Inclusion Criteria:  Voluntary signing of informed consent. Age 18-75 years old (inclusive), regardless of gender. Pathologically proved to be malignant melanoma, including skin, mucous membrane, and limb origin, and locally progressive or metastatic melanoma that cannot be completely resected. The patient has more than 1 superficial focus for intratumoral injection (suitable for direct or ultrasound-guided injection), which can be skin, subcutaneous or lymph node focus, the longest diameter is (LD) ≥ 1cm and ≤ 10cm, and there is no ulcer in the injected focus.  Patients who have failed to receive first-line treatment of melanoma in the past, including chemotherapy and targeted therapy.  Systemic anticancer therapy or adjuvant therapy must be completed at least 4 weeks before enrollment (if previously received nitrosourea or mitomycin C chemotherapy, the interval between the end of chemotherapy and the study group is more than 6 weeks).  Patients with adverse events caused by previous treatment recovered to ≤ CTCAE level 1, except for hair loss.  Based on RECIST 1.1, imaging evaluation confirmed that there was an assessable focus (except for one measurable lymph node focus). The ECOG physical condition score is 0 or 1, and the expected survival time is more than 3 months. The functions of blood, liver and kidney are good and meet the results of laboratory tests:  1) Absolute neutrophil count (ANC) ≥ 1.5x10^9 / L. 2) Platelet ≥ 80 × 10^9 shock L. 3) Hemoglobin ≥ 90g/L. 4) Serum albumin ≥ 28g/L. 5) Bilirubin ≤ 1.5x ULN. 6) ALT and AST ≤ 2.5x ULN, if there is liver metastasis, ALT and AST ≤ 5x ULN. 7) Serum Cr ≤ 1.25 × ULN or endogenous creatinine clearance ≥ 50mL/min (using standard Cockcroft-Gault formula).  8) Coagulation function, in which INR < 1.7. 9. Female or male patients of childbearing age should take effective contraceptive methods during the study period and within 3 months after the end of the study treatment period. Non-operative sterilization female patients of childbearing age must be negative for serum or urine HCG test during the screening period.  Exclusion Criteria:  Any active autoimmune disease or history of autoimmune disease (such as, but not limited to, interstitial pneumonia, uveitis, enteritis, hepatitis, rheumatoid arthritis, nephritis, pituitary inflammation, hyperthyroidism, hypothyroidism, etc.); subjects with vitiligo or childhood asthma still need medical intervention after adulthood.  Asthma in which subjects need bronchodilators for medical intervention.  Subjects were treated with immunosuppressants or systemic or absorbable local corticosteroids within 2 weeks before enrollment to achieve immunosuppression (dose > 10mg/ prednisone or equivalent). Has received or plans to receive any form of organ transplant, including allogeneic stem cell transplantation. Known to be allergic to macromolecular protein preparations, or to any composition of ZKAB001 and Pexa-Vec. Severe allergic reactions or side effects occurred in the past vaccination process. If you have other malignant tumors at the same time, the survival without progression or recurrence should be more than 3 years.  Subjects with known active central nervous system (CNS) metastasis and / or carcinomatous meningitis.  Note: subjects who have previously received brain metastasis treatment and subjects with clinically stable CNS metastasis may be allowed to participate in the study (defined as at least 4 weeks before the first dose of the trial treatment: (1) No new or expanded evidence of CNS metastasis (through MRI); (2) subjects have been treated without hormones for at least 2 weeks; (3) any neurological symptoms have returned to baseline levels.  Patients with uncontrolled cardiac clinical symptoms or diseases, such as heart failure above NYHA2, unstable angina pectoris, myocardial infarction within 1 year, clinically significant supraventricular or ventricular arrhythmias requiring treatment or intervention, left ventricular ejection fraction at rest was less than 50%. After injection treatment, tumor swelling and / or necrosis may invade important vascular structures (such as carotid artery) or other important anatomical sites (such as spinal cord). A history of severe inflammatory skin diseases requiring drug treatment and a history of severe eczema requiring drug treatment (confirmed by researchers). The subjects had active infection, or had a fever of unknown origin during the screening period or within a week before the first administration of the drug > 38.5 ℃. (according to the judgment of the researchers, the fever caused by the tumor can be included in the group.).  twelve。 Active hepatitis B or C (unless HBV-DNA titer < 500IU/mL or copy number < 1000copies/ml, HCV-RNA negative can be included in), HIV [G1] positive or known history of acquired immunodeficiency syndrome after antiviral treatment.  13. Subjects are participating in other clinical studies, or less than one month from the end of the previous clinical study, except for non-interventional clinical studies (such as epidemiological studies).  14. HCV therapy could not be stopped within 14 days before Pexa-Vec injection. If a patient is using any antiviral drugs, he or she should consult the researcher to determine whether the patient is suitable to participate in the study.  15. Less than 4 weeks from the previous use of immune checkpoint inhibitors, such as PD-1/CTLA-4 inhibitors (that is, more than 4 weeks from the previous receipt of PD-1/CTLA-4 inhibitors); or have been treated with other oncolytic viruses (such as T-Vec, recombinant human GM-CSF herpes simplex virus injection (OrienX010), etc.) or PD-L1 inhibitors.  16. The patients were vaccinated with live vaccine within 4 weeks before screening.  Inactivated vaccines to prevent influenza and inactivated vaccines to prevent other infectious diseases can be used on a case-by-case basis, but it is necessary to consider increasing the elution period before and after medication.  17. Subjects are known to have a history of psychotropic substance abuse, alcohol abuse or drug use.  18. Have a history of pulmonary tuberculosis. 19. The researchers evaluated that there was a significant bleeding event in the previous 12 months and the risk of intratumoral injection procedure increased.  20. Pregnant or lactating women. 21. The researchers determined that the patient had other factors that might lead to the termination of the study, such as other serious diseases or serious laboratory abnormalities or accompanied by other factors that would affect the safety of the subjects, or family or social factors such as the collection of trial data and samples.