Kornimate/medical-research-clean huggingface dataset:
non-invasive preimplantation genetic testing for aneuploidies using cell-free dna in spent culture media

Conditions: Secondary Infertility

Summary: analysis of embryonic cell-free dna (cfdna) present in the spent culture media (scm) is a non-invasive alternative for preimplantation genetic testing for aneuploidies (pgt-a) that avoids the technical challenges and limitations of biopsy. initial studies investigating this non-invasive pgt-a (nipgt-a) method reported variable concordance between cfdna in scm and the trophectoderm sample (\~ 30%-86%) and indicated a contribution from both the inner cell mass and trophectoderm to the cfdna in scm. this study aims to evaluate the use of the embryo culture medium as a source of genetic material for pgt-a and validate a nipgt-a protocol using cfdna in scm.

multiple studies have demonstrated the ability to detect and amplify cfdna from scm, at different stages of embryonic development, with varying rates of amplification success. differences in analytes, timing of scm collection and the duration of embryo culture within the collected medium, performance of assisted hatching (ah), whole genome amplification methods, comprehensive chromosome screening methods and next generation sequencing (ngs) platforms, bioinformatic analyses and strategies for identifying maternal contamination all contribute to the ultimate performance of nipgt-a. this study aims to validate a noninvasive pgt-a (nipgt-a) method utilizing cfdna released from the human blastocyst into the scm. patients undergoing a fertility treatment with pgt-a due to secondary infertility will be recruited. on day 6 post fertilization, scm will be collected prior to blastocyst biopsy. the scm is normally discarded at this stage. trophectoderm biopsy and sample collection will follow the ivf laboratory's standard practices for clinical pgt-a. three aneuploidy screening kits, relying on different whole genome amplification methods followed by ngs on the ion genestudio™ s5 prime system (thermofisher scientific), will be compared. the concordance between cfdna and trophectoderm biopsies will be evaluated for approximately 150 blastocysts with the best performing nipgt-a protocol. selection of the embryo(s) for transfer will be based solely on the pgt-a result from the biopsied cells. the patient's ivf+pgt-a treatment plan and timeline will not be altered.

Eligibility criteria:
inclusion criteria: * patients undergoing fertility treatment with pgt-a (recombinant fsh antagonist protocol with dual trigger) * secondary infertility * bmi 18- 35 kg/m2 * sperm: fresh ejaculated sperm (abstinence: 2-3 days) * at least one blastocyst biopsied on day 6 exclusion criteria: * high progesterone on day of trigger (\>1.5ng/ml) * vitrified oocytes * frozen sperm * indications for pgt-sr and pgt-m

Keywords: pgt-a cell-free dna spent culture media ngs
effects of mandibular exercises effect on pain perception and sensitivity in mandibular myofascial pain

Conditions: Temporomandibular Disorders

Summary: the effectiveness of mandibular exercises as a treatment of muscular orofacial pain is yet unknown. this study hypothesized that training exercises can be an effective therapy improving pain perception.

temporomandibular disorders (tmds) represent heterogeneous musculoskeletal disorders, where most common presentation is mandibular muscle pain, which is also more common among female than men. many interventions have been proposed for this pain control, regarding drugs, oral splints, relaxing exercises and self awareness but little is known about mandibular active contraction exercises. the aim of this study is to evaluate the effects of mandibular exercises in pain perception and sensitivity in women. a double blind randomized clinical trial will be conducted among fertile university student women with oral contraceptives (oc) use -to control hormonal influences-, with report of facial pain and myofascial pain diagnosis (rdc/tmd). they will be assessed in visual analogue scale (vas), sleep quality, pressure pain threshold (ppt) and pressure pain tolerance (pptol), and then allocated in one of the three interventions for 21 days: isometric mandibular active exercises over silicone bars; video-guided isotonic mandibular contractions over cloth pegs or counseling about disorders nature as passive comparison. after 21 days, vas, ppt and pptol in bilateral temporalis and masseter muscles will be assessed for a second time,) and compared with initial pain status. data will be entered by single blind operator, and will be open for quality auditing. site monitoring will be performed by the university clinical entities. data checks will be also performed as planned. descriptive and analytical statistics will be performed to address the primary and secondary objectives.

Eligibility criteria:
inclusion criteria: * temporomandibular myofascial pain diagnosis * oral contraceptives consumption * facial pain for at least 3 months (and at least 10 of the last 30 days at visit 0) * agrees to terms for continuing/discontinuing certain prescription/over-the-counter pain medications. exclusion criteria: * other orofacial pain diagnosis * oral surgery within 6 months * treatment for pain management within past 30 days * neurological or psychiatric disorders * orthodontic treatment * history of pregnancy, pregnancy or breastfeeding.

Keywords: musculoskeletal pain orofacial pain physical therapy modalities oral contraceptives mandibular diseases muscular diseases risk factors contraceptive usage pain, chronic craniomandibular disorders
in vivo study of thc-induced immune-genome changes at single cell solution in hiv-infected humans

Conditions: HIV Infections; Healthy

Summary: in this study, the investigators hypothesize that thc alters the immunogenome in a cell type-specific fashion and alters cytokine production via epigenetic regulatory mechanisms and that these alterations differ between hiv-infected and hiv-uninfected host genomes.

in this study, the investigators hypothesize that thc alters the immunogenome in a cell type-specific fashion and alters cytokine production via epigenetic regulatory mechanisms and that these alterations differ between hiv-infected and hiv-uninfected host genomes. to test these hypotheses, the investigators propose defining the epigenomic and transcriptomic alterations at single cell resolution in peripheral blood mononuclear cells by administering thc to humans with and without hiv infection. the thc-associated epigenomic/transcriptomic alterations will be linked to genomic variants to understand the causal effects of thc response in immune cells.

Eligibility criteria:
inclusion criteria (hiv-infected group): * hiv-positive * good medication adherence * negative urine toxicology for drugs of abuse (including cannabis) * good mental and physical health (other than hiv) as determined by history, psychiatric interview, collateral information, physical and laboratory examinations, ecg, and vital signs. inclusion criteria (hiv-uninfected group): * hiv-negative * negative urine toxicology for drugs of abuse (including cannabis) * good mental and physical health as determined by history, psychiatric interview, collateral information, physical and laboratory examinations, ecg, and vital signs. exclusion criteria (common to both groups): * major medical conditions, e.g., myocardial infarction, hypertension, etc. * positive pregnancy test and lactation; * weight greater than 166 kg (365 lbs)
goat milk infant formula comfort study

Conditions: Gastrointestinal Diseases; Cow's Milk Intolerance

Summary: in this study, the investigators aim to assess that infants with mild symptoms have significantly less (severe) symptoms after 14 days goat milk-based formula use compared to infants using cow's milk-based formula supplemented with probiotic drops.

anecdotal evidence shows that the use of goat milk-based infant formula decreases discomfort of infants with cow's milk related symptoms. in this randomized controlled pilot study, the effect on comfort during usage of goat-milk based infant formula is studied. twenty formula fed infants (aged between 6 weeks and 3 months) who experience mild discomfort will receive goat milk-based infant formula (gmf) or cow's mik based infant formula supplemented with probiotics lactobacillus reuteri drops (cmfp). the infants will be assessed by a local paediatricians using the cow's milk-related symptom score (comiss). this is a fast, non-invasive and easy-to-use and validated questionnaire to assess infant's reaction to proteins found in cow's milk. the higher the score, the more sensitive the child is to cow's milk proteins, the maximum score is 33 points. infants with a comiss score between 6 and 12 will be enrolled in this study. it is expected that infants will have a better response to gmf, which will result in a decrease of the comiss score after a 2 weeks intervention.

Eligibility criteria:
inclusion criteria: * healthy full-term infant with birth weight ≥2,500 to ≤4,500 g * infant aged between 4 weeks and 3 months at enrolment * comiss value at baseline between 6 and 12 * infant is exclusively bottle fed with cow's milk based infant formula at baseline exclusion criteria: * breastfed infants * congenital illness or malformation that may affect infant feeding or normal growth * gastrointestinal disorders * confirmed cow or goat milk protein allergy or lactose intolerance * supplemental feeding * readmission to hospital for longer than 3 days for another reason than hyperbilirubinemia prior to enrolment * infant participating in another clinical study
synthetic cbd as a therapy for covid-19

Conditions: Covid19; CBD

Summary: the study aim to test proof of concept of cbd treatment for efficacy and safety in patients suffering with mild covid-19 infection. the cbd will be delivered via oil droplets not containing thc, compared to placebo.


Eligibility criteria:
inclusion criteria: * covid 19 patients * 18 years and older exclusion criteria: * respiratory failure requiring mechanical ventilation * intensive care unit admission * neutrophile con. \< 1000 cells/mm3 * lymphocyte con \< 500 cells/mm3 * liver enzymes 5 times higher then the norm * qt interval longer then 500 ms. * pregnancy * hemodialysis renal replacement therapy * active or prior psychotic event
outcomes of bipolar electrocautery tonsillectomy versus cold steel dissection pediatric tonsillectomy

Conditions: Tonsillitis

Summary: it was intended to practically establish whether or not the relatively newer technique of tonsillectomy like bipolar electrocautery tonsillectomy has any added benefits in reducing the duration of surgery, blood loss and post-operative pain as compared with conventional cold steel dissection method. this study was done to compare the outcomes of bipolar electrocautery tonsillectomy versus cold steel dissection pediatric tonsillectomy.


Eligibility criteria:
inclusion criteria: * patients of 5-13 years of age * both genders * with a history of recurrent episodes of tonsillitis in the last year * children who had bilaterally enlarged tonsils * no history of fever or sore throat in the last 4 weeks exclusion criteria: * patients who had enlarged adenoids and required adenotonsillectomy * patients who underwent tonsillectomy after quinsy * with a history of bleeding disorders * patients who showed an international normalized ratio (inr) ≥ 1.5
impact of physical exercise on lymphedema related to breast cancer: assessment using thermography

Conditions: Breast Neoplasms; Lymphedema; Thermography; Exercise

Summary: study type: clinical trial primary purpose: to determine whether physical exercise improves or prevents lymphedema in breast cancer patients. description of the participant population/main condition: the study is aimed at women who have been diagnosed with breast cancer and have experienced post-treatment lymphedema or are at risk of developing it. the target population includes adults aged 18 to 70 who have been diagnosed with this condition following their breast cancer treatment. main research questions: does physical exercise improve or prevent lymphedema in women with breast cancer? what are the effects of physical exercise on the reduction of arm volume affected by lymphedema? what are the effects of physical exercise on the reduction of perceived disability in the arm affected by lymphedema? hypothesis: physical exercise intervention significantly reduces lymphedema symptoms, such as arm volume. physical exercise prevents the progression of lymphedema in women with breast cancer. comparison group: researchers will compare results between the experimental group (which will undergo physical exercise) and the control group (which will receive no intervention) to determine if physical exercise has a significant impact on the improvement or prevention of lymphedema. participant tasks: the experimental group will follow a supervised physical exercise program for a specific period. the control group will receive no intervention and will be monitored with standard follow-up care without exercise. participants will be evaluated before and after the study period to measure the progression of lymphedema, including arm volume measurement and surface skin temperature using thermography.


