A Global Multicenter, Randomized, Double-blinded, Propofol-controlled, Phase 3 Clinical Study to Evaluate the Efficacy and Safety of HSK3486 Injectable Emulsion for Induction of General Anesthesia in Adults Undergoing Elective Surgery
Phase: PHASE3
Conditions: General Anesthesia
Sponsor: Haisco-USA Pharmaceuticals, Inc.

Cover Page 
Protocol V9.0 HSK3486-309 
 
TITLE OF STUDY: A Global MulƟcenter, Randomized, Double-blinded, Propofol-controlled, 
Phase 3 Clinical Study to Evaluate the Eﬃcacy and Safety of HSK3486 Injectable Emulsion for 
InducƟon of General Anesthesia in Adults Undergoing ElecƟve Surgery 
 
NCT NUMBER: NCT05486416 
DOCUMENT DATE: 28 FEBRUARY 2023 

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Protocol
A Global Multicenter, Randomized, Double-blinded, Propofol-
controlled, Phase 3 Clinical Study to Evaluate the Efficacy and 
Safety of HSK3486 Injectable Emulsion for Induction of General 
Anesthesia in Adults Undergoing Elective Surgery 
Protocol Date: 28 Feb 2024 
Protocol Version: 9.0 
Investigational Product: HSK3486 
Protocol Reference Number: HSK3486-309 
Sponsor: 
Haisco-USA Pharmaceuticals, Inc 
Information described herein is confidential and may be disclosed only with the express 
written permission of the Sponsor. 

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SUMMARY OF CHANGES
The following is a high-level summary of changes made in protocol Version 9.0. The detailed 
changes (existing text and updated text) are provided in Appendix 14. 
Inclusion 9 and Exclusion 3.d). have been updated for clarification

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INVESTIGATOR AGREEMENT 
I have read the following protocol and agree to conduct the study as described herein. 
[Name, Qualification(s)]
Date
Principal Investigator

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SYNOPSIS 
Title of study: A Global Multicenter, Randomized, Double-blinded, Propofol-controlled, Phase 3 Clinical Study 
to Evaluate the Efficacy and Safety of HSK3486 Injectable Emulsion for Induction of General Anesthesia in 
Adults Undergoing Elective Surgery 
Indication: Induction of general anesthesia in adult subjects undergoing elective surgery with endotracheal 
intubation 
Number of study centers: Up to approximately 40 sites in the US and EU will be included in this study. 
Development phase: Phase 3
Objectives:
Primary objective: 
To demonstrate HSK3486 0.4/0.2 mg/kg (0.4 mg/kg intravenous [IV] slow injection for the first dose, an
additional 0.2 mg/kg if needed) is non-inferior to propofol 2.0/1.0 mg/kg (2.0 mg/kg IV slow injection for first
dose, an additional 1.0 mg/kg if needed) in success of induction of general anesthesia in adults undergoing
elective surgery.
Secondary objectives: 
Key secondary objectives: 
To confirm that HSK3486 0.4/0.2 mg/kg leads to statistically significant less injection-site pain in all
compared to propofol 2.0/1.0 mg/kg during the induction of general anesthesia in adults undergoing elective
surgery.
To demonstrate HSK3486 0.4/0.2 mg/kg provides better anesthetic effects compared to propofol 2.0/1.0
mg/kg without significant cardiac and respiratory depression in conjunction with other routinely used
preinduction and maintenance anesthetic agents in the induction of general anesthesia in adults undergoing
elective surgery.
Additional Efficacy Objective: 
To evaluate HSK3486 0.4/0.2 mg/kg induction time in general anesthesia compared to propofol.
To evaluate HSK3486 0.4/0.2 mg/kg time to the disappearance of eyelash reflex compared to propofol.
To confirm that HSK3486 0.4/0.2 mg/kg leads to statistically significant less moderate injection-site pain
compared to propofol 2.0/1.0 mg/kg during the induction of general anesthesia in adults undergoing elective
surgery.
To better characterize that HSK3486 0.4/0.2 mg/kg leads to statistically significant less average NRS scales in
injection-site pain compared to propofol 2.0/1.0 mg/kg during the induction of general anesthesia in adults
undergoing elective surgery.
Safety: 
To evaluate the overall safety profile of HSK3486 compared to propofol.
Pharmacokinetic:
To characterize the HSK3486 population pharmacokinetic (PK) profile.

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Methodology/study design: 
This is a global multicenter, randomized, double-blinded, propofol-controlled, phase 3 clinical study to evaluate 
the efficacy and safety of HSK3486 for induction of general anesthesia in adults undergoing elective surgery with 
endotracheal intubation.  
After screening, eligible subjects will be randomized in a 2:1 ratio to receive either HSK3486 0.4/0.2 mg/kg (i.e., 
0.4 mg/kg IV slow injection over 30 [±5] seconds followed by an additional 0.2 mg/kg dose over 10 [±2] seconds 
if needed) or propofol 2.0/1.0 mg/kg (i.e., 2.0 mg/kg IV slow injection over 30 [±5] seconds followed by an 
additional 1.0 mg/kg dose over 10 [±2] seconds if needed) in a blinded manner. Enrolled subjects will be stratified 
by American Society of Anesthesiologists Physical Status (ASA-PS; I-II and III-
2). Endotracheal intubation will be performed after adequate anesthetic 
Appendix 1) and 
administration of neuromuscular blocking agent.  
On Day 1, premedication is allowed prior to induction except for sedative-hypnotics, analgesics (e.g., opioids, 
NSAIDs, APAP), or any medications that relieve pain (e.g., Gabapentin), unless otherwise specified in the 
protocol. Premedication should be recorded if used. 
Prior to administration of the study drug in the operating room, the preoperative readiness of each subject will be 
4 L/min) at least 2 minutes before 
study drug administration. Subsequently, the investigator can adjust the oxygen flow according to the specific 
situation of the subject and maintenance IV solution (normal saline [NS], lactated ringer’s [LR], or 5% dextrose) 
will be administrated through IV infusion. Throughout the preinduction and induction periods, a timing device 
must be used to allow accuracy and sequencing of necessary assessments.  

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Figure 1
Study design

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Preinduction period:
Obtain vital signs (heart rate [HR], respiratory rate [RR], oxygen saturation [SpO2], systolic blood pressure
[SBP], diastolic blood pressure [DBP], mean arterial pressure [MAP]), Temperature [Temp]; baseline value
will be the measurement immediately prior to initiation of study drug administration. Attach 3-lead or 5-lead
electrocardiogram (ECG) to subject and monitor continuously. In the event an abnormality is noted with 3-
lead or 5-lead ECG, clinical significance of an abnormality should be documented, and the investigators will
decide whether to add a 12-lead ECG.
Attach bispectral index (BIS [Appendix 2]) monitor to subject. Record the three most recent BIS values
preceding midazolam administration.
Subjects will receive IV midazolam at dose 0.04 mg/kg, up to 3 mg maximum over 15 [±2] seconds at 5
minutes [±30 seconds] prior to initiation of induction agents as premedication. The midazolam dose can be
reduced according to patient’s age and comorbidities as per the anesthetist’s discretion. The start and end time
of midazolam administration should be recorded. The end time of midazolam administration will begin the
window to start of IP.
Subjects will receive preinduction IV fentanyl at a dose of 1 mcg/kg rounded up to the nearest 25 mcg, up to
100 mcg maximum over 15 [±2] seconds at 2 minutes [±10 seconds] prior to initiation of induction agents.
The fentanyl dose can be reduced according to patient’s age and comorbidities as per the anesthetist’s
discretion. The start and end time of fentanyl administration should be recorded. The end time of fentanyl
administration will begin the window to start of IP.
Note: Throughout the preinduction and induction periods, a timing device must be used to allow accuracy and
sequencing of necessary assessments.
Information about any adverse events (AEs) and concomitant medications will be recorded.
Induction period of general anesthesia:
The induction of general anesthesia will be performed as follows:
Monitor vital signs (HR, RR, SpO2, SBP, DBP, MAP, Temp) continuously. HR, SBP, DBP, MAP, Temp
should be recorded once every 2 minutes [±30 seconds], SpO2 value should be recorded once every 1 minute
[±15 seconds] from the start of study drug administration (for every time point) for 30 minutes post start of
study drug administration, for the judgment of key secondary endpoint. RR should be recorded once every 2
minutes [±30 seconds] from the start of study drug administration until initiation of administration of
rocuronium.
Note: Vital signs (HR, RR, SBP, DBP, MAP, Temp) monitor should be set to cycle every 1 minute to obtain 
vitals at least every 2 minutes (in case cycle requires longer than 1 minute).  
Administer IV study drug (HSK3486 0.4 mg/kg or propofol 2.0 mg/kg) into vein located on the back of right
or left hand (this IV location is strongly preferred rather than mandatory) at a port closest to the IV cannula
(IV injection time: 30 [±5] seconds).
The MOAA/S will be evaluated every 30 [±10] seconds after end of study drug administration until MOAA/S
1 is reached. Timepoints for MOAA/S 
 within 1 to 40 seconds should be recorded.
If MOAA/S is still >1 at 1 minute [±10 seconds] post end of study drug administration, a top-up dose
of 50% of the initial calculated dose of study drug will be given to the subject (IV injection time: 10

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[±2] seconds). Start and end times of top-up dose administration will be recorded. The MOAA/S will 
be evaluated every 30 [±10] seconds post end of the top-up dose administration. 
If MOAA/S is still >1 at 2 minutes [±10 seconds] post end of the top-up dose administration, then the
rescue drug, propofol, will be given (in both treatment groups). The rescue dose is prepared as the
initial calculated propofol dose (100%) and administered per propofol guidelines. The MOAA/S will
be evaluated every 30 [±
1 is
reached.
Administration of study drug must be initiated 5 minutes [±30 seconds] post midazolam preinduction
medication administration and at 2 minutes [±10 seconds] after preinduction fentanyl administration
stop time. Top-up dose should be administered within 10 seconds once MOAA/S is evaluated >1.
Injection-site pain is evaluated verbally by Numeric Rating Scale (NRS; Appendix 3). Upon initiation of study
drug administration, the investigator should immediately ask the subject to rate his or her pain at injection-site.
As a general guidance, the evaluation should be done multiple times with the first evaluation occurring typically
within 15 seconds after initiation of study drug injection and monitored until successful induction (MOAA/S
-10), shall be recorded.
The maximum (highest value) injection-site pain will be recorded in EDC as NRS.
Post the end of initial study drug administration, the eyelash reflex will be evaluated every 30 [±10] seconds
until loss of eyelash reflex. If there is a top-up dose, eyelash reflex will be evaluated every 30 [±10] seconds
post end of the top-up dose administration until loss of eyelash reflex. If there is rescue drug given, eyelash
reflex will be evaluated every 30 [±10] seconds post end of the rescue drug until loss of eyelash reflex. The
time of loss of eyelash reflex should be recorded. Timepoints for loss of eyelash reflex within 1 to 40 seconds
should be recorded.
BIS will be monitored continuously; record baseline BIS value prior to administering study drug. BIS values
will be collected at the following timepoints from start of initial study drug administration: every 30
[±10] seconds for 5 minutes, then every 2 minutes [±30 seconds] for 30 minutes and then every 30 [±2]
minutes for the duration of the surgery.
Monitor 3-lead or 5-lead electrocardiogram (ECG) continuously. Abnormalities that are clinically significant
or not clinically significant should be recorded. The investigators will decide whether to add a 12-lead ECG.
 mg/kg) is to be administered for
neuromuscular blockade to perform endotracheal intubation. The start and end times of rocuronium bromide
administration should be recorded. RR should be collected until administration of rocuronium.
Note: For subjects with BMI>40 kg/m2, the rocuronium dose may be modified per investigator discretion.
Note: 
 kg/m2: If the exact calculated Rocuronium dose cannot be drawn, round up
or down to the nearest mg (whole number) depending on investigator preference/standard of care.
Note: Additional IV rocuronium is allowed per investigator’s discretion only in response to clinical symptoms
during tracheal intubation, such as gag reflex and movement et al, or during surgical procedures. The start and
end times of additional rocuronium bromide administration should be recorded.
Intubate subject once neuromuscular blockade has taken effect; if using twitch monitor, intubate once no
twitches are noted. The start and end times of first and subsequent intubation attempts should be recorded.

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During endotracheal intubation, evaluate and record clinical symptoms and/or signs for inadequate depth of
anesthesia, such as lacrimation, movement, vomiting, coughing, laryngospasm, bucking, swallowing reflex
and/or bronchospasm, etc. for at least 15 minutes from start of study drug administration.
Between start of study drug administration and prior to the administration of rocuronium bromide, evaluate
and record respiratory depression.
Note: Respiratory depression includes apnea, defined as absence of thoracic movement lasting >30 seconds,
prior to the administration of rocuronium bromide, or hypoxia, defined as SpO2 <90% lasting >30 seconds, or
life-threatening apnea or hypoxia requiring immediate intervention.
Evaluate and record cardiac depression from start of study drug administration until the subject leaves the
operating room.
Note: Cardiac depression is defined as SBP <90 mmHg lasting >2 minutes plus requiring medical intervention
such as inotropes, vasopressors, or IV fluid resuscitation, or life-threatening hypotension requiring immediate
intervention.
Sevoflurane (an inhalation anesthetic agent) will be used for maintenance of general anesthesia. Initiate
sevoflurane within 60 seconds after successful endotracheal intubation. The end-tidal concentration of
sevoflurane should be 1.5-2.0 % (The start and end time of administration should be recorded).
Information about any adverse events (AEs) and concomitant medications will be recorded.
Maintenance period of general anesthesia:
During the maintenance period of general anesthesia, the inhalational anesthetic agent sevoflurane will be
used according to routine clinical practice, and the end-tidal concentration of sevoflurane should be 1.5-2.0 %
within 15 minutes after initiation of study drug administration and can be adjusted to the desired effect after
that. Propofol should not be used at any time all throughout the maintenance period.
During the maintenance period, end-tidal sevoflurane concentration will be monitored per standard of care.
The BIS will be monitored continuously per standard of care, and BIS values will be collected at the following
timepoints from start of initial study drug administration: every 30 [±10] seconds until 5 minutes, then every 2
minutes [±30 seconds] from 5 minutes until 30 minutes and then every 30 [±2] minutes for the duration of the
surgery.
Information about any adverse events (AEs) and concomitant medications will be recorded.
Additional IV fentanyl or other analgesic drug may be administered for intraoperative analgesia only after
initiation of sevoflurane, preferably after 15 minutes following. The start and end time of administration
should be recorded.
Patient management during surgery needs to follow routine best practice which includes Antiemetics
Ondansetron, or other 5 HT-3 antagonists and/or Dexamethasone.
Follow-up period (6 hours Post Study Drug; 24 hours Post Study Drug Administration Phone Contact (Day 
2); Day 8 Phone Contact): 
6 hours post study drug follow-up 
Assess vital signs (HR, RR, SpO2, SBP, DBP, MAP, Temp) at 6 [±2] hours post start study drug
administration.

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Note: If surgery lasts >4 hours, vital signs, clinical laboratory tests and 12-ECG should be obtained 1[+1]
hour after completion of surgery. Duration of surgery is defined as time from study drug administration to 
time of transfer from operating room to recovery room or PACU. 
Clinical laboratory tests (including hematology, blood chemistry, and urinalysis) will be obtained at 6 [±2]
hours post start study drug administration for shipment to central laboratory.
Obtain 12-lead ECG at 6 [±2] hours post start study drug administration.
In the post-anesthesia care unit (PACU), once the subject is alert and oriented, repeat NRS for recall of pain at
time of study drug administration and assess surgical awareness with recall using the Brice Awareness
Questionnaire (Appendix 4). The assessment time and result should be recorded.
Information about any AEs and concomitant mediations will be collected and recorded.
After the surgery, subjects may remain in the hospital or observation unit if required based on standard of care
and the clinical situation; however, if the subject is clinically stable after the 6 hour follow-up assessments and
appropriate to be discharged home per the judgement of the investigator and surgeon, the subject may be
released with supervision by a family member or friend.
For the 24-hour follow-up (Day 2) phone contact 
Subjects will be evaluated by a follow-up telephone call 24 [±6] hours after study drug administration.
Information about any AEs and concomitant mediations will be collected and recorded.
Day 8: Phone Contact 
Subjects will be evaluated by a follow-up telephone call 7 [±2] days after surgery.
Surgical awareness with recall will be re-assessed using the Brice Awareness Questionnaire.
Information about any AEs and concomitant medications will be collected and recorded.
Population PK study:
All eligible subjects in this clinical study will be required to provide venous blood samples for population PK
study per time schedule planned (Appendix 5).
Data monitoring committee: 
An independent data monitoring committee (DMC) will be involved in the conduct of the study to ensure the 
safety of all subjects who have been administered study drug. The DMC will consist of 3 unblinded members: 2 
anesthesiologists with appropriate clinical expertise and 1 statistician. The DMC will review all available 
unblinded safety information at approximately 30% enrollment. Additionally, enrollment will be immediately 
suspended after one death during the study where a clear alternative cause is not readily apparent (i.e., deemed 
definitely or likely related to study drug); after 4 (4/399 [1%] ) non-fatal serious adverse events (SAEs) where a 
clear alternative cause is not readily apparent (i.e., deemed definitely or likely related to study drug); or after 8 
(8/399 [2%]) severe AEs of special interest (AESIs; Common Terminology Criteria for Adverse Events [CTCAE] 
Grade 3 or 4) of severe hypotension, bradycardia, hypoxia due to respiratory depression, or QTc prolongation 
occurring within 15 minutes of study drug administration, where a clear alternative cause is not readily apparent 

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interventions. If these criteria are met, enrollment will be temporarily suspended while the DMC convenes to 
review all available unblinded data. Based on recommendations from the DMC, the Sponsor can terminate the 
study or resume enrollment if measures can be taken to effectively address (i.e., mitigate) the risk to safely 
continue the study, such as revising inclusion/exclusion criteria or study procedures. The final decision to suspend 
enrollment or proceed will be made by the Sponsor after consultation with the DMC members and taking into 
consideration their recommendations. Conduct of the DMC is described in the DMC Charter.    
Number of subjects: A total of 399 subjects; 266 subjects in HSK3486 group and 133 subjects in propofol group. 
Diagnosis and main criteria for inclusion and exclusion: 
Inclusion criteria: 
Subjects must satisfy all of the following criteria at the screening and Day 1 visit: 
1.
Subjects undergoing elective surgery (non emergency, non cardiothoracic, and non intracranial surgery,
anticipated to last at least 1 hour) requiring endotracheal intubation and inhalation general anesthesia during
the maintenance period. Duration of surgery is defined as time from study drug administration to time of
transfer from operating room to recovery room or PACU.
2.
-PS I to IV (Appendix 6). For ASA-PS IV subjects, clinical
status must be optimized at time of preoperative anesthesia evaluation per judgement of the anesthesiologist.
3.
2.
4.
2 
2% in ambient air; 
5.
For all women of childbearing potential, negative serum pregnancy test within the screening period and
negative urine pregnancy test at baseline (Day 1). Additionally, women of childbearing potential* and male
subjects with female partners of childbearing potential must agree to use effective contraception as defined in
7.3.4 from the time of consent until 30 days post study drug administration.
6.
For subjects with known hypothyroidism and/or on thyroid-hormone replacement treatment (i.e., thyroxine),
or subjects suspected to have thyroid dysfunction based on clinical history and physical exam, a TSH must be
drawn and be within normal levels.
7.
Capable of understanding the procedures and methods of this study, willing to sign an Informed Consent
Form, and able to complete this study in strict compliance with the study protocol.
8.
Willing to comply with the site’s COVID guidelines and testing requirements as applicable.
9.
Patients with psychiatric/mental disorders must be considered stable on treatment (e.g., SSRIs, SNRIs, TCAs,
MAOIs, psychotherapy) per investigator judgement, and no hospitalizations and urgent care due to the
underlying psychiatric pathology for at least 12 months.
* Women are considered of childbearing potential until becoming post-menopausal, unless she had a documented
hysterectomy or bilateral oophorectomy / salpingo-oophorectomy. A woman is considered to be post-menopausal
if she had no menses for at least 12 consecutive months (without an alternative medical cause).

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Exclusion criteria:
Subjects will be excluded from the study if they satisfy any of the following criteria at the screening and Day 1
visit: 
1.
Contraindications to deep sedation/general anesthesia or a history of adverse reaction to sedation/general
anesthesia.
2.
Known to be allergic to eggs, soy products, opioids and their antidotes, or propofol; subjects having
contraindications to propofol, opioids, and their antidotes. In cases where the only previous reaction to opioids
was itching or nausea, subjects need not be excluded if the investigator believes the subject is not truly
allergic to opioids.
3.
Medical condition or evidence of increased sedation/general anesthesia risk as follows:
a)
Cardiovascular disorders: uncontrolled hypertension (SBP >160 mmHg and/or DBP >100 mmHg) with or
without antihypertensive therapy (antihypertensive therapy should be stable for 1 month prior to
screening), serious arrhythmia (including the subjects with implanted pace makers), unstable heart failure,
Adams-Stokes syndrome (i.e., syncope or near syncope due to cardiac arrythmia), unstable angina,
myocardial infarction occurring within 6 months prior to screening, history of tachycardia/bradycardia
requiring medications, third degree atrioventricular block or QT interval corrected for HR using
Fridericia’s formula (QTcF) 
 
.
b) History of severe obstructive lung disease (i.e., forced expiratory volume in 1 second [FEV1] <50%
predicted), history of bronchospasm requiring treatment in a hospital emergency room or hospitalization
occurring within 3 months prior to screening, developing acute respiratory tract infection within 2 weeks
prior to baseline (such as symptoms of fever, shortness of breath, wheezing, nasal congestion, and cough).
c)
Cerebrovascular disease: subject with a history of serious craniocerebral injury, convulsion, seizure
disorder, intracranial hypertension, cerebral aneurysm, or stroke.
d) Patients with psychiatric/mental disorders who have not been on a stable treatment regimen (e.g., SSRIs,
SNRIs, TCAs, MAOIs, psychotherapy) per investigator judgement, for at least 12 months or who have
been hospitalized or had emergent/urgent care due to the underlying psychiatric pathology within the last
12 months.
e)
Uncontrolled clinically significant conditions of liver (e.g., severe hepatic insufficiency defined as Childs-
Pugh class C), kidney, gastrointestinal tract, blood system, nervous system, or metabolic system diseases,
judged by the investigator to be unsuitable for involvement in the study.
f)
History of uncontrolled diabetes in the opinion of the investigator.
g) History of alcohol abuse within 3 months prior to screening, where alcohol abuse refers to daily alcohol
drinking >2 units (1 unit = 360 mL of beer or 45 mL of spirit with a strength of 40% or 150 mL of wine).
h) History of drug abuse that, in the opinion of the investigator, may confound the interpretation of safety or
efficacy in a study subject.
i)
For subjects with known hypothyroidism and/or on thyroid-hormone replacement treatment (i.e.,
thyroxine), or subjects suspected to have thyroid dysfunction based on clinical laboratory and physical
exam who has a TSH value outside the normal range.

