Inclusion Criteria:  Age between 18 and 45 years old Type 1 diabetes diagnosed at least 1 year before the inclusion in the study. Diagnosis confirmed by positive autoimmunity (GAD-65 or IA2) and insulin deficiency. Treatment with subcutaneus insulin therapy (multiple dose or continuous subcutaneous insulin infusion). Menarche at least 2 years before the study.  Exclusion Criteria:  Honey moon period. Altered thyroid hormone or prolactin levels. Congenital adrenal hyperplasia. Severe chronic disease. Oral contraceptive or glucocorticoid therapy in the previous 3 months.