Inclusion Criteria:  Men and women between the ages of 18 and 29 who are in good health. BMI ranging from 18.5 to 22.9 kg/m2 (classification of IDI & WPRO for Asians). Voluntarily consented to participate in the study, read, was thoroughly explained, and signed the consent form to participate in the study. Mental and physical well-being were assessed throughout the study period (on the Depression Anxiety and Stress Scale of 21 with a stress score of 15 points). Vital signs are within normal ranges:  Pulse: 60-100 pulses per minute Systolic blood pressure: 90 to 139 mmHg. Diastolic blood pressure: 60 to 89 mmHg. Temperature: 36.3-37.1 degrees Celsius. At rest, the breathing rate is 16 3 times per minute, and the SpO2 level is 92%.  - Volunteers have no other chronic medical conditions: Thyroid disease, autonomic neuropathy, hypertension, diabetes, respiratory diseases (asthma, pneumonia, chronic obstructive pulmonary disease, etc.) etc) by asking for medical history and medical history.  Exclusion Criteria:  A feeling of fear or anxiety appeared immediately before the test (rated on the Depression Anxiety and Stress Scale of 21 with stress scores greater than or equal to 15 points). Use of stimulants such as alcohol, beer, coffee, and tobacco within 24 hours before the study. The auricular skin was examined with lesions (scars, lacerations, scratches, bites).