Reference: Elodie Picarda; Séverine Bézie; Lorena Usero; Jason Ossart; Marine Besnard; Hanim Halim; Klara Echasserieau; Claire Usal; Jamie Rossjohn; Karine Bernardeau; Stéphanie Gras; Carole Guillonneau. "Cross-Reactive Donor-Specific CD8<sup>+</sup> Tregs Efficiently Prevent Transplant Rejection." Cell reports (2019).

Epitope: Bu31-10 peptide
Chemical type: Peptide from protein
Sequence: YLRYDSDVGEYR
Position: 38-49
Source molecule (GenBank): C60497
Assay category: TCell
Result: Positive
Host: M 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Bu31-10 peptide
Chemical type: Peptide from protein
Sequence: YLRYDSDVGEYR
Position: 38-49
Source molecule (GenBank): C60497
Assay category: TCell
Result: Positive
Host: M 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Bu31-10 peptide
Chemical type: Peptide from protein
Sequence: YLRYDSDVGEYR
Position: 38-49
Source molecule (GenBank): C60497
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 62.4

Epitope: Bu31-10 peptide
Chemical type: Peptide from protein
Sequence: YLRYDSDVGEYR
Position: 38-49
Source molecule (GenBank): C60497
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 2.9

Epitope: Du51 peptide
Chemical type: Peptide from protein
Sequence: NREEYARFDSDVGEYR
Position: 60-75
Source molecule (GenBank): AAD39084.1
Assay category: TCell
Result: Positive
Host: M 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Du51 peptide
Chemical type: Peptide from protein
Sequence: NREEYARFDSDVGEYR
Position: 60-75
Source molecule (GenBank): AAD39084.1
Assay category: TCell
Result: Positive
Host: M 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Du51 peptide
Chemical type: Peptide from protein
Sequence: NREEYARFDSDVGEYR
Position: 60-75
Source molecule (GenBank): AAD39084.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 50.9

Epitope: Bu31-1 peptide
Chemical type: Peptide from protein
Sequence: YLRYDSDVG
Position: 38-46
Source molecule (GenBank): C60497
Assay category: TCell
Result: Negative
Host: M 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Bu31-2 peptide
Chemical type: Peptide from protein
Sequence: LRYDSDVGE
Position: 39-47
Source molecule (GenBank): C60497
Assay category: TCell
Result: Negative
Host: M 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Bu31-3 peptide
Chemical type: Peptide from protein
Sequence: RYDSDVGEY
Position: 40-48
Source molecule (GenBank): C60497
Assay category: TCell
Result: Negative
Host: M 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Bu31-4 peptide
Chemical type: Peptide from protein
Sequence: YDSDVGEYR
Position: 41-49
Source molecule (GenBank): C60497
Assay category: TCell
Result: Negative
Host: M 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Bu31-5 peptide
Chemical type: Peptide from protein
Sequence: DSDVGEYRA
Position: 42-50
Source molecule (GenBank): C60497
Assay category: TCell
Result: Negative
Host: M 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Bu31-6 peptide
Chemical type: Peptide from protein
Sequence: SDVGEYRAV
Position: 43-51
Source molecule (GenBank): C60497
Assay category: TCell
Result: Negative
Host: M 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Bu31-7 peptide
Chemical type: Peptide from protein
Sequence: DVGEYRAVT
Position: 44-52
Source molecule (GenBank): C60497
Assay category: TCell
Result: Negative
Host: M 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Bu31-8 peptide
Chemical type: Peptide from protein
Sequence: VGEYRAVTE
Position: 45-53
Source molecule (GenBank): C60497
Assay category: TCell
Result: Negative
Host: M 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Bu31-9 peptide
Chemical type: Peptide from protein
Sequence: GEYRAVTELGR
Position: 46-56
Source molecule (GenBank): C60497
Assay category: TCell
Result: Negative
Host: M 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Bu31-11 peptide
Chemical type: Peptide from protein
Sequence: VGEYRAVTELGR
Position: 45-56
Source molecule (GenBank): C60497
Assay category: TCell
Result: Negative
Host: M 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Bu31-12 peptide
Chemical type: Peptide from protein
Sequence: DVGEYRAVTELGR
Position: 44-56
Source molecule (GenBank): C60497
Assay category: TCell
Result: Negative
Host: M 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Bu31-13 peptide
Chemical type: Peptide from protein
Sequence: YLRYDSDVGEYRAV
Position: 38-51
Source molecule (GenBank): C60497
Assay category: TCell
Result: Positive
Host: M 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Bu31-14 peptide
Chemical type: Peptide from protein
Sequence: SDVGEYRAVTELGR
Position: 43-56
Source molecule (GenBank): C60497
Assay category: TCell
Result: Positive
Host: M 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Bu31-15 peptide
Chemical type: Peptide from protein
Sequence: LRYDSDVGEYRAVTE
Position: 39-53
Source molecule (GenBank): C60497
Assay category: TCell
Result: Positive
Host: M 8-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Hpep1
Chemical type: Peptide from protein
Sequence: NQEESVRFDSDVGEFR
Position: 62-77
Source molecule (GenBank): NP_002115.2
Assay category: TCell
Result: Positive
MHC allele types: 143
Host: M 
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Hpep2
Chemical type: Peptide from protein
Sequence: NREEYARFDSDVGEFR
Position: 60-75
Source molecule (GenBank): AAV38583.1
Assay category: TCell
Result: Positive
MHC allele types: 143
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Hpep2
Chemical type: Peptide from protein
Sequence: NREEYARFDSDVGEFR
Position: 60-75
Source molecule (GenBank): AAV38583.1
Assay category: TCell
Result: Positive
MHC allele types: 143
Host: M 
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Hpep3
Chemical type: Peptide from protein
Sequence: NREEYARFDSDVGVYR
Position: 65-80
Source molecule (GenBank): P01920.1
Assay category: TCell
Result: Negative
MHC allele types: 143
Host: M 
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Hpep4
Chemical type: Peptide from protein
Sequence: NREEYVRFDSDVGEYR
Position: 28-43
Source molecule (GenBank): AAR29079.1
Assay category: TCell
Result: Positive
MHC allele types: 143
Host: M 
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo