Reference: John C Flickinger; Jagmohan Singh; Yanki Yarman; Robert D Carlson; Joshua R Barton; Scott A Waldman; Adam E Snook. "T-Cell Responses to Immunodominant Listeria Epitopes Limit Vaccine-Directed Responses to the Colorectal Cancer Antigen, Guanylyl Cyclase C." Frontiers in immunology (2022).

Epitope: GUCY2C 254-262
Chemical type: Peptide from protein
Sequence: SFYDVKGDL
Position: 254-262
Source molecule (GenBank): AAH99968.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GUCY2C 254-262
Chemical type: Peptide from protein
Sequence: SFYDVKGDL
Position: 254-262
Source molecule (GenBank): AAH99968.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 126.0

Epitope: GUCY2C 254-262 F255Y
Chemical type: Peptide, no natural source
Sequence: SYYDVKGDL
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GUCY2C 254-262 F255Y
Chemical type: Peptide, no natural source
Sequence: SYYDVKGDL
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 498.0

Epitope: LLO 91-90
Chemical type: Peptide from protein
Sequence: GYKDGNEYI
Position: 91-99
Source molecule (GenBank): Q724L1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: LLO 91-90
Chemical type: Peptide from protein
Sequence: GYKDGNEYI
Position: 91-99
Source molecule (GenBank): Q724L1.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 630.0

Epitope: Mpl 84-92
Chemical type: Peptide from protein
Sequence: GYLTDNDEI
Position: 84-92
Source molecule (GenBank): P34025.3
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Mpl 84-92
Chemical type: Peptide from protein
Sequence: GYLTDNDEI
Position: 84-92
Source molecule (GenBank): P34025.3
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 630.0

Epitope: p60 476-484
Chemical type: Peptide from protein
Sequence: KYLVGFGRV
Position: 476-484
Source molecule (GenBank): P21171.2
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p60 476-484
Chemical type: Peptide from protein
Sequence: KYLVGFGRV
Position: 476-484
Source molecule (GenBank): P21171.2
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 630.0

Epitope: p60 217-225
Chemical type: Peptide from protein
Sequence: KYGVSVQDI
Position: 217-225
Source molecule (GenBank): P21171.2
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p60 217-225
Chemical type: Peptide from protein
Sequence: KYGVSVQDI
Position: 217-225
Source molecule (GenBank): P21171.2
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 600.0

Epitope: p60 449-457
Chemical type: Peptide from protein
Sequence: IYVGNGQMI
Position: 449-457
Source molecule (GenBank): P21171.2
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p60 449-457
Chemical type: Peptide from protein
Sequence: IYVGNGQMI
Position: 449-457
Source molecule (GenBank): P21171.2
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 276.0

Epitope: GUCY2C 254-262 V258S
Chemical type: Peptide, no natural source
Sequence: SFYDSKGDL
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo