Inclusion Criteria: signed and dated informed consent form, willing to comply with all study procedures and be available for the duration of the study, female, aged 18 to 44, all races and ethnic backgrounds.  Exclusion criteria: current pregnancy or lactation, history of hysterectomy, bilateral oophorectomy, endometrial ablation, uterine artery embolization; and current or previous (within 3 months) hormonal medications or insulin-sensitizers intake, anything that would place the individual at increased risk or preclude the individual's full compliance with or completion of the study; unwillingness to participate or difficulty understanding the consent processes or the study objectives and requirements.