atrial fibrillation and characterization of blood platelet

Conditions: Atrial Fibrillation

Summary: arterial thromboembolism constitutes a major risk of atrial fibrillation (af) requiring antithrombotic therapy. platelets and microparticles (mps) are important for hemostasis and thrombosis, their role during af is not well known. the principal objective of this study is to compare morphologic, functional and proteomics characteristics of blood platelet between af patients and healthy volunteers.

af is associated with decreased quality of life and an increased risk of thromboembolic events. af is the leading cause of embolic stroke. identification of factors that may influence blood coagulability in these patients could better identify therapeutic targets to optimize anticoagulation. platelets play a major role in the coagulation process, their study may provide valuable information. moreover microparticles from platelet activation are known to have pathophysiological effects including effects on thrombosis and inhibition of fibrinolysis. it has also been shown that the microparticles have a role in endothelial dysfunction and generation of inflammatory condition which are associated with atrial fibrillation. investigators propose in this project, a comparative study between af patients and healthy volunteers. complete characterization of platelet (morphology, platelet function, platelet proteome) and microparticles levels will be perform. different site blood sampling (systemic or cardiac) will be done in patients during their ablation procedure. patients will be followed during their hospitalisation.

Eligibility criteria:
inclusion criteria: * inclusion criteria: valid for all groups * age superior or equal to 18 years old, both genders. * patient affiliated or recipient of a social welfare regimen. * patient's write agreement for study participation after reading information note * inclusion criteria: specific for groups group 1: * volunteers without heart disease. * volunteers who never had af and not in af the day of inclusion. group 2 * patient suffering of paroxysmal atrial fibrillation since at least 6 month and addressed for primo ablation of atrial fibrillation. * patient in sinus rhythm the day of inclusion. group 3 * patient suffering of paroxysmal atrial fibrillation since at least 6 month and addressed for primo ablation of atrial fibrillation. * patient in atrial fibrillation the day of inclusion group 4 * patient suffering of persistent atrial fibrillation since at least 6 month and addressed for primo ablation of atrial fibrillation. * patient in sinus rhythm the day of inclusion. group 5 * patient suffering of persistent atrial fibrillation since at least 6 month and addressed for primo ablation of atrial fibrillation. * patient in atrial fibrillation the day of inclusion exclusion criteria: * age \<18 years. * active smoker (\> 10 cigarettes/days) * pregnant woman or breastfeeding women or not receiving effective contraception. * volunteer participating in another interventional study requiring taking drug. * volunteer having taken antinflammatory, platelet anti-aggregant or calcium-channel blocker drugs within 8 days prior to inclusion * valvular heart diseases. * chronic inflammatory diseases. * cardiovascular event or stroke within 3 month prior to inclusion * uncontrolled hypertension * chronic hepatic or renal diseases.

Keywords: atrial fibrillation, blood platelet