Reference: Marios Koutsakos; Patricia T Illing; Thi H O Nguyen; Nicole A Mifsud; Jeremy Chase Crawford; Simone Rizzetto; Auda A Eltahla; E Bridie Clemens; Sneha Sant; Brendon Y Chua; Chinn Yi Wong; E Kaitlynn Allen; Don Teng; Pradyot Dash; David F Boyd; Ludivine Grzelak; Weiguang Zeng; Aeron C Hurt; Ian Barr; Steve Rockman; David C Jackson; Tom C Kotsimbos; Allen C Cheng; Michael Richards; Glen P Westall; Thomas Loudovaris; Stuart I Mannering; Michael Elliott; Stuart G Tangye; Linda M Wakim; Jamie Rossjohn; Dhanasekaran Vijaykrishna; Fabio Luciani; Paul G Thomas; Stephanie Gras; Anthony W Purcell; Katherine Kedzierska. "Human CD8<sup>+</sup> T cell cross-reactivity across influenza A, B and C viruses." Nature immunology (2019).

Epitope: PB1 540–548
Chemical type: Peptide from protein
Sequence: GPATAQMAL
Position: 540-548
Source molecule (GenBank): AAA43637.1
Assay category: TCell
Result: Negative
Host:  22-71 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: PB1 490–497
Chemical type: Peptide from protein
Sequence: FEFTSFFY
Position: 490-497
Source molecule (GenBank): AAA43637.1
Assay category: TCell
Result: Negative
Host:  22-71 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: PB1 490–497
Chemical type: Peptide from protein
Sequence: FEFTSFFY
Position: 490-497
Source molecule (GenBank): AAA43637.1
Assay category: TCell
Result: Negative
Host:  22-71 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HA 486
Chemical type: Peptide from protein
Sequence: KMLGPSAVEI
Position: 483-492
Source molecule (GenBank): 1464282948
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 486
Chemical type: Peptide from protein
Sequence: KMLGPSAVEI
Position: 483-492
Source molecule (GenBank): 1464282948
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 486
Chemical type: Peptide from protein
Sequence: KMLGPSAVEI
Position: 483-492
Source molecule (GenBank): 1464282948
Assay category: MhcLigandElution
Result: Positive

Epitope: HA 543
Chemical type: Peptide from protein
Sequence: GLDNHTILL
Position: 540-548
Source molecule (GenBank): 1464282948
Assay category: TCell
Result: Positive
Host:  42-65 years
Cell type: T cell CD8+
Tissue: lung
Culture conditions: Direct Ex Vivo

Epitope: HA 543
Chemical type: Peptide from protein
Sequence: GLDNHTILL
Position: 540-548
Source molecule (GenBank): 1464282948
Assay category: TCell
Result: Positive
Host:  22-71 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: HA 543
Chemical type: Peptide from protein
Sequence: GLDNHTILL
Position: 540-548
Source molecule (GenBank): 1464282948
Assay category: TCell
Result: Positive
Host:  22-71 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: HA 543
Chemical type: Peptide from protein
Sequence: GLDNHTILL
Position: 540-548
Source molecule (GenBank): 1464282948
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: bronchial aveolar lavage (BAL)
Culture conditions: Direct Ex Vivo

Epitope: HA 543
Chemical type: Peptide from protein
Sequence: GLDNHTILL
Position: 540-548
Source molecule (GenBank): 1464282948
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: bronchial aveolar lavage (BAL)
Culture conditions: Direct Ex Vivo

