Protein: Endolysin (Enterobacteria phage T4)
UniProt: P00720
EC number: 3.2.1.17
Mutation: N144E|K147M
PDB: 1L63|2LZM
Experiment dataset: ProTherm
Method: Thermal
Measure: CD
pH: 5.5
Buffer: HCl 10 mM
Ion: KCl 0.15 M
Tm: 66.6
dTm: 1.2
dHvH: 139
Reversibility: yes
Reference: Contributions of engineered surface salt bridges to the stability of T4 lysozyme determined by directed mutagenesis. — Nicholson H|Matthews B W|Sauer U|Sun D P (Biochemistry;30;7142-53, 1991)