Protein: Thermonuclease (Staphylococcus aureus)
UniProt: P00644
EC number: 3.1.31.1
Mutation: V196T
PDB: 1STN
Experiment dataset: ProTherm
Method: GdnHCl
Measure: Fluorescence
pH: 7
Buffer: Sodium phosphate 25 mM
Temperature: 20
Ion: NaCl 100 mM
dG: 5.3
ddG: 0.3
Cm: 0.78
m-value: 1.02
Reversibility: yes
Reference: Energetic contribution of side chain hydrogen bonding to the stability of staphylococcal nuclease. — Stites W E|Byrne M P|Manuel R L|Lowe L G (Biochemistry;34;13949-60, 1995)