Reference: Friederike Ebner; Eliot Morrison; Miriam Bertazzon; Ankur Midha; Susanne Hartmann; Christian Freund; Miguel Álvaro-Benito. "CD4<sup>+</sup> T<sub>h</sub> immunogenicity of the . secreted products." NPJ vaccines (2020).

Epitope: FKGPNGYISPWHDIPLFADEAKK
Chemical type: Peptide from protein
Sequence: FKGPNGYISPWHDIPLFADEAKK
Position: 119-141
Source molecule (GenBank): F1L719
Assay category: MhcLigandElution
Result: Positive

Epitope: FKGPNGYISPWHDIPLFADEAKK
Chemical type: Peptide from protein
Sequence: FKGPNGYISPWHDIPLFADEAKK
Position: 119-141
Source molecule (GenBank): F1L719
Assay category: MhcLigandElution
Result: Positive

Epitope: ATIEKVRALGANIGM
Chemical type: Peptide from protein
Sequence: ATIEKVRALGANIGM
Position: 95-109
Source molecule (GenBank): F1LE63.1
Assay category: MhcLigandElution
Result: Positive

Epitope: ATIEKVRALGANIGM
Chemical type: Peptide from protein
Sequence: ATIEKVRALGANIGM
Position: 95-109
Source molecule (GenBank): F1LE63.1
Assay category: MhcLigandElution
Result: Positive

Epitope: GDVLQYKDSDFEDSIKG
Chemical type: Peptide from protein
Sequence: GDVLQYKDSDFEDSIKG
Position: 17-33
Source molecule (GenBank): F1KXK9.1
Assay category: MhcLigandElution
Result: Positive

Epitope: LRQQAVAIKGKLLCGTAPA
Chemical type: Peptide from protein
Sequence: LRQQAVAIKGKLLCGTAPA
Position: 26-44
Source molecule (GenBank): F1LEX9
Assay category: MhcLigandElution
Result: Positive

Epitope: LKQIATAILASGKGILAADESNPS
Chemical type: Peptide from protein
Sequence: LKQIATAILASGKGILAADESNPS
Position: 17-40
Source molecule (GenBank): F1KQJ9
Assay category: MhcLigandElution
Result: Positive

Epitope: LKQIATAILASGKGILAADESNPS
Chemical type: Peptide from protein
Sequence: LKQIATAILASGKGILAADESNPS
Position: 17-40
Source molecule (GenBank): F1KQJ9
Assay category: MhcLigandElution
Result: Positive

Epitope: QQALEAAKSAAVGSVPQ
Chemical type: Peptide from protein
Sequence: QQALEAAKSAAVGSVPQ
Position: 76-92
Source molecule (GenBank): Q93122
Assay category: MhcLigandElution
Result: Positive

Epitope: QQALEAAKSAAVGSVPQ
Chemical type: Peptide from protein
Sequence: QQALEAAKSAAVGSVPQ
Position: 76-92
Source molecule (GenBank): Q93122
Assay category: MhcLigandElution
Result: Positive

Epitope: PNAIANQIKE
Chemical type: Peptide from protein
Sequence: PNAIANQIKE
Position: 131-140
Source molecule (GenBank): F1KU32.1
Assay category: MhcLigandElution
Result: Positive

Epitope: DAALDALKEKSEKLHAK
Chemical type: Peptide from protein
Sequence: DAALDALKEKSEKLHAK
Position: 70-86
Source molecule (GenBank): F1LEI7.1
Assay category: MhcLigandElution
Result: Positive

Epitope: VEEKVEQQEQTE
Chemical type: Peptide from protein
Sequence: VEEKVEQQEQTE
Assay category: MhcLigandElution
Result: Positive

Epitope: SSLLQPNDVCSVVAQESHGTVF
Chemical type: Peptide from protein
Sequence: SSLLQPNDVCSVVAQESHGTVF
Position: 3048-3069
Source molecule (GenBank): F1KPM2.1
Assay category: MhcLigandElution
Result: Positive

Epitope: YGEENEVGNIEPYLL
Chemical type: Peptide from protein
Sequence: YGEENEVGNIEPYLL
Position: 54-68
Source molecule (GenBank): F1LHW8.1
Assay category: MhcLigandElution
Result: Positive

Epitope: YVALWYKHGQPVFGRAYPD
Chemical type: Peptide from protein
Sequence: YVALWYKHGQPVFGRAYPD
Position: 217-235
Source molecule (GenBank): Q7YXK2.1
Assay category: MhcLigandElution
Result: Positive

