Reference: Alison J Johnson; Steven C Kennedy; Cecilia S Lindestam Arlehamn; Michael F Goldberg; Neeraj K Saini; Jiayong Xu; Sinu Paul; Subray S Hegde; John S Blanchard; John Chan; William R Jacobs; Alessandro Sette; Steven A Porcelli. "Identification of Mycobacterial RplJ/L10 and RpsA/S1 Proteins as Novel Targets for CD4<sup>+</sup> T Cells." Infection and immunity (2017).

Epitope: RplJ TB 140-154
Chemical type: Peptide from protein
Sequence: MKGNLAKAAGLFNAP
Position: 140-154
Source molecule (GenBank): WP_009935553.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RplJ TB 140-154
Chemical type: Peptide from protein
Sequence: MKGNLAKAAGLFNAP
Position: 140-154
Source molecule (GenBank): WP_009935553.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 6953.0

Epitope: RplJ TB 140-154
Chemical type: Peptide from protein
Sequence: MKGNLAKAAGLFNAP
Position: 140-154
Source molecule (GenBank): WP_009935553.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 753.0

Epitope: RplJ TB 141-155
Chemical type: Peptide from protein
Sequence: KGNLAKAAGLFNAPA
Position: 141-155
Source molecule (GenBank): WP_009935553.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RplJ TB 141-155
Chemical type: Peptide from protein
Sequence: KGNLAKAAGLFNAPA
Position: 141-155
Source molecule (GenBank): WP_009935553.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 841.0

Epitope: RplJ TB 142-156
Chemical type: Peptide from protein
Sequence: GNLAKAAGLFNAPAS
Position: 142-156
Source molecule (GenBank): WP_009935553.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RplJ TB 142-156
Chemical type: Peptide from protein
Sequence: GNLAKAAGLFNAPAS
Position: 142-156
Source molecule (GenBank): WP_009935553.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 4151.0

Epitope: RplJ TB 143-157
Chemical type: Peptide from protein
Sequence: NLAKAAGLFNAPASQ
Position: 143-157
Source molecule (GenBank): WP_009935553.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 535.0

Epitope: RplJ TB 144-158
Chemical type: Peptide from protein
Sequence: LAKAAGLFNAPASQL
Position: 144-158
Source molecule (GenBank): WP_009935553.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 123.0

Epitope: RplJ TB 145-158
Chemical type: Peptide from protein
Sequence: AKAAGLFNAPASQL
Position: 145-158
Source molecule (GenBank): WP_009935553.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 64.0

Epitope: RpIJ TB 146-160
Chemical type: Peptide from protein
Sequence: KAAGLFNAPASQLAR
Position: 146-160
Source molecule (GenBank): WP_009935553.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ TB 146-160
Chemical type: Peptide from protein
Sequence: KAAGLFNAPASQLAR
Position: 146-160
Source molecule (GenBank): WP_009935553.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ TB 146-160
Chemical type: Peptide from protein
Sequence: KAAGLFNAPASQLAR
Position: 146-160
Source molecule (GenBank): WP_009935553.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ TB 146-160
Chemical type: Peptide from protein
Sequence: KAAGLFNAPASQLAR
Position: 146-160
Source molecule (GenBank): WP_009935553.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Cell Line / Clone

Epitope: RpIJ TB 146-160
Chemical type: Peptide from protein
Sequence: KAAGLFNAPASQLAR
Position: 146-160
Source molecule (GenBank): WP_009935553.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ TB 146-160
Chemical type: Peptide from protein
Sequence: KAAGLFNAPASQLAR
Position: 146-160
Source molecule (GenBank): WP_009935553.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ TB 146-160
Chemical type: Peptide from protein
Sequence: KAAGLFNAPASQLAR
Position: 146-160
Source molecule (GenBank): WP_009935553.1
Assay category: TCell
Result: Positive-Low
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ TB 146-160
Chemical type: Peptide from protein
Sequence: KAAGLFNAPASQLAR
Position: 146-160
Source molecule (GenBank): WP_009935553.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 21.0

Epitope: RplJ TB 147-161
Chemical type: Peptide from protein
Sequence: AAGLFNAPASQLARL
Position: 147-161
Source molecule (GenBank): WP_009935553.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 5.3

Epitope: RplJ TB 149-163
Chemical type: Peptide from protein
Sequence: GLFNAPASQLARLAA
Position: 149-163
Source molecule (GenBank): WP_009935553.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 18.0

Epitope: RplJ TB 150-164
Chemical type: Peptide from protein
Sequence: LFNAPASQLARLAAA
Position: 150-164
Source molecule (GenBank): WP_009935553.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 14.0

