Reference: Janet Sim; Jonathan T Sockolosky; Emma Sangalang; Shelley Izquierdo; Darlene Pedersen; William Harriman; Ardian S Wibowo; Josh Carter; Anup Madan; Laura Doyle; Ons Harrabi; Steven E Kauder; Amy Chen; Tracy C Kuo; Hong Wan; Jaume Pons. "Discovery of high affinity, pan-allelic, and pan-mammalian reactive antibodies against the myeloid checkpoint receptor SIRPα." mAbs (2019).

Epitope: Epitope of 119 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: L60, I61, P62, V63, I66, W68, Y80, Q82, K83, E84, T92, S94, T97, K98, R99, N100, N101, D103, F104, S105, I106, R125, K126
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.342

Epitope: Epitope of 119 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: L60, I61, P62, V63, I66, W68, Y80, Q82, K83, E84, T92, S94, T97, K98, R99, N100, N101, D103, F104, S105, I106, R125, K126
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive

Epitope: Epitope of 119 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: L60, I61, P62, V63, I66, W68, Y80, Q82, K83, E84, T92, S94, T97, K98, R99, N100, N101, D103, F104, S105, I106, R125, K126
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive

Epitope: Epitope of 119 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: L60, I61, P62, V63, I66, W68, Y80, Q82, K83, E84, T92, S94, T97, K98, R99, N100, N101, D103, F104, S105, I106, R125, K126
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.0682

Epitope: Epitope of 119 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: L60, I61, P62, V63, I66, W68, Y80, Q82, K83, E84, T92, S94, T97, K98, R99, N100, N101, D103, F104, S105, I106, R125, K126
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.112

Epitope: Epitope of 119 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: L60, I61, P62, V63, I66, W68, Y80, Q82, K83, E84, T92, S94, T97, K98, R99, N100, N101, D103, F104, S105, I106, R125, K126
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.267

Epitope: Epitope of 119 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: L60, I61, P62, V63, I66, W68, Y80, Q82, K83, E84, T92, S94, T97, K98, R99, N100, N101, D103, F104, S105, I106, R125, K126
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.000395

Epitope: Epitope of 119 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: L60, I61, P62, V63, I66, W68, Y80, Q82, K83, E84, T92, S94, T97, K98, R99, N100, N101, D103, F104, S105, I106, R125, K126
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 2.42

Epitope: Epitope of 119 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: L60, I61, P62, V63, I66, W68, Y80, Q82, K83, E84, T92, S94, T97, K98, R99, N100, N101, D103, F104, S105, I106, R125, K126
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.183

Epitope: Epitope of 119 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: L60, I61, P62, V63, I66, W68, Y80, Q82, K83, E84, T92, S94, T97, K98, R99, N100, N101, D103, F104, S105, I106, R125, K126
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.000234

Epitope: Epitope of 119 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: L60, I61, P62, V63, I66, W68, Y80, Q82, K83, E84, T92, S94, T97, K98, R99, N100, N101, D103, F104, S105, I106, R125, K126
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.000428

Epitope: Epitope of 119 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: L60, I61, P62, V63, I66, W68, Y80, Q82, K83, E84, T92, S94, T97, K98, R99, N100, N101, D103, F104, S105, I106, R125, K126
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive

Epitope: Epitope of 119 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: L60, I61, P62, V63, I66, W68, Y80, Q82, K83, E84, T92, S94, T97, K98, R99, N100, N101, D103, F104, S105, I106, R125, K126
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.000148

Epitope: Epitope of 119 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: L60, I61, P62, V63, I66, W68, Y80, Q82, K83, E84, T92, S94, T97, K98, R99, N100, N101, D103, F104, S105, I106, R125, K126
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.000415

Epitope: Epitope of 136 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: Q38, P39, D40, K41, L44, A72, G73, T118, Y120, E133, S136, G137, A138, G139, E141, S143, A116
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.0000369

Epitope: Epitope of 136 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: Q38, P39, D40, K41, L44, A72, G73, T118, Y120, E133, S136, G137, A138, G139, E141, S143, A116
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.00179

Epitope: Epitope of 136 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: Q38, P39, D40, K41, L44, A72, G73, T118, Y120, E133, S136, G137, A138, G139, E141, S143, A116
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 2.15

