Protein: Endolysin (Enterobacteria phage T4)
UniProt: P00720
EC number: 3.2.1.17
Mutation: L133D
PDB: 1L63|2LZM
Experiment dataset: ProTherm
Method: Thermal
Measure: CD
pH: 10.4
Buffer: Potassium phosphate 20 mM
Ion: KCl 25 mM
Tm: 28.9
dTm: -19
dCp: 1.8
dHvH: 47.1
Reversibility: yes(20%)
Reference: Structural and thermodynamic consequences of burying a charged residue within the hydrophobic core of T4 lysozyme. — Baase W A|Matthews B W|Dao-pin S|Anderson D E|Dahlquist F W (Biochemistry;30;11521-9, 1991)