Inclusion Criteria:  Bladder cancer group:  Any male or female patient aged 18 or older Able to provide serum and urine specimen before treatment Diagnosis with incident or recurrent bladder cancer  Control group  Any male or female patient aged 18 or older Able to provide serum and urine specimen before treatment Diagnosis with benign disease of the urinary system, such as urinary calculi and benign prostatic hyperplasia.  Exclusion Criteria:  Kidney and liver dysfunction. Incomplete clinical information and laboratory test result Tumor-related treatment was received preoperatively Preoperative routine examination and medical history indicate a history of diabetes, hyperlipidemia and other metabolic diseases or other malignant tumors