Inclusion Criteria:  Patient age ≥ 18 Female patients must agree to the use of 2 methods of contraception. For man one method of contraception is needed if if his partner is in childbearing age throughout the study and for at least 5 months after last study treatment administration. Patients with metastatic stage III or IV melanoma relapse Eligible for a melanoma metastatic treatment indicated and administered as part of usual care Patients must be willing and able to undergo cutaneous tumor biopsies (except on the face and folds or lymph node) according to the study protocol Patient insured or beneficiary of a health insurance plan Patient able to provide informed consent and sign approved consent forms to participate in the study Patient accepting the conservation of biological samples and their use for clinical research including genetic research  Exclusion Criteria:  Hematologic tumours under treatment Patients with a documented history of autoimmune pathology Ocular melanoma Persons placed under the safeguard of justice Use of immunosuppressants including corticosteroids 4 weeks before the inclusion