Inclusion Criteria:  Systemically healthy (The determination of healthy volunteers will be based on the statements of the patients in the anamnesis. No additional examinations will be made.), Having a total of at least twenty permanent teeth in the mouth, Non-smoker Have not used antibiotics, anti-inflammatory and systemic corticosteroid drugs in the last 6 months, Not in pregnancy or lactation period, Patients who have not received periodontal treatment in the last 6 months, Patients who accepted the research and signed the informed volunteer consent form Bleeding on probing in 30% or more areas, 5 mm or more probing pocket depth in each quadrant in at least 2 non-adjacent teeth 4mm or more attachment loss, Coronal 1/3 or more (horizontal and/or vertical) on radiograph individuals with bone loss were included  Exclusion Criteria:  Having any systemic disease, Regularly using a systemic medication, Being pregnant or lactating. To have had periodontal treatment in the last 6 months. Receiving antibiotic, anti-inflammatory or systemic corticosteroid medication in the last 6 months. Smoking.