Eligibility criteria:
inclusion criteria: * women over 18 years old diagnosed with breast cancer. * residing in the province of león. * not currently undergoing any physical exercise intervention at the time of the study. * not participating in lifestyle modification programs at the time of the study. * not presenting any relative or absolute sequelae from chemotherapy treatment that would pose a relative or absolute contraindication to their participation in this intervention (e.g., cardiological sequelae). * having signed the informed consent to participate in the study. exclusion criteria: * presenting physical and/or psychological limitations that would hinder understanding or the ability to carry out the established intervention program. * presenting an infection in the upper limbs or lymphangitis. * fluid accumulation in the breast area requiring drainage at the time of the intervention. * not having signed the informed consent to participate in the study

Keywords: breast neoplasms lymphedema exercise thermography
evaluating the impact of year long, augmented diabetes self management support

Conditions: Diabetes Mellitus Type 2

Summary: improving diabetes social support is associated with health improvement benefits. this study is a prospective randomized controlled trial comparing the effects of telephone-based diabetes self-management support (dsms) compared with usual care, which includes diabetes self-management education (dsme), during a 1-year period. it will determine whether this specific model for providing on-going support to people with diabetes will better enable them to maintain the engagement in self-care behaviors, clinical improvements and diabetes-specific knowledge gained during the course of diabetes self-management education.

diabetes self management support is telephonic and will be provided by health navigators at a central call center

Eligibility criteria:
inclusion criteria: * english or spanish speaking * diagnosis of type 2 diabetes mellitus * hba1c \> 8 * have not completed a diabetes self management education program in the past year exclusion criteria: * current engagement in cancer treatment * diagnosis of end-stage renal disease * pregnant * receiving immunosuppressant therapy following transplant * diagnosis of serious mental illness * undergoing systemic treatment with prednisone

Keywords: diabetes self management education diabetes self management support type 2 diabetes
study to evaluate the safety of sebryl® and sebryl plus® in seborrheic dermatitis and psoriasis of scalp

Conditions: Seborrheic Dermatitis; Psoriasis of Scalp

Summary: observational, descriptive, retrospective, multicenter study to evaluate the safety of the treatment with sebryl® and / or sebryl plus® in the management of seborrheic dermatitis and psoriasis of the scalp in routine medical practice.

the objective of this study is to evaluate the frequency and intensity of adverse events presented during treatment with sebryl® and / or sebryl plus® for the management of seborrheic dermatitis and psoriasis of the scalp. the sample will be at convenience. the files of the patients who have received treatment with sebryl® and / or sebryl plus® in the last 5 years (2016 to 2021) will be chosen. the researchers or the personnel designated by them will capture the information recorded by the treating physicians in the clinical file, sociodemographic, clinical and safety data that were presented after the prescription of sebryl® and / or sebryl plus®.

Eligibility criteria:
inclusion criteria: * any sex. * treatment with sebryl® and / or sebryl plus® documented, for at least 2 consultations. * that the patient has been questioned about possible adverse events exclusion criteria: * that the patient has used some other concomitant treatment for seborrheic dermatitis and psoriasis on the scalp.

Keywords: allantoin coal tar clioquinol triclosan seborrheic dermatitis psoriasis of scalp
laparoscopic ileal interposition and weight regain

Conditions: Obesity

Summary: the investigators will study the ileal interposition to the treatment of weight regain after roux-en-y gastric bypass, assessing weight loss, body composition, quality of life, metabolic biomarkers, inflammatory biomarkers, and incretin hormones

this prospective, controlled, randomized intervention study will investigate the ileal interposition to the treatment of weight regain after roux-en-y gastric bypass, assessing weight loss, body composition, health-related quality of life, metabolic biomarkers, inflammatory biomarkers, and incretin hormones. the participants will also be assessed for alcohol abuse, depressive symptoms, level of physical activity, eating disorder symptoms, and dumping syndrome.

