Reference: Michal Bassani-Sternberg; Eva Bräunlein; Richard Klar; Thomas Engleitner; Pavel Sinitcyn; Stefan Audehm; Melanie Straub; Julia Weber; Julia Slotta-Huspenina; Katja Specht; Marc E Martignoni; Angelika Werner; Rüdiger Hein; Dirk H Busch; Christian Peschel; Roland Rad; Jürgen Cox; Matthias Mann; Angela M Krackhardt. "Direct identification of clinically relevant neoepitopes presented on native human melanoma tissue by mass spectrometry." Nature communications (2016).

Epitope: TYR
Chemical type: Peptide from protein
Sequence: AKHTISSDYVIPIGT
Position: 141-155
Source molecule (GenBank): AAK00805.1
Assay category: MhcLigandElution
Result: Positive

Epitope: TYR
Chemical type: Peptide from protein
Sequence: AKHTISSDYVIPIGTYG
Position: 141-157
Source molecule (GenBank): AAK00805.1
Assay category: MhcLigandElution
Result: Positive

Epitope: MLANA
Chemical type: Peptide from protein
Sequence: APPAYEKLSAEQSPPPY
Position: 100-116
Source molecule (GenBank): NP_005502.1
Assay category: MhcLigandElution
Result: Positive

Epitope: FLNA
Chemical type: Peptide from protein
Sequence: ATPTSPIRVK
Position: 829-838
Source molecule (GenBank): BAD52436.1
Assay category: MhcLigandElution
Result: Positive

Epitope: TYRP1
Chemical type: Peptide from protein
Sequence: AVTADSRPHSPQYPH
Position: 67-81
Source molecule (GenBank): AAC41924.1
Assay category: MhcLigandElution
Result: Positive

Epitope: TYRP1
Chemical type: Peptide from protein
Sequence: DQRVLIVRRNLLDLSKE
Position: 123-139
Source molecule (GenBank): CAG28611.1
Assay category: MhcLigandElution
Result: Positive

Epitope: TYRP1
Chemical type: Peptide from protein
Sequence: DQRVLIVRRNLLDLSKEEKN
Position: 123-142
Source molecule (GenBank): CAG28611.1
Assay category: MhcLigandElution
Result: Positive

Epitope: SRRM1
Chemical type: Peptide from protein
Sequence: EARSPQPNKR
Position: 670-679
Source molecule (GenBank): AAP97290.1
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 211 230 190 197 306 629

Epitope: TYRP1
Chemical type: Peptide from protein
Sequence: EAVTADSRPHSPQYPH
Position: 66-81
Source molecule (GenBank): AAC41924.1
Assay category: MhcLigandElution
Result: Positive

Epitope: MLANA
Chemical type: Peptide from protein
Sequence: EPVVPNAPP
Position: 94-102
Source molecule (GenBank): NP_005502.1
Assay category: MhcLigandElution
Result: Positive

Epitope: MAGEA1
Chemical type: Peptide from protein
Sequence: EVYDGREHSAY
Position: 222-232
Source molecule (GenBank): NP_004979.3
Assay category: MhcLigandElution
Result: Positive

Epitope: PMEL
Chemical type: Peptide from protein
Sequence: FLRNQPLTF
Position: 239-247
Source molecule (GenBank): AAA35930.1
Assay category: MhcLigandElution
Result: Positive

Epitope: PMEL
Chemical type: Peptide from protein
Sequence: FLRNQPLTF
Position: 239-247
Source molecule (GenBank): AAA35930.1
Assay category: MhcLigandElution
Result: Positive

Epitope: RBM14
Chemical type: Peptide from protein
Sequence: FRRSPTKSS
Position: 624-632
Source molecule (GenBank): AAK77961.1
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 142 215 251 252 1 3

Epitope: RBM14
Chemical type: Peptide from protein
Sequence: FRRSPTKSSL
Position: 624-633
Source molecule (GenBank): AAK77961.1
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 142 215 251 252 1 3

Epitope: ARID5B
Chemical type: Peptide from protein
Sequence: GIMSPLAKK
Position: 307-315
Source molecule (GenBank): NP_001306014.1
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 211 230 190 197 306 629

Epitope: ATP2A1
Chemical type: Peptide from protein
Sequence: GIVATTGVGTE
Position: 221-231
Source molecule (GenBank): NP_004311.1
Assay category: MhcLigandElution
Result: Positive

Epitope: PMEL
Chemical type: Peptide from protein
Sequence: GQVSLKVSNDGPTLIG
Position: 71-86
Source molecule (GenBank): AAA35930.1
Assay category: MhcLigandElution
Result: Positive

Epitope: CBX4
Chemical type: Peptide from protein
Sequence: GSRKLSPTKEAF
Position: 344-355
Source molecule (GenBank): NP_003646.2
Assay category: MhcLigandElution
Result: Positive

Epitope: TYRP1
Chemical type: Peptide from protein
Sequence: GTASYLIRAR
Position: 495-504
Source molecule (GenBank): CAG28611.1
Assay category: MhcLigandElution
Result: Positive

Epitope: PMEL
Chemical type: Peptide from protein
Sequence: GTATLRLVK
Position: 467-475
Source molecule (GenBank): AAA35930.1
Assay category: MhcLigandElution
Result: Positive

Epitope: MAP3K11
Chemical type: Peptide from protein
Sequence: GTVSPPPGTSR
Position: 737-747
Source molecule (GenBank): AAH64543.1
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 211 230 190 197 306 629

Epitope: PIM1
Chemical type: Peptide from protein
Sequence: HSLSPGPSK
Position: 396-404
Source molecule (GenBank): NP_001230115.1
Assay category: MhcLigandElution
Result: Positive

Epitope: SEC24D
Chemical type: Peptide from protein
Sequence: HTASPTGMMK
Position: 34-43
Source molecule (GenBank): AAD28756.2
Assay category: MhcLigandElution
Result: Positive

Epitope: GAS7
Chemical type: Peptide from protein
Sequence: IPASPGSHR
Position: 126-134
Source molecule (GenBank): AAG26329.1
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 211 230 190 197 306 629

Epitope: TYR
Chemical type: Peptide from protein
Sequence: ISSKDLGYDYSY
Position: 440-451
Source molecule (GenBank): AAK00805.1
Assay category: MhcLigandElution
Result: Positive

Epitope: TYR
Chemical type: Peptide from protein
Sequence: ISSKDLGYDYSY
Position: 440-451
Source molecule (GenBank): AAK00805.1
Assay category: MhcLigandElution
Result: Positive

Epitope: TYR
Chemical type: Peptide from protein
Sequence: ISSKDLGYDYSY
Position: 440-451
Source molecule (GenBank): AAK00805.1
Assay category: MhcLigandElution
Result: Positive

Epitope: PMEL
Chemical type: Peptide from protein
Sequence: ITDQVPFSV
Position: 216-224
Source molecule (GenBank): AAA35930.1
Assay category: MhcLigandElution
Result: Positive

Epitope: PMEL
Chemical type: Peptide from protein
Sequence: ITDQVPFSV
Position: 216-224
Source molecule (GenBank): AAA35930.1
Assay category: MhcLigandElution
Result: Positive

Epitope: PKN1
Chemical type: Peptide from protein
Sequence: KIFSKQQGKAF
Position: 498-508
Source molecule (GenBank): NP_998725.1
Assay category: MhcLigandElution
Result: Positive

Epitope: BIRC5
Chemical type: Peptide from protein
Sequence: KLDRERAKNK
Position: 126-135
Source molecule (GenBank): NP_001012271.1
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 211 230 190 197 306 629

Epitope: PPFIBP1
Chemical type: Peptide from protein
Sequence: KSSSLGNLK
Position: 446-454
Source molecule (GenBank): AAC26103.1
Assay category: MhcLigandElution
Result: Positive

Epitope: PPFIBP1
Chemical type: Peptide from protein
Sequence: KSSSLGNLKK
Position: 446-455
Source molecule (GenBank): AAC26103.1
Assay category: MhcLigandElution
Result: Positive

Epitope: MAGEA1
Chemical type: Peptide from protein
Sequence: KVLEYVIKV
Position: 278-286
Source molecule (GenBank): NP_004979.3
Assay category: MhcLigandElution
Result: Positive

Epitope: TYR
Chemical type: Peptide from protein
Sequence: LMEKEDYHSLY
Position: 515-525
Source molecule (GenBank): AAK00805.1
Assay category: MhcLigandElution
Result: Positive

Epitope: TYR
Chemical type: Peptide from protein
Sequence: LMEKEDYHSLY
Position: 515-525
Source molecule (GenBank): AAK00805.1
Assay category: MhcLigandElution
Result: Positive

Epitope: CEP63
Chemical type: Peptide from protein
Sequence: LPVSPLGSIATR
Position: 654-665
Source molecule (GenBank): NP_079456.2
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 211 230 190 197 306 629

Epitope: TYR
Chemical type: Peptide from protein
Sequence: LRWEQEIQKL
Position: 216-225
Source molecule (GenBank): AAK00805.1
Assay category: MhcLigandElution
Result: Positive

Epitope: PRAME
Chemical type: Peptide from protein
Sequence: LSHIHASSY
Position: 267-275
Source molecule (GenBank): AAH39731.1
Assay category: MhcLigandElution
Result: Positive

Epitope: PRAME
Chemical type: Peptide from protein
Sequence: LSHIHASSY
Position: 267-275
Source molecule (GenBank): AAH39731.1
Assay category: MhcLigandElution
Result: Positive

Epitope: MAGEC1
Chemical type: Peptide from protein
Sequence: NSSPPRYEF
Position: 1061-1069
Source molecule (GenBank): AAC24227.1
Assay category: MhcLigandElution
Result: Positive

Epitope: STPG2
Chemical type: Peptide from protein
Sequence: NTEESPRGLRTY
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 142 215 251 252 1 3

Epitope: ASPM
Chemical type: Peptide from protein
Sequence: NVTTPISKR
Position: 508-516
Source molecule (GenBank): AAM48745.1
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 211 230 190 197 306 629

Epitope: MLANA
Chemical type: Peptide from protein
Sequence: PVVPNAPPA
Position: 95-103
Source molecule (GenBank): NP_005502.1
Assay category: MhcLigandElution
Result: Positive

Epitope: DCT
Chemical type: Peptide from protein
Sequence: QRLIGNESF
Position: 232-240
Source molecule (GenBank): NP_001913.2
Assay category: MhcLigandElution
Result: Positive

Epitope: CCDC110
Chemical type: Peptide from protein
Sequence: QTDPDVHRNGKY
Position: 270-281
Source molecule (GenBank): NP_689988.1
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 142 215 251 252 1 3

Epitope: CARD11
Chemical type: Peptide from protein
Sequence: RAKSPISLK
Position: 502-510
Source molecule (GenBank): AAL34460.1
Assay category: MhcLigandElution
Result: Positive

Epitope: PMEL
Chemical type: Peptide from protein
Sequence: RGSRSYVPLAHSSS
Position: 199-212
Source molecule (GenBank): AAA35930.1
Assay category: MhcLigandElution
Result: Positive

Epitope: DYRK1A
Chemical type: Peptide from protein
Sequence: RIYQYIQSRF
Position: 317-326
Source molecule (GenBank): AAB18639.1
Assay category: MhcLigandElution
Result: Positive

Epitope: ATAD2
Chemical type: Peptide from protein
Sequence: RLRSAGAAQKK
Position: 38-48
Source molecule (GenBank): EAW92033.1
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 211 230 190 197 306 629

Epitope: PRAME
Chemical type: Peptide from protein
Sequence: RLVELAGQSL
Position: 25-34
Source molecule (GenBank): AAH39731.1
Assay category: MhcLigandElution
Result: Positive

Epitope: PRAME
Chemical type: Peptide from protein
Sequence: RLVELAGQSL
Position: 25-34
Source molecule (GenBank): AAH39731.1
Assay category: MhcLigandElution
Result: Positive

Epitope: TYRP1
Chemical type: Peptide from protein
Sequence: RPHSPQYPH
Position: 73-81
Source molecule (GenBank): AAC41924.1
Assay category: MhcLigandElution
Result: Positive

Epitope: MIIP
Chemical type: Peptide from protein
Sequence: RPRPHSAPSL
Position: 108-117
Source molecule (GenBank): EAW71729.1
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 142 215 251 252 1 3

Epitope: STRADA
Chemical type: Peptide from protein
Sequence: RPVTPITNF
Position: 358-366
Source molecule (GenBank): NP_001003786.1
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 142 211 251 252 1 3

Epitope: KLF10
Chemical type: Peptide from protein
Sequence: RPVTPVSDL
Position: 52-60
Source molecule (GenBank): NP_001027453.1
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 142 211 251 252 1 3

Epitope: MKL1
Chemical type: Peptide from protein
Sequence: RQNSTSLTGK
Position: 330-339
Source molecule (GenBank): AAI15040.1
Assay category: MhcLigandElution
Result: Positive

Epitope: FILIP1L
Chemical type: Peptide from protein
Sequence: RRISDPQVF
Position: 788-796
Source molecule (GenBank): AAV34207.1
Assay category: MhcLigandElution
Result: Positive

Epitope: HAT1
Chemical type: Peptide from protein
Sequence: RRLISPYKKK
Position: 357-366
Source molecule (GenBank): NP_003633.1
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 211 230 190 197 306 629

Epitope: UBD
Chemical type: Peptide from protein
Sequence: RRSSSVAQVKA
Position: 107-117
Source molecule (GenBank): AAD52982.1
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 211 230 190 197 306 629

Epitope: SH3PXD2A
Chemical type: Peptide from protein
Sequence: RRWTLGGMVNR
Position: 253-263
Source molecule (GenBank): Q5TCZ1.1
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 211 230 190 197 306 629

Epitope: TCEB3
Chemical type: Peptide from protein
Sequence: RSYSPDHRQK
Position: 15-24
Source molecule (GenBank): AAO15305.1
Assay category: MhcLigandElution
Result: Positive

