Reference: Dan Tan; Ning Kang; Yuanfei Zhu; Jia Hou; Hanqing Wang; Huijun Xu; Cheng Zu; Zixiang Gao; Mu Liu; Nannan Liu; Qiang Deng; Hongzhou Lu; Jing Liu; Youhua Xie. "Construction and efficacy testing of DNA vaccines containing HLA-A*02:01-restricted SARS-CoV-2 T-cell epitopes predicted by immunoinformatics." Acta biochimica et biophysica Sinica (2024).

Epitope: ORF1ab1-1400aa-82
Chemical type: Peptide from protein
Sequence: VVGPNVNKGEDIQL
Position: 1117-1130
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab2787-4200aa-125
Chemical type: Peptide from protein
Sequence: KMAFPSGKVEGCMV
Position: 3268-3281
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab2787-4200aa-73
Chemical type: Peptide from protein
Sequence: KLLGVGGKPCIKVA
Position: 3843-3856
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: S-10
Chemical type: Peptide from protein
Sequence: VLYQDVNCTEVPVA
Position: 610-623
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab1387-2800aa-15
Chemical type: Peptide from protein
Sequence: INTLNDLNETLVTM
Position: 1435-1448
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab1-1400aa-19
Chemical type: Peptide from protein
Sequence: YLFDESGEFKLASH
Position: 906-919
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab1-1400aa-24
Chemical type: Peptide from protein
Sequence: KIAEIPKEEVKPFI
Position: 1202-1215
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab5783-7096aa-98
Chemical type: Peptide from protein
Sequence: MLSDTLKNLSDRVV
Position: 6094-6107
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab5783-7096aa-99
Chemical type: Peptide from protein
Sequence: AVASKILGLPTQTV
Position: 5844-5857
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: M-4
Chemical type: Peptide from protein
Sequence: FLWLLWPVTLACFV
Position: 53-66
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab1-1400aa-36
Chemical type: Peptide from protein
Sequence: KVLNEKCSAYTVEL
Position: 851-864
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab2787-4200aa-53
Chemical type: Peptide from protein
Sequence: FLCLFLLPSLATVA
Position: 3635-3648
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab4397-5796aa-29
Chemical type: Peptide from protein
Sequence: HTMLVKQGDDYVYL
Position: 5208-5221
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF7a-5
Chemical type: Peptide from protein
Sequence: ILFLALITLATCEL
Position: 4-17
Source molecule (GenBank): P0DTC7.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab1387-2800aa-43
Chemical type: Peptide from protein
Sequence: YLNSTNVTIATYCT
Position: 2270-2283
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab2787-4200aa-67
Chemical type: Peptide from protein
Sequence: VVFNGVSFSTFEEA
Position: 3165-3178
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab2787-4200aa-68
Chemical type: Peptide from protein
Sequence: IISVTSNYSGVVTT
Position: 3737-3750
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF3a-14
Chemical type: Peptide from protein
Sequence: FVRATATIPIQASL
Position: 28-41
Source molecule (GenBank): P0DTC3.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab2787-4200aa-72
Chemical type: Peptide from protein
Sequence: FLARGIVFMCVEYC
Position: 3753-3766
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: E-5
Chemical type: Peptide from protein
Sequence: RLCAYCCNIVNVSL
Position: 38-51
Source molecule (GenBank): P0DTC4.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab5783-7096aa-116
Chemical type: Peptide from protein
Sequence: FLTRNPAWRKAVFI
Position: 5823-5836
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: N28274-29533-10
Chemical type: Peptide from protein
Sequence: ALPQRQKKQQTVTL
Position: 381-394
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab1-1400aa-48
Chemical type: Peptide from protein
Sequence: KSILSPLYAFASEA
Position: 527-540
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab1387-2800aa-48
Chemical type: Peptide from protein
Sequence: VVGDIILKPANNSL
Position: 2071-2084
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab2787-4200aa-82
Chemical type: Peptide from protein
Sequence: WAQCVQLHNDILLA
Position: 3888-3901
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: S-35
Chemical type: Peptide from protein
Sequence: YICGDSTECSNLLL
Position: 741-754
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab1387-2800aa-54
Chemical type: Peptide from protein
Sequence: VAAIFYLITPVHVM
Position: 2783-2796
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab1-1400aa-52
Chemical type: Peptide from protein
Sequence: LLTPLGIDLDEWSM
Position: 889-902
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: E-8
Chemical type: Peptide from protein
Sequence: YVYSRVKNLNSSRV
Position: 57-70
Source molecule (GenBank): P0DTC4.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab1387-2800aa-60
Chemical type: Peptide from protein
Sequence: QLMCQPILLLDQAL
Position: 2563-2576
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab2787-4200aa-92
Chemical type: Peptide from protein
Sequence: YMPASWVMRIMTWL
Position: 3654-3667
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: S-40
Chemical type: Peptide from protein
Sequence: NDILSRLDKVEAEV
Position: 978-991
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab2787-4200aa-98
Chemical type: Peptide from protein
Sequence: LFFFLYENAFLPFA
Position: 3602-3615
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF7a-7
Chemical type: Peptide from protein
Sequence: RQEEVQELYSPIFL
Position: 89-102
Source molecule (GenBank): P0DTC7.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF3a-21
Chemical type: Peptide from protein
Sequence: VYSHLLLVAAGLEA
Position: 90-103
Source molecule (GenBank): P0DTC3.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: S-50
Chemical type: Peptide from protein
Sequence: FAMQMAYRFNGIGV
Position: 898-911
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab5783-7096aa-137
Chemical type: Peptide from protein
Sequence: DGVDVELFENKTTL
Position: 6488-6501
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: S-55
Chemical type: Peptide from protein
Sequence: AQKFNGLTVLPPLL
Position: 852-865
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab1387-2800aa-79
Chemical type: Peptide from protein
Sequence: KTYERHSLSHFVNL
Position: 2511-2524
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab1-1400aa-78
Chemical type: Peptide from protein
Sequence: KEGVEFLRDGWEIV
Position: 636-649
Source molecule (GenBank): P0DTC1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: S-64
Chemical type: Peptide from protein
Sequence: INLVRDLPQGFSAL
Position: 210-223
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: M-15
Chemical type: Peptide from protein
Sequence: RCDIKDLPKEITVA
Position: 158-171
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab5783-7096aa-145
Chemical type: Peptide from protein
Sequence: QQGEVPVSIINNTV
Position: 6470-6483
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: ORF1ab4397-5796aa-5
Chemical type: Peptide from protein
Sequence: ILGAGCFVDDIVKT
Position: 5229-5242
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo