Inclusion Criteria:  Healthy, normal glucose tolerance test, normal blood pressure, no family history of diabetes or other metabolic history; Aged between 18 and 25; Normal body weight and waist circumference, body mass index (BMI) between 18.5 and 23.9kg/m2; No carbohydrate intolerance.  Exclusion Criteria:  Those with abnormal glucose tolerance, overweight or obese, or lactose or carbohydrate intolerance were excluded.