Inclusion Criteria:  18 and 65 year-old patients. Patients with pre-treated teeth diagnosed with symptomatic or asymptomatic apical periodontitis. Patients who can tolerate the treatment physically and mentally.  Exclusion Criteria:  The presence of any systemic disease, the presence of any allergic reactions or pregnancy, use of any analgesic or antibiotic medication within 7 days, use of corticosteroid within 6 months, severe periodontal disease (periodontal pockets >3 mm) in the pretreated tooth, surgical endodontic treatment, having drainage, periapical index (PAI) score 1 and 2, excessively curved, excessively long or short roots, calcified or resorbed root canals, immature apices, complications like perforation, overfilling, broken files