The Role of Matrix Metalloproteinase 2 and 9 Enzymes in Developing Chronic Thromboembolic Pulmonary Hypertension
Conditions: Chronic Thromboembolic Pulmonary Hypertension
Sponsor: AHMET ZENGIN

 
 
 
 
The Role of Matrix Metalloproteinase 2 and 9 Enzymes in Developing 
Chronic Thromboembolic Pulmonary Hypertension 
(NCT04773028) 
 
Date: 3 March 2020 

 
 
 
 
 
 
 
Study Protocol 
The study was planned prospectivly. 48 patients were included in the study by performing power 
analysis.. Groups were divided into two equal parts.24 cases(Group 1) and 24 controls (Group 2). 
 
The first group is the case group, patients who underwent pulmonary thromboendarterectomy due 
to CTEPH. From these patients, blood samples were taken into two blood tubes before starting the 
operation in the operating room, and 5 cm3 samples were taken from the endarterectomy material 
removed from the pulmonary artery during surgery. These patients are the patients who will 
undergo pulmonary endarterectomy operation. Patients who required other surgical interventions 
other than PTE such as aortic valve replacement and coronary bypass were not included in the first 
group. Patients with coronary carotid and peripheral arterial disease were not included. 
 
The second group is the control group, patients who underwent pneumonectomy or lobectomy for a 
reason other than CTEPH. The safe pulmonary arteries from the diseased lung segment removed 
during surgery were taken. Patients with high pulmonary artery pressure were not included in the 
second group. 
 
The collected tissues were transported in nitrogen tanks and stored in -80 degrees refrigerators.. 
Serum obtained by centrifugation of blood at 4000 rpm were stored in -80 degrees refrigerators. 
 
Mmp2 and Mmp 9 enzyme concentrations in serum and tissue were studied by western blot and 
eliza tests.