Inclusion Criteria:  Patients who have no major organ dysfunction. Diagnosed as moderate to severe knee osteoarthritis according to internationally recognized criteria: a). Meets the criteria of the "Guidelines for the Diagnosis and Treatment of Osteoarthritis (2018 Edition)" revised by the Chinese Medical Association Rheumatology Branch in 2018; b). Meets the criteria of Grade 3 or more according to the American College of Rheumatology (ACR); c).Meets the criteria of Grade Ⅲ or more according to Kellgren-Lawrence's OA radiology grading standard. Those who have the ability to move independently, except those who use wheelchairs, walkers or crutches, and who have no history of mental illness. There is no obvious contraindication to articular cavity injection in hematology and biochemical testing. No local or systemic infection. Subjects and their families understand the clinical trial protocol and agree to participate in the trial, and sign a written Informed Consent. Patients whose pain has lasted for more than half a year, and the routine clinical treatment of oral hormones, opioids and other drugs have been ineffective.  Exclusion Criteria:  Those who are older than 70 years old or younger than 18 years old, or have no full capacity for civil conduct. HIV, hepatitis virus or syphilis virus infection or positive serological test. Body mass index (BMI) greater than 30 kg/m2. Congenital or acquired knee deformity; severe knee arthritis accompanied by severe varus deformity, with varus greater than 10°. Pregnant or lactating women, or women of childbearing age who have a positive pregnancy test within 7 days before receiving treatment. Complicated with severe cardiovascular and cerebrovascular, liver, kidney, and endocrine, blood system diseases, malignant tumors, allergies, mental disorders, acute infections or local knee joint infections. Patients with immunodeficiency. Patients with a history of intra-articular injection within 3 months; patients being treated with immunosuppressive agents and glucocorticoids. Patients who are still participating in other clinical trials. Subjects who refuse to sign Informed Consent or refuse to participate in this clinical trial.