Protein: 2ROT ()
Mutation: E45M
Experiment dataset: MegaScale
dG: 2.754080549822862
ddG: 0.43704470488299
Trypsin: 0.6340306993351486
Chymotrypsin: -0.5025827663206198
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)