Protein: Steroid Delta-isomerase (Pseudomonas putida)
UniProt: P07445
EC number: 5.3.3.1
Mutation: Y32F|V101L
PDB: 1OH0
Experiment dataset: ProTherm
Method: Urea
Measure: CD
pH: 7
Buffer: Sodium phosphate 25 mM
Temperature: 25
dG: 17.8
Cm: 4.45
m-value: 2.52
Reversibility: yes
Reference: Contribution of the hydrogen-bond network involving a tyrosine triad in the active site to the structure and function of a highly proficient ketosteroid isomerase from Pseudomonas putida biotype B. — Choi G|Nam G H|Jang D S|Kim D H|Oh B H|Kim J S|Ha N C|Kim M S (Biochemistry;39;4581-9, 2000)