Inclusion Criteria:  Take physical examinations in our research centers and have no cancer history; "Population Health tracking Survey - simplified version of the questionnaire" must be filled according to the research program and an annual physical examination can be received as follow-up ; Timely feed back the information related to tumor diagnosis in other hospitals to the investigator during the program; Have no birth plan for the last 3 years; Fully understand the study and voluntarily sign the informed consent.  Exclusion Criteria:  Have been diagnosed with esophageal cancer, gastric cancer, colorectal cancer, liver cancer, lung cancer, pancreatic cancer, breast cancer (including non-primary, such as recurrence, metastasis or other complications) and other malignant tumors; Received blood transfusion, transplantation and other major operations within 3 months; Participated in other interventional clinical researchs within 3 months; Pregnant or lactating women; Patients with autoimmune diseases, genetic diseases, mental diseases/disabilities and other diseases considered unsuitable for the study by the investigator; Due to poor compliance, the researcher judged that the study could not be completed.