Calibration of Flow Cytometers Enables Reproducible Measurements of Extracellular Vesicle Concentrations and Reference Range Establishment
Year: 2025
Type: article
Language: en
Topic: Extracellular vesicles in disease
Authors and affiliations:
raw_author_name: Britta A. Bettin
raw_affiliation_strings: Amsterdam Vesicle Center, Amsterdam UMC Location University of Amsterdam  Amsterdam The Netherlands
Biomedical Engineering &amp; Physics, Amsterdam UMC Location University of Amsterdam  Amsterdam The Netherlands
Laboratory of Experimental Clinical Chemistry, Amsterdam UMC Location University of Amsterdam  Amsterdam The Netherlands
Laboratory of Experimental Clinical Chemistry, Amsterdam UMC, Location University of Amsterdam, Amsterdam, The Netherlands
raw_author_name: Bo Li
raw_affiliation_strings: Amsterdam Vesicle Center, Amsterdam UMC Location University of Amsterdam  Amsterdam The Netherlands
Department of Laboratory Medicine, Nanfang Hospital Southern Medical University  Guangzhou Guangdong China
Laboratory of Experimental Clinical Chemistry, Amsterdam UMC Location University of Amsterdam  Amsterdam The Netherlands
Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, China
Laboratory of Experimental Clinical Chemistry, Amsterdam UMC, Location University of Amsterdam, Amsterdam, The Netherlands
raw_author_name: Kim Falkena
raw_affiliation_strings: Exometry B.V.  Amsterdam The Netherlands
Exometry B.V., Amsterdam, The Netherlands
raw_author_name: Ton G. van Leeuwen
raw_affiliation_strings: Amsterdam Vesicle Center, Amsterdam UMC Location University of Amsterdam  Amsterdam The Netherlands
Biomedical Engineering &amp; Physics, Amsterdam UMC Location University of Amsterdam  Amsterdam The Netherlands
Biomedical Engineering & Physics, Amsterdam UMC, Location University of Amsterdam, Amsterdam, The Netherlands
raw_author_name: Christian Gollwitzer
raw_affiliation_strings: Physikalisch‐Technische Bundesanstalt (PTB)  Berlin Germany
Physikalisch-Technische Bundesanstalt (PTB), Berlin, Germany
raw_author_name: Zoltán Varga
raw_affiliation_strings: HUN‐REN Research Centre for Natural Sciences, Magyar tudósok körútja 2., H‐1117 Budapest, Hungary and Department of Physical Chemistry and Materials Science, Faculty of Chemical Technology and Biotechnology Budapest University of Technology and Economics  Budapest Hungary
HUN-REN Research Centre for Natural Sciences, Magyar tudósok körútja 2., H-1117 Budapest, Hungary and Department of Physical Chemistry and Materials Science, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Budapest, Hungary
raw_author_name: Nadine Ajzenberg
raw_affiliation_strings: Laboratoire d'Hématologie, AP‐HP Hôpital Bichat  Paris France
Université Paris Cité, INSERM U1148, LVTS  Paris France
Laboratoire d'Hématologie, AP-HP, Hôpital Bichat, Paris, France
Université Paris Cité, INSERM U1148, LVTS, Paris, France
raw_author_name: Jovan P. Antovic
raw_affiliation_strings: Coagulation, Clinical Chemistry Karolinska University Hospital  Stockholm Sweden
Department of Molecular Medicine &amp; Surgery Karolinska Institutet  Stockholm Sweden
Coagulation, Clinical Chemistry, Karolinska University Hospital, Stockholm, Sweden
Department of Molecular Medicine & Surgery, Karolinska Institutet, Stockholm, Sweden
raw_author_name: Pascale Berckmans
raw_affiliation_strings: Environmental Intelligence Unit Flemish Institute for Technological Research  Mol Belgium
Environmental Intelligence Unit, Flemish Institute for Technological Research, Mol, Belgium
raw_author_name: Edit I. Buzas
raw_affiliation_strings: Department of Genetics, Cell‐ and Immunobiology Semmelweis University  Budapest Hungary
HCEMM‐SU Extracellular Vesicle Research Group Semmelweis University  Budapest Hungary
HUN‐REN‐SU Translational Extracellular Vesicle Research Group Semmelweis University  Budapest Hungary
Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary
raw_author_name: Randy P. Carney
raw_affiliation_strings: Department of Biomedical Engineering University of California  Davis California USA
