Reference: Daniel J Browne; Pauline Crooks; Corey Smith; Denise L Doolan. "Differential reactivity of SARS-CoV-2 S-protein T-cell epitopes in vaccinated versus naturally infected individuals." Clinical & translational immunology (2025).

Epitope: 9
Chemical type: Peptide from protein
Sequence: NTQEVFAQV
Position: 777-785
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 9
Chemical type: Peptide from protein
Sequence: NTQEVFAQV
Position: 777-785
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 9
Chemical type: Peptide from protein
Sequence: NTQEVFAQV
Position: 777-785
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 13
Chemical type: Peptide from protein
Sequence: DISGINASV
Position: 1168-1176
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 13
Chemical type: Peptide from protein
Sequence: DISGINASV
Position: 1168-1176
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 13
Chemical type: Peptide from protein
Sequence: DISGINASV
Position: 1168-1176
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 18
Chemical type: Peptide from protein
Sequence: RLDKVEAEV
Position: 983-991
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 18
Chemical type: Peptide from protein
Sequence: RLDKVEAEV
Position: 983-991
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 18
Chemical type: Peptide from protein
Sequence: RLDKVEAEV
Position: 983-991
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 24
Chemical type: Peptide from protein
Sequence: HVSGTNGTK
Position: 69-77
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 24
Chemical type: Peptide from protein
Sequence: HVSGTNGTK
Position: 69-77
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 24
Chemical type: Peptide from protein
Sequence: HVSGTNGTK
Position: 69-77
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 33
Chemical type: Peptide from protein
Sequence: GVYYHKNNK
Position: 142-150
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 33
Chemical type: Peptide from protein
Sequence: GVYYHKNNK
Position: 142-150
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 33
Chemical type: Peptide from protein
Sequence: GVYYHKNNK
Position: 142-150
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 39
Chemical type: Peptide from protein
Sequence: RLFRKSNLK
Position: 454-462
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 39
Chemical type: Peptide from protein
Sequence: RLFRKSNLK
Position: 454-462
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 39
Chemical type: Peptide from protein
Sequence: RLFRKSNLK
Position: 454-462
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 42
Chemical type: Peptide from protein
Sequence: QTNSPRRAR
Position: 677-685
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 42
Chemical type: Peptide from protein
Sequence: QTNSPRRAR
Position: 677-685
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 42
Chemical type: Peptide from protein
Sequence: QTNSPRRAR
Position: 677-685
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 49
Chemical type: Peptide from protein
Sequence: VYSSANNCTF
Position: 159-168
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 49
Chemical type: Peptide from protein
Sequence: VYSSANNCTF
Position: 159-168
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 49
Chemical type: Peptide from protein
Sequence: VYSSANNCTF
Position: 159-168
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 59
Chemical type: Peptide from protein
Sequence: AYSNNSIAI
Position: 706-714
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 59
Chemical type: Peptide from protein
Sequence: AYSNNSIAI
Position: 706-714
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 59
Chemical type: Peptide from protein
Sequence: AYSNNSIAI
Position: 706-714
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 60
Chemical type: Peptide from protein
Sequence: QYIKWPWYI
Position: 1208-1216
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 60
Chemical type: Peptide from protein
Sequence: QYIKWPWYI
Position: 1208-1216
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 60
Chemical type: Peptide from protein
Sequence: QYIKWPWYI
Position: 1208-1216
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 70
Chemical type: Peptide from protein
Sequence: QPYRVVVLSF
Position: 506-515
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 70
Chemical type: Peptide from protein
Sequence: QPYRVVVLSF
Position: 506-515
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 70
Chemical type: Peptide from protein
Sequence: QPYRVVVLSF
Position: 506-515
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 91
Chemical type: Peptide from protein
Sequence: QPTESIVRF
Position: 321-329
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 91
Chemical type: Peptide from protein
Sequence: QPTESIVRF
Position: 321-329
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 91
Chemical type: Peptide from protein
Sequence: QPTESIVRF
Position: 321-329
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 103
Chemical type: Peptide from protein
Sequence: SEPVLKGVKL
Position: 1261-1270
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 103
Chemical type: Peptide from protein
Sequence: SEPVLKGVKL
Position: 1261-1270
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 103
Chemical type: Peptide from protein
Sequence: SEPVLKGVKL
Position: 1261-1270
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 111
Chemical type: Peptide from protein
Sequence: YPDKVFRSSV
Position: 38-47
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 111
Chemical type: Peptide from protein
Sequence: YPDKVFRSSV
Position: 38-47
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 111
Chemical type: Peptide from protein
Sequence: YPDKVFRSSV
Position: 38-47
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 125
Chemical type: Peptide from protein
Sequence: FNATRFASV
Position: 342-350
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 125
Chemical type: Peptide from protein
Sequence: FNATRFASV
Position: 342-350
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 125
Chemical type: Peptide from protein
Sequence: FNATRFASV
Position: 342-350
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 127
Chemical type: Peptide from protein
Sequence: YRLFRKSNL
Position: 453-461
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 127
Chemical type: Peptide from protein
Sequence: YRLFRKSNL
Position: 453-461
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 127
Chemical type: Peptide from protein
Sequence: YRLFRKSNL
Position: 453-461
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 143
Chemical type: Peptide from protein
Sequence: CVADYSVLY
Position: 361-369
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 143
Chemical type: Peptide from protein
Sequence: CVADYSVLY
Position: 361-369
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 143
Chemical type: Peptide from protein
Sequence: CVADYSVLY
Position: 361-369
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 155
Chemical type: Peptide from protein
Sequence: CEFQFCNDPFLGVYY
Position: 131-145
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 155
Chemical type: Peptide from protein
Sequence: CEFQFCNDPFLGVYY
Position: 131-145
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 155
Chemical type: Peptide from protein
Sequence: CEFQFCNDPFLGVYY
Position: 131-145
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 157
Chemical type: Peptide from protein
Sequence: NLVRDLPQGFSALEP
Position: 211-225
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 157
Chemical type: Peptide from protein
Sequence: NLVRDLPQGFSALEP
Position: 211-225
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 157
Chemical type: Peptide from protein
Sequence: NLVRDLPQGFSALEP
Position: 211-225
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo