Reference: Leonard Moise; Ryan Tassone; Howard Latimer; Frances Terry; Lauren Levitz; John P Haran; Ted M Ross; Christine M Boyle; William D Martin; Anne S De Groot. "Immunization with cross-conserved H1N1 influenza CD4+ T-cell epitopes lowers viral burden in HLA DR3 transgenic mice." Human vaccines & immunotherapeutics (2013).

Epitope: HA 38-61
Chemical type: Peptide from protein
Sequence: DTVLEKNVTVHSVNLLENSH
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 38-61
Chemical type: Peptide from protein
Sequence: DTVLEKNVTVHSVNLLENSH
Assay category: TCell
Result: Negative
Host:  22-77 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HA 38-61
Chemical type: Peptide from protein
Sequence: DTVLEKNVTVHSVNLLENSH
Assay category: TCell
Result: Negative
Host:  22-77 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HA 38-61
Chemical type: Peptide from protein
Sequence: DTVLEKNVTVHSVNLLENSH
Assay category: TCell
Result: Positive
Host:  18-65 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HA 38-61
Chemical type: Peptide from protein
Sequence: DTVLEKNVTVHSVNLLENSH
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 30590.0

Epitope: HA 38-61
Chemical type: Peptide from protein
Sequence: DTVLEKNVTVHSVNLLENSH
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 990.0

Epitope: HA 38-61
Chemical type: Peptide from protein
Sequence: DTVLEKNVTVHSVNLLENSH
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 65320.0

Epitope: HA 38-61
Chemical type: Peptide from protein
Sequence: DTVLEKNVTVHSVNLLENSH
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 200000.0

Epitope: HA 38-61
Chemical type: Peptide from protein
Sequence: DTVLEKNVTVHSVNLLENSH
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 200000.0

Epitope: HA 38-61
Chemical type: Peptide from protein
Sequence: DTVLEKNVTVHSVNLLENSH
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 440.0

Epitope: HA 319-345
Chemical type: Peptide from protein
Sequence: CPRYVKQLTKLATGMRNVPE
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 319-345
Chemical type: Peptide from protein
Sequence: CPRYVKQLTKLATGMRNVPE
Assay category: TCell
Result: Positive
Host:  22-77 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HA 319-345
Chemical type: Peptide from protein
Sequence: CPRYVKQLTKLATGMRNVPE
Assay category: TCell
Result: Positive
Host:  22-77 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HA 319-345
Chemical type: Peptide from protein
Sequence: CPRYVKQLTKLATGMRNVPE
Assay category: TCell
Result: Positive-Low
Host:  18-65 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HA 319-345
Chemical type: Peptide from protein
Sequence: CPRYVKQLTKLATGMRNVPE
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 46710.0

Epitope: HA 319-345
Chemical type: Peptide from protein
Sequence: CPRYVKQLTKLATGMRNVPE
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1000.0

Epitope: HA 319-345
Chemical type: Peptide from protein
Sequence: CPRYVKQLTKLATGMRNVPE
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 79080.0

Epitope: HA 319-345
Chemical type: Peptide from protein
Sequence: CPRYVKQLTKLATGMRNVPE
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 10450.0

Epitope: HA 319-345
Chemical type: Peptide from protein
Sequence: CPRYVKQLTKLATGMRNVPE
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 1160.0

Epitope: HA 319-345
Chemical type: Peptide from protein
Sequence: CPRYVKQLTKLATGMRNVPE
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 29010.0

Epitope: HA 321-341
Chemical type: Peptide from protein
Sequence: CPKYVRSAKLRMVTGLRNIPS
Position: 315-335
Source molecule (GenBank): CAA91082.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 321-341
Chemical type: Peptide from protein
Sequence: CPKYVRSAKLRMVTGLRNIPS
Position: 315-335
Source molecule (GenBank): CAA91082.1
Assay category: TCell
Result: Positive
Host:  22-77 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HA 321-341
Chemical type: Peptide from protein
Sequence: CPKYVRSAKLRMVTGLRNIPS
Position: 315-335
Source molecule (GenBank): CAA91082.1
Assay category: TCell
Result: Positive
Host:  22-77 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HA 321-341
Chemical type: Peptide from protein
Sequence: CPKYVRSAKLRMVTGLRNIPS
Position: 315-335
Source molecule (GenBank): CAA91082.1
Assay category: TCell
Result: Negative
Host:  18-65 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HA 321-341
Chemical type: Peptide from protein
Sequence: CPKYVRSAKLRMVTGLRNIPS
Position: 315-335
Source molecule (GenBank): CAA91082.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 200000.0

