A Phase 1B Dose-escalation and Phase 2a Study of Carotuximab (TRC105) in Combination With Pazopanib in Patients With Advanced Soft Tissue Sarcoma
Phase: PHASE1
Conditions: Advanced Soft Tissue Sarcoma
Sponsor: Tracon Pharmaceuticals Inc.



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PROCEDURES IN CASE OF EMERGENCY 
Table 1: 
Emergency Contact Information 
Role in Study 
Name 
Address and Telephone number 
Medical Monitor 
Charles Theuer, MD, PhD 
8910 University Center Lane, Suite 700 
San Diego, CA 92122 
Office: (858) 550-0780 x233 
Mobile Phone: (858) 344-9400 
Email: ctheuer@traconpharma.com 
Secondary Medical Monitor 
Ronald Shazer, MD 
Mobile Phone: (310) 922-8039 
Email: rshazer@traconpharma.com  
 

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1. 
SYNOPSIS 
Name of Sponsor/Company: TRACON Pharmaceuticals, Inc.  
Name of Investigational Product: TRC105 
Name of Active Ingredient: TRC105 
Title of Study:  
A PHASE 1B DOSE-ESCALATION AND PHASE 2A STUDY OF TRC105 IN COMBINATION 
WITH PAZOPANIB IN PATIENTS WITH ADVANCED SOFT TISSUE SARCOMA 
Study center(s): This study will be performed at approximately 9 US centers (sites to be 
determined). 
Investigators: To be determined 
Studied period (years):  
Date first patient enrolled: Oct 2013 
Estimated date maximum tolerated dose (MTD) obtained: July 2014 
Estimated date last patient enrolled phase 2: December 2016 
Estimated date last patient completed: June 2017 
Phase of development: 
1b/2a 
 
Rationale:  
Pazopanib is an oral inhibitor of multiple receptor tyrosine kinases, including vascular endothelial 
growth factor receptor VEGFR-1, VEGFR-2, and VEGFR-3 at therapeutic plasma concentrations. 
These receptors are implicated in pathologic angiogenesis, tumor growth, and cancer progression.  
Pazopanib is approved for the treatment of advanced soft tissue sarcoma, following progression on 
one prior systemic therapy, based on improved progression free survival. TRC105 is an antibody to 
CD105, an important angiogenic target on vascular endothelial cells that is distinct from VEGFR.  
TRC105 inhibits angiogenesis, tumor growth and metastases in preclinical models and complements 
the activity of bevacizumab and multi-kinase inhibitors that target the VEGFR.  In a phase 1 study of 
advanced solid tumors, TRC105 therapy caused a global reduction in angiogenic biomarkers and 
reduced tumor burden at doses that were well-tolerated.  In a phase 1b study, the combination of 
TRC105 and bevacizumab produced radiographic reductions in tumor volume in bevacizumab-
refractory patients, and was well tolerated. TRC105 potentiates bevacizumab and VEGFR tyrosine 
kinases (VEGFR TKI) in preclinical models.  By targeting a non-VEGF pathway that is upregulated 
following VEGF inhibition, TRC105 has the potential to complement VEGFR TKIs and could 
represent a major advance in cancer therapy.  Together, the use of TRC105 with pazopanib may 
result in more effective angiogenesis inhibition and improved clinical efficacy over that seen with 
pazopanib alone. In the phase 1b portion of the study, TRC105 at its recommended phase 2 dose of 
10 mg/kg given by weekly intravenous infusion was combined safely with pazopanib given at 800 mg 
p.o. once daily.  Interim data from the Phase 1b/2 study presented at ASCO 2015 indicated that two 
patients with cutaneous angiosarcoma achieved complete responses by RECIST.  Additionally, a 
third patient with cutaneous angiosarcoma treated in the phase 2 portion had a 25% reduction in the 
size of metastatic disease. 
 
Objectives:  
Phase 1b: 

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Primary: 
• 
To evaluate safety and tolerability and determine a  recommended phase 2 dose for TRC105 
when added to standard dose pazopanib in patients with advanced soft tissue sarcoma 
Secondary: 
• 
To assess preliminary evidence of antitumor activity when TRC105 is added to pazopanib, by 
assessing response rate and progression-free survival 
• 
To characterize the pharmacokinetic profile of TRC105 when given with pazopanib 
• 
To evaluate the formation of TRC105 anti-product antibodies 
• 
To correlate efficacy with expression of endoglin on sarcoma tissue 
• 
To explore pharmacodynamic effects on circulating angiogenic biomarkers  
Phase 2: 
Primary: 
• 
To estimate the PFS of patients with advanced soft tissue sarcoma by RECIST 1.1  
• 
To estimate the ORR in a cohort of patients with angiosarcoma by RECIST 1.1 
Secondary: 
• 
To estimate the ORR in patients with advanced soft tissue sarcoma by RECIST 1.1, including 
duration of response 
• 
To estimate the PFS in a cohort of patients with angiosarcoma by RECIST 1.1 
• 
To determine the frequency and severity of adverse events as assessed by NCI CTCAE 
(Version 4.0) 
• 
To characterize the pharmacokinetic profile of TRC105 and pazopanib 
• 
To evaluate the formation of TRC105 anti-product antibodies 
• 
To explore the correlation of efficacy variables (e.g., PFS and ORR) with endoglin expression 
on sarcoma 
• 
To explore the effects of TRC105 on circulating angiogenic protein biomarkers 
Methodology:  
Phase 1b: 
This is a multicenter, open-label, nonrandomized, phase 1b, dose-finding study of TRC105 in 
combination with standard dose pazopanib in patients with advanced soft tissue sarcoma.  Escalating 
doses of i.v. TRC105 will be administered weekly or every two weeks beginning with Dose Level 1 
in combination with oral pazopanib given as 800 mg p.o. once daily.  Intermediate TRC105 doses 
(below the MTD established during the trial) may be explored based upon clinical, PK, and/or 
biomarker data. 
 
 

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Dose Level 
Number of 
Evaluable 
Subjects 
Pazopanib mg 
p.o., once daily 
(with dose 
reduction allowed 
based on 
tolerability) 
TRC105 
mg/kg 
IV 
-1 
3-6 
800 
6 (weekly beginning cycle 2 
day 1) a 
1  
(starting dose) 
3-6 
800 
8 (weekly beginning cycle 2 
day 1) a 
2 
3-6 
800 
10 (weekly beginning cycle 2 
day 1) a 
Expanded Cohort 1 
9-12 
(up to 15 total at the 
MTD) 
800 
MTD (weekly beginning 
cycle 2 day 1) a 
3 
3-6 
800 
• 10 mg/kg weekly during 
cycle 1  
• 15 mg/kg every two weeks  
beginning cycle 2 day 1b 
Expanded Cohort 2 
At least 6 patients 
will be treated at the 
MTD  
800 
• 10 mg/kg weekly during 
cycle 1  
• 15 every two weeks 
beginning cycle 2 day 1b 
a In dose levels -1 to Expanded Cohort 1, pazopanib will be dosed alone during cycle 1 and dosing with 
TRC105 will begin on cycle 2 day 1. The first weekly TRC105 dose will be split into two doses whereby 3 
mg/kg is administered on cycle 2 day 1 and the balance is administered on cycle 2 day 4, the full dose is then 
given weekly thereafter starting with cycle 2 day 8. 
b In dose levels 3 and Expanded Cohort 2, pazopanib and TRC105 dosing will begin on cycle 1 day 1. 
Pazopanib dosing will begin on cycle 1 day 1 and once daily thereafter, and TRC105 will be administered 
weekly during cycle 1. The first weekly TRC105 dose will be split into two doses whereby 3 mg/kg is 
administered on cycle 1 day 1 and the balance is administered on cycle 1 day 4. Starting on cycle 2 day 1 and 
beyond, TRC105 will be administered every two weeks on days 1 and 15 once it is determined that the every 
two week dosing MTD has not been exceeded. 
 
In dose levels -1, 1, 2 and Expanded Cohort 1, patients will receive 800 mg of pazopanib daily p.o. in 
cycle 1 for two to four weeks prior to the initial dose of TRC105 on cycle 2 day 1.  Dose reductions 
of pazopanib are allowed during this two to four week period of dosing based on individual patient 
tolerability.  TRC105 dosing will begin at 8 mg/kg (Dose Level 1) on cycle 2 day 1.  A -1 Dose Level 
has also been included (6 mg/kg) and will be enrolled if 8 mg/kg TRC105 dosed with pazopanib 
exceeds the weekly MTD.  For dose levels -1, 1, 2, and Expanded Cohort 1, the DLT evaluation 
period, for purposes of dose expansion, will be the first 28 days of dosing pazopanib and TRC105 
together (e.g., from cycle 2 day 1 through cycle 2 day 28).  Each cycle will be 28 days in duration. 
For dose levels 3, and Expanded Cohort 2, both pazopanib and TRC105 dosing will begin on C1D1 
and the DLT evaluation period will be the first 28 days of dosing pazopanib with TRC105 every 2 

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weeks (i.e., from cycle 2 day 1 through cycle 2 day 28).  At least 6 patients will be treated at the 
every two week dosing MTD (or top dose level if a MTD is not determined). 
Three patients will be initially enrolled and treated at each dose level.  If none of these 3 patients 
experiences a dose-limiting toxicity (DLT) during the 28-day DLT evaluation period, dose escalation 
will proceed following review of safety data with site staff including the principal investigators at all 
sites. 
If 1 of 3 patients experiences DLT, the dose level will be expanded to 6 patients.  The maximum 
tolerated dose (MTD) will have been exceeded if ≥ 33% of patients experience DLT at a given dose 
level.  DLT will have occurred when a patient has 1 or more toxicity listed in the table below that is 
at least possibly related to the combination of pazopanib and TRC105 during the 28 day DLT 
evaluation period.  Patients who exit the study for reasons other than DLT or any TRC105 dose delay 
>2 days in cycle 2 prior to completion of the 28-day DLT evaluation period will be replaced to ensure 
an adequate safety assessment in each cohort. Patients who experience DLT who receive less than the 
prescribed dose of TRC105 or pazopanib due to documented toxicity during the DLT evaluation 
period will be considered evaluable for dose escalation purposes.  A given TRC105 dose level may be 
reenrolled at > 50% of the pazopanib dose intensity upon agreement of study investigators.  
 
Toxicity Category 
Drug-Related Toxicity/Grade 
Hematologic 
Grade 4 neutropenia for ≥ 5 days 
Febrile neutropenia: grade 4 neutropenia with 
fever > 38.5 ºC both sustained over a 24 hour 
period. 
Neutropenic infection: grade ≥ 3 neutropenia 
with grade ≥ 3 infection 
Anemia ≥ grade 4  
Grade > 4 thrombocytopenia or grade ≥ 3 
thrombocytopenia and grade ≥ 3 hemorrhage 
Nonhematologic 
Grade 3 or 4 nonhematologic toxicity with the 
following exceptions: 
• 
Nausea, vomiting or diarrhea for < 48 
hoursa 
• 
Asymptomatic electrolyte abnormalities 
that are corrected to grade 1 or better in 
< 72 hoursb 
• 
headache lasting less than 48 hours 
aPatients with related grade 3 or 4 diarrhea, nausea or vomiting for ≥ 48 hours despite optimal medical therapy 
will require a one-level dose-reduction of TRC105. 
bPatients with related grade 3 or 4 electrolyte abnormalities that persist for ≥ 72 hours will require a one-level 
dose reduction of TRC105.  
Phase 2: 
This is a multicenter, non-randomized, phase 2 study of TRC105 in combination with standard dose 
pazopanib in patients who have not received pazopanib previously. Sixty three patients are expected 
to be treated in the phase 2 portion of the study at 10 mg/kg. Patients will receive pazopanib once 

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daily and will receive TRC105 at 3 mg/kg on cycle 1 day 1, 7 mg/kg on cycle 1 day 4, and 10 mg/kg 
on cycle 1 day 8 and weekly thereafter. Each cycle is 28 days in duration. 
An additional phase 2 cohort of up to thirteen patients with angiosarcoma (angiosarcoma cohort 1) 
that have progressed following treatment with prior systemic therapy, will be assessed at a TRC105 
dose of 10 mg/kg weekly.  Patients will initially receive weekly single-agent TRC105 with transition 
to treatment with the combination of TRC105 and pazopanib at progression per RECIST 1.1.  
An additional phase 2 cohort (angiosarcoma cohort 2) of up to thirteen patients with angiosarcoma 
that have progressed following treatment with prior systemic therapy, will be assessed at a TRC105 
dose of 10 mg/kg weekly in combination with 800 mg pazopanib once daily for the first cycle starting 
on cycle 1 day 1 followed by TRC105 15 mg/kg administered every two weeks starting from cycle 2 
day 1 onward in combination with pazopanib 800 mg once daily.   
Active patients that are past cycle 1, may be switched to an every 2 week dosing schedule at 
a TRC105 dose of 15 mg/kg every two weeks once  SPONSOR SENDS NOTIFICATION TO 
PROCEED WITH EVERY TWO WEEK DOSING.   
Every two week Administration: 
• 
TRC105 will be administered weekly at 10 mg/kg during cycle 1.  
o The first weekly TRC105 dose (10 mg/kg) will be split into two doses whereby 3 
mg/kg is administered on cycle 1 day 1 and the balance is administered on cycle 1 
day 4.  
• 
Starting on cycle 2 day 1 and beyond, TRC105 may be administered every two weeks on 
days 1 and 15 at the every two week dosing MTD.  
 
Dose reductions of pazopanib are allowed based on individual patient tolerability. 
Each cycle in this cohort is 28 days in duration.  
Number of patients (planned):  
Up to 30 patients with advanced soft tissue sarcoma will be enrolled in the phase 1b portion and 63 
patients will be enrolled in the phase 2 portion, and two additional cohorts of up to 13 patients each 
with angiosarcoma will be enrolled. 
Diagnosis and main criteria for inclusion:  
For the phase 1b dose levels -1, 1, 2, and Expanded Cohort 1, prior to administration of TRC105 on 
cycle 2 day 1, all study patients must maintain eligibility criteria indicating adequate organ function, 
medical status and ECOG performance status (i.e., all patients must maintain inclusion criterion #4, 5 
and 6 and exclusion criteria #8-21 to continue into cycle 2). 
Inclusion Criteria: 
1. Histologically confirmed unresectable soft tissue sarcoma (i.e., non-GIST, non-adipocytic) 
that has progressed following treatment with chemotherapy.  Prior pazopanib is allowed if the 
drug was not discontinued for toxicity ( Phase 1b only) 
2. Histologically confirmed metastatic soft tissue sarcoma (i.e., non-GIST, non-adipocytic) that 
has progressed by RECIST following treatment with anthracycline chemotherapy.  Patients 

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may have received up to four lines of systemic therapy for metastatic disease and no more 
than two lines of combination treatment ( Phase 2 only) 
3. Histologically confirmed locally advanced (e.g. unresectable) or metastatic angiosarcoma that 
has progressed following treatment with prior systemic therapy. Progression must be 
documented on or following the most recent systemic therapy.  Prior pazopanib is allowed if 
the drug was not discontinued for toxicity (Phase 2 angiosarcoma cohorts only) 
4. Measurable disease by RECIST  
5. Age of 12 years or older (patient must weigh ≥ 40 kg) 
6. ECOG performance status ≤ 1 
7. Resolution of all acute adverse events resulting from prior cancer therapies to NCI CTCAE 
grade ≤ 1 or baseline (except alopecia or neuropathy)   
8. Adequate organ function as defined by the following criteria: 
• 
Serum aspartate transaminase (AST; serum glutamic oxaloacetic transaminase [SGOT]) 
and serum alanine transaminase (ALT; serum glutamic pyruvic transaminase [SGPT]) ≤ 
2.5 x upper limit of normal (ULN) or ≤ 5 x ULN in cases of liver metastases  
• 
Total serum bilirubin ≤ 1.5 times the upper limit of normal 
• 
Absolute neutrophil count (ANC) ≥ 1500/μL  
• 
Platelets ≥ 100,000/μL without transfusion support within the past 28 days 
• 
Hemoglobin ≥ 9.0 g/dL without transfusion support within the past 14 days 
(erythropoietin or darbepoietin permitted) 
• 
Serum creatinine ≤ 1.5 times the upper limit of normal or creatinine clearance > 30 
mL/min by Cockcroft-Gault formula 
• 
 INR from 0.8 to 1.2 
9. Willingness and ability to consent for self to participate in study   
10. Willingness and ability to comply with scheduled visits, treatment plan, laboratory tests, and 
other study procedures   
11. Available archival tumor specimen of the soft tissue sarcoma that meets inclusion criterion 
#1, #2 or #3 
Exclusion Criteria:   
1. Prior treatment with TRC105 
2. Prior treatment with a VEGFR TKI (including pazopanib) (Phase 2 only) 
3. Current treatment on another therapeutic clinical trial 
4. Receipt of systemic anticancer therapy, including investigational agents, within 28 days of 
starting study treatment.  If anticancer therapy was given within 28 days of starting study 
treatment, patients may be included if 5 times the elimination half-life of the drug has passed. 
5. No major surgical procedure or significant traumatic injury within 6 weeks prior to study 
registration, and must have fully recovered from any such procedure; date of surgery (if 

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applicable) or the anticipated need for a major surgical procedure within the next six months. 
Note: the following are not considered to be major procedures and are permitted up to 7 days 
before therapy initiation: Thoracentesis, paracentesis, port placement, laparoscopy, 
thorascopy, tube thoracostomy, bronchoscopy, endoscopic ultrasonographic procedures,  
mediastinoscopy, skin biopsies, incisional biopsies, imaging-guided biopsy for diagnostic 
purposes, and routine dental procedures 
6. Patients who have received wide field radiotherapy ≤ 28 days (defined as > 50% of volume 
of pelvic bones or equivalent) or limited field radiation for palliation < 14 days prior to cycle 
1 day 1 or those patients who have not recovered adequately from side effects of such therapy 
7. Uncontrolled chronic hypertension defined as systolic > 150 or diastolic > 90 despite optimal 
therapy (initiation or adjustment of BP medication prior to study entry is allowed provided 
that the average of 3 BP readings at a visit prior to enrollment is < 150/90 mm Hg) 
8. Significant ascites or pericardial or pleural effusion 
9. History of brain involvement with cancer, spinal cord compression, or carcinomatous 
meningitis, or new evidence of brain or leptomeningeal disease.  Patients with radiated or 
resected lesions are permitted, provided the lesions are fully treated and inactive, patients are 
asymptomatic, and no steroids have been administered for at least 28 days.  
10. Angina, MI, symptomatic congestive heart failure, cerebrovascular accident, transient 
ischemic attack, arterial embolism, pulmonary embolism, PTCA or CABG within the past 6 
months. Deep venous thrombosis within 6 months, unless the patient is anti-coagulated 
without the use of warfarin for at least 2 weeks. In this situation, low molecular weight 
heparin is preferred. 
11. Active bleeding or pathologic condition that carries a high risk of bleeding (e.g. hereditary 
hemorrhagic telangiectasia).  Patients who have been uneventfully anti-coagulated with low 
molecular weight heparin are eligible. 
12. Thrombolytic use (except to maintain i.v. catheters) within 10 days prior to first day of study 
therapy 
13. Known active viral or nonviral hepatitis or cirrhosis 
14. History of hemorrhage or hemoptysis (> ½ teaspoon bright red blood) within 3 months of 
starting study treatment 
15. History of peptic ulcer within the past 3 months of treatment, unless treated for the condition 
and complete resolution has been documented by esophagogastroduodenoscopy (EGD) 
within 28 days of starting study treatment 
16. History of gastrointestinal perforation or fistula in the past 6 months, or while previously on 
antiangiogenic therapy, unless underlying risk has been resolved (e.g., through surgical 
resection or repair) 
17. Known human immunodeficiency virus (HIV) or acquired immunodeficiency syndrome 
(AIDS) related illness 
18. Receipt of a strong CYP3A4 inducer within 12 days prior to cycle 1 day 1 or a strong 
CYP3A4 inhibitor within 7 days prior to cycle 1 day 1 (Table 14) 
19.  Pregnancy or breastfeeding.  Female patients must be surgically sterile (i.e.: hysterectomy) 
or be postmenopausal, or must agree to use effective contraception during the study and for 3 

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months following last dose of TRC105.  All female patients of reproductive potential must 
have a negative pregnancy test (serum or urine) within 7 days prior to first dose.  Male 
patients must be surgically sterile or must agree to use effective contraception during the 
study and for 3 months following last dose of TRC105.  The definition of effective 
contraception will be based on the judgment of the Principal Investigator or a designated 
associate.   
20. Other severe acute or chronic medical or psychiatric condition or laboratory abnormality that 
may increase the risk associated with study participation or may interfere with the 
interpretation of study results and, in the judgment of the Investigator, would make the 
patient inappropriate for this study 
TRC105 investigational product dose and mode of administration:  
Phase 1b 
Dosing will begin at 8 mg/kg (Dose Level 1); however a -1 Dose Level has also been included (6 
mg/kg) and will be enrolled if 8 mg/kg is found to exceed the MTD.   
For Dose Level -1, 1, 2 and Expanded Cohort 1, following the appropriate premedication regimen, 
the first weekly TRC105 dose (cycle 2 day 1) will be split into two doses whereby 3 mg/kg is 
administered on cycle 2 day 1 and the balance (e.g., 5 mg/kg for Dose Level 1) is administered on 
cycle 2 day 4.  Beginning with cycle 2 day 8 and thereafter, the full (e.g., 8 mg/kg for Dose Level 1) 
TRC105 dose will be administered i.v. weekly during each 28-day cycle.  
For Dose Levels 3, and Expanded Cohort 2, following the appropriate pre-medication regimen, 
TRC105 will be administered weekly at a dose of 10 mg/kg during cycle 1. The first weekly TRC105 
dose will be split into two doses whereby 3 mg/kg is administered on cycle 1 day 1 and the balance is 
administered on cycle 1 day 4. Beginning with cycle 1 day 8 and for the rest of cycle 1, the full (e.g., 
10 mg/kg) TRC105 dose will be administered i.v. weekly.  Starting on cycle 2 day 1 and beyond, 
TRC105 will be administered at a dose of 15 mg/kg (Dose Level 3) every two weeks on days 1 and 
15 of each 28-day cycle.     
Phase 2 
Patients will receive pazopanib once daily and following appropriate premedication regimen will 
receive TRC105 at 3 mg/kg on cycle 1 day 1, 7 mg/kg on cycle 1 day 4, and 10 mg/kg on cycle 1 day 
8 and weekly thereafter. Each cycle is 28 days in duration.TRC105 dose modification is allowed 
beginning with cycle 2.  
Angiosarcoma cohort 1 
Following the appropriate premedication regimen, TRC105 will be administered weekly with the first 
weekly dose of TRC105 split, with 3 mg/kg administered on cycle 1 day 1 and 7 mg/kg administered 
on cycle 1 day 4, and then the full dose of 10 mg/kg given on cycle 1 day 8 and weekly thereafter. 
TRC105 dose modification is allowed beginning with cycle 2.  
Angiosarcoma cohort 2 
Following the appropriate premedication regimen, TRC105 will be administered weekly with the first 
weekly dose of TRC105 split, with 3 mg/kg administered on cycle 1 day 1 and 7 mg/kg administered 
on cycle 1 day 4, and then the full dose of 10 mg/kg given on cycle 1 day 8 and weekly during Cycle 

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1.  Starting on cycle 2 day 1 and beyond, TRC105 will be administered at a dose of 15 mg/kg every 
two weeks on days 1 and 15 of each 28-day cycle.  
 
Active patients that are past cycle 1, may be switched to an every 2 week dosing schedule at a 
TRC105 dose of 15 mg/kg every two weeks once SPONSOR SENDS NOTIFICATION TO 
PROCEED WITH EVERY TWO WEEK DOSING.   
 
Pazopanib dose and administration: 
In phase 1b and phase 2, patients will receive pazopanib 800 mg once daily beginning on cycle 1 day 
1.  
Angiosarcoma cohort 1 patients will receive pazopanib 800 mg once daily starting at the time of 
disease progression on TRC105 single-agent.  
Angiosarcoma cohort 2 patients will receive pazopanib 800 mg once daily starting on cycle 1 day 1.  
 
Dose modification is allowed based on patient tolerability. 
Duration of treatment:  
Patients are eligible for treatment until disease progression, unacceptable toxicity or withdrawal of 
consent, or other reasons.  A patient should be withdrawn from study treatment if, in the opinion of 
the Investigator, it is medically necessary, or if it is the wish of the patient.  In addition, patients will 
be withdrawn from treatment in the case of: 
1. RECIST 1.1-defined disease progression. In cases where RECIST cannot be applied, 
progression should be based on unequivocal evidence of progressive disease sufficient to 
require a change in therapy. In the angiosarcoma cohort 1, disease progression for 
withdrawal refers to disease progression while receiving TRC105 plus pazopanib 
combination therapy.  
2. A need for surgery, radiation, or for other anticancer therapy not specified in the protocol. 
3. Unable to tolerate pazopanib during cycle 1 (phase 1b dose levels -1, 1, 2 and Expanded 
Cohort 1 only). 
4. Lost to follow-up or noncompliant. 
5. Any TRC105 dose delay > 2 days between cycle 2 day 1 and cycle 2 day 28 (phase 1b 
only). 
6. Pregnancy.  Pregnant patients should be followed for the duration of the pregnancy and 
the outcome of the pregnancy should be documented. 
7. Arterial thrombosis of any grade (including cerebrovascular ischemia, cardiac 
ischemia/infarction, or peripheral or visceral arterial ischemia) or grade 3 or 4 venous 
thrombosis (including pulmonary embolism).   
8. Missed study drug treatment for > 8 consecutive weeks (i.e., both TRC105 and pazopanib 
dosing held). However, patients who cannot tolerate pazopanib or TRC105 therapy and 
who demonstrate a response of complete response (CR), partial response (PR) or stable 
disease (SD) with the combination and are thought to benefit from continued single agent 
therapy may continue on study on TRC105 or pazopanib alone.  
Parameters to be assessed: 
Safety: 
 
Safety assessments will include physical exams, performance status, laboratory results (complete 
blood counts and serum chemistry) and 12-lead ECG’s, and additional studies as clinically indicated.  

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A formally chartered Safety Review Team will review safety data.  In addition, recurring 
teleconferences will be held with Investigators at all clinical sites.   
Pharmacokinetics: 
Serum TRC105 concentrations will be measured using validated methods at the time points specified 
in the Schedule of Events. 
Immunogenicity: 
Anti-product antibodies will be measured using validated methods at time points specified in the 
Schedule of Events. 
Exploratory Biomarkers: 
Concentrations of a panel of angiogenic protein biomarkers in plasma will be measured at baseline 
and during treatment to explore TRC105 pharmacodynamics. 
Efficacy: 
RECIST 1.1 will be applied to measurable disease to assess response and progression.  Tumor 
markers will also be evaluated where available.   
Archival Tumor Specimens:  
Endoglin expression on archival tumor specimens will be correlated with efficacy endpoints (e.g., 
PFS and ORR). 
Statistical methods: 
Evaluable Study Population: 
The study population for safety and efficacy includes all patients receiving at least a portion of 1 dose 
of TRC105.  
The number of patients to be enrolled in this study will depend upon the observed safety profile, 
which will determine the number of patients per dose level and the number of dose escalations.  It is 
anticipated that a total of up to 30 patients will be treated in the phase 1b portion and 89 patients will 
be treated in the phase 2 portion.  
Phase 1: 
The probability of escalation to the next higher dose for each underlying true DLT rate is shown in 
the table below.  For example, for a toxicity that occurs in 5% of patients, there is a > 95% probability 
of escalating.  Conversely, for a common toxicity that occurs with a rate of 70%, the probability of 
escalating is < 5%. 
Probability of Escalation to the Next Dose for Each True Underlying DLT Rate at a Dose Level 
True Underlying DLT 
Rate 
5% 
10% 20% 30% 40% 50% 60% 70% 80% 
90% 
Probability of Escalating 
Dose 
0.97 
0.91 
0.71 
0.49 
0.31 
0.17 
0.08 
0.03 
0.01 
0.001 
The probability of failing to observe toxicity in a sample size of 3 or 6 patients given various true 
underlying toxicity rates in shown in the table below.  For example, with 6 patients, the probability of 
failing to observe toxicity occurring at least 40% of the time is < 5%. 

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Probability of Failing to Observe True Underlying DLT Rate at a Dose Level 
True Underlying 
DLT Rate 
5% 
10% 20% 30% 40% 
50% 
60% 
70% 
80% 
90% 
Probability of 
Failing to 
Observe Toxicity, 
N = 3 
0.86 
0.73 
0.51 
0.34 
0.22 
0.13 
0.006 
0.027 
0.008 
0.001 
Probability of 
Failing to 
Observe Toxicity, 
N = 6 
0.74 
0.53 
0.26 
0.12 
0.05 
0.016 0.004 <0.001 <0.001 <0.001 
Phase 2: 
Primary analysis: 
The primary analysis will test the null hypothesis that the median PFS is equal to 4.6 months.  The 
time-to-event distribution for PFS will be estimated using the Kaplan-Meier method and the analysis 
will be based on the two-sided 90% confidence interval for the median (equivalent to the use of a 
one-sided test at the alpha=0.05 level of significance).   
An additional phase 2 cohort of up to 13 patients with angiosarcoma will be enrolled (angiosarcoma 
cohort 1).  Assuming a 15% response rate by RECIST with pazopanib alone, 5 responders with 
TRC105 alone will provide >90% confidence that the true response rate is >15%.  
A second additional phase 2 cohort of up to 13 patients with angiosarcoma will be enrolled 
(angiosarcoma cohort 2).  Assuming a 15% response rate by RECIST with pazopanib alone, 5 
responders with TRC105 in combination with pazopanib will provide >90% confidence that the true 
response rate is >15%.  
 
Sample size justification: 
Assuming a true median PFS of 4.6 months, a 9-month accrual period, a 6-month follow-up period, 
and based on the use of a one-sided test at the alpha=0.05 level of significance, a sample size of 63 
patients will provide 86% power to detect a 50% increase in the median PFS (i.e., a median of 6.9 
months).  For the angiosarcoma cohorts, the two-sided exact 90% binomial with 5 of 13 responders is 
16.57% and 64.52%. 
  
 
 
 
 

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TABLE OF CONTENTS 
1. 
SYNOPSIS ...................................................................................................................3 
2. 
BACKGROUND ........................................................................................................23 
2.1. 
Angiogenesis and Cancer ...........................................................................................23 
2.1.1. 
Angiogenesis and Advanced Soft Tissue Sarcoma ....................................................23 
2.1.2. 
CD105 and Angiogenesis ...........................................................................................24 
2.2. 
TRC105 Background ..................................................................................................26 
2.2.1. 
Studies with TRC105 ..................................................................................................26 
2.2.1.1. 
105ST101 Phase 1 Monotherapy ................................................................................26 
2.2.1.2. 
Phase 1b 105ST102 Study with Bevacizumab ...........................................................30 
2.2.1.3. 
Phase 1b 105RC101 Study with Axitinib ...................................................................32 
2.2.1.4. 
Phase 1b 105SAR101 Study with Pazopanib .............................................................32 
2.3. 
Study Rationale ...........................................................................................................32 
2.4. 
Population to be Studied .............................................................................................33 
2.5. 
Potential Risks and Benefits to Human Patients.........................................................33 
2.5.1. 
Potential Risks ............................................................................................................33 
2.5.2. 
Potential Benefits ........................................................................................................36 
2.6. 
Justification of the Dose, Schedule and Route of Administration of TRC105 ...........36 
2.6.1. 
Phase 1b Justification .................................................................................................36 
2.6.2. 
Phase 2 Justification ...................................................................................................37 
2.7. 
Conduct .......................................................................................................................38 
3. 
TRIAL OBJECTIVES AND PURPOSE ....................................................................39 
3.1. 
Phase 1b Objectives ....................................................................................................39 
3.1.1. 
Purpose .......................................................................................................................39 
3.1.2. 
Primary objectives ......................................................................................................39 
3.1.3. 
Secondary objectives ..................................................................................................39 
3.2. 
Phase 2 Objectives ......................................................................................................40 
3.2.1. 
Purpose .......................................................................................................................40 
3.2.2. 
Primary objectives ......................................................................................................40 
3.2.3. 
Secondary objectives ..................................................................................................40 
4. 
INVESTIGATIONAL PLAN .....................................................................................41 
4.1. 
Overall Study Design and Plan ...................................................................................41 

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4.1.1. 
Overview .....................................................................................................................41 
4.1.1.1. 
Phase 1b Overview .....................................................................................................41 
4.1.1.2. 
Phase 2 Overview .......................................................................................................43 
4.1.2. 
Trial Procedures ..........................................................................................................44 
4.1.2.1. 
Screening ....................................................................................................................44 
4.1.2.2. 
Trial Period .................................................................................................................45 
4.1.3. 
End of Study Assessments ..........................................................................................47 
4.1.4. 
Post Treatment Follow-up ..........................................................................................48 
5. 
SELECTION AND WITHDRAWAL OF PATIENTS ..............................................63 
5.1. 
Patient Inclusion Criteria ............................................................................................63 
5.2. 
Exclusion Criteria .......................................................................................................64 
5.3. 
Patient Withdrawal Criteria ........................................................................................65 
6. 
TREATMENT OF PATIENTS ..................................................................................67 
6.1. 
Description of TRC105 Study Drug ...........................................................................67 
6.1.1. 
Composition of TRC105.............................................................................................67 
6.1.2. 
TRC105 Dose Level ...................................................................................................67 
6.1.2.1. 
Phase 1b ......................................................................................................................67 
6.1.2.2. 
Phase 2 ........................................................................................................................67 
6.1.3. 
TRC105 Packaging and Labeling ...............................................................................68 
6.1.4. 
TRC105 Storage and Shipping ...................................................................................68 
6.1.5. 
TRC105 Preparation ...................................................................................................68 
6.1.6. 
TRC105 Administration .............................................................................................69 
6.1.7. 
TRC105 Dose Modification/Dose Delays ..................................................................71 
6.1.8. 
Management of TRC105 Infusion Reactions .............................................................74 
6.1.9. 
TRC105 Study Drug Accountability ..........................................................................74 
6.1.10. 
TRC105 Study Drug Handling and Disposal .............................................................75 
6.2. 
Description of Pazopanib ............................................................................................75 
6.2.1. 
Composition of Pazopanib ..........................................................................................75 
6.2.2. 
Pazopanib Dose Level ................................................................................................75 
6.2.3. 
Pazopanib Packaging and Labeling ............................................................................75 
6.2.4. 
Pazopanib Storage Handling and Disposal .................................................................75 
6.2.5. 
Pazopanib Dosing .......................................................................................................76 

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6.2.6. 
Pazopanib Dose Modification .....................................................................................76 
6.2.7. 
Pazopanib Drug Accountability ..................................................................................77 
6.3. 
Concomitant Medications ...........................................................................................77 
6.4. 
Treatment Compliance ................................................................................................78 
6.4.1. 
TRC105 Treatment Compliance .................................................................................78 
6.4.2. 
Pazopanib Treatment Compliance ..............................................................................78 
6.5. 
Patient Enrollment ......................................................................................................78 
7. 
ASSESSMENT OF EFFICACY ................................................................................79 
7.1. 
Radiological Tumor Assessment ................................................................................79 
7.1.1. 
Measurability of Tumor Lesions ................................................................................79 
7.1.1.1. 
Recording Tumor Measurements ...............................................................................80 
7.1.2. 
Definitions of Tumor Response ..................................................................................80 
7.1.2.1. 
Target Lesions ............................................................................................................80 
7.1.2.2. 
Non-Target Lesions ....................................................................................................80 
7.1.2.3. 
Determination of Overall Response ............................................................................81 
8. 
ASSESSMENT OF SAFETY .....................................................................................82 
8.1. 
Safety Parameters .......................................................................................................82 
8.1.1. 
Laboratory Safety Assessments ..................................................................................82 
8.1.1.1. 
Hematology, Serum Chemistry, Coagulation, Pregnancy Test ..................................82 
8.1.1.2. 
Urinalysis ....................................................................................................................82 
8.1.1.3. 
Physical Examination .................................................................................................83 
8.1.1.4. 
Vital Signs ..................................................................................................................83 
8.1.1.5. 
Performance Status .....................................................................................................83 
8.1.1.6. 
ECG ............................................................................................................................83 
8.2. 
Adverse Events ...........................................................................................................83 
8.2.1. 
Definition of Adverse Event .......................................................................................83 
8.2.2. 
Serious Adverse Events ..............................................................................................84 
8.2.2.1. 
Hospitalization ............................................................................................................85 
8.3. 
Reporting Adverse Events ..........................................................................................86 
8.3.1. 
Eliciting Adverse Event Information ..........................................................................86 
8.3.2. 
Adverse Event Reporting Period ................................................................................86 
8.3.3. 
Reporting Requirements .............................................................................................86 

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8.3.4. 
Recording Adverse Events in the Case Report Forms ................................................87 
8.3.5. 
Grading of Adverse Event Severity ............................................................................88 
8.3.6. 
Relationship to TRC105 Study Drug/Pazopanib ........................................................88 
8.3.7. 
Expectedness ...............................................................................................................88 
8.3.8. 
Exposure in Utero .......................................................................................................89 
8.3.9. 
Follow-up of Unresolved Adverse Events ..................................................................90 
8.4. 
Safety Monitoring .......................................................................................................90 
9. 
OTHER ASSESSMENTS ..........................................................................................91 
9.1. 
Other Laboratory Assessments ...................................................................................91 
9.1.1. 
Pharmacokinetics ........................................................................................................91 
9.1.1.1. 
TRC105   Peak and Trough Concentration (Phase 1b dose levels -1, 1, 2 and 
expanded cohort 1 and angiosarcoma cohort 2) .........................................................91 
9.1.1.2. 
TRC105 and Pazopanib Trough Concentration (Phase 1b dose levels 3, 
expanded cohort 2 and Phase 2 including angiosarcoma cohort 1) ............................91 
9.1.2. 
TRC105 Immunogenicity ...........................................................................................91 
9.1.3. 
Protein Biomarkers .....................................................................................................91 
9.1.4. 
Archival  Tumor Specimens .......................................................................................92 
10. 
STATISTICS ..............................................................................................................93 
10.1. 
Statistical Design/Sample Size ...................................................................................93 
10.1.1. 
Phase 1b Statistical Design/Sample Size ....................................................................93 
10.1.1.1. 
Definition of Analyzed Study Populations .................................................................93 
10.1.2. 
Phase 2 Statistical Design/Sample Size ......................................................................94 
10.1.2.1. 
Definition of Analyzed Study Populations .................................................................94 
10.2. 
Data Analysis ..............................................................................................................95 
10.2.1. 
Analysis of Primary Objective....................................................................................95 
10.2.1.1. 
Phase 1b Analysis of Primary Objective ....................................................................95 
10.2.1.2. 
Phase 2 Analysis of Primary Objective ......................................................................95 
10.2.2. 
Analysis of Pharmacokinetics .....................................................................................95 
10.2.3. 
Objective Response ....................................................................................................96 
10.2.4. 
Analysis of Protein Biomarkers ..................................................................................96 
10.2.5. 
Analysis of Immunogenicity .......................................................................................96 
10.2.6. 
Analysis of Archival Tumor Tissue ............................................................................96 
11. 
DIRECT ACCESS TO SOURCE DATA/DOCUMENTS.........................................97 

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12. 
QUALITY CONTROL AND QUALITY ASSURANCE .........................................98 
13. 
ETHICS ......................................................................................................................99 
13.1. 
Institutional Review Board (IRB)/Independent Ethics Committee (IEC) ..................99 
13.2. 
Ethical Conduct of the Study ......................................................................................99 
13.3. 
Written Informed Consent ..........................................................................................99 
13.4. 
Patient Compensation .................................................................................................99 
14. 
DATA HANDLING AND RECORDKEEPING .....................................................100 
14.1. 
Inspection of Records ...............................................................................................100 
14.2. 
Retention of Records ................................................................................................100 
15. 
DEFINITION OF END OF TRIAL .........................................................................101 
15.1. 
End of Trial in all Participating Countries ................................................................101 
15.2. 
End of Trial in a Member State ................................................................................101 
15.3. 
TRACON Discontinuation Criteria ..........................................................................101 
16. 
PUBLICATION OF TRIAL RESULTS ..................................................................102 
17. 
FINANCING AND INSURANCE ...........................................................................103 
18. 
INVESTIGATOR PROTOCOL AGREEMENT: 105SAR101 ...............................104 
19. 
REFERENCES .........................................................................................................105 
20. 
APPENDICES ..........................................................................................................110 
20.1. 
Appendix 1:  National Cancer Institute (NCI) Common Terminology 
Criteria for Adverse Events (CTCAE) .....................................................................110 
20.2. 
Appendix 2:  ECOG Performance Status .................................................................111 
 
LIST OF TABLES 
Table 1: 
Emergency Contact Information ...................................................................................2 
Table 2: 
Abbreviations and Specialist Terms ...........................................................................20 
Table 3: 
Dose Limiting Toxicity Definition and Criteria .........................................................43 
Table 4: 
Schedule of Assessments Phase 1b Dose Levels -1, 1, 2 & Expanded Cohort 
1 ..................................................................................................................................50 
Table 5: 
Schedule of Assessments Phase 1b Dose Levels 3and Expanded Cohort 2 ...............53 
Table 6: 
Schedule of Assessments Phase 2 Soft Tissue Sarcoma ............................................56 
Table 7: 
Schedule of Assessments Phase 2 Angiosarcoma Cohorts 1 &2 ...............................59 
Table 8: 
TRC105 10 mg/kg Weekly Ideal Dosing Schema ......................................................70 

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Table 9: 
TRC105 15 mg/kg Every Two Weeks Ideal Dosing Schema (Ph1b Dose 
Level 3) .......................................................................................................................71 
Table 10: Allowable TRC105 Dose Modifications ....................................................................72 
Table 11: TRC105 dosing after Dose Delay (with no split dosing) ...........................................73 
Table 12: TRC105 Dosing after Dose Delay (with split dosing) ................................................73 
Table 13: Management of TRC105 Infusion Reactions .............................................................74 
Table 14: Strong CYP3A4 Inducers and Inhibitorsa ...................................................................78 
Table 15: Response Evaluation Criteria in Solid Tumors ..........................................................81 
Table 16: Adverse Event Grading ...............................................................................................88 
Table 17: Probability of Escalation to the Next Dose for Each True Underlying DLT 
Rate at a Dose Level ...................................................................................................93 
Table 18: Probability of Failing to Observe True Underlying DLT Rate at a Dose 
Level ...........................................................................................................................93 
 
LIST OF FIGURES 
Figure 1: Single-Dose and Multiple-Dose Pharmacokinetic Data from Study 105ST101 ............27 
 
 
 
 
 
 
 

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Table 2: 
Abbreviations and Specialist Terms  
Abbreviation or specialist term 
Explanation 
ADCC 
Antibody-Dependent Cell-mediated Cytotoxicity 
AE 
Adverse Event 
AFP 
Alpha Fetoprotein 
AIDS 
Acquired Immunodeficiency Syndrome 
ALKs 
Activin receptor-Like Kinases 
ALT 
Alanine Aminotransferase 
ANC 
Absolute Neutrophil Count 
APA 
Anti-Product Antibody 
AST 
Aspartate Aminotransferase 
AUC 
Area Under the Curve 
AUClast 
Time of Last Measurable Concentration of Area Under the Curve 
BALB/c mice 
Mouse Strain 
BMP 
Bone Morphogenic Protein 
BUN 
Blood Urea Nitrogen 
CABG 
Coronary Artery Bypass Graft 
CBC 
Complete Blood Count 
CEA 
Carcinoembryonic Antigen 
CHOP 
Cyclophosphamide Hydroxydaunomycin Oncovin® Prednisone 
CL 
Clearance 
Cmax 
Maximum Serum Concentration  
CPA 
Cyclophosphamide 
CR 
Complete Response 
CRF 
Case Report Form 
CT 
Computed Tomography 
CTC 
Common Terminology Criteria 
dL 
Deciliter 
DLT 
Dose Limiting Toxicity 
DVT 
Deep Vein Thrombosis 
ECG 
Electrocardiogram 
ECOG 
Eastern Cooperative Oncology Group 
ECM 
Extracellular Matrix 
EGFR 
Epidermal Growth Factor Receptor 
ELISA 
Enzyme-Linked ImmunoSorbent Assay 
EOS 
End of Study 
FDA 
Food and Drug Administration 
FGF 
Fibroblast Growth Factor 
FU 
Fluorouracil 
g 
Gram 
GOG 
Gynecologic Oncology Group 
GCP 
Good Clinical Practice 
GIST 
Gastrointestinal Stromal Tumor 
HACA 
Human Anti-Chimeric Antibodies 
HAMA 
Human Anti-Murine Antibodies 
Her-2 
Human epidermal growth factor receptor 2 

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HHT-1 
Hereditary Hemorrhagic Telangiectasia Type 1 
HIF-1-α 
Hypoxia-Inducible Factor-1-α 
HIPAA 
Health Insurance Portability and Accountability Act 
HIV 
Human Immunodeficiency Virus 
HRA 
Health Regulatory Authority 
HUVECs 
Human Umbilical Vein Endothelial Cells 
ICH 
International Conference on Harmonization 
ID 
Identification 
IEC 
Independent Ethics Committee 
IgG 
Immunoglobulin G 
IgM 
Immunoglobulin M 
IL 
Interleukin 
INR 
International Normalized Ratio 
IP 
Intraperitoneal 
IRB 
Institutional Review Board 
i.v. 
Intravenous 
Kd 
Avidity Binding Constant 
kg 
Kilogram 
L 
Liter 
LDH 
Lactate Dehydrogenase 
LOQ 
Limit of Quantification 
μL 
Microliter 
Mg 
Milligram 
mL 
Milliliter 
MACA 
Monkey Anti-Chimeric Antibody 
MAMA 
Monkey Anti-Murine Antibody 
MI 
Myocardial Infarction 
mm 
Millimeter 
MRI 
Magnetic Resonance Imaging 
MTD 
Maximum Tolerated Dose 
NCI 
National Cancer Institute 
NCIC 
National Cancer Institute of Canada 
ng 
Nanogram  
NHP 
Nonhuman Primate 
NOAEL 
No Adverse Effect Level 
PBS 
Phosphate-Buffered Saline 
PD 
Progressive Disease 
PDGF 
Platelet Derived Growth Factor 
PDGFR 
Platelet Derived Growth Factor Receptor 
PlGF 
Placental Growth Factor 
pM 
Picomolar 
PR 
Partial Response 
PSA 
Prostate Specific Antigen 
PTCA 
Percutaneous Transluminal Coronary Angioplasty  
PTT 
Partial Thromboplastin Time 
QA 
Quality assurance 
RCC 
Renal cell carcinoma 

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RECIST 
Response Evaluation Criteria in Solid Tumors 
SAE 
Serious Adverse Event 
sCD105 
Soluble CD105/endoglin 
SCID 
Severe Combined Immunodeficient 
SD 
Stable Disease 
SGOT 
Serum Glutamic Oxaloacetic Transaminase 
SGPT 
Serum Glutamic Pyruvic Transaminase 
SN6j 
Murine parent antibody of TRC105 
sVEGFR2  
Soluble VEGF Receptor 2 
TGF-β 
Transforming Growth Factor 
TSH 
Thyroid Stimulating Hormone 
ULN 
Upper Limit of Normal 
US 
United States of America 
VEGF 
Vascular Endothelial Growth Factor 
VEGFR 
Vascular Endothelial Growth Factor Receptor 
VEGFR TKI 
Vascular Endothelial Growth Factor Receptor Tyrosine Kinase Inhibitor 

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2. 
BACKGROUND 
2.1. 
Angiogenesis and Cancer  
Angiogenesis is required for the survival and growth of solid cancers [2, 3].  It is generally 
accepted that solid cancers have two phases, an avascular phase and a vascular phase [3].  During 
the initial avascular phase, tumors exist as small aggregates of malignant cells supported by 
simple diffusion of oxygen and nutrients.  The progressive growth of solid cancers beyond 
clinically occult sizes requires the continuous formation of new blood vessels, a process known 
as tumor angiogenesis.  Tumor growth and metastasis require angiogenesis.  Therefore, 
inhibition of tumor angiogenesis and selective inhibition of the tumor vasculature represent 
potentially effective strategies for the prevention and treatment of solid cancers.   
Therapies that are directed against targets implicated in the development of tumor angiogenesis 
are attractive for many reasons.  First, except for female reproduction and wound healing, 
angiogenesis in adults is generally part of a pathologic process such as tumor growth or 
choroidal neovascularization.  Second, treatments that interrupt tumor angiogenesis should apply 
broadly to all solid cancers.  Third, angiogenic targets are present in the plasma or on endothelial 
cells themselves.  These targets are readily accessible to antibody treatments, in contrast to 
targets expressed within tumors that are more difficult for antibodies to access.  Fourth, 
angiogenic targets on vascular endothelial cells are less prone to genetic mutation than targets 
expressed by genetically unstable cancer cells.  As a result, development of resistance may be 
more predictable for agents that target endothelial cell functions than for those targeting cancer 
cells. 
Indeed, agents that target pathways required for tumor angiogenesis have an important role in the 
therapy of cancer patients.  The monoclonal antibody bevacizumab, which binds to the 
angiogenic cytokine VEGF, significantly prolongs overall survival for patients with advanced 
colorectal cancer or non-small cell lung cancer when added to standard chemotherapy regimens 
[4, 5].  Bevacizumab is also effective therapy for renal cell cancer and malignant glioma [6-8].  
Orally available small molecule VEGF inhibitors include sunitinib, sorafenib, pazopanib, and 
axitinib which have been shown to prolong survival in patients with metastatic renal cell cancer, 
hepatocellular cancer, colorectal cancer, and sarcoma [9-12].    
2.1.1. 
Angiogenesis and Advanced Soft Tissue Sarcoma 
Sarcomas are rare tumors that originate from mesenchymal tissues (e.g., bone, cartilage, fat and 
muscle).  In the United States, the incidence of bone and soft tissue sarcomas is approximately 
13,000 new cases per year, leading to more than 5,000 deaths annually [13].  According to the 
WHO classification of 2002, over 70 different types of sarcoma have been described [14].  
Localized tumors are curable but patients with metastatic disease have a median survival of 
approximately 12 months.  Standard systemic chemotherapy regimens are poorly tolerated and of 
limited usefulness with response rates in the range of 10-40% [15].    
Recently, the VEGFR TKI pazopanib was approved in patients with advanced soft tissue 
sarcoma who have received prior chemotherapy.  Pazopanib improved progression free survival 
in chemotherapy refractory patients compared to placebo (PFS of 4.6 months with pazopanib 
versus 1.6 months with placebo) and was generally well tolerated, with the most common 

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adverse events seen at higher levels than in patients treated with placebo being fatigue, diarrhea, 
nausea, weight decreased and hypertension.  
The short time to progression following pazopanib treatment emphasizes that sarcoma is a 
disease in need of more effective and well tolerated treatment options.  CD105 (endoglin) is a 
membrane receptor expressed by the proliferating endothelium in sarcoma vasculature as well as 
sarcomatous cells that may be a suitable target for the treatment of sarcoma.   
CD105 is a marker of mesenchymal stem cells, the normal cell type from which sarcomas 
originate [16, 17].  CD105-expressing sarcomas are relatively frequent and express CD105 at 
higher density than carcinoma cell lines.  In one report, high surface expression of CD105 by 
whole cell flow cytometry was seen in 7 of 8 sarcoma cell lines and only 4 of 16 carcinoma cell 
lines [18].  Moreover, the level of CD105 expression correlated with proliferative capacity, and 
the addition of neutralizing anti-CD105 antibodies reversed the increase in proliferation. 
CD105 expression in human sarcoma tumor tissue has been reported by several groups.  
Gromova et al found CD105 on 26 of 49 human gastrointestinal stromal tumors (GIST), and 
higher expression correlated with more aggressive tumors and high risk disease [19]. They 
concluded that CD105 deserves consideration as a target for therapy of GIST.  CD105 
expression on Ewing’s sarcoma has also been reported, and high expression correlates with poor 
survival.  Moreover, CD105 knockdown reversed the increased tumor cell plasticity, 
invasiveness, and anchorage independent growth associated with CD105 expression [20].  Other 
CD105-expressing sarcomas identified in the literature include angiosarcoma, osteosarcoma, 
leiomyosarcoma, malignant fibrous histiocytoma (undifferentiated pleomorphic sarcoma), 
Kaposi’s sarcoma, Wilms tumor, and chondrosarcoma [21-25]. 
In addition to endothelial cells and sarcoma cells, CD105 is found on the surface of benign 
stromal cells present in sarcomatous tumors.  Morozov et al identified a CD105-positive 
sarcoma-derived benign mesenchymal stromal cell (SDBMSC) present in primary sarcoma 
cultures [23].  These cells express pericyte markers and cooperate with endothelial cells in 
vascular tube formation assays. In coculture experiments, SDBMSCs as well as normal CD105-
positive human pericytes markedly stimulated the growth of sarcoma cell lines.   
2.1.2. 
CD105 and Angiogenesis 
CD105 (endoglin) is a homodimeric cell membrane glycoprotein that was initially identified as a 
human leukemia-associated antigen [26] and later also found on endothelial cells [27, 28].  
CD105 is a TGF-β coreceptor that is essential for angiogenesis [29, 30] and CD105 is strongly 
expressed on the proliferating vascular endothelium of solid tumors [28, 31].  All of these 
properties make CD105 an attractive target for the antiangiogenic therapy of cancer [32].  
Vascular targeted therapy may more effectively address large established tumors than 
conventional antiangiogenic therapy such as anti-VEGF therapy [33].  In animal models, CD105 
targeted therapy has demonstrated both vascular targeting effects and antiangiogenic effects by 
inducing regression of established tumors as well as by preventing new tumor formation and 
inhibiting expansion of existing tumors [28, 34-37]. Therefore, CD105 offers a novel alternative 
target relative to the VEGF inhibitors currently available for antiangiogenesis therapy.   
CD105 acts to modulate signaling of multiple kinase receptor complexes of the TGF-β 
superfamily, including TGF-β receptors, activin receptor-like kinases (ALKs) and activin 

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receptors [38].  In the absence of CD105, activation of TGF-β receptors results in 
phosphorylation of SMAD proteins that inhibit endothelial cell growth.  However, activation of 
CD105 by TGF-β modulates SMAD protein phosphorylation.  The end result is release of the 
growth inhibitory effects of TGF-β receptor activation on endothelium.  Not surprisingly, 
prevention of CD105 activation by anti-CD105 antibody acts synergistically with TGF-β to 
inhibit endothelial cell growth [39].  Similarly, CD105 acts in concert with the bone 
morphogenic protein (BMP) receptor to phosphorylate SMAD 1 and 5 in response to binding 
BMP 9 and 10, to activate endothelium [40]. 
CD105 expression is required for endothelial cell proliferation, and CD105 is upregulated in the 
setting of hypoxia through the induction of hypoxia-inducible factor-1-α (HIF-1-α) [41, 42].  
CD105 has also been shown to protect hypoxic cells from apoptosis [43].  The expression of 
CD105 by endothelial cells is essential for the development of new vasculature.  Targeted 
inactivation (knockout) of murine CD105 results in defective vascular development.  Mice 
lacking CD105 die in utero from defective vascular development by gestational day 11 [30].   
CD105 is critical for normal human blood vessel development [44].  CD105 haplotype 
insufficiency causes a well-described syndrome known as hereditary hemorrhagic telangiectasia 
type 1 (HHT-1 or Osler-Weber-Rendu Syndrome).  HHT-1 is a rare autosomal dominant genetic 
disorder characterized by localized angiodysplasia involving the nasal, buccal, gastrointestinal 
mucosa and skin microvasculature.  Angiodysplasia also occurs in vessels from internal organs 
including the lungs, liver and brain [45].  The genotype is manifested in utero, but the phenotype 
does not become apparent for many years following birth.  Affected patients commonly present 
with epistaxis in the second decade of life.  The phenotype of this disorder is limited to vascular 
effects, indicating the specific role of CD105 in the vasculature [46].   
CD105 is highly expressed on the proliferating endothelial cells of tumor vessels including lung, 
breast, colorectal, gastric, liver, endometrial, renal cell, head and neck, and ovarian cancers.  In 
adults, CD105 expression is limited to vascular endothelial cells and proerythroblasts, a red 
blood cell precursor [47].   
CD105 expression is a prognostic factor in solid tumor patients.  High microvessel density of 
CD105-positive vessels has been correlated with poor prognosis in clinical studies of breast 
cancer [48, 49], lung cancer [50], prostate cancer [51, 52], colorectal cancer [53, 54], ovarian 
cancer [55, 56], gastric cancer [57], endometrial cancer [58], astrocytic brain tumors [59], 
hepatocellular carcinoma [60], esophageal adenocarcinoma [61], and head and neck cancer [62, 
63].   
Plasma CD105 levels are prognostic in retrospective studies of cancer patients.  In one study, the 
mean plasma CD105 concentration in 76 patients with colorectal cancer was 4-fold higher than 
the mean value in 40 healthy subjects without cancer [53].  In the study, a positive correlation 
was observed between plasma CD105 concentration and stage of disease.   
Importantly, CD105 expression is upregulated in tumor endothelial cells following inhibition of 
the VEGF pathway.  CD105 expression increased more than 2-fold in human pancreatic cancers 
grown in mice treated with an antibody that binds VEGF [64].  As well, treatment of human 
bladder cancers grown in mice with an antibody that blocks activation of the VEGF receptor 
increased CD105 expression within the core tumor vasculature [65].   

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TRC105 is a novel IgG1 that binds CD105 with high avidity.  Recent studies at Duke University 
explored the in vitro effects of dual angiogenesis inhibition using bevacizumab and TRC105 in 
human umbilical vein endothelial cells (HUVEC).  Combination therapy was found to be more 
potent in decreasing HUVEC proliferation, migration, and tubular network formation than 
bevacizumab or TRC105 treatment alone (manuscript in preparation).  Furthermore, TRC105 
induced apoptosis in HUVEC, and promotes SMAD2/3 phosphorylation while inhibiting 
SMAD1/5/8 signaling, thereby inhibiting angiogenesis in response to VEGF and basic FGF [40]. 
Finally, antibody to mouse CD105 potentiates the activity of multitargeted kinase inhibition that 
targets the VEGFR-2, in mouse bearing cancer grafts (manuscript in preparation).   For these 
reasons, CD105 blockade using TRC105 in combination with VEGF inhibition by pazopanib 
may provide greater clinical benefit than would be seen with either drug alone. 
2.2. 
TRC105 Background 
 
TRC105 is a genetically engineered human/murine chimeric monoclonal antibody directed 
against human CD105 [66], a growth proliferation receptor found on the surface of normal and 
proliferating endothelial cells [28, 34, 42].   
The antibody is an IgG1 kappa immunoglobulin containing murine variable region sequences 
and human constant region sequences [66].  TRC105 has an approximate molecular weight of 
148 kDa. TRC105 has a binding avidity for human CD105 of approximately 5 pM. TRC105 is 
formulated as a phosphate-buffered saline (PBS) solution at a concentration of 7 mg/mL.   
SN6j, the murine parent antibody of TRC105, binds to human umbilical vein endothelial cells 
(HUVECs) with nearly identical avidity as TRC105.  SN6j has been shown to bind the tumor 
vasculature of malignant tissues including breast, colon, rectum, kidney and lung cancers and to 
inhibit the growth of tumor xenografts [35].  Reactivity with tumor tissues is restricted to the 
tumor endothelium, as CD105 is not generally expressed on epithelial tumor cells [34].  TRC105 
induces ADCC on proliferating HUVECs at low conentrations and induces apoptosis and growth 
inhibition at higher concentrations.   
2.2.1. 
Studies with TRC105  
Several studies with TRC105 are underway or have been completed.  An open-label, phase 1, 
multicenter study of TRC105 (Study 105ST101) enrolled fifty patients, who were treated until 
disease progression with TRC105 at 0.01-15 mg/kg/q2wk or 10-15 mg/kg/wk.  Studies of 
TRC105 in prostate, bladder, and ovarian cancer,phase 1b study of TRC105 in combination with 
bevacizumab and a phase 1b study of TRC105 in combination with capecitabine in breast cancer 
have also been completed.  Ongoing studies include a phase 1b study of TRC105 in combination 
with sorafenib in liver cancer, a phase 1b/2 study of TRC105 in combination with axitinib in 
renal cell carcinoma, and phase 2 studies of TRC105 monotherapy in liver cancer and in 
combination with bevacizumab in glioblastoma multiforme (2 studies) and renal cell carcinoma.   
2.2.1.1. 
105ST101 Phase 1 Monotherapy 
2.2.1.1.1. 105ST101 Phase 1 Monotherapy Pharmacokinetics 
In Study 105ST101, TRC105 pharmacokinetics were assessed on patients enrolled at doses up to 
15 mg/kg weekly.  Circulating TRC105 was not measurable above the lower limit of quantitation 

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of the assay (78 ng/mL) in patients receiving doses below 0.3 mg/kg.  TRC105 was measurable 
above the target concentration based on preclinical data (200 ng/mL) for 4 hours at 0.3 mg/kg, 1 
day at 1 mg/kg, 5 days at 3 mg/kg, 7 days at 10 mg/kg TRC105 dosed every two weeks.  Serum 
concentrations expected to saturate CD105 binding sites (≥ 200 ng/mL) were achieved 
continuously at 15 mg/kg q2wk and 10 mg/kg weekly, and TRC105 accumulated at 15 mg/kg 
weekly (Figure 1). 
Figure 1: Single-Dose and Multiple-Dose Pharmacokinetic Data from Study 105ST101 
 
 
 
2.2.1.1.2. 105ST101 Phase 1 Monotherapy Immunogenicity 
In Study 105ST101, serum samples for evaluation of TRC105 immunogenicity, including 
HAMA and HACA, were collected pre-dose on day 1 of each 28 day cycle, at the end of study, 
and then at 4 and 12 weeks after the end of study visit. 

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HAMA and HACA data are available from the phase 1 monotherapy TRC105 trial.  Neither 
HAMA nor HACA were detected in patients treated with CHO-produced TRC105, which will be 
used for all future clinical trials, including this study. 
2.2.1.1.3. 105ST101 Phase 1 Monotherapy Safety  
A total of 50 patients were treated on Study 105ST101 with escalating doses of TRC105 at 0.01, 
0.03, 0.1, 0.3, 1, 3, 10 and 15 mg/kg every two weeks and then 10 and 15 mg/kg weekly.  Dose 
escalation proceeded stepwise until the top dose was reached.  The maximum tolerated dose was 
exceeded at 15 mg/kg weekly and the recommended phase 2 dose of TRC105 was therefore 
determined to be 10 mg/kg weekly or 15 mg/kg every 2 weeks.  Three of 4 patients at 15 mg/kg 
weekly developed grade 3 hypoproliferative anemia (without leucopenia or thrombocytopenia) in 
cycle 2, and one of the three progressed to grade 4 in cycle 3.  Anemia was associated with 
accumulation of TRC105 and characterized by a low reticulocyte production index.  Additional 
laboratory and clinical evaluations excluded common causes of anemia including blood loss, 
hemolysis, plasma volume expansion, inadequate erythropoietin, iron deficiency, and vitamin B-
12 or folate deficiency.  The anemia is believed to result from TRC105-mediated suppression of  
proerythroblasts, the only cells in the bone marrow known to express substantial levels of CD105 
[47].  Anemia was reversible and manageable with dose reduction and standard supportive 
measures including erythropoietin and blood transfusion.   
Infusion reactions, anemia, fatigue, epistaxis and headache were the most frequently observed 
adverse events considered related to TRC105.  The majority of treatment-related adverse events 
were grade 1 or 2. 
Infusion reactions, among the most common adverse events, were usually with the initial 
TRC105 dose and included one or more of the following signs or symptoms: rigors, 
bronchospasm, urticaria, hypertension, hypotension, tachycardia or bradycardia.  Infusion 
reactions were initially reported at 1 mg/kg every 2 weeks for patients receiving TRC105 
produced in NS0 cells without premedication.  TRC105 produced in CHO cells was known to 
more potently engage ADCC in vitro than TRC105 produced in NS0 cells.  Because of this, the 
initial dose level for patients receiving CHO-produced TRC105 was de-escalated to 0.3 mg/kg.  
Despite dose de-escalation, the first two patients at 0.3 mg/kg treated with CHO-produced 
TRC105 experienced grade 2 and grade 3 infusion reactions with the first dose in the absence of 
premedication.  The protocol was therefore amended to require a glucocorticoids -based 
premedication regimen and extend the initial infusion duration from 1 to 4 hours.   
The amendment mandating premedication and extended initial infusion duration successfully 
reduced the frequency and severity of infusion reactions and allowed dose escalation to continue. 
One additional patient who received CHO-produced TRC105 at 1 mg/kg developed a grade 3 
infusion reaction with the third dose given over 2 hours.  This patient had experienced a grade 2 
infusion reaction when the dose was administered over 4 hours.  In all three patients with grade 3 
infusion reactions, TRC105 was not detectable in serum at the time of dosing, which allowed de 
novo binding of TRC105 to CD105 expressing endothelium within the vasculature.  Grade 3 
infusion reactions were not observed in patients dosed at 10 or 15 mg/kg who maintained 
TRC105 serum levels known to saturate CD105 binding sites for the full dosing interval.  At 
dose levels where continuous TRC105 serum levels were achieved, glucocorticoids were safely 
discontinued and the infusion duration reduced to 1 hour. 

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Three patients developed grade 1 cutaneous telangiectasia on the trunk early in the course of 
therapy, all at dose levels of 10 or 15 mg/kg weekly that resulted in continuous serum levels of 
TRC105 known to saturate CD105 sites on human endothelium.  Grade 1 or 2 hemorrhage was 
reported, including intermittent postcoital vaginal bleeding (that also occurred prior to TRC105 
treatment), epistaxis, and superficial gingival bleeding.    
Grade 1 or 2 headaches were observed, mainly in patients treated at doses of TRC105 above 3 
mg/kg.  Headaches began the day following infusion and were generally manageable with 
acetaminophen.  However, grade 2 headache in one patient at 15 mg/kg weekly prompted 
discontinuation prior to completion of the dose-limiting toxicity evaluation period.  Fatigue was 
one of the more common adverse events attributable to TRC105 and was more prevalent at doses 
above 3 mg/kg.   
One patient developed dose-limiting toxicity of grade 4 hemorrhage presenting as melena from a 
gastric ulcer within 5 days of the initial TRC105 infusion at 0.1 mg/kg.  He discontinued 
TRC105 treatment, was transfused 2 units of packed red blood cells and the bleeding resolved 
with nonsurgical management by the time of upper endoscopy.  Serious bleeding was not 
observed following protocol amendment to exclude patients with a history of peptic ulcer disease 
(unless healing was documented) and patients on ulcerogenic medications including non-
steroidal anti-inflammatory drugs.  
Classic toxicities associated with VEGF inhibition, including hypertension, proteinuria and 
thrombosis were not prominent.  One patient with recurrent anal cancer treated at 0.1 mg/kg 
developed proteinuria considered possibly related to TRC105, but proteinuria was also noted 
prior to TRC105 dosing.  Transient hypertension (156/112) without QT changes occurred in a 
single patient one day following infusion of 15 mg/kg, and was controlled by a single dose of 
oral antihypertensive medication. There were no arterial or venous thromboembolic events, nor 
gastrointestinal or other perforations in these patients.    
2.2.1.1.4. 105ST101 Phase 1 Monotherapy Efficacy 
In study 105ST101 stable disease ≥ 2 months was observed in 21 of 45 patients (47%) and stable 
disease ≥ 4 months in 6 of 44 patients (14%).  Decreases in CEA, PSA, or CA-125 were noted in 
7 of 21 patients (33%) and a global decrease in key angiogenic biomarkers was observed with 
treatment.  One patient with castrate-refractory prostate cancer remains on TRC105 treatment 
after 6 years at a TRC105 dose of 0.01 mg/kg every 2 weeks.  He has an ongoing complete PSA 
response, with resolution of bone pain and bone scan normalization. One patient with metastatic 
carcinosarcoma, manifested decreased tumor burden on computerized tomographic scanning and 
maintained stable disease for 20 months on therapy. The latter is especially notable when one 
considers that this patient had received three prior treatments -- carboplatin + paclitaxel for 4 
months, anastrozole for 8 months, and ifosfamide for 2 months -- and had manifested tumor 
progression on each.  In effect, TRC105 provided the most favorable clinical outcome and did so 
as a fourth-line therapy.   

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2.2.1.2. 
Phase 1b 105ST102 Study with Bevacizumab 
2.2.1.2.1. 105ST102 Summary of Safety 
Administration of TRC105 at a dose of 3 mg/kg weekly in combination with bevacizumab was 
well tolerated by three patients without the development of dose limiting toxicity (DLT) and 
dose escalation occurred per the protocol to cohort 2 (6 mg/kg TR105 weekly).  However, the 
concurrent administration of 6 mg/kg TRC105 and bevacizumab on day 1 resulted in the 
development of moderate or severe headaches (including two grade 3 headaches) in four of five 
treated patients.  The 6 mg/kg dose of TRC105 was tolerated when the initial TRC105 dose was 
delayed one week following bevacizumab dosing at 10 mg/kg every two weeks.  Tolerability 
was further improved when the initial dose of TRC105 was given over two days during the first 
week of TRC105 dosing, and dose escalation proceeded to the recommended phase 2 dose of 10 
mg/kg TRC105 weekly.  At the recommended phase 2 dose of both drugs (10 mg/kg), TRC105 
serum concentration were present above target concentration continuously and immunogenicity 
was rarely observed. 
A total of 38 patients were dosed on study across six cohorts and four dose levels. Other than 
headaches that were mitigated by adjusting the dosing schedule of TRC105, the combination of 
TRC105 and bevacizumab was well tolerated.  Two patients experienced grade 3 serious adverse 
suspected events as described below. Most adverse events were graded as 1 or 2 and Grade 4 and 
5 suspected adverse events were not observed. Grade 3 suspected adverse reactions included 
anemia (the dose limiting toxicity of TRC105 established as a single agent; 9 patients), headache 
(4 patients; three of which occurred prior to adjusting the schedule of TRC105), fatigue (2 
patients), brain abscess (1 patient), infusion reaction (in a patient dosed at 6 mg/kg), and 
decreased appetite (1 patient). Headache was the most common suspected adverse event and 
occurred in 31 patients (86.1%); three patients (7.9%) experienced migraine headaches (two of 
grade 1 and one of grade 2 severity). Headaches were treated with triptans and NSAIDs. 
Two patients experienced serious adverse suspected events as described below.  One of the grade 
3 headaches (in a patient dosed at 8 mg/kg without splitting the initial TRC105 dose over two 
days) resulted in hospitalization and patient discontinuation.  One patient dosed at 10 mg/kg of 
TRC105 experienced a serious suspected event of grade 3 brain abscess.  Serious adverse events, 
considered unrelated to TRC105 treatment, included: grade 3 pneumonia and subsequent grade 4 
MRSA sepsis that was complicated by a non Q-wave myocardial infarction during a period of 
hemodynamic instability while hospitalized; grade 3 ileus at the time of symptomatic disease 
progression; grade 5 disease progression; grade 3 left foot cellulitis; grade 3 recurrent 
pneumothorax; grade 3 small bowel obstruction; grade 4 urosepsis. 
At least one sign of the triad of epistaxis, gingival bleeding and telangiectasia, reflecting vascular 
ectasia characteristic of the Osler-Weber-Rendu syndrome of endoglin haplotype insufficiency 
(i.e., an autosomal dominant genetic disorder of heterozygous endoglin expression) was observed 
frequently.  One of these signs or symptoms (of grade 1 or 2 severity) was noted in one of three 
patients treated at 3 mg/kg, four of eight patients treated at 6 mg/kg, four of eight patients treated 
at 8 mg/kg and in all nineteen patients treated at 10 mg/kg of TRC105, generally within the first 
month of dosing.  These signs and symptoms are an expected pharmacologic effects of TRC105 
binding to the endoglin receptor (i.e., they are characteristic of the Osler-Weber-Rendu 
syndrome, that is caused by endoglin haploinsufficiency), and were also observed routinely 

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within the first month of dosing of 10 mg/kg weekly in the single agent TRC105 dose escalation 
study.   
Infusion reactions were, as expected, more notable at lower doses, and were rare at the MTD of 
TRC105 of 10 mg/kg, when TRC105 serum concentrations were maintained continuously.  Two 
of nineteen patients (10%) dosed with 10 mg/kg of TRC105 each experienced a single infusion 
reaction of grade 2 severity, both with the initial dose of TRC105, that required a brief 
interruption of the infusion prior to completion of the scheduled dose. 
Clinically significant anemia was not reported in patients dosed with 3 mg/kg or 6 mg/kg of 
TRC105, was reported in three of seven patients (43%; all grade 3) dosed with 8 mg/kg of 
TRC105, and was observed in nine of 19 (47%; three of grade 2 and six of grade 3 severity) of 
patients dosed with 10 mg/kg of TRC105.  Anemia prompted transfusion of packed red blood 
cells in 10 patients and growth factors were used in five patients. 
Other, less frequent, suspected adverse reactions included hypothyroidism, periorbital edema 
(which was generally noted prior to splitting the initial dose of TRC105), gingival pain, nausea, 
oral pain, vomiting, edema, decreased appetite, dyspnea, nasal congestion, rash and flushing. 
Other adverse events characteristic of each individual drug were not increased in frequency or 
severity when the two drugs were administered together.  Of note, the concurrent administration 
of bevacizumab and TRC105 did not potentiate the known toxicities of bevacizumab of 
hypertension, hemorrhage (including tumor-associated hemorrhage, and pulmonary hemorrhage 
or hemoptysis), or proteinuria.  Reversible posterior leukoencephalopathy syndrome (RPLS), 
congestive heart failure, fistulae, gastrointestinal perforation impaired wound healing, and 
arterial thromboembolic events, were not observed. 
Notably, hypertension and proteinuira, known adverse events of bevacizumab, were rarely 
observed when bevacizumab was given with TRC105.  Mild and transient clinically significant 
hypertension or blood pressure increases were observed in five patients (13%; grade 3 in one 
case (prior to dosing with study drugs) and grade 2 in four cases) and mild transient proteinuria 
was observed in two patients (5%; both grade 2). 
2.2.1.2.2. 105ST102 Summary of Efficacy 
The combination of TRC105 and bevacizumab was active in patients with advanced refractory 
cancer who had progressed on prior bevacizumab or other VEGF inhibitor treatment.  Thirty-
three patients had measurable disease (31 patients) or evaluable disease (2 patients) at baseline 
and received at least one follow up scan and were evaluable for the primary efficacy outcome of 
ORR by RECIST 1.1.  Eighteen patients with measureable disease (58%) had a best response of 
stable disease or partial response.  Two patients (6%), both of whom had been treated with 
bevacizumab and chemotherapy prior to study entry and were then treated at the top dose level of 
TRC105 and bevacizumab, had RECIST 1.1- defined partial responses, including one patient 
with colorectal cancer who continues on treatment for more than 24 months.  A total of 14 
patients (45%) had decreases in overall tumor burden, of whom 10 received prior VEGF 
inhibitor treatment (usually bevacizumab with chemotherapy).  Notably, the duration of 
treatment with TRC105 and bevacizumab of six patients (19% of those with measureable 
disease) exceeded the duration of treatment of the most recent treatment regimen containing a 
VEGF inhibitor (i.e., VEGFR TKI or bevacizumab), received prior to study entry.  These six 

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patients had decreases in tumor burden and several were responders by Choi criteria or RECIST.  
Time to progression ranged from 0 to 437+ days.  Reductions in tumor markers ranging from 5% 
to 85% were observed in 15 of 28 (54%) patients with relevant tumor markers.  Three patients 
demonstrated clinical benefit throughout the study (patient 10038102 at cycle 12 day 22, patient 
10018106 at cycle 7 day 22 and patient 10028101 at cycle 17 day 1); two of them continue to 
receive treatment under a continuation protocol (105CON101).  
2.2.1.3. 
Phase 1b 105RC101 Study with Axitinib 
All eighteen planned patients have been treated in the phase 1 portion of a renal cell cancer study 
of TRC105 plus axitinib as of the time of this protocol amendment (3 at 8 mg/kg and 15 at 10 
mg/kg). Patients have experienced expected TRC105 related adverse events of grade 2 infusion 
reaction and grade 1 epistaxis, gingival bleeding, headache, rash, and fatigue; and expected 
axitinib adverse events of grade 1-3 hypertension, grade 1 hand-foot syndrome and grade 1 
proteinuria. Two patients dosed with TRC105 at 8 mg/kg plus axitinib developed grade 2 
creatinine elevations that reversed with hydration.  Adverse events characteristic of each drug 
were not increased in frequency or severity when both drugs were administered concurrently. 
Eight of 14 evaluable VEGFR TKI refractory patients demonstrated decreases in tumor burden 
ranging from 1.2% to 36% (including two partial responses per RECIST in the fourth line 
treatment setting in ongoing patients). 
2.2.1.4. 
Phase 1b 105SAR101 Study with Pazopanib 
A total of 18 patients have been treated in the phase 1b portion of this study of TRC105 in 
combination with pazopanib. Three patients received 8 mg/kg TRC105 weekly, and 15 patients 
received 10 mg/kg TRC105 weekly in combination with standard dose pazopanib (starting at 800 
mg/daily for 28 days of repeating 28 day cycles) without the development of dose limiting 
toxicity. AEs have been predominantly grade 1 or 2 and have been consistent with the known 
adverse event profiles of TRC105 and pazopanib. 
2.3. 
Study Rationale 
Given the novel mechanism of action and safety profile in early phase clinical studies, including 
a completed study with bevacizumab and an ongoing study with axitinib, TRC105 is a logical 
therapy for use with pazopanib.  Pazopanib inhibits angiogenesis through the inhibition of 
VEGFR tyrosine kinases. TRC105 is an antibody to CD105, an important angiogenic target on 
proliferating endothelial cells that is distinct from the VEGFR and upregulated following VGEF 
inhibition.  TRC105 inhibits angiogenesis, tumor growth and metastases in preclinical models 
and complements the activity of bevacizumab and multi-kinase inhibitors that target the VEGFR.    
In a phase 1b study, the combination of TRC105 and bevacizumab produced radiographic 
reductions in tumor volume in bevacizumab refractory patients.  Together, the use of TRC105 
with pazopanib may result in more effective angiogenesis inhibition and improved clinical 
outcome beyond that seen with pazopanib alone in patients with advanced soft tissue sarcoma 
who have progressed following treatment with first line chemotherapy. Angiosarcoma has been 
shown to express CD105 directly on tumor tissue, in addition to the proliferating tumor vascular 
endothelial cells. Thus, this soft tissue sarcoma histologic subtype may have increased 
responsiveness to TRC105 administered as a single-agent.  

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This trial is a phase 1b/2a dose escalation study of pazopanib in combination with TRC105 in 
order to assess the safety and efficacy of CD105 blockade combined with the VEGFR TKI 
pazopanib.  A standard “3+3” dose- escalation design of patients with advanced soft tissue 
sarcoma will be employed in the phase 1b portion, followed by a phase 2 portion to assess 
progression free survival (PFS) and overall response rate (ORR), safety, and correlative studies 
of expression of endoglin on tumor tissue and plasma angiogenic biomarkers.   The purpose of 
the dose escalation portion is to determine the maximum tolerated dose (MTD) of TRC105 when 
given in combination with pazopanib and to determine the dose limiting toxicities.  Patients 
included in the study will have advanced soft tissue sarcoma that has progressed following first 
line chemotherapy. The phase 2 angiosarcoma cohorts will evaluate TRC105 administered as a 
single-agent and in combination with pazopanib. 
2.4. 
Population to be Studied 
Patients with histologically confirmed advanced soft tissue sarcoma will be enrolled in this trial.   
2.5. 
Potential Risks and Benefits to Human Patients 
2.5.1. 
Potential Risks 
TRC105 
Grade 3 anemia has occurred with TRC105 therapy at the recommended phase 2 dose.  All 
patients treated with TRC105 should be monitored closely for anemia and treated appropriately, 
including the possibility of TRC105 dose reductions.  Anemia may be caused by correctable 
mineral or vitamin deficiency. The anemia related to TRC105 is hypoproductive in nature and is 
reversible with interruption of treatment, transfusion, erythropoietin, and other interventions as 
appropriate. 
Gastrointestinal hemorrhage has occurred with TRC105 therapy.  Patients with active ulcer 
disease or risk factors for ulcer disease are excluded from this study.    
Grade 1 and 2 cutaneous telangiectasia related to TRC105 occur early in the course of therapy 
and have been the source of gingival bleeding and epistaxis.  Telangiectasia are also seen in 
patients with hereditary hemorrhagic telangiectasia (HHT), a disease of CD105 haplotype 
insufficiency.  Patients with HHT are at risk of hemorrhage from abnormal blood vessels and this 
could be exacerbated by treatment with TRC105.  Other contraindications to TRC105 therapy 
include a history of significant hemorrhage or tumors located in the central chest or another 
location where bleeding is associated with high morbidity.  All patients treated with TRC105 
should be monitored for signs of hemorrhage and the risks and benefits of drug treatment 
reevaluated in any patient with hemorrhage. 
Premedication including the use of glucocorticoids is required prior to infusion of TRC105 to 
reduce the frequency and severity of infusion reactions.  Infusion reactions following TRC105 
dosing generally occur with the first TRC105 dose and include a grade 4 vasovagal reaction that 
resolved without sequellae.  Signs and symptoms of TRC105 infusion reactions include 
hypertension, hypotension, dyspnea, bronchospasm, chills/rigors, chills, sweats, fever, nausea, 
tachycardia, bradycardia, EKG changes, flushing, urticaria, pruritus, and headache, generally of 
grade 1 and 2 severity. Potential infusion reactions seen with other therapeutic antibodies include 

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angioedema, asthenia, throat irritation, rhinitis, vomiting, joint pain, fatigue and neurologic 
disorders including inflammation of the spine and/or brain.  
Hypersensitivity reactions with infusions are a potential risk for sensitized patients, and TRC105 
should be used with caution in patients with known hypersensitivity to any component of the 
drug product.  Host anti-TRC105 antibodies to the murine or human portions of CHO-produced 
TRC105 are rare.  In general, the risk of immunogenicity to therapeutic chimeric antibodies is 
small (<10%) and the clinical significance of immunogenicity is not well defined.  The current 
trial will collect serial blood samples for anti-product antibody concentrations to further 
characterize the immunogenicity of TRC105 and potential clinical implications. 
Grade 3 cerebrovascular hemorrhage resulting in hemiparesis occurred in one patient with 
hepatocellular cancer who was thrombocytopenic (who entered the study with a platelet count of 
60,000/uL) in a study of TRC105 with sorafenib.  Patients must have a platelet count of > 
100,000/uL to enter this study (see inclusion criteria). A grade 2 transient ischemic attack was 
reported in a study of TRC105 and pazopanib. Transient Grade 3 hepatic encephalopathy 
occurred in one patient with cirrhosis and hepatocellular carcinoma who received TRC105 in 
combination with sorafenib.   Grade 3 pancreatitis was also observed in this study.  Grade 5 
intracranial hemorrhage occurred in one glioblastoma patient with markedly abnormal blood 
clotting parameters in a study of TRC105 with bevacizumab.  A patient with glioblastoma 
developed temporary confusion and slurred speech following treatment with TRC105 and 
bevacizumab that required hospitalization for observation. Another patient with glioblastoma, 
who underwent resection and had a history of an abnormal collection of cerebral spinal fluid, 
developed a grade 2 cerebral spinal fluid leak. A third patient with glioblastoma with a history of 
recurrent meningitis developed recurrent grade 3 bacterial meningitis while treated with 
bevacizumab and TRC105. 
Grade 3 myocardial infarction (non-Q wave infarct associated with hypertension following an 
infusion reaction) was observed in a patient with hepatocellular cancer following treatment with 
TRC105 that resolved without sequellae.  In addition, a Grade 5 myocardial infarction occurred 
in a patient with coronary artery disease who received TRC105 in combination with sorafenib. 
Patients with evidence of active coronary artery disease are excluded from participation in this 
trial (see exclusion criteria).   
Adult respiratory distress syndrome that required temporary intubation occurred in one patient 
who received TRC105 with pazopanib, from which the patient recovered. Of note, interstitial 
lung disease has been added as an adverse drug reaction and warning/precaution to the core 
safety information for pazopanib. Pneumothorax (collapsed lung) has been observed in trials of 
TRC105 administered with VEGF inhibitors in patients with lung metastases. In addition, 
pnemothorax was observed in one patient, also with lung metastases, receiving single-agent 
TRC105. 
Infections have been observed rarely. Grade 3 infected lipoma/cyst was observed in a Phase 2 
study of TRC105 as a single agent in patients with metastatic bladder cancer. Grade 3 orbital 
cellulitis and grade 3 brain abscess were observed in patients treated with TRC105 and 
bevacizumab and considered possibly related to TRC105.  Grade 1 and 2 gingivitis including 
infection and ulceration has also been observed. Overall, infections have been observed in fewer 

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than 5% of patients and have largely been considered unrelated to treatment with TRC105. 
Reversible grade 3 colitis was reported in a patient treated with TRC105 and pazopanib. 
Grade 1-3 headaches have been observed following TRC105 treatment, generally within hours 
following completion of the initial infusion.  Headaches are throbbing in nature, are not 
associated with radiographic abnormalities, and have responded to treatment with non-steroidal 
anti-inflammatory agents and to triptans. Headaches were particularly common when TRC105 
and bevacizumab were initially dosed on the same day and were ameliorated when TRC105 was 
dosed one week following bevacizumab dosing and given over two days during the initial week 
of dosing. 
Nasal congestion and periorbital edema have been observed with TRC105 dosing, particularly 
when dosed in combination with bevacizumab.  The edema has been transient in nature and 
treated with corticosteroids.  
Fatigue of grade 1- 3 severity has been reported following dosing with TRC105. Maculopapular 
rash and skin flushing of grade 1 and grade 2 severity have also been reported.  A patient 
receiving treatment with TRC105 and sorafenib developed self-limited pancreatitis of grade 2 
severity. 
Pazopanib 
The most common adverse reactions in patients with advanced soft tissue sarcoma (≥20%) are 
fatigue, diarrhea, nausea, decreased weight, hypertension, decreased appetite, vomiting, tumor 
pain, hair color changes, musculoskeletal pain, headache, dysgeusia, dyspnea and skin 
hypopigmentation.  
Further details are available in the package insert [67]. 
Computed Tomography (CT) Scans 
Patients will be exposed to a small amount of radiation as a result of the CT scans required in 
this study.  This degree of exposure has not been associated with harmful health effects. In 
addition, the frequency of CT scans performed in this study is similar to the standard of care 
frequency. Patients with a medical contraindication to CT scans or known Iodinated contrast 
allergies may undergo MRI.  There is minimal risk of MRI imaging in patients able to undergo 
this type of exam including very rare reports of gadolinium-induce nephrogenic systemic fibrosis 
in patients with poor renal function. 
Magnetic Resonance Imaging (MRI): 
MRI is a noninvasive imaging test used to diagnose and evaluate medical conditions. MRI does 
not use radiation and there are no known harmful side-effects. However, MRI may cause anxiety 
for people due to the loud banging made by the machine and the confined space of the testing 
area.  People with pacemakers, aneurysm clips, artificial heart valves, ear implants, or metal 
implants or foreign objects in their body are not permitted to have an MRI. 
 

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Bone Scans: 
A bone scan is a test that can find cancer that has spread to the bones. A bone scan can often find 
a problem days to months earlier than a regular X-ray test. During a bone scan, a radioactive 
substance called a tracer is injected into a vein in your arm. The tracer travels through your 
bloodstream and into your bones. Then a special camera takes pictures of the tracer in your 
bones. A bone scan poses no greater risk than do conventional X-ray procedures.  The tracers 
used in a bone scan produce very little radiation exposure — less than half that of a CT scan.  
Venipuncture 
Patients could also experience side effects from venipuncture for tests that will be done as part of 
this study including pain, tenderness or bruising at the site of collection, and rarely infection may 
occur at the spot where the needle is inserted. 
Other Risks 
This study treatment may involve risks to unborn children therefore patients should not become 
pregnant or father a baby while participating in this study.  Patients should not nurse while on 
this study.  Women of childbearing potential must have a negative pregnancy test before taking 
part in this study.  Patients will be asked to practice an effective method of birth control during 
participation in this study and for three months after the last treatment. The long term risk of 
infertility is unknown. Ovarian failure has been observed with other antiangiogenic agents.  
2.5.2. 
Potential Benefits 
TRC105 is an investigational product, and its efficacy has not been established.  It is possible 
that the administration of TRC105 may result in clinical benefit (i.e., tumor response or 
prolonged stable disease). 
2.6. 
Justification of the Dose, Schedule and Route of Administration of 
TRC105 
2.6.1. 
Phase 1b Justification 
The dose and schedule of TRC105 (8 mg/kg weekly up to 10 mg/kg weekly, 15 mg/kg every 2 
weeks) for phase 1b were selected based on safety, pharmacokinetics and early evidence of 
activity in the phase 1 study of TRC105 for patients with solid tumors (Study 105ST101) and in 
the phase 1b study of TRC105 with bevacizumab.  In phase 1, a weekly dose of 10 mg/kg and a 
dose of 15 mg/kg every two weeks were well tolerated and associated with clinical activity.  
Dose reduction was possible for treatment of anemia.  Doses of 8 and 10 mg/kg TRC105 weekly 
were tolerated in combination with bevacizumab, pazopanib (dose levels 1 and 2 of this study) 
and axitinib and doses of TRC105 of 15 mg/kg every 2 weeks were tolerated in combination 
with sorafenib.  Notably, while the initial schedule of TRC105 was adjusted to decrease the 
frequency of headaches observed with the combination of TRC105 and bevacizumab, there was 
no potentiation of life threatening toxicities associated with bevacizumab, nor potentiation of 
common VEGF inhibitor toxicities (e.g., hypertension and proteinuria).   

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Given the limited experience dosing TRC105 with VEGF inhibitors, it is possible that TRC105 
toxicities will potentiate pazopanib toxicities, and vice versa.  Therefore a TRC105 starting dose 
of 8 mg/kg weekly, which is 20% lower than the single-agent TRC105 MTD identified in the 
phase 1 single agent study and phase 1b study with bevacizumab, was selected. In addition a 6 
mg/kg (-1) dose level has also been included and will be enrolled should 8 mg/kg of TRC105 in 
combination with pazopanib exceed the MTD.  As an added precaution, the first dose of TRC105 
will be delayed by 2 to 4 weeks following pazopanib dosing and administered over 2 days.  In 
addition, pazopanib will be introduced at 800 mg and dose reduced based on patient tolerability 
over a two to four week period prior to the introduction of TRC105. This administration schedule 
will limit immediate Cmax effects of the combination of two drugs that could result in toxicity.  
In the phase 1b portion of the study, dose levels 1 and 2, TRC105 at its recommended phase 2 
dose of 10 mg/kg given by weekly intravenous infusion was combined safely with pazopanib 
given at 800 mg p.o. once daily.  
The phase 1b portion of the study will also evaluate TRC105 administered every two weeks at 15 
mg/kg  starting at cycle 2, following initial dosing with 10 mg/kg weekly for the initial 28 day 
cycle.  TRC105 will be given in combination with pazopanib 800 mg daily. In the phase 1 study 
of single-agent TRC105 (105ST101), TRC105 15 mg/kg administered every 2 weeks was well 
tolerated and resulted in continuous serum concentrations known to saturate CD105 binding sites 
on proliferating endothelium.  An alternative dosing schedule of every 2 weeks TRC105 
treatment in combination with pazopanib, if tolerable, may offer patients more convenience 
without sacrificing safety and efficacy. 
2.6.2. 
Phase 2 Justification 
The Phase 2 portion of the trial is designed to assess the activity of TRC105 in combination with 
the approved dose of pazopanib, as compared to the single agent activity established for 
pazopanib in a prior study.  Activity of the combination will be assessed as PFS (primary 
endpoint) and also ORR.  Efficacy endpoints will be retrospectively correlated with expression 
of endoglin on tumor tissue to determine if direct endoglin expression on sarcoma (in addition to 
the tumor vasculature) may be used as a marker to enrich for responsive sarcoma subtypes in 
future studies.  
Given that the two drugs were well tolerated together at their single agent doses, the phase 2 
portion of the study will dose both drugs starting in cycle 1. Dose reductions of pazopanib, based 
on individual patient tolerability, are permitted starting in cycle 1. 
Five patients with cutaneous angiosarcoma were enrolled in the phase 1b/Phase 2 portion of this 
study and all five patients had tumor reductions, including two patients who progressed on 
pazopanib prior to trial enrollment.  Notabaly, two patients have ongoing complete responses (at 
15 and 22 months of treatment) including one patient who progressed previously after pazopanib 
and docetaxel. These data were presented at ASCO 2016.   Given these signs of activity, two 
additional phase 2 cohorts of up to 13 patients, each with histologically confirmed angiosarcoma 
that have progressed following prior systemic treatment, will be enrolled to assess the overall 
response rate in this subtype of soft tissue sarcoma.   

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Patients in angiosarcoma cohort 1 will initially receive weekly single-agent TRC105 with 
transition to treatment with the combination of TRC105 and pazopanib at progression per 
RECIST 1.1.  
Patients in angiosarcoma cohort 2 will receive TRC105 in combination with pazopanib starting 
on cycle 1 day 1.  
 
2.7. 
Conduct 
The 105SAR101 clinical trial will be conducted in compliance with the protocol, GCP, and the 
applicable regulatory requirements. 

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3. 
TRIAL OBJECTIVES AND PURPOSE 
3.1. 
Phase 1b Objectives 
3.1.1. 
Purpose 
The purpose of this study is to evaluate the safety and effectiveness of TRC105 in combination 
with pazopanib. 
3.1.2. 
Primary objectives  
• To evaluate safety and tolerability and determine a recommended phase 2 dose for 
TRC105 when added to standard dose pazopanib in patients with advanced soft tissue 
sarcoma.  
3.1.3. 
Secondary objectives 
• To assess preliminary evidence of improved antitumor activity when TRC105 is added to 
pazopanib, by assessing response rate and progression-free survival 
• To characterize the pharmacokinetic profile of TRC105 when given with pazopanib 
• To evaluate the formation of TRC105 anti-product antibodies  
• To correlate efficacy with expression of endoglin on sarcoma tissue 
• To explore pharmacodynamic effects on circulating angiogenic biomarkers  

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3.2. 
Phase 2 Objectives 
3.2.1. 
Purpose 
The purpose of this study is to evaluate the safety and effectiveness of TRC105 in combination 
with pazopanib. 
3.2.2. 
Primary objectives  
• To estimate the PFS of patients with advanced soft tissue sarcoma by RECIST 1.1 
• To estimate the ORR in a cohort of patients with angiosarcoma by RECIST 1.1 
3.2.3. 
Secondary objectives 
• To estimate the ORR in patients with advanced soft tissue sarcoma by RECIST 1.1, 
including duration of response  
• To estimate the PFS in a cohort of patients with angiosarcoma by RECIST 1.1 
• To determine the frequency and severity of adverse events as assessed by NCI CTCAE 
(Version 4.0) 
• To characterize the pharmacokinetic profile of TRC105 and pazopanib 
• To evaluate the formation of TRC105 anti-product antibodies  
• To explore the correlation of efficacy variables (e.g., PFS and ORR) with endoglin 
expression on sarcoma 
• To explore the effects of TRC105 on circulating angiogenic protein biomarkers 

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4. 
INVESTIGATIONAL PLAN 
4.1. 
Overall Study Design and Plan 
4.1.1. 
Overview 
All patients must sign a consent form prior to undertaking any study-related procedures.  
Prospective patients will be screened to determine if they qualify for the study within 28 days of 
enrollment. Toxicities will be graded according to the NCI CTCAE Version 4.0.   
4.1.1.1. 
Phase 1b Overview 
This is a multicenter, open-label, nonrandomized, phase 1b, dose-finding study of TRC105 in 
combination with standard dose pazopanib in patients with advanced soft tissue sarcoma.  
Escalating doses of i.v. TRC105 will be administered weekly or every two weeks beginning with 
Dose Level 1 in combination with oral pazopanib given as 800 mg p.o. once daily (see TRC105 
Administration section 6.1.6 and Pazopanib Dosing section 6.2.5).  Intermediate TRC105 doses 
(below the MTD established during the trial) may be explored based upon clinical, PK, and/or 
biomarker data.  
Dose Level 
Number of 
Evaluable 
Subjects 
Pazopanib mg p.o., 
once daily (with 
dose reduction 
allowed based on 
tolerability) 
TRC105 
mg/kg 
IV 
-1 
3-6 
800 
6 (weekly beginning cycle 2 
day 1) a 
1  
(Starting Dose) 
3-6 
800 
8 (weekly beginning cycle 2 
day 1) a  
2 
3-6 
800 
10 (weekly beginning cycle 2 
day 1) a  
Expanded Cohort 1 
9-12 
(up to 15 total at 
the MTD) 
800 
MTD (weekly beginning cycle 
2 day 1)a 
3 
3-6 
800 
• 10 mg/kg weekly during 
cycle 1  
• 15 mg/kg every two weeks  
beginning cycle 2 day 1b 
Expanded Cohort 2 
At least 6 patients 
will be treated at 
the MTD  
800 
• 10 mg/kg weekly during 
cycle 1  
• 15 mg/kg every two weeks 
beginning cycle 2 day 1b 
a In dose levels -1 to Expanded Cohort 1, pazopanib will be dosed alone during cycle 1 and dosing with TRC105 
will begin on cycle 2 day 1. The first weekly TRC105 dose (cycle 2 day 1) will be split into two doses whereby 3 

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mg/kg is administered on cycle 2 day 1 and the balance (e.g., 5 mg/kg for Dose Level 1) is administered on cycle 2 
day 4, the full dose is then given weekly thereafter starting with cycle 2 day 8. 
b In dose levels 3 and expanded cohort 2,  pazopanib and TRC105 dosing will begin on cycle 1 day 1. Pazopanib 
dosing will begin on cycle 1 day 1 and once daily thereafter, and TRC105 will be administered weekly during 
cycle 1. The first weekly TRC105 dose will be split into two doses whereby 3 mg/kg is administered on cycle 1 
day 1 and the balance is administered on cycle 1 day 4. Starting on cycle 2 day 1 and beyond, TRC105 will be 
administered every two weeks on days 1 and 15 once it is determined that the every two week dosing MTD has 
not been exceeded.. 
In dose levels -1, 1, 2 and expanded cohort 1, patients will receive 800 mg of pazopanib daily 
p.o. in cycle 1, which will be two to four weeks in duration prior to the initial dose of TRC105 
given on cycle 2 day 1.  Cycle 1 will therefore be as short as 14 days or as long as 28 days, based 
on the time needed to determine a tolerable dose of pazopanib in each patient.  Dose reductions 
of pazopanib are allowed during cycle 1 based on individual patient tolerability (as well as 
following cycle 1). TRC105 dosing will begin at 8 mg/kg (Dose Level 1) on cycle 2 day 1. Prior 
to administration of TRC105 on cycle 2 day 1, all study patients must maintain eligibility criteria 
indicating adequate organ function, medical status and ECOG performance status (i.e., patients 
must maintain inclusion criterion #6, 8, 9 and 10 and exclusion criteria #8-21 to continue into 
cycle 2) and must be able to tolerate pazopanib.  A -1 Dose Level has also been included (6 
mg/kg) and will be enrolled if 8 mg/kg TRC105 dosed with pazopanib exceeds weekly the MTD. 
For dose levels -1, 1 and 2, the DLT evaluation period, for purposes of dose expansion, will be 
the initial 28 days of dosing with TRC105 and pazopanib (from cycle 2 day 1 through cycle 2 
day 28).  Each cycle will be 28 days in duration.   
For dose levels 3, and Expanded Cohort 2, both pazopanib and TRC105 dosing will begin on 
C1D1 and the DLT evaluation will be the first 28 days of dosing pazopanib with TRC105 every 
2 weeks (i.e., from cycle 2 day 1 through cycle 2 day 28).  At least 6 patients will be treated at 
the every two week dosing MTD (or top dose level if a MTD is not determined). 
Three patients will be initially enrolled and treated at each dose level. If none of these 3 patients 
experiences a dose-limiting toxicity (DLT) during the 28-day evaluation period (extending from 
cycle 2 day 1 until cycle 2 day 28), dose escalation will proceed following review of safety data 
with site staff including the principal investigators at all sites.  
If 1 of 3 patients experiences DLT, the dose level will be expanded to 6 patients.  The maximum 
tolerated dose (MTD) will have been exceeded if ≥ 33% of patients experience DLT at a given 
dose level.  DLT will have occurred when a patient has 1 or more toxicity listed in the table 
below that is at least possibly related to the combination of pazopanib and TRC105 during the 28 
day DLT evaluation period (cycle 2).  Patients who exit the study for reasons other than DLT 
prior to completion of the 28-day DLT evaluation period (cycle 2) will be replaced to ensure an 
adequate safety assessment in each cohort. Patients who experience DLT who receive less than 
the prescribed dose of TRC105 or pazopanib due to documented toxicity during the DLT 
evaluation period will be considered evaluable for dose escalation purposes.  A given TRC105 
dose level may be reenrolled at > 50% of the pazopanib dose intensity upon agreement of study 
investigators. 

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Table 3: 
Dose Limiting Toxicity Definition and Criteria 
Toxicity Category 
Drug-Related Toxicity/Grade 
Hematologic 
Grade 4 neutropenia for ≥ 5 days 
Febrile neutropenia: grade 4 neutropenia with fever > 38.5 ºC both 
sustained over a 24 hour period. 
Neutropenic infection: grade ≥ 3 neutropenia with grade ≥ 3 infection 
Anemia ≥ grade 4  
Grade > 4 thrombocytopenia or grade ≥ 3 thrombocytopenia and 
grade ≥ 3 hemorrhage 
Nonhematologic 
Grade 3 or 4 nonhematologic toxicity with the following exceptions: 
• 
Nausea, vomiting or diarrhea for < 48 hoursa 
• 
Asymptomatic electrolyte abnormalities that are corrected to 
grade 1 or better in < 72 hoursb 
• 
headache lasting less than 48 hours 
aPatients with related grade 3 or 4 diarrhea, nausea or vomiting for ≥ 48 hours despite optimal medical 
therapy will require a one-level dose-reduction of TRC105. 
bPatients with related grade 3 or 4 electrolyte abnormalities that persist for ≥ 72 hours will require a one 
level dose-reduction of TRC105. 
 
4.1.1.2. 
Phase 2 Overview 
This is a multicenter, non-randomized, phase 2 study of TRC105 in combination with standard 
dose pazopanib. Sixty three patients will be treated in the phase 2 portion of the study at 10 
mg/kg weekly; the MTD (top dose level studied as a MTD was not determined) of the phase 1b 
portion of the study (dose levels -1, 1, 2 and expanded cohort 1). Patients will receive pazopanib 
once daily and will receive TRC105 at 3 mg/kg on cycle 1 day 1, 7 mg/kg on cycle 1 day 4, and 
10 mg/kg on cycle 1 day 8 and weekly thereafter. Each cycle is 28 days in duration.  
Two additional phase 2 cohorts of up to thirteen patients each with angiosarcoma that have had 
disease progression following treatment with prior systemic therapy will be assessed.  
Angiosarcoma cohort 1 patients will initially receive weekly single-agent TRC105 at a dose of 
10 mg/kg weekly with transition to treatment with the combination of TRC105 and pazopanib at 
progression per RECIST 1.1.   
Angiosarcoma cohort 2 patients will be assessed at a TRC105 dose of 10 mg/kg weekly in 
combination with 800 mg pazopanib once daily for the first cycle starting on cycle 1 day 1 
followed by TRC105 15 mg/kg administered every two weeks starting from cycle 2 day 1 
onward in combination with pazopanib 800 mg once daily.  
Active patients that are past cycle 1, may be switched to an every 2 week dosing schedule at a 
TRC105 dose of 15 mg/kg every two weeks once SPONSOR SENDS NOTIFICATION TO 
PROCEED WITH EVERY TWO WEEK DOSING.   
• TRC105 will be administered at 10 mg/kg weekly during cycle 1.  

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o The first weekly TRC105 dose (10 mg/kg) will be split into two doses whereby 3 
mg/kg is administered on cycle 1 day 1 and the balance is administered on cycle 
1 day 4.  
• Starting on cycle 2 day 1 and beyond, TRC105 may be administered every two weeks on 
days 1 and 15 at the every two week dosing MTD.  
Pazopanib dosing will start at 800 mg once daily. Dose reductions of pazopanib are allowed 
based on individual patient tolerability. Each cycle in this cohort is 28 days in duration. 
4.1.2. 
Trial Procedures 
All on-study procedures are permitted within the time window indicated in the Schedule of 
Assessments (Table 4, Table 5, Table 6 and Table 7).  
4.1.2.1. 
Screening 
The following screening procedures must be performed within 28 days prior to the first day of 
study therapy.  Qualifying hematology (including Fe studies), serum chemistry (including TSH 
testing), coagulation, physical examination, ECG, pregnancy and urinalysis collected within 7 
days of cycle 1 day 1 do not need to be repeated. The following will be performed according to 
the Schedule of Assessments (Table 4, Table 5, Table 6 and Table 7).  
• Patient signature on current Institutional Review Board (IRB) approved informed consent 
form.  Prior to undergoing any study-specific procedure, patients must read and sign the 
current Institutional Review Board (IRB) approved informed consent form.  Patients may 
sign consent prior to the 28 day screening period. 
• Medical history, baseline signs and symptoms, prior cancer therapy, prior cancer surgery, 
prior radiation therapy, drug allergies, primary diagnosis and demographics. 
• Physical examination including examination of all major body systems, ECOG 
performance status, and vital signs. 
• Hematology (including serum iron, ferritin and total iron binding capacity), coagulation 
(INR) and serum chemistry (including thyroid stimulating hormone (TSH)) to be 
performed locally. 
• Serum or urine pregnancy test for all females of childbearing potential to be performed 
locally. 
• Urinalysis to be performed locally.  Microscopic analysis and/or urine protein-creatinine 
ratio (UPCR) should be performed as clinically indicated. 
• CT or MRI scans of chest, abdomen and pelvis in addition to any other applicable sites of 
disease. Brain and bone scans to be performed if metastasis is suspected prior to starting 
the study. Color photography with caliper or ruler measurements should be used to assess 
cutaneous tumors (target lesions). 
• Single tracing 12-Lead ECG (QT, PR and QRS intervals and heart rate will be captured).  
• Assessment of concomitant medications and treatments from 28 days prior to the start of 
study treatment.  

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• Archival Tumor Tissue Specimens: Archival specimens (formalin-fixed, paraffin-
embedded) of the primary cancer specimen and/or metastatic cancer specimen for each 
study participant. It is preferable that the entire paraffin block be submitted, but if this is 
not feasible, then at least 20 unstained slides are requested for immunohistochemical 
analysis (sections of ~ 5 microns are preferred).  See separate laboratory guide for further 
collection and shipment information. 
4.1.2.2. 
Trial Period 
Qualifying hematology (including Fe studies), blood chemistry (including TSH testing), 
coagulation, urinalysis, physical examination, ECG, and pregnancy test do not need to be 
repeated on cycle 1 day 1 if acceptable screening assessments are performed within 7 days prior 
to the start of study therapy. For dose levels -1, 1, 2 and expanded cohort 1 of the phase 1b 
portion of the study, prior to administration of TRC105 on cycle 2 day 1, all study patients must 
maintain eligibility criteria indicating adequate organ function, medical status and ECOG 
performance status (i.e., all patients must maintain inclusion criterion #4, 5 and 6 and exclusion 
criteria #8-21 to continue into cycle 2).  On days of dosing, all assessments should be performed 
prior to dosing with TRC105 unless otherwise indicated in the Schedule of Assessments. Patients 
will receive 2 cycles (approximately 6-8 weeks) of treatment.  Patients who demonstrate a 
response of CR, PR or SD, will be eligible for additional treatment until progression. For patients 
in the angiosarcoma cohort 1, disease progression for withdrawal from study therapy refers to 
disease progression while receiving TRC105 plus pazopanib combination therapy. Patients who 
cannot tolerate pazopanib or TRC105 therapy and who demonstrate a response of complete 
response (CR), partial response (PR) or stable disease (SD) with the combination and are thought 
to benefit from continued single agent therapy may continue on study on TRC105 or pazopanib 
alone.  Each cycle is 28 days in duration, with the exception of cycle 1 for phase 1b dose levels -
1, 1, 2 and expanded cohort 1 which will be 2 to 4 weeks in duration. The following will be 
performed according to the Schedule of Assessments (Table 4, Table 5, Table 6 and Table 7). 
• Physical examination including examination of all major body systems, ECOG 
performance status, weight and vital signs (heart rate, temperature, blood pressure, 
respiratory rate).  
o Assessment of vital signs during TRC105 infusion: Vital signs are to be assessed 
pre-infusion (i.e., within 30 minutes of starting the infusion), every 30 minutes 
during the infusion (+/- 15 minutes), and at the end of the infusion (i.e., within 30 
minutes after completing the infusion).  Vital signs should be monitored more 
frequently and/or beyond the completion of the infusion if medically indicated 
(e.g. if the patient experiences an infusion reaction that has not yet resolved).  
• Hematology, coagulation (INR) and serum chemistry (including TSH) to be performed 
locally. 
• Serum or urine pregnancy test for all females of childbearing potential to be performed 
locally. 
• Single tracing 12-Lead ECG (QT, PR and QRS intervals and heart rate will be captured). 

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• Urinalysis to be performed locally. Microscopic analysis and/or urine protein-creatinine 
ratio (UPCR) should be performed as clinically indicated. 
• Phase 1b dose levels -1, 1, 2 and expanded cohort 1 and angiosarcoma cohort 2: Blood 
sampling for TRC105 pharmacokinetics will include a pre-infusion trough sample and an 
end-of-infusion peak sample to be analyzed by a third party laboratory (see laboratory 
manual for specific instructions regarding collection times, procedures, processing, 
storage and shipment). 
• Phase 1b dose levels 3, 4 and expanded cohort 2: Blood sampling for TRC105 and 
pazopanib pharmacokinetics will include trough sample collections to be analyzed by a 
third party laboratory (see laboratory manual for specific instructions regarding collection 
times, procedures, processing, storage and shipment). 
• Phase 2 including angiosarcoma cohorts: Blood sampling for TRC105 and pazopanib 
pharmacokinetics will include trough sample collections to be analyzed by a third party 
laboratory (see laboratory manual for specific instructions regarding collection times, 
procedures, processing, storage and shipment). 
• Blood sampling for immunogenicity to be analyzed by a third party laboratory (see 
laboratory manual for specific instructions regarding collection, processing, storage and 
shipment). 
• Blood sampling for protein biomarker analysis by a third party laboratory (see laboratory 
manual for specific instructions regarding collection processing, storage and shipment) 
• CT or MRI scans of chest, abdomen and/or pelvis in addition to any other applicable sites 
of disease. Scan of the chest, abdomen, and pelvis to be performed on-study as outlined 
in the assessment table. Known areas of disease should be consistently followed 
throughout the study.  Assessments should be performed whenever disease progression is 
suspected.  Allowable window for tumor imaging studies is +/- 7 days. Brain and bone 
scans to be performed if metastasis is suspected prior to starting the study and during 
study conduct.  Color photography with caliper or ruler measurements should be used to 
assess cutaneous tumors (target lesions). 
− Patients in angiosarcoma cohort 1 who progress on single agent TRC105 by 
RECIST, tumor assessments will occur every 6 weeks from the new baseline (ie 
scans that showed progression on single agent). 
• Administration of TRC105. TRC105 diluted in normal saline will be administered as a 1 
to 4 hour infusion (+/- 15 minutes) following premedication (see Section 6.1.6) according 
to the pertinent schedule of assessments.  
− Phase 1b Dose Levels -1, 1, 2 and Expanded Cohort 1: TRC105 will not be 
given in cycle 1.  The first TRC105 dose will be split into two doses whereby 3 
mg/kg is administered on cycle 2 day 1, and the balance is administered on cycle 2 
day 4.  The entire weekly dose of TRC105 (6, 8 or 10 mg/kg) is then given on cycle 2 
day 8 and weekly thereafter (e.g., if TRC105 is given at 3 mg/kg on cycle 2 day 1 and 
at 5 mg/kg on cycle 2 day 4, then 8 mg/kg is given on cycle 2 day 8).   

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− Upon completion of enrollment of phase 1b dose level 3 and 
notification by the sponsor, active patients that are past cycle 2, may be 
switched to an every 2 week dosing schedule at the every 2 week dosing 
MTD. 
− Phase 1b Dose Levels 3and Expanded Cohort 2: TRC105 dosing will start on 
cycle 1 day 1 and the first dose will be split into two doses whereby 3 mg/kg is 
administered on cycle 1 day 1, and the balance is administered on cycle 1 day 4.  The 
entire weekly dose of TRC105 (10 mg/kg) is then given on cycle 1 day 8, cycle 1 day 
15 and on cycle 1 day 22. Beginning with cycle 2 day 1, TRC105 will be dosed at 
15mg/kg and every two weeks thereafter (days 1 and 15). 
− Phase 2 Soft Tissue Sarcoma and Phase 2 Angiosarcoma Cohorts: TRC105 
dosing will start on cycle 1 day 1 and the first dose will be split into two doses 
whereby 3 mg/kg is administered on cycle 1 day 1, and the balance is administered on 
cycle 1 day 4.  The entire weekly dose of TRC105 (10 mg/kg) is then given on cycle 
1 day 8 and weekly thereafter. 
− Upon completion of enrollment of phase 1b dose level 3 and 
notification by the sponsor, active patients that are past cycle 1, may be 
switched to an every 2 week dosing schedule at the every 2 week dosing 
MTD. 
TRC105 will be administered intravenously utilizing an infusion pump.  TRC105 has 
been demonstrated to be compatible with polyethylene lined, non-DEHP infusion sets 
and polyvinyl chloride, non-DEHP infusion sets.  TRC105 is required to be 
administered with a 0.2 micron downstream filter.   Duration of infusion 
administration may be increased as medically necessary.   
• Pazopanib dosing. The oral dose of pazopanib is 200 to 800 mg once daily without food 
(at least 1 hour before or 2 hours after a meal) beginning on cycle 1 day 1 in the absence 
of toxicity, except in the phase 2 angiosarcoma cohort 1 where pazopanib will be 
dosed starting at the time of disease progression on TRC105 single-agent and once 
daily thereafter in the absence of toxicity. The dose of pazopanib will not exceed 800 
mg and may be reduced based on individual patient tolerability. Do not crush tablets due 
to the potential for increased rate of absorption which may affect systemic exposure. If a 
dose is missed, it should not be taken if it is less than 12 hours until the next dose. 
• Assessment of adverse events. 
• Assessment of concomitant medications and concomitant treatments. 
4.1.3. 
End of Study Assessments  
Assessments other than TRC105 pharmacokinetics, immunogenicity and protein biomarkers only 
need to be completed if they were not completed during the previous 2 weeks on study (during 
the last 8 weeks on study for radiologic tumor assessments).  The following will be performed 
according to the Schedule of Assessments (Table 4, Table 5, Table 6 and Table 7). 

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• Physical examination including examination of all major body systems, ECOG 
performance status, and vital signs. 
• Single tracing 12-Lead ECG (QT, PR and QRS intervals and heart rate will be captured). 
• Hematology, and serum chemistry (including TSH) to be performed locally. 
• Urinalysis to be performed locally.  Microscopic analysis and/or urine protein-creatinine 
ratio (UPCR) should be performed as clinically indicated. 
• Phase 1b dose levels -1, 1, 2 and expanded cohort 1: Blood sampling for TRC105 
pharmacokinetics to be analyzed by a third party laboratory (see laboratory manual for 
specific instructions regarding collection times, procedures, processing, storage and 
shipment). 
• Phase 1b dose levels 3, 4 and expanded cohort 2: Blood sampling for TRC105 and 
pazopanib pharmacokinetics will include trough sample collections to be analyzed by a 
third party laboratory (see laboratory manual for specific instructions regarding collection 
times, procedures, processing, storage and shipment). 
• Phase 2 including angiosarcoma cohorts: Blood sampling for TRC105 and pazopanib 
pharmacokinetics be analyzed by a third party laboratory (see laboratory manual for 
specific instructions regarding collection times, procedures, processing, storage and 
shipment). 
• Blood sampling for immunogenicity to be analyzed by a third party laboratory (see 
laboratory manual for specific instructions regarding collection, processing, storage and 
shipment). 
• Blood sampling for protein biomarker analysis by a third party laboratory (see laboratory 
manual for specific instructions regarding collection processing, storage and shipment) 
• CT or MRI scans of chest, abdomen and pelvis in addition to any other applicable sites of 
disease.  Color photography with caliper or ruler measurements should be used to assess 
cutaneous tumors (target lesions). 
• Assessment of adverse events. 
• Assessment of concomitant medications and concomitant treatments. 
 
4.1.4. 
 Post Treatment Follow-up 
The following will be performed according to the Schedule of Assessments (Table 4, Table 5, 
Table 6 and Table 7).  Samples should be collected and assessments performed even if new anti-
cancer therapy commences during the follow-up period.  
• Assessment of adverse events. The Investigator should continue to report any related or 
possibly related adverse events that occur beyond the adverse event reporting period. 

IND 131053                                      105SAR101 Protocol Amendment #7, July 22, 2016 
5.3.5.2 Clinical Protocol  
 
 
 
Page 49 of 111 
 
• Phase 1b dose levels -1, 1, 2 and expanded cohort 1: Blood sampling for TRC105 
pharmacokinetics to be analyzed by a third party laboratory (see laboratory manual for 
specific instructions regarding collection, processing, storage and shipment). 
• Phase 1b dose levels 3, 4 and expanded cohort 2: Blood sampling for TRC105 and 
pazopanib pharmacokinetics will include trough sample collections to be analyzed by a 
third party laboratory (see laboratory manual for specific instructions regarding collection 
times, procedures, processing, storage and shipment). 
• Phase 2 including angiosarcoma cohorts: Blood sampling for TRC105 and pazopanib 
pharmacokinetics to be analyzed by a third party laboratory (see laboratory manual for 
specific instructions regarding collection times, procedures, processing, storage and 
shipment). 
• Blood sampling for immunogenicity to be analyzed by a third party laboratory (see 
laboratory manual for specific instructions regarding collection, processing, storage and 
shipment). 
• Serum or urine pregnancy test for all females of childbearing potential to be performed 
locally. 
• Assessment of concomitant medications and concomitant treatments.

IND 131053                                      105SAR101 Protocol Amendment #7, July 22, 2016 
5.3.5.2 Clinical Protocol  
 
 
 
Page 50 of 111 
 
Table 4: 
Schedule of Assessments Phase 1b Dose Levels -1, 1, 2 & Expanded Cohort 1 
Protocol Activities 
Screening 
Cycle 1 [23] 
Cycle 2 [23] 
Cycle 3+ [21] [23] 
End 
of 
Stud
y 
[3] 
28 Day 
Follow-up 
[22] 
Day -28 
 
 
Day 1 
 [2] 
Day 8 
(Day 15) 
(Day 22) 
[1] 
Day 1  
[1] 
Day 4 
[1] 
Day 
8 
[1] 
Day 15  
[1] 
Day 
22 
[1] 
Day 1  
[1] 
Day 8  
[1] 
Day 
15 
[1] 
Day 
22 
[1] 
Baseline Documentation 
  
 
 
  
  
  
  
  
  
  
  
  
  
  
Informed Consent [4] 
X 
 
 
  
  
  
  
  
  
  
  
  
  
  
Medical/Oncology History [5] 
X 
 
 
  
  
  
  
  
  
  
  
  
  
  
Baseline Signs and Symptoms [5] 
X 
 
 
 
 
 
 
 
 
 
 
 
 
 
Physical Examination [6] 
X 
X 
 
X 
  
  
  
  
X 
  
  
  
X 
  
Vital Signs [7] 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
  
Laboratory Studies 
  
 
 
  
  
  
  
  
  
  
  
  
  
  
Hematology [8] 
X+Fe 
X+Fe 
 
X+Fe 
Day -7 
  
  
X 
  
X 
  
X 
  
X 
  
Coagulation [8] 
X 
X 
 
X Day -7 
  
  
  
  
  
  
  
  
 
  
Blood Chemistry [8] 
X+TSH 
X+TSH 
 
X+TSH 
Day -7 
  
  
X 
  
X + 
TSH 
  
 
  
X + 
TSH 
  
Pregnancy Test [9] 
Day -7 
 
 
  
  
  
  
  
  
  
  
  
  
 X 
Urinalysis [10] 
X 
X 
 
X Day -7 
  
  
  
  
X 
  
 
  
X 
  
Treatment w/ Study Drug 
  
 
 
  
  
  
  
  
  
  
  
  
  
  
TRC105 Dosing [11] 
  
 
 
X Split  
X Split  
X 
X  
X  
X  
X  
X  
X  
  
  
Pazopanib [12] 
 
Daily 
 
 
 
 
 
 
 
 
 
 
 
 
Tumor Assessments 
  
 
 
  
  
  
  
  
  
  
  
  
  
  
CT or MRI Scans [13] 
X 
 
 
  
  
  
  
X 
  
  
  
Even 
Cycles 
X 
  
Other Clinical Assessments 
  
 
 
  
  
  
  
  
  
  
  
  
  
  
12-Lead ECG [14] 
X 
 
X Day 8 
  
  
  
 X  
  
 
  
  
  
X  
  
Concomitant 
Medications/Treatments [15] 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
Adverse Events [16] 
 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
Special Laboratory Assessments 
  
 
 
  
  
  
  
  
  
  
  
  
  
  
TRC105 PK Pre-Dose [17] 
  
 
 
 
  
  
X 
  
Even 
Cycles 
  
  
  
  
  
TRC105 PK Post-Dose  [17] 
  
 
 
 
  
  
X 
  
 Even 
Cycles 
  
  
  
 X 
 X 
Anti-product antibodies [18] 
 
X 
 
 
 
 
 
 
 
 
 
 
X 
X 
Protein Biomarkers [19] 
 
 
X 
 
X 
 
 
X 
 
Even 
Cycles 
 
 
 
X 
 
Archival Tumor Tissue [20] 
X 
 
 
 
 
 
 
 
 
 
 
 
 
 

IND 131053                                      105SAR101 Protocol Amendment #7, July 22, 2016 
5.3.5.2 Clinical Protocol  
 
 
 
Page 51 of 111 
 
Schedule of Assessments Footnotes Phase 1b Dose Levels -1, 1, 2 & Expanded Cohort 1 
1. Days of Treatment with TRC105: All assessments should be performed prior to the TRC105 infusion unless otherwise indicated.  Each cycle is 28 days in 
duration, with the exception of cycle 1 which is 2-4 weeks in duration.  
2. Cycle 1 day 1: Hematology (including iron studies), blood chemistry (including TSH testing), urinalysis, physical examination, ECG and pregnancy test not 
required if acceptable screening assessment is performed within 7 days prior to the start of treatment on cycle 1 day 1.  
3. End of Study: The end of study visit should generally occur within 7 days (+/- 1 day) of the last dose of TRC105. Assessments other than TRC105 
pharmacokinetics, immunogenicity and protein biomarkers do not need to be repeated if performed within the previous 2 weeks (previous 8 weeks for radiologic 
tumor assessments). Follow-up visits should occur 28 days following the last dose of TRC105 study drug as outlined in the Schedule of Assessments.  
4. Informed Consent: Must be obtained prior to undergoing any study specific procedure and may occur prior to the 28-day screening period. 
5. Medical and Oncologic History, Demographics and Baseline Signs and Symptoms: All information related to prior anticancer treatment should be recorded. 
Significant medical history and baseline signs and symptoms should be captured from the date of informed consent.  
6. Physical Examination: Examination of major body systems and ECOG performance status.  
7.   Vital Signs: Heart rate, temperature, blood pressure, respiratory rate, weight. Assessment of vital signs during TRC105 Infusions: Vital signs are to be assessed 
pre-infusion (i.e., within 30 minutes of starting the infusion) every 30 minutes during the infusion (+/- 15 minutes) and at the end of the infusion (i.e. within 30 
minutes after completing the infusion). Vital signs should be monitored more frequently and/or beyond the completion of the infusion if medically indicated (e.g. 
if the patient experiences an infusion reaction that has not yet resolved).   
8. Hematology, Chemistry & Coagulation:  Testing to be performed locally.  Thyroid stimulating hormone to be tested at screening, day 1 of each cycle and at 
the end of study visit.  Cycle 1 day 1 assessments only need to be performed if screening assessments were performed more than 7 days prior to cycle 1 day 1.  
Cycle 2 day 1 labs should be performed within 7 days of cycle 2 day 1, results should be reviewed prior to dosing with TRC105 on cycle 2 day 1. Iron studies 
(FE) to be performed according to the schedule of assessments and as clinically indicated during the study.  Thyroid stimulating hormone (TSH) to be performed 
according to the schedule of assessments and as clinically indicated during the study.  Lab assessments may be performed within 3 days prior to TRC105 dosing. 
In addition to the assessments scheduled for the clinical trial, patients should undergo assessment as appropriate to ensure safe treatment. See Section 8.1.1.1 for 
specific panel collection requirements.  
9. Pregnancy Test: Testing to be performed locally. All female patients of childbearing potential must have a negative serum or urine pregnancy test within 7 days 
of cycle 1 day 1 and 28 days following the last dose of TRC105. 
10. Urinalysis: To be performed locally. Cycle 1 day 1 urinalysis only needs to be performed if screening urinalysis was performed more than 7 days prior to cycle 1 
day 1.  Cycle 2 day 1 urinalysis should be performed within 7 days of cycle 2 day 1, results should be reviewed prior to dosing with TRC105 on cycle 2 day 1. 
Microscopic analysis and/or urine protein creatinine ratio or 24-hour urine protein collection should be performed as clinically indicated. 
11. TRC105 Administration: Intravenous TRC105 diluted in normal saline will be administered every 7 days.  The first weekly TRC105 dose will be given on cycle 
2 day 1and split into two doses whereby 3 mg/kg is administered on cycle 2 day 1 and the balance is administered on cycle 2 day 4.  The entire weekly dose of 
TRC105 (6, 8 or 10 mg/kg) is then given on cycle 2 day 8 and weekly thereafter. See Section 6.1.6 for specific TRC105 administration guidelines. 
• 
Upon completion of enrollment of phase 1b dose levels 3 and notification by the sponsor, active patients that are past cycle 2, may be 
switched to an every 2 week dosing schedule at the every 2 week dosing MTD. 
12. Pazopanib Dosing: Oral pazopanib will be dosed once daily at 800 mg starting on cycle 1 day 1 in the absence of toxicity on days 1-28 of each 28 day cycle 
according to the pazopanib package insert.  During cycle 1 pazopanib will be given for a total of 2 to 4 weeks, in the absence of TRC105 to determine a tolerable 
dose in each patient.  Dose reductions are allowed based on individual patient tolerability, including during the initial 2 to 4 week period when pazopanib is 
dosed in the absence of TRC105.  See Section 6.2 for specific dosing guidelines.    
13. CT or MRI Tumor Imaging: Images of the chest, abdomen, and pelvis to be performed at screening, and on-study as outlined in the assessment table.  Known 
areas of disease should be consistently followed throughout the study.  Assessments should be performed whenever disease progression is suspected.  Allowable 

IND 131053                                      105SAR101 Protocol Amendment #7, July 22, 2016 
5.3.5.2 Clinical Protocol  
 
 
 
Page 52 of 111 
 
window for tumor imaging studies is +/- 7 days. Brain and bone scans are to be performed if metastases are suspected at screening or during study therapy. 
Beyond cycle 6 scans will be performed every 3 cycles (cycle 9 day 22, cycle 12 day 22 etc.).  Color photography with caliper measurements should be used to 
assess cutaneous tumors (target lesions). 
14. 12-Lead ECG: Single tracing 12-lead ECG will be performed at screening and at the time-points indicated in the Schedule of Assessments (pre-dose). If the 
patient develops an arrhythmia, the ECG should be repeated on day 1 of each subsequent cycle. For a QTc >500 ms, a repeat ECG for confirmation and 
evaluation of the ECG  by a cardiologist is recommended.   Additional ECGs may be performed on study as clinically indicated.  
15. Concomitant Medications and Treatments: Concomitant medications and treatments will be recorded from 28 days prior to the start of study treatment and up 
to 28 days following the last dose of study treatment. Required TRC105 premedications should be recorded on TRC105 premedications CRF. 
16. Adverse Events: Patients must be followed for adverse events from the first day of treatment with pazopanib or TRC105 study drug until at least 28 days after the 
last dose of TRC105 or pazopanib study drug treatment, or until all serious or TRC105 related toxicities have resolved or are determined to be “chronic” or 
“stable”, whichever is later.  "Baseline-signs and symptoms” will be recorded from the date of informed consent on corresponding case report forms. Any serious 
AE that is possibly related to TRC105 occurring from the time of first TRC105 dose or at any point after the reporting period must be promptly reported to 
TRACON.  
17. TRC105 Pharmacokinetics Trough Concentration: A 5 mL blood sample to be collected at the time-points indicated in the Schedule of Assessments, prior to 
starting the TRC105 infusion.  Samples will be stored at approximately -70°C. Samples will be batch shipped as indicated in the laboratory manual to a third-
party laboratory. See separate laboratory guide for further collection and shipment information. Additional PK samples may also be collected at the time of 
unexpected clinical events.  
 
