Protein: Immunoglobulin G-binding protein G (Streptococcus sp. group G)
UniProt: P06654
Mutation: K254D
PDB: 1EM7|1PGA
Experiment dataset: ProTherm
Method: GdnHCl
Measure: fluorescence
pH: 6.5
Buffer: NaPO4 buffer 
Temperature: 25
dG: 4.08
ddG: 0.29
Cm: 2.16
m-value: -1.89
Reversibility: yes
State: 2.0
Reference: Protein stability engineering insights revealed by domain-wide comprehensive mutagenesis. — Mayo Stephen L|Nisthal Alex|Wang Connie Y|Ary Marie L (Proc Natl Acad Sci U S A;116;16367-16377, 2019)