Inclusion Criteria:  Age 18-75 Meeting ICD-10 depressive episode criteria (F32, F33, F34, F38, F39) Cultural, social and educational background sufficient to understand informed consent and research content Agree to participate in this study  Exclusion Criteria:  Exclude other mental disorders such as bipolar disorder and schizophrenia (but not comorbidities) Patients with a history of brain injury or cerebrovascular accident, myocardial infarction, severe liver cirrhosis, acute and chronic renal failure, severe diabetes, aplastic anemia, moderate and severe malnutrition and other serious physical diseases of the nervous, heart, liver, kidney, endocrine and blood systems