Effects of Blood Orange Juice Consumption on Endothelial Function in Healthy Overweight/Obese Men and Premenopausal Women of European Origin
Phase: NA
Conditions: Healthy, Overweight and Obesity
Sponsor: University of Leeds

Effects of Blood Orange Juice Consumption on Endothelial Function in Healthy 
Overweight/Obese Men and Premenopausal Women of European Origin 
 
 
 
 
 
Date: January 10, 2017 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

Sixteen overweight/obese participants of European origin will be asked to consume blood orange 
juice and a control drink for 2 weeks in a random sequence, separated by a 1-week washout 
period. All measurements will be conducted prior to and following 2-week consumption. 
Subjects will be instructed to stay fasted and restrain from exercise for 12 hours before 
measurements in the morning. Anthropometric measures will be conducted. After the subject is 
supine and comfortable for 15 minutes to reach a cardiovascular steady state, blood pressure will 
be measured in triplicate with 2-minute intervals. Endothelial function will be evaluated via 
brachial artery FMD. Each test for a given subject will be performed at the same time of the day. 
Following brachial FMD measurement, venous blood samples from antecubital vein will be 
collected into vacutainers to produce serum and plasma. Spot urine samples will also be 
collected.