Inclusion Criteria:  participants of both genders aged between 12 and 35 years, acne grade II and III acne mixed or oily skin phototype between I and IV, according to the Fitzpatrick scale, were recruited  Exclusion Criteria:  Pregnant or lactating women, as well as those intending to become pregnant during the study period patients who received treatment with corticosteroids and antimicrobials within 30 days prior to selection patients who were treated with immunosuppressants within 90 days prior to selection.