interaction between immune cells and bacteria associated with periodontitis

Conditions: Periodontal Diseases; Periodontitis; Aggressive Periodontitis; Immunologic Disease; Microbial Disease; Periodontal Pocket; Inflammation; Inflammation Gum; Dysbiosis; Rheumatoid Arthritis; Generalized Aggressive Periodontitis; Generalized Chronic Periodontitis; Chronic Periodontitis

Summary: this study evaluates the interaction between host immune cells and bacteria associated with periodontitis. it comprises biological material from donors with and without periodontal disease. specifically, we collect a spit and blood sample to conduct in vitro stimulations and measurements of selected parameters related to periodontitis to clarify obscure areas in the immunologic pathogenesis of this disease.

periodontitis is a prevalent, multifactorial inflammatory disease characterized by the interaction between microorganisms organized in biofilms on tooth surfaces and host immune cells, leading to an inflammatory destruction of the tooth-supporting tissues and - if left untreated - eventually tooth loss. periodontitis affects up to 50% of the population in the united states of america, and is classified in an aggressive and a chronic form depending on genetic factors, age of onset, speed and severity of attachment loss. the onset of periodontitis is caused by an immunologic imbalance between host immune cells and residing microorganisms in subgingival pockets. the host immune cells are capable of enhancing both a protective and a destructive inflammatory response towards the microorganisms through the release of inflammatory mediators e.i. proinflammatory and antiinflammatory cytokines. the role of antibodies in periodontitis is also unclear. some studies show an excessive antibody level against bacteria associated with periodontitis e.g. porphyromonas gingivalis (p.g.). in general, this study contributes to a profound understanding of the host immune cells role in the onset and pathogenesis of periodontitis by comparing healthy versus diseased donors immunologic responses toward pathogene and apathogene microorganisms and their genetic background.

Eligibility criteria:
inclusion criteria for chronic periodontitis donors: * 50-60 years of age. * interproximal attachment loss at minimum 3 teeth besides molars and incisors. * clinical attachment loss at minimum 10 sites identified by bleeding and pus upon probing. * visible radiographic bone loss. * medically healthy donors. inclusion criteria for aggressive periodontitis donors: * 19-40 years of age. * interproximal attachment loss at minimum 3 teeth besides molars and incisors. * clinical attachment loss at minimum 10 sites identified by bleeding and pus upon probing. * visible radiographic bone loss. * medically healthy donors. inclusion criteria for healthy donors: (age: 19-40 years; 50-60 years) * no sign of inflammatory conditions or other general systemic diseases. * medically healthy donors. exclusion criteria for all groups: * pregnant and breastfeeding. * antibiotic treatment within 6 months. * suffer from periodontal manifestations caused by systemic diseases e.i. genetic diseases, haematologic anomalies or syndromes.

Keywords: periodontal disease periodontitis aggressive periodontitis dysbiosis inflammation regulation cytokines bacteria porphyromonas gingivalis saliva anti-ccp peptidyl arginine deiminases single nucleotide polymorphisms