Inclusion Criteria:  The inclusion criteria for stage 3-grade C periodontitis patients were interdental CAL ≥ 5 mm and PD ≥ 6 mm on at least 6 teeth and at least three of these six teeth were not molars or incisors. They were under 35 years of age. These patients had at least two quadrants of maxilla with three teeth each having CAL ≥ 5 mm and PD ≥ 6 mm.  Exclusion Criteria:  : systemic conditions such as; diabetes mellitus, cancer, cardiovascular and respiratory diseases, current and ex-smoking habits, undergone non-surgical periodontal therapy and prescription of antibiotics or non-steroidal anti-inflammatory medication within the previous 6 months or surgical periodontal treatment within the preceding year, pregnancy, lactation, postmenopause, or immunologic disorders,