pilot trial of sirolimus/mec in high risk acute myelogenous leukemia (aml)

Conditions: AML

Summary: the purpose of this study is to evaluate the addition of sirolimus (rapamycin) to standard chemotherapy for the treatment of patients with high risk acute myelogenous leukemia (aml). cancer cells taken from the patients will be studied in the laboratory to see if rapamycin is affecting the mtor pathway in the cells and if this effect is correlated with how well patients respond to the therapy.

recent improvements in our understanding of leukemia biology have led to the introduction of highly effective, molecularly targeted therapies. this is exemplified by the development of bcr-abl tyrosine kinase inhibitors such as imatinib as monotherapy for chronic myeloid leukemia (cml) and in combination with chemotherapy for bcr-abl+ acute lymphoblastic leukemia (all). imatinib mesylate blocks the protein made by the bcr-abl oncogene. the pi3k (phosphatidylinositol 3-kinases) signaling is critical to leukemia cell survival and can be targeted. growth and survival stimulating signal transduction pathways are abnormally and universally activated in aml (acute myeloid leukemia). this signal cascade is thought to contribute to survival and growth in tumor cells via downstream effects upon target proteins akt/protein kinase b and mammalian target of rapamycin (mtor) a protein that helps control several cell functions. in aml, we and others have shown that pi3k signaling is constitutively activated in over 85% of primary samples and that the small molecule pi3k inhibitor ly294002 is cytotoxic in vitro to virtually all samples tested. as ly294002 is poorly suited for drug development, we have concentrated upon other ways to inhibit signal transduction through this pathway. mammalian target of rapamycin (mtor) emerged as a reasonable target due to the availability of clinically available, highly specific inhibitors with favorable safety profiles. mammalian target of rapamycin (mtor) plays a central but complex role in cancer cells' metabolic regulation and survival. this serine/threonine kinase coordinates several important cellular functions and its activity is modulated in response to amino acid, glucose, oxygen, and atp availability as well as extracellular growth factor ligation. mammalian target of rapamycin (mtor) activity regulates protein translation, nutrient and amino acid uptake, mitochondrial respiration, glycolysis, cell size regulation, cell cycle entry and progression, ribosome biogenesis, and autophagy. constitutive mammalian target of rapamycin (mtor) activation is commonly seen in cancer cells and is thought to promote survival in the setting of a wide variety of cellular insults. importantly, mtor opening may cause chemotherapy resistance. although regulation of mtor signaling in leukemia occurs through by several inputs, mtor activity in aml is thought to be primarily regulated by pi3k signaling through akt via the agent tumor suppressor tuberous sclerosis complex (tsc1\& 2) and its target rheb gtpase. taken together, mammalian target of rapamycin mtor is a smart target for molecularly targeted therapy in aml due to its importance in the growth and survival of aml cells, its necessity for aml cell survival in certain contexts, and its probable role in chemotherapy resistance and relapse.

Eligibility criteria:
inclusion criteria: * patients must have histologic evidence of high risk acute myeloid leukemia defined as one of the following: 1. primary refractory non-m3 aml (i) residual leukemia after a minimum of 2 prior courses of chemotherapy (same or different) (ii) evidence of leukemia after a nadir bone marrow biopsy demonstrates no evidence of residual leukemia. 2. relapsed non-m3 aml 3. any non-m3 aml age \>60 with no evidence of favorable karyotype (stratum 2 only), defined by presence of t(8;21)(q22;q22) \[aml1-eto\], inv16(p13;q22), or t(16;16)(p13;q22) \[cbf;myh11\] by cytogenetics, fish, or rt-pcr 4. secondary aml (from antecedent hematologic malignancy or following therapy with radiation or chemotherapy for another disease) with no evidence of favorable karyotype (stratum 2 only), defined by presence of t(8;21)(q22;q22) \[aml1-eto\], inv16(p13;q22), or t(16;16)(p13;q22) \[cbf;myh11\] by cytogenetics, fish, or rt-pcr * age \> or = 18 * ecog = 0 or 1 exclusion criteria: * subjects with fab m3 (t(15;17)(q22;q21)\[pml-rar\]) are not eligible * subjects taking the following are not eligible: * carbamazepine (e.g., tegretol) * rifabutin (e.g., mycobutin) or * rifampin (e.g., rifadin) * rifapentine (e.g., priftin) * st. john's wort * clarithromycin (e.g., biaxin) * cyclosporine (e.g. neoral or sandimmune) * diltiazem (e.g., cardizem) * erythromycin (e.g., akne-mycin, ery-tab) * itraconazole (e.g., sporanox) * ketoconazole (e.g., nizoral) * telithromycin (e.g., ketek) * verapamil (e.g., calan sr, isoptin, verelan) * voriconazole (e.g., vfend) * tacrolimus (e.g. prograf) * subjects taking fluconazole, voriconazole, itraconazole, posaconazole, and ketoconazole within 72 hours of study entry are not eligible. reinstitution of fluconazole, voriconazole, itraconazole, posaconazole, ketoconazole and diltiazem is permissible 72 hours after the last dose of sirolimus. * subjects must not be receiving any chemotherapy agents (except hydroxyurea). intrathecal methotrexate and cytarabine are permissible * subjects must not be receiving growth factors, except for erythropoietin

Keywords: aml mtor rapamycin