Inclusion Criteria:  Scleroderma individuals according to 2013 American College of Rheumatology/European League against Rheumatism (ACR/EULAR 2013) classification criteria for SSc.  Exclusion Criteria:  insufficient data to enable classification any acute bacterial infection abnormal kidney function comorbidity other chronic disease degenerative disease such as diabetes and malignancies subjects who received biological therapy or mycophenolate-mofetil during the previous month.