Identifying Neurobiological Mechanisms That Underlie Acute Nicotine Withdrawal and Drive Early Relapse in Smokers
Phase: NA
Conditions: Nicotine Dependence
Sponsor: National Institute on Drug Abuse (NIDA)

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
 
 
 
 
 
 
page 1 of 221 
 
 
 
 
 
 
 
Protocol 12-DA-N474: Identifying neurological 
mechanisms that underlie acute nicotine withdrawal 
and drive early relapse in smokers. 
 
Approval Date: March 2, 2022 
 
NCT 01511614 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
 
 
 
 
 
 
page 2 of 221 
 
 
 
 
 
 
 
 
 
 
 
Protocol Title: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal 
and drive early relapse in smokers. 
 
 
Abbreviated Title: Neurobiology of nicotine withdrawal 
Protocol Number: 12-DA-N474 
Date of This Submission/Version: March 2, 2022 
 
Principal Investigator: 
Name, Degree 
Branch/Institute 
Bldg/Rm 
Phone/Fax 
Email 
Amy C. Janes, PhD 
NIDA/IRP//NRB 
BRC/07A709 
251 Bayview Blvd. 
Baltimore, MD 21224 
P: 443-740-2650 
F: 443-740-2734 
amy.janes@nih.gov  
 
Total requested accrual:  
Total Enrollees: 577 
Completers: 500 
Main Study:  
120 Treatment-seeking smokers (enrollees), 85 Completers 
42 non-treatment seeking smokers (enrollees), 35 completers. 
Motivational Interviewing Arm:  
300 people interested in quitting smoking, but not yet ready to set a quit date.  
Transcranial Direct Current Stimulation (tDCS) arm:  
60 non-treatment seeking smokers (enrollees), 35 completers 
55 nonsmokers (enrollees), 45 completers. Up to the first 25 of these enrollees will complete the tDCS without 
MRI (Behavioral Pilot) 
  
 
 
Project Uses Ionizing Radiation:    
 No   
 Yes  
 
 
 
 
 
 
IND/IDE 
 
 
 
 No   
Yes  
 
Durable Power of Attorney  
 
 No   
 Yes  
 
Multi-institutional Project 
 
 No   
 Yes  
 
 
 
 
 
 
NIDA is the coordinating site 
 
Data and Safety Monitoring Board  
 No   
 Yes  
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
 
 
 
 
 
 
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Technology Transfer Agreement  
 
 No   
 Yes  
 
MTA with LSU for plasma samples. Expires December 12, 2022. 
 
 
Samples are being stored  
 
 No   
 Yes (temporarily, plasma 
samples acquired for measurement of cortisol and ACTH will be destroyed once valid 
assays have been obtained. 
 
Flesch-Kincaid reading level of consent form:   7.4 
(exclude boilerplate in assessing reading level) 
 
Medical Coverage Level: 
Level 5 - The MAI (or designated clinician) is available by beeper or phone.  
 
** 
Level 1 - The MAI administers all drugs and remains physically present in the experimental room 
during all or part of study session as determined by the Clinical Director. 
Level 2 - The MAI must be physically present in the experimental room during all or part of 
study session as determined by the Clinical Director.  
Level 3 - The MAI must be present within the study building.  
Level 4 - The MAI must be on the study campus.  
Level 5 - The MAI is available by beeper or phone.  
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
 
 
 
 
 
 
page 4 of 221 
Précis: 
Objective 
The primary objective of the current protocol is to gain a greater understanding of the 
neurobiological mechanisms underlying acute nicotine withdrawal and contributing to the 
maintenance of, or return to smoking behavior among nicotine-dependent individuals, in the service 
of developing future smoking cessation treatments. The Nicotine Withdrawal Syndrome is a major 
cause of failed quit attempts in smokers, and targeting this time period for intervention may help 
improve smoking cessation outcomes. 
 
Study Population 
We will recruit treatment seeking and non-treatment seeking smokers, as well as matched non-
smoker control participants. 
 
Design 
There are 3 arms included in this protocol, each of which aims to understand the neurobiology of 
the Nicotine Withdrawal Syndrome during the initial quit period, with the broader goal of 
increasing quit success rate in the future. 
 
Main Study: To understand (1) the acute neurobiological effects of nicotine withdrawal 
on treatment-seeking and non-treatment seeking smokers, (2) the long term 
neurobiological outcomes of varenicline treatment and smoking cessation counseling at 1, 
6, and 12 months. We will recruit 85 treatment seeking and 35 non-treatment seeking 
smokers for a within (nicotine deprivation), between (treatment-seeking status) subjects 
randomized, double blind, placebo controlled study. 
 
Motivational Interviewing Arm: (1) To increase motivation and preparation for smoking 
cessation treatment among individuals who express an interest in quitting smoking but are 
not currently ready to enter treatment, in the service of increasing quit success rate and (2) 
to understand the neurobiological basis of motivation to quit smoking, and the interaction 
between motivation to quit and mechanisms that underlie acute nicotine withdrawal. We 
will recruit 300 current smokers interested in quitting smoking, but not yet ready to set a 
quit date. 
 
Transcranial Direct Current Stimulation (tDCS) Arm: To understand the acute effect of tDCS 
on 3 large-scale brain networks dysregulated in nicotine addiction and withdrawal, the Default 
Mode Network, the Executive Control Network, and the Salience Network. We will enroll 60 non-
treatment seeking smokers, with the expectation of 35 completers; and enroll 55 non-smoking 
controls, with the expectation of 45 completers, for a double blind, sham controlled, randomized 
crossover study. Smokers will be studied in nicotine abstinence and nicotine sated conditions, as in 
the Main Study design. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
 
 
 
 
 
 
page 5 of 221 
Outcome measures 
Primary outcome measures:  
1) Change in BOLD signal and FC related to task parameters, between drug (or tDCS) 
condition. 
2) Behavioral performance on each of the tasks assessing inhibitory control processes, reward 
responsiveness, amygdala, striatal, BNST reactivity, impulsive decision making, cue 
reactivity and working memory (e.g., reaction time, error rate, hit rate, reward bias). 
3) Self-reported craving, withdrawal symptoms and mood/affect 
4) Smoking abstinence as determined by self-reported tobacco use, urine cotinine, and breath 
CO. 
 
Secondary outcome measures: 
1) MRS for glutamate concentration. 
2) Plasma ACTH and cortisol. 
3) Resting state CBF from ASL. 
4) ERP and EEG measures. 
5) Ratings and scores on self-report characterization measures. 
6) Structural MRI and DTI data. 
7) Resting state FC at 1, 6 and 12 months post-treatment. 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
 
 
 
 
 
 
page 6 of 221 
Table of Contents 
Précis: ................................................................................................................................. 4 
Objective .................................................................................................................... 4 
Outcome measures ..................................................................................................... 5 
List of Abbreviations ........................................................................................................ 10 
1. Introduction/Scientific Rationale.................................................................................. 11 
1.1. 
Anhedonia during acute nicotine withdrawal: neurobiological mechanisms 12 
1.2. 
Stress reactivity and negative affect during acute nicotine withdrawal: 
neurobiological mechanisms ................................................................................................ 13 
1.3. 
The Brain’s Default Mode Network: Does it play a role in acute nicotine 
withdrawal? 14 
1.4. 
Inhibitory control and impulsive decision making during acute nicotine 
withdrawal: potential neurobiological mechanisms.............................................................. 15 
1.5. 
Motivation to quit .......................................................................................... 20 
1.6. 
Transcranial Direct Current Stimulation ....................................................... 20 
1.7. 
Considerations when conducting nicotine-withdrawal research ................... 21 
2. Study objectives or hypotheses .................................................................................... 23 
Overview ................................................................................................................. 23 
Study population ...................................................................................................... 23 
Design ...................................................................................................................... 23 
2.1. 
Primary goals ................................................................................................. 23 
2.2. 
Secondary goals ............................................................................................. 24 
2.3. 
Aims and hypotheses ..................................................................................... 24 
3. Subjects: ....................................................................................................................... 27 
3.1. 
Study population ............................................................................................ 27 
3.2. 
Inclusion criteria ............................................................................................ 27 
3.3. 
Exclusion criteria ........................................................................................... 29 
4. Study Design and Methods: ......................................................................................... 31 
4.1. 
Study overview .............................................................................................. 31 
4.2. 
Recruitment ................................................................................................... 33 
4.3. 
Screening methods ......................................................................................... 35 
4.4. 
Study procedures ........................................................................................... 37 
4.5. 
End of participation ....................................................................................... 49 
4.6. 
Relationship to other protocols. ..................................................................... 49 
5. Storage of data and samples ......................................................................................... 49 
6. Data Sharing Plan ......................................................................................................... 50 
7. Additional Considerations ............................................................................................ 50 
8. Risks/ discomforts ........................................................................................................ 50 
8.1. 
Questionnaire and Characterization Measures. ............................................. 50 
8.2. 
Nicotine Patch Administration. ..................................................................... 50 
8.3. 
Varenicline administration. (Applicable to treatment-seeking smokers only)
 
51 
8.4. 
Smoking abstinence/nicotine deprivation ...................................................... 52 
8.5. 
Blood draw .................................................................................................... 53 
8.6. 
Intravenous Catheter ...................................................................................... 53 
8.7. 
MRI Scanning. ............................................................................................... 53 
8.8. 
EEG Recording. ............................................................................................. 54 
8.9. 
Physiological monitoring. .............................................................................. 54 
8.10. Tasks completed inside and outside of the scanner. ...................................... 54 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
 
 
 
 
 
 
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8.11. Presentation of smoking-related pictures....................................................... 54 
8.12. Self-report characterization and state measures ............................................ 54 
8.13. Electric shock. ............................................................................................... 55 
8.14. Electric shock within the MRI scanner. ......................................................... 55 
8.15. Acoustic Noise. .............................................................................................. 55 
8.16. Mock Scanner. ............................................................................................... 56 
9. Subject monitoring ....................................................................................................... 56 
9.1. 
Parameters to be monitored ........................................................................... 56 
9.2. 
Criteria to be used .......................................................................................... 58 
9.3. 
Criteria for individual subject withdrawal ..................................................... 58 
10. Outcome measures ..................................................................................................... 59 
10.1. Primary outcomes measures ........................................................................... 59 
10.2. Secondary outcome measures include: ........................................................... 59 
11. Statistical Analysis ..................................................................................................... 60 
11.1. Pre-processing................................................................................................. 60 
11.2. Data-Analysis ................................................................................................. 60 
11.3. Criteria for significance .................................................................................. 62 
11.4. Power analysis ................................................................................................ 62 
11.5. Accrual number request, taking into account screening/ dropouts ................. 64 
12. Human Subjects Protection ........................................................................................ 65 
12.1 Subject selection ............................................................................................ 65 
12.2 Justification for exclusion of children ........................................................... 66 
12.3 Justification for exclusion of vulnerable populations .................................... 66 
12.4 Safeguards for vulnerable populations .......................................................... 66 
12.5 Justification of sensitive procedures. ............................................................. 66 
12.6 Staff Participation .......................................................................................... 67 
13. Anticipated Benefit ..................................................................................................... 68 
14. Consent documents/Process ....................................................................................... 68 
15. Data and Safety monitoring ........................................................................................ 68 
16.1. Criteria for Study Cessation .......................................................................... 69 
16. Quality Assurance ...................................................................................................... 69 
17. Event Characterization and reporting to the IRB, Clinical Director (CD) and Sponsor
 ....................................................................................................................................................... 69 
18. Alternatives to participation or alternative therapies .................................................. 69 
19. Confidentiality ............................................................................................................ 70 
20.1. Medical Records. ........................................................................................... 70 
20.2. Research Records/Data. ................................................................................. 70 
20.3. Stored Samples. ............................................................................................. 70 
20.4. Special Precautions. ....................................................................................... 70 
20. Conflict of Interest/Technology Transfer ................................................................... 71 
21. Research and Travel Compensation ........................................................................... 71 
22. Motivational Interviewing Arm .................................................................................. 72 
23.1 Arm Title ......................................................................................................... 72 
23.2 Arm Background ............................................................................................. 73 
23.3. Arm Objective ................................................................................................ 73 
23.4 Number and type of subjects ........................................................................... 73 
23.5. Arm design and procedures ............................................................................ 74 
23.6 Storage of data ................................................................................................. 74 
23.7 Risks/discomforts ............................................................................................ 74 
23.8 Outcome measures/Statistical Analysis ........................................................... 75 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
 
 
 
 
 
 
page 8 of 221 
23.9. Human subjects protection ............................................................................. 75 
23.10. Anticipated benefit ........................................................................................ 75 
23.11 Consent documents/process, Data and safety Monitoring, Quality Assurance, 
Adverse event and unanticipated problem reporting, Alternatives to participation, 
Confidentiality, Conflict of Interest ...................................................................................... 75 
23.12 Research and Travel Compensation .............................................................. 75 
23. Transcranial Direct Current Stimulation (tDCS) Arm ............................................... 76 
24.1. Arm Title ........................................................................................................ 76 
24.2. Arm Background ............................................................................................ 76 
24.3. Arm Objective ................................................................................................ 76 
24.4. Number and type of subjects .......................................................................... 81 
24.5. Modified Inclusion/Exclusion Criteria for tDCS Study Arm ......................... 81 
24.6. Arm design and procedures ............................................................................ 85 
24.7 tDCS Behavioral Pilot ..................................................................................... 99 
24.8. fMRI tasks .................................................................................................... 100 
24.9. Storage of data and Data Sharing Plan ......................................................... 101 
24.10. Risks/discomforts ....................................................................................... 101 
24.11. Outcome measures ...................................................................................... 101 
24.12. Statistical Analysis ...................................................................................... 102 
24.13. Human subjects protection ......................................................................... 103 
24.14. Anticipated benefit ...................................................................................... 103 
24.15. Consent documents/process ........................................................................ 103 
24.16. Data and Safety Monitoring. Quality Assurance. Adverse event and 
unanticipated problem reporting.  Alternatives to participation. Confidentiality. Conflict of 
Interest. 
103 
24.17. Research and Travel Compensation. .......................................................... 103 
References ...................................................................................................................... 105 
24. Appendix 1: Participant Eligibility Checklists ......................................................... 125 
Eligibility Checklist for Protocol 474-Nontreatment ................................................ 125 
Screening ........................................................................................................................ 125 
Inclusion Criteria ........................................................................................................... 125 
(YES/NO) ....................................................................................................................... 126 
Medical ........................................................................................................................... 126 
Inclusion criteria ............................................................................................................ 126 
Exclusion criteria ........................................................................................................... 126 
Eligibility Checklist for Protocol 474-treatment ....................................................... 128 
Screening ........................................................................................................................ 128 
Inclusion Criteria ........................................................................................................... 128 
(YES/NO) ....................................................................................................................... 129 
Medical ........................................................................................................................... 129 
Inclusion criteria ............................................................................................................ 129 
Exclusion criteria ........................................................................................................... 130 
Eligibility Checklist for Protocol 474-tDCS – Main Arm .............................................. 133 
Screening .............................................................................................................. 133 
Medical ................................................................................................................. 134 
Eligibility Checklist for Protocol 474-tDCS – Pilot ....................................................... 137 
Screening .............................................................................................................. 137 
Medical ................................................................................................................. 138 
Appendix 2: Participant Fact Sheets ............................................................................... 142 
STUDY  474 Treatment Seekers FACT SHEET ................................................................ 142 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
 
 
 
 
 
 
page 9 of 221 
STUDY  474 Non-treatment Seekers FACT SHEET ......................................................... 143 
STUDY  474 tDCS FACT SHEET for PARTICIPANTS WHO SMOKE CIGARETTES ............. 145 
STUDY  474 tDCS FACT SHEET for NONSMOKING PARTICIPANTS ................................ 146 
STUDY  474 tDCS BEHAVIORAL PILOT FACT SHEET ....................................................... 147 
Appendix 3: Characterization Measures ........................................................................ 148 
Appendix 4: State Measures (*administered during treatment sessions; #administered 
during scan sessions; + administered during follow-up scan sessions) ....................................... 180 
Appendix 5: Nicotine Patch Instructions ........................................................................ 199 
Appendix 6: Coping strategies ....................................................................................... 201 
Appendix 7: Varenicline Toleration Assessment ........................................................... 202 
Appendix 8: Take-home pill instructions ....................................................................... 203 
Appendix 9: Treatment Preparation and Motivation to Quit Measures ......................... 204 
Appendix 10: Treatment Materials ................................................................................. 213 
Appendix 11: Appointment Cards .................................................................................. 214 
Appendix 12: Nicotine Patch Instructions for tDCS Arm .............................................. 215 
Appendix 13: tDCS Stimulation Blinding Questionnaire .............................................. 218 
Appendix 14: Recruitment Materials ............................................................................. 219 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
 
 
 
 
 
 
page 10 of 221 
List of Abbreviations 
 
AChRs = acetylcholinergic receptors 
ACTH = Adrenocorticotropic hormone 
BLA = Basolateral Amygdala 
BNST = bed nucleus of the stria terminalis 
BOLD = blood oxygen level dependent 
CBF = cerebral blood flow 
CeA = Central Amygdala 
CRF = corticotrophin releasing factor 
dACC = dorsal anterior cingulate cortex 
dlPFC = dorsolateral prefrontal cortex 
DMN = Default Mode Network 
ECN = Executive Control Network 
ERN = error-related negativity 
ERP = event-related potential 
NAcc = Nucleus Accumbens 
PCC = posterior cingulate cortex  
Pe = Error Positivity 
PFC = prefrontal cortex 
PPC = posterior parietal cortex. 
rACC = rostral anterior cingulate cortex  
rsFC = resting state functional connectivity 
RT = reaction time 
SN = Salience network 
tDCS = Transcranial Direct Current Stimulation 
vmPFC = ventromedial prefrontal cortex 
VS = ventral striatal  
VTA = ventral tegmental area  
WM = Working memory  

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
 
 
 
 
 
 
page 11 of 221 
1. Introduction/Scientific Rationale 
 
Introduction 
In a review addressing smoking relapse rates following unassisted quitting attempts, Hughes, Keely 
and Naud (2004) found that while there is a decline in abstinence with time, 49% to 76% of smokers 
relapse within the first week. Thus, acute withdrawal processes appear to be the largest barrier to 
improving abstinence outcomes among nicotine-dependent individuals.  
 
Acute nicotine withdrawal is associated with reports of dysphoric or depressed mood, 
increased negative affect (anxiety, irritability, anger) and craving for cigarettes (American 
Psychiatric Association, 1994). Laboratory studies are consistent with these subjective reports, 
showing reduced reward and pleasure responsiveness, (Powell, Dawkins, & Davis, 2002), 
increased startle reactivity (Hogle, Kaye, & Curtin, 2010) and increased craving to drug cues 
(Powell, Dawkins, & Davis, 2002; Wang et al., 2007). In addition, acute nicotine withdrawal has 
been consistently shown to impair inhibitory control (McClernon, Kozink, Lutz, & Rose, 2009b; 
Pettiford et al., 2007; Powell et al., 2002) and increase impulsive decision making (Field, 
Santarcangelo, Sumnall, Goudie, & Cole, 2006a; Pettiford et al., 2007; Powell et al., 2002). This 
reduced responsiveness to natural rewards coupled with enhanced negative affect, impulsive 
decision making and poor inhibitory control are likely to significantly contribute to, and perhaps 
interact to compound, the risk of early relapse. Animal models point to neuroadaptations within the 
striatum, Ventral Tegmental Area (VTA) and extended amygdala as a possible neurobiological 
basis for acute withdrawal effects. However, to date, few studies have addressed the 
neurobiological mechanisms that underlie these processes, and drive early relapse in humans. 
 
The primary goal of the current research is to gain a greater understanding of the 
neurobiological processes underlying acute nicotine withdrawal and contributing to the 
maintenance of, or return to smoking behavior among nicotine-dependent individuals, in the service 
of developing future smoking cessation treatments.. We will address this goal by a 3-pronged 
approach.  
 
First, the Main Study of this protocol will examine the neurobiological correlates of 
affective and behavioral changes that occur during acute nicotine withdrawal and the extent to 
which these processes predict markers of risk for early relapse. A secondary consequence of this 
analysis will be the identification of neurobiological processes which underlie responsivity to 
nicotine replacement. The outcome of such an analysis is two-fold. Firstly, studying the 
neurobiological basis of acute nicotine withdrawal in humans may lead to the identification of novel 
treatment targets which could attenuate early relapse rates over and above that achieved by current 
therapeutic interventions. Secondly, by examining individual differences in responsivity to nicotine 
replacement we may be able to identify early biomarkers of poor treatment response which could 
guide clinicians in the selection of alternative interventions. To meet these goals, we will examine 
inhibitory control processes, impulsive decision making, extended amygdala and striatal reactivity, 
as well as drug cue and stress reactivity in nicotine dependent individuals under a non-deprived 
state and following 36 hrs of nicotine abstinence. 
 
Second, in the Motivational Interviewing Arm (Section 1.5), we will examine the effect of 
individual differences in motivation to quit smoking on the neurobiological outcome measures of 
the Main Study arm. Smokers vary widely in their motivation and preparation for smoking 
cessation treatment, and this may account for a portion of the discrepancy between the number of 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
 
 
 
 
 
 
page 12 of 221 
smokers who say they want to quit (which is high), and the number of smokers who successfully 
quit (which is low).  
 
Third, we will assess a new technology that has the potential to be used as a novel smoking 
cessation aid, Transcranial Direct Current Stimulation (tDCS). tDCS is a promising technique for 
treatment of cigarette smoking because of its ability to modify large scale brain networks that are 
dysregulated in nicotine addiction and withdrawal (see below, Sections 1.3, 1.4, 1.6). 
 
The following section provides background for our current understanding of the nicotine 
withdrawal syndrome, and how the 3 approaches described above together will bring further insight 
into the causes of quit attempt failures, and how the research community can develop treatments 
that will prevent those failures. 
 
Following this Introduction, and as per previous IRB requests, we have included all aspects of the 
tDCS arm in a discrete section of the protocol (see Section 24) 
1.1. Anhedonia during acute nicotine withdrawal: neurobiological mechanisms 
During acute withdrawal from nicotine, smokers typically report a state of anhedonia, 
defined as a reduced ability to experience pleasure from naturally rewarding activities/stimuli 
(Leventhal, Ramsey, Brown, LaChance, & Kahler, 2008).  This is consistent with laboratory studies 
showing reduced anticipation of pleasure from everyday rewarding activities (Powell et al., 2002) 
as well as reduced reward responsiveness across a variety of tasks involving monetary incentives 
(Al-Adawi & Powell, 1997; Dawkins, Powell, West, Powell, & Pickering, 2006; Powell et al., 
2002; Powell, Dawkins, West, Powell, & Pickering, 2010). High-levels of trait anhedonia and other 
depressive symptoms measured prior to quitting are in turn associated with poorer cessation 
outcomes and a greater number of previous quit attempts, in particular, greater relapse during the 
first 24 hours (Leventhal et al., 2008; Leventhal, Waters, Kahler, Ray, & Sussman, 2009).  
Preclinical studies show that acute withdrawal from nicotine results in reduced dopamine 
release at the nucleus accumbens (NAcc) and an increase in brain-reward thresholds (Hildebrand, 
Nomikos, Hertel, Schilström, & Svensson, 1998; Hildebrand, Panagis, Svensson, & Nomikos, 
1999). Studies suggest a role of corticotrophin releasing factor (CRF), in these effects (Bruijnzeel, 
Prado, & Isaac, 2009). During acute nicotine withdrawal, CRF increases in the brain and appears 
to act at the NAcc (Marcinkiewcz et al., 2009) to partially mediate elevations in brain-reward 
threshold.  
Neuroadaptations within glutamatergic systems have also been implicated in the anhedonic 
aspects of acute nicotine withdrawal. Nicotine acts at nicotinic acetylcholinergic receptors (AChRs) 
on glutamatergic terminals across a range of brain sites, including the VTA, NAcc, prefrontal cortex 
(PFC) and hippocampus (Kenny & Markou, 2001). Chronic exposure to nicotine leads to 
upregulation of inhibitory Glu2/3 receptors, producing a negative feedback effect on glutamatergic 
neurotransmission, particularly within the VTA (Kenny & Markou, 2001). Upregulation of 
inhibitory mGlu2/3 receptors during the development of nicotine dependence thus results in a 
reduction in glutamatergic neurotransmission during acute withdrawal, which has in turn been 
linked to an increased reward threshold during this period (Kenny & Markou, 2001). There is also 
evidence of reduced glutamatergic transmission from the PFC to NAcc during acute nicotine 
withdrawal that may contribute further to these reward deficits (Markou, 2008).  
Despite the link between depressive symptoms and early relapse to smoking, to date no 
study has examined the relationship between anhedonia produced by acute nicotine withdrawal and 
relapse outcomes. Furthermore, no study has examined the neurobiological basis of acute 
withdrawal induced anhedonia in smokers. Preclinical work implicates a CRF mediated reduction 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
 
 
 
 
 
 
page 13 of 221 
in the release of dopamine at the NAcc as well as reduced glutamatergic neurotransmission, 
particularly at the VTA and between the PFC and NAcc. These preclinical findings provide a 
starting point from which to identify the neurobiological mechanisms that underlie anhedonia 
following acute nicotine withdrawal in humans. Findings may also provide insight into the manner 
and extent to which these mechanisms contribute to the high rate of early relapse among smokers. 
1.2. Stress reactivity and negative affect during acute nicotine withdrawal: 
neurobiological mechanisms 
 
During acute withdrawal from drugs of abuse, neuroadaptations occur in the extended 
amygdala (the central nucleus of the amygdala [CeA], bed nucleus of the stria terminalis [BNST], 
and a transitional zone in the shell of the NAcc) which have been tied to the increase in negative 
affect and stress reactivity during this period (Koob and Le Moal, 2008). High negative affect and 
stress reactivity are in turn linked to increased risk for relapse, particularly in response to stress 
(Fisher et al., 1998; Willinger et al., 2002; Bottlender and Soyka, 2005; Cosci et al., 2009; Back et 
al., 2010). For example, mice bred for heightened stress reactivity show heightened reinstatement 
of nicotine seeking following extinction (Bilkei-Gorzo et al., 2008). 
Acute nicotine-withdrawal produces a substantial elevation in extracellular CRF at the CeA 
as well as an increase in anxiety-like behavior (e.g., defensive burying) and nicotine self-
administration (SA) following abstinence (George et al., 2007). Systemic injection of a CRF1 
receptor antagonist has been found to block these nicotine-withdrawal related anxiogenic effects 
and dose-dependently reduce abstinence induced nicotine SA (George et al., 2007). In the same 
study, direct infusion of CRF into the CeA produced the same anxiogenic effects in naïve animals. 
These findings suggest that the anxiogenic effects of nicotine withdrawal are at least partly 
mediated by the actions of CRF at CRF1 receptors and may be specifically related to an increase in 
CRF at the CeA.  
Whether CRF is elevated at other brain regions during nicotine-withdrawal is unknown. 
Previous studies have shown in increase in extracellular CRF at the BNST during withdrawal from 
alcohol (Olive, Koenig, Nannini, & Hodge, 2002). While action of CRF at the BNST does not 
appear to mediate the increased reward threshold during nicotine withdrawal (Marcinkiewcz et al., 
2009), studies with other drugs of abuse suggest a role for the BNST in negative affective aspects 
of acute nicotine withdrawal. For example, studies with alcohol and cocaine dependent rats 
implicate the BNST in stress-induced reinstatement of cocaine/alcohol seeking (Leri et al., 2002; 
Wang et al., 2006). A role of the BNST is also suggested by recent study showing enhancement of 
startle reflex under the threat of unpredictable but not predictable shock during nicotine withdrawal 
(Hogle et al., 2010). Threat of unpredictable shock produces a state of sustained anxiety mediated 
by the BNST in rodents (Davis et al., 2010) and shown to activate the BNST and CeA in humans 
(Alvarez et al., 2011).  
Individual differences in stress reactivity and trait anxiety in humans have been tied to 
amygdala reactivity in response to conscious and unconsciously processed emotional 
stimuli/stressors (Etkin et al., 2004; Gianaros et al., 2008; Monk et al., 2008). Amygdala reactivity 
in part reflects the degree of top-down regulation by rostral anterior cingulate regions (rACC) 
(Etkin, Egner, & Kalisch, 2010). Functional and structural connectivity between the rACC and 
amygdala have also been linked to trait anxiety/stress reactivity (Kim & Whalen, 2009; Pezawas et 
al., 2005). Both amygdala reactivity and amygdala-rACC connectivity are modulated by a common 
polymorphism in the human serotonin transporter gene (5-HTTLPR) (Caspi, Hariri, Holmes, Uher, 
& Moffitt, 2010; Pezawas et al., 2005). Serotonin is also heavily involved in the development of 
neural pathways that support emotion processing and regulation (Caspi et al., 2010). Individuals 
with the short allele of the 5-HTTLPR gene show lower expression of 5-HTT and enhanced 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
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reactivity to stress and risk for depression, effects which appear to be mediated by enhanced 
amygdala reactivity and amygdala-rACC connectivity (Caspi et al., 2010; Pezawas et al., 2005). 
Amygdala reactivity and regulation of amygdala output by the rACC could thus play a role in the 
negative affective aspects of acute nicotine withdrawal. 
To summarize, acute nicotine withdrawal is associated with enhanced reactivity to stress 
and a general increase in negative affect. At present, the neurobiological mechanisms underlying 
these effects in humans are relatively unknown. Preclinical work suggests that neuroadaptations 
within the extended amygdala may play a key role. As well, increases in central CRF during acute 
nicotine withdrawal and in particular the action of CRF at the CeA and BNST present potential 
mechanisms of interest, particularly in relation to stress-induced relapse. In humans, stress 
reactivity has been tied to enhanced amygdala reactivity and reduced top-down regulation of the 
amygdala by the rACC. These effects are both in turn modulated by a common polymorphism in 
the 5-HTTLPR gene. In the current study we thus aim to draw upon this body of research to identify 
key mechanisms underlying enhanced reactivity to stress and negative affect during acute nicotine 
withdrawal. We will utilize validated methods to probe reactivity in the amygdala and BNST as a 
function of acute nicotine withdrawal and self-reported negative affective symptoms, and also 
examine the extent to which these effects are mediated or modulated by the presence of the short 
allele of the 5-HTTLPR gene and a marker of central CRF. 
 
1.3. The Brain’s Default Mode Network: Does it play a role in acute nicotine 
withdrawal? 
 
As reflected above, current understanding of the neurobiological mechanisms underlying 
acute nicotine withdrawal is based almost exclusively on preclinical research. Such preclinical 
work directs focus to discrete neural regions where neuroadaptations may be driving withdrawal 
processes. However, more recently human neuroimaging has seen an explosion of interest in 
intrinsic functional networks that show state and trait alterations across a range of clinical 
populations (Broyd et al., 2009; Sakoğlu et al., 2011). In terms of acute nicotine withdrawal, 
emerging evidence points to a central role of a set of functionally connected structures collectively 
termed the default mode network (DMN). Regions included in the DMN typically include the 
ventromedial PFC, posterior cingulate cortex (PCC), inferior frontal lobe, dorsomedial PFC, 
parahippocampal gyrus and hippocampal formation (Buckner, Andrews-Hanna, & Schacter, 2008). 
DMN regions were originally identified because they were consistently shown to deactivate 
(relative to resting baseline) during goal-directed tasks (Buckner et al., 2008; Greicius, Krasnow, 
Reiss, & Menon, 2003; D. A. Gusnard & Raichle, 2001). These regions also exhibit robust 
functional connectivity at rest (Buckner et al., 2008; Greicius et al., 2003) and during task 
(Sridharan, Levitin, & Menon, 2008). 
The function of the brain’s DMN is still in question, however, most evidence points to a 
role in mediating internal thought related to mental simulation (e.g., rumination on past and future 
events, others thoughts, moral dilemmas) and self-reflection (Amodio & Frith, 2006; Buckner et 
al., 2008; Gusnard, Akbudak, Shulman, & M E Raichle, 2001; Schacter, Addis, & Buckner, 2007).  
Interestingly, resting connectivity within, and cerebral blood flow to, the DMN have been shown 
to increase as a function of nicotine withdrawal symptoms and cigarette cravings (Cole et al., 2010; 
Wang et al., 2007). Moreover, independent of withdrawal, increased activity in default mode 
regions is consistently observed as a function of craving and/or reactivity to drug cues (e.g., 
Franklin et al., 2011; Goudriaan, De Ruiter, Van Den Brink, Oosterlaan, & Veltman, 2010; Li, 
Chiang-Shan et al., 2008; Park et al., 2007; Sinha & C. S. R. Li, 2007; Weinstein et al., 2010; 
Wilcox, Teshiba, Merideth, Ling, & Mayer, 2011).  

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Taken together, this evidence suggests that craving and negative affective symptoms 
associated with acute nicotine withdrawal may produce a state of rumination/self-reflection 
mediated by the DMN. This view is consistent with enhanced resting connectivity and reduced 
task-related deactivation in default mode regions seen among clinically anxious and depressed 
populations (Gentili et al., 2009; Lanius, Bluhm, Coupland, Hegadoren, & Rowe, 2010; Sheline, 
Price, Yan, & Mintun, 2010; Zhou et al., 2010) as well as with the substantial overlap in DSM-IV 
symptoms of major depressive disorder (MDD) and acute nicotine withdrawal (APA, 1994). 
Increased engagement of the DMN may in turn contribute to the cognitive deficits observed 
during acute nicotine withdrawal (e.g., Myers, Taylor, Moolchan, & Heishman, 2008). During 
goal-directed tasks and at rest the DMN is negatively coupled with a functionally distinct executive 
control network (ECN), including dorsal frontal and parietal regions typically engaged during goal-
directed tasks and problem solving (Buckner et al., 2008; Fox et al., 2005; Fransson, 2005; 
Hampson, Driesen, Roth, Gore, & Constable, 2010). Consequently, reduced task-related 
deactivation in default mode regions predicts subsequent performance errors as well as reduced 
activity in ECN regions (Eichele et al., 2008; Li, Yan, Bergquist, & Sinha, 2007). 
To summarize, the brain’s DMN appears to mediate processes associated with self-
reflection and rumination on past and future events. Acute nicotine withdrawal is likely to produce 
an increase in rumination/self-reflection associated with cravings to smoke and negative affective 
processes. This will in turn produce enhanced connectivity/activity within the DMN at rest, reduced 
deactivation in default mode regions during task, and reduced engagement of the negatively 
coupled ECN during goal-directed task performance. This latter process may underlie observed 
deficits in sustained attention, performance monitoring and inhibitory control during acute nicotine 
withdrawal. According to this view, which is summarized in Figure 1, increased default mode 
activity/connectivity may reflect an intermediate endophenotype, driven by craving and negative 
affective processes during acute withdrawal, and resulting in impairments in cognitive functions 
and inhibitory control as well as increased risk of relapse.  
1.4. Inhibitory control and impulsive decision making during acute nicotine withdrawal: 
potential neurobiological mechanisms 
 
Deficits in inhibitory control mechanisms and impulsive decision making are considered 
central to drug addiction, which is characterized by compulsive use of drugs in the face of negative 
consequences (e.g., negative health outcomes). In the laboratory, inhibitory control deficits are 
revealed among nicotine-addicted individuals on tasks that require the inhibition of a prepotent 
response (Hester & Garavan, 2004). Nicotine-addicted individuals also tend to exhibit more 
impulsive decision making, characterized by sharper discounting of reward value (e.g., hypothetical 
money or cigarettes) as a function of time to delivery (Bickel, Odum, & Madden, 1999). More 
impulsive decision-making prior to quitting, as well as higher scores on trait impulsivity measures 
have in turn been linked to higher rates of relapse (Doran, Spring, McChargue, Pergadia, & 
Richmond, 2004; Kahler, Spillane, Metrik, Leventhal, & Monti, 2009; MacKillop & Kahler, 2009; 
Powell et al., 2010; Yoon et al., 2007).  
As outlined previously, inhibitory control deficits and impulsive decision making are 
exacerbated during acute nicotine withdrawal (Field et al., 2006a; McClernon et al., 2008a; 
Mitchell, 2004a; Pettiford et al., 2007; Powell et al., 2002), which likely contributes to the high rate 
of early relapse among smokers. However, to date, there has been no research addressing relapse 
outcomes as a function of acute-withdrawal induced changes in inhibitory control and impulsive 
decision making. Moreover, there is minimal understanding of the neurobiological processes 
driving these changes. Research addressing the increase in inhibitory control deficits and impulsive 
decision making during acute nicotine withdrawal, as well as the neurobiological basis of these 

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effects, may serve to identify a novel means of combating the high rates of early relapse among 
nicotine-addicted individuals. 
Inhibitory control processes. Breakdowns in inhibitory control may arise from poor monitoring for 
events (i.e., errors, goal-conflict) which signal the need to stop and adjust on-going behavior. They 
may also result from difficulty inhibiting/adjusting behavior in response to errors/conflict. It 
appears that these two components of inhibitory control are mediated by separable neurobiological 
mechanisms. The first, performance monitoring component, appears to be mediated by a network 
of structures centered around the dorsal region of the anterior cingulate cortex (dACC) and anterior 
insular cortex (Menon & Uddin, 2010; Seeley et al., 2007; Ullsperger, Harsay, Wessel, & 
Ridderinkhof, 2010). The two structures form part of an intrinsically connected “salience network” 
(SN) which is functionally distinct from other intrinsic brain networks including the DMN and 
ECN reviewed above. Within this network, the dACC monitors ongoing goal-directed behavior for 
salient events that require attention, while the anterior insula integrates high-level sensory, limbic, 
interoceptive and autonomic signals to generate subjective “feelings” which underlie awareness of 
salient intrinsic or extrinsic events (Menon & Uddin, 2010; Ullsperger et al., 2010).  
While the SN plays a critical role in monitoring on-going behavior for salient intrinsic or 
extrinsic events that require shifts in attention or cognitive/behavioral resources, it is not involved 
in later adjustment and conflict-resolution processes. When tasks are cognitive, the SN engages 
ECN structures, in particular the dlPFC, which function to resolve conflict/adjust behavior in 
response to conflict/errors (Botvinick, Nystrom, Fissell, Carter, & Cohen, 1999; Kerns et al., 2004; 
Marco-Pallarés, Camara, Münte, & Rodríguez-Fornells, 2008). In contrast, when tasks involve 
errors/conflict of an emotional nature, the salience network engages rACC regions of the DMN 
(Egner, Etkin, Gale, & Hirsch, 2008; Etkin, Egner, Peraza, Kandel, & Hirsch, 2006; Ochsner, 
Hughes, Robertson, Cooper, & Gabrieli, 2010). Interestingly, recent evidence suggests that the SN, 
in particular the anterior insula, functions as a switch between the negatively coupled DMN and 
ECN (Sridharan et al., 2008). 
 The anterior insula is also thought to play a key role in conscious urges, such as cigarette 
craving (Naqvi & Bechara, 2009). This view was compounded by reports of spontaneous smoking 
cessation and a striking loss of craving following damage to the insula (Naqvi, Rudrauf, Damasio, 
& Bechara, 2007). Imaging studies also consistently report insula activation as a correlate of 
subjective drug craving (Franklin et al., 2011; Naqvi & Bechara, 2009; Wang et al., 2007).   
Together, the above findings raise several potential mechanisms through which acute 
nicotine withdrawal may impair inhibitory control processes. Firstly, increased engagement of the 
DMN by craving/negative affective symptoms could reduce recruitment of the negatively coupled 
ECN during resolution of conflict. Secondly, previous findings (Eichele et al., 2008) suggest that 
reduced task-related DMN deactivation (e.g., during acute nicotine withdrawal) will result in 
reduced dACC engagement and hence impaired performance monitoring. Thirdly, if 
craving/negative affective symptoms functionally hijack the DMN, the capacity of these regions to 
effectively mediate conflict resolution/behavioral adjustment processes may also be altered. 
Finally, postulated involvement of the anterior insula in both craving and network switching raises 
the possibility that this region may play a role in enhanced DMN connectivity/activity during acute 
nicotine withdrawal.  
Evoked-potentials underlying inhibitory control processes. Further insight into the neural 
mechanisms which underlie breakdowns in inhibitory control may arise from the study of scalp-
recorded event-related potentials (ERPs) measured with electroencephalography (EEG). Much of 
the work reviewed above is based upon functional magnetic resonance imaging (fMRI) which 
produces a measure of blood oxygen level dependent (BOLD) activity in the brain. Although fMRI 
provides excellent spatial resolution, it is an indirect measure of underlying neuronal activity, with 
limited temporal resolution (typically sampling at 1Hz or slower). In contrast, ERPs are produced 
by the synchronous firing of neurons, and so provide a direct, real-time, measure of underlying 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
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neuronal activity. The measurement of ERPs is thus an important tool for dissociating processes 
following stimulus or response onset which would be otherwise indistinguishable within the BOLD 
response. 
Growing evidence indicates a dissociation between early negative (e.g., error-related 
negativity, ERN; N200) and late positive (error positivity, Pe; P300) ERP responses to errors and 
other salient events, respectively (Enriquez-Geppert, Konrad, Pantev, & Huster, 2010; Huster, 
Westerhausen, Pantev, & Konrad, 2010; Overbeek, Nieuwenhuis, & Ridderinkhof, 2005; 
Ullsperger et al., 2010). For example, children and young people with ADHD show normal ERN 
but reduced error positivity (Groom et al., 2010). They also show a reduction in late but not early 
evoked theta power in response to errors (Groom et al., 2010). Ullsperger et al. (2010) suggest that 
error negativity reflects error detection mediated by the dACC whereas error positivity reflects 
error awareness mediated by the anterior insula. Similarly, the N200 is thought to reflect early 
detection of conflict between expected and actual outcomes whereas later P300 responses are 
thought to reflect attentional allocation and evaluation of the salient event (Friedman, Cycowicz, 
& Gaeta, 2001). 
There is also evidence that synchronization in frontal scalp recorded theta oscillations may 
provide a correlate of increased connectivity between dACC and areas that mediate later 
control/conflict resolution processes (e.g., dlPFC). For example, (Cavanagh, Cohen, & Allen, 
2009) observed reduced theta power source localized to the dACC just prior to an error and 
increased theta power following an error. They also observed increased synchronization between 
medial frontal and lateral frontal electrode sites on error trials, which robustly predicted the degree 
of theta power on error trials. Furthermore, both theta synchronization and theta power predicted 
the degree of post-error reaction slowing (behavioral adjustment).  
There is now extensive evidence showing that each of the above negative and positive 
event-related components is sensitive to inhibitory control deficits observed in substance dependent 
populations and their high-risk offspring (e.g., H. L. Cohen, Porjesz, Begleiter, & W. Wang, 1997; 
Franken, van Strien, & Kuijpers, 2010; Luijten, van Meel, & Franken, 2011; Porjesz et al., 1998).  
In contrast, the impact of acute withdrawal on these ERP correlates is relatively unknown. By 
measuring concurrent ERP and fMRI correlates of performance monitoring and conflict 
resolution/behavior adjustment processes in the current study, we hope to gain further insight into 
the neurobiological basis of inhibitory control deficits observed during acute nicotine withdrawal.  
Impulsive decision making. Nicotine-addicted individuals tend to exhibit more impulsive 
decision making, characterized by sharper discounting of reward value (e.g., hypothetical money 
or cigarettes) as a function of time to delivery. More impulsive decision-making prior to quitting, 
as well as higher scores on trait impulsivity measures have in turn been linked to higher rates of 
relapse (MacKillop & Kahler, 2009; Yoon et al., 2007). Importantly, impulsive decision making is 
exacerbated during acute nicotine withdrawal (Field et al., 2006a; Pettiford et al., 2007; Powell et 
al., 2002). This in turn likely contributes to the high rate of early relapse among smokers. However, 
to date, there has been no research addressing relapse outcomes as a function of acute-withdrawal 
induced changes in impulsive decision making. Moreover, there is minimal understanding of the 
neurobiological processes driving these changes. Previous studies have shown that more impulsive 
decision making (steeper discounting as a function of delay to reward) is associated with enhanced 
striatal reactivity (Hariri et al., 2006) and reduced recruitment of default mode regions (Peters & 
Büchel, 2010). As outlined earlier, it is expected that acute nicotine withdrawal will be associated 
with reduced striatal reactivity. Given that more impulsive decision making results from enhanced 
striatal reactivity, we would expect reduced striatal reactivity to produce less impulsive decision 
making. Consequently, striatal changes are not expected to underlie impulsive decision making 
observed during acute nicotine withdrawal. Instead, it is expected that enhanced impulsive decision 
making during acute nicotine withdrawal will result from reduced recruitment of DMN regions, 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
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because these regions are otherwise occupied with rumination related to craving and affective 
symptoms. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in smokers.                                                                                                                                                 
 
                                                                                                                                                             Date: December 21, 2017 
Principal Investigator: Elliot A. Stein, Ph.D.                                                                                               Version:     9.0 
 
Figure 1. Summary of hypothesized neurobiological mechanisms underlying anhedonia, negative affect inhibitory control and impulsive 
decision making deficits experienced during acute nicotine withdrawal. The figure also outlines the proposed shift in resting state network (RSN) 
dynamics with thicker arrows illustrating increased input and connectivity during acute nicotine withdrawal relative to nicotine satiety. We also 
working memory (WM) and sustained attention deficits in our list of effects resulting from reduced engagement of the Executive Control Network 
(ECN) as these will be measured in the current protocol. DMN = Default Mode Network; SN = Salience network; VS = ventral striatal; BNST = 
bed nucleus of the stria terminalis; Amyg = amygdala; CRF = corticotrophin releasing factor; rACC = rostral anterior cingulate cortex; dACC = 
dorsal anterior cingulate cortex; rsFC = resting state functional connectivity; CBF = cerebral blood flow; BOLD = blood oxygen level dependent; 
ERP = event-related potential; RT = reaction time; ERN = error-related negativity; Glu = glutamate; VTA = ventral tegmental area; PCC = 
posterior cingulate cortex; vmPFC = ventromedial prefrontal cortex; dlPFC = dorsolateral prefrontal cortex; PPC = posterior parietal cortex. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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1.5.  Motivation to quit 
Motivation to quit smoking can have a profound effect on neural activation and craving elicited to drug 
cues (for reviews Wertz & Sayette, 2001; Wilson, Sayette, & Fiez, 2004b). For example, Wilson and colleagues 
(2004) observed that engagement of prefrontal cortical regions (OFC, dlPFC, dACC) in response to drug-related 
cues is primarily observed in non-treatment seeking smokers. When treatment-seeking smokers are examined, 
studies are much less likely to observe cue-induced engagement of prefrontal regions (Wilson, Sayette, & Fiez, 
2004a). The differential engagement of prefrontal regions in response to drug cues likely reflects differences 
between the groups in motivational/cognitive control processes (Wilson et al., 2004b), and in some cases, 
expectancy to smoke (McBride, Barrett, Kelly, Aw, & Dagher, 2006). To date, the large majority of neuroimaging 
studies on nicotine addiction are based on non-treatment seeking smokers. The observation of Wilson et al. (2004) 
and a recent study by this same group (Wilson, Sayette, & Fiez, 2012) raise the question of whether relapse- or 
abstinence-related effects observed in non-treatment seeking smokers can be applied to treatment-seeking 
smokers for whom this research is most pertinent. Moving forward it is therefore important to assess effects in 
both non-treatment and treatment seeking smoker populations to establish convergent and divergent mechanisms 
and to provide a greater understanding of how motivation to quit smoking interacts with relapse- and abstinent-
related processes.  
 
1.6. Transcranial Direct Current Stimulation 
 
Sections 1.1 to 1.5 of the introduction highlight the challenge of treating nicotine dependence. Cigarette 
smoking remains the leading cause of preventable death and disease in the United States, accounting for 480,000 
deaths each year (CDC 2016). Despite our improved understanding of the neurobiology of nicotine addiction and 
drug abuse over the past 10 years (Nestler 2005, Everitt and Robbins 2005, Koob and Volkow 2010, Goldstein 
and Volkow 2011), current pharmacological interventions for smoking cessation remain highly inadequate, with 
the most efficacious treatment (varenicline) having only about 27% absolute cessation rate according to recent 
meta-analysis (Cahill 2013, 2014). Additionally, symptoms of nicotine withdrawal remain a major impediment 
for smokers trying to quit, with most quit attempts failing within the first week of abstinence when withdrawal 
symptoms are at their height (Hughes 1992, Sutherland et al 2017). 
 
As introduced in Sections 1.3 (The Brain’s Default Mode Network) and 1.4 (Inhibitory Control & 
Impulsive Decision Making during Nicotine Withdrawal), recent findings based on resting state functional 
connectivity (rsFC) revealed that the brain can be divided into multiple large-scale functional networks, many of 
which have been found to contribute to psychiatric disease (Lu and Stein 2014, Turk-Browne 2013, Yeo et al 
2011, Fox et al 2014). Addiction has been hypothesized as an imbalance within and between three of these 
networks (Lerman et al 2014, Pariyadath et al 2015, Fedota and Stein 2015, Sutherland et al 2012). Specifically, 
the cognitive and affective disturbances of nicotine withdrawal syndrome have been attributed to a reduced 
coherence within-network connectivity of the Executive Control Network (ECN) and increased within-network 
connectivitystrength of the Default Mode Network (Sutherland et al 2017). Additionally, the Salience Network 
(SN) has been called the “gatekeeper” for these two networks, and its function may be dysregulated in addictive 
disorders, as well (Sutherland et al 2017). 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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A treatment that acts by modifying these large-scale brain networks with specificity may help address the 
underlying pathophysiology of nicotine addiction and improve clinical outcomes (Dunlop et al 2016). 
Transcranial Direct Current Stimulation (tDCS), a type of Non-invasive Brain Stimulation (NIBS), has the 
potential to modify neuronal circuits by application of a subthreshold conductive current through the scalp. Two 
potential targets for tDCS as a smoking cessation aid are the dorsolateral pre-frontal cortex (dlPFC), a node of 
the ECN, and the ventromedial prefrontal cortex (vmPFC), a node of the DMN (Lerman et al 2014). tDCS can 
potentially strengthen the control of the ECN through excitatory stimulation of the dlPFC, and weaken the 
influence of the DMN by inhibitory stimulation of the vmPFC. It is important to study the acute effects of tDCS 
applied to these networks to better understand its potential to be used as a smoking cessation aid in the future. 
1.7. Considerations when conducting nicotine-withdrawal research 
 
Nicotine Abstinence Period.  The affective (e.g., anhedonia, anxiety, irritability) and cognitive (e.g., 
difficulty concentrating) withdrawal symptoms following acute nicotine abstinence show an onset of 2 to 12 hrs 
post-cessation and peak within 2-3 days (J R Hughes, Higgins, & Bickel, 1994). Most prior imaging and EEG 
studies have assessed acute withdrawal effects following overnight abstinence (typically 8 to12 hrs of smoking 
deprivation; e.g., Azizian et al., 2010; Beaver et al., 2011; D. M. Cole et al., 2010; Loughead et al., 2010; Sweet 
et al., 2010) and a few, 24 hours abstinence (Kozink, Kollins, & McClernon, 2010a; McClernon et al., 2009b).  
 
 
Two different durations of the abstinence period have been selected for the Main Study Arm (36 hours 
deprivation) and the tDCS Arm (12 hours deprivation). Each has advantages and disadvantages, and the time 
periods selected best suit the individual aims within the particular arms. 
 
We have selected 36 hours for the Main Study Arm because it approximates maximal acute withdrawal 
effects, producing robust effects across MRI, EEG and behavioral measures that can be used as predictors for 
success on sustained abstinence later in the study design. This time period ensures that the study can capture the 
neurobiological changes and behavioral phenomena that underlie acute nicotine withdrawal effects. However, 
this long period of abstinence comes at the expense of subject compliance. 
 
Although a longer abstinence period accentuates the nicotine withdrawal effects, shorter periods of 
abstinence (such as 12 hours) have been found to result in changes in behavior, resting state functional 
connectivity, and BOLD patterns. Previous studies using overnight abstinence periods (10-12 hrs) have shown 
acute nicotine effects when assessing behavioral performance (e.g., Mancuso et al., 1999; Dawkins et al., 2006, 
2007b), EEG/ERPs (e.g., Knott et al., 1999; Pritchard et al., 2004; Gilbert et al., 2007), and fMRI signals (e.g., 
Xu et al., 2005; Brody, 2006; Wang et al., 2007). For example, using a battery of behavioral tests, acute smoking 
deprivation (12hrs) has been associated with impaired reward motivation (Dawkins et al., 2006) and a reduced 
capacity to inhibit prepotent motor responses (Dawkins et al., 2007b). With regard to fMRI signals, nicotine-
abstinent states (12hr), in comparison to satiated states, have been associated with significant regional cerebral 
blood flow increases in ACC during “resting” periods (Wang et al., 2007) and alterations in dorsal lateral PFC 
activity during working memory tasks (Xu et al., 2005).   
 
We have selected 12 hours abstinence for the tDCS Arm because we have shown it sufficient to achieve 
a “delta” in these outcome measures when compared to the non-deprived condition, allows us to test the 
hypotheses germane to this arm, and has the added benefit of improved subject compliance. The 12 hours 

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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abstinence will allow an evaluation of how tDCS can acutely modify large-scale networks in both the nicotine 
sated and nicotine deprived conditions.   
 
Individually Tailored Nicotine Dose. The purpose of the current study is to understand the neurobiological 
mechanisms underlying acute nicotine withdrawal in humans. Chronic nicotine administration in animals (Marks, 
Stitzel, & Collins, 1986) and humans (Breese et al., 1997) produces a dose-dependent increase in neural 
adaptations which ultimately underlie acute withdrawal effects (Buisson & Bertrand, 2002). In an effort to 
minimize nicotine withdrawal effects in the non-deprived condition it is therefore critical that we match each 
participant’s nicotine-patch dose to their typical daily nicotine intake (how? Who does this?). Individually tailored 
nicotine patch doses have been used in smoking cessation trials (Hurt, 2003) and higher doses have been shown 
to produce a greater reduction of withdrawal symptoms in heavier users (Ebbert et al., 2007) . In addition, NIDA 
protocol 398 also employs the use of an individually tailored nicotine dose based on the number of daily cigarettes. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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2. Study objectives or hypotheses 
 
Overview 
The Main Study will focus on the anhedonia, negative affect, inhibitory control deficits and impulsive decision 
making which accompany acute nicotine withdrawal in humans. The extent to which these acute withdrawal- 
related processes predict the success of smoking-cessation treatment will be assessed. The primary objective is to 
gain a greater understanding of the neurobiological mechanisms underlying acute nicotine withdrawal and 
contributing to the maintenance of, or return to smoking behavior among nicotine-dependent individuals. 
Secondary objectives are to understand the neurobiological basis of responsivity to nicotine replacement with 
respect to acute nicotine withdrawal symptoms, and to examine the neurobiological mechanisms underlying 
motivation to quit smoking and the interaction between motivation to quit and symptoms, which arise during 
acute withdrawal from nicotine. 
Study population  
We aim to recruit 85 nicotine-dependent individuals aged 18 to 55 years who are currently seeking treatment for 
smoking cessation (treatment-seeking smokers) and 35 nicotine-dependent individuals aged 18-55 years who are 
not currently seeking treatment for smoking cessation (non-treatment seeking smokers). Nicotine dependency 
will be based on a urine cotinine level corresponding to smoker status for the specific test being used, typically 
corresponding to a urine cotinine above about 200 ng/ml, and a history of smoking consistently for at least one 
year.  
Design 
The study will be a within (nicotine deprivation), between (treatment-seeking status) subjects double blind 
placebo controlled design. All participants will complete two scanning sessions, each preceded by a 36 hour 
smoking abstinence period. During one abstinence period and scanning session participants will wear a dose-
matched nicotine patch (non-deprived), and during the other abstinence period and scanning session, a placebo-
patch (nicotine-deprived). Following participation in both scan sessions treatment-seeking participants will 
commence 12 weeks of treatment involving daily varenicline administration with weekly counseling. Follow-up 
assessments will be conducted with treatment-seeking smokers at 1, 6 and 12 months after their last treatment 
visit. 
 
2.1. Primary goals 
To characterize the neurobiological mechanisms underlying acute nicotine withdrawal and the 
maintenance of smoking behavior in current cigarette smokers. To achieve this, the current protocol will employ 
multiple task-based and resting state measures to probe the anhedonic and anxiogenic effects of acute nicotine 
withdrawal as well as reported inhibitory control deficits and increased impulsive decision-making. Changes in 
RSN dynamics will also be examined in relation to affective and cognitive symptoms associated with acute 
nicotine withdrawal. Acute nicotine-withdrawal will be manipulated by assessing dependent smokers twice: once 
in a non-deprived state (following 36 hours of smoking abstinence during which time they will continually wear 
a dose-matched nicotine patch) and on a second occasion in a nicotine-deprived state (following 36 hours of 
smoking abstinence whilst wearing a placebo patch). In the nicotine-deprived condition smokers are expected to 
report anhedonia and negative affective symptoms and associated changes in mesolimbic dopaminergic pathways 

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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as well as amygdala and BNST functioning. As illustrated in Figure 1, we expect these changes to drive inhibitory 
control deficits and elevated impulsive decision making by altering functioning within and between intrinsic 
default mode, executive control and salience networks. Finally, the extent to which neurobiological changes 
resulting from acute nicotine withdrawal predict success of treatment for smoking cessation will be assessed. 
2.2. Secondary goals  
An additional aim of the current protocol is to identify a neurobiological basis for variance in responsivity 
to nicotine replacement.  Individual differences in response to nicotine replacement exist both in the degree of 
alleviation of acute withdrawal symptoms (Cole et al., 2010) as well as in terms of longer-term relapse outcomes 
(Uhl et al., 2008). In the current protocol responsivity will be operationalized as change in craving, affective and 
cognitive symptoms following nicotine-deprivation relative to nicotine-satiety with a dose-matched nicotine 
patch. By examining the source of individual differences in responsivity to nicotine replacement, we may be able 
to identify neurobiological targets for adjunct or alternative treatments in poor responders. We will also examine 
the extent to which pre-existing traits predict neurobiological correlates of responsivity to nicotine replacement. 
 
This protocol also aims to examine the common short/long allele polymorphism in the 5-HTTLPR gene 
as a mediator of acute nicotine withdrawal effects on amygdala reactivity and rACC-amygdala connectivity. 
 
A final goal of the present research is to examine the neurobiological mechanisms underlying motivation 
to quit smoking and the interaction between motivation to quit and symptoms which arise during acute withdrawal 
from nicotine. To achieve this aim, non-treatment seeking smokers will be recruited and will complete the same 
experimental procedures outlined in Section 2.1 under nicotine-deprived and non-deprived state but will not enter 
treatment for smoking cessation and will not be followed-up to assess for treatment success. We will examine the 
same processes outlined in Section 2.1 which we are assessing as a function of acute nicotine withdrawal and will 
look for between group differences (treatment-seeking vs non-treatment seeking smokers) on these measures. 
2.3. Aims and hypotheses 
Aim 1: To examine the effect of acute nicotine withdrawal on self-reported anhedonia and its relationship 
to reward responsiveness and striatal reactivity, and in turn the extent to which these withdrawal-related 
neurobiological processes predict treatment success. Acute nicotine withdrawal produces a state of anhedonia and 
reduced reward responsiveness. Preclinical work suggests that these effects may result from reduced dopamine 
release at the NAcc (manifesting in reduced striatal reactivity), mediated by increased central CRF and potentially 
by reduced glutamate signaling in a circuit from the PFC to the NAcc, and potentially the hippocampus. 
 
