Protein: Guanyl-specific ribonuclease Sa3 (Kitasatospora aureofaciens)
UniProt: P30289
EC number: 4.6.1.24
Mutation: Y53F
PDB: 1MGR
Experiment dataset: ProTherm
Method: Thermal
Measure: CD
pH: 7
Buffer: MOPS 30 mM
Temperature: 25
ddG: 0.6
Reversibility: yes(>95 %)
Reference: Tyrosine hydrogen bonds make a large contribution to protein stability. — Horn G|Bechert J|Shaw K|Pace C N|Hebert E J (J Mol Biol;312;393-404, 2001)