Reference: Xiaoxiao Jin; Yan Ding; Shihui Sun; Xinyi Wang; Zining Zhou; Xiaotao Liu; Miaomiao Li; Xian Chen; Anran Shen; Yandan Wu; Bicheng Liu; Jianqiong Zhang; Jian Li; Yi Yang; Haibo Qiu; Chuanlai Shen; Yuxian He; Guangyu Zhao. "Screening HLA-A-restricted T cell epitopes of SARS-CoV-2 and the induction of CD8<sup>+</sup> T cell responses in HLA-A transgenic mice." Cellular & molecular immunology (2021).

Epitope: A6
Chemical type: Peptide from protein
Sequence: SVLLFLAFV
Position: 16-24
Source molecule (GenBank): P0DTC4.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: A6
Chemical type: Peptide from protein
Sequence: SVLLFLAFV
Position: 16-24
Source molecule (GenBank): P0DTC4.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: A6
Chemical type: Peptide from protein
Sequence: SVLLFLAFV
Position: 16-24
Source molecule (GenBank): P0DTC4.1
Assay category: MhcBinding
Result: Positive-High

Epitope: A6
Chemical type: Peptide from protein
Sequence: SVLLFLAFV
Position: 16-24
Source molecule (GenBank): P0DTC4.1
Assay category: MhcBinding
Result: Negative

Epitope: A12
Chemical type: Peptide from protein
Sequence: NIVNVSLVK
Position: 45-53
Source molecule (GenBank): P0DTC4.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: A12
Chemical type: Peptide from protein
Sequence: NIVNVSLVK
Position: 45-53
Source molecule (GenBank): P0DTC4.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: A12
Chemical type: Peptide from protein
Sequence: NIVNVSLVK
Position: 45-53
Source molecule (GenBank): P0DTC4.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: A12
Chemical type: Peptide from protein
Sequence: NIVNVSLVK
Position: 45-53
Source molecule (GenBank): P0DTC4.1
Assay category: MhcBinding
Result: Negative

Epitope: A12
Chemical type: Peptide from protein
Sequence: NIVNVSLVK
Position: 45-53
Source molecule (GenBank): P0DTC4.1
Assay category: MhcBinding
Result: Negative

Epitope: A18
Chemical type: Peptide from protein
Sequence: RVKNLNSSR
Position: 61-69
Source molecule (GenBank): P0DTC4.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: A18
Chemical type: Peptide from protein
Sequence: RVKNLNSSR
Position: 61-69
Source molecule (GenBank): P0DTC4.1
Assay category: MhcBinding
Result: Positive-Low

Epitope: A18
Chemical type: Peptide from protein
Sequence: RVKNLNSSR
Position: 61-69
Source molecule (GenBank): P0DTC4.1
Assay category: MhcBinding
Result: Negative

Epitope: A18
Chemical type: Peptide from protein
Sequence: RVKNLNSSR
Position: 61-69
Source molecule (GenBank): P0DTC4.1
Assay category: MhcBinding
Result: Negative

Epitope: A21
Chemical type: Peptide from protein
Sequence: VLLFLAFVVF
Position: 17-26
Source molecule (GenBank): P0DTC4.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: A21
Chemical type: Peptide from protein
Sequence: VLLFLAFVVF
Position: 17-26
Source molecule (GenBank): P0DTC4.1
Assay category: MhcBinding
Result: Negative

Epitope: A22
Chemical type: Peptide from protein
Sequence: TLIVNSVLLF
Position: 11-20
Source molecule (GenBank): P0DTC4.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: A22
Chemical type: Peptide from protein
Sequence: TLIVNSVLLF
Position: 11-20
Source molecule (GenBank): P0DTC4.1
Assay category: MhcBinding
Result: Negative

Epitope: B5
Chemical type: Peptide from protein
Sequence: LIFLWLLWPV
Position: 51-60
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: B6
Chemical type: Peptide from protein
Sequence: TLACFVLAAV
Position: 61-70
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: B6
Chemical type: Peptide from protein
Sequence: TLACFVLAAV
Position: 61-70
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: B6
Chemical type: Peptide from protein
Sequence: TLACFVLAAV
Position: 61-70
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Positive
Host: F 10 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: B6
Chemical type: Peptide from protein
Sequence: TLACFVLAAV
Position: 61-70
Source molecule (GenBank): P0DTC5.1
Assay category: MhcBinding
Result: Positive-High

Epitope: B6
Chemical type: Peptide from protein
Sequence: TLACFVLAAV
Position: 61-70
Source molecule (GenBank): P0DTC5.1
Assay category: MhcBinding
Result: Positive-High

Epitope: B6
Chemical type: Peptide from protein
Sequence: TLACFVLAAV
Position: 61-70
Source molecule (GenBank): P0DTC5.1
Assay category: MhcBinding
Result: Positive-Low

Epitope: B6
Chemical type: Peptide from protein
Sequence: TLACFVLAAV
Position: 61-70
Source molecule (GenBank): P0DTC5.1
Assay category: MhcBinding
Result: Positive-Intermediate

Epitope: B9
Chemical type: Peptide from protein
Sequence: LLWPVTLAC
Position: 56-64
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: B12
Chemical type: Peptide from protein
Sequence: VTLACFVLA
Position: 60-68
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: B12
Chemical type: Peptide from protein
Sequence: VTLACFVLA
Position: 60-68
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: B12
Chemical type: Peptide from protein
Sequence: VTLACFVLA
Position: 60-68
Source molecule (GenBank): P0DTC5.1
Assay category: MhcBinding
Result: Positive-Low

Epitope: B14
Chemical type: Peptide from protein
Sequence: YIIKLIFLWL
Position: 47-56
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: B28
Chemical type: Peptide from protein
Sequence: LYIIKLIFLW
Position: 46-55
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: B28
Chemical type: Peptide from protein
Sequence: LYIIKLIFLW
Position: 46-55
Source molecule (GenBank): P0DTC5.1
Assay category: MhcBinding
Result: Negative

Epitope: B35
Chemical type: Peptide from protein
Sequence: YYKLGASQR
Position: 178-186
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: B35
Chemical type: Peptide from protein
Sequence: YYKLGASQR
Position: 178-186
Source molecule (GenBank): P0DTC5.1
Assay category: MhcBinding
Result: Positive-Low

Epitope: C4
Chemical type: Peptide from protein
Sequence: YLGTGPEAGL
Position: 112-121
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: C10
Chemical type: Peptide from protein
Sequence: QTVTLLPAA
Position: 390-398
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: C10
Chemical type: Peptide from protein
Sequence: QTVTLLPAA
Position: 390-398
Source molecule (GenBank): P0DTC9.1
Assay category: MhcBinding
Result: Positive-Low

Epitope: C14
Chemical type: Peptide from protein
Sequence: RTATKAYNV
Position: 262-270
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: C23
Chemical type: Peptide from protein
Sequence: FTALTQHGK
Position: 53-61
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: C41
Chemical type: Peptide from protein
Sequence: SSRNSTPGS
Position: 193-201
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: C44
Chemical type: Peptide from protein
Sequence: SSRSRNSSR
Position: 187-195
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: D6
Chemical type: Peptide from protein
Sequence: RLDKVEAEV
Position: 983-991
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: D6
Chemical type: Peptide from protein
Sequence: RLDKVEAEV
Position: 983-991
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Host: F 10 weeks
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: D6
Chemical type: Peptide from protein
Sequence: RLDKVEAEV
Position: 983-991
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: D6
Chemical type: Peptide from protein
Sequence: RLDKVEAEV
Position: 983-991
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive-High

Epitope: D6
Chemical type: Peptide from protein
Sequence: RLDKVEAEV
Position: 983-991
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Negative

Epitope: D6
Chemical type: Peptide from protein
Sequence: RLDKVEAEV
Position: 983-991
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive-Low

Epitope: D27
Chemical type: Peptide from protein
Sequence: NTQEVFAQV
Position: 777-785
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: D28
Chemical type: Peptide from protein
Sequence: TQLNRALTGI
Position: 761-770
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: D35
Chemical type: Peptide from protein
Sequence: MTSCCSCLK
Position: 1237-1245
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: D36
Chemical type: Peptide from protein
Sequence: RLFRKSNLK
Position: 454-462
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: D39
Chemical type: Peptide from protein
Sequence: VTLADAGFIK
Position: 826-835
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: D50
Chemical type: Peptide from protein
Sequence: GVYYHKNNK
Position: 142-150
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: D50
Chemical type: Peptide from protein
Sequence: GVYYHKNNK
Position: 142-150
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive-Low

Epitope: D51
Chemical type: Peptide from protein
Sequence: QYIKWPWYI
Position: 1208-1216
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: D57
Chemical type: Peptide from protein
Sequence: VYSSANNCTF
Position: 159-168
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: D75
Chemical type: Peptide from protein
Sequence: HVSGTNGTK
Position: 69-77
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: D78
Chemical type: Peptide from protein
Sequence: QTNSPRRAR
Position: 677-685
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: D78
Chemical type: Peptide from protein
Sequence: QTNSPRRAR
Position: 677-685
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Negative

Epitope: D78
Chemical type: Peptide from protein
Sequence: QTNSPRRAR
Position: 677-685
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Negative

Epitope: D78
Chemical type: Peptide from protein
Sequence: QTNSPRRAR
Position: 677-685
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Negative

Epitope: D79
Chemical type: Peptide from protein
Sequence: NVYADSFVIR
Position: 394-403
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: D79
Chemical type: Peptide from protein
Sequence: NVYADSFVIR
Position: 394-403
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Negative

Epitope: D79
Chemical type: Peptide from protein
Sequence: NVYADSFVIR
Position: 394-403
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Positive-Intermediate

Epitope: D79
Chemical type: Peptide from protein
Sequence: NVYADSFVIR
Position: 394-403
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Negative

Epitope: D79
Chemical type: Peptide from protein
Sequence: NVYADSFVIR
Position: 394-403
Source molecule (GenBank): P0DTC2.1
Assay category: MhcBinding
Result: Negative

Epitope: R1
Chemical type: Peptide from protein
Sequence: LLMPILTLT
Position: 4632-4640
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: R23
Chemical type: Peptide from protein
Sequence: LSFKELLVYA
Position: 4758-4767
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: R23
Chemical type: Peptide from protein
Sequence: LSFKELLVYA
Position: 4758-4767
Source molecule (GenBank): P0DTD1.1
Assay category: MhcBinding
Result: Positive-Intermediate

Epitope: R27
Chemical type: Peptide from protein
Sequence: AMYTPHTVL
Position: 5315-5323
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: R34
Chemical type: Peptide from protein
Sequence: MTNRQFHQK
Position: 4958-4966
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: R34
Chemical type: Peptide from protein
Sequence: MTNRQFHQK
Position: 4958-4966
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive-Low
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: R34
Chemical type: Peptide from protein
Sequence: MTNRQFHQK
Position: 4958-4966
Source molecule (GenBank): P0DTD1.1
Assay category: MhcBinding
Result: Negative

Epitope: R34
Chemical type: Peptide from protein
Sequence: MTNRQFHQK
Position: 4958-4966
Source molecule (GenBank): P0DTD1.1
Assay category: MhcBinding
Result: Positive-High

Epitope: R34
Chemical type: Peptide from protein
Sequence: MTNRQFHQK
Position: 4958-4966
Source molecule (GenBank): P0DTD1.1
Assay category: MhcBinding
Result: Positive-Intermediate

Epitope: R55
Chemical type: Peptide from protein
Sequence: NFNKDFYDF
Position: 4806-4814
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo