Protein: Ribonuclease (Bacillus amyloliquefaciens)
UniProt: P00648
EC number: 3.1.27.-
Mutation: Y60A|Y64A
PDB: 1BNI
Experiment dataset: ProTherm
Method: Urea
Measure: Fluorescence
pH: 6.3
Buffer: MES 50 micro M
Temperature: 25
ddG: 5
Reversibility: yes
Reference: The folding of an enzyme. III. Structure of the transition state for unfolding of barnase analysed by a protein engineering procedure. — Matouschek A|Serrano L|Fersht A R (J Mol Biol;224;805-18, 1992)