Protein: Ribonuclease (Bacillus amyloliquefaciens)
UniProt: P00648
EC number: 3.1.27.-
Mutation: D59A|T63R
PDB: 1BNI
Experiment dataset: ProTherm
Method: Urea
Measure: Fluorescence
pH: 6.3
Buffer: MES-NaOH 50 mM
Temperature: 25
dG: 9.88
ddG: 0.13
Cm: 4.4
m-value: 2.24
Reversibility: yes
Reference: Estimating the contribution of engineered surface electrostatic interactions to protein stability by using double-mutant cycles. — Serrano L|Bycroft M|Horovitz A|Avron B|Fersht A R (Biochemistry;29;9343-52, 1990)