Epitope: HA 543
Chemical type: Peptide from protein
Sequence: GLDNHTILL
Position: 540-548
Source molecule (GenBank): 1464282948
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 543
Chemical type: Peptide from protein
Sequence: GLDNHTILL
Position: 540-548
Source molecule (GenBank): 1464282948
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 543
Chemical type: Peptide from protein
Sequence: GLDNHTILL
Position: 540-548
Source molecule (GenBank): 1464282948
Assay category: TCell
Result: Positive
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: HA 543
Chemical type: Peptide from protein
Sequence: GLDNHTILL
Position: 540-548
Source molecule (GenBank): 1464282948
Assay category: TCell
Result: Negative
Host:  13-77 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HA 543
Chemical type: Peptide from protein
Sequence: GLDNHTILL
Position: 540-548
Source molecule (GenBank): 1464282948
Assay category: TCell
Result: Positive
Host:  6-33 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HA 543
Chemical type: Peptide from protein
Sequence: GLDNHTILL
Position: 540-548
Source molecule (GenBank): 1464282948
Assay category: TCell
Result: Positive
Host:  22-71 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: HA 543
Chemical type: Peptide from protein
Sequence: GLDNHTILL
Position: 540-548
Source molecule (GenBank): 1464282948
Assay category: MhcLigandElution
Result: Positive

Epitope: M1 132
Chemical type: Peptide from protein
Sequence: YLNPGNYSM
Position: 132-140
Source molecule (GenBank): 1444411481
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M1 132
Chemical type: Peptide from protein
Sequence: YLNPGNYSM
Position: 132-140
Source molecule (GenBank): 1444411481
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M1 132
Chemical type: Peptide from protein
Sequence: YLNPGNYSM
Position: 132-140
Source molecule (GenBank): 1444411481
Assay category: TCell
Result: Positive
Host:  22-71 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: M1 132
Chemical type: Peptide from protein
Sequence: YLNPGNYSM
Position: 132-140
Source molecule (GenBank): 1444411481
Assay category: TCell
Result: Positive
Host:  22-71 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: M1 132
Chemical type: Peptide from protein
Sequence: YLNPGNYSM
Position: 132-140
Source molecule (GenBank): 1444411481
Assay category: MhcLigandElution
Result: Positive

Epitope: M1 200
Chemical type: Peptide from protein
Sequence: KLAEELQSNIGVL
Position: 117-129
Source molecule (GenBank): 814944606
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M1 200
Chemical type: Peptide from protein
Sequence: KLAEELQSNIGVL
Position: 117-129
Source molecule (GenBank): 814944606
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: M1 200
Chemical type: Peptide from protein
Sequence: KLAEELQSNIGVL
Position: 117-129
Source molecule (GenBank): 814944606
Assay category: MhcLigandElution
Result: Positive

Epitope: NA 454
Chemical type: Peptide from protein
Sequence: LLWDTVTGVNM
Position: 454-464
Source molecule (GenBank): 1464283005
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NA 454
Chemical type: Peptide from protein
Sequence: LLWDTVTGVNM
Position: 454-464
Source molecule (GenBank): 1464283005
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NA 454
Chemical type: Peptide from protein
Sequence: LLWDTVTGVNM
Position: 454-464
Source molecule (GenBank): 1464283005
Assay category: MhcLigandElution
Result: Positive

Epitope: NP 78
Chemical type: Peptide from protein
Sequence: SVYNMVVKL
Position: 78-86
Source molecule (GenBank): 1464282937
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 78
Chemical type: Peptide from protein
Sequence: SVYNMVVKL
Position: 78-86
Source molecule (GenBank): 1464282937
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 78
Chemical type: Peptide from protein
Sequence: SVYNMVVKL
Position: 78-86
Source molecule (GenBank): 1464282937
Assay category: MhcLigandElution
Result: Positive

Epitope: NP 85
Chemical type: Peptide from protein
Sequence: KLGEFYNQMM
Position: 85-94
Source molecule (GenBank): 1464282937
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 85
Chemical type: Peptide from protein
Sequence: KLGEFYNQMM
Position: 85-94
Source molecule (GenBank): 1464282937
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 85
Chemical type: Peptide from protein
Sequence: KLGEFYNQMM
Position: 85-94
Source molecule (GenBank): 1464282937
Assay category: MhcLigandElution
Result: Positive

Epitope: NP 85
Chemical type: Peptide from protein
Sequence: KLGEFYNQMMV
Position: 85-95
Source molecule (GenBank): 1464282937
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 85
Chemical type: Peptide from protein
Sequence: KLGEFYNQMMV
Position: 85-95
Source molecule (GenBank): 1464282937
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 85
Chemical type: Peptide from protein
Sequence: KLGEFYNQMMV
Position: 85-95
Source molecule (GenBank): 1464282937
Assay category: MhcLigandElution
Result: Positive

