Inclusion Criteria:  Patients voluntarily participate the study and sign informed consent, with good compliance and follow-up; Patients who can tolerate chemotherapy and targeted therapy, ECOG≤1 point; Patients with advanced colorectal cancer diagnosed by pathology and imaging; 4. Neutrophil count ≥2.0×10^9/L, Platelet count ≥100×10^9/L, Hemoglobin ≥90g/L, Serum total bilirubin ≤ 1.5 times the upper limit of normal value, Alanine aminotransferase and Aspartate aminotransferase ≤ 1.5 times the upper limit of normal value, and Serum creatinine ≤ 1.5 times the upper limit of normal value.  Exclusion Criteria:  Patients who cannot obtain enough tissue for gene test and the organoids-on-a-chip culture; Patients previously combined with other malignant tumors of other organs; Patients who need emergency operation due to perforation and obstruction； Patients who have active hepatitis, HIV (+) and other conditions that cannot tolerate chemotherapy; Patients who have serious heart and lung diseases, vascular diseases, mental diseases or drug abuse.