Reference: Chiara M Cattaneo; Thomas Battaglia; Jos Urbanus; Ziva Moravec; Rhianne Voogd; Rosa de Groot; Koen J Hartemink; John B A G Haanen; Emile E Voest; Ton N Schumacher; Wouter Scheper. "Identification of patient-specific CD4<sup>+</sup> and CD8<sup>+</sup> T cell neoantigens through HLA-unbiased genetic screens." Nature biotechnology (2023).

Epitope: EPHB6-WT
Chemical type: Peptide from protein
Sequence: RAHVPYILEVQAVNGVSELSPDPPQAAAINV
Position: 451-481
Source molecule (GenBank): O15197.4
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD4+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: NEURL1-WT
Chemical type: Peptide from protein
Sequence: HSRALPAQLDGDLRFHALRAGAHVRILDEQT
Position: 282-312
Source molecule (GenBank): O76050.1
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD8+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: NEURL1-WT
Chemical type: Peptide from protein
Sequence: HSRALPAQLDGDLRFHALRAGAHVRILDEQT
Position: 282-312
Source molecule (GenBank): O76050.1
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD4+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: SHOC2-WT
Chemical type: Peptide from protein
Sequence: STLPESLLSSLVKLNSLTLARNCFQLYPVGG
Position: 320-350
Source molecule (GenBank): Q9UQ13.2
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD4+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: SHOC2-WT
Chemical type: Peptide from protein
Sequence: STLPESLLSSLVKLNSLTLARNCFQLYPVGG
Position: 320-350
Source molecule (GenBank): Q9UQ13.2
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD8+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: CCDC91-WT
Chemical type: Peptide from protein
Sequence: SAIEKQAHKCEELLNAQSALFSILPAIGCF
Position: 238-267
Source molecule (GenBank): F5GWB1.1
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD8+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: CCDC91-WT
Chemical type: Peptide from protein
Sequence: SAIEKQAHKCEELLNAQSALFSILPAIGCF
Position: 238-267
Source molecule (GenBank): F5GWB1.1
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD4+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: CELA1-WT
Chemical type: Peptide from protein
Sequence: ETNARVVGGTEAGRNSWPSQISLQYRSGGSR
Position: 14-44
Source molecule (GenBank): Q9UNI1.2
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD4+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: CELA1-WT
Chemical type: Peptide from protein
Sequence: ETNARVVGGTEAGRNSWPSQISLQYRSGGSR
Position: 14-44
Source molecule (GenBank): Q9UNI1.2
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD8+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: SP1-WT
Chemical type: Peptide from protein
Sequence: CGKVYGKTSHLRAHLRWHTGERPFMCTWSYC
Position: 633-663
Source molecule (GenBank): P08047.3
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD8+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: SP1-WT
Chemical type: Peptide from protein
Sequence: CGKVYGKTSHLRAHLRWHTGERPFMCTWSYC
Position: 633-663
Source molecule (GenBank): P08047.3
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD4+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: IGF1-WT
Chemical type: Peptide from protein
Sequence: DMPKTQKYQPPSTNKNTKSQRRKGSTFEERK
Position: 128-158
Source molecule (GenBank): P05019-4
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD8+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: IGF1-WT
Chemical type: Peptide from protein
Sequence: DMPKTQKYQPPSTNKNTKSQRRKGSTFEERK
Position: 128-158
Source molecule (GenBank): P05019-4
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD4+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: ISLR2-WT
Chemical type: Peptide from protein
Sequence: HAPGAGGEPDGQAPTSERKSTAKGRGNSVLP
Position: 380-410
Source molecule (GenBank): Q6UXK2.1
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD4+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: ISLR2-WT
Chemical type: Peptide from protein
Sequence: HAPGAGGEPDGQAPTSERKSTAKGRGNSVLP
Position: 380-410
Source molecule (GenBank): Q6UXK2.1
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD8+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: PRSS54-WT
Chemical type: Peptide from protein
Sequence: KWITSKAERAGPPLSSLHHWEKLISFSHHGP
Position: 262-292
Source molecule (GenBank): Q6PEW0.3
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD8+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: PRSS54-WT
Chemical type: Peptide from protein
Sequence: KWITSKAERAGPPLSSLHHWEKLISFSHHGP
Position: 262-292
Source molecule (GenBank): Q6PEW0.3
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD4+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: ATP6V0D1-WT
Chemical type: Peptide from protein
Sequence: LKEQECRNIVWIAECIAQRHRAKIDNYIPIF
Position: 321-351
Source molecule (GenBank): P61421.1
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD8+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: ATP6V0D1-WT
Chemical type: Peptide from protein
Sequence: LKEQECRNIVWIAECIAQRHRAKIDNYIPIF
Position: 321-351
Source molecule (GenBank): P61421.1
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD4+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: MSI2-WT
Chemical type: Peptide from protein
Sequence: FDKTTNRHRGFGFVTFENEDVVEKVCEIHFH
Position: 143-173
Source molecule (GenBank): Q96DH6.1
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD8+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: MSI2-WT
Chemical type: Peptide from protein
Sequence: FDKTTNRHRGFGFVTFENEDVVEKVCEIHFH
Position: 143-173
Source molecule (GenBank): Q96DH6.1
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD4+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: AANAT-WT
Chemical type: Peptide from protein
Sequence: FYERFSFHAVGPCAITVGSLTFMELHCSLRG
Position: 167-197
Source molecule (GenBank): Q16613.1
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD8+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: AANAT-WT
Chemical type: Peptide from protein
Sequence: FYERFSFHAVGPCAITVGSLTFMELHCSLRG
Position: 167-197
Source molecule (GenBank): Q16613.1
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD4+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: MAP4K1-WT
Chemical type: Peptide from protein
Sequence: SGTISPHCNLLLPGSSNSPASASRVAGITGL
Position: 803-833
Source molecule (GenBank): Q92918.1
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD4+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: MAP4K1-WT
Chemical type: Peptide from protein
Sequence: SGTISPHCNLLLPGSSNSPASASRVAGITGL
Position: 803-833
Source molecule (GenBank): Q92918.1
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD8+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: ZNF83-WT
Chemical type: Peptide from protein
Sequence: HLAQHQRIHTGEKPYKCNECGKVFSHKSSLV
Position: 275-305
Source molecule (GenBank): P51522.3
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD8+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: ZNF83-WT
Chemical type: Peptide from protein
Sequence: HLAQHQRIHTGEKPYKCNECGKVFSHKSSLV
Position: 275-305
Source molecule (GenBank): P51522.3
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD4+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: CFAP47-WT
Chemical type: Peptide from protein
Sequence: PVTLELSSNELVLRPRGFFMKTCFRGTVRLY
Position: 846-876
Source molecule (GenBank): Q6ZTR5.5
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD8+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: CFAP47-WT
Chemical type: Peptide from protein
Sequence: PVTLELSSNELVLRPRGFFMKTCFRGTVRLY
Position: 846-876
Source molecule (GenBank): Q6ZTR5.5
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD4+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: RPGR-WT
Chemical type: Peptide from protein
Sequence: HGGRKEKTEILSDDLTDKAEYSASHSQIVSV
Position: 616-646
Source molecule (GenBank): Q92834-3
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD8+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: RPGR-WT
Chemical type: Peptide from protein
Sequence: HGGRKEKTEILSDDLTDKAEYSASHSQIVSV
Position: 616-646
Source molecule (GenBank): Q92834-3
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD4+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: GPR174-WT
Chemical type: Peptide from protein
Sequence: LRTSDDTPGNRTKCFVDLPTRNVNLAQSVVM
Position: 155-185
Source molecule (GenBank): XP_054183966.1
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD4+
Tissue: other
Culture conditions: Direct Ex Vivo

Epitope: GPR174-WT
Chemical type: Peptide from protein
Sequence: LRTSDDTPGNRTKCFVDLPTRNVNLAQSVVM
Position: 155-185
Source molecule (GenBank): XP_054183966.1
Assay category: TCell
Result: Negative
Host: F 61 years
Cell type: T cell CD8+
Tissue: other
Culture conditions: Direct Ex Vivo