Reference: George Elias; Pieter Meysman; Esther Bartholomeus; Nicolas De Neuter; Nina Keersmaekers; Arvid Suls; Hilde Jansens; Aisha Souquette; Hans De Reu; Marie-Paule Emonds; Evelien Smits; Eva Lion; Paul G Thomas; Geert Mortier; Pierre Van Damme; Philippe Beutels; Kris Laukens; Viggo Van Tendeloo; Benson Ogunjimi. "Preexisting memory CD4 T cells in naïve individuals confer robust immunity upon hepatitis B vaccination." eLife (2022).

Epitope: HBsAg 1
Chemical type: Peptide from protein
Sequence: MENITSGFLGPLLVL
Position: 164-178
Source molecule (GenBank): P03138.3
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 2
Chemical type: Peptide from protein
Sequence: TSGFLGPLLVLQAGF
Position: 179-193
Source molecule (GenBank): P31868.2
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 3
Chemical type: Peptide from protein
Sequence: LGPLLVLQAGFFLLT
Position: 183-197
Source molecule (GenBank): P31868.2
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 4
Chemical type: Peptide from protein
Sequence: LVLQAGFFLLTRILT
Position: 187-201
Source molecule (GenBank): P31868.2
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 5
Chemical type: Peptide from protein
Sequence: AGFFLLTRILTIPQS
Position: 191-205
Source molecule (GenBank): P31868.2
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 6
Chemical type: Peptide from protein
Sequence: LLTRILTIPQSLDSW
Position: 195-209
Source molecule (GenBank): P31868.2
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 7
Chemical type: Peptide from protein
Sequence: ILTIPQSLDSWWTSL
Position: 199-213
Source molecule (GenBank): P31868.2
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 8
Chemical type: Peptide from protein
Sequence: PQSLDSWWTSLNFLG
Position: 203-217
Source molecule (GenBank): P31868.2
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 9
Chemical type: Peptide from protein
Sequence: DSWWTSLNFLGGSPV
Position: 196-210
Source molecule (GenBank): Q91C35.1
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 10
Chemical type: Peptide from protein
Sequence: TSLNFLGGSPVCLGQ
Position: 200-214
Source molecule (GenBank): Q91C35.1
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 11
Chemical type: Peptide from protein
Sequence: FLGGSPVCLGQNSQS
Position: 215-229
Source molecule (GenBank): P31873.2
Assay category: TCell
Result: Negative
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 12
Chemical type: Peptide from protein
Sequence: SPVCLGQNSQSPTSN
Position: 219-233
Source molecule (GenBank): P31873.2
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 13
Chemical type: Peptide from protein
Sequence: LGQNSQSPTSNHSPT
Position: 212-226
Source molecule (GenBank): P03138.3
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 15
Chemical type: Peptide from protein
Sequence: TSNHSPTSCPPICPG
Position: 231-245
Source molecule (GenBank): P31868.2
Assay category: TCell
Result: Negative
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 16
Chemical type: Peptide from protein
Sequence: SPTSCPPICPGYRWM
Position: 235-249
Source molecule (GenBank): P31868.2
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 17
Chemical type: Peptide from protein
Sequence: CPPICPGYRWMCLRR
Position: 239-253
Source molecule (GenBank): P31868.2
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 18
Chemical type: Peptide from protein
Sequence: CPGYRWMCLRRFIIF
Position: 243-257
Source molecule (GenBank): P31868.2
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 19
Chemical type: Peptide from protein
Sequence: RWMCLRRFIIFLFIL
Position: 247-261
Source molecule (GenBank): P31868.2
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 21
Chemical type: Peptide from protein
Sequence: IIFLFILLLCLIFLL
Position: 255-269
Source molecule (GenBank): P31868.2
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 22
Chemical type: Peptide from protein
Sequence: FILLLCLIFLLVLLD
Position: 259-273
Source molecule (GenBank): P31868.2
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 23
Chemical type: Peptide from protein
Sequence: LCLIFLLVLLDYQGM
Position: 263-277
Source molecule (GenBank): P31868.2
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 24
Chemical type: Peptide from protein
Sequence: FLLVLLDYQGMLPVC
Position: 267-281
Source molecule (GenBank): P31868.2
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 25
Chemical type: Peptide from protein
Sequence: LLDYQGMLPVCPLIP
Position: 260-274
Source molecule (GenBank): P03138.3
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 26
Chemical type: Peptide from protein
Sequence: QGMLPVCPLIPGSTT
Position: 275-289
Source molecule (GenBank): Q4R1R8.1
Assay category: TCell
Result: Negative
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 27
