A Randomized, Double-Blind, Placebo-Controlled Multiple-Center, Efficacy and Safety Study of ZYN002 Administered as a Transdermal Gel to Children and Adolescents With Fragile X Syndrome
Phase: PHASE2
Conditions: Fragile X Syndrome
Sponsor: Zynerba Pharmaceuticals, Inc.

Protocol ZYN2-CL-016.05 
26 April 2020 
 
 
ZYN2-CL-016 
A Randomized, Double-Blind, Placebo-Controlled Multiple-Center, Efficacy and 
Safety Study of ZYN002 Administered as a Transdermal Gel to Children and 
Adolescents with Fragile X Syndrome – CONNECT-FX 
Clinical study Of caNNabidiol in childrEn and adolesCenTs with Fragile X 
(CONNECT-FX) 
  
Protocol Number: 
ZYN2-CL-016.05 
Original Protocol Date: 
5 March 2018 
Protocol Amendment .01 Date: 
24 May 2018 
Protocol Amendment .02 Date: 
19 September 2018 
Protocol Amendment .03 Date: 
22 January 2019 
Protocol Amendment .04 Date: 
30 March 2019 
Protocol Amendment .05 Date: 
26 April 2020 
 
IND Number: 
130876 
Sponsor: 
Zynerba Pharmaceuticals Pty., Ltd. 
 
2 Riverside Quay 
 
Southbank, VIC 3006 Australia 
 
 
 
Zynerba Pharmaceuticals, Inc. 
 
80 W. Lancaster Ave., Suite 300 
 
Devon, PA 19333 United States of America 
 
Medical Monitors: 
 
 
 
 
CONFIDENTIAL INFORMATION 
The information contained in this document is the property of Zynerba Pharmaceuticals Pty., Ltd. 
(Zynerba) and Zynerba Pharmaceuticals Inc. (Zynerba) and is confidential.  It is intended solely for 
the use of Zynerba employees and contracting consultants and regulatory agencies.  Reproduction or 
disclosure of the information contained herein in whole or in part is forbidden to persons not involved 
in the clinical investigation of Zynerba, unless such persons are bound by a confidentiality agreement 
with Zynerba. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
2 
1. 
INVESTIGATOR’S AGREEMENT 
I have received and read the Investigator’s Brochure Edition 7 dated 22 May 2019 for ZYN002.  
I have read the ZYN2-CL-016.05 protocol dated 26 April 2020 and agree to conduct the study as 
outlined.  I agree to maintain the confidentiality of all information received or developed in 
connection with this protocol.  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Printed Name of Investigator 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Signature of Investigator 
 
 
 
 
 
 
 
 
Date 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
3 
PROCEDURES IN CASE OF EMERGENCY 
 
Table 1:  Emergency Contact Information 
Role in Study 
Name 
Email Address and Telephone Number 
Responsible Physician 
 
 
 
 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
4 
2. 
SYNOPSIS 
Name of Sponsor/Company: Zynerba Pharmaceuticals Pty., Ltd. 
Zynerba Pharmaceuticals, Inc. 
Name of Investigational Product (IP): ZYN002 
Name of Active Ingredient: Cannabidiol (CBD) 
Title of Study:  A Randomized, Double-Blind, Placebo-Controlled, Multiple-Center, Efficacy and 
Safety Study of ZYN002 Administered as a Transdermal Gel to Children and Adolescents with Fragile 
X Syndrome - CONNECT-FX 
Study Centers: Australia, Canada, New Zealand, and the United States of America 
Study Period (years): 
Estimated date first patient enrolled: June 2018  
Estimated date last patient enrolled: January 2020 
Estimated date last patient completed: May 2020 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
5 
Objectives:  
Primary: 
To evaluate the efficacy of ZYN002 administered as a transdermal gel formulation, for up to 12 weeks, 
in patients ages 3 to < 18 years, in the treatment of symptoms of Fragile X Syndrome (FXS).  The 
primary endpoint is the change from Baseline to Week 12 in the Aberrant Behavior Checklist-
Community FXS Specific (ABC-CFXS) Social Avoidance subscale.  
Secondary: 
To further evaluate the efficacy of ZYN002 in the treatment of symptoms of FXS. 
To evaluate the safety of ZYN002 in the treatment of symptoms of FXS. 
To evaluate CBD, CBD metabolite and tetrahydrocannabinol (THC) exposure. 
Methodology:  
This is a randomized, double-blind, placebo-controlled, multiple-center study, to assess the efficacy 
and safety of CBD administered as ZYN002, a transdermal gel, for the treatment of child and 
adolescent patients with FXS.  Male and female patients with FXS will be treated for 12 weeks with a 
 single-blind placebo lead-in preceding the 12-week double-blind treatment period.  
Approximately 204 male and female patients, ages 3 to < 18 years, will be randomized 1:1 to either 
trial drug or placebo.  Randomization will be stratified by gender, weight category and region.  
Site-specific supplemental protocols may be implemented in a subset of ZYN2-CL-016 patients. 
Study Screening: 
Prior to any Screening Procedures being performed, the parent/caregiver will provide written informed 
consent and, if applicable, the patient will provide assent.  During the Screening Period, the site staff 
will review the eligibility criteria, review any medications including over-the-counter (OTC) 
medications the patient is taking, obtain the patient’s medical history including their Fragile X 
Diagnosis, any seizure history and demographics, check their vital signs, perform an electrocardiogram 
(ECG), assess Tanner Stage, perform a physical and neurological exam and a skin assessment, obtain 
blood and urine for analysis, and administer assigned scales.  Blood samples will be taken for 
hematology and chemistry testing, testosterone (T) testing (males only), pregnancy testing (if 
applicable) and Cytosine Guanine Guanine (CGG) repeat analysis.  Patients will also have a blood 
sample drawn for plasma levels of CBD and THC testing, and adjunctive anti-epileptic drugs (AEDs) 
(for patients using AEDs).  Urine will be collected for a urinalysis and drug screen.  The scales 
administered include the ABC-CFXS, Autism Diagnostic Observation Schedule®-2 (ADOS®-2) ( 
ADOS®-2 will not be administered at Screening if it has been administered in the prior 6 months and 
the results are available), Clinical Global Impression-Severity (CGI-S), Columbia-Suicide Severity 
Rating Scale – Children’s version (C-SSRS), Marijuana Withdrawal Checklist – Short Form (MWC, 
Behavior Checklist), Penn Physician Withdrawal Checklist (PWC-20) and the Vineland Adaptive 
Behavior Scales™ 3rd Edition (VABS-3).   

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
6 
The parent/caregiver will also be asked the following question “What are the five behavioral, 
emotional, or social problems that most impacted your son/daughter and his/her family in 
approximately the past year?”  This is referred to as the “Qualitative Caregiver reported Behavioral 
Problems Survey” in the Schedule of Assessments, Table 3.  Optionally, if an intelligence quotient 
(IQ) test was performed previously those results may be provided to the Sponsor. 
 Single Blind Placebo Lead-In Period:  
Following the Screening period, eligible patients will start a 
 Placebo Lead-In period.  At 
Visit 2, parents/caregivers will be instructed on proper application of the study gel (active/placebo gel). 
  
Parents/caregivers will apply all trial drug to clean, dry, intact skin, thoroughly massaging it into the 
right and/or left upper arms and shoulders until the area is dry.  The study gel will be rubbed in 
completely and must be dry prior to dressing.  
 
 
Parents/caregivers will use gloves supplied by the Sponsor to apply the study gel.  If redness occurs at 
the application site, the parent (after consultation with the Investigator) may switch the application site 
temporarily to the upper thighs.  Parents/caregivers will complete the Caregiver Global Impression of 
Severity scale at Visit 2. 
12-Week Double Blind Treatment Period 
At Visit 3, patients will be randomized to receive either ZYN002 or placebo.  Patients will qualify for 
randomization if they continue to meet the inclusion criteria and none of the exclusion criteria for the 
study.  Patients eligible for randomization will be either: 
• 
Patients with an ABC-CFXS 
 score 
 on Study Day 1 prior to 
randomization, who have
 
OR  
• 
Patients with an ABC-CFXS 
 score of 
 with an ABC-CFXS 
 
score 
 on Study Day 1 prior to randomization, who have
   
All inclusion and exclusion criteria must be reviewed for eligibility for randomization. 
In a blinded fashion, ZYN002-treated patients who weigh < 35 kg will receive 125 mg CBD Q12H 
(every 12 hours) (± 2 hours); for a total daily dose of 250 mg CBD.  Patients who weigh > 35 kg will 
receive 250 mg CBD Q12H (+2 hours); for a total daily dose of 500 mg CBD.  All patients will remain 
on their assigned dose during the 12 weeks of the treatment phase of the study.  Study visits will occur 
at Week 4 (Visit 4), Week 8 (Visit 5) and Week 12 (Visit 6).  Patients who have successfully 
completed the 12 weeks of the double-blind study and have been at least 90% compliant with the trial 
drug and visits will have the option to enroll in an Open-Label Extension Study (OLE).  A weekly 
Telephone Follow-Up call will occur for four weeks following the last dose of trial drug for patients 
who do not enter the OLE. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
7 
Patients and parents/caregivers will be required to visit the clinic at Week -2 (Visit 2), Study Day 
1/Week 1 (Visit 3), Week 4 (Visit 4), Week 8 (Visit 5), and Week 12 (Visit 6) for vital signs, ECG, 
concomitant medication check, physical and neurological exams, Tanner Stage assessment, skin check 
examination, adverse event (AE) review including seizure assessment if applicable, and completion of 
the following questionnaires and scales: ABC-CFXS, Anxiety Depression and Mood Scale (ADAMS), 
Clinical Global Impression-Improvement (CGI-I), CGI-S, C-SSRS, Pediatric Quality of Life 
Inventory™ – Family Impact (Family Impact PedsQL™), MWC and PWC-20. 
In addition, parents/caregivers will complete a Caregiver Global Impression of Severity at Visit 3, and 
Visit 6/ET.  Parents/caregivers will also complete a Caregiver Global Impression of Change at 
Visit 6/ET. 
End of Study (EOS) / Early Termination Visit (ET): 
Patients who have successfully completed through Week 12 of the protocol and have been at least 90% 
compliant with the trial drug and study visits will have the opportunity to continue in an OLE Study.   
Patients not on AEDs who are not continuing in the OLE study or are prematurely discontinuing will 
complete the EOS procedures at Visit 6/ET.  See Table 3 Schedule of Assessments for procedures at 
Visit 6/ET. 
Patients taking concomitant AEDs who are not continuing in the OLE study or are prematurely 
discontinuing will taper down their study dose in the following manner at Visit 6/ET: 
• For patients weighing < 35 kg (250 mg daily dose), the dose of trial drug will be reduced, in a 
blinded manner, to a total daily dose of 125 mg ZYN002 or 2.98 g placebo gel each day for one 
week (one sachet each morning), after which time the patients will discontinue from the study and 
attend the EOS Visit.  See Table 3 Schedule of Assessments for procedures at the EOS Visit.  The 
parent/caregiver will be contacted weekly during the Taper Period and complete the MWC and 
PWC-20. 
• For those patients > 35 kg (500 mg daily dose), the dose of trial drug will be reduced, in a blinded 
manner over two weeks: during the first week of taper the dose will be reduced to 125mg Q12H; 
(ZYN002 or placebo) (+2 hours); total daily dose of 250 mg, followed by a second week of taper 
from 250 mg total daily dose to a total daily dose of 125 mg ZYN002 or 2.98g placebo gel each 
day (one sachet each morning).  After the taper, patients will discontinue from the study and 
attend the EOS Visit.  See Table 3 Schedule of Assessments for procedures at the EOS Visit.  The 
parent/caregiver will be contacted weekly during the Taper Period and complete the MWC and 
PWC-20. 
 
 
Patients who do not enter the OLE study or who discontinue early will have a Telephone Follow-Up 
call weekly for four weeks following each patient’s last dose of trial drug.  The parent/caregiver will 
be contacted weekly via the telephone during the Follow-Up and complete the MWC and PWC-20.   

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
8 
Safety Monitoring: 
Patient safety will be monitored during study visits using standard measures, including physical and 
neurological exams, Tanner Stage assessment, examination of skin at application sites for irritation, 
vital signs (including oral, infrared forehead or tympanic temperature), 12-lead ECGs, the C-SSRS, the 
MWC, the PWC-20, safety laboratory tests, urinalysis, seizure assessment and AE monitoring.  
Misuse, Abuse, and Diversion Drug Event Reporting System (MADDERS) will be used to 
systemically capture and adjudicate abuse-related events.  At the discretion of the investigator, patients 
will be allowed a short acting sedative (such as midazolam) to assist with the collection of blood 
samples and ECG’s. 
At Screening, patients will have an epilepsy diagnosis and seizure classification confirmed by the 
Investigator.  The parents/caregivers of patients with a current epilepsy and/or seizure diagnosis will be
asked at each study visit if there has been any increase in frequency or severity of the patient’s 
seizures.  If an increase in frequency or severity is noted, this will be recorded as an adverse event 
(AE) in the case report form (CRF). 
Parents/caregivers will be provided a diary to complete a daily skin check examination.  Every evening 
they will record the skin check score in the daily skin check diary.  When skin redness is noted, 
parents/caregivers should apply the gel to a non-red area of the upper arms and shoulders.  If the skin 
check score is higher than ‘2’(moderate erythema) at any time, the parent/caregiver will contact the 
study site to determine if an Unscheduled Visit is required.  The Investigator will use discretion in 
suspending dosing for patients with a skin check score of ‘4’ but will, in all cases, immediately (within 
24 hours) complete an adverse event report and contact their study Clinical Research Associate (CRA) 
and the Zynerba Medical Monitor.   A de-identified photograph of the skin finding (or area) of interest 
may be taken after consultation with and approval of the Sponsor.  Digital photographs will be retained 
for information purposes only.  In place of suspending the dosing due to skin irritation, the Investigator 
may instruct the parent/caregiver to temporarily change the application site to the upper thigh.  
Blood Samples of AEDs, CBD, and THC: 
Blood samples for plasma levels of CBD and THC will be collected at the Screening Visit, Visit 4, and 
Visit 6/ET.  Plasma may also be analyzed for CBD metabolite concentrations.  In addition, blood 
samples for adjunctive AED blood levels (for patients taking AEDs) will be collected at Screening, 
Visit 4, and Visit 6/ET.  The time of blood sample collection, as well as the time of last AED and trial 
drug dose, will be recorded.  Plasma samples will be analyzed
 
  Plasma samples for adjunctive AEDs will be analyzed through a 
commercial laboratory.   
Number of Patients (planned): Approximately 204 male and female patients will be randomized 1:1 
to either trial drug or placebo.   

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
9 
Diagnosis and Criteria for Inclusion: Patients participating in this study will have a diagnosis of 
FXS through molecular documentation of Fragile X Mental Retardation 1 (FMR1) full mutation.  No 
more than 25% of the patients screened will be females.  Siblings may be screened and enrolled based 
upon Investigator discretion and discussion with the Medical Monitor.  Patients must qualify based on 
meeting all of the inclusion and none of the exclusion criteria to be eligible to enroll. 
Inclusion Criteria: 
1. Male or female children and adolescents aged 3 to <18 years, at the time of Screening.  
2. Judged by the Investigator to be in generally good health at Screening based upon the results of a 
medical history, physical examination, 12-lead ECG, and clinical laboratory test results.  
Laboratory results outside of the reference range must be documented as not clinically significant 
by both the Investigator and Sponsor. 
3. Patients must have a diagnosis of FXS through molecular documentation of FMR1 full mutation. 
4. Patients with an ABC-CFXS 
 score of 
 at Screening OR patients with an 
ABC-CFXS 
 score of 
 at Screening with an ABC-CFXS 
 
score 
. 
5. Patients have a Clinical Global Impressions-Severity (CGI-S) score of at least ‘3’ at Screening.  
6. Patients with a history of seizure disorders must currently be receiving treatment with a stable 
regimen of one or two AEDs, or must be seizure-free for one year if not currently receiving AEDs. 
7. Patients taking AEDs should be on a stable regimen for the four weeks preceding study Screening 
and taking no more than two.  
8. Patients who are taking psychotropic medication(s) should be on a stable regimen of no more than 
two such medications for at least four weeks preceding study Screening and must maintain that 
regimen throughout the study.  Psychotropic medications include (but are not limited 
to) antipsychotics, antidepressants, anxiolytics, and attention-deficit / hyperactivity disorder 
(ADHD) medications. 
9. If patients are receiving non-pharmacological behavioral and/or dietary interventions, they must be 
stable and have been doing so for three months prior to Screening. 
10. Patients have a body mass index between 12–30 kg / m2 (inclusive). 
11. Females of childbearing potential must have a negative serum pregnancy test at the Screening Visit 
and a negative serum or urine pregnancy test at all designated visits. 
12. Patients and parents/caregivers agree to abide by all study restrictions and comply with all study 
procedures. 
13. Patients and parents/caregivers must be adequately informed of the nature and risks of the study 
and give written informed consent (and assent if applicable) prior to Screening. 
14. Parents/caregiver(s) must provide written consent to assist in trial drug administration. 
15. In the Investigator’s opinion, patients and parents/caregivers are reliable and willing and able to 
comply with all protocol requirements and procedures. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
10 
Exclusion Criteria: 
Any of the following is considered criterion for exclusion: 
1. Females who are pregnant, nursing, or planning a pregnancy; females of childbearing potential 
and male patients with a partner of childbearing potential who are unwilling or unable to use an 
acceptable method of contraception as outlined below for the duration of therapy and for three 
months after the last dose of study medication. 
Standard acceptable methods of contraception include abstinence or the use of a highly 
effective method of contraception, including hormonal contraception, diaphragm, cervical cap, 
vaginal sponge, condom, spermicide, vasectomy, or intrauterine device. 
2. History of significant allergic condition, significant drug-related hypersensitivity, or allergic 
reaction to any compound or chemical class related to ZYN002 or its excipients. 
3. Exposure to any investigational drug or device ≤ 30 days prior to Screening or at any time 
during the study. 
4. Patient has alanine aminotransferase (ALT), aspartate aminotransferase (AST), or total bilirubin 
levels ≥ 2 times the upper limit of normal (ULN) or has alkaline phosphatase levels ≥ 3 times 
the ULN as determined from Screening safety laboratories. 
5. Use of cannabis or any THC or CBD-containing product (aside from ZYN002) within three 
months of Screening Visit or during the study.  
6. Patient has a positive drug screen, including ethanol, cocaine, THC, barbiturates, amphetamines 
(unless prescribed), benzodiazepines (except midazolam or comparable administered for blood 
draws and ECG collection), and opiates.  
7. Patient is using the following AEDs: clobazam, phenobarbital, ethosuximide, felbamate, or 
vigabatrin.  
8. Patient is using any strong inhibitor/inducer of CYP3A4 or sensitive substrate for CYP3A4 
including but not limited to the following medications: midazolam (except single doses 
administered for the purposes of obtaining blood samples and ECG’s), oral ketoconazole, 
fluconazole, nefazadone, rifampin, alfentanil, alfuzosin, amiodarone, cyclosporine, dasatinib, 
docetaxol, eplerenone, ergotamine, everolimus, fentanyl, halofantrine, irinotecan, lapatinib, 
levomethadyl, lumefantrine, nilotinib, pimozide, quinidine, ranolazine, sirolimus, tacrolimus, 
temsirolimus, toremifene, tretinioin, vincristine, vinorelbine, and St. John’s Wort.  
9. Patients may not be taking minocycline for 30 days prior to Screening or throughout the study. 
10. Patients may not be taking any benzodiazepines (except single doses administered for the 
purposes of obtaining blood samples and ECG’s) at screening or throughout the study. 
11. Patient has an advanced, severe, or unstable disease that may interfere with the study outcome 
evaluations. 
12. Patient is expected to initiate or change pharmacologic or non-pharmacologic interventions 
during the course of the study. 
13. Patient has an acute or progressive neurological disease, psychosis, schizophrenia, or any 
psychiatric disorder or severe mental abnormalities (other than Fragile X Syndrome) that are 
likely to require changes in drug therapy or interfere with the objectives of the study or the 
ability to adhere to protocol requirements. 
14. Patient has a positive result for the presence of HBsAg, HCV, or HIV antibodies. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
11 
15. Patient has known history of cardiovascular disease, advanced arteriosclerosis, structural cardiac 
abnormality, cardiomyopathy, serious heart rhythm abnormalities, coronary artery disease, 
cardiac conduction problems, exercise-related cardiac events including syncope and pre-
syncope, risk factors for Torsades de pointes (TdP) (e.g., heart failure, hypokalemia, family 
history of Long QT Syndrome), or other serious cardiac problems. 
16. Any clinically significant condition or abnormal findings at the Screening Visit that would, in 
the opinion of the Investigator, preclude study participation or interfere with the evaluation of 
the study medication. 
17. Any skin disease or condition, including eczema, psoriasis, melanoma, acne, contact dermatitis, 
scarring, imperfections, lesions, tattoos, or discoloration, that may affect treatment application, 
application site assessments, or absorption of the trial drug. 
18. History of treatment for, or evidence of, drug abuse within the past year. 
19. Previous participation in a ZYN002 study. 
20. Patient responds “yes” to Question 4 or 5 on the C-SSRS (Children) during Screening or at any 
time on study. 
Investigational Product, Dosage, and Mode of Administration: ZYN002 gel, or Placebo gel, 
topical.  
Placebo Lead-In Period: 
During the single-blind Placebo Lead-In period patients < 35 kg will receive placebo, applied Q12H 
(± 2 hours).  Each application will consist of one sachet of placebo containing 2.98 g of gel (two 
sachets in total per day).  Patients > 35 kg will receive placebo, applied Q12H (+2 hours).  Each 
application will consist of two sachets of placebo containing 2.98 g of gel in each sachet (four sachets 
in total per day). 
Treatment Period: 
At Visit 3 patients will be randomized to either: 
Treatment A (Trial Drug)  
Patients < 35 kg will receive 125 mg CBD applied Q12H (± 2 hours); total daily dose of 250 mg CBD. 
Each application will consist of one sachet of ZYN002 CBD 4.2% concentration, containing 2.98 g of 
gel.  This dosing will continue for the duration of the study. 
Patients >35 kg will receive 250 mg CBD applied Q12H (+2 hours); total daily dose of 500 mg CBD.  
Each application will consist of two sachets of ZYN002 CBD 4.2% concentration, each sachet 
containing 2.98 g of gel.  This dosing will continue for the duration of the study. 
OR 
Treatment B (Placebo) 
Patients < 35 kg will receive placebo, applied Q12H (+2 hours).  Each application Q12H (± 2 hours), 
will consist of one sachet of placebo, containing 2.98 g of gel.  This dosing will continue for the 
duration of the study. 
Patients > 35 kg will receive placebo, applied Q12H (+2 hours).  Each application Q12H (+2 hours) 
will consist of two sachets of placebo, each sachet containing 2.98 g of gel.  This dosing will continue 
for the duration of the study. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
12 
Duration of Treatment: 
Parents/caregivers will apply trial drug twice daily for the 
 single-blind placebo lead-in 
period, followed by 12 weeks of randomized treatment twice daily.  Following Week 12, patients on 
AEDs may have an additional one or two weeks of blinded treatment to taper off trial drug. 
Criteria for Evaluation: 
Efficacy: 
Efficacy assessments are as indicated in Table 3 Schedule of Assessments.  
The primary endpoint is the change from Baseline to Week 12 in the ABC-CFXS Social Avoidance 
subscale score.  
Key Secondary Endpoints Include: 
a. Change from Baseline to Week 12 in ABC-CFXS Irritability subscale score. 
b. Change from Baseline to Week 12 in ABC-CFXS Socially Unresponsive/Lethargic subscale 
score.  
c. CGI-I at Week 12/ET. 
Secondary Endpoints Include:  Secondary Endpoints include: 
a. Percent of patients who have ≥ 25% improvement from Baseline in ABC-CFXS Social 
Avoidance subscale score at Week 12/ET. 
b. Percent of patients who have ≥ 25% improvement from Baseline in ABC-CFXS Irritability 
subscale score at Week 12/ET. 
c. Change from Baseline in ABC-CFXS Social Avoidance, Irritability, and Socially 
Unresponsive/Lethargic subscale scores at Weeks 4 and 8. 
d. Change from Baseline in ABC-CFXS, Stereotypy, Inappropriate Speech, and Hyperactivity 
subscale scores at Weeks 4, 8, and 12.  
e. Change from Baseline to Weeks 4, 8, and 12 in ADAMS total score and subscale scores 
(Manic/Hyperactive Behavior, Depressed Mood, Social Avoidance, General Anxiety and 
Compulsive Behavior).  
f. CGI-I at Weeks 4 and 8. 
g. Percent of patients indicating improvement on the CGI-I (dichotomized) scale at Weeks 4, 
8, and 12/ET. 
h. Response rate for patients having both ≥ 25% improvement from Baseline in ABC-CFXS 
Social Avoidance subscale score AND improved on CGI-I scale at Weeks 4, 8, and 12/ET. 
i. 
Response rate for patients having both ≥ 25% improvement from Baseline in ABC-CFXS 
Irritability subscale score AND improved on CGI-I scale at Weeks 4, 8, and 12/ET. 
j. 
Change from Baseline in CGI-S at Weeks 4, 8, and 12/ET. 
k. Change from Baseline in Family Impact PedsQLTM at Week 12/ET. 
The efficacy population is defined as all patients who have taken at least one dose of double-blind 
study medication and have at least one post-randomization ABC-CFXS assessment. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
13 
Safety Analyses:  
Safety assessments will include collection of AEs including seizure assessment if applicable, physical 
and neurological examination, Tanner Stage assessment, 12-lead ECG, clinical laboratory assessments 
(hematology, chemistry, and urinalysis), vital signs, C-SSRS, MWC, PWC-20, and findings from the 
skin check examinations following treatment.  All patients who receive at least one dose of 
randomized trial drug will be included in the safety analysis.    
Plasma Concentrations of CBD, THC and AEDs: 
Plasma concentrations for CBD, THC and concurrent AEDs will be summarized by randomized 
treatment group (active or placebo) and by Screening and treatment time points.  Plasma may also be 
analyzed for CBD metabolite concentrations.  
 
 
 
Statistical Methods: 
The sample size was estimated for the primary efficacy endpoint using Power Analysis and Sample 
Size Software (PASS 15) (NCSS, 2017).  Sample size requirements were based on a previous Phase 1/2 
trial, ZYN2-CL-009, in patients with FXS and on published results in the literature with other studied 
treatments in this indication.  A sample size of 102 patients per treatment group (204 patients total) is 
required for the detection of a 1.3-point difference (assuming a standard deviation of 2.8) in the ABC-
CFXS Social Avoidance subscale score between treatments (active versus placebo) using a 2-sided test 
at the 5% significance level with 90% power. 
Descriptive statistics (mean, median, standard deviation, minimum, and maximum) for continuous data 
and number (n) and percentage (%) for categorical data will be presented for all efficacy and safety 
parameters.  
All efficacy assessments will be summarized by randomized treatment group.  
Unless otherwise stated, mixed effects models for repeated measures (MMRM) will contain fixed 
effects for treatment, visit, study site, region and treatment-by-visit interaction as factors as well as the 
continuous fixed covariate of baseline value for the dependent variable of interest.   
Analysis of covariance (ANCOVA) models will contain fixed effects for treatment and study site and 
region with baseline value for the dependent variable of interest as a covariate. 
For MMRM/ANCOVA, descriptive statistics will be presented by visit, which will include least-
squares mean, least-squares standard errors, and p-values for between treatment group tests.  
The statistical comparison of active versus placebo for the primary endpoint of change from baseline in 
ABC-CFXS Social Avoidance scores will be based on MMRM.   The same model used for the primary 
endpoint analysis will also be used for the key secondary endpoints of change from Baseline in ABC-
CFXS Irritability, and Socially Unresponsive/Lethargic subscale scores.  The statistical comparison of 
active versus placebo for the key secondary endpoint of CGI-I will be based on Wilcoxon rank sum 
test.   
Response rate for the secondary endpoint defined as the number of patients who have ≥ 25% 
improvement from baseline in ABC-CFXS Social Avoidance subscale score will be compared using a 
chi-square test and binomial confidence intervals.   Response rate for the secondary endpoint defined 
as the number of patients who have ≥ 25% improvement from baseline in ABC-CFXS Irritability 
subscale score will be compared using a chi-square test and binomial confidence intervals.  The 
statistical comparison for all secondary endpoints for changes from baseline in ABC-CFXS and 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
14 
ADAMS subscale scores will be based on MMRM.  The statistical comparison of active versus 
placebo for the CGI-I at weeks 4 and 8 will be based on Wilcoxon rank sum test.   
The response rates for the percent of patients improved using CGI-I, and for patients having both ≥ 
25% improvement from Baseline in ABC- CFXS Social Avoidance subscale scores AND improved on 
CGI-I scale will be compared using the chi-square test and binomial confidence intervals.   The 
response rates for the percent of patients improved using CGI-I, and for patients having both ≥ 25% 
improvement from Baseline in ABC- CFXS Irritability subscale scores AND improved on CGI-I scale 
will be compared using the chi-square test and binomial confidence intervals.   
The statistical comparison for active versus placebo for the Family Impact module of the Pediatric 
Quality of Life (PedsQLTM) will be based on ANCOVA.  The statistical comparison of active versus 
placebo for the CGI-S will be based on Cochran-Mantel-Haenszel test, adjusting for the baseline score 
as the covariate. 
Results of exploratory analysis of the Caregiver Global Impression of Severity, the Caregiver Global 
Impression of Change, and the Qualitative Caregiver reported Behavioral Survey will be reported 
using descriptive statistics. 
AEs will be tabulated by the actual treatment dose of trial drug received at the time of initiation of the 
adverse event and classified by system organ class and preferred term using the Medical Dictionary for 
Regulatory Activities (MedDRA).  Additionally, AEs will be tabulated overall (total number of AEs 
and total number of patients with AEs).  Data from the MADDERS® will be summarized and 
presented separately from other AEs.   
Symptoms of withdrawal will also be assessed by comparing change over time in the total score on the 
MWC and total score on the PWC-20 from Baseline to Week 12, weekly during the taper period, and 
weekly for four weeks after the patient stops study drug. 
Vital signs assessments (actual and change from Baseline) taken at Baseline, Weeks 4, 8, and 12 will 
be summarized using descriptive statistics and presented by actual treatment group.  
Safety laboratory and urinalysis assessments (actual and change from Baseline) will be summarized by 
actual treatment group. 
ECGs (actual and change from Baseline) will be summarized by actual treatment group.  ECG results 
including any clinically significant findings will be summarized at each study visit. 
Application site check will be summarized using counts and percentages at each respective application 
site check score (‘0’, ‘1’, ‘2’, ‘3’, or ‘4’) by actual treatment group.  

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
15 
3. 
TABLE OF CONTENTS, LIST OF TABLES, AND LIST OF 
FIGURES  
TABLE OF CONTENT 
1. 
INVESTIGATOR’S AGREEMENT ............................................................................2 
2. 
SYNOPSIS ...................................................................................................................4 
3. 
TABLE OF CONTENTS, LIST OF TABLES, AND LIST OF FIGURES ...............15 
4. 
LIST OF ABBREVIATIONS AND DEFINITIONS OF TERMS.............................20 
5. 
INTRODUCTION ......................................................................................................25 
5.1. 
Background .................................................................................................................25 
5.2. 
Non-clinical Summary ................................................................................................27 
5.2.1. 
Clinical Pharmacokinetics and Drug-Drug Interaction (DDI) Potential ....................30 
5.3. 
Clinical Summary .......................................................................................................30 
5.3.1. 
Literature Review .......................................................................................................30 
5.3.2. 
ZYN002 Phase 1 studies .............................................................................................32 
5.3.3. 
ZYN002 Phase 2A Studies .........................................................................................33 
5.3.4. 
Ongoing ZYN002 Clinical Studies .............................................................................34 
6. 
TRIAL OBJECTIVES AND PURPOSE ....................................................................36 
6.1. 
Primary Objective .......................................................................................................36 
6.2. 
Secondary Objectives .................................................................................................36 
7. 
INVESTIGATIONAL PLAN .....................................................................................37 
7.1. 
Overall Study Design ..................................................................................................37 
7.2. 
Number of Patients .....................................................................................................39 
7.3. 
Dose Rationale ............................................................................................................39 
7.4. 
Dose Schedule ............................................................................................................39 
7.5. 
Criteria for Study Withdrawal ....................................................................................40 
7.6. 
Study Assessments......................................................................................................40 
7.6.1. 
Overview of Study Assessments ................................................................................40 
7.6.2. 
Informed Consent .......................................................................................................45 
7.6.3. 
Fragile X Diagnosis ....................................................................................................45 
7.6.4. 
Optional IQ Test Results ............................................................................................45 
7.6.5. 
Demographics .............................................................................................................45 
7.6.6. 
Medical History ..........................................................................................................45 
7.6.7. 
Vital Signs ..................................................................................................................45 
7.6.8. 
Concomitant Medication Review ...............................................................................45 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
16 
7.6.9. 
Adverse Event Review ...............................................................................................45 
7.6.10. 
Complete and Targeted Physical and Neurological Examinations .............................45 
7.6.11. 
Tanner Stage Assessment ...........................................................................................46 
7.6.12. 
Electrocardiogram .......................................................................................................46 
7.6.13. 
Clinical Laboratory Testing ........................................................................................46 
7.6.13.1. 
Screening, Visit 4, Visit 6/ET and EOS Laboratory Assessments .............................47 
7.6.14. 
Serum and Urine Pregnancy Tests ..............................................................................48 
7.6.15. 
Skin Assessment Examination ....................................................................................48 
7.6.16. 
Skin Irritation Check Examination .............................................................................48 
7.6.17. 
Blood Samples for AEDs, CBD and THC Levels ......................................................49 
7.6.18. 
Blood Samples for CGG Analysis ..............................................................................49 
7.6.19. 
Assessments of FXS Symptomatology .......................................................................50 
7.6.19.1. 
Aberrant Behavior Checklist-Community FXS Specific (ABC-CFXS) .......................50 
7.6.19.2. 
Autism Diagnostic Observation Schedule® (ADOS®-2) ............................................50 
7.6.19.3. 
Anxiety, Depression, and Mood Scale (ADAMS) .....................................................51 
7.6.19.4. 
Clinical Global Impressions Scale-Severity and Improvement ..................................51 
7.6.19.5. 
Caregiver Global Impression of Severity and Change Scales ....................................51 
7.6.19.6. 
Family Impact PedsQLTM (Pediatric Quality of Life Inventory) ................................51 
7.6.19.7. 
Vineland Adaptive Behavior Scales™, Third Edition (VABS-3) ..............................52 
7.6.20. 
Columbia Suicide Severity Rating Scale (C-SSRS) (Children’s Version) .................52 
7.6.21. 
Marijuana Withdrawal Checklist - Short form (MWC, Behavior Checklist) .............52 
7.6.22. 
Penn Physician Withdrawal Checklist (PWC-20) ......................................................52 
7.7. 
End of Study (EOS) Visit and Early Termination (ET) Visit .....................................53 
8. 
SELECTION AND WITHDRAWAL OF PATIENTS ..............................................54 
8.1. 
Patient Inclusion Criteria ............................................................................................54 
8.2. 
Patient Exclusion Criteria ...........................................................................................55 
8.3. 
Randomization Criteria ...............................................................................................56 
8.3.1. 
Sibling Options ...........................................................................................................56 
8.4. 
Patient Withdrawal Criteria ........................................................................................57 
9. 
TREATMENT OF PATIENTS ..................................................................................58 
9.1. 
Description of Trial Drug ...........................................................................................58 
9.2. 
Concomitant Medications ...........................................................................................58 
9.2.1. 
Concomitant Medications Allowed ............................................................................59 
9.2.1.1. 
Psychotropic Medications ...........................................................................................59 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
17 
9.2.2. 
Concomitant Medications Not Allowed .....................................................................60 
9.3. 
Treatment Compliance ................................................................................................60 
10. 
TRIAL DRUG MATERIALS AND MANAGEMENT .............................................61 
10.1. 
Trial Drug ...................................................................................................................61 
10.2. 
Trial Drug Packaging and Labeling ............................................................................61 
10.3. 
Trial Drug Storage ......................................................................................................61 
10.4. 
Trial Drug Preparation ................................................................................................61 
10.5. 
Administration ............................................................................................................61 
10.6. 
Trial Drug Accountability ...........................................................................................62 
10.7. 
Trial Drug Handling and Disposal ..............................................................................62 
11. 
ASSESSMENT OF EFFICACY ................................................................................63 
11.1. 
Primary Endpoint ........................................................................................................63 
11.2. 
Key Secondary Endpoints ...........................................................................................63 
11.3. 
Secondary Endpoints ..................................................................................................63 
11.4. 
Method of Treatment Assignment or Randomization ................................................64 
11.5. 
Breaking the Blind ......................................................................................................64 
12. 
ASSESSMENT OF SAFETY .....................................................................................65 
12.1. 
Safety Parameters .......................................................................................................65 
12.2. 
Adverse and Serious Adverse Events .........................................................................65 
12.2.1. 
Definition of Adverse Events .....................................................................................65 
12.2.1.1. 
Adverse Event (AE) ....................................................................................................65 
12.2.1.2. 
Serious Adverse Event (SAE) ....................................................................................67 
12.2.1.3. 
Other Adverse Event (OAE) .......................................................................................67 
12.3. 
Recording Adverse Events .........................................................................................67 
12.4. 
Reporting Adverse Events ..........................................................................................68 
13. 
ASSESSMENT OF PHARMACOKINETICS ...........................................................70 
13.1.1. 
Blood Levels of AED, CBD and THC .......................................................................70 
13.1.1.1. 
Blood Sample Collection ............................................................................................70 
13.1.1.2. 
Sample Analysis .........................................................................................................70 
14. 
STATISTICS ..............................................................................................................71 
14.1. 
Sample Size Determination ........................................................................................71 
14.2. 
Analysis Populations ..................................................................................................71 
14.3. 
Efficacy Analysis ........................................................................................................71 
14.3.1. 
Primary Efficacy Analyses .........................................................................................71 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
18 
14.3.2. 
Key Secondary Efficacy Analyses ..............................................................................72 
14.3.3. 
Secondary Efficacy Analyses .....................................................................................72 
14.3.4. 
Exploratory Analysis ..................................................................................................73 
14.4. 
Safety Analysis ...........................................................................................................73 
14.5. 
Pharmacokinetic Analysis ..........................................................................................73 
14.6. 
Interim Analysis ..........................................................................................................74 
14.7. 
Adjustments for Multiplicity ......................................................................................74 
15. 
DIRECT ACCESS TO SOURCE DATA/DOCUMENTS.........................................75 
15.1. 
Study Monitoring ........................................................................................................75 
15.2. 
Audits and Inspections ................................................................................................75 
15.3. 
Institutional Review Board (IRB)/Independent Ethics Committee (IEC) ..................76 
16. 
QUALITY CONTROL AND QUALITY ASSURANCE .........................................77 
17. 
ETHICS ......................................................................................................................78 
17.1. 
Independent Ethics Committee or Institutional Review Board ..................................78 
17.2. 
Ethical Conduct of the Study ......................................................................................78 
17.3. 
Patient Information and Informed Consent ................................................................78 
18. 
DATA HANDLING AND RECORDKEEPING .......................................................80 
18.1. 
Inspection of Records .................................................................................................80 
18.2. 
Retention of Records ..................................................................................................80 
19. 
PROTOCOL MODIFICATIONS IMPLEMENTED DURING THE 
COVID-19 PANDEMIC ............................................................................................81 
19.1. 
Changes to Allow for Remote Patient Visits ..............................................................81 
19.2. 
Changes to Allow for Investigational Product (IP) Shipment from 
Investigative Site to Parent/Legal Guardian ...............................................................81 
19.3. 
Changes to Allow for Courier Return of Investigational Product from 
Parent/Legal Guardian to an Approved Depot ...........................................................82 
19.4. 
Changes to Allow Patients to Attend Local Laboratory Facilities to Obtain 
Blood Collection for Safety Laboratory Analyses ......................................................82 
19.5. 
Shifting of Some Safety Assessments to the Next Onsite Visit .................................82 
20. 
PUBLICATION POLICY ..........................................................................................84 
21. 
LIST OF REFERENCES ............................................................................................85 
22. 
APPENDICES ............................................................................................................91 
22.1. 
Tanner Stage Assessment ...........................................................................................91 
22.2. 
Daily Skin Check Diary ..............................................................................................92 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
19 
LIST OF TABLES 
Table 1:  Emergency Contact Information.......................................................................................3 
Table 2:  Abbreviations and Specialist Terms ...............................................................................20 
Table 3:  Schedule of Assessments ................................................................................................42 
Table 4:  Laboratory Assessments .................................................................................................47 
Table 5:  Skin Irritation Check Scale .............................................................................................49 
 
 
 
LIST OF FIGURES 
Figure 1:  Mechanism of Impaired Endocannabinoid Signaling in Fragile X ...............................26 
Figure 2:  Study Design .................................................................................................................38 
 
 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
20 
4. 
LIST OF ABBREVIATIONS AND DEFINITIONS OF TERMS 
The following abbreviations and specialist terms are used in this study protocol. 
 
Table 2:  Abbreviations and Specialist Terms  
Abbreviation or 
Specialist Term 
Explanation 
2-AG 
2-arachidonoylglycerol 
5-HT1A 
Serotonin 
AE 
Adverse event 
ABC-CFXS  
Aberrant Behavior Checklist-FXS factor analysis 
ADAMS 
Anxiety Depression and Mood Scale 
ADHD 
Attention-deficit / hyperactivity disorder 
ADOS-2 
Autism Diagnostic Observation Schedule®-2 
AEA 
Anandamide 
AED 
Anti-epileptic drug 
ALT 
Alanine transaminase  
ANCOVA 
Analysis of covariance 
API 
Active Pharmaceutical Ingredient 
AST 
Aspartate transaminase 
AUC 
Area under the curve 
BID 
Twice Daily 
BUN 
Blood urea nitrogen 
°C 
Degrees Celsius 
CB1 
Cannabinoid receptor type 1 
CB2 
Cannabinoid receptor type 2 
CBD 
Cannabidiol 
CFR 
Code of Federal Regulations  
CGG 
Cytosine Guanine Guanine 
CGI-I 
Clinical Global Impression-Improvement 
CGI-S 
Clinical Global Impression-Severity 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
21 
Table 2:  Abbreviations and Specialist Terms (Continued) 
Abbreviation or 
Specialist Term 
Explanation 
CI 
Confidence Interval 
Cmax  
Maximum observed concentration 
CMC 
Chemistry, Manufacturing, and Control 
CMH 
Cochran-Mantel-Haenszel 
CNS 
Central nervous system 
CONNECT-FX 
Clinical study Of caNNabidiol in childrEn and adolesCenTs with 
Fragile X 
CRA 
Clinical Research Associate 
CRF 
Case report form 
C-SSRS 
Columbia-Suicide Severity Rating Scale 
CTRA 
Clinical Trial Research Agreement 
CYP 
Cytochrome P enzyme 
DDI 
Drug-drug interaction 
DEA 
Drug Enforcement Agency 
DGL 
Diacylglycerol lipase 
DNA 
Deoxyribonucleic acid 
ECG 
Electrocardiogram 
e.g. 
Exampli Gratia – ‘for example’ 
EOS 
End of Study 
ET 
Early Termination 
et al. 
Et alia – ‘and others’ 
°F 
Degrees Fahrenheit 
FAAH 
Fatty acid amide hydrolase 
FABP 
Fatty acid binding proteins 
FAS 
Full Analysis Set 
FDA 
Food and Drug Administration 
FMR1 
Fragile X Mental Retardation 1 
FMRP 
Fragile X Mental Retardation protein 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
22 
Table 2:  Abbreviations and Specialist Terms (Continued) 
Abbreviation or 
Specialist Term 
Explanation 
FXS 
Fragile X Syndrome 
g 
Gram 
GCP 
Good clinical practice 
GLP 
Good laboratory practice 
HBsAg 
Hepatitis B Surface antigen 
HCV 
Hepatitis C virus 
HDL 
High density lipoprotein  
HIPAA 
Health Insurance Portability and Accountability Act  
HIV 
Human immunodeficiency virus 
HPLC 
High-performance liquid chromatography 
HRQOL 
Health-related quality of life 
ICF 
Informed consent form 
ICH 
International Conference on Harmonization 
i.e. 
id est – ‘that is’ 
IEC 
Independent Ethics Committee 
IP 
Investigational Product 
IQ 
Intelligence Quotient 
IRB 
Institutional Review Board 
IRT 
Interactive Response System 
IV 
Intravenous 
kg 
Kilogram 
KO 
Knock-out 
LDL 
Low density lipoprotein 
L 
Liter 
LS 
Least-squares 
LTD 
Long-term depression 
 
 
 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
23 
Table 2:  Abbreviations and Specialist Terms (Continued) 
Abbreviation or 
Specialist Term 
Explanation 
MADDERS® 
The Misuse Abuse and Diversion Drug Event Reporting System 
MedDRA 
Medical Dictionary for Regulatory Activities 
mg 
Milligram 
mL 
Milliliter 
MWC 
Marijuana Withdrawal Checklist 
MMRM 
Mixed effects models for repeated measures 
MRI 
Magnetic resonance imaging 
MS/MS 
Tandem mass spectrometry 
N 
Number  
ng 
Nanogram 
nm 
Nanometers 
NOAEL 
No-observed-adverse-effect-levels 
NONMEM 
Nonlinear mixed effects model 
OA 
Osteoarthritis 
OAE 
Other significant adverse event 
OLE 
Open Label Extension 
OR 
Odds Ratio 
OTC 
Over-the-counter 
PASS 
Power Analysis and Sample Size 
PedsQL™ 
Pediatric Quality of Life Inventory 
PI  
Principal Investigator 
The investigator who leads the study conduct at an individual study 
center.  Every study center has a principal investigator. 
PK 
Pharmacokinetic 
PND 
Post-natal day 
PWC-20 
Penn Physician Withdrawal Checklist 
Q12H 
Every 12 hours 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
24 
Table 2:  Abbreviations and Specialist Terms (Continued) 
Abbreviation or 
Specialist Term 
Explanation 
RBC 
Red blood cell 
RNA 
Ribonucleic acid 
SAE 
Serious adverse event 
SAP 
Statistical Analysis Plan 
SC 
Subcutaneous 
SGOT 
Serum glutamic oxaloacetic transaminase 
SGPT 
Serum glutamic pyruvic transaminase 
SSRIs 
Selective serotonin reuptake inhibitors 
T 
Testosterone  
t1/2 
Half-life 
TdP 
Torsades de pointes 
TEAE 
Treatment emergent adverse event 
THC 
Δ9-tetrahydrocannabinol 
UDS 
Urine Drug Screen 
ULN 
Upper limit of normal 
UK 
United Kingdom 
UV 
Ultraviolet 
VABS-3 
Vineland Adaptive Behavior Scales™, Third Edition 
VAS 
Visual Analog Scale 
VR1 
Vanilloid type-1 
vs 
Versus 
WBC 
White blood cell 
WDS 
Withdrawal Discomfort Score 
 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
25 
5. 
INTRODUCTION 
5.1. 
Background 
The Drug Product ZYN002 is a transdermal CBD gel. CBD is the primary non-euphoric 
cannabinoid contained in the Cannabis sativa L. plant.  The CBD contained within ZYN002 is a 
pharmaceutically produced Active Pharmaceutical Ingredient (API) that is chemically identical 
to the CBD present in Cannabis.   
Fragile X Syndrome (FXS) is a rare genetic condition caused by a mutation in the FMR1 gene 
(Haldeman-Englert 2013) located on the X chromosome.  Mutations in the FMR1 gene, which 
silence the expression of the Fragile X mental retardation protein (FMRP), are characteristically 
found in patients with FXS.  FMRP, a ribonucleic acid (RNA) binding protein, is important for 
normal synaptic function, synaptic plasticity, and the development of neuronal connections over 
time during brain maturation.  The absence of FMRP in neurons accounts for many of the 
neuropsychiatric symptoms of Fragile X.  Both males and females are at risk of developing 
symptoms linked to FXS.  However, since the responsible gene is on the X chromosome, and 
only one changed (mutated) copy of the responsible gene is enough to cause the symptoms, FXS 
is inherited in an X-linked dominant pattern.  In females (who have two X chromosomes), a 
mutation in one of the two genes can be sufficient to cause the disorder.  In males (who have 
only one X chromosome), a mutation in the only copy of the gene can cause the disorder.  While 
females are more likely to suffer from FXS, and are at increased risk of having a child with FXS, 
males typically exhibit more severe symptoms because of the X inactivation (Crawford et al. 
2001). 
FXS is characterized by many clinical symptoms, including anxiety (particularly social 
anxiety/avoidance), deficits in learning and cognition, sleep difficulties, seizures, social 
unresponsiveness and stereotypy (Lozano et al. 2015).  These symptoms are typically managed 
by medications including melatonin and clonidine for sleep, selective serotonin reuptake 
inhibitors (SSRIs) and benzodiazepines for anxiety, psychostimulants for hyperactivity and 
deficits in attention, and carbamazepine, valproic acid, and lamotrigine for seizures (Fragile X 
Clinical Research Consortium 2012).  Unfortunately, many of these medications are ineffective 
for a number of patients with FXS and lead to multiple deleterious consequences (e.g., agitation, 
diarrhea, dizziness, nausea, vomiting, hair loss, memory, and sleep problems), some of which 
coincide with symptoms already experienced by patients with FXS (Fragile X Clinical Research 
Consortium 2012; Lozano et al. 2015).  CBD is believed to have the potential for attenuating the 
loss of endogenous endocannabinoid signaling in FXS, allowing the FMRP protein deficiency 
inherent in FXS to be bypassed.  Abnormalities seen in FXS appear to be rooted in dysregulation 
of the endocannabinoid pathways in the central nervous system.  The endocannabinoid system 
consists of receptors in the brain and peripheral tissues that are involved in numerous 
physiological processes, as well as the endogenous cannabis-like ligands (endocannabinoids): 
anandamide (AEA) and 2-arachidonoylglycerol (2-AG).  The endocannabinoids bind to G-
protein-coupled receptors, Cannabinoid receptor type 1 (CB1) and Cannabinoid receptor type 2 
(CB2) (Mouslech and Valla 2009; Pacher et al. 2006) and modulate synaptic transmission 
throughout the central nervous system (Castillo et al. 2012; Ohno-Shosaku and Kano 2014).  CB1 
receptors are abundantly expressed in the brain, and they are present at lower concentrations in a 
variety of peripheral tissues and cells.  CB2 receptors are expressed primarily in the immune and 
hematopoietic systems, as well as in the brain, pancreas, and bone (Pacher et al. 2006). 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
26 
In patients with FXS, there is a reduction of endogenous stimulation of endocannabinoid 
receptors (see Figure 1).  CBD has the capacity to interact with an FXS-compromised 
endocannabinoid system.  Disruption of FMRP in FXS reduces the production of 2-AG, 
decreasing activation of CB1 receptors in the central nervous system (Jung et al. 2012).  CBD has 
been hypothesized to increase 2-AG availability (Elmes et al. 2015; Di Marzo and Maccarrone 
2008), potentially attenuating or reversing one of the biological mechanisms of abnormal cellular 
function in FXS (Jung et al. 2012).  Importantly, studies have shown that CB1 protein expression 
is unaffected in FMR1 Knock Out (KO) mice, suggesting that the downstream elements of 
endocannabinoid signaling can be engaged even in the absence of FMRP (Zhang and Alger 
2010). 
Figure 1:  Mechanism of Impaired Endocannabinoid Signaling in Fragile X 
 
Legend: Disruption of FMRP (red X) in FXS reduces the production of 2-AG (clear circles), decreasing activation 
of CB1 receptors in the central nervous system.  Loss of FMRP leads to a reduction of 2-AG production because the 
lack of FMRP expression disrupts diacylglycerol lipase (DGL) activity. (Jung et al. 2012) 
In addition to the role of 2-AG, recent work has begun to highlight the potential importance of 
AEA in impacting social impairment as well as deficits in learning and memory among those 
with FXS.  In an FMR1 KO mouse model of FXS, Qin and colleagues (2015) demonstrated that 
increased levels of AEA were associated with greater cognitive performance.  Similarly, Wei et 
al. (2016) utilized a mouse model of FXS to show that increasing AEA activity resulted in 
improvements in social impairment.  Much like its impact on 2-AG, CBD has been shown to 
increase levels of AEA (by binding to fatty acid binding proteins; FABP).  FABPs are 
intracellular proteins that transport AEA to the catabolic enzyme fatty acid amide hydrolase 
(FAAH), an enzyme that breaks down AEA (Figure 4 in Bisogno et al. 2001; Table 3 in Pertwee 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
27 
2008; Leweke et al. 2012; Elmes et al. 2015).  Binding to FABP is thought to result in increased 
AEA availability and greater CB1 activation. 
Beyond providing benefit to FXS patients via increases in 2-AG and AEA availability, CBD may 
also affect synaptic plasticity.  Mice lacking FMRP show alterations in synaptic plasticity, as 
evidenced by a reduction in long-term depression (LTD) of neuronal response properties in in 
vitro slice preparations (Zhang and Alger 2010).  Preclinical data suggest that restoration of LTD 
in FMR1 KO mice requires activation of endocannabinoid receptors (Figure 4A in Zhang and 
Alger 2010).  Therefore, it is hypothesized that CBD will increase synaptic plasticity in FXS, 
facilitating one of the basic cellular mechanisms thought to be associated with learning and 
improvements in cognition (Zhang and Alger 2010).  Finally, more recent work has begun to 
identify deficits in GABA receptor expression among those with FXS.  As FMRP has been 
shown in animal models to enhance expression of GABA receptors, the lack of FMRP among 
those with FXS has been associated with fewer GABA receptors (Lozano et al. 2015).  Indeed, 
non-clinical studies in FMR1 KO mice have consistently shown down-regulation of the GABA 
system (Hare et al. 2014).  As CBD acts as a positive allosteric modulator of GABA-A receptors 
(Bakas et al. 2017), CBD may act to enhance the binding affinity for GABA. 
The existing literature combines to demonstrate that CBD may positively impact individuals with 
FXS through many mechanisms, including the endocannabinoid and GABA systems.  While a 
number of drugs have been developed to target specific systems (e.g., GABA agonists), CBD 
may serve as a treatment that is likely to yield a multi-faceted benefit to individuals with FXS.  
CBD has not only been shown to be generally well tolerated relative to other treatments used in 
this population (Rohleder et al. 2016), but numerous studies have documented its benefits in 
terms of sleep quality (Carlini and Cunha. 1981; Chagas et al. 2014), anxiety (Bergamaschi et al. 
2011; Viveros et al. 2005), cognitive impairment (Bergamaschi et al. 2011), and seizures 
(Friedman and Devinsky 2015).  Anxiety symptoms are commonly associated with FXS.  Both 
non-clinical (e.g., Blessing et al. 2015) and human (e.g., Bergamaschi et al. 2011) data have 
demonstrated the efficacy of CBD in the alleviation of anxiety symptoms.  Reviews have 
highlighted anxiolytic effects of CBD in over 30 animal studies, using models for multiple types 
of anxiety (i.e., generalized anxiety models, stress-induced anxiety models, panic disorder 
models, and contextual fear conditioning models), as well as in a growing number of human 
studies, particularly in social anxiety.  Several studies have explored potential mechanisms of 
action and demonstrated that the anxiolytic effects of CBD are likely due in large part to its 
effects on 5-HT1A (serotonin). 
5.2. 
Non-clinical Summary 
To support the clinical development of ZYN002, Zynerba has relied upon: 
• Data from non-clinical safety studies conducted by Zynerba with ZYN002, ZYN002 
placebo gel, or CBD, the active ingredient in ZYN002,  
• Publicly available information about the toxicity/safety of CBD and the excipients in 
ZYN002, and 
• Clinical safety data generated by Zynerba with CBD and ZYN002. 
Published non-clinical studies with CBD indicate that it does not produce adverse effects on 
nervous system, respiratory, or gastrointestinal function, although it does exhibit beneficial 
activity in some nervous system disorders (e.g., anticonvulsant and anxiolytic activity) and 
gastrointestinal disorders (e.g., anti-inflammatory activity).  In a good laboratory practice (GLP)-

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
28 
compliant study conducted by Zynerba, CBD did not affect cardiovascular system function even 
at an estimated plasma concentration (4350 ng/mL) that is 
 greater than the expected 
plasma maximum observed concentration (Cmax) in patients over a 14-day period 
.  
Based on this information, human subjects using ZYN002 are unlikely to experience adverse 
effects on nervous system, cardiovascular, respiratory, or gastrointestinal function. 
ZYN002 was devoid of systemic toxicity in animals given topical doses applied to 10% of their 
body surface area daily for up to 26 weeks (rats) or twice a day for up to 13 weeks (minipigs).  
 
 
 
 
 
 
  
 
 
 
 
 
   
 
 
 
 
 
  
 
 
 
• 
 
 
 
 
 
 
• 
 
 
  
 
 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
29 
• 
 
 
 
 
 
• 
 
   
 
 
 
• 
 
 
 
• 
 
 
 
 
 
 
 
 
 
 
Repeat-dose toxicity studies in rats and minipigs with ZYN002 have revealed no effects on 
endocrine systems or reproductive organs in either species.  Published information suggests that 
CBD has the potential to affect reproductive function in animals, at least in males, possibly 
secondary to effects on sex hormone levels (Dalterio et al. 1984; Dalterio and deRooij, 1986).  
Published information also suggests that administration of CBD to pregnant mice has the 
potential to affect development of offspring, at least to males (Dalterio et al. 1984; Dalterio and 
deRooij 1986). 
CBD does not absorb UV or visible light over 290-700 nm (Hazekamp et al. 2005) and so 
ZYN002 does not present a potential phototoxicity hazard to human subjects.   
 
 
 
 
 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
30 
5.2.1. 
Clinical Pharmacokinetics and Drug-Drug Interaction (DDI) Potential 
Results from three completed Phase 1 studies, along with a population PK model, which includes 
results from ZYN2-CL-03, ZYN2-CL-04 and ZYN2-CL-05, have adequately described the PK 
of CBD following application of ZYN002 transdermal gel.  Pharmacokinetic conclusions for 
these studies are as follows: 
• 
 
 
 
• THC was not quantifiable in either plasma or urine. 
• There is adequate animal NOAEL: human exposure ratios for AUC and Cmax.  
Population pharmacokinetic analyses indicate: 
• 
 
 
• 
 
Because CYP3A4 and CYP2C19 are the major isoforms responsible for CBD metabolism, 
concomitant administration of drugs that inhibit these enzymes may result in higher exposure to 
CBD and drugs that induce these enzymes may result in lower exposure to CBD; therefore, 
strong inhibitors or inducers of CYP3A4 may increase or decrease the plasma concentrations of 
CBD and should be administered with caution. 
As a potential perpetrator, CBD would not cause a clinically significant induction of CYP 
isoenzymes.  However, CBD exhibited time-dependent inhibition of CYP2D6 and CYP1A2, 
which was reversible, and of CYP3A4, which was irreversible.  Sensitive CYP3A4 substrates 
with a narrow therapeutic index or risk for severe toxicity should be avoided until this risk has 
been assessed with ZYN002.  CBD did not inhibit human efflux and uptake transporters.  
5.3. 
Clinical Summary  
CBD studies completed with oral, inhaled, and intravenous (i.v.) formulations support a 
favorable tolerability and efficacy profile in several disease states, including topline data for 
ZYN002 in FXS.  These efficacy and tolerability data provide a rationale for development of the 
transdermal delivery of synthetic CBD which is not subject to gastric acid degradation and first 
pass metabolism in the liver, and may achieve consistent blood levels for the treatment of child 
and adolescent patients with FXS. 
5.3.1. 
Literature Review 
Oral CBD has been clinically studied in healthy subjects and patients with a variety of 
conditions.  The literature studies help to clarify the clinical potential of ZYN002 transdermal gel 
in the following areas: 
• Most assessments have used a 600 mg oral dose of CBD, but subjects in several trials have 
been treated with oral CBD doses of 1200 mg or more (Zuardi et al. 2010; Matsuyama and 
Fu 1981), and one study employed a 1500 mg dose (Zuardi et al. 2006a).  More recent 
epilepsy studies have titrated doses up to 25 – 50 mg/kg of oral doses in patients (age 1-30 
years) (Devinsky et al. 2016; Devinsky et al. 2017).  The mean CBD dose at 12 weeks was 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
31 
22.9 mg/kg corresponding to a 732 mg oral dose for an average 10-year old patient.  At the 
highest dose, Zynerba has studied a 780 mg daily dose at a CBD concentration of 4.2% 
(ZYN002 4.2%, 4.64 g twice a day). 
Data from a recent randomized, double-blind, placebo-controlled trial of cannabidiol as add-on 
therapy in 171 patients with Lennox Gastaut syndrome, aged 2-55 years (mean of 15 years) 
supported the efficacy seen in the open-label study (Thiele et al. 2016).  Patients received an oral 
solution of cannabidiol [Epidiolex®, GW Pharmaceuticals, London, UK; 100 mg per mL sesame 
oil-based solution] titrated to a dose of 20 mg/kg/day or placebo in two divided doses.  A median 
reduction in total seizures of 45% in the CBD group versus 15% in the placebo group reported.  
Drop seizures were reduced by a median of 49% in the CBD group compared to 20% in the 
placebo group.  The most common adverse events (>10% of cannabidiol-treated patients) were 
similar to those seen in the open-label study and included diarrhea, somnolence, decreased 
appetite, pyrexia, and vomiting. 
Data supportive of the open-label results in patients in Dravet syndrome has also been reported 
from a recent randomized, double-blind, placebo-controlled trial of 120 children and young 
adults (mean age of 9.8 years, range 2.3 to 18.4 years) (Devinsky et al. 2017).  Patients received 
an oral solution of cannabidiol [Epidiolex®, GW Pharmaceuticals, London, UK; 100 mg/mL 
sesame oil-based solution] titrated to a dose of 20 mg/kg/day or placebo in two divided doses.  
The primary endpoint was the change in convulsive-seizure frequency over a 14-week treatment 
period, as compared with a four-week baseline period.  The median frequency of convulsive 
seizures per month decreased from 12.4 to 5.9 with cannabidiol, as compared with a decrease 
from 14.9 to 14.1 with placebo (adjusted median difference between the cannabidiol group and 
the placebo group in change in seizure frequency, -22.8 percentage points; 95% confidence 
interval [CI], -41.1 to -5.4; p=0.01).  The percentage of patients who had at least a 50% reduction 
in convulsive-seizure frequency was 43% with cannabidiol and 27% with placebo (OR: 2.00; 
95% CI; 0.93 to 4.30; p=0.08).  The frequency of total seizures of all types was significantly 
reduced with cannabidiol (p=0.03), but there was no significant reduction in non-convulsive 
seizures.  Adverse events that occurred more frequently in the cannabidiol group than in the 
placebo group included diarrhea, vomiting, fatigue, pyrexia, somnolence, and abnormal results 
on liver function tests. 
Long-term exposure: — The 600 mg oral dose of CBD has been monitored in multiple long-
term treatment situations.  In at least seven studies, study periods of 3 months have been used 
(Martin-Santos et al. 2012; Bhattacharyya et al. 2012; Winton-Brown et al. 2011; Fusar-Poli et 
al. 2010; Bhattacharyya et al. 2010; Fusar-Poli et al. 2009, Borgwardt et al. 2008), and several 
patients have taken CBD for 4.5 months (Cunha et al. 1980).  The 25 mg/kg oral doses were 
studied for 3 months in an open-label expanded access trial (Devinsky et al. 2016). 
Psychoactive effects: — Studies of the potential psychoactive effects associated with CBD have 
not been widely reported until recently.  Previous reports suggest the absence of psychoactive 
effects whether CBD is administered intravenously (Perez-Reyes et al. 1973) or orally (Englund 
et al. 2013; Martin-Santos et al. 2012; Bhattacharyya et al. 2012; Bhattacharyya et al. 2010; 
Zuardi et al. 2009).  And pre-treatment with oral CBD 600 mg has been shown to inhibit the 
psychosis and cognitive impairment associated with intravenous (IV) THC 1.5 mg (Englund et 
al. 2013; Bhattacharyya et al. 2010).  A recent placebo-controlled study with Epidiolex showed 
high rates of somnolence (36%), diarrhea (31%), fatigue (20%), vomiting (15%), and lethargy 
(13%) (Devinsky et al. 2017).  These effects could be due to drug, underlying disease, potential 
conversion of oral CBD to THC or a combination of these factors.  Previous work has shown that 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
32 
in the presence of acidic reagents, CBD isomerizes to tetrahydrocannabinol (Gaoni and 
Mecoulam 1966).  In simulated gastric fluid, CBD converts to Δ9-tetrahydrocannabinol, 9-α-
hydroxy-hexahydrocannabinol and 8-hydroxy-iso-hexahydrocannabinol.  All have psychoactive 
activity (Merrick et al. 2016; Watanabe et al. 2007). 
Effects on vital signs or clinical lab tests: — CBD-treated subjects in clinical studies have 
shown no treatment-related effects on key vital sign indicators, including blood pressure and 
heart rate (Martin-Santos et al. 2012; Hallak et al. 2011; Fusar-Poli et al. 2009; Borgwardt et al. 
2008; Zuardi et al. 1993a; Consroe et al. 1991; Zuardi et al. 1982, and Perez-Reyes et al. 1973), 
as well as electrocardiography (Guy and Flint 2003; Carlini and Cunha 1981; Cunha et al. 1980).  
Guy and Flint (2003) performed cardiac monitoring continually from pre-dose to four hours 
post-dose along with pre- and post-dose ECG.  Heart rate, PR interval, QT interval and QRS 
width were evaluated pre- and post-dose.  Elevated transaminases (ALT or AST) without 
association of drug induced liver injury have been identified in epilepsy studies of oral CBD in 
children and young adults (Devinsky et al. 2016; Devinsky et al. 2017; Bebin et al. 2017; 
Devinsky et al. 2018).  Most cases were associated with the concomitant use of valproic acid and 
resolved with continued treatment or with decreasing the dose of valproic acid, CBD or 
discontinuation of CBD. 
No association with response inhibition: — Findings from functional magnetic resonance 
imaging (MRI) and behavioral studies show that CBD modulates function in regions not usually 
implicated in response inhibition.  In terms of clinical sequelae, these data help to explain why 
CBD does not impair motor or cognitive performance and has anxiolytic effects (Borgwardt et 
al. 2008). 
5.3.2. 
ZYN002 Phase 1 studies 
Three Phase 1 (ZYN2-CL-01, ZYN2-CL-02, and ZYN2-CL-08) studies investigating ZYN002 
(CBD) administered via a transdermal delivery system have been conducted in healthy subjects 
and patients with epilepsy.  In addition, one placebo study (ZYN2-CL-06) was conducted to 
evaluate the skin tolerability of the excipients in the ZYN002 formulation. 
These efficacy and tolerability data provide a rationale for development of a transdermal delivery 
of synthetic CBD which is not subject to first pass metabolism and may achieve consistent blood 
levels for the treatment of patients with FXS and epilepsy. 
A summary of the safety results from the Zynerba studies further supports the development of 
ZYN002.  Four Phase 1 safety and tolerability study results as follows: 
• ZYN2-CL-01, a single rising-dose study in healthy subjects (n=32) and patients with 
epilepsy (n=10) receiving ZYN002 (50, 100, 125, and 250 mg) or placebo showed that 
ZYN002 was safe and well tolerated at all doses.  The incidence of treatment-emergent 
adverse events (TEAEs) associated with ZYN002 was similar to placebo in healthy 
volunteers.  There were no serious adverse events (SAEs), no clinically significant changes 
in ECGs, vital signs or clinical laboratory results.  ZYN002 had good skin tolerability, and 
there was no post-dosing erythema at 24, 48, 72, and 96 hours. 
• ZYN2-CL-02, a seven-day repeat application, multiple rising dose study of healthy subjects 
(n=24) receiving ZYN002 (200, 250, 500 mg/day), and patients with epilepsy (n=12), 
receiving 500 mg/day showed that ZYN002 was safe and well tolerated at all doses.  One 
subject receiving placebo discontinued due to an SAE, a device related infection (catheter) 
not related to trial drug.  Most TEAEs were mild in intensity and there was only one severe 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
33 
TEAE of back pain in a healthy subject administered ZYN002 500 mg.  Most TEAEs were 
considered related to trial drug and either resolved or were resolving.  Application site 
disorders were the most frequently reported TEAEs for both healthy subjects and epilepsy 
patients.  The most frequently reported TEAEs were application site dryness and application 
site pruritus.  Headache was the most frequently reported TEAE that was not associated with 
the application site.  There were no clinically significant changes in ECGs or vital signs. 
• 
 
 
  
 
 
  
• ZYN2-CL-08, a 14-day repeat application study in healthy subjects (n=42) receiving 
ZYN002 (394.8, 500, and 504 mg/day) or placebo showed that ZYN002 was safe and well 
tolerated at all doses.  Most TEAEs were mild in intensity.  Most TEAEs were considered not 
related to trial drug and either resolved or were resolving at the time of database lock.  No 
SAEs were reported and no subjects discontinued from the study due to a TEAE.  Headache 
and upper respiratory tract infection were the most frequently reported TEAEs and occurred 
in similar incidence for both ZYN002 and placebo.  Application site dryness, application site 
pain, and application site pruritus were the next frequently reported TEAEs.   
In the completed clinical pharmacology studies, ZYN002 was not associated with any 
impairment in critical areas of cognitive functioning often impacted by central nervous system 
(CNS) drugs, including divided attention and working memory and focused attention after single 
or repeat doses of ZYN002 at doses up to 250 mg as a single or BID dose.  Assessments of 
psychological health also demonstrated no changes in depression and anxiety symptoms, or 
positive and negative affect, following administration of ZYN002.   
5.3.3. 
ZYN002 Phase 2A Studies 
Two Phase 2A studies have completed in patients with epilepsy (ZYN2-CL-03) and 
osteoarthritis (OA) (ZYN-CL-005).   
• ZYN2-CL-03, a randomized, double-blind, placebo-controlled, multiple-dose study was 
conducted at 10 sites in Australia and 4 sites in New Zealand, to assess the efficacy and 
safety of ZYN002 administered as a transdermal gel to patients with focal epilepsy.  The 
results showed ZYN002 to be safe and well tolerated with a safety profile consistent with 
previous studies.  A total of 188 patients were randomized to receive either 195 or 390 
mg/day of ZYN002 or placebo for 12 weeks.  AEs were reported by 26 of 63 (41.3%) of 
placebo-treated patients, 31 of 63 (49.2%) of patients randomized to 195 mg/day of ZYN002 
and 32 of 62 (51.6%) of patients randomized to 390 mg/day of ZYN002.  For each dosing 
group, the majority of AEs were mild to moderate in intensity.  The TEAE that occurred 
more frequently in patients on drug than those on placebo and occurred in at least 2% of 
patients included: nausea, vomiting, abdominal pain, diarrhea, fatigue, application site 
dryness, application site pruritus, nasopharyngitis, urinary tract infection, thermal burn 
(secondary to seizure related accident), decreased appetite, headache, ataxia, anxiety, and 
oropharyngeal pain. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
34 
• ZYN2-CL-005, a randomized, double-blind, placebo-controlled, multiple center, multiple 
dose study, was conducted at 10 sites in Australia, to assess the efficacy and safety of a 12 
week repeat application in patients diagnosed with OA of the knee.  The results showed 
ZYN002 to be safe and well tolerated with a safety profile consistent with previous studies.  
A total of 320 patients were randomized to receive ZYN002 at 250 or 500 mg/day or placebo 
for 12 weeks.  Patients experiencing at least one treatment-emergent adverse event were 
similar between those on trial drug (n=106, 50%) and those on placebo (n=45, 42%).  The 
TEAEs that occurred more frequently in patients on drug than those on placebo and occurred 
in at least at 2% of patients included:  headache, dizziness, application site dryness, 
application site reaction, and application site pain. 
5.3.4. 
Ongoing ZYN002 Clinical Studies 
Two clinical studies are ongoing: 
• ZYN2-CL-009 is an ongoing open-label trial to assess the safety and efficacy of ZYN002 
administered as a transdermal gel to children and adolescents with FXS.  The trial is being 
conducted at three investigative sites in Australia.  The 12-week treatment period includes a 
6-week titration period, after which the patient is to remain on their maintenance dose over 
the next 6 weeks of treatment.  Following completion of the first 12 weeks of treatment, 
patients have the option of continuing into an extension phase of the trial, which allows for 
them to receive trial drug for up to 24 additional months.  The last patient is expected to 
complete the extension phase in September 2019. 
Twenty-two patients were screened for the study and 20 were enrolled.  Of the 20 patients, 
18 completed the 12 weeks of open-label treatment and 13 of those 18 patients continued into 
the extension phase of the study.  Currently, 12 patients are ongoing in the extension phase. 
Initial results of the 12-week treatment period include: 
Safety Results: Seventeen patients reported 33 TEAEs in this open-label trial.  The majority 
of TEAEs were mild (25 [76%]) or moderate in intensity (8 [24%]) and were considered 
unrelated to treatment with CBD (25 [76%]).  There were no SAEs reported, and 1/20 
patients (5%) discontinued treatment due to a TEAE. 
The most common treatment emergent adverse event was mild-moderate gastroenteritis (6 
[18%]), not related to trial drug and resolved during the study period.  This was followed by 
upper respiratory tract infection, viral infection, influenza, otitis media, and tonsillitis (7 
[21%], not related to trial drug and all resolved during the study period.  One patient (129-
003) developed a moderate application rash 35 days after starting trial drug.  Trial drug was 
withheld one day and the application site was changed to the thighs for 6 days while the rash 
resolved.  The rash did not recur.  This patient also had a high eosinophil count, considered 
probably related to the skin rash.  Upon a repeat laboratory test, the eosinophil count had 
decreased to just above normal.  This patient currently is in the extension phase of the study. 
Other adverse events considered possibly related included symptoms of FXS (e.g., sensorial 
hyperactivity, nightmares, increased bedwetting and increase in self stimulatory talk). 
There were no clinically significant changes in vital signs, ECG, clinical laboratory results 
(other than eosinophilia noted above), and no reports of suicidal ideation for any patient 
throughout the trial. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
35 
Efficacy Results: Eighteen patients completed the efficacy assessments for this study through 
Week 12.  Validated clinician- and caregiver-rated FXS scales were used to measure 
Baseline symptom severity and changes in symptoms between Baseline and Week 12 of the 
treatment period in this open-label trial. 
The primary endpoint was the change from Baseline to Week 12 in the total score of the 
ADAMS, a caregiver-rated scale.  Compared to the Baseline total score, the ZYN002 treated 
patients had a 45.81% reduction (p<0.0001) in the ADAMS Total Score.  Furthermore, 
ZYN002 treated patients had statistically and clinically significant improvement compared to 
Baseline in all but one of the ADAMS subscales (i.e., Manic/Hyperactive Behavior, Social 
Avoidance, General Anxiety, and Compulsive Behavior) at Week 12.  A significant change 
was not observed for the Depressed Mood subscale of the ADAMS.  For other assessments, 
changes representative of clinically meaningful changes were observed, including in the 
ABC-FXS, PARS-R, and Visual Analog Scale (VAS). 
ZYN2-CL-004 is an ongoing open-label continuation study to allow patients with focal epilepsy 
who completed ZYN2-CL-03 to continue to receive ZYN002.  The primary objective is to assess 
the long-term safety and tolerability of ZYN002 in adult epilepsy patients over an 18-month 
period.  The secondary objective is to evaluate efficacy in this population.  Patients had to 
complete the 12 weeks of study treatment on protocol ZYN2-CL-03.  ZYN002 is being 
administered as a transdermal gel with all patients starting on ZYN002 at doses equal to or 
higher than those used in the blinded study – CBD 195 mg every 12 hours (Q12 H) (+2 hours) 
(390 mg daily), with the option that after Month 1 to either increase or reduce the dose of 
ZYN002.  The dose may be increased to 292.5 mg Q12H (+2 hours) (585 mg daily).  After one 
month at the 585 mg daily dose, the Investigator has the option to increase the dose to 390 mg 
Q12H (+ 2 hours) (780 mg daily).  Of the patients who completed the blinded 12-week phase, 
171 (98%) enrolled in the open-label extension study.  Results from patients who had completed 
3 (n=169) and 6 (n=63) months of treatment as of August 15, 2017, showed an approximate 
seizure reduction ranging from 30% (Month 3) to 65% (Month 6).  The un-blinded use of 
ZYN002 for an additional three and six months appeared to result in clinically meaningful 
seizure reductions and was not due to an increase or addition of other AEDs. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
36 
6. 
TRIAL OBJECTIVES AND PURPOSE 
6.1. 
Primary Objective 
To evaluate the efficacy of ZYN002 administered as a transdermal gel formulation, for up to 12 
weeks, in patients ages 3 to < 18 years, in the treatment of symptoms of Fragile X Syndrome.  
The primary endpoint is the change from Baseline to Week 12 in the ABC-CFXS Social 
Avoidance subscale score.    
6.2. 
Secondary Objectives 
To further evaluate the efficacy of ZYN002 in the treatment of symptoms of FXS. 
To evaluate the safety of ZYN002 in the treatment of symptoms of FXS.  
To evaluate CBD, CBD metabolite and THC exposure. 
 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
37 
7. 
INVESTIGATIONAL PLAN 
7.1. 
Overall Study Design 
This is a randomized, double-blind, placebo-controlled, multiple-center study, to assess the 
efficacy and safety of CBD administered as ZYN002, a transdermal gel, for the treatment of 
child and adolescent patients with FXS.  Male and female patients with FXS will be treated for 
12 weeks with a 
 single-blind placebo lead-in preceding the 12-week double-blind 
treatment period.  Approximately 204 male and female patients, ages 3 to < 18 years, will be 
randomized 1:1 to either trial drug or placebo to attain a sample size of 102 subjects in each 
treatment arm.  Randomization will be stratified by gender, weight category and region. 

Protocol ZYN2-CL-016.05 
26 April 2020 
Confidential 
38 
Figure 2:  Study Design 
 

Protocol ZYN2-CL-016.05 
26 April 2020 
Confidential 
39 
7.2. 
Number of Patients 
Approximately 204 male and female patients will be randomized 1:1 to either trial drug or 
placebo.  No more than 25% of the patients screened will be females. 
7.3. 
Dose Rationale 
 
 
 
 
 
  
7.4. 
Dose Schedule 
Following the Screening period, eligible patients will start a single-blind Placebo Lead-In period 
for 
.  The placebo will be applied Q12H (± 2 hours).  For patients < 35 kg, each 
application will consist of one sachet of placebo containing 2.98 g of gel (two sachets in total per 
day).  For patients > 35 kg, each application will consist of two sachets of placebo containing 
2.98 g of gel in each sachet (four sachets in total per day). 
At Visit 3, patients will be randomized to receive either ZYN002 or placebo.  In a blinded 
fashion, ZYN002 treated patients who weigh < 35 kg will receive 125 mg CBD Q12H 
(± 2 hours); total daily dose of 250 mg CBD.  Patients who weigh > 35 kg will receive 250 mg 
CBD Q12H (+2 hours); total daily dose of 500 mg CBD.  All patients will remain on their 
assigned dose during the 12 weeks of the treatment phase of the study.  Study visits will occur at 
Week 4 (Visit 4), Week 8 (Visit 5), and Week 12 (Visit 6).  Patients who have successfully 
completed the 12 weeks of the double-blind study will have the option to enroll in an OLE study.  
Patients not taking AEDs and not continuing in the OLE study will complete their EOS 
procedures at Visit 6/ET.  Patients on AEDs who are not continuing in the OLE study will taper 
off trial drug and then have their EOS Visit.  Telephone Follow-Up calls will occur during the 
taper period and weekly for all patients who do not enter the OLE study for four weeks post the 
last dose of trial drug.  At Visit 2, parents/caregivers will be instructed on proper application of 
the study gel (active/placebo gel).  
 
  Parents/caregivers will apply all trial drug to clean, dry, 
intact skin, thoroughly massaging it into the right and/or left upper arms and shoulders until the 
area is dry.  The study gel will be rubbed in completely and must be dry prior to dressing.  
 
  The application site should 
be covered to minimize sun exposure when going outside during the day.  Parents/caregivers will 

Protocol ZYN2-CL-016.05 
26 April 2020 
Confidential 
40 
use gloves supplied by the Sponsor to apply the study gel.  If redness occurs at the application 
site, the parent (after consultation with the Investigator) may switch the application site 
temporarily to the upper thighs.  
7.5. 
Criteria for Study Withdrawal 
Each parent/caregiver and patient has the right to withdraw the patient or his/herself from the 
study at any time without prejudice.  If a parent/caregiver or patient withdraws the patient or 
his/herself from the study, the reason(s) must be stated in the CRF, and a final evaluation of the 
patient should be performed. 
The Investigator may discontinue any patient’s participation if he or she feels it is necessary for 
any reason during the study.  The Investigator and Sponsor may discontinue any patient’s 
participation for any reason including: any adverse event, a positive endorsement on Question 4 
or 5 of the C-SSRS, clinically significant worsening in Fragile X symptoms, adverse change in 
any laboratory test, or failure to comply with the protocol.  Samples for a post-study laboratory 
profile and follow-up safety exams should be obtained as soon after patient discontinuation as 
possible. 
All effort will be made to ensure that the ET procedures will be completed at the time of 
discontinuation. 
Patients who discontinue after randomization and before study completion may be replaced at 
the Sponsor’s discretion. 
7.6. 
Study Assessments 
7.6.1. 
Overview of Study Assessments 
Study procedures will be performed as summarized in the study schematic presented in Table 3.  
As patients as young as 3 years old will be enrolled in this study, parents/caregivers will play a 
significant role in the clinical trial.  Most of the procedures will need to be performed by the 
parent/caregiver, including trial drug application and routine site visits (monthly) as required per 
the study protocol.  Parents/caregivers should make every attempt to bring the patient to their 
scheduled visits within the window allowed by the protocol.  If there are visits where it is 
impossible for a patient to attend the site visit, the parent/caregiver should contact the 
Investigator to determine if an exception can be made for a research nurse to visit the patient at 
home.   
During the Screening Period, the site staff will review the eligibility criteria of the patient, 
review any medications including OTC medications the patient is taking, obtain the patient’s 
medical history including their Fragile X Diagnosis, any seizure history and demographics, 
check their vital signs, perform an ECG (may be collected at either Screening or Visit 2), assess 
Tanner Stage, perform a physical and neurological exam and a skin assessment, obtain some 
blood and urine for analysis, and administer assigned scales.  Blood samples will be taken for 
hematology and chemistry testing, T testing (males only), pregnancy testing (if applicable), and 
CGG repeat analysis.  Patients will also have a pre-dose blood sample drawn for plasma levels of 
CBD and THC testing, and AEDs testing (for patients using AEDs).  Urine will be collected for 
a urinalysis and drug screen.  The scales administered include the ABC-CFXS, ADOS-2® 
(ADOS®-2 will not be administered at Screening if it has been administered in the prior 6 

Protocol ZYN2-CL-016.05 
26 April 2020 
Confidential 
41 
months and the results are available), CGI-S, C-SSRS, MWC, PWC-20, VABS-3.  The 
parent/caregiver will also be asked the following question “What are the five behavioral, 
emotional, or social problems that most impacted your son/daughter and his/her family in 
approximately the past year?”  This is referred to as the “Qualitative Caregiver reported 
Behavioral Problems Survey”.  Optionally, if an IQ test was performed previously, those results 
may be provided to the Sponsor.   
Patients and parents/caregivers will be required to visit the clinic at Week -2 (Visit 2), Study Day 
1/Week 1 (Visit 3), Week 4 (Visit 4), Week 8 (Visit 5), and Week 12 (Visit 6) for vital signs, 
ECG (may be collected at either Screening or Visit 2), concomitant medication check, physical 
and neurological exams, Tanner Stage assessment, skin check examination, AE review including 
seizure assessment if applicable, and completion of the following questionnaires and scales: 
ABC-CFXS, ADAMS, CGI-I, CGI-S, C-SSRS, Family Impact PedsQL TM, MWC, PWC-20. 
In addition, parents/caregivers will complete a Caregiver Global Impression of Severity at 
Visit 2, Visit 3, and Visit 6/ET.  Parents/caregivers will also complete a Caregiver Global 
Impression of Change at Visit 6/ET. 
Patients who have successfully completed the 12 weeks of the double-blind study will have the 
option to enroll in an OLE study.  Patients who do not enter the OLE study or who discontinue 
early will have a Telephone Follow-Up call weekly for four weeks following each patient’s last 
dose of trial drug.  The parent/caregiver will be contacted weekly via the telephone during the 
Follow-Up and complete the MWC and PWC-20.   
Site-specific supplemental protocols may be implemented in a subset of ZYN2-CL-016 patients. 

Protocol ZYN2-CL-016.05 
26 April 2020 
Confidential 
42 
 
Table 3:  Schedule of Assessments  
Screening 
Placebo 
Lead-In 
Double-Blind Treatment 
 
 
 
Screening Visit a 
(Week -4) 
Visit 2  
Placebo 
Lead-In 
(
 
b 
Visit 3 
(Study Day 1) 
Randomization c 
Visit 4 
(Week 4)  
 
Visit 5 
(Week 8)   
 
Visit 6 
(Week 12) / 
Early 
Termination 
(ET)  
 
Taper Period d, e 
End of 
Study Visit 
(EOS )  
Weekly 
Telephone 
Follow-Up e 
(Four 
Weeks) 
Un-
scheduled 
Visit(s) 
Days 
 to Day -
 
Days 
to 
Day 
 
Day 1 
(± 3 days) 
Day 28 
(± 3 days) 
Day 56 
(± 3 days) 
Day 84 
(± 3 days) 
(One-Two 
Weeks) 
(± 3 days 
after Taper 
Period) 
(± 3 days) 
Skin Check 
Follow-up 
Informed consent 
X 
 
 
 
 
 
 
 
 
 
Review eligibility criteria 
X 
X 
X 
 
 
 
 
 
 
 
Medical history & 
demographics 
X 
 
 
 
 
 
 
 
 
 
Concomitant Meds 
X 
X 
X 
X 
X 
X 
 
X 
X 
X 
Complete physical and 
neurological examination f 
X 
 
 
 
 
 
 
 
 
 
Targeted Physical and 
Neurological Examination 
 
 
 
 
 
X 
 
X 
 
 
Tanner Stage assessment g 
X 
 
 
 
 
X 
 
 
 
 
Vital signs h 
X 
X 
X 
X 
X 
X 
 
X 
 
X 
12-lead ECGi 
X 
 
X 
 
X 
 
X 
 
 
Laboratory tests and 
urinalysis 
X 
 
 
X 
 
X 
X 
 
 
CGG Repeat Analysis 
X 
 
 
 
 
 
 
 
 
Serum / urine pregnancy 
test j 
X 
 
 
X 
X 
X 
 
X 
 
 
Urine Drug Screen (UDS) 
X 
 
 
 
 
 
 
 
 
 
Fragile X Diagnosis 
X 
 
 
 
 
 
 
 
 
 
Trial drug application k 
 
X 
X 
X 
X 
 
X 
 
 
 
CBD, THC and AED Blood 
Samples l 
X 
 
 
X 
 
X 
 
 
 
 
Qualitative Caregiver 
reported Behavioral 
Problems Survey 
X 
 
 
 
 
 
 
 
 
 
Aberrant Behavior 
Checklist-FXS Specific 
(ABC-CFXS) 
X 
X 
X 
X 
X 
X 
 
 
 
 

Protocol ZYN2-CL-016.05 
26 April 2020 
Confidential 
43 
 
Screening 
Placebo 
Lead-In 
Double-Blind Treatment 
 
 
 
Screening Visit a 
(Week -4) 
Visit 2  
Placebo 
Lead-In 
(
 
b 
Visit 3 
(Study Day 1) 
Randomization c 
Visit 4 
(Week 4)  
 
Visit 5 
(Week 8)   
 
Visit 6 
(Week 12) / 
Early 
Termination 
(ET)  
 
Taper Period d, e 
End of 
Study Visit 
(EOS )  
Weekly 
Telephone 
Follow-Up e 
(Four 
Weeks) 
Un-
scheduled 
Visit(s) 
Days 
 to Day -
 
Days 
 to 
Day
 
Day 1 
(± 3 days) 
Day 28 
(± 3 days) 
Day 56 
(± 3 days) 
Day 84 
(± 3 days) 
(One-Two 
Weeks) 
(± 3 days 
after Taper 
Period) 
(± 3 days) 
Skin Check 
Follow-up 
Anxiety, Depression and 
Mood Scale (ADAMS) 
 
X 
X 
X 
X 
X 
 
 
 
 
Clinical Global Impression- 
Severity (CGI-S) 
X 
X 
X 
X 
X 
X 
 
 
 
 
Clinical Global Impression-
Improvement (CGI-I) 
 
 
X 
X 
X 
X 
 
 
 
 
Caregiver Global 
Impression of Severity 
 
X 
X 
 
 
X 
 
 
 
 
Caregiver Global 
Impression of Change 
 
 
 
 
 
X 
 
 
 
 
Family Impact PedsQL™ 
 
X 
X 
 
 
X 
 
 
 
 
Autism Diagnostic 
Observation Schedule® 
(ADOS®-2) m 
X 
 
 
 
 
 
 
 
 
 
Vineland Adaptive 
Behavior Scales™ (VABS-
3) 
X 
 
 
 
 
 
 
 
 
 
Columbia-Suicide Severity 
Rating Scale (C-SSRS) 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
Marijuana Withdrawal 
Checklist -Short form 
(MWC) 
X 
 
 
 
 
X 
X 
X 
X 
 
Penn Physician Withdrawal 
Checklist (PWC-20) 
X 
 
 
 
 
X 
X 
X 
X 
 
Skin assessment exam n 
X 
X 
 
 
 
 
 
 
 
 
Skin irritation check o 
 
X 
X 
X 
X 
X 
 
X 
 
X 
Skin irritation check diary 
(daily) o 
 
X 
X 
X 
X 
X 
X 
X 
 
X 
Adverse events 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
IQ Test Results (optional) p 
X 
 
 
 
 
 
 
 
 
 

Protocol ZYN2-CL-016.05 
26 April 2020 
Confidential 
44 
 
 
Table 3 Footnotes 
a. 
The Screening visit procedures can be split into multiple visits for those patients whom the Investigator determines it would be difficult for the patient to 
undergo all Screening assessments on one day.  The first part of the Screening procedures will include eligibility criteria.  
b. If Visit 2 is split into multiple days, ABC-CFXS, ADAMS, CGI-S and Family Impact PedsQL™ should be performed on the same day. 
c. 
Patients will receive blinded study medication for the month between scheduled visits.  Medication dispensed at Visit 3 will include either four weeks of 
placebo OR four weeks of 125 mg Q12H OR four weeks of 250 mg Q12H.   
d. For Patients taking concomitant AEDs and not continuing in the OLE or are discontinuing prematurely: At Visit 6/ET, patients < 35 kg will be reduced, in a 
blinded manner, total daily dose of 125 mg, over one week.  For patients > 35 kg, receiving 500 mg daily dose, the dose of trial drug will be reduced in a 
blinded manner over two weeks.  During the first week of taper, the dose will be reduced to 125 mg Q12H; (ZYN002 or placebo) (+2 hours); total daily dose 
of 250 mg, followed by a second week of taper from 250 mg total daily dose to total daily dose of 125 mg.  After the one- or two-week taper, patients will 
discontinue from the study and attend EOS Visit.    
e. 
The Weekly Telephone Follow-Up calls will occur during the taper and weekly for four weeks post patient’s last dose of trial drug for those patients not 
continuing on to the open-label extension study.  
f. 
Complete physical and neurological exam including height and weight at screening.  Targeted physical and neurological exam at Visit 6/ET.  
g. Tanner Stage assessment performed on adolescent patients 10 to less than 18 years of age or earlier if clinically indicated by onset of menarche or other signs 
of precocious puberty. 
h. Vital signs include blood pressure, heart rate, respiratory rate, oral, infrared red forehead or tympanic temperature and will be recorded after the patient has 
been sitting for at least 5 minutes.  Vital signs will be taken prior to blood draws.  No food or drink is allowed 30 minutes before vital sign assessment.  
i. 
The ECG scheduled for collection at Screening may be completed at the Screening Visit or Visit 2. 
j. 
A serum pregnancy test will be completed at screening for females of childbearing potential.  A serum or urine pregnancy test will be completed at Visits 4, 
5, and 6 for females of childbearing potential. 
k. At Visit 2, all patients will receive placebo for 
.  At Visit 3, patients will be randomized to receive placebo or ZYN002.  No trial drug will be 
administered on the patient’s last day of study.  If the patient is continuing into the OLE study, they will receive the first dose of OLE trial drug on the last 
day of the ZYN2-CL-016 study.  On days of study visits, the trial drug will be applied at the study site during the visit. 
l. 
Blood will be collected for CBD and THC samples and AED plasma samples (for patients taking AEDs) pre-AED dose at Screening, pre-dose at Visit 4, and 
Visit 6/ET. Only patients currently taking AEDs will have blood samples for AED levels drawn.  Time of the last trial drug dose (if applicable) and AED 
dose (if applicable) will be recorded.  Time of blood collection will also be recorded. 
m.  ADOS®-2 will not be administered at Screening if it has been administered in the prior 6 months and the results are available. 
n. Right and left upper arms and shoulders will be examined to determine there are no imperfections, lesions, or discolorations where trial drug could be 
applied.  
o. A complete skin irritation check/ examination of the patient’s application sites for irritation will be completed daily by parent/caregivers and at pre-dose by 
the site staff at every visit through the study and any unscheduled visits for skin irritation follow-up.  When redness exists, efforts will be made to apply the 
gel to a non-red area of the shoulders and upper arms.  If the skin check score is higher than ‘2’ (moderate erythema) at any time, the caregiver will contact 
the site to determine if an unscheduled visit is required.  If redness occurs at the application site, the parent (after consultation with the Investigator) may 
switch the application temporarily to the upper thighs.  A de-identified photograph of the skin finding (or area) of interest may be taken after consultation 
with and approval of the Sponsor. 
p. Please provide results of patient’s IQ Test if this is available and is current within the past 5 years. 
 
