Protein: 1GL5 ()
Mutation: E25F|K57A
Experiment dataset: MegaScale
dG: 2.7767074464799864
ddG: 0.18286322614395537
Trypsin: 0.2174358943687576
Chymotrypsin: -0.4084433520650182
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)