Inclusion Criteria:  Trial group:  Research period: March 9, 2021 to March 9, 2022; Research site: The First Affiliated Hospital of Shandong First Medical University; Healthy female college students with normal menstruation of Shandong University of Traditional Chinese Medicine; Menstrual period in the same menstrual cycle: Days 2nd-4th of the menstrual cycle; Unmarried female college students aged 18-27 years old; Female college students with informed consent to accept this pulse collection.  Control group:  Research period: March 9, 2021 to March 9, 2022; Research site: The First Affiliated Hospital of Shandong First Medical University; Healthy female college students with normal menstruation of Shandong University of Traditional Chinese Medicine; Non-menstrual periods of the same menstrual cycle: Days 20th-28th of the menstrual cycle; Unmarried female college students aged 18-27 years old; Female college students with informed consent to accept this pulse collection.  Exclusion Criteria:  Special menstruation: bimonthly menstruation, seasonal menstruation, annual menstruation and latent menstruation; Pregnant or lactating women; Ovulation period: generally on the 14th day before menstruation and three days before it and four days after it; Those with dysmenorrhea; Those with severe diseases; Patients with uterine or adjacent organ tissue diseases, including gynecological diseases, and diseases of waist, pelvis, colon and urinary system； Those with infectious diseases; Those who are not easy to collect pulse manifestation such as pulse on back of wrist and oblique-running pulse; Incomplete information that affects the judgment of results due to subjective or objective reasons; Those with tremor of limbs.