Inclusion Criteria;  Clinical diagnosis of Migraine Clinical diagnosis of Tension- type headache Age between 18 and 65 years Body mass index between 18 kg/m2 and 30 kg/m2  Exclusion Criteria;  Secondary headaches Chronic inflammatory disorders Infectious diseases Hematologic abnormalities A prior history of surgery (within the preceding 6 months) Receiving analgesics Blood transfusion before admission