Reference: M T Valle; A M Megiovanni; A Merlo; G Li Pira; L Bottone; G Angelini; L Bracci; L Lozzi; K Huygen; F Manca. "Epitope focus, clonal composition and Th1 phenotype of the human CD4 response to the secretory mycobacterial antigen Ag85." Clinical and experimental immunology (2001).

Epitope: Peptide 11
Chemical type: Peptide from protein
Sequence: QTYKWETLLTSE
Position: 133-144
Source molecule (GenBank): AAA25359.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Peptide 11
Chemical type: Peptide from protein
Sequence: QTYKWETLLTSE
Position: 133-144
Source molecule (GenBank): AAA25359.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: Peptide 1
Chemical type: Peptide from protein
Sequence: FSRPGLPVEYLQVPSPSM
Position: 41-58
Source molecule (GenBank): AAA25359.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Peptide 2
Chemical type: Peptide from protein
Sequence: YLQVPSPSMGRDIKVQFQ
Position: 50-67
Source molecule (GenBank): AAA25359.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Peptide 3
Chemical type: Peptide from protein
Sequence: GRDIKVQFQSGGNNSPAV
Position: 59-76
Source molecule (GenBank): AAA25359.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Peptide 4
Chemical type: Peptide from protein
Sequence: SGGNNSPAVYLLDGLRAQ
Position: 68-85
Source molecule (GenBank): AAA25359.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Peptide 6
Chemical type: Peptide from protein
Sequence: DDYNGWDINTPAFEWYYQ
Position: 86-103
Source molecule (GenBank): AAA25359.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Peptide 7
Chemical type: Peptide from protein
Sequence: TPAFEWYYQSGLSIVMPV
Position: 95-112
Source molecule (GenBank): AAA25359.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Peptide 9
Chemical type: Peptide from protein
Sequence: GGQSSFYSDWYSPACGKA
Position: 113-130
Source molecule (GenBank): AAA25359.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Peptide 10
Chemical type: Peptide from protein
Sequence: WYSPACGKAGCQTYKWET
Position: 122-139
Source molecule (GenBank): AAA25359.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Peptide 12
Chemical type: Peptide from protein
Sequence: LLTSELPQWLSANRAVKP
Position: 140-157
Source molecule (GenBank): AAA25359.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Peptide 15
Chemical type: Peptide from protein
Sequence: LSMAGSSAMILAAYHPQQ
Position: 165-182
Source molecule (GenBank): AAA25359.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Peptide 16
Chemical type: Peptide from protein
Sequence: ILAAYHPQQFIYAGSLSA
Position: 174-191
Source molecule (GenBank): AAA25359.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Peptide 17
Chemical type: Peptide from protein
Sequence: FIYAGSLSALLDPSQGMG
Position: 183-200
Source molecule (GenBank): AAA25359.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Peptide 19
Chemical type: Peptide from protein
Sequence: LIGLAMGDAGGYKAADMW
Position: 201-218
Source molecule (GenBank): AAA25359.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Peptide 20
Chemical type: Peptide from protein
Sequence: GGYKAADMWGPSSDPAWE
Position: 210-227
Source molecule (GenBank): AAA25359.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Peptide 21
Chemical type: Peptide from protein
Sequence: GPSSDPAWERNDPTQQIP
Position: 219-236
Source molecule (GenBank): AAA25359.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Peptide 23
Chemical type: Peptide from protein
Sequence: KLVANNTRLWVYCGNGTP
Position: 237-254
Source molecule (GenBank): AAA25359.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Peptide 24
Chemical type: Peptide from protein
Sequence: WVYCGNGTPNELGGANIP
Position: 246-263
Source molecule (GenBank): AAA25359.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Peptide 25
Chemical type: Peptide from protein
Sequence: NELGGANIPAEFLENFVR
Position: 255-272
Source molecule (GenBank): AAA25359.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Peptide 26
Chemical type: Peptide from protein
Sequence: AEFLENFVRSSNLKFQDA
Position: 264-281
Source molecule (GenBank): AAA25359.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Peptide 27
Chemical type: Peptide from protein
Sequence: SSNLKFQDAYKPAGGHNA
Position: 273-290
Source molecule (GenBank): AAA25359.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Peptide 28
Chemical type: Peptide from protein
Sequence: YKPAGGHNAVFNFPPNGT
Position: 282-299
Source molecule (GenBank): AAA25359.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Peptide 29
Chemical type: Peptide from protein
Sequence: VFNFPPNGTHSWEYWGAQ
Position: 291-308
Source molecule (GenBank): AAA25359.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Peptide 31
Chemical type: Peptide from protein
Sequence: LNAMKGDLQSSLGAG
Position: 309-323
Source molecule (GenBank): AAA25359.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo