Inclusion Criteria:  healthy volunteer aged 3~59 years old； no vaccination with any influenza vaccine within the past year； no catching any influenza or flu like symptoms （axillary temperature ≥ 38℃， accompanied by cough or sore throat ）； influenza susceptible individual（any serum Hi antibody level of H1N1, H3N2 and B≦1:10）； with informed consent obtained from the volunteer and / or guardian； willing of volunteers and / or guardians to comply with the requirements of the clinical trial protocol.  Exclusion Criteria:  Known allergy individual to any ingredient of this product, including egg protein, subsidiary material and/or gentamycin sulfate; patients with acute disease, severe chronic disease, acute attack of chronic disease and/or fever (axillary temperature ≥ 37.3℃ on the day of immunisation); pregnancy (enquire); patients with Leigh syndrome treated with aspirin or aspirin containing drugs; patients with immunodeficiency or receiving immunosuppressive therapy; patients with uncontrolled epilepsy and other progressive nervous system diseases, with a history of Guillain Barre syndrome; patients with rhinitis or other nasal abnormalities judged by clinicians that may affect vaccination or sample collection; accept any immunoglobulin and / or any blood products within 3 months, or plan to use them during the study (until post immunization blood sample collection); .taking anti influenza drugs in the past 48 hours; .any situation that the researchers believe may affect the study.