Inclusion Criteria:  Normotensive (systolic blood pressure < 130 mmHg and/or diastolic blood pressure < 85 mmHg) individuals Individuals free of cardiovascular disease and metabolic disease Individuals free of any form of autonomic dysfunction Individuals with a BMI under 30 kg/m² Women that are premenopausal with a regular menstrual cycle (26-30 days)  Exclusion Criteria:  Smokers, tobacco users (regular use in the last 6 months) Individuals with a blood pressure greater than 130/85 Subjects who use Amiodarone, Sulphaphenazole Subjects who use S-warfarin, Tolbutamine, Phenytoin, Lonafarnib Cardiometabolic medication use (e.g. anti-hypertensives, insulin-sensitizing, statins) Sex hormone replacement medical use (e.g. testosterone, estrogen, progesterone) Pregnancy