Protein: 1PWT ()
Mutation: D25M
Experiment dataset: MegaScale
dG: 4.543962751539351
ddG: 0.34185817655068806
Trypsin: 1.997890802328124
Chymotrypsin: 0.8768485691723467
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)