Protein: 2PFU ()
Mutation: T20D|T23Y
Experiment dataset: MegaScale
dG: 3.565584351232512
Trypsin: 1.1925694163362492
Chymotrypsin: 0.2097061872415506
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)