Protein: 1PWT ()
Mutation: D25Q
Experiment dataset: MegaScale
dG: 4.603090083225882
ddG: 0.4009855082372189
Trypsin: 1.9885289298101765
Chymotrypsin: 0.9618160757853196
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)