Question: do all australian shepherds have different colored eyes

Answer: No, Also, great variety is seen in the Aussie's eye color, and they are often heterochromatic. An early nickname for the breed was ``ghost-eye dog''. Aussie eyes may be any shade of brown, or blue; they may have two differently colored eyes, or even have bicolored or ``split eyes'' (for example, a half-brown, half-blue eye), which appear to be linked to the merle coloration. Merled eyes occur, as well, where one color is mixed in and swirled with another. Any combination of eye color is acceptable in the breed standard, so long as the eyes are healthy. In general, however, black Aussies (self, bi-color, or tri-color) tend to have brown eyes, while red (self, bi-color, or tri-color) Aussies tend to have amber eyes, though these Aussies may also carry the blue-eyed gene.