Protein: Ribonuclease HI (Escherichia coli (strain K12))
UniProt: P0A7Y4
EC number: 3.1.26.4
Mutation: K91R|D94E
PDB: 2RN2
Experiment dataset: ProTherm
Method: Thermal
Measure: CD
pH: 5.5
Buffer: glycine-HCl 10 mM
Tm: 50.2
dTm: -1.8
dHvH: 87.5
Reversibility: yes
Reference: Thermostabilization of Escherichia coli ribonuclease HI by replacing left-handed helical Lys95 with Gly or Asn. — Kanaya S|Nakamura H|Kimura S (J Biol Chem;267;22014-7, 1992)