Comparison of I-Prf Impregnated Collagen With L-Prf in Terms of Postoperative Complications and Wound Healing After Lower Impacted Third Molar Teeth Surgery
Phase: NA
Conditions: Impacted Third Molar Tooth
Sponsor: Yuzuncu Yil University

02.09.2022 
 
Comparison of I-Prf Impregnated Collagen with L-Prf in terms of Postoperative 
Complications and Wound Healing After Lower Impacted Third Molar Teeth Surgery 
 
Levent Ciğerim DDS, PhD 1, Volkan KAPLAN, DDS, PhD2,Mehmet Güzel1 
1 Van Yuzuncu Yil University, Faculty of Dentistry, Department of Oral and Maxillofacial 
Surgery, Van, TURKEY 
2 Tekirdag Namık Kemal University, Faculty of Dentistry, Department of Oral and 
Maxillofacial Surgery, Tekirdag, TURKEY 
 
Corresponding author: Levent Ciğerim 
Address: Van Yüzüncü Yıl University, Faculty of Dentistry, Department of Oral and Maxillofacial 
Surgery, Van, Turkey Postal Code: 65080 
E-mail address: levent139@hotmail.com Phone:+905321633287 Office Phone: 904322251744  
Fax: +904322251747 
Levent CİĞERİM 
Van Yuzuncu Yil University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery 
levent139@hotmailmail.com, +905321633287, https://orcid.org/0000-0001-5218-8568 
Volkan KAPLAN 
Tekirdag Namık Kemal University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery  
dr.volkankaplan61@gmail.com, 05325041408, https://orcid.org/0000-0002-7605-1125 
Mehmet GÜZEL 
Van Yuzuncu Yil University, Faculty of Dentistry, Department of Oral and Maxillofacial Surgery 
dtmehmetguzel@gmail.com, +905363609854, https://orcid.org/0000-0002-9621-0496 
 
 

Study Protocol, Methods and Procedures 
The study is planned to be performed on a total of 50 patients, aged between 18-80 years, who 
applied to Van Yüzüncü Yıl University Faculty of Dentistry, Oral and Maxillofacial Surgery 
Department for extraction of lower impacted wisdom teeth with bilateral full mucosal or 
semi-bone retention. First, clinical and radiological evaluations will be made in patients. In 
the clinical evaluation, the systemic condition, tooth deficiency, occlusion and the condition 
of the impacted tooth will be evaluated. Panoramic film will be taken for radiographic 
evaluation, and periapical film will be taken if necessary. 
Individuals aged 18 and over, patients with semi-impacted or fully impacted wisdom teeth, 
patients without systemic disease (ASA0 and ASA1 individuals according to ASA 
classification), volunteers, individuals who did not use any medication in the last two weeks, 
and individuals without lower second molar tooth deficiency were included in the study. has 
been included. 
Individuals with painful temporomandibular joint disease, internal irregularity and/or 
inflammatory joint disease with a mouth opening of less than 25 mm, pregnant and lactating 
individuals, individuals who do not come to their postoperative controls, individuals using 
different drugs other than those recommended, individuals who are allergic to the study drugs 
and materials to be used are unable to work. will be excluded. 
It is planned as randomized, prospective, bilateral and single blind. 50 patients will be divided 
into two groups. 
First group (study group, one side of the same patients): Leukocyte platelet–rich fibrin (L-
PRF, 2700rpm,12 min); For the second group (control group, the other side of the same 
patient): type 1 collagen impregnated with injectable platelet-rich fibrin (I-PRF, 700rpm, 
3min) will be used. Both surgical fields will be sutured with 4\0 silk sutures (18mm, 3\8 
sharp, 75cm black). Which material will be used first by which side will be determined 
randomly. After 4 weeks, the other side will be operated. Platelet–rich fibrin (L-PRF) will be 
obtained by centrifugation at 2700 r.p.m. for 12 minutes and placed in the extraction socket 
within 2 minutes. Injectable platelet-rich fibrin (I-PRF) will be obtained by centrifugation at 
700 rpm for 3 minutes, drawn into the injector, absorbed into type 1 collagen and applied into 
the socket within a maximum of 2 minutes. 
Check-ups will be made in the first session of the patients, on the 2nd and 7th days after the 
operation, and in the 2nd and 4th weeks. After the operation in all patients; amoxicillin 
500mg(Largopen 500mg, 3*1), ibuprofen 600mg (Brufen 600 mg, 2*1), benzydamine HcL + 
chlorhexidine Gluconate mouthwash (Andorex 200ml mouthwash, 3*1) will be used 
routinely. Sutures will be removed after 1 week. Pain in patients will be evaluated with the 
VAS scale (at 3, 6, 12, and 24 hours and on days 2, 3, 4, 5, 6, and 7). For the evaluation of 
edema, measurements between Angulus-tragus, angulus-lateral canthus, angulus-nasalis, 
angulus-labial commissura and angulus-pogonion points will be made just before the 
operation, 2 days after the operation and 7 days after the operation. 

 
To assess trismus, mouth opening—the distance between the incisal edges of the central teeth 
at maximum mouth opening—will be measured immediately before, 2 days after, and 7 days 
after the operation. 
Soft tissue opening amount (in mm), plaque index, values and periodontal measurements of 
the second molar tooth in the incision line will be measured before the operation, 2 days after 
the operation and 7 days after the operation. Other postoperative complications will also be 
evaluated in the control sessions. Statistical analysis will be made and interpreted for the data 
obtained. 
Statistical Data Analysis: 
 Descriptive statistics for continuous variables in our study; As Average, Standard Deviation, 
Minimum and Maximum values; will be expressed as numbers and percentages for 
categorical variables. Mann-Whitney U analysis will be used to compare group means in 
terms of continuous independent variables. In addition, the Wicoxon test will be calculated in 
the comparison of double dependent groups. In order to determine the relationship between 
these variables, Spearman correlation coefficients will be calculated separately for the groups. 
Chi-square test will be used to determine the relationship between groups and categorical 
variables. Statistical significance level will be taken as 5% in calculations and SPSS statistical 
package program will be used for calculations.