Inclusion Criteria:  CKD stage 3-5 (eGFR < 60 mL/min/1.73m3) CKD stage 5 patients receiving HD,CAPD and KT for more than 3 months. The participants of the healthy control group consists of volunteer health care workers who had been vaccinated according to the nation vaccination schedule  Exclusion Criteria:  allergy to the components of vaccines unable to receive the vaccine according to the schedule fever or concomitant serious illnesses had side effect from the first dose of vaccination prior COVID-19 infection in the past (diagnosed via patients history and serological test for nucleocapsid (NCP) antibody