Protein: Large ribosomal subunit protein bL9 (Geobacillus stearothermophilus)
UniProt: P02417
Mutation: D23N
PDB: 1DIV
Experiment dataset: ProTherm
Method: Thermal
Measure: CD
pH: 5.4
Buffer: sodium phosphate 10 mM
Ion: sodium chloride 100 mM
Tm: 67
dTm: -10
Reversibility: yes(>90%)
Reference: Surface salt bridges, double-mutant cycles, and protein stability: an experimental and computational analysis of the interaction of the Asp 23 side chain with the N-terminus of the N-terminal domain of the ribosomal protein l9. — Raleigh Daniel P|Luisi Donna L|Snow Christopher D|Lin Jo-Jin|Hendsch Zachary S|Tidor Bruce (Biochemistry;42;7050-60, 2003)