Protein: Ribonuclease (Bacillus amyloliquefaciens)
UniProt: P00648
EC number: 3.1.27.-
Mutation: H65G|W141L
PDB: 1BNI
Experiment dataset: ProTherm
Method: Urea
Measure: Fluorescence
pH: 9
Buffer: MES,Tris, 150 mM,150 mM,
Temperature: 25
Ion: NaCl 1 M
dG: 9.72
ddG: -0.31
Cm: 4.96
m-value: 1.8
Reversibility: Unknown
Reference: Histidine-aromatic interactions in barnase. Elevation of histidine pKa and contribution to protein stability. — Sancho J|Loewenthal R|Fersht A R (J Mol Biol;224;759-70, 1992)