Reference: Ting Wu; Jing Guan; Andreas Handel; David C Tscharke; John Sidney; Alessandro Sette; Linda M Wakim; Xavier Y X Sng; Paul G Thomas; Nathan P Croft; Anthony W Purcell; Nicole L La Gruta. "Quantification of epitope abundance reveals the effect of direct and cross-presentation on influenza CTL responses." Nature communications (2019).

Epitope: NP 36–43
Chemical type: Peptide from protein
Sequence: IGRFYIQM
Position: 36-43
Source molecule (GenBank): AAA43467.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 36–43
Chemical type: Peptide from protein
Sequence: IGRFYIQM
Position: 36-43
Source molecule (GenBank): AAA43467.1
Assay category: MhcLigandElution
Result: Positive

Epitope: NP 36–43
Chemical type: Peptide from protein
Sequence: IGRFYIQM
Position: 36-43
Source molecule (GenBank): AAA43467.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 3303.0

Epitope: NP 36–43
Chemical type: Peptide from protein
Sequence: IGRFYIQM
Position: 36-43
Source molecule (GenBank): AAA43467.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 1.9

Epitope: NP 55–63
Chemical type: Peptide from protein
Sequence: RLIQNSLTI
Position: 55-63
Source molecule (GenBank): AAA43467.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 55–63
Chemical type: Peptide from protein
Sequence: RLIQNSLTI
Position: 55-63
Source molecule (GenBank): AAA43467.1
Assay category: MhcLigandElution
Result: Positive

Epitope: NP 55–63
Chemical type: Peptide from protein
Sequence: RLIQNSLTI
Position: 55-63
Source molecule (GenBank): AAA43467.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 184.0

Epitope: NP 55–63
Chemical type: Peptide from protein
Sequence: RLIQNSLTI
Position: 55-63
Source molecule (GenBank): AAA43467.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 463.0

Epitope: NP 366–374
Chemical type: Peptide from protein
Sequence: ASNENMETM
Position: 366-374
Source molecule (GenBank): AAA43467.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 366–374
Chemical type: Peptide from protein
Sequence: ASNENMETM
Position: 366-374
Source molecule (GenBank): AAA43467.1
Assay category: MhcLigandElution
Result: Positive

Epitope: NP 366–374
Chemical type: Peptide from protein
Sequence: ASNENMETM
Position: 366-374
Source molecule (GenBank): AAA43467.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 18.0

Epitope: NP 366–374
Chemical type: Peptide from protein
Sequence: ASNENMETM
Position: 366-374
Source molecule (GenBank): AAA43467.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 40000.0

Epitope: PA 224–233
Chemical type: Peptide from protein
Sequence: SSLENFRAYV
Position: 224-233
Source molecule (GenBank): P03433.2
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PA 224–233
Chemical type: Peptide from protein
Sequence: SSLENFRAYV
Position: 224-233
Source molecule (GenBank): P03433.2
Assay category: MhcLigandElution
Result: Positive

Epitope: PA 224–233
Chemical type: Peptide from protein
Sequence: SSLENFRAYV
Position: 224-233
Source molecule (GenBank): P03433.2
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 0.16

Epitope: PA 224–233
Chemical type: Peptide from protein
Sequence: SSLENFRAYV
Position: 224-233
Source molecule (GenBank): P03433.2
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 38.0

Epitope: PB1 653–660
Chemical type: Peptide from protein
Sequence: KNMEYDAV
Position: 653-660
Source molecule (GenBank): P03431.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PB1 653–660
Chemical type: Peptide from protein
Sequence: KNMEYDAV
Position: 653-660
Source molecule (GenBank): P03431.1
Assay category: MhcLigandElution
Result: Positive

Epitope: PB1 653–660
Chemical type: Peptide from protein
Sequence: KNMEYDAV
Position: 653-660
Source molecule (GenBank): P03431.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 40000.0

Epitope: PB1 653–660
Chemical type: Peptide from protein
Sequence: KNMEYDAV
Position: 653-660
Source molecule (GenBank): P03431.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 156.0

Epitope: PB1 703–711
Chemical type: Peptide from protein
Sequence: SSYRRPVGI
Position: 703-711
Source molecule (GenBank): AAA43637.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PB1 703–711
Chemical type: Peptide from protein
Sequence: SSYRRPVGI
Position: 703-711
Source molecule (GenBank): AAA43637.1
Assay category: MhcLigandElution
Result: Positive

Epitope: PB1 703–711
Chemical type: Peptide from protein
Sequence: SSYRRPVGI
Position: 703-711
Source molecule (GenBank): AAA43637.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 28988.0

Epitope: PB1 703–711
Chemical type: Peptide from protein
Sequence: SSYRRPVGI
Position: 703-711
Source molecule (GenBank): AAA43637.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 1.9

Epitope: PB1-F2 62–70
Chemical type: Peptide from protein
Sequence: LSLRNPILV
Position: 62-70
Source molecule (GenBank): P0C0U1.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: PB1-F2 62–70
Chemical type: Peptide from protein
Sequence: LSLRNPILV
Position: 62-70
Source molecule (GenBank): P0C0U1.1
Assay category: MhcLigandElution
Result: Positive

Epitope: PB1-F2 62–70
Chemical type: Peptide from protein
Sequence: LSLRNPILV
Position: 62-70
Source molecule (GenBank): P0C0U1.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 18.0

Epitope: PB1-F2 62–70
Chemical type: Peptide from protein
Sequence: LSLRNPILV
Position: 62-70
Source molecule (GenBank): P0C0U1.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 12403.0

Epitope: NS2 8–16
Chemical type: Peptide from protein
Sequence: SFQDILLRM
Position: 8-16
Source molecule (GenBank): NP_040983.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS2 8–16
Chemical type: Peptide from protein
Sequence: SFQDILLRM
Position: 8-16
Source molecule (GenBank): NP_040983.1
Assay category: MhcLigandElution
Result: Positive

Epitope: NS2 8–16
Chemical type: Peptide from protein
Sequence: SFQDILLRM
Position: 8-16
Source molecule (GenBank): NP_040983.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 10687.0

Epitope: NS2 8–16
Chemical type: Peptide from protein
Sequence: SFQDILLRM
Position: 8-16
Source molecule (GenBank): NP_040983.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 185.0

Epitope: M1 128–135
Chemical type: Peptide from protein
Sequence: MGLIYNRM
Position: 128-135
Source molecule (GenBank): 1384890435
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M1 128–135
Chemical type: Peptide from protein
Sequence: MGLIYNRM
Position: 128-135
Source molecule (GenBank): 1384890435
Assay category: MhcLigandElution
Result: Positive

Epitope: M1 128–135
Chemical type: Peptide from protein
Sequence: MGLIYNRM
Position: 128-135
Source molecule (GenBank): 1384890435
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 40000.0

Epitope: M1 128–135
Chemical type: Peptide from protein
Sequence: MGLIYNRM
Position: 128-135
Source molecule (GenBank): 1384890435
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 92.6

Epitope: M1 207–216
Chemical type: Peptide from protein
Sequence: SQARQMVQAM
Position: 207-216
Source molecule (GenBank): AAM75161.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M1 207–216
Chemical type: Peptide from protein
Sequence: SQARQMVQAM
Position: 207-216
Source molecule (GenBank): AAM75161.1
Assay category: MhcLigandElution
Result: Positive

Epitope: M1 207–216
Chemical type: Peptide from protein
Sequence: SQARQMVQAM
Position: 207-216
Source molecule (GenBank): AAM75161.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 11225.0

Epitope: M1 207–216
Chemical type: Peptide from protein
Sequence: SQARQMVQAM
Position: 207-216
Source molecule (GenBank): AAM75161.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 26943.0

Epitope: M1 227–237
Chemical type: Peptide from protein
Sequence: AGLKNDLLENL
Position: 227-237
Source molecule (GenBank): P03485.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M1 227–237
Chemical type: Peptide from protein
Sequence: AGLKNDLLENL
Position: 227-237
Source molecule (GenBank): P03485.1
Assay category: MhcLigandElution
Result: Positive

