Inclusion Criteria:  couples presented with an extremely poor fertilization (<10%) couples with previous complete fertilization failure after undergoing ICSI cycles At least 4 MII oocytes Severe oligoasthinozoospermia Teratozoospermia testicular aspirated sperm  Exclusion Criteria:  age >40 years BMI >34 aspirated oocytes < 4 Normozoospermia subfertile patients