A Proof-of-Concept Trial on the Effect of Ketamine on Fatigue
Phase: PHASE1
Conditions: Fatigue
Sponsor: National Institute of Nursing Research (NINR)

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Protocol Title: A Proof-of-Concept Trial on the Effect of Ketamine on Fatigue Abbreviated 
Title: Ketamine on Fatigue 
Protocol Number: 20NR0003  
Version Date: 04/18/2024 
 
NIH Principal Investigator: Leorey N. Saligan, PhD, RN, CRNP Symptom Management 
Branch 
National Institute of Nursing Research (NINR) Building 3, 
Room 5E14 
3 Center Dr. 
Bethesda, MD 20814 
Phone: 301-451-1685 
E-mail: saliganl@mail.nih.gov 
 
 

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TABLE OF CONTENTS  
TABLE OF CONTENTS ................................................................................................................ 2 
STATEMENT OF COMPLIANCE ................................................................................................ 5 
Precis ............................................................................................................................................... 6 
List of Abbreviations ...................................................................................................................... 8 
1 
Introduction and background .................................................................................................. 9 
1.1 
Fatigue in Chronic Illness ................................................................................................ 9 
1.2 
Mechanisms of Fatigue .................................................................................................. 10 
1.3 
Current Assessment and Treatment of Fatigue .............................................................. 11 
1.4 
Ketamine as Treatment for Fatigue ................................................................................ 11 
1.5 
Ketamine: Relevant Pharmacology ................................................................................ 12 
1.6 
Safety of Ketamine Administration ............................................................................... 12 
1.7 
Justification for the route and dose of ketamine used .................................................... 13 
2 
Study Objective ..................................................................................................................... 13 
2.1 
Primary objective ........................................................................................................... 13 
2.2 
Secondary objectives ...................................................................................................... 13 
3 
Subjects ................................................................................................................................. 14 
3.1 
Description of study populations ................................................................................... 14 
3.2 
Inclusion criteria ............................................................................................................. 14 
3.3 
Exclusion criteria............................................................................................................ 15 
4 
Study Design and Methods ................................................................................................... 18 
4.1 
Study overview ............................................................................................................... 18 
4.2 
Recruitment .................................................................................................................... 19 
4.3 
Screening ........................................................................................................................ 19 
4.4 
Screen Failures ............................................................................................................... 20 
4.5 
Study procedures ............................................................................................................ 20 
4.5.1 
Period 1 ................................................................................................................... 21 
4.5.2 
Period 2 ................................................................................................................... 22 
4.6 
Study Evaluations ........................................................................................................... 23 
4.6.1 
Fatigue Assessment:................................................................................................ 23 
4.6.2 
End of participation................................................................................................. 26 
5 
Management of Data and Samples ....................................................................................... 26 

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5.1 
Storage ............................................................................................................................ 26 
5.2 
Data and sample sharing plan......................................................................................... 26 
5.3 
Records Retention .......................................................................................................... 26 
5.4 
Certificate of Confidentiality ......................................................................................... 27 
6 
Additional Considerations .................................................................................................... 27 
6.1 
Research with investigational drugs or devices ............................................................. 27 
7 
Risks and Discomforts .......................................................................................................... 27 
8 
Subject Safety Monitoring .................................................................................................... 28 
8.1 
Study Drug Administration Monitoring: ........................................................................ 28 
9 
Outcome Measures................................................................................................................ 29 
9.1 
Primary outcome measures ............................................................................................ 30 
9.2 
Secondary outcome measures ........................................................................................ 30 
10 Statistical Analysis ................................................................................................................ 30 
10.1 
Analysis of data/ study outcomes ............................................................................... 30 
10.2 
Analysis of Secondary Endpoints ............................................................................... 30 
10.3 
Power analysis ............................................................................................................ 31 
11 Human Subjects Protection ................................................................................................... 32 
11.1 
Subject selection ......................................................................................................... 32 
11.2 
Justification for exclusion of children ........................................................................ 32 
11.3 
Justification for exclusion of other vulnerable subjects ............................................. 32 
11.3.1 Pregnant and lactating individuals: ......................................................................... 32 
11.3.2 Impaired consent capacity: ..................................................................................... 32 
11.4 
Justification of sensitive procedures ........................................................................... 33 
11.4.1 Active comparator arm: .......................................................................................... 33 
11.4.2 Drug and alcohol-free period: ................................................................................. 33 
11.5 
Safeguards for vulnerable populations ....................................................................... 33 
11.5.1 Requirement of a primary care provider for study participation: ........................... 33 
11.5.2 NIH staff: ................................................................................................................ 33 
12 Anticipated Benefit ............................................................................................................... 34 
13 Consent Documents and Process .......................................................................................... 34 
13.1 
Designation of those obtaining consent ...................................................................... 34 
13.2 
Consent procedures..................................................................................................... 34 

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13.3 
Consent documents ..................................................................................................... 35 
14 Data and Safety Monitoring .................................................................................................. 35 
14.1 
Data and safety monitor .............................................................................................. 35 
14.2 
Data and safety monitoring plan ................................................................................. 35 
14.3 
Criteria for stopping the study or suspending enrollment or procedures ................... 36 
15 Quality Assurance (QA) ....................................................................................................... 36 
15.1 
Quality assurance monitor .......................................................................................... 36 
15.2 
Quality assurance plan ................................................................................................ 36 
16 Reporting of Unanticipated Problems, Adverse Events and Protocol Deviations ................ 36 
17 Alternatives to Participation ................................................................................................. 36 
18 Privacy .................................................................................................................................. 36 
19 Confidentiality ...................................................................................................................... 36 
19.1 
For research data and investigator medical records .................................................... 36 
19.2 
For stored samples ...................................................................................................... 37 
19.3 
Special precautions ..................................................................................................... 37 
20 Conflict of Interest ................................................................................................................ 37 
21 Technology Transfer ............................................................................................................. 37 
22 Research and Travel Compensation...................................................................................... 37 
23 References ............................................................................................................................. 40 
24 Attachments/Appendices ...................................................................................................... 46 
 
 
 

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STATEMENT OF COMPLIANCE 
The trial will be carried out in accordance with International Council on Harmonisation Good 
Clinical Practice (ICH GCP) and the following:  
• United States (US) Code of Federal Regulations (CFR) applicable to clinical studies (45 
CFR Part 46, 21 CFR Part 50, 21 CFR Part 56, 21 CFR Part 312, and/or 21 CFR Part 812)  
National Institutes of Health (NIH)-funded investigators and clinical trial site staff who are 
responsible for the conduct, management, or oversight of NIH-funded clinical trials have 
completed Human Subjects Protection and ICH GCP Training. 
The protocol, informed consent form(s), recruitment materials, and all participant materials will 
be submitted to the Institutional Review Board (IRB) for review and approval.  Approval of both 
the protocol and the consent form must be obtained before any participant is enrolled.  Any 
amendment to the protocol will require review and approval by the IRB before the changes are 
implemented to the study.  In addition, all changes to the consent form will be IRB-approved; an 
IRB determination will be made regarding whether a new consent needs to be obtained from 
participants who provided consent, using a previously approved consent form. 
 
 

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PRECIS 
Purpose: The purpose of the study is to investigate the anti-fatigue effects of ketamine in 
individuals with chronic illness. 
Background: Although the underlying mechanisms of fatigue have been studied in several 
disease conditions (Bower et al., 2002; Brola et al., 2007), the etiology, mechanisms, and risk 
factors remain elusive and this symptom remains poorly managed. Fatigue is conceptualized as a 
multidimensional symptom which incorporates temporal, sensory, cognitive/mental, 
affective/emotional, behavioral, and physiological dimensions (Voss, et al., 2006). It is described 
as a common, chronic, and disabling symptom in individuals with Sjögren’s syndrome (Ng et al., 
2012) and those with systemic lupus erythematosus (Fonsecaet al., 2014). We recently observed 
that upregulation of glutamate receptors (e.g., GRM5) can predict individuals who will develop 
chronic fatigue one year after completing cancer therapy, suggesting that fatigue may share 
common glutamatergic markers with depression .Ketamine is an N-methyl-D-aspartate (NMDA) 
receptor antagonist and has been reported to have rapid anti-depressant effects (Berman et al., 
2000; Prommer, 2012; Aan Het Rot etal., 2012), and we recently found that it also has rapid anti- 
fatigue effects (Saligan et al., 2016). Evidence suggest that severe fatigue in diverse medical 
conditions is driven by similar biological mechanisms, hence identifying a potential anti-fatigue 
agent in one medical condition may be a valuable anti-fatigue therapy for other fatiguing 
conditions. 
Population for Study: This proof-of-concept study will enroll 59 individuals (target n of 
completers 
= 50) with chronic fatigue. 
Key Inclusion/Exclusion Criteria: Participants must have a fatigue visual analog scale (VAS) 
score of ≥ 50 mm (on a 0-100 mm horizontal scale). The ≥ 50 mm fatigue VAS score is 
considered clinically important fatigue cutoff score for patients with chronic illness (Klokkerud 
et al., 2017; Minnock et al., 2009), and also captures the effectivity outcome of a previous 
pharmacologic intervention for fatigue (Kamath et al., 2012). The participants must not have any 
progressive or unstable conditions or be taking medications that cause fatigue. 
Methodology: This is a phase II, randomized, double-blind (study team and participants), active 
comparator- controlled, cross-over trial. After determining eligibility during the screening visit, 
the participant will be randomized to determine the sequence of study drug/active comparator to 
take during each phase. 
Main Study Events / Estimates of Duration and Time Commitments: The study has two periods, 
and each period is approximately two weeks long (total of four weeks). The study (both periods, 
excluding the screening visit) will require eight NIH outpatient visits and three phone calls. 
Primary and Representative Secondary Outcomes: The primary outcome measure of the study is 
the change in self-reported fatigue VAS score before and three days after receiving ketamine or 
active comparator (midazolam). A 20% decrease in fatigue VAS score three days after ketamine 
treatment will be considered the primary indicator of efficacy in this study. The secondary 
outcomes of this study include: symptoms, physical activity count, skeletal muscle strength, 
motivation score, cognitive function test scores, changes in gene expression or protein levels of 
pro- inflammatory markers (e.g., lymphotoxin, IFNγ, TNFα) typically seen in fatigue, and 

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neurometabolite (e.g., BDNF) levels and mitochondrial markers (e.g., glucose transporter 4) 
before and after a dose of ketamine or active comparator. 
General Analytic Plans: A linear mixed model with restricted maximum likelihood estimation 
will be used to examine changes in fatigue symptoms over the course of the trial where all 
participants with at least a pre-dose and one post-dose measure will be included. Within-subjects 
factors will include time with pre-dose and all other points. The interaction between time and 
ketamine treatment will be included along with the fixed intercept. Multiple test corrections (e.g., 
Bonferroni post hoc tests) will be used to examine differences between levels of significant 
effects. 
The primary outcome measure of the study is the change in fatigue score as measured by the 
self- reported fatigue instrument. 
 
 

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LIST OF ABBREVIATIONS 
ACAT Ability to Consent Assessment Team 
ACLS Advanced Cardiac Life Support 
AE 
Adverse Event 
AIDS Acquired Immunodeficiency Syndrome 
AMPA α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid 
BDNF Brain Derived Neurotrophic Factor 
CAT Computer Adaptive Test 
CBC Complete Blood Count 
CD 
Clinical Director 
CFS 
Chronic Fatigue Syndrome 
CNS Central Nervous System 
COI 
Conflict of Interest 
CRF 
Cancer Related Fatigue 
CRIS Clinical Research Information System 
CRP 
C-Reactive Protein 
CSRP Committee for the Scientific Review of Protocols 
DBP Diastolic Blood Pressure 
DSM-5 
Diagnostic and Statistical Manual 
DSMB Data and Safety Monitoring Board 
DSMP Data and Safety Monitoring Plan 
EEfRT Effort-Expenditure for Rewards Task 
FACIT-F 
Functional Assessment of Chronic Illness Therapy – Fatigue 
FDA Food and Drug Administration 
HAM-D 
Hamilton Depression 
HIV 
Human Immunodeficiency Virus 
HRPP Human Research Protection Program 
HSP90α 
Heat Shock Proteins 
HSPU Human Subjects Protection Unit 
IRB 
Institutional Review Board 
IND 
Investigational New Drug 
IOM 
Institute of Medicine 
LIP 
Licensed Independent Practitioner 
ME/CFS 
Myalgic encephalomyelitis/Chronic Fatigue Syndrome 
MGH- CPFQ Massachusetts General Hospital Cognitive and Physical Functioning 
Questionnaire 
NCCN National Comprehensive Cancer Network 
NIH 
National Institutes of Health 
NIMH National Institute of Mental Health 
NINR National Institute of Nursing Research 
NMDA 
N-methyl-D-aspartate 
 
 

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1 
INTRODUCTION AND BACKGROUND 
In primary care settings, 42% of patients are impacted by fatigue; women are affected more 
frequently than men (Pawlikowska et al., 1994; Ricci et al., 2007; Sharpe & Wilks, 2002). 
Primary care physicians treat a minimum of one patient with fatigue symptoms for every day of 
the week (Katon et al., 1991). Yet, fatigue symptoms in 25% of patients go undiagnosed in 
primary care settings (L. B. Krupp, 2003; Wessely et al., 1999). 
Furthermore, fatigue symptoms also negatively affect functioning including quality of life, 
activities of daily living, employment, and psychological well-being (Hann et al., 1999; 
Pawlikowska et al., 1994; Robinson et al., 2014). 
1.1 
FATIGUE IN CHRONIC ILLNESS 
Fatigue is one of the most common complaints in chronic illnesses, and many patientsdescribe it 
as one of the most debilitating symptoms experienced (Arnold, 2008; Eckhardt, et al., 2014; 
Minnock et al., 2009; Ramsey-Goldman & Rothrock, 2010). In chronic conditions, fatigue 
impacts up to 67 to 90% of individuals (Fonseca et al., 2014; Krupp etal., 1990; Pollard et al., 
2006). 
The table below describes the prevalence rates of fatigue reported by individuals with different 
medical conditions. 
Table 1: Fatigue Prevalence Rates in Various Clinical Populations 
 