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4.
Management risks of respiratory tract and judged by the investigator to be unsuitable for inclusion in the study
as follows:
a)
Asthma must be stable: stable doses of asthma medications for the past 6 months, no requirement for
rescue inhalers or oral steroids within past 6 months, not evaluated in emergency department, urgent care,
or hospitalized for an asthma attack within past 1 year.
b) History (or family history) of malignant hyperthermia.
c)
Any previous failure of tracheal intubation.
d) Judged to have a difficult airway for endotracheal intubation in the opinion of the Investigator based on
parameters such as modified Mallampati score (Grade III or IV [Appendix 7]), neck mobility, short
thyromental distance, and/or history of difficult intubation.
5.
Any medication that has the potential to interact synergistically with propofol or HSK3486, including but not
limited to all sedatives and hypnotics (e.g., benzodiazepines and opioids) taken within 5 half-lives prior to
Day 1.
6.
Laboratory parameters measured at screening with the following levels:
a)
9/L
b) Platelet count <80 x 109/L
c)
Hemoglobin <90 g/L (without blood transfusion within 14 days)
d)
e)
f)
 mL/min
7.
Female subjects with a positive pregnancy test at screening (serum) or baseline (urine); lactating subjects; any
subject planning to get pregnant within 1 month after the study (including the male subject’s partner).
8.
Judged by the investigator to have any other factors that make the subject unsuitable for participation in the
study.
Test products, dose, and mode of administration: 
Name: HSK3486 injectable emulsion  
Dose, route, frequency: 0.4 mg/kg, IV slow injection over 30 [±5] seconds, preoperatively; 1 top up dose (0.2 
mg/kg), IV injection over 10 [±2] seconds permitted if needed.  
For elderly subjects 
, the dose will be automatically adjusted to a 25% dose reduction. 
According to the judgment of the investigator, the dose can be further reduced up to 50% of the calculated dose. 
The administration time should be extended up to 1 minute for this population (IV slow injection over 30-60 
seconds).  
For ASA grade 3-4 subjects, the dose can be reduced by 25-50% per investigator discretion. The administration 
time should be extended up to 1 minute for these populations (IV slow injection over 30-60 seconds). 

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2, total body weight (TBW) will be used to determine HSK3486 dose; for subjects 
with BMI >40 kg/m2, lean body weight (LBW) will be used to determine HSK3486 dose, and rescue dose. LBW 
should be calculated using the Janmahasatian formula included below. 
The Janmahasatian Formula: 
•
For males: LBW = (9270 x TBW (kg)) / (6680 + (216 x BMI))
•
For females: LBW = (9270 x TBW (kg)) / (8780 + (244 x BMI))
away from light and should not be frozen. It should 
be shaken well before use. 
Reference therapy, dose, dose form, and mode of administration: 
Name: Propofol injection 
Dose, route, frequency: 2.0 mg/kg, IV slow injection over 30 [±5] seconds, preoperatively; 1 top up dose (1 
mg/kg), IV injection over 10 [±2] seconds is permitted if needed.  
, the dose will be automatically adjusted to a 25% dose reduction. 
According to the judgment of the investigator, the dose can be further reduced by up to 50% of the calculated 
dose. The administration time should be extended up to 1 minute for this population (IV slow injection over 30-60 
seconds). 
For ASA grade 3-4 subjects, the dose can be reduced by 25-50% per investigator discretion. The 
administration time should be extended up to 1 minute for these populations (IV slow injection over 30-60 
seconds).  
2, TBW will be used to determine propofol dose; for subjects with BMI >40 
kg/m2, LBW will be used to determine propofol dose, and Rescue dose. LBW should be calculated using the 
Janmahasatian formula included below. 
The Janmahasatian Formula: 
•
For males: LBW = (9270 x TBW (kg))/(6680 + (216 x BMI))
•
For females: LBW = (9270 x TBW (kg))/(8780 + (244 x BMI))
 Propofol will be stored between 4 C and 
40  to 
 It should 
be shaken well before use.  

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The rescue dose, propofol, is prepared as 100% of the initial calculated dose and administered per propofol 
guidelines above. The rescue dose administration time should be extended up to 1 minute (IV slow injection over 
30-60 seconds) 
-4 subjects who require a dose reduction.
Duration of subject participation in study: 
The sequence and maximum duration of the study periods per subject will be as follows: 
Screening: up to 14 days prior to the surgery and including the day of surgery (if needed)
Treatment: on the day of surgery
Follow-up phone contacts: 24 hours post study drug administration and 7(±2) days post-surgery
Total duration for each subject is expected to be 22 [±2] days (including screening and 1 week follow up
telephone call).
Efficacy endpoints:  
Primary efficacy endpoint: 
Success rate of general anesthesia induction: A successful general anesthesia induction will meet both of the
following conditions:
a)
b) One or less top-up doses required without using any rescue drugs.
Secondary efficacy endpoints: 
Key secondary endpoints: 
The proportion of subjects with any injection-site pain at time of drug administration on the Numeric Rating
The proportion of subjects with successful induction who maintain the desired depth of anesthesia for general
elective surgery, and without significant cardiac and respiratory depression within15 minutes post initiation
start of study drug administration, or up to the start of second tracheal intubation attempt if it is a difficult
condition and not beyond 15 minutes post initiation start study drug administration, defined by all the
following conditions:
a)
Desired depth of anesthesia for general elective surgery is defined if all following criteria are met:
i)
No clinical signs of inadequate depth of anesthesia, such as lacrimation, movement, vomiting,
coughing, laryngospasm, bucking, swallowing reflex or bronchospasm etc.
ii) No blood pressure (SBP, DBP, or MAP) increases more than 20% from baseline in response to any
major operational procedures or noxious stimulus in defined period.
iii) Subjects maintain desired depth of anesthesia for general elective surgery with BIS as an objective
assessment (after reaching initial lowest value, BIS remains sustainable level at not more than 60).
Note: Target BIS 40 to 60 for general anesthesia (Appendix 2).
The BIS sustainable level at not more than 60 is defined as not more than 1 episode with BIS value
observed >60 after reaching initial lowest value, in defined period.

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Note: It is strongly recommended that each site have available video laryngoscopy, or an acceptable 
alternative, for unanticipated difficult tracheal intubation. If more than one tracheal intubation attempt is 
made, the start and end times of first and subsequent intubation attempts should be recorded. 
b) No significant respiratory depression, such as apnea, prior to the administration of rocuronium bromide.
Note: For the composite endpoint, respiratory depression includes apnea, defined as absence of thoracic
movement lasting >30 seconds, or hypoxia, defined as SpO2 <90% lasting >30 seconds, or life-threatening
apnea or hypoxia requiring immediate intervention.
c)
No significant cardiac depression indicated by blood pressure decrease that requires intervention, i.e.,
vasopressors and/or IV fluid resuscitation.
Note: For the composite endpoint, cardiac depression is defined as SBP <90 mmHg lasting >2 minutes
plus requiring medical intervention such as inotropes, vasopressors, or IV fluid resuscitation, or life-
threatening hypotension requiring immediate intervention.
Other secondary endpoints: 
Time to successful induction of general anesthesia: Time from the end of the first administration of the study
Time to the disappearance of eyelash reflex: Time from the end of the first administration of the study drug to
the disappearance of eyelash reflex.
The proportion of the subjects with any moderate injection-site pain at time of drug administration on the
Numeric Rating Scale (NRS >4).
The average injection site pain at time of drug administration of HSK3486 compared to propofol on NRS.
Safety endpoints: 
Safety assessments include Adverse events (AE), adverse events of special interest (AESI), and serious
adverse events (SAE) clinical laboratory test results, vital signs (supine HR, SBP and DBP, MAP, RR, and
SpO2), ECG findings, physical examination findings, and administration of additional medications, or any
interventions including medical interventions, e.g., administration of vasoactive drugs to treat clinically
relevant changes in blood pressure.

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Pharmacokinetic endpoints:
Plasma HSK3486 concentration at scheduled timepoints (Appendix 5). 
Sample size evaluation:
A total of 399 subjects (266 in HSK3486 0.4/0.2 mg/kg group and 133 in propofol 2.0/1.0 mg/kg group) need to 
be enrolled 2:1 into this study based on the following assumptions: 
For the primary endpoint, a sample size of 215 patients will give at least 90% power (providing the primary
endpoint is statistically significant) assuming that type I error is 0.025 (1-sided), the success rate of general
anesthesia induction of HSK3486 and propofol are both 97%, and non-inferiority margin (NIM) is -8%.
For the key secondary endpoint of incidence of injection-site pain, a sample size of 365 patients will give 90%
power (providing the primary endpoint is statistically significant) 
0.015 (2-sided) and the
proportion of subjects who meet the endpoint criteria of any injection-site pain are 6.8% and 20.5% for
HSK3486 and propofol, respectively.
For the key secondary composite endpoint, a sample size of 338 patients will give 90% power (providing the
0.035 (2-sided), the
proportion of subjects with successful induction, maintained desired depth of anesthesia for general elective
surgery, without significant cardiac depression within the 15-minute post initiation start of study drug
administration observation period and no significant respiratory depression (prior to administration of
rocuronium bromide) of HSK3486 and propofol are 82% and 65%, respectively (i.e., 17% treatment effect).
To power statistical testing for all three endpoints at 90% and provide sufficient safety data, it is decided that 399 
patients will be randomized and treated in this study (266 in HSK3486 0.4/0.2 mg/kg group and 133 in propofol 
2.0/1.0 mg/kg group).  
Statistical methods: 
Statistical analysis sets: 
Full Analysis Set (FAS): All randomized subjects who have received any dose of the study drug (HSK3486 or 
propofol). 
Per Protocol Set (PPS): All subjects from FAS who have completed primary efficacy endpoint measurement will 
be considered for PPS. Subjects with any important protocol deviations will be reviewed after database lock and 
prior to study unblinding and may be excluded from PPS if these important deviations will impact the efficacy 
evaluations. 
Safety Set (SS): Includes all randomized subjects who have received any dose of the study drug and have post-
dose safety assessment data. 

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General principles:
Continuous variables will be summarized by the standard descriptive statistics: number of subjects (n), mean, 
standard deviation (SD), median, minimum, and maximum. Frequency of subjects or events and percentages will 
be summarized in categorical variables. 
Efficacy analysis: 
(a) Primary efficacy analysis
Success rate of general anesthesia induction in both groups and rate difference between groups and its
95% confidence interval (CI) are estimated by Farrington-Manning method in the FAS. The lower limit of
95% CI of rate difference will be compared with the non-inferiority margin of -8% to confirm the
establishment of non-inferiority. FAS subjects who are non-evaluable for anesthesia induction will be
counted as treatment failures.
(b) Secondary efficacy analysis
The 2 key secondary endpoints will be analyzed in parallel. The proportion of subjects with successful
induction, who maintain desired depth of anesthesia for general elective surgery, and without significant
respiratory depression (prior to administration of rocuronium bromide) and cardiac depression between
the time of successful induction and 15 minutes post study drug administration in both groups and the
difference between groups and 95% CI will be calculated. The p-value for comparison between groups
will be obtained based on Chi-square test.
The proportion of the subjects with any injection-
-site pain
, using the same
method as stated above for the secondary composite endpoint.
The median and its 95% CI of time to successful induction of general anesthesia will be provided by
groups using the Kaplan-Meier (KM) method.
Time to the disappearance of eyelash reflex will be analyzed using the similar statistical methods as time
to successful induction of general anesthesia.
The change of BIS during the period of anesthesia post study drug administration up to 15 minutes will be
summarized descriptively by treatment group.
Recall of awareness during surgery, assessed postoperatively, will be evaluated using the Brice Awareness
Questionnaire.
The use of study drugs and rescue drugs will be summarized descriptively by treatment group.
The proportion of subjects with successful induction without non-optimal anesthetic effects within 15
minutes post initiation of administration of study drugs will be analyzed using the similar statistical
methods as for the secondary composite efficacy endpoint.
Safety analysis:
All AEs will be coded according to the MedDRA version 25.0 or later and graded for severity according to 
CTCAE version 5.0. The number and percentage of subjects with treatment-emergent adverse events (TEAEs), 
SAEs, AEs of Special Interest (AESI), TEAEs related to study drug, SAEs related to study drug, TEAEs of Grade 
2 or higher, TEAEs leading to treatment discontinuation, TEAEs leading to study discontinuation, and TEAEs 

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leading to death will be summarized by system organ class (SOC), preferred term (PT), and groups. In addition, 
the severity of TEAEs and relationship to study drug will be summarized by SOC, PT, and groups.  
Laboratory test variables will be summarized by treatment group and visit using descriptive statistics. Shift tables 
between baseline and post-baseline time points will be presented by laboratory test and treatment group. 
Laboratory tests with categorical results that cannot be analyzed by change from baseline or shift table analysis 
will not be included in these summaries but will be listed. 
Descriptive statistics of vital signs, pulse oximetry measurements, and ECG results at each visit will be presented. 
Note: Since injection-site pain has been recorded and analyzed in the efficacy endpoints, it is no longer recorded 
in the Safety endpoints. 
Population PK analysis: 
The plasma concentration data from this study will be pooled with data from other clinical trials of HSK3486 to 
establish a population PK model. This model will be used to evaluate the effects of internal and external covariates 
on the PK of HSK3486. 

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SCHEDULE OF ASSESSMENTS
Screening 
Study 
treatment 
Follow-up visit 
Phone contacts 
Day -13 to 
Day 1p
(prior to 
surgery) 
Day 1 
(day of 
surgery) 
PACU and 
6-hour
Day 2 
24 (±6) hours 
after study drug 
administration 
Day 8 
7 (±2) days post day of 
surgery 
PACU
6 (±2) hours 
24 (±6) 
hours 
7 (±2) 
days 
Written informed 
consent 
X 
Inclusion/exclusion 
criteria 
X 
Verify 
Demographics 
X 
Medical history 
X 
Modified Mallampati 
score assessment 
X 
Physical exam a
X 
Vital signs 
X 
X j 
X k
Height, weight, and 
BMI 
X 
12-lead ECG
X 
X f
X f
Clinical laboratory 
tests 
X lp 
X k 
Serum pregnancy test 
(females)  
X lp 
Urine pregnancy test 
(females)  
X l

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Screening 
Study 
treatment 
Follow-up visit 
Phone contacts 
Day -13 to 
Day 1p
(prior to 
surgery) 
Day 1 
(day of 
surgery) 
PACU and 
6-hour
Day 2 
24 (±6) hours 
after study drug 
administration 
Day 8 
7 (±2) days post day of 
surgery 
PACU
6 (±2) hours 
24 (±6) 
hours 
7 (±2) 
days 
Instruct subject to fast 
minimum of 6 hours 
before surgeryo
X 
Confirm preoperative 
readiness b
X 
Randomization 
Xq 
Start maintenance IV 
infusion (NS, LR, or 
5% dextrose) 
X 
Administration of 
preinduction IV 
midazolam 
X 
Administration of 
preinduction IV 
fentanyl 
X 
Administer oxygen via 
face mask ( 4 L/min)  
X 
Administration of 
study drug c 
X 
Pain NRS d 
X 
X 
Monitor BIS e
X 
Monitor eyelash reflex 
X 
Monitor MOAA/S 
X

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Screening 
Study 
treatment 
Follow-up visit 
Phone contacts 
Day -13 to 
Day 1p
(prior to 
surgery) 
Day 1 
(day of 
surgery) 
PACU and 
6-hour
Day 2 
24 (±6) hours 
after study drug 
administration 
Day 8 
7 (±2) days post day of 
surgery 
PACU
6 (±2) hours 
24 (±6) 
hours 
7 (±2) 
days 
Monitor 3-lead or 5-
lead ECG 
continuously f 
X 
Administration of IV 
rocuronium g
X 
Endotracheal 
intubation g
X 
General anesthesia 
maintenance per 
routine practice 
(sevoflurane must be 
used as inhalational 
agent; record use of 
any alternative drugs) h
X 
Blood sample 
collection for 
population PK study 
X m 
Brice Awareness 
Questionnaire 
X n
X n 
Adverse events and 
prior/concomitant 
medications 
X 
X
X 
X 
X 
X 
Abbreviations: ASA-PS = American Society of Anesthesiologists Physical Status; BIS = bispectral index; BMI = 
body mass index; DBP = diastolic blood pressure; ECG = electrocardiogram; HR = heart rate; IV = intravenous; 
LR = lactated ringer’s; MAP = mean arterial pressure; MOAA/S = Modified Observer’s Assessment of 
Awareness/Sedation; NRS = Numeric Rating Scale; NS = normal saline; OR = operating room; PACU = post-

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anesthesia care unit; PK = pharmacokinetic; RR = respiratory rate; SBP = systolic blood pressure; SpO2 = 
peripheral capillary oxygen saturation. 
a.
Physical examination will include ASA-PS score (Appendix 6).
b.
Subject is hemodynamically stable and has followed preoperative instructions, and there is no evidence of acute
illness such as fever.
c.
Administer IV study drug (HSK3486 0.4 mg/kg or propofol 2.0 mg/kg) on the back of right or left hand vein
(this IV location is strongly preferred rather than mandatory) at a port closest to the IV cannula (IV injection
time: 30 [±5] seconds). The MOAA/S will be evaluated every 30 [±10] seconds after end of injection until
] post end of study drug administration,
a top-up dose of 50% of the initial calculated dose of study drug (either HSK3486 0.2 mg/kg or propofol 1
mg/kg depending on treatment group) will be given to the subject (IV injection time: 10 [±2] seconds) and start
and end time of administration will be recorded. The MOAA/S will be evaluated every 30 [±10] seconds post
end of the top-up dose, If MOAA/S is still >1 at 2 minutes [±10 seconds] post end of the top-up dose, then the
rescue drug, propofol (100% of the initial calculated dose), will be given (in both treatment groups). The
MOAA/S will be evaluated every 30 [±
reached. Administration of study drug must be initiated 5 minutes [±30 seconds] post midazolam preinduction
medication administration stop time. Top-up doses should be administrated within 10 seconds once MOAA/S is
evaluated >1.
d.
Prior to entering the OR, orient the subject to the use of the NRS assessment. Upon initiation of study drug
administration, the investigator should immediately ask the subject to rate his or her pain. As a general
guidance, the evaluation should be done multiple times with the first evaluation occurring typically within 15
seconds after initiation of study drug injection and 
information such as the occurrence and severity of injection pain (NRS 0-10) shall be recorded. The maximum
(highest value) injection-site pain should be recorded. Pain during the injection of study drug in the hand/arm
should be distinguished from unrelated puncture pain from the IV site or due to adhesive tape at the IV site.
When injection pain occurs, the subject’s pain should be graded verbally using the NRS (0-10) and should be
recorded. The subject should be asked to rate the pain on a scale from 0, which is no pain, to 10, which is the
worst pain imaginable. In the PACU, once the subject is alert and oriented, repeat NRS for recall of pain at time
of study drug administration.
e.
At preinduction, attach BIS monitor to subject. Record the three most recent BIS values before midazolam
administration. Also, record baseline BIS value prior to administering study drug. The BIS values will be
collected at the following timepoints at start of initial study drug administration: every 30 [±10] seconds until 5
minutes, then every 2 minutes [± 30 seconds] until 30 minutes and then every 30 minutes [±2] minutes for the
duration of the surgery.
f.
12-lead ECG results collected within 30 days prior to Day 1 may be used to confirm eligibility. Obtain 12-lead
ECG within the screening period only if the subject reports any cardiopulmonary symptoms that warrant an
ECG, in the opinion of the investigator. During the surgery, 3-lead or 5-lead ECG will be monitored
continuously and the investigators will decide whether to add a 12-lead ECG. A 12-lead ECG will be obtained
at 6 [±2] hours post study drug administration and as clinically indicated.
g.
blockade to perform endotracheal intubation. RR collected until initiation of administration of rocuronium.
Intubate subject once neuromuscular blockade (rocuronium) has taken effect; if using twitch monitor, intubate

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once no twitches are noted. The start and end of first and subsequent intubation attempt time should be 
recorded. 
h.
An inhalational anesthetic agent, Sevoflurane (an inhalation anesthetic agent) will be used for maintenance of
general anesthesia. Initiate sevoflurane within 60 seconds after successful endotracheal intubation. The end-tidal
concentration of sevoflurane of should be 1.5-2.0% (The start and end time of administration should be
recorded).
i.
After the surgery, subjects may remain in the hospital or observation unit if required based on standard of care
and the clinical situation; however, if the subject is clinically stable after the 6 hour follow-up assessments and
appropriate to be discharged home per the judgement of the investigator and surgeon, the subject may be
released with supervision by a family member or friend.
j.
On the day of surgery prior to entering the operating room (after the subject has been 
minutes), vital signs (HR, RR, SpO2, SBP, DBP, MAP, Temp) will be collected. During preinduction, obtain
baseline vital signs (baseline value will be the measurement immediately prior to initiation of study drug
administration). During the induction of general anesthesia monitor vital signs (HR, RR, SpO2, SBP, DBP,
MAP, Temp) continuously. HR, SBP, DBP, MAP, Temp should be recorded once every 2 minutes [±30
seconds], SpO2 value should be recorded once every 1 minute [±15 seconds] from the start of study drug
administration (for every time point) for 30 minutes post start of study drug administration, for the judgment of
key secondary endpoint. RR should be recorded once every 2 minutes [± 30 seconds] from the start of study
drug administration until administration of rocuronium. After the surgery, vital signs (HR, RR, SpO2, SBP,
DBP, MAP, Temp) will be assessed at 6 [±2] hours post study drug administration.
k.
At 6 [±2] hours post study drug administration, obtain vital signs (HR, RR, SpO2, SBP, DBP, MAP, Temp) and
clinical laboratory tests (including hematology, blood chemistry, and urinalysis).
l.
Clinical laboratory tests (Appendix 10) include hematology, blood chemistry, and urinalysis; pregnancy tests
for all women of childbearing potential (Negative serum pregnancy test within the screening period and
negative urine pregnancy test at baseline (Day 1)).
m. The blood collection time points for population PK analysis are listed in Appendix 5. Approximately 2 mL of
venous blood is collected at each time point.
n.
The Brice Awareness Questionnaire is administered in the PACU once the subject is alert and oriented. The
questionnaire is repeated at Day 8.
o.
Per investigator discretion, in otherwise healthy patients, clear liquids may be ingested for up to 2 hours prior to
surgery. These liquids should not include alcohol. Examples of clear liquids include, but are not limited to,
water and fruit juices without pulp, carbonated beverages, carbohydrate-rich nutritional drinks, clear tea and
black coffee.
p.
Local labs completed within 30 days prior to Day 1 may be used to confirm eligibility. In these cases, required
screening laboratory samples must be drawn within the screening period and sent to the central laboratory.
q.
Randomization in IXRS can occur the day prior to surgery if needed to accommodate pharmacy, research, or
surgery center hours. Study drug must be administered within 12 hours of preparation.

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Table of Contents 
SUMMARY OF CHANGES ...............................................................................................2
SPONSOR APPROVAL .....................................................................................................3
INVESTIGATOR AGREEMENT.......................................................................................4
SYNOPSIS ..........................................................................................................................5
SCHEDULE OF ASSESSMENTS ....................................................................................21
LIST OF FIGURES ...........................................................................................................29
LIST OF ABBREVIATIONS ............................................................................................30
1.
INTRODUCTION ...............................................................................................32
1.1.
Study Rationale .................................................................................................32
1.2.
Background .......................................................................................................33
1.3.
Benefit-risk Assessment ....................................................................................34
2.
OBJECTIVES AND ENDPOINTS .....................................................................36
2.1.
Objectives ..........................................................................................................36
2.2.
Endpoints ..........................................................................................................37
3.
INVESTIGATION PLAN ...................................................................................39
3.1.
Overall Study Design and Plan Description......................................................39
3.2.
Discussion of Study Design, Including the Choice of Control Groups ............39
3.3.
End of Study Definition ....................................................................................40
3.4.
Selection of Doses in the Study ........................................................................40
3.4.1. 
Selection of Dose for HSK3486 ........................................................................ 40 
3.4.2. 
Selection of Dose for Propofol .......................................................................... 41 
3.5.
Data Monitoring Committee .............................................................................42
4.
SELECTION OF STUDY POPULATION .........................................................43
4.1.
Inclusion Criteria ...............................................................................................43
4.2.
Exclusion Criteria..............................................................................................44
4.3.
Subject Discontinuation from the Study ...........................................................46
4.3.1. 
Screen Failures .................................................................................................. 46 
4.3.2. 
Subject Discontinuation from Study Treatment ................................................ 46 
4.3.3. 
Lost to Follow-up .............................................................................................. 47 
4.3.4.
Replacement Procedures ................................................................................... 48
4.3.5. 
Follow-up of Subjects Prematurely Discontinued from the Study Treatment 
Regimen or Withdrawn from Study ................................................................ 48 
4.4.
Study Stopping Criteria .....................................................................................48
5.
STUDY TREATMENTS ....................................................................................50
5.1.
Treatments Administered ..................................................................................50
5.2.
Preparation, Storage, Handling, and Accountability .........................................50
5.3.
Method of Treatment Assignment ....................................................................52
5.3.1. 
Dose Modification ............................................................................................. 53 
5.4.
Blinding .............................................................................................................53
5.5.
Treatment Compliance ......................................................................................54
5.6.
Preinduction Drugs ...........................................................................................54
6.
CONCOMITANT THERAPIES AND OTHER RESTRICTIONS ....................55
6.1.
Concomitant Therapies .....................................................................................55
6.2.
Prohibited Therapies .........................................................................................56

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7.
STUDY ASSESSMENTS AND PROCEDURES ..............................................57
7.1.
Study Procedures ...............................................................................................57
7.1.1. 
Screening Period (Day -13 to Day 1) ................................................................ 57 
7.1.2.
Day of Surgery (Day 1) ..................................................................................... 58
7.1.3.
Follow-up Period 6 (±2) hour Post Study Drug and 24-hour Post Study Drug 
Phone Contact [Day 2] .................................................................................... 62 
7.1.4. 
Phone Contact (Day 8) ...................................................................................... 63 
7.2.
Efficacy Assessments ........................................................................................64
7.2.1.
Success Rate of Anesthesia Induction............................................................... 64
7.2.2.
Composite Endpoint to Evaluate Desired Depth of Anesthesia........................ 64
7.2.3. 
Injection-site Pain.............................................................................................. 65 
7.2.4. 
Time to Successful Anesthesia Induction ......................................................... 65 
7.2.5. 
Time to Loss of Eyelash Reflex ........................................................................ 66 
7.2.6.
Successful induction without non-optimal anesthetic effects ........................... 66
7.2.7.
Changes in the Bispectral Index ........................................................................ 66
7.2.8. 
Brice Awareness Questionnaire ........................................................................ 66 
7.2.9. 
Use of Study Drugs and Alternative Drugs....................................................... 66 
7.3.
Safety and Tolerability Assessments ................................................................66
7.3.1. 
Adverse Events.................................................................................................. 66 
7.3.2. 
Reporting Serious Adverse Events.................................................................... 67 
7.3.3. 
Adverse Events of Special Interest ................................................................... 68 
7.3.4. 
Contraception and Pregnancy ........................................................................... 70 
7.3.5. 
Clinical Laboratory Evaluations ....................................................................... 70 
7.3.6. 
Vital Signs, Physical Examination, and Other Safety Evaluations ................... 71 
7.3.7. 
Use of Additional Medication or Any Interventions......................................... 72 
7.4.
Pharmacokinetic Analysis .................................................................................73
8.
SAMPLE SIZE AND DATA ANALYSES ........................................................74
8.1.
Determination of Sample Size ..........................................................................74
8.2.
Analysis Populations .........................................................................................74
8.3.
General Considerations .....................................................................................75
8.4.
Efficacy Analysis ..............................................................................................75
8.4.1. 
Primary Efficacy Outcome Measure ................................................................. 75 
8.4.2.
Justification of the Non-inferiority Margin ....................................................... 75
8.4.3. 
Secondary Efficacy Outcome Measures ........................................................... 76 
8.4.4. 
Methods for the Control of Type I Error ........................................................... 76 
8.5.
Safety Analysis .................................................................................................77
8.6.
Pharmacokinetic Analysis .................................................................................78
8.7.
Interim Analysis ................................................................................................78
9.
REFERENCES ....................................................................................................79
10.
APPENDICES .....................................................................................................81
Appendix 1
Modified Observer’s Assessment of Awareness/Sedation (MOAA/S) 
Scale ..................................................................................................................81
Appendix 2 Bispectral Index (BIS).................................................................................82
Appendix 3 Numerical Rating Scale (NRS) for Grading of Injection-site Pain .............83
Appendix 4 Brice Awareness Questionnaire .....................................................................84
Appendix 5
Pharmacokinetic (PK) Blood Samples Collection Timetable ....................86

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Appendix 6
American Society of Anesthesiologists (ASA) Physical Status Grade .....87
Appendix 7
Mallampati Classification ..........................................................................88
Appendix 8
Drugs that May Prolong the QT Interval ...................................................89
Appendix 9
Adverse Event Definitions .........................................................................91
Appendix 10 Clinical Laboratory Evaluations ..................................................................95
Appendix 11 Regulatory, Ethical, and Study Oversight Considerations ...........................97
Appendix 12 Study design (a larger version) ..................................................................101
Appendix 13  IMP/AxMP designation ..........................................................................103
Appendix 14 Detailed Summary of Changes...................................................................104

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LIST OF FIGURES 
Figure 1 
Study design ................................................................................................................ 7
Figure 2  The BIS Index is scaled to correlate with important clinical endpoints during 
administration of anesthetic agent. ............................................................................................... 82
Figure 3  BIS Index Range: A Continuum of Clinical State and EEG Changes ....................... 83 

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LIST OF ABBREVIATIONS
Abbreviation 
Definition
ADR 
adverse drug reaction
AE 
adverse event
AESI
Adverse Event of Special Interest
ASA-PS
American Society of Anesthesiologists Physical Status 
BIS
bispectral index
BMI
body mass index
BP
blood pressure 
CFR
Code of Federal Regulations
CI
confidence interval
CRA
clinical research associate
CRO 
Contract Research Organization
CSA 
clinical study agreement
CTCAE
Common Terminology Criteria for Adverse Events
DBP 
diastolic blood pressure
DMC
data monitoring committee 
DSS
Drug Safety Services 
EC
Ethics Committee
ECG 
electrocardiogram 
eCRF 
electronic Case Report Form 
EDC 
electronic data capture 
EEG 
electroencephalogram
FAS
Full Analysis Set
GCP 
Good Clinical Practice 
HR 
heart rate 
ICF
Informed Consent Form 
ICH 
International Council for Harmonisation 
IRB
Institutional Review Board
IV 
intravenous
IXRS
Interactive Voice/Web Response Technology 
IMP 
Investigational Medicinal Products
kg
kilogram 
L
Liter 
LBW
lean body weight 
LR
Lactated ringer’s 
ISD
Initiation of study drug 
MAP 
mean arterial pressure
mcg 
microgram
MedDRA 
Medical Dictionary for Regulatory Activities 
mg
milligram 

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Abbreviation 
Definition 
min
minute
mL 
milliliter
MOAA/S
Modified Observer’s Assessment of Awareness/Sedation 
NIM
non-inferiority Margin
NRS 
Numeric Rating Scale 
NS
normal saline
NMPA
National Medical Products Administration
NDA
New Drug Application
PACU
post-anesthesia care unit
PD
pharmacodynamic
PK
pharmacokinetic
PPS 
Per Protocol Set
QTcF 
QT interval corrected for heart rate using Fridericia’s formula 
RBC 
red blood cell 
RR 
respiratory rate
SAE
serious adverse event
SAP
Statistical Analysis Plan
SBP 
systolic blood pressure
SpO2 
oxygen saturation 
SS 
Safety Set
SUSAR 
suspected unexpected serious adverse reaction
TBW
total body weight 
TEAE
treatment-emergent adverse event 
Temp 
temperature
ULN 
upper limit of normal 
WBC
white blood cell

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1. INTRODUCTION
Propofol is one of the most widely used intravenous (IV) anesthetics in clinical procedures. It 
induces anesthesia effectively, produces a short duration of anesthesia that allows subjects to 
recover quickly, and is widely used in the induction and maintenance of general anesthesia for 
surgery and out subject surgical procedures. However, propofol also comes with pronounced 
shortcomings, in particular, pain on injection [1], propofol-induced decrease in diastolic blood 
pressure (DBP) and mean arterial pressure (MAP), respiratory depression [2], and excessive lipid 
intake causing subjects to suffer from disorders of lipid metabolism, etc. 
HSK3486 injectable emulsion (hereinafter referred to as HSK3486) is a new IV anesthetic drug 
developed by Sichuan Haisco Pharmaceutical Co., Ltd. HSK3486 is intended to be used in the 
induction of adult sedation/general anesthesia. HSK3486 has a novel structural design that 
provides higher potency compared to propofol, and potentially results in less site pain and 
cardiopulmonary instability. Clinical data to date demonstrated HSK3486 significantly reduces 
pain on injection and reduces hypotensive effects while still providing anesthesia induction rates 
equivalent to propofol. 
1.1. Study Rationale 
This is a global multicenter, randomized, double-blinded, propofol-controlled, Phase 3 clinical 
study to evaluate the efficacy and safety of HSK3486 for induction of general anesthesia in 
adults undergoing elective surgery with endotracheal intubation. The objective of this study is to 
demonstrate that HSK3486 is non-inferior to propofol in the induction of general anesthesia and, 
furthermore, to demonstrate better anesthetic effects (i.e., desired depth of anesthesia) compared 
to propofol with potential for less cardiac and respiratory depression. Clinical signs of inadequate 
depth of sedation during endotracheal intubation include tachycardia, hypertensive response, 
preserved reflexes (e.g., coughing), bronchospasm, movement on painful stimuli, and a 
bispectral index (BIS) score >60 (Appendix 2). Cardiovascular responses have been found to be 
sensitive indicators of noxious stimuli (e.g., endotracheal intubation) during general anesthesia 
[3,4]. The primary endpoint is successful induction of anesthesia defined by Modified Observer’s 
-up doses. A key secondary 
endpoint requires inducing a desired depth of anesthesia without cardiac and respiratory 
depression for 15 minutes post initiation of the study drug administration, which is defined by 
meeting all of the following: no lacrimation, movement, vomiting, coughing, laryngospasm, 
bucking, swallowing reflex and/or bronchospasm, etc.; no blood pressure (BP) increase >20% 
from baseline; and a BIS score <60. Furthermore, subjects must not exhibit a decrease in BP 
requiring intervention, or significant respiratory depression or apnea requiring intervention prior 
to pharmacologic paralysis for intubation. Incorporating these criteria into a composite endpoint 
provides a multidimensional approach to assessing an ideal depth of anesthesia which is 
clinically meaningful and relevant.   

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Results from the Phase 3 HSK3486-302 study showed that fewer subjects treated with HSK3486 
compared to propofol demonstrated inadequate depth of anesthesia for 15 minutes post-dose as 
indicated by an increase in BP >20% or heart rate (HR) >30% from baseline, or BIS score >60 
(HSK3486 24% compared to propofol 46%, p=0.0026). These data support using the composite 
secondary endpoint and thus has the ability to show a statistically significant difference between 
HSK3486 versus propofol in achieving the desired depth of anesthesia for induction of general 
anesthesia.
1.2. Background 
Induction of general anesthesia refers to the transition in a patient's status from an awake state to 
an anesthesia state in which the patient can then be operated.  
Propofol is widely used clinically and has become the most commonly used IV anesthetic for the 
induction of general anesthesia in clinical practice due to its fast onset, rapid recovery, high 
clearance, and convenient target-controlled IV slow injection. However, when propofol is given 
at its traditional induction dose (2.0-2.5 mg/kg), a single loading dose can cause severe 
hemodynamic fluctuations, resulting in a significant drop in the patient’s blood pressure, i.e., 
15% to 40% [6,7], especially in elderly patients [8]. At the same time, the incidence of 
respiratory depression is also high when propofol is used; in addition, as the most common 
adverse reaction of propofol [5], injection-site pain increases the stress and anxiety of the patient, 
directly or indirectly affecting the smoothness of anesthesia induction. Due to these 
shortcomings, a new and more advantageous sedative/anesthetic is needed in the market. 
Pain on injection is one of the key indicators in clinical trials. Methods employed in many 
clinical practices to alleviate pain on propofol injection have demonstrated negligible effects [1]. 
Pain on propofol injection is widely believed to be related to the aqueous phase concentration of 
the drug in the lipid emulsion formulation [9].  
The active ingredient HSK3486 is a chemical entity similar to propofol. It is a single 
diastereomer with 2 R-type chiral centers; the strategy in the medicinal design of HSK3486 is to 
realize systematic improvement in the pharmacological and physicochemical properties of the 
drug-receptor binding, thereby resulting in a compound that is expected to be clinically superior 
to propofol. HSK3486 has the pharmacodynamic (PD) characteristics of fast onset and quick 
stable recovery. HSK3486 has better target selectivity and higher in vitro and in vivo activities 
than propofol, and its potency is approximately 4 to 5 times that of propofol; the free HSK3486 
concentration in water phase measured by "ultrafiltration" is lower than that of propofol (MCT 
Fresenius), suggesting that the injection pain of HSK3486 may be reduced or eliminated versus 
propofol. 

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The primary mechanism of action for HSK3486 is to enable chloride ion influx by enhancing the 
GABAA-mediated ion channel, so as to inhibit the central nervous system. This channel is also a 
major target of propofol action. 
As of Aug 2023, 27 Phase I to III clinical studies evaluating HSK3486 have been completed and 
3 are ongoing or planned. Among the 27 completed studies, a total of 1370 were enrolled in the 
HSK3486 group for observation (94 in Australia + 1108 in China + 168 in US), including 286 
healthy subjects (including 66 elderly), 16 subjects with mild to moderate liver damage, 20 
subjects with mild to moderate renal damage, 236 patients who underwent colonoscopy, 15 
subjects who underwent gastroscopy, 446 subjects who underwent elective surgery, 135 subjects 
who underwent bronchoscopy, and 126 subjects with mechanical ventilation in ICU, and 90 
subjects undergoing gynecology outpatient surgery. Results showed that HSK3486 is generally 
safe and well tolerated. HSK3486 featured quick onset, rapid pace of regaining consciousness, 
and approximately 4 to 5 times the potency of propofol. At the same time, compared with 
propofol the incidence of injection pain is extremely low, less respiratory depression, lower 
incidence of hypotension, and less impact on the circulatory system.  
HSK3486 was approved by the NMPA for "Sedation in Gastrointestinal Endoscopy" and 
"Induction of General Anesthesia" on December 11, 2020 and February 2, 2021, respectively. 
The NDA registration applications for the indications of "sedation for bronchoscopy diagnosis 
and treatment" and "general anesthesia induction and maintenance" were accepted by the NMPA 
on February, 2021 and June, 2021, respectively. 
For more details of clinical studies of HSK3486 conducted, please refer to the Investigator’s 
Brochure [10]. 
1.3. Benefit-risk Assessment 
To date, 27 completed Phase I to III clinical studies of HSK3486 have been carried out and a 
total of 1370 subjects have been exposed to HSK3486. Analysis of data from 1370 subjects have 
been completed. HSK3486 was generally safe and well tolerated. HSK3486 had better 
performance than propofol, as manifested by the following: the types of adverse reactions were 
generally comparable with those of propofol but with a slightly lower incidence; however, the 
incidence of injection-site pain was significantly lower than propofol, the incidence of dizziness 
and respiratory-related AEs as well as the proportion of subjects in need of airway management 
were lower than propofol, the incidence and extent of AEs of cardiovascular depression were 
comparable to those of propofol. 
HSK3486 showed rapid onset of general anesthesia, less airway reactivity during endotracheal 
intubation (e.g., coughing), relatively stable vital signs, time to successful induction comparable 
to or slightly better than that of propofol (refer to Propofol US PI 2017), and potency 

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approximately 4 to 5 times than that of propofol in 156 enrolled subjects undergoing an elective 
surgery; it meets the needs of general anesthesia induction during surgery well.  
To date, HSK3486 has been shown to be a safe and effective sedative/anesthetic in prior clinical 
trials that is suitable for induction of general anesthesia.  
Phase III studies in the US will be conducted to further evaluate the efficacy and safety of 
HSK3486. In these Phase 3 studies, study drug will be administered preoperatively in a 
controlled clinical setting by healthcare providers who experience in general anesthesia. Changes 
in vital signs will be closely monitored during anesthesia, and corresponding risk preventions 
and control measures will be implemented for known drug reactions such as hypotension and 
sinus bradycardia, so as to ensure the safety of subjects in clinical studies. An independent data 
monitoring committee (DMC) will be involved in the conduct of this study to maintain the safety 
of all subjects dosed with the study drug. 
In summary, the safety profile of propofol has been well described based on its clinical use over 
the past several decades. In 27 clinical studies thus far, HSK3486 has demonstrated a safety 
profile comparable to propofol, with potentially less cardiopulmonary instability, coupled with 
the additional benefit of less injection-site pain. The favorable benefit-risk assessment supports 
the continued evaluation of HSK3486 for induction of general anesthesia. 
More detailed information about the known and expected benefits and risks and reasonably 
expected AEs of HSK3486 may be found in the Investigator’s Brochure [10].  

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2. OBJECTIVES AND ENDPOINTS
2.1. Objectives
The primary objective of this study is: 
To demonstrate HSK3486 0.4/0.2 mg/kg (0.4 mg/kg IV slow injection for the first dose, an
additional 0.2 mg/kg dose if needed) is non-inferior to propofol 2.0/1.0 mg/kg (2.0 mg/kg IV
slow injection for first dose, an additional 1.0 mg/kg dose if needed) in success of induction
of general anesthesia in adults undergoing elective surgery.
The secondary objectives of this study are: 
Key secondary objectives: 
 To confirm that HSK3486 0.4/0.2 mg/kg leads to statistically significant less injection-site
pain in all compared to propofol 2.0/1.0 mg/kg during the induction of general anesthesia in
adults undergoing elective surgery.
 To demonstrate HSK3486 0.4/0.2 mg/kg provides better anesthetic effects compared to
propofol 2.0/1.0 mg/kg without significant cardiac and respiratory depression in conjunction
with other routinely used preinduction and maintenance anesthetic agents in the induction of
general anesthesia in adults undergoing elective surgery.
Additional Efficacy Objective: 
 To evaluate HSK3486 0.4/0.2 mg/kg induction time in general anesthesia compared to
propofol.
 To evaluate HSK3486 0.4/0.2 mg/kg time to the disappearance of eyelash reflex compared
to propofol.
To confirm that HSK3486 0.4/0.2 mg/kg leads to statistically significant less moderate
injection-site pain compared to propofol 2.0/1.0 mg/kg during the induction of general
anesthesia in adults undergoing elective surgery.
 To better characterize that HSK3486 0.4/0.2 mg/kg leads to statistically significant less
average NRS scales in injection-site pain compared to propofol 2.0/1.0 mg/kg during the
induction of general anesthesia in adults undergoing elective surgery.
Safety: 
 To evaluate the overall safety profile of HSK3486 compared to propofol.
Pharmacokinetic: 
 To characterize the HSK3486 population pharmacokinetic (PK) profile.

