Protein: Colicin-E9 immunity protein (Escherichia coli)
UniProt: P13479
Mutation: A25G
PDB: 1IMQ
Experiment dataset: ProTherm
Method: Urea
Measure: Fluorescence
pH: 6
Buffer: sodium phosphate 50 mM
Temperature: 10
Ion: Na2SO4 0.4 M
dG: 5.76
ddG: 0.14
Reversibility: Unknown
Reference: Switching two-state to three-state kinetics in the helical protein Im9 via the optimisation of stabilising non-native interactions by design. — Beddard Godfrey S|Radford Sheena E|Friel Claire T (J Mol Biol;342;261-73, 2004)