Inclusion Criteria:  Males (20-60 years old ) and premenopausal females with NAFLD diagnosed by Fibroscan  Exclusion Criteria:  (i) Patients aged <20 years or >60years. (ii) Alcohol consumption of ≥30 g/day in men or≥20 g/day in women. (iii) Subjects with suspected or proven any other liver disease other than NAFLD as (viral hepatitis, drug-induced liver injury, autoimmune liver disease, Wilson's disease or primary biliary cholangitis).  (iv) Postmenopausal females to avoid the increased risk of osteoporosis in those patients due to hormonal changes.  (v) Patients with conditions known to affect bone metabolism, such as kidney, thyroid or parathyroid diseases.