Inclusion Criteria:  diagnosis of alcohol dependence according to ICD-10 and DSM-V aged between 20 and 65 Ability to perform oral and written informed consent to the study protocol  Exclusion Criteria:  age under 20 or over 65 years taking medication affecting the pupillary measurements (opioids, acetylcholinesterase inhibitors, sympathomimetics) positive pregnancy test diagnosed anxiety disorders multiple substance abuse disorder ocular disease delirious patients biochemical implants hemophilia psoriasis vulgaris