Inclusion criteria:  Age from 32 to 60 years old. Females and males. Healthy adult patients. Selected patient with a single site of defect in single tooth:  Two walled or three walled infra-bony defect. The bone defect should be at least 3 mm in depth from the crest of the alveolar bone to the base of the defect.  Pocket depth of more than or equal to 5 mm. Clinical attachment loss equal or more than 3mm.  Exclusion criteria:  Smokers. Breast feeding and pregnant females. Vulnerable group of patients (handicapped and prisoners). Patients who have received any periodontal therapy or antibiotics in the last 6 months.  The study protocol explained in details to all patients. Then a signed informed consent obtained from the patients. The data obtained from patients as well as the results of the follow up will be kept confidential.