Reference: Jessica S W Borgers; Divya Lenkala; Victoria Kohler; Emily K Jackson; Matthijs D Linssen; Sebastian Hymson; Brian McCarthy; Elizabeth O'Reilly Cosgrove; Kristen N Balogh; Ekaterina Esaulova; Kimberly Starr; Yvonne Ware; Sebastian Klobuch; Tracey Sciuto; Xi Chen; Gauri Mahimkar; Joong Hyuk F Sheen; Suchitra Ramesh; Sofie Wilgenhof; Johannes V van Thienen; Karina C Scheiner; Inge Jedema; Michael Rooney; Jesse Z Dong; John R Srouji; Vikram R Juneja; Christina M Arieta; Bastiaan Nuijen; Claudia Gottstein; Olivia C Finney; Kelledy Manson; Cynthia M Nijenhuis; Richard B Gaynor; Mark DeMario; John B Haanen; Marit M van Buuren. "Personalized, autologous neoantigen-specific T cell therapy in metastatic melanoma: a phase 1 trial." Nature medicine (2025).

Epitope: HSPG2 4003:R>Q
Chemical type: Peptide, no natural source
Sequence: QSAEPLAL
Assay category: TCell
Result: Positive
Host: F 56 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HSPG2 4003:R>Q
Chemical type: Peptide, no natural source
Sequence: QSAEPLAL
Assay category: TCell
Result: Positive
Host: F 56 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: HSPG2 4003:R>Q
Chemical type: Peptide, no natural source
Sequence: QSAEPLAL
Assay category: TCell
Result: Positive
Host: F 56 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HSPG2 4003:R>Q
Chemical type: Peptide, no natural source
Sequence: QSAEPLAL
Assay category: TCell
Result: Positive
Host: F 56 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: HSPG2 4003:R>Q
Chemical type: Peptide, no natural source
Sequence: QSAEPLAL
Assay category: TCell
Result: Negative
Host: F 56 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GLG1 522:D>E
Chemical type: Peptide, no natural source
Sequence: TACKHIRSGEPMILSCLMEHLYTEK
Assay category: TCell
Result: Positive
Host: F 46 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GLG1 522:D>E
Chemical type: Peptide, no natural source
Sequence: TACKHIRSGEPMILSCLMEHLYTEK
Assay category: TCell
Result: Positive
Host: F 46 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GLG1 522:D>E
Chemical type: Peptide, no natural source
Sequence: TACKHIRSGEPMILSCLMEHLYTEK
Assay category: TCell
Result: Positive
Host: F 46 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GLG1 522:D>E
Chemical type: Peptide, no natural source
Sequence: TACKHIRSGEPMILSCLMEHLYTEK
Assay category: TCell
Result: Positive
Host: F 46 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GLG1 522:D>E
Chemical type: Peptide, no natural source
Sequence: TACKHIRSGEPMILSCLMEHLYTEK
Assay category: TCell
Result: Negative
Host: F 46 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: GLG1 522:D>E
Chemical type: Peptide, no natural source
Sequence: TACKHIRSGEPMILSCLMEHLYTEK
Assay category: TCell
Result: Positive
Host: F 46 years
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: DYNC2H1 2720:P>S
Chemical type: Peptide, no natural source
Sequence: DYQFVHSTF
Assay category: TCell
Result: Positive
Host: F 72 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: DYNC2H1 2720:P>S
Chemical type: Peptide, no natural source
Sequence: DYQFVHSTF
Assay category: TCell
Result: Positive
Host: F 72 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: DYNC2H1 2720:P>S
Chemical type: Peptide, no natural source
Sequence: DYQFVHSTF
Assay category: TCell
Result: Positive
Host: F 72 years
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: DYNC2H1 2720:P>S
Chemical type: Peptide, no natural source
Sequence: DYQFVHSTF
Assay category: TCell
Result: Positive
Host: F 72 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: DYNC2H1 2720:P>S
Chemical type: Peptide, no natural source
Sequence: DYQFVHSTF
Assay category: TCell
Result: Positive
Host: F 72 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: DYNC2H1 2720:P>S
Chemical type: Peptide, no natural source
Sequence: DYQFVHSTF
Assay category: TCell
Result: Positive
Host: F 72 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: ATM 337:R>C
Chemical type: Peptide, no natural source
Sequence: HIGSRGKYSSGFCNIAVKENLIELM
Assay category: TCell
Result: Positive
Host: F 72 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: ATM 337:R>C
Chemical type: Peptide, no natural source
Sequence: HIGSRGKYSSGFCNIAVKENLIELM
Assay category: TCell
Result: Positive
Host: F 72 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: ATM 337:R>C
Chemical type: Peptide, no natural source
