Reference: Lin Zhang; Zhuolin Li; Ziche Tang; Lingxia Han; Xiaohui Wei; Xiaoli Xie; Shuaimeng Ren; Kai Meng; Yueyue Liu; Minli Xu; Lihong Qi; Hongyan Chen; Jiaqiang Wu; Nianzhi Zhang. "Efficient Identification of Tembusu Virus CTL Epitopes in Inbred HBW/B4 Ducks Using a Novel MHC Class I-Restricted Epitope Screening Scheme." Journal of immunology (Baltimore, Md. : 1950) (2022).

Epitope: TMUV NS3 1682-1690  (LRL9)
Chemical type: Peptide from protein
Sequence: LRKRQLTVL
Position: 1682-1690
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS3 1682-1690  (LRL9)
Chemical type: Peptide from protein
Sequence: LRKRQLTVL
Position: 1682-1690
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS3 1682-1690  (LRL9)
Chemical type: Peptide from protein
Sequence: LRKRQLTVL
Position: 1682-1690
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS3 1682-1690  (LRL9)
Chemical type: Peptide from protein
Sequence: LRKRQLTVL
Position: 1682-1690
Source molecule (GenBank): AEA72437.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 38.08

Epitope: TMUV NS3 1682-1690  (LRL9)
Chemical type: Peptide from protein
Sequence: LRKRQLTVL
Position: 1682-1690
Source molecule (GenBank): AEA72437.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 1.5

Epitope: VNNTQFVRF
Chemical type: Peptide, no natural source
Sequence: VNNTQFVRF
Assay category: MhcBinding
Result: Positive

Epitope: ERFYLERTY
Chemical type: Peptide, no natural source
Sequence: ERFYLERTY
Assay category: MhcBinding
Result: Positive

Epitope: MEFEPHSLH
Chemical type: Peptide, no natural source
Sequence: MEFEPHSLH
Assay category: MhcBinding
Result: Positive

Epitope: VRFGTTHLV
Chemical type: Peptide, no natural source
Sequence: VRFGTTHLV
Assay category: MhcBinding
Result: Positive

Epitope: TRFFYNPYR
Chemical type: Peptide, no natural source
Sequence: TRFFYNPYR
Assay category: MhcBinding
Result: Positive

Epitope: VDGHLLDHY
Chemical type: Peptide, no natural source
Sequence: VDGHLLDHY
Assay category: MhcBinding
Result: Positive

Epitope: LRFDYHYKR
Chemical type: Peptide, no natural source
Sequence: LRFDYHYKR
Assay category: MhcBinding
Result: Positive

Epitope: ERFFWVLSR
Chemical type: Peptide, no natural source
Sequence: ERFFWVLSR
Assay category: MhcBinding
Result: Positive

Epitope: LRRLHAFTA
Chemical type: Peptide, no natural source
Sequence: LRRLHAFTA
Assay category: MhcBinding
Result: Positive-Low

Epitope: EARPGQFTV
Chemical type: Peptide, no natural source
Sequence: EARPGQFTV
Assay category: MhcBinding
Result: Positive-Low

Epitope: TMUV C 7-15
Chemical type: Peptide from protein
Sequence: GRPGSGRVV
Position: 7-15
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TMUV C 7-15
Chemical type: Peptide from protein
Sequence: GRPGSGRVV
Position: 7-15
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TMUV C 7-15
Chemical type: Peptide from protein
Sequence: GRPGSGRVV
Position: 7-15
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TMUV C 7-15
Chemical type: Peptide from protein
Sequence: GRPGSGRVV
Position: 7-15
Source molecule (GenBank): AEA72437.1
Assay category: MhcBinding
Result: Positive

Epitope: TMUV C 23-31
Chemical type: Peptide from protein
Sequence: SRGNPLARI
Position: 23-31
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TMUV C 23-31
Chemical type: Peptide from protein
Sequence: SRGNPLARI
Position: 23-31
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TMUV C 23-31
Chemical type: Peptide from protein
Sequence: SRGNPLARI
Position: 23-31
Source molecule (GenBank): AEA72437.1
Assay category: MhcBinding
Result: Positive

Epitope: TMUV NS3 1684-1692
Chemical type: Peptide from protein
Sequence: KRQLTVLDL
Position: 1684-1692
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS3 1684-1692
Chemical type: Peptide from protein
Sequence: KRQLTVLDL
Position: 1684-1692
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS3 1684-1692
Chemical type: Peptide from protein
Sequence: KRQLTVLDL
Position: 1684-1692
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS3 1684-1692
Chemical type: Peptide from protein
Sequence: KRQLTVLDL
Position: 1684-1692
Source molecule (GenBank): AEA72437.1
Assay category: MhcBinding
Result: Positive

Epitope: TMUV NS3 1766-1774
Chemical type: Peptide from protein
Sequence: ARLLTPQRV
Position: 1766-1774
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS3 1766-1774
Chemical type: Peptide from protein
Sequence: ARLLTPQRV
Position: 1766-1774
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS3 1766-1774
Chemical type: Peptide from protein
Sequence: ARLLTPQRV
Position: 1766-1774
Source molecule (GenBank): AEA72437.1
Assay category: MhcBinding
Result: Positive

