Protein: Ribonuclease (Bacillus amyloliquefaciens)
UniProt: P00648
EC number: 3.1.27.-
Mutation: D59A|R157A
PDB: 1BNI
Experiment dataset: ProTherm
Method: Urea
Measure: Fluorescence
pH: 6.3
Buffer: MES 50 mM
Temperature: 25
dG: 10.38
ddG: -0.45
Cm: 4.78
m-value: 2.14
Reversibility: yes
Reference: Strength and co-operativity of contributions of surface salt bridges to protein stability. — Serrano L|Bycroft M|Horovitz A|Avron B|Fersht A R (J Mol Biol;216;1031-44, 1990)