Epitope: Epitope of 136 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: Q38, P39, D40, K41, L44, A72, G73, T118, Y120, E133, S136, G137, A138, G139, E141, S143, A116
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.00161

Epitope: Epitope of 136 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: Q38, P39, D40, K41, L44, A72, G73, T118, Y120, E133, S136, G137, A138, G139, E141, S143, A116
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.000386

Epitope: Epitope of 136 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: Q38, P39, D40, K41, L44, A72, G73, T118, Y120, E133, S136, G137, A138, G139, E141, S143, A116
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.0000334

Epitope: Epitope of 136 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: Q38, P39, D40, K41, L44, A72, G73, T118, Y120, E133, S136, G137, A138, G139, E141, S143, A116
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.458

Epitope: Epitope of 136 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: Q38, P39, D40, K41, L44, A72, G73, T118, Y120, E133, S136, G137, A138, G139, E141, S143, A116
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.000845

Epitope: Epitope of 136 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: Q38, P39, D40, K41, L44, A72, G73, T118, Y120, E133, S136, G137, A138, G139, E141, S143, A116
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.554

Epitope: Epitope of 136 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: Q38, P39, D40, K41, L44, A72, G73, T118, Y120, E133, S136, G137, A138, G139, E141, S143, A116
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 23.9

Epitope: Epitope of 136 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: Q38, P39, D40, K41, L44, A72, G73, T118, Y120, E133, S136, G137, A138, G139, E141, S143, A116
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 1.63

Epitope: Epitope of 136 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: Q38, P39, D40, K41, L44, A72, G73, T118, Y120, E133, S136, G137, A138, G139, E141, S143, A116
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 2.11

Epitope: Epitope of 136 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: Q38, P39, D40, K41, L44, A72, G73, T118, Y120, E133, S136, G137, A138, G139, E141, S143, A116
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive

Epitope: Epitope of 136 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: Q38, P39, D40, K41, L44, A72, G73, T118, Y120, E133, S136, G137, A138, G139, E141, S143, A116
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive

Epitope: Epitope of 136 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: Q38, P39, D40, K41, L44, A72, G73, T118, Y120, E133, S136, G137, A138, G139, E141, S143, A116
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 4.35

Epitope: Epitope of 136 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: Q38, P39, D40, K41, L44, A72, G73, T118, Y120, E133, S136, G137, A138, G139, E141, S143, A116
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Negative

Epitope: Epitope of 3 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: I37, Q38, P39, D40, K41, S42, T56, A138, G139, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 2.29

Epitope: Epitope of 3 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: I37, Q38, P39, D40, K41, S42, T56, A138, G139, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive-Low
Quantitative measurement: 0.01

Epitope: Epitope of 3 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: I37, Q38, P39, D40, K41, S42, T56, A138, G139, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.00192

Epitope: Epitope of 3 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: I37, Q38, P39, D40, K41, S42, T56, A138, G139, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.00218

Epitope: Epitope of 3 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: I37, Q38, P39, D40, K41, S42, T56, A138, G139, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 1.83

Epitope: Epitope of 3 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: I37, Q38, P39, D40, K41, S42, T56, A138, G139, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive

Epitope: Epitope of 3 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: I37, Q38, P39, D40, K41, S42, T56, A138, G139, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 23.9

Epitope: Epitope of 3 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: I37, Q38, P39, D40, K41, S42, T56, A138, G139, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.00041

Epitope: Epitope of 3 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: I37, Q38, P39, D40, K41, S42, T56, A138, G139, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.000276

Epitope: Epitope of 3 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: I37, Q38, P39, D40, K41, S42, T56, A138, G139, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.00119

Epitope: Epitope of 3 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: I37, Q38, P39, D40, K41, S42, T56, A138, G139, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 4.35

Epitope: Epitope of 3 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: I37, Q38, P39, D40, K41, S42, T56, A138, G139, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Negative

Epitope: Epitope of 3 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: I37, Q38, P39, D40, K41, S42, T56, A138, G139, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive

Epitope: Epitope of 3 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: I37, Q38, P39, D40, K41, S42, T56, A138, G139, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.458

Epitope: Epitope of 3 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: I37, Q38, P39, D40, K41, S42, T56, A138, G139, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 1.63

