
Chemical type: Peptide from protein
Sequence: RLKPSLVGK
Position: 193-201
Source molecule (GenBank): H7C4X7.1
Assay category: TCell
Result: Positive
Host:  22-40 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: SCG5-009 193-201
Chemical type: Peptide from protein
Sequence: RLKPSLVGK
Position: 193-201
Source molecule (GenBank): H7C4X7.1
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: SCG5-009 193-201
Chemical type: Peptide from protein
Sequence: RLKPSLVGK
Position: 193-201
Source molecule (GenBank): H7C4X7.1
Assay category: MhcLigandElution
Result: Positive

Epitope: SCG5-009 193-201
Chemical type: Peptide from protein
Sequence: RLKPSLVGK
Position: 193-201
Source molecule (GenBank): H7C4X7.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 150.3

Epitope: SLC30A8 6-16
Chemical type: Peptide from protein
Sequence: RTYLVNDKAAK
Position: 6-16
Source molecule (GenBank): Q8IWU4.2
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: SLC30A8 6-16
Chemical type: Peptide from protein
Sequence: RTYLVNDKAAK
Position: 6-16
Source molecule (GenBank): Q8IWU4.2
Assay category: MhcLigandElution
Result: Positive

Epitope: SLC30A8 6-16
Chemical type: Peptide from protein
Sequence: RTYLVNDKAAK
Position: 6-16
Source molecule (GenBank): Q8IWU4.2
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 118.8

Epitope: SLC30A8/NPY;SLC30A8/CHGA 16-21/90-92;16-21/357-359
Chemical type: Peptide from protein
Sequence: KMYAFTRLE
Assay category: MhcLigandElution
Result: Positive

Epitope: SLC30A8/NPY;SLC30A8/CHGA 16-21/90-92;16-21/357-359
Chemical type: Peptide from protein
Sequence: KMYAFTRLE
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 2.5

Epitope: UCN3 46-56
Chemical type: Peptide from protein
Sequence: GQWEDASLLSK
Position: 46-56
Source molecule (GenBank): Q969E3.2
Assay category: TCell
Result: Positive
Host:  22-40 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: UCN3 46-56
Chemical type: Peptide from protein
Sequence: GQWEDASLLSK
Position: 46-56
Source molecule (GenBank): Q969E3.2
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: UCN3 46-56
Chemical type: Peptide from protein
Sequence: GQWEDASLLSK
Position: 46-56
Source molecule (GenBank): Q969E3.2
Assay category: MhcLigandElution
Result: Positive

Epitope: UCN3 46-56
Chemical type: Peptide from protein
Sequence: GQWEDASLLSK
Position: 46-56
Source molecule (GenBank): Q969E3.2
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 90.8

Epitope: C15orf48-003 50-58
Chemical type: Peptide from protein
Sequence: KVSAPAYCK
Assay category: TCell
Result: Positive
Host:  22-40 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: C15orf48-003 50-58
Chemical type: Peptide from protein
Sequence: KVSAPAYCK
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: C15orf48-003 50-58
Chemical type: Peptide from protein
Sequence: KVSAPAYCK
Assay category: MhcLigandElution
Result: Positive

Epitope: C15orf48-003 50-58
Chemical type: Peptide from protein
Sequence: KVSAPAYCK
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 102.7

Epitope: C15orf48-003 97-106
Chemical type: Peptide from protein
Sequence: ATGRSQMVRK
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: C15orf48-003 97-106
Chemical type: Peptide from protein
Sequence: ATGRSQMVRK
Assay category: MhcLigandElution
Result: Positive

Epitope: C15orf48-003 97-106
Chemical type: Peptide from protein
Sequence: ATGRSQMVRK
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 32.9

Epitope: C15orf48-003 103-111
Chemical type: Peptide from protein
Sequence: MVRKGMNLK
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: C15orf48-003 103-111
Chemical type: Peptide from protein
Sequence: MVRKGMNLK
Assay category: MhcLigandElution
Result: Positive

Epitope: C15orf48-003 103-111
Chemical type: Peptide from protein
Sequence: MVRKGMNLK
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 83.8

