Reference: Luca Hensen; Patricia T Illing; E Bridie Clemens; Thi H O Nguyen; Marios Koutsakos; Carolien E van de Sandt; Nicole A Mifsud; Andrea T Nguyen; Christopher Szeto; Brendon Y Chua; Hanim Halim; Simone Rizzetto; Fabio Luciani; Liyen Loh; Emma J Grant; Phillipa M Saunders; Andrew G Brooks; Steve Rockman; Tom C Kotsimbos; Allen C Cheng; Michael Richards; Glen P Westall; Linda M Wakim; Thomas Loudovaris; Stuart I Mannering; Michael Elliott; Stuart G Tangye; David C Jackson; Katie L Flanagan; Jamie Rossjohn; Stephanie Gras; Jane Davies; Adrian Miller; Steven Y C Tong; Anthony W Purcell; Katherine Kedzierska. "CD8<sup>+</sup> T cell landscape in Indigenous and non-Indigenous people restricted by influenza mortality-associated HLA-A*24:02 allomorph." Nature communications (2021).

Epitope: M1-239-248
Chemical type: Peptide from protein
Sequence: AYQKRMGVQM
Position: 239-248
Source molecule (GenBank): ACU79927.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: bronchial aveolar lavage (BAL)
Culture conditions: Direct Ex Vivo

Epitope: M1-239-248
Chemical type: Peptide from protein
Sequence: AYQKRMGVQM
Position: 239-248
Source molecule (GenBank): ACU79927.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: bronchial aveolar lavage (BAL)
Culture conditions: Direct Ex Vivo

Epitope: HA-176-184-mod-M9
Chemical type: Peptide from protein
Sequence: TYPVLNVTM
Position: 176-184
Source molecule (GenBank): ACU79926.1
Assay category: MhcLigandElution
Result: Positive

Epitope: NP-138-148
Chemical type: Peptide from protein
Sequence: IWHSNLNDATY
Position: 138-148
Source molecule (GenBank): AAW78291.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: bronchial aveolar lavage (BAL)
Culture conditions: Direct Ex Vivo

Epitope: NP-138-148
Chemical type: Peptide from protein
Sequence: IWHSNLNDATY
Position: 138-148
Source molecule (GenBank): AAW78291.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: bronchial aveolar lavage (BAL)
Culture conditions: Direct Ex Vivo

Epitope: NP-138-148
Chemical type: Peptide from protein
Sequence: IWHSNLNDATY
Position: 138-148
Source molecule (GenBank): AAW78291.1
Assay category: MhcLigandElution
Result: Positive

Epitope: NP-257-265
Chemical type: Peptide from protein
Sequence: TFLARSALI
Position: 257-265
Source molecule (GenBank): ADL60243.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: bronchial aveolar lavage (BAL)
Culture conditions: Direct Ex Vivo

Epitope: NP-257-265
Chemical type: Peptide from protein
Sequence: TFLARSALI
Position: 257-265
Source molecule (GenBank): ADL60243.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: bronchial aveolar lavage (BAL)
Culture conditions: Direct Ex Vivo

Epitope: NP-257-265
Chemical type: Peptide from protein
Sequence: TFLARSALI
Position: 257-265
Source molecule (GenBank): ADL60243.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: NP-257-265
Chemical type: Peptide from protein
Sequence: TFLARSALI
Position: 257-265
Source molecule (GenBank): ADL60243.1
Assay category: MhcLigandElution
Result: Positive

Epitope: NS2-98-106-mod-M3
Chemical type: Peptide from protein
Sequence: TFMQALHLL
Position: 98-106
Source molecule (GenBank): ACI26302.1
Assay category: MhcLigandElution
Result: Positive

Epitope: PA-47-60-mod-M1, Q11
Chemical type: Peptide from protein
Sequence: MYSDFHFINEQGES
Position: 47-60
Source molecule (GenBank): ALH28214.1
Assay category: MhcLigandElution
Result: Positive-Low

Epitope: PB2-227-234
Chemical type: Peptide from protein
Sequence: VYIEVLHL
Position: 227-234
Source molecule (GenBank): AID57819.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: PB2-227-234
Chemical type: Peptide from protein
Sequence: VYIEVLHL
Position: 227-234
Source molecule (GenBank): AID57819.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: bronchial aveolar lavage (BAL)
Culture conditions: Direct Ex Vivo

Epitope: PB2-227-234
Chemical type: Peptide from protein
Sequence: VYIEVLHL
Position: 227-234
Source molecule (GenBank): AID57819.1
Assay category: TCell
Result: Positive-Low
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: bronchial aveolar lavage (BAL)
Culture conditions: Direct Ex Vivo

Epitope: PB2-227-234
Chemical type: Peptide from protein
Sequence: VYIEVLHL
Position: 227-234
Source molecule (GenBank): AID57819.1
Assay category: MhcLigandElution
Result: Positive

Epitope: PB2-549-557-mod-W4
Chemical type: Peptide from protein
Sequence: TYQWIIRNW
Position: 549-557
Source molecule (GenBank): ABI26656.1
Assay category: MhcLigandElution
Result: Positive

Epitope: NP-473-481-mod-M9
Chemical type: Peptide from protein
Sequence: SPIVPSFDM
Position: 479-487
Source molecule (GenBank): ABY70960.1
Assay category: MhcLigandElution
Result: Positive

Epitope: PB2-57-66-mod-M10
Chemical type: Peptide from protein
Sequence: ITADKRITEM
Position: 57-66
Source molecule (GenBank): ABI26656.1
Assay category: MhcLigandElution
Result: Positive-Low

Epitope: PB1-22-30
Chemical type: Peptide from protein
Sequence: FPYTGDPPY
Position: 22-30
Source molecule (GenBank): AAX47533.1
Assay category: MhcLigandElution
Result: Positive

Epitope: PB2-199-207-mod-S1
Chemical type: Peptide from protein
Sequence: SPLMVAYML
Position: 199-207
Source molecule (GenBank): ABI26656.1
Assay category: MhcLigandElution
Result: Positive-Low

Epitope: HA-254-268
Chemical type: Peptide from protein
Sequence: KPGDVLVINSNGNLI
Position: 254-268
Source molecule (GenBank): ACU79926.1
Assay category: MhcLigandElution
Result: Positive

Epitope: HA-254-271-mod-N9, N11
Chemical type: Peptide from protein
Sequence: KPGDVLVINSNGNLIAPR
Position: 254-271
Source molecule (GenBank): ACU79926.1
Assay category: MhcLigandElution
Result: Positive

Epitope: HA-288-309-mod-C10, P13
Chemical type: Peptide from protein
Sequence: APIDTCISECITPNGSIPNDKP
Position: 288-309
Source molecule (GenBank): ACU79926.1
Assay category: MhcLigandElution
Result: Positive-Low

Epitope: HA-296-310-mod-C2
Chemical type: Peptide from protein
Sequence: ECITPNGSIPNDKPF
Position: 296-310
Source molecule (GenBank): ACU79926.1
Assay category: MhcLigandElution
Result: Positive-Low

Epitope: NA-298-311-mod-M10
Chemical type: Peptide from protein
Sequence: SNRPVVDINMEDYS
Position: 298-311
Source molecule (GenBank): AAK51727.1
Assay category: MhcLigandElution
Result: Positive

Epitope: NP-408-420
Chemical type: Peptide from protein
Sequence: IQPTFSVQRNLPF
Position: 408-420
Source molecule (GenBank): ABH05853.1
Assay category: MhcLigandElution
Result: Positive

Epitope: NP-408-421
Chemical type: Peptide from protein
Sequence: IQPTFSVQRNLPFD
Position: 408-421
Source molecule (GenBank): ABH05853.1
Assay category: MhcLigandElution
Result: Positive

Epitope: NP-409-422
Chemical type: Peptide from protein
Sequence: QPTFSVQRNLPFDR
Position: 409-422
Source molecule (GenBank): ABH05853.1
Assay category: MhcLigandElution
Result: Positive-Low

Epitope: M1-131-140-mod-M10
Chemical type: Peptide from protein
Sequence: MYLNPGNYSM
Position: 131-140
Source molecule (GenBank): ANE29705.1
Assay category: MhcLigandElution
Result: Positive

Epitope: NA-213-221
Chemical type: Peptide from protein
Sequence: TYHSYANNI
Position: 213-221
Source molecule (GenBank): ACO05961.1
Assay category: TCell
Result: Positive-Low
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: bronchial aveolar lavage (BAL)
Culture conditions: Direct Ex Vivo

Epitope: NA-213-221
Chemical type: Peptide from protein
Sequence: TYHSYANNI
Position: 213-221
Source molecule (GenBank): ACO05961.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: bronchial aveolar lavage (BAL)
Culture conditions: Direct Ex Vivo

Epitope: NA-213-221
Chemical type: Peptide from protein
Sequence: TYHSYANNI
Position: 213-221
Source molecule (GenBank): ACO05961.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: NA-213-221
Chemical type: Peptide from protein
Sequence: TYHSYANNI
Position: 213-221
Source molecule (GenBank): ACO05961.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: NA-213-221
Chemical type: Peptide from protein
Sequence: TYHSYANNI
Position: 213-221
Source molecule (GenBank): ACO05961.1
Assay category: MhcLigandElution
Result: Positive-Low

Epitope: NA-458-466
Chemical type: Peptide from protein
Sequence: TVTGVNMAL
Position: 458-466
Source molecule (GenBank): ACO05961.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: bronchial aveolar lavage (BAL)
Culture conditions: Direct Ex Vivo

Epitope: NA-458-466
Chemical type: Peptide from protein
Sequence: TVTGVNMAL
Position: 458-466
Source molecule (GenBank): ACO05961.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: bronchial aveolar lavage (BAL)
Culture conditions: Direct Ex Vivo

Epitope: NA-458-466
Chemical type: Peptide from protein
Sequence: TVTGVNMAL
Position: 458-466
Source molecule (GenBank): ACO05961.1
Assay category: MhcLigandElution
Result: Positive-Low

Epitope: NP-181-191-mod-M1
Chemical type: Peptide from protein
Sequence: MYKTTMGSDGF
Position: 181-191
Source molecule (GenBank): AOT97599.1
Assay category: MhcLigandElution
Result: Positive

Epitope: NP-181-191
Chemical type: Peptide from protein
Sequence: MYKTTMGSDGF
Position: 181-191
Source molecule (GenBank): AOT97599.1
Assay category: MhcLigandElution
Result: Positive

Epitope: NP-399-408
Chemical type: Peptide from protein
Sequence: VLSALTGTEF
Position: 399-408
Source molecule (GenBank): AOT97599.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: bronchial aveolar lavage (BAL)
Culture conditions: Direct Ex Vivo

Epitope: NP-399-408
Chemical type: Peptide from protein
Sequence: VLSALTGTEF
Position: 399-408
Source molecule (GenBank): AOT97599.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: bronchial aveolar lavage (BAL)
Culture conditions: Direct Ex Vivo

Epitope: NP-399-408
Chemical type: Peptide from protein
Sequence: VLSALTGTEF
Position: 399-408
Source molecule (GenBank): AOT97599.1
Assay category: MhcLigandElution
Result: Positive

Epitope: PA-453-461-mod-M4
Chemical type: Peptide from protein
Sequence: TVMMKYVLF
Position: 453-461
Source molecule (GenBank): ARS00018.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: bronchial aveolar lavage (BAL)
Culture conditions: Direct Ex Vivo

Epitope: PA-453-461-mod-M4
Chemical type: Peptide from protein
Sequence: TVMMKYVLF
Position: 453-461
Source molecule (GenBank): ARS00018.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: bronchial aveolar lavage (BAL)
Culture conditions: Direct Ex Vivo

Epitope: PA-453-461-mod-M4
Chemical type: Peptide from protein
Sequence: TVMMKYVLF
Position: 453-461
Source molecule (GenBank): ARS00018.1
Assay category: MhcLigandElution
Result: Positive-Low

Epitope: HA-460-475
Chemical type: Peptide from protein
Sequence: LAVLLSNEGIINSEDE
Position: 455-470
Source molecule (GenBank): ABU99194.1
Assay category: MhcLigandElution
Result: Positive

Epitope: PA-47-53
Chemical type: Peptide from protein
Sequence: MYSDFHF
Position: 47-53
Source molecule (GenBank): AIS23591.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: bronchial aveolar lavage (BAL)
Culture conditions: Direct Ex Vivo

Epitope: PA-47-53
Chemical type: Peptide from protein
Sequence: MYSDFHF
Position: 47-53
Source molecule (GenBank): AIS23591.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: bronchial aveolar lavage (BAL)
Culture conditions: Direct Ex Vivo

Epitope: PA-47-53
Chemical type: Peptide from protein
Sequence: MYSDFHF
Position: 47-53
Source molecule (GenBank): AIS23591.1
Assay category: MhcLigandElution
Result: Positive

Epitope: PA-146-156
Chemical type: Peptide from protein
Sequence: MIFSYNQDYSL
Position: 146-156
Source molecule (GenBank): AGZ59995.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: bronchial aveolar lavage (BAL)
Culture conditions: Direct Ex Vivo

Epitope: PA-146-156
Chemical type: Peptide from protein
Sequence: MIFSYNQDYSL
Position: 146-156
Source molecule (GenBank): AGZ59995.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: bronchial aveolar lavage (BAL)
Culture conditions: Direct Ex Vivo

Epitope: PA-146-156
Chemical type: Peptide from protein
Sequence: MIFSYNQDYSL
Position: 146-156
Source molecule (GenBank): AGZ59995.1
Assay category: MhcLigandElution
Result: Positive

Epitope: PB2-433-441
Chemical type: Peptide from protein
Sequence: MYQLQRYFL
Position: 433-441
Source molecule (GenBank): ACO05967.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: bronchial aveolar lavage (BAL)
Culture conditions: Direct Ex Vivo

Epitope: PB2-433-441
Chemical type: Peptide from protein
Sequence: MYQLQRYFL
Position: 433-441
Source molecule (GenBank): ACO05967.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: PB2-433-441
Chemical type: Peptide from protein
Sequence: MYQLQRYFL
Position: 433-441
Source molecule (GenBank): ACO05967.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: PB2-433-441
Chemical type: Peptide from protein
Sequence: MYQLQRYFL
Position: 433-441
Source molecule (GenBank): ACO05967.1
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: bronchial aveolar lavage (BAL)
Culture conditions: Direct Ex Vivo

Epitope: PB2-433-441
Chemical type: Peptide from protein
Sequence: MYQLQRYFL
Position: 433-441
Source molecule (GenBank): ACO05967.1
Assay category: MhcLigandElution
Result: Positive