the underlying mechanisms for s. aureus infection and colonization of skin in people with atopic dermatitis with and without eczema herpeticum (mrsa)

Conditions: Methicillin-Resistant Staphylococcus Aureus; Atopic Dermatitis; Eczema Herpeticum; MRSA

Summary: staphylococcus aureus (s.aureus) is a bacterium that causes many painful skin and soft tissue conditions, such as scalded-skin syndrome, boils, or impetigo. serious cases may result in deadly complications but s.aureus can usually be treated successfully with antibiotics. there are, however, certain strains which cannot be treated with standard antibiotics. methicillin-resistant staphylococcus aureus (mrsa) is one such strain. mrsa is increasingly being seen in both hospital and community settings, making it a serious public health issue. people with atopic dermatitis (ad), particularly those with a history of eczema herpeticum (eh), may be at greater risk for infection by mrsa. the reason for this higher risk is unknown but may be linked to extended treatment with staphylococcus antibiotics in addition to the absence of certain proteins on their skin, which have immune function. the purpose of this study is to determine the reasons for mrsa infection in ad participants with and without a history of eh.

the emergence of drug-resistant staphylococcal strains was first seen in the us almost a decade ago, when the centers for disease control (cdc) reported four fatal community-based mrsa infections in children. in recent years, mrsa has grown into a serious public health concern with an increasing number of both hospital-acquired and community-acquired cases being seen. there will be approximately 130 participants recruited for this trial. the study population will consist of people with atopic dermatitis (ad) and people without atopic dermatitis (non-atopic). ad is a skin disorder with an itchy, red skin rash. people with ad are more likely to get bacterial and viral skin infections, possibly because they lack certain proteins in their skin, which help the body's immune system to fight infections. ad people with a history of eczema herpeticum (eh) may also be at greater risk for being infected with mrsa. this could be due to extended treatment courses with staphylococcal antibiotics, especially because overuse and misuse of antibiotics can lead to bacterial antibiotic resistance. the precise reasons are unknown. the purpose of this study is to determine the reasons for infection in ad participants with and without a history of eh. investigators are seeking to recruit patients with either methicillin-sensitive staphylococcus aureus (mssa) or mrsa bacteria on their skin, so that they may adequately study potential factors related to mrsa infection. investigators will determine if the mrsa collected from people with ad is primarily community or hospital associated. they will also determine if the proteins on the skin of adeh+ people with mrsa differ from the proteins on the skin of ad people with mssa or people without ad, or if there are any marked differences in serum total ige levels between ad subjects with mrsa, mssa, or without s. aureus. approximately 60 adeh+ and 60 adeh- participants will need to be enrolled to find participants with mrsa or mssa on their skin. presence of these bacteria on the skin can only be determined once skin swabs are collected and tested. if participants are deemed eligible at screening, they will continue on to the study visit, which will last for approximately 2-3 hours. at the study visit, participants will be asked to provide information related to their medical history including infection, hospitalization, and medication record. additionally, a skin exam will be performed to verify diagnosis (adeh-, adeh+, or non-atopic); nasal and skin swabs samples will be collected; tape strippings samples will be collected; and a blood sample will be collected.

Eligibility criteria:
inclusion criteria: * parent, or legal guardian willing to provide informed consent, if necessary * residing in the u.s. * have active ad with or without a history of eh as diagnosed using the advn standardized diagnostic criteria or are non-atopic as diagnosed using the advn standardized diagnostic criteria exclusion criteria: * history of any systemic illness (i.e., immunodeficiency disorders such as human immunodeficiency virus \[hiv\] or lupus erythematosus) other than the condition being studied * presence of active systemic malignancy, excluding uncomplicated non-melanoma skin cancer * presence of any skin disease other than ad that might compromise the stratum corneum barrier (e.g., bullous disease, psoriasis, cutaneous t cell lymphoma \[also called mycosis fungoides or sezary syndrome\]) * use of topical medications including (but not limited to) elidel, protopic, or topical corticosteroids at the site of the skin lesion within the last 3 days * use of topical antibiotics within the last 24 hours * use of oral antibiotics within the last 10 days. subjects who are known to be culture positive for mssa or mrsa despite antibiotic treatment will not be excluded. * history of serious or life-threatening reactions to tape or adhesives will be excluded from the tape stripping procedure

Keywords: methicillin-resistant staphylococcus aureus mrsa methicillin-sensitive staphylococcus aureus mssa atopic dermatitis eczema herpeticum