Inclusion Criteria:  18-85 years old, regardless of gender; Patients with newly diagnosed CRC who are planned to undergo surgery (including patients with metastases but still feasible for surgery); Patients who have not been diagnosed for the first time and have not received surgical treatment and now planned to undergo surgery(e.g., patients after neoadjuvant radiotherapy and chemotherapy for middle and low rectal cancer).  Exclusion Criteria:  Patients who refused to sign informed consent; Patients complicated with other tumors; Pregnant or lactating women; People with a history of psychotropic drug abuse and unable to quit or those with mental disorders; Postoperative histopathological examinations for patients with non-CRC type; Patients who could not be followed up.