Inclusion Criteria:  - Inclusion criteria  Sixty Postmenopausal women (55-65 y) Body mass index (BMI) ranged from 35to 39.9 kg/m2 Postmenopausal more than 2 years  Exclusion Criteria:  Exclusion criteria  women receiving weight-reduction interventions taking lipid lowering drugs regular medications(e.g., β-blockers, α-blockers, digoxin, diuretics, aspirin, nitrates, Presently using sex hormones) having active chronic illness (e.g., rheumatoid arthritis, hyperthyroidism, and inflammatory bowel disease) and diabetes mellitus or other (unstable) endocrine-related diseases cognitive impairment that will make it difficult to partake in the study presence of malignant disease blood donation within the last 30 days Participation as a subject in any type of study or research during the prior 90 days smoking