Protein: Dihydrofolate reductase (Escherichia coli (strain K12))
UniProt: P0ABQ4
EC number: 1.5.1.3
Mutation: W74F
PDB: 5DFR
Experiment dataset: ProTherm
Method: Thermal
Measure: DSC
pH: 7.8
Buffer: Potassium Phosphate Buffer 10 mM
Tm: 50.5
dTm: -3.6
Reversibility: no
State: 2.0
Reference: Thermal stabilization of dihydrofolate reductase using monte carlo unfolding simulations and its functional consequences. — Tian Jian|Woodard Jaie C|Whitney Andrew (PLoS Comput Biol;11;e1004207, 2015)