Protein: 1EKL ()
Mutation: S4N|T54D
Experiment dataset: MegaScale
Trypsin: -1.4154594375967042
Chymotrypsin: -2.3489573266532826
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)