Inclusion Criteria:  Men more than 18 years old with clinical suspicion of prostate cancer; Serum PSA ≤ 20 ng/ml within the previous 3 months; No evidence of PSA increase by noncancerous factors, such as catheterization, bladder stones, or urinary tract infection including bacterial prostatitis; mpMRI PI-RADS V2.1 score ≥4, diameter of ≤2.5 cm； Able to provide written informed consent.  Exclusion Criteria:  Prior prostate biopsy or prostate surgery; Prior treatment for prostate cancer; Contraindication to MRI (e.g. claustrophobia, pacemaker); Contraindication to prostate biopsy; Men in whom artifact would reduce the quality of the MRI.