first-time-in-human (ftih) study to evaluate safety, tolerability, pharmacokinetics and pharmacodynamics of single (in both fed and fasted states) or repeat doses of gsk3358699

Conditions: Arthritis, Rheumatoid

Summary: this ftih study, intends to identify the doses of gsk3358699, which are well tolerated by the subjects whilst delivering a robust pharmacodynamic (pd) response. this study will evaluate the safety, tolerability, pharmacokinetic (pk) and pd profile of single (in both fed and fasted states) and multiple ascending doses of gsk3358699 in healthy male subjects within a pre-defined and controlled pharmacodynamic and pharmacokinetic range for each cohort. it also intends to understand the effect of gsk3358699 on systemic markers of inflammation following low dose in vivo lipopolysaccharide (lps) or granulocyte-macrophage colony-stimulating factor (gm-csf) challenge and local inflammation in cantharidin-induced blisters. the study has been carefully designed to explore the in vivo biology of the target and the potential for the study drug to become a transformative medicine for subjects in multiple immuno-inflammatory disease indications.

subjects who are enrolled in the dose escalation treatment periods of part a may choose to only take part in the dose escalation treatment periods 1-3, or may choose to also take part in the challenge treatment period (period 4). if a subject chooses to participate in the dose escalation treatment periods 1-3 only, or does not (at screening) meet the eligibility criteria specific to challenges (treatment period 4), a new subject will be recruited for treatment period 4 only and will be regarded as a replacement subject. the study will be conducted in three parts. total duration for participation will be approximately 19 weeks for subjects taking part in all three dose escalation treatment periods and 23 weeks if a subject takes part in all four treatment periods of part a. for replacement subjects only taking part in the challenge treatment period (period 4), approximate study duration will be 10 weeks. total duration for participation will be approximately 9 weeks for part b and 12 weeks for part c. the study will be conducted in up to 80 subjects.

Eligibility criteria:
inclusion criteria: - subjects enrolled into the study, where they will be administered lps or gm-csf challenge, must be 18 to 55 years of age inclusive, at the time of signing the informed consent. subjects enrolled into the study where they will not be administered lps or gm-csf challenge must be 18 to 65 years of age inclusive, at the time of signing the informed consent. - subjects must be overtly healthy as determined by medical evaluation including medical history, physical examination, laboratory tests and cardiac monitoring. - body weight must be \> = 50 kilogram (kg) and body mass index (bmi) within the range 18.5-35.0 kg per square meter (kg/m\^2) (inclusive). - male subjects agreeing to use contraceptive methods during the treatment period and for at least 91 days, after the last dose of study treatment and refrain from donating sperm during this period. - capable of giving informed consent. exclusion criteria: - current or chronic history of pancreatitis, diabetes mellitus or impaired glucose tolerance, gastrointestinal disease, liver disease, or known hepatic or biliary abnormalities (with the exception of gilbert's syndrome or asymptomatic gallstones), anaphylaxis, and /or anaphylactoid (resembling anaphylaxis) reactions \[sampson et al 2006\], cardiac disease including clinically significant ventricular arrhythmias or long qt syndrome, renal disease where clinically significant (minor abnormalities may be permitted base on discussion between investigator and medical monitor), respiratory disease or conditions including but not limited to asthma, chronic obstructive pulmonary disease (copd), and bronchiectasis and any current respiratory infection (childhood asthma is not an exclusion criterion), sensitivity or severe allergic responses to any of the challenge agents or cantharidin, or components thereof or a history of drug or other allergy that, in the opinion of the investigator or glaxosmithkline (gsk) medical monitor, contraindicates their participation; frequent vasovagal syncope, surgery requiring general anaesthetic or significant trauma in 3 months leading to study enrolment, relevant skin conditions (e.g. recent history of eczema or recurrent eczema, keloid, skin allergies, psoriasis, atopic dermatitis, and vitiligo) which in the opinion of the investigator could pose safety issues or cause interference with study procedures, sepsis, coagulation disorders, peripheral edema, lymphangitis, lymphedema, pleural or pericardial effusion, hemorrhage (eg sub-arachnoid) or hemophilia or a related bleeding disorder. - history of malignancies e.g. recurrent basal cell carcinoma, hematological malignancy. - for subjects receiving cantharidin: presence on either forearm of tattoos, naevi, hypertrophic scars, keloids, hyper- or hypo- pigmentation that may, in the opinion of the investigator, interfere with study assessments. subjects with very fair skin, very dark skin, excessive hair or any skin abnormalities that may, in the opinion of the investigator, interfere with study assessments. - family history of premature cardiovascular disease or long qt syndrome. - qt interval with fridericia's correction (qtcf) \> 450 millisecond (msec), based on averaged qtcf values of triplicate ecgs obtained over a brief recording period. - unable or unwilling to refrain from taking prescription or non-prescription drugs (including vitamins and dietary or herbal supplements) within 7 days (or 14 days if the drug is a potential enzyme inducer) or 5 half-lives (whichever is longer) before the start of study treatment until completion of the follow-up visit. paracetamol, at a dose of \ 1.5x upper limit of normal (uln) at screening. - bilirubin \>1.5xuln (isolated bilirubin \>1.5xuln is acceptable if bilirubin is fractionated and direct bilirubin \ 3.4 millimole per liter (mmol/l) at screening. - fasted total cholesterol \>7.7 mmol/l at screening. - random glucose \> = 11.1 mmol/l at screening. - urinary cotinine levels indicative of smoking or history or regular use of tobacco- or nicotine-containing products within 3 months prior to screening. - history of regular alcohol consumption within 6 months of the study defined as: an average weekly intake of \>14 units. one unit is equivalent to 8 gram of alcohol: a half-pint (approximately 240 milliliter \[ml\]) of beer, 1 glass (125 ml) of wine or 1 (25 ml). - where participation in the study would result in donation of blood or blood products in excess of 500 ml within a 56 day period. - unable to comply with precautions to minimize phototoxicity risk.