Protein: Ribonuclease (Bacillus amyloliquefaciens)
UniProt: P00648
EC number: 3.1.27.-
Mutation: I51A|Y125F
PDB: 1BNI
Experiment dataset: ProTherm
Method: Urea
Measure: Fluorescence
pH: 6.3
Buffer: MES 50 mM
Temperature: 25
Ion: NaCl 0.15 M
dG: 6.26
ddG: 2.53
Cm: 3.21
m-value: 1.95
Reversibility: yes
Reference: Rationally designing the accumulation of a folding intermediate of barnase by protein engineering. — Fersht A R|Sanz J M (Biochemistry;32;13584-92, 1993)