Protein: Subtilisin BPN' (Bacillus amyloliquefaciens)
UniProt: P00782
EC number: 3.4.21.62
Mutation: T173M|G209D
PDB: 1SUP|1SPB
Experiment dataset: ProTherm
Method: Thermal
Measure: fluorescence
pH: 7.5
Buffer: Pipes 10 mM
Tm: 56.3
dTm: -0.5
dH: 72.78
State: 2.0
Reference: Engineering and directed evolution of a Ca2+ binding site A-deficient AprE mutant reveal an essential contribution of the loop Leu75-Leu82 to enzyme activity. — Romero-Garc?a Eliel R|T?llez-Valencia Alfredo|Trujillo Mar?a F|Sampedro Jos? G|N?jera Hugo|Rojo-Dom?nguez Arturo|Garc?a-Soto Jes?s|Pedraza-Reyes Mario (J Biomed Biotechnol;2009;201075, 2009)