Protein: 2KFV ()
Mutation: D20M
Experiment dataset: MegaScale
dG: 2.48035890625776
ddG: 0.17379117810732136
Trypsin: 0.6329727736150829
Chymotrypsin: -1.0091464217154564
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)