Reference: Hsin-Wei Chen; Hui-Mei Hu; Szu-Hsien Wu; Chen-Yi Chiang; Yu-Ju Hsiao; Chia-Kai Wu; Chun-Hsiang Hsieh; Han-Hsuan Chung; Pele Chong; Chih-Hsiang Leng; Chien-Hsiung Pan. "The Immunodominance Change and Protection of CD4+ T-Cell Responses Elicited by an Envelope Protein Domain III-Based Tetravalent Dengue Vaccine in Mice." PloS one (2015).

Epitope: D1-10
Chemical type: Peptide from protein
Sequence: GRLITANPIVTDKEK
Position: 349-363
Source molecule (GenBank): AAF03887.1
Assay category: TCell
Result: Positive
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D2-10
Chemical type: Peptide from protein
Sequence: GRLITVNPIVTEKDS
Position: 63-77
Source molecule (GenBank): AGG79337.1
Assay category: TCell
Result: Positive
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D3-10
Chemical type: Peptide from protein
Sequence: GRLITANPVVTKKEE
Position: 230-244
Source molecule (GenBank): ABA25784.1
Assay category: TCell
Result: Positive
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D4-10
Chemical type: Peptide from protein
Sequence: GRIISSTPFAENTNS
Position: 58-72
Source molecule (GenBank): AGW23582.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D1-4
Chemical type: Peptide from protein
Sequence: AETQHGTVLVQVKYE
Position: 313-327
Source molecule (GenBank): AAN32773.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D2-4
Chemical type: Peptide from protein
Sequence: AETQHGTIVIRVQYE
Position: 313-327
Source molecule (GenBank): AAF61121.1
Assay category: TCell
Result: Positive
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D3-4
Chemical type: Peptide from protein
Sequence: SETQHGTILIKVEYK
Position: 194-208
Source molecule (GenBank): ABA25784.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D4-4
Chemical type: Peptide from protein
Sequence: AETQHGTTVVKVKYE
Position: 22-36
Source molecule (GenBank): AGW23582.1
Assay category: TCell
Result: Positive
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D1-1
Chemical type: Peptide from protein
Sequence: KGMSYVMCTGSFKLE
Position: 295-309
Source molecule (GenBank): AAN32773.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D1-2
Chemical type: Peptide from protein
Sequence: MCTGSFKLEKEVAET
Position: 301-315
Source molecule (GenBank): AAF03887.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D1-3
Chemical type: Peptide from protein
Sequence: KLEKEVAETQHGTVL
Position: 307-321
Source molecule (GenBank): AAN32773.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D1-5
Chemical type: Peptide from protein
Sequence: TVLVQVKYEGTDAPC
Position: 319-333
Source molecule (GenBank): AAN32773.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D1-6
Chemical type: Peptide from protein
Sequence: KYEGTDAPCKIPFSS
Position: 325-339
Source molecule (GenBank): AAF03887.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D1-8
Chemical type: Peptide from protein
Sequence: FSSQDEKGVTQNGRL
Position: 337-351
Source molecule (GenBank): AAF03887.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D1-9
Chemical type: Peptide from protein
Sequence: KGVTQNGRLITANPI
Position: 343-357
Source molecule (GenBank): AAF03887.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D1-11
Chemical type: Peptide from protein
Sequence: NPIVTDKEKPVNIEA
Position: 355-369
Source molecule (GenBank): AAN32773.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D1-12
Chemical type: Peptide from protein
Sequence: KEKPVNIEAEPPFGE
Position: 361-375
Source molecule (GenBank): AAF03887.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D1-13
Chemical type: Peptide from protein
Sequence: IEAEPPFGESYIVVG
Position: 367-381
Source molecule (GenBank): AAF03887.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D1-15
Chemical type: Peptide from protein
Sequence: VVGAGEKALKLSWFK
Position: 379-393
Source molecule (GenBank): AAZ43213.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D1-16
Chemical type: Peptide from protein
Sequence: KALKLSWFKKGSS
Position: 385-397
Source molecule (GenBank): AAF03887.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D2-3
Chemical type: Peptide from protein
Sequence: KVVKEIAETQHGTIV
Position: 307-321
Source molecule (GenBank): AAF61121.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D2-5
Chemical type: Peptide from protein
Sequence: TIVIRVQYEGDGSPC
Position: 319-333
Source molecule (GenBank): AAM75194.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D2-6
Chemical type: Peptide from protein
Sequence: QYEGDGSPCKIPFEI
Position: 325-339
Source molecule (GenBank): AAM75194.1
Assay category: TCell
Result: Positive
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D2-8
Chemical type: Peptide from protein
Sequence: FEIMDLEKRHVLGRL
Position: 337-351
Source molecule (GenBank): BAC77256.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D2-9
Chemical type: Peptide from protein
Sequence: EKRHVLGRLITVNPI
Position: 343-357
Source molecule (GenBank): BAC77256.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D2-11
Chemical type: Peptide from protein
Sequence: NPIVTEKDSPVNIEA
Position: 355-369
Source molecule (GenBank): AAM75194.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D2-12
Chemical type: Peptide from protein
Sequence: KDSPVNIEAEPPFGD
Position: 361-375
Source molecule (GenBank): AAM75194.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D2-13
Chemical type: Peptide from protein
Sequence: IEAEPPFGDSYIIIG
Position: 367-381
Source molecule (GenBank): AAM75194.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D2-14
Chemical type: Peptide from protein
Sequence: FGDSYIIIGVEPGQL
Position: 373-387
Source molecule (GenBank): AAM75194.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D2-15
Chemical type: Peptide from protein
Sequence: IIGVEPGQLKLNWFK
Position: 379-393
Source molecule (GenBank): AAS49651.3
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D2-16
Chemical type: Peptide from protein
Sequence: GQLKLNWFKKGSS
Position: 385-397
Source molecule (GenBank): AAS49651.3
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D3-2
Chemical type: Peptide from protein
Sequence: MCLNTFVLKKEVSET
Position: 299-313
Source molecule (GenBank): BAC77233.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D3-3
Chemical type: Peptide from protein
Sequence: VLKKEVSETQHGTIL
Position: 305-319
Source molecule (GenBank): BAC77233.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D3-5
Chemical type: Peptide from protein
Sequence: TILIKVEYKGEDAPC
Position: 317-331
Source molecule (GenBank): BAC77233.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D3-6
Chemical type: Peptide from protein
Sequence: EYKGEDAPCKIPFST
Position: 323-337
Source molecule (GenBank): BAC77233.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D3-7
Chemical type: Peptide from protein
Sequence: APCKIPFSTEDGQGK
Position: 329-343
Source molecule (GenBank): BAC77233.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D3-8
Chemical type: Peptide from protein
Sequence: FSTEDGQGKAHNGRL
Position: 335-349
Source molecule (GenBank): BAC77233.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D3-9
Chemical type: Peptide from protein
Sequence: QGKAHNGRLITANPV
Position: 341-355
Source molecule (GenBank): BAC77233.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D3-11
Chemical type: Peptide from protein
Sequence: NPVVTKKEEPVNIEA
Position: 353-367
Source molecule (GenBank): BAC77233.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D3-12
Chemical type: Peptide from protein
Sequence: KEEPVNIEAEPPFGE
Position: 359-373
Source molecule (GenBank): BAC77233.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D3-13
Chemical type: Peptide from protein
Sequence: IEAEPPFGESNIVIG
Position: 365-379
Source molecule (GenBank): BAC77233.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D3-14
Chemical type: Peptide from protein
Sequence: FGESNIVIGIGDKAL
Position: 371-385
Source molecule (GenBank): BAC77233.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D3-15
Chemical type: Peptide from protein
Sequence: VIGIGDKALKINWYK
Position: 260-274
Source molecule (GenBank): ABA25784.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D3-16
Chemical type: Peptide from protein
Sequence: KALKINWYKKGSS
Position: 266-278
Source molecule (GenBank): ABA25784.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D4-1
Chemical type: Peptide from protein
Sequence: KGMSYTMCSGKFSID
Position: 295-309
Source molecule (GenBank): AAF61130.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D4-2
Chemical type: Peptide from protein
Sequence: MCSGKFSIDKEMAET
Position: 301-315
Source molecule (GenBank): AAF61130.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D4-3
Chemical type: Peptide from protein
Sequence: SIDKEMAETQHGTTV
Position: 307-321
Source molecule (GenBank): AAF61130.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D4-5
Chemical type: Peptide from protein
Sequence: TTVVKVKYEGAGAPC
Position: 319-333
Source molecule (GenBank): AAF61130.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D4-6
Chemical type: Peptide from protein
Sequence: KYEGAGAPCKVPIEI
Position: 325-339
Source molecule (GenBank): AAF61130.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D4-7
Chemical type: Peptide from protein
Sequence: APCKVPIEIRDVNKE
Position: 331-345
Source molecule (GenBank): AAF61130.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D4-8
Chemical type: Peptide from protein
Sequence: IEIRDVNKEKVVGRI
Position: 337-351
Source molecule (GenBank): AAF61130.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D4-9
Chemical type: Peptide from protein
Sequence: NKEKVVGRIISSTPF
Position: 343-357
Source molecule (GenBank): ACC68765.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D4-12
Chemical type: Peptide from protein
Sequence: TNSVTNIELEPPFGD
Position: 361-375
Source molecule (GenBank): AAF61130.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D4-13
Chemical type: Peptide from protein
Sequence: IELEPPFGDSYIVIG
Position: 367-381
Source molecule (GenBank): AAF61130.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D4-14
Chemical type: Peptide from protein
Sequence: FGDSYIVIGVGDSAL
Position: 373-387
Source molecule (GenBank): AAU89366.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D4-15
Chemical type: Peptide from protein
Sequence: VIGVGDSALTLHWFR
Position: 379-393
Source molecule (GenBank): AAU89366.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D4-16
Chemical type: Peptide from protein
Sequence: SALTLHWFRKGSS
Position: 385-397
Source molecule (GenBank): AAF61130.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo