Inclusion Criteria:  Male patients Aged from 30 to 60 years old Systemically healthy Having at least 20 teeth Diagnosed with moderate to severe periodontitis according to american academy of periodontology 2015: gingival bleeding at examination, ≥5mm periodontal pocket depth (PPD), bone resorption on x-ray film ≥16% or >3mm root length Having at least two sites at anterior teeth with PPD from 5 to 7 mm For group 1, subjects who never smoke For group 2, subjects who had smoked more than 10 cigarettes per day for at least the past 10 years  Exclusion Criteria:  Patients with acute or chronic medical disorders Patients under any medication for the past 3 months Patients who had undergone periodontal therapy in the last 12 months Patients with oral lesions such as ulcers, aphthous ulcers, glossitis or multi tooth decays