Inclusion Criteria:  Patient aged 35-65 years old Patient with knee pain that had been continuing for at least 12 months with no relief using anti-inﬂammatory medications and that deteriorated with weight-bearing Knee osteoarthritis that classified as mild-moderate or moderate-severe (Kellgren-Lawrence Grade 2, 3, or 4)  Exclusion Criteria:  Age > 65 years The recent history of knee trauma Autoimmune rheumatic diseases Accompanying severe hip OA Thrombocytopenia or other blood diseases Immunosuppressive or anticoagulant treatment The invasive procedure applied to the knee Intra-articular steroid injection to the knee within the previous 12 months Previous joint infection Uncontrolled systemic diseases such as diabetes or hypertension, and cancer Excessive varus/valgus knee deformity