Reference: Yu Zhao; Chengtao He; Min Peng; Min Li; Xiaotao Liu; Xuelian Han; Qiang Fu; Yandan Wu; Fangping Yue; Chunguang Yan; Guangyu Zhao; Chuanlai Shen. "Large-Scale Screening of CD4<sup>+</sup> T-Cell Epitopes From SARS-CoV-2 Proteins and the Universal Detection of SARS-CoV-2 Specific T Cells for Northeast Asian Population." Journal of medical virology (2025).

Epitope: Z31
Chemical type: Peptide from protein
Sequence: AVYRINWITGGIAIAM
Position: 69-84
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Positive
MHC allele types: 409 406
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Z31
Chemical type: Peptide from protein
Sequence: AVYRINWITGGIAIAM
Position: 69-84
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Z31
Chemical type: Peptide from protein
Sequence: AVYRINWITGGIAIAM
Position: 69-84
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Z31
Chemical type: Peptide from protein
Sequence: AVYRINWITGGIAIAM
Position: 69-84
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Z53
Chemical type: Peptide from protein
Sequence: CLLQFAYANRNRFLYI
Position: 33-48
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Positive
MHC allele types: 755 419 425 436 409 419 408 417
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Z53
Chemical type: Peptide from protein
Sequence: CLLQFAYANRNRFLYI
Position: 33-48
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Z99
Chemical type: Peptide from protein
Sequence: DLPKEITVATSRTLSY
Position: 163-178
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Positive
MHC allele types: 57 417
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Z99
Chemical type: Peptide from protein
Sequence: DLPKEITVATSRTLSY
Position: 163-178
Source molecule (GenBank): P0DTC5.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Z242
Chemical type: Peptide from protein
Sequence: HWPQIAQFAPSASAFF
Position: 300-315
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
MHC allele types: 409 406
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Z595
Chemical type: Peptide from protein
Sequence: PQIAQFAPSASAFFGM
Position: 302-317
Source molecule (GenBank): P0DTC9.1
Assay category: TCell
Result: Positive
MHC allele types: 413 750
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Z156
Chemical type: Peptide from protein
Sequence: FHQKLLKSIAATRGAT
Position: 4963-4978
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
MHC allele types: 755 419
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Z438
Chemical type: Peptide from protein
Sequence: LVRKIFVDGVPFVVST
Position: 4721-4736
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
MHC allele types: 57 417
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Z451
Chemical type: Peptide from protein
Sequence: MNLKYAISAKNRARTV
Position: 4934-4949
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
MHC allele types: 419
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Z92
Chemical type: Peptide from protein
Sequence: DGVPFVVSTGYHFREL
Position: 4728-4743
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Negative
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Z92
Chemical type: Peptide from protein
Sequence: DGVPFVVSTGYHFREL
Position: 4728-4743
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Z92
Chemical type: Peptide from protein
Sequence: DGVPFVVSTGYHFREL
Position: 4728-4743
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
MHC allele types: 52 406
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Z92
Chemical type: Peptide from protein
Sequence: DGVPFVVSTGYHFREL
Position: 4728-4743
Source molecule (GenBank): P0DTD1.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Z1012
Chemical type: Peptide from protein
Sequence: IAYTMSLGAENSVAYS
Position: 693-708
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
MHC allele types: 417 409 409 417
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: Z885
Chemical type: Peptide from protein
Sequence: VNFNFNGLTGTGVLTE
Position: 539-554
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD4+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Z885
Chemical type: Peptide from protein
Sequence: VNFNFNGLTGTGVLTE
Position: 539-554
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: spleen
Culture conditions: Direct Ex Vivo

Epitope: Z885
Chemical type: Peptide from protein
Sequence: VNFNFNGLTGTGVLTE
Position: 539-554
Source molecule (GenBank): P0DTC2.1
Assay category: TCell
Result: Positive
MHC allele types: 417 356 417 409
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated