Reference: E A James; W W Kwok; R A Ettinger; A R Thompson; K P Pratt. "T-cell responses over time in a mild hemophilia A inhibitor subject: epitope identification and transient immunogenicity of the corresponding self-peptide." Journal of thrombosis and haemostasis : JTH (2007).

Epitope: FVIII<sub>484-501</sub>
Chemical type: Peptide from protein
Sequence: RPLYSRRLPKGVKHLKDFPILPGEI
Position: 503-527
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 33 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>484-501</sub>
Chemical type: Peptide from protein
Sequence: RPLYSRRLPKGVKHLKDFPILPGEI
Position: 503-527
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Positive
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>484-501</sub>
Chemical type: Peptide from protein
Sequence: RPLYSRRLPKGVKHLKDFPILPGEI
Position: 503-527
Source molecule (GenBank): P00451.1
Assay category: MhcBinding
Result: Negative

Epitope: FVIII<sub>519-538</sub>
Chemical type: Peptide from protein
Sequence: DGPTKSDPRCLTRYYSSFVN
Position: 538-557
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>519-538</sub>
Chemical type: Peptide from protein
Sequence: DGPTKSDPRCLTRYYSSFVN
Position: 538-557
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 33 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2170-2189</sub>
Chemical type: Peptide from protein
Sequence: DLNSCSMPLGMESKAISDAQ
Position: 2189-2208
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2170-2189</sub>
Chemical type: Peptide from protein
Sequence: DLNSCSMPLGMESKAISDAQ
Position: 2189-2208
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 33 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2178-2197</sub>
Chemical type: Peptide from protein
Sequence: LGMESKAISDAQITASSYFT
Position: 2197-2216
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 33 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2178-2197</sub>
Chemical type: Peptide from protein
Sequence: LGMESKAISDAQITASSYFT
Position: 2197-2216
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2187-2205</sub>
Chemical type: Peptide from protein
Sequence: DAQITASSYFTNMFATWSP
Position: 2206-2224
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Positive
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2187-2205</sub>
Chemical type: Peptide from protein
Sequence: DAQITASSYFTNMFATWSP
Position: 2206-2224
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 33 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2187-2205</sub>
Chemical type: Peptide from protein
Sequence: DAQITASSYFTNMFATWSP
Position: 2206-2224
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Positive
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: FVIII<sub>2186-2205</sub>
Chemical type: Peptide from protein
Sequence: SDAQITASSYFTNMFATWSP
Position: 2205-2224
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Positive
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2186-2205</sub>
Chemical type: Peptide from protein
Sequence: SDAQITASSYFTNMFATWSP
Position: 2205-2224
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 33 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2194-2213</sub>
Chemical type: Peptide from protein
Sequence: SYFTNMFATWSPSKARLHLQ
Position: 2213-2232
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Positive
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: FVIII<sub>2194-2213</sub>
Chemical type: Peptide from protein
Sequence: SYFTNMFATWSPSKARLHLQ
Position: 2213-2232
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 33 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2194-2213</sub>
Chemical type: Peptide from protein
Sequence: SYFTNMFATWSPSKARLHLQ
Position: 2213-2232
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Positive
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: FVIII<sub>2194-2213</sub>
Chemical type: Peptide from protein
Sequence: SYFTNMFATWSPSKARLHLQ
Position: 2213-2232
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Positive
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: FVIII<sub>2194-2213</sub>
Chemical type: Peptide from protein
Sequence: SYFTNMFATWSPSKARLHLQ
Position: 2213-2232
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Positive
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: FVIII<sub>2194-2213</sub>
Chemical type: Peptide from protein
Sequence: SYFTNMFATWSPSKARLHLQ
Position: 2213-2232
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Positive
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: FVIII<sub>2194-2213</sub>
Chemical type: Peptide from protein
Sequence: SYFTNMFATWSPSKARLHLQ
Position: 2213-2232
Source molecule (GenBank): P00451.1
Assay category: MhcBinding
Result: Positive

