Protein: 2HBB ()
Mutation: I4D
Experiment dataset: MegaScale
Trypsin: -1.6035818290581152
Chymotrypsin: -2.619378817790787
Stabilizing: False
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)