Reference: Chao Liang; Qianhui Xia; Jingming Zhou; Hongliang Liu; Yumei Chen; Yankai Liu; Peiyang Ding; Yanhua Qi; Aiping Wang. "Identification of potential SLA-I-restricted CTL epitopes within the M protein of porcine reproductive and respiratory syndrome virus." Veterinary microbiology (2021).

Epitope: M-1
Chemical type: Peptide from protein
Sequence: CNDSTAPQKVLLAFS
Position: 9-23
Source molecule (GenBank): ADD69929.1
Assay category: TCell
Result: Positive
Host: M 5 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: M-2
Chemical type: Peptide from protein
Sequence: LLAFSITYTPVMIYALKVSR
Position: 19-38
Source molecule (GenBank): ADD69929.1
Assay category: TCell
Result: Positive
Host: M 5 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: M-3
Chemical type: Peptide from protein
Sequence: ALKVSRGRLLGLLHL
Position: 33-47
Source molecule (GenBank): ADD69929.1
Assay category: TCell
Result: Positive
Host: M 5 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: M-4
Chemical type: Peptide from protein
Sequence: SRGRLLGLLHLLIFLNCAFT
Position: 37-56
Source molecule (GenBank): ADD69929.1
Assay category: TCell
Result: Negative
Host: M 5 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: M-6
Chemical type: Peptide from protein
Sequence: LWGVYSAIETWKFITSRCRL
Position: 82-101
Source molecule (GenBank): ADD69929.1
Assay category: TCell
Result: Positive
Host: M 5 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: M-7
Chemical type: Peptide from protein
Sequence: KFITSRCRLCLLGRK
Position: 93-107
Source molecule (GenBank): ADD69929.1
Assay category: TCell
Result: Positive
Host: M 5 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: M-8
Chemical type: Peptide from protein
Sequence: RLCLLGRKYILAPAHHVESA
Position: 100-119
Source molecule (GenBank): ADD69929.1
Assay category: TCell
Result: Positive
Host: M 5 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: M-9
Chemical type: Peptide from protein
Sequence: LVLGGRKAVKQGVVNLVKYA
Position: 154-173
Source molecule (GenBank): ADD69929.1
Assay category: TCell
Result: Positive
Host: M 5 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: M1-1
Chemical type: Peptide from protein
Sequence: STAPQKVLL
Position: 12-20
Source molecule (GenBank): ADD69929.1
Assay category: MhcBinding
Result: Positive

Epitope: M2-4
Chemical type: Peptide from protein
Sequence: ITYTPVMIY
Position: 24-32
Source molecule (GenBank): ADD69929.1
Assay category: MhcBinding
Result: Negative

Epitope: M2-4
Chemical type: Peptide from protein
Sequence: ITYTPVMIY
Position: 24-32
Source molecule (GenBank): ADD69929.1
Assay category: MhcBinding
Result: Negative

Epitope: M2-4
Chemical type: Peptide from protein
Sequence: ITYTPVMIY
Position: 24-32
Source molecule (GenBank): ADD69929.1
Assay category: MhcBinding
Result: Negative

Epitope: M2-4
Chemical type: Peptide from protein
Sequence: ITYTPVMIY
Position: 24-32
Source molecule (GenBank): ADD69929.1
Assay category: MhcBinding
Result: Negative

Epitope: M2-5
Chemical type: Peptide from protein
Sequence: FSITYTPVM
Position: 22-30
Source molecule (GenBank): ADD69929.1
Assay category: TCell
Result: Positive
Host: M 5 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: M2-5
Chemical type: Peptide from protein
Sequence: FSITYTPVM
Position: 22-30
Source molecule (GenBank): ADD69929.1
Assay category: MhcBinding
Result: Positive

Epitope: M2-5
Chemical type: Peptide from protein
Sequence: FSITYTPVM
Position: 22-30
Source molecule (GenBank): ADD69929.1
Assay category: MhcBinding
Result: Negative

Epitope: M2-6
Chemical type: Peptide from protein
Sequence: SITYTPVMIY
Position: 23-32
Source molecule (GenBank): ADD69929.1
Assay category: MhcBinding
Result: Negative

Epitope: M2-6
Chemical type: Peptide from protein
Sequence: SITYTPVMIY
Position: 23-32
Source molecule (GenBank): ADD69929.1
Assay category: MhcBinding
Result: Negative

Epitope: M2-6
Chemical type: Peptide from protein
Sequence: SITYTPVMIY
Position: 23-32
Source molecule (GenBank): ADD69929.1
Assay category: MhcBinding
Result: Negative

Epitope: M3-4
Chemical type: Peptide from protein
Sequence: GRLLGLLHL
Position: 39-47
Source molecule (GenBank): ADD69929.1
Assay category: MhcBinding
Result: Negative

Epitope: M6-1
Chemical type: Peptide from protein
Sequence: YSAIETWKF
Position: 86-94
Source molecule (GenBank): ADD69929.1
Assay category: TCell
Result: Positive
Host: M 5 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: M6-1
Chemical type: Peptide from protein
Sequence: YSAIETWKF
Position: 86-94
Source molecule (GenBank): ADD69929.1
Assay category: MhcBinding
Result: Positive

Epitope: M6-1
Chemical type: Peptide from protein
Sequence: YSAIETWKF
Position: 86-94
Source molecule (GenBank): ADD69929.1
Assay category: MhcBinding
Result: Negative

Epitope: M6-1
Chemical type: Peptide from protein
Sequence: YSAIETWKF
Position: 86-94
Source molecule (GenBank): ADD69929.1
Assay category: MhcBinding
Result: Negative

Epitope: M6-1
Chemical type: Peptide from protein
Sequence: YSAIETWKF
Position: 86-94
Source molecule (GenBank): ADD69929.1
Assay category: MhcBinding
Result: Negative

Epitope: M6-2
Chemical type: Peptide from protein
Sequence: SAIETWKFI
Position: 87-95
Source molecule (GenBank): ADD69929.1
Assay category: MhcBinding
Result: Negative

Epitope: M6-2
Chemical type: Peptide from protein
Sequence: SAIETWKFI
Position: 87-95
Source molecule (GenBank): ADD69929.1
Assay category: MhcBinding
Result: Negative

Epitope: M6-3
Chemical type: Peptide from protein
Sequence: KFITSRCRL
Position: 93-101
Source molecule (GenBank): ADD69929.1
Assay category: MhcBinding
Result: Negative

Epitope: M6-5
Chemical type: Peptide from protein
Sequence: GVYSAIETW
Position: 84-92
Source molecule (GenBank): ADD69929.1
Assay category: MhcBinding
Result: Negative

Epitope: M7-2
Chemical type: Peptide from protein
Sequence: KFITSRCRL
Position: 93-101
Source molecule (GenBank): ADD69929.1
Assay category: MhcBinding
Result: Negative

Epitope: M8-1
Chemical type: Peptide from protein
Sequence: RLCLLGRKY
Position: 100-108
Source molecule (GenBank): ADD69929.1
Assay category: MhcBinding
Result: Negative

Epitope: M9-1
Chemical type: Peptide from protein
Sequence: AVKQGVVNL
Position: 161-169
Source molecule (GenBank): ADD69929.1
Assay category: TCell
Result: Positive
Host: M 5 weeks
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: M9-1
Chemical type: Peptide from protein
Sequence: AVKQGVVNL
Position: 161-169
Source molecule (GenBank): ADD69929.1
Assay category: MhcBinding
Result: Negative

Epitope: M9-1
Chemical type: Peptide from protein
Sequence: AVKQGVVNL
Position: 161-169
Source molecule (GenBank): ADD69929.1
Assay category: MhcBinding
Result: Positive