Protein: Chitosanase (Streptomyces sp. (strain N174))
UniProt: P33665
EC number: 3.2.1.132
Mutation: W68F|W141F
PDB: 1CHK
Experiment dataset: ProTherm
Method: Thermal
Measure: Fluorescence
pH: 7
Buffer: sodium phosphate 50 mM
Temperature: 43.2
ddG: 8.03
Reversibility: yes(80%)
Reference: Thermal unfolding of chitosanase from Streptomyces sp. N174: role of tryptophan residues in the protein structure stabilization. — Honda Y|Fukamizo T|Okajima T|Goto S|Boucher I|Brzezinski R (Biochim Biophys Acta;1429;365-76, 1999)