Protein: 5ECA ()
Mutation: S58D
Experiment dataset: MegaScale
dG: 3.24599833807995
ddG: 0.32494835139188716
Trypsin: 1.1223528675291798
Chymotrypsin: 0.0906797924678859
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)