Protein: Ribonuclease (Bacillus amyloliquefaciens)
UniProt: P00648
EC number: 3.1.27.-
Mutation: S132C|H149C
PDB: 1BNI
Experiment dataset: ProTherm
Method: GdnHCl
Measure: Fluorescence
pH: 6.3
Buffer: MES 450 mM
Temperature: 25
dG: 12.9
ddG: -4.1
Cm: 3
m-value: 4.3
Reversibility: yes
Reference: Engineered disulfide bonds as probes of the folding pathway of barnase: increasing the stability of proteins against the rate of denaturation. — Clarke J|Fersht A R (Biochemistry;32;4322-9, 1993)