Protein: Guanyl-specific ribonuclease T1 (Aspergillus oryzae (strain ATCC 42149 / RIB 40))
UniProt: P00651
EC number: 4.6.1.24
Mutation: N70S
PDB: 1YGW|1RN1|9RNT
Experiment dataset: ProTherm
Method: Thermal
Measure: CD
pH: 7
Buffer: MOPS 30 mM
Tm: 45.8
dTm: -5
dHvH: 89.6
Reversibility: yes (>95%)
Reference: Contribution of a conserved asparagine to the conformational stability of ribonucleases Sa, Ba, and T1. — Giletto A|Sevcik J|Urbanikova L|Dauter Z|Pace C N|Hebert E J|Wilson K S (Biochemistry;37;16192-200, 1998)