Inclusion Criteria:  1. All participants must have HLHS (includes all types) requiring Stage II palliation (Glenn or Hemi-Fontan operation).  Exclusion Criteria:  Requirement for ongoing mechanical circulatory support immediately prior to Stage II palliation within 5 days Need for concomitant surgery for aortic coarctation or tricuspid valve repair Undergoing the Stage I (Norwood) procedure that does not have HLHS Serum positivity for: HIV; hepatitis B virus surface antigen (HBV BsAg); and/or viremic hepatitis C virus (HCV). If test results are not available, blood will be obtained during screening window to check for positivity Parent/guardian that is unwilling or unable to comply with necessary follow-up Unsuitability for the study based on the Investigator's clinical opinion Known hypersensitivity to dimethyl sulfoxide (DMSO)