Reference: Rieuwert Hoppes; Rimke Oostvogels; Jolien J Luimstra; Kim Wals; Mireille Toebes; Laura Bies; Reggy Ekkebus; Pramila Rijal; Patrick H N Celie; Julie H Huang; Maarten E Emmelot; Robbert M Spaapen; Henk Lokhorst; Ton N M Schumacher; Tuna Mutis; Boris Rodenko; Huib Ovaa. "Altered peptide ligands revisited: vaccine design through chemically modified HLA-A2-restricted T cell epitopes." Journal of immunology (Baltimore, Md. : 1950) (2014).

Epitope: Mart-1 (26-35)
Chemical type: Peptide from protein
Sequence: EAAGIGILTV
Position: 26-35
Source molecule (GenBank): Q16655.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Mart-1 (26-35)
Chemical type: Peptide from protein
Sequence: EAAGIGILTV
Position: 26-35
Source molecule (GenBank): Q16655.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Mart-1 (26-35)
Chemical type: Peptide from protein
Sequence: EAAGIGILTV
Position: 26-35
Source molecule (GenBank): Q16655.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 14560.0

Epitope: ELA
Chemical type: Peptide, no natural source
Sequence: ELAGIGILTV
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ELA
Chemical type: Peptide, no natural source
Sequence: ELAGIGILTV
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ELA
Chemical type: Peptide, no natural source
Sequence: ELAGIGILTV
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: ELA
Chemical type: Peptide, no natural source
Sequence: ELAGIGILTV
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: ELA
Chemical type: Peptide, no natural source
Sequence: ELAGIGILTV
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 1900.0

Epitope: [am-phg][NVA]AGIGILT[PRG]
Chemical type: Peptide, no natural source
Sequence: GVAGIGILTG
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: [am-phg][NVA]AGIGILT[PRG]
Chemical type: Peptide, no natural source
Sequence: GVAGIGILTG
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: [am-phg][NVA]AGIGILT[PRG]
Chemical type: Peptide, no natural source
Sequence: GVAGIGILTG
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 1.65

Epitope: [am-phg][NVA]AGIGILT[PRG]
Chemical type: Peptide, no natural source
Sequence: GVAGIGILTG
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 510.0

Epitope: Influenza matrix 1 (58-66)
Chemical type: Peptide from protein
Sequence: GILGFVFTL
Position: 58-66
Source molecule (GenBank): ACP44170.1
Assay category: MhcBinding
Result: Positive-High

Epitope: CMV pp65 (495-503)
Chemical type: Peptide from protein
Sequence: NLVPMVATV
Position: 495-503
Source molecule (GenBank): P06725.2
Assay category: MhcBinding
Result: Positive-Intermediate

Epitope: Trp-2 (180-188)
Chemical type: Peptide from protein
Sequence: SVYDFFVWL
Position: 180-188
Source molecule (GenBank): CAA04137.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Trp-2 (180-188)
Chemical type: Peptide from protein
Sequence: SVYDFFVWL
Position: 180-188
Source molecule (GenBank): CAA04137.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 760.0

Epitope: Influenza PB1 (166 - 174)
Chemical type: Peptide from protein
Sequence: FLKDVMESM
Position: 166-174
Source molecule (GenBank): ABO21688.1
Assay category: MhcBinding
Result: Positive

Epitope: Influenza PA (46 - 54)
Chemical type: Peptide from protein
Sequence: FMYSDFHFI
Position: 46-54
Source molecule (GenBank): P03433.2
Assay category: MhcBinding
Result: Positive

Epitope: Influenza PA (225 - 233)
Chemical type: Peptide from protein
Sequence: SLENFRAYV
Position: 225-233
Source molecule (GenBank): NP_775531.1
Assay category: MhcBinding
Result: Positive

Epitope: EBV BMLF - 1 (280 - 288)
Chemical type: Peptide from protein
Sequence: GLCTLVAML
Position: 259-267
Source molecule (GenBank): CAD53409.1
Assay category: MhcBinding
Result: Positive

Epitope: Influenza polymerase PB1 (413 - 421)
Chemical type: Peptide from protein
Sequence: NMLSTVLGV
Position: 413-421
Source molecule (GenBank): P03431.1
Assay category: MhcBinding
Result: Positive

Epitope: melanoma Mage - C2 (HCA587) 248 – 256
Chemical type: Peptide from protein
Sequence: VIWEVLNAV
Position: 248-256
Source molecule (GenBank): Q9UBF1.1
Assay category: MhcBinding
Result: Positive

Epitope: melanoma pmel17/gp100 (256 - 264)
Chemical type: Peptide from protein
Sequence: YLEPGPVTA
Position: 280-288
Source molecule (GenBank): P40967.2
Assay category: MhcBinding
Result: Positive

Epitope: melanoma Mage - A10 (254 - 262)
Chemical type: Peptide from protein
Sequence: GLYDGMEHL
Position: 254-262
Source molecule (GenBank): NP_066386.1
Assay category: MhcBinding
Result: Positive

Epitope: melanoma Trp - 2 (181 - 190)
Chemical type: Peptide from protein
Sequence: VYDFFVWLHY
Position: 181-190
Source molecule (GenBank): CAA04137.1
Assay category: MhcBinding
Result: Positive

Epitope: melanoma MG - 50 (1243 - 1251)
Chemical type: Peptide from protein
Sequence: RLGPTLMCL
Position: 1244-1252
Source molecule (GenBank): AAF06354.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 1780.0

Epitope: melanoma Mage - C2 (336 - 344)
Chemical type: Peptide from protein
Sequence: ALKDVEERV
Position: 336-344
Source molecule (GenBank): Q9UBF1.1
Assay category: MhcBinding
Result: Positive

Epitope: melanoma Mage - C2 (191 - 200)
Chemical type: Peptide from protein
Sequence: LLFGLALIEV
Position: 191-200
Source molecule (GenBank): Q9UBF1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: melanoma Mage - C2 (191 - 200)
Chemical type: Peptide from protein
Sequence: LLFGLALIEV
Position: 191-200
Source molecule (GenBank): Q9UBF1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: melanoma Mage - C2 (191 - 200)
Chemical type: Peptide from protein
Sequence: LLFGLALIEV
Position: 191-200
Source molecule (GenBank): Q9UBF1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: melanoma Mage - C2 (191 - 200)
Chemical type: Peptide from protein
Sequence: LLFGLALIEV
Position: 191-200
Source molecule (GenBank): Q9UBF1.1
Assay category: MhcBinding
Result: Positive

Epitope: [CSME][2-AOC]YDFFVWL
Chemical type: Peptide, no natural source
Sequence: SVYDFFVWL
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: [CSME][2-AOC]YDFFVWL
Chemical type: Peptide, no natural source
Sequence: SVYDFFVWL
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 410.0

Epitope: [Phg][2-AOC]GPTLMCL
Chemical type: Peptide, no natural source
Sequence: GLGPTLMCL
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 400.0

Epitope: [SOME][2-AOC]AGIGILTV
Chemical type: Peptide, no natural source
Sequence: SAAGIGILTV
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: [SOME][2-AOC]AGIGILTV
Chemical type: Peptide, no natural source
Sequence: SAAGIGILTV
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 220.0

