Protein: Dihydrofolate reductase (Escherichia coli (strain K12))
UniProt: P0ABQ4
EC number: 1.5.1.3
Mutation: G121Y
PDB: 5DFR
Experiment dataset: ProTherm
Method: Thermal
Measure: CD
pH: 7
Buffer: Potassium phosphate 10 mM
Tm: 43.8
dTm: -5.5
dHvH: 88.4
Reversibility: no
Reference: Point mutations at glycine-121 of Escherichia coli dihydrofolate reductase: important roles of a flexible loop in the stability and function. — Gekko K|Kunori Y|Ichihara S|Kodama M|Takeuchi H (J Biochem;116;34-41, 1994)