Protein: 2K9D ()
Mutation: D25N
Experiment dataset: MegaScale
dG: 3.791726764155869
ddG: 0.04904243686896104
Trypsin: 1.2580274217747445
Chymotrypsin: 0.6996538885195922
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)