Protein: Steroid Delta-isomerase (Pseudomonas putida)
UniProt: P07445
EC number: 5.3.3.1
Mutation: Y32S
PDB: 1OH0
Experiment dataset: ProTherm
Method: Urea
Measure: CD
pH: 7
Buffer: potassium phosphate 20 mM
Temperature: 25
dG: 10.6
ddG: -13.7
Cm: 1.56
m-value: 2.55
Reversibility: yes
Reference: Maintenance of alpha-helical structures by phenyl rings in the active-site tyrosine triad contributes to catalysis and stability of ketosteroid isomerase from Pseudomonas putida biotype B. — Nam G H|Jang D S|Cha S S|Lee T H|Kim D H|Hong B H (Biochemistry;40;13529-37, 2001)