Protein: Thermonuclease (Staphylococcus aureus)
UniProt: P00644
EC number: 3.1.31.1
Mutation: T115S|A172S
PDB: 1STN
Experiment dataset: ProTherm
Method: GdnHCl
Measure: Fluorescence
pH: 7
Buffer: Sodium phosphate 25 mM
Temperature: 20
Ion: NaCl 100 mM
ddG: 3.16
m-value: 1.2
Reversibility: yes
Reference: Patterns of nonadditivity between pairs of stability mutations in staphylococcal nuclease. — Shortle D|Green S M (Biochemistry;32;10131-9, 1993)