Inclusion Criteria:  High-risk newborns over 32 weeks of age. Those who want to receive blood film collection for newborn screening on the third day of birth. The incubator is used for care during the hospitalization. The mother or father of the research subject can participate in the test all the way. After explaining and reading the consent form, the parents of the research subjects agree to participate in the research and obtain written consent.  Exclusion Criteria:  Apgar Score is still below 7 in the fifth minute. Being treated with a high-frequency positive pressure respirator. Suffering from congenital diseases including: ear canal malformation, cerebral palsy, Down's syndrome, congenital cyanotic heart disease. After the doctor's assessment, the patient should continue fasting due to the condition or restrict breastfeeding on demand. Instability of blood glucose after birth requires close monitoring of blood glucose, and has received more than three (including) heel puncture experiences. Birth injuries of the body due to the birth process, such as broken fetal head and broken collarbone. Are receiving medical treatment with sedatives.