Protein: Ribonuclease HI (Escherichia coli (strain K12))
UniProt: P0A7Y4
EC number: 3.1.26.4
Mutation: A52F|V74A
PDB: 2RN2
Experiment dataset: ProTherm
Method: Thermal
Measure: CD
pH: 3.2
Buffer: glycine-HCl 10 mM
Tm: 48.4
dTm: -4.6
dHvH: 85.8
Reversibility: yes
Reference: Conformational stabilities of Escherichia coli RNase HI variants with a series of amino acid substitutions at a cavity within the hydrophobic core. — Akasako A|Haruki M|Oobatake M|Kanaya S (J Biol Chem;272;18686-93, 1997)