Protein: Endolysin (Enterobacteria phage T4)
UniProt: P00720
EC number: 3.2.1.17
Mutation: A74P
PDB: 1L63|2LZM
Experiment dataset: ProTherm
Method: Thermal
Measure: CD
pH: 6.5
Buffer: Potassium phosphate 10 mM
Ion: KCl 150 mM
Tm: 50.8
dTm: -12.4
Reversibility: yes
Reference: Tolerance of T4 lysozyme to proline substitutions within the long interdomain alpha-helix illustrates the adaptability of proteins to potentially destabilizing lesions. — Matthews B W|Sauer U H|San D P (J Biol Chem;267;2393-9, 1992)