Protein: Subtilisin BPN' (Bacillus amyloliquefaciens)
UniProt: P00782
EC number: 3.4.21.62
Mutation: N325A
PDB: 1SUP|1SPB
Experiment dataset: ProTherm
Method: Thermal
Measure: DSC
pH: 8
Buffer: Tris-HCl 50 mM
Ion: CaCl2 10 mM
Tm: 75
dTm: -3.3
Reversibility: no
Reference: Proteases of enhanced stability: characterization of a thermostable variant of subtilisin. — Wood J|Pantoliano M W|Rollence M L|Bryan P N|Finzel B C (Proteins;1;326-34, 1986)