Reference: Stephen R Walsh; Jacqueline Gillis; Björn Peters; Bianca R Mothé; John Sidney; Alessandro Sette; R Paul Johnson. "Diverse recognition of conserved orthopoxvirus CD8+ T cell epitopes in vaccinated rhesus macaques." Vaccine (2009).

Epitope: 7P
Chemical type: Peptide from protein
Sequence: WTPVTEDDYKF
Position: 277-287
Source molecule (GenBank): AAO89289.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 7P
Chemical type: Peptide from protein
Sequence: WTPVTEDDYKF
Position: 277-287
Source molecule (GenBank): AAO89289.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.1

Epitope: 8P
Chemical type: Peptide from protein
Sequence: YTPNNTTTI
Position: 138-146
Source molecule (GenBank): AAO89290.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 8P
Chemical type: Peptide from protein
Sequence: YTPNNTTTI
Position: 138-146
Source molecule (GenBank): AAO89290.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 31.0

Epitope: 15P
Chemical type: Peptide from protein
Sequence: IIPFIAYFV
Position: 427-435
Source molecule (GenBank): AAO89309.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 15P
Chemical type: Peptide from protein
Sequence: IIPFIAYFV
Position: 427-435
Source molecule (GenBank): AAO89309.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 1936.0

Epitope: 32P
Chemical type: Peptide from protein
Sequence: YSPMETTAEF
Position: 119-128
Source molecule (GenBank): AAO89328.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 32P
Chemical type: Peptide from protein
Sequence: YSPMETTAEF
Position: 119-128
Source molecule (GenBank): AAO89328.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 1505.0

Epitope: 33P
Chemical type: Peptide from protein
Sequence: PTPKKMNIV
Position: 27-35
Source molecule (GenBank): AAO89329.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 33P
Chemical type: Peptide from protein
Sequence: PTPKKMNIV
Position: 27-35
Source molecule (GenBank): AAO89329.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 17.0

Epitope: 35P
Chemical type: Peptide from protein
Sequence: SSPPIPMSRL
Position: 73-82
Source molecule (GenBank): AAO89330.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 35P
Chemical type: Peptide from protein
Sequence: SSPPIPMSRL
Position: 73-82
Source molecule (GenBank): AAO89330.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 254.0

Epitope: 36P
Chemical type: Peptide from protein
Sequence: YSPKTSDYPL
Position: 93-102
Source molecule (GenBank): AAO89330.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 36P
Chemical type: Peptide from protein
Sequence: YSPKTSDYPL
Position: 93-102
Source molecule (GenBank): AAO89330.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 8896.0

Epitope: 43P
Chemical type: Peptide from protein
Sequence: FSPFKLNLI
Position: 170-178
Source molecule (GenBank): AAO89340.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 43P
Chemical type: Peptide from protein
Sequence: FSPFKLNLI
Position: 170-178
Source molecule (GenBank): AAO89340.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 0.15

Epitope: 67P
Chemical type: Peptide from protein
Sequence: YTPQIYTYS
Position: 128-136
Source molecule (GenBank): AAO89359.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 67P
Chemical type: Peptide from protein
Sequence: YTPQIYTYS
Position: 128-136
Source molecule (GenBank): AAO89359.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.9

Epitope: 78P
Chemical type: Peptide from protein
Sequence: YTPIGDNKAL
Position: 163-172
Source molecule (GenBank): AAO89370.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 78P
Chemical type: Peptide from protein
Sequence: YTPIGDNKAL
Position: 163-172
Source molecule (GenBank): AAO89370.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 0.9

Epitope: 87P
Chemical type: Peptide from protein
Sequence: ISPDEIVIKF
Position: 155-164
Source molecule (GenBank): AAO89383.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 87P
Chemical type: Peptide from protein
Sequence: ISPDEIVIKF
Position: 155-164
Source molecule (GenBank): AAO89383.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 0.24

Epitope: 93P
Chemical type: Peptide from protein
Sequence: GTPTVAHIY
Position: 41-49
Source molecule (GenBank): AAO89386.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 93P
Chemical type: Peptide from protein
Sequence: GTPTVAHIY
Position: 41-49
Source molecule (GenBank): AAO89386.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 0.69

Epitope: 123P
Chemical type: Peptide from protein
Sequence: SSPEEISSQL
Position: 55-64
Source molecule (GenBank): AAO89409.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 123P
Chemical type: Peptide from protein
Sequence: SSPEEISSQL
Position: 55-64
Source molecule (GenBank): AAO89409.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 66.0

Epitope: 134P
Chemical type: Peptide from protein
Sequence: ATPRPANRI
Position: 236-244
Source molecule (GenBank): AAO89417.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 134P
Chemical type: Peptide from protein
Sequence: ATPRPANRI
Position: 236-244
Source molecule (GenBank): AAO89417.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 39.0

Epitope: 149P
Chemical type: Peptide from protein
Sequence: ITPMGVLTI
Position: 7-15
Source molecule (GenBank): AAO89437.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 149P
Chemical type: Peptide from protein
Sequence: ITPMGVLTI
Position: 7-15
Source molecule (GenBank): AAO89437.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 0.32

Epitope: 162P
Chemical type: Peptide from protein
Sequence: LTPIFSDLL
Position: 199-207
Source molecule (GenBank): AAO89452.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 162P
Chemical type: Peptide from protein
Sequence: LTPIFSDLL
Position: 199-207
Source molecule (GenBank): AAO89452.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 9.7

Epitope: 168P
Chemical type: Peptide from protein
Sequence: LSPQVIKSL
Position: 212-220
Source molecule (GenBank): AAO89459.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 168P
Chemical type: Peptide from protein
Sequence: LSPQVIKSL
Position: 212-220
Source molecule (GenBank): AAO89459.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 2.2

Epitope: 174P
Chemical type: Peptide from protein
Sequence: TTPFYCGII
Position: 278-286
Source molecule (GenBank): AAO89465.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 174P
Chemical type: Peptide from protein
Sequence: TTPFYCGII
Position: 278-286
Source molecule (GenBank): AAO89465.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 47.0

Epitope: 176P
Chemical type: Peptide from protein
Sequence: GTPTVAHIY
Position: 41-49
Source molecule (GenBank): YP_232989.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 176P
Chemical type: Peptide from protein
Sequence: GTPTVAHIY
Position: 41-49
Source molecule (GenBank): YP_232989.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.4

Epitope: 177P
Chemical type: Peptide from protein
Sequence: ITPWSSKVCL
Position: 199-208
Source molecule (GenBank): AAO89469.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 177P
Chemical type: Peptide from protein
Sequence: ITPWSSKVCL
Position: 199-208
Source molecule (GenBank): AAO89469.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 107.0

Epitope: 182P
Chemical type: Peptide from protein
Sequence: VSPTEMVDV
Position: 156-164
Source molecule (GenBank): AAO89474.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 182P
Chemical type: Peptide from protein
Sequence: VSPTEMVDV
Position: 156-164
Source molecule (GenBank): AAO89474.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 188.0

Epitope: 189P
Chemical type: Peptide from protein
Sequence: RTPLHKYLCKF
Position: 150-160
Source molecule (GenBank): AAO89482.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 189P
Chemical type: Peptide from protein
Sequence: RTPLHKYLCKF
Position: 150-160
Source molecule (GenBank): AAO89482.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 170.0

Epitope: 190P
Chemical type: Peptide from protein
Sequence: VVPNTLII
Position: 297-304
Source molecule (GenBank): AAO89482.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 190P
Chemical type: Peptide from protein
Sequence: VVPNTLII
Position: 297-304
Source molecule (GenBank): AAO89482.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 0.8

Epitope: 2NP
Chemical type: Peptide from protein
Sequence: KSDEIFALL
Position: 117-125
Source molecule (GenBank): AAO89427.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 2NP
Chemical type: Peptide from protein
Sequence: KSDEIFALL
Position: 117-125
Source molecule (GenBank): AAO89427.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 2.8

Epitope: 8NP
Chemical type: Peptide from protein
Sequence: FTTPLISFF
Position: 275-283
Source molecule (GenBank): AAO89380.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 8NP
Chemical type: Peptide from protein
Sequence: FTTPLISFF
Position: 275-283
Source molecule (GenBank): AAO89380.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 16.7

Epitope: 27NP
Chemical type: Peptide from protein
Sequence: LTSEIVNLL
Position: 975-983
Source molecule (GenBank): AAO89344.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 27NP
Chemical type: Peptide from protein
Sequence: LTSEIVNLL
Position: 975-983
Source molecule (GenBank): AAO89344.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 6.5

Epitope: 29NP
Chemical type: Peptide from protein
Sequence: ITSGNINYM
Position: 178-186
Source molecule (GenBank): AAO89473.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 29NP
Chemical type: Peptide from protein
Sequence: ITSGNINYM
Position: 178-186
Source molecule (GenBank): AAO89473.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 42.0

Epitope: 40NP
Chemical type: Peptide from protein
Sequence: MTLPSIFLI
Position: 192-200
Source molecule (GenBank): AAO89441.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 40NP
Chemical type: Peptide from protein
Sequence: MTLPSIFLI
Position: 192-200
Source molecule (GenBank): AAO89441.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 4.2

Epitope: 48NP
Chemical type: Peptide from protein
Sequence: GTYVCAFFM
Position: 99-107
Source molecule (GenBank): AAO89460.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 48NP
Chemical type: Peptide from protein
Sequence: GTYVCAFFM
Position: 99-107
Source molecule (GenBank): AAO89460.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 136.0

Epitope: 56NP
Chemical type: Peptide from protein
Sequence: RTRHFLSYI
Position: 137-145
Source molecule (GenBank): AAO89465.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 56NP
Chemical type: Peptide from protein
Sequence: RTRHFLSYI
Position: 137-145
Source molecule (GenBank): AAO89465.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 66.9

Epitope: 80NP
Chemical type: Peptide from protein
Sequence: RTIILVGYM
Position: 276-284
Source molecule (GenBank): AAO89330.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 80NP
Chemical type: Peptide from protein
Sequence: RTIILVGYM
Position: 276-284
Source molecule (GenBank): AAO89330.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 1672.7

Epitope: 82NP
Chemical type: Peptide from protein
Sequence: KTFAIIAIV
Position: 274-282
Source molecule (GenBank): AAO89392.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 82NP
Chemical type: Peptide from protein
Sequence: KTFAIIAIV
Position: 274-282
Source molecule (GenBank): AAO89392.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 2731.8

Epitope: 93NP
Chemical type: Peptide from protein
Sequence: MSILPVIFL
Position: 1-9
Source molecule (GenBank): AAO89476.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 93NP
Chemical type: Peptide from protein
Sequence: MSILPVIFL
Position: 1-9
Source molecule (GenBank): AAO89476.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 26.5

Epitope: 96NP
Chemical type: Peptide from protein
Sequence: CSYDMFNLL
Position: 254-262
Source molecule (GenBank): AAO89449.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 96NP
Chemical type: Peptide from protein
Sequence: CSYDMFNLL
Position: 254-262
Source molecule (GenBank): AAO89449.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 3.8

Epitope: 98NP
Chemical type: Peptide from protein
Sequence: ITMSAFLIV
Position: 49-57
Source molecule (GenBank): AAO89435.1
Assay category: TCell
Result: Negative
Host:  adult
Cell type: PBMC
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: 98NP
Chemical type: Peptide from protein
Sequence: ITMSAFLIV
Position: 49-57
Source molecule (GenBank): AAO89435.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 28.8