Protein: Phosphocarrier protein HPr (Escherichia coli (strain K12))
UniProt: P0AA04
Mutation: S31A
PDB: 1CM2|1POH
Experiment dataset: ProTherm
Method: Urea
Measure: CD
pH: 7
Buffer: Potassium phosphate 50 mM
Temperature: 30
dG: 4.61
ddG: 0.33
Reversibility: yes
Reference: Investigation of a side-chain-side-chain hydrogen bond by mutagenesis, thermodynamics, and NMR spectroscopy. — Waygood E B|Scholtz J M|Klevit R E|Hammen P K|Anderson J W (Protein Sci;4;936-44, 1995)