Reference: Jewell N Walters; Bernadette Ferraro; Elizabeth K Duperret; Kimberly A Kraynyak; Jaemi Chu; Ashley Saint-Fleur; Jian Yan; Hy Levitsky; Amir S Khan; Niranjan Y Sardesai; David B Weiner. "A Novel DNA Vaccine Platform Enhances Neo-antigen-like T Cell Responses against WT1 to Break Tolerance and Induce Anti-tumor Immunity." Molecular therapy : the journal of the American Society of Gene Therapy (2017).

Epitope: WT1-S cons P1
Chemical type: Peptide, no natural source
Sequence: AAPYGSLGGPHSFIK
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P2
Chemical type: Peptide, no natural source
Sequence: LGGPHSFIKQEPSWG
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P3
Chemical type: Peptide, no natural source
Sequence: FIKQEPSWGGADPHE
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P5
Chemical type: Peptide, no natural source
Sequence: PHEEQCLSAFTVHFS
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P6
Chemical type: Peptide, no natural source
Sequence: CRYGPFGAPPPSQAP
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P7
Chemical type: Peptide, no natural source
Sequence: GAPPPSQAPSGQARM
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P8
Chemical type: Peptide, no natural source
Sequence: QAPSGQARMFPNAPY
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P9
Chemical type: Peptide, no natural source
Sequence: ARMFPNAPYLPNCLE
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P10
Chemical type: Peptide, no natural source
Sequence: APYLPNCLESQPAIR
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P11
Chemical type: Peptide, no natural source
Sequence: DGTPSYGHTPSHHAA
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P16
Chemical type: Peptide, no natural source
Sequence: ATGYESDNHTTPMLY
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P18
Chemical type: Peptide, no natural source
Sequence: ML YSCGAQYRIHTHG
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S P21
Chemical type: Peptide from protein
Sequence: AGACRYGPFGPPPPS
Position: 104-118
Source molecule (GenBank): P22561.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S P22
Chemical type: Peptide from protein
Sequence: PPSQASSGQARMFPN
Position: 116-130
Source molecule (GenBank): P22561.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S P23
Chemical type: Peptide from protein
Sequence: SGQARMFPNAPYLPS
Position: 122-136
Source molecule (GenBank): P22561.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S P24
Chemical type: Peptide from protein
Sequence: FPNAPYLPSCLESQP
Position: 128-142
Source molecule (GenBank): P22561.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S P25
Chemical type: Peptide from protein
Sequence: VTFDGAPSYGHTPSH
Position: 152-166
Source molecule (GenBank): P22561.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S P26
Chemical type: Peptide from protein
Sequence: PSYGHTPSHHAAQFP
Position: 158-172
Source molecule (GenBank): P22561.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S P27
Chemical type: Peptide from protein
Sequence: QFPNHSFKHEDPMGQ
Position: 170-184
Source molecule (GenBank): P22561.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S P28
Chemical type: Peptide from protein
Sequence: FKHEDPMGQQGSLGE
Position: 176-190
Source molecule (GenBank): P22561.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S P29
Chemical type: Peptide from protein
Sequence: MGQQGSLGEQQYSVP
Position: 182-196
Source molecule (GenBank): P22561.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S P30
Chemical type: Peptide from protein
Sequence: TDSCTGSQALLLRTP
Position: 206-220
Source molecule (GenBank): P22561.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P31
Chemical type: Peptide, no natural source
Sequence: ATRVHSGSDVRDLNA
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P32
Chemical type: Peptide, no natural source
Sequence: GSDVRDLNALLPAVP
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P33
Chemical type: Peptide, no natural source
Sequence: LNALLPAVPSLPGGG
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P34
Chemical type: Peptide, no natural source
Sequence: AVPSLPGGGGCALPV
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P35
Chemical type: Peptide, no natural source
Sequence: AQWAPVLDFAPPAAP
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P36
Chemical type: Peptide, no natural source
Sequence: LDFAPPAAPYGSLGG
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P37
Chemical type: Peptide, no natural source
Sequence: AAPYGSLGGPHSFIK
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P38
Chemical type: Peptide, no natural source
Sequence: LGGPHSFIKQEPSWG
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P39
Chemical type: Peptide, no natural source
Sequence: FIKQEPSWGGADPHE
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P42
Chemical type: Peptide, no natural source
Sequence: TGTAGACRYGPFGAP
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P43
Chemical type: Peptide, no natural source
Sequence: CRYGPFGAPPPSQAP
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P44
Chemical type: Peptide, no natural source
Sequence: GAPPPSQAPSGQARM
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P45
Chemical type: Peptide, no natural source
Sequence: QAPSGQARMFPNAPY
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P46
Chemical type: Peptide, no natural source
Sequence: ARMFPNAPYLPNCLE
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P47
Chemical type: Peptide, no natural source
Sequence: AIRNQGYSTVAFDGT
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P48
Chemical type: Peptide, no natural source
Sequence: YSTVAFDGTPSYGHT
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P49
Chemical type: Peptide, no natural source
Sequence: HTPTDSCTGSQALL
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P50
Chemical type: Peptide, no natural source
Sequence: CTGSQALLLRTPYNS
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P51
Chemical type: Peptide, no natural source
Sequence: LLLRTPYNSDNLYQM
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P52
Chemical type: Peptide, no natural source
Sequence: YNSDNLYQMTSQLEC
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P53
Chemical type: Peptide, no natural source
Sequence: NQMNLGSTLKGHATG
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P54
Chemical type: Peptide, no natural source
Sequence: STLKGHATGYESDNH
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P55
Chemical type: Peptide, no natural source
Sequence: DNHTTPMLYSCGAQY
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P56
Chemical type: Peptide, no natural source
Sequence: ATGYESDNHTTPMLY
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P57
Chemical type: Peptide, no natural source
Sequence: MLYSCGAQYRIHTHG
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P59
Chemical type: Peptide, no natural source
Sequence: RVPGVAPTIVRSASE
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P60
Chemical type: Peptide, no natural source
Sequence: PTIVRSASETNEKRP
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S P61
Chemical type: Peptide from protein
Sequence: AGACRYGPFGPPPPS
Position: 104-118
Source molecule (GenBank): P22561.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S P62
Chemical type: Peptide from protein
Sequence: SGQARMFPNAPYLPS
Position: 122-136
Source molecule (GenBank): P22561.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S P63
Chemical type: Peptide from protein
Sequence: SQPTIRNQGYSTVTF
Position: 140-154
Source molecule (GenBank): P22561.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P13
Chemical type: Peptide from protein
Sequence: HAAQFPNHSFKHEDP
Position: 235-249
Source molecule (GenBank): F6Y2T6.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P14
Chemical type: Peptide from protein
Sequence: NHSFKHEDPMGQQGS
Position: 241-255
Source molecule (GenBank): F6Y2T6.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P15
Chemical type: Peptide from protein
Sequence: EDPMGQQGSLGEQQY
Position: 247-261
Source molecule (GenBank): F6Y2T6.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WT1-S cons P41
Chemical type: Peptide from protein
Sequence: HFSGQFTGTAGACRY
Position: 163-177
Source molecule (GenBank): F6Y2T6.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo