Reference: Kwok-Ho Lam; Jacqueline M Tremblay; Edwin Vazquez-Cintron; Kay Perry; Celinia Ondeck; Robert P Webb; Patrick M McNutt; Charles B Shoemaker; Rongsheng Jin. "Structural Insights into Rational Design of Single-Domain Antibody-Based Antitoxins against Botulinum Neurotoxins." Cell reports (2020).

Epitope: Epitope of VHH ciA-H7 on BoNT/A
Chemical type: Discontinuous protein residues
Discontinuous residues: S121, T122, I123, D124, E126, E171, V172, N174, R177, N238, N240, R241, L277, E281, L284, Y285, N288, K289, K291, D292, T296, D474, K477
Source molecule (GenBank): Q7B8V4
Assay category: BCell
Result: Positive
Quantitative measurement: 2.02

Epitope: Epitope of VHH ciA-H7 on BoNT/A
Chemical type: Discontinuous protein residues
Discontinuous residues: S121, T122, I123, D124, E126, E171, V172, N174, R177, N238, N240, R241, L277, E281, L284, Y285, N288, K289, K291, D292, T296, D474, K477
Source molecule (GenBank): Q7B8V4
Assay category: BCell
Result: Positive

Epitope: Epitope of VHH ciA-H7 on BoNT/A
Chemical type: Discontinuous protein residues
Discontinuous residues: S121, T122, I123, D124, E126, E171, V172, N174, R177, N238, N240, R241, L277, E281, L284, Y285, N288, K289, K291, D292, T296, D474, K477
Source molecule (GenBank): Q7B8V4
Assay category: BCell
Result: Positive
Quantitative measurement: 2.20001

Epitope: Epitope of VHH ciA-D12 on BoNT/A
Chemical type: Discontinuous protein residues
Discontinuous residues: V4, K6, Q7, T92, K93, E96, K381, V382, T385, I386, Y387, R393, I408, M411, N412
Source molecule (GenBank): Q7B8V4
Assay category: BCell
Result: Positive
Quantitative measurement: 2.20001

Epitope: Epitope of VHH ciA-D12 on BoNT/A
Chemical type: Discontinuous protein residues
Discontinuous residues: V4, K6, Q7, T92, K93, E96, K381, V382, T385, I386, Y387, R393, I408, M411, N412
Source molecule (GenBank): Q7B8V4
Assay category: BCell
Result: Positive

Epitope: Epitope of VHH ciA-B5 on BoNT/A
Chemical type: Discontinuous protein residues
Discontinuous residues: L207, N394, T395, N396, N400, N402, A600, A601, L604, V607, E608, Q609, V611, Y612, D616, E758, N760, N761, I762, N763, F764, N765, D768, K772
Source molecule (GenBank): Q7B8V4
Assay category: BCell
Result: Positive
Quantitative measurement: 2.20001

Epitope: Epitope of VHH ciA-B5 on BoNT/A
Chemical type: Discontinuous protein residues
Discontinuous residues: L207, N394, T395, N396, N400, N402, A600, A601, L604, V607, E608, Q609, V611, Y612, D616, E758, N760, N761, I762, N763, F764, N765, D768, K772
Source molecule (GenBank): Q7B8V4
Assay category: BCell
Result: Positive

Epitope: Epitope of VHH JLI-H11 on BoNT/B
Chemical type: Discontinuous protein residues
Discontinuous residues: N1095, E1097, K1144, R1148, D1170, F1172, L1174, N1175, Q1176, E1177, P1197, I1198, S1199, D1200
Source molecule (GenBank): P10844.3
Assay category: BCell
Result: Positive
Quantitative measurement: 2.32006

Epitope: Epitope of VHH JLI-H11 on BoNT/B
Chemical type: Discontinuous protein residues
Discontinuous residues: N1095, E1097, K1144, R1148, D1170, F1172, L1174, N1175, Q1176, E1177, P1197, I1198, S1199, D1200
Source molecule (GenBank): P10844.3
Assay category: BCell
Result: Positive

Epitope: Epitope of VHH JLI-H11 on BoNT/B
Chemical type: Discontinuous protein residues
Discontinuous residues: N1095, E1097, K1144, R1148, D1170, F1172, L1174, N1175, Q1176, E1177, P1197, I1198, S1199, D1200
Source molecule (GenBank): P10844.3
Assay category: BCell
Result: Positive

Epitope: Epitope of VHH JLI-H11 on BoNT/B
Chemical type: Discontinuous protein residues
Discontinuous residues: N1095, E1097, K1144, R1148, D1170, F1172, L1174, N1175, Q1176, E1177, P1197, I1198, S1199, D1200
Source molecule (GenBank): P10844.3
Assay category: BCell
Result: Positive
Quantitative measurement: 10.0

Epitope: Epitope of VHH JLO-G11 on BoNT/B
Chemical type: Discontinuous protein residues
Discontinuous residues: K1113, D1115, S1116, P1117, V1118, Y1183, Y1185, F1186, K1187, K1188, E1191, K1192, L1193, F1194, D1202, E1203, Y1244, E1245, S1246, G1247, I1248, Y1256
Source molecule (GenBank): P10844.3
Assay category: BCell
Result: Positive
Quantitative measurement: 3.18424

Epitope: Epitope of VHH JLO-G11 on BoNT/B
Chemical type: Discontinuous protein residues
Discontinuous residues: K1113, D1115, S1116, P1117, V1118, Y1183, Y1185, F1186, K1187, K1188, E1191, K1192, L1193, F1194, D1202, E1203, Y1244, E1245, S1246, G1247, I1248, Y1256
Source molecule (GenBank): P10844.3
Assay category: BCell
Result: Positive
Quantitative measurement: 0.5

Epitope: Epitope of VHH JLO-G11 on BoNT/B
Chemical type: Discontinuous protein residues
Discontinuous residues: K1113, D1115, S1116, P1117, V1118, Y1183, Y1185, F1186, K1187, K1188, E1191, K1192, L1193, F1194, D1202, E1203, Y1244, E1245, S1246, G1247, I1248, Y1256
Source molecule (GenBank): P10844.3
Assay category: BCell
Result: Positive