Protein: Ribonuclease HI (Escherichia coli (strain K12))
UniProt: P0A7Y4
EC number: 3.1.26.4
Mutation: E48D
PDB: 2RN2
Experiment dataset: ProTherm
Method: Thermal
Measure: CD
pH: 9
Buffer: glycine-NaOH 50 mM
Tm: 46.2
dTm: -0.8
Reversibility: yes
Reference: Thermal stability of Escherichia coli ribonuclease HI and its active site mutants in the presence and absence of the Mg2+ ion. Proposal of a novel catalytic role for Glu48. — Oobatake M|Kanaya S|Liu Y (J Biol Chem;271;32729-36, 1996)