Inclusion Criteria:  All HCC patients in BCLC stage 0-A underwent liver resection in hepatic surgery center, Tongji hospital, Tongji Medical College, Huazhong University of Science and Technology. The inclusion criteria were as follows: 14- 75 years old  Exclusion Criteria:  with any history of malignant tumors; with treatment of ablation, TACE or TKI history before liver resection.