Protein: 1W4G ()
Mutation: E16M|A43F
Experiment dataset: MegaScale
dG: 2.909185055952968
ddG: 0.22581796875800153
Trypsin: 0.2381814695481801
Chymotrypsin: -0.2580050266988096
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)