Epitope: RplJ TB 151-165
Chemical type: Peptide from protein
Sequence: FNAPASQLARLAAAL
Position: 151-165
Source molecule (GenBank): WP_009935553.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RplJ TB 151-165
Chemical type: Peptide from protein
Sequence: FNAPASQLARLAAAL
Position: 151-165
Source molecule (GenBank): WP_009935553.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 54.0

Epitope: RpIJ MS 1
Chemical type: Peptide from protein
Sequence: MKGNLSKAAGLFNAP
Position: 140-154
Source molecule (GenBank): WP_003892752.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ MS 1
Chemical type: Peptide from protein
Sequence: MKGNLSKAAGLFNAP
Position: 140-154
Source molecule (GenBank): WP_003892752.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 1176.0

Epitope: RpIJ MS 3
Chemical type: Peptide from protein
Sequence: GNLSKAAGLFNAPAS
Position: 142-156
Source molecule (GenBank): WP_003892752.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ MS 3
Chemical type: Peptide from protein
Sequence: GNLSKAAGLFNAPAS
Position: 142-156
Source molecule (GenBank): WP_003892752.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 8911.0

Epitope: RpIJ MS 4
Chemical type: Peptide from protein
Sequence: NLSKAAGLFNAPASQ
Position: 143-157
Source molecule (GenBank): WP_003892752.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 299.0

Epitope: RpIJ MS 5
Chemical type: Peptide from protein
Sequence: LSKAAGLFNAPASQV
Position: 144-158
Source molecule (GenBank): WP_003892752.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 395.0

Epitope: RpIJ MS 6
Chemical type: Peptide from protein
Sequence: SKAAGLFNAPASQVA
Position: 145-159
Source molecule (GenBank): WP_003892752.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 203.0

Epitope: RpIJ MS 7
Chemical type: Peptide from protein
Sequence: KAAGLFNAPASQVAR
Position: 146-160
Source molecule (GenBank): WP_003892752.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ MS 7
Chemical type: Peptide from protein
Sequence: KAAGLFNAPASQVAR
Position: 146-160
Source molecule (GenBank): WP_003892752.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 40.0

Epitope: RpIJ MS 8
Chemical type: Peptide from protein
Sequence: AAGLFNAPASQVARL
Position: 147-161
Source molecule (GenBank): WP_003892752.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 37.0

Epitope: RpIJ MS 9
Chemical type: Peptide from protein
Sequence: AGLFNAPASQVARLA
Position: 148-162
Source molecule (GenBank): WP_003892752.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 19.0

Epitope: RpIJ MS 10
Chemical type: Peptide from protein
Sequence: GLFNAPASQVARLAA
Position: 149-163
Source molecule (GenBank): WP_003892752.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 23.0

Epitope: RpIJ MS 11
Chemical type: Peptide from protein
Sequence: LFNAPASQVARLAAA
Position: 150-164
Source molecule (GenBank): WP_003892752.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 21.0

Epitope: RpIJ MS 12
Chemical type: Peptide from protein
Sequence: FNAPASQVARLAAAL
Position: 151-165
Source molecule (GenBank): WP_003892752.1
Assay category: TCell
Result: Positive-Low
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ MS 12
Chemical type: Peptide from protein
Sequence: FNAPASQVARLAAAL
Position: 151-165
Source molecule (GenBank): WP_003892752.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 36.0

Epitope: RpIJ MS 13
Chemical type: Peptide from protein
Sequence: NAPASQVARLAAALQ
Position: 152-166
Source molecule (GenBank): WP_003892752.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ MS 13
Chemical type: Peptide from protein
Sequence: NAPASQVARLAAALQ
Position: 152-166
Source molecule (GenBank): WP_003892752.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 642.0

