Inclusion Criteria:  Study group: Untreated women diagnosed with PCOS that are planned for intervention.  Control group: Women without PCOS visiting the gynecologic outpatient's clinics.  Exclusion Criteria:  Endocrine abnormality ( Cushing's syndrome, congenital adrenal hyperplasia, thyroid disorder, hyperprolactinemia and androgen-secreting tumor). Premature ovarian failure 2. Active malignancy 3. Participants taking antibiotics/ probiotics, hormonal, vaginal or laxative medicine (in the previous week).  4. Vaginitis/ Pelvic Inflammatory Disease (PID)