Protein: Dihydrofolate reductase (Escherichia coli (strain K12))
UniProt: P0ABQ4
EC number: 1.5.1.3
Mutation: R44L
PDB: 5DFR
Experiment dataset: ProTherm
Method: Urea
Measure: Fluorescence
pH: 7.8
Buffer: Potassium phosphate 10 mM
Temperature: 15
dG: 7.4
ddG: -1.5
Cm: 3.1
m-value: 2.4
Reversibility: yes (>95%)
Reference: Effect of single amino acid replacements on the folding and stability of dihydrofolate reductase from Escherichia coli. — Matthews C R|Onuffer J J|Perry K M|Touchette N A|Herndon C S|Gittelman M S (Biochemistry;26;2674-82, 1987)