An Extended Access Program to Assess Long-term Safety of Bardoxolone Methyl in Patients With Pulmonary Hypertension
Phase: PHASE3
Conditions: Pulmonary Hypertension
Sponsor: Biogen

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BARDOXOLONE METHYL (RTA 402) 
402-C-1602
 
EUDRACT NUMBER: 2016-004365-16 
AN EXTENDED ACCESS PROGRAM TO ASSESS 
LONG-TERM SAFETY OF BARDOXOLONE METHYL 
IN PATIENTS WITH PULMONARY HYPERTENSION 
VERSION 3.2 – 11 JULY 2019 
GERMANY 
Protocol History 
Version 1.0 – 27 October 2016 
Version 2.0 – 05 January 2017 
Version 2.2 – 23 June 2017 
Version 3.1 – 16 July 2018 
The information contained herein is confidential and the proprietary property of 
Reata Pharmaceuticals, Inc. and any unauthorized use or disclosure of such 
information without the prior written authorization of Reata Pharmaceuticals, 
Inc. is expressly prohibited. 

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INVESTIGATOR’S AGREEMENT 
 
I have received and read the Investigator’s Brochure for bardoxolone methyl.  I have read the 
402-C-1602 clinical study protocol and agree to conduct the study as outlined.  I agree to 
maintain the confidentiality of all information received or developed in connection with this 
protocol.  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Printed Name of Investigator 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Signature of Investigator 
 
 
 
 
 
 
 
 
Date 

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PROCEDURES IN CASE OF EMERGENCY 
 
Table 1: 
Emergency Contact Information 
Role in Study 
Name 
Address and Telephone Number 
Medical and Scientific 
Leader 
 
 
 
 
 
 
 
 
Clinical Study 
Manager 
 
 
 
 
 
 
Medical Monitor 
 
 
 
 
SAE Reporting 
 
 
 
 
 
 
 
 
 
 
Non-North American sites will use access codes specific to their country.  
Numbers for non-North American sites for SAE reporting will be provided 
by the clinical team directly to the sites for reporting and submitting SAE 
information to 
.  
 
 

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2. 
SYNOPSIS 
Name of Sponsor/Company: 
Reata Pharmaceuticals, Inc. 
Name of Investigational Product: 
Bardoxolone methyl 
Title of Study:  
An Extended Access Program to Assess Long-Term Safety of Bardoxolone Methyl in Patients with 
Pulmonary Hypertension  
Study center(s): Approximately 150 study centers  
Studied period: 60 months 
Estimated date first patient enrolled in Germany:  
July 2017 
Estimated date last patient completed in Germany: July 2023 
The study duration may be extended beyond 60 months in a 
future protocol amendment subject to all applicable 
regulatory and ethics approvals.  
The end of the study is defined as the last visit of the last 
patient. 
Phase of development:  
3a 
Primary Objectives:   
To provide continuing open-label treatment with bardoxolone methyl as part of this extended access 
program while collecting ongoing safety and tolerability data of bardoxolone methyl.   
Primary Safety Endpoint: 
Frequency, intensity, and relationship to study drug of adverse events (AEs) and serious adverse events 
(SAEs), and change from baseline in the following assessments: physical examinations, vital sign 
measurements, B-type natriuretic peptide (BNP), N-terminal pro-brain natriuretic peptide (NT-proBNP), 
and weight. 
Methodology:  
This extended access study will assess the long-term safety and tolerability of bardoxolone methyl in 
qualified patients with pulmonary hypertension (PH) who previously participated in controlled clinical 
studies with bardoxolone methyl.  
Qualified patients will start dosing at 10 mg of bardoxolone methyl every other day, then begin once daily 
dosing at Week 4 (unless contraindicated clinically) and continue the drug for up to 5 years or until 
patient withdrawal, whichever is sooner.  The treatment duration may be extended in a future protocol 
amendment subject to all applicable regulatory and ethics approvals.  Dose de-escalation (down to 5 mg) 
is permitted during the study, if indicated clinically.    
All patients in the study will follow the same visit and assessment schedule.  Patients will be scheduled to 
be assessed in person during treatment at Day 1, Week 2, Week 4, Week 6, Week 8, Week 24, and every 
24 weeks thereafter.  Patients will also be assessed by telephone contact on Days 3, 10, 21, 31, and 38.  
Day 1 for this extended access study should occur on the same day as the last visit in the controlled 
clinical study.  Patient status should be carefully monitored by the investigator during the first 8 weeks of 
study treatment.  Assessments required for both the last visit in the controlled study and Day 1 for this 
study should only be completed once. 

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Patients from the CATALYST study: 
Day 1 of this extended access study will coincide with the Week 28 follow-up visit for patients from 
CATALYST (402-C-1504, NCT02657356, EudraCT 2016-000196-24).  If this extended access program 
is not yet open for enrollment at the time of a patient’s Week 28 visit, the Day 1 visit (i.e., enrollment) 
must occur within 30 days of site activation.  Exceptions allowing CATALYST patients who have 
completed 24 weeks of treatment to schedule their follow-up assessments prior to Week 28 and still be 
considered eligible for this open-label study should be discussed with the Chief Medical Officer at Reata, 
or designee, and will be considered on a case-by-case basis.  
Patients from the LARIAT study:  
Day 1 of this extended access study will coincide with the End-of-Study visit for patients from LARIAT 
(402-C-1302, NCT02036970).  
Schema for Study of Bardoxolone Methyl in Patients with Pulmonary Hypertension 
 
 
Number of patients (planned):  
Patient numbers will be determined by those who are treatment-compliant and who have previously 
participated in clinical studies with bardoxolone methyl.  
Main criteria for inclusion: 
1. Treatment-compliant patients who are participating in qualifying ongoing studies (CATALYST, 
EudraCT 2016-000196-24; LARIAT, EudraCT 2016-004793-17) and who have completed 
required End-of-Treatment and/or Follow-up visits in one of these prior clinical studies with 
bardoxolone methyl and who, according to the assessment of the investigator, have a potential 
positive benefit-risk assessment for participating in the trial.  
2. Evidence of a personally signed and dated informed consent document indicating that the patient 
has been informed of all pertinent aspects of the study prior to initiation of any protocol-
mandated procedures. 
Major exclusion criteria: 
1. Participation in other investigational clinical studies involving interventional products being 
tested or used in a way different from the approved form or when used for an unapproved 
indication; 
2. Patients who have an ongoing SAE from a clinical study that is assessed by the investigator as 
related to bardoxolone methyl; 
3. Unwilling to practice acceptable methods of birth control (both males who have partners of 
childbearing potential and females of childbearing potential) while taking study drug;  

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4. Women who are pregnant or breastfeeding; 
5. Patient is, in the opinion of the investigator, unable to comply with the requirements of the study 
protocol or is unsuitable for the study for any reason; 
6. Known hypersensitivity to any component of the study drug. 
Investigational product, dosage, mode of administration, and duration of treatment:  
Bardoxolone methyl will be administered orally (every other day or once daily) at 10 mg for up to 
5 years.  The treatment duration may be extended in a future protocol amendment subject to all applicable 
regulatory and ethics approvals.  Dose de-escalation (down to 5 mg) is permitted during the study, if 
indicated clinically. 
Reference therapy, dosage and mode of administration:  
None. 

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Criteria for evaluation: 
Safety: Results of physical examinations, laboratory results, BNP, NT-ProBNP, vital sign measurements, 
weight, AEs, and SAEs. 
Statistical methods: 
Sample size: The aim of this long-term extended access study is primarily to provide continuing treatment 
to patients with bardoxolone methyl and to assess long-term safety and tolerability, hence no single 
primary variable has been identified.  Patient numbers will be determined by those who have been 
treatment-compliant and who have previously participated in clinical studies with bardoxolone methyl. 
Primary analysis of safety: As the extension is of an open-label design with no comparator group, all 
statistical analyses will be descriptive.  The summary tables will be presented for the overall group of 
patients, and also split by previous treatment groups (i.e., bardoxolone methyl or placebo) in prior 
bardoxolone methyl clinical studies.  

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3. 
TABLE OF CONTENTS AND LIST OF TABLES  
TABLE OF CONTENTS 
1. 
TITLE PAGE ................................................................................................................1 
2. 
SYNOPSIS ...................................................................................................................5 
3. 
TABLE OF CONTENTS AND LIST OF TABLES ....................................................9 
4. 
LIST OF ABBREVIATIONS AND DEFINITIONS OF TERMS.............................14 
5. 
INTRODUCTION ......................................................................................................16 
5.1. 
Study Rationale ...........................................................................................................18 
5.2. 
Clinical Experience with Bardoxolone Methyl ..........................................................18 
5.2.1. 
Safety and Tolerability ...............................................................................................18 
5.2.2. 
Fluid Overload ............................................................................................................18 
5.2.3. 
Transaminase and Gamma-Glutamyl Transpeptidase Elevations ..............................19 
5.2.4. 
Muscle Spasms ...........................................................................................................19 
5.2.5. 
Weight Loss ................................................................................................................20 
5.2.6. 
Hypomagnesaemia ......................................................................................................20 
6. 
STUDY OBJECTIVES AND ENDPOINTS ..............................................................22 
6.1. 
Primary Objectives .....................................................................................................22 
6.2. 
Primary Safety Endpoint: ...........................................................................................22 
7. 
INVESTIGATIONAL PLAN .....................................................................................23 
7.1. 
Overall Study Design ..................................................................................................23 
7.2. 
Number of Patients .....................................................................................................23 
7.3. 
Treatment Assignment ................................................................................................23 
7.3.1. 
Dose Escalation and Dose De-Escalation ...................................................................24 
7.4. 
Criteria for Study Termination ...................................................................................24 
7.5. 
Criteria for Premature Termination or Suspension of Investigational Sites ...............24 
7.6. 
Procedures for Premature Termination or Suspension of the Study or the 
Participation of Investigational Sites ..........................................................................24 
7.7. 
Schedule of Assessments ............................................................................................24 
8. 
SELECTION AND WITHDRAWAL OF PATIENTS ..............................................27 
8.1. 
Patient Inclusion Criteria ............................................................................................27 
8.2. 
Patient Exclusion Criteria ...........................................................................................27 

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8.3. 
Patient Discontinuation and Termination ...................................................................27 
9. 
TREATMENT OF PATIENTS ..................................................................................29 
9.1. 
Select Management Guidelines ..................................................................................29 
9.1.1. 
Management of Fluid Status .......................................................................................29 
9.1.2. 
Management of Elevated Transaminase Levels (ALT and/or AST) ..........................29 
9.1.3. 
Management of Muscle Spasms .................................................................................30 
9.1.4. 
Weight Loss ................................................................................................................30 
9.1.5. 
Hypomagnesaemia ......................................................................................................30 
9.1.6. 
Nausea .........................................................................................................................30 
9.2. 
Description of Study Drug ..........................................................................................31 
9.3. 
Concomitant Medications ...........................................................................................31 
9.3.1. 
Excluded Medications ................................................................................................31 
9.3.2. 
Permitted Medications ................................................................................................31 
9.4. 
Treatment Compliance ................................................................................................32 
9.5. 
Randomization ............................................................................................................32 
9.6. 
Blinding ......................................................................................................................32 
9.7. 
Unscheduled Visits .....................................................................................................32 
9.8. 
Pregnancy ...................................................................................................................32 
9.8.1. 
Women of Childbearing Potential ..............................................................................32 
9.8.2. 
Methods of Birth Control............................................................................................32 
9.8.3. 
Suspected Pregnancy ..................................................................................................33 
9.9. 
Serious Toxicities .......................................................................................................33 
9.10. 
Study Procedures ........................................................................................................34 
9.10.1. 
Informed Consent .......................................................................................................34 
9.10.2. 
Inclusion/Exclusion ....................................................................................................34 
9.10.3. 
Prior and Current Concomitant Medications ..............................................................34 
9.10.4. 
Current PAH Background Medications ......................................................................34 
9.10.5. 
Weight and Body Mass Index .....................................................................................34 
9.10.6. 
Vital Sign Measurements ............................................................................................34 
9.10.7. 
Physical Examination .................................................................................................35 
9.10.8. 
Pregnancy Test ............................................................................................................35 
9.10.9. 
Study Drug Administration.........................................................................................35 
9.10.10. 
Study Drug Dispensation and Collection ...................................................................35 

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9.10.11. 
Telephone Contact ......................................................................................................35 
9.10.12. 
Adverse Event Collection ...........................................................................................35 
9.10.13. 
Clinical Chemistry ......................................................................................................36 
9.10.14. 
N-Terminal Pro-Brain Natriuretic Peptide and Brain Natriuretic Peptide .................36 
9.10.15. 
6-Minute Walk Test ....................................................................................................36 
9.10.16. 
WHO/NYHA Functional Class Assessment ..............................................................36 
10. 
STUDY DRUG MATERIALS AND MANAGEMENT ...........................................37 
10.1. 
Study Drug ..................................................................................................................37 
10.2. 
Study Drug Packaging and Labeling ..........................................................................37 
10.3. 
Study Drug Storage .....................................................................................................37 
10.4. 
Study Drug Administration.........................................................................................37 
10.5. 
Study Drug Accountability .........................................................................................38 
10.6. 
Study Drug Handling and Disposal ............................................................................38 
11. 
SAFETY ASSESSMENTS ........................................................................................39 
11.1. 
Safety Parameters .......................................................................................................39 
11.2. 
Adverse and Serious Adverse Events .........................................................................39 
11.2.1. 
Definition of Adverse Events .....................................................................................39 
11.2.1.1. 
Adverse Event .............................................................................................................39 
11.2.1.2. 
Serious Adverse Event ................................................................................................39 
11.3. 
Eliciting Adverse Event Information ..........................................................................40 
11.4. 
Assessment of Causality .............................................................................................41 
11.5. 
Assessment of Severity ...............................................................................................41 
11.6. 
Recording Adverse Events .........................................................................................41 
11.7. 
Reporting Serious Adverse Events .............................................................................42 
12. 
STATISTICS ..............................................................................................................45 
12.1. 
Sample Size ................................................................................................................45 
12.2. 
Study Variables ...........................................................................................................45 
12.2.1. 
Safety Variables ..........................................................................................................45 
12.3. 
Statistical Analyses .....................................................................................................45 
12.3.1. 
Primary Analysis of Safety .........................................................................................45 
13. 
DIRECT ACCESS TO SOURCE DATA/DOCUMENTS.........................................46 
13.1. 
Study-Specific Committees ........................................................................................46 
13.2. 
Study Monitoring ........................................................................................................46 

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13.3. 
Audits and Inspections ................................................................................................46 
14. 
QUALITY CONTROL AND QUALITY ASSURANCE .........................................47 
14.1. 
Quality Assurance .......................................................................................................47 
14.2. 
Financial Disclosure ...................................................................................................47 
14.3. 
Sponsor Obligations....................................................................................................47 
14.4. 
Investigator Documentation ........................................................................................47 
14.5. 
Clinical Study Insurance .............................................................................................48 
14.6. 
Use of Information ......................................................................................................48 
15. 
ETHICS ......................................................................................................................49 
15.1. 
Institutional Review Board or Ethics Committee Review ..........................................49 
15.2. 
Ethical Conduct of the Study ......................................................................................49 
15.3. 
Written Informed Consent ..........................................................................................49 
15.4. 
Confidentiality ............................................................................................................50 
15.5. 
Modification of the Protocol .......................................................................................50 
15.6. 
Protocol Deviations ....................................................................................................51 
16. 
DATA HANDLING AND RECORDKEEPING .......................................................52 
16.1. 
Retention of Records ..................................................................................................52 
16.2. 
Case Report Forms .....................................................................................................52 
17. 
PUBLICATION POLICY ..........................................................................................53 
18. 
REFERENCES ...........................................................................................................54 
19. 
APPENDIX 1: ATS GUIDELINES FOR THE SIX-MINUTE WALK TEST ..........56 
20. 
APPENDIX 2: FUNCTIONAL CLASSIFICATION OF PULMONARY 
HYPERTENSION MODIFIED AFTER THE NEW YORK HEART 
ASSOCIATION FUNCTIONAL CLASSIFICATION ACCORDING TO 
THE WHO 1998 (GALIE, 2009) ...............................................................................64 
21. 
APPENDIX 3:  BORG CR10 SCALE .......................................................................65 
 

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LIST OF TABLES 
Table 1: 
Emergency Contact Information ...................................................................................4 
Table 2: 
Abbreviations and Specialist Terms ...........................................................................14 
Table 3: 
Schedule of Assessments ............................................................................................25 
Table 4: 
Bardoxolone Methyl Drug Product Information ........................................................31 
Table 5: 
SAE Reporting Contact Information ..........................................................................43 
 

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4. 
LIST OF ABBREVIATIONS AND DEFINITIONS OF TERMS 
The following abbreviations and specialist terms are used in this study protocol. 
Table 2: 
Abbreviations and Specialist Terms 
Abbreviation or Specialist Term 
Explanation 
6MWD 
6-minute walk distance 
6MWT 
6-minute walk test 
AE 
Adverse event 
ALT 
Alanine aminotransferase 
AST 
Aspartate aminotransferase 
ATP 
Adenosine triphosphate 
ATS 
American Thoracic Society 
BMI 
Body mass index 
BNP 
B-type natriuretic peptide 
CFR 
Code of Federal Regulations (US) 
CK 
Creatine kinase 
CKD 
Chronic kidney disease 
CRO 
Contract research organization 
CTD-PAH 
Connective tissue disease-associated pulmonary arterial 
hypertension 
EC 
Ethics Committee 
eCRF 
Electronic case report form 
eGFR 
Estimated glomerular filtration rate 
FDA 
Food and Drug Administration (US) 
GCP 
Good Clinical Practice 
GGT 
Gamma-glutamyl transpeptidase 
ICH 
International Conference on Harmonization 
IKKβ 
Inhibitor of nuclear factor kappa β kinase beta subunit 
ILD 
Interstitial lung disease 
INR 
International normalized ratio 
IRB 
Institutional Review Board 
Nrf2 
Nuclear factor (erythroid-derived 2)-related factor 2 
NT-Pro BNP 
N-Terminal Pro-Brain Natriuretic Peptide   

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Abbreviation or Specialist Term 
Explanation 
NYHA FC 
New York Heart Association Functional Classification 
PAH 
Pulmonary arterial hypertension 
PH 
Pulmonary hypertension 
ROS 
Reactive oxygen species 
SAE 
Serious adverse event 
SAP 
Statistical analysis plan 
TBL 
Total bilirubin 
ULN 
Upper limit of normal 
US 
United States 
WHO 
World Health Organization 
WOCBP 
Women of child bearing potential 
 

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5. 
INTRODUCTION 
Bardoxolone methyl and its analogs are oleanolic acid-derived synthetic triterpenoid compounds 
that potently induce the Nrf2-Keap1 pathway (Wu, 2011; Rojas-Rivera, 2012).  Through 
interaction with the Nrf2 repressor molecule, Keap1, bardoxolone methyl and its analogs 
promote translocation of Nrf2 to the nucleus, where Nrf2 binds to antioxidant response elements 
in the promoter region of its target genes, leading to induction of many antioxidant and 
cytoprotective enzymes and related proteins (Lee, 2009; Dinkova-Kostova, 2005).  Bardoxolone 
methyl and its analogs are also potent inhibitors of the NF-κB inflammatory pathway through 
both direct (i.e., inhibition of IKKβ kinase activity) and indirect mechanisms (i.e., detoxification 
of reactive oxygen species [ROS]) (Osburn, 2008).  Because of this dual mechanism of action, 
bardoxolone methyl and its analogs are hypothesized to have potential therapeutic relevance in a 
variety of disease settings involving oxidative stress and inflammation.  Activation of the Nrf2 
pathway also transcriptionally regulates multiple genes that promote the production of cellular 
energy within the mitochondria and facilitates mitochondrial homeostasis and efficiency.   
Through induction of Nrf2 and suppression of NF-κB, bardoxolone methyl has broad molecular 
and pharmacological effects that address multiple facets of the pathophysiology of pulmonary 
arterial hypertension (PAH).  Induction of NF-κB is markedly elevated in macrophages, 
endothelial cells and smooth muscle cells of PAH patients (Price, 2010).  Recent evidence 
demonstrates that genetic or pharmacologic induction of Nrf2 suppresses formation of the PAH 
phenotype in a mouse model of hypoxic pulmonary hypertension (PH) (Eba, 2013).   
Additionally, an emerging concept in the pathogenesis of PH is the role of systemic metabolic 
and skeletal muscle dysfunction as a major cause of reduced functional capacity and fatigue, as 
well as disease progression.  A high frequency of insulin resistance and metabolic syndrome-like 
features have been described in patients with PH, and glucose intolerance is associated with a 
decrease in 6-minute walk distance (6MWD) (Pugh, 2011).  Pulmonary hypertension patients 
have decreased adenosine triphosphate (ATP) production (Sutendra, 2014), which results in 
skeletal muscle dysfunction and impaired functional capacity (Mainguy, 2010; Batt, 2014).  
Indeed, the skeletal muscle within patients with PH displays a decreased expression of 
mitochondrial respiration enzymes, defects in mitochondrial oxidative phosphorylation, and 
impairments in mitochondrial biogenesis (Batt, 2014; Malenfant, 2015).  Overall, these defects in 
skeletal muscle contribute to muscle weakness and fatigue, as well as impaired exercise capacity 
in patients with PH (Batt, 2014).  
Several lines of evidence suggest that Nrf2 activation can increase mitochondrial respiration and 
biogenesis, in addition to inducing expression of numerous antioxidative genes to counter ROS.  
Specifically, by increasing the availability and production of reducing equivalents such as 
nicotinamide adenine dinucleotide and flavin adenine dinucleotide, Nrf2 activation improves 
mitochondrial ATP production and improves the efficiency of mitochondrial respiration and 
oxygen consumption.  In addition, Nrf2 activation results in improved beta-oxidation of fatty 
acids and improved uptake of glucose, which leads to improvement in mitochondrial respiration, 
oxygen consumption, and energy production.  Since Nrf2 activation can increase mitochondrial 
respiration (Holmström, 2013; Ludtmann, 2014), bardoxolone methyl may restore metabolic 
deficits in PH, thereby acutely improving energy production and 6MWD. 

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patients with CTD-PAH had similar sustained increases in 6MWD through Week 32.  On the 
basis of these data, Reata expanded the LARIAT protocol (Version 5.0) to include PH patients 
with ILD, including WHO Group III patients with CTD-associated ILD, idiopathic pulmonary 
fibrosis, non-specific interstitial pneumonia, and WHO Group V patients with sarcoidosis.  Reata 
has also initiated a Phase 3 study in WHO Group I CTD-PAH patients (402-C-1504, 
CATALYST, NCT02657356, EudraCT 2016-000196-24) to study the safety and efficacy of 
bardoxolone methyl in those patients.   
5.1. 
Study Rationale 
The established pharmacologic effects of bardoxolone methyl, including the suppression of 
pathologic NF-κB signaling and inflammation and mitochondrial dysfunction, are directly 
applicable to the treatment of PH.  Despite available therapies, the prognosis for PH remains 
poor, especially for patients with CTD (Chung, 2014) and ILD.  Given the severity of the 
underlying disease, this study seeks to offer patients who previously participated in clinical 
studies extended access to bardoxolone methyl until it is available through commercial channels 
(including reimbursement).  For sites in Germany, the trial duration is limited to 5 years 
following the enrollment of the first patient in Germany.  The trial duration may be extended in a 
future protocol amendment subject to all applicable regulatory and ethics approvals. 
For further information, refer to the Investigator’s Brochure.  
5.2. 
Clinical Experience with Bardoxolone Methyl 
Approximately 1950 individuals have been exposed to bardoxolone methyl at the time of 
initiation of this study.  Sixteen studies have been completed (7 in patients with chronic kidney 
disease (CKD) who also had type 2 diabetes, 4 in non-CKD indications, and 5 in healthy 
subjects) and 2 studies are ongoing in patients with PH.   
5.2.1. 
Safety and Tolerability 
Please refer to the Investigator’s Brochure for a detailed discussion of safety findings with 
bardoxolone methyl for studies in healthy subjects, oncology, CKD, and patients with PH. 
5.2.2. 
Fluid Overload 
Similar to endothelin receptor antagonists in certain patient populations, including bosentan in 
advanced congestive heart failure and avosentan in advanced CKD, bardoxolone methyl 
treatment was found to be associated with an increased risk for fluid overload and heart failure 
hospitalizations in a Phase 3 trial (BEACON), which enrolled patients with Stage 4 CKD (eGFR 
of 15-29 mL/min/1.73 m2) and type 2 diabetes.  The overall increased risk for fluid overload and 
heart failure events with bardoxolone methyl appeared to be limited to the first three to four 
weeks after initiation of treatment.  Elevated B-type natriuretic peptide (BNP) and prior 
hospitalization for heart failure were identified as risk factors that contributed to increased risk 
for these events.  The increased risk for these events from bardoxolone methyl treatment had not 
been observed in six previous CKD studies, which were conducted mostly in patients with Stage 
3b CKD (eGFR of 30-44 mL/min/1.73 m2), patients with hepatic dysfunction, cancer patients, or 
healthy volunteers.  

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Review of admission notes and narrative descriptions for heart failure hospitalizations in 
BEACON indicates that heart failure in bardoxolone methyl-treated patients was often preceded 
by rapid fluid weight gain (several kilograms within the first weeks of treatment initiation) and 
was not associated with acute renal decompensation or acutely reduced left ventricular 
contractility.  Available data from BEACON and other studies suggest that bardoxolone methyl 
treatment can differentially affect hemodynamic status according to the clinical condition of 
patients and likely promotes fluid retention in patients with more advanced renal dysfunction and 
other recognized risk factors associated with heart failure at baseline.   
In a Phase 2 dose-ranging study of the efficacy and safety of bardoxolone methyl in patients with 
PH (LARIAT), risk mitigation procedures were employed to reduce the potential for 
bardoxolone methyl-induced fluid overload; these procedures excluded patients with the 
identified risk factors and ensured close monitoring for fluid retention within the first month of 
treatment.  To date, the risk for acute fluid overload adverse events (AEs) with bardoxolone 
methyl in late-stage CKD patients has not been observed in PAH patients.   
5.2.3. 
Transaminase and Gamma-Glutamyl Transpeptidase Elevations  
In clinical studies of bardoxolone methyl, almost all patients had increases of transaminase 
enzymes above baseline upon initiation of treatment, which followed a consistent pattern.  These 
increases were not associated with elevations in bilirubin or other signs of liver toxicity.  In a 
large Phase 3 study that enrolled 2185 patients with type 2 diabetes and stage 4 CKD 
(BEACON), fewer hepatobiliary SAEs were observed in the bardoxolone methyl arm than in the 
placebo arm.  The elevations begin immediately after initiation of treatment or an increase in 
dose; they peak approximately two to four weeks later.  In most patients, transaminase elevations 
were mild, but approximately 4% to 11% of patients experienced an elevation greater than 3X 
the upper limit of normal (ULN).  The elevations resolved to levels less than the ULN in most all 
patients with elevations, within two weeks after peak values while patients continued taking 
study drug.  Patients who experienced elevations to greater than 3X the ULN sometimes required 
additional time to resolve.  While some patients have had elevations to above 3X the ULN, 
persistent elevations to above 3X the ULN have not been observed, and the elevations did not 
recur once resolved, unless caused by other factors. 
Bardoxolone methyl regulates gamma-glutamyl transpeptidase (GGT), a known Nrf2 target 
gene.  In clinical studies, low level GGT elevations during treatment were common, mild, and 
typically lasted longer than alanine aminotransferase (ALT)/aspartate aminotransferase (AST) 
elevations.  Bilirubin levels in patients experiencing transaminase or GGT elevations due to 
treatment with bardoxolone methyl either remained at baseline levels or decreased.  The ALT, 
AST, and GGT elevations were generally self-limiting in patients who continued treatment with 
study drug.  
5.2.4. 
Muscle Spasms 
Muscle spasm was the most frequently reported AE in clinical studies of bardoxolone methyl in 
patients with CKD who also had type 2 diabetes.  The muscle spasms most often manifested in 
the first two months of treatment and resolved spontaneously or with empirical treatment.  They 
occurred mostly at night, in the lower extremities, and were generally mild to moderate in 
severity.  Muscle spasms have also been reported in bardoxolone methyl-treated PAH patients 

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but at lower incidences than that observed in prior CKD studies.  Moreover, the incidence of 
muscle spasms is similar to that observed in placebo-treated PAH patients.  Muscle spasms may 
result from improved insulin sensitivity and glucose uptake in skeletal muscle cells.  Increases in 
glucose uptake, as assessed by the hyperinsulinemic-euglycemic clamp procedure, were 
observed in response to bardoxolone methyl in a defined subset of patients enrolled in a Phase 2a 
study.  To date, in those cases where serum creatinine kinase (CK) levels have been measured, 
no association has been observed between muscle spasms and elevated CK levels in patients 
treated with bardoxolone methyl.  Clinical signs and laboratory findings associated with the 
reports of muscle spasms have not been consistent with muscle toxicity.  Bardoxolone methyl 
subjects showed no increase in prominent laboratory findings associated with muscle toxicity, 
such as increased levels of serum markers, including creatinine, lactate dehydrogenase, blood 
urea nitrogen, uric acid, phosphorus, and potassium, which were monitored weekly during the 
first two months of a prior study (BEAM) when muscle spasms were most frequently reported.  
Increases in the whole-body glucose disposal rate have been observed in mice treated with 
bardoxolone methyl, as well.  Increased glucose uptake was observed in isolated calf muscles of 
the mice, but not in white adipose tissue (Saha, 2010).   
5.2.5. 
Weight Loss 
Decreases in weight and reports of anorexia/decreased appetite have been observed following 
treatment with bardoxolone methyl in patients with CKD who also had type 2 diabetes.  In 
studies of these patients, 17% of bardoxolone methyl patients reported AEs of weight decrease or 
decreased appetite (irrespective of relationship to treatment).  Weight reduction was more 
pronounced in patients treated with bardoxolone methyl than in those given placebo. 
Weight loss of approximately one kilogram per month was observed, with patients of higher 
body-mass index at baseline losing more weight (in absolute terms) than those of normal or 
moderately-elevated body-mass index.   
Bardoxolone methyl-treated PAH patients have also had decreases in weight, with mean weight 
decreases of approximately 3 kg versus placebo at Week 16.  Weight loss in PAH patients has 
not coincided with reports of decreased appetite or anorexia AEs. 
5.2.6. 
Hypomagnesaemia 
Hypomagnesaemia has not been reported in PAH patients to date, but was reported as an AE for 
15.5% of patients with CKD who also had type 2 diabetes who received bardoxolone methyl.  
The AE of hypomagnesaemia (of any reported relationship to study drug) was more frequently 
reported in bardoxolone methyl-treated patients than in patients given placebo.  The investigators 
considered almost all reported events to be mild.  Additionally, patients treated with bardoxolone 
methyl had a greater decrease from baseline in serum magnesium levels than patients given 
placebo; the decrease was evident within 4 weeks and attenuated after 8 weeks of starting 
therapy.  In bardoxolone methyl clinical studies performed to date, a post-hoc analysis identified 
no correlation between hypomagnesaemia and either gastrointestinal AEs or cardiac AEs, 
including cardiac dysrhythmias and prolonged QTc.  The 24-hour urine collections from the 
BEACON ambulatory blood pressure monitoring sub-study showed no increase in urinary 
magnesium levels, indicating that renal loss of magnesium did not account for the reductions in 
serum magnesium observed with bardoxolone methyl treatment in CKD patients.  Notably, a 

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thorough QT study that tested doses of bardoxolone methyl up to 80 mg, bardoxolone methyl 
showed no increase in the QT interval.  

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6. 
STUDY OBJECTIVES AND ENDPOINTS 
6.1. 
Primary Objectives 
To provide continuing treatment with bardoxolone methyl as part of this extended access 
program while collecting ongoing safety and tolerability data of bardoxolone methyl.  
6.2. 
Primary Safety Endpoint: 
Frequency, intensity, and relationship to study drug of adverse events (AEs) and serious adverse 
events (SAEs), and change from baseline in the following assessments: physical examinations, 
vital sign measurements, BNP, N-terminal pro-brain natriuretic peptide (NT-proBNP), and 
weight. 

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7. 
INVESTIGATIONAL PLAN 
7.1. 
Overall Study Design 
This extended access study will assess the long-term safety and tolerability of bardoxolone 
methyl in qualified patients with PH who previously participated in controlled clinical studies 
with bardoxolone methyl. 
Qualified patients will start dosing at 10 mg of bardoxolone methyl every other day, then begin 
once daily dosing at Week 4 (unless contraindicated clinically) and continue the drug for up to 
5 years or until patient withdrawal, whichever is sooner.  The treatment duration may be 
extended in a future protocol amendment subject to all applicable regulatory and ethics 
approvals.  Dose de-escalation (down to 5 mg) is permitted during the study if indicated 
clinically.  Refer to Section 7.3.1 for additional details on dose de-escalation.  
All patients in the study will follow the same visit and assessment schedule.  Patients will be 
scheduled to be assessed in person during treatment at Day 1, Week 2, Week 4, Week 6, Week 8, 
Week 24, and every 24 weeks thereafter.  Patients will also be assessed by telephone contact on 
Days 3, 10, 21, 31, and 38.  Day 1 for this extended access study should occur on the same day 
as the last visit in the controlled clinical study.  Patient status should be carefully monitored by 
the investigator during the first 8 weeks of study treatment.  Assessments required for both the 
last visit in the controlled study and Day 1 for this study should only be completed once. 
Patients from the CATALYST study: 
Day 1 of this extended access study will coincide with the Week 28 follow-up visit for patients 
from CATALYST (402-C-1504, NCT02657356, EudraCT 2016-000196-24).  If this extended 
access program is not yet open for enrollment at the time of a patient’s Week 28 visit, the Day 1 
visit (i.e., enrollment) must occur within 30 days of site activation.  Exceptions allowing 
CATALYST patients who have completed 24 weeks of treatment to schedule their follow-up 
assessments prior to Week 28 and still be considered eligible for this open-label study should be 
discussed with the Chief Medical Officer at Reata, or designee, and will be considered on a case-
by-case basis.   
Patients from the LARIAT study: 
Day 1 of this extended access study will coincide with the End-of-Treatment visit for patients 
from LARIAT (402-C-1302, NCT02036970).   
7.2. 
Number of Patients 
Patient numbers will be determined by those who have been treatment-compliant and who have 
previously participated in clinical studies with bardoxolone methyl. 
7.3. 
Treatment Assignment 
All patients will receive bardoxolone methyl in this study. 

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7.3.1. 
Dose Escalation and Dose De-Escalation  
Patients will start dosing at 10 mg of bardoxolone methyl every other day, then begin once daily 
dosing at Week 4 unless contraindicated clinically.  The investigator should discuss any reasons 
for not dose-escalating to once daily dosing at Week 4 with the medical monitor. 
Dose de-escalation is permitted during the study and the investigator may choose to decrease the 
patient’s dose to 5 mg if clinically indicated.  Reasons for dose de-escalation should be discussed 
with the medical monitor prior to changing dose.  Once a patient’s dose has been decreased to 
5 mg, they cannot dose-escalate back to 10 mg of bardoxolone methyl. 
Unscheduled visits due to dose de-escalation should include assessments detailed in Section 9.7.  
7.4. 
Criteria for Study Termination 
The Sponsor reserves the right to discontinue the study at any time for clinical or administrative 
reasons, or if required by regulatory agencies.  If the Sponsor discontinues the study, all study 
drugs will be discontinued and the investigator will be responsible for securing any alternative 
therapy to be administered, as appropriate. 
7.5. 
Criteria for Premature Termination or Suspension of Investigational 
Sites 
A study site may be terminated prematurely or suspended if the site (including the investigator) 
is found in significant violation of Good Clinical Practice (GCP), protocol, or contractual 
agreement with Sponsor or the site is unable to ensure adequate performance of the study. 
7.6. 
Procedures for Premature Termination or Suspension of the Study 
or the Participation of Investigational Sites 
In the event that the Sponsor, an institutional review board (IRB)/ethics committee (EC), or 
regulatory authority elects to terminate or suspend the study or the participation of an 
investigational site, a study-specific procedure for early termination or suspension will be 
provided by the Sponsor. 
7.7. 
Schedule of Assessments 
Table 3 lists the overall schedule of assessments for the study. 
 

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Table 3: 
Schedule of Assessments  
Study Week 
(Day) 
Assessment 
Day 1a 
Week 1  
(Phone) 
Day 3±2 
Week 1  
Day 7±3 
Week 2  
(Phone) 
Day 10±2 
Week 2 
Day 14±3 
Week 3 
(Phone) 
Day 21±2 
Week 4 
Day 28±3 
Week 4 
(Phone) 
Day 31±2 
Week 5  
(Phone) 
 Day 38±2 
Week 6 
Day 42±3 
Week 8 
Day 56±3 
Week 
24±3,  
Every 24 
Weeks  
End of  
Study 
 Visitb 
Informed consent 
X 
 
 
 
 
 
 
 
 
 
 
 
 
Inclusion/Exclusion 
X 
 
 
 
 
 
 
 
 
 
 
 
 
Prior and Concomitant 
medications 
Xc 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
Current PAH background 
medications 
Xc 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
Weight at home 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
 
 
Weight in clinic  
Xc 
 
X 
 
X 
 
X 
 
 
X 
X 
X 
X 
Vital sign measurements 
Xc 
 
X 
 
X 
 
X 
 
 
X 
X 
X 
X 
Physical examination 
Xc 
 
X 
 
X 
 
X 
 
 
X 
X 
X 
X 
Pregnancy testd 
Xc 
 
 
 
 
 
 
 
 
 
 
X 
 
Dispense study drug 
X 
 
 
 
 
 
X 
 
 
 
 
X 
 
Collect study drug 
 
 
 
 
 
 
X 
 
 
 
 
X 
X 
Telephone contact 
 
X 
 
X 
 
X 
 
X 
X 
 
 
 
 
Adverse event collection 
Xe 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
X 
Clinical Chemistriesf 
Xc 
 
X 
 
X 
 
X 
 
 
X 
X 
X 
X 
BNP and/or NT-Pro BNP 
Xc 
 
X 
 
X 
 
X 
 
 
X 
X 
X 
X 
6-min walk test 
Xc 
 
 
 
 
 
 
 
 
 
 
X 
X 
Dyspnea and fatigueg 
Xc 
 
 
 
 
 
 
 
 
 
 
X 
X 
WHO functional class 
Xc 
 
 
 
 
 
 
 
 
 
 
X 
X 
a On Day 1, all procedures should be performed before study drug administration for patients who are treatment-compliant and have previously participated in 
clinical studies with bardoxolone methyl. 
b After the Week 24 visit, patients will continue to be assessed in person every 24 weeks.  The End of  Study visit should occur within 30 days after the patient 
receives their final dose of study medication under this protocol.  Patients who will be discontinued from the study prior to the completion of the study must 
also complete all End-of-Study assessments. 
c Day 1 assessments should be performed as part of End-of-Treatment or Follow-up visit procedures from a prior study with bardoxolone methyl.  
d A serum pregnancy test will be performed at any point in time if a pregnancy is suspected.  All other pregnancy assessments will be urine pregnancy tests.  
Additional pregnancy assessments will be performed more frequently if required by local law or requested by local health authorities or IRBs/ECs. 

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e AE assessments on Day 1 should be performed following study drug administration. 
f Clinical chemistries will be analyzed locally. 
g Dyspnea and fatigue must be assessed prior to beginning and immediately following completion of the 6MWT using the Borg scale. 
 
Abbreviations: PAH, pulmonary arterial hypertension; BNP, B-type natriuretic peptide; NT-proBNP, N-terminal pro B-type natriuretic peptide; WHO, World 
Health Organization; AE, adverse event; 6MWT, 6-minute walk test.

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8. 
SELECTION AND WITHDRAWAL OF PATIENTS 
8.1. 
Patient Inclusion Criteria 
Main criteria for inclusion:  
1. Treatment-compliant patients who are participating in qualifying ongoing studies 
(CATALYST, EudraCT 2016-000196-24; LARIAT, EudraCT 2016-004793-17) and who 
have completed required End-of-Treatment and/or Follow-up visits in one of these prior 
clinical studies with bardoxolone methyl and who, according to the assessment of the 
investigator, have a potential positive benefit-risk assessment for participating in the trial.   
2. Evidence of a personally signed and dated informed consent document indicating that the 
patient has been informed of all pertinent aspects of the study prior to initiation of any 
protocol-mandated procedures. 
8.2. 
Patient Exclusion Criteria 
All patients with any of the following conditions or characteristics must be excluded from the 
study: 
1. Participation in other investigational clinical studies involving interventional products 
being tested or used in a way different from the approved form or when used for an 
unapproved indication; 
2. Patients who have an ongoing serious adverse event (SAE) from a clinical study that is 
assessed by the investigator as related to bardoxolone methyl; 
3. Unwilling to practice acceptable methods of birth control (both males who have partners 
of childbearing potential and females of childbearing potential) while taking study drug;  
4. Women who are pregnant or breastfeeding; 
5. Patient is, in the opinion of the investigator, unable to comply with the requirements of 
the study protocol or is unsuitable for the study for any reason; 
6. Known hypersensitivity to any component of the study drug. 
8.3. 
Patient Discontinuation and Termination 
Patients have the right to discontinue study drug or withdraw from the study at any time for any 
reason, without prejudice to their medical care.  Furthermore, the investigator may discontinue a 
patient from study drug.  Consultation with the medical monitor should occur prior to study drug 
discontinuation or withdrawing a patient from the study.  The reason for a patient’s 
discontinuation from study drug or study termination will be recorded in the electronic case 
report form (eCRF).  
Discontinuation refers to a patient’s stopping administration of study drug and all study 
assessments and visits.  Reasons for study drug discontinuation may include the following: 

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• Occurrence of an AE or change in medical status that will lead the investigator to be 
concerned about the patient’s welfare; 
• Administrative reasons (e.g., inability to continue); 
• Sponsor termination of the study; 
• Voluntary withdrawal; 
• Females who become pregnant during the study; 
• Premature termination or suspension of an investigational site; 
• Loss to follow-up; 
• Death; 
• Withdrawal of consent. 
Patients must discontinue study drug if any of the following occur. 
• ALT or AST > 8X ULN; 
• ALT or AST > 5X ULN for more than 2 weeks; 
• ALT or AST > 3X ULN and (total bilirubin > 2X ULN or International Normalized 
Ratio [INR] > 1.5); 
• ALT or AST > 3X ULN with the appearance of fatigue, nausea, vomiting, right upper 
quadrant pain or tenderness, fever, rash, and/or eosinophilia (> 5%). 
Patients who discontinue from the study will be asked to complete the End-of-Study procedures 
noted in Table 3. 

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9. 
TREATMENT OF PATIENTS 
9.1. 
Select Management Guidelines 
The following guidelines apply to the management of study participants: 
9.1.1. 
Management of Fluid Status 
Specific risk mitigation procedures will be employed to reduce the potential for bardoxolone 
methyl-induced fluid overload.  Laboratory data, as well as BNP and/or NT-proBNP, will also be 
used to monitor fluid status.   
Additionally, patients will be monitored for rapid weight gain suggestive of fluid overload.  
Patients will be given a Sponsor-provided scale to use at home to monitor their weights daily 
during the first 8 weeks of the treatment period.  Patients who experience a 
5-pound (2.3 kilogram) or greater increase in weight since their Day 1 weight during the first 
8 weeks of the treatment period will be instructed to stop taking their study medication 
immediately and return to the clinic for an unscheduled physical examination and laboratory 
assessment by the investigator.  Patients may not restart their study medication until the 
investigator has completed and documented an assessment of fluid overload.  
Investigators should advise patients to watch for signs and symptoms of fluid overload.  Patients 
should be informed to notify their physicians immediately if they experience swollen feet, chest 
pain, shortness of breath with mild exertion or while lying down, or other relevant symptoms.  
The investigator should immediately assess symptoms of fluid overload and determine 
appropriate medical management, as necessary, including whether stopping drug administration 
will be required.  At the earliest sign of worsening or new onset peripheral edema, or other signs 
and symptoms of acute volume overload, investigators will also be expected to determine if 
changes to a patient’s diuretic regimen is needed. 
9.1.2. 
Management of Elevated Transaminase Levels (ALT and/or AST) 
Transaminases should be monitored following patient’s standard of care.  Nearly all instances of 
elevated transaminases are expected to be asymptomatic.  Transaminase levels (as well as total 
bilirubin [TBL], GGT, alkaline phosphatase, and INR) will be checked within 48 to 72 hours 
during an unscheduled visit if the following occurs: 
• ALT or AST levels > 3X ULN. 
Testing will be repeated every 72 to 96 hours until transaminase levels are below 3X the ULN 
for at least one week or until the patient withdraws consent. 
Study drug administration will be permanently discontinued if any of the following occurs: 
• ALT or AST > 8X ULN; 
• ALT or AST > 5X ULN for more than 2 weeks; 
• ALT or AST > 3X ULN and (TBL > 2X ULN or INR > 1.5); 

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• ALT or AST > 3X ULN with the appearance of fatigue, nausea, vomiting, right upper 
quadrant pain or tenderness, fever, rash, and/or eosinophilia (> 5%).  
The hepatobiliary tree must be visualized (e.g., ultrasound, magnetic resonance imaging) and 
assessed if a patient discontinues taking study drug secondary to elevated transaminase levels.  
Additional tests/studies may be warranted depending on the clinical presentation. 
9.1.3. 
Management of Muscle Spasms 
Basic symptomatic relief is the first step in managing muscle spasm, including walking, adequate 
hydration, wearing socks, and stretching before bedtime.  Assessment of levels of electrolytes 
such as magnesium, calcium and potassium as part of patient’s standard of care may indicate the 
need for replacement.  If vitamin D levels are low, supplementation may be warranted.  Muscle 
relaxants may also help relieve symptoms. 
9.1.4. 
Weight Loss 
Ongoing assessments to ensure that the patient is receiving adequate nutrition and consideration 
of other etiologies of weight loss may be warranted for patients receiving bardoxolone methyl.   
9.1.5. 
Hypomagnesaemia 
In instances where a patient experiences hypomagnesaemia (evaluated as part of patient’s 
standard of care), defined as serum magnesium less than 1.3 mEq/L (0.65 mmol/L), 
consideration should be given to repletion of serum magnesium. 
9.1.6. 
Nausea 
Nausea may occur with higher doses of bardoxolone methyl.  Nausea AEs are typically mild and 
reversible within a few weeks after treatment initiation.  If symptoms will not resolve, dose 
de-escalation, with consultation of the medical monitor, may be necessary.   

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9.2. 
Description of Study Drug 
Bardoxolone methyl (RTA 402) drug product information is shown in Table 4.   
Table 4: 
Bardoxolone Methyl Drug Product Information 
Description 
Bardoxolone methyl capsule (5 mg, 
10 mg) 
Ingredients 
Bardoxolone methyl 
Methacrylic Acid – Ethyl Acrylate 
Copolymer 
Type A  
Silicified Microcrystalline Cellulose  
Hydroxypropyl Methylcellulose  
Lactose Monohydrate 
Sodium Lauryl Sulfate 
Colloidal Silicon Dioxide 
Magnesium Stearate  
Gelatin capsules 
Titanium Dioxide 
 
Route of Administration 
Oral 
9.3. 
Concomitant Medications 
9.3.1. 
Excluded Medications 
No other investigational drug or device should be taken as part of an interventional study during 
the conduct of this study. 
Patients who take investigational drug or device during the study should not discontinue study 
drug solely on this basis.  Consultation with the medical monitor should occur prior to study drug 
discontinuation or withdrawing a patient from the study. 
9.3.2. 
Permitted Medications 
Pulmonary arterial hypertension-specific background therapy should be taken as clinically 
indicated and prescribed by the physician for the duration of the study. 
Refer to Section 9.10.15 for guidelines on oxygen use during 6MWTs.  Initiation of 
supplemental long-term oxygen therapy (oxygen application for more than 30 consecutive days) 
or persistent modification of a pre-existing supplemental long-term oxygen therapy (i.e., need for 
a persistent increase of the amount of delivered oxygen for more than 30 days) due to worsening 
PAH is permitted.  
Diuretics may be prescribed as clinically indicated throughout the study.  Any changes to the 
doses of oxygen or diuretics throughout the course of the study must be recorded in the 
electronic case report form (eCRF).   

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9.4. 
Treatment Compliance 
The investigator or his/her designated and qualified representatives will only dispense study drug 
to patients enrolled in the study in accordance with the protocol.  The study drug must not be 
used for reasons other than that described in the protocol.  
9.5. 
Randomization 
Not applicable. 
9.6. 
Blinding 
Not applicable.  
9.7. 
Unscheduled Visits 
Unscheduled visits are allowed for the following reasons: 
• Assessment of weight gain per Section 9.1.1;  
• Management of an AE or SAE; 
• Performance of additional laboratory tests for clinically abnormal laboratory test 
values or to confirm a possible pregnancy; 
• Dose de-escalation; 
• Any time the investigator feels that it is clinically appropriate for patient safety. 
At a minimum, unscheduled visits should include collection of AEs, concomitant medications 
and vital signs.  Additional conversations may be necessary with the medical monitor following 
an unscheduled visit to assess patient safety. 
9.8. 
Pregnancy 
9.8.1. 
Women of Childbearing Potential 
Women of childbearing potential (WOCBP) are those who are not surgically sterile (no history 
of bilateral tubal ligation, hysterectomy, or bilateral salpingo-oophorectomy), do not have 
fallopian inserts with confirmed blockage, have not had reproductive potential terminated by 
radiation, and are not postmenopausal for at least 1 year. 
9.8.2. 
Methods of Birth Control 
While taking study drug and until 30 days following administration of the final dose of study 
medication, WOCBP must practice one of the following acceptable methods of birth control: 
• Use of hormonal contraceptives (oral, parenteral, vaginal, or transdermal) for at least 
90 days prior to start of study drug administration; 
• Use of an intrauterine device; 

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• Abstain from sexual intercourse completely.  Complete abstinence from sexual 
intercourse is only acceptable if it is the preferred and usual lifestyle of the individual.  
Periodic abstinence is not permitted. 
While taking study drug and until 30 days after the final dose of study medication is taken, males 
who have female partners of childbearing potential must practice one of the following methods 
of birth control: 
• Have had a vasectomy (at least 6 months earlier); 
• Partner use of an intrauterine device; 
• Partner use of hormonal contraceptives (oral, parenteral, vaginal or transdermal) for 
at least 90 days prior to start of study drug administration; 
• Abstain from sexual intercourse completely.  Complete abstinence from sexual 
intercourse is only acceptable if it is the preferred and usual lifestyle of the individual.  
Periodic abstinence is not permitted. 
9.8.3. 
Suspected Pregnancy 
During the study, all WOCBP must be instructed to contact the investigator immediately if they 
suspect they might be pregnant (e.g., late or missed menstrual period).  Male patients must be 
instructed to contact the investigator if a sexual partner suspects she may be pregnant. 
If a patient or investigator suspects that the patient may be pregnant, the study drug must be 
withheld until the results of a serum pregnancy test are available.  If the serum pregnancy test 
confirms the pregnancy, the patient must permanently discontinue taking study drug.  The 
investigator must immediately report to the medical monitor a pregnancy associated with study 
drug exposure.  The early discontinuation protocol-required procedures outlined for End-of-
Study visits must be performed on the patient.  
Pregnancy is not considered an AE; however, the investigator must follow a pregnant patient, or 
the pregnant female partner of a male patient (if consenting), and report follow-up information 
regarding the course of the pregnancy, including perinatal and neonatal outcome.  Infants 
resulting from such pregnancies should be followed for a minimum of 8 weeks.  Reata or 
designee may contact the investigator to request additional information throughout the course of 
the pregnancy. 
The following pregnancy outcomes must be considered SAEs and will require additional 
reporting in the eCRF and reported as an SAE: 
• Congenital anomaly/birth defect; 
• Stillbirth; 
• Spontaneous miscarriage. 
9.9. 
Serious Toxicities 
In the case of serious toxicities, the investigator may choose to interrupt treatment with 
bardoxolone methyl.  A single dose reduction (from 10 mg to 5 mg) is permitted if clinically 
indicated to manage tolerability issues.  Once a patient’s dose has been reduced, that dose must 

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be maintained.  Patients who resume therapy after an interruption will follow the originally 
planned study schedule.  
9.10. 
Study Procedures  
The following sections describe each assessment.  The timing of these assessments is noted in 
Table 3.  All Day 1 procedures, except AE assessments, should be completed prior to 
administration of first dose of study drug. 
9.10.1. 
Informed Consent 
Written informed consent (see Section 15.3) must be obtained from the patient before any 
study-related procedures are performed, and again if there is a change in the study procedures 
that would affect the patient’s willingness to participate. 
9.10.2. 
Inclusion/Exclusion 
Inclusion and exclusion criteria must be reviewed as indicated in Table 3.  Patients must meet all 
of the inclusion and none of the exclusion criteria for entry in the study.   
9.10.3. 
Prior and Current Concomitant Medications 
The name, dose, and frequency must be recorded for all medications that the patient is taking.  
All allowed and excluded medications should be recorded including all prescription drugs, herbal 
products, vitamins, minerals, and over-the-counter medications.  Trade or generic drug names 
should be used where possible.  Concomitant medications will be reviewed as indicated in  
Table 3 and all changes will be recorded. 
9.10.4. 
Current PAH Background Medications 
The name, dose, and frequency must be recorded for all PAH background medications that the 
patient is taking.  PAH background medications are those that have been approved by the US 
Food and Drug Administration (FDA) or the local health authority for the treatment of PAH.  
Trade or generic drug names should be used where possible.  PAH background medications will 
be reviewed as indicated in Table 3 and all changes will be recorded.   
9.10.5. 
Weight and Body Mass Index  
Weight must be measured as indicated in Table 3.  Body mass index (BMI) will be calculated in 
the eCRF each time the weight is recorded.  Patients will use Sponsor-provided scales to measure 
weight at home.  Patients will be instructed to stop administering study drug and contact the 
investigator if their daily weight increases per the criteria outlined in Section 9.1.1.  Patients will 
be provided instructions within the Informed Consent Form to help ensure consistent weight 
collection throughout the study. 
9.10.6. 
Vital Sign Measurements 
Vital sign measurements include the patient’s heart rate (beats/minute taken for at least 
15 seconds), respiration rate, and body temperature.  Blood pressure should be taken after the 
patient has rested in a sitting position for at least 5 minutes.  The same arm (usually the non-

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dominant arm) and the appropriate size cuff should be used for each measurement.  Vital sign 
measurements should be taken as indicated in Table 3.  
9.10.7. 
Physical Examination 
A comprehensive physical examination must be performed by a physician, physician assistant, or 
registered nurse practitioner as indicated in Table 3 and as documented within the table 
footnotes.  The examination must include the following organ or body system assessments: head, 
eyes, ears, nose, throat, musculoskeletal, cardiovascular, lymphatic, respiratory, abdomen, skin, 
extremities, and neurological.  Assessments of any specific signs or symptoms reported by the 
patient must also be performed and documented along with any other findings of note.  Clinically 
significant findings at Day 1 must be recorded as medical history.  Following the examination on 
Day 1, new or changed physical examination findings meeting the criteria for an adverse event 
must be recorded as an adverse event.  If possible, the same individual should perform each 
physical examination on a patient during the study. 
9.10.8. 
Pregnancy Test 
WOCBP (see Section 9.8) will complete a pregnancy test as indicated in Table 3, or at any time 
if pregnancy is suspected.  Negative test results are required on Day 1 before study drug 
administration.  Any patient who becomes pregnant during the study must discontinue taking 
study drug immediately.  See Section 9.8.3 for a description of procedures to be followed in case 
of pregnancy. 
9.10.9. 
Study Drug Administration 
Patients should self-administer one capsule orally, once a day, preferably in the morning, as 
indicated in Table 3.  A vomited dose must not be replaced.  A double dose (e.g., missed dose 
from previous day and dose for current day) must not be taken. 
9.10.10. 
Study Drug Dispensation and Collection 
Study drug will be dispensed to the patient and collected from the patient as indicated in Table 3.  
The patient will be dispensed 1 or more treatment kits at Day 1, Week 4, Week 24, and every 
24 weeks thereafter for the duration of the study.  Dispensed treatment kits from each visit 
should be returned to the site for collection at the subsequent visit. 
9.10.11. 
Telephone Contact 
Patients will be contacted by telephone as indicated in Table 3.  Patients will be asked about their 
body weight and other signs of fluid retention, as well as AEs and any changes to concomitant 
medications.  If fluid retention is suspected, the patient must be brought into the clinic and 
evaluated by the investigator as soon as possible, as detailed in Section 9.1.1. 
9.10.12. 
Adverse Event Collection 
Patients will be observed for general appearance, presence of illness or injury, or signs indicative 
of a concurrent illness as indicated in Table 3.  Patients must be instructed to volunteer any 
information regarding AEs on or after the first dose of study drug or query the patients with an 
open question regarding any AEs they may be experiencing (e.g., “How have you been feeling 

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since your last visit?”).  Any findings are to be documented.  Patients must be asked if they have 
been hospitalized, had any accidents, used any new medications, or changed concomitant 
medication regimens (including prescription drugs, over-the-counter medications, vitamins, 
herbal products, and minerals).  Responses must be documented in the source documents. 
9.10.13. 
Clinical Chemistry 
Samples will be collected for clinical chemistry analyses as indicated in Table 3.  Clinical 
chemistries will be analyzed locally and should include those routinely monitored per patient’s 
standard of care.  
9.10.14. 
N-Terminal Pro-Brain Natriuretic Peptide and Brain Natriuretic Peptide  
Samples will be collected for NT-Pro BNP and/or BNP as indicated in Table 3.  As recent 
exercise may affect BNP and NT-Pro BNP levels, patients must be allowed to rest for a 
minimum period of 1 hour following arrival at the clinic and prior to obtaining this blood 
sample.  Similarly, this sample must be taken prior to the 6MWT or at least one hour after 
6MWT.  This sample must be taken with the patient in the same position at all appropriate visits, 
e.g., sitting or semi-recumbent. 
9.10.15. 
6-Minute Walk Test 
A 6-minute walk test (6MWT) will be performed by the patients as indicated in Table 3.  Unless 
otherwise indicated as described below, each test must be performed in strict accordance with the 
American Thoracic Society (ATS) guidelines (ATS Statement 2002) provided in Section 19, 
Appendix 1.  The appropriate language version will be applied for patients with a non-English 
informed consent.  The walking course must be 30 m in length, except at sites granted prior 
Sponsor agreement. 
Patients will rate their dyspnea and overall fatigue using the Borg scale (2004 version, provided 
in Section 21, Appendix 3) prior to beginning and immediately following completion of each 
6MWT as indicated in Table 3.   
The assessor of the 6MWT must not provide the patient with the results of the assessment once 
complete.  A patient should wear the same or similar shoes for all 6MWTs.  Additionally, if a 
patient uses a walking aid for the first test, this same aide should be used during all subsequent 
tests.  The shoes worn and walking aid used for each 6MWT should be noted in source 
documents.   
Patients who require supplemental oxygen with exercise may use their prescribed oxygen level 
during 6MWTs.  If a patient’s health status deteriorates during study participation and it is 
determined by a physician that the patient requires additional supplemental oxygen, the patient 
may continue study participation.   
9.10.16. 
WHO/NYHA Functional Class Assessment 
WHO/NYHA functional class will be assessed (Section 20, Appendix 2) as indicated in Table 3.  
To the extent feasible, the same evaluator should assess WHO/NYHA FC for a particular patient 
over the entire course of the study.  

