Protein: 1IGD ()
Mutation: F31D|F53Q
Experiment dataset: MegaScale
Trypsin: -0.7982396532207723
Chymotrypsin: -2.5912686697370573
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)