Inclusion Criteria:  Patients undergo emergency renal transplantation under general anesthesia(CLCR≤30ml/min),or patients undergo elective surgery in supine position(CLCR≥80ml/min) Patients age 18 to 65 ASA glass I-III  Exclusion Criteria:  patients suffering obstructive sleep apnea hypopnea syndrome or BMI≥28kg/m2 Pregnant or lactating women neuromuscular disorders, hepatic dysfunction, cardiac disease, a history of malignant hyperthermia, or allergic history during general anesthesia patients receiving aminoglycoside antibiotics, anticonvulsants, magnesium,fusidic acid,toremifene and/or flucloxacillin