Inclusion Criteria:  Males or females at least 18-70 years old. Patient able to give informed consent. Patient with biopsy-proven CRC Patient with biopsy-proven Crohn's disease (CD) or ulcerative colitis(UC), in the active phase. patients with clinical diagnosed DM2 patients with ACVD:coronary angiography more than 50% stenosis in single or multiple vessels. The age- and sex- matched health controls for each patient.  Exclusion Criteria:  1.Use antibiotics or probiotics within the last 2 weeks.