Inclusion Criteria:  Adult patients of both sexes (age ≥ 18 and ≤ 60 years) Physical state ASA I or II according to the criteria of the American Association of Anesthesiology Planned surgery of cholecystectomy by videolaparoscopy  Exclusion Criteria:  Patient's refusal to participate in the study Inflammation or infection in the sublingual mucosa Emergency surgery Grade III obesity (BMI ≥39.9 kg/m2) Pregnancy or lactation Autoimmune diseases, malignant neoplasms