Reference: Audrey Tanghe; Jean-Pierre Dangy; Gerd Pluschke; Kris Huygen. "Improved protective efficacy of a species-specific DNA vaccine encoding mycolyl-transferase Ag85A from Mycobacterium ulcerans by homologous protein boosting." PLoS neglected tropical diseases (2008).

Epitope: Ag 85 A Mu peptide (21-40)
Chemical type: Peptide from protein
Sequence: NIKVQFQSGGANSPALYLLD
Position: 64-83
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (21-40)
Chemical type: Peptide from protein
Sequence: NIKVQFQSGGANSPALYLLD
Position: 64-83
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Positive-Low
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (21-40)
Chemical type: Peptide from protein
Sequence: NIKVQFQSGGANSPALYLLD
Position: 64-83
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Positive-Low
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (21-40)
Chemical type: Peptide from protein
Sequence: NIKVQFQSGGANSPALYLLD
Position: 64-83
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (61-80)
Chemical type: Peptide from protein
Sequence: YYQSGISVAMPVGGQSSFYS
Position: 104-123
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (61-80)
Chemical type: Peptide from protein
Sequence: YYQSGISVAMPVGGQSSFYS
Position: 104-123
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (61-80)
Chemical type: Peptide from protein
Sequence: YYQSGISVAMPVGGQSSFYS
Position: 104-123
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Negative
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (61-80)
Chemical type: Peptide from protein
Sequence: YYQSGISVAMPVGGQSSFYS
Position: 104-123
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Negative
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (81-100)
Chemical type: Peptide from protein
Sequence: DWYNPACGKAGCTTYKWETF
Position: 124-143
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (81-100)
Chemical type: Peptide from protein
Sequence: DWYNPACGKAGCTTYKWETF
Position: 124-143
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (81-100)
Chemical type: Peptide from protein
Sequence: DWYNPACGKAGCTTYKWETF
Position: 124-143
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Negative
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (81-100)
Chemical type: Peptide from protein
Sequence: DWYNPACGKAGCTTYKWETF
Position: 124-143
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Negative
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (141-160)
Chemical type: Peptide from protein
Sequence: DQFVYSGSLSALLDPSQGIG
Position: 184-203
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Negative
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (141-160)
Chemical type: Peptide from protein
Sequence: DQFVYSGSLSALLDPSQGIG
Position: 184-203
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (141-160)
Chemical type: Peptide from protein
Sequence: DQFVYSGSLSALLDPSQGIG
Position: 184-203
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Negative
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (141-160)
Chemical type: Peptide from protein
Sequence: DQFVYSGSLSALLDPSQGIG
Position: 184-203
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Negative
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (241-260)
Chemical type: Peptide from protein
Sequence: QAAYNAAGGHNAVWNFDDNG
Position: 284-303
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (241-260)
Chemical type: Peptide from protein
Sequence: QAAYNAAGGHNAVWNFDDNG
Position: 284-303
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (241-260)
Chemical type: Peptide from protein
Sequence: QAAYNAAGGHNAVWNFDDNG
Position: 284-303
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (241-260)
Chemical type: Peptide from protein
Sequence: QAAYNAAGGHNAVWNFDDNG
Position: 284-303
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (261-280)
Chemical type: Peptide from protein
Sequence: THSWEYWGAQLNAMRPDLQH
Position: 304-323
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (261-280)
Chemical type: Peptide from protein
Sequence: THSWEYWGAQLNAMRPDLQH
Position: 304-323
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (261-280)
Chemical type: Peptide from protein
Sequence: THSWEYWGAQLNAMRPDLQH
Position: 304-323
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Negative
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (261-280)
Chemical type: Peptide from protein
Sequence: THSWEYWGAQLNAMRPDLQH
Position: 304-323
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Negative
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (275-294)
Chemical type: Peptide from protein
Sequence: RPDLQHTLGATPNTGDTQGA
Position: 318-337
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Negative
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (275-294)
Chemical type: Peptide from protein
Sequence: RPDLQHTLGATPNTGDTQGA
Position: 318-337
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (275-294)
Chemical type: Peptide from protein
Sequence: RPDLQHTLGATPNTGDTQGA
Position: 318-337
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mu peptide (275-294)
Chemical type: Peptide from protein
Sequence: RPDLQHTLGATPNTGDTQGA
Position: 318-337
Source molecule (GenBank): YP_908336.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (21-40)
Chemical type: Peptide from protein
Sequence: DIKVQFQSGGANSPALYLLD
Position: 64-83
Source molecule (GenBank): Q847N5.1
Assay category: TCell
Result: Negative
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (21-40)
Chemical type: Peptide from protein
Sequence: DIKVQFQSGGANSPALYLLD
Position: 64-83
Source molecule (GenBank): Q847N5.1
Assay category: TCell
Result: Positive-Low
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (21-40)
Chemical type: Peptide from protein
Sequence: DIKVQFQSGGANSPALYLLD
Position: 64-83
Source molecule (GenBank): Q847N5.1
Assay category: TCell
Result: Positive-Low
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (21-40)
Chemical type: Peptide from protein
Sequence: DIKVQFQSGGANSPALYLLD
Position: 64-83
Source molecule (GenBank): Q847N5.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (61-80)
Chemical type: Peptide from protein
Sequence: YDQSGLSVVMPVGGQSSFYS
Position: 104-123
Source molecule (GenBank): Q847N5.1
Assay category: TCell
Result: Negative
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (61-80)
Chemical type: Peptide from protein
Sequence: YDQSGLSVVMPVGGQSSFYS
Position: 104-123
Source molecule (GenBank): Q847N5.1
Assay category: TCell
Result: Negative
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (61-80)
Chemical type: Peptide from protein
Sequence: YDQSGLSVVMPVGGQSSFYS
Position: 104-123
Source molecule (GenBank): Q847N5.1
Assay category: TCell
Result: Negative
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (61-80)
Chemical type: Peptide from protein
Sequence: YDQSGLSVVMPVGGQSSFYS
Position: 104-123
Source molecule (GenBank): Q847N5.1
Assay category: TCell
Result: Positive-Low
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (81-100)
Chemical type: Peptide from protein
Sequence: DWYQPACGKAGCQTYKWETF
Position: 124-143
Source molecule (GenBank): Q847N5.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (81-100)
Chemical type: Peptide from protein
Sequence: DWYQPACGKAGCQTYKWETF
Position: 124-143
Source molecule (GenBank): Q847N5.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (81-100)
Chemical type: Peptide from protein
Sequence: DWYQPACGKAGCQTYKWETF
Position: 124-143
Source molecule (GenBank): Q847N5.1
Assay category: TCell
Result: Positive-Low
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (81-100)
Chemical type: Peptide from protein
Sequence: DWYQPACGKAGCQTYKWETF
Position: 124-143
Source molecule (GenBank): Q847N5.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (141-160)
Chemical type: Peptide from protein
Sequence: QQFVYAGAMSGLLDPSQAMG
Position: 184-203
Source molecule (GenBank): Q847N5.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (141-160)
Chemical type: Peptide from protein
Sequence: QQFVYAGAMSGLLDPSQAMG
Position: 184-203
Source molecule (GenBank): Q847N5.1
Assay category: TCell
Result: Negative
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (141-160)
Chemical type: Peptide from protein
Sequence: QQFVYAGAMSGLLDPSQAMG
Position: 184-203
Source molecule (GenBank): Q847N5.1
Assay category: TCell
Result: Negative
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (141-160)
Chemical type: Peptide from protein
Sequence: QQFVYAGAMSGLLDPSQAMG
Position: 184-203
Source molecule (GenBank): Q847N5.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (241-260): 85A
Chemical type: Peptide from protein
Sequence: QDAYNAGGGHNGVFDFPDSG
Position: 284-303
Source molecule (GenBank): Q847N5.1
Assay category: TCell
Result: Positive-Low
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (241-260): 85A
Chemical type: Peptide from protein
Sequence: QDAYNAGGGHNGVFDFPDSG
Position: 284-303
Source molecule (GenBank): Q847N5.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (241-260): 85A
Chemical type: Peptide from protein
Sequence: QDAYNAGGGHNGVFDFPDSG
Position: 284-303
Source molecule (GenBank): Q847N5.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (241-260): 85A
Chemical type: Peptide from protein
Sequence: QDAYNAGGGHNGVFDFPDSG
Position: 284-303
Source molecule (GenBank): Q847N5.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (241-260): 85B
Chemical type: Peptide from protein
Sequence: QDAYNAAGGHNAVFNFPPNG
Position: 281-300
Source molecule (GenBank): Q847N4.1
Assay category: TCell
Result: Positive-Low
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (241-260): 85B
Chemical type: Peptide from protein
Sequence: QDAYNAAGGHNAVFNFPPNG
Position: 281-300
Source molecule (GenBank): Q847N4.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (241-260): 85B
Chemical type: Peptide from protein
Sequence: QDAYNAAGGHNAVFNFPPNG
Position: 281-300
Source molecule (GenBank): Q847N4.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (241-260): 85B
Chemical type: Peptide from protein
Sequence: QDAYNAAGGHNAVFNFPPNG
Position: 281-300
Source molecule (GenBank): Q847N4.1
Assay category: TCell
Result: Positive-Low
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (261-280)
Chemical type: Peptide from protein
Sequence: THSWEYWGAQLNAMKPDLQR
Position: 304-323
Source molecule (GenBank): Q847N5.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (261-280)
Chemical type: Peptide from protein
Sequence: THSWEYWGAQLNAMKPDLQR
Position: 304-323
Source molecule (GenBank): Q847N5.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (261-280)
Chemical type: Peptide from protein
Sequence: THSWEYWGAQLNAMKPDLQR
Position: 304-323
Source molecule (GenBank): Q847N5.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (261-280)
Chemical type: Peptide from protein
Sequence: THSWEYWGAQLNAMKPDLQR
Position: 304-323
Source molecule (GenBank): Q847N5.1
Assay category: TCell
Result: Positive
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (275-295)
Chemical type: Peptide from protein
Sequence: KPDLQRALGATPNTGPAPQGA
Position: 318-338
Source molecule (GenBank): P0A4V2.1
Assay category: TCell
Result: Negative
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (275-295)
Chemical type: Peptide from protein
Sequence: KPDLQRALGATPNTGPAPQGA
Position: 318-338
Source molecule (GenBank): P0A4V2.1
Assay category: TCell
Result: Negative
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (275-295)
Chemical type: Peptide from protein
Sequence: KPDLQRALGATPNTGPAPQGA
Position: 318-338
Source molecule (GenBank): P0A4V2.1
Assay category: TCell
Result: Negative
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Ag 85 A Mtb peptide (275-295)
Chemical type: Peptide from protein
Sequence: KPDLQRALGATPNTGPAPQGA
Position: 318-338
Source molecule (GenBank): P0A4V2.1
Assay category: TCell
Result: Negative
Host: F 8-10 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo