Inclusion Criteria:  Livers from donor donor brain death with informed consent before inclusion in the study was obtained from all participants or families.  Exclusion Criteria:  Age < 18 years old Donor after cardiac death Split Cholestasis due to a biliary obstruction Total bilirubin levels above 2,5 mg/dL Glutamic oxaloacetic transaminase (SGOT)/ serum glutamatepyruvate transaminase (SGPT) levels and gamma-glutamyl transaminase (GGT) levels above 400 U/L Cirrhotic livers