Reference: Tanner M Johanns; James M Ertelt; Joseph C Lai; Jared H Rowe; Ross A Avant; Sing Sing Way. "Naturally occurring altered peptide ligands control Salmonella-specific CD4+ T cell proliferation, IFN-gamma production, and protective potency." Journal of immunology (Baltimore, Md. : 1950) (2010).

Epitope: FliC<sub>431-439</sub>
Chemical type: Peptide from protein
Sequence: FNSAITNLG
Position: 433-441
Source molecule (GenBank): P06179.4
Assay category: TCell
Result: Positive
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: FliC<sub>431-439</sub>
Chemical type: Peptide from protein
Sequence: FNSAITNLG
Position: 433-441
Source molecule (GenBank): P06179.4
Assay category: TCell
Result: Positive
Host:  6-8 weeks

Epitope: FliC<sub>431-439</sub>
Chemical type: Peptide from protein
Sequence: FNSAITNLG
Position: 433-441
Source molecule (GenBank): P06179.4
Assay category: TCell
Result: Positive-Intermediate
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: FliC<sub>431-439</sub>
Chemical type: Peptide from protein
Sequence: FNSAITNLG
Position: 433-441
Source molecule (GenBank): P06179.4
Assay category: TCell
Result: Positive
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: FliC<sub>L438I</sub>
Chemical type: Peptide from protein
Sequence: FNSAITNIG
Position: 444-452
Source molecule (GenBank): AAR10739.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks

Epitope: FliC<sub>L438I</sub>
Chemical type: Peptide from protein
Sequence: FNSAITNIG
Position: 444-452
Source molecule (GenBank): AAR10739.1
Assay category: TCell
Result: Positive-High
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: FliC<sub>L438I</sub>
Chemical type: Peptide from protein
Sequence: FNSAITNIG
Position: 444-452
Source molecule (GenBank): AAR10739.1
Assay category: TCell
Result: Positive
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: FliC<sub>L438I</sub>
Chemical type: Peptide from protein
Sequence: FNSAITNIG
Position: 444-452
Source molecule (GenBank): AAR10739.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: FliC<sub>N432D</sub>
Chemical type: Peptide from protein
Sequence: FDSAITNLG
Position: 394-402
Source molecule (GenBank): ZP_03341484.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: FliC<sub>N432D</sub>
Chemical type: Peptide from protein
Sequence: FDSAITNLG
Position: 394-402
Source molecule (GenBank): ZP_03341484.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: FliC<sub>N432D</sub>
Chemical type: Peptide from protein
Sequence: FDSAITNLG
Position: 394-402
Source molecule (GenBank): ZP_03341484.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: FliC<sub>N432D</sub>
Chemical type: Peptide from protein
Sequence: FDSAITNLG
Position: 394-402
Source molecule (GenBank): ZP_03341484.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks

Epitope: FliC<sub>N432E</sub>
Chemical type: Peptide from protein
Sequence: FESAITNLG
Position: 334-342
Source molecule (GenBank): ABJ99885.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: FliC<sub>N432E</sub>
Chemical type: Peptide from protein
Sequence: FESAITNLG
Position: 334-342
Source molecule (GenBank): ABJ99885.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: FliC<sub>S433F</sub>
Chemical type: Peptide from protein
Sequence: FNFAITNLG
Position: 423-431
Source molecule (GenBank): ABI96368.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: FliC<sub>S433F</sub>
Chemical type: Peptide from protein
Sequence: FNFAITNLG
Position: 423-431
Source molecule (GenBank): ABI96368.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo