Protein: Thermonuclease (Staphylococcus aureus)
UniProt: P00644
EC number: 3.1.31.1
Mutation: V186S
PDB: 1STN
Experiment dataset: ProTherm
Method: GdnHCl
Measure: Fluorescence
pH: 7
Buffer: Sodium phosphate 25 mM
Temperature: 20
Ion: NaCl 100 mM
dG: 0.7
ddG: 4.9
Cm: 0.11
m-value: 0.87
Reversibility: yes
Reference: Energetic contribution of side chain hydrogen bonding to the stability of staphylococcal nuclease. — Stites W E|Byrne M P|Manuel R L|Lowe L G (Biochemistry;34;13949-60, 1995)