Protein: Thermonuclease (Staphylococcus aureus)
UniProt: P00644
EC number: 3.1.31.1
Mutation: D225A
PDB: 1STN
Experiment dataset: ProTherm
Method: GdnHCl
Measure: Fluorescence
pH: 7
Buffer: Sodium phosphate 25 mM
Temperature: 20
Ion: NaCl 100 mM
dG: 5.5
ddG: 0.1
Cm: 0.79
m-value: 1.01
Reversibility: yes
Reference: Contributions of the ionizable amino acids to the stability of staphylococcal nuclease. — Shortle D|Garcia-Moreno B|Meeker A K (Biochemistry;35;6443-9, 1996)