Reference: Andrew Poole; Vijaykumar Karuppiah; Annabelle Hartt; Jaafar N Haidar; Sylvie Moureau; Tomasz Dobrzycki; Conor Hayes; Christopher Rowley; Jorge Dias; Stephen Harper; Keir Barnbrook; Miriam Hock; Charlotte Coles; Wei Yang; Milos Aleksic; Aimee Bence Lin; Ross Robinson; Joe D Dukes; Nathaniel Liddy; Marc Van der Kamp; Gregory D Plowman; Annelise Vuidepot; David K Cole; Andrew D Whale; Chandramouli Chillakuri. "Therapeutic high affinity T cell receptor targeting a KRAS<sup>G12D</sup> cancer neoantigen." Nature communications (2022).

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive-Low
Quantitative measurement: 3000.0

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive
Quantitative measurement: 3.41

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Negative
Cell type: T cell
Tissue: blood
Culture conditions: Engineered

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive-High
Quantitative measurement: 0.743

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive
Quantitative measurement: 0.00016

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive
Quantitative measurement: 215000.0

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive-Intermediate
Quantitative measurement: 261.0

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Negative

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive
Cell type: T cell
Tissue: blood
Culture conditions: Engineered

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive-Low
Quantitative measurement: 63000.0

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive
Quantitative measurement: 2.64

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive-Low
Quantitative measurement: 2300.0

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive-Low
Quantitative measurement: 460.0

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive-Low
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Negative
Cell type: T cell
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive-Low
Quantitative measurement: 89800.0

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive-High
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive-Low
Quantitative measurement: 425.0

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive
Quantitative measurement: 131000.0

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive-High
Quantitative measurement: 0.0637

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive
Quantitative measurement: 0.00000837

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive-Low
Quantitative measurement: 0.0381

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive
Cell type: T cell CD3+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive-Low
Quantitative measurement: 25800.0

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive
Quantitative measurement: 0.0065

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive
Quantitative measurement: 25000.0

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Negative
Cell type: T cell
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Negative
Cell type: T cell
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 2.64

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 3.41

Epitope: KRAS G12D
Chemical type: Peptide, no natural source
Sequence: VVVGADGVGK
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 1.81

Epitope: KRAS WT
Chemical type: Peptide from protein
Sequence: VVVGAGGVGK
Position: 7-16
Source molecule (GenBank): P01116.1
Assay category: TCell
Result: Positive-Low
Quantitative measurement: 3000.0

Epitope: KRAS WT
Chemical type: Peptide from protein
Sequence: VVVGAGGVGK
Position: 7-16
Source molecule (GenBank): P01116.1
Assay category: TCell
Result: Positive-Low
Quantitative measurement: 25800.0

Epitope: KRAS WT
Chemical type: Peptide from protein
Sequence: VVVGAGGVGK
Position: 7-16
Source molecule (GenBank): P01116.1
Assay category: TCell
Result: Negative
Cell type: T cell
Tissue: blood
Culture conditions: Engineered

Epitope: KRAS WT
Chemical type: Peptide from protein
Sequence: VVVGAGGVGK
Position: 7-16
Source molecule (GenBank): P01116.1
Assay category: TCell
Result: Positive
Quantitative measurement: 2.58

Epitope: KRAS WT
Chemical type: Peptide from protein
Sequence: VVVGAGGVGK
Position: 7-16
Source molecule (GenBank): P01116.1
Assay category: TCell
Result: Negative

Epitope: KRAS WT
Chemical type: Peptide from protein
Sequence: VVVGAGGVGK
Position: 7-16
Source molecule (GenBank): P01116.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: KRAS WT
Chemical type: Peptide from protein
Sequence: VVVGAGGVGK
Position: 7-16
Source molecule (GenBank): P01116.1
Assay category: TCell
Result: Negative
Cell type: T cell
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: KRAS WT
Chemical type: Peptide from protein
Sequence: VVVGAGGVGK
Position: 7-16
Source molecule (GenBank): P01116.1
Assay category: TCell
Result: Positive-Low
Quantitative measurement: 425.0

Epitope: KRAS WT
Chemical type: Peptide from protein
Sequence: VVVGAGGVGK
Position: 7-16
Source molecule (GenBank): P01116.1
Assay category: TCell
Result: Positive-Low
Quantitative measurement: 0.0381

Epitope: KRAS WT
Chemical type: Peptide from protein
Sequence: VVVGAGGVGK
Position: 7-16
Source molecule (GenBank): P01116.1
Assay category: TCell
Result: Positive-Low
Quantitative measurement: 89800.0

Epitope: KRAS WT
Chemical type: Peptide from protein
Sequence: VVVGAGGVGK
Position: 7-16
Source molecule (GenBank): P01116.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 2.46

Epitope: KRAS WT
Chemical type: Peptide from protein
Sequence: VVVGAGGVGK
Position: 7-16
Source molecule (GenBank): P01116.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 2.58

Epitope: RASL10A
Chemical type: Peptide from protein
Sequence: AVLGAPGVGK
Position: 8-17
Source molecule (GenBank): Q92737.1
Assay category: TCell
Result: Positive-Low
Quantitative measurement: 460.0

Epitope: RASL10A
Chemical type: Peptide from protein
Sequence: AVLGAPGVGK
Position: 8-17
Source molecule (GenBank): Q92737.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo