using a contact dermatitis model with biologic medications to study skin inflammation

Conditions: Skin Inflammation; Allergic Contact Dermatitis

Summary: the purpose of this study is to answer: how do inflammation and anti-inflammatory skin therapies work in the skin? inflammation is a protective response from the body's immune system to injury, disease, or irritation. it is a process by which your body's white blood cells and the things they make protect you from infection from outside invaders such as bacteria and viruses.

the purpose of this study is to study mechanisms of human skin inflammation by using an established model of transient contact dermatitis with pre-treatment by biologic drugs that block specific inflammatory signals or by topical steroids that block broad inflammatory signals. contact dermatitis will be induced in a safe and controlled manner through the use of topical application of squaric acid dibutyl ester (sadbe), along with other common allergens, after which skin will be sampled for analysis using nonscarring skin biopsy techniques including suction blister biopsies and/or application of absorptive microneedle patches. this irb protocol will use select fda-approved, commercially available biologic drugs and topical steroids that have good safety profiles and block inflammatory signals that we observed in our previously acquired data of contact dermatitis. this study will provide insight into human immunology that will deepen our understanding of dermatologic disease, as well as increase our understanding of topical steroids and biologic treatments which sometimes cause paradoxical inflammation despite being designed to suppress inflammation. we hope this will improve the basic understanding of human skin inflammation in order to ultimately impact treatment strategies for several skin diseases.

Eligibility criteria:
inclusion criteria: * healthy adult subjects over the age of 18 years with no skin diseases * patients with dermatologic conditions such as atopic dermatitis, history of localized non-melanoma, keratinocytic skin cancer * patients with previous clinical patch testing * umass medical school students and employees are eligible to participate. * non-english-speaking individuals are also eligible with the assistance of an interpreter and an approved short form consent in the appropriate language. exclusion criteria: * adults unable to give consent * history of the following specific dermatologic conditions (which would be confounders due to their particular immunologic etiologies, specifically the tnfa and il-17 pathways which oppose the th2 pathway): pityriasis rubra pilaris and psoriasis * patients actively receiving whole body phototherapy * patients actively receiving systemic broad-spectrum immunosuppression (prednisone, mycophenolate mofetil, azathioprine, methotrexate) * any history of poor wound healing * history of uncontrolled diabetes * history of easily torn skin * any known cardiac arrhythmia or history of heart failure * history of demyelinating disease * history of liver disease or alcohol abuse * history of melanoma * pregnant women * individuals who are high risk for tuberculosis including prisoners, immigrants from tb- endemic areas, or us-based travelers who have visited tb-endemic areas * individuals with a self-reported personal history of infection with latent or active tuberculosis, hiv, hepatitis b, or hepatitis c will not be included, because the type of immunotherapies that will be used in this study may interfere with these conditions. * for similar reasons, we will not be including individuals with signs of current or active infection, self-reported personal history of recurrent infections, or conditions that compromise the immune system, such as patients with malignancy (except non- melanoma, keratinocytic skin cancers).

Keywords: skin inflammation allergic contact dermatitis