Protein: Ribonuclease HI (Escherichia coli (strain K12))
UniProt: P0A7Y4
EC number: 3.1.26.4
Mutation: Y28F|R29H|G30A|R31H
PDB: 2RN2
Experiment dataset: ProTherm
Method: Thermal
Measure: CD
pH: 5.5
Buffer: glycine-HCl 10 mM
Tm: 48.8
dTm: -3.2
dHvH: 80.5
Reversibility: yes
Reference: Stabilization of Escherichia coli ribonuclease HI by strategic replacement of amino acid residues with those from the thermophilic counterpart. — Oobatake M|Kanaya S|Nakamura H|Kimura S|Hashimoto T (J Biol Chem;267;21535-42, 1992)