Protein: Thioredoxin 1 (Escherichia coli (strain K12))
UniProt: P0AA25
Mutation: D27A
PDB: 2TRX|2H6X
Experiment dataset: ProTherm
Method: GdnHCl
Measure: CD
pH: 7
Buffer: TES 50 mM
Temperature: 25
dG: 13.2
ddG: -3.7
m-value: 3.2
Reversibility: Unknown
Reference: The conserved, buried aspartic acid in oxidized Escherichia coli thioredoxin has a pKa of 7.5. Its titration produces a related shift in global stability. — Langsetmo K|Woodward C|Fuchs J A (Biochemistry;30;7603-9, 1991)