Measures: Self-reported anhedonia; behavioral and EEG indices of reward responsiveness; BOLD 
response and functional connectivity during striatal reactivity task; task measure of striatal reactivity, EEG and 
behavioral correlates of reward responsiveness.  
Mediators: Plasma ACTH; Glutamate concentration at the PFC/ACC and/or hippocampus measured 
with MRS. 
 
Hypothesis 1.1: Compared with nicotine satiety, acute nicotine withdrawal will result in increased self-
reported anhedonia, accompanied behavioral correlates of reward responsiveness and reduced ventral striatal 
reactivity. The degree of reduced striatal reactivity and reward responsiveness will in turn predict treatment 
success. 
 

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Hypothesis 1.2: The effect of acute nicotine withdrawal on striatal reactivity will be partially mediated 
by an increase in plasma adrenocorticotropic hormone (ACTH; an index of central CRF).  
 
Hypothesis 1.3: Acute nicotine withdrawal will result in reduced glutamate measured with MRS at the 
hippocampus and PFC, with the latter predicting reduced PFC-NAcc connectivity. Reduced PFC glutamate, as 
well as reduced PFC-NAcc signaling will partially mediate the predicted reduction in ventral striatal reactivity. 
 
Aim 2: To examine the effect of acute nicotine withdrawal on self-reported negative affect (anxiety, 
irritability) and stress reactivity, and in turn on reactivity in the amygdala and BNST. Acute nicotine withdrawal 
produces marked elevations in negative affect and enhanced stress reactivity. Although preclinical literature is 
limited, there is some evidence that these effects may be mediated by enhanced central CRF acting at the amygdala 
and BNST. Neuroimaging studies have linked individual differences in negative affect and stress reactivity to 
amygdala reactivity as well as top down regulation from the rostral ACC. Both amygdala reactivity and rACC-
amygdala connectivity are in turn mediated by the short/long allele polymorphism in the 5-HTTLPR gene.  
 
Measures: Self-reported negative affect (e.g., anxiety, irritability) and craving; BOLD response and 
functional connectivity during amygdala reactivity task and USCRT.  
Mediators: Plasma ACTH; 5-HTTLPR short/long allelic polymorphism 
 
Hypothesis 2.1: Compared with nicotine satiety, acute nicotine withdrawal will result in enhanced 
negative affect (e.g., anxiety, irritability), accompanied by enhanced amygdala and BNST reactivity. These effects 
will be partially mediated by an increase in plasma ACTH (as a marker of central CRF). 
 
Hypothesis 2.2: The effect of nicotine withdrawal on amygdala reactivity will be partially mediated by 
reduced regulatory input from the rACC. During nicotine withdrawal we expect the rACC (and other DMN 
regions) to be engaged in rumination on craving and affective symptoms. Consequently, we expect reduced task-
related regulatory input from the rACC to the amygdala, manifesting in reduced rACC-amygdala effective 
connectivity during an amygdala reactivity task.  
 
Hypothesis 2.3: The effect of acute nicotine withdrawal on amygdala reactivity and rACC-amygdala 
connectivity will be most pronounced among individuals expressing the short allele of the 5-HTTLPR gene. 
 
Hypothesis 2.4: Larger changes in amygdala and BNST reactivity will be associated with poorer 
treatment outcomes. 
 
Aim 3: To examine the neurobiological basis of inhibitory control deficits and impulsive decision making 
observed during acute nicotine withdrawal and the extent to which these processes predict treatment success. 
Inhibitory control deficits may arise from poor monitoring for salient events (errors, conflict) which signal the 
need to adjust behavior (insula-dorsal ACC network) or problems adjusting behavior following errors or conflict 
(ECN or rACC). During acute nicotine-withdrawal we expect reduced task-related DMN deactivation, driven by 
enhanced craving and cognitive symptoms. This should in turn result in reduced engagement of the dACC in 
extrinsic performance monitoring and reduced ECN and rACC engagement in post-conflict/error adjustment 
processes and decision-making. 
 

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Measures:  BOLD, ERP and behavior during go-nogo, affective flanker, and striatal reactivity tasks; 
behavior during the delay discounting task, Self-reported craving and affective symptoms, BOLD rsFC and CRF 
at rest. 
Mediators: Task-related BOLD 
 
Hypothesis 3.1: Relative to nicotine satiety, acute nicotine withdrawal will be associated with reduced 
dACC engagement in performance monitoring. Due to lower dACC engagement during on-going performance 
monitoring (e.g., congruent or go trials which typically function as a baseline), detection of errors or conflict will 
be associated with an enhanced dACC signal  and enhanced amplitude in ERP correlates of conflict detection. 
Behaviorally, reduced dACC engagement during performance monitoring will be mediated by reduced DMN 
deactivation, each of which will be associated with greater variance response times  and more performance errors.  
 
Hypothesis 3.2: Nicotine deprivation (relative to nicotine satiety) will be associated reduced DMN 
deactivation during task, will be greatest in individuals reporting more severe craving and affective symptoms. 
Reduced task-related deactivation in the DMN will in turn compromise the ability of the rACC (being part of the 
DMN) and the ECN (which is negatively coupled to the DMN) to engage in task-related conflict-resolution and 
post-error adjustment processes.  
 
Hypothesis 3.3: Acute nicotine withdrawal is expected to lead to a steeper discounting of rewards as a 
function of time (more impulsive decision making). More impulsive decision making will be associated with 
enhanced rsFC connectivity in the DMN and between the DMN and insula at rest, and greater CBF to DMN 
regions. We expect the relationship between impulsive decision making and rsFC and resting CBF changes 
following acute nicotine withdrawal to be strongest in medial PFC components of the DMN 
 
Aim 4: To examine the impact of 36 hrs of acute nicotine-withdrawal, relative to nicotine satiety, on rsFC 
and CBF within and between the DMN, ECN and salience network. We also wish to examine the relationship 
between these changes and affective and cognitive withdrawal symptoms, urge to smoke and treatment success. 
 
Measures: self-reported affective and cognitive withdrawal symptoms; self-reported urge to smoke; 
resting connectivity within and between the DMN, ECN and salience networks; CBF to DMN, ECN and salience 
network regions at rest. 
 
Hypothesis 4.1: Compared to nicotine satiety, acute nicotine withdrawal will be associated with enhanced 
resting state connectivity within the DMN and between the DMN and insula node of the salience network. The 
degree of elevation in resting connectivity within the DMN and between the DMN and insula will predict the 
change in self-reported urge to smoking, severity of affective withdrawal symptoms and treatment success. 
 
Hypothesis 4.2: Acute nicotine withdrawal will be associated with reduced connectivity within the ECN 
and between the ECN and insula, as well as reduced negative coupling between the ECN and DMN. The degree 
of reduced connectivity and negative coupling will predict with the severity of cognitive withdrawal symptoms 
as well as treatment success.  
 
Aim 5: To examine characteristics of rsFC associated with long-term abstinence following treatment for 
smoking cessation. To address this aim we will examine the following exploratory questions:   
 
Q1. What characteristics of rsFC are associated with treatment success (vs failure) at 1, 6 and 12 months 
post-treatment? 

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Q2. Are there characteristics of rsFC that vary as a function of time post-treatment in successfully 
abstinent individuals? 
 
Aim 6: To examine the neurobiological basis of motivation to quit smoking and the interaction between 
motivation to quit and mechanisms underlie acute nicotine withdrawal.  
Q1. Do the self-reported symptoms of acute nicotine withdrawal (e.g., anhedonia, anxiety/irritability, 
difficulty concentrating, impulsive decision making) and the putative neurobiological processes which underlie 
these (e.g., enhanced amygdala reactivity, reduced striatal reactivity, hypothesized shift in resting state network 
dynamics in Fig 1) vary as a function of motivation to quit (treatment-seeking vs non-treatment seeking smokers).  
Q2. Is there a main effect of motivation to smoke (treatment-seeking vs non-treatment seeking smokers) on 
measures of striatal and amygdala reactivity, cognitive control, drug cue reactivity and resting state network 
dynamics.  
3. Subjects: 
3.1. Study population 
Treatment-seeking smokers. This study will enroll healthy male and female adults who have a history of 
1 or more of consistently years smoking cigarettes with a urine cotinine level corresponding to smoker status for 
the specific test being used, typically corresponding to a urine cotinine above about 200 ng/ml, and are actively 
seeking treatment for smoking cessation. We are requesting to enroll a total of 120 treatment-seeking smokers, 
with 85 participants expected to a) complete the two pre-treatment scan visits; 2) set a quit date; 3) attempt to quit 
for at least 24 hours; 4) have their quit status established (i.e., relapsed vs abstinent) at 1, 6 and 12 months 
following their quit date. 
Non-treatment seeking smokers. This study will also recruit 42 healthy male and female adults who have 
a history of 1 or more years of consistently smoking cigarettes with a urine cotinine level corresponding to smoker 
status for the specific test being used, typically corresponding to a urine cotinine above about 200 ng/ml, but who 
are not currently seeking treatment for smoking cessation. 35 participants are expected to complete the two pre-
treatment scan visits. Non-treatment seeking smokers will not set a quit date or make a quit attempt, will not 
complete a treatment program as part of this study and will not be followed up after they complete the two pre-
treatment visits. 
3.2. Inclusion criteria 
All participants must:  
 
(1) Be between the ages of 18-55. Be right-handed. Assessment tool(s): Edinburgh Handedness Inventory. 
(2) Be in good health. Justification:  Many illnesses may alter fMRI signals as well as cognitive processes 
and neural functioning.  Assessment tool(s): Participants will provide a brief health history during phone 
screening, and undergo a medical history and physical examination with a qualified IRP clinician. 
(3) Be free of active DSM-IV dependence, or dependence in partial remission, on alcohol or any drug except 
nicotine. Past active dependence is acceptable provided it is at least five years in the past and total time 
of active dependence did not exceed 4 years. Those with past dependence on any substance other than 
alcohol or marijuana may not have any current use (past 6 months) of the substance on which they were 
dependent. For individuals with past alcohol or marijuana dependence, current use of the previously 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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dependent substance will be allowed providing they do not meet any current DSM-IV criteria for 
substance dependence, with the exception of tolerance. Justification: Dependence on other substances 
(drugs or alcohol) may result in unique CNS deficits that could confound results and introduce excessive 
variance. Assessment tool(s): The computerized SCID and clinical substance abuse/dependence 
assessment. While recreational/intermittent use of alcohol and/or marijuana will be tolerated in all 
participant groups, individuals will be excluded if they meet current or recent (within 5 years) DSM-IV 
diagnostic criteria for dependence on any substances.  A positive drug test for marijuana will not be 
exclusionary as long as participants have not used in the 24hrs preceding the imaging visits. In the event 
of a positive drug test for marijuana, self-reports of current marijuana use will be used to differentiate 
intermittent/infrequent from chronic/frequent users.  Given a possible link between marijuana use and 
psychosis (Ben Amar and Potvin, 2007) frequent users, defined as those using twice or more per week 
within the last month, will be excluded from participation.   
(4) Be able to abstain from alcohol 24hrs before each of the imaging sessions and able to moderate their 
caffeine intake 12hrs before each session.  Justification: Alcohol and caffeine modulate neural 
functioning in a way that would complicate data interpretation. Assessment tool(s): Self-report and 
breathalyzer. 
(5) Must have a urine cotinine level corresponding to smoker status for the specific test being used, typically 
corresponding to a urine cotinine above about 200 ng/ml, and have been smoking consistently for at least 
one year. For lighter smokers (less than 10 cpd), this is defined as smoking at their current level or more 
for at least the past year (excluding any quit attempts in the last year). For heavier smokers (more than 10 
cpd), they must have been smoking at least an average of 10 cpd for at least the past year (excluding quit 
attempts). Quit attempts will be assessed via clinical interview and judgment.  Smokers vary in their 
smoking topography (puff volume, time held, number of puffs etc.,) (Patterson et al., 2003) a factor which 
can significantly alter the amount of nicotine consumed from a single cigarette (Patterson et al., 2003). 
Cotinine levels should therefore provide a more accurate assessment of actual nicotine exposure than self-
reported cigarette use (Perezstable et al., 1995).. Justification: The present protocol is interested in 
neurobiological mechanisms that underlie nicotine withdrawal, and is thus contingent on the presence of 
nicotine dependence. Assessment tool(s): Self-report, commercial urine cotinine test corresponding to 
smoker status for the specific test being used, typically corresponding to a urine cotinine above about 200 
ng/ml . 
(6) Be able to abstain from smoking for 36hrs on two occasions during the study.  Justification: The present 
protocol will investigate the effect of acute nicotine withdrawal on affective, inhibitory control and 
decision making processes and their neurobiological correlates. Previous research suggests that a 36hrs 
deprivation period is appropriate to produce robust effects across these domains (Kozink et al., 2010a; 
McClernon et al., 2009b; VanderVeen, Cohen, Cukrowicz, & Trotter, 2008). To isolate effects to 
nicotine-withdrawal (as opposed to tobacco withdrawal), participants will be required to abstain from 
smoking prior to both scanning session. They will be delivered nicotine during one abstinence period 
(non-deprived) via a dose-matched transdermal patch, and will wear a placebo patch during the other 
abstinence period (nicotine-deprived).  Assessment tool(s): video recorded measurement of expired CO 
levels plasma nicotine levels. 

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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(7) Agree to also participate in NIDA-IRP protocol 10-DA-N457. 
 
Treatment-seeking smokers will also have to meet the following inclusion criteria: 
(8) Be actively seeking treatment for smoking cessation and willing to engage in 12-weeks of treatment 
involving daily administration of Varenicline and weekly counseling sessions, as well as follow-up 
assessments at 1, 6 and 12 months following treatment onset. 
 
3.3. Exclusion criteria 
All participants will be excluded if they:  
(1) are not suitable to undergo an fMRI experiment due to certain implanted devices (cardiac pacemaker or 
neurostimulator, some artificial joints, metal pins, surgical clips or other implanted metal parts), body 
morphology, or claustrophobia. Justification: MR scanning is one of the primary measurement tools used 
in the protocol. Assessment tool(s): Prospective participants will fill out an MRI screening questionnaire 
and undergo an interview with an MR technologist.  Questions concerning suitability for scanning will 
be referred to the MR Medical Safety Officer. Prospective participants will be questioned about 
symptoms of claustrophobia and placed in the mock scanner during their first visit to assess for possible 
difficulty tolerating the confinement of the scanner and for ability to fit into the scanner. 
(2) have coagulopathies, history of, current superficial, or deep vein thrombosis, musculoskeletal 
abnormalities restricting an individual’s ability to lie flat for extended periods of time. Justification: MR 
scanning sessions require participants to lie flat on their backs and remain perfectly still for approximately 
two hours. Therefore, conditions that would make that difficult (e.g. chronic back pain, significant 
scoliosis) or dangerous (e.g. familial hypercoagulability syndrome, history of thrombosis) will be 
exclusionary. Assessment tool(s): History and physical examination by a qualified IRP clinician, 
supplemented with a trial of lying in the mock scanner to assess comfort issues. 
(3) have HIV or Syphilis. Justification: HIV and Syphilis both can have central nervous system (CNS) 
sequelae, thus introducing unnecessary variability into the data. Assessment tool(s): Oral HIV followed 
by blood test if oral test is + and STS+ without adequate prior treatment 
(4) regularly use any prescription (e.g., antidepressants, benzodiazepines, antipsychotics, anticonvulsants, 
barbiturates), over-the-counter (e.g., cold medicine) or herbal medication (e.g., Kava, Gingko biloba, St. 
John’s wort) that may alter CNS function, cardiovascular function, or neuronal-vascular coupling. 
Justification: The use of these substances may alter the fMRI signal and/or neural functions of interest in 
the current study. Assessment tool(s): History and comprehensive urine drug screening to detect 
antidepressants, benzodiazepines, antipsychotics, anticonvulsants, and barbiturates. 
(5) have any current neurological illnesses including, but not limited to, seizure disorders, frequent migraines 
or on prophylaxis, multiple sclerosis, movement disorders, history of significant head trauma, or CNS 
tumor. Justification: Neurological diseases alter CNS function and, possibly, the neuronal-vascular 
coupling that forms the basis of the fMRI signal.  Assessment tool(s):  History and physical examination 
by a qualified IRP clinician, urine drug screening for anticonvulsants not disclosed by history. History of 
head trauma with loss of consciousness of more than 30 minutes or with post-concussive sequelae lasting 

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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more than two days, regardless of loss of consciousness, will be exclusionary. The MAI who will also 
retain discretion to exclude based on a history of neurological illness that may compromise data integrity.  
(6) Have any current major psychiatric disorders to include, but not limited to, mood, anxiety, psychotic 
disorders, or substance-induced psychiatric disorders, or any current suicidal ideations or history of 
suicide attempts or currently under antidepressant or antipsychotic medication treatment. The MAI will 
reserve the right to exclude on the basis of psychiatric history not explicitly described in this criterion 
Justification: Psychiatric disorders involve the central neural system (CNS) and, therefore, can be 
expected to alter the fMRI measures being used in this study. A recent FDA communication (2/1/2008) 
indicates that there may be an association between varenicline and the worsening of current psychiatric 
illness even if the illness is currently under control.  Assessment tool(s): Computerized SCID, Beck 
Depression Inventory, Beck Anxiety Inventory, Adult ADHD Self-Report Scales and clinical interview 
confirmation by clinician.  
(7) Are cognitively impaired or learning disabled. Justification: Cognitive impairment and learning 
disabilities may be associated with altered brain functioning in regions recruited during laboratory task 
performance. Cognitive impairment may affect one’s ability to give informed consent. Assessment 
tool(s): History of placement in special-education classes as a consequence of serious learning problems 
and not solely as a consequence of behavioral problems, assessed during the History and Physical 
screening assessment. 
(8) have significant cardiovascular or cerebrovascular conditions. Justification: Such conditions may alter 
blood flow, the fMRI signal and other autonomic signals, and increase risks associated with nicotine 
patch use. Assessment tool(s): History and physical exam, including 12-lead EKG. 
(9) have any other major medical condition that in the view of the investigators would compromise the safety 
of an individual during participation.  Justification: Many illness not explicitly covered here may increase 
risk or alter important outcome measures. Assessment tool(s): History and physical examination by a 
qualified IRP clinician and CBC, urinalysis, NIDA chemistry panel (liver function tests, electrolytes, 
kidney function). The following lab values will result in exclusion from the study: 
i. Hemoglobin < 10 g/dl 
ii. White Blood Cell Count < 2400/µl  
iii. Liver Function Tests > 3X normal 
iv. Serum glucose > 200 mg/dl 
v. Urine protein > 2+ 
vi. Serum creatinine > 2 mg/dl  
vii. Estimated glomerular filtration rate < 60 ml/min 
a. The MAI will retain discretion to exclude based on less extreme lab results.  After the screening 
process has been completed, the MAI will take into account all data collected in order to decide 
if there is an existing medical illness that would compromise participation in this research.  
 
(10) are pregnant, planning to become pregnant, or breastfeeding.  Females are instructed in the consent to 
use effective forms of birth control during the study period.  Justification: study procedures and drugs 

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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used in the current protocol may complicate pregnancy or be transferred to nursing children.  Assessment 
tool(s): Urine and/or serum pregnancy tests, and clinical interview.  Urine pregnancy tests will be 
conducted at the beginning of each imaging visit. 
(11) are non-English speaking. Justification: To include non-English speakers, we would have to translate 
the consent and other study documents and hire and train bilingual staff, which would require resources 
that we do not have and could not justify given the small sample size for each experiment. Additionally, 
the data integrity of some of the cognitive tasks and standardized questionnaires used in this study 
would be compromised as they have only been validated in English. Most importantly, ongoing 
communication regarding safety procedures is necessary when participants are undergoing MRI 
procedures. The inability to effectively communicate MRI safety procedures in a language other than 
English could compromise the safety of non-English speaking participants. Assessment tool(s): self-
report. 
 
 
Treatment-seeking smokers will also be excluded if they: 
(12) have moderate to severe renal impairment.  Justification. Given that renal secretion is varenicline’s 
major route of clearance, kidney impairment may result in higher systemic levels of the drug than 
intended.  Per Pfizer’s chantix insert, varenicline pharmacokinetics were unchanged in subjects with mild 
renal impairment in comparison to those with normal renal function, whereas individuals with moderate 
and severe impairment presented with varenicline levels 1.5 and 2.1-fold higher, respectively.  
Assessment tool(s): Estimated glomerular filtration rate. Renal insufficiency with estimated creatinine 
clearance < 60 ml/min calculated by the Cockcroft-Gault equation will be excluded. 
(13) are diabetic.  Justification. A recent case report describes multiple episodes of severe hypoglycemia 
experienced by a 51 year old Type-I diabetic after beginning varenicline treatment (Kristensen et al., 
2008).  The discontinuation of varenicline resolved any further hypoglycemic episodes.  The safety of 
varenicline has not been investigated in patients with diabetes.  Assessment tool(s): Casual plasma glucose 
testing.  Individuals with glucose levels above 200 mg/dl may be further evaluated for diabetes using a 
fasting glucose test or be excluded. 
4. Study Design and Methods: 
4.1. Study overview 
Treatment seeking smokers will be asked to complete three study phases: pre-treatment study visits, 
treatment visits and post-treatment follow-up visits.  
Non-treatment seeking smokers will only be asked to complete Phase 1: Pre-treatment study visits. 
 
PHASE 1 - Pre-treatment study visits 
 
Phase 1 will typically require three outpatient visits to NIDA-IRP (one orientation visit, two fMRI 
imaging visits). Visits will take place over approximately a 3-5 week period. If an individual participant’s 
schedule makes it difficult for them to complete the visits as they are described here, we will try to accommodate 

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smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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their needs when this is possible and does not interfere with the study design or study aims. For example, 
orientation training day procedures could be completed over 2 or more days. 
The orientation visit will take approximately 6 hours and each scanning visit, approximately 9 hours. 
After screening and admission into the study, participants will be scheduled for an orientation visit. During this 
visit they will complete the informed consent process as well as task training for the scanning sessions. All 
participants will complete two scanning sessions: one whilst nicotine-deprived, and another non-deprived session. 
Nicotine deprivation will be manipulated by asking participants to either wear a dose-matched nicotine (non-
deprived) or placebo (nicotine-deprived) patch during a 36 hour smoking abstinence period completed prior to 
both scans.  The order of nicotine-deprived and non-deprived sessions will be counterbalanced across participants. 
Participants will be blinded as to which sessions/abstinence periods involve nicotine- or placebo-patches. The 
double-blind with respect to the nicotine/placebo patch condition will be maintained by Dr. Betty Jo Salmeron or 
the protocol MAI. The investigator/s interacting with the participants will be blind to the nicotine/placebo patch 
condition the participant is undergoing, at the time of their interactions. “Investigator/s interacting with 
participants” includes the post-doc or research assistant who runs the study session and gathers research data. It 
does not include medical personnel such as nurses who may interact with the participant during their study visit 
but do not gather research data. 
Participants will generally receive their scan 1 nicotine and placebo patches during the orientation session, 
as well as a CO monitor, video camera and questionnaires. The patches and equipment for scan 2 will generally 
be given to participants during their scan 1 session. During both 36hr abstinence periods participants will use a 
video camera to  record themselves taking their CO levels and will report their craving and affective symptoms 
on the paper copies of take-home questionnaires. Training to measure and video record the measurement of CO 
levels will take place during the orientation session. Follow-up calls will be made to each participant as a reminder 
to commence their abstinence period.  
During each scanning visit participants will complete a series of imaging measures and several tasks to 
be complete outside of the scanner.  In addition to imaging measures and bench tasks, participants will also be 
asked to complete several psychological questionnaires (see detailed list below) and will also provide blood 
samples for cortisol and ACTH.   
 
Nicotine administration during abstinence and scanning sessions. Two essential pharmacological issues 
that are of importance in the current design relate to participant’s recent exposure to nicotine and to the selected 
dose administered to each participant. Doses will be individually tailored based on the average number of 
cigarettes smoked per day to better match daily nicotine intake (please see “Individually Tailored Nicotine Dose” 
in Section 1.7 above for justification). The dose for smokers consuming less than 10 cigarettes per day will be 
14mg to start, If they are still experiencing cravings their dose will be increased to 21mg. The dose for smokers 
consuming 10-15 cigarettes per day will be a 21mg patch and this will be increased by 7mg for each additional 5 
cigarettes/day (i.e., 21mg for individuals who smoked 10-15 cigarettes/day; 28mg for 15-20 cigarettes; 35mg for 
20-25 cigarettes, and 42mg for more than 25 cigarettes).  Nicotine patch doses up to 63mg/d have been shown to 
be safe and generally well-tolerated by smokers (Ebbert et al., 2007; Zevin, Jacob, & Benowitz, 1998). 
An IND has not been obtained for the use of the nicotine patch in this study. This study meets 
the criteria for exemption for an IND as this investigation is not intended to support a new indication 
for use or any other significant change to the labeling; the nicotine patch is already approved and 
marketed and the investigation is not intended to support a significant change in advertising; and the 
investigation does not involve a route of administration or dosage level in use in a patient population 
or other factor that significantly increases the risks associated with the use of the drug product. 
Individually tailored nicotine patch doses have been used in smoking cessation trials (Hurt, 2003) and higher 

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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doses have been shown to produce a greater reduction of withdrawal symptoms in heavier users (Ebbert et al., 
2007) .  As stated above, nicotine patch doses up to 63mg/d have been shown to be safe and generally well-
tolerated by smokers (Ebbert et al., 2007; Zevin, Jacob, & Benowitz, 1998).  
 
PHASE 2 - Treatment visits 
 
Phase 2 will involve 15-18 visits over a period of about 16-19 weeks. Within approximately 2 weeks of 
their second scan visit, participants will be scheduled for their first of up to 3 pre-treatment preparation visits. 
The number of preparation visits (1 to 3) will be determined by the study therapist on the basis of the 
participants assessed level of preparation and motivation to quit smoking. During their final pretreatment 
preparation visit, participants will work with the therapist to develop a treatment plan and set a quit date. They 
will also receive their first 2-weeks supply of varenicline during this visit (or their first treatment visit) and will 
meet with the MAI or the designated NIDA-IRP physician in the MAIs absence who will describe the 
medication, prescribed dosing schedule and potential side-effects. Participants will be instructed to take their 
first dose of varenicline 1 week prior to their quit date and will receive a phone call reminder on this day from a 
study staff member if they are not coming in for a treatment session on this day. From this date participants will 
complete 12-weeks of daily varenicline administration as well as weekly and biweekly 30min counseling 
sessions. Participants will also complete a 15 min nurse assessment during each weekly visit.  
 
PHASE 3 – Follow-up visits 
 
Participants will be asked to complete follow up visits at 1, 6, and 12 months after their last treatment visit. 
During these visits participants will complete a nurse assessment, meet with an investigator to assess quit status 
and smoking abstinence since last visit, complete a series of state measures and complete a 15-20 min scan 
session. 
 
4.2. Recruitment 
Participants will be recruited from the general population through advertising in city, regional or campus 
media, such as internet, newspapers, flyers, via referrals, or radio and television advertisements (see Appendix 
14). Participants will also be recruited from among past candidates for NIDA-IRP studies if written consent to be 
re-contacted has been obtained. We anticipate an average accrual rate of 1-2 participants per week.  
Recruitment Plan 
Individuals will be recruited for this protocol through IRB-approved advertisements and outreach 
initiatives, by referral from healthcare workers or facilities, or by referral from other study participants.  In order 
to more efficiently identify and screen volunteers for research participation, NIDA-IRP, through their recruitment 
contractors, regularly sponsor media advertisements.  Radio, television, internet and print media, as well as 
circulars, brochures, and flyers, are routinely employed in accordance with NIDA IRP clinical policy. In addition 
to media advertising the recruitment contractor may also directly contact community providers and businesses to 
increase awareness of research study participation opportunities. The Recruitment contractor may alter the size 
and/or color of these ads.  Color changes will not change the emphasis of the ad in a way that might be 
impermissible.   

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Community providers of care and services in Baltimore, Washington DC and the surrounding areas may 
be contacted for recruitment.  IRB approved materials will be offered to providers such as medical care providers 
(e.g. health clinics, urgent care clinics, individual practitioners, hospital ED’s, etc.), county or city service 
agencies (e.g. social services, health departments, human resources, adult education centers, etc.), and community 
service providers (e.g. shelters, Goodwill Industries, employment centers, crisis centers, etc.). A list of providers 
contacted will be submitted to the IRB at the time of each Continuing Review. 
 
NIDA Staff or contractors may also go to community businesses such as libraries, community centers, 
convenience stores, grocery stores, food venders, and other local businesses, etc. to request permission to post 
IRB approved recruitment materials.  Posting approval will be consistent with the venue such that the customary 
approval process for posting will be followed and documented. 
 
In addition, staff from the NIDA IRP and NIDA IRP recruitment contractor may go on site to community 
locations to recruit participants.  When NIDA outreach staff is on site, they may post signs in the lobby or waiting 
area advertising that NIDA will be recruiting for one of its studies.  Also, an announcement may be made that 
NIDA is on-site that day to recruit for one of its studies and that anyone interested in more information can go to 
the designated area to learn more.  Interested people will meet one-on-one at the site with an outreach staff person 
who will answer specific questions about the protocol. All advertisements will be submitted for approval by the 
IRB before being used.  Written permission by the director from each site will be obtained prior to recruiting.  This 
permission will include a statement about their approval of our presence in their site to recruit for studies, plus, 
where applicable, an assurance that a person’s choice to be involved in this screening or study will not in any way 
affect their participation there. 
 
Outreach Events: 
 
B’More Healthy Expo 
      Saturday, February 28, 2015 (10A-5P) 
      Baltimore Convention Center 
      30,000 + attendees 
      20x20 booth  
Email advertisement - WBFF will send out our currently approved email, twice, to up to 50,000 people 
who agreed to be emailed sponsor information. The email used will the exact same one approved by the 
IRB under protocol 474 amendment 12(L), which is reattached with this PTMS submission or reference. 
 
NIDA-IRP personnel would be available to meet potential candidates interested in NRB smoking 
cessation studies to tell them about our active studies and to answer any questions they may have. We would hand 
out IRB approved materials such as fliers, business cards and study fact sheets. Our booth would only be decorated 
with IRB approved materials.  
 
We will take NIH-issued, password-protected laptops so that interested people can leave their information 
with us. We have worked with Massoud Vahabzadeh, Chief of the Biomedical Informatics Section (BIS), to create 
a system to protect the privacy of individuals interested in participating in studies. BIS will create a database to 
record candidate information directly so that they cannot see other people’s information. He also suggested that 
as a back-up, (e.g. power failure) we should take index cards so that we can have people write down their 

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smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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information for us. Again, this method would eliminate the possibility that they would see anyone else’s 
information as we would give each person their own card. 
 
These are the fields BIS will use to create the database, some with dropdown choices: 
 
First Name 
Last Name 
Primary Phone# 
Alternative Phone# 
Email address 
Best contact method: (email, phone) 
Preferred contact time: (anytime, morning, afternoon, evenings) 
Smoking Status: (non-smoker, smoker interested in treatment, smoker not looking for treatment, ex-
smoker) 
 
Participant Testimonials: 
We will develop ads using real participant testimonials with a photo and a quote from participants who 
are willing to have their photos and quotes used as ads. Participant testimonials are currently used at NIH 
(https://www.drugabuse.gov/related-topics/treatment/one-patients-story-nida-clinical-trials-bring-new-
life-to-woman-struggling-opioid-addiction  and https://www.nih.gov/health-information/nih-clinical-
research-trials-you/personal-stories). We are interested in using high-impact quotes from similar 
interviews with our current and former participants (both treatment seeking and non-treatment) to 
improve the authenticity and relatability of our advertising campaigns. 
 
We would ask participants if 1) they would like to tell us about their experience in this study and have us 
use their picture and quote used for advertising purposes. If they are interested, we may ask them 
questions such as those listed below, then develop the ads, submit to PILB and then to IRB. The ads 
would look very similar to current ads with a quote from the participant.  These ads would be posted as 
currently approved ads do, in print, online, social media, and on the NIDA website. Past subjects may 
also be contacted via phone so that we may ask them if they are interested in doing a testimonial ad with 
NIDA. Participants will be notified that not all quotes will be used and that the final ad may not be 
published. They will also be asked to sign a media release form if they would like to have their photo 
and/or quote used in an ad. 
 
“What would you say to someone who is thinking about being in this study?” 
“How has being in this study affected your daily life?”  
“When considering personal interactions throughout the duration of the trial, how would you describe 
your experience?” 
4.3. Screening methods  
Candidate participants will be screened for participation based on the Inclusion criteria/Exclusion criteria 
assessment tools listed above, under the aegis of the IRB approved NIDA-IRP screening protocol, 06-DA-N415. 
Informed consent will be obtained from prospective participants prior to the commencement of any screening 
procedures in a private area where participants are free to ask questions.  During screening participants will be 
given some initial information regarding the study (see Appendix 2 “Fact Sheet”) to determine if they are 
interested in possible participation.  

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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The assessments to be done during screening include the following: 
1. Beck Anxiety Inventory (BAI; Beck, 1993) - A 21-item self-report inventory, which assesses the severity of 
anxiety.  The BAI has demonstrated high internal consistency with an alpha reliability of .92, and high test-
retest reliability demonstrated by a correlation of .75 between tests taken one week apart.  In addition, the 
BAI has shown strong convergent validity with BAI scores correlating significantly with other clinical 
measures of anxiety (correlations range from .25 to .51), and strong discriminant validity with patients 
diagnosed with an anxiety disorder scoring significantly higher on the BAI than depressed patients and 
controls (Beck, 1993). This questionnaire is estimated to take less than 5 minutes to complete. 
2. Beck Depression Inventory (BDI; Beck, 1996) - The BDI-II is 21-item self-report inventory that assesses 
symptoms of depression currently and in the preceding seven days. Each item is rated on a 0-3 scale with 
summary scores ranging between 0 and 63. Studies have shown the BDI-II to have high test-retest reliability 
(r=.93, p<.001), high convergent validity in that it correlates more positively with other measures of 
depression (r=.71) than measures of anxiety (r=.47), and no association with demographic variables such 
as gender, ethnicity, and age (Beck, Steer, Ball, & Ranieri, 1996).  These findings suggest that the BDI-II 
is a valid and reliable assessment tool regardless of population. This questionnaire is estimated to take less 
than 5 minutes to complete.   
3. Adult ADHD Self-Report Scale (ASRS v1.1; Harvard School of Medicine, 2005) – This questionnaire is an 
18-item checklist designed by the World Health Organization (WHO) and Workgroup on Adult ADHD to 
determine whether a participant is suffering from symptoms related to attention-deficit/hyperactivity 
disorder. It requires about 5 minutes to complete. 
4. Edinburgh Handedness Inventory (Oldfield, 1971) – This 12-item questionnaire assists in determining the 
dominant hand (or handedness) of a particular participant by inquiring which hand the participant uses for 
various tasks.  This task requires an estimated 2-5 minutes to complete   
5. Fagerstrom Test for Nicotine Dependence (FTND; Heatherton et al., 2001) A 6- item test that measures the 
severity of nicotine addiction. Estimated time to complete is 1-5 minutes. 
6. Brain Imaging Information Handout (see attached material) – A general information sheet on brain 
imaging research with common questions and answers is given to subjects as an informational item only.  
Subjects are given time to read the handout and to ask questions about it while in clinic (time to complete, 
20 minutes). 
7. Medical Assessments – Vital Signs, height/weight measurements, urinalysis, EKG, oral HIV test, blood 
draw, 
urine 
qualitative 
drug 
screen 
for 
methadone, 
benzodiazepines, 
cocaine, 
amphetamine/methamphetamine, opiates, barbiturates, tetrahydrocannabinol, and tricyclic antidepressants, 
urine toxicology which analyzes for presence of a broad range of prescription and nonprescription drugs, 
as well as urine pregnancy testing for menstruant females, and urine cotinine level testing to verify smoking 
or nonsmoking status.  Breathalyzer for alcohol and exhaled breath CO measurement is taken at this time 
as well.  Urine specimens may be collected under observed conditions if required by the specific protocol 
being screened for.  Staff member observing urine collection will be same sex as subject and will observe 
through one way mirror. Estimated time to complete is 30-40 minutes. 

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8. Contact Information Form - This form requests multiple methods of contact for a participant, as well as the 
preferred contact method, preferred contact time, and emergency contact information. Estimated time to 
complete is 2-5 minutes. 
9. Participant Safety – MRI Screening Form - A questionnaire that aids in determining whether a participant 
can safely have an MRI scan. Estimated time to complete is 3-5 minutes. 
10. Medical History and Physical Examination –This is a standard physical and detailed medical history and 
physical examination designed to capture the inclusion and criteria for NIDA-IRP protocols.  It is 
administered by a Physician’s Assistant or other credentialed medical staff. The estimated time to complete 
the H&P exam is variable; it is dependent on both the individual and the protocol(s) for which s/he is being 
considered. The examination typically lasts between 45 – 90 minutes. 
11. Mock Scanner:  Subjects who qualify for MRI scanning as determined by the interview and the MRI Safety 
form are taken to the Mock scanner during their screening visit. If the mock scanner is occupied during 
their screening visit, subjects are brought back to try the mock scanner before any actual MRI scanning 
session.  The scanner environment is explained to the participant and they are asked to lie in the scanner to 
make sure there are no problems with body fit or comfort.  This give subjects the opportunity to opt out of 
scanning experiments if they are not comfortable with the scanner set up. Estimated time to complete is 5-
10 minutes.  
12. Blood Draw – Blood is taken from a vein in the hand or arm. Tests on the sample include CBC, complete 
metabolic profile, TSH, ESR, STS, Hepatitis B surface antigen, Hepatitis C antibody and HIV (if needed 
to confirm a positive salivary test for HIV), Prothrombin time (PT), Parial Thromboplastin Time (PTT), 
and Creatine Kinase (CPK). Estimated time to complete is 20-30 minutes. 
13. SCID Screen Patient Questionnaire – Extended (SSPQ-X) (1) This test is the computer-administered 
screening version of the Structured Clinical Interview for DSM-IV™ (SCID).  While there are a total of 
589 questions in this program, participants are not presented with all questions. Questions that are presented 
are dependent upon the participant’s answer to the previous question. The disorders covered include Mood 
Disorders, Anxiety Disorders, Substance Use Disorders, Psychotic Symptoms, Somatoform Disorders, and 
Eating Disorders.  SSPQ-X also differentiates between Substance Dependence and Substance Abuse and 
includes questions for each type of drug endorsed. Estimated time to complete is 25 to 45 minutes. 
14.  Drug Use Survey (DUS) An interviewer-administered questionnaire designed to collect information 
regarding lifetime history of substance use.  It requires 10-40 minutes to complete, depending on history of 
use. 
15. Toronto Alexithymia Scale (TAS-20) (6) A twenty item scale that measures difficulty with awareness of 
feelings.  Estimated time to complete is 2-5 minutes.  
 
4.4. Study procedures 
(i) Orientation and task training 
Before commencing the scanning sessions, participants will complete a training session where they will 
be instructed on and practice the experimental tasks in the mock scanner and be trained for abstinence period 
procedures. Participants will not be able to smoke during the orientation visit. 
 

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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1) Nurse Assessment. After completing the consent interview, participants will undergo a medical update, 
which will include vital sign assessment, ECG (during orientation), MRI compatible ECG electrode placement 
(scan sessions), a breathalyzer test for expired ethanol, an expired CO test for smoking-abstinence compliance, a 
urine test for common abused drugs, a metal/MRI safety screening on scan days, and if female, a urine pregnancy 
test. In the event of a positive drug test for marijuana, an interview to assess last drug use and a Neuromotor Drug 
Influence Evaluation (Dr. Steve Heishman, personal communication) to test for acute intoxication will be 
performed. The purpose of the nurse assessment is to provide additional screening for characteristics (e.g., 
pregnancy, positive drug tests, metal in body) which would exclude an individual from participation in the study 
on that day. If participants test positive for drugs other than marijuana they will be rescheduled, however, if they 
test positive on a second occasion they will be excluded from the study. This will be made clear to the participant 
in the consent process. In addition, participants will be asked to report time of last meal, hours slept the previous 
night, recent medication use, changes is medical history since last visit and, if female, last menstrual period and 
whether or not they are currently taking a contraceptive pill. 
 Information obtained during the nurse assessment is stored confidentially and securely in the individual’s 
medical file (See section 5.0 – Storage of Data and Samples). ~30min 
 
2) Baseline and characterization questionnaires. A series of questionnaires (see Appendix 3) will also be 
administered during the orientation visit. ~1hr 
 
4) Nicotine patch toleration test. Participants will complete a nicotine patch toleration test during the 
orientation visit. Nursing staff will apply the dose-matched nicotine patch at the end of the nurse assessment and 
will describe potential side effects to the participant. Participants will wear the patch for approximately 3 hours 
and will be asked to notify the LI or nursing staff immediately if they experience any worrisome side-effects. A 
nurse will remove the patch and conduct a post patch assessment including vital signs, neck/back pain assessment, 
mental status exam, and skin assessment.  
 
3) Mock scanner training session. The mock scanner training session will familiarize participants with 
study procedures and the scanner environment.  Given that training and scanning will take place on separate days, 
individuals will receive training refreshers during subsequent visits. ~ 1.5hrs 
 
5) Abstinence period procedure training. Participants will also be trained to measure their CO levels and 
record this using a small video camera. (see Abstinence Periods section for details). At the end of this visit they 
will generally also receive their first set of nicotine or placebo patches and will be instructed on the application 
of these during the abstinence periods as well as the time points and procedure for recording their CO and 
mood/withdrawal symptoms during their upcoming abstinence period. Subjects will also be provided with written 
instructions on how to apply each patch (see Appendix 5).  CO monitors and video cameras and paper copies of 
self-report measures to complete during the abstinence period will also be distributed to participants at the end of 
their orientation session. Participants will also be provided instruction in cognitive behavioral techniques (e.g., 
progressive relaxation) designed to reduce withdrawal discomfort and improve compliance to the 36 hour 
abstinence procedure ~40min 
 
6) Assessment of motivation and preparation to quit. Several measures assessing participant motivation 
and preparation to quit smoking (see Appendix 9) will be administered to the treatment-seeking smokers and 
those who enroll in the motivational arm. ~20min  

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 (ii) Abstinence periods 
During both the nicotine-deprived and non-deprived abstinence period participants will use a small video 
camera to video record themselves measuring their CO levels. They will also report mood and withdrawal 
symptoms at 6 time points throughout each abstinence period: 10pm on day 1 (2hrs post-cessation); 8am on day 
2 (12 hrs post-cessation); 12noon on day 2 (16 hrs post-cessation); 4pm on day 2 (20hrs post-cessation); 8pm on 
day 2 (24 hours post-cessation); 10pm on day 2 (26 hrs post-cessation). All times are approximate. Their CO 
levels will be measured again at 8am on day 3 (36 hrs post-abstinence) when they arrive at NIDA-IRP for their 
scanning session. Research staff will generally phone participants on the afternoon they are to commence their 
abstinence period. Participants may also receive text message reminders from research staff at each of the six 
measurement time-points depending on participant needs. This procedure will allow the research team to monitor 
participant CO levels throughout the 36 hr abstinence periods, validating smoking abstinence. It will also provide 
a method of measuring changes in mood and withdrawal symptoms throughout the 36 hr abstinence periods across 
both deprived and non-deprived conditions. This information will provide a measurement of responsivity to 
nicotine replacement. 
(iii) Scanning Visits.   
There will be two scanning visits, both preceded by a 36 hr abstinence period. During one abstinence 
period and scanning session participants will wear a dose-matched nicotine patch. A placebo patch will be applied 
throughout the nicotine-deprived abstinence period and scanning session. The order of deprived and non-deprived 
sessions will be counterbalanced across subjects. Scanning sessions will be separated by a minimum of 5 days. 
The two scanning visits will be essentially identical. Each visit will consist of two 1.5 to 2 hr scanning sessions, 
one taking place in the morning (AM) and one in the afternoon (PM).  Participants will also complete several pre- 
and post-scan tasks and a series of questionnaires throughout the day. All imaging visits will take approximately 
9hrs (8am to 5pm) to complete. The order of procedures completed during each scan visit is outlined schematically 
in Figure 2, and described in detail below. 
 
 
 
Figure 2. A schematic outline of the procedures completed during both deprived and non-deprived 
scanning sessions (note: all times approximate and order of tasks and scans generally fixed but may be switched 
in the event of time constraints, instrument problems or subject compliance). 
 
1) Nurse Assessment. Upon arrival at NIDA-IRP participants will return their video camera and CO 
monitor to research staff. They will then undergo a scan day nurse assessment. See orientation day Nurse 
Assessment for procedure details. 
 

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2) Pre-scan task practice (AM). Prior to scanner prep, participants will be reacquainted with the task 
instructions and will complete brief training runs.  The task instruction/practice period will last ~30 min.  
 
 
3) EEG Preparation. Simultaneous EEG and fMRI data will be collected during all scanning sessions.  
Therefore, an EEG preparation period lasting approximately 45min will always precede the morning scanning 
session.  
 
Participants will be asked to wash their hair the night before/morning of the scan, or alternatively, upon 
arrival at NIDA-IRP. They will also be asked not to wear any hair product on the day of the scan. Prior to cap 
placement they will be given a comb and asked to brush their hair thoroughly.  The investigator will then place 
the fMRI compatible EEG cap onto the participant’s head and then adjust the cap position so that electrode 
locations correspond to conventional recording locations (10/20 system).  In addition, an electrode will be placed 
on the upper back to monitor cardiac activity.  The experimenter will then insert a conductive gel into each of the 
electrodes so that good electrical contact can be made between the scalp and the electrodes.  Electrode impedances 
will be reduced below 10kΩ during preparation to ensure quality EEG recording.  The location of each of the 
electrodes will be digitally recorded and used to model the electrical generators of EEG activity in subsequent 
analyses. 
 
4) Self-report measures. Participants will be administered several brief measures to assess current anxiety, 
arousal, mood, and other withdrawal symptoms. These measures will be administered on a computer in a private 
area and include those indicated in Appendix 4.  
 
5) Scanner Preparation. Approximately 15min is needed to position the participant in the scanner bed, 
setup subject response pads, and prepare other equipment for the recording session.  
 
All scanning experiments will be performed on a research-dedicated 3T MRI scanner, located at the 
NIDA-IRP.  The 3T MRI machine used in this protocol is FDA approved and is used as a data collection tool. 
This study may use the MRI in research mode, including custom pulse sequences. All sequences, including those 
in research mode, are within FDA approved specific absorption radiation (SAR) limits. No additional risk is 
incurred in research mode, and we are not testing sequences to evaluate safety or risk. 
Participants’ heads will be placed inside the RF coil and held in place by a head restraint that may consist 
of soft foam, a vacuum bag, a bite bar and/or hardened polyurethane foam (expanding packing foam).  For most 
scanning experiments, a blipped, gradient-echo, echo-planar image (EPI) pulse sequence (e.g. TE = 27ms) is used 
with a TR of about 2 seconds.  Images will be acquired with an in-plane resolution of 64 x 64 and with a 22cm 
field of view.  Specific imaging parameters (e.g. number of slices, slice angle, TR, TE, flip angle etc.) may be 
changed in order to maximize signal integrity.  Please note, that we use a “watchdog” program that runs 
continuously on the MRI computer during the execution of all pulse sequences and ensures that no scanning 
parameter ever exceeds FDA guidelines. The MRI is used as a data collection tool. All sequences and scans 
conducted are within FDA approved parameters, and the scans are not conducted to assess safety or efficacy of 
the scans themselves. 
 
6) AM Scan Session (note: all times approximate and order of scans generally fixed but may be switched 
in the event of instrument problems or subject compliance). 
 

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Structural Images. A set of high resolution structural MR images will be collected. The images will be 
acquired using a T1-weighed three-dimensional MPRAGE sequence and will be used for the display of functional 
data.  Completion time: ~5 min. 
Affective Multi-source Interference Task (affective MSIT). Participants will complete a modified Multi-
source Interference Task designed to study the ERP and fMRI correlates of performance monitoring, conflict 
resolution and post-error adjustment processes within an affective context. The MSIT combines several sources 
of combines aspects of the simon, stroop and flanker tasks to produce a strong and reliable conflict manipulation 
(Bush, Shin, Holmes, Rosen, & Vogt, 2003; Bush & Shin, 2006). Each trial involves the presentation of three 
digits which will include 0s, 1s, 2s or 3s. One of the three digits will be different from the other two (the oddball). 
Participants indicate the identity of the oddball (which will always be a 1, 2 or 3) by pressing one of the three 
buttons. On control trials (no conflict) the oddball digit is in the same spatial location as the response button and 
the other two digits are zeros (e.g., 100 or 020). On conflict trials the oddball is in a different location to the 
response button and the other two digits are competing response choices (e.g., 211, or 232). We have included an 
additional affective component to the standard MSIT, by manipulating the consequences of correct (win 0, 5 or 
50 points) and slow/incorrect responses (lose 0, 10 or 100 points). To increase the likelihood of errors, participants 
are only awarded points for correct responses if they exceed an individually determined time threshold. 
Participants will be able to “cash in” points at the end of task and earn up to $50 depending on the number of 
points accumulated. Completion time ~40min 
Diffusion tensor imaging (DTI) scan. A DTI scan will be performed for the assessment of white matter 
microstructure. Previous research has suggested that some addiction-related deficits in cognitive task performance 
can be related to impaired anatomical connectivity between brain regions (Pfefferbaum et al., 2000; Lim et al., 
2002; Madden et al., 2004; Moeller et al., 2005). There is also evidence that the integrity of connectivity between 
medial PFC and amygdala regions may mediate trait reactivity to stress (Kim & Whalen, 2009), a central construct 
of interest in the current protocol. Thus DTI may reveal important pre-existing structural characteristics of 
relevance to individual differences in acute nicotine withdrawal. Completion time: ~5min. 
Go/No-go Task: Inhibitory control processes - cognitive context.  Participants will perform multiple 
blocks of a Go-NoGo task designed to study the ERP and fMRI correlates of response inhibition, error detection 
and post-error behavioral adjustments. The goal of the go-nogo task is to press a button following each target 
stimulus (go-trial) and to withhold a response following each lure stimulus (NoGo-trial).  In each block of the 
task participants will view a serial stream of alternating Xs and Ys.  Participants will be instructed to make a right-
handed button press response to each target stimulus (an X or Y that is different from the last X or Y stimuli 
presented) while the stimulus is still on the screen.  In contrast, responses will need to be inhibited following lure 
stimuli, defined as a non-alternating target stimulus (i.e., the second of two consecutively presented identical 
stimuli such as XX).  Within each block, most of the stimuli will be targets, while only a small portion will be 
lures. Task blocks will be interspersed with resting fixation blocks which will be employed as a baseline for some 
analyses. Performance on this task is quantified as the number of successful inhibitions that participants are able 
to achieve.  Stimulus duration on this task will be individually tailored to produce an equivalent number of 
successful inhibitions and commission errors across participants.  Specifically, during training in the mock 
scanner environment an appropriate difficulty-level will be selected.  The combination of shorter stimulus 
durations and the instruction to respond while the stimulus is on-screen, yields increased numbers of commission 
errors (Garavan et al., 2002).  Robust differences in brain activity and behavioral performance have been observed 
using this paradigm to study cocaine addicts (Kaufman et al., 2003). Completion time: ~20 min. 
 
 
7) Break. Following completion of the AM-session, participants will take a break. Prior to the break 
laboratory technicians will remove the EEG cap from their head and allow the participants to wash and dry their 

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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hair. During the break time participants will be able to have lunch, relax, and spend time in the day room at their 
leisure. The break will last ~60 min. 
 
8) Plasma nicotine. Immediately after lunch a nurse will fit an indwelling catheter into a vein on the 
participants arm, wrist, hand or foot. The selection of which vein to fit the indwelling catheter may vary between 
participants. Immediately after the indwelling catheter has been successfully inserted, and before the PM scan 
session, a nurse will take a blood sample from participants that will later be used to assess for plasma nicotine 
and cotinine levels.   
 
9) Bench tasks:  
Rapid Visual Information Processing Task. Attention will be assessed with the rapid visual information 
processing task (RVIP; Wesnes & Warburton, 1983). Individual differences in attention can significantly alter 
performance across a range of tasks. It is thus important to control for treatment and withdrawal-related changes 
in attention. The RVIP provides a quick and reliable measure of sustained attention. The task has participants 
monitor a continuous stream of single digits presented on a screen in front of them and respond with a button 
press when they notice three odd or three even numbers presented consecutively (e.g., 5,9,7 or 4,2,8). Completion 
time ~10 min 
Delayed Discounting. Following the break participants will complete a delay discounting task (Hariri et 
al., 2006), which assesses the discounting of incentive value with delay to receipt of reward. On each trial 
participants will be asked to select between a hypothetical amount of money received that day (e.g., $0.15 to 
$150) and a large amount (e.g., $150) received after a delay ranging from 0 days to 5 years. For each delay interval 
(e.g., 0 days, 7 days, 2 years) a ‘switch point’ is calculated as the average (in dollars) of the last immediate value 
that the participant selected and the next lower immediate value (i.e., the value at which participants opted for the 
delayed reward). Switch point values are plotted against delay intervals to calculate a delayed discounting curve 
– with steeper curves indicating greater discounting of incentive value with delay to receipt of reward.   
Completion time: ~10-20min. 
Probabilistic reward task.  The probabilistic reward task was designed to produce a behavioral index of 
reward responsiveness and shows sensitivity to anhedonic symptoms. The task involves three blocks of 100 trials 
which each commence with the presentation of a simple line-drawing of mouthless face. The mouthless face is 
first presented for 500ms, followed by the presentation of the same face with either a short or long mouth 
superimposed for 100ms. Participants are required to indicate whether the mouth presented was long or short. For 
each 100 trials, only 40 correct responses were awarded with positive feedback and points (“Correct!! You won 
20pts”). For half of the participants correct responses to the short mouth will be awarded three times more than 
correct responses to the long mouth, and vice versa for the other half of the participants. The task yields two 
indices of reward responsiveness: reward bias, which is the systematic preference for the more heavily rewarded 
stimulus, and reward learning, which is the difference in reward bias from block 1 to block 3. Participants will be 
able to “cash in” points at the end of task and earn up to $10 depending on the number of points accumulated. 
Completion time ~20min. 
 
10) Pre-scan task practice (PM). As above for AM session. 
 
11) PM scan session (note: all times approximate and order of scans generally fixed but may be switched 
in the event of instrument problems or subject compliance). 
 
Structural scans. As above. ~5min 
 

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Unpredictable Shock and Cue Reactivity Task (USCRT): to assess activity in the BNST and CeA. The 
USCRT was developed to assess the effect of state activation in the BNST and CeA upon reactivity to drug-
related and neutral cues. Unpredictable shock produces a state of sustained anxiety mediated by the BNST in 
rodents (Davis et al., 2010) and shown to activate the BNST and CeA in humans (Alvarez et al., 2011).  
Smoking-related (e.g., person inhaling on cigarette) and neutral (e.g., person holding a pencil in their 
mouth) pictures will be presented under either an unpredictable shock condition (during which a shock could 
occur at any time) or a safe condition (during which participants are explicitly informed that no shock will occur). 
The shocks will be delivered through two disk electrodes placed on one of the feet or ankles, using a constant 
current AC stimulator. The shock will have intensity up to 4.0 mA and duration up to 500ms. The shock is 
generally described by participants as rather anxiogenic and unpleasant, but not painful. The mean rating of 
aversiveness on a scale of 1 (not at all painful) to 10 (extremely painful) is about 5. The procedure for 
administering the unpredictable shock is based on that described by our colleagues at NIMH (led by Christian 
Grillon) who developed the unpredictable shock paradigm. Their experience is that over 95% of participants who 
received the shock chose to participate in the experiment.  Participants will not receive the shock until the first 
scan visit for two reasons. Firstly, additional exposure to the shock is likely to produce habituation to the 
unpleasant quality of the shock and reduce the degree of anticipatory anxiety that presentation of the unpredictable 
shock stimulus elicits. Secondly, the level of shock presented to participants in the current study is determined 
via a shock work up procedure, which commences with a very low level of shock delivery which most individuals 
cannot detect. The level is then increased to a level which produces discomfort but is also tolerable for 
participants. We therefore do not anticipate any withdrawal from the study due to inability to tolerate shock.  
Once the electrodes are placed, and prior to commencing the experiment, we will perform a shock work-
up procedure during which participants receive between 1and 5 shocks of increasing intensity and select and are 
asked to rate each shock, indicate when the level of the shock is perceived as unpleasant and tolerable but not 
painful. As a consequence, no participant is presented with a shock that exceeds this perceived level of discomfort. 
Participants will have the opportunity to withdraw from the study if they wish at any moment, and are informed 
explicitly about this option in the informed consent. Self-report measures of craving, mood, arousal and distress 
will be taken throughout the procedure. Completion time ~ 25min 
 
Spectroscopy (MRS) scan: Measure of intra and extracellular glutamate.  As described above, acute 
nicotine withdrawal is associated reduced glutamatergic transmission at the VTA and PFC/ACC. A proton 
magnetic resonance spectroscopy (MRS) scan of pre-defined ROIs within these regions will be conducted to 
examine the effect of acute nicotine withdrawal on extra- and intra-cellular glutamate detected by MRS. 
Completion time: ~20 min  
 
Amygdala Reactivity Task.  Participants will complete a simple perceptual task previously shown to 
produce robust bilateral amygdala activity (Hariri et al., 2002; Pezawas et al., 2005; Foland-Ross et al., 2010), as 
well as top-down prefrontal regulation of amygdala reactivity (Hariri et al., 2002; Foland-Ross et al., 2010).  This 
blocked fMRI paradigm consists of two experimental conditions: a face matching condition, and a sensorimotor 
control condition. During the emotion matching condition, participants view an array of 3 faces and their task is 
to select (via button press) one of the two faces matches the identity of the target face. During the sensorimotor 
control task, participants view an array of 3 geometric shapes (circles, vertical and horizontal ellipses) and select 
one of the two shapes (bottom) that matches the target shape (top).  Each block begins with a brief instruction 
screen (“match face” or “match shape”). During each emotion block different images selected from a standard set 
of pictures of facial affect (Ekman and Friesen, 1976), are presented. During the control blocks, different 
geometric shapes are presented in a pseudo-random order.  Completion time: ~7min.     
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Striatal Reactivity Paradigm (SRP). The SRP provides a robust probe of ventral striatal (VS) activity 
associated with positive and negative feedback (Hariri et al., 2006).  At the start of each trial participants will be 
presented with a card displaying a question mark on the center of the screen. They will be instructed to guess 
whether the value of the card was greater than or less than 5 by pressing one of two buttons. Participants are then 
presented with the value of the card and a green up arrow (positive feedback) or red down arrow (negative 
feedback) indicating the accuracy of their guess. They are also informed that their performance will determine 
the size of a monetary reward to be received at the end of the scanning session. Unknown to the participants, the 
outcome of each trial (i.e., positive or negative feedback) is predetermined. The task will be presented in a blocked 
design with positive feedback (correct on 75% of trials) and negative feedback blocks (correct on 25% of trials) 
interspersed with control blocks during which participants are instructed to make alternating button press 
responses when they see an ‘x’ on the center of the screen. Participants can earn up to $10 on the task. Completion 
time: ~5min 
 
Resting Scan. An EPI scan to assess resting-state fluctuations (RSF) will be conducted. These scans will 
provide us with information relating to trait and perhaps state connectivity between different prefrontal and limbic 
brain regions. Completion time: ~10 min. 
 
