Reference: Hangjie Zhang; Wenling Xiao; Min Zhao; Yingze Zhao; Yongli Zhang; Dan Lu; Shuangshuang Lu; Qingxu Zhang; Weiyu Peng; Liumei Shu; Jie Zhang; Sai Liu; Kexin Zong; Pengyan Wang; Beiwei Ye; Shihua Li; Shuguang Tan; Fuping Zhang; Jianfang Zhou; Peipei Liu; Guizhen Wu; Xuancheng Lu; George F Gao; William J Liu. "The CD8+ and CD4+ T Cell Immunogen Atlas of Zika Virus Reveals E, NS1 and NS4 Proteins as the Vaccine Targets." Viruses (2022).

Epitope: SNRDFVEGM
Chemical type: Peptide from protein
Sequence: SNRDFVEGM
Position: 297-305
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Intermediate
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: VLGSQEGAV
Chemical type: Peptide from protein
Sequence: VLGSQEGAV
Position: 547-555
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS2 1479-1486
Chemical type: Peptide from protein
Sequence: CGMNPIAI
Position: 1479-1486
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-High
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS2 1479-1486
Chemical type: Peptide from protein
Sequence: CGMNPIAI
Position: 1479-1486
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-High
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: YAWDFGVPL
Chemical type: Peptide from protein
Sequence: YAWDFGVPL
Position: 2356-2364
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: CNIFRGSYL
Chemical type: Peptide from protein
Sequence: CNIFRGSYL
Position: 2493-2501
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Intermediate
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 2839-2848
Chemical type: Peptide from protein
Sequence: SSLINGVVRL
Position: 2839-2848
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 2839-2848
Chemical type: Peptide from protein
Sequence: SSLINGVVRL
Position: 2839-2848
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Intermediate
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: C 18-37
Chemical type: Peptide from protein
Sequence: KRGVARVSPFGGLKRLPAGL
Position: 18-37
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E 754-770
Chemical type: Peptide from protein
Sequence: SQILIGTLLMWLGLNTK
Position: 754-770
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E 754-770
Chemical type: Peptide from protein
Sequence: SQILIGTLLMWLGLNTK
Position: 754-770
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E 754-770
Chemical type: Peptide from protein
Sequence: SQILIGTLLMWLGLNTK
Position: 754-770
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E 754-770
Chemical type: Peptide from protein
Sequence: SQILIGTLLMWLGLNTK
Position: 754-770
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS1 806-825
Chemical type: Peptide from protein
Sequence: ETRCGTGVFVYNDVEAWRDR
Position: 806-825
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-High
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS1 806-825
Chemical type: Peptide from protein
Sequence: ETRCGTGVFVYNDVEAWRDR
Position: 806-825
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS1 806-825
Chemical type: Peptide from protein
Sequence: ETRCGTGVFVYNDVEAWRDR
Position: 806-825
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Intermediate
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS1 806-825
Chemical type: Peptide from protein
Sequence: ETRCGTGVFVYNDVEAWRDR
Position: 806-825
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS1 806-825
Chemical type: Peptide from protein
Sequence: ETRCGTGVFVYNDVEAWRDR
Position: 806-825
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS1 835-855
Chemical type: Peptide from protein
Sequence: EENGVQLTVVVGSVKNPMWR
Position: 874-893
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Intermediate
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS1 940-958
Chemical type: Peptide from protein
Sequence: KHRAWNSFLVEDHGFGVFH
Position: 940-958
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS1 940-958
Chemical type: Peptide from protein
Sequence: KHRAWNSFLVEDHGFGVFH
Position: 940-958
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS1 940-958
Chemical type: Peptide from protein
Sequence: KHRAWNSFLVEDHGFGVFH
Position: 940-958
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-High
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS1 940-958
Chemical type: Peptide from protein
Sequence: KHRAWNSFLVEDHGFGVFH
Position: 940-958
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-High
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS1 940-958
Chemical type: Peptide from protein
Sequence: KHRAWNSFLVEDHGFGVFH
Position: 940-958
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3 1716-1734
Chemical type: Peptide from protein
Sequence: KTRLRTVILAPTRVVAAEM
Position: 1716-1734
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3 1791-1809
Chemical type: Peptide from protein
Sequence: FTDPSSIAARGYISTRVEM
Position: 1791-1809
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3 1791-1809
Chemical type: Peptide from protein
Sequence: FTDPSSIAARGYISTRVEM
Position: 1791-1809
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3 1791-1809
Chemical type: Peptide from protein
Sequence: FTDPSSIAARGYISTRVEM
Position: 1791-1809
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3 1791-1809
Chemical type: Peptide from protein
Sequence: FTDPSSIAARGYISTRVEM
Position: 1791-1809
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-High
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3 1791-1809
Chemical type: Peptide from protein
Sequence: FTDPSSIAARGYISTRVEM
Position: 1791-1809
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3 1791-1809
Chemical type: Peptide from protein
Sequence: FTDPSSIAARGYISTRVEM
Position: 1791-1809
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3 1791-1809
Chemical type: Peptide from protein
Sequence: FTDPSSIAARGYISTRVEM
Position: 1791-1809
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS3 1791-1809
Chemical type: Peptide from protein
Sequence: FTDPSSIAARGYISTRVEM
Position: 1791-1809
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4 2284-2303
Chemical type: Peptide from protein
Sequence: HLMGRREEGATIGFSMDIDL
Position: 2284-2303
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4 2092-2116
Chemical type: Peptide from protein
Sequence: TIGFSMDIDLRPASAWAIYA
Position: 2294-2313
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 2503-2520
Chemical type: Peptide from protein
Sequence: GASLIYTVTRNAGLVKRR
Position: 2503-2520
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 2850-2868
Chemical type: Peptide from protein
Sequence: SKPWDVVTGVTGIAMTDTT
Position: 2850-2868
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-High
Host:  6-8 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 2850-2868
Chemical type: Peptide from protein
Sequence: SKPWDVVTGVTGIAMTDTT
Position: 2850-2868
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS5 2850-2868
Chemical type: Peptide from protein
Sequence: SKPWDVVTGVTGIAMTDTT
Position: 2850-2868
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS2 1410-1428
Chemical type: Peptide from protein
Sequence: LIVSYVVSGKSVDMYIERA
Position: 1410-1428
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS2 1410-1428
Chemical type: Peptide from protein
Sequence: LIVSYVVSGKSVDMYIERA
Position: 1410-1428
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Negative
Host:  6-8 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS2 1410-1428
Chemical type: Peptide from protein
Sequence: LIVSYVVSGKSVDMYIERA
Position: 1410-1428
Source molecule (GenBank): AMA12084.1
Assay category: TCell
Result: Positive-Low
Host:  6-8 weeks
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo