Protein: Ribonuclease (Bacillus amyloliquefaciens)
UniProt: P00648
EC number: 3.1.27.-
Mutation: K66R
PDB: 1BNI
Experiment dataset: ProTherm
Method: Urea
Measure: Fluorescence
pH: 6.3
Buffer: MES 50 mM
Temperature: 25
dG: 9.7
ddG: -0.88
Cm: 4.6
m-value: 2.08
Reversibility: yes
Reference: Step-wise mutation of barnase to binase. A procedure for engineering increased stability of proteins and an experimental analysis of the evolution of protein stability. — Serrano L|Fersht A R|Day A G (J Mol Biol;233;305-12, 1993)