Inclusion Criteria:  Confirmed breast cancer any stage confirmed by radiological and pathological or by clinical evaluation undergo surgery or receiving neo/adjuvant chemotherapy treatment Age above 18 years HER2 negative core biopsy Adequate renal function: the calculated creatinine clearance should be ≥50 mL/min Patients must be accessible for treatment and follow-up Performance status of Eastern Cooperative Oncology Group (ECOG) ≤ 2  Exclusion Criteria:  Known hypersensitivity reaction to the investigational compounds or incorporated substances patients who are unlikely to comply with trial requirements (eg, confusion, psychological or mood disturbances ,alcoholism, cardiac arrhythmia)