Department of Biomedical Engineering, University of California, Davis, California, USA
raw_author_name: Sean Cook
raw_affiliation_strings: Translational Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institutes National Institutes of Health  Bethesda Maryland USA
Translational Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institutes, National Institutes of Health, Bethesda, Maryland, USA
raw_author_name: Françoise Dignat‐George
raw_affiliation_strings: Aix Marseille Univ, INSERM 1263, INRAe 1260, C2VN  Marseille France
Department of Hematology, Biogenopole CHU La Timone, APHM  Marseille France
Department of Hematology, Biogenopole, CHU La Timone, APHM, Marseille, France
Aix Marseille Univ, INSERM 1263, INRAe 1260, C2VN, Marseille, France
raw_author_name: Dorothee Faille
raw_affiliation_strings: Laboratoire d'Hématologie, AP‐HP Hôpital Bichat  Paris France
Université Paris Cité, INSERM U1148, LVTS  Paris France
Laboratoire d'Hématologie, AP-HP, Hôpital Bichat, Paris, France
Université Paris Cité, INSERM U1148, LVTS, Paris, France
raw_author_name: Bernd Giebel
raw_affiliation_strings: Institute for Transfusion Medicine, University Hospital Essen University of Duisburg‐Essen  Essen Germany
Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany
raw_author_name: Jennifer C. Jones
raw_affiliation_strings: Translational Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institutes National Institutes of Health  Bethesda Maryland USA
Translational Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institutes, National Institutes of Health, Bethesda, Maryland, USA
raw_author_name: Yohan Kim
raw_affiliation_strings: Department of Urology Mayo Clinic  Rochester Minnesota USA
Department of Urology, Mayo Clinic, Rochester, Minnesota, USA
raw_author_name: Romaric Lacroix
raw_affiliation_strings: Aix Marseille Univ, INSERM 1263, INRAe 1260, C2VN  Marseille France
Department of Hematology, Biogenopole CHU La Timone, APHM  Marseille France
Aix Marseille Univ, INSERM 1263, INRAe 1260, C2VN, Marseille, France
Department of Hematology, Biogenopole, CHU La Timone, APHM, Marseille, France
raw_author_name: Joanne Lannigan
raw_affiliation_strings: Flow Cytometry Support Services, LLC USA
Flow Cytometry Support Services, LLC, USA
raw_author_name: Fabrice Lucien
raw_affiliation_strings: Department of Immunology Mayo Clinic  Rochester Minnesota USA
Department of Urology Mayo Clinic  Rochester Minnesota USA
Department of Urology, Mayo Clinic, Rochester, Minnesota, USA
raw_author_name: Katariina Maaninka
raw_affiliation_strings: EV group and EV core, Molecular and Integrative Biosciences Research Programme, Faculty of Biological and Environmental Sciences University of Helsinki Helsinki Finland
EV group and EV core, Molecular and Integrative Biosciences Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland
raw_author_name: Erika G. Marques de Menezes
raw_affiliation_strings: Department of Laboratory Medicine University of California  San Francisco California USA
Vitalant Research Institute  San Francisco California USA
Vitalant Research Institute, San Francisco, California, USA
Department of Laboratory Medicine, University of California, San Francisco, California, USA
raw_author_name: Annette Meyer
raw_affiliation_strings: European Center for Single Cell Solutions ESS BD Biosciences  Heidelberg Germany
European Center for Single Cell Solutions ESS, BD Biosciences, Heidelberg, Germany
raw_author_name: Rachel R. Mizenko
raw_affiliation_strings: Department of Biomedical Engineering University of California  Davis California USA
Department of Biomedical Engineering, University of California, Davis, California, USA
raw_author_name: Inge Nelissen
raw_affiliation_strings: Environmental Intelligence Unit Flemish Institute for Technological Research  Mol Belgium
Environmental Intelligence Unit, Flemish Institute for Technological Research, Mol, Belgium
raw_author_name: John Nolan
raw_affiliation_strings: Cellarcus Biosciences  La Jolla California USA
Scintillon Institute  San Diego California USA
