Protein: 2K5P ()
Mutation: T21F|D50A
Experiment dataset: MegaScale
dG: 2.862678453105163
ddG: 0.338356241806423
Trypsin: 1.1394902008603038
Chymotrypsin: -0.1883322456045123
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)