Protein: Tryptophan synthase alpha chain (Escherichia coli (strain K12))
UniProt: P0A877
EC number: 4.2.1.20
Mutation: F22L
PDB: 1WQ5
Experiment dataset: ProTherm
Method: Urea
Measure: CD
pH: 7.8
Buffer: Potassium phosphate 10 mM
Temperature: 25
dG: 4.2
ddG: 0.8
Cm: 3.62
m-value: 1.2
Reversibility: yes (>90%)
State: 3.0
Reference: Effects of the phenylalanine-22----leucine, glutamic acid-49----methionine, glycine-234----aspartic acid, and glycine-234----lysine mutations on the folding and stability of the alpha subunit of tryptophan synthase from Escherichia coli. — Stackhouse T|Matthews C R|Beasty A M|Hurle M R|Manz J T|Onuffer J J (Biochemistry;25;2965-74, 1986)