Inclusion Criteria:  patient  Age is greater than or equal to 18 years old (including 18 years old); Patients who are diagnosed with cancer after pathological diagnosis; Can provide enough tissue samples; Have not received anti-tumor treatment in the past; Comply with the principle of informed consent and sign the informed consent form.  healthy person:  Age is greater than or equal to 18 years old (including 18 years old); No underlying disease; Can provide enough tissue samples; Comply with the principle of informed consent and sign the informed consent form.  Exclusion Criteria:  The tumor foci cannot be clearly derived from a specific tumor type; Those who have a history of other tumors, autoimmune diseases and allergies; Previously received anti-tumor treatments such as surgery, chemotherapy, radiotherapy, molecular targeted therapy, immunotherapy, biological therapy or Chinese medicines with anti-cancer and anti-tumor effects in the instructions; Participated in any clinical trial within 90 days before the experiment; Received major surgery within 90 days before the experiment; Received bone marrow transplantation 90 days before the experiment; Subjects who may not be able to complete the study due to other reasons or who are determined by the investigator to be unsuitable to participate in this study.