Inclusion Criteria:  Age 45 to 85 years of male or female. Meet the ACR diagnostic criteria, the target knee is confirmed with OA by radiographic images and Kellgren &Lawrence (K-L) score of 2 to 3； Patients who still suffer from OA pain after receiving traditional non-drug therapy or general analgesic therapy within 6 months before the start of the study； The VAS pain score of target knee must be at least 3 cm within 1 week before the screening and if the other side of knee has OA at the same time, the VAS pain score should be less than 3 cm； Subject who has ability to understand the study purpose, comply with the study requirements, willing to stop all pain medications and physical therapy to the knees during the study and sign informed consent form (ICF).  Exclusion Criteria:  The hip or ankle joints are diagnosed with OA, pain, or deformity； K-L score of 4 by radiographic image evaluation； The target knee has symptoms such as infection, redness, and swelling at the screening period and before treatment ； The target knee has received hyaluronic acid treatment within 6 months before included in the study； The target side has received lower limb surgery or knee operation with accompanied complications and significant mobility impairment within 6 months before the start of the study； The target knee has received treatments such as steroid injection, arthrocentesis or arthroscopic surgery within 3 months before screening； Patient with autoimmune disease (such as autoimmune collagenopathy, rheumatoid arthritis), malignant tumors, coagulation disorder, heart diseases, mental diseases…etc. which may cause higher risk to patients during participation in the study； Receiving orthopedic-related treatments which may affect the evaluation of the study； With history of hypersensitivity or allergy to hyaluronic acid or any component of the device; With history of hypersensitivity or allergy to Gram-positive bacteria or Streptococcus proteins； Pregnant, planning pregnancy or in breastfeeding females during the study period； Subject who cannot cooperate with the follow- ups； Other circumstances which judged to be unsuitable for participating in the study by the investigator.