A Phase I/II Dose-Escalation and Expansion Study of the Selective PKC-β Inhibitor MS-553 in Patients With Chronic Lymphocytic Leukemia/Small Lymphocytic Lymphoma
Phase: PHASE1
Conditions: Chronic Lymphocytic Leukemia, Small Lymphocytic Lymphoma, Aggressive Lymphoma
Sponsor: MingSight Pharmaceuticals, Inc

Protocol MS-553-103 
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Clinical Protocol 
 
 
Study Title:  
A Phase I/II Dose-Escalation and Expansion Study of the Selective 
PKC-β Inhibitor MS-553 in Patients with Chronic Lymphocytic 
Leukemia/Small Lymphocytic Lymphoma 
Protocol Number:  
 
MS-553-103 
Investigational Drug:   
MS-553 
Sponsor:  
 
 
MingSight Pharmaceuticals Inc. 
 
 
 
 
 
San Diego, CA 92131 
 
Indication: 
 
 
Chronic Lymphocytic Leukemia and Small Lymphocytic Lymphoma 
 
Original Protocol Date: 
02 May 2017 
Protocol Version 2.1:  
25 October 2017 
Protocol Version 3.0:  
Amendment 1.0/ 20 February 2019 
Protocol Version 4.0:  
Amendment 2.0/ 28 August 2019 
Protocol Version 5.0:  
Amendment 3.0/ 31 January 2020 
Protocol Version 6.0:  
Amendment 4.0/ 07 April 2021 
Protocol Version 7.0:  
Amendment 5.0/ 29 August 2022 
Protocol Version 8.0:  
Amendment 6.0/ 06 June 2023 
Protocol Version 8.1  
Amendment 6.1/ 16 June 2023 
 

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Protocol Signature Page 
 
 
Protocol Title:  
A Phase I/II Dose-Escalation and Expansion Study of the Selective PKC-
β Inhibitor MS-553 in Patients with Chronic Lymphocytic 
Leukemia/Small Lymphocytic Lymphoma 
Protocol Number:  
MS-553-103 
 
 
This protocol has been approved by MingSight Pharmaceuticals, Inc. The following person 
is authorized on behalf of MingSight to approve this protocol and the signature below 
documents approval. 
 
 
 
 
 
 
 
 
 
 
 
Date 
 
MingSight Pharmaceuticals 
 

Protocol MS-553-103 
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INVESTIGATOR’S AGREEMENT 
 
Protocol Number: 
MS-553-103  
 
Protocol Version 8.1: Amendment 6.1 / 16 June 2023 
 
I have read this protocol in its entirety, and I agree to all aspects.  
• I agree to implement and conduct this study diligently and in strict compliance 
with the protocol, good clinical practices and all applicable laws and regulations. 
 
• I have read and agree to comply with the Investigator obligations stated in this 
protocol. Any changes in procedure will only be made if necessary to protect the 
safety, rights or welfare of subjects. 
 
• I have read and understand the MS-553 Investigator’s Brochure. 
 
• I agree to conduct in person or to directly supervise the trial. 
 
• I agree to ensure that all that assist me in the conduct of the study are aware of 
their obligations. 
 
• I agree to maintain all information supplied by MingSight Pharmaceuticals Inc. in 
confidence and, when this information is submitted to an Institutional Review 
Board (IRB), Independent Ethics Committee (IEC) or another group, it will be 
submitted with a designation that the material is confidential. 
 
Printed Name of Investigator 
 
Signature of Investigator 
 
Date 
 

Protocol MS-553-103 
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SYNOPSIS 
Name of Sponsor/Company: MingSight Pharmaceuticals, Inc. 
Name of Investigational Product: MS-553 
IND Number: 135339 
Title of Study:  A Phase I/II Dose-Escalation and Expansion Study of the Selective PKC-β 
Inhibitor MS-553 in Patients with Chronic Lymphocytic Leukemia/Small Lymphocytic 
Lymphoma 
Phase: I/II  
Comparator: None  
Number of Subjects:  Approximately 153 
Study Sites:  Up to approximately 15 sites 
Study Design: 
Single agent MS-553 cohorts (A-series): 
This is a Phase I/II multi-center, open-label study of MS-553 in patients with chronic 
lymphocytic leukemia (CLL)/small lymphocytic leukemia (SLL). The first part of the study, the 
dose escalation portion (Cohort A1), will determine the safety of MS-553 and the maximum 
tolerated dose (MTD) or initial recommended Phase 2 dose (RP2D) of MS-553 monotherapy. 
After the MTD or initial RP2D is identified, an expansion cohort (“Expansion Cohort A2”) of 
approximately 25 patients with refractory or relapsed CLL/SLL who (a) have failed at least two 
prior lines of therapies, or (b) have either acquired clinical and/or molecular resistance to a 
BTK inhibitor (i.e., BTK and/or PLCG2 mutation) after achieving initial response to a BTK 
inhibitor will be enrolled. 
In addition, an optional expansion cohort (“Expansion Cohort A3”) of approximately 25 
patients with aggressive lymphoma, including Richter’s transformation, diffuse large B-cell 
lymphoma (DLBCL) and mantle cell lymphoma (MCL) may be enrolled. At least 15 patients in 
this cohort shall be MCL patients. 
 
 
 
additional patients will be enrolled in Cohort A2 to allow testing of two dose levels before a 
final RP2D can be declared. 
The two dose schedules, designated as dose schedule 1 (DS1) and dose schedule 2 (DS2), 
are as follows: 

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Dose Schedule 1 (DS1) 
 
MS-553 Oral Daily Dose 
Week 1 
150 mg BID 
Week 2 
200 mg BID 
Week 3 and beyond 
250 mg BID 
 
Dose Schedule 2 (DS2) 
 
MS-553 Oral Daily Dose 
Cycle 1 and beyond 
225 mg BID 
 
Twenty of the 40 additional patients will be treated with DS1 and the other 20 patients with 
DS2. The patients will be allocated to either dose schedule in alternate fashion according to 
the sequence of their enrollment. For example, the first enrolled patient will be allocated to 
DS1, the second patient to DS2, the third patient to DS1, the fourth to DS2, and so on. 
The optional expansion cohort (Cohort A3) will be closed to accommodate the new dose 
testing plan. 
Combination Cohorts 
 
 
 
 
Combination Cohorts with acalabrutinib (B-series)  
As soon as the MTD or initial RP2D is declared for MS-553 monotherapy in Cohort A1, MS-
553 will be evaluated in combination with acalabrutinib.  
Cohort B1.The first part of the study will be a determination of MTD or initial RP2D of MS-
553 when combined with acalabrutinib in treatment experienced CLL/SLL patients who are 
BTK inhibitor naïve, eligible for standard of care treatment with a BTK inhibitor as a single 
agent and who have had no more than 2 previous lines of treament regimens. The number of 
patients in the dose escalation will range from 3 to approximately 12 patients, and start with 
a MS-553 dose of 150 mg BID in the first dose level. Once the MTD or initial RP2D of MS-553 
is known for the combination, two expansion cohorts (B2 and B3) will be started to further 
evaluate the safety of the combination regimens, as well as to assess early evidence of anti-
tumor activity as described below.   
Cohort B2. Once the MTD/ intial RP2D is established in Cohort B1 for MS-553 in combination 
with acalbrutinib, an expansion cohort will be opened. This cohort will evaluate MS-553 in 
combination with acalabrutinib in treatment experienced CLL/SLL patients who are BTK 
inhibitor naïve, are eligible for treatment with a BTK inhibitor as a single agent, and who have 
had no more than 2 previous lines of treatment regimens.  Approximately 20 patients will be 

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enrolled in Cohort B2 to further confirm the safety of the combination and assess early 
evidence of anti-tumor activity.  
Cohort B3. Once the MTD/initial RP2D is established in Cohort B1, Cohort B3 will be opened 
in parallel to Cohort B2 to evaluate MS-553 in combination with acalabrutinib in treatment 
experienced CLL/SLL patients who are BTKi naïve, have received no more than 2 prior lines of 
therapies and  have TP53 abnormalities (including 17p deletion and P53 mutations). Once 
this cohort is open, all patients with TP53 abnormalities who are also naïve to BTK inhibitors 
will be preferentially enrolled in Cohort B3, rather than Expansion Cohorts B2. Approximately 
10 treatment experienced CLL/SLL patients, eligible for standard of care acalabrutinib will be 
enrolled in Cohort B3 to further confirm the safety of the combination and assess early 
evidence of anti-tumor activity. 
Combination Cohorts with venetoclax and rituximab (C-series) 
As soon as the MTD or initial RP2D is declared for MS-553 monotherapy in Cohort A1, MS-
553 will be evaluated in combination with venetoclax and rituximab.  
Cohort C1. The first part of the study will be a determination of the MTD or initial RP2D of 
MS-553 in combination with venetoclax and rituximab in treatment experienced CLL/SLL 
patients eligible for standard of care venetoclax and rituximab, who are naïve to prior Bcl-2 
inhibitor treatment and who have had no more than 2 prior lines of treatment regimens. The 
number of patients in dose escalation will range from 3 to approximately 12 patients, and 
start with a MS-553 dose of 150 mg BID.  
Cohort C2. Once the MTD or initial RP2D of MS-553 is known for the combination of 
venetoclax, rituximab, and MS-553, an expansion cohort will be initiated.  Treatment 
experienced CLL/SLL patients who are eligible for standard of care venetoclax and rituximab, 
who are naïve to prior Bcl-2 inhibitor treatment and have had no more than 2 previous lines 
of treatment regimens can be enrolled. Approximately 20 patients will be enrolled to further 
confirm the safety of the combination and assess early evidence of anti-tumor activity. 
Objectives: 
Series A Cohorts 
Primary 
• Evaluate the safety of MS-553 monotherapy in patients with chronic lymphocytic 
leukemia (CLL)/small lymphocytic leukemia (SLL) whose disease relapsed after or was 
refractory to at least two prior lines of therapies with the following exceptions: 
• Patients with a 17p deletion who have already received venetoclax, or 
• Patients who have a TP53 mutation (prior therapy is not required for these 
patients), or 
• Patients who have either acquired clinical and/or molecular (e.g., BTK or 
PLCG2 mutation) resistance to a BTK inhibitor. 

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• Determine the MTD or the initial RP2D of MS-553 monotherapy. 
Secondary 
• Evaluate the clinical activity of MS-553 in patients with relapsed or refractory CLL/SLL 
who: 
• Have failed at least two prior lines of therapies, or 
• Have either acquired clinical and/or molecular (e.g., BTK or PLCG2 mutation) 
resistance to a BTK inhibitor.  
• 
Evaluate pharmacokinetics in patients receiving MS-553. 
Pursuit of the secondary objectives should not jeopardize the primary objective, which 
is safety. 
Exploratory 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Series B Cohorts 
Primary 
• Evaluate the safety of MS-553 in combination with acalabrutinib in patients with 
CLL/SLL. 
• Determine the MTD or the initial RP2D of MS-553 in combination with acalabrutinib.  
Secondary 
• Evaluate the clinical activity of MS-553 in combination with acalabrutinib in patients 
with CLL/SLL. 
• Evaluate pharmacokinetics in patients receiving MS-553 and acalabrutinib. 
Pursuit of the secondary objectives should not jeopardize the primary objective, which is 
safety. 
Exploratory 

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Series C Cohorts 
Primary 
• Evaluate the safety of MS-553 in combination with venetoclax and rituximab in 
treatment experienced CLL/SLL. 
• Determine the MTD or the initial RP2D of MS-553 in combination with venetoclax and 
rituximab.  
Secondary 
• Evaluate the clinical activity of MS-553 in combination with venetoclax and rituximab 
in patients with CLL/SLL.  
• Evaluate pharmacokinetics in patients receiving MS-553 in combination with 
venetoclax and rituximab. 
Pursuit of the secondary objectives should not jeopardize the primary objective, which is 
safety. 
Exploratory 
 
 
 
 
 
Endpoints: 
Primary Endpoints 
• Safety 
• Incidence of dose-limiting toxicities and treatment-emergent adverse events 
(TEAE) requiring study drug discontinuation. Assessments for DLT and TEAE will 
occur during Cycle 1 for Cohort A1, and the protocol defined DLT evaluation 
periods for Cohort B1 and Cohort C1 (See Section 0 and 3.3.4). TEAE will be 
assessed throughout the course of the trial. The primary endpoint will be the 
rate of DLT and TEAE requiring study drug discontinuation in the respective 
DLT evaluation periods of Cohort A1, Cohort B1, and Cohort C1. 
• MTD or initial RP2D as monotherapy, as well as in combination with either 
acalabrutinib or venetoclax and rituximab.  
Secondary Endpoints 
• Objective response rate (ORR), with overall response defined as best response of 
complete response (CR), CR with incomplete marrow recovery (CRi), partial response 

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(PR), or PR with lymphocytosis (PRwL) according to the 2008 International Workshop 
on Chronic Lymphocytic Leukemia (IWCLL) Response Criteria, with modifications for 
treatment-related lymphocytosis. 
• Duration of response (DoR), defined as time from first documentation of response to 
first documentation of progressive disease (PD) per 2008 IWCLL, first new therapy 
including allogeneic transplantation, or death due to any cause. 
• Progression free survival (PFS), defined as time from first dose of study treatment to 
the first documentation of PD, first new therapy (excluding allogeneic 
transplantation), or death due to any cause. 
• Overall survival (OS), defined as time from first dose of study treatment to death due 
to any cause. 
• Disease control rate (DCR) defined as the percentage of patients who have achieved 
CR, Cri, PR, PRwL and stable disease (SD). Duration of stable disease, with stable 
disease defined as at least 6 months from the start of MS-553 monotherapy to first 
documentation of progressive disease (PD) in relapsed and refractory CLL/SLL 
patients. 
• Pharmacokinetics of MS-553 monotherapy, as well as the small molecule agents used 
in combination, acalabrutinib, or venetoclax. 
Overview of Exploratory Endpoints 
• Serum plasma, blood, and tissue biomarker assays will be used to assess: 
 
 
 
 
 
 
 
 
 
 
 
 
  
• 
 
 
 
 
See Section 2.6 for a more detailed discussion of samples and endpoints by Cohort. 
Study Design and Methodology:  
This is a Phase I/II, multi-center, open-label study of single agent MS-553, as well as MS-553 in 
combination with acalabrutinib or venetoclax and rituximab in treatment experienced CLL or 
SLL patients.  

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The study will consist of 3 cohorts. Cohort A will evaluate MS-553 monotherapy, Cohort B 
will evaluate MS-553 in combination with acalabrutinib and Cohort C will evaluate MS-553 in 
combination with venetoclax and rituximab. 
Cohort A 
Patients will be treated in 28-day cycles. Patients who discontinue treatment for 
progressive disease and/or use of other CLL/SLL therapy will be followed for survival every 
24 weeks (± 7 days) for up to 3 years. Patients who discontinue treatment for reasons 
other than progressive disease, use of other CLL/SLL therapy, death, or withdrawal of 
consent will have response assessments every 12 weeks (± 7 days) until progressive 
disease occurs. Thereafter they will be followed for survival every 24 weeks (± 7 days) for 
up to 3 years.  All patients will discontinue survival follow-up upon completion of 3 years of 
survival follow-up, withdrawal of consent, death, or study closure, whichever is the 
earliest. 
Cohort A1 will follow a conventional “3+3” patient enrollment scheme for the dose 
escalation part of the study. Patients will be dosed with MS-553 by mouth twice daily at 
an initial dose (dose level 0, DL 0) of 100 mg BID daily in 28-day cycles. MS-553 shall be 
taken with food.  
Dose Level 
Dose 
0 
100 mg BID 
1 
200 mg BID 
2 
250 mg BID 
3 
300 mg BID 
4+ 
TBD* mg BID 
*To be determined 
After completion of dose level 3 (DL 3), the dose escalation of MS-553 can continue in 50 
mg BID increments (or lower if an intermediate dose is introduced) until a MTD is reached. 
Or, the Safety Review Committee and/or the Sponsor may declare an initial RP2D, if 
further dose escalation is not warranted prior to reaching MTD. Furthermore, in order to 
facilitate initial RP2D determination, select cohort(s) may be expanded up to 10 patients 
total in a cohort to assess the safety and clinical activity of MS-553 provided that the dose 
does not exceed the MTD and the Safety Review Committee, upon reviewing the safety 
and tolerability, efficacy, and available PK and PD data, agrees.    
During the dose escalation period, intra-patient dose escalation will be permitted at the 
discretion of the study investigators and after discussion with the medical monitor and 
Sponsor, if the following criteria are met: 
1. The next higher dose for the regimen has been demonstrated to be safe by at 
least 3 patients and all 3 patients complete one cycle without experiencing any 
dose limiting toxicity (DLT). 

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2. The patient who will receive the higher dose has not experienced a DLT and has 
also completed at least 2 cycles of therapy at the current dose. 
3. Intra-patient dose escalation can be increased by only one dose level at a time.  
After the MTD or initial RP2D is identified, an expansion cohort (“Expansion Cohort A2”) of 
approximately 25 patients with refractory or relapsed CLL/SLL who (a) have failed at least 
two prior lines of therapies, or (b) have acquired clinical and/or molecular resistance of a 
BTK inhibitor (i.e., BTK and/or PLCG2 mutation) after achieving initial response to a BTK 
inhibitor will be activated. 
In addition, an optional expansion cohort (Cohort A3) of approximately 25 patients with 
aggressive lymphoma, including Richter’s transformation, DLBCL and MCL, can be activated 
simultaneously with the activation of the two expansion cohorts. At least 15 patients in this 
cohort shall be MCL patients.  
Patients in the expansion cohorts will be treated at the MTD (or initial RP2D). 
All patients will continue to be monitored for approximately 30 days ( +7days)  after 
treatment discontinuation for development of any late treatment emergent adverse 
events. Any such late adverse events will be considered in the determination of the 
maximum tolerated dose/initial RP2D and dose schedule to be taken forward into the 
expansion phase of the study.  
 
 
 
additional patients will be enrolled in Cohort A2 to further testing two dose levels before a 
final RP2D can be declared. 
The two dose schedules designated as dose schedule 1 (DS1) and dose schedule 2 (DS2) are 
as follows: 
Dose Schedule 1 (DS1) 
 
MS-553 Oral Daily Dose 
Week 1 
150 mg BID 
Week 2 
200 mg BID 
Week 3 and beyond 
250 mg BID 
 
Dose Schedule 2 (DS2) 
 
MS-553 Oral Daily Dose 
Cycle 1 and beyond  
225 mg BID 
 
Twenty of the 40 additional patients will be treated with DS1 and the other 20 patients with 
DS2. The patients will be allocated to either dose schedule in alternate fashion according to 

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the sequence of their enrollment. For example, the first enrolled patient will be allocated to 
DS1, the second patient to DS2, the third patient to DS1, the fourth to DS2, and so on. 
The optional expansion cohort (Cohort A3) will be closed to accommodate the new dose 
testing plan. 
 
 
 
 
Cohort B 
Cohort B1. Once the MTD or initial RP2D is established in A1, Cohort B1 will be initiated. 
A conventional “3+3” patient enrollment scheme will be followed during this dose 
escalation Cohort B1. The dose escalation will enroll from 3 to approximately 12 patients. 
The initial MS-553 dose will be 150 mg BID. Treatment experienced CLL/SLL patients who 
are BTK inhibitor naïve, eligible for standard of care treatment of a BTK inhibitor as a 
single agent, and who have had no more than 2 previous lines of treatment regimens, 
will be enrolled for combination treatment with MS-553 and acalabrutinib.  Patients will 
be dosed with MS-553 by mouth twice daily and acalabrutinib as per the Prescribing 
Information. 
At least two dose levels are planned for Cohort B1. The dosing schedule and DLT evaluation 
periods are described in the table below. 
MS-553 Dose Levels 
Dosing Schedule 
DLT Evaluation Period 
150 mg BID 
In 28-day cycles 
Cycle 1 
200 mg BID 
In 28-day cycles 
Cycle 1 
MTD determination, DLT and safety assessments will follow the general principles 
outlined in Cohort A1.  Initial RP2D of MS-553 for the combination is expected to be 
reached before the dose escalation reaches 250 mg BID, the high dose level of MS-553 
under testing as a single agent therapy. Intermediate dose levels (i.e., at 25 mg 
increment) or lower dose levels may also be evaluated as determined by the 
Investigators, the Safety Review Committee and Sponsor until the MTD or initial RP2D for 
the combination is determined.  
Cohort B2. After the MTD or initial RP2D is identified in Cohort B1, an expansion cohort of 
approximately 20 treatment experienced CLL/SLL patients who are BTK inhibitor naïve, 
eligible for standard of care treatment of a BTK inhibitor as a single agent, and who have 
had no more than 2 previous lines of treatment regimens will be enrolled at the initial 
RP2D.  
Cohort B3. In parallel with Cohort B2, an expansion cohort with treatment experienced 
CLL/SLL patients with TP53 abnormalities (including 17p deletion and P53 mutations), who 
are eligible for standard of care acalabrutinib will be enrolled. Approximately 10 patients 
will be enrolled for dosing with MS-553 and acalabrutinib to further confirm the safety of 

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the combination and assess early evidence of anti-tumor activity. Once this cohort is open, 
patients with TP53 abnormalities will be preferentially enrolled in Cohort B3, rather than 
Expansion Cohort B2. 
Patients in Cohort B1, B2, and B3. Patients will be treated in 28-day cycles until the 
combination is no longer tolerated or progressive disease. All patients will continue to be 
monitored for approximately 30 days ( +7days)  after treatment discontinuation for 
development of any late treatment emergent adverse events. Any adverse events noted 
only in patients enrolled in Cohort B1 within the DLT evaluation period will be considered in the 
determination of the maximum tolerated dose/initial RP2D and dose schedule to be taken 
forward into the expansion phase of the study.  
Patients who discontinue combination treatment for progressive disease and/or use of other 
CLL/SLL therapy will be followed for survival every 24 weeks (± 7 days) for up to 3 years. 
Patients who discontinue treatment for reasons other than progressive disease, use of other 
CLL/SLL therapy, death, or withdrawal of consent will have response assessments every 12 
weeks (± 7 days) until progressive disease occurs. Thereafter they will be followed for 
survival every 24 weeks (± 7 days) for up to 3 years.  All patients will discontinue survival 
follow-up upon completion of 3 years of survival follow-up, withdrawal of consent, death, or 
study closure, whichever is the earliest. 
Cohort C 
Cohort C1. Once the MTD or initial RP2D is established in A1, Cohort C1 will be initiated. 
A conventional “3+3” patient enrollment scheme will be followed during this dose 
escalation Cohort C1. The dose escalation will enroll from 3 to approximately 12 patients. 
The initial MS-553 dose will be 150 mg BID. Treatment experienced CLL/SLL patients 
eligible for standard of care venetoclax and rituximab, who are naïve to prior Bcl-2 
inhibitor treatment and who have had no more than 2 prior lines of treatment regimens 
are eligible for this cohort. Starting from Cycle 1, patients will be dosed with MS-553 by 
mouth twice daily and rituximab according to standard of care, and as outlined in Section 
5.2.3.3. Beginning with Cycle 3, venetoclax dosing once daily by mouth will be initiated 
with weekly dose escalations from 20 mg to 400 mg over 5 weeks per its prescribing 
information.  
At least two dose levels are planned for Cohort C1. The dosing schedule and DLT evaluation 
periods are described in the table below. 
Dose 
level 
Cycle 1 
Cycle 2 
Cycle 3 
Cycle 4+ 
DLT 
Period(s) 
150 mg 
BID 
150 mg 
BID 
150 mg 
BID 
150 mg 
BID 
150 mg 
BID 
Cycle 1 
through 4 
200 mg 
BID 
200 mg 
BID 
200 mg 
BID 
200 mg 
BID 
200 mg 
BID 
Cycle 1 
through 4 
 

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Initial RP2D of MS-553 for the combination is expected to be reached before the dose 
escalation reaches 250 mg BID, the high dose level of MS-553 under testing as 
monotherapy. Intermediate dose levels (i.e. at 25 mg increment) or even lower dose 
levels may also be evaluated as determined by the Investigators, the Safety Review 
Committee and Sponsor until the MTD or initial RP2D for the combination is determined.  
Cohort C2. After the MTD or initial RP2D is identified for the combination of MS-553, 
venetoclax and rituximab, an expansion cohort of approximately 20 patients will be 
initiated.  Treatment experienced CLL/SLL patients, eligible for standard of care venetoclax 
and rituximab, who are naïve to prior Bcl-2 inhibitor treatment and who have had no more 
than 2 prior lines of treatment regimens are eligible for this cohort to further confirm the 
safety of the combination and assess early evidence of anti-tumor activity. Venetoclax will be 
initiated on Cycle 3 following the administration of MS-553 and rituximab combination for 
two cycles. For this reason, the DLT evaluation period for the triplet combination is 
extended to 4 cycles of therapy in order to include ramping up of the venetoclax dose that 
is introduced in Cycle 3. 
Patients in Cohort C1 and C2 
Patients will be treated in 28-day cycles provided they continue to tolerate the drug, have 
no evidence of progression, and do not meet any of the criteria for discontinuation. All 
patients will continue to be monitored for approximately 30 days ( +7days)  after 
treatment discontinuation for development of any late treatment emergent adverse 
events. Any adverse events noted during the DLT evaluation periods will be considered in the 
determination of the maximum tolerated dose/initial RP2D and dose schedule to be taken 
forward into the expansion phase of the study.  
Patients who discontinue treatment for progressive disease and/or use of other CLL/SLL 
therapy will be followed for survival every 24 weeks (± 7 days) for up to 3 years. Patients 
who discontinue treatment for reasons other than progressive disease, use of other 
CLL/SLL therapy, death, or withdrawal of consent will have response assessments every 12 
weeks (± 7 days) until progressive disease occurs. Thereafter they will be followed for 
survival every 24 weeks (± 7 days) for up to 3 years.  All patients will discontinue survival 
follow-up upon completion of 3 years of survival follow-up, withdrawal of consent, death, 
or study closure, whichever is the earliest. 
Dose limiting toxicity, Dose Adjustments and Management of DLTs  
Cohort A1  
At least 3 patients will be enrolled at starting dose level 0 (100 mg BID for Cohort A1, 
MTD/initial RP2D level -1 for Cohort B1). During the first cycle of any dose level, if <1 DLT is 
observed, then escalation to the next higher dose level will follow the 3+3 design and 
proceed when 3/3 or 5/6 patients have completed one 28-day treatment cycle without a 
DLT. If at least 1 of 3 patients experiences a DLT at any dose level, the cohort will be 
expanded to 6 patients. If ≥ 2/3 or ≥ 2/6 patients experience a DLT within a cohort, the MTD 
will be considered to have been exceeded. No additional patients will be enrolled in that 
cohort, and dose escalation will stop. If at least 3/3 or 5/6 patients have completed one 28-

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day treatment cycle at the previous dose level without a DLT, then that dose may be 
accepted as the MTD. At Sponsor discretion, additional patients may be enrolled into a 
cohort where < 1 patient develops a DLT to obtain additional safety, PK, PD, and/or efficacy 
data.  
If greater than 1/6 patients experience a DLT at DL 1, then 100 mg BID may be declared the 
MTD, or intermediate dose levels and/or alternative dosing schedules may be evaluated 
upon review of safety, pharmacodynamic, and/or pharmacokinetic data.  
Cohort B1 
In Cohort B1, at least 3 patients will be enrolled at starting dose level of 150 mg BID.  The 
general principles of dose escalation and DLT evaluation rules described in Cohort A1 are 
applicable to Cohort B1.  
Cohort C1 
In Cohort C1, at least 3 patients will be enrolled at starting dose level of 150 mg BID.  The 
dosing rules for Cohorts A1 and B1 will be similarly applied to Cohort C1. For the dose levels 
of MS-553 up to and including 225 mg BID, the DLT evaluation period will be through Cycle 4 
(16 weeks). During the DLT evaluation period, if < 1 DLT is observed, then escalation to the 
next higher dose level will follow the 3+3 design and proceed when 3/3 or 5/6 patients have 
completed four treatment cycles without a DLT. If at least 1 of 3 patients experiences a DLT 
at any dose level, the cohort will be expanded to 6 patients. If ≥2/3 or ≥2/6 patients 
experience a DLT within a cohort, the MTD will be considered to have been exceeded. At 
Sponsor discretion, additional patients may be enrolled into a cohort where < 1 patient 
develops a DLT to obtain additional safety, PK, PD, and/or efficacy data.   
Determination of DLT for dosing Cohorts A1 
Each dose cohort will include a minimum of 3 evaluable patients for assessment of DLT. In 
order to be considered evaluable for DLT, patients must meet at least one of the following 
criteria:  
1. Complete one 28-day cycle and receive at least 75% of MS-553 intended doses in 
Cycle 1 
2. Discontinue due to DLT  
During the DLT evaluation period for each cohort, non-evaluable patients who discontinue 
treatment before one cycle is complete for reasons other than study drug-related toxicities 
(e.g., progressive disease, non-compliance), or do not receive at least 75% of MS-553 doses, 
will be replaced with additional patients enrolled at the same dose level. After a DLT is 
resolved or reversed to  Grade 1, the study drug may continue at a lower dose level at the 
discretion of the investigator. 
A decision on dose escalation to the next dose level will be based on evaluation of the 
clinical safety profile and may also include evaluation of MS-553 exposure in treated 
patients. After the completion of each cohort, safety and PK data (if available) will be 

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reviewed and a decision on dose escalation will be determined by the Investigators, the 
Safety Review Committee, and the Sponsor.  
After completion of each dose escalation cohort, the Safety Review Committee (SRC) will 
review clinical safety and tolerability data as well as PK data available at the time for all 
patients in order to confirm dose escalation to the next dose level. Based on the SRC’s 
review, a cohort may be expanded, or an intermediate or a lower-than-planned dose level 
or an alternative dosing schedule may be evaluated.  
Determination of DLT for dosing Cohort B1 
Each dose cohort will include a minimum of 3 evaluable patients for assessment of DLT. In 
order to be considered evaluable for DLT, patients must meet at least one of the following 
criteria:  
1. Complete one 28-day cycle and receive at least 75% of MS-553 intended doses in 
Cycle 1;  
2. Discontinue due to DLT  
During the DLT evaluation period for each cohort, non-evaluable patients who discontinue 
treatment before the DLT evaluation period is complete for reasons other than study drug-
related toxicities (e.g., progressive disease, non-compliance), or do not receive at least 75% 
of MS-553 doses, will be replaced with additional patients enrolled at the same dose level. 
After a DLT is resolved or improved to  Grade 1, the study drug may continue at a lower 
dose level at the discretion of the investigator. 
A decision on dose escalation to the next dose level will be based on evaluation of the 
clinical safety profile and may also include evaluation of MS-553 exposure in treated 
patients. After the completion of each cohort, safety and PK data (if available) will be 
reviewed and a decision on dose escalation will be determined by the Investigators, the 
Safety Review Committee, and the Sponsor.  
After completion of each dose escalation cohort, the SRC will review clinical safety and 
tolerability data as well as PK data available at the time for all patients in order to confirm 
dose escalation to the next dose level. Based on the SRC’s review, a cohort may be 
expanded, or an intermediate (i.e., at 25 mg increment) or a lower-than-planned dose level 
or an alternative dosing schedule may be evaluated. 
Determination of DLT for dosing Cohort C1  
Each dose cohort will include a minimum of 3 evaluable patients for assessment of DLT. In 
order to be considered evaluable for DLT, patients must meet at least one of the following 
criteria:  
1. Complete the first four consecutive 28-day cycles and receive at least 75% of MS-553 
intended doses in these cycles  
2. Discontinue due to DLT  

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During the DLT evaluation periods for Cohort C1, non-evaluable patients who discontinue 
treatment before the DLT evaluation periods are complete for reasons other than study 
drug-related toxicities (e.g., progressive disease, non-compliance), or do not receive at least 
75% of MS-553 doses, will be replaced with additional patients enrolled at the same dose 
level. After a DLT is resolved or reversed to  Grade 1, the study drug may continue at a 
lower dose level at the discretion of the investigator. 
A decision on dose escalation to the next dose level will be based on evaluation of the 
clinical safety profile and may also include evaluation of MS-553 exposure in treated 
patients. After the completion of each cohort, safety and PK data (if available) will be 
reviewed and a decision on dose escalation will be determined by the Investigators, the 
SRC, and the Sponsor.  
After completion of each dose escalation cohort, the SRC will review clinical safety and 
tolerability data as well as PK data available at that time for all patients to confirm dose 
escalation to the next dose level. Based on the SRC’s review, a cohort may be expanded, or 
an intermediate (i.e., at 25 mg increment) or a lower-than-planned dose level or an 
alternative dosing schedule may be evaluated.  
Safety 
For Cohort A1, assessments for DLT and TEAE will occur during Cycle 1. For Cohort B1, 
assessments for DLT and TEAE will occur during Cycle 1 for dose levels up to and 
potentially including 225 mg BID. For Cohort C1, assessments for DLT and TEAE will occur 
from Cycle 1 through Cycle 4 for dose levels up to and potentially including 225 mg BID. 
TEAE will be assessed during the course of the trial. The primary safety endpoint will be 
the rate of DLT and TEAE requiring study drug discontinuation in the DLT evaluable period. 
Toxicities will be defined using NCI CTCAE v5.0 criteria. 
Efficacy 
Response assessments will be made by the individual investigators. Response for CLL will 
be evaluated according to 2008 criteria published by IWCLL. Because of the well-
documented lymphocytosis not associated with disease progression that occurs with BCR 
signaling inhibitors such as BTK inhibitors, progressive lymphocytosis in the absence of other 
signs of disease progression (e.g., splenomegaly, enlarging lymph nodes, disease-related 
constitutional symptoms) will not be considered disease progression. Isolated 
lymphocytosis in the context of what would otherwise be a PR is defined as a PR with 
lymphocytosis (noted as PR-L, PR+L, or PRwL in literature). Response criteria in the SLL 
patients and aggressive lymphoma patients will be based on the International Working 
Group consensus response evaluation criteria in lymphoma (RECIL 2017).  
Initial evaluation of the efficacy endpoints related to response will incorporate the data 
from the first 9 cycles of treatment. Any patient who receives at least one cycle of study 
therapy is evaluable for response. Patients who have stable disease or a response by 9 cycles 
and continue treatment after 9 cycles will have response assessments including CT scans of 
the chest, abdomen, and pelvis (and neck if involved), preferably every 12 weeks, or as 

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clinically indicated until disease progression. Patients who do not have a response by 9 
cycles but who have stable disease are eligible to stay on study treatment for up to a total 
of 24 cycles, with continual re-evaluation for response at subsequent visits during this time 
period. For patients who continue to have stable disease after 24 cycles or have a 
response after 9 cycles therapy, drug therapy can continue until disease progression.     
Exploratory 
 
 
 
 
Correlative Science 
Pharmacokinetic, pharmacodynamic, and exploratory analyses will be coordinated by 
MingSight. 
Specimens to be collected for correlative science studies include: 
Cohort A:  
Pre-treatment 
 
 
  
 
 
 
 
 
 
 
 
During Treatment 
 
 
 
 
 
9. Plasma for pharmacokinetic analysis. 
10. Blood for pharmacodynamic PKC-β biomarker analysis. 

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At disease progression in patients with response 
 
 
 
 
Cohort B: 
Pre-treatment 
 
 
  
    
During Treatment 
 
5. Plasma for pharmacokinetic analysis. 
 
7. Blood for pharmacodynamic PKC-β biomarker analysis. 
 
 
 
At disease progression in patients with response 
 
Cohort C: 
Pre-treatment 
 
 
During Treatment 
 
4. Plasma for pharmacokinetic analysis. 
 
6. Blood for pharmacodynamic PKC-β biomarker analysis. 

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At disease progression in patients with response 
 
Please refer to Section 6.3.2 for more detailed description and schedule of correlative 
science sample collections. 
Study Schema 
 
Diagnosis and main criteria for inclusion: 
 
 
 
 
Inclusion Criteria: To be eligible for inclusion in the dose escalation cohorts or expansion 
cohorts in this study, patients must meet all of the following criteria: 
1. Age 18 years or older. 
2. Diagnosis of CLL or SLL: 
a. History of histologically documented CLL or SLL that meets IWCLL diagnostic 
criteria according to the 2008 guidelines, and  
b. Indication for treatment as defined by the 2008 IWCLL guidelines, or the need for 
disease reduction prior to allogeneic transplantation. 
3. Cohort specific inclusion requirements: 
Cohort A1 (MS-553 monotherapy) 
Cohort A1
Monotherapy MS-553
Initial RP2D identified
Expansion Cohort A2 at A1 
initial RP2D
R/R CLL who
•
Have failed at least two 
prior lines of therapies, or
•
Have acquired resistance 
to a BTK inhibitor after 
achieving initial response
Expansion Cohort A3 at A1 
initial RP2D
Aggressive lymphoma 
including Richter’s, DLBCL, MCL
Cohort B1
MS-553 + acalabrutinib dose escalation
Starting at least one dose lower of monotherapy initial RP2D
Treatment experienced CLL patients:
•
BTKi naive
•
Eligible for BTKi as SOC
•
No more than 2 prior lines of therapies
Expansion Cohort B2 at B1 RP2D
Treatment experienced CLL patients:
•
BTKi naive
•
Eligible for BTKi as SOC
•
No more than 2 prior lines of 
therapies
Expansion Cohort B3 at B1 RP2D
Treatment experienced CLL patients:
•
BTKi naive
•
Eligible for BTKi as SOC
•
No more than 2 prior lines of 
therapies
•
TP53 abnormalities
Cohort C1
MS-553 + venetoclax + rituximab dose 
escalation starting at least one dose 
lower of monotherapy initial RP2D
•
Treatment experienced with no 
more than 2 lines of prior therapies
•
Eligible for SOC of venetoclax and 
rituximab
•
Naïve to venetoclax
Expansion Cohort C2 at C1 RP2D
•
Treatment experienced with no 
more than 2 lines of prior 
therapies
•
Eligible for SOC of venetoclax and 
rituximab
•
Naïve to venetoclax

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Relapsed after, or refractory to, progressing after at least two prior lines of therapies 
with the following exceptions: 
a. 
Patients with a 17p deletion who have already received venetoclax; or 
b. 
Patients who have TP53 mutation (prior therapy is not required) which shall 
be confirmed 
 at 
screening for enrollment. 
 
 
or 
c. 
Patients with clinical and/ or molecular (e.g., BTK and/or PLCG2 mutation) 
resistance after achieving initial response to a BTK inhibitor. 
• For patients without clinical resistance, molecular resistance (i.e., BTK 
and/or PLCG2 mutation) shall be confirmed 
 at 
screening for enrollment. 
Cohort A2 (MS-553 monotherapy) 
Relapsed/refractory CLL/SLL patients who have:   
a. Failed at least two prior lines of therapies, or  
b. Developed clinical and/or molecular (i.e., BTK and/or PLCG2 mutation) 
resistance after achieving an initial response to a BTK inhibitor. 
• For patients without clinical resistance, molecular resistance (i.e., BTK 
and/or PLCG2 mutation) shall be confirmed 
 assay at 
screening for enrollment. 
Cohort A3 (MS-553 monotherapy)  
See Inclusion Criterion #4 below. 
Cohort B1, B2 (MS-553 in combination with acalabrutinib)  
Treatment experienced patients with no more than two prior lines of therapies, who 
a. Are naïve to prior BTK inhibitor treatments, and  
b. Are eligible for single agent BTK inhibitor. 
Cohort B3 (MS-553 in combination with acalabrutinib) 
Treatment experienced patients receiving no more than two prior lines of therapies, 
who: 
a. 
Are naïve to prior BTK Inhibitor treatments, 
b. 
Are eligible for single agent BTK inhibitor, and 
c. 
Have 17p deletion or TP53 mutation which shall be confirmed 
 
 at screening for enrollment. (

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Cohort C1, C2 (triple regimen of MS-553 + rituximab + venetoclax) 
Treatment experienced patients receiving no more than 2 prior lines of treatment 
regimens, who: 
a. 
Are naïve to prior Bcl-2 inhibitor treatments, and  
b. 
Are eligible for venetoclax in combination with rituximab.  
4. For inclusion in the Cohort A3, patients must meet both of the following diagnostic 
criteria instead of inclusion criterion #2 or #3: 
a. Diagnosis of aggressive lymphoma, explicitly comprising Richter’s Transformation 
of CLL/SLL to Diffuse Large B-Cell Lymphoma (DLBCL); Activated B-cell (ABC) or 
non-Germinal Center B-cell (GCB) DLBCL not transformed from CLL/SLL, or MCL. 
Other histologies may be included after discussion with and approval of the study 
principal investigator and Sponsor medical monitor. 
b. Additionally, patients with MCL, or de novo ABC or non-GCB DLBCL patients must 
have received at least two lines of prior therapy and MCL patients must have failed 
at least a BTK inhibitor. Patients with Richter’s transformation have no such 
restriction on the use of prior therapy. 
5. WHO/ECOG performance status ≤ 2. 
6. Meet laboratory parameters (see Exclusion Criteria). 
7. In the opinion of the investigator, all of the following criteria are met:  
a. Have life expectancy ≥ 2 months  
b. Are willing to undergo all study-related evaluations and procedures, and 
c. Have ability to understand and willingness to execute a written informed consent 
document. 
Exclusion Criteria: Patients who meet any of the following criteria are not eligible for this 
study. 
1. Current or past transformation of CLL/SLL to prolymphocytic leukemia (PLL), non-
Hodgkin lymphoma, or Hodgkin lymphoma. 
a. Patients being considered for inclusion in the optional expansion cohort A3 
(aggressive lymphoma) are not excluded on the basis of transformed lymphoma, 
but must meet the definition of aggressive lymphoma outlined in the inclusion 
criteria for Cohort A3. 
2. Active and uncontrolled autoimmune cytopenia(s). 
3. Any of the following prior therapies within 14 days prior to Cycle 1 Day 1: 

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a. Major surgery 
b. Corticosteroids greater than 20 mg / day prednisone (or equivalent), unless used 
by inhalation, topical, or intra-articular routes, or unless necessary for 
premedication before and/or after iodinated contrast dye. Higher doses, and 
more prolonged courses of steroids are permitted under certain circumstances 
after discussion with the medical monitor and/or Sponsor including, but not 
limited to, the following: 
i. 
Treatment of disease-related autoimmune hemolysis or autoimmune 
thrombocytopenia; 
ii. 
Symptomatic relief of lymphadenopathy – preferably after completion of 
Cycle 1; 
iii. 
Symptomatic relief of B symptoms – after completion of Cycle 1; 
iv. 
Treatment of GVHD – please discuss with medical monitor; 
v. 
Short courses (up to 14 days) if needed for any intercurrent condition such 
as acute exacerbation of any non-disease-related condition (e.g., arthritis, 
asthma, COPD) including steroid dose modification required for adrenal 
insufficiency. 
c. Cytotoxic chemotherapy or biologic therapy, with the exception of BCR pathway 
kinase inhibitors for which only a 24-hour wash out prior to Cycle 1 Day 1 is 
required. 
4. Failure to recover: 
a. From clinically relevant toxicity to Grade 1 or less from prior chemo or 
radiotherapy or biologic therapy with the exception of alopecia, atrial fibrillation 
or hypertension that developed during treatment with a BTK inhibitor. 
b. to Grade 2 or less for atrial fibrillation or hypertension that occurred as a 
consequence of treatment with a BTK inhibitor. 
5. CNS leukemia/lymphoma – including history of treated CNS disease even if the patient 
is asymptomatic. 
6. Inadequate bone marrow function / hematopoietic reserve, except in the case of 
documented bone marrow involvement, evidenced as: 
a. Absolute Neutrophil Count (ANC) < 1 x 109/L 
b. Platelet count < 30 x 109/L 
7. Inadequate liver or renal function as specified below: 
a. Serum total bilirubin > 2 x ULN (upper limit of normal), or > 3 x ULN in case of liver 
involvement with CLL/SLL, or serum total bilirubin > 3 x ULN in patients with 
documented history of Gilbert’s disease and normal direct bilirubin. 

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b. AST or ALT > 2.5 x ULN, or > 5 x ULN if CLL/lymphoma is present in the liver  
c. Estimated GFR < 30 mL/min. 
8. For Cohort B patients, active treatment with strong CYP3A inhibitors, CYP3A inducers 
or proton pump inhibitors, unless medication can be stopped prior to enrollment in 
the study.  
9. For Cohort C patients, active treatment with strong and moderate CYP3A inhibitors, 
strong CYP3A inducers, or strong P-gp inhibitors, unless medication can be stopped 
prior to enrollment in the study.  
 
 
 
   
 
 
 
 
12. Receiving treatment with medications that are known to be strong inducers, inhibitors 
or sensitive substrates of CYP3A4/5 or medications with risk for QT prolongation that 
cannot be discontinued prior to study entry (at least 48 hours prior to the start of 
Cycle 1 Day 1 dosing). For any exceptions, discussion with medical monitor and/or 
Sponsor is needed. Weak or moderate inhibitors may be used with caution, except as 
otherwise specified in Exclusion Criteria. Examples of these medications are listed in 
Appendix 3. 
13. Clinically significant cardiac diseases, including any of the following: 
a. History or presence of ventricular tachyarrhythmia 
b. Presence of unstable/uncontrolled atrial fibrillation is exclusionary. Patients with 
stable atrial fibrillation (rate controlled pharmacologically) are eligible provided 
that they do not meet any other exclusion criteria. Patients whose atrial 
fibrillation occurred as a consequence of treatment with a BTK inhibitor may be 
eligible for the study provided that the patient is asymptomatic and on a stable 
dose of medication for rate control, and only after discussion with the medical 
monitor. 
c. Unstable angina pectoris or acute myocardial infarction within 3 months prior to 
start of study drug. 
d. NYHA functional class III or IV heart failure 
e. Baseline QTcF interval of > 480 msec. Prior QTc prolongation due to prior 
medication no longer on board is not exclusionary. A family history of QTc interval 
prolongation by itself is not exclusionary provided that the patient’s QTc is not 
prolonged. For those with prolonged QTc interval due to bundle branch block, 
patient may be eligible after documentation of a “corrected value” ≤480 msec and 
confirmation by cardiologist that patient is asymptomatic and that no other 

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cardiac conduction or cardiac abnormality is present that would pose any safety 
issue for the patient.  
Note: As the eligibility criteria specify the QT interval corrected by the method of 
Fridericia (QTcF), any correction for widened QRS should be sure to include a 
cube-root corrected R-R interval. Please discuss/consult with a cardiologist if 
there are any questions. 
14. History of another malignancy that in the opinion of the investigator limits survival to 
less than 2 years. 
 
 
 
 
 
17. Gastrointestinal dysfunction, including motility or malabsorption syndromes or 
inflammatory bowel disease which could limit absorption of MS-553. 
18. Known HIV positivity, or active hepatitis B or C infection with detectible viral nucleic 
acid in the blood. 
19. Severe systemic infections requiring intravenous antibiotics within the two weeks 
prior to initiation of MS-553. 
20. Any other life-threatening illness, medical condition, laboratory abnormalities, or 
therapies that in the opinion of the investigator could compromise the safety of the 
patient or interfere with analysis of study endpoints. 
21. Women who are lactating or pregnant. 
22. Women of child-bearing potential or male partners of women of child-bearing 
potential who will not agree to use highly effective method of contraception 
throughout the entire study period and for a minimum of 3 months after the last 
dose of study drug. Highly effective contraception methods include: 
a. Total abstinence or 
b. Male or female sterilization or 
c. Combination of any two of the following: 
i. 
Use of oral, injected or implanted hormonal methods of contraception 
ii. 
Placement of an intrauterine device (IUD) or intrauterine system (IUS) 
iii. 
Barrier methods of contraception: condom or occlusive cap (diaphragm or 
cervical/vault caps) with spermicidal foam/gel/film/cream/vaginal 
suppository. 
Women are considered post-menopausal and not of child-bearing potential if 
they have had 12 months of natural (spontaneous) amenorrhea with an 
appropriate clinical profile (age appropriate, history of vasomotor symptoms) 
or have had surgical bilateral oophorectomy (with or without hysterectomy) or 

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tubal ligation at least six weeks ago. In the case of oophorectomy alone, only 
when the reproductive status of the woman has been confirmed by follow up 
hormone level assessment is she considered not of childbearing potential. 
Women of child-bearing potential who will not agree to the following are 
ineligible for the study: 
• 
Refrain from breastfeeding and donating oocytes or blood during the 
course of the study and for 3 months after the last dose of study drug, 
and 
• 
Ongoing pregnancy testing during the course of the study and at the end 
of treatment visit.  
23. Men who will not agree to the following are ineligible for the study: 
• 
Refrain from donation or blood during the course of the study and for 3 
months after the last dose of study drug, and 
• 
Completely abstain from sexual contact, or must always use a latex or 
synthetic condom during any sexual contact until 3 months after the last 
dose of study drug, or have prior documentation of surgical sterilization. 
Investigational product, dosage and mode of administration in all Cohorts in A, B, C: 
MS-553 is administered orally, twice daily, in 28-day cycles. Dose levels include 100, 150, 200, 
225, 250 and 300 mg.   
Cohort B: Standard of care acalabrutinib will be administered according to the Full 
Prescribing Information in combination with MS-553. 
Cohort C: Standard of care rituximab will be administered beginning in Cycle 1, according to 
the Full Prescribing Information, in combination with MS-553 for two cycles. Beginning in 
Cycle 3, venetoclax will be started and the dose escalated from 20 mg to 400 mg over five (5) 
weeks in combination with rituximab and MS-553.  
Duration of treatment: Patients may continue receiving study drug provided they continue 
to tolerate the drug, have no evidence of progression, do not meet any of the criteria for 
discontinuation, and the patients’ participation in the study has not ended. The anticipated 
median treatment duration is unknown. 
Reference therapy, dosage and mode of administration: 
Not applicable. 

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Statistical methods: 
Summary statistics will be provided for all endpoints. Statistics will be reported according 
to intention-to-treat (ITT), per-protocol, and per-dose-level. The safety summary statistics 
will include any patient who received at least one dose of study drug. 
Patient characteristics will be described and summarized for each dose level and in total. 
Because disease-relevant pharmacodynamics biomarkers are not yet established, and 
some limited toxicity and pharmacokinetic data in healthy subjects exists, dose escalation 
will be guided by toxicity. 
There is no formal hypothesis to be tested in this study; therefore, no predefined sample size 
calculation has been made for the efficacy or exploratory endpoints. 
The protocol details dose modifications (i.e., reductions, interruptions) that may be 
warranted as a consequence of drug-related toxicities. At a starting dose level of 100 mg BID 
(Dose Level 0), no dose reduction is anticipated as monotherapy. If unacceptable toxicity 
presents during Cycle 1 at Dose Level 0 then the patient will be instructed to discontinue 
treatment with MS-553. If a patient experiences potential drug-related toxicity, the 
appropriate dose modification may be implemented until the toxicity has adequately 
resolved or improved to Grade 1. If unacceptable toxicity presents, then the patient will be 
instructed to discontinue treatment with MS-553. 
Formal stopping criteria are specified in Section 7.13. Since the study is unblinded, the 
Precision Oncology medical monitor, the Safety Review Committee, the relevant MS-553-103 
Investigator(s), and the Sponsor will closely review available safety and PK data on an 
ongoing basis throughout the dose escalation and dose expansion segments of the open-
label study.  

