Inclusion Criteria:  Be between 18-65 years old Have one or two focal articular cartilage lesion(s) (ICRS Grade 3 or 4A) on the femoral condyles or trochlea of the index knee; patellar tracking must be normal if the index lesion(s) is on the trochlea. Individually, a lesion can be between 2-7cm2. If more than one lesion is present, the sum of the two lesions should not exceed 10cm2. Within 6 months prior to treatment, have a Magnetic Resonance Imaging (MRI) or arthroscopic confirmation of lesion(s) in the index knee. For patients older than 40 y.o, to have a radiological Kellgren and Lawrence (K&L) grading from a standing knee radiograph taken less than 6 months previously, Grade 1 or If not, an actual test will be performed as part of the visit 1 procedures. Have a clinically stable knee, with no ligament deficiencies (<5mm side-to-side difference on Lachman and varus/valgus stress testing & Grade 0 or 1 on Pivot shift test) and the meniscal rims are intact (a maximum of 50% resection is allowed) Be able to give voluntary, written informed consent to participate and have signed an Informed Consent Form specific to this study VAS pain great than or equal to 4 in the last week. Be willing and able to comply with all study procedures including all preoperative, post-operative and rehabilitation requirements If female and of child-bearing potential, must report double-barrier, contraceptive use (e.g. use of birth-control pill and condom) for at least 2 months prior to treatment and in the 12 months following treatment. If female and of child-bearing potential, have a negative pregnancy test prior to the surgical procedure and no intention of becoming pregnant in the 12 months following treatment.  Exclusion Criteria:  Have a Body Mass Index (BMI) >35kg/m2 Have more than two lesions (ICRS grade 3 or 4A) on any surface in the index knee Have "kissing" or opposing lesion(s) (ICRS grade 3 or 4A) of the condyle, tibia or patella in the index knee; additional linear lesions which bear Grade 3 or 4 characteristics may be acceptable if they are determined by the Investigator to be incidental, not clinically relevant, and consistent with the subject demographic Have malalignment of >5 degrees varus or valgus in the index knee based on standard AP x-rays requiring an osteotomy Have had any surgical treatment for cartilage repair in the index knee within 1 year prior to treatment Have had intra-articular injections within 3 months in the index knee Have a diagnosis of a concomitant knee injury which may confound assessment of the index knee (e.g., important meniscal injury) Have significant pain emanating from other lower body joints in the ipsilateral (hip, ankle) or contralateral (hip, knee, ankle) limb. Have known allergies to shellfish Have a known history of crystalloid or inflammatory arthropathy Have any condition that is unrelated to the index knee and significantly impairs walking ability (e.g., spinal stenosis, sciatica) Have advanced musculoskeletal disease Have active coagulation disorders Are currently using antibiotics Are participating concurrently in another clinical investigation, or have participated in a clinical investigation within the last 90 days, or intend to participate in another clinical investigation during the course of the study Are currently abusing drugs or alcohol or have a history of the same within the last 12 months Have any mental or psychological disorder that would impair their ability to complete the study questionnaires Are currently breastfeeding or planning to breastfeed any time during the course of the study Are currently a prisoner Have significant comorbidities or conditions associated with high-risk for surgical or anesthetic survival (e.g., renal failure, peripheral vascular disease, unstable cardiac disease, poorly controlled diabetes, immunosuppression, etc.) Have any medical condition or other circumstances, in the judgment of the investigator, that might interfere with the ability to return for follow-up visits, including any systemic illness, neuromuscular, neurosensory, or musculoskeletal deficiency that would render the subject unable to perform appropriate post-operative rehabilitation Have any condition which, in the judgment of the Investigator, would preclude adequate evaluation of the device's safety and performance  Intra-operative Exclusion Criteria:  Subjects who meet any of the following intra-operative screening criteria will be excluded from participation in this study:  Have lesion(s) in the index knee that are not contained (intact shoulders) and/or exceed 7 cm² in total size after debridement for a single lesion and 10cm2 for more than one lesion, if within the same condyle or the trochlea. If only a single lesion suitable to be included is identified, it might be between 2-7cm2.  Minor concomitant procedures are allowed such as, but not limited to:  Removal of loose bodies Plica excision Minor synovial removing Minor chondroplasty (debridement) Lysis of adhesions Meniscal trimming/suturing which respects the exclusion criteria.