Inclusion Criteria:  Expected poor responder based on:  AMH: 0.3-1.2 ng/ml or AFC <5, AMH>1.2 ng/ml or AFC≥5 but the retrieval of ≤ 4 oocytes during previous IVF treatment. motile sperm with normal morphology obtained from the ejaculate of testicular biopsy no more than 3 previous failed IVF cycles (if the patient had 2 or more previous cycles cancelled for poor response she cannot be included) BMI: 18-35 kg/m2 regular 24-35 day cycles intact uterine cavity indication for in vitro fertilisation treatment (tubal factor, male factor, low ovarian reserve, endometriosis, unexplained infertility) age 18-421 yrs  Exclusion Criteria:  presence of hydrosalpinx positive HIV, hepatitis B, C screening tests planned preimplantation genetic testing of the embryos planned elective cryopreservation lack of consent not meeting the inclusion criteria