Reference: Felicity Hartnell; Ilaria Esposito; Leo Swadling; Anthony Brown; Chansavath Phetsouphanh; Catherine de Lara; Chiara Gentile; Bethany Turner; Lucy Dorrell; Stefania Capone; Antonella Folgori; Eleanor Barnes; Paul Klenerman. "Characterizing Hepatitis C Virus-Specific CD4<sup>+</sup> T Cells Following Viral-Vectored Vaccination, Directly Acting Antivirals, and Spontaneous Viral Cure." Hepatology (Baltimore, Md.) (2020).

Epitope: NS4b-1806-1818
Chemical type: Peptide from protein
Sequence: TLLFNILGGWVAA
Position: 1806-1818
Source molecule (GenBank): AAT00643.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: NS4b-1806-1818
Chemical type: Peptide from protein
Sequence: TLLFNILGGWVAA
Position: 1806-1818
Source molecule (GenBank): AAT00643.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: NS4b-1806-1818
Chemical type: Peptide from protein
Sequence: TLLFNILGGWVAA
Position: 1806-1818
Source molecule (GenBank): AAT00643.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: NS3-1535-1551
Chemical type: Peptide from protein
Sequence: TTVRLRAYMNTPGLPVC
Position: 1539-1555
Source molecule (GenBank): AGV23514.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: NS3-1535-1551
Chemical type: Peptide from protein
Sequence: TTVRLRAYMNTPGLPVC
Position: 1539-1555
Source molecule (GenBank): AGV23514.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: NS3-1535-1551
Chemical type: Peptide from protein
Sequence: TTVRLRAYMNTPGLPVC
Position: 1539-1555
Source molecule (GenBank): AGV23514.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: NS3-1535-1551
Chemical type: Peptide from protein
Sequence: TTVRLRAYMNTPGLPVC
Position: 1539-1555
Source molecule (GenBank): AGV23514.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: NS3-1535-1551
Chemical type: Peptide from protein
Sequence: TTVRLRAYMNTPGLPVC
Position: 1539-1555
Source molecule (GenBank): AGV23514.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: NS3-1535-1551
Chemical type: Peptide from protein
Sequence: TTVRLRAYMNTPGLPVC
Position: 1539-1555
Source molecule (GenBank): AGV23514.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: NS5b-66-81
Chemical type: Peptide from protein
Sequence: VYYLTRDPTTPLARAA
Position: 2800-2815
Source molecule (GenBank): AAT00643.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NS5b-66-81
Chemical type: Peptide from protein
Sequence: VYYLTRDPTTPLARAA
Position: 2800-2815
Source molecule (GenBank): AAT00643.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NS5b-66-81
Chemical type: Peptide from protein
Sequence: VYYLTRDPTTPLARAA
Position: 2800-2815
Source molecule (GenBank): AAT00643.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NS4b-1773-1790
Chemical type: Peptide from protein
Sequence: QYLAGLSTLPGNPAIASL
Position: 1773-1790
Source molecule (GenBank): AAT00643.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NS4b-1773-1790
Chemical type: Peptide from protein
Sequence: QYLAGLSTLPGNPAIASL
Position: 1773-1790
Source molecule (GenBank): AAT00643.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NS4b-1773-1790
Chemical type: Peptide from protein
Sequence: QYLAGLSTLPGNPAIASL
Position: 1773-1790
Source molecule (GenBank): AAT00643.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NS3-1582-1597
Chemical type: Peptide from protein
Sequence: NFPYLVAYQATVCARA
Position: 1582-1597
Source molecule (GenBank): AAT00643.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NS3-1582-1597
Chemical type: Peptide from protein
Sequence: NFPYLVAYQATVCARA
Position: 1582-1597
Source molecule (GenBank): AAT00643.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NS3-1582-1597
Chemical type: Peptide from protein
Sequence: NFPYLVAYQATVCARA
Position: 1582-1597
Source molecule (GenBank): AAT00643.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NS3-1411-1425
Chemical type: Peptide from protein
Sequence: GINAVAYYRGLDVSV
Position: 1417-1431
Source molecule (GenBank): AQW44651.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NS3-1411-1425
Chemical type: Peptide from protein
Sequence: GINAVAYYRGLDVSV
Position: 1417-1431
Source molecule (GenBank): AQW44651.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NS3-1411-1425
Chemical type: Peptide from protein
Sequence: GINAVAYYRGLDVSV
Position: 1417-1431
Source molecule (GenBank): AQW44651.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NS4b-1771-1790
Chemical type: Peptide from protein
Sequence: SGIQYLAGLSTLPGNPAIASL
Position: 1770-1790
Source molecule (GenBank): AAT00643.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NS4b-1771-1790
Chemical type: Peptide from protein
Sequence: SGIQYLAGLSTLPGNPAIASL
Position: 1770-1790
Source molecule (GenBank): AAT00643.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NS4b-1771-1790
Chemical type: Peptide from protein
Sequence: SGIQYLAGLSTLPGNPAIASL
Position: 1770-1790
Source molecule (GenBank): AAT00643.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NS2-794-810
Chemical type: Peptide from protein
Sequence: WPLLLLLLALPQRAYAQ
Position: 794-810
Source molecule (GenBank): ABR27382.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NS2-794-810
Chemical type: Peptide from protein
Sequence: WPLLLLLLALPQRAYAQ
Position: 794-810
Source molecule (GenBank): ABR27382.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NS2-794-810
Chemical type: Peptide from protein
Sequence: WPLLLLLLALPQRAYAQ
Position: 794-810
Source molecule (GenBank): ABR27382.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Direct Ex Vivo