Protein: Guanyl-specific ribonuclease Sa (Kitasatospora aureofaciens)
UniProt: P05798
EC number: 4.6.1.24
Mutation: E74K
PDB: 1RGG
Experiment dataset: ProTherm
Method: Thermal
Measure: DSC
pH: 7
Buffer: PIPES 0.03 M
Temperature: 49
ddG: -0.9
Reversibility: yes (90-95%)
Reference: Contribution of active site residues to the activity and thermal stability of ribonuclease Sa. — Pace C Nick|Yakovlev Gennady I|Mitkevich Vladimir A|Shaw Kevin L|Trevino Saul|Newsom Stephanie|Makarov Alexander A (Protein Sci;12;2367-73, 2003)