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2.2. Endpoints
The primary efficacy endpoint is: 
 Success rate of general anesthesia induction: A successful general anesthesia induction will
meet both of the following conditions:
a)
b) One or less top-up dose required without using any rescue drugs.
The secondary efficacy endpoints are: 
Key secondary endpoints: 
 The proportion of subjects with any injection-site pain at time of drug administration on the
Numeric Rating Scale (NRS 1).
 The proportion of subjects with successful induction who maintain the desired depth of
anesthesia for general elective surgery, and without significant cardiac and respiratory
depression within15 minutes post initiation start of study drug administration, or up to the
start of second tracheal intubation attempt if it is a difficult condition and not beyond 15
minutes post initiation study drug administration, defined by all the following conditions:
a) Desired depth of anesthesia for general elective surgery is defined if all following
criteria are met:
i)
No clinical signs of inadequate depth of anesthesia, such as lacrimation, movement,
vomiting, coughing, laryngospasm, bucking, swallowing reflex or bronchospasm etc.
ii) No blood pressure (SBP, DBP, or MAP) increases more than 20% from baseline in
response to any major operational procedures or noxious stimulus in defined period.
iii) Subjects maintain desired depth of anesthesia for general elective surgery with BIS as
an objective assessment (after reaching initial lowest value, BIS remains sustainable
level at not more than 60).
Note: Target BIS 40-60 for general anesthesia (Appendix 2).
The BIS sustainable level at not more than 60 is defined as not more than 1 episode 
with BIS value observed >60 after reaching initial lowest value, in defined period. 
Note: It is strongly recommended that each site have available video laryngoscopy, or an 
acceptable alternative, for unanticipated difficult tracheal intubation. If more than one 
tracheal intubation attempt is made, the start and end times of first and subsequent 
intubation attempts should be recorded. 

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b) No significant respiratory depression, such as apnea, prior to the administration of
rocuronium bromide.
Note: For the composite endpoint, respiratory depression includes apnea, defined as
absence of thoracic movement lasting >30 seconds, or hypoxia, defined as oxygen
saturation (SpO2) <90% lasting >30 seconds, or life-threatening respiratory
depression requiring immediate intervention.
c) No significant cardiac depression indicated by BP decrease that requires intervention,
i.e., vasopressors and/or IV fluid resuscitation.
Note: For the composite endpoint, cardiac depression is defined as SBP <90 mmHg 
lasting >2 minutes plus requiring medical intervention such as inotropes, 
vasopressors, or IV fluid resuscitation, or life-threatening hypotension requiring 
immediate intervention.
Other secondary endpoints: 
 Time to successful induction of general anesthesia: Time from the end of the first
 Time to the disappearance of eyelash reflex: Time from the end of the first administration of
the study drug to the disappearance of eyelash reflex.
 The proportion of the subjects with any moderate injection-site pain at time of drug
 The average injection site pain at time of drug administration of HSK3486 compared to
propofol on NRS.
The safety endpoints are: 
 Safety assessments include AEs, adverse events of special interest (AESI), clinical
laboratory test results, vital signs (supine HR, SBP and DBP, MAP, respiratory rate [RR],
and SpO2), electrocardiogram (ECG) findings, physical examination findings, and
administration of additional medications, or any interventions including medical
interventions, e.g., administration of vasoactive drugs to treat clinically relevant changes in
BP.
The PK endpoint is: 
 Plasma HSK3486 concentration at scheduled time points (Appendix 5).

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3. INVESTIGATION PLAN
3.1. Overall Study Design and Plan Description
This is a global multicenter, randomized, double-blinded, propofol-controlled, Phase 3 clinical 
study to evaluate the efficacy and safety of HSK3486 for induction of general anesthesia in 
adults undergoing elective surgery with endotracheal intubation.  
After screening, eligible subjects will be randomized in a 2:1 ratio to receive either HSK3486 
0.4/0.2 mg/kg (i.e., 0.4 mg/kg IV slow injection over 30 [±5] seconds followed by an additional 
0.2 mg/kg dose if needed) or propofol 2.0/1.0 mg/kg (i.e., 2.0 mg/kg IV slow injection over 30 
[±5] seconds followed by an additional 1.0 mg/kg dose if needed) in a blinded manner. Enrolled 
subjects will be stratified by American Society of Anesthesiologist Physical Status (ASA-PS; I-II 
and III-IV), age (<65 and >65 years), and Body Mass Index (BMI<35 and >35 kg/m2). 
Endotracheal intubation will be performed after adequate anesthetic induction (Modified 
Appendix 1) has been achieved 
and administration of neuromuscular blocking agent. 
On Day 1, premedication is allowed prior to induction except for sedative hypnotics, analgesics 
(e.g., opioids, NSAIDS, APAP), or any medications that relieve pain (e.g., Gabapentin), unless 
otherwise specified in the protocol. Premedication should be recorded if used. (See Section 6.2.) 
Assessments and procedures completed prior to the administration of the study drug in the 
operating room, the preinduction period, induction period, maintenance period and the follow-up 
period can be found in Section 7 – Study Assessments and Procedures. 
Population PK Study 
All eligible subjects in this clinical study will be required to provide venous blood samples for 
population PK study per time schedule planned (Appendix 5). 
3.2. Discussion of Study Design, Including the Choice of Control Groups 
Propofol is selected as the rescue/alternative drug (trade name: Diprivan). Given that propofol is 
the most common IV drug for anesthesia, should induction fail, the investigator, based on the 
status of the subject, should give propofol as the first choice of rescue medication; however, 
based on the discretion of the investigator, an alternative drug may be administered at the 
appropriate dose and timing as determined by the investigator. 
The population in this study includes subjects undergoing elective surgeries that require 
endotracheal intubation under general anesthesia. For safety considerations, subjects undergoing 
emergency, cardiothoracic, and intracranial surgeries are excluded. 

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3.3. End of Study Definition 
The end of the study is defined as the date of the last visit of the last subject in the study. 
3.4. Selection of Doses in the Study
3.4.1. 
Selection of Dose for HSK3486 
By analyzing the results of the dose-ascending group and the extension stage (primary) in the 
Phase II study of HSK3486 for induction of general anesthesia, the following can be seen that: 
Dose selection: Although the efficacy of the 0.5mg/kg HSK3486 group was comparable to
that of the 2.0mg/kg propofol group, the efficacy of the 0.5mg/kg HSK3486 group was
slightly better than that of the 0.3mg/kg and 0.4mg/kg HSK3486 groups, as manifested by a
faster rate of successful induction and a shorter intubation time. In addition, the 0.5mg/kg
HSK3486 group did not need top-up doses. However, the safety of the 0.5mg/kg HSK3486
group was slightly worse, as manifested by a larger decrease in BP, higher incidence of
injection pain than 0.3 mg/kg HSK3486, and more cases of transient and prolonged QT
interval. The 0.3mg/kg HSK3486 group showed better safety and a relatively small
proportion of adverse drug reactions (ADRs). However, compared with the propofol group,
the 0.3mg/kg group had longer time to successful induction, more obvious intubation
reactions, and larger fluctuations in BP and HR, suggesting that the inhibitory effect of
0.3 mg/kg HSK3486 on intubation reactions is not strong enough. At a suitable dose,
HSK3486 can be used to induce general anesthesia with a reliable efficacy and safety
profile. Based on above considerations, it is reasonable to choose the dose of 0.4 mg/kg
(initial dose)/0.2 mg/kg (top-up dose) as the recommended HSK3486 dose for Phase III
clinical studies.
Number of top-up doses and dosing interval: Based on the results of Phase II study for
induction of general anesthesia, during induction, 1 (1/8) subject in the dose-escalation
group (HSK3486 0.4/0.2mg/kg group) received 1 top-up dose of study drug, and 1 (1/22)
subject in the dose-extension stage (0.3/0.15mg/kg group) received 1 top-up dose of study
drug. The time to successful induction was about 0.71 to 1 minute. Therefore, in the design
of Phase III study on induction of general anesthesia, the top-up dose is set at 1 minute ±10
seconds after the initial dose of the study drug. If the number of top-up doses is more than 1,
the induction is considered to have failed.
 Criteria for the selection of top-up dose: The criteria for a top-up dose in the Phase III study
of induction of general anesthesia are based on the results of the previous Phase II and III
studies in China and are determined according to the MOAA/S score, 
not reached, a top-up dose is required.
 

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and propofol. 
will be automatically adjusted 
to a 25% dose reduction. According to the judgment of the investigator, the dose can be 
further reduced by up to 50%. For ASA grade 3-4 subjects, the dose can be reduced by 25-
50% per investigator discretion. The administration time should be extended to 1 minute for 
these populations. 
 Data from a PK study in elderly subjects (Study HSK3486-108) suggests that there are no
non-elderly (<65 years old), consistent with physiologically based PK modeling results. A
similar efficacy and safety profile was also demonstrated between 0.3 mg/kg elderly and 0.4
mg/kg non-elderly. Of note, both propofol US and UK labels currently recommend a 25%
dose reduction in subjects 65 or older.
kg/m2,
total body weight (TBW) will be used to determine dose; for subjects with BMI >40 kg/m2,
lean body weight (LBW) will be used to determine dose.
 Based on population PK (PopPK) modeling, subjects with BMI >45 kg/m2 can be expected
to have higher simulated maximum concentration (Cmax) and area-under-the-curve (AUC[0-24
h]) than the PK exposure identified in the Phase 1 single-ascending-dose studies.
 In obese subjects, fat mass and LBW do not increase proportionately. Obese subjects have
both an increased amount of fat and LBW; however, LBW accounts for as much as 20% to
40% of the excess TBW. The LBW has been shown to correlate with cardiac output, which
is an important determinant in the early distribution kinetics of drugs. Based on published
literature, LBW is the ideal weight scalar for drug administration in subjects with BMI >40
[11, 12].
 The anesthetic PD response in subjects with BMI >40 kg/m2is likely to be different. It has
been reported that morbidly obese subjects have significantly decreased half-maximal
effective concentration (EC50) values compared to control subjects, which suggests increased
brain sensitivity to propofol in the morbidly obese population [13, 14].
3.4.2. 
Selection of Dose for Propofol 
Propofol has the characteristics of fast onset, rapid recovery, high clearance, and convenient 
target-controlled slow injection. It has become the most commonly used IV anesthetic in clinical 
practice. Among various propofol products, Diprivan is the most widely used both domestically 
and overseas; therefore, propofol is selected as the positive control drug.  
Rationale for the selection of the initial and top-up doses of propofol: 
In the packaging insert of propofol [9], the dose for inducing general anesthesia is 2.0 to 2.5 
mg/kg. The results of the dose-ascending and extension stages of Phase II trials on induction of 
general anesthesia by HSK3486 showed the propofol 2.0 to 2.5mg/kg group had a 100% success 

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rate in induction of general anesthesia with good safety and tolerability. Among them, 2.5 mg/kg 
was found to have a higher incidence of ADRs during dose escalation, so 2.0 mg/kg was selected 
as the control dose in the extension stage; in summary, to better compare the differences in the 
efficacy and safety of HSK3486 and propofol, 2.0 mg/kg of propofol has been selected as the 
initial dose, and the top up- dose is the same as that in the design of the Phase II study on 
induction of general anesthesia, i.e., 1.0 mg/kg. 
The propofol dose will be modified for obese subjects to match HSK3486 dosing regimens due 
kg/m2, TBW will be 
used to determine dose; for subjects with BMI >40 kg/m2, LBW will be used to determine dose. 
3.5. Data Monitoring Committee 
An independent DMC will be involved in the conduct of the study to ensure the safety and well-
being of all subjects who have been administered study drug is maintained. The DMC will 
consist of 3 unblinded members: 2 anesthesiologists with appropriate clinical expertise and 1 
statistician. The DMC will review all available unblinded safety information at approximately 
30% enrollment. Additionally, enrollment will be immediately suspended after one death during 
the study where a clear alternative cause is not readily apparent (i.e., deemed definitely or likely 
related to study drug); after 4 (4/399 [1%]) non-fatal serious adverse events (SAEs) where a clear 
alternative cause is not readily apparent (i.e., deemed definitely or likely related to study drug); 
or after 8 (8/399 [2%]) severe AEs of special interest (AESIs; Common Terminology Criteria 
[CTCAE] Grade 3 and 4) of severe hypotension, bradycardia, hypoxia due to respiratory 
depression, or QTc prolongation occurring within 15 minutes of study drug administration, 
where a clear alternative cause is not readily apparent (i.e., deemed definitely or likely related to 
met, enrollment will be temporarily suspended while the DMC convenes to review all available 
unblinded data. Based on recommendations from the DMC, the Sponsor can terminate the study 
or resume enrollment if measures can be taken to effectively address (i.e., mitigate) the risk to 
safely continue the study, such as revising inclusion/exclusion criteria or study procedures. The 
final decision to suspend enrollment or proceed will be made by the Sponsor after consultation 
with the DMC members and taking into consideration their recommendations. Conduct of the 
DMC is described in the DMC Charter.  

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4. SELECTION OF STUDY POPULATION
Prospective approval of protocol deviations to recruitment and enrollment criteria, also known as 
protocol waivers or exemptions, is not permitted. 
4.1. Inclusion Criteria 
Subjects must satisfy all of the following criteria at the screening and Day 1 visits: 
1. Subjects undergoing elective surgery (non-emergency, non-cardiothoracic, and non-
intracranial surgery anticipated to last at least 1 hour) requiring endotracheal intubation
and inhalation general anesthesia during the maintenance period. Duration of surgery is
defined as time from study drug administration to time of transfer from operating room to
recovery room or PACU.
2.
-PS I to IV (Appendix 6). For ASA-PS
IV subjects, clinical status must be optimized at time of preoperative anesthesia
evaluation per judgement of the anesthesiologist.
3.
2.
4.
2 
2% in ambient air; SBP
 
5. For all women of childbearing potential, negative serum pregnancy test within the
screening period and negative urine pregnancy test at baseline (Day 1). Additionally,
women of childbearing potential* and male subjects with female partners of childbearing
potential must agree to use contraception as defined in 7.3.4 from the time of consent
until 30 days post study drug administration.
6.
For subjects with known hypothyroidism and/or on thyroid-hormone replacement
treatment (i.e., thyroxine), or subjects suspected to have thyroid dysfunction based on
clinical laboratory and physical exam, a TSH must drawn and be within normal levels.
7. Capable of understanding the procedures and methods of this study, willing to sign an
Informed Consent Form (ICF), and able to complete this study in strict compliance with
the study protocol.
8. Willing to comply with the site’s COVID guidelines and testing requirements as
applicable.
9.
Patients with psychiatric/mental disorders must be considered stable on treatment (e.g.,
SSRIs, SNRIs, TCAs, MAOIs, psychotherapy) per investigator judgement, and no
hospitalization and urgent care due to the underlying psychiatric pathology for at least 12
months.
** Women are considered of childbearing potential until becoming post-menopausal, unless she 

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had a documented hysterectomy or bilateral oophorectomy / salpingo-oophorectomy. A woman 
is considered to be post-menopausal if she had no menses for at least 12 consecutive months 
(without an alternative medical cause).
4.2. Exclusion Criteria
Subjects will be excluded from the study if they satisfy any of the following criteria at the 
screening and Day 1 visits: 
1. Contraindications to deep sedation/general anesthesia or a history of adverse reaction to
sedation/general anesthesia.
2. Known to be allergic to eggs, soy products, opioids and their antidotes, or propofol;
subjects having contraindications to propofol, opioids, and their antidotes. In cases where
the only previous reaction to opioids was itching or nausea, subjects need not be excluded
if the investigator believes the subject is not truly allergic to opioids.
3. Medical condition or evidence of increased sedation/general anesthesia risk as follows:
a) Cardiovascular disorder: uncontrolled hypertension (SBP >160 mmHg and/or DBP
>100 mmHg) with or without antihypertensive therapy (antihypertensive therapy
should be stable for 1 month prior to screening), serious arrhythmia (including the
subjects with implanted pace makers), unstable heart failure, Adams-Stokes
syndrome (i.e., syncope or near-syncope due to cardiac arrythmia), unstable angina,
myocardial infarction occurring within 6 months prior to screening, history of
tachycardia/bradycardia requiring medications, third-degree atrioventricular block or
for males
ms for females.
b)
History of severe obstructive lung disease (i.e., forced expiratory volume in 1
second [FEV1] <50% predicted), history of bronchospasm requiring treatment in a
hospital emergency room or hospitalization occurring within 3 months prior to
screening, developing acute respiratory tract infection within 2 weeks prior to
baseline (such as symptoms of fever, shortness of breath, wheezing, nasal congestion,
and cough).
c) Cerebrovascular disease: subject with a history of serious craniocerebral injury,
convulsion, seizure disorder, intracranial hypertension, cerebral aneurysm, or stroke.
d) Patients with psychiatric/mental disorders who have not been on a stable treatment
regimen (e.g., SSRIs, SNRIs, TCAs, MAOIs, psychotherapy) per investigator
judgement, for at least 12 months or who have been hospitalized or had
emergent/urgent care due to the underlying psychiatric pathology within the last 12
months.

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e) Uncontrolled clinically significant conditions of liver (e.g., severe hepatic
insufficiency defined as Childs-Pugh class C), kidney, gastrointestinal tract, blood
system, nervous system, or metabolic system diseases, judged by the investigator to
be unsuitable for involvement in the study.
f)
History of uncontrolled diabetes in the opinion of the investigator.
g) History of alcohol abuse within 3 months prior to screening, where alcohol abuse
refers to daily alcohol drinking >2 units of alcohol (1 unit = 360 mL of beer or 45 mL
of spirit with a strength of 40% or 150 mL of wine).
h) History of drug abuse that, in the opinion of the investigator, may confound the
interpretation of safety or efficacy in a study subject.
i)
For subjects with known hypothyroidism and/or on thyroid-hormone replacement
treatment (i.e., thyroxine), or subjects suspected to have thyroid dysfunction based on
clinical laboratory and physical exam who has a TSH value outside the normal range.
4. Management risks of respiratory tract and judged by the investigator to be unsuitable for
inclusion in the study as follows:
a) Asthma must be stable: stable doses of asthma medications for the past 6 months, no
requirement for rescue inhalers or oral steroids within past 6 months, not evaluated in
emergency department, urgent care, or hospitalized for an asthma attack within past 1
year.
b) History (or family history) of malignant hyperthermia.
c) Any previous failure of tracheal intubation.
d) Judged to have a difficult airway for endotracheal intubation in the opinion of the
Investigator based on parameters such as modified Mallampati score (Grade III or IV
[Appendix 7]), neck mobility, short thyromental distance, and/or history of difficult
intubation).
5. Any medication that has the potential to interact synergistically with propofol or
HSK3486, including but not limited to all sedatives and hypnotics (e.g., benzodiazepines
and opioids), taken within 5 half-lives prior to Day 1.
6. Laboratory parameters measured at screening with the following levels:
a)
Neutrophil count 1.5 x 109/L
b)
Platelet count <80 x 109/L
c)
Hemoglobin <90 g/L (without blood transfusion within 14 days)
d)
Alanine transaminase and/or aspartate transaminase 2.0 x upper limit of normal
(ULN)
e)
Total bilirubin 2.0 x ULN
f)
Severe renal impairment defined by creatinine clearance (CrCl) 30 mL/min

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7. Female subjects with a positive pregnancy test at screening (serum) or baseline (urine);
lactating subjects; any subject planning to get pregnant within 1 month after the study
(including the male subject’s partner).
8.
Judged by the investigator to have any other factors that make the subject unsuitable for
participation in the study.
4.3. Subject Discontinuation from the Study
Subject discontinuation from the study criteria will include but is not limited to the following: 
1. Withdrawal of informed consent;
2. If it is believed that continued participation in the study will be detrimental to the
subject’s health as determined by the investigators and Sponsor's medical personnel; or
3. Other.
Subjects may withdraw from the study at any time at his/her own request or may be withdrawn at 
any time at the discretion of the investigator for safety, behavioral, or administrative reasons. 
Subjects should complete pre-withdrawal examinations as much as possible (same as the 
follow-up examinations) prior to withdrawal. For subjects who prematurely withdraw from the 
study, the investigator should ask for the reasons for withdrawal and record in the original 
documents and electronic Case Report Form (eCRF), and complete the test items for the end of 
study visit. During the study, if it is found that a female subject or the sexual partner of a male 
subject is pregnant, the investigator must immediately report it to the Sponsor and the Ethics 
Committee (EC). All relevant information must be recorded in the source file and the eCRF. 
4.3.1. 
Screen Failures 
Screen failures are defined as subjects who consent to participate in the clinical study but are not 
subsequently randomized. A minimal set of screen failure information is required to ensure 
transparent reporting of screen failure subjects to meet the Consolidated Standards of Reporting 
Trials publishing requirements and to respond to queries from regulatory authorities. Minimal 
information includes demography, screen failure details, eligibility criteria, and any SAEs. 
Subjects who do not meet the criteria for participation in this study may be rescreened once 
within 60 days. Re-screening must be approved by the medical monitor if the screen fail reason 
is anything other than the screening window has elapsed. Rescreened subjects should be assigned 
the same subject number as for the initial screening. 
4.3.2. 
Subject Discontinuation from Study Treatment 
If a subject experiences an AESI of severe hypotension, bradycardia hypoxia due to respiratory 
depression, QTc prolongation, cardiac arrythmia, or allergy/anaphylaxis which persists despite 

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routine interventions and 
requiring a top-up dose, the subject should stop study treatment and not receive the top-up dose. 
It is up to the discretion of the Investigator and surgeon whether to proceed with the surgery 
using alternative standard-of-care medications to induce general anesthesia. Subjects who stop 
study treatment will remain in the study and continue to be followed for safety assessments 
according to the Schedule of Assessments.  
If the subject withdraws consent for disclosure of future information, the Sponsor may retain and 
continue to use any data collected before such a withdrawal of consent. Additionally, subjects 
may request destruction of any samples taken and not tested, and the investigator must document 
this in the site study records. Refer to the Schedule of Assessments for data to be collected at the 
time of study discontinuation and follow-up and for any further evaluations that need to be 
completed.
Additional reasons for study treatment discontinuation can include, but are not limited to: 
 Death
 Adverse event
 Sponsor determination.
 Investigator discretion
4.3.3. 
Lost to Follow-up
A subject will be considered lost to follow-up if he/she repeatedly fails to complete scheduled 
visits and is unable to be contacted by the study site.  
The following actions must be taken if a subject fails to complete required study visits or 
declines a phone contact by study site: 
 The site must attempt to contact the subject and reschedule the missed visit as soon as
possible, counsel the subject on the importance of maintaining the assigned visit schedule,
and ascertain whether or not the subject wishes to and/or should continue in the study.
 In cases in which the subject is deemed lost to follow-up, the investigator or designee must
make every effort to regain contact with the subject (where possible, 3 telephone calls and, if
necessary, a certified letter to the subject’s last known mailing address or local equivalent
methods). These contact attempts should be documented in the subject’s medical record.
 Should the subject continue to be unreachable, he/she will be considered to have withdrawn
from the study with a primary reason of lost to follow-up.

