Reference: Wei Jiang; James R Birtley; Shu-Chen Hung; Weiqi Wang; Shin-Heng Chiou; Claudia Macaubas; Birgitte Kornum; Lu Tian; Huang Huang; Lital Adler; Grant Weaver; Liying Lu; Alexandra Ilstad-Minnihan; Sriram Somasundaram; Sashi Ayyangar; Mark M Davis; Lawrence J Stern; Elizabeth D Mellins. "In vivo clonal expansion and phenotypes of hypocretin-specific CD4<sup>+</sup> T cells in narcolepsy patients and controls." Nature communications (2019).

Epitope: Hcrt 1-15
Chemical type: Peptide from protein
Sequence: MNLPSTKVSWAAVTL
Position: 1-15
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Positive-High

Epitope: Hcrt 5-19
Chemical type: Peptide from protein
Sequence: STKVSWAAVTLLLLL
Position: 5-19
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Negative

Epitope: Hcrt 9-23
Chemical type: Peptide from protein
Sequence: SWAAVTLLLLLLLLP
Position: 9-23
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Negative

Epitope: Hcrt 13-27
Chemical type: Peptide from protein
Sequence: VTLLLLLLLLPPALL
Position: 13-27
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Negative

Epitope: Hcrt 17-31
Chemical type: Peptide from protein
Sequence: LLLLLLPPALLSSGA
Position: 17-31
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Negative

Epitope: Hcrt 21-35
Chemical type: Peptide from protein
Sequence: LLPPALLSSGAAAQP
Position: 21-35
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Positive-Intermediate

Epitope: Hcrt 25-39
Chemical type: Peptide from protein
Sequence: ALLSSGAAAQPLPDC
Position: 25-39
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Positive-High

Epitope: Hcrt 29-43
Chemical type: Peptide from protein
Sequence: SGAAAQPLPDCCRQK
Position: 29-43
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Negative

Epitope: Hcrt 33-47
Chemical type: Peptide from protein
Sequence: AQPLPDCCRQKTCSC
Position: 33-47
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Negative

Epitope: Hcrt 37-51
Chemical type: Peptide from protein
Sequence: PDCCRQKTCSCRLYE
Position: 37-51
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Negative

Epitope: Hcrt 41-55
Chemical type: Peptide from protein
Sequence: RQKTCSCRLYELLHG
Position: 41-55
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Negative

Epitope: Hcrt 45-59
Chemical type: Peptide from protein
Sequence: CSCRLYELLHGAGNH
Position: 45-59
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Negative

Epitope: Hcrt 49-63
Chemical type: Peptide from protein
Sequence: LYELLHGAGNHAAGI
Position: 49-63
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Positive-Intermediate

Epitope: Hcrt 53-67
Chemical type: Peptide from protein
Sequence: LHGAGNHAAGILTLG
Position: 53-67
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Positive-High

Epitope: Hcrt 57-71
Chemical type: Peptide from protein
Sequence: GNHAAGILTLGKRRS
Position: 57-71
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Positive-Intermediate

Epitope: Hcrt 61-75
Chemical type: Peptide from protein
Sequence: AGILTLGKRRSGPPG
Position: 61-75
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Negative

Epitope: Hcrt 65-79
Chemical type: Peptide from protein
Sequence: TLGKRRSGPPGLQGR
Position: 65-79
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Negative

Epitope: Hcrt 69-83
Chemical type: Peptide from protein
Sequence: RRSGPPGLQGRLQRL
Position: 69-83
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Negative

Epitope: Hcrt 73-87
Chemical type: Peptide from protein
Sequence: PPGLQGRLQRLLQAS
Position: 73-87
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Negative

Epitope: Hcrt 77-91
Chemical type: Peptide from protein
Sequence: QGRLQRLLQASGNHA
Position: 77-91
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Negative

Epitope: Hcrt 81-95
Chemical type: Peptide from protein
Sequence: QRLLQASGNHAAGIL
Position: 81-95
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Positive-Intermediate

Epitope: Hcrt 85-99
Chemical type: Peptide from protein
Sequence: QASGNHAAGILTMGR
Position: 85-99
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Positive-High

Epitope: Hcrt 89-103
Chemical type: Peptide from protein
Sequence: NHAAGILTMGRRAGA
Position: 89-103
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Negative

Epitope: Hcrt 93-107
Chemical type: Peptide from protein
Sequence: GILTMGRRAGAEPAP
Position: 93-107
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Negative

Epitope: Hcrt 97-111
Chemical type: Peptide from protein
Sequence: MGRRAGAEPAPRPCL
Position: 97-111
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Negative

Epitope: Hcrt 101-115
Chemical type: Peptide from protein
Sequence: AGAEPAPRPCLGRRC
Position: 101-115
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Negative

Epitope: Hcrt 105-119
Chemical type: Peptide from protein
Sequence: PAPRPCLGRRCSAPA
Position: 105-119
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Negative

Epitope: Hcrt 109-123
Chemical type: Peptide from protein
Sequence: PCLGRRCSAPAAASV
Position: 109-123
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Negative

Epitope: Hcrt 113-127
Chemical type: Peptide from protein
Sequence: RRCSAPAAASVAPGG
Position: 113-127
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Positive-Intermediate

Epitope: Hcrt 117-131
Chemical type: Peptide from protein
Sequence: APAAASVAPGGQSGI
Position: 117-131
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Negative

