Reference: Ana M Espino; Daricel Torres; Adelaida Morales; Bonnibel Delgado; Julia Quetel; Antonio Osuna. "Fasciola hepatica: identification of CD4+ T-helper epitopes from the 11.5 kDa saposin-like protein SAP-2 using synthetic peptides." Experimental parasitology (2007).

Epitope: P3
Chemical type: Peptide from protein
Sequence: AVTFASFDVPSKQPT
Position: 11-25
Source molecule (GenBank): AAF88069.1
Assay category: BCell
Result: Positive
Host: F 6-8 weeks

Epitope: P3
Chemical type: Peptide from protein
Sequence: AVTFASFDVPSKQPT
Position: 11-25
Source molecule (GenBank): AAF88069.1
Assay category: BCell
Result: Positive
Host: F 6-8 weeks

Epitope: P3
Chemical type: Peptide from protein
Sequence: AVTFASFDVPSKQPT
Position: 11-25
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P3
Chemical type: Peptide from protein
Sequence: AVTFASFDVPSKQPT
Position: 11-25
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P3
Chemical type: Peptide from protein
Sequence: AVTFASFDVPSKQPT
Position: 11-25
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P3
Chemical type: Peptide from protein
Sequence: AVTFASFDVPSKQPT
Position: 11-25
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P3
Chemical type: Peptide from protein
Sequence: AVTFASFDVPSKQPT
Position: 11-25
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P4
Chemical type: Peptide from protein
Sequence: SFDVPSKQPTIDIDL
Position: 16-30
Source molecule (GenBank): AAF88069.1
Assay category: BCell
Result: Positive
Host: F 6-8 weeks

Epitope: P4
Chemical type: Peptide from protein
Sequence: SFDVPSKQPTIDIDL
Position: 16-30
Source molecule (GenBank): AAF88069.1
Assay category: BCell
Result: Positive
Host: F 6-8 weeks

Epitope: P4
Chemical type: Peptide from protein
Sequence: SFDVPSKQPTIDIDL
Position: 16-30
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P4
Chemical type: Peptide from protein
Sequence: SFDVPSKQPTIDIDL
Position: 16-30
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P4
Chemical type: Peptide from protein
Sequence: SFDVPSKQPTIDIDL
Position: 16-30
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P4
Chemical type: Peptide from protein
Sequence: SFDVPSKQPTIDIDL
Position: 16-30
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P4
Chemical type: Peptide from protein
Sequence: SFDVPSKQPTIDIDL
Position: 16-30
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P6
Chemical type: Peptide from protein
Sequence: IDIDLCDICTNTMDV
Position: 26-40
Source molecule (GenBank): AAF88069.1
Assay category: BCell
Result: Positive
Host: F 6-8 weeks

Epitope: P6
Chemical type: Peptide from protein
Sequence: IDIDLCDICTNTMDV
Position: 26-40
Source molecule (GenBank): AAF88069.1
Assay category: BCell
Result: Negative
Host: F 6-8 weeks

Epitope: P6
Chemical type: Peptide from protein
Sequence: IDIDLCDICTNTMDV
Position: 26-40
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P6
Chemical type: Peptide from protein
Sequence: IDIDLCDICTNTMDV
Position: 26-40
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P6
Chemical type: Peptide from protein
Sequence: IDIDLCDICTNTMDV
Position: 26-40
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P6
Chemical type: Peptide from protein
Sequence: IDIDLCDICTNTMDV
Position: 26-40
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P6
Chemical type: Peptide from protein
Sequence: IDIDLCDICTNTMDV
Position: 26-40
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P7
Chemical type: Peptide from protein
Sequence: CDICTNTMDVIKKML
Position: 31-45
Source molecule (GenBank): AAF88069.1
Assay category: BCell
Result: Positive
Host: F 6-8 weeks

Epitope: P7
Chemical type: Peptide from protein
Sequence: CDICTNTMDVIKKML
Position: 31-45
Source molecule (GenBank): AAF88069.1
Assay category: BCell
Result: Negative
Host: F 6-8 weeks

Epitope: P7
Chemical type: Peptide from protein
Sequence: CDICTNTMDVIKKML
Position: 31-45
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P7
Chemical type: Peptide from protein
Sequence: CDICTNTMDVIKKML
Position: 31-45
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P7
Chemical type: Peptide from protein
Sequence: CDICTNTMDVIKKML
Position: 31-45
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P7
Chemical type: Peptide from protein
Sequence: CDICTNTMDVIKKML
Position: 31-45
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P8
Chemical type: Peptide from protein
Sequence: NTMDVIKKMLADQTV
Position: 36-50
Source molecule (GenBank): AAF88069.1
Assay category: BCell
Result: Positive
Host: F 6-8 weeks

Epitope: P8
Chemical type: Peptide from protein
Sequence: NTMDVIKKMLADQTV
Position: 36-50
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P9
Chemical type: Peptide from protein
Sequence: IKKMLADQTVEEHIG
Position: 41-55
Source molecule (GenBank): AAF88069.1
Assay category: BCell
Result: Positive
Host: F 6-8 weeks

Epitope: P9
Chemical type: Peptide from protein
Sequence: IKKMLADQTVEEHIG
Position: 41-55
Source molecule (GenBank): AAF88069.1
Assay category: BCell
Result: Positive
Host: F 6-8 weeks

Epitope: P9
Chemical type: Peptide from protein
Sequence: IKKMLADQTVEEHIG
Position: 41-55
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P9
Chemical type: Peptide from protein
Sequence: IKKMLADQTVEEHIG
Position: 41-55
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P9
Chemical type: Peptide from protein
Sequence: IKKMLADQTVEEHIG
Position: 41-55
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P9
Chemical type: Peptide from protein
Sequence: IKKMLADQTVEEHIG
Position: 41-55
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P10
Chemical type: Peptide from protein
Sequence: ADQTVEEHIGYLVKY
Position: 46-60
Source molecule (GenBank): AAF88069.1
Assay category: BCell
Result: Positive
Host: F 6-8 weeks

Epitope: P10
Chemical type: Peptide from protein
Sequence: ADQTVEEHIGYLVKY
Position: 46-60
Source molecule (GenBank): AAF88069.1
Assay category: BCell
Result: Positive
Host: F 6-8 weeks

Epitope: P10
Chemical type: Peptide from protein
Sequence: ADQTVEEHIGYLVKY
Position: 46-60
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P10
Chemical type: Peptide from protein
Sequence: ADQTVEEHIGYLVKY
Position: 46-60
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P10
Chemical type: Peptide from protein
Sequence: ADQTVEEHIGYLVKY
Position: 46-60
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P10
Chemical type: Peptide from protein
Sequence: ADQTVEEHIGYLVKY
Position: 46-60
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P10
Chemical type: Peptide from protein
Sequence: ADQTVEEHIGYLVKY
Position: 46-60
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P11
Chemical type: Peptide from protein
Sequence: EEHIGYLVKYLCKIA
Position: 51-65
Source molecule (GenBank): AAF88069.1
Assay category: BCell
Result: Negative
Host: F 6-8 weeks

Epitope: P11
Chemical type: Peptide from protein
Sequence: EEHIGYLVKYLCKIA
Position: 51-65
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P12
Chemical type: Peptide from protein
Sequence: YLVKYLCKIARSQDA
Position: 56-70
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P13
Chemical type: Peptide from protein
Sequence: LCKIARSQDACIEFV
Position: 61-75
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P14
Chemical type: Peptide from protein
Sequence: RSQDACIEFVQQEVD
Position: 66-80
Source molecule (GenBank): AAF88069.1
Assay category: BCell
Result: Positive
Host: F 6-8 weeks

Epitope: P14
Chemical type: Peptide from protein
Sequence: RSQDACIEFVQQEVD
Position: 66-80
Source molecule (GenBank): AAF88069.1
Assay category: BCell
Result: Negative
Host: F 6-8 weeks

Epitope: P14
Chemical type: Peptide from protein
Sequence: RSQDACIEFVQQEVD
Position: 66-80
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P14
Chemical type: Peptide from protein
Sequence: RSQDACIEFVQQEVD
Position: 66-80
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P14
Chemical type: Peptide from protein
Sequence: RSQDACIEFVQQEVD
Position: 66-80
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P14
Chemical type: Peptide from protein
Sequence: RSQDACIEFVQQEVD
Position: 66-80
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P14
Chemical type: Peptide from protein
Sequence: RSQDACIEFVQQEVD
Position: 66-80
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P16
Chemical type: Peptide from protein
Sequence: QQEVDYIIDHVDQHN
Position: 76-90
Source molecule (GenBank): AAF88069.1
Assay category: BCell
Result: Positive
Host: F 6-8 weeks

Epitope: P16
Chemical type: Peptide from protein
Sequence: QQEVDYIIDHVDQHN
Position: 76-90
Source molecule (GenBank): AAF88069.1
Assay category: BCell
Result: Positive
Host: F 6-8 weeks

Epitope: P16
Chemical type: Peptide from protein
Sequence: QQEVDYIIDHVDQHN
Position: 76-90
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P16
Chemical type: Peptide from protein
Sequence: QQEVDYIIDHVDQHN
Position: 76-90
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P16
Chemical type: Peptide from protein
Sequence: QQEVDYIIDHVDQHN
Position: 76-90
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P16
Chemical type: Peptide from protein
Sequence: QQEVDYIIDHVDQHN
Position: 76-90
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P17
Chemical type: Peptide from protein
Sequence: YIIDHVDQHNATEIC
Position: 81-95
Source molecule (GenBank): AAF88069.1
Assay category: BCell
Result: Positive
Host: F 6-8 weeks

Epitope: P17
Chemical type: Peptide from protein
Sequence: YIIDHVDQHNATEIC
Position: 81-95
Source molecule (GenBank): AAF88069.1
Assay category: BCell
Result: Positive
Host: F 6-8 weeks

Epitope: P17
Chemical type: Peptide from protein
Sequence: YIIDHVDQHNATEIC
Position: 81-95
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P17
Chemical type: Peptide from protein
Sequence: YIIDHVDQHNATEIC
Position: 81-95
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P17
Chemical type: Peptide from protein
Sequence: YIIDHVDQHNATEIC
Position: 81-95
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P17
Chemical type: Peptide from protein
Sequence: YIIDHVDQHNATEIC
Position: 81-95
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P17
Chemical type: Peptide from protein
Sequence: YIIDHVDQHNATEIC
Position: 81-95
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P18
Chemical type: Peptide from protein
Sequence: DQHNATEICRLIKLC
Position: 87-101
Source molecule (GenBank): AAF88069.1
Assay category: BCell
Result: Negative
Host: F 6-8 weeks

Epitope: P18
Chemical type: Peptide from protein
Sequence: DQHNATEICRLIKLC
Position: 87-101
Source molecule (GenBank): AAF88069.1
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo