Reference: Jian Chen; Qi Wu; Pingar Yang; Hui-Chen Hsu; John D Mountz. "Determination of specific CD4 and CD8 T cell epitopes after AAV2- and AAV8-hF.IX gene therapy." Molecular therapy : the journal of the American Society of Gene Therapy (2006).

Epitope: AAV2 epitope (373-381)
Chemical type: Peptide from protein
Sequence: QYGSVSTNL
Position: 575-583
Source molecule (GenBank): AAC03780.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: mononuclear cell
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: AAV2 epitope (373-381)
Chemical type: Peptide from protein
Sequence: QYGSVSTNL
Position: 575-583
Source molecule (GenBank): AAC03780.1
Assay category: TCell
Result: Positive-Low
Host: F 6-8 weeks

Epitope: AAV2 epitope (470-478)
Chemical type: Peptide from protein
Sequence: QYSTGQVSV
Position: 672-680
Source molecule (GenBank): AAC03780.1
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: mononuclear cell
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: AAV2 epitope (470-478)
Chemical type: Peptide from protein
Sequence: QYSTGQVSV
Position: 672-680
Source molecule (GenBank): AAC03780.1
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: mononuclear cell
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: AAV8 epitope (50-58)
Chemical type: Peptide from protein
Sequence: KYLGPFNGL
Position: 51-59
Source molecule (GenBank): YP_077180.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks

Epitope: AAV8 epitope (50-58)
Chemical type: Peptide from protein
Sequence: KYLGPFNGL
Position: 51-59
Source molecule (GenBank): YP_077180.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: mononuclear cell
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: AAV8 epitope (50-58)
Chemical type: Peptide from protein
Sequence: KYLGPFNGL
Position: 51-59
Source molecule (GenBank): YP_077180.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: mononuclear cell
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: AAV8 epitope (577-585)
Chemical type: Peptide from protein
Sequence: EYGIVADNL
Position: 578-586
Source molecule (GenBank): YP_077180.1
Assay category: TCell
Result: Positive-Low
Host: F 6-8 weeks
Cell type: mononuclear cell
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: AAV8 epitope (577-585)
Chemical type: Peptide from protein
Sequence: EYGIVADNL
Position: 578-586
Source molecule (GenBank): YP_077180.1
Assay category: TCell
Result: Positive-Low
Host: F 6-8 weeks
Cell type: mononuclear cell
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: hF.IX epitope (311-319)
Chemical type: Peptide from protein
Sequence: KYNHDIALL
Position: 311-319
Source molecule (GenBank): P00740.2
Assay category: TCell
Result: Positive
Host: F 6-8 weeks

Epitope: hF.IX epitope (311-319)
Chemical type: Peptide from protein
Sequence: KYNHDIALL
Position: 311-319
Source molecule (GenBank): P00740.2
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: mononuclear cell
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: hF.IX epitope (311-319)
Chemical type: Peptide from protein
Sequence: KYNHDIALL
Position: 311-319
Source molecule (GenBank): P00740.2
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: mononuclear cell
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: hF.IX epitope (351-359)
Chemical type: Peptide from protein
Sequence: GYVSGWGRV
Position: 351-359
Source molecule (GenBank): P00740.2
Assay category: TCell
Result: Positive-Low
Host: F 6-8 weeks
Cell type: mononuclear cell
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: hF.IX epitope (351-359)
Chemical type: Peptide from protein
Sequence: GYVSGWGRV
Position: 351-359
Source molecule (GenBank): P00740.2
Assay category: TCell
Result: Positive-Low
Host: F 6-8 weeks
Cell type: mononuclear cell
Tissue: liver
Culture conditions: Direct Ex Vivo

Epitope: AAV2 epitope (336-350)
Chemical type: Peptide from protein
Sequence: GVLIFGKQGSEKTNV
Position: 336-350
Source molecule (GenBank): YP_680428.1
Assay category: TCell
Result: Positive-Low
Host: F 6-8 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: AAV2 epitope (336-350)
Chemical type: Peptide from protein
Sequence: GVLIFGKQGSEKTNV
Position: 336-350
Source molecule (GenBank): YP_680428.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: AAV8 epitope (50-64)
Chemical type: Peptide from protein
Sequence: YKYLGPFNGLDKGEP
Position: 50-64
Source molecule (GenBank): YP_077180.1
Assay category: TCell
Result: Positive-Low
Host: F 6-8 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: AAV8 epitope (50-64)
Chemical type: Peptide from protein
Sequence: YKYLGPFNGLDKGEP
Position: 50-64
Source molecule (GenBank): YP_077180.1
Assay category: TCell
Result: Positive-Low
Host: F 6-8 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: AAV8 epitope (126-140)
Chemical type: Peptide from protein
Sequence: LEPLGLVEEGAKTAP
Position: 126-140
Source molecule (GenBank): YP_077180.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: AAV8 epitope (126-140)
Chemical type: Peptide from protein
Sequence: LEPLGLVEEGAKTAP
Position: 126-140
Source molecule (GenBank): YP_077180.1
Assay category: TCell
Result: Positive-Low
Host: F 6-8 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: hF.IX epitope (312-326)
Chemical type: Peptide from protein
Sequence: YNHDIALLELDEPLV
Position: 312-326
Source molecule (GenBank): P00740.2
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: hF.IX epitope (312-326)
Chemical type: Peptide from protein
Sequence: YNHDIALLELDEPLV
Position: 312-326
Source molecule (GenBank): P00740.2
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: hF.IX epitope (108-122)
Chemical type: Peptide from protein
Sequence: CKDDINSYECWCPFG
Position: 108-122
Source molecule (GenBank): P00740.2
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: hF.IX epitope (108-122)
Chemical type: Peptide from protein
Sequence: CKDDINSYECWCPFG
Position: 108-122
Source molecule (GenBank): P00740.2
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: hF.IX epitope (328-342)
Chemical type: Peptide from protein
Sequence: NSYVTPICIADKEYT
Position: 328-342
Source molecule (GenBank): P00740.2
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: hF.IX epitope (328-342)
Chemical type: Peptide from protein
Sequence: NSYVTPICIADKEYT
Position: 328-342
Source molecule (GenBank): P00740.2
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: mononuclear cell
Tissue: spleen
Culture conditions: Direct Ex Vivo