Protein: Endolysin (Enterobacteria phage T4)
UniProt: P00720
EC number: 3.2.1.17
Mutation: T59D
PDB: 1L63|2LZM
Experiment dataset: ProTherm
Method: Thermal
Measure: CD
pH: 6.5
Buffer: Potassium phosphate 10 mM
Ion: KCl 15 mM
Tm: 59.9
dTm: -3.1
Reversibility: yes
Reference: Dissection of helix capping in T4 lysozyme by structural and thermodynamic analysis of six amino acid substitutions at Thr 59. — Baase W A|Matthews B W|Sauer U|Becktel W J|Bell J A (Biochemistry;31;3590-6, 1992)