Protein: 2K5H ()
Mutation: A50N
Experiment dataset: MegaScale
dG: 4.485000017838516
ddG: -0.25576906973492797
Trypsin: 2.201073485823668
Chymotrypsin: 1.3060729357467502
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)