Inclusion Criteria:  Ability to understand and willingness to sign an informed consent form Elective lumbar spinal surgery that will take at least 120 minutes, American Society of Anesthesiologists (ASA) physical status I and II Be between the ages of 18 and 65  Exclusion Criteria:  Patients with ASA physical status III or IV disease Smoking or alcohol consumption Chronic systemic disease (diabetes mellitus,chronic pulmonary disorders, chronic kidney and liver disorders recently received radiation, chemotherapy malignancy blood transfusion in operation use of antioxidant supplements have received general anesthesia in the last three months Patients with known occupational exposures (operating room personnel, chemical plant workers