Inclusion Criteria:  - Man  Male infertility (primary or secondary infertility) Age 22 ~ 80 years old; Body mass index < 30 kg / m2; Normal levels of FSH, LH, t and PRL; Semen concentration and vitality were normal or slightly abnormal (TSC ≥ 10 million / ml, PR ≥ 20%, vitality ≥ 30%), which was consistent with the indication of IUI assisted pregnancy; On the day of IUI, the male side took the semen and the TSC was less than 10m / ml  Women's standard  Age 20 ~ 40 years old Body mass index < 30 kg / m2;  3）FSH<10U/L、AMH>1.1ng/ml  4) Bilateral fallopian tubes are unobstructed  Exclusion Criteria:  Man  It has performed vasectomy, orchitis, unilateral orchiectomy, ejaculation difficulty, retrograde ejaculation, diabetes, cardiovascular disease, drug treatment of psychological or genetic diseases.  Woman  Previous history of intrauterine adhesion; Hydrosalpinx; B-ultrasound suggests that endometriosis cyst may be;