Epitope: CTNND1-026 852-860
Chemical type: Peptide from protein
Sequence: QVSYPSMQK
Position: 852-860
Source molecule (GenBank): NP_001078933.1
Assay category: TCell
Result: Positive
Host:  22-40 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: CTNND1-026 852-860
Chemical type: Peptide from protein
Sequence: QVSYPSMQK
Position: 852-860
Source molecule (GenBank): NP_001078933.1
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: CTNND1-026 852-860
Chemical type: Peptide from protein
Sequence: QVSYPSMQK
Position: 852-860
Source molecule (GenBank): NP_001078933.1
Assay category: MhcLigandElution
Result: Positive

Epitope: CTNND1-026 852-860
Chemical type: Peptide from protein
Sequence: QVSYPSMQK
Position: 852-860
Source molecule (GenBank): NP_001078933.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 72.5

Epitope: G6PC2-001 317-326
Chemical type: Peptide from protein
Sequence: HLFYVLSFCK
Position: 318-327
Source molecule (GenBank): NP_066999.1
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: G6PC2-001 317-326
Chemical type: Peptide from protein
Sequence: HLFYVLSFCK
Position: 318-327
Source molecule (GenBank): NP_066999.1
Assay category: MhcLigandElution
Result: Positive

Epitope: G6PC2-001 317-326
Chemical type: Peptide from protein
Sequence: HLFYVLSFCK
Position: 318-327
Source molecule (GenBank): NP_066999.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 37.3

Epitope: G6PC2-001 343-351
Chemical type: Peptide from protein
Sequence: HMLMKQSGK
Position: 344-352
Source molecule (GenBank): NP_066999.1
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: G6PC2-001 343-351
Chemical type: Peptide from protein
Sequence: HMLMKQSGK
Position: 344-352
Source molecule (GenBank): NP_066999.1
Assay category: MhcLigandElution
Result: Positive

Epitope: G6PC2-001 343-351
Chemical type: Peptide from protein
Sequence: HMLMKQSGK
Position: 344-352
Source molecule (GenBank): NP_066999.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 79.5

Epitope: GNAS-036 22-31
Chemical type: Peptide from protein
Sequence: KVKKVPLAEK
Position: 8-17
Source molecule (GenBank): Q5JWE9
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: GNAS-036 22-31
Chemical type: Peptide from protein
Sequence: KVKKVPLAEK
Position: 8-17
Source molecule (GenBank): Q5JWE9
Assay category: MhcLigandElution
Result: Positive

Epitope: GNAS-036 22-31
Chemical type: Peptide from protein
Sequence: KVKKVPLAEK
Position: 8-17
Source molecule (GenBank): Q5JWE9
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 220.6

Epitope: GNAS-036 33-42
Chemical type: Peptide from protein
Sequence: RQMRKEALEK
Position: 19-28
Source molecule (GenBank): Q5JWE9
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: GNAS-036 33-42
Chemical type: Peptide from protein
Sequence: RQMRKEALEK
Position: 19-28
Source molecule (GenBank): Q5JWE9
Assay category: MhcLigandElution
Result: Positive

Epitope: GNAS-036 33-42
Chemical type: Peptide from protein
Sequence: RQMRKEALEK
Position: 19-28
Source molecule (GenBank): Q5JWE9
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 34.2

Epitope: GNAS-036 74-83
Chemical type: Peptide from protein
Sequence: LLLGAGESGK
Position: 60-69
Source molecule (GenBank): Q5JWE9
Assay category: TCell
Result: Positive
Host:  22-40 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: GNAS-036 74-83
Chemical type: Peptide from protein
Sequence: LLLGAGESGK
Position: 60-69
Source molecule (GenBank): Q5JWE9
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: GNAS-036 74-83
Chemical type: Peptide from protein
Sequence: LLLGAGESGK
Position: 60-69
Source molecule (GenBank): Q5JWE9
Assay category: MhcLigandElution
Result: Positive

Epitope: GNAS-036 74-83
Chemical type: Peptide from protein
Sequence: LLLGAGESGK
Position: 60-69
Source molecule (GenBank): Q5JWE9
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 4.4

Epitope: GNAS-050 16-24
Chemical type: Peptide from protein
Sequence: RSSTRHSGK
Position: 17-25
Source molecule (GenBank): NP_001070958.1
Assay category: TCell
Result: Positive-Low
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: GNAS-050 16-24
Chemical type: Peptide from protein
Sequence: RSSTRHSGK
Position: 17-25
Source molecule (GenBank): NP_001070958.1
Assay category: MhcLigandElution
Result: Positive

