Inclusion Criteria:  The normal & high responders were selected according to :  age: 18 - 40 years anti-Müllerian hormone (AMH): > 1.0 ng/ml number of aspirated oocytes:more than or equal 8 BMI < 34  Exclusion Criteria:  Age > 40 years; AMH < 1.0 ng/mL; aspirated oocytes < 8. severe male factor Testicular aspirated sperm