Inclusion Criteria:  Women aged 20-65 years old; Have a history of sexual life; The patients were voluntarily enrolled in the group and signed an informed consent form.  Exclusion Criteria:  History of cervical conization, pelvic radiation and hysterectomy, acute inflammation of the reproductive tract, severe system disease or other malignant tumors; Pregnant and lactating women; The patient's compliance is poor or the researcher thinks it is not suitable for this study.