Inclusion Criteria:  Total of 21 patients enrolled in this study will be selected having at least 4 teeth (one on each quadrant) which will be extracted for orthodontic treatment. These patients will be selected according to age groups between 15-25 years old good oral hygiene free of destroying habits for dental tissues  Exclusion Criteria:  Bruxism soft drinks consumption acid regurgitation, etc.). Selected teeth having any cracks, malformations, dysplasia or caries.