Protein: 2K5P ()
Mutation: T21F|D50C
Experiment dataset: MegaScale
dG: 3.05402806543231
ddG: 0.52970585413357
Trypsin: 1.3168540222551377
Chymotrypsin: -0.0414227156038336
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)