Protein: 1IGD ()
Mutation: F31D|F53D
Experiment dataset: MegaScale
Trypsin: -0.8418242140896661
Chymotrypsin: -2.5052315561280847
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)