Inclusion Criteria:  Fit and healthy subjects aged < 18-year-old; Subjects with maxillary permanent incisor subjected to a traumatic injury without any dentoalveolar trauma involvement and have a contralateral homologous tooth with no signs/symptoms of injury; Subjects attended their first dental visit within 3 days of the dental trauma; Sensitivity value of the electric pulp test over 200 μA; Subjects with at least one vital and non-traumatised maxillary incisor acting as a control tooth; Ability to obtain a verbal acceptance from all the subjects to comply with all the treatments and follow-up timepoints attendance; Ability to obtain written informed consent by the patients' parents/guardians for treatment, participation in the study and publication  Exclusion Criteria:  Subjects who were undergoing therapy for neurological conditions or with mental or cognitive problems; Subjects who were taking sedative, analgesic, and/or anti-inflammatory medication 7 days prior to the treatment commencement; Subjects who have never had any first dental visit or treatment; Subjects with systemic diseases or physiological development delay; Subjects with active infectious diseases such as; influenza, scarlet fever, etc. Subjects with restorations covered more than half the labial surface of the investigated teeth.