Protein: 2K5H ()
Mutation: S3I
Experiment dataset: MegaScale
dG: 4.69653189662518
ddG: -0.04423719094826417
Chymotrypsin: 1.3827711850186426
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)