Inclusion Criteria:  women that have regular menses (menstrual cycle length 21-35 days) women who will come visits regularly women that are trying to conceive more than 1 year if women age is less than 35 years or more than 6 months if women age is >35 years  Exclusion Criteria:  women who do not menstruate regularly women that use hormonal contraception women who take drugs that will interfere with steroid hormone metabolism