Inclusion Criteria:  Male and female ≥ 18 years old; The history showed that the last clinical diagnosis of ischemic stroke was more than 6 months; The results of MRI at the first diagnosis and at the time of selection indicated that there was ischemic stroke and dysfunction; There was no significant improvement in neurological function or functional defect 2 months before the study; There is serious neurological dysfunction related to the diagnosis in Article 2, which leads to the subjects need the assistance of others to walk, or cannot complete the general activities of daily living independently; NIHSS score was 6-20; The life expectancy is more than 12 months; Before treatment, the patient received standard medical care for secondary prevention of ischemic stroke, including but not limited to appropriate blood pressure and cholesterol control measures, use of antiplatelet drugs or anticoagulants (except prohibited cases); Be able to understand and provide the signed informed consent, or ask the designated legal guardian or spouse to make the above decision voluntarily on behalf of the subjects; It is reasonable to expect that patients will receive standard medical care for secondary prevention of ischemic stroke and participate in safety follow-up of all plans; Organ function determined according to the following criteria:  Serum AST ≤ 2.5 × Upper normal limit (ULN);  Serum alanine aminotransferase (ALT) ≤ 2.5 × Normal upper limit;  Total serum bilirubin ≤ 1.5 × Normal upper limit;  In subjects without antithrombotic therapy, prothrombin time (PT) and partial thrombokinase time (PTT) ≤ 1.25 × Normal upper limit;  Serum albumin ≥ 3.0g/dl;  Absolute neutrophil count (ANC) ≥ 1500/ μ L；  Platelets ≥ 150000/ μ L；  Hemoglobin ≥ 9.0g/dl;  Serum creatinine ≤ 1.5 × Normal upper limit;  Serum amylase or lipase were in normal range.  Exclusion Criteria:  History of epilepsy; History of tumor; History of brain tumor and brain trauma; hepatitis B, five surface antigens, e antigens, e antibodies and core antibodies were positive for any one, positive for hepatitis C virus antibody, positive for syphilis serum antibody or HIV positive. Myocardial infarction occurred within 6 months before the trial; Suffering from any other medical disease with clinical significance, or with abnormal mental or laboratory results, the researcher or the sponsor determines that participating in the trial will bring safety risks to the subjects; Imaging examination showed subarachnoid hemorrhage or intracerebral hemorrhage in the past 12 months; Participate in another study on the use of test drug or equipment within 3 months before treatment; Participated in other stem cell therapy related research; History of drug or alcohol abuse in the past year; Women who are known to be pregnant, breast-feeding or have a positive pregnancy test (to be tested during the screening process) or plan to be pregnant during the trial; Allergic to cattle and pork products.