Reference: Yusheng Jia; Qingxin Wu; Yilin Li; Mulin Ma; Wei Song; Rongmao Chen; Yongxiu Yao; Venugopal Nair; Nianzhi Zhang; Ming Liao; Manman Dai. "Revealing novel and conservative T-cell epitopes with MHC B2 restriction on H9N2 avian influenza virus (AIV)." The Journal of biological chemistry (2024).

Epitope: WV9 (PB2 552-560)
Chemical type: Peptide from protein
Sequence: WIIRNWETV
Position: 552-560
Source molecule (GenBank): AGY14277.1
Assay category: TCell
Result: Positive
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WV9 (PB2 552-560)
Chemical type: Peptide from protein
Sequence: WIIRNWETV
Position: 552-560
Source molecule (GenBank): AGY14277.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 1.9

Epitope: WV9 (PB2 552-560)
Chemical type: Peptide from protein
Sequence: WIIRNWETV
Position: 552-560
Source molecule (GenBank): AGY14277.1
Assay category: MhcBinding
Result: Positive-Intermediate

Epitope: NP (182-190)
Chemical type: Peptide from protein
Sequence: AVKGIGTMV
Position: 182-190
Source molecule (GenBank): AGY14271.1
Assay category: TCell
Result: Positive
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP (182-190)
Chemical type: Peptide from protein
Sequence: AVKGIGTMV
Position: 182-190
Source molecule (GenBank): AGY14271.1
Assay category: MhcBinding
Result: Positive-Low

Epitope: NP (455-463)
Chemical type: Peptide from protein
Sequence: DVSFQGRGV
Position: 455-463
Source molecule (GenBank): AGY14271.1
Assay category: TCell
Result: Positive
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP (455-463)
Chemical type: Peptide from protein
Sequence: DVSFQGRGV
Position: 455-463
Source molecule (GenBank): AGY14271.1
Assay category: MhcBinding
Result: Positive-Low

Epitope: NS1 (98-106)
Chemical type: Peptide from protein
Sequence: MSRDWLMLI
Position: 98-106
Source molecule (GenBank): AGY14287.1
Assay category: TCell
Result: Positive
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS1 (98-106)
Chemical type: Peptide from protein
Sequence: MSRDWLMLI
Position: 98-106
Source molecule (GenBank): AGY14287.1
Assay category: MhcBinding
Result: Positive-Low

Epitope: PB2 (725-733)
Chemical type: Peptide from protein
Sequence: LIGQGDVVL
Position: 725-733
Source molecule (GenBank): AGY14277.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PB2 (210-218)
Chemical type: Peptide from protein
Sequence: ELVRKTRFL
Position: 210-218
Source molecule (GenBank): AGY14277.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PB2 (344-352)
Chemical type: Peptide from protein
Sequence: VLTGNLQTL
Position: 344-352
Source molecule (GenBank): AGY14277.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PB2 (373-381)
Chemical type: Peptide from protein
Sequence: ILRKATKRL
Position: 373-381
Source molecule (GenBank): AJJ96381.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PB2 (477-485)
Chemical type: Peptide from protein
Sequence: GVRVSKMGV
Position: 477-485
Source molecule (GenBank): AGY14277.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PB2 (503-511)
Chemical type: Peptide from protein
Sequence: RVRDQRGNV
Position: 503-511
Source molecule (GenBank): AGY14277.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PB2 (510-518)
Chemical type: Peptide from protein
Sequence: NVLLSPEEV
Position: 510-518
Source molecule (GenBank): AGY14277.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PB2 (605-613)
Chemical type: Peptide from protein
Sequence: DVLGTFDTV
Position: 605-613
Source molecule (GenBank): AGY14277.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: peptide 1
Chemical type: Peptide, no natural source
Sequence: MPYSSGHVV
Assay category: MhcBinding
Result: Positive

Epitope: peptide 2
Chemical type: Peptide, no natural source
Sequence: HALQTLDHV
Assay category: MhcBinding
Result: Positive

Epitope: peptide 3
Chemical type: Peptide, no natural source
Sequence: VVHKFVSKV
Assay category: MhcBinding
Result: Positive

Epitope: peptide 4
Chemical type: Peptide, no natural source
Sequence: SPMYFGHSV
Assay category: MhcBinding
Result: Positive

Epitope: peptide 5
Chemical type: Peptide, no natural source
Sequence: YAFVAHKVN
Assay category: MhcBinding
Result: Positive

Epitope: peptide 6
Chemical type: Peptide, no natural source
Sequence: MVYLTGHSV
Assay category: MhcBinding
Result: Positive

Epitope: peptide 11179
Chemical type: Peptide, no natural source
Sequence: SGHMMKAYL
Assay category: MhcBinding
Result: Positive-Low

Epitope: peptide 11180
Chemical type: Peptide, no natural source
Sequence: QGRLHPLLV
Assay category: MhcBinding
Result: Positive-Low

Epitope: H9N2 AIV peptide
Chemical type: Peptide from protein
Sequence: SGYSGIFSV
Position: 404-412
Source molecule (GenBank): ABV47445.1
Assay category: MhcBinding
Result: Positive-High

Epitope: H9N2 AIV peptide
Chemical type: Peptide from protein
Sequence: IAANIIGIL
Position: 28-36
Source molecule (GenBank): AJJ92227.1
Assay category: MhcBinding
Result: Positive-High

Epitope: H9N2 AIV peptide
Chemical type: Peptide from protein
Sequence: VIFNRLEAL
Position: 136-144
Source molecule (GenBank): AGY14287.1
Assay category: MhcBinding
Result: Positive-Intermediate

Epitope: H9N2 AIV peptide
Chemical type: Peptide from protein
Sequence: VAYERMCNI
Position: 217-225
Source molecule (GenBank): AGY14271.1
Assay category: MhcBinding
Result: Positive-Intermediate

Epitope: H9N2 AIV peptide
Chemical type: Peptide from protein
Sequence: GRRTRVAY
Position: 212-219
Source molecule (GenBank): AGY14271.1
Assay category: MhcBinding
Result: Negative

Epitope: H9N2 AIV peptide
Chemical type: Peptide from protein
Sequence: FQNWGIEPI
Position: 446-454
Source molecule (GenBank): AGY14277.1
Assay category: MhcBinding
Result: Negative

Epitope: ALV-J peptide
Chemical type: Peptide from protein
Sequence: GSLERPLLL
Position: 666-674
Source molecule (GenBank): AED98688.1
Assay category: MhcBinding
Result: Positive-High

Epitope: ALV-J peptide
Chemical type: Peptide from protein
Sequence: KAFTAWLEV
Position: 356-364
Source molecule (GenBank): AED98689.1
Assay category: MhcBinding
Result: Positive-High

Epitope: ALV-J peptide
Chemical type: Peptide from protein
Sequence: RALSKACNI
Position: 589-597
Source molecule (GenBank): AED98689.1
Assay category: MhcBinding
Result: Positive-Intermediate

Epitope: M2 (34-42)
Chemical type: Peptide from protein
Sequence: GILHLILWI
Position: 34-42
Source molecule (GenBank): ALA49103.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M2 (20-28)
Chemical type: Peptide from protein
Sequence: SGSSDPLVI
Position: 20-28
Source molecule (GenBank): ALA49103.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PB1 (204-212)
Chemical type: Peptide from protein
Sequence: TIGKKKQRL
Position: 204-212
Source molecule (GenBank): AJJ92415.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PB1 (711-719)
Chemical type: Peptide from protein
Sequence: ISSMVEAMV
Position: 711-719
Source molecule (GenBank): AJJ92415.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS1 (98-106)
Chemical type: Peptide from protein
Sequence: MSRDWLMLI
Position: 98-106
Source molecule (GenBank): AGY14287.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PB1 (80-88)
Chemical type: Peptide from protein
Sequence: SGYAQTDCV
Position: 80-88
Source molecule (GenBank): AJJ92415.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PB1 (244-252)
Chemical type: Peptide from protein
Sequence: PGMQIRGFV
Position: 244-252
Source molecule (GenBank): AJJ92415.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo