Inclusion Criteria:  The patient can understand and voluntarily join the study, sign the informed consent form, and the compliance is good. Patients with lung nodule. Maximum diameter of lung nodule ≥8mm. The pathology of lung nodules biopsy was non-small cell lung cancer. I period according to the eighth edition of the TNM staging period. No mediastinal lymph node metastasis. Eastern Cooperative Oncology Group performance status of 0 to 1. No prior anticancer treatments including surgery, radiation,chemotherapy or local treatments. Sufficient organ functions.  Exclusion Criteria:  Patients with previous use of various hormones, immunosuppression or autoimmune diseases. Women during pregnancy or breast-feeding. Patients with severe heart, lung, kidney disease or other systemic diseases Patients with severe hemorrhagic diseases. Patients with a history of drug abuse or mental illness. Other conditions that the investigator has determined are not suitable for participation in this study