Protein: 1PWT ()
Mutation: D44N
Experiment dataset: MegaScale
dG: 4.172594806183223
ddG: -0.02950976880543976
Trypsin: 1.8998805195811408
Chymotrypsin: 0.7186569634273973
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)