Protein: Ribonuclease (Bacillus amyloliquefaciens)
UniProt: P00648
EC number: 3.1.27.-
Mutation: I135A
PDB: 1BNI
Experiment dataset: ProTherm
Method: Thermal
Measure: Fluorescence
pH: 6.3
Buffer: MES 50 mM
Temperature: 48
ddG: 3.9
Reversibility: yes (>85%)
Reference: Energetics of complementary side-chain packing in a protein hydrophobic core. — Nyberg K|Fersht A R|Kellis J T (Biochemistry;28;4914-22, 1989)