Inclusion Criteria:  Male or Female, Age ≥ 18  Willing to comply with protocol instructions, including all study visits and study activities.  Patient with an open Diabetic Foot Ulcer  Adequate arterial blood flow as evidenced by at least one of the following (for wounds below the knee):  TcOM >30 mmHg  Ankle-brachial index ≥0.7-1.20  Toe pressure > 30 mmHg  TBI > 0.6 mmHg  Exclusion Criteria: Individuals who are deemed unable to understand the procedures, risks, and benefits of the study.  Wounds closed or to be surgically closed by flap or graft coverage  Subjects with marked immunodeficiency (HIV/AIDS, or on immunosuppressive medications.  TcOM < 30mmHg  Diabetics with a hemoglobin A1c > 10 within 3 months prior to enrollment  Subject with autoimmune connective tissue disease  Ulcer size and location that does not allow the TEWL measurement per SOP  Pregnant women  Prisoners  Unable to comply with study procedures and/or complete study visits  -