Protein: 2K5N ()
Mutation: N53Y|S58A
Experiment dataset: MegaScale
dG: 4.295267406902818
ddG: 0.2824415163929519
Trypsin: 1.6345241662130263
Chymotrypsin: 0.4675339115764453
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)