Reference: Devi Gunasekera; Pooja Vir; Ahmad Faisal Karim; Margaret V Ragni; Kathleen P Pratt. "Hemophilia A subjects with an intron-22 gene inversion mutation show CD4<sup>+</sup> T-effector responses to multiple epitopes in FVIII." Frontiers in immunology (2023).

Epitope: FVIII F8 exon 22-23 junction region 2103-2122
Chemical type: Peptide from protein
Sequence: IMYSLDGKKWQTYRGNSTGT
Position: 2122-2141
Source molecule (GenBank): P00451.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: FVIII F8 exon 22-23 junction region 2103-2122
Chemical type: Peptide from protein
Sequence: IMYSLDGKKWQTYRGNSTGT
Position: 2122-2141
Source molecule (GenBank): P00451.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 61200.0

Epitope: FVIII F8 exon 22-23 junction region 2103-2122
Chemical type: Peptide from protein
Sequence: IMYSLDGKKWQTYRGNSTGT
Position: 2122-2141
Source molecule (GenBank): P00451.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 238070.0

Epitope: FVIII F8 exon 22-23 junction region 2103-2122
Chemical type: Peptide from protein
Sequence: IMYSLDGKKWQTYRGNSTGT
Position: 2122-2141
Source molecule (GenBank): P00451.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: FVIII F8 exon 22-23 junction region 2103-2122
Chemical type: Peptide from protein
Sequence: IMYSLDGKKWQTYRGNSTGT
Position: 2122-2141
Source molecule (GenBank): P00451.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: FVIII F8 exon 22-23 junction region 2103-2122
Chemical type: Peptide from protein
Sequence: IMYSLDGKKWQTYRGNSTGT
Position: 2122-2141
Source molecule (GenBank): P00451.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 57300.0

Epitope: FVIII F8 exon 22-23 junction region 2103-2122
Chemical type: Peptide from protein
Sequence: IMYSLDGKKWQTYRGNSTGT
Position: 2122-2141
Source molecule (GenBank): P00451.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: FVIII F8 exon 22-23 junction region 2103-2122
Chemical type: Peptide from protein
Sequence: IMYSLDGKKWQTYRGNSTGT
Position: 2122-2141
Source molecule (GenBank): P00451.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50580.0

Epitope: FVIII F8 exon 22-23 junction region 2103-2122
Chemical type: Peptide from protein
Sequence: IMYSLDGKKWQTYRGNSTGT
Position: 2122-2141
Source molecule (GenBank): P00451.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 50000.0

Epitope: FVIII F8 exon 22-23 junction region 2103-2122
Chemical type: Peptide from protein
Sequence: IMYSLDGKKWQTYRGNSTGT
Position: 2122-2141
Source molecule (GenBank): P00451.1
Assay category: MhcBinding
Result: Positive-Low
Quantitative measurement: 41950.0

Epitope: A2-10 FVIII 445-464
Chemical type: Peptide from protein
Sequence: ESGILGPLLYGEVGDTLLII
Position: 464-483
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host:  >18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: A2-12 FVIII 461-480
Chemical type: Peptide from protein
Sequence: LLIIFKNQASRPYNIYPHGI
Position: 480-499
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host:  >18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: A2-21 FVIII 529-548
Chemical type: Peptide from protein
Sequence: LTRYYSSFVNMERDLASGLI
Position: 548-567
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host:  >18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: A2-32 FVIII 613-632
Chemical type: Peptide from protein
Sequence: IMHSINGYVFDSLQLSVCLH
Position: 632-651
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host:  >18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: A2-33 FVIII 610-619
Chemical type: Peptide from protein
Sequence: ASNIMHSINGYVFDSLQLSV
Position: 629-648
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host:  >18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: A2-35 FVIII 629-648
Chemical type: Peptide from protein
Sequence: VCLHEVAYWYILSIGAQTDF
Position: 648-667
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host:  >18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: A2-38 FVIII 653-672
Chemical type: Peptide from protein
Sequence: QTDFLSVFFSGYTFKHKMVY
Position: 664-683
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host:  >18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: C2-13 FVIII 2265-2284
Chemical type: Peptide from protein
Sequence: SQDGHQWTLFFQNGKVKVFQ
Position: 2284-2303
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host:  >18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: C2-33 FVIII 2317-2331
Chemical type: Peptide from protein
Sequence: DPPLLTRYLRIHPQS
Position: 2317-2331
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Positive
Host:  >18 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: A2-63
Chemical type: Peptide from protein
Sequence: VFDSLQLSVCLHEVA
Position: 640-654
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host:  4-17 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: A2-64
Chemical type: Peptide from protein
Sequence: LQLSVCLHEVAYWYI
Position: 644-658
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host:  4-17 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: A2-72
Chemical type: Peptide from protein
Sequence: TFKHKMVYEDTLTLF
Position: 676-690
Source molecule (GenBank): P00451.1
Assay category: TCell
Result: Negative
Host:  4-17 years
Cell type: PBMC
Tissue: blood
Culture conditions: Short Term Restimulated