Inclusion Criteria:  Diagnosed and hospitalised patients with mild acute cholecystitis  Exclusion Criteria:  Pregnant women Immunosuppressive patients Patients with intermediate and severe acute cholecystitis Patients with acalculous acute cholecystitis Patients with uncontrolled diabetes mellitus Patients with collegen tissue diseases Patients with malignancies Patients who are using anti-coagulant or anti-aggregant medications Patients with blood diseases Patients with BMI>35