Inclusion Criteria:  Inclusion criteria of liver and kidney deficiency syndrome and phlegm and blood stasis syndrome in knee osteoarthritis  Age 20-80 years old, in line with the diagnostic criteria of Western medicine; In accordance with the diagnostic criteria of TCM syndromes, it belongs to liver and kidney deficiency syndrome and phlegm and blood stasis syndrome; Patients who are willing to participate in the survey and have good compliance; Voluntary signing of informed consent;  Inclusion criteria of normal control group  Age 20-80 years old. Patients who are willing to participate in the survey and have good compliance. It does not meet the diagnostic criteria of knee osteoarthritis. Voluntarily sign informed consent.  Exclusion Criteria:  Complications affecting joints, such as psoriasis, syphilitic neuropathy, brown yellow disease, metabolic bone disease, etc; There were rheumatoid arthritis and ankylosing spondylitis; Late disabled and incapacitated; Pregnant or lactating women; Mentally ill. As long as one of the above 5 articles is met, it shall be excluded.