Inclusion Criteria:  Ability to walk, sit down and stand up independently Ability to lie in a supine or slightly elevated position for 8.5 hours BMI between 25 and 35 kg/m2 Willingness and ability to comply with the protocol  Exclusion Criteria:  Established diagnosis of malignancy Established diagnosis of Insulin-Dependent Diabetes Mellitus History of untreated metabolic diseases including hepatic or renal disorder Presence of acute illness or metabolically unstable chronic illness Recent myocardial infarction (less than 1 year) Any other condition according to the PI or nurse that was found during the screening visit, that would interfere with the study or safety of the patient Failure to give informed consent or Investigator's uncertainty about the willingness or ability of the subject to comply with the protocol requirements