Disease 
Percent Fatigue Reported 
AIDS 
85% 
Cancer undergoing radiation therapy 
75-100% 
Cancer undergoing chemotherapy 
60-90% 
Cancer untreated 
78% 
Parkinson's Disease 
60% 
Epilepsy 
44% 
Multiple Sclerosis 
97% 
Systemic Lupus Erythematosus 
85.70% 
Rheumatoid Arthritis 
70% 
Primary Biliary Cirrhosis 
64-81% 
Post-Lyme Disease 
82% 
Postoperative Hysterectomy 
75% 
Remitted Major Depressive Disorder 
40% 
 
The fatigue experience of individuals with chronic conditions can be distinguished from ‘normal’ 
everyday tiredness as being more frequent, unrelenting, unpredictable and unresolved by rest 
(Dittner et al., 2004; Hewlett et al., 2005). Patients are adamant that fatigue is different from 
tiredness, because tiredness resolves with rest, but fatigue does not. Fatigue is also described as 

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extreme fluctuations in lack of mental, physical, and cognitive energy from day to day and 
throughout each day. Fatigue is often a chronic, unwavering, persistent, disabling problem in 
patients with chronic conditions. Severe fatigue is reported in up to 57% of patients with single 
inflammatory rheumatic disease (rheumatoid arthritis, systemic lupus erythematosus, ankylosing 
spondylitis, Sjögren's syndrome, psoriatic arthritis, and scleroderma) and 82% in patients with 
fibromyalgia (Overman et al., 2016). A recent article revealed that about 35% of cancer 
survivors complained of disabling fatigue one year after completing cancer treatment and about 
25%continue to suffer from persistent fatigue two years or more after completing their treatment 
(van Leeuwen et al., 2018). Fatigue is one of the most common and debilitating symptom 
experienced by patients with cancer (Wang, 2008). For myalgia encephalomyelitis/chronic 
fatigue syndrome (ME/CFS) patients, at least one-quarter of them are house- or bed-bound at 
some point in their lives (White et al., 1998). Fatigue symptoms for both conditions (cancer 
survivors and ME/CFS) have significant repercussions for both direct and indirect health 
economic outcomes (Carlotto et al., 2013;Jason et al., 2008). 
ME/CFS often is seen as a diagnosis of exclusion, which also can lead to delays in diagnosis or 
misdiagnosis of a psychological problem (Fossey et al., 2004; Bayliss et al., 2014). The 
commonly used diagnostic criteria for ME/CFS is the 2015 Institute of Medicine (IOM) 
Diagnostic Criteria (Clayton, E.M., 2015). Cancer-related fatigue (CRF)is determined by self- 
report and there is currently no approved International Classification of Diseases diagnostic 
criteria/code for CRF. 
1.2 
MECHANISMS OF FATIGUE  
There are several hypotheses that may offer insight into the etiology of fatigue across medical 
conditions, including in CRF and ME/CFS. The causes and mechanisms of fatigue are complex 
and multifactorial, and are influenced by somatic, affective, cognitive and psychosocial factors. 
Below are possible pathophysiological factors underlying fatigue in chronic illness. 
1. Autonomic dysfunction: Fatigue is believed to be associated with decreased 
parasympathetic activity (Koh et al., 2017). 
2. Metabolic pathways: A global gene expression analysis of whole blood from a group of 
patients with chronic illness revealed that high fatigue was associated with differential 
expression of genes involved in metabolic pathways specifically with actin filaments and 
migration of cells (James et al., 2015; Pihur et al.,2011). 
3. Neurophysiological abnormalities: 
a. Oxidative stress: Fatigue is thought to result from an accumulation of oxidative 
and nitrosative stress (O&NS), which can induce muscle fatigue, as well as 
O&NS-induced defects in the sodium-potassium pump, a critical system for 
neuromotor synaptic transmission (Morris et al., 2016). 
b. Mitochondrial Function: It is also thought that fatigue is associated with 
mitochondrial dysfunction through the downregulation of the mitochondria 
lbiogenesis master gene, peroxisome proliferator-activated receptor gamma 
coactivator 1-alpha, which shifts cellular metabolism to glycolysis causing abuild 
up of toxic metabolites within monocytes (Morris et al., 2016). 
c. Immune modulatory function: Fatigue is generally associated with a disarray in 
the concentrations of inflammatory cytokines (Morris et al., 2016; Tripp et al., 
2016), which is believed to be a consequence of the activation of the Toll-Like 

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Receptor Cycle through pathogen-associated and damage-associated molecular 
patterns, which include heat shock proteins (Morris et al., 2016). One study even 
showed higher plasma levels of heat shock proteins (HSP90α) with high fatigue 
compared to those with low fatigue (Bårdsen et al., 2016). A genome-
wideepigenetic investigation revealed that pronounced methylation of CpG sites 
of genes involved in the regulation of immune response and inflammation (e.g., 
lymphotoxin α) are observed in individuals with high fatigue (Brække Norheim 
etal., 2016). 
Our recent evidence showed that activation of glutamate receptors in T cells mediated the 
development of chronic fatigue in patients who completed radiation therapy for non- metastatic 
prostate cancer (Feng et al., 2018). In fact, the activation of glutamate receptor samplified NF-κB 
activation and inhibition of glutamate receptors reduced pro-inflammatory cytokine production 
(Feng et al., 2018), suggesting a role of mGluR5 in modulating T cell activation. This proof-of- 
concept study will use glutamate receptors as a therapeutic target for fatigue. It will provide 
initial evidence that ketamine relieves fatigue symptoms in individuals across medical 
conditions. Considering that the action ofketamine on glutamatergic receptors (e.g., NMDA and 
AMPA) has been identified (Iadarola et al., 2015), findings from this study will be valuable to 
start confirming the hypothesis mentioned above to further understand the shared mechanism 
underlying the fatigue experience across medical conditions. 
1.3 
CURRENT ASSESSMENT AND TREATMENT OF FATIGUE 
There are several instruments used to best measure fatigue. Currently, unidimensional and 
multidimensional scales are sufficient and appropriate measurements to specifically identify 
fatigued patients. The unidimensional scales measure the physical impact of fatigue and are the 
most widely used scales (Minton and Stone, 2009). Multidimensional scales are more extensive 
and can cover anywhere between two and five different aspectsof fatigue but are difficult to use 
due to time limitations and burden on the patient (Portenoy and Itri, 1999; Minton and Stone, 
2009). Objective measures of physical activity and performance (e.g., actigraphy), as well as 
cognitive function are also used to assess fatigue (Dassouki et al., 2016). 
Treatment and management for fatigue symptoms has been attempted by pharmacological and 
non-pharmacological interventions. No United States (US) Food and Drug Administration 
(FDA) medication has been approved to treat fatigue, in general. A previous study showed 
inconsistent results of Rituximab treatment in reducing physical fatigue in Sjögren's Syndrome 
patients (Arends et al., 2016; Bowman et al., 2017). Erythropoietin, megestrol acetate, 
psychostimulants, and methylphenidate have been used as anti-fatigue treatments in cancer 
patients, and showed inconsistent results (Portenoy and Itri, 1999; Mock, 2004). Recent meta-
analysis revealed that exercise and cognitive therapy are more effective in reducing cancer-
related fatigue than pharmacological agents (Mustian et al., 2017). More investigations to 
understand the biology of fatigue are warranted to identify optimal therapies for this distressing 
problem. 
1.4 
KETAMINE AS TREATMENT FOR FATIGUE 

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We recently observed that ketamine (0.5 mg/kg single intravenous dose) has rapid anti-fatigue 
effects reported by patients 40 minutes after infusion and lasting until two days post infusion 
(Saligan et al., 2016). In fact, the effect size of the ketamine-placebo difference was greatest at 
day 2 (d = 0.59). Figure 1 below describes this anti-fatigue effect of ketamine 
Figure 1 Anti-Fatigue Effects of Ketamine. 
This proof of concept study aims to provide initial evidence that a sub anesthetic dose of 
ketamine has rapid anti-fatigue effects. It will also explore the biologic mechanisms of 
ketamine’s anti- fatigue effects hoping to advance our understanding of the biologic 
underpinnings of chronic fatigue. Therefore, the information obtained from this study will have 
several clinical implications both in research and community settings: (1) ketamine, at a low 
dose, may be effective in rapidly treating fatigue, which can change clinical management for this 
debilitating symptom; (2) exploring the biologic mechanisms of the anti-fatigue effects of 
ketamine in this study may reveal pharmacodynamically-active therapeutic targets; and (3) 
confirmation of the rapid anti- fatigue effects of ketamine may provide opportunities for future 
exploratory investigations of other long acting anti-fatigue therapies or the anti-fatigue effects of 
repeated doses of ketamine. 
1.5 
KETAMINE: RELEVANT PHARMACOLOGY 
Initially, ketamine is distributed to highly perfused tissues, including brain, to achieve levels four 
to five times that in plasma. Ketamine has high lipid solubility and low plasma protein binding 
(12%), which facilitates rapid transfer across the blood-brain- barrier. The distribution half-life is 
approximately 10 minutes. Biotransformation of ketamine into multiple metabolites involves N- 
demethylation by cytochromes p450 to nor ketamine, an active metabolite with a potency 
approximately one-third that of ketamine. Nor ketamine is then hydroxylated and conjugated to 
water-soluble compounds that are excreted in urine. Elimination occurs primarily via the kidney, 
with only a small percentage recovered in the urine as unchanged drug (Chang et al., 1970). The 
elimination half-life I sapproximately two hours which is secondary to the combination of rapid 
clearance and large volume of distribution (Domino et al., 1984). 
1.6 
SAFETY OF KETAMINE ADMINISTRATION 

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Ketamine is a general anesthetic for human and veterinary use. Ketamine has been administered 
as an anesthetic to several million adults and children over the last few decades and has a good 
safety profile. Ketamine exerts sympathomimetic activity and may produce mild to moderate 
increases in blood pressure, heart rate, and cardiac output. The reported incidence of perceptual 
disturbances varies from less than 5% to greater than 30%(Knox et al., 1970; White et al., 1980). 
When such reactions occur, they are usually mild and short-lived (Green and Johnson 1990). No 
strong evidence exists from long-term studies suggesting that participants exposed to ketamine 
were at greater risk of abusing iton follow-up. The associate investigator of this study and his 
team, Dr. Carlos 
A. Zarate, Jr. has extensive experience with ketamine, having infused (sub-anesthetic doses) it in 
patients with treatment-resistant depression under protocol 04-M-0222. This study will continue 
to use the same dose of ketamine used in the 04-M-0222 protocol, which was found to be safe. 
Similar exclusion criteria used by 04-M-0222 will be adapted in this protocol to avoid 
interactions of ketamine with other medications. The lists of medications that participants can 
take and still be able to safely receive ketamine are listed in Section 3. 
1.7 
JUSTIFICATION FOR THE ROUTE AND DOSE OF KETAMINE USED 
The sub-anesthetic dose of ketamine was selected on the basis of previous published reports 
describing its safety (Domino et al., 1965; Corssen & Domino 1966; Krystal et al., 1994; Lahti et 
al., 1995; Krystal et al., 1998; Carpenter, 1999; Krystal et al., 1999; Krystal et al., 2000; Krystal 
and D'Souza, 2001; Krystal et al., 2002; Krystal et al., 2003a; Krystal et al., 2003b), and its 
antidepressant effects (Berman et al., 2000; Zarate et al., 2006). We will use the same dose of 
ketamine, 0.5 mg/kg infused over 40 minutes. 
2 
STUDY OBJECTIVE 
2.1 
PRIMARY OBJECTIVE 
This study will determine the effect of a single intravenous dose of ketamine (0.5 mg/kg) in 
reducing clinically significant fatigue in individuals with chronic illness. 
Hypothesis: A single intravenous dose of ketamine will reduce clinically significant fatigue in 
individuals with chronic illness. 
2.2 
SECONDARY OBJECTIVES 
a. To determine the effect of a single intravenous dose of ketamine on symptoms, 
physical activity, motivation, skeletal muscle strength, and cognitive functioning 
of study participants.  
i. Hypothesis: A single intravenous dose of ketamine will reduce fatigue-
associated symptoms and improve physical activity, motivation, skeletal 
muscle strength, and cognitive functioning of individuals with chronic 
fatigue. 
b. To determine whether a single intravenous dose of ketamine alters gene 
expression or the protein levels of pro-inflammatory markers (e.g., lymphotoxin, 
IFNγ, TNFα) typically seen in fatigue, neurometabolites (e.g., brain derived 
neurotrophic factor [BDNF], glutamatergic/dopaminergic markers), and 
mitochondrial markers (e.g., metabotropic glutamate receptors) in the blood. 

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i. Hypothesis: The change in clinically significant fatigue experienced by 
individuals with chronic illness after a single intravenous dose of ketamine 
will be associated with changes in gene expression, levels of pro-
inflammatory cytokines, neurometabolites, and mitochondrial markers in 
the blood. These levels in blood before and after treatment with ketamine 
or active comparator will also be related to the symptom scores. 
As a proof-of-concept study, the selection criteria listed below should not be affected by the 
heterogeneity of factors that cause fatigue, as described in the introduction. The primary outcome 
will be assessed by changes in fatigue as measured by the visual analog scale, which will provide 
initial evidence if the anti-fatigue effects of ketamine is observed across medical conditions. The 
secondary outcomes of the study will inform the biological underpinnings of this anti-fatigue 
effect of ketamine, which can advance our understanding of fatigue’s etiology. 
3 
SUBJECTS 
3.1 
DESCRIPTION OF STUDY POPULATIONS 
This protocol will enroll 59 individuals (target n of completers = 50), who are cancer survivors 
or with chronic illness (e.g., ME/CFS, Sjogren’s disease, systemic lupus erythematosus [SLE]) 
with significant fatigue (Fatigue VAS score of ≥ 50 mm on 0-100 mm horizontal scale). This 
cut-off fatigue VAS score captured clinically meaningful fatigue experience in other clinical 
populations, such as in healthy adults (fatigue numeric rating scale [FNS] mean = 5.43 ± 2.26, 
Kim & Abraham, 2017), those with musculoskeletal diseases (FNS mean = 6.1, confidence 
interval [5.8, 6.4], Klokkerud et al., 2017), and rheumatoid arthritis (FNS mean = 6.7 +/- 2.1, 
Minnock, et al., 2009). Drop-outs and participants who withdraw will be replaced to attain the 
target n = 50 study completers. 
3.2 
INCLUSION CRITERIA 
1. Have chronic, persistent fatigue for at least 6 months; 
a) Intensity = ≥ 50 mm using fatigue VAS (on a 0-100 mm horizontal fatigue scale). 
b) Chronicity = > six months total in the past year using the first item of the revised Piper 
Fatigue Scale. 
2. Be a cancer survivor with a documented medical report of completing primary 
cancer treatment > 6 months ago (except hormone and vaccine therapies) OR diagnosed with 
complex syndromes like ME/CFS, CFS, chronic fatigue, fibromyalgia; OR autoimmune disorder 
such as SLE, or Sjogren’s disease; 
3. Able to provide written informed consent; 
4. Able to have an accompanying responsible adult for drug infusion study visits; 
5. 18 – 70 years of age at the time of signing the informed consent form; 
6. Participants may be NIH employees/staff (see below for some exclusion); 
7. Individuals of childbearing potential must use adequate contraception, as defined below, prior 
to study entry and for the duration of study participation. Sexually active subjects must agree to 

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use at least one medically accepted barrier method of contraception during the study. For 
example: 
• 
Condoms 
• 
Prescribed hormonal oral contraceptives, vaginal ring, or transdermal patch. 
• 
Intrauterine device (IUD). 
• 
Intrauterine hormone-releasing system (IUS). 
• 
Depot/implantable hormone (e.g., Depo-provera®, Implanon). 
• 
Bilateral tubal occlusion/ligation. 
• 
Sexual abstinence: refraining from intercourse during the entire period of risk 
associated with the study requirements or if the participant decides to become 
sexually active during the study, then one of the highly effective birth control 
methods must be used. 
8. Individuals of non-childbearing potential as defined by the following criteria: 
• 
Postmenopausal defined as 12 months of spontaneous amenorrhea or follicle- 
stimulating hormone (FSH) serum level > 40mIU/mL; appropriate 
documentation is required. 
• 
Surgically sterile by hysterectomy and/or bilateral oophorectomy with 
appropriate documentation of surgical procedure. 
• 
Has a congenital condition resulting in no uterus. 
OR 
• 
Is sterile 
• 
Has documentation confirming vasectomy 
3.3 
EXCLUSION CRITERIA 
1. Total body irradiation or cranial irradiation for cancer; 
2. Has a diagnosis of progressive or unstable disease to any body system causing clinically 
significant fatigue (e.g., class IV congestive heart failure, end-stage renal disease, liver failure, 
stage IV chronic obstructive pulmonary disease) including patients with active systemic 
infections (e.g., human immunodeficiency virus (HIV), active hepatitis, COVID-19 – screened 
using NIH Clinical Center questionnaire); 
3. Individuals with comorbid conditions other than clinically stable cardiovascular, metabolic 
conditions, and rheumatologic/systemic autoimmune diseases; 
4. Current or past psychiatric disorders including medically documented depression with 
psychosis, bipolar disorder, schizophrenia; 
5. Clinically documented post-traumatic stress syndrome and/or traumatic brain injury because 
of the high risk for ketamine to exacerbate symptoms including hallucinations; 
6. Categorized as a high-risk drinker (≥5 drinks/day and ≥15 drinks/week for men, ≥4 drinks/day 
and ≥8 drinks/week for women). (“Dietary Guidelines for Americans 2015- 2020,” U.S. 
Department of Health and Human Services and U.S. Department of Agriculture); 
7. Detectable alcohol content >1 mg/dL using either breath test or using other biologic samples 
(e.g., urine); 

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8. Current substance use disorder within the last five years as diagnosed on the Structured 
Clinical Interview for DSM-5 (SCID-5) or positive urine toxicology results at enrollment; 
9. Participants with clinical hypothyroidism or hyperthyroidism defined by abnormal thyroid 
stimulating hormone TSH; 
10. Poorly controlled hypertension as judged by the Principal Investigator and confirmed by 
repeat assessment during the screening period (SBP >160 and DBP > 100 in all readings); 
11. Any medical condition causing impairment in mobility (e.g., stroke with residual 
neuromuscular weakness). This may prohibit the assessment of study outcomes, such as physical 
activity; 
12. Any change in dose of regularly scheduled medication or initiation of a new medication 
(excluding PRN medications) within four weeks prior to signing the informed consent form and 
throughout the entire duration of the study; 
13. Untreated sleep condition.  
14. Medically diagnosed kidney disease (except for chronic stable kidney disease with 
eGFR>45); 
15. Medically diagnosed acute narrow-angle glaucoma; 
16. Allergic to ketamine, benzodiazepines, flumazenil; 
17. With poor IV access; 
18. NINR employees or subordinates, relatives, and/or co-workers of NINR employees/staff or 
study investigators; 
19. Pregnant or lactating individuals; 
20. Ongoing medical condition that is deemed by the Principal Investigator to interfere with the 
conduct or assessments of the study or safety of the participant. 
21. Taking concomitant medication known to interact with ketamine and/or midazolam 14 days 
prior to study drug administration and during the study. The medications are shown in the tables 
below: 
List of Psychiatric Medications Allowed and Not Allowed During the Study* 
Drug Class 
Episodic Use 
(as needed) 
Chronic 
Use 
Restrictions 
Antidepressants 
No 
No 
SSRI, SNRI, and serotonin 
modulators, including 
Buproprion are allowed if on 
low maintenance doses, and no 
history of seizure if taking 
SNRI. 
Antipsychotics 
No 
No 
Anxiolytics 
No 
No 
Mood Stabilizers 
No 
No 

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Psychotropic drugs not 
otherwise specified 
(including herbal products) 
No 
No 
No drugs with psychomotor effects or 
with anxiolytic, stimulant, antipsychotic, 
or sedative properties are allowed. 
Sedatives/Hypnotics 
No 
No 
List of Non-Psychiatric Medications Allowed and Not Allowed During the Study* 
Drug Class 
Episodic Use 
(as needed) 
Chronic 
Use 
Restrictions 
Analgesics 
Y 
N 
Non-narcotic analgesics only 
Anorexics (sibuteramine) 
N 
N 
Antacids 
Y 
Y 
Antianginal Agents 
N 
N 
Antiarrhythmics 
N 
N 
Antiasthma Agents 
Y 
Y 
Systemic corticosteroids are not allowed if 
Antibiotics 
Y 
N 
Except erythromycin (see P450-3A4 
Anticholinergics 
N 
N 
Anticoagulants 
N 
N 
Anticonvulsants 
N 
N 
Carbamazepine, phenytoin, and 
oxcarbazepine are 3A4 inducers and 
significantly decrease perampanel levels. 
Topiramate may increase perampanel 
Antidiarrheal Preparations 
Y 
N 
 
Drug Class 
Episodic Use 
(as needed) 
Chronic 
Use 
Restrictions 
Antifungal Agents 
Systemic 
N 
N 
Topical 
Y 
Y 
Antihistamines 
Nonsedating 
Sedating 
Y 
N 
Y 
N 
Antihypertensives 
Y 
Y 
Anti-inflammatory Drugs 
Y 
Ya 
Systemic corticosteroids are not allowed 
if taking more than > 10 mg /day of 
prednisone or glucocorticoid equivalent. 
Antinauseants 
Y 
Y 
Antineoplastics 
N 
N 
Agents used in low maintenance doses as 
immunosuppressive agents (not as anti- 
neoplastics) such as Azathioprine, 
Methotrexate, Mycophenolate mofetil, 
and 
Belimumab are allowed. 
Antiobesity 
N 
N 
Antivirals 
N 
N 
Except for treatment of HSV with agents 
without CNSactivity e.g. acyclovir, 
ganciclovir, famciclovir, valacyclovir 

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Cough/Cold Preparations 
Y 
N 
Dextromethorpha 
n preps- N/N 
Guaifenesin - 
Y/Y 
Pseudoephedrine 
- N/N 
Diuretics 
Y 
Yb 
H2-Blockers/ PPI 
Y 
Yb 
Except cimetidine (see P450-3A4 
enzyme inhibitorsbelow) 
Hormones 
N 
Yb 
Only thyroid hormone replacement, 
oral contraceptives, and estrogen 
replacement therapy areallowed. 
Hypoglycemic Agents 
N 
Yb 
Only oral hypoglycemic agents are 
allowed. 
Antihyperlipidemics 
N 
Yb 
Insulin 
N 
N 
Laxatives 
Y 
Y 
Muscle Relaxants 
N 
N 
P450-3A4 enzyme inhibitors 
N 
N 
Including cimetidine, 
erythromycin, diltiazem, 
verapamil, ketoconazole, and 
itraconazole (topical 
ketoconazole allowed) 
Protease Inhibitor 
N 
N 
Including Saquinavir 
a Allowed only if being taken prior to enrolling in the study. 
b Allowed only if being taken for at least 2 months prior to enrolling in the study and the dose has been stable for at 
least 1 month. 
*Some medications in the above table may be indicated for exclusionary conditions; therefore, it would be unlikely 
that participants meeting inclusion will be taking them. 
4 
STUDY DESIGN AND METHODS 
4.1 
STUDY OVERVIEW 
While fatigue is generally a variable phenomenon, we will be enrolling individuals with chronic 
illness (e.g., cancer survivors and ME/CFS) reporting chronic, persistent, significant fatigue for 
at least six months. We assume that significant fatigue will persist over the duration of the study. 
To reduce variability, we will be limiting co-morbid conditions to clinically stable cardiovascular 
and metabolic conditions. 
Therefore, this study will utilize a double-blind (study team and participants will be blinded), 
active comparator-controlled, cross-over design. This design was used by the original National 
Institute of Mental Health (NIMH) study (Zarate et al., 2006), where the initial anti-fatigue 
effects of ketamine were discovered (Saligan et al., 2016). The NIMH study was used to put 
together the first anti-fatigue ketamine study (15-NR-0037), which was approved by the NIH 
Combined Neuroscience IRB in 2015. The original study (Zarate et al., 2006) showed that 
ketamine’s antidepressant and anti-fatigue started 40 minutes after ketamine infusion and lasted 
two days after ketamine infusion (Saligan et al., 2016). 

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The study will require eight National Institutes of Health (NIH) outpatient clinic visits. In 
addition, there is a screening visit to determine eligibility and three follow-up phone calls. All 
tests and study visits will be conducted at the NIH Clinical Research Center. 
For participants enrolled in other NIH treatment protocols, when possible, all tests and blood 
draws will be coordinated around the participant’s clinical care procedures so that unnecessary 
duplication of tests can be avoided and inconvenience to participants is minimized. The 
procedures involved in all the study time points are discussed in detail below. 
4.2 
RECRUITMENT 
There are ongoing NIH studies that see cancer survivors (e.g., 09-NR-0088), ME/CFSpatients 
(e.g., 16-N-0058), SLE and Sjogren’s patients, that will be some areas to recruit patients for this 
study. We also hope to enroll non-NIH participants. Flyers (Appendix 1a) may be posted on 
bulletin boards at NIH Clinical Center and in outpatient clinics’ waiting rooms. Public service 
announcements will also be distributed by the NIH CC PRPL/NINR 
Communications office through their Facebook/Twitter accounts, PRPL listserv, Research 
Match, and the NIH newsletter (Appendix 1b). Informational cards will also be made available 
to clinicians and potential participants (Appendix 1c and 1d). There will be no direct solicitation 
of employees or staff by supervisors or coworkers. It is anticipated that all 59 participants can be 
recruited in two to three years. Recruitment strategies have been re-evaluated and the study is 
open to the community as well as NIH patients. Potential participants will be notified about the 
study through social media posts, email, web postings, newsletters, power point presentations 
(Appendix 11), and educational flyers (Appendix 12). 
There is currently a shortage of ketamine supply in the NIH Clinical Center (CC) Pharmacy 
Department. This shortage will not impact the study recruitment but may lengthen the wait time 
to schedule the first study visit, and the overall duration of the study. We will work closely with 
the NIH CC Pharmacy Department in scheduling patient visits. 
Pre-screening will occur once potential participants inquire about the study. Upon receipt of the 
inquiry, the research team will perform a phone pre-screening using the pre-screening script 
(Appendix 2). Additionally, potential participants will be asked for their verbal/electronic 
written permission to allow the research team to access their NIH medical records, if available. 
4.3 
SCREENING 
The participant will be consented prior to any study procedures, including on-site screening. The 
Ability to Consent Assessment Team (ACAT) will be contacted if there is suspicion that the 
individual is unable to provide written informed consent. Appendix 3 will be used to determine 
eligibility. The screening visit will consist of the following: 
1. Fatigue VAS scale. Participants with fatigue VAS score of ≥ 50 mm (on a 0-100 
mm horizontal scale) will continue with the study. 
2. Revised Piper Fatigue Scale. Participants with fatigue ≥ six months total within a 
year will continue with the study. 
3. About 30 mL of blood will be drawn at the NIH CC laboratory. If results are 
available for the tests listed below and were collected less-than-a-week before the 
screening visit (with the exclusion of the pregnancy testing), and the participant 

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agrees to share with this study (unless indicated below), those values will instead be 
used. 
a. Complete blood count (CBC) w/diff 
b. C-reactive protein (CRP) 
c. Acute care panel 
d. Hepatic panel 
e. Thyroid panel (i.e. T3, T4, TSH) 
f. Viral markers panel 
g. Serum pregnancy testing (may be performed for individuals of child 
bearing potential if problems arise with obtaining urine pregnancy 
testing). 
4. Urine drug screen (for all potential participants). 
5. Alcohol screen by either breath test or using other biologic samples (e.g., urine). 
6. Pregnancy testing (for individuals of childbearing potential). 
7. A comprehensive clinical assessment by a Licensed Independent Practitioner (LIP), 
serving as Associate Investigator of the study. The assessment will take 
approximately one hour and will include measurement of vital signs (including 
orthostatic blood pressures, anthropometric measurement [waist- to-hip ratio], a 
detailed medical/social/surgical/psychiatric history, medication review, and 
physical examination. If a comprehensive clinical assessment by an LIP, who 
serves as AI of this study, has been done within a month by the referring NIH 
studies (e.g., CFS phenotyping protocol), this procedure will not be repeated in this 
study during screening. 
8. A review of clinical documentation detailing that the participant has completed 
treatment (for cancer survivors) or clinical documentation of diagnosis ME/CFS, 
CFS, chronic fatigue, fibromyalgia), or clinical documentation of diagnosis of SLE 
or Sjogren’s disease. 
4.4 
SCREEN FAILURES 
Screen failures are defined as participants who consent to participate in the clinical study but are 
not subsequently entered in the study. A minimal set of screen failure information is required to 
ensure transparent reporting of screen failure participants, to meet the Consolidated Standards of 
Reporting Trials (CONSORT) publishing requirements and to respond to queries from regulatory 
authorities. Minimal information includes demography, screen failure details, eligibility criteria, 
and any serious adverse event (SAE). 
Individuals who do not meet the criteria for participation in this study (screen failure), for 
example because of a lab value that is out of range may be rescreened at least 6 weeks after 
returning to their primary treating team. Rescreened participants should be reconsented and will 
be assigned the same participant number used in the initial screening. 
4.5 
STUDY PROCEDURES 
A copy of the study procedures, including study visits, will be provided to study participants 
(Appendix 9 & 10). To ensure full clinical support at the NIH Clinical Center, study visits will 
be pre-planned to ensure that the participants’ NIH out patient study visits will not fall on the 
weekends or federal holidays. 

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The procedures mentioned below will be undertaken at each of the study visits (see Appendix 4 
for Schedule of Events). A previous report confirms the diurnal variability of fatigue, where 
evening fatigue was observed to be significantly higher than morning fatigue (Dhruva et al., 
2013). To account for this variation, all patient-reported outcomes will be collected before the 
evening time (i.e., before 6pm). 
An overview of the study design and procedures is as follows: 
 
 
4.5.1 Period 1 
a) Baseline: A physical examination and review of medical history will be done by LIP and 
optional safety lab bloodwork, including CBC with Diff and Acute Care Panel, may be drawn, if 
available lab results are more than a week (7 days) from the infusion visit. Study questionnaires 
will be completed along with neurocognitive tests, skeletal muscle strength and EEfRT. The 
physical activity monitor will be given to participants at the end of the visit. 
b) Time point #1 (infusion visit): A urine drug and pregnancy test and alcohol screen (with 
breath test or using other biologic samples) will be administered prior to initiation of infusion. 
There are optional CBC with Diff and Acute Care panel blood draws, if results from screening 
labs are more than a week (7 days) from Timepoint 1. Research labs will be drawn throughout 
the infusion visit. The blinded solutions will be infused through intravenous tubing peripherally 
or using central ports. Participants will be observed for as long as four hours after study drug 
infusion (ketamine and active comparator) for cardiorespiratory status and psychosomatic 
symptoms. After about four hours of observation post infusion, the participant will complete 
study questionnaires and will be asked if they feel they received ketamine or the active 
comparator. Participants will be given instructions on their recovery after the drug infusion 
(Appendix 5). 

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c) Time point #2: A follow-up phone call will be made within ± 24 hours after Period 1 study 
drug administration. Participants will be asked about side effects to the study infusion and they 
will also be reminded to complete the Fatigue VAS. 
d) Time point #3: Participants will have an NIH outpatient visit within ± three business days 
after Period 1 study drug administration. Study questionnaires will be completed along with 
neurocognitive tests, skeletal muscle strength, EEfRT, physical exam, and blood draw for 
research blood. Participants will be asked about side effects to the study infusion. 
e) Time point #4: Participants will have an NIH outpatient visit within ± seven business days 
after Period 1 study drug administration. Study questionnaires will be completed along with 
neurocognitive tests, skeletal muscle strength, EEfRT, physical exam, and blood draw for 
research blood and safety labs (CBC with Diff and Acute Care Panel). Participants will be asked 
about side effects to the study infusion. 
f) Time point #5: Participants will have an NIH outpatient visit within ± 14 business days after 
Period 1 study drug administration. Study questionnaires will be completed along with a brief 
physical exam and a clinical safety labs (CBC with Diff and Acute Care Panel) may be drawn if 
available lab results are more than a week (7 days) from the infusion visit. Participants will be 
asked about side effects to the study infusion. 
4.5.2 Period 2 
a) Time point #6 (infusion visit): A urine drug and pregnancy test and alcohol screen (with 
breath test or using other biologic samples) will be administered prior to initiation of infusion. 
Research blood will be collected throughout the infusion visit. For the cross- over phase, the 
blinded study drug (ketamine or active comparator) will be administered through intravenous 
tubing peripherally or using central ports over a period of about 40 minutes. Similar to time point 
#1, participants will be observed for as long as four hours after study drug infusion (ketamine 
and active comparator) for cardiorespiratory status and psychosomatic symptoms. After about 
four hours of observation post infusion, the participant will complete study questionnaires and 
will be asked if they feel they received ketamine or active comparator. 
b) Time point #7: A follow-up phone call will be made within ± 24 hours after study drug 
administration. Participants will be asked about side effects to the study infusion and they will 
also be reminded to complete the Fatigue VAS. 
c) Time point #8: Participants will have an NIH outpatient visit within ± three business days after 
study drug administration. The study questionnaires will be completed together with the 
neurocognitive tests, skeletal muscle strength, EEfRT, physical exam, and a research blood draw. 
Participants will be asked about side effects to the study infusion. 
d) Time point #9: Participants will have an NIH outpatient visit within ± seven business days 
after study drug administration. The study questionnaires will be completed together with the 
neurocognitive tests, skeletal muscle strength, EEfRT, physical exam, and blood draw for 
research blood and safety labs (CBC with Diff and Acute Care Panel). Participants will be asked 
about side effects to the study infusion. 
e) Time point #10: A follow-up phone call will be made within ± 14 business days after Period 2 
study drug administration. Side effects to the study infusion will be asked and the participants 

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will be reminded to complete the Fatigue VAS. Participants will return the physical activity 
monitors by mail. 
The blinded study drug (ketamine or active comparator) will be administered by an Advanced 
Cardiac Life Support (ACLS)-certified, NIH credentialed Clinical Center nurse (all nurses 
staffed in the Procedure Service Unit of the Clinical Center, where thestudy drug will be 
administered, are required to maintain an active ACLS certification).The rest of the study 
outcomes will be administered by members of the research team. Participants will be monitored 
by research staff present with them at each study time point (i.e., nurse, physicians, and other 
members of the research team). If at any time during the study, a participant becomes distressed 
or an emergency situation develops, the research team staff will follow the NIH CC emergency 
procedures. 
4.6 
STUDY EVALUATIONS 
All questionnaires can be completed by paper-and-pencil and/or directly through password- 
protected web-based interfaces that can administer both static forms and computer adaptive test 
(CAT) versions of the questionnaires. These secure web-based interfaces 
(https://ctdb.nichd.nih.gov/ and https://cdrns.nih.gov/) are developed and maintained in 
accordance with NIH guidelines by the National Institute of Child Health and Human 
Development (NICHD) and Center of Informational Technology. Recent studies have shown 
equivalence in responses between questionnaires answered in electronic and paper/pen formats 
(Abernethy et al., 2008; Ring et al., 2008). 
Descriptions of all study evaluations are below. 
4.6.1 Fatigue Assessment: 
a) FACIT-Fatigue (FACIT-F) subscale is a 13-item questionnaire that explores fatigue symptoms 
in various populations, including cancer patients and healthy participants. This questionnaire has 
shown good test-retest reliability (r = 0.90), internal consistency (α = 0.93 and 0.95) on initial 
and test-retest administration suggesting that it can be administered as an independent, 
unidimensional measure of fatigue (Yellen et al., 1997) (~2 minutes). 
b) Patient Reported Outcome Measurement Information System (PROMIS) questionnaires are 
developed from more than 1000 datasets from multiple disease populations including cancer, 
heart disease, rheumatoid and osteoarthritis, psychiatric conditions, spinal cord injury, and 
chronic obstructive pulmonary disease. Fatigue, sleep disturbances, depression, and anxiety will 
be administered by CAT. Initial psychometric properties showed internal consistency reliability 
coefficient of 0.81 (Hays et al., 2009). These tests will be administered by short form if CAT 
version is unavailable (~5 minutes). 
c) The Fatigue VAS is a 0-100 mm scale. This is widely used to assess fatigue in patients with 
chronic illness (Segal et al., 2008) (<1 minute). 
4.6.1.1 DEPRESSIVE SYMPTOMS ASSESSMENT 
a) Hamilton Depression (HAM-D) utilizes a 21-item, clinician-rated paper questionnaire that 
explores severity of depressive symptoms of the participants in the past week prior to the 
interview using a Likert scale (0 = none to higher number from 2 to 4 = increasing intensity). 
When compared to other depression scales, the internal reliability of HAM- D is reported at 
>0.70 with Pearson’s r ranging from 0.82 to 0.98. Retest reliability ranged from0.81 to 0.98; and 

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it was more sensitive to change than the Beck Depression Scale and the Zung Self-Rating 
Depression Scale. The total HAM-D score for the 21- item version is obtained by adding the 
scores from items 1 to 17, as previously described (Sharp, 2015). The predefined cut off score 
for depression is 15 in a cancer study (Lydiatt et al., 2008), with higher scores indicating higher 
symptoms of depression. Individuals with higher HAM-D scores (scores above 17) indicating 
higher symptoms of depression will be referred to a NIH- credentialed psychiatrist for full 
evaluation. The HAM-D has been utilized to delineate depressive symptoms in cancer- related 
fatigue studies (Moss et al., 2006; Zhou et al., 2005) (~5 minutes). 
4.6.1.2 ASSESSMENT OF COGNITIVE AND PHYSICAL FUNCTIONING 
a) Massachusetts General Hospital Cognitive and Physical Functioning Questionnaire (MGH-
CPFQ) is a brief seven item self-report inventory to assess rates of significant cognitive 
symptoms, sleepiness, and fatigue in several clinical populations (Fava et al., 2006) (~2 
minutes). 
4.6.1.3 NEUROCOGNITIVE FUNCTION ASSESSMENT 
a) The NIH Toolbox neurocognitive performance tests will use the Dimensional Change Card 
Sort Test, the List Sorting Working Memory Test and the Pattern Comparison Processing Speed 
Test (~15minutes). 
4.6.1.4 SKELETAL MUSCLE STRENGTH ASSESSMENT 
a) Hand Dynamometry – Grip strength will be measured on both hands in a neutral position of 
the arm, forearm, and wrist. Two consecutive attempts at one-minute intervals will be measured 
in kilograms with the hand-held dynamometer. Each attempt will require a participant to exert a 
maximum possible grip force for about five seconds. After a minute of rest, the third attempt will 
measure fatigue resistance by asking the participant to hold the maximum hand grip as long as 
able until the 50% maximum grip force (based on the highest maximum hand grip force obtained 
from the first two attempts) is reached, which is often reached in less than a minute. The test will 
be repeated on the other hand. The same dynamometer will be used for all participants (~15 
minutes). 
4.6.1.5 PHYSICAL ACTIVITY ASSESSMENT: 
a) Physical activity will be measured daily while participating in the study using a portable 
physical activity monitor that the participant will wear in their own environment. The monitor 
can be worn by the participants on their wrists. Data can be extracted during their study visits. 
(~wear for duration of study participation). 
4.6.1.6 MOTIVATION ASSESSMENT 
a) The Effort-Expenditure for Rewards Task (EEfRT) is a multi-trial game in which participants 
are offered a choice between two task difficulty levels for a reward. The task begins with a one-
second fixation cross, followed by a five- second choice period in which the participant is 
informed of the probability of “winning” if the task is completed successfully. Next, the 
participant either completes 30 button presses in seven seconds with the dominant hand if they 
have chosen the easy task, or one- hundred button presses in 21 seconds using the non-dominant 
hand if they have chosen the hard task. Next, the participant receives feedback on whether they 
completed the task successfully or not. Finally, the participant learns if they have “won,” based 
upon the probability of winning and the successful completion of the task. This task will evaluate 

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the theory of value-based decision making in the pathophysiology of fatigue by looking for 
associations in reward and measures of fatigue and motivation in our participants (~20 minutes). 
4.6.1.7 BIOLOGIC SAMPLES (PERIPHERAL BLOOD) 
Blood will also be drawn for clinical (e.g., CBC and TSH) and research purposes during all NIH 
clinic visits and will comply with the limits dictated by the NIH ClinicalCenter Medical 
Administrative Series (MAS) policy on blood draws for research purposes (policy 95-9). No 
more than a total of 330 mL of blood will be drawn over thecourse of the study. 
a) Approximately 30 mL of blood will be drawn from participants at screening (e.g., CBC, TSH) 
to determine eligibility. 
b) Research blood (approximately 90 mL per infusion visit and approximately 30 mL for the 
outpatient study visits) will be drawn using PAXgene tube and cell preparation tubes to measure 
changes either in gene expression or protein levels of pro- inflammatory markers (e.g., 
lymphotoxin, IFNγ, TNFα) typically seen in fatigue, neurometabolites (e.g., brain derived 
neurotrophic factor [BDNF], glutamatergic/ dopaminergic markers), and mitochondrial markers 
(e.g., metabotropic glutamate receptors). Mitochondrial markers will be evaluated prior to initial 
infusion, prior to discharge after the infusion, and during all in person follow-up outpatient visits. 
4.6.1.8 KETAMINE ADMINISTRATION 
Participants will be administered ketamine (0.5 mg/kg) on either one of the drug infusion study 
visits. The administration will be by intravenous injection given through an intravenous line over 
40 minutes. Participants will be observed for potential side- effects to the study drug. 
4.6.1.9 ACTIVE COMPARATOR ADMINISTRATION 
To optimize blinding, participants will receive midazolam (0.045 mg/kg) as the active 
comparator, infused through peripheral IV over 40 minutes on either one of the 
drug infusion study visits. This specific dose of midazolam was considered equipotent to 
0.5 mg/kg of ketamine, based on a previous study (Murrough et al., 2013). Midazolam was 
selected based on a report describing its similar pharmacokinetic characteristic as ketamine: fast 
onset of action and short elimination half-life (Kanto, 1985). In addition, midazolam mimics 
ketamine’s nonspecific behavioral effects (e.g., sedation, disorientation [Gross et al., 1985]). 
However, the anti-depressant effect of metamine remains distinct when compared with an active 
placebo such as midazolam (Murrough et al., 2013) or an inactive placebo (Zarate et al., 2006). 
4.6.1.10  RANDOMIZATION 
Participants will be randomized prior to starting Period 1 of the study. An independent 
statistician will use a table of random numbers to determine ketamine or active comparator 
assignment of participants. This is done by choosing a series of randomized numbers 
corresponding to the number of participants and dividing the series into blocks of four. In each 
block of four, the two highest would receive ketamine and the two lowest would receive the 
active comparator. The randomization results will be relayed to the NIH CC Pharmacy before 
enrolling participants into the study, without divulging the randomization results to the study 
team. All study participants will be asked a randomization survey (Appendix 7a) after 
completing the two periods (ketamine and active comparator), to determine whether the 
participants felt they received ketamine or active comparator. Study staff present during the 

