Inclusion Criteria:  patients with peripheral lung lesion suspected of lung cancer detected on the chest computed tomography (CT) and RP-EBUS scans Control group - patients without non-small cell lung cancer Written (signed) Informed Consent to participate in the study  Exclusion Criteria:  severe hypoxemia (partial pressure of oxygen (pO2)< 60 mmHg) or hypercapnia (carbon dioxide partial pressure (pCO2)> 50 mmHg), bleeding disorders The diffusing capacity for carbon monoxide (DLCOc)< 35 %, forced expiratory volume in one second (FEV1) < 800 ml or FEV 1 < 30 %, peripheral lung tumor was not visualised by RP-EBUS longer than 30 minutes large vessels (more than 3 mm) near the tumor on the CT scan, technically difficult to introduce cryoprobe and/or endobronchial blocker to current bronchi segment or subsegment, excessive bleeding after transbronchial forceps biopsy, which was needed extra intervention to stop bleeding.