Protein: 1EKL ()
Mutation: S4L|T54R
Experiment dataset: MegaScale
Trypsin: -1.6609082123156838
Chymotrypsin: -2.5488363746350533
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)