Inclusion Criteria:  Patients with stage I-IV non-small cell lung cancer(with no restriction of age, gender, or smoking history) Patients in the group will be allowed to collect whole blood and tissue samples at specific time points Eastern Cooperative Oncology Group Performance Status of 0-1 within 28 days prior to registration No previous systemic anti-tumor therapy Signed informed consent  Exclusion Criteria:  Active or history of autoimmune disease or immune deficiency Patients with serious mental disease Prior allogeneic stem cell or solid organ transplantation Pregnant or lactating women Patients who cannot obtain tumor tissue samples and / or whole blood Patients with history of blood transfusion within half a year Patients with any other malignancy diagnosed within 5 years Received systemic anti-tumor therapy