Inclusion Criteria:  Aged 18 to 65 years Patients of NPM  Exclusion Criteria:  Pregnancy Lactation Cigarette smoking Alcohol addiction Hypertension Diabetes mellitus Lipid dysregulation BMI > 27 <18.5 Recent (< 1 months prior) use of antibiotics, probiotics, prebiotics, synbiotic, hormonal medication, laxatives, proton pump inhibitors, insulin sensitizers or Chinese herbal medicine History of disease with an autoimmune component, such as multiple sclerosis(MS), rheumatoid arthritis, irritable bowel disease(IBS), or irritable bowel syndrome(IBD) History of malignancy or any gastrointestinal tract surgery