Inclusion Criteria:  Age (16-70 years), male and female; HIV-1 infected; hospitalized patients with the signs or symptoms inferred to infection; Initial CD4 cell count less than 200 cells/ul; HIV-1 RNA viral load was unlimited; State Informed Consent for Free Treatment has been signed; Good compliance and signing Informed Consent  Exclusion Criteria:  have been treated regularly in other hospitals or have been clearly diagnosed before admission; Researchers decide if patients could/not complete the scheduled follow-up (factors to consider such as weak, poor compliance, etc.); Pregnancy during the study period; Hospital stay less than 7 days.