Inclusion Criteria:  Symptoms suggestive of TB disease: cough for more than two weeks plus at least one of the following: fever, malaise, weight loss, night sweats, haemoptysis, chest pain or loss of appetite. Willingness to give consent to take part in the study. Aged >18 years old. Healthy control : no history of TB treatment, no respiratory symptoms, no evidence of active infectious disease, no history of close contacts of active pulmonary TB patients.  Exclusion Criteria:  Pregnant woman A person who has received treatment for active TB or LTBI in the past 24 months