Effects of Aspirin on Emotional Reactivity, Memory, and Risk-taking
Phase: EARLY_PHASE1
Conditions: Healthy Adults
Sponsor: Ohio State University

Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
 
Methods 
Drug. Capsules containing 500mg Aspirin and identical capsules packed with 500mg 
microcrystalline cellulose prepared by Pharmacy Specialists (www.makerx.com), a licensed 
compounding pharmacy. 
Procedure. Participants will be randomly assigned to either placebo or 500 mg of Aspirin 
in a double-blind procedure. Participants will fill out background demographic and personality 
questionnaires for about 20-30 minutes after consuming drug. The tasks will begin after 60 
minutes has passed from drug or placebo intake, in order allow sufficient time for Aspirin to be 
absorbed into the brain (Cryer, 1998; Nagelschmitz, 2014). 
Emotional Image Rating Task: Each participant is presented with 120 images, drawn 
from the previously published stimuli used in Leal et al. (2014), Schaller (2011) and the 
International Affective Picture System (Lang, 1995), in a pseudo-random order according to the 
procedures in Durso et al. (2015). Images were pre-categorized on evaluative dimensions 
corresponding to extremely unpleasant (35 images), moderately unpleasant (15 images), neutral 
(20 images), moderately pleasant (15 images), and extremely pleasant (35 images). The 
participants will be asked to evaluate 60 of the images on their valence by being asked the 
question: “To what extent is this picture positive or negative?” using a 9-point scale with 4 being 
extremely positive, -4 being extremely negative, and 0 being neither positive nor negative. They 
will then be shown 60 different images, this time being asked: “To what extent does this picture 
make you feel an emotional reaction?” in order to rate their level of emotional arousal. A similar 
9-point scale was used, with 0 being “I feel little emotion” and 8 being “I feel an extreme amount 
of emotion.” These two blocks are in a consecutive, non-counterbalanced order.  

2 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
Balloon Analogue Risk Task: Once participants complete the emotional image rating 
task, they will complete the Balloon Analogue Risk Task (BART) to measure risk taking 
behavior (Lejuez, et al., 2002). In this task, participants have the opportunity to inflate 15 
balloons depicted on the computer screen. A trial begins with a small, uninflated balloon on the 
computer screen. Participants inflate the balloon, with each pump earning imaginary money, and 
were told their goal was to earn as much money as possible in the task. Participants could collect 
their total trial earnings and move them to a permanent bank at any point. However, participants 
also were told that the balloon can burst as early as the first trial and as late as when the balloon 
fills the entire computer screen. Bursts were accompanied by a bursting sound and popping 
animation. If the balloon burst prior to the participants choosing to collect their money, they lost 
any amount earned thus far on that trial, and had to move on to the next trial having added no 
money to their permanent bank. Participants were not told about the maximum number of pumps 
nor the likelihood of bursts, nor that, for each balloon, the first pump had a 1/128 probability of 
bursting, the second pump 1/127, and so on until on the 128thpump there wasa 1/1 probability of 
bursting. 
Memory Recognition Task: Participants are then asked to perform a memory recognition 
task based on the paradigm in Leal et al. (2014). Participants were shown 180 images and have 
to classify each as an image that they had seen in the emotional rating task or a new image. 
Images were divided equally among four different groups: 45 old images that appeared in the 
emotional rating task, 45 new high similarity lures that looked very closely like images shown in 
the emotional rating task (ex. an image of a crossing guard, after being shown an image of the 
same crossing guard from a slightly different angle in the first task), 45 new low similarity lures 
that looked somewhat like images shown in the emotional rating task (ex. an image of a dirty 

3 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
stall, after being shown an image of a dirty toilet in the first task), and 45 new foils that did not 
bare any resemblance to images from the emotional rating task (ex. an image of an airplane, after 
not being shown an image of aircraft in the first task). Each group was evenly composed of 9 
images from each extremely unpleasant, moderately unpleasant, neutral, moderately pleasant, 
and extremely pleasant image category. For analysis, d-prime (d’) scores were then calculated 
for each participant in each normative rating category. d’ is a measure of a participant’s signal 
detection ability and is unaffected by response bias. It was calculated by subtracting the z-score 
of each participant’s hit rate by the z-score of their false alarm rate for each normative rating 
category.     
Saliva Sample: Participants will then be asked to provide two saliva samples of 1.5 mL in 
order to measure prostaglandin levels in the saliva. Saliva samples are taken at the beginning of 
the experiment 5 minutes after the ingestion of drug or placebo, and at the end of the experiment 
after participants had completed all computerized tasks, approximately 90 minutes after ingestion 
of drug or placebo.   

4 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
Data Analysis Plan 
For analysis of the evaluations and arousal ratings of the emotional images, identical 
procedures to those done in Durso, Luttrell, & Way, (2015) will be performed. Specifically, 
responses will be subjected to a 2 (treatment: Aspirin, placebo) × 5 (normative rating: extremely 
unpleasant, moderately unpleasant, neutral, moderately pleasant, extremely pleasant) mixed-
model ANOVA. Participants who respond faster than 500msec will be dropped from analyses as 
they are deemed to not be taking the task seriously. 
For the Balloon Analogue Risk Task, the primary dependent measure will be adjusted 
average number of pumps (the number of pumps on trials where the balloon didn’t burst) 
following the initial work in this area (Lejuez, et al., 2002). 
For the memory recognition task, the d’ score will be subjected to the same analytical 
approach as that for the evaluations and arousal ratings.  
 
 

5 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
References 
Cryer B, Feldman M (1998). Cyclooxygenase-1 and cyclooxygenase-2 selectivity of widely used 
nonsteroidal anti-inflammatory drugs. American Journal of Medicine: 104(5), 413–421. 
doi: 10.1016/s0002-9343(98)00091-6. 
Durso GRO, Luttrell A, Way BM (2015). Over-the-counter relief from pains and pleasures alike: 
Acetaminophen blunts evaluation sensitivity to both negative and positive stimuli. 
Psychological Science: 26(6), 750–758. 
Hayes, A. F. (2012). PROCESS: A versatile computational tool for observed variable mediation, 
moderation, and conditional process modeling [White paper]. Retrieved from 
http://www.afhayes.com/public/process2012. 
Lang P.J. (1995). The Emotion Probe: Studies of Motivation and Attention. American 
Psychologist: 50(5): 372-385. 
Leal S.L.,Tighe S.K.,Yassa M.A. (2014). Asymmetric effects of emotion on mnemonic 
interference. Neurobiol Learn Mem,111:41-48. 
Lejuez, C. W., Read J.P., Kahler C.W., Richards J.B., Ramsey S.E., Stuart G.L., Strong D.R., 
Brown R.A. (2002). Evaluation of a Behavioral Measure of Risk Taking: The Balloon 
Analogue Risk Task (BART). Journal of Experimental Psychology: Applied, 8(2):75–84. 
Nagelschmitz J, Blunck M, Kraetzschmar J, et al (2014). Pharmacokinetics and 
pharmacodynamics of acetylsalicylic acid after intravenous and oral administration to 
healthy volunteers. Clinical Pharmacology: 6(1), 51–9. 

