Inclusion Criteria:  Aged of 18 years or above Serologically negative for hepatitis B surface antigen (HBsAg), hepatitis B surface antibody (anti-HBs) at enrollment Sign informed consent, willing to participate in this study  Exclusion Criteria:  Being pregnant Intolerance or allergy to any component of the vaccine Participants with severe acute or chronic diseases (such as liver disease, blood disease, cancer), acute onset of chronic diseases and fever