Reference: Phuong-Cac T Nguyen; Kenneth B Lewis; Ruth A Ettinger; Jason T Schuman; Jasper C Lin; John F Healey; Shannon L Meeks; Pete Lollar; Kathleen P Pratt. "High-resolution mapping of epitopes on the C2 domain of factor VIII by analysis of point mutants using surface plasmon resonance." Blood (2014).

Epitope: mAb ESH4 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: E2200, D2206, T2221, S2225, K2226, H2230, L2231, Q2232
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.37
Host: F 12 weeks

Epitope: mAb ESH4 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: E2200, D2206, T2221, S2225, K2226, H2230, L2231, Q2232
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 3800000.0
Host: F 12 weeks

Epitope: mAb ESH4 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: E2200, D2206, T2221, S2225, K2226, H2230, L2231, Q2232
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.0014
Host: F 12 weeks

Epitope: mAb 3E6 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: D2206, K2226, H2230, L2231, Q2232
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 6600000.0
Host:  9-12 weeks

Epitope: mAb 3E6 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: D2206, K2226, H2230, L2231, Q2232
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.0027
Host:  9-12 weeks

Epitope: mAb 3E6 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: D2206, K2226, H2230, L2231, Q2232
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.41
Host:  9-12 weeks

Epitope: mAb I54 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: E2200, D2206, S2225, K2226, H2230, L2231, Q2232
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.0000075
Host:  9-12 weeks

Epitope: mAb I54 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: E2200, D2206, S2225, K2226, H2230, L2231, Q2232
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.0079
Host:  9-12 weeks

Epitope: mAb I54 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: E2200, D2206, S2225, K2226, H2230, L2231, Q2232
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 2800000.0
Host:  9-12 weeks

Epitope: mAb I109 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: F2215, N2217, F2219, T2221, R2239, S2269, L2270, L2271, T2272, S2273, H2334
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.018
Host:  9-12 weeks

Epitope: mAb I109 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: F2215, N2217, F2219, T2221, R2239, S2269, L2270, L2271, T2272, S2273, H2334
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 2700000.0
Host:  9-12 weeks

Epitope: mAb I109 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: F2215, N2217, F2219, T2221, R2239, S2269, L2270, L2271, T2272, S2273, H2334
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.000049
Host:  9-12 weeks

Epitope: mAb 1B5 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: F2215, T2216, N2217, F2219, T2221, R2239, Q2241, N2244, E2247, L2271, S2273, H2334, Q2335
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.000028
Host:  9-12 weeks

Epitope: mAb 1B5 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: F2215, T2216, N2217, F2219, T2221, R2239, Q2241, N2244, E2247, L2271, S2273, H2334, Q2335
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.024
Host:  9-12 weeks

Epitope: mAb 1B5 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: F2215, T2216, N2217, F2219, T2221, R2239, Q2241, N2244, E2247, L2271, S2273, H2334, Q2335
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 1200000.0
Host:  9-12 weeks

Epitope: mAb 3D12 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: Y2214, F2215, N2217, M2218, F2219, T2221, R2239, N2243, N2244, E2247, K2268, S2269, L2270, L2271, T2272, S2273, H2328, Q2335
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.0059
Host:  9-12 weeks

Epitope: mAb 3D12 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: Y2214, F2215, N2217, M2218, F2219, T2221, R2239, N2243, N2244, E2247, K2268, S2269, L2270, L2271, T2272, S2273, H2328, Q2335
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.000058
Host:  9-12 weeks

Epitope: mAb 3D12 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: Y2214, F2215, N2217, M2218, F2219, T2221, R2239, N2243, N2244, E2247, K2268, S2269, L2270, L2271, T2272, S2273, H2328, Q2335
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 9800000.0
Host:  9-12 weeks

Epitope: mAb 3G6 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: N2244, E2247, Q2289, L2292, R2326, H2328
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.000033
Host:  9-12 weeks

Epitope: mAb 3G6 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: N2244, E2247, Q2289, L2292, R2326, H2328
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.02
Host:  9-12 weeks

Epitope: mAb 3G6 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: N2244, E2247, Q2289, L2292, R2326, H2328
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 1700000.0
Host:  9-12 weeks

Epitope: mAb 2-77 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: N2244, E2247, H2288, L2292, V2299, R2326, H2328
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 1100000.0
Host:  9-12 weeks

Epitope: mAb 2-77 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: N2244, E2247, H2288, L2292, V2299, R2326, H2328
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.00014
Host:  9-12 weeks

Epitope: mAb 2-77 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: N2244, E2247, H2288, L2292, V2299, R2326, H2328
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.13
Host:  9-12 weeks

Epitope: mAb ESH8 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: T2291, L2292, V2299, V2301, H2328, Q2330
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 1900000.0
Host: F 12 weeks

Epitope: mAb ESH8 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: T2291, L2292, V2299, V2301, H2328, Q2330
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.095
Host: F 12 weeks

Epitope: mAb ESH8 epitope
Chemical type: Discontinuous protein residues
Discontinuous residues: T2291, L2292, V2299, V2301, H2328, Q2330
Source molecule (GenBank): P00451.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.00018
Host: F 12 weeks