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infusions will also complete the randomization survey (Appendix 7b) to determine what drug 
they believe the participants received. This information will be saved with the study outcomes in 
a secured server and will enable the research team to determine if the study was indeed double-
blinded. 
4.6.2 End of participation 
Participation will end after time point #10. Participants will remain under the care of their own 
health care providers or referring team during study participation. Clinically relevant data 
obtained during participation will be shared with participants and their health care providers, 
with written permission from the participants. 
5 
MANAGEMENT OF DATA AND SAMPLES 
5.1 
STORAGE 
Blood samples will be stored in secured freezers in the NINR Symptom Management Branch 
until they are ready for batch analysis. Participants’ names and identifying information will be 
removed and the samples will be assigned codes. The key to the code will be kept in a separate, 
secure area. Participants will be consented to allow additional analyses of remaining blood 
samples collected from this study to be used for future experiments. 
5.2 
DATA AND SAMPLE SHARING PLAN 
Research data including results from outcome measures and location of biologic samples will be 
stored in the NINR-managed databases. These research data can only be accessed in the NIH 
password-protected websites by study investigators using a two- tiered security process both 
requiring entry of specific NIH username and password. 
Data and samples may be shared with collaborating laboratories at NIH, especially from 
referring teams (e.g., CFS phenotyping protocols) or with outside NIH collaborators and/or 
submitted to NIH-designated repositories and databases if consent for sharing was obtained. 
Repositories receiving data and/or samples from this protocol may be open-access or restricted 
access. 
Samples and data will be stripped of identifiers and may be coded (“de-identified”) or unlinked 
from an identifying code (“anonymized”). When coded data is shared, the key to the code will 
not be provided to collaborators but will remain at NIH. Data and samples may be shared with 
investigators and institutions with an FWA or operating under the Declaration of Helsinki (DoH) 
and reported at the time of continuing review. 
Sharing with investigators without an FWA or not operating under the DoH will be submitted for 
prospective IRB approval. Submissions to NIH-sponsored or supported databases and 
repositories will be reported at the time of Continuing Review. 
Submission to non-NIH sponsored or supported databases and repositories will be submitted for 
prospective IRB approval. 
Required approvals from the collaborating institution will be obtained and materials will be 
shipped in accordance with NIH and federal regulations. 
5.3 
RECORDS RETENTION 

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We will actively protect confidentiality of the participants and the data at each step. All medical 
records and participant data will be kept confidential and will only be reviewed by the 
participating investigators. Data will be stored using assigned codes and will be kept in 
password-protected computers held at the NINR. Only study investigators will have access to the 
study data. The participants’ names will not appear on any of the data forms. Participants’ 
personal information will be kept as private as possible. 
However, records can be inspected by organizations for quality assurance and data analysis. 
These include the members of the IRB and the NINR Quality Assurance /Quality Control 
Monitors. Participants’ health information will only be disclosed for the purposes of this research 
study. 
5.4 
CERTIFICATE OF CONFIDENTIALITY 
To further protect the privacy of study participants, the Secretary, Health and Human Services 
(HHS), has issued a Certificate of Confidentiality (CoC) to all researchers engaged in 
biomedical, behavioral, clinical or other human subjects research funded wholly or in part by the 
federal government. Recipients of NIH funding for human subjects research are required to 
protect identifiable research information from forced disclosure per the terms of the NIH Policy 
(see https://humansubjects.nih.gov/coc/index). As set forth in 45 CFR Part 
75.303(a) and NIHGPS Chapter 8.3, recipients conducting NIH-supported research covered by 
this Policy are required to establish and maintain effective internal controls (e.g., policies and 
procedures) that provide reasonable assurance that the protocol is managed in compliance with 
Federal statutes, and regulations. It is the NIH policy that investigators and others who have 
access to research records will not disclose identifying information except when the participant 
consents or in certain instances when federal, state, or local law or regulation requires disclosure. 
NIH expects investigators to inform research participants of the protections and the limits to 
protections provided by a Certificate issued by this Policy. 
6 
ADDITIONAL CONSIDERATIONS 
6.1 
RESEARCH WITH INVESTIGATIONAL DRUGS OR DEVICES 
This study has been reviewed by the US FDA and was deemed Investigational New Drug (IND) 
exempt. The FDA letter is attached in Appendix 8. 
7 
RISKS AND DISCOMFORTS 
The anticipated risks, discomforts and inconveniences of this protocol are those associated with 
the cognitive tasks, questionnaires and interviews, depression assessment, hand-grip strength 
testing, phlebotomy, and the ketamine and midazolam infusions. 
a) Cognitive Tasks, Motivation Tasks, Questionnaires and Interviews: Responding to 
questionnaires and tasks entail no medical risk. Some participants may be uncomfortable or 
anxious answering questions about their illness. Participants do not have to answer any question 
or perform tasks that make them uncomfortable and can stop the study at any time. 
b) Depression Assessment: Results of this assessment that may uncover real-time signs and 
symptoms of depression will trigger an immediate consultation with the NIMH consult service. 
Immediate inpatient hospitalization for psychiatric diagnosis will be guided by the findings of 
the NIMH consult service. Results of the consultation will be discussed with the participant and 

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with his/her consent, the health care providers will be informed. Referral with the NIMH clinical 
consult team will be arranged. 
c) Skeletal Muscle Strength Testing: Minimal discomfort might be experienced during skeletal 
strength tests (e.g., hand muscle soreness) which often lasts less than 5 minutes. 
d) Phlebotomy: Needle punctures for drawing blood may cause pain and carries a small risk of 
bleeding, bruising, discomfort and/or infection at the injection site, or temporary dizziness. The 
participants will be monitored for about 15 minutes after their blood drawing to assess and treat 
any complication related to the procedure. 
e) Ketamine: This is a general anesthetic for human and veterinary use. Ketamine has been 
administered as an anesthetic to several million adults and children over the last few decades and 
has a good safety profile. Ketamine exerts sympathomimetic activity and may produce mild to 
moderate increases in blood pressure, heart rate, and cardiac output. Participants may also 
experience central nervous system (CNS) effects such as hallucinations, scary thoughts, 
paranoia, or sedation. Since ketamine is given by infusion over 40 minutes, the likelihood for 
CNS effects to occur is reduced. If they occur, they often last for an hour after administration. 
For safety purposes, the study design used in this protocol is the same design used in the original 
NIMH study (Zarate et al., 2006). That study observed that adverse reactions from ketamine 
(e.g., perceptual disturbances, confusion, elevations in blood pressure, euphoria, dizziness, and 
increased libido) ceased within 80 minutes after the ketamine infusion and none of these adverse 
reactions persisted beyond 110 minutes. Ketamine is generally considered unsafe for use during 
pregnancy and breast-feeding. Please see section 1f of the protocol introduction for further 
discussion on the safety of ketamine. 
f) Midazolam: This is widely used, short-acting benzodiazepine and anesthetic agent used in 
adult and pediatric populations. It will be used as the active comparator in this study. A recent 
study (Murrough et al., 2013) reported that up to 4 hours after midazolam infusion, the most 
common adverse reactions include generalized malaise, dizziness, headache, restlessness, nausea 
or vomiting, dry mouth, decreased energy, and poor coordination. There were drops in blood 
pressure noted, but it resolved 240 minutes after infusion. Participants will be closely monitored 
during infusion and during the entire study. Management of side effects and emergency plan to 
handle adverse events are discussed below. Because of the sedative effect of ketamine and 
midazolam, participants are required to have another adult individual drive them home. The 
research team will provide transportation for study participants without a means of 
transportation, to take the participant with an accompanying responsible adult home after the 
infusion visits. 
g) Physical Activity Monitor: Minimal medical risk. Participants will be informed that they may 
refuse to perform a test or to stop a test at any time and for any reason. 
h) Based on the insert packages for both agents, aside from the adverse reactions mentioned 
above, these common adverse reactions from both the study drugs are also expected: Sore throat, 
eye pain, bad taste in mouth, increase in saliva and/or drooling, mood changes, confusion, 
unusual headache, tingling or “pins and needles”, itching or rash, weakness. 
8 
SUBJECT SAFETY MONITORING 
8.1 
STUDY DRUG ADMINISTRATION MONITORING: 

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Infusion, patient monitoring, and patient discharge will comply with the criteria and procedures 
dictated by NIH Clinical Center Medical Administrative Series (MAS) policy on administration 
of sedation (policy 92-9). Participants are closely monitored (continuously with telemetry, blood 
pressure, respiration, oxygen saturation) for potential study drug reactions during the 40-minute 
infusion of ketamine or midazolam, and additional 40 minutes post infusion. Participants will 
continue to be monitored (blood pressure, respiration, heart rate, oxygen saturation) for a 
maximum of 4 hours. Participants will be discontinued from the study if they develop manic, 
psychotic, or other symptoms that require treatment with medications. 
A clinician with advanced medical training (i.e., physician or nurse practitioner trained in ACLS 
and conscious sedation of anesthetic agents (i.e., ketamine and midazolam) will be present 
throughout the administration of study drugs so that adverse reactions can be evaluated and 
treated promptly. Medications such as flumazenil would be given at a clinically indicated dosing 
regimen if a patient were to experience serious anxiety, agitation, or other untoward effects. 
Anti-nausea medications may also be given for other study drug side effects. A fully equipped 
emergency medical cart is located nearby in the unlikely case of an untoward reaction or 
development of another health- related problem. Some evidence suggests that excessive noise 
and stimulation during recovery from sedation might be associated with emergence reactions (or 
psychotomimetic effects). Thus, the study drugs will be administered in a well-monitored 
location with muted lighting, noise, and physical contact. Prior to discharge, participants will be 
evaluated and confirmed to meet discharge criteria as described in MAS policy 92- 9. 
Adverse event assessments will be conducted during each study time point. For events that occur 
at the NIH and require immediate medical attention, procedures will be instituted at NIH or at a 
local hospital Emergency Room as deemed appropriate. With the patient’s consent, their 
treatment teams and healthcare providers will also be informed. 
Participants may withdraw from this study for any reason at any time without penalty or 
prohibition from enrolling in other NIH protocols for which they are eligible. Participants who, 
in the opinion of the PI and in consultation with the MAI, have an event that would make it 
unsafe to continue participation may skip or delay measurement of a specific study procedure, or 
be withdrawn from the study. 
Reasons for withdrawing a participant from the study at any time include: 
a) Clinically significant abnormal lab tests (e.g., absolute neutrophil count <1.5Kcells/µL, 
platelet 
<75K cells/µL, hemoglobin <9 g/dL). 
b) PI’s decision that it would be unsafe for the individual to continue participation(decision made 
in consultation with the MAI). 
c) Withdrawal of consent and/or participant decision. 
d) Psychotic, manic, or other symptoms that require pharmacological treatment. Data Collection 
of Adverse Events  
Not clinically significant and expected mild to moderate symptoms as listed in the risk section 
and safety labs will not be collected as AEs. 
9 
OUTCOME MEASURES 

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9.1 
PRIMARY OUTCOME MEASURES 
The primary outcome measure of the study is the percentage change in self-reported fatigue VAS 
score before and 3 days after receiving ketamine or active comparator. A 20% change in fatigue 
VAS score 3 days after ketamine treatment will be considered indication of efficacy in this study. 
9.2 
SECONDARY OUTCOME MEASURES 
The secondary outcomes of this study include: symptoms, physical activity count, skeletal 
muscle strength, motivation score, cognitive function test scores, gene expression or protein 
levels of pro- inflammatory markers (e.g., lymphotoxin, IFNγ, TNFα), neurometabolite (e.g., 
BDNF) levels, and mitochondrial markers (e.g., metabotropic glutamate receptors) before and 
after a dose of ketamine or active comparator at multiple time points. 
Missing data from either primary or secondary outcomes will not be used in the data analysis for 
that particular variable but will be included in the discussion of results. 
10 
STATISTICAL ANALYSIS 
10.1 ANALYSIS OF DATA/ STUDY OUTCOMES 
This is a double-blind, active comparator-controlled, cross-over study. The primary outcome 
measure will be the percentage change in fatigue scores (the difference between day 3 score 
minus the baseline score, divided by the baseline score) before and at 3rd day after a single 
intravenous dose of ketamine or active comparator. 
All statistical analysis will be conducted using available statistical software (e.g., SPSS and/or 
SAS). Significance will be evaluated at p<.05, two-tailed, unless otherwise stated. The 
Kolmogorov-Smirnov test will be used to examine whether the distribution of the outcome 
measure is normal. If the data are not normally distributed, transformations will be used to 
improve the fit. Carryover effect will be tested between AB vs. BA groups to see if there’s any 
statistical difference between the two groups. The difference of primary outcome (mean VAS 
percent changes) of ketamine (μA) and active comparator (μB) will be tested between the AB 
and BA sequence arms. If there’s a carryover effect, then each phase should be tested separately. 
10.2 ANALYSIS OF SECONDARY ENDPOINTS 
Secondary analysis will also examine the percentage change in fatigue symptoms, determined by 
comparing fatigue VAS scores prior to ketamine infusion and multiple time points after ketamine 
infusion. A linear mixed model with restricted maximum likelihood estimation will be used to 
examine changes in fatigue symptoms over the course of the trial where all participants with at 
least a pre-infusion and one post-infusion measure will be included. Within-subjects factors will 
include time with pre-infusion and all other points. The interaction between time and ketamine 
treatment will be included along with the fixed intercept. The random intercept and a random 
effect for subject will be included if they are a significant addition to the model. 
The effect of ketamine on VAS will also be tested by the binomial response outcome determined 
at 3-day post infusions. Clinical response will be determined by the proportion of change in VAS 
from pre-treatment to 3-day post treatment. Participants achieving 20% reduction or more 
between pre-infusion and day 3 will be considered responders. The proportion of responders in 
each clinical population will be compared using McNemar’s Test. Symptom evaluation will be 
conducted at the end of each study period (Periods 1 and 2) to determine who experienced a 

