Inclusion Criteria:  18+ years old Ambulatory Willing and able to comply with the study protocol  Exclusion Criteria:  Participants with any condition that may preclude venipuncture or venous blood draws will be excluded. Participants with known allergies to interventions will be removed from the study. Vulnerable subjects including children, employees and those with cognitive disabilities will not be included in this study. Male participants on iron supplementation Participants on manganese, biotin, vitamin D and niacin supplementation Pregnant women Nursing women Membership in the clinical study team Any condition that might interfere with volunteer safety, study objectives or the ability of the participant to understand or comply with the study protocol  At the time of enrollment:  Active systemic or serious concurrent illness History of immunodeficiency Any known or suspected impairment of immunologic function Diabetes mellitus treated with any diabetic medication Moderate to severe renal disease Blood pressure greater than 140/90 Chronic hepatitis B or C Recent or current use of immunosuppressive medication Malignancy other than squamous cell or basal cell skin cancer, including solid tumors such as breast cancer or prostate cancer with recurrence in the past year, and any hematologic cancer such as leukemia, which might jeopardize volunteer safety or compliance with the protocol Autoimmune disease Auto-inflammatory disease History of blood dycrasias or hemoglobinopathies requiring regular medical follow up or hospitalization during the preceding year Use of any anticoagulation medication A medical or psychiatric condition or occupational responsibilities that would preclude subject compliance with the protocol History of Guillain-Barre syndrome