Inclusion Criteria:  PORs who meet at least two of the following three Bologna criteria AMH: 0.1-1 ng/ml.  Exclusion Criteria:  ovarian insufficiency due to gonadal dysgenesis and - chromosomal abnormalities, immunoglobulin A deficiency, the use of anticoagulants, psychotropic medicaments, psychiatric disorders, carcinomas or a history of chronic pelvic pain. Women with present infection, haemoglobin lower than 11 g/L or platelets lower than 150 x 10ʒ/μL were excluded from the study.