Inclusion Criteria:  In order to be eligible to participate in this study, an individual must meet all of the following:  Patients over the age of 18. Defect size: 2 to 10 cm^2 on the unilateral knee cartilage (up to 4 ㎤ in volume). Defect: isolated ICRS (International Cartilage Repair Society) grade III or IV single defect chondral lesion on articular cartilage with comparatively healthy cartilage present near the lesion site (ICRS Grade 1 ~ 2). Patients with a KOOS total score below 55. Patients able to walk without aid. Patients who agree to abide by rehabilitation protocols and follow-up programs, including residential exercise routines. Patients who provide written consent to the application of the clinical trial.  Exclusion Criteria:  Individuals who meet any of the following will be excluded from participation in this study:  Patients with inflammatory articular diseases such as rheumatoid arthritis or gouty arthritis. Patients scoring 3 or above on the Kellgren-Lawrence Grading Scale. Patients with inflammatory articular diseases related to autoimmune diseases. Patients hypersensitive to bovine derived proteins or any of the components in this product. Patients hypersensitive to Gentamycin. Patients with Haemophilia or markedly reduced immune function. Patients with arterial bleeding and severe venous bleeding. Patients with other diseases including tumors except for cartilaginous defects of joints. Patients with a history of radiation treatment and chemotherapy within the past two years. Patients who are pregnant, or nursing a baby. Patients who participate in concurrent clinical trials or previous clinical trials within 30 days of administration. Other cases where the investigator deems the patient ineligible for participation.