Heart rate, heart-rate variability and respiration. Heart rate (HR) and heart-rate variability (HRV) will 
be measured throughout the two scanning sessions via MRI compatible ECG electrodes placed on the 
participant’s chest or back and a Pulse-Ox monitor attached to the individuals forefinger HR provides a 
sensitive measure of tonic and phasic changes in physiological arousal in response to errors (Hajcak et al., 
2004), drug cues and (for review see Carter & Tiffany, 1999) and threat of shock (Rhudy and Meagher, 2000; 
Bradley et al., 2008). Resting HRV provides a trait measure related to enhanced resilience to stress and affect 
regulation (Friedman and Thayer, 1998; Hansen et al., 2009). Respiration will be measured with a thoracic 
strain-gauge belt that will be fitted around the individual’s waist to control for respiration-related changes in HR 
and HRV.  HR, HRV and respiration will be measured during all task and resting functional scans.  
 
11a) Plasma cortisol and ACTH: collection and analysis. Blood samples will be taken before and during 
the PM scan session in order to measure levels of plasma cortisol and ACTH. The nurse will be present throughout 
the PM scan session to take one 7 ml sample of blood at the three different time-points illustrated in Figure 3. 
 
 
 
 

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smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Figure 3. A schematic outline of the scanning procedures and cortisol/ACTH plasma sampling time 
points during the PM scan session. 
 
12) Post Scan Nurse Assessment. A nurse will remove the IV line and patch, obtain vital signs, and complete 
a neck/back pain assessment, mental status exam, and skin assessment.  
 
13) Debriefing (end of day).  At the end of the PM scanning session on scan day 1 there will be a debriefing 
period for discussion of questions/issues related to the day’s tasks, scheduling/reminder of subsequent visit. They 
will also schedule a time close to their next abstinence period during which they will briefly visit one of the 
research staff to pick up their second set of placebo or nicotine patches, CO monitor, flip camera and forms to 
record mood/withdrawal symptoms. They may take these items home if their scan 2 is within a week or two and 
the equipment is not needed for another participant. 
 
After Scan day 2 debriefing, treatment-seeking participant’s motivation and preparation to quit will be 
reassessed and they will be scheduled for their first treatment preparation visit with the study therapist. 
(iv) Research and Clinical Care Procedures. 
Clinical care for the treatment-seeking smokers will be provided as soon as participation in the first two scan 
sessions is complete. For details of clinical care procedures please refer to Section 4.4 Study Procedures (v) 
Treatment below. All participants entering into the study will be advised during the consent process that nicotine 
patches used during the first two abstinence periods and scan visits are strictly for research purposes.  
(v)Treatment 
1) Treatment Overview: 
Within approximately 2 weeks of their second scan visit, participants will be scheduled for their first of 
up to 3 pre-treatment preparation visits. The number of preparation visits (1 to 3) will be determined by the 
study therapist on the basis of the participants assessed level of preparation and motivation to quit smoking. 
During their final pretreatment preparation visit, participants will work with the therapist to develop a treatment 
plan and set a quit date. They will also receive their first 2-weeks supply of varenicline during this visit or their 
first treatment visit. During either visit they will meet with the MAI or the designated NIDA-IRP physician in 
the MAIs absence who will describe the medication, prescribed dosing schedule and potential side-effects. 
Participants will be instructed to take their first dose of Varenicline 1 week prior to their quit date and will 
receive a phone call reminder on this day from a study staff member if they are not already here for a treatment 
session that day. From this date participants will complete 12-weeks of treatment including daily varenicline 
administration (for 12 weeks) as well as weekly and biweekly 30min counseling sessions. Participants will also 
complete a 15 min nurse assessment during each weekly visit.  
 
Week 1  
begin taking varenicline (0.5 – 1mg/day); counseling; nurse assessment; questionnaires 
Week 2 
Quit Date; varenicline (2mg/day); counseling; nurse assessment; questionnaires. 
Week 3 
varenicline (2mg/day); counseling; nurse assessment; questionnaires 
Week 4  
varenicline (2mg/day); counseling; nurse assessment; questionnaires 
Week 5  
varenicline (2mg/day); counseling; nurse assessment; questionnaires 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Week 6  
varenicline (2mg/day); counseling; nurse assessment; questionnaires 
Week 7  
varenicline (2mg/day); counseling; nurse assessment; questionnaires 
Week 8  
varenicline (2mg/day); counseling; nurse assessment; questionnaires 
Week 9 
varenicline (2mg/day); phone assessment 
Week 10 
varenicline (2mg/day); counseling; nurse assessment; questionnaires 
Week 11 
varenicline (2mg/day); phone assessment 
Week 12 
varenicline (2mg/day); counseling; nurse assessment; questionnaires 
Week 13 
phone assessment 
Week 14 
counseling; nurse assessment; questionnaires. 
Week 16 
1 month follow-up counseling; nurse assessment; questionnaires 
 
Please note that all times described in the treatment outline are approximate and if a person cannot attend a 
scheduled visit we will reschedule it for the next available date.  
 
2) Varenicline Administration. All participants will follow the dose schedule outlined below: 
 
Week 1: 
Days 1 to 3: Take 1 x 0.5mg pill each day in the morning.  
Days 4 to 7: Take 2 x 0.5mg pills each day. Take one in the morning and one in the evening.  
 
Week 2 to 12:  
Take 2 x 1mg pills each day.  Take one in the morning and one in the evening. 
 
Participants will be instructed to eat prior to dosing, to take their dose with a full glass of water, and to 
take doses at least 8hrs apart (please see Appendix 8 for take-home study pill instructions).  As noted above, 
they will receive a 2 week supply of varenicline during their initial interview. They will then receive an 
additional 1-2 week supply of varenicline on a weekly or bi-weekly basis from weeks 2 to 10.   
 
3) Pretreatment preparation visits. Participants will be scheduled for 1 to 3 pretreatment preparation visits 
with a study therapist. The number of preparation visits will be determined by the study therapist on the basis of 
the participants assessed level of preparation and motivation to quit smoking. Pretreatment preparation visits 
will involve motivational interviewing and cognitive behavioral techniques to increase motivation to quit and 
prepare participants for their quit attempt and treatment.  
4) Counseling. Throughout the treatment phase, all participants will complete weekly or biweekly 
counseling sessions with a therapist trained and supervised in smoking-cessation treatment who will review the 
participant’s quit plan (including target quit date and tobacco use reduction plans) and quit status. As part of this 
assessment participants will complete a Time-Line Follow-back form to assess self-reported smoking behavior 
since their last treatment visit (see Appendix 4). The study therapist will also administer the Varenicline 
Toleration Assessment (Appendix 7) during the counseling session, including sessions that occur after the 
participant has taken their final dose of Varenicline.  Counseling will include cognitive-behavioral approaches 
emphasizing the participant’s understanding of the antecedents and consequences of his or her smoking, and 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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skills training to help the participant cope with situations associated with smoking. Materials that counselors 
may use during sessions or give to participants as take home materials are listed in Appendix 10. One 
counseling session, lasting approximately 30 min, will be scheduled weekly or biweekly. Study therapists will 
receive extensive training and supervision by the study MAI (who may also serve as a therapist as needed).  
Therapists will either be Masters level counselors or hold a PhD in a relevant field (e.g., psychology) 
with prior clinical experience. Those with a Master’s degree or higher in a relevant field but without clinical 
experience may also be trained as counselors, but will complete more extensive training that will consist of 
didactic instruction in principles of cognitive behavioral therapy and cognitive behavioral techniques specific to 
smoking cessation, observation of counseling sessions with a trained counselor, and observation by a trained 
counselor of treatment delivery. The MAI and the lead study therapist will meet with all new counselors for an 
overview of the NRB nicotine cessation counseling program, as well as an in-depth discussion of the theories 
and techniques behind cognitive-behavioral therapy (CBT) and motivational interviewing (MI). Trainees will 
also be provided with a manual which outlines the nicotine treatment program, and discusses the MI and CB 
strategies to be employed at each level of treatment. The individual worksheets and exercises included in this 
manual will be reviewed with the trainee. New counselors will observe a minimum of 3 treatment sessions, and 
will be observed a minimum of 3 times by the lead study therapist or Dr. Salmeron. They will also attend the 
weekly supervision meetings with Dr. Salmeron and the lead study therapist. During the supervision meetings, 
counselors discuss current cases and bring up any concerns or challenges in order to gain direction and insight 
in future treatment applications. Bachelor’s level staff with a background in psychology or related field may 
also be trained to fill in for counseling sessions when the primary therapist is not available. They will complete 
the aforementioned training. 
 
5) Nurse assessment. On the day of their scheduled treatment session participants will meet with a study 
nurse who will assess their vital signs, and administer an expired CO measurement. CO levels of ≥ 4ppm will 
be considered positive. The study nurse will also administer a urine cotinine test on their first visit after self-
reported abstinence and then every 2 to 3 weeks after this, depending on how late in their course of treatment 
they achieve abstinence. Urine cotinine assessments will only be administered if the participant reports 
abstinence for the past 7 days ~15min. 
 
6) Phone Assessment. During weeks where participants are not scheduled for in-person counseling the 
study therapist, LI or study clinician will conduct telephone assessments to monitor for changes in physical and 
psychiatric symptoms (administering the Varenicline Toleration Assessment, Appendix 7) and assess smoking 
status. 
 
7) Questionnaires. During each weekly visit participants will complete the state measures indicated in 
Appendix 4.                               ~15min 
 (vi) Follow-up.  
Participants will be asked to complete follow up visits at 1, 6, and 12 months after their last varenicline 
dose. During these visits participants will complete a nurse assessment (same as nurse assessment outlined 
above for scan visits with a definite urine cotinine test), meet with an investigator to assess quit status and 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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smoking abstinence since last visit, completing state measures indicated in Appendix 4, and complete a 15-20 
min scan session involving the following procedures: 
 
Structural Images. A set of high resolution structural MR images will be collected. The images will be 
acquired using a T1-weighed three-dimensional MPRAGE sequence and will be used for the display of functional 
data.  Completion time: ~5 min. 
 
Resting Scan. An EPI scan to assess resting-state fluctuations (RSF) will be conducted. These scans will 
provide us with information relating to trait and perhaps state connectivity between different prefrontal and limbic 
brain regions. Completion time: ~10 min. 
 
Heart-rate variability and respiration. Heart-rate variability (HRV) will be measured throughout the 
two scanning sessions via MRI compatible ECG electrodes placed on the participant’s chest or back. Resting 
HRV provides a trait measure related to enhanced resilience to stress and affect regulation (Friedman and 
Thayer, 1998; Hansen et al., 2009). Respiration will be measured with a thoracic strain-gauge belt that will be 
fitted around the individual’s waist to control for respiration-related changes HRV.  HRV and respiration will 
be measured during all task and resting functional scans.  
(vi)Potential problems and alternatives 
1) Missing a treatment visit. If a participant cannot attend their scheduled weekly visit, they will be 
offered the next available time within that week. If they cannot attend an appointment that week they will be 
asked to complete their medical monitoring assessment (see Section 8 Subject Monitoring) and counseling over 
the phone at a convenient time that week. 
 
2) Inability to tolerate varenicline. If participants are unable to tolerate varenicline, they can continue to 
attend the scheduled counseling visits and assessments and will be offered nicotine replacement therapy (NRT) 
as an alternative to varenicline treatment. NRT will involve daily application of a dose-matched nicotine 
patch/patches as well as optional 2 or 4 mg nicotine lozenges. Dose matching will be conducted according to 
the following schedule of daily patch administration: 
 
10-15 cigarettes per day: 21mg patch 
16-20 cigarettes per day: 21mg + 7mg patch 
21-25 cigarettes per day: 21 mg + 14 mg patch 
26 or more cigarettes per day: 21mg +21mg patch 
 
3) Other issues or concerns expressed by participants regarding the treatment schedule. If any participant 
expresses concerns or experiences issues regarding attendance at counseling sessions or varenicline 
administration (e.g., no longer wishes to receive varenicline treatment) we will work with the individual 
participant to derive a treatment plan that is suitable for them. This will help participants remain in treatment for 
the full 12-weeks. For example, if any participant wishes to discontinue varenicline treatment but still wishes to 
attend the counseling sessions, they will be offered NRT as an alternative treatment, but may still continue the 
counseling sessions in the event that they refuse NRT. Alternatively, if a participant wishes to continue varenicline 
treatment but has issues/concerns with attendance at counseling sessions, they can continue to receive varenicline 
treatment without counseling. However, we will require that at a minimum they attend NIDA-IRP at least 
biweekly to pick up their weeks supply of varenicline, complete a nurse assessment, complete their questionnaires 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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and receive a brief medical monitoring and abstinence assessment from a study therapist. During the weeks that 
participants do not come in, study therapists will call participants to conduct telephone assessments to monitor 
for changes in physical and psychiatric symptoms (administering the varenicline toleration assessment) and assess 
smoking status. 
 
Participants do not wish to attend follow-up visits. If a participant is contacted at the 1, 6 or 12 month 
follow-up time point and at this point states they do not wish to attend a follow-up visit, they will be asked to 
complete a part of their follow-up assessment over the phone. The assessment will be conducted by one of the 
study therapists who will assess the participants quit status and smoking history since their last contact with the 
study team. 
4.5. End of participation 
 Participation in the study will be terminated once all study procedures including treatment and follow-
up sessions are completed or under circumstances warranting participant withdrawal from the study (see section 
“7. Subject Monitoring”). Participants can also withdraw from the study at any time should they no longer wish 
to participate. Participants who withdraw from the study will be provided with information regarding alternative 
treatment options within the community. If participants decide during the pre-treatment study phase that they 
wish to quit smoking immediately (that is, prior to completing their pre-treatment scan sessions), they will be 
withdrawn from the study and provided information regarding alternative treatment options within the 
community. If participants withdraw during the treatment phase of the study, they will be asked if they wish to 
consent to be contacted at the three follow-up points. They will also be provided with information regarding 
alternative treatment options within the community should they wish to seek treatment elsewhere. Typically, no 
data obtained during the course of this study will be shared with the individual participant or their health care 
providers. 
4.6. Relationship to other protocols. 
All participants will be asked to participate in protocol 10-DA-N457. Participation in protocol 10-DA-
N457 is required for inclusion in the current study. Genotyping and blood draw for genotyping described in the 
current protocol will carried out under protocol 10-DA-N457. Participants who complete the Motivational 
Interviewing Arm of the study (see Section 22) may be offered admission to protocol 13-DA-N485.  
5. Storage of data and samples 
Clinical data will be stored in the Clinical Data Warehouse (CDW) and in clinical research charts or 
research binders.  Research charts or binders will be stored in locked filing cabinet, in a locked room at the NIDA-
IRP.  Imaging data are stored on a password protected drive on a UNIX server, which is maintained by the NRB. 
One copy of the consent form is kept in the participant’s NIDA-IRP medical chart, the other is kept in a limited 
access, locked cabinet with other consent forms from the same protocol.  Summaries of data analyses (e.g. 
demographics, clinical laboratory results, consent audit) are stored on a password protected shared NRB data 
drive.  Participant data contained within files on the NRB data drive are identified by ARC number, and not by 
other personal identifiers such as name.   
Plasma samples acquired for measurement of cortisol and ACTH, will be destroyed once valid assays 
have been obtained. 
 

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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6. Data Sharing Plan 
Unless specified otherwise, data collected in this study may also be combined with data collected across 
IRB approved protocols, including the NRB Repository Protocol, 06-DA-N802 and the NRB protocol, 10-DAN-
457, “Characterization of Phenotypic and Genotypic Regressors to allow for greater power to detect otherwise 
small effect size analyses untestable with a single data set. Additionally, de-identified data may be shared with 
properly administered databases and/or with collaborators with whom proper data sharing agreements are in place. 
 
 
7. Additional Considerations 
N/A 
8. Risks/ discomforts 
8.1. Questionnaire and Characterization Measures.  
The questions asked may be perceived as embarrassing or may cause anxiety or distress.  Nevertheless, 
such reactions are expected to be temporary and to rapidly dissipate spontaneously.  
Risk Minimization.  Participants will be advised prior to completing these measures that they may refuse 
to answer any questions that cause them discomfort.  Furthermore, any participant who experiences anxiety or 
distress as a result of these assessments will be able to speak to a NIDA-IRP mental health professional to discuss 
this.   
 
8.2. Nicotine Patch Administration. 
 
Adverse events (AEs) related to the nicotine patch include: 
 
• 
Abdominal Pain  
• 
Palpitations 
• 
Chest pain  
• 
Rash 
• 
Coughing 
• 
Restlessness  
• 
Diaphoresis 
• 
Sleepiness 
• 
Dizziness  
• 
Temporary mood change 
• 
Dry mouth 
• 
Minor skin irritation 
• 
Headache  
• 
Nausea/Vomiting  
 
 
The most common possible side effects of nicotine patch administration are skin irritation, sleep 
disturbances, and nausea and vomiting.  All effects are temporary and will dissipate spontaneously and quickly 
(1-2 hrs) after patch removal. 
More severe toxic effects of nicotine may include cardiac tachyarrythmias, hypertensive crisis, dizziness, 
weakness, confusion, and convulsions.  The lethal dose of nicotine in adults is from 0.5-1.0 mg/kg or a total single 
bolus dose of 40-60mg (http://www.cdc.gov/mmwr/preview/mmwrhtml/mm5218a3.htm) (US-EPA. Chemical 

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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profile: Nicotine. US Environmental protection Agency 1987).  Nicotine death may likely result from paralysis 
of respiratory muscles or central respiratory failure.  Individuals likely have widely different levels of tolerance 
to the toxic effects of nicotine based on previous exposure.  Nicotine patch doses up to 63mg/d have been shown 
to be safe and generally well-tolerated by smokers (Zevin et al., 1998; Ebbert et al., 2007). Most reports of nicotine 
poisonings and deaths are typically the result of contact with nicotine-containing pesticides (Benowitz et al., 
1987).  Although ingestion of nicotine is common, deaths due to poisoning are extremely rare, due to early 
vomiting and the short half-life of the drug.  In the current protocol, the protracted release of nicotine via the 
transdermal (24hr) patch should keep nicotine plasma concentrations well within a safe range.  If participants 
show initial signs of nicotine poisoning during the patch-toleration test (i.e., burning throat, nausea, vomiting) 
they may be excluded from further participation depending on the severity of their symptoms 
 Risk Minimization. In order to minimize risks associated with nicotine, only individuals who meet certain 
medical history criteria will be recruited into the study. Participants will be clearly instructed on potential side-
effects during their orientation visit and will be provided with a phone number to speak with a research team 
member should they experience any side-effects following patch application. These instructions will be re-iterated 
to all participants over the phone when they are contacted to commence their abstinence periods. They will be 
given identical instructions prior to both the placebo and nicotine-patch abstinence periods. During imaging visits, 
all subjects receiving a nicotine or placebo patch will be monitored for blood pressure, respiratory rate, and pulse 
rate changes prior to scanning sessions and compared to their admission baseline values.  Nicotine or placebo 
patch administration will be terminated immediately upon participant’s request.   
8.3. Varenicline administration. (Applicable to treatment-seeking smokers only) 
Varenicline is being used in accordance with its FDA-approved labeling in this study. The most frequent 
AE reported in varenicline treatment groups during Phase II and III trials were nausea, insomnia, abnormal 
dreams, and headaches (Gonzales et al., 2006; Jorenby et al., 2006; Nides et al., 2006; Oncken et al., 2006).  
Nausea, the most common AE, was reported by ~30-50% of participants receiving varenicline in comparison to 
~10% of those receiving placebo (Glover and Rath, 2007). Reports of nausea are typically described as transient 
and mild to moderate in severity.  Discontinuation rates for varenicline are generally similar to those seen for 
placebo- and buproprion-groups in these trials (e.g., Nides et al., 2006). Titration of doses has been shown to 
reduce the overall incidence of nausea to ~16-34% (Oncken et al., 2006).  Generally, very few participants (i.e., 
< 5%) discontinue study medication because of nausea (Oncken et al., 2006).  A long-term study assessing the 
safety and efficacy of varenicline administration concluded that varenicline can be safely administered for up to 
52 weeks, the duration of that study (Williams et al., 2007).  Based on extant clinical data there appear to be no 
clinically meaningful pharmacokinetic drug-drug interactions and no evidence for human abuse liability (Glover 
and Rath, 2007).  
Other discomforts that have been reported include vomiting, abdominal pain, gas, indigestion, constipation, 
reflux, dry mouth, diarrhea, and mouth ulceration. Additional frequent side effects include difficulty sleeping 
(18%), abnormal dreams and nightmare (13%), headache (15%), feeling fatigued or sleepy (7%), and change in 
appetite (3%). Additional listed side effects include tinnitus (ringing ear), increased urination, blurred vision, 
sweating, hot flush, increase or decrease of blood pressure, increased heart rate or palpitation, flu-like symptoms, 
drug allergy, abnormal liver function test, increase in weight, abnormal electrocardiogram, back and muscle pain, 
disturbance in attention, dizziness or fainting, feeling restless, anxiety, depression or euphoria, irritability, and 
agitation. A complete list of the expected AEs and side effects can be found in the drug insert (see attached 
chantix_pfizer_insert.pdf). In addition, based on Pfizer and FDA's ongoing safety review of post-marketing 
reports, Pfizer recently updated the varenicline label in the U.S. to include a warning that patients who are 
attempting to quit smoking with varenicline should be observed for serious neuropsychiatric symptoms, including 
changes in behavior, agitation, depressed mood, suicidal/violent ideation, and suicidal/violent behavior. Pfizer 

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also stated that a causal relationship between varenicline and these reported symptoms has not been established. 
In some reports, however, an association could not be excluded. More specifically, some reports may have been 
complicated by the symptoms of nicotine withdrawal in patients who stopped smoking, but not all patients with 
these symptoms had quit smoking. Some patients with pre-existing psychiatric illness experienced a worsening 
of their conditions. A recent report compared patients with mental illness and patients with no mental illness who 
were on varenicline treatment for smoking cessation reported that “there was no evidence that varenicline 
exacerbated mental illness” (Stapleton et al., 2008).  
A recent case report describes multiple episodes of severe hypoglycemia experienced by a 51 year old Type-I 
diabetic after the onset of varenicline treatment (Kristensen et al., 2008).  The discontinuation of varenicline 
resolved any further hypoglycemic episodes.  Thus, it is possible that diagnosed/undiagnosed diabetics may 
experience severe hypoglycemic episodes while taking varenicline. In the consent, severe hypoglycemic events 
will be noted as a potential side effect. 
A list of treatment-emergent adverse events reported by patients during all clinical trials involving varenicline 
indicates that cardiac issues (e.g., arrhythmia, myocardial infarct) occurred infrequently (Pfizer insert).  Given 
that cigarette smoking has a negative impact on the heart and the potential for cardiac side effects, in the consent 
cardiac disorders are noted as a potential side effect.  
Risk Minimization. Only individuals who meet certain medical history criteria will be recruited into the study.  
Participants with a history of heart problems, diabetes, or abnormal renal functions will not be included in the 
present study.  Doses will be titrated to reduce the severity/incidence of side effects. The daily dose of varenicline 
may be reduced (on a case-by-case basis) to alleviate side effects such as nausea.  We have excluded individuals 
with current or past major psychiatric illness, and will closely monitor potential psychiatric symptoms, depression, 
and suicidal ideation as part of the clinical monitoring plan.  The monitoring plan (see Section 8 Subject 
Monitoring) consists of weekly in-person assessments at NIDA-IRP during 12 week treatment visits. Drug 
administration procedures will be terminated immediately upon participant request.  In the event of a severe side 
effect such as a hypoglycemic event, myocardial infarction/arrhythmia, or increase in suicidal ideation, 
participants will be advised to immediately discontinue study pill administration. If seeking medical care, 
participants will be instructed to notify their physician or medical care providers of the fact that they are taking 
varenicline. 
8.4. Smoking abstinence/nicotine deprivation 
The 36 hr nicotine deprivation is expected to produce acute nicotine withdrawal symptoms, and hence 
some degree of discomfort. According to the DSM-IV (APA, 1994) acute nicotine withdrawal is associated with 
the following symptoms: 
 
• 
dysphoric or depressed mood 
• 
insomnia  
• 
irritability, frustration, or anger 
• 
anxiety 
• 
difficulty concentrating 
• 
restlessness  
• 
decreased heart rate 
• 
increased appetite 
 
As our primary interest in the current protocol is the neurobiological mechanisms underlying acute 
nicotine withdrawal symptoms, no specific efforts will be made to minimize the discomfort associated with these 

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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symptoms during the participant’s abstinence period or scanning session. However, participants will be fully 
aware that their participation is voluntary and that they can withdraw from the study at any time, including during 
the abstinence period or scanning session. 
8.5. Blood draw 
AEs related to blood draw may include: 
 
• 
Minor Skin irritation 
• 
Pain/Discomfort 
• 
Scarring 
• 
Syncope 
• 
Weakness 
• 
light-headedness 
• 
Bleeding 
• 
Swelling at draw site 
• 
Infection 
 
Risk Minimization. Blood draws will only be performed by experienced medical or nursing staff. Risk of 
Infection is minimized by the use of sterile blood drawing procedures. 
 
8.6. Intravenous Catheter 
Possible side effects of intravenous catheter insertion might include acute pain, infection, phlebitis, 
lightheadedness/fainting or bruising. 
Risk Minimization. Intravenous catheter insertion and blood draw will only be performed by experienced 
medical or nursing staff.  
 
8.7. MRI Scanning.   
When used on appropriately qualified individuals, MRI presents minimal risk as long as technical scan 
parameters remain within FDA guidelines. There is no exposure to x-rays or radioactivity.  The radio waves used 
have produced burns (most of these minor) in about one in a million exams. The magnet may move metal implants 
in the body, the motion of which could be painful and harmful.  Metal implants may also cause burns from the 
radio frequency energy used.  While inside the magnet, subjects may experience an acute panic attack due to 
claustrophobia. Subjects may also experience mild, remittable discomfort from lying in the scanner. 
Risk Minimization.  To ensure adherence to FDA guidelines for MRI, a trained MRI operator who has 
been instructed in these guidelines performs all machine manipulations.  Other preventative measures include 
assuring that all equipment to be used during imaging sessions is MR compatible, that participants are familiar 
with the MRI environment (using the mock scanner), and that participants are aware of how to signal the MR 
operator if they need to do so during the session.  Furthermore, participants are screened prior to each MRI session 
for any MR contraindications, including metal implants, pregnancy, fear of small, enclosed spaces, and inability 
to lie still for prolonged periods of time.    

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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8.8. EEG Recording.   
When recording EEG an elastic cap containing electrodes is placed on the participants head.  During 
preparation conductive gel is inserted into each electrode cup and worked into place using the blunt end of a 
cotton swab. Subjects may experience mild scalp irritation during the preparation process.  Extended periods of 
time (typically > 6hrs) wearing the EEG cap may produce tension headaches. Some people may experience 
tension headaches after shorter periods of time, particularly if the cap fits tightly around their heads. Participants 
will need to wash the conductive gel from their hair at the end of the recording session. 
Risk Minimization.  All EEG preparation was conducted by Meredith McHugh, who has extensive 
experience conducting EEG research.  Participants will not wear the EEG cap for more than 6 hrs (typically no 
more than 3 hours) and can ask to have it removed at any time if too uncomfortable. Participants will be informed 
of the EEG clean-up process before enrolling. 
8.9. Physiological monitoring.  
The tape (or electrodes) used to attach the wires may cause minor skin rash.   
Risk minimization. If this happens, a decision will be made by the MAI about whether the participant 
should complete this part of the experiment. 
8.10. 
 Tasks completed inside and outside of the scanner.   
Participants may find the tasks that they are asked to perform during, before and after scanning sessions 
boring, difficult, and/or frustrating. 
Risk Minimization.  In order to reduce discomforts associated with performing the emotional and 
cognitive tasks, scanning sessions and task sessions outside of the scanner are organized in such a way that 
participants will not perform tasks for extended periods of time and will be given a chance to rest between tasks.  
In addition, the tasks are designed to make performance neither too difficult nor too easy. Ideally, individual 
adjustments of task difficulty enable participants to remain actively engaged in the task.  Participants receive 
extensive training before the actual experimental task begins. 
8.11. 
Presentation of smoking-related pictures.   
This protocol includes several procedures which involve the presentation of smoking-related pictures 
which are likely to induce or increase craving/urge to smoke and may also cause some mild distress.  
Risk minimization. The craving/mild distress elicited by these procedures is a primary outcome measure 
of interest and central to the manipulation. There will thus be no effort to minimize the craving/mild distress 
experienced by participants. However, participants will be made fully aware during the consent process that they 
have the right to discontinue this procedure without consequence at any time. 
8.12. 
Self-report characterization and state measures  
Some questions asked may be perceived as embarrassing or may cause anxiety or distress.  Nevertheless, 
such reactions are expected to be temporary and to rapidly dissipate spontaneously.   
Risk Minimization. Participants will be advised prior to completing these measures that they may refuse 
to answer any questions that cause them discomfort.  Furthermore, any participant who experiences anxiety or 
distress as a result of these assessments will be able to discuss it with a NIDA IRP mental-health professional. 

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8.13. 
Electric shock.    
Electric shock as administered in the proposed study poses minimal risk to participants and some 
discomfort which is similar to that of having a tight rubber band snapped against the skin. 
Risk Minimization. Risk of discomfort is minimized by employing the shock workup procedure described 
in section 4.4 Study Procedures. During the consent process and again after the shock workup procedure, 
participants are explicitly informed of their right to discontinue the procedure without consequence at any point. 
8.14. 
Electric shock within the MRI scanner.   
Introduction of electrical wires that are connected to the participant in the strong magnetic field of the 
scanner may constitute an additional risk.  The main risk associated with administering shocks is the introduction 
of electrode wires directly in the RF (radiofrequency) field of the magnet.  To minimize this risk, wires will not 
be exposed to the radiofrequencies induced in the RF head coil.  The shock stimulator equipment will be outside 
the magnetic area, and a plastic and copper cable is taken through the wall for connection to the participant 
electrodes.  To eliminate the possibility that electrode wires will enter the RF-field from participant movements, 
shock electrodes will be attached to either the foot or ankle region.  Area between the wires will be minimized by 
twisting the wires together, and coiling of the wires will be prevented by inspection prior to each session (i.e., 
wires run without loops). MRI compatibility of all devices/wires entering the magnetic field of the scanner is 
determined by Thomas Ross, PhD, a staff scientist here at NIDA-IRP. Dr. Ross has a doctorate in Experimental 
Plasma Physics and has extensive experience in the acquisition and analysis MRI data and in the development of 
MRI compatible procedures.  
 
8.15. 
Acoustic Noise.   
Some subjects may experience temporary, reversible shifts in hearing threshold after MRI. 
Risk Minimization.  The sound generated by an MR system usually consists of a series of repetitive pulses. 
Acoustic noise is a result of the mechanical vibration produced by the gradient coils when the large currents are 
applied to them to create time varying imaging gradient fields. The relevant safety parameters required to 
characterize such a noise are the peak impulse sound pressure level (L-peak) and the time integral of the A-
weighted sound pressure level (Leq).  In MR applications, the peak impulse sound pressure level is dependent 
upon the peak amplitude of the individual pulses, while the time integral of the A-weighted sound pressure level 
is dependent upon the continuous exposure to a series of such pulses. Based on Occupational Safety and Health 
Administration (OSHA, Occupational Noise Exposure – 1910.95, www.osha.gov) regulations: 1) exposure to 
impulsive or impact noise should not exceed 140 dB peak sound pressure level, and 2) exposure to continuous 
noise for 2 hours per day should not exceed 100 dB. Note that these regulations are based on employees’ daily 
exposure to a noisy environment over their entire working career, whereas we use them here as a comparison for 
participants’ occasional exposure to MRI scanner noise, thus making these values extremely conservative 
measures. Measurements within our MRI scanner (provided by Siemens, the manufacturer) indicate that the 
maximum noise level produced by our standard fMRI pulse sequence is 116.5 dB. Based upon our typical echo 
spacing, this sound is in the 3-4 kHz range. 
According to the Occupational Safety and Health Administration regulations (OSHA, occupational noise 
exposure 1910.95, www.osha.gov): 
1) Exposure to impulsive or impact noise should not exceed 140 dB peak sound pressure level, and  
2) Exposure to continuous noise for 2 hours per day should not exceed 100 dB.  
These guidelines are based on employees' daily exposure to a noisy environment over their entire working 
career, whereas we use them here as a comparison for participants' occasional exposure to MRI scanner noise. 

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This study consists of a series of scans ranging in length from a few seconds to about ten minutes. Rest periods 
of up to a few (1-5) minutes occur regularly between scans while the next scan is being set up.  
During fMRI experiments, MRI compatible headphones with tubes for audio input and/or earplugs with 
or without tubes for audio input are routinely used for hearing protection. In addition, a vacuum pillow or other 
padding used for head stabilization may also attenuate noise. Based on the manufactures specifications, the 
earplugs with and without audio tubes have noise reduction ratings of approximately 25dB and 33 dB. The 
headphones have a noise reduction rating of 29 dB. The vacuum pillow / padding foam provides additional noise 
reduction (values not available, but estimated at 5-15 dB). The noise reduction rating is a conservative measure 
that averages over frequencies and attempts to account for improper protection use. The mean noise attenuations 
at 3150 Hz, a frequency near that of our scanner, are actually 35dB, 47dB for the 2 types of earplugs and 40dB 
for the headphones used. Thus, the use of any two of these devices in combination reduces the noise level to levels 
much lower than that required by OSHA for lifetime exposure, and provides effective hearing protection for the 
participants. Nevertheless, some subjects may experience temporary, reversible shifts in hearing threshold after 
MRI. 
8.16. 
Mock Scanner.   
Potential side effects associated with the mock scanner include mild backache from having to lie still for 
a prolonged period of time, temporary difficulty hearing soft sounds after the exam, and being uncomfortable 
being in a small enclosed space.  
Risk Minimization.  Pre-study screening for history of back problems or claustrophobia will minimize the 
likelihood of side effect from mock scanning.  In addition, participants will be given adequate hearing protection 
to minimize hearing difficulties resulting from mock scanning procedures (see Acoustic Noise section above for 
details).   
9. Subject monitoring 
9.1. Parameters to be monitored 
 
Participants will complete a nicotine patch toleration test during the orientation visit. Nursing staff will 
apply the dose-matched nicotine patch at the end of the nurse assessment and will describe potential side effects 
to the participant. Participants will wear the patch for approximately 3 hours and will be asked to notify the LI or 
nursing staff immediately if they experience any worrisome side-effects. 
Participants will be instructed during their orientation visit, and on the phone immediately prior to the 
commencement of each abstinence period, on the potential side effects of nicotine administration via transdermal 
patch. The details of what side effects are possible, when to remove the patch, and details of who to contact should 
participants experience side effects are clearly outlined in the Nicotine Patch Instructions (Appendix 5) which 
participants will take home with them from the orientation session. During telephone and in-person assessments 
participants will be directly asked open-ended questions will be used to assess for the presence of adverse effects. 
During placebo-patch and nicotine scans heart rates will be monitored via pulse oximeter. After completion of 
the scan, nursing will remove the patch and measure blood pressure and heart rate.  
Based on Pfizer and FDA's ongoing safety review of post-marketing reports, Pfizer have modified the 
varenicline label in the U.S. to include a warning that patients who are attempting to quit smoking with varenicline 
should be observed for serious neuropsychiatric symptoms, including changes in behavior, agitation, depressed 
mood, suicidal ideation, and suicidal behavior. However, a recent cohort study based on the UK General Practice 

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Research Database (N = 80, 660) found no significant difference between varenicline and nicotine replacement 
therapy in reported incidents of fatal and non-fatal self-harm (0.16% vs 0.22%), suicidal ideation (0.05% vs 
0.05%) or initiation of anti-depressant use (3.18% vs 3.62%) (Gunnell, Irvine, Wise, Davies, & Martin, 2009). 
Nevertheless, to help mitigate potential risks related to changes in mood, behavior, or suicidal ideation and to 
assess for all side effects, a clinical monitoring plan involving weekly in-person assessments during the 12-week 
treatment period will be implemented.  During their initial treatment interview (the day they set their quit date) 
or during their final pretreatment visit they will briefly meet with the study MAI or the designated NIDA-IRP 
physician in the MAI’s absence who will distribute a 2 weeks supply of varenicline and carefully describe the 
medication, prescribed dosing schedule and possible side-effects. Participants will also receive a set of take-home 
instructions and information sheet (see Appendix 8). During each weekly visit across the entire 12-week treatment 
participants will receive a nurse assessment to assess vital signs and complete a series of questionnaires (see 
Appendix 4). During their counseling session the therapist will administer a semi-structured interview (see 
Appendix 7) to assess for psychiatric and physical symptoms. If participants are unable to attend their weekly 
counseling session in person, study therapists, LI or study clinician will conduct telephone assessments to monitor 
for changes in physical and psychiatric symptoms (administering the varenicline toleration assessment, Appendix 
7). The same assessment will also be conducted each week for the first two weeks after their last Varenicline 
dose. At any time during the study, endorsement of one or more items listed in Appendix 7 will be referred to the 
study MAI or a NIDA-IRP clinician in the MAIs absence to who will determine if the endorsement requires 
further action. Participants with suicidal ideations, atypical changes in behavior, or experiencing clinically 
significant or difficult to tolerate side effects may be provided with a referral, instructed to stop taking the study 
pills, and/or be withdrawn from the study.   
Study therapists will be extensively trained and supervised in administration of the semi-structured 
interview by the study MAI.  The participant will be referred to the MAI if any questions or issues arise. 
At each visit to NIDA, participants are queried about their general medical and emotional health 
(including current medications and drug use history) since their last visit to NIDA and are observed throughout 
each visit at NIDA by study investigators. Any change in health status is noted by protocol staff and evaluated 
for relevance to the participant’s participation. Investigators will refer the participant to the MAI for any medical 
adverse events or questions about recent medical conditions. The MAI will be informed of and respond to any 
adverse events. 
(1) All female participants will have a urine pregnancy test performed before each visit that requires an MRI. 
(2) All participants participating in an MRI study will receive a comprehensive screening for metal and other 
possible devices within the body that are contraindicated in the MRI environment.  
(3) The MRI is for research purposes only and not the type used to look for neurophathological changes; they 
will not be routinely reviewed for such problems. However, sometimes the MRI will show what could be 
an abnormal finding. In this case, the participant will be referred to the proper expert(s) for further 
diagnostic procedures.  
 
Medical Emergencies. During fMRI sessions, which will take place at NIDA-IRP arrangements are in place for 
the emergency transport of subjects to the Johns Hopkins Bayview Emergency Department, if required.  

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9.2. Criteria to be used 
Participants who experience a serious expected or unexpected adverse event (i.e., require hospitalization, 
experience significant disability/incapacitation, death, or have a life-threatening reaction) with likely or greater 
relationship to the experimental procedures will be excluded from any further participation.  Participants may 
also be withdrawn from the study should they experience a serious allergic reaction to the nicotine or placebo 
patch adhesive. Should participants fail to comply with the 36 hr smoking abstinence, they will be asked to 
reschedule their scanning visit and recomplete the 36 hr abstinence. If they do not wish to reschedule, they will 
be withdrawn from the study.  
No specific data on the toxic dose of varenicline appear to be available.  The toxic dose of varenicline 
may be dependent on previous experience with nicotine.  Specifically, smokers are able to tolerate higher single-
doses of varenicline than non-smokers (Faessel et al., 2006a). Varenicline is well tolerated in single doses up to 
3mg (Faessel et al., 2006a) and in multiple doses up to and including 2mg daily (Faessel et al., 2006b) with nausea 
and vomiting being limiting factors.  Participants may be discontinued from varenicline treatment and offered 
alternative treatment (i.e., nicotine replacement therapy) depending on the severity of varenicline related side 
effects. The daily dose of varenicline may be reduced (on a case-by-case basis) to alleviate side effects and 
increase retention rates if necessary. 
 
9.3. Criteria for individual subject withdrawal 
 
Individual participation will be terminated if the individual no longer wishes to participate, is continually 
non-compliant (repeatedly fails to show for appointments without calling to reschedule, does not 
complete procedures as instructed, does not comply with restrictions such as limited caffeine use, drug 
use), if they no longer meet study inclusion criteria, or if a circumstance arises that makes continued 
participation unsafe or ill-advised (for example, an inability to tolerate nicotine patch; participants will 
be allowed to continue in the study without varenicline if they cannot tolerate it). Subjects will also be 
withdrawn if they do not complete the following required procedures: 
1) the two pre-treatment scan visits;  
2) set a quit date;  
3) attempted to quit for at least 24 hours;  
4) we have been able to establish their quit status (i.e., relapsed vs non-relapse) at 1, 6 OR 12 months 
following their last treatment visit. 

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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10. Outcome measures 
10.1. Primary outcomes measures 
1)  Change in BOLD signal and FC related to task parameters, between drug conditions: will provide 
important insight into the neurobiological mechanisms underlying acute nicotine withdrawal, in particular 
those related to anhedonia, negative affect, inhibitory control and impulsive decision making. 
  
2)  Behavioral performance on each of the tasks assessing inhibitory control processes, reward 
responsiveness, amygdala, striatal, BNST reactivity and impulsive decision making (e.g., reaction time, 
error rate, hit rate, reward bias): will provide task-related parameters necessary for analysis of BOLD 
data. Performance data will function as a secondary outcome by providing behavioral validation of acute 
nicotine withdrawal effects.  
 
3)  Self-reported craving, withdrawal symptoms and mood/affect: will be employed as regressors in the 
analysis of task and resting BOLD data.  They will also provide the primary means of validating the acute 
nicotine withdrawal manipulation.  
 
4) Smoking status at 1, 6 and 12 months: Smoking status (relapse vs abstinent)  at each of the follow-up 
time points will be based on 7-day point prevalence defined as no smoking (not even a puff) or use of 
any tobacco products for the past 7 days. This is a standard method of assessing abstinence and 
dichotomizing relapse status at follow-up (Keating & Siddiqui, 2006; Leventhal et al., 2008; Swan et al., 
2010b). Whenever abstinence at follow-up is assessed in-person, self-reported abstinence will be 
corroborated with breath CO and urine cotinine levels.  
 
10.2. Secondary outcome measures include:  
4) MRS for glutamate concentration: Glutamate concentration at the PFC and hippocampus is expected to 
change as a function of acute nicotine withdrawal and may also play a role in the expected reduction in 
VS reactivity and anhedonia. 
 
5) Plasma ACTH and cortisol: are expected to increase as a function of acute nicotine withdrawal and is 
expected to play role in both anhedonia and negative affective symptoms, potentially by acting at the 
amygdala, BNST and VS.  
 
6) Resting state CBF from ASL: To test for changes in neural activity (i.e., not connectivity) at rest it is 
necessary to employ a measure which does not require a baseline. CBF provides such a measure as 
changes in blood flow are referenced to a common arterial location outside of the brain. 
 
7) ERP and EEG measures: Secondly, ERP and EEG indices will provide information about neural 
processing that cannot be derived from the BOLD signal and will be important to dissociating temporally 
tightly coupled processes underlying inhibitory control.  
 
 

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8) Ratings and scores on self-report characterization measures: will be employed to examine potential 
mediation of primary hypothesized effects. E.g., does degree of nicotine dependence mediate craving 
related changes in rsFC in the DMN?   
 
9) Structural MRI and DTI data: will be employed to examine potential mediation of primary hypothesized 
effects. E.g., does increased WM integrity between the rACC and amygdala effect rsFC between these 
two structures and in turn amygdala reactivity?  
 
Resting state MRI at follow-up. Resting state MRI will be assessed between- and within-groups as a 
function of relapse status and time since last treatment visit at each of the three follow-up time-points (1, 6 and 
12 months). This will allow us to address the following exploratory questions: 
 Q1. What characteristics of rsFC are associated with treatment success (vs failure) at 1, 6 and 12 months 
post-treatment? 
 
Q2. Are there characteristics of rsFC that vary as a function of time post-treatment in successfully 
abstinent individuals? 
 
11. Statistical Analysis 
11.1. Pre-processing 
The imaging data will be pre-processed with AFNI (Analysis of Functional NeuroImages, 
http://afni.nimh.nih.gov/afni/ (Cox, 1996)) and Brainvoyager 1.7 (BrainInnovation, Masstricht, The Netherlands). 
All functional images will be directly registered upon high resolution MPRAGE anatomical scans obtained during 
the same imaging session. Location and intensity of activations from individual and/or grouped data will be 
translated into 3D stereotaxic coordinates (Talairach and Tournoux, 1988).  Functional images of activation-
induced BOLD signal changes will be determined using cross correlation or multiple regression analyses 
(Worsley and Friston, 1995; Bandettini and Wong, 1997).  
Glutamate concentrations derived from MRS will be quantified for the PFC/ACC and or hippocampus 
with LCModel software. The LCModel software utilizes a library of reference spectra and a curve fitting 
algorithm to estimate the tissue concentration of neural metabolites. Values are given in institutional units 
approximating millimolar (ppm) concentration.   
EEG/ERP data will be pre-processed and analyzed using commercially (BrainVision Analyzer) and freely 
available (Delorme and Makeig, 2004) software packages. Individual and group ERPs will be calculated and the 
amplitude of selected components (e.g., ERN, P300, evoked theta). BESA (Brain Electrical Source Analysis, 
MEGIS, Germany) will also be used to identify the putative neural generators of ERP components using dipole 
source models. 
11.2. Data-Analysis 
Unless otherwise specified, all BOLD, genetic, behavioral, self-report and physiological data will be 
analyzed with AFNI or R (http://www.R-project.org; R Development Core Team, 2008). The types of statistical 
analysis to be utilized in the current protocol are summarized below.  
Nicotine deprived vs non-deprived. Between condition (nicotine-deprived vs non-deprived) comparisons 
will be conducted on self-report, behavioral, BOLD ROI, resting CBF and ERP responses with a series of one-
way repeated measures ANCOVAs, with nicotine-deprivation (nicotine-deprived vs non-deprived) as the within-
subjects factor and nicotine dose as the covariate. By controlling for nicotine dose we can partially dissociate the 

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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effects from acute nicotine-withdrawal effects. Treatment seeking status (Treatment seeking vs non-treatment 
seeking) will be included as a between-subjects factor in these models to assess the main effects and of motivation 
to quit as well as any interaction between motivation to quit and acute nicotine withdrawal. 
Mediation. Where mediation has been hypothesized (e.g., central CRF as a mediator of VS reactivity 
following acute nicotine withdrawal), regression coefficients for the paths of each mediation model will be 
derived with multiple regression and the mediation effect will be tested with ProdClin (Mackinnon, Fritz, 
Williams & Lockwood, 2007). ProdClin is a program developed by MacKinnon, Fritz, Williams, and Lockwood 
(2007) that uses the distribution of the product of paths a and b to compute asymmetric confidence intervals for 
the mediated effect. ProdClin is an alternative to the Sobel (1982) method that provides a more powerful and 
accurate test of the mediation effect because it is based on an asymmetric distribution which more closely 
resembles the true distribution of the product terms. Mediation models will be conducted separately for treatment-
seeking and non-treatment seeking smokers. 
Functional Connectivity. Seed regions for FC will be based on peak BOLD responses in ROIs for the 
parameter of interest. Strength of functional connectivity will be measured by correlating the BOLD time-series 
between seed and target regions. Fisher r to z transformations will be conducted to test for significant differences 
in functional connectivity strength between the non-deprived and nicotine-deprived conditions. Treatment 
seeking status (Treatment seeking vs non-treatment seeking) will be included as a between-subjects factor in these 
models to assess the main effects and of motivation to quit as well as any interaction between motivation to quit 
and acute nicotine withdrawal. 
Correlational analyses. Bivariate partial correlations controlling for nicotine dose will also be conducted 
between self-report and behavioral indices of acute nicotine withdrawal and BOLD indices. Correlational 
analyses will serve to identify neural markers of responsivity to nicotine replacement. A series of bivariate partial 
correlations (controlling for nicotine dose) will also be conducted between BOLD, EEG, psychophysiological 
and neuroendocrine markers between nicotine-deprived and non-deprived conditions. Correlations will be 
conducted separately for non-treatment and treatment seeking groups. 
Genotype by nicotine-deprivation interaction. 2 x 2 mixed-design ANOVAs will be conducted with 
genotype (short vs long allele of the 5-HTTLPR gene) varied between-subjects, and nicotine-deprivation (nicotine 
deprived vs non-deprived) varied within-subjects. The DVs will be BOLD signal change and functional 
connectivity. Treatment seeking status (Treatment seeking vs non-treatment seeking) will be included as a 
between-subjects factor in these models to assess the main effects and of motivation to quit as well as any 
interaction between motivation to quit and acute nicotine withdrawal. 
RSN dynamics under acute nicotine withdrawal. The presence of three independent RSNs resembling the 
ECN, DMN and SN will be confirmed by applying an Independent Component Analysis (ICA) to the band pass 
filtered (0.01 to 0.08 Hz) resting BOLD time-series. ICA analysis will be conducted using the FSL Melodic ICA 
software (http://www.fmrib.ox.ac.uk/analysis/research/melodic/). The time-series of six seed regions reflecting 
core-nodes within each of these networks (ECN: dlPFC, PPC; DMN: vmPFC, PCC; SN: dACC, anterior insula) 
will then be regressed against each other to assess within and between network rsFC. Fisher r to z transformations 
will be applied to assess changes in rsFC between nicotine-deprived and non-deprived conditions. Bivariate 
partial correlations (controlling for nicotine dose) will also be conducted between the degree of change in rsFC 
within/between the three networks and changes in self-reported affective/cognitive withdrawal symptoms/urge to 
smoke as well as changes in working memory and attention indices. Treatment seeking status (Treatment seeking 
vs non-treatment seeking) will be included as a between-subjects factor in these models to assess the main effects 
and of motivation to quit as well as any interaction between motivation to quit and acute nicotine withdrawal. 
Correlations will be conducted separately for non-treatment and treatment seeking groups. 

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smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Relapse vs non-relapse group comparisons. Neurobiological processes underlying acute nicotine 
withdrawal will be assessed as a function of relapse status at each of the follow-up time-points by conducting 
between group comparisons. 
11.3. Criteria for significance 
For behavioral, EEG/ERP and self-report measures alone, a statistical effect exceeding an α-level of 0.05 
will be considered statistically significant. The appropriate adjustments for multiple comparison follow-up tests 
(e.g., Bonferroni, Tukey, Scheffe) will be employed where necessary. For the fMRI analysis, where ROIs are 
stipulated a priori, the threshold for statistical significance will also be set at an α-level of 0.05. For whole-brain 
analyses, probability thresholding of individual voxels is used in combination with minimum cluster size 
thresholding to increase statistical power whilst minimizing the chance of type 1 errors. The underlying principle 
is that true regions of activation will tend to occur over contiguous voxels whereas noise has much less of a 
tendency to form clusters of activated voxels. Although tables of false detection probability vs. cluster size have 
been published, they are limited to 2D images, and are not directly applicable; even if tables were published, they 
could not cover every possible combination of experimental parameters. Therefore, we empirically investigate 
the tradeoff between probability and cluster threshold to achieve the desired significance level for a particular 
experimental condition.   
The AlphaSim program (within AFNI) is a Monte Carlo simulation technique that provides a method for 
multiple comparison correction while persevering statistical power. Using random image generation, Gaussian 
filtering (to simulate voxel correlations), thresholding, and tabulation of cluster size frequencies, the program 
generates an estimate of the overall significance level achieved for various combinations of probability and cluster 
size thresholds assuming spatially uncorrelated voxels.  Overall, our simulations indicate that by accepting a 
minimum cluster size it is possible to obtain an order-of-magnitude improvement in probability threshold over 
the value of probability threshold required if cluster size thresholding is not used. An approximately 200-400 µl 
minimum cluster size will generally be used in these studies to maintain our alpha level of 0.05.The criteria for 
significance (alpha) will be 0.05. 
11.4. Power analysis  
General fMRI power. Since fMRI is the main outcome measurement utilized in the present protocol, the 
key power analysis pertains to the fMRI data.  However, prospective power analyses for fMRI data are 
complicated for several reasons. First, fMRI data are analyzed in a hierarchal manner such that both the intra-
participant variance from the time-course data and the inter-participant variance across individuals could affect 
statistical power. A large number of time points tend to mitigate effects of intra-participant variance, but temporal 
autocorrelation and scanner limitations limit the number of independent measurements per unit time and thus the 
number of independent time points that are collected.  In addition, effect sizes and both types of variance will 
vary spatially.  Because of this, a given study may have sufficient power to detect differences in some brain 
regions, but lack sufficient power in other regions where the null hypothesis is false. Finally, fMRI analysis 
consists of a very large number of non-independent multiple comparisons, greater than 1.5 million at the group 
level, necessitating correction methods less severe than a Bonferroni correction, as discussed above. Thus, a 
proper power analysis on fMRI data requires simulating all of these effects. Desmond and Glover (2002) have 
performed such a simulation.  They show for a relatively modest signal change of 0.5% during a cognitive task 
and with an intra-participant standard deviation of 0.75% and an inter-participant standard deviation of 0.5%, that 
11 participants are required for a power of 0.8 using p < 0.05.  Using a false positive rate of p < 0.002, a level 
more consistent with a cluster size threshold to correct for multiple comparisons (Forman et al., 1995), and with 
the variances kept the same, approximately 21 participants are needed for an expected signal change of 0.5% and 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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11 participants for a signal change of 0.75%.  For more than ~100 independent time points, power (and hence the 
intra-participant variance) is relatively independent of the number of time points (Desmond and Glover, 2002).  
All current analyses should fall within this range. 
Relapse vs non-relapse group comparisons. Based on previous studies comparing individuals who relapse 
following smoking cessation treatment to those who remain abstinent (Janes et al., 2010; Versace et al., 2011), 
we anticipate a medium-large effect size (Cohen’s d = 0.65) when comparing relapsers vs non-relapsers across 
each of our neurobiological outcome measures. As we have proposed clear directional hypotheses regarding the 
relationship between treatment outcomes and neurobiological changes resulting from acute nicotine withdrawal, 
a one-tailed t-test model was used to calculate power.  A power level (1-β) of 0.8 was selected to achieve an 
appropriate balance between the ability to detect significant differences and the number of participants needed.  
Approximately 30 participants per group will be required to detect significant differences between these two 
independent groups at a nominal α level of 0.05 in a one-tailed test assuming a medium-large effect size 
(calculated by G*Power, http://www.psycho.uni-duesseldorf.de/abteilungen/aap/gpower3/). 
Nicotine-deprived vs non-deprived condition comparisons. Estimating a dose-matched nicotine vs 
placebo effect size in 36 hr deprived smokers is complicated by several factors. Firstly, previous within-subject 
nicotine vs placebo studies have employed significantly shorter abstinence periods (e.g., 3 - 4 Hahn, Hong, Cole, 
or 8 hrs, Cole and Beaver). Secondly, studies addressing acute nicotine withdrawal effects using longer abstinence 
periods (e.g., 24 hrs) typically compare smoking deprivation to a smoke adlib condition (Azizian et al., 2010b; 
Field, Santarcangelo, Sumnall, Goudie, & Cole, 2006b; Kozink, Kollins, & McClernon, 2010b; McClernon et al., 
2008b; McClernon, Kozink, Lutz, & Rose, 2009a; Mitchell, 2004b). Following 8 hrs of smoking deprivation, 
nicotine (relative to placebo) produces small to medium effects on self-report withdrawal symptoms, mood and 
craving measures (Cohen’s d = 0.15 to 0.52), medium effects on behavioral measures (d = 0.48 to 0.618) (Beaver 
et al., 2011; Cole et al., 2010). Effects of 12 to 24hrs of smoking deprivation (relative to adlib smoking) on self-
reported craving and affect/mood vary from medium to large (d = 0.51 to 4.91). The same deprivation periods 
produce small to medium effect sizes (d = 0.23 to 1.435) for go-nogo, attention and delay discounting tasks with 
larger effects on RT and discounting rate and smaller effects on error rates.  
In the current study we aim to increase the severity of nicotine-withdrawal effects by employing a 36 
hour deprivation period. At the same time, individual differences in responsivity to nicotine replacement will 
likely reduce between-condition effects relative to smoking deprivation vs smoke adlib studies cited above. 
Together the above studies suggest that the weakest effects of a dose-matched nicotine vs placebo manipulation 
in 36 hr deprived smokers smoking deprivation manipulation will arise for behavioral measures and will be 
medium in size (d = 0.5). We selected a power level (1-β) of 0.80 to achieve an appropriate balance between the 
ability to detect significant differences and the number of participants needed. Assuming an alpha-level of 0.05, 
a correlation among repeated measures of r = 0.5 and a medium effect size, approximately 35 participants will be 
required to achieve a power of 0.80 (calculated by G*Power http://www.psycho.uniduesseldorf.de/abteilungen/ 
aap/gpower3/).  
Motivation to quit by nicotine-deprivation. To generate an estimated effect size for a 2 (treatment seeking 
vs non-treatment eeking) x 2 (deprived vs non-deprived) we referred to the single study conducted to date 
comparing treatment seeking smokers to non-treatment seeking smokers (Wilson et al., 2012). In this study, which 
employed self-report physiological and neuroimaging outcome measures, the smallest effect size reported for a 2 
x 2 between-within interaction, where the between subjects factor was treatment seeking status, was η2= .08. 
Based on an effect size of η2= .08, and a power of 0.80, and an estimated correlation between repeated measures 
of 0.5, the required sample size to detect a true 2 x 2  between-within interaction effect is 26 individuals. 
Genotype by nicotine-deprivation. To derive an estimated effect size for the genotype by nicotine-
deprivation interaction we examined effects of the 5-HTTLPR polymorphism on pharmacological manipulations 
where conditions are varied within-subjects. Genotype by treatment effects on behavioral and self-report outcome 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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measures in these studies display a  small to large effect size (d = 0.30 to 0.90) (Kellner et al., 2009; Marsh et al., 
2006; Thakur, Grizenko, Sengupta, Schmitz, & Joober, 2010). Recently, (Minnix et al., 2011) reported a large 
effect (d = 1.45) of  the 5-HTTLPR polymorphism on startle reactivity in overnight deprived smokers following 
nicotine vs placebo nasal spray. Although the effect of the serotonin transporter polymorphism on brain (BOLD 
or EEG/ERP) activity changes following nicotine or smoking deprivation has yet to be investigated, large effect 
sizes (d > 0.80) have been reported for a smoking deprivation by COMT genotype interaction (Wang et al., 2008).  
Due to the large variance in reported effects we conducted two power analyses assuming a medium (d 
=0.50) and large effect size (d = 0.80) effect size. Based on these analyses and assuming a correlation among 
repeated measures of r = 0.5, we would require approximately 16 to 35 individuals to achieve an 80% chance of 
detecting a genotype by nicotine-deprivation effect in the current study across our BOLD and behavioral indices 
(calculated by G*Power, http://www.psycho.uniduesseldorf.de/abteilungen/aap/gpower3/).  
Correlational. Bivariate partial correlations (controlling for nicotine dose) will be conducted between the 
change in BOLD, ERP/EEG, psychophysiological and neuroendocrine markers, behavioral and self-report under 
nicotine-deprivation (relative to nicotine satiety). Large effects (d > 0.80) are typically observed between changes 
in self-report (e.g., craving, withdrawal symptoms) and BOLD measures (e.g., insula or amygdala activity or 
functional connectivity) during smoking abstinence relative to smoke adlib (Cole et al., 2010; Kozink et al., 
2010a; McClernon et al., 2009b). Similarly large effect sizes are reported between changes in behavioral indices 
(e.g., RT variability) and BOLD responses (e.g., PCC task response) under nicotine (relative to placebo) in 
minimally deprived smokers (Hahn et al., 2007). Large effect sizes also arise between BOLD responses and 
neuroendocrine and psychophysiological markers (Ahs, Sollers, Furmark, Fredrikson, & Thayer, 2009; Drevets 
et al., 2002) as well as between behavioral indices and time-to-smoke in the laboratory relapse model (Dallery & 
Raiff, 2007). Based on a slope of 0.59 between each predictor and outcome measure (a large effect size according 
to J. Cohen, 1988) a sample size of at least 20 would be required to achieve a power of 0.80. (Calculated by 
G*Power, http://www.psycho.uniduesseldorf.de/abteilungen/aap/gpower3/).  
Mediation Analyses. Based on the literature reviewed above, we expect a large effect size between each 
predictor, outcome and mediation variable in the mediation models described above. Based on a large effect size 
for paths a and b, Fritz and McKinnon (2007) suggest a sample of 35 to achieve a 0.80 power to detect each 
mediation effect with Prodclin.  
11.5. Accrual number request, taking into account screening/ dropouts   
Treatment-seeking smokers. In the current study, an individual will be considered a completer if they 
meet the following criteria: 1) have completed the two pre-treatment scan visits; 2) have set a quit date; 3) 
attempted to quit for at least 24 hours; 4) we have been able to establish their quit status (i.e., relapsed vs non-
relapse) at 1, 6 and 12 months following their last treatment visit. Based on the power analyses above we anticipate 
that a minimum group size of 30 in each of our two relapse groups (relapse vs abstinent) at each follow-up time 
point (1, 6 and 12 months)  would provide a reasonable level of statistical power allowing for the detection of 
effects of interest in the present study. Based clinical trials of varenicline with similar follow-up time points 
(Aubin et al., 2008; Keating & Siddiqui, 2006; Krebs & Sherman, 2012; Swan et al., 2010a; Tsai et al., 2007), we 
estimate that 35% of completers at 12 months will meet criteria for 7-day point prevalent abstinence. The 12 
month follow-up is the point at which we would expect our abstinent group to be smallest. Achieving a minimum 
of 30 abstinent smokers at 12 months would require 85 completers (i.e., 35% abstinence rate). Based on an 
expected 30% attrition rate at 12 months (Keating & Siddiqui, 2006) a total of 120 treatment-seeking smokers 
(85 completers) is therefore requested.  
Non-treatment seeking smokers. An individual will be considered a completer if they complete 
procedures outlined for Phase 1 of the protocol (see Section 4 Study Design and Methods). Based on power 
analyses described above and in Section 10.4 we anticipate that 35 non-treatment seeking smokers with complete 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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and useable data would provide a reasonable level of statistical power allowing for the detection of effects of 
interest in the present study. However, since acute nicotine-withdrawal effects may increase head movement and 
given the potential for unforeseeable errors during data collection, an estimated 20% of the participants who 
complete all phases of the study will likely be removed from the final analyses. Thus, a total of 42 non-treatment 
seeking smokers is requested.   
 
