Inclusion Criteria:  Age 18-75 Capacity to give consent, provide samples, and follow-up at routine clinic appointments  Exclusion Criteria:  Antibiotic use (outside of 72 hour period prior to diagnosis or peri-operative short course) within the 3 months prior to enrollment Unable to provide consent or samples immunocompromised conditions such as HIV/AIDS, SLE, organ or bone marrow transplant recipient, on immunosuppressants for autoimmune disease, genetic disorders such as cystic fibrosis that may result in substantial alteration in colonized community. inability to provide 3mL of saliva without stimulation critical illness