Reference: Janna Heide; Sophia Schulte; Matin Kohsar; Thomas Theo Brehm; Marissa Herrmann; Hendrik Karsten; Matthias Marget; Sven Peine; Alexandra M Johansson; Alessandro Sette; Marc Lütgehetmann; William W Kwok; John Sidney; Julian Schulze Zur Wiesch. "Broadly directed SARS-CoV-2-specific CD4+ T cell response includes frequently detected peptide specificities within the membrane and nucleoprotein in patients with acute and resolved COVID-19." PLoS pathogens (2021).

Epitope: Ncl_P5
Chemical type: Peptide from protein
Sequence: SDSTGSNQNGERSGA
Position: 21-35
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
MHC allele types: 57 419 316 317
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P14
Chemical type: Peptide from protein
Sequence: FPRGQGVPINTNSSP
Position: 66-80
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P16
Chemical type: Peptide from protein
Sequence: TNSSPDDQIGYYRRA
Position: 76-90
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
MHC allele types: 57 414 316 320
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P19
Chemical type: Peptide from protein
Sequence: TRRIRGGDGKMKDLS
Position: 91-105
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P27
Chemical type: Peptide from protein
Sequence: IWVATEGALNTPKDH
Position: 131-145
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P36
Chemical type: Peptide from protein
Sequence: SRGGSQASSRSSSRS
Position: 176-190
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
MHC allele types: 436 323
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P41
Chemical type: Peptide from protein
Sequence: SSKRTSPARMAGNGG
Position: 201-215
Source molecule (GenBank): QMT50097.1
Assay category: TCell
Result: Positive
MHC allele types: 437 818
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 840.0

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 40000.0

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 1141.0

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 12530.0

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 317.0

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 824.0

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 486.0

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 7139.0

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 85.0

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 40000.0

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 43.0

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 26925.0

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 9037.0

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 2446.0

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 6091.0

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 1162.0

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 2779.0

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 2487.0

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 22499.0

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 860.0

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 8458.0

Epitope: Ncl_P44
Chemical type: Peptide from protein
Sequence: DAALALLLLDRLNQL
Position: 216-230
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 2499.0

Epitope: Ncl_P46
Chemical type: Peptide from protein
Sequence: RLNQLESKMSGKGQQ
Position: 226-240
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P53
Chemical type: Peptide from protein
Sequence: KRTATKAYNVTQAFG
Position: 261-275
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P71
Chemical type: Peptide from protein
Sequence: ILLNKHIDAYKTFPP
Position: 351-365
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P71
Chemical type: Peptide from protein
Sequence: ILLNKHIDAYKTFPP
Position: 351-365
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P71
Chemical type: Peptide from protein
Sequence: ILLNKHIDAYKTFPP
Position: 351-365
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Ncl_P79
Chemical type: Peptide from protein
Sequence: TVTLLPAADLDDFSK
Position: 391-405
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Env_P10
Chemical type: Peptide from protein
Sequence: IVNVSLVKPSFYVYS
Position: 46-60
Source molecule (GenBank): P0DTC4.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Mem_P20
Chemical type: Peptide from protein
Sequence: FIASFRLFARTRSMW
Position: 96-110
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Mem_P20
Chemical type: Peptide from protein
Sequence: FIASFRLFARTRSMW
Position: 96-110
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Positive
Host:  25-78 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated