Reference: Nathan P Croft; Stewart A Smith; Jana Pickering; John Sidney; Bjoern Peters; Pouya Faridi; Matthew J Witney; Prince Sebastian; Inge E A Flesch; Sally L Heading; Alessandro Sette; Nicole L La Gruta; Anthony W Purcell; David C Tscharke. "Most viral peptides displayed by class I MHC on infected cells are immunogenic." Proceedings of the National Academy of Sciences of the United States of America (2019).

Epitope: WR148 (257-264)
Chemical type: Peptide from protein
Sequence: SIYQYVRL
Position: 257-264
Source molecule (GenBank): SOU90160.1
Assay category: TCell
Result: Positive
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: WR148 (257-264)
Chemical type: Peptide from protein
Sequence: SIYQYVRL
Position: 257-264
Source molecule (GenBank): SOU90160.1
Assay category: MhcLigandElution
Result: Positive

Epitope: C4 (125-132)
Chemical type: Peptide from protein
Sequence: LNFRFENV
Position: 125-132
Source molecule (GenBank): P17370.2
Assay category: TCell
Result: Positive
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: C4 (125-132)
Chemical type: Peptide from protein
Sequence: LNFRFENV
Position: 125-132
Source molecule (GenBank): P17370.2
Assay category: MhcLigandElution
Result: Positive

Epitope: A8 (214-222)
Chemical type: Peptide from protein
Sequence: NQMVNTVLI
Position: 214-222
Source molecule (GenBank): P68720.1
Assay category: TCell
Result: Positive
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A8 (214-222)
Chemical type: Peptide from protein
Sequence: NQMVNTVLI
Position: 214-222
Source molecule (GenBank): P68720.1
Assay category: MhcLigandElution
Result: Positive

Epitope: A8 (214-222)
Chemical type: Peptide from protein
Sequence: NQMVNTVLI
Position: 214-222
Source molecule (GenBank): P68720.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 0.5

Epitope: A47 (94-101)
Chemical type: Peptide from protein
Sequence: MMINPFMI
Position: 94-101
Source molecule (GenBank): P26673.2
Assay category: TCell
Result: Positive
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A47 (94-101)
Chemical type: Peptide from protein
Sequence: MMINPFMI
Position: 94-101
Source molecule (GenBank): P26673.2
Assay category: MhcLigandElution
Result: Positive

Epitope: A47 (94-101)
Chemical type: Peptide from protein
Sequence: MMINPFMI
Position: 94-101
Source molecule (GenBank): P26673.2
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 1096.5

Epitope: D1 (578-586)
Chemical type: Peptide from protein
Sequence: SMYCSKTFL
Position: 578-586
Source molecule (GenBank): P04298.1
Assay category: TCell
Result: Positive
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D13 (118-126)
Chemical type: Peptide from protein
Sequence: NCINNTIAL
Position: 118-126
Source molecule (GenBank): P68440.1
Assay category: TCell
Result: Positive
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: C4 (119-127)
Chemical type: Peptide from protein
Sequence: IMFSKSLNF
Position: 119-127
Source molecule (GenBank): P17370.2
Assay category: TCell
Result: Positive
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: C4 (119-127)
Chemical type: Peptide from protein
Sequence: IMFSKSLNF
Position: 119-127
Source molecule (GenBank): P17370.2
Assay category: MhcLigandElution
Result: Positive

Epitope: C4 (119-127)
Chemical type: Peptide from protein
Sequence: IMFSKSLNF
Position: 119-127
Source molecule (GenBank): P17370.2
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.323098

Epitope: J4 (110-116)
Chemical type: Peptide from protein
Sequence: FPSFVRL
Position: 110-116
Source molecule (GenBank): P68609.1
Assay category: TCell
Result: Positive
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: J4 (110-116)
Chemical type: Peptide from protein
Sequence: FPSFVRL
Position: 110-116
Source molecule (GenBank): P68609.1
Assay category: MhcLigandElution
Result: Positive

Epitope: J4 (110-116)
Chemical type: Peptide from protein
Sequence: FPSFVRL
Position: 110-116
Source molecule (GenBank): P68609.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 43.339317

Epitope: B4 (48-56)
Chemical type: Peptide from protein
Sequence: TIVDFLSRL
Position: 48-56
Source molecule (GenBank): P24769.1
Assay category: TCell
Result: Positive
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B4 (48-56)
Chemical type: Peptide from protein
Sequence: TIVDFLSRL
Position: 48-56
Source molecule (GenBank): P24769.1
Assay category: MhcLigandElution
Result: Positive

