Protein: Guanyl-specific ribonuclease T1 (Aspergillus oryzae (strain ATCC 42149 / RIB 40))
UniProt: P00651
EC number: 4.6.1.24
Mutation: W85Y|H118A
PDB: 1YGW|1RN1|9RNT
Experiment dataset: ProTherm
Method: Thermal
Measure: DSC
pH: 6
Buffer: sodium acetate, calcium acetate 20 mM, 2 mM
Ion: Mops 50 mM
Tm: 53.2
dTm: -4
dH: 87
Reversibility: yes
Reference: X-ray crystallographic and calorimetric studies of the effects of the mutation Trp59-->Tyr in ribonuclease T1. — Schluckebier G|Backmann J|Granzin J|Schubert W D (Eur J Biochem;220;527-34, 1994)