Protein: 1PWT ()
Mutation: D25F
Experiment dataset: MegaScale
dG: 2.211553407225796
ddG: -1.9905511677628671
Trypsin: 0.4554973783593172
Chymotrypsin: -1.0275839212901443
Stabilizing: False
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)