Inclusion Criteria  Ages 20 and above. Pathological reported showed adenocarcinoma、squamous cell carcinoma、large cell carcinoma、adenosquamous carcinoma, and sacromatoid carcinoma. For patients with squamous cell carcinoma, only never smokers and light smokers (less than 10 cigarettes per day) are indicated. For patients with advanced EGFR/ALK (-) tumors, treatment-naïve or failure to ≤ two lines of systemic treatment. For patients with advanced EGFR/ALK (+) tumors, failure to ≤ two lines of systemic treatment, such as tyrosine kinase inhibitors. Reacquisition of tumor tissue after the failure of previous systemic treatment Willingness to provide the residual biopsy/operative slides. Life expectancy more than 3 months. Patients fully understand the protocol with the willingness to have regular follow-up.  Exclusion Criteria  Inability to cooperate by providing a complete medical history. No available tumor tissues for genetic testing. Undesirable compliance.