A Study on the Relationship Between Biodiversity of Intestinal Microbiota in Patients and Different Sedative-hypnotics Undergoing Mechanical Ventilation
Phase: NA
Conditions: Intestinal Microbiota, Mechanical Ventilation
Sponsor: Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University

Title：The Diversity of Intestinal Microbiota in Patients With 
Different Sedative-hypnotics and Mechanical Ventilation
NCT number:  03401736
date of document: 2018/01/01

In order to study the relationship between biodiversity of intestinal microbiota in 
patients and different sedative-hypnotics undergoing mechanical ventilation. As 
propofol and sevoflurane are widely used in clinical anesthesia as sedative 
anesthetics,so we choose these two drugs as our observation objects.
Twenty patients undergoing cochlear implantation with endotracheal intubation in 
department of anesthesiology, shanghai ninth people’s hospital, were allocated 
randomly to receive either propofol（n=10）or sevoflurane（n=10）. The parameter 
settings of ventilator (Drager,Germany Zeus Infinity Empowered)were set as follows: 
tidal volume 10 ml / kg, frequency 22 times / min. Oygen concentration was adjusted 
to maintain oxygen saturation no lower than 95%. The changes of hemodynamics 
were conventionally measured by the monitor. Propofol was used intravenously 
during the induction of general anesthesia (2.0mg / kg, 30 ~ 60s) in both groups. 
While in group propofol, continuous intravenous propofol maintenance (7 ~ 9mg · kg 
-1 · h-1) was used to achieve the stabilizing effect. And in group sevoflurane, 
inhalation (sevoflurane, 2.5-3.0 Vol%) maintenance was used. The BIS values of all 
the patients stay between 50 and 60. The average operation time was 5 hours.Faeces 
will be taken preoperative and postoperative so that the changes of the diversity of 
intestinal flora could be measured.
SPSS 21.0 software was used for data analysis. The measurement data from clinical 
studies are presented as means ± SEM (x ± s). The data between groups were subject 
to normal and variance homogeneity,independent sample t-test was used. Those who 
did not conform to normal distribution were tested by Wilcoxon rank sum test. The 
counting data were described by frequency and χ 2 test for groups comparison.