Protein: 1EKL ()
Mutation: S4E|T54D
Experiment dataset: MegaScale
Trypsin: -1.4669612051134644
Chymotrypsin: -2.379648223133521
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)