Sequence: HIGSRGKYSSGFCNIAVKENLIELM
Assay category: TCell
Result: Positive
Host: F 72 years
Cell type: T cell CD4+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: AP2M1 46:P>L
Chemical type: Peptide, no natural source
Sequence: RSLVTNIAR
Assay category: TCell
Result: Negative
Host: F 56 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: SLC35B1 15:P>L
Chemical type: Peptide, no natural source
Sequence: LVPDRLRLLL
Assay category: TCell
Result: Negative
Host: F 56 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: ZNF536 491:C>S
Chemical type: Peptide, no natural source
Sequence: SLNLVPPLK
Assay category: TCell
Result: Negative
Host: F 56 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: LIMA1 401:E>A
Chemical type: Peptide, no natural source
Sequence: TVYPMARLL
Assay category: TCell
Result: Positive
Host: F 56 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: LIMA1 401:E>A
Chemical type: Peptide, no natural source
Sequence: TVYPMARLL
Assay category: TCell
Result: Positive
Host: F 56 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: LIMA1 401:E>A
Chemical type: Peptide, no natural source
Sequence: TVYPMARLL
Assay category: TCell
Result: Positive
Host: F 56 years
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: COL27A1 670:G>A 2
Chemical type: Peptide, no natural source
Sequence: LPGPPAAK
Assay category: TCell
Result: Negative
Host: M 57 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: ENTPD4 99:G>A
Chemical type: Peptide, no natural source
Sequence: ASRVFVYCW
Assay category: TCell
Result: Negative
Host: M 57 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: H3F3B 31:P>S 2
Chemical type: Peptide, no natural source
Sequence: KSASSTGGVKK
Assay category: TCell
Result: Negative
Host: M 57 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: SNRPB 217:P>S
Chemical type: Peptide, no natural source
Sequence: TPMGMPSPGM
Assay category: TCell
Result: Negative
Host: M 57 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: LDLR 808:L>F
Chemical type: Peptide, no natural source
Sequence: FLWKNWRL
Assay category: TCell
Result: Negative
Host: F 46 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: MKRN2 309:N>K
Chemical type: Peptide, no natural source
Sequence: DQKKKNEL
Assay category: TCell
Result: Negative
Host: F 46 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: MYO1C 121:F>L 2
Chemical type: Peptide, no natural source
Sequence: EATKRLLQL
Assay category: TCell
Result: Negative
Host: F 46 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: LDHB 142:D>N 2
Chemical type: Peptide, no natural source
Sequence: VSNPVNILTY
Assay category: TCell
Result: Negative
Host: F 72 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: LDHB 142:D>N 3
Chemical type: Peptide, no natural source
Sequence: SNPVNILTY
Assay category: TCell
Result: Negative
Host: F 72 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: MKRN1 412:R>W
Chemical type: Peptide, no natural source
Sequence: RYWAQRRNHFW
Assay category: TCell
Result: Negative
Host: F 72 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: DYRK2 268:R>G
Chemical type: Peptide, no natural source
Sequence: EEIGILEH
Assay category: TCell
Result: Positive
Host: F 72 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: DYRK2 268:R>G
Chemical type: Peptide, no natural source
Sequence: EEIGILEH
Assay category: TCell
Result: Negative
Host: F 72 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: DYRK2 268:R>G
Chemical type: Peptide, no natural source
Sequence: EEIGILEH
Assay category: TCell
Result: Positive
Host: F 72 years
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: BATF2 18 fs1
Chemical type: Peptide, no natural source
Sequence: RAYPSSTSLWK
Assay category: TCell
Result: Negative
Host: F 58 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: MICAL2 647:S>L
Chemical type: Peptide, no natural source
Sequence: NADLSLAKL
Assay category: TCell
Result: Negative
Host: F 58 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: SLIT2 402:S>F
Chemical type: Peptide, no natural source
Sequence: FLYDNKLQT
Assay category: TCell
Result: Negative
Host: F 58 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: ZMYM2 633:S>N
Chemical type: Peptide, no natural source
Sequence: FVAPNDIQL
Assay category: TCell
Result: Negative
Host: F 58 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: AKAP12 923:E>K
Chemical type: Peptide, no natural source
Sequence: SVTEPLKQV
