Protein: Ribonuclease HI (Escherichia coli (strain K12))
UniProt: P0A7Y4
EC number: 3.1.26.4
Mutation: E135K
PDB: 2RN2
Experiment dataset: ProTherm
Method: Thermal
Measure: CD
pH: 5.5
Buffer: Sodium acetate 20 mM
Ion: NaCl 0.1 M
Tm: 51.9
dTm: -0.8
dHvH: 89.9
Reversibility: yes
Reference: A novel strategy for stabilization of Escherichia coli ribonuclease HI involving a screen for an intragenic suppressor of carboxyl-terminal deletions. — Akasako A|Haruki M|Oobatake M|Kanaya S|Noguchi E|Itaya M (J Biol Chem;269;26904-11, 1994)