Epitope: TMUV NS3 1795-1803
Chemical type: Peptide from protein
Sequence: ARGYISTKV
Position: 1795-1803
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS3 1795-1803
Chemical type: Peptide from protein
Sequence: ARGYISTKV
Position: 1795-1803
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS3 1795-1803
Chemical type: Peptide from protein
Sequence: ARGYISTKV
Position: 1795-1803
Source molecule (GenBank): AEA72437.1
Assay category: MhcBinding
Result: Positive

Epitope: TMUV NS3 2028-2036
Chemical type: Peptide from protein
Sequence: QRKHFVEYL
Position: 2028-2036
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS3 2028-2036
Chemical type: Peptide from protein
Sequence: QRKHFVEYL
Position: 2028-2036
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS3 2028-2036
Chemical type: Peptide from protein
Sequence: QRKHFVEYL
Position: 2028-2036
Source molecule (GenBank): AEA72437.1
Assay category: MhcBinding
Result: Positive

Epitope: TMUV NS4A 2191-2199
Chemical type: Peptide from protein
Sequence: RRGLGKTGL
Position: 2191-2199
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS4A 2191-2199
Chemical type: Peptide from protein
Sequence: RRGLGKTGL
Position: 2191-2199
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS4A 2191-2199
Chemical type: Peptide from protein
Sequence: RRGLGKTGL
Position: 2191-2199
Source molecule (GenBank): AEA72437.1
Assay category: MhcBinding
Result: Positive

Epitope: TMUV NS4B 2242-2250
Chemical type: Peptide from protein
Sequence: QRSQTDSHL
Position: 2242-2250
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS4B 2242-2250
Chemical type: Peptide from protein
Sequence: QRSQTDSHL
Position: 2242-2250
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS4B 2242-2250
Chemical type: Peptide from protein
Sequence: QRSQTDSHL
Position: 2242-2250
Source molecule (GenBank): AEA72437.1
Assay category: MhcBinding
Result: Positive

Epitope: TMUV NS5 2993-3001
Chemical type: Peptide from protein
Sequence: SRAIWYMWL
Position: 2993-3001
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS5 2993-3001
Chemical type: Peptide from protein
Sequence: SRAIWYMWL
Position: 2993-3001
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS5 2993-3001
Chemical type: Peptide from protein
Sequence: SRAIWYMWL
Position: 2993-3001
Source molecule (GenBank): AEA72437.1
Assay category: MhcBinding
Result: Positive

Epitope: TMUV NS5 3083-3091
Chemical type: Peptide from protein
Sequence: HRAVAEPLI
Position: 3083-3091
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS5 3083-3091
Chemical type: Peptide from protein
Sequence: HRAVAEPLI
Position: 3083-3091
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: TMUV NS5 3083-3091
Chemical type: Peptide from protein
Sequence: HRAVAEPLI
Position: 3083-3091
Source molecule (GenBank): AEA72437.1
Assay category: MhcBinding
Result: Positive

Epitope: TMUV NS3 1682-1690  L1A
Chemical type: Peptide, no natural source
Sequence: ARKRQLTVL
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 40.47

Epitope: TMUV NS3 1682-1690 R2A
Chemical type: Peptide, no natural source
Sequence: LAKRQLTVL
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 21.99

Epitope: TMUV NS3 1682-1690 K3A
Chemical type: Peptide, no natural source
Sequence: LRARQLTVL
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 40.72

Epitope: TMUV NS3 1682-1690  R4A
Chemical type: Peptide, no natural source
Sequence: LRKAQLTVL
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 36.88

Epitope: TMUV NS3 1682-1690  Q5A
Chemical type: Peptide, no natural source
Sequence: LRKRALTVL
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 33.14

Epitope: TMUV NS3 1682-1690  L6A
Chemical type: Peptide, no natural source
Sequence: LRKRQATVL
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 35.1

Epitope: TMUV NS3 1682-1690 T7A
Chemical type: Peptide, no natural source
Sequence: LRKRQLAVL
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 37.41

Epitope: TMUV NS3 1682-1690  V8A
Chemical type: Peptide, no natural source
Sequence: LRKRQLTAL
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 38.51

Epitope: TMUV NS3 1682-1690  L9A
Chemical type: Peptide, no natural source
Sequence: LRKRQLTVA
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 44.95

Epitope: TMUV NS3 1682-1690  L9I
Chemical type: Peptide, no natural source
Sequence: LRKRQLTVI
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 41.27

Epitope: TMUV NS3 1682-1690  L9V
Chemical type: Peptide, no natural source
Sequence: LRKRQLTVV
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 47.18

Epitope: TMUV NS2B 1452-1460
Chemical type: Peptide from protein
Sequence: MRLLNDPGV
Position: 1452-1460
Source molecule (GenBank): AEA72437.1
Assay category: TCell
Result: Positive
Host:  7 days
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo