The Effect of Shoulder Mobilization on Muscle Strength and Proprioception: a Randomized Double-blind Study
Phase: NA
Conditions: Healthy Male and Female Subjects
Sponsor: Acibadem University

Study Protocol and Statistical Analysis Plan 
Official Title: The Effect of Shoulder Mobilization on Muscle Strength and Proprioception: 
A Randomized Double-blind Study 
NCT Number: NCT06910332 
Document Date: March 07, 2025 
This document contains the full study protocol and statistical analysis plan (SAP). 
Study Protocol 
 
Background and Purpose: 
To investigate the acute effects of a single session of shoulder joint mobilization on muscle 
strength and proprioception in healthy individuals using a randomized, double-blind, 
placebo-controlled design. 
 
Study Design: 
Randomized, double-blind, placebo-controlled trial with 48 healthy university students 
(aged 18-25) allocated to: 
- Mobilization group (n=24): passive glenohumeral joint mobilization 
- Sham group (n=24): simulated mobilization without mechanical glide 
 
Primary Outcomes: 
- Isometric shoulder muscle strength (flexion, abduction, internal/external rotation) 
measured by handheld dynamometer 
- Proprioception assessed via laser-assisted joint position reproduction (JPR) test 
 
Data Collection Time Points: 
- Baseline 
- Immediately after intervention 
 
Statistical Analysis Plan: 
- Normality: histograms, Q-Q plots, Shapiro-Wilk test 
- Baseline group comparisons: t-tests or Mann-Whitney U 
- Main analysis: linear mixed-effects models (group, time, group*time), random effect: 
subject 
- Software: Jamovi v2.4 with GAMLj3 
- Significance: p < 0.05 
 
Ethical Considerations: 
- Approved by Acıbadem University and Acıbadem Healthcare Institutions Medical Research 

Ethics Committee (ATADEK) with the approval number 2025-04/185 
- Informed consent obtained from all participants 
- Registered at ClinicalTrials.gov (NCT06910332)