Reference: Hanaa Saeed; Christine Gagnon; Elroy Cober; Steve Gleddie. "Using patient serum to epitope map soybean glycinins reveals common epitopes shared with many legumes and tree nuts." Molecular immunology (2016).

Epitope: Gly m 6 A3 25-36
Chemical type: Peptide from protein
Sequence: ITSSKFNECQLN
Position: 25-36
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 28-39
Chemical type: Peptide from protein
Sequence: SKFNECQLNNLN
Position: 28-39
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 31-42
Chemical type: Peptide from protein
Sequence: NECQLNNLNALE
Position: 31-42
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 34-45
Chemical type: Peptide from protein
Sequence: QLNNLNALEPDH
Position: 34-45
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 37-48
Chemical type: Peptide from protein
Sequence: NLNALEPDHRVE
Position: 37-48
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 40-51
Chemical type: Peptide from protein
Sequence: ALEPDHRVESEG
Position: 40-51
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 43-54
Chemical type: Peptide from protein
Sequence: PDHRVESEGGLI
Position: 43-54
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 49-60
Chemical type: Peptide from protein
Sequence: SEGGLIETWNSQ
Position: 49-60
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 52-63
Chemical type: Peptide from protein
Sequence: GLIETWNSQHPE
Position: 52-63
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 55-66
Chemical type: Peptide from protein
Sequence: ETWNSQHPELQC
Position: 55-66
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 58-69
Chemical type: Peptide from protein
Sequence: NSQHPELQCAGV
Position: 58-69
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 61-72
Chemical type: Peptide from protein
Sequence: HPELQCAGVTVS
Position: 61-72
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 64-75
Chemical type: Peptide from protein
Sequence: LQCAGVTVSKRT
Position: 64-75
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 67-78
Chemical type: Peptide from protein
Sequence: AGVTVSKRTLNR
Position: 67-78
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 70-81
Chemical type: Peptide from protein
Sequence: TVSKRTLNRNGL
Position: 70-81
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 73-84
Chemical type: Peptide from protein
Sequence: KRTLNRNGLHLP
Position: 73-84
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 76-87
Chemical type: Peptide from protein
Sequence: LNRNGLHLPSYS
Position: 76-87
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 79-90
Chemical type: Peptide from protein
Sequence: NGLHLPSYSPYP
Position: 79-90
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 85-96
Chemical type: Peptide from protein
Sequence: SYSPYPQMIIVV
Position: 85-96
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 88-99
Chemical type: Peptide from protein
Sequence: PYPQMIIVVQGK
Position: 88-99
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 91-102
Chemical type: Peptide from protein
Sequence: QMIIVVQGKGAI
Position: 91-102
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 94-105
Chemical type: Peptide from protein
Sequence: IVVQGKGAIGFA
Position: 94-105
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 100-111
Chemical type: Peptide from protein
Sequence: GAIGFAFPGCPE
Position: 100-111
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 103-114
Chemical type: Peptide from protein
Sequence: GFAFPGCPETFE
Position: 103-114
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 106-117
Chemical type: Peptide from protein
Sequence: FPGCPETFEKPQ
Position: 106-117
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 109-120
Chemical type: Peptide from protein
Sequence: CPETFEKPQQQS
Position: 109-120
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 112-123
Chemical type: Peptide from protein
Sequence: TFEKPQQQSSRR
Position: 112-123
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 115-126
Chemical type: Peptide from protein
Sequence: KPQQQSSRRGSR
Position: 115-126
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 118-129
Chemical type: Peptide from protein
Sequence: QQSSRRGSRSQQ
Position: 118-129
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 121-132
Chemical type: Peptide from protein
Sequence: SRRGSRSQQQLQ
Position: 121-132
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 124-135
Chemical type: Peptide from protein
Sequence: GSRSQQQLQDSH
Position: 124-135
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 127-138
Chemical type: Peptide from protein
Sequence: SQQQLQDSHQKI
Position: 127-138
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 130-141
Chemical type: Peptide from protein
Sequence: QLQDSHQKIRHF
Position: 130-141
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 133-144
Chemical type: Peptide from protein
Sequence: DSHQKIRHFNEG
Position: 133-144
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 136-147
Chemical type: Peptide from protein
Sequence: QKIRHFNEGDVL
Position: 136-147
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 139-150
Chemical type: Peptide from protein
Sequence: RHFNEGDVLVIP
Position: 139-150
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 142-153
Chemical type: Peptide from protein
Sequence: NEGDVLVIPPGV
Position: 142-153
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 145-156
Chemical type: Peptide from protein
Sequence: DVLVIPPGVPYW
Position: 145-156
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 148-159
Chemical type: Peptide from protein
Sequence: VIPPGVPYWTYN
Position: 148-159
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 151-162
Chemical type: Peptide from protein
Sequence: PGVPYWTYNTGD
Position: 151-162
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 154-165
Chemical type: Peptide from protein
Sequence: PYWTYNTGDEPV
Position: 154-165
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 157-168
Chemical type: Peptide from protein
Sequence: TYNTGDEPVVAI
Position: 157-168
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 160-171
Chemical type: Peptide from protein
Sequence: TGDEPVVAISLL
Position: 160-171
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 163-174
Chemical type: Peptide from protein
Sequence: EPVVAISLLDTS
Position: 163-174
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 166-177
Chemical type: Peptide from protein
Sequence: VAISLLDTSNFN
Position: 166-177
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 169-180
Chemical type: Peptide from protein
Sequence: SLLDTSNFNNQL
Position: 169-180
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 172-183
Chemical type: Peptide from protein
Sequence: DTSNFNNQLDQN
Position: 172-183
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 175-186
Chemical type: Peptide from protein
Sequence: NFNNQLDQNPRV
Position: 175-186
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 178-189
Chemical type: Peptide from protein
Sequence: NQLDQNPRVFYL
Position: 178-189
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 181-192
Chemical type: Peptide from protein
Sequence: DQNPRVFYLAGN
Position: 181-192
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 184-195
Chemical type: Peptide from protein
Sequence: PRVFYLAGNPDI
Position: 184-195
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 187-198
Chemical type: Peptide from protein
Sequence: FYLAGNPDIEHP
Position: 187-198
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 190-201
Chemical type: Peptide from protein
Sequence: AGNPDIEHPETM
Position: 190-201
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 193-2014
Chemical type: Peptide from protein
Sequence: PDIEHPETMQQQ
Position: 193-204
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 196-207
Chemical type: Peptide from protein
Sequence: EHPETMQQQQQQ
Position: 196-207
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 199-210
Chemical type: Peptide from protein
Sequence: ETMQQQQQQKSH
Position: 199-210
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 202-213
Chemical type: Peptide from protein
Sequence: QQQQQQKSHGGR
Position: 202-213
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 205-216
Chemical type: Peptide from protein
Sequence: QQQKSHGGRKQG
Position: 205-216
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 217-228
Chemical type: Peptide from protein
Sequence: QHQQQEEEGGSV
Position: 217-228
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 220-231
Chemical type: Peptide from protein
Sequence: QQEEEGGSVLSG
Position: 220-231
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 226-237
Chemical type: Peptide from protein
Sequence: GSVLSGFSKHFL
Position: 226-237
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Positive
Host: F 23-38 years

Epitope: Gly m 6 A3 229-240
Chemical type: Peptide from protein
Sequence: LSGFSKHFLAQS
Position: 229-240
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 232-243
Chemical type: Peptide from protein
Sequence: FSKHFLAQSFNT
Position: 232-243
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 235-246
Chemical type: Peptide from protein
Sequence: HFLAQSFNTNED
Position: 235-246
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 238-249
Chemical type: Peptide from protein
Sequence: AQSFNTNEDTAE
Position: 238-249
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 241-252
Chemical type: Peptide from protein
Sequence: FNTNEDTAEKLR
Position: 241-252
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 244-255
Chemical type: Peptide from protein
Sequence: NEDTAEKLRSPD
Position: 244-255
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 250-261
Chemical type: Peptide from protein
Sequence: KLRSPDDERKQI
Position: 250-261
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 253-264
Chemical type: Peptide from protein
Sequence: SPDDERKQIVTV
Position: 253-264
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 256-267
Chemical type: Peptide from protein
Sequence: DERKQIVTVEGG
Position: 256-267
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 259-270
Chemical type: Peptide from protein
Sequence: KQIVTVEGGLSV
Position: 259-270
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 262-273
Chemical type: Peptide from protein
Sequence: VTVEGGLSVISP
Position: 262-273
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 265-276
Chemical type: Peptide from protein
Sequence: EGGLSVISPKWQ
Position: 265-276
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 271-282
Chemical type: Peptide from protein
Sequence: ISPKWQEQEDED
Position: 271-282
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 274-285
Chemical type: Peptide from protein
Sequence: KWQEQEDEDEDE
Position: 274-285
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 277-288
Chemical type: Peptide from protein
Sequence: EQEDEDEDEDEE
Position: 277-288
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 280-291
Chemical type: Peptide from protein
Sequence: DEDEDEDEEYEQ
Position: 280-291
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 283-294
Chemical type: Peptide from protein
Sequence: EDEDEEYEQTPS
Position: 283-294
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 286-297
Chemical type: Peptide from protein
Sequence: DEEYEQTPSYPP
Position: 286-297
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 289-300
Chemical type: Peptide from protein
Sequence: YEQTPSYPPRRP
Position: 289-300
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 292-303
Chemical type: Peptide from protein
Sequence: TPSYPPRRPSHG
Position: 292-303
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 295-306
Chemical type: Peptide from protein
Sequence: YPPRRPSHGKHE
Position: 295-306
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 298-309
Chemical type: Peptide from protein
Sequence: RRPSHGKHEDDE
Position: 298-309
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 301-312
Chemical type: Peptide from protein
Sequence: SHGKHEDDEDED
Position: 301-312
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 304-315
Chemical type: Peptide from protein
Sequence: KHEDDEDEDEEE
Position: 304-315
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 307-318
Chemical type: Peptide from protein
Sequence: DDEDEDEEEDQP
Position: 307-318
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 310-321
Chemical type: Peptide from protein
Sequence: DEDEEEDQPRPD
Position: 310-321
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 313-324
Chemical type: Peptide from protein
Sequence: EEEDQPRPDHPP
Position: 313-324
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Positive
Host: F 23-38 years

