Inclusion Criteria:  Obesity : BMI>=30kg/m2,or percent body fat(PBF) >=25% for male, >=30% for female.  （Note: If PBF was eligible, it was recommended that 26 ≤ BMI ≤ 40）  Age: 40 - 65 years old adults Who has the conditions to preserve the test samples at low temperature throughout the whole process  Exclusion Criteria:  Patients with severe chronic diseases（heart, liver, kidney, hematopoietic system, tumor and other serious diseases, mental illness and organ surgery history, etc.) and complications; Patients with severe allergy and immunodeficiency; Patients with a clear history of gastrointestinal diseases (ulcer, irritable bowel syndrome, etc.); History of intervention with fat-reducing drugs or health products in the past 2 months Take weight control measures (diet, exercise, etc.) within the past month Participation in other clinical trials within the past 3 months who have used antibiotics in the past 2weeks ; Those who cannot guarantee to maintain their current lifestyle during the trial period Those who fail to consume the tested samples as required, or fail to follow up on time, resulting in failure to determine the efficacy