Reference: John W McGinty; I-Ting Chow; Carla Greenbaum; Jared Odegard; William W Kwok; Eddie A James. "Recognition of posttranslationally modified GAD65 epitopes in subjects with type 1 diabetes." Diabetes (2014).

Epitope: GAD65 89-108 105Cit
Chemical type: Peptide from protein
Sequence: YAFLHATDLLPACDGERPTL
Position: 89-108
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Host:  10-63 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 89-108 105Cit
Chemical type: Peptide from protein
Sequence: YAFLHATDLLPACDGERPTL
Position: 89-108
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Host:  10-63 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GAD65 89-108 105Cit
Chemical type: Peptide from protein
Sequence: YAFLHATDLLPACDGERPTL
Position: 89-108
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 2900.0

Epitope: GAD65 153–172 154E,164E
Chemical type: Peptide from protein
Sequence: PQNLEEILMHCQTTLKYAIK
Position: 153-172
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Host:  10-63 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GAD65 153–172 154E,164E
Chemical type: Peptide from protein
Sequence: PQNLEEILMHCQTTLKYAIK
Position: 153-172
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Host:  10-63 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 153–172 154E,164E
Chemical type: Peptide from protein
Sequence: PQNLEEILMHCQTTLKYAIK
Position: 153-172
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 12800.0

Epitope: GAD65 265–284 272Cit
Chemical type: Peptide from protein
Sequence: KGMAALPRLIAFTSEHSHFS
Position: 265-284
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Host:  10-63 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GAD65 265–284 272Cit
Chemical type: Peptide from protein
Sequence: KGMAALPRLIAFTSEHSHFS
Position: 265-284
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Negative
Host:  20-61 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GAD65 265–284 272Cit
Chemical type: Peptide from protein
Sequence: KGMAALPRLIAFTSEHSHFS
Position: 265-284
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Host:  10-63 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GAD65 265–284 272Cit
Chemical type: Peptide from protein
Sequence: KGMAALPRLIAFTSEHSHFS
Position: 265-284
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Host:  10-63 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GAD65 265–284 272Cit
Chemical type: Peptide from protein
Sequence: KGMAALPRLIAFTSEHSHFS
Position: 265-284
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Host:  10-63 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GAD65 265–284 272Cit
Chemical type: Peptide from protein
Sequence: KGMAALPRLIAFTSEHSHFS
Position: 265-284
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive-Low
Host:  19-54 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GAD65 265–284 272Cit
Chemical type: Peptide from protein
Sequence: KGMAALPRLIAFTSEHSHFS
Position: 265-284
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 800.0

Epitope: GAD65 553–572 558Cit
Chemical type: Peptide from protein
Sequence: KVNFFRMVISNPAATHQDID
Position: 553-572
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Host:  10-63 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GAD65 553–572 558Cit
Chemical type: Peptide from protein
Sequence: KVNFFRMVISNPAATHQDID
Position: 553-572
Source molecule (GenBank): Q05329.1
Assay category: TCell
Result: Positive
Host:  10-63 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GAD65 553–572 558Cit
Chemical type: Peptide from protein
Sequence: KVNFFRMVISNPAATHQDID
Position: 553-572
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 40.0

Epitope: GAD65 89-108
Chemical type: Peptide from protein
Sequence: YAFLHATDLLPACDGERPTL
Position: 89-108
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 3200.0

Epitope: GAD65 115–127
Chemical type: Peptide from protein
Sequence: MNILLQYVVKSFD
Position: 115-127
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 700.0

Epitope: GAD65 153–172
Chemical type: Peptide from protein
Sequence: PQNLEEILMHCQTTLKYAIK
Position: 153-172
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 5600.0

Epitope: GAD65 73‐92 77E
Chemical type: Peptide from protein
Sequence: CACDQKPCSCSKVDVNYAFL
Position: 73-92
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 5020.0

Epitope: GAD65 177‐196 181E
Chemical type: Peptide from protein
Sequence: RYFNQLSTGLDMVGLAADWL
Position: 177-196
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 2700.0

Epitope: GAD65 321‐340 324E
Chemical type: Peptide from protein
Sequence: EAKQKGFVPFLVSATAGTTV
Position: 321-340
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 5090.0

Epitope: GAD65 377‐396 386Cit
Chemical type: Peptide from protein
Sequence: HKWKLSGVERANSVTWNPHK
Position: 377-396
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 7940.0

Epitope: GAD65 441‐456 448Cit
Chemical type: Peptide from protein
Sequence: DKALQCGRHVDVFKLWLMW
Position: 441-459
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 20550.0

Epitope: GAD65 473‐492 488Cit
Chemical type: Peptide from protein
Sequence: KCLELAEYLYNIIKNREGYE
Position: 473-492
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 890.0

Epitope: GAD65 481‐500 488Cit
Chemical type: Peptide from protein
Sequence: LYNIIKNREGYEMVFDGKPQ
Position: 481-500
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 3810.0

Epitope: GAD65 505‐524 514Cit, 522Cit
Chemical type: Peptide from protein
Sequence: CFWYIPPSLRTLEDNEERMS
Position: 505-524
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 3300.0

Epitope: GAD65 529‐548 536Cit
Chemical type: Peptide from protein
Sequence: VAPVIKARMMEYGTTMVSYQ
Position: 529-548
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 5230.0

Epitope: GAD65 545‐564 558Cit
Chemical type: Peptide from protein
Sequence: VSYQPLGDKVNFFRMVISNP
Position: 545-564
Source molecule (GenBank): Q05329.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 8070.0