Protein: Dihydrofolate reductase (Escherichia coli (strain K12))
UniProt: P0ABQ4
EC number: 1.5.1.3
Mutation: E139K
PDB: 5DFR
Experiment dataset: ProTherm
Method: Urea
Measure: Absorbance
pH: 7.8
Buffer: Potassium phosphate 10 mM
Temperature: 15
dG: 4.94
ddG: 0.98
Cm: 2.11
m-value: 2.34
Reversibility: yes
Reference: Long-range electrostatic interactions can influence the folding, stability, and cooperativity of dihydrofolate reductase. — Barmat L|Matthews C R|Onuffer J J|Perry K M|Gittelman M S (Biochemistry;28;7961-8, 1989)