Epitope: HA 321-341
Chemical type: Peptide from protein
Sequence: CPKYVRSAKLRMVTGLRNIPS
Position: 315-335
Source molecule (GenBank): CAA91082.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 200000.0

Epitope: HA 321-341
Chemical type: Peptide from protein
Sequence: CPKYVRSAKLRMVTGLRNIPS
Position: 315-335
Source molecule (GenBank): CAA91082.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 4130.0

Epitope: HA 321-341
Chemical type: Peptide from protein
Sequence: CPKYVRSAKLRMVTGLRNIPS
Position: 315-335
Source molecule (GenBank): CAA91082.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 53740.0

Epitope: HA 321-341
Chemical type: Peptide from protein
Sequence: CPKYVRSAKLRMVTGLRNIPS
Position: 315-335
Source molecule (GenBank): CAA91082.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 3240.0

Epitope: HA 321-341
Chemical type: Peptide from protein
Sequence: CPKYVRSAKLRMVTGLRNIPS
Position: 315-335
Source molecule (GenBank): CAA91082.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 680.0

Epitope: HA 396-417
Chemical type: Peptide from protein
Sequence: NSVIEKMNTQFTAVGKEFNKHE
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 396-417
Chemical type: Peptide from protein
Sequence: NSVIEKMNTQFTAVGKEFNKHE
Assay category: TCell
Result: Positive
Host:  22-77 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HA 396-417
Chemical type: Peptide from protein
Sequence: NSVIEKMNTQFTAVGKEFNKHE
Assay category: TCell
Result: Positive
Host:  22-77 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HA 396-417
Chemical type: Peptide from protein
Sequence: NSVIEKMNTQFTAVGKEFNKHE
Assay category: TCell
Result: Positive
Host:  18-65 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HA 396-417
Chemical type: Peptide from protein
Sequence: NSVIEKMNTQFTAVGKEFNKHE
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 5840.0

Epitope: HA 396-417
Chemical type: Peptide from protein
Sequence: NSVIEKMNTQFTAVGKEFNKHE
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1000.0

Epitope: HA 396-417
Chemical type: Peptide from protein
Sequence: NSVIEKMNTQFTAVGKEFNKHE
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 200000.0

Epitope: HA 396-417
Chemical type: Peptide from protein
Sequence: NSVIEKMNTQFTAVGKEFNKHE
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 2600.0

Epitope: HA 396-417
Chemical type: Peptide from protein
Sequence: NSVIEKMNTQFTAVGKEFNKHE
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 200000.0

Epitope: HA 396-417
Chemical type: Peptide from protein
Sequence: NSVIEKMNTQFTAVGKEFNKHE
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 590.0

Epitope: HA 432-453
Chemical type: Peptide from protein
Sequence: IDIWTYNAELLVLLENERTDFHDS
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 432-453
Chemical type: Peptide from protein
Sequence: IDIWTYNAELLVLLENERTDFHDS
Assay category: TCell
Result: Positive
Host:  22-77 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HA 432-453
Chemical type: Peptide from protein
Sequence: IDIWTYNAELLVLLENERTDFHDS
Assay category: TCell
Result: Positive
Host:  22-77 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HA 432-453
Chemical type: Peptide from protein
Sequence: IDIWTYNAELLVLLENERTDFHDS
Assay category: TCell
Result: Positive-Low
Host:  18-65 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HA 432-453
Chemical type: Peptide from protein
Sequence: IDIWTYNAELLVLLENERTDFHDS
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 200000.0

Epitope: HA 432-453
Chemical type: Peptide from protein
Sequence: IDIWTYNAELLVLLENERTDFHDS
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1000.0

