Protein: Thermonuclease (Staphylococcus aureus)
UniProt: P00644
EC number: 3.1.31.1
Mutation: V148L|G170V
PDB: 1STN
Experiment dataset: ProTherm
Method: GdnHCl
Measure: Fluorescence
pH: 7
Buffer: Na3PO4 25 mM
Temperature: 20
Ion: NaCl 0.1 M
dG: 3.5
ddG: 2.1
Reversibility: yes
Reference: Stability mutants of staphylococcal nuclease: large compensating enthalpy-entropy changes for the reversible denaturation reaction. — Freire E|Shortle D|Meeker A K (Biochemistry;27;4761-8, 1988)