Inclusion Criteria:  Patients with confirmed (i) urinary tract infection, (ii) pneumonia (including patients after lung transplantation, cystic fibrosis) or (iii) deep-seated infection with focus pathogen:  E. coli Klebsiella species S. aureus P. aeruginosa Controls: no detectable bacteria in routine microbiology lab and no other infection site at inclusion of the sample and follow up for 10 days, signed general consent  Clinical controls without obtained samples, but with confirmed (i) urinary tract infection, (ii) pneumonia (including patients after lung transplantation, cystic fibrosis) or (iii) deep-seated infection with focus pathogen:  E. coli Klebsiella species S. aureus P. aeruginosa  Exclusion Criteria:  Patients who have refused research and reuse of their data/samples (e.g. general consent) or any other decline (e.g. Patientenverfügung). other than one of the focus bacteria in routine microbiology lab Age: <18 years Controls without signed general consent