Inclusion Criteria:  1.18 years or older  2.Meet 2 of 4 sepsis criteria  Temperature > 38C or < 36C Heart rate > 90 bpm Respiratory rate >20 or PaCO2 <32mmHg  WBC >12000/µL or < 4000/µL or > 10% bands  3.Hospitalized patients who have a diagnostic blood culture ordered as standard of care for suspected sepsis  4.Informed Consent by patient or legal representative.  Exclusion Criteria:  Refusal to participate in the study or Failure to comply with treatment or follow-up time Known breastfeeding or pregnancy The researcher believes that there are any conditions (social or medical) that allow subjects to participate is unsafe. For example, severe anemia or high risk of bleeding,etc., which are not suitable for taking peripheral blood for testing Participating in other clinical studies