Reference: Paolla B A Pinto; Maysa L Assis; Adriana L Vallochi; Agatha R Pacheco; Lauro M Lima; Kátia R L Quaresma; Bernardo A S Pereira; Simone M Costa; Ada M B Alves. "T Cell Responses Induced by DNA Vaccines Based on the DENV2 E and NS1 Proteins in Mice: Importance in Protection and Immunodominant Epitope Identification." Frontiers in immunology (2019).

Epitope: E23
Chemical type: Peptide from protein
Sequence: RFVCKHSMVDRGWGN
Position: 369-383
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E23
Chemical type: Peptide from protein
Sequence: RFVCKHSMVDRGWGN
Position: 369-383
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E23
Chemical type: Peptide from protein
Sequence: RFVCKHSMVDRGWGN
Position: 369-383
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E23
Chemical type: Peptide from protein
Sequence: RFVCKHSMVDRGWGN
Position: 369-383
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E30
Chemical type: Peptide from protein
Sequence: AMFTCKKNMKGKVVQ
Position: 397-411
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E30
Chemical type: Peptide from protein
Sequence: AMFTCKKNMKGKVVQ
Position: 397-411
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E30
Chemical type: Peptide from protein
Sequence: AMFTCKKNMKGKVVQ
Position: 397-411
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E30
Chemical type: Peptide from protein
Sequence: AMFTCKKNMKGKVVQ
Position: 397-411
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E51
Chemical type: Peptide from protein
Sequence: MENKAWLVHRQWFLD
Position: 481-495
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E51
Chemical type: Peptide from protein
Sequence: MENKAWLVHRQWFLD
Position: 481-495
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E51
Chemical type: Peptide from protein
Sequence: MENKAWLVHRQWFLD
Position: 481-495
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E51
Chemical type: Peptide from protein
Sequence: MENKAWLVHRQWFLD
Position: 481-495
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E60
Chemical type: Peptide from protein
Sequence: LVTFKNPHAKKQDVV
Position: 517-531
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E60
Chemical type: Peptide from protein
Sequence: LVTFKNPHAKKQDVV
Position: 517-531
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Positive
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E60
Chemical type: Peptide from protein
Sequence: LVTFKNPHAKKQDVV
Position: 517-531
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E60
Chemical type: Peptide from protein
Sequence: LVTFKNPHAKKQDVV
Position: 517-531
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E66
Chemical type: Peptide from protein
Sequence: HTALTGATEIQMSSG
Position: 541-555
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E66
Chemical type: Peptide from protein
Sequence: HTALTGATEIQMSSG
Position: 541-555
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E66
Chemical type: Peptide from protein
Sequence: HTALTGATEIQMSSG
Position: 541-555
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E66
Chemical type: Peptide from protein
Sequence: HTALTGATEIQMSSG
Position: 541-555
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Positive
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E67
Chemical type: Peptide from protein
Sequence: TGATEIQMSSGNLLF
Position: 545-559
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E67
Chemical type: Peptide from protein
Sequence: TGATEIQMSSGNLLF
Position: 545-559
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Positive
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E67
Chemical type: Peptide from protein
Sequence: TGATEIQMSSGNLLF
Position: 545-559
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E67
Chemical type: Peptide from protein
Sequence: TGATEIQMSSGNLLF
Position: 545-559
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E76
Chemical type: Peptide from protein
Sequence: MCTGKFKVVKEIAET
Position: 581-595
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E76
Chemical type: Peptide from protein
Sequence: MCTGKFKVVKEIAET
Position: 581-595
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E76
Chemical type: Peptide from protein
Sequence: MCTGKFKVVKEIAET
Position: 581-595
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E76
Chemical type: Peptide from protein
Sequence: MCTGKFKVVKEIAET
Position: 581-595
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E89
Chemical type: Peptide from protein
Sequence: TVNPIVTEKDSPVNI
Position: 633-647
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E89
Chemical type: Peptide from protein
Sequence: TVNPIVTEKDSPVNI
Position: 633-647
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E89
Chemical type: Peptide from protein
Sequence: TVNPIVTEKDSPVNI
Position: 633-647
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E89
Chemical type: Peptide from protein
Sequence: TVNPIVTEKDSPVNI
Position: 633-647
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS39
Chemical type: Peptide from protein
Sequence: LEVEDYGFGVFTTNI
Position: 928-942
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS39
Chemical type: Peptide from protein
Sequence: LEVEDYGFGVFTTNI
Position: 928-942
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS39
Chemical type: Peptide from protein
Sequence: LEVEDYGFGVFTTNI
Position: 928-942
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS39
Chemical type: Peptide from protein
Sequence: LEVEDYGFGVFTTNI
Position: 928-942
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS44
Chemical type: Peptide from protein
Sequence: EKQDVFCDSKLMSAA
Position: 948-962
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS44
Chemical type: Peptide from protein
Sequence: EKQDVFCDSKLMSAA
Position: 948-962
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS44
Chemical type: Peptide from protein
Sequence: EKQDVFCDSKLMSAA
Position: 948-962
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS44
Chemical type: Peptide from protein
Sequence: EKQDVFCDSKLMSAA
Position: 948-962
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS47
Chemical type: Peptide from protein
Sequence: SAAIKDNRAVHADMG
Position: 960-974
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS47
Chemical type: Peptide from protein
Sequence: SAAIKDNRAVHADMG
Position: 960-974
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS47
Chemical type: Peptide from protein
Sequence: SAAIKDNRAVHADMG
Position: 960-974
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS47
Chemical type: Peptide from protein
Sequence: SAAIKDNRAVHADMG
Position: 960-974
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS57
Chemical type: Peptide from protein
Sequence: WPKSHTLWSNGVLES
Position: 1000-1014
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS57
Chemical type: Peptide from protein
Sequence: WPKSHTLWSNGVLES
Position: 1000-1014
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS57
Chemical type: Peptide from protein
Sequence: WPKSHTLWSNGVLES
Position: 1000-1014
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS57
Chemical type: Peptide from protein
Sequence: WPKSHTLWSNGVLES
Position: 1000-1014
Source molecule (GenBank): AAA42941.1
Assay category: TCell
Result: Negative
Host:  4 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo