Protein: Phosphoenolpyruvate-protein phosphotransferase (Escherichia coli (strain K12))
UniProt: P08839
EC number: 2.7.3.9
Mutation: H189E
PDB: 1ZYM|1EZA
Experiment dataset: ProTherm
Method: Thermal
Measure: CD
pH: 7.5
Buffer: Potassium phosphate 10 mM
Tm: 52.1
dHvH: 128
Reversibility: yes
Reference: Conformational stability changes of the amino terminal domain of enzyme I of the Escherichia coli phosphoenolpyruvate: sugar phosphotransferase system produced by substituting alanine or glutamate for the active-site histidine 189: implications for phosphorylation effects. — Ginsburg A|Szczepanowski R H|Ruvinov S B|Nosworthy N J (Protein Sci;9;1085-94, 2000)