Inclusion Criteria:  Ages ranged from 18 to 80. ASA # or # level.  Exclusion Criteria:  Allergy or contraindication to remifentanil or rocuronium; required for Central venipuncture catheterization; Abnormal liver or kidney function; Heavy drinking and long-term use of sedatives, analgesics or anti-anxiety drugs; Hearing and language impairment; Peripheral vascular disease; Severe cardiovascular disease or neurological disorders; Failure of one-time peripheral venipuncture; Infection of hand or wrist skin.