Inclusion Criteria:  Infants born between 22 and 32 gestational age weeks or under 1500 gr birth weight.  Exclusion Criteria:  Infant death before first examination for ROP parents who want to leave from the study Contraindications for breast milk use (Active HIV infection, Active tuberculosis, CMV infection) Inborn error of metabolism like galactosemia. Congenital eye development disorders like agenesis, or situations that do not allow retina examination. other lethal congenital molformations congenital infections that affect the retina like TORCH infections.