Protein: Ribonuclease (Bacillus amyloliquefaciens)
UniProt: P00648
EC number: 3.1.27.-
Mutation: K74A
PDB: 1BNI
Experiment dataset: ProTherm
Method: Urea
Measure: Fluorescence
pH: 6.3
Buffer: MES 50 mM
Temperature: 25
dG: 9.19
ddG: -0.36
Reversibility: yes
Reference: Effect of active site residues in barnase on activity and stability. — Serrano L|Fersht A R|Meiering E M (J Mol Biol;225;585-9, 1992)