Inclusion Criteria:  patients that was diagnosed with non-metastatic breast cancer and anthracycline-based chemotherapy were planned for them  Exclusion Criteria:  Established ischemic heart disease Baseline LVEF <50%, cardiomyopathy (restrictive, dilated or hypertrophic) detected by transthoracic echocardiography Moderate or severe valvular disease, Prior chemotherapy, Presence of contraindication for beta-blockers and cardiac arrhythmias and lack of patient compliance Patients that had consuming angiotensin converting enzyme Inhibitors, angiotensin receptor Inhibitors,diuretics,statins,other beta-blockers or non-dihydropyridin calcium channel blockers