Inclusion Criteria:  Patients with stage IIIB-IV NSCLC in AJCC TNM stage (8th edition) confirmed with histology or cytology; Newly diagnosed; There are sufficient samples that meet the requirements for examination; Eligible for the next anti-tumor systemic treatment; Willing to sign the consent inform, age ≥18 years; Patients willing to cooperate with the planned follow-up schedule; Permit to collect clinical data needed by the institute.  Exclusion Criteria:  Diagnosed with other malignant tumors at the same time (except for fully treated cervical carcinoma in situ, basal or squamous cell skin cancer); The patient has other serious diseases that may affect follow-up and short-term survival; Any other medical conditions and social/psychological problems that investigator evaluated is not suitable to enroll in this study; Absents of contrast-enhanced magnetic resonance imaging (MRI) or contrast-enhanced computer tomography (CT) during clinical follow-up.