Virus-like particles enable targeted gene engineering and pooled CRISPR screening in primary human myeloid cells
Year: 2025
Type: preprint
Language: en
Topic: CRISPR and Genetic Engineering
Authors and affiliations:
raw_author_name: Hyuncheol Jung
raw_affiliation_strings: Department of Medicine, University of California San Francisco, San Francisco, CA 94143, USA.;
Department of Medicine, University of California San Francisco, San Francisco, CA 9443, USA
raw_author_name: Pascal Devant
raw_affiliation_strings: Gladstone Institutes;
Gladstone Institutes
raw_author_name: Carter Ching
raw_affiliation_strings: Gladstone-UCSF Institute for Genomic Immunology, San Francisco, CA 94158, USA.;
Gladstone-UCSF Institute for Genomic Immunology, San Francisco, CA 94158, USA
raw_author_name: Mineto Ota
raw_affiliation_strings: Gladstone-UCSF Institute for Genomic Immunology, San Francisco, CA 94158, USA.;
Gladstone-UCSF Institute for Genomic Immunology, San Francisco, CA 94158, USA
raw_author_name: Jennifer Hamilton
raw_affiliation_strings: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA;
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA
raw_author_name: Zachary Steinhart
raw_affiliation_strings: Gladstone-UCSF Institute for Genomic Immunology, San Francisco, CA 94158, USA.;
Gladstone-UCSF Institute for Genomic Immunology, San Francisco, CA 94158, USA
raw_author_name: Wayne Ngo
raw_affiliation_strings: Gladstone Institute for Data Science and Biotechnology; San Francisco, CA 94158, USA;
San Francisco, CA 9418, USA
Gladstone Institute for Data Science and Biotechnology
raw_author_name: Luis Sandoval
raw_affiliation_strings: Department of Medicine, University of California San Francisco, San Francisco, CA 94143, USA.;
Department of Medicine, University of California San Francisco, San Francisco, CA 9443, USA
raw_author_name: Jae Hyung Jung
raw_affiliation_strings: Department of Medicine, University of California San Francisco, San Francisco, CA 94143, USA.;
Department of Medicine, University of California San Francisco, San Francisco, CA 9443, USA
raw_author_name: Da Xu
raw_affiliation_strings: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.;
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA
raw_author_name: Meirui An
raw_affiliation_strings: Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA.;
Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA
raw_author_name: Esha Urs
raw_affiliation_strings: Department of Medicine, University of California San Francisco, San Francisco, CA 94143, USA.;
Department of Medicine, University of California San Francisco, San Francisco, CA 9443, USA
raw_author_name: Peixin Amy Chen
raw_affiliation_strings: Department of Medicine, University of California San Francisco, San Francisco, CA 94143, USA.;
Department of Medicine, University of California San Francisco, San Francisco, CA 9443, USA
raw_author_name: Vincent Allain
raw_affiliation_strings: Department of Medicine, University of California San Francisco, San Francisco, CA 94143, USA.;
Department of Medicine, University of California San Francisco, San Francisco, CA 9443, USA
raw_author_name: Takuya Tada
raw_affiliation_strings: Department of Microbiology, NYU Grossman School of Medicine, New York, NY, 10016, USA
raw_author_name: James K Nuñez
raw_affiliation_strings: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.;
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA
raw_author_name: Nathaniel R Landau
raw_affiliation_strings: Department of Microbiology, NYU Grossman School of Medicine, New York, NY, 10016, USA
raw_author_name: David R. Liu
raw_affiliation_strings: Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA.;
Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA
raw_author_name: Justin Eyquem
raw_affiliation_strings: Department of Medicine, University of California San Francisco, San Francisco, CA 94143, USA.;
Department of Medicine, University of California San Francisco, San Francisco, CA 9443, USA
raw_author_name: Jennifer A. Doudna
raw_affiliation_strings: Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.;
Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA
raw_author_name: Alexander Marson
raw_affiliation_strings: Gladstone-UCSF Institute for Genomic Immunology, San Francisco, CA 94158, USA.;
Gladstone-UCSF Institute for Genomic Immunology, San Francisco, CA 94158, USA
raw_author_name: Julia Carnevale
raw_affiliation_strings: Department of Medicine, University of California San Francisco, San Francisco, CA 94143, USA.;
Department of Medicine, University of California San Francisco, San Francisco, CA 9443, USA