Protein: Endolysin (Enterobacteria phage T4)
UniProt: P00720
EC number: 3.2.1.17
Mutation: M102A|M106A
PDB: 1L63|2LZM
Experiment dataset: ProTherm
Method: Thermal
Measure: CD, Fluorescence
pH: 5.4
Buffer: acetic acid, sodium acetate 1.4 mM, 8.6 mM
Temperature: 65.3
Ion: Sodium Chloride 0.10 M
ddG: 3.7
Reversibility: Yes(80%)
State: 2.0
Reference: Methionine and alanine substitutions show that the formation of wild-type-like structure in the carboxy-terminal domain of T4 lysozyme is a rate-limiting step in folding. — Lu J|Baase W A|Matthews B W|Gassner N C|Lindstrom J D|Dahlquist F W (Biochemistry;38;14451-60, 1999)