Inclusion Criteria:  Women aged 20 and over. The result of cervical histopathology in the last 3 months was low-grade squamous intraepithelial lesion (LSIL). Non pregnant people with sexual history. Asexual life, no vaginal medication or flushing before 72 hours of sampling.  Exclusion Criteria:  Within 8 weeks after pregnancy or postpartum. Patients with history of genital tract tumor. History of HPV vaccination. Previous history of hysterectomy, cervical surgery, pelvic radiotherapy Historical. In recent one month, she has received genital tract infection, HPV or other STDs treatment related to the infection of mycoplasma. Use antibiotics or vaginal microecological improvement products in recent 1 month.