Protein: Thermonuclease (Staphylococcus aureus)
UniProt: P00644
EC number: 3.1.31.1
Mutation: V148I|I174L
PDB: 1STN
Experiment dataset: ProTherm
Method: GdnHCl
Measure: Fluorescence
pH: 7
Buffer: Sodium phosphate 25 mM
Temperature: 20
Ion: NaCl 100 mM
dG: 3.5
ddG: 1.9
Cm: 0.59
m-value: 0.88
Reversibility: yes
Reference: Energetics of side chain packing in staphylococcal nuclease assessed by systematic double mutant cycles. — Chen J|Stites W E (Biochemistry;40;14004-11, 2001)