Epitope: HA 432-453
Chemical type: Peptide from protein
Sequence: IDIWTYNAELLVLLENERTDFHDS
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 1090.0

Epitope: HA 432-453
Chemical type: Peptide from protein
Sequence: IDIWTYNAELLVLLENERTDFHDS
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 1650.0

Epitope: HA 432-453
Chemical type: Peptide from protein
Sequence: IDIWTYNAELLVLLENERTDFHDS
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 1690.0

Epitope: HA 432-453
Chemical type: Peptide from protein
Sequence: IDIWTYNAELLVLLENERTDFHDS
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 18840.0

Epitope: HA 456-480
Chemical type: Peptide from protein
Sequence: KNLYEKVKSQLKNNAKEEIGNGC
Assay category: TCell
Result: Positive-Low
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 456-480
Chemical type: Peptide from protein
Sequence: KNLYEKVKSQLKNNAKEEIGNGC
Assay category: TCell
Result: Positive
Host:  22-77 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HA 456-480
Chemical type: Peptide from protein
Sequence: KNLYEKVKSQLKNNAKEEIGNGC
Assay category: TCell
Result: Positive-Low
Host:  18-65 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HA 456-480
Chemical type: Peptide from protein
Sequence: KNLYEKVKSQLKNNAKEEIGNGC
Assay category: TCell
Result: Positive
Host:  22-77 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HA 456-480
Chemical type: Peptide from protein
Sequence: KNLYEKVKSQLKNNAKEEIGNGC
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 32660.0

Epitope: HA 456-480
Chemical type: Peptide from protein
Sequence: KNLYEKVKSQLKNNAKEEIGNGC
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 200000.0

Epitope: HA 456-480
Chemical type: Peptide from protein
Sequence: KNLYEKVKSQLKNNAKEEIGNGC
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 11980.0

Epitope: HA 456-480
Chemical type: Peptide from protein
Sequence: KNLYEKVKSQLKNNAKEEIGNGC
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 200000.0

Epitope: HA 456-480
Chemical type: Peptide from protein
Sequence: KNLYEKVKSQLKNNAKEEIGNGC
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 200000.0

Epitope: HA 456-480
Chemical type: Peptide from protein
Sequence: KNLYEKVKSQLKNNAKEEIGNGC
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 500.0

Epitope: HA 516-540
Chemical type: Peptide from protein
Sequence: IDGVKLESMGVYQILAIYSTVASSL
Position: 516-540
Source molecule (GenBank): AAA79714.1
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 516-540
Chemical type: Peptide from protein
Sequence: IDGVKLESMGVYQILAIYSTVASSL
Position: 516-540
Source molecule (GenBank): AAA79714.1
Assay category: TCell
Result: Positive
Host:  22-77 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HA 516-540
Chemical type: Peptide from protein
Sequence: IDGVKLESMGVYQILAIYSTVASSL
Position: 516-540
Source molecule (GenBank): AAA79714.1
Assay category: TCell
Result: Positive
Host:  22-77 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HA 516-540
Chemical type: Peptide from protein
Sequence: IDGVKLESMGVYQILAIYSTVASSL
Position: 516-540
Source molecule (GenBank): AAA79714.1
Assay category: TCell
Result: Positive
Host:  18-65 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HA 516-540
Chemical type: Peptide from protein
Sequence: IDGVKLESMGVYQILAIYSTVASSL
Position: 516-540
Source molecule (GenBank): AAA79714.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 10030.0

Epitope: HA 516-540
Chemical type: Peptide from protein
Sequence: IDGVKLESMGVYQILAIYSTVASSL
Position: 516-540
Source molecule (GenBank): AAA79714.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 200000.0

Epitope: HA 516-540
Chemical type: Peptide from protein
Sequence: IDGVKLESMGVYQILAIYSTVASSL
Position: 516-540
Source molecule (GenBank): AAA79714.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 1790.0

Epitope: HA 516-540
Chemical type: Peptide from protein
Sequence: IDGVKLESMGVYQILAIYSTVASSL
Position: 516-540
Source molecule (GenBank): AAA79714.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 81490.0

