Inclusion Criteria:  - 1. Patients who are recently diagnosed with CRC based on clinical and pathological examinations.  2. Control subjects will be CRC-free, based on the clinical history and physical examination.  Exclusion Criteria:  1 - Patient refuses to be enrolled in the study. 2. Subjects with cancer metastasized from other tissues to the colon. (colon is the site of metastasis)