Inclusion Criteria:  Apparently healthy (self-reported) aged between 20 and 35 BMI between 18.5 to 27 kg/m2 Recreationally active, defined as minimal of 3 hours training per week, and a maximum of 10 hours a week  The sport a participant is engaged in should be a game sport, endurance sport, or should at least contain a major endurance component. Examples of sports that can be included are: distance running, cycling, triathlon, crossfit, and game sports like soccer, rugby, handball, hockey,  ○ For part 2 subjects should be used to prolonged cycling exercise  Able to be present and participate at all test days and willing and able to follow prescribed interventions  Exclusion Criteria:  Inactive (defined as <3 hours of training per week) Non-recreational athlete (>10 hours of training per week) active in a sport without a major endurance component Smokers, defined as someone who has smoked regularly in the previous year. Excessive alcohol consumption (i.e. more than 14 units for males per week; 7 units for females) Drug use The more 'extreme' diets (like veganism, ketogenic diet, raw-food, carnivore diet etc.) Participation in another biomedical study possibly interfering with the study results within 1 month before the first study visit Inability to participate and/or complete the required measurements People working for the department of Human Nutrition and Health (including Master thesis students)