Inclusion Criteria:  Female between 18 to 70 years old. Patients diagnosed as invasive breast cancer by pathology. Clinical stage considered as T1-4N0-3M0. HER2 overexpression by IHC or FISH positive. Patients plan to accept neoadjuvant therapy, or plane to accept adjuvant therapy after neoadjuvant therapy and surgery.  Exclusion Criteria:  Patients have already accepted any other anti-tumor treatment not included in our project. Patients with metastasis or not considered surgery therapy. Patients cannot accept chemotherapy or molecular targeted therapy, because of severe cardiovascular diseases or other reasons