Inclusion Criteria:  Healthy BMI <30 systolic blood pressure <140 mmHg diastolic blood pressure <90 mmHg have no chronic disease have no complaints related to the musculoskeletal, respiratory and cardiovascular systems willing to to come to the laboratory willing to sign the informed consent form  Exclusion Criteria:  suffer from chronic disease have complaints related to the musculoskeletal, respiratory, and cardiovascular systems have a history of smoking, both active and passive in the past year