Epitope: RpIJ TM 67-81
Chemical type: Peptide from protein
Sequence: SFTFTLKTPPAAKLL
Position: 67-81
Source molecule (GenBank): WP_003909267.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ TM  68-82
Chemical type: Peptide from protein
Sequence: GLDELFVGPTAIAFV
Position: 68-82
Source molecule (GenBank): WP_009935553.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ TM 30-44
Chemical type: Peptide from protein
Sequence: ETFEVTAAAPVAVAA
Position: 30-44
Source molecule (GenBank): CAB07109.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: EIV 221-235
Chemical type: Peptide from protein
Sequence: AAVKAAGAPVICETA
Position: 221-235
Source molecule (GenBank): NP_215184.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: acyl-CoA 11-25
Chemical type: Peptide from protein
Sequence: VPPLENYNPASSPVL
Position: 11-25
Source molecule (GenBank): WP_019256786.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: enoyl-CoA 23-37
Chemical type: Peptide from protein
Sequence: SRNAVNGPTAAALCA
Position: 23-37
Source molecule (GenBank): WP_015626821.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: enoyl-CoA 64-78
Chemical type: Peptide from protein
Sequence: ADLKAFGTPEANSVH
Position: 64-78
Source molecule (GenBank): WP_015626821.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Tmp 919-933
Chemical type: Peptide from protein
Sequence: LIVRSFMTPSIAALL
Position: 919-933
Source molecule (GenBank): WP_015631206.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: FR 387-401
Chemical type: Peptide from protein
Sequence: VRGKIAAGAPIAEVL
Position: 387-401
Source molecule (GenBank): WP_015629662.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: TetR 48 - 62
Chemical type: Peptide from protein
Sequence: RTLFRYYASKNAIPW
Position: 48-62
Source molecule (GenBank): NP_215205.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: DHN 223-237
Chemical type: Peptide from protein
Sequence: PHSTVSYVPSAAIVC
Position: 223-237
Source molecule (GenBank): CMN00729.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: DHN 293-307
Chemical type: Peptide from protein
Sequence: RKAFYGGSAAPLAVR
Position: 293-307
Source molecule (GenBank): CMN00729.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ TM 25-39
Chemical type: Peptide from protein
Sequence: PVTVVKAGPNVVTRI
Position: 25-39
Source molecule (GenBank): WP_015626836.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ TM 45-59
Chemical type: Peptide from protein
Sequence: ILRWAPQAASGPVAK
Position: 45-59
Source molecule (GenBank): P61181.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ TM  57-71
Chemical type: Peptide from protein
Sequence: VAKVIASAAANAQNN
Position: 57-71
Source molecule (GenBank): P61181.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ TM  104-118
Chemical type: Peptide from protein
Sequence: LEFALGYSHPVVIEA
Position: 104-118
Source molecule (GenBank): WP_015626848.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: AMDM 112-126
Chemical type: Peptide from protein
Sequence: DARAYRLPWPAGTVV
Position: 112-126
Source molecule (GenBank): WP_003909277.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ MS 150-158
Chemical type: Peptide from protein
Sequence: LFNAPASQV
Position: 150-158
Source molecule (GenBank): WP_003892752.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ MS 11-24
Chemical type: Peptide from protein
Sequence: ADIAEQFKASTATVV
Position: 11-25
Source molecule (GenBank): WP_003892752.1
Assay category: TCell
Result: Positive-Low
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ MS 16-30
Chemical type: Peptide from protein
Sequence: QFKASTATVVTEYRG
Position: 16-30
Source molecule (GenBank): WP_003892752.1
Assay category: TCell
Result: Positive-Low
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ MS 66-80
Chemical type: Peptide from protein
Sequence: IEGLDELFAGPTAIA
Position: 66-80
Source molecule (GenBank): WP_003892752.1
Assay category: TCell
Result: Positive-Low
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ MS 71-85
Chemical type: Peptide from protein
Sequence: ELFAGPTAWAFVKGE
Assay category: TCell
Result: Positive-Low
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ TB 76-90
Chemical type: Peptide from protein
Sequence: PTAIAFVTGEPVDAA
Position: 76-90
Source molecule (GenBank): WP_009935553.1
Assay category: TCell
Result: Positive-Low
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ TB 106-120
Chemical type: Peptide from protein
Sequence: KGGYMDGHPLTVAEV
Position: 106-120
Source molecule (GenBank): WP_009935553.1
Assay category: TCell
Result: Positive-Low
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ TB 136-150
Chemical type: Peptide from protein
Sequence: LAGAMKGNLAKAAGL
Position: 136-150
Source molecule (GenBank): WP_009935553.1
Assay category: TCell
Result: Positive-Low
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: rpsA TB 51-65
Chemical type: Peptide from protein
Sequence: VLLDIGYKTEGVIPA
Position: 51-65
Source molecule (GenBank): O06147.1
Assay category: TCell
Result: Positive-Low
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ MS 141-155
Chemical type: Peptide from protein
Sequence: DIGLRGFLPASLVEM
Position: 65-79
Source molecule (GenBank): AGZ16045.1
Assay category: TCell
Result: Positive-Low
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ MS 281-295
Chemical type: Peptide from protein
Sequence: EDPWRHFARTHAIGQ
Position: 205-219
Source molecule (GenBank): AGZ16045.1
Assay category: TCell
Result: Positive-Low
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ MS 431-445
Chemical type: Peptide from protein
Sequence: QMEKFAAAEAEAANA
Position: 431-445
Source molecule (GenBank): YP_888124.1
Assay category: TCell
Result: Positive-Low
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RpIJ MS 436-450
Chemical type: Peptide from protein
Sequence: AAAEAEAANAPVANG
Assay category: TCell
Result: Positive-Low
Host: F 6 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo