Protein: Ribonuclease (Bacillus amyloliquefaciens)
UniProt: P00648
EC number: 3.1.27.-
Mutation: H65D
PDB: 1BNI
Experiment dataset: ProTherm
Method: Urea
Measure: Fluorescence
pH: 6.3
Buffer: MES 1.00 M
Temperature: 25
Ion: NaCl 613 mM
dG: 6.6
ddG: 2.2
Cm: 3.26
m-value: 2.04
Reversibility: yes
Reference: Alpha-helix stability in proteins. I. Empirical correlations concerning substitution of side-chains at the N and C-caps and the replacement of alanine by glycine or serine at solvent-exposed surfaces. — Serrano L|Sancho J|Fersht A R|Hirshberg M (J Mol Biol;227;544-59, 1992)