Reference: Laurent Gilardin; Sandrine Delignat; Ivan Peyron; Mathieu Ing; Yu-Chun Lone; Bagirath Gangadharan; Baptiste Michard; Yousra Kherabi; Meenu Sharma; Anastas Pashov; Jean-Baptiste Latouche; Mohamad Hamieh; Olivier Toutirais; Pascale Loiseau; Lionel Galicier; Agnès Veyradier; Srini Kaveri; Bernard Maillère; Paul Coppo; Sébastien Lacroix-Desmazes. "The ADAMTS13<sup>1239-1253</sup> peptide is a dominant HLA-DR1-restricted CD4<sup>+</sup> T-cell epitope." Haematologica (2017).

Epitope: ADAMTS13 589-603
Chemical type: Peptide from protein
Sequence: RPLFTHLAVRIGGRY
Position: 589-603
Source molecule (GenBank): AAL11095.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: ADAMTS13 589-603
Chemical type: Peptide from protein
Sequence: RPLFTHLAVRIGGRY
Position: 589-603
Source molecule (GenBank): AAL11095.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 0.46

Epitope: ADAMTS13 267-281
Chemical type: Peptide from protein
Sequence: RRQLLSLLSAGRARC
Position: 267-281
Source molecule (GenBank): AAL11095.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: ADAMTS13 267-281
Chemical type: Peptide from protein
Sequence: RRQLLSLLSAGRARC
Position: 267-281
Source molecule (GenBank): AAL11095.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 0.91

Epitope: ADAMTS13 371-385
Chemical type: Peptide from protein
Sequence: CRSLVELTPIAAVHG
Position: 371-385
Source molecule (GenBank): AAL11095.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: ADAMTS13 371-385
Chemical type: Peptide from protein
Sequence: CRSLVELTPIAAVHG
Position: 371-385
Source molecule (GenBank): AAL11095.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.59

Epitope: ADAMTS13 1239-1253
Chemical type: Peptide from protein
Sequence: GDMLLLWGRLTWRKM
Position: 1239-1253
Source molecule (GenBank): AAL11095.1
Assay category: TCell
Result: Positive-High
Host: M 25
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: ADAMTS13 1239-1253
Chemical type: Peptide from protein
Sequence: GDMLLLWGRLTWRKM
Position: 1239-1253
Source molecule (GenBank): AAL11095.1
Assay category: TCell
Result: Positive
Host: F 19
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: ADAMTS13 1239-1253
Chemical type: Peptide from protein
Sequence: GDMLLLWGRLTWRKM
Position: 1239-1253
Source molecule (GenBank): AAL11095.1
Assay category: TCell
Result: Positive
Host: F 34
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: ADAMTS13 1239-1253
Chemical type: Peptide from protein
Sequence: GDMLLLWGRLTWRKM
Position: 1239-1253
Source molecule (GenBank): AAL11095.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: ADAMTS13 1239-1253
Chemical type: Peptide from protein
Sequence: GDMLLLWGRLTWRKM
Position: 1239-1253
Source molecule (GenBank): AAL11095.1
Assay category: TCell
Result: Positive
Host: M 43
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: ADAMTS13 1239-1253
Chemical type: Peptide from protein
Sequence: GDMLLLWGRLTWRKM
Position: 1239-1253
Source molecule (GenBank): AAL11095.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.61

Epitope: ADAMTS13 1239-1253
Chemical type: Peptide from protein
Sequence: GDMLLLWGRLTWRKM
Position: 1239-1253
Source molecule (GenBank): AAL11095.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 0.083

Epitope: ADAMTS13 1239-1253
Chemical type: Peptide from protein
Sequence: GDMLLLWGRLTWRKM
Position: 1239-1253
Source molecule (GenBank): AAL11095.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 0.42

Epitope: ADAMTS13 1392-1406
Chemical type: Peptide from protein
Sequence: EGFLKAQASLRGQYW
Position: 1392-1406
Source molecule (GenBank): AAL11095.1
Assay category: TCell
Result: Positive
Host: M 25
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: ADAMTS13 1392-1406
Chemical type: Peptide from protein
Sequence: EGFLKAQASLRGQYW
Position: 1392-1406
Source molecule (GenBank): AAL11095.1
Assay category: TCell
Result: Positive
Host: M 43
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: ADAMTS13 1392-1406
Chemical type: Peptide from protein
Sequence: EGFLKAQASLRGQYW
Position: 1392-1406
Source molecule (GenBank): AAL11095.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: ADAMTS13 1392-1406
Chemical type: Peptide from protein
Sequence: EGFLKAQASLRGQYW
Position: 1392-1406
Source molecule (GenBank): AAL11095.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 3.74

Epitope: ADAMTS13 1355-1369
Chemical type: Peptide from protein
Sequence: ASYILIRDTHSLRTT
Position: 1355-1369
Source molecule (GenBank): AAL11095.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: ADAMTS13 1355-1369
Chemical type: Peptide from protein
Sequence: ASYILIRDTHSLRTT
Position: 1355-1369
Source molecule (GenBank): AAL11095.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 5.65

Epitope: ADAMTS13 774-788
Chemical type: Peptide from protein
Sequence: GSLLKTLPPARCRAG
Position: 774-788
Source molecule (GenBank): AAL11095.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: ADAMTS13 774-788
Chemical type: Peptide from protein
Sequence: GSLLKTLPPARCRAG
Position: 774-788
Source molecule (GenBank): AAL11095.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 7.11

Epitope: ADAMTS13 570-584
Chemical type: Peptide from protein
Sequence: YVTFLTVTPNLTSVY
Position: 570-584
Source molecule (GenBank): AAL11095.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: ADAMTS13 570-584
Chemical type: Peptide from protein
Sequence: YVTFLTVTPNLTSVY
Position: 570-584
Source molecule (GenBank): AAL11095.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 13.1

Epitope: ADAMTS13 914-928
Chemical type: Peptide from protein
Sequence: ELRFLCMDSALRVPV
Position: 914-928
Source molecule (GenBank): AAL11095.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: ADAMTS13 914-928
Chemical type: Peptide from protein
Sequence: ELRFLCMDSALRVPV
Position: 914-928
Source molecule (GenBank): AAL11095.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 12.6

Epitope: ADAMTS13 1224-1238
Chemical type: Peptide from protein
Sequence: VVTLRVLESSLNCSA
Position: 1224-1238
Source molecule (GenBank): AAL11095.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: ADAMTS13 1224-1238
Chemical type: Peptide from protein
Sequence: VVTLRVLESSLNCSA
Position: 1224-1238
Source molecule (GenBank): AAL11095.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 13.68

Epitope: ADAMTS13 1281-1295
Chemical type: Peptide from protein
Sequence: GVLLRYGSQLAPETF
Position: 1281-1295
Source molecule (GenBank): AAL11095.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: ADAMTS13 1281-1295
Chemical type: Peptide from protein
Sequence: GVLLRYGSQLAPETF
Position: 1281-1295
Source molecule (GenBank): AAL11095.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 28.34

Epitope: ADAMTS13
Chemical type: Peptide from protein
Sequence: GASFYHLGAAVPHSQ
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: ADAMTS13
Chemical type: Peptide from protein
Sequence: GASFYHLGAAVPHSQ
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 31.07

Epitope: ADAMTS13 140-154
Chemical type: Peptide from protein
Sequence: APNITANLTSSLLSV
Position: 140-154
Source molecule (GenBank): AAL11095.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 279.67

Epitope: ADAMTS13 308-322
Chemical type: Peptide from protein
Sequence: NEQCRVAFGPKAVAC
Position: 308-322
Source molecule (GenBank): AAL11095.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 5000.0

Epitope: ADAMTS13 111-125
Chemical type: Peptide from protein
Sequence: GAELLRDPSLGAQFR
Position: 111-125
Source molecule (GenBank): AAL11095.1
Assay category: TCell
Result: Negative
Host:  8-12 weeks
Cell type: lymph node cells
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: ADAMTS13 111-125
Chemical type: Peptide from protein
Sequence: GAELLRDPSLGAQFR
Position: 111-125
Source molecule (GenBank): AAL11095.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 10000.0

Epitope: ADAMTS13 1241-1251
Chemical type: Peptide from protein
Sequence: MLLLWGRLTWR
Position: 1241-1251
Source molecule (GenBank): AAL11095.1
Assay category: TCell
Result: Positive
Host:  8-12 weeks
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Cell Line / Clone