Inclusion Criteria:  male gender aged between 18 and 40 years who practice soccer for more than 6 hours / week from the age of 10 to the present.  Exclusion Criteria:  play other elite sports than soccer play 6 or more hours of recreational sports per week smokers individuals with chronic cardiovascular diseases, hypo and hyperthyroidism, hypertension, rheumatoid arthritis and osteoarthritis