Modification of a SARS-CoV-2 spike-RBD targeting nanobody for pH-dependent binding and its chromatographic engineering for purification of WT and variant S-RBDs with mild elution conditions
Year: 2025
Type: article
Language: en
Topic: Monoclonal and Polyclonal Antibodies Research
Authors and affiliations:
raw_author_name: Xindan Zhang
raw_affiliation_strings: Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China
raw_author_name: Zimin Chen
raw_affiliation_strings: Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China
raw_author_name: Sheng Lin
raw_affiliation_strings: Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China
raw_author_name: Fanli Yang
raw_affiliation_strings: Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China
raw_author_name: Liyan Guo
raw_affiliation_strings: Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China; Department of Hematology, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China
raw_author_name: Jing Yang
raw_affiliation_strings: Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China
Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China
raw_author_name: Lingling Wang
raw_affiliation_strings: Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China
raw_author_name: Xin Yuan
raw_affiliation_strings: Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China
raw_author_name: Pengli Xu
raw_affiliation_strings: Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China
raw_author_name: Bin He
raw_affiliation_strings: Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China
raw_author_name: Yu Cao
raw_affiliation_strings: Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China; Disaster Medicine Center, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China
raw_author_name: Jian Li
raw_affiliation_strings: Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China
School of Basic Medical Sciences, Chengdu University, Chengdu, Sichuan, 610106, China
raw_author_name: Qi Zhao
raw_affiliation_strings: College of Food and Biological Engineering, Chengdu University, Chengdu, Sichuan, 610106, China
raw_author_name: Jiong Li
raw_affiliation_strings: Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China
School of Basic Medical Sciences, Chengdu University, Chengdu, Sichuan, 610106, China
raw_author_name: Li Yang
raw_affiliation_strings: Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China
raw_author_name: Wei Wang
raw_affiliation_strings: Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China
raw_author_name: Zhenling Wang
raw_affiliation_strings: Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China
raw_author_name: Jinliang Yang
raw_affiliation_strings: Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China
Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China
raw_author_name: Jingyun Yang
raw_affiliation_strings: Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China
Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China
raw_author_name: Guobo Shen
raw_affiliation_strings: Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China
raw_author_name: Xiawei Wei
raw_affiliation_strings: Laboratory of Aging Research and Cancer Drug Target, State Key Laboratory of Biotherapy and Cancer Center, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China
raw_author_name: Guangwen Lu
raw_affiliation_strings: Department of Emergency Medicine, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China. Electronic address: lugw@scu.edu.cn