Epitope: B4 (48-56)
Chemical type: Peptide from protein
Sequence: TIVDFLSRL
Position: 48-56
Source molecule (GenBank): P24769.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 0.5

Epitope: N2 (60-68)
Chemical type: Peptide from protein
Sequence: FLMMNKDEL
Position: 60-68
Source molecule (GenBank): P14357.1
Assay category: TCell
Result: Positive
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N2 (60-68)
Chemical type: Peptide from protein
Sequence: FLMMNKDEL
Position: 60-68
Source molecule (GenBank): P14357.1
Assay category: MhcLigandElution
Result: Positive

Epitope: A10 (265-272)
Chemical type: Peptide from protein
Sequence: FIYTYDRV
Position: 265-272
Source molecule (GenBank): P16715.1
Assay category: TCell
Result: Positive
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A18 (247-255)
Chemical type: Peptide from protein
Sequence: NSIINIAKL
Position: 247-255
Source molecule (GenBank): P16712.1
Assay category: TCell
Result: Positive
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A18 (247-255)
Chemical type: Peptide from protein
Sequence: NSIINIAKL
Position: 247-255
Source molecule (GenBank): P16712.1
Assay category: MhcLigandElution
Result: Positive

Epitope: A18 (247-255)
Chemical type: Peptide from protein
Sequence: NSIINIAKL
Position: 247-255
Source molecule (GenBank): P16712.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 58.336346

Epitope: E2 (616-624)
Chemical type: Peptide from protein
Sequence: NAVSNKYEL
Position: 616-624
Source molecule (GenBank): P21604.2
Assay category: TCell
Result: Positive
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E2 (616-624)
Chemical type: Peptide from protein
Sequence: NAVSNKYEL
Position: 616-624
Source molecule (GenBank): P21604.2
Assay category: MhcLigandElution
Result: Positive

Epitope: E2 (616-624)
Chemical type: Peptide from protein
Sequence: NAVSNKYEL
Position: 616-624
Source molecule (GenBank): P21604.2
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 0.79061

Epitope: L1 (139-149)
Chemical type: Peptide from protein
Sequence: APGSPTNLEFI
Position: 139-149
Source molecule (GenBank): P07612.3
Assay category: TCell
Result: Positive
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: L1 (139-149)
Chemical type: Peptide from protein
Sequence: APGSPTNLEFI
Position: 139-149
Source molecule (GenBank): P07612.3
Assay category: MhcLigandElution
Result: Positive

Epitope: L1 (139-149)
Chemical type: Peptide from protein
Sequence: APGSPTNLEFI
Position: 139-149
Source molecule (GenBank): P07612.3
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 14.039517

Epitope: A20 (372-381)
Chemical type: Peptide from protein
Sequence: VNNMRFKIEN
Position: 372-381
Source molecule (GenBank): P68710.1
Assay category: TCell
Result: Positive
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A20 (372-381)
Chemical type: Peptide from protein
Sequence: VNNMRFKIEN
Position: 372-381
Source molecule (GenBank): P68710.1
Assay category: MhcLigandElution
Result: Positive

Epitope: A20 (372-381)
Chemical type: Peptide from protein
Sequence: VNNMRFKIEN
Position: 372-381
Source molecule (GenBank): P68710.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 98.27774

Epitope: J6 (729-736)
Chemical type: Peptide from protein
Sequence: SQYYFSML
Position: 729-736
Source molecule (GenBank): P07392.1
Assay category: TCell
Result: Positive
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: J6 (729-736)
Chemical type: Peptide from protein
Sequence: SQYYFSML
Position: 729-736
Source molecule (GenBank): P07392.1
Assay category: MhcLigandElution
Result: Positive

Epitope: J6 (729-736)
Chemical type: Peptide from protein
Sequence: SQYYFSML
Position: 729-736
Source molecule (GenBank): P07392.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 0.5

Epitope: E4 (96-103)
Chemical type: Peptide from protein
Sequence: TNLEYKTL
Position: 96-103
Source molecule (GenBank): P21603.1
Assay category: TCell
Result: Positive
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E4 (96-103)
Chemical type: Peptide from protein
Sequence: TNLEYKTL
Position: 96-103
Source molecule (GenBank): P21603.1
Assay category: MhcLigandElution
Result: Positive

Epitope: E4 (96-103)
Chemical type: Peptide from protein
Sequence: TNLEYKTL
Position: 96-103
Source molecule (GenBank): P21603.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 2.950779

Epitope: E ORF A (43-51)
Chemical type: Peptide from protein
Sequence: LLPSHSTVL
Position: 43-51
Source molecule (GenBank): AGJ92030.1
Assay category: TCell
Result: Positive
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E2 (305-312)
Chemical type: Peptide from protein
Sequence: YIDHIVNI
Position: 305-312
Source molecule (GenBank): P21604.2
Assay category: TCell
Result: Positive
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E2 (305-312)
Chemical type: Peptide from protein
Sequence: YIDHIVNI
Position: 305-312
Source molecule (GenBank): P21604.2
Assay category: MhcLigandElution
Result: Positive

Epitope: E2 (305-312)
Chemical type: Peptide from protein
Sequence: YIDHIVNI
Position: 305-312
Source molecule (GenBank): P21604.2
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 17794.139

Epitope: I3 (41-48)
Chemical type: Peptide from protein
Sequence: KCIEYVTL
Position: 41-48
Source molecule (GenBank): P12923.1
Assay category: TCell
Result: Positive
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: I3 (41-48)
Chemical type: Peptide from protein
Sequence: KCIEYVTL
Position: 41-48
Source molecule (GenBank): P12923.1
Assay category: MhcLigandElution
Result: Positive

Epitope: I3 (41-48)
Chemical type: Peptide from protein
Sequence: KCIEYVTL
Position: 41-48
Source molecule (GenBank): P12923.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 808.1674

Epitope: E9 (200-208)
Chemical type: Peptide from protein
Sequence: FTLINEEML
Position: 200-208
Source molecule (GenBank): AAO89344.1
Assay category: TCell
Result: Positive
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E9 (200-208)
Chemical type: Peptide from protein
Sequence: FTLINEEML
Position: 200-208
Source molecule (GenBank): AAO89344.1
Assay category: MhcLigandElution
Result: Positive

Epitope: E9 (200-208)
Chemical type: Peptide from protein
Sequence: FTLINEEML
Position: 200-208
Source molecule (GenBank): AAO89344.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 7.495429

Epitope: E9 (766-774)
Chemical type: Peptide from protein
Sequence: KSIEIAKEL
Position: 766-774
Source molecule (GenBank): AAO89344.1
Assay category: TCell
Result: Positive
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E9 (766-774)
Chemical type: Peptide from protein
Sequence: KSIEIAKEL
Position: 766-774
Source molecule (GenBank): AAO89344.1
Assay category: MhcLigandElution
Result: Positive

Epitope: E9 (766-774)
Chemical type: Peptide from protein
Sequence: KSIEIAKEL
Position: 766-774
Source molecule (GenBank): AAO89344.1
Assay category: MhcBinding
Result: Positive
Quantitative measurement: 3614.0972

Epitope: K ORF B (27-34)
Chemical type: Peptide from protein
Sequence: IPLKYIVL
Position: 48-55
Source molecule (GenBank): AAW23428.1
Assay category: TCell
Result: Positive
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: I7 (129-136)
Chemical type: Peptide from protein
Sequence: KAIDFSQM
Position: 129-136
Source molecule (GenBank): P12926.1
Assay category: TCell
Result: Negative
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: I7 (129-136)
Chemical type: Peptide from protein
Sequence: KAIDFSQM
Position: 129-136
Source molecule (GenBank): P12926.1
Assay category: MhcLigandElution
Result: Positive

Epitope: I7 (129-136)
Chemical type: Peptide from protein
Sequence: KAIDFSQM
Position: 129-136
Source molecule (GenBank): P12926.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 11.378092

Epitope: D5 (3-11)
Chemical type: Peptide from protein
Sequence: AAIRGNDVI
Position: 3-11
Source molecule (GenBank): P04305.1
Assay category: TCell
Result: Negative
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D5 (3-11)
Chemical type: Peptide from protein
Sequence: AAIRGNDVI
Position: 3-11
Source molecule (GenBank): P04305.1
Assay category: MhcLigandElution
Result: Positive

Epitope: D5 (3-11)
Chemical type: Peptide from protein
Sequence: AAIRGNDVI
Position: 3-11
Source molecule (GenBank): P04305.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 10.750496

Epitope: L1 (158-166)
Chemical type: Peptide from protein
Sequence: CAIKALMQL
Position: 158-166
Source molecule (GenBank): P07612.3
Assay category: TCell
Result: Negative
Host: F 8-16 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo