Inclusion Criteria:  Age from 18 to 70 years old, no gender limit The primary tumor is located in the right colon, including the ileocecal area, ascending colon, liver flexure, and the right part of the transverse colon. Pathologically proved primary intestinal tumor with simultaneous / metachronous metastasis Molecular pathology confirmed as RAS/RAF wild type Can tolerate and cooperate with the completion of chemotherapy and targeted therapy Tissues can be obtained through puncture or colonoscopy for organoid culture Complete clinical data, as well as efficacy evaluation data, can be obtained  Exclusion Criteria:  Less than 18 years of age or more than 70 years of age The primary tumor is located in the left colon or rectum Pathology results can't confirm as colon cancer RAS and RAF are mutant confirmed by molecular pathology Refuse to cooperate and complete the treatment, or there is a contraindication for chemotherapy or targeted therapy No available metastatic lesions tissue Unable to obtain complete clinical data or efficacy evaluation data