Protein: Alpha-amylase (Pseudoalteromonas haloplanktis)
UniProt: P29957
EC number: 3.2.1.1
Mutation: N174D|V220F
PDB: 1AQH
Experiment dataset: ProTherm
Method: Thermal
Measure: DSC
pH: 7.2
Buffer: MOPS 30 mM
Ion: NaCl 50 mM
Tm: 46.4
dH: 272
dHvH: 271
Reversibility: yes(91 %)
Reference: Structural determinants of cold adaptation and stability in a large protein. — D'Amico S|Gerday C|Feller G (J Biol Chem;276;25791-6, 2001)