6 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
Schaller M., Miller G.E., Gervais W.M., Yager S., Chen E. (2009). Mere Visual Perception of 
Other People’s Disease Symptoms Facilitates a More Aggressive Immune Response. 
Psychological Science: 21(5), 649–652. doi: 10.1177/0956797610368064 
 
 

7 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
Appendix 
 
Images used in Emotional Image Rating Task 
 
All images from both the emotional image rating task and the memory recognition task 
were drawn from the previously published stimuli used in Leal et al. (2014), Schaller (2011) and 
the International Affective Picture System (Lang, 1995). The table below lists the set, image 
number, and valance category for each image used.  
Image Set 
Image Number 
Valance Category 
International Affective Picture System 
1052 
Extremely Unpleasant 
International Affective Picture System 
1111 
Extremely Unpleasant 
International Affective Picture System 
1201 
Extremely Unpleasant 
Leal et al. (2014) 
10040a 
Extremely Unpleasant 
Leal et al. (2014) 
10041a 
Extremely Unpleasant 
Leal et al. (2014) 
10044a 
Extremely Unpleasant 
Schaller (2011) 
d6 
Extremely Unpleasant 
Schaller (2011) 
d2 
Extremely Unpleasant 
Schaller (2011) 
d4 
Extremely Unpleasant 
Schaller (2011) 
d5 
Extremely Unpleasant 
Leal et al. (2014) 
10012a 
Extremely Unpleasant 
Leal et al. (2014) 
10023a 
Extremely Unpleasant 
Leal et al. (2014) 
10032a 
Extremely Unpleasant 
Leal et al. (2014) 
10048a 
Extremely Unpleasant 
International Affective Picture System 
6560 
Extremely Unpleasant 
Leal et al. (2014) 
10035a 
Extremely Unpleasant 
Leal et al. (2014) 
10002a 
Extremely Unpleasant 
Leal et al. (2014) 
10009a 
Extremely Unpleasant 
Leal et al. (2014) 
10025a 
Extremely Unpleasant 
Leal et al. (2014) 
10031a 
Extremely Unpleasant 
Leal et al. (2014) 
10033a 
Extremely Unpleasant 
International Affective Picture System 
3010 
Extremely Unpleasant 
International Affective Picture System 
3061 
Extremely Unpleasant 
International Affective Picture System 
3130 
Extremely Unpleasant 
International Affective Picture System 
3140 
Extremely Unpleasant 
International Affective Picture System 
3150 
Extremely Unpleasant 
Leal et al. (2014) 
10038a 
Extremely Unpleasant 
Leal et al. (2014) 
10039a 
Extremely Unpleasant 
Leal et al. (2014) 
10001a 
Extremely Unpleasant 
Leal et al. (2014) 
10010a 
Extremely Unpleasant 
Leal et al. (2014) 
10015a 
Extremely Unpleasant 
Leal et al. (2014) 
10019a 
Extremely Unpleasant 
Leal et al. (2014) 
10020a 
Extremely Unpleasant 
Leal et al. (2014) 
10029a 
Extremely Unpleasant 
Leal et al. (2014) 
10005a 
Extremely Unpleasant 
Schaller (2011) 
d10 
Moderately Unpleasant 
Schaller (2011) 
d3 
Moderately Unpleasant 
International Affective Picture System 
3250 
Moderately Unpleasant 
International Affective Picture System 
9594 
Moderately Unpleasant 

8 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
Leal et al. (2014) 
10013a 
Moderately Unpleasant 
Leal et al. (2014) 
10043a 
Moderately Unpleasant 
International Affective Picture System 
2457 
Moderately Unpleasant 
Leal et al. (2014) 
10022a 
Moderately Unpleasant 
Leal et al. (2014) 
10028a 
Moderately Unpleasant 
Leal et al. (2014) 
10006a 
Moderately Unpleasant 
Leal et al. (2014) 
10016a 
Moderately Unpleasant 
Leal et al. (2014) 
10021a 
Moderately Unpleasant 
Leal et al. (2014) 
10036a 
Moderately Unpleasant 
Leal et al. (2014) 
10045a 
Moderately Unpleasant 
Leal et al. (2014) 
10046a 
Moderately Unpleasant 
Leal et al. (2014) 
20003a 
Neutral 
Leal et al. (2014) 
20010a 
Neutral 
Leal et al. (2014) 
20012a 
Neutral 
Leal et al. (2014) 
20015a 
Neutral 
Leal et al. (2014) 
20016a 
Neutral 
Leal et al. (2014) 
20017a 
Neutral 
Leal et al. (2014) 
20020a 
Neutral 
Leal et al. (2014) 
20022a 
Neutral 
Leal et al. (2014) 
20023a 
Neutral 
Leal et al. (2014) 
20025a 
Neutral 
Leal et al. (2014) 
20026a 
Neutral 
Leal et al. (2014) 
20028a 
Neutral 
Leal et al. (2014) 
20029a 
Neutral 
Leal et al. (2014) 
20031a 
Neutral 
Leal et al. (2014) 
20035a 
Neutral 
Leal et al. (2014) 
20037a 
Neutral 
Leal et al. (2014) 
20038a 
Neutral 
Leal et al. (2014) 
20041a 
Neutral 
Leal et al. (2014) 
20043a 
Neutral 
Leal et al. (2014) 
20048a 
Neutral 
Leal et al. (2014) 
20006a 
Moderately Pleasant 
Leal et al. (2014) 
20007a 
Moderately Pleasant 
Leal et al. (2014) 
20014a 
Moderately Pleasant 
Leal et al. (2014) 
20019a 
Moderately Pleasant 
Leal et al. (2014) 
30009a 
Moderately Pleasant 
Leal et al. (2014) 
30010a 
Moderately Pleasant 
Leal et al. (2014) 
30025a 
Moderately Pleasant 
Leal et al. (2014) 
30030a 
Moderately Pleasant 
Leal et al. (2014) 
30031a 
Moderately Pleasant 
Leal et al. (2014) 
30033a 
Moderately Pleasant 
Leal et al. (2014) 
30034a 
Moderately Pleasant 
Leal et al. (2014) 
30035a 
Moderately Pleasant 
International Affective Picture System 
4800 
Moderately Pleasant 
Leal et al. (2014) 
30039a 
Moderately Pleasant 
International Affective Picture System 
4647 
Moderately Pleasant 
International Affective Picture System 
4220 
Extremely Pleasant 
International Affective Picture System 
4290 
Extremely Pleasant 
International Affective Picture System 
4660 
Extremely Pleasant 
International Affective Picture System 
4670 
Extremely Pleasant 
International Affective Picture System 
4680 
Extremely Pleasant 
Leal et al. (2014) 
30002a 
Extremely Pleasant 
Leal et al. (2014) 
30003a 
Extremely Pleasant 

9 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
Leal et al. (2014) 
30005a 
Extremely Pleasant 
Leal et al. (2014) 
30006a 
Extremely Pleasant 
Leal et al. (2014) 
30008a 
Extremely Pleasant 
Leal et al. (2014) 
30011a 
Extremely Pleasant 
Leal et al. (2014) 
30012a 
Extremely Pleasant 
Leal et al. (2014) 
30013a 
Extremely Pleasant 
Leal et al. (2014) 
30015a 
Extremely Pleasant 
Leal et al. (2014) 
30016a 
Extremely Pleasant 
Leal et al. (2014) 
30018a 
Extremely Pleasant 
Leal et al. (2014) 
30019a 
Extremely Pleasant 
Leal et al. (2014) 
30020a 
Extremely Pleasant 
Leal et al. (2014) 
30022a 
Extremely Pleasant 
Leal et al. (2014) 
30023a 
Extremely Pleasant 
Leal et al. (2014) 
30026a 
Extremely Pleasant 
Leal et al. (2014) 
30027a 
Extremely Pleasant 
Leal et al. (2014) 
30028a 
Extremely Pleasant 
Leal et al. (2014) 
30029a 
Extremely Pleasant 
Leal et al. (2014) 
30036a 
Extremely Pleasant 
Leal et al. (2014) 
30040a 
Extremely Pleasant 
Leal et al. (2014) 
30041a 
Extremely Pleasant 
Leal et al. (2014) 
30042a 
Extremely Pleasant 
Leal et al. (2014) 
30045a 
Extremely Pleasant 
Leal et al. (2014) 
30046a 
Extremely Pleasant 
Leal et al. (2014) 
30047a 
Extremely Pleasant 
Leal et al. (2014) 
30049a 
Extremely Pleasant 
Leal et al. (2014) 
30051a 
Extremely Pleasant 
Leal et al. (2014) 
30050a 
Extremely Pleasant 
 
 
 

10 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
Images used in Memory Recognition Task 
 
Image Set 
Image Number 
Valance Category 
Target/Foil/Lure 
Leal et. al (2014) 
10012a 
Extremely Unpleasant 
Target 
Leal et. al (2014) 
10039a 
Extremely Unpleasant 
Target 
Leal et. al (2014) 
10041a 
Extremely Unpleasant 
Target 
Leal et. al (2014) 
10009a 
Extremely Unpleasant 
Target 
Leal et. al (2014) 
10015a 
Extremely Unpleasant 
Target 
Schaller (2012) 
d6 
Extremely Unpleasant 
Target 
Schaller (2012) 
d5 
Extremely Unpleasant 
Target 
International Affective Picture System 
6560 
Extremely Unpleasant 
Target 
Leal et. al (2014) 
10005a 
Extremely Unpleasant 
Target 
Leal et. al (2014) 
10043a 
Moderately Unpleasant 
Target 
Leal et. al (2014) 
10046a 
Moderately Unpleasant 
Target 
Leal et. al (2014) 
10022a 
Moderately Unpleasant 
Target 
Leal et. al (2014) 
10013a 
Moderately Unpleasant 
Target 
Leal et. al (2014) 
10016a 
Moderately Unpleasant 
Target 
Leal et. al (2014) 
10036a 
Moderately Unpleasant 
Target 
Schaller (2012) 
d3 
Moderately Unpleasant 
Target 
Schaller (2012) 
d10 
Moderately Unpleasant 
Target 
International Affective Picture System 
2457 
Moderately Unpleasant 
Target 
Leal et. al (2014) 
20029a 
Neutral 
Target 
Leal et. al (2014) 
20026a 
Neutral 
Target 
Leal et. al (2014) 
20025a 
Neutral 
Target 
Leal et. al (2014) 
20028a 
Neutral 
Target 
Leal et. al (2014) 
20022a 
Neutral 
Target 
Leal et. al (2014) 
20003a 
Neutral 
Target 
Leal et. al (2014) 
20010a 
Neutral 
Target 
Leal et. al (2014) 
20012a 
Neutral 
Target 
Leal et. al (2014) 
20017a 
Neutral 
Target 
International Affective Picture System 
4647 
Moderately Pleasant 
Target 
Leal et. al (2014) 
30034a 
Moderately Pleasant 
Target 
International Affective Picture System 
4800 
Moderately Pleasant 
Target 
Leal et. al (2014) 
30035a 
Moderately Pleasant 
Target 
Leal et. al (2014) 
30010a 
Moderately Pleasant 
Target 
Leal et. al (2014) 
20007a 
Moderately Pleasant 
Target 
Leal et. al (2014) 
20006a 
Moderately Pleasant 
Target 
Leal et. al (2014) 
30009a 
Moderately Pleasant 
Target 
Leal et. al (2014) 
30030a 
Moderately Pleasant 
Target 
International Affective Picture System 
4220 
Extremely Pleasant 
Target 
Leal et. al (2014) 
30003a 
Extremely Pleasant 
Target 
International Affective Picture System 
4660 
Extremely Pleasant 
Target 
Leal et. al (2014) 
30051a 
Extremely Pleasant 
Target 
International Affective Picture System 
4290 
Extremely Pleasant 
Target 
Leal et. al (2014) 
30012a 
Extremely Pleasant 
Target 
International Affective Picture System 
4670 
Extremely Pleasant 
Target 
Leal et. al (2014) 
30018a 
Extremely Pleasant 
Target 
Leal et. al (2014) 
30019a 
Extremely Pleasant 
Target 
Leal et. al (2014) 
10023c 
Extremely Unpleasant 
High Similarity Lure 
Leal et. al (2014) 
10005c 
Extremely Unpleasant 
High Similarity Lure 
Leal et. al (2014) 
10035c 
Extremely Unpleasant 
High Similarity Lure 
Leal et. al (2014) 
10002c 
Extremely Unpleasant 
High Similarity Lure 

