Protein: 5FWB ()
Mutation: T23D
Experiment dataset: MegaScale
dG: 3.146028867860008
Trypsin: 1.0732078665521174
Chymotrypsin: -0.1111322471888504
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)