Inclusion Criteria:  Be 18 years of age or older at the time of enrollment Have a body mass index (BMI) of ≤ 35Kg/m2 Be willing and capable of giving written informed consent to participate in this clinical study based on voluntary agreement after thorough explanation of the subject's participation has been provided Be willing and capable of subjective evaluation, reading and understanding written questionnaires, and reading, understanding and signing the written informed consent Has been diagnosed with Mild to Moderate knee osteoarthritis (OA) in one knee only, with a Grade 2 or 3 on the Kellgren Lawrence (KL) grading scale Has an average knee pain intensity ≥ 6 of the Numerical Pain Rating Scale (NPRS); Scale 0 to 10 Be willing to not take any knee symptom modifying drugs from baseline through the End of Study Be willing and able to comply with study-related requirements, procedures, and visits If female, sexually active, and of childbearing age, subject must be willing to use a reliable form of birth control throughout the duration of the study. If Male, sexually active with partners of childbearing age, must be willing to use contraceptive measures  Exclusion Criteria:  Has taken any pain medications, including NSAIDs, within 15 days prior to the study injection date Current use of anticoagulants or history of regular use of anticoagulants History of addiction to dependency producing medications or history of a substance abuse (including alcohol and illicit drugs) Has mechanical knee symptoms consistent with extensive intraarticular pathology not amenable to injection therapy alone, including clinical or imaging evidence indicative of ACL, MCL, LCL, or meniscal pathology Has undergone intraarticular injection of any drug including but not limited to corticosteroids or viscosupplementation in the index knee in the last 3 months History of any type of surgery on the index knee History of traumatic injury to the index knee within the last 3 months Has planned elective knee surgery during the course of the study History of organ or hematologic transplantation History of rheumatoid arthritis or other autoimmune disorders History of immunosuppressive medication/treatment or cancer diagnosis within the last 5 years Current knee infection or history of using antibiotics for knee infection within the last 3 months Has participated in another clinical study or received treatment with any investigational product within 30 days of enrollment Is pregnant as determined by urine testing unless female subject is surgically sterile or post-menopausal Currently breastfeeding or desires to be pregnant during the course of the study Has contraindications to X-ray or MRI imaging Has a diagnosis of progressive neurological disease Has a diagnosis of an active psychological or psychiatric disorder Has pain in other area(s) and/or medical condition(s) that could interfere with accurate pain reporting, study procedures, and/or confound evaluation of the study Has unresolved major issues of secondary gain (e.g., social, financial, or legal (e.g., worker's compensation claim)