Inclusion Criteria:  60 male or female patients with a newly diagnosed laryngeal squamous cell carcinoma Patients without any previous history of laryngeal carcinoma 20 healthy, age- and sex- matched controls  Exclusion Criteria:  Previous history of laryngeal squamous cell carcinoma Having comorbid systemic diseases like DM, hypertension, cardiologic or rheumatologic diseases Having malign tumors elsewhere History of chemotherapy/radiotherapy.