Inclusion Criteria:  All participants must fulfill the following inclusion criteria: -  fulfillment of either of these five categories of criteria, depending on how many clinical attacks have occurred patients aged 18 to 35 years of age. Males and females are included. All patients who received diagnosis of multiple sclerosis whether under treatment or not.  Exclusion Criteria:  1) Patients age of below 18 years or over 35 years . 2) History suggestive or diagnosed collagen disease such as Bechet disease. 3) History suggestive with other general medical disease