Reference: Carla Oseroff; Bjoern Peters; Valerie Pasquetto; Magdalini Moutaftsi; John Sidney; Vijay Panchanathan; David C Tscharke; Bernard Maillere; Howard Grey; Alessandro Sette. "Dissociation between epitope hierarchy and immunoprevalence in CD8 responses to vaccinia virus western reserve." Journal of immunology (Baltimore, Md. : 1950) (2008).

Epitope: NC peptide 1
Chemical type: Peptide from protein
Sequence: KYQQDRDTL
Position: 598-606
Source molecule (GenBank): AAO89427.1
Assay category: TCell
Result: Positive
Quantitative measurement: 365.0
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: NC peptide 1
Chemical type: Peptide from protein
Sequence: KYQQDRDTL
Position: 598-606
Source molecule (GenBank): AAO89427.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 53919.0

Epitope: NC peptide 1
Chemical type: Peptide from protein
Sequence: KYQQDRDTL
Position: 598-606
Source molecule (GenBank): AAO89427.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 6.4

Epitope: A12L (104–112)
Chemical type: Peptide from protein
Sequence: SYNAMDGQI
Position: 104-112
Source molecule (GenBank): Q80HV7.1
Assay category: TCell
Result: Positive
Quantitative measurement: 177.9
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A12L (104–112)
Chemical type: Peptide from protein
Sequence: SYNAMDGQI
Position: 104-112
Source molecule (GenBank): Q80HV7.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.1

Epitope: A12L (104–112)
Chemical type: Peptide from protein
Sequence: SYNAMDGQI
Position: 104-112
Source molecule (GenBank): Q80HV7.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 70000.0

Epitope: A24R (489–497)
Chemical type: Peptide from protein
Sequence: SVLSSITNI
Position: 489-497
Source molecule (GenBank): Q76ZP7.1
Assay category: TCell
Result: Positive
Quantitative measurement: 85.0
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A24R (489–497)
Chemical type: Peptide from protein
Sequence: SVLSSITNI
Position: 489-497
Source molecule (GenBank): Q76ZP7.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 76.0

Epitope: A24R (489–497)
Chemical type: Peptide from protein
Sequence: SVLSSITNI
Position: 489-497
Source molecule (GenBank): Q76ZP7.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 41894.0

Epitope: A33R (103–111)
Chemical type: Peptide from protein
Sequence: LYYQGSCYI
Position: 103-111
Source molecule (GenBank): P68617.1
Assay category: TCell
Result: Positive
Quantitative measurement: 295.0
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A33R (103–111)
Chemical type: Peptide from protein
Sequence: LYYQGSCYI
Position: 103-111
Source molecule (GenBank): P68617.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 2.8

Epitope: A33R (103–111)
Chemical type: Peptide from protein
Sequence: LYYQGSCYI
Position: 103-111
Source molecule (GenBank): P68617.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 70000.0

Epitope: H4L (698–706)
Chemical type: Peptide from protein
Sequence: KYYYSNSYL
Position: 698-706
Source molecule (GenBank): P68438.2
Assay category: TCell
Result: Positive
Quantitative measurement: 157.9
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: H4L (698–706)
Chemical type: Peptide from protein
Sequence: KYYYSNSYL
Position: 698-706
Source molecule (GenBank): P68438.2
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 70000.0

Epitope: H4L (698–706)
Chemical type: Peptide from protein
Sequence: KYYYSNSYL
Position: 698-706
Source molecule (GenBank): P68438.2
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 1.4

Epitope: AORF K (24–32)
Chemical type: Peptide from protein
Sequence: IPMFNKGHF
Position: 24-32
Source molecule (GenBank): P20520.1
Assay category: TCell
Result: Positive
Quantitative measurement: 85.0
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: AORF K (24–32)
Chemical type: Peptide from protein
Sequence: IPMFNKGHF
Position: 24-32
Source molecule (GenBank): P20520.1
Assay category: MhcBinding
Result: Positive-Intermediate
Quantitative measurement: 78.0

Epitope: AORF K (24–32)
Chemical type: Peptide from protein
Sequence: IPMFNKGHF
Position: 24-32
Source molecule (GenBank): P20520.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 45474.0

Epitope: AORF K (24–32)
Chemical type: Peptide from protein
Sequence: IPMFNKGHF
Position: 24-32
Source molecule (GenBank): P20520.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 70000.0

Epitope: A26L (185–193)
Chemical type: Peptide from protein
Sequence: SPMYLWFNV
Position: 185-193
Source molecule (GenBank): P24758.1
Assay category: TCell
Result: Positive
Quantitative measurement: 45.2
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: A26L (185–193)
Chemical type: Peptide from protein
Sequence: SPMYLWFNV
Position: 185-193
Source molecule (GenBank): P24758.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 11.0

Epitope: A26L (185–193)
Chemical type: Peptide from protein
Sequence: SPMYLWFNV
Position: 185-193
Source molecule (GenBank): P24758.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 17159.0

Epitope: A26L (185–193)
Chemical type: Peptide from protein
Sequence: SPMYLWFNV
Position: 185-193
Source molecule (GenBank): P24758.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 13672.0

Epitope: J4R (157–165)
Chemical type: Peptide from protein
Sequence: IPLKIVRFF
Position: 157-165
Source molecule (GenBank): P68609.1
Assay category: TCell
Result: Positive
Quantitative measurement: 92.5
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: J4R (157–165)
Chemical type: Peptide from protein
Sequence: IPLKIVRFF
Position: 157-165
Source molecule (GenBank): P68609.1
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 20.0

Epitope: J4R (157–165)
Chemical type: Peptide from protein
Sequence: IPLKIVRFF
Position: 157-165
Source molecule (GenBank): P68609.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 39317.0

Epitope: J4R (157–165)
Chemical type: Peptide from protein
Sequence: IPLKIVRFF
Position: 157-165
Source molecule (GenBank): P68609.1
Assay category: MhcBinding
Result: Negative
Quantitative measurement: 40825.0

Epitope: G9R (68–77)
Chemical type: Peptide from protein
Sequence: TGPGGLSALL
Position: 68-77
Source molecule (GenBank): P07611.3
Assay category: TCell
Result: Positive
Quantitative measurement: 37.3
Host:  6-12 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: G9R (68–77)
Chemical type: Peptide from protein
Sequence: TGPGGLSALL
Position: 68-77
Source molecule (GenBank): P07611.3
Assay category: MhcBinding
Result: Positive-High
Quantitative measurement: 8.6

Epitope: G9R (68–77)
Chemical type: Peptide from protein
Sequence: TGPGGLSALL
Position: 68-77
Source molecule (GenBank): P07611.3
Assay category: MhcBinding
Result: Negative