Protein: 2K5H ()
Mutation: S22F
Experiment dataset: MegaScale
dG: 1.83856548445257
ddG: 0.23682595450660515
Trypsin: 0.1325182210943624
Chymotrypsin: -1.0080864976235084
Reference: Mega-scale experimental analysis of protein folding stability in biology and design. — Tsuboyama Kotaro (Nature;620;434-444, 2023)