Protein: 5FWB ()
Mutation: V45D
Experiment dataset: MegaScale
dG: 2.958764004434138
Trypsin: 0.871331770807743
Chymotrypsin: -0.2026276874518225
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)