Protein: Ribonuclease (Bacillus amyloliquefaciens)
UniProt: P00648
EC number: 3.1.27.-
Mutation: Y60A|Y64A
PDB: 1BNI
Experiment dataset: ProTherm
Method: Urea
Measure: Fluorescence
pH: 6.3
Buffer: MES 1.00 M
Temperature: 25
Ion: NaCl 613 mM
dG: 5.39
ddG: 4.76
Cm: 2.43
m-value: 2.22
Reversibility: yes
Reference: Aromatic-aromatic interactions and protein stability. Investigation by double-mutant cycles. — Serrano L|Bycroft M|Fersht A R (J Mol Biol;218;465-75, 1991)