Reference: Andrew D Redd; Alessandra Nardin; Hassen Kared; Evan M Bloch; Brian Abel; Andrew Pekosz; Oliver Laeyendecker; Michael Fehlings; Thomas C Quinn; Aaron A R Tobian. "Minimal Crossover between Mutations Associated with Omicron Variant of SARS-CoV-2 and CD8<sup>+</sup> T-Cell Epitopes Identified in COVID-19 Convalescent Individuals." mBio (2022).

Epitope: Replicase polyprotein 1ab 6429-6437
Chemical type: Peptide from protein
Sequence: AGFSLWVYK
Position: 6429-6437
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF3a protein 72-80
Chemical type: Peptide from protein
Sequence: ALSKGVHFV
Position: 72-80
Source molecule (GenBank): P0DTC3.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1a 4094-4102
Chemical type: Peptide from protein
Sequence: ALWEIQQVV
Position: 4094-4102
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Spike glycoprotein 1056-1063
Chemical type: Peptide from protein
Sequence: APHGVVFL
Position: 1056-1063
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1a 1176-1185
Chemical type: Peptide from protein
Sequence: AVFDKNLYDK
Position: 1176-1185
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1ab 5130-5138
Chemical type: Peptide from protein
Sequence: DTDFVNEFY
Position: 5130-5138
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF3a protein 207-215
Chemical type: Peptide from protein
Sequence: FTSDYYQLY
Position: 207-215
Source molecule (GenBank): P0DTC3.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1a 3437-3445
Chemical type: Peptide from protein
Sequence: GTDLEGNFY
Position: 3437-3445
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Spike glycoprotein 1099-1107
Chemical type: Peptide from protein
Sequence: GTHWFVTQR
Position: 1099-1107
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Spike glycoprotein 89-97
Chemical type: Peptide from protein
Sequence: GVYFASTEK
Position: 89-97
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Spike glycoprotein 89-97
Chemical type: Peptide from protein
Sequence: GVYFASTEK
Position: 89-97
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1a 1636-1646
Chemical type: Peptide from protein
Sequence: HTTDPSFLGRY
Position: 1636-1646
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1ab 5916-5924
Chemical type: Peptide from protein
Sequence: IPRRNVATL
Position: 5916-5924
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1ab 4673-4681
Chemical type: Peptide from protein
Sequence: KLFDRYFKY
Position: 4673-4681
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Nucleoprotein 361-369
Chemical type: Peptide from protein
Sequence: KTFPPTEPK
Position: 361-369
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Nucleoprotein 361-369
Chemical type: Peptide from protein
Sequence: KTFPPTEPK
Position: 361-369
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1a 282-290
Chemical type: Peptide from protein
Sequence: KTIQPRVEK
Position: 282-290
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1a 282-290
Chemical type: Peptide from protein
Sequence: KTIQPRVEK
Position: 282-290
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Nucleoprotein 222-230
Chemical type: Peptide from protein
Sequence: LLLDRLNQL
Position: 222-230
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF3a protein 139-147
Chemical type: Peptide from protein
Sequence: LLYDANYFL
Position: 139-147
Source molecule (GenBank): P0DTC3.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Spike glycoprotein 865-873
Chemical type: Peptide from protein
Sequence: LTDEMIAQY
Position: 865-873
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1ab 4456-4464
Chemical type: Peptide from protein
Sequence: NLIDSYFVV
Position: 4456-4464
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1a 2167-2175
Chemical type: Peptide from protein
Sequence: NYMPYFFTL
Position: 2167-2175
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Spike glycoprotein 448-456
Chemical type: Peptide from protein
Sequence: NYNYLYRLF
Position: 448-456
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1a 1321-1329
Chemical type: Peptide from protein
Sequence: PTDNYITTY
Position: 1321-1329
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1a 3370-3378
Chemical type: Peptide from protein
Sequence: QPGQTFSVL
Position: 3370-3378
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF7a protein 78-86
Chemical type: Peptide from protein
Sequence: RARSVSPKL
Position: 78-86
Source molecule (GenBank): P0DTC7.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Spike glycoprotein 78-86
Chemical type: Peptide from protein
Sequence: RFDNPVLPF
Position: 78-86
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1a 2949-2957
Chemical type: Peptide from protein
Sequence: RPDTRYVLM
Position: 2949-2957
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1a 3622-3630
Chemical type: Peptide from protein
Sequence: SAFAMMFVK
Position: 3622-3630
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF3a protein 58-66
Chemical type: Peptide from protein
Sequence: SASKIITLK
Position: 58-66
Source molecule (GenBank): P0DTC3.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF3a protein 58-66
Chemical type: Peptide from protein
Sequence: SASKIITLK
Position: 58-66
Source molecule (GenBank): P0DTC3.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Nucleoprotein 105-113
Chemical type: Peptide from protein
Sequence: SPRWYFYYL
Position: 105-113
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1a 2600-2608
Chemical type: Peptide from protein
Sequence: STFNVPMEK
Position: 2600-2608
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1a 1504-1512
Chemical type: Peptide from protein
Sequence: TISLAGSYK
Position: 1504-1512
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1a 1504-1512
Chemical type: Peptide from protein
Sequence: TISLAGSYK
Position: 1504-1512
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1ab 4716-4724
Chemical type: Peptide from protein
Sequence: TSFGPLVRK
Position: 4716-4724
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1ab 6148-6156
Chemical type: Peptide from protein
Sequence: TYACWHHSI
Position: 6148-6156
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1a 808-816
Chemical type: Peptide from protein
Sequence: VTNNTFTLK
Position: 808-816
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1a 808-816
Chemical type: Peptide from protein
Sequence: VTNNTFTLK
Position: 808-816
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Spike glycoprotein 1060-1068
Chemical type: Peptide from protein
Sequence: VVFLHVTYV
Position: 1060-1068
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1ab 5710-5718
Chemical type: Peptide from protein
Sequence: VVNARLRAK
Position: 5710-5718
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1ab 5533-5542
Chemical type: Peptide from protein
Sequence: VVYRGTTTYK
Position: 5533-5542
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF3a protein 112-120
Chemical type: Peptide from protein
Sequence: VYFLQSINF
Position: 112-120
Source molecule (GenBank): P0DTC3.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1ab 5721-5729
Chemical type: Peptide from protein
Sequence: VYIGDPAQL
Position: 5721-5729
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1a 1675-1683
Chemical type: Peptide from protein
Sequence: YLATALLTL
Position: 1675-1683
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1ab 6419-6427
Chemical type: Peptide from protein
Sequence: YLDAYNMMI
Position: 6419-6427
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Spike glycoprotein 269-277
Chemical type: Peptide from protein
Sequence: YLQPRTFLL
Position: 269-277
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF3a protein 107-115
Chemical type: Peptide from protein
Sequence: YLYALVYFL
Position: 107-115
Source molecule (GenBank): P0DTC3.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: Replicase polyprotein 1a 1536-1544
Chemical type: Peptide from protein
Sequence: YYTSNPTTF
Position: 1536-1544
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo