Protein: 1W4F ()
Mutation: T25F|D35R
Experiment dataset: MegaScale
Trypsin: -1.400710545278729
Chymotrypsin: -2.22085650135326
Stabilizing: False
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)