Reference: James McAuliffe; Silvia Panetti; Emily Steffke; Amanda Wicki; Vinnycius Pereira-Almeida; Laurine Noblecourt; Yushu Hu; Shi Yu William Guo; Julie Lesenfants; Ramiro Andrei Ramirez-Valdez; Vineethkrishna Chandrasekar; Maryam Ahmad; Vincent Stroobant; Nathalie Vigneron; Benoit J Van den Eynde; Carol Sze Ki Leung. "Novel H-2D<sup>b</sup>-restricted CD8 epitope derived from mouse MAGE-type antigen P1A mediates antitumor immunity in C57BL/6 mice." Journal for immunotherapy of cancer (2024).

Epitope: PIA 1-15
Chemical type: Peptide from protein
Sequence: MSDNKKPDKAHSGSG
Position: 1-15
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 5-19
Chemical type: Peptide from protein
Sequence: KKPDKAHSGSGGDGD
Position: 5-19
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 9-23
Chemical type: Peptide from protein
Sequence: KAHSGSGGDGDGNRC
Position: 9-23
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 13-27
Chemical type: Peptide from protein
Sequence: GSGGDGDGNRCNLLH
Position: 13-27
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 17-31
Chemical type: Peptide from protein
Sequence: DGDGNRCNLLHRYSL
Position: 17-31
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 21-35
Chemical type: Peptide from protein
Sequence: NRCNLLHRYSLEEIL
Position: 21-35
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 25-39
Chemical type: Peptide from protein
Sequence: LLHRYSLEEILPYLG
Position: 25-39
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 29-43
Chemical type: Peptide from protein
Sequence: YSLEEILPYLGWLVF
Position: 29-43
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 53-67
Chemical type: Peptide from protein
Sequence: LQMFIDALYEEQYER
Position: 53-67
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 57-71
Chemical type: Peptide from protein
Sequence: IDALYEEQYERDVAW
Position: 57-71
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 61-75
Chemical type: Peptide from protein
Sequence: YEEQYERDVAWIARQ
Position: 61-75
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 65-79
Chemical type: Peptide from protein
Sequence: YERDVAWIARQSKRM
Position: 65-79
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 69-83
Chemical type: Peptide from protein
Sequence: VAWIARQSKRMSSVD
Position: 69-83
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 73-87
Chemical type: Peptide from protein
Sequence: ARQSKRMSSVDEDED
Position: 73-87
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 77-91
Chemical type: Peptide from protein
Sequence: KRMSSVDEDEDDEDD
Position: 77-91
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 81-95
Chemical type: Peptide from protein
Sequence: SVDEDEDDEDDEDDY
Position: 81-95
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 85-99
Chemical type: Peptide from protein
Sequence: DEDDEDDEDDYYDDE
Position: 85-99
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 89-103
Chemical type: Peptide from protein
Sequence: EDDEDDYYDDEDDDD
Position: 89-103
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 97-111
Chemical type: Peptide from protein
Sequence: DDEDDDDDAFYDDED
Position: 97-111
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 101-115
Chemical type: Peptide from protein
Sequence: DDDDAFYDDEDDEEE
Position: 101-115
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 105-119
Chemical type: Peptide from protein
Sequence: AFYDDEDDEEEELEN
Position: 105-119
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 109-123
Chemical type: Peptide from protein
Sequence: DEDDEEEELENLMDD
Position: 109-123
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 113-127
Chemical type: Peptide from protein
Sequence: EEEELENLMDDESED
Position: 113-127
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 117-131
Chemical type: Peptide from protein
Sequence: LENLMDDESEDEAEE
Position: 117-131
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 121-135
Chemical type: Peptide from protein
Sequence: MDDESEDEAEEEMSV
Position: 121-135
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 125-139
Chemical type: Peptide from protein
Sequence: SEDEAEEEMSVEMGA
Position: 125-139
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 129-143
Chemical type: Peptide from protein
Sequence: AEEEMSVEMGAGAEE
Position: 129-143
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 133-147
Chemical type: Peptide from protein
Sequence: MSVEMGAGAEEMGAG
Position: 133-147
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 137-151
Chemical type: Peptide from protein
Sequence: MGAGAEEMGAGANCA
Position: 137-151
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 141-155
Chemical type: Peptide from protein
Sequence: AEEMGAGANCACVPG
Position: 141-155
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 145-159
Chemical type: Peptide from protein
Sequence: GAGANCACVPGHHLR
Position: 145-159
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 149-163
Chemical type: Peptide from protein
Sequence: NCACVPGHHLRKNEV
Position: 149-163
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 153-167
Chemical type: Peptide from protein
Sequence: VPGHHLRKNEVKCRM
Position: 153-167
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 165-179
Chemical type: Peptide from protein
Sequence: CRMIYFFHDPNFLVS
Position: 165-179
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 169-183
Chemical type: Peptide from protein
Sequence: YFFHDPNFLVSIPVN
Position: 169-183
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 173-187
Chemical type: Peptide from protein
Sequence: DPNFLVSIPVNPKEQ
Position: 173-187
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 177-191
Chemical type: Peptide from protein
Sequence: LVSIPVNPKEQMECR
Position: 177-191
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 181-195
Chemical type: Peptide from protein
Sequence: PVNPKEQMECRCENA
Position: 181-195
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 185-199
Chemical type: Peptide from protein
Sequence: KEQMECRCENADEEV
Position: 185-199
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 189-203
Chemical type: Peptide from protein
Sequence: ECRCENADEEVAMEE
Position: 189-203
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 193-207
Chemical type: Peptide from protein
Sequence: ENADEEVAMEEEEEE
Position: 193-207
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 197-211
Chemical type: Peptide from protein
Sequence: EEVAMEEEEEEEEEE
Position: 197-211
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 201-215
Chemical type: Peptide from protein
Sequence: MEEEEEEEEEEEEEE
Position: 201-215
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 205-219
Chemical type: Peptide from protein
Sequence: EEEEEEEEEEEMGNP
Position: 205-219
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PIA 209-223
Chemical type: Peptide from protein
Sequence: EEEEEEEMGNPDGFS
Position: 209-223
Source molecule (GenBank): NP_035765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo