Inclusion Criteria:  The electrocardiogram showed T wave change and ST segment depression New York Heart Association (NYHA) Classification of cardiac function Ⅰ-Ⅱ level The patient who first time to receive knee replacement General anesthesia lasted about 2h Signed informed consent  Exclusion Criteria:  Does not meet the above selection criteria Unable to sign the informed consent Patients with a malignant tumor, other serious systemic diseases, or psychosis Pregnancy, lactation, and those who are not pregnant but do not take effective contraceptives measures The patient with a history of an allergic reaction to biological products or drug The patient has any infectious diseases (including bacterial and viral infections) The patient with cardiac pacemaker implantation within 3 months prior to enrollment The patient who had a stroke within 6 months prior to enrollment Unable to comply with the agreed timetable of this study Patients who are participating in other clinical trials Others who are clinically considered unsuitable for this treatment.