Inclusion Criteria:  Preterm and term infants admitted to the NICU for the first time for various reasons with a hospital stay ranging from 1 to 8 days Preterm and term infants who were cared for in an incubator without any pharmacological or non-pharmacological analgesic therapy, muscle relaxant, and sedation treatment in the last 24 hours before the procedure.  Exclusion Criteria:  Exposed to painful procedures more than 3 times in the same day Infants who diagnosed with osteomyelitis, sepsis, pyejonic arthritis, congenital anomaly (Spina bifida, arthrogryposis multiplex congenita) Infants with any known neurological diagnosis (Abnormal MRI finding, hydrocephalus, Chiari malformation, asphyxia, periventricular leukomolacia (PVL), acute bilirubin encephalopathy, hypoxic ischemic encephalopathy (HIE)) Infants who had any surgery