Inclusion Criteria:  People requiring lumbar puncture testing due to brain trauma, intracranial infection, intracranial vascular disease, intracranial tumors, peripheral neuropathy for the raised intracranial pressure group. Healthy people for the normal intracranial pressure group.  Exclusion Criteria:  eye diseases, such as eye trauma, eye tumors, optic neuritis, and glaucoma People with poor temporal window penetration. Patients who refuse to sign informed consent.