Reference: Mohammad A Sayem; Yusuke Tomita; Akira Yuno; Masatoshi Hirayama; Atsushi Irie; Hirotake Tsukamoto; Satoru Senju; Eiji Yuba; Toshiaki Yoshikawa; Kenji Kono; Tetsuya Nakatsura; Yasuharu Nishimura. "Identification of glypican-3-derived long peptides activating both CD8<sup>+</sup> and CD4<sup>+</sup> T cells; prolonged overall survival in cancer patients with Th cell response." Oncoimmunology (2016).

Epitope: GPC3-LP1 (GPC3 92-116)
Chemical type: Peptide from protein
Sequence: LLQSASMELKFLIIQNAAVFQEAFE
Position: 92-116
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GPC3-LP1 (GPC3 92-116)
Chemical type: Peptide from protein
Sequence: LLQSASMELKFLIIQNAAVFQEAFE
Position: 92-116
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GPC3-LP1 (GPC3 92-116)
Chemical type: Peptide from protein
Sequence: LLQSASMELKFLIIQNAAVFQEAFE
Position: 92-116
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GPC3-LP1 (GPC3 92-116)
Chemical type: Peptide from protein
Sequence: LLQSASMELKFLIIQNAAVFQEAFE
Position: 92-116
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GPC3-LP1 (GPC3 92-116)
Chemical type: Peptide from protein
Sequence: LLQSASMELKFLIIQNAAVFQEAFE
Position: 92-116
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GPC3-LP1 (GPC3 92-116)
Chemical type: Peptide from protein
Sequence: LLQSASMELKFLIIQNAAVFQEAFE
Position: 92-116
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GPC3-LP1 (GPC3 92-116)
Chemical type: Peptide from protein
Sequence: LLQSASMELKFLIIQNAAVFQEAFE
Position: 92-116
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GPC3-LP2 (GPC3 137-161)
Chemical type: Peptide from protein
Sequence: LTPQAFEFVGEFFTDVSLYILGSDI
Position: 137-161
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GPC3-LP2 (GPC3 137-161)
Chemical type: Peptide from protein
Sequence: LTPQAFEFVGEFFTDVSLYILGSDI
Position: 137-161
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: GPC3-LP2 (GPC3 137-161)
Chemical type: Peptide from protein
Sequence: LTPQAFEFVGEFFTDVSLYILGSDI
Position: 137-161
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GPC3-LP2 (GPC3 137-161)
Chemical type: Peptide from protein
Sequence: LTPQAFEFVGEFFTDVSLYILGSDI
Position: 137-161
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GPC3-LP2 (GPC3 137-161)
Chemical type: Peptide from protein
Sequence: LTPQAFEFVGEFFTDVSLYILGSDI
Position: 137-161
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
MHC allele types: 143
Cell type: T cell
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GPC3-LP2 (GPC3 137-161)
Chemical type: Peptide from protein
Sequence: LTPQAFEFVGEFFTDVSLYILGSDI
Position: 137-161
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: GPC3-LP2 (GPC3 137-161)
Chemical type: Peptide from protein
Sequence: LTPQAFEFVGEFFTDVSLYILGSDI
Position: 137-161
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GPC3-LP2 (GPC3 137-161)
Chemical type: Peptide from protein
Sequence: LTPQAFEFVGEFFTDVSLYILGSDI
Position: 137-161
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GPC3-LP2 (GPC3 137-161)
Chemical type: Peptide from protein
Sequence: LTPQAFEFVGEFFTDVSLYILGSDI
Position: 137-161
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GPC3-LP2 (GPC3 137-161)
Chemical type: Peptide from protein
Sequence: LTPQAFEFVGEFFTDVSLYILGSDI
Position: 137-161
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GPC3-LP2 (GPC3 137-161)
Chemical type: Peptide from protein
Sequence: LTPQAFEFVGEFFTDVSLYILGSDI
Position: 137-161
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GPC3-LP2 (GPC3 137-161)
Chemical type: Peptide from protein
Sequence: LTPQAFEFVGEFFTDVSLYILGSDI
Position: 137-161
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GPC3-LP2 (GPC3 137-161)
Chemical type: Peptide from protein
Sequence: LTPQAFEFVGEFFTDVSLYILGSDI
Position: 137-161
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GPC3-LP2 (GPC3 137-161)
Chemical type: Peptide from protein
Sequence: LTPQAFEFVGEFFTDVSLYILGSDI
Position: 137-161
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GPC3-LP2 (GPC3 137-161)
Chemical type: Peptide from protein
Sequence: LTPQAFEFVGEFFTDVSLYILGSDI
Position: 137-161
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GPC3-LP2 (GPC3 137-161)
Chemical type: Peptide from protein
Sequence: LTPQAFEFVGEFFTDVSLYILGSDI
Position: 137-161
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GPC3-LP3 (GPC3 289-313)
Chemical type: Peptide from protein
Sequence: VVEIDKYWREYILSLEELVNGMYRI
Position: 289-313
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GPC3-LP3 (GPC3 289-313)
Chemical type: Peptide from protein
Sequence: VVEIDKYWREYILSLEELVNGMYRI
Position: 289-313
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GPC3-LP3 (GPC3 289-313)
Chemical type: Peptide from protein
Sequence: VVEIDKYWREYILSLEELVNGMYRI
Position: 289-313
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GPC3-LP3 (GPC3 289-313)
Chemical type: Peptide from protein
Sequence: VVEIDKYWREYILSLEELVNGMYRI
