Protein: Ribonuclease (Bacillus amyloliquefaciens)
UniProt: P00648
EC number: 3.1.27.-
Mutation: A90C|S127C
PDB: 1BNI
Experiment dataset: ProTherm
Method: Urea
Measure: Fluorescence
pH: 6.3
Buffer: MES 450 mM
Temperature: 25
dG: 10
ddG: -1.21
Cm: 5.77
m-value: 1.73
Reversibility: yes
Reference: Engineered disulfide bonds as probes of the folding pathway of barnase: increasing the stability of proteins against the rate of denaturation. — Clarke J|Fersht A R (Biochemistry;32;4322-9, 1993)