Protein: Ribonuclease (Bacillus amyloliquefaciens)
UniProt: P00648
EC number: 3.1.27.-
Mutation: I135V
PDB: 1BNI
Experiment dataset: ProTherm
Method: Urea
Measure: Fluorescence
pH: 6.3
Buffer: MES 450 mM
Temperature: 25
dG: 7.95
ddG: 1.47
Cm: 3.87
m-value: 2.06
Reversibility: yes (100%)
Reference: Energetics of complementary side-chain packing in a protein hydrophobic core. — Nyberg K|Fersht A R|Kellis J T (Biochemistry;28;4914-22, 1989)