Protein: Ribonuclease HI (Escherichia coli (strain K12))
UniProt: P0A7Y4
EC number: 3.1.26.4
Mutation: V74L
PDB: 2RN2
Experiment dataset: ProTherm
Method: Thermal
Measure: CD
pH: 5.5
Buffer: Sodium acetate 10 mM
Ion: NaCl 0.1 M
Tm: 55.8
dTm: 3.3
dHvH: 91.2
Reversibility: yes
Reference: High resistance of Escherichia coli ribonuclease HI variant with quintuple thermostabilizing mutations to thermal denaturation, acid denaturation, and proteolytic degradation. — Akasako A|Haruki M|Oobatake M|Kanaya S (Biochemistry;34;8115-22, 1995)