8-hydroxy-2-deoxyguanosine ,total antioxidant capacity in oral premalignant and malignant lesions

Conditions: Premalignant Lesion

Summary: objectives: investigating the level of salivary 8-hydroxy-2-deoxyguanosine (8-ohdg) and total antioxidant capacity (tac) in oral premalignant and malignant lesions in order to determine their diagnostic value for the malignant patients. design: 120 subjects diagnosed with oral leukoplakia, oral lichen planus (olp) and oral squamous cell carcinoma (oscc) along with healthy control subjects were included. salivary samples from all participants were collected. 8-ohdg was measured by elisa technique and the tac level was assessed by a photometric test system.

detection of molecular markers in saliva is superior to their detection in serum and other body fluids due to the fact of being an easy non-invasive tool requiring no training for its collection. consequently, the aim of our work was to investigate salivary 8-ohdg and tac in premalignant and malignant patients compared to normal subjects and to determine their diagnostic value for the malignant patients. methodology: study population: a total of 120 subjects were enrolled in the present study. the clinically tested and confirmed subjects included group a: 30 patients diagnosed with oral leukoplakia group b: 30 patients suffering from oral lichen planus group c: 30 patients having oral squamous cell carcinoma with the tongue being the most commonly affected site group d: 30 age and gender matched individuals having no oral mucosal lesions acting as a control group. comprehensive oral diagnosis was done for all participating individuals. biopsy specimens were obtained from various lesions where a surgical double wedge incisional biopsy was carried out to a depth of about 2mm. specimens were sent for histopathological examination to confirm the clinical diagnosis. salivary sample collection: collection of whole unstimulated saliva from all participants was performed using standard techniques.subjects refrained from eating, drinking, chewing gum etc., for at least ½ h before the evaluation.samples were collected by asking individuals to swallow first, tilt their head forward and expectorate all saliva in a tube for 5 minutes without swallowing. after collection, all samples were immediately stored at -20ºc until assayed. detection of salivary 8-ohdg: saliva was centrifuged for 5 minutes at 5000 x g. the supernatant was removed for determination of 8- hydroxy-2'-deoxyguanosine using an elisa kit provided by biovision, usa (catalog # k4160-100). 8-hydroxy-2'-deoxyguanosine is an oxidized derivative of deoxyguanosine and is one of the major products of dna oxidation. determination of salivary total antioxidative status/capacity (tas/tac) was done using imanox (tas/tac) kit provided by immun diagnostik, germany. it is a photometric test system used for the determination of the total antioxidative status/capacity in edta-plasma, serum and other biological samples. the determination of the antioxidative capacity was performed by the reaction of antioxidants in the sample with a defined amount of exogenously provided hydrogen peroxide (h2o2). the antioxidants in the sample eliminate a certain amount of the provided h2o2. the residual h2o2 was determined photometrically by an enzymatic reaction which involves the conversion of tmb to a colored product. after addition of a stop solution, the samples were measured at 450 nm in a microtiter plate reader. the quantification was performed by the delivered calibrator. the difference between applied and measured peroxide concentration in a defined time period is proportional to the reactivity of the antioxidants of the sample (antioxidative capacity). quantification was performed with the enclosed calibrator.

Eligibility criteria:
inclusion criteria: all included individuals were not under any current medication, suffering from any systemic condition or having any other oral mucosal disorder. exclusion criteria: 1. pregnant or lactating females 2. subjects having systemic disorder or taking medications; 3. subjects who suffer from any other mucosal lesions. 4. severe periodontal inflammation

Keywords: squamous cell carcinoma 8-hydroxy-2- deoxyguanosine antioxidant