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clinical response. As exploratory analyses, other methods to determine efficacy, such as a 20-
point difference from pre- infusion to 3-day post study drug infusion and number of participants 
reaching 40- point mark in the fatigue VAS post study drug infusion will be investigated. Group 
comparisons of primary and secondary objectives will not be done using raw scores, but by the 
percentage change of each variable, pre and post treatment. 
The scores and levels of the other secondary outcomes (symptoms, physical activity count, 
skeletal muscle strength [e.g., maximum voluntary contractions], motivation score, cognitive 
function test scores, changes in gene expression or protein levels of pro- inflammatory markers, 
neurometabolite [e.g., BDNF] levels, and mitochondrial markers (e.g., glutamate receptor 4) will 
be compared using linear mixed model from prior, during, and after ketamine or active 
comparator infusion. The significance level may be adjusted using a Bonferroni approach based 
on the number of secondary measures examined with the linear mixed model. 
10.3 POWER ANALYSIS 
The primary aim of the study is to determine the effect of a single intravenous dose of ketamine 
(0.5 mg/kg) in reducing clinically significant fatigue in individuals with chronic illness, not on 
specific clinical populations. The anti-fatigue effect of ketamine will not be examined by clinical 
subgroup, but as an entire fatigue cohort with chronic illness. 
Since there is no previous ketamine study that used mixed model analysis measuring fatigue or 
other behaviors using the VAS, we estimated our sample size using a simple AB/BA crossover 
design, ignoring any covariates. 
The sample size estimate for the primary outcome is estimated by McNemar’s exact conditional 
test. If we estimate the discordant subject ratio to be 3:1, for example, the portions for response 
discordant subjects to be 0.45 for p10 (success for ketamine, failure for active comparator) and 
0.15 for p01 (failure for ketamine, success for active comparator), at significance level of 0.05, 
with expected power of 0.8, two-sided probability, the estimated number of participants required 
is 50. We anticipate a 15% drop out rate so the total number of participants to be recruited is 50 
+ 09 (drop out) = 59. We estimate to enroll 1-2 participants per month; hence, it will take about 2 
– 3 years to accrue 59 participants. 
10.4 INTERIM ANALYSIS FOR EFFICACY 
We plan an interim analysis of the participants who completed the study to answer the primary 
aim of the study: to determine the effect of a single intravenous dose of ketamine (0.5 mg/kg) in 
reducing clinically significant fatigue in individuals with chronic illness. The results of the 
interim analysis will guide in the planning for the next phase of the unit’s research program. So, 
it is requested that the study be unblinded for the Primary Investigator and other investigative 
staff to make a decision to discontinue the trial due to superiority or to continue the trial. 
The primary endpoint used for this analysis is the percentage of change in Fatigue Visual Analog 
Scale (VAS) scores. Change in fatigue VAS scores will be through comparison of fatigue VAS 
scores collected at the first visit (Baseline) and the third visit (3 days after study drug infusion) 
during each treatment arm (Ketamine, active comparator). For each participant, the difference in 
fatigue VAS score during the ketamine as well as the active comparator arm will be calculated. 
A 20% decrease in the fatigue VAS score is the predetermined primary outcome will be 
considered the indication of efficacy in this analysis. The hypothesis that fatigue VAS Diff = 0 

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will be tested by means using appropriate methods such as the student’s paired t-test using 
appropriate normal approximations. A p-value < 0.05 will be considered statistically significant. 
Besides the normality assumption for the paired t-test, the analysis of objectives makes two main 
assumptions: absence of period effect and no treatment-period interactions. A test for period 
effect will be performed by comparing the mean differences of the fatigue VAS scores for the 
two treatments orders (under the hypothesis that the difference is equal to 0, no period effect). If 
there is evidence for a period effect (p-value < 0.05), the results will be adjusted. The interaction 
between treatment and period will be examined by comparing the average of the fatigue VAS 
scores within patient between the two treatments of the First and Second Periods. If the 
interaction between treatment and period is significant (p-value < 0.05) only the first period will 
be used. 
Missing data values will be handled by using multiple data imputations. If available, baseline 
measurements at the beginning of each treatment period (First and Second Periods) would be 
included as covariates in the analysis. Analysis will be performed using the available software 
SPSS Statistics (IBM, New York, USA) or SAS (SAS Institute Inc., Cary, NC, USA). 
11 
HUMAN SUBJECTS PROTECTION 
11.1 SUBJECT SELECTION 
Accrual will be equitable for this study. Participants will be included regardless of race and 
ethnicity. Both women and men will be enrolled in the study because fatigue is not a gender- 
specific experience. Participants with active psychotic symptoms will be excluded from this 
study. 
11.2 JUSTIFICATION FOR EXCLUSION OF CHILDREN 
Participants younger than 18 are excluded as fatigue in adults differs from that in children. In 
addition, there are no studies available showing consistent data on the dose, pharmacokinetics, 
antidepressant efficacy and safety of ketamine in children with IV infusion. 
11.3 JUSTIFICATION FOR EXCLUSION OF OTHER VULNERABLE SUBJECTS 
11.3.1 Pregnant and lactating individuals: 
Will be excluded. Ketamine is generally considered unsafe for use during pregnancy and breast- 
feeding. Study participants will be reminded of this risk and will be encouraged to use 
appropriate birth control methods. 
11.3.2 Impaired consent capacity: 
Will not be included. Individuals who are not capable of giving their own informed consent will 
not be eligible for this study since study procedures will require participants to understand verbal 
and written questions and instructions. If necessary, consultation to assess consent capacity will 
be obtained from the NIH Ability to Consent Assessment Team (301-496-9675). 
NIH staff: 
May be included. With no scientifically based reason to exclude NIH employees/staff, we can 
offer the opportunity for this group to participate in this study if interested and eligible. 
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Fatigue is a very common symptom of HIV+ patients even those who are optimally treated with 
highly active anti-retroviral treatment (Andersen et al., 2006). Furthermore, it has been shown 
that increased levels of pro-inflammatory cytokines are found in the central nervous system of 
HIV+ individuals (Tyor et al., 1992). To limit this variable, this study will exclude patients who 
are HIV+. Individuals who are not capable of giving their own informed consent will not be 
eligible for this study since study procedures will require participant to understand verbal and 
written questions and instructions. There is also strong evidence showing that ketamine, 
especially at higher doses can exacerbate psychotic symptoms. To avoid this, individuals with 
active psychotic symptoms are excluded from this study. 
11.4 JUSTIFICATION OF SENSITIVE PROCEDURES 
11.4.1 Active comparator arm: 
By using an active comparator, we will be able to expose the fewest participants to experimental 
conditions to know whether a rapid anti-fatigue response can be achieved with a single 
intravenous dose of ketamine. An active comparator condition would help us interpret study 
results limiting patient bias. 
11.4.2 Drug and alcohol-free period: 
Participants will be free of illicit drug and alcohol for two weeks prior to ketamine 
administration and during the study. This drug- and alcohol-free period was chosen to reduce the 
risk of a carry- over effect. Such a carry-over effect from previous treatments could make study 
results difficult to interpret. 
11.5 SAFEGUARDS FOR VULNERABLE POPULATIONS 
Participants with psychotic symptoms during screening will be referred immediately to the 
NIMH consult service. 
11.5.1 Requirement of a primary care provider for study participation: 
The study will not provide any clinical care and it will not diagnose any clinical conditions. Any 
study-related clinical findings will be relayed to the primary care provider for further evaluation 
and management. 
11.5.2 NIH staff: 
All participants will be asked if they are an employee of NIH during screening. NIH staff will 
not be solicited for participation but will not be excluded if they express the desire to enroll. 
Protections for employees and staff participating in this study include: 1) assuring that the 
participation or refusal to participate will have no effect, either beneficial or adverse, on the 
participant’s employment or position at NIH, 2) giving employees and staff who are interested in 
participating the “NIH Information Sheet on Employee Research Participation” prior to 
obtaining consent (see Appendix C from OHSRP SOP 14f), and 3) assuring that there will be no 
direct solicitation of employees or staff. Employees that participate in this protocol during work 
hours will be informed that they must obtain their supervisor’s permission and that per NIH 
guidelines they will not be paid (as described in section 23). 
To ensure that research staff are adequately trained to respect the privacy and confidentiality of 
NIH employees/staff, study investigators and staff are required to complete the CITI “Just in 
Time” Training: Vulnerable Subjects – Workers/Employees. In addition, all study investigators 

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and staff who have contact with study participants or who have access to medical records will be 
required to complete the mandatory “NIH Clinical Center Patient Confidentiality and Privacy” 
training at the start of their employment and annually thereafter. These trainings must be 
completed prior to study investigators and staff having contact with participants and/or 
personally identifiable information (PII). 
12 
ANTICIPATED BENEFIT 
This protocol offers no direct benefit from participation. Previous result from a placebo-
controlled study found that ketamine was effective in the short-term for treatment of major 
depressive disorder and bipolar disorders (Zarate et al., 2006). Findings from this proposed study 
may provide generalizable knowledge about developing new treatments for fatigue. 
13 
CONSENT DOCUMENTS AND PROCESS 
13.1 DESIGNATION OF THOSE OBTAINING CONSENT 
Study investigators designated as able to obtain consent, per the study personnel page, will 
obtain informed consent. Only associate investigators who are licensed independent 
professionals and credentialed to prescribe medications in the NIH Clinical Center (e.g., MDs, 
NPs) are designated to obtain informed consent for this study. All study investigators obtaining 
informed consent have completed the NIMH Human Subjects Protection Unit (HSPU) 
“Elements of Successful Informed Consent” training. 
13.2 CONSENT PROCEDURES 
All participants will receive a verbal explanation in terms suited to their comprehension of the 
purposes, procedures and potential risks of the study and of their rights as research participants. 
They will be informed that they may withdraw from the study at any time without prejudice to 
themselves. Participants will have the opportunity to carefully review the written consent form 
and ask questions regarding this study prior to signing. The PI will sign the consent form. The 
original consent form will be filed with NIH Medical Records. A copy of the completed signed 
consent will be provided to the participant. 
Consent Process by Telephone/NIH-Approved Telehealth Platform/iMED 
Informed consent will be obtained per the Clinical Center Policy and Communication Bulletin 
MAS policy M20-1 and IRB Policy 303. Subjects will be provided a copy of the consent for 
review. Subjects will be given ample time to read and review the consent before the appointment 
for the formal informed consent process and signing. Subjects will choose the location for the 
telephone/telehealth informed consent process, but the research team will highly encourage the 
subjects to choose a location that will private, quiet, and have the remote possibility of 
interruptions. Subjects will be contacted either by telephone or NIH-approved telehealth platform 
and the non-investigational nature of this trial, the associated assessment and test options, 
biological sample collection, and attendant risks and discomforts will be carefully explained to 
the subject. 
The subject will sign and date either the paper informed consent or use electronic signature (e- 
sign) using the CC approved platform or method (iMED). 
If electronic signatures will be used, the study team plans to use the CC approved platform, 
currently iMED consent, to obtain electronic signatures. The identity of participants will be 

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verified by asking them to state their full name and date of birth. The electronic signatures will 
be obtained “synchronously and in real-time” whether it is in-person, telephone, or telehealth 
consent. iMED consent will capture electronic signatures via the methods listed below: 
• Using a smartphone: participants will be registering their signatures using their 
finger. Participants will receive a secure link that leads them to secure “web 
pages” that will capture their electronic signatures using their finger. 
• Using a computer/tablet/laptop: participants will be registering their signatures 
using a computer mouse or their finger/stylus (for touch/stylus enabled tablets and 
computer screens). Participants will receive a secure link that leads them to secure 
“web pages” that will capture their electronic signatures using the aforementioned 
methods. 
Signed paper copies of consent will be returned via fax, mail, or secure e-mail to the consenting 
investigator to be signed and dated. A fully executed copy of the consent will be available in the 
participant’s research record. Consent forms signed using the CC approved electronic signature 
platform (iMED consent) will be archived and stored in the participant’s research record via an 
automatic secure electronic transmission immediately after all the required signatures are 
obtained. All participants will be provided with a copy of the fully executed consent (paper copy 
by mail or electronic copy by secure electronic methods based on participant’s preference). The 
informed consent process will be documented in the medical record, including the name of the 
interpreter. 
13.3 CONSENT DOCUMENTS 
The consent form contains all required elements. 
14 
DATA AND SAFETY MONITORING 
14.1 DATA AND SAFETY MONITOR 
 Data and safety will be monitored primarily by the NIMH-IRP Data and Safety Monitoring 
Board (DSMB). 
14.2 DATA AND SAFETY MONITORING PLAN 
The data and safety monitoring plan (DSMP) of the study includes monitoring by the NIMH-IRP 
DSMB. The DSMB will meet every 4-6 months to review study accrual and progress, adverse 
events related to the study, and safety and outcome data. Specific data elements required by the 
DSMB will be established at the first meeting the protocol is reviewed. The DSMB will have the 
authority to require changes in the study design, or to stop all or part of any study based on 
accumulating safety data. The PI will prepare a report to the DSMB on a semi-annual basis or as 
requested by the board. 
The PI will also be responsible for monitoring data and safety. Issues of participant recruitment, 
participant enrollment, data collection, data storage, and other safety events will be reported to 
the PI every week by the research staff. Involved associate investigators (AIs) will be informed 
about these issues on a quarterly established schedule. 
The medically responsible investigator has the authority to break the blind in the event of an 
emergency. Breaking of the blind, life threatening injury or death all require immediate reporting 
to the DSMB and the NINR Clinical Director. A written notification to the DSMB chair must 

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follow within 7 days. Specific requirements for data safety monitoring will abide by the 
document entitled “Standard Operating Procedures for the NIMH IRP Data and Safety 
Monitoring Board.” 
14.3 CRITERIA FOR STOPPING THE STUDY OR SUSPENDING ENROLLMENT OR PROCEDURES 
As no SAEs are anticipated, the study will temporarily cease enrolling new patients if any 
potentially related SAEs occur, until the PI and the IRB determine there is no risk to continuing. 
Other investigators will consult with the PI if they believe that such actions are warranted to 
protect the safety, welfare or rights of subjects or to protect data integrity. The PI and the NINR 
CD may also recommend temporarily suspending or closing enrollment or stopping the study at 
any time due to safety concerns or slow recruitment. 
15 
QUALITY ASSURANCE (QA) 
15.1 QUALITY ASSURANCE MONITOR 
The quality assurance of the study will be monitored by a QA monitor, who is independent of the 
study. 
15.2 QUALITY ASSURANCE PLAN 
This is a more than minimal risk study. Per the NINR quality assurance policy, this study will be 
monitored after enrollment of the first participant, annually thereafter, and at close-out. Ad hoc 
monitoring visits may be scheduled per the request of the study team, the independent monitor, 
or NINR Clinical Director. 
16 
REPORTING OF UNANTICIPATED PROBLEMS, ADVERSE EVENTS AND 
PROTOCOL DEVIATIONS 
The Principal Investigator is responsible for detecting, documenting, and reporting unanticipated 
problems, AEs, including SAEs and deviations in accordance with NIH Policy 801. Relatedness 
to the research of all SAEs will be determined by the PI in consultation with the NINR CD and 
the Medical Advisory Investigator for the study. 
17 
ALTERNATIVES TO PARTICIPATION 
There is no FDA-approved medication to treat fatigue. Aside from activity enhancement, 
National Comprehensive Cancer Network (NCCN) guidelines recommend psychosocial 
improvement, attention-restoring therapy, nutrition, and sleep to manage fatigue. NCCN further 
recommends the use of psychostimulants to manage fatigue once other causes (e.g., anemia, 
sleep disorders) are ruled out. The effectiveness of these recommended interventions has not 
been validated in large clinical trials. 
18 
PRIVACY 
All research activities will be conducted in as private a setting as possible. 
19 
CONFIDENTIALITY 
19.1 FOR RESEARCH DATA AND INVESTIGATOR MEDICAL RECORDS 
The information obtained from participants will be stored electronically and in paper files in a 
secure location at the NIH CC. Hard copies will be kept in double-locked storage. Electronic 

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records will be stored in password protected files on secure servers at the NIH. The information 
will be kept private. Only study staff will have access to the information. 
19.2 FOR STORED SAMPLES 
Blood samples will be dated (date of collection) and stored using codes that the research team 
will assign in locked NINR Laboratory of Symptom Management freezers until they are 
analyzed. Only study investigators will have access to the stored samples collected in this study. 
19.3 SPECIAL PRECAUTIONS 
Data will be kept in password-protected computers. Hard copy data/records are stored in a 
locked cabinet in a room in NIH CC that is locked when unoccupied. Research data including 
results from outcome measures and location of biologic samples will be stored in the Clinical 
Trials Database (CTDB) and Common Data Repository for Nursing Science (cdRNS). These 
web-based applications are compliant with NIH privacy and confidentiality requirements and 
policies. Only study investigators will have access to stored data collected in this study. De-
identified results from this study will be posted on clinicaltrials.gov. 
20 
CONFLICT OF INTEREST 
The independence of this study from any actual or perceived influence, such as by the 
pharmaceutical industry, is critical. Therefore, any actual conflict of interest of persons who have 
a role in the design, conduct, analysis, publication, or any aspect of this trial will be disclosed 
and managed. Furthermore, persons who have a perceived conflict of interest will be required to 
have such conflicts managed in a way that is appropriate to their participation in the design and 
conduct of this trial. The study leadership in conjunction with the NINR has established policies 
and procedures for all study group members to disclose all conflicts of interest and will establish 
a mechanism for the management of all reported dualities of interest. 
21 
TECHNOLOGY TRANSFER 
 There are no transfers or agreements to report. 
22 
RESEARCH AND TRAVEL COMPENSATION 
Participants will be compensated for research-related inconveniences. Compensation will be 
prorated for parts completed if participants do not complete the study. Payments will be sent 
after each visit. The schedule for procedure-based payments is below: 
**EEfRT: compensation up to $20 based on the participant’s EEfRT score per visit. 
Period 1 Baseline Visit 
Payment 
Blood draw 
$10 
Questionnaires (all) 
$20 
Cognitive/Psychological Testing (HAM- 
D, Hand Strength, NIH Toolbox) 
$20 
Total before EEfRT 
$50 
**EEfRT up to $20 
Up to $20 
Total with EEfRT 
$70 
Period 1 Infusion 1 
Payment 

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Blood Draw – Serial/Sampling through IV 
$100 
Heparin Lock insertion (per attempt) 
$10 
Questionnaire (VAS x 5, 6 questionnaires, 
randomization, AE assessment, HAMD) 
$40 
Total 
$150 
Period 1 Telephone call follow-up 
Payment 
Telephone Follow-up/Interview 
$10 
Period 1 Follow-up Visit (+/- 3 days) 
Payment 
Blood draw 
$10 
Questionnaires (all) 
$20 
Cognitive/Psychological Testing (HAM- 
D, Hand Strength, NIH Toolbox) 
$20 
Total before EEfRT 
$50 
 
**EEfRT up to $20 
 
Up to $20 
Total with EEfRT 
$70 
Period 1 Follow-up Visit (+/- 7 days) 
Payment 
Blood draw 
$10 
Questionnaires (all) 
$20 
Cognitive/Psychological Testing (HAM-D, 
Hand Strength, NIH Toolbox) 
$20 
Total before EEfRT 
$50 
 
**EEfRT up to $20 
 
Up to $20 
Total with EEfRT 
$70 
 
 
 
 
 
 
 
 
Period 2 Infusion 2 
Payment 
Blood Draw – Serial/Sampling through IV 
(research blood x 5) 
$100 
Heparin Lock insertion (IU per attempt) 
$10 
Questionnaire (VAS x 5, 6 questionnaires, 
randomization, AE assessment, HAMD) 
$40 
Total 
$150 
Period 2 Baseline Visit 
Payment 
(Optional) Safety Blood draw 
$10 
Questionnaires (all) 
$20 
 
 
Total up to  
$30 
 
 

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Period 2 Telephone call follow-up 
Payment 
Telephone Follow-up/Interview 
$10 
Period 2 Follow-up Visit (+/- 3 days) 
Payment 
Blood draw 
$10 
Questionnaires (all) 
$20 
Cognitive/Psychological Testing (HAM- 
D, Hand Strength, NIH Toolbox) 
$20 
Total before EEfRT 
$50 
**EEfRT up to $20 
Up to $20 
Total with EEfRT 
$70 
Period 2 Telephone call follow-up 
Payment 
Telephone Follow-up/Interview 
$10 
Total payment at the end of Period 2 = $340 
Total payment entire protocol = $710 
This study will offer reimbursement or payment for meals and other reimbursements, on a case- 
by-case basis, based on NIH guidelines. 
The amount of payment to research volunteers is guided by the National Institutes of Health 
policies. In general, participants are not paid for taking part in research studies at the National 
Institutes of Health. Payment will be sent after each visit. The Institute can pay or reimburse car 
mileage based on the Government reimbursement rate. There will not be prorated compensation. 
NIH employees and staff who participate during work hours must have permission from their 
supervisor. NIH employees or staff must either participate outside of work hours or take leave in 
order to receive transportation assistance. 
 
 

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23 
REFERENCES 
Aan Het Rot, M., Zarate Jr, C. A., Charney, D. S., & Mathew, S. J. (2012). Ketamine for 
depression: where do we go from here? Biological psychiatry, 72(7), 537-547. 
Abernethy, A. P., Herndon, J. E., Wheeler, J. L., Patwardhan, M., Shaw, H., Lyerly, H. K., & 
Weinfurt, K. (2008). Improving health care efficiency and quality using tabletpersonal computers 
to collect research‐quality, patient‐reported data. Health services research, 43(6), 1975-1991. 
Andersen, Å. B., Law, I., Ostrowski, S. R., Lebech, A. M., Høyer-Hansen, G., Højgaard,L., ... & 
Kjaer, A. (2006). Self-reported fatigue common among optimally treated HIV patients: no 
correlation with cerebral FDG-PET scanning abnormalities. Neuroimmunomodulation, 13(2), 
69-75. 
Arends, S., Meiners, P. M., Moerman, R. V., Kroese, F. G., Brouwer, E., Spijkervet, F. K., ... & 
Bootsma, H. (2017). Physical fatigue characterises patient experience of primary Sjögren's synd 
Arnold, L. M. (2008). Understanding fatigue in major depressive disorder and othermedical 
disorders. Psychosomatics, 49(3), 185-190. 
Bårdsen, K., Nilsen, M. M., Kvaløy, J. T., Norheim, K. B., Jonsson, G., & Omdal, R.(2016). 
Heat shock proteins and chronic fatigue in primary Sjögren’s syndrome.Innate immunity, 22(3), 
162- 167. 
Bayliss, K., Goodall, M., Chisholm, A., Fordham, B., Chew-Graham, C., Riste, L., ... 
&Wearden, A. (2014). Overcoming the barriers to the diagnosis and management of chronic 
fatigue syndrome/ME in primary care: a meta synthesis of qualitative studies. BMC family 
practice, 15(1), 44. 
Berman, R. M., Cappiello, A., Anand, A., Oren, D. A., Heninger, G. R., Charney, D. S.,& 
Krystal, J. H. (2000). Antidepressant effects of ketamine in depressed patients. Biological 
psychiatry, 47(4), 351-354. 
Bowman, S. J., Everett, C. C., O'dwyer, J. L., Emery, P., Pitzalis, C., Ng, W. F., ... & Hall, F. C. 
(2017). Randomized controlled trial of rituximab and cost‐effectiveness analysis in treating 
fatigue and oral dryness in primary Sjögren's syndrome. Arthritis& Rheumatology, 69(7), 1440- 
1450. 
Brække Norheim, K., Imgenberg-Kreuz, J., Jonsdottir, K., Janssen, E. A., Syvänen, A.C., 
Sandling, J. K., ... & Omdal, R. (2016). Epigenome-wide DNA methylation patterns associated 
with fatigue in primary Sjögren’s syndrome. Rheumatology, 55(6), 1074-1082. 
Bower, J. E., Ganz, P. A., Aziz, N., & Fahey, J. L. (2002). Fatigue and proinflammatorycytokine 
activity in breast cancer survivors. Psychosomatic medicine, 64(4), 604- 611. 
Brola, W., Ziomek, M., & Czernicki, J. (2007). Fatigue syndrome in chronic neurological 
disorders. Neurologia i neurochirurgia polska, 41(4), 340-349. 

41 
Abbreviated Title: Ketamine on Fatigue 
Version Date: 04/18/2024 
Interventional Template v7 06.14.2023 
 
Carlotto, A., Hogsett, V. L., Maiorini, E. M., Razulis, J. G., & Sonis, S. T. (2013). Theeconomic 
burden of toxicities associated with cancer treatment: review of the literature and analysis of 
nausea and vomiting, diarrhoea, oral mucositis and fatigue. Pharmacoeconomics, 31(9), 753- 
766. 
Carruthers, B. M., Jain, A. K., De Meirleir, K. L., Peterson, D. L., Klimas, N. G., Lerner, A. M., 
... & Sherkey, J. A. (2003). Myalgic encephalomyelitis/chronic fatiguesyndrome: clinical 
working case definition, diagnostic and treatment protocols. Journal of chronic fatigue syndrome, 
11(1), 7-115. 
Clayton, E. W. (2015). Beyond myalgic encephalomyelitis/chronic fatigue syndrome: anIOM 
report on redefining an illness. Jama, 313(11), 1101-1102. 
Dassouki, T., Benatti, F. B., Pinto, A. J., Roschel, H., Lima, F. R., Augusto, K., ... & deSá Pinto, 
A. L. (2017). Objectively measured physical activity and its influence onphysical capacity and 
clinical parameters in patients with primary Sjögren’s syndrome. Lupus, 26(7), 690-697. 
Dhruva, A., Aouizerat, B. E., Cooper, B., Paul, S. M., Dodd, M., West, C., ... & Merriman, J. D. 
(2013). Differences in morning and evening fatigue in oncology patients and their family 
caregivers. European Journal of Oncology Nursing, 17(6),841-848. 
Dittner, A. J., Wessely, S. C., & Brown, R. G. (2004). The assessment of fatigue: apractical 
guide for clinicians and researchers. Journal of psychosomatic research, 56(2), 157-170. 
Eckhardt, A. L., DeVon, H. A., Piano, M. R., Ryan, C. J., & Zerwic, J. J. (2014). Fatiguein the 
presence of coronary heart disease. Nursing research, 63(2), 83-93. 
Esliger, D. W., & Tremblay, M. S. (2006). Technical reliability assessment of three 
accelerometer models in a mechanical setup. Medicine and science in sports andexercise, 38(12), 
2173-2181. 
Fava, M., Graves, L. M., Benazzi, F., Scalia, M. J., Iosifescu, D. V., Alpert, J. E., & Papakostas, 
G. I. (2006). A cross-sectional study of the prevalence of cognitive andphysical symptoms during 
long-term antidepressant treatment. The Journal of clinical psychiatry, 67(11), 1754-1759. 
Feng, L. R., Fernández-Martínez, J. L., Zaal, K. J., Wolff, B. S., & Saligan, L. N. 
(2018).mGluR5 mediates post-radiotherapy fatigue development in cancer patients. Translational 
psychiatry, 8(1), 110. 
Fonseca, R., Bernardes, M., Terroso, G., de Sousa, M., & Figueiredo-Braga, M. (2014). Silent 
burdens in disease: fatigue and depression in SLE. Autoimmunediseases, 2014. 
Fossey, M., Libman, E., Bailes, S., Baltzan, M., Schondorf, R., Amsel, R., & Fichten, C. S. 
(2004). Sleep quality and psychological adjustment in chronic fatiguesyndrome. Journal of 
behavioral medicine, 27(6), 581-605. 

42 
Abbreviated Title: Ketamine on Fatigue 
Version Date: 04/18/2024 
Interventional Template v7 06.14.2023 
 
Fukuda, K., Straus, S. E., Hickie, I., Sharpe, M. C., Dobbins, J. G., & Komaroff, A. (1994). The 
chronic fatigue syndrome: a comprehensive approach to its definitionand study. Annals of 
internal medicine, 121(12), 953-959. 
Hann, D. M., Garovoy, N., Finkelstein, B., Jacobsen, P. B., Azzarello, L. M., & Fields, K. K. 
(1999). Fatigue and quality of life in breast cancer patients undergoing autologous stem cell 
transplantation: a longitudinal comparative study. Journal ofpain and symptom management, 
17(5), 311-319. 
Hays, R. D., Bjorner, J. B., Revicki, D. A., Spritzer, K. L., & Cella, D. (2009). Development of 
physical and mental health summary scores from the patient-reported outcomes measurement 
information system (PROMIS) global items. Quality of Life Research, 18(7), 873-880. 
Hewlett, S., Cockshott, Z., Byron, M., Kitchen, K., Tipler, S., Pope, D., & Hehir, M.(2005). 
Patients' perceptions of fatigue in rheumatoid arthritis: overwhelming, uncontrollable, ignored. 
Arthritis Care & Research, 53(5), 697-702. 
Iadarola, N. D., Niciu, M. J., Richards, E. M., Vande Voort, J. L., Ballard, E. D., Lundin, N. B., 
... & Zarate Jr, C. A. (2015). Ketamine and other N-methyl-D-aspartatereceptor antagonists in 
the treatment of depression: a perspective review. Therapeutic advances in chronic disease, 6(3), 
97-114. 
James, K., Al-Ali, S., Tarn, J., Cockell, S. J., Gillespie, C. S., Hindmarsh, V., ... & Price, E. 
(2015). A transcriptional signature of fatigue derived from patients with primarySjögren’s 
syndrome. PloS one, 10(12), e0143970. 
Jason, L. A., Benton, M. C., Valentine, L., Johnson, A., & Torres-Harding, S. (2008). The 
economic impact of ME/CFS: individual and societal costs. DynamicMedicine, 7(1), 6.  
Kamath, J., Feinn, R., & Winokur, A. (2012). Thyrotropin-releasing hormone as a treatment for 
cancer-related fatigue: a randomized controlled study. Supportive Carein Cancer, 20(8), 1745- 
1753. 
Kamath, J. (2012). Cancer-related fatigue, inflammation and thyrotropin-releasinghormone. 
Current aging science, 5(3), 195-202. 
Kanto, J. H. (1985). Midazolam: The First Water‐soluble Benzodiazepine;Pharmacology, 
Pharmacokinetics and Efficacy in Insomnia and Anesthesia. Pharmacotherapy: The Journal of 
Human Pharmacology and DrugTherapy, 5(3), 138-155. 
Katon, W., & Lin, E. (1991). Somatization: a spectrum of severity. The American journalof 
psychiatry, 148(1), 34. 
Kim, H. J., & Abraham, I. (2017). Measurement of fatigue: Comparison of the reliabilityand 
validity of single-item and short measures to a comprehensive measure. International journal of 
nursing studies, 65, 35-43. 

43 
Abbreviated Title: Ketamine on Fatigue 
Version Date: 04/18/2024 
Interventional Template v7 06.14.2023 
 
Klokkerud, M., Dagfinrud, H., Uhlig, T., Dager, T. N., Furunes, K. A., Klokkeide, Å., ...& 
Kjeken, I. (2018). Developing and testing a consensus-based core set of outcomemeasures for 
rehabilitation in musculoskeletal diseases. Scandinavian journal of rheumatology, 47(3), 225-
234. 
Koh, J. H., Kwok, S. K., Lee, J., & Park, S. H. (2017). Autonomic dysfunction in primary 
Sjogren’s syndrome: a prospective cohort analysis of 154 Korean patients. The Korean journal of 
internal medicine, 32(1), 165. 
Krupp, L. B., LaRocca, N. G., Muir-Nash, J., & Steinberg, A. D. (1989). The fatigueseverity 
scale: application to patients with multiple sclerosis and systemic lupus erythematosus. Archives 
of neurology, 46(10), 1121-1123. 
Krupp, L. B. (2003). Fatigue in multiple sclerosis: definition, pathophysiology andtreatment. 
CNS 332. Drugs, 17(4), 225-234. 
Lydiatt, W. M., Denman, D., McNeilly, D. P., Puumula, S. E., & Burke, W. J. (2008). A 
randomized, placebo-controlled trial of citalopram for the prevention of major depression during 
treatment for head and neck cancer. Archives of Otolaryngology–Head & Neck Surgery, 134(5), 
528-535. 
Minton, O., & Stone, P. (2008). A systematic review of the scales used for the measurement of 
cancer-related fatigue (CRF). Annals of oncology, 20(1), 17-25. 
Minnock, P., Kirwan, J., & Bresnihan, B. (2009). Fatigue is a reliable, sensitive and unique 
outcome measure in rheumatoid arthritis. Rheumatology, 48(12), 1533-1536. 
Morris, G., Anderson, G., & Maes, M. (2017). Hypothalamic-pituitary-adrenal hypofunction in 
myalgic encephalomyelitis (ME)/chronic fatigue syndrome (CFS) asa consequence of activated 
immune-inflammatory and oxidative and nitrosative pathways. Molecular neurobiology, 54(9), 
6806-6819. 
Moss, E. L., Simpson, J. S. A., Pelletier, G., & Forsyth, P. (2006). An open‐label study ofthe 
effects of bupropion SR on fatigue, depression and quality of life of mixed‐site cancer patients 
and their partners. Psycho‐Oncology: Journal of the Psychological, Social and Behavioral 
Dimensions of Cancer, 15(3), 259-267. 
Murrough, J. W., Iosifescu, D. V., Chang, L. C., Al Jurdi, R. K., Green, C. E., Perez, A. M., ... & 
Charney, D. S. (2013). Antidepressant efficacy of ketamine in treatment- resistant major 
depression: a two-site randomized controlled trial. American Journalof Psychiatry, 170(10), 
1134-1142. 
Ng, W. F., Stangroom, A. J., Davidson, A., Wilton, K., Mitchell, S., & Newton, J. L. (2012). 
Primary Sjögrens syndrome is associated with impaired autonomic responseto orthostasis and 
sympathetic failure. QJM: An International Journal of 
Medicine, 105(12), 1191-1199. 

44 
Abbreviated Title: Ketamine on Fatigue 
Version Date: 04/18/2024 
Interventional Template v7 06.14.2023 
 
Overman, C. L., Kool, M. B., Da Silva, J. A., & Geenen, R. (2016). The prevalence ofsevere 
fatigue in rheumatic diseases: an international study. Clinical rheumatology, 35(2), 409-415. 
Pawlikowska, T., Chalder, T., Hirsch, S. R., Wallace, P., Wright, D. J. M., & Wessely, S. C. 
(1994). Population based study of fatigue and psychologicaldistress. Bmj, 308(6931), 763- 766. 
Pihur, V., Datta, S., & Datta, S. (2011). Meta analysis of chronic fatigue syndromethrough 
integration of clinical, gene expression, SNP and proteomic data. Bioinformation, 6(3), 120. 
Portenoy, R. K., & Itri, L. M. (1999). Cancer-related fatigue: guidelines for evaluationand 
management. The oncologist, 4(1), 1-10. 
Prommer, E. E. (2012). Ketamine for pain: an update of uses in palliative care. Journal of 
palliative medicine, 15(4), 474-483. 
Ramsey-Goldman, R., & Rothrock, N. (2010). Fatigue in systemic lupus erythematosusand 
rheumatoid arthritis. PM&R, 2(5), 384-392. 
Ricci, J. A., Chee, E., Lorandeau, A. L., & Berger, J. (2007). Fatigue in the US workforce: 
prevalence and implications for lost productive work time. Journal ofOccupational and 
Environmental Medicine, 49(1), 1-10. 
Ring, A. E., Cheong, K. A., Watkins, C. L., Meddis, D., Cella, D., & Harper, P. G. (2008). A 
randomized study of electronic diary versus paper and pencil collection ofpatient-reported 
outcomes in patients with non-small cell lung cancer. The Patient: Patient-Centered Outcomes 
Research, 1(2), 105-113. 
Saligan, L. N., Luckenbaugh, D. A., Slonena, E. E., Machado-Vieira, R., & Zarate, C. A.(2016). 
An assessment of the anti-fatigue effects of ketamine from a double-blind, placebo-controlled, 
crossover study in bipolar disorder. Journal of affective disorders, 194, 115-119. 
Segal, B., Thomas, W., Rogers, T., Leon, J. M., Hughes, P., Patel, D., ... & Myers, S.(2008). 
Prevalence, severity, and predictors of fatigue in subjects with primary Sjögren's syndrome. 
Arthritis Care & Research, 59(12), 1780-1787. 
Sharpe, M., & Wilks, D. (2002). Fatigue. Bmj, 325(7362), 480-483. Sharp, R. (2015). The 
Hamilton rating scale for depression. Occupational Medicine, 65(4), 340-340. 
Tripp, N. H., Tarn, J., Natasari, A., Gillespie, C., Mitchell, S., Hackett, K. L., ... & Lanyon, P. 
(2016). Fatigue in primary Sjögren's syndrome is associated with lowerlevels of 
proinflammatory cytokines. RMD open, 2(2), e000282. 
Tyor, W. R., Glass, J. D., Griffin, J. W., Becker, P. S., McArthur, J. C., Bezman, L., &Griffin, D. 
E. (1992). Cytokine expression in the brain during the acquired immunodeficiency syndrome. 
Annals of Neurology: Official Journal of the AmericanNeurological Association and the Child 
Neurology Society, 31(4), 349-360. 

45 
Abbreviated Title: Ketamine on Fatigue 
Version Date: 04/18/2024 
Interventional Template v7 06.14.2023 
 
Van Leeuwen, M., Husson, O., Alberti, P., Arraras, J. I., Chinot, O. L., Costantini, A., ... & 
Holzner, B. (2018). Understanding the quality of life (QOL) issues in survivors ofcancer: 
towards the development of an EORTC QOL cancer survivorship questionnaire. Health and 
quality of life outcomes, 16(1), 114. 
Voss, J. G., Dodd, M., Portillo, C., & Holzemer, W. (2006). Theories of fatigue: application in 
HIV/AIDS. Journal of the Association of Nurses in AIDS Care, 17(1),37-50. 
Wang, X. S. (2008). Pathophysiology of cancer-related fatigue. Clinical journal ofoncology 
nursing, 12(5 Suppl), 11. 
Wessely, S., Nimnuan, C., & Sharpe, M. (1999). Functional somatic syndromes: one ormany? 
The Lancet, 354(9182), 936-939. 
White, P. F., Ham, J., Way, W. L., & Trevor, A. (1980). Pharmacology of ketamineisomers in 
surgical patients. Anesthesiology, 52(3), 231-239. 
White, P. D., Thomas, J. M., Amess, J., Crawford, D. H., Grover, S. A., Kangro, H. O., &Clare, 
A. W. (1998). Incidence, risk and prognosis of acute and chronic fatigue syndromes and 
psychiatric disorders after glandular fever. The British Journal of Psychiatry, 173(6), 475-481. 
Yellen, S. B., Cella, D. F., Webster, K., Blendowski, C., & Kaplan, E. (1997). Measuringfatigue 
and other anemia-related symptoms with the Functional Assessment of Cancer Therapy (FACT) 
measurement system. Journal of pain and symptom management, 13(2), 63-74. 
Zarate, C. A., Singh, J. B., Carlson, P. J., Brutsche, N. E., Ameli, R., Luckenbaugh, D.A., ... & 
Manji, H. K. (2006). A randomized trial of an N-methyl-D-aspartate antagonist in treatment- 
resistant major depression. Archives of general psychiatry, 63(8), 856-864. 
Zhou, F. L., Zhang, W. G., Wei, Y. C., Xu, K. L., Hui, L. Y., Wang, X. S., & Li, M. Z. (2005). 
Impact of comorbid anxiety and depression on quality of life and cellularimmunity changes in 
patients with digestive tract cancers. World Journal of Gastroenterology: WJG, 11(15), 2313. 
 
 

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24 
ATTACHMENTS/APPENDICES 
Appendices are uploaded to iRIS.  
Appendix 1a: Recruitment Flyer 
Appendix 1aa: Recruitment Flyer for Clinics 
Appendix 1b: Public Service Announcements 
Appendix 1c: Informational Cards for Clinicians and Investigators Appendix 1d: Informational 
Cards for Participants 
Appendix 2: Phone Pre-Screening Script Appendix 3: Eligibility Checklist Appendix 4: Schedule 
of Events 
Appendix 5: Post Infusion Discharge Instructions Appendix 7a: Randomization Survey 
(Participants) Appendix 7b: Randomization Survey (Study Team) Appendix 8: FDA Letter 
Appendix 9: Visit Schedule Appendix 10: Monthly Schedule 
Appendix 11: Recruitment Power Point Presentation Appendix 12: Educational Flyer 
Appendix C: NIH Information Sheet on Employee Research Participation