Reference: Stefania Senger; Francesco Maurano; Maria F Mazzeo; Marcello Gaita; Olga Fierro; Chella S David; Riccardo Troncone; Salvatore Auricchio; Rosa A Siciliano; Mauro Rossi. "Identification of immunodominant epitopes of alpha-gliadin in HLA-DQ8 transgenic mice following oral immunization." Journal of immunology (Baltimore, Md. : 1950) (2005).

Epitope: p1 (1-20)
Chemical type: Peptide from protein
Sequence: VRVPVPQLQPQNPSQQQPQE
Position: 21-40
Source molecule (GenBank): CAA10257.1
Assay category: TCell
Result: Negative
Host:  8-14 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p2 (10-29)
Chemical type: Peptide from protein
Sequence: PQNPSQQQPQEQVPLVQQQQ
Position: 30-49
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Negative
Host:  8-14 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p3 (20-39)
Chemical type: Peptide from protein
Sequence: EQVPLVQQQQFLGQQQPFPP
Position: 40-59
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Negative
Host:  8-14 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p4 (30-49)
Chemical type: Peptide from protein
Sequence: FLGQQQPFPPQQPYPQPQPF
Position: 50-69
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Negative
Host:  8-14 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p5 (40-59)
Chemical type: Peptide from protein
Sequence: QQPYPQPQPFPSQQPYLQLQ
Position: 60-79
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Negative
Host:  8-14 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p8 (70-89)
Chemical type: Peptide from protein
Sequence: QPQPFRPQQPYPQPQPQYSQ
Position: 90-109
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Negative
Host:  8-14 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p9 (80-99)
Chemical type: Peptide from protein
Sequence: YPQPQPQYSQPQQPISQQQQ
Position: 100-119
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Negative
Host:  8-14 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p10 (90-109)
Chemical type: Peptide from protein
Sequence: PQQPISQQQQQQQQQQQQQQ
Position: 110-129
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Negative
Host:  8-14 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p11 (100-119)
Chemical type: Peptide from protein
Sequence: QQQQQQQQQQQQQQQQILQQ
Position: 120-139
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Negative
Host:  8-14 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p12 (110-129)
Chemical type: Peptide from protein
Sequence: QQQQQQILQQILQQQLIPCM
Position: 130-149
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Negative
Host:  8-14 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p13 (120-139)
Chemical type: Peptide from protein
Sequence: ILQQQLIPCMDVVLQQHNIA
Position: 140-159
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Positive
Host:  8-14 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: p13 (120-139)
Chemical type: Peptide from protein
Sequence: ILQQQLIPCMDVVLQQHNIA
Position: 140-159
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Positive
Host:  8-14 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p13 (120-139)
Chemical type: Peptide from protein
Sequence: ILQQQLIPCMDVVLQQHNIA
Position: 140-159
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Negative
Host:  8-14 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: p13 (120-139)
Chemical type: Peptide from protein
Sequence: ILQQQLIPCMDVVLQQHNIA
Position: 140-159
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Positive
Host:  8-14 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Short Term Restimulated

Epitope: p13 (120-139)
Chemical type: Peptide from protein
Sequence: ILQQQLIPCMDVVLQQHNIA
Position: 140-159
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Negative
Host:  8-14 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: p14 (130-149)
Chemical type: Peptide from protein
Sequence: DVVLQQHNIAHGRSQVLQQS
Position: 150-169
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Negative
Host:  8-14 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p15 (141-160)
Chemical type: Peptide from protein
Sequence: GRSQVLQQSTYQLLQELCCQ
Position: 161-180
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Negative
Host:  8-14 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p16 (150-169)
Chemical type: Peptide from protein
Sequence: TYQLLQELCCQHLWQIPEQS
Position: 170-189
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Negative
Host:  8-14 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p17 (160-179)
Chemical type: Peptide from protein
Sequence: QHLWQIPEQSQCQAIHKVVH
Position: 180-199
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Negative
Host:  8-14 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p18 (170-189)
Chemical type: Peptide from protein
Sequence: QCQAIHKVVHAHLHQQQKQ
Assay category: TCell
Result: Negative
Host:  8-14 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p19 (180-199)
Chemical type: Peptide from protein
Sequence: AHLHQQQKQQQQPSSQVSF
Assay category: TCell
Result: Negative
Host:  8-14 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p20 (190-209)
Chemical type: Peptide from protein
Sequence: QQQPSSQVSFQQPLQQYPLG
Position: 210-229
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Negative
Host:  8-14 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p21 (200-219)
Chemical type: Peptide from protein
Sequence: QQPLQQYPLGQGSFRPSQQN
Position: 220-239
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Negative
Host:  8-14 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p22 (210-229)
Chemical type: Peptide from protein
Sequence: QGSFRPSQQNPQAQGSVQPQ
Position: 230-249
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Negative
Host:  8-14 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p23 (220-239)
Chemical type: Peptide from protein
Sequence: PQAQGSVQPQQLPQFEEIRN
Position: 240-259
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Negative
Host:  8-14 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: p23 (220-239)
Chemical type: Peptide from protein
Sequence: PQAQGSVQPQQLPQFEEIRN
Position: 240-259
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Negative
Host:  8-14 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: p23 (220-239)
Chemical type: Peptide from protein
Sequence: PQAQGSVQPQQLPQFEEIRN
Position: 240-259
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Positive
Host:  8-14 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: p23 (220-239)
Chemical type: Peptide from protein
Sequence: PQAQGSVQPQQLPQFEEIRN
Position: 240-259
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Positive
Host:  8-14 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p24 (230-249)
Chemical type: Peptide from protein
Sequence: QLPQFEEIRNLALQTLPAMC
Position: 250-269
Source molecule (GenBank): CAA10257.1
Assay category: TCell
Result: Negative
Host:  8-14 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: p26 (250-268)
Chemical type: Peptide from protein
Sequence: NVYIPPYCTITPFGIFGTN
Position: 270-288
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Negative
Host:  8-14 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: giladin 123-132
Chemical type: Peptide from protein
Sequence: QLIPCMDVVL
Position: 144-153
Source molecule (GenBank): Q9ZP09.1
Assay category: TCell
Result: Positive
Host:  8-14 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo