Reference: C Bartholdy; S L Reedtz-Runge; J Wang; L Hjerrild Zeuthen; A Gruhler; C N Gudme; K Lamberth. "In silico and in vitro immunogenicity assessment of B-domain-modified recombinant factor VIII molecules." Haemophilia : the official journal of the World Federation of Hemophilia (2018).

Epitope: Turoctocog alfa (NovoEight)
Chemical type: Peptide, no natural source
Sequence: PRSFSQNSRHPSQNPPVLKRHQRE
Assay category: TCell
Result: Negative
Cell type: T cell CD8-
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Turoctocog alfa (NovoEight)
Chemical type: Peptide, no natural source
Sequence: PRSFSQNSRHPSQNPPVLKRHQRE
Assay category: TCell
Result: Negative
Cell type: T cell CD8-
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Turoctocog alfa (NovoEight)
Chemical type: Peptide, no natural source
Sequence: PRSFSQNSRHPSQNPPVLKRHQRE
Assay category: MhcBinding
Result: Negative

Epitope: Turoctocog alfa (NovoEight)
Chemical type: Peptide, no natural source
Sequence: PRSFSQNSRHPSQNPPVLKRHQRE
Assay category: MhcBinding
Result: Positive-Low

Epitope: Turoctocog alfa (NovoEight)
Chemical type: Peptide, no natural source
Sequence: PRSFSQNSRHPSQNPPVLKRHQRE
Assay category: MhcBinding
Result: Negative

Epitope: Turoctocog alfa (NovoEight)
Chemical type: Peptide, no natural source
Sequence: PRSFSQNSRHPSQNPPVLKRHQRE
Assay category: MhcBinding
Result: Negative

Epitope: Turoctocog alfa (NovoEight)
Chemical type: Peptide, no natural source
Sequence: PRSFSQNSRHPSQNPPVLKRHQRE
Assay category: MhcBinding
Result: Negative

Epitope: Turoctocog alfa (NovoEight)
Chemical type: Peptide, no natural source
Sequence: PRSFSQNSRHPSQNPPVLKRHQRE
Assay category: MhcBinding
Result: Negative

Epitope: Turoctocog alfa (NovoEight)
Chemical type: Peptide, no natural source
Sequence: PRSFSQNSRHPSQNPPVLKRHQRE
Assay category: MhcBinding
Result: Positive

Epitope: Turoctocog alfa (NovoEight)
Chemical type: Peptide, no natural source
Sequence: PRSFSQNSRHPSQNPPVLKRHQRE
Assay category: MhcBinding
Result: Negative

Epitope: Turoctocog alfa (NovoEight)
Chemical type: Peptide, no natural source
Sequence: PRSFSQNSRHPSQNPPVLKRHQRE
Assay category: MhcBinding
Result: Negative

Epitope: Turoctocog alfa (NovoEight)
Chemical type: Peptide, no natural source
Sequence: PRSFSQNSRHPSQNPPVLKRHQRE
Assay category: MhcBinding
Result: Negative

Epitope: Simoctocog alfa (Nuwiq)
Chemical type: Peptide, no natural source
Sequence: IEPRSFSQNSRHQAYRYRRGEITRTTLQSDQE
Assay category: TCell
Result: Negative
Cell type: T cell CD8-
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Simoctocog alfa (Nuwiq)
Chemical type: Peptide, no natural source
Sequence: IEPRSFSQNSRHQAYRYRRGEITRTTLQSDQE
Assay category: TCell
Result: Negative
Cell type: T cell CD8-
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Simoctocog alfa (Nuwiq)
Chemical type: Peptide, no natural source
Sequence: IEPRSFSQNSRHQAYRYRRGEITRTTLQSDQE
Assay category: MhcBinding
Result: Positive

Epitope: Simoctocog alfa (Nuwiq)
Chemical type: Peptide, no natural source
Sequence: IEPRSFSQNSRHQAYRYRRGEITRTTLQSDQE
Assay category: MhcBinding
Result: Positive

Epitope: Simoctocog alfa (Nuwiq)
Chemical type: Peptide, no natural source
Sequence: IEPRSFSQNSRHQAYRYRRGEITRTTLQSDQE
Assay category: MhcBinding
Result: Positive-Low

Epitope: Simoctocog alfa (Nuwiq)
Chemical type: Peptide, no natural source
Sequence: IEPRSFSQNSRHQAYRYRRGEITRTTLQSDQE
Assay category: MhcBinding
Result: Negative

Epitope: Simoctocog alfa (Nuwiq)
Chemical type: Peptide, no natural source
Sequence: IEPRSFSQNSRHQAYRYRRGEITRTTLQSDQE
Assay category: MhcBinding
Result: Negative

Epitope: Simoctocog alfa (Nuwiq)
Chemical type: Peptide, no natural source
Sequence: IEPRSFSQNSRHQAYRYRRGEITRTTLQSDQE
Assay category: MhcBinding
Result: Positive-Low

Epitope: Simoctocog alfa (Nuwiq)
Chemical type: Peptide, no natural source
Sequence: IEPRSFSQNSRHQAYRYRRGEITRTTLQSDQE
Assay category: MhcBinding
Result: Positive

Epitope: Simoctocog alfa (Nuwiq)
Chemical type: Peptide, no natural source
Sequence: IEPRSFSQNSRHQAYRYRRGEITRTTLQSDQE
Assay category: MhcBinding
Result: Positive-Low

Epitope: Simoctocog alfa (Nuwiq)
Chemical type: Peptide, no natural source
Sequence: IEPRSFSQNSRHQAYRYRRGEITRTTLQSDQE
Assay category: MhcBinding
Result: Negative

Epitope: Simoctocog alfa (Nuwiq)
Chemical type: Peptide, no natural source
Sequence: IEPRSFSQNSRHQAYRYRRGEITRTTLQSDQE
Assay category: MhcBinding
Result: Negative

Epitope: Moroctocog alfa (ReFacto)
Chemical type: Peptide, no natural source
Sequence: SKNNAIEPRSFSQNPPVLKRHQRE
Assay category: TCell
Result: Negative
Cell type: T cell CD8-
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Moroctocog alfa (ReFacto)
Chemical type: Peptide, no natural source
Sequence: SKNNAIEPRSFSQNPPVLKRHQRE
Assay category: TCell
Result: Negative
Cell type: T cell CD8-
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Moroctocog alfa (ReFacto)
Chemical type: Peptide, no natural source
Sequence: SKNNAIEPRSFSQNPPVLKRHQRE
Assay category: MhcBinding
Result: Negative

Epitope: Moroctocog alfa (ReFacto)
Chemical type: Peptide, no natural source
Sequence: SKNNAIEPRSFSQNPPVLKRHQRE
Assay category: MhcBinding
Result: Negative

Epitope: Moroctocog alfa (ReFacto)
Chemical type: Peptide, no natural source
Sequence: SKNNAIEPRSFSQNPPVLKRHQRE
Assay category: MhcBinding
Result: Negative

Epitope: Moroctocog alfa (ReFacto)
Chemical type: Peptide, no natural source
Sequence: SKNNAIEPRSFSQNPPVLKRHQRE
Assay category: MhcBinding
Result: Positive

Epitope: Moroctocog alfa (ReFacto)
Chemical type: Peptide, no natural source
Sequence: SKNNAIEPRSFSQNPPVLKRHQRE
Assay category: MhcBinding
Result: Negative

Epitope: Moroctocog alfa (ReFacto)
Chemical type: Peptide, no natural source
Sequence: SKNNAIEPRSFSQNPPVLKRHQRE
Assay category: MhcBinding
Result: Negative

Epitope: Moroctocog alfa (ReFacto)
Chemical type: Peptide, no natural source
Sequence: SKNNAIEPRSFSQNPPVLKRHQRE
Assay category: MhcBinding
Result: Negative

Epitope: Moroctocog alfa (ReFacto)
Chemical type: Peptide, no natural source
Sequence: SKNNAIEPRSFSQNPPVLKRHQRE
Assay category: MhcBinding
Result: Negative

Epitope: Moroctocog alfa (ReFacto)
Chemical type: Peptide, no natural source
Sequence: SKNNAIEPRSFSQNPPVLKRHQRE
Assay category: MhcBinding
Result: Positive-Low

Epitope: Moroctocog alfa (ReFacto)
Chemical type: Peptide, no natural source
Sequence: SKNNAIEPRSFSQNPPVLKRHQRE
Assay category: MhcBinding
Result: Negative

Epitope: F VIII (102-114)
Chemical type: Peptide from protein
Sequence: TVVITLKNMASHP
Position: 102-114
Source molecule (GenBank): P00451.1
Assay category: MhcLigandElution
Result: Positive

Epitope: F VIII (127-140)
Chemical type: Peptide from protein
Sequence: ASEGAEYDDQTSQR
Position: 127-140
Source molecule (GenBank): P00451.1
Assay category: MhcLigandElution
Result: Positive

