Protein: 2K5P ()
Mutation: T21F|D50W
Experiment dataset: MegaScale
dG: 2.71424836243499
ddG: 0.18992615113625
Trypsin: 1.006534164921933
Chymotrypsin: -0.3121663533814653
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)