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4.3.4. 
Replacement Procedures 
Subjects who discontinue or withdraw from the study will not be replaced. 
4.3.5.
Follow-up of Subjects Prematurely Discontinued from the Study Treatment 
Regimen or Withdrawn from Study 
Subjects who discontinue from the study will be asked to complete final study procedures and 
will be contacted for the Follow-up Telephone Call as described in the Schedule of Assessments. 
All SAEs that are ongoing at the time of discontinuation, or that develop prior to the final 
Follow-up Telephone Call, will be followed for 30 days, or until resolution or stabilization. 
4.4. Study Stopping Criteria 
Enrollment will be immediately stopped after one death where a clear alternative cause is not 
readily apparent (i.e., deemed definitely or likely related to study drug); after 4 (4/399 [1%]) 
non-fatal serious adverse events (SAEs) where a clear alternative cause is not readily apparent 
(i.e., deemed definitely or likely related to study drug); or after 8(8/399 [2%]) severe AEs of 
special interest (AESIs; Common Terminology Criteria for Adverse Events [CTCAE] Grade 3, 4 
or 5) of severe hypotension, bradycardia, hypoxia due to respiratory depression, or QTc 
prolongation occurring within 15 minutes of study drug administration, where a clear alternative 
cause is not readily apparent (i.e., deemed definitely or likely related to study drug), and lasting 
re met, enrollment will be 
temporarily suspended while the DMC convenes to review all available unblinded data. Based on 
recommendations from the DMC, the Sponsor can terminate the study or resume enrollment if 
measures can be taken to effectively address (i.e. mitigate) the risk to safely continue the study, 
such as revising inclusion/exclusion criteria or study procedures. The final decision to suspend 
enrollment or proceed will be made by the Sponsor after consultation with the DMC members 
and taking into consideration their recommendations. Conduct of the DMC is described in the 
DMC Charter. 
The determination of thresholds to suspend enrollment incorporates safety data from the prior 
Phase 3 study in China (Study HSK3486-302) evaluating HSK3486 compared to propofol for 
induction of general anesthesia in subjects undergoing elective surgery. In that study, 1 (1/176 
[0.6%]) propofol-treated subject experienced an SAE of bronchospasm (deemed unlikely related 
to study drug), and 2 (2/176 [1%]) subjects experienced severe AESIs of hypotension (deemed 
related to study drug; 1 HSK3486-treated subject and 1 propofol-treated subject). However, the 
prior Phase 3 study in China enrolled a healthier subject population, in that only ASA-PS class I 
to II subjects, no elderly subjects (age >65), and no obese subjects (BMI>30) were included.  
Inclusion criteria for the current study includes ASA-PS class III to IV subjects, elderly subjects 
(age >65 years with no upper limit), and obese subjects (BMI >30 with no upper limit). It is 
anticipated this study will enroll a less healthy subject population with more comorbidities, 

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therefore, thresholds to suspend enrollment of 4 (4/399 [1%]) non-fatal SAEs related to study 
drug and 8 (8/399 [2%]) severe AEs of interest (CTCAE grade 3and 4) of severe hypotension, 
bradycardia, hypoxia due to respiratory depression or QTc prolongation occurring within 15 
minutes of study drug administration or allergy/anaphylaxis have been selected using clinical 
judgement. 
The Sponsor reserves the right to close a study site or terminate the study at any time for any 
reason at the sole discretion of the Sponsor. Study sites will be closed upon study completion. A 
study site is considered closed when all required documents and study supplies have been 
collected and a study site closure visit has been performed.  
Reasons for study termination may include, but are not limited to: 
Adverse events unknown to date (i.e., not previously reported in any similar investigational
study drug trial with respect to their nature, severity, and/or duration);
 Increased frequency and/or severity and/or duration of known, anticipated, or previously
reported AEs (this may also apply to AEs defined at check-in as baseline signs and
symptoms);
 Medical or ethical reasons affecting the continued performance of the study;
 Difficulties in the recruitment of subjects;
 Cancellation of drug development.
The investigator may initiate study site closure at any time, provided there is reasonable cause 
and sufficient notice is given in advance of the intended termination.  
Reasons for the early closure of a study site by the Sponsor or investigator may include, but are 
not limited to:
Failure of the investigator to comply with the protocol, the requirements of the Institutional
Review Board (IRB)/EC or local health authorities, the Sponsor's procedures, or GCP
guidelines.
 Inadequate recruitment of participants by the investigator.

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5. STUDY TREATMENTS
5.1. Treatments Administered
Investigational drug: HSK3486 injectable emulsion  
Strength: 50 mg HSK3486 per 20 mL  
Supplier: Haisco-USA Pharmaceuticals, Inc.
Control drug: Propofol injection  
Trade name: Diprivan 
Strength: 200 mg Propofol per 20 mL 
Supplier: Haisco-USA Pharmaceuticals, Inc.
5.2. Preparation, Storage, Handling, and Accountability 
HSK3486 
 Propofol 
-
F) and should not be frozen. Both HSK3486 
and propofol should be shaken well before use. The investigator or designee must confirm 
appropriate temperature conditions have been maintained during transit for all study treatment 
received and any discrepancies are reported and resolved before use of the study treatment. All 
study treatments must be stored in a secure, environmentally controlled, and monitored (manual 
or automated) area in accordance with the labeled storage conditions with access limited to the 
investigator and authorized site staff. 
The Sponsor will provide sufficient amounts of the study drugs during the study. After the 
Sponsor provides the study drug to the study site, the principal investigator or authorized 
representative will sign for receipt upon verification and record information of the study drugs. 
Once receiving the study drugs, the study site should store it under the storage conditions 
specified in the drug label. The study drugs should be kept by a designated personnel 
(non-blinded) authorized by the investigator for regular accounting and record of the drug 
quantity. Non-blinded clinical research associates (CRAs) will supervise the drug management.
Only subjects enrolled in the study may receive study treatment and only trained and authorized 
site staff may supply or administer study treatment. The investigator, institution, or the head of 
the medical institution (where applicable) is responsible for study treatment accountability, 
reconciliation, and record maintenance (i.e., receipt, reconciliation, and final disposition 
records). The study drugs cannot be used for any purpose other than this clinical study, and it 
may not be sold or transferred to anyone who is not involved in this clinical study. 

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The use of each study drug should be recorded in a timely manner with a signature upon review. 
All remaining drug products, packaging, and labels are returned or destroyed at the sites after 
Sponsor authorization during and after the study. Remaining empty vials and packages are 
destroyed and recorded according to the standard operating procedures at local study sites. 
Further guidance and information for the final disposition of unused study treatment are provided 
in the pharmacy manual.
Drug Preparation 
On the day of drug administration, the non-blinded preparation personnel will locate the 
corresponding study drug according to the drug number applied by the Interactive Web Response 
System (IXRS) and convert the drug volume according to formula below; the drug will then be 
drawn into a certain number of syringes (1 initial dose + 1 top-up dose). In addition, the required 
alternative (propofol) rescue drug for the subjects is also prepared. 
The non-blinded administration personnel will check the drug information recorded by the 
preparation staff and transfer the study drug to the operating room. Both the drug volume 
calculation and drug preparation processes should be documented in the drug dispensing record 
form.
Staff for drug preparation and administration are non-blinded and must not disclose subject 
administration information to investigators, subjects, or any other blind personnel. 
Doses and method of administration for HSK3486 are as below: 
Volume of initial/top-up dose = dose group (mg/kg) x baseline body weight (kg) ÷ 2.5 mg/mL. 
HSK3486 is administered by using 30 [±5] seconds of slow injection. If a top-up dose is 
required, 50% of the initial calculated dose is given via slow injection over 10 [±2] seconds. 
, the dose will be automatically adjusted to a 25% dose 
reduction. According to the judgment of the investigator, the dose can be further reduced by up 
to 50% of the calculated dose. The administration time should be extended up to 1 minute for 
this population (IV slow injection over 30-60 seconds).  
For ASA grade 3-4 subjects, the dose can be reduced by 25-50% per investigator discretion. The 
administration time should be extended up to 1 minute for these populations (IV slow injection 
over 30-60 seconds). 

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2, TBW will be used to determine HSK3486 dose; for subjects 
with BMI >40 kg/m2, LBW will be used to determine HSK3486 dose, and Rescue dose. LBW 
should be calculated using the Janmahasatian formula included below. 
The Janmahasatian Formula: 
•
For males: LBW = (9270 x TBW (kg)) / (6680 + (216 x BMI))
•
For females: LBW = (9270 x TBW (kg)) / (8780 + (244 x BMI))
Doses and method of administration for propofol are as below: 
Volume of initial/top-up dose of propofol = dose group (mg/kg) x baseline body weight (kg) ÷ 
10 mg/mL. The method of administration of propofol is the same as that of HSK3486. 
, the dose will be automatically adjusted to a 25% dose 
reduction. According to the judgment of the investigator, the dose can be further reduced by up 
to 50% of the calculated dose. The administration time should be extended up to 1 minute for 
this population (IV slow injection over 30-60 seconds).  
For ASA grade 3-4 subjects, the dose can be reduced by 25-50% per investigator discretion. The 
administration time should be extended up to 1 minute for these populations (IV slow injection 
over 30-60 seconds). 
kg/m2, TBW will be used to determine propofol dose; for subjects 
with BMI >40 kg/m2, LBW will be used to determine propofol dose, and Rescue dose. LBW 
should be calculated using the Janmahasatian formula included below. 
The Janmahasatian Formula: 
•
For males: LBW = (9270 x TBW (kg)) / (6680 + (216 x BMI))
•
For females: LBW = (9270 x TBW (kg)) / (8780 + (244 x BMI))
The rescue dose, propofol, is prepared as 100% of the initial calculated dose and administered 
per propofol guidelines above. The rescue dose administration time should be extended up to 1 
minute (IV slow injection over 30-60 seconds) for 
65 years of age and for ASA 
grade 3-4 subjects who require a dose reduction. 
5.3. Method of Treatment Assignment 
The randomization of the subjects in this study is completed using the IXRS. After the subjects 
pass the screening, the investigator(s) or designee shall log into the IXRS and enter the general 

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information of each subject, such as the screening number, sex, age, height, and body weight, to 
obtain the randomization number. Then, the randomization number and corresponding treatment 
group are assigned using the IXRS and submitted to the drug preparation personnel (non-
blinded) for drug preparation. The non-blinded site staff will have to log into the IXRS to obtain 
the kit numbers and dosages. 
Study treatment will be dispensed at baseline visit (Day 1) summarized in the Schedule of 
Assessments.  
5.3.1. 
Dose Modification 
Dosage adjustments are not allowed unless these conditions are stipulated in the protocol for 
e
will be automatically adjusted to a 25% dose 
reduction. According to the judgment of the investigator, the dose can be further reduced by up 
to 50% of the calculated dose. For ASA grade 3-4 subjects, the dose can be reduced by 25-50% 
per investigator discretion. The administration time should be extended up to 1 minute for these 
populations (IV slow injection over 30-60 seconds). 
Both the HSK3486 and the propofol doses will be modified for morbidly obese subjects: For 
2, TBW will be used to determine dose; for subjects with BMI >40 
kg/m2, LBW will be used to determine dose. LBW should be calculated using the Janmahasatian 
formula included in section 5.2. 
5.4. Blinding
This is a double-blind study. The IXRS will be programmed with blind-breaking instructions. 
The study blind may be broken if, in the opinion of the investigator, it is in the subject’s best 
interest to know the study treatment assignment. Sponsor must be notified before the blind is 
broken unless identification of the study treatment is required for a medical emergency in which 
the knowledge of the specific blinded study treatment will affect the immediate management of 
the subject’s condition (e.g., antidote is available). In this case, Sponsor must be notified within 
24 hours after breaking the blind. The date and reason that the blind was broken must be 
recorded in the source documentation and eCRF, as applicable. 
The study staff are divided into 2 groups: blinded and non-blinded. The study ensures that the 
investigators, subjects, and the entire operating process of the study are blinded. 
Blinded staff include the principal investigator, study nurse, clinical research coordinator, 
blinded CRA, and all the other personnel involved in the documentation, assessment, analysis, 
and review of efficacy/safety endpoint data. These staff are primarily responsible for all study 

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procedures other than drug management (such as subject screening, documentation, assessment, 
analysis, and review of efficacy and safety endpoints). 
The non-blinded staff are responsible for the management, preparation, and administration of the 
study drug. Detailed descriptions of blinding procedures are described in the Blinding Plan. 
Once the study is started, both the blinded and non-blinded study staff ensure that their 
respective responsibilities are performed in strict accordance with protocol requirements. The 
blinded and non-blinded staff ensure that no communication related to study drug groups takes 
place to avoid unintentional unblinding. Once assigned, blinded and non-blinded staff must 
remain in their respective role for the duration of study participation.  
5.5. Treatment Compliance 
All study drugs will be administered at the site by site personnel; therefore, subject compliance 
will not be measured. 
5.6.  Preinduction Drugs 
Fentanyl and Midazolam 
All subjects will receive preinduction IV midazolam at dose 0.04 mg/kg, up to 3 mg maximum 
per subject over 15 [±2] seconds at 5 minutes [±30 seconds] prior to initiating study drug. And 
all subjects will receive preinduction IV fentanyl 1 mcg/kg rounded up to the nearest 25 mcg, up 
to 100 mcg maximum per subject over 15 [±2] seconds at 2 minutes [±10 seconds] prior to 
initiating study drug. Fentanyl and midazolam doses can be reduced according to the patient’s 
age and comorbidities as per the anesthetist’s discretion.  

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6. CONCOMITANT THERAPIES AND OTHER RESTRICTIONS
6.1. Concomitant Therapies
The investigator will review all medications and therapies taken by the subject (or are intended 
to be taken during the study) during screening and will assess whether or not the medications or 
therapies will impact the study. 
Any medication or vaccine (including over-the-counter or prescription medicines, vitamins, 
and/or herbal supplements) that the subject is receiving at the time of enrollment or receives 
during the study must be recorded along with: 
reason for use
dates of administration including start and end dates
 dosage information including dose and frequency.
The Medical Monitor should be contacted if there are any questions regarding concomitant or 
prior therapy. 
Any medications/supplements/vitamins used by the subjects during Day of Surgery and after 
surgery until the end of the study (Day 8) are classified as concomitant medication except for 
study drugs and combination drugs. 
Any medications/supplements/vitamins used before the Day of Surgery are classified as previous 
medications. All previous medications used by the subjects within 1 month before screening 
should be recorded in the source medical record and the eCRF. 
A record of concomitant medication should consist of drug name (generic name), dose and 
number of daily doses, method of administration, start and end time of administration, and 
reason for use.
Prior to, during, and after surgery, antiemetics, hemostatics and antibiotics can be used according 
to the clinical situation of the subjects. Postoperative use of analgesics can be determined by the 
investigators when needed. 
During the study, drugs that may affect the QT interval should be used with caution (Appendix 
8); in case of an emergency where such drugs must be used, please record the use of the drugs 
and the reason. 

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6.2. Prohibited Therapies 
No sedative hypnotics, anesthetic drugs, analgesics, systemic neuromuscular blocking agents or 
any medications that relieve pain other than those prescribed in the protocol are allowed on Day 
1 until the completion of endotracheal intubation, including but not limited to the following: 
 Inhalation anesthetics: isoflurane, enflurane, desflurane, halothane, anesthetic ether, nitrous
oxide
Note: Sevoflurane will be used for maintenance of general anesthesia upon the completion
of endotracheal intubation.
Intravenous anesthetics: thiopental sodium, ketamine, etomidate, sodium oxybate, lidocaine
 Systemic neuromuscular blocking agents (other than protocol-prescribed rocuronium):
atracurium, cisatracurium, mivacurium, succinylcholine, vecuronium, pancuronium
Note: Regional and local neuromuscular blocking agents and anesthetics are permitted if not
involving the upper extremity where study drug is administered.
 Analgesics : morphine, methadone, codeine, pentazocine, tramadol, NSAIDs, APAP, etc.
Note: Additional IV fentanyl may be administered for intraoperative analgesia only after
initiation of sevoflurane, preferably after 15 minutes following the end of study drug
administration.
 Other drugs that relieve pain: Gabapentin
 Benzodiazepines: Valium, Ativan, etc.
Note: spinal and epidural blocks are not permitted at any time on Day 1. 

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7. STUDY ASSESSMENTS AND PROCEDURES
7.1. Study Procedures 
7.1.1.
Screening Period (Day -13 to Day 1) 
During the screening period and before the investigator conducts any study procedures, he/she 
will discuss with subjects their clinical condition, the commonly used anesthesia methods, the 
characteristics of the study drug, and the possible adverse reactions that can be caused by the 
study drug. The subjects will sign the ICF and receive a signed copy.  
After signing the ICF, the subject will be assigned a screening number, and the following 
procedures/evaluations will be conducted as indicated in the Schedule of Assessments: 
 Assess inclusion/exclusion criteria.
 Demographics.
 Collect medical history (disease history, allergy history, alcohol history and surgical
anesthesia history, etc.).
 Prior medications.
 Modified Mallampati score assessment (Appendix 7).
 Physical examination, including ASA-PS score (Appendix 6).
 Vital signs (HR, RR, SpO2, SBP, DBP, MAP, Temp) after the subject has been resting supine
 Height, weight, and calculated BMI.
 12-lead ECG. ECG results collected within 30 days of Day 1 are acceptable for eligibility.
Clinical laboratory tests (hematology, blood chemistry, and urinalysis, etc.). Lab results
collected within 30 days of Day 1 are acceptable for eligibility. Required screening
laboratory samples must be collected and sent to the central laboratory within the screening
period.
 Conduct serum human chorionic gonadotropin (HCG) test (for women of childbearing
potential only).
 Instruct subject to fast at least 6 hours before surgery. Per investigator discretion, in
otherwise healthy patients, clear liquids may be ingested for up to 2 hours prior to surgery.
These liquids should not include alcohol. Examples of clear liquids include, but are not
limited to, water and fruit juices without pulp, carbonated beverages, carbohydrate-rich
nutritional drinks, clear tea and black coffee.
 Document AEs (after signing informed consent).

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Note: This study only collects the subjects' disease history within 6 months before screening and 
all previous medications used within 1 month before screening, and the above information 
should be recorded in the source medical record and the eCRF. 
7.1.2. 
Day of Surgery (Day 1) 
Enrolled subjects will be stratified by American Society of Anesthesiologists Physical Status 
(ASA-PS; I-II and III-IV), age (<65 and 65 years), and Body Mass Index (BMI <35 and 35 
kg/m2). 
The screening BMI will be used to calculate study drug dose. Eligible subjects will be 
randomized in a 2:1 ratio to receive either HSK3486 0.4/0.2 mg/kg (i.e., 0.4 mg/kg IV slow 
injection over 30 [±5] seconds followed by an additional 0.2 mg/kg IV top-up dose if needed) or 
propofol 2.0/1.0 mg/kg (i.e., 2.0 mg/kg IV slow injection over 10 [±2] seconds followed by an 
additional 1.0 mg/kg top-up dose if needed) in a blinded manner. 
All throughout the preinduction and induction periods a timing device must be used to allow 
accuracy and sequencing of necessary assessments.  
On the day of surgery, the following procedures as indicated in the Schedule of Assessments will 
occur: 
Prior to entering the operating room: 
 Refer to 7.1.1 to ensure required screening laboratory samples have been collected and sent
within the screening period to the central laboratory.
 Measure vital signs (HR, RR, SpO2, SBP, DBP, MAP, Temp) to confirm consistent with
Conduct urine human chorionic gonadotropin (HCG) test (for women of childbearing
potential only).
 Obtain 12-lead ECG at Day 1 visit only if the subject reports any cardiopulmonary
symptoms that warrant an ECG, in the opinion of the investigator. The 12-lead ECG should
be repeated to assess its compliance with the inclusion/exclusion criteria.
 Verify inclusion/exclusion criteria.
 Premedication allowed except sedative-hypnotics, analgesic drugs or any medications that
relieve pain.
 Orient subject to the use of the NRS pain assessment.
Preinduction period: 
 Confirm preoperative readiness (subject is hemodynamically stable and has followed

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preoperative instructions, and there is no evidence of acute illness such as fever). 
 Obtain vital signs (heart rate [HR], respiratory rate [RR], oxygen saturation [SpO2], systolic
blood pressure [SBP], diastolic blood pressure [DBP], mean arterial pressure [MAP]),
Temperature [Temp]; baseline value will be the measurement immediately prior to initiation
of study drug administration.
 Start maintenance IV infusion (NS, LR, or 5% dextrose solution).
Attach 3-lead or 5-lead electrocardiogram (ECG) to subject and monitor continuously. In the
event an abnormality is noted with 3-lead or 5-lead ECG, clinical significance of an
abnormality should be documented and the investigators will decide whether to add a 12-
lead ECG.
 Administer oxygen via face mask ( 4 L/min) at least 2 minutes before study drug
administration. Subsequently, the investigator can adjust the oxygen flow according to the
specific situation of the subject.
 Attach BIS monitor to subject (either in preoperative anesthesia unit or in operating room,
depending on local practice). Record the three most recent BIS values preceding midazolam
administration.
 Subjects will receive IV midazolam at dose 0.04 mg/kg, up to 3 mg maximum per subject
over 15 [±2] seconds at 5 minutes [±30 seconds] prior to initiation of induction agents as
premedication. The midazolam dose can be reduced according to patient’s age and
comorbidities as per the anesthetist’s discretion. The start and end time of midazolam
administration should be recorded. The end time of midazolam administration will begin the
window to start of IP.
 Subjects will receive preinduction IV fentanyl at a dose of 1 mcg/kg rounded up to the
nearest 25 mcg, up to 100 mcg maximum over 15 [±2] seconds at 2 minutes [±10 seconds]
prior to initiation of induction agents. The fentanyl dose can be reduced according to
patient’s age and comorbidities as per the anesthetist’s discretion. The start and end time of
fentanyl administration should be recorded. The end time of fentanyl administration will
begin the window to start of IP.
 Information about any adverse events (AEs) and concomitant medications will be recorded.
 Note: Throughout the preinduction and induction periods, a timing device must be used to
allow accuracy and sequencing of necessary assessments.
Induction period of general anesthesia: 
 Monitor vital signs (HR, RR, SpO2, SBP, DBP, MAP, Temp) continuously. HR, SBP, DBP,
MAP, Temp should be recorded once every 2 minutes [±30 seconds], SpO2 value should be
recorded once every 1 minute [±15 seconds] from the start of study drug administration (for
every time point) for 30 minutes post start of study drug administration for the judgment of

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key secondary endpoint. RR should be recorded once every 2 minutes [± 30 seconds] from 
the start of study drug administration until administration of rocuronium.   
Note: Vital signs (HR, RR, SBP, DBP, MAP, Temp) monitor should be set to cycle every 1 
minute to obtain vitals at least every 2 minutes (in case cycle requires longer than 1 minute). 
Administer IV study drug (HSK3486 0.4 mg/kg or propofol 2.0 mg/kg) into vein located on
the back of right or left hand (this IV location is strongly preferred rather than mandatory) at
a port closest to the IV cannula (IV injection time: 30 [±5] seconds).
The MOAA/S will be evaluated every 30 [±10] seconds after end of study drug
administration until MOAA/S 1 is reached. Timepoints for MOAA/S 
seconds should be recorded.
If MOAA/S is still >1 at 1 minute [±10] seconds post end of study drug administration, a
top-up dose of 50% of the initial calculated dose of study drug will be given to the subject
(IV injection time: 10 [±2] seconds). Start and end times of top-up dose administration will
be recorded. The MOAA/S will be evaluated every 30 [±10] seconds post end of the top-up
dose administration.
 If MOAA/S is still >1 at 2 minutes [±10 seconds] post end of the top-up dose
administration, then the rescue drug, propofol, will be given (in both treatment groups). The
rescue dose is prepared as the initial calculated dose (100%) and administered per propofol
guidelines. The MOAA/S will be evaluated every 30 [±10] seconds post end of rescue drug
 Administration of study drug must be initiated at 5 minutes [±30 seconds] post midazolam
preinduction medication administration stop time. Top-up doses should be administrated
within 10 seconds once MOAA/S is evaluated >1.
 Injection-site pain is evaluated verbally by Numeric Rating Scale (NRS; Appendix 3). Upon
administration of study drug injection, the investigator should immediately ask the subject to
rate his or her pain at injection-site. As a general guidance, the evaluation should be done
multiple times with the first evaluation occurring typically within 15 seconds after initiation
information such as the occurrence and severity of injection pain (NRS 0-10) shall be
recorded. The maximum (highest value) injection-site pain will be recorded in EDC as NRS.
Pain during the injection of study drug in the hand/arm should be distinguished from
unrelated puncture pain from the IV site or due to adhesive tape at the IV site.
 Post the end of initial study drug administration, the eyelash reflex will be evaluated every
30 [±10] seconds until loss of eyelash reflex. If there is a top-up dose, eyelash reflex will be
evaluated every 30 [±10] seconds post end of the top-up dose administration until loss of
eyelash reflex. If there is rescue drug given, eyelash reflex will be evaluated every 30 [±10]
seconds post end of the rescue drug until loss of eyelash reflex. The time of loss of eyelash
reflex should be recorded. Timepoints for loss of eyelash reflex within 1 to 40 seconds
should be recorded.

