Protein: Manganese-dependent inorganic pyrophosphatase (Bacillus subtilis (strain 168))
UniProt: P37487
EC number: 3.6.1.1
Mutation: D149E
PDB: 1K23
Experiment dataset: ProTherm
Method: Thermal
Measure: CD
pH: 7.2
Buffer: TES-KOH 21 mM
Ion: KCl 4 mM
Tm: 72
Reversibility: No
State: 3.0
Reference: Effects of active site mutations on the metal binding affinity, catalytic competence, and stability of the family II pyrophosphatase from Bacillus subtilis. — Halonen Pasi|Tammenkoski Marko|Niiranen Laila|Huopalahti Sauli|Parfenyev Alexey N|Goldman Adrian|Baykov Alexander|Lahti Reijo (Biochemistry;44;4004-10, 2005)