Inclusion Criteria:  Patients are not diagnosed with glaucoma and don't receive any ocular hypotensive eye drops at our clinic. Patients accept the rules for follow-up visits for at least six months.  Exclusion Criteria:  Patients are with a history of ocular trauma. Patients are with contact lens use. Patients are diagnosed with secondary glaucoma. Patients have significant diseases affecting the meibomian gland and ocular surface. Patients have poor IOP control that required additional ocular hypotensive eye drops.