Protein: Ribonuclease HI (Escherichia coli (strain K12))
UniProt: P0A7Y4
EC number: 3.1.26.4
Mutation: H114A
PDB: 2RN2
Experiment dataset: ProTherm
Method: GdnHCl
Measure: Fluorescence
pH: 5.5
Buffer: Sodium acetate 20 mM
Temperature: 25
Ion: NaCl 0.1 M
dG: 6.63
ddG: 2.63
Cm: 1.57
m-value: 4.23
Reversibility: yes
Reference: Effect of mutagenesis at each of five histidine residues on enzymatic activity and stability of ribonuclease H from Escherichia coli. — Kanaya S|Katayanagi K|Morikawa K|Inoue H|Ohtsuka E (Eur J Biochem;198;437-40, 1991)