11 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
Leal et. al (2014) 
10031c 
Extremely Unpleasant 
High Similarity Lure 
Leal et. al (2014) 
10001c 
Extremely Unpleasant 
High Similarity Lure 
Leal et. al (2014) 
10038c 
Extremely Unpleasant 
High Similarity Lure 
Leal et. al (2014) 
10029c 
Extremely Unpleasant 
High Similarity Lure 
Leal et. al (2014) 
10025c 
Extremely Unpleasant 
High Similarity Lure 
Leal et. al (2014) 
10043c 
Moderately Unpleasant 
High Similarity Lure 
Leal et. al (2014) 
10013c 
Moderately Unpleasant 
High Similarity Lure 
Leal et. al (2014) 
10046c 
Moderately Unpleasant 
High Similarity Lure 
Leal et. al (2014) 
10045c 
Moderately Unpleasant 
High Similarity Lure 
Leal et. al (2014) 
10021c 
Moderately Unpleasant 
High Similarity Lure 
Leal et. al (2014) 
10022c 
Moderately Unpleasant 
High Similarity Lure 
Leal et. al (2014) 
10046c 
Moderately Unpleasant 
High Similarity Lure 
Leal et. al (2014) 
10036c 
Moderately Unpleasant 
High Similarity Lure 
Leal et. al (2014) 
10006c 
Moderately Unpleasant 
High Similarity Lure 
Leal et. al (2014) 
20037c 
Neutral 
High Similarity Lure 
Leal et. al (2014) 
20029c 
Neutral 
High Similarity Lure 
Leal et. al (2014) 
20025c 
Neutral 
High Similarity Lure 
Leal et. al (2014) 
20043c 
Neutral 
High Similarity Lure 
Leal et. al (2014) 
20031c 
Neutral 
High Similarity Lure 
Leal et. al (2014) 
20035c 
Neutral 
High Similarity Lure 
Leal et. al (2014) 
20003c 
Neutral 
High Similarity Lure 
Leal et. al (2014) 
20010c 
Neutral 
High Similarity Lure 
Leal et. al (2014) 
20015c 
Neutral 
High Similarity Lure 
Leal et. al (2014) 
20019c 
Moderately Pleasant 
High Similarity Lure 
Leal et. al (2014) 
30034c 
Moderately Pleasant 
High Similarity Lure 
Leal et. al (2014) 
20014c 
Moderately Pleasant 
High Similarity Lure 
Leal et. al (2014) 
30033c 
Moderately Pleasant 
High Similarity Lure 
Leal et. al (2014) 
30039c 
Moderately Pleasant 
High Similarity Lure 
Leal et. al (2014) 
30035c 
Moderately Pleasant 
High Similarity Lure 
Leal et. al (2014) 
20006c 
Moderately Pleasant 
High Similarity Lure 
Leal et. al (2014) 
30009c 
Moderately Pleasant 
High Similarity Lure 
Leal et. al (2014) 
30010c 
Moderately Pleasant 
High Similarity Lure 
Leal et. al (2014) 
30019c 
Extremely Pleasant 
High Similarity Lure 
Leal et. al (2014) 
30013c 
Extremely Pleasant 
High Similarity Lure 
Leal et. al (2014) 
30016c 
Extremely Pleasant 
High Similarity Lure 
Leal et. al (2014) 
30023c 
Extremely Pleasant 
High Similarity Lure 
Leal et. al (2014) 
30046c 
Extremely Pleasant 
High Similarity Lure 
Leal et. al (2014) 
30008c 
Extremely Pleasant 
High Similarity Lure 
Leal et. al (2014) 
30002c 
Extremely Pleasant 
High Similarity Lure 
Leal et. al (2014) 
30005c 
Extremely Pleasant 
High Similarity Lure 
Leal et. al (2014) 
30020c 
Extremely Pleasant 
High Similarity Lure 
Leal et. al (2014) 
10032b 
Extremely Unpleasant 
Low Similarity Lure 
Leal et. al (2014) 
10039b 
Extremely Unpleasant 
Low Similarity Lure 
Leal et. al (2014) 
10048b 
Extremely Unpleasant 
Low Similarity Lure 
Leal et. al (2014) 
10031b 
Extremely Unpleasant 
Low Similarity Lure 
Leal et. al (2014) 
10040b 
Extremely Unpleasant 
Low Similarity Lure 
Leal et. al (2014) 
10020b 
Extremely Unpleasant 
Low Similarity Lure 
Leal et. al (2014) 
10012b 
Extremely Unpleasant 
Low Similarity Lure 
Leal et. al (2014) 
10023b 
Extremely Unpleasant 
Low Similarity Lure 
Leal et. al (2014) 
10035b 
Extremely Unpleasant 
Low Similarity Lure 
Leal et. al (2014) 
10013b 
Moderately Unpleasant 
Low Similarity Lure 
Leal et. al (2014) 
10036b 
Moderately Unpleasant 
Low Similarity Lure 
Leal et. al (2014) 
10006b 
Moderately Unpleasant 
Low Similarity Lure 