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BIS will be monitored continuously; record baseline BIS value prior to administering study
drug. BIS values will be collected at the following timepoints from start of initial study drug
administration: every 30 [±10] seconds until 5 minutes, then every 2 minutes [±30 seconds]
until 30 minutes and then every 30 [±2] minutes for duration of the surgery.
Monitor 3-lead or 5-lead electrocardiogram (ECG) continuously. Abnormalities of clinical
significance and non clinical significance should be recorded. The investigators decide
whether to add a 12-lead ECG based on the Subject's condition.
administered for neuromuscular blockade to perform endotracheal intubation. The start and
end times of rocuronium bromide administration should be recorded. RR collected until
initiation of administration of rocuronium.
Note: For subjects with BMI > 40 kg/m2, the rocuronium dose may be modified per
investigator discretion.
Note: 
2: If the exact calculated Rocuronium dose cannot be
drawn, round up or down to the nearest mg (whole number) depending on investigator
preference/SOC.
Note: Additional IV rocuronium is allowed per investigator’s discretion only in response to
clinical symptoms during tracheal intubation, such as gag reflex and movement et al, or
during surgical procedures. The start and end times of additional rocuronium bromide
administration should be recorded.
 Intubate subject once neuromuscular blockade has taken effect; if using twitch monitor,
intubate once no twitches are noted. The start and end times of first and subsequent
intubation attempts should be recorded.
 During endotracheal intubation, evaluate and record clinical symptoms and/or signs for
inadequate depth of anesthesia, such as lacrimation, movement, vomiting, coughing,
laryngospasm, bucking, swallowing reflex, and/or bronchospasm, etc. for at least 15 minutes
from start of study drug administration.
 Between start of study drug administration and prior to the administration of rocuronium
bromide, evaluate and record respiratory depression.
Note: Respiratory depression includes apnea, defined as absence of thoracic movement
lasting >30 seconds, prior to the administration of rocuronium bromide, or hypoxia, defined
as SpO2 <90% lasting >30 seconds, or life-threatening apnea or hypoxia requiring immediate
intervention.
 Evaluate and record cardiac depression from start of study drug administration until the
subject leaves the operating room.
Note: Cardiac depression is defined as SBP <90 mmHg lasting >2 minutes plus requiring
medical intervention such as inotropes, vasopressors, or IV fluid resuscitation, or life-
threatening hypotension requiring immediate intervention.

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 Sevoflurane (an inhalation anesthetic agent) will be used for maintenance of general
anesthesia. Initiate sevoflurane within 60 seconds after successful endotracheal intubation.
The end-tidal concentration of sevoflurane should be 1.5-2.0 % (The start and end time of
administration should be recorded).
 Information about any adverse events (AEs) and concomitant medications will be recorded.
Maintenance period of general anesthesia:
 During the maintenance period of general anesthesia, the inhalational anesthetic agent
sevoflurane will be used according to routine clinical practice, and the end-tidal
concentration of sevoflurane should be 1.5-2.0 % within 15 minutes after initiation of study
drug administration and can be adjusted to the desired effect after that.
 Propofol should not be used at any time all throughout the maintenance period.
 During the maintenance period, end-tidal sevoflurane concentration, will be monitored per
standard of care.
 The BIS will be monitored continuously per standard of care, and BIS values will be
collected at the following timepoints post start of initial study drug administration: every 30
[±10] seconds until 5 minutes, then every 2 minutes [± 30 seconds] until 30 minutes and
then every 30 [±2] minutes for the duration of the surgery.
 Additional IV fentanyl or other analgesic drug may be administered for intraoperative
analgesia only after initiation of sevoflurane, preferably after 15 minutes following the
initiation of study drug administration, start time and end time of initial study drug
administration should be recorded; if additional drug used, the start and end time of
administration should be recorded.
 Subject management during surgery needs to follow routine best practice which includes
Antiemetics Ondansetron, or other 5 HT-3 antagonists and/or Dexamethasone.
Monitor 3-lead or 5-lead ECG continuously. Abnormalities of clinical significance and non
clinical significance should be recorded. The investigators decide whether to add a 12-lead
ECG based on the Subject's condition. Information about any adverse events (AEs) and
concomitant medications will be recorded.
 Blood sample for population PK study will be collected at the time points as indicated in
(Appendix 5).
 Information about any adverse events (AEs) and concomitant medications will be recorded.
7.1.3. 
Follow-up Period 6 (±2) hour Post Study Drug and 24-hour Post Study Drug 
Phone Contact [Day 2] 
For the first 6 (±2) hour post study drug evaluation, study assessments and procedures will be 
assessed as indicated in Schedule of Assessments: 

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 Postoperatively, the subject will be monitored per facility/institution standard of care in the
PACU until the 6 (±2) hour follow-up assessments. However, when deemed discharge ready
per institution/facility policy, inpatients may be transferred to their rooms where the 6 hour
follow up assessments can be performed. For subjects undergoing outpatient surgery, upon
discharge readiness they may be released from the PACU but must remain in the
facility/institution as well as be accompanied by a responsible individual until the 6
(±2) hour follow-up assessments. The responsible party can be either a personal
acquaintance or personnel from the institution.
In the PACU, once the subject is alert and oriented, repeat NRS for recall of pain at time of
study drug administration and assess surgical awareness with recall using the Brice
Awareness Questionnaire (Appendix 4).
 After the surgery, vital signs (HR, RR, SpO2, SBP, DBP, MAP, Temp) will be assessed at 6
(±2) hours post study drug administration.
 Clinical laboratory tests (hematology, blood chemistry, and urinalysis) will be obtained at 6
(±2) hours post administration of study drug for shipment to central laboratory.
 Obtain 12-lead ECG at 6 (±2) hours post study drug administration, and as clinically
indicated.
Note: If surgery lasts >4 hours, vital signs, clinical laboratory tests, and ECG should be 
obtained 1 (+1) hour after completion of surgery. Duration of surgery is defined as time from 
study drug administration to time of transfer from operating room to recovery room or PACU. 
 Document AEs and concomitant medications.
 After the surgery, subjects may remain in the hospital or observation unit if required based
on standard of care and the clinical situation; however, if the subject is clinically stable after
the Day 1 follow-up assessments and appropriate to be discharged home per the judgement
of the investigator and surgeon, the subject may be released with supervision by a family
member or friend.
24-hour follow-up: Phone Contact
Subjects will be evaluated by a follow-up telephone call 24 [±6] hours after study drug
administration.
Information about any AEs and concomitant mediations will be collected and recorded.
7.1.4. 
Phone Contact (Day 8) 
Subjects will be evaluated by a follow-up telephone call 7 (±2) days after surgery: 
 Surgical awareness with recall will be re-assessed using the Brice Awareness Questionnaire.
 Information about any AEs and associated medications will be collected and recorded.

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7.2. Efficacy Assessments 
7.2.1.
Success Rate of Anesthesia Induction
Success rate of anesthesia induction: The proportion of subjects with successful anesthesia 
induction in all subjects of the group. 
A successful anesthetic induction should meet both of following conditions: 
1.
and
2.
One or less top-up dose required without using any rescue drugs.
Scoring criteria: According to the MOAA/S scale (Appendix 1), observation and evaluation 
are carried out stepwise, and the cross-level evaluation should not be carried out.  
MOAA/S scoring: The subject's name is called during induction to observe the subject's response 
to name calling with a normal tone. Lightly stimulate or shake the subject after the subject falls 
asleep and observe the subject's response to painful stimulation. If the subject does not respond 
to the light stimulus or shaking but responds to painful stimulation (by squeezing the trapezius 
muscle), the MOAA/S score is 1 and the time from the end of the dosing of the study drug to 
corded. 
7.2.2. 
Composite Endpoint to Evaluate Desired Depth of Anesthesia 
Composite endpoint of desired depth of anesthesia for general elective surgery is defined if all 
following criteria are met:  
Proportion of successfully induced subjects treated with HSK3486 compared to propofol
with no any clinical symptoms in response to tracheal intubation, such as lacrimation,
movement, vomiting, coughing, laryngospasm, bucking, swallowing reflex or
bronchospasm, or an event where no SBP, DBP, or MAP increases more than 20% from
baseline in response to any major operational procedures or noxious stimulus in defined
period ,within 15 minutes post initiation start of study drug administration or up to the start
of second tracheal intubation attempt.
 Subjects maintain desired depth of anesthesia for general elective surgery within 15 minutes
post initiation of study drug administration with BIS as an objective assessment (after
reaching the initial lowest value, BIS remains sustainable level at not more than 60 during
15 minutes after study drug administration).
Note: Target BIS 40 to 60 for general anesthesia (see Appendix 2 for BIS guidance).
The BIS sustainable level at not more than 60 is defined as not more than 1 episode with BIS
value observed >60 after reaching initial lowest value, in defined period.

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 No significant respiratory depression, such as apnea, prior to the administration of
rocuronium bromide.
Note: For the composite endpoint, respiratory depression includes apnea, defined as absence
of thoracic movement lasting >30 seconds, or hypoxia, defined as SpO2 <90% lasting >30
seconds, or life-threatening apnea or hypoxia requiring immediate intervention.
No significant cardiac depression indicated by blood pressure decrease that requires
intervention, i.e., vasopressors and/or IV fluid resuscitation.
Note: For the composite endpoint, cardiac depression is defined as SBP <90 mmHg lasting
>2 minutes plus requiring medical intervention such as inotropes, vasopressors, or IV fluid
resuscitation, or life-threatening hypotension requiring immediate intervention.
7.2.3. 
Injection-site Pain 
Injection-site pain is evaluated verbally during study drug administration using the NRS, ranging 
from 0 (no pain) to 10 (worse imaginable pain) (see Appendix 3). Recollection of pain during 
study drug administration will be assessed in the PACU once the subject is awake and oriented. 
During the baseline visit, the subject should be oriented to the NRS for the evaluation of 
injection-site pain. Upon initiation of study drug administration, the investigator should 
immediately ask the subject to rate his or her pain. As a general guidance, the evaluation should 
be done multiple times with the first evaluation occurring typically within 15 seconds after 
initiation of study drug administration
Related information such as the occurrence and severity of injection pain (NRS 0-10) shall be 
recorded. The maximum (highest value) injection-site pain should be recorded. Pain during the 
injection of study drug in the hand/arm should be distinguished from unrelated puncture 
pain from the IV site or due to adhesive tape at the IV site. When injection pain occurs, the 
subject’s pain should be graded verbally using the NRS (0-10) and should be recorded. The 
subject should be asked to rate the pain on a scale from 0, which is no pain, to 10, which is the 
worst pain imaginable. Ask the subject to point to the number on the scale that best represents 
the intensity of the pain now. In the PACU, once the subject is alert and oriented, repeat NRS for 
recall of pain at time of study drug administration.
7.2.4. 
 Time to Successful Anesthesia Induction 
The time to successful anesthesia induction is evaluated during the induction period and is 
defined as the time from the end of the first administration of the study drug to the time when 

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7.2.5. 
 Time to Loss of Eyelash Reflex 
The time to loss of eyelash reflex is evaluated during the induction period and defined as the 
time from the end of the first administration of the study drug to the time when eyelash reflex is 
lost. 
7.2.6. 
Successful induction without non-optimal anesthetic effects 
The proportion of subjects with successful induction without non-optimal anesthetic effects 
within 15 minutes post initiation of administration of study drugs will be analyzed using the 
similar statistical methods as for the secondary composite efficacy endpoint. 
7.2.7. 
Changes in the Bispectral Index 
From the start of study drug administration to the end of the surgery, the subjects are 
continuously monitored for their BIS, which is recorded at specified time points to understand 
the changes in the BIS of the subjects throughout the entire anesthesia period (see Appendix 2) 
for BIS guidance). 
7.2.8. 
Brice Awareness Questionnaire 
Recall of awareness during surgery, assessed postoperatively, will be evaluated using the Brice 
Awareness Questionnaire (see Appendix 4).
7.2.9. 
Use of Study Drugs and Alternative Drugs 
The use of HSK3486 (including the time and the amount of top-up dose) is evaluated from the 
end of study drug administration to the time of successful induction. The use of propofol 
(including the time and the amount of top-up dose) is evaluated from the end of study drug 
administration to the time of successful induction. The proportion of subjects using the 
alternative drug (propofol) will also be calculated for each dose group. 
7.3. Safety and Tolerability Assessments 
7.3.1. 
Adverse Events 
Adverse event definitions and assignment of severity and causality are detailed in (Appendix 9). 
Adverse events will be elicited from the subject (or, when appropriate, from a caregiver, 
surrogate, or the subject’s legally authorized representative) by the study site staff using a 
non-leading question such as “How are you feeling today?” or “Have you had any health 
concerns since your last visit?”  

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The investigator and any designees are responsible for detecting, documenting, and reporting 
events that meet the definition of an AE or SAE and remain responsible for following up AEs 
that are serious, considered related to the study treatment or the study, or that caused the subject 
to discontinue the HSK3486 (see Section 4.3.2). 
When possible, AEs should be reported as the unifying diagnosis rather than individual signs and 
symptoms of an underlying condition (e.g., fever, cough, and elevated white blood cells [WBC] 
associated with pneumonia should be reported as pneumonia). Laboratory values themselves are 
usually not AEs, especially when a component of the underlying AE (e.g., an elevated WBC 
associated with pneumonia should not be reported as a separate AE). 
If arterial blood pressure is <90 mmHg and greater than a 30% decrease from baseline, an 
adverse event will be recorded. 
AEs Do Not Include the Following:
 Anesthetic effect-related sleepiness, somnolence, or even loss of consciousness occurring
during administration (i.e., the intended therapeutic effect of the drug under study)
 Chronic diseases that are present before participating in the clinical study
 Elective medical examination or surgery scheduled prior to enrollment into the study
 Overdose of the study drug or concomitant medication not resulting in any signs or
symptoms
 Lack of efficacy should be captured as a treatment failure for clinical efficacy, not as an AE.
7.3.2. 
Reporting Serious Adverse Events 
For the definition of an SAE please see Appendix 9. All SAEs, occurring after the signing of the 
ICF until the end of study and regardless of study drug relationship, must be reported by SAE 
Electronic Case Report Form (eCRF) form within the clinical database (Medidata RAVE) within 
24 hours of obtaining knowledge of the event. The Investigator will be responsible for reporting 
serious adverse events to the IRB/EC.
In the case of clinical database outage or the site is unable to access the system, the investigators 
will be requested to complete a Safety Event Report Form in English and report it by facsimile or 
email to Rho. The facsimile report should include all available information requested on the SAE 
Form. Initial reports of SAEs should never be left on telephone voicemails. Always email or fax 
SAE reports and follow with a telephone call if needed. 
Serious Adverse Event Reporting Contact Information:

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The SAE Form will collect data surrounding the event (e.g., the nature of the symptom[s], time 
of onset in relation to initiation of therapy, duration, intensity, and whether or not therapy was 
interrupted or discontinued). The investigator’s assessment of the probable cause of the event 
will also be included. In addition, relevant medical history, concomitant medications, laboratory 
and diagnostic tests reports, and procedures as well as all pertinent medical information related 
to the event will also be collected. 
Rho will forward SAE queries requesting incomplete or missing information directly to the 
investigator. It is the investigator’s responsibility to be diligent in providing this information 
back to Rho as soon as it is available.   
If an Investigator becomes aware of a serious adverse event within 30 days after the last dose of 
study drug and it is considered by him/her to be caused by the study drug with a reasonable 
possibility, the event must be documented and reported through completion of the adverse event 
eCRF. 
7.3.3. 
Adverse Events of Special Interest 
The AESIs due to pharmacological effect of an anesthetic agent include hypoxemia, 
bradycardia, severe hypotension, allergy/anaphylaxis, cardiac arrythmia and QTc prolongation 
which are defined as follows: 
Hypoxemia due to respiratory depression is defined as SpO2 <90% with duration of >30
seconds. Hypoxemia is evaluated from the initial dose of study drug until the subject leaves
the operating room. Those occurring within 15 minutes from the end of the last dose of
study drug will be categorized as drug related unless an obvious cause is ascertained.
Bradycardia is defined as a heart rate of <45 beats/minute that lasts >30 seconds and
requires intervention (treatment). Bradycardia is evaluated from the initial dose of study

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drug until the subject leaves the operating room. Those occurring within 15 minutes from 
the end of the last dose of study drug will be categorized as drug related unless an obvious 
cause is ascertained.
Severe hypotension is defined as an SBP <90 mmHg that lasts >2 minutes requiring
treatment. Severe hypotension is evaluated from the initial dose of study drug until the
subject leaves the operating room. Those occurring within 15 minutes from the end of the
last dose of study drug will be categorized as drug related unless an obvious cause is
ascertained.
Allergy/anaphylaxis may include angioedema, bronchospasm, erythema, and hypotension.
Allergy/anaphylaxis is evaluated from the initial dose of study drug until Day 2 phone
contact.
Cardiac arrhythmia is any rhythm not arising from the sinus node (atrial fibrillation, atrial
flutter, Torsade de pointes). Cardiac arrythmia is evaluated from the initial dose of study
drug until 6 (±2) hours post study drug.
QTc prolongation: QTc intervals of > 450 ms in males and > 470 ms in females occurring
within 15 minutes from the end of the last dose of study drug are considered QTc
prolongation. Patients who reach an absolute QT interval of more than 500 ms upon QTc
correction for rate should be reassessed, and immediate action should be taken to correct
any possible concomitant risk factors [15, 16].
Adverse events of thrombogenicity/hemolytic disorders will be considered an AESI.
AESI start time, end time (both should be accurate to the second and the duration should be
calculated) and treatment measures should be recorded (if any).
Note: Vital sign values out of range (or suspected as being erroneous) should be confirmed 
with a second measurement before recording as the confirmed value. 
Note: Since injection-site pain has been recorded and analyzed in the efficacy endpoints, it 
is no longer recorded in the Safety endpoints (see section 7.2.3) for injection-site pain 
evaluation. 
 
Both HSK3486 and propofol cross the blood-brain barrier and have CNS effects, which are the 
intended pharmacological action of the drug (e.g., sedation, anesthesia/unconsciousness). Any 
untoward change from baseline that is not the intended or desired effect of the study drug should 
be reported as an AE. Investigators and site personnel should monitor for and report abuse-
related AEs of interest, which include: euphoria, dissociative effects, hallucinations, psychosis, 
changes in mood, impaired cognition, attention and psychomotor effects, and inappropriate 
affect; overdoses, abuse, and misuse.   

