Inclusion Criteria:  acquired brain injuries less than 1 year and more than 28 days in DOC; clinical diagnosis of DOC Disease; no medical history of neuropsychiatric diseases; no contraindications for rTMS or EEG, no sedatives in use or other drugs that might interfere with brain stimulation, such as Na+ or Ca2+ channel blockers or NMDA receptor antagonists; stable state of disease and vital signs; the families of the patients volunteered the patient to participate in the study and provided signed informed consent; the integrity of the individualized stimulation target cortex are verified by MRI.  Exclusion Criteria:  patients in other non-invasive or invasive neuroregulation trials; motor evoked potential (MEP) in M1 region cannot be induced by TMS pulse; uncontrolled epilepsy, seizure within 4 weeks before enrollment; metallic implant in the skull, pacemaker, craniotomy under the stimulated site, implanted brain device.