Inclusion Criteria:  . Preterm infants born between 23 weeks and 0 days and 30 weeks and 0 days gestation.  Exclusion Criteria:  Congenital anomalies i.e any major antenatal diagnosed congenital abnormalities such as congenital heart disease, suspected or known chromosomal abnormalities. Infants not born at UCLH where baseline data and samples cannot be collected