il-17 neutrophils in cf lung inflammation

Conditions: Cystic Fibrosis

Summary: the purpose of this study is to determine whether il-17 polymorphonuclear leukocytes (pmns) are central to the disease pathology in cf. this will be determined by demonstrating that il-17 pmns are present in the cf airway, correlate with lung function measures, and decrease in patients being treated with iv antibiotics for a pulmonary exacerbation.

this study consists of two parts which will be conducted in parallel. in the first part of the study, 14 subjects will be recruited for the clinically stable cohort. subjects will be asked to provide 1 gm of expectorated sputum and 40-ml of blood. a cell count and differential will be performed on the sputum followed by analysis for il-17 pmns by fluorescence-activated cell sorter (facs). il-17 pmns also will be isolated by running the remainder of the cell pellet through a column of magnetic beads designed for this purpose. these neutrophils will be lysed and intracellular cytokines determined. sputum supernatants will be stored frozen until analyzed for the presence of il-1β, il-6, il-8, il-17a, tgf-β, tnf-α, and neutrophil elastase. clinical data will be captured from the subject's clinical outpatient visit including lung function measures and clinical culture results. il-17 pmns will be correlated with lung function measures and inflammatory mediators at baseline. in the second part of this study, 10 subjects will be recruited for the exacerbation cohort. subjects will provide at least 1 gram of expectorated sputum and 40-ml of blood within 72 hours of hospital admission. sputum and blood will be processed and analyzed as described above. sputum and blood also will be obtained at the end of treatment (within 72 hours of completion of iv antibiotics) when the subject is at his or her baseline as determined by the managing physician. clinical data will be captured from the subject's hospitalization records including lung function measures and clinical culture results.

Eligibility criteria:
inclusion criteria: * for both cohorts: ≥ 18 \< 50 years of age * for both cohorts: must have a documented diagnosis of cf (positive sweat chloride ≥60 milliequivalents (meq)/liter, by pilocarpine iontophoresis) and/or a genotype with two identifiable mutations consistent with cf accompanied by one or more clinical features with the cf) phenotype * for both cohorts: chronically infected with p. aeruginosa defined by 2 positive cultures in the past year or 3 in the last two years * for both cohorts: ability to expectorate mucus * for both cohorts: ability to provide written informed consent * for clinically stable cohort: 4 subjects with one copy of g551d * for clinically stable cohort: 10 subjects who do not have g551d, they must have one copy of f508del * for clinically stable cohort: cf subjects must have a baseline fev1 percent predicted \> 50% (in the last year, obtained from medical record) * for clinically stable cohort: clinically stable: free of any acute illness for \>14 days * for clinically stable cohort: must have performed spirometry for clinical purposes at that clinical visit * for clinically stable cohort: must have a sputum culture sent to the clinical lab for clinical purposes at the time of the study visit * for clinically stable cohort: have not been prescribed any new systemic antibiotics for the 14 days prior to enrollment * for exacerbation/iv antibiotics cohort: one copy of f508del * for exacerbation/iv antibiotics cohort: must have a doctor defined pulmonary exacerbation requiring treatment with iv antibiotics * for exacerbation/iv antibiotics cohort: must have performed spirometry for clinical purposes within 72 hours of initiating iv antibiotics and within 72 hours of completing iv antibiotics * for exacerbation/iv antibiotics cohort: must have a sputum culture sent to the clinical lab for clinical purposes within 72 hours of admission exclusion criteria: * for both cohorts: pregnancy (based on self-report) * for both cohorts: co-infection with burkholderia cepacia complex organisms * for both cohorts: any condition that in the opinion of the subject or the subject's managing physician that would compromise that individuals ability to participate in these studies * for both cohorts: inability to tolerate the study procedures

Keywords: cystic fibrosis il-17 neutrophils