Protein: 1EKL ()
Mutation: S4I|T54Q
Experiment dataset: MegaScale
Trypsin: -1.2794864244363389
Chymotrypsin: -2.37253944731734
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)