Protein: Ribonuclease HI (Escherichia coli (strain K12))
UniProt: P0A7Y4
EC number: 3.1.26.4
Mutation: V74A
PDB: 2RN2
Experiment dataset: ProTherm
Method: Thermal
Measure: CD
pH: 5.5
Buffer: sodium acetate 20 mM
Ion: NaCl 0.1 M
dTm: -12.7
ddG: 3.4
Reversibility: yes
Reference: Stabilization of Escherichia coli ribonuclease HI by cavity-filling mutations within a hydrophobic core. — Kanaya S|Nakamura H|Ishikawa K|Morikawa K (Biochemistry;32;6171-8, 1993)