Epitope: Epitope of 3 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: I37, Q38, P39, D40, K41, S42, T56, A138, G139, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.554

Epitope: Epitope of 115 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: A47, R70, P74, G75, R76, E77, L78, I79, E84, G85, H86, F87, P88, R89, T112, P113, A114, D115
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.0000222

Epitope: Epitope of 115 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: A47, R70, P74, G75, R76, E77, L78, I79, E84, G85, H86, F87, P88, R89, T112, P113, A114, D115
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.0000334

Epitope: Epitope of 115 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: A47, R70, P74, G75, R76, E77, L78, I79, E84, G85, H86, F87, P88, R89, T112, P113, A114, D115
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.0000369

Epitope: Epitope of 115 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: A47, R70, P74, G75, R76, E77, L78, I79, E84, G85, H86, F87, P88, R89, T112, P113, A114, D115
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.00000786

Epitope: Epitope of 115 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: A47, R70, P74, G75, R76, E77, L78, I79, E84, G85, H86, F87, P88, R89, T112, P113, A114, D115
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.0000195

Epitope: Epitope of 115 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: A47, R70, P74, G75, R76, E77, L78, I79, E84, G85, H86, F87, P88, R89, T112, P113, A114, D115
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive

Epitope: Epitope of 115 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: A47, R70, P74, G75, R76, E77, L78, I79, E84, G85, H86, F87, P88, R89, T112, P113, A114, D115
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 2.55

Epitope: Epitope of 115 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: A47, R70, P74, G75, R76, E77, L78, I79, E84, G85, H86, F87, P88, R89, T112, P113, A114, D115
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 1.86

Epitope: Epitope of 115 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: A47, R70, P74, G75, R76, E77, L78, I79, E84, G85, H86, F87, P88, R89, T112, P113, A114, D115
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 2.41

Epitope: Epitope of 115 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: A47, R70, P74, G75, R76, E77, L78, I79, E84, G85, H86, F87, P88, R89, T112, P113, A114, D115
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive

Epitope: Epitope of 115 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: A47, R70, P74, G75, R76, E77, L78, I79, E84, G85, H86, F87, P88, R89, T112, P113, A114, D115
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.426

Epitope: Epitope of 218 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: F69, R70, A72, G73, P74, G75, R76, E77, G117, T118, V132, E133, S136, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Negative

Epitope: Epitope of 218 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: F69, R70, A72, G73, P74, G75, R76, E77, G117, T118, V132, E133, S136, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.276

Epitope: Epitope of 218 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: F69, R70, A72, G73, P74, G75, R76, E77, G117, T118, V132, E133, S136, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.0599

Epitope: Epitope of 218 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: F69, R70, A72, G73, P74, G75, R76, E77, G117, T118, V132, E133, S136, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.0636

Epitope: Epitope of 218 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: F69, R70, A72, G73, P74, G75, R76, E77, G117, T118, V132, E133, S136, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive

Epitope: Epitope of 218 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: F69, R70, A72, G73, P74, G75, R76, E77, G117, T118, V132, E133, S136, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.0000547

Epitope: Epitope of 218 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: F69, R70, A72, G73, P74, G75, R76, E77, G117, T118, V132, E133, S136, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive

Epitope: Epitope of 218 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: F69, R70, A72, G73, P74, G75, R76, E77, G117, T118, V132, E133, S136, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 2.42

Epitope: Epitope of 218 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: F69, R70, A72, G73, P74, G75, R76, E77, G117, T118, V132, E133, S136, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.123

Epitope: Epitope of 218 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: F69, R70, A72, G73, P74, G75, R76, E77, G117, T118, V132, E133, S136, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.0000706

Epitope: Epitope of 218 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: F69, R70, A72, G73, P74, G75, R76, E77, G117, T118, V132, E133, S136, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.0000249

Epitope: Epitope of 218 Fab on SIRPα
Chemical type: Discontinuous protein residues
Discontinuous residues: F69, R70, A72, G73, P74, G75, R76, E77, G117, T118, V132, E133, S136, E141
Source molecule (GenBank): NP_542970.1
Assay category: BCell
Result: Positive
Quantitative measurement: 0.0000212