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10. 
STUDY DRUG MATERIALS AND MANAGEMENT 
10.1. 
Study Drug 
Bardoxolone methyl capsules, 5 mg and 10 mg, will be used in this study. 
10.2. 
Study Drug Packaging and Labeling 
The study drug will be supplied in tamper-evident kits containing one 30-cc (5-mg capsules, 
30 count), one 60-cc (5-mg capsules, 90 count), or one 100-cc (10-mg capsules, 90 count) high-
density polyethylene bottle.  Each bottle will utilize foil induction-seal liners and a child-resistant 
closure.  Each bottle of study drug will contain 30 or 90 capsules of 5 mg or 10 mg strength 
bardoxolone methyl.  Each bottle will also contain a desiccant insert that must not be ingested.  
Labeling on each kit bottle will contain at minimum the following information: 
• Medication identification number; 
• Protocol 402-C-1602; 
• Caution Statement: New Drug – Limited by Federal Law to Investigational Use.  
Keep out of sight and reach of children; 
• Control or lot number 
• Store at 20° - 25°C (68° - 77°F), short term excursions allowed to 
15° - 30°C (59° - 86°F); 
• Reata Pharmaceuticals, Inc., Irving, TX. 
A double panel label will be presented on the treatment bottle containing this and other 
information as well.  Additionally, labeling in the relevant local languages for investigational 
medicinal product for use and distribution in the EU shall adhere to current Eudralex, Volume 4 
Annex 13 guidance and requirements.   
10.3. 
Study Drug Storage 
The stability of the drug product has been and is currently being evaluated in ongoing studies. 
Investigative sites must store the investigational product in a secure location with room 
temperature conditions of 20°C to 25°C (68°F to 77°F), with brief excursions allowed to 
15°C to 30°C (59°F to 86°F).   
10.4. 
Study Drug Administration 
Please refer to Section 9.10.9 for details on study drug administration.  Clear instructions will be 
provided to the patient regarding the number and type of capsules to be ingested at each study 
drug administration time point listed in Table 3.  Patients must be instructed to continue taking 
study drug unless: (1) patient has been otherwise instructed by the investigator or (2) the patient 
has been formally discontinued from the study.  

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10.5. 
Study Drug Accountability 
The investigator, or designee, will maintain a record of all study drug received, dispensed, and 
returned to the Sponsors’ designee.  No study drug shall be destroyed by the clinical site unless 
directed in writing to do so by the Sponsor’s quality assurance department.  Study drug bottles 
and any unused capsules should be returned to the study staff for eventual disposition by the 
Sponsor.  The number of capsules returned at each visit will be recorded for each bottle in the 
kit. 
10.6. 
Study Drug Handling and Disposal 
At the conclusion of the study or in an instance of planned study drug replacement, the Sponsor 
or its designee will direct the site regarding the final disposition of study drug. 

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11. 
SAFETY ASSESSMENTS 
11.1. 
Safety Parameters 
To avoid inter-observer variability, every effort should be made to ensure that the same 
individual who made the initial baseline determinations will complete all safety assessments.  
Safety parameters will include vital sign measurements, physical examination results, AEs, 
SAEs, weight, and assessment of BNP and/or NT-Pro BNP.  
11.2. 
Adverse and Serious Adverse Events 
11.2.1. 
Definition of Adverse Events 
11.2.1.1. Adverse Event 
An AE is defined as any untoward medical occurrence in a patient regardless of its causal 
relationship to study drug.  An AE can be any unfavorable and unintended sign (including any 
clinically significant abnormal laboratory test result), symptom, or disease temporally associated 
with the use of the study drug, whether or not it is considered to be study-drug related.  Included 
in this definition are any newly-occurring events or previous condition that has increased in 
severity or frequency since the administration of study drug. 
All AEs that are observed or reported by the patient during the study (from time of 
administration of the first dose at the Day 1 visit until the final visit indicated in Table 3) must be 
reported, regardless of their relationship to study drug or their clinical significance. 
11.2.1.2. Serious Adverse Event 
An SAE is any AE occurring at any dose and regardless of causality that: 
• Results in death; 
• Is life-threatening; 
• Requires inpatient hospitalization or prolongation of existing hospitalization; 
• Results in persistent or significant incapacity or substantial disruption of the ability to 
conduct normal life functions; 
• Is a congenital anomaly or birth defect in an offspring of a patient taking study drug; 
• Is an important medical event.  
The term "life-threatening" refers to an event in which the patient was at immediate risk of death 
at the time of the event.  It does not refer to an event that hypothetically might have caused death 
if it were more severe. 
Important medical events are those that may not meet any of the criteria defined above; however, 
they may be considered serious when, based upon appropriate medical judgment, they may 
jeopardize the patient and may require medical or surgical intervention to prevent one of the 
other outcomes listed in the SAE definition. 

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Pregnancy is not considered an AE; however, information will be collected for any pregnancies 
that occur during the study (from the time of the first dose of study drug until the final visit 
indicated in Table 3, as appropriate).  Certain pregnancy outcomes will require submission as an 
SAE (See Section 9.8). 
The investigator is responsible for reporting to Reata or designee all AEs and SAEs that are 
observed or reported by the patient during the study (from the time of administration of the first 
dose of study drug until the final visit indicated in Table 3, as appropriate), regardless of their 
relationship to study drug or their clinical significance.   
The Sponsor, or the Contract Research Organization (CRO) on the behalf of the Sponsor, must 
be notified immediately regarding the occurrence of any SAE that occurs after the patient 
receives the first dose of study drug and throughout the study, regardless of study drug 
administration, including SAEs resulting from protocol-associated procedures, as defined in 
relevant legislation.  The procedures for reporting all SAEs, regardless of causal relationship, are 
as follows: 
• Record the SAE on the AE eCRF and complete the “Serious Adverse Event Report” 
form within the electronic database; 
• In the event the electronic database is not functional, a paper SAE form will be 
available for the reporting of SAEs. 
The Sponsor may request additional information from the investigator to ensure the timely 
completion of accurate safety reports. 
All SAEs reported or observed during the study must be followed to resolution or until the 
investigator deems the event to be chronic or the patient to be stable.  Reata or designee may 
contact the investigator to obtain additional information on any SAE which has not resolved at 
the time the patient completes the study. 
11.3. 
Eliciting Adverse Event Information  
At every study visit, patients must be asked a standard, nondirected question, such as, “How 
have you been feeling since your last visit?” to elicit any medically related changes in their 
well-being.  They may also be asked if they have been hospitalized, had any accidents, used any 
new medications, or changed concomitant medication regimens (including prescription drugs, 
over-the-counter medications, vitamins, herbal products, and minerals).  Responses must be 
documented in the source documents. 
In addition to patient observations, AEs must be documented for any clinically significant 
diagnosis resulting from abnormal laboratory test values that are monitored per patient’s standard 
of care, physical examination findings, or from other documents that are relevant to patient 
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11.4. 
Assessment of Causality  
The investigator must use the following classifications and criteria to characterize the 
relationship or association of the study drug in causing or contributing to the AE: 
Not Related: This relationship suggests that there is no association between the study drug and 
the reported event. 
Unlikely Related: This relationship suggests that the temporal sequence of the event with study 
drug administration makes a causal relationship improbable and/or other factors also provide 
plausible explanations. 
Possibly Related: This relationship suggests that treatment with the study drug caused or 
contributed to the AE.  That is, the event follows a reasonable temporal sequence from the time 
of study drug administration, and/or, follows a known response pattern to the study drug, but 
could have been produced by other factors. 
Probably Related: This relationship suggests that a reasonable temporal sequence of the event 
with study drug administration exists and, based upon the known pharmacological action of the 
drug, known or previously reported adverse reactions to the drug or class of drugs, or judgment 
based on the investigator’s clinical experience, the association of the event with study drug 
administration seems likely. 
Definitely Related: This relationship suggests that a definite causal relationship exists between 
the drug administration and the AE, and other conditions (e.g., concurrent illness, 
progression/expression of disease state, or concurrent medication reaction) do not appear to 
explain the event. 
11.5. 
Assessment of Severity 
The investigator will grade the severity of the AEs as mild, moderate, or severe using the 
following definitions: 
Mild: Symptoms causing no or minimal interference with usual social and functional activities; 
Moderate: Symptoms causing greater than minimal interference with usual social and functional 
activities; 
Severe: Symptoms causing inability to perform usual social and functional activities.  
11.6. 
Recording Adverse Events 
All conditions present prior to the administration of the first dose of study drug (Day 1) in a 
previous study with bardoxolone methyl should be documented as medical history in a previous 
study with bardoxolone methyl.  After the first dose, documentation of AEs shall continue until 
30 days following administration of the final dose of study medication under this protocol 
(i.e., through the End-of-Treatment visit), regardless of the relationship of the AE to study drug.  
Information to be collected will include type of event, date of onset, date of resolution, 
investigator-specified assessment of severity and relationship to study drug, seriousness, as well 
as any action taken.  

