Protein: Thermonuclease (Staphylococcus aureus)
UniProt: P00644
EC number: 3.1.31.1
Mutation: V148M
PDB: 1STN
Experiment dataset: ProTherm
Method: GdnHCl
Measure: Fluorescence
pH: 8.8
Buffer: Tris-HCl 25 mM
Temperature: 20
Ion: NaCl 100 mM
dG: 5.1
ddG: 0.1
Cm: 0.88
m-value: 0.89
Reversibility: Unknown
Reference: In a staphylococcal nuclease mutant the side-chain of a lysine replacing valine 66 is fully buried in the hydrophobic core. — Shortle D|Gittis A G|Stites W E|Lattman E E (J Mol Biol;221;7-14, 1991)