Predictors of Cardiac Troponin Release After Tour de Borobudur 2017
Conditions: Heart Arrest, Sport Injury, Inflammatory Response, Oxidative Stress
Sponsor: Universitas Negeri Semarang

 
 
Tour de Borobudur Troponin Study on Predictors and Synergistic Role of 
MDA and Hs-CRP Levels (TdBTS) 
 
 
Tour de Borobudur Troponin Study 
ClinicalTrials.gov Identifier: NCT03310450 
 
 
Study Protocol 
 
12th October 2017 
 
 

Tour de Borobudur Troponin Study on Predictors and Synergistic Role of 
MDA and Hs-CRP Levels (TdBTS) 
 
Study Protocol 
 
General Procedure 
1. Study was conducted only with observation without any intervention. 
2. Data collection was done a day before cycling activity and immediate after cycling activity. 
3. Participants of 2017 Indonesia Tour de Borobudur (TdB) and North Coast (NC) who 
volunteer to participate in the study prior to cycling activity will be requested to complete 
data by filling out forms, conducted interviews, conducted doctor examination, 
measurement of weight, height, waist circumference, hips, Systolic Blood Pressure (SBP), 
Diastolic Blood Pressure (DBP), ECG examination and blood sampling. 
4. Immediately after cycling (at the finish), weight measurements, interviews, ECG 
examination, copying workout from Polar® heart rate monitor (HRM) and second blood 
sampling was done in the same manner. 
5. If the participant stops the cycling activity before the finish line, the post-race data was 
taken at the stop point as soon as possible. 
Interviews 
1. The interview was conducted by the research team officer to the TdB participants who 
became respondents. 
2. Interview was conducted pre- and post-cycling activities. 
3. Pre-cycling interviews was done to collect some data, i.e. identity and demographic data, 
training characteristics, medical history and family history with coronary artery diseases, 
personality and sleep quality. 
4. Immediate post-cycling interview was conducted to collect some data, i.e. the perceptions 
of the level of energy expenditure during cycling (rate of perceived exertion=RPE), 
subjective complaints and the type and amount of drink be taken. 
Anthropometry Measurement 
1. Anthropometry measurements were taken a day before the cycling activity, specifically the 
weight measurement done just before the start. 
2. Participants wore comfortable clothes. 
 
 

Physical Examination by Doctor 
1. The physical examination, SBP, DBP was done together with the measurement of 
anthropometry. 
2. Doctor's examination also states the participant was eligible or not to participate in cycling 
activities as a study participant. 
 
ECG 
1. ECG examination was done twice on the day before the cycling and immediately after 
completion of cycling activity (at the finish line). 
2. ECG examination was done by attaching leads at certain points on the chest and as well in 
wrists and ankles). 
3. To ensure the attachment is appropriate for both checks (pre- and post-race), the study 
participants will be marked with water-resist ink. 
Blood sampling 
1. Blood sampling was done twice; the day before cycling and immediately after completion 
of cycling activity (at the finish line). 
2. Blood sampling was performed for measuring Hb level, total cholesterol, HDL, hs-cTnI, 
hs-CRP and MDA levels. 
3. Blood sample then was examined in Biotechnology Laboratory (GAKI) FK UNDIP and 
Clinical Laboratory of Cito (group) Semarang 
Copying workout from Polar® heart rate monitor 
1. For participants that using Polar HRM, data retrieval was done by copying from the gadget. 
2. Doctor's examination also states the participant was eligible or not to participate in cycling 
activities as a study participant. 
Cycling activity 
1. Cycling activities follow the regulations set by the TdB or NC committee, which includes 
routes, obey the rules of traffic and other technical guidelines. 
2. Study participants conducted cycling activities at speeds according to their own ability and 
pace. 
3. Study participants were allowed to take a rest anytime and any length but should be 
recorded from the Polar HRM, by pressing the "PAUSE" button. 
4. Study participants were allowed to drink and eat as they wish but must record (remember) 
the amount and type of food/beverage consumed. 
5. Study participants who were unable to complete the race, when stopping their activity 
supposed to contact the research team whose phone number has been given, for the post-
race data collection and evacuation. 
 
 

Ethical clearance approval 
The study protocol was approved by the Ethical Committee of the Faculty of Medicine, 
University of Diponegoro/ Dr. Kariadi General Hospital, Semarang, Indonesia; and 
informed consent was obtained from all participants.