mineralocorticoid receptor (mr) antagonist (eplerenone) vs amlodipine and striatin

Conditions: Hypertension; Genetics Hypertension

Summary: salt sensitivity of blood pressure is a substantial risk factor for cardiovascular morbidity and mortality. inappropriate increases in renal sodium reabsorption lead to volume expansion, hypertension and salt sensitive blood pressure. key homeostatic mechanisms that regulate renal sodium reabsorption are: first, hormonal, e.g., renin-angiotensin-aldosterone system and second, vascular, e.g., renal vasculature. dysfunction in one or both mechanisms leads to hypertension and salt sensitive blood pressure. the investigators recently documented that striatin plays a novel role in the development of salt sensitive blood pressure. however, the mechanisms that lead to striatin-mediated salt sensitive blood pressure are not clear; defining these mechanisms is the overall goal of this proposal. striatin is a calmodulin- and caveolin-binding protein that can function as either a scaffolding and/or signaling protein, specifically in relation to the mechanism of action of steroids. in a large study of well characterized subjects from the international hypertensive pathotype (hyperpath) cohort, the investigators documented that hypertensive and normotensive humans who are striatin risk allele carriers have salt sensitive blood pressure. the investigators then developed a striatin heterozygous knockout mouse as a tool to identify potential mechanisms for the salt sensitive blood pressure. the investigators documented that these mice also have salt sensitive blood pressure with higher blood pressure levels and inappropriately increased aldosterone levels on a liberal salt diet.

hypothesis: hypertensive striatin risk allele carriers will show significantly greater reductions in blood pressure with a specific aldosterone mediated treatment approach (mineralocorticoid receptor blockade) than with a non-specific approach (amlodipine). protocol: 1. the screened, eligible hypertensives will enter a two to three-week single blind placebo washout phase. pill count will be used to determine compliance. those with blood pressure between 145-170/90-109 mmhg and pill count between 80-100% after two to three-weeks will enter the randomized phase and counseled regarding salt intake. 2. the first step: subjects will be fed a 10 meq na+, 100 meq k+ calculated diet for 6 days. the meals will be provided by the cci dietary core. on the 7th day, the subjects will come to the cci ambulatory clinical center. after remaining supine for 60-90 minutes, the subjects will have blood samples obtained for future analyses and their blood pressure measured using an automatic recording sphygmomanometer (space labs, snoqualmie, wa). readings will be obtained every 2 minutes for 20 minutes with the highest and lowest values discarded and the rest averaged. from the morning of the 6th to the morning of the 7th day, a 24-hr. urine will be collected for creatinine and sodium as a check on balance and stored for future analyses. 3. the second step: subjects will be counseled regarding liberal salt dietary intake. subjects will be given high salt broth packets, calcium tablets (as calcium bicarbonate), and potassium tablets (as potassium bicarbonate) to supplement their diet to ensure similar intakes in all subjects \[na+ (200 meq), potassium (k+, 100 meq) and calcium (800 mg)\]. this or a greater level of na+ intake was consumed by 60-70% of subjects before entering the hyperpath protocol. on the morning of the 6th day the subject will begin a 24-hour blood pressure recording, using a blood pressure machine provided by study staff. after completion of this diet for 6 days, the subject will come to the center for clinical investigation (cci) ambulatory clinical center and after remaining supine for 60-90 mins will have blood samples obtained for future analyses, and their bp measured using an automatic recording sphygmomanometer (space labs, snoqualmie, wa). readings will be obtained every 2 mins for 20 mins with the highest and lowest values discarded and the rest averaged. from the morning of the 6th to the morning of the 7th day, a 24-hr. urine will be collected for creatinine and na+ as a check on balance and stored for future analyses. the bp data from 2) and 3) will allow us to calculate salt sensitivity of blood pressure (ssbp), an approach performed successfully more than 500 times in the hyperpath cohort. 4. the hypertensive striatin risk carrier subjects will be randomized, double blinded to one of two primary treatment groups and titrated to effect every four weeks, as was previously reported (1, 2), eplerenone 50, 100 and 200 mg daily or amlodipine 2.5, 5 and 10 mg daily. study duration will be sixteen weeks. 5. participants will be randomized in blocks of four to 1 of 2 study drugs (amlodipine and eplerenone). the drug class assignment will remain the same throughout the study for each subject. the pi or co-pi will notify the brigham and womens hospital investigational drug pharmacy service to escalate or maintain current dose on each of the two visits. the brigham and womens hospital investigational drug pharmacy service will be responsible for the randomization schema, recording of study drug assignment, and dispensing of prescriptions. all others (pi, co-pi, research staff, subject) will be blinded as to drug assignment. 