Epitope: NP 289
Chemical type: Peptide from protein
Sequence: SAPQQKALVD
Position: 289-298
Source molecule (GenBank): 1464282937
Assay category: MhcLigandElution
Result: Positive

Epitope: NP 307
Chemical type: Peptide from protein
Sequence: GIADIEDLTLLAR
Position: 307-319
Source molecule (GenBank): 1464282937
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 307
Chemical type: Peptide from protein
Sequence: GIADIEDLTLLAR
Position: 307-319
Source molecule (GenBank): 1464282937
Assay category: TCell
Result: Negative
Host:  6-12 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NP 307
Chemical type: Peptide from protein
Sequence: GIADIEDLTLLAR
Position: 307-319
Source molecule (GenBank): 1464282937
Assay category: MhcLigandElution
Result: Positive

Epitope: PA 617
Chemical type: Peptide from protein
Sequence: IGTQEGKLVK
Position: 617-626
Source molecule (GenBank): 1464282954
Assay category: MhcLigandElution
Result: Positive

Epitope: HA 91
Chemical type: Peptide from protein
Sequence: IPSARVSIL
Position: 91-99
Source molecule (GenBank): 1464282948
Assay category: MhcLigandElution
Result: Positive

Epitope: BM2 43
Chemical type: Peptide from protein
Sequence: GPNKETINREVSILRHSYQK
Position: 43-62
Source molecule (GenBank): 1464282943
Assay category: MhcLigandElution
Result: Positive

Epitope: BM2 51
Chemical type: Peptide from protein
Sequence: REVSILRHSYQ
Position: 51-61
Source molecule (GenBank): 1464282943
Assay category: MhcLigandElution
Result: Positive

Epitope: BM2 74
Chemical type: Peptide from protein
Sequence: LSDNMEVLSDHIIIE
Position: 74-88
Source molecule (GenBank): 549408925
Assay category: MhcLigandElution
Result: Positive

Epitope: BM2 83
Chemical type: Peptide from protein
Sequence: DHIIIEGLSAEEI
Position: 83-95
Source molecule (GenBank): 1464282943
Assay category: MhcLigandElution
Result: Positive

Epitope: BM2 86
Chemical type: Peptide from protein
Sequence: IIEGLSAEEIIKMG
Position: 86-99
Source molecule (GenBank): 1464282943
Assay category: MhcLigandElution
Result: Positive

Epitope: HA 15
Chemical type: Peptide from protein
Sequence: ADRICTGITSSNSPH
Position: 15-29
Source molecule (GenBank): 1464282948
Assay category: MhcLigandElution
Result: Positive

Epitope: HA 50
Chemical type: Peptide from protein
Sequence: TPTKSHFANLKGTETR
Position: 50-65
Source molecule (GenBank): 1464282948
Assay category: MhcLigandElution
Result: Positive

Epitope: HA 114
Chemical type: Peptide from protein
Sequence: HDRTKIRQLPNLLR
Position: 114-127
Source molecule (GenBank): 1464282948
Assay category: MhcLigandElution
Result: Positive

Epitope: HA 252
Chemical type: Peptide from protein
Sequence: DGGLPQSGRIVVD
Position: 249-261
Source molecule (GenBank): 1464282948
Assay category: MhcLigandElution
Result: Positive

Epitope: HA 266
Chemical type: Peptide from protein
Sequence: MVQKSGKTGTITYQRGILLPQ
Position: 263-283
Source molecule (GenBank): 1464282948
Assay category: MhcLigandElution
Result: Positive

Epitope: HA 273
Chemical type: Peptide from protein
Sequence: TGTITYQRGILLPQK
Position: 270-284
Source molecule (GenBank): 1464282948
Assay category: MhcLigandElution
Result: Positive

Epitope: HA 391
Chemical type: Peptide from protein
Sequence: AHGVAVAADLKSTQEA
Position: 388-403
Source molecule (GenBank): 1464282948
Assay category: MhcLigandElution
Result: Positive