Epitope: FVIII<sub>2202-2221</sub>
Chemical type: Peptide from protein
Sequence: TWSPSKARLHLQGRSNAWRP
Position: 2221-2240
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2202-2221</sub>
Chemical type: Peptide from protein
Sequence: TWSPSKARLHLQGRSNAWRP
Position: 2221-2240
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 33 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2210-2229</sub>
Chemical type: Peptide from protein
Sequence: LHLQGRSNAWRPQVNNPKEW
Position: 2229-2248
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 33 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2210-2229</sub>
Chemical type: Peptide from protein
Sequence: LHLQGRSNAWRPQVNNPKEW
Position: 2229-2248
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2218-2237</sub>
Chemical type: Peptide from protein
Sequence: AWRPQVNNPKEWLQVDFQKT
Position: 2237-2256
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2218-2237</sub>
Chemical type: Peptide from protein
Sequence: AWRPQVNNPKEWLQVDFQKT
Position: 2237-2256
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 33 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2226-2245</sub>
Chemical type: Peptide from protein
Sequence: PKEWLQVDFQKTMKVTGVTT
Position: 2245-2264
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2226-2245</sub>
Chemical type: Peptide from protein
Sequence: PKEWLQVDFQKTMKVTGVTT
Position: 2245-2264
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 33 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2234-2253</sub>
Chemical type: Peptide from protein
Sequence: FQKTMKVTGVTTQGVKSLLT
Position: 2253-2272
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 33 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2234-2253</sub>
Chemical type: Peptide from protein
Sequence: FQKTMKVTGVTTQGVKSLLT
Position: 2253-2272
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2242-2261</sub>
Chemical type: Peptide from protein
Sequence: GVTTQGVKSLLTSMYVKEFL
Position: 2261-2280
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2242-2261</sub>
Chemical type: Peptide from protein
Sequence: GVTTQGVKSLLTSMYVKEFL
Position: 2261-2280
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 33 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2250-2269</sub>
Chemical type: Peptide from protein
Sequence: SLLTSMYVKEFLISSSQDGH
Position: 2269-2288
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2250-2269</sub>
Chemical type: Peptide from protein
Sequence: SLLTSMYVKEFLISSSQDGH
Position: 2269-2288
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 33 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2258-2277</sub>
Chemical type: Peptide from protein
Sequence: KEFLISSSQDGHQWTLFFQN
Position: 2277-2296
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2258-2277</sub>
Chemical type: Peptide from protein
Sequence: KEFLISSSQDGHQWTLFFQN
Position: 2277-2296
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 33 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2273-2292</sub>
Chemical type: Peptide from protein
Sequence: LFFQNGKVKVFQGNQDSFTP
Position: 2292-2311
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2273-2292</sub>
Chemical type: Peptide from protein
Sequence: LFFQNGKVKVFQGNQDSFTP
Position: 2292-2311
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 33 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2281-2300</sub>
Chemical type: Peptide from protein
Sequence: KVFQGNQDSFTPVVNSLDPP
Position: 2300-2319
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2281-2300</sub>
Chemical type: Peptide from protein
Sequence: KVFQGNQDSFTPVVNSLDPP
Position: 2300-2319
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 33 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2289-2308</sub>
Chemical type: Peptide from protein
Sequence: SFTPVVNSLDPPLLTRYLRI
Position: 2308-2327
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 33 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2289-2308</sub>
Chemical type: Peptide from protein
Sequence: SFTPVVNSLDPPLLTRYLRI
Position: 2308-2327
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2297-2316</sub>
Chemical type: Peptide from protein
Sequence: LDPPLLTRYLRIHPQSWVHQ
Position: 2316-2335
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 33 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2297-2316</sub>
Chemical type: Peptide from protein
Sequence: LDPPLLTRYLRIHPQSWVHQ
Position: 2316-2335
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2305-2324</sub>
Chemical type: Peptide from protein
Sequence: YLRIHPQSWVHQIALRMEVL
Position: 2324-2343
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 33 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2305-2324</sub>
Chemical type: Peptide from protein
Sequence: YLRIHPQSWVHQIALRMEVL
Position: 2324-2343
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2313-2332</sub>
Chemical type: Peptide from protein
Sequence: WVHQIALRMEVLGCEAQDLY
Position: 2332-2351
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 33 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2313-2332</sub>
Chemical type: Peptide from protein
Sequence: WVHQIALRMEVLGCEAQDLY
Position: 2332-2351
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2317-2332</sub>
Chemical type: Peptide from protein
Sequence: IALRMEVLGCEAQDLY
Position: 2336-2351
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2317-2332</sub>
Chemical type: Peptide from protein
Sequence: IALRMEVLGCEAQDLY
Position: 2336-2351
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host: M 33 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2194-2213, 2201P</sub>
Chemical type: Peptide from protein
Sequence: SYFTNMFPTWSPSKARLHLQ
Assay category: TCell
Result: Negative
Host: M 33 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2194-2213, 2201P</sub>
Chemical type: Peptide from protein
Sequence: SYFTNMFPTWSPSKARLHLQ
Assay category: TCell
Result: Negative
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2194-2213, 2201P</sub>
Chemical type: Peptide from protein
Sequence: SYFTNMFPTWSPSKARLHLQ
Assay category: TCell
Result: Positive
Host: M 20 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: FVIII<sub>2194-2213, 2201P</sub>
Chemical type: Peptide from protein
Sequence: SYFTNMFPTWSPSKARLHLQ
Assay category: MhcBinding
Result: Positive-Low