Position: 289-313
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GPC3-LP4 (GPC3 386-412)
Chemical type: Peptide from protein
Sequence: SRRRELIQKLKSFISFYSALPGYICSH
Position: 386-412
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GPC3-LP4 (GPC3 386-412)
Chemical type: Peptide from protein
Sequence: SRRRELIQKLKSFISFYSALPGYICSH
Position: 386-412
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GPC3-LP4 (GPC3 386-412)
Chemical type: Peptide from protein
Sequence: SRRRELIQKLKSFISFYSALPGYICSH
Position: 386-412
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GPC3-LP4 (GPC3 386-412)
Chemical type: Peptide from protein
Sequence: SRRRELIQKLKSFISFYSALPGYICSH
Position: 386-412
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GPC3-LP4 (GPC3 386-412)
Chemical type: Peptide from protein
Sequence: SRRRELIQKLKSFISFYSALPGYICSH
Position: 386-412
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GPC3-LP4 (GPC3 386-412)
Chemical type: Peptide from protein
Sequence: SRRRELIQKLKSFISFYSALPGYICSH
Position: 386-412
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: GPC3-LP4 (GPC3 386-412)
Chemical type: Peptide from protein
Sequence: SRRRELIQKLKSFISFYSALPGYICSH
Position: 386-412
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GPC3-LP4 (GPC3 386-412)
Chemical type: Peptide from protein
Sequence: SRRRELIQKLKSFISFYSALPGYICSH
Position: 386-412
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GPC3-LP4 (GPC3 386-412)
Chemical type: Peptide from protein
Sequence: SRRRELIQKLKSFISFYSALPGYICSH
Position: 386-412
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GPC3-LP5 (GPC3 556-576)
Chemical type: Peptide from protein
Sequence: GNVHSPLKLLTSMAISVVCFF
Position: 556-576
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GPC3-LP5 (GPC3 556-576)
Chemical type: Peptide from protein
Sequence: GNVHSPLKLLTSMAISVVCFF
Position: 556-576
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GPC3-LP5 (GPC3 556-576)
Chemical type: Peptide from protein
Sequence: GNVHSPLKLLTSMAISVVCFF
Position: 556-576
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GPC3-LP5 (GPC3 556-576)
Chemical type: Peptide from protein
Sequence: GNVHSPLKLLTSMAISVVCFF
Position: 556-576
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GPC3-LP5 (GPC3 556-576)
Chemical type: Peptide from protein
Sequence: GNVHSPLKLLTSMAISVVCFF
Position: 556-576
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: A2-GPC3-SP (GPC3 144-152)
Chemical type: Peptide from protein
Sequence: FVGEFFTDV
Position: 144-152
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: A2-GPC3-SP (GPC3 144-152)
Chemical type: Peptide from protein
Sequence: FVGEFFTDV
Position: 144-152
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: A2-GPC3-SP (GPC3 144-152)
Chemical type: Peptide from protein
Sequence: FVGEFFTDV
Position: 144-152
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: A2-GPC3-SP (GPC3 144-152)
Chemical type: Peptide from protein
Sequence: FVGEFFTDV
Position: 144-152
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: A2-GPC3-SP (GPC3 144-152)
Chemical type: Peptide from protein
Sequence: FVGEFFTDV
Position: 144-152
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: lymph node
Culture conditions: Direct Ex Vivo

Epitope: A2-GPC3-SP (GPC3 144-152)
Chemical type: Peptide from protein
Sequence: FVGEFFTDV
Position: 144-152
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: A2-GPC3-SP (GPC3 144-152)
Chemical type: Peptide from protein
Sequence: FVGEFFTDV
Position: 144-152
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive-Low
MHC allele types: 143
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: A2-GPC3-SP (GPC3 144-152)
Chemical type: Peptide from protein
Sequence: FVGEFFTDV
Position: 144-152
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: A2-GPC3-SP (GPC3 144-152)
Chemical type: Peptide from protein
Sequence: FVGEFFTDV
Position: 144-152
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: A2-GPC3-SP (GPC3 144-152)
Chemical type: Peptide from protein
Sequence: FVGEFFTDV
Position: 144-152
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: A24-GPC3-SP (GPC3 298-306)
Chemical type: Peptide from protein
Sequence: EYILSLEEL
Position: 298-306
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: A24-GPC3-SP (GPC3 298-306)
Chemical type: Peptide from protein
Sequence: EYILSLEEL
Position: 298-306
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: A24-GPC3-SP (GPC3 298-306)
Chemical type: Peptide from protein
Sequence: EYILSLEEL
Position: 298-306
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: A24-GPC3-SP (GPC3 298-306)
Chemical type: Peptide from protein
Sequence: EYILSLEEL
Position: 298-306
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: A24-GPC3-SP (GPC3 298-306)
Chemical type: Peptide from protein
Sequence: EYILSLEEL
Position: 298-306
Source molecule (GenBank): P51654.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated