Protein: Subtilisin BPN' (Bacillus amyloliquefaciens)
UniProt: P00782
EC number: 3.4.21.62
Mutation: N325S
PDB: 1SUP|1SPB
Experiment dataset: ProTherm
Method: Thermal
Measure: DSC
pH: 8
Buffer: Tris-HCl 5.0 mM
Ion: KCl 50 mM
Tm: 62.5
dTm: 4
Reversibility: yes
Reference: Large increases in general stability for subtilisin BPN' through incremental changes in the free energy of unfolding. — Whitlow M|Pantoliano M W|Wood J F|Dodd S W|Hardman K D|Rollence M L|Bryan P N (Biochemistry;28;7205-13, 1989)