Reference: Satoshi Nagamata; Taiki Aoshi; Akiko Kawabata; Yoshiaki Yamagishi; Mitsuhiro Nishimura; Soichiro Kuwabara; Kouki Murakami; Hideto Yamada; Yasuko Mori. "Identification of CD4 and H-2K<sup>d</sup>-restricted cytotoxic T lymphocyte epitopes on the human herpesvirus 6B glycoprotein Q1 protein." Scientific reports (2019).

Epitope: P1
Chemical type: Peptide from protein
Sequence: VYRDAGTVESTPPPDDEDNYTAKYY
Position: 26-50
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P2
Chemical type: Peptide from protein
Sequence: DEDNYTAKYYDDSIYFNIYD
Position: 41-60
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P3
Chemical type: Peptide from protein
Sequence: DDSIYFNIYDGTNPTPRRRT
Position: 51-70
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P4
Chemical type: Peptide from protein
Sequence: GTNPTPRRRTLPEIISKFST
Position: 61-80
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P5
Chemical type: Peptide from protein
Sequence: LPEIISKFSTSEMSRLGGLK
Position: 71-90
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P7
Chemical type: Peptide from protein
Sequence: VFVPVDYTPTTTLEDIEDLL
Position: 91-110
Source molecule (GenBank): APO37184.1
Assay category: TCell
Result: Positive-Low
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P8
Chemical type: Peptide from protein
Sequence: TTLEDIEDLLNYAICDDNSC
Position: 101-120
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Positive-Low
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P9
Chemical type: Peptide from protein
Sequence: NYAICDDNSCGCLIETEARI
Position: 111-130
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P10
Chemical type: Peptide from protein
Sequence: GCLIETEARIMFGDIIICVP
Position: 121-140
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P11
Chemical type: Peptide from protein
Sequence: MFGDIIICVPLSAESRGVRN
Position: 131-150
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Positive-Low
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P11
Chemical type: Peptide from protein
Sequence: MFGDIIICVPLSAESRGVRN
Position: 131-150
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P11
Chemical type: Peptide from protein
Sequence: MFGDIIICVPLSAESRGVRN
Position: 131-150
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P12
Chemical type: Peptide from protein
Sequence: LSAESRGVRNLKNRLMPMGL
Position: 141-160
Source molecule (GenBank): APO37184.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P13
Chemical type: Peptide from protein
Sequence: LKNRLMPMGLSQILSSGLGL
Position: 151-170
Source molecule (GenBank): APO37184.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P14
Chemical type: Peptide from protein
Sequence: SQILSSGLGLHFSLLYGAFG
Position: 161-180
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P16
Chemical type: Peptide from protein
Sequence: SNYNSLAYMERLKPLTAMTA
Position: 181-200
Source molecule (GenBank): APO37184.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P16
Chemical type: Peptide from protein
Sequence: SNYNSLAYMERLKPLTAMTA
Position: 181-200
Source molecule (GenBank): APO37184.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P16
Chemical type: Peptide from protein
Sequence: SNYNSLAYMERLKPLTAMTA
Position: 181-200
Source molecule (GenBank): APO37184.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P17
Chemical type: Peptide from protein
Sequence: RLKPLTAMTAIAFCPMTSKL
Position: 191-210
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Positive-Low
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P17
Chemical type: Peptide from protein
Sequence: RLKPLTAMTAIAFCPMTSKL
Position: 191-210
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P18
Chemical type: Peptide from protein
Sequence: IAFCPMTSKLELRQNYRLEK
Position: 201-220
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Positive-Low
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P18
Chemical type: Peptide from protein
Sequence: IAFCPMTSKLELRQNYRLEK
Position: 201-220
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P19
Chemical type: Peptide from protein
Sequence: ELRQNYRLEKARCELIVNIE
Position: 211-230
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P20
Chemical type: Peptide from protein
Sequence: ARCELIVNIELLKIQNHGGQ
Position: 221-240
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P21
Chemical type: Peptide from protein
Sequence: LLKIQNHGGQTIKTLTSFAI
Position: 231-250
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P22
Chemical type: Peptide from protein
Sequence: TIKTLTSFAIVRKDNDGQDW
Position: 241-260
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P23
Chemical type: Peptide from protein
Sequence: VRKDNDGQDWETCTRFASVS
Position: 251-270
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P24
Chemical type: Peptide from protein
Sequence: ETCTRFASVSIEDILKSKPA
Position: 261-280
