Inclusion Criteria:  Male over 18 years-old Normal spermogram (concentration ≥15 million / ml or total count> 39 million, progressive mobility ≥32% or total mobility ≥40%) Progressive mobility ≥70% after selection Total number of progressive motile spermatozoa recovered after TMS> 10 million Patient affiliated or beneficiary of a social security scheme Patient having been informed and not opposing to this research.  Exclusion Criteria:  Male over 65 years-old Protected patient: adult under guardianship, curatorship or other legal protection, deprived of liberty by judicial or administrative decision