Epitope: Gly m 6 A3 319-330
Chemical type: Peptide from protein
Sequence: RPDHPPQRPSRP
Position: 319-330
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 325-336
Chemical type: Peptide from protein
Sequence: QRPSRPEQQEPR
Position: 325-336
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 328-339
Chemical type: Peptide from protein
Sequence: SRPEQQEPRGRG
Position: 328-339
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 331-342
Chemical type: Peptide from protein
Sequence: EQQEPRGRGCQT
Position: 331-342
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A3 334-344
Chemical type: Peptide from protein
Sequence: EPRGRGCQTRN
Position: 334-344
Source molecule (GenBank): BAB15802.1
Assay category: BCell
Result: Negative
Host: F 23-38 years

Epitope: Gly m 6 A2 121-129
Chemical type: Peptide from protein
Sequence: QRPQDRHQK
Position: 121-129
Source molecule (GenBank): P04405.2
Assay category: BCell
Result: Positive
Host:  18-33 years

Epitope: Gly m 6 A2 130-141
Chemical type: Peptide from protein
Sequence: VHRFREGDLIAV
Position: 130-141
Source molecule (GenBank): P04405.2
Assay category: BCell
Result: Positive
Host:  18-33 years

Epitope: Gly m 6 A2 136-153
Chemical type: Peptide from protein
Sequence: GDLIAVPTGVAWWMYNNE
Position: 136-153
Source molecule (GenBank): P04405.2
Assay category: BCell
Result: Positive
Host:  18-33 years

Epitope: Gly m 6 A2 214-225
Chemical type: Peptide from protein
Sequence: GSNILSGFAPEF
Position: 214-225
Source molecule (GenBank): P04405.2
Assay category: BCell
Result: Positive
Host:  18-33 years