Reference: Alexander Greenshields-Watson; Meriem Attaf; Bruce J MacLachlan; Thomas Whalley; Cristina Rius; Aaron Wall; Angharad Lloyd; Hywel Hughes; Kathryn E Strange; Georgina H Mason; Andrea J Schauenburg; Sarah L Hulin-Curtis; James Geary; Yuan Chen; Sarah N Lauder; Kathryn Smart; Dhanasekaran Vijaykrishna; Miguel L Grau; Mikhail Shugay; Robert Andrews; Garry Dolton; Pierre J Rizkallah; Awen M Gallimore; Andrew K Sewell; Andrew J Godkin; David K Cole. "CD4<sup>+</sup> T Cells Recognize Conserved Influenza A Epitopes through Shared Patterns of V-Gene Usage and Complementary Biochemical Features." Cell reports (2020).

Epitope: QAR (M1 208-223)
Chemical type: Peptide from protein
Sequence: QARQMVQAMRTIGTHP
Position: 208-223
Source molecule (GenBank): P05777.2
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: QAR (M1 208-223)
Chemical type: Peptide from protein
Sequence: QARQMVQAMRTIGTHP
Position: 208-223
Source molecule (GenBank): P05777.2
Assay category: TCell
Result: Positive
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: QAR (M1 208-223)
Chemical type: Peptide from protein
Sequence: QARQMVQAMRTIGTHP
Position: 208-223
Source molecule (GenBank): P05777.2
Assay category: TCell
Result: Positive
Host:  20-60 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: QAR (M1 208-223)
Chemical type: Peptide from protein
Sequence: QARQMVQAMRTIGTHP
Position: 208-223
Source molecule (GenBank): P05777.2
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 1.64

Epitope: SGP (M1 17-30)
Chemical type: Peptide from protein
Sequence: SGPLKAEIAQRLED
Position: 17-30
Source molecule (GenBank): P05777.2
Assay category: TCell
Result: Positive-Low
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: SGP (M1 17-30)
Chemical type: Peptide from protein
Sequence: SGPLKAEIAQRLED
Position: 17-30
Source molecule (GenBank): P05777.2
Assay category: TCell
Result: Positive
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: SGP (M1 17-30)
Chemical type: Peptide from protein
Sequence: SGPLKAEIAQRLED
Position: 17-30
Source molecule (GenBank): P05777.2
Assay category: TCell
Result: Positive
Host:  20-60 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: SGP (M1 17-30)
Chemical type: Peptide from protein
Sequence: SGPLKAEIAQRLED
Position: 17-30
Source molecule (GenBank): P05777.2
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 2.66

Epitope: PB1 405-424
Chemical type: Peptide from protein
Sequence: PGMMMGMFNMLSTVLGVSIL
Position: 405-424
Source molecule (GenBank): P03431.1
Assay category: TCell
Result: Positive
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: PB1 405-424
Chemical type: Peptide from protein
Sequence: PGMMMGMFNMLSTVLGVSIL
Position: 405-424
Source molecule (GenBank): P03431.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 3.09

Epitope: HA 306-318
Chemical type: Peptide from protein
Sequence: PKYVKQNTLKLAT
Position: 306-318
Source molecule (GenBank): Q8V285.1
Assay category: TCell
Result: Positive
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HA 306-318
Chemical type: Peptide from protein
Sequence: PKYVKQNTLKLAT
Position: 306-318
Source molecule (GenBank): Q8V285.1
Assay category: TCell
Result: Positive
Quantitative measurement: 1.91

Epitope: HA 306-318
Chemical type: Peptide from protein
Sequence: PKYVKQNTLKLAT
Position: 306-318
Source molecule (GenBank): Q8V285.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 306-318
Chemical type: Peptide from protein
Sequence: PKYVKQNTLKLAT
Position: 306-318
Source molecule (GenBank): Q8V285.1
Assay category: TCell
Result: Positive
Host:  20-60 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HA 306-318
Chemical type: Peptide from protein
Sequence: PKYVKQNTLKLAT
Position: 306-318
Source molecule (GenBank): Q8V285.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 1.91

