Inclusion Criteria:  Male/ female at all age- newborn to adult. Non- pregnant woman. Diagnose of middle ear infection and plan for surgery procedure.  Exclusion Criteria:  Pregnant woman Incompetent adults (i.e. individuals with cognitive impairment) No diagnose of middle ear infection and no plan for surgery procedure. Adults with cognitive impairment and children of parents that are cognitively impaired and thus are unable to consent will be excluded from the study.