Inclusion Criteria:  The primary school children who are studying in class 1 to class 5 among male, female or others gender. The children's parents or legal guardians would like to give consent and are willing to participate in the study. Those, who will be agreed to receive health check-ups, answering questionnaire and are willing to give the sample for laboratory investigation. Who will stay in the same school and area till study completion.  Exclusion Criteria:  The child is absent from school during the health checkup periods (at baseline) due to severe illness or without any reason and information. Who does not want to give consent and is not willing to participate. Who does not want to share their information and does not want to give the sample for laboratory investigations.