Protein: 1EKL ()
Mutation: S4N|T54A
Experiment dataset: MegaScale
Trypsin: -1.5022068052440751
Chymotrypsin: -2.4462956163290133
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)