Reference: Isamu Z Hartman; AeRyon Kim; Robert J Cotter; Kimberly Walter; Sarat K Dalai; Tatiana Boronina; Wendell Griffith; David E Lanar; Robert Schwenk; Urszula Krzych; Robert N Cole; Scheherazade Sadegh-Nasseri. "A reductionist cell-free major histocompatibility complex class II antigen processing system identifies immunodominant epitopes." Nature medicine (2010).

Epitope: HA<sub>306-318</sub>
Chemical type: Peptide from protein
Sequence: PKYVKQNTLKLAT
Position: 306-318
Source molecule (GenBank): Q8V285.1
Assay category: TCell
Result: Positive-High
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: HA<sub>298-317</sub>
Chemical type: Peptide from protein
Sequence: INSSLPYQNIHPVTIGECPK
Position: 302-321
Source molecule (GenBank): P03452.2
Assay category: TCell
Result: Positive-Low
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: HA<sub>298-317</sub>
Chemical type: Peptide from protein
Sequence: INSSLPYQNIHPVTIGECPK
Position: 302-321
Source molecule (GenBank): P03452.2
Assay category: MhcLigandElution
Result: Positive

Epitope: CII<sub>273-305</sub> 4OH
Chemical type: Peptide from protein
Sequence: QTGEPGIAGFKGEQGPKGEPGPAGVQGAPGPAG
Position: 269-301
Source molecule (GenBank): P02459.3
Assay category: MhcLigandElution
Result: Positive

Epitope: CII<sub>280-294</sub>
Chemical type: Peptide from protein
Sequence: AGFKGEQGPKGEPGP
Position: 276-290
Source molecule (GenBank): P02459.3
Assay category: TCell
Result: Positive
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: HA<sub>259-274</sub>
Chemical type: Peptide from protein
Sequence: SNGNFIAPEYAYKIVK
Position: 259-274
Source molecule (GenBank): AAT73274.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: HA<sub>259-274</sub>
Chemical type: Peptide from protein
Sequence: SNGNFIAPEYAYKIVK
Position: 259-274
Source molecule (GenBank): AAT73274.1
Assay category: TCell
Result: Positive
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: HA<sub>259-274</sub>
Chemical type: Peptide from protein
Sequence: SNGNFIAPEYAYKIVK
Position: 259-274
Source molecule (GenBank): AAT73274.1
Assay category: TCell
Result: Positive
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: HA<sub>259-274</sub>
Chemical type: Peptide from protein
Sequence: SNGNFIAPEYAYKIVK
Position: 259-274
Source molecule (GenBank): AAT73274.1
Assay category: TCell
Result: Positive
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: HA<sub>259-274</sub>
Chemical type: Peptide from protein
Sequence: SNGNFIAPEYAYKIVK
Position: 259-274
Source molecule (GenBank): AAT73274.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Short Term Restimulated

Epitope: HA<sub>259-274</sub>
Chemical type: Peptide from protein
Sequence: SNGNFIAPEYAYKIVK
Position: 259-274
Source molecule (GenBank): AAT73274.1
Assay category: MhcLigandElution
Result: Positive

Epitope: LSA<sub>325-338</sub>
Chemical type: Peptide from protein
Sequence: YDNFQDEENIGIYK
Position: 162-175
Source molecule (GenBank): AAB59230.1
Assay category: MhcLigandElution
Result: Positive

Epitope: LSA<sub>197-226</sub>
Chemical type: Peptide from protein
Sequence: LPSENERGYYIPHQSSLPQDNRGNSRDSKE
Position: 34-63
Source molecule (GenBank): AAB59230.1
Assay category: MhcLigandElution
Result: Positive

Epitope: LSA<sub>323-337</sub>
Chemical type: Peptide from protein
Sequence: VQYDNFQDEENIGIY
Position: 160-174
Source molecule (GenBank): AAB59230.1
Assay category: TCell
Result: Negative
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: LSA<sub>323-337</sub>
Chemical type: Peptide from protein
Sequence: VQYDNFQDEENIGIY
Position: 160-174
Source molecule (GenBank): AAB59230.1
Assay category: TCell
Result: Negative
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: LSA<sub>323-337</sub>
Chemical type: Peptide from protein
Sequence: VQYDNFQDEENIGIY
Position: 160-174
Source molecule (GenBank): AAB59230.1
Assay category: TCell
Result: Negative
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: LSA<sub>429-443</sub>
Chemical type: Peptide from protein
Sequence: VDELSEDITKYFMKL
Position: 266-280
Source molecule (GenBank): AAB59230.1
Assay category: TCell
Result: Negative
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: LSA<sub>429-443</sub>
Chemical type: Peptide from protein
Sequence: VDELSEDITKYFMKL
Position: 266-280
Source molecule (GenBank): AAB59230.1
Assay category: TCell
Result: Negative
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: LSA<sub>429-443</sub>
Chemical type: Peptide from protein
Sequence: VDELSEDITKYFMKL
Position: 266-280
Source molecule (GenBank): AAB59230.1
Assay category: TCell
Result: Negative
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: CII<sub>275-305</sub> 4OH
Chemical type: Peptide from protein
Sequence: GEPGIAGFKGEQGPKGEPGPAGVQGAPGPAG
Position: 271-301
Source molecule (GenBank): P02459.3
Assay category: MhcLigandElution
Result: Positive

Epitope: HA<sub>259-278</sub>
Chemical type: Peptide from protein
Sequence: SNGNFIAPEYAYKIVKKGDS
Position: 259-278
Source molecule (GenBank): AAT73274.1
Assay category: MhcLigandElution
Result: Positive

Epitope: LSA<sub>325-339</sub>
Chemical type: Peptide from protein
Sequence: YDNFQDEENIGIYKE
Position: 162-176
Source molecule (GenBank): AAB59230.1
Assay category: MhcLigandElution
Result: Positive

Epitope: HA (306-318)-containing peptide
Chemical type: Peptide, no natural source
Sequence: GACPKYVKQNTLKLATGMRK
Assay category: MhcLigandElution
Result: Positive

Epitope: HA (306-318)-containing peptide
Chemical type: Peptide, no natural source
Sequence: SRGACPKYVKQNTLKLATGMRK
Assay category: MhcLigandElution
Result: Positive

Epitope: HA<sub>297-317</sub>
Chemical type: Peptide from protein
Sequence: AINSSLPYQNIHPVTIGECPK
Position: 301-321
Source molecule (GenBank): P03452.2
Assay category: MhcLigandElution
Result: Positive

Epitope: LSA<sub>434-453</sub>
Chemical type: Peptide, no natural source
Sequence: EDITKYFMKLGGSGSPHHHH
Assay category: MhcLigandElution
Result: Positive

Epitope: LSA<sub>436-449</sub>
Chemical type: Peptide, no natural source
Sequence: ITKYFMKLGGSGSP
Assay category: TCell
Result: Positive
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: LSA<sub>436-449</sub>
Chemical type: Peptide, no natural source
Sequence: ITKYFMKLGGSGSP
Assay category: TCell
Result: Positive
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: LSA<sub>436-449</sub>
Chemical type: Peptide, no natural source
Sequence: ITKYFMKLGGSGSP
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: LSA<sub>436-449</sub>
Chemical type: Peptide, no natural source
Sequence: ITKYFMKLGGSGSP
Assay category: TCell
Result: Positive
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: CL<sub>13-23</sub>
Chemical type: Peptide from protein
Sequence: FAALNAQHVLA
Position: 13-23
Source molecule (GenBank): NP_054032.1
Assay category: MhcLigandElution
Result: Positive