Inclusion Criteria:  systemically healthy and non-smoker individuals having ≥20 teeth present (except third molars) individuals with periodontally healthy, periodontitis stage III grade B and C diagnoses  Exclusion Criteria:  having any diagnosed medical disorders such as diabetes mellitus, cardiovascular diseases, rheumatoid arthritis, immunological and mucocutaneous diseases usage of antibiotics, non-steroidal anti-inflammatory drugs and immunosuppressive agents within the past 6 months having any non-inflammatory destructive periodontal disease nonsurgical/surgical periodontal therapy received in the past year pregnant/ lactating/ postmenopausal females.