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For all abuse-related AEs of interest, the time of onset and duration of the event, dose 
administered, severity and outcome should be recorded. 
While not AEs, any subject dropouts and lost or unaccounted for drug product (which could be 
indicative of abuse-related aberrant behavior) needs to be documented and recorded by site 
personnel. 
7.3.4. 
Contraception and Pregnancy
Currently, the effects of HSK3486 on the fetus are still unknown. Therefore, female subjects of 
childbearing potential and male subjects with female partners of childbearing potential are 
required to adopt appropriate contraceptive measures before (from screening) and during the 
study (30 days post study drug administration). For male subjects who have not been sterilized, 
their female partners of childbearing potential must also use appropriate contraception. Effective 
contraceptive measures include female bilateral tubal ligation, male vasectomy, hormone 
contraceptives (oral, implanted, injected and transdermal), the combination of intrauterine 
contraceptive device and barrier contraceptive (condoms, cervical caps) or abstinence. Male 
subjects should refrain from donating sperm from Day 1 to 30 days post study drug 
administration.
If a female subject or a female sexual partner of a male subject who has taken the study drug 
becomes pregnant during the study period (from signing ICF to 30 days post study drug 
administration), the investigator should immediately record the pregnancy information and fill 
out a pregnancy-specific report and notify the Contract Research Organization (CRO) designated 
by the Sponsor within 24 hours of being informed of the event. The investigator should also 
report the event to the IRB/EC in accordance with the ethical requirements of each study site.
The pharmacovigilance personnel of the CRO should review the pregnancy report form in 
accordance with the standard operating procedures and report it to the Sponsor within one 
business day of being informed of the event. If the event occurs during the study period, the 
pregnant female subject should withdraw from the study for reasons of protocol deviation. 
A pregnancy event should be followed up to the end of pregnancy or 8 weeks after the fetus is 
born. The follow-up information of pregnancy events is recorded in the source file. 
If pregnancy results meet SAE criteria, i.e., spontaneous abortion, induced abortion, stillbirth, 
neonatal death, and congenital malformations (including deformities in aborted babies), the 
investigator shall report in accordance with the SAE reporting procedure.  
7.3.5. 
Clinical Laboratory Evaluations 
Laboratory tests include the following: 

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 Blood routine (Hematology) (about 2 mL of blood sample): WBC count with differential (%
and absolute count) including neutrophils, lymphocytes, monocytes, basophils, eosinophils;
red blood cell (RBC) count, Hb, hematocrit (HCT), MCH, MCHC, MCV and platelet count
(PLT).
 Blood biochemistry (about 5 mL of blood sample): liver function (aspartate aminotransferase
[AST], alanine aminotransferase [ALT], alkaline phosphatase [ALP], gamma-glutamyl
transferase [GGT], total bilirubin [TBIL], total protein [TP], albumin [ALB], lactate
dehydrogenase [LDH]), blood lipids (triglycerides [TG], cholesterol [TC], low-density
lipoprotein [LDL], high-density lipoprotein [HDL]), electrolytes (sodium [Na], potassium
[K]), chloride [Cl], calcium [Ca], phosphorus [P], bicarbonate [HCO3], renal function (uric
acid [UA], blood urea nitrogen [BUN], creatinine [Cr]), blood glucose (Glu), and thyroid
stimulating hormone (TSH).
 Urinalysis (about 7 mL of fresh urine sample): color and appearance, urinary protein (PRO),
urinary glucose (GLU), microscopic exam including urinary WBCs and RBCs, urinary
ketone bodies (KET), pH and specific gravity, leukocytes, bilirubin, nitrite, and occult blood.
Laboratory tests are performed in the screening period and 6 (±2) hours post administration of 
study drug except for TSH. TSH is only drawn at the screening visit for subjects with known 
hypothyroidism and/or on thyroid-hormone replacement treatment (i.e., thyroxine), or subjects 
suspected to have thyroid dysfunction based on clinical laboratory and physical exam. In those 
cases where local laboratory results are utilized to confirm eligibility, required screening 
laboratory samples must be drawn and sent to the central laboratory within the screening period. 
Local laboratory results will be maintained in the source documents but not be entered into the 
eCRF.    
For cases where central and local labs are drawn within the screening period to confirm 
eligibility, blood volumes noted above in bullets 1 and 2 will be increased up to approximately 
15 mL total. Urine volume noted in bullet 3 will be increased up to approximately 15 mL.  
7.3.6. 
Vital Signs, Physical Examination, and Other Safety Evaluations 
 
Vital signs include RR, HR, SpO2, Temp, BP (SBP, DBP, and MAP).   
During screening, vital sign examination should be performed after resting for at least 5 minutes. 
If the results exceed the inclusion/exclusion criteria and are confirmed after repeated tests, the 
subject shall not be enrolled. 

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For the pre-anesthetic preparation period, after the anesthetic monitor is mounted, baseline vital 
signs values will be the measurement immediately prior to initiation of study drug 
administration.  
During anesthesia induction period, RR should be recorded once every 2 minutes [±30 seconds] 
from the start of study drug administration until administration of rocuronium. During anesthesia 
induction and maintenance period, vital signs are continuously monitored, and values are 
recorded once every 2 minutes [±30 seconds] (including vital signs monitored at the start of 
medication) for 30 minutes post study drug administration (SpO2 value should be recorded once 
every 1 minute [±15 seconds]), and then at 6 [±2] hours post study drug administration.  
If arterial blood pressure is <90 mmHg and greater than a 30% decrease from baseline, an 
adverse event will be recorded.
 
Physical examinations include skin, head, and neck, cardiovascular system, respiratory system, 
gastrointestinal system, psychological and neurological system, and musculoskeletal system. 
ASA-PS and Mallampati (oropharyngeal exam) classifications will also be evaluated. These 
assessments will be completed at screening, and a focused physical exam will be repeated as 
needed to assess AEs.
 
In the 12-lead ECG examination, the values of QT interval, QTcF interval, PR interval, QRS 
interval, RR interval and their clinical significance determinations are recorded. The 12-lead 
ECG examination is done during the screening period. 12-lead ECG results collected within 30 
days prior to Day 1 may be used to confirm eligibility. Obtain 12-lead ECG within the screening 
period only if the subject reports any cardiopulmonary symptoms that warrant an ECG, in the 
opinion of the investigator. A 12-lead ECG will be obtained at 6 [±2] hours post study drug 
administration and as clinically indicated. During the surgery, a 3-lead or 5-lead ECG (i.e., 
rhythm strip) is monitored continuously. If the 3-lead ECG shows clinically significant 
abnormalities, the investigators will decide whether to add a 12-lead ECG based per 
investigator’s discretion. 
7.3.7. 
Use of Additional Medication or Any Interventions
Administration of additional medication or any interventions including medical interventions, 
e.g., administration of vasoactive drugs, necessary due to a clinically relevant change in BP,
should be documented.

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7.4. Pharmacokinetic Analysis 
To maintain the blind, all subjects should undergo PK blood sampling regardless of their groups. 
However, only the blood samples in the HSK3486 group are tested and analyzed; unless 
requested by the Sponsor or its designated personnel, the propofol group may also be analyzed. 
To ensure the accuracy of the PK data, it is necessary to accurately record the actual start and 
end time of dosing, the dose, and the blood sampling time of each subject. 
The blood collection time points are listed in Appendix 5. Approximately 2 mL of venous blood 
is collected at each time point.
For the requirements of collection, processing, transportation, and analysis of blood samples, 
please see “Central Laboratory Manual”. 

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8. SAMPLE SIZE AND DATA ANALYSES
8.1. Determination of Sample Size 
A total of 399 subjects (266 in HSK3486 0.4/0.2 mg/kg group and 133 in propofol 2.0/1.0 mg/kg 
group) need to be enrolled 2:1 into this study based on the following assumptions: 
 For the primary endpoint, a sample size of 215 subjects will give at least 90% power
assuming that type I error is 0.025 (1-sided), the success rate of general anesthesia induction
of HSK3486 and propofol are both 97%, and non-inferiority margin (NIM) is -8%.
For the key secondary endpoint of incidence of injection-site pain, a sample size of 365
subjects will give 90% power (providing the first primary endpoint is statistically
significant) assuming 
0.015 (2-sided) and the proportion of subjects who meet the
endpoint criteria of any injection-site pain are 6.8% and 20.5% for HSK3486 and propofol,
respectively.
 For the key secondary composite endpoint, a sample size of 338 subjects will give 90%
power (providing the first primary endpoint is statistically significant) to the superior testing,
assuming 
35 (2-sided), the proportion of subjects with successful induction,
maintained desired depth of anesthesia for general elective surgery, without significant
cardiac and respiratory depression within the 15 minute post initiation start of study drug
administration observation period and no significant respiratory depression (prior to
administration of rocuronium bromide) of HSK3486 and propofol are 82% and 65%,
respectively (i.e., 17% treatment effect).
To power statistical testing for all three endpoints at 90% and provide sufficient safety data, 399 
subjects will be randomized and treated in this study (266 in HSK3486 0.4/0.2 mg/kg group and 
133 in propofol 2.0/1.0 mg/kg group).  
Enrolled subjects will be stratified between the 2 treatment groups by ASA-PS grade I to II and 
2. The stratification schema will be 
described in the Statistical Analysis Plan. 
8.2. Analysis Populations 
Full Analysis Set (FAS): All randomized subjects who have received any dose of the study drug 
(HSK3486 or propofol).  
Per Protocol Set (PPS): All subjects from FAS who have completed primary efficacy endpoint 
measurement will be considered for PPS. Subjects with any important protocol deviations will be 
reviewed after database lock and prior to study unblinding and may be excluded from PPS if 
these important deviations will impact the efficacy evaluations.  

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Safety Set (SS): Includes all randomized subjects who have received any dose of the study drug 
and have post-dose safety assessment data.
The FAS will be the primary analysis population for efficacy and PPS will be the supportive 
analysis population for efficacy analysis. Safety endpoints will be analyzed using the SS. 
8.3. General Considerations 
Continuous variables will be summarized by the standard descriptive statistics: number of 
subjects (n), mean, standard deviation (SD), median, minimum, and maximum. Frequency of 
subjects or events and percentages will be summarized in categorical variables. Details of the 
statistical analyses will be given in a separated Statistical Analysis Plan (SAP).
8.4. Efficacy Analysis 
8.4.1. 
Primary Efficacy Outcome Measure
The statistical null hypothesis to be tested is: 
: 
 
With the alternative hypothesis: 
: 
>
 
Where pt and pc are the anesthesia success rates for the HSK3486 and propofol arms, 
respectively, and is the NIM. This hypothesis will be tested at the 1-
 
Success rate of general anesthesia induction in both groups and rate difference between groups 
and its 95% confidence interval (CI) are estimated by Farrington-Manning method in the FAS. 
The stratification factors will be included in the analysis. The lower limit of 95% CI of rate 
difference will be compared with the NIM of -8% to confirm the establishment of non-
inferiority. The FAS subjects who are non-evaluable for anesthesia success will be counted as 
treatment failures. 
8.4.2. 
Justification of the Non-inferiority Margin 
The NIM for this study is based on the expectation that the active control, propofol, and 
with no more 
than 1 top-
for loss of assay sensitivity (i.e., loss of efficacy) from historical performance or loss of the 
“constancy assumption.” Furthermore, the exercise of attempting to identify the M1 based on 

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historical literature would lead to an unacceptably large margin, then applying clinical judgement 
to establish the M2 (often 50% of the M1) (Food and Drug Administration [FDA] Guidance for 
Industry: Non-Inferiority Clinical Trials to Establish Effectiveness, 2016) would lead to a NIM 
>10%, which permits too much uncertainty to be acceptable or useful. Using a NIM of 8% is
clinically relevant and meaningful for this indication.
8.4.3. 
Secondary Efficacy Outcome Measures
The proportion of subjects with successful induction, who maintain desired depth of anesthesia 
for general elective surgery, and without significant cardiac and respiratory depression between 
the time of successful induction and 15 minutes post initiation of study drug administration (prior 
to administration of rocuronium bromide) in both groups and difference between groups and 
95% CI will be calculated. The p-value for comparison between groups will be obtained based 
on the Cochran-Mantel-Haenszel Chi-squared test using the stratification factors in the study 
randomization. 
The proportion of the subjects with any injection-
 
The median and its 95% CI of time to successful induction of general anesthesia and time to the 
disappearance of eyelash reflex will be provided by groups using the Kaplan-Meier (KM) 
method. The KM estimates and 95% Cis at selected time points will also be reported.  
The proportion of subjects with successful induction without non-optimal anesthetic effects 
within 15 minutes post initiation of administration of study drugs will be analyzed using the 
similar statistical methods as for the secondary composite efficacy endpoint. 
The change of BIS during the period of anesthesia post study drug administration up to 15 
minutes will be summarized descriptively by treatment group. 
The Brice Awareness Questionnaire results will be summarized descriptively by treatment group. 
The use of study drugs and remediation drugs will be summarized descriptively by treatment 
group and between-group p-values. 
8.4.4. 
Methods for the Control of Type I Error 
The primary endpoint of non-inferiority to propofol for successful induction of general  
anesthesia will be tested first. If the null hypothesis fails to be rejected, the testing stops. If the 
primary endpoint is statistically significant, then the key secondary endpoints will be tested in 
parallel using a weighted Bonferroni-Holm step-down approach with weights w1=0.7 for the 
proportion of subjects who maintain the desired depth of anesthesia, and w2=0.3 for the incidence 
of injection-site pain. In this procedure, the p-values for the secondary endpoints are divided by 

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their respective alpha levels, then the endpoint for which this calculation is smaller (i.e., test pj < 
j for j=arg min (p1/0.035, p2/0.015)) is tested. If the first test is rejected, then the other endpoint 
all other 
secondary endpoints will be descriptive, and any testing that may be done will not be adjusted to 
maintain Type I error.
8.5. Safety Analysis 
Safety endpoints include Aes, clinical laboratory test results, vital signs (supine) HR, SBP and 
DBP, RR, and SpO2, ECG findings, physical examination findings, and administration of 
additional medication or any interventions including medical interventions, e.g., administration 
of vasoactive drugs, necessary due to a clinically relevant change in BP. No formal statistical 
analysis of the safety data will be performed.
All Aes will be coded according to the MedDRA version 25.0 or later and graded for severity 
according to CTCAE version 5.0. The number and percentage of subjects with treatment-
emergent adverse events (TEAEs), SAEs, AESIs (Section 7.3.3), TEAEs related to study drug, 
SAEs related to study drug, TEAEs of Grade 2 or higher, TEAEs leading to treatment 
discontinuation, TEAEs leading to study discontinuation, and TEAEs leading to death will be 
summarized by system organ class (SOC), preferred term (PT), and groups. In addition, the 
severity of TEAEs and relationship to study drug will be summarized by SOC, PT, and treatment 
groups.  
Laboratory test variables will be summarized by treatment group and visit using descriptive 
statistics. Shift tables between baseline and post-baseline time points will be presented by 
laboratory test and treatment group. Laboratory tests with categorical results that cannot be 
analyzed by change from baseline or shift table analysis will not be included in these summaries 
but will be listed. 
Descriptive statistics of vital signs, pulse oximetry measurements, site pain on study drug 
administration with intensity rating on a verbal score, and ECG results at each visit will be 
presented.
Note: Since injection-site pain has been recorded and analyzed in the efficacy endpoints, it is no 
longer recorded in the Safety endpoints. 
An independent DMC will be involved in the review of all safety data at approximately 30% 
enrollment, and on an ad hoc (as needed) basis as described in Section 3.5, as well as described 
in the DMC Charter.   

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8.6. Pharmacokinetic Analysis 
The plasma concentration data from this study will be pooled with those from other clinical trials 
of HSK3486 to establish a population PK model. This model will be used to evaluate the effects 
of internal and external covariates on the PK of HSK3486.  
8.7. Interim Analysis 
No formal interim analyses will be conducted. 

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9. REFERENCES
1. Picard P, Tramer MR. Prevention of pain on injection with propofol: a quantitative
systematic review. Anesth Analg. 2000;90(4):963-9.
2. Vanlersberghe CF. Camu. Propofol Handb Exp Pharmacol, 2008;(182):227-52.
3. Gao JD, Zhao YJ, Xu CS, et al. Evaluation of entropy for monitoring the depth of
anesthesia compared with bispectral index: a multicenter clinical trial. Chin Med J.
2012;125(8):1389-92.
4. Gélinas C, Arbour C. Behavioral and physiologic indicators during a nociceptive
procedure in conscious and unconscious mechanically ventilated adults: similar or
different? J Crit Care. 2009;24:628.e7-17.
5. Joo HS, Perks WJ. Sevoflurane versus propofol for anesthetic induction: a meta-analysis.
Anesth Analg. 2000;91(1):213-9.
6. Sebel PS, Lowdon JD. Propofol: a new intravenous anesthetic. Anesthesiology.
1989;71(2):260-77.
7. Chan VW, Chung FF. Propofol infusion for induction and maintenance of anesthesia in
elderly patients: recovery and hemodynamic profiles. Journal of Clinical Anesthesia.
1997;8(4):317-23.
8. Dundee JW, Robinson FP, Mccollum JSC, et al. Sensitivity to propofol in the elderly.
Anaesthesia, 1986;41(5):482-5.
9. Doenicke AW, Roizen MF, Rau J, et al. Reducing pain during propofol injection: the role
of the solvent. Anesth Analg. 1996;82(3):472-47.
10. HSK3486 Injectable Emulsion Investigator’s Brochure, V9.0, Oct 10, 2023.
11. Jerry, et al. Lean body weight scalar for the anesthetic induction dose of propofol in
morbidly obese subjects. Anesth Analg. 2011 Jul;113(1):57-62.
12. Nightingal, et al. Peri-operative management of the obese surgical patient 2015:
Association of Anesthetists of Great Britain and Ireland Society for Obesity and Bariatric
Anesthesia. Anesthesia. 2015 Jul;70(7):859-76.
13. Dong D, et al. Morbid Obesity Alters Both Pharmacokinetics and Pharmacodynamics of
Propofol: Dosing Recommendation for Anesthesia Induction. Drug Metab Dispos. 2016
Oct;44(10):1579-83.
14. Ingrande, et al.  Dose adjustment of anesthetics in the morbidly obese. 2010 Dec;105
Suppl 1:i16-23.
15. Kishida H, Cole JS, Surawicz B. Negative U wave: a highly specific but poorly
understood sign of heart disease. Am J Cardiol. 1982 Jun;49(8):2030-6. Doi:
10.1016/0002-9149(82)90225-9. PMID: 6211085.

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16. Document E 14: Clinical Evaluation of QT/QTc Interval Prolongation and Proarrhythmic
Potential of Non-Antiarrhythmic Drugs, Rockville, Md: US Department of Health and
Human Services, Food and Drug Administration; October 2005.

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10. APPENDICES
Appendix 1 Modified Observer’s Assessment of Awareness/Sedation (MOAA/S) Scale 
The assessment for MOAA/S is performed stepwise and skipping of any grade is not allowed. 
The judgment of recovery can be based on a reverse order of scoring. 
Grade
Description
Grade 5 
Completely awake; responds readily to name spoken in normal 
tone 
Grade 4
Lethargic response to name spoken in normal tone 
Grade 3
Responds only after name is called loudly and repeatedly
Grade 2
Responds only after mild prodding or shaking
Grade 1 
Responds only after painful trapezius squeeze (including 
active and reflex withdrawals)
Grade 0
No response after painful stimulus (trapezius squeeze)

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Appendix 2  Bispectral Index (BIS)
The BIS Index is a number between 0 and 100 scaled to correlate with important clinical 
endpoints and electroencephalogram (EEG) states during administration of anesthetic agents 
(Figure 2). The BIS values near 100 represent an “awake” clinical state while 0 denotes the 
maximal electroencephalogram (EEG) effect possible (i.e., an isoelectric EEG).
Figure 2 
The BIS Index is scaled to correlate with important clinical endpoints during 
administration of anesthetic agent. 
It should be noted that the BIS Index range represents a continuum that correlates to the clinical 
state and expected responses (Figure 3).
This chart below reflects a general association between clinical state and BIS values. Ranges are 
based on results from a multicenter study of the BIS involving the administration of specific 
anesthetic agents. The BIS values and ranges assume that the EEG is free of artifacts that can 
affect its performance. Titration of anesthetics to BIS ranges should be dependent upon the 
individual goals established for each subject. These goals and associated BIS ranges may vary 
over time and in the context of subject status and treatment plan.

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Figure 3 
BIS Index Range: A Continuum of Clinical State and EEG Changes
Appendix 3  Numerical Rating Scale (NRS) for Grading of Injection-site Pain

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Appendix 4 Brice Awareness Questionnaire 
1) What is the last thing you remember before going to sleep?
-Being in the pre-op area
-Seeing the operating room
-Being with family
-Hearing voices
-Feeling mask on face
-Smell of gas
-Burning or stinging in the IV line
-Other [Free Text]:
2) What is the first thing you remember after waking up?
-Hearing voices
-Feeling breathing tube
-Feeling mask on face
-Feeling pain
-Seeing the operating room
-Being in the recovery room
-Being with family
-Being in ICU
-Nothing
-Other [Free Text]:
3) Do you remember anything between going to sleep and waking up?
-No
-Yes:
-Hearing voices
-Hearing events of the surgery
-Unable to move or breathe
-Anxiety/stress
-Feeling pain
-Sensation of breathing tube
-Feeling surgery without pain
-Other [Free Text]:
4) Did you dream during your procedure?
-No
-Yes:
-What about [Free Text]:
5) Were your dreams disturbing to you?
-No
-Yes

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6) What was the worst thing about your operation?
-Anxiety
-Pain
-Recovery process
-Functional limitations
-Awareness
-Other [Free Text]:

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Appendix 5 Pharmacokinetic (PK) Blood Samples Collection Timetable 
Phase
Time
Allowed Time 
Window 
PK Blood Sample 
Collection2 
Before dosing study drug 
Before the 1st dose 
of study drug 
administration
Within 10 minutes 
prior to study drug 
administration
X 
After the end of last dose of study drug1
2 minutes3
±30 seconds
X 
3 ~ 30 minutes 
Not applicable 
X 
60 ~ 120 minutes 
Not applicable 
X 
Note: 
1.
Up to 1 top-up dose of study drug is allowed. “The end of last dose of study drug” refers to
the last dose of study drug for induction of general anesthesia. This is calculated from post
study drug administration. For example, if the subject only receives the initial dose of study
drug and has achieved successful anesthesia induction, then “the end of last dose of study drug”
means the initial dose of study drug. If the subject receives an additional top-up dose in the
anesthesia induction, “the end of last dose of study drug” means the last top-up dose of study
drug.
2. All subjects are required to provide venous PK blood samples. The blood volume is
approximately 2 mL per sample. The PK blood samples will be taken from a second IV.
3.
For subjects requiring rescue medication, the 2-minute PK sample must be taken prior to
rescue medication being administered.

