Inclusion Criteria:  Provide informed consent prior to initiation of any trial procedures. Be able to understand and agrees to comply with planned trial procedures and be available for all trial visits. Agrees to the collection of venous blood per protocol. Healthy male or non-pregnant female, between 18 and 75 years of age (Group 1 and Group 2 = 18 to 55 years cohort; Group 3 and Group 4 = 56 to 75 years cohort), inclusive at time of enrollment who are at lower risk of SARS-CoV-2 infection, defined as adults whose health status, profession, locations or circumstances put them at lower risk of exposure to SARS-CoV-2 and COVID-19. Body Mass Index (BMI) <40.0 kg/m2 (or < 35.0 kg/m2 if obesity-related health conditions are present) at screening. Women of childbearing potential* must agree to use or have practiced true abstinence** or use at least one acceptable primary form of contraception.***, **** Note: These criteria are applicable to females in a heterosexual relationship and child-bearing potential (i.e., the criteria do not apply to subjects in a same sex relationship). Women of childbearing potential must have a negative urine or serum pregnancy test within 24 hours prior to vaccination. Male subjects of childbearing potential*: use of condoms to ensure effective contraception with a female partner of childbearing potential from vaccination until 90 days after vaccination. If barrier methods are to be used, then double barrier methods of protection are required i.e. male condom with a cap, diaphragm or sponge with spermicide. Male subjects agree to refrain from sperm donation from the time of vaccination until 90 days after vaccination. Female subjects agree to refrain from egg donation from time of vaccination until 90 days after vaccination. In good health. Oral temperature of 97.0°F (36.1°C) to less than 100.4° Fahrenheit (37.8° C). Pulse is less than 100 beats per minute. Systolic blood pressure (BP) is 85 to 150 mm Hg, inclusive. Diastolic blood pressure <90 mmHg, inclusive. Repeat blood pressure measurements are permitted. Clinical screening laboratory evaluations (white blood cells (WBC) [total and differential counts], hemoglobin, platelets, alanine transaminase, aspartate transaminase, creatinine, alkaline phosphatase, total bilirubin, lipase, prothrombin time and partial thromboplastin time) are within acceptable normal reference ranges at the clinical laboratory being used. Alternatively, the clinical laboratory abnormalities grading scale noted in the FDA Toxicity Grading Scale for Healthy Adult Volunteers enrolled in Preventive Vaccine Clinical Trials may be used. Must agree to have samples stored for secondary research. Agrees to adhere to pandemic public health guidance on preventing SARS-CoV-2 infection (e.g. wearing a mask, keeping physically distant, sheltering-in) throughout trial duration. Must agree to refrain from donating blood or plasma during the trial (outside of this trial). Seronegative to SARS-CoV-2 and negative for SARS-CoV-2 by PCR.  Exclusion Criteria:  Positive pregnancy test either at screening or just prior to vaccine administration. Female subject who is breastfeeding or plans to breastfeed from the time of the vaccination through 60 days after vaccination. Anyone at high risk of severe COVID-19 disease e.g. those with hypertension, hyperlipidemia, diabetes mellitus, types 1 and 2, chronic lung disease, etc… per CDC guidance (< https://www.cdc.gov/coronavirus/2019-ncov/hcp/clinical-care/underlyingconditions.html>) or has any medical disease or condition that, in the opinion of the participating site PI or appropriate sub-investigator, precludes trial participation.* Presence of self-reported or medically documented significant medical or psychiatric condition(s). Has an acute illness, as determined by the participating site PI or appropriate sub-investigator, with or without fever [oral temperature ≥ 38.0° Celsius (100.4° Fahrenheit)] within 72 hours of vaccination. Has a positive test result for hepatitis B surface antigen, hepatitis C virus antibody, or human immunodeficiency virus (HIV) types 1 or 2 antibodies at screening. Has participated in another investigational trial involving any investigational product (unlicensed vaccine, drug, biologic, device, blood product, or medication) within 60 days, or 5 half-lives, whichever is longer, before vaccine administration. Currently enrolled in or plans to participate in another clinical trial with an investigational product (unlicensed vaccine, drug, biologic, device, blood product, or medication) that will be received during the trial-reporting period. Has previously participated in an investigational trial of prophylaxis or treatment for SARS-CoV-2 infection or COVID-19 disease. Has a history of hypersensitivity or severe allergic reaction (e.g., anaphylaxis, generalized urticaria, angioedema, other significant reaction) to any previous licensed or unlicensed vaccines. Chronic use (more than 14 continuous days) of any medications that may be associated with impaired immune responsiveness Anticipating the need for immunosuppressive treatment within the next 6 months. Received immunoglobulins and/or any blood or blood products within the 4 months before vaccine administration or at any time during the trial. Has any blood dyscrasias or significant disorder of coagulation. Has any chronic liver disease, including fatty liver. Has a history of alcohol abuse or other recreational drug (excluding cannabis) use within 6 months before vaccine administration. Received or plans to receive a licensed, live vaccine within 4 weeks before or after vaccination. Received or plans to receive a licensed, inactivated vaccine within 2 weeks before or after vaccination. Receipt or planned receipt during the study period of a COVID-19 vaccine. Close contact of anyone known to have SARS-CoV-2 infection within 30 days prior to vaccine administration. History of COVID-19 diagnosis (positive test for antigen or PCR or antibody). On current treatment with investigational agents for prophylaxis of COVID-19. Plan to travel outside the United States (US) (continental US, Hawaii, and Alaska) from enrollment through 28 days after vaccination. Reside in a nursing home or other skilled nursing facility or have a requirement for skilled nursing care. Non-ambulatory. For subjects of any age, individuals currently working with high risk of exposure to SARS-CoV-2.