Inclusion Criteria:  Age must be 18 years or older Able to fast overnight Can maintain diet, medications, and sleep habits between each of the testing visits. Lifestyle is consistent with participation in a study evaluating reductions of inactivity (defined by sitting at a low metabolic rate because of minimal muscular activity) Willing and able to wear small wearable activity monitors under their clothes as instructed. No plans to start a new diet or exercise program if enrolled in the present study.  Exclusion Criteria:  Practical barriers to completing the study, such as plans to move, or work, or family commitments. Plans to change lifestyle during the present study. Have physical, vocational, or reasons that prohibit ordinary sitting behaviors present in the modern world. Currently dieting to change body weight, or have eating disorder. Are pregnant or plan to become pregnant. Taking medications that affect bleeding (ie anticoagulants). Allergy to lidocaine excludes biopsy component of study.