Protein: 2K5P ()
Mutation: S25F
Experiment dataset: MegaScale
dG: 2.617392947394226
ddG: 0.09307073609548588
Trypsin: 1.0399849248786484
Chymotrypsin: -0.2107664031293732
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)