Epitope: minor histocompatibility Ag (mHag) UTA2-1
Chemical type: Peptide from protein
Sequence: QLLNSVLTL
Position: 248-256
Source molecule (GenBank): NP_060639.3
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: minor histocompatibility Ag (mHag) UTA2-1
Chemical type: Peptide from protein
Sequence: QLLNSVLTL
Position: 248-256
Source molecule (GenBank): NP_060639.3
Assay category: TCell
Result: Positive
Host: M 
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: minor histocompatibility Ag (mHag) UTA2-1
Chemical type: Peptide from protein
Sequence: QLLNSVLTL
Position: 248-256
Source molecule (GenBank): NP_060639.3
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: minor histocompatibility Ag (mHag) UTA2-1
Chemical type: Peptide from protein
Sequence: QLLNSVLTL
Position: 248-256
Source molecule (GenBank): NP_060639.3
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: minor histocompatibility Ag (mHag) UTA2-1
Chemical type: Peptide from protein
Sequence: QLLNSVLTL
Position: 248-256
Source molecule (GenBank): NP_060639.3
Assay category: TCell
Result: Positive-Low
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: minor histocompatibility Ag (mHag) UTA2-1
Chemical type: Peptide from protein
Sequence: QLLNSVLTL
Position: 248-256
Source molecule (GenBank): NP_060639.3
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: minor histocompatibility Ag (mHag) UTA2-1
Chemical type: Peptide from protein
Sequence: QLLNSVLTL
Position: 248-256
Source molecule (GenBank): NP_060639.3
Assay category: MhcBinding
Result: Positive

Epitope: [SOME]LLNSVLT[NLE]
Chemical type: Peptide, no natural source
Sequence: SLLNSVLTL
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: [SOME]LLNSVLT[NLE]
Chemical type: Peptide, no natural source
Sequence: SLLNSVLTL
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: [SOME]LLNSVLT[NLE]
Chemical type: Peptide, no natural source
Sequence: SLLNSVLTL
Assay category: TCell
Result: Positive
Host: M 
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: [SOME]LLNSVLT[NLE]
Chemical type: Peptide, no natural source
Sequence: SLLNSVLTL
Assay category: MhcBinding
Result: Positive

Epitope: [PHG][BUTALA]LNSVLT[2-AOC]
Chemical type: Peptide, no natural source
Sequence: GALNSVLTL
Assay category: TCell
Result: Positive-High
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: [PHG][BUTALA]LNSVLT[2-AOC]
Chemical type: Peptide, no natural source
Sequence: GALNSVLTL
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: [PHG][BUTALA]LNSVLT[2-AOC]
Chemical type: Peptide, no natural source
Sequence: GALNSVLTL
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: [PHG][BUTALA]LNSVLT[2-AOC]
Chemical type: Peptide, no natural source
Sequence: GALNSVLTL
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: [PHG][BUTALA]LNSVLT[2-AOC]
Chemical type: Peptide, no natural source
Sequence: GALNSVLTL
Assay category: TCell
Result: Positive
Host: M 
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: [PHG][BUTALA]LNSVLT[2-AOC]
Chemical type: Peptide, no natural source
Sequence: GALNSVLTL
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: [PHG][BUTALA]LNSVLT[2-AOC]
Chemical type: Peptide, no natural source
Sequence: GALNSVLTL
Assay category: MhcBinding
Result: Positive

Epitope: [am-phg][NVA]LNSVLT[NLE]
Chemical type: Peptide, no natural source
Sequence: GVLNSVLTL
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: [am-phg][NVA]LNSVLT[NLE]
Chemical type: Peptide, no natural source
Sequence: GVLNSVLTL
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: [am-phg][NVA]LNSVLT[NLE]
Chemical type: Peptide, no natural source
Sequence: GVLNSVLTL
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: [am-phg][NVA]LNSVLT[NLE]
Chemical type: Peptide, no natural source
Sequence: GVLNSVLTL
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: [am-phg][NVA]LNSVLT[NLE]
Chemical type: Peptide, no natural source
Sequence: GVLNSVLTL
Assay category: TCell
Result: Positive
Host: M 
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: [am-phg][NVA]LNSVLT[NLE]
Chemical type: Peptide, no natural source
Sequence: GVLNSVLTL
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: [am-phg][NVA]LNSVLT[NLE]
Chemical type: Peptide, no natural source
Sequence: GVLNSVLTL
Assay category: MhcBinding
Result: Positive