Inclusion Criteria:  Healthy men and women 18-35 years of age Healthy men and women 65-85 years of age  Exclusion Criteria:  Serious acute/chronic illness (e.g., active cancer, inflammatory states, RA, SLE, or a CVD event within past 6 months) Diabetes or pre-diabetes with an A1c >6.0% Pregnancy BMI >30 kg/m2 or <20 kg/m2 eGFR <45 ml/min ALT >3x ULN Hct <35 or Hb <10 Exclusionary meds: calcium channel blockers, anticonvulsants or other drugs shown to affect autophagy (see table below) Food allergy or known food intolerance Active Smoking (>1 cigarette or cigar per week) Use of recreational drugs (opioids, cocaine, marijuana, etc.) in past month Use of alcohol on the day prior to and the day of study Shift workers or other dysregulated sleep pattern (habitual use of sleep medications, jet lag, etc.) Strenuous exercise within 3 days prior to study visit 2 Any condition the investigator believes would impair the ability to interpret targeted outcomes