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TABLE OF CONTENTS, LIST OF TABLES, AND LIST OF FIGURES  
TABLE OF CONTENTS 
SYNOPSIS  ......................................................................................................................................4 
TABLE OF CONTENTS, LIST OF TABLES, AND LIST OF FIGURES ..................................................... 28 
LIST OF ABBREVIATIONS AND DEFINITIONS OF TERMS ................................................................ 34 
1. 
INTRODUCTION .......................................................................................................... 37 
1.1. 
Chronic Lymphocytic Leukemia ................................................................................. 37 
1.1.1. 
B Cell Receptor (BCR) Signaling Inhibitors ................................................................. 37 
1.1.2. 
Protein Kinase C and PKC-β ....................................................................................... 38 
1.1.3. 
PKC in the Microenvironment ................................................................................... 38 
1.1.4. 
Autoimmunity ............................................................................................................ 39 
1.2. 
MS-553 ....................................................................................................................... 39 
1.2.1. 
Mechanism of Action ................................................................................................. 39 
1.2.2. 
Safety Pharmacology ................................................................................................. 40 
1.2.3. 
Metabolism and Drug-Drug Interaction Potential ..................................................... 40 
1.2.4. 
Summary of Clinical Experience with MS-553 ........................................................... 40 
2. 
OBJECTIVES AND ENDPOINTS .................................................................................... 42 
2.1. 
Cohort A Objectives ................................................................................................... 42 
2.1.1. 
Primary Objective ...................................................................................................... 42 
2.1.2. 
Secondary Objective .................................................................................................. 42 
2.1.3. 
Exploratory Objectives ............................................................................................... 42 
2.2. 
Cohort B Objectives ................................................................................................... 43 
2.2.1. 
Primary Objective ...................................................................................................... 43 
2.2.2. 
Secondary Objectives................................................................................................. 43 
2.2.3. 
Exploratory Objectives ............................................................................................... 43 
2.3. 
Cohort C Objectives ................................................................................................... 43 
2.3.1. 
Primary Objective ...................................................................................................... 43 
2.3.2. 
Secondary Objectives................................................................................................. 43 
2.3.3. 
Exploratory Objectives ............................................................................................... 44 
2.4. 
Primary Endpoints ..................................................................................................... 44 
2.5. 
Secondary Endpoints ................................................................................................. 44 

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2.6. 
Exploratory Endpoints ............................................................................................... 45 
3. 
INVESTIGATIONAL PLAN ............................................................................................ 47 
3.1. 
Overview of Study Design .......................................................................................... 47 
3.1.1. 
Cohort A: MS-553 Monotherapy ............................................................................... 47 
3.1.2. 
Cohort B: MS-553 in combination with acalabrutinib ............................................... 48 
3.1.3. 
Cohort C: MS-553 in combination with venetoclax and rituximab ........................... 49 
3.1.4. 
Study Design Schematic ............................................................................................. 49 
3.2. 
Treatment Duration ................................................................................................... 49 
3.3. 
Dose Escalation .......................................................................................................... 50 
3.3.1. 
Dose Escalation: Monotherapy Cohort A1 ................................................................ 50 
3.3.2. 
Dose Escalation: General Principles........................................................................... 51 
3.3.3. 
Dose Escalation: Combination Cohort B1 .................................................................. 53 
3.3.4. 
Dose Escalation: Combination Cohort C1 .................................................................. 53 
3.3.5. 
Dose Limiting Toxicity Definition ............................................................................... 54 
3.4. 
Dose Expansion Cohorts ............................................................................................ 55 
3.4.1. 
Expansion Cohort A2: Monotherapy ......................................................................... 55 
3.4.1.1. 
Optional Expansion Cohort A3: Monotherapy .......................................................... 55 
3.4.2. 
Expansion Cohorts B: Combination with Acalabrutinib ............................................ 55 
3.4.2.1. 
Expansion Cohort B2 .................................................................................................. 55 
3.4.2.2. 
Expansion Cohort B3 .................................................................................................. 56 
3.4.3. 
Expansion Cohorts C: Combination of Venetoclax and Rituximab ............................ 56 
3.4.3.1. 
Expansion Cohort C2 .................................................................................................. 56 
3.5. 
Safety Review Committee.......................................................................................... 56 
3.6. 
Remaining on Treatment ........................................................................................... 56 
3.7. 
Response Assessment ................................................................................................ 57 
4. 
PATIENT POPULATION ............................................................................................... 58 
4.1. 
Inclusion Criteria ........................................................................................................ 58 
4.2. 
Exclusion Criteria ....................................................................................................... 60 
5. 
STUDY TREATMENT ................................................................................................... 64 
5.1. 
Overall Study Design Investigational Study Drug ...................................................... 64 
5.1.1. 
MS-553 Treatment Description ................................................................................. 64 

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5.1.1.1. 
Storage and Handling................................................................................................. 64 
5.1.1.2. 
Study Drug Disposal ................................................................................................... 64 
5.1.2. 
Combination Treatment Description ......................................................................... 64 
5.2. 
Treatment Plan .......................................................................................................... 64 
5.2.1. 
Premedication ............................................................................................................ 64 
5.2.2. 
Administration Instructions ....................................................................................... 65 
5.2.3. 
Dosing Instructions and Schedule.............................................................................. 65 
5.2.3.1. 
MS-553 Dosing – All Cohorts ..................................................................................... 65 
5.2.3.2. 
Acalabrutinib Dosing – B Cohorts .............................................................................. 65 
5.2.3.3. 
Venetoclax and Rituximab Dosing – C Cohorts ......................................................... 65 
5.2.4. 
Concomitant and Supportive Therapy ....................................................................... 66 
5.2.4.1. 
MS-553 ....................................................................................................................... 66 
5.2.4.2. 
Acalabrutinib .............................................................................................................. 66 
5.2.4.3. 
Venetoclax ................................................................................................................. 66 
5.2.4.4. 
Anti-CD20 mAb .......................................................................................................... 67 
5.2.5. 
Excluded Medications ................................................................................................ 68 
5.3. 
Withdrawal Criteria ................................................................................................... 69 
5.4. 
Discontinued Patients ................................................................................................ 69 
6. 
STUDY PROCEDURES .................................................................................................. 70 
6.1. 
Description of Study Procedures ............................................................................... 70 
6.1.1. 
Patient Enrollment and Treatment Assignment ........................................................ 70 
6.1.2. 
Screening Period ........................................................................................................ 70 
6.1.3. 
Informed Consent ...................................................................................................... 70 
6.1.4. 
Disbursement of Study Medication ........................................................................... 70 
6.1.4.1. 
Disbursement of MS-553 Study Medication ............................................................. 70 
6.1.4.2. 
Disbursement of Acalabrutinib, Venetoclax, and Rituximab..................................... 71 
6.1.5. 
Instruction to the Patient for Administration ............................................................ 71 
6.1.6. 
Study Drug Accountability and Treatment Compliance ............................................ 71 
6.1.7. 
Dose Modifications and Dose Delay .......................................................................... 71 
6.1.8. 
Permitted Concomitant Therapy ............................................................................... 72 
6.1.9. 
Permitted Concomitant Therapy to be Used with Caution ....................................... 73 

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6.1.10. 
Prohibited Concomitant Medications ....................................................................... 73 
6.1.11. 
Visit Schedule ............................................................................................................. 74 
6.1.12. 
Visit Days and Numbering.......................................................................................... 74 
6.2. 
Assessments of Safety, Efficacy, and Exploratory Endpoints .................................... 74 
6.2.1. 
Safety ......................................................................................................................... 74 
6.2.2. 
Efficacy ....................................................................................................................... 75 
6.2.3. 
Pharmacokinetics and Exploratory ............................................................................ 76 
6.3. 
Correlative Science .................................................................................................... 77 
6.3.1. 
Responsibilities .......................................................................................................... 77 
6.3.2. 
Correlative Sciences Sample Collection Overview .................................................... 77 
6.3.3. 
Correlative Science Studies Description .................................................................... 79 
7. 
ADVERSE EVENTS AND TOXICITY MANAGEMENT ..................................................... 81 
7.1. 
Dose Limiting Toxicities ............................................................................................. 81 
7.2. 
Modification or Holding of Dose for Toxicities .......................................................... 81 
7.3. 
Adverse Events........................................................................................................... 81 
7.4. 
Serious Adverse Event ............................................................................................... 82 
7.5. 
Definition and Grading of Toxicities .......................................................................... 83 
7.6. 
Causality of Adverse Events ....................................................................................... 83 
7.7. 
Documentation of Adverse Events and Serious Adverse Events .............................. 83 
7.7.1. 
Reporting Period ........................................................................................................ 83 
7.7.2. 
Assessment of Adverse Events .................................................................................. 83 
7.8. 
Procedures for Reporting Serious Adverse Events .................................................... 84 
7.8.1. 
Completion and Transmission of SAE Report ............................................................ 84 
7.8.2. 
Regulatory Reporting Requirements for Serious Adverse Events ............................. 84 
7.9. 
Pregnancy .................................................................................................................. 85 
7.10. 
Follow Up of Adverse Events and Serious Adverse Events........................................ 85 
 
 
7.12. 
Management of Overdose and Toxicity .................................................................... 86 
7.13. 
Study-wide Stopping Rule for Excessive Toxicity ....................................................... 86 
8. 
STATISTICAL CONSIDERATIONS AND DATA ANALYSIS ............................................... 88 
8.1. 
General Considerations ............................................................................................. 88 

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8.2. 
Sample Size Calculations ............................................................................................ 88 
8.3. 
Dose Escalation Criteria ............................................................................................. 88 
8.4. 
Determination of Sample Size ................................................................................... 88 
8.5. 
Analysis Populations .................................................................................................. 88 
8.5.1. 
Intention to Treat....................................................................................................... 88 
8.5.2. 
Per Protocol Population ............................................................................................. 88 
8.5.3. 
Pharmacokinetic Population ...................................................................................... 88 
8.5.4. 
Pharmacodynamic Population ................................................................................... 89 
8.6. 
Demographic Data ..................................................................................................... 89 
8.7. 
Summary Statistics ..................................................................................................... 89 
8.8. 
Secondary Analyses: Efficacy ..................................................................................... 89 
8.9. 
Exploratory Analyses.................................................................................................. 89 
8.10. 
Protocol Deviations and Violations............................................................................ 90 
8.11. 
Data Recording .......................................................................................................... 90 
8.12. 
Data Quality Control .................................................................................................. 90 
9. 
REGULATORY AND ETHICAL CONSIDERATIONS ......................................................... 91 
9.1. 
Summary Statistics Regulatory and Ethical Compliance: Good Clinical 
Practice ...................................................................................................................... 91 
9.2. 
Institutional Review Board/Independent Ethics Committee .................................... 91 
9.3. 
Informed Consent ...................................................................................................... 92 
9.4. 
Amendments to the Protocol .................................................................................... 92 
9.5. 
Files and Records Retention ...................................................................................... 92 
9.6. 
Publication of Results ................................................................................................ 93 
9.7. 
Disclosure and Confidentiality ................................................................................... 93 
9.8. 
DEFINITION OF END OF TRIAL .................................................................................... 94 
9.8.1. 
End of Trial Participation in a Member State of the European Union ...................... 94 
9.8.2. 
End of Trial in All Other Participating Countries ........................................................ 94 
10. 
REFERENCES ............................................................................................................... 95 
11. 
APPENDICES ............................................................................................................... 97 
APPENDIX 1A: STUDY CALENDAR A COHORTS (MS-553 MONOTHERAPY)................................... 98 
APPENDIX 1B: STUDY CALENDAR B COHORTS (ACALBRUTINIB + MS-553) ................................ 101 

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APPENDIX 1C: STUDY CALENDAR C COHORTS (TRIPLET REGIMEN MS-553 + RITUXIMAB 
+ VENETOCLAX) ........................................................................................................ 104 
APPENDIX 2A: MS-553 RECOMMENDED DOSE MODIFICATIONS AND DOSE DELAYS ............... 108 
APPENDIX 2B: DOSE MODIFICATIONS OF ACALABRUTINIB........................................................ 112 
APPENDIX 2C: DOSE MODIFICATIONS OF VENETOCLAX AND RITUXIMAB ................................. 113 
 
APPENDIX 3: LIST OF CO-MEDICATIONS TO AVOID .................................................................... 117 
APPENDIX 4: NCI CTCAE V 5.0 AND IWCLL GUIDELINES (2008 & 2018) ..................................... 119 
APPENDIX 5: CLL/SLL RESPONSE ASSESSMENTS ......................................................................... 120 
 
LIST OF TABLES 
Table 1: 
Abbreviations ............................................................................................................. 34 
Table 2:  
Monotherapy Dose Levels ......................................................................................... 50 
Table 3:  
Recommended Phase 2 Dose .................................................................................... 52 
Table 4:  
Dosing Schedule and DLT Evaluation Periods for Cohort B1 ..................................... 53 
Table 5:  
Dosing Schedule and DLT Evaluation Periods for Cohort C1 ..................................... 53 
Table 6:  
Recommended TLS Prophylaxis Based on Tumor Burden in Patients with 
CLL/SLL ....................................................................................................................... 67 
Table 7:  
Schedule of PK Assessments ...................................................................................... 76 
Table 8:  
Schedule of Correlative Sciences Sample Collections ............................................... 78 
  
LIST OF FIGURES 
Figure 1: 
Study Schema............................................................................................................. 49 
 

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LIST OF ABBREVIATIONS AND DEFINITIONS OF TERMS 
The following abbreviations and specialist terms are used in this study protocol. 
Table 1: 
Abbreviations 
Abbreviation or Specialist Term 
Definition 
AE 
Adverse Event 
ALT 
Alanine aminotransferase/glutamic pyruvic 
transaminase/SGPT 
ANC 
Absolute Neutrophil Count 
AST 
Aspartate aminotransferase/glutamic oxaloacetic 
transaminase/SGOT 
AUC 
Area under the curve 
BCR 
B-cell receptor 
BID 
bis in die/twice a day 
BTK 
Bruton’s Tyrosine Kinase 
CBC 
Complete Blood Count 
CK 
Creatine Phosphokinase 
CI 
Confidence Interval 
CLL 
Chronic lymphocytic leukemia 
Cmax 
Maximum (peak) concentration of drug 
Cmin 
Minimum (peak) concentration of drug 
CR 
Complete Response 
CT 
Computed Tomography 
CTC 
Circulating Tumor Cells 
CTCAE 
Common Terminology Criteria Adverse Events 
DLT 
Dose Limiting Toxicity 
DoR 
Duration of Response 
DLBCL 
Diffuse Large B-cell Lymphoma 
ECG 
Electrocardiogram 
EOT 
End of Treatment 
FFPE 
Formalin-Fixed Paraffin-Embedded 
GCB 
Germinal Center B-Cell 

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Abbreviation or Specialist Term 
Definition 
GCP 
Good Clinical Practice 
IB 
Investigator’s Brochure 
ICH 
International Council for Harmonization 
IRB 
Institutional Review Board 
IWCLL 
International Workshop on Chronic Lymphocytic 
Leukemia 
LDH 
Lactate Dehydrogenase 
 
 
MAD 
Multiple Ascending Dose 
MCL 
Mantle Cell Lymphoma 
MRD 
Minimal Residual Disease 
MTD 
Maximum Tolerated Dose 
NGS 
Next-generation sequencing 
ORR 
Overall Response Rate 
OS 
Overall Survival 
PD 
Progressive Disease 
PFS 
Progression Free Survival 
PK 
Pharmacokinetics 
PKC 
Protein Kinase C 
PLCG2 
Phospholipase C gamma 2 
PR 
Partial Response 
PRwL 
Partial Response with lymphocytosis  
QD 
Quaque die/ once a day 
QTCF 
QT interval, corrected by Fridericia method 
RP2D 
Recommended Phase 2 Dose 
SAD 
Single Ascending Dose 
SAE 
Serious Adverse Event 
SD 
Stable Disease 
SLL 
Small lymphocytic lymphoma 

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Abbreviation or Specialist Term 
Definition 
SRC 
Safety Review Committee 
TBD 
To be determined 
TEAE 
Treatment Emergent Adverse Event 
TLS 
Tumor Lysis Syndrome 
TP53 
Tumor Protein P53 
Tmax 
Time to reach maximum (peak) concentration of drug 
t1/2 
Terminal half-life 
WBC 
White Blood cells 
WHO 
World Health Organization 

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1. 
INTRODUCTION 
1.1. 
Chronic Lymphocytic Leukemia 
Chronic lymphocytic leukemia (CLL) is the most common leukemia in United States adults, 
accounting for about one third of all new cases of leukemia as well as one third of all leukemia 
deaths.
1 CLL as a cancer is defined by an excess of clonal, abnormal B lymphocytes, 
accumulation of which was historically believed to be solely (at least in early stage disease) due 
to evasion of apoptosis, although we now know that that there is a significant proliferative 
component to CLL
2,3 frequently in the protective and nurturing microenvironment of 
hematopoietic tissues, such as the bone marrow and lymph node. 
Traditionally, therapy for CLL has consisted of a sequence or combination of cytotoxic 
chemotherapy and monoclonal antibody immunotherapy. Response rates for front line chemo-
immunotherapy therapy are good, and can exceed 90%, but relapse is inevitable and CLL is 
therefore currently considered an incurable disease. Patients who relapse after initial chemo-
immunotherapy at present have limited treatment options outside the context of a clinical trial, 
and responses to agents in second line and beyond are often fair at best. Furthermore, the 
molecular biology—and hence the clinical course— varies significantly among patients, and of 
patients with poor-risk genetic features no currently approved therapies seem to produce 
durable and robust responses. With a view to improve response rates and response durability 
among all patients, including high-risk patients, and with an increased understanding of the 
mechanisms of CLL, recent clinical work has shifted toward targeted therapy with small 
molecules and engineered antibodies: there have been a number of successes in targeting key 
points in B-cell receptor (BCR) signaling and the NF-κB cascade, as well as various cell-surface 
markers, especially CD20. 
1.1.1. 
B Cell Receptor (BCR) Signaling Inhibitors 
Because activated BCR signaling seems to be a sine qua non of CLL, much work has been 
devoted to targeting components of this pathway. The BCR complex itself is a transmembrane 
immunoglobulin molecule partnered with a CD79 heterodimer. Ligation of the BCR results in 
phosphorylation of an intracytoplasmic domain of CD79, which then signals through multiple 
pathways, notably through BTK (Bruton’s Tyrosine Kinase), PLCG2, and PI3K. Upregulation of 
genes involved in these pathways has been observed in CLL and represent targets.4 In fact, 
targeting BTK as a component of the BCR pathway has shown enormous promise in multiple 
clinical trials and has led to FDA approval of the first-in-class BTK in both the upfront and 
relapsed setting. 5,6 
However, because of tumor heterogeneity, genomic instability, and historic experience with 
other targeted agents, it is not surprising that CLL would develop resistance mutations in 
response to prolonged monotherapy with a BTK inhibitor. A recent report of 3 patients who 
had progressed on ibrutinib utilized next-generation sequencing techniques to look for 
mutations arising from exposure to the BTK inhibitor.
7 Investigators discovered two mutations 
in BTK and one constitutive gain-of-function mutation in PLCG2, a substrate of BTK. We have 
subsequently identified additional mutations in PLCG2 among ibrutinib-treated patients. 

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1.1.2. 
Protein Kinase C and PKC-β 
Immediately downstream of PLCG2 lies Protein Kinase C beta (PKC-β), at the confluence of the 
BCR pathway, the NF-κB cascade, and the RAS/RAF/MEK/ERK pathway; PKC-β is thus a critical 
downstream effector in BCR signaling. In healthy B lymphocytes, PKC-β is important in the 
transduction of normal BCR signals. However, it has been well-demonstrated that constitutive 
activation of BCR signaling plays a key role in the maintenance of the CLL cell,8 and as a 
consequence of this it stands to reason that PKC-β therefore occupies a position of both 
considerable import and opportunity. By acting immediately downstream of the BTK and PI3K 
components of the BCR signaling pathway, modulation of PKC-β has the potential to bypass 
mechanisms of resistance effected by mutations in these genes, and targeting PKC-β in this 
patient population is a rational and highly promising strategy. 
PKC-β, encoded by the gene PRKCB, is a member of a large family of protein kinases responsible 
for phosphorylation of serine and threonine residues of downstream effector proteins; PKC-β is, 
like the other two other members of the “conventional” protein kinase C subfamily PKC-α and 
PKC-γ, activated by the second messengers diacylglycerol and Ca2+, whereas members of the 
novel subfamily (PKC delta, eta, epsilon, and theta) require only diacylglycerol.9 While PKC 
family proteins are present across many cell types, normal and malignant B lymphocytes express 
only a few of these at a significant level, including PKC-β and PKC-δ. PKC-β is exceptional among 
the others, however, for being much more highly expressed in CLL cells compared to normal B 
cells: PRKC-β mRNA transcripts are up to 8 fold higher in some CLL cells than in normal B cells  in 
our unpublished observations, and others have shown that the protein product exists as a 
higher proportion of total cellular protein compared to normal B cells. 10 This is likely reflective 
of the combination of high levels of BCR signaling in CLL compared to normal B cells and the 
critically of PKC-β within this network. Not surprisingly, the previously mentioned study and 
others have shown that in vitro inhibition of PKC-β has a detrimental effect on CLL cell  
survival. 10-12 Indeed, TCL1tg, PKC-β null mice even failed to develop leukemia. 13 Other forms of 
PKC likely also play a part in the signal transduction that sustains CLL: the delta form of PKC 
(PKC-δ) has also been shown to interact with PI3K in CLL cells and inhibition of PKC-δ enhanced 
apoptosis of CLL cells, but not normal B cells. 14 
1.1.3. 
PKC in the Microenvironment 
PKC inhibition appears to have relevance beyond the tumor cell itself, extending also into the 
microenvironment. Experiments with Prkcb knockout mice demonstrated the importance of 
PKC-β and subsequent NF-κB signaling in the microenvironment: CLL-like leukemia cells from 
TCL1tg mice would not engraft in Prkcb-/- mice whereas wild-type mice succumbed to 
disease. 15 
The possibility that systemic inhibition of PKC even in non-tumor tissues could provide 
additional benefit thus provides further rationale for the use of PKC inhibitor in CLL. Separate 
from this, we have found that the PKC inhibitor sotrastaurin also activates GSK-β via 
dephosphorylation in the 2-5 µM range with subsequent decrease in B-catenin levels with 
concomitant decrease in Myc, Cyclin D1, and Mcl-1 expression. Given that previous studies have 
demonstrated that the WNT/β- catenin levels are active in CLL and also have frequent 

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mutations, pursuing PKC inhibitors as therapeutic agents targeting WNT is worthwhile and may 
provide a pharmacodynamic endpoint to study the effect of this therapy in vivo. 
1.1.4. 
Autoimmunity 
Besides the role of PKC-ß in transducing the overactive BCR signals in CLL cells, the PKC family of 
kinases has been shown to be crucial to the regulation and normal function of both normal T 
cell and B cell lymphocytes, and their contribution to autoimmune diseases. The three isoforms, 
PKC-θ, PKC-α and PKC-β, appear to be most important for lymphocyte function. PKC-θ is 
downstream of the T cell receptor complex and is required for effector T cell activation, 
function, survival, and autoimmune stimulation. Interestingly, PKC-θ negatively regulates the 
function of regulatory T cells and therefore inhibition of PKC-θ enhances the function of 
regulatory T cells. PKC-α plays a non- redundant role and collaborates with PKC θ in T effector 
cell activation and function. As in CLL, PKC-β plays a key role in B cell activation, function, 
survival, and the dysfunction seen in autoimmunity. 
Interestingly, inhibition of PKC-δ, in contrast to other isoforms, appears to have the potential to 
induce autoimmune disease in B cells. Previous PKC inhibiting compounds (e.g., enzastaurin, 
sotrastaurin) inhibit PKC-δ as well as PKC-ß. MS-553, a novel PKC-ß inhibitor lacks PKC-δ 
inhibition while selectively inhibiting PKC-ß  and to slightly lesser extent PKC-α and PKC-θ. These 
features of MS-553 effectively combine to downregulate autoimmune stimulation of B and T 
cells while enhancing the function of regulatory T cells. Avoidance of autoimmunity is key, 
because complications related to enhanced T cell function including fatal pneumonitis and 
colitis have been reported with other kinase inhibitors in CLL and lymphoma. 
1.2. 
MS-553 
MS-553 (chemical name: ((2S,5R)-2,5-dimethyl-4-((tetrahydro-2H-pyran-4- yl)methyl)piperazin-
1-yl)(3-(5-fluoro-2-methylpyrimidin-4-ylamino)-6,6-dimethylpyrrolo[3,4-c]pyrazol-5(1H,4H,6H)-
yl)methanone) is a selective inhibitor of PKC activity. It has a molecular weight of 500.6 g/mol 
and is orally bioavailable in humans. For clinical testing it can be formulated with high purity to 
GMP standards. MS-553 is an investigational product and has not been approved for marketing 
in any country. 
1.2.1. 
Mechanism of Action 
Nonclinical pharmacology studies have demonstrated that MS-553 is a potent, ATP- 
competitive and reversible inhibitor of several classical PKC enzyme isoforms with a Ki =5.3 nM 
for recombinant PKC-β and a Ki = 10.4 nM for recombinant PKC-α. It also is a potent inhibitor 
of the novel isoform PKC-θ with an IC50 = 25.6 nM. MS-553 does not inhibit PKC-δ (IC50 > 1000 
nM). Additionally, screening a broad panel of protein kinases reveals that MS-553 is very 
selective against kinases other than PKC.  
MS-553 has been shown to be efficacious in several cell lines and a xenograft model of diffuse 
large B cell lymphoma that are driven by chronic activation of BCR-NFκB signaling due to CD79 
mutation. In addition, several nonclinical studies also demonstrated that MS-553 is effective in 

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autoimmune disease models of lupus, rheumatoid arthritis, multiple sclerosis, and uveitis. For 
more information on the pharmacology see the MS-553 Investigator’s Brochure (IB). 
1.2.2. 
Safety Pharmacology 
A core battery of safety studies performed in nonhuman animals (rat; dog) indicated no 
undesirable pharmacodynamics effects of MS-553 on physiological functions in relation to 
exposure in the therapeutic range and above. No functional changes in vital organs or systems 
which are likely to be of importance in clinical testing of MS-553 were identified. 
  
 
 
 
 
1.2.3. 
Metabolism and Drug-Drug Interaction Potential 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
1.2.4. 
Summary of Clinical Experience with MS-553 
MS-553 has been explored in single ascending dose (SAD) (protocol 2014-04-001) and 
multiple ascending dose (MAD) (protocol MS-553-102) studies in healthy human volunteers. 
MS-553 has not been studied in patients with cancer or other immune system diseases. 
The Phase 1 SAD study evaluated doses ranging from 25 mg to 400 mg. Pharmacokinetic (PK) 
data from this study demonstrated that MS-553 was rapidly absorbed into systemic circulation 
with a Tmax of 2-4 hours and was rapidly eliminated with a mean apparent terminal half-life (t ½) 

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of 10-14 hours. The t ½ did not appear to be dose dependent. The inter-subject variability in 
exposure measures (Cmax and AUC) and the t ½ appears to be low. Increases in dosage led to 
mostly dose proportional increases in exposure. The PK behavior of MS-553 was similar when 
administered in either a fed or a fasted condition. While food did not change the PK profile of 
MS-553, gastrointestinal side effects decreased substantially when MS-553 is taken with food, 
as demonstrated in the MAD study. 
In the SAD study, MS-553 was well tolerated; AEs were either mild or moderate and were 
observed predominantly in the highest active dose group (400 mg). The most frequently 
observed adverse events were nausea (9 subjects, 19% of subjects who received active drug), 
vomiting (4 subjects, 8% of subjects who received active drug), visual disturbance (4 subjects, 
8% of subjects who received active drug), dizziness/vertigo (4 subjects, 8% of subjects who 
received active drug) and headache (3 subjects, 6% of subjects who received active drug). All 
AEs were transient and resolved. Refer to the IB for additional details. 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
In the MAD study, MS-553 was well tolerated and all AEs were either moderate or mild. The 
most frequent observed AEs were headache (9 subjects, 37.5% of subjects receiving active 
drug), nausea (3 subjects, 12.5% of subjects receiving active drug), visual disturbance (3 
subjects, 12.5% of subjects receiving active drug), neck pain (3 subjects, 12.5% of subjects 
receiving active drug), abdominal pain (3 subjects, 12.5% of subjects receiving active drug) and 
dizziness (2 subjects, 8.3% of subjects receiving active drug). All AEs were transient and 
resolved. Refer to the IB for additional details. 

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2. 
OBJECTIVES AND ENDPOINTS 
2.1. 
Cohort A Objectives 
2.1.1. 
Primary Objective 
The primary objective of Cohort A is to: 
• Evaluate the safety of MS-553 monotherapy in patients with CLL/SLL whose disease 
relapsed after or was refractory to at least two prior lines of therapies with the following 
exceptions: 
o Patients with a 17p deletion who have already received venetoclax, or 
o Patients who have TP53 mutation (prior therapy is not required for these 
patients), or  
o Patients who have either acquired clinical and/ or molecular (e.g., BTK or PLCG2 
mutation) resistance to a BTK inhibitor.  
• Determine the MTD or the initial RP2D of MS-553 monotherapy. 
2.1.2. 
Secondary Objective 
The secondary objective of Cohort A is to: 
• Evaluate the clinical activity of MS-553 in patients with relapsed or refractory 
CLL/SLL who 
o Have failed at least two prior lines of therapies, or 
o Have either acquired clinical and/ or molecular (e.g., BTK or PLCG2 
mutation) resistance to a BTK inhibitor. 
• Evaluate pharmacokinetics in patients receiving MS-553. 
Pursuit of the secondary objective should not jeopardize the primary objective, which is safety. 
2.1.3. 
Exploratory Objectives 
Exploratory objectives of Cohort A include the following: 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

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• 
 
 
2.2. 
Cohort B Objectives 
2.2.1. 
Primary Objective 
The primary objective of Cohort B is to: 
• Evaluate the safety of MS-553 in combination with acalabrutinib in patients with 
CLL/SLL. 
• Determine the MTD or the initial RP2D of MS-553 in combination with acalabrutinib.  
2.2.2. 
Secondary Objectives 
The secondary objectives of Cohort B are to: 
• Evaluate the clinical activity of MS-553 in combination with acalabrutinib in patients 
with CLL/SLL.  
• Evaluate pharmacokinetics in patients receiving MS-553 in combination with 
acalabrutinib. 
Pursuit of the secondary objectives should not jeopardize the primary objective, which is safety. 
2.2.3. 
Exploratory Objectives 
Exploratory objectives of Cohort B include the following: 
• 
 
 
 
 
2.3. 
Cohort C Objectives 
2.3.1. 
Primary Objective 
The primary objectives of Cohort C are to: 
• Evaluate the safety of MS-553 in combination with venetoclax and rituximab in patients 
with CLL/SLL. 
• Determine the MTD or the initial RP2D of MS-553 in combination with venetoclax and 
rituximab.  
2.3.2. 
Secondary Objectives 
The secondary objectives of Cohort C are to: 
• Evaluate the clinical activity of MS-553 in combination with venetoclax and rituximab in 
patients with CLL/SLL.  
• Evaluate pharmacokinetics in patients receiving MS-553 in combination with venetoclax 
and rituximab. 

