Anti-CRISPR-mediated continuous directed evolution of CRISPR-Cas9 in human cells
Year: 2025
Type: preprint
Language: en
Topic: CRISPR and Genetic Engineering
Authors and affiliations:
raw_author_name: Andrew L. Sabol
raw_affiliation_strings: Contributed equally to this work
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
Massachusetts Institute of Technology
raw_author_name: Amanuella A. Mengiste
raw_affiliation_strings: Contributed equally to this work
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
Massachusetts Institute of Technology
raw_author_name: Vedagopuram Sreekanth
raw_affiliation_strings: Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA
Contributed equally to this work
Broad Institute
raw_author_name: Prashant Singh
raw_affiliation_strings: Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA
Contributed equally to this work
Broad Institute
raw_author_name: Samuel J. Hendel
raw_affiliation_strings: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
Massachusetts Institute of Technology
raw_author_name: Minh Thuan Nguyen Tran
raw_affiliation_strings: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
Massachusetts Institute of Technology
raw_author_name: Anton M. Barybin
raw_affiliation_strings: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
Massachusetts Institute of Technology
raw_author_name: Santosh Chaudhary
raw_affiliation_strings: Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA
Broad Institute
raw_author_name: Ra’Mal M. Harris
raw_affiliation_strings: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
Massachusetts Institute of Technology
raw_author_name: Kristi Liivak
raw_affiliation_strings: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
Massachusetts Institute of Technology
raw_author_name: Zachary C. Severance
raw_affiliation_strings: Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA
Broad Institute
raw_author_name: Cale M. Locicero
raw_affiliation_strings: Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
Massachusetts Institute of Technology
raw_author_name: Karishma Kailass
raw_affiliation_strings: Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA
Broad Institute
raw_author_name: Chaiheon Lee
raw_affiliation_strings: Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA
Broad Institute
raw_author_name: Lucy Qinghua Xu
raw_affiliation_strings: Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA
Broad Institute
raw_author_name: Vincent L. Butty
raw_affiliation_strings: BioMicro Center, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
Massachusetts Institute of Technology
raw_author_name: Amit Choudhary
raw_affiliation_strings: Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA
Department of Medicine, Harvard Medical School, Boston, MA, 02115, USA
Divisions of Renal Medicine and Engineering, Brigham and Women's Hospital, Boston, Massachusetts, 02115, USA
Broad Institute
raw_author_name: Matthew D. Shoulders
raw_affiliation_strings: Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA
Massachusetts Institute of Technology