Inclusion Criteria:  Able to provide informed consent Age between 18 to 85 Body Mass Index (BMI) between 25 and 40 kg/m2 Individuals with normal glucose levels, pre-diabetes and diabetes Type 2 for at least 1 year (diagnosed by ADA criteria) and up to 20 years Stable dose of meds for 3 months Stable diet and lifestyle for 3 months Medical history without clinically significant abnormalities  Exclusion Criteria:  Have a disorder that would impede performing the HRV measurement procedure (i.e., abnormal cardiac rhythm, heart disease, including coronary artery disease, angina, and arrhythmia, cardiac pacemaker, stroke, panic attack, cognitive impairment, renal failure) Chronic disease (e.g. HIV, Cushing syndrome, CKD, acromegaly, active hyperthyroidism etc.) Cancer and anticancer treatment in the last 5 years Pregnancy or lactation Subjects with major physical disability Subjects with previous history of cerebrovascular accident Any disorder, which in the investigator's opinion might jeo