Protein: Thermonuclease (Staphylococcus aureus)
UniProt: P00644
EC number: 3.1.31.1
Mutation: V133I
PDB: 1STN
Experiment dataset: ProTherm
Method: GdnHCl
Measure: Fluorescence
pH: 7
Buffer: Sodium phosphate 25 mM
Temperature: 20
Ion: NaCl 100 mM
dG: 5.5
ddG: -0.1
Cm: 0.83
m-value: 6.54
Reversibility: yes
Reference: Energetics of side chain packing in staphylococcal nuclease assessed by exchange of valines, isoleucines, and leucines. — Chen J|Stites W E|Spencer D S|Byrne M P|Holder J B|Bennett A F (Biochemistry;40;13998-4003, 2001)