Protein: 2LSS ()
Mutation: D53F|N65Q
Experiment dataset: MegaScale
dG: 3.8394102067005
Trypsin: 1.2838112502301715
Chymotrypsin: 0.2650941918404683
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)