Protein: 1W4F ()
Mutation: T25H|D35E
Experiment dataset: MegaScale
dG: -0.23460410437889
ddG: -4.019008534407751
Chymotrypsin: -1.836886216615076
Stabilizing: False
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)