Inclusion Criteria:  According to the American Society of Anesthesiologists (ASA) only status 1/ healthy patients Completely erupted permanent dentition, No fixed orthodontic apparatus, prosthetic restoration and filling material, A minimum age of 18 years, At least one third erupted molar teeth (third mandibular or third maxillary molar from each patient was included), Non cavitated occlusal caries, Teeth with no hypoplasia or hypomineralization, Third molar teeth that require extraction for surgery, orthodontics and prosthetic reasons, If clinical indicators suggest a current caries risk or activity that required additional diagnosis, the patients will be asked to participate in this additional examination.  Exclusion Criteria:  Patients younger than 18 Patients that can not be recruited according to the inclusion criteria.