Inclusion Criteria:  Age ranged from 18 to 30 years, from both sex. Healthy general medical condition, healthy periodontal condition. Malocclusion that requires extraction of the maxillary first premolars, followed by canine retraction, with moderate lower incisor crowding (Little's irregularity index 3-6mm). Normal shape and structure of maxillary canine, with no history of filling or root canal treatment and normal shape and structure of maxillary first molar.  Exclusion Criteria:  Pregnant women. Patient with renal or liver disease. Patient taking corticosteroid or anticonvulsant drugs. Patient with thyroid or parathyroid problems.