Protein: Endolysin (Enterobacteria phage T4)
UniProt: P00720
EC number: 3.2.1.17
Mutation: L99G|E108V
PDB: 1L63|2LZM
Experiment dataset: ProTherm
Method: Thermal
Measure: CD
pH: 3
Buffer: Potassium phosphate 20 mM
Ion: KCl 25 mM
Tm: 30.4
dTm: -21.3
dHvH: 69
Reversibility: yes(>95%)
Reference: Structural analysis of a non-contiguous second-site revertant in T4 lysozyme shows that increasing the rigidity of a protein can enhance its stability. — Baase W A|Lindstrom J D|Poteete A R|Weaver L H|Wray J W (J Mol Biol;292;1111-20, 1999)