Inclusion Criteria:  age 25-55 years Body mass index:> 18 and <30 kg / m2 Stable body weight for at least 3 months regular practice of physical activity for at least 2 years  Exclusion Criteria:  Chronic diseases (cardiovascular, hepatic, respiratory, cancer) Acute inflammatory states, infections or other pathologies Regular use of probiotics Use or treatment with steroids (within the previous 3 months)