Protein: Ribonuclease (Bacillus amyloliquefaciens)
UniProt: P00648
EC number: 3.1.27.-
Mutation: T73H|D101N
PDB: 1BNI
Experiment dataset: ProTherm
Method: Urea
Measure: NMR
pH: 8.9
Buffer: Tris 30 mM
Temperature: 25
Ion: NaCl 1 M
dG: 3.78
ddG: 4.27
Reversibility: Unknown
Reference: Histidine residues at the N- and C-termini of alpha-helices: perturbed pKas and protein stability. — Serrano L|Sancho J|Fersht A R (Biochemistry;31;2253-8, 1992)