Inclusion Criteria:  All subjects need to sign the informed consent form All subjects have non-small cell lung cancer which suspected on chest CT or confirmed pathologically preoperatively Treatment naïve advanced lung cancer (including extensive stage IIIb and stage IV) Patients who were suitable to provide enough metastatic tumor tissue and blood (30ml) for the single cells sequencing  Exclusion Criteria:  Patients with previous history of lung cancer Have been undergone chemotherapy or radiotherapy due to non-small call lung cancer Have been diagnosed any type of cancer other than cancer of skin, thyroid, uterine cervix within 5 years before enrollment