Inclusion Criteria:  - For test groups  females of reproductive age group (15-40 yrs) BMI (18.5 - 24.9) The diagnosis of PCOS according to Rotterdam criteria(37), when any two out of the following three abnormalities will be present:  clinical (hirsutism, acne or acanthosis nigricans) and/or biochemical (raised testosterone) hyperandrogenism chronic anovulation (oligomenorrhoea or amenorrhea) polycystic ovaries on ultrasound (one or both ovaries demonstrate 12 or more follicles measuring 2 to 9 mm in diameter or the ovarian volume exceeds 10 cubic cm on pelvic ultrasound)  presence of ≥20 natural teeth Stage I/II periodontitis by using the criteria laid down by World Workshop 2017  For Test Group 2  Participants will receive professional oral hygiene instructions during the trial and will be given cause related treatment after observation period of 6 months. Delaying treatment will not be considered unethical because of the low risk of disease progression over 6 months (41) and because these individuals will be monitored frequently and offered immediate (rescue) treatment if their disease progresses. Such cases will be excluded from the study. Periodontitis progression will be defined as an increase in CAL of 2 mm at any tooth site(42). Participants will be enrolled in the present study after obtaining written informed consent after explaining the details of the study design in their native language. Also, patients with grade C periodontitis or molar incisor pattern of periodontitis (2017 criteria) will not be included in the study For control group Systemically healthy females (age and BMI matched with PCOS women) diagnosed on the basis of regular menstruation, no clinical or biochemical sign of hyperandrogenism and ultrasound exclusion of PCOS, having stage 1/2 periodontitis.  Exclusion Criteria:  • history of androgen-secreting tumors, congenital adrenal hyperplasia and thyroid dysfunction  nephrotic syndrome, chronic renal failure, significant cardiovascular disease, established type 1 or type 2 diabetes mellitus, active cancer within the last past 5 yrs smokers and alcoholics history of systemic antibiotics or oral contraceptives usage within last 3 months periapical pathology or oral inflammatory conditions other than periodontitis. any periodontal treatment within 6 months prior to study incisor molar pattern/grade C periodontitis (2017 classification)