Reference: Tim D Jones; Arron R Hearn; Robert G E Holgate; Dorota Kozub; Mark H Fogg; Francis J Carr; Matthew P Baker; Javier Lacadena; Kurt R Gehlsen. "A deimmunised form of the ribotoxin, α-sarcin, lacking CD4+ T cell epitopes and its use as an immunotoxin warhead." Protein engineering, design & selection : PEDS (2016).

Epitope: α-sarcin 1-15
Chemical type: Peptide from protein
Sequence: AVTWTCLNDQKNPKT
Position: 28-42
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 4-18
Chemical type: Peptide from protein
Sequence: WTCLNDQKNPKTNKY
Position: 31-45
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 7-21
Chemical type: Peptide from protein
Sequence: LNDQKNPKTNKYETK
Position: 34-48
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 10-24
Chemical type: Peptide from protein
Sequence: QKNPKTNKYETKRLL
Position: 37-51
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 13-27
Chemical type: Peptide from protein
Sequence: PKTNKYETKRLLYNQ
Position: 40-54
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 16-30
Chemical type: Peptide from protein
Sequence: NKYETKRLLYNQNKA
Position: 43-57
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 19-33
Chemical type: Peptide from protein
Sequence: ETKRLLYNQNKAESN
Position: 46-60
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 25-39
Chemical type: Peptide from protein
Sequence: YNQNKAESNSHHAPL
Position: 52-66
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 28-42
Chemical type: Peptide from protein
Sequence: NKAESNSHHAPLSDG
Position: 55-69
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 31-45
Chemical type: Peptide from protein
Sequence: ESNSHHAPLSDGKTG
Position: 58-72
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 34-48
Chemical type: Peptide from protein
Sequence: SHHAPLSDGKTGSSY
Position: 61-75
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 37-51
Chemical type: Peptide from protein
Sequence: APLSDGKTGSSYPHW
Position: 64-78
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 40-54
Chemical type: Peptide from protein
Sequence: SDGKTGSSYPHWFTN
Position: 67-81
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 43-57
Chemical type: Peptide from protein
Sequence: KTGSSYPHWFTNGYD
Position: 70-84
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 46-60
Chemical type: Peptide from protein
Sequence: SSYPHWFTNGYDGDG
Position: 73-87
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 52-66
Chemical type: Peptide from protein
Sequence: FTNGYDGDGKLPKGR
Position: 79-93
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 55-69
Chemical type: Peptide from protein
Sequence: GYDGDGKLPKGRTPI
Position: 82-96
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 58-72
Chemical type: Peptide from protein
Sequence: GDGKLPKGRTPIKFG
Position: 85-99
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 64-78
Chemical type: Peptide from protein
Sequence: KGRTPIKFGKSDCDR
Position: 91-105
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 67-81
Chemical type: Peptide from protein
Sequence: TPIKFGKSDCDRPPK
Position: 94-108
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 70-84
Chemical type: Peptide from protein
Sequence: KFGKSDCDRPPKHSK
Position: 97-111
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 73-87
Chemical type: Peptide from protein
Sequence: KSDCDRPPKHSKDGN
Position: 100-114
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 76-90
Chemical type: Peptide from protein
Sequence: CDRPPKHSKDGNGKT
Position: 103-117
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 79-93
Chemical type: Peptide from protein
Sequence: PPKHSKDGNGKTDHY
Position: 106-120
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 88-102
Chemical type: Peptide from protein
Sequence: GKTDHYLLEFPTFPD
Position: 115-129
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 91-105
Chemical type: Peptide from protein
Sequence: DHYLLEFPTFPDGHD
Position: 118-132
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 97-111
Chemical type: Peptide from protein
Sequence: FPTFPDGHDYKFDSK
Position: 124-138
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 100-114
Chemical type: Peptide from protein
Sequence: FPDGHDYKFDSKKPK
Position: 127-141
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 103-117
Chemical type: Peptide from protein
Sequence: GHDYKFDSKKPKENP
Position: 130-144
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 109-123
Chemical type: Peptide from protein
Sequence: DSKKPKENPGPARVI
Position: 136-150
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 115-129
Chemical type: Peptide from protein
Sequence: ENPGPARVIYTYPNK
Position: 142-156
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 118-132
Chemical type: Peptide from protein
Sequence: GPARVIYTYPNKVFC
Position: 145-159
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 121-135
Chemical type: Peptide from protein
Sequence: RVIYTYPNKVFCGII
Position: 148-162
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 124-138
Chemical type: Peptide from protein
Sequence: YTYPNKVFCGIIAHT
Position: 151-165
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 127-141
Chemical type: Peptide from protein
Sequence: PNKVFCGIIAHTKEN
Position: 154-168
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 133-147
Chemical type: Peptide from protein
Sequence: GIIAHTKENQGELKL
Position: 160-174
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 136-150
Chemical type: Peptide from protein
Sequence: AHTKENQGELKLCSH
Position: 163-177
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: α-sarcin 10-18
Chemical type: Peptide from protein
Sequence: QKNPKTNKY
Position: 37-45
Source molecule (GenBank): BAA02863
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo