Protein: Thermonuclease (Staphylococcus aureus)
UniProt: P00644
EC number: 3.1.31.1
Mutation: D177A
PDB: 1STN
Experiment dataset: ProTherm
Method: GdnHCl
Measure: Fluorescence
pH: 7
Buffer: Sodium phosphate 25 mM
Temperature: 20
Ion: NaCl 100 mM
dG: 2.1
ddG: 3.3
Cm: 0.28
m-value: 1.13
Reversibility: Unknown
Reference: Evidence for strained interactions between side-chains and the polypeptide backbone. — Shortle D|Stites W E|Meeker A K (J Mol Biol;235;27-32, 1994)