Inclusion Criteria:  - Patients who had been recruited into DTCC 1st study will be enrolled.  Exclusion Criteria:  Other malignant tumors;  Severe organ damage such as heart failure of New York Heart Association classes III-IV, liver failure, respiratory failure, renal failure, etc.;  Medical or psychological condition that would not permit the patient to complete the study or sign the informed consent; ④ Legal incapacity or limited legal capacity; ⑤ Unwilling to be followed up