Protein: Carboxymethylenebutenolidase (Pseudomonas knackmussii (strain DSM 6978 / CCUG 54928 / LMG 23759 / B13))
UniProt: P0A115
EC number: 3.1.1.45
Mutation: Q110L|K154D
PDB: 4U2B
Experiment dataset: ProTherm
Method: Thermal
Measure: fluorescence
pH: 7.5
Buffer: Hepes 50 mM
Tm: 54.8
dTm: 3.3
Reversibility: Unknown
Reference: Compensatory stabilizing role of surface mutations during the directed evolution of dienelactone hydrolase for enhanced activity. — Porter Joanne L|Collyer Charles A|Ollis David L (Protein J;34;82-9, 2015)