Protein: Endolysin (Enterobacteria phage T4)
UniProt: P00720
EC number: 3.2.1.17
Mutation: K135E|K147E
PDB: 1L63|2LZM
Experiment dataset: ProTherm
Method: Thermal
Measure: CD
pH: 5.3
Buffer: Potassium phosphate 20 mM
Ion: KCl 25 mM
Tm: 63
dTm: -3.7
Reversibility: yes
Reference: Cumulative site-directed charge-change replacements in bacteriophage T4 lysozyme suggest that long-range electrostatic interactions contribute little to protein stability. — Baase W A|Dao-pin S|S?derlind E|Wozniak J A|Sauer U (J Mol Biol;221;873-87, 1991)