Protein: 1W4F ()
Mutation: I3D
Experiment dataset: MegaScale
dG: 3.593364270189016
ddG: -0.191040159839845
Trypsin: 1.184039504140893
Chymotrypsin: 0.706308801198033
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)