Inclusion Criteria:  Participants with achalasia Age: 18 to 80 years Accept for the treatment of POEM Sign the informed consent  Exclusion Criteria:  Participants under 18 years old Previously received treatment of achalasia, Barrett's esophagus, esophageal stricture, liver cirrhosis, and/or esophageal varices, tumors, allergic diseases and hiatal hernia. Use non-steroidal anti-inflammatory drugs, corticosteroids and other immunosuppressive agents.