Protein: Guanyl-specific ribonuclease Sa (Kitasatospora aureofaciens)
UniProt: P05798
EC number: 4.6.1.24
Mutation: R40E|R63E|R65A|R68A|R69A
PDB: 1RGG
Experiment dataset: ProTherm
Method: Urea
Measure: CD
pH: 7
Buffer: MOPS 30 mM
Temperature: 25
dG: 3.9
ddG: 2.5
Cm: 4.6
m-value: 0.85
Reversibility: Unknown
Reference: Urea denatured state ensembles contain extensive secondary structure that is increased in hydrophobic proteins. — Nick Pace C|Takano Kazufumi|Scholtz J Martin|Huyghues-Despointes Beatrice M P|Fu Hailong|Grimsley Gerald R (Protein Sci;19;929-43, 2010)