Reference: Richard B Kennedy; Gregory A Poland. "The identification of HLA class II-restricted T cell epitopes to vaccinia virus membrane proteins." Virology (2010).

Epitope: VACV-ACAM2000 peptide 1
Chemical type: Peptide from protein
Sequence: KADEDDNEETLKQRLT
Position: 37-52
Source molecule (GenBank): P20535.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 2
Chemical type: Peptide from protein
Sequence: VYSTCTVPTMNNAKLT
Position: 17-32
Source molecule (GenBank): P21115.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 3
Chemical type: Peptide from protein
Sequence: CTVPTMNNAKLTSTET
Position: 21-36
Source molecule (GenBank): P21115.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 4
Chemical type: Peptide from protein
Sequence: LYNKPLYEVNSTMTLS
Position: 85-100
Source molecule (GenBank): P21115.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 5
Chemical type: Peptide from protein
Sequence: PNAVCETDKWKYENPC
Position: 57-72
Source molecule (GenBank): P21115.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 8
Chemical type: Peptide from protein
Sequence: CETDKWKYENPCKKMC
Position: 61-76
Source molecule (GenBank): P21115.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 9
Chemical type: Peptide from protein
Sequence: TVYGDKIQGKNKRKRV
Position: 17-32
Source molecule (GenBank): P68616.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 10
Chemical type: Peptide from protein
Sequence: KITNVTTKFEQIEKCC
Position: 57-72
Source molecule (GenBank): P20535.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 11
Chemical type: Peptide from protein
Sequence: AFLIVRLNQCMSANEA
Position: 53-68
Source molecule (GenBank): P68616.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 12
Chemical type: Peptide from protein
Sequence: SSTTQYDHKESCNGLY
Position: 89-104
Source molecule (GenBank): P68616.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 13
Chemical type: Peptide from protein
Sequence: SGSTFSIGGVIHLSCK
Position: 197-212
Source molecule (GenBank): P21115.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 14
Chemical type: Peptide from protein
Sequence: CNLTVKNMCSADADAQ
Position: 49-64
Source molecule (GenBank): P20540.3
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 15
Chemical type: Peptide from protein
Sequence: NCAIKALMQLTTKATT
Position: 157-172
Source molecule (GenBank): P20540.3
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 16
Chemical type: Peptide from protein
Sequence: KCDIEIGNFYIRQNHG
Position: 33-48
Source molecule (GenBank): P20540.3
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 17
Chemical type: Peptide from protein
Sequence: QNVIIDECYGAPGSPT
Position: 129-144
Source molecule (GenBank): P20540.3
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 18
Chemical type: Peptide from protein
Sequence: GVIFLISVIVLVCSCD
Position: 289-304
Source molecule (GenBank): P21115.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 19
Chemical type: Peptide from protein
Sequence: AALFMYYAKRMLFTST
Position: 197-212
Source molecule (GenBank): P20540.3
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 20
Chemical type: Peptide from protein
Sequence: IRISMVISLLSMITMS
Position: 37-52
Source molecule (GenBank): P68616.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 22
Chemical type: Peptide from protein
Sequence: EQTSVFSATVYGDKIQ
Position: 9-24
Source molecule (GenBank): P68616.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 25
Chemical type: Peptide from protein
Sequence: KLEQEANASAQTKCDI
Position: 21-36
Source molecule (GenBank): P20540.3
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 26
Chemical type: Peptide from protein
Sequence: ITINCDVGYEVIGASY
Position: 153-168
Source molecule (GenBank): ACA50710.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 27
Chemical type: Peptide from protein
Sequence: IIVALTIMGVIFLISV
Position: 281-296
Source molecule (GenBank): P21115.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 28
Chemical type: Peptide from protein
Sequence: KATTQIAPRQVAGTGV
Position: 169-184
Source molecule (GenBank): P20540.3
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 29
Chemical type: Peptide from protein
Sequence: MYYAKRMLFTSTNDKI
Position: 201-216
Source molecule (GenBank): P20540.3
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 30
Chemical type: Peptide from protein
Sequence: DTFFRTSPMVIATTDM
Position: 233-248
Source molecule (GenBank): P20540.3
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 31
Chemical type: Peptide from protein
Sequence: QIAPRQVAGTGVQFYM
Position: 173-188
Source molecule (GenBank): P20540.3
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 32
Chemical type: Peptide from protein
Sequence: KRMLFTSTNDKIKLIL
Position: 205-220
Source molecule (GenBank): P20540.3
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 33
Chemical type: Peptide from protein
Sequence: ANKENVHWTTYMDTFF
Position: 221-236
Source molecule (GenBank): P20540.3
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 34
Chemical type: Peptide from protein
Sequence: VIILAALFMYYAKRML
Position: 193-208
Source molecule (GenBank): P20540.3
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 35
Chemical type: Peptide from protein
Sequence: NVHWTTYMDTFFRTSP
Position: 225-240
Source molecule (GenBank): P20540.3
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VACV-ACAM2000 peptide 36
Chemical type: Peptide from protein
Sequence: TTYMDTFFRTSPMVIA
Position: 229-244
Source molecule (GenBank): P20540.3
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VARV peptide 1
Chemical type: Peptide from protein
Sequence: KADGDDNEETLKQRLT
Position: 37-52
Source molecule (GenBank): ABF27326.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VARV peptide 4
Chemical type: Peptide from protein
Sequence: LYNKPLYEVNAIITLI
Position: 85-100
Source molecule (GenBank): ABF23141.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VARV peptide 6
Chemical type: Peptide from protein
Sequence: CYIFHSDYQLFSDAKA
Position: 108-123
Source molecule (GenBank): NP_042184.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VARV peptide 18
Chemical type: Peptide from protein
Sequence: GVIFLISVIVLVCSCN
Position: 289-304
Source molecule (GenBank): ABF23141.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VARV peptide 21
Chemical type: Peptide from protein
Sequence: DVLFRLENHAETLRAA
Position: 77-92
Source molecule (GenBank): ABF27326.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: VARV peptide 30
Chemical type: Peptide from protein
Sequence: DTFFRTSPMVIATTDI
Position: 233-248
Source molecule (GenBank): CAA53878.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo