Protein: Ribonuclease (Bacillus amyloliquefaciens)
UniProt: P00648
EC number: 3.1.27.-
Mutation: R116S|D140N
PDB: 1BNI
Experiment dataset: ProTherm
Method: Urea
Measure: Fluorescence
pH: 6.3
Buffer: MES 100 mM
Temperature: 25
ddG: 3.49
Cm: 2.76
m-value: 2.07
Reversibility: yes
Reference: Importance of two buried salt bridges in the stability and folding pathway of barnase. — Vuilleumier S|Fersht A R|Tissot A C (Biochemistry;35;6786-94, 1996)