Inclusion Criteria:  Patients included in the study were healthy males and females (Category: American Society of Anesthesiologists class 1) aged 21-49 years with no physical disability, facial paresthesia or psychological problems presenting with a single vital mature mandibular first molar, with signs and/or symptoms of symptomatic irreversible pulpitis  Exclusion Criteria:  Pregnant women patients who took analgesics or other medications that would alter the inflammatory response of the pulp or provide analgesia 6- hours pre-operatively those with known sensitivity to the pharmaceuticals used in this trial. those with pathological periodontal pockets