peripheral stem cell transplant, white blood cell infusions, chemotherapy, and radiation therapy in treating patients with recurrent metastatic cervical or vaginal cancer

Conditions: Cervical Cancer; Vaginal Cancer

Summary: rationale: giving low doses of chemotherapy, such as fludarabine, and radiation therapy before a donor peripheral blood stem cell transplant helps stop the growth of cancer cells. it also stops the patient's immune system from rejecting the donor's stem cells. the donated stem cells may replace the patient's immune system and help destroy any remaining cancer cells (graft-versus-tumor effect). giving an infusion of the donor's t cells (donor lymphocyte infusion) after the transplant may help increase this effect. sometimes the transplanted cells from a donor can also make an immune response against the body's normal cells. giving cyclosporine and mycophenolate mofetil after the transplant may stop this from happening. purpose: this phase ii trial is studying how well donor peripheral stem cell transplant plus chemotherapy and total-body irradiation followed by donor white blood cell infusion work in treating patients with recurrent metastatic or locally advanced cancer of the cervix or vagina that is associated with human papillomavirus.

objectives: primary * determine the partial or complete response in patients with recurrent metastatic or locally advanced human papillomavirus (hpv)-associated cervical or vaginal carcinoma treated with a nonmyeloablative regimen comprising fludarabine and low-dose total body irradiation followed by allogeneic peripheral blood stem cell transplantation, cyclosporine, mycophenolate mofetil, and donor lymphocyte infusion. secondary * determine the toxicity of this regimen in these patients. * determine whether this regimen induces engraftment and donor chimerism in these patients. * determine the hpv-e6 and hpv-e7 specific t-cell responses in selected patients treated with this regimen. outline: this is a pilot study. patients receive conditioning therapy comprising fludarabine iv on days -4 to -2 and low-dose total body irradiation on day 0. filgrastim (g-csf)-mobilized allogeneic peripheral blood stem cells are infused on day 0. patients also receive oral cyclosporine twice daily on days -3 to 35 and then tapered until day 56. mycophenolate mofetil is administered orally twice daily on days 0-27. patients with disease progression and no graft-versus-host disease on day 56 receive nonmobilized donor lymphocyte infusion (dli) over 30 minutes on day 65. dli may be repeated every 65 days for up to 4 doses. patients are followed weekly for 3 months, monthly for 6 months, every 6 months for 2 years, and then annually for 5 years. projected accrual: a total of 10 patients will be accrued for this study.

Eligibility criteria:
disease characteristics: * histologically confirmed recurrent metastatic or locally advanced cervical or vaginal carcinoma that is not curable with surgery or radiotherapy * tumor is human papillomavirus positive by polymerase chain reaction * bidimensionally measurable disease by clinical examination or radiographic imaging * availability of an genotypically hla-identical sibling donor (excluding identical twins) * no brain metastases patient characteristics: age: * under 65 performance status: * karnofsky 80-100% life expectancy: * not specified hematopoietic: * not specified hepatic: * bilirubin no greater than 2 times upper limit of normal (uln) * sgot and sgpt no greater than 2 times uln renal: * creatinine clearance at least 40 ml/min cardiovascular: * cardiac ejection fraction at least 40% * no history of congestive heart failure * no poorly controlled hypertension pulmonary: * no severe defects in pulmonary function * no supplementary continuous oxygen other: * not pregnant or nursing * fertile patients must use effective contraception during and for 12 months after study completion * hiv negative prior concurrent therapy: biologic therapy: * concurrent growth factors for severe persistent or febrile neutropenia after transplantation allowed chemotherapy: * not specified endocrine therapy: * not specified radiotherapy: * see disease characteristics surgery: * see disease characteristics

Keywords: stage iii cervical cancer recurrent cervical cancer stage ivb cervical cancer stage iva cervical cancer stage iii vaginal cancer stage iva vaginal cancer stage ivb vaginal cancer recurrent vaginal cancer