Eligibility criteria:
inclusion criteria: * patients submitted to initial laparoscopic after initial roux-en-y gastric bypass for at least 3 years * loss of excess weight between 60 to 80% * rate of weight regain ≥60% * body mass index ≥ 40 kg/m2 or ≥ 35 kg/m2, with at least 2 comorbidities associated with obesity * present a previous evaluation by the multidisciplinary team with a favorable opinion of the revision surgery. exclusion criteria: * active chemical dependency on alcohol and/or illicit drugs * severe psychotic or depressive disorder or history of suicide attempts in the last 12 months * eating behavior disorder (uncontrolled) * secondary causes of obesity * pregnancy * severe organ failure * active neoplastic * infectious or inflammatory disease * severe coagulopathy * high anesthetic-surgical risk * intellectual inability * lack of preoperative follow-up
application of real-time pcr platform for detection of vittaforma corneae among patients with microsporidia keratitis

Conditions: Microsporidia Keratitis; Microsporidial Keratoconjunctivitis

Summary: diagnosis of patients with microsporiosis relies on pathological findings as well as laboratory detection of the causative organism. the conventional laboratory diagnosis of microsporiosis relies on microscopic visualization of the characteristic v. corneae organisms. we develop a fully automated molecular platform for detection of vittaforma corneae among patients with microsporidia keratitis.

clinical infections due to microsporidium spp. are protean and can be found in humans, insects, and fishes. the most common type of infection is keratitis and mostly is caused by vittaforma corneae. diagnosis of patients with microsporiosis relies on pathological findings as well as laboratory detection of the causative organism. the conventional laboratory diagnosis of microsporiosis relies on microscopic visualization of the characteristic v. corneae organisms. laboratory tools of identification of v. corneae include gram staining, giemsa staining, and modified ziehl-neelsen staining of scratching or biopsied specimens of infected cornea. however, the sensitivity of these staining methods is not acceptable due to the variation of specimen quality and quantity, processing, and low load of organisms in the specimens. the bd max system (beckon dickinson, diagnostic systems, sparks, md, usa) was introduced in clinical microbiology laboratory of ntuh in 2014 for pneumocystis jirovecii detection. till now, no data are available in using this system on v. corneae detection. we develop a fully automated molecular platform for detection of vittaforma corneae among patients with microsporidia keratitis.

Eligibility criteria:
inclusion criteria: patients with suspected microsporidia infection in eye exclusion criteria:
photodynamic therapy with visudyne for human retinoblastoma: a preliminary study

Conditions: Retinoblastoma

Summary: to study the effectiveness and safety profiles of photodynamic therapy (pdt) for the treatment of human retinoblastoma.

retinoblastoma is the commonest primary intraocular malignancy in infancy and early childhood. ophthalmologist are trying to seek other more safe and effective treatment modalities to well control the tumor and preserve the visual function. photodynamic therapy (pdt) is a potentially therapeutic approach for retinoblastoma which has been underestimated. in this study, we are gonging to investigate the the effectiveness and safety profiles of photodynamic therapy (pdt) for the treatment of human retinoblastoma.

Eligibility criteria:
inclusion criteria: * patients with intraocular retinoblastoma of group a-d exclusion criteria: * (1) patients with intraocular retinoblastoma of group e; (2) patients with extraocular spread; (3); media opacities such as corneal opacity, significant cataract, or vitreous hemorrhage obscuring fundus examination; (4) patients who were allergic to verteporfin or other benzoporphyrin derivatives.

Keywords: photodynamic therapy retinoblastoma visudyne verteporfin
emerging invasive fungal infections in critically ill patients

Conditions: Fungal Infection; ICU Acquired Weakness

Summary: rationale: beside candida and aspergillus, emerging invasive fungal infections (eifis) are increasing in intensive care setting and are associated with high morbidity and mortality. however, data are scarce, particularly in icu settings and for eifis other than mucormycosis. objectives: to describe epidemiological trends and clinical features of eifis in intensive care units (icu) and to assess their outcome. methods: all records of adult patients diagnosed with an eifi in a medical icu between 2006 and 2019 were analyzed retrospectively. in-icu mortality was assessed, then factors associated with mortality were identified. survival at day-90 was calculated by the kaplan-meier method.


Eligibility criteria:
inclusion criteria: - adults (≥ 18 years old) with at least 1 positive culture for molds other than candida and aspergillosis exclusion criteria: - age \< 18, pregnancy

Keywords: emergind invasive fungal infection mucormycosis icu