Reference: Wen Tzuen Heng; Hui Xuan Lim; Kuan Onn Tan; Chit Laa Poh. "Validation of Multi-epitope Peptides Encapsulated in PLGA Nanoparticles Against Influenza A Virus." Pharmaceutical research (2023).

Epitope: M2.1
Chemical type: Peptide from protein
Sequence: SLLTEVETPIRNEWGCRCNDSSD
Position: 2-24
Source molecule (GenBank): CAA30889.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M2.1
Chemical type: Peptide from protein
Sequence: SLLTEVETPIRNEWGCRCNDSSD
Position: 2-24
Source molecule (GenBank): CAA30889.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M2.2
Chemical type: Peptide from protein
Sequence: MSLLTEVETPIRNEWGCRCNDSSD
Position: 1-24
Source molecule (GenBank): CAA30889.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M2.2
Chemical type: Peptide from protein
Sequence: MSLLTEVETPIRNEWGCRCNDSSD
Position: 1-24
Source molecule (GenBank): CAA30889.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP
Chemical type: Peptide, no natural source
Sequence: RRRILRGSVAHKRRRKTGGPIYRR
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP
Chemical type: Peptide, no natural source
Sequence: RRRILRGSVAHKRRRKTGGPIYRR
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P1
Chemical type: Peptide from protein
Sequence: ILRGSVAHK
Position: 236-244
Source molecule (GenBank): Q6PW66.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P1
Chemical type: Peptide from protein
Sequence: ILRGSVAHK
Position: 236-244
Source molecule (GenBank): Q6PW66.1
Assay category: TCell
Result: Positive-Low
Host: F 6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P1
Chemical type: Peptide from protein
Sequence: ILRGSVAHK
Position: 236-244
Source molecule (GenBank): Q6PW66.1
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P2
Chemical type: Peptide from protein
Sequence: KTGGPIYRR
Position: 62-70
Source molecule (GenBank): Q6PW66.1
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P2
Chemical type: Peptide from protein
Sequence: KTGGPIYRR
Position: 62-70
Source molecule (GenBank): Q6PW66.1
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P3
Chemical type: Peptide from protein
Sequence: SPGMMMGMFNMLSTV
Position: 404-418
Source molecule (GenBank): AWH12442.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P3
Chemical type: Peptide from protein
Sequence: SPGMMMGMFNMLSTV
Position: 404-418
Source molecule (GenBank): AWH12442.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P4
Chemical type: Peptide from protein
Sequence: MMMGMFNML
Position: 407-415
Source molecule (GenBank): AWH12442.1
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P4
Chemical type: Peptide from protein
Sequence: MMMGMFNML
Position: 407-415
Source molecule (GenBank): AWH12442.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P5
Chemical type: Peptide from protein
Sequence: DVWTYNAELLVLMEN
Position: 436-450
Source molecule (GenBank): Q6DQ33.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P5
Chemical type: Peptide from protein
Sequence: DVWTYNAELLVLMEN
Position: 436-450
Source molecule (GenBank): Q6DQ33.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P6
Chemical type: Peptide from protein
Sequence: SNVKNLYEKVRSQLKNNAK
Position: 457-475
Source molecule (GenBank): QDP16803.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P6
Chemical type: Peptide from protein
Sequence: SNVKNLYEKVRSQLKNNAK
Position: 457-475
Source molecule (GenBank): QDP16803.1
Assay category: TCell
Result: Positive
Host: F 6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P7
Chemical type: Peptide from protein
Sequence: GILGFVFTL
Position: 56-64
Source molecule (GenBank): Q0WXS5.1
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: P7
Chemical type: Peptide from protein
Sequence: GILGFVFTL
Position: 56-64
Source molecule (GenBank): Q0WXS5.1
Assay category: TCell
Result: Negative
Host: F 6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo