Inclusion Criteria:  18-60 years of age; Lives in Lithuania; Able and willing to give informed written consent to participate in the study; Recent (24 h before volunteering for the study) ankle injury requiring 6-week ankle immobilization; Exercise training program during 6-week recovery is recommended.  Exclusion Criteria:  Body mass index (BMI) is greater than 30 kg / m2; Diabetes complicates cardiovascular diseases, Liver disease; Blood pressure is greater than 150/90mmHg during the first measurement; Incidences of consciousness loss; Cancer; Dementia; Other injuries that affect lower limb muscles; Use of anticoagulant drugs; Smoking; Drug abuse; Using drugs that affect muscle function (steroids).