TRC105 Pharmacokinetics Peak Concentrations: A 5 mL blood sample to be collected within 10 minutes of the completion of the TRC105 infusion at the 
time-points indicated in the Schedule of Assessments.  Samples will be stored at approximately -70°C. Samples will be batch shipped as indicated in the 
laboratory manual to a third-party laboratory. See separate laboratory guide for further collection and shipment information. Additional PK samples may also be 
collected at the time of unexpected clinical events.  
18. Anti-product antibodies: 5 mL blood sample will be collected to assess anti-product antibodies at the time-points indicated in the schedule of assessments and 
stored at approximately -70°C to be analysed by a central laboratory. Samples will be batch shipped as indicated in the laboratory manual to a third-party 
laboratory. See separate laboratory guide for further collection and shipment information. Additional anti-product antibody samples may also be collected at the 
time of unexpected clinical events. 
19. Protein Biomarkers: One 10 mL purple top (K3EDTA) tube will be collected at the time-points indicated in the schedule of assessments and stored at 
approximately -70°C to be analysed by a central laboratory. See separate laboratory guide for further collection and shipment information. 
20. Archival Tumor Tissue: Archival specimens (formalin-fixed, paraffin-embedded) are required of the primary cancer and/or metastatic cancer specimen for each 
study participant. It is preferable that the entire paraffin block be submitted, but if this is not feasible, then at least 20 unstained slides are requested for 
immunohistochemical analysis (sections of 5 microns are preferred).  See separate laboratory guide for further collection and shipment information. 
21. Cycle 3+ Treatment: Patients who demonstrate a response of CR, PR or SD will be eligible for additional treatment until progression. 
22. Follow-up:  The follow-up visit should occur 28 days following the last dose of TRC105. The allowable visit window is +/- 7 days.  
23. Allowable window for each visit within the cycle is +/- 2 days unless otherwise stated. 
 
 

IND 131053                                      105SAR101 Protocol Amendment #7, July 22, 2016 
5.3.5.2 Clinical Protocol  
 
 
 
Page 53 of 111 
 
Table 5: 
Schedule of Assessments Phase 1b Dose Levels 3 and Expanded Cohort 2  
Protocol Activities 
Screening 
Cycle 1 
 (10 mg/kg Weekly) [23] 
Cycle 2 
 (15 mg/kg  every 
two weeks) [23] 
Cycle 3+  
(15 mg/kg  every two 
weeks) [21]  [23] 
End 
of 
Study 
[3] 
28 
Day 
Follow
-up 
[22] 
Day -28 
 
 
Day 1 
[1] [2] 
 
 
Day 4 
 [1] 
Day 8 
[1] 
Day 15 
[1] 
Day 22 
[1] 
Day 1  
[1] 
Day 15 
[1] 
Day 1  
[1] 
Day 15 
[1] 
Baseline Documentation 
  
 
 
  
  
  
  
  
  
  
  
  
Informed Consent [4] 
X 
 
 
  
  
  
  
  
  
  
  
  
Medical/Oncology History [5] 
X 
 
 
  
  
  
  
  
  
  
  
  
Baseline Signs and Symptoms [5] 
X 
 
 
 
 
 
 
 
 
 
  
 
Physical Examination [6] 
X 
X 
 
 
  
  
X 
  
X 
  
X 
  
Vital Signs [7] 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
  
Laboratory Studies 
  
 
 
  
  
  
  
  
  
  
 
  
Hematology [8] 
X+Fe 
X 
 
  
X 
  
X  
 X 
X 
X 
 X 
  
Coagulation [8] 
X 
X 
 
  
  
  
X  
  
  
  
X 
  
Blood Chemistry [8] 
X+TSH 
X+TSH 
 
  
 
  
X+TSH  
  
X + TSH 
 
X + TSH 
  
Pregnancy Test [9] 
Day -7 
 
 
  
  
  
X 
  
X 
  
 
 X 
Urinalysis [10] 
X 
X 
 
  
  
  
X  
  
X 
 
X  
  
Treatment w/ Study Drug 
  
 
 
  
  
  
  
  
  
  
 
  
TRC105 Dosing [11] 
  
X Split  
X Split  
X 
X  
X 
X  
X  
X  
X  
  
  
Pazopanib [12] 
 
Daily  
Daily 
Daily 
  
 
Tumor Assessments 
  
 
 
  
  
  
  
  
  
  
 
  
CT or MRI Scans [13] 
X 
 
 
  
  
  
  
X  
Day 22 
  
Day 22 
Even 
Cycles 
 
 X 
  
Other Clinical Assessments 
  
 
 
  
  
  
  
  
  
  
 
  
12-Lead ECG [14] 
X 
 
 
  
 X  
  
  
  
 
  
 X 
  
Concomitant 
Medications/Treatments [15] 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X  
X 
Adverse Events [16] 
 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
Special Laboratory Assessments 
  
 
 
X 
  
  
  
  
  
  
X 
  
Trough PK [17] 
  
X 
 
  
 
  
X 
 X 
X &  
Even 
Cycles 
X (Cycle 
3 only) 
 X 
X 
Anti-product antibodies [18] 
 
X 
 
 
 
 
X 
 
Even 
Cycles 
 
 X 
X 
Protein Biomarkers [19] 
 
 
X 
 
 
 
 
X 
 
Even 
Cycles 
 
X 
 
Archival Tumor Tissue [20] 
X 
 
 
 
 
 
 
 
 
 
 
 

IND 131053                                      105SAR101 Protocol Amendment #7, July 22, 2016 
5.3.5.2 Clinical Protocol  
 
 
 
Page 54 of 111 
 
Schedule of Assessments Footnotes for Phase 1b Dose Levels 3, 4 and Expanded Cohort 2 
1. Days of Treatment with TRC105: All assessments should be performed prior to the TRC105 infusion unless otherwise indicated.  Each cycle is 28 days in 
duration.  
2. Cycle 1 day 1: Hematology (including iron studies), blood chemistry (including TSH testing), urinalysis, physical examination, ECG and pregnancy test not 
required if acceptable screening assessment is performed within 7 days prior to the start of treatment on cycle 1 day 1.  
3. End of Study: The end of study visit should generally occur within 14 days (+/- 1 day) of the last dose of TRC105. Assessments other than 
pharmacokinetics, immunogenicity and protein biomarkers do not need to be repeated if performed within the previous 2 weeks (previous 8 weeks for 
radiologic tumor assessments). Follow-up visits should occur 28 days following the last dose of TRC105 study drug as outlined in the Schedule of 
Assessments.  
4. Informed Consent: Must be obtained prior to undergoing any study specific procedure and may occur prior to the 28-day screening period. 
5. Medical and Oncologic History, Demographics and Baseline Signs and Symptoms: All information related to prior anticancer treatment should be 
recorded. Significant medical history and baseline signs and symptoms should be captured from the date of informed consent.  
6. Physical Examination: Examination of major body systems and ECOG performance status.  
7. Vital Signs: Heart rate, temperature, blood pressure, respiratory rate, weight. Assessment of vital signs during TRC105 Infusions: Vital signs are to be 
assessed pre-infusion (i.e., within 30 minutes of starting the infusion) every 30 minutes during the infusion (+/- 15 minutes) and at the end of the infusion 
(i.e. within 30 minutes after completing the infusion). Vital signs should be monitored more frequently and/or beyond the completion of the infusion if 
medically indicated (e.g. if the patient experiences an infusion reaction that has not yet resolved).   
8. Hematology, Chemistry & Coagulation:  Testing to be performed locally.  Thyroid stimulating hormone to be tested at screening, day 1 of each cycle and 
at the end of study visit.  Cycle 1 day 1 assessments only need to be performed if screening assessments were performed more than 7 days prior to cycle 1 
day 1.  Iron studies (FE) to be performed according to the schedule of assessments and as clinically indicated during the study.  Thyroid stimulating hormone 
(TSH) to be performed according to the schedule of assessments and as clinically indicated during the study.  Lab assessments may be performed within 3 
days prior to TRC105 dosing. In addition to the assessments scheduled for the clinical trial, patients should undergo assessment as appropriate to ensure safe 
treatment. See Section 8.1.1.1 for specific panel collection requirements.  
9. Pregnancy Test: Testing to be performed locally. All female patients of childbearing potential must have a negative serum or urine pregnancy test within 7 
days of cycle 1 day 1, day 1 of every cycle starting with cycle 2, and 28 days following the last dose of TRC105. 
10. Urinalysis: To be performed locally. Cycle 1 day 1 urinalysis only needs to be performed if screening urinalysis was performed more than 7 days prior to 
cycle 1 day 1.  Microscopic analysis and/or urine protein creatinine ratio or 24-hour urine protein collection should be performed as clinically indicated. 
11. TRC105 Administration: Intravenous TRC105 diluted in normal saline will be administered every 7 days during Cycle 1.  The first weekly TRC105 dose 
will be given on cycle 1 day 1and split into two doses whereby 3 mg/kg is administered on cycle 1 day 1 and the balance is administered on cycle 1 day 4.  
The entire dose of TRC105 (10 mg/kg) is then given on cycle 1 day 8, cycle 1 day 15 and cycle 1 day 22. Starting on cycle 2 day 1 and beyond, TRC105 will 
be administered every two weeks on days 1 and 15. See Section 6.1.6 for specific TRC105 administration guidelines. 
12. Pazopanib Dosing: Oral pazopanib will be dosed once daily at 800 mg starting on cycle 1 day 1 in the absence of toxicity on days 1-28 of each 28 day cycle 
according to the pazopanib package insert.  Dose reductions are allowed based on individual patient tolerability.  See Section 6.2 for specific dosing 
guidelines.    
13. CT or MRI Tumor Imaging: Images of the chest, abdomen, and pelvis to be performed at screening, and on-study as outlined in the assessment table.  
Known areas of disease should be consistently followed throughout the study.  Assessments should be performed whenever disease progression is suspected.  
Allowable window for tumor imaging studies is +/- 7 days. Brain and bone scans are to be performed if metastases are suspected at screening or during study 
therapy.  Color photography with caliper or ruler measurements should be used to assess cutaneous tumors (target lesions). 

IND 131053                                      105SAR101 Protocol Amendment #7, July 22, 2016 
5.3.5.2 Clinical Protocol  
 
 
 
Page 55 of 111 
 
14. 12-Lead ECG: Single tracing 12-lead ECG will be performed at screening and at the time-points indicated in the Schedule of Assessments (pre-dose). If the 
patient develops an arrhythmia, the ECG should be repeated on day 1 of each subsequent cycle. For a QTc >500 ms, a repeat ECG for confirmation and 
evaluation of the ECG by a cardiologist is recommended.   Additional ECGs may be performed on study as clinically indicated.  
15. Concomitant Medications and Treatments: Concomitant medications and treatments will be recorded from 28 days prior to the start of study treatment 
and up to 28 days following the last dose of study treatment. Required TRC105 premedications should be recorded on TRC105 premedications CRF. 
16. Adverse Events: Patients must be followed for adverse events from the first day of treatment with pazopanib or TRC105 study drug until at least 28 days 
after the last dose of TRC105 or pazopanib study drug treatment, or until all serious or TRC105 related toxicities have resolved or are determined to be 
“chronic” or “stable”, whichever is later.  "Baseline-signs and symptoms” will be recorded from the date of informed consent on corresponding case report 
forms. Any serious AE that is possibly related to TRC105 occurring from the time of first TRC105 dose or at any point after the reporting period must be 
promptly reported to TRACON.  
17. TRC105 and Pazopanib Pharmacokinetics Trough Concentration: A 5 mL blood sample each for TRC105 and pazopanib pharmacokinetics to be 
collected at the time-points indicated in the Schedule of Assessments, prior to starting the TRC105 infusion and prior to pazopanib dosing.  Samples will be 
stored at approximately -70°C. Samples will be batch shipped as indicated in the laboratory manual to a third-party laboratory. See separate laboratory guide 
for further collection and shipment information. Additional PK samples may also be collected at the time of unexpected clinical events.  
18. Anti-product antibodies: 5 mL blood sample will be collected to assess anti-product antibodies at the time-points indicated in the schedule of assessments 
and stored at approximately -70°C to be analysed by a central laboratory. Samples will be batch shipped as indicated in the laboratory manual to a third-party 
laboratory. See separate laboratory guide for further collection and shipment information. Additional anti-product antibody samples may also be collected at 
the time of unexpected clinical events. 
19. Protein Biomarkers: One 10 mL purple top (K3EDTA) tube will be collected at the time-points indicated in the schedule of assessments and stored at 
approximately -70°C to be analysed by a central laboratory. See separate laboratory guide for further collection and shipment information. 
20. Archival Tumor Tissue: Archival specimens (formalin-fixed, paraffin-embedded) are required of the primary cancer and/or metastatic cancer specimen for 
each study participant. It is preferable that the entire paraffin block be submitted, but if this is not feasible, then at least 20 unstained slides are requested for 
immunohistochemical analysis (sections of 5 microns are preferred).  See separate laboratory guide for further collection and shipment information. 
21. Cycle 3+ Treatment: Patients who demonstrate a response of CR, PR or SD will be eligible for additional treatment until progression. 
22. Follow-up:  The follow-up visit should occur 28 days following the last dose of TRC105. The allowable visit window is +/- 7 days.  
23. Allowable window for each visit within the cycle is +/- 2 days unless otherwise stated. 
 
 
 
 
 
 
 
 

IND 131053                                      105SAR101 Protocol Amendment #7, July 22, 2016 
5.3.5.2 Clinical Protocol  
 
 
 
Page 56 of 111 
 
Table 6: 
Schedule of Assessments Phase 2 Soft Tissue Sarcoma 
Protocol Activities 
Screening 
Cycle 1 [23] 
Cycle 2+ [21] [23] 
End 
of 
Stud
y 
[3] 
28 Day 
Follow-up 
[22] 
Day -28 
Day 1  
[1] 
Day 4 
[1] 
Day 
8 
[1] 
Day 15  
[1] 
Day 
22 
[1] 
Day 1  
[1] 
Day 8  
[1] 
Day 
15 
[1] 
Day 
22 
[1] 
Baseline Documentation 
  
  
  
  
  
  
  
  
  
  
  
  
Informed Consent [4] 
X 
  
  
  
  
  
  
  
  
  
  
  
Medical/Oncology History [5] 
X 
  
  
  
  
  
  
  
  
  
  
  
Baseline Signs and Symptoms [5] 
X 
 
 
 
 
 
 
 
 
 
 
 
Physical Examination [6] 
X 
X 
  
  
  
  
X 
  
  
  
X 
  
Vital Signs [7] 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
  
Laboratory Studies 
  
  
  
  
  
  
  
  
  
  
  
  
Hematology [8] 
X+Fe 
X+Fe 
  
  
X 
  
X 
  
X 
  
X 
  
Coagulation [8] 
X 
X 
  
  
  
  
  
  
  
  
 
  
Blood Chemistry [8] 
X+TSH 
X+TSH 
  
  
X 
  
X + 
TSH 
  
 
  
X + 
TSH 
  
Pregnancy Test [9] 
X 
 X 
  
  
  
  
  
  
  
  
  
 X 
Urinalysis [10] 
X 
X 
  
  
  
  
X 
  
 
  
X 
  
Treatment w/ Study Drug 
  
  
  
  
  
  
  
  
  
  
  
  
TRC105 Dosing [11] 
  
X Split  
X Split  
X 
X  
X  
X  
X  
X  
X  
  
  
Pazopanib [12] 
 
Daily 
 
 
 
 
 
 
 
 
 
 
Tumor Assessments 
  
  
  
  
  
  
  
  
  
  
  
  
CT or MRI Scans [13] 
X 
  
  
  
  
 
  
  
  
Even 
Cycles 
X 
  
Other Clinical Assessments 
  
  
  
  
  
  
  
  
  
  
  
  
12-Lead ECG [14] 
X 
X  
  
  
 X  
  
 
  
  
  
X  
  
Concomitant 
Medications/Treatments [15] 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
Adverse Events [16] 
 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
Special Laboratory Assessments 
  
  
  
  
  
  
  
  
  
  
  
  
Anti-Product Antibody [17] 
 
X 
 
 
 
 
Even 
Cycles 
 
 
 
X 
X 
Trough PK [18] 
 
X 
 
 
 
 
Even 
Cycles 
 
 
 
X 
X 
Protein Biomarkers [19] 
 
X 
 
 
 
 
Even 
Cycles 
 
 
 
X 
 
Archival Tumor Tissue [20] 
X 
 
 
 
 
 
 
 
 
 
 
 

IND 131053                                      105SAR101 Protocol Amendment #7, July 22, 2016 
5.3.5.2 Clinical Protocol  
 
 
 
Page 57 of 111 
 
Schedule of Assessments Footnotes Phase 2 Soft Tissue Sarcoma 
1. Days of Treatment with TRC105: All assessments should be performed prior to the TRC105 infusion unless otherwise indicated.  Each cycle is 28 days in 
duration.  
2. Cycle 1 day 1: Hematology (including iron studies), blood chemistry (including TSH testing), urinalysis, physical examination, ECG and pregnancy test not 
required if acceptable screening assessment is performed within 7 days prior to the start of treatment on cycle 1 day 1.  
3. End of Study: The end of study visit should generally occur within 7 days (+/- 1 day) of the last dose of TRC105. Assessments other than pharmacokinetics, 
immunogenicity and protein biomarkers do not need to be repeated if performed within the previous 2 weeks (previous 8 weeks for radiologic tumor 
assessments). Follow-up visits should occur 28 days following the last dose of TRC105 study drug as outlined in the Schedule of Assessments.  
4. Informed Consent: Must be obtained prior to undergoing any study specific procedure and may occur prior to the 28-day screening period. 
5. Medical and Oncologic History,Demographics and Baseline Signs and Symptoms: All information related to prior anticancer treatment should be recorded. 
Significant medical history and baseline signs and symptoms should be captured from the date of informed consent.  
6. Physical Examination: Examination of major body systems and ECOG performance status.  
7.   Vital Signs: Heart rate, temperature, blood pressure, respiratory rate, weight. Assessment during TRC105 Infusions: Vital signs are to be assessed pre-infusion 
(i.e., within 30 minutes of starting the infusion) every 30 minutes during the infusion (+/- 15 minutes) and at the end of the infusion (i.e. within 30 minutes after 
completing the infusion). Vital signs should be monitored more frequently and/or beyond the completion of the infusion if medically indicated (e.g. if the patient 
experiences an infusion reaction that has not yet resolved).   
8. Hematology, Chemistry & Coagulation:  Testing to be performed locally.  Thyroid stimulating hormone to be tested at screening, day 1 of each cycle and at 
the end of study visit.  Cycle 1 day 1 assessments only need to be performed if screening assessments were performed more than 7 days prior to cycle 1 day 1.  
Iron studies (FE) to be performed according to the schedule of assessments and as clinically indicated during the study.  Lab assessments may be performed 
within 3 days prior to TRC105 dosing. In addition to the assessments scheduled for the clinical trial, patients should undergo assessment as appropriate to ensure 
safe treatment. See Section 8.1.1.1 for specific panel collection requirements.  
9. Pregnancy Test: Testing to be performed locally. All female patients of childbearing potential must have a negative serum or urine pregnancy test within 7 days 
of cycle 1 day 1 and 28 days following the last dose of TRC105. 
10. Urinalysis: To be performed locally. Cycle 1 day 1 urinalysis only needs to be performed if screening urinalysis was performed more than 7 days prior to cycle 1 
day 1.  Microscopic analysis and/or urine protein creatinine ratio or 24-hour urine protein collection should be performed as clinically indicated. 
11. TRC105 Administration: Intravenous TRC105 diluted in normal saline will be administered every 7 days.  The first weekly TRC105 dose will be given on cycle 
1 day 1and split into two doses whereby 3 mg/kg is administered on cycle 1 day 1 and the balance is administered on cycle 1 day 4.  The entire weekly dose of 
TRC105 (10 mg/kg) is then given on cycle 1 day 8 and weekly thereafter. See Section 6.1.6 for specific TRC105 administration guidelines. 
• 
Upon completion of enrollment of phase 1b dose levels 3 and notification by the sponsor, active patients that are past cycle 2, may be 
switched to an every 2 week dosing schedule at the every 2 week dosing MTD. 
12. Pazopanib Dosing: Oral pazopanib will be dosed once daily at 800 mg starting on cycle 1 day 1 in the absence of toxicity on days 1-28 of each 28 day cycle 
according to the pazopanib package insert.  Dose reductions are allowed based on individual patient tolerability beginning with cycle 1. See Section 6.2 for 
specific dosing guidelines.   Pazopanib should be dosed following PK collection on cycle 1 day 1 and day 1 of even cycles.  
13. CT or MRI Tumor Imaging: Images of the chest, abdomen, and pelvis to be performed at screening, and on-study as outlined in the assessment table.  Known 
areas of disease should be consistently followed throughout the study.  Assessments should be performed whenever disease progression is suspected.  Allowable 
window for tumor imaging studies is +/- 7 days. Brain and bone scans are to be performed if metastases are suspected at screening or during study therapy.  Color 
photography with caliper or ruler measurements, when possible, should be used to assess cutaneous tumors (target lesions). 

IND 131053                                      105SAR101 Protocol Amendment #7, July 22, 2016 
5.3.5.2 Clinical Protocol  
 
 
 
Page 58 of 111 
 
14. 12-Lead ECG: Single tracing 12-lead ECG will be performed at screening and at the time-points indicated in the Schedule of Assessments (pre-dose). If the 
patient develops an arrhythmia, the ECG should be repeated on day 1 of each subsequent cycle. For a QTc >500 ms, a repeat ECG for confirmation and 
evaluation of the ECG by a cardiologist is recommended.  Additional ECGs may be performed on study as clinically indicated.  
15. Concomitant Medications and Treatments: Concomitant medications and treatments will be recorded from 28 days prior to the start of study treatment and up 
to 28 days following the last dose of study treatment. Required TRC105 premedications should be recorded on TRC105 premedications CRF. 
16. Adverse Events: Patients must be followed for adverse events from the first day of treatment with pazopanib or TRC105 study drug until at least 28 days after the 
last dose of TRC105 or pazopanib study drug treatment, or until all serious or TRC105 related toxicities have resolved or are determined to be “chronic” or 
“stable”, whichever is later.  "Baseline-signs and symptoms” will be recorded from the date of informed consent on corresponding case report forms. Any serious 
AE that is possibly related to TRC105 occurring from the time of first TRC105 dose or at any point after the reporting period must be promptly reported to 
TRACON.  
17. Anti-Product Antibodies: 5 mL blood sample will be collected to assess Anti-Product Antibodies at the time-points indicated in the schedule of assessments and 
stored at approximately -70°C to be analysed by a central laboratory. Samples will be batch shipped as indicated in the laboratory manual to a third-party 
laboratory. See separate laboratory guide for further collection and shipment information. Additional Anti-Product Antibody samples may also be collected at the 
time of unexpected clinical events. 
18. TRC105 and Pazopanib Pharmacokinetics Trough Concentration: A 5 mL blood sample each for TRC105 and pazopanib pharmacokinetics to be collected at 
the time-points indicated in the Schedule of Assessments, prior to starting the TRC105 infusion and prior to pazopanib dosing.  Samples will be stored at 
approximately -70°C. Samples will be batch shipped as indicated in the laboratory manual to a third-party laboratory. See separate laboratory guide for further 
collection and shipment information. Additional PK samples may also be collected at the time of unexpected clinical events.  
19. Protein Biomarkers: One 10 mL purple top (K3EDTA) tube will be collected at the time-points indicated in the schedule of assessments and stored at 
approximately -70°C to be analysed by a central laboratory. See separate laboratory guide for further collection and shipment information. 
20. Archival Tumor Tissue: Archival specimens (formalin-fixed, paraffin-embedded) are required of the primary cancer and/or metastatic cancer specimen for each 
study participant. It is preferable that the entire paraffin block be submitted, but if this is not feasible, then at least 20 unstained slides are requested for 
immunohistochemical analysis (sections of 5 microns are preferred).  See separate laboratory guide for further collection and shipment information. 
21. Cycle 2+ Treatment: Patients who demonstrate a response of CR, PR or SD will be eligible for additional treatment until progression. 
22. Follow-up:  The follow-up visit should occur 28 days following the last dose of TRC105. The allowable visit window is +/- 7 days.  
23. Allowable window for each visit within the cycle is +/- 2 days unless otherwise stated. 
 
 
 
 
 
 
 
 

IND 131053                                      105SAR101 Protocol Amendment #7, July 22, 2016 
5.3.5.2 Clinical Protocol  
 
 
 
Page 59 of 111 
 
Table 7: 
Schedule of Assessments Phase 2 Angiosarcoma Cohorts 1 &2  
Protocol Activities 
Screening 
Cycle 1 [23] 
Cycle 2 [23] 
Cycle 3+ [21] [23] 
End 
of 
Study 
[3] 
28 
Day 
Follow
-up 
[22] 
Day -28 
Day 1 
[1] [2]  
Day4 
 [1] 
Day 
8 
[1] 
Day 
15  
[1] 
Day 
22 
[1] 
Day 
1 
[1] 
Day 
8 
[1] 
Day 
15  
[1] 
Day 
22 
[1] 
Day 1  
[1] 
Day 
8  
[1] 
Day 
15 
[1] 
Day 
22 
[1] 
Baseline Documentation 
  
 
 
  
 
 
  
  
  
  
  
  
  
  
  
  
Informed Consent [4] 
X 
 
 
  
 
 
  
  
  
  
  
  
  
  
  
  
Medical/Oncology History [5] 
X 
 
 
  
 
 
  
  
  
  
  
  
  
  
  
  
Baseline Signs and Symptoms [5] 
X 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Physical Examination [6] 
X 
X 
 
 
 
 
 X 
  
  
  
X 
  
  
  
X 
  
Vital Signs [7] 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
  
Laboratory Studies 
  
 
 
  
 
 
  
  
  
  
  
  
  
  
  
  
Hematology [8] 
X+Fe 
X 
 
 
X 
 
 X 
  
X 
  
X 
  
X 
  
X 
  
Coagulation [8] 
X 
X 
 
 
 
 
  
  
  
  
  
  
  
  
 
  
Blood Chemistry [8] 
X+TSH 
X + 
TSH 
 
  
X 
 
 X + 
TSH 
  
 
  
X + 
TSH 
  
 
  
X + 
TSH 
  
Pregnancy Test [9] 
Day -7 
 
 
  
 
 
X 
  
  
  
X 
  
  
  
  
 X 
Urinalysis [10] 
X 
X 
 
 
 
 
 X 
  
  
  
X 
  
 
  
X 
  
Treatment w/ Study Drug 
  
 
 
  
 
 
  
  
  
  
  
  
  
  
  
  
TRC105 WEEKLY Dosing [11] 
  
X Split 
X Split 
X  
X 
X 
X  
X 
X  
X 
X  
X 
X  
X  
  
  
*TRC105 EVERY 2 WEEK 
Dosing [11] 
  
X Split 
X Split 
X  
X 
X 
X  
 
X  
 
X  
 
X  
 
 
 
Pazopanib for Angiosarcoma 
cohort 1 [12] 
 
 
DAILY at time of disease 
progression  on single-agent 
TRC105 
 
 
Pazopanib for Angiosarcoma 
cohort 2 [12] 
 
Daily starting on Cycle 1 Day 1 
 
 
Tumor Assessments 
  
   
 
 
CT or MRI Scans [13] 
X 
Every 6 weeks from cycle 1 day 1 
X 
  
Other Clinical Assessments 
  
  
  
  
12-Lead ECG [14] 
X 
X 
 
  
X 
 
  
  
  
  
 
  
  
  
X  
  
Concomitant 
Medications/Treatments [15] 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
Adverse Events [16] 
 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
Special Laboratory Assessments 
  
  
  
  
  
Trough PK  [17] 
  
 
X 
X 
X 
 
 X 
  
X 
  
X &  
Even 
Cycles 
  
  
  
 X 
 X 
TRC105 Peak PK [17] 
 
X 
X 
X 
 
 
 
 
 
 
 
 
 
 
 
 
Anti-product antibodies [18] 
 
X 
 
 
 
 
X 
 
 
 
Even 
Cycles 
 
 
 
X 
X 
Protein Biomarkers [19] 
 
 
X 
 
 
 
 
X 
 
 
 
Even 
Cycles 
 
 
 
X 
 
Archival Tumor Tissue [20] 
X 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

IND 131053                                      105SAR101 Protocol Amendment #7, July 22, 2016 
5.3.5.2 Clinical Protocol  
 
 
 
Page 60 of 111 
 
* TRC105 may be administered every two weeks starting with cycle 2 day 1 once it is determined that the every two week dosing MTD has not been exceeded. SPONSOR WILL 
SEND NOTIFICATION TO PROCEED WITH EVERY TWO WEEK DOSING.  TRC105 will be administered weekly during cycle 1. The first weekly TRC105 dose will be split into two 
doses whereby 3 mg/kg is administered on cycle 1 day 1 and the balance is administered on cycle 1 day 4. Starting on cycle 2 day 1 and beyond, TRC105 may be administered every 
two weeks on days 1 and 15 at the every two-week dosing MTD. See Section 6.1.6  
Schedule of Assessments Footnotes Phase 2 Angiosarcoma Cohort  
1. Days of Treatment with TRC105: All assessments should be performed prior to the TRC105 infusion unless otherwise indicated.  Each cycle is 28 days in 
duration.  
2. Cycle 1 day 1: Hematology (including iron studies), blood chemistry (including TSH testing), urinalysis, physical examination, ECG and pregnancy test not 
required if acceptable screening assessment is performed within 7 days prior to the start of treatment on cycle 1 day 1.  
3. End of Study: The end of study visit should generally occur within 7 days (+/- 1 day) of the last dose of TRC105. Assessments other than 
pharmacokinetics, immunogenicity and protein biomarkers do not need to be repeated if performed within the previous 2 weeks (previous 8 weeks for 
radiologic tumor assessments). If the patient permanently discontinues all study therapy due to AE, patient decision, investigator decision or any other 
reason the end of study assessments should be completed.  Note that in this cohort, disease progression refers to disease progression while receiving 
TRC105 plus pazopanib combination therapy. Follow-up visits should occur 28 days following the last dose of TRC105 study drug as outlined in the 
Schedule of Assessments.  
4. Informed Consent: Must be obtained prior to undergoing any study specific procedure and may occur prior to the 28-day screening period. 
5. Medical and Oncologic History, Demographics and Baseline Signs and Symptoms: All information related to prior anticancer treatment should be 
recorded. Significant medical history and baseline signs and symptoms should be captured from the date of informed consent.  
6. Physical Examination: Examination of major body systems and ECOG performance status.  
7. Vital Signs: Heart rate, temperature, blood pressure, respiratory rate, weight. Assessment during TRC105 Infusions: Vital signs are to be assessed pre-
infusion (i.e., within 30 minutes of starting the infusion) every 30 minutes during the infusion (+/- 15 minutes) and at the end of the infusion (i.e. within 30 
minutes after completing the infusion). Vital signs should be monitored more frequently and/or beyond the completion of the infusion if medically indicated 
(e.g. if the patient experiences an infusion reaction that has not yet resolved).  If TRC105 administration is switched to every two week dosing, clinic visits 
are required only on days of TRC105 infusion (i.e., days 1 and 15 of each every two week administration cycle). 
8. Hematology, Chemistry & Coagulation:  Testing to be performed locally.  Thyroid stimulating hormone to be tested at screening, day 1 of each cycle and 
at the end of study visit.  Cycle 1 day 1 assessments only need to be performed if screening assessments were performed more than 7 days prior to cycle 1 
day 1.  Iron studies (FE) to be performed according to the schedule of assessments and as clinically indicated during the study.  Lab assessments may be 
performed within 3 days prior to TRC105 dosing. In addition to the assessments scheduled for the clinical trial, patients should undergo assessment as 
appropriate to ensure safe treatment. See Section 8.1.1.1 for specific panel collection requirements.  
9. Pregnancy Test: Testing to be performed locally. All female patients of childbearing potential must have a negative serum or urine pregnancy test within 7 
days of cycle 1 day 1, day 1 of every cycle starting with cycle 2, and 28 days following the last dose of TRC105. 
10. Urinalysis: To be performed locally. Cycle 1 day 1 urinalysis only needs to be performed if screening urinalysis was performed more than 7 days prior to 
cycle 1 day 1.  Microscopic analysis and/or urine protein creatinine ratio or 24-hour urine protein collection should be performed as clinically indicated. 
11. TRC105 Administration: Intravenous TRC105 diluted in normal saline will be administered as outlined in the assessments table.  See Section 6.1.6 for 
specific TRC105 administration guidelines. 
• 
Angiosarcoma cohort 1: The first weekly TRC105 dose will be given on cycle 1 day 1and split into two doses whereby 3 mg/kg is 
administered on cycle 1 day 1 and the balance is administered on cycle 1 day 4.  The entire weekly dose of TRC105 (10 mg/kg) is then given 
on cycle 1 day 8 and weekly thereafter. 

