Protein: Dihydrofolate reductase (Escherichia coli (strain K12))
UniProt: P0ABQ4
EC number: 1.5.1.3
Mutation: C85S|C152E
PDB: 5DFR
Experiment dataset: ProTherm
Method: Thermal
Measure: CD
pH: 7.8
Buffer: Potassium phosphate 10 mM
Tm: 33.4
dCp: 1.07
dHvH: 51
Reversibility: yes (100%)
State: 3.0
Reference: Detection of a stable intermediate in the thermal unfolding of a cysteine-free form of dihydrofolate reductase from Escherichia coli. — Iwakura M|Luo J|Matthews C R (Biochemistry;34;10669-75, 1995)