Protein: Ribonuclease (Bacillus amyloliquefaciens)
UniProt: P00648
EC number: 3.1.27.-
Mutation: D101N
PDB: 1BNI
Experiment dataset: ProTherm
Method: Thermal
Measure: DSC
pH: 4.4
Buffer: Sodium acetate,Acetic acid, 6.08 mM,13.92 mM,
Tm: 44.57
dTm: -7.06
dH: 116.6
dHvH: 113.3
Reversibility: yes (>95%)
Reference: Extrapolation to water of kinetic and equilibrium data for the unfolding of barnase in urea solutions. — Matouschek A|Johnson C M|Fersht A R|Matthews J M (Protein Eng;7;1089-95, 1994)