Inclusion Criteria:  gestational age (GA) at birth between 24+0 and 27+6 weeks signed informed consent of parents.  Exclusion Criteria:  One or more of the following:  maternal-fetal immunization hydrops fetalis major congenital malformations associated or not with genetic syndromes previous transfusions hemorrhage at birth congenital infections out-born infants health care team deeming it inappropriate to approach the infant's family for informed consent.