Epitope: HA 516-540
Chemical type: Peptide from protein
Sequence: IDGVKLESMGVYQILAIYSTVASSL
Position: 516-540
Source molecule (GenBank): AAA79714.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 9610.0

Epitope: HA 516-540
Chemical type: Peptide from protein
Sequence: IDGVKLESMGVYQILAIYSTVASSL
Position: 516-540
Source molecule (GenBank): AAA79714.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 110310.0

Epitope: HA 548-563
Chemical type: Peptide from protein
Sequence: AISFWFMCSNGSLQCRI
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 548-563
Chemical type: Peptide from protein
Sequence: AISFWFMCSNGSLQCRI
Assay category: TCell
Result: Positive
Host:  22-77 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HA 548-563
Chemical type: Peptide from protein
Sequence: AISFWFMCSNGSLQCRI
Assay category: TCell
Result: Positive
Host:  22-77 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HA 548-563
Chemical type: Peptide from protein
Sequence: AISFWFMCSNGSLQCRI
Assay category: TCell
Result: Negative
Host:  18-65 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HA 548-563
Chemical type: Peptide from protein
Sequence: AISFWFMCSNGSLQCRI
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 200000.0

Epitope: HA 548-563
Chemical type: Peptide from protein
Sequence: AISFWFMCSNGSLQCRI
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 10680.0

Epitope: HA 548-563
Chemical type: Peptide from protein
Sequence: AISFWFMCSNGSLQCRI
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 4520.0

Epitope: HA 548-563
Chemical type: Peptide from protein
Sequence: AISFWFMCSNGSLQCRI
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 62950.0

Epitope: HA 548-563
Chemical type: Peptide from protein
Sequence: AISFWFMCSNGSLQCRI
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 200000.0

Epitope: HA 548-563
Chemical type: Peptide from protein
Sequence: AISFWFMCSNGSLQCRI
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 200000.0

Epitope: NA 129-150
Chemical type: Peptide from protein
Sequence: CRTFFLTQGALLNDKHSNGTVK
Position: 129-150
Source molecule (GenBank): AAF77045.1
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NA 129-150
Chemical type: Peptide from protein
Sequence: CRTFFLTQGALLNDKHSNGTVK
Position: 129-150
Source molecule (GenBank): AAF77045.1
Assay category: TCell
Result: Positive
Host:  22-77 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: NA 129-150
Chemical type: Peptide from protein
Sequence: CRTFFLTQGALLNDKHSNGTVK
Position: 129-150
Source molecule (GenBank): AAF77045.1
Assay category: TCell
Result: Positive
Host:  22-77 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: NA 129-150
Chemical type: Peptide from protein
Sequence: CRTFFLTQGALLNDKHSNGTVK
Position: 129-150
Source molecule (GenBank): AAF77045.1
Assay category: TCell
Result: Negative
Host:  18-65 years
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NA 129-150
Chemical type: Peptide from protein
Sequence: CRTFFLTQGALLNDKHSNGTVK
Position: 129-150
Source molecule (GenBank): AAF77045.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 13060.0

Epitope: NA 129-150
Chemical type: Peptide from protein
Sequence: CRTFFLTQGALLNDKHSNGTVK
Position: 129-150
Source molecule (GenBank): AAF77045.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 4370.0

Epitope: NA 129-150
Chemical type: Peptide from protein
Sequence: CRTFFLTQGALLNDKHSNGTVK
Position: 129-150
Source molecule (GenBank): AAF77045.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1000.0

Epitope: NA 129-150
Chemical type: Peptide from protein
Sequence: CRTFFLTQGALLNDKHSNGTVK
Position: 129-150
Source molecule (GenBank): AAF77045.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1000.0

Epitope: NA 129-150
Chemical type: Peptide from protein
Sequence: CRTFFLTQGALLNDKHSNGTVK
Position: 129-150
Source molecule (GenBank): AAF77045.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 49710.0

Epitope: NA 129-150
Chemical type: Peptide from protein
Sequence: CRTFFLTQGALLNDKHSNGTVK
Position: 129-150
Source molecule (GenBank): AAF77045.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 29810.0