Reference: Neha Sangewar; Suryakant D Waghela; Jianxiu Yao; Huldah Sang; Jocelyn Bray; Waithaka Mwangi. "Novel Potent IFN-γ-Inducing CD8<sup>+</sup> T Cell Epitopes Conserved among Diverse Bovine Viral Diarrhea Virus Strains." Journal of immunology (Baltimore, Md. : 1950) (2021).

Epitope: Peptide-2
Chemical type: Peptide from protein
Sequence: VQYTARGQL
Position: 1546-1554
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-2
Chemical type: Peptide from protein
Sequence: VQYTARGQL
Position: 1546-1554
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-7
Chemical type: Peptide from protein
Sequence: GEYQYWFDL
Position: 1014-1022
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-7
Chemical type: Peptide from protein
Sequence: GEYQYWFDL
Position: 1014-1022
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-15
Chemical type: Peptide from protein
Sequence: FAPETASVL
Position: 565-573
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-15
Chemical type: Peptide from protein
Sequence: FAPETASVL
Position: 565-573
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-20
Chemical type: Peptide from protein
Sequence: NMMDKLTAF
Position: 1606-1614
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-20
Chemical type: Peptide from protein
Sequence: NMMDKLTAF
Position: 1606-1614
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-36
Chemical type: Peptide from protein
Sequence: RQLGILGKK
Position: 476-484
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-36
Chemical type: Peptide from protein
Sequence: RQLGILGKK
Position: 476-484
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4B-2585-2593
Chemical type: Peptide from protein
Sequence: SEQKRTLLM
Position: 2585-2593
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Positive
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4B-2585-2593
Chemical type: Peptide from protein
Sequence: SEQKRTLLM
Position: 2585-2593
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Positive
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4B-2664-2672
Chemical type: Peptide from protein
Sequence: IMFEAFELL
Position: 2664-2672
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Positive
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4B-2664-2672
Chemical type: Peptide from protein
Sequence: IMFEAFELL
Position: 2664-2672
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Positive
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-50
Chemical type: Peptide from protein
Sequence: IPNWRPLTF
Position: 1247-1255
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-50
Chemical type: Peptide from protein
Sequence: IPNWRPLTF
Position: 1247-1255
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-75
Chemical type: Peptide from protein
Sequence: YIPDKGYTL
Position: 3840-3848
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-75
Chemical type: Peptide from protein
Sequence: YIPDKGYTL
Position: 3840-3848
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-80
Chemical type: Peptide from protein
Sequence: KVLKWVHNK
Position: 150-158
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-80
Chemical type: Peptide from protein
Sequence: KVLKWVHNK
Position: 150-158
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-124
Chemical type: Peptide from protein
Sequence: TPSDERIRL
Position: 2720-2728
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-124
Chemical type: Peptide from protein
Sequence: TPSDERIRL
Position: 2720-2728
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-135
Chemical type: Peptide from protein
Sequence: DSKLYHIYV
Position: 125-133
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-135
Chemical type: Peptide from protein
Sequence: DSKLYHIYV
Position: 125-133
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-148
Chemical type: Peptide from protein
Sequence: DTKSFHEAI
Position: 3340-3348
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-148
Chemical type: Peptide from protein
Sequence: DTKSFHEAI
Position: 3340-3348
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-150
Chemical type: Peptide from protein
Sequence: KIHPYEAYL
Position: 3244-3252
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-150
Chemical type: Peptide from protein
Sequence: KIHPYEAYL
Position: 3244-3252
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-164
Chemical type: Peptide from protein
Sequence: YLERVDLSF
Position: 1143-1151
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-164
Chemical type: Peptide from protein
Sequence: YLERVDLSF
Position: 1143-1151
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4B-2555-2563
Chemical type: Peptide from protein
Sequence: MEILSQNPV
Position: 2555-2563
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Positive
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4B-2555-2563
Chemical type: Peptide from protein
Sequence: MEILSQNPV
Position: 2555-2563
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Positive
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E-rNS3-63-371
Chemical type: Peptide from protein
Sequence: LMNKTQANL
Position: 363-371
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Positive
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: E-rNS3-63-371
Chemical type: Peptide from protein
Sequence: LMNKTQANL
Position: 363-371
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Positive
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4A-2291-2299
Chemical type: Peptide from protein
Sequence: ALSKRHVPM
Position: 2291-2299
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Positive
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NS4A-2291-2299
Chemical type: Peptide from protein
Sequence: ALSKRHVPM
Position: 2291-2299
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Positive
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-192
Chemical type: Peptide from protein
Sequence: AMFQRGVNR
Position: 289-297
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Peptide-192
Chemical type: Peptide from protein
Sequence: AMFQRGVNR
Position: 289-297
Source molecule (GenBank): ADX31695.1
Assay category: TCell
Result: Negative
Host: M 7-8 months
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo