Protein: Ribonuclease (Bacillus amyloliquefaciens)
UniProt: P00648
EC number: 3.1.27.-
Mutation: I135V
PDB: 1BNI
Experiment dataset: ProTherm
Method: Thermal
Measure: DSC
pH: 4.4
Buffer: Sodium acetate,Acetic acid, 6.08 mM,13.92 mM,
Tm: 48.11
dTm: -3.37
dH: 119.2
dHvH: 124.8
Reversibility: yes (>95%)
Reference: Extrapolation to water of kinetic and equilibrium data for the unfolding of barnase in urea solutions. — Matouschek A|Johnson C M|Fersht A R|Matthews J M (Protein Eng;7;1089-95, 1994)