Development of a Novel Functional Food Enriched With Beta-glucans Isolated From Edible Mushrooms of Greek Habitats
Phase: NA
Conditions: Healthy
Sponsor: Örebro University, Sweden

 
 
 
 
 
2022-06-29 
 
 
 
 
 
STUDY WITH TITLE: 
 
“Investigating effects in intestinal permeability of rich in 
beta-glucans Pleurotus eryngii mushrooms fermentation 
supernatants: an ex-vivo study” 
 
STUDY PROTOCOL 
 
By using a sigmoidoscopy procedure, colon biopsies will be collected from the subjects, where the collected 
biopsies will be mounted in Ussing Chambers in order to investigate the effects of the fibre fractions on 
intestinal permeability. Collected supernatants of Pleurotus eryngii mushrooms will be added to the mucosal 
side of the biopsy together with a stressor and two permeability markers. Samples will subsequently be 
collected from the serosal side and will be used to measure the permeability markers related to paracellular 
and transcellular permeability.  
 
 
STATISTICAL ANALYSIS 
 
All statistical tests will be assessed using One Way ANOVA with a nominal significance level of 5% and post hoc 
testing of treatment contrasts with Tukey’s HSD. The primary endpoint analysis will be based on fibre effect 
on the intestinal barrier function in terms of FITC passage, and whether there is a difference between 
treatments across all healthy subjects. Secondary endpoints include HRP quantification (supernatant), TEER 
values (electrophysiological parameter). 
 
Ö r e b r o  U n i v e r s i t y  
 
SCHOOL OF MEDICAL SCIENCE 
 
Nutrition -Gut -Brain Interactions Research Centre 
(NGBI)