Epitope: HA 410
Chemical type: Peptide from protein
Sequence: ITKNLNSLSELEVK
Position: 407-420
Source molecule (GenBank): 1464282948
Assay category: MhcLigandElution
Result: Positive

Epitope: HA 412
Chemical type: Peptide from protein
Sequence: KNLNSLSELEVKN
Position: 409-421
Source molecule (GenBank): 1464282948
Assay category: MhcLigandElution
Result: Positive

Epitope: HA 413
Chemical type: Peptide from protein
Sequence: NLNSLSELEVKNLQR
Position: 410-424
Source molecule (GenBank): 1464282948
Assay category: MhcLigandElution
Result: Positive

Epitope: HA 419
Chemical type: Peptide from protein
Sequence: ELEVKNLQRLSGAMDE
Position: 416-431
Source molecule (GenBank): 1464282948
Assay category: MhcLigandElution
Result: Positive

Epitope: HA 448
Chemical type: Peptide from protein
Sequence: DLRADTISSQIELAVLLSNEGIINSEDE
Position: 445-472
Source molecule (GenBank): 1464282948
Assay category: MhcLigandElution
Result: Positive

Epitope: HA 460
Chemical type: Peptide from protein
Sequence: LAVLLSNEGIINSEDE
Position: 457-472
Source molecule (GenBank): 1464282948
Assay category: MhcLigandElution
Result: Positive

Epitope: HA 460
Chemical type: Peptide from protein
Sequence: LAVLLSNEGIINSEDEHL
Position: 457-474
Source molecule (GenBank): 1464282948
Assay category: MhcLigandElution
Result: Positive

Epitope: HA 464
Chemical type: Peptide from protein
Sequence: LSNEGIINSEDEH
Position: 461-473
Source molecule (GenBank): 1464282948
Assay category: MhcLigandElution
Result: Positive

Epitope: M1 205
Chemical type: Peptide from protein
Sequence: LQSNIGVLRSLGAS
Position: 205-218
Source molecule (GenBank): 1142419281
Assay category: MhcLigandElution
Result: Positive

Epitope: M1 208
Chemical type: Peptide from protein
Sequence: NIGVLRSLGAS
Position: 208-218
Source molecule (GenBank): 1142419281
Assay category: MhcLigandElution
Result: Positive

Epitope: M1 208
Chemical type: Peptide from protein
Sequence: NIGVLRSLGASQ
Position: 208-219
Source molecule (GenBank): 1444411481
Assay category: MhcLigandElution
Result: Positive

Epitope: M1 220
Chemical type: Peptide from protein
Sequence: KNGEGIAKDVMEVLKQSSMGNS
Position: 220-241
Source molecule (GenBank): 1444411481
Assay category: MhcLigandElution
Result: Positive

Epitope: M1 225
Chemical type: Peptide from protein
Sequence: IAKDVMEVLKQSSMGNSA
Position: 225-242
Source molecule (GenBank): 1444411481
Assay category: MhcLigandElution
Result: Positive

Epitope: NA 184
Chemical type: Peptide from protein
Sequence: DGKEWTYIGVDGPDNNAL
Position: 184-201
Source molecule (GenBank): 324033764
Assay category: MhcLigandElution
Result: Positive

Epitope: NP 17
Chemical type: Peptide from protein
Sequence: APEEITSGTSGTTR
Position: 17-30
Source molecule (GenBank): 225676901
Assay category: MhcLigandElution
Result: Positive

Epitope: NP 105
Chemical type: Peptide from protein
Sequence: RNLIQNAHAVER
Position: 105-116
Source molecule (GenBank): 1464282937
Assay category: MhcLigandElution
Result: Positive

Epitope: NP 241
Chemical type: Peptide from protein
Sequence: GVAIKGGGTLVAEAI
Position: 241-255
Source molecule (GenBank): 1464282937
Assay category: MhcLigandElution
Result: Positive

Epitope: NP 421
Chemical type: Peptide from protein
Sequence: VPAKEQVEGMGAALMS
Position: 421-436
Source molecule (GenBank): 1464282937
Assay category: MhcLigandElution
Result: Positive