Protein: Thermonuclease (Staphylococcus aureus)
UniProt: P00644
EC number: 3.1.31.1
Mutation: P199L|N200D
PDB: 1STN
Experiment dataset: ProTherm
Method: GdnHCl
Measure: Fluorescence
pH: 7
Buffer: Sodium phosphate 25 mM
Temperature: 20
Ion: NaCl 100 mM
ddG: 1
m-value: 0.86
Reversibility: yes
Reference: Patterns of nonadditivity between pairs of stability mutations in staphylococcal nuclease. — Shortle D|Green S M (Biochemistry;32;10131-9, 1993)