Protein: 2K5N ()
Mutation: A35E
Experiment dataset: MegaScale
dG: 4.228274761152509
ddG: 0.21544887064264273
Trypsin: 1.8918485868284511
Chymotrypsin: 0.4988052275822177
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)