Protein: 2K5N ()
Mutation: N53S|S58M
Experiment dataset: MegaScale
dG: 4.23059527043818
ddG: 0.21776937992831424
Trypsin: 1.62795785510432
Chymotrypsin: 0.4431787507329409
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)