Protein: Dihydrofolate reductase (Escherichia coli (strain K12))
UniProt: P0ABQ4
EC number: 1.5.1.3
Mutation: V88A
PDB: 5DFR
Experiment dataset: ProTherm
Method: Urea
Measure: CD
pH: 7.2
Buffer: Potassium phosphate 20 mM
Temperature: 20
dG: 5.55
ddG: -0.39
Cm: 3.4
m-value: 1.62
Reversibility: Unknown
Reference: Effects of point mutations in a hinge region on the stability, folding, and enzymatic activity of Escherichia coli dihydrofolate reductase. — Frieden C|Ahrweiler P M (Biochemistry;30;7801-9, 1991)