Reference: Tracy J Yuen; Inge E A Flesch; Natasha A Hollett; Bianca M Dobson; Tiffany A Russell; Aude M Fahrer; David C Tscharke. "Analysis of A47, an immunoprevalent protein of vaccinia virus, leads to a reevaluation of the total antiviral CD8+ T cell response." Journal of virology (2010).

Epitope: A47<sub>138-146</sub>
Chemical type: Peptide from protein
Sequence: AAFEFINSL
Position: 138-146
Source molecule (GenBank): P26673.2
Assay category: TCell
Result: Negative
Host: F >8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A47<sub>138-146</sub>
Chemical type: Peptide from protein
Sequence: AAFEFINSL
Position: 138-146
Source molecule (GenBank): P26673.2
Assay category: TCell
Result: Positive
Host: F >8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A47<sub>93-101</sub>
Chemical type: Peptide from protein
Sequence: TMMINPFMI
Position: 93-101
Source molecule (GenBank): P26673.2
Assay category: TCell
Result: Positive-Low
Host: F >8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A47<sub>171-180</sub>
Chemical type: Peptide from protein
Sequence: YAHINALEYI
Position: 171-180
Source molecule (GenBank): P26673.2
Assay category: TCell
Result: Positive-Low
Host: F >8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A47<sub>171-180</sub>
Chemical type: Peptide from protein
Sequence: YAHINALEYI
Position: 171-180
Source molecule (GenBank): P26673.2
Assay category: TCell
Result: Positive
Host: F >8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B8<sub>20</sub>
Chemical type: Peptide from protein
Sequence: TSYKFESV
Position: 20-27
Source molecule (GenBank): P24770.1
Assay category: TCell
Result: Positive
Host: F >8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B8<sub>20</sub>
Chemical type: Peptide from protein
Sequence: TSYKFESV
Position: 20-27
Source molecule (GenBank): P24770.1
Assay category: TCell
Result: Positive
Host: F >8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A8<sub>189</sub>
Chemical type: Peptide from protein
Sequence: ITYRFYLI
Position: 189-196
Source molecule (GenBank): P68720.1
Assay category: TCell
Result: Positive-Low
Host: F >8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A8<sub>189</sub>
Chemical type: Peptide from protein
Sequence: ITYRFYLI
Position: 189-196
Source molecule (GenBank): P68720.1
Assay category: TCell
Result: Positive
Host: F >8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A23<sub>297</sub>
Chemical type: Peptide from protein
Sequence: IGMFNLTFI
Position: 297-305
Source molecule (GenBank): Q80HV2.1
Assay category: TCell
Result: Positive-Low
Host: F >8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A23<sub>297</sub>
Chemical type: Peptide from protein
Sequence: IGMFNLTFI
Position: 297-305
Source molecule (GenBank): Q80HV2.1
Assay category: TCell
Result: Positive
Host: F >8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: K3<sub>6</sub>
Chemical type: Peptide from protein
Sequence: YSLPNAGDVI
Position: 6-15
Source molecule (GenBank): P18378.1
Assay category: TCell
Result: Positive-Low
Host: F >8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: K3<sub>6</sub>
Chemical type: Peptide from protein
Sequence: YSLPNAGDVI
Position: 6-15
Source molecule (GenBank): P18378.1
Assay category: TCell
Result: Positive
Host: F >8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A3<sub>270</sub>
Chemical type: Peptide from protein
Sequence: KSYNYMLL
Position: 270-277
Source molecule (GenBank): P06440.1
Assay category: TCell
Result: Positive
Host: F >8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A3<sub>270</sub>
Chemical type: Peptide from protein
Sequence: KSYNYMLL
Position: 270-277
Source molecule (GenBank): P06440.1
Assay category: TCell
Result: Positive
Host: F >8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: L2<sub>53</sub>
Chemical type: Peptide from protein
Sequence: VIYIFTVRL
Position: 53-61
Source molecule (GenBank): P07613.1
Assay category: TCell
Result: Positive
Host: F >8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: L2<sub>53</sub>
Chemical type: Peptide from protein
Sequence: VIYIFTVRL
Position: 53-61
Source molecule (GenBank): P07613.1
Assay category: TCell
Result: Positive
Host: F >8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A8<sub>70</sub>
Chemical type: Peptide from protein
Sequence: IHYLFRCV
Position: 70-77
Source molecule (GenBank): P68720.1
Assay category: TCell
Result: Negative
Host: F >8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A8<sub>70</sub>
Chemical type: Peptide from protein
Sequence: IHYLFRCV
Position: 70-77
Source molecule (GenBank): P68720.1
Assay category: TCell
Result: Positive
Host: F >8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A42<sub>88</sub>
Chemical type: Peptide from protein
Sequence: YAPVSPIVI
Position: 88-96
Source molecule (GenBank): Q76ZN5.1
Assay category: TCell
Result: Negative
Host: F >8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A42<sub>88</sub>
Chemical type: Peptide from protein
Sequence: YAPVSPIVI
Position: 88-96
Source molecule (GenBank): Q76ZN5.1
Assay category: TCell
Result: Positive-Low
Host: F >8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A19<sub>47</sub>
Chemical type: Peptide from protein
Sequence: VSLDYINTM
Position: 47-55
Source molecule (GenBank): P68714.1
Assay category: TCell
Result: Positive
Host: F >8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A19<sub>47</sub>
Chemical type: Peptide from protein
Sequence: VSLDYINTM
Position: 47-55
Source molecule (GenBank): P68714.1
Assay category: TCell
Result: Positive-Low
Host: F >8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo