Protein: Dihydrofolate reductase (Escherichia coli (strain K12))
UniProt: P0ABQ4
EC number: 1.5.1.3
Mutation: G121D
PDB: 5DFR
Experiment dataset: ProTherm
Method: Urea
Measure: CD
pH: 7
Buffer: Potassium phosphate 10 mM
Temperature: 15
dG: 6.27
ddG: -0.19
Cm: 2.7
m-value: 2.32
Reversibility: yes
Reference: Point mutations at glycine-121 of Escherichia coli dihydrofolate reductase: important roles of a flexible loop in the stability and function. — Gekko K|Kunori Y|Ichihara S|Kodama M|Takeuchi H (J Biochem;116;34-41, 1994)