Protein: Ribonuclease HI (Escherichia coli (strain K12))
UniProt: P0A7Y4
EC number: 3.1.26.4
Mutation: K91R|D94E|K95G
PDB: 2RN2
Experiment dataset: ProTherm
Method: GdnHCl
Measure: CD
pH: 5.5
Buffer: glycine-HCl 10 mM
Temperature: 25
dG: 9.14
ddG: 0.01
Cm: 2.1
m-value: 4.36
Reversibility: yes
Reference: Stabilization of Escherichia coli ribonuclease HI by strategic replacement of amino acid residues with those from the thermophilic counterpart. — Oobatake M|Kanaya S|Nakamura H|Kimura S|Hashimoto T (J Biol Chem;267;21535-42, 1992)