gout  Inclusion Criteria:  age:year of 25~60; Be consistent with the 2015 American College of Rheumatology/ European League Against Rheumatism (ACR/EULAR) gout diagnostic criteria ,and serum uric acid >420 μmol/L; The patient are willing to take part in our study.  Exclusion Criteria:  abronia, surgery, postoperative, dehydration within 2 weeks, acute or chronic infectious diseases, severe trauma, malnutrition, diabetes, malignant tumors; diagnosed as cardiovascular and cerebrovascular diseases within 3 months; heart failure (New York Heart Academy，grade IV); hepatic insufficiency ( Alanine transaminase or Aspartate aminotransferase ≥3×upper limit)； chronic kidney disease or abnormal renal function( serum creatinine ≥1.5×upper limit)； excessive drinking； The investigators considered the patients unsuitable for inclusion in this study.  hyperuricemia:  Inclusion Criteria:  age:year of 25~60; A medical record in our hospital showed that the person is healthy, without key disease; serum uric acid >420μmol/L without gout flares. Exclusion Criteria:Same as the group of gout.  healthy controls:  Inclusion Criteria:  age:year of 25~60; A medical record in our hospital showed that the person is healthy, without key disease; serum uric acid ≤420μmol/L. Exclusion Criteria:Same as the group of gout.