6. home seated, morning cuff blood pressure and pulse will be obtained daily and monitored by study staff weekly as a safety check with the information provided to the study staff by email or phone. the blood pressure results from the 7 days prior to the next visit will be averaged, with the highest and lowest values discarded. this average blood pressure value will be used to determine if the drug dose will be titrated after the next visit if the subject has reached goal blood pressure (less than or equal to140/90 mmhg), then the subject is maintained on his/her current dose. if not, the subject's dose is up titrated as per protocol. a member of the research team will contact the subject if the blood pressure data are not received within 24-hr of the designated time. if the cuff blood pressure is greater than 170/109 mmhg on two consecutive occasions 24 hours apart, or the subject develops cardiovascular symptoms, the subject will be terminated from the study and re-started on medications used before the washout period. the subject's physician will be contacted. 7. because eplerenone may increase serum k+ levels, we will monitor its level monthly during the study. this will be obtained at the time of their monthly visit unless a dose increase is made at the time of the visit. then, the serum k+ will be obtain one week later. if serum k+ is greater than 5.5 millimolar (mm) on two consecutive occasions 24 hours apart, the subject will be terminated from the study. if unexpected adverse event is noted the brigham and women's hospital investigational drug pharmacy service will be notified so as to break code and identify agent. 8. at the end of four weeks since randomization and the subjects have completed the first dose of the agent, the subject will be counseled regarding a 24-hour dietary intake of sodium (200 meq), potassium (100 meq) and calcium (800 mg). subjects will receive supplementary high salt broth packets to ensure that they are reaching the target na+ intake. after completion of this diet for 6 days, the subject returns to center for clinical investigation - ambulatory clinical center and after remaining supine for 60-90 minutes again will have blood samples obtained for future analyses and their blood pressure measured using an automatic recording sphygmomanometer. readings will be obtained every 2 minutes for 20 minutes with the highest and lowest values discarded and the rest averaged. this measurement will be used to assess the blood pressure response to a single dose of each agent. from the morning of the 6th to the morning of the 7th day, a 24-hr. urine will be collected for creatinine and sodium as a check on balance and stored for future analyses. 9. home seated, morning cuff blood pressure and pulse will be obtained daily and monitored by study staff weekly as a safety check with the information provided to the study staff by email or phone. the blood pressure results from the 7 days prior to the next visit will be averaged, with the highest and lowest values discarded. this average blood pressure value will be used to determine if the drug dose will be titrated after the next visit if the subject has reached goal blood pressure (less than or equal to140/90 mmhg), then the subject is maintained on his/her current dose. if not, the subject's dose is up titrated as per protocol. a member of the research team will contact the subject if the blood pressure data are not received within 24-hr of the designated time. if the cuff blood pressure is greater than 170/109 mmhg on two consecutive occasions 24 hours apart, or the subject develops cardiovascular symptoms, the subject will be terminated from the study and re-started on medications used before the washout period. the subject's physician will be contacted. 10. because eplerenone may increase serum k+ levels, we will monitor its level monthly during the study. this will be obtained at the time of their monthly visit unless a dose increase is made at the time of the visit. then, the serum k+ will be obtain one week later. if serum k+ is greater than 5.5 mm on two consecutive occasions 24 hours apart, the subject will be terminated from the study. if unexpected adverse event is noted the brigham and women's hospital investigational drug pharmacy service will be notified so as to break code and identify agent. 11. at the end of eight weeks since randomization and the subjects have completed the second dose of the agent, the subject will be counseled regarding a 24-hour dietary intake of sodium (200 meq), potassium (100 meq) and calcium (800 mg). subjects will receive supplementary high salt broth packets to ensure that they are reaching the target na+ intake. after completion of this diet for 6 days, the subject returns to center for clinical investigation - ambulatory clinical center and after remaining supine for 60-90 minutes again will have blood samples obtained for future analyses and will have their blood pressure measured using an automatic recording sphygmomanometer. readings will be obtained every 2 minutes for 20 minutes with the highest and lowest values discarded and the rest averaged. from the morning of the 6th to the morning of the 7th day, a 24-hr. urine will be collected for creatinine and sodium as a check on balance and stored for future analyses. 12. home seated, morning cuff blood pressure and pulse will be obtained daily and monitored by study staff weekly as a safety check with the information provided to the study staff by email or phone. a member of the research team will contact the subject if the blood pressure data are not received within 24-hr of the designated time. if the cuff blood pressure is greater than 170/109 mmhg on two consecutive occasions 24 hours apart, or the subject develops cardiovascular symptoms, the subject will be terminated from the study and re-started on medications used before the washout period. the subject's physician will be contacted. 13. because eplerenone may increase serum k+ levels, we will monitor its level monthly during the study. this will be obtained at the time of their monthly visit unless a dose increase is made at the time of the visit. then, the serum k+ will be obtain one week later. if serum k+ is greater than 5.5 mm on two consecutive occasions 24 hours apart, the subject will be terminated from the study. if unexpected adverse event is noted the brigham and women's hospital investigational drug pharmacy service will be notified so as to break code and identify agent. 14. at the end of the study, twelve weeks after randomization, subjects will be counseled regarding liberal salt dietary intake. subjects will be given high salt broth packets, calcium tablets (as calcium bicarbonate), and potassium tablets (as potassium bicarbonate) to supplement their diet to ensure similar intakes in all subjects \[na+ (200 meq), potassium (k+, 100 meq) and calcium (800 mg)\]. this or a greater level of na+ intake was consumed by 60-70% of subjects before entering the hyperpath protocol. on the morning of the 6th day the subject will begin a 24-hour blood pressure recording, using a blood pressure machine provided by study staff. after completion of this diet for 6 days, the subject will come to the center for clinical investigation (cci) ambulatory clinical center and after remaining supine for 60-90 mins will have blood samples obtained for future analyses, and their bp measured using an automatic recording sphygmomanometer (space labs, snoqualmie, wa). readings will be obtained every 2 mins for 20 mins with the highest and lowest values discarded and the rest averaged. from the morning of the 6th to the morning of the 7th day, a 24-hr. urine will be collected for creatinine and na+ as a check on balance and stored for future analyses. 15. the subject then will have completed the study and will be re-started on medications used before the washout period. the subject's physician will be contacted.

Eligibility criteria:
the subjects evaluated in this protocol are hypertensive and carry the striatin rs254093 risk allele or both striatin rs888083 and rs6744560 risk alleles. most will be recruited from the hyperpath cohort. the hyperpath cohort was developed under a specialized center of research (scor) in hypertension program. this program has demographic data and dna on more than 4000 subjects. currently, nearly 2000 of them have undergone an extensive phenotyping protocol. all hypertensive medications have been stopped for 4 weeks before study except agents that interfere with the renin-angiotensin-aldosterone system (raas) are stopped for three months and amlodipine and/or hydrochlorothiazide is added if necessary for blood pressure control until one month before study initiation. then each subject is studied twice on a diet consisting of 100 mmol potassium, 800 mg calcium, isocaloric, 2500 ml. the two times they are studied are after one week of a liberal sodium diet (200 mmol) and after one week of a low sodium diet (10 mmol). blood is obtained supine, upright and after a 3 ng ang ii infusion and a norepinephrine dose response curve. bp and renal plasma flow are assessed in response to the diet and the ang ii infusion and 24 hour urines are collected on each diet. an oral glucose tolerance test is performed on each subject and blood is obtained for dna. in addition to hypertensives, the nearly 2000 intensively studied cohort consists of 75 individuals in 10 families, 225 sibling pairs with hypertension, 525 normotensive individuals without a family history of hypertension or diabetes before the age of 60, and 250 type ii diabetic subjects with or without hypertension. on most of the 4000 subjects serum, plasma, urine and dna is available for measurements and analyses. currently the data set consists of approximately 2100 data points of demographic data, family history, biomarkers, and genotypes. the subjects have been recruited from boston, massachusetts, salt lake city,ance, rome, italy, and nashville utah, paris, fr, tenessee. the demographics consists of the following: 52% male, 18% of african descent, 3% asian descent, age 17-66, hypertension stage 1-2, diet or oral medication controlled diabetes (80% of the total diabetics). a few subjects will be recruited from advertisements on the internet and in local newspapers, from fliers and postings in the hospital, through mailings to households located in the boston areas and through patient registries at brigham and womens hospital. as