Effects of the Longevity Diet and the Fasting-mimicking Diet on Body Composition, Risk Factors for Age-related Diseases
Conditions: Overweight, Obesity, Metabolic Syndrome, Autoimmune Diseases, Inflammatory Disease, Health, Subjective
Sponsor: Fondazione Valter Longo

 
 
Retrospective study of the effects of the longevity 
diet and the fasting-mimicking diet on body 
composition, risk factors for age-related diseases
13/03/2025

FONDAZIONE VALTER LONGO ONLUS ò C.F 95196780100 ò P.IVA 025666109990 ò IBAN: IT29V0538731870000047148247 ò SEDE LEGALE: VIA 
BORGOGNA, 9, 20122, MILANO, ITALY ò INFO@FONDAZIONEVALTERLONGO.ORG ò WWW.FONDAZIONEVALTERLONGO.ORG 
 
 
 
 
Thank you for reaching out to Valter Longo Foundation, and the affiliated specialty clinic 
with an expert dietary and scientific team specializes in cutting edge science-based 
nutrition and fasting protocols to prevent and manage major chronic diseases such as 
Cancer, Heart, Neurodegenerative and Autoimmune Diseases, Diabetes, and extend 
healthy longevity. 
 
Our clinic offers appointments, which involve providing nutritional counseling and 
Individualized Nutritional Protocols based on evidence-based study. These appointments 
are with our Registered Dietitians and Molecular Biologist. Each individual patient case is 
different, and therefore, cases can be reviewed and discussed by the Dietitian and 
Biologist in consultation. 
 
The information needed to set up an initial consult is provided to us by you, via the 
attached forms. Feel free to add any additional information you feel is relevant to you, 
including medical history or recent lab work or consultation notes. 
Once we receive these forms, we can move forward in setting up an appointment. 
Please 
complete 
the 
forms 
attached, 
and 
email 
back 
to: 
nutrizionisti@fondazionevalterlongo.org 
 
Thank you, and do not hesitate to reach out if you have any questions! 
 
Valter Longo Foundation 
www.fondazionevalterlongo.org 
nutrizionisti@fondazionevalterlongo.org 
 
We welcome you and look forward to meeting you soon! 
13/03/2025

FONDAZIONE VALTER LONGO ONLUS ò C.F 95196780100 ò P.IVA 025666109990 ò IBAN: IT29V0538731870000047148247 ò SEDE LEGALE: VIA 
BORGOGNA, 9, 20122, MILANO, ITALY ò INFO@FONDAZIONEVALTERLONGO.ORG ò WWW.FONDAZIONEVALTERLONGO.ORG 
 
 
 
Treatment Contract - Policies and Limitations 
 
The Fondazione Valter Longo (<FVL=) provider-patient relationship is extremely important 
to us, and communication is critical to maintaining this relationship in good standing. This 
Agreement explains howwe will work together. This Agreement is made between FVL 
and you (<you= or <Patient=). 
FVL through its Registered Dietitians and Molecular Biologists offers nutrition guidance 
and recommendations for lifestyle management nutrition guidance of disease in exchange 
for certain fees paid by you according to the terms and conditions described below and 
pursuant to article. 3 of the italianlaw n. 396/1967, and This assignment begins from the 
signing of this form and is intended as conferred until the end of the professional service 
requested. 
 
AGREEMENT 
 
PATIENT: As used in this Agreement, the term Patient means a person for whom 
Fondazione Valter Longo (FVL) will provide services. FVL reserves the right to accept or 
decline patients based upon provider9 capability to appropriately manage the lifestyle 
medicine needs of its patients. 
 
CONSULTATION SERVICES: FVL will see you for consultation with recommendations 
for nutrition andlifestyle management (diet, nutrition, physical activity, stress management, 
and sleep) of your conditions.All patients must have a Primary Care Provider who is the 
primary physician managing their care and with whom FVL9s provider can communicate 
and coordinate care. 
 
Patients must share and discuss the recommendations for lifestyle management 
(diet, fasting, nutrition, mimicking fasting, physician activity, stress management, 
and sleep) received from anyhealthcare provider at Fondazione Valter Longo with 
their personal doctors and/or primary physician and/or specialist that are managing 
their care prior to moving forward with recommendations/direction. Prior to moving 
forward with any recommendations/direction received from any healthcare provider 
at Fondazione Valter Longo, patients must obtain the approval of their Primary Care 
Provider and/or Specialist (if applicable) managing their care. Patients hereby 
acknowledge they will not hold the Foundation, its clinic, employees or healthcare 
providers (<HCP=s) responsible if they do not follow the instructions as stated in 
this Agreement and/or move forward without their Primary Care Provider and/or 
Specialist approval. 
 
EXCLUDED SERVICES: Our HCPs or providers are not Primary Care Providers (PCP) 
and do not provide primary care services. We do not accept responsibility for ongoing 
management of your entire care, writing/refilling prescriptions medications, nor providing 
urgent care services. Our meeting is a consultation and does not constitute an agreement 
on FVL9s part to treatment or ongoing treatment. 
13/03/2025

FONDAZIONE VALTER LONGO ONLUS ò C.F 95196780100 ò P.IVA 025666109990 ò IBAN: IT29V0538731870000047148247 ò SEDE LEGALE: VIA 
BORGOGNA, 9, 20122, MILANO, ITALY ò INFO@FONDAZIONEVALTERLONGO.ORG ò WWW.FONDAZIONEVALTERLONGO.ORG 
 
 
 
AVAILABILITY: Our services, including lifestyle medicine or lifestyle management, 
do not include, and should not be considered to include, emergecy or urgent 
medical or psychiatric care. For medical or psychiatric emergencies, you must call an 
emergency or urgent care provider. For urgent medical problems you must seek care from 
your primary care physician, an urgent care clinic, or emergency department setting. We 
will make every effort to address your lifestyle medicine or lifestyle management concerns. 
Please allow 5-7 working days for us to respond to your questions. 
 
HEALTHCARE PROVIDER REFERRAL NOTE: 
In order for us to work directly with you, we would prefer to receive from your current 
healthcare provider (either your primary care physician, oncologist, endocrinologist, or 
other core healthcare provider) a referral note or prescription form prior to your initial 
appointment for nutrition guidance. 
 
CONFIDENTIALITY: FVL keeps records of appointments and the content of the 
appointments. They arein a secure electronic medical record system. Information you 
disclose will be kept confidential, unless you specifically authorize its release or unless 
the disclosure or release is permitted or required under State or Federal applicable law or 
regulation or professional standards of practice or conduct. Specifically, your right to 
confidentiality may not be maintained if there is reasonable concern of: 1) abuseor neglect 
of a child, dependent or elder adult, 2) danger of harm to yourself or to others, 3) grave 
disability (i.e., inability to care for yourself), 4) as required by law or regulation, or 5) 
pursuant to legal proceedings. 
13/03/2025

FONDAZIONE VALTER LONGO ONLUS ò C.F 95196780100 ò P.IVA 025666109990 ò IBAN: IT29V0538731870000047148247 ò SEDE LEGALE: VIA 
BORGOGNA, 9, 20122, MILANO, ITALY ò INFO@FONDAZIONEVALTERLONGO.ORG ò WWW.FONDAZIONEVALTERLONGO.ORG 
 
 
 
Your Privacy Rights. 
FVL will adhere to its obligations regarding your privacy rights as identified in FVL9sPrivacy 
Disclaimer. Please make sure to read the FVL Privacy Disclaimer carefully to 
understand your privacy rights, our obligations related to your privacy, and the 
circumstances when we may be permitted or required to share, use, disclose, or release 
your information. 
 
PHI Release Authorization: 
 
I, 
(patient's name) authorize FVL to disclose or 
release my protected health information, including my medical information, lab tests and 
results, and medical chartsand records to Professor Valter Longo Ph.D. and Nutritional 
Biologist Romina Cervigni Ph.D. for purposes of review of such information and 
consultation with FVL or Create Cures Foundation and its health care providers regarding 
my treatment or plan of care. 
 
Signature 
Date   
 
 
METHODS OF COMMUNICATION: You acknowledge that FVL communications may 
include email, fax, mobile application (<app=), telemedicine, telehealth, text messaging, 
telephone, and cell phone, and suchcommunications by their nature cannot be guaranteed 
to be secure or confidential. If you initiate a conversation in which you disclose 
personal information or protected health information, then you authorize the 
practice, FVL, to communicate with you regarding the information or PHI in thesame 
manner or format. Please be aware that transmitting confidential health information by 
email or text has a number of risks and we advise use of the Patient Portal provided as 
much as possible. We cannot guarantee the security or confidentiality of internet 
communications and are not liable for improperdisclosure of confidential information not 
caused by gross negligence or wanton misconduct on the part of FVL, or breaches of 
confidentiality caused by the patient. Your signature below signifies your acceptance of 
these terms. 
 
INSURANCE/BILLING/CO-PAYS: You are responsible for knowing your insurance 
benefits. Nutrition consultation and follow-ups, and some treatments/procedures/labs may 
not be covered by healthinsurance plans. Covered procedures vary with each insurance 
company. If you have a specific concern regarding your coverage, contacting your 
insurance company is the most assured way of checking coverage information. 
 
Patient is responsible for payment in full of all amounts billed by FVL for or related to its 
services providedto Patient. Patient will be responsible for any amount not paid by his 
eventual insurance. 
13/03/2025

FONDAZIONE VALTER LONGO ONLUS ò C.F 95196780100 ò P.IVA 025666109990 ò IBAN: IT29V0538731870000047148247 ò SEDE LEGALE: VIA 
BORGOGNA, 9, 20122, MILANO, ITALY ò INFO@FONDAZIONEVALTERLONGO.ORG ò WWW.FONDAZIONEVALTERLONGO.ORG 
 
 
 
CANCELLATION POLICY: We understand that emergencies arise, however, 
appointments are pre- arranged, and it is patient9s responsibility to keep or cancel your 
appointment with a minimum of 48 hours9 notice in advance. You will be charged for 
appointments which are not cancelled within this timeframe. 
 
VITAMINS and SUPPLEMENTS: Because some nutrients and vitamins should not be 
taken without continued monitoring, and new research is occurring in this field every day, 
supplement and vitamin recommendations shall expire 6 months from the initial 
recommendation unless otherwise discussed. 
 
 
PATIENT ARBITRATION CLAUSE: 
 
It is understood that any dispute as to medical malpractice, that is as to whether any 
medical services rendered under this contract were unnecessary or unauthorized or were 
improperly, negligently or incompetently rendered, will be determined by submission to 
arbitration in Italy as provided by Italian law, and not by a lawsuit or resort to court process 
except as Italian law provides for judicial review of arbitration proceedings. Both parties to 
this contract, by entering into it, are giving up their constitutional right to have any such 
dispute decided in a court of law before a jury, and instead are accepting the use of 
arbitration. 
Our goal is to provide medical care to our patients in a way that will avoid disputes. We 
know that most problems occur as a result of miscommunication. If you have concerns 
about your care, please discuss them with us. 
 
 
Please understand that by agreeing to the arbitration clause you are specifically 
giving up your right to litigate any Dispute in court along with the right to have 
your case or Dispute tried before a jury. 
 
NOTICE: BY SIGNING THIS CONTRACT YOU ARE AGREEING TO HAVE ANY ISSUE 
OF MEDICALMALPRACTICE DECIDED BY NEUTRAL ARBITRATION IN ITALY AND 
YOU ARE GIVING UP YOURRIGHT TO A JURY OR COURT TRIAL. 
 
I, 
(patient name) have read, review 
and 
understand the foregoing Patient Arbitration Clause and I hereby agree that all Disputes 
between me andFVL shall be decided by binding arbitration according to the terms of the 
Italian law. I specifically agreethat all Disputes as well as all gateway issues, including 
without limitation any issue related to whether any Dispute must be arbitrated, shall be 
delegated exclusively to, and decided exclusively by, the arbitrator in binding arbitration. 
 
Signature 
Date   
 
13/03/2025

FONDAZIONE VALTER LONGO ONLUS ò C.F 95196780100 ò P.IVA 025666109990 ò IBAN: IT29V0538731870000047148247 ò SEDE LEGALE: VIA 
BORGOGNA, 9, 20122, MILANO, ITALY ò INFO@FONDAZIONEVALTERLONGO.ORG ò WWW.FONDAZIONEVALTERLONGO.ORG 
 
 
 
TERMINATION: Both you and FVL shall have the absolute and unconditional right to 
terminate the Agreement, without cause. Notwithstanding any other provision of this 
Agreement, if your decision to terminate this agreement, or take other action related to 
FVL or its provider, is based on a grievance withone or more of our providers, you will give 
them an opportunity (not less than 15 days from the date younotify FVL or the provider of 
your grievance) to make it right, to cure the issue or problem giving rise to your grievance. 
If FVL or the provider is unable to cure or otherwise resolve your grievance, you may 
terminate this agreement by providing written notice of termination or intent to take other 
action. If FVL elects to terminate this Agreement, FVL will provide you thirty (30) days 
written notice, and you should follow-up with your Primary Care Provider. 
FVL has a right to determine whom to accept as a patient, just as you have the right to 
choose your physician. There are certain circumstances in which FVL may choose to 
terminate this Agreement. Such circumstances may include, but are not limited to the 
following: (1) You fail to pay fees and charges whenthey are due, (2) You fail to sign the 
arbitration agreement, (3) You have performed an act that constitutesfraud, (4) You fail to 
adhere to the recommended treatment plan, (5) You are disruptive, abusive, or present 
an emotional or physical danger to the staff or other patients of FVL. 
 
NON-DISCRIMINATION: FVL and its providers will not discriminate against you, or 
terminate this Agreement, on the basis of sex, race, color, religion, ancestry, national 
origin, disability, medicalcondition, genetic information, marital status, sexual orientation, 
citizenship, primary language, immigration status, or any other protected status. 
 
NO WAIVER: No waiver of a breach of any provision of this Agreement will be construed 
to be a waiver of this Agreement or any right of the non-breaching party under this 
Agreement, whether of a similar or different nature, and no delay in acting with regard to 
a breach shall be construed as a waiver of that breach or any right of the non-breaching 
party under this Agreement. 
 
LIMITS OF SERVICE: We do not provide primary care, psychiatric, emergency or urgent 
care, disability or workers compensation evaluations and treatments. If you have not 
received services from our office for a continual period of 12 months, and you request to 
start services again, you will be considered a newpatient. 
 
MISCELLANEOUS: This Agreement shall be governed by, and construed in accordance 
with, the laws of the Italy, and to the extent not preempted, the substantive laws of Italy. 
Should any provision of this Agreement be declared or determined to be invalid or illegal, 
the validity of the remaining provisions shall not be affected, and the invalid or illegal 
provision shall be deemed not to be a part of this Agreement and will be governed in 
accordance with the Italian laws.This document contains the entire agreement between 
the parties with respect to the subject matter of this Agreement. Any failure to enforce any 
provision of this Agreement will not constitute a waiver of thatprovision or of any other 
provision. This Agreement may not be amended, nor any obligation waived, except by a 
writing signed by both parties. 
13/03/2025

FONDAZIONE VALTER LONGO ONLUS ò C.F 95196780100 ò P.IVA 025666109990 ò IBAN: IT29V0538731870000047148247 ò SEDE LEGALE: VIA 
BORGOGNA, 9, 20122, MILANO, ITALY ò INFO@FONDAZIONEVALTERLONGO.ORG ò WWW.FONDAZIONEVALTERLONGO.ORG 
 
 
 
 
 
CONSENT TO TREAT 
 
I, 
 
(patient name) born 
in 
  
, on 
located 
in 
  
, at 
, with have 
readand understand the foregoing and consent to evaluation and treatment 
 
 
Name (Print)  
 
 
Signature   
 
 
Date   
 
13/03/2025

1 
 
 
 
 
 
 
 
 
PRIVACY 
DISCLAIMER 
[ART. 12, REG. (UE) 679/2106] 
 
Dear Sir or Madam, 
we invite you to carefully read the contents of the present privacy disclaimer, prepared by 
FONDAZIONE VALTER LONGO ONLUS with the purpose of describing use, disclose, or release of 
your medical information or protected health information and how you can get access to this 
information and with respect to the Regulation (EU) 2016/679. Please review it carefully. 
 
1 
CONTROLLER 
 
 
 
 
 
2 
DATA PROCESSED 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
3 
 
 
FONDAZIONE VALTER LONGO ONLUS 
VAT 02566610990 
Registered Office: Via Borgogna, 9 - Milano 
Privacy Office: Via Belgirate, 15 - Milano 
privacy@fondazionevalterlongo.org 
 
 
DATA PROVIDED 
In order to process your health information, medical records and results to 
Fondazione Valter Longo Onlus (hereinafter <Company=), we will ask you to submit 
some information. 
In particular, you may be asked to provide the following information (the following 
list is not comprehensive but is indicated merely as an example): your name and 
surname, your contact information (such as address, telephone number, email), 
data relating to the chosen payment method. 
 
