Protein: Ribonuclease (Bacillus amyloliquefaciens)
UniProt: P00648
EC number: 3.1.27.-
Mutation: D59A|R157A
PDB: 1BNI
Experiment dataset: ProTherm
Method: Urea
Measure: Fluorescence
pH: 6.3
Buffer: MES-NaOH 450 mM
Temperature: 25
dG: 9.21
ddG: -0.39
Cm: 4.78
m-value: 1.93
Reversibility: yes
Reference: The folding of an enzyme. II. Substructure of barnase and the contribution of different interactions to protein stability. — Matouschek A|Serrano L|Fersht A R|Kellis J T|Cann P (J Mol Biol;224;783-804, 1992)