Reference: Junqi Zhang; Baozeng Sun; Wenyang Shen; Zhenjie Wang; Yang Liu; Yubo Sun; Jiaxing Zhang; Ruibo Liu; Yongkai Wang; Tianyuan Bai; Zilu Ma; Cheng Luo; Xupeng Qiao; Xiyang Zhang; Shuya Yang; Yuanjie Sun; Dongbo Jiang; Kun Yang. "Analyses, Experimental Verification and Application in DNA Vaccines of Ebolavirus GP-Derived pan-MHC-II-Restricted Epitopes." Vaccines (2023).

Epitope: 1
Chemical type: Peptide from protein
Sequence: EAAVSHLTTLATIST
Position: 359-373
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 1
Chemical type: Peptide from protein
Sequence: EAAVSHLTTLATIST
Position: 359-373
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive-Low
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 1
Chemical type: Peptide from protein
Sequence: EAAVSHLTTLATIST
Position: 359-373
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 2
Chemical type: Peptide from protein
Sequence: GRRARREAIVNAQPK
Position: 496-510
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 2
Chemical type: Peptide from protein
Sequence: GRRARREAIVNAQPK
Position: 496-510
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 2
Chemical type: Peptide from protein
Sequence: GRRARREAIVNAQPK
Position: 496-510
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 4
Chemical type: Peptide from protein
Sequence: LFLRATTELRTFSIL
Position: 571-585
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive-High
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 4
Chemical type: Peptide from protein
Sequence: LFLRATTELRTFSIL
Position: 571-585
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 4
Chemical type: Peptide from protein
Sequence: LFLRATTELRTFSIL
Position: 571-585
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive-Low
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 5
Chemical type: Peptide from protein
Sequence: FLYDRLASTVIYRGT
Position: 160-174
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 5
Chemical type: Peptide from protein
Sequence: FLYDRLASTVIYRGT
Position: 160-174
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive-Low
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 5
Chemical type: Peptide from protein
Sequence: FLYDRLASTVIYRGT
Position: 160-174
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 6
Chemical type: Peptide from protein
Sequence: QVHSQGREAAVSHLT
Position: 352-366
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 6
Chemical type: Peptide from protein
Sequence: QVHSQGREAAVSHLT
Position: 352-366
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 6
Chemical type: Peptide from protein
Sequence: QVHSQGREAAVSHLT
Position: 352-366
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 7
Chemical type: Peptide from protein
Sequence: IRSEELSFTAVSNRA
Position: 301-315
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 7
Chemical type: Peptide from protein
Sequence: IRSEELSFTAVSNRA
Position: 301-315
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive-Low
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 7
Chemical type: Peptide from protein
Sequence: IRSEELSFTAVSNRA
Position: 301-315
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 9
Chemical type: Peptide from protein
Sequence: GLITNTIAGVAGLIT
Position: 480-494
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 9
Chemical type: Peptide from protein
Sequence: GLITNTIAGVAGLIT
Position: 480-494
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive-Low
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 9
Chemical type: Peptide from protein
Sequence: GLITNTIAGVAGLIT
Position: 480-494
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 10
Chemical type: Peptide from protein
Sequence: IGEWAFWETKKNLTR
Position: 285-299
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 10
Chemical type: Peptide from protein
Sequence: IGEWAFWETKKNLTR
Position: 285-299
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 10
Chemical type: Peptide from protein
Sequence: IGEWAFWETKKNLTR
Position: 285-299
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive-Low
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 11
Chemical type: Peptide from protein
Sequence: EGAFFLYDRLASTVI
Position: 156-170
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 11
Chemical type: Peptide from protein
Sequence: EGAFFLYDRLASTVI
Position: 156-170
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive-Low
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 11
Chemical type: Peptide from protein
Sequence: EGAFFLYDRLASTVI
Position: 156-170
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 12
Chemical type: Peptide from protein
Sequence: ELRTFSILNRKAIDF
Position: 578-592
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 12
Chemical type: Peptide from protein
Sequence: ELRTFSILNRKAIDF
Position: 578-592
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 12
Chemical type: Peptide from protein
Sequence: ELRTFSILNRKAIDF
Position: 578-592
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive-Low
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 13
Chemical type: Peptide from protein
Sequence: SATKRWGFRSGVPPK
Position: 81-95
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 13
Chemical type: Peptide from protein
Sequence: SATKRWGFRSGVPPK
Position: 81-95
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive-Low
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 13
Chemical type: Peptide from protein
Sequence: SATKRWGFRSGVPPK
Position: 81-95
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 14
Chemical type: Peptide from protein
Sequence: PSSGYYSTTIRYQAT
Position: 209-223
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 14
Chemical type: Peptide from protein
Sequence: PSSGYYSTTIRYQAT
Position: 209-223
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 14
Chemical type: Peptide from protein
Sequence: PSSGYYSTTIRYQAT
Position: 209-223
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive-Low
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 15
Chemical type: Peptide from protein
Sequence: TQALQLFLRATTELR
Position: 566-580
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 15
Chemical type: Peptide from protein
Sequence: TQALQLFLRATTELR
Position: 566-580
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 15
Chemical type: Peptide from protein
Sequence: TQALQLFLRATTELR
Position: 566-580
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 16
Chemical type: Peptide from protein
Sequence: VVAFLILPQAKKDFF
Position: 180-194
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 16
Chemical type: Peptide from protein
Sequence: VVAFLILPQAKKDFF
Position: 180-194
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 16
Chemical type: Peptide from protein
Sequence: VVAFLILPQAKKDFF
Position: 180-194
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive-Low
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 17
Chemical type: Peptide from protein
Sequence: IDFLLQRWGGTCHIL
Position: 590-604
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 17
Chemical type: Peptide from protein
Sequence: IDFLLQRWGGTCHIL
Position: 590-604
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive-Low
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 17
Chemical type: Peptide from protein
Sequence: IDFLLQRWGGTCHIL
Position: 590-604
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 18
Chemical type: Peptide from protein
Sequence: GIRGFPRCRYVHKVS
Position: 128-142
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 18
Chemical type: Peptide from protein
Sequence: GIRGFPRCRYVHKVS
Position: 128-142
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: 18
Chemical type: Peptide from protein
Sequence: GIRGFPRCRYVHKVS
Position: 128-142
Source molecule (GenBank): AAQ55048.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo