Reference: Enayat Nikoopour; Rebecca Cheung; Stacey Bellemore; Olga Krougly; Edwin Lee-Chan; Mats Stridsberg; Bhagirath Singh. "Vasostatin-1 antigenic epitope mapping for induction of cellular and humoral immune responses to chromogranin A autoantigen in NOD mice." European journal of immunology (2014).

Epitope: ChgA 34–42
Chemical type: Peptide from protein
Sequence: KCVLEVISD
Position: 34-42
Source molecule (GenBank): P26339.1
Assay category: TCell
Result: Positive-Low
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: ChgA 34–42
Chemical type: Peptide from protein
Sequence: KCVLEVISD
Position: 34-42
Source molecule (GenBank): P26339.1
Assay category: TCell
Result: Positive
Host:  6-8 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: ChgA 34–42
Chemical type: Peptide from protein
Sequence: KCVLEVISD
Position: 34-42
Source molecule (GenBank): P26339.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 10000.0

Epitope: ChgA 33–42
Chemical type: Peptide from protein
Sequence: MKCVLEVISD
Position: 33-42
Source molecule (GenBank): P26339.1
Assay category: TCell
Result: Positive-Low
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: ChgA 33–42
Chemical type: Peptide from protein
Sequence: MKCVLEVISD
Position: 33-42
Source molecule (GenBank): P26339.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 8000.0

Epitope: ChgA 32–42
Chemical type: Peptide from protein
Sequence: VMKCVLEVISD
Position: 32-42
Source molecule (GenBank): P26339.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 7000.0

Epitope: ChgA 31–42
Chemical type: Peptide from protein
Sequence: KVMKCVLEVISD
Position: 31-42
Source molecule (GenBank): P26339.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: ChgA 31–42
Chemical type: Peptide from protein
Sequence: KVMKCVLEVISD
Position: 31-42
Source molecule (GenBank): P26339.1
Assay category: TCell
Result: Positive
Host:  6-8 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: ChgA 31–42
Chemical type: Peptide from protein
Sequence: KVMKCVLEVISD
Position: 31-42
Source molecule (GenBank): P26339.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 6000.0

Epitope: ChgA 30–42
Chemical type: Peptide from protein
Sequence: TKVMKCVLEVISD
Position: 30-42
Source molecule (GenBank): P26339.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 4000.0

Epitope: ChgA 29–42
Chemical type: Peptide from protein
Sequence: DTKVMKCVLEVISD
Position: 29-42
Source molecule (GenBank): P26339.1
Assay category: BCell
Result: Positive

Epitope: ChgA 29–42
Chemical type: Peptide from protein
Sequence: DTKVMKCVLEVISD
Position: 29-42
Source molecule (GenBank): P26339.1
Assay category: BCell
Result: Positive
Host:  6-8 weeks

Epitope: ChgA 29–42
Chemical type: Peptide from protein
Sequence: DTKVMKCVLEVISD
Position: 29-42
Source molecule (GenBank): P26339.1
Assay category: BCell
Result: Positive

Epitope: ChgA 29–42
Chemical type: Peptide from protein
Sequence: DTKVMKCVLEVISD
Position: 29-42
Source molecule (GenBank): P26339.1
Assay category: TCell
Result: Positive-High
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: ChgA 29–42
Chemical type: Peptide from protein
Sequence: DTKVMKCVLEVISD
Position: 29-42
Source molecule (GenBank): P26339.1
Assay category: TCell
Result: Positive-High
Host:  6-8 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: ChgA 29–42
Chemical type: Peptide from protein
Sequence: DTKVMKCVLEVISD
Position: 29-42
Source molecule (GenBank): P26339.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 2000.0

Epitope: ChgA 29–42 A34
Chemical type: Peptide, no natural source
Sequence: DTKVMACVLEVISD
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 3000.0

Epitope: ChgA 29–42 A37
Chemical type: Peptide, no natural source
Sequence: DTKVMKCVAEVISD
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 8000.0

Epitope: ChgA 29–42 A39
Chemical type: Peptide, no natural source
Sequence: DTKVMKCVLEAISD
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 7000.0

Epitope: ChgA 29–42 A40
Chemical type: Peptide, no natural source
Sequence: DTKVMKCVLEVASD
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 8000.0

Epitope: ChgA 29–42 A42
Chemical type: Peptide, no natural source
Sequence: DTKVMKCVLEVISA
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 10000.0

Epitope: ChgA 29–44
Chemical type: Peptide from protein
Sequence: DTKVMKCVLEVISDSL
Position: 29-44
Source molecule (GenBank): P26339.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 3000.0

Epitope: ChgA 31–44
Chemical type: Peptide from protein
Sequence: KVMKCVLEVISDSL
Position: 31-44
Source molecule (GenBank): P26339.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 3000.0

Epitope: ChgA 34–44
Chemical type: Peptide from protein
Sequence: KCVLEVISDSL
Position: 34-44
Source molecule (GenBank): P26339.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: ChgA 34–44
Chemical type: Peptide from protein
Sequence: KCVLEVISDSL
Position: 34-44
Source molecule (GenBank): P26339.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 10000.0

Epitope: ChgA 34–46
Chemical type: Peptide, no natural source
Sequence: KSVLEVISDSLSK
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 10000.0

Epitope: ChgA 29–42 R34
Chemical type: Peptide, no natural source
Sequence: DTKVMRCVLEVISD
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 1000.0

Epitope: PS3 mimotope
Chemical type: Peptide, no natural source
Sequence: SRLGLWVRME
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PS3 mimotope
Chemical type: Peptide, no natural source
Sequence: SRLGLWVRME
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 100.0

Epitope: INSULIN B9-23
Chemical type: Peptide from protein
Sequence: ALYLVCGER
Position: 38-46
Source molecule (GenBank): P01326.1
Assay category: MhcBinding
Result: Positive

Epitope: IIP
Chemical type: Peptide from protein
Sequence: YPNELLQEYNWE
Position: 137-148
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Negative

Epitope: ChgA 29–38
Chemical type: Peptide from protein
Sequence: DTKVMKCVLE
Position: 29-38
Source molecule (GenBank): P26339.1
Assay category: MhcBinding
Result: Positive

Epitope: ChgA 26–38
Chemical type: Peptide from protein
Sequence: TKGDTKVMKCVLE
Position: 26-38
Source molecule (GenBank): P26339.1
Assay category: MhcBinding
Result: Positive

Epitope: ChgA 17–28
Chemical type: Peptide from protein
Sequence: FALPVNSPMTKG
Position: 17-28
Source molecule (GenBank): P26339.1
Assay category: MhcBinding
Result: Negative

Epitope: PS3 A2
Chemical type: Peptide, no natural source
Sequence: SALGLWVRME
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 1000.0

Epitope: PS3 A5
Chemical type: Peptide, no natural source
Sequence: SRLGAWVRME
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 6000.0

Epitope: PS3 A7
Chemical type: Peptide, no natural source
Sequence: SRLGLWARME
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 100.0

Epitope: PS3 A8
Chemical type: Peptide, no natural source
Sequence: SRLGLWVAME
Assay category: MhcBinding
Result: Positive

Epitope: PS3 A10
Chemical type: Peptide, no natural source
Sequence: SRLGLWVRMA
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 2000.0

Epitope: PS3 S7D10
Chemical type: Peptide, no natural source
Sequence: SRLGLWSRMD
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 500.0

Epitope: SRLGL-WE-14
Chemical type: Peptide, no natural source
Sequence: SRLGLWVRMEQLAKELTAE
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 100.0

Epitope: WE-14 E6
Chemical type: Peptide, no natural source
Sequence: WSRMDELAKELTAE
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 10000.0

Epitope: WE-14 KR
Chemical type: Peptide from protein
Sequence: WSRMDQLAKELTAEKR
Position: 358-373
Source molecule (GenBank): P26339.1
Assay category: MhcBinding
Result: Positive

Epitope: QLAKELTAEKR
Chemical type: Peptide from protein
Sequence: QLAKELTAEKR
Position: 363-373
Source molecule (GenBank): P26339.1
Assay category: MhcBinding
Result: Positive

Epitope: ChgA 63–76
Chemical type: Peptide from protein
Sequence: DERILSILRHQNLL
Position: 63-76
Source molecule (GenBank): AAB53685.1
Assay category: BCell
Result: Positive-Low

Epitope: ChgA 1–17
Chemical type: Peptide from protein
Sequence: MRSAAVLALLLCAGQVT
Position: 1-17
Source molecule (GenBank): AAB53685.1
Assay category: BCell
Result: Negative

Epitope: ChgA 324–337
Chemical type: Peptide from protein
Sequence: ELEQEEERLSKEWE
Position: 324-337
Source molecule (GenBank): AAB53685.1
Assay category: BCell
Result: Negative