Protein: 1PWT ()
Mutation: D25W
Experiment dataset: MegaScale
dG: 4.20459563546427
ddG: 0.0024910604756067656
Trypsin: 1.8125706663853736
Chymotrypsin: 0.568979960139242
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)