Comparison of the Effects of Vaginal Cuff Closure Techniques on Vaginal Length and Sexual Function in Laparoscopic Hysterectomy
Conditions: Uterus Absent
Sponsor: Acibadem Kent Hospital

CLINICAL RESEARCH DOCUMENT – COVER PAGE 
Official Study Title
Comparison of the Effects of Vaginal Cuff 
Closure Techniques on Vaginal Length and 
Sexual Function in Laparoscopic 
Hysterectomy
Document Date
01/05/2022
  STUDY PROTOCOL 
Patients who underwent laparoscopic hysterectomy at the Obstetrics and Gynecology 
Department of Abant İzzet Baysal University's Faculty of Medicine will be included in this study. 
During total laparoscopic hysterectomy, patients will be divided into two groups: those who 
underwent laparoscopic and vaginal cuff closure, based on the techniques used. Patients were 
recorded and evaluated based on their age, gender, parity, body mass index, complaints at 
presentation to our clinic, ultrasonography findings, probe curettage results, smear results, surgical 
indications, current chronic diseases, and previous surgical history. 
The sample size was determined using a repeated-measures ANOVA test using the G*Power
3.1.9.4 program. Accordingly, the minimum sample size to be included with a medium effect size 
(f=0.20), 80% statistical power, and a significance level of 0.05 was determined to be 52. To protect
the study from potential losses during follow-up, an additional 10% was used to calculate 30 
participants in each patient group. 
Demographic data and preoperative vaginal lengths were recorded during the preoperative 
period. Three months after the hysterectomy, vaginal lengths were measured, and sexual life and 
urogenital function were evaluated. Vaginal lengths were measured without anesthesia, using the C 
point used for POP-Q measurement as an example. Under abdominal ultrasound control, a 5-12 
mHz linear probe was used. A rigid Hegar bougie was inserted into the vagina, extending the Hegar 
bougie as far as it would go, marking the area externally, and measuring the length of the penis up 
to the hymen caruncle. This measurement was recorded as the posthysterectomy vaginal length.  
The Arizona Sexual Experiences Scale is a 6-point Likert-type scale consisting of 5 questions. The 
lowest possible score is 5, and the highest is 30. Scores of 11 or more are considered sexual 
dysfunction. 
STATİSTİCAL METHODS 
Data obtained in the study will be analyzed using SPSS. Patients will be divided into two groups: 
laparoscopic and vaginal cuff closures, based on the techniques used during total laparoscopic 
hysterectomy. Power Analysis will be conducted using G Power 3.1.9.4. Statistical analyses will 
utilize descriptive statistics such as number, percentage, standard deviation, mean, and min/max. 
Student's t-test and partial correlation will be used if the data are normally distributed. The chi-
square test will be used to evaluate categorical data. If the data are not normally distributed, the 
Mann-Whitnet U test will be used. A p<0.05 level of significance will be considered. Statistical 
tests will be performed using a statistical program or programs compatible with Windows.