Inclusion Criteria:  adult critically ill patients admitted to the ICU in Minia University Hospital. Patients Ages was ≥ 18 and ≤ 65 years including both sexs, resuscitation started after fulfilling the diagnostic criteria of sepsis issued by Surviving Sepsis Campaign 2016  Exclusion Criteria:  We excluded Patients with chronic organ failure (Cardiovascular, Respiratory, Liver, Neurological and Renal.)