Inclusion Criteria:  The inclusion criteria corresponding to the risk group consist of:  Participants that present hypertension or diabetes mellitus before pregnancy BMI >35 Family history of preeclampsia or diabetes Macrosomic previous baby BMI< 20 History of pretem delivery Previous intrauterine growth restriction or gestational mellitus diabetes history Maternal age <16 years or > 40 years History maternal infection.  The inclusion criteria for healthy participants are:  Patients between 10-12 weeks of gestation BMI between 20 to 30.  Exclusion Criteria:  Exclusion criteria are: Participants consuming multivitamin complexes Unable to sign inform consent.