Protein: Large ribosomal subunit protein bL9 (Geobacillus stearothermophilus)
UniProt: P02417
Mutation: D23A
PDB: 1DIV
Experiment dataset: ProTherm
Method: Urea
Measure: CD
pH: 5.4
Buffer: sodium phosphate 10 mM
Temperature: 25
Ion: sodium chloride 100 mM
dG: 3.27
ddG: -1.25
Cm: 4.36
m-value: 0.75
Reversibility: yes(>90%)
Reference: Surface salt bridges, double-mutant cycles, and protein stability: an experimental and computational analysis of the interaction of the Asp 23 side chain with the N-terminus of the N-terminal domain of the ribosomal protein l9. — Raleigh Daniel P|Luisi Donna L|Snow Christopher D|Lin Jo-Jin|Hendsch Zachary S|Tidor Bruce (Biochemistry;42;7050-60, 2003)