Reference: Bennett J Davenport; Thomas E Morrison; Ross M Kedl; Jared Klarquist. "Conserved and Novel Mouse CD8 T Cell Epitopes within SARS-CoV-2 Spike Receptor Binding Domain Protein Identified following Subunit Vaccination." Journal of immunology (Baltimore, Md. : 1950) (2021).

Epitope: CoV-2 S 1-14, 319
Chemical type: Peptide, no natural source
Sequence: MFVFLVLLPLVSSQR
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 1-14, 319
Chemical type: Peptide, no natural source
Sequence: MFVFLVLLPLVSSQR
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 1-14, 319
Chemical type: Peptide, no natural source
Sequence: MFVFLVLLPLVSSQR
Assay category: MhcBinding
Result: Negative

Epitope: CoV-2 S 1-14, 319
Chemical type: Peptide, no natural source
Sequence: MFVFLVLLPLVSSQR
Assay category: MhcBinding
Result: Positive-Low

Epitope: CoV-2 S 5-14, 319-323
Chemical type: Peptide, no natural source
Sequence: LVLLPLVSSQRVQPT
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 9-14, 319-327
Chemical type: Peptide, no natural source
Sequence: PLVSSQRVQPTESIV
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 14, 323-331
Chemical type: Peptide, no natural source
Sequence: SQRVQPTESIVRFPN
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 325-339
Chemical type: Peptide from protein
Sequence: SIVRFPNITNLCPFG
Position: 325-339
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 329-343
Chemical type: Peptide from protein
Sequence: FPNITNLCPFGEVFN
Position: 329-343
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Positive-Low
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 333-347
Chemical type: Peptide from protein
Sequence: TNLCPFGEVFNATRF
Position: 333-347
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 337-351
Chemical type: Peptide from protein
Sequence: PFGEVFNATRFASVY
Position: 337-351
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 337-351
Chemical type: Peptide from protein
Sequence: PFGEVFNATRFASVY
Position: 337-351
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 337-351
Chemical type: Peptide from protein
Sequence: PFGEVFNATRFASVY
Position: 337-351
Source molecule (GenBank): QHD43416.1
Assay category: MhcBinding
Result: Negative

Epitope: CoV-2 S 337-351
Chemical type: Peptide from protein
Sequence: PFGEVFNATRFASVY
Position: 337-351
Source molecule (GenBank): QHD43416.1
Assay category: MhcBinding
Result: Negative

Epitope: CoV-2 S 341-355
Chemical type: Peptide from protein
Sequence: VFNATRFASVYAWNR
Position: 341-355
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 345-359
Chemical type: Peptide from protein
Sequence: TRFASVYAWNRKRIS
Position: 345-359
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 349-363
Chemical type: Peptide from protein
Sequence: SVYAWNRKRISNCVA
Position: 349-363
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 357-371
Chemical type: Peptide from protein
Sequence: RISNCVADYSVLYNS
Position: 357-371
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 361-375
Chemical type: Peptide from protein
Sequence: CVADYSVLYNSASFS
Position: 361-375
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 365-379
Chemical type: Peptide from protein
Sequence: YSVLYNSASFSTFKC
Position: 365-379
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 369-383
Chemical type: Peptide from protein
Sequence: YNSASFSTFKCYGVS
Position: 369-383
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 373-387
Chemical type: Peptide from protein
Sequence: SFSTFKCYGVSPTKL
Position: 373-387
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 377-391
Chemical type: Peptide from protein
Sequence: FKCYGVSPTKLNDLC
Position: 377-391
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 381-395
Chemical type: Peptide from protein
Sequence: GVSPTKLNDLCFTNV
Position: 381-395
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 385-399
Chemical type: Peptide from protein
Sequence: TKLNDLCFTNVYADS
Position: 385-399
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 389-403
Chemical type: Peptide from protein
Sequence: DLCFTNVYADSFVIR
Position: 389-403
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Positive-Low
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 393-407
Chemical type: Peptide from protein
Sequence: TNVYADSFVIRGDEV
Position: 393-407
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 397-411
Chemical type: Peptide from protein
Sequence: ADSFVIRGDEVRQIA
Position: 397-411
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 401-415
Chemical type: Peptide from protein
Sequence: VIRGDEVRQIAPGQT
Position: 401-415
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 401-415
Chemical type: Peptide from protein
Sequence: VIRGDEVRQIAPGQT
Position: 401-415
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 401-415
Chemical type: Peptide from protein
Sequence: VIRGDEVRQIAPGQT
Position: 401-415
Source molecule (GenBank): QHD43416.1
Assay category: MhcBinding
Result: Negative

Epitope: CoV-2 S 401-415
Chemical type: Peptide from protein
Sequence: VIRGDEVRQIAPGQT
Position: 401-415
Source molecule (GenBank): QHD43416.1
Assay category: MhcBinding
Result: Negative

Epitope: CoV-2 S 405-419
Chemical type: Peptide from protein
Sequence: DEVRQIAPGQTGKIA
Position: 405-419
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 409-423
Chemical type: Peptide from protein
Sequence: QIAPGQTGKIADYNY
Position: 409-423
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 417-431
Chemical type: Peptide from protein
Sequence: KIADYNYKLPDDFTG
Position: 417-431
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 421-435
Chemical type: Peptide from protein
Sequence: YNYKLPDDFTGCVIA
Position: 421-435
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 425-439
Chemical type: Peptide from protein
Sequence: LPDDFTGCVIAWNSN
Position: 425-439
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 429-443
Chemical type: Peptide from protein
Sequence: FTGCVIAWNSNNLDS
Position: 429-443
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 433-447
Chemical type: Peptide from protein
Sequence: VIAWNSNNLDSKVGG
Position: 433-447
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 437-451
Chemical type: Peptide from protein
Sequence: NSNNLDSKVGGNYNY
Position: 437-451
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 441-455
Chemical type: Peptide from protein
Sequence: LDSKVGGNYNYLYRL
Position: 441-455
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 445-459
Chemical type: Peptide from protein
Sequence: VGGNYNYLYRLFRKS
Position: 445-459
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 449-463
Chemical type: Peptide from protein
Sequence: YNYLYRLFRKSNLKP
Position: 449-463
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 453-467
Chemical type: Peptide from protein
Sequence: YRLFRKSNLKPFERD
Position: 453-467
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 457-471
Chemical type: Peptide from protein
Sequence: RKSNLKPFERDISTE
Position: 457-471
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 461-475
Chemical type: Peptide from protein
Sequence: LKPFERDISTEIYQA
Position: 461-475
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 465-479
Chemical type: Peptide from protein
Sequence: ERDISTEIYQAGSTP
Position: 465-479
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 477-491
Chemical type: Peptide from protein
Sequence: STPCNGVEGFNCYFP
Position: 477-491
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 477-491
Chemical type: Peptide from protein
Sequence: STPCNGVEGFNCYFP
Position: 477-491
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 477-491
Chemical type: Peptide from protein
Sequence: STPCNGVEGFNCYFP
Position: 477-491
Source molecule (GenBank): QHD43416.1
Assay category: MhcBinding
Result: Negative

Epitope: CoV-2 S 477-491
Chemical type: Peptide from protein
Sequence: STPCNGVEGFNCYFP
Position: 477-491
Source molecule (GenBank): QHD43416.1
Assay category: MhcBinding
Result: Positive-Low

Epitope: CoV-2 S 481-495
Chemical type: Peptide from protein
Sequence: NGVEGFNCYFPLQSY
Position: 481-495
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 493-507
Chemical type: Peptide from protein
Sequence: QSYGFQPTNGVGYQP
Position: 493-507
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 501-515
Chemical type: Peptide from protein
Sequence: NGVGYQPYRVVVLSF
Position: 501-515
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 509-523
Chemical type: Peptide from protein
Sequence: RVVVLSFELLHAPAT
Position: 509-523
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 509-523
Chemical type: Peptide from protein
Sequence: RVVVLSFELLHAPAT
Position: 509-523
Source molecule (GenBank): QHD43416.1
Assay category: MhcBinding
Result: Positive

Epitope: CoV-2 S 509-523
Chemical type: Peptide from protein
Sequence: RVVVLSFELLHAPAT
Position: 509-523
Source molecule (GenBank): QHD43416.1
Assay category: MhcBinding
Result: Positive-Low

Epitope: CoV-2 S 513-527
Chemical type: Peptide from protein
Sequence: LSFELLHAPATVCGP
Position: 513-527
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 517-531
Chemical type: Peptide from protein
Sequence: LLHAPATVCGPKKST
Position: 517-531
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 511-518
Chemical type: Peptide from protein
Sequence: VVLSFELL
Position: 511-518
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 511-518
Chemical type: Peptide from protein
Sequence: VVLSFELL
Position: 511-518
Source molecule (GenBank): QHD43416.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 511-518
Chemical type: Peptide from protein
Sequence: VVLSFELL
Position: 511-518
Source molecule (GenBank): QHD43416.1
Assay category: MhcBinding
Result: Positive-High

Epitope: CoV-2 S 511-518
Chemical type: Peptide from protein
Sequence: VVLSFELL
Position: 511-518
Source molecule (GenBank): QHD43416.1
Assay category: MhcBinding
Result: Negative

Epitope: CoV-2 S 529-541 2xH
Chemical type: Peptide, no natural source
Sequence: KSTNLVKNKCVNFHH
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CoV-2 S 533-541 6xH
Chemical type: Peptide, no natural source
Sequence: LVKNKCVNFHHHHHH
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo