Protein: Cytochrome b5 (Bos taurus)
UniProt: P00171
Mutation: S23C|R52C
PDB: 1CYO
Experiment dataset: ProTherm
Method: Thermal
Measure: Fluorescence
pH: 7
Buffer: K2HPO4 5 mM
Tm: 44.8
dTm: -5.3
Reversibility: Yes
State: 2.0
Reference: Engineering out motion: introduction of a de novo disulfide bond and a salt bridge designed to close a dynamic cleft on the surface of cytochrome b5. — Daggett V|Storch E M|Atkins W M (Biochemistry;38;5054-64, 1999)