Epitope: GMF (PB1 410-422)
Chemical type: Peptide from protein
Sequence: GMFNMLSTVLGVS
Position: 410-422
Source molecule (GenBank): P03431.1
Assay category: TCell
Result: Positive-Low
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: GMF (PB1 410-422)
Chemical type: Peptide from protein
Sequence: GMFNMLSTVLGVS
Position: 410-422
Source molecule (GenBank): P03431.1
Assay category: TCell
Result: Positive
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GMF (PB1 410-422)
Chemical type: Peptide from protein
Sequence: GMFNMLSTVLGVS
Position: 410-422
Source molecule (GenBank): P03431.1
Assay category: TCell
Result: Positive
Host:  20-60 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: DPF (NP 302-314)
Chemical type: Peptide from protein
Sequence: DPFRLLQNSQVFS
Position: 302-314
Source molecule (GenBank): AAT70629.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: DPF (NP 302-314)
Chemical type: Peptide from protein
Sequence: DPFRLLQNSQVFS
Position: 302-314
Source molecule (GenBank): AAT70629.1
Assay category: TCell
Result: Positive
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: DPF (NP 302-314)
Chemical type: Peptide from protein
Sequence: DPFRLLQNSQVFS
Position: 302-314
Source molecule (GenBank): AAT70629.1
Assay category: TCell
Result: Positive
Host:  20-60 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: LPF (NP 417-430)
Chemical type: Peptide from protein
Sequence: LPFERATIMAAFT
Position: 418-430
Source molecule (GenBank): AAT70629.1
Assay category: TCell
Result: Negative
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: PTF (NP 409-421)
Chemical type: Peptide from protein
Sequence: PTFSVQRNLPFER
Position: 410-422
Source molecule (GenBank): AAT70629.1
Assay category: TCell
Result: Positive
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: NVV (PB-1 284-297)
Chemical type: Peptide from protein
Sequence: NVVRKMMTNSQDT
Position: 284-296
Source molecule (GenBank): P03431.1
Assay category: TCell
Result: Negative
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: IMF (PB-1 342-354)
Chemical type: Peptide from protein
Sequence: IMFSNKMARLGKG
Position: 342-354
Source molecule (GenBank): P03431.1
Assay category: TCell
Result: Negative
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: PB-1 peptide 29
Chemical type: Peptide from protein
Sequence: NVVRKMMTNSQDTELSFTIT
Position: 284-303
Source molecule (GenBank): P03431.2
Assay category: TCell
Result: Positive
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: PB-1 peptide 34
Chemical type: Peptide from protein
Sequence: NVLSIAPIMFSNKMARLGKG
Position: 335-354
Source molecule (GenBank): P03431.2
Assay category: TCell
Result: Positive
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: PB-1 peptide 38
Chemical type: Peptide from protein
Sequence: SIDLKYFNDSTRKKIEKIRP
Position: 375-394
Source molecule (GenBank): P03431.2
Assay category: TCell
Result: Negative
MHC allele types: 46
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: PB-1 peptide 43
Chemical type: Peptide from protein
Sequence: NLGQKRYTKTTYWWDGLQSS
Position: 425-444
Source molecule (GenBank): P03431.2
Assay category: TCell
Result: Negative
MHC allele types: 46
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: M1 peptide 2
Chemical type: Peptide from protein
Sequence: VLSIVPSGPLKAEIAQRLED
Position: 11-30
Source molecule (GenBank): P05777.2
Assay category: TCell
Result: Positive
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: M1 peptide 7
Chemical type: Peptide from protein
Sequence: GFVFTLTVPSERGLQRRRFV
Position: 61-80
Source molecule (GenBank): P05777.2
Assay category: TCell
Result: Negative
MHC allele types: 46
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: M1 peptide 13
Chemical type: Peptide from protein
Sequence: AGALASCMGLIYNRMGAVTT
Position: 121-140
Source molecule (GenBank): P05777.2
Assay category: TCell
Result: Positive
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: M1 peptide 14
Chemical type: Peptide from protein
Sequence: IYNRMGAVTTEVAFGLVCAT
Position: 131-150
Source molecule (GenBank): P05777.2
Assay category: TCell
Result: Positive
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: M1 peptide 22
Chemical type: Peptide from protein
Sequence: QMVQAMRTIGTHPSSSAGLK
Position: 211-230
Source molecule (GenBank): P05777.2
Assay category: TCell
Result: Positive
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: M1 peptide 23
Chemical type: Peptide from protein
Sequence: THPSSSAGLKDDLLENLQAY
Position: 221-240
Source molecule (GenBank): P05777.2
Assay category: TCell
Result: Negative
MHC allele types: 46
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: M1 peptide 24
Chemical type: Peptide from protein
Sequence: DDLLENLQAYQKRMGVQMQRFK
Position: 231-252
Source molecule (GenBank): P05777.2
Assay category: TCell
Result: Negative
MHC allele types: 46
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: NP peptide 24
Chemical type: Peptide from protein
Sequence: VAHKSCLPACVYGLAVASGY
Position: 270-289
Source molecule (GenBank): AAT70629.1
Assay category: TCell
Result: Negative
MHC allele types: 46
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: NP peptide 25
Chemical type: Peptide from protein
Sequence: VAHKSCLPACVYGLAVASGY
Position: 270-289
Source molecule (GenBank): AAT70629.1
Assay category: TCell
Result: Negative
MHC allele types: 46
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: NP peptide 26
Chemical type: Peptide from protein
Sequence: VYGLAVASGYDFEREGYSLV
Position: 280-299
Source molecule (GenBank): AAT70629.1
Assay category: TCell
Result: Negative
MHC allele types: 46
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: NP peptide 28
Chemical type: Peptide from protein
Sequence: GIDPFRLLQNSQVFSLIRPN
Position: 300-319
Source molecule (GenBank): AAT70629.1
Assay category: TCell
Result: Positive
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: NP peptide 38
Chemical type: Peptide from protein
Sequence: RASAGQISVQPTFSVQRNLPFER
Position: 400-422
Source molecule (GenBank): AAT70629.1
Assay category: TCell
Result: Positive
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: NP peptide 39
Chemical type: Peptide from protein
Sequence: QPTFSVQRNLPFERATIMAAFTG
Position: 409-431
Source molecule (GenBank): AAT70629.1
Assay category: TCell
Result: Positive
Host:  20-60 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated