Protein: 2ROT ()
Mutation: D2F
Experiment dataset: MegaScale
dG: 3.333650465149795
Trypsin: 1.0264436616160566
Chymotrypsin: -0.028017279044961
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)