Chemical type: Peptide from protein
Sequence: PVCPLIPGSTTTNTG
Position: 279-293
Source molecule (GenBank): A0A076Q219.1
Assay category: TCell
Result: Negative
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 28
Chemical type: Peptide from protein
Sequence: LIPGSTTTNTGPCKT
Position: 283-297
Source molecule (GenBank): A0A076Q219.1
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 29
Chemical type: Peptide from protein
Sequence: STTTNTGPCKTCTTP
Position: 287-301
Source molecule (GenBank): A0A076Q219.1
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 30
Chemical type: Peptide from protein
Sequence: NTGPCKTCTTPAQGN
Position: 291-305
Source molecule (GenBank): A0A076Q219.1
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 31
Chemical type: Peptide from protein
Sequence: CKTCTTPAQGNSMFP
Position: 284-298
Source molecule (GenBank): Q91C35.1
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 32
Chemical type: Peptide from protein
Sequence: TTPAQGNSMFPSCCC
Position: 299-313
Source molecule (GenBank): Q4R1R8.1
Assay category: TCell
Result: Negative
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 33
Chemical type: Peptide from protein
Sequence: QGNSMFPSCCCTKPT
Position: 303-317
Source molecule (GenBank): Q4R1R8.1
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 34
Chemical type: Peptide from protein
Sequence: MFPSCCCTKPTDGNC
Position: 307-321
Source molecule (GenBank): Q4R1R8.1
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 35
Chemical type: Peptide from protein
Sequence: CCCTKPTDGNCTCIP
Position: 311-325
Source molecule (GenBank): Q4R1R8.1
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 36
Chemical type: Peptide from protein
Sequence: KPTDGNCTCIPIPSS
Position: 315-329
Source molecule (GenBank): Q4R1R8.1
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 37
Chemical type: Peptide from protein
Sequence: GNCTCIPIPSSWAFA
Position: 319-333
Source molecule (GenBank): P31868.2
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 38
Chemical type: Peptide from protein
Sequence: CIPIPSSWAFAKYLW
Position: 323-337
Source molecule (GenBank): Q4R1R8.1
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 39
Chemical type: Peptide from protein
Sequence: PSSWAFAKYLWEWAS
Position: 327-341
Source molecule (GenBank): Q4R1R8.1
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 40
Chemical type: Peptide from protein
Sequence: AFAKYLWEWASVRFS
Position: 331-345
Source molecule (GenBank): Q4R1R8.1
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 42
Chemical type: Peptide from protein
Sequence: WASVRFSWLSLLVPF
Position: 339-353
Source molecule (GenBank): P31868.2
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 43
Chemical type: Peptide from protein
Sequence: RFSWLSLLVPFVQWF
Position: 343-357
Source molecule (GenBank): P31868.2
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 44
Chemical type: Peptide from protein
Sequence: LSLLVPFVQWFVGLS
Position: 336-350
Source molecule (GenBank): P03138.3
Assay category: TCell
Result: Negative
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 45
Chemical type: Peptide from protein
Sequence: VPFVQWFVGLSPTVW
Position: 340-354
Source molecule (GenBank): P03138.3
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 46
Chemical type: Peptide from protein
Sequence: QWFVGLSPTVWLSAI
Position: 354-368
Source molecule (GenBank): Q9IBI3.1
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 47
Chemical type: Peptide from protein
Sequence: GLSPTVWLSAIWMMW
Position: 358-372
Source molecule (GenBank): Q9IBI3.1
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 48
Chemical type: Peptide from protein
Sequence: TVWLSAIWMMWYWGP
Position: 362-376
Source molecule (GenBank): Q9IBI3.1
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 49
Chemical type: Peptide from protein
Sequence: SAIWMMWYWGPSLYS
Position: 367-381
Source molecule (GenBank): P03141.3
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 50
Chemical type: Peptide from protein
Sequence: MMWYWGPSLYSIVSP
Position: 371-385
Source molecule (GenBank): P03141.3
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 51
Chemical type: Peptide from protein
Sequence: WGPSLYSIVSPFIPL
Position: 375-389
Source molecule (GenBank): P03141.3
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 52
Chemical type: Peptide from protein
Sequence: LYSIVSPFIPLLPIF
Position: 379-393
Source molecule (GenBank): P03141.3
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HBsAg 53
Chemical type: Peptide from protein
Sequence: VSPFIPLLPIFFCLW
Position: 372-386
Source molecule (GenBank): Q91C35.1
Assay category: TCell
Result: Positive
Host:  21-50 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo