Protein: Ribonuclease HI (Escherichia coli (strain K12))
UniProt: P0A7Y4
EC number: 3.1.26.4
Mutation: H127A
PDB: 2RN2
Experiment dataset: ProTherm
Method: GdnHCl
Measure: CD
pH: 8.2
Buffer: Tris-HCl 20 mM
Temperature: 25
dG: 8.18
ddG: 0.31
Cm: 1.59
Reversibility: yes
Reference: pH-dependent thermostabilization of Escherichia coli ribonuclease HI by histidine to alanine substitutions. — Oobatake M|Kanaya S|Nakamura H|Ikehara M (J Biotechnol;28;117-36, 1993)