Inclusion Criteria:  The subject ages ranges from 20-45 years old. The subject has no chronic pain symptoms or complaint in last 6 months. The subject is subjectively able to discriminate sng and pain. The subject has no history of major diseases that required treatment or currently being under treatment. Gender: men and women half The used hand of subject is the right hand. The educational level of subject is more than 9 years (graduated from junior high school) The subject didn't have physical and mental illness The subject didn't take prescribed medicine. The VAS questionnaire must be 0 point both of low back "pain" and low back "soreness" assessment. The subject who can fill the informed consent after understanding the purpose and medical help of this trial.  Exclusion Criteria:  The subject has:Neuropathic pain due to causes other than that specified in the inclusion criteria (e.g., post-herpetic neuralgia; painful diabetic neuropathy; mononeuritis multiplex; central poststroke pain; failed back surgery in relation to the presenting episode of radiculopathy; spinal abscess, infection, hematoma, or malignancy; phantom limb pain; peripheral neuropathy due to alcoholism, malignancy, human immunodeficiency virus [HIV], syphilis; drug abuse; vitamin B12 deficiency; hypothyroidism; liver disease; toxic exposure).  Pain that is associated with a substantial somatic pain component (e.g., non-neuropathic/musculoskeletal pain in lower limbs or other parts of the body apart from the back) or more than one cause or potential cause for pain symptoms.Any painful concurrent rheumatic disease such as, but not limited to, fibromyalgia, rheumatoid arthritis, or significant osteoarthritis.  The subject is unable to reliably delineate or assess his or her own pain by anatomical location/distribution (e.g., the subject cannot reliably tell the difference between his or her back pain and lower limb pain and cannot rate the intensity of each separately). The subject has undergone lumbar spine surgery within the last 6 months or has received treatment with epidural injections, nerve blocks, or acupuncture for LSR within 4 weeks before screening. The subject had a malignancy according to his/her report. The subject has had a positive test for HIV antibody or a history of HIV according to his/her report. The subject has had a positive test for hepatitis B surface antigen or hepatitis C antibody according to his/her report. The subject has a history of alcohol or narcotic substance abuse according to his/her report. The subject is female and is pregnant or breastfeeding at the time of the screening visit or plans to become pregnant during the study period. The subject cannot perform brain MRI scanning who had metal implants of head (such as fixed dentures, metal bone plate, vascular clamp, vascular embolization treatment coil, deep brain stimulator, artificial electronic ear, etc.), implants of head which affecting the image quality (such as the ventricle peritoneal catheter, etc.), implantation of permanent heart rate regulator, etc. The subject has a history of spinal surgery. The VAS questionnaire not be 0 point either low back "pain" or low back "soreness" assessment. The subject has suffered from claustrophobia. The subject has suffered from brain disease and had brain surgery. The subject has taken prescribed medicine which can affect specific function of brian (such as sleeping pills, tranquilizer, etc.). The subject has mental comorbidity (such as depression, panic disorder, etc) The subject has mental retardation. The educational level of subject is less than 9 years. The subject ,who is unable to understand the purpose of this trial and fill the informed consent.