Reference: Sebastian Spindeldreher; Anette Karle; Evelyne Correia; Maxime Tenon; Sascha Gottlieb; Thomas Huber; Bernard Maillere; Frank Kolbinger. "T cell epitope mapping of secukinumab and ixekizumab in healthy donors." mAbs (2020).

Epitope: Secukinumab/ixekizumab light P 48
Chemical type: Peptide from protein
Sequence: ADYEKHKVYACEVTHQG
Position: 185-201
Source molecule (GenBank): BAF64541.1
Assay category: MhcLigandElution
Result: Positive

Epitope: IXE40
Chemical type: Peptide from protein
Sequence: AEDVGVYYCSQSTHLPFT
Position: 229-246
Source molecule (GenBank): 6NOU_A
Assay category: TCell
Result: Positive
MHC allele types: 354 425
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Cell Line / Clone

Epitope: IXE40
Chemical type: Peptide from protein
Sequence: AEDVGVYYCSQSTHLPFT
Position: 229-246
Source molecule (GenBank): 6NOU_A
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab/ixekizumab P 112
Chemical type: Peptide from protein
Sequence: AKVQWKVDNALQSGNSQESVTEQDSKD
Position: 145-171
Source molecule (GenBank): BAF64541.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab light P 29
Chemical type: Peptide from protein
Sequence: ALQSGNSQESVTEQDSKD
Position: 154-171
Source molecule (GenBank): BAF64541.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab heavy P 109
Chemical type: Peptide from protein
Sequence: APIEKTISKAKGQPR
Position: 339-353
Source molecule (GenBank): ARA90391.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Ixekizumab heavy P 50
Chemical type: Peptide from protein
Sequence: DGSFFLYSRLTVDKSR
Position: 316-331
Source molecule (GenBank): 10334595
Assay category: MhcLigandElution
Result: Positive

Epitope: Ixekizumab light P 139
Chemical type: Peptide from protein
Sequence: DIVMTQTPLSLSVTPGQPASISCRSSRSLVH
Position: 1-31
Source molecule (GenBank): 6NOV_B
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab heavy P 53
Chemical type: Peptide from protein
Sequence: DNAKNSLYLQMNSLRVEDT
Position: 57-75
Source molecule (GenBank): 815084992
Assay category: MhcLigandElution
Result: Positive

Epitope: Ixekizumab light P 152
Chemical type: Peptide from protein
Sequence: DVGVYYCSQSTHL
Position: 87-99
Source molecule (GenBank): 6NOV_B
Assay category: MhcLigandElution
Result: Positive

Epitope: Ixekizumab light P 150
Chemical type: Peptide from protein
Sequence: EDVGVYYCSQSTHLP
Position: 86-100
Source molecule (GenBank): 6NOV_B
Assay category: MhcLigandElution
Result: Positive

