Protein: Endolysin (Enterobacteria phage T4)
UniProt: P00720
EC number: 3.2.1.17
Mutation: L99A|E108V
PDB: 1L63|2LZM
Experiment dataset: ProTherm
Method: Thermal
Measure: CD
pH: 5.4
Buffer: Sodium acetate 10 mM
Temperature: 65.2
Ion: NaCl 0.10 M
ddG: 3.1
Reversibility: yes(>80%)
Reference: Structural analysis of a non-contiguous second-site revertant in T4 lysozyme shows that increasing the rigidity of a protein can enhance its stability. — Baase W A|Lindstrom J D|Poteete A R|Weaver L H|Wray J W (J Mol Biol;292;1111-20, 1999)