Inclusion Criteria:  Patients who have undergone microneurosurgical resection of the tumor in our hospital and are pathologically confirmed to be supratentorial glioma; Preserving the remaining tissue samples of paired gliomas and PTBE for pathological examination; The patients agreed to be enrolled and signed the informed consent; The clinical data of the patients are complete and not lost; No serious complications occurred after surgery.  Exclusion Criteria:  All glioma patients who did not meet the inclusion criteria