Epitope: GNAS-050 16-24
Chemical type: Peptide from protein
Sequence: RSSTRHSGK
Position: 17-25
Source molecule (GenBank): NP_001070958.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 87.5

Epitope: GNAS-050 159-168
Chemical type: Peptide from protein
Sequence: RLCHLSLMEK
Position: 160-169
Source molecule (GenBank): NP_001070958.1
Assay category: TCell
Result: Positive
Host:  22-40 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: GNAS-050 159-168
Chemical type: Peptide from protein
Sequence: RLCHLSLMEK
Position: 160-169
Source molecule (GenBank): NP_001070958.1
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: GNAS-050 159-168
Chemical type: Peptide from protein
Sequence: RLCHLSLMEK
Position: 160-169
Source molecule (GenBank): NP_001070958.1
Assay category: MhcLigandElution
Result: Positive

Epitope: GNAS-050 159-168
Chemical type: Peptide from protein
Sequence: RLCHLSLMEK
Position: 160-169
Source molecule (GenBank): NP_001070958.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 88.7

Epitope: GNAS-050 477-485
Chemical type: Peptide from protein
Sequence: RLLRGLLAR
Position: 478-486
Source molecule (GenBank): NP_001070958.1
Assay category: TCell
Result: Positive
Host:  22-40 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: GNAS-050 477-485
Chemical type: Peptide from protein
Sequence: RLLRGLLAR
Position: 478-486
Source molecule (GenBank): NP_001070958.1
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: GNAS-050 477-485
Chemical type: Peptide from protein
Sequence: RLLRGLLAR
Position: 478-486
Source molecule (GenBank): NP_001070958.1
Assay category: MhcLigandElution
Result: Positive

Epitope: GNAS-050 477-485
Chemical type: Peptide from protein
Sequence: RLLRGLLAR
Position: 478-486
Source molecule (GenBank): NP_001070958.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 53.9

Epitope: GNAS-050 496-504
Chemical type: Peptide from protein
Sequence: AAAACTTMK
Position: 497-505
Source molecule (GenBank): NP_001070958.1
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: GNAS-050 496-504
Chemical type: Peptide from protein
Sequence: AAAACTTMK
Position: 497-505
Source molecule (GenBank): NP_001070958.1
Assay category: MhcLigandElution
Result: Positive

Epitope: GNAS-050 496-504
Chemical type: Peptide from protein
Sequence: AAAACTTMK
Position: 497-505
Source molecule (GenBank): NP_001070958.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 53.2

Epitope: GNAS-050 542-551
Chemical type: Peptide from protein
Sequence: ATYSATFSCK
Position: 543-552
Source molecule (GenBank): NP_001070958.1
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: GNAS-050 542-551
Chemical type: Peptide from protein
Sequence: ATYSATFSCK
Position: 543-552
Source molecule (GenBank): NP_001070958.1
Assay category: MhcLigandElution
Result: Positive

Epitope: GNAS-050 542-551
Chemical type: Peptide from protein
Sequence: ATYSATFSCK
Position: 543-552
Source molecule (GenBank): NP_001070958.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 63.8

Epitope: LARP4-006 222-230
Chemical type: Peptide from protein
Sequence: KAINTFFAK
Position: 272-280
Source molecule (GenBank): Q71RC2.3
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: LARP4-006 222-230
Chemical type: Peptide from protein
Sequence: KAINTFFAK
Position: 272-280
Source molecule (GenBank): Q71RC2.3
Assay category: MhcLigandElution
Result: Positive

Epitope: LARP4-006 222-230
Chemical type: Peptide from protein
Sequence: KAINTFFAK
Position: 272-280
Source molecule (GenBank): Q71RC2.3
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 83.5

Epitope: MAP3K13-012 33-41
Chemical type: Peptide from protein
Sequence: AMGNHPSPK
Position: 33-41
Source molecule (GenBank): O43283.1
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: MAP3K13-012 33-41
Chemical type: Peptide from protein
Sequence: AMGNHPSPK
Position: 33-41
Source molecule (GenBank): O43283.1
Assay category: MhcLigandElution
Result: Positive

Epitope: MAP3K13-012 33-41
Chemical type: Peptide from protein
Sequence: AMGNHPSPK
Position: 33-41
Source molecule (GenBank): O43283.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 83.2

Epitope: MNX1-001 217-225
Chemical type: Peptide from protein
Sequence: STAGMILPK
Position: 217-225
Source molecule (GenBank): P50219.3
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: MNX1-001 217-225
Chemical type: Peptide from protein
Sequence: STAGMILPK
Position: 217-225
Source molecule (GenBank): P50219.3
Assay category: MhcLigandElution
Result: Positive

Epitope: MNX1-001 217-225
Chemical type: Peptide from protein
Sequence: STAGMILPK
Position: 217-225
Source molecule (GenBank): P50219.3
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 23.1

Epitope: ONECUT1-003 5-14
Chemical type: Peptide from protein
Sequence: KSFIKHAKGK
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: ONECUT1-003 5-14
Chemical type: Peptide from protein
Sequence: KSFIKHAKGK
Assay category: MhcLigandElution
Result: Positive

Epitope: ONECUT1-003 5-14
Chemical type: Peptide from protein
Sequence: KSFIKHAKGK
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 11.5

Epitope: ONECUT1-003 47-56
Chemical type: Peptide from protein
Sequence: KISVHPKNCK
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: ONECUT1-003 47-56
Chemical type: Peptide from protein
Sequence: KISVHPKNCK
Assay category: MhcLigandElution
Result: Positive

Epitope: ONECUT1-003 47-56
Chemical type: Peptide from protein
Sequence: KISVHPKNCK
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 63.8

Epitope: PDIA2-007 86-94
Chemical type: Peptide from protein
Sequence: AVHGFPTLK
Position: 457-465
Source molecule (GenBank): Q13087.2
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: PDIA2-007 86-94
Chemical type: Peptide from protein
Sequence: AVHGFPTLK
Position: 457-465
Source molecule (GenBank): Q13087.2
Assay category: MhcLigandElution
Result: Positive

Epitope: PDIA2-007 86-94
Chemical type: Peptide from protein
Sequence: AVHGFPTLK
Position: 457-465
Source molecule (GenBank): Q13087.2
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 89.3

Epitope: PFN2-008 59-67
Chemical type: Peptide from protein
Sequence: RVLVFVMGK
Position: 108-116
Source molecule (GenBank): P35080.3
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: PFN2-008 59-67
Chemical type: Peptide from protein
Sequence: RVLVFVMGK
Position: 108-116
Source molecule (GenBank): P35080.3
Assay category: MhcLigandElution
Result: Positive

Epitope: PFN2-008 59-67
Chemical type: Peptide from protein
Sequence: RVLVFVMGK
Position: 108-116
Source molecule (GenBank): P35080.3
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 21.8

Epitope: PRKACB-008 2-10
Chemical type: Peptide from protein
Sequence: GLLKEFLAK
Position: 2-10
Source molecule (GenBank): NP_001287846.1
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: PRKACB-008 2-10
Chemical type: Peptide from protein
Sequence: GLLKEFLAK
Position: 2-10
Source molecule (GenBank): NP_001287846.1
Assay category: MhcLigandElution
Result: Positive

Epitope: PRKACB-008 2-10
Chemical type: Peptide from protein
Sequence: GLLKEFLAK
Position: 2-10
Source molecule (GenBank): NP_001287846.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 61.9

Epitope: REXO2-020 2-11
Chemical type: Peptide from protein
Sequence: SVANALWIVK
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: REXO2-020 2-11
Chemical type: Peptide from protein
Sequence: SVANALWIVK
Assay category: MhcLigandElution
Result: Positive

Epitope: REXO2-020 2-11
Chemical type: Peptide from protein
Sequence: SVANALWIVK
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 11.1

Epitope: ZNF268-015 86-95
Chemical type: Peptide from protein
Sequence: ILKAGKSKAK
Position: 86-95
Source molecule (GenBank): NP_001159359.1
Assay category: TCell
Result: Positive
Host:  24-47 years
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Direct Ex Vivo

Epitope: ZNF268-015 86-95
Chemical type: Peptide from protein
Sequence: ILKAGKSKAK
Position: 86-95
Source molecule (GenBank): NP_001159359.1
Assay category: MhcLigandElution
Result: Positive

Epitope: ZNF268-015 86-95
Chemical type: Peptide from protein
Sequence: ILKAGKSKAK
Position: 86-95
Source molecule (GenBank): NP_001159359.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 102.1

Epitope: UCN3 5-13
Chemical type: Peptide from protein
Sequence: TYFLLPLLL
Position: 5-13
Source molecule (GenBank): Q924A4.1
Assay category: TCell
Result: Positive
Host: F 12-16 weeks
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Short Term Restimulated

Epitope: UCN3 32-40
Chemical type: Peptide from protein
Sequence: VFSCLNTAL
Position: 32-40
Source molecule (GenBank): Q924A4.1
Assay category: TCell
Result: Positive
Host: F 12-16 weeks
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Short Term Restimulated

Epitope: Pcsk2 109-118
Chemical type: Peptide from protein
Sequence: GYRDINEIDI
Position: 109-118
Source molecule (GenBank): P21661.1
Assay category: TCell
Result: Positive
Host: F 12-16 weeks
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Short Term Restimulated

Epitope: Pcsk2 341-350
Chemical type: Peptide from protein
Sequence: LYDESCSSTL
Position: 341-350
Source molecule (GenBank): P21661.1
Assay category: TCell
Result: Positive
Host: F 12-16 weeks
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Short Term Restimulated

Epitope: Pcsk2 501-510
Chemical type: Peptide from protein
Sequence: RYLEHVQAVI
Position: 501-510
Source molecule (GenBank): P21661.1
Assay category: TCell
Result: Positive
Host: F 12-16 weeks
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Short Term Restimulated

Epitope: Scg5 4-13
Chemical type: Peptide from protein
Sequence: RLVSAMLSGL
Position: 4-13
Source molecule (GenBank): P12961.1
Assay category: TCell
Result: Positive
Host: F 12-16 weeks
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Short Term Restimulated

Epitope: Scg5 8-17
Chemical type: Peptide from protein
Sequence: AMLSGLLFWL
Position: 8-17
Source molecule (GenBank): P12961.1
Assay category: TCell
Result: Positive
Host: F 12-16 weeks
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Short Term Restimulated

Epitope: Scg5 26-35
Chemical type: Peptide from protein
Sequence: AYSPRTPDRV
Position: 26-35
Source molecule (GenBank): P12961.1
Assay category: TCell
Result: Positive
Host: F 12-16 weeks
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Short Term Restimulated

Epitope: Scg5 193-201
Chemical type: Peptide from protein
Sequence: DNVVAKKSV
Position: 193-201
Source molecule (GenBank): P12961.1
Assay category: TCell
Result: Positive
Host: F 12-16 weeks
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Short Term Restimulated

Epitope: Isl1 27-36
Chemical type: Peptide from protein
Sequence: QYILRVSPDL
Position: 27-36
Source molecule (GenBank): P61372.1
Assay category: TCell
Result: Positive
Host: F 12-16 weeks
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Short Term Restimulated

Epitope: Isl1 65-74
Chemical type: Peptide from protein
Sequence: TYCKRDYIRL
Position: 65-74
Source molecule (GenBank): P61372.1
Assay category: TCell
Result: Positive
Host: F 12-16 weeks
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Short Term Restimulated

Epitope: Isl1 286-294
Chemical type: Peptide from protein
Sequence: SYQPPWKVL
Position: 286-294
Source molecule (GenBank): P61372.1
Assay category: TCell
Result: Positive
Host: F 12-16 weeks
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Short Term Restimulated

Epitope: Tum 148-156
Chemical type: Peptide from protein
Sequence: KYQAVTTTL
Position: 148-156
Source molecule (GenBank): CAA35908.1
Assay category: TCell
Result: Negative
Host: F 12-16 weeks
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Short Term Restimulated

Epitope: Ins1/Ins2 B15-23
Chemical type: Peptide from protein
Sequence: LYLVCGERG
Position: 39-47
Source molecule (GenBank): P01326.1
Assay category: TCell
Result: Positive
Host: F 12-16 weeks
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Short Term Restimulated

Epitope: Igrp 206-214
Chemical type: Peptide from protein
Sequence: VYLKTNVFL
Position: 206-214
Source molecule (GenBank): NP_067306.1
Assay category: TCell
Result: Positive-High
Host: F 12-16 weeks
Cell type: T cell CD8+
Tissue: pancreas
Culture conditions: Short Term Restimulated