Inclusion Criteria:  Voluntarily agree to participate in the study and sign an informed consent; Are on a regular haemodialysis (HD) program with three weekly sessions; AVF has been in use for at least 4 weeks without incident; AVF with blood flow (Qa) ≥500 mL/min evaluated by thermodilution; AVF paths allow cannulations along the entire length of the vein with at least 6 cm of distance between bevels, or two distinct areas of 3 cm in length; Adult patients  Exclusion Criteria:  Those who decline to take part; Those who have undergone angiography or surgical intervention in the last 4 months in the AVF in use; Those who have undergone three or more interventions in the AVF in use; Those with use of anesthetic creams at cannulation sites.