Inclusion Criteria:  Aged 18 years or older;  Meeting the eligibility criteria of the NPP i.e. being a MSM or transgender persons who in the 6 months prior to the PrEP-request/PrEP-consultation:  Had anal sexual intercourse without a condom with a male partner with an un-known HIV status, and/or; Had anal sexual intercourse without a condom with a male partner with a known HIV-positive status and a detectable viral load, and/or; Was diagnosed with a rectal STI, and/or; Received a prescription for post-exposure prophylaxes (PEP); Living in the catchment area of one of the participating GGD regions; Having a smartphone, internet access and email address; Sufficient understanding of Dutch or English; and Signed informed consent.  Exclusion Criteria:  HIV infection; Chronic or acute Hepatitis B virus infection; Diminishing renal function: eGFR less than 60 mL/min/1.73m2; Other renal problems, as diagnosed by a physician and advised against using TFV; Chronic use of medication that interacts with TFV/FTC e.g. NSAIDs. Other medical conditions that require special attention when using PrEP, such as osteoporosis or other bone diseases; Unlikely, in the opinion of the clinician, to comply with the study requirements or procedures; Participating in another study that affects the primary or secondary outcome measures of our study; Investigators or otherwise dependent persons.