Inclusion Criteria:  Inclusion Criteria  Subjects 18 years of age or older, all sexes and races Willing to sign a written informed consent to participate Corneal ulcer criteria: at least 3mm in diameter, opacification located within 3mm of visual axis, infiltrate occupying at least 50% of the corneal thickness, moderate AC cell reaction, clinical picture consistent with bacterial infection later confirmed by culture and gram stain.  Exclusion Criteria:  History of Immunodeficiency History of connective tissue disorders or severe atopic disease History of chemical eye injuries History of known limbal stem cell deficiency History of neurotrophic keratopathy History of recent eye surgery, or glaucoma surgery with bleb or drainage tube Risk factors and clinical appearance consistent with fungal keratitis