Inclusion Criteria:  Age ≥ 18 TBSA≥ 20% （TBSA，the total burn area.） Admitted to hospital within 24 hours after injury.  Exclusion Criteria:  STB ≥ 33 μ mol/L Cr ≥ 171 μ Urine output l< 500 ml / 24 h Pregnant and lactating women Severe combined injury Glucocorticoids users Immunosuppressants users Patients survived less than 48 hours