Inclusion Criteria:  18-55 years; healthy subjects, patients with AR without AIT, or patients with AR with AIT for more than 1 year  Exclusion Criteria:  who infected with COVID-19 previously Cannot finish the follow up Previous allergic to other vaccines who have had severe immunologic, cardiac, liver or metabolic disease, tumors, allergic diseases, or chronic infection, pregnancy or breastfeeding Suffered from airway infection or severe infectious diseases in the past 3 months, prior to this study.