Reference: Yang Chen; Min Shen; Yong Gu; Xinyu Xu; Lingling Bian; Fan Yang; Shuang Chen; Li Ji; Jin Liu; Jing Zhu; Zheng Zhang; Qi Fu; Yun Cai; Heng Chen; Kuanfeng Xu; Min Sun; Xuqin Zheng; Jie Shen; Hongwen Zhou; Mei Zhang; Kathryn Haskins; Liping Yu; Tao Yang; Yun Shi. "Pivotal epitopes for islet antigen-specific CD8<sup>+</sup> T cell detection improve classification of suspected type 1 diabetes with the HLA-A*0201 allele." Immunologic research (2025).

Epitope: GAD65 536-545
Chemical type: Peptide from protein
Sequence: RMMEYGTTMV
Position: 536-545
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GAD65 536-545
Chemical type: Peptide from protein
Sequence: RMMEYGTTMV
Position: 536-545
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ZnT8 291-300
Chemical type: Peptide from protein
Sequence: ILAVDGVLSV
Position: 291-300
Source molecule (GenBank): Q8IWU4.2
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ZnT8 291-300
Chemical type: Peptide from protein
Sequence: ILAVDGVLSV
Position: 291-300
Source molecule (GenBank): Q8IWU4.2
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ZnT8 153-161
Chemical type: Peptide from protein
Sequence: VVTGVLVYL
Position: 153-161
Source molecule (GenBank): Q8IWU4.2
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ZnT8 153-161
Chemical type: Peptide from protein
Sequence: VVTGVLVYL
Position: 153-161
Source molecule (GenBank): Q8IWU4.2
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: IGRP 152-160
Chemical type: Peptide from protein
Sequence: FLWSVFWLI
Position: 152-160
Source molecule (GenBank): Q9NQR9.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: IGRP 152-160
Chemical type: Peptide from protein
Sequence: FLWSVFWLI
Position: 152-160
Source molecule (GenBank): Q9NQR9.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PI B18-27 (PPI 42-51)
Chemical type: Peptide from protein
Sequence: VCGERGFFYT
Position: 42-51
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PI B18-27 (PPI 42-51)
Chemical type: Peptide from protein
Sequence: VCGERGFFYT
Position: 42-51
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: IGRP 215-223
Chemical type: Peptide from protein
Sequence: FLFAVGFYL
Position: 215-223
Source molecule (GenBank): Q9NQR9.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: IGRP 215-223
Chemical type: Peptide from protein
Sequence: FLFAVGFYL
Position: 215-223
Source molecule (GenBank): Q9NQR9.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PI C29-A5 (PPI 85-94)
Chemical type: Peptide from protein
Sequence: SLQKRGIVEQ
Position: 85-94
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PI C29-A5 (PPI 85-94)
Chemical type: Peptide from protein
Sequence: SLQKRGIVEQ
Position: 85-94
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ZnT8 115-123
Chemical type: Peptide from protein
Sequence: LLSLFSLWL
Position: 115-123
Source molecule (GenBank): Q8IWU4.2
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ZnT8 115-123
Chemical type: Peptide from protein
Sequence: LLSLFSLWL
Position: 115-123
Source molecule (GenBank): Q8IWU4.2
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PPI 2-10
Chemical type: Peptide from protein
Sequence: ALWMRLLPL
Position: 2-10
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PPI 2-10
Chemical type: Peptide from protein
Sequence: ALWMRLLPL
Position: 2-10
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PPI 15-24
Chemical type: Peptide from protein
Sequence: ALWGPDPAAA
Position: 15-24
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PPI 15-24
Chemical type: Peptide from protein
Sequence: ALWGPDPAAA
Position: 15-24
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PI B10-18 (PPI 34-42)
Chemical type: Peptide from protein
Sequence: HLVEALYLV
Position: 34-42
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: PI B10-18 (PPI 34-42)
Chemical type: Peptide from protein
Sequence: HLVEALYLV
Position: 34-42
Source molecule (GenBank): P01308.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: IAPP 5-13
Chemical type: Peptide from protein
Sequence: KLQVFLIVL
Position: 5-13
Source molecule (GenBank): P10997.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: IAPP 5-13
Chemical type: Peptide from protein
Sequence: KLQVFLIVL
Position: 5-13
Source molecule (GenBank): P10997.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: IA-2 206-214
Chemical type: Peptide from protein
Sequence: VIVMLTPLV
Position: 805-813
Source molecule (GenBank): Q16849.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: IA-2 206-214
Chemical type: Peptide from protein
Sequence: VIVMLTPLV
Position: 805-813
Source molecule (GenBank): Q16849.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GFAP 192-200
Chemical type: Peptide from protein
Sequence: SLEEEIRFL
Position: 192-200
Source molecule (GenBank): P14136.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GFAP 192-200
Chemical type: Peptide from protein
Sequence: SLEEEIRFL
Position: 192-200
Source molecule (GenBank): P14136.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GAD65 114-123
Chemical type: Peptide from protein
Sequence: VMNILLQYVV
Position: 114-123
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GAD65 114-123
Chemical type: Peptide from protein
Sequence: VMNILLQYVV
Position: 114-123
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo