Reference: B van Denderen; H Pêche; K Gagne; C Usal; M C Cuturi; J P Soulillou. "Identification of immunodominant donor MHC peptides following rejection and donor strain transfusion-induced tolerance of heart allografts in adult rats." European journal of immunology (2001).

Epitope: Peptide 45 RT1<sup>u</sup>
Chemical type: Peptide from protein
Sequence: LAAALAQTQTRAGSHS
Position: 16-31
Source molecule (GenBank): AAM90626.1
Assay category: TCell
Result: Negative
Host: M 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide 45 RT1<sup>u</sup>
Chemical type: Peptide from protein
Sequence: LAAALAQTQTRAGSHS
Position: 16-31
Source molecule (GenBank): AAM90626.1
Assay category: TCell
Result: Negative
Host: M 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide 51 RT1<sup>u</sup>
Chemical type: Peptide from protein
Sequence: GHEQVNRVNLRTLRGY
Position: 90-105
Source molecule (GenBank): AAC52552.1
Assay category: TCell
Result: Negative
Host: M 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide 51 RT1<sup>u</sup>
Chemical type: Peptide from protein
Sequence: GHEQVNRVNLRTLRGY
Position: 90-105
Source molecule (GenBank): AAC52552.1
Assay category: TCell
Result: Negative
Host: M 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide 58 RT1<sup>u</sup>
Chemical type: Peptide from protein
Sequence: ADYYKAYLEGTCLESL
Position: 174-189
Source molecule (GenBank): AAC52552.1
Assay category: TCell
Result: Positive
Host: M 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide 58 RT1<sup>u</sup>
Chemical type: Peptide from protein
Sequence: ADYYKAYLEGTCLESL
Position: 174-189
Source molecule (GenBank): AAC52552.1
Assay category: TCell
Result: Positive
Host: M 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide 61 RT1<sup>u</sup>
Chemical type: Peptide from protein
Sequence: LESLRRYLELGKERLL
Position: 186-201
Source molecule (GenBank): AAC52552.1
Assay category: TCell
Result: Negative
Host: M 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide 61 RT1<sup>u</sup>
Chemical type: Peptide from protein
Sequence: LESLRRYLELGKERLL
Position: 186-201
Source molecule (GenBank): AAC52552.1
Assay category: TCell
Result: Negative
Host: M 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide 13 RT1<sup>u</sup>
Chemical type: Peptide from protein
Sequence: YLRYDSDVGEYRAVTE
Position: 37-52
Source molecule (GenBank): AAA41604.1
Assay category: TCell
Result: Negative
Host: M 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide 13 RT1<sup>u</sup>
Chemical type: Peptide from protein
Sequence: YLRYDSDVGEYRAVTE
Position: 37-52
Source molecule (GenBank): AAA41604.1
Assay category: TCell
Result: Negative
Host: M 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide 17 RT1<sup>u</sup>
Chemical type: Peptide from protein
Sequence: LGRPSAEYFNKQYLER
Position: 53-68
Source molecule (GenBank): AAA41604.1
Assay category: TCell
Result: Negative
Host: M 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide 17 RT1<sup>u</sup>
Chemical type: Peptide from protein
Sequence: LGRPSAEYFNKQYLER
Position: 53-68
Source molecule (GenBank): AAA41604.1
Assay category: TCell
Result: Negative
Host: M 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide 21 RT1<sup>u</sup>
Chemical type: Peptide from protein
Sequence: TRAELDTVCRHNYEKT
Position: 69-84
Source molecule (GenBank): AAA41604.1
Assay category: TCell
Result: Negative
Host: M 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide 21 RT1<sup>u</sup>
Chemical type: Peptide from protein
Sequence: TRAELDTVCRHNYEKT
Position: 69-84
Source molecule (GenBank): AAA41604.1
Assay category: TCell
Result: Positive
Host: M 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide 23 RT1<sup>u</sup>
Chemical type: Peptide from protein
Sequence: CRHNYEKTEVPTSLRR
Position: 77-92
Source molecule (GenBank): AAA41604.1
Assay category: TCell
Result: Negative
Host: M 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide 23 RT1<sup>u</sup>
Chemical type: Peptide from protein
Sequence: CRHNYEKTEVPTSLRR
Position: 77-92
Source molecule (GenBank): AAA41604.1
Assay category: TCell
Result: Negative
Host: M 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide 42 RT1<sup>u</sup>
Chemical type: Peptide from protein
Sequence: TYCRHNYEIFDRFLVP
Position: 104-119
Source molecule (GenBank): AAD39084.1
Assay category: TCell
Result: Positive
Host: M 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide 42 RT1<sup>u</sup>
Chemical type: Peptide from protein
Sequence: TYCRHNYEIFDRFLVP
Position: 104-119
Source molecule (GenBank): AAD39084.1
Assay category: TCell
Result: Negative
Host: M 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide 43 RT1<sup>u</sup>
Chemical type: Peptide from protein
Sequence: HNYEIFDRFLVPRRVE
Position: 108-123
Source molecule (GenBank): AAD39084.1
Assay category: TCell
Result: Negative
Host: M 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide 43 RT1<sup>u</sup>
Chemical type: Peptide from protein
Sequence: HNYEIFDRFLVPRRVE
Position: 108-123
Source molecule (GenBank): AAD39084.1
Assay category: TCell
Result: Negative
Host: M 6-7 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo