LabID-base/OpenAlex-Afillation huggingface dataset:
Chemically-inducible CRISPR/Cas9 circuits for ultra-high dynamic range gene perturbation
Year: 2025
Type: article
Language: en
Topic: CRISPR and Genetic Engineering
Authors and affiliations:
raw_author_name: Rajini Srinivasan
raw_affiliation_strings: Department of Molecular Biology, Genentech Inc., South San Francisco, CA, USA
raw_author_name: Tao Sun
raw_affiliation_strings: Department of Molecular Biology, Genentech Inc., South San Francisco, CA, USA
raw_author_name: Azalia Sandles
raw_affiliation_strings: Department of Molecular Biology, Genentech Inc., South San Francisco, CA, USA
raw_author_name: Diana Wu
raw_affiliation_strings: Department of Functional Genomics, Genentech Inc., South San Francisco, CA, USA
raw_author_name: Liang Wang
raw_affiliation_strings: Department of Functional Genomics, Genentech Inc., South San Francisco, CA, USA
raw_author_name: Hetal Patel
raw_affiliation_strings: Department of Molecular Biology, Genentech Inc., South San Francisco, CA, USA
raw_author_name: Ryan Pabalate
raw_affiliation_strings: Department of Molecular Biology, Genentech Inc., South San Francisco, CA, USA
raw_author_name: Maram Bader
raw_affiliation_strings: Department of Molecular Biology, Genentech Inc., South San Francisco, CA, USA
raw_author_name: Amy Heidersbach
raw_affiliation_strings: Department of Molecular Biology, Genentech Inc., South San Francisco, CA, USA
raw_author_name: Clark Ho
raw_affiliation_strings: Department of Molecular Biology, Genentech Inc., South San Francisco, CA, USA
raw_author_name: Shiqi Xie
raw_affiliation_strings: Department of Functional Genomics, Genentech Inc., South San Francisco, CA, USA
raw_author_name: Andrew Ng
raw_affiliation_strings: Department of Molecular Biology, Genentech Inc., South San Francisco, CA, USA. ng.andrew.an1@gene.com
Department of Molecular Biology, Genentech Inc., South San Francisco, CA, USA
raw_author_name: Benjamin Haley
raw_affiliation_strings: Centre de Recherche de l'Hôpital Maisonneuve-Rosemont, Pavillon Claudine D'Amours, 5690 Boul. Rosemont, Montréal, H1T 2H2, QC, Canada. benjamin.haley@umontreal.ca
Department of Molecular Biology, Genentech Inc., South San Francisco, CA, USA. benjamin.haley@umontreal.ca
Department of Molecular Biology, Genentech Inc., South San Francisco, CA, USA
Development and Validation of the Artificial Intelligence in Mental Health Scale: Application for AI Mental Health Chatbots
Year: 2025
Type: article
Language: en
Topic: Digital Mental Health Interventions
Authors and affiliations:
raw_author_name: Aglaia Katsiroumpa
raw_affiliation_strings: Clinical Epidemiology Laboratory, Faculty of Nursing, National and Kapodistrian University of Athens, 10679 Athens, Greece
raw_author_name: Olympia Konstantakopoulou
raw_affiliation_strings: Clinical Epidemiology Laboratory, Faculty of Nursing, National and Kapodistrian University of Athens, 10679 Athens, Greece
raw_author_name: Ioannis Moisoglou
raw_affiliation_strings: Faculty of Nursing, University of Thessaly, 38241 Larissa, Greece
raw_author_name: Parisis Gallos
raw_affiliation_strings: Faculty of Nursing, University of West Attica, 12243 Egaleo, Greece
raw_author_name: Olga Galani
raw_affiliation_strings: Faculty of Nursing, University of West Attica, 12243 Egaleo, Greece
raw_author_name: Paschalina Lialiou
raw_affiliation_strings: Computational Biomedicine Research Lab, Department of Digital Systems, University of Piraeus, 18534 Piraeus, Greece
raw_author_name: Maria Tsiachri
raw_affiliation_strings: Clinical Epidemiology Laboratory, Faculty of Nursing, National and Kapodistrian University of Athens, 10679 Athens, Greece
raw_author_name: Petros Galanis
raw_affiliation_strings: Faculty of Nursing, University of Thessaly, 38241 Larissa, Greece
Factors Associated with Condomless Anal Sex and Absence of Pre-Exposure Prophylaxis (PrEP) Use Among Brazilian Men Who Have Sex with Men: A Cross-Sectional Study
Year: 2025
Type: article
Language: en
Topic: HIV/AIDS Research and Interventions
Authors and affiliations:
raw_author_name: Laelson Rochelle Milanês Sousa
raw_affiliation_strings: Nursing Course, State University of Maranhão, Coroata 65665-000, Brazil
raw_author_name: Patrícia Thais Cardoso da Silva
raw_affiliation_strings: Nursing Course, State University of Maranhão, Coroata 65665-000, Brazil
raw_author_name: Allan Araujo Rodrigues
raw_affiliation_strings: Nursing Course, State University of Maranhão, Coroata 65665-000, Brazil
raw_author_name: Márcio José dos Santos Silva
raw_affiliation_strings: Nursing Course, State University of Maranhão, Coroata 65665-000, Brazil
raw_author_name: José Carlos Vinícius Jansen de Paz
raw_affiliation_strings: Nursing Course, State University of Maranhão, Coroata 65665-000, Brazil
raw_author_name: Breno da Silva Oliveira
raw_affiliation_strings: Department of Nursing, Federal University of Mato Grosso do Sul, Coxim 79400-000, Brazil
raw_author_name: Daniel de Macêdo Rocha
raw_affiliation_strings: Department of Nursing, Federal University of Mato Grosso do Sul, Coxim 79400-000, Brazil
raw_author_name: Maria Wiklander
raw_affiliation_strings: Division of Nursing, Department of Neurobiology, Care Sciences and Society, Karolinska Institutet, 171-77 Stockholm, Sweden
raw_author_name: Elucir Gir
raw_affiliation_strings: Ribeirão Preto College of Nursing, University of São Paulo, Ribeirao Preto 14040-902, Brazil
raw_author_name: Renata Karina Reis
raw_affiliation_strings: Ribeirão Preto College of Nursing, University of São Paulo, Ribeirao Preto 14040-902, Brazil
Ciência Aberta nas Ciências biomédicas e nas Ciências da Saúde
Year: 2025
Type: article
Language: pt
Topic: Academic Publishing and Open Access
Authors and affiliations:
raw_author_name: Nancy Sánchez-Tarragó
raw_affiliation_strings: Universidade Federal do Rio Grande do Norte
raw_author_name: Taliane de Assis Oliveira
raw_affiliation_strings: Liga Norte Riograndense Contra o Câncer
Nerve Conduction Study and Functional Assessment After Upper Extremity Macroreplantation
Year: 2025
Type: article
Language: en
Topic: Nerve Injury and Rehabilitation
Authors and affiliations:
raw_author_name: Sławomir Kroczka
raw_affiliation_strings: Department of Child and Adolescent Neurology, Jagiellonian University Medical College, 30-663 Krakow, Poland
raw_author_name: Magdalena Jaworek
raw_affiliation_strings: Department of Child and Adolescent Neurology, Jagiellonian University Medical College, 30-663 Krakow, Poland
raw_author_name: Marta Lecznar-Piotrowska
raw_affiliation_strings: Malopolskie Center of Hand Rehabilitation (MCRR), Ludwik Rydygier Specialistic Hospital, 31-826 Krakow, Poland
raw_author_name: Małgorzata Steczkowska
raw_affiliation_strings: Department of Child and Adolescent Neurology, Jagiellonian University Medical College, 30-663 Krakow, Poland
raw_author_name: Anna Grela
raw_affiliation_strings: Department of Child and Adolescent Neurology, Jagiellonian University Medical College, 30-663 Krakow, Poland
raw_author_name: Aleksandra Gergont
raw_affiliation_strings: Department of Child and Adolescent Neurology, Jagiellonian University Medical College, 30-663 Krakow, Poland
A Brief Review of the Electromigration Reliability for Sn-Bi-Based Solder Joints
Year: 2025
Type: article
Language: en
Topic: Electronic Packaging and Soldering Technologies
Authors and affiliations:
raw_author_name: Jeongheon Lee
raw_affiliation_strings: Department of Mechanical System & Automotive Engineering, Chosun University, Gwangju 61452, Republic of Korea
raw_author_name: Jae B. Kwak
raw_affiliation_strings: Department of Mechanical Engineering, Chosun University, Gwangju 61452, Republic of Korea
Research on influencing factors and spatial heterogeneity of farmland net primary productivity based on the mobile-UNet model: a case study of Shanghai
Year: 2025
Type: article
Language: en
Topic: Land Use and Ecosystem Services
Authors and affiliations:
raw_author_name: Jiahao Li
raw_affiliation_strings: The Bartlett School of Planning, University College London
The Bartlett School of Planning, University College London, London, UK
raw_author_name: Jiaqi Zhang
raw_affiliation_strings: Manchester School of Architecture
Manchester School of Architecture, Manchester, UK
raw_author_name: Xuepeng Shi
raw_affiliation_strings: College of Architecture and Urban Planning, Qingdao University of Technology
College of Architecture and Urban Planning, Qingdao University of Technology, Qingdao, China
Factors Influencing the Willingness of Layer Specialized Households to Participate in Cooperative Avian Influenza Prevention and Control: Evidence from China
Year: 2025
Type: article
Language: en
Topic: Animal Disease Management and Epidemiology
Authors and affiliations:
raw_author_name: Donghao Guo
raw_affiliation_strings: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100000, China
raw_author_name: Hua Pu
raw_affiliation_strings: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100000, China
Assessment of 15 CMIP6 Models in Simulating the East Asian Winter Monsoon and Its Relationship with ENSO
Year: 2025
Type: article
Language: en
Topic: Climate variability and models
Authors and affiliations:
raw_author_name: Yiqiong Tang
raw_affiliation_strings: Climate Change and Resource Utilization in Complex Terrain Regions Key Laboratory of Sichuan Province, Chengdu University of Information Technology, Chengdu 610225, China
Key Laboratory of Ecosystem Carbon Source and Sink, China Meteorological Administration (ECSS-CMA), Wuxi University, Wuxi 214105, China
raw_author_name: Mengyu Li
raw_affiliation_strings: State Key Laboratory of Climate System Prediction and Risk Management/Key Laboratory of Meteorological Disaster, Ministry of Education/Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters, Nanjing University of Information Science and Technology, Nanjing 210044, China
Machine Learning Predictions of Deep Convection Initiation: The Importance of Geographic Information, the Sensitivity to Training Region, and Dominant Environmental Parameters
Year: 2025
Type: article
Topic: Meteorological Phenomena and Simulations
Authors and affiliations:
raw_author_name: Stephen A. Shield
raw_affiliation_strings: Department of Earth and Atmospheric Sciences, University of Nebraska–Lincoln, Lincoln, Nebraska
1University of Nebraska-Lincoln, Department of Earth and Atmospheric Sciences, Lincoln, NE
raw_author_name: Adam L. Houston
raw_affiliation_strings: Department of Earth and Atmospheric Sciences, University of Nebraska–Lincoln, Lincoln, Nebraska
1University of Nebraska-Lincoln, Department of Earth and Atmospheric Sciences, Lincoln, NE
Analysis of Legal Protection For Household Workers Without Employment Contracts
Year: 2025
Type: article
Topic: Indonesian Legal and Regulatory Studies
Authors and affiliations:
raw_author_name: Farrel Farandy
raw_affiliation_strings: Universitas Pembangunan Nasional “Veteran” Jakarta, Indonesia
raw_author_name: Wardani Rizkianti
raw_affiliation_strings: Universitas Pembangunan Nasional “Veteran” Jakarta, Indonesia
STRESS GRANULES AND α-SYNUCLEIN: INTERPLAY BETWEEN CELLULAR STRESS AND PROTEIN AGGREGATION IN PARKINSON’S DISEASE
Year: 2025
Type: article
Language: en
Topic: RNA Research and Splicing
Authors and affiliations:
raw_author_name: Share
raw_affiliation_strings: Stress granules (SGs) are membraneless organelles that are constituted primarily of untranslated messenger ribonucleoproteins, whose assembly occurs via liquid-liquid phase separation (LLPS) in response to various cellular stressors and that play a critical role in maintaining proteostasis. Increasing evidence indicates that chronic stress can disrupt the tightly regulated dynamics of SGs assembly and disassembly, leading to their persistence. These pathological SGs potentially serve as nucleation sites for aberrant protein aggregation in several neurodegenerative disorders (NDDs)2. Focusing on Parkinson’s disease (PD), current knowledge on the dysregulation of SGs assembly and disassembly in relation to α-Synuclein (α-Syn) aggregation is only based on cellular models, with no evidence from human brain tissue. It is well established that, under specific conditions, α-Syn can form condensates regulated by microtubule dynamics, promoting amyloid fibril formation via LLPS, and that α-Syn interacts with several RNA-binding proteins (RBPs) involved in SGs formation. Based on this we aim to determine whether: i) α-Syn pathology and SGs are associated in post-mortem human brains obtained from PD patients and ii) α-Syn aggregates within SGs under conditions of acute and chronic stress in a neuronal cell model (SH-SY5Y cells) and whether this process affects SGs homeostasis. Immunohistochemical analysis on post-mortem PD brains highlighted a characteristic pattern of small, round RBPs-positive granules in neuronal cell bodies, which also accumulated alongside α- Syn oligomers. Interestingly, different RBPs were found to be involved in Lewy body formation, and quantitative analyses showed a significant RBPs increase in mature compared to undefined aggregates. This suggests that SGs may act as an intermediate for α-Syn aggregation in PD brains. Simultaneously, SH-SY5Y cells exposed to both acute and chronic treatment with the oxidative stress inducer sodium arsenite showed an increase in intracellular aggregates positive for both SGs markers and α-Syn, indicating enhanced recruitment or association of α-Syn with SGs. Future work will aim to explore the impact of both α-Syn aggregation and microtubule dynamics on the homeostasis of SGs in PD pathology, providing further insight into the molecular mechanisms of the disease
SHORT-TERM EXPOSURE TO PARTICULATE MATTER TRIGGERS SELECTIVE MOLECULAR AND BEHAVIORAL ALTERATIONS IN A MOUSE MODEL OF MULTIPLE SCLEROSIS
Year: 2025
Type: article
Language: en
Topic: Air Quality and Health Impacts
Authors and affiliations:
raw_author_name: Share
raw_affiliation_strings: Multiple Sclerosis (MS) is a chronic disease of the Central Nervous System (CNS), where neuroinflammation and autoimmune response against myelin lead to functional impairments and psychiatric symptoms. Exposure to peaks of air pollution – and in particular of particulate matter (PM) - has been associated with increased hospitalizations for MS onset and relapses, pointing to persons with MS (or predisposed to develop MS) as a PM-vulnerable cohort. With the aim to disclose the biological substrate of such vulnerability, and to understand whether individuals primed to develop autoimmunity (as it occurs in MS and in the experimental autoimmune encephalomyelitis - EAE - animal model of MS) respond differently to PM, we firstly characterized plasmatic extracellular vesicles (EVs) and their microRNA (miRNA) cargo in healthy and presymptomatic EAE mice after exposure to PM10, compared to unexposed healthy and EAE mice. The response of EAE mice to PM10 did not differ in terms of EV number or source compared to that of healthy mice. Remarkable differences existed instead in the identity of deregulated EV-associated miRNAs, which in EAE mice were predicted to target several MS-relevant biological processes and nervous system-, immune- and inflammation- related pathways, possibly contributing to disease worsening. To validate our findings, we monitored EAE pathological course. PM10 exposure in the presymptomatic phase did not modify EAE course, as assessed by clinical and neuropathological analyses. Yet, in PM-exposed EAE mice we observed selective behavioral alterations - i.e. increased disinhibited, risk-taking and novelty-seeking behaviors - compatible with neurotransmitter imbalances linked with the detected EV-packaged miRNA deregulation. Overall, these data show a selective vulnerability of immunologically primed mice toward the effects of PM, detected at molecular and behavioral levels as early as in the pre-symptomatic stages of the pathology
THE ROLE OF RETINAL &lt;i&gt;mTOR&lt;/i&gt; IN RETINAL DEGENERATION
Year: 2025
Type: article
Language: en
Topic: Retinal Development and Disorders
Authors and affiliations:
raw_author_name: Share
raw_affiliation_strings: Retinal neurons are highly susceptible to oxidative stress produced by light-derived reactive oxygen species (ROS) and the metabolic demand generated by photo-transduction. This mostly occurs within retinal pigment epithelium (RPE), which sustains the metabolism of photo-transduction, and removes oxidized proteins, lipids and sugars from the outer segment of photoreceptors. Thus, RPE critically depends on mitochondrial turnover and removal of oxidized species by effective lysosomes. The present study, provides light and ultrastructural evidence about mTOR-dependency of retinal integrity with an emphasis on RPE cells. The mTOR activator 3-MA generates cell loss, which is prevented by the classic mTOR inhibitor rapamycin or the phytochemical curcumin, which possesses strong mTOR inhibiting effects. The loss of cell viability is associated with deficient mitochondrial turnover and accumulation of lipid droplets and glycogen granules. In these conditions typical proteins which form gap junctions (ZO1, occludin, RPE65) are suppressed and their low expression is confined to the perinuclear zone. This is relevant considering the role of these proteins in fostering the blood-retina barrier, endocytosis of photoreceptors, and phenotype of RPE. Moreover, the loss of these proteins, along with a damage to RPE cells and photoreceptors is a hallmark of early stages of age-related macular degeneration (AMD). Degeneration of RPE occurs early in AMD and further progresses towards inner retina involving bipolar, amacrine and ganglion cells. Inhibition of mTOR within RPE is critical to prevent the onset of degenerative changes. In this way, subsequent alterations such as the epithelial-mesenchymal transition, which mature later in AMD, is counteracted by mTOR suppression. Retinal protection induced by mTOR inhibition is concomitant with beclin1 expression. This may occur either as a consequence of pro-autophagy effects of mTOR inhibition or/and non-canonical autophagy-dependent effects of beclin1 activity. Retinal mTOR modulation appears a promising target to counteract retinal degeneration by preventing mitochondrial damage, lipid and glycogen accumulation
DISTRIBUTION, DENSITY, AND PHYLOGENETIC VARIATION OF CORTICAL IMMATURE NEURONS IN MACAQUES (RHESUS MONKEY)
Year: 2025
Type: article
Language: en
Topic: Neurogenesis and neuroplasticity mechanisms
Authors and affiliations:
raw_author_name: Share
raw_affiliation_strings: Brain structural plasticity, especially neurogenic processes, vary across species, anatomical regions, and ages. In addition to stem cell-driven adult neurogenesis, a form of “neurogenesis without division” has been recently shown, involving “immature” or “dormant” neurons in the cortical layer II (cortical immature neurons, cINs). These cells are prenatally generated and remain in a state of “arrested maturation”, retaining the ability to resume maturation at different life stages and functional integrate into pre-existing circuits. Previous quantification of cINs in widely different mammals showed high abundance of these cells in large-brained species with respect to rodents, and covariation with brain size and neocortical expansion. Here, using the same approach (immunocytochemical detection of the cytoskeletal protein doublecortin, after establishing correspondent neuroanatomical levels in each species), we characterized and quantified the cINs in macaques (Rhesus monkey), a primate with human-like brain anatomy, phylogenetics, and cognitive functions. First, we studied the cIN density in 4 young adult macaques (7-10 years) to understand their position in the phylogenetic variation. Our results place them among the gyrencephalic species and confirm the covariance between cIN density and both increased brain size and neocortical expansion. Then, we used 11 middle-aged macaques (23 years) to quantify the cIN density across 16 neuroanatomically defined cortical regions in search for possible regional variation. Significant differences were observed, with the highest presence in the medial and inferior temporal cortex. Overall, it is confirmed that cINs follow an evolutionary trend in mammals, involving the entire cortical mantle in gyrencephalic species, yet, with regional differences. Previous studies reported high amount of doublecortin-positive cells in the temporal lobe of humans, thus supporting the value of macaque as a good translational model for studying cINs
The Impacts of Large Language Model Addiction on University Students’ Mental Health: Gender as a Moderator
Year: 2025
Type: article
Language: en
Topic: Impact of Technology on Adolescents
Authors and affiliations:
raw_author_name: Ibrahim A. Elshaer
raw_affiliation_strings: Department of Management, School of Business, King Faisal University, Al-Ahsa 31982, Saudi Arabia
raw_author_name: Alaa M. S. A. Azazz
raw_affiliation_strings: Department of Social Studies, Arts College, King Faisal University, Al-Ahsa 31982, Saudi Arabia
&lt;i&gt;VGF&lt;/i&gt; AS BIOMARKER OF PRE-SYMPTOMATIC PHASE OF PARKINSON’S DISEASE
Year: 2025
Type: article
Language: en
Topic: Parkinson's Disease Mechanisms and Treatments
Authors and affiliations:
raw_author_name: Share
raw_affiliation_strings: Parkinson’s disease (PD) is a progressive neurodegenerative disorder whose early detection remains a major challenge. The Vgf gene, encoding the VGF neuroprotein, has been implicated in PD, with previous studies reporting reduced GF C-terminal peptide levels in the plasma of drug-naïve PD patients and in the substantia nigra (SN) of advanced PD models. However, similar studies, are lacking in progressive mouse models of PD. In this study, we developed a progressive and early PD mouse model using intranasal rotenone administration, based on the hypothesis that PD pathology begins in the olfactory bulb (OB) and progresses to the midbrain over time (Braak’s hypothesis). This study aimed to validate VGF C-terminal peptide levels as presymptomatic biomarkers of PD. Male BALB/c mice were divided into three cohorts based on the time of rotenone treatment, i.e., at 2, 3, and 6 months. Behavioral tests included the butyric acid avoidance to assess olfactory dysfunction and inverted grid and pole tests to assess motor impairment. At each time point, the mice were sacrificed, and brain and plasma were collected for q-PCR, immunohistochemistry (IHC) and ELISA. The total RNA was extracted from the isolated OB to perform q-PCR for the following genes: Vgf, Th (tyrosine hydroxylase), and Snca (synuclein alpha) while IHC was performed in OB and SN for TH, alpha-synuclein, and VGF C-terminal peptides. VGF C-terminal levels were measured using ELISA in plasma and OB extracted samples. Behavioral study showed significant olfactory deficits starting from 3-month time point and was persistent throughout the study, whereas motor impairments appeared only after 6 months of rotenone treatment. At 3 months, concurrent with olfactory deficits, using the OB samples, the VGF C-terminus levels were significantly reduced along with the increased immunoreactivity for alpha-synuclein, and decrease of TH. Same results for alpha-synuclein, TH and VGF were observed in the SN along with a decrease for VGF C-terminus levels in plasma. All these changes persisted even after 6 months, at which point gene alterations were observed for the first time. Indeed, the q-PCR results at 6 months showed increased Snca and decreased Th expression in the rotenone treated mice OB while Vgf mRNA levels remained unchanged in all brain regions suggesting a post-translational VGF changes. In conclusion, a role of VGF C-terminal peptides as a potential biomarker of pre-symptomatic PD is suggested