Granting such information together is required in order to process the professional 
service requested to the Foundation; the failure to communicate such data may 
determine the impossibility to proceed. 
 
PARTICULAR CATEGORIES OF PERSONAL DATA 
The Company generally avoids to collect and process particular categories of 
personal data. Among those categories are included data that reveal your ethnic or 
racial origin, your political position, your religious or philosophical convictions, or 
your trade union affiliation or genetic data, biometric data aimed to identify 
uniquely a natural person, data on wealth or sexual life or sexual orientation of the 
person. 
 
Whenever, in specific cases, the Company should collect or anyway process 
particular categories of personal data, in order to achieve the purposes mentioned 
below, we will always process such data in accordance with dispositions of law and 
with the requirements as indicated in the present privacy disclaimer. 
 
 
The Company will process your data pursuant to Articles 6 and 9 of the Regulation 
(EU) 679/2016 and on the basis of the consent under Article 7: 
13/03/2025

2 
 
 
 
 
 
LEGAL BASIS FOR THE 
PROCESSING 
a. 
CONSENT - [art. 6, (1)(a), art. 7, and, if applicable, art. 9, (2)(a)] - the 
Company will process certain data solely upon your prior, free and express 
consent. You9ll have the faculty to withdraw your consent at any time with 
effect for the future. 
b. 
PROCESS YOUR SERVICE - [art. 6, (1) (b)] - in order to process the professional 
service with the Company, we need to have access to certain data. 
c. 
PROTECTION OF LEGITIMATE   INTERESTS   PURSUED   -   [art.   6,   (1)(f)] -   the 
Company will process certain data in order to protect its interests or third 
parties interests. Such principle, however, shall be applied except where 
such interests are overridden by your interests. 
 
 
4 
PURPOSES OF DATA 
PROCESSING 
The Company will process your data only for the purposes permitted by the law. 
This regards the following purposes: (i) purposes prior approved by you; (ii) data 
processing in order to process your donation; (iii) fulfillment of legal duties to 
which we are subject (included requirements set forth by the relevant Authorities); 
(iv) protection of our legitimate interests or of third parties legitimate interests, 
except where such interests are overridden by your interests; (v) exercise of our 
rights and fulfillment of our duties under social safety and protection laws; (vi) 
establishment, exercise or defence of a legal right or claim; (vii) for relevant public 
interest reasons. 
Among the others, we will process your data for the specific purposes as listed 
below. Please take note that this is not a comprehensive list of any single purpose, 
but merely an exemplification aimed to explain the aforementioned purposes. 
4.1 PURPOSES  OF   PROCESSING   RELATED   TO   THE   WORK   RELATIONSHIP   WITH   THE 
COMPANY 
a. 
MAIN PURPOSE. The main purpose of processing your data regards the 
processing of your donation to the Company, made through our website. 
4.2 PURPOSES OF PROCESSING IN ORDER TO RESPECT LEGAL REQUIREMENTS. 
The Company is subject to several legal requirements. If necessary, your data will 
be processed to guarantee the compliance with those requirements. 
a. 
DATA SAFETY. Data safety represents a relevant legal requirement. If 
necessary, we will process also your data in the context of the essential 
measures to evaluate and guarantee data safety. 
b. 
INTERNAL COMPLIANCE MANAGEMENT. Within the context of compliance 
management (intended as the respect of any rule and disposition of law) we 
will evaluate - for example - if you had received appropriate advices when 
you requested the services and if our personnel had behaved properly and 
accordingly to law requirements. 
c. 
LEGAL REQUIREMENTS. The Company is subject  to  several  legal 
requirements. We will process your data as needed to the extent to be 
respectful of such requirements and we could forward your data to the 
competent Authorities, if set forth by communication law requirements. We 
could also process your data in case of law suit, if required. 
13/03/2025

3 
 
 
 
 
 
 
 
5 
STORAGE PERIOD OF 
YOUR DATA 
 
 
 
 
 
 
 
 
 
 
 
 
 
6 
DATA SHARING 
 
 
 
 
 
 
 
 
 
 
7 
DATA SUBJECT RIGHTS 
 
The Company - Pursuant to art. 5, co. 1, lett. e) of Regulation (EU) 679/2016 - will 
store your data only for the period of time needed to processing the data for 
purposes for which they are processed. 
In the event that the data are processed for different purposes, the same data will 
be automatically erased or saved in a format that doesn9t allow to reach a straight 
conclusion on your identity, as soon as the last specific purpose will be complied. 
For a correct data erasing under Regulation (EU) 679/2016, the Company 
implements a storage policy of your data in accordance with what provided for the 
storage requirements of documents for fiscal purposes and with respect to the 
general rules on limitation period of the contractual claims. Therefore, the 
Company will delete data concerning the services provided upon expiry of a ten 
years period as sets forth by the law. 
 
 
 
Your persona data - in general - will remain under the Company, but could be 
communicated, even without your consent, to supervisory bodies, law enforcement 
or judiciary, Ministry of Finance, Revenue Agency, ministerial bodies and 
competent Authorities, Local bodies, regional and provincial tax commissions, at 
their express request that will treat them as independent data controllers for 
institutional purposes and / or by law during investigations and controls. 
Your data may also be communicated to third parties (for example, partners, 
freelancers etc.), as independent data controllers, for the performance of activities 
instrumental to the aforementioned purposes. 
 
 
 
In your quality of data subject, you shall exercise certain rights toward us as 
provided by Regulation EU 679/2016 and other applicable rules concerning data 
protection. 
The present § 7 contains clarification regarding your rights as data subject under 
Regulation EU 679/2016. 
In particular: 
7.1 DATA SUBJECT RIGHTS. 
In your quality of data subject, you have the following right toward the Company: 
a. 
RIGHT OF ACCESS. At any time you can ask to have information about your 
data as stored by us. Such information regards, among the others, the 
categories of data processed, the purposes of processing, the origin of the 
data in case we didn9t obtained such data directly from you, as well as the 
recipients to who we may have transferred your data, if applicable. You 
could obtain a free copy of your data. If you wish to have further copies, the 
Company reserves the right to ask a remuneration for them. 
b. 
RIGHT OF RECTIFICATION. At any time you can ask a rectification of your 
data. As a consequence, the Company will adopt appropriate actions to 
make your data - as stored and processed by us on an ongoing basis - to be 
kept correct, complete, updated and pertinent, on the basis of the most 
updated data granted. 
13/03/2025

4 
 
 
 
c. 
RIGHT TO ERASURE. At any time you can ask the erasure of your data, subject 
to conditions as provided by the law. For example, that could happen as set 
forth in Article 17 of Regulation EU n. 679/2016: 
¯ 
if your data are no longer necessary in relation to the purposes for 
which they were collected or otherwise processed; 
¯ 
if you withdraw consent on which the processing is based and 
there is no other legal ground for the processing; 
¯ 
if you  object  to  the  processing and  there are  no  overriding 
legitimate grounds for the processing; 
¯ 
if your personal data have been unlawfully processed, unless the 
processing is necessary: (i) for the fulfilment of a legal requirement 
that pretends the processing of your data; in particular for what it 
regards the period of storage of the documents as set forth by the 
law; (ii) for the establishment, exercise or the defence of a legal 
right or claim. 
d. RIGHT TO RESTRICTION OF PROCESSING. At any time you can obtain a 
restriction of processing your data if: 
¯ 
you contest the accuracy of the personal data, for a period enabling 
us to verify the accuracy of the personal data; 
¯ 
the processing is unlawful and you oppose to the erasure of the 
personal data and request the restriction of their use instead; 
¯ 
we no longer need the personal data for the purposes of the 
processing, but they are required by you for the establishment, 
exercise or defence of legal claims; 
¯ 
you have objected to processing pending the verification whether 
the legitimate grounds of the controller override those of the data 
subject. 
e. 
RIGHT TO DATA PORTABILITY. At any  time  you  have  the  right  to  receive  a 
copy of your data as they have been prior provided directly to the Company 
on your explicit request, we will transfer your data - if it is technically 
workable - to another controller. 
f. 
RIGHT TO OBJECT. At any time you can object, on grounds relating to your 
particular situation, to the processing of your data pursuant to point e) or f) 
of Article 6, (1) of Regulation EU n. 679/2016, or whenever personal data 
are processed for direct marketing purposes. In such case, we will no longer 
process your data. This last condition is not applicable if we can 
demonstrate that compelling legitimate grounds for the processing which 
override your interests, rights and freedoms or for the establishment, 
exercise or defence of legal claims. 
g. 
RIGHT TO OBTAIN A COPY OF  THE  SAFEGUARDS.  At  any  time  you  can  obtain a 
copy of the appropriate safeguards implemented for what it regards the data 
transfer toward third countries, if applicable. 
h. 
RIGHT TO  WITHDRAW  THE  CONSENT  AT  ANY  TIME.  At  any  time  you  can 
withdraw the consent, if the processing is based on your consent, without 
prejudice to lawfulness of the processing based on the consent granted prior 
to the withdrawal, by sending a request to the address afore indicated (see 
§ 1) or otherwise with technical procedures made available by the Company 
for the relevant processing. 
13/03/2025

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Name: 
 
 
Surname: 
 
 
Tax Code  
 
Address:  _ 
 
City 
State 
 
Telephone:   
 
Email:   
 
 
 
 
Date:   
 
Signature:   
 
 
 
If not signed by the patient, please indicate relationship to the patient (check one, if applicable): 
  
Parent or guardian of minor patient who could not have consented to health care. 
  
Guardian or conservator of an incompetent patient. 
  
Beneficiary or personal representative of deceased patient. 
7.2 TERMS OF FULFILMENT. 
The Company always tries its best to manage the request within a 30 (thirty) days 
period. Nevertheless, such period could be extended for reasons related to the 
specific right of the data subject or to the complexity of his request. 
7.3 RESTRICTION RELATING TO THE COMMUNICATION OF INFORMATION PENDING THE 
FULFILLING OF REQUIREMENTS PERTAINING RIGHTS OF THE DATA SUBJECTS. 
In certain situations, we may not be able to provide you with the information about
your data due to legal provisions. In case we shall be obliged to decline your request of
information, we will explain the reasons for our denial. 
7.4 CLAIMS TO AUTHORITY. 
In case you won9t consider exhaustive the feedback provided by the Company to
your requests and/or notifications, you have the right to notify a claim to the
competent authority with regard to data protection, pursuant to Article 77 of
Regulation EU n. 679/2016. 
13/03/2025

Retrospective Clinical Study on the Effects of the Longevity Diet and Fasting-Mimicking Diet on Body 
Composition, Age-Related Disease Risk Factors, and Aging Markers. 
 
1. Introduction 
Aging is often accompanied by numerous chronic degenerative conditions that compromise the quality of 
life and lead to dependency. However, a favorable interaction between genetic and environmental factors 
could lead to a significantly higher life expectancy, free from conditions that significantly compromise both 
the quality and duration of life, highlighting that increased longevity is not necessarily accompanied by one 
or more pathological conditions. 
It is widely accepted that the quality and quantity of nutrition are essential factors in maintaining health 
and preventing diseases (1-3). Making healthy and conscious dietary choices can indeed alter the course of 
life in terms of both duration and health. 
Numerous epidemiological studies have demonstrated the critical role of a proper dietary regimen in the 
risk of developing age-related diseases (4). Furthermore, recent studies have highlighted how short periods 
of extreme calorie reduction with adequate nutrient intake (5 days, fasting-mimicking diet, FMD) can impact 
metabolism, producing numerous beneficial effects in both healthy individuals and those with chronic 
degenerative conditions such as cancer, cardiovascular diseases, diabetes, and autoimmune diseases (5-7). 
Theories about senescence usually focus on the aging process and not on the ability of organisms to stay 
young. The longevity diet (8) proposed in this study is intended to be effective in achieving the goal of 
extending youth for as long as possible, optimizing protection, regeneration, and rejuvenation while 
minimizing diseases. It is based on five pillars that support the scientific evidence of the program: basic 
research, epidemiology, clinical studies, centenarian studies, and complex systems studies. 
Essentially, the longevity diet incorporates the dietary tradition of healthy centenarians, characterized by 
simplicity and products typical of their region of origin. 
This type of diet generally consists of three isocaloric meals and one snack (for normal-weight individuals) 
or slightly hypocaloric meals (in the case of overweight or obesity). The diet consists of 50-60% of calories 
from complex carbohydrates, 30-35% of calories from mostly unsaturated fats, and 10-11% of calories from 
proteins, primarily from plant sources and fish, and occasionally from eggs, dairy, and meat, especially for 
those over 65. The diet is formulated to provide all necessary nutrients, including vitamins and minerals. 
 
The fasting-mimicking diet is a dietary intervention in which normal food is replaced with a standardized, 
highly hypocaloric vegan diet for a limited period of 5 consecutive days. This diet is designed to stimulate 
the same processes normally activated during fasting while minimizing the side effects of total food 
deprivation. 
This retrospective clinical study aims to investigate the two different approaches: the longevity diet and the 
fasting-mimicking diet, to assess both their feasibility and effectiveness when used individually or in 
combination. 
 
1.1 Background and Hypotheses 
The Italian population has moved away from the traditional Mediterranean diet and now predominantly 
follows a Western-style diet that is hypercaloric, rich in saturated fats, animal protein, and sugars (12). We 
hypothesize that a return to the original Mediterranean diet with modifications based on the scientific 
evidence of recent decades (longevity diet), and/or short periods of fasting-mimicking diet (5 days), can 
improve the overall health conditions of the population (9-11). 
 
2. Objectives and Purpose of the Study 
Primary Objective: To evaluate whether the longevity diet and the fasting-mimicking diet, either individually 
or in combination, can reduce the percentage of body fat in a cohort of subjects stratified by gender, age, 
body mass index, and defined physio-pathological states. 
Secondary Objectives: To assess whether the longevity diet and the fasting-mimicking diet, either 
individually or in combination, can positively influence parameters related to the general health status of 
the analyzed sample, including (i) percentage of lean mass, (ii) phase angle as an indirect indicator of muscle 
function and cellular integrity, (iii) waist and hip circumference, (iv) total cholesterol, HDL, and LDL. 
 
3. Study Design 
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This is a retrospective, observational, single-center study to evaluate the effect of specific nutritional 
interventions (fasting-mimicking diet (FMD) and longevity diet (LD)) on reducing body fat. To achieve this, 
retrospective data from bioimpedance, anthropometric, and clinical analyses of subjects who underwent at 
least one of the two nutritional interventions (individually or in combination) at the Valter Longo 
Foundation in Milan from 2019 to 2022 will be analyzed. In particular, information from subjects who 
underwent at least two nutritional visits with bioimpedance analysis with a gap of at least 3 months 
between them will be considered. The subjects will be characterized based on (i) the type of nutritional 
intervention (FMD only, LD only, or combinations of LD and FMD); (ii) the duration of the nutritional 
intervention (short, medium, or long); (iii) the degree of obesity (normal weight, overweight, and obese); 
and (iv) physio-pathological status. 
 
4. Data Collection and Analysis 
Data will be collected retrospectively from the electronic medical records of the Valter Longo Foundation. 
Bioimpedance analysis, anthropometric measurements, and clinical parameters including lipid profiles will 
be used to assess changes in body composition, body fat percentage, lean mass percentage, phase angle, 
waist circumference, hip circumference, and blood lipid levels. 
 
5. Statistical Analysis 
Statistical analyses will be performed using appropriate statistical software packages. Descriptive statistics 
will be used to summarize baseline characteristics, and paired t-tests or non-parametric equivalents will be 
used to compare pre- and post-intervention measurements. Analysis of variance (ANOVA) will be used to 
compare outcomes across different subgroups based on type of intervention, duration, degree of obesity, 
and physio-pathological status. Correlation analyses may also be performed to explore associations 
between changes in body composition and other variables. A p-value of less than 0.05 will be considered 
statistically significant. 
 
6. Ethical Considerations 
This study will be conducted in accordance with ethical principles and regulations governing clinical 
research. All data will be de-identified and handled in compliance with data protection regulations. 
Informed consent will be obtained from all subjects included in the study. 
 
7. Conclusion 
This retrospective clinical study aims to investigate the effects of the longevity diet and the 
fasting-mimicking diet, either individually or in combination, on body composition, age-related disease risk 
factors, and aging markers. The study will provide valuable insights into the potential benefits of these 
dietary interventions in promoting health and longevity, which could have significant implications for public 
health and wellness. 
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