Inclusion Criteria:  Code equal of higher than 2 (Basic Periodontal Examination-BPE). I or II ASA classification. Without periodontal treatment on past three months.  Exclusion Criteria:  Acute infection Dental trauma Alveolar osteitis (Dry socket) Elderly dependent patients Orthodontic appliances Antibiotic therapy Analgesic therapy Heavy smokers Menstrual period Pregnants Lactating woman