Epitope: F VIII (232-245)
Chemical type: Peptide from protein
Sequence: KNSLMQDRDAASAR
Position: 232-245
Source molecule (GenBank): P00451.1
Assay category: MhcLigandElution
Result: Positive

Epitope: F VIII (308-319)
Chemical type: Peptide from protein
Sequence: SPITFLTAQTLL
Position: 308-319
Source molecule (GenBank): P00451.1
Assay category: MhcLigandElution
Result: Positive

Epitope: F VIII (481-491)
Chemical type: Peptide from protein
Sequence: LIIFKNQASRP
Position: 481-491
Source molecule (GenBank): P00451.1
Assay category: MhcLigandElution
Result: Positive

Epitope: F VIII (552-565)
Chemical type: Peptide from protein
Sequence: YSSFVNMERDLASG
Position: 552-565
Source molecule (GenBank): P00451.1
Assay category: MhcLigandElution
Result: Positive

Epitope: F VIII (582-594)
Chemical type: Peptide from protein
Sequence: GNQIMSDKRNVIL
Position: 582-594
Source molecule (GenBank): P00451.1
Assay category: MhcLigandElution
Result: Positive

Epitope: F VIII (610-622)
Chemical type: Peptide from protein
Sequence: IQRFLPNPAGVQL
Position: 610-622
Source molecule (GenBank): P00451.1
Assay category: MhcLigandElution
Result: Positive

Epitope: F VIII (1715-1727)
Chemical type: Peptide from protein
Sequence: RHYFIAAVERLWD
Position: 1715-1727
Source molecule (GenBank): P00451.1
Assay category: MhcLigandElution
Result: Positive

Epitope: F VIII (1728-1739)
Chemical type: Peptide from protein
Sequence: YGMSSSPHVLRN
Position: 1728-1739
Source molecule (GenBank): P00451.1
Assay category: MhcLigandElution
Result: Positive

Epitope: F VIII (1788-1802)
Chemical type: Peptide from protein
Sequence: DNIMVTFRNQASRPY
Position: 1788-1802
Source molecule (GenBank): P00451.1
Assay category: MhcLigandElution
Result: Positive

Epitope: F VIII (1940-1952)
Chemical type: Peptide from protein
Sequence: INGYIMDTLPGLV
Position: 1940-1952
Source molecule (GenBank): P00451.1
Assay category: MhcLigandElution
Result: Positive

Epitope: F VIII (1998-2011)
Chemical type: Peptide from protein
Sequence: YPGVFETVEMLPSK
Position: 1998-2011
Source molecule (GenBank): P00451.1
Assay category: MhcLigandElution
Result: Positive

Epitope: F VIII (2014-2027)
Chemical type: Peptide from protein
Sequence: IWRVECLIGEHLHA
Position: 2014-2027
Source molecule (GenBank): P00451.1
Assay category: MhcLigandElution
Result: Positive

Epitope: F VIII (2085-2098)
Chemical type: Peptide from protein
Sequence: EPFSWIKVDLLAPM
Position: 2085-2098
Source molecule (GenBank): P00451.1
Assay category: MhcLigandElution
Result: Positive

Epitope: F VIII (2096-2109)
Chemical type: Peptide from protein
Sequence: APMIIHGIKTQGAR
Position: 2096-2109
Source molecule (GenBank): P00451.1
Assay category: MhcLigandElution
Result: Positive

Epitope: F VIII (2117-2129)
Chemical type: Peptide from protein
Sequence: ISQFIIMYSLDGK
Position: 2117-2129
Source molecule (GenBank): P00451.1
Assay category: MhcLigandElution
Result: Positive

Epitope: F VIII (2124-2130)
Chemical type: Peptide from protein
Sequence: SLDGKK
Position: 2125-2130
Source molecule (GenBank): P00451.1
Assay category: MhcLigandElution
Result: Positive

Epitope: F VIII (2244-2261)
Chemical type: Peptide from protein
Sequence: NPKEWLQVDFQKTMKVTG
Position: 2244-2261
Source molecule (GenBank): P00451.1
Assay category: MhcLigandElution
Result: Positive

Epitope: F VIII (2277-2289)
Chemical type: Peptide from protein
Sequence: KEFLISSSQDGHQ
Position: 2277-2289
Source molecule (GenBank): P00451.1
Assay category: MhcLigandElution
Result: Positive

Epitope: F VIII (2328-2340)
Chemical type: Peptide from protein
Sequence: HPQSWVHQIALRM
Position: 2328-2340
Source molecule (GenBank): P00451.1
Assay category: MhcLigandElution
Result: Positive