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Pursuit of the secondary objectives should not jeopardize the primary objective, which is safety. 
2.3.3. 
Exploratory Objectives 
Exploratory objectives of Cohort C include the following: 
 
 
 
 
 
2.4. 
Primary Endpoints 
The primary endpoints of this study include: 
• Safety 
o The incidence rate of dose-limiting toxicities (DLT) and treatment-emergent adverse 
events (TEAE) requiring study drug discontinuation. Assessments for DLT and TEAE 
will occur during Cycle 1 for Cohort A1, and during the protocol defined DLT periods 
for Cohort B1 and Cohort C1 (see Sections 0 and 3.3.4). TEAE will be assessed 
throughout the course of the trial. The primary endpoint will be the rate of DLT and 
TEAE requiring study drug discontinuation in the respective DLT evaluation periods 
of Cohort A1, Cohort B1, and Cohort C1. 
• MTD or initial RP2D as monotherapy, as well as in combination with either acalabrutinib 
or venetoclax and rituximab. 
2.5. 
Secondary Endpoints 
Secondary endpoints in this study are related to clinical activity. These include the following: 
• Objective response rate (ORR), with overall response defined as best response of 
complete response (CR), CR with incomplete marrow recovery (CRi), partial response 
(PR), or PR with lymphocytosis (PRwL) according to the 2008 International Workshop on 
Chronic Lymphocytic Leukemia Response Criteria (IWCLL) with modifications for 
treatment-related lymphocytosis.  
• Duration of response (DoR), defined as time from first documentation of response to 
first documentation of progressive disease (PD) per 2008 IWCLL, first new therapy 
including allogeneic transplantation, or death due to any cause. 
• Progression free survival (PFS), defined as time from first dose of study treatment to the 
first documentation of PD, first new therapy excluding allogeneic transplantation, or 
death due to any cause. 
• Overall survival (OS), defined as time from first dose of study treatment to death due to 
any cause. 
• 
Disease control rate (DCR) defined as the percentage of patients who have achieved 
CR, CRi, PR, PRwL and stable disease (SD). Duration of stable disease with stable 
disease defined as at least 6 months from the start of MS-553 monotherapy to first 

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documentation of progressive disease (PD) in relapsed and refractory CLL/SLL 
patients. 
• Pharmacokinetics of MS-553 monotherapy, as well as the small molecule agents used in 
combination, acalabrutinib, or venetoclax. 
2.6. 
Exploratory Endpoints 
Cohort A:  
 
 
 
 
 
 
  
 
 
 
 
 
 
 
 
 
 
 
  
 
 
 
 
 
 
  
 
 
 
 
 
 
 
 
 
 
 
 
 
  
 
 
 
 

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Cohort B:  
Samples: For Cohort B, the samples being collected in this study for the measurement of 
exploratory endpoints are: 
 
  
 
 
 
 
 
 
 
 
Cohort C:  
Samples: For Cohort C, the samples being collected in this study for the measurement of 
exploratory endpoints are: 
 
  
  
 
 
 
 
 
 
 

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3. 
INVESTIGATIONAL PLAN 
3.1. 
Overview of Study Design 
This is a Phase I/II multi-center, open-label study of single agent MS-553, as well as MS-553 in 
combination with acalabrutinib or venetoclax and rituximab in treatment experienced CLL/SLL 
patients.  
The study will consist of 3 cohorts. Cohort A will evaluate MS-553 monotherapy, Cohort B will 
evaluate MS-553 in combination with acalabrutinib, and Cohort C will evaluate MS-553 in 
combination with venetoclax and rituximab. A schema of the three cohorts is shown in Figure 1 
in Section 3.1.4.  
 
 
 
 
3.1.1. 
Cohort A: MS-553 Monotherapy 
The first part of the study, the dose escalation portion (Cohort A1), will determine the safety 
of MS-553 and the MTD or initial RP2D. After the initial RP2D is identified, an expansion 
cohort (Cohort A2) will be started at the MTD or initial RP2D, enrolling approximately 25 
patients with relapsed/refractory CLL/SLL who (a) have failed at least two prior lines of 
therapies, or (b) have either acquired clinical and/or molecular resistance to a BTK inhibitor 
(i.e., BTK and/or PLCG2 mutation) after achieving initial response to a BTK inhibitor. In 
addition, an optional expansion cohort (Cohort A3) of approximately 25 patients with 
aggressive lymphoma, including Richter’s transformation, DLBCL and MCL will be enrolled. At 
least 15 patients in Cohort A3 shall be MCL patients. 
 
 
 
additional patients will be enrolled in Cohort A2 to further testing two dose levels before a 
final RP2D can be declared. 
The two dose schedules designated as dose schedule 1 (DS1) and dose schedule 2 (DS2) are as 
follows: 
Dose Schedule 1 (DS1) 
 
MS-553 Oral Daily Dose 
Week 1 
150 mg BID 
Week 2 
200 mg BID 
Week 3 and beyond 
250 mg BID 
 
Dose Schedule 2 (DS2) 

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MS-553 Oral Daily Dose 
Cycle 1 and beyond 
225 mg BID  
 
Twenty of the 40 additional patients will be treated with DS1 and the other 20 patients with 
DS2. The patients will be allocated to either DS1 or DS2 in alternating fashion, according to the 
sequence of their enrollment. For example, the first enrolled patient will be allocated to DS1, 
the second patient to DS2, the third patient to DS1, the fourth to DS2, and so on. 
The optional expansion cohort (Cohort A3) will be closed to accommodate the new dose 
testing plan. 
 
 
 
 
3.1.2. 
Cohort B: MS-553 in combination with acalabrutinib 
As soon as the MTD or initial RP2D is declared for MS-553 monotherapy in Cohort A1, MTD or 
initial RP2D of MS-553 in combination with acalabrutinib will be evaluated at an MS-553 
starting dose of 150 mg BID (Cohort B1) in treatment experienced CLL/SLL patients who are BTK 
inhibitor naïve, eligible for standard of care treatment with a BTK inhibitor as a single agent and 
who have had no more than 2 previous lines of treament regimens. The number of patients in 
the dose escalation will range from 3 to approximately 12 patients.  Once the MTD or initial 
RP2D is known for the combination (Cohort B1), two expansion cohorts (B2 and B3) will be 
started in parallel to further evaluate the safety of the combination regimens, as well as to 
assess early evidence of anti-tumor activity.  
One of the expansion cohorts (Cohort B2) will evaluate MS-553 in combination with 
acalabrutinib in treatment experienced CLL/SLL patients who are BTK inhibitor naïve, are 
eligible for treatment with a BTK inhibitor as a single agent, and who have had no more than 2 
previous lines of treatment regimens.  Approximately 20 patients will be enrolled in Cohort B2 
to further confirm the safety of the combination and assess early evidence of anti-tumor 
activity. 
The other expansion cohort (Cohort B3) will evaluate MS-553 in combination with 
acalabrutinib in treatment experienced CLL/SLL patients who have received no more than 2 
prior lines of therapy and have TP53 abnormalities (including 17p deletion and P53 
mutations). Once this cohort is open, all patients with TP53 abnormalities who are also naïve 
to BTK inhibitors will be preferentially enrolled in Cohort B3, rather than Expansion Cohorts 
B2. Approximately 10 treatment experienced CLL/SLL patients eligible for standard of care 
acalabrutinib will be enrolled in Cohort B3 to further confirm the safety of the combination 
and assess early evidence of anti-tumor activity. 

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3.1.3. 
Cohort C: MS-553 in combination with venetoclax and rituximab 
As soon as the MTD or initial RP2D is declared for MS-553 monotherapy in Cohort A1, MS-553 
will be evaluated in combination with venetoclax and rituximab at the starting dose of 150 mg 
BID in treatment experienced CLL/SLL patients who are eligible for standard of care venetoclax 
and rituximab, naïve to prior Bcl-2 inhibitor treatment, and have had no more than 2 prior 
lines of treatment regimens. The number of patients in dose escalation will range from 3 to 
approximately 12 patients per dose group.   
Once the MTD or initial RP2D is known for the combination (Cohort C1), one expansion cohort 
will be initiated to further evaluate the safety of the combination regimen, as well as to assess 
early evidence of anti-tumor activity. This cohort (Cohort C2) will enroll treatment 
experienced CLL/SLL patients who are eligible for standard of care venetoclax and rituximab, 
naïve to prior Bcl-2 inhibitor treatment and have had no more than 2 previous lines of 
treatment regimens.  Approximately 20 patients will be enrolled to further confirm the safety 
of the combination and assess early evidence of anti-tumor activity. 
3.1.4. 
Study Design Schematic 
 
Figure 1: 
Study Schema 
 
Figure 1:  Cohort schema including dose escalation for monotherapy and each of the combinations as well as 
subsequent expansion cohorts. 
3.2. 
Treatment Duration 
Patients will be treated in 28-day cycles. Patients on this study may continue receiving study drug 
provided they continue to tolerate the drug, have no evidence of progression, do not meet any of 
the criteria for discontinuation, and the patient’s participation in the study has not ended. The 
anticipated median treatment duration is unknown. Patients who have discontinued treatment for 
progressive disease and/or who have started another anticancer therapy or an investigational 
Cohort A1
Monotherapy MS-553
Initial RP2D identified
Expansion Cohort A2 at A1 
initial RP2D
R/R CLL who
•
Have failed at least two 
prior lines of therapies, or
•
Have acquired resistance 
to a BTK inhibitor after 
achieving initial response
Expansion Cohort A3 at A1 
initial RP2D
Aggressive lymphoma 
including Richter’s, DLBCL, MCL
Cohort B1
MS-553 + acalabrutinib dose escalation
Starting at least one dose lower of monotherapy initial RP2D
Treatment experienced CLL patients:
•
BTKi naive
•
Eligible for BTKi as SOC
•
No more than 2 prior lines of therapies
Expansion Cohort B2 at B1 RP2D
Treatment experienced CLL patients:
•
BTKi naive
•
Eligible for BTKi as SOC
•
No more than 2 prior lines of 
therapies
Expansion Cohort B3 at B1 RP2D
Treatment experienced CLL patients:
•
BTKi naive
•
Eligible for BTKi as SOC
•
No more than 2 prior lines of 
therapies
•
TP53 abnormalities
Cohort C1
MS-553 + venetoclax + rituximab dose 
escalation starting at least one dose 
lower of monotherapy initial RP2D
•
Treatment experienced with no 
more than 2 lines of prior therapies
•
Eligible for SOC of venetoclax and 
rituximab
•
Naïve to venetoclax
Expansion Cohort C2 at C1 RP2D
•
Treatment experienced with no 
more than 2 lines of prior 
therapies
•
Eligible for SOC of venetoclax and 
rituximab
•
Naïve to venetoclax

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product, will be followed for survival every 24 weeks (± 7 days) for up to 3 years (follow up can 
include clinic visit, chart review or telephone contact). Patients who discontinue treatment for 
reasons other than progressive disease, use of other anticancer therapy or an investigational 
product, death, or withdrawal of consent will have response assessments every 12 weeks (± 7 
days) until progressive disease occurs. Thereafter they will be followed for survival every 24 
weeks (± 7 days) for up to 3 years. All patients will discontinue survival follow-up upon 
completion of 3 years of survival follow-up, withdrawal of consent, death, or study closure, 
whichever is the earliest. The Sponsor reserves the right to discontinue the study for clinical or 
administrative reasons at any time.  
3.3. 
Dose Escalation  
3.3.1. 
Dose Escalation: Monotherapy Cohort A1 
The study will be conducted as a limited 3+3 phase 1 dose escalation study with potential 
expansion cohorts. Clinical safety data in healthy volunteers suggest very few toxicities at 100 to 
200 mg BID for up to 14 days. A conservative starting dose of 100 mg BID has been chosen and 
will be escalated according to Table 2 below: 
 
 
 
          Table 2:  Monotherapy Dose Levels 
Dose Level 
Dose  
0 
100 mg BID 
1 
200 mg BID 
2 
250 mg BID 
3 
300 mg BID 
4+ 
TBD* mg BID  
*To be determined 
A conventional “3+3” patient enrollment scheme will be followed during the dose escalation 
part of the study. Patients will be dosed with MS-553 by mouth twice daily at an initial dose 
(dose level 0, DL 0) of 100 mg BID daily in 28-day cycles. MS-553 shall be taken with food. 
At least 3 patients will be enrolled at starting dose level 0 (100 mg BID).  
After completion of dose level 3 (DL 3), the dose escalation of MS-553 can continue in 50 mg 
BID increments (or lower if an intermediate dose level is introduced) until a MTD is reached. 
Or the Safety Review Committee and/or the Sponsor may declare an initial RP2D, if further 
dose escalation is not warranted prior to reaching MTD.  Furthermore, in order to facilitate 
initial RP2D determination, select cohort(s) may be expanded up to 10 patients total in a 
cohort to assess the safety and clinical activity of MS-553 provided that the dose does not 
exceed the MTD and the Safety Review Committee, upon reviewing the safety and 
tolerability, efficacy, and available PK and PD data, agrees. The DLT period is defined in this 
cohort as 28 days. 

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3.3.2. 
Dose Escalation: General Principles 
During the first cycle of any dose level, if <1 Dose Limiting Toxicity (DLT; as defined in Section 
3.3.1) is observed, then escalation to the next higher dose level will follow the 3+3 design and 
proceed when 3/3 or 5/6 patients have completed one 28-day treatment cycle without a DLT. If 
at least 1 of 3 patients experiences a DLT at any dose level, the cohort will be expanded to 6 
patients. If ≥2/3 or ≥2/6 patients experience a DLT within a cohort, the MTD will be 
considered to have been exceeded. No additional patients will be enrolled in that cohort, and 
dose escalation will stop. If at least 3/3 or 5/6 patients have completed one 28-day treatment 
cycle at the previous dose level without a DLT, then that dose may be accepted as the MTD. At 
Sponsor discretion, additional patients may be enrolled into a cohort where < 1 patient 
develops a DLT to obtain additional safety, PK, PD, and/or efficacy data.  
If greater than 1/6 patients experience a DLT at DL 1, then 100 mg BID may be declared the 
MTD, or intermediate dose levels and/or alternative dosing schedules may be evaluated upon 
review of safety, pharmacodynamic, and/or pharmacokinetic data.  
Each dose cohort will include a minimum of 3 evaluable patients for assessment of DLT. In order 
to be considered evaluable for DLT, patients must meet at least one of the following criteria:  
1. Complete dosing through the DLT period and receive at least 75% of MS-553 intended 
doses during that time.  
2. Discontinue due to DLT  
During the DLT evaluation period, non-evaluable patients who discontinue treatment before 
DLT period is complete for reasons other than study drug-related toxicities (e.g., progressive 
disease, non-compliance) or do not receive at least 75% of MS-553 intended doses in the DLT 
period, will be replaced with additional patients enrolled at the same dose level. After a DLT is 
resolved or reversed to  Grade 1, the study drug may continue at a lower dose level at the 
discretion of the investigator. 
A decision on dose escalation to the next dose level will be based on evaluation of the 
clinical safety profile, and may also include evaluation of MS-553 exposure in treated 
patients. After the completion of each cohort, safety and PK data (if available) will be 
reviewed and a decision on dose escalation will be determined by the Investigators, the 
Safety Review Committee, and the Sponsor.  
During the dose escalation period, intra-patient dose escalation will be permitted at the 
discretion of the study investigators and after discussion with the medical monitor and 
Sponsor, if the following criteria are met: 
1. The next higher dose for the regimen has been demonstrated to be safe by at least 
3 patients and all 3 patients complete one cycle without experiencing any DLT. 
2. The patient who will receive the higher dose has not experienced a DLT and has also 
completed at least 2 cycles of therapy at their current dose. 
3. Intra-patient dose escalation can be increased only by one dose level at a time.  

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After completion of each dose escalation cohort, the SRC will review clinical safety and 
tolerability data as well as PK data available at the time for all patients in order to confirm 
dose escalation to the next dose level. Based on the SRC’s review, a cohort may be expanded, 
or an intermediate or a lower-than-planned dose level or an alternative dosing schedule may 
be evaluated.  
All patients will continue to be monitored for approximately 30 days ( +7days)  after 
treatment discontinuation for development of any late treatment emergent adverse events. 
Any such late adverse events will be considered in the determination of the MTD/initial RP2D 
and dose schedule to be taken forward into the expansion phase of the study.  
 While the 
MTD was not reached, an initial RP2D was identified. 
 the SRC reviewed all 
available efficacy, safety, PK and PD data of Cohort A1 and some Cohort A2 patients and 
agreed on an updated RP2D with a ramp-up dosage regimen as shown in Table 3 below. 
Table 3:  
Initial Recommended Phase 2 Dose 
MS-553 
Oral Daily Dose 
Weeks 1 to 4 (Cycle 1) 
150 mg BID 
Weeks 5-8 (Cycle 2) 
200 mg BID  
Weeks 9 and beyond 
(Cycle 3+) 
250 mg BID 
 
 
 
 
 
 
The two new dose schedules are designated as dose schedule 1 (DS1) and dose schedule 2 
(DS2) are as follows: 
Dose Schedule 1 (DS1) 
 
MS-553 Oral Daily Dose 
Week 1 
150 mg BID 
Week 2 
200 mg BID 
Week 3 and beyond 
250 mg BID 
 
Dose Schedule 2 (DS2) 
 
MS-553 Oral Daily Dose 
Cycle 1 and beyond 
225 mg BID  
 

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Twenty of the 40 additional patients will be treated with DS1 and the other 20 patients with 
DS2. The patients will be allocated to either DS1 or DS2 in alternating fashion, according to the 
sequence of their enrollment. For example, the first enrolled patient will be allocated to DS1, 
the second patient to DS2, the third patient to DS1, the fourth to DS2, and so on. 
The optional expansion cohort (Cohort A3) will be closed to accommodate the new dose 
testing plan. 
3.3.3. 
Dose Escalation: Combination Cohort B1  
 
Once the MTD or initial RP2D is established for the monotherapy Cohort A1, enrollment in 
Cohort B1 will begin at 150 mg BID. The principles outlined in Section 3.3.2 will be followed for 
the Dose Escalation for the combination regimens.  One or more 3+3 dose escalation cycles 
may be undertaken to determine an initial RP2D for MS-553 for each combination expansion 
cohorts. At least two dose levels are planned for Cohort B1. The dosing schedule and DLT 
evaluation periods are described in the table below. 
Table 4:  
Dosing Schedule and DLT Evaluation Periods for Cohort B1 
MS-553 Dose Levels 
Dosing Schedule 
DLT Evaluation Period 
150 mg BID 
In 28-day cycles 
Cycle 1 
200 mg BID 
In 28-day cycles 
Cycle 1 
MTD determination, DLT and safety assessments will follow the general principles outlined in 
Cohort A1. Initial RP2D of MS-553 in the combination therapy is expected to be reached 
before the dose escalation reaches 250 mg BID, the high dose level of MS-553 monotherapy 
under testing. Intermediate dose levels (i.e. at 25 mg increment) or lower dose levels may 
also be evaluated as determined by the Investigators, the SRC and Sponsor until the MTD or 
initial RP2D for the combination is determined.  
3.3.4. 
Dose Escalation: Combination Cohort C1  
Once the MTD or initial RP2D is established for the monotherapy (Cohort A1), enrollment in 
Cohort C1 will begin. Starting with cycle 1, patients will be dosed with MS-553 by mouth twice 
daily beginning at 150 mg BID, and rituximab will be started according to standard of care, and 
as outlined in Section 5.2.3.3. Beginning with Cycle 3, venetoclax dosing once daily by mouth 
will be initiated with weekly dose escalations from 20 mg to 400 mg over 5 weeks.  
At least two dose levels are planned for Cohort C1. The dosing schedule and DLT evaluation 
periods are described in the table below. 
Table 5:  
Dosing Schedule and DLT Evaluation Periods for Cohort C1 
Dose 
level 
Cycle 1 
Cycle 2 
Cycle 3 
Cycle 4+ 
DLT 
Period(s) 
150 mg 
BID 
150 mg 
BID 
150 mg 
BID 
150 mg 
BID 
150 mg 
BID 
Cycle 1 
through 4 
200 mg 
BID 
200 mg 
BID 
200 mg 
BID 
200 mg 
BID 
200 mg 
BID 
Cycle 1 
through 4 

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MTD determination, DLT and safety assessments will follow the general principles outlined in 
Cohort A1.  Initial RP2D of MS-553 in the combination therapy is expected to be reached before 
or when the dose escalation reaches 250 mg BID, the high dose level of MS-553 under testing 
as monotherapy. Intermediate dose levels (i.e., at 25 mg increment) or lower dose levels may 
also be evaluated as determined by the Investigators, the Safety Review Committee and 
Sponsor until the MTD or initial RP2D for the combination is determined.  
3.3.5. 
Dose Limiting Toxicity Definition 
 
Dose limiting toxicities (DLT) occurring during DLT period appropriate for the cohort will be used 
to determine which dose of MS-553 will be examined in expansion cohorts. Toxicities will be 
assessed utilizing the National Cancer Institute Common Terminology Criteria for Adverse 
Events (NCI CTCAE v5.0) in Appendix 4. 
Dose limiting toxicities (DLT) are defined as any of the following treatment-emergent events 
occurring from Cycle 1 Day 1 (after administration of MS-553) through the end of the DLT period 
(unless the event is determined to be unrelated to the drug): 
1. Death 
2. Hematologic toxicities: 
• Grade 4 neutropenia for ≥ 7 days  
• Grade 3 febrile neutropenia: absolute neutrophil count (ANC) <1000/mm3 and single 
temperature >38.3°C (101°F) or a sustained temperature ≥38°C (100.4°F) for > 1 
hour 
• Grade 4 thrombocytopenia ≥ 14 days (patients with baseline platelet count of 
≥ 50 x 109/L)  
• Grade 4 thrombocytopenia ≥ 28 days (patients with baseline platelet count < 50 x 
109/L) 
• ≥ Grade 3 thrombocytopenia associated with ≥ Grade 2 hemorrhage 
• New ≥ Grade 3 anemia requiring transfusion in a patient previously transfusion-
independent. 
3. Nonhematologic toxicities: 
• Any other ≥ Grade 3 toxicity not reversed to any one of the following three 
conditions in 7 days with appropriate intervention: a) baseline; b) < Grade 1; or c) a 
status considered to be controlled by the Safety Review Committee (For example, 
isolated ≥ Grade 3 laboratory toxicity [e.g., electrolyte abnormalities] not associated 
with clinical signs or symptoms and is reversed to Grade 2 with appropriate 
intervention in 7 days is not considered a DLT.) 
• Any treatment-related adverse event (TEAE) requiring >25% of doses of scheduled 
study drug to be withheld during  the DLT period.  

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All patients will continue to be monitored for approximately 30 days ( +7days)  after 
treatment discontinuation for development of any late treatment emergent adverse events. 
Any such late adverse events will be considered in the determination of the MTD/initial RP2D 
and dose schedule to be taken forward into the expansion phase of the study. 
3.4. 
Dose Expansion Cohorts 
Dose expansion cohorts will be initiated after determination of the MTD or initial RP2D for 
monotherapy or combination treatment.  
3.4.1. 
Expansion Cohort A2: Monotherapy 
A dose expansion cohort (“expansion cohort A2”) of approximately 25 patients with refractory 
or relapsed CLL/SLL who (a) have failed at least two prior lines of therapies, or (b) have either 
acquired clinical and/or molecular (e.g., BTK and/or PLCG2 mutation) resistance to a BTK 
inhibitor after achieving an initial response will be activated, and enrolled at the initial RP2D or 
the highest dose level at which fewer than 2/6 patients have completed the first cycle without 
experiencing DLT (e.g., the MTD). 
 
 
 an 
additional 40 patients will be enrolled in Cohort A2 to further testing two dose levels before a 
final RP2D can be declared.  
3.4.1.1. 
Optional Expansion Cohort A3: Monotherapy 
In addition, an optional expansion cohort of approximately 25 patients with aggressive 
lymphoma, including Richter’s Transformation, DLBCL and MCL, can be enrolled simultaneously 
once activated. At least 15 patients in this cohort shall be MCL patients.   
The optional expansion cohort A3 will be closed to accommodate testing two dose schedules in 
Cohort A2.  
3.4.2. 
Expansion Cohorts B: Combination with Acalabrutinib 
 
  
3.4.2.1. 
Expansion Cohort B2 
Once the MTD/initial RP2D is established in Cohort B1 for MS-553 in combination with 
acalabrutinib, an expansion cohort will be opened. This cohort will evaluate MS-553 in 
combination with acalabrutinib in treatment experienced CLL/SLL patients who are BTK 
inhibitor naïve, are eligible for standard of care treatment with a BTK inhibitor as a single agent, 
and who have had no more than 2 previous lines of treatment regimens.  Approximately 20 
patients will be enrolled in Cohort B2 to further confirm the safety of the combination and 
assess early evidence of anti-tumor activity.  

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3.4.2.2. 
Expansion Cohort B3 
Once the MTD/initial RP2D is established in Cohort B1, Cohort B3 will be opened in parallel to 
Cohort B2 to evaluate MS-553 in combination with acalabrutinib in treatment experienced  
CLL/SLL patients who are BTK inhibitor naïve, have TP53 abnormalities (including 17p deletion 
and P53 mutations), and have had no more than 2 previous lines of therapy. Once this cohort is 
open, all patients with TP53 abnormalities who are also naïve to BTK inhibitors will be 
preferentially enrolled in Cohort B3, rather than Expansion Cohorts B2. Approximately 10 
treatment experienced CLL/SLL patients eligible for standard of care acalabrutinib will be 
enrolled in Cohort B3 to further confirm the safety of the combination and assess early 
evidence of anti-tumor activity. 
3.4.3. 
Expansion Cohorts C: Combination of Venetoclax and Rituximab 
 
  
3.4.3.1. 
Expansion Cohort C2 
Once the MTD or initial RP2D is established in Cohort C1 for the combination of venetoclax, 
rituximab and MS-553, an expansion Cohort C2 will be initiated. Treatment experienced CLL/SLL 
patients who are eligible for standard of care venetoclax and rituximab, naïve to prior Bcl-2 
inhibitor treatment and have had no more than 2 previous lines of treatment regimens can be 
enrolled. Approximately 20 patients will be enrolled to further confirm the safety of the 
combination and assess early evidence of anti-tumor activity. 
3.5. 
Safety Review Committee 
The SRC will monitor the safety of all patients in the dose escalation segment. The Committee 
will receive all available demographics, PK, and safety data (including, but not limited to, lab and 
ECG results, physical exam findings, vital signs, adverse events) for all patients dosed within a 
given cohort in order to make a decision regarding dose escalation to the next dose level. Based 
on the SRC’s review, a dose escalation cohort may be expanded or an intermediate or a lower-
than-planned dose level may be evaluated. The SRC will include the Sponsor representatives, 
the medical monitor, and Principal Investigators.  
A separate Safety Management Plan outlines the roles and responsibilities for those involved in 
the collection and processing of Serious Adverse Events (SAEs), and pregnancies for this clinical 
study. 
3.6. 
Remaining on Treatment 
Patients will remain on treatment with MS-553 until disease progression, initiation of other 
anti-cancer therapy or investigational drug product, patient refusal of treatment or revocation 
of consent, or death due to any cause.  

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3.7. 
Response Assessment 
Response data will be collected at screening and on day 1 of every cycle beginning with the first 
treatment visit and ending at the end of treatment visit, which should occur 30 (+ 7 days) after 
the last dose of study drug, if the condition of the patient permits, or up to the start of other 
anti-cancer therapy or investigational drug product(s), whichever is sooner. Patients who 
discontinue treatment for reasons other than progressive disease, use of other anticancer 
therapy or an investigational drug product, death, or withdrawal of consent will undergo 
response assessments every 12 weeks (± 7 days) until progressive disease occurs.  
Response data consists of a combination of interviews, physical exams, labs, and scans. Not all 
labs and scans will be obtained at each time point. See Section 6.2 and Appendix 1 for complete 
details. 

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4. 
PATIENT POPULATION 
CLL is most commonly a disease of the elderly, with a median age at diagnosis of 71, but disease 
has been witnessed in adults of all ages. This study is targeted at CLL adults of any age who meet 
the inclusion criteria for the cohort to which the patient is enrolled. 
 
 
 
 
4.1. 
Inclusion Criteria 
To be eligible for inclusion in the dose escalation cohorts or expansion cohorts in this study, 
patients must meet all of the following criteria: 
1. Age 18 years or older. 
2. Diagnosis of CLL or SLL: 
a. History of histologically documented CLL or SLL that meets IWCLL diagnostic 
criteria according to the 2008 guidelines, and 
b. Indication for treatment as defined by the 2008 IWCLL guidelines, or the need 
for disease reduction prior to allogeneic transplantation. 
3. Cohort specific inclusion requirements: 
Cohort A1 (MS-553 monotherapy) 
Relapsed after, or is refractory to, progressing after at least two prior lines of therapies 
with the following exceptions: 
a. Patients with a 17p deletion who have already received venetoclax; or 
b. Patients who have TP53 mutation (prior therapy is not required) which shall be 
confirmed 
 at screening for enrollment. 
 
 
 
or 
c. Patients with clinical and/ or molecular (e.g., BTK and/or PLCG2 mutation) 
resistance after achieving initial response to a BTK inhibitor.  
• 
For patients without clinical resistance, molecular resistance (i.e., BTK and/or 
PLCG2 mutation) shall be confirmed 
 at screening for 
enrollment. 
Cohort A2 (MS-553 monotherapy) 
Relapsed/refractory CLL/SLL patients who have:  
a. Failed at least two prior lines of therapies, or 

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b. Developed clinical and/or molecular (i.e., BTK and/or PLCG2 mutation) resistance 
after achieving an initial response to a BTK inhibitor  
• 
For patients without clinical resistance, molecular resistance (i.e., BTK 
and/or PLCG2 mutation) shall be confirmed 
 at 
screening for enrollment. 
Cohort A3 (MS-553 monotherapy) See Inclusion Criterion #4 below 
Cohort B1, B2 (MS-553 in combination with acalabrutinib) 
Treatment experienced patients with no more than two prior lines of therapies, who 
a. Are naïve to prior BTK inhibitor treatments, and  
b. Are eligible for single agent BTK inhibitor 
Cohort B3 (MS-553 in combination with acalabrutinib) 
Treatment experienced patients with no more than two prior lines of therapies, who 
a. Are naïve to prior BTK inhibitor treatments, and 
b. Are eligible for single agent BTK inhibitor, and 
c. Have 17p deletion or TP53 mutation which shall be confirmed 
 at 
screening for enrollment. 
 
 
Cohort C1, C2 (triplet regimen of MS-553 + rituximab + venetoclax) 
Treatment experienced patients receiving no more than 2 prior lines of treatment 
regimens, who 
a. 
Are naïve to prior Bcl-2 inhibitor treatments, and 
b. 
Are eligible for venetoclax in combination with rituximab.  
4. For inclusion in the Cohort A3, patients must meet both of the following diagnostic 
criteria instead of inclusion criterion #2or #3: 
a. Diagnosis of aggressive lymphoma, explicitly comprising Richter’s 
Transformation of CLL/SLL to Diffuse Large B-Cell Lymphoma (DLBCL); 
Activated B-cell (ABC), or non-GCB DLBCL not transformed from CLL/SLL, or 
MCL. Other histologies may be included after discussion with and approval of 
the study principal investigator and Sponsor medical monitor. 
b. Additionally, patients with MCL or de novo ABC or non-GCB subtype DLBCL 
patients must have received at least two lines of prior therapy and MCL patients 
must have failed at least a BTK inhibitor. Patients with Richter’s transformation 
have no such restriction on the use of prior therapy. 
5. WHO/ECOG performance status ≤ 2. 

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6. Meet laboratory parameters (see Exclusion Criteria). 
7. In the opinion of the investigator, all of the following criteria are met:  
a. Have life expectancy ≥ 2 months  
b. Are willing to undergo all study-related evaluations and procedures, and 
c. Have ability to understand and willingness to execute a written informed consent 
document. 
4.2. 
Exclusion Criteria 
Patients who meet any of the following criteria are not eligible for this study. 
1. 
Current transformation of CLL/SLL to PLL, non-Hodgkin lymphoma, or Hodgkin 
lymphoma. 
d. Patients being considered for inclusion in the optional expansion cohort 
(aggressive lymphoma) are not excluded on the basis of transformed lymphoma 
but must meet the definition of aggressive lymphoma outlined in the inclusion 
criteria for Cohort A3. 
2. 
Active and uncontrolled autoimmune cytopenia(s). 
3. 
Any of the following prior therapies within 14 days prior to Cycle 1 Day 1: 
a. Major surgery 
b. Corticosteroids greater than 20 mg / day prednisone (or equivalent), unless used 
by inhalation or topical route, or unless necessary for premedication before and/ 
or after iodinated contrast dye. Higher doses, and more prolonged courses of 
steroids are permitted under certain circumstances after discussion with the 
medical monitor and/or Sponsor including, but not limited to, the following: 
i. 
Treatment of disease-related autoimmune hemolysis or autoimmune 
thrombocytopenia; 
ii. 
Symptomatic relief of lymphadenopathy – preferably after completion 
of Cycle 1;  
iii. 
Symptomatic relief of B symptoms – after completion of Cycle 1; 
iv. 
Treatment of GVHD – please discuss with medical monitor; 
v. 
Short courses (up to 14 days) if needed for any intercurrent condition 
such as acute exacerbation of any non-disease-related condition (e.g., 
arthritis, asthma, COPD) including steroid dose modification required 
for adrenal insufficiency. 
c. Cytotoxic chemotherapy or biologic therapy, with the exception of BCR pathway 
kinase inhibitors for which only a 24-hour wash out prior to Cycle 1 Day 1 is 
required. 
4. Failure to recover:  

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a. From toxicity to Grade 1 or less from prior chemo- or radiotherapy or biologic 
therapy with the exception of alopecia, atrial fibrillation or hypertension that 
developed during treatment with a BTK inhibitor.  
b. To Grade 2 or less for atrial fibrillation or hypertension that occurred as a 
consequence of treatment with a BTK inhibitor. 
5. CNS leukemia/lymphoma – including history of treated CNS disease even if the patient is 
asymptomatic. 
6. Inadequate bone marrow function / hematopoietic reserve, except in the case of 
documented bone-marrow involvement, evidenced as: 
a. 
Absolute Neutrophil Count (ANC) < 1 x 109/L 
b. 
Platelet count < 30 x 109/L 
7. Inadequate liver or renal function as specified below: 
a. 
Serum total bilirubin > 2 x ULN (upper limit of normal), or > 3 x ULN in case of liver 
involvement with CLL/SLL, or serum total bilirubin > 3 x ULN in patients with 
documented history of Gilbert’s disease and normal direct bilirubin. 
b. 
AST or ALT > 2.5 x ULN, or > 5 x ULN if CLL/SLL is present in the liver.  
c. 
Estimated GFR < 30 mL/min. 
8. For Cohort B patients, active treatment with strong CYP3A inhibitors, CYP3A inducers or 
proton pump inhibitors, unless medication can be stopped prior to enrollment in the 
study.  
9. For Cohort C patients, active treatment with strong and moderate CYP3A inhibitors, 
strong CYP3A inducers, or strong P-gp inhibitors unless medication can be stopped prior 
to enrollment in the study.  
 
 
 
 
 
 
 
 
12. Receiving treatment with medications that are known to be strong inducers, inhibitors or 
sensitive substrates of CYP3A4/5 or medications with risk for QT prolongation that 
cannot be discontinued prior to study entry (at least 48 hours prior to the start of Cycle 
Day 1 dosing). For any exceptions, discussion with medical monitor and/or Sponsor is 
needed. Weak or moderate inhibitors may be used with caution, except as otherwise 
specified in Exclusion Criteria. Examples of these medications are listed in Appendix 3. 
13. Clinically significant cardiac diseases, including any of the following: 
a. History or presence of ventricular tachyarrhythmia 

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b. Presence of unstable/uncontrolled atrial fibrillation is exclusionary. Patients with 
stable atrial fibrillation (rate controlled pharmacologically) are eligible provided that 
they do not meet any other exclusion criteria. Patients whose atrial fibrillation 
occurred as a consequence of treatment with a BTK inhibitor may be eligible for the 
study provided that the patient is asymptomatic and on a stable dose of medication 
for rate control, and only after discussion with the medical monitor. 
c. Unstable angina pectoris or acute myocardial infarction within 3 months prior to start 
of study drug. 
d. NYHA functional class III or IV heart failure 
e. Baseline QTcF interval of > 480 msec. Prior QTc prolongation due to prior medication 
no longer on board is not exclusionary. A family history of QTc interval prolongation 
by itself is not exclusionary provided that the patient’s QTc is not prolonged.  For 
those with prolonged QTc interval due to bundle branch block, patient may be 
eligible after documentation of a “corrected value” ≤480 msec and confirmation by 
cardiologist that patient is asymptomatic and that no other cardiac conduction or 
cardiac abnormality is present that would pose any safety issue for the patient.  
Note: As the eligibility criteria specify the QT interval corrected by the method of 
Fridericia (QTcF), any correction for widened QRS should be sure to include a cube-
root corrected R-R interval. Please discuss/consult with a cardiologist if there are any 
questions. 
14. History of another malignancy that in the opinion of the investigator limits survival to less 
than 2 years. 
 
 
 
 
 
17. Gastrointestinal dysfunction, including motility or malabsorption syndromes or 
inflammatory bowel disease which could limit absorption of MS-553. 
18. Known HIV positivity, or active hepatitis B or C infection with detectible viral nucleic acid 
in the blood. 
19. Severe systemic infections requiring intravenous antibiotics within the two weeks prior 
to initiation of MS-553. 
20. Any other life-threatening illness, medical condition, or laboratory abnormalities or 
therapies that in the opinion of the investigator could compromise the safety of the 
patient or interfere with analysis of study endpoints.  
21. Women who are lactating or pregnant. 
22. Women of child-bearing potential or male partners of women of child-bearing potential 
who will not agree to use highly effective method of contraception throughout the entire 

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study period and for a minimum of 3 months after the last dose of study drug. Highly 
effective contraception methods include: 
a. Total abstinence or 
b. Male or female sterilization or 
c. Combination of any two of the following: 
i. Use of oral, injected or implanted hormonal methods of contraception 
ii. Placement of an intrauterine device (IUD) or intrauterine system (IUS) 
iii. Barrier methods of contraception: condom or occlusive cap (diaphragm or 
cervical/vault caps) with spermicidal foam/gel/film/cream/vaginal 
suppository. 
Women are considered post-menopausal and not of child-bearing potential if 
they have had 12 months of natural (spontaneous) amenorrhea with an 
appropriate clinical profile (age appropriate, history of vasomotor symptoms) or 
have had surgical bilateral oophorectomy (with or without hysterectomy) or tubal 
ligation at least six weeks ago. In the case of oophorectomy alone, only when the 
reproductive status of the woman has been confirmed by follow up hormone 
level assessment is she considered not of childbearing potential. 
Women of child-bearing potential who will not agree to the following are 
ineligible for the study: 
• Refrain from breastfeeding and donating oocytes or blood during the 
course of the study and for 3 months after the last dose of study 
medication, and 
• Ongoing pregnancy testing during the course of the study and at the end 
of treatment visit.  
23. Men who will not agree to the following are ineligible for the study: 
• Refrain from donation of sperm or blood during the course of the study and 
for 3 months after the last dose of study medication, and 
• Completely abstain from sexual contact, or must always use a latex or 
synthetic condom during any sexual contact until 3 months after the last dose 
of study drug, or have prior documentation of surgical sterilization.  

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5. 
STUDY TREATMENT 
5.1. 
Overall Study Design Investigational Study Drug 
Detailed information about MS-553 is available in the IB and the Pharmacy Manual.  
5.1.1. 
MS-553 Treatment Description  
MS-553 drug product is supplied as an immediate release, round, white film-coated tablet 
provided in three strengths: 25 mg, 50 mg and 100 mg. The precise composition of the drug 
product and purpose of all ingredients are provided in the IB.  
5.1.1.1. 
 Storage and Handling 
MS-553 drug product drug product is packaged in white, opaque, high-density polyethylene 
bottles with white, opaque, polypropylene, child resistant caps. Counts of 30 tablets each for 
25 mg, 50 mg and 100 mg strength tablets are provided. Each bottle will be labeled with its 
contents, product lot number, required storage conditions, Sponsor’s address, and any 
specific cautionary statement such as “New Drug – Limited by Federal Law to Investigational 
Use.” Bottles are to be stored at room temperature (15-30°C). Until dispensed to the patients, 
MS-553 drug product bottles should be stored in a securely locked area accessible only to 
authorized site personnel. The MS-553 drug product should not be repackaged into 
alternative containers other than the bottles in which it was supplied. Refer to the Pharmacy 
Manual for more specific storage and handling information. 
5.1.1.2. 
Study Drug Disposal 
Used (empty) bottles and opened bottles with unused study drug supplies will be destroyed 
on-site by the Investigational Pharmacy Service according to the site SOP after completion of 
study drug accountability by the study monitor. Study drug destruction will be documented in 
the study’s pharmacy binder. Unopened bottles will be returned to MingSight 
Pharmaceuticals Inc. or destroyed per the site SOP.  
5.1.2. 
Combination Treatment Description  
Combination treatment with either acalabrutinib (B Series Cohorts) or venetoclax and rituximab 
(C Series Cohorts) will be according to standard of care and as described in Section 5.2.3.2 and 
5.2.3.3, respectively.  The combination drugs should be stored, handled and disposed of 
according to the Complete Prescribing Information and site standard procedures for these 
drugs.  
5.2. 
Treatment Plan 
5.2.1. 
Premedication 
Appropriate anti-emetic drugs per institutional standard should be administered upon initiation 
of MS-553. 
 

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5.2.2. 
Administration Instructions 
MS-553 should be taken orally with food at approximately the same time each day. Patients 
should be instructed not to bite, chew or crush the tablet. If the patient is unable to take the 
entire tablet, contact the medical monitor for further instructions.  Refer to the Pharmacy 
Manual for more specific dose administration details. 
5.2.3. 
Dosing Instructions and Schedule 
5.2.3.1. 
MS-553 Dosing – All Cohorts 
MS-553 should be taken orally twice daily, every 12 hours ± 2 hours.  At C1D1, C1D8, and C2D1, 
the morning dose will be administered by clinic staff.  All other doses will be administered by the 
patient.  MS-553 should be taken at approximately the same time each day. Small variations in 
the twelve-hour schedule are understandable, but patients should be advised to adhere to the 
prescribed schedule as closely as possible. Adherence to the dosing schedule will be 
documented in the patient chart and EDC /electronic CRF at each scheduled visit.  Patients will 
record all at-home dosing in a patient diary. Refer to the Pharmacy Manual for more specific 
dose administration details.  
Administration Instructions: Dosing Interruptions, Missed Doses, and Vomited Doses 
If a dose is late but within 4 hours of its scheduled administration time it should be taken; if 
more than 4 hours have passed, the dose should be omitted and the patient should continue 
treatment with the next scheduled dose with no alteration of timing for the subsequent dose. 
The missed dose should be documented in the patient’s diary, chart and the EDC / electronic 
CRF. 
If vomiting occurs while taking MS-553, no re-dosing is allowed before the next scheduled 
dose. Treating physicians are encouraged to use prophylactic antiemetics per standard of care 
at their institution. 
Patients should be instructed that if they miss two or more doses consecutively that they 
must contact the investigator or other study site personnel. 
5.2.3.2. 
Acalabrutinib Dosing – B Cohorts  
For patients who are enrolled into any of the B series cohorts, acalabrutinib will be 
administered according to the Full Prescribing Information in combination with MS-553. As 
both acalabrutinib and MS-553 are substrates of P-glycoprotein (P-gp), it is recommended 
that acalabrutinib be taken 90 minutes (but at least 1 hour) prior to the time when MS-553 is 
taken to avoid potential drug-drug interaction. 
5.2.3.3. 
Venetoclax and Rituximab Dosing – C Cohorts 
For patients are enrolled into any of the C series cohorts, venetoclax will be initiated after 
eight weeks of treatment with MS-553 and rituximab. Rituximab will be administered 
according to the Full Prescribing Information except the necessary changes to allow for the 
dosing sequence of the triple drug combination regimen. In order to reduce the risk of TLS, 

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venetoclax will not be administered until Cycle 3. Beginning in Cycle 3, venetoclax will be 
started and dose escalated in accordance with the Prescribing Information from 20 mg to 400 
mg over five (5) weeks and administered in combination with rituximab and MS-533. 
Venetoclax should be taken with food ≥ 6 hr after the morning dose of MS-553. Venetoclax 
shall be administered in accordance with the Full Prescribing Information. 
For patients who have achieved complete responses with the treatment of the triplet 
regimen of MS-553, venetoclax, and rituximab, assessments of uMRD in a central lab will be 
performed in a fashion consistent with IWCLL 2018 guideline. If uMRD in both peripheral 
blood and bone marrow is confirmed in a central lab, MS-553 may be discontinued at the 
discretion of the investigators after completion of venetoclax administration as defined in 
package label.  
5.2.4. 
Concomitant and Supportive Therapy 
5.2.4.1. 
MS-553 
Treating physicians are encouraged to use prophylactic antiemetics per standard of care at their 
institution. 
Steroids in doses of up to 20 mg prednisone (or equivalent) daily are allowed for symptomatic 
relief of lymphadenopathy and B symptoms if needed, with preference for their 
administration to be delayed until after completion of Cycle 1. If steroids are needed to 
control lymphadenopathy-related issues, please discuss with the medical monitor/Sponsor. 
After completion of Cycle 1, higher dose steroids may only be used for therapeutic treatment 
of (1) GVHD or (2) autoimmune cytopenia after discussion with the medical monitor and/or 
Sponsor. Treatment with short courses of steroids is permitted for intercurrent illness as per 
standard of care practice.  
No other direct anti-leukemia therapy is permitted. 
Supportive care with blood products (prophylactic or therapeutic) and hematopoietic growth 
factors may be used in accordance with institutional standards. Use of myeloid or erythroid growth 
factor during DLT period should be discussed with the medical monitor and the study Sponsor. 
5.2.4.2. 
Acalabrutinib  
Treating physicians are encouraged to use concomitant and supportive therapy for 
acalabrutinib per standard of care at their institution.   
5.2.4.3. 
Venetoclax 
Prior to initiation of venetoclax, MS-553 and rituximab combination shall be given for two 
cycles to allow for debulking of tumor mass with the intent of avoiding tumor lysis syndrome 
(TLS). Venetoclax shall start on Cycle 3. Treating physicians should assess patient-specific 
factors for level of risk of TLS and provide prophylactic hydration and anti-hyperuricemics to 
patients prior to the first dose of venetoclax to reduce risk of TLS according to standard site 
procedures. After two cycles treatment of MS-553 and rituximab, restaging prior to Cycle 3 at 

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investigator discretion is allowed for patients who were initially high risk for TLS, to allow 
outpatient treatment if they now are low or medium risk. 
Table 6:  
Recommended TLS Prophylaxis Based on Tumor Burden in Patients with 
CLL/SLL 
Tumor Burden 
Prophylaxis 
Blood Chemistry Monitoringcde 
 
Hydrationa 
Anti-
hyperuricemics 
Frequency of Assessments 
All LN < 5cm 
AND  
ALC < 25 x 109/L 
Oral 
(1.5-2 L) 
Allopurinolb 
• First dose of 20 mg and 50 
mg: Pre-dose, 6 to 8 hours, 
24 hours 
• For subsequent ramp-up 
doses: Pre-dose 
Any LN ≥ 5cm 
to < 10cm 
OR 
ALC ≥25 x 109/L 
Oral 
(1.5-2 L) 
and consider 
additional 
intravenous 
Allopurinol 
• First dose of 20 mg and 50 
mg: Pre-dose, 6 to 8 hours, 
24 hours 
• For subsequent ramp-up 
doses: Pre-dose 
• For first dose of 20mg and 
50mg: Consider 
hospitalization for patients 
with CrCl < 80 mL/min 
LN ≥ 10cm 
OR  
ALC ≥ 25 x 
109/L 
AND any LN ≥ 5 
cm 
Oral 
(1.5-2 L) 
and intravenous 
(150-200 mL/hr 
as tolerated) 
Allopurinol; 
consider 
rasburicase if 
baseline uric acid 
is elevated 
• In hospital first dose of 20 
mg and 50 mg: Pre-dose, 4, 
8, 12, 24 hours 
• For subsequent ramp-up 
doses, outpatient: Pre-dose, 
6 to 8 hours, 24 hours 
a. Administer intravenous hydration for any patient who cannot tolerate oral hydration 
b. Start allopurinol or xanthine oxidase inhibitor 2 to 3 days prior to initiation of venetoclax 
c. Evaluate blood chemistries (potassium, uric acid, phosphorus, calcium, and creatinine); review in real time. 
d. For patients at risk of TLS, monitor blood chemistries at 6 to 8 hours and at 24 hours at each subsequent 
ramp-up dose or according to institutional standard. 
e. A window of one hour is permitted for the post-dose labs that are due <24 hours and a two-hour window is 
permitted for the 24-hour post dose labs. 
To reduce risks associated with neutropenia, granulocyte-colony stimulating factor (G-CSF) may 
be administered, if clinically indicated. 
5.2.4.4. 
Anti-CD20 mAb  
Consistent with the Prescribing Information of venetoclax, the anti-CD20 mAb used in the 
combination shall be rituximab for the relapsed/refractory CLL/SLL patients. Premedicate with 
acetaminophen and an antihistamine before each rituximab infusion according to institutional 
standard of care. Depending on the severity of any infusion-related reaction and the required 

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interventions, temporarily or permanently discontinue rituximab. Resume infusion according to 
institutional standard of care after symptoms have resolved.  
Provide prophylaxis treatment for Pneumocystis jirovecii pneumonia (PCP) and herpes virus 
infections for patients with CLL during treatment and for up to 12 months following treatment 
as appropriate.  
5.2.5. 
Excluded Medications 
Co-administration of strong (≥ 5-fold) inhibitors or inducers and sensitive substrates of 
CYP3A4/5 is prohibited and should be discontinued at least 48 hours prior to initiation of study 
drug. Medications with QT prolongation risk as listed in their package inserts or belong to drugs 
with known risk of Torsades de Pointers on crediblemeds.org should be avoided whenever 
possible. Refer to Appendix 3 for examples of these medications. When substitution of another 
medication is not feasible, discussion with the medical monitor and Sponsor will be needed. For 
example, the metabolism of the drug in question may be more affected by other P450 enzymes 
and not CYP3A4 or CYP3A5, in which case use of that drug may be approvable for use in the 
study. If in doubt, please discuss with the medical monitor.  
Systemic steroids (not inhalation or topical) in amounts of 20 mg or higher of prednisone or 
equivalent per day may be permitted under certain circumstances (e.g., as pre- and post-
medication for reactions to contrast agents; treatment of pre-existing disease-related 
autoimmune cytopenias and hemolysis; those treated for GVHD; those with an acute need for a 
non-disease related condition (e.g., additional steroid requirement for adrenal insufficiency or 
other acute condition where a short course [up to 14 days] may be required). In such 
circumstances of non-disease-related conditions, please discuss with the medical 
monitor/Sponsor regarding continuation of dosing of study drug during this period of increased 
steroid administration. Patients may not receive any other anticancer therapies for the current 
malignancy while on study prior to disease progression.  
Supportive care with blood products (prophylactic or therapeutic) and hematopoietic growth 
factors may be used in accordance with institutional standards, however the use of myeloid or 
erythroid growth factor during the DLT evaluation period should be discussed with the medical 
monitor and the study Sponsor. 
Cohorts using combination with acalabrutinib (B Cohorts)  
Concomitant strong CYP3A inhibitors, CYP3A inducers or proton pump inhibitors are prohibited. 
Dose modifications should be used according to the Prescribing Information and institutional 
standard of care for concomitant moderate CYP3A inhibitors. Treating physicians should avoid 
co-administration with proton pump inhibitors. If the patient is taking antacids, patients should 
be advised to separate dosing by at least 2 hours of taking antacids. If the patient is taking an 
H2 receptor antagonist, patients should be advised to take acalabrutinib 2 hours prior to the H2 
receptor antagonist. 

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Cohorts using combination with venetoclax (C Cohorts) 
Strong and moderate CYP3A inhibitors/inducers or strong P-gp inhibitors are prohibited. As 
MS-553 may be a P-gp substrate, patients must avoid taking venetoclax together with MS-553 
at the same time. A six-hour interval between MS-553 and venetoclax is advised.  
5.3. 
Withdrawal Criteria 
Patients will be advised that they are free to withdraw from the study at any time for any 
reason. A patient may be withdrawn from the study for any of the following reasons: 
• Patient voluntarily discontinues study participation (consent withdrawal); 
• The need to take medication which may interfere with study measurements; 
• Adverse events; 
• Disease progression; Exceptions may be allowed if continuing study drug beyond disease 
progression is needed and prior consent of the medical monitor is obtained;   
• Major violation or deviation of study protocol procedures; 
• Non-compliance of patient with protocol; 
• Patient is unable to comply with study procedures; 
• Withdrawal from the study is, in the Investigator’s judgment, in the patient’s best 
interest; 
• Patient is lost to follow-up; 
• Study termination by the Sponsor. 
 The reasons for withdrawal will be recorded on the CRF and included in the final report along 
with any adverse events and any necessary medical treatment. 
5.4. 
Discontinued Patients 
Notification of early patient discontinuation from the study and the reason for 
discontinuation will be made to the Sponsor (or designee) and will be clearly documented on 
the appropriate electronic CRF. If a patient is discontinued from the study early, all End of 
Treatment visit evaluations should be performed at the time of discontinuation, if possible. 
Patients who are withdrawn or discontinue early may be replaced at the discretion of the 
Sponsor. 

