Protein: Repressor protein cI (Escherichia phage lambda)
UniProt: P03034
Mutation: Y89C|G47A|G49A
PDB: 3BDN
Experiment dataset: ProTherm
Method: Thermal
Measure: DSC
pH: 8
Buffer: Potassium phosphate 10 mM
Temperature: 60
Ion: KCl 200 mM
ddG: -4.2
Reversibility: yes (95%)
Reference: Combining thermostable mutations increases the stability of lambda repressor. — Freire E|Stearman R S|Pabo C O|Frankel A D|Liu B S (Biochemistry;27;7571-4, 1988)