Reference: Chanchan Xiao; Zhiyao Ren; Bei Zhang; Lipeng Mao; Guodong Zhu; Lijuan Gao; Jun Su; Jiezhou Ye; Ze Long; Yue Zhu; Pengfei Chen; Xiangmeng Su; Tong Zhou; Yanhao Huang; Xiongfei Chen; Chaojun Xie; Jun Yuan; Yutian Hu; Jingshan Zheng; Zhigang Wang; Jianrong Lou; Xiang Yang; Zhiqiang Kuang; Hongyi Zhang; Pengcheng Wang; Xiaofeng Liang; Oscar Junhong Luo; Guobing Chen. "Insufficient epitope-specific T cell clones are responsible for impaired cellular immunity to inactivated SARS-CoV-2 vaccine in older adults." Nature aging (2023).

Epitope: ORF1a I2230T-2 2230-2238
Chemical type: Peptide from protein
Sequence: TIWFLLLSV
Position: 2230-2238
Source molecule (GenBank): QVE03131.1
Assay category: TCell
Result: Positive
Host:  18-85 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: ORF1a I2230T-2 2230-2238
Chemical type: Peptide from protein
Sequence: TIWFLLLSV
Position: 2230-2238
Source molecule (GenBank): QVE03131.1
Assay category: MhcBinding
Result: Positive

Epitope: ORF1a Ancestral 3673-3683
Chemical type: Peptide from protein
Sequence: SLSGFKLKDCV
Position: 3673-3683
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Host:  18-85 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: ORF1a Ancestral 3673-3683
Chemical type: Peptide from protein
Sequence: SLSGFKLKDCV
Position: 3673-3683
Source molecule (GenBank): P0DTD1.1
Assay category: MhcBinding
Result: Positive

Epitope: Spike ΔHV 69-70 62-70
Chemical type: Peptide from protein
Sequence: VTWFHAISG
Position: 62-70
Source molecule (GenBank): QSJ05725.1
Assay category: TCell
Result: Positive
Host:  18-85 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Spike ΔHV 69-70 62-70
Chemical type: Peptide from protein
Sequence: VTWFHAISG
Position: 62-70
Source molecule (GenBank): QSJ05725.1
Assay category: MhcBinding
Result: Positive

Epitope: Spike Ancestral 136-145
Chemical type: Peptide from protein
Sequence: CNDPFLGVYY
Position: 136-145
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Host:  18-85 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Spike Ancestral 136-145
Chemical type: Peptide from protein
Sequence: CNDPFLGVYY
Position: 136-145
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive

Epitope: ORF3a S253P 247-255
Chemical type: Peptide from protein
Sequence: HTIDGSPGV
Position: 248-256
Source molecule (GenBank): QXK95447.1
Assay category: TCell
Result: Negative
Host:  18-85 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: ORF3a S253P 247-255
Chemical type: Peptide from protein
Sequence: HTIDGSPGV
Position: 248-256
Source molecule (GenBank): QXK95447.1
Assay category: MhcBinding
Result: Positive

Epitope: S P26S 19-27
Chemical type: Peptide from protein
Sequence: TTRTQLPSA
Position: 19-27
Source molecule (GenBank): QRK43459.1
Assay category: TCell
Result: Negative
Host:  18-85 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: S P26S 19-27
Chemical type: Peptide from protein
Sequence: TTRTQLPSA
Position: 19-27
Source molecule (GenBank): QRK43459.1
Assay category: MhcBinding
Result: Positive

Epitope: N Ancestral 112-121
Chemical type: Peptide from protein
Sequence: YLGTGPEAGL
Position: 112-121
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Negative
Host:  18-85 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: N Ancestral 112-121
Chemical type: Peptide from protein
Sequence: YLGTGPEAGL
Position: 112-121
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Positive

Epitope: N A119S 112-121
Chemical type: Peptide from protein
Sequence: YLGTGPESGL
Position: 112-121
Source molecule (GenBank): QTQ43869.1
Assay category: TCell
Result: Negative
Host:  18-85 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: N A119S 112-121
Chemical type: Peptide from protein
Sequence: YLGTGPESGL
Position: 112-121
Source molecule (GenBank): QTQ43869.1
Assay category: MhcBinding
Result: Positive

Epitope: ORF1a Ancestral 265-274
Chemical type: Peptide from protein
Sequence: TFNGECPNFV
Position: 265-274
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Negative
Host:  18-85 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: ORF1a Ancestral 265-274
Chemical type: Peptide from protein
Sequence: TFNGECPNFV
Position: 265-274
Source molecule (GenBank): P0DTD1.1
Assay category: MhcBinding
Result: Positive

Epitope: ORF3a Ancestral 150-158
Chemical type: Peptide from protein
Sequence: HTNCYDYCI
Position: 150-158
Source molecule (GenBank): P0DTC3.1
Assay category: TCell
Result: Negative
Host:  18-85 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: ORF3a Ancestral 150-158
Chemical type: Peptide from protein
Sequence: HTNCYDYCI
Position: 150-158
Source molecule (GenBank): P0DTC3.1
Assay category: MhcBinding
Result: Positive

Epitope: N E367Q 361-369
Chemical type: Peptide from protein
Sequence: KTFPPTQPK
Position: 361-369
Source molecule (GenBank): QSL69376.1
Assay category: TCell
Result: Negative
Host:  18-85 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: N E367Q 361-369
Chemical type: Peptide from protein
Sequence: KTFPPTQPK
Position: 361-369
Source molecule (GenBank): QSL69376.1
Assay category: MhcBinding
Result: Positive

Epitope: ORF3a S26L 23-31
Chemical type: Peptide from protein
Sequence: ATPLDFVRA
Position: 23-31
Source molecule (GenBank): UHQ08572.1
Assay category: TCell
Result: Negative
Host:  18-85 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: ORF3a S26L 23-31
Chemical type: Peptide from protein
Sequence: ATPLDFVRA
Position: 23-31
Source molecule (GenBank): UHQ08572.1
Assay category: MhcBinding
Result: Positive

Epitope: S Ancestral 1069-1076
Chemical type: Peptide from protein
Sequence: PAQEKNFT
Position: 1069-1076
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Host:  18-85 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: S Ancestral 1069-1076
Chemical type: Peptide from protein
Sequence: PAQEKNFT
Position: 1069-1076
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive

Epitope: S T19R 19-27
Chemical type: Peptide from protein
Sequence: RTRTQLPPA
Position: 19-27
Source molecule (GenBank): UEO57867.1
Assay category: TCell
Result: Negative
Host:  18-85 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: S T19R 19-27
Chemical type: Peptide from protein
Sequence: RTRTQLPPA
Position: 19-27
Source molecule (GenBank): UEO57867.1
Assay category: MhcBinding
Result: Positive

Epitope: ORF1a Ancestral 2340-2349
Chemical type: Peptide from protein
Sequence: VLGLAAIMQL
Position: 2340-2349
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Host:  18-85 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: ORF1a Ancestral 2340-2349
Chemical type: Peptide from protein
Sequence: VLGLAAIMQL
Position: 2340-2349
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Host:  18-85 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: ORF1a Ancestral 2340-2349
Chemical type: Peptide from protein
Sequence: VLGLAAIMQL
Position: 2340-2349
Source molecule (GenBank): P0DTD1.1
Assay category: MhcBinding
Result: Positive

Epitope: ORF1a S2083I 2083-2090
Chemical type: Peptide from protein
Sequence: ILKITEEV
Position: 2083-2090
Source molecule (GenBank): UFL70378.1
Assay category: TCell
Result: Negative
Host:  18-85 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: ORF1a S2083I 2083-2090
Chemical type: Peptide from protein
Sequence: ILKITEEV
Position: 2083-2090
Source molecule (GenBank): UFL70378.1
Assay category: MhcBinding
Result: Positive

Epitope: ORF1a Δ2084 2076-2086
Chemical type: Peptide from protein
Sequence: ILKPANNSKI
Position: 2076-2085
Source molecule (GenBank): UFP26075.1
Assay category: TCell
Result: Negative
Host:  18-85 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: ORF1a Δ2084 2076-2086
Chemical type: Peptide from protein
Sequence: ILKPANNSKI
Position: 2076-2085
Source molecule (GenBank): UFP26075.1
Assay category: MhcBinding
Result: Positive

Epitope: ORF1a T3255I 3250-3261
Chemical type: Peptide from protein
Sequence: YQPPQISITSAV
Position: 3250-3261
Source molecule (GenBank): UDY65583.1
Assay category: TCell
Result: Negative
Host:  18-85 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: ORF1a T3255I 3250-3261
Chemical type: Peptide from protein
Sequence: YQPPQISITSAV
Position: 3250-3261
Source molecule (GenBank): UDY65583.1
Assay category: MhcBinding
Result: Positive

Epitope: ORF1b Ancestral 1561-1569
Chemical type: Peptide from protein
Sequence: GLCVDIPGI
Position: 5962-5970
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Negative
Host:  18-85 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: ORF1b Ancestral 1561-1569
Chemical type: Peptide from protein
Sequence: GLCVDIPGI
Position: 5962-5970
Source molecule (GenBank): P0DTD1.1
Assay category: MhcBinding
Result: Positive

Epitope: S Δ212 204-213
Chemical type: Peptide from protein
Sequence: YSKHTPINV
Position: 204-212
Source molecule (GenBank): QTM40532.1
Assay category: TCell
Result: Negative
Host:  18-85 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: S Δ212 204-213
Chemical type: Peptide from protein
Sequence: YSKHTPINV
Position: 204-212
Source molecule (GenBank): QTM40532.1
Assay category: MhcBinding
Result: Positive

Epitope: S G339D 332-341
Chemical type: Peptide from protein
Sequence: ITNLCPFDEV
Position: 332-341
Source molecule (GenBank): UYK54703.1
Assay category: TCell
Result: Negative
Host:  18-85 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: S G339D 332-341
Chemical type: Peptide from protein
Sequence: ITNLCPFDEV
Position: 332-341
Source molecule (GenBank): UYK54703.1
Assay category: MhcBinding
Result: Positive

Epitope: S Ancestral 761-770
Chemical type: Peptide from protein
Sequence: TQLNRALTGI
Position: 761-770
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Host:  18-85 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: S Ancestral 761-770
Chemical type: Peptide from protein
Sequence: TQLNRALTGI
Position: 761-770
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive

Epitope: S N969K 964-972
Chemical type: Peptide from protein
Sequence: KQLSSKFGA
Position: 964-972
Source molecule (GenBank): UYK54703.1
Assay category: TCell
Result: Negative
Host:  18-85 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: S N969K 964-972
Chemical type: Peptide from protein
Sequence: KQLSSKFGA
Position: 964-972
Source molecule (GenBank): UYK54703.1
Assay category: MhcBinding
Result: Positive

Epitope: M Ancestral 61-70
Chemical type: Peptide from protein
Sequence: TLACFVLAAV
Position: 61-70
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Negative
Host:  18-85 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: M Ancestral 61-70
Chemical type: Peptide from protein
Sequence: TLACFVLAAV
Position: 61-70
Source molecule (GenBank): P0DTC5.1
Assay category: MhcBinding
Result: Positive