Protein: Thermonuclease (Staphylococcus aureus)
UniProt: P00644
EC number: 3.1.31.1
Mutation: D225K
PDB: 1STN
Experiment dataset: ProTherm
Method: GdnHCl
Measure: Fluorescence
pH: 7
Buffer: sodium phosphate 25 mM
Temperature: 20
Ion: sodium chloride 100 mM
dG: 5.1
ddG: 0.3
Cm: 0.78
m-value: 6.47
Reversibility: Unknown
Reference: Changes in stability upon charge reversal and neutralization substitution in staphylococcal nuclease are dominated by favorable electrostatic effects. — Stites Wesley E|Schwehm Jeffery M|Fitch Carolyn A|Dang Bao N|Garc?a-Moreno E Bertrand (Biochemistry;42;1118-28, 2003)