Protein: Guanyl-specific ribonuclease Sa (Kitasatospora aureofaciens)
UniProt: P05798
EC number: 4.6.1.24
Mutation: E54Q
PDB: 1RGG
Experiment dataset: ProTherm
Method: Thermal
Measure: DSC
pH: 5.5
Buffer: sodium acetate, potassium chloride 0.01 M, 0.05 M
Tm: 47.7
dTm: -7.9
dH: 89
dHvH: 92
Reversibility: yes (90-95%)
Reference: Contribution of active site residues to the activity and thermal stability of ribonuclease Sa. — Pace C Nick|Yakovlev Gennady I|Mitkevich Vladimir A|Shaw Kevin L|Trevino Saul|Newsom Stephanie|Makarov Alexander A (Protein Sci;12;2367-73, 2003)