Urine Color Expressed in CIE L*a*b* Colorspace During Rapid Changes in Hydration Status
Phase: NA
Conditions: Dehydration
Sponsor: San Diego State University

Dec 13, 2021 
Using Urine Color as a marker of hydration status 
 
 
Study Protocol and Statistical Analysis Plan  
 
The study protocol consists of a crossover design. During one arm the subjects will report euhydrated 
and then dehydrate 2% by walking on a treadmill in the heat. During the second arm, subjects will report 
dehydrated after an overnight fast, and then rehydrate by drinking water ad lib over 2 hours. Urine 
specific gravity, urine osmolality, subjective urine color and objective urine color (expressed in CIELab 
space) with be collected at the start and end of each arm of the study. Paired t-tests will be used to 
determine if significant changes occur in each physiological variable during each of the 2 arms. Overall 
study significance will be set to p<0.05