Protein: 2LSS ()
Mutation: D53Y|N65V
Experiment dataset: MegaScale
dG: 2.91063386417166
Trypsin: 0.7306499821775995
Chymotrypsin: -0.4399872023733637
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)