12. Human Subjects Protection 
12.1 Subject selection 
Participant recruitment will be based strictly on the inclusion/exclusion criteria.  No preferences in 
participant recruitment will be made on the bases of gender, race, or ethnic background.  Efforts will be made to 
avoid participant distribution bias such that if skewing is noted, subjects in the over-represented group may 
temporarily be excluded from the study until additional participates from under-represented groups can be 
established.   
Efforts will be made to include ethnic minorities in proportion to their presence in the major metropolitan 
Baltimore 
area 
(Baltimore 
City, 
Anne 
Arundel, 
and 
Baltimore 
and 
Howard 
counties; 
http://quickfacts.census.gov/qfd/states/24/24510.html). 
 
Treatment seeking smokers 
 
APPROVED STUDY POPULATION 
 
FEMALE 
ENROLLMENT 
MALE 
ENROLLMENT 
TOTAL 
ENROLLMENT 
FEMALE 
COMPLETERS 
MALE 
COMPLETERS 
TOTAL 
COMPLETERS 
APPROVED 
CEILING 
61 
59 
120 
43 
42 
85 
 
NIH TARGETED/PLANNED ENROLLMENT 
 
ETHNIC CATEGORY 
Sex/Gender 
Females 
Males 
Total 
Hispanic or Latino 
3 
2 
5** 
Not Hispanic or Latino 
58 
57 
115 
Ethnic Category: Total of All Subjects* 
61 
59 
120* 
RACIAL CATEGORIES 
 
 
 
American Indian/Alaska Native 
0 
0 
0 
Asian 
2 
2 
4 
Native Hawaiian or Other Pacific Islander 
0 
0 
0 
Black or African American 
39 
38 
77 
White 
20 
19 
39 
Racial Categories: Total of All Subjects* 
61 
59 
120* 
*The “Ethnic Category: Total of All Subjects” must be equal to the “Racial Categories: total of All Subjects.” 
 
Non-treatment seeking smokers 
 
APPROVED STUDY POPULATION 
 
FEMALE 
ENROLLMENT 
MALE 
ENROLLMENT 
TOTAL 
ENROLLMENT 
FEMALE 
COMPLETERS 
MALE 
COMPLETERS 
TOTAL 
COMPLETERS 
APPROVED 
CEILING 
21 
21 
42 
18 
17 
35 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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NIH TARGETED/PLANNED ENROLLMENT 
 
ETHNIC CATEGORY 
Sex/Gender 
 
Females 
Males 
Total 
Hispanic or Latino 
1 
1 
2** 
Not Hispanic or Latino 
20 
20 
40 
Ethnic Category: Total of All Subjects* 
 
 
42* 
RACIAL CATEGORIES 
 
 
 
American Indian/Alaska Native 
0 
0 
0 
Asian 
1 
0 
1 
Native Hawaiian or Other Pacific Islander 
0 
0 
0 
Black or African American 
13 
13 
26 
White 
7 
8 
15 
Racial Categories: Total of All Subjects* 
21 
21 
42* 
*The “Ethnic Category: Total of All Subjects” must be equal to the “Racial Categories: total of All Subjects.” 
 
 
 
Older individuals, defined as those over 55, will be excluded from the present study.  Justification: Many 
cognitive processes change with age. In addition, the risk of difficult-to-detect medical abnormalities such as 
silent cerebral infarcts increases with age. 
 
Left-handed individuals will also be excluded.  Justification: Some of the neural processes assessed in this 
protocol may be lateralized in the brain.  In order to reduce potential variance, participants will be required to be 
right-handed.  
 
12.2 
Justification for exclusion of children 
Children will not be included in the present study. This study focuses on the effects of acute nicotine 
withdrawal processes. Given that nicotine is a controlled substance (must be 18 or older to purchase nicotine 
containing products) children under the age of 18 will not be recruited.   
12.3 
Justification for exclusion of vulnerable populations 
There will be no inclusion of vulnerable populations in the current Study. The study focuses on the effects 
of acute nicotine withdrawal on a diverse range of affective, cognitive, behavioral and neurobiological indices. 
To control for variance in these domains we need to exclude individuals suffering from mental illness, intellectual 
impairment or any other condition that may affect neurological functioning (e.g. HIV).  Pregnant women are also 
explicitly excluded due to the unknown risks that MRI poses to the unborn fetus.  
12.4 
Safeguards for vulnerable populations 
Measures of intellectual functioning, mental health and HIV screening will be conducted under protocol 
06-DA-N415 and used in the current protocol to identify and exclude individuals in vulnerable populations. Urine 
and/or blood serum pregnancy testing will also be employed in the current protocol to screen for pregnancy. 
12.5 
Justification of sensitive procedures.  
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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The use of a placebo condition in the current protocol may be considered a sensitive procedure. A placebo 
condition has been included in the current design to avoid the potential for demand characteristics (i.e., expectancy 
bias) to impact on participant behavior/self-report.  
 
In addition, participants will be required to abstain from smoking for 36 hours prior to each scanning session. As 
outlined previously, the primary purpose of the current protocol is to understand the neurobiological mechanisms 
that underlie acute nicotine withdrawal and drive early relapse in smokers. The affective (e.g., anhedonia, anxiety, 
irritability) and cognitive (e.g., difficulty concentrating) withdrawal symptoms following acute nicotine 
abstinence show an onset of 2 to 12 hrs post-cessation and peak within 2-3 days (J R Hughes et al., 1994). Most 
prior imaging and EEG studies have assessed acute withdrawal effects following overnight abstinence (typically 
8 to12 hrs of smoking deprivation; e.g., Azizian et al., 2010; Beaver et al., 2011; D. M. Cole et al., 2010; Loughead 
et al., 2010; Sweet et al., 2010) and a few, 24 hours abstinence (Kozink et al., 2010a; McClernon et al., 2009b). 
Although abstinence effects on task and resting state BOLD or EEG activity have been observed across most 
studies, behavioral effects appear to be less robust with shorter abstinence periods. By limiting their abstinence 
period to 12 or 24 hours studies may be failing to capture or detect neurobiological changes and behavioral 
phenomena which underlie the later peak in acute nicotine withdrawal effects. In the current protocol, 36 hrs was 
selected as an abstinence period that would approximate peak acute withdrawal effects, producing robust effects 
across MRI, EEG and behavioral measures, whilst also maximizing non-smoking compliance. 
 
The administration of varenicline may be considered a sensitive procedure. Varenicline is an FDA approved first-
line treatment for smoking cessation which is more effective than current alternative smoking cessation aids 
(Aubin et al., 2008). In the current study, varenicline will be administered to treatment seeking smokers as part 
of a 12-week treatment for smoking cessation and will therefore be of direct benefit to participants. Employing 
Varenicline with counseling will also reduce relapse rates at 1, 6 and 12 months relative to alternative treatments 
and thus increase our statistical power to be able to examine differences between those individuals who relapse 
and those who remain abstinent at each of these time points. Moreover, varenicline is reasonably well tolerated, 
has an acceptable safety profile, and is associated with low abuse liability (Glover and Rath, 2007). Notably, 
varenicline was associated with significantly greater abstinence rates than NRT at 4 weeks (55.9% vs 43.2%) and 
52 weeks (26.1% vs 20.3%). Varenicline was also associated with significantly less craving, withdrawal 
symptoms and smoking satisfaction compared with NRT (Aubin et al., 2001). 
12.6 
Staff Participation 
 
NIDA-IRP staff will be allowed to participate in this protocol.  Recruitment of staff will be via IRB-
approved announcements and advertisements to the IRP as a whole. No staff member will be directly approached 
to participate in an experiment. To ensure against issues of breach of confidentiality or coercion, only staff from 
outside the Neuroimaging Research Branch and who are not under the supervision of any study investigator listed 
on this protocol will be allowed to participate. Additionally, employee participation will be limited to those doing 
so on their own time so that the incentive tasks will not be undermined by the lack of a payment incentive. No 
personal identifiers will be tagged to any of the data - behavioral, physiological or neurobiological. During the 
screening and recruitment process, non-NIDA participants will be explicitly asked for permission to re-contact 
them in the future. Appropriate methods for such contact (e.g. phone, email, letter) will be determined at the time 
of consent. 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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13. Anticipated Benefit 
 
Treatment-seeking smokers 
Direct benefits: Varenicline administration and smoking-cessation counseling (or alternative 
treatment plans described above) should help decrease participants’ smoking and withdrawal 
discomfort. They may also benefit more generally by receiving psychological assistance in developing 
skills to enable them to cope with stress and other situations which precipitate cigarette smoking. 
 
Non-treatment-seeking smokers 
 
There are no direct benefits to participants in the non-treatment seeking arm of this protocol. The findings 
may benefit future populations by extending understanding of the neurobiological mechanisms underlying 
motivation to quit and the interaction with mechanisms underlying symptoms experienced during acute 
withdrawal from nicotine.  
 
14. Consent documents/Process 
Written informed consent will be obtained from each subject at entry into the study.  The consent 
document will include details regarding all study procedures (e.g. fMRI, EEG, and genotyping). Informed consent 
is obtained by the following process: Subject reviews the study consent form; PI or co-investigator then meets 
with the subject in a private area to review the consent, confirm subject understanding, and to answer any 
questions.  This process will include reading through the document with the participant in order to further ensure 
participant understanding. Once the subject verbally demonstrates understanding to the investigator and agrees to 
the process, a consent quiz is administered. Provided the participant answers at least 80% of the questions 
correctly (which must include correct answers to questions #5 and #9), the participant is invited to sign the consent 
form. If the score on the consent quiz is less than 80% correct, the investigator reviews the incorrect answers and 
re-administers the consent quiz.  Failure to obtain 80% correct (including correct answers to questions #5 and #9) 
on the second administration of the quiz excludes the subject from participating in the study. If the participant 
does score ≥ 80% on the consent quiz, they are asked to sign and date the consent form.  The investigator co-signs 
the consent form and, if necessary, the consent is also signed by a third-party witness to the subject’s signature.  
Two copies are made of the consent. The original copy of the consent and quiz are  attached to the participant’s 
medical record, the participant is given a copy of the consent form for his/her own records and the second copy 
of the consent and a copy of the quiz are stored with other protocol consents in a binder which is located in a 
locked filing cabinet. Once the signed consent has been obtained, the investigator will note the participant’s 
enrollment in the study in the CDW database.  Only after this last step has been completed will study procedures 
begin.   
15. Data and Safety monitoring 
a. Selection of a Data and Safety Monitoring Mechanism  - Data and safety will be monitored by the 
NIDA Principal Investigator and MAI. Subjects will be monitored during the study for any adverse 
reactions and the overall protocol will be monitored by reviewing the AE’s on a yearly basis. The NIDA 
PI will report all adverse events according to established NIH IRB guidelines. This protocol involves no 
more than minimal risk to participants.  

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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b. Frequency of the Monitoring - Subjects will be monitored during the study for any adverse reactions 
and the overall protocol will be monitored by reviewing the AE’s on a yearly basis. 
c. Stop or Change Rules - An unexpected serious adverse event related to study procedures or the 
revelation of information or data not available at the time of study preparation which renders the 
risk/benefit ratio for this study unfavorable will result in study cessation. 
d. Advanced Plans for Any Interim Analyses and/or Futility Analyses - There are no current plans for 
interim analyses and/or futility analyses.  
e. Information To Be Monitored   
a. Progress of the study, including assessment of participant recruitment and accrual and adverse 
events will be reviewed to determine whether there is any change to the risk/benefit ratio of the 
study. This information will be reported to the IRB at the time of continuing review. 
b. Subjects will be monitored for adverse events by study staff.  Adverse events will be recorded by 
staff in the subjects’ medical record and reported to the Clinical Director and NIH IRB in 
accordance with all NIH and NIDA requirements for adverse event reporting. 
f. There are no external factors (e.g., developments in the literature, results of related studies, etc.) that 
may have an impact on the safety of participants or on the ethics of the research study. 
g. Communication - Adverse events will be recorded by staff in the subjects’ medical record and reported 
to the Clinical Director and NIH IRB in accordance with all NIH and NIDA requirements for adverse 
event reporting. Additionally, a summary of study progress, including assessment of participant 
recruitment and accrual and adverse events will be reported to the IRB at the time of continuing review. 
 
15.1. Criteria for Study Cessation 
For the Study as a Whole:  A particular arm of the study will be suspended by the PI, pending CD review, 
if any subject has a serious adverse event that is probably or definitely related to study procedures.   
16. Quality Assurance 
Quality-assurance monitor:  The Principal Investigator and the MAI will be monitoring data collection 
and the study on an ongoing basis.  Quality assurance will monitored by the NIDA Quality-Assurance Team on 
a schedule determined by the Clinical Director. 
Quality-assurance plan: We will use the quality-assurance plan that is being developed by the NIDA IRP 
Clinical Director.   
17. Event Characterization and reporting to the IRB, Clinical 
Director (CD) and Sponsor 
 
NIH OHSRP Policies 801 and 802 (effective July 1, 2019; supersedes SOP 16) will be followed for 
reportable events and non-compliance. 
 
18. Alternatives to participation or alternative therapies 
Treatment-seeking smokers who are not eligible to participate in this protocol or who no longer want to 
participate in this protocol may be able to receive smoking cessation treatment outside of the study, under the 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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care of their own physician. They will be given referrals, when appropriate, for smoking-cessation help in the 
community. 
Non-treatment seeking smokers who are not eligible to participate in this protocol or who longer wish to 
participate in this protocol and who express a desire at this point to seek treatment for smoking cessation will be 
given referrals to smoking cessation treatment outside of the study, as described above for treatment-seeking 
smokers. 
Nonsmokers (enrolled in tDCS arm) who are not eligible to participate in this protocol, or who no longer 
wish to participate in this protocol, may choose not to join the study or to withdraw at any time. 
19. Confidentiality 
Strict subject confidentiality will be maintained throughout. Participants will be assigned a code number 
without personally-identifying information following their first contact in the protocol. This number will be used 
throughout the experiment and will be the only identifier on specimen samples, behavioral and physiological 
archival data, fMRI/MRI and EEG/ERP data.  The identity of participants will not be revealed at scientific 
meetings, in publications or other vehicles of public communication. The PI and co-investigators will have access 
to the ID code, which will be maintained in a separate electronic file from the study data. Medical and 
questionnaire data will be gathered in the CDW, a secure database on a closed network. Access to records in the 
CDW is protected by a system of password-protected accounts and monitored by the Clinical Director (CD). Data 
downloaded from the CDW for analysis will be identified only by participant number. 
19.1. Medical Records.   
All medical history information is stored in the CDW database, which is password protected and has 
limited access.  In addition, each participant is assigned a medical records folder during the screening process, 
which is used to store paper copies of medically relevant documents.  This record is kept in locked cabinet and 
access to these files is limited to study personnel, including study investigators, nursing staff and clinicians.    
19.2. Research Records/Data.  
 Paper copies of research records such as PI copies of consents and ‘Run Sheets’ (procedural checklists) 
used by investigators will be kept in binders. These binders will be kept in a locked cabinet, in a locked room, 
which has limited access.  Binders remain in this room at all times, apart from when required for study sessions.  
At the completion of each paper copies of research records or binders will be returned to the locked cabinet by 
the investigator or research associate responsible for the experimental session. Data (physiological, imaging, 
behavioral) obtained during experimental sessions is stored on password protected, network drives, which have 
limited access.  Data stored on these drives is identified by study number, participant ARC and/or task.  No 
personal identifiers are stored with the data.  
19.3. Stored Samples.  
No biological samples will be stored for future use. Plasma samples acquired for measurement of cortisol and 
ACTH will be destroyed once valid assays have been obtained. 
19.4. Special Precautions.  
A Certificate of Confidentiality will be obtained for this protocol and strict subject confidentiality will be 
maintained throughout. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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20. Conflict of Interest/Technology Transfer 
NIH guidelines on conflict of interest have been distributed to all investigators.  There are no conflicts-
of-interest to report. 
A human Material Transfer Agreement (MTA) between NIDA-IRP and the Board of Supervisors of 
Louisiana State University and Agricultural and Mechanical College acting on behalf of LSU Health Sciences 
Center at Shreveport for the analysis of plasma samples has been obtained. The Recipient Scientist at LSU, Hyung 
Nam, Ph.D., will receive coded samples to conduct analysis of plasma neuro-steroids and amino acids to compare 
with nicotine satiety, the effect of acute nicotine withdrawal on plasma neuro-steroids and amino acid profiles 
and their relationship with measured glutamate concentration (MRS) in the cingulate cortex. 
21.  Research and Travel Compensation 
Treatment seeking smokers 
 
All participants will be compensated for their participation in the pre-treatment scan and orientation visits 
and the three follow-up visits and related procedures according to NIDA-IRP policy summarized in Table 1. 
Compensation will be prorated for parts completed if subjects do not complete the study.  Participants will also 
earn points on several tasks/procedures which will be exchanged at the end of each scanning session for additional 
monetary payment (as outlined). They will also receive incentives for successful abstinence during the pre-
treatment study phase. At the end of the second pre-treatment scan day they will also receive a 10% completion 
bonus based on payment for study related procedures and MRI scanning. This completion bonus is only based on 
the hours participated during Phase 1, not on any other compensation received during Phase 1. For example, 28 
hours of Phase 1 procedures @ $20 per hour = $560, so 10% of this is $56. Participants will have the option of 
cash or check payment. If participation runs beyond 6:30 pm or occurs on weekends, payment by check may be 
the only option. Participants will receive payment earned for each day at the end of each day of participation. In 
the rare instances where a check is pre-issued because procedures are scheduled after 6:30 or on weekends, the 
amount due the participant will be estimated on the low end, and the participant will receive any balance due 
either at their next session, or via a check in the mail (whichever they prefer). The minimum amount of time 
required for study procedures per scan visit is 8 hours (with 1 hour lunch break). We have based maximum 
payment on 10 hours of study related procedures per visit to allow for additional time when procedures take 
longer than expected. Participants will receive travel compensation for all Phase 1, 2 and 3 visits (up to 21 visits). 
Payment for at home procedures will be contingent upon maintaining abstinence for the full 36 hour period. 
 
 
Rate 
Time/frequency  
Total 
Study related procedures incl. MRI 
scanning 
 
$20/hr 
32hrs 
$640 
At home procedures 
$20 per period  
2 periods 
$40 
Performance incentives 
$25 day 
2 days 
$50 
Task payment 
Up to $70 per day 
2 days 
$140 
Indwelling catheter  
$10/day 
2 days 
$20 
Travel 
$15 per round trip 
21 trips 
$315 
Completion bonus 
10% total 
1 
$56.00 
 
Max Total 
 
 
 
$1261.00 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Non-treatment seeking smokers 
 
All participants will be compensated for their participation in the pre-treatment scan and orientation visits 
related procedures according to NIDA-IRP policy summarized in Table 1. Compensation will be prorated for 
parts completed if subjects do not complete the study.  Participants will also earn points on several 
tasks/procedures which will be exchanged at the end of each scanning session for additional monetary payment 
(as outlined). They will also receive incentives for successful abstinence during the pre-treatment study phase. At 
the end of the second pre-treatment scan day they will also receive a 10% completion bonus based on payment 
for study related procedures and MRI scanning. This completion bonus is only based on the hours participated 
during Phase 1, not on any other compensation received during Phase 1. For example, 28 hours of Phase 1 
procedures @ $20 per hour = $560, so 10% of this is $56. Participants will have the option of cash or check 
payment. If participation runs beyond 6:30 pm or occurs on weekends, payment by check may be the only option. 
Participants will receive payment earned for each day at the end of each day of participation. In the rare instances 
where a check is pre-issued because procedures are scheduled after 6:30 or on weekends, the amount due the 
participant will be estimated on the low end, and the participant will receive any balance due either at their next 
session, or via a check in the mail (whichever they prefer). The minimum amount of time required for study 
procedures per scan visit is 8 hours (with 1 hour lunch break). We have based maximum payment on 10 hours of 
study related procedures per visit to allow for additional time when procedures take longer than expected. 
Participants will receive travel compensation for all Phase 1 visits. Payment for at home procedures will be 
contingent upon maintaining abstinence for the full 36 hour period. 
 
 
 
Rate 
Time/frequency  
Total 
Study related procedures incl. MRI 
scanning 
 
$20/hr 
28hrs 
$560 
At home procedures 
$20 per period  
2 periods 
$40 
Performance incentives 
$25 day 
2 days 
$50 
Task payment 
Up to $70 per day 
2 days 
$140 
Indwelling catheter  
$10/day 
2 days 
$20 
Travel 
$15 per round trip 
3 
$45 
Completion bonus 
10% total 
1 
$56.00 
 
Max Total 
 
 
 
$911.00 
 
22. Motivational Interviewing Arm 
22.1 Arm Title 
474 – Motivational interviewing to increase engagement in smoking cessation treatment. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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22.2 Arm Background  
The 2010 National Health Interview survey (n = 27, 157) reported that 69% of current smokers indicate 
a desire to stop smoking completely (Malarcher, Dube, Shaw, Babb, & Kaufmann, 2011). In contrast, the 
prevalence of successful smoking cessation (> 6 months without smoking) within the past 12 months fell at 6% 
(Malarcher et al., 2011).  One of the primary factors driving the discrepancy between self-reported interest in 
quitting and successful cessation is the degree of motivation and preparation to quit smoking and maintain long-
term abstinence (Center for Substance Abuse Treatment, 1999). Thus while smokers may express a desire to 
quit, this is often coupled with a reluctance to set a quit date and commit to long-term smoking abstinence. 
Motivational interviewing is a therapeutic technique designed to directly address this ambivalence and increase 
engagement in substance abuse treatment (Center for Substance Abuse Treatment, 1999).  
22.3. Arm Objective  
The purpose of the Motivational Interviewing Arm is to increase motivation and preparation to for 
smoking cessation treatment among individuals who express an interest in quitting smoking but are not currently 
ready to enter smoking cessation treatment, and are otherwise eligible for participation in the current protocol 12-
DA-N474 or protocol 13-DA-N485.  If, after a prescribed period of motivational interviewing, the participant and 
the study therapist feel that the participant is ready to enter treatment for smoking cessation, they will be offered 
admission into either protocol 12-DA-N474 or protocol 13-DA-N485. 
22.4 Number and type of subjects  
This study arm will recruit 300 current smokers who meet criteria for participation in protocol 12-DA-
N474 (see section 3 Subjects) or protocol 13-DA-N485) but who are not currently ready to enter treatment for 
smoking cessation.  
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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APPROVED STUDY POPULATION 
 
FEMALE 
ENROLLMENT 
MALE 
ENROLLMENT 
TOTAL 
ENROLLMENT 
FEMALE 
COMPLETERS 
MALE 
COMPLETERS 
TOTAL 
COMPLETERS 
APPROVED 
CEILING 
150 
150 
300 
150 
150 
300 
NIH TARGETED/PLANNED ENROLLMENT 
 
ETHNIC CATEGORY 
Sex/Gender 
 
Females 
Males 
Total 
Hispanic or Latino 
6 
6 
12** 
Not Hispanic or Latino 
144 
144 
288 
Ethnic Category: Total of All Subjects* 
 
 
300* 
RACIAL CATEGORIES 
 
 
 
American Indian/Alaska Native 
1 
1 
2 
Asian 
3 
3 
6 
Native Hawaiian or Other Pacific Islander 
0 
0 
0 
Black or African American 
99 
99 
198 
White 
47 
47 
94 
Racial Categories: Total of All Subjects* 
150 
150 
300* 
*The “Ethnic Category: Total of All Subjects” must be equal to the “Racial Categories: total of All Subjects.” 
 
23.5. Arm design and procedures  
An initial session will be conducted to assess individual motivation/preparation to quit. During this 
session participants will complete the treatment preparation and motivation to quit measures outlined in Appendix 
9 and meet briefly with a study therapist (~60min). This initial session will be followed by 1 to 10 sessions of 
motivational interviewing with a study therapist (each session ~20-60min). Motivational interviewing techniques 
are based on the SAMHSA Treatment Improvement Protocol (TIP) for enhancing motivation for substance abuse 
treatment (Center for Substance Abuse Treatment, 1999). Individuals recruited for this study will vary in their 
motivation and preparation to quit. Consequently, the number of motivational interviewing sessions will vary 
according to individual need as determined by the study therapist, with a maximum of 10 sessions per individual. 
23.6 Storage of data 
Clinical data will be stored in the Clinical Data Warehouse (CDW) and in clinical research charts.  Research 
charts will be stored in locked filing cabinet, in a locked room at the NIDA-IRP.  One copy of the consent form 
is kept in the participant’s NIDA-IRP medical chart, the other is kept in a limited access, locked cabinet with 
other consent forms from the same protocol. Participant data contained within files on the NRB data drive are 
identified by ARC number, and not by other personal identifiers such as name.   
23.7 Risks/discomforts 
Questionnaire and Characterization Measures 
 
The questions asked may be perceived as embarrassing or may cause anxiety or distress.  Nevertheless, such 
reactions are expected to be temporary and to rapidly dissipate spontaneously.  
 
Risk Minimization.  Participants will be advised prior to completing these measures that they may refuse to answer 
any questions that cause them discomfort.  Furthermore, any participant who experiences anxiety or distress as a 
result of these assessments will be able to speak to a NIDA-IRP mental health professional to discuss this.   
 

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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23.8 Outcome measures/Statistical Analysis 
 
There will be no outcome measures or collation and analysis of data at a group-level in this arm of the study.  
 
23.9. Human subjects protection  
 
The same as outlined for above in Section 12. Human Subjects Protection 
 
23.10. Anticipated benefit  
 
Direct benefits: motivational interviewing should help increase participant’s motivation to participate in smoking 
cessation treatment, which should in turn decrease their smoking behavior and withdrawal discomfort.  
 
 
23.11 Consent documents/process, Data and safety Monitoring, Quality Assurance, Adverse event and 
unanticipated problem reporting, Alternatives to participation, Confidentiality, Conflict of Interest 
 
Details for the above sections are identical to those outlined within Sections 14-19 of the main protocol document.  
23.12 Research and Travel Compensation 
Participants will be reimbursed $20 per hour for the initial assessment visit but will not receive payment 
for the motivational interviewing visits. They will also receive $15 travel compensation for each visit.  Based on 
motivational counseling visits and a single pre-assessment visit, anticipated maximum compensation for 
participation in the Motivational Interviewing Arm of this study is $185.  
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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23. Transcranial Direct Current Stimulation (tDCS) Arm  
24.1. Arm Title 
474 – Transcranial Direct Current Stimulation (tDCS) to reduce effects of the Nicotine Withdrawal Syndrome 
24.2. Arm Background 
Cigarette smoking remains the leading cause of preventable death and disease in the United States, accounting 
for 480,000 deaths each year (CDC 2016). Despite our deeper understanding of the neurobiology of nicotine 
addiction and drug abuse over the past 10 years (Nestler 2005, Everitt and Robbins 2005, Koob and Volkow 2010, 
Goldstein and Volkow 2011), current pharmacological interventions for smoking cessation are highly inadequate, 
with the most efficacious treatment (varenicline) having only about 27% absolute cessation rate according to 
recent meta-analysis (Cahill 2013, 2014). Additionally, symptoms of nicotine withdrawal remain a major 
impediment for smokers trying to quit, with most quit attempts failing within the first week of abstinence when 
withdrawal symptoms are at their height (Hughes 1992, Sutherland et al 2017). 
 
Recent findings based on resting state functional connectivity (rsFC) revealed that the brain can be divided into 
multiple large-scale functional networks, many of which have been found to contribute to psychiatric disease (Lu 
and Stein 2014, Turk-Browne 2013, Yeo et al 2011, Fox et al 2014). Addiction has been hypothesized as an 
imbalance within and between three of these networks (Lerman et al 2014, Pariyadath et al 2015, Fedota and 
Stein 2015, Sutherland et al 2012). Specifically, the cognitive and affective disturbances of nicotine withdrawal 
syndrome have been attributed to a reduced within-network connectivity of the Executive Control Network (ECN) 
and increased within-network connectivity of the Default Mode Network (Sutherland et al 2017). Additionally, 
the Salience Network (SN) has been called the “gatekeeper” for these two networks, and its function may be 
dysregulated in addictive disorders, as well (Sutherland et al 2017). 
 
A treatment that acts by modifying these large-scale brain networks with specificity may help address the 
underlying pathophysiology of nicotine addiction and improve clinical outcomes (Dunlop et al 2016). 
Transcranial Direct Current Stimulation (tDCS), a type of Non-invasive Brain Stimulation (NIBS), has the 
potential to modify neuronal circuits by application of a subthreshold conductive current through the scalp. Two 
potential targets for tDCS as a smoking cessation aid are the dorsolateral pre-frontal cortex (dlPFC), a node of 
the ECN, and the ventromedial prefrontal cortex (vmPFC), a node of the DMN (Lerman et al 2014). tDCS can 
potentially strengthen the control of the ECN through excitatory stimulation of the dlPFC, and weaken the 
influence of the DMN by inhibitory stimulation of the vmPFC. 
24.3. Arm Objective 
The goal of the Transcranial Direct Current Stimulation (tDCS) Arm is to investigate the effects of tDCS on 
modulating large-scale brain networks dysregulated in nicotine addiction and withdrawal. Non-smokers and 
matched smokers (in both nicotine deprived and sated conditions) will be recruited for a randomized, sham-
controlled, double-blind, repeated measures tDCS crossover study. tDCS will be used to probe the ECN and DMN 
by targeting the dlPFC with anodal (excitatory) stimulation, and the vmPFC with cathodal (inhibitory) stimulation 
(Pariyadath et al 2015). The effects of tDCS on brain activation patterns and cognitive control will be measured 
“online” while the subject is being scanned via MRI, allowing greater insight into the neurophysiological effects 
of tDCS.  
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Arm Hypothesis:  The nicotine withdrawal syndrome is a 3-network dysregulation characterized by reduced 
network connectivity (1) within the ECN and (2) between the ECN and the SN, as well as increased network 
connectivity (1) within the DMN, and (2) between the DMN and the SN. These deficits can be remediated by 
application of tDCS to cortical nodes of the ECN and DMN networks. (Figure 1a). 
 
 
Figure 1a: Hypothesis. Three network dysregulation in nicotine addiction and hypothesized effects of tDCS. The ECN 
and DMN are anticorrelated. In nicotine withdrawal syndrome, the within-network connectivity of the DMN is stronger than 
normal, and the within-network connectivity of the ECN is weaker than normal. This imbalance is mediated by dysregulated 
connectivity between the SN - DMN and the SN - ECN. SN = Salience Network. ECN = Executive Control Network. DMN 
= Default Mode Network. dlPFC = dorsolateral prefrontral cortex. vmPFC = ventromedial prefrontal cortex.  Image 
adapted from Lerman et al 2014. 
 
Alternative Hypothesis (1): Nicotine withdrawal syndrome is a 2-network dysregulation characterized by 
altered homeostatic regulation between the ECN and DMN. It is possible that the ECN normally acts to 
weaken the DMN, while the DMN normally acts to weaken the ECN. In the nicotine withdrawal syndrome, 
the ECN may have weakened control over the DMN, leading to a stronger within-network connectivity of the 
DMN and weaker within-network connectivity of the ECN. Anodal tDCS applied to the dlPFC can return the 
2 networks to homeostatic regulation by strengthening the within-network connectivity of the ECN, while 
cathodal tDCS applied to the vmPFC can return the 2 networks to homeostatic regulation by weakening the 
within-network connectivity of the DMN. (Figure 1b). 
 
 
 
Figure 1b: Alternative hypothesis. Two network dysregulation in nicotine addiction and hypothesized effects of 
tDCS. SN = Salience Network. ECN = Executive Control Network. DMN = Default Mode Network. dlPFC = 
dorsolateral prefrontral cortex. vmPFC = ventromedial prefrontal cortex. Image adapted from Lerman et al 2014. 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Alternative Hypothesis (2): It is also possible that the imbalances seen in nicotine addiction and withdrawal 
are due to an abnormal connectivity of only one of the two networks (DMN or ECN), either internally within 
the network or in connection with the Salience Network. The within-network connectivity of the DMN, or its 
between-network connectivity with the SN, may be stronger independently of changes to the ECN. 
Alternatively, the within-network connectivity of the ECN, or its between-network connectivity with the SN, 
may be weaker independently of changes to the DMN. The exact relationship may differ across individuals. 
In either case, anodal-dlPFC + cathodal-vmPFC tDCS is expected to bring the within and between network 
connectivities of the DMN and the ECN closer to the healthy control state. 
 
Specific Aims 
 
Aim 1: To determine the effect of excitatory tDCS stimulation of the dlPFC and inhibitory tDCS stimulation 
of the vmPFC on the within-network and between-network connectivity of the Executive Control Network 
(ECN), Default Mode Network (DMN), and Salience Network (SN). 
 
To understand the effect of anodal stimulation of the dlPFC, and cathodal stimulation of the vmPFC, we will compare 
the following tDCS montages: (1) anodal left-dlPFC + cathodal right-vmPFC stimulation, with anode over the left 
dlPFC and cathode over the right-vmPFC; (2) cathodal left-dlPFC + anodal right-vmPFC stimulation, in which 
polarity is reversed between the two electrodes, and (3) sham stimulation..  
 
Sub-Aim 1.1: To determine the effect of tDCS stimulation on the Executive Control Network (ECN) and 
cognitive control during nicotine withdrawal. Resting state connectivity, behavioral tasks and functional 
imaging will be used to measure the strength of the ECN and cognitive control mechanisms in response to 
excitatory tDCS stimulation of the left dlPFC, a cortical node of the ECN. 
 
Hypothesis 1.1.1. Anodal-dlPFC tDCS will strengthen the within-network connectivity of the Executive 
Control Network compared to sham as measured by resting state functional connectivity in abstinent smokers 
> sated smokers > healthy controls. Cathodal-dlPFC tDCS will have the inverse effect on ECN rsFC. 
 
Hypothesis 1.1.2. Anodal-dlPFC tDCS will improve reaction time and response accuracy on cognitive 
control tasks (Parametric Flanker, Amygdala Reactivity Task, and N- back) in abstinent smokers > sated 
smokers > healthy controls. The improved behavioral performance will be correlated with increased BOLD 
signal in nodes of the ECN. Cathodal-dlPFC tDCS will have the inverse effect on BOLD signal and behavior. 
 
Sub-Aim 1.2: To determine the effect of tDCS stimulation on the Default Mode Network (DMN) during 
nicotine withdrawal. Resting state connectivity, behavioral tasks and functional imaging, magnetic resonance 
spectroscopy, and white matter integrity will be used to measure the strength of the DMN and cognitive control 
mechanisms in response to excitatory tDCS stimulation of the left dlPFC, a cortical node of the ECN, and 
inhibitory tDCS stimulation of the left vmPFC, a cortical node of the DMN. 
 
Hypothesis 1.2.1. Cathodal-vmPFC tDCS will weaken the within-network connectivity of the Default Mode 
Network as measured by resting state functional connectivity in abstinent smokers > sated smokers > healthy 
controls. Anodal-vmPFC tDCS will have the inverse effect on DMN rsFC. 
 
Hypothesis 1.2.2. Cathodal-vmPFC tDCS will improve reaction time and response accuracy on cognitive 
control tasks (Parametric Flanker, Amygdala Reactivity Task, and N-back) in abstinent smokers > sated 

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smokers > healthy controls. The improved behavioral performance will be correlated with greater BOLD 
deactivation in nodes of the DMN during these tasks. Anodal-vmPFC tDCS will have the inverse effect on 
BOLD signal. 
 
Sub-Aim 1.3: To determine the effect of tDCS stimulation on the Salience Network (SN) and its connectivity 
with the ECN and the DMN. 
 
Hypothesis 1.3.1. Anodal-dlPFC tDCS will strengthen the between-network connectivity of the Executive 
Control Network to the Salience Network compared to sham as measured by resting state functional 
connectivity in abstinent smokers > sated smokers > healthy controls. Cathodal-dlPFC tDCS will have the 
inverse effect on ECN-SN rsFC. 
 
Hypothesis 1.3.2. Cathodal-vmPFC tDCS will weaken the between-network connectivity of the Default 
Mode Network to the Salience Network as measured by resting state functional connectivity in abstinent 
smokers > sated smokers > healthy controls. Anodal-vmPFC tDCS will have the inverse effect on DMN-SN 
rsFC. 
 
Hypothesis 1.3.3. Anodal-dlPFC + Cathodal-vmPFC tDCS will improve BOLD reactivity in nodes of the 
Salience Network in tasks that are known to those nodes (e.g. the insula in the Parametric Flanker Task). 
Cathodal-dlPFC + Anodal-vmPFC will have the inverse effect on BOLD reactivity in the SN. 
 
Aim 2: To compare the effects of tDCS stimulation parameters on behavior, resting state functional 
connectivity, and BOLD fMRI signal. 
 
Sub-Aim 2.1: To compare the effect of tDCS stimulation on a working memory task given during 
stimulation (online) and after stimulation (offline).  
 
Hypothesis 2.1.1: Active tDCS stimulation will provide greater benefit on accuracy and response time, 
and increase BOLD activation in the ECN to a greater degree, during the “online” working memory task 
than during the “offline” working memory task. 
 
Alternative Hypothesis 2.1.1(a): Active tDCS stimulation will provide greater the same degree of 
benefit on accuracy and response time, and increase BOLD activation in the ECN to the same degree, 
during the “online” working memory task as during the “offline” working memory task. 
 
Alternative Hypothesis 2.1.1(b): Active tDCS stimulation will provide less benefit on accuracy and 
response time, and increase BOLD activation in the ECN to a smaller degree, during the “online” 
working memory task than during the “offline” working memory task. 
 
 
 

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Aim 3: To compare the effects of tDCS stimulation on nonsmokers vs. smokers, in both the nicotine sated 
and nicotine deprived states. 
 
Hypothesis 3.1. Active tDCS will have the greatest effect on the group farthest from normal resting 
functional connectivity at pre-stimulation baseline, i.e. smokers in withdrawal. The non-smoker group 
will respond the least to active tDCS. Smokers using a nicotine patch will respond to tDCS more than 
non-smokers, but not as much as smokers in withdrawal. Thus, tDCS will have the greatest impact on 
network connectivity and behavior in the smoker-nicotine withdrawal group, as measured by outcomes 
listed in Aim 1. 
 
Aim 4: To evaluate the effect of individual variability on current localization, behavioral outcomes, and 
brain activation patterns in response to active tDCS stimulation.  
 
Hypothesis 4.1. Anatomical Variability. As each individual has variation in head and brain size, skull 
thickness, and brain morphometry, it is expected that a standard tDCS application across all individuals 
will produce varied effects in each individual participant. Computer modeling of each participant’s brain 
can approximate how standard tDCS stimulation parameters will affect each individual based on their 
own anatomy. We will use a post-hoc regression analysis to determine how outcome measures correlate 
with inter-individual anatomical variability. 
 
Individuals who are predicted to have greater current flow through the dlPFC and vmPFC by computer 
modeling will also have greater responsivity to tDCS stimulation, as measured by outcomes listed in Aim 
1. 
 
Hypothesis 4.2. Psychological Variability. Cigarette smoking behavior is usually accompanied by a 
variety of co-morbid conditions and traits, such as depressed mood, increased anxiety, and use of 
substances such as marijuana and alcohol. Although participants will be excluded for serious co-
morbidities, including moderate to severe mood or anxiety disorders, or substance use disorders (other 
than nicotine), the traits of these disorders fall on continuous scales that show variance among individuals 
before the diagnostic threshold. These individual differences can affect performance on the fMRI tasks, 
resting state functional connectivity, and response to tDCS.  
 
We will use a post-hoc regression analysis to determine how these outcome measures correlate with inter-
individual differences in personality, mood, anxiety, and substance use. It is predicted that individuals 
prone to depressed mood, increased anxiety, and more frequent use of marijuana or alcohol will have 
more abnormal resting connectivity at baseline, and therefore will be more responsive to tDCS 
stimulation, as measured by outcomes listed in Aim 1. 
 
 
 

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smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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24.4. Number and type of subjects 
There will be two study populations: 1) healthy nicotine-dependent adult participants who are current non-
treatment seeking smokers; and 2) healthy non-smoking, non-drug dependent controls. 
 
This study arm will recruit 60 current non-treatment seeking smokers (enrollees) with the expectation of 35 
completers. Additionally, we will recruit 55 non-smokers (enrollees) attempted to match based on recruitment 
availability, by education, age, race, and gender, with the expectation of 45 completers (of either the Behavioral 
Pilot or tDCS Imaging study).  
24.5. Modified Inclusion/Exclusion Criteria for tDCS Study Arm  
The inclusion and exclusion criteria have been modified from the main protocol to best address the Aims of the 
Arm. In general, the spirit of the criteria in the main protocol remain the same. For the reader’s convenience, the 
complete Inclusion/Exclusion criteria for this Arm are found below. Checklist can be found in Appendix 1. 
 
Modified Inclusion Criteria. All Subjects must: 
(1) Be between the ages of 18-65.  
(2) Be right-handed. Assessment tool: Edinburgh Handedness Inventory. 
(3) Be in good health. Justification:  Many illnesses may alter fMRI signals as well as cognitive processes 
and neural functioning.  Assessment tool(s): Participants will provide a brief health history during phone 
screening, and undergo a medical history and physical (H&P) examination with a qualified IRP clinician. 
(4) Be free of current moderate to severe DSM-V Substance Use Disorder on any drug, except nicotine in 
smokers. Those with past moderate to severe use disorder on substances may be included, provided they 
are in sustained remission (and not on maintenance therapy for opioid use disorder) and are not 
intoxicated on the day of the imaging session. Justification: Moderate to severe use disorder on other 
substances may result in unique CNS deficits that could confound results and introduce excessive 
variance, while mild substance use disorder and substance use disorder in remission are common in 
community samples of smokers. Assessment tool(s): Computerized SCID or comparable assessment and 
DSM-5 substance use disorder assessment. 
(5) Be able to abstain from alcohol and other recreational drugs for 24 hours before each imaging session, 
and able to moderate caffeine intake 12 hours before each imaging session. Justification: Recent 
substance use, including alcohol, and caffeine modulate neural functioning in a way that would 
complicate data interpretation. Assessment tool(s): Self-report, breathalyzer, and urine toxicology screen 
with follow up neuromotor assessment to ensure absence of acute impairment with positive urine test.  
 
Smokers must meet the additional criteria (6) and (7): 
(6) Have a urine cotinine level corresponding to smoker status for the specific test being used, typically 
corresponding to a urine cotinine above about 200 ng/ml, and have been smoking for at least 1 year. 
Justification: The present protocol is interested in neurobiological mechanisms that underlie nicotine 
withdrawal, and is thus contingent on the presence of nicotine dependence. Assessment tool(s): Self-
report, commercial urine cotinine test corresponding to smoker status for the specific test being used, 
typically corresponding to a urine cotinine above about 200 ng/ml. 

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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(7) Be able to abstain from smoking for 12 hours prior to MRI-tDCS study sessions. Justification: The 
present protocol will investigate the effect of acute nicotine withdrawal on cognitive processes and 
response to tDCS. Assessment: Self-report and expired CO levels. 
 
In addition, non-smokers must meet criteria (8): 
(8) Not have a history of daily cigarette smoking or have used any nicotine products continuously lasting 
more than a month, and no smoking or continuous use of any nicotine products within the past year. 
Assessment Tools: Self-report, urine cotinine test, and expired CO levels. 
 
Modified Exclusion Criteria. All participants will be excluded if they: 
(1) Are not suitable to undergo an fMRI experiment due to certain implanted devices (cardiac pacemaker or 
neurostimulator, some artificial joints, metal pins, surgical clips or other implanted metal parts), body 
morphology, or claustrophobia. Justification: MR scanning is one of the primary measurement tools used 
in the protocol. Assessment tool(s): Prospective participants will fill out an MRI screening questionnaire 
and undergo an interview with an MR technologist.  Questions concerning suitability for scanning will 
be referred to the MR Medical Safety Officer. Prospective participants will be questioned about 
symptoms of claustrophobia and placed in the mock scanner during their first visit to assess for possible 
difficulty tolerating the confinement of the scanner and for ability to fit into the scanner. 
 
(2) Have musculoskeletal abnormalities restricting an individual’s ability to lie flat for extended periods of 
time. Justification: MR scanning sessions require participants to lie flat on their backs and remain 
perfectly still for approximately one hour. Therefore, conditions that would make that difficult (e.g. 
chronic back pain, significant scoliosis) will be exclusionary. Assessment tool(s): History and physical 
examination by a qualified IRP clinician, supplemented with a trial of lying in the mock scanner to assess 
comfort issues. 
 
(3) Have HIV or Syphilis. Justification: HIV and Syphilis both can have central nervous system (CNS) 
sequelae, thus introducing unnecessary variability into the data. Assessment tool(s): Oral HIV followed 
by blood test if oral test is + and STS+ without adequate prior treatment 
 
(4) Regularly or intermittently use any prescription (e.g., benzodiazepines, barbiturates), over-the-counter 
(e.g., cold medicine) medications that are likely to alter BOLD signal (neuronal-vascular coupling). 
Justification: The use of these substances may alter the fMRI signal and/or neural functions of interest in 
the current study. Assessment tool(s): History and comprehensive urine drug screening to detect 
benzodiazepines, antipsychotics, anticonvulsants, and barbiturates. Note: If a participant is intermittently 
taking a medication likely to affect BOLD signal, the participant may be excluded or if scanned, will be 
scanned in the same medication state for data continuity purposes (i.e. either all scan days are scheduled 
after 5 half-lives since last medication use; or all scan days are scheduled on medication). 
 
 
(5) Have any current neurological illnesses including, but not limited to, seizure disorders, frequent migraines 
or on prophylaxis, multiple sclerosis, cerebrovascular accident, movement disorders (except essential 
tremor, so long as it would not interfere with study tasks such as button pressing), history of significant 
head trauma, or CNS tumor. Justification: Neurological diseases alter CNS function and, possibly, the 
neuronal-vascular coupling that forms the basis of the fMRI signal.  Assessment tool(s):  History and 
physical examination by a qualified IRP clinician, urine drug screening for anticonvulsants not disclosed 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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by history. History of head trauma with loss of consciousness of more than 30 minutes or with post-
concussive sequelae lasting more than two days, regardless of loss of consciousness, will be exclusionary. 
The MAI who will also retain discretion to exclude based on a history of neurological illness that may 
compromise data integrity.  
 
(6) Have  current major psychotic disorders, mania, substance-induced psychiatric disorders, or any current 
suicidal ideations or history of suicide attempts. Moderate to severe current symptoms of mood or anxiety 
disorders will be exclusionary as well. However, mild mood or anxiety disorder symptoms will not be 
exclusionary, whether medicated or unmedicated. The MAI will reserve the right to exclude on the basis 
of psychiatric history not explicitly described in this criterion. Justification: Psychiatric disorders involve 
the central neural system (CNS) and, therefore, can be expected to alter the fMRI measures being used in 
this study, however, some degree of mood and anxiety symptoms are common in community samples of 
smokers. Assessment tool(s): Computerized SCID or comparable assessment, and clinical interview 
confirmation by clinician.  
(7) Are cognitively impaired or learning disabled. Justification: Cognitive impairment and learning 
disabilities may be associated with altered brain functioning in regions recruited during laboratory task 
performance. Cognitive impairment may affect one’s ability to give informed consent. Assessment 
tool(s): History of placement in special-education classes as a consequence of serious learning problems 
and not solely as a consequence of behavioral problems, assessed during the History and Physical 
screening assessment. 
(8) Have significant cardiovascular conditions that would make use of nicotine patch unsafe. Justification: 
Nicotine patch may cause significant arrhythmias in susceptible individuals. Assessment tool(s): History 
and physical exam, including 12-lead EKG. 
 
(9) Have any other major medical condition, such as diabetes mellitus, that in the view of the investigators 
would compromise the safety of an individual during participation, or the quality of data obtainable.  
Justification: Many illnesses not explicitly covered here may increase risk or alter important outcome 
measures. Assessment tool(s): History and physical examination by a qualified IRP clinician and CBC, 
urinalysis, NIDA chemistry panel (liver function tests, electrolytes, kidney function). The following lab 
values will result in exclusion from the study: 
 
i. Hemoglobin < 10 g/dl 
ii. White Blood Cell Count < 2400/µl  
iii. Liver Function Tests > 3X upper limit of normal  
iv. Serum glucose > 200 mg/dl 
v. Urine protein > 2+ 
vi. Serum creatinine > 2 mg/dl  
 
The MAI will retain discretion to exclude based on less extreme lab results.  After the screening process 
has been completed, the MAI will take into account all data collected in order to decide if there is an 
existing medical illness that would compromise participation in this research. 
 
(10) Are pregnant, planning to become pregnant, or breastfeeding.  Females are instructed in the consent to 
use effective forms of birth control during the study period.  Justification: study procedures and drugs 
used in the current protocol may complicate pregnancy or be transferred to nursing children.  Assessment 

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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tool(s): Urine and/or serum pregnancy tests, and clinical interview.  Urine pregnancy tests will be 
conducted at the beginning of each imaging visit. 
 
(11) Are non-English speaking. Justification: There is no direct benefit to participants in this study. To include 
non-English speakers, we would have to translate the consent and other study documents and hire and 
train bilingual staff, which would require resources that we do not have and could not justify given the 
small sample size for each experiment. Additionally, the data integrity of some of the cognitive tasks and 
standardized questionnaires used in this study would be compromised as they have only been validated 
in English. Most importantly, ongoing communication regarding safety procedures is necessary when 
participants are undergoing MRI and tDCS procedures. The inability to effectively communicate MRI 
and tDCS safety procedures in a language other than English could compromise the safety of non-English 
speaking participants. Assessment tool(s): self-report. 
 
 
The following exclusion criteria are new for the tDCS study: 
(12) Participated in any brain stimulation session less than two weeks ago, or underwent brain stimulation 
exposure for treatment purposes in the last 6 months, including tDCS or transcranial magnetic stimulation 
(TMS). Justification:  Prior exposure to brain stimulation may result in carry-over effects that could 
confound the results of this study. Participants may enroll in the study once the listed time periods have 
passed. Assessment tool(s): Participant medical history and physical (H&P).  

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smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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24.6. Arm design and procedures 
We will recruit smokers and matched non-smokers for a randomized, sham-controlled crossover study of 3 
conditions of tDCS: (1) anodal left-dlPFC + cathodal right-vmPFC stimulation, with anode over the left 
dlPFC and cathode over the right-vmPFC; (2) cathodal left-dlPFC + anodal right-vmPFC stimulation, in 
which polarity is reversed between the two electrodes, and (3) sham stimulation. These are summarized in 
Table 1. Because tDCS is MRI scanner-compatible (Wörsching et al. 2016; Johnstone, Hinson, & Stagg 2016; 
Downar & Davis 2015; Antal et al. 2014), all tDCS sessions will be performed “online” while the participant is 
in the MRI scanner. The tDCS model we will use is the neuroConn DC-Stimulator MR (neuroCare Group 
GmbH, Munchen, Germany). 
 
Smoking subjects will attend a total of 3 visits (Orientation, MRI/tDCS +Nicotine patch, and MRI/tDCS + 
Placebo patch), and non-smoking subjects will attend a total of 2 visits (Orientation and MRI/tDCS without 
patch).  Each visit day will consist of the 3 independent MRI/tDCS sessions (Figure 1), and the nature of the 
tDCS condition will be double blinded and randomized. The 3 MRI/tDCS sessions will correspond with the 3 
tDCS conditions: (1) anodal left-dlPFC + cathodal right-vmPFC; (2) cathodal left-dlPFC + anodal right-
vmPFC; and  (3) sham.  
 
Each tDCS stimulation will last approximately 25 minutes, and will occur concurrently while the participant is 
inside the MRI scanner. MRI/tDCS sessions will last about 60-100 minutes, with scans taken before, during, 
and after the tDCS stimulation (Figure 2). Each of the 3 MRI/tDCS sessions has a “core block” of scans that is 
the same across sessions. These include the Resting scan during tDCS stimulation, and task scans during and 
after stimulation. The “core block” takes about 1 hour to complete.   
 
In addition to the “core block”, the first MRI/tDCS session of the day contains a Baseline Resting Scan, and at 
least one of the MRI/tDCS sessions will contain a DTI scan (unless tDCS is found to induce artifact in DTI, see 
note on next page). The resting and DTI scans each add ~10-15 minutes to the total session time. The addition 
of these scans will not affect participant safety or study design. Only 1 DTI scan is needed per person, and 
would only be repeated if the first scan was of poor quality. 
 
The MRI/tDCS visit day will last approximately 8 hours, with the three MRI/tDCS sessions separated by an 
interscan break of about 40-60 minutes, accounting for an approximate 1.5 hour break between tDCS sessions 
(“Inter-tDCS interval”, Figure 3).  
 
Before beginning the MRI/tDCS visit days, all subjects will attend a half day orientation visit. This time will 
consist of an informed consent session, nurse assessment, task training, mock scanner session, and tDCS 
toleration test (described below). Questionnaires will also be administered during this time. If subjects cannot 
complete questionnaires on this day, they can finish them at a later visit. The visit will last about 4.5-5.5 hours. 
Some participants may take longer to answer questionnaires, complete training tasks, or other procedures, and 
in this case their visit would be extended. If structural scans are added to orientation day (see note on next 
page), the day will be extended by about 30-45 minutes. Additional time or scans on orientation day will be 
included in compensation according to the remuneration table. 
 
tDCS toleration test: Participants will experience tDCS during the orientation session using the ramp up that 
they will experience during tDCS sessions to make sure they tolerate the sensation. This will last long enough 
for them to get the full strength (2mA intensity) and to decide if they want to proceed with the experiment, i.e. 
generally less than 2 minutes but not more than 5 minutes.  During the toleration test, the participant will be 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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seated comfortably in a chair outside the scanner (mock or real) environment. This brief tDCS application 
allows the participant to experience tDCS in a comfortable environment prior to receiving tDCS in the scanner, 
ask further questions, and decide whether they wish to participate further in the study. 
 
The MRI/tDCS visit days will consist of a brief nurse visit, refresher training on fMRI tasks, and the 3 
MRI/tDCS scanning sessions. Questionnaires will also be administered on this day, prior to scanning, during the 
inter-scan breaks, and/or after the scans. The day will last about 8 hours. 
 
Smoker participants will be given nicotine patches during the study. One of these patches will be a Nicotine 
Tolerance Test Patch (see Appendix 12), and will be applied by the nurse at the orientation session. The other 
two will be blinded Study Patches containing either nicotine or placebo. The Study Patches should be applied 2 
hours prior to the first MRI/tDCS session, due to the pharmacokinetic profile of nicotine. Smoking participants 
will be given blinded study patches (Study Patch 1, Study Patch 2) to apply the morning of the MRI-tDCS 
sessions, along with an instruction sheet for how to use the patches (see Appendix 12).  
 
Smokers will attend 1 Orientation visit (half-day) and 2 MRI/tDCS visits (full days), for a total of 3 visit days 
(Figure 1A). Smokers will need to abstain for 12 hours prior to the MRI/tDCS visit days.  All 3 tDCS 
conditions will be applied during each MRI/tDCS visit day. One MRI/tDCS visit day will be completed with a 
patch containing nicotine, and the second MRI/tDCS visit day will be completed with a patch containing 
placebo. On the morning of their first imaging day, smokers will apply Study Patch 1. The content of the patch 
(nicotine or placebo) will be randomized and double-blinded. The subject will apply Study Patch 2 on the 
morning of their second MRI/tDCS visit day. 
 
Non-smokers will attend 1 Orientation visit (half-day) and 1 MRI/tDCS visit day (full day) (Figure 1B), 
without nicotine or placebo patch conditions. All 3 tDCS conditions will be applied during the MRI/tDCS visit 
day.  Non-smokers will be matched by age, gender, race, and education, as recruitment allows.  
 
To assess the online and after-effects of tDCS on the ECN, DMN and SN, the MRI/tDCS session will measure 
resting state functional connectivity, and BOLD signal and behavioral performance during cognitive fMRI 
tasks. Three classical behavioral tasks will be presented during the scanning session: (1)  Parametric Flanker 
Task known to elicit activity from the ECN and SN, (2) N-back task known to target the ECN, and (3) 
Amygdala Reactivity Task, known to be modulated by the SN and DMN. (See Section 24.8 for task details). An 
example of an MRI/tDCS session timeline is shown in Figure 2, the exact order of tasks may differ across 
participants.  
 
High-resolution structural images will be acquired on each scan day. These structural scans may include T1 
weighted, T2 weighted, or Diffusion Tensor Imaging (DTI) scans. Either a T2 weighted or a DTI scan will be 
needed for post-hoc analysis on the effect of inter-individual anatomical variability on the effects of tDCS. Pilot 
studies and discussions with modeling experts on the study team will be used to determine whether a T2 or DTI 
scan will be best suited for this analysis.  
 
Note about structural scans: Preliminary studies using a phantom in the scanner have found that some structural 
scans may be affected by the presence of tDCS electrodes. The significance of this artifact in human subjects will 
not be known until the study begins. Structural scans are important for conducting post-hoc modeling studies of 
predicted current flow through body and brain tissues such as the skull. Artifact may reduce the accuracy of these 
models. It may be necessary to collect structural scans during a separate session when the participant is not 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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wearing the tDCS device. If this is the case, the structural scans will be collected either during orientation day, or 
through Protocol 457. Additionally, if we find that the DTI scan collected with tDCS equipment is not useable, it 
will be removed from the imaging day procedures for future participants. 
 
Regulatory status for drugs/devices in the tDCS arm: 
Nicotine Patch: An IND has not been obtained for the use of the nicotine patch in this study. This study meets 
the criteria for exemption for an IND as this investigation is not intended to support a new indication for use or 
any other significant change to the labeling; the nicotine patch is already approved and marketed and the 
investigation is not intended to support a significant change in advertising; and the investigation does not 
involve a route of administration or dosage level in use in a patient population or other factor that significantly 
increases the risks associated with the use of the drug product. Individually tailored nicotine patch doses have 
been used in smoking cessation trials (Hurt, 2003) and higher doses have been shown to produce a greater 
reduction of withdrawal symptoms in heavier users (Ebbert et al., 2007) .  As stated above, nicotine patch doses 
up to 63mg/d have been shown to be safe and generally well-tolerated by smokers (Ebbert et al., 2007; Zevin, 
Jacob, & Benowitz, 1998).  
 
MRI: The 3T MRI machine used in this protocol is FDA approved and is used as a data collection tool. This study 
may use the MRI in research mode, including custom pulse sequences. All sequences, including those in research 
mode, are within FDA approved specific absorption radiation (SAR) limits. No additional risk is incurred in 
research mode, and we are not testing sequences to evaluate safety or risk.  
 
tDCS: The tDCS device weuse is the neuroConn DC-Stimulator MR (neuroCare Group GmbH, Munchen, 
Germany). This tDCS device has not been cleared by the FDA for the treatment of any medical indication, and 
it’s use in this study is investigational. tDCS does NOT meet the criteria for a significant risk device, per FDA 
21 CFR 812. The tDCS stimulation parameters used in this study follow standard safety guidelines and 
minimize risk of adverse events (Fregni et al 2015, Bikson et al 2016). The tDCS parameter boundaries are 
listed below in section 2. tDCS Parameter boundaries.  
The safety and efficacy of tDCS is well established and is not being studied in this protocol. The procedure is 
considered minimal risk based on systematic reviews of the published literature (Brunoni et al 2011; Fregni et 
al., 2015). No serious adverse events have been reported in the modern tDCS literature (since 1998). A recent 
review by an expert panel has summarized and evaluated risks and the current regulatory status for tDCS based 
on data from over 400 studies (Fregni et al, 2015). As the authors state, “To our knowledge, in the US, IRBs 
ubiquitously (at a minimum overwhelmingly) designate tDCS trials NSR […], thus not requiring a formal IDE 
application to the FDA.” (Fregni et al, 2015, p10). In cases where investigators have asked the FDA for a risk-
designation for a tDCS study, the FDA has considered tDCS trials as NSR (Fregni et al, 2015). 
 
Repeat visits & alternative scheduling: Sometimes, due to equipment or scheduling issues, or poor data quality, 
subjects may need schedule an additional visit(s) to finish or repeat study procedures. Smokers who repeat scan 
days will also repeat the Patch procedures, and will be provided the appropriate patch for the repeat visit by the 
pharmacy. Additionally, some subjects may not be able to attend an 8-hour visit day. If this is not possible, we 
will allow participants to break up MRI/tDCS sessions across days in a manner conducive to both the 
participant’s and the NRB’s scheduling needs. 
 
tDCS Assessment at the end of Imaging Day (Nonsmokers): RA, LAI, AI or study team member will remove 
the tDCS electrodes at the end of the last tDCS session. The team member will verbally assess the participant 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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for adverse reaction to tDCS, and specifically inquire about headache or skin discomfort. The team member will 
assess the site of skin application for erythema or other adverse reaction. The team member will determine 
whether a nurse, PA, or MAI needs to be alerted for medical care or further assessment. 
 
 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Figure 1: tDCS Stimulation Condition Timeline and Randomization 
 
 
 
Figure 1: tDCS stimulation condition timeline and randomization method. The three tDCS conditions are (1) anodal left 
dlPFC and cathodal right vmPFC (supra-orbital area), (2) cathodal left-dlPFC and anodal right-vmPFC (3) sham tDCS. The 
orientation visit consists of a informed consent session, nurse visit, task training, mock scanner session, and questionnaires. 
A) Visit Day timelines for smoker group. B)  Visit Day timelines for non-smoker group.  dlPFC = dorsolateral prefrontal 
cortex, vmPFC = ventromedial prefrontal cortex, A = anodal, C = cathodal. 
 
 
 
Table 1: Within-Subject Study Conditions 
Abstinent 
Smokers 
Sham 
Anodal dlPFC +  
Cathodal vmPFC 
Cathodal dlPFC +  
Anodal vmPFC 
Placebo Patch 
1. Sham / 
Placebo Patch 
2. A-dlPFC + C-vmPFC /  
Placebo Patch 
3. C-dlPFC + A-vmPFC /  
Placebo Patch 
Nicotine Patch 
4. Sham / 
Nicotine Patch 
5. A-dlPFC + C-vmPFC /  
Nicotine Patch 
6. C-dlPFC + A-vmPFC /  
Nicotine Patch 
Non-smokers 
 
 
 
No patch 
1. Sham 
2. A-dlPFC + C-vmPFC 
3. C-dlPFC + A-vmPFC 
* dlPFC = dorsolateral prefrontal cortex, vmPFC = ventromedial prefrontal cortex, A = anodal, C = cathodal. 
 
  
 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Figure 2: Sample of single MRI/tDCS session. Estimated* timeline.  
*All times are approximate and show idealized conditions. On occasion, due to equipment, compliance, or 
unforeseen disruptions, timing may change. Some scans may be repeated due to excessive motion and other 
technical problems, if it does not interfere with study design. Exact order of scanner tasks may differ from the 
order shown below. Time in magnet will never exceed 2.5 hours for one MRI/tDCS session. 
 
 
 
 
 
 
Figure 3: Inter-scan interval and Inter-tDCS interval.Estimated* timeline.  
*All times are approximate and show idealized conditions. On occasion, due to equipment, compliance, or 
unforeseen disruptions, timing may change. Some scans may be repeated due to excessive motion and other 
technical problems, if it does not interfere with study design. Time in magnet will never exceed 2.5 hours for one 
MRI/tDCS session. 
 
 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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1. Nurse Assessments for tDCS Arm: 
The nursing assessments for the tDCS Arm have been adjusted for the specific needs of the tDCS 
experiment. The nurse assessments are broken down into: Behavioral Pilot Assessments, Imaging Phase 
Assessments (Smokers), and Imaging Phase Assessments (Nonsmokers). Each of these is further broken down 
into tDCS/scanner and non-tDCS/scanner study visit assessments.  
 
For all cases, the nurse assessment will take place after the informed consent interview, prior to beginning 
the orientation or tDCS sessions. If the participant returns for another visit to complete remaining tDCS sessions 
or  orientation procedures, the nurse assessment will take place prior to beginning the other study procedures 
that are scheduled for that day. Information obtained during the nurse assessment is stored confidentially and 
securely in the individual’s medical file (See section 5.0 – Storage of Data and Samples).  
 
Behavioral Pilot Nurse Assessments: The assessments will include: 
 
Non-tDCS/Mock Scanner Visit (Orientation) 
- Breathalyzer for expired ethanol 
- Urine toxicology screen for commonly abused drugs* 
- Urine pregnancy (females) 
 
tDCS/Mock Scanner Visit 
Pre-assessment 
- Breathalyzer for expired ethanol 
- Urine toxicology screen for commonly abused drugs* 
- Urine pregnancy (females) 
- Weight & Vital Signs 
- Assess for recent caffeine intake and approximate last use of all substances, by participant self-report 
- May assist with set up of some physiological monitoring equipment (e.g. ECG electrodes, Pulse-Ox 
monitor, Respiratory belt) 
 
Imaging Phase Nurse Assessments 
 
Non-smokers: The assessment will include: 
 
Non-tDCS/MRI Visit (Orientation).  
- Breathalyzer for expired ethanol 
- Expired CO reading 
- Urine toxicology screen for commonly abused drugs* 
- Urine pregnancy (females) 
 
tDCS/MRI Visit.  
Pre-assessment 
- Breathalyzer for expired ethanol 
- Expired CO reading 
- Urine toxicology screen for commonly abused drugs* 
- Urine pregnancy (females) 
- Weight & Vital Signs 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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- Assess for recent caffeine intake and approximate last use of all substances, by participant self-report 
- Menstrual History Questionnaire (females) 
- metal/MRI Safety Screen 
-  
 
Smokers. The assessment will include: 
 
Non-tDCS/MRI Visit (Orientation).  
Pre-assessment 
- Breathalyzer for expired ethanol 
- Expired CO reading 
- Urine toxicology screen for commonly abused drugs* 
- Urine pregnancy (females) 
- Weight & Vital Signs 
- Assess for recent caffeine intake and approximate last use of all substances, by participant self-report 
- ECG, due to use of the nicotine patch 
- Placement of a nicotine test patch (Nicotine Tolerance Test, see above Section 4.4(i)4.) 
Post-assessment (only on days when the Nicotine Tolerance Patch is worn) 
- 
Removal of nicotine test patch and assessment of skin at site of patch application  
- 
If participant experiences any adverse reactions from the patch, the nurse will notify the MAI (or 
covering clinician) and obtain vital signs.  
 
tDCS/MRI Visit.  
Pre-assessment 
- Checking that participant applied the patch properly 
- Breathalyzer for expired ethanol 
- Expired CO reading 
- Urine toxicology screen for commonly abused drugs* 
- Urine pregnancy (females) 
- Weight & Vital Signs 
- Assess for recent caffeine intake and approximate last use of all substances, by participant self-report 
- Menstrual History Questionnaire (females) 
- metal/MRI Safety Screen 
Post-assessment 
- Removal of nicotine patch and assessment of skin at site of patch application 
 
*In the event of a positive drug test for marijuana, an interview to assess last drug use and a Neuromotor Drug 
Influence Evaluation (Dr. Steve Heishman, personal communication) to test for acute intoxication will be 
performed. The purpose of the nurse assessment is to provide additional screening for characteristics (e.g., 
pregnancy, positive drug tests) which would exclude an individual from participation in the study on that day. 
If participants test positive for drugs other than marijuana they will be rescheduled, however, if they test positive 
on a second occasion they will be excluded from the study. This will be made clear to the participant in the 
consent process. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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2. tDCS Parameter boundaries:  
The tDCS stimulation parameters used in this current study follow standard safety guidelines and minimize risk 
of adverse events (Fregni et al 2015, Bikson et al 2016). Parameters are summarized in Figure 3 and Table 2.  
 
• 
Number of tDCS sessions: There is no upper limit set in the literature for maximum number of tDCS 
sessions. Smoking subjects are asked to complete 6 total tDCS sessions (4 active, 2 sham), and non-
smoking subjects are asked to complete 3 total tDCS sessions (2 active, 1 sham). As there is no 
increased risk associated with participating in more total tDCS sessions (Fregni et al 2015, Bikson et 
al 2016, Russo 2013), subjects may be asked to attend an MRI/tDCS re-visit if imaging data from an 
original visit day does not pass quality control standards set by the lab. For example, one study 
reported subjects receiving >100 tDCS sessions of 30 min duration at 2 mA without adverse effects 
arising from cumulative exposure (Russo et al 2013). 
 
• 
Number of tDCS sessions per day: Subjects will be asked to complete 3 tDCS sessions per day, one 
of which is a sham. Current recommendations for tDCS are to keep active sessions to no more than 
2 per day (Fregni et al 2015). With this in mind, participants may be asked to reschedule an additional 
active or sham MRI/tDCS session if the original scan does not pass quality control standards set by 
the lab.  
 
• 
Consecutive days of tDCS sessions and separation of visit days: There is no restriction on 
consecutive days of tDCS session or minimum separation of visit days, as there are no known adverse 
effects from consecutive tDCS days. MRI/tDCS visit days will be scheduled according to participant 
and NIDA resource availability. 
 
• 
Duration of tDCS stimulation: The duration of tDCS stimulation will be approximately 25 minutes. 
This tDCS stimulation duration falls under conventional safety parameters, which extend up to 60 
minutes (Fregni et al 2015, Bikson et al 2016). Stimulation will not exceed 60 minutes at any one 
session. 
 
• 
Interval between tDCS sessions (“inter-tDCS interval): There will be an inter-tDCS interval of 
approximately 1 hour and 30 minutes between each tDCS session. Duration of inter-tDCS interval 
ranging from 1h to 2 weeks was not found to be a confounding factor in recent meta-analysis of single 
session, sham controlled anodal dlPFC tDCS (Dedoncker et al 2016).  
 
• 
Stimulation intensity: Stimulation intensity will be 2mA for the duration of the tDCS session. This 
falls within established safety parameters and standard guidelines, which extend between 1- 2mA 
(Fregni et al 2015, Bikson et al 2016). Stimulation will not exceed 2mA. 
 
• 
Stimulation polarity: Anodal tDCS is considered excitatory, while cathodal tDCS is considered 
inhibitory. Anodal stimulation will be applied to the left-dlPFC, a cortical node of the ECN; cathodal 
stimulation will be applied to the right-vmPFC (located at the supra-orbital area), a cortical node of 
the DMN. Polarity will also be reversed between the left-dlPFC and right-vmPFC.  
 
• 
tDCS electrodes: We will use standard MR-compatible tDCS electrodes. The electrodes will be 
covered with a layer of conductive paste/gel, and then applied to the participant’s skin. Non-adhesive 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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bandages or netting may be used to hold the electrodes in place. Prior to applying the electrodes, a 
standard skin preparation may be used, such as cleaning with an alcohol swab, and/or using a mildly 
abrasive gel. 
 
• 
Placement of tDCS electrodes: Placement of the dlPFC electrode will be accomplished by the 10-
20 EEG system and/or the Beam method (Beam et al 2009), involving a set of scalp measurements 
to localize the F3 site. The vmPFC location will be estimated by the supraorbital edge. 
 
• 
Sham tDCS: One of the benefits of tDCS for experimental research is its effective sham condition. 
Participants become desensitized to the mild tingling sensation of tDCS after the initial ramping of 
current (Woods et al 2016). To simulate the experience of tDCS stimulation, current is ramped on 
and turned off at the beginning and end of the tDCS session. An additional sham option is to have 
the current ramp up and down only at the beginning of the sham session, and not at the end. This 
second sham is supported in the literature as an effective blinding technique, which subjects cannot 
distinguish from active stimulation (Gandiga et al 2006, Brunoni et al 2012). One of these sham 
options will be used for data that will be analyzed together, to be determined based on equipment 
capabilities and preliminary analysis of blinding efficacy in our cross over design. We will assess the 
efficacy of sham condition by providing participants and the investigator with a questionnaire on the 
MRI/tDCS session, wherein they will report whether they thought the tDCS session was active or 
sham (see Appendix 13). The MRI operator (or other non protocol personnel) will control active/sham 
conditions. 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Table 2: tDCS Parameter Summary 
 
Parameters 
Number of MRI/tDCS Visit Days 
Smokers: 2 
Non-smokers: 1 
 
Number of tDCS sessions 
Smokers: 6 (4 active, 2 sham) 
Nonsmokers: 3 (2 active, 1 sham) 
 
Number of tDCS sessions/day 
3 tDCS sessions/day 
Duration of tDCS 
~ 25 minutes/tDCS session, not to exceed 60 min 
Inter-tDCS interval 
~ 90 min interval between tDCS sessions 
Stimulation intensity 
2 mA 
Stimulation polarity 
Anodal dlPFC + Cathodal vmPFC (supra-orbital area) 
Cathodal dlPFC + Anodal vmPFC (supra-orbital area) 
 
Placement of electrodes 
dlPFC: 10-20 EEG system, Beam method 
vmPFC: Supra orbital edge 
 
Sham procedure 
On/Off ramp at tDCS session start  (+/- end, see 
above) 
 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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3. Characterization Measures 
The following questionnaires will be administered to participants either during the screening process, orientation 
visit, or during the imaging visit days as time permits. State measures will be collected at each visit.  Trait 
measures will be collected during orientation, but may also be completed as time allows during subsequent study 
visits. Content for each questionnaire can be found in Appendix 3 (Characterization Measures) or Appendix 4 
(State Measures). 
 
Some of these questionnaires are also found in NIDA-IRP protocol 10-DA-N457. If the participant has enrolled 
in 10-DA-N457, these questionnaires may be completed within that protocol instead. These include: Fagerstrom 
Test for Nicotine Dependence, Toronto Alexithymia Scale (TAS-20), Adult ADHD Self-Report Scale, Positive 
and Negative Affect Schedule, and the State-Trait Anxiety Inventory Form Y (STAI). Questionnaires answered 
during screening, under Protocol 415, may also be included for analysis. Questionnaires do not need to be repeated 
on visit days if they were conducted during either the screening Protocol 415 or Protocol 457. 
 
Nicotine Dependence and Withdrawal Scales (Smoking Group Only). 
1) The Fagerström Test for Nicotine Dependence (Heatherton et al., 1991) is a six item test that measures the 
severity of nicotine addiction on a 0-10 scale. To be completed once (Trait). Completion time: ~2-3min. 
2) The Tobacco Craving Questionnaire (Singleton et al., 2003) is a brief instrument used to assess current 
feelings related to smoking and craving using 12 Likert-type items.  Each item is rated on a 7-point scale from 
strongly disagree to strongly agree. To be completed each visit (State).  Completion time: ~2min.  
3) Tobacco Craving Scale consists of 5 self-report items pertaining to desire for a cigarette that are rated on a 
10-point scales. To be completed each visit (State). Completion time:  ~2 min. 
4) The Minnesota Nicotine Withdrawal scale (Hughes and Hatsukami, 1998) yields a measure of total 
withdrawal related discomfort based on ratings from 15 self-report items. To be completed each visit (State).  
Completion time: ~2-3 min. 
5) The Cigarette Dependence Scale (Etter et al., 2003) is a brief 12-item self report that assesses the main 
components of DSM-IV and ICD-10 definitions of dependence, which include compulsion, withdrawal 
symptoms, loss of control, time allocation, neglect of other activities, and persistence despite harm. To be 
completed once (Trait). Completion time: ~2-3min.    
6) A general smoking history questionnaire will also be administered. To be completed once (Trait). 
Completion time: ~5 min. 
7) Visual analog scales (100mm) will also be used to assess the degree to which various nicotine withdrawal 
symptoms may be experienced: depressed, clumsy, tired, anxious, happy, drowsy, sad, dizzy, alert, energetic, 
light-headed, irritable, frustrated, nervous, sad, desire to smoke, difficulty concentrating, increased appetite, 
hunger, weight-gain, difficultly sleeping, awakening at night, insomnia, restless, impatient, panicked, missing 
cigarettes, urge to smoke, disoriented. To be completed each visit (State).  Completion time ~10 min. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Personality Scales 
8) The Brief Externalizing Inventory (Hall et al., 2007), adapted from the full Externalizing inventory 
(Krueger et al., 2007), is a subset of 159 self-report items used to assess a range of behavioral and personality 
characteristics that have been attributed to a broad psychological construct termed externalizing.  These 
characteristics include: physical/relational destructive-aggression, boredom proneness, irresponsibility, 
problematic impulsivity, drug and alcohol use or problems, theft, fraud, rebelliousness, alienation, and blame 
externalizing.  A recent report demonstrated that participants rated as high externalizing displayed reduced 
amplitude ERNs in comparison to those with low externalizing scores (Hall et al., 2007).  Thus, this measure 
may be a useful tool to account for variability in ERN amplitudes. To be completed once (Trait). Completion 
time: ~15min.     
9) The Temperament and Character Inventory (Cloninger et al., 1994) is a widely used test that assesses 
dimensions of personality (e.g., harm avoidance, novelty seeking, reward dependence, and persistence) that 
are considered to be related to monoaminergic function. To be completed once (Trait).  Completion time: 
~25min. 
10) Sensation Seeking Scale V (SSS-V, Zuckerman et al., 1978) is a 40-item self-report questionnaire that 
assesses individual differences in sensation seeking.  The scale consists of four subscales (Boredom 
Susceptibility [BS], Thrill and adventure seeking [TAS], Experience seeking [ES], and Disinhibition [Dis]) 
composed of 10-items each. To be completed once (Trait). Completion time: ~5-10min. 
11) Attitudes Towards Risk Questionnaire consists of 34-self report items rated on a 5-point Likert scale that 
assess attitudes towards physical and psychological risk. To be completed once (Trait). Completion time: ~5-
10min. 
12) Toronto Alexithymia Scale (TAS-20): a 20-item self report questionnaire to assess emotional awareness To 
be completed once (Trait). Completion time: ~5min. 
13) Adult ADHD Self-Report Scale (ASRS v1.1): This questionnaire is an 18-item checklist designed by the 
World Health Organization (WHO) and Workgroup on Adult ADHD to determine whether a participant is 
suffering from symptoms related to attention-deficit/hyperactivity disorder. To be completed once (Trait). 
Completion time: ~ 5 minutes. 
Mood and Anxiety Scales 
14) The Positive and Negative Affect Schedule (or similar self-rated questionnaire) is a 20-item scale composed 
of 10 items describing negative affect and 10 items describing positive affect. To be completed each visit 
(State).  Completion time: ~5 min. 
15) Profile of Mood States (POMS) measures present mood state by a list of adjectives on a 5-point Likert scale 
and measures six dimensions of affect, including tension-anxiety, depression-dejection, anger-hostility, 
vigor-activity, fatigue-inertia, and confusion-bewilderment. The measure has been shown to produce reliable 
and valid profiles of mood state (McNair et al., ; Cella et al., 1989).  To be completed each visit (State). 
Completion time ~5min. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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16) The State-Trait Anxiety Inventory Form Y (STAI) is an instrument for measuring anxiety in adults. The 
STAI differentiates between the temporary condition of "state anxiety" and the more general and long-
standing quality of "trait anxiety." The essential qualities evaluated by the STAI-anxiety scale are feelings of 
apprehension, tension, nervousness, and worry. Form Y1 (State): To be completed each visit. Form Y2 
(Trait): To be completed once. Completion time: ~5min each. 
17) Beck Depression Inventory-II (BDI-II: Beck et al., 1996): a brief self-administered inventory that assesses 
depressive symptoms both at the time of completion and in the preceding 7 days.  The NIDA-IRP Critical 
Alert System will be activated in CDW to address critical responses to this questionnaire. To be completed 
once (Trait).  Completion time: ~5 min.  
18) Beck Anxiety Inventory (Beck et al., 1988): a brief inventory similar to the BDI-II that assesses symptoms 
of anxiety. To be completed once (Trait).  Completion time: ~5 min. 
 
Other Drug Use 
19) Drug Use Survey (DUS): An interviewer-administered questionnaire designed to collect information 
regarding lifetime history of substance use. To be completed once (Trait). Completion time: < 40 minutes. 
 
Other Questionnaires 
20) Menstrual Cycle History Questionnaire: To be administered at the start of each visit. Menstrual cycle 
information can be used to better understand gender differences should they be apparent in this study. To be 
completed each imaging visit (State). Completion time  ~2 min. 
 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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24.7 tDCS Behavioral Pilot 
 
Up to the first 25 non-smoking participants will participate in the tDCS Behavioral Pilot. This pilot 
study will examine the effect of tDCS stimulation on behavioral outcomes on the Parametric Flanker, 
N-back, and Amygdala Reactivity tasks in the mock scanner. All procedures will be done as 
currently approved in the main tDCS study, except without MRI (i.e. in the mock scanner only) or 
characterization questionnaires (only the tDCS Blinding Questionnaire will be administered to this 
group).  
 
The tDCS Pilot will consist of an Orientation Session (consent, nurse visit, and task training, 
questionnaires and tDCS toleration test; approximately 3.5 hours), and three Mock Scanner/tDCS 
sessions (same as below, 24.6:Arm Design and Procedures; approximately 5 - 6 hours for three 1-hour 
mock scanner sessions separated by two 1-hour breaks). The orientation and each of the 3 mock 
scanner/tDCS sessions can be completed on the same or separate days, as NRB resources and 
participant scheduling allow.  
 
Because the Pilot will not include real MRI, the mock/scanner tDCS session will not include time for 
resting scans or structural image scans. The order of tasks will be re-arranged to reflect this difference. 
Order of tasks does not affect participant safety or study design. tDCS stimulation parameters will be 
the same as currently approved in the main tDCS protocol. 
 
During orientation, participants will complete the following questionnaires: (1) Brief Externalizing 
Inventory, (2) Temperament and Character Inventory, (3) Sensation Seeking Scale V, (4) State-Trait 
Anxiety Inventory Form Y (STAI), (5) Attitudes Towards Risk Questionnaire. These questionnaires 
collectively take ~60 min to complete. We may also analyze questionnaires completed by the 
participant, when the data was collected from Protocols 457 and/or 415. These data will be used to 
explore how individual differences affect response to tDCS. 
 
Participants in the pilot will be compensated at the same rate as described in the main tDCS protocol, 
minus the MRI compensation. They will still receive the completion bonus. Pilot participants can make 
up to about $258.50 if all sessions are completed on the same day. If sessions are completed on separate 
days, compensation will vary according to the remuneration table (see below, 24.18: Research and 
Travel Compensation). 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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24.8. fMRI tasks 
 
1. Parametric Flanker Task to Engage the Executive Control Network and the Salience Network: 
Participants will complete a modified Flanker task designed to yield sufficient commission errors to 
study EEG and fMRI signatures of error processing (e.g., de Bruijn et al., 2004).  In this task, 
participants respond according to the central item/letter (e.g., H or S) of a congruent (HHHHHHH or 
SSSSSSS) or incongruent (HHHSHHH or SSSHSSS) stimulus array.  Parametric manipulations of 
the agreement between flankers and the central target (e.g. SSSHSSS > SSHHHSS > SHHHHHS > 
HHHHHHH) elict varying degrees of response conflict (e.g. SSSHSSS > SSHHHSS > SHHHHHS 
> HHHHHHH) as measured by speed and accuracy of response. Participants are presented with a 
fixation mark on the screen, after which the stimulus array appears.  Participants are instructed to 
respond as fast as possible to avoid feedback indicating that their response was too slow according to 
a preset reaction time (RT) deadline.  Whenever participants respond too slowly, feedback (e.g., “!”) 
is presented indicating that they need to respond faster on future trials.  This task measures areas (e.g. 
the anterior cingulate) thought to be involved in error monitoring or conflict and has been shown to 
be dysfunctional in drug abusers. 
 
2. N-back Task to Engage the Executive Control Network: The n-back task is designed to parametrically 
manipulate working memory (WM) by asking participants to determine whether a given stimulus 
matches a stimulus presented in either the previous trial (1-back), 2 trials previously (2-back), or 3 
trials previously (3-back).  To control for attentional mechanisms, participants may also complete 
either 0-back trials, in which they simply respond to a predefined stimulus item; 1-back trials can 
also be chosen as a control condition.  Variants of this task have been previously published (Cohen 
et al, 1994). The N-back task is known to engage the Executive Control Network (ECN) (Lerman et 
al 2014).  
 
In the version of the task used for this study, we will choose only 2 of the parametric conditions, e.g. 
1-back and 3-back. This will reduce the time required to complete the task so that it can fit within the 
relatively short 1-hour MRI scan session. The task will be given both during and after tDCS 
stimulation, to compare the “online” (during) and “offline” (after-effects) of stimulation on working 
memory. 
 
3. Amygdala Reactivity Task to measure top-down control: Participants will complete a simple 
perceptual task previously shown to produce robust bilateral amygdala activity (Hariri et al., 2002; 
Pezawas et al., 2005; Foland-Ross et al., 2010), as well as top-down prefrontal regulation of amygdala 
reactivity (Hariri et al., 2002; Foland-Ross et al., 2010).  This blocked fMRI paradigm consists of 
two experimental conditions: a face matching condition, and a sensorimotor control condition. During 
the emotion matching condition, participants view an array of 3 faces and their task is to select (via 
button press) one of the two faces that matches the identity of the target face. During the sensorimotor 
control task, participants view an array of 3 geometric shapes (circles, vertical and horizontal ellipses) 
and select one of the two shapes (bottom) that matches the target shape (top).  Each block begins with 
a brief instruction screen (“match face” or “match shape”). During each emotion block different 
images selected from a standard set of pictures of facial affect (Ekman and Friesen, 1976), are 
presented. During the control blocks, different geometric shapes are presented in a pseudo-random 
order.     

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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24.9. Storage of data and Data Sharing Plan 
The same as outlined for above in Section 5., Storage of data and samples and Section 6., Data Sharing Plan. 
24.10. Risks/discomforts 
tDCS: tDCS is a minimal risk procedure and side effects are mild, benign and transient. These may include 
itching, tingling, a reddening or a burning sensation of the skin underneath the stimulating electrode. These 
symptoms are deemed a discomfort in 10-14% of tDCS participants (Brunoni et al 2011). In order to further 
reduce these minimal risks, the impedance of the tDCS system will be continuously monitored throughout all 
sessions. Instead of the more popular sponge pads soaked in saline, bicarbon electrodes with a thick layer of 
conductive paste will be used to prevent drying of the electrodes during the scanning session. The stimulator will 
automatically turn off when the impedance value exceeds the critical range.   
 
If discomforts occur, a decision will be made by the MAI or designate about whether the participant should 
complete the experiment. Headaches can also be a side effect of tDCS, and these subside with removal of the 
strap or with non-prescription medication. Non-prescription pain killers will be provided as decided on a case-
by-case basis by the MAI.  
 
Nicotine patch: the risks/discomforts of the nicotine patch are the same as listed in Section 8.2. Nicotine Patch 
Administration. Only the smoker group will receive nicotine patches. Nicotine dose will be individually tailored 
based on the average number of cigarettes smoked per day to better match daily nicotine intake. The following 
graded dosing scale will be used to individually tailor nicotine dose, it is identical to the scale found in Section 
4.1, Study Overview: 
 
“Nicotine administration during abstinence and scanning sessions. Two essential pharmacological issues 
that are of importance in the current design relate to participant’s recent exposure to nicotine and to the 
selected dose administered to each participant. Doses will be individually tailored based on the average 
number of cigarettes smoked per day to better match daily nicotine intake (please see “Individually 
Tailored Nicotine Dose” in Section 1.5 above for justification). The dose for smokers consuming less 
than 10 cigarettes per day will be 14mg to start. If they are still experiencing cravings their dose will be 
increased to 21mg . The dose for smokers consuming 10-15 cigarettes per day will be a 21mg patch and 
this will be increased by 7mg for each additional 5 cigarettes/day (i.e., 21mg for individuals who smoked 
10-15 cigarettes/day; 28mg for 15-20 cigarettes; 35mg for 20-25 cigarettes, and 42mg for more than 25 
cigarettes).  Nicotine patch doses up to 63mg/d have been shown to be safe and generally well-tolerated 
by smokers (Ebbert et al., 2007; Zevin, Jacob, & Benowitz, 1998).” 
24.11. Outcome measures 
There are no additional outcome measures beyond those described in the main protocol document (Sections 10.1 
and 10.2).  

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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24.12. Statistical Analysis 
The same as outlined above in Section 11, with the addition of the following power analyses. 
 
Power Analysis. 
 
General fMRI power. The same as outlined above in Section 11.4: “Since fMRI is the main outcome 
measurement utilized in the present protocol, the key power analysis pertains to the fMRI data.  However, 
prospective power analyses for fMRI data are complicated for several reasons. First, fMRI data are 
analyzed in a hierarchal manner such that both the intra-participant variance from the time-course data 
and the inter-participant variance across individuals could affect statistical power. A large number of time 
points tend to mitigate effects of intra-participant variance, but temporal autocorrelation and scanner 
limitations limit the number of independent measurements per unit time and thus the number of 
independent time points that are collected.  In addition, effect sizes and both types of variance will vary 
spatially.  Because of this, a given study may have sufficient power to detect differences in some brain 
regions, but lack sufficient power in other regions where the null hypothesis is false. Finally, fMRI 
analysis consists of a very large number of non-independent multiple comparisons, greater than 1.5 
million at the group level, necessitating correction methods less severe than a Bonferroni correction, as 
discussed above. Thus, a proper power analysis on fMRI data requires simulating all of these effects. 
Desmond and Glover (2002) have performed such a simulation.  They show for a relatively modest signal 
change of 0.5% during a cognitive task and with an intra-participant standard deviation of 0.75% and an 
inter-participant standard deviation of 0.5%, that 11 participants are required for a power of 0.8 using p 
< 0.05.  Using a false positive rate of p < 0.002, a level more consistent with a cluster size threshold to 
correct for multiple comparisons (Forman et al., 1995), and with the variances kept the same, 
approximately 21 participants are needed for an expected signal change of 0.5% and 11 
participants for a signal change of 0.75%.  For more than ~100 independent time points, power (and 
hence the intra-participant variance) is relatively independent of the number of time points (Desmond and 
Glover, 2002).  All current analyses should fall within this range.” 
 
tDCS power. Based on laboratory experience and literature review, the effect size for tDCS is the smallest 
of the study manipulations. Therefore, sample sizes falling in the appropriate power range for tDCS will 
also fall within the appropriate range for other manipulations in this study. In a meta-analytic review of 
the effect of tDCS on behavioral performance in cognitive tasks, it was found that effect size (Cohen’s d) 
for anodal stimulation was about 0.49 (CI: 0.26, 0.72) for tDCS vs sham stimulation (Jacobson, 
Koslowsky, & Lavidor, 2011). Cathodal tDCS has been found to produce less reliable effect sizes than 
anodal tDCS. There is no independent cathodal tDCS condition in this study, and we have calculated 
power for anodal tDCS stimulation. The direction of tDCS effect is not classically known across each of 
the task paradigms, thus a two-tailed dependent samples t-test will be used. Approximately 34 
participants in each group (smoker and non-smoker) will be required to achieve a power of 0.8 
when assessing the effect of anodal tDCS vs sham (α-level of 0.05, two-tailed, matched pairs t-test, 
assuming a medium effect size for anodal tDCS stimulation).  
 
Accounting for non-completion and non-compliance, we are requesting a maximum accrual of 60 
smokers (enrollees) and 55 non-smokers (enrollees), with expectation of 35 completers in the smoker 
group, and 45 completers in the nonsmoker group. 

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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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24.13. Human subjects protection 
The same as outlined for above in Section 12. Human Subjects Protection. Enrollment for the tDCS Arm is 
shown below: 
 
APPROVED STUDY POPULATION 
 
FEMALE 
ENROLLMENT 
MALE 
ENROLLMENT 
TOTAL 
ENROLLMENT 
FEMALE 
COMPLETERS 
MALE 
COMPLETERS 
TOTAL 
COMPLETERS 
APPROVED 
CEILING 
57 
58 
115 
40 
40 
80 
NIH TARGETED/PLANNED ENROLLMENT 
 
ETHNIC CATEGORY 
Sex/Gender 
 
Females 
Males 
Total 
Hispanic or Latino 
2 
3 
5** 
Not Hispanic or Latino 
55 
55 
110 
Ethnic Category: Total of All Subjects* 
 
 
115* 
RACIAL CATEGORIES 
 
 
 
American Indian/Alaska Native 
1 
1 
2 
Asian 
1 
2 
3 
Native Hawaiian or Other Pacific Islander 
0 
0 
0 
Black or African American 
37 
37 
74 
White 
18 
18 
36 
Racial Categories: Total of All Subjects* 
57 
58 
115* 
*The “Ethnic Category: Total of All Subjects” must be equal to the “Racial Categories: total of All Subjects.” 
 
 
24.14. Anticipated benefit 
There are no direct health benefits to subjects in this arm of the study.  The results of this work may benefit the 
health of society if the findings contribute to a better understanding of brain function as related to drug abuse. 
24.15. Consent documents/process 
The consent process is the same as for the main protocol Section 15 Consent documents/Procedure, however a 
different quiz will be used that contains information relevant to the tDCS Arm. Please see attached Consent Forms 
D1 (Smokers) and D2 (Nonsmokers), and Quizzes D1 (Smokers) and D2 (Nonsmokers). As in the main protocol, 
participants must answer at least 80% of questions correctly with correct answers to #5 and #9.  
 
24.16. Data and Safety Monitoring. Quality Assurance. Adverse event and unanticipated problem 
reporting.  Alternatives to participation. Confidentiality. Conflict of Interest. 
The same as those outlined within Sections 16-21 of the main protocol document. 
24.17. Research and Travel Compensation. 
Subjects will be compensated according to NIDA-IRP guidelines below: 
 
Item 
Compensation Rate 
IRP Study Visit 
$20/hour 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
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                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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fMRI 
$15 per scan session 
tDCS 
$10 per tDCS session 
Travel to NIDA 
$15 round-trip per visit day, or 
Full reimbursement based on receipts & prior permission 
Total Completion 
10% completion bonus, paid on completion of final visit 
 
Subjects who voluntarily withdraw before completing the study will only be paid for that part of the study they 
complete.  However, if medical reasons preclude completion of the study, subjects will be paid the full stipend 
for that day.  
 
Based on MRI/tDCS scanning visits (each lasting approximately 8 hours) and a single half-day pre-assessment 
session (4 hours), anticipated approximate maximum compensation for participation in the Transcranial Direct 
Current Stimulation (tDCS) Arm of this study is about $654.50 for smokers (3 total visit days) and about $379.50 
for non-smokers (2 total visit days). 
 
Remuneration will generally be in cash payments. If participation runs into the evening or occurs on 
weekends, payment by check may be the only option. In the rare instances where a check is pre-issued because 
procedures are scheduled in the evenings or on weekends, the amount due to the participant will be estimated on 
the low end, and the participant will receive any balance due either at their next session, or via a check in the mail 
(whichever they prefer). Participants who complete and MRI session will also be offered a T-shirt with an ironed-
on picture of their brain as part of their compensation.  
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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References 
Ahs, F., Sollers, J. J., Furmark, T., Fredrikson, M., & Thayer, J. F. (2009). High-frequency heart rate variability 
and cortico-striatal activity in men and women with social phobia. NeuroImage, 47(3), 815–20. 
doi:10.1016/j.neuroimage.2009.05.091 
Al-Adawi, S., & Powell, J. H. (1997). The influence of smoking on reward responsiveness and cognitive 
functions: a natural experiment. ADDICTION, 92(12), 1773–1782. Retrieved from 
http://apps.isiknowledge.com/full_record.do?product=WOS&search_mode=GeneralSearch&qid=12&SID
=4DgiL19kHkaLFElcoPp&page=1&doc=1 
American Psychiatric Association. (1994). Diagnostic Stastistical Manual of Mental Disorders (4th ed). 
Washington DC: Author. 
Amodio, D. M., & Frith, C. D. (2006). Meeting of minds: the medial frontal cortex and social cognition. Nature 
Reviews Neuroscience, 7(4), 268–77. doi:10.1038/nrn1884 
Antal, A., Bikson, M., Datta, A., Lafon, B., Dechent, P., Parra, L. C., & Paulus, W. (2014). Imaging artifacts 
induced by electrical stimulation during conventional fMRI of the brain. NeuroImage, 85, Part 3, 1040–
1047. https://doi.org/10.1016/j.neuroimage.2012.10.026 
Aubin, H.-J., Bobak, A., Britton, J. R., Oncken, C., Billing, C. B., Gong, J., … Reeves, K. R. (2008). 
Varenicline versus transdermal nicotine patch for smoking cessation: results from a randomised open-label 
trial. Thorax, 63(8), 717–24. doi:10.1136/thx.2007.090647 
Azizian, A., Nestor, L. J., Payer, D., Monterosso, J. R., Brody, A. L., & London, E. D. (2010a). Smoking 
reduces conflict-related anterior cingulate activity in abstinent cigarette smokers performing a Stroop task. 
Neuropharmacology, 35(3), 775–82. doi:10.1038/npp.2009.186 
Azizian, A., Nestor, L. J., Payer, D., Monterosso, J. R., Brody, A. L., & London, E. D. (2010b). Smoking 
reduces conflict-related anterior cingulate activity in abstinent cigarette smokers performing a Stroop task. 
Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology, 
35(3), 775–82. doi:10.1038/npp.2009.186 
Beaver, J. D., Long, C. J., Cole, D. M., Durcan, M. J., Bannon, L. C., Mishra, R. G., & Matthews, P. M. (2011). 
The Effects of nicotine replacement on cognitive brain activity during smoking withdrawal studied with 
simultaneous fMRI/EEG. Neuropharmacology, 36(9), 1792–800. doi:10.1038/npp.2011.53 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 106 of 221 
 
 
Beam, W., Borckardt, J. J., Reeves, S. T., & George, M. S. (2009). An efficient and accurate new method for 
locating the F3 position for prefrontal TMS applications. Brain Stimulation, 2(1), 50–54. 
https://doi.org/10.1016/j.brs.2008.09.006 
Beck AT, Epstein N, Brown G, Steer RA (1988) An inventory for measuring clinical anxiety: psychometric 
properties. J Consult Clin Psychol 56:893-897. 
 
Beck AT, Steer RA, Ball R, Ranieri W (1996) Comparison of Beck Depression Inventories -IA and -II in 
psychiatric outpatients. J Pers Assess 67:588-597. 
Bickel, W. K., Odum, a L., & Madden, G. J. (1999). Impulsivity and cigarette smoking: delay discounting in 
current, never, and ex-smokers. Psychopharmacology, 146(4), 447–54. Retrieved from 
http://www.ncbi.nlm.nih.gov/pubmed/10550495 
Bikson, M., Grossman, P., Thomas, C., Zannou, A. L., Jiang, J., Adnan, T., … Woods, A. J. (2016). Safety of 
Transcranial Direct Current Stimulation: Evidence Based Update 2016. Brain Stimulation. 
https://doi.org/10.1016/j.brs.2016.06.004 
Bilkei-Gorzo, A., Rácz, I., Michel, K., Darvas, M., Maldonado, R., & Zimmer, A. (2008). A common genetic 
predisposition to stress sensitivity and stress-induced nicotine craving. Biological Psychiatry, 63(2), 164–
71. doi:10.1016/j.biopsych.2007.02.010 
Botvinick, M., Nystrom, L. E., Fissell, K., Carter, C. S., & Cohen, J. D. (1999). Conflict monitoring versus 
selection-for-action in anterior cingulate cortex. Nature, 402(6758), 179–81. doi:10.1038/46035 
Breese, C. R., Marks, M. J., Logel, J., Adams, C. E., Sullivan, B., Collins, A. C., & Leonard, S. (1997). Effect 
of Smoking History on [3H]Nicotine Binding in Human Postmortem Brain. J. Pharmacol. Exp. Ther., 
282(1), 7–13. Retrieved from http://jpet.aspetjournals.org/cgi/content/abstract/282/1/7 
Brody AL (2006) Functional brain imaging of tobacco use and dependence. J Psychiatr Res 40:404-418. 
Broyd, S. J., Demanuele, C., Debener, S., Helps, S. K., James, C. J., & Sonuga-Barke, E. J. S. (2009). Default-
mode brain dysfunction in mental disorders: a systematic review. Neuroscience and Biobehavioral 
Reviews, 33(3), 279–96. doi:10.1016/j.neubiorev.2008.09.002 
Bruijnzeel, A. W., Prado, M., & Isaac, S. (2009). Corticotropin-releasing factor-1 receptor activation mediates 
nicotine withdrawal-induced deficit in brain reward function and stress-induced relapse. Biological 
Psychiatry, 66(2), 110–7. doi:10.1016/j.biopsych.2009.01.010 
Brunoni, A. R., Amadera, J., Berbel, B., Volz, M. S., Rizzerio, B. G., & Fregni, F. (2011). A systematic review 
on reporting and assessment of adverse effects associated with transcranial direct current stimulation. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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page 107 of 221 
 
 
International Journal of Neuropsychopharmacology, 14(8), 1133–1145. 
https://doi.org/10.1017/S1461145710001690 
Brunoni, A. R., Nitsche, M. A., Bolognini, N., Bikson, M., Wagner, T., Merabet, L., … Fregni, F. (2012). 
Clinical research with transcranial direct current stimulation (tDCS): Challenges and future directions. 
Brain Stimulation, 5(3), 175–195. https://doi.org/10.1016/j.brs.2011.03.002 
Buckner, R. L., Andrews-Hanna, J. R., & Schacter, D. L. (2008). The brain’s default network: anatomy, 
function, and relevance to disease. Annals of the New York Academy of Sciences, 1124, 1–38. 
doi:10.1196/annals.1440.011 
Buisson, B., & Bertrand, D. (2002). Nicotine addiction: the possible role of functional upregulation. Trends in 
Pharmacological Sciences, 23(3), 130–136. doi:10.1016/S0165-6147(00)01979-9 
Bush, G., & Shin, L. M. (2006). The Multi-Source Interference Task: an fMRI task that reliably activates the 
cingulo-frontal-parietal cognitive/attention network. Nature Protocols, 1(1), 308–13. 
doi:10.1038/nprot.2006.48 
Bush, G., Shin, L. M., Holmes, J., Rosen, B. R., & Vogt, B. A. (2003). The Multi-Source Interference Task: 
validation study with fMRI in individual subjects. Molecular Psychiatry, 8(1), 60–70. 
doi:10.1038/sj.mp.4001217 
Cahill, K., Stevens, S., Perera, R., & Lancaster, T. (2013). Pharmacological interventions for smoking cessation: 
an overview and network meta-analysis. In Cochrane Database of Systematic Reviews. John Wiley & 
Sons, Ltd. Retrieved from http://onlinelibrary.wiley.com/doi/10.1002/14651858.CD009329.pub2/abstract 
Cahill K, Stevens S, & Lancaster T. (2014). PHarmacological treatments for smoking cessation. JAMA, 311(2), 
193–194. https://doi.org/10.1001/jama.2013.283787 
Caspi, A., Hariri, A. R., Holmes, A., Uher, R., & Moffitt, T. E. (2010). Genetic sensitivity to the environment: 
the case of the serotonin transporter gene and its implications for studying complex diseases and traits. 
American Journal Of Psychiatry, 167(5), 509–27. doi:10.1176/appi.ajp.2010.09101452 
Cavanagh, J. F., Cohen, M. X., & Allen, J. J. B. (2009). Prelude to and resolution of an error: EEG phase 
synchrony reveals cognitive control dynamics during action monitoring. Journal Of Neuroscience, 29(1), 
98–105. doi:10.1523/JNEUROSCI.4137-08.2009 
CDC Smoking and Tobacco Use; Fact Sheet; Adult Cigarette Smoking in the United States; (2016, March 14). 
Retrieved September 17, 2016, from 
http://www.cdc.gov/tobacco/data_statistics/fact_sheets/adult_data/cig_smoking/ 

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smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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page 108 of 221 
 
 
Center for Substance Abuse Treatment. (1999). Enhancing Motivation for Change in Substance Abuse 
Treatment. Rockville, MD: Substance Abuse and Mental Health Services Administration. 
Chapman, L. J., & Chapman, J. P. (1978). Revised physical anhedonia scale. Unpublished Test. 
Cloninger, C. R., Przybeck, T. R., & Svrakic, D. M. (1994). The Temperament and Character Inventory (TCI): 
A guide to its development and use. St. Louis, MO: Center for Psychobiology of Personality, Washington 
University. 
Cohen, H. L., Porjesz, B., Begleiter, H., & Wang, W. (1997, November). Neurophysiological Correlates of 
Response Production and Inhibition in Alcoholics. Alcoholism: Clinical and Experimental Research. 
doi:10.1111/j.1530-0277.1997.tb04468.x 
Cohen, J. (1988). Statistical power analysis for the behavioral sciences (2nd ed.). Hillsdale, NJ: Erlbaum. 
Cohen JD, Forman SD, Braver TS, Casey BJ, Servan-Schreiber D, Noll DC (1994). Activation of the prefrontal 
cortex in a nonspatial working memory task with functional MRI. Hum Brain Mapp 1: 293-304. 
Cohen, S., Kamarck, T., & Mermelstein, R. (1983). A global measure of perceived stress. Journal of Health and 
Social Behavior, 24(4), 385–96. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/6668417 
Cole, D. M., Beckmann, C. F., Long, C. J., Matthews, P. M., Durcan, M. J., & Beaver, J. D. (2010). Nicotine 
replacement in abstinent smokers improves cognitive withdrawal symptoms with modulation of resting 
brain network dynamics. NeuroImage, 52(2), 590–9. doi:10.1016/j.neuroimage.2010.04.251 
Curry, S., Wagner, E. H., & Grothaus, L. C. (1990). Intrinsic and extrinsic motivation for smoking cessation. 
Journal of Consulting and Clinical Psychology, 58(3), 310–6. Retrieved from 
http://www.ncbi.nlm.nih.gov/pubmed/2195084 
Dallery, J., & Raiff, B. R. (2007). Delay discounting predicts cigarette smoking in a laboratory model of 
abstinence reinforcement. Psychopharmacology, 190(4), 485–96. doi:10.1007/s00213-006-0627-5 
Dawkins, L., Powell, J. H., West, R., Powell, J., & Pickering, A. (2006). A double-blind placebo controlled 
experimental study of nicotine: I--effects on incentive motivation. Psychopharmacology, 189(3), 355–67. 
doi:10.1007/s00213-006-0588-8 
Dawkins L, Powell JH, West R, Powell J, Pickering A (2007b) A double-blind placebo-controlled experimental 
study of nicotine: II--Effects on response inhibition and executive functioning. Psychopharmacology (Berl) 
190:457-467. 
 

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smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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de Bruijn ER, Hulstijn W, Verkes RJ, Ruigt GS, Sabbe BG (2004) Drug-induced stimulation and suppression of 
action monitoring in healthy volunteers. Psychopharmacology (Berl) 177:151-160. 
Dedoncker, J., Brunoni, A. R., Baeken, C., & Vanderhasselt, M.-A. (2016). The effect of the interval-between-
sessions on prefrontal transcranial direct current stimulation (tDCS) on cognitive outcomes: a systematic 
review and meta-analysis. Journal of Neural Transmission, 1–14. https://doi.org/10.1007/s00702-016-
1558-x 
Doran, N., Spring, B., McChargue, D., Pergadia, M., & Richmond, M. (2004). Impulsivity and smoking relapse. 
Nicotine & Tobacco Research : Official Journal of the Society for Research on Nicotine and Tobacco, 
6(4), 641–7. doi:10.1080/14622200410001727939 
Downar, J., & Davis, K. D. (2015). Magnetic resonance imaging in neuromodulation. In C. Hamani, P. 
Holtzheimer, A. M. Lozano, & Helenyberg (Eds.), Neuromodulation in Psychiatry (pp. 49–79). John 
Wiley & Sons, Ltd. Retrieved from 
http://onlinelibrary.wiley.com/doi/10.1002/9781118801086.ch4/summary 
Drevets, W. C., Price, J. L., Bardgett, M. E., Reich, T., Todd, R. D., & Raichle, M. E. (2002). Glucose 
metabolism in the amygdala in depression: Relationship to diagnostic subtype and plasma cortisol levels. 
Pharmacology, Biochemistry, and Behavior, 71(3), 431–447. doi:10.1016/S0091-3057(01)00687-6 
Dunlop, K., Hanlon, C. A., & Downar, J. (2016). Noninvasive brain stimulation treatments for addiction and 
major depression. Annals of the New York Academy of Sciences, n/a-n/a. 
https://doi.org/10.1111/nyas.12985 
Ebbert, J. O., Dale, L. C., Patten, C. A., Croghan, I. T., Schroeder, D. R., Moyer, T. P., & Hurt, R. D. (2007). 
Effect of high-dose nicotine patch therapy on tobacco withdrawal symptoms among smokeless tobacco 
users. Nicotine & Tobacco Research : Official Journal of the Society for Research on Nicotine and 
Tobacco, 9(1), 43–52. doi:10.1080/14622200601078285 
Eckblad, M., Chapman, L. ., Chapman, J. ., & Mishlove, M. (1982). The revised social anhedonia scale. 
Unpublished Test. 
Edell WS (1995) The pscyhometric measurement of schizotypy using the Wisconsin scales of psychosis-
proneness. In: The Behavioral hig-risk paradigm in psychopathology (Miller GA, ed). New York: Springer-
verlag. 
Egner, T., Etkin, A., Gale, S., & Hirsch, J. (2008). Dissociable neural systems resolve conflict from emotional 
versus nonemotional distracters. Cerebral Cortex, 18(6), 1475–84. doi:10.1093/cercor/bhm179 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Eichele, T., Debener, S., Calhoun, V. D., Specht, K., Engel, A. K., Hugdahl, K., … Ullsperger, M. (2008). 
Prediction of human errors by maladaptive changes in event-related brain networks. Proceedings Of The 
National Academy Of Sciences Of The United States Of America, 105(16), 6173–8. 
doi:10.1073/pnas.0708965105 
Enriquez-Geppert, S., Konrad, C., Pantev, C., & Huster, R. J. (2010). Conflict and inhibition differentially 
affect the N200/P300 complex in a combined go/nogo and stop-signal task. NeuroImage, 51(2), 877–87. 
doi:10.1016/j.neuroimage.2010.02.043 
Etkin, A., Egner, T., & Kalisch, R. (2010). Emotional processing in anterior cingulate and medial prefrontal 
cortex. Trends in Cognitive Sciences, 15(2), 85–93. doi:10.1016/j.tics.2010.11.004 
Etkin, A., Egner, T., Peraza, D. M., Kandel, E. R., & Hirsch, J. (2006). Resolving emotional conflict: a role for 
the rostral anterior cingulate cortex in modulating activity in the amygdala. Neuron, 51(6), 871–82. 
doi:10.1016/j.neuron.2006.07.029 
Etkin, A., Klemenhagen, K. C., Dudman, J. T., Rogan, M. T., Hen, R., Kandel, E. R., & Hirsch, J. (2004). 
Individual differences in trait anxiety predict the response of the basolateral amygdala to unconsciously 
processed fearful faces. Neuron, 44(6), 1043–55. doi:10.1016/j.neuron.2004.12.006 
Etter, J.-F., Le Houezec, J., & Perneger, T. V. (2003). A self-administered questionnaire to measure dependence 
on cigarettes: the cigarette dependence scale. Neuropharmacology, 28(2), 359–70. 
doi:10.1038/sj.npp.1300030 
Everitt, B. J., & Robbins, T. W. (2005). Neural systems of reinforcement for drug addiction: from actions to 
habits to compulsion. Nature Neuroscience, 8(11), 1481–1489. https://doi.org/10.1038/nn1579 
Fedota, J. R., & Stein, E. A. (2015). Resting-state functional connectivity and nicotine addiction: prospects for 
biomarker development. Annals of the New York Academy of Sciences, 1349(1), 64–82. 
https://doi.org/10.1111/nyas.12882 
Fertonani, A., Ferrari, C., & Miniussi, C. (2015). What do you feel if I apply transcranial electric stimulation? 
Safety, sensations and secondary induced effects. Clinical Neurophysiology, 126(11), 2181–2188. 
https://doi.org/10.1016/j.clinph.2015.03.015 
Field, M., Santarcangelo, M., Sumnall, H., Goudie, A., & Cole, J. (2006a). Delay discounting and the 
behavioural economics of cigarette purchases in smokers: the effects of nicotine deprivation. 
Psychopharmacology, 186(2), 255–63. doi:10.1007/s00213-006-0385-4 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Field, M., Santarcangelo, M., Sumnall, H., Goudie, A., & Cole, J. (2006b). Delay discounting and the 
behavioural economics of cigarette purchases in smokers: the effects of nicotine deprivation. 
Psychopharmacology, 186(2), 255–63. doi:10.1007/s00213-006-0385-4 
Fox, M. D., Snyder, A. Z., Vincent, J. L., Corbetta, M., Van Essen, D. C., & Raichle, M. E. (2005). The human 
brain is intrinsically organized into dynamic, anticorrelated functional networks. Proceedings Of The 
National Academy Of Sciences Of The United States Of America, 102(27), 9673–8. 
doi:10.1073/pnas.0504136102 
Fox, M. D., Buckner, R. L., Liu, H., Chakravarty, M. M., Lozano, A. M., & Pascual-Leone, A. (2014). Resting-
state networks link invasive and noninvasive brain stimulation across diverse psychiatric and neurological 
diseases. Proceedings of the National Academy of Sciences, 111(41), E4367–E4375. 
Franken, I. H. a, van Strien, J. W., & Kuijpers, I. (2010). Evidence for a deficit in the salience attribution to 
errors in smokers. Drug and Alcohol Dependence, 106(2-3), 181–5. doi:10.1016/j.drugalcdep.2009.08.014 
Franklin, T., Wang, Z., Suh, J. J., Hazan, R., Cruz, J., Li, Y., … Childress, A.-R. (2011). Effects of Varenicline 
on Smoking Cue-Triggered Neural and Craving Responses. Archives of General Psychiatry, 68(5), 516–
526. doi:10.1001/archgenpsychiatry.2010.190 
Fransson, P. (2005). Spontaneous low-frequency BOLD signal fluctuations: an fMRI investigation of the 
resting-state default mode of brain function hypothesis. Human Brain Mapping, 26(1), 15–29. 
doi:10.1002/hbm.20113 
Fregni, F., Nitsche, M. A., Loo, C. K., Brunoni, A. R., Marangolo, P., Leite, J., … Bikson, M. (2015). 
Regulatory considerations for the clinical and research use of transcranial direct current stimulation 
(tDCS): Review and recommendations from an expert panel. Clinical Research and Regulatory Affairs, 
32(1), 22–35. https://doi.org/10.3109/10601333.2015.980944 
Friedman, D., Cycowicz, Y. M., & Gaeta, H. (2001). The novelty P3: an event-related brain potential (ERP) 
sign of the brain’s evaluation of novelty. Neuroscience and Biobehavioral Reviews, 25(4), 355–73. 
Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/11445140 
Fritz, M. S., & Mackinnon, D. P. (2007). Required sample size to detect the mediated effect. Psychological 
Science, 18(3), 233–9. doi:10.1111/j.1467-9280.2007.01882.x 
Gentili, C., Ricciardi, E., Gobbini, M. I., Santarelli, M. F., Haxby, J. V, Pietrini, P., & Guazzelli, M. (2009). 
Beyond amygdala: Default Mode Network activity differs between patients with social phobia and healthy 
controls. Brain Research Bulletin, 79(6), 409–13. doi:10.1016/j.brainresbull.2009.02.002 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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George, O., Ghozland, S., Azar, M. R., Cottone, P., Zorrilla, E. P., Parsons, L. H., … Koob, G. F. (2007). CRF-
CRF1 system activation mediates withdrawal-induced increases in nicotine self-administration in nicotine-
dependent rats. Proceedings Of The National Academy Of Sciences Of The United States Of America, 
104(43), 17198–203. doi:10.1073/pnas.0707585104 
Gandiga, P. C., Hummel, F. C., & Cohen, L. G. (2006). Transcranial DC stimulation (tDCS): A tool for double-
blind sham-controlled clinical studies in brain stimulation. Clinical Neurophysiology, 117(4), 845–850. 
https://doi.org/10.1016/j.clinph.2005.12.003 
Gianaros, P. J., Sheu, L. K., Matthews, K. a, Jennings, J. R., Manuck, S. B., & Hariri, A. R. (2008). Individual 
differences in stressor-evoked blood pressure reactivity vary with activation, volume, and functional 
connectivity of the amygdala. Journal Of Neuroscience, 28(4), 990–9. doi:10.1523/JNEUROSCI.3606-
07.2008 
Gilbert DG, Sugai C, Zuo Y, Rabinovich NE, McClernon FJ, Froeliger B (2007) Brain indices of nicotine's effects 
on attentional bias to smoking and emotional pictures and to task-relevant targets. Nicotine Tob Res 9:351-
363. 
 
Goldstein, R. Z., & Volkow, N. D. (2011). Dysfunction of the prefrontal cortex in addiction: neuroimaging 
findings and clinical implications. Nature Reviews Neuroscience, 12(11), 652–669. 
https://doi.org/10.1038/nrn3119 
Goudriaan, A. E., De Ruiter, M. B., Van Den Brink, W., Oosterlaan, J., & Veltman, D. J. (2010). Brain 
activation patterns associated with cue reactivity and craving in abstinent problem gamblers, heavy 
smokers and healthy controls: an fMRI study. Addiction Biology, 491–503. doi:10.1111/j.1369-
1600.2010.00242.x 
Greicius, M. D., Krasnow, B., Reiss, A. L., & Menon, V. (2003). Functional connectivity in the resting brain: a 
network analysis of the default mode hypothesis. Proceedings Of The National Academy Of Sciences Of 
The United States Of America, 100(1), 253–8. doi:10.1073/pnas.0135058100 
Groom, M. J., Cahill, J. D., Bates, A. T., Jackson, G. M., Calton, T. G., Liddle, P. F., & Hollis, C. (2010). 
Electrophysiological indices of abnormal error-processing in adolescents with attention deficit 
hyperactivity disorder (ADHD). Journal of Child Psychology and Psychiatry, and Allied Disciplines, 
51(1), 66–76. doi:10.1111/j.1469-7610.2009.02128.x 
Gunnell, D., Irvine, D., Wise, L., Davies, C., & Martin, R. M. (2009). Varenicline and suicidal behaviour: a 
cohort study based on data from the General Practice Research Database. BMJ (Clinical Research Ed.), 
339, b3805. Retrieved from 
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2755726&tool=pmcentrez&rendertype=abstra
ct 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Gusnard, D. a, Akbudak, E., Shulman, G. L., & Raichle, M. E. (2001). Medial prefrontal cortex and self-
referential mental activity: relation to a default mode of brain function. Proceedings Of The National 
Academy Of Sciences Of The United States Of America, 98(7), 4259–64. doi:10.1073/pnas.071043098 
Gusnard, D. A., & Raichle, M. E. (2001). REVIEWS SEARCHING FOR A BASELINE : FUNCTIONAL 
IMAGING AND THE. Neuroscience, 2(October). 
Hahn, B., Ross, T. J., Yang, Y., Kim, I., Huestis, M. a, & Stein, E. a. (2007). Nicotine enhances visuospatial 
attention by deactivating areas of the resting brain default network. Journal Of Neuroscience, 27(13), 
3477–89. doi:10.1523/JNEUROSCI.5129-06.2007 
Hall, J. R., Bernat, E. M., & Patrick, C. J. (2007). Externalizing Psychopathology and the Error-Related 
Negativity. Psychological Science, 18(4), 326–333. https://doi.org/10.1111/j.1467-9280.2007.01899.x 
Hampson, M., Driesen, N., Roth, J. K., Gore, J. C., & Constable, R. T. (2010). Functional connectivity between 
task-positive and task-negative brain areas and its relation to working memory performance. Magnetic 
Resonance Imaging, 28(8), 1051–1057. doi:10.1016/j.mri.2010.03.021 
Heatherton, T. F., Kozlowski, L. T., Frecker, R. C., & Fagerström, K. O. (1991). The Fagerström Test for 
Nicotine Dependence: a revision of the Fagerström Tolerance Questionnaire. British Journal of Addiction, 
86(9), 1119–1127. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/1932883 
Hester, R., & Garavan, H. (2004). Executive dysfunction in cocaine addiction: evidence for discordant frontal, 
cingulate, and cerebellar activity. Journal Of Neuroscience, 24(49), 11017–22. 
doi:10.1523/JNEUROSCI.3321-04.2004 
Hildebrand, B. E., Nomikos, G. G., Hertel, P., Schilström, B., & Svensson, T. H. (1998). Reduced dopamine 
output in the nucleus accumbens but not in the medial prefrontal cortex in rats displaying a 
mecamylamine-precipitated nicotine withdrawal syndrome. Brain Research, 779(1-2), 214–25. Retrieved 
from http://www.ncbi.nlm.nih.gov/pubmed/9473676 
Hildebrand, B. E., Panagis, G., Svensson, T. H., & Nomikos, G. G. (1999). Behavioral and biochemical 
manifestations of mecamylamine-precipitated nicotine withdrawal in the rat: role of nicotinic receptors in 
the ventral tegmental area. Neuropharmacology, 21(4), 560–74. doi:10.1016/S0893-133X(99)00055-X 
Hogle, J. M., Kaye, J. T., & Curtin, J. J. (2010). Nicotine withdrawal increases threat-induced anxiety but not 
fear: neuroadaptation in human addiction. Biological Psychiatry, 68(8), 719–25. 
doi:10.1016/j.biopsych.2010.06.003 
Hughes, J., & Hatsukami, D. K. (1998). Errors in using tobacco withdrawal scale. Tobacco Control, 7(1), 92–
93. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 114 of 221 
 
 
Hughes, J. R. (1992). Tobacco withdrawal in self-quitters. Journal of Consulting and Clinical Psychology, 
60(5), 689–697. https://doi.org/10.1037/0022-006X.60.5.689 
Hughes, J. R., Higgins, S. T., & Bickel, W. K. (1994). Nicotine withdrawal versus other drug withdrawal 
syndromes: similarities and dissimilarities. Addiction (Abingdon, England), 89(11), 1461–70. Retrieved 
from http://www.ncbi.nlm.nih.gov/pubmed/7841857 
Hughes, J. R., Keely, J., & Naud, S. (2004). Shape of the relapse curve and long-term abstinence among 
untreated smokers. Addiction, 99(1), 29–38. doi:10.1111/j.1360-0443.2004.00540.x 
Hurt, R. D. (2003). Nicotine Patch Therapy Based on Smoking Rate Followed by Bupropion for Prevention of 
Relapse to Smoking. Journal of Clinical Oncology, 21(5), 914–920. doi:10.1200/JCO.2003.08.160 
Huster, R. J., Westerhausen, R., Pantev, C., & Konrad, C. (2010). The role of the cingulate cortex as neural 
generator of the N200 and P300 in a tactile response inhibition task. Human Brain Mapping, 31(8), 1260–
71. doi:10.1002/hbm.20933 
Jacobson, L., Koslowsky, M., & Lavidor, M. (2011). tDCS polarity effects in motor and cognitive domains: a 
meta-analytical review. Experimental Brain Research, 216(1), 1–10. https://doi.org/10.1007/s00221-011-
2891-9 
Janes, A. C., Pizzagalli, D. a, Richardt, S., deB Frederick, B., Chuzi, S., Pachas, G., … Kaufman, M. J. (2010). 
Brain reactivity to smoking cues prior to smoking cessation predicts ability to maintain tobacco 
abstinence. Biological Psychiatry, 67(8), 722–9. doi:10.1016/j.biopsych.2009.12.034 
Johnstone, A., Hinson, E., & Stagg, C. J. (2016). tDCS and Magnetic Resonance Imaging. In A. Brunoni, M. 
Nitsche, & C. Loo (Eds.), Transcranial Direct Current Stimulation in Neuropsychiatric Disorders (pp. 
169–195). Springer International Publishing. Retrieved from http://link.springer.com/chapter/10.1007/978-
3-319-33967-2_10 
Kahler, C. W., Spillane, N. S., Metrik, J., Leventhal, A. M., & Monti, P. M. (2009). Sensation seeking as a 
predictor of treatment compliance and smoking cessation treatment outcomes in heavy social drinkers. 
Pharmacology, Biochemistry, and Behavior, 93(3), 285–290. doi:10.1016/j.pbb.2009.01.003 
Kaufman JN, Ross TJ, Stein EA, Garavan H (2003) Cingulate hypoactivity in cocaine users during a GO-NOGO 
task as revealed by event-related functional magnetic resonance imaging. J Neurosci 23:7839-7843. 
Keating, G. M., & Siddiqui, M. A. A. (2006). Varenicline: A Review of its Use as an Aid to Smoking Cessation 
Therapy, 20(11), 945–960. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Kellner, M., Muhtz, C., Demiralay, C., Husemann, J., Koelsch, W., Yassouridis, A., & Wiedemann, K. (2009). 
The selective serotonin re-uptake inhibitor escitalopram modulates the panic response to cholecystokinin 
tetrapeptide in healthy men depending on 5-HTTLPR genotype. Journal of Psychiatric Research, 43(6), 
642–8. doi:10.1016/j.jpsychires.2008.09.001 
Kenny, P. J., & Markou, A. (2001). Neurobiology of the nicotine withdrawal syndrome. Pharmacology, 
Biochemistry, and Behavior, 70(4), 531–49. Retrieved from 
http://www.ncbi.nlm.nih.gov/pubmed/11796152 
Kerns, J. G., Cohen, J. D., MacDonald, A. W., Cho, R. Y., Stenger, V. A., & Carter, C. S. (2004). Anterior 
cingulate conflict monitoring and adjustments in control. Science, 303(5660), 1023–6. 
doi:10.1126/science.1089910 
Khedr, E. M., Gamal, N. F. E., El-Fetoh, N. A., Khalifa, H., Ahmed, E. M., Ali, A. M., … Karim, A. A. (2014). 
A Double-Blind Randomized Clinical Trial on the Efficacy of Cortical Direct Current Stimulation for the 
Treatment of Alzheimer’s Disease. Frontiers in Aging Neuroscience, 6. 
https://doi.org/10.3389/fnagi.2014.00275 
Kim, M. J., & Whalen, P. J. (2009). The structural integrity of an amygdala-prefrontal pathway predicts trait 
anxiety. Journal Of Neuroscience, 29(37), 11614–8. doi:10.1523/JNEUROSCI.2335-09.2009 
Knott V, Bosman M, Mahoney C, Ilivitsky V, Quirt K (1999) Transdermal nicotine: single dose effects on mood, 
EEG, performance, and event-related potentials. Pharmacol Biochem Behav 63:253-261. 
 
Koob, G. F., & Volkow, N. D. (2010). Neurocircuitry of Addiction. Neuropsychopharmacology, 35(1), 217–
238. https://doi.org/10.1038/npp.2009.110 
Kozink, R. V, Kollins, S. H., & McClernon, F. J. (2010a). Smoking Withdrawal Modulates Right Inferior 
Frontal Cortex but not Presupplementary Motor Area Activation During Inhibitory Control. 
Neuropharmacology, 35(13), 2600–6. doi:10.1038/npp.2010.154 
Kozink, R. V, Kollins, S. H., & McClernon, F. J. (2010b). Smoking Withdrawal Modulates Right Inferior 
Frontal Cortex but not Presupplementary Motor Area Activation During Inhibitory Control. 
Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology, 
35(13), 2600–6. doi:10.1038/npp.2010.154 
Krebs, P., & Sherman, S. E. (2012). Review: Varenicline for tobacco cessation does not increase CV serious 
adverse events. Annals of Internal Medicine, 157(4), JC2–2. doi:10.7326/0003-4819-157-4-201208210-
02002 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Krueger, R. F., Markon, K. E., Patrick, C. J., Benning, S. D., & Kramer, M. D. (2007). Linking antisocial 
behavior, substance use, and personality: An integrative quantitative model of the adult externalizing 
spectrum. Journal of Abnormal Psychology, 116(4), 645. https://doi.org/10.1037/0021-843X.116.4.645 
Lanius, R. A., Bluhm, R. I., Coupland, N. J., Hegadoren, K. M., & Rowe, B. (2010). Default mode network 
connectivity as a predictor of post-traumatic stress disorder symptomseverityinacutelytraumatizedsubjects. 
Journal of Psychiatry & Neuroscience (JPN), 33–40. doi:10.1111/j.1600-0447.2009.01391.x 
Lerman C, Gu H, Loughead J, Ruparel K, Yang Y, & Stein EA. (2014). Large-scale brain network coupling 
predicts acute nicotine abstinence effects on craving and cognitive function. JAMA Psychiatry, 71(5), 523–
530. https://doi.org/10.1001/jamapsychiatry.2013.4091 
Leventhal, A. M., Ramsey, S. E., Brown, R. a, LaChance, H. R., & Kahler, C. W. (2008). Dimensions of 
depressive symptoms and smoking cessation. Nicotine & Tobacco Research : Official Journal of the 
Society for Research on Nicotine and Tobacco, 10(3), 507–17. doi:10.1080/14622200801901971 
Leventhal, A. M., Waters, A. J., Kahler, C. W., Ray, L. a, & Sussman, S. (2009). Relations between anhedonia 
and smoking motivation. Nicotine & Tobacco Research : Official Journal of the Society for Research on 
Nicotine and Tobacco, 11(9), 1047–54. doi:10.1093/ntr/ntp098 
Li, C.-S. R., Yan, P., Bergquist, K. L., & Sinha, R. (2007). Greater activation of the “default” brain regions 
predicts stop signal errors. NeuroImage, 38(3), 640–8. doi:10.1016/j.neuroimage.2007.07.021 
Li, Chiang-Shan, P., Chao, H. H., Sinha, R., Paliwal, P., Constable, R. T., Zhang, S., & Lee, T.-W. (2008). 
Error-specific medial cortical and subcortical activity during the stop signal task: a functional magnetic 
resonance imaging study. Neuroscience, 155(4), 1142–51. doi:10.1016/j.neuroscience.2008.06.062 
Loughead, J., Ray, R., Wileyto, E. P., Ruparel, K., Sanborn, P., Siegel, S., … Lerman, C. (2010). Effects of the 
alpha4beta2 partial agonist varenicline on brain activity and working memory in abstinent smokers. 
Biological Psychiatry, 67(8), 715–21. doi:10.1016/j.biopsych.2010.01.016 
Lu, H., & Stein, E. A. (2014). Resting state functional connectivity: Its physiological basis and application in 
neuropharmacology. Neuropharmacology, 84, 79–89. https://doi.org/10.1016/j.neuropharm.2013.08.023 
Lubin, J. H., Caporaso, N., Hatsukami, D. K., Joseph, A. M., & Hecht, S. S. (2007). The association of a 
tobacco-specific biomarker and cigarette consumption and its dependence on host characteristics. Cancer 
Epidemiology Biomarkers & Prevention, 16(9), 1852-1857. 
Luijten, M., van Meel, C. S., & Franken, I. H. a. (2011). Diminished error processing in smokers during 
smoking cue exposure. Pharmacology, Biochemistry, and Behavior, 97(3), 514–20. 
doi:10.1016/j.pbb.2010.10.012 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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MacKillop, J., & Kahler, C. W. (2009). Delayed reward discounting predicts treatment response for heavy 
drinkers receiving smoking cessation treatment. Drug and Alcohol Dependence, 104(3), 197–203. 
doi:10.1016/j.drugalcdep.2009.04.020 
Malarcher, A., Dube, S., Shaw, L., Babb, S., & Kaufmann, R. (2011). Quitting Smoking Among Adults - 
United States, 2001-2010. Morbity and Mortality Weekly Report, 60(44), 1513–1519. 
Mancuso G, Andres P, Ansseau M, Tirelli E (1999) Effects of nicotine administered via a transdermal delivery 
system on vigilance: a repeated measure study. Psychopharmacology (Berl) 142:18-23. 
Marcinkiewcz, C. a, Prado, M. M., Isaac, S. K., Marshall, A., Rylkova, D., & Bruijnzeel, A. W. (2009). 
Corticotropin-releasing factor within the central nucleus of the amygdala and the nucleus accumbens shell 
mediates the negative affective state of nicotine withdrawal in rats. Neuropharmacology, 34(7), 1743–52. 
doi:10.1038/npp.2008.231 
Marco-Pallarés, J., Camara, E., Münte, T. F., & Rodríguez-Fornells, A. (2008). Neural mechanisms underlying 
adaptive actions after slips. Journal of Cognitive Neuroscience, 20(9), 1595–610. 
doi:10.1162/jocn.2008.20117 
Markou, A. (2008). Review. Neurobiology of nicotine dependence. Philosophical Transactions of the Royal 
Society of London. Series B, Biological Sciences, 363(1507), 3159–68. doi:10.1098/rstb.2008.0095 
Marks, M., Stitzel, J., & Collins, A. (1986). Dose-response analysis of nicotine tolerance and receptor changes 
in two inbred mouse strains. J. Pharmacol. Exp. Ther., 239(2), 358–364. Retrieved from 
http://jpet.aspetjournals.org/cgi/content/abstract/239/2/358 
Marsh, A. A., Finger, E. C., Buzas, B., Soliman, N., Richell, R. A., Vythilingham, M., … Blair, R. J. R. (2006). 
Impaired recognition of fear facial expressions in 5-HTTLPR S-polymorphism carriers following 
tryptophan depletion. Psychopharmacology, 189(3), 387–94. doi:10.1007/s00213-006-0581-2 
McBride, D., Barrett, S. P., Kelly, J. T., Aw, A., & Dagher, A. (2006). Effects of expectancy and abstinence on 
the neural response to smoking cues in cigarette smokers: an fMRI study. Neuropharmacology, 31(12), 
2728–38. doi:10.1038/sj.npp.1301075 
McClernon, F. J., Kollins, S. H., Lutz, A. M., Fitzgerald, D. P., Murray, D. W., Redman, C., & Rose, J. E. 
(2008a). Effects of smoking abstinence on adult smokers with and without attention deficit hyperactivity 
disorder: results of a preliminary study. Psychopharmacology, 197(1), 95–105. doi:10.1007/s00213-007-
1009-3 
McClernon, F. J., Kollins, S. H., Lutz, A. M., Fitzgerald, D. P., Murray, D. W., Redman, C., & Rose, J. E. 
(2008b). Effects of smoking abstinence on adult smokers with and without attention deficit hyperactivity 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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disorder: results of a preliminary study. Psychopharmacology, 197(1), 95–105. doi:10.1007/s00213-007-
1009-3 
McClernon, F. J., Kozink, R. V, Lutz, A. M., & Rose, J. E. (2009a). 24-h smoking abstinence potentiates fMRI-
BOLD activation to smoking cues in cerebral cortex and dorsal striatum. Psychopharmacology, 204(1), 
25–35. doi:10.1007/s00213-008-1436-9 
McClernon, F. J., Kozink, R. V, Lutz, A. M., & Rose, J. E. (2009b, May). 24-h smoking abstinence potentiates 
fMRI-BOLD activation to smoking cues in cerebral cortex and dorsal striatum. Psychopharmacology. 
doi:10.1007/s00213-008-1436-9 
McNair D, Loor M, Droppleman L Profile of Mood States. In. San Diego, CA: Educational and Industrial Testing 
Service. 
Menon, V., & Uddin, L. Q. (2010). Saliency, switching, attention and control: a network model of insula 
function. Brain Structure & Function, 214(5-6), 655–67. doi:10.1007/s00429-010-0262-0 
Minnix, J. A., Robinson, J. D., Lam, C. Y., Carter, B. L., Foreman, J. E., Vandenbergh, D. J., … Cinciripini, P. 
M. (2011). The serotonin transporter gene and startle response during nicotine deprivation. Biological 
Psychology, 86(1), 1–8. doi:10.1016/j.biopsycho.2010.09.013 
Mitchell, S. (2004a). Effects of short-term nicotine deprivation on decision-making: Delay, uncertainty and 
effort discounting. Nicotine & Tobacco Research, 6(5), 819–828. doi:10.1080/14622200412331296002 
Mitchell, S. (2004b). Effects of short-term nicotine deprivation on decision-making: Delay, uncertainty and 
effort discounting. Nicotine & Tobacco Research, 6(5), 819–828. doi:10.1080/14622200412331296002 
Monk, C. S., Telzer, E. H., Mogg, K., Bradley, B. P., Mai, X., Louro, H. M. C., … Pine, D. S. (2008). 
Amygdala and ventrolateral prefrontal cortex activation to masked angry faces in children and adolescents 
with generalized anxiety disorder. Archives of General Psychiatry, 65(5), 568–76. 
doi:10.1001/archpsyc.65.5.568 
Myers, C. S., Taylor, R. C., Moolchan, E. T., & Heishman, S. J. (2008). Dose-related enhancement of mood and 
cognition in smokers administered nicotine nasal spray. Neuropharmacology, 33(3), 588–98. 
doi:10.1038/sj.npp.1301425 
Naqvi, N. H., & Bechara, A. (2009). The hidden island of addiction: the insula. Trends in Neurosciences, 32(1), 
56–67. doi:10.1016/j.tins.2008.09.009 
Naqvi, N. H., Rudrauf, D., Damasio, H., & Bechara, A. (2007). Damage to the insula disrupts addiction to 
cigarette smoking. Science, 315(5811), 531–4. doi:10.1126/science.1135926 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Nestler, E. J. (2005). Is there a common molecular pathway for addiction? Nature Neuroscience, 8(11), 1445–
1449. https://doi.org/10.1038/nn1578 
Ochsner, K. N., Hughes, B., Robertson, E. R., Cooper, J. C., & Gabrieli, J. D. E. (2010). Neural Systems 
Supporting the Control of Affective and Accessed Neural Systems Supporting the Control of Affective 
and Cognitive Conflicts. Journal of Cognitive Neuroscience. 
O’Connell, N. E., Cossar, J., Marston, L., Wand, B. M., Bunce, D., Moseley, G. L., & Souza, L. H. D. (2012). 
Rethinking Clinical Trials of Transcranial Direct Current Stimulation: Participant and Assessor Blinding 
Is Inadequate at Intensities of 2mA. PLOS ONE, 7(10), e47514. 
https://doi.org/10.1371/journal.pone.0047514 
Olive, M. F., Koenig, H. N., Nannini, M. a, & Hodge, C. W. (2002). Elevated extracellular CRF levels in the 
bed nucleus of the stria terminalis during ethanol withdrawal and reduction by subsequent ethanol intake. 
Pharmacology, Biochemistry, and Behavior, 72(1-2), 213–20. Retrieved from 
http://www.ncbi.nlm.nih.gov/pubmed/11900791 
Overbeek, T. J. M., Nieuwenhuis, S., & Ridderinkhof, K. R. (2005). Dissociable Components of Error 
Processing. Journal of Psychophysiology, 19(4), 319–329. doi:10.1027/0269-8803.19.4.319 
Pariyadath, V., Gowin, J. L., & Stein, E. A. (2015). Chapter 8 - Resting state functional connectivity analysis 
for addiction medicine: From individual loci to complex networks. In H. E. and M. P. Paulus (Ed.), 
Progress in Brain Research (Vol. 224, pp. 155–173). Elsevier. Retrieved from 
http://www.sciencedirect.com/science/article/pii/S0079612315001211 
Park, M.-S., Sohn, J.-H., Suk, J.-A., Kim, S.-H., Sohn, S., & Sparacio, R. (2007). Brain substrates of craving to 
alcohol cues in subjects with alcohol use disorder. Alcohol and Alcoholism (Oxford, Oxfordshire), 42(5), 
417–22. doi:10.1093/alcalc/agl117 
Perezstable, E. J., Benowitz, N. L., & Marin, G. (1995). Is Serum Cotinine a Better Measure of Cigarette-
Smoking Than Self-Report?. Preventive medicine, 24(2), 171-179. 
Peters, J., & Büchel, C. (2010). Episodic future thinking reduces reward delay discounting through an 
enhancement of prefrontal-mediotemporal interactions. Neuron, 66(1), 138–48. 
doi:10.1016/j.neuron.2010.03.026 
Pettiford, J., Kozink, R. V, Lutz, A. M., Kollins, S. H., Rose, J. E., & McClernon, F. J. (2007). Increases in 
impulsivity following smoking abstinence are related to baseline nicotine intake and boredom 
susceptibility. Addictive Behaviors, 32(10), 2351–7. doi:10.1016/j.addbeh.2007.02.004 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Pezawas, L., Meyer-Lindenberg, A., Drabant, E. M., Verchinski, B. a, Munoz, K. E., Kolachana, B. S., … 
Weinberger, D. R. (2005). 5-HTTLPR polymorphism impacts human cingulate-amygdala interactions: a 
genetic susceptibility mechanism for depression. Nature Neuroscience, 8(6), 828–34. doi:10.1038/nn1463 
Porjesz, B., Begleiter, H., Reich, T., Van Eerdewegh, P., Edenberg, H. J., Foroud, T., … Rohrbaugh, J. (1998). 
Amplitude of visual P3 event-related potential as a phenotypic marker for a predisposition to alcoholism: 
preliminary results from the COGA Project. Collaborative Study on the Genetics of Alcoholism. 
Alcoholism, Clinical and Experimental Research, 22(6), 1317–23. Retrieved from 
http://www.ncbi.nlm.nih.gov/pubmed/9756048 
Powell, J. H., Dawkins, L., & Davis, R. E. (2002). Inhibition : Tests of an Incentive Motivational Model. 
Biological Psychiatry. 
Powell, J. H., Dawkins, L., West, R., Powell, J., & Pickering, A. (2010). Relapse to smoking during unaided 
cessation: clinical, cognitive and motivational predictors. Psychopharmacology, 212(4), 537–49. 
doi:10.1007/s00213-010-1975-8 
Prochaska, J. O., Velicer, W. F., DiClemente, C. C., & Fava, J. (1988). Measuring processes of change: 
applications to the cessation of smoking. Journal of Consulting and Clinical Psychology, 56(4), 520–8. 
Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/3198809 
Pritchard W, Sokhadze E, Houlihan M (2004) Effects of nicotine and smoking on event-related potentials: a 
review. Nicotine Tob Res 6:961-984. 
 
Richardson, J. D. (2016, September). An Update on Blinding Practices in tDCS and tACS Research. Presented 
at the NIMH Transcranial Electrical Stimulation Workshop, Bethesda, MD. Retrieved from 
https://www.dropbox.com/s/1zmhr1x1b5zmreg/8%20Richardson%20Blinding.pdf?dl=0 
Russo, R., Wallace, D., Fitzgerald, P. B., & Cooper, N. R. (2013). Perception of Comfort During Active and 
Sham Transcranial Direct Current Stimulation: A Double Blind Study. Brain Stimulation, 6(6), 946–951. 
https://doi.org/10.1016/j.brs.2013.05.009 
Sakoğlu, U., Upadhyay, J., Chin, C.-L., Chandran, P., Baker, S. J., Cole, T. B., … Luo, F. (2011). Paradigm 
shift in translational neuroimaging of CNS disorders. Biochemical Pharmacology, 81(12), 1374–87. 
doi:10.1016/j.bcp.2010.12.029 
Schacter, D. L., Addis, D. R., & Buckner, R. L. (2007). Remembering the past to imagine the future: the 
prospective brain. Nature Reviews Neuroscience, 8(9), 657–61. doi:10.1038/nrn2213 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Seeley, W. W., Menon, V., Schatzberg, A. F., Keller, J., Glover, G. H., Kenna, H., … Greicius, M. D. (2007). 
Dissociable intrinsic connectivity networks for salience processing and executive control. Journal Of 
Neuroscience, 27(9), 2349–56. doi:10.1523/JNEUROSCI.5587-06.2007 
Sheline, Y. I., Price, J. L., Yan, Z., & Mintun, M. a. (2010). Resting-state functional MRI in depression 
unmasks increased connectivity between networks via the dorsal nexus. Proceedings Of The National 
Academy Of Sciences Of The United States Of America, 107(24), 11020–5. doi:10.1073/pnas.1000446107 
Singleton, E. G., Anderson, L. M., & Heishman, S. J. (2003). Reliability and validity of the Tobacco Craving 
Questionnaire and validation of a craving-induction procedure using multiple measures of craving and 
mood. Addiction (Abingdon, England), 98(11), 1537–46. Retrieved from 
http://www.ncbi.nlm.nih.gov/pubmed/14616180 
Sinha, R., & Li, C. S. R. (2007). Imaging stress- and cue-induced drug and alcohol craving: association with 
relapse and clinical implications. Drug and Alcohol Review, 26(1), 25–31. 
doi:10.1080/09595230601036960 
Snaith, R. P., Hamilton, M., Morley, S., Humayan, A., Hargreaves, D., & Trigwell, P. (1995). A scale for the 
assessment of hedonic tone the Snaith-Hamilton Pleasure Scale. The British Journal of Psychiatry, 167(1), 
99–103. doi:10.1192/bjp.167.1.99 
Spielberger, C. D. (1983). Manual for the State-Trait Anxiety Inventory (STAI-form Y). Palo Alto: Consulting 
Psychologists Press. 
Sridharan, D., Levitin, D. J., & Menon, V. (2008). A critical role for the right fronto-insular cortex in switching 
between central-executive and default-mode networks. Proceedings Of The National Academy Of Sciences 
Of The United States Of America, 105(34), 12569–74. doi:10.1073/pnas.0800005105 
Sutherland, M. T., McHugh, M. J., Pariyadath, V., & Stein, E. A. (2012). Resting state functional connectivity 
in addiction: Lessons learned and a road ahead. NeuroImage, 62(4), 2281–2295. 
https://doi.org/10.1016/j.neuroimage.2012.01.117 
Sutherland, M. T., Yanes, J. A., & Stein, E. A. (2017). Chapter 18 - Neuroimaging Insights Into the 
Multifaceted Nature of the Nicotine Withdrawal Syndrome. In Negative Affective States and Cognitive 
Impairments in Nicotine Dependence (pp. 311–330). San Diego: Academic Press. Retrieved from 
http://www.sciencedirect.com/science/article/pii/B9780128025741000181 
Swan, G. E., McClure, J. B., Jack, L. M., Zbikowski, S. M., Javitz, H. S., Catz, S. L., … McAfee, T. a. (2010a). 
Behavioral counseling and varenicline treatment for smoking cessation. American Journal of Preventive 
Medicine, 38(5), 482–90. doi:10.1016/j.amepre.2010.01.024 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Swan, G. E., McClure, J. B., Jack, L. M., Zbikowski, S. M., Javitz, H. S., Catz, S. L., … McAfee, T. A. 
(2010b). Behavioral counseling and varenicline treatment for smoking cessation. American Journal of 
Preventive Medicine, 38(5), 482–90. doi:10.1016/j.amepre.2010.01.024 
Sweet, L. H., Mulligan, R. C., Finnerty, C. E., Jerskey, B. A., David, S. P., Cohen, R. A., & Niaura, R. S. 
(2010). Effects of nicotine withdrawal on verbal working memory and associated brain response. 
Psychiatry Research, 183(1), 69–74. doi:10.1016/j.pscychresns.2010.04.014 
Thakur, G. A., Grizenko, N., Sengupta, S. M., Schmitz, N., & Joober, R. (2010). The 5-HTTLPR polymorphism 
of the serotonin transporter gene and short term behavioral response to methylphenidate in children with 
ADHD. BMC Psychiatry, 10(1), 50. doi:10.1186/1471-244X-10-50 
Tsai, S.-T., Cho, H.-J., Cheng, H.-S., Kim, C.-H., Hsueh, K.-C., Billing, C. B., & Williams, K. E. (2007). A 
randomized, placebo-controlled trial of varenicline, a selective alpha4beta2 nicotinic acetylcholine 
receptor partial agonist, as a new therapy for smoking cessation in Asian smokers. Clinical Therapeutics, 
29(6), 1027–39. doi:10.1016/j.clinthera.2007.06.011 
Turk-Browne, N. B. (2013). Functional Interactions as Big Data in the Human Brain. Science, 342(6158), 580–
584. https://doi.org/10.1126/science.1238409 
Uhl, G. R., Liu, Q.-R., Drgon, T., Johnson, C., Walther, D., Rose, J. E., … Lerman, C. (2008). Molecular 
genetics of successful smoking cessation: convergent genome-wide association study results. Archives of 
General Psychiatry, 65(6), 683–93. doi:10.1001/archpsyc.65.6.683 
Ullsperger, M., Harsay, H. a, Wessel, J. R., & Ridderinkhof, K. R. (2010). Conscious perception of errors and 
its relation to the anterior insula. Brain Structure & Function, 214(5-6), 629–43. doi:10.1007/s00429-010-
0261-1 
VanderVeen, J. W., Cohen, L. M., Cukrowicz, K. C., & Trotter, D. R. M. (2008). The role of impulsivity on 
smoking maintenance. Nicotine & Tobacco Research : Official Journal of the Society for Research on 
Nicotine and Tobacco, 10(8), 1397–404. doi:10.1080/14622200802239330 
Velicer, W. F., DiClemente, C. C., Prochaska, J. O., & Brandenburg, N. (1985). Decisional balance measure for 
assessing and predicting smoking status. Journal of Personality and Social Psychology, 48(5), 1279–89. 
Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/3998990 
Velicer, W. F., Diclemente, C. C., Rossi, J. S., & Prochaska, J. O. (1990). Relapse situations and self-efficacy: 
an integrative model. Addictive Behaviors, 15(3), 271–83. Retrieved from 
http://www.ncbi.nlm.nih.gov/pubmed/2378287 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Versace, F., Lam, C. Y., Engelmann, J. M., Robinson, J. D., Minnix, J. A., Brown, V. L., & Cinciripini, P. M. 
(2011). Beyond cue reactivity: blunted brain responses to pleasant stimuli predict long-term smoking 
abstinence. Addiction Biology. doi:10.1111/j.1369-1600.2011.00372.x 
Wang, Z., Faith, M., Patterson, F., Tang, K., Kerrin, K., Wileyto, E. P., … Lerman, C. (2007). Neural substrates 
of abstinence-induced cigarette cravings in chronic smokers. Journal Of Neuroscience, 27(51), 14035–40. 
doi:10.1523/JNEUROSCI.2966-07.2007 
Wang, Z., Ray, R., Faith, M., Tang, K., Wileyto, E. P., Detre, J. A., & Lerman, C. (2008). Nicotine abstinence-
induced cerebral blood flow changes by genotype. Neuroscience Letters, 438(3), 275–80. 
doi:10.1016/j.neulet.2008.04.084 
Watson, D., Clark, L. A., & Tellegen, A. (1988). Development and validation of brief measures of positive and 
negative affect: The PANAS scales. Journal of Personality and Social Psychology, 54(6), 1063–1070. 
Retrieved from http://psycnet.apa.org/journals/psp/54/6/1063 
Weinstein, a, Greif, J., Yemini, Z., Lerman, H., Weizman, a, & Even-Sapir, E. (2010). Attenuation of cue-
induced smoking urges and brain reward activity in smokers treated successfully with bupropion. Journal 
of Psychopharmacology (Oxford, England), 24(6), 829–38. doi:10.1177/0269881109105456 
Wertz, J. M., & Sayette, M. A. (2001). A review of the effects of perceived drug use opportunity of self-
reported urge. Experimental and Clinical Psychopharmacology, 9(1), 3–13. Retrieved from 
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2632967&tool=pmcentrez&rendertype=abstra
ct 
Wilcox, C. E., Teshiba, T. M., Merideth, F., Ling, J., & Mayer, A. R. (2011). Enhanced cue reactivity and 
fronto-striatal functional connectivity in cocaine use disorders. Drug and Alcohol Dependence, 115(1-2), 
137–44. doi:10.1016/j.drugalcdep.2011.01.009 
Wilson, S. J., Sayette, M. a, & Fiez, J. a. (2004a). Prefrontal responses to drug cues: a neurocognitive analysis. 
Nature Neuroscience, 7(3), 211–4. doi:10.1038/nn1200 
Wilson, S. J., Sayette, M. A., & Fiez, J. A. (2004b). Prefrontal responses to drug cues: a neurocognitive 
analysis. Nature Neuroscience, 7(3), 211–4. doi:10.1038/nn1200 
Wilson, S. J., Sayette, M. A., & Fiez, J. A. (2012). Quitting-unmotivated and quitting-motivated cigarette 
smokers exhibit different patterns of cue-elicited brain activation when anticipating an opportunity to 
smoke. Journal of Abnormal Psychology, 121(1), 198–211. doi:10.1037/a0025112 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
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Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Woods, A. J., Antal, A., Bikson, M., Boggio, P. S., Brunoni, A. R., Celnik, P., … Nitsche, M. A. (2016). A 
technical guide to tDCS, and related non-invasive brain stimulation tools. Clinical Neurophysiology, 
127(2), 1031–1048. https://doi.org/10.1016/j.clinph.2015.11.012 
Wörsching, J., Padberg, F., Ertl-Wagner, B., Kumpf, U., Kirsch, B., & Keeser, D. (2016). Imaging transcranial 
direct current stimulation (tDCS) of the prefrontal cortex—correlation or causality in stimulation-mediated 
effects? Neuroscience & Biobehavioral Reviews, 69, 333–356. 
https://doi.org/10.1016/j.neubiorev.2016.08.001 
Xu J, Mendrek A, Cohen MS, Monterosso J, Rodriguez P, Simon SL, Brody A, Jarvik M, Domier CP, Olmstead 
R, Ernst M, London ED (2005) Brain activity in cigarette smokers performing a working memory task: 
effect of smoking abstinence. Biol Psychiatry 58:143-150. 
 
Yeo, B. T. T., Krienen, F. M., Sepulcre, J., Sabuncu, M. R., Lashkari, D., Hollinshead, M., … Buckner, R. L. 
(2011). The organization of the human cerebral cortex estimated by intrinsic functional connectivity. 
Journal of Neurophysiology, 106(3), 1125–1165. https://doi.org/10.1152/jn.00338.2011 
Yoon, J. H., Higgins, S. T., Heil, S. H., Sugarbaker, R. J., Thomas, C. S., & Badger, G. J. (2007). Delay 
discounting predicts postpartum relapse to cigarette smoking among pregnant women. Experimental and 
Clinical Psychopharmacology, 15(2), 176–86. doi:10.1037/1064-1297.15.2.186 
Zevin, S., Jacob, P., & Benowitz, N. L. (1998). Dose-related cardiovascular and endocrine effects of 
transdermal nicotine. Clinical Pharmacology and Therapeutics, 64(1), 87–95. doi:10.1016/S0009-
9236(98)90026-1 
Zhou, Y., Yu, C., Zheng, H., Liu, Y., Song, M., Qin, W., … Jiang, T. (2010). Increased neural resources 
recruitment in the intrinsic organization in major depression. Journal of Affective Disorders, 121(3), 220–
30. doi:10.1016/j.jad.2009.05.029 
Zuckerman M, Eysenck S, Eysenck HJ (1978) Sensation seeking in England and America: cross-cultural, age, 
and sex comparisons. J Consult Clin Psychol 46:139-149. 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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24.  Appendix 1: Participant Eligibility Checklists 
 
Eligibility Checklist for Protocol 474-Nontreatment 
 
Screening  
 
Inclusion Criteria  
1. Be between the ages of 18-55. Be right-handed. Assessment tool(s): Edinburgh Handedness Inventory.  
(YES/NO) 
2. Be free of active DSM-IV dependence, or dependence in partial remission, on alcohol or any drug except 
nicotine.  Past active dependence is acceptable provided it is at least five years in the past and total time of 
active dependence did not exceed 4 years. Those with past dependence on any substance other than alcohol 
or marijuana may not have any current use (past 6 months) of the substance on which they were 
dependent. For individuals with past alcohol or marijuana dependence, current use of the previously 
dependent substance will be allowed providing they do not meet any current DSM-IV criteria for 
substance dependence, with the exception of tolerance.   Assessment tool(s): The computerized SCID and 
clinical substance abuse/dependence assessment. While recreational/intermittent use of alcohol and/or 
marijuana will be tolerated in all participant groups, individuals will be excluded if they meet current or 
recent (within 5 years) DSM-IV diagnostic criteria for dependence on any substances.  
(YES/NO) 
3. Be able to abstain from alcohol 24hrs before each of the imaging sessions and able to moderate their 
caffeine intake 12hrs before each session Assessment tool: Direct question and documented response 
(YES/NO) 
4. Must have a urine cotinine  level corresponding to smoker status for the specific test being used, typically 
corresponding to a urine cotinine above about 200 ng/ml, and have been smoking consistently for at least 
one year. For lighter smokers (less than 10 cpd), this is defined as smoking at their current level or more for 
at least the past year (excluding any quit attempts in the last year). For heavier smokers (more than 10 cpd), 
they must have been smoking at least an average of 10 cpd for at least the past year (excluding quit attempts). 
Assessment tool(s): Self-report, commercial urine cotinine test corresponding to smoker status for the 
specific test being used, typically corresponding to a urine cotinine above about 200 ng/ml. 
 (YES/NO) 
5. Be able to abstain from smoking for 36hrs on two occasions during the study.  Assessment tool: Direct 
question and documented response 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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(YES/NO) 
6. Be comfortable with the scanning requirements of the study  
Prospective participants will be questioned about symptoms of claustrophobia and placed in the 
mock scanner during their first visit to assess for possible difficulty tolerating the confinement of the 
scanner and for ability to fit into the scanner. Assessment tool: mock Scanner Trial 
(YES/NO) 
7. Be English-speaking. Assessment tool: self-report. 
(YES/NO) 
Medical 
Inclusion criteria  
1. Be in good health. Assessment tool(s): Participants will provide a brief health history during phone 
screening, and undergo a medical history and physical examination with a qualified IRP clinician.  
(YES/NO) 
Exclusion criteria 
1. Are cognitively impaired or learning disabled. Assessment tool(s): History of placement in special-
education classes as a consequence of serious learning problems and not solely as a consequence of 
behavioral problems, assessed during the History and Physical screening assessment. 
 (YES/NO) 
2. are not suitable to undergo an fMRI experiment due to certain implanted devices (cardiac pacemaker or 
neurostimulator, some artificial joints, metal pins, surgical clips or other implanted metal parts), body 
morphology, or claustrophobia. Assessment tool(s): Prospective participants will fill out an MRI 
screening questionnaire and undergo an interview with an MR technologist.  Questions concerning 
suitability for scanning will be referred to the MR Medical Safety Officer.  
(YES/NO) 
3. have coagulopathies, history of, current superficial, or deep vein thrombosis, musculoskeletal 
abnormalities restricting an individual’s ability to lie flat for extended periods of time. Assessment 
tool(s): History and physical examination by a qualified IRP clinician, supplemented with a trial of 
lying in the mock scanner to assess comfort issues.  
(YES/NO) 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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4. have HIV or Syphilis. Assessment tool(s): Oral HIV followed by blood test if oral test is + and STS+ 
without adequate prior treatment. 
(YES/NO) 
5. regularly use any prescription (e.g., antidepressants, benzodiazepines, antipsychotics, anticonvulsants, 
barbiturates), over-the-counter (e.g., cold medicine) or herbal medication (e.g., Kava, Gingko biloba, St. 
John’s wort) that may alter CNS function, cardiovascular function, or neuronal-vascular coupling. 
Assessment tool(s): History and comprehensive urine drug screening to detect antidepressants, 
benzodiazepines, antipsychotics, anticonvulsants, and barbiturates.  
(YES/NO) 
6. have any current neurological illnesses including, but not limited to, seizure disorders, frequent migraines 
or on prophylaxis, multiple sclerosis, movement disorders, history of significant head trauma, or CNS 
tumor. Assessment tool(s):  History and physical examination by a qualified IRP clinician, urine drug 
screening for anticonvulsants not disclosed by history. History of head trauma with loss of consciousness 
of more than 30 minutes or with post-concussive sequelae lasting more than two days, regardless of loss 
of consciousness, will be exclusionary. The MAI will also retain discretion to exclude based on a history 
of neurological illness that may compromise data integrity.  
(YES/NO) 
7. Have any current major psychiatric disorders to include, but not limited to, mood, anxiety, psychotic 
disorders, or substance-induced psychiatric disorders, or any current suicidal ideations or history of 
suicide attempts or currently under antidepressant or antipsychotic medication treatment. The MAI will 
reserve the right to exclude on the basis of psychiatric history not explicitly described in this criterion 
Assessment tool(s): Computerized SCID-NP, ASRS (adult ADHD self-report scale), Beck Depression 
Inventory, Beck Anxiety Inventory, and DSM-IV (DSM-IV, APA, 1994) confirmed by clinician 
interview. 
(YES/NO) 
8. have significant cardiovascular or cerebrovascular conditions. Assessment tool(s): History and physical 
exam, including 12-lead EKG. 
(YES/NO) 
9. have any other major medical condition that in the view of the investigators would compromise the 
safety of an individual during participation**. Assessment tool(s): History and physical examination by 
a qualified IRP clinician and CBC, urinalysis, NIDA chemistry panel (liver function tests, electrolytes, 
kidney function). The following lab values will result in exclusion from the study:  
  
(a) Hemoglobin < 10 g/dl  
 
 
(YES/NO) 
(b) White Blood Cell Count < 2400/µl   
(YES/NO) 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 128 of 221 
 
 
(c) Liver Function Tests > 3X normal   
(YES/NO) 
(d) Serum glucose > 200 mg/dl   
 
(YES/NO) 
(e) Urine protein > 2+   
 
 
(YES/NO) 
(f) Serum creatinine > 2 mg/dl   
 
(YES/NO) 
(g) Estimated creatinine clearance <60ml/min  (YES/NO) 
 (YES/NO) 
**The MAI will retain discretion to exclude based on less extreme lab results.  After the 
screening process has been completed, the MAI will take into account all data collected in order to 
decide if there is an existing medical illness that would compromise participation in this research.  
10. pregnant, planning to become pregnant, or breastfeeding.  Females are instructed in the consent to use 
effective forms of birth control during the study period.  Assessment tool(s): Urine and/or serum 
pregnancy tests, and clinical interview.    
(YES/NO) 
 
 
Eligibility Checklist for Protocol 474-treatment 
 
Screening  
 
Inclusion Criteria  
1. Be between the ages of 18-55. Be right-handed. Assessment tool(s): Edinburgh Handedness Inventory.  
(YES/NO) 
2. Be free of active DSM-IV dependence, or dependence in partial remission, on alcohol or any drug except 
nicotine.  Past active dependence is acceptable provided it is at least five years in the past and total time of 
active dependence did not exceed 4 years. Those with past dependence on any substance other than alcohol 
or marijuana may not have any current use (past 6 months) of the substance on which they were dependent. 
For individuals with past alcohol or marijuana dependence, current use of the previously dependent 
substance will be allowed providing they do not meet any current DSM-IV criteria for substance dependence, 
with the exception of tolerance.   Assessment tool(s): The computerized SCID and clinical substance 
abuse/dependence assessment. While recreational/intermittent use of alcohol and/or marijuana will be 
tolerated in all participant groups, individuals will be excluded if they meet current or recent (within 5 years) 
DSM-IV diagnostic criteria for dependence on any substances.  
(YES/NO) 
3. Be able to abstain from alcohol 24hrs before each of the imaging sessions and able to moderate their caffeine 
intake 12hrs before each session Assessment tool: Direct question and documented response 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 129 of 221 
 
 
(YES/NO) 
4. Must have a urine cotinine level corresponding to smoker status for the specific test being used, typically 
corresponding to a urine cotinine above about 200 ng/ml, and have been smoking consistently for at least 
one year. For lighter smokers (less than 10 cpd), this is defined as smoking at their current level or more for 
at least the past year (excluding any quit attempts in the last year). For heavier smokers (more than 10 cpd), 
they must have been smoking at least an average of 10 cpd for at least the past year (excluding quit attempts). 
Assessment tool(s): Self-report, commercial urine cotinine test corresponding to smoker status for the 
specific test being used, typically corresponding to a urine cotinine above about 200 ng/ml. 
 (YES/NO) 
5. Be able to abstain from smoking for 36hrs on two occasions during the study.  Assessment tool: Direct 
question and documented response 
(YES/NO) 
6. Be comfortable with the scanning requirements of the study  
Prospective participants will be questioned about symptoms of claustrophobia and placed in the 
mock scanner during their first visit to assess for possible difficulty tolerating the confinement of the 
scanner and for ability to fit into the scanner. Assessment tool: mock Scanner Trial 
(YES/NO) 
7. Be actively seeking treatment for smoking cessation and willing to engage in 12-weeks of treatment 
involving daily administration of Varenicline and weekly counseling sessions, as well as follow-up 
assessments at 1, 6 and 12 months following treatment onset. Assessment tool: Treatment motivation 
screening tool as well as direct question and documented response 
(YES/NO) 
8. Be English-speaking. Assessment tool: self-report. 
(YES/NO) 
Medical 
Inclusion criteria  
1. Be in good health. Assessment tool(s): Participants will provide a brief health history during phone 
screening, and undergo a medical history and physical examination with a qualified IRP clinician.  
(YES/NO) 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 130 of 221 
 
 
Exclusion criteria 
1. Are cognitively impaired or learning disabled. Assessment tool(s): History of placement in special-
education classes as a consequence of serious learning problems and not solely as a consequence of 
behavioral problems, assessed during the History and Physical screening assessment. 
 (YES/NO) 
2. are not suitable to undergo an fMRI experiment due to certain implanted devices (cardiac pacemaker or 
neurostimulator, some artificial joints, metal pins, surgical clips or other implanted metal parts), body 
morphology, or claustrophobia. Assessment tool(s): Prospective participants will fill out an MRI 
screening questionnaire and undergo an interview with an MR technologist.  Questions concerning 
suitability for scanning will be referred to the MR Medical Safety Officer.  
(YES/NO) 
3. have coagulopathies, history of, current superficial, or deep vein thrombosis, musculoskeletal 
abnormalities restricting an individual’s ability to lie flat for extended periods of time. Assessment 
tool(s): History and physical examination by a qualified IRP clinician, supplemented with a trial of 
lying in the mock scanner to assess comfort issues.  
(YES/NO) 
4. have HIV or Syphilis. Assessment tool(s): Oral HIV followed by blood test if oral test is + and STS+ 
without adequate prior treatment. 
(YES/NO) 
5. regularly use any prescription (e.g., antidepressants, benzodiazepines, antipsychotics, anticonvulsants, 
barbiturates), over-the-counter (e.g., cold medicine) or herbal medication (e.g., Kava, Gingko biloba, St. 
John’s wort) that may alter CNS function, cardiovascular function, or neuronal-vascular coupling. 
Assessment tool(s): History and comprehensive urine drug screening to detect antidepressants, 
benzodiazepines, antipsychotics, anticonvulsants, and barbiturates.  
(YES/NO) 
6. have any current neurological illnesses including, but not limited to, seizure disorders, frequent migraines 
or on prophylaxis, multiple sclerosis, movement disorders, history of significant head trauma, or CNS 
tumor. Assessment tool(s):  History and physical examination by a qualified IRP clinician, urine drug 
screening for anticonvulsants not disclosed by history. History of head trauma with loss of consciousness 
of more than 30 minutes or with post-concussive sequelae lasting more than two days, regardless of loss 
of consciousness, will be exclusionary. The MAI will also retain discretion to exclude based on a history 
of neurological illness that may compromise data integrity.  
(YES/NO) 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 131 of 221 
 
 
7. Have any current major psychiatric disorders to include, but not limited to, mood, anxiety, psychotic 
disorders, or substance-induced psychiatric disorders, or any current suicidal ideations or history of 
suicide attempts or currently under antidepressant or antipsychotic medication treatment. The MAI will 
reserve the right to exclude on the basis of psychiatric history not explicitly described in this criterion 
Assessment tool(s): Computerized SCID-NP, ASRS (adult ADHD self-report scale), Beck Depression 
Inventory, Beck Anxiety Inventory, and DSM-IV (DSM-IV, APA, 1994) confirmed by clinician 
interview. 
(YES/NO) 
8. have significant cardiovascular or cerebrovascular conditions. Assessment tool(s): History and physical 
exam, including 12-lead EKG. 
(YES/NO) 
9. have any other major medical condition that in the view of the investigators would compromise the 
safety of an individual during participation**. Assessment tool(s): History and physical examination by 
a qualified IRP clinician and CBC, urinalysis, NIDA chemistry panel (liver function tests, electrolytes, 
kidney function). The following lab values will result in exclusion from the study:  
  
(h) Hemoglobin < 10 g/dl  
 
 
(YES/NO) 
(i) White Blood Cell Count < 2400/µl   
(YES/NO) 
(j) Liver Function Tests > 3X normal   
(YES/NO) 
(k) Serum glucose > 200 mg/dl   
 
(YES/NO) 
(l) Urine protein > 2+   
 
 
(YES/NO) 
(m) Serum creatinine > 2 mg/dl   
 
(YES/NO) 
(n) Estimated glomerular filtration rate <60ml/min  
(YES/NO) 
 (YES/NO) 
**The MAI will retain discretion to exclude based on less extreme lab results.  After the 
screening process has been completed, the MAI will take into account all data collected in order to 
decide if there is an existing medical illness that would compromise participation in this research.  
10. pregnant, planning to become pregnant, or breastfeeding.  Females are instructed in the consent to use 
effective forms of birth control during the study period.  Assessment tool(s): Urine and/or serum 
pregnancy tests, and clinical interview.    
(YES/NO) 
11. have moderate to severe renal impairment. Assessment tool(s): Estimated glomerular filtration rate. Renal 
insufficiency with estimated creatinine clearance < 60 ml/min calculated by the Cockcroft-Gault equation 
will be excluded. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 132 of 221 
 
 
(YES/NO) 
12. Are diabetic.  Assessment tool(s): Casual plasma glucose testing.  Individuals with glucose levels above 
200 mg/dl may be further evaluated for diabetes using a fasting glucose test or be excluded. 
(YES/NO) 
 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 133 of 221 
 
 
Eligibility Checklist for Protocol 474-tDCS – Main Arm  
 
Screening 
 
All participants 
 
 
Yes 
No 
MAI 
Assessment Tool(s) 
1. Age 18-65 years. 
 
 
 
 
2. Right handed. 
 
 
 
Edinburg Handedness Inventory 
3. Free of current moderate-severe DSM-V 
Substance Use Disorder on any drug, except 
nicotine in smokers. If there is past moderate to 
severe substance dependence, must currently be in 
sustained remission (and not on maintenance 
therapy for opioid use disorder) and not 
intoxicated on the day of the imaging session. 
 
 
 
 
Computerized SCID 
DSM-5 SUD Assessment 
Clinical Assessment 
Urine Toxicology Screen 
Breathalyzer 
4. Able to abstain from alcohol and other 
recreational drugs for 24 hours before each 
imaging session, and able to moderate caffeine 
intake 12 hours before each imaging session. 
 
 
 
 
Self report 
5. English-speaking 
 
 
 
Self report 
 
Smoker Group 
 
 
Yes 
No 
MAI 
Assessment Tool(s) 
6. Have a urine cotinine equivalent of about 
200ng/ml or higher and have been smoking for at 
least 1 year. 
 
 
 
Self report 
Urine cotinine test 
7. Able to abstain from smoking for 12 hours prior to 
MRI-tDCS study sessions. 
 
 
 
Self-report and expired CO levels 
 
 
Non-smoker Group 
 
 
Yes 
No 
MAI 
Assessment Tool(s) 
6. Not have a history of daily cigarette smoking or 
have used any nicotine products continuously 
lasting more than a month, and no smoking or 
continuous use of any nicotine products within the 
past year. 
 
 
 
Self report 
 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 134 of 221 
 
 
Medical  
All participants 
 
 
 
Yes 
No 
MAI 
Assessment Tool(s) 
1.  
Be in good health. 
 
 
 
Health history, H&P 
 
2.  
Are suitable for MRI scanning  
 
(i.e. Free of certain implanted devices 
(cardiac pacemaker or neurostimulator, some 
artificial joints, metal pins, surgical clips or 
other implanted metal parts), have 
appropriate body morphology, free of 
claustrophobia.) 
 
 
 
 
MRI screening questionnaire and 
interview by MR technologist. 
 
Questions concerning suitability 
referred to MR Medical Safety 
Officer. 
 
3.  
Free of musculoskeletal abnormalities 
restricting an individual’s ability to lie flat 
for extended periods of time. 
 
 
 
 
H&P 
Mock scanner trial 
4.  
Free of HIV or Syphilis 
 
 
 
Oral HIV, Blood test if (+) Oral 
(+) STS (w/o Prior Tx) 
5.  
Does NOT regularly use any prescription 
(e.g., benzodiazepines, antipsychotics, 
anticonvulsants, barbiturates), over-the-
counter (e.g., cold medicine) or herbal 
medication (e.g., Kava, Gingko biloba, St. 
John’s wort) that may alter neuronal-
vascular coupling, with some exceptions 
below: 
 
May use anti-depressant medications. 
May use certain medications intermittently, 
provided the participant can be scanned in 
the same medication state across multiple 
study days for data consistency (i.e. either all 
scan days are scheduled after 5 half-lives 
since last medication use; or all scan days are 
scheduled on medication). 
 
 
 
 
History 
 
Comprehensive Urine Drug 
Screen 
6.  
Free of current neurological illnesses 
including, but not limited to, seizure 
disorders, frequent migraines or on 
prophylaxis, multiple sclerosis, movement 
disorders (except essential tremor, so long as 
 
 
 
H & P 
 
Comprehensive Urine Drug 
Screen 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 135 of 221 
 
 
it would not interfere with study tasks such 
as button pressing), cerebrovascular 
accident, history of significant head trauma, 
or CNS tumor.   
 
History of head trauma (loss of 
consciousness > 30 minutes OR  
sequelae lasting >2 days)  
7.  
Free of current major psychotic disorders, 
mania, substance-induced psychiatric 
disorders, or any current suicidal ideations or 
history of suicide attempts. Free of moderate 
to severe current symptoms of mood or 
anxiety disorders. 
 
(Mild mood or anxiety disorder symptoms 
are not exclusionary, whether medicated or 
unmedicated.) 
 
 
 
 
 
Computerized SCID,  
Clinician interview 
8.  
NOT cognitively impaired or learning 
disabled. 
 
 
 
H&P Screening (history of 
placement in special education 
classes as a consequence of 
serious learning problems, and not 
solely as a consequence of 
behavioral problems) 
 
9.  
Free of significant cardiovascular conditions 
that would make use of nicotine patch 
unsafe. 
 
 
 
 
H&P, 12-lead EKG 
10.  
Free of any other major medical condition 
that in the view of the investigators would 
compromise the safety of an individual 
during participation, or the quality of the 
data obtainable, including but not limited to 
lab values outside the following parameters:  
 
Hemoglobin < 10 g/dl  
White Blood Cell Count < 2400/µl  
Liver Function Tests > 3X upper limit of 
normal 
Serum glucose > 200 mg/dl  
Urine protein > 2+ 
Serum creatinine > 2 mg/dl 
Must be free of Diabetes Mellitus 
 
 
 
 
H&P, CBC, urinalysis, NIDA 
chemistry panel (liver function 
tests, electrolytes, kidney 
function).  
 
The MAI will retain discretion to 
exclude based on less extreme lab 
results, or other major medical 
condition not otherwise specified 
11.  
Is NOT pregnant, planning to become 
pregnant, or breastfeeding (females). 
 
 
 
Urine and/or serum pregnancy 
tests,  

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 136 of 221 
 
 
 
 
Clinical interview.   
 
Females are instructed in the 
consent to use effective forms of 
birth control during the study 
period.   
 
12.  
Has NOT participated in any brain 
stimulation session less than two weeks ago, 
or undergone brain stimulation exposure for 
treatment purposes in the last 6 months, 
including tDCS or transcranial magnetic 
stimulation (TMS). 
 
 
 
H&P, Clinical interview 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 137 of 221 
 
 
Eligibility Checklist for Protocol 474-tDCS – Pilot 
Screening 
 
 
 
 
Yes 
No 
MAI 
Assessment Tool(s) 
1. Age 18-65 years. 
 
 
 
 
2. Right handed. 
 
 
 
Edinburg Handedness Inventory 
3. Free of current moderate-severe DSM-V 
Substance Use Disorder on any drug, except 
nicotine in smokers. If there is past moderate to 
severe substance dependence, must currently be in 
sustained remission (and not on maintenance 
therapy for opioid use disorder) and not 
intoxicated on the day of the imaging session. 
 