IND 131053                                      105SAR101 Protocol Amendment #7, July 22, 2016 
5.3.5.2 Clinical Protocol  
 
 
 
Page 61 of 111 
 
o 
Active patients that are past cycle 1, may be switched to an every 2 week dosing schedule at a TRC105 dose of 15 mg/kg every 
2 weeks (day 1 and day 15 of each 28 day cycle) once sponsor sends notification to proceed with every two week dosing.  
• 
Angiosarcoma cohort 2: TRC105 will be administered at a dose of 10 mg/kg weekly during cycle 1. The first weekly TRC105 dose (10 
mg/kg) will be split into two doses whereby 3 mg/kg is administered on cycle 1 day 1 and the balance is administered on cycle 1 day 4. Starting 
on cycle 2 day 1 and beyond, TRC105 will be administered every two weeks on days 1 and 15 at a dose of 15 mg/kg. 
12. Pazopanib Dosing: Angiosarcoma cohort 1--Oral pazopanib will be dosed orally at 800 mg starting at the time of disease progression on TRC105 single-
agent and once daily thereafter in the absence of toxicity.  Dose reductions are allowed based on individual patient tolerability beginning with cycle 1. 
Angiosarcoma cohort 2--Oral pazopanib will be given orally at 800 mg starting cycle 1 day 1 and once daily thereafter in the absence of toxicity.  See 
Section 6.2 for specific dosing guidelines.  Pazopanib should be dosed following PK collection on days when PK sampling is required as noted in the 
scheduled of assessments.   
13. CT or MRI Tumor Imaging: Images of the chest, abdomen, and pelvis to be performed at screening, and on-study as outlined in the assessment table.  
Known areas of disease should be consistently followed throughout the study.  Assessments should be performed whenever disease progression is suspected.  
Allowable window for tumor imaging studies is +/- 7 days. Brain and bone scans are to be performed if metastases are suspected at screening or during study 
therapy.  Color photography with caliper or ruler measurements, when possible, should be used to assess cutaneous tumors (target lesions). 
14. 12-Lead ECG: Single tracing 12-lead ECG will be performed at screening and at the time-points indicated in the Schedule of Assessments (pre-dose). If the 
patient develops an arrhythmia, the ECG should be repeated on day 1 of each subsequent cycle. For a QTc >500 ms, a repeat ECG for confirmation and 
evaluation of the ECG by a cardiologist is recommended.  Additional ECGs may be performed on study as clinically indicated.  
15. Concomitant Medications and Treatments: Concomitant medications and treatments will be recorded from 28 days prior to the start of study treatment 
and up to 28 days following the last dose of study treatment. Required TRC105 premedications should be recorded on TRC105 premedications CRF. 
16. Adverse Events: Patients must be followed for adverse events from the first day of treatment with pazopanib or TRC105 study drug until at least 28 days 
after the last dose of TRC105 or pazopanib study drug treatment, or until all serious or TRC105 related toxicities have resolved or are determined to be 
“chronic” or “stable”, whichever is later.  "Baseline-signs and symptoms” will be recorded from the date of informed consent on corresponding case report 
forms. Any serious AE that is possibly related to TRC105 occurring from the time of first TRC105 dose or at any point after the reporting period must be 
promptly reported to TRACON.  
17. TRC105 and Pazopanib Pharmacokinetics Trough and TRC105 Peak Concentration: A 5 mL blood sample each for TRC105 and pazopanib 
pharmacokinetics to be collected at the time-points indicated in the Schedule of Assessments. Trough samples should be collected prior to starting the 
TRC105 infusion and prior to pazopanib dosing (once pazopanib dosing begins for angiosarcoma cohort 1).  TRC105 peak samples to be collected within 10 
minutes of the completion of the TRC105 infusion on the days specified in the schedule of assessments. Samples will be stored at approximately -70°C. 
Samples will be batch shipped as indicated in the laboratory manual to a third-party laboratory. See separate laboratory guide for further collection and 
shipment information. Additional PK samples may also be collected at the time of unexpected clinical events.  
18. Anti-Product Antibodies: 5 mL blood sample will be collected to assess Anti-Product Antibodies at the time-points indicated in the schedule of 
assessments and stored at approximately -70°C to be analysed by a central laboratory. Samples will be batch shipped as indicated in the laboratory manual to 
a third-party laboratory. See separate laboratory guide for further collection and shipment information. Additional Anti-Product Antibody samples may also 
be collected at the time of unexpected clinical events. 
19. Protein Biomarkers: One 10 mL purple top (K3EDTA) tube will be collected at the time-points indicated in the schedule of assessments and stored at 
approximately -70°C to be analysed by a central laboratory. See separate laboratory guide for further collection and shipment information. 
20. Archival Tumor Tissue: Archival specimens (formalin-fixed, paraffin-embedded) are required of the primary cancer and/or metastatic cancer specimen for 
each study participant. It is preferable that the entire paraffin block be submitted, but if this is not feasible, then at least 20 unstained slides are requested for 
immunohistochemical analysis (sections of 5 microns are preferred).  See separate laboratory guide for further collection and shipment information. 

IND 131053                                      105SAR101 Protocol Amendment #7, July 22, 2016 
5.3.5.2 Clinical Protocol  
 
 
 
Page 62 of 111 
 
21. Cycle 2+ Treatment: Angiosarcoma cohort 1 patients will be eligible for single-agent TRC105 until progression. At the time of progression by RECIST 
1.1 on single-agent TRC105, patients will transition to treatment with the combination of TRC105 and pazopanib. Angiosarcoma cohort 2 patients will be 
eligible for treatment with the combination until progression. 
22. Follow-up:  The follow-up visit should occur 28 days following the last dose of TRC105. The allowable visit window is +/- 7 days.  
23. Allowable window for each visit within the cycle is +/- 2 days unless otherwise stated. 
 
 

IND 131053                                      105SAR101 Protocol Amendment #7, July 22, 2016 
5.3.5.2 Clinical Protocol  
 
 
 
Page 63 of 111 
 
5. 
SELECTION AND WITHDRAWAL OF PATIENTS 
For the Phase 1b dose levels -1, 1, 2 and expansion cohort 1, prior to administration of TRC105 
on cycle 2 day 1, all study patients must maintain eligibility criteria indicating adequate organ 
function, medical status and ECOG performance status (i.e., all patients must maintain inclusion 
criterion #6, 8, 9 and 10 and exclusion criteria #8-21 to continue into cycle 2). 
5.1. 
Patient Inclusion Criteria 
1. Histologically confirmed unresectable soft tissue sarcoma (i.e., non-GIST, non-
adipocytic) that has progressed following treatment with chemotherapy. Prior pazopanib 
is allowed if the drug was not discontinued for toxicity (Phase 1b only) 
2. Histologically confirmed metastatic soft tissue sarcoma (i.e., non-GIST, non-adipocytic) 
that has progressed by RECIST following treatment with anthracycline chemotherapy.  
Patients may have received up to four lines of systemic therapy for metastatic disease and 
no more than two lines of combination treatment ( Phase 2 only) 
3. Histologically confirmed locally advanced (e.g. unresectable) or metastatic angiosarcoma 
that has progressed following treatment with prior systemic therapy. Progression must be 
documented on or following the most recent systemic therapy. Prior pazopanib is allowed 
if the drug was not discontinued for toxicity (Phase 2 angiosarcoma cohort only)  
4. Measurable disease by RECIST 
5. Age of 12 years or older (patient must weigh ≥ 40 kg) 
6. ECOG performance status ≤ 1 
7. Resolution of all acute adverse events resulting from prior cancer therapies to NCI 
CTCAE grade ≤ 1 or baseline (except alopecia or neuropathy)   
8. Adequate organ function as defined by the following criteria: 
• Serum aspartate transaminase (AST; serum glutamic oxaloacetic transaminase 
[SGOT]) and serum alanine transaminase (ALT; serum glutamic pyruvic 
transaminase [SGPT]) ≤ 2.5 x upper limit of normal (ULN) or ≤ 5 x ULN in cases of 
liver metastases  
• Total serum bilirubin ≤ 1.5 times the upper limit of normal 
• Absolute neutrophil count (ANC) ≥ 1500/μL  
• Platelets ≥ 100,000/μL without transfusion support within the past 28 days 
• Hemoglobin ≥ 9.0 g/dL without transfusion support within the past 14 days 
(erythropoietin or darbepoietin permitted) 
• Serum creatinine ≤ 1.5 times the upper limit of normal or creatinine clearance  > 30 
mL/min by Cockcroft-Gault formula 
• INR  from 0.8 to 1.2 

IND 131053                                      105SAR101 Protocol Amendment #7, July 22, 2016 
5.3.5.2 Clinical Protocol  
 
 
 
Page 64 of 111 
 
9. Willingness and ability to consent for self to participate in study   
10. Willingness and ability to comply with scheduled visits, treatment plan, laboratory tests, 
and other study procedures 
11. Available archival tumor specimen of the soft tissue sarcoma that meets inclusion 
criterion #1, #2 or #3 
5.2. 
Exclusion Criteria   
1. Prior treatment with TRC105 
2. Prior treatment with a VEGFR TKI (including pazopanib) (Part 2 only) 
3. Current treatment on another therapeutic clinical trial 
4. Receipt of systemic anticancer therapy, including investigational agents, within 28 days 
of starting study treatment.  If anticancer therapy was given within 28 days of starting 
study treatment, patients may be included if 5 times the elimination half-life of the drug 
has passed.  
5. No major surgical procedure or significant traumatic injury within 6 weeks prior to study 
registration, and must have fully recovered from any such procedure; date of surgery (if 
applicable) or the anticipated need for a major surgical procedure within the next six 
months. Note: the following are not considered to be major procedures and are permitted 
up to  7 days before therapy initiation: Thoracentesis, paracentesis, port placement, 
laparoscopy, thorascopy, tube thoracostomy, bronchoscopy, endoscopic ultrasonographic 
procedures, mediastinoscopy, skin biopsies, incisional biopsies, imaging-guided biopsy 
for diagnostic purposes, and routine dental procedures 
6. Patients who have received wide field radiotherapy ≤ 28 days (defined as > 50% of 
volume of pelvic bones or equivalent) or limited field radiation for palliation < 14 days 
prior to study registration or those patients who have not recovered adequately from side 
effects of such therapy 
7. Uncontrolled chronic hypertension defined as systolic > 150 or diastolic > 90 despite 
optimal therapy (initiation or adjustment of BP medication prior to study entry is allowed 
provided that the average of 3 BP readings at a visit prior to enrollment is < 150/90 mm 
Hg) 
8. Significant ascites, pericardial or pleural effusion 
9. History of brain involvement with cancer, spinal cord compression, or carcinomatous 
meningitis, or new evidence of brain or leptomeningeal disease.  Patients with radiated or 
resected lesions are permitted, provided the lesions are fully treated and inactive, patients 
are asymptomatic, and no steroids have been administered for at least 28 days.  
10. Angina, MI, symptomatic congestive heart failure, cerebrovascular accident, transient 
ischemic attack, arterial embolism, pulmonary embolism, PTCA or CABG within the 
past 6 months. Deep venous thrombosis within 6 months, unless the patient is anti-
coagulated without the use of warfarin for at least 2 weeks. In this situation, low 
molecular weight heparin is preferred. 

IND 131053                                      105SAR101 Protocol Amendment #7, July 22, 2016 
5.3.5.2 Clinical Protocol  
 
 
 
Page 65 of 111 
 
11. Active bleeding or pathologic condition that carries a high risk of bleeding (e.g. 
hereditary hemorrhagic telangiectasia).  Patients who have been uneventfully anti-
coagulated with low molecular weight heparin are eligible. 
12. Thrombolytic use (except to maintain i.v. catheters) within 10 days prior to first day of 
study therapy 
13. Known active viral or nonviral hepatitis or cirrhosis 
14. History of hemorrhage or hemoptysis (> ½ teaspoon bright red blood) within 3 months of 
starting study treatment 
15. History of peptic ulcer within 3 months of treatment, unless treated for the condition and 
complete resolution has been documented by esophagogastroduodenoscopy (EGD) 
within 28 days of starting study treatment 
16. History of gastrointestinal perforation or fistula in the past 6 months, or while previously 
on antiangiogenic therapy, unless underlying risk has been resolved (e.g., through 
surgical resection or repair) 
17. Known human immunodeficiency virus (HIV) or acquired immunodeficiency syndrome 
(AIDS) related illness 
18. Receipt of a strong CYP3A4 inducer within 12 days prior to cycle 1 day 1 or a strong 
CYP3A4 inhibitor within 7 days prior to cycle 1 day 1 (Table 14) 
19. Pregnancy or breastfeeding.  Female patients must be surgically sterile (i.e.: 
hysterectomy) or be postmenopausal, or must agree to use effective contraception during 
the study and for 3 months following last dose of TRC105.  All female patients of 
reproductive potential must have a negative pregnancy test (serum or urine) within 7 days 
prior to first dose.  Male patients must be surgically sterile or must agree to use effective 
contraception during the study and for 3 months following last dose of TRC105.  The 
definition of effective contraception will be based on the judgment of the Principal 
Investigator or a designated associate.   
20. Other severe acute or chronic medical or psychiatric condition or laboratory abnormality 
that may increase the risk associated with study participation or may interfere with the 
interpretation of study results and, in the judgment of the Investigator, would make the 
patient inappropriate for this study 
5.3. 
Patient Withdrawal Criteria 
A patient should be withdrawn from study treatment if, in the opinion of the Investigator, it is 
medically necessary, or if it is the wish of the patient.  If a patient does not return for a scheduled 
visit, every effort should be made to contact the patient.  In any circumstance, every effort should 
be made to document patient outcome.  Data to be collected at the end of study visit are 
described in the Schedule of Assessments (Table 4, Table 5, Table 6 and Table 7).  Patients will 
be followed for at least 28 days after the last dose of TRC105 study drug for adverse events.  If 
the patient withdraws consent, no further evaluations should be performed, and no attempts 
should be made to collect additional data. In addition, patients will be withdrawn from treatment 
in the case of: 

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1. RECIST 1.1-defined disease progression. In cases where RECIST cannot be applied, 
progression should be based on unequivocal evidence of progressive disease sufficient to 
require a change in therapy. In the angiosarcoma cohort 1, disease progression for 
withdrawal refers to disease progression while receiving TRC105 plus pazopanib 
combination therapy. 
2. A need for surgery, radiation, or for other anticancer therapy not specified in the protocol. 
3. Unable to tolerate pazopanib during cycle 1 (phase 1b, dose levels -1, 1, 2 and expanded 
cohort 1 only). 
4. Lost to follow-up or noncompliant. 
5. Any TRC105 dose delay > 2 days between cycle 2 day 1 and cycle 2 day 28 (phase 1b 
only). 
6. Pregnancy.  Pregnant patients should be followed for the duration of the pregnancy and 
the outcome of the pregnancy should be documented. 
7. Arterial thrombosis of any grade (including cerebrovascular ischemia, cardiac 
ischemia/infarction, or peripheral or visceral arterial ischemia) or grade 3 or 4 venous 
thrombosis (including pulmonary embolism). 
8. Missed study drug treatment for > 8 consecutive weeks (i.e., both TRC105 and pazopanib 
dosing held). However, patients who cannot tolerate pazopanib or TRC105 therapy and 
who demonstrate a response of complete response (CR), partial response (PR) or stable 
disease (SD) with the combination and are thought to benefit from continued single agent 
therapy may continue on study on TRC105 or pazopanib alone.  

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6. 
TREATMENT OF PATIENTS 
6.1. 
Description of TRC105 Study Drug 
TRC105 is a genetically engineered human/murine chimeric monoclonal antibody directed 
against human CD105 found on the surface of proliferating endothelial cells.   
6.1.1. 
Composition of TRC105 
TRC105 is an IgG1, kappa immunoglobulin containing murine light- and heavy-chain variable 
region sequences and human constant region sequences.  TRC105 has an approximate molecular 
weight of 148 kDa.  
6.1.2. 
TRC105 Dose Level 
6.1.2.1. 
Phase 1b 
Each patient will be dosed with 6 (Dose Level -1), 8 (Dose Level 1) or 10 mg/kg (Dose Level 2),  
or 15 mg/kg (Dose Level 3) The maximum weight that should be used for purpose of dose 
calculation is 85 kg for women and 100 kg for men. Thus, the maximum dose that should be 
given to a woman at the 10 mg/kg dose is 850 mg and at the 15 mg/kg dose is 1275 mg, and the 
maximum dose that should be given to a man at the 10 mg/kg dose is 1000 mg and at the 15 
mg/kg dose is 1,500 mg. TRC105 is distributed according to lean body mass rather than overall 
body weight.  Patients who are overweight would be at risk for high serum levels of TRC105 if 
the doses were not capped.  Eighty-five kg for women and 100 kg for men represent accepted 
maximum lean body masses for the two genders.  
For dose levels -1, 1, 2, and expanded cohort 1the first weekly TRC105 dose will be given on 
cycle 2 day 1 following two, three, or four weeks of treatment with pazopanib alone. The first 
weekly dose will be split into two doses whereby 3 mg/kg will be administered on cycle 2 day 1 
and the balance will be administered on cycle 2 day 4).  The entire weekly dose of TRC105 (6, 8 
or 10 mg/kg) is then given on cycle 2 day 8 and weekly thereafter in combination with oral 
pazopanib.  
For dose levels 3and expanded cohort 2, TRC105 dosing will begin on cycle 1 day 1 with the 
first weekly dose split into two doses whereby 3 mg/kg is administered on cycle 1 day 1 and 7 
mg/kg is administered on cycle 1 day 4. The entire weekly dose of TRC105 at 10 mg/kg is then 
given on cycle 1 day 8 and weekly until cycle 2 day 1 when TRC105 is administered at 15 mg/kg 
every two weeks. The DLT evaluation period, for purposes of dose escalation, will be cycle 2. 
Each cycle will be 28 days in duration. 
6.1.2.2. 
Phase 2 
Each patient, including patients in the angiosarcoma cohort, will be dosed with 10 mg/kg up to a 
maximum dose of 850 mg of TRC105 for women and 1,000 mg of TRC105 for men (i.e., 85 
kg for women and 100 kg for men is the maximum weight that should be used for purposes 
of dose calculation on this study).  TRC105 is distributed according to lean body mass rather 
than overall body weight.  Patients who are overweight would be at risk for high serum levels of 
TRC105 if the doses were not capped.  85 kg for women and 100 kg for men represent accepted 

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5.3.5.2 Clinical Protocol  
 
 
 
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maximum lean body masses for the two genders. The first weekly TRC105 dose will be given on 
cycle 1 day 1. The first weekly dose will be split into two doses whereby 3 mg/kg will be 
administered on cycle 1 day 1 and the balance will be administered on cycle 1 day 4.  The entire 
weekly dose of TRC105 (10 mg/kg) is then given on cycle 1 day 8 and weekly thereafter.  Each 
cycle following will be 28 days in duration. 
TRC105 may be administered every two weeks starting with cycle 2 day 1, once it is 
determined that the every two week dosing MTD has not been exceeded. SPONSOR WILL 
SEND NOTIFICATION TO PROCEED WITH EVERY TWO WEEK DOSING.   
• TRC105 will be administered at 10 mg/kg weekly during cycle 1.  
o The first weekly TRC105 dose (10 mg/kg) will be split into two doses whereby 3 
mg/kg is administered on cycle 1 day 1 and the balance is administered on cycle 1 
day 4.  
• Starting on cycle 2 day 1 and beyond, TRC105 may be administered every two weeks on 
days 1 and 15 at 15 mg/kg. 
6.1.3. 
TRC105 Packaging and Labeling 
TRC105 may be provided in one or more of the following presentations. 
Phosphate Buffered Saline Formulation (7 mg TRC105/mL)  
210 mg TRC105/30 mL single-use vial 
20 mM L-Histidine/L-Histidine Monohydrochloride, 240 mM Trehalose, 0.01% Polysorbate 
20 Formulation (25 mg TRC105/mL) 
100 mg TRC105/4 mL single-use vial 
200 mg TRC105/8 mL single-use vial 
400 mg TRC105/16 mL single-use vial 
 
6.1.4. 
TRC105 Storage and Shipping 
TRC105 must be stored upright between 2 °C and 8 °C (36 °F to 46 °F) and protected from light. 
6.1.5. 
TRC105 Preparation 
TRC105 will be prepared in the pharmacy and diluted into normal saline using appropriate 
aseptic technique. TRC105 will be administered using an in-line 0.2 micron filter.  No 
incompatibilities between TRC105 and polyvinyl chloride or polyolefin bags have been 
observed.  Multiple vials will be required for a single dose.  The following formulae should be 
used to calculate the volume of TRC105 to be added to normal saline: 
• Patient weight (kg) × dose level (mg/kg) divided by TRC105 concentration (mg/mL) = 
volume of TRC105 (mL) to be administered. 
The volume of TRC105 that is to be administered can be rounded up or down to the nearest 1.0 
mL; in the case of an increment of 0.5 mL the volume should be rounded up. The maximum 

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weight that should be used for dose calculation in this study is 85 kg for women and 100 kg 
for men (note: there is not a weight restriction for enrollment purposes).  If the patient’s 
weight changes by > 10% during the study, the dose of TRC105 will be recalculated.  At that 
time a new baseline weight will be established such that subsequent weight changes by >10% 
from the new baseline weight would require further recalculation of the TRC105 dose. The 
calculated volume of TRC105 will be diluted with normal saline.  Appropriate judgment should 
be exercised in withdrawing an adequate amount of saline necessary to permit injection of the 
appropriate volume of antibody into a normal saline bag in accordance with the dose needed.  
The final TRC105 concentration must be between 0.6 mg/mL and 10 mg/mL.  The prepared 
TRC105 must be gently inverted several times in order to ensure a homogeneous solution. The 
diluted infusion solution of TRC105 should be used within 8 hours of preparation if stored at 
room temperature, or within 24 hours of dilution if stored at 2° to 8°C (36º to 46ºF). The 
expiration time should be labeled on the bag.  If the diluted infusion solution of TRC105 cannot 
be infused within 8 hours of preparation (i.e.: the prepared infusion is at room temperature for 
more than 8 hours), a second bag will be prepared that contains the balance of the planned dose 
that was not already delivered. The prepared solution should not be frozen. 
6.1.6. 
TRC105 Administration 
Patients should be encouraged to drink abundant fluid (e.g., two eight ounce glasses of water or 
juice) prior to the first treatment.  Intravenous hydration prior to and during therapy is left to the 
discretion of the Investigator, but should be considered for patients that may be volume depleted. 
The following TRC105 premedications should be administered 2 hours to 30 minutes prior to the 
start of each infusion:   
• Acetaminophen 650 mg p.o. x 1  
• Methylprednisolone 100 mg i.v. will be given prior to the cycle 1 day 1 and cycle 1 
day 4 infusions.  In addition, methylprednisolone will be given in the case of a delay 
of > 10 days between any two scheduled weekly doses or in the case of a delay of > 
17 days between any two scheduled every other week doses, or if the patient develops 
an infusion reaction > grade 2 during the immediate prior infusion.  
• Famotidine 20 mg i.v. or p.o. (or similar H2 blocker) x 1. Famotidine (or similar H2 
blocker) may be discontinued starting with Cycle 2 for weekly dosing , Cycle 3 for 
every two week dosing, in the absence of infusion reactions with the prior dose. 
• Cetirizine 10 mg i.v. or p.o. x 1 (or similar oral or intravenous antihistamine).  
Cetirizine (or similar oral or intravenous antihistimine) may be discontinued starting 
with Cycle 2 for weekly dsoing, Cycle 3 for every two week dosing, in the absence of 
infusion reactions with the prior dose. 
TRC105 infusions will begin 2 hours to 30 minutes following the completion of the TRC105 
premedication, including the methylprednisolone infusion.     
TRC105 will be administered intravenously utilizing an infusion pump.  TRC105 has been 
demonstrated to be compatible with polyethylene lined, non-DEHP infusion sets and polyvinyl 
chloride, non-DEHP infusion sets.  TRC105 is required to be administered with a 0.2 micron 
downstream filter.  The attachment of the infusion pump administration set to the i.v. bag and 

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transport of the TRC105 study drug to the patient will be performed as per standard study site 
procedures.   
Following the appropriate premedication regimen, the first weekly TRC105 dose will be split 
into two doses whereby 3 mg/kg is administered (on cycle 2 day 1 for the phase 1b portion dose 
levels -1, 1, 2, and expansion cohort 1  and cycle 1 day 1 for the phase 1b dose levels 3,  
expansion cohort 2 and phase 2 portion) over 4 hours (+/- 15 minutes) and the balance (e.g., 5 
mg/kg for Dose Level 1) is administered on day 4 over 2 hours (+/- 15 minutes).  Do not increase 
the infusion rate above 25 mg/min.  Thereafter, the full (e.g., 8 mg/kg for Dose Level 1) TRC105 
dose will be administered i.v. weekly over 1 hour (+/- 15 minutes) (e.g., the first full dose is 
given on cycle 2 day 8 for the phase 1b portion and cycle 1 day 8 for the phase 2 portion).   
Every two week infusions will begin on cycle 2 day 1 for phase 1b dose levels 3and expanded 
cohort 2. Patients must complete at least one 4 hour infusion without the development of any 
infusion reactions, in order to reduce the subsequent TRC105 infusion to 2 hours (+/- 15 
minutes) and complete a 2 hour infusion without the development of any infusion reactions in 
order to reduce subsequent TRC105 infusions to 1 hour (+/- 15 minutes).  See Table 8 Table 9 
for ideal dosing schemas.  Patients with infusion reactions of any kind should be managed 
appropriately (see Section 6.1.8 ) and are not permitted to reduce the duration of the next planned 
infusion. 
The rate of TRC105 infusion must not exceed 25 mg/min.  When the i.v. bag containing TRC105 
is empty, flush the i.v. line with a 20 mL normal saline.  The dose level, time of transfer to i.v. 
bag, and the infusion start and stop times must be recorded in the source documents. 
 
 
Table 8: 
TRC105 10 mg/kg Weekly Ideal Dosing Schema 
 
C1D1 C1D4 C1D8 C1D15 C1D22 C2D1+ 
TRC105 Dose (mg/kg) 
3  
7  
10  
10  
10  
10  
Infusion Duration (hours) 
4 
2 
1 
1 
1 
1 
Premedication 
Methylprednisolone (mg) 
100 
100 
0 
0 
0 
0 
Famotidine (mg) 
20 
20 
20 
20 
20 
0 
Cetirizine (mg) 
10 
10 
10 
10 
10 
0 
Acetaminophen (mg) 
650 
650 
650 
650 
650 
650 

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Table 9: 
TRC105 15 mg/kg Every Two Weeks Ideal Dosing Schema (Ph1b Dose Level 
3) 
 
C1D1 C1D4 C1D8 C1D15 C1D22 
C2D1 
C2D15 
C3D1+ 
TRC105 Dose (mg/kg) 
3  
7  
10  
10  
10  
15  
15  
15  
Infusion Duration (hours) 
4 
2 
1 
1 
1 
1 
1 
1 
Premedication 
Methylprednisolone (mg) 
100 
100 
0 
0 
0 
0 
0 
0 
Famotidine (mg) 
20 
20 
20 
20 
20 
20 
20 
0 
Cetirizine (mg) 
10 
10 
10 
10 
10 
10 
10 
0 
Acetaminophen (mg) 
650 
650 
650 
650 
650 
650 
650 
650 
6.1.7. 
TRC105 Dose Modification/Dose Delays 
In cycle 3 and beyond (and beginning in cycle 2 of the phase 2 portion), TRC105 dose reductions 
are allowed for grade 3 or 4 related adverse events that resolve to grade 1 or baseline (including 
anemia).  Treatment dose delays cannot exceed 8 consecutive weeks (i.e., both TRC105 and 
pazopanib dosing held). However, patients who cannot tolerate pazopanib or TRC105 therapy 
and who demonstrate a response of complete response (CR), partial response (PR) or stable 
disease (SD) with the combination and are thought to benefit from continued single agent 
therapy may continue on study on TRC105 or pazopanib alone per Section 5.3 of the protocol.  
 
 
 
 
 
 
 
 

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Table 10: 
Allowable TRC105 Dose Modifications 
Toxicity Attributed to 
TRC105 
Dose Adjustment for Next Dose of TRC105  
Dose Schedule/Level 
10 mg/kg weekly 
15 mg/kg every 2 weeks 
Grade 1 or 2 
Maintain Dose Level 
Maintain Dose Level 
Grade 3 or 4 
 
 
• 
1st appearance 
8 mg/kg weekly 
12 mg/kg every 2 weeks 
• 
2nd appearance 
6 mg/kg weekly 
10 mg/kg every 2 weeks 
• 
3rd appearance 
4 mg/kg weekly 
8 mg/kg every 2 weeks 
• 
4th appearance 
Discontinue TRC105 
treatment permanently 
Discontinue TRC105 treatment 
permanently* 
*After discussion with and agreement of the Sponsor, patients receiving TRC105 every two 
weeks have the option to return to weekly dosing at the lowest level (i.e., 4 mg/kg weekly), if 8 
mg/kg every 2 weeks is not tolerable (i.e., 4th appearance of a Grade 3 or 4 toxicity attributable 
to TRC105 occurs) and the investigator believes that the patient is receiving benefit from the 
treatment. 
Patients with arterial thrombosis or grade 3 or 4 venous thrombosis should be removed from 
study.  Patients with grade 1 or 2 venous thrombosis who require anticoagulation will have their 
TRC105 therapy interrupted.  TRC105 therapy may resume once the following criteria are met:   
• The patient is on a stable dose of heparin or low molecular weight heparin.  
• The patient has a platelet count > 100,000. 
• The patient has not had a hemorrhagic event of grade 2 or higher while on study. 
• The patient does not have a pathological condition that carries a high risk of bleeding 
(e.g., tumor involving major vessels). 
• The patient is benefiting from TRC105 therapy (no evidence of disease progression). 
 
TRC105 and pazopanib should be held for two weeks prior and for two weeks following surgical 
procedures. However, resumption of study treatment can be shorter (but no less than 7 days) or 
longer than two weeks based on clinical judgement of adequate wound healing and recovery 
from the procedure. For minor procedures (e.g., port placement), TRC105 (and pazopanib) 
should be held for at least 1 week prior and for at least 1 week after (or until adequate healing). 
 
TRC105 DOSING DELAY:  If a patient misses a scheduled weekly TRC105 dose and dosing 
is resumed ≥ 10 days after the last dose or if a patient misses a scheduled every 2 week dose and 
dosing is resumed ≥ 17 days after the last dose, premedication (including methylprednisolone) 
and TRC105 are to be administered as described in Table 11. Split dosing is not required. 
However, it is recommended that if the patient experienced a severe headache with a previous 

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infusion, the first TRC105 dose upon resumption should be administered over two days as was 
done for the initial dose, see Table 12.  
Table 11: 
TRC105 dosing after Dose Delay (with no split dosing) 
 
CxDy 
CxDy 
CxDy 
CxDy 
CxDy+ 
TRC105 Dose (mg/kg) 
10 or 15  10 or 15  10 or 15  
10 or 15  
10 or 15  
Infusion Duration 
(hours) 
4 
2 
1  
1  
1  
Premedication 
Methylprednisolone 
(mg) 
100 
0 
0 
0 
0 
Famotidine (mg) 
20 
20 
20 
20 
0 
Cetirizine (mg) 
10 
10 
10 
10 
0 
Acetaminophen (mg) 
650 
650 
650 
650 
650 
Note that acetaminophen should NOT be discontinued.  
 
Table 12: TRC105 Dosing after Dose Delay (with split dosing)  
 
Day 1 
resuming 
TRC105 
 3 days 
later 
Weekly or 
every 2 weeks 
from Day 1 
Weekly or 
every 2 
weeks  
CxDy+ 
TRC105 Dose (mg/kg) 
3   
7 or 12  
10 or 15  
10 or 15  
10 or 15  
Infusion Duration (hours) 
4 
2 
1  
1  
1  
Premedication 
Methylprednisolone (mg) 
100 
100 
0 
0 
0 
Famotidine (mg) 
20 
20 
20 
20 
0 
Cetirizine (mg) 
10 
10 
10 
10 
0 
Acetaminophen (mg) 
650 
650 
650 
650 
650 
Note that acetaminophen should NOT be discontinued.  

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The schedule of assessment should be followed with regards to visits, labs, and any other 
required assessments even if TRC105 dosing is held. 
6.1.8. 
Management of TRC105 Infusion Reactions 
If a patient experiences a grade 2 or higher adverse reaction during infusion, the infusion should 
be interrupted and the patient treated accordingly.  Antipyretic, antihistamine, antiemetic, anti-
inflammatory, or other symptomatic medications including epinephrine may be administered as 
indicated.  For grade 2 and certain grade 3 infusion reactions, the infusion may be restarted at 
half of the previous rate if and when the infusion reaction has resolved, and then increased per 
patient tolerance to a maximum of 25 mg/min.  For grade 4 infusion reactions, the infusion 
should not be restarted and the patient should discontinue TRC105 treatment.  Infusion reactions 
will be recorded as AEs in the case report form.  Interventions should be documented as 
concomitant medications or concomitant treatments as appropriate.   
Table 13: 
Management of TRC105 Infusion Reactions 
Infusion Reaction 
Severity 
Recommended Management 
Grade 1 (mild) 
1. No intervention 
2. Continue infusion unless symptoms worsen 
Grade 2 (moderate) 
1. Interrupt infusion 
2. Treat with symptomatic medicationsa 
3. Resume infusion at half the previous rate when infusion-related 
symptoms improve to grade 1 or less. 
Grade 3 (severe) 
1. Interrupt infusion 
2. Treat with symptomatic medicationsa 
3. Monitor patient until infusion-related symptoms resolve, including 
hospitalization if necessary 
4. Discontinue TRC105 treatment unless other factors that contributed to 
the infusion reaction are identified and corrected 
Grade 4 (life-
threatening) 
1. Discontinue infusion 
2. Treat with symptomatic medicationsa 
3. Hospitalize patient 
4. Discontinue TRC105 treatment 
aSymptomatic medications may include but are not limited to diphenhydramine 50 mg i.v. and/or hydrocortisone 
100 mg i.v. (for fever, rash, hypoxia, or other hypersensitivity reactions), meperidine 50-100 mg i.v. (for shaking 
chills/rigors), oxygen by mask or nasal cannula (for hypoxia), epinephrine 0.5 mg i.m. (for hypotension or 
bronchospasm), albuterol inhaler or nebulizer (for bronchospasm), i.v. fluids (for hypotension), and ondansetron 
0.15 mg/kg i.v. (for nausea). 
6.1.9. 
TRC105 Study Drug Accountability 
The Investigator must maintain an accurate accounting of TRC105 supplied by TRACON.  
During the study, the following information must be recorded: 

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• Date of receipt, quantity and lot number of the TRC105 study drug received from 
TRACON 
• ID number of the patient to whom the product is dispensed 
• The date(s) and quantity of the product dispensed 
• Dates and quantity of product returned, lost or accidentally or deliberately destroyed 
Investigational Drug Accountability Logs should be maintained by the site and must be readily 
available for inspection. 
6.1.10. 
TRC105 Study Drug Handling and Disposal 
TRC105 must be stored upright between 2 °C and 8 °C (36 °F to 46 °F) and protected from light. 
The Investigator should not return clinical study materials to TRACON unless specifically 
instructed to do so by TRACON.  Used vials do not need to be maintained.  All expired vials of 
TRC105 should be retained until destruction is authorized by a TRACON representative.  The 
Site Pharmacist will be responsible for documenting the destruction (according to institutional 
requirements) of used or expired vials. 
6.2. 
Description of Pazopanib 
See pazopanib package insert [67].  
6.2.1. 
Composition of Pazopanib 
See pazopanib package insert [67].  
6.2.2. 
Pazopanib Dose Level 
Each patient, except for patients in the angiosarcoma cohort, will be dosed with 800 mg of 
pazopanib on cycle 1 day 1 and daily thereafter, in the absence of toxicity, once daily without 
food (at least 1 hour before or 2 hours after a meal) for each day of a 28 day cycle [67].  Note 
that in phase 1b dose levels 1, 2 and expanded cohort 1 only, cycle 1 will be 14 to 28 days in 
duration during which pazopanib will be dosed alone.  Pazopanib dose reductions are allowed 
based on individual patient tolerability, including during the initial 2 to 4 week period of the 
phase 1b when pazopanib is dosed in the absence of TRC105.  
Patients in the angiosarcoma cohort 1 will start pazopanib dosing at the time of disease 
progression on TRC105 single-agent. The starting pazopanib dose will be 800 mg daily in 
accordance with the package insert. Pazopanib dose reductions are allowed based on individual 
patient tolerability. 
6.2.3. 
Pazopanib Packaging and Labeling 
See pazopanib package insert [67]. 
6.2.4. 
Pazopanib Storage Handling and Disposal 
See pazopanib package insert [67]. 

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6.2.5. 
Pazopanib Dosing 
The recommended starting dose of pazopanib is 800 mg orally once daily without food (at least 1 
hour before or 2 hours after a meal). The dose of pazopanib will not exceed 800 mg. 
Do not crush tablets due to the potential for increased rate of absorption which may affect 
systemic exposure. 
If a dose is missed, it should not be taken if it is less than 12 hours until the next dose [67]. 
6.2.6. 
Pazopanib Dose Modification 
Dose increase or reduction is recommended based on individual safety and tolerability.  Decrease or 
increase should be in 200 mg steps based on individual tolerability.  The dose of pazopanib will 
not exceed 800 mg. If a dose is missed, it should not be taken if it is less than 12 hours until the 
next dose.  Patients who cannot tolerate pazopanib or TRC105 therapy and who demonstrate a 
response of complete response (CR), partial response (PR) or stable disease (SD) with the 
combination and are thought to benefit from continued single agent therapy may continue on 
study on TRC105 or pazopanib alone per Section 5.3 of the protocol. 
Hepatic Impairment: No dose adjustment is required in patients with mild hepatic impairment. In 
patients with moderate hepatic impairment, alternatives to pazopanib should be considered. If 
pazopanib is used in patients with moderate hepatic impairment, the dose should be reduced to 
200 mg per day. Pazopanib is not recommended in patients with severe hepatic impairment. 
• Patients with isolated ALT elevations between 3 X ULN and 8 X ULN may be continued 
on pazopanib with weekly monitoring of liver function until ALT return to Grade 1 or 
baseline. 
• Patients with isolated ALT elevations of >8 X ULN should have pazopanib interrupted 
until they return to Grade 1 or baseline. If the potential benefit for reinitiating treatment 
with pazopanib is considered to outweigh the risk for hepatotoxicity, then reintroduce 
pazopanib at a reduced dose of no more than 400 mg once daily and measure serum liver 
tests weekly for 8 weeks. Following reintroduction of pazopanib, if ALT elevations >3 X 
ULN recur, then pazopanib should be permanently discontinued. 
• If ALT elevations >3 X ULN occur concurrently with bilirubin elevations >2 X ULN, 
pazopanib should be permanently discontinued. Patients should be monitored until 
resolution. pazopanib is a UGT1A1 inhibitor. Mild, indirect (unconjugated) 
hyperbilirubinemia may occur in patients with Gilbert’s syndrome. Patients with only a 
mild indirect hyperbilirubinemia, known Gilbert’s syndrome, and elevation in ALT >3 X 
ULN should be managed as per the recommendations outlined for isolated ALT 
elevations. 
Blood pressure should be monitored and managed promptly using a combination of anti-
hypertensive therapy and dose modification of pazopanib (interruption and re-initiation at a 
reduced dose based on clinical judgment). 
Increased blood pressure should be treated promptly with standard anti-hypertensive therapy and 
dose reduction or interruption of pazopanib as clinically warranted. pazopanib should be 
discontinued if there is evidence of hypertensive crisis or if hypertension is severe and persistent 

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despite anti-hypertensive therapy and dose reduction. Approximately 1% of patients required 
permanent discontinuation of pazopanib because of hypertension [67].  
Interrupt pazopanib and dose reduce for 24-hour urine protein ≥3 grams; discontinue pazopanib 
for repeat episodes despite dose reductions. 
Institute appropriate anti-infective therapy promptly and consider interruption or discontinuation 
of pazopanib for serious infections. 
Interruption of therapy with pazopanib is recommended in patients undergoing surgical 
procedures [67]. TRC105 and pazopanib should be held for two weeks prior and for two weeks 
following surgical procedures. For minor procedures (e.g., port placement), TRC105 (and 
pazopanib) should be held for at least 1 week prior and for at least 1 week after (or until adequate 
healing). 
 
6.2.7. 
Pazopanib Drug Accountability 
Patients will be asked to return any unused tablets from the previous cycle for proper drug 
accountability and destruction according to institution guidelines. A new prescription will be 
dispensed for the following cycle.  
6.3. 
Concomitant Medications 
No other approved or investigational anticancer treatment will be permitted during the study 
period.  No other investigational drug may be used during treatment on this protocol, and 
concurrent participation in another clinical trial is not allowed. 
Patients who receive NSAIDs on study should also receive peptic ulcer disease (PUD) 
prophylaxis with an H2 or proton pump blocker. 
Narcotic analgesics, nonsteroidal anti-inflammatory drugs, and triptans (e.g. sumatriptan) may be 
offered as needed for relief of pain or headaches.  Antihistamines and decongestants may be 
offered for the treatment of sinus congestion. 
Packed red blood cell, colony stimulating factors, and platelet transfusions should be 
administered as clinically indicated.  
CYP3A4 Inhibitors: Avoid use of strong inhibitors (Table 14); co-administration of pazopanib 
with strong inhibitors of CYP3A4 increases pazopanib concentrations. Patients may not have 
received a strong CYP3A4 inhibitor within 7 days prior to cycle 1 day 1. If co-administration is 
warranted, reduce the dose of pazopanib to 400 mg.  
CYP3A4 Inducers: Consider an alternate concomitant medication with no or minimal enzyme 
induction potential; CYP3A4 inducers may decrease plasma pazopanib concentrations. 
Pazopanib should not be used if chronic use of strong CYP3A4 inducers cannot be avoided. 
Patients may not have received a strong CYP3A4 inducer within 12 days prior to cycle 1 day 1. 
CYP Substrates: Concomitant use of pazopanib with agents with narrow therapeutic windows 
that are metabolized by CYP3A4, CYP2D6, or CYP2C8 is not recommended.  

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Concomitant use of pazopanib and simvastatin increases the risk of ALT elevations and should 
be undertaken with caution and close monitoring.  
Table 14: 
Strong CYP3A4 Inducers and Inhibitorsa 
Inducers: 
 
*aInhibitors: 
dexamethasone 
 
Boceprevir 
Conivaptan 
phenytoin 
 
Indinavir 
Itraconazole 
carbamazepine 
 
Nelfinavir 
Ketoconazole 
rifampin 
 
Lopinavir/ritonavir 
Mibefradil 
rifabutin 
 
Saquinavir 
Nefazodone 
rifapentin 
 
Telaprevir 
Posaconazole 
phenobarbital 
 
Ritonavir 
Voriconazole 
St. John’s Wort 
 
Clarithromycin 
Telithromycin 
a Because the lists of these agents are constantly changing, it is important to regularly consult a comprehensive list 
such as the one located at http://medicine.iupui.edu/ clinpharm/ddis/. 
6.4. 
Treatment Compliance 
6.4.1. 
TRC105 Treatment Compliance 
All TRC105 infusions will occur at the trial site under the direct supervision of the treating 
physician or his or her designee.  
6.4.2. 
Pazopanib Treatment Compliance 
Patients will be asked to record the day and time of pazopanib home dosing on a TRACON 
supplied log to be reviewed by site personnel prior to initiation of each new cycle.  
6.5. 
Patient Enrollment 
Patients will be manually enrolled by TRACON Pharmaceuticals and assigned an eight digit 
patient number. This eight digit number will be used to identify patients throughout their 
participation in the trial.  A regulatory binder will be provided and will include detailed 
instructions for the manual enrollment process.  

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7. 
ASSESSMENT OF EFFICACY 
7.1. 
Radiological Tumor Assessment 
The primary efficacy endpoint for phase 2 is PFS, defined as time from screening to either first 
disease progression or death from any cause per RECIST version 1.1 [68]. Patients alive at the 
time of analysis will be censored at the date of last disease assessment.    
The primary efficacy assessment will be best overall response as defined in Section 7.1.2.  The 
determination of antitumor efficacy will be based on objective tumor assessments made by the 
Investigator according to RECIST version 1.1 [68].  Investigators will make treatment decisions 
based on these assessments.  All lesions will be classified as target or non-target lesions at the 
Screening visit.  Each lesion designation will be maintained through the course of the study.  
The same method and technique should be used to characterize each identified and reported 
lesion at Screening, during the study treatment period, and at the End of Study visit. Imaging-
based evaluation over clinical examination is the required technique when both could be used to 
assess the antitumor effect of the treatment.  Clinical Oncology review of all tumor 
measurements is desired. 
Whenever possible, clinical evaluation of superficial lesions should not be used as the sole form 
of measurement.  However, when necessary, color photograph with metric caliper is acceptable.  
Tumor evaluation by positron emission tomography (PET) scan or by ultrasound may not 
substitute for CT or MRI scans.  
Radiological tumor assessments will be performed at screening, as outlined in the Schedule of 
Assessments (Table 4,Table 5, Table 6 and Table 7), and whenever disease progression is 
suspected.  Another tumor assessment will be performed at the End of Study Visit if an 
assessment has not been performed within the prior 8 weeks.  All patient files and radiological 
images must be available for CRF source verification.     
7.1.1. 
Measurability of Tumor Lesions 
At Screening, individual tumor lesions will be categorized by the Investigator as either target or 
non-target according to RECIST 1.1 as described below. 
• Measurable:  Lesions that can be accurately measured in at least one dimension (longest 
diameter to be recorded) as ≥ 10 mm with spiral CT scan. Lytic bone lesions, with an 
identifiable soft tissue component, evaluated by CT or MRI, can be considered 
measurable lesions if the soft tissue component otherwise meets the definition of 
measurability previously described.  Blastic bone lesions are non-measurable. Lesions in 
previously irradiated areas (or areas treated with local therapy) should not be selected as 
target lesions, unless there has been demonstrated progression in the lesion. Clinical 
lesions will only be considered measurable when they are superficial (e.g. skin nodules, 
palpable lymph nodes) and ≥ 10 mm.  Clinical lesions must be measured with calipers. 
• Non-Measurable:  All other lesions, including small lesions and bone lesions other than 
lytic bone lesions, leptomeningeal disease, ascites, pleural or pericardial effusions, 
lymphangitis of the skin or lung, abdominal masses that are not confirmed and followed 
by imaging techniques, previously irradiated lesions (unless there has been demonstrated 

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progression in the lesion), and disease documented by indirect evidence only (e.g. by 
laboratory tests such as alkaline phosphatase).  
7.1.1.1. 
Recording Tumor Measurements 
Measurable lesions up to a maximum of 5 lesions representative of all involved organs (with a 
maximum of 2 lesions per organ) should be identified as target lesions and measured and 
recorded at Screening and at the stipulated intervals during treatment.  Target lesions should be 
selected on the basis of their size (lesion with the longest diameters) and their suitability for 
accurate repetitive measurements (either by imaging techniques or clinically).  Target lesions 
may include lymph nodes with a short axis > 15 mm.  
The longest diameter will be recorded for each target lesion (with the exception of lymph nodes, 
where the short axis will be used). The sum of the diameter for all target lesions at Screening will 
be calculated and recorded as the baseline sum diameter to be used as reference to further 
characterize the objective tumor response of the measurable dimension of the disease during 
treatment.  All measurements should be performed using a caliper or ruler and should be 
recorded in metric notation in millimeters. 
All other lesions (or sites of disease) should be identified as non-target lesions and should also be 
recorded at baseline.  Measurements are not required and these lesions should be followed as 
“present”, “stable”, “absent”, “increased” or “decreased”. 
7.1.2. 
Definitions of Tumor Response 
7.1.2.1. 
Target Lesions 
• Complete response (CR) is defined as the disappearance of all target lesions. 
• Partial response (PR) is defined as a ≥ 30% decrease in the sum of the dimensions of 
the target lesions taking as a reference the baseline sum dimensions. 
• Progressive disease (PD) is defined as a ≥ 20% relative increase and ≥ 5 mm absolute 
increase in the sum of the dimensions of the target lesions taking as a reference the 
smallest sum of the dimensions recorded since the treatment started, or the appearance of 
one or more new lesions. 
• Stable disease (SD) is defined as neither sufficient shrinkage to qualify for PR nor 
sufficient increase to qualify for PD taking as a reference the smallest sum of the 
dimensions since the treatment started.  
7.1.2.2. 
Non-Target Lesions 
• Complete response (CR) is defined as the disappearance of all non-target lesions. 
• non-CR/non-PD is defined as a persistence of ≥ 1 non-target lesions. 
• Progressive disease (PD) is defined as unequivocal progression of existing non-target 
lesions, or the appearance of ≥ 1 new lesions. 

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The cytological confirmation of the neoplastic origin of any effusion that appears or worsens 
during treatment when the measurable tumor has met criteria for response or stable disease is 
mandatory to differentiate between response or stable disease and progressive disease. 
7.1.2.3. 
Determination of Overall Response  
7.1.2.3.1. By RECIST  
When both target and non-target lesions are present, individual assessments will be recorded 
separately.  The overall assessment of response will involve all parameters as depicted in 
Table 15.  Per RECIST 1.1, in non-randomized trials with response as a primary endpoint, 
confirmation of PR or CR is required.  Per RECIST 1.1, a modest increase in the size of one or 
more non-target lesions is usually not sufficient to qualify for unequivocal progression status.  
Table 15: 
Response Evaluation Criteria in Solid Tumors 
Target Lesionsa 
Non-target Lesionsb 
New Lesionsc 
Overall Response 
CR 
CR 
No 
CR 
CR 
Non-CR/non-PD 
No 
PR 
CR 
Not evaluated 
No 
PR 
PR 
Non-PD or not all 
evaluated 
No 
PR 
SD 
Non-PD or not all 
evaluated 
No 
SD 
Not all evaluated 
Non-PD 
No 
Not Evaluable 
PD 
Any Response 
Yes or No 
PD 
Any Response 
PD 
Yes or No 
PD 
Any Response 
Any Response 
Yes 
PD 
aMeasurable lesions only. 
bMay include measurable lesions not followed as target lesions or non-measurable lesions. 
cMeasurable or nonmeasurable lesions.  
The best overall response is the best response recorded from the start of the treatment until 
disease progression/recurrence (taking as reference for progressive disease the smallest 
measurements recorded since the treatment started).  The patient’s best response assignment will 
depend on the achievement of both measurement and confirmation criteria. 
NOTE:  Patients with a global deterioration of health status requiring discontinuation of 
treatment without objective evidence of disease progression at that time should be reported as 
“Need for additional anti-cancer therapy/surgery”.  Every effort should be made to document the 
objective progression even after discontinuation of treatment.   
In some circumstances, it may be difficult to distinguish residual disease from normal tissue.  
When the evaluation of complete response depends upon this determination, it is recommended 
that the residual lesion be investigated by fine needle aspirate or biopsy before confirming the 
complete response status. 

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8. 
ASSESSMENT OF SAFETY 
8.1. 
Safety Parameters 
Safety will be characterized in terms of the incidence, timing, severity (graded by the National 
Cancer Institute [NCI] Common Terminology Criteria for Adverse Events [CTCAE], Version 
4.0), seriousness, and relatedness of adverse events and laboratory abnormalities.  In addition, 
physical examination, vital signs, and ECOG performance status will be serially monitored.  
Laboratory safety analyses will be based on the local laboratory data, and will include 
hematology, serum chemistry (including liver and kidney function), urinalysis, serum or urine 
pregnancy testing, and coagulation profile. Serum will also be assessed for immunogenicity to 
TRC105 (including anti-product antibody titers).  In addition, single tracing 12-lead ECGs will 
be performed at the time-points indicated in the Schedule of Assessments (Table 4, Table 5, 
Table 6 and Table 7).  QT, PR and QRS intervals and heart rate will be captured. ECGs will also 
be collected as clinically indicated throughout the study.   
8.1.1. 
Laboratory Safety Assessments 
Abnormal and clinically significant laboratory tests should be recorded as adverse events.  To 
meet the definition of clinically significant, the test result generally requires a change in medical 
management (e.g. new medication, unplanned treatment, additional tests, etc.). 
8.1.1.1. 
Hematology, Serum Chemistry, Coagulation, Pregnancy Test  
Assessments will be performed at the time points indicated in the Schedule of Assessments 
(Table 4, Table 5, Table 6 and Table 7) and analyzed at local laboratories.  Investigators may 
have additional blood tests performed for the purpose of planning treatment administration, or 
for following adverse events as clinically indicated.  
• Hematology: CBC with differential and platelet count. Iron studies (serum iron, ferritin 
and total iron binding capacity). 
• Coagulation: International Normalized Ratio (INR) will be assessed 
• Serum Chemistry: Total bilirubin, alanine transaminase (ALT), aspartate transaminase 
(AST), alkaline phosphatase, lipase, amylase, total protein, albumin, sodium, potassium, 
bicarbonate, chloride, calcium, phosphorus, blood urea nitrogen, creatinine, magnesium, 
thyroid stimulating hormone and glucose 
• Pregnancy Test: Serum or urine pregnancy tests will be performed locally on all female 
patients of childbearing potential. Patients must be surgically sterile (i.e.: hysterectomy) 
or be postmenopausal, or must agree to use effective contraception during the study and 
for 3 months following last dose of TRC105.  The definition of effective contraception 
will be based on the judgment of the Principal Investigator or a designated associate. 
8.1.1.2. 
Urinalysis  
Urinalysis (without microscopic analysis, unless indicated) will be performed at time points 
indicated in the Schedule of Assessments (Table 4, Table 5, Table 6 and Table 7) and analyzed 

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by local laboratories.  Microscopic analysis, urine protein-creatinine ratio (UPCR), and 24 urine 
collection for protein should be performed as clinically indicated. 
8.1.1.3. 
Physical Examination  
A physical examination including, but not limited to, general appearance, head, eyes, ears, nose, 
throat, neck, heart, chest, abdomen, musculoskeletal, extremities, skin, lymph nodes, 
neurological genitourinary (as appropriate), and rectal (as appropriate) will be assessed at 
timepoints indicated within the Schedule of Assessments (Table 4, Table 5, Table 6 and Table 7).  
The physical examination will include examination of known and suspected sites of disease.   
8.1.1.4. 
Vital Signs  
Heart rate, temperature, blood pressure, respiratory rate and weight will be assessed at time 
points indicated within the Schedule of Assessments (Table 4, Table 5, Table 6 and Table 7).  
Heart rate, temperature, blood pressure, and respiratory rate will also be assessed during TRC105 
infusions as described in Section 4.1.2.2 and the footnotes of Table 4, Table 5, Table 6 and 
Table 7(Schedule of Assessments).  
8.1.1.5. 
Performance Status  
The ECOG scale will be used to assess performance status at Screening.  
8.1.1.6. 
ECG  
A single 12-lead (with a 10–second rhythm strip) tracing will be used for all ECGs.  It is 
preferable that the machine used has a capacity to calculate standard intervals automatically. 
ECG will be performed at the time-points indicated in the Schedule of Assessments (Table 4, 
Table 5, Table 6 and Table 7) and as clinically indicated throughout the study.  
8.2. 
Adverse Events 
All observed or volunteered adverse events regardless of suspected causal relationship to 
TRC105 study drug will be reported as described below.  
8.2.1. 
Definition of Adverse Event 
An adverse event is any untoward medical occurrence in a trial patient who is administered a 
drug or biologic (medicinal product); the event may or may not have a causal relationship with 
the medicinal product.  Examples of adverse events include, but are not limited to the following: 
• Clinically significant symptoms and signs including:  
o Worsening of signs and symptoms of the malignancy under trial (disease 
progression without worsening of signs and symptoms assessed by measurement 
of malignant lesions on radiographs or other methods should not be reported as 
adverse events). 
o Signs and symptoms resulting from drug overdose, abuse, misuse, withdrawal, 
sensitivity, dependency, interaction or toxicity.   

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o All possibly related and unrelated illnesses, including the worsening of a 
preexisting illness.  
o Injury or accidents.  Note that if a medical condition is known to have caused the 
injury or accident (hip fracture from a fall secondary to dizziness), the medical 
condition (dizziness) and the outcome of the accident (hip fracture from a fall) 
should be reported as 2 separate adverse events.   
o Symptoms or signs resulting from exposure in utero. 
• Abnormalities in physiological testing or physical examination findings that require 
clinical intervention or further investigation (beyond ordering a repeat confirmatory test). 
• Laboratory abnormalities that meet any of the following (Note: merely repeating an 
abnormal test, in the absence of any of the below conditions, does not constitute an 
adverse event.  Any abnormal test result that is determined to be an error does not require 
reporting as an adverse event.): 
o Test result that is associated with accompanying symptoms 
o Test result that requires additional diagnostic testing or medical/surgical 
intervention 
o Test result that leads to a change in TRC105 study drug dosing outside of 
protocol-stipulated dose adjustments or discontinuation from the trial, significant 
additional concomitant drug treatment, or other therapy 
o Test result that is considered to be an adverse event by the Investigator or 
TRACON 
8.2.2. 
Serious Adverse Events 
An adverse event that meets one or more of the following criteria/outcomes is classified as 
serious: 
• Results in death 
• Is life-threatening (i.e., at immediate risk of death) 
• Requires in patient hospitalization or prolongation of existing hospitalization 
• Results in persistent or significant disability/incapacity  
• Results in congenital anomaly/birth defect 
• Other important medical events that may not result in death, be life-threatening, or 
require hospitalization may be considered serious when, based upon appropriate medical 
judgment, they may jeopardize the patient or may require medical or surgical intervention 
to prevent one of the outcomes listed above.  Examples of such events are intensive 
treatment in an emergency room for allergic bronchospasm; blood dyscrasias or 
convulsions that do not result in hospitalization; or the development of drug dependence 
or drug abuse.  

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Serious also includes any other event that the Investigator or sponsor judges to be serious, or 
which is defined as serious by the HRA in the country in which the event occurred.   
Progression of the malignancy under study (including signs and symptoms of progression) 
should not be reported as SAEs unless the outcome is fatal during the trial or within the safety 
reporting period. Hospitalizations due to signs and symptoms of disease progression should not 
be reported as SAEs.  If the malignancy has a fatal outcome during the trial or within the safety 
reporting period, then the event leading to death must be recorded as an adverse event and as an 
SAE with CTC grade 5.  
The onset date of an SAE is defined as the date on which the event initially met serious criteria 
(e.g., the date of admission to a hospital).  The end date is the date on which the event no longer 
met serious criteria (e.g., the date the patient was discharged from a hospital).  
8.2.2.1. 
Hospitalization 
Adverse events associated with in-patient hospitalization, or prolongation of an existing 
hospitalization, are considered serious.  Any initial admission, even if the duration is less than 24 
hours is considered serious. In addition, any transfer within the hospital to an acute/intensive care 
unit is considered serious (e.g., transfer from the psychiatric wing to a medical floor or transfer 
from a medical floor to a coronary care unit). However, the following hospitalizations should 
not be considered serious:  
• Rehabilitation facility admission 
• Hospice facility admission 
• Respite care 
• Skilled nursing facility admission 
• Nursing home admission 
• Emergency room visit 
• Outpatient same day surgery/procedure 
• Hospitalization or prolongation of hospitalization in the absence of precipitating clinical 
adverse events as follows: 
o Admission for treatment of preexisting condition not associated  with the 
development of a new adverse event or with a worsening of the preexisting 
condition  
o Social admission 
o Administrative admission (e.g. for yearly physical exam) 
o Protocol-specified admission during a clinical trial 
o Optional admission not associated with a precipitating clinical adverse event (e.g. 
for elective cosmetic surgery) 
o Preplanned treatments or surgical procedures that are not related to an SAE 

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o Hospitalization for observation without an AE 
• Diagnostic and therapeutic noninvasive and invasive procedures, such as surgery, should 
not be reported as adverse events.  The medical condition for which the procedure was 
performed should be reported if it meets the definition of an adverse event (e.g. acute 
appendicitis that begins during the adverse event reporting period should be reported as 
an adverse event and the appendectomy should be recorded as a concomitant treatment). 
8.3. 
Reporting Adverse Events 
8.3.1. 
Eliciting Adverse Event Information 
The Investigator is to report all directly observed adverse events and all adverse events 
spontaneously reported by the trial patient using concise medical terminology.  In addition, each 
trial patient will be questioned about adverse events at each clinic visit following initiation of 
treatment.  The question asked will be, “Since your last clinic visit have you had any health 
problems?” 
8.3.2. 
Adverse Event Reporting Period 
Safety information for each patient will be collected from the date of informed consent.  Adverse 
events occurring prior to the initiation of the study treatment with pazopanib or TRC105 study 
drug will be considered "baseline-signs and symptoms”, will be recorded on corresponding case 
report forms and will not be retained for patients who fail screening. The adverse event reporting 
period for this trial begins when the patient has received even a portion of the first dose of 
pazopanib or TRC105 study drug and ends 28 days after the last dose of pazopanib or TRC105 
study drug is administered.  
All adverse events that occur in trial patients during the adverse event reporting period specified 
in the protocol must be reported to TRACON, whether or not the event is considered study 
treatment-related.  In addition, any known untoward event that occurs beyond the adverse event 
reporting period that the Investigator assesses as possibly related to the investigational 
medication/product should also be reported as an adverse event. 
8.3.3. 
Reporting Requirements 
Each adverse event is to be classified by the Investigator as SERIOUS or NONSERIOUS.  This 
classification of the gravity of the event determines the reporting procedures to be followed.  If 
an SAE occurs, reporting will follow local and international regulations, as appropriate. 
The Investigator must notify the Sponsor of any event that meets one of the criteria for an SAE 
immediately upon learning of the event.  Any subsequent revisions that are made to information 
pertaining to serious suspected TRC105 adverse drug reactions (e.g.,: change in grade etc.), 
including a change in attribution to TRC105 study drug from “not related” to “suspected adverse 
drug reaction” should also be communicated to TRACON immediately. This notification should 
be made to: 
 
 

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PRIMARY MEDICAL MONITOR 
Charles Theuer, MD PhD 
TRACON Pharmaceuticals Inc. 
8910 University Center Lane, Suite 700 
San Diego, California 92122 
Email: ctheuer@traconpharma.com  
Cell Phone:  1.858.344.9400 
Office Phone:  1.858.550.0780 x233 
 
SECONDARY MEDICAL MONITOR 
Ron Shazer, MD MBA 
Email: rshazer@traconpharma.com  
Cell Phone:  1.310.922.8039 
 
 
Following notification, the Investigator will report the SAE via the AE CRF via the data 
management system.  The initial AE CRF is to be updated with more detailed adverse event 
information within 5 calendar days of the event. 
In the rare event that the Investigator is not immediately aware of an SAE (for example, if the 
study subject seeks urgent medical attention elsewhere), the Investigator is to notify the Sponsor 
immediately upon learning of it and document his/her first awareness. 
Each SAE should be followed until resolution, or until such time as the Investigator determines 
its cause or determines that it has become stable.  Information pertaining to follow-up of SAEs 
should also be sent to the TRACON Pharmaceuticals Inc. 
Serious adverse events that are unexpected and associated with use of the study medication will 
be reported to the US Food and Drug Administration (FDA) and all participating clinical sites by 
TRACON via MedWatch forms.  For events which are fatal or life-threatening, unexpected, and 
associated with use of the investigational product, a 7-Day Alert Report will be submitted to the 
FDA within 7 calendar days of receipt of the SAE information.  For all other events that are 
serious, unexpected, and associated with use of the investigational product, a written report will 
be made no more than 15 calendar days from the date TRACON learns of the event. 
Participating clinical sites will be notified of these events in parallel. 
All adverse events, including SAEs, are to be reported on the adverse event CRFs.  
8.3.4. 
Recording Adverse Events in the Case Report Forms 
The Investigator is to report all directly observed adverse events and all adverse events 
spontaneously reported by the trial patient.  In addition, each trial patient will be questioned 
about adverse events.  All adverse events that meet the criteria specified in Section 8.2.1 are to 

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be recorded on patient source documents and on the CRFs. Adverse events should be reported 
using concise medical terminology on the CRFs.  
8.3.5. 
Grading of Adverse Event Severity 
To report adverse events on the CRFs, the Investigator will use the severity grading as described 
in NCI CTCAE (Version 4.0).   
Every effort should be made by the Investigator to assess the adverse event according to CTCAE 
criteria. If the Investigator is unable to assess severity because the term is not described in NCI 
(Version 4.0), severity of MILD, MODERATE, SEVERE, LIFE-THREATENING, or FATAL 
may be used to describe the maximum intensity of the adverse event. For purposes of 
consistency, these intensity grades are defined as follows:  
Table 16: 
Adverse Event Grading 
Grade 
Non-CTCAE Severity 
Definition 
1 
Mild  
Does not interfere with patient’s usual function 
2 
Moderate 
Interferes to some extent with patient’s usual function 
3 
Severe 
Interferes significantly with patient’s usual function 
4 
Life-Threatening 
Results in immediate risk of patient’s death 
5 
Fatal 
Results in patient’s death 
Note the distinction between the severity and the seriousness of an adverse event.  A severe 
event is not necessarily a serious event.  For example, a headache may be severe (interferes 
significantly with patient’s usual function) but would not be classified as serious unless it met 
one of the criteria for serious events.     
8.3.6. 
Relationship to TRC105 Study Drug/Pazopanib 
In this study, TRC105 study drug is given in combination with Pazopanib.  The relationship of 
an adverse event to TRC105 study drug and/or Pazopanib should be classified by the Investigator 
using the following guidelines: 
• Suspected Adverse Reaction: There is a reasonable possibility that TRC105 caused the 
adverse event (i.e.: there is evidence to suggest a causal relationship between TRC105 
and adverse event). 
• Not Related: There is no reasonable possibility that the adverse event is associated with 
TRC105 study drug.  
AE’s related to TRC105 study drug or Pazopanib are considered Adverse Drug Reactions 
(ADR). 
8.3.7. 
Expectedness  
All TRC105 adverse events and adverse drug reactions are considered “unexpected” if it’s not 
listed in the investigator brochure or not listed at the specificity or severity that has been 
observed.  For example, under this definition, hepatic necrosis would be unexpected (by virtue of 

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greater severity) if the investigator brochure referred only to elevated hepatic enzymes or 
hepatitis.  Similarly, cerebral thromboembolism and cerebral vasculitis would be unexpected (by 
virtue of greater specificity) if the investigator brochure listed only cerebral vascular accidents.  
"Unexpected," as used in this definition, also refers to adverse events or suspected adverse 
reactions that are mentioned in the investigator brochure as occurring with a class of drugs or as 
anticipated from the pharmacological properties of the drug, but are not specifically mentioned 
as occurring with the particular drug under investigation. 
All Pazopanib adverse events and adverse drug reactions are considered “unexpected” if it’s not 
listed in the package insert or not listed at the specificity or severity that has been observed. 
"Unexpected," as used in this definition, also refers to adverse events or suspected adverse 
reactions that are mentioned in the package insert as occurring with a class of drugs or as 
anticipated from the pharmacological properties of the drug, but are not specifically mentioned 
as occurring with Pazopanib.  
8.3.8. 
Exposure in Utero 
If any trial patient (or partner of a trial patient) becomes or is found to be pregnant during the 
study or within 28 days of discontinuing the investigational medication/product, the Investigator 
must report the information to TRACON, or designee via the Pregnancy Notification Report 
Form.  This must be done irrespective of whether an adverse event has occurred and within 24 
hours of awareness of the pregnancy.  The information submitted should include the anticipated 
date of delivery.   
The Investigator will follow the patient (or partner of a trial patient) until completion of the 
pregnancy or until pregnancy termination (i.e., induced abortion) and then notify TRACON, or 
its designee, of the outcome within 5 days or as specified below.  The Investigator will provide 
this information as a follow-up to the initial report.  The reason(s) for an induced abortion must 
be specified. 
The Investigator should follow procedures for reporting an SAE if pregnancy outcome meets 
criteria for an SAE (i.e., spontaneous abortion, stillbirth, neonatal death, or congenital anomaly 
[including that in an aborted fetus]). 
In the case of a live birth, the “normality” of the newborn can be assessed at the time of birth and 
the Pregnancy Outcome Report Form should be completed (i.e., no minimum follow-up period 
of a presumably normal infant must pass before a Pregnancy Outcome Report Form can be 
completed).  The “normality” of an aborted fetus can be assessed by gross visual inspection 
unless pre-abortion laboratory findings are suggestive of a congenital anomaly. 
Additional information about pregnancy outcomes that are classified as SAEs follows:  
• “Spontaneous abortion” includes miscarriage and missed abortion. 
• All neonatal deaths that occur within 1 month of birth should be reported, without regard 
to causality, as SAEs.  In addition, any infant death after 1 month that the Investigator 
assesses as possibly related to the in utero exposure to the investigational medication 
should also be reported. 

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8.3.9. 
Follow-up of Unresolved Adverse Events  
All adverse events should be followed until they are resolved or the Investigator assesses them as 
chronic or stable.  Any increase or decrease in adverse event grade should be recorded as a new 
adverse event.  
All serious and those non-serious events assessed by the Investigator as possibly related to the 
investigational medication/product should continue to be followed even after the patient’s 
participation in the trial is over.  Such events should be followed until they resolve or until the 
Investigator assesses them as “chronic” or “stable.”  The event should also be documented on the 
adverse event CRF.  
8.4. 
Safety Monitoring 
The TRACON Clinical Team will monitor safety throughout the study via the following 
activities:  
• Surveillance for SAEs according to regulatory guidelines 
• Routine monitoring of non-serious adverse experiences as they are recorded in the case 
report forms and the source documents at study sites 
• A formally chartered TRACON in-house Safety Review Team that includes, among other 
staff, two physicians 
• A formally chartered external Safety Review Team that includes three physicians 
• Periodic teleconferences with the Principal Investigators to share experiences and ensure 
communication 
• Toxicity information that may affect the treatment of patients on this study will be 
promptly communicated in writing to all participating clinical sites and institutions 
participating in this clinical trial.      

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9. 
OTHER ASSESSMENTS 
9.1. 
Other Laboratory Assessments  
9.1.1. 
Pharmacokinetics  
Samples will be sent to Fisher BioServices (Franklin, MA 02038) for storage. See separate 
laboratory manual for specific collection, storage and shipping information.  
9.1.1.1. 
TRC105   Peak and Trough Concentration (Phase 1b dose levels -1, 1, 2 and 
expanded cohort 1 and angiosarcoma cohort 2) 
A 5 mL blood sample will be collected prior to dosing with TRC105 on the days indicated within 
the Schedule of Assessments (Table 4 and Table 7 ).   
A 5 mL blood sample will be collected within 10 minutes following each TRC105 infusion on 
the days indicated within the Schedule of Assessments (Table 4 and Table 7 ).   
Samples will be separated and stored at approximately -70 °C for shipment to third party 
laboratory. See separate laboratory guide for further collection and shipment information. 
9.1.1.2. 
TRC105 and Pazopanib Trough Concentration (Phase 1b dose levels 3, 
expanded cohort 2 and Phase 2 including angiosarcoma cohort 1) 
A 5 mL blood sample each for TRC105 and pazopanib pharmacokinetics to be collected at the 
time-points indicated in the Schedule of Assessments (Table 5, Table 6 and Table 7), prior to 
starting the TRC105 infusion and prior to pazopanib dosing.  Samples will be stored at 
approximately -70°C. Samples will be batch shipped as indicated in the laboratory manual to a 
third-party laboratory. See separate laboratory guide for further collection and shipment 
information. Additional PK samples may also be collected at the time of unexpected clinical 
events. 
9.1.2. 
TRC105 Immunogenicity 
Samples will be sent to Fisher BioServices (Franklin, MA 02038). See separate laboratory 
manual for specific collection, storage and shipping information. 
Anti-product antibody concentrations will be measured using validated ELISA methods at the 
time oints specified in the Schedule of Assessments ((Table 4, Table 5, Table 6 and Table 7) in 
all patients.  Anti-product antibody concentrations will be evaluated in the context of 
pharmacokinetic parameters and AE profiles. Samples will be separated and stored at 
approximately -70 °C for shipment to Fisher BioServices.  See separate laboratory guide for 
further collection and shipment information.  
9.1.3. 
Protein Biomarkers 
One 10mL purple top (K2EDTA) tube of blood will be collected on the days indicated within the 
Schedule of Assessments (Table 4, Table 5, Table 6 and Table 7).  Samples will be stored at 
approximately -70 °C and shipped to Fisher BioServices Inc. (10 Forge Park, Franklin, MA 
02038) for storage until the time of analysis. Duke University Medical Center will analyze 
plasma for several biomarkers including but not limited to VEGF, VEGF-R2, PIGF and sCD105 

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(Phase 1 Biomarker Laboratory, Duke University Medical Center, 309 MSRB, Research Dr., 
Durham, NC 27710).  Please see the separate laboratory guide for further collection and 
shipment information.  
9.1.4. 
Archival  Tumor Specimens 
Archival specimens (formalin-fixed, paraffin-embedded) of the primary cancer and/or metastatic 
cancer specimen for each study participant will be obtained.  It is preferable that the entire 
paraffin block be submitted, but if this is not feasible, then at least 20 unstained slides are 
requested for immunohistochemical analysis (sections of ~ 5 microns are preferred).  
Samples will be stored at room temperature and shipped to Fisher BioServices Inc. (10 Forge 
Park, Franklin, MA 02038) for storage until the time of analysis. See separate laboratory guide 
for further collection and shipment information. 
 

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10. 
STATISTICS 
10.1. 
Statistical Design/Sample Size 
10.1.1. 
Phase 1b Statistical Design/Sample Size 
The number of patients to be enrolled in this study will depend upon the observed safety profile, 
which will determine the number of patients per dose level and the number of dose escalations.  
It is anticipated that up to 30 patients will be enrolled in the phase 1b portion of the study. 
The probability of escalation to the next higher dose for each underlying true DLT rate is shown 
in Table 17.  For example, at a dose level with a true DLT rate of 5%, there is a greater than 95% 
probability of escalating.  Conversely, for a dose level with a true DLT rate of 70%, the 
probability of escalating is < 5%. 
Table 17: 
Probability of Escalation to the Next Dose for Each True Underlying DLT 
Rate at a Dose Level 
True Underlying DLT Rate 
5% 
10% 
20% 
30% 
40% 
50% 
60% 
70% 
80% 
90% 
Probability of Escalating Dose 
0.97 
0.91 
0.71 
0.49 
0.31 
0.17 
0.08 
0.03 
0.01 
0.001 
The probability of failing to observe DLT in a sample size of 3 or 6 patients given various true 
underlying DLT rates in shown in Table 18.  For example, with 6 patients, the probability of 
failing to observe DLT occurring at least 40% of the time is less than 5%. 
Table 18: 
Probability of Failing to Observe True Underlying DLT Rate at a Dose Level 
True Underlying DLT 
Rate 
5% 
10% 
20% 
30% 
40% 
50% 
60% 
70% 
80% 
90% 
Probability of Failing to 
Observe Toxicity if N = 
3 
0.86 
0.73 
0.51 
0.34 
0.22 
0.13 
.0064 
0.027 
0.008 
0.001 
Probability of Failing to 
Observe Toxicity if N = 
6 
0.74 
0.53 
0.26 
0.12 
0.047 
0.016 
0.0041 
<0.001 
<0.001 
<0.001 
 
The estimated MTD is the highest tested dose level with a DLT rate < 33% in at least 6 DLT 
evaluable patients. 
10.1.1.1. Definition of Analyzed Study Populations 
The following study populations will be considered when reporting study results: 
• The study population for safety includes all patients receiving at least a portion of 1 
dose of TRC105.   
• The study population for PK includes also subjects with adequate data for PK 
modeling of TRC105.   

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• The study population for efficacy will include all safety population patients who have 
baseline and follow-up tumor measurements as required for assessment by RECIST 
1.1. 
Patients who experience DLT who receive less than the prescribed dose of TRC105 or pazopanib 
due to documented toxicity in cycle 2 will be considered evaluable for dose escalation purposes. 
Only those patients who are deemed "ineligible" or who receive no therapy (i.e. no TRC105 or 
pazopanib) will be eliminated from the analysis.  Ineligible patients who receive therapy will not 
be included in the assessment of efficacy endpoints, but their data will be included in the 
assessment of all adverse event reporting.  
10.1.2. 
Phase 2 Statistical Design/Sample Size 
Sixty three patients will be treated in the phase 2 portion of the study. 
The study population for safety and efficacy will include all patients receiving at least a portion 
of one dose of TRC105.  
Assuming a true median PFS of 4.6 months, a 9-month accrual period, a 6-month follow-up 
period, and based on the use of a one-sided test at the alpha=0.05 level of significance, a sample 
size of 63 patients will provide 86% power to detect a 50% increase in the median PFS (i.e., a 
median of 6.9 months).  These power estimates are based on the approach of Lawless [1]. 
Two additional phase 2 cohorts of up to 13 patients each with angiosarcoma will be enrolled into 
separate cohorts, angiosarcoma cohort 1 and 2.  Angiosarcoma cohort 1: Assuming a 15% 
response rate by RECIST with pazopanib alone, 5 responders with TRC105 alone or in 
combination with pazopanib will provide >90% confidence the true response rate is >15% (two-
sided exact 90% binomial with 5 of 13 responders is 16.57 and 64.52%). Angiosarcoma cohort 2: 
Assuming a 15% response rate by RECIST with pazopanib alone, 5 responders with TRC105 in 
combination with pazopanib will provide >90% confidence that the true response rate is >15%.  
For each of the angiosarcoma cohorts, the two-sided exact 90% binomial with 5 of 13 responders 
is 16.57% and 64.52%. 
 
10.1.2.1. Definition of Analyzed Study Populations 
The following study populations will be considered when reporting study results for the phase 2 
portion: 
• The study population for safety includes all patients receiving at least a portion of 1 
dose of TRC105.   
• The study population for efficacy will include all safety population patients who have 
baseline and follow-up tumor measurements as required for assessment by RECIST 
1.1. 
Only those patients who are deemed "ineligible" or who receive no therapy (i.e., no TRC105 or 
pazopanib) will be eliminated from the analysis.  Ineligible patients who receive therapy will not 

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be included in the assessment of efficacy endpoints, but their data will be included in the 
assessment of all adverse event reporting.  
10.2. 
Data Analysis 
Descriptive statistics (such as means, medians, standard deviations and ranges for continuous 
data and percentages for categorical data) will be used to summarize patient characteristics, 
treatment administration/compliance, immunogenicity (anti-product antibodies), efficacy, 
pharmacokinetic parameters, protein biomarkers, and archival tumor tissue.  Data will also be 
displayed graphically, where appropriate. 
10.2.1. 
Analysis of Primary Objective  
10.2.1.1. Phase 1b Analysis of Primary Objective 
For each cohort, DLTs will be summarized by category (hematologic and non-hematologic) and 
by MedDRA preferred term.  
All AEs with an onset after initiation of treatment will be considered as treatment-emergent AEs.  
A preexisting condition that worsens during the treatment period will also be considered as a 
treatment emergent AE.  All AEs will be coded by system organ class (SOC) and preferred term 
using NCI CTCAE (MedDRA) version 4.0.  
The number and percentage of patients with the following types of treatment-emergent AEs will 
be summarized: common and serious AEs, AEs related to study medication, AEs resulting in 
study discontinuation, and clinically significant laboratory abnormalities. Non-treatment-
emergent serious AEs will be described separately.  Deaths will be reported with demographic 
information. 
10.2.1.2. Phase 2 Analysis of Primary Objective 
The primary analysis will test the null hypothesis that the median PFS is equal to 4.6 months.  
The time-to-event distribution for PFS will be estimated using the Kaplan-Meier method and the 
analysis will be based on the two-sided 90% confidence interval for the median (equivalent to 
the use of a one-sided test at the alpha=0.05 level of significance).   
Additional exploratory analyses of PFS will be conducted in subgroups defined based on number 
of prior therapies (one versus two or more), histology, and endoglin expression on sarcoma 
tissue.   
For the angiosarcoma cohorts, the overall response rate and median duration of response by 
RECIST 1.1 will be calculated and the response rate will include the two-sided 90% confidence 
interval. 
10.2.2. 
Analysis of Pharmacokinetics  
Peak and trough serum TRC105 concentrations will be measured using validated ELISA 
methods.   The TRC105 pharmacokinetic data will be assessed for potential correlations with 
response, PFS, survival, adverse events, and baseline characteristics using descriptive statistics 
and models as appropriate. 

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10.2.3. 
Objective Response 
The best response (CR, PR, SD or PD according to RECIST 1.1) for each patient with 
measurable disease who received at least one dose of TRC105 study drug will be listed by 
cohort.  Stable disease will be defined as lack of tumor progression lasting for 2 cycles or longer. 
10.2.4. 
Analysis of Protein Biomarkers 
Angiogenic protein biomarker data for each patient who received at least one dose of TRC105 
study drug will be listed. 
10.2.5. 
Analysis of Immunogenicity 
Anti-product antibody concentrations will be measured using validated ELISA methods at the 
timepoints specified in the Schedule of Assessments (Table 4, Table 5, Table 6 and Table 7).  
Anti-product antibody concentrations will be evaluated in the context of pharmacokinetic 
parameters and AE profiles. 
10.2.6. 
Analysis of Archival Tumor Tissue 
Endoglin expression within the tumor vasculature and on sarcoma tissue will be quantified for 
each patient who received at least one dose of TRC105 study drug and will be listed by 
histologic type of soft tissue sarcoma.  Expression will be determined by immunohistochemistry 
(IHC).  Other markers that may relate to efficacy or toxicity of TRC105 may also be explored. 
 

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11. 
DIRECT ACCESS TO SOURCE DATA/DOCUMENTS 
All data entered on CRFs/eCRFs must be verifiable within the patients’ source documents 
(written or electronic record).  The Investigator /institution guarantees TRACON representatives 
and appropriate regulatory authorities direct access to the original source records for the duration 
of the agreed study record retention period.  Printouts of source records that are electronically 
obtained and stored will not be acceptable for audit/inspection unless provided as certified exact 
copies and the data remains as meaningful and useful as in its original electronic state. 
Legally protected subject identification and other personal health information must be securely 
stored with limited access by the participating institutions. Unless secure provisions are 
established by the institution to allow TRACON (or designee) to perform remote monitoring of 
electronic source records, TRACON (or designee) will review source records/data on site and 
will not remove any such protected health information. 

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12. 
QUALITY CONTROL AND QUALITY ASSURANCE 
 Monitoring visits to clinical investigator sites will be made by TRACON or its representatives 
periodically during the trial to ensure that GCPs and all aspects of the protocol are being 
followed.   
The trial site will also be subject to possible inspection by the institutional review board (IRB) or 
independent ethics committee (IEC) or other appropriate regulatory authority.  The trial site is 
also subject to quality assurance audits performed by TRACON or its representatives. 
It is important that the Investigator(s) and their relevant personnel are available during the 
monitoring visits, audits, and inspections and that sufficient attention, time, and support is 
devoted to the process.  
TRACON and its representatives will be governed by applicable regulations, good clinical 
practice standards, and internal SOPs for the conduct of monitoring visits and QA audits. 
 

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13. 
ETHICS 
13.1. 
Institutional Review Board (IRB)/Independent Ethics Committee 
(IEC) 
It is the responsibility of the Investigator to have approval of the trial protocol, protocol 
amendments, informed consent forms, and advertisements from the IRB/IEC before potential 
patients are consented for participation on the trial. All correspondence and other evidence of 
appropriate and timely communications with the IRB/IEC should be retained in the 
Investigator/site files. Copies of all IRB/IEC approvals should also be forwarded to TRACON. 
The only circumstance in which an amendment may be initiated prior to IRB/IEC approval is 
where the change is necessary to eliminate apparent immediate hazards to the patients.  In that 
event, the Investigator must notify the IRB/IEC and TRACON in writing within 5 business days 
after the implementation. 
13.2. 
Ethical Conduct of the Study 
The trial will be performed in accordance with the protocol, applicable local regulatory 
requirements and laws, and the International Conference on Harmonization Guideline on Good 
Clinical Practice, which supports the application of ethical principles that have their origin in the 
Declaration of Helsinki (see ICH E6, § 2.1).  
13.3. 
Written Informed Consent 
The informed consent form language must be agreed upon by TRACON and the IRB/IEC and 
must be in compliance with ICH GCP, local regulatory requirements, and legal requirements. 
The informed consent information must not be changed without prior approval by TRACON and 
the IRB/IEC. The informed consent form used in this trial, and any changes made during the 
course of the trial, must be approved by both the IRB/IEC and TRACON, or designee, before 
use.  
It is the responsibility of the Investigator to give each patient full and adequate verbal and written 
information regarding the objective and procedures of the trial and the possible risks involved.  
This information must be provided to the patient prior to undertaking any trial-related procedure.  
Patients must be informed about their right to withdraw from the trial at any time.  Furthermore, 
it is the responsibility of the Investigator to ensure all patients are appropriately informed before 
obtaining their signed and dated consent.  Signatures from the investigator conducting the 
informed consent discussion should also be obtained, prior to undertaking any trial-related 
procedure. Consent by a legally authorized representative is not permitted. Should an impartial 
witness be needed, ICH E6 requirements for impartial witnesses will apply.  
The Investigator will retain the original of each patient’s signed consent form in the 
Investigator/site files. 
13.4. 
Patient Compensation 
Patients will not be compensated for participation in this trial; this will be outlined in the patient 
informed consent form. 

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14. 
DATA HANDLING AND RECORDKEEPING 
14.1. 
Inspection of Records 
CRF’s are required and should be completed for each patient who receives treatment with 
pazopanib or TRC105.  Screen failure CRF’s will not be collected.    Nevertheless, records of 
potential patients identified and screened shall be retained on site screening logs.  The completed 
original CRFs are the sole property of TRACON and should not be made available in any form 
to third parties without written permission from TRACON (except for authorized representatives 
of the HRA and in accordance with HIPAA regulations). 
It is the Investigator’s responsibility to ensure completion and to review and approve all CRF 
data.    The investigator will sign off on his/her data per patient.  These signatures serve to attest 
that the investigator has reviewed and approved the information contained on the case report 
forms and that the information is complete, accurate, and true.  At all times, the Investigator has 
final personal responsibility for the accuracy and authenticity of all clinical and laboratory data 
entered on the CRFs.    
The use of electronic CRFs (eCRFs) to capture study data using automated computerized data 
capture systems does not change the principles and requirements for collecting study data.  The 
investigator still retains final personal responsibility for eCRF data and any associated data 
pertaining to it (e.g. metadata including any record of change to the originally recorded data).  
The investigator’s signed approval of the eCRF data serves to attest that the electronic data and 
all of its associated metadata (including changes) has been reviewed and accepted as complete, 
accurate, and true for each patient in the study. 
14.2. 
Retention of Records 
To allow for appropriate evaluations and/or audits by regulatory authorities or TRACON, the 
Investigator agrees to keep records, including the identity of all participating patients (sufficient 
information to link records, CRFs and hospital records), all original signed informed consent 
forms, copies of all CRFs, source documents, and detailed records of treatment disposition.  The 
Investigator should retain these records according to local regulations or as specified in the 
Clinical Trial Agreement, whichever is longer.   
If the Investigator relocates, retires, or for any reason withdraws from the study, then TRACON 
should be prospectively notified.  The study records must be transferred to an acceptable 
designee, such as another Investigator or another institution.  The Investigator must inform 
TRACON of any such transfer of responsibilities and properly identify the person or institution 
assuming the responsibility.  The responsible investigator/institution must obtain TRACON’s 
written permission before disposing of any records.  

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15. 
DEFINITION OF END OF TRIAL 
15.1. 
End of Trial in all Participating Countries 
End of trial in all participating countries is defined as the time at which all patients enrolled in 
the study have completed treatment on study. 
15.2. 
End of Trial in a Member State 
End of trial in a Member State of the European Union is defined as the time at which it is 
deemed that sufficient patients have been recruited and completed the trial as stated in the 
regulatory application (e.g. the Clinical Trials Agreement (CTA)) and ethics application in the 
Member State.  Poor recruitment is not a reason for premature termination but is considered a 
normal conclusion to the trial in that Member State.  
15.3. 
TRACON Discontinuation Criteria 
Premature termination of this trial may occur because of a regulatory authority decision, change 
in opinion of the IRB/IEC, drug safety problems, or at the discretion of TRACON.  In addition, 
TRACON retains the right to discontinue development of TRC105 at any time.  
TRACON reserves the right to discontinue the trial prior to inclusion of the intended number of 
patients, but intends only to exercise this right for valid scientific or administrative reasons. If a 
trial is prematurely terminated or discontinued, TRACON will promptly notify the Investigator.  
After notification, the Investigator must contact all participating patients within a 28 day time 
period.  As directed by TRACON, all trial materials must be collected and all CRF data must be 
completed to the greatest extent possible.  

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16. 
PUBLICATION OF TRIAL RESULTS 
Publication of trial results is discussed in the Clinical Trial Agreement.  

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17. 
FINANCING AND INSURANCE 
Financing and Insurance are discussed in the Clinical Trial Agreement.  

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18. 
INVESTIGATOR PROTOCOL AGREEMENT: 105SAR101 
I understand that all information concerning this study supplied to me by TRACON 
Pharmaceuticals, Inc. is confidential information.  I have read this protocol and agree to conduct 
the study according to all applicable regulations, Good Clinical Practice Guidelines, and in 
accordance with the Clinical Trial Agreement. 
I understand that this protocol and all amendments must be submitted to the appropriate 
IRB/IEC. 
 
 
Investigator Name (PLEASE PRINT):  ______________________________________________ 
 
 
 
Signature: _________________________________________________Date: _______________ 
 
 
Please sign and return this agreement to: 
TRACON Pharmaceuticals, Inc.  
Attn: Clinical Operations  
8910 University Center Lane, Suite 700 
San Diego, CA  92122 
 
Please keep a copy for your records. 
 

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19. 
REFERENCES 
1. 
J., L., Statistical Models and Methods for Lifetime Data1982, New York John Wiley and 
Sons. 
2. 
Carmeliet, P., Angiogenesis in health and disease. Nat Med, 2003. 9(6): p. 653-60. 
3. 
Folkman, J. and Y. Shing, Angiogenesis. J Biol Chem, 1992. 267(16): p. 10931-4. 
4. 
Hurwitz, H., et al., Bevacizumab plus irinotecan, fluorouracil, and leucovorin for 
metastatic colorectal cancer. N Engl J Med, 2004. 350(23): p. 2335-42. 
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Sandler, A., et al., Paclitaxel-carboplatin alone or with bevacizumab for non-small-cell 
lung cancer. N Engl J Med, 2006. 355(24): p. 2542-50. 
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Miller, K.D., et al., Randomized phase III trial of capecitabine compared with 
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Friedman, H.S., et al., Bevacizumab alone and in combination with irinotecan in 
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Escudier, B., et al., Phase III trial of bevacizumab plus interferon alfa-2a in patients with 
metastatic renal cell carcinoma (AVOREN): final analysis of overall survival. J Clin 
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Escudier, B., et al., Sorafenib in advanced clear-cell renal-cell carcinoma. N Engl J Med, 
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Llovet, J.M., et al., Sorafenib in advanced hepatocellular carcinoma. N Engl J Med, 
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Motzer, R.J., et al., Sunitinib versus interferon alfa in metastatic renal-cell carcinoma. N 
Engl J Med, 2007. 356(2): p. 115-24. 
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Rini, B.I., Vascular endothelial growth factor-targeted therapy in metastatic renal cell 
carcinoma. Cancer, 2009. 115(10 Suppl): p. 2306-12. 
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Jemal, A., et al., Cancer statistics, 2010. CA Cancer J Clin, 2010. 60(5): p. 277-300. 
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Hogendoorn, P.C., et al., Changing concepts in the pathological basis of soft tissue and 
bone sarcoma treatment. Eur J Cancer, 2004. 40(11): p. 1644-54. 
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Kawaguchi, S., et al., A quest for therapeutic antigens in bone and soft tissue sarcoma. J 
Transl Med, 2005. 3: p. 31. 
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Dominici, M., et al., Minimal criteria for defining multipotent mesenchymal stromal 
cells. The International Society for Cellular Therapy position statement. Cytotherapy, 
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17. 
Tolar, J., et al., Sarcoma derived from cultured mesenchymal stem cells. Stem Cells, 
2007. 25(2): p. 371-9. 
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Postiglione, L., et al., Differential expression and cytoplasm/membrane distribution of 
endoglin (CD105) in human tumour cell lines: Implications in the modulation of cell 
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20. 
APPENDICES 
20.1. 
Appendix 1:  National Cancer Institute (NCI) Common Terminology 
Criteria for Adverse Events (CTCAE) 
The NCI CTCAE (Version 4.0) should be used to assess Adverse Events and may be reviewed 
on-line at the following NCI website: 
http://ctep.cancer.gov/protocolDevelopment/electronic_applications/docs/ctcaev4.pdf 
 
 

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20.2. 
Appendix 2:  ECOG Performance Status 
 
Grade 
Performance 
0 
Fully active, able to carry on all pre-disease performance without restriction. 
1 
Restricted in physically strenuous activity but ambulatory and able to carry out work of a light 
or sedentary nature, e.g., light house work, office work. 
2 
Ambulatory and capable of all self-care but unable to carry out any work activities. Up and 
about more than 50% of waking hours. 
3 
Capable of only limited self-care, confined to bed or chair more than 50% of waking hours. 
4 
Completely disabled. Cannot carry on any self-care. Totally confined to bed or chair. 
5 
Dead.