biological therapy after chemotherapy in treating patients with relapsed or refractory non-hodgkin's lymphoma

Conditions: Leukemia; Lymphoma

Summary: rationale: biological therapies use different ways to stimulate the immune system and stop cancer cells from growing. drugs used in chemotherapy use different ways to stop cancer cells from dividing so they stop growing or die. combining biological therapy with chemotherapy may kill more cancer cells. purpose: phase i trial to study the effectiveness of biological therapy after chemotherapy in treating patients who have relapsed or refractory non-hodgkin's lymphoma.

objectives: primary * determine the safety and toxicity of cellular immunotherapy with autologous cd8+ cytotoxic t-lymphocyte clones after chemotherapy comprising cyclophosphamide, vincristine, and prednisone in patients with relapsed or refractory cd20+ indolent lymphomas or mantle cell lymphoma. secondary * determine the duration of in vivo persistence of adoptively transferred cd20-specific cd8+ cytotoxic t-lymphocyte clones in the absence and presence of subcutaneous interleukin-2 in these patients. * assess the trafficking of cd8+ cytotoxic t-lymphocyte clones to lymph nodes in these patients treated with this regimen. * determine immune response and tumor response in patients treated with this regimen. outline: this is an open-label, pilot study. * leukapheresis: patients undergo leukapheresis. selected cd20-specific cd8+ cells are cultured to expand the cytotoxic t lymphocytes (ctl), which are then cloned. * chemotherapy: patients receive oral cyclophosphamide and oral prednisone on days 1-5 and vincristine iv on day 1. courses repeat every 3-4 weeks for a total of 6 courses. * immune cell infusion: beginning 4 weeks after the last course of chemotherapy (and lymph nodes ≤ 5 cm diameter or ≤ 5,000 circulating cd20+ lymphocytes/mm\^3), patients receive autologous cd8+ ctl clones iv over 30 minutes. courses repeat every 2-5 days for a total of 3 courses in the absence of disease progression or unacceptable toxicity. the last 6 patients receive interleukin-2 subcutaneously every 12 hours for 14 days, beginning 2 hours after the last infusion of cd8+ ctl clones. after course 2 or 3 of immune cells, all patients undergo surgical lymph node biopsy to determine if immune cells are moving to the lymph nodes. patients are followed monthly for 1 year and then annually for 2 years. projected accrual: a total of 12 patients will be accrued for this study within 4 years.

Eligibility criteria:
disease characteristics: * immunohistopathologically documented relapsed or refractory cd20+ indolent lymphomas or mantle cell lymphoma * indolent b-cell lymphomas including any of the following subtypes: * follicular lymphoma (grade i, ii, or iii) * small lymphocytic lymphoma or chronic lymphocytic leukemia * marginal zone lymphoma (splenic, nodal, and extra-nodal) * lymphoplasmacytoid lymphoma * ineligible for or unwilling to participate in other fhcrc/uwmc protocols * serological evidence of prior exposure to epstein-barr virus * must agree to undergo peripheral blood drawing, bone marrow biopsy, lymph node biopsy, and nuclear medicine imaging * must agree to cytoreductive chemotherapy if necessary to reduce lymph nodes to \< 5 cm in diameter or circulating b lymphocyte counts to \< 5,000/mm\^3 * no pulmonary involvement * no cns involvement patient characteristics: age: * any age performance status: * not specified life expectancy: * at least 90 days hematopoietic: * not specified hepatic: * no active hepatitis b infection renal: * not specified other: * no hiv positivity * not pregnant or nursing * fertile patients must use effective contraception * no history of hypersensitivity reactions to murine proteins prior concurrent therapy: biologic therapy: * at least 4 months since prior rituximab, tositumomab, or ibritumomab * no prior allogeneic stem cell transplantation * no other concurrent immunotherapy (e.g., interferons, vaccines, or other cellular products) chemotherapy: * at least 2 years since prior fludarabine or cladribine * at least 4 weeks since prior chemotherapy and recovered endocrine therapy: * no concurrent systemic corticosteroids except to treat toxicity from chemotherapy or cellular immunotherapy radiotherapy: * not specified surgery: * not specified other: * at least 4 weeks since prior immunosuppressive therapy and recovered * no concurrent pentoxifylline * no other concurrent investigational agents

Keywords: recurrent grade 1 follicular lymphoma recurrent grade 2 follicular lymphoma recurrent grade 3 follicular lymphoma recurrent mantle cell lymphoma recurrent marginal zone lymphoma recurrent small lymphocytic lymphoma refractory chronic lymphocytic leukemia