Protein: 1W4F ()
Mutation: T25F|D35Y
Experiment dataset: MegaScale
dG: 0.2129392329545102
ddG: -3.5714651970743505
Trypsin: -0.694131289836762
Stabilizing: False
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)