A water-soluble two-photon fluorescence chemosensor for ratiometric imaging of mitochondrial viscosity in living cells
Zhao, Meng; Zhu, Yingzhong; Su, Jian; Geng, Qian; Tian, Xiaohe; Zhang, Jun; Zhou, Hongping; Zhang, Shengyi; Wu, Jieying; Tian, Yupeng; Journal of Materials Chemistry B 4 (2016) 5907–; doi:10.1039/C6TB01240J

data_7236433
_publ_section_title
;
 A water-soluble two-photon fluorescence chemosensor for ratiometric
 imaging of mitochondrial viscosity in living cells
;
_journal_issue                   35
_journal_name_full               'Journal of Materials Chemistry B'
_journal_page_first              5907
_journal_paper_doi               10.1039/C6TB01240J
_journal_volume                  4
_journal_year                    2016
_chemical_formula_moiety         'C27 H33 F6 N2 O4 P'
_chemical_formula_sum            'C27 H33 F6 N2 O4 P'
_chemical_formula_weight         594.52
_space_group_crystal_system      triclinic
_space_group_IT_number           2
_space_group_name_Hall           '-P 1'
_space_group_name_H-M_alt        'P -1'
_atom_sites_solution_hydrogens   geom
_audit_creation_method           SHELXL-2013
_audit_update_record
;
2016-01-25 deposited with the CCDC.
2016-07-29 downloaded from the CCDC.
;
_cell_angle_alpha                90.262(7)
_cell_angle_beta                 91.648(7)
_cell_angle_gamma                112.836(7)
_cell_formula_units_Z            2
_cell_length_a                   10.820(6)
_cell_length_b                   11.364(7)
_cell_length_c                   12.581(7)
_cell_measurement_reflns_used    1676
_cell_measurement_temperature    296(2)
_cell_measurement_theta_max      21.497
_cell_measurement_theta_min      2.207
_cell_volume                     1424.9(14)
_computing_cell_refinement       'Bruker SMART'
_computing_data_collection       'Bruker APEX2'
_computing_data_reduction        'Bruker SAINT'
_computing_molecular_graphics    'Bruker SHELXTL'
_computing_publication_material  'Bruker SHELXTL'
_computing_structure_refinement  'SHELXL-2013 (Sheldrick, 2013)'
_computing_structure_solution    'SHELXL-2013 (Sheldrick, 2013)'
_diffrn_ambient_temperature      296(2)
_diffrn_measured_fraction_theta_full 1.007
_diffrn_measured_fraction_theta_max 1.007
_diffrn_measurement_device_type  'Bruker Smart Apex CCD'
_diffrn_measurement_method       'phi and omega scans'
_diffrn_radiation_type           MoK\a
_diffrn_radiation_wavelength     0.71073
_diffrn_reflns_av_R_equivalents  0.0469
_diffrn_reflns_av_unetI/netI     0.0836
_diffrn_reflns_Laue_measured_fraction_full 1.007
_diffrn_reflns_Laue_measured_fraction_max 1.007
_diffrn_reflns_limit_h_max       12
_diffrn_reflns_limit_h_min       -12
_diffrn_reflns_limit_k_max       13
_diffrn_reflns_limit_k_min       -13
_diffrn_reflns_limit_l_max       15
_diffrn_reflns_limit_l_min       -15
_diffrn_reflns_number            10028
_diffrn_reflns_point_group_measured_fraction_full 1.007
_diffrn_reflns_point_group_measured_fraction_max 1.007
_diffrn_reflns_theta_full        25.242
_diffrn_reflns_theta_max         25.250
_diffrn_reflns_theta_min         1.619
_diffrn_source                   'fine-focus sealed tube'
_exptl_absorpt_coefficient_mu    0.171
_exptl_absorpt_correction_T_max  0.9666
_exptl_absorpt_correction_T_min  0.9504
_exptl_absorpt_correction_type   psi-scan
_exptl_absorpt_process_details   'SADABS; Bruker, 2000'
_exptl_crystal_colour            red
_exptl_crystal_density_diffrn    1.386
_exptl_crystal_density_method    'not measured'
_exptl_crystal_description       block
_exptl_crystal_F_000             620
_exptl_crystal_size_max          0.300
_exptl_crystal_size_mid          0.200
_exptl_crystal_size_min          0.200
_refine_diff_density_max         0.777
_refine_diff_density_min         -0.402
_refine_diff_density_rms         0.066
_refine_ls_extinction_method     none
_refine_ls_goodness_of_fit_ref   1.105
_refine_ls_hydrogen_treatment    constr
_refine_ls_matrix_type           full
_refine_ls_number_parameters     406
_refine_ls_number_reflns         5049
_refine_ls_number_restraints     185
_refine_ls_restrained_S_all      9.133
_refine_ls_R_factor_all          0.1751
_refine_ls_R_factor_gt           0.0829
_refine_ls_shift/su_max          0.001
_refine_ls_shift/su_mean         0.000
_refine_ls_structure_factor_coef Fsqd
_refine_ls_weighting_details
;
 w=1/[\s^2^(Fo^2^)+(0.1260P)^2^]
 where P=(Fo^2^+2Fc^2^)/3
;
_refine_ls_weighting_scheme      calc
_refine_ls_wR_factor_gt          0.2277
_refine_ls_wR_factor_ref         0.2624
_reflns_Friedel_coverage         0.000
_reflns_number_gt                2318
_reflns_number_total             5049
_reflns_threshold_expression     'I > 2\s(I)'
_cod_data_source_file            c6tb01240j2.cif
_cod_data_source_block           a
_cod_database_code               7236433
_shelx_estimated_absorpt_t_min   0.950
_shelx_estimated_absorpt_t_max   0.967
_shelxl_version_number           2013-2
_shelx_res_checksum              27767