Epitope: Ixekizumab heavy P 58
Chemical type: Peptide from protein
Sequence: EGNVFSCSVMHEALHN
Position: 86-101
Source molecule (GenBank): 223060
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab light P 6
Chemical type: Peptide from protein
Sequence: EIVLTQSPGTLS
Position: 1-12
Source molecule (GenBank): BAF64541.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab light P 8
Chemical type: Peptide from protein
Sequence: EIVLTQSPGTLSLSPG
Position: 1-16
Source molecule (GenBank): BAF64541.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab light P 62
Chemical type: Peptide from protein
Sequence: EIVLTQSPGTLSLSPGERAT
Position: 1-20
Source molecule (GenBank): BAF64541.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab light P 58
Chemical type: Peptide from protein
Sequence: EIVLTQSPGTLSLSPGERATLSCRASQSV
Position: 1-29
Source molecule (GenBank): BAF64541.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab light P 114
Chemical type: Peptide from protein
Sequence: EIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLA
Position: 1-35
Source molecule (GenBank): BAF64541.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab heavy P 38
Chemical type: Peptide from protein
Sequence: EVQLVESGGGLVQ
Position: 1-13
Source molecule (GenBank): ARA90391.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab/ixekizumab heavy P 69
Chemical type: Peptide from protein
Sequence: GPSVFLFPPKPKD
Position: 246-258
Source molecule (GenBank): ARA90391.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab/ixekizumab heavy P 58
Chemical type: Peptide from protein
Sequence: GQPENNYKTTPPVLDSD
Position: 394-410
Source molecule (GenBank): ARA90391.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab heavy P 97
Chemical type: Peptide from protein
Sequence: GSFFLYSKLTVDKSRWQQG
Position: 411-429
Source molecule (GenBank): ARA90391.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Ixekizumab light P 89
Chemical type: Peptide from protein
Sequence: GSGSGTDFTLKISRVEAED
Position: 69-87
Source molecule (GenBank): 6NOV_B
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab heavy P 51
Chemical type: Peptide from protein
Sequence: GTQTYICNVNHKPS
Position: 203-216
Source molecule (GenBank): ARA90391.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab heavy P 68
Chemical type: Peptide from protein
Sequence: HEALHNHYTQKSLSLSPGK
Position: 438-456
Source molecule (GenBank): ARA90391.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Ixekizumab light P 144
Chemical type: Peptide from protein
Sequence: HKVYACEVTHQGLS
Position: 194-207
Source molecule (GenBank): 6NOV_B
Assay category: MhcLigandElution
Result: Positive

Epitope: Ixekizumab heavy P 55
Chemical type: Peptide from protein
Sequence: IEKTISKAKGQPREPQ
Position: 354-369
Source molecule (GenBank): QBK47431.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab/ixekizumab light P 34
Chemical type: Peptide from protein
Sequence: KDSTYSLSSTLTLSK
Position: 170-184
Source molecule (GenBank): BAF64541.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Ixekizumab light P 108
Chemical type: Peptide from protein
Sequence: KHKVYACEVTHQG
Position: 193-205
Source molecule (GenBank): 6NOV_B
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab/ixekizumab light P 32
Chemical type: Peptide from protein
Sequence: KHKVYACEVTHQGLS
Position: 189-203
Source molecule (GenBank): BAF64541.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab heavy P 63
Chemical type: Peptide from protein
Sequence: KNSLYLQMNSLRVED
Position: 62-76
Source molecule (GenBank): 4456588
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab light P 80
Chemical type: Peptide from protein
Sequence: KPGQAPRLLIYG
Position: 40-51
Source molecule (GenBank): BAF64541.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Ixekizumab light P 179
Chemical type: Peptide from protein
Sequence: KVDNALQSGNSQESVTEQDSKDSTYSLSSTL
Position: 154-184
Source molecule (GenBank): 6NOV_B
Assay category: MhcLigandElution
Result: Positive

Epitope: Ixekizumab light P 35
Chemical type: Peptide from protein
Sequence: LLIYKVSNRFIG
Position: 51-62
Source molecule (GenBank): 6NOV_B
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab/ixekizumab light P 27
Chemical type: Peptide from protein
Sequence: LQSGNSQESVTEQDSKD
Position: 155-171
Source molecule (GenBank): BAF64541.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab light P 106
Chemical type: Peptide from protein
Sequence: NALQSGNSQESVTE
Position: 153-166
Source molecule (GenBank): BAF64541.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab/ixekizumab light P 117
Chemical type: Peptide from protein
Sequence: NFYPREAKVQ
Position: 139-148
Source molecule (GenBank): BAF64541.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Ixekizumab light P 121
Chemical type: Peptide from protein
Sequence: NTYLHWYLQKPGQSPQ
Position: 35-50
Source molecule (GenBank): 6NOV_B
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab heavy P 102
Chemical type: Peptide from protein
Sequence: PEVKFNWYVDGVEVHNAK
Position: 280-297
Source molecule (GenBank): ARA90391.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab heavy P 118
Chemical type: Peptide from protein
Sequence: PEVKFNWYVDGVEVHNAKTKPREEQ
Position: 280-304
Source molecule (GenBank): ARA90391.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Ixekizumab heavy P 100
Chemical type: Peptide from protein
Sequence: PEVQFNWYVDGVEVHNAKTKPR
Position: 150-171
Source molecule (GenBank): 1463807526
Assay category: MhcLigandElution
Result: Positive

Epitope: Ixekizumab heavy P 103
Chemical type: Peptide from protein
Sequence: PEVQFNWYVDGVEVHNAKTKPREEQF
Position: 150-175
Source molecule (GenBank): 1463807526
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab heavy P 72
Chemical type: Peptide from protein
Sequence: RGTLVTVSSASTKGPSVFPLAPSSK
Position: 118-142
Source molecule (GenBank): 323432279
Assay category: MhcLigandElution
Result: Positive

Epitope: Ixekizumab heavy P 80
Chemical type: Peptide from protein
Sequence: SDGSFFLYSRLTVDKSRWQEG
Position: 315-335
Source molecule (GenBank): 10334595
Assay category: MhcLigandElution
Result: Positive

Epitope: Ixekizumab light P 100
Chemical type: Peptide from protein
Sequence: SKDSTYSLSSTLTLSK
Position: 173-188
Source molecule (GenBank): 6NOV_B
Assay category: MhcLigandElution
Result: Positive

Epitope: Ixekizumab light P 63
Chemical type: Peptide from protein
Sequence: SPQLLIYKVSNRFI
Position: 48-61
Source molecule (GenBank): 6NOV_B
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab light P 119
Chemical type: Peptide from protein
Sequence: SPVTKSFNRGEC
Position: 204-215
Source molecule (GenBank): BAF64541.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab/ixekizumab light P 16
Chemical type: Peptide from protein
Sequence: SQESVTEQDSKD
Position: 160-171
Source molecule (GenBank): BAF64541.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Ixekizumab heavy P 113
Chemical type: Peptide from protein
Sequence: STYRVVSVLTVLHQD
Position: 320-334
Source molecule (GenBank): QBK47431.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab/ixekizumab light P 28
Chemical type: Peptide from protein
Sequence: STYSLSSTLTLSKAD
Position: 172-186
Source molecule (GenBank): BAF64541.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab/ixekizumab light P 109
Chemical type: Peptide from protein
Sequence: STYSLSSTLTLSKADYEKH
Position: 172-190
Source molecule (GenBank): BAF64541.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Ixekizumab light P 40
Chemical type: Peptide from protein
Sequence: TPGQPASISCRSSR
Position: 14-27
Source molecule (GenBank): 6NOV_B
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab/ixekizumab heavy P 148
Chemical type: Peptide from protein
Sequence: TSGVHTFPAVLQSSG
Position: 173-187
Source molecule (GenBank): ARA90391.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Ixekizumab light P 71
Chemical type: Peptide from protein
Sequence: TYLHWYLQKPGQSPQ
Position: 36-50
Source molecule (GenBank): 6NOV_B
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab heavy P 85
Chemical type: Peptide from protein
Sequence: VAAINQDGSEKYYVGS
Assay category: MhcLigandElution
Result: Positive

Epitope: Secukinumab heavy P 94
Chemical type: Peptide from protein
Sequence: VAAINQDGSEKYYVGSV
Assay category: MhcLigandElution
Result: Positive

Epitope: Ixekizumab heavy P 69
Chemical type: Peptide from protein
Sequence: VRQAPGQGLE
Position: 56-65
Source molecule (GenBank): QBK47431.1
Assay category: MhcLigandElution
Result: Positive

Epitope: Ixekizumab light P 170
Chemical type: Peptide from protein
Sequence: VTHQGLSSPVTKSFNRGEC
Position: 201-219
Source molecule (GenBank): 6NOV_B
Assay category: MhcLigandElution
Result: Positive