Inclusion Criteria:  Sign the informed consent form voluntarily. Age 18-70 years old. High expression (positive) group: The results of multiple laboratory tests indicate that the marker is higher than the normal range. Low expression (negative) healthy volunteer group: those with no underlying diseases and no abnormalities in the physical examination. Have routine examination to check the remaining body fluid samples, and the body fluid samples are not contaminated.  Exclusion Criteria:  Accompanied by other inflammations. The data of the subject is not complete, or the collection of cases is incomplete due to other reasons. The researcher judged that it was not suitable to participate in this research.