Protein: Ribonuclease HI (Escherichia coli (strain K12))
UniProt: P0A7Y4
EC number: 3.1.26.4
Mutation: I53D
PDB: 2RN2
Experiment dataset: ProTherm
Method: Urea
Measure: CD
pH: 5.5
Buffer: sodium acetate 20 mM
Temperature: 25
Ion: KCl 50 mM
dG: 5.6
ddG: 4.1
m-value: 2
Reversibility: Unknown
Reference: Destabilization of the Escherichia coli RNase H kinetic intermediate: switching between a two-state and three-state folding mechanism. — Spudich Giulietta M|Miller Erik J|Marqusee Susan (J Mol Biol;335;609-18, 2004)