Inclusion Criteria:  Each subject must meet all the inclusion criteria to be eligible for the study.  Subjects or their legal representatives are able to sign an informed consent form Subjects are committed to comply with the study procedures and cooperate with the implementation of the full study Subjects are 18 years of age or older, regardless of gender Subjects with high suspicion of HCC, or diagnosed HCC without tumour-related treatment (supporting evidence including serum AFP, MRI, CT and other imaging data and histopathology), and in good general condition Subjects intend to obtain pathological results by biopsy or surgical resection  Exclusion Criteria:  All patients meeting any of the exclusion criteria will be excluded from the study.  Patients or their legal representatives are unable or unwilling to sign the informed consent form Patients are unable to cooperate in the conduct of the full study Patients with acute systemic illness and electrolyte disturbances Patients with other previous malignancies or a combination of other malignancies Patients who are pregnant or lactating women