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6. 
STUDY PROCEDURES 
A calendar of study procedures to be conducted for each patient enrolled in the study is 
presented in a table in Appendix 1, as Appendix 1A (A Cohorts), 1B (B Cohorts), and 1C (C 
Cohorts), which are also described in the text that follows. 
The Investigator must document any deviation from protocol procedures and notify the 
Sponsor or designee. 
Safety and tolerability assessments will include regular monitoring of AEs, changes from 
baseline in laboratory variables, physical examinations, vital signs, and safety assessments. 
From the time that informed consent is obtained through the first administration of 
investigational drug product, the Investigator must record all SAEs, as well as any non- serious 
AEs related to protocol mandated procedures, on the adverse event electronic CRF. All other 
untoward medical occurrences observed during the Screening period, including exacerbation or 
changes in medical history, are to be captured on the medical history electronic CRF. 
6.1. 
Description of Study Procedures 
6.1.1. 
Patient Enrollment and Treatment Assignment 
Patient eligibility will be established at the conclusion of the Screening evaluations. During the 
dose escalation phase, assignment to dose level will be sequential and as described in 
Section 5.2 Treatment Plan. For the dose expansion phase, patients will be dosed at the MTD or 
initial RP2D identified during dose escalation. 
6.1.2. 
Screening Period 
Screening procedures shall be performed within 28 days prior to first dose of study drug unless 
otherwise stated.  Procedures may be conducted over multiple visits if necessary. 
6.1.3. 
Informed Consent 
Screening begins with the informed consent procedure. The Investigator is obliged to give the 
patient thorough information about the study and study related assessments, and the patient 
should be given ample time to consider his or her participation. All patients must sign and date 
the Institutional Review Board/Independent Ethics Committee (IRB/IEC) approved informed 
consent form before any study procedures are performed except where noted in the protocol in 
relation to standard of care procedures. Optional procedures require separate patient consent 
either within the main or a separate informed consent form, per IRB/IEC requirements. 
6.1.4. 
Disbursement of Study Medication 
6.1.4.1. 
Disbursement of MS-553 Study Medication 
Patients will be dispensed the appropriate amount of MS-553. One hundred eighty (180) or 120 
of the 100 mg MS-553 tablets in bottles at the 300, or 200 mg BID dose levels, respectively; one 
hundred twenty (120) 100 mg and sixty (60) 50 mg tablets at the 250 mg BID dose level, an 

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additional thirty (30) 25 mg tablets will be provided to DS1; one hundred twenty (120) 100 mg 
and sixty (60) 25 mg tablets at the 225 mg BID dose level (DS2); or sixty (60) 100 mg tablets at 
the 100 mg BID dose level. Patients will be dispensed the appropriate amount of 25 mg, 50 mg 
and/or 100 mg tablets of MS-553 based on their assigned dose level or dose adjustments. 
Additional tablets may be dispensed if necessary in the opinion of the investigator when the 
subsequent visit will be later than 28 days but within the 3-day window (see Appendix 1A (A 
Cohorts), 1B (B Cohorts), and 1C (C Cohorts). Refer to the Pharmacy Manual for more specific 
dispensation details. 
6.1.4.2. 
Disbursement of Acalabrutinib, Venetoclax, and Rituximab 
Patients will be dispensed the appropriate amount of acalabrutinib, venetoclax, and rituximab 
according to the prescribing information located in the package insert. The insurance provider 
and/or the patient will be responsible for the cost of acalabrutinib, venetoclax, and rituximab. 
6.1.5. 
Instruction to the Patient for Administration 
The Investigator must instruct the patient to take the study drug per protocol, the full 
Prescribing Information, and institutional standard of care for the approved combination drugs. 
All dosages prescribed and dispensed to the patient and any dose change or interruption must 
be recorded in the dosage administration record CRF as appropriate. Patients shall be instructed 
to record their doses in the provided study drug diary. Refer to the Pharmacy Manual for more 
detailed patient dosing instructions. 
6.1.6. 
Study Drug Accountability and Treatment Compliance 
At each clinic visit, patients will be asked to return any remaining study drug from the 
previous dosing cycle as well as all used and unused study drug containers.  The designated 
site staff will perform accountability as per each clinical site’s standard practice. 
For MS-553, the study site personnel are to supervise the patients taking study drug at the 
study site on days in which PK samples are taken. Patients are to keep a study drug diary of 
the date and time the study drug was taken for doses taken at home. The study drug diaries 
and used study drug packaging are to be returned to study site personnel at the next study 
visit. Study site personnel will review the study drug diary, examine the study drug packaging, 
and document their findings on the appropriate e-CRF. Designated site staff must thoroughly 
document and re-instruct the patient when significant noncompliance is noted. 
For the combination treatments, institutional standard procedures or patient diaries should 
be used for study drug accountability and treatment compliance recorded.  
All records must be readily available for inspection by the Sponsor or its representatives and 
the U.S. FDA or other regulatory authorities at any time. 
6.1.7. 
Dose Modifications and Dose Delay 
Investigators should follow the guidelines described herein for the modification of MS-553 
treatment. Any plan to deviate from these guidelines in view of patient safety must be 

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previously discussed with the study Sponsor and the medical monitor unless there is an 
urgent need for action. 
All dose modifications should be based on the worst preceding toxicity. If study treatment is 
being held due to toxicity, scheduled visits and all safety assessments in Cycle 1 should 
continue to occur, even without dispensation of the study drug. Additional scheduled visits 
and assessments including all PK samplings may be adjusted according to the actual dosing 
schedule upon the agreement between the investigator, the study medical monitor, and the 
Sponsor. 
Recommended modifications to dosing (not including dose-limiting toxicities in the first cycle) 
are described in Appendix 2A (MS-553 Recommended Dose Modifications and Delays for 
Toxicity). 
A maximum of three dose reduction steps but not lower than 100 mg BID as a single agent will 
be allowed, after which the patient will be discontinued from study treatment if there is a need 
for further dose reduction. In the combination regimens of cohort B and C, dose reductions to 
not lower than 50 mg BID is allowed.  
For patients who undergo dose interruptions (delays) due to toxicity, if the same toxicity returns 
after re-initiation of treatment, regardless of duration, the second re-initiation of study drug 
must resume at the next lower dose level. However, the dose of MS-553 that is restarted could 
be reduced if the investigator feels it prudent to re-challenge at a lower dose. Please refer to 
Appendix 2A for dose modification instructions.  
Recommended modifications to dose and schedule for the combination treatments are 
outlined in Appendix 2B and 2C, and according to institutional standard of care.  
If the patient requires a dose interruption of > 28 days from previous dose for either MS-553 or 
combination treatment, then the patient must be discontinued from study treatment. Patients 
who discontinue the treatment due to an adverse event or an abnormal laboratory value, even 
if not related to study drug, must be followed until resolution or stabilization of the event. 
All dose changes or interruptions must be recorded in the dosage administration record CRF. 
6.1.8. 
Permitted Concomitant Therapy 
In general, the use of any concomitant medication/therapy, including over-the-counter (OTC) 
medications, deemed necessary for the care of the patient is permitted during the study, with 
the exception of the prohibited medications noted in Section 5.2.5 and Section 6.1.10. 
Hormonal contraceptives are permitted in women of child-bearing potential (see Section 4.2). 
Hormonal contraceptives include any marketed contraceptive agent that includes an estrogen 
and/or a progestational agent. It is unknown whether MS-553 could affect the efficacy of 
hormonal contraceptives and therefore women of childbearing potential on study must agree 
to use at least two methods of contraception as outlined in the Exclusion Criteria. 
The patient must be told to notify the investigator and study personnel about any new 
medications, including OTC and herbal supplements that he or she begins after the 
commencement of the study drug. All medications (other than study drug) and significant non-

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drug therapies (including physical therapy and blood transfusions) administered during the 
study must be recorded.  
See Section 5.2.4 for additional information on concomitant and supportive therapy, 
instructions, in particular for the combination regimens.  
6.1.9. 
Permitted Concomitant Therapy to be Used with Caution 
Medication that are weak or moderate (as defined by the FDA; see the link in Appendix 3) 
inhibitors or inducers of CYP3A4/5 are not prohibited but should be administered with caution, 
as they may increase or decrease MS-553 exposure. (Moderate CYP3A inhibitors are prohibited 
in Cohort C.) Medications that are moderate inhibitors of CYP3A will require dose modifications 
as outlined in Section 5.2.5 for patients on the combination with acalabrutinib (Cohort B 
Series). Medications that are P-gp substrates must not be taken ≤ 6 hours prior to venetoclax 
(Cohort C Series) as outlined in Section 5.2.3.3.   
After Cycle 1, steroids at doses up to 20 mg prednisone or equivalent per day are allowed for 
symptomatic relief of lymphadenopathy and B symptoms, if needed.  
After completion of Cycle 1, higher doses of steroids may be used for therapeutic treatment of 
GVHD or autoimmune cytopenia or other intercurrent illness after discussion with the medical 
monitor and/or Sponsor.  
Systemic steroids (not inhalation or topical) in amounts of 20 mg or higher of prednisone or 
equivalent per day may be permitted under certain circumstances (e.g., as pre- and post-
medication for reactions to contrast agents; treatment of pre-existing disease-related 
autoimmune cytopenia and hemolysis; those treated for GVHD; those with an acute need for a 
non-disease related condition (e.g., additional steroid requirement for adrenal insufficiency or 
other acute condition where a short course [up to 14 days] may be required). In such 
circumstances of non-disease-related conditions, please discuss with the medical 
monitor/Sponsor regarding continuation of dosing of study drug during this period of increased 
steroid administration. 
6.1.10. 
Prohibited Concomitant Medications 
Strong inhibitors or inducers of CYP3A4/5 and sensitive CYP3A4/5 substrates and compounds 
with known QT prolongation risk as listed in their package inserts or belong to drugs with known 
risk of Torsades de Pointers (TdP) on crediblemeds.org should be avoided whenever possible 
and should be discontinued at least 48 hours prior to initiation of study drug. Refer to Appendix 
3 for examples of these medications. When substitution of another medication is not feasible, 
discussion with the medical monitor and Sponsor will be needed. For example, the metabolism of 
the drug in question may be more affected by other P450 enzymes and not CYP3A4 or CYP3A5, in 
such case use of that drug may be approvable for use in the study. If in doubt, please discuss with 
the medical monitor.   
Medications that are known to cause or have the potential to cause QTc prolongation as listed 
in their package inserts or belong to drugs with known risk of Torsades de Pointers (TdP) on 
crediblemeds.org should be avoided whenever possible unless prior approval of the Sponsor or 

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medical monitor is provided. Those with a history of increased risk for QT prolongation (e.g., 
congenital long QT-syndrome, low ventricular ejection fraction, impaired hepatic or renal 
function that could affect drug metabolism, uncorrected electrolyte disturbances, and history 
of drug-related prolonged QT interval) are addressed in Exclusion Criterion #13. When 
substitution of another medication has been approved by the Sponsor or medical monitor, 
patients taking medications known to cause QT/QTc prolongation as listed in as listed in their 
package inserts or belong to drugs with known risk of Torsades de Pointers (TdP) on 
crediblemeds.org should be monitored appropriately with serial ECGs. Refer to Section 5.2.5 
for the prohibited concomitant medications. 
6.1.11. 
Visit Schedule 
The schedule of visits including which study related procedures are to be performed and which 
samples are to be collected at each time point is described graphically in Appendix 1A (A 
Cohorts), 1B (B Cohorts), and 1C (C Cohorts). 
6.1.12. 
Visit Days and Numbering 
Mandatory visits include the Screening visit, C1D1, C1D8, and Day 1 (± 3 days) of subsequent 
cycles, and the End of Treatment (EOT) visit. Patients may be required to make visits for PK 
samplings. Other visits may occur as required for standard of care medical practice.  
If a patient comes early to begin a new cycle (for example, coming on Cycle 3 Day 28 would be 
equivalent to Cycle 4 Day -1), all study related procedures including, physical exam, clinical 
safety laboratory, CT scans if applicable, and study drug dispensing can occur, but Day 1 of the 
next cycle is not renumbered. 
If a patient comes late to begin a new cycle but has missed doses of study medication, the day 
that the study medication is resumed shall be designated Day 1 of the next cycle (i.e., the 
previous cycle is lengthened). 
If a patient’s temporary discontinuation of study medication overlaps Day 1 of a subsequent 
cycle, the day that the study medication is resumed shall be designated Day 1 of the next cycle 
(i.e., the previous cycle is lengthened). If a patient holds study drug mid-cycle but resumes 
before Day 1 of a subsequent cycle, the cycle days are not renumbered. 
Other alterations to the visit schedule, cycle numbering, or the ± 3 day window must be 
discussed with the medical monitor and recorded as a protocol deviation. 
6.2. 
Assessments of Safety, Efficacy, and Exploratory Endpoints 
6.2.1. 
Safety 
For Cohort A1, assessments for DLT and TEAE will occur during Cycle 1. For Cohort B1 and C1, 
assessments for DLT and TEAE will occur during defined DLT evaluation periods (see Sections 0 
and 3.3.4). TEAE will be assessed during the course of the trial. The primary endpoint will be the 
rate of DLT and TEAE requiring study drug discontinuation in the DLT evaluable period. 
Toxicities will be defined using NCI CTCAE v5.0 criteria in Appendix 4. 

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Safety assessments will consist of monitoring and recording all adverse events, including serious 
adverse events, the monitoring of hematology, blood chemistry, and the regular monitoring of 
vital signs, ECGs, and physical condition as shown in Appendix 1A (A Cohorts), 1B (B Cohorts), or 
1C (C Cohorts). For details on AE collection and reporting, refer to Section 7, Adverse Events and 
Toxicity Management.  If a patient has vision-related adverse event that is ≥ Grade 2 and 
deemed at least possibly related to the drug, the patient will be referred to an ophthalmologist 
for a typical 8-point exam including measurement of visual acuity, slit-lamp bio-microscopy, and 
dilated full-fundus examination of the macula, optic nerve and peripheral retina. 
6.2.2. 
Efficacy 
Response assessments will be made by the individual investigators. Response for CLL will be 
evaluated according to criteria published by 2008 IWCLL.  Because of the well-documented 
lymphocytosis not associated with disease progression that occurs with BCR signaling inhibitors 
such as BTK inhibitors, progressive lymphocytosis in the absence of other signs of disease 
progression (e.g., splenomegaly, enlarging lymph nodes, and disease-related constitutional 
symptoms) will not be considered disease progression. Isolated lymphocytosis in the context of 
what would otherwise be a PR is defined as a PR with lymphocytosis (noted as PR-L, PR+L, or 
PRwL in literature). Response criteria in the SLL patients and aggressive lymphoma patients will 
be based on the International Working Group consensus response evaluation criteria in lymphoma 
(RECIL 2017). 
Initial evaluation of the efficacy endpoints related to response will incorporate the data from 
the first 9 cycles of treatment. Any patient who receives at least one dose of study therapy is 
evaluable for response. Patients who have stable disease or a response by 9 cycles and 
continue treatment after 9 cycles will have response assessments including CT scans of the 
chest, abdomen, and pelvis (and neck if involved), preferably least every 12 weeks, or as 
clinically indicated until disease progression. Patients who do not have a response by 9 cycles 
but who have stable disease are eligible to stay on study treatment for up to a total of 24 
cycles, with continual re-evaluation for response at subsequent visits during this time period. 
For patients who continue to have stable disease after 24 cycles or have a response after 9 
cycles therapy, drug therapy can continue until disease progression. 
Secondary efficacy endpoints in all cohorts include overall response rate (ORR), duration of 
response (DoR), disease control rate (DCR), PFS, and OS. For patients achieving a response, the 
response duration will be measured from the time at which criteria for CR, PR, or PR with 
lymphocytosis is first assessed until the date at which recurrent or progressive disease or death 
due to disease is documented. Stable disease, a component of DCR, is defined as at least 6 
months from the start of MS-553 monotherapy until to first documentation of progressive 
disease (PD) in relapsed and refractory CLL/SLL patients. Progression free survival will be 
measured as the time from the first dose of the study drug until disease progression or death 
from any cause. Overall survival will be measured as the time from the first dose of the study 
drug until death due to any cause. For the secondary efficacy endpoints of response duration, 
PFS, and OS, patients who have not progressed or died at the time of last known status will be 
censored at that time; for response duration and PFS, patients may also be censored at the time 

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of subsequent treatment if a prior progression is not documented. See Section 8, Statistics, for 
more complete details on censoring. 
6.2.3. 
Pharmacokinetics and Exploratory 
Flow cytometry for diagnosis will be performed at screening on both bone marrow (when 
available) and peripheral blood samples (all patients). Flow cytometry will be performed on 
blood samples at other visits at the investigator’s discretion.  
 
 
 
 
 
Pharmacokinetics of MS-553 and/or its metabolites will be studied. Please see table below for 
timing of PK assessment. The exact time of each PK draw must be accurately recorded in the 
source document and on respective eCRFs. 
Table 7:  
Schedule of PK Assessments 
 
C1D1 
C1D8 
C2D1 
C2D8 
C3D8 
C4D1 
C4D8 
C5D1 
Cohort A1 
X 
X 
 
 
 
 
 
 
Cohort A2 
 
X 
 
X1 
X1 
X 
 
 
Cohort A2 DS1 
 
 
X 
 
 
 
 
 
Cohort A2 DS2 
 
 
X 
 
 
 
 
 
Cohort A3 
 
 
Y 
 
 
 
 
 
Cohort B1 
 
200 mg BID 
 
X 
Y 
 
 
 
 
 
>200 mg BID 
 
X 
 
X1 
X1 
Y 
 
 
 
Cohort B2 & 
B3 
 
 
200 mg BID 
 
 
Y 
 
 
 
 
 
>200 mg BID 
 
 
 
 
 
Y 
 
 
Cohort C 
 
 
C1 
 
 
 
 
Z 
X 
 
 X 
C2 
 
 
 
 
 
 
 
Y 
DS1: Dose Schedule 1 
DS2: Dose Schedule 2 
X: PK sampling timepoints: MS-553 pre-dose, and 1 hr, 2 hr, 3 hr, and 4 hr (± 5 minutes) post-dose, and 6 hr (± 10 
minutes) post-dose 
Y: PK Sampling timepoint: MS-553 pre-dose 
Z: PK sampling time points: 1 hr, 2 hr, 3 hr, and 4 hr (± 5 minutes), and 6 hr (± 10 minutes) post-venetoclax dose 
1: Patients need to return for PK blood draws. 

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6.3. 
  Correlative Science 
Correlative studies are critical to understanding toxicity and efficacy of novel targeted agents. 
Tightly coupling correlative studies with clinical trials can help understand best next lines of 
therapies for patients as well as predictors of response and mechanisms of resistance in a much 
more rapid manner. The samples collected and procedures performed for correlative laboratory 
science are an integral part of this study. Complete details of correlative science sample 
collection, analyses and end points are provided in the Lab Manual. 
6.3.1. 
Responsibilities 
MingSight will coordinate the collection and analysis of all correlative science and exploratory 
studies. 
6.3.2. 
Correlative Sciences Sample Collection Overview 
There are three types of samples being collected for the correlative sciences studies to be 
performed. They are: 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
  
 
 
 
3) Pharmacodynamic samples (PKC-β Biomarkers) for the measurement of PKC-β signalling 
in cells isolated from whole blood. 
 
 
 
 

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ould be done every 3 cycles for three assessments after patient 
 
6.3.3. 
Correlative Science Studies Description 
Several key study time points for correlative sample collection are planned (see Section 
6.3.2). For responding patients, some samples may be collected during follow up therapy. 
 will be ideally collected at Cycle 1 Day 1 using the 
 
 
  
For Cohorts A1, A2, and B1 
 will 
be collected immediately prior to dosing on Cycle 1 Day 1 and Cycle 1 Day 8 pre-dose and at 3 
hr post-dose.  For Cohort C1, PKC-β biomarker samples will be collected prior to dosing on 
Cycle 4 Day 1 and pre-dose and at 3 hr post-dose on Cycle 4 Day 8.  
 
 
 
 
 
 
 
For all cohorts, research samples will be taken f
 for CLL 
patients and Richter’s from CLL. For all patients in Cohort A, additional research samples will be 
taken for 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

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7. 
ADVERSE EVENTS AND TOXICITY MANAGEMENT 
7.1. 
Dose Limiting Toxicities 
Determination of DLTs occurs only during the DLT periods defined in Section 3 for Cohorts A1, B1, 
and C1. See Study Procedures for DLT definitions. 
7.2. 
Modification or Holding of Dose for Toxicities 
Doses of MS-553 can be decreased or held for toxicities for events deemed possibly, probably, 
or definitely related to MS-553. Refer to Section 6.1.7, Dose modifications and dose delay and 
Appendix 2A: MS-553 Recommended Dose Modifications and Delays for Toxicity, Appendix 2B 
and Appendix 2C for more information and specific guidance. The dose of MS-553 that is 
restarted could be reduced if the investigator feels it prudent to re-challenge at a lower dose, 
and if the same toxicity does not recur approximately after four weeks, the dose may be 
increased back to the patient’s prior dose. Discussion with the medical monitor/Sponsor are 
needed for such dose modifications. 
7.3. 
Adverse Events 
Any untoward medical occurrence in a patient or clinical investigation subject administered a 
pharmaceutical product and which does not necessarily have to have a causal relationship with 
this treatment. An adverse event (AE) can therefore be any unfavorable and unintended sign 
(including an abnormal laboratory finding, for example), symptom, or disease temporally 
associated with the use of a medicinal product, whether or not considered related to the 
medicinal product (FDA Guidance for Industry 1995). In addition, AEs may also represent pre- or 
post-treatment complications that occur as a result of protocol-specified procedures. For the 
purposes of this clinical study, AEs shall include events which are either new or represent 
detectable worsening of pre-existing conditions that occur as a consequence of participation in 
the clinical study. The following are expressly not considered AEs: 
Pre-existing conditions  
• Pre-existing conditions (documented in the medical history CRF) unless the 
severity, frequency, or character is exacerbated during the study period 
• Hospitalization except for elective hospitalizations that had been planned for 
preexisting conditions that have not worsened. However, if the pre-planned 
hospitalization is lengthened as a result of participation in the study or if during 
the pre-planned hospitalization an adverse event, meeting SAE criteria, occurs 
then the event(s) are considered SAEs. 
Abnormal laboratory results requiring treatment or that are assessed as related to the study 
drug are captured as AEs.  If the results of the laboratory abnormality do not require treatment 
and is assessed as not related to the study drug, they will not be considered AEs and will be 
assessed as part of safety with the laboratory assessments.  

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7.4. 
Serious Adverse Event 
The ICH topic E2 on Clinical Safety Data Management, Definitions, and Standards for Expedited 
Reporting defines a serious adverse event (or experience; SAE) or reaction as any untoward 
medical occurrence that at any dose: 
• Results in death 
• Is life-threatening. The term "life-threatening" in the definition of "serious" refers 
to an event in which the patient was at risk of death at the time of the event. It 
does not refer to an event which hypothetically might have caused death if it were 
more severe. 
• Requires inpatient hospitalization or prolongation of existing hospitalization of at least 
24 hours. 
• Results in persistent or significant disability/incapacity. The term disability means a 
substantial disruption of a person’s ability to conduct normal life functions. This 
definition is not intended to include experiences of relatively minor medical 
significance such as uncomplicated headache, nausea, vomiting, diarrhea, influenza, 
and accidental trauma (e.g., sprained ankle) which may interfere or prevent everyday 
life functions but do not constitute a substantial disruption. 
• Is a congenital anomaly/birth defect (i.e., an adverse finding in a child or fetus or a 
subject exposed to the study medication prior to conception or during pregnancy). 
• An important medical event 
NOTE: Important medical events that may not result in death, be life- threatening, or 
require hospitalization may be considered an SAE when, based upon appropriate 
medical judgment, they may jeopardize the subject and may require medical or 
surgical intervention to prevent one of the outcomes listed above. Examples of such 
medical events include allergic bronchospasm requiring intensive treatment in an 
emergency room or at home, blood dyscrasias or convulsions that do not result in 
inpatient hospitalizations, or the development of drug dependency. (FDA Guidance for 
Industry 1995). 
Given the unique and important perspectives of both the investigator and the Sponsor, 
if either considers that the event is serious, the event will be considered an SAE. 
Serious adverse events should be reported within 24 hours of discovery. Death is an 
outcome of an AE/SAE rather than an SAE itself and should be reported as the 
antecedent proximate cause.   
Disease progression (CLL/SLL/Lymphoma) resulting in death will not be captured as an SAE 
but will be captured on the specific CRF. 
Elective hospitalizations that had been planned for starting a new treatment or for preexisting 
conditions that have not worsened are not considered SAEs. 

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7.5. 
Definition and Grading of Toxicities 
Toxicities will be defined using NCI CTCAE v5.0 criteria. All toxicities should be graded 1-5 
according to the standardized scale laid out therein. 
7.6. 
Causality of Adverse Events 
The investigator should use his or her best judgement to identify one of the following categories 
for causality / relatedness of the AE to the study medication. 
1. Unrelated – The Adverse Event is clearly not related to the investigational agent(s) 
2. Unlikely –  The Adverse Event is doubtfully related to the investigational agent(s) 
3. Possible – The Adverse Event may be related to the investigational agent(s) 
4. Probable – The Adverse Event is likely related to the investigational agent(s) 
5. Definite – The Adverse Event is clearly related to the investigational agent(s). 
7.7. 
Documentation of Adverse Events and Serious Adverse Events 
7.7.1. 
Reporting Period 
Adverse events (including SAEs) will be documented on the AE eCRF and monitored 
continuously throughout the study from the time of first dose of study drug until 30 days (+ 7 
days) after the patient’s last dose of study drug or until the event has resolved, stabilized, or 
an outcome has been reached, whichever comes first regardless of the timing of the End of 
Treatment visit. If the End of Treatment visit occurs prior to 30 days (± 7 days) after the date 
of last dose, then a toxicity assessment should be performed either in person or via 
telephone to capture AEs through the AE reporting period. In addition, SAEs occurring 
between the time of signing of informed consent form and the first dose of the study drug 
must be reported. SAEs related to the study drug and occurring after the end of the AE 
reporting period must be reported. 
For patients who discontinue study drug due to a study-related AE, the reporting time period 
may be extended. These patients must be followed at least once a week for four weeks, and 
subsequently at four-week intervals until resolution or stabilization of the adverse event or 
laboratory abnormality, whichever comes first. Additionally, the frequency of AE monitoring 
will increase for patients who experience a DLT at the discretion of the investigator. 
If the investigator considers it necessary to report an AE in a study patient occurring after the 
end of study, he or she should contact the Sponsor to determine how the AE should be 
documented and reported. 
7.7.2. 
Assessment of Adverse Events 
Investigators will assess the occurrence of AEs and SAEs at all patient evaluation time points 
during the study. All AEs and SAEs whether volunteered by the patient, discovered by study 

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personnel, or detected through physical or laboratory examination, or by any other means will 
be recorded in the patient’s medical record and on the AE CRF. 
Each assessment of AEs or SAEs will be described with the prescribed terminology including 
grade, with start and end (if applicable) dates, regulatory seriousness criteria (if applicable), 
suspected relationship to study drug, and any action(s) taken in response. 
7.8. 
Procedures for Reporting Serious Adverse Events 
All serious adverse events occurring during study participation must be reported to the Sponsor 
(or designee) and to the governing IRB/IEC as required by the IRB/IEC, local regulations, and the 
governing health authorities. 
7.8.1. 
Completion and Transmission of SAE Report 
Once an Investigator becomes aware that an SAE has occurred in a study patient, she/he will 
report the information to the Sponsor (or designee) within 24 hours. The eSAE form in the EDC 
will always be completed as thoroughly as possible with all available details of the event and 
submitted to the Sponsor (or designee) within the designated time frames. If the Investigator 
does not have all information regarding an SAE, he/she will not wait to receive additional 
information before notifying the Sponsor (or designee) of the event and completing the form. 
The form will be updated when additional information is received. Pertinent follow-up 
information is also reported within 24 hours of the site becoming aware of the updates. 
The Investigator will provide an assessment of causality at the time of the initial report as 
described above.  If the EDC system is not operational (e.g., system malfunction) at the time 
of the SAE, a paper SAE report may be completed and faxed to the Safety team.  Paper SAE 
templates will be provided to the Investigator prior to study start. 
 
