Inclusion Criteria:  • The study includes female patients with chronic knee OA  The study includes patients from the age of 40 years and 65 years The study includes patients with Grade II and III OA of knee Patients having no other physical and mental issues  Exclusion Criteria:  • The study excludes male patients  Patients with lab results abnormal (inflammatory and infectious disease, malignant tumor) Patients with arterial coagulation blockage in lower limbs. Usual contraindication for laser therapy Patient's undergone surgery of knee