Assay category: TCell
Result: Negative
Host: M 32 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: GLCCI1 172:S>F 3
Chemical type: Peptide, no natural source
Sequence: FLDTITGPYLT
Assay category: TCell
Result: Negative
Host: M 32 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: WASF2 116:L>F
Chemical type: Peptide, no natural source
Sequence: FPVPVLETY
Assay category: TCell
Result: Positive
Host: M 32 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: WASF2 116:L>F
Chemical type: Peptide, no natural source
Sequence: FPVPVLETY
Assay category: TCell
Result: Positive
Host: M 32 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: WASF2 116:L>F
Chemical type: Peptide, no natural source
Sequence: FPVPVLETY
Assay category: TCell
Result: Positive
Host: M 32 years
Cell type: Jurkat cell (T cell)
Tissue: blood
Culture conditions: Engineered

Epitope: SMARCA5 297:F>L 2
Chemical type: Peptide, no natural source
Sequence: LIKEKSVLKK
Assay category: TCell
Result: Negative
Host: F 70 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: SPTLC3 54:S>L
Chemical type: Peptide, no natural source
Sequence: FYDKLIVEL
Assay category: TCell
Result: Negative
Host: F 70 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: EHBP1L1 209:P>L
Chemical type: Peptide, no natural source
Sequence: APEARARVL
Assay category: TCell
Result: Negative
Host: F 64 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: FKBP9 113:P>L 1
Chemical type: Peptide, no natural source
Sequence: RRFVKIPLK
Assay category: TCell
Result: Negative
Host: F 39 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: NUP35 187:S>F
Chemical type: Peptide, no natural source
Sequence: SAFYILLQF
Assay category: TCell
Result: Negative
Host: F 39 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: SMARCC2 303:P>L 1
Chemical type: Peptide, no natural source
Sequence: LSPSPTPEAK
Assay category: TCell
Result: Negative
Host: F 39 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: TRIP12 290:P>S
Chemical type: Peptide, no natural source
Sequence: KSGPSGLQAK
Assay category: TCell
Result: Negative
Host: F 39 years
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: IFRG15 18:S>N 2
Chemical type: Peptide, no natural source
Sequence: QWFPNLELGHW
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: PLK4 797:P>S
Chemical type: Peptide, no natural source
Sequence: SASFFPIII
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: SLC39A1 106:T>M
Chemical type: Peptide, no natural source
Sequence: EALAALHVM
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: SLC3A2 521:S>F
Chemical type: Peptide, no natural source
Sequence: QSEDPGSLLFL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: ZFP82 21:E>K
Chemical type: Peptide, no natural source
Sequence: SIDFSPEEWKY
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: SAMD9 739:P>S 2
Chemical type: Peptide, no natural source
Sequence: YHHSGCGGTTL
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: SAMD9 739:P>S 2
Chemical type: Peptide, no natural source
Sequence: YHHSGCGGTTL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: CCDC57 328:P>S
Chemical type: Peptide, no natural source
Sequence: LPASVVLV
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: LOXL2 440:G>D
Chemical type: Peptide, no natural source
Sequence: RLNGDRNPY
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: NYNRIN 1086:T>I 3
Chemical type: Peptide, no natural source
Sequence: LIIPSNFTAL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: ZNF708 433:S>L
Chemical type: Peptide, no natural source
Sequence: FLILTKHKV
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: ZNF708 433:S>L
Chemical type: Peptide, no natural source
Sequence: FLILTKHKV
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo

Epitope: C11orf70 144:R>Q
Chemical type: Peptide, no natural source
Sequence: DELQRVLL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: MAP2 344:F>I
Chemical type: Peptide, no natural source
Sequence: ETSPFAPAIL
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: RNASEK 38:F>L
Chemical type: Peptide, no natural source
Sequence: APRRSPTPLFM
Assay category: TCell
Result: Positive
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Short Term Restimulated

Epitope: RNASEK 38:F>L
Chemical type: Peptide, no natural source
Sequence: APRRSPTPLFM
Assay category: TCell
Result: Negative
Cell type: T cell CD8+
Tissue: blood
Culture conditions: Direct Ex Vivo