Protein: 3-isopropylmalate dehydrogenase (Bacillus subtilis (strain 168))
UniProt: P05645
EC number: 1.1.1.85
Mutation: M256F
Experiment dataset: ProTherm
Method: Thermal
Measure: CD
pH: 7.6
Buffer: potassium phosphate 20 mM
dTm: -12.5
Reversibility: No
Reference: Increased thermal stability against irreversible inactivation of 3-isopropylmalate dehydrogenase induced by decreased van der Waals volume at the subunit interface. — Yamagishi Akihiko|Ohkuri Takatoshi (Protein Eng;16;615-21, 2003)