Protein: Subtilisin-chymotrypsin inhibitor-2A (Hordeum vulgare)
UniProt: P01053
Mutation: T59D|E61A
PDB: 2CI2
Experiment dataset: ProTherm
Method: GdnHCl
Measure: Fluorescence
pH: 6.3
Buffer: MES 450 mM
Temperature: 25
dG: 7.3
ddG: 0.24
Cm: 3.86
m-value: 1.89
Reversibility: yes
Reference: Contribution of residues in the reactive site loop of chymotrypsin inhibitor 2 to protein stability and activity. — Fersht A R|Jackson S E (Biochemistry;33;13880-7, 1994)