Inclusion Criteria:  Age: 20~80 years Gender: All Poor wound healing after standard wound management for 4 weeks Deep vein thrombosis (DVT) has been ruled out by Duplex and Computer tomography or DVT has been treated Wound care with nursing specialty Recognize and agree to join this trial  Exclusion Criteria:  Autoimmune status, such as systemic lupus erythematosus Heart, lung, kidney or liver failure patients Radiation related wound or cancer wound Those who cannot understand the trial Age less than 20 years or elder than 80 years Pregnancy Those who was Risky to receive general anesthesia or surgery Allergy to Patent Blue V or Gentian Violet