Epitope: M1 227–237
Chemical type: Peptide from protein
Sequence: AGLKNDLLENL
Position: 227-237
Source molecule (GenBank): P03485.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 5285.0

Epitope: M1 227–237
Chemical type: Peptide from protein
Sequence: AGLKNDLLENL
Position: 227-237
Source molecule (GenBank): P03485.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 3306.0

Epitope: HA 304–311
Chemical type: Peptide from protein
Sequence: SSLPYQNI
Position: 304-311
Source molecule (GenBank): Q8JUU5
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 304–311
Chemical type: Peptide from protein
Sequence: SSLPYQNI
Position: 304-311
Source molecule (GenBank): Q8JUU5
Assay category: MhcLigandElution
Result: Positive

Epitope: HA 304–311
Chemical type: Peptide from protein
Sequence: SSLPYQNI
Position: 304-311
Source molecule (GenBank): Q8JUU5
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 40000.0

Epitope: HA 304–311
Chemical type: Peptide from protein
Sequence: SSLPYQNI
Position: 304-311
Source molecule (GenBank): Q8JUU5
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 4.7

Epitope: HA 308–316
Chemical type: Peptide from protein
Sequence: YQNIHPVTI
Position: 308-316
Source molecule (GenBank): Q8JUU5
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 308–316
Chemical type: Peptide from protein
Sequence: YQNIHPVTI
Position: 308-316
Source molecule (GenBank): Q8JUU5
Assay category: MhcLigandElution
Result: Positive

Epitope: HA 308–316
Chemical type: Peptide from protein
Sequence: YQNIHPVTI
Position: 308-316
Source molecule (GenBank): Q8JUU5
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 1971.0

Epitope: HA 308–316
Chemical type: Peptide from protein
Sequence: YQNIHPVTI
Position: 308-316
Source molecule (GenBank): Q8JUU5
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 1138.0

Epitope: HA 389–399
Chemical type: Peptide from protein
Sequence: NGITNKVNTVI
Position: 389-399
Source molecule (GenBank): Q8JUU5
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 389–399
Chemical type: Peptide from protein
Sequence: NGITNKVNTVI
Position: 389-399
Source molecule (GenBank): Q8JUU5
Assay category: MhcLigandElution
Result: Positive

Epitope: HA 389–399
Chemical type: Peptide from protein
Sequence: NGITNKVNTVI
Position: 389-399
Source molecule (GenBank): Q8JUU5
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 118.0

Epitope: HA 389–399
Chemical type: Peptide from protein
Sequence: NGITNKVNTVI
Position: 389-399
Source molecule (GenBank): Q8JUU5
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 22004.0

Epitope: NA 181–191
Chemical type: Peptide from protein
Sequence: SGPDNGAVAVL
Position: 181-191
Source molecule (GenBank): 0704313A
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NA 181–191
Chemical type: Peptide from protein
Sequence: SGPDNGAVAVL
Position: 181-191
Source molecule (GenBank): 0704313A
Assay category: MhcLigandElution
Result: Positive

Epitope: NA 181–191
Chemical type: Peptide from protein
Sequence: SGPDNGAVAVL
Position: 181-191
Source molecule (GenBank): 0704313A
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 585.0

Epitope: NA 181–191
Chemical type: Peptide from protein
Sequence: SGPDNGAVAVL
Position: 181-191
Source molecule (GenBank): 0704313A
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 64.0

Epitope: HA 467-476
Chemical type: Peptide from protein
Sequence: SQLKNNAKEI
Position: 467-476
Source molecule (GenBank): AAA79714.1
Assay category: MhcLigandElution
Result: Positive

Epitope: HA 467-476
Chemical type: Peptide from protein
Sequence: SQLKNNAKEI
Position: 467-476
Source molecule (GenBank): AAA79714.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 173.0

Epitope: HA 467-476
Chemical type: Peptide from protein
Sequence: SQLKNNAKEI
Position: 467-476
Source molecule (GenBank): AAA79714.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 40000.0