Inclusion Criteria:  . Age 18-70 years old; . A history of chronic liver disease, chronic acute liver failure (ACLF) caused by various causes, 1-2 organ failure (ACLF diagnosis is based on the European EASL diagnostic criteria, and the organ failure criteria is based on CLIF-OF); . The patient or adult family members agree to participate in this study and sign an informed consent form;  Exclusion Criteria:  Those who meet any of the following conditions will not be included:  Those who intend to undergo artificial liver treatment; Those who have received liver transplantation and any form of stem cell therapy; Malignant tumors in or outside the liver; or imaging findings suggest malignant mass in the liver or tuberculosis; Complicated with severe autoimmune diseases; combined with severe cardiopulmonary insufficiency; renal replacement therapy; Immunosuppressive therapy; HIV/tuberculosis infection; alcohol/drug addiction; participated in drug trials in the past 3 months; Gastrointestinal bleeding or serious infection occurred in the past one month; Pregnant and lactating women; Those who do not have the ability to make independent judgments and have no direct adult family members to sign the informed consent form.