Reference: Magdalini Moutaftsi; Bjoern Peters; Valerie Pasquetto; David C Tscharke; John Sidney; Huynh-Hoa Bui; Howard Grey; Alessandro Sette. "A consensus epitope prediction approach identifies the breadth of murine T(CD8+)-cell responses to vaccinia virus." Nature biotechnology (2006).

Epitope: C4L 125-132
Chemical type: Peptide from protein
Sequence: LNFRFENV
Position: 125-132
Source molecule (GenBank): P17370.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: C4L 125-132
Chemical type: Peptide from protein
Sequence: LNFRFENV
Position: 125-132
Source molecule (GenBank): P17370.1
Assay category: MhcBinding
Result: Negative

Epitope: C4L 125-132
Chemical type: Peptide from protein
Sequence: LNFRFENV
Position: 125-132
Source molecule (GenBank): P17370.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 0.84

Epitope: A26L 257-264
Chemical type: Peptide from protein
Sequence: SIYQYVRL
Position: 257-264
Source molecule (GenBank): P24759.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A26L 257-264
Chemical type: Peptide from protein
Sequence: SIYQYVRL
Position: 257-264
Source molecule (GenBank): P24759.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 8.2

Epitope: A26L 257-264
Chemical type: Peptide from protein
Sequence: SIYQYVRL
Position: 257-264
Source molecule (GenBank): P24759.1
Assay category: MhcBinding
Result: Negative

Epitope: A10L 265-272
Chemical type: Peptide from protein
Sequence: FIYTYDRV
Position: 265-272
Source molecule (GenBank): P16715.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A10L 265-272
Chemical type: Peptide from protein
Sequence: FIYTYDRV
Position: 265-272
Source molecule (GenBank): P16715.1
Assay category: MhcBinding
Result: Negative

Epitope: A10L 265-272
Chemical type: Peptide from protein
Sequence: FIYTYDRV
Position: 265-272
Source molecule (GenBank): P16715.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 13.0

Epitope: K ORF B 27-34
Chemical type: Peptide from protein
Sequence: IPLKYIVL
Position: 48-55
Source molecule (GenBank): AAW23428.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: K ORF B 27-34
Chemical type: Peptide from protein
Sequence: IPLKYIVL
Position: 48-55
Source molecule (GenBank): AAW23428.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 490.0

Epitope: K ORF B 27-34
Chemical type: Peptide from protein
Sequence: IPLKYIVL
Position: 48-55
Source molecule (GenBank): AAW23428.1
Assay category: MhcBinding
Result: Negative

Epitope: N2L  60-68
Chemical type: Peptide from protein
Sequence: FLMMNKDEL
Position: 60-68
Source molecule (GenBank): P14357.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: N2L  60-68
Chemical type: Peptide from protein
Sequence: FLMMNKDEL
Position: 60-68
Source molecule (GenBank): P14357.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 22765.0

Epitope: N2L  60-68
Chemical type: Peptide from protein
Sequence: FLMMNKDEL
Position: 60-68
Source molecule (GenBank): P14357.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 6.6

Epitope: D1R 578-586
Chemical type: Peptide from protein
Sequence: SMYCSKTFL
Position: 578-586
Source molecule (GenBank): P04298.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D1R 578-586
Chemical type: Peptide from protein
Sequence: SMYCSKTFL
Position: 578-586
Source molecule (GenBank): P04298.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D1R 578-586
Chemical type: Peptide from protein
Sequence: SMYCSKTFL
Position: 578-586
Source molecule (GenBank): P04298.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 15.0

Epitope: D1R 578-586
Chemical type: Peptide from protein
Sequence: SMYCSKTFL
Position: 578-586
Source molecule (GenBank): P04298.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 65.0

Epitope: D13L 118-126
Chemical type: Peptide from protein
Sequence: NCINNTIAL
Position: 118-126
Source molecule (GenBank): P68440.1
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D13L 118-126
Chemical type: Peptide from protein
Sequence: NCINNTIAL
Position: 118-126
Source molecule (GenBank): P68440.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 2220.0

Epitope: D13L 118-126
Chemical type: Peptide from protein
Sequence: NCINNTIAL
Position: 118-126
Source molecule (GenBank): P68440.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 17736.0