 
 
 
Computerized SCID 
DSM-5 SUD Assessment 
Clinical Assessment 
Urine Toxicology Screen 
Breathalyzer 
4. Able to abstain from alcohol and other 
recreational drugs for 24 hours before each 
imaging session, and able to moderate caffeine 
intake 12 hours before each imaging session. 
 
 
 
 
Self report 
5. Not have a history of daily cigarette smoking or 
have used any nicotine products continuously 
lasting more than a month, and no smoking or 
continuous use of any nicotine products within the 
past year. 
 
 
 
Self report 
 
6. English-speaking 
 
 
 
Self report 
 
 
 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 138 of 221 
 
 
Medical  
 
 
 
Yes 
No 
MAI 
Assessment Tool(s) 
1.  
Be in good health. 
 
 
 
Health history, H&P 
 
2.  
Are suitable for MRI scanning  
 
(i.e. Free of certain implanted devices 
(cardiac pacemaker or neurostimulator, some 
artificial joints, metal pins, surgical clips or 
other implanted metal parts), have 
appropriate body morphology, free of 
claustrophobia.) 
 
 
 
 
MRI screening questionnaire and 
interview by MR technologist. 
 
Questions concerning suitability 
referred to MR Medical Safety 
Officer. 
 
3.  
Free of musculoskeletal abnormalities 
restricting an individual’s ability to lie flat 
for extended periods of time. 
 
 
 
 
H&P 
Mock scanner trial 
4.  
Free of HIV or Syphilis 
 
 
 
Oral HIV, Blood test if (+) Oral 
(+) STS (w/o Prior Tx) 
5.  
Does NOT regularly use any prescription 
(e.g., benzodiazepines, antipsychotics, 
anticonvulsants, barbiturates), over-the-
counter (e.g., cold medicine) or herbal 
medication (e.g., Kava, Gingko biloba, St. 
John’s wort) that may alter neuronal-
vascular coupling, with some exceptions 
below: 
 
May use anti-depressant medications. 
May use certain medications intermittently, 
provided the participant can be scanned in 
the same medication state across multiple 
study days for data consistency 
 
 
 
 
History 
 
Comprehensive Urine Drug 
Screen 
6.  
Free of current neurological illnesses 
including, but not limited to, seizure 
disorders, frequent migraines or on 
prophylaxis, multiple sclerosis, movement 
disorders (except essential tremor, so long as 
it would not interfere with study tasks such 
as button pressing), cerebrovascular 
accident, history of significant head trauma, 
or CNS tumor.   
 
 
 
 
H & P 
 
Comprehensive Urine Drug 
Screen 
 
History of head trauma (loss of 
consciousness > 30 minutes OR  
sequelae lasting >2 days)  

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 139 of 221 
 
 
7.  
Free of current major psychotic disorders, 
mania, substance-induced psychiatric 
disorders, or any current suicidal ideations or 
history of suicide attempts. Free of moderate 
to severe current symptoms of mood or 
anxiety disorders. 
 
(Mild mood or anxiety disorder symptoms 
are not exclusionary, whether medicated or 
unmedicated.) 
 
 
 
 
 
Computerized SCID,  
Clinician interview 
8.  
NOT cognitively impaired or learning 
disabled. 
 
 
 
H&P Screening (history of 
placement in special education 
classes as a consequence of 
serious learning problems, and not 
solely as a consequence of 
behavioral problems) 
 
9.  
Free of significant cardiovascular conditions 
that would make use of nicotine patch 
unsafe. 
 
 
 
 
H&P, 12-lead EKG 
10.  
Free of any other major medical condition 
that in the view of the investigators would 
compromise the safety of an individual 
during participation, or the quality of the 
data obtainable, including but not limited to 
lab values outside the following parameters:  
 
Hemoglobin < 10 g/dl  
White Blood Cell Count < 2400/µl  
Liver Function Tests > 3X upper limit of 
normal 
Serum glucose > 200 mg/dl  
Urine protein > 2+ 
Serum creatinine > 2 mg/dl 
Must be free of Diabetes Mellitus 
 
 
 
 
H&P, CBC, urinalysis, NIDA 
chemistry panel (liver function 
tests, electrolytes, kidney 
function).  
 
The MAI will retain discretion to 
exclude based on less extreme lab 
results, or other major medical 
condition not otherwise specified 
11.  
Is NOT pregnant, planning to become 
pregnant, or breastfeeding (females). 
 
 
 
 
 
Urine and/or serum pregnancy 
tests,  
Clinical interview.   
 
Females are instructed in the 
consent to use effective forms of 
birth control during the study 
period.   

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 140 of 221 
 
 
 
12.  
Has NOT participated in any brain 
stimulation session less than two weeks ago, 
or undergone brain stimulation exposure for 
treatment purposes in the last 6 months, 
including tDCS or transcranial magnetic 
stimulation (TMS). 
 
 
 
H&P, Clinical interview 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 141 of 221 
 
 
  
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 142 of 221 
 
 
Appendix 2: Participant Fact Sheets 
 
STUDY  474 Treatment Seekers FACT SHEET 
 
474: “Identifying neurobiological mechanisms that underlie acute nicotine withdrawal 
syndrome and drive early relapse in smokers” 
 
All visits will take place at the NIH/NIDA research facility on the Johns Hopkins Bayview Medical 
Campus.  
 
Participation in this study will follow 3 phases:  
 
PHASE 1:  Pre-treatment study visits: involves 3 visits over a period of about 3-5 weeks. Visit 1 will be 
an orientation visit (about 6 hours) and visits 2 and 3 will use MRI to measure brain activity (about 9 hours per 
visit). During PHASE 1 you will: 
 
• 
NOT be able to smoke for 36 hours before the two imaging visits.   
• 
Wear a nicotine skin patch or a placebo (fake) patch during your 36 hour smoking abstinence 
period and study visits. 
• 
Have your blood drawn to test for levels of stress-related hormones. 
• 
Complete multiple MRI scanning sessions that last about 1.5 to 2 hours each.  
• 
Undergo EEG (brain waves) recording. 
• 
Answer questionnaires about how you think and feel. 
• 
Complete various tasks and procedures inside and outside of the MRI scanner. 
 
Compensation: You will receive up to $911 compensation for participation in PHASE 1. 
 
PHASE 2:  Treatment visits: involves 13 visits over a period of about 12-15 weeks. Each visit will last 
about 1 hour. During PHASE 2 you will: 
 
• 
Set a quit date and develop a treatment plan with a study therapist. 
• 
Take Chantix® (varenicline) every day for a period of 12 weeks. See page 2 for more 
information on Chantix®. 
• 
Meet for weekly and biweekly counseling sessions with a therapist.  
• 
Answer questionnaires about how you think and feel. 
 
Compensation: You will receive free treatment, including medication (Chantix®) and weekly 
counseling sessions and $15 travel reimbursement for each visit to the NIH/NIDA Research facility 
 
PHASE 3:  Post-treatment follow-up visits: Approximately 1, 6 and 12 months after your last 
treatment visit you will be contacted by study staff who will schedule a follow-up visit with you. Each of the 
three follow-up visits will last approximately 1.5 hours. During PHASE 3 you will:  
Complete an MRI scanning session that will last about 20min each visit.  
Meet with a study staff member on each visit who will ask you questions about your smoking behavior 
and how you think and feel. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 143 of 221 
 
 
 
Compensation: You will receive up to $165 compensation for participation in PHASE 3. 
 
**Each phase also includes 15-30 min medical assessments with a study nurse** 
**Weekend and weeknight appointments scans and counseling available where necessary** 
 
Varenicline (Chantix®) 
 
• Varenicline (Chantix®) is approved by the FDA to help people quit smoking. 
• Varenicline is currently the most effective smoking medication available.  
• Like all medications, taking varenicline may result in side effects. The most common side effects 
include: nausea (16-34% of the people who took varenicline in clinical trials experienced this 
side effect), difficulty sleeping (18%), abnormal dreams (13%), headaches (15%), feeling tired 
or sleepy (7%) and change in appetite (3%). In rare cases heart problems have been reported.  
Low blood sugar (hypoglycemia) may also be a possibility, especially for diabetics. 
 
Other side effects that have been reported include: 
 
-vomiting 
-back and muscle pain 
-mouth sores 
-hot flush 
-stomach pain 
-disturbances in attention 
-nightmares 
-blood pressure changes 
-gas 
-dizziness 
-ringing in the ears 
-increased heart rate 
-indigestion 
-fainting 
-increased urination 
-flu-like symptoms 
-constipation 
-restlessness 
-blurred vision 
-drug allergy 
-reflux 
-anxiety 
-sweating 
-increases in weight 
-dry mouth 
-depression 
-mouth sores 
-hot flush 
-diarrhea 
-back and muscle pain 
-nightmares 
-blood pressure changes 
-irritability 
-violent/aggressive thoughts 
or actions 
-low blood sugar 
-heart problems 
-agitation 
-strange behavior 
-suicidal thoughts 
 
 
• The maker of varenicline and the FDA have issued a ‘black box’ warning indicating that 
people taking this medication should be observed for rare but serious changes in behavior 
such as agitation, depressed mood, and suicidal/violent ideation or suicidal/violent 
behavior. 
• A recent study of 80 660 men and women using smoking cessation products in the United 
Kingdom found that compared with other  smoking cessation products (e.g., nicotine 
replacement skin patches) varenicline was not associated with an increased risk of self-
harm, suicidal thoughts or suicidal behavior. 
 
 
STUDY  474 Non-treatment Seekers FACT SHEET 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 144 of 221 
 
 
 
474: “Identifying neurobiological mechanisms that underlie acute nicotine withdrawal 
syndrome  
and drive early relapse in smokers 
 
All visits will take place at the NIH/NIDA research facility on the Johns Hopkins Bayview Medical 
Campus.  
 
Participation in this study will involve 3 visits over a period of about 3-5 weeks. Visit 1 will be an orientation visit 
(about 6 hours) and visits 2 and 3 will use MRI to measure brain activity (about 9 hours per visit). During the 
study you will: 
 
• 
NOT be able to smoke for 36 hours before the two imaging visits.   
• 
Wear a nicotine skin patch or a placebo (fake) patch during your 36 hour smoking abstinence 
period and study visits. 
• 
Have your blood drawn to test for levels of stress-related hormones. 
• 
Complete multiple MRI scanning sessions that last about 1.5 to 2 hours each.  
• 
Undergo EEG (brain waves) recording. 
• 
Answer questionnaires about how you think and feel. 
• 
Complete various tasks and procedures inside and outside of the MRI scanner. 
 
• 
Compensation: You may receive up to about $911 compensation for participation in this 
study. 
 
**Each phase also includes 15-30 min medical assessments with a study nurse** 
**Weekend and weeknight appointments scans and counseling available where necessary** 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 145 of 221 
 
 
STUDY  474 tDCS FACT SHEET for PARTICIPANTS WHO SMOKE CIGARETTES 
 
474: “Transcranial Direct Current Stimulation (tDCS) to reduce effects of the Nicotine 
Withdrawal Syndrome”  
 
All visits will take place at the NIH/NIDA research facility on the Johns Hopkins Bayview Medical Campus.  
 
Brain research with functional magnetic resonance imaging (fMRI) has shown that nicotine can change some 
patterns of brain activity, and that smokers have different patterns of brain activity when compared to non-
smokers. fMRI can be used to see changes in brain patterns when a person performs various cognitive thinking 
tasks – such as a memory game – or even when lying still inside the MRI scanner. 
 
tDCS is a new technology that may help improve brain activity in both smokers and non-smokers. tDCS works 
by passing a small current through electrodes placed on your head. When tDCS is turned on, you may feel a 
tingling or “pins and needles” sensation where the electrodes are placed. This small stimulation appears to make 
some parts of the brain work a little more or less effectively. This effect goes away in less than 1 hour after the 
stimulation.  
 
This study will use fMRI to measure the effect of tDCS on brain function in both smokers and non-smokers. The 
study will also measure the effect of tDCS in smokers experiencing withdrawal. This study may help us 
understand the potential for tDCS as a future treatment for smoking. 
Participation in this study will involve 3 visits. Visit 1 will be an orientation visit (about 4.5-5.5 hours) and Visits 
2 and 3 will use MRI and tDCS to measure and stimulate brain activity (about 8 hours per visit). During the study 
you will: 
 
• 
NOT be able to smoke for 12 hours before the two imaging visits.   
• 
Wear a nicotine skin patch or a placebo (fake) patch during the day of your study visits. 
• 
Complete multiple tDCS sessions that last about 25 minutes each. There will be 3 stimulation 
sessions per day for the two imaging visits, 2 of these will be active (real) and 1 will be sham 
(fake). The tDCS brain stimulation will be given inside the MRI scanner. 
• 
tDCS requires contact with the skin on your scalp, and you may be asked to move hair out of 
the way to allow placement of tDCS electrodes. 
• 
Complete multiple MRI scanning sessions that last about 1 hour each. There will be 3 MRI 
scanning sessions per day for the two imaging visits.  
• 
There may be an additional, shorter, MRI scan on orientation day. If so, orientation will be 
~30-45 minutes longer. This scan would not include tDCS. 
• 
Answer questionnaires about how you think and feel. 
• 
Complete various tasks and procedures inside and outside of the MRI scanner. 
 
Compensation: You may receive up to about $654.50 compensation for participation in this 
study. 
**Each visit to NIDA also includes a medical assessment with a study nurse** 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 146 of 221 
 
 
STUDY  474 tDCS FACT SHEET for NONSMOKING PARTICIPANTS 
 
474: “Transcranial Direct Current Stimulation (tDCS) to reduce effects of the Nicotine 
Withdrawal Syndrome”  
 
 
All visits will take place at the NIH/NIDA research facility on the Johns Hopkins Bayview Medical Campus. 
  
Brain research with functional magnetic resonance imaging (fMRI) has shown that nicotine can change some 
patterns of brain activity, and that smokers have different patterns of brain activity when compared to non-
smokers. fMRI can be used to see changes in brain patterns when a person performs various cognitive thinking 
tasks – such as a memory game – or even when lying still inside the MRI scanner. 
 
tDCS is a new technology that may help improve brain activity in both smokers and non-smokers. tDCS works 
by passing a small current through electrodes placed on your head. When tDCS is turned on, you may feel a 
tingling or “pins and needles” sensation where the electrodes are placed. This small stimulation appears to make 
some parts of the brain work a little more or less effectively. This effect goes away in less than 1 hour after the 
stimulation.  
 
This study will use fMRI to measure the effect of tDCS on brain function in both smokers and non-smokers. The 
study will also measure the effect of tDCS in smokers experiencing withdrawal. This study may help us 
understand the potential for tDCS as a future treatment for smoking. 
 
Participation in this study will involve 2 visits. Visit 1 will be an orientation visit (about 4-5 hours) and Visit 2 will 
use MRI and tDCS to measure and stimulate brain activity (about 8 hours). During the study you will: 
 
• 
Complete multiple tDCS sessions that last about 25 minutes each. There will be 3 stimulation 
sessions on the imaging visit day, 2 of these will be active (real) and 1 will be sham (fake). The 
tDCS brain stimulation will be given inside the MRI scanner. 
• 
tDCS requires contact with the skin on your scalp, and you may be asked to move hair out of 
the way to allow placement of tDCS electrodes. 
• 
Complete multiple MRI scanning sessions that last about 1 hour each. There will be 3 MRI 
scanning sessions on the imaging visit day.  
• 
There may be an additional, shorter, MRI scan on orientation day. If so, orientation will be 
~30-45 minutes longer. This scan would not include tDCS. 
• 
Answer questionnaires about how you think and feel. 
• 
Complete various tasks and procedures inside and outside of the MRI scanner. 
 
• 
Compensation: You may receive up to about $379.50 compensation for participation in this study. 
 
**Each visit to NIDA also includes a medical assessment with a study nurse** 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 147 of 221 
 
 
STUDY  474 tDCS BEHAVIORAL PILOT FACT SHEET  
 
474: “Transcranial Direct Current Stimulation (tDCS) to reduce effects of the Nicotine 
Withdrawal Syndrome”  
 
 
All visits will take place at the NIH/NIDA research facility on the Johns Hopkins Bayview Medical Campus.  
 
tDCS is a new technology that may help improve brain activity. tDCS works by passing a small current through 
electrodes placed on your head. This small stimulation appears to make some parts of the brain work a little 
more or less effectively. This effect goes away in less than 1 hour after the stimulation. This study is a pilot study 
(a smaller study) to determine the effect of tDCS on behavior in healthy people. This study may help us 
understand the potential for tDCS as a future treatment for smoking. When tDCS is turned on, you may feel a 
tingling or “pins and needles” sensation where the electrodes are placed.  
 
Participation in this study will involve an Orientation (about 3.5 hours) and a Study Phase, where tDCS is used 
to stimulate brain activity (about 5-6 hours). The Orientation and Study Phase can be completed on the same 
or separate days. During the study you will: 
 
• 
Complete multiple tDCS sessions that last about 25 minutes each. There will be 3 stimulation 
sessions during the Study Phase. 2 of these will be active (real) and 1 will be sham (fake). The 
tDCS brain stimulation will be given inside a mock (fake) scanner. 
• 
tDCS requires contact with the skin on your scalp, and you may be asked to move hair out of 
the way to allow placement of tDCS electrodes. 
• 
Complete multiple mock scanner sessions that last about 1 hour each. There will be 3 mock 
scanner sessions during the Study Phase.  
• 
Answer questionnaires about how you normally think and feel, and how tDCS made you feel. 
• 
Complete various tasks and procedures inside and outside of the mock scanner. 
 
• 
Compensation: You may receive up to approximately $250.00 compensation for participation 
in this study if all procedures are completed on the same day. 
 
**Each visit to NIDA also includes a medical assessment with a study nurse** 
 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
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Appendix 3: Characterization Measures  
 
1) Fagerström Test for Nicotine Dependence: (Heatherton, Kozlowski, Frecker, & Fagerström, 1991) is 
a six item test that measures the severity of nicotine addiction on a 0-10 scale.  Completion time: ~>5min. 
 
Directions: Please circle the answer that best describes your typically smoking behaviors. 
 
How soon after you wake up do you smoke your first cigarette? 
After 60 minutes   (0) 
31-60 minutes       (1) 
6-30 minutes         (2) 
Within 5 minutes  (3) 
 
Do you find it difficult to refrain from smoking in places where it is forbidden? 
No                         (0) 
Yes                        (1) 
 
Which cigarette would you hate most to give up? 
The first in the morning    (1) 
Any other                          (0) 
 
How many cigarettes per day do you smoke? 
10 or less               (0) 
11-20                     (1) 
21-30                     (2) 
31 or more             (3) 
 
Do you smoke more frequently during the first hours after awakening than during the rest of the day? 
No                         (0) 
Yes                        (1) 
 
Do you smoke even if you are so ill that you are in bed most of the day? 
No                         (0) 
Yes                        (1) 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
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2) The Cigarette Dependence Scale (Etter, Le Houezec, & Perneger, 2003)is a brief 12-item self report 
that assesses the main components of DSM-IV and ICD-10 definitions of dependence, which include compulsion, 
withdrawal symptoms, loss of control, time allocation, neglect of other activities, and persistence despite harm. 
Completion time: ~5min.    
 
Directions: Please answer each of the following questions 
 
Please rate your addiction to cigarettes on a scale of 0-100 (0= I am NOT addicted to cigarettes at all, 100 
= I am extremely addicted to cigarettes) _______________. 
 
On average, how many cigarettes do you smoke per day? _______________ 
 
2b) Since your last visit how many cigarettes per day have you smoked? ____________. 
 
 
Usually, how soon after waking up do you smoke you first cigarette?   ___________minutes. 
 
For you quitting smoking for good would be: 
Impossible 
Very difficult 
Fairly difficult 
Fairly easy  
Very easy 
 
Please indicate whether you agree with each of the following statements: 
 
After a few hours without smoking, I feel an irresistible urge to smoke 
Totally agree 
Somewhat agree 
Neither agree nor disagree 
Somewhat disagree 
Totally disagree 
 
The idea of not having any cigarettes causes me stress (response options same as item 5) 
Before going out, I always make sure that I have cigarettes with me. 
I am a prisoner of cigarettes 
I smoke too much 
Sometimes I drop everything to go out and buy cigarettes  
I smoke all the time 
I smoke despite the risks to my health 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 150 of 221 
 
 
3) A general smoking history questionnaire will also be administered (Completion time: ~5 min): 
 
Directions: Please answer each of the questions regarding your smoking behavior. 
   
Number of years smoking_____________________ 
age when first cigarette smoked________________ 
age when started daily smoking________________ 
average number of cigarettes/day______________ 
number of quit attempts______________________  
strategies used during quit attempts (nicotine replacement, buproprion, counseling, cold turkey), 
 longest period of time not smoking since smoking everyday,  
Please rate your desire to quit smoking on a 0-100 scale (0= no desire to quit, 1= extreme desire to quit) 
______________________. 
Please rate your confidence in your ability to quit (0= quitting would be impossible for me, 100= It would be easy 
for me to quit)__________________________.  
Please rate your level of addiction to cigarettes (0=not addicted at all, 100= Extremely addicted) 
 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 151 of 221 
 
 
4) The Revised Social Anhedonia Scale (Eckblad, Chapman, Chapman, & Mishlove, 1982) is a 40-item 
true-false self-report questionnaire intended to measure decreased pleasure derived from interpersonal sources.  
This scale demonstrates good psychometric properties and has been extensively used in schizophrenia research 
(Edell, 1995). 
Directions: Please answer each item True or False.  Please do not skip any items.  It is important that 
you answer every item, even if you are not quite certain which is the best answer.   
 
1. 
Having close friends is not as important as many people say.                  T     F     (for each item) 
2. 
I attach very little importance to having close friends. 
3. 
I prefer watching television to going out with other people. 
4. 
A car ride is much more enjoyable if someone is with me. 
5. 
I like to make long distance phone calls to friends and relatives. 
6. 
Playing with children is a real chore. 
7. 
I have always enjoyed looking at photographs of friends. 
8. 
Although there are things that I enjoy doing by myself, I usually seem to have more fun when I do things 
with other people. 
9. 
I sometimes become deeply attached to people I spend a lot of time with. 
10. 
People sometimes think that I am shy when I really just want to be left alone. 
11. 
When things are going really good for my close friends, it makes me feel good too. 
12. 
When someone close to me is depressed, it brings me down also. 
13. 
My emotional responses seem very different from those of other people. 
14. 
When I am alone, I often resent people telephoning me or knocking on my door. 
15. 
Just being with friends can make me feel really good. 
16. 
When things are bothering me, I like to talk to other people about it. 
17. 
I prefer hobbies and leisure activities that do not involve other people. 
18. 
It's fun to sing with other people. 
19. 
Knowing that I have friends who care about me gives me a sense of security. 
20. 
When I move to a new city, I feel a strong need to make new friends. 
21. 
People are usually better off if they stay aloof from emotional involvements with most others. 
22. 
Although I know I should have affection for certain people, I don't really feel it. 
23. 
People often expect me to spend more time talking with them than I would like. 
24. 
I feel pleased and gratified as I learn more and more about the emotional life of my friends. 
25. 
When others try to tell me about their problems and hang-ups, I usually listen with interest and attention. 
26. 
I never had really close friends in high school. 
27. 
I am usually content to just sit alone, thinking and daydreaming. 
28. 
I'm much too independent to really get involved with other people. 
29. 
There are few things more tiring than to have a long, personal discussion with someone. 
30. 
It made me sad to see all my high school friends go their separate ways when high school was over. 
31. 
I have often found it hard to resist talking to a good friend, even when I have other things to do. 
32. 
Making new friends isn't worth the energy it takes. 
33. 
There are things that are more important to me than privacy. 
34. 
People who try to get to know me better usually give up after awhile. 
35. 
I could be happy living all alone in a cabin in the woods or mountains. 
36. 
If given the choice, I would much rather be with others than be alone. 
37. 
I find that people too often assume that their daily activities and opinions will be interesting 
to me. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
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38. 
I don't really feel very close to my friends. 
39. 
My relationships with other people never get very intense. 
40. 
In many ways, I prefer the company of pets to the company of people. 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 153 of 221 
 
 
 
 
5) The Physical Anhedonia Scale (Chapman & Chapman, 1978) is a 61-item true-false self-
report that taps a range of presumably pleasurable experiences involving eating, touching, feeling, 
sex, movement, smell, and sound. This scale demonstrates good psychometric properties and has 
been extensively used in schizophrenia research (Edell, 1995). 
Directions: Please answer each item True or False.  Please do not skip any items.  It is 
important that you answer every item, even if you are not quite certain which is the best answer.   
 
1. 
I have usually found lovemaking to be intensely pleasurable.          T     F (for each item) 
2. 
When eating a favorite food, I have often tried to eat slowly to make it last longer. 
3. 
I have often enjoyed the feel of silk, velvet, or fur. 
4. 
I have sometimes enjoyed feeling the strength in my muscles. 
5. 
Dancing, or the idea of it, has always seemed dull to me. 
6. 
I have always found organ music dull and unexciting. 
7. 
The taste of food has always been important to me. 
8. 
I have had very little fun from physical activities like walking, swimming, or sports. 
9. 
I have seldom enjoyed any kind of sexual experience. 
10. 
On hearing a good song, I have seldom wanted to sing along with it. 
11. 
I have always hated the feeling of exhaustion that comes from vigorous activity. 
12. 
The color that things are painted has seldom mattered to me. 
13. 
The sound of rustling leaves has never much pleased me. 
14. 
Sunbathing isn't really more fun than lying down indoors. 
15. 
There just are not many things that I have ever really enjoyed doing. 
16. 
I don't know why some people are so interested in music. 
17. 
Flowers aren't as beautiful as many people claim. 
18. 
I have always loved having my back massaged. 
19. 
I never wanted to go on any of the rides at an amusement park. 
20. 
Trying new foods is something I have always enjoyed. 
21. 
The warmth of an open fireplace hasn't especially soothed and calmed me. 
22. 
Poets always exaggerate the beauty and joys of nature. 
23. 
When I have seen a statue, I have had the urge to feel it. 
24. 
I have always had a number of favorite foods. 
25. 
I don't understand why people enjoy looking at the stars at night. 
26. 
I have had very little desire to try new kinds of foods. 
27. 
I never have the desire to take off my shoes and walk through a puddle barefoot. 
28. 
I've never cared much about the texture of food. 
29. 
When I have walked by a bakery, the smell of fresh bread has often made me hungry. 
30. 
I have often enjoyed receiving a strong, warm handshake. 
31. 
I have often felt uncomfortable when my friends touch me. 
32. 
I have never found a thunderstorm exhilarating. 
33. 
Standing on a high place and looking out over the view is very exciting. 
34. 
I have often found walks to be relaxing and enjoyable. 
35. 
The sound of the rain falling on the roof has made me feel snug and secure. 
36. 
I like playing with and petting soft little kittens or puppies. 
37. 
The sound of organ music has often thrilled me. 
38. 
Beautiful scenery has been a great delight to me. 
39. 
The first winter snowfall has often looked pretty to me. 
40. 
Sex is okay, but not as much fun as most people claim it is. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 154 of 221 
 
 
 
 
41.       I have sometimes danced by myself just to feel my body move with the music. 
42. 
I have seldom cared to sing in the shower. 
43. 
One food tastes as good as another to me. 
44. 
On seeing a soft, thick carpet, I have sometimes had the impulse to take off my shoes and 
walk barefoot on it. 
45. 
After a busy day, a slow walk has often felt relaxing. 
46. 
The bright lights of a city are exciting to look at. 
47. 
The beauty of sunsets is greatly overrated. 
48. 
It has always made me feel good when someone I care about reaches out to touch me. 
49.       I have usually found soft music boring rather than relaxing. 
50. 
I have usually finished my bath or shower as quickly as possible just to get it over with. 
51. 
The smell of dinner cooking has hardly ever aroused my appetite. 
52. 
When I pass by flowers, I have often stopped to smell them. 
53. 
Sex is the most intensely enjoyable thing in life. 
54.       I think that flying a kite is silly. 
55. 
I've never cared to sunbathe; it just makes me hot. 
56. 
The sounds of a parade have never excited me. 
57. 
It has often felt good to massage my muscles when they are tired or sore. 
58. 
When I'm feeling a little sad, singing has often made me feel happier. 
59. 
A good soap lather when I'm bathing has sometimes soothed and refreshed me. 
60. 
A brisk walk has sometimes made me feel good all over. 
61. 
I have been fascinated with the dancing of flames in a fireplace. 
 
 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 155 of 221 
 
 
 
 
 
6) Barratt Impulsiveness Scale (BIS-11, Patton et al., 1995) was designed to measure three aspects 
of behavioral and cognitive impulsiveness: attentional, motor and nonplanning. The scale has 30 
self-descriptive items to on a 4 point likert scale ranging from 1- Rarely/Never to 4 Almost 
Always/Always. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 156 of 221 
 
 
 
 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 157 of 221 
 
 
 
 
7) Rehearsal Scale of the Emotion Control Questionnaire (RS-ECQ) Roger & Najarian, 1989). 
The RS-ECQ was designed assess the trait tendency to ruminate or rehearse  
 
Instructions: Please indicate how you feel about each item by circling either ‘True’ or 
‘False’. If you feel that an item is neither entirely true nor false, please choose the 
alternative that is most like you. If you haven’t been in the situation described, please say 
how you feel you would behave in that situation. 
 
1. I remember things that upset me or make me angry for a long time afterwards. 
2. I generally don’t bear a grudge-when something is over, it’s over, and I don’t 
think about it again. 
3. I get ‘worked up’ just thinking about things that have upset me in the past. 
4. I often find myself thinking over and over about things that have made me angry. 
5. I can usually settle things quickly and be friendly again after an argument. 
6. If I see or hear about an accident, I find myself thinking about something similar 
happening to me or to people close to me. 
7. I think about ways of getting back at people who have made me angry long after 
the event has happened. 
8. I never forget people making me angry or upset, even about small things. 
9. I find it hard to get thoughts about things that have upset me out of my mind. 
10. I often daydream about situations where I’m getting my own back at people. 
11. If I see something that frightens or upsets me, the image of it stays in my mind for 
a long time afterwards. 
12. Thinking about upsetting things just seems to keep them going, so I try to put 
them out of my mind. 
13. If I lose out on something, I get over it quickly. 
14. If I have to confront someone, I try not to think too much about it beforehand. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
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tDCS Arm Questionnaires 
 
The Brief Externalizing Inventory (Hall et al., 2007), adapted from the full Externalizing inventory 
(Krueger et al., 2007), is a subset of 159 self-report items used to assess a range of behavioral and 
personality characteristics that have been attributed to a broad psychological construct termed 
externalizing.  Completion time: ~30min.     
 
 
Directions: This questionnaire contains statements that different people might use to 
describe themselves.  Most of these statements are followed by four choices T, t, f, F.  The meaning 
of these four different choices is given below: T = True    t = mostly true,   f = mostly false,    F = 
false. 
For each statement, circle the choice that describes you best. 
 
1. I have broken someone’s things to prevent them from being used.           T   t   f   F     (for each item)   
2. I've smoked marijuana before going to work or school. 
3. I have had problems at work because I was irresponsible. 
4. If I could control my impulses, my life would be much better. 
5. I enjoy pushing people around sometimes. 
6. I have lied to avoid paying back loans. 
7. I have done things that put others in danger. 
8. My drug use led to problems at work or school. 
9. I have damaged someone's things to get something I wanted. 
10. I tried an illegal drug at a party. 
11. I've told lies about someone just to see how it would affect them. 
12. I've never used marijuana in my life. 
13. I have stolen something out of a vehicle. 
14. I get in trouble for not considering the consequences of my actions. 
15. I've smoked marijuana at parties. 
16. I have been unfairly blamed when I was just taking advantage of others' mistakes. 
17. I have run up big debts that I had trouble paying. 
18. I don't see any point in worrying if what I do hurts someone else. 
19. I’ve stood friends up. 
20. I have brought a weapon into a fight. 
21. I have borrowed money with no thought of paying it back. 
22. I have rolled a marijuana joint. 
23. I have missed work without bothering to call in. 
24. I seek out thrills almost everywhere I go. 
25. I do lots of things just to get a thrill. 
26. I have not lived up to my end of a contract. 
27. I've told lies about someone who upset me. 
28. I've made big decisions without thinking them over. 
29. At some point in my life, I needed more drugs to get the same effect. 
30. I've asked someone to help bail me out of debt. 
31. I've vandalized public property just for kicks. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 159 of 221 
 
 
 
 
32. I keep appointments I make. 
33. I have thrown something at a person who angered me. 
34. Many problems in my life are caused by doing things without thinking. 
35. I sometimes insult people on purpose to get a reaction from them. 
36. People often abuse my trust. 
37. I've quit a job without giving two weeks notice. 
38. I get bored easily. 
39. I have gotten things from people by making them feel sorry for me. 
40. I have taken a drug like LSD or magic mushrooms. 
41. I don't lie very much. 
42. Others have told me they are concerned about my lack of self-control. 
43. I’ve used downers like Valium or Xanax for non-medical reasons. 
44. I have used a weapon against someone who insulted me. 
45. I often get bored quickly and lose interest. 
46. I've kept using marijuana even though it caused problems with my memory or health. 
47. At times I kept drinking alcohol even though it caused problems with family or friends. 
48. I have talked a stranger into giving me money.  
49. I have taken items from a store without paying for them. 
50. When I want something, I want it right now. 
51. I have robbed someone. 
52. I taunt people just to stir things up. 
53. I've gotten in trouble because I missed too much school. 
54. I have tried smoking marijuana. 
55. I've gone on drinking binges. 
56. I have missed a final exam. 
57. I have taken money from someone's purse or wallet without asking. 
58. I've spent more money on marijuana than I should have. 
59. I have started a fight because it was exciting. 
60. I’ve lost control of my alcohol use. 
61. I have hit someone in the face or head in anger. 
62. I have never bought drugs. 
63. I've made fun of someone to impress other people. 
64. Sometimes I threaten people. 
65. I gave up things I used to enjoy because of drugs. 
66. I have lied to get someone to sleep with me. 
67. My drinking led to problems at home. 
68. I lose control of myself and do things I probably shouldn't. 
69. I’ve held someone down to get what I wanted from them. 
70. I have written a check knowing it would not cash. 
71. I have broken into a house, school, or other building. 
72. I enjoy a good physical fight. 
73. Sometimes I use my wits to take advantage of people. 
74. At times, marijuana has been more important to me than work, friends, or school. 
75. I've used drugs when it might be hazardous, like while driving a car. 
76. People think of me as dependable. 
77. I've used marijuana when it might be hazardous, like while driving a car. 
78. I have used physical force to take something from someone. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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page 160 of 221 
 
 
 
 
79. I hate waiting to get things that I want. 
80. I have spread rumors about people who were competing with me. 
81. I’ve taken an illegal drug that gave me a rush and made me more awake. 
82. I have snuck marijuana or hash into a public event. 
83. I have used a weapon to get something I wanted. 
84. I have lost valuable goods or money because I decided things too quickly. 
85. One or more times in my life, I have beaten someone up for bothering me. 
86. I rarely lie. 
87. I’ve told lies about someone else to make myself look better. 
88. I lie sometimes without even thinking about it. 
89. I've bought items used for smoking marijuana. 
90. I've had legal problems because of my drug use. 
91. I've had legal problems because I couldn't resist my impulses. 
92. Many people consider me a rule breaker. 
93. I've gotten high using marijuana. 
94. I've ruined the friendships of people who made me angry. 
95. I've driven while drunk. 
96. I have lied to get ahead at work. 
97. I've spent big parts of my day using marijuana. 
98. I’ve never taken illegal drugs. 
99. I have a hard time waiting patiently for things I want. 
100. My impulsive decisions have caused problems with loved ones. 
101. I have gotten money from people by threatening to tell their secrets. 
102. I've never used street drugs. 
103. I think about things before I do them. 
104. How other people feel is important to me 
105. I’ve taken prescription medicine to get high. 
106. I have a habit of breaking rules. 
107. I've missed a rent or mortgage payment. 
108. I don't think about the outcomes of my decisions enough. 
109. I don’t drink. 
110. I have failed to pay my taxes on time. 
111. I get blamed for things that I don't do. 
112. I often disobey rules. 
113. I don't care much if what I do hurts others. 
114. I've thought about doing physical harm to someone who hurt me. 
115. I have damaged someone’s things because it was exciting. 
116. I have lied to the police. 
117. I have quit a job without having another source of support lined up. 
118. I often get in trouble for breaking rules. 
119. I gave up things I used to enjoy because of my drinking. 
120. My marijuana use has led to problems at home, work, or school. 
121. I have failed to show up to court when I was supposed to. 
122. I have lied on a job application. 
123. I have damaged someone’s property because I was angry with them. 
124. My drinking led to problems at work or school. 
125. I've broken something belonging to someone else to get back at them. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 161 of 221 
 
 
 
 
126. I've hurt someone's feelings on purpose to get back at them. 
127. I've failed to make payments on a loan. 
128. I don't hesitate to complicate the lives of people who upset me. 
129. I vandalized someone's house or things because they were rude to me. 
130. My drug use has caused problems with my family. 
131. I plan before I act. 
132. I've made a fool of someone because it made me feel good. 
133. I have used more drugs for longer than I meant to. 
134. I have been in trouble with the law for something I did on impulse. 
135. I have smacked someone who upset me. 
136. People use me. 
137. I have failed to pay a traffic fine. 
138. My lack of self-control gets me in trouble. 
139. I've never had any desire to try an illegal drug. 
140. I have been called a bully. 
141. I get unfairly blamed for things. 
142. I have destroyed property just for kicks. 
143. I have conned people to get money from them. 
144. I have broken into someone's home and taken things. 
145. I am sensitive to the feelings of others. 
146. I've broken the law to get money for drugs. 
147. I've skipped work or meetings to satisfy sudden urges. 
148. I have been caught shoplifting. 
149. I'm not a drinker. 
150. I have left a restaurant or gas station without paying my bill. 
151. I have bought marijuana. 
152. I like risky activities. 
153. I've gotten into trouble after blindly going after what I wanted. 
154. I have stolen something worth more than $10. 
155. I talk badly about people who cause me trouble. 
156. When I want something, nothing else seems important. 
157. I've been fired from more than one job. 
158. After trying to cut down on drinking alcohol, I've felt sad or irritable. 
159. I've hit someone because they made fun of me. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 162 of 221 
 
 
 
 
The Temperament and Character Inventory (Cloninger et al., 1994) is a widely used test that assesses dimensions 
of personality (e.g., harm avoidance, novelty seeking, reward dependence, and persistence) that are considered to 
be related to monoaminergic function.  Completion time: ~25min.   
 
Directions: In this booklet you will find statements people might use to describe their attitudes, opinions, interests, 
and other personal feelings. 
 
Each statement can be answered TRUE or FALSE.  Read the statement and describe which choice best describes 
you.  Try to describe the way you USUALLY or generally act and feel, not just how you are feeling noe 
 
We would like you to fill out this questionnaire on your own using a pencil.  When you are finished, please return 
the questionnaire. 
 
To answer you only need to circle either “T” or “F” after each question. 
 
1) I often try new things just for fun or thrills, even if most people think it is a waste of time.      T    F (for each) 
2) I usually am confident that everything will go well, even in situation that worry most people. 
3) I am often moved deeply by a fine speech or poetry. 
4) I often feel that I am the victim of circumstances. 
5) I can usually accept other people as they are, even when they are very different from me. 
6) I believe that miracles happen 
7) I enjoy getting revenge on people who hurt me. 
8) Often when I am concentrating on something, I lose awareness of the passage of time. 
9) Often I feel that my life has little purpose or meaning. 
10) I like to help find a solution to problems so that everyone comes out ahead 
11) I could probably accomplish more that I do, but I don’t see the point in pushing myself harder than is necessary 
to get by. 
12) I often feel tense and worried in unfamiliar situations, even when others feel there is little to worry about. 
13) I often do things based on how I feel at the moment without thinking about how they were done in the past. 
14) I usually do things my own way—rather than giving in to the wishes of other people. 
15) I often feel so connected to the people around me that it is like there is no separation between us. 
16) I generally don’t like people who have different ideas from me. 
17) In most situations my natural responses are based on good habits that I have developed. 
18) I would do almost anything legal in order to become rich and famous, even if I would lose the trust of many 
old friends. 
19) I am much more reserved and controlled than most people. 
20) I often have to stop what I am doing because I start worrying about what might go wrong. 
21) I like to discuss my experiences and feelings openly with friends instead of keeping them to myself. 
22) I have less energy and get tired more quickly than most people. 
23) I am often called “absent-minded” because I get so wrapped up in what I am doing that I lose track of 
everything else. 
24) I seldom feel free to choose what I want to do. 
25) I often consider another person’s feelings as much as my own. 
26) Most of the time I would prefer to do something a little risky (like riding in an automobile over steep hills 
and sharp turns) rather than having to stay quiet and inactive for a few hours. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 163 of 221 
 
 
 
 
27) I often avoid meeting strangers because I lack confidence with people I do not know. 
28) I like to please other people as much as I can. 
29) I like old “tried and true” ways of doing things much better than trying “new and improved” ways. 
30) Usually I am not able to do things according to their priority of importance to me because of lack of time. 
31) I often do things to help protect animals and plants from extinction. 
32) I often wish that I was smarter than everyone else. 
33) It gives me pleasure to see my enemies suffer. 
34) I like to be very organized and set up rules for people whenever I can. 
35) It is difficult for me to keep the same interests for a long time because my attention often shifts to something 
else. 
36) Repeated practice has given me good habits that are stronger than most momentary impulses or persuasions. 
37) I am usually so determined that I continued to work long after other people have given up. 
38) I am fascinated by the many things in life that cannot be scientifically explained. 
39) I have many bad habits that I wish I could break. 
40) I often wait for someone else to provide a solution to my problems. 
41) I often spend money until I run out of cash or get into debt from using too much credit. 
42) I think I will have very good luck in the future. 
43) I recover more slowly than most people from minor illnesses or stress. 
44) It wouldn’t bother me to be alone all the time. 
45) Often I have unexpected flashed of insight or understanding while relaxing. 
46) I don’t care very much whether other people like me or the way I do things. 
47) I usually try to get just what I want for myself because it is not possible to satisfy everyone anyway. 
48) I have no patience with people who don’t accept my views. 
49) I don’s seem to understand most people very well. 
50) You don’t have to be dishonest to succeed in business. 
51) I sometimes feel so connected to nature that everything seems to be part of one living organism. 
52) In conversations I am much better as a listener than as a talker. 
53) I lose my temper more quickly than most people. 
54) When I have to meet a group of strangers, I am more shy than most people. 
55) I am more sentimental than most people. 
56) I seem to have a “sixth sense” that sometimes allows me to know what is going to happen. 
57) When someone hurts me in any way, I usually try to get even. 
58) My attitudes are determined largely by influences outside my control. 
59) Each day I try to take another step toward my goals. 
60) I often wish I was stronger than everyone else. 
61) I like to think about things for a long time before I make a decision. 
62) I am more hard-working than most people. 
63) I often need naps or extra rest periods because I get tired easily. 
64) I like to be of service to others. 
65) Regardless of any temporary problems that I have to overcome, I always think it will turn out well. 
66) It is hard for me to enjoy spending money on myself, even when I have saved plenty of money. 
67) I usually stay calm and secure in situations that most people would find physically dangerous. 
68) I like to keep my problems to myself. 
69) I don’t mind discussing my personal problems with people whom I have known briefly or slightly. 
70) I like to stay home better than to travel or explore new places. 
71) I do not think it is smart to help weak people who cannot help themselves. 
72) I cannot have any peace of mind if I treat other people unfairly, even if they are unfair to me. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 164 of 221 
 
 
 
 
73) People will usually tell me how they feel. 
74) I often wish I could stay young forever. 
75) I am usually more upset than most people by the loss of a close friend. 
76) Sometimes I have felt like I was part of something with no limits of boundaries in time and space. 
77) I sometimes feel a spiritual connection to other people that I cannot explain in words. 
78) I try to be considerate of other people’s feelings, even when they have been unfair to me in the past. 
79) I like it when people can do whatever they want without strict rules and regulations. 
80) I would probably stay relaxed and outgoing when meeting a group of strangers, even if I were told they are 
unfriendly. 
81) Usually I am more worried than most people that something might go wrong in the future. 
82) I usually think about all the facts in detail before I make a decision. 
83) I feel it is more important to be sympathetic and understanding of other people than to be practical and though-
minded. 
84) I often feel a strong sense of unity with all the things around me. 
85) I often wish I had special powers like Superman. 
86) Other people control me too much. 
87) I like to share what I have learned with other people. 
88) Religious experiences have helped me understand the real purpose of my life. 
89) I often learn a lot from people. 
90) Repeated practice has allowed me to become good at many things that help me to be successful. 
91) I am usually able to get other people to believe me, even when I know that what I am saying is exaggerated 
or untrue. 
92) I need much extra rest, support, or reassurance to recover from minor illnesses or stress. 
93) I know there are principles for living that no one can violate without suffering in the long run. 
94) I don’t want to be richer than everyone else. 
95) I would gladly risk my own life to make the world a better place. 
96) Even after thinking about something a long time, I have learned to trust my feelings mare than my logical 
reasons. 
97) Sometimes I have felt my life was being directed by a spiritual force greater than any human being. 
98) I usually enjoy being mean to anyone who has been mean to me. 
99) I have a reputation as someone who is very practical and does not act on emotion. 
100) It is easy for me to organize my thoughts while talking to someone. 
101) I often react so strongly to unexpected news that I say or do things that I regret. 
102) I am strongly moved by sentimental appeals (like when asked to help crippled children) 
103) I usually push myself harder than most people do because I want to do as well as I possibly can. 
104) I have so many faults that I don’t like myself very much. 
105) I have too little time to look for long-term solutions for my problems. 
106) I often cannot deal with problems because I just don’t know what to do. 
107) I often wish I could stop the passage of time. 
108) I hate to make decisions based only on my first impression. 
109) I prefer spending money rather than saving it. 
110) I can usually do a good job of stretching the truth to tell a funnier story or to play a joke on someone. 
111) Even after there are problems in a friendship, I nearly always try to keep it going anyway. 
112) If I am embarrassed or humiliated, I get over it very quickly. 
113) It is extremely difficult for me to adjust to changes in my usual way of doing things because I get so tense, 
tired, or worried. 
114) I usually demand very god practical reasons before I am willing to change my old ways of doing things. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 165 of 221 
 
 
 
 
115) I need a lot of help from other people to train me to have good habits. 
116) I think that extra-sensory perception (ESP, like telepathy or precognitions) is really possible. 
117) I would like to have warm and close friends with me most of the time. 
118) I often keep trying the same thing over and over again, even when I have not had much success in a long 
time. 
119) I nearly always stay relaxed and carefree, even when nearly everyone else is fearful. 
120) I find sad songs and movies pretty boring. 
121) Circumstances often force me to do things against my will. 
122) It is hard for me to tolerate people who are different from me. 
123) I think that most things that are called miracles are just chance. 
124) I would rather be kind than to get revenge when someone hurts me. 
125)I often become so fascinated with what I’m doing that I get lost in the moment, like I’m detached from time 
and place. 
126) I do not think I have a real sense of purpose in my like. 
127) I try to cooperate with others as much as possible. 
128) I am satisfied with my accomplishments, and have little desire to do better. 
129) I often feel tense and worried in unfamiliar situations, even when other feel there is no danger at all. 
130) I often follow my instincts, hunches, or intuition without thinking through all the details. 
131) Other people often think that I am too independent because I won’t do what they want. 
132) I often feel a strong spiritual or emotional connections with all the people around me. 
133) It is usually easy for me to like people who have different values from me. 
134) I try to do as little work as possible, even when other people expect more of me. 
135) Good habits have become “second nature” to me—they are automatic and spontaneous actions nearly all the 
time. 
136) I don’t mind the fact that other people often know more than I do about something. 
137) I usually try to imagine myself “in other people’s shoes”, so I can really understand them. 
138) Principles like fairness and honesty have little role in some aspects of my life. 
139) I am better at saving money than most people. 
140) I seldom let myself get upset or frustrated: when things don’t work out, I simply move on to other activities. 
141) Even when most people feel it is not important, I often insist on things being done in a strict and orderly 
way. 
142) I feel very confident and sure of myself in almost all social situations. 
143) My friends find it hard to know my feelings because I seldom tell them about my private thoughts. 
144) I hate to change the way that I do things, even if may people tell me there is a new and better way to do it. 
145) I think it is unwise to believe in things that cannot be explained scientifically. 
146) I like to imagine my enemies suffering. 
147) I am more energetic and tire less quickly than most people. 
148) I like to pay close attention to details in everything I do. 
149) I often stop what I am doing because I get worried, even when my friends tell me everything will go well. 
150) I often with I was more powerful than everyone else. 
151) I am usually free to choose what I will do. 
152) Often I become so involved in what I am doing that I forget where I am for a while. 
153) Members of a team rarely get their fair share. 
154) Most of the time I would prefer to do something risky (like hang-gliding or parachute jumping_, rather than 
having to stay quiet and inactive for a few hours. 
155) Because I so often spend too much money on impulse, it is hard for me to save money—even for special 
plans like a vacation. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 166 of 221 
 
 
 
 
156) I don’t go out of my way to please other people. 
157) I am not shy with strangers at all. 
158) I often give in to the wishes of friends. 
159) I spend most of my time doing things that seem necessary but not really important to me. 
160) I don’t think that religious or ethical principles about what is right and wrong should have much influence 
in business decisions. 
161) I often try to put aside my own judgments so that I can better understand what other people are experiencing. 
162) Many of my habits make it hard for me to accomplish worthwhile goals. 
163) I have made real personal sacrifices in order to make the world a better place—like trying to prevent war, 
poverty, and injustice. 
164) I never worry about terrible things that might happen in the future. 
165) I almost never get so excited that I lose control of myself. 
166) I often give up a job if it takes much longer that I though it would. 
167) I prefer to start conversations, rather than waiting for others to talk to me. 
168) Most of the time I quickly forgive anyone who does me wrong. 
169) My actions are determined largely by influences outside my control. 
170) I often have to change my decisions because I had a wrong hunch or mistaken first impression. 
171) I prefer to wait for someone else to tae the lead in getting things done. 
172) I usually respect the opinions of others. 
173) I have had experiences that made my role in life so clear to me that I felt very excited and happy. 
174) It is fun for me to buy things for myself. 
175) I believe that I have experienced extra-sensory perception in my like. 
176) I believe that my brain is not working properly. 
177) My behavior is strongly guided by certain goals that I have set for my life. 
178) It is usually foolish to promote the success of other people. 
179) I often wish I could live forever. 
180) I usually like to stay cool and detached from other people. 
181) I am more likely to cry at a sad movie than most people. 
182) I recover more quickly than most people from minor illnesses or stress. 
183) I often break rules and regulations when I think I can get away with it. 
184) I need much more practice in developing good habits before I will be able to trust myself in many tempting 
situations. 
185) I wish other people didn’t talk as much as they do. 
186) Everyone should be treated with dignity and respect, even if they seem to be unimportant or bad. 
187) I like to make quick decisions so I can get on with what has to be done. 
188) I usually have good luck in whatever I try to do. 
189) I am usually confident that I can easily do things that most people would consider dangerous (such as driving 
an automobile fast on a wet or icy road). 
190) I see no point in continuing to work on something unless there is a good chance of success. 
191) I like to explore new ways to do things. 
192) I enjoy saving money more than spending it on entertainment or thrills. 
193) Individual rights are more important than the needs of any group. 
194) I have had personal experiences in which I felt in contact with a divine and wonderful spiritual power. 
195) I have had moments of great joy in which I suddenly had a clear, deep feeling of oneness with all that exists. 
196) Good habits make it easier for me to do things that way I want. 
197) Most people seem more resourceful than I am. 
198) Other people and conditions are often to blame for my problems. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 167 of 221 
 
 
 
 
199) It gives me pleasure to help others, even if they have treated me badly. 
200) I often feel like I am a part of the spiritual force on which all like depends. 
201) Even when I am with friends, I prefer not to “open up” very much. 
202) I usually can stay “ on the go” all day without having to push myself. 
203) I nearly always think about all the facts in detail before I make a decision, even when other people demand 
a quick decision. 
204) I am not very good at talking my way out of trouble when I am caught doing something wrong. 
205) I am more of a perfectionist than most people. 
206) Whether something is right or wrong is just a matter of opinion. 
207) I think my natural responses now are usually consistent with my principles and long-term goals. 
208) I believe that all life depends on some spiritual order or power that cannot be completely explained. 
209) I think I would stay confident and relaxed when meeting strangers, even if I were told they are angry at me. 
210) People find it easy to come to me for help, sympathy, and warm understanding. 
211) I am slower than most people to get excited about new ideas and activities. 
212) I have trouble telling a lie, even when it is meant to spare someone else’s feelings. 
213) There are some people I don’t like. 
214) I don’t want to be more admired than everyone else. 
215) Often when I look at an ordinary thing, something wonderful happens—I get the feeling that I am seeing it 
fresh for the first time. 
216) Many people I know look out only for themselves, no matter who else gets hurt. 
217) I usually feel tense and worried when I have to do something new and unfamiliar. 
218) I often push myself to the point of exhaustion or try to do more than I really can. 
219) Some people think I am too stingy or tight with my money. 
220) Reports of mystical experiences are probably just wishful thinking. 
221) My will power is too weak to overcome very strong temptations, even if I know I will suffer as a 
consequence. 
222) I hate to see anyone suffer. 
223) I know what I want to do in my life. 
224) I regularly take time to consider whether what I am doing is right or wrong. 
225) Things often go wrong for me unless I am very careful. 
226) If I am feeling upset, I usually fell better around friends then when left alone. 
227) I don’t think it is possible for one person to share feelings with someone else who hasn’t had the same 
experiences. 
228) It often seems to other people like I am in another world because I am so completely unaware of things going 
on around me. 
229) I wish I were better looking than everyone else. 
230) I have lied a lot on this questionnaire. 
231) I usually stay away from social situations where I would have to meet strangers, even if I am assured that 
they will be friendly. 
232) I love the blooming of flowers in the spring as much as seeing an old friend again. 
233) I usually look at a difficult situation as a challenge or opportunity. 
234) People involved with me have to learn how to do things my way. 
235) Dishonesty only causes problems if you get caught. 
236) I usually feel much more confident and energetic than most people, even after minor illnesses or stress. 
237) I like to read everything when I am asked to sign any papers. 
238) When nothing new is happening, I usually start looking for something that is thrilling or exciting. 
239) Sometimes I get upset. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 168 of 221 
 
 
 
 
240) Occasionally I talk about people behind their backs. 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 169 of 221 
 
 
 
 
Sensation Seeking Scale V (SSS-V, Zuckerman et al., 1978) is a 40-item self-report questionnaire that assesses 
individual differences in sensation seeking.  The scale consists of four subscales (Boredom Susceptibility [BS], 
Thrill and adventure seeking [TAS], Experience seeking [ES], and Disinhibition [Dis]) composed of 10-items 
each.  Completion time: ~5min. 
 
Directions: Please rate each item with a True or False indication by circling the T or F next to each item. 
 
-BS Items: 
1) I can’t stand watching a movie that I’ve seen before.                   T    F   (for each item) 
2) I get bored seeing the same old faces. 
3) When you can predict almost everything a person will do and say he or she must be a bore. 
4) I usually don’t enjoy a movie or play where I can predict what will happen in advance 
5) Looking at someone’s home movies or travel slides bores me tremendously. 
6) I prefer friends who are excitingly unpredictable. 
7) I get restless if I have to stay around home for any length of time. 
8) The worst social sin is to be a bore. 
9) I have no patience with dull or boring persons. 
10) I like people who are sharp and witty even if they do sometimes insult people. 
 
-TAS Items 
1) I often wish I could be a mountain climber 
2) I sometimes like to do things that are a little frightening. 
3) I would like to take up the sport of water-skiing. 
4) I would like to try surfboard riding. 
5) I would like to learn to fly an airplane. 
6) I would like to go scuba diving. 
7) I would like to try parachute jumping. 
8) I like to dive off the high board. 
9) I would like to sail a long distance in a small but seaworthy sailing craft. 
10) I think I would enjoy the sensations of skiing very fast down a high mountain slope. 
 
-ES items: 
1) I like some of the earthy body smells. 
2) I like to explore a strange city or section of town myself, even if it means getting lost. 
3) I have tried marijuana or would like to. 
4) I would like to try some of the new drugs that produce hallucinations. 
5) I like to try new foods that I have never tasted before. 
6) I would like to take off on a trip with no pre-planned or definite routes or timetables. 
7) I would like to make friends in some of the “far-out” groups like artists or “hippies”. 
8) I would like to meet some persons who are homosexual (men or women). 
9) I often find beauty in the “clashing” of colors and irregular forms of modern painting. 
10) People should dress in individual ways even if the effects are sometimes strange. 
 
-DIS items: 
1) I like wild “uninhibited” parties. 
2) I enjoy the company of real “swingers”. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 170 of 221 
 
 
 
 
3) I often like to get high (drinking liquor or smoking marijuana). 
4) I like to have new and exciting experiences and sensations even if they are a little unconventional or illegal 
5) I like to meet members of the opposite sex who are physically exciting. 
6) Keeping the drinks full is the key to a good party. 
7) A person should have considerable sexual experience before marriage. 
8) I could conceive of myself seeking pleasures around the world with the “jet set”.  
9) I enjoy watching many of the “sexy” scenes in movies. 
10) I feel best after taking a couple of drinks. 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 171 of 221 
 
 
 
 
Attitudes Towards Risk Questionnaire consists of 34-self report items rated on a 5-point scale that assess 
attitudes towards physical and psychological risk. Completion time: ~5min. 
 
Directions: Indicate, using the 5-point scale, the degree to which each of the following statements describes 
you. Use the number 1 if the statement is a very good description of you (like me) and the number 5 to 
indicate it does not describe you at all (not like me). Use remaining numbers to indicate the varying degrees 
that the statement is like you or not like you. 
Like me                                                                Not like me 
1..................2...................3...................4...................5 
 
1. I like the feeling that comes with taking physical risks. 
2. I like the feeling that comes with taking psychological or social risks. 
3. While I don’t deliberately seek out situations or activities that involve physical risk, I often end up doing 
things that involve physical risk. 
4. I often seek out situations or activities that society does not approve of. 
5. While I don’t deliberately seek out situations or activities that society disapproves of, I find that I often 
end up doing things that society disapproves of. 
6. I often do things that I know my parents would disapprove of. 
7. I often do things that I know some of my friends would disapprove of. 
8. I often find that I am anxious or even scared of things that I am about to do. 
9. I often do things that would hurt my reputation. 
10. I often do things that would jeopardize my reputation. 
11. I often do things that could jeopardize my friendships. 
12. I never let fear get in the way of my doing things. 
13. I like the feeling that comes from entering a new situation. 
14. I don’t let what other people think prevent me from doing new things. 
15. I like to risk large sums of money. 
16. I would be willing to risk my life in order to receive 10 million dollars. 
17. I consider myself a risk-taker. 
18. Being afraid of doing something new often makes it more fun in the end. 
19. The greater the risk the more fun the activity. 
20. I like to do things that almost paralyze me with fear. 
21. I really don’t care what people think of what I say and do. 
22. I do not let the fact that something is illegal stop me from doing it. 
23. I do not let the fact that something is considered immoral stop me from doing it. 
 
Some people don’t actually take risks but think about them. The following questions pertain to how much 
you think about risks: 
24. I often think about doing activities that involve physical risk. 
25. I often think about doing activities that involve social risk. 
26. I often think about doing things that might jeopardize my health. 
27. I often think about doing things that I know my friends would disapprove of. 
28. I often think about doing things that I know my parents would disapprove of. 
29. I often think about doing things that would arouse a great deal of fear or anxiety in me. 
30. I often think about doing things that I know society would disapprove of. 
31. I often think about doing things that are illegal. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 172 of 221 
 
 
 
 
32. I often think about doing things that are considered immoral. 
33. I often think about doing things that would make me a lot of money. 
34. I often think about things that would make me famous or notorious. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 173 of 221 
 
 
 
 
Toronto Alexithymia Scale (TAS-20): a 20-item self report questionnaire to assess emotional 
awareness. Completion time: ~5min. 
 
Using the scale provided as a guide, indicate how much you agree or disagree with each 
of the following statements by circling the corresponding number.  Give only one answer 
for each statement. 
 
Circle1 if you STRONGLY AGREE 
Circle 2 if you MODERATELY AGREE 
Circle 3 if you NEITHER AGREE NOR DISAGREE 
Circle 4 if you MODERATELY AGREE 
Circle 5 if you STRONGLY AGREE 
 
1) 
I am often confused about what emotion I am feeling. 
2) 
It is difficult for me to find the right words for my feelings. 
3) 
I have physical sensations that even doctors don’t understand. 
4) 
I am able to describe my feelings easily. 
5) 
I prefer to analyze problems rather than just describe them. 
6) 
When I am upset, I don’t know if I am sad, frightened, or angry. 
7) 
I am often puzzled by sensations in my body. 
8) 
I prefer to just let things happen rather than to understand why they turned out 
that way. 
9) 
I have feelings that I can’t quite identify. 
10) 
Being in touch with emotions is essential. 
11) 
I find it hard to describe how I feel about people. 
12) 
People tell me to describe my feelings more. 
13) 
I don’t know what’s going on inside me. 
14) 
I often don’t know why I am angry. 
15) 
I prefer talking to people about their dily activities rather than their feelings. 
16) 
I prefer to watch “light” entertainment shows rather then psychological dramas. 
17) 
It is difficult for me to reveal my innermost feelings, even to close friends. 
18) 
I can feel close to someone, even in moments of silence. 
19) 
I find examination of my feelings useful in solving personal problems. 
20) 
Looking for hidden meanings in movies or plays distracts from their enjoyment. 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 174 of 221 
 
 
 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 175 of 221 
 
 
 
 
Beck Depression Inventory (BDI) 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 176 of 221 
 
 
 
 
Beck Anxiety Inventory 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 177 of 221 
 
 
 
 
Adult ADHD Self Report Scale (ASRS) 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 178 of 221 
 
 
 
 
Drug Use Survey 
 
 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 179 of 221 
 
 
 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 180 of 221 
 
 
 
 
Appendix 4: State Measures (*administered during treatment 
sessions; #administered during scan sessions; + administered during follow-up 
scan sessions) 
*#+1) The Tobacco Craving Questionnaire (Singleton, Anderson, & Heishman, 2003) is a brief instrument used 
to assess current feelings related to smoking and craving using 12 Likert-type items.  Each item is rated on a 7-
point scale from strongly disagree to strongly agree.  Completion time: ~10min.  
 
Directions: Indicate how strongly you agree or disagree with each of the following 
statements by placing a check mark in one of the spaces between STRONGLY DISAGREE and 
STRONGLY AGREE.  The closer you place your check mark to one end or the other indicates the 
strength of your agreement or disagreement.  If you don’t agree or disagree with a statement, 
place your check mark in the middle space.  Please complete every item.  We are interested in 
how you are thinking or feeling right now as you are filling out the questionnaire. 
 
1. I would enjoy a cigarette right now. 
STRONGLY DISAGREE  ____ : ____ : ____ : ____ : ____ : ____ : ____  STRONGLY AGREE 
 
2. If I smoked right now, I would not be able to stop. 
STRONGLY DISAGREE  ____ : ____ : ____ : ____ : ____ : ____ : ____  STRONGLY AGREE 
 
3. If I had a lit cigarette in my hand, I probably would smoke it. 
STRONGLY DISAGREE  ____ : ____ : ____ : ____ : ____ : ____ : ____  STRONGLY AGREE 
 
4. A cigarette would taste good right now. 
STRONGLY DISAGREE  ____ : ____ : ____ : ____ : ____ : ____ : ____  STRONGLY AGREE 
 
5. I would be less irritable now if I could smoke. 
STRONGLY DISAGREE  ____ : ____ : ____ : ____ : ____ : ____ : ____  STRONGLY AGREE 
 
6. It would be hard to pass up the chance to smoke. 
STRONGLY DISAGREE  ____ : ____ : ____ : ____ : ____ : ____ : ____  STRONGLY AGREE 
 
7. I could not stop myself from smoking if I had some cigarettes here. 
STRONGLY DISAGREE  ____ : ____ : ____ : ____ : ____ : ____ : ____  STRONGLY AGREE 
 
8. Smoking a cigarette would be pleasant. 
STRONGLY DISAGREE  ____ : ____ : ____ : ____ : ____ : ____ : ____  STRONGLY AGREE 
 
9. If I were smoking now I could think more clearly. 
STRONGLY DISAGREE  ____ : ____ : ____ : ____ : ____ : ____ : ____  STRONGLY AGREE 
 
10. I would not be able to control how much I smoked if I had some cigarettes here. 
STRONGLY DISAGREE  ____ : ____ : ____ : ____ : ____ : ____ : ____  STRONGLY AGREE 
 
11. I could not easily limit how much I smoked right now. 
STRONGLY DISAGREE  ____ : ____ : ____ : ____ : ____ : ____ : ____  STRONGLY AGREE 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 181 of 221 
 
 
 
 
 
12. I could control things better right now if I could smoke. 
STRONGLY DISAGREE  ____ : ____ : ____ : ____ : ____ : ____ : ____  STRONGLY AGREE 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 182 of 221 
 
 
 
 
*#+2) Tobacco Craving Scale consists of 5 self-report items pertaining to desire for a cigarette that 
are rated on a 10-point scales. Completion time: > 5 min. 
 
Directions: Please circle the number on the scale that best describes your current feelings. 
 
Please rate how strong your desire for a cigarette is right now.  
     No desire 
 
 
 
 
 
 
    
 
 
Extremely strong 
0 
1 
2 
3 
4 
5 
6 
7 
8 
9 
10 
 
 
Please rate how strong your desire for a cigarette was during the last 24 hours. 
     No desire 
 
 
 
 
 
 
 
      
 
Extremely strong 
0 
1 
2 
3 
4 
5 
6 
7 
8 
9 
10 
 
 
Please rate how often you had the urge to smoke during the past 24 hours. 
     Not at all 
 
 
 
 
 
 
 
          
 
Extremely often 
0 
1 
2 
3 
4 
5 
6 
7 
8 
9 
10 
 
 
In the past 24 hours, please rate how strong your urges have been for a cigarette when 
something in the environment has reminded you of it.  
     No urges 
 
 
 
 
 
 
 
        
 
Extremely strong 
0 
1 
2 
3 
4 
5 
6 
7 
8 
9 
10 
 
 
Please imagine yourself in the environment in which you previously used drugs and/or 
alcohol. If you were in this environment right now, what is the likelihood that you would smoke? 
     Not at all 
 
 
 
 
 
 
   
    
I’m 
sure I would use 
0 
1 
2 
3 
4 
5 
6 
7 
8 
9 
10 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 183 of 221 
 
 
 
 
*#+3) Wisconsin Smoking Withdrawal Scale (WSWS) is a 28-item scale that measures the severity 
of smoking withdrawal symptoms. 
 
Please answer the following questions based on how you have felt or what you have noticed 
over the last 24 hours. Answer based on how you have felt in general during this time.  
0 
1 
2 
3 
4 
Strongly 
disagree 
Disagree 
Feel neutral 
Agree 
Strongly agree 
 
1. Food is not particularly appealing to me 
2. I am getting restful sleep 
3. I have been tense or anxious 
4. My level of concentration is excellent 
5. I awaken from sleep frequently during the night 
6. I have felt impatient 
7. I have felt upbeat and optimistic 
8. I have found myself worrying about my problems 
9. I have had frequent urges to smoke 
10. I have felt calm lately 
11. I have been bothered by the desire to smoke a cigarette 
12. I have felt sad or depressed 
13. I have been irritable, easily angered 
14. I want to nibble on snacks or sweets 
15. I have been bothered by negative moods such as anger, frustration, and 
irritability 
16. I have been eating a lot. 
17. I am satisfied with my sleep 
18. I  have felt frustrated 
19. I have felt hopeless or discouraged 
20. I have thought about smoking a lot. 
21. I have felt hungry 
22. I feel that I am getting enough sleep. 
23. It is hard to pay attention to things 
24. I  have felt happy and content 
25. My sleep has been troubled 
26. I have trouble getting cigarettes off my mind 
27. It has been difficult to think clearly. 
28. I think about food a lot. 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 184 of 221 
 
 
 
 
*#+4) The Positive and Negative Affect Schedule (Watson, Clark, & Tellegen, 1988) is a 20-item 
scale composed of 10 items describing negative affect and 10 items describing positive affect.  
Completion time: ~5 min. 
 
Directions: This scale consists of a number of words that describe different feelings and 
emotions. Read each item and then select the appropriate answer. Indicate to what extent you feel 
this way RIGHT NOW, that is, AT THE PRESENT MOMENT. 
 
(1) = Very slightly 
or not at all 
(2) 
= 
A 
little 
(3) = Moderately 
(4) = Quite a bit 
(5) = Extremely 
 
 
Very slightly 
or not at all 
A little 
Moderately 
Quite a bit 
Extremely 
1. Interested 
1 
2 
3 
4 
5 
2. Distressed 
1 
2 
3 
4 
5 
3. Excited 
1 
2 
3 
4 
5 
4. Upset 
1 
2 
3 
4 
5 
5. Strong 
1 
2 
3 
4 
5 
6. Guilty 
1 
2 
3 
4 
5 
7. Scared 
1 
2 
3 
4 
5 
8. Hostile 
1 
2 
3 
4 
5 
9. Enthusiastic 
1 
2 
3 
4 
5 
10. Proud 
1 
2 
3 
4 
5 
11. Irritable 
1 
2 
3 
4 
5 
12. Alert 
1 
2 
3 
4 
5 
13. Ashamed 
1 
2 
3 
4 
5 
14. Inspired 
1 
2 
3 
4 
5 
15. Nervous 
1 
2 
3 
4 
5 
16. Determined 
1 
2 
3 
4 
5 
17. Attentive 
1 
2 
3 
4 
5 
18. Jittery 
1 
2 
3 
4 
5 
19. Active 
1 
2 
3 
4 
5 
20. Afraid 
1 
2 
3 
4 
5 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 185 of 221 
 
 
 
 
#5) Visual analog scales  will also be used to assess the degree to which various nicotine withdrawal 
symptoms may be experienced, including stress, anxiety, and desire to smoke (craving). 
Completion time ~5 min. 
 
     Mark:        BASELINE        POST   
 
 
Scan 1  
Scan 2 
Please read/answer the following questions. Think about how you are feeling right now.  
1. How stressed are you?  
     Not at all        
          
 
 
 
 
 
       Extremely                               
            
0 
1 
2 
3 
4 
5 
6 
7 
8 
9 
10 
 
2. How anxious are you?  
 
     Not at all        
          
 
 
 
 
 
       Extremely                               
            
0 
1 
2 
3 
4 
5 
6 
7 
8 
9 
10 
 
3. How much are you craving a cigarette?  
 
Not at all        
          
 
 
 
 
 
       Extremely                               
            
0 
1 
2 
3 
4 
5 
6 
7 
8 
9 
10 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 186 of 221 
 
 
 
 
#+6)  The State-Trait Anxiety Inventory Form Y (STAI) (Spielberger, 1983) is an instrument for 
measuring anxiety in adults. Participants will complete the state anxiety form which measures the 
temporary condition of "state anxiety" characterized by feelings of apprehension, tension, 
nervousness, and worry. Completion time: ~10min. 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 187 of 221 
 
 
 
 
*#+7) Snaith-Hamilton pleasure scale (Snaith et al., 1995) is designed to measure hedonic tone.  
Subjects indicate the extent to which they agree or disagree with a series of statements relating to 
their expected enjoyment of a range of normally pleasurable events. 
 
Directions: This questionnaire is designed to measure your ability to experience pleasure 
during your current abstinence period.  It is important to read each statement very carefully. Check 
one of the boxes [ ] to indicate how much you agree or disagree with each statement. 
 
For each item: [ ] strongly disagree  [ ] disagree  [ ] agree  [ ] strongly agree 
 
1) I would enjoy my favorite television or radio program: 
2) I would enjoy being with my family or close friends: 
3) I would find pleasure in my hobbies and pastimes: 
4) I would be able to enjoy my favorite meal: 
5) I would enjoy a warm bath or refreshing shower: 
6) I would find pleasure in the scent of flowers or the smell of a fresh sea breeze or freshly baked bread: 
7) I would enjoy seeing other people’s smiling faces: 
8) I would enjoy looking “smart” when I have made and effort with my appearance: 
9) I would enjoy reading a book, magazine, or newspaper: 
10) I would enjoy a cup of tea or coffee or my favorite drink: 
11) I would find pleasure in small things, e.g., bright sunny day, a telephone call from a friend: 
12) I would be able to enjoy a beautiful landscape or view: 
13) I would get pleasure from helping others: 
14) I would feel pleasure when I receive praise from other people: 
 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 188 of 221 
 
 
 
 
*8) Time-line followback. (TLFB). The TLFB will be used to assess smoking behavior  since last 
an individual’s last treatment visit (e.g., 7 days).  Participants will read the instructions and will be 
guided by the therapist in filling out the calendar.  
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 189 of 221 
 
 
 
 
Instructions for Filling Out the Timeline  
Cigarette Use Calendar 
To help us evaluate your cigarette use, we need to get an idea of what 
your smoking was like in the past ____ days. To do this, we would like you to fill 
out the attached calendar.  
 Filling out the calendar is not hard! 
 Try to be as accurate as possible. 
 We recognize you won’t have perfect recall. That’s OKAY. 
 
 WHAT TO FILL IN 
• The idea is to record how many cigarettes you smoked for each day on the 
calendar. 
• On days when you did not smoke cigarettes, not even one, you should write a 
“0.”  
It’s important that something is written for every day, even 
if it is a “0”. 
 
 YOUR BEST ESTIMATE  
• We realize it isn’t easy to recall things with 100% accuracy.  
• If you are not sure whether you smoked 15 or 16 cigarettes or whether you 
smoked on a Thursday or a Friday, give it your best guess! What is important 
is that 15 or 16 cigarettes is very different from 1 cigarette. The goal is to get 
a sense of how frequently you smoked, how much you smoked, and your 
patterns of smoking. 
 
 HELPFUL HINTS 
• If you have an appointment book you can use it to help you recall your use. 
• Holidays such as Thanksgiving and Christmas are marked on the calendar to 
help you recall your smoking. Also, think about how much you smoked on 
personal holidays & events such as birthdays, vacations, or parties.  
• If you have regular patterns to your smoking, you can use these to help you 
recall your use. For example, some people may only smoke during social 
situations. 
 
 COMPLETING THE CALENDAR 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 190 of 221 
 
 
 
 
• A blank calendar is attached. Write in the number of cigarettes you smoked on 
each day. 
• The time period we are talking about on the calendar is  
from ________________________ to _______________________. 
• In estimating the number of cigarettes you smoked, be as accurate as possible. 
• DOUBLE CHECK THAT ALL DAYS ARE FILLED IN BEFORE RETURNING 
THE CALENDAR.  
• Before you start look at the SAMPLE CALENDAR on the next page.  
 
 Instructions for Filling Out the Timeline Cigarette Calendar 
 
 SAMPLE CALENDAR 
 
 
2000 
SUN 
MON 
TUES 
WED 
THURS 
FRI 
SAT 
 
 
 
 
 
 
1 
20 
2 
0 
S 
3 
20 
4 Labor Day 
20 
5 
23 
6 
28 
7 
21 
8 
20 
9 
23 
E 
10 
20 
11 
20 
12 
20 
13 
28 
14 
25 
15 
0 
16 
24 
P 
17 
20 
18 
20 
19 
20 
20 
20 
21 
22 
22 
22 
23 
24 
T 
24 
21 
25 
22 
26 
26 
27 
24 
28 
23 
29 
0 
30 
22 
 
CDW Entry (for investigator entry): 
TLFB – Tobacco Cigarettes 
 
Date of Last Visit:_________ 
Enter the number of cigarettes smoked per day since the last session, starting with 
yesterday and working backwards. 
Day 1 (yesterday) 
Day 2 
Day 3 
Etc… 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 191 of 221 
 
 
 
 
*#+9) Perceived Stress Scale (PSS; Cohen, Kamarck, & Mermelstein, 1983). The PSS is a brief 
instrument used to assess degree of perceived stress during the past month using 10 Likert-type 
items. Each item is rated on a 5-point scale from never to very often. Completion time: ~5min.  
 
The questions in this scale ask you about your feelings and thoughts during the 
last month. In each case, you will be asked to indicate by circling how often you felt or 
thought a certain way. 
 
0 = Never 1 = Almost Never 2 = Sometimes 3 = Fairly Often 4 = Very Often 
 
1. In the last month, how often have you been upset 
because of something that happened unexpectedly?..................................... 0 1 2 3 4 
 
2. In the last month, how often have you felt that you were unable 
to control the important things in your life?...................................................... 0 1 2 3 
4 
 
3. In the last month, how often have you felt nervous and “stressed”? .............. 0 1 2 3 
4 
 
4. In the last month, how often have you felt confident about your ability 
to handle your personal problems?................................................................. 0 1 2 3 4 
 
5. In the last month, how often have you felt that things 
were going your way?...................................................................................... 0 1 2 3 4 
 
6. In the last month, how often have you found that you could not cope 
with all the things that you had to do? ............................................................. 0 1 2 3 4 
 
7. In the last month, how often have you been able 
to control irritations in your life?....................................................................... 0 1 2 3 
4 
 
8. In the last month, how often have you felt that you were on top of things?..... 0 1 2 
3 4 
 
9. In the last month, how often have you been angered 
because of things that were outside of your control?..................................... 0 1 2 3 4 
 
10. In the last month, how often have you felt difficulties 
were piling up so high that you could not overcome them?............................ 0 1 2 3 4 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 192 of 221 
 
 
 
 
tDCS Arm Questionnaires 
 
The Minnesota Nicotine Withdrawal scale (Hughes and Hatsukami, 1998) yields a measure of total 
withdrawal related discomfort based on ratings from 15 self-report items.  Completion time: ~5 
min. 
 
Directions: Please rate the degree to which you have experienced the following, over the past 24 
hrs. 
 
0=none, 1=slight, 2=mild, 3=moderate, 4=severe 
 
1) Angry, Irritable, frustrated                                   0 1 2 3 4 
2) Anxious, Nervous                                                0 1 2 3 4  
3) Depressed mood, Sad                                           0 1 2 3 4 
4) Desire or craving to smoke                                   0 1 2 3 4 
5) Difficulty concentrating                                        0 1 2 3 4 
6) Increased appetite                                                  0 1 2 3 4 
7) Insomnia, sleep problems, awakening at night     0 1 2 3 4 
8) Restless                                                                  0 1 2 3 4 
9) Impatient                                                               0 1 2 3 4 
10) Constipation                                                           0 1 2 3 4 
11) Dizziness                                                               0 1 2 3 4 
12) Coughing                                                               0 1 2 3 4 
13) Dreaming or Nightmares                                       0 1 2 3 4 
14) Nausea                                                                   0 1 2 3 4 
15) Sore throat                                                              0 1 2 3 4 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 193 of 221 
 
 
 
 
Visual analog scales (100mm) will also be used to assess the degree to which various nicotine 
withdrawal symptoms may be experienced: depressed, clumsy, tired, anxious, happy, drowsy, sad, 
dizzy, alert, energetic, light-headed, irritable, frustrated, nervous, sad, desire to smoke, difficulty 
concentrating, increased appetite, hunger, weight-gain, difficultly sleeping, awakening at night, 
insomnia, restless, impatient, panicked, missing cigarettes, urge to smoke, disoriented.  Completion 
time ~10 min. 
(This Visual Analog Scale is a different version from the one given as part of the Main Study Arm.) 
 
 
 
Not at all 
Extreme 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
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The Profile of Mood States (POMS) measures present mood state (disturbance) by a list of adjectives on a 
5-point scale and measures six dimensions of affect or mood, including tension-anxiety, depression-
dejection, anger-hostility, vigor-activity, fatigue-inertia, and confusion-bewilderment. The measure has 
been shown to produce reliable and valid profiles of mood state (McNair et al., ; Cella et al., 1989). 
Completion time ~10min. 
 
Directions: Indicate HOW YOU FEEL RIGHT NOW by selecting one of the numbers on the 5-poit scale. 
 
FEELING                            Not at all             A little            Moderate          Quite a bit         Extremely  
Friendly                                     1                         2                     3                          4                       5 
 
Tense  
Angry  
Worn Out  
Unhappy  
Clear-headed  
Lively 
Confused 
Sorry for things done 
Shaky 
Listless 
Peeved 
Considerate 
Sad 
Active 
On edge 
Grouchy 
Blue 
Energetic 
Panicky 
Hopeless 
Relaxed 
Unworthy  
Spiteful  
Sympathetic  
Uneasy  
Restless  
Unable to concentrate  
Fatigued  
Helpful  
Annoyed  
Discouraged  
Resentful  
Nervous 
Lonely  
Miserable  
Muddled 
Cheerful  
Bitter  
Exhausted  
Anxious  
Ready to fight  
Good-natured  
Gloomy  
Desperate  
Sluggish  
Rebellious  
Helpless  
Weary  
Bewildered  
Alert  
Deceived  
Furious  
Efficacious  
Trusting  
Full of pep  
Bad-tempered  
Worthless  
Forgetful  
Carefree  
Terrified  
Guilty  
Vigorous  
Uncertain about things  
Bushed 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 195 of 221 
 
 
 
 
The State-Trait Anxiety Inventory Form Y (STAI) is an instrument for measuring anxiety in adults. 
The STAI differentiates between the temporary condition of "state anxiety" and the more general and 
long-standing quality of "trait anxiety." The essential qualities evaluated by the STAI-anxiety scale 
are feelings of apprehension, tension, nervousness, and worry. Completion time: ~10min. 
The copy below includes the Trait Inventory (in addition to the State Inventory given in  the Main 
Study Arm) 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 196 of 221 
 
 
 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 197 of 221 
 
 
 
 
Menstrual History Questionnaire 
Adapted from the Fernald Community Cohort/Medical Monitoring Program 
2007 Questionnaire (Female).  
University of Cincinnati College of Medicine, Department of Environmental Health. 
URL: 
https://med.uc.edu/eh/research/projects/fcc/questionnaire, 
https://med.uc.edu/docs/default-
source/Environmental-Health/fcc/y2007-questionnaire_female.pdf?sfvrsn=0 
 
1. How old were you when you started having menstrual periods? 
Age: _________  
1a. If you cannot remember your exact age, were you: 
□ Younger than 10  
□ 16 or older 
□ 10-12 yrs old  
□ 13-15 yrs old  
□ Don’t Know 
 
2. At present which statement best describes your menstrual cycle? 
□ I’m still having regular periods: The date of my last period was: ___/___/_____ 
□  My periods are irregular: The date of my last period was: ___/___/_____ 
□  I’m pregnant, or my last pregnancy ended within the past 2 months, 
 or I’m breast feeding 
□ My periods have stopped on their own. (I’ve had menopause.) 
□ I’ve had menopause, but now have periods because I am taking hormones. 
□ I’ve had an operation (surgery) which stopped my periods. 
 
If your menstrual periods ceased because of surgery, what did you have 
removed? 
□ One ovary only  
□ Uterus only 
□ Both ovaries  
□ Uterus and one ovary 
□ Uterus and both ovaries 
□ Don’t know 
□ I’ve taken medication which has stopped my periods. 
 
If your periods stopped because of medication, which medication were you 
taking? Medication name: _____________________________________ 
□ I”ve had chemotherapy which has stopped my periods. 
□ I’ve had radiation therapy which has stopped my periods. 
□ Other: _____________________________________________________ 
 
3. If your menstrual periods have stopped, how old were you when your menstrual 
periods stopped? (Please provide us with the age at which your menstrual periods stopped 
regardless of why they have stopped – naturally, due to surgery, medication, chemotherapy, 
or radiation therapy. If your periods have stopped, but you now have periods because of 
taking hormones, answer with the age at which your periods first stopped.) 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 198 of 221 
 
 
 
 
 Were you:  
□ Younger than 20  
□ 45-49 yrs old 
□ 20-29 yrs old  
□ 50-54 yrs old 
□ 30-39 yrs old  
□ 55 – 59 yrs old 
□  40-44 yrs old  
□ 60 or older 
 OR □ My menstrual periods have not stopped. 
 
4. If your menstrual periods have stopped, how old were you when you first 
experienced symptoms of menopause such as hot flashes or night sweats? 
_____ Years old □ Did not experience symptoms 
□ Don’t Know 
OR □ My menstrual periods have not stopped.  
 
All women should answer the next two questions, whether they currently have 
menstrual periods or not. 
 
5. When you are (were) having regular menstrual cycles, how many days are (were) 
there between periods? _______ Days between periods 
For how many days do (did) you have your period? _______ Days 
 
6. Between the ages of 18 and 40, excluding times when you may have been on the 
pill, pregnant, or nursing, which of the following statements BEST describes your 
menstrual periods? They are (were)… 
□ Nearly always regular, that is, you could usually predict when you would 
start bleeding to within two or three days 
□ Fairly Regular 
□ Irregular 
□ Don’t Know 
 
 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 199 of 221 
 
 
 
 
Appendix 5: Nicotine Patch Instructions  
 
Read ALL the instructions before you begin.  
Your abstinence period begins at 8pm, two days before your next NIDA visit (e.g., 8pm 
Saturday night if your visit is at 8am on Monday morning). Smoke a cigarette after dinner, before 
you commence the abstinence period, if this is usual for you. You will receive a phone call from a 
study investigator between 5pm and 8pm to ensure that you remember and understand the 
abstinence procedure. You will place the patch on as soon as you wake up the following day, that 
is, when you would typically have your first cigarette. To place and change the patch, follow the 
instructions outlined below. Do not smoke any cigarettes while you are wearing the patch. Since 
the nicotine patch works by delivering small doses of nicotine into the body, smoking may cause 
harmful levels of nicotine in your body. 
 
How to apply the nicotine patch: 
1. Do not remove the patch from its sealed protective pouch until you are ready to use it. 
2. Choose a non-hairy, clean, dry area of skin. The upper arm is OK; we recommend the back 
where we will place the patch during the study. Do not put the patch on skin that is burned, 
broken out, cut, or irritated in any way. Make sure your skin is free of lotion and soap 
before applying the patch. 
3. A clear, protective liner covers the sticky backside of the patch – the side that will be put 
on your skin. The liner has a slit down the middle to help you remove it from the patch. 
With the sticky backside facing you, pull half the liner away from the patch starting at the 
middle slit, as shown in the illustration below. Hold the patch at one of the outside edges 
(touch the sticky side as little as possible), and pull off the other half of the protective liner.   
                                     
 
4. Immediately apply the sticky side of the patch to your skin. Press the patch firmly on 
your skin with the palm of your hand for at least 10 seconds. Make sure it sticks well 
to your skin, especially around the edges. Mild itching, burning or tingling is normal and 
should go away within an hour. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 200 of 221 
 
 
 
 
5. Wash your hands after you apply the patch. Nicotine on your hands could get into your 
eyes and nose, and cause stinging, redness, or more serious problems. 
6. After wearing the patch for 4 hours call the study nurses at 443-740-2294 to tell them how 
you are responding.  If you do not call by 4:00 PM a member of the research team will contact 
you.  Continue wearing the patch for the entire day and evening. Remove the patch just before 
you go to bed, the night before your NIDA visit. 
7. On the morning of your NIDA visit, place a new patch on as soon as you wake up.  
 
If your patch comes off during the day: 
The patch generally sticks well to most people’s skin. However, a patch may occasionally 
come off. If your patch falls off during your abstinence period, re-apply as soon as possible and 
hold in place with medical tape.  
To prevent the patch from coming off, do not apply creams or lotions to the area on your 
skin where you will put the patch. Also, do not bathe, swim or shower during your test. 
 
If you get a minor skin rash: 
Use a topical salve (such as a hydrocortisone cream) around the patch. 
 
If you experience any worrisome side effect from the patch (listed below):  
Remove it at once and call Dr. Betty Jo Salmeron at 443-740-2651. Also, the study 
nurses can be reached at 443-740-2294 (available 24 hours every day). 
 
Side effects of the nicotine patch include: dizziness, headache, upset stomach, vomiting, 
diarrhea, redness or swelling at the patch site. 
 
Very rare serious side effects of the nicotine patch include: severe rash or swelling, 
seizures, abnormal heartbeat or rhythm, difficulty breathing. 
 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 201 of 221 
 
 
 
 
Appendix 6: Coping strategies 
Methods to help you cope with smoking withdrawal 
symptoms 
1. Reminding yourself of the positive consequences of not smoking (such as improved 
health and appearance, feelings of success and self-control, monetary savings) as well as 
the negative consequences of smoking (e.g., such as the smell on clothing, hands and 
breath, negative health consequences).  
 
 
 
 
 
2. Stay busy. Keep yourself busy with activities that do not involve smoking. For example, 
spending time with nonsmoking friends, going to the movies, taking long walks, cooking.  
 
3. Keep your hands active. Some smokers miss the tactile sensation of holding a cigarette. 
Find something you can hold and manipulate to help with this. Manipulating a stress ball 
or just a pen or pencil can help relieve the desire to hold a cigarette.  
 
4. Have healthy snacks on hand. You may find that your appetite increases when you stop 
smoking. Having healthy snacks available (e.g., carrot or celery sticks, sugarless candie 
or chewing gum – not nicotine gum) will help with this. Also, instead of taking a 
cigarette break, take a break and have a snack instead.   
 
5. Relaxation. Feeling tense or stressed will make it even more difficult for you to stop 
smoking. We have provided you with a 10min progressive muscle relaxation guide that 
you can use to help reduce tension, relieve stress and help you relax. Try doing this 10 
min relaxation tape when you are feeling particularly tense/stressed or particularly strong 
desire to smoke. 
 
Here are some progressive relaxation videos you can follow: 
http://www.youtube.com/watch?v=HFwCKKa--18 
http://www.youtube.com/watch?v=PYsuvRNZfxE 
http://www.youtube.com/watch?v=KmxfjjamcuY 
 
 
Exercise 
Make a list of reasons you want to quit smoking. Try not to only come up with negative 
consequences to be avoided, but also benefits to be made. From this list, pick the number 1 reason 
you want to quit smoking. This can function as a simple internal message of encouragement when 
you are finding the abstinence period difficult.  

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 202 of 221 
 
 
 
 
Appendix 7: Varenicline Toleration Assessment 
 
After participants start varenicline, a masters level therapist, LI, or study clinician will 
conduct a semi-structured interview to assess toleration of varenicline. The assessment will be 
conducted weekly, either in person or via phone, while participants are taking varenicline, and for 
two weeks after their last dose. The therapist will be extensively trained by the MAI on question 
administration.  If the interview reveals a possibility of significant side effects, the participant will 
be put in contact with the MAI for further evaluation.  The MAI will decide whether study pill 
administration needs to be discontinued and other action taken based on the severity of the 
symptoms.  
 
1) Since we last spoke or met, how have you been feeling? (open-ended question) 
2) Have you noticed any physical side effects [or, a change in the severity of any physical 
side effects], such as nausea, vomiting, dizziness, or headache? Anything else? 
3) Have you noticed a change in behavior that would be considered out of the ordinary for 
you? 
4) Do you feel more restless, wound-up, or agitated than usual? 
5) Do you feel more irritable or cranky than usual? 
6) Have you noticed an increase in feelings of depression, such as sadness, hopelessness, 
helplessness or worthlessness? 
8) Have you had thoughts of violence towards others? …acted aggressively towards 
someone? 
7) Have you had thoughts of death? …wished you were dead? …had thoughts of killing 
yourself? 
 
Participants will also be reminded to call if any issues should arise between assessments. 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 203 of 221 
 
 
 
 
Appendix 8: Take-home pill instructions 
 
You have been given a 2 week supply of a medication called Varenicline (Chanitx©). 
Varenicline is approved by the FDA to help people quit smoking.  This package contain 11 x 0.5mg 
Tablets and 42 x 1mg Tablets.  
 
Week 1: 
Days 1 to 3: Take 1 x 0.5mg pill each day in the morning.  
Days 4 to 7: Take 2 x 0.5mg pills each day. Take one in the morning and one in the evening.  
 
Week 2 to 12:  
Take 2 x 1mg pills each day.  Take one in the morning and one in the evening. 
 
How to take the pills: 
Pills should be taken at least 8 hours apart.  You should take each study pill with food and 
with a glass of water to minimize possible nausea.  
 
If you forget to take a study pill: 
If you forget to take a pill, simply leave the pill in its spot and skip to the next pill.  
 
Side effects: 
Common side-effects from varenicline are:  ●nausea  ●vomiting  ●difficulty sleeping  
●abnormal dreams  ●headaches, and   ●feeling tired or sleepy.  Other side effects could include:  
●change in appetite  ●stomach pain  ●gas  ●indigestion  ●constipation  ●dry mouth  ●diarrhea  
●blurred vision  ●sweating  ●hot flush  ●increased heart rate  ●changes in blood pressure  ●back 
and muscle pain  ●disturbances in attention  ●dizziness  ●fainting  ●restlessness  ●anxiety  
●depression  ●irritability and  ●agitation.  In rare cases heart problems, low blood sugar and 
serious changes in behavior and mood related to anxiety, depression, suicidal thinking, and strange 
behavior have been reported.  A complete list of varenicline related side effects can be found in the 
consent form. 
 
You will be asked about side-effects and how you are responding to varenicline during 
your weekly counseling visits.  
 
If you experience any worrisome side effects from the study pills: 
Immediately stop taking the study pills and contact the study Nurses or Physician. If you 
have any questions or concerns, call the Nurses at 443-740-2294 (available 24 hours everyday). 
If you have any issues that you would like to discuss with the study Physician contact Dr. Betty Jo 
Salmeron at 443-740-2651 (office). 
If you experience a sudden or drastic change in behavior or mood including severe 
agitation, depressed mood, suicidal thoughts or behaviors, stop taking the pills and contact Dr. 
Salmeron immediately.   
If you need to seek medical attention for any possible side effects tell your doctor about 
your participation in this study and that you might be taking varenicline. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 204 of 221 
 
 
 
 
Appendix 9: Treatment Preparation and Motivation to Quit 
Measures 
 
During treatment sessions, participants may be asked to repeat some of the questionnaires assessing 
motivation to quit in order to assess changes in motivation levels and/or specific issues of concern 
that should be addressed in session. 
 
1) Processes of Change (Prochaska, Velicer, DiClemente, & Fava, 1988). The Processes of 
change questionnaire is a self-report measure which assesses readiness to change smoking 
behavior. The scale includes 40 items which are each assessed on a 5-point likert scale from 
Never to Repeatedly. 
 
The following experiences can affect the smoking habits of some people. Think of any 
similar experiences you may be currently having or have had in the last month. Then rate the 
FREQUENCY of this event on the following five point scale.  
 
1 = Never 
2 = Seldom 
3 = Occasionally 
4 = Often 
5 = Repeatedly 
1. 
Special people in my life accept me the same whether I smoke or 
not. 
 
2. 
I see "No Smoking" signs in public buildings. 
3. 
I can be open with at least one special person about my experiences 
with smoking. 
 
4. 
I tell myself I can choose to smoke or not. 
 
5. 
Instead of smoking I engage in some physical activity. 
6. 
I recall articles with the problems of quitting smoking. 
7. 
I notice that public places have sections set aside for smokers. 
 
8. 
I recall information people have personally given me on the benefits 
of quitting smoking. 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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9. 
I am considering the belief that people quitting smoking will help to 
improve the world. 
 
10. 
I think about information from articles and advertisements on how to 
stop smoking. 
 
11. 
Remembering studies about illnesses caused by smoking upsets me. 
12. 
Other people in my daily life try to make me feel good when I don't 
smoke 1 2 3 4 5 
 
13. 
I tell myself I am able to quit smoking if I want to. 
14. 
I have someone who listens when I need to talk about my smoking. 
 
15. 
I remove things from my home that remind me of smoking. 
16. 
I tell myself that if I try hard enough I can keep from smoking. 
17. 
I recall information people have personally given me on how to stop 
smoking. 
 
18. 
I make commitments not to smoke. 
 
19. 
I reward myself when I don't smoke. 
 
20. 
I notice that nonsmokers are asserting their rights. 
21. 
I stop to think that smoking is polluting the environment. 
22. 
I can expect to be rewarded by others if I don't smoke. 
 
23. 
I keep things around my place of work that remind me not to smoke. 
24. 
I find society changing in ways that make it easier for the 
nonsmoker. 
 
25. 
I get upset when I think about my smoking. 
 
26. 
I find that doing other things with my hands is a good substitute for 
smoking. 
 
27. 
When I am tempted to smoke, I think about something else. 
28. 
I do something else instead of smoking when I need to relax or deal 
with tension. 
 
29. 
I remove things from my place of work that remind me of smoking. 
30. 
Warnings about the health hazards of smoking move me 
emotionally. 
 
31. 
Dramatic portrayals of the evils of smoking affect me emotionally. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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32. 
I react emotionally to warnings about smoking cigarettes. 
33. 
I am rewarded by others if I don't smoke. 
34. 
I consider the view that smoking can be harmful to the environment. 
 
35. 
I reassess the fact that being content with myself includes changing 
the smoking habit. 
 
36. 
I consciously struggle with the issue that smoking contradicts my 
view of myself as a caring and responsible person. 
 
37. 
I put things around my home that remind me not to smoke. 
38. 
My dependency on cigarettes makes me feel disappointed in myself. 
 
39. 
I am considering the idea that the world around me would be a better 
place without my smoking. 
 
40. 
I have someone whom I can count on when I'm having problems 
with smoking. 
 
 
2) Decisional Balance (Velicer, DiClemente, Prochaska, & Brandenburg, 1985).  The 
decisional balance questionnaire assesses the perceived pros and cons of smoking. The scale is a 
20 item self-report measure with each item assessed on a 5-point likert scale from Not Important 
to Extremely. 
The following statements represent different opinions about smoking. Please rate HOW 
IMPORTANT each statement is to your decision to smoke according to the following five point scale: 
  
1 = Not important 
2 = Slightly important 
3 = Moderately important 
4 = Very important 
5 = Extremely important     
     
1. 
Smoking cigarettes is pleasurable. 
 
2. 
My smoking affects the health of others. 
 
3. 
I like the image of a cigarette smoker. 
 
4. 
Others close to me would suffer if I became ill from smoking. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
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5. 
I am relaxed and therefore more pleasant when smoking. 
6. 
Because I continue to smoke, some people I know think I lack the 
character to quit. 
 
7. 
If I try to stop smoking I'll be irritable and a pain to be around. 
8. 
Smoking cigarettes is hazardous to my health. 
9. 
My family and friends like me better when I am happily smoking 
than when I am miserably trying to quit. 
 
10. 
I'm embarrassed to have to smoke. 
 
11. 
I like myself better when I smoke. 
 
12. 
My cigarette smoking bothers other people. 
 
13. 
Smoking helps me concentrate and do better work. 
 
14. 
People think I'm foolish for ignoring the warnings about cigarette 
smoking. 
 
15. 
Smoking cigarettes relieves tension. 
 
16. 
People close to me disapprove of my smoking. 
 
17. 
By continuing to smoke I feel I am making my own decisions. 
18. 
I'm foolish to ignore the warnings about cigarettes. 
 
19. 
After not smoking for a while a cigarette makes me feel great. 
20. 
I would be more energetic right now if I didn't smoke. 
 
3) Self-Efficacy/Temptation (Velicer, Diclemente, Rossi, & Prochaska, 1990). The self-
efficacy/Temptation model measures perceived self-efficacy to maintain abstinence across a 
variety of high-risk smoking situations. The measure is a 20 item scale where each item is a 
assessed on a 5-point likert scale from Not at all tempted to Extremely tempted. 
Listed below are situations that lead some people to smoke. We would like to 
know HOW TEMPTED you may be to smoke in each situation. Please answer the 
following questions using the following five point scale. 
 
1 = Not at all tempted 
2 = Not very tempted 
3 = Moderately tempted 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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4 = Very tempted 
5 = Extremely tempted      
1. 
At a bar or cocktail lounge having a drink. 
 
2. 
When I am desiring a cigarette. 
 
3. 
When things are not going the way I want and I am frustrated. 
4. 
With my spouse or close friend who is smoking. 
 
5. 
When there are arguments and conflicts with my family. 
6. 
When I am happy and celebrating. 
 
7. 
When I am very angry about something or someone. 
8. 
When I would experience an emotional crisis, such as an accident or 
death in the family. 
 
9. 
When I see someone smoking and enjoying it. 
 
10. 
Over coffee while talking and relaxing. 
 
11. 
When I realize that quitting smoking is an extremely difficult task 
for me. 
 
12. 
When I am craving a cigarette. 
 
13. 
When I first get up in the morning. 
 
14. 
When I feel I need a lift. 
 
15. 
When I begin to let down on my concern about my health and am 
less physically active. 
 
16. 
With friends at a party. 
 
17. 
When I wake up in the morning and face a tough day. 
18. 
When I am extremely depressed. 
 
19. 
When I am extremely anxious and stressed. 
 
20. 
When I realize I haven't smoked for a while. 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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4) Reasons for Quitting Scale (Curry, Wagner, & Grothaus, 1990). The Reasons for 
Quitting scale is a self-report measure which assesses endorsement of extrinsic and intrinsic 
reasons for quitting smoking. The measure contains 20 items which are each assessed on a 5-
point likert scale from Not at all true to Extremely true. 
 
What are your reasons for wanting to quit smoking at this time? Below is a list of reasons that 
smokers may have for quitting. Read each reason and decide how much it applies to you right now. Then 
circle ONE number for each reason. Remember, there are no “right” or “wrong” reasons for wanting to quit 
smoking. Any reason is a good one! 
 
0 Not at all 
1 A little true 
2 Moderately true 
3 Quite true 
4 Extremely true 
 
I WANT TO QUIT SMOKING:  
1. Because I am concerned that I will suffer from a serious illness if I don't quit 
smoking.........................0...........1............2............3...........4  
2. To show myself that I can quit smoking if I really want 
to..................0...........1............2............3...........4  
 
3. So that my hair and clothes won’t 
smell....................................................0...........1............2............3...........4  
4. Because my spouse, children, or other person I am close to will  
stop nagging me if I quit smoking......0...........1............2............3...........4  
 
5. Because I have noticed physical symptoms that smoking is  
hurting my health................................0...........1............2............3...........4  
 
6. Because I will like myself better if I quit 
smoking.......................................0...........1............2............3...........4  
7. So that I will save money on smoking related costs such as  
dry cleaning........................................0...........1............2............3...........4  
 
8. Because someone has given me an ultimatum (made a threat) to  
quit......................................................0...........1............2............3...........4  
 
9. Because I can graphically picture  the effects that smoking has on  
my body..............................................0...........1............2............3...........4  
10. So that I can feel in control of my 
life..................................................0...........1............2............3...........4  
11. Because I won’t burn holes in  
clothing or furniture............................0...........1............2............3...........4  
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
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12. Because I will receive a special  
gift if I quit..........................................0...........1............2............3...........4  
 
13. Because I have known other people who have died from serious illnesses that 
were caused by 
smoking...............................................0...........1............2............3...........4  
 
14. Because quitting smoking will prove that I can accomplish other  
things that are important to me.........0...........1............2............3...........4  
 
15. Because I want to save money that I spend on 
cigarettes..................0...........1............2............3...........4  
 
16. Because people I am close to will be upset with me if I don’t 
quit..........0...........1............2............3...........4  
17. Because I am concerned that smoking will shorten my 
life..............0...........1............2............3...........4  
 
18. To prove to myself that I am not addicted to 
cigarettes........................0...........1............2............3...........4  
 
19. So that I won’t have to clean my house or car as 
often..........................0...........1............2............3...........4  
 
20. Because I will receive a financial reward for quitting (money from a friend or 
family  
member, bonus from work, etc.)........0...........1............2............3........... 
 
 
5) Smoking Cessation Motivation Assessment – a semi-structured interview to assess/ 
confirm the stage of change and motivation level for participants interested in smoking cessation 
protocols. Follow up questions and a brief discussion of readiness to quit may ensue. Some items 
from this questionnaire may be repeated several times throughout the protocol. 
 
1. a) Are you seriously thinking of quitting smoking? 
If yes:  
b) Of the following choices, what would be a more accurate description of your 
readiness to attempt quitting smoking: within the next 30 days\ within the next 6 
months?  
Notes: 
 
2. Have you tried to quit before? Yes/No 
If yes: 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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a. How many times and over what period? 
_______________________________________ 
b. How long did you remain abstinent and why did you start smoking 
again?____________________________________________________________
______________________________ 
 
3. On a scale of 0-10 with 0 being not at all and 10 being extremely, how important to you 
is quitting smoking? 
_______________________________________________________________ 
Notes: 
 
4. Do other people in your home smoke? Yes/No 
Notes: 
 
5. Do you have friends at work who you take smoke breaks with? Yes/No 
Notes: 
 
6. Do you have friends outside of work you smoke with? Yes/No 
Notes: 
 
7. In what situations do you enjoy smoking the most? 
________________________________________________________________________
___________________________________________ 
 
8. On a scale of 0-10 with 0 being not at all and 10 being extremely, how interested right 
now are you in quitting? 
_________________________________________________________________ 
 
9. On a scale of 0-10 with 0 being not at all and 10 being extremely, how ready are you to 
quit smoking within the next month? 
__________________________________________________ 
 
10. On a scale of 0 to 10 with 0 being not at all and 10 being extremely, how confident are 
you that you could quit smoking within the next month? 
________________________________________ 
Notes: 
GeneralComments:_____________________________________________________________
______________________________________________________________________________
______________________________________________________________________________
______________________________________________________________________________
_____________________ 
 
Questions that may be repeated throughout treatment: 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
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1. On a scale of 0-10 with 0 being not at all and 10 being extremely, how important to you 
is quitting smoking? 
_______________________________________________________________ 
Notes: 
 
2. On a scale of 0-10 with 0 being not at all and 10 being extremely, how interested right 
now are you in quitting? 
_________________________________________________________________ 
Notes: 
 
3. On a scale of 0-10 with 0 being not at all and 10 being extremely, how ready are you to 
quit smoking? 
______________________________________________________________________ 
Notes: 
 
4. On a scale of 0 to 10 with 0 being not at all and 10 being extremely, how confident are 
you that you could quit smoking within the next month? 
________________________________________ 
Notes: 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Appendix 10: Treatment Materials 
Participants may be given these treatment materials during counseling sessions or as 
take home materials.  
 
Please see attached documents Appendix10.pdf 
 
Appendix 10 (Treatment Session and Take-Home Materials): Table of Contents  
1. Tips for Successful Quitting 
2. Putting a Stop to Smoky Thinking 
3. Deep Breathing Relaxation Exercise 
4. What Are Smoking Triggers 
5. Triggers for Smoking 
6. Managing Triggers for Smoking 
7. Managing Triggers for Smoking Worksheet 
8. Nonsmoking Game Plan: Coping with High-Risk Situations 
9. Nonsmoking Game Plan: Coping with High-Risk Situations Worksheet 
10. Nonsmoking Game Plan: Feeling Badly if You Slip 
11. Preparing for Quit Day 
12. Your Quit Day (3 pages) 
13. Instructions for Mini-Dose Relaxation 
14. Nonsmoking Game Plan: Lifestyle Change 
15. Why Quit Smoking – Revisited 
16. Social Support for Nonsmoking 
17. Weekly Task Worksheet 
18. Quitting for Keeps: Remaining a Nonsmoker 
19. All-Purpose Coping Plan 
20. Combating Triggers 
21. Coping with Cravings and Urges 
22. Decisional Balance Worksheet 
23. Goals Worksheet 
24. Reminder Sheet for Problem-Solving 
25. Seemingly Irrelevant Decisions 
26. Self-Monitoring Your Smoking Behavior 
27. Beat Boredom 
28. Burns You Up 
29. Coping with Quitting – CB Strategies 
30. What Happens When You Quit 
31. NCI – How to Handle Withdrawal and Triggers 
32. Functional Analysis 
33. Quit Day Ceremony 
34. 
Abstinence Coping Strategies 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Appendix 11: Appointment Cards 
 
Front 
Back 
 
Nicotine Treatment Studies 
    Contact Person: Kim Slater, M.Ed., NCC 
    Phone: 443-740-2252 
    Email: QuitSmoking@mail.nih.gov 
                                        
                                           
             
 
 
Appointment Information: 
 
      Date:___________  Time: _________ 
      Study #:___________  Session #:________ 
MD Hotline - 24 Hours/7 Days Week: 
1-800-Quit-Now ~ www.smokingstopshere.com 
NIDA Nursing Phone: 443-740-2294 
 
 
Nicotine Treatment Studies 
    Contact Person: ____________________ 
    Phone: ____________________________ 
    Email: QuitSmoking@mail.nih.gov 
                                        
                                           
             
 
 
 
Appointment Information: 
 
      Date:___________  Time: _________ 
      Study #:___________  Session #:________ 
MD Hotline - 24 Hours/7 Days Week: 
1-800-Quit-Now ~ www.smokingstopshere.com 
NIDA Nursing Phone: 443-740-2294 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
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Appendix 12: Nicotine Patch Instructions for tDCS Arm 
Read ALL the instructions before you begin.  
 
Do not smoke any cigarettes while you are wearing the patches. Since the nicotine patch works by delivering 
small doses of nicotine into the body, smoking may cause harmful levels of nicotine in your body. 
 
Nicotine Tolerance Test Patch 
• 
The nurse will apply a nicotine patch for you on orientation day to make sure you tolerate it. 
• 
Update the staff if you experience any discomfort with wearing the patch. 
• 
The nursing staff will remove the patch at the end of the orientation session.  
Call the study nurses at 443-740-2294 or Dr. Salmeron at 443-740-2651 if you experience any problems after 
you leave NIDA.  
 
Study Patches (1 and 2) 
You will receive 2 take-home patch sets. One of these patches contains nicotine, and the other contains a placebo 
(no nicotine). The content of the patches is blinded, meaning the packaging does not reveal the true content of 
the patch. These patches will be worn on the day of your imaging visit. 
 
Your abstinence period begins 12 hours before your next NIDA visit (e.g., 8pm Sunday night if your visit is 
at 8am on Monday morning). Smoke a cigarette after dinner, before you commence the abstinence period, if 
this is usual for you. You will receive a phone call from a study investigator before your abstinence period 
begins to ensure that you remember and understand the abstinence procedure.  
 
A team member will tell you what time to place the patch so that you are wearing it for about two hours before 
your scan. To place the patch, follow the instructions outlined on the next page.  
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
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How to apply the nicotine patch: 
 
1. Do not remove the patch from its sealed protective pouch until you are ready to use it. 
 
2. Choose a non-hairy, clean, dry area of skin. We recommend placing it near where the nurse applied 
your toleration patch. Do not put the patch on skin that is burned, broken out, cut, or irritated in any 
way. Make sure your skin is free of lotion and soap before applying the patch. 
 
3. A clear, protective liner covers the sticky backside of the patch – the side that will be put on your skin. 
The liner has a slit down the middle to help you remove it from the patch. With the sticky backside 
facing you, pull half the liner away from the patch starting at the middle slit, as shown in the illustration 
below. Hold the patch at one of the outside edges (touch the sticky side as little as possible), and pull 
off the other half of the protective liner.   
 
 
 
4. Immediately apply the sticky side of the patch to your skin. Press the patch firmly on your skin with 
the palm of your hand for at least 10 seconds. Make sure it sticks well to your skin, especially around 
the edges. Mild itching, burning or tingling is normal and should go away within an hour. 
 
5. Wash your hands after you apply the patch. Nicotine on your hands could get into your eyes and nose, 
and cause stinging, redness, or more serious problems. 
 
 
 
 
 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
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If your patch comes off during the day: 
The patch generally sticks well to most people’s skin. However, a patch may occasionally come 
off. If your patch falls off during your abstinence period, re-apply as soon as possible and hold in 
place with medical tape.  
 
To prevent the patch from coming off, do not apply creams or lotions to the area on your skin where 
you will put the patch. Also, do not bathe, swim or shower during your test. 
 
If you get a minor skin rash: 
Use a topical salve (such as a hydrocortisone cream) around the patch. 
 
If you experience any worrisome side effect from the patch (listed below):  
Remove it at once and call Dr. Betty Jo Salmeron at 443-740-2651. Also, the study nurses can 
be reached at 443-740-2294 (available 24 hours every day). 
 
Side effects of the nicotine patch include: dizziness, headache, upset stomach, vomiting, diarrhea, 
redness or swelling at the patch site. 
 
Very rare serious side effects of the nicotine patch include: severe rash or swelling, seizures, 
abnormal heartbeat or rhythm, difficulty breathing. 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Appendix 13: tDCS Stimulation Blinding Questionnaire 
(Adapted from Fertonani et al 2015, O’Connell et al 2012, Richardson 2016)  
 
Questions for Participant 
 
Blinding 
 
1. 
 Which treatment do you believe you received? If you don’t know, please provide your best (or 
random) guess. 
 
a) 
Active  
b) 
Placebo (sham stimulation) 
 
 
2. 
On a scale of 0 to 10 -- 0 being not confident at all and 10 being completely confident -- how 
confident are you that you received your selection? ______ 
 
Side Effects 
 
Did you experience any discomfort or annoyance during the tDCS stimulation? Please answer the 
following questions regarding the different sensations and indicate the degree of intensity of your 
discomfort according to the following scale: 
 
None = I did not feel the describe sensation (0) 
Mild = I mildly felt the described sensation (1) 
Moderate = I felt the described sensation (2) 
Considerable = I felt the described sensation to a considerable degree (3) 
Strong = I strongly felt the described sensation (4) 
 
1. Itching 
2. Pain 
3. Burning 
4. Warmth/Heat 
5. Pinching 
6. Metallic/Iron taste 
7. Fatigue 
8. Other: _____ 
 
When did the discomfort begin?  
At the beginning of the session, in the middle of the session, towards the end of the session 
 
How long did it last?  
It stopped quickly, it stopped in the middle of the session, it stopped at the end of the session 
 
How much did these sensations affect your performance?  
Not at all, slightly, considerably, much, very much 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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Identify whether these sensations were located over the head or in a different location:  
On the head ___, Other ____ 
 
 
Questions for Investigator 
 
1. 
 Which treatment do you believe this participant received? 
 
a) Active (full dose stimulation) with anode over the dlPFC and cathode over the vmPFC 
b) Active (full dose stimulation) with anode over the vmPFC and cathode over the dlPFC 
c) Active (full dose stimulation) but I don’t know which polarity 
d) Placebo (sham stimulation) 
e) Don’t know 
 
2. 
If you answered “Don’t know” above, can you please provide your best (or random) guess of a 
treatment the participant received anyway? 
  
a) Active (full dose stimulation) with anode over the dlPFC and cathode over the vmPFC 
b) Active (full dose stimulation) with anode over the vmPFC and cathode over the dlPFC 
c) Active (full dose stimulation) but I don’t know which polarity 
d) Placebo (sham stimulation) 
 
3. 
On a scale of 0 to 10 -- 0 being not confident at all and 10 being completely confident 
-- how confident are you that the participant received your selection? 
 
______ 
 
Side effects 
Please report any adverse event/problem (skin irritation, headache, scalp pain, dizziness, or others, please 
specify) that occurred and rate the event/problem on a scale from 1 to 4 as described above. 
 
Additional comments: 
 
Appendix 14: Recruitment Materials 
 
See attached pdf of advertisements and landing pages. Recruitment through the NIDA 
Office of the Clinical Director will be done by a general NIDA-IRP recruitment contractor. Under 
certain circumstances, materials may be developed by other contractors as well. Alternatively, we 
may develop our own ads or contract these services ourselves. All advertising methods will 
comply with the most current regulations (NIH and OHSRP SOPs and guidelines, as well as FDA 
guidelines for FDA-regulated research) and the NIDA policy on recruitment materials. Please see 
attached pdf of recruitment materials which includes the following: advertisements, landing pages 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
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and alternative phrasing and photos to be substituted for the purpose of A/B testing of best 
performing ads. 
The recruitment contractor may alter the ads in minor ways without prospective IRB 
review and approval; see below for a list of these types of changes. Other than that, modifications 
to these materials will be submitted to the IRB for approval as protocol amendments. 
The following changes may be made to recruitment materials with no prospective review 
and approval by the IRB (see next section for further explanation about each bullet point): 
• 
Contact phone numbers 
• 
Color change to graphics 
• 
Overall size, but all content will be increased or decreased proportionately 
• 
Addition of tear-off phone number tags to an approved ad or flyer 
• 
Color or font of the text, but any color change will replace all the text of a certain 
color. No new emphasis will be created. 
All of these changes will be made to the most recently approved recruitment materials 
and submitted to the IRB annually at the time of continuing review. Materials will only be posted 
in approved locations. 
 
Contact phone numbers: Phone numbers can vary for several reasons, including A/B 
efficacy comparison testing on ads and having phone numbers vary by placement, image, style, or 
wording. This allows us to know which ad or ad placement drew in the phone call for screening 
since the phone number dialed is tracked. As such, we would like the flexibility to change the 
phone number(s) listed on any approved ad without prior approval to make this change. 
 
Color change to graphics: Due to requirements from organizations where our recruitment 
material is placed or A/B testing, we may need to or want to change the colors in the graphics 
from color to black and white or vice versa, or change a red border to a blue border, etc. Any 
changes to color in the graphics will not create, amplify, or diminish the emphasis in the original 
approved materials. 
 
Overall size: We may need to re-size materials to fit the parameters of the hosting 
organization (e.g, must be 5” x 7” but the originally approved material was 8.5” x 11”, quarter 
page newspaper ad to a full page newspaper ad, newspaper format size to a bus ad format size) or 
make an ad from a flyer to a larger format poster for display at an outreach event. We will ensure 
that all the originally approved content remained in the same proportions when the overall 
document size was reduced or enlarged. 
 
Addition of tear-off phone number tags: We may add or remove tear-off contact 
information to approved ads and flyers. This allows us to be flexible in the type of material we 
provide to organizations to post for us. 
 
Color or font of the text: We may change the color of font in an ad as long as it is all one 
color to another color (e.g., all blue text changed to green text, all red text to black text). We will 
not create new emphasis (e.g., we will not change only part of a text field to a color like making 
the word free blue in what was all black text, we will not make a payment amount a color to 
highlight it when it was approved as black or the same color as the surrounding text) or remove 
emphasis. We may change the font of the entire text to meet requirements for placement, for A/B 
testing, for readability, or other placement needs (e.g., change the font through an entire ad from 

Protocol 12-DA-N474: Identifying neurobiological mechanisms that underlie acute nicotine withdrawal and drive early relapse in 
smokers.                                                                                                                                                        
 
                                                                                                                                                                       Date: March 2, 2022  
Principal Investigator: Amy C. Janes, Ph.D.                                                                                                                
 
 
 
CNS IRB Protocol Template (rev. 16Jun11) 
 
 
 
 
 
 
 
page 221 of 221 
 
 
 
 
Calibri to Times New Roman). We will not add or remove italics, bold, or any other emphasis 
without prior approval. 
 
Changes outside of the type listed above will be made by making an amendment to the 
protocol and its recruitment materials. We will not make changes that do not fit within the 
guidelines above.