 
The Sponsor will provide a list of project contacts for SAE receipt. Any event that in the opinion 
of the Investigator may be of immediate or potential concern for the patient’s health or well-
being will be reported to the Sponsor (or designee).  
7.8.2. 
Regulatory Reporting Requirements for Serious Adverse Events 
The Investigator must promptly report all SAEs to the Sponsor in accordance with the 
procedures describe above. Prompt notification of SAEs by the Investigator to the 
appropriate project contact for SAE receipt is essential so that legal obligations and ethical 
responsibilities towards the safety of other patients are met. The Investigator will be 
responsible for reporting SAEs to the IRB/IEC per local regulatory requirements. 
The Sponsor (or designee) is responsible for reporting SAEs to the relevant regulatory 
authorities in accordance with local regulations. That is, SAEs which are “Unexpected” and 
classified as “Suspected Adverse Reactions” or “Adverse Reactions” will be reported. 

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The Sponsor (or designee) is responsible for submitting reports to the FDA of unexpected SAEs 
considered to be possibly related to the administration of study drug, according to 21 Code of 
Federal Regulations 312.32. All Investigators participating in ongoing clinical studies with the 
study drug will receive copies of these reports for prompt submission to their IRB/IEC. 
Investigators are to provide a copy of the written IRB notification for each report to the 
Sponsor or its designee. 
An adverse event is considered “Unexpected” if it is not listed in the investigator brochure or is 
not listed at the specificity or severity that is observed in the present study. 
7.9. 
Pregnancy 
Pregnancy is an exclusion criterion. All patients should be instructed to immediately notify the 
investigator and study staff of pregnancy that occurs during the study period. Any female 
patient who becomes pregnant should immediately discontinue the study medication. Although 
pregnancy itself is not an AE, if a study patient or the partner of a study patient should become 
pregnant during the course of the study this should be reported on a pregnancy report form and 
the pregnancy should be followed to conclusion with the outcome(s) including any untoward 
events in mother or child reported to the study Sponsor. 
7.10. 
Follow Up of Adverse Events and Serious Adverse Events 
All AEs and SAEs occurring during the study are to receive appropriate medical treatment and 
be followed up in accordance with good medical practice until resolved or judged no longer 
clinically significant, or if a chronic condition, until fully characterized. Relevant follow-up 
information is to be provided to the Sponsor or its designee. 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

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7.12. 
Management of Overdose and Toxicity 
There are no specific treatments for overdose. In the case of accidental or intentional overdose 
of MS-553, standard supportive measures for overdose should be employed. 
7.13. 
Study-wide Stopping Rule for Excessive Toxicity 
To ensure patient safety, the sponsor will implement a set of study-wide stopping rules as 
described below.  
• The sponsor shall evaluate adverse events that occur throughout the duration of 
therapy beyond the DLT window. 
• The sponsor shall evaluate all adverse events at least quarterly throughout the study. 
• 
 
 
 
 
 
 
 
• The study stopping rule shall be triggered if the rate of major hemorrhage events 
(serious or any ≥ Grade 3 bleeding or any central nervous system bleeding) is ≥ 4.2%, 
the rate of major hemorrhage events listed in the product label of ibrutinib. 

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• The study stopping rule shall be triggered if an SAE considered to be at least possibly 
related to the study drug results in death. 
• The sponsor shall also report to the Safety Review Committee any additional serious or  
≥ Grade 3 adverse events that are above reference rates in CLL patients.   
Once a stopping rule is triggered, the study shall be paused for enrollment and a 
comprehensive safety review shall be conducted by the Safety Review Committee. After this 
review, the SRC will determine whether to recommend resuming treatment with appropriate 
safety measures. 
    

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8. 
STATISTICAL CONSIDERATIONS AND DATA ANALYSIS 
8.1. 
General Considerations 
Summary statistics will be provided for all endpoints. Statistics will be reported according to 
intention-to-treat (ITT), per-protocol, and per-dose-level. The ITT summary statistics will 
include any patient who received at least one dose of study drug. As a phase I study, the ITT 
population also serves as the safety population. 
Patient characteristics will be described and summarized for each dose level and in total.  
8.2. 
Sample Size Calculations 
The projected sample size was estimated on the basis of a standard 3+3 design plus a 
mandatory expansion cohort, plus two optional non-comparative expansion cohorts. This study 
is not powered to detect differences in tolerability/safety or efficacy between cohorts. 
8.3. 
Dose Escalation Criteria 
Because disease-relevant pharmacodynamics biomarkers are not yet established, and some 
limited toxicity and pharmacokinetic data in healthy subjects exists, dose escalation will be 
guided by toxicity. 
8.4. 
Determination of Sample Size 
There is no formal hypothesis to be tested in this study; therefore, no predefined sample size 
calculation has been made for the efficacy or exploratory endpoints. 
8.5. 
Analysis Populations 
8.5.1. 
Intention to Treat 
The intention to treat (ITT) population shall be defined as all patients who sign the informed 
consent form and received at least one dose of study drug. This population will be used primarily 
for the analysis of safety data. Summary statistics for efficacy will also be performed on the ITT 
population. 
8.5.2. 
Per Protocol Population 
The per-protocol population shall be defined as all patients who received at least one dose of 
study drug and who within the limits of the protocol completed an entire prescribed course of 
treatment, terminating at disease progression or after the maximum number of cycles. Efficacy 
summary statistics will also be reported for this population. 
8.5.3. 
Pharmacokinetic Population 
The PK population shall be defined as all patients who received at least one dose of study drug 
and had at least one post-treatment PK sample properly collected and analyzed. 
Pharmacokinetic summary statistics will be reported in this population.  

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8.5.4. 
Pharmacodynamic Population 
The population shall be defined as all patients who received at least one dose of study drug and 
had at least one post-treatment PD sample properly collected and analyzed. Pharmacodynamic 
biomarker data summary statistics will be reported in this population. 
8.6. 
Demographic Data 
Demographic and baseline measurements will be summarized using standard descriptive 
methods on the ITT population. 
8.7. 
Summary Statistics 
Tables of summary statistics over the foregoing analysis populations shall include the 
following for continuous variables: N (number in analysis set); n (number with data), mean, 
standard deviation, median, minimum, and maximum.  95% confidence intervals will also be 
presented for select continuous endpoints. 
Tables of summary statistics over the foregoing analysis populations shall include the 
following for categorical variables: N, n, percentage, and 95% confidence intervals will also 
be presented for select categorical endpoints. 
Analyses will be based on the observed data, unless specific methods for handling missing data 
are specified herein. 
8.8. 
Secondary Analyses: Efficacy 
ORR will be calculated along with its 95% CI based on exact method. In the analysis of ORR, 
patients who do not have sufficient on-study tumor assessment to characterize response will 
be excluded from the denominator. 
 
 
 
 
 
 
 
 
 
 
 
 
 
8.9. 
Exploratory Analyses 
Summary statistics and other assay-appropriate measures will be provided for all exploratory 
analyses (
 

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8.10. 
Protocol Deviations and Violations 
The Investigator is responsible for ensuring that the study is conducted in accordance with the 
protocol. No modifications to the protocol, other than those that are deemed necessary to 
protect the safety, rights, or welfare of patients by the Investigator are to be made without 
prior, written approval by the Sponsor. The nature and reasons for the protocol deviation will 
be recorded where appropriate and indicated. The Sponsor must be notified of all protocol 
deviations. Significant protocol deviations (e.g., inclusion/exclusion criteria) will be reported to 
the Sponsor and to the IRB/IEC in accordance with its reporting policy. 
8.11. 
Data Recording 
An electronic CRF (eCRF) will be used. In some cases, direct entry of data into the eCRF will 
result in the eCRF being considered the source document. The eCRF will be an FDA 21 CFR part 
11 compliant database. 
8.12. 
Data Quality Control 
Data recorded in patient eCRFs will be subjected to a quality control review. Persons 
responsible for any data entry, calculations and/or transcriptions will each sign and date for the 
work performed.  

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9. 
REGULATORY AND ETHICAL CONSIDERATIONS 
9.1. 
Summary Statistics Regulatory and Ethical Compliance: Good Clinical 
Practice 
This study must be implemented and carried out in accordance with the protocol, the ICH 
Harmonized Tripartite Guidelines for Good Clinical Practice, with applicable local regulations 
(US Code of Federal Regulations Title 21 including parts 50 and 56 concerning informed 
consent and IRB regulations; European Directive 2001/20/EC and 91/507/EEC), and in 
accordance with the ethical principles laid down in the Declaration of Helsinki concerning 
medical research in humans (Recommendations Guiding Physicians in Biomedical Research 
Involving Human Subjects, Helsinki 1964, amended Tokyo 1975, Venice 1983, Hong Kong 
1989, Somerset West 1996). 
The Investigator agrees, when signing the protocol, to adhere to the instructions and 
procedures described in it and thereby to adhere to the principles of Good Clinical Practice 
that it conforms to. 
Study investigators shall have current training in Good Clinical Practice. 
9.2. 
 Institutional Review Board/Independent Ethics Committee 
Before implementing this study, the protocol, the proposed informed consent form and other 
information to patients, must be reviewed by a properly constituted IRB/IEC. A signed and 
dated statement that the protocol and informed consent have been approved by the IRB/IEC 
must be kept in study binders on site and given to the Sponsor before study initiation. The 
name and occupation of the chairman and the members of the IRB/IEC must be supplied to 
the study Sponsor. Any amendments to the protocol, other than administrative ones, must be 
approved by this committee. Additionally, amendments to the protocol must be reviewed and 
approved by the study Sponsor prior to implementation. 
The Investigator will not implement any changes to the protocol without prior written 
approval of an appropriate amendment by the IRB, except when necessary to eliminate 
immediate hazards to the patients or when the change(s) involves only logistical or 
administrative aspects of the study (e.g., change of monitor or telephone numbers). 
The Investigator will promptly report to the IRB (per local requirements) any of the following:  
• Deviations from the protocol. 
• Changes to the protocol to eliminate immediate hazards to patients that were 
implemented without prior approval. 
• All SAEs that are considered at least possibly related to study drug and are unexpected 
(i.e., the nature or severity is not consistent with information provided in the current IB). 
• New information that may adversely affect the safety of the patients or the conduct of 
the study. 
• Termination or completion of the study. 

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The Investigator will comply with the IRB requirements for periodic (at least annual) review of 
the study until such time as all patients have concluded participation at the Investigator’s site, 
and with all other requirements established by the IRB and local regulations. 
9.3. 
Informed Consent 
The Investigator must explain to each patient (or legally authorized representative) the nature 
of the study, its purpose, the procedures involved, the expected duration, the potential risks and 
benefits involved and any discomfort it may entail. Each patient must be informed that 
participation in the study is voluntary and that he/she may withdraw from the study at any time 
and that withdrawal of consent will not affect his/her subsequent medical treatment or 
relationship with the treating physician. 
This informed consent should be given by means of a standard written statement, written in 
non-technical language. The patient should read and consider the statement before signing 
and dating it and should be given a copy of the signed document. If the patient cannot read or 
sign the documents, oral presentation may be made or signature given by the patient’s legally 
appointed representative, if witnessed by a person not involved in the study, mentioning that 
the patient could not read or sign the documents. No patient can enter the study before 
his/her informed consent has been obtained.  
The informed consent form is considered to be part of the protocol and must be submitted 
by the Investigator with it for IRB/IEC approval. 
The informed consent document should timely be amended as necessary to reflect changes in 
the protocol over time. 
9.4. 
Amendments to the Protocol 
Any change or addition to this protocol requires a written protocol amendment that must be 
approved by the investigator and the study Sponsor before implementation. Amendments 
significantly affecting the safety of patients, the scope of the investigation or the scientific 
quality of the study, require additional approval by the IRB/IEC. A copy of the written approval 
of the IRB/IEC, must be sent to the study Sponsor. 
Amendments affecting only administrative aspects of the study do not require formal protocol 
amendments or IRB/IEC approval but the IRB/IEC of each center must be kept informed of such 
administrative changes  
9.5. 
Files and Records Retention 
The Investigator and other appropriate study staff are responsible for maintaining all 
documentation relevant to the study. Mandatory documentation includes copies of study 
protocols and amendments, each FDA Form 1572, IRB/IEC approval letters, signed ICFs, drug 
accountability records, SAE forms transmitted to the Sponsor, patient files (source 
documentation) that substantiate entries in CRFs, all relevant correspondence and other 
documents pertaining to the conduct of the study. 

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An Investigator shall retain records for a period of at least 2 years after the date the last 
marketing application is approved for the drug for the indication for which it is being 
investigated; or, if no application is to be filed or if the application is not approved for such 
indication, until 2 years after the investigation is discontinued and regulatory authorities have 
been notified. Local regulations may require a longer period for record retention; said local 
regulations will supersede this protocol. The Investigator must notify the Sponsor and obtain 
written approval from the Sponsor before destroying any clinical study records at any time. 
The Sponsor will in turn inform the Investigator of the date that study records may be 
destroyed or returned.  
9.6. 
Publication of Results 
Any formal presentation or publication of data from this trial may be published after review 
and comment by the Sponsor and prior to any outside submission. The Sponsor must receive 
copies of any intended communication in advance of publication (at least fifteen working days 
for presentational materials and abstracts and twenty working days for manuscripts). These 
requirements acknowledge the Sponsor’s responsibility to provide peer input regarding the 
scientific content and conclusions of such publications or presentations. Principal 
Investigator/Institution shall have the final authority to determine the scope and content of 
its publications, provided such authority shall be exercised with reasonable regard for the 
interests of the Sponsor and, in accord with the trial contract and shall not permit disclosure 
of Sponsor’s confidential or proprietary information. 
Authorship, in general, will follow the recommendations of the International Committee of 
Medical Journal Editors (International Committee of Medical Journal Editors 2014). 
The stipulation on “publication of results” will be finalized in a contract regarding this clinical 
study between the Institution and the Sponsor. In case of any conflicts between this protocol 
and the contract regarding “publication of results”, the contract will prevail. 
9.7. 
Disclosure and Confidentiality 
The Investigator agrees to keep all information provided by the Sponsor in strict confidence 
and to request similar confidentiality from his/her staff and the IRB/IEC. Study documents 
provided by the Sponsor (Investigator’s Brochure and other material) will be stored 
appropriately to ensure their confidentiality. The information provided by the Sponsor to the 
Investigator may not be disclosed to others without direct written authorization from the 
Sponsor, except to the extent necessary to obtain informed consent from patients who wish 
to participate in the trial. 
The stipulation on “disclosure and confidentiality” will be finalized in a contract regarding this 
clinical study between the Institution and the Sponsor. In case of any conflicts between this 
protocol and the contract regarding “disclosure and confidentiality”, the contract will prevail.  

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9.8. 
DEFINITION OF END OF TRIAL  
9.8.1. 
End of Trial Participation in a Member State of the European Union 
End of trial participation in a Member State of the European Union is defined as the time at 
which it is deemed that a sufficient number of subjects have been recruited and completed the 
study as stated in the regulatory application (i.e., clinical trial application [CTA]) and ethics 
application in the Member State. Poor recruitment (recruiting less than the anticipated number 
in the CTA) by a Member State is not a reason for premature termination but is considered a 
normal conclusion to the study in that Member State.  
9.8.2. 
End of Trial in All Other Participating Countries  
End of trial in all other participating countries is defined as last subject last visit (LSLV).  
 

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Page 95 of 120  
 
 
10. 
REFERENCES 
1. Siegel Siegel R, Naishadham D, Jemal A. Cancer statistics, 2013. CA. Cancer J. Clin. 
2013;63(1):11–30. 
2. Calissano C, Damle RN, Hayes G, et al. In vivo intraclonal and interclonal kinetic 
heterogeneity in B-cell chronic lymphocytic leukemia. Blood. 2009;114(23):4832– 
4842. 
3. Messmer BT, Messmer D, Allen SL, et al. In vivo measurements document the 
dynamic cellular kinetics of chronic lymphocytic leukemia B cells. J. Clin. Invest. 
2005;115(3):755–764. 
4. Woyach JA, Johnson AJ, Byrd JC. The B-cell receptor signaling pathway as a 
therapeutic target in CLL. Blood. 2012;120(6):1175–1184. 
5. Byrd JC, Furman RR, Coutre SE, et al. Targeting BTK with Ibrutinib in Relapsed 
Chronic Lymphocytic Leukemia. N. Engl. J. Med. 2013;369(1):32–42. 
6. Burger JA, Tedeschi A, Barr PM, et al. Ibrutinib as Initial Therapy for Patients with 
Chronic Lymphocytic Leukemia. N. Engl. J. Med. 2015;373(25):2425–2437. 
7. Chang BY, Furman RR, Zapatka M, et al. Use of tumor genomic profiling to reveal 
mechanisms of resistance to the BTK inhibitor ibrutinib in chronic lymphocytic 
leukemia (CLL). J. Clin. Oncol. 2013;31:suppl; abstr 7014. 
8. Burger JA, Chiorazzi N. B cell receptor signaling in chronic lymphocytic leukemia. 
Trends Immunol. 2013 Dec;34(12):592-60. 
9. Mellor H, Parker PJ. The extended protein kinase C superfamily. Biochem. J. 
1998;332 ( Pt 2):281–292. 
10. Abrams ST, Lakum T, Lin K, et al. B-cell receptor signaling in chronic lymphocytic 
leukemia cells is regulated by overexpressed active protein kinase CβII. Blood. 
2007;109(3):1193–1201. 
11. Byrd JC, Shinn C, Willis CR, et al. UCN-01 induces cytotoxicity toward human CLL cells 
through a p53-independent mechanism. Exp. Hematol. 2001;29(6):703–708. 
12. Alkan S, Huang Q, Ergin M, et al. Survival role of protein kinase C (PKC) in chronic 
lymphocytic leukemia and determination of isoform expression pattern and genes 
altered by PKC inhibition. Am. J. Hematol. 2005;79(2):97–106. 
13. Holler C, Piñón JD, Denk U, et al. PKCβ is essential for the development of chronic 
lymphocytic leukemia in the TCL1 transgenic mouse model: validation of PKCβ as a 
therapeutic target in chronic lymphocytic leukemia. Blood. 2009;113(12):2791– 
2794. 
14. Ringshausen I, Schneller F, Bogner C, et al. Constitutively activated 
phosphatidylinositol-3 kinase (PI-3K) is involved in the defect of apoptosis in B-CLL: 
association with protein kinase Cδ. Blood. 2002;100(10):3741–3748. 

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15. Lutzny G, Kocher T, Schmidt-Supprian M, et al. Protein Kinase C-β-Dependent 
Activation of NF-κB in Stromal Cells Is Indispensable for the Survival of Chronic 
Lymphocytic Leukemia B Cells In Vivo. Cancer Cell. 2013;23(1):77–92. 
16. Hallek M, Cheson BD, Catovsky D, et al. Guidelines for the diagnosis and treatment of 
chronic lymphocytic leukemia: a report from the International Workshop on Chronic 
Lymphocytic Leukemia updating the National Cancer Institute–Working Group 1996 
guidelines. Blood. 2008;111(12):5446 –5456. 
17. Cheson BD, Byrd JC, Rai KR, et al. Novel Targeted Agents and the Need to Refine 
Clinical End Points in Chronic Lymphocytic Leukemia. J. Clin. Oncol. 
2012;30(23):2820–2822. 
18. Oken MM, Creech RH, Tormey DC, et al. Toxicity and response criteria of the Eastern 
Cooperative Oncology Group. Am. J. Clin. Oncol. 1982;5(6):649–655. 
19. Flockhart D. Drug Interactions: Cytochrome P450 Drug Interaction Table. Indiana 
University School of Medicine. Version 5.0 released on January 12, 2009. 
20. Tang CPS, McMullen J, Tam C1;Cardiac side effects of bruton tyrosine kinase (BTK) 
inhibitors.    Leuk Lymphoma. 2018 Jul;59(7):1554-1564. doi: 
10.1080/10428194.2017.1375110. Epub 2017 Sep 13 
 

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11. 
APPENDICES 

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APPENDIX 1A: STUDY CALENDAR A COHORTS (MS-553 MONOTHERAPY) 
Visit Evaluation Schedule 
Study Cycle (28 days) 
0 
1 
2 
3 
4+ 
EOTa 
S/RFUb 
Cycle dayi 
 
Study Procedure/Assessment 
Screeningh 
1 
8 
 1 (± 3 days) 
1 (±3 days) 
1 (±3 days) 
 
±7 days 
Q12 wks ±7d 
for response or Q24 
wks ±7d for survival 
 
 
 
 
 
 
 
 
 
 
Study Drug Dispensation 
Continuin
ollow-
ntil disease progression, toxicity, or other withdrawal
 
MS-553 PO BID
c
 
 
X 
 
X 
X 
X 
 
 
Procedures  
 
Informed Consent 
X 
 
 
 
 
 
 
 
Confirmation of inclusion/exclusion criteria 
X 
 
 
 
 
 
 
 
Demographics 
X 
 
 
 
 
 
 
 
Medical History and Physical Exam 
X 
X 
X 
X 
X 
X 
X 
X 
Concomitant medication survey 
X 
X 
X 
X 
X 
X 
X 
 
Adverse Events Assessmentp 
 
X 
X 
X 
X 
X 
X 
 
Vital signs; heightk/weight 
X 
X 
X 
X 
X 
X 
X 
X 
WHO/ECOG Performance Status 
X 
X 
X 
X 
X 
X 
X 
X 
ElectrocardiogramJ 
X 
X 
X 
X 
 
 
 
 
 Laboratory Studies  
 
 
 
Complete blood count with differential 
X 
X 
X 
X 
X 
X 
X 
X 
Serum chemistrye
 
X 
X 
X 
X 
X 
X 
X 
 
Urinalysis 
X 
 
 
 
 
 
 
 

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Study Cycle (28 days) 
0 
1 
2 
3 
4+ 
EOTa 
S/RFUb 
Cycle dayi 
 
Study Procedure/Assessment 
Screeningh 
1 
8 
 1 (± 3 days) 
1 (±3 days) 
1 (±3 days) 
 
±7 days 
Q12 wks ±7d 
for response or Q24 
wks ±7d for survival 
Urine pregnancy testf
 
X 
 
 
 
 
 
 
 
 
HIV Ab, Hepatitis C Ab, HBsAg, anti-HBcAb 
X 
 
 
 
 
 
 
 
 
  
Interphase FISH and stimulated cytogeneticso 
x 
 
 
 
 
 
 
 
 
IGHV mutation status  
X 
 
 
 
 
 
 
 
 
Flow Cytometryo 
X 
See note l 
 
See note l 
 
Radiographic Studies  
 
 
CT scansg 
X 
 
 
 
 
Xg 
 
 
X 
Exploratory (Correlative) Studies  
 
 
 
Pharmacokinetic samples 
See Section 6.2.3 and Table 7 
 
 
 
Correlative sciences samples 
See Section 6.3.2 and Table 8 
 
See Section 
6.3.2 and 
Table 8 
 
  
Footnotes: 
a. EOT (End of Therapy) visit; should occur 30 ± 7 days after last dose of study drug or prior to the start of a new anticancer therapy, whichever is sooner. 
b. S/RFU (Safety/Response Follow Up); Patients who discontinue treatment for progressive disease and/or use of other CLL/SLL therapy will be followed for 
survival every 24 weeks (± 7 days) for up to 3 years. Patients who discontinue treatment for reasons other than progressive disease, use of other CLL/SLL 
therapy, death, or withdrawal of consent will have response assessments every 12 weeks (± 7 days) until progressive disease occurs. Thereafter they will be 
followed for survival every 24 weeks (± 7 days) for up to 3 years. All patients will discontinue survival follow-up upon completion of 3 years of survival follow-
up, withdrawal of consent, death, or study closure, whichever is the earliest.  For assessment of survival, only a review of patient chart and/or a phone call 
is required. 
c. 100, 200, 250, 300 mg or intermediate doses PO BID according to dose level. Study staff should record clinic-administered doses in the dosing diary and patient 
should record home doses in the dosing diary. 
 
 
e. Serum chemistry should include albumin, alkaline phosphatase, AST, ALT, bicarbonate, BUN, calcium, creatinine, glucose, LDH, magnesium, phosphate, 
potassium, sodium, total bilirubin, total protein, and uric acid. 
f. A urine pregnancy test should be performed with screening in every woman of childbearing potential prior to study entry. 
g. In the CLL/SLL cohorts, CT scans of the chest, abdomen, and pelvis (and neck if involved) should occur at screening and at the post 3, post 6, and post 9 
cycles of therapy assessments (i.e., at or prior to Cycle 4 Day 1, Cycle 7 Day 1, and Cycle 10 Day 1). Patients who continue with drug therapy after 9 cycles 

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Page 100 of 120  
 
 
of treatment should have CT scans, preferably every 12 weeks, or as clinically indicated until disease progression. In the aggressive lymphoma cohort, CT 
scans of the chest, abdomen, and pelvis (and neck if involved) should occur at screening and as clinically indicated or at a minimum of every 24 weeks while 
on treatment. Other image tools for response assessment may be used per RECIL 2017 for lymphomas.   
h. Screening procedures shall be performed within 28 days prior to first dose of study drug. Studies performed at the study site prior to patient signing of 
informed consent can be utilized for screening purposes if obtained within 28 days before first dose of study drug, except in the case of IGHV mutation 
status which are permitted to have been obtained at any time prior to screening. 
i. Beginning in Cycle 2, a window period of +3 days is permitted for assessments and procedures to accommodate public holidays and other emergency or 
unforeseen situations; however, every effort should be made to have visits correspond to Day 1 of the following cycle. 
j.  The ECG will be performed in triplicate at each time-point; all 3 ECG recordings should be completed within 5 minutes. Patient must rest in supine position 
for at least 5 minutes prior to recording an ECG. C1D1, C1D8 and C2D1 ECGs will be recorded 3 hours (± 15 minutes) following dose administration in 
the clinic. ECGs should be recorded prior to any blood draw required at the same time-point [e.g., 3 hours (± 15 minutes) post dose ECGs on C1D1 and 
C1D8 should be recorded prior to 3 hours post dose blood draw]. The QTc from the electrocardiogram should be recorded on the case report form. 
k. Documentation of height is only required at screening.  Weight should be collected at all visits.   
 
