Inclusion Criteria:  age between 18 and 80 years old life-expectancy at least 10 years fully understand the clinical trial protocol and sign the informed consent;  Exclusion Criteria:  Already diagnosis with prostate cancer; cannot tolerate prostate biopsy or has contraindication to biopsy; cannot tolerate prostate biopsy or has contraindication to MRI examation; undergo biopsy within 1 year patients judged by the investigator to be unsuitable to participate in the clinical trial;