Inclusion Criteria:  systemically healthy non- smoker, not received any periodontal treatment within the last 6 months, no antibiotics, anti- inflammatory drugs or any other medication taken within the last 6 months  Exclusion Criteria:  any systemic disease that might influence the prognosis of periodontal disease and outcome of the treatment, any medical condition that required antibiotic prophylaxis before the treatment, smoking, pregnancy and lactation, ingestion of antibiotics, anti-inflammatory drugs or any other medication during the study period, any physical limitations or restrictions that might preclude normal oral hygiene procedures.