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Appendix 6 American Society of Anesthesiologists (ASA) Physical Status Grade 
Before anesthesia, the ASA classifies subjects into 5 grades according to their physical 
conditions and the risk of surgery:  
 ASA I: Normal and healthy. No systemic diseases other than local lesions.
 ASA II: A subject with mild systemic disease.
ASA III: A subject with severe systemic disease that results in functional limitations but not
incapacity.
ASA IV: A subject with severe systemic disease that is a constant threat to life and results in
incapacity.
ASA V: A moribund subject who is not expected to survive without the operation. If it is an
emergency surgery, mark “Emergency” or “E” before the evaluated level shown above.
Subjects assessed as ASA I or ASA II have good tolerability to anesthesia and surgery, and the 
anesthesia. The pre-anesthetic preparation should be adequate. Effective measures should be 
taken to prevent complications that may occur during anesthesia. Subjects assessed as ASA IV 
are under particularly high risks of anesthesia. Subjects assessed as ASA V are under extremely 
high risks of anesthesia and surgery for their extremely critical conditions, extremely poor 
tolerability to anesthesia, and death that may occur at any time. Thus, pre-anesthetic preparation 
is more Important and shall be adequate, careful, and comprehensive.

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Appendix 7 Mallampati Classification 
The Modified Mallampati classification is a simple scoring system to help predict difficult 
intubation. It relates the amount of mouth opening to the size of the tongue and provides an 
estimate of space available for oral intubation by direct laryngoscopy. Class I is present when the 
soft palate, uvula, and pillars are visible; Class II when the soft palate and uvula are visible; 
Class III when only the soft palate and base of the uvula are visible; and Class IV when only the 
hard palate is visible. Mallampati Class I and II predict easy laryngoscopy, Class III predicts 
difficulty, and Class IV predicts extreme difficulty.

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Appendix 8 
Drugs that May Prolong the QT Interval 
*use with caution only if medically necessary
Drugs that prolong QT interval (including but not limited to 
the following)
Antibiotics
Clarithromycin
Azithromycin
Erythromycin 
Roxithromycin 
Metronidazole 
Moxifloxacin
Antiarrhythmics
Quinidine
Sotalol 
Amiodarone 
Disopyramide 
Procainamide
Antipsychotics 
Fluphenazine 
Droperidol 
Haloperidol 
Thioridazine 
Pimozide
Clozapine 
Olanzapine
Chlorpromazine 
Quetiapine 
Antifungals 
Fluconazole 
Ketoconazole 
Itraconazole
Antimalarial drugs
Mefloquine
Chloroquine 
Antidepressants
Amitriptyline
Imipramine
Clomipramine 
Dosulepin 
Doxepin 
Floxitine 
Paroxetine 

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Sertralin 
Citalopram
Fluvoxamine 
Anti-tumor drugs
Anthracyclines
5-fluoropyrimidine
Antihistamines
Diphenhydramine
Terfenadine 
Astemizole
Other drugs 
Indapamide
Cisapride 

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Appendix 9 
Adverse Event Definitions
Definitions
An adverse event (AE) is: 
 Any untoward medical occurrence in a study subject, regardless of whether or not
considered drug-related. The examples are as follows:
Any clinically significant recurrence or worsening of a pre-existing condition.
 An AE occurring from overdose of a Sponsor study drug whether accidental or intentional
(i.e., a dose higher than that prescribed by a healthcare professional for clinical reasons).
 A treatment-emergent AE is defined as: an AE that occurs after the subject starts treatment
with the IMP.
A pre-existing condition is a clinical condition (including a condition being treated) that is 
diagnosed before the subject signs the Informed Consent Form and that is documented as part of 
the subject’s medical history. 
Note: A procedure is not an AE, but the reason for a procedure may be an AE. 
The diagnosis should be recorded, in preference to listing the individual signs and symptoms. For 
example, symptoms of fever, cough, and elevated white blood cells should be reported as 
pneumonia rather than the individual signs and symptoms. Abnormal laboratory values are 
typically not reported as Aes by themselves, but rather are included with the underlying 
diagnosis. For example, a low serum hemoglobin value associated with gastrointestinal bleeding 
should not be reported separately from the underlying diagnosis of gastrointestinal hemorrhage. 
Reporting of Adverse Events
At each visit, the investigator, or delegate, will determine whether or not any Aes have occurred. 
Non-leading questions such as “How are you feeling today?” or “Have you had any health 
concerns since your last visit?” should be used to elicit the subject to report any possible AEs. If 
any AEs have occurred, they will be recorded in the AE section of the electronic Case Report 
Form (eCRF) and in the subject’s source documents.  
Aes will be collected from the time of informed consent until the final study visit. Aes that are 
related to the investigational drug and continue beyond the normal collection period (i.e., are 
ongoing at the time a subject exits the study) will be followed until resolution or stabilization. 
Assessment of Severity 
The investigator will be asked to provide an assessment of the AE severity using the Common 
Terminology Criteria for Adverse Events (CTCAE) Version 5.0 classification. This study will 

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enroll subjects undergoing elective surgeries requiring general anesthesia using endotracheal 
intubation; however, not all subjects will be healthy volunteers. Elective surgery does not 
necessarily mean optional surgery. Elective means the surgery can be scheduled in advance, and 
it can still be associated with serious conditions. The intent is to exclude emergent surgeries for 
life-threatening conditions, while still including a diverse, heterogenous patient population such 
as ASA-PS III-IV, elderly and morbidly obese subjects with comorbidities. The CTCAE Version 
5 severity classification is as follows: 
 Grade 1: Mild; asymptomatic or mild symptoms; clinical or diagnostic observations only;
intervention not indicated.
 Grade 2: Moderate; minimal, local or noninvasive intervention indicated; limiting age-
appropriate instrumental activity of daily living (preparing meals, shopping for groceries or
clothes, using the telephone, managing money).
 Grade 3: Severe or medically significant but not immediately life-threatening;
hospitalization or prolongation of hospitalization indicated; disabling; limiting self-care
activity of daily living (bathing, dressing and undressing, feeding self, using the toilet, taking
medications, and not bedridden).
 Grade 4: Life-threatening consequences; urgent intervention indicated.
 Grade 5: Death related to AE.
Relationship to Study Treatment 
The investigator will make a determination of the relationship of the AE to the study drug using 
a 4-category system according to the following guidelines: 
Not related: When the AE is definitely caused by the subject’s clinical state, or the study
procedure/conditions. There is no association between the study drug and the reported event;
there is a clear alternative explanation; a causal relationship is non plausible.
 Unlikely Related: Underlying or concurrent disease or other drugs/exposures provide
plausible alternative explanations. Temporal relationship to study drug administration makes
a causal relationship improbable.
 Likely Related: There is a reasonable possibility that the drug caused the AE; the event is
unlikely attributed to underlying or concurrent disease or other drugs/exposures (i.e.,
alternative explanation). There is a reasonable time sequence to administration of the study
drug.
 Definitely Related: A definite causal relationship exists between the drug administration and
the AE; including a plausible time relationship to drug administration, and it cannot be
explained by underlying or concurrent disease or other drugs/exposures; abates upon

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discontinuation of the drug, follows a known or hypothesized cause-effect relationship, and 
(if appropriate) reappears when the drug is reintroduced. 
Action Taken for Adverse Events
The investigator or designee will record the action taken for the AE in the eCRF. Actions taken 
will include: 
 Dose increased: The medication schedule was modified by addition, either by changing the
frequency, strength, or amount.
 Dose not changed: The medication schedule was not changed.
Dose reduced: The medication schedule was modified by subtraction, either by changing the
frequency, strength, or amount.
 Drug interrupted: The medication schedule was modified by temporarily terminating the
prescribed regimen of medication.
 Drug withdrawn: The medication schedule was modified through termination of the
prescribed regimen of medication.
 Not applicable
 Unknown
Follow-up of Adverse Events 
All serious Aes (SAEs) that are ongoing at the time of discontinuation, or that develop prior to 
the final Follow-up Telephone Call, will be followed for 30 days, or until resolution or 
stabilization. 
Serious Adverse Events
An SAE is any AE occurring at any dose that meets 1 or more of the following criteria: 
 Results in death
 Is life-threatening (see below)
 Requires subject hospitalization or prolongation of an existing hospitalization (see below)
 Results in a persistent or significant disability or incapacity (see below)
 Results in a congenital anomaly or birth defect
 Results in an important medical event (see below).
Additionally, important medical events that may not result in death, be life-threatening, or 
require hospitalization may be considered SAEs when, based on appropriate medical judgment, 
they may jeopardize the subject and may require medical or surgical intervention to prevent one 

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of the outcomes listed above. Examples of such events include allergic bronchospasm requiring 
intensive treatment in an emergency room, blood dyscrasias or convulsions that do not require 
hospitalization, or development of drug dependency or drug abuse. 
A life-threatening adverse event is any AE that places the subject at immediate risk of death 
from the event as it occurred. A life-threatening event does not include an event that might have 
caused death had it occurred in a more severe form but that did not create an immediate risk of 
death as it actually occurred. For example, drug-induced hepatitis that resolved without evidence 
of hepatic failure would not be considered life-threatening, even though drug-induced hepatitis 
of a more severe nature can be fatal. Hospitalization is to be considered only as an overnight 
admission.  
Hospitalization or prolongation of a hospitalization is a criterion for considering an AE to be 
serious.  
The following circumstances should not be considered an AE/SAE: 
 Planned hospitalization for the surgery required for study participation.
 Hospitalization or prolongation of hospitalization is part of a routine procedure followed by
the study center (e.g., stent removal after surgery). This should be recorded in the study file.
 Hospitalization for surgical follow-up required as routine standard of care.
In addition, a hospitalization planned before the start of the study for a pre-existing condition that 
has not worsened does not constitute an SAE (e.g., elective hospitalization for a total knee 
replacement due to a pre-existing condition of osteoarthritis of the knee that has not worsened 
during the study).   
Disability is defined as a substantial disruption in a person’s ability to conduct normal life 
functions (i.e., the AE resulted in a significant, persistent, or permanent change, impairment, 
damage, or disruption in the subject’s bodily function/structure, physical activities, or quality of 
life).   
Medical and scientific judgment should be exercised in deciding whether a case is serious in 
those situations where important medical events may not be immediately life-threatening or 
result in death, hospitalization, disability, or incapacity. These include events that may jeopardize 
the subject or may require medical intervention to prevent one or more outcomes listed in the 
definition of serious. Such events should usually be considered as serious. 

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Appendix 10 Clinical Laboratory Evaluations 
The following clinical laboratory analytes will be assessed: 
Chemistry (Chem-20):
Hematology (CBC):
Albumin
Hematocrit
ALP
Hemoglobin
ALT
MCH
AST
MCHC
BUN
MCV
Calcium
Platelet count 
Chloride
RBC count
Cholesterol (TC, LDL, HDL, TG)
WBC count
Creatinine
WBC differential
GGT 
(% & ABS count) 
Glucose 
Basophils
LDH 
Eosinophils
Phosphorus 
Lymphocytes 
Potassium
Monocytes
Sodium 
Neutrophils
Bilirubin (Total and Indirect)
Total CO2 (measured as bicarbonate) 
Hormone:
Total protein 
Urine pregnancy test 
local laboratory 
only (For females only) 
Uric acid 
Serum pregnancy test (For females only) 
TSH*
Complete Urinalysis (UA):
Color and appearance 
pH and specific gravity
Bilirubin 
Glucose 
Ketones 
Leukocytes 
Nitrite 
Occult blood 
Protein 
Microscopic (including RBCs and 
WBCs) 
Abbreviations: ABS = absolute; ALP = alkaline phosphatase; ALT = alanine aminotransferase; AST = aspartate 
aminotransferase; BUN = blood urea nitrogen; CBC = complete blood count; CO2 = carbon dioxide; GGT = gamma 
glutamyl transferase; LDH = lactate dehydrogenase; MCH = mean corpuscular hemoglobin; MCHC = mean 

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corpuscular hemoglobin concentration; MCV = mean corpuscular volume; RBC = red blood cell; WBC = white 
blood cell. 
*TSH test will only be done during the screening period. TSH is only drawn for subjects with known
hypothyroidism and/or on thyroid-hormone replacement treatment (i.e., thyroxine), or subjects suspected to have
thyroid dysfunction based on clinical laboratory and physical exam.

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Appendix 11 Regulatory, Ethical, and Study Oversight Considerations 
Regulatory and Ethical Considerations 
This study will be conducted in accordance with the protocol and with the following: 
 Consensus ethical principles derived from international guidelines including the Declaration
of Helsinki and Council for International Organizations of Medical Sciences International
Ethical Guidelines
 Applicable ICH GCP Guidelines
Applicable laws and regulations.
The protocol, protocol amendments, Informed Consent Form (ICF), Investigator’s Brochure, and 
other relevant documents (e.g., advertisements) must be submitted to an Institutional Review 
Board (IRB)/Ethics Committee (EC) by the investigator and reviewed and approved by the 
IRB/EC before the study is initiated.  
 Any amendments to the protocol will require IRB/EC approval before implementation of
changes made to the study design, except for changes necessary to eliminate an immediate
hazard to study participants.
The investigator will be responsible for the following: 
 Providing written summaries of the status of the study to the IRB/EC annually or more
frequently in accordance with the requirements, policies, and procedures established by the
IRB/EC
 Notifying the IRB/EC of serious adverse events or other significant safety findings as
required by IRB/EC procedures
Providing oversight of the conduct of the study at the site and adherence to requirements of
21 Code of Federal Regulations (CFR), ICH guidelines, the IRB/EC, European regulation
536/2014 for clinical studies (if applicable), and all other applicable local regulations
Finances and Insurance
Financing and insurance will be addressed in a separate agreement. 
Informed Consent
Prior to starting participation in the study, each subject will be provided with a study-specific 
ICF giving details of the study drugs, procedures, and potential risks of the study. Subjects will 
be instructed that they are free to obtain further information from the investigator (or designee) 
and that their participation is voluntary, and they are free to withdraw from the study at any time. 

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Subjects will be given an opportunity to ask questions about the study prior to providing consent 
for participation.   
Subjects or their legally authorized representative will be required to sign a statement of 
informed consent that meets the requirements of local regulations, ICH guidelines, and the 
IRB/EC or study center, where applicable. The subject will be given a copy of the signed ICF, 
and the original will be maintained with the subject’s records.  
Subjects must be re-consented to the most current version of the ICF(s) during their participation 
in the study. 
Subject Data Protection 
Subjects will be assigned a unique identifier and will not be identified by name in electronic 
Case Report Forms (eCRFs), study-related forms, study reports, or any related publications. 
Subject and investigator personal data will be treated in compliance with all applicable laws and 
regulations. In the event the study protocol, study report, or study data are included in a public 
registry, all identifiable information from individual subjects or investigators will be redacted 
according to applicable laws and regulations.  
The subject must be informed that his/her personal study-related data will be used by the 
Sponsor in accordance with local data protection law. The level of disclosure must also be 
explained to the subject. The subject must also be informed that his/her medical records may be 
examined by Sponsor or Contract Research Organization (CRO) auditors or other authorized 
personnel appointed by the Sponsor, by appropriate IRB/EC members, and by inspectors from 
regulatory authorities. 
Disclosure 
All information provided regarding the study, as well as all information collected and/or 
documented during the course of the study, will be regarded as confidential. The investigator (or 
designee) agrees not to disclose such information in any way without prior written permission 
from the Sponsor. 
Data Quality Assurance 
The following data quality steps will be implemented: 
 All subject data relating to the study will be recorded on eCRFs unless directly transmitted
to the Sponsor or designee electronically (e.g., laboratory data). The investigator is
responsible for verifying that data entries are accurate and correct by electronically signing
the eCRF.

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 The investigator must maintain accurate documentation (source data) that supports the
information entered in the eCRF.
 The investigator must permit study-related monitoring, audits, IRB/EC review, and
regulatory agency inspections and provide direct access to source data documents.
 The Sponsor or designee is responsible for the data management of this study including
quality checking of the data. Pre-defined, agreed risks, monitoring thresholds, quality
tolerance thresholds, controls, and mitigation plans will be documented in a risk
management register. Additional details of quality checking to be performed on the data may
be included in a Data Management Plan.
 Study monitors will perform ongoing source data verification to confirm that data entered
into the eCRF by authorized site personnel are accurate, complete, and verifiable from
source documents; that the safety and rights of subjects are being protected; and that the
study is being conducted in accordance with the currently approved protocol and any other
study agreements, ICH GCP, and all applicable regulatory requirements.
 Records and documents, including signed ICFs, pertaining to the conduct of this study must
be retained by the investigator in accordance with 21 CFR 312.6I) unless local regulations or
institutional policies require a longer retention period. No records may be destroyed during
the retention period without the written approval of the Sponsor. No records may be
transferred to another location or party without written notification to the Sponsor.
Investigator Documentation Responsibilities 
All individual, subject-specific study data will be entered into a 21 CFR Part 11-compliant 
electronic data capture (EDC) system on an eCRF in a timely fashion. All data generated from 
external sources (e.g., central laboratory, pharmacokinetics, pharmacodynamics, 
electrocardiogram central readers) and transmitted to the Sponsor or designee electronically will 
be integrated with the subject’s eCRF data in accordance with the Data Management Plan. 
An eCRF must be completed for each subject who signs an ICF and undergoes any pre-screening 
or screening procedures, according to the eCRF completion instructions. The Sponsor, or CRO, 
will review the supporting source documentation against the data entered into the eCRFs to 
verify the accuracy of the electronic data. The investigator will ensure that corrections are made 
to the eCRFs and that data queries are resolved in a timely fashion by the study staff.   
The investigator will sign and date the eCRF via the EDC system’s electronic signature 
procedure. These signatures will indicate that the investigator reviewed and approved the data on 
the eCRF, the data queries, and the site notifications. 

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Publications
If on completion of the study the data warrant publication, the investigator may publish the 
results in recognized (referred) scientific journals subject to the provisions of the clinical study 
agreement (CSA). Unless otherwise specified in the CSA, the following process shall occur: 
The institution and investigator shall not publish or present data from an individual study center 
until the complete multicenter study has been presented in full or for 2 years after the termination 
of the multicenter study, whichever occurs first. Subsequent publications must refer to the 
multicenter findings. Thereafter, if the investigator expects to participate in the publication of 
data generated from this site, the institution and investigator shall submit reports, abstracts, 
manuscripts, and/or other presentation materials to the Sponsor for review before submission for 
publication or presentation. The Sponsor shall have 60 days to respond with any requested 
revisions, including (without limitation) the deletion of confidential information. The 
investigator shall act in good faith upon requested revisions, except the investigator shall delete 
any confidential information from such proposed publications. The investigator shall delay 
submission of such publication or presentation materials for up to an additional 90 days to have a 
patent application(s) filed.

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Appendix 13
IMP/AxMP designation 
Active 
Substance 
Product Name 
IMP/AxMP 
Designation 
Marketing Authorization 
Status in EEA
Used within Marketing 
Authorization
HSK3486 
HSK3486 2.5 mg/ml injectable 
emulsion 20 ml vial 
IMP (test product) 
No 
N/A
Propofol 
Propofol 10 mg/ml injectable 
emulsion 20 ml vial
IMP (comparator) 
AxMP (rescue
medicine) 
Authorized 
Yes 
Midazolam 
Midazolam solution for 
injection/infusion
AxMP (background 
therapy) 
Authorized 
Yes 
Fentanyl 
Fentanyl solution for injection 
AxMP (background 
therapy) 
Authorized 
Yes 
Rocuronium 
Rocuronium solution for 
injection/infusion
AxMP (background 
therapy) 
Authorized
Yes
Sevoflurane
Sevoflurane inhalation vapour, 
liquid
AxMP (background 
therapy) 
Authorized
Yes
IMP = Investigational Medicinal Product; AxMP = Auxiliary Medicinal Product; EEA = European Economic Area

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Appendix 14 Detailed Summary of Changes 
The below reflects changes made from protocol Version 8.0 to Version 9.0. Minor clarifications, formatting, grammatical and 
spelling errors have been corrected throughout Version 9.0 as needed and may not be outlined below. Corresponding updates 
have been reflected in the synopsis, appendices and figures as applicable.  
Section (s) affected
4.1 Inclusion Criteria
Existing text
9. Patients with psychiatric/mental disorders must be considered stable on treatment (e.g., SSRIs, SNRIs, TCAs, MAOIs,
psychotherapy) and no hospitalization and urgent care for at least 1 year.
Updated text 
9. Patients with psychiatric/mental disorders must be considered stable on treatment (e.g., SSRIs, SNRIs, TCAs, MAOIs,
psychotherapy) per investigator judgement, and no hospitalization and urgent care due to the underlying psychiatric
pathology for at least 12 months.
Section (s) affected
4.2 Exclusion Criteria
Existing text
3. d). Patients with psychiatric/mental disorders who have not been on a stable treatment regimen (e.g., SSRIs, SNRIs,
TCAs, MAOIs, psychotherapy) for at least 1 year or who have been hospitalized or had emergent/urgent care within the
past year.
Updated text 

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3. d). Patients with psychiatric/mental disorders who have not been on a stable treatment regimen (e.g., SSRIs, SNRIs,
TCAs, MAOIs, psychotherapy) per investigator judgement, for at least 12 months or who have been hospitalized or had
emergent/urgent care due to the underlying psychiatric pathology within the last 12 months.
Section (s) affected 
7.3.2 Reporting Serious Adverse Events 
Existing text 
Serious Adverse Event Reporting Contact Information: 
Rho, Inc. Safety Group Email: rho_productsafety@rhoworld.com 
Serious Adverse Event Help Line: 1-888-746-7231 
Serious Adverse Event Fax Line: 1-888-746-3293 
Medical Monitor Contact Information:
Rho, Inc. Medical Monitor           
Anna Pinsky, MD 
Telephone:  919-595-6461 (Medical Monitor Line) 
Email: MedicalMonitorSupport@rhoworld.com AND jamie_chang@rhoworld.com
Updated text 
Serious Adverse Event Reporting Contact Information:
Rho, Inc. Safety Group Email: rho_productsafety@rhoworld.com
Serious Adverse Event Help Line: 1-888-746-7231 
Serious Adverse Event Fax Line: 1-888-746-3293 
Medical Monitor Contact Information:

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Rho, Inc. Medical Monitor           
Anna Pinsky, MD 
Telephone:  919-595-6461 (Medical Monitor Line) 
Email: MedicalMonitorSupport@rhoworld.com AND Anna_Pinsky@rhoworld.com
Section (s) affected 
7.1.2 Day of Surgery (Day 1) 
Existing text 
Administration of study drug must be initiated within 5 minutes [±30 seconds] post midazolam preinduction medication 
administration stop time.
Updated text 
Administration of study drug must be initiated at 5 minutes [±30 seconds] post midazolam preinduction medication 
administration stop time.