Protein: Guanyl-specific ribonuclease T1 (Aspergillus oryzae (strain ATCC 42149 / RIB 40))
UniProt: P00651
EC number: 4.6.1.24
Mutation: T117V|T119V
PDB: 1YGW|1RN1|9RNT
Experiment dataset: ProTherm
Method: Thermal
Measure: DSC
pH: 7
Buffer: MOPS 30000.0
Tm: 41.2
dTm: -11.3
ddG: 3.5
dH: 94
Reversibility: yes
State: 2.0
Reference: Contribution of hydrogen bonds to protein stability. — Takano Kazufumi|Scholtz J Martin|Pace C Nick|Trevino Saul R|Thurlkill Richard L|Fu Hailong|Lee Fryar Katrina|Landua John|Schell David|Imura Satoshi|Gajiwala Ketan|Sevcik Jozef|Urbanikova Lubica|Myers Jeffery K|Hebert Eric J|Shirley Bret A|Grimsley Gerald R (Protein Sci;23;652-61, 2014)