Epitope: HIF1A
Chemical type: Peptide from protein
Sequence: RTASPNRAGK
Position: 665-674
Source molecule (GenBank): AAC68568.1
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 211 230 190 197 306 629

Epitope: TRIM2
Chemical type: Peptide from protein
Sequence: RVKSPGSGHVK
Position: 437-447
Source molecule (GenBank): NP_001123539.1
Assay category: MhcLigandElution
Result: Positive

Epitope: MAGEA6
Chemical type: Peptide from protein
Sequence: SIFGDPKKLLTQY
Position: 237-249
Source molecule (GenBank): CAG46567.1
Assay category: MhcLigandElution
Result: Positive

Epitope: PMEL
Chemical type: Peptide from protein
Sequence: SSSAFTITDQVPFSVSVSQLR
Position: 210-230
Source molecule (GenBank): AAA35930.1
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 142 215 251 252 1 3

Epitope: MAGEA12
Chemical type: Peptide from protein
Sequence: SVFAHPRKL
Position: 237-245
Source molecule (GenBank): AAH63681.1
Assay category: MhcLigandElution
Result: Positive

Epitope: APOOL
Chemical type: Peptide from protein
Sequence: SVPLPTELS
Position: 220-228
Source molecule (GenBank): NP_940852.3
Assay category: MhcLigandElution
Result: Positive

Epitope: SRSF5
Chemical type: Peptide from protein
Sequence: SVSRSPVPEK
Position: 228-237
Source molecule (GenBank): EAW81003.1
Assay category: MhcLigandElution
Result: Positive

Epitope: BSN
Chemical type: Peptide from protein
Sequence: TGPTTVPATK
Position: 2928-2937
Source molecule (GenBank): AAC83555.1
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 211 230 190 197 306 629

Epitope: WDR89
Chemical type: Peptide from protein
Sequence: TRLNIQDVREVVNMKE
Position: 256-271
Source molecule (GenBank): NP_542397.1
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 211 230 190 197 306 629

Epitope: TYRP1
Chemical type: Peptide from protein
Sequence: VLIVRRNLLDLSKEE
Position: 126-140
Source molecule (GenBank): CAG28611.1
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 142 215 251 252 1 3

Epitope: TYRP1
Chemical type: Peptide from protein
Sequence: VLIVRRNLLDLSKEE
Position: 126-140
Source molecule (GenBank): CAG28611.1
Assay category: MhcLigandElution
Result: Positive

Epitope: TXNDC11
Chemical type: Peptide from protein
Sequence: VSLVHSGSVY
Position: 311-320
Source molecule (GenBank): NP_001290376.1
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 142 252 1

Epitope: TYRP1
Chemical type: Peptide from protein
Sequence: VTADSRPHSPQYPH
Position: 68-81
Source molecule (GenBank): AAC41924.1
Assay category: MhcLigandElution
Result: Positive

Epitope: NIPSNAP3A
Chemical type: Peptide from protein
Sequence: VVAAVRESVN
Position: 169-178
Source molecule (GenBank): NP_001316499.1
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 211 230 190 197 306 629

Epitope: LSR
Chemical type: Peptide from protein
Sequence: VVATKQGNAVT
Position: 138-148
Source molecule (GenBank): AAB58317.1
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 211 230 190 197 306 629

Epitope: DYRK2
Chemical type: Peptide from protein
Sequence: VYTYIQSRF
Position: 283-291
Source molecule (GenBank): AAF91393.1
Assay category: MhcLigandElution
Result: Positive

Epitope: VIM
Chemical type: Peptide from protein
Sequence: YASSPGGVYATR
Position: 53-64
Source molecule (GenBank): ACA06103.1
Assay category: MhcLigandElution
Result: Positive
MHC allele types: 211 230 190 197 306 629

Epitope: RBPMS (P46L)
Chemical type: Peptide, no natural source
Sequence: RLFKGYEGSLIK
Assay category: TCell
Result: Negative
Host: M 63 years
Cell type: T cell
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: RBPMS (P46L)
Chemical type: Peptide, no natural source
Sequence: RLFKGYEGSLIK
Assay category: MhcLigandElution
Result: Positive

Epitope: SYTL4 (wlid type)
Chemical type: Peptide from protein
Sequence: GRIAFSLKY
Position: 358-366
Source molecule (GenBank): Q96C24.2
Assay category: TCell
Result: Negative
Host: M 63 years
Cell type: T cell
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: NCAPG2 (wlid type)
Chemical type: Peptide from protein
Sequence: KPILWRGLK
Position: 124-132
Source molecule (GenBank): AAH20560.1
Assay category: TCell
Result: Negative
Host: M 63 years
Cell type: T cell
Tissue: blood
Culture conditions: Direct Ex Vivo