12 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
Leal et. al (2014) 
10016b 
Moderately Unpleasant 
Low Similarity Lure 
Leal et. al (2014) 
10021b 
Moderately Unpleasant 
Low Similarity Lure 
Leal et. al (2014) 
10028b 
Moderately Unpleasant 
Low Similarity Lure 
Leal et. al (2014) 
10043b 
Moderately Unpleasant 
Low Similarity Lure 
Leal et. al (2014) 
10022b 
Moderately Unpleasant 
Low Similarity Lure 
Leal et. al (2014) 
10046b 
Moderately Unpleasant 
Low Similarity Lure 
Leal et. al (2014) 
20048b 
Neutral 
Low Similarity Lure 
Leal et. al (2014) 
20026b 
Neutral 
Low Similarity Lure 
Leal et. al (2014) 
20025b 
Neutral 
Low Similarity Lure 
Leal et. al (2014) 
20022b 
Neutral 
Low Similarity Lure 
Leal et. al (2014) 
20017b 
Neutral 
Low Similarity Lure 
Leal et. al (2014) 
20016b 
Neutral 
Low Similarity Lure 
Leal et. al (2014) 
20003b 
Neutral 
Low Similarity Lure 
Leal et. al (2014) 
20010b 
Neutral 
Low Similarity Lure 
Leal et. al (2014) 
20012b 
Neutral 
Low Similarity Lure 
Leal et. al (2014) 
30010b 
Moderately Pleasant 
Low Similarity Lure 
Leal et. al (2014) 
20006b 
Moderately Pleasant 
Low Similarity Lure 
Leal et. al (2014) 
30030b 
Moderately Pleasant 
Low Similarity Lure 
Leal et. al (2014) 
20007b 
Moderately Pleasant 
Low Similarity Lure 
Leal et. al (2014) 
20014b 
Moderately Pleasant 
Low Similarity Lure 
Leal et. al (2014) 
30031b 
Moderately Pleasant 
Low Similarity Lure 
Leal et. al (2014) 
30009b 
Moderately Pleasant 
Low Similarity Lure 
Leal et. al (2014) 
30033b 
Moderately Pleasant 
Low Similarity Lure 
Leal et. al (2014) 
30035b 
Moderately Pleasant 
Low Similarity Lure 
Leal et. al (2014) 
30003b 
Extremely Pleasant 
Low Similarity Lure 
Leal et. al (2014) 
30011b 
Extremely Pleasant 
Low Similarity Lure 
Leal et. al (2014) 
30040b 
Extremely Pleasant 
Low Similarity Lure 
Leal et. al (2014) 
30049b 
Extremely Pleasant 
Low Similarity Lure 
Leal et. al (2014) 
30018b 
Extremely Pleasant 
Low Similarity Lure 
Leal et. al (2014) 
30029b 
Extremely Pleasant 
Low Similarity Lure 
Leal et. al (2014) 
30002b 
Extremely Pleasant 
Low Similarity Lure 
Leal et. al (2014) 
30036b 
Extremely Pleasant 
Low Similarity Lure 
Leal et. al (2014) 
30026b 
Extremely Pleasant 
Low Similarity Lure 
Leal et. al (2014) 
40041a 
Extremely Unpleasant 
Foil 
Leal et. al (2014) 
40038a 
Extremely Unpleasant 
Foil 
Leal et. al (2014) 
40006a 
Extremely Unpleasant 
Foil 
Leal et. al (2014) 
40009a 
Extremely Unpleasant 
Foil 
Leal et. al (2014) 
40022a 
Extremely Unpleasant 
Foil 
Leal et. al (2014) 
40035a 
Extremely Unpleasant 
Foil 
Leal et. al (2014) 
40002a 
Extremely Unpleasant 
Foil 
Leal et. al (2014) 
40010a 
Extremely Unpleasant 
Foil 
Leal et. al (2014) 
40011a 
Extremely Unpleasant 
Foil 
Leal et. al (2014) 
40007a 
Moderately Unpleasant 
Foil 
Leal et. al (2014) 
40013a 
Moderately Unpleasant 
Foil 
Leal et. al (2014) 
40028a 
Moderately Unpleasant 
Foil 
Leal et. al (2014) 
40031a 
Moderately Unpleasant 
Foil 
Leal et. al (2014) 
40044a 
Moderately Unpleasant 
Foil 
Leal et. al (2014) 
40029a 
Moderately Unpleasant 
Foil 
Leal et. al (2014) 
40008a 
Moderately Unpleasant 
Foil 
Leal et. al (2014) 
40012a 
Moderately Unpleasant 
Foil 
Leal et. al (2014) 
40015a 
Moderately Unpleasant 
Foil 
Leal et. al (2014) 
50005a 
Neutral 
Foil 
Leal et. al (2014) 
50007a 
Neutral 
Foil 

13 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
Leal et. al (2014) 
50010a 
Neutral 
Foil 
Leal et. al (2014) 
50014a 
Neutral 
Foil 
Leal et. al (2014) 
50016a 
Neutral 
Foil 
Leal et. al (2014) 
50042a 
Neutral 
Foil 
Leal et. al (2014) 
50013a 
Neutral 
Foil 
Leal et. al (2014) 
50017a 
Neutral 
Foil 
Leal et. al (2014) 
50018a 
Neutral 
Foil 
Leal et. al (2014) 
60001a 
Moderately Pleasant 
Foil 
Leal et. al (2014) 
60003a 
Moderately Pleasant 
Foil 
Leal et. al (2014) 
60011a 
Moderately Pleasant 
Foil 
Leal et. al (2014) 
60019a 
Moderately Pleasant 
Foil 
Leal et. al (2014) 
60002a 
Moderately Pleasant 
Foil 
Leal et. al (2014) 
60004a 
Moderately Pleasant 
Foil 
Leal et. al (2014) 
60017a 
Moderately Pleasant 
Foil 
Leal et. al (2014) 
60032a 
Moderately Pleasant 
Foil 
Leal et. al (2014) 
60041a 
Moderately Pleasant 
Foil 
Leal et. al (2014) 
60048a 
Extremely Pleasant 
Foil 
Leal et. al (2014) 
60043a 
Extremely Pleasant 
Foil 
Leal et. al (2014) 
60038a 
Extremely Pleasant 
Foil 
Leal et. al (2014) 
60033a 
Extremely Pleasant 
Foil 
Leal et. al (2014) 
60027a 
Extremely Pleasant 
Foil 
Leal et. al (2014) 
60010a 
Extremely Pleasant 
Foil 
Leal et. al (2014) 
60039a 
Extremely Pleasant 
Foil 
Leal et. al (2014) 
60024a 
Extremely Pleasant 
Foil 
Leal et. al (2014) 
60020a 
Extremely Pleasant 
Foil 
 
 
 

14 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
Questionnaires 
Background Measures 
Recent Health Behaviors Questionnaire 
 
 
1. 
 
What is your height?    ______feet _____inches 
 
 
2. 
 
What is your weight?  ____________ (in pounds) 
 
 
3. 
 
What time did you go to bed last night?  ____________ 
 
 
4. 
 
How long (in minutes) did it take you to fall asleep last night?  ____________ 
 
 
5. 
 
What time did you get up this morning? _____________ 
 
 
6. 
 
On a scale of 1 to 10, how well did you sleep? (1=poorly, 10=well)  ____________ 
 
 
7. 
 
How many hours of actual sleep did you get? (This may be different than the number of 
hours you spent in bed?  ____________________ 
 
 
8. 
 
Approximately how long ago did you last eat?  ____________ 
 
Was it a (indicate one): 
    Snack                Light Meal              Full Meal 
 
 
9. 
 
Have you done any aerobic exercise today?                                   Yes           No 
 
If yes, how many minutes? _____________ 
 
If yes, how long ago (in hours)? _____________ 
 
10. 
 
 On an average week, how many days do you do aerobic exercise? __________ 
 

15 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
 
11. 
 
When you do exercise, how long do you usually work out?  ____________ (in minutes) 
 
 
 
1. 
 
In general, would you say that your health is (indicate one): 
 
Excellent            Very Good                Good                   Fair                  Poor 
 
Please answer the 
following 3 questions by 
circling a number on the 
right: 
Strongly 
Disagree 
 
 
 
Strongly 
Agree 
 
2. 
Over the last couple of 
days, I have not been 
feeling well. 
1 
2 
3 
4 
5 
 
3. 
Lately, I have been 
feeling a little under 
the weather 
1 
2 
3 
4 
5 
 
4. 
 
I have felt sick within 
the last week. 
 
1 
2 
3 
4 
5 
 
5. 
When was the last time you had a cold, flu, dental infection, or other infection? 
____Today 
 
____A couple of days ago 
 
____A week ago 
____A couple of weeks ago 
____A month ago 
____A few months ago 
____A year or more ago 
 
6.  
 
