Protein: Endolysin (Enterobacteria phage T4)
UniProt: P00720
EC number: 3.2.1.17
Mutation: H31N|D70N
PDB: 1L63|2LZM
Experiment dataset: ProTherm
Method: Thermal
Measure: CD
pH: 5.5
Buffer: Potassium phosphate 10 mM
Ion: KCl 0.15 M
Tm: 55
dTm: -11
Reversibility: yes
Reference: pH-induced denaturation of proteins: a single salt bridge contributes 3-5 kcal/mol to the free energy of folding of T4 lysozyme. — Anderson D E|Becktel W J|Dahlquist F W (Biochemistry;29;2403-8, 1990)