Inclusion Criteria:  Healthy persons between the ages of 18 and 45 years Mandibular molar teeth that were diagnosed with symptomatic irreversible pulpitis  Exclusion Criteria:  Patients who are taken analgesic inflammatory drugs with in the last 12 hours Pregnancy or lactation Teeth with calcified canals Teeth with periodontal diseases Teeth with sensitive to percussion and palpation Teeth with root resorption Teeth with immature/open apex