Protein: 1S1N ()
Mutation: E25D
Experiment dataset: MegaScale
dG: 4.39087334669388
ddG: 1.2535502555526
Trypsin: 1.753994894432013
Chymotrypsin: 0.999050108483653
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)