Reference: Johannes Gebert; Ozkan Gelincik; Mine Oezcan-Wahlbrink; Jason D Marshall; Alejandro Hernandez-Sanchez; Katharina Urban; Mark Long; Eduardo Cortes; Elena Tosti; Eva-Maria Katzenmaier; Yurong Song; Ali Elsaadi; Nan Deng; Eduardo Vilar; Vera Fuchs; Nina Nelius; Yan P Yuan; Aysel Ahadova; Shizuko Sei; Robert H Shoemaker; Asad Umar; Lei Wei; Song Liu; Peer Bork; Winfried Edelmann; Magnus von Knebel Doeberitz; Steven M Lipkin; Matthias Kloor. "Recurrent Frameshift Neoantigen Vaccine Elicits Protective Immunity With Reduced Tumor Burden and Improved Overall Survival in a Lynch Syndrome Mouse Model." Gastroenterology (2021).

Epitope: Nacad wt
Chemical type: Peptide from protein
Sequence: VIYAPPPPSRGAVAVLATPS
Position: 30-49
Source molecule (GenBank): Q5SWP3.1
Assay category: BCell
Result: Positive-Low

Epitope: Nacad wt
Chemical type: Peptide from protein
Sequence: VIYAPPPPSRGAVAVLATPS
Position: 30-49
Source molecule (GenBank): Q5SWP3.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Nacad (-1) N
Chemical type: Peptide from protein
Sequence: VIYAPPPPAEGRW
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Nacad (-1) C
Chemical type: Peptide from protein
Sequence: AEGRWPCWLLRAH
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Xirp1 wt
Chemical type: Peptide from protein
Sequence: GKGPGGPPPELPKKGDVQTI
Position: 579-598
Source molecule (GenBank): XP_030100108.2
Assay category: BCell
Result: Positive-Low

Epitope: Xirp1 wt
Chemical type: Peptide from protein
Sequence: GKGPGGPPPELPKKGDVQTI
Position: 579-598
Source molecule (GenBank): XP_030100108.2
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Xirp1 (-1) N
Chemical type: Peptide from protein
Sequence: GKGPGGPPLSSPKRVMYRLS
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Xirp1 (-1) C
Chemical type: Peptide from protein
Sequence: VMYRLSVGCLRPTL
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Maz wt
Chemical type: Peptide from protein
Sequence: PCTLLAPPFPVLGLDSRGVG
Position: 6-25
Source molecule (GenBank): P56671.1
Assay category: BCell
Result: Positive-Low

Epitope: Maz wt
Chemical type: Peptide from protein
Sequence: PCTLLAPPFPVLGLDSRGVG
Position: 6-25
Source molecule (GenBank): P56671.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Maz (-1) N
Chemical type: Peptide from protein
Sequence: PCTLLAPPSPCWAW
Assay category: TCell
Result: Positive-Low
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Maz (-1) C
Chemical type: Peptide from protein
Sequence: SPCWAWTPGGWAAS
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Senp6 wt
Chemical type: Peptide from protein
Sequence: VKCSMKKKNHAINENEEPSN
Position: 876-895
Source molecule (GenBank): Q6P7W0.3
Assay category: BCell
Result: Positive-Low

Epitope: Senp6 wt
Chemical type: Peptide from protein
Sequence: VKCSMKKKNHAINENEEPSN
Position: 876-895
Source molecule (GenBank): Q6P7W0.3
Assay category: TCell
Result: Positive-Low
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Senp6 (-1) N
Chemical type: Peptide from protein
Sequence: VKCSMKKKIMLSMKMKNQVTENLRA
Assay category: TCell
Result: Positive-Low
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Senp6 (-1) C
Chemical type: Peptide from protein
Sequence: LRAARTFVIEPKVRMASGMNASVLYIIQMP
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Nacad FS(-2)
Chemical type: Peptide from protein
Sequence: DVIYAPPPQQRGGGRAGYSERIDGQRDRETGVSAGTRPGHARGGCGR
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Maz FS(-2)
Chemical type: Peptide from protein
Sequence: FPCTLLAPLPRAGPGLPGGGRPHELLPATSGSRPEPPAGRG
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Rif1 FS(-2)
Chemical type: Peptide from protein
Sequence: AHTKDKKKSETVGQTETRIFISKNKEW
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Xirp1 FS(-1)
Chemical type: Peptide from protein
Sequence: GKGPGGPPLSSPKRVMYRLSVGCLRPTL
Assay category: BCell
Result: Positive-Low

Epitope: Xirp1 FS(-1)
Chemical type: Peptide from protein
Sequence: GKGPGGPPLSSPKRVMYRLSVGCLRPTL
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Xirp1 FS(-1)
Chemical type: Peptide from protein
Sequence: GKGPGGPPLSSPKRVMYRLSVGCLRPTL
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Senp6 FS(-1)
Chemical type: Peptide from protein
Sequence: VKCSMKKKIMLSMKMKNQVTENLRARTFVIEPKVRMASGMNASVLYIIQM
Assay category: BCell
Result: Positive-High

Epitope: Senp6 FS(-1)
Chemical type: Peptide from protein
Sequence: VKCSMKKKIMLSMKMKNQVTENLRARTFVIEPKVRMASGMNASVLYIIQM
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Senp6 FS(-1)
Chemical type: Peptide from protein
Sequence: VKCSMKKKIMLSMKMKNQVTENLRARTFVIEPKVRMASGMNASVLYIIQM
Assay category: TCell
Result: Positive
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Marcks FS(-1)
Chemical type: Peptide from protein
Sequence: SSETPKKKRSAFPSRSPSS
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Maz FS(-1)
Chemical type: Peptide from protein
Sequence: PCTLLAPPSPCWAWTPGGWAAS
Assay category: BCell
Result: Positive-High

Epitope: Maz FS(-1)
Chemical type: Peptide from protein
Sequence: PCTLLAPPSPCWAWTPGGWAAS
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Tmem107 FS(-1)
Chemical type: Peptide from protein
Sequence: TQYFGMGGVVENRSQI
Position: 141-156
Source molecule (GenBank): EDL10476.1
Assay category: TCell
Result: Negative
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Nacad FS(-1)
Chemical type: Peptide from protein
Sequence: VIYAPPPPAEGRWPCWLLRAH
Assay category: BCell
Result: Positive

Epitope: Nacad FS(-1)
Chemical type: Peptide from protein
Sequence: VIYAPPPPAEGRWPCWLLRAH
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Nacad FS(-1)
Chemical type: Peptide from protein
Sequence: VIYAPPPPAEGRWPCWLLRAH
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo