Protein: 1PWT ()
Mutation: D25T
Experiment dataset: MegaScale
dG: 3.198663736613061
ddG: -1.0034408383756022
Trypsin: 1.2430157074434152
Chymotrypsin: -0.1220732948685404
Stabilizing: False
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)