oncological prognosis of prostate lesions by chemical exchange saturation transfer magnetic resonance imaging (cest-mri)

Conditions: Suspected Prostate Cancer (increased PSA Level)

Summary: prostate cancer is the second most common type of cancer in men only after lung cancer, and accounts for \~3.8% of all deaths caused in men in 2018. multiparametric mri, which combines anatomical mri sequences (t2-weighted acquisitions) and functional sequences (dwi diffusion-weighted imaging or dynamic contrast-enhanced (dce) acquisitions), constitutes a key tool in the detection and monitoring of the therapeutic response in the context of the management of this prostate cancer. in this context, a recognized radiological consensus has been established in order to standardize mri acquisition protocols and the data processing scheme (prostate imaging reporting and data system, pi-rads protocol). although the improvement provided by multiparametric mri is significant for the detection of prostate cancer in the clinic, approximately one third of cases remain equivocal. there is therefore a clear clinical advantage in having additional information to support the diagnosis by discriminating between low-grade, intermediate-grade and high-grade tumors and thus reduce the need for biopsies. cest (chemical exchange saturation transfer) mri is a promising molecular imaging tool for specifically detecting metabolites containing exchangeable protons present on amide, amine or even hydroxyl functions. mri-cest allows non-invasively to highlight molecules of interest such as lactate, glutamate, citrate and other mobile proteins and peptides involved in the carcinogenesis process and usually impossible to visualize by mri or mrs (magnetic resonance spectroscopy). exchangeable protons also have the property of seeing their exchange rates modified depending on the surrounding ph, making this cest contrast sensitive to ph variations. then, it is apt-cest (amide proton transfer cest). the first efforts to translate this method from the preclinical to the clinic were made for brain imaging of gliomas with a significant contribution of apt-cest in the stratification of tumors. now, work is expanding to the imaging of other types of tumors such as pelvic tumors, digestive tumors or even breast or lung cancer. currently, only a few studies have explored cest mri in prostate cancer with several limitations both in terms of data acquisition strategy and signal processing. the expertise of the preclinical component of the ivia platform (in vivo imaging auvergne) will make it possible to remove these obstacles and highlight the interest of cest mri for the diagnosis and identification of the tumor stage. thus, the addition of cest-mri to the multiparametric mri strategy will complete the morphological and functional analysis with metabolic criteria that can be decisive in guiding the diagnosis. the main objective of this study is to study the relationship between the apt-cest parameter measured in cest mri and the gold standard given by the gleason score (from the anatomopathological analysis of prostate biopsies), these two data being established for each patient. secondary objectives are: * to study the association between all the cest mri parameters (apt-cest, and magnetization transfer ratio with asymmetric analysis (mtrasym) measurements, guanidyl/apt ratio, nuclear overhauser enhancement (noe) signal) and: * the gleason score * the pi-rads score, calculated from the readings taken for each of the suspicious lesions identified on multiparametric mri (t2-weighted anatomical acquisitions and diffusion-weighted dwi or dynamic dce acquisitions); * the measurement of the blood prostate-specific antigen (psa) level; * the volume of the prostate, determined on the anatomical mri images. * to situate the predictive potential of cest measurements in relation to other variables a priori associated with the gleason score.

this prospective, single-center study will make it possible to evaluate the capabilities of cest mri to predict oncological prognosis by comparing it to the reference multiparametric mri examination and to the anatomopathological analysis of samples taken from biopsies. when the patient presents a suspicion of prostate cancer identified by an increase in the blood concentration of prostate specific antigen (psa), he is usually seen in consultation by the urologist within the urology department of the university hospital. it is during this consultation, after having informed the patient of the need to carry out an mri to rule out or confirm the presence of a lesion, that the doctor will propose the study to the patient, inform him, and grant him a period of reflection, will answer all his questions and will jointly sign the consent with him if he wishes to participate. the imaging examination will be carried out a maximum of 6 weeks after the inclusion visit in the research slots of the 3t mri at chu gabriel montpied (wednesdays and fridays). patients will follow the usual imaging protocol with first multiparametric mri acquisitions including sequences with injection of contrast product (gadolinium) then a cest mri sequence whose duration will not exceed 15 min. as part of the patient's classic course, if the presence of one or more lesions is proven, a sample of the lesion(s) is taken for an anatomopathological analysis which will give the degree of tumor involvement. the patient returns to the university hospital approximately 4 weeks later for a consultation for the anatomopathological results and the proposal for subsequent management (monitoring or treatment). the following data will be noted: age, medical history, psa level in the blood (nanogram / millilitre), pi-rads score for each suspicious lesion identified on multiparametric mri acquisitions, extension assessment, parameters measured from the cest mri sequence for each of the suspicious lesions: atp-cest rate (in arbitrary units), mtrasym, guanidyl/apt ratio making it possible to assess tumor acidosis, pathology of samples from the lesion(s): presence or absence of lesions or cancer cells, their number, extent and nature using the gleason score. the main objective of this study aims to study the predictive properties of a cest mri in the tumor classification of prostate tumors, considering the presence or absence of lesions or cancer cells, their extent and their nature through the score of gleason on pathological examination of biopsy specimens as dependent variable of interest and measurements on apt-cest images as main explanatory variable. false negative rates, diagnostic sensitivity and specificity values and negative and positive predictive values will be presented with a 95% confidence interval. the concordance between anatomopathological examination and cest mri will be studied by estimating the rate of concordant cases and kappa concordance coefficient. the usual inferential statistical tests will be considered: for comparisons concerning variables of a quantitative nature: student's t test or mann-whitney test if the conditions for applying the t-test are not respected; for categorical parameters: chi2 test or fisher exact test. particular attention will be paid in particular to the time between the mri and the pathological examination (3 weeks on average).

Eligibility criteria:
inclusion criteria: patients will be included: * aged over 18 * with suspected prostate cancer identified by an increase in blood psa concentration * naïve to chemotherapy, radiotherapy or hormonal therapy * affiliated to a social security system * able to give informed consent to participate in research * understanding and reading french well exclusion criteria: patients will be excluded: * refusing to participate in the study * with impaired understanding, under guardianship/curatorship/deprived of liberty * presenting "contraindications" to an mri examination (with administration of a gadolinium chelate): presence of metal parts in the body (electronic devices such as a cardiac pacemaker, a neurostimulator, a cochlear implant, prostheses, intra foreign bodies ocular...), claustrophobia, obesity * at risk: 1. . history of allergy to gadolinium (contrast product necessary for performing the multiparmatric mri planned in the routine care pathway 2. . history of hypersensitivity to gadoteric acid or gadoline contrast agents, meglumine * during a period of exclusion from another study

Keywords: suspected prostate cancer increased psa level cest-mri guanidyl/apt ratio to assess tumor acidosis, tumor classification gleason score pi-rads score