Protein: Dihydrofolate reductase (Escherichia coli (strain K12))
UniProt: P0ABQ4
EC number: 1.5.1.3
Mutation: G121L
PDB: 5DFR
Experiment dataset: ProTherm
Method: Urea
Measure: CD
pH: 7
Buffer: Potassium phosphate 10 mM
Temperature: 15
dG: 5.96
ddG: 0.38
Cm: 2.81
m-value: 2.12
Reversibility: yes
Reference: Effects of point mutation in a flexible loop on the stability and enzymatic function of Escherichia coli dihydrofolate reductase. — Gekko K|Yamagami K|Kunori Y|Ichihara S|Kodama M|Iwakura M (J Biochem;113;74-80, 1993)