 
 
 
 
 
  
 
 
 
 
 
 
  
 
 
 

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APPENDIX 1B: STUDY CALENDAR B COHORTS (ACALBRUTINIB + MS-553) 
  Visit Evaluation Schedule 
Study Cycle (28 days) 
0 
1 
2 
3 
4+ 
EOTa 
S/RFUb 
Cycle dayi 
 
Study Procedure/Assessment 
Screeningh 
1 
8 (Cohort B1 
only) 
 1 (± 3 days) 
1 (±3 days) 
1 (±3 days) 
 
±7 days 
Q12 wks ±7d 
for response or Q24 
wks ±7d for survival 
 
 
 
 
 
 
 
i
n
u
i
 
 
Study Drug/SOC Treatment Dispensation for Cohort B1 and B2 
Continu
 follow-up 
l disease progression, toxicity, or other withdrawal 
 
MS-553 PO BID
c  
 
X 
 
X 
X 
X 
 
 
Acalabrutinib PO BIDo 
 
X 
 
X 
X 
X 
 
 
Procedures  
 
Informed Consent 
X 
 
 
 
 
 
 
 
Confirmation of inclusion/exclusion criteria 
X 
 
 
 
 
 
 
 
Demographics 
X 
 
 
 
 
 
 
 
Medical History and Physical Exam 
X 
X 
X 
X 
X 
X 
X 
X 
Concomitant medication survey 
X 
X 
X 
X 
X 
X 
X 
 
Adverse Events Assessmentr 
 
X 
X 
X 
X 
X 
X 
 
Vital signs; heightk/weight 
X 
X 
X 
X 
X 
X 
X 
X 
WHO/ECOG Performance Status 
X 
X 
X 
X 
X 
X 
X 
X 
Electrocardiogramj 
X 
X 
X 
X 
 
 
 
 
 
 
Flow Cytometryl 
X 
See note m 
See note m 
See note m 
See note m 
See note m 
See note m 
 
 Laboratory Studies  
 
 
 
Complete blood count with differential 
X 
X 
X 
X 
X 
X 
X 
X 
Serum chemistrye
 
X 
X 
X 
X 
X 
X 
X 
 

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Page 102 of 120  
 
 
Study Cycle (28 days) 
0 
1 
2 
3 
4+ 
EOTa 
S/RFUb 
Cycle dayi 
 
Study Procedure/Assessment 
Screeningh 
1 
8 (Cohort B1 
only) 
 1 (± 3 days) 
1 (±3 days) 
1 (±3 days) 
 
±7 days 
Q12 wks ±7d 
for response or Q24 
wks ±7d for survival 
Urinalysis 
X 
 
 
 
 
 
 
 
Urine pregnancy testf
 
X 
 
 
 
 
 
 
 
HIV Ab, Hepatitis C Ab, HBsAg, anti-HBcAb 
X 
 
 
 
 
 
 
 
 
 
Interphase FISH and stimulated cytogeneticsn 
X 
 
 
 
 
 
 
 
IGHV mutation status  
Xh 
 
 
 
 
 
 
 
Radiographic Studies  
 
 
CT scansg 
X 
 
 
 
 
Xg 
 
 
X 
Exploratory (Correlative) Studies  
 
 
 
Pharmacokinetic samples 
 
See Section 6.2.3 and Table 7 
 
 
 
Correlative Science Samples 
See Section 6.3.2 and Table 8 
 
See Section 
6.3.2 and 
Table 8 
 
 
Footnotes: 
a. EOT (End of Therapy) visit; should occur 30 ± 7 days after last dose of study drug or prior to the start of a new anticancer therapy, whichever is sooner. 
b. S/RFU (Safety/Response Follow Up); Patients who discontinue treatment for progressive disease and/or use of other CLL/SLL therapy will be followed for 
survival every 24 weeks (± 7 days) for up to 3 years. Patients who discontinue treatment for reasons other than progressive disease, use of other CLL/SLL 
therapy, death, or withdrawal of consent will have response assessments every 12 weeks (± 7 days) until progressive disease occurs. Thereafter they will be 
followed for survival every 24 weeks (± 7 days) for up to 3 years. All patients will discontinue survival follow-up upon completion of 3 years of survival follow-
up, withdrawal of consent, death, or study closure, whichever is the earliest.  For assessment of survival, only a review of patient chart and/or a phone call 
is required. 
c. 100, 200, 250, or 300 mg PO BID according to dose level. Study staff should record clinic-administered doses in the dosing diary and patient should record 
home doses in the dosing diary. 
 
 
  
e. Serum chemistry should include albumin, alkaline phosphatase, AST, ALT, bicarbonate, BUN, calcium, creatinine, glucose, LDH, magnesium, phosphate, 
potassium, sodium, total bilirubin, total protein, and uric acid. 
f. A urine pregnancy test should be performed with screening in every woman of childbearing potential prior to study entry. 

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g. In the CLL/SLL cohorts, CT scans of the chest, abdomen, and pelvis (and neck if involved) should occur at screening and at the post 3, post 6, and post 9 
cycles of therapy assessments (i.e., at or prior to Cycle 4 Day 1, Cycle 7 Day 1, and Cycle 10 Day 1). Patients who continue with drug therapy after 9 cycles 
of treatment should have CT scans, preferably every 12 weeks, or as clinically indicated until disease progression. In the aggressive lymphoma cohort, CT 
scans of the chest, abdomen, and pelvis (and neck if involved) should occur at screening and as clinically indicated or at a minimum of every 24 weeks while 
on treatment. 
h. Screening procedures shall be performed within 28 days prior to first dose of study drug. Studies performed at the study site prior to patient signing of 
informed consent can be utilized for screening purposes if obtained within 28 days before first dose of study drug, except in the case of IGHV mutation 
status which are permitted to have been obtained at any time prior to screening. 
i. Beginning in Cycle 2, a window period of +3 days is permitted for assessments and procedures to accommodate public holidays and other emergency or 
unforeseen situations; however, every effort should be made to have visits correspond to Day 1 of the following cycle.  
j.  The ECG will be performed in triplicate at each time-point; all 3 ECG recordings should be completed within 5 minutes. Patient must rest in supine position 
for at least 5 minutes prior to recording an ECG. C1D1, C1D8 and C2D1 ECGs will be recorded 3 hours (± 15 minutes) following MS-553 administration 
in the clinic. ECGs should be recorded prior to any blood draw required at the same time-point [e.g., 3 hours (± 15 minutes) post dose ECGs on C1D1 and 
C1D8 should be recorded prior to 3 hours post dose blood draw]. The QTc from the electrocardiogram should be recorded on the case report form. 
k. Documentation of height is only required at screening.  Weight should be collected at all visits.  
 
 
 
  
 
 
n. 
 
 
o. Acalabrutinib administered according to institutional standard of care. It is recommended that acalabrutinib be taken 90 minutes (but at least 1 hour) prior 
to the time when MS-553 is taken. 
 
 
 
 
 
 
 

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APPENDIX 1C: STUDY CALENDAR C COHORTS (TRIPLET REGIMEN MS-553 + RITUXIMAB + VENETOCLAX) 
Visit Evaluation Schedule 
Study Cycle (28 days) 
0 
1 
2 
3 
4+ 
EOTa 
S/RFUb 
Cycle dayi 
 
Study Procedure/Assessment 
Screeningh 
1 
2 
8q 
 1  
(± 3 days) 
1 
 (±3 
days) 
2 
8 
15 
22 
1 
(±3 days) 
±7 days 
Q12 wks ±7d 
for response or Q24 wks 
±7d for survival 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
MS-553 PO BID
c, s  
 
X 
 
 
X 
X 
 
 
 
X 
 
 
Venetoclax PO QD (5 week ramp up 
dose)r,p 
 
 
 
 
 
X 
X 
X 
X 
X 
Xp 
 
 
Anti-CD20 mAb (rituximab) p 
 
X 
 
 
X 
X 
 
 
 
Xp 
 
 
Procedures  
 
Informed Consent 
X 
 
 
 
 
 
 
 
 
 
 
 
Confirmation of inclusion/exclusion 
criteria 
X 
 
 
 
 
 
 
 
 
 
 
 
Demographics 
X 
 
 
 
 
 
 
 
 
 
 
 
Medical History and Physical Exam 
X 
X 
X 
Xq 
X 
X 
 
X 
X 
X 
X 
X 
X 
Concomitant medication survey 
X 
X 
X 
Xq 
X 
X 
X 
X 
X 
X 
X 
X 
 
Adverse Events Assessmento 
 
X 
X 
Xq 
X 
X 
X 
X 
X 
X 
X 
X 
 
Vital signs; heightk/weight 
X 
X 
X 
Xq 
X 
X 
 
X 
X 
X 
X 
X 
X 
WHO/ECOG Performance Status 
X 
X 
X 
Xq 
X 
X 
 
 
 
 
X 
X 
X 
Electrocardiogramj 
X 
X 
 
Xq 
X 
 
 
 
 
 
 
 
Flow Cytometryl 
X 
See 
note 
m
See 
note 
m
See 
note 
m
See note m 
See 
note m 
See note m 
See note 
m 
See note m 
 
 Laboratory Studies  
 
 
 
Complete blood count with 
differential 
X 
X 
X 
Xq 
X 
X 
X 
X 
X 
X 
X 
X 
X 

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Study Cycle (28 days) 
0 
1 
2 
3 
4+ 
EOTa 
S/RFUb 
Cycle dayi 
 
Study Procedure/Assessment 
Screeningh 
1 
2 
8q 
 1  
(± 3 days) 
1 
 (±3 
days) 
2 
8 
15 
22 
1 
(±3 days) 
±7 days 
Q12 wks ±7d 
for response or Q24 wks 
±7d for survival 
Serum chemistrye
 
X 
X 
X 
Xq 
X 
X 
X 
Xr 
Xr 
Xr 
X 
X 
 
Urinalysis 
X 
 
 
 
 
 
 
 
 
 
 
 
Urine pregnancy testf
 
X 
 
 
 
 
 
 
 
 
 
 
 
HIV Ab, Hepatitis C Ab, HBsAg, anti-
HBcAb 
X 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Interphase FISH and stimulated 
cytogeneticsn 
X 
 
 
 
 
 
 
 
 
 
 
 
IGHV mutation statush  
XX 
 
 
 
 
 
 
 
 
 
 
 
Radiographic Studies  
 
CT scansg 
X 
 
 
 
 
Xq 
 
 
 
 
Xg 
 
X 
Exploratory (Correlative) Studies  
 
Pharmacokinetic samples 
See Section 6.2.3 and Table 7 
 
 
Correlative Science samples 
See Section 6.3.2 and Table 8 
See Section 6.3.2 
and Table 8 
 
 
Footnotes: 
a. EOT (End of Therapy) visit; should occur 30 ± 7 days after last dose of study drug or prior to the start of a new anticancer therapy, whichever is sooner. 
b. S/RFU (Safety/Response Follow Up); Patients who discontinue treatment for progressive disease and/or use of other CLL/SLL therapy will be followed for 
survival every 24 weeks (± 7 days) for up to 3 years. Patients who discontinue treatment for reasons other than progressive disease, use of other CLL/SLL 
therapy, death, or withdrawal of consent will have response assessments every 12 weeks (± 7 days) until progressive disease occurs. Thereafter they will be 
followed for survival every 24 weeks (± 7 days) for up to 3 years. All patients will discontinue survival follow-up upon completion of 3 years of survival follow-
up, withdrawal of consent, death, or study closure, whichever is the earliest.  For assessment of survival, only a review of patient chart and/or a phone call 
is required. 
c. 100, 200, 250, or 300 mg PO BID according to dose level. Study staff should record clinic-administered doses in the dosing diary and patient should record 
home doses in the dosing diary. 
 
 

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e. Serum chemistry should include albumin, alkaline phosphatase, AST, ALT, bicarbonate, BUN, calcium, creatinine, glucose, LDH, magnesium, phosphate, 
potassium, sodium, total bilirubin, total protein, and uric acid. 
f. A urine pregnancy test should be performed with screening in every woman of childbearing potential prior to study entry. 
g. In the CLL/SLL cohorts, CT scans of the chest, abdomen, and pelvis (and neck if involved) should occur at screening and at the post 2, post 6, and post 9 
cycles of therapy assessments (i.e., at or prior to Cycle 3 Day 1, Cycle 7 Day 1, and Cycle 10 Day 1). Patients who continue with drug therapy after 9 cycles 
of treatment should have CT scans, preferably every 12 weeks, or as clinically indicated until disease progression. In the aggressive lymphoma cohort, CT 
scans of the chest, abdomen, and pelvis (and neck if involved) should occur at screening and as clinically indicated or at a minimum of every 24 weeks while 
on treatment. 
h. Screening procedures shall be performed within 28 days prior to first dose of study drug. Studies performed at the study site prior to patient signing of 
informed consent can be utilized for screening purposes if obtained within 28 days before first dose of study drug, except in the case of IGHV mutation 
status which are permitted to have been obtained at any time prior to screening. 
i. Beginning in Cycle 2, a window period of +3 days is permitted for assessments and procedures to accommodate public holidays and other emergency or 
unforeseen situations; however, every effort should be made to have visits correspond to Day 1 of the following cycle. 
j.  The ECG will be performed in triplicate at each time-point; all 3 ECG recordings should be completed within 5 minutes. Patient must rest in supine position 
for at least 5 minutes prior to recording an ECG. C1D1, C1D8 and C2D1 ECGs will be recorded 3 hours (± 15 minutes) following MS-553 administration 
in the clinic. ECGs should be recorded prior to any blood draw required at the same time-point [e.g., 3 hours (± 15 minutes) post dose ECGs on C1D1 and 
C1D8 should be recorded prior to 3 hours post dose blood draw]. The QTc from the electrocardiogram should be recorded on the case report form. 
k. Documentation of height is only required at screening.  Weight should be collected at all visits.  
l. Flow cytometry will be performed at screening on both bone marrow (when available) and peripheral blood samples (all patients). If a bone marrow 
aspirate/biopsy is performed to confirm a CR, flow cytometry should be performed on the bone marrow sample. 
 
 
 
 
 
 
 
 
p. Venetoclax should be taken orally once daily with food and water, per the package insert ≥ 6 hr after the morning dose of MS-553. Dosing Schedule for 
Ramp-Up Phase in Patients with CLL/SLL.VENETOCLAX Daily Dose is according to standard of care, starting with Cycle 3: Week 1 is as follows: 20 mg; Week 
2 dose is 50 mg; Week 3 dose is 100 mg; Week 4 dose is 200 mg; Week 5 and beyond dose is 400 mg.  When venetoclax is combined with rituximab, 
venetoclax is discontinued after 24 months of therapy (last dose on C26D28) and rituximab is discontinued after 6 cycles of administration, to ensure 
consistency with the standard of care. 
q. CT scans can be performed prior to starting venetoclax treatment to allow for restaging, allowing outpatient therapy if TLS risk is reduced.  
r. While day 9, Day 16 and Day 23 are not on the study calendar, blood chemistry monitoring at 24 hour post venetoclax dose is recommended for the first 
dose of 50mg for all patients (i.e., day 9) and for the subsequent ramp-up doses for high tumor burden patients (i.e., day 16 and day 23). Please refer to 
venetoclax package insert.  

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s. For patients who have achieved complete responses with the treatment of MS-553, venetoclax, and rituximab, assessments of uMRD in a central lab will 
be performed in a fashion consistent with IWCLL 2018 guideline. If uMRD in both peripheral blood and bone marrow is confirmed via a central lab, MS-553 
may be discontinued at the discretion of the investigators after completion of venetoclax administration as defined in its package label.  
 
 
 
 
 
 

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APPENDIX 2A: MS-553 RECOMMENDED DOSE MODIFICATIONS AND DOSE 
DELAYS 
Recommended dose modifications for MS-553 for Grade ≥3 toxicities at least possibly related to MS-553 
Worst toxicity Grade* (value) 
During a cycle of therapy 
Hematology 
• Grade 4 neutropenia without fever (ANC 
<0.5 x 109/L), unless the neutropenia was 
present at the time of study entry  
and/or 
• iwCLL 2018 Grade 3 or above 
thrombocytopenia: 
Grade^  
Decrease in 
platelets† from 
baseline value, %  
0  
No change to 10  
1  
11-24  
2  
25-49  
3  
50-74  
4  
≥75  
^Grades: 1, mild; 2, moderate; 3, severe; 4, 
life-threatening; 5, fatal. Death occurring as 
a result of toxicity at any level of decrease 
from baseline will be recorded as grade 5. 
 
†Platelet counts must be below normal 
levels for grades 1-4. If, at any level of 
decrease the platelet count is <20 × 109/L, 
this will be considered grade 4 toxicity 
unless a severe or life-threatening decrease 
in the initial platelet count (e.g., 20 × 109/L) 
was present at baseline, in which case the 
patient is not evaluable for toxicity 
referable to platelet counts. 
Hold MS-553 until ANC is ≥ 0.5 x 109/L and 
until platelets recover to Grade 2 or lower 
per iwCLL 2018 grading.  
If treatment delay is ≤ 7 days, restart at same 
dose. 
If treatment delay is > 7 days but ≤ 28 days, 
restart at lower dose.  
If treatment delay > 28 days, discontinue 
study treatment 

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Grade 3 Febrile Neutropenia (ANC 
<1.0 x 109/L with fever) 
If ANC at study entry was ≥ 1.0 x 109/L: 
• Hold MS-553 until ANC is ≥ 1.0 x 109/L 
If ANC at study entry was < 1.0 x 109/L due to 
bone marrow involvement: 
• Hold MS-553 until ANC returns to 
pretreatment level 
If treatment delay is ≤ 7 days, restart at same 
dose 
If treatment delay is >7 days but ≤ 28 days, 
restart at lower dose.  
If treatment delay >28 days, discontinue study 
treatment 
Nausea, Vomiting or Diarrhea with maximal prophylaxis 
Grade 3 
Hold MS-553 dosing until improved to grade 
1 or baseline, then restart at lower dose level 
for MS-553 
Grade 4 
Hold MS-553 dosing until improved to grade 
1 or baseline. If improves in <24 hours, 
restart at lower dose for MS-553 
If grade 4 GI toxicity persists > 24 hours 
despite optimal use of SoC, discontinue 
treatment with MS-553. 
All other Toxicities 
≥ Grade 3 
Hold MS-553 until improved to ≤ Grade 1 or 
baseline. If treatment delay is < 7 days, restart 
at same dose or at a lower dose level upon 
agreement between the investigator and 
Sponsor. If treatment delay is >7 days but ≤ 28 
days, restart at lower dose level. If treatment 
delay >28 days, discontinue study treatment. 

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*Common Toxicity Criteria for Adverse Events (CTCAE Version 5.0)  

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APPENDIX 2B: DOSE MODIFICATIONS OF ACALABRUTINIB 
Recommended dose modifications for acalabrutinib for Grade 3 or greater adverse reactions 
are outlined below. Please refer to the acalabrutinib prescribing information for more specific 
details. 
 
Event 
Adverse Reaction 
Occurrence  
Dose Modification  
(Starting dose = 100 mg approximately every 
12 hours 
Grade 3 or greater non-
hematologic toxicities,  
Grade 3 thrombocytopenia 
with bleeding,  
Grade 4 thrombocytopenia  
or 
Grade 4 neutropenia lasting 
longer than 7 days 
First and Second 
Interrupt acalabrutinib dosing.  
Once toxicity has reserved to Grade 1 or 
baseline, acalabrutinib may be resumed at 
100 mg approximately every 12 hours. 
Third  
 
Interrupt acalabrutinib dosing.  
Once toxicity has reserved to Grade 1 or 
baseline, acalabrutinib may be resumed at a 
reduced frequency of 100 mg once daily.  
Fourth 
Discontinue acalabrutinib 
 
 
 
 

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APPENDIX 2C: DOSE MODIFICATIONS OF VENETOCLAX AND RITUXIMAB 
Dose modifications or hold of rituximab can be made by the treating physicians per institutional 
standard.  
The recommended dosage modifications for venetoclax for adverse reactions are provided in 
the table below.  For patients who have had a dosage interruption greater than 1 week during 
the first 5 weeks of ramp-up phase or greater than 2 weeks after completing the ramp-up 
phase, reassess for risk of TLS to determine if re-initiation with a reduced dose is necessary 
(e.g., all or some levels of the dose ramp-up schedule). 
Recommended Venetoclax Dosage Modifications for Adverse Reactionsa in CLL/SLL 
Adverse Reaction 
Occurrence 
Dosage Modification 
Tumor Lysis Syndrome 
Blood chemistry changes or 
symptoms suggestive of TLS 
Any 
Withhold the next day’s dose. If 
resolved within 24 to 48 hours 
of last dose, resume at same 
dose. 
For any blood chemistry 
changes requiring more than 48 
hours to resolve, resume at 
reduced dose  
For any events of clinical TLSb, 
resume at reduced dose 
following resolution  
Non-Hematologic Adverse Reactions 
Grade 3 or 4 nonhematologic 
toxicities 
1st occurrence 
Interrupt venetoclax.  
Upon resolution to Grade 1 or 
baseline level, resume 
venetoclax at the same dose. 
2nd and subsequent 
occurrences 
Interrupt venetoclax.  
Follow dose reduction 
guidelines when resuming 
treatment with venetoclax after 
resolution. A larger dose 
reduction may occur at the 
discretion of the physician. 

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Hematologic Adverse Reactions 
Grade 3 neutropenia with 
infection or fever; or Grade 4 
hematologic toxicities (except 
lymphopenia) 
1st occurrence 
Interrupt venetoclax.  
Upon resolution to Grade 1 or 
baseline level, resume 
venetoclax at the same dose. 
2nd and subsequent 
occurrences 
Interrupt venetoclax.  
Follow dose reduction 
guidelines when resuming 
treatment with venetoclax after 
resolution. A larger dose 
reduction may occur at the 
discretion of the physician. 
Consider discontinuing venetoclax for patients who require dose reductions to less than 100 mg for 
more than 2 weeks.  
aAdverse reactions were graded using NCI CTCAE version 5.0.  
bClinical TLS was defined as laboratory TLS with clinical consequences such as acute renal failure, 
cardiac arrhythmias, or sudden death and/or seizures. 
The recommended dose reductions for venetoclax for adverse reactions are provided in the 
table below. 
Recommended Dose Reduction for Adverse Reactions for venetoclax in CLL/SLL 
Dose at Interruption, mg 
Restart Dose, mga 
400 
300 
300 
200 
200 
100 
100 
50 
50 
20 
20 
10 
aDuring the ramp-up phase, continue the reduced dose for 1 week before increasing the dose. 

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▪ 
 
 
 
 
 
 
  
 
 
 
 

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▪ 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
     
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

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APPENDIX 3: LIST OF CO-MEDICATIONS TO AVOID 
A. Strong and moderate CYP3A inhibitors, inducers and sensitive and moderately sensitive 
substrates  
CYP3A4 Inducers 
apalutamide 
macrolide antibiotics 
primidone 
bosentan 
mitotane 
rifabutin 
carbamazepine 
phenobarbital 
rifampin 
dexamethasone 
phenylbutazone 
rifapentine 
efavirenz 
phenytoin 
sulfinpyrazone 
enzalutamide 
prednisone 
St. John’s wort 
glucocorticoids 
 
 
CYP3A4 Inhibitors 
amprenavir 
elvitegravir 
paritaprevir 
anastrozole 
erythromycin 
paroxetine 
aprepitant 
fluconazole 
posaconazole 
boceprevir 
fluvoxamine 
propranolol 
canazol 
grapefruit juice 
quinidine 
celavirdine 
idelalisib 
quinine 
cimetidine 
imatinib 
ranitidine 
ciprofloxacin 
indinavir 
ritonavir 
crizotinib 
itraconazole 
saquinavir 
clarithromycin 
ketoconazole 
sertraline 
clotrimazole 
lopinavir 
telithromycin 
cobicistat 
mibefradil 
tofisopam 
cyclosporine 
miconazole 
troglitazone 
dasabuvir 
nefazodone 
troleandomycin 
danoprevir 
nelfinavir 
verapamil 
diethyldithiocarbamate  
nevirapine 
voriconazole 
diltiazem 
norfloxacin 
zafirlukast 
dronedarone 
ombitasvir 
 
CYP3A5 Inducers 
phenobarbital 
primidone 
troleandomycin  
phenytoin 
rifampin 
 
CYP3A5 Inhibitors 
clotrimazole 
metronidazole 
troleandomycin 
ketoconazole 
miconazole 
CYP3A Sensitive Substrates  
alfentanil 
everolimus 
saquinavir 
avanafil 
felodipine 
sildenafil 
buspirone 
ibrutinib 
simvastatin 
budesonide 
indinavir 
sirolimus 

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conivaptan 
lomitapide 
tacrolimus 
darifenacin 
lovastatin 
ticagrelor 
darunavir 
lurasidone 
tipranavir 
dasatinib 
maraviroc 
tolvaptan 
dronedarone 
midazolam 
triazolam 
ebastine 
naloxegol 
vardenafil 
eletriptan 
nisoldipine 
 
eplerenone 
quetiapine 
 
CYP3A Moderate Sensitive Substrates 
alprazolam 
colchicine 
rilpivirine 
aprepitant 
eliglustat 
tadalafil 
atorvastatin 
pimozide 
 
 
Source for CP3A information: 
 
Tatro DS, Drug Interaction Facts: The Authority on Drug Interactions. Wolters Kluwer 
Health, 2012. 
 
FDA Drug Development and Drug Interactions: Table of Substrates, Inhibitors and 
Inducers.  
 
Web link Accessed 20 December 2019: 
https://www.fda.gov/drugs/drug-interactions-labeling/drug-development-and-drug-
interactions-table-substrates-inhibitors-and-inducers 
 
 
B. Drugs that prolong QTc interval 
 
Source for QTc drug list: 
Woosley, RL and Romero, KA, www.Crediblemeds.org, QTdrugs List, Accession Date, 
AZCERT, Inc. 1822 Innovation Park Dr., Oro Valley, AZ 85755  
 
Web link Accessed 23 August 2019: 
https://www.crediblemeds.org/ 
 
 

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APPENDIX 4: NCI CTCAE V 5.0 AND IWCLL GUIDELINES (2008 & 2018) 
 
National Cancer Institute Common Toxicity Criteria for Adverse Events v 5.0 
https://ctep.cancer.gov/protocoldevelopment/electronic_applications/ctc.htm#ctc_50 
 
iwCLL guidelines for diagnosis, indications for treatment, response assessment, and supportive 
management of CLL.  Blood (2018) 131 (25): 2745–2760. 
https://doi.org/10.1182/blood-2017-09-806398 
 
 

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APPENDIX 5: CLL/SLL RESPONSE ASSESSMENTS 
 
A. International Workshop on Chronic Lymphocytic Leukemia Guideline (2008) 
Guidelines for the diagnosis and treatment of chronic lymphocytic leukemia: a report 
from the International Workshop on Chronic Lymphocytic Leukemia updating the 
National Cancer Institute Working Group 1996 guidelines Blood 2008 111:5446-5456; 
https://doi.org/10.1182/blood-2007-06-093906  
 
 
B. International Working Group consensus response evaluation criteria in lymphoma 
(RECIL 2017) Ann Oncol. 2017 Jul; 28(7): 1436–1447. 
     https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5834038