Epitope: NGSNENEATYADMFYEGIRDLHM
Chemical type: Peptide from protein
Sequence: NGSNENEATYADMFYEGIRDLHM
Position: 37-59
Source molecule (GenBank): F1L8V4.1
Assay category: MhcLigandElution
Result: Positive

Epitope: NISNSIPVIRG
Chemical type: Peptide from protein
Sequence: NISNSIPVIRG
Position: 24-34
Source molecule (GenBank): F1KSI5.1
Assay category: MhcLigandElution
Result: Positive

Epitope: LAEFNDAFSKLISESSLSDEAKL
Chemical type: Peptide from protein
Sequence: LAEFNDAFSKLISESSLSDEAKL
Assay category: MhcLigandElution
Result: Positive

Epitope: ATQLNEYKIVASYEVR
Chemical type: Peptide from protein
Sequence: ATQLNEYKIVASYEVR
Position: 1316-1331
Source molecule (GenBank): F1KQS3.1
Assay category: MhcLigandElution
Result: Positive

Epitope: YVYDVTSTASLQLMQGK
Chemical type: Peptide from protein
Sequence: YVYDVTSTASLQLMQGK
Position: 57-73
Source molecule (GenBank): F1KPM2.1
Assay category: MhcLigandElution
Result: Positive

Epitope: FIPFGSNEIQE
Chemical type: Peptide from protein
Sequence: FIPFGSNEIQE
Position: 87-97
Source molecule (GenBank): F1LF53.1
Assay category: MhcLigandElution
Result: Positive

Epitope: NEWRGSDSETIGAIADE
Chemical type: Peptide from protein
Sequence: NEWRGSDSETIGAIADE
Position: 3582-3598
Source molecule (GenBank): F1KPH3
Assay category: MhcLigandElution
Result: Positive

Epitope: YSGLNKHVPAVYIENDE
Chemical type: Peptide from protein
Sequence: YSGLNKHVPAVYIENDE
Assay category: MhcLigandElution
Result: Positive

Epitope: SNLTENIQKIQEDIDE
Chemical type: Peptide from protein
Sequence: SNLTENIQKIQEDIDE
Assay category: MhcLigandElution
Result: Positive

Epitope: ISGIQDFSMFGIPYVG
Chemical type: Peptide from protein
Sequence: ISGIQDFSMFGIPYVG
Position: 42-57
Source molecule (GenBank): F1KX94.1
Assay category: MhcLigandElution
Result: Positive

Epitope: LSNDQGTYLDFASLRDL
Chemical type: Peptide from protein
Sequence: LSNDQGTYLDFASLRDL
Position: 2766-2782
Source molecule (GenBank): F1KPP1
Assay category: MhcLigandElution
Result: Positive

Epitope: IDSSLSMTKEGLIQ
Chemical type: Peptide from protein
Sequence: IDSSLSMTKEGLIQ
Position: 53-66
Source molecule (GenBank): F1L8I9.1
Assay category: MhcLigandElution
Result: Positive

Epitope: LVHPYYSISEQINPGAHFDI
Chemical type: Peptide from protein
Sequence: LVHPYYSISEQINPGAHFDI
Position: 59-78
Source molecule (GenBank): F1L7U1
Assay category: MhcLigandElution
Result: Positive

Epitope: LVHPYYSISEQINPGAHFDI
Chemical type: Peptide from protein
Sequence: LVHPYYSISEQINPGAHFDI
Position: 59-78
Source molecule (GenBank): F1L7U1
Assay category: MhcLigandElution
Result: Positive

Epitope: GAFYTFSRNHNDIGQIRQD
Chemical type: Peptide from protein
Sequence: GAFYTFSRNHNDIGQIRQD
Position: 2068-2086
Source molecule (GenBank): F1KPW3.1
Assay category: MhcLigandElution
Result: Positive

Epitope: VKSSNNSNYTVTPNMGIVE
Chemical type: Peptide from protein
Sequence: VKSSNNSNYTVTPNMGIVE
Position: 38-56
Source molecule (GenBank): F1LGP8
Assay category: MhcLigandElution
Result: Positive

Epitope: AHYLQWNGNPLS
Chemical type: Peptide from protein
Sequence: AHYLQWNGNPLS
Position: 832-843
Source molecule (GenBank): F1KUR2.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Vit6_1
Chemical type: Peptide from protein
Sequence: RYLYKGQISNGLSKL
Position: 40-54
Source molecule (GenBank): F1KQS3.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Vit6_1
Chemical type: Peptide from protein
Sequence: RYLYKGQISNGLSKL
Position: 40-54
Source molecule (GenBank): F1KQS3.1
Assay category: MhcLigandElution
Result: Positive