Inclusion Criteria:  healthy subjects; men and women; age 18-50 years; 20 kg/m2 ≤ BMI ≤ 35 kg/m2; habitual daily breakfast consumption (≥ 250kcal/day); habitual diet characterized by absence of any food supplements and alternative medication, probiotics and prebiotics, whole grain and/or fiber enriched foods; intake of fruit/vegetables < 3 servings/day; low level of physical activity; signed written informed consent.  Exclusion Criteria:  food allergies and intolerances and celiac disease; gastrointestinal diseases; relevant diseases; pregnant or breastfeeding; previous abdominal surgery; hypertriglyceridemia (Triglycerides > 200 mg/dL); hypercholesterolemia (Total cholesterol > 200 mg/dL); hyperglycaemia (glycaemia ≥ 110 mg/dL); hypertension (arterial blood pressure ≥140/90 mm Hg); weight loss ≥ 3 kg within 2 months before the study; antibiotics treatment within 3 months before the study; any medication (different from antibiotics) at the enrollment and within 2 months before the study; habitual diet characterized by high fruit and vegetables intakes (>3 portion/die); high level of physical activity; alcohol consumption ≥ 3 alcohol units per day; simultaneous participation in other trial.