Source molecule (GenBank): APO37184.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P25
Chemical type: Peptide from protein
Sequence: IEDILKSKPAANGTCCPPRD
Position: 271-290
Source molecule (GenBank): APO37184.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P26
Chemical type: Peptide from protein
Sequence: ANGTCCPPRDVHHDRPTLQS
Position: 281-300
Source molecule (GenBank): APO37184.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P27
Chemical type: Peptide from protein
Sequence: VHHDRPTLQSSNSWTRTEYF
Position: 291-310
Source molecule (GenBank): APO37184.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P28
Chemical type: Peptide from protein
Sequence: SNSWTRTEYFEPWQDVVDAY
Position: 301-320
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P29
Chemical type: Peptide from protein
Sequence: EPWQDVVDAYVPINDNHCPN
Position: 311-330
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P30
Chemical type: Peptide from protein
Sequence: VPINDNHCPNDSYVVFQTLQ
Position: 321-340
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P32
Chemical type: Peptide from protein
Sequence: GHEWCSRLNKNDTKNYLSSV
Position: 341-360
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P33
Chemical type: Peptide from protein
Sequence: NDTKNYLSSVLAFKNALYET
Position: 351-370
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Positive-Low
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P34
Chemical type: Peptide from protein
Sequence: LAFKNALYETEELMETIGMR
Position: 361-380
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P35
Chemical type: Peptide from protein
Sequence: EELMETIGMRLASQILSLVG
Position: 371-390
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P37
Chemical type: Peptide from protein
Sequence: QRGTSIRNIDPAIVSALWHS
Position: 391-410
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Positive-Low
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P38
Chemical type: Peptide from protein
Sequence: PAIVSALWHSLPEKLTTTNI
Position: 401-420
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Positive-Low
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P39
Chemical type: Peptide from protein
Sequence: LPEKLTTTNIKYDIASPTHM
Position: 411-430
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P40
Chemical type: Peptide from protein
Sequence: KYDIASPTHMSPALCTIFVQ
Position: 421-440
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P41
Chemical type: Peptide from protein
Sequence: SPALCTIFVQTGTSKQRFRN
Position: 431-450
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P42
Chemical type: Peptide from protein
Sequence: TGTSKQRFRNAGLLMVNNIF
Position: 441-460
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P43
Chemical type: Peptide from protein
Sequence: AGLLMVNNIFTVQARYSKQN
Position: 451-470
Source molecule (GenBank): APO37184.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P43
Chemical type: Peptide from protein
Sequence: AGLLMVNNIFTVQARYSKQN
Position: 451-470
Source molecule (GenBank): APO37184.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P43
Chemical type: Peptide from protein
Sequence: AGLLMVNNIFTVQARYSKQN
Position: 451-470
Source molecule (GenBank): APO37184.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P44
Chemical type: Peptide from protein
Sequence: TVQARYSKQNMFEKKIYGYE
Position: 461-480
Source molecule (GenBank): APO37184.1
Assay category: TCell
Result: Positive-Low
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P45
Chemical type: Peptide from protein
Sequence: MFEKKIYGYEHLGQALCEGG
Position: 54-73
Source molecule (GenBank): BAA78313.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P46
Chemical type: Peptide from protein
Sequence: HLGQALCEGGHVFYNPRDVY
Position: 64-83
Source molecule (GenBank): BAA78313.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P47
Chemical type: Peptide from protein
Sequence: HVFYNPRDVYFQNIKMAATE
Position: 74-93
Source molecule (GenBank): BAA78313.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: KPLTAMTAI
Chemical type: Peptide from protein
Sequence: KPLTAMTAI
Position: 193-201
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: KPLTAMTAI
Chemical type: Peptide from protein
Sequence: KPLTAMTAI
Position: 193-201
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: AFCPMTSKL
Chemical type: Peptide from protein
Sequence: AFCPMTSKL
Position: 202-210
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: AFCPMTSKL
Chemical type: Peptide from protein
Sequence: AFCPMTSKL
Position: 202-210
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IAFCPMTSKL
Chemical type: Peptide from protein
Sequence: IAFCPMTSKL
Position: 201-210
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: IAFCPMTSKL
Chemical type: Peptide from protein
Sequence: IAFCPMTSKL
Position: 201-210
Source molecule (GenBank): APO37529.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo