Reference: Rhea Y Busick; Carlos Aguilera; Anthony Quinn. "Dominant CTL-inducing epitopes on GAD65 are adjacent to or overlap with dominant Th-inducing epitopes." Clinical immunology (Orlando, Fla.) (2007).

Epitope: GAD-65 (13-23)
Chemical type: Peptide from protein
Sequence: SEDGSADPENP
Position: 13-23
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Negative
Host:  young
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GAD-65 (13-23)
Chemical type: Peptide from protein
Sequence: SEDGSADPENP
Position: 13-23
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD-65 (64-74)
Chemical type: Peptide from protein
Sequence: SRAATGKVACT
Position: 64-74
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Negative
Host:  young
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GAD-65 (64-74)
Chemical type: Peptide from protein
Sequence: SRAATGKVACT
Position: 64-74
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD-65 (112-122)
Chemical type: Peptide from protein
Sequence: QDVMNILLQYV
Position: 112-122
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Negative
Host:  young
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GAD-65 (112-122)
Chemical type: Peptide from protein
Sequence: QDVMNILLQYV
Position: 112-122
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Positive-Low

Epitope: GAD-65 (124-134)
Chemical type: Peptide from protein
Sequence: KSFDRSTKVID
Position: 124-134
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Negative
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GAD-65 (124-134)
Chemical type: Peptide from protein
Sequence: KSFDRSTKVID
Position: 124-134
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Positive
Host:  young
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GAD-65 (124-134)
Chemical type: Peptide from protein
Sequence: KSFDRSTKVID
Position: 124-134
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD-65 (133-143)
Chemical type: Peptide from protein
Sequence: IDFHYPNELLQ
Position: 133-143
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Negative
Host:  young
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GAD-65 (133-143)
Chemical type: Peptide from protein
Sequence: IDFHYPNELLQ
Position: 133-143
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD-65 (148-158)
Chemical type: Peptide from protein
Sequence: ELADQPQNLEE
Position: 148-158
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Negative
Host:  young
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GAD-65 (148-158)
Chemical type: Peptide from protein
Sequence: ELADQPQNLEE
Position: 148-158
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD-65 (157-167)
Chemical type: Peptide from protein
Sequence: EEILTHCQTTL
Position: 157-167
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Negative
Host:  young
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GAD-65 (157-167)
Chemical type: Peptide from protein
Sequence: EEILTHCQTTL
Position: 157-167
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD-65 (274-284)
Chemical type: Peptide from protein
Sequence: IAFTSEHSHFS
Position: 274-284
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Negative
Host:  young
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GAD-65 (274-284)
Chemical type: Peptide from protein
Sequence: IAFTSEHSHFS
Position: 274-284
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD-65 (283-293)
Chemical type: Peptide from protein
Sequence: FSLKKGAAALG
Position: 283-293
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Negative
Host:  young
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GAD-65 (283-293)
Chemical type: Peptide from protein
Sequence: FSLKKGAAALG
Position: 283-293
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD-65 (289-299)
Chemical type: Peptide from protein
Sequence: AAALGIGTDSV
Position: 289-299
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Negative
Host:  young
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GAD-65 (289-299)
Chemical type: Peptide from protein
Sequence: AAALGIGTDSV
Position: 289-299
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD-65 (331-341)
Chemical type: Peptide from protein
Sequence: LVSATAGTTVY
Position: 331-341
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Negative
Host:  young
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GAD-65 (331-341)
Chemical type: Peptide from protein
Sequence: LVSATAGTTVY
Position: 331-341
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD-65 (337-347)
Chemical type: Peptide from protein
Sequence: GTTVYGAFDPL
Position: 337-347
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Negative
Host:  young
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GAD-65 (337-347)
Chemical type: Peptide from protein
Sequence: GTTVYGAFDPL
Position: 337-347
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD-65 (340-350)
Chemical type: Peptide from protein
Sequence: VYGAFDPLLAV
Position: 340-350
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Negative
Host:  young
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GAD-65 (340-350)
Chemical type: Peptide from protein
Sequence: VYGAFDPLLAV
Position: 340-350
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD-65 (358-368)
Chemical type: Peptide from protein
Sequence: KIWMHVDAAWG
Position: 358-368
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Negative
Host:  young
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GAD-65 (358-368)
Chemical type: Peptide from protein
Sequence: KIWMHVDAAWG
Position: 358-368
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD-65 (421-431)
Chemical type: Peptide from protein
Sequence: MHASYLFQQDK
Position: 421-431
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Negative
Host:  young
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GAD-65 (421-431)
Chemical type: Peptide from protein
Sequence: MHASYLFQQDK
Position: 421-431
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD-65 (439-449)
Chemical type: Peptide from protein
Sequence: TGDKALQCGRH
Position: 439-449
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Negative
Host:  young
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GAD-65 (439-449)
Chemical type: Peptide from protein
Sequence: TGDKALQCGRH
Position: 439-449
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD-65 (475-485)
Chemical type: Peptide from protein
Sequence: LELAEYLYTII
Position: 475-485
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Negative
Host:  young
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GAD-65 (475-485)
Chemical type: Peptide from protein
Sequence: LELAEYLYTII
Position: 475-485
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD-65 (493-503)
Chemical type: Peptide from protein
Sequence: MVFDGKPQHTN
Position: 493-503
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Negative
Host:  young
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GAD-65 (493-503)
Chemical type: Peptide from protein
Sequence: MVFDGKPQHTN
Position: 493-503
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD-65 (508-518)
Chemical type: Peptide from protein
Sequence: FVPPSLRTLED
Position: 508-518
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Negative
Host:  young
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GAD-65 (508-518)
Chemical type: Peptide from protein
Sequence: FVPPSLRTLED
Position: 508-518
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD-65 (532-542)
Chemical type: Peptide from protein
Sequence: VIKARMMEYGT
Position: 532-542
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Negative
Host:  young
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GAD-65 (532-542)
Chemical type: Peptide from protein
Sequence: VIKARMMEYGT
Position: 532-542
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Negative

Epitope: GAD-65 (546-554)
Chemical type: Peptide from protein
Sequence: SYQPLGDKV
Position: 546-554
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Positive
Host:  young
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Cell Line / Clone

Epitope: GAD-65 (546-554)
Chemical type: Peptide from protein
Sequence: SYQPLGDKV
Position: 546-554
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Positive-Low
Host:  young
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GAD-65 (546-554)
Chemical type: Peptide from protein
Sequence: SYQPLGDKV
Position: 546-554
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Positive
Host: F 6 weeks
Cell type: splenocyte
Tissue: spleen
Culture conditions: Short Term Restimulated

Epitope: GAD-65 (546-554)
Chemical type: Peptide from protein
Sequence: SYQPLGDKV
Position: 546-554
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Positive
Host:  young
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Cell Line / Clone

Epitope: GAD-65 (546-554)
Chemical type: Peptide from protein
Sequence: SYQPLGDKV
Position: 546-554
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Positive
Host:  young
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Cell Line / Clone

Epitope: GAD-65 (546-554)
Chemical type: Peptide from protein
Sequence: SYQPLGDKV
Position: 546-554
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Positive
Host:  young

Epitope: GAD-65 (546-554)
Chemical type: Peptide from protein
Sequence: SYQPLGDKV
Position: 546-554
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Positive
Host:  young
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Cell Line / Clone

Epitope: GAD-65 (546-554)
Chemical type: Peptide from protein
Sequence: SYQPLGDKV
Position: 546-554
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Positive
Host:  young
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Cell Line / Clone

Epitope: GAD-65 (546-554)
Chemical type: Peptide from protein
Sequence: SYQPLGDKV
Position: 546-554
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Positive-Low
Host:  young
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Cell Line / Clone

Epitope: GAD-65 (546-554)
Chemical type: Peptide from protein
Sequence: SYQPLGDKV
Position: 546-554
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Positive
Host:  young
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Cell Line / Clone

Epitope: GAD-65 (546-554)
Chemical type: Peptide from protein
Sequence: SYQPLGDKV
Position: 546-554
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Positive
Host:  young
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Cell Line / Clone

Epitope: GAD-65 (546-554)
Chemical type: Peptide from protein
Sequence: SYQPLGDKV
Position: 546-554
Source molecule (GenBank): P48320.1
Assay category: TCell
Result: Negative
Host:  young
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Cell Line / Clone

Epitope: GAD-65 (546-554)
Chemical type: Peptide from protein
Sequence: SYQPLGDKV
Position: 546-554
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Positive

Epitope: GAD-65 (530-554)
Chemical type: Peptide from protein
Sequence: APVIKARMMEYGTTMVSYQPLGDKV
Position: 530-554
Source molecule (GenBank): P48320.1
Assay category: MhcBinding
Result: Negative