Inclusion Criteria:  - 1.Healthy group were as follows: no history of hepatitis, no history of liver cirrhosis, no history of liver cancer and receiving the whole-course COVID-19 vaccination.  2. Overweight people are defined as having a BMI of more than 24 and receiving the whole-course COVID-19 vaccination.  Exclusion Criteria:  Patients previously diagnosed or with a history of contact with confirmed cases