Inclusion Criteria:  patients that required periodontal surgery; patients age between 30-60 years; patients with full mouth plaque score and full mouth bleeding score < 15%; patients with a good general healthy status; patients without any medicaments or drug consumption that can affect the healing process; non-smoking patients.  Exclusion Criteria:  patients in pregnancy; patients in lactation period; patients with consumption of antibiotics or anti-inflammatory drugs in the previous six months; patients with systemic diseases.