Did you seek medical care for any sort of cold, flu, or infection in the last 3 months?      Yes        
No 
 
If yes, how many times in the last 3 months did you go to the health center for an 
illness?__________________ 
7.  
 
Did you take any over-the-counter or prescription medications for a cold, flu, or          Yes        
No 
any infection in the last 3 months? 
 

16 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
If yes, what medications_______________________________________________ 
 
 
Please answer the following questions by circling a response on the right: 
 
 
1.  In the box below, please list all medications AND vitamins or supplements you are currently 
taking, including pills, patches, lotions or injections. Medications can be prescription medications 
or over-the-counter medications. Be sure to include the NAME, DOSE, AMOUNT and FREQUENCY 
you take the medications or supplements, putting a comma between each one. If the medication 
or supplement does not have a particular dose, please put "NA" after name and before frequency. 
See examples below. 
 
Example 1: Oxytrol 3.9 mg 1 patch every 4 days, Femgest progesterone cream no dosage apply to 
hands once per day, Synthroid 75 mcg 1 pill once per day. 
 
Example 2: Timoptic 0.25% 2 drops per eye twice per day, Saw Palmetto 160 mg 1 pill twice per day, 
Zocor 20 mg each night. 
 
Example 3: Calcium 333mg/pill 3 pills per day, Vitamin C 500mg 1 pill per day, Vitamin D 1000mg 1 
pill per day. 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
2. 
 
Are you currently taking birth control medication?      Yes           No 
 
 
3. 
 
How often do you typically use marijuana? (indicate one) 
 
___ Almost every day                                  ___ At least once a month 
___ Several times a week                             ___ Several times a year 
___ At least once a week                              ___ At least once a year 

17 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
___ Several times a month                           ___ Never 
 
 
 
 
4. 
How long ago did you last use marijuana?  (If in the last day, please indicate the number of 
hours. If not record the number of days, months, or years.) 
 
Hours:__________  Days: __________  Months: __________  Years: __________ 
 
_____I have never used marijuana. 
 
5. 
 
How many cigarettes per day do you smoke (indicate one response)? 
 
   0          
1 to 10     
10 to 20 
20 to 30 
30 or more 
 
 
6. 
 
If you smoke, how long has it been since your last cigarette? (Please indicate the number of 
minutes and hours.) 
 
                       Minutes:__________  Hours: __________   
 
7. 
 
When was the last time you consumed an alcoholic beverage?   (If in the last day, please 
indicate the number of hours. If not record the number of days, months, or years.) 
 
Hours:__________  Days: __________  Months: __________  Years: __________ 
 
_____I do not drink alcohol. 
 
 
Please answer the following 3 questions by circling a response on the right: 
 
8. 
How 
often do 
you have 
a drink 
containing 
alcohol? 
 
Never 
 
Monthly 
2 - 4 times a 
month  
2 – 3 
times a 
week 
4 or more 
times a week 
 
9. 
How 
many 
drinks 
containing 
alcohol do 
you have 
on a 
typical 
1 or 2 
3 or 4 
5 or 6 
7 to 9 
10 or 
more 

18 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
day when 
you are 
drinking? 
 
 
 
 
 
 
 
 
10. 
How 
often do 
you have 
six or 
more 
drinks on 
one 
occasion?  
Never 
Less 
than 
Monthly 
Monthly 
Weekly 
Daily or 
almost 
Daily 
 
 
 
11. 
 
Do you have arthritis, rheumatoid arthritis, or joint problems?               Yes           No 
 
 
12. 
 
Do you have arthritis or any immune disorders that might lead to  
immunodeficiency such as HIV?                                                              Yes           No 
 
 
13. 
 
Do you have any chronic illnesses that affect the  
endocrine system (e.g Cushing’s Disease)?                                           Yes           No 
 
 
14. 
Are you diabetic (Type I or II)?                                                                Yes           No 
 
 
 
 
 

19 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
Subjective Socioeconomic Status Measure 
 
 
 
 
 
 

20 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
Political Ideology (Wilson, 1968) 
G.D. Wilson, J.R. Patterson, A new measure of conservatism, Br. J. Soc. Clin.Psychol. 7 (1968) 264–269. 
Mofied by Mills, M., Gonzalez, F. J., Giuseffi, K., Sievert, B., Smith, K. B., Hibbing, J. R., & 
Dodd, M. D. (2016). Political conservatism predicts asymmetries in emotional scene 
memory. Behavioural brain research, 306, 84–90. 
 
Indicate your agreement with the following issues: 
 
strongly 
disagree with 
(1) 
disagree with 
(2) 
uncertain 
about (3) 
agree with (4) 
strongly agree 
with (5) 
school prayer 
(1)  
o  
o  
o  
o  
o  
pacifism (2)  
o  
o  
o  
o  
o  
stopping 
immigration 
(3)  
o  
o  
o  
o  
o  
death penalty 
(4)  
o  
o  
o  
o  
o  
government-
arranged 
healthcare (5)  o  
o  
o  
o  
o  
premarital sex 
(6)  
o  
o  
o  
o  
o  
gay marriage 
(7)  
o  
o  
o  
o  
o  
abortion rights 
(8)  
o  
o  
o  
o  
o  
evolution (9)  o  
o  
o  
o  
o  
biblical truth 
(10)  
o  
o  
o  
o  
o  
increased 
welfare 
spending (11)  o  
o  
o  
o  
o  
protecting gun 
rights (12)  
o  
o  
o  
o  
o  
increasing 
military 
spending (13)  o  
o  
o  
o  
o  
government 
regulation of 
business (14)  o  
o  
o  
o  
o  
small 
government 
(15)  
o  
o  
o  
o  
o  

21 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
foreign aide 
(16)  
o  
o  
o  
o  
o  
lowering taxes 
(17)  
o  
o  
o  
o  
o  
stem cell 
research (18)  o  
o  
o  
o  
o  
abstinence-
only sex 
education (19)  o  
o  
o  
o  
o  
allowing 
torture of 
terrorism 
suspects (20)  
o  
o  
o  
o  
o  
 
When it comes to politics in general, do you usually think of yourself as liberal, moderate, or 
conservative? 
o very liberal  (1)  
o liberal  (2)  
o somewhat liberal  (3)  
o moderate  (4)  
o somewhat conservative  (5)  
o conservative  (6)  
o very conservative  (7)  
 
 
In general, I consider myself a: 
o Democrat  (1)  
o Republican  (2)  
o Libertarian  (3)  
o Other  (4)  
 
 
When it comes to politics in general, do you usually think of yourself as liberal, moderate, conservative 
or something else? 
 
