Inclusion Criteria:  Patient in the age group of 19-25 years . Good general health status. Healthy periodontium Absence of root resorption. Absence of any chronic disorders No history of orthodontic treatment.  Exclusion Criteria:  Presence of any signs and symptoms of gingival and periodontal diseases. Any Systemic/Bone/metabolic/hormonal disease illness known to affect the outcome of orthodontic therapy or requiring administration of vitamin D Any kind of prolonged drug administration (chronic drug intake) Pregnant or lactating women. Smoking habit.