Protein: Dihydrofolate reductase (Escherichia coli (strain K12))
UniProt: P0ABQ4
EC number: 1.5.1.3
Mutation: C85S|C152E
PDB: 5DFR
Experiment dataset: ProTherm
Method: Urea
Measure: CD
pH: 7.8
Buffer: Potassium phosphate 10 mM
Temperature: 15
dG: 4.4
m-value: 2.5
Reversibility: yes (>95%)
Reference: Collapse of parallel folding channels in dihydrofolate reductase from Escherichia coli by site-directed mutagenesis. — Iwakura M|Matthews C R|Jones B E|Falzone C J (Biochemistry;32;13566-74, 1993)