Inclusion Criteria:  Male or female subjects aged 18 to 65 years, inclusive, at the time of consent. Subject is willing to participate and is capable of giving informed consent. Subject must be willing to comply with all study procedures and must be available for the duration of the study. CHE subjects: has moderate to severe chronic hand eczema described as IGA score of 3 or greater.  Healthy volunteers: in good general health.  Exclusion Criteria:  Subject has a history of skin disease or presence of skin condition that, in the opinion of the investigator, would interfere with the study assessments. Subject has used topical antibiotics within 1 week prior to Day 1. Subject has received any marketed or investigational biological agent within 12 weeks or 5 half lives (whichever is longer) prior to Day 1. Subject is currently receiving a nonbiological investigational product or device or has received one within 4 weeks prior to Day 1.