Epitope: Hcrt 1-13
Chemical type: Peptide from protein
Sequence: MNLPSTKVSWAAV
Position: 1-13
Source molecule (GenBank): O43612.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: Blood
Culture conditions: Short Term Restimulated

Epitope: Hcrt 1-13
Chemical type: Peptide from protein
Sequence: MNLPSTKVSWAAV
Position: 1-13
Source molecule (GenBank): O43612.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: Blood
Culture conditions: Short Term Restimulated

Epitope: Hcrt 1-13
Chemical type: Peptide from protein
Sequence: MNLPSTKVSWAAV
Position: 1-13
Source molecule (GenBank): O43612.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: Blood
Culture conditions: Short Term Restimulated

Epitope: Hcrt 1-13
Chemical type: Peptide from protein
Sequence: MNLPSTKVSWAAV
Position: 1-13
Source molecule (GenBank): O43612.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: Blood
Culture conditions: Direct Ex Vivo

Epitope: Hcrt 1-13
Chemical type: Peptide from protein
Sequence: MNLPSTKVSWAAV
Position: 1-13
Source molecule (GenBank): O43612.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: Blood
Culture conditions: Short Term Restimulated

Epitope: Hcrt 56-69
Chemical type: Peptide from protein
Sequence: AGNHAAGILTLGKR
Position: 56-69
Source molecule (GenBank): O43612.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: Blood
Culture conditions: Short Term Restimulated

Epitope: Hcrt 56-69
Chemical type: Peptide from protein
Sequence: AGNHAAGILTLGKR
Position: 56-69
Source molecule (GenBank): O43612.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: Blood
Culture conditions: Short Term Restimulated

Epitope: Hcrt 56-69
Chemical type: Peptide from protein
Sequence: AGNHAAGILTLGKR
Position: 56-69
Source molecule (GenBank): O43612.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: Blood
Culture conditions: Direct Ex Vivo

Epitope: Hcrt 56-69
Chemical type: Peptide from protein
Sequence: AGNHAAGILTLGKR
Position: 56-69
Source molecule (GenBank): O43612.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: Blood
Culture conditions: Short Term Restimulated

Epitope: Hcrt 56-69
Chemical type: Peptide from protein
Sequence: AGNHAAGILTLGKR
Position: 56-69
Source molecule (GenBank): O43612.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: Blood
Culture conditions: Short Term Restimulated

Epitope: Hcrt 56-69
Chemical type: Peptide from protein
Sequence: AGNHAAGILTLGKR
Position: 56-69
Source molecule (GenBank): O43612.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 2.0

Epitope: Hcrt 87-100
Chemical type: Peptide from protein
Sequence: SGNHAAGILTMGRR
Position: 87-100
Source molecule (GenBank): O43612.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: Blood
Culture conditions: Short Term Restimulated

Epitope: Hcrt 87-100
Chemical type: Peptide from protein
Sequence: SGNHAAGILTMGRR
Position: 87-100
Source molecule (GenBank): O43612.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: Blood
Culture conditions: Direct Ex Vivo

Epitope: Hcrt 87-100
Chemical type: Peptide from protein
Sequence: SGNHAAGILTMGRR
Position: 87-100
Source molecule (GenBank): O43612.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: Blood
Culture conditions: Short Term Restimulated

Epitope: Hcrt 87-100
Chemical type: Peptide from protein
Sequence: SGNHAAGILTMGRR
Position: 87-100
Source molecule (GenBank): O43612.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: Blood
Culture conditions: Short Term Restimulated

Epitope: Hcrt 87-100
Chemical type: Peptide from protein
Sequence: SGNHAAGILTMGRR
Position: 87-100
Source molecule (GenBank): O43612.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: Blood
Culture conditions: Short Term Restimulated

Epitope: Hcrt 87-100
Chemical type: Peptide from protein
Sequence: SGNHAAGILTMGRR
Position: 87-100
Source molecule (GenBank): O43612.1
Assay category: TCell
Result: Positive
Cell type: Jurkat-T cell
Tissue: Blood
Culture conditions: Engineered

Epitope: Hcrt 87-100
Chemical type: Peptide from protein
Sequence: SGNHAAGILTMGRR
Position: 87-100
Source molecule (GenBank): O43612.1
Assay category: TCell
Result: Positive
Cell type: Jurkat-T cell
Tissue: Blood
Culture conditions: Engineered

Epitope: HCRT 25-37
Chemical type: Peptide from protein
Sequence: ALLSSGAAAQPLP
Position: 25-37
Source molecule (GenBank): O43612.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: Blood
Culture conditions: Direct Ex Vivo

Epitope: HCRT 25-37
Chemical type: Peptide from protein
Sequence: ALLSSGAAAQPLP
Position: 25-37
Source molecule (GenBank): O43612.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: Blood
Culture conditions: Short Term Restimulated

Epitope: HCRT 25-37
Chemical type: Peptide from protein
Sequence: ALLSSGAAAQPLP
Position: 25-37
Source molecule (GenBank): O43612.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: Blood
Culture conditions: Short Term Restimulated

Epitope: EBV 487-499
Chemical type: Peptide from protein
Sequence: ALLARSHVERTTD
Position: 487-499
Source molecule (GenBank): Q777E1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: Blood
Culture conditions: Short Term Restimulated

Epitope: EBV 487-499
Chemical type: Peptide from protein
Sequence: ALLARSHVERTTD
Position: 487-499
Source molecule (GenBank): Q777E1
Assay category: MhcBinding
Result: Positive-High