Reference: An-Ran Shen; Xiao-Xiao Jin; Tao-Tao Tang; Yan Ding; Xiao-Tao Liu; Xin Zhong; Yan-Dan Wu; Xue-Lian Han; Guang-Yu Zhao; Chuan-Lai Shen; Lin-Li Lv; Bi-Cheng Liu. "Exosomal Vaccine Loading T Cell Epitope Peptides of SARS-CoV-2 Induces Robust CD8<sup>+</sup> T Cell Response in HLA-A Transgenic Mice." International journal of nanomedicine (2022).

Epitope: A1
Chemical type: Peptide from protein
Sequence: FLAFVVFLL
Position: 20-28
Source molecule (GenBank): P0DTC4.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A3
Chemical type: Peptide from protein
Sequence: VLLFLAFVV
Position: 17-25
Source molecule (GenBank): P0DTC4.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A4
Chemical type: Peptide from protein
Sequence: FLLVTLAIL
Position: 26-34
Source molecule (GenBank): P0DTC4.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A5
Chemical type: Peptide from protein
Sequence: RLCAYCCNIV
Position: 38-47
Source molecule (GenBank): P0DTC4.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B1
Chemical type: Peptide from protein
Sequence: GLMWLSYFI
Position: 89-97
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B2
Chemical type: Peptide from protein
Sequence: KLLEQWNLV
Position: 15-23
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B4
Chemical type: Peptide from protein
Sequence: FLFLTWICLL
Position: 26-35
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B3
Chemical type: Peptide from protein
Sequence: FVLAAVYRI
Position: 65-73
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: C1
Chemical type: Peptide from protein
Sequence: LLLDRLNQL
Position: 222-230
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: C2
Chemical type: Peptide from protein
Sequence: GMSRIGMEV
Position: 316-324
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: C3
Chemical type: Peptide from protein
Sequence: WLTYTGAIKL
Position: 330-339
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D2
Chemical type: Peptide from protein
Sequence: FIAGLIAIV
Position: 1220-1228
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D5
Chemical type: Peptide from protein
Sequence: KLNDLCFTNV
Position: 386-395
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D7
Chemical type: Peptide from protein
Sequence: VLNDILSRL
Position: 976-984
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D12
Chemical type: Peptide from protein
Sequence: FVFLVLLPLV
Position: 2-11
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D13
Chemical type: Peptide from protein
Sequence: MIAQYTSAL
Position: 869-877
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D11
Chemical type: Peptide from protein
Sequence: VVFLHVTYV
Position: 1060-1068
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: R5
Chemical type: Peptide from protein
Sequence: SLAIDAYPL
Position: 5253-5261
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: R6
Chemical type: Peptide from protein
Sequence: NLLKDCPAV
Position: 4480-4488
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: R8
Chemical type: Peptide from protein
Sequence: FVNEFYAYL
Position: 5133-5141
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: R11
Chemical type: Peptide from protein
Sequence: VMCGGSLYV
Position: 5059-5067
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Negative
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: R12
Chemical type: Peptide from protein
Sequence: MLDMYSVML
Position: 5291-5299
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Negative
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: R14
Chemical type: Peptide from protein
Sequence: RLANECAQV
Position: 5046-5054
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: R15
Chemical type: Peptide from protein
Sequence: QLLFVVEVV
Position: 4860-4868
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: R3
Chemical type: Peptide from protein
Sequence: LMIERFVSL
Position: 5246-5254
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Negative
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: R4
Chemical type: Peptide from protein
Sequence: AMRNAGIVGV
Position: 4587-4596
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: R9
Chemical type: Peptide from protein
Sequence: ILHCANFNV
Position: 4699-4707
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: R10
Chemical type: Peptide from protein
Sequence: KIFVDGVPFV
Position: 4724-4733
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: R13
Chemical type: Peptide from protein
Sequence: NMLRIMASL
Position: 5020-5028
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Host: M 8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo