Inclusion Criteria:  Woman Age 30-50 years old WSRS scale 3 to 4 Willing to follow the research to completion Subjects who do not have a history of filler injection and/implants, laser therapy, microdermabrasion, peeling, botulinum toxin therapy, manual acupuncture on the face, thread embedding acupuncture on the face and does not either oral or topically use collagen within 6 months Body mass index of ≥ 18.5 Signed the informed consent  Exclusion Criteria:  Subjects with facial muscle paralysis disorders Subjects with history of keloids and/or hypertrophic scars, subjects with tumors or infection or inflammation at the treatment area Subjects with allergies to stainless steel, PDO threads, topical anesthetics lidocaine Pregnant and lactating subjects Subjects with cancer Subjects with history of bleeding disorders or are taking anticoagulant or antiplatelet. Subjects suffering from fever (≥ 37.5°C), cough, fatigue Subjects with blood glucose POCT (Point of Care Testing) levels of ≥ 200 mg/dl