Inclusion Criteria:  Samples with Positives RT-PCR results with Ct value≤35 for the COVID-19 genes at IEDCR will be selected as COVID-19 positive. Samples with negative RT-PCR results with no amplification for the COVID-19 genes at IEDCR will be selected as COVID-19.  Exclusion Criteria:  Samples with equivocal/ambiguous RT-PCR results in terms of sigmoidal curve and Ct value will be excluded.