Inclusion Criteria:  participants without allergy-related diseases, non-pregnant women and who agreed to cooperate in carrying out the research by signing the consent form aged between 18 and 60 years of both sexes.  Exclusion Criteria:  participants who had a history of previous anaphylaxis, extensive dermatitis, use of antihistamines, colds or steroids. participants who had a negative reaction to histamine and/or withdrew their consent at any stage of the study.