Protein: 2LYP ()
Mutation: E35D
Experiment dataset: MegaScale
dG: 2.493738412945186
Trypsin: 0.3031757523782906
Chymotrypsin: -0.5687624817776092
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)