Inclusion Criteria:  The subject is a male and aged 20-40. The subject has a usual exercise habit (exercising 2 times or more per week) and did not participate in professional sports competitions or professional sports training during the study period. No smoking or drinking habits. No food allergies and the liver function is normal. No cardiovascular diseases or other chronic diseases, and no serious illnesses requiring hospitalization during the study period. The subject can understand the test process described in the consent form and the possible potential risks and benefits, and can sign the consent form. The subject can accept dietary control during the trial period.  Exclusion Criteria:  Those who have taken nutritional supplements containing branched-chain amino acids. Those who cannot complete the endurance exercise ability test. Those who are diagnosed with kidney disease, heart disease, or cancer and are still under active treatment. Have used other drugs, whose pharmacological effects may affect fatigue. Subjects who have systemic infections and require systemic antibiotics.