Cellarcus Biosciences, La Jolla, California, USA
Scintillon Institute, San Diego, California, USA
raw_author_name: Philip J. Norris
raw_affiliation_strings: Department of Laboratory Medicine University of California  San Francisco California USA
Department of Medicine University of California  San Francisco California USA
Vitalant Research Institute  San Francisco California USA
Department of Laboratory Medicine, University of California, San Francisco, California, USA
Vitalant Research Institute, San Francisco, California, USA
raw_author_name: Desmond Pink
raw_affiliation_strings: Nanostics Inc. Edmonton  Alberta Canada
Vivian Triana, Nanostics Inc  Edmonton Alberta Canada
Vivian Triana, Nanostics Inc, Edmonton, Alberta, Canada
raw_author_name: Sumeet Poudel
raw_affiliation_strings: Biosystems and Biomaterials Division National Institute of Standards and Technology  Gaithersburg Maryland USA
Biosystems and Biomaterials Division, National Institute of Standards and Technology, Gaithersburg, Maryland, USA
raw_author_name: Stéphane Robert
raw_affiliation_strings: Aix Marseille Univ, INSERM 1263, INRAe 1260, C2VN, AMUTICYT Core Facility  Marseille France
Aix Marseille Univ, INSERM 1263, INRAe 1260, C2VN, AMUTICYT Core Facility, Marseille, France
raw_author_name: Pia R.‐M. Siljander
raw_affiliation_strings: EV group and EV core, Molecular and Integrative Biosciences Research Programme, Faculty of Biological and Environmental Sciences University of Helsinki Helsinki Finland
Finnish Red Cross Blood Service  Helsinki Finland
Finnish Red Cross Blood Service, Helsinki, Finland
EV group and EV core, Molecular and Integrative Biosciences Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Helsinki, Finland
raw_author_name: Vera A. Tang
raw_affiliation_strings: University of Ottawa Flow Cytometry &amp; Virometry Core Facility, Faculty of Medicine  Ottawa Canada
University of Ottawa Flow Cytometry & Virometry Core Facility, Faculty of Medicine, Ottawa, Canada
raw_author_name: Tobias Tertel
raw_affiliation_strings: Institute for Transfusion Medicine, University Hospital Essen University of Duisburg‐Essen  Essen Germany
Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen, Essen, Germany
raw_author_name: Tina Van Den Broeck
raw_affiliation_strings: Medical and Scientific Affairs BD Biosciences  Erembodegem Belgium
Medical and Scientific Affairs, BD Biosciences, Erembodegem, Belgium
raw_author_name: Lili Wang
raw_affiliation_strings: Biosystems and Biomaterials Division National Institute of Standards and Technology  Gaithersburg Maryland USA
Biosystems and Biomaterials Division, National Institute of Standards and Technology, Gaithersburg, Maryland, USA
raw_author_name: Joshua A. Welsh
raw_affiliation_strings: Advanced Technology Group BD Biosciences  San Jose California USA
Translational Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institutes National Institutes of Health  Bethesda Maryland USA
Translational Nanobiology Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institutes, National Institutes of Health, Bethesda, Maryland, USA
Advanced Technology Group, BD Biosciences, San Jose, California, USA
raw_author_name: Rienk Nieuwland
raw_affiliation_strings: Amsterdam Vesicle Center, Amsterdam UMC Location University of Amsterdam  Amsterdam The Netherlands
Laboratory of Experimental Clinical Chemistry, Amsterdam UMC Location University of Amsterdam  Amsterdam The Netherlands
Laboratory of Experimental Clinical Chemistry, Amsterdam UMC, Location University of Amsterdam, Amsterdam, The Netherlands
raw_author_name: Edwin van der Pol
raw_affiliation_strings: Amsterdam Vesicle Center, Amsterdam UMC Location University of Amsterdam  Amsterdam The Netherlands
Biomedical Engineering &amp; Physics, Amsterdam UMC Location University of Amsterdam  Amsterdam The Netherlands
Laboratory of Experimental Clinical Chemistry, Amsterdam UMC Location University of Amsterdam  Amsterdam The Netherlands
Laboratory of Experimental Clinical Chemistry, Amsterdam UMC, Location University of Amsterdam, Amsterdam, The Netherlands