Reference: Lena Immisch; George Papafotiou; Oliver Popp; Philipp Mertins; Thomas Blankenstein; Gerald Willimsky. "H3.3K27M mutation is not a suitable target for immunotherapy in HLA-A2<sup>+</sup> patients with diffuse midline glioma." Journal for immunotherapy of cancer (2022).

Epitope: H3.3K27M 26-35
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Engineered

Epitope: H3.3K27M 26-35
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3K27M 26-35
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3K27M 26-35
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Engineered

Epitope: H3.3K27M 26-35
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3K27M 26-35
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3K27M 26-35
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: H3.3K27M 26-35
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3K27M 26-35
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Engineered

Epitope: H3.3K27M 26-35
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3K27M 26-35
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Engineered

Epitope: H3.3K27M 26-35
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Engineered

Epitope: H3.3K27M 26-35
Chemical type: Peptide, no natural source
Sequence: RMSAPSTGGV
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Engineered

Epitope: H3.3 26-35 WT
Chemical type: Peptide from protein
Sequence: RKSAPSTGGV
Position: 27-36
Source molecule (GenBank): P84243.2
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 26-35 WT
Chemical type: Peptide from protein
Sequence: RKSAPSTGGV
Position: 27-36
Source molecule (GenBank): P84243.2
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 26-35 WT
Chemical type: Peptide from protein
Sequence: RKSAPSTGGV
Position: 27-36
Source molecule (GenBank): P84243.2
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 26-35 WT
Chemical type: Peptide from protein
Sequence: RKSAPSTGGV
Position: 27-36
Source molecule (GenBank): P84243.2
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 26-35 WT
Chemical type: Peptide from protein
Sequence: RKSAPSTGGV
Position: 27-36
Source molecule (GenBank): P84243.2
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Engineered

Epitope: H3.3 26-35 WT
Chemical type: Peptide from protein
Sequence: RKSAPSTGGV
Position: 27-36
Source molecule (GenBank): P84243.2
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Engineered