Protein: 5FWB ()
Mutation: L33A
Experiment dataset: MegaScale
dG: 2.405284483795508
Trypsin: 0.4439345270718897
Chymotrypsin: -0.6212095712807273
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)