Very 
Liberal 
 
   Liberal 
Somewhat 
Liberal 
 
Moderate 
Somewhat 
Conservative 
 
Conservative 
Very 
Conservative 
1 
2 
3 
4 
5 
6 
7 

22 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
 
On social issues, you consider yourself to be: 
 
Very 
Liberal 
 
   Liberal 
Somewhat 
Liberal 
 
Moderate 
Somewhat 
Conservative 
 
Conservative 
Very 
Conservative 
1 
2 
3 
4 
5 
6 
7 
 
On foreign policy issues, you consider yourself to be: 
 
Very 
Liberal 
 
   Liberal 
Somewhat 
Liberal 
 
Moderate 
Somewhat 
Conservative 
 
Conservative 
Very 
Conservative 
1 
2 
3 
4 
5 
6 
7 
 
On economic issues, you consider yourself to be: 
 
Very 
Liberal 
 
   Liberal 
Somewhat 
Liberal 
 
Moderate 
Somewhat 
Conservative 
 
Conservative 
Very 
Conservative 
1 
2 
3 
4 
5 
6 
7 
 
 
 
 

23 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
Emotionality Measures  
Domain Specific Risk Taking (Blais & Weber, 2006) 
Blais, A. & Weber, E. U. (2006). A domain-specific risk-taking (DOSPERT) scale for adult populations. 
Judgment and Decision Making, 1(1): 33-47. 
 
Risk Perception 
People often see some risk in situations that contain uncertainty about what the outcome or 
consequences will be and for which there is the possibility of negative consequences.  However, 
riskiness is a very personal and intuitive notion, and we are interested in your gut level assessment of 
how risky each situation or behavior is. For each of the following statements, please indicate how risky 
you perceive each situation. Provide a rating from Not at all Risky to Extremely Risky, using the following 
scale: 
1 Not at all Risky  2 Slightly Risky  3 Somewhat Risky  4 Moderately Risky  5 Risky  6 Very Risky  7Extremely Risky 
Expected Benefits 
For each of the following statements, please indicate the benefits you would obtain from each  
situation. Provide a rating from 1 to 7, using the following scale: 
1 No Benefits at all       2           3          4 Moderate Benefits        5          6        7 Great Benefits 
Items 
 
1.Admitting that your tastes are different from those of a friend. (S) 
2.Going camping in the wilderness. (R) 
3.Betting a day’s income at the horse races. (F/G) 
4.Investing 10% of your annual income in a moderate growth diversified fund. (F/I) 
5.Drinking heavily at a social function. (H/S) 
6.Taking some questionable deductions on your income tax return. (E)  
7.Disagreeing with an authority figure on a major issue. (S) 
8.Betting a day’s income at a high-stake poker game. (F/G)  
9.Having an affair with a married man/woman. (E) 
10.Passing off somebody else’s work as your own. (E) 
11.Going down a ski run that is beyond your ability. (R) 
12.Investing 5% of your annual income in a very speculative stock. (F/I) 

24 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
13.Going whitewater rafting at high water in the spring. (R)  
14.Betting a day’s income on the outcome of a sporting event  (F/G) 
15.Engaging in unprotected sex. (H/S) 
16.Revealing a friend’s secret to someone else. (E) 
17.Driving a car without wearing a seat belt. (H/S)  
18.Investing 10% of your annual income in a new business venture. (F/I)  
19.Taking a skydiving class. (R)  
20.Riding a motorcycle without a helmet. (H/S)  
21.Choosing a career that you truly enjoy over a more secure one. (S) 
22.Speaking your mind about an unpopular issue in a meeting at work. (S) 
23.Sunbathing without sunscreen. (H/S) 
24.Bungee jumping off a tall bridge.  (R) 
25.Piloting a small plane. (R) 
26.Walking home alone at night in an unsafe area of town. (H/S) 
27.Moving to a city far away from your extended family. (S) 
28.Starting a new career in your mid-thirties. (S) 
29.Leaving your young children alone at home while running an errand. (E)  
30.Not returning a wallet you found that contains $200. (E)  
Note. E = Ethical, F = Financial, H/S = Health/Safety, R = Recreational, and S = Social. 
 
 

25 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
Toronto Alexithymia Scale 
Bagby, R. M., Parker, J. D. A. & Taylor, G. J. (1994). The twenty-item Toronto Alexithymia Scale-I. Item 
selection and cross-validation of the factor structure. Journal of Psychosomatic Research, 38, 23-32. 
 

26 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
Highly Sensitive Persons Scale (Pluess, 2018) 
Pluess, M., Assary, E., Lionetti, F., Lester, K. J., Krapohl, E., Aron, E. N., & Aron, A. (2018). Environmental 
sensitivity in children: Development of the Highly Sensitive Child Scale and identification of 
sensitivity groups. Developmental psychology, 54(1), 51. 
 
 
1. I find it unpleasant to have a lot going on at once  
2. Some music can make me really happy 
3. I love nice tastes  
4. Loud noises make me feel uncomfortable 
5. I am annoyed when people try to get me to do too many things at once 
6. I notice it when small things have changed in my environment 
7. I get nervous when I have to do a lot in little time  
8. I love nice smells 
9. I don’t like watching TV programs that have a lot of violence in them 
10. I don’t like loud noises 
11. I don’t like it when things change in my life 
12 When someone observes me, I get nervous. This makes me perform worse than normal. 
 
 

27 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
The Positive and Negative Affectivity Scale Questionnaire (PANAS; Watson, Clark, & Tellegan, 1988)  
Watson D., Clark, L. A., & Tellegan, A. (1988). Development and validation of brief measures of positive 
and negative affect: The PANAS scales. Journal of Personality and Social Psychology, 54(6): 1063-
1070. 
 
This scale consists of a number of words that describe different feelings and emotions. Read 
each item and then list the number from the scale below next to each word.  Indicate to what 
extent you feel this way right now, that is, at the present moment OR indicate the extent you 
have felt this way over the past week (circle the instructions you followed when taking this 
measure) 
 
Very Slightly or Not at All 
   A Little 
   Moderately       Quite a Bit             Extremely 
 
           1 
        2  
          3                          4                           5 
_______ 1. Interested  
 
 
_______ 11. Irritable 
_______ 2. Distressed 
 
 
_______ 12. Alert 
_______ 3. Excited 
 
 
 
_______ 13. Ashamed 
_______ 4. Upset 
 
 
 
_______ 14. Inspired 
_______ 5. Strong 
 
 
 
_______ 15. Nervous 
_______ 6. Guilty 
 
 
 
_______ 16. Determined 
_______ 7. Scared 
 
 
 
_______ 17. Attentive 
_______ 8. Hostile 
 
 
 
_______ 18. Jittery 
_______ 9. Enthusiastic 
 
 
_______ 19. Active 
_______ 10. Proud 
 
 
 
_______ 20. Afraid 
 
 
 

28 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
Tybur 3 Domain Disgust Questionnaire (Tybur, 2009) 
Tybur, J. M., Lieberman, D., & Griskevicius, V. (2009). Microbes, mating, and morality: Individual 
differences in three functional domains of disgust. Journal of Personality and Social 
Psychology, 97: 103–122. 

29 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
Revised SocioSexual Orientation Inventory (Penke & Asendorpf, 2008) 
Penke, L., & Asendorpf, J. B. (2008). Beyond global sociosexual orientations: A more differentiated look 
at sociosexuality and its effects on courtship and romantic relationships. Journal of Personality 
and Social Psychology, 95, 1113-1135.  
 
 
 

30 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
Mental Health Measures 
Patient Health Questionnaire (PHQ-9; Kroenke & Spitzer, 2002)  
Kroenke K., Spitzer R.L. (2002). The PHQ-9: a new depression and diagnostic severity measure. Psychiatr 
Ann, 32: 509-21.  
(Note: The question about suicide has been deleted). 
Over the last 2 weeks, how often have you been bothered by any of the following problems? (0 = not at 
all; several days = 1; More than half the days = 2; Nearly every day = 3) 
1. Little interest or pleasure in doing things 
2. Feeling down, depressed, or hopeless 
3. Trouble falling or staying asleep, or sleeping too much 
4. Feeling tired or having little energy 
5. Poor appetite or overeating 
6. Feeling bad about yourself/failure 
7. Trouble concentrating on things 
8. Moving or speaking so slowly that other people could have noticed. Or being fidgety/restless. 
 
If you checked off any problems, how difficult have these problems made it for you to do your work, 
take care of things at home, or get along with other people? 
 
 
 

31 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
PAI-BOR (Morey, 1991) 
Morey, L. C. (1991). Personality Assessment Inventory: Professional Manual. Odessa, FL: Psychological 
Assessment Resources. 
 
 
Read each statement and decide if it is an accurate statement about you.  Circle your answer after each 
statement.  
 
False, not at all true – F    Slightly True – ST     Mainly True – MT    Very True - VT 
 
 
1.  My mood can shift quite suddenly.    
 
 
F 
ST 
MT 
VT 
2. My attitude about myself changes a lot.  
 
 
F 
ST 
MT 
VT 
3. My relationships have been stormy.  
 
 
F 
ST 
MT 
VT 
4. My moods get quite intense.   
 
 
 
F 
ST 
MT 
VT 
5. Sometimes I feel terribly empty inside. 
 
 
F 
ST 
MT 
VT 
6. I want to let certain people know how much they’ve hurt me.  F 
ST 
MT 
VT 
7. My mood is very steady.   
 
 
 
 
F 
ST 
MT 
VT 
8. I worry a lot about other people leaving me.    
 
F 
ST 
MT 
VT 
9. People once close to me have let me down.  
 
F 
ST 
MT 
VT 
10. I have little control over my anger.  
 
 
F 
ST 
MT 
VT 
11. I often wonder what I should do with my life.   
 
F 
ST 
MT 
VT 
12. I rarely feel very lonely.  
 
 
 
 
F 
ST 
MT 
VT 
13. I sometimes do things so impulsively that I get into trouble.  F 
ST 
MT 
VT 
14. I’ve always been a pretty happy person. 
 
 
F 
ST 
MT 
VT 
15. I can’t handle separation from those close to me very well.   F 
ST 
MT 
VT 
16. I’ve made some real mistakes in the people I’ve picked as friends.  F ST 
MT 
VT 
17. When I’m upset, I typically do something to hurt myself.   
F 
ST 
MT 
VT 
18. I’ve had times when I was so mad I couldn’t do enough to express my anger. F 
ST 
MT VT 
19. I don’t get bored very easily. 
 
 
 
F 
ST 
MT 
VT 
20. Once someone is my friend, we stay friends.   
 
F 
ST 
MT 
VT 
21. I’m too impulsive for my own good.   
 
 
F 
ST 
MT 
VT 
22. I spend money too easily.    
 
 
 
F 
ST 
MT 
VT 
23. I’m a reckless person. 
 
 
 
 
F 
ST 
MT 
VT 
24. I’m careful about how I spend my money. 
 
 
F 
ST 
MT 
VT 
 
 
 
 

32 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
Family Questionnaire (Felitti, 1997) 
Felitti, V. J., Anda, R. F., Nordenberg, D., Williamson, D. F., Spitz, A. M., Edwards, V., ... & Marks, J. S. 
(1997). Relationship of childhood abuse and household dysfunction to many of the leading 
causes of death in adults: The Adverse Childhood Experiences (ACE) Study. American journal of 
preventive medicine, 56(6), 774-786. 
 
 
Please answer these questions recognitioning back to your childhood using the scale: 
1 
2 
3 
4 
5 
Not at All 
 
 
 
Very Often 
 
1. How often did a parent or other adult in the household make you feel that you were loved, supported 
and cared for? 
2. How often did a parent or other adult in the household swear at you, insult you, put you down, or act  
3. How often did a parent or other adult in the household express physical affection for you, such as  
4. How often did a parent or other adult in the household push, grab, shove, or slap you? 
5. How often would you say that a parent or other adult in the household behaved violently toward a  
6. How often would you say there as quarreling, arguing, or shouting between your parents? 
7. How often would you say there was quarreling, arguing, or shouting between you’re a parent and you? 
8. How often would you say there was quarreling, arguing, or shouting between a parent and one of  
9. How often would you say there was quarreling, arguing, or shouting between your sibling(s) and you? 
10. Would you say the household you grew up in was chaotic and disorganized? 
11. In your childhood, did you live with anyone who was a problem drinker or alcoholic, or who used street 
drugs? 
12. Would you say that the household you grew up in was well-organized and well-managed? 
13. Would you say you were neglected while you were growing up, that is, left on your own to fend for 
yourself? 
 
 

33 
Study Design and Analysis Plan: Aspirin Effects on Emotional Reactions, NCT04146532, April 28, 2020 
Perceived Stress Scale (Cohen, Kamarck, & Mermelstein, 1983) 
Cohen, S., Kamarck, T., & Mermelstein, R. (1983). A global measure of perceived stress. Journal of  
health and social behavior, 385-396. 
 
 
 
0 = Never 
1= Almost Never 
2=Sometimes  
3=Fairly Often  
4=Very Often 
In the last month: 
1. How often have you been upset because of something that happened unexpectedly? 
2. How often have you felt that you were unable to control the important things in your life? 
3. How often have you felt nervous and “stressed”? 
4. How often have you felt confident about your ability to handle your personal problems? 
5. How often have you felt that things were going your way? 
6. How often have you found that you could not cope with all the things you had to do? 
7. How often have you been able to control irritations in your life? 
8. How often have you felt that you were on top of things? 
9. How often have you been angered because of things that were outside of your control? 
10. How often have you felt difficulties were piling up so high that you could not overcome them?