Inclusion Criteria:  Pathologically confirmed diagnosis of esophageal squamous cell carcinoma； Immunotherapy for preoperative； Karnofsky performance status 0-1; The postoperative pathological stage is (y) Pt + and / or N + M0 according to AJCC 8th Function of the main organs is normal; Edition Patient's written informed consent  Exclusion Criteria:  Tumor emergencies; Abnormal coagulation function; Contagious diseases, such as HIV, HBV, HCV infection; Mental disorders; Concomitant tumors; Immunological co-morbidities