Inclusion Criteria:  Peri-implantitis with:  Presence of peri-implant pocket > 5mm, Bleeding on probing (BOP), Radiographic evidence of bone loss > 3mm, or one or two-third bone loss Implant in function for more than one year (prosthetic rehabilitation for more than six months) Good level of oral hygiene (plaque index <1) No periodontal or peri-implant treatment three months prior to the study No use antibiotics in the last three months No anti-inflammatory drugs were used in the previous two months  Exclusion Criteria:  Buccally implant placement, implant mobility, two-third of bone loss, patient with systemic conditions (diabetes mellitus, leukemia), patient on radiation therapy, drug and alcohol abuse, pregnant and breastfeeding, women