Protein: Ribonuclease (Bacillus amyloliquefaciens)
UniProt: P00648
EC number: 3.1.27.-
Mutation: D101A
PDB: 1BNI
Experiment dataset: ProTherm
Method: Thermal
Measure: Fluorescence
pH: 6.3
Buffer: MES 50 mM
Temperature: 25
dG: 6.9
ddG: 3.3
Reversibility: Unknown
Reference: pKA values of carboxyl groups in the native and denatured states of barnase: the pKA values of the denatured state are on average 0.4 units lower than those of model compounds. — Oliveberg M|Fersht A R|Arcus V L (Biochemistry;34;9424-33, 1995)