 
 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
45 
 
7.6.2. 
Informed Consent 
Signed informed consent will be obtained at Screening from parent/caregivers and if applicable assent 
will be obtained from patients where developmentally appropriate.  The informed consent form (ICF) 
will be signed by the parent/caregiver and the assent by the patient (if applicable) before any study 
procedures are undertaken.  Details about how the ICF will be obtained and documented are provided in 
Section 17.3. 
7.6.3. 
Fragile X Diagnosis 
Patients must have a diagnosis of FXS through molecular documentation of FMR1 full mutation, at 
Screening.  
7.6.4. 
Optional IQ Test Results 
If the patient has had an IQ Test within the past 5 years, the Investigator may choose to include this at 
Screening.  If this is not available, the Investigator should indicate this in the CRF. 
7.6.5. 
Demographics 
At Screening, patient demographic information will be collected and recorded in the CRF.  No more 
than 25% of the patients screened will be females. 
7.6.6. 
Medical History 
A complete medical history will be obtained for each patient at Screening.   
7.6.7. 
Vital Signs 
Vital sign determinations, including sitting blood pressure, heart rate, respiratory rate, and oral, infrared 
forehead or tympanic body temperature, will be recorded at each study visit and any unscheduled visits.  
Vital signs will be recorded after the patient has been sitting for at least 5 minutes.  No food or drink is 
allowed 30 minutes before vital sign assessment.  Vital signs will be assessed prior to dosing and will be 
taken before any blood sample collection at all visits. 
7.6.8. 
Concomitant Medication Review 
Medication (prescription and OTC) use will be completed at Screening.  A review of patient 
concomitant medication will be performed at all study visits and any unscheduled visits. 
7.6.9. 
Adverse Event Review 
A review of AEs will be performed at all study visits and any unscheduled visits.  
Detailed information regarding AEs can be found in Section 12.2. 
7.6.10. 
Complete and Targeted Physical and Neurological Examinations 
A complete physical and neurological examination, including height and weight will be performed at the 
Screening Visit.  A brief targeted physical (including heart, lungs, abdomen, extremities, and body 
weight) and neurological examination (mental status, gait/cerebellar testing, extraocular movements, and 
reflexes with additional areas depending on patient) will be performed at Visit 6/ET and the EOS Visit 
(if applicable).  Any clinically significant changes will be documented. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
46 
 
Patient weight will be collected with minimal clothing (e.g., no coats, shoes, jumpers, or jackets). 
A complete physical examination will only be performed at Visit 6/ET if considered clinically relevant. 
7.6.11. 
Tanner Stage Assessment 
The pubic hair growth (both sexes), genital (males only) and breast (females only) development of all 
adolescent patients (i.e., 10 to less than 18 years of age at the time at entry, or earlier if clinically 
indicated by onset of menarche or other signs of precocious puberty) will be assessed using Tanner 
Staging (Appendix 21.1).  The assessments will be made by physical examination at Screening and Visit 
6/ET. Patients will be assigned a value under each category of Pubic Hair Growth (both sexes), Genitals 
(male patients only), and Breasts (female patients only).  Once a patient reaches a score of ‘5’, the 
examination need not be performed again. 
7.6.12. 
Electrocardiogram 
A 12-lead resting ECG will be obtained at Screening (may be collected at either Screening or Visit 2), 
Visit 4, Visit 6/ET, and the EOS Visit (if applicable).  As applicable, ECGs will be conducted pre-dose, 
within 60 minutes of trial drug application.  A qualified physician will interpret, sign, and date the 
ECGs.  Only clinical interpretations (normal, abnormal but not clinically significant, or abnormal and 
clinically significant) will be recorded in the CRF.  ECGs will be reviewed by a central, independent 
reader and abnormal ECGs will be reviewed by the Zynerba Medical Monitor. 
At the discretion of the investigator, patients will be allowed a short acting sedative (such as midazolam) 
to assist with the collection of ECG’s. 
7.6.13. 
Clinical Laboratory Testing 
All blood samples will be collected and handled in accordance with the instructions from the central 
laboratory.  For collection of laboratory samples, patients should fast for approximately eight hours prior 
to having blood drawn for blood laboratory analysis.   
All clinically significant abnormal laboratory test results will be followed to a satisfactory resolution.  
Instructions regarding the collection, processing, and shipping of these samples will be provided by the 
laboratory chosen for this study. 
Samples will be collected based on Table 3 Schedule of Assessments.  Routine laboratory tests (clinical 
chemistry and hematology) and urinalysis will be collected at Screening, Visit 4, Visit 6/ET, and the 
EOS Visit (if applicable).  
At the discretion of the investigator, patients will be allowed a short acting sedative (such as midazolam) 
to assist with the collection of blood samples. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
47 
7.6.13.1. Screening, Visit 4, Visit 6/ET and EOS Laboratory Assessments 
Table 4:  Laboratory Assessments 
Laboratory Testing 
Screening 
Visit 4, Visit 6/ET, 
EOS 
Chemistry 
Glucose 
X 
X 
Total bilirubin 
X 
X 
serum glutamic oxaloacetic 
transaminase/aspartate 
transaminase (SGOT/AST) 
X 
X 
serum glutamic pyruvic 
transaminase/alanine 
transaminase (SGPT/ALT) 
X 
X 
Alkaline Phosphatase 
X 
X 
blood urea nitrogen (BUN) 
X 
X 
Creatinine 
X 
X 
Amylase 
X 
X 
Total Protein 
X 
X 
Uric Acid 
X 
X 
Sodium 
X 
X 
Chloride 
X 
X 
Bicarbonate 
X 
X 
Potassium 
X 
X 
Calcium 
X 
X 
Phosphorus 
X 
X 
Albumin 
X 
X 
Triglycerides 
X 
X 
Cholesterol: low density 
lipoprotein (LDL) 
X 
X 
Cholesterol: high density 
lipoprotein (HDL) 
X 
X 
Testosterone (males only)a 
Total 
X 
X 
Free 
X 
X 
Hematology 
White blood cell (WBC) with 
differential count 
X 
X 
Red blood cell (RBC) 
X 
X 
Hematocrit 
X 
X 
Hemoglobin 
X 
X 
Platelet Count 
X 
X 
Hep & HIVb 
HBsAg 
X 
 
HCV 
X 
 
HIV 
X 
 
Urine c 
Specific Gravity 
X 
X 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
48 
Laboratory Testing 
Screening 
Visit 4, Visit 6/ET, 
EOS 
pH 
X 
X 
Protein 
X 
X 
Glucose 
X 
X 
Ketones 
X 
X 
Bilirubin 
X 
X 
Blood 
X 
X 
Leukocyte esterase  
X 
X 
Nitrite 
X 
X 
Drug Screen b 
X 
 
a. 
Testosterone should be collected at the same time of day throughout the study. 
b. Hep & HIV testing and Drug Screen performed at Screening Visit Only. 
c. 
Urine microscopic analysis if indicated. 
7.6.14. 
Serum and Urine Pregnancy Tests 
A serum pregnancy test will be performed for female patients of childbearing potential at the 
Screening Visit.  A serum or urine pregnancy test will be performed for female patients of 
childbearing potential on Visit 4, Visit 5,Visit 6/ET, and the EOS Visit (if applicable).  Any 
patient who becomes pregnant will be excluded or discontinued from the study, as applicable.  
The Investigator will advise on further medical attention, should this be necessary.  The 
Investigator will ask for the patient’s or caregiver’s consent to follow the progress of the 
pregnancy and the birth and the health of the child.  In the event of a positive pregnancy test in a 
female participant, where the caregiver may not be aware of sexual activity, the parent/caregiver 
will be notified of a confirmed positive pregnancy test.  The Investigator will refer the patient 
and her parent(s)/caregiver to an appropriate obstetrician/gynecologist with relevant expertise for 
further management, following discussion of family planning and counseling options.   
7.6.15. 
Skin Assessment Examination 
At Screening and Visit 2, the shoulders and upper arms will be examined to determine there is no 
skin disease or condition, including eczema, psoriasis, melanoma, acne, contact dermatitis, 
scarring, imperfections, lesions, tattoos, or discoloration, that may affect treatment application, 
application site assessments, or absorption of the trial drug..  If there is irritation on the shoulders 
and upper arms, an assessment of the upper thighs will be made to determine if the upper thighs 
can be an alternative application site. 
7.6.16. 
Skin Irritation Check Examination 
Parents/caregivers will use a diary to complete a daily skin irritation check examination 
(Appendix 21.2).  This diary will be reviewed at each study visit including any unscheduled 
visits.  Every evening, they will record the skin irritation check score in the daily skin irritation 
check diary. 
A complete skin check examination will be conducted by the Investigator at each study visit starting 
with Visit 2, including Unscheduled Visits and the End of Study Visit (if applicable) for skin redness 
follow-up. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
49 
When skin redness is noted, parents/caregivers should apply the gel to a non-red area of the 
shoulders and upper arms.  If the skin irritation check score is higher than ’2’ at any time, the 
parent/caregiver will contact the study site to determine if an Unscheduled Visit is required.  The 
Investigator will use discretion in suspending dosing for patients with a skin irritation check 
score of ‘4’ but will, in all cases, immediately (within 24 hours) complete an adverse event report 
and contact their study CRA and the Zynerba Medical Monitor.  A de-identified photograph of 
the skin finding (or area) of interest may be taken after consultation with and approval of the 
Sponsor.  Digital photographs will be retained for information purposes only.  If redness occurs 
at the application site, the parent (after consultation with the Investigator) may switch the 
application temporarily to the upper thighs. 
Refer to Table 5 for the Skin Irritation Check Scale to be used for skin irritation examinations. 
Table 5:  Skin Irritation Check Scale 
Score 
Definition 
0 
No erythema 
1 
Minimal erythema 
2 
Moderate erythema with sharply defined 
borders 
3 
Intense erythema with or without edema 
4 
Intense erythema with edema and 
blistering/erosion 
 
7.6.17. 
Blood Samples for AEDs, CBD and THC Levels 
Blood samples for plasma levels of CBD and THC will be collected in all patients at the 
Screening Visit, Visit 4, and Visit 6/ET.  Plasma may also be analyzed for CBD metabolite 
concentrations.  In addition, pre-dose blood samples for adjunctive AED blood levels (for 
patients taking AEDs) will be collected at Screening and Visit 4, and Visit 6/ET.  The times of 
blood sample collection, as well as the times of last dose (AED and trial drug) will be recorded.  
Plasma samples will be analyzed by
 
  Plasma samples for adjunctive AEDs will be 
analyzed through a commercial laboratory.   
At the discretion of the investigator, patients will be allowed a short acting sedative (such as 
midazolam) to assist with the collection of blood samples. 
7.6.18. 
Blood Samples for CGG Analysis 
Blood samples will be collected during the Screening Visit (although not required for inclusion) 
for analysis of CGG repeat size, as well as methylation status.  Whole blood will be collected 
and stored by the Central Laboratory at -20℃ and will be batch shipped periodically to the 
analytical laboratory selected by the Sponsor. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
50 
 
 
 
 
 
 
 
 
 
At the discretion of the investigator, patients will be allowed a short acting sedative (such as 
midazolam) to assist with the collection of blood samples. 
7.6.19. 
Assessments of FXS Symptomatology 
The same site rater should be used for all clinician-reported assessments across all visits.  The 
same parent/caregiver should be used for all parent/caregiver-reported assessments across all 
visits but at minimum Visits 3 and 6/ET. 
7.6.19.1. Aberrant Behavior Checklist-Community FXS Specific (ABC-CFXS)  
The Aberrant Behavior Checklist- Community, 2nd Edition, will be scored using the FXS-
specific factoring system (ABC-CFXS).  The ABC-CFXS will be completed by the 
parent/caregiver, with support from the site staff, at Screening, Visit 2, Visit 3, Visit 4, Visit 5, 
and Visit 6/ET.  The ABC-CFXS asks responders to rate behaviors from “0 - not at all a problem” 
to “3 - the problem is severe in degree” across 58 questions.  Use of the checklist has been 
validated in a variety of clinical populations, including in FXS.  Scores will be analyzed using 
the FXS-specific factor structure such that 55 of the items resolve into 6 subscales (Irritability, 
Socially Unresponsive/Lethargic, Social Avoidance, Stereotypy, Hyperactivity, and 
Inappropriate Speech; Sansone et al. 2012). 
7.6.19.2. Autism Diagnostic Observation Schedule® (ADOS®-2) 
The ADOS (Western Psychological Services) is a semi-structured assessment of communication, 
social interaction and play or imaginative use of materials for individuals suspected of having 
Fragile X or other pervasive developmental disorders from 12 months through adulthood (Lord 
et al. 2000).  The ADOS®-2 consists of various activities to allow the observation of social and 
communication behaviors related to the diagnosis of pervasive developmental disorders.  The 
ADOS®-2 includes five modules for use with different age groups and language level.  In this 
version, a Toddler Module has been included. For this patient population, the appropriate module 
will be utilized based on age and language level. Each subject will be administered one module.  
The assessment is administered by a certified rater and requires 35 to 40 minutes to complete.  
The ADOS®-2 is a diagnostic tool and this information will be used to document the presence of 
autism or other pervasive developmental disorders.  This assessment will only be administered at 
sites with qualified individuals based on the publisher’s criteria.  The ADOS®-2 will be 
completed at Screening unless it has been administered within the prior 6 months and the results 
are available. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
51 
7.6.19.3.  Anxiety, Depression, and Mood Scale (ADAMS)  
The Anxiety, Depression, and Mood Scale will be completed by the parent/caregiver, with 
support from the site staff, at Visit 2, Visit 3, Visit 4, Visit 5, and Visit 6/ET.  The ADAMS will 
be used as a comprehensive assessment of anxiety, depression, and mood among the FXS 
patients.  The ADAMS is comprised of 28 items, which are rated on a scale of “0 - not a 
problem” to “3 - severe problem.”  The ADAMS yields a total score as well as five subscale 
scores:  “Manic/Hyperactive Behavior,” “Depressed Mood,” “Social Avoidance,” “General 
Anxiety,” and “Compulsive Behavior.”  The ADAMS has been validated in clinical FXS 
populations, and has demonstrated good internal consistency and test-retest reliability.  Interrater 
reliability has been shown to be satisfactory (Esbensen et al. 2003). 
7.6.19.4. Clinical Global Impressions Scale-Severity and Improvement 
The Clinical Global Impression Scales, Severity (CGI-S) and Improvement (CGI-I), are 
commonly used in clinical trials (Leigh et al. 2013) as they allow the clinician to utilize the 
history from the caregiver and incorporate the score into a clinical rating for the severity of 
symptoms.  CGI-S will be assessed at Screening, Visit 2, Visit 3, Visit 4, Visit 5, and Visit 6/ET 
to judge the severity of the symptoms of FXS at the onset of and throughout the study.  CGI-I 
will be assessed at Visit 3, Visit 4, Visit 5, and Visit 6/ET.   
CGI-S is a 7-point scale that requires the clinician to rate the severity of the patient's illness at 
the time of assessment, relative to the clinician's past experience with patients who have the 
same diagnosis.  Considering total clinical experience, a patient is assessed on severity of mental 
illness at the time of rating 1 - normal, not at all ill; 2 - borderline mentally ill; 3 - mildly ill; 4 - 
moderately ill; 5 - markedly ill; 6 - severely ill; or 7 - extremely ill. 
CGI-I is a 7-point scale that requires the clinician to assess how much the patient's illness has 
improved or worsened relative to a Baseline state at the beginning of the intervention and rated 
as: 1 - very much improved; 2 - much improved; 3 - minimally improved; 4 - no change; 5 - 
minimally worse; 6 - much worse; or 7 - very much worse.  Information from both the clinician 
and the parent/caregiver history are incorporated into a clinical rating. 
7.6.19.5. Caregiver Global Impression of Severity and Change Scales 
Using a Caregiver Global Impression of Severity scale, parents/caregivers will be asked to 
complete a 
 scale rating the problems their child may be having with 
 
, and the child’s behavior overall at Visit 2, 
Visit 3, and Visit 6/ET.  Using the Caregiver Global Impression of Change scale, 
parents/caregivers will be asked at Visit 6/ET to complete 
 
for the same questions asked in the Caregiver Global Impression of 
Severity scale.   
7.6.19.6. Family Impact PedsQLTM (Pediatric Quality of Life Inventory) 
 
The Family Impact PedsQL™ includes evaluations that are completed by the parent/caregiver.  
The scale will be completed at Visit 2, Visit 3, and Visit 6/ET.  The newly developed PedsQL™ 
Family Impact Module is a 36-item scale, designed to measure the impact of pediatric chronic 
health conditions on parents and the family.  The PedsQL™ Family Impact Module measures 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
52 
parent self-reported physical, emotional, social, and cognitive functioning, communication, and 
worry.  The Family Impact Module also measures parent-reported family daily activities and 
family relationships (Varni et al. 2004).  
7.6.19.7. Vineland Adaptive Behavior Scales™, Third Edition (VABS-3) 
The VABS-3 (Sparrow et al. 2016) was designed to assess adaptive functioning from birth to age 
90.  The VABS-3 includes a comprehensive structured interview administered to a 
parent/caregiver, within which four key domains are assessed: Communication, Daily Living 
Skills, Socialization, and Motor Skills, as well as Maladaptive Behavior (i.e., internalizing and 
externalizing).  The scale has been successfully used in clinical trials of pharmaceuticals in 
children and adolescents with FXS (Berry-Kravis et al. 2017).  The Adaptive Behavior 
Composite (a total score), the Communication, Daily Living Skills, and Socialization domains, 
as well as Maladaptive Behavior, will be evaluated in the present study.  The VABS-3 will be 
completed at Screening to assess baseline adaptive functioning. 
7.6.20. 
Columbia Suicide Severity Rating Scale (C-SSRS) (Children’s Version) 
The C-SSRS (Children’s version) is to be completed at Screening, all study visits and any 
unscheduled visits.  The C-SSRS assessment will be conducted only if the patients are of an 
appropriate age (6 years or older) and capable of understanding and answering the questions in 
the Investigator’s opinion.  For patients under the age of 6 or who are not capable of 
understanding and answering the questions in the Investigator’s opinion, the Investigator will 
consider the trend in ABC-Irritability when assessing the risk of self-harm for an individual 
patient.  Note that any completed suicide or suicidal attempt will be collected as an SAE.  
7.6.21. 
Marijuana Withdrawal Checklist - Short form (MWC, Behavior Checklist) 
The 15-item Marijuana Withdrawal Checklist – Short Form (Budney et al. 1999; 2003) 
retrospectively assesses cannabis withdrawal symptoms experienced during the past week.  The 
symptoms included in the MWC consists of validated cannabis withdrawal symptoms: depressed 
mood, irritability, nervousness/anxiety, restlessness, increased aggression, increased anger, 
nausea, decreased appetite, stomach pains, shakiness/tremulousness, sweating, sleep difficulty, 
strange/wild dreams, craving to smoke marijuana, and headaches.  The clinician will interview 
the parent/caregiver (or patient, if applicable), to rate each of the 15 symptoms on a four-point 
Likert-type scale (0=none, 1=mild, 2=moderate, 3=severe).  A composite Withdrawal 
Discomfort Score (WDS) is calculated as the sum of the MWC items known to be valid, reliable 
cannabis withdrawal symptoms (American Psychiatric Association 2013). 
The MWC is to be completed at Screening, Visit 6/ET, during the Taper Period, the EOS Visit, 
and during the four-week Weekly Telephone Follow-up calls.  
7.6.22. 
Penn Physician Withdrawal Checklist (PWC-20) 
The Penn Physician Withdrawal Checklist (PWC-20; Rickels et al. 2008) is a 20-item instrument 
developed to assess anxiolytic discontinuation symptoms.  The clinician will interview the 
parent/caregiver (or patient, if applicable) to rate the presence/severity of 20 common anxiolytic 
withdrawal symptoms (e.g., insomnia, diarrhea, weakness) on a four-point Likert-type scale 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
53 
ranging from 0 (“Not present”) to 3 (“Severe”).  Individual item scores are summed to yield a 
total score, with higher scores indicative of more severe withdrawal symptoms. 
The PWC-20 is to be completed at Screening, Visit 6/ET, during the Taper Period, the EOS 
Visit, and during the four-week Weekly Telephone Follow-up calls.  
7.7. 
End of Study (EOS) Visit and Early Termination (ET) Visit 
Patients, who have successfully completed through Week 12 of the protocol and have been at least 
90% compliant with the trial drug and visits, have the opportunity to continue in an OLE Study. 
Patients not on AEDs who are not continuing in the OLE study or are prematurely discontinuing 
will complete the EOS procedures at Visit 6/ET.  See Table 3 Schedule of Assessment for 
procedures at Visit 6/ET. 
Patients taking concomitant AEDs who are not continuing in the OLE study or are prematurely 
discontinuing will taper down their study dose in the following manner at Visit 6/ET: 
• For patients weighing < 35 kg (250 mg daily dose), the dose of trial drug will be reduced, in 
a blinded manner, to a total daily dose of 125 mg ZYN002 or 2.98 g placebo gel each day 
for one week (one sachet each morning), after which time the patients will discontinue from 
the study and attend the EOS Visit.  See Table 3 Schedule of Assessments for procedures at 
the EOS Visit.  The parent/caregiver will be contacted weekly during the Taper Period and 
complete the MWC and PWC-20. 
• For those patients > 35 kg (500 mg daily dose), the dose of trial drug will be reduced, in a 
blinded manner over two weeks: during the first week of taper the dose will be reduced to 
125mg Q12H; (ZYN002 or placebo) (+2 hours); total daily dose of 250 mg, followed by a 
second week of taper from 250 mg total daily dose to a total daily dose of 125 mg ZYN002 
or 2.98g placebo gel each day (one sachet each morning).  After the taper, patients will 
discontinue from the study and attend the EOS Visit.  See Table 3 Schedule of Assessments 
for procedures at the EOS Visit.  The parent/caregiver will be contacted weekly during the 
Taper Period and complete the MWC and PWC-20. 
 
  
Patients who do not enter the OLE study or who discontinue early will have Telephone Follow-
Up calls weekly for four weeks following each patient’s last dose of trial drug.  The 
parent/caregiver will be contacted weekly via the telephone during the Follow-Up and complete 
the MWC and PWC-20.   

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
54 
8. 
SELECTION AND WITHDRAWAL OF PATIENTS 
Patients participating in this study will have a diagnosis of FXS through molecular 
documentation of FMR1 full mutation.  No more than 25% of the patients screened will be 
females.  Siblings may be screened and enrolled based upon Investigator discretion and 
discussion with the Medical Monitor (See section 8.3.1).  Patients must qualify based on 
completing all the inclusion criteria and none of the exclusion criteria to be eligible to enroll.   
8.1. 
Patient Inclusion Criteria 
1. 
Male or female children and adolescents aged 3 to <18 years, at the time of Screening.  
2. 
Judged by the Investigator to be in generally good health at Screening based upon the 
results of a medical history, physical examination, 12-lead ECG, and clinical laboratory 
test results.  Laboratory results outside of the reference range must be documented as 
acceptable by both the Investigator and Sponsor. 
3. 
Patients must have a diagnosis of FXS through molecular documentation of FMR1 full 
mutation. 
4. 
Patients with an ABC-CFXS 
 score of 
 at Screening OR patients with 
an ABC-CFXS 
 score of 
 at Screening with an ABC-CFXS 
 
score 
.   
5. 
Patients have a CGI-S score of at least ‘3’ at Screening.  
6. 
Patients with a history of seizure disorders must currently be receiving treatment with a 
stable regimen of one or two AEDs or must be seizure-free for one year if not currently 
receiving AEDs. 
7. 
Patients taking AEDs should be on a stable regimen for the four weeks preceding study 
Screening and taking no more than two.  
8. 
Patients who are taking psychotropic medication(s) should be on a stable regimen of no 
more than two such medications for at least four weeks preceding study Screening and 
must maintain that regimen throughout the study.  Psychotropic medications include (but 
are not limited to) antipsychotics, antidepressants, anxiolytics, and ADHD medications. 
9. 
If patients are receiving non-pharmacological behavioral and/or dietary interventions, they 
must be stable and have been doing so for three months prior to Screening. 
10. Patients have a body mass index between 12–30 kg / m2 (inclusive). 
11. Females of childbearing potential must have a negative serum pregnancy test at the 
Screening Visit and a negative serum or urine pregnancy test at all designated visits. 
12. Patients and parents/caregivers agree to abide by all study restrictions and comply with all 
study procedures. 
13. Patients and parents/caregivers must be adequately informed of the nature and risks of the 
study and give written informed consent (and assent if applicable) prior to Screening. 
14. Parents/caregiver(s) must provide written consent to assist in trial drug administration. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
55 
15. In the Investigator’s opinion, patients and parents/caregivers are reliable and willing and 
able to comply with all protocol requirements and procedures. 
 
8.2. 
Patient Exclusion Criteria 
Any of the following is considered criterion for exclusion: 
1. Females who are pregnant, nursing, or planning a pregnancy; females of childbearing 
potential and male patients with a partner of childbearing potential who are unwilling or 
unable to use an acceptable method of contraception as outlined below for the duration of 
therapy and for three months after the last dose of study medication. 
Standard acceptable methods of contraception include abstinence or the use of a highly 
effective method of contraception, including hormonal contraception, diaphragm, cervical 
cap, vaginal sponge, condom, spermicide, vasectomy, or intrauterine device. 
2. History of significant allergic condition, significant drug-related hypersensitivity, or 
allergic reaction to any compound or chemical class related to ZYN002 or its excipients. 
3. Exposure to any investigational drug or device < 30 days prior to Screening or at any time 
during the study. 
4. Patient has ALT, AST, or total bilirubin levels ≥ 2 times the ULN or has alkaline 
phosphatase levels ≥ 3 times the ULN as determined from Screening safety laboratories. 
5. Use of cannabis or any THC or CBD-containing product (aside from ZYN002) within 
three months of Screening Visit or during the study.  
6. Patient has a positive drug screen, including ethanol, cocaine, THC, barbiturates, 
amphetamines (unless prescribed), benzodiazepines (except midazolam or comparable 
administered for blood draws and ECG collection), and opiates.  
7. Patient is using the following AEDs: clobazam, phenobarbital, ethosuximide, felbamate, or 
vigabatrin.  
8. Patient is using any strong inhibitor/inducer of CYP3A4 or sensitive substrate for CYP3A4 
including the following medications: midazolam (except single doses administered for the 
purposes of obtaining blood samples and ECG’s), oral ketoconazole, fluconazole, 
nefazadone, rifampin, alfentanil, alfuzosin, amiodarone, cyclosporine, dasatinib, docetaxol, 
eplerenone, ergotamine, everolimus, fentanyl, halofantrine, irinotecan, lapatinib, 
levomethadyl, lumefantrine, nilotinib, pimozide, quinidine, ranolazine, sirolimus, 
tacrolimus, temsirolimus, toremifene, tretinioin, vincristine, vinorelbine, and St. John’s 
Wort.  
9. Patients may not be taking minocycline for 30 days prior to Screening or throughout the 
study. 
10. Patients may not be taking any benzodiazepines (except single doses administered for the 
purposes of obtaining blood samples and ECG’s) at screening or throughout the study. 
11. Patient has an advanced, severe, or unstable disease that may interfere with the study 
outcome evaluations. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
56 
12. Patient is expected to initiate or change pharmacologic or non-pharmacologic interventions 
during the course of the study. 
13. Patient has an acute or progressive neurological disease, psychosis, schizophrenia or any 
psychiatric disorder or severe mental abnormalities (other than Fragile X Syndrome) that 
are likely to require changes in drug therapy or interfere with the objectives of the study or 
the ability to adhere to protocol requirements. 
14. Patient has a positive result for the presence of HBsAg, HCV, or HIV antibodies. 
15. Patient has known history of cardiovascular disease, advanced arteriosclerosis, structural 
cardiac abnormality, cardiomyopathy, serious heart rhythm abnormalities, coronary artery 
disease, cardiac conduction problems, exercise-related cardiac events including syncope 
and pre-syncope, risk factors for Torsades de pointes (TdP) (e.g., heart failure, 
hypokalemia, family history of Long QT Syndrome), or other serious cardiac problems. 
16. Any clinically significant condition or abnormal findings at the Screening Visit that would, 
in the opinion of the Investigator, preclude study participation or interfere with the 
evaluation of the study medication. 
17. Any skin disease or condition, including eczema, psoriasis, melanoma, acne, contact 
dermatitis, scarring, imperfections, lesions, tattoos, or discoloration, that may affect 
treatment application, application site assessments, or absorption of the trial drug. 
18. History of treatment for, or evidence of, drug abuse within the past year. 
19. Previous participation in a ZYN002 study. 
20. Patient responds “yes” to Question 4 or 5 on the C-SSRS (Children) during Screening or at 
any time on study. 
8.3. 
Randomization Criteria 
After the Baseline Period, patients will qualify for randomization if they continue to meet the 
inclusion criteria and none of the exclusion criteria for the study.  Patients eligible for 
randomization will be either: 
• Patients with an ABC-CFXS 
 score 
 on Study Day 1 prior to 
randomization, who have
 
, OR  
• Patients with an ABC-CFXS 
 score of 
 with an ABC-CFXS 
 score 
 on Study Day 1 prior to randomization, who have
 
   
All inclusion and exclusion criteria must be reviewed for eligibility for randomization. 
8.3.1. 
Sibling Options 
Siblings may be screened and enrolled based upon Investigator discretion and discussion with 
the Medical Monitor.  After the discussion with the Medical Monitor and confirming eligibility, 
the Investigator has three options: 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
57 
Option 1: Siblings may be randomized at the same time and participate in the study (i.e., 
concurrent).  
Option 2: One sibling is randomized, completes the 12 weeks of treatment, at which point the 
next sibling is randomized (i.e., sequential). 
Option 3: Only one sibling is allowed to be enrolled and randomized.  No other siblings are 
allowed to participate. 
8.4. 
Patient Withdrawal Criteria 
Each parent/caregiver and patient has the right to withdraw the patient or him/herself from the 
study at any time without prejudice.  If a parent/caregiver or patient withdraws the patient or 
him/herself from the study, the reason(s) must be stated in the CRF, and a final evaluation of the 
patient should be performed. 
The Investigator may discontinue any patient’s participation if he or she feels it is necessary for 
any reason during the study.  The Investigator and Sponsor may discontinue any patient’s 
participation for any reason including: any adverse event, a positive endorsement on Question 4 
or 5 of the C-SSRS, clinically significant worsening in Fragile X symptoms, adverse change in 
any laboratory test, or failure to comply with the protocol.  All efforts will be made to follow-up 
adverse events until resolution.   
Samples for a post-study laboratory profile and follow-up safety exams should be obtained as 
soon after patient discontinuation as possible. 
All effort will be made to ensure that the End of Study procedures will be completed at the time 
of discontinuation. 
 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
58 
9. 
TREATMENT OF PATIENTS 
9.1. 
Description of Trial Drug 
ZYN002 is a pharmaceutically manufactured CBD in a clear permeation-enhanced gel 
formulation.  The drug product will be supplied as a transdermal gel and will be contained in a 
sachet.  The gel will be applied to clean, dry, intact skin of the shoulders and upper arms or on a 
case by case basis the upper thighs.  The drug product concentration is 4.2%. Dosing volume will 
be one (1) or two (2) sachets of 4.2% per day. 
Placebo will consist of a clear gel similar in appearance and texture to ZYN002.  The placebo 
will be supplied as a transdermal gel and will be contained in a sachet.  The gel will be applied to 
clean, dry, intact skin of the shoulders and upper arms or on a case by case basis the upper 
thighs.  Each application will consist of one or two sachets of placebo containing 2.98 g of gel 
applied Q12H (+2 hours).  
SINGLE-BLIND PLACEBO LEAD-IN PERIOD 
During the single-blind Placebo Lead-In period (
) patients < 35 kg will receive 
placebo, applied Q12H (± 2 hours).  Each application will consist of one sachet of placebo 
containing 2.98 g of gel (two sachets in total per day).  Patients > 35 kg will receive placebo, 
applied Q12H (+2 hours).  Each application will consist of two sachets of placebo containing 
2.98 g of gel in each sachet (four sachets in total per day). 
STUDY DAY 1, RANDOMIZATION 
At Visit 3 (Study Day 1, Randomization) patients will be randomized to either: 
Treatment A (Trial Drug)  
Patients < 35 kg will receive 125 mg CBD applied Q12H (± 2 hours); total daily dose of 250 mg 
CBD.  Each application will consist of one sachet of ZYN002 CBD 4.2% concentration, 
containing 2.98 g of gel.  This dosing will continue for the duration of the study. 
Patients > 35 kg will 250 mg CBD applied Q12H (+2 hours); total daily dose of 500 mg CBD.  
Each application will consist of two sachets of ZYN002 CBD 4.2% concentration, each 
containing 2.98 g of gel.  This dosing will continue for the duration of the study. 
OR 
Treatment B (Placebo) 
Patients < 35 kg will receive placebo, applied Q12H (+ 2 hours).  Each application (Q12H; + 2 
hours) will consist of one sachet of placebo, containing 2.98 g of gel.  This dosing will continue 
for the duration of the study. 
Patients > 35 kg will receive placebo, applied Q12H (+ 2 hours).  Each application (Q12H; +2 
hours) will consist of two sachets of placebo, each containing 2.98 g of gel.  This dosing will 
continue for the duration of the study. 
9.2. 
Concomitant Medications 
Because CYP3A4 and CYP2C19 are the major isoforms responsible for CBD metabolism, 
concomitant administration of drugs that inhibit these enzymes may result in higher exposure to 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
59 
CBD and drugs that induce these enzymes may result in lower exposure to CBD; therefore, 
strong inhibitors or inducers of CYP3A4 may increase or decrease the plasma concentrations of 
CBD and should be administered with caution. 
As a potential perpetrator, CBD would not cause a clinically significant induction of CYP 
isoenzymes.  However, CBD exhibited time-dependent inhibition of CYP2D6 and CYP1A2, 
which was reversible, and of CYP3A4, which was irreversible.  Sensitive CYP3A4 substrates 
with a narrow therapeutic index or risk for severe toxicity should be avoided until this risk has 
been assessed with ZYN002.  CBD did not inhibit human efflux and uptake transporters.  
Refer to the following site for examples of strong inhibitors/inducers of CYP 3A4 and CYP 3A4 
substrates with narrow therapeutic indexes:  
https://www.fda.gov/Drugs/DevelopmentApprovalProcess/DevelopmentResources/DrugInteracti
onsLabeling/ucm093664.htm. 
9.2.1. 
Concomitant Medications Allowed 
At the discretion of the investigator, patients will be allowed a short acting sedative (such as 
midazolam) to assist with the collection of blood samples and ECG’s. 
Patients may take hormonal contraception and AEDs (not in the exclusion criteria) during study 
participation.  Other prescription or over-the-counter medications may be taken as approved in 
advance by the Investigator and recorded in the CRF.  
AEDs may be added or discontinued as clinically indicated but patients on prior AEDs must 
remain on at least one concomitant AED in addition to ZYN002.  Only AEDs not listed in the 
exclusions may be used. 
9.2.1.1. 
Psychotropic Medications 
Patients may only take one medication each to treat psychosis, anxiety or ADHD but may not 
take more than two medications total to treat all three conditions. 
Psychotropic medications include (but are not limited to) antipsychotics, antidepressants, 
anxiolytics, and ADHD medications. 
Since there are no medications with an indication specific to Fragile X Syndrome, treatment is 
symptomatic and consists of medications approved for other indications.  The following clarifies 
which drug classes should be considered as psychotropic medications as they relate to inclusion 
number 8: 
• 
Antipsychotics 
• 
Antidepressants 
• 
Anxiolytics (including buspirone or anticonvulsants prescribed for anxiety)  
• 
Stimulants (e.g. methylphenidate) 
Patients should be taking no more than two of the medications in the above classes and be on a 
stable regimen for four weeks prior to screening. 
 
 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
60 
 
For the purposes of the protocol, the following sedative medications, when used as a single, 
bedtime dose – need not be considered one of the two allowed psychotropic medications: 
• 
Clonidine 
• 
Guanfacine 
• 
Melatonin 
• 
Diphenhydramine or other sedating antihistamine medications 
Clonidine or guanfacine administered in the morning or more frequently than once daily at 
bedtime would, however, be counted as one of the two permitted psychotropic medications.  
There are certain medications used for their sedative properties that may have more profound or 
long-lasting psychotropic effects, so these would need to be considered as one of the two 
permitted psychotropic medications, e.g.: 
• 
Quetiapine 
• 
Trazadone 
• 
Amitriptyline, or other tricyclic antidepressants 
Additionally, metformin need not be considered as a psychotropic medication for purposes of 
inclusion criterion number 8. 
9.2.2. 
Concomitant Medications Not Allowed 
The following medications are not allowed: midazolam*, oral ketoconazole, fluconazole, 
nefazadone, rifampin, alfentanil, alfuzosin, amiodarone, cyclosporine, dasatinib, docetaxol, 
eplerenone, ergotamine, everolimus, fentanyl, halofantrine, irinotecan, lapatinib, levomethadyl, 
lumefantrine, nilotinib, pimozide, quinidine, ranolazine, sirolimus, tacrolimus, temsirolimus, 
toremifene, tretinioin, vincristine, vinorelbine and St. John’s Wort.   
Patients may not take any benzodiazepines at screening or throughout the study*. 
* Except single doses administered for the purposes of obtaining blood samples and ECG’s. 
9.3. 
Treatment Compliance 
The Investigator will keep a current and accurate inventory of all clinical supplies received from 
the Sponsor.  Any deviations from the protocol will be recorded.  
All parents/caregivers will be provided with a sufficient supply of trial drug for the patient 
during their site visit.  A Replacement kit(s), containing the patient’s assigned trial drug, will 
also be supplied at each visit, as needed during the study.  The Replacement kit(s) are to be 
returned at each study visit so that drug accountability can be performed.  The Replacement kits 
are to be used in situations where a visit is outside the visit window and the patient required 
additional trial drug prior to a visit and/or trial drug is inadvertently lost/damaged/destroyed.  
Parents/caregivers will bring the used and unused sachets, in the appropriate study kit box 
(normal study supply kit and the Replacement kit), to the site at each study visit.  The site will 
perform drug accountability (if all sachets were completely or partially utilized) at each study 
visit and record compliance for the previous visit. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
61 
10. 
TRIAL DRUG MATERIALS AND MANAGEMENT 
10.1. 
Trial Drug 
ZYN002 Transdermal Synthetic Cannabidiol Gel is a clear transdermal gel containing 4.2 % 
CBD for topical application.  
Placebo is an identical clear transdermal gel without the CBD.  
10.2. 
Trial Drug Packaging and Labeling 
ZYN002 drug product and placebo will be packaged in sachets. 
Study supplies will be labeled with a computer-generated label, which will include the following 
information:  
• Protocol Number  
• Intended Use 
• Storage Conditions 
• Labeled:  Keep out of reach of children 
• Identification Manufacturer/Sponsor 
10.3. 
Trial Drug Storage 
Trial drug and placebo are to be stored between 15°C - 25°C / 59°F - 77°F. 
10.4. 
Trial Drug Preparation 
Trial drug or placebo will be applied directly from the sachets as received. 
10.5. 
Administration 
At Visit 2 Placebo Lead-In, parents/caregivers will be instructed on proper application of the 
study gel (active/placebo gel).  
 
 Parents/caregivers will apply all trial drug to clean, dry, 
intact skin, thoroughly massaging it into the right and/or left upper arms and shoulders until the 
area is dry.  The study gel will be rubbed in completely and the skin must be dry prior to 
dressing. 
 
  The application site should 
be covered to minimize sun exposure when going outside during the day.  Parents/caregivers will 
use gloves supplied by the Sponsor to apply the study gel.  If redness occurs at the application 
site, the parent (after consultation with the Investigator) may switch the application temporarily 
to the upper thighs.  
 
 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
62 
10.6. 
Trial Drug Accountability 
Parents/caregivers will bring the used sachets in a plastic bag, provided by the site, at each study 
visit.  Any unused sachets will be returned in their box at each study visit to the clinic.  The site 
will perform drug accountability (if all sachets were completely or partially utilized) at each 
study visit and record trial drug compliance since the previous visit.  The number of used and 
unused sachets returned by the parent/caregiver will be counted and recorded on the appropriate 
CRF page. 
10.7. 
Trial Drug Handling and Disposal 
The site will place all used returned sachets in the plastic bag in the kit box labeled with the 
appropriate patient information.  For drug accountability purposes the patient number, initials 
and Visit number will be written on the outside of the study kit box. 
The study monitor will confirm the number of unused sachets of trial drug with the research 
facility and coordinate return or disposal of the used and unused supplies. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
63 
11. 
ASSESSMENT OF EFFICACY 
Efficacy assessments are as indicated in Table 3 Schedule of Assessments.  
11.1. 
Primary Endpoint 
The primary endpoint is change from Baseline to Week 12 in the ABC-CFXS Social Avoidance 
subscale score.  
11.2. 
Key Secondary Endpoints 
a) Change from Baseline to Week 12 in ABC-CFXS Irritability subscale score. 
b) Change from Baseline to Week 12 in ABC-CFXS Socially Unresponsive/Lethargic 
subscale score.  
c) CGI-I at Week 12/ET. 
11.3. 
Secondary Endpoints 
a) Percent of patients who have ≥ 25% improvement from Baseline in ABC-CFXS Social 
Avoidance subscale score at Week 12/ET. 
b) Percent of patients who have ≥ 25% improvement from Baseline in ABC-CFXS Irritability 
subscale score at Week 12/ET. 
c) Change from Baseline in ABC-CFXS Social Avoidance, Irritability, and Socially 
Unresponsive/Lethargic subscale scores at Weeks 4 and 8. 
d) Change from Baseline in ABC-CFXS Stereotypy, Inappropriate Speech, and Hyperactivity 
subscale scores at Weeks 4, 8, and 12.  
e) Change from Baseline to Weeks 4, 8, and 12 in ADAMS total score and subscale scores 
(Manic/Hyperactive Behavior, Depressed Mood, Social Avoidance, General Anxiety and 
Compulsive Behavior).  
f) CGI-I at Weeks 4 and 8. 
g) Percent of patients indicating improvement on the CGI-I (dichotomized) scale at Weeks 
4, 8, and 12/ET. 
h) Response rate for patients having both ≥ 25% improvement from Baseline in ABC-CFXS 
Social Avoidance subscale score AND improved on CGI-I scale at Weeks 4, 8, and 
12/ET. 
i) Response rate for patients having both ≥ 25% improvement from Baseline in ABC-CFXS 
Irritability subscale score AND improved on CGI-I scale at Weeks 4, 8, and 12/ET 
j) Change from Baseline in CGI-S at Weeks 4, 8, and 12/ET. 
k) Change from Baseline in Family Impact PedsQLTM at Week 12/ET. 
 
 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
64 
11.4. 
Method of Treatment Assignment or Randomization 
Patients will be randomly assigned to treatment according to a computer generated 
randomization scheme.  Randomization will be coordinated centrally through an interactive 
response system (IRT).  The system will provide patient identification numbers at Screening, 
which are subsequently linked to the treatment assignments at randomization.  Following 
completion of all evaluations at Visit 3, patients who meet all eligibility requirements and 
randomization criteria will be randomly allocated to active or placebo treatment groups using a 
1:1 allocation ratio.  Randomization will be stratified by gender (Male vs. Female), weight (≤ 35 
kg vs. > 35 kg) and region (North America vs. non-North America). 
11.5. 
Breaking the Blind  
This is a double-blind study.  During the conduct of the study, the patient, Investigator and study 
personnel at each center, and the Sponsor or its designated personnel directly involved in the 
clinical study will remain blinded to study treatment.  The Investigator will not be provided with 
the randomization scheme.  The randomization scheme will be maintained within the IRT.  The 
Investigator should contact the Medical Monitor to discuss individual situations prior to breaking 
the blind.   
 
If a medical emergency occurs and a decision regarding the patient's condition/treatment requires 
knowledge of the treatment assignment, the study blind may be broken for the specific patient 
via the IRT; the Investigator will immediately notify the Medical Monitor of the situation.  The 
date, time, and reason for un-blinding must be documented in the source document and in the 
appropriate section of the CRF.  Additionally, the documentation received from the IRT 
indicating the blind break must be retained in a secure manner, in the patient’s source 
documents.     

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
65 
12. 
ASSESSMENT OF SAFETY 
12.1. 
Safety Parameters 
Safety assessments will include collection of AEs including seizure assessment if applicable, 
physical and neurological examination, Tanner stage assessment, 12-lead ECG, clinical 
laboratory assessments (hematology, chemistry, and urinalysis), vital signs, C-SSRS, MWC, 
PWC-20, and findings from the skin irritation checks following treatment.  MADDERS will be 
used to systemically capture and adjudicate abuse-related events.  All patients who receive at 
least one dose of randomized trial drug will be included in the safety analysis.   
At the discretion of the investigator, patients will be allowed a short acting sedative (such as 
midazolam) to assist with the collection of blood samples and ECG’s. 
12.2. 
Adverse and Serious Adverse Events 
Throughout the study, the Investigator will monitor each patient for evidence of drug intolerance 
and for the development of clinical and/or laboratory evidence of an AE.  An AE assessment will 
be made by the Investigator on a routine basis at each study visit.  In order to standardize the 
approach to assessing the occurrence of AEs, the Investigator should make a judgement as to any 
change in condition or AEs that were not present before trial drug administration when he 
obtains the patient’s response to how they are feeling.  Patients having AEs will be followed 
until they return to normal or become stabilized. 
Investigators and study staff will be trained to recognize abuse, misuse and addiction.  They will 
be instructed to document all cases in which study drug is taken in a manner that deviates from 
the protocol, is unaccounted for, or is used by anyone other than the study participant.  In 
addition, MADDERS® will be used to systematically capture and adjudicate abuse-related events 
in this study (Treister et al. 2016). 
All AEs that occur during the course of the study (starting at the time the informed consent is 
signed) must be reported in detail on the appropriate CRFs and patient’s source document record 
and on any other report form required by national law.  All efforts will be made to follow-up 
events until resolution. 
12.2.1. 
Definition of Adverse Events 
12.2.1.1. Adverse Event (AE) 
An AE is an undesirable medical occurrence (sign, symptom, or diagnosis) or worsening of a 
pre-existing medical condition (e.g., diabetes, congestive heart failure, rheumatoid arthritis, 
psoriasis) that occurs at any time after signing of the ICF whether or not it is considered to be 
related to treatment.  Worsening of an existing medical condition is when a condition present at 
the time of signing of the ICF (e.g., cancer, diabetes, gout) becomes more severe, more frequent, 
or increased in duration during the study.  Hospitalizations for pretreatment conditions (e.g., 
elective cosmetic procedures) or surgeries that were planned before entry into the study are not 
considered AEs.  AEs will be collected from the signing of the ICF through the 4th week of the 
weekly follow-up phone call. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
66 
The parents/caregivers of patients with a current epilepsy and/or seizure diagnosis will be asked 
at each study visit if there has been any increase in frequency or severity of the patient’s seizures.  
If an increase in frequency or severity is noted, this will be recorded as an AE in the CRF. 
The Investigator is responsible for reviewing laboratory test results and determining whether an 
abnormal value represents for the patient a change from the time of signing of the ICF.  In 
general, abnormal laboratory findings without clinical significance (based on the Investigator's 
judgement) should not be recorded as AEs.  Clinically significant changes occurring after the 
signing of the ICF are considered AEs; however, the reported adverse event should include the 
underlying diagnosis or resulting clinical sequelae.  Patients having clinically significant AEs 
will be followed until they return to normal or become stabilized. 
12.2.1.1.1. Application site irritation scores  
 
 
 
  
 
 
 
 
 
 
12.2.1.1.2. Pregnancy 
If a patient or partner of a patient becomes pregnant during or after exposure to the trial drug 
(within three months of trial drug discontinuation) received in this study, the Investigator will 
immediately discontinue the patient from the study (if patient is still on study) and contact the 
Sponsor or designee.  The Investigator will complete the Sponsor’s (or designee’s) Clinical 
Pregnancy Notification Form and alert the Sponsor within two days of learning of the pregnancy.  
Diligent efforts will be made to determine the outcome for all pregnancy exposures in the 
clinical trial.  Information on the status of the mother and the child will be forwarded to the 
Sponsor. 
Generally, follow-up will occur within six to eight weeks following the estimated delivery date.  
Any premature termination of the pregnancy will be reported.  Both maternal and paternal 
exposure will be collected.  For exposure involving the female partner of a male patient, the 
necessary information must be collected from the patient, while respecting the confidentiality of 
the partner. 
Pregnancy itself is not regarded as an AE unless there is a suspicion that an investigational 
product may have interfered with the effectiveness of a contraceptive medication. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
67 
The outcome of all pregnancies (spontaneous miscarriage, elective termination, normal birth or 
congenital abnormality) must be followed up and documented even though the patient was 
discontinued from the study. 
All reports of congenital abnormalities/birth defects are SAEs.  Spontaneous miscarriages should 
also be reported and handled as SAEs.  Elective abortions without complications should not be 
handled as AEs.  The date of onset of the adverse event will be collected.  Also, the date of the 
resolution of the adverse event will be collected. 
12.2.1.2. Serious Adverse Event (SAE) 
Any adverse event that results in one or more of the following is considered an SAE. 
1. Death 
2. Life Threatening - The patient was at risk of death at the time of the event.  It does not 
refer to the hypothetical risk of death if the adverse event were more severe or were to 
progress 
3. In-patient hospitalization (admission or prolongation of existing hospitalization).  
Planned hospitalizations to treat a pre-existing condition or an inpatient stay required by 
the protocol is not considered to be an SAE unless otherwise specified in the study 
protocol.  If there is any doubt whether the hospitalization constitutes an SAE, it should 
be treated as serious and reported to the Sponsor. 
4. Persistent or significant disability / incapacity - Any AE having an outcome that is 
associated with a substantial disruption of the ability to carry out normal life functions.  
This includes the inability to work.  This is not intended to include transient interruptions 
of daily activities. 
5. Congenital abnormality or birth defect - Any structural abnormality in patient offspring 
that occurs after intrauterine exposure to treatment. 
6. Other Medically Important Events - Important medical events that may not result in 
death, be life-threatening, or require hospitalization may be considered a serious adverse 
event when, based upon medical judgement, they may jeopardize the patient or may 
require intervention to prevent one of the outcomes listed above. 
12.2.1.3. Other Adverse Event (OAE) 
OAEs will be identified by the study Medical Monitor during the evaluation of safety data for the 
Clinical Study Report.  Significant adverse events of particular clinical importance, other than 
SAEs and those AEs leading to discontinuation of the patient from the study, will be classified as 
OAEs.  For each OAE, a narrative may be written and included in the Clinical Study Report. 
12.3. 
Recording Adverse Events 
Adverse events spontaneously reported by the patient/parent/caregiver and/or in response to an 
open question from the study personnel or revealed by observation will be recorded during the 
study at the investigational site.  Clinically significant changes as judged by the Investigator in 
laboratory values, blood pressure, and pulse will be reported as AEs.  Abnormal values that 
constitute an SAE or lead to discontinuation of administration of trial drug must be reported and 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
68 
recorded as an AE/SAE.  Information about AEs/SAEs will be collected from the signing of the 
ICF until the last Telephone Follow-Up call.  The AE term should be reported in standard 
medical terminology when possible.   
For each AE, the Investigator will evaluate and report the onset date, resolution date, intensity, 
causality, action taken, serious outcome (if applicable), and whether or not it caused the patient 
to discontinue the study. 
The relationship of the adverse event to the trial drug will be assessed using the following 
definitions: 
• Related (An adverse event has a strong temporal relationship to trial drug or recurs on re-
challenge, and another etiology is unlikely).  
• Not Related (An adverse event is due to underlying or concurrent illness or effect of 
another drug and is not related to the trial drug (e.g., has no temporal relationship to trial 
drug or has a much more likely alternative etiology).  The alternative etiology will be 
recorded in the CRF. 
Intensity will be assessed according to the following scale: 
• Mild (awareness of sign or symptom, but easily tolerated) 
• Moderate (discomfort sufficient to cause interference with normal activities) 
• Severe (incapacitating, with inability to perform normal activities) 
It is important to distinguish between serious and severe AEs.  Severity is a measure of intensity 
whereas seriousness is defined by the criteria under Section 12.2.1.2.  An AE of severe intensity 
may not be considered serious. 
Should a pregnancy occur, it must be reported and recorded on the Sponsor’s (or designee’s) 
pregnancy form.  Pregnancy itself is not regarded as an AE unless there is a suspicion that an 
investigational product may have interfered with the effectiveness of a contraceptive medication. 
The outcome of all pregnancies (spontaneous miscarriage, elective termination, normal birth or 
congenital abnormality) must be followed up and documented even though the patient was 
discontinued from the study. 
All reports of congenital abnormalities/birth defects are SAEs.  Spontaneous miscarriages should 
also be reported and handled as SAEs.  Elective abortions without complications should not be 
handled as AEs. 
12.4. 
Reporting Adverse Events 
If any protocol defined expedited event, or serious AE occurs whether related to trial drug 
or not, the Investigator must notify the Sponsor within 24 hours by telephone or email. 
24 Hour SAE Telephone:  
 
Email:  
 
All SAEs (related and unrelated) will be recorded from the signing of the ICF until the last 
Telephone Follow-Up call.  Any SAEs considered related to the investigational product and 
discovered by the Investigator at any time after the study should be reported.  All SAEs must be 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
69 
reported to Zynerba within one business day of the first awareness of the event.  The Investigator 
must complete, sign and date the SAE form, verify the accuracy of the information recorded on 
the SAE form with the corresponding source documents, and send a copy by e-mail to Zynerba.   
Additional follow-up information, if required or available, should all be e-mailed to Zynerba 
within one business day of receipt and this should be completed on a follow-up SAE form and 
placed with the original SAE information and kept with the appropriate section of the CRF 
and/or study file. 
Zynerba is responsible for notifying the relevant regulatory authorities of certain events.  It is the 
Investigator’s responsibility to notify the Institutional Review Board (IRB) or Independent 
Ethics Committee (IEC) of all SAEs that occur at his or her site.  Investigators will also be 
notified of all unexpected, serious, drug-related events (7/15 Day Safety Reports) that occur 
during the clinical trial.  Each site is responsible for notifying its IRB or IEC of these additional 
SAEs. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
70 
13. 
ASSESSMENT OF PHARMACOKINETICS 
13.1.1. 
Blood Levels of AED, CBD and THC 
13.1.1.1. Blood Sample Collection 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
  Plasma samples will be shipped to 
the central laboratory.  The samples will be stored until the central laboratory batch ships them to 
the analytical laboratory chosen by the Sponsor. 
An inventory of the samples shipped will accompany the package. 
13.1.1.2. Sample Analysis 
Plasma samples will be analyzed
 
 
 
Plasma samples for adjunctive AEDs will be analyzed through a commercial laboratory.   
All analyses will be completed to GLP standards.  Results will be provided in a separate 
bioanalytical report. 
 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
71 
14. 
STATISTICS 
A Statistical Analysis Plan (SAP) will be prepared after the protocol is approved.  This document 
will provide further details regarding the definition of analysis variables and analysis 
methodology to address all study objectives. 
14.1. 
Sample Size Determination 
The sample size was estimated for the primary efficacy endpoint using Power Analysis and 
Sample Size Software (PASS 15) (NCSS, 2017).  Sample size requirements were based on a 
previous Phase 1/2 trial, ZYN2-CL-009, in patients with FXS and on published results in the 
literature with other studied treatments in this indication.  
 
 
 
 
 
14.2. 
Analysis Populations 
The safety analysis set will include all enrolled patients who receive at least one dose of trial 
drug (which includes placebo run-in). 
The randomized safety analysis set will include all patients who receive at least one dose of 
randomized trial drug.  The randomized safety analysis will be the primary population for safety 
analyses.  The treatment group assignment for this population will be the treatment actually 
received.    
The full analysis set (FAS) will include all patients in the randomized safety analysis set who 
have both a baseline and a post-baseline ABC-CFXS assessment.  The FAS set will serve as the 
primary efficacy study population.  The treatment group assignment for analysis will be the 
treatment the patient was randomized to.  
The PK population will include all patients who have at least one sample collected during 
treatment and analyzed for determination of the CBD and THC concentrations.  
14.3. 
Efficacy Analysis 
Descriptive statistics (mean, median, standard deviation, minimum, and maximum) for 
continuous data and number (n) and percentage (%) for categorical data will be presented for all 
efficacy and safety parameters.  
All efficacy assessments will be summarized by randomized treatment group.  
14.3.1. 
Primary Efficacy Analyses 
Null Hypothesis: 
The null-hypothesis to be tested is that there is no difference in effect between the active 
treatment group and placebo in the change from Baseline to Week 12 in the ABC-CFXS Social 
Avoidance subscale. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
72 
The change from Baseline in the ABC-C FXS Social Avoidance subscale will be analyzed using 
MMRM.  The MMRM will contain fixed effects for treatment, visit, study site, region and 
treatment-by-visit interaction as factors as well as the continuous fixed covariate of Baseline 
value for the dependent variable of interest.  For the MMRM model, descriptive statistics will be 
presented by visit, which will include least-squares mean, least-squares standard errors of the 
mean, least-squares meant treatment difference (active-placebo), 95% confidence intervals 
around the least-squares (LS) mean treatment difference and p-values for between treatment 
group tests.  MMRM will model the data specifying an unstructured variance-covariance 
structure.  Details will be presented in the SAP for situations where convergence is not met using 
the unstructured variance-covariance structure.   
14.3.2. 
Key Secondary Efficacy Analyses 
The key secondary endpoint, change from Baseline to Week 12 in the ABC-CFXS Irritability, and 
ABC-CFXS Socially Unresponsive/Lethargic subscale score, will be analyzed using the same 
MMRM approach as the primary endpoint.   
 
The CGI-I at Week 12/ET will be analyzed using the Wilcoxon rank sum test.   
 
The response rate for the percent of patients who have ≥ 25% improvement at Week 12/ET from 
Baseline in the ABC-CFXS Irritability, and ABC-CFXS Social Avoidance subscale score will be 
compared using the chi-square test and binomial confidence intervals. 
14.3.3. 
Secondary Efficacy Analyses 
All secondary endpoints including change from Baseline in ABC-CFXS Social Avoidance, 
Irritability, Inappropriate Speech, Stereotypy and Hyperactivity subscales scores and changes 
from Baseline in the ADAMS total score and subscale scores, will also be analyzed using the 
same MMRM approach as the primary endpoint.   
The statistical comparison of active versus placebo for the CGI-I at weeks 4 and 8 will be based 
on Wilcoxon rank sum test.  The statistical comparison of active versus placebo for the CGI-S 
will be based on a covariate adjusted Cochran-Mantel-Haenszel (CMH) test, adjusting for the 
baseline score as the covariate.   
A patient will be considered as “improved” on the CGI-I scale if they are rated as “1-very much 
improved” or “2-much improved”.  All other ratings, including missing values, will be 
considered “not improved” (Guy 1976; Berry-Kravis et al. 2017).  The response rates for the 
percent of patients improved using CGI-I , and for patients having both ≥ 25% improvement 
from Baseline in ABC- CFXS Social Avoidance subscale score AND improved on CGI-I scale 
will be compared using the chi-square test and binomial confidence intervals.   
Change from Baseline in Family Impact PedsQLTM will be analyzed using ANCOVA.  
ANCOVA models will contain fixed effects for treatment, region and study site with Baseline 
value for the dependent variable of interest as a covariate. 
For MMRM/ANCOVA, descriptive statistics will be presented by visit, which will include LS 
mean, LS standard errors, LS mean treatment difference (active-placebo) and associated 95% 
confidence interval (CI) and p-values for between treatment group tests.  

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
73 
14.3.4. 
Exploratory Analysis 
Results of exploratory analysis of the Caregiver Global Impression of Severity, the Caregiver 
Global Impression of Change, and the Qualitative Caregiver reported Behavioral Survey will be 
reported using descriptive statistics. 
14.4. 
Safety Analysis 
AEs will be tabulated by the actual treatment dose of trial drug received at the time of initiation 
of the adverse event and classified by system organ class and preferred term using MedDRA.  
Additionally, AEs will be tabulated overall (total number of AEs and total number of patients 
with AEs).  
MADDERS® will be used in this study to systematically capture and adjudicate abuse-related 
events. (Treister et al. 2016).  Data from the MADDERS® will be summarize and presented 
separately from other AEs.   
Symptoms of withdrawal will also be assessed by comparing change over time in the total score 
on the MWC and total score on the PWC-20 from Baseline to Week 12/ET, weekly during the 
taper period and weekly for four weeks after the patient stops study drug. 
Vital signs assessments (actual and change from Baseline) will be summarized using descriptive 
statistics and presented by actual treatment group.  
Safety laboratory and urinalysis assessments (actual and change from Baseline) will be 
summarized by actual treatment group. 
ECGs (actual and change from Baseline) will be summarized by actual treatment group.  ECG 
results including any clinically significant findings will be summarized at each study visit. 
Application site irritation check will be summarized using counts and percentages at each 
respective site check score (‘0’, ‘1’, ‘2’, ‘3’, or ‘4’) by actual treatment group. 
14.5. 
Pharmacokinetic Analysis 
Plasma concentrations for CBD, THC and concurrent AEDs will be summarized using 
descriptive statistics and presented by randomized treatment group (active or placebo) and by 
Screening and Treatment time points.  
 
 
 
 
 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
74 
14.6. 
Interim Analysis 
There is no planned interim analysis for this study. 
14.7. 
Adjustments for Multiplicity 
 
 
 
  
 
 
 
 
 
 
 
 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
75 
15. 
DIRECT ACCESS TO SOURCE DATA/DOCUMENTS 
15.1. 
Study Monitoring 
Before an investigational site can enter a patient into the study, a representative of Zynerba will 
visit the investigational study site to: 
• Determine the adequacy of the facilities 
• Discuss with the Investigator(s) and other personnel their responsibilities with regard to 
protocol adherence, and the responsibilities of Zynerba or its representatives.  This will 
be documented in a Clinical Study Agreement between Zynerba and the Investigator. 
During the study, a monitor from Zynerba or representative will have regular contact with the 
investigational site, for the following: 
• Provide information and support to the Investigator(s). 
• Confirm that facilities remain acceptable. 
• Confirm that the investigational team is adhering to the protocol, data are being 
accurately recorded in the case report forms, and that investigational product 
accountability checks are being performed. 
• Perform source data verification.  This includes a comparison of the data in the case 
report forms with the patient’s medical records at the hospital or practice, and other 
records relevant to the study.  This will require direct access to all original records for 
each patient (e.g. clinic charts). 
• Record and report any protocol deviations not previously sent to Zynerba. 
• Confirm AEs and SAEs have been properly documented on CRFs and confirm any SAEs 
have been forwarded to Zynerba and those SAEs that met criteria for reporting have been 
forwarded to the IRB/IEC. 
The monitor will be available between visits if the Investigator(s) or other staff needs 
information or advice. 
15.2. 
Audits and Inspections 
Authorized representatives of Zynerba, a regulatory authority, an Independent Ethics Committee 
or an Institutional Review Board may visit the site to perform audits or inspections, including 
source data verification.  The purpose of a Zynerba audit or inspection is to systematically and 
independently examine all study-related activities and documents to determine whether these 
activities were conducted, and data were recorded, analyzed, and accurately reported according 
to the protocol, Good Clinical Practice (GCP) guidelines of the International Conference on 
Harmonization (ICH), and any applicable regulatory requirements.  The Investigator should 
contact Zynerba immediately if contacted by a regulatory agency about an inspection. 
 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
76 
15.3. 
Institutional Review Board (IRB)/Independent Ethics Committee 
(IEC) 
The Investigator must obtain IRB/IEC approval for the investigation.  Initial IRB/IEC approval, 
and all materials approved by the IRB/IEC for this study including the patient consent form and 
recruitment materials must be maintained by the Investigator and made available for inspection. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
77 
16. 
QUALITY CONTROL AND QUALITY ASSURANCE 
Original patient records such as research facility records and laboratory reports should be 
available at each site for source document review by Sponsor personnel.  Source document 
review is the verification of the information recorded on CRFs with that recorded in the original 
patient records.  In this study, source document review of specific types of information will be 
conducted for all patients. 
To ensure compliance with Good Clinical Practices and all applicable regulatory requirements, 
Zynerba, or its representative, may conduct a quality assurance audit.  Please see Section 18.1 for 
more details regarding the audit process. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
78 
17. 
ETHICS 
17.1. 
Independent Ethics Committee or Institutional Review Board 
GCP requires that the clinical protocol, any protocol amendments, the Investigator’s Brochure, 
the ICF, and all other forms of patient information related to the study (e.g., advertisements used 
to recruit patients) and any other necessary documents be reviewed by an IEC/IRB.  IEC/IRB 
approval of the protocol, ICF and patient information and/or advertising, as relevant, will be 
obtained prior to the authorization of drug shipment to a study site.  Any amendments to the 
protocol will require IEC/IRB approval prior to implementation of any changes made to the 
study design. 
17.2. 
Ethical Conduct of the Study 
The study will be conducted in accordance with the Declaration of Helsinki and GCP guidelines.  
Each Investigator will complete the Statement of Investigator form (FDA 1572 form).  The 
Investigator is responsible for reporting to the IEC/IRB modifications, safety updates, 
amendments, and deviations of the protocol that impact on patient safety. 
At appropriate intervals, the clinical monitor will visit the site during the clinical study and 
assure that the Investigator’s obligations are being fulfilled.  Per GCP requirement for 
confirmatory proof of patient files, a copy of all records must be retained with the files of the PI 
for a minimum of 15 years.  These records include the Confidential Follow-up Forms and other 
documents such as ICFs, laboratory reports, and other source documents, drug accountability 
forms, IEC/IRB approvals, protocols, and CRFs. 
17.3. 
Patient Information and Informed Consent 
The study protocol and ICF (and assent if applicable) must be approved by the Investigator’s 
IEC/IRB and a copy of the approved ICF (and assent if applicable) must be supplied to the 
Sponsor.  The parent/caregiver will be asked to read the consent form.  If the parent/caregiver 
decides that the patient should participate in the study, the parent/caregiver will be asked to sign 
and date the form as evidence of consent and as the Legalized Authorized Representative.  Each 
parent/caregiver must voluntarily sign and date a consent form before the patient participates in 
this study.  It is the obligation of the Investigator or their representative to explain the nature of 
the study to the parent/caregiver.  The Investigator will document in the patient’s medical chart 
that the parent/caregiver has signed an ICF to participate in an investigational trial, a copy of the 
ICF will be given to the parent/caregiver, and the original should be retained with the patient’s 
study records. 
Patient names will remain confidential.  Only the patient number, patient initials, and birth date 
will be recorded in the CRF.  The parent/caregiver will give explicit permission for 
representatives of the regulatory authorities and the IRB/IEC to inspect the patient’s medical 
records to verify the information collected.  Parents/caregivers will be informed that all protected 
health information and clinical data are saved in a confidential manner.  
All study data are confidential with restricted access.  Information made available for inspection 
will be handled in the strictest confidence and in accordance with all state, local, and federal data 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
79 
protection/privacy laws, including, without limitation, the Health Insurance Portability and 
Accountability Act (HIPAA). 
All parents/caregivers of patients in the United States will provide written authorization to 
disclose protected health information either as a part of the written ICF or as a separate 
authorization form.  The authorization will contain all required elements specified by the FDA 
45 Code of Federal Regulations (CFR) 164.  The parent/caregiver will be informed that the 
authorization does not expire.  The parent/caregiver will be informed they can revoke this 
authorization at any time by giving written notice.  If the parent/caregiver revokes authorization 
the patient will not be permitted to continue in the study.  The revoking of authorization cannot 
be considered retroactive to data already collected under an existing authorization.  Individual 
patient medical information obtained during this study is confidential and its disclosure to third 
parties, other than those mentioned in this section, is strictly prohibited.  In addition, medical 
information obtained during this study may be provided to the patient’s personal physician or to 
other appropriate medical personnel when required in connection with the patient’s continued 
health and welfare. 
The Investigator will maintain a personal patient identification list (patient and enrollment 
numbers with the corresponding patient names) to enable records to be identified. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
80 
18. 
DATA HANDLING AND RECORDKEEPING 
18.1. 
Inspection of Records 
The Sponsor will be allowed to conduct site visits to the investigation facilities for the purpose of 
monitoring any aspect of the study.  The Investigator agrees to allow the monitor to inspect the 
drug storage area, trial drug stocks, drug accountability records, patient charts and study source 
documents, and other records relative to study conduct. 
Source documents are defined as original documents, data and records.  This may include 
hospital records, clinical and office charts, laboratory data/information, evaluation checklists, 
pharmacy dispensing and other records, recorded data from automated instruments, microfiches, 
photographic negatives, microfilm or magnetic media, and/or x-rays. 
In addition, the Investigator will permit trial-related audits, IEC/IRB review, and regulatory 
inspection(s), providing direct access to source data documents. 
18.2. 
Retention of Records 
The Investigator must maintain all documentation relating to the study for a period of 15 years 
from study completion.  If it becomes necessary for the Sponsor or the Regulatory Authority to 
review any documentation relating to the study, the Investigator must permit access to such 
records. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
81 
19. 
PROTOCOL MODIFICATIONS IMPLEMENTED DURING THE 
COVID-19 PANDEMIC 
Due to the pandemic of Coronavirus (COVID-19), adjustments to the ZYN2-CL-016 protocol 
have been implemented, where necessary, and only at the time of the pandemic.  These changes 
may remain in place until such time that restrictions for travel and attendance at study visits are 
lifted.  The specific modifications are described below.   
19.1. 
Changes to Allow for Remote Patient Visits 
During the COVID-19 pandemic, when patient and parent/caregiver travel is restricted and/or 
investigative sites have restricted patient visits, remote patient study visits will be permitted.  
Zynerba has developed patient packets for the sites, corresponding to each remote visit, that will 
allow for many (but not all) of the study procedures to be performed remotely by trained site 
staff.  Those assessments that can be performed remotely include ABC-CFXS, ADAMS, CGI-S, 
CGI-I, Caregiver Global Impression of Change, Caregiver Global Impression of Severity, Family 
Impact Peds QLTM, C-SSRS, MWC, PWC-20, skin assessment exam (if using Skype, Facetime, 
etc.), daily skin diary, check on concomitant medications and check on adverse events.  As with 
onsite visits, the clinician or site staff conducting the remote visit will read the caregiver the 
rating instructions before ratings are completed.  The site will witness the parent/caregiver 
completing the assessments when video visits are conducted.  The parent/caregiver will return 
the completed assessments, along with the skin irritation diary, to the investigational site, using 
pre-printed shipping materials.  The site will then upload the completed questionnaires to the 
electronic system.  Safety assessments will all be conducted with the parent/caregiver on the 
phone or via video call. 
19.2. 
Changes to Allow for Investigational Product (IP) Shipment from 
Investigative Site to Parent/Legal Guardian 
 
 
  
 
  
 
 
 
 
    
 
 
 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
82 
 
 
 
 
19.3. 
Changes to Allow for Courier Return of Investigational Product 
from Parent/Legal Guardian to an Approved Depot 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
19.4. 
Changes to Allow Patients to Attend Local Laboratory Facilities to 
Obtain Blood Collection for Safety Laboratory Analyses 
To help ensure patient safety during the COVID-19 pandemic when onsite visits cannot be 
conducted, Zynerba has arranged for trial participants to be seen at local laboratories when a 
study visit requires a blood collection for safety laboratories.  Each of these local centers will 
have a work specifications worksheet, which describes the analyses to be completed and the 
process to follow for the Zynerba clinical trial participants.  
The Principal Investigator will complete a requisition for the patient to visit a local facility for 
sample collection.  The local laboratory facility will collect the blood sample, analyze, and report 
results to the Principal Investigator.  Site staff will be able to recommend the facility in closest 
proximity to the patient.   
19.5. 
Shifting of Some Safety Assessments to the Next Onsite Visit 
Since some of the patient visits will be completed remotely, several of the assessments normally 
done at an in-person visit will be shifted to the next onsite visit that a patient can attend.  These 
include ECG; targeted physical/neurological examinations, including Tanner stage assessments, 
if applicable; and vital signs.  Although the Principal Investigator will be able to check in on the 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
83 
well-being of the patient at remote visits, and assess for any new/increasing adverse events, the 
ECG, vital signs and targeted physical and neurological examinations will be deferred until the 
next visit on site.  Although local laboratories can be used for safety assessments, if a patient 
cannot travel to the closest local laboratory facility, the safety labs will be deferred to the next 
study visit. 
 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
84 
20. 
PUBLICATION POLICY 
All information concerning ZYN002 and the Sponsor’s operations, such as ZYN002 patent 
applications, formulas, manufacturing processes, basic scientific data, or formulation 
information, supplied by the Sponsor and not previously published, is considered confidential 
information. 
The information developed during the conduct of this clinical study is also considered 
confidential and will be used by the Sponsor in connection with the development of ZYN002.  
This information may be disclosed as deemed necessary by the Sponsor.  To allow for the use of 
the information derived from this clinical study and to ensure complete and thorough analysis, 
the Investigator is obligated to provide the Sponsor with complete test results and all data 
developed in this study. 
This confidential information shall remain the sole property of the Sponsor, shall not be 
disclosed to others without the written consent of the Sponsor, and shall not be used except in the 
performance of this study. 
Notwithstanding the foregoing, the rights, duties and obligations of Sponsor, PI and other 
Investigators involved in the study concerning the publication of the methods, results of, and 
conclusions from the study shall be subject to the applicable provisions of the clinical trial 
research agreement entered into between Sponsor and as applicable, the institutions, study sites 
and/or Investigators who undertake to perform the study.  At this time, Sponsor has not 
definitively determined whether it will publish study results.  However, under the terms of the 
Australia Medicines Standard Clinical Trials Research Agreement (CTRA) (the “Standard 
CTRA”), the New Zealand Association of Clinical Research standardized Clinical Trial Research 
Agreement (“sCTRA”), and Sponsor’s template Clinical Trial Research Agreement for use with 
institutions, study sites and/or Investigators who undertake study activities in the United States 
(“Sponsor CTRA”), even if Sponsor determines not to publish study results, Investigators shall 
have the right to publish such results so long as they comply with terms of the Standard CTRA, 
sCTRA or Sponsor CTRA, as applicable. 
 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
85 
21. 
LIST OF REFERENCES 
American Psychiatric Association.  2013.  Diagnostic and statistical manual of mental 
disorders.5. Washington, DC. 
Bakas T, van Nieuwenhuijzen PS, et al.  2017.  The direct actions of cannabidiol and 2-
arachidonoyl glycerol at GABAA receptors.  Pharmacological Research.  119:358-370. 
Bebin ME, Comi AM, Patel AD, Joshi C, Checketts D, Beal JC, Laux LC, De Boer LM, Wong 
MH, Lopez M, et al.  2017.  Long-Term Efficacy and Safety of Cannabidiol (CBD) in Children 
and Adults with Treatment Resistant Epilepsies (TRE).  American Epilepsy Society Annual 
Meeting.  Washington, DC.  Abstract 2.427. 
Bergamaschi MM, Queiroz RH, Chagas MH, de Oliveira DC, De Martinis BS, Kapczinski F, 
Quevedo J, Roesler R, Schröder N, Nardi AE, Martín-Santos R, Hallak JE, Zuardi AW, Crippa 
JA.  2011b.  Cannabidiol reduces the anxiety induced by simulated public speaking in treatment-
naïve social phobia patients.  Neuropsychopharmacology.  36(6):1219-1226. 
Berry-Kravis E, Hagerman R, Visootsak J, Budimirovic D, Kaufmann WE, Cherubini M, et al.  
2017.  Arbaclofen in fragile x syndrome: Results of phase 3 trials.  Journal of 
Neurodevelopmental Disorders.  9:3. 
Bhattacharyya S, Crippa JA, Allen P, Martin-Santos R, Borgwardt S, Fusar-Poli P, Rubia K, 
Kambeitz J, O'Carroll C, Seal ML, Giampietro V, Brammer M, Zuardi AW, Atakan Z, McGuire 
PK.  2012.  Induction of psychosis by Δ9-tetrahydrocannabinol reflects modulation of prefrontal 
and striatal function during attentional salience processing.  Arch Gen Psychiatry.  69(1):27-36. 
Bhattacharyya S, Morrison PD, Fusar-Poli P, Martin-Santos R, Borgwardt S, Winton-Brown T, 
Nosarti C, O' Carroll CM, Seal M, Allen P, Mehta MA, Stone JM, Tunstall N, Giampietro V, 
Kapur S, Murray RM, Zuardi AW, Crippa JA, Atakan Z, McGuire PK.  2010.  Opposite effects 
of delta-9-tetrahydrocannabinol and cannabidiol on human brain function and psychopathology.  
Neuropsychopharmacology.  35(3):764-774. 
Bisogno T, Hanus L, De Petrocellis L, Tchilibon S, Ponde DE, Brandi I, Moriello AS, Davis JB, 
Mechoulam R, Di Marzo V.  2001.  Molecular targets for cannabidiol and its synthetic 
analogues: effect on vanilloid VR1 receptors and on the cellular uptake and enzymatic hydrolysis 
of anandamide.  Br J Pharmacol.  134(4):845-852. 
Blessing EM, Steenkamp MM, Manzanares J, Marmar CR.  2015.  Cannabidiol as a Potential 
Treatment for Anxiety Disorders.  Neurotherapeutics.  12(4):825-36. 
Borgwardt SJ, Allen P, Bhattacharyya S, Fusar-Poli P, Crippa JA, Seal ML, Fraccaro V, Atakan 
Z, Martin-Santos R, O'Carroll C, Rubia K, McGuire PK.  2008.  Neural basis of Delta-9-
tetrahydrocannabinol and cannabidiol: effects during response inhibition.  Biol Psychiatry.  
64(11):966-973. 
Budney AJ, Novy PL, Hughes JR.  1999.  Marijuana withdrawal among adults seeking treatment 
for marijuana dependence.  Addiction.  94(9):1311–1322. 
Budney AJ, Moore BA, Vandrey RG, Hughes JR.  2003.  The time course and significance of 
cannabis withdrawal.  Journal of abnormal psychology.  112(3):393. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
86 
Carlini EA, Cunha JM.  1981.  Hypnotic and antiepileptic effects of cannabidiol.  J Clin 
Pharmacol.  21(Suppl 8-9):417S-427S. 
Castillo PE, Younts TJ, Chávez AE, Hashimotodani Y.  2012.  Endocannabinoid signaling and 
synaptic function.  Neuron.  76(1):70-81. 
Chagas MH, Eckeli AL, Zuardi AW, Pena-Pereira MA, Sobreira-Neto MA, Sobreira ET, Camilo 
MR, Bergamaschi MM, Schenck CH, Hallak JE, Tumas V, Crippa JA. 2014.  Cannabidiol can 
improve complex sleep-related behaviours associated with rapid eye movement sleep behaviour 
disorder in Parkinson's disease patients: a case series.  Journal of Clinical Pharmacy and 
Therapeutics.  39:564-566. 
Consroe P, Laguna J, Allender J, Snider S, Stern L, Sandyk R, Kennedy K, Schram K.  1991.  
Controlled clinical trial of cannabidiol in Huntington's disease.  Pharmacol Biochem Behav.  
40(3):701-708. 
Crawford DC, Acuña JM, Sherman SL.  2001.  FMR1 and the fragile X syndrome: Human 
genome epidemiology review.  Genet Med.  3(5):359-371. 
Cunha JM, Carlini EA, Pereira AE, Ramos OL, Pimentel C, Gagliardi R, Sanvito WL, Lander N, 
Mechoulam R.  1980.  Chronic administration of cannabidiol to healthy volunteers and epileptic 
patients.  Pharmacology.  21(3):175-185. 
Dalterio SL and deRooij DG.  1986.  Maternal cannabinoid exposure Effects on spermatogenesis 
in male offspring.  International Journal of Andrology.  9:250-258. 
Dalterio S, Thomford PJ, et al.  1986.  Perinatal Cannabinoid Exposure: Effects on Hepatic 
Cytochrome P-450 and Plasma Protein Levels in Male Mice.  Teratology.  33:195-201. 
Dalterio S, Steger R, et al.  1984.  Early Cannabinoid Exposure Influences Neuroendocrine and 
Reproductive Functions in Male Mice: I. Prenatal Exposure.  Pharmacology Biochemistry & 
Behavior.  20:107-113. 
Dalterio S, Steger R, et al.  1984.  Early Cannabinoid Exposure Influences Neuroendocrine and 
Reproductive Functions in Mice: II. Postnatal Effects.  Pharmacology Biochemistry & Behavior.  
20:115-123. 
Devinsky O, Marsh E, Friedman D, Thiele E, Laux L, Sullivan J, et al.  2016.  Cannabidiol in 
patients with treatment-resistant epilepsy: an open-label interventional trial.  Lancet Neurol.  
15(3):270-8. 
Devinsky O, Cross, J, Laux, L, et al.  2017.  Trial of cannabidiol for drug-resistant seizures in the 
Dravet Syndrome.  N Eng J Med.  376:2011-20. 
Devinsky O, Patel AD, Thiele EA, Wong MH, Appleton R, Harden CL, Greenwood S, Morrison 
G, Sommerville K, et al.  2018.  Randomized, dose-ranging safety trial of cannabidiol in Dravet 
syndrome. Neurology.  90(14):e1204-e1211. 
Di Marzo V, Maccarrone M.  2008.  FAAH and anandamide: Is 2-AG really the odd one out?  
Trends Pharmacol Sci.  29(5):229-233. 
Elmes M, Kaczocha M, Berger W, Leung K, Ralph, B, Wang L, Sweeney J, Miyauchi J, Tsirka 
S, Ojima I, Deutsch D.  2015.  Fatty Acid-binding Proteins (FABPs) Are Intracellular Carriers 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
87 
for Delta 9-Tetrahydrocannabinol (THC) and Cannabidiol (CBD).  J Biological Chemistry.  
290(14):8711-8721. 
Englund A, Morrison PD, Nottage J, Hague D, Kane F, Bonaccorso S, Stone JM, Reichenberg 
A, Brenneisen R, Holt D, Feilding A, Walker L, Murray RM, Kapur S.  2013.  Cannabidiol 
inhibits THC-elicited paranoid symptoms and hippocampal-dependent memory impairment.  J 
Psychopharmacol.  27(1):19-27. 
Esbensen AJ, Rojahn J, Aman MG, Ruedrich S.  2003.  Reliability and Validity of an 
Assessment Instrument for Anxiety, Depression, and Mood among Individuals with Mental 
Retardation.  Journal of Autism and Developmental Disorders.  33(6). 
Filipovic-Sadic S, Sah S, Chen L, Krosting J, Sekinger E, Zhang W, Hagerman PJ, Stenzel TT, 
Hadd AG, Latham GJ, Tassone F.  2010.  A novel FMR1 PCR method for the routine detection 
of low abundance expanded alleles and full mutations in fragile X syndrome.  Clin Chem.  
56(3):399-408. 
Friedman D, Devinsky O.  2015.  Cannabinoids in the Treatment of Epilepsy.  N Engl J Med.  
373:1048-58. 
Fusar-Poli P, Allen P, Bhattacharyya S, Crippa JA, Mechelli A, Borgwardt S, Martin-Santos R, 
Seal ML, O'Carrol C, Atakan Z, Zuardi AW, McGuire P.  2010.  Modulation of effective 
connectivity during emotional processing by Delta 9-tetrahydrocannabinol and cannabidiol.  Int J 
Neuropsychopharmacol.  13(4):421-432. 
Fusar-Poli P, Crippa JA, Bhattacharyya S, Borgwardt SJ, Allen P, Martin-Santos R, Seal M, 
Surguladze SA, O'Carrol C, Atakan Z, Zuardi AW, McGuire PK.  2009.  Distinct effects of ∆ 9-
tetrahydrocannabinol and cannabidiol on neural activation during emotional processing.  Arch 
Gen Psychiatry.  66(1):95-105. 
Gaoni Y and Mechoulam R.  1966.  The Isomerization of Cannabidiol to Tetrahydrocannabinols.  
Tetrahedron.  22:1481-1488. 
Guy W.  1976.  Clinical Global Impression (CGI).  ECDEU Assessment Manual for 
Psychopharmacology. pp125-126. 
Guy GW, Flint ME.  2003.  A single centre, placebo-controlled, four period, cross-over, 
tolerability study assessing, pharmacodynamic effects, pharmacokinetic characteristics and 
cognitive profiles of a single dose of three formulations of cannabis based medicine extracts 
(CBMEs) (GWPD9901), plus a two period tolerability study comparing pharmacodynamic 
effects and pharmacokinetic characteristics of a single dose of a cannabis based medicine extract 
given via two administration routes (GWPD9901 EXT).  J Cannabis Ther.  3(3):35-77. 
Haldeman-Englert C.  2013.  Fragile X syndrome.  National Institutes of Health:  Medical 
Encyclopedia. 
Hallak JE, Dursun SM, Bosi DC, de Macedo LR, Machado-de-Sousa JP, Abrão J, Crippa JA, 
McGuire P, Krystal JH, Baker GB, Zuardi AW.  2011.  The interplay of cannabinoid and NMDA 
glutamate receptor systems in humans: preliminary evidence of interactive effects of cannabidiol 
and ketamine in healthy human subjects.  Prog Neuropsychopharmacol Biol Psychiatry.  
35(1):198-202. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
88 
Hare EB, Hagerman RJ, Lozano R.  2014.  Targeted treatments in fragile X syndrome.  Expert 
Opinion on Orphan Drugs.  2(6):1-13. 
Hazekamp A, Peltenburg A, et al.  2005.  Chromatographic and Spectroscopic Data of 
Cannabinoids from Cannabis Sativa L.  Journal of Liquid Chromatography & Related 
Technologies.  28(15):2361-2382. 
Hubert MF, Gillet JP, Durand-Cavagna G.  1994.  Spontaneous retinal changes in Spague 
Dawley rats. Lab Anim Sci.  44(6):561-567. 
Jung KM, Sepers M, Henstridge CM, Lassalle O, Neuhofer D, Martin H, Ginger M, Frick A, 
DiPatrizio NV, Mackie K, Katona I, Piomelli D, Manzoni OJ.  2012.  Uncoupling of the 
endocannabinoid signalling complex in a mouse model of fragile X syndrome.  Nat Commun.  
3:1080. 
Leigh MJ, Nguyen DV, Mu Y, Winarni TI, Schneider A, Chechi T, Polussa J, Doucet P, Tassone 
F, Rivera SM, Hessl D, Hagerman RJ.  2013.  A randomized double-blind, placebo-controlled 
trial of minocycline in children and adolescents with fragile x syndrome.  J Dev Behav Pediatr.  
34(3):147–155. 
Leweke FM, Piomelli D, Pahlisch F, Muhl D, Gerth CW, Hoyer C, Klosterkötter J, Hellmich M, 
Koethe D.  2012.  Cannabidiol enhances anandamide signaling and alleviates psychotic 
symptoms of schizophrenia.  Transl Psychiatry.  2:e94. 
Lord C, Risi S, Lambrecht L, Cook, Jr. EH, Leventhal BL, DiLavore PC, Pickles A, and Rutter 
M.  2000.  The Autism Diagnostic Observation Schedule - Generic: A Standard Measure of 
Social and Communication Deficits Associated with the Spectrum of Autism.  J Autism Dev 
Disord.  30(3):205-223. 
Lozano R, Martinez-Cerdeno V, Hagerman RJ.  2015.  Advances in the Understanding of the 
Gabaergic Neurobiology of FMR1 Expanded Alleles Leading to Targeted Treatments for Fragile 
X Spectrum Disorder.  Curr Pharm Des.  21(34):4927-4979. 
Martin-Santos R, Crippa JA, Batalla A, Bhattacharyya S, Atakan Z, Borgwardt S, Allen P, Seal 
M, Langohr K, Farré M, Zuardi AW, McGuire PK.  2012.  Acute effects of a single, oral dose of 
d9-tetrahydrocannabinol (THC) and cannabidiol (CBD) administration in healthy volunteers.  
Curr Pharm Des.  18(32):4966-4979. 
Matsuyama SS, Fu TK.  1981.  In vivo cytogenetic effects of cannabinoids.  J Clin 
Psychopharmacol.  1(3):135-140. 
Merrick J, Lane B, Sebree T.  2016.  Identification of Psychoactive Degradants of Cannabidiol in 
Simulated Gastric and Physiological Fluid.  Cannabis and Cannabinoid Research.  1.1. 
Mouslech Z, Valla V.  2009.  Endocannabinoid system: An overview of its potential in current 
medical practice.  Neuro Endocrinol Lett.  30(2):153-179. 
Ohno-Shosaku T, Kano M.  2014.  Endocannabinoid-mediated retrograde modulation of synaptic 
transmission.  Curr Opin Neurobiol.  29:1-8. 
Pacher P, Bátkai S, Kunos G.  2006.  The endocannabinoid system as an emerging target of 
pharmacotherapy.  Pharmacol Rev.  58(3):389-462. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
89 
PASS15 Power Analysis and Sample Size Software.  2017.  NCSS, LLC. Kaysville, Utah, USA. 
ncss.com/software/pass 
Pertwee RG.  2008.  The diverse CB1 and CB2 receptor pharmacology of three plant 
cannabinoids: Delta9-tetrahydrocannabinol, cannabidiol and delta9-tetrahydrocannabivarin.  Br J 
Pharmacol.  153(2):199-215. 
Perez-Reyes M, Timmons MC, Davis KH, Wall EM.  1973.  A comparison of the 
pharmacological activity in man of intravenously administered delta9-tetrahydrocannabinol, 
cannabinol, and cannabidiol.  Experientia.  29(11):1368-1369. 
Qin M, Zeidler Z, Moulton K, Krych L, Xia Z, Smith CB.  2015.  Endocannabinoid-mediated 
improvement on a test of aversive memory in a mouse model of fragile X syndrome.  Behav 
Brain Res. 291:164-171. 
Rohleder C, Müller JK, Lange B, Leweke FM.  Cannabidiol as a Potential New Type of an 
Antipsychotic.  A Critical Review of the Evidence.  Frontiers in Pharmacology.  7:422. 
Rickels K, Garcia-Espana F, Mandos LA, Case GW.  2008.  Physician Withdrawal Checklist 
(PWC-20). J Clin Psychopharmacol.  28(4):447-51.  
Sansone SM, Widaman KF, Hall SS, Reiss AL, Lightbody A, Kaufmann WE, Berry-Kravis E, 
Lachiewicz A, Brown EC, Hessl D.  2012.  Psychometric Study of the Aberrant Behavior 
Checklist in Fragile X Syndrome and Implications for Targeted Treatment.  J Autism Dev 
Disord. 42(7):1377–1392. 
Sparrow SS, Cicchetti DV, & Saulnier CA.  2016.  Vineland Adaptive Behavior Scales (3rd Ed.).  
Minneapolis, MN: Pearson Assessments, Inc. 
Stein L, Geddings B, Bartoe J, Nelson K.  2017.  Chorioretinal dysplasia in Sprague-Dawley 
rats:  ophthalmolgic and histopathologic correlation.  STP poster. 
Tanaka K, Inagaki S, Ohmori R, Kuno H, Matsumoto H, and Usui T.  1993.  Focal Chorioetinal 
Atrophy in Rats.  J Toxicol Pathol.  6:205-211. 
Tassone F, Pan R, Amiri K, Taylor AK, Hagerman PJ.  2008.  A rapid polymerase chain 
reaction-based screening method for identification of all expanded alleles of the fragile X 
(FMR1) gene in newborn and high-risk populations.  J Mol Diagn.  10(1):43-9.  
Tassone F, Hagerman RJ, Ikle DN, Dyer PN, Lampe M, Willemsen R, Oostra BA, Taylor AK.  
1999.  FMRP expression as a potential prognostic indicator in fragile X syndrome.  Am J Med 
Genet.  84(3):250-61. 
The Fragile X Clinical and Research Consortium.  2012.  Consensus of the Fragile X Clinical & 
Research Consortium on clinical practices:  Sleep in children with Fragile X Syndrome. 
Treister R, Trudeau JJ, Van Inwegen R, Jones JK, and Katz NP.  2016.  Development and 
feasibility of the misuse, abuse, and diversion drug event reporting system (MADDERS®).  Am J 
Addict.  25: 641–651. 
Varni JW, Sherman SA, Burwinkle TM, Dickinson PE, Dixon P.  2004.  The PedsQL Family 
Impact Module: preliminary reliability and validity.  Health Qual Life Outcomes.  2:55. 

Protocol ZYN2-CL-016.05 
26 April 2020 
 
Confidential 
90 
Viveros MP, Marco EM, et al.  2005.  Endocannabinoid system and stress and anxiety responses.  
Pharmacology, Biochemistry and Behavior.  81:331-342. 
Watanabe K, Itokawa Y, Yamaori S, Funahshi T, Kimura T, Kaji T, Usami N, Yamamoto I.  
2007.  Converstion of cannabidiol to Δ9-tetrahydrocannabinol and related cannabinoids in 
artificial gastric juice, and their pharmacological effects in mice.  Forensic Toxicol.  25:16-21. 
Wei Q, Liu L, Cong Z, Wu X, Wang H, Qin C, Molina P, Chen Z.  2016.  Chronic Δ(9)-
Tetrahydrocannabinol Administration Reduces IgE(+)B Cells but Unlikely Enhances Pathogenic 
SIVmac251 Infection in Male Rhesus Macaques of Chinese Origin.  J Neuroimmune Pharmacol.  
11(3):584-591.  
Winton-Brown TT, Allen P, Bhattacharyya S, Borgwardt SJ, Fusar-Poli P, Crippa JA, Seal ML, 
Martin-Santos R, Ffytche D, Zuardi AW, Atakan Z, McGuire PK.  2011.  Modulation of auditory 
and visual processing by delta-9-tetrahydrocannabinol and cannabidiol: an FMRI study.  
Neuropsychopharmacology.  36(7):1340-1348. 
Zhang L, Alger BE.  2010.  Enhanced endocannabinoid signaling elevates neuronal excitability 
in fragile X syndrome.  J Neurosci.  30(16):5724-5729. 
Zuardi AW, Cosme RA, Graeff FG, Guimarães FS.  1993a.  Effects of ipsapirone and 
cannabidiol on human experimental anxiety.  J Psychopharmacol.  7(Suppl 1):82-88. 
Zuardi AW, Crippa JA, Hallak JE, Pinto JP, Chagas MH, Rodrigues GG, Dursun SM, Tumas V.  
2009.  Cannabidiol for the treatment of psychosis in Parkinson's disease.  J Psychopharmacol.  
23(8):979-983. 
Zuardi A, Crippa J, Dursun S, Morais S, Vilela J, Sanches R, Hallak J.  2010.  Cannabidiol was 
ineffective for manic episode of bipolar affective disorder.  J Psychopharmacol.  24(1):135-137. 
Zuardi AW, Crippa JA, Hallak JE, Moreira FA, Guimarães FS.  2006b.  Cannabidiol, a Cannabis 
sativa constituent, as an antipsychotic drug.  Braz J Med Biol Res.  39(4):421-429. 
Zuardi AW, Shirakawa I, Finkelfarb E, Karniol IG.  1982.  Action of cannabidiol on the anxiety 
and other effects produced by delta 9-THC in normal subjects.  Psychopharmacology (Berl).  
76(3):245-250.