Reference: E A James; S D van Haren; R A Ettinger; K Fijnvandraat; J A Liberman; W W Kwok; J Voorberg; K P Pratt. "T-cell responses in two unrelated hemophilia A inhibitor subjects include an epitope at the factor VIII R593C missense site." Journal of thrombosis and haemostasis : JTH (2011).

Epitope: FVIII-A2 429-448
Chemical type: Peptide from protein
Sequence: MAYTDETFKTREAIQHESGI
Position: 429-448
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII-A2 429-448
Chemical type: Peptide from protein
Sequence: MAYTDETFKTREAIQHESGI
Position: 429-448
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 8900.0

Epitope: FVIII-A2 453-472
Chemical type: Peptide from protein
Sequence: LYGEVGDTLLIIFKNQASRP
Position: 453-472
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII-A2 453-472
Chemical type: Peptide from protein
Sequence: LYGEVGDTLLIIFKNQASRP
Position: 453-472
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 200.0

Epitope: FVIII-A2 469-488
Chemical type: Peptide from protein
Sequence: ASRPYNIYPHGITDVRPLYS
Position: 469-488
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII-A2 469-488
Chemical type: Peptide from protein
Sequence: ASRPYNIYPHGITDVRPLYS
Position: 469-488
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 100000.0

Epitope: FVIII-A2 501-520
Chemical type: Peptide from protein
Sequence: FPILPGEIFKYKWTVTVEDG
Position: 501-520
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII-A2 501-520
Chemical type: Peptide from protein
Sequence: FPILPGEIFKYKWTVTVEDG
Position: 501-520
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 100000.0

Epitope: FVIII-A2 541-560
Chemical type: Peptide from protein
Sequence: RDLASGLIGPLLICYKESVD
Position: 541-560
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII-A2 541-560
Chemical type: Peptide from protein
Sequence: RDLASGLIGPLLICYKESVD
Position: 541-560
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 25000.0

Epitope: FVIII-A2 581-600
Chemical type: Peptide from protein
Sequence: ENRSWYLTENIQRFLPNPAG
Position: 581-600
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII-A2 581-600
Chemical type: Peptide from protein
Sequence: ENRSWYLTENIQRFLPNPAG
Position: 581-600
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 500.0

Epitope: FVIII-A2 581-600
Chemical type: Peptide from protein
Sequence: ENRSWYLTENIQRFLPNPAG
Position: 581-600
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 38000.0

Epitope: FVIII-A2 581-600
Chemical type: Peptide from protein
Sequence: ENRSWYLTENIQRFLPNPAG
Position: 581-600
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 44000.0

Epitope: FVIII-A2 581-600
Chemical type: Peptide from protein
Sequence: ENRSWYLTENIQRFLPNPAG
Position: 581-600
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 48000.0

Epitope: FVIII-A2 581-600
Chemical type: Peptide from protein
Sequence: ENRSWYLTENIQRFLPNPAG
Position: 581-600
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 9800.0

Epitope: FVIII-A2 581-600
Chemical type: Peptide from protein
Sequence: ENRSWYLTENIQRFLPNPAG
Position: 581-600
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 3700.0

Epitope: FVIII-A2 589-608
Chemical type: Peptide from protein
Sequence: ENIQRFLPNPAGVQLEDPEF
Position: 589-608
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII-A2 589-608
Chemical type: Peptide from protein
Sequence: ENIQRFLPNPAGVQLEDPEF
Position: 589-608
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: FVIII-A2 589-608
Chemical type: Peptide from protein
Sequence: ENIQRFLPNPAGVQLEDPEF
Position: 589-608
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: FVIII-A2 589-608
Chemical type: Peptide from protein
Sequence: ENIQRFLPNPAGVQLEDPEF
Position: 589-608
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: FVIII-A2 589-608
Chemical type: Peptide from protein
Sequence: ENIQRFLPNPAGVQLEDPEF
Position: 589-608
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: FVIII-A2 589-608
Chemical type: Peptide from protein
Sequence: ENIQRFLPNPAGVQLEDPEF
Position: 589-608
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: FVIII-A2 589-608
Chemical type: Peptide from protein
Sequence: ENIQRFLPNPAGVQLEDPEF
Position: 589-608
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: FVIII-A2 589-608
Chemical type: Peptide from protein
Sequence: ENIQRFLPNPAGVQLEDPEF
Position: 589-608
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 38000.0

Epitope: FVIII-A2 589-608
Chemical type: Peptide from protein
Sequence: ENIQRFLPNPAGVQLEDPEF
Position: 589-608
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 59000.0

Epitope: FVIII-A2 589-608
Chemical type: Peptide from protein
Sequence: ENIQRFLPNPAGVQLEDPEF
Position: 589-608
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 4600.0

Epitope: FVIII-A2 589-608
Chemical type: Peptide from protein
Sequence: ENIQRFLPNPAGVQLEDPEF
Position: 589-608
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 500.0

Epitope: FVIII-A2 589-608
Chemical type: Peptide from protein
Sequence: ENIQRFLPNPAGVQLEDPEF
Position: 589-608
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 4200.0

Epitope: FVIII-A2 589-608
Chemical type: Peptide from protein
Sequence: ENIQRFLPNPAGVQLEDPEF
Position: 589-608
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 50000.0

Epitope: FVIII-A2 605-624
Chemical type: Peptide from protein
Sequence: DPEFQASNIMHSINGYVFDS
Position: 605-624
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII-A2 605-624
Chemical type: Peptide from protein
Sequence: DPEFQASNIMHSINGYVFDS
Position: 605-624
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 8900.0

Epitope: FVIII-A2 637-656
Chemical type: Peptide from protein
Sequence: WYILSIGAQTDFLSVFFSGY
Position: 637-656
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII-A2 637-656
Chemical type: Peptide from protein
Sequence: WYILSIGAQTDFLSVFFSGY
Position: 637-656
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 300.0

Epitope: FVIII-A2 653-672
Chemical type: Peptide from protein
Sequence: FSGYTFKHKMVYEDTLTLFP
Position: 653-672
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 20000.0

Epitope: FVIII-A2 661-680
Chemical type: Peptide from protein
Sequence: KMVYEDTLTLFPFSGETVFM
Position: 661-680
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII-A2 661-680
Chemical type: Peptide from protein
Sequence: KMVYEDTLTLFPFSGETVFM
Position: 661-680
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 100000.0

Epitope: FVIII-A2 677-696
Chemical type: Peptide from protein
Sequence: TVFMSMENPGLWILGCHNSD
Position: 677-696
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 100000.0

Epitope: FVIII-A2 685-704
Chemical type: Peptide from protein
Sequence: PGLWILGCHNSDFRNRGMTA
Position: 685-704
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII-A2 685-704
Chemical type: Peptide from protein
Sequence: PGLWILGCHNSDFRNRGMTA
Position: 685-704
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 100000.0

Epitope: FVIII-A2 373-392
Chemical type: Peptide from protein
Sequence: SVAKKHPKTWVHYIAAEEED
Position: 373-392
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII-A2 381-400
Chemical type: Peptide from protein
Sequence: TWVHYIAAEEEDWDYAPLVL
Position: 381-400
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII-A2 389-408
Chemical type: Peptide from protein
Sequence: EEEDWDYAPLVLAPDDRSYK
Position: 389-408
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII-A2 397-416
Chemical type: Peptide from protein
Sequence: PLVLAPDDRSYKSQYLNNGP
Position: 397-416
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII-A2 405-424
Chemical type: Peptide from protein
Sequence: RSYKSQYLNNGPQRIGRKYK
Position: 405-424
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII-A2 413-432
Chemical type: Peptide from protein
Sequence: NNGPQRIGRKYKKVRFMAYT
Position: 413-432
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII-A2 421-440
Chemical type: Peptide from protein
Sequence: RKYKKVRFMAYTDETFKTRE
Position: 421-440
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII-A2 437-456
Chemical type: Peptide from protein
Sequence: KTREAIQHESGILGPLLYGE
Position: 437-456
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII-A2 565-584
Chemical type: Peptide from protein
Sequence: QIMSDKRNVILFSVFDENRS
Position: 565-584
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII-A2 573-592
Chemical type: Peptide from protein
Sequence: VILFSVFDENRSWYLTENIQ
Position: 573-592
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII-A2 597-616
Chemical type: Peptide from protein
Sequence: NPAGVQLEDPEFQASNIMHS
Position: 597-616
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII-C2 2170-2189
Chemical type: Peptide from protein
Sequence: DLNSCSMPLGMESKAISDAQ
Position: 2189-2208
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII-C2 2178-2197
Chemical type: Peptide from protein
Sequence: LGMESKAISDAQITASSYFT
Position: 2197-2216
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII-C2 2187-2205
Chemical type: Peptide from protein
Sequence: DAQITASSYFTNMFATWSP
Position: 2206-2224
Source molecule (GenBank): AAA52420.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FVIII 592-603
Chemical type: Peptide from protein
Sequence: QRFLPNPAGVQL
Position: 592-603
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: FVIII 592-603
Chemical type: Peptide from protein
Sequence: QRFLPNPAGVQL
Position: 592-603
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: FVIII 592-603
Chemical type: Peptide from protein
Sequence: QRFLPNPAGVQL
Position: 592-603
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: FVIII 592-603
Chemical type: Peptide from protein
Sequence: QRFLPNPAGVQL
Position: 592-603
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: FVIII 592-603
Chemical type: Peptide from protein
Sequence: QRFLPNPAGVQL
Position: 592-603
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: FVIII 592-603
Chemical type: Peptide from protein
Sequence: QRFLPNPAGVQL
Position: 592-603
Source molecule (GenBank): 3CDZ_A
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: FVIII 592-603
Chemical type: Peptide from protein
Sequence: QRFLPNPAGVQL
Position: 592-603
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 500.0

Epitope: FVIII 593-603
Chemical type: Peptide from protein
Sequence: RFLPNPAGVQL
Position: 593-603
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 5700.0

Epitope: FVIII 594-603
Chemical type: Peptide from protein
Sequence: FLPNPAGVQL
Position: 594-603
Source molecule (GenBank): 3CDZ_A
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 12800.0