Protein: Aspartate aminotransferase (Escherichia coli (strain K12))
UniProt: P00509
EC number: 2.6.1.1
Mutation: C181A
PDB: 1AMQ
Experiment dataset: ProTherm
Method: Urea
Measure: DSC
pH: 7.5
Buffer: potassium-HEPES 40 mM
Temperature: 25
dG: 8.32
ddG: 1.6
Cm: 3.53
m-value: 2.35
Reversibility: yes
Reference: Contribution to catalysis and stability of the five cysteines in Escherichia coli aspartate aminotransferase. Preparation and properties of a cysteine-free enzyme. — Planas A|Kirsch J F|Gloss L M (Biochemistry;31;32-9, 1992)