Development and validation of a flow cytometry-based ISG15 induction assay to monitor the type I interferon response
Year: 2025
Type: article
Language: en
Topic: interferon and immune responses
Authors and affiliations:
raw_author_name: Birthe Michiels
raw_affiliation_strings: Department of Laboratory Medicine, University Hospitals Leuven, Leuven, Belgium; Department of Microbiology, Immunology and Transplantation, Clinical and Diagnostic Immunology, KU Leuven, Leuven, Belgium. Electronic address: birthe.michiels@uzleuven.be
Unit of Molecular Signal Transduction in Inflammation, VIB-UGent Center for Inflammation Research, Belgium; Department of Biomedical Molecular Biology, Ghent University, Belgium. Electronic address: rudi.beyaert@irc.vib-ugent.be
raw_author_name: Rudi Beyaert
raw_affiliation_strings: Unit of Molecular Signal Transduction in Inflammation, VIB-UGent Center for Inflammation Research, Belgium; Department of Biomedical Molecular Biology, Ghent University, Belgium. Electronic address: rudi.beyaert@irc.vib-ugent.be
Unit of Molecular Signal Transduction in Inflammation, VIB-UGent Center for Inflammation Research, Belgium; Department of Biomedical Molecular Biology, Ghent University, Belgium; Department of Biochemistry and Microbiology, Ghent University, Belgium. Electronic address: jens.staal@irc.vib-ugent.be
raw_author_name: Jens Staal
raw_affiliation_strings: Unit of Molecular Signal Transduction in Inflammation, VIB-UGent Center for Inflammation Research, Belgium; Department of Biomedical Molecular Biology, Ghent University, Belgium; Department of Biochemistry and Microbiology, Ghent University, Belgium. Electronic address: jens.staal@irc.vib-ugent.be
Department of Microbiology, Immunology and Transplantation, Clinical and Diagnostic Immunology, KU Leuven, Leuven, Belgium. Electronic address: doreen.dillaerts@uzleuven.be
raw_author_name: Doreen Dillaerts
raw_affiliation_strings: Department of Microbiology, Immunology and Transplantation, Clinical and Diagnostic Immunology, KU Leuven, Leuven, Belgium. Electronic address: doreen.dillaerts@uzleuven.be
Department of Microbiology, Immunology and Transplantation, Clinical and Diagnostic Immunology, KU Leuven, Leuven, Belgium; PharmAbs, The KU Leuven Antibody Center, University of Leuven, Leuven, Belgium. Electronic address: maaike.cockx@uzleuven.be
raw_author_name: Maaike Cockx
raw_affiliation_strings: Department of Microbiology, Immunology and Transplantation, Clinical and Diagnostic Immunology, KU Leuven, Leuven, Belgium; PharmAbs, The KU Leuven Antibody Center, University of Leuven, Leuven, Belgium. Electronic address: maaike.cockx@uzleuven.be
Department of Laboratory Medicine, University Hospitals Leuven, Leuven, Belgium; Department of Microbiology, Immunology and Transplantation, Clinical and Diagnostic Immunology, KU Leuven, Leuven, Belgium. Electronic address: glynis.frans@uzleuven.be
raw_author_name: Glynis Frans
raw_affiliation_strings: Department of Laboratory Medicine, University Hospitals Leuven, Leuven, Belgium; Department of Microbiology, Immunology and Transplantation, Clinical and Diagnostic Immunology, KU Leuven, Leuven, Belgium. Electronic address: glynis.frans@uzleuven.be
Department of Laboratory Medicine, University Hospitals Leuven, Leuven, Belgium. Electronic address: birgit.timmermans@uzleuven.be
raw_author_name: Birgit Timmermans
raw_affiliation_strings: Department of Laboratory Medicine, University Hospitals Leuven, Leuven, Belgium. Electronic address: birgit.timmermans@uzleuven.be
Department of Respiratory Diseases, University Hospitals Leuven, Leuven, Belgium; Department of Chronic Diseases, Metabolism and Aging, BREATHE laboratory, KU Leuven, Leuven, Belgium. Electronic address: natalie.lorent@uzleuven.be
raw_author_name: Natalie Lorent
raw_affiliation_strings: Department of Respiratory Diseases, University Hospitals Leuven, Leuven, Belgium; Department of Chronic Diseases, Metabolism and Aging, BREATHE laboratory, KU Leuven, Leuven, Belgium. Electronic address: natalie.lorent@uzleuven.be
Department of Pediatrics, University Hospitals Leuven, Leuven, Belgium; Department of Microbiology, Immunology and Transplantation, Laboratory for Inborn Errors of Immunity, KU Leuven, Leuven, Belgium. Electronic address: isabelle.meyts@uzleuven.be
raw_author_name: Isabelle Meyts
raw_affiliation_strings: Department of Pediatrics, University Hospitals Leuven, Leuven, Belgium; Department of Microbiology, Immunology and Transplantation, Laboratory for Inborn Errors of Immunity, KU Leuven, Leuven, Belgium. Electronic address: isabelle.meyts@uzleuven.be
Department of Laboratory Medicine, University Hospitals Leuven, Leuven, Belgium; Department of Microbiology, Immunology and Transplantation, Clinical and Diagnostic Immunology, KU Leuven, Leuven, Belgium. Electronic address: xavier.bossuyt@uzleuven.be
raw_author_name: Xavier Bossuyt
raw_affiliation_strings: Department of Laboratory Medicine, University Hospitals Leuven, Leuven, Belgium; Department of Microbiology, Immunology and Transplantation, Clinical and Diagnostic Immunology, KU Leuven, Leuven, Belgium. Electronic address: xavier.bossuyt@uzleuven.be