Reference: Luigi Aurisicchio; Giuseppe Roscilli; Emanuele Marra; Laura Luberto; Rita Mancini; Nicola La Monica; Gennaro Ciliberto. "Superior Immunologic and Therapeutic Efficacy of a Xenogeneic Genetic Cancer Vaccine Targeting Carcinoembryonic Human Antigen." Human gene therapy (2015).

Epitope: hCEA-59
Chemical type: Peptide from protein
Sequence: VLYGPDAPTISPLNT
Position: 233-247
Source molecule (GenBank): P06731.4
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: hCEA-59
Chemical type: Peptide from protein
Sequence: VLYGPDAPTISPLNT
Position: 233-247
Source molecule (GenBank): P06731.4
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RhCEA-59
Chemical type: Peptide from protein
Sequence: VLYGPDAPTISPLNT
Position: 233-247
Source molecule (GenBank): AXF37597.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RhCEA-59
Chemical type: Peptide from protein
Sequence: VLYGPDAPTISPLNT
Position: 233-247
Source molecule (GenBank): AXF37597.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: hCEA-103
Chemical type: Peptide from protein
Sequence: LNVLYGPDDPTISPS
Position: 409-423
Source molecule (GenBank): P06731.4
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: hCEA-103
Chemical type: Peptide from protein
Sequence: LNVLYGPDDPTISPS
Position: 409-423
Source molecule (GenBank): P06731.4
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RhCEA-103
Chemical type: Peptide from protein
Sequence: LNVLYGPDAPTISPL
Position: 231-245
Source molecule (GenBank): AXF37597.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RhCEA-103
Chemical type: Peptide from protein
Sequence: LNVLYGPDAPTISPL
Position: 231-245
Source molecule (GenBank): AXF37597.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: hCEA-148
Chemical type: Peptide from protein
Sequence: VLYGPDTPIISPPDS
Position: 589-603
Source molecule (GenBank): P06731.4
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: hCEA-148
Chemical type: Peptide from protein
Sequence: VLYGPDTPIISPPDS
Position: 589-603
Source molecule (GenBank): P06731.4
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RhCEA-103
Chemical type: Peptide from protein
Sequence: VTYGPDTPIISPPDL
Position: 589-603
Source molecule (GenBank): AXF37597.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: RhCEA-103
Chemical type: Peptide from protein
Sequence: VTYGPDTPIISPPDL
Position: 589-603
Source molecule (GenBank): AXF37597.1
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CEA<sub>589</sub>
Chemical type: Peptide from protein
Sequence: VLYGPDTPI
Position: 589-597
Source molecule (GenBank): P06731.4
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CEA<sub>589</sub>
Chemical type: Peptide from protein
Sequence: VLYGPDTPI
Position: 589-597
Source molecule (GenBank): P06731.4
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CEA<sub>589</sub>
Chemical type: Peptide from protein
Sequence: VLYGPDTPI
Position: 589-597
Source molecule (GenBank): P06731.4
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CEA<sub>589</sub>
Chemical type: Peptide from protein
Sequence: VLYGPDTPI
Position: 589-597
Source molecule (GenBank): P06731.4
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo