Protein: Ribonuclease HI (Escherichia coli (strain K12))
UniProt: P0A7Y4
EC number: 3.1.26.4
Mutation: D134N
PDB: 2RN2
Experiment dataset: ProTherm
Method: Thermal
Measure: CD
pH: 3
Buffer: glycine-HCl 10 mM
Ion: NaCl 0.1 M
Tm: 49.7
dTm: -0.3
dHvH: 110.5
Reversibility: yes
Reference: Investigating the role of conserved residue Asp134 in Escherichia coli ribonuclease HI by site-directed random mutagenesis. — Haruki M|Oobatake M|Kanaya S|Noguchi E|Nakai C|Itaya M|Liu Y Y (Eur J Biochem;220;623-31, 1994)