Protein: Subtilisin BPN' (Bacillus amyloliquefaciens)
UniProt: P00782
EC number: 3.4.21.62
Mutation: T129C
PDB: 1SUP|1SPB
Experiment dataset: ProTherm
Method: Thermal
Measure: DSC
pH: 8
Buffer: Tris-HCl 50 mM
Ion: KCl 50 mM
Tm: 56.4
dTm: -2.5
Reversibility: Unknown
Reference: Protein engineering of subtilisin BPN': enhanced stabilization through the introduction of two cysteines to form a disulfide bond. — Pantoliano M W|Wood J F|Rollence M L|Bryan P N|Poulos T L|Ladner R C (Biochemistry;26;2077-82, 1987)