an example of the richness of these sources is the research patient data registry (rpdr). rpdr is a centralized clinical data registry of 2.8 million brigham and womens hospital and massachusetts general hospital patients. with approval of the institutional review board, investigators may use the rpdr data acquisition engine to obtain medical record information for patients with a specific diagnosis. patients who meet inclusion criteria for a specific study can be identified. potential research subjects may be contacted by his/her physician to inform the patient of the possibility of participating in a research study and to provide the patient with the information for contacting the study personnel. investigators from brigham and womens hospital may apply to use the rpdr. rpdr contains over 90,000 hypertensives, ages 17-65 years with 13.5 % of african descent and 52.8 % women. we reported in our studies using the hyperpath cohort that there was no racial, age or ethnic differences in the salt sensitive blood pressure responses related to striatin allele variants. thus, an equal number of females and males and the same proportion of africans as in the hyperpath cohort will be studied. subjects in hyperpath and those recruited for the new study in this project will have the similar characteristics. the range in age is \>17; however, it is anticipated that the clear majority will be between the ages of 40 and 60 years. hypertensive patients previously treated will be weaned off medications for two-four weeks except agents that interfere with the renin-angiotensin-aldosterone system (raas) are stopped for three months and amlodipine and/or hydrochlorothiazide is added if necessary for blood pressure control until one month before study initiation. thus, these subjects will match the characteristics of subjects recruited in hyperpath. they must have a diastolic blood pressure between 95 and 105 mm hg off medication in each of three screening visits. subjects with diastolic blood pressures greater than 105 mm hg or systolic blood pressures greater than 180 mm hg will be excluded. subjects with only elevated systolic blood pressure (but diastolic less than 95 mm hg) will be excluded because such subjects were not in the hyperpath cohort. based on individual statements, subjects with current excessive alcohol use (greater than 12 oz/ethanol/week) or recreational drug use will be excluded. subjects taking other medications (except thyroid supplements) or weighing more than 150% of an ideal body weight will be excluded. subjects with other major cardiovascular diseases, diabetes, asthma, or other major medical illness will be excluded. subjects who smoke will be excluded. in addition, subjects must have normal values for the following screening tests: complete blood cell count (cbc), serum electrolytes, liver enzymes, thyroid stimulating hormone (tsh), urinalysis, 24-hour urine excretion of catecholamines and cortisol, and ecg. specifically, estimated glomerular filtration rate (gfr) must be \> 60 ml/min and serum potassium \< 5.0 mmol/l. subjects with hypokalemia while on diuretics will be evaluated for hyperaldosteronism before inclusion in this study. cushings syndrome will be ruled out clinically, and with a 24-hour urine cortisol if there is clinical uncertainty. for the more difficult question of renal artery stenosis, we will perform renal artery digital subtraction angiogram in patients with hypertension and a two-component abdominal bruit. patients with greater than 50% renal artery stenosis will be further evaluated, but excluded from this study. subjects with a known sensitivity to any of the agents, such as amlodipine or eplerenone will be excluded. women who are pregnant will be excluded and will be dropped from the study if they become pregnant during the study because eplerenone has not been approved for use in pregnancy and the activity of the raas is dramatically altered by pregnancy. the screened, eligible hypertensives will enter a two-week single blind placebo washout phase. pill count will be used to determine compliance. those with bp between 145-170/90-109 mmhg and pill count between 80-100% will enter the randomized phase, counseled regarding salt intake, and randomized double blindly into one of our two treatment arms. we will recruit approximately 105 individuals to have 45 individuals in each drug group for analyses. this assumes that we will have 10-15% non-completers. inclusion criteria: 1. striatin snp rs2540923 allele carrier or both striatin snp rs888083 and striatin snp rs6744560 risk allele carrier 2. ages \>17 years; 3. hypertension as defined by primary physician; 4. not on more than two anti-hypertensives; 5. normal renal, metabolic, electrolyte, complete blood cell count, and lipid profile laboratory tests; 6. if on an angiotensin converting enzyme inhibitor, angiotensin receptor blocker or mineralocorticoid receptor antagonist, needs to be washed out for 3 months. exclusion criteria: 1. known cardiac disease other than hypertension 2. renal, circulatory or neurologic diseases 3. diabetes; smoking 4. secondary hypertension as indicated by history, physical examination or screening blood and urine tests; any drug therapy, except for anti-hypertensives and replacement thyroid medication.