Reference: Jihyun Yang; Eunjin Kim; Jong-Soo Lee; Haryoung Poo. "A Murine CD8<sup>+</sup> T Cell Epitope Identified in the Receptor-Binding Domain of the SARS-CoV-2 Spike Protein." Vaccines (2021).

Epitope: CoV-2 S P2
Chemical type: Peptide from protein
Sequence: IVRFPNITNLCPFGE
Position: 326-340
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P4
Chemical type: Peptide from protein
Sequence: CPFGEVFNATRFASV
Position: 336-350
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P5
Chemical type: Peptide from protein
Sequence: VFNATRFASVYAWNR
Position: 341-355
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P6
Chemical type: Peptide from protein
Sequence: RFASVYAWNRKRISN
Position: 346-360
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P7
Chemical type: Peptide from protein
Sequence: YAWNRKRISNCVADY
Position: 351-365
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P8
Chemical type: Peptide from protein
Sequence: KRISNCVADYSVLYN
Position: 356-370
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P9
Chemical type: Peptide from protein
Sequence: CVADYSVLYNSASFS
Position: 361-375
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P10
Chemical type: Peptide from protein
Sequence: SVLYNSASFSTFKCY
Position: 366-380
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P11
Chemical type: Peptide from protein
Sequence: SASFSTFKCYGVSPT
Position: 371-385
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P12
Chemical type: Peptide from protein
Sequence: TFKCYGVSPTKLNDL
Position: 376-390
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P13
Chemical type: Peptide from protein
Sequence: GVSPTKLNDLCFTNV
Position: 381-395
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P14
Chemical type: Peptide from protein
Sequence: KLNDLCFTNVYADSF
Position: 386-400
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P15
Chemical type: Peptide from protein
Sequence: CFTNVYADSFVIRGD
Position: 391-405
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P16
Chemical type: Peptide from protein
Sequence: YADSFVIRGDEVRQI
Position: 396-410
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P17
Chemical type: Peptide from protein
Sequence: VIRGDEVRQIAPGQT
Position: 401-415
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P18
Chemical type: Peptide from protein
Sequence: EVRQIAPGQTGKIAD
Position: 406-420
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P19
Chemical type: Peptide from protein
Sequence: APGQTGKIADYNYKL
Position: 411-425
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P20
Chemical type: Peptide from protein
Sequence: TKIADYNYKLPDDFT
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P21
Chemical type: Peptide from protein
Sequence: YNYKLPDDFTGCVIA
Position: 421-435
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P22
Chemical type: Peptide from protein
Sequence: PDDFTGCVIAWNSNN
Position: 426-440
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P24
Chemical type: Peptide from protein
Sequence: WNSNNLDSKVGGNYN
Position: 436-450
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P25
Chemical type: Peptide from protein
Sequence: LDSKVGGNYNYLYRL
Position: 441-455
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P26
Chemical type: Peptide from protein
Sequence: GGNYNYLYRLFRKSN
Position: 446-460
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P27
Chemical type: Peptide from protein
Sequence: YLYRLFRKSNLKPFE
Position: 451-465
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P29
Chemical type: Peptide from protein
Sequence: LKPFERDISTEIYQA
Position: 461-475
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P30
Chemical type: Peptide from protein
Sequence: RDISTEIYQAGSTPC
Position: 466-480
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P32
Chemical type: Peptide from protein
Sequence: GSTPCNGVEGFNCYF
Position: 476-490
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P33
Chemical type: Peptide from protein
Sequence: NGVEGFNCYFPLQSY
Position: 481-495
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P34
Chemical type: Peptide from protein
Sequence: FNCYFPLQSYGFQPT
Position: 486-500
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P35
Chemical type: Peptide from protein
Sequence: PLQSYGFQPTNGVGY
Position: 491-505
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P36
Chemical type: Peptide from protein
Sequence: GFQPTNGVGYQPYRV
Position: 496-510
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P37
Chemical type: Peptide from protein
Sequence: NGVGYQPYRVVVLSF
Position: 501-515
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P38
Chemical type: Peptide from protein
Sequence: QPYRVVVLSFELLHA
Position: 506-520
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P39
Chemical type: Peptide from protein
Sequence: VVLSFELLHAPATVC
Position: 511-525
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P40
Chemical type: Peptide from protein
Sequence: ELLHAPATVCGPKKS
Position: 516-530
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S P42
Chemical type: Peptide from protein
Sequence: CGKKSTNLVKNKCVN
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 15-10-5
Chemical type: Peptide from protein
Sequence: VYADSFVIRG
Position: 395-404
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 15-10-5
Chemical type: Peptide from protein
Sequence: VYADSFVIRG
Position: 395-404
Source molecule (GenBank): YP_009724390.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo