Protein: 2K9D ()
Mutation: D32A
Experiment dataset: MegaScale
dG: 4.172110424973294
ddG: 0.4294260976863859
Trypsin: 1.5019514006099897
Chymotrypsin: 1.00056678287324
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)