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While an AE is ongoing, changes in the severity (e.g., worsening and improving) should be 
noted in the source documents, but when documenting the AE, only the total duration and 
greatest severity should be recorded in the eCRF.  AEs characterized as intermittent will require 
documentation of onset and duration. 
All drug-related (possibly, probably, or definitely related, see Section 11.4) AEs and abnormal 
laboratory test results reported or observed during the study must be followed to resolution 
(either return to baseline or within normal limits).  All other AEs will be followed through the 
final visit indicated in Table 3, as appropriate. 
AEs resulting from concurrent illnesses, reactions to concurrent illnesses, reactions to concurrent 
medications, or progression of disease states must also be reported.  Preexisting conditions 
(present before the start of the AE collection period) will be considered concurrent medical 
conditions and should NOT be recorded as AEs.  However, if the patient experiences a 
worsening or complication of such a concurrent condition, the worsening or complication shall 
be recorded as an AE.  Investigators shall ensure that the AE term recorded captures the change 
in the condition (e.g., “worsening of…”).  Any improvement in condition shall be documented 
per Section 9.10.7. 
Each AE shall be recorded to represent a single diagnosis.  Accompanying signs (including 
abnormal laboratory test values) or symptoms shall NOT be recorded as additional AEs.  If a 
diagnosis is unknown, sign(s) or symptom(s) shall be recorded as an AE(s).  Changes in 
laboratory test values will only be considered AEs if they are judged to be clinically significant 
(i.e., if some action or intervention is required or if the investigator judges the change to be 
beyond the range of normal physiological fluctuation).  If abnormal laboratory test values will be 
the result of pathology for which there will be an overall diagnosis (e.g., increased creatinine 
levels in renal failure), only the diagnosis shall be reported as an AE. 
Elective procedures (surgeries or therapies) that will be scheduled prior to the start of AE 
collection will not be considered AEs.  These elective procedures shall not be recorded as AEs, 
but shall be documented in the patient’s source documents as elective (e.g., elective periodontal 
surgery).  However, if a pre-planned procedure will be performed early (e.g., as an emergency) 
because of a worsening of the preexisting condition, the worsening of the condition shall be 
captured as an AE. 
11.7. 
Reporting Serious Adverse Events 
Any AE the investigator will consider serious according to the previously described criteria must 
be reported within 24 hours from the time the site personnel first learn about the event.  
To report the SAE, the investigator will complete the SAE form in the clinical database and send 
to 
 (fax numbers listed in Table 5) within 24 hours of awareness.  If the clinical 
database is down for any reason, the manual SAE forms will be utilized; however sites are 
responsible for ensuring the information on the manual SAE forms is entered into the clinical 
database once available. 

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Table 5: 
SAE Reporting Contact Information 
 
 
 
  
Non-North American sites will use access codes 
specific to their country.  Contact information for 
non-North American sites for SAE reporting will 
be provided by the clinical team directly to the 
sites. 
For questions regarding SAE reporting, contact your study manager, monitor, or 
 
 
Follow-Up Reports 
The investigator must continue to follow the patient until the SAE has subsided or until the 
condition becomes chronic in nature, stabilizes (in the case of persistent impairment), or the 
patient dies. 
Within 24 hours of receipt of new information, the SAE form should be updated within the 
clinical database and submitted along with any supporting documentation (e.g., patient discharge 
summary or autopsy reports).  If the clinical database is down for any reason, the manual SAE 
forms should be used; however, sites are responsible for ensuring the updated information on the 
manual SAE forms is entered into the clinical database once available. 
The Sponsor or designee will notify regulatory agencies of any fatal or life-threatening 
unexpected events associated with the use of the study drug as soon as possible but no later than 
7 calendar days after the initial receipt of the information.  Initial notification will be followed by 
a written report within the timeframe established by the appropriate regulatory agency.  For other 
SAEs that do not meet the fatal or life-threatening unexpected criteria, but are reported to be 
associated with the use of the study drug, Reata or designee will notify the appropriate regulatory 
agencies in writing within the timeframe established by those regulatory agencies.  Reata or 
designee will provide copies of any reports to regulatory agencies regarding serious and 
unexpected SAEs to the investigators for review and submission to their IRB or EC, as 
appropriate.  
Principal investigators will be responsible for informing their IRB/EC of any SAEs at their site.  
SAE correspondence with regulatory authorities or IRBs/ECs must be submitted to the Sponsor 
or designee for recording in the study file. 
Note that the following AEs which are commonly observed in this patient population will not be 
reported to regulatory authorities as individual expedited reports, except in unusual 
circumstances: 
• Shortness of breath; 
• Lightheadedness/dizziness; 
• Syncope; 

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• Chest pain; 
• Palpitations; 
• Fatigue; 
• Edema/fluid retention; 
• Exertional dyspnea; 
• Hypoxemia. 
These events will be reviewed on a regular basis in aggregate and will be reported in an 
expedited manner if a safety signal is detected.  Regular safety study updates will be reported to 
regulatory authorities according to local guidelines. 

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12. 
STATISTICS 
12.1. 
Sample Size 
The aim of this long-term extended access study is primarily to provide continuing treatment to 
patients with bardoxolone methyl and to assess long-term safety and tolerability, hence no single 
primary variable has been identified.  Patient numbers will be determined by those who have 
been treatment-compliant and who have previously participated in clinical studies with 
bardoxolone methyl. 
12.2. 
Study Variables 
12.2.1. 
Safety Variables 
The safety variables will include results of physical examinations, laboratory test results (BNP 
and NT-Pro BNP), vital sign measurements, weight, AEs, and SAEs. 
12.3. 
Statistical Analyses 
A statistical analysis plan (SAP) detailing the analyses will be developed prior to the database 
lock.  All statistical analyses and data summaries will be performed using SAS® (Version 9.1 or 
higher) or other validated software.  The SAP will serve as the final arbiter of all statistical 
analyses.  Data will be summarized overall using descriptive statistics.  Continuous data will be 
summarized with number of patients (n), mean, median, minimum, maximum, relevant quartiles, 
standard deviation, coefficient of variation, and geometric mean (where applicable).  Categorical 
data will be summarized using frequency counts and percentages.  
12.3.1. 
Primary Analysis of Safety 
As the extension is of an open-label design with no comparator group, all statistical analyses will 
be descriptive.  Patients will be included in these analyses if they are included in the safety 
analysis and have both baseline and post-baseline data available for the variable being analyzed.  
The summary tables will be presented for the overall group of patients, and also split by previous 
treatment groups in prior bardoxolone methyl clinical studies.   
 

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13. 
DIRECT ACCESS TO SOURCE DATA/DOCUMENTS 
13.1. 
Study-Specific Committees  
No steering committee, data safety monitoring committee, adjudication committee, or clinical 
endpoint committee will be used in this study. 
13.2. 
Study Monitoring 
The study site will be monitored remotely by the CRO periodically during the study to ensure 
that all aspects of the protocol will be followed and will include an electronic data collection 
which has a set of automatic data checks with data queries for programmed data collection.  
There will be monitoring of study site by telephone to ensure that the drug supplies have been 
provided and protocol instructions are well understood and applied. 
The Sponsor or designee will monitor all aspects of the study for compliance with applicable 
government regulation with respect to the International Council for Harmonisation (ICH) 
guideline E6(R2): Good Clinical Practice: Consolidated Guideline and current standard operating 
procedures. 
13.3. 
Audits and Inspections 
Principal investigators and institutions involved in the study will permit study-related 
monitoring, audits, and IRB/EC review, and regulatory inspections, by providing direct access to 
all study records.  In the event of an audit, the principal investigator will agree to allow the 
Sponsor, representatives of the Sponsor, the FDA, and other relevant regulatory authorities 
access to all study records. 
The principal investigator shall promptly notify the Sponsor or designee of any audits scheduled 
by any regulatory authorities and shall promptly forward copies of any audit reports received to 
the Sponsor or designee. 

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14. 
QUALITY CONTROL AND QUALITY ASSURANCE 
14.1. 
Quality Assurance 
To ensure compliance with Good Clinical Practices and all applicable regulatory requirements, 
Reata may conduct a quality assurance audit of the investigator’s clinical site, including 
CTM/IMP storage facilities.   
14.2. 
Financial Disclosure  
Principal investigators and sub-investigators are required to provide financial disclosure 
information prior to starting the study.  In addition, the principal investigator and sub-
investigators must provide the Sponsor or designee with updated information, if any relevant 
changes occur during the course of the investigation and for one year following the completion 
of the study. 
No potential investigator who has a vested financial interest in the success of this study may 
participate in this study. 
14.3. 
Sponsor Obligations  
The Sponsor or designee is not financially responsible for further testing/treatment of any 
medical condition that may be detected during the Screening process.  In addition, in the absence 
of specific arrangements, the Sponsor or designee is not financially responsible for treatment of 
the patient’s underlying disease. 
14.4. 
Investigator Documentation 
Before beginning the study, the principal investigator will be asked to comply with ICH E6(R2) 
8.2 and Title 21 of the Code of Federal Regulations (CFR) by providing the essential documents 
to the Sponsor or designee, which include but are not limited to the following: 
• An original investigator-signed investigator agreement page of the protocol;  
• The IRB/EC approval of the protocol; 
• The IRB- or EC-approved informed consent, samples of site advertisements for 
recruitment for this study, and any other written information regarding this study that 
is to be provided to the patient or legal guardians; 
• A Form FDA 1572, fully executed, and all updates on a new fully executed Form 
FDA 1572; 
• Curricula vitae for the principal investigator and each sub-investigator listed on Form 
FDA 1572.  A curricula vitae and current licensure, as applicable, must be provided.  
The curricula vitae must have been signed and dated by the principal investigators 
and sub-investigators within 2 years before study start-up to indicate the documents 
are accurate and current; 

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• Completed financial disclosure forms (Section 14.2) to allow the Sponsor or designee 
to submit complete and accurate certification or disclosure statements required under 
US Title 21 CFR 54.  In addition, the investigators must provide to the Sponsor or 
designee a commitment to update this information promptly if any relevant changes 
occur during the course of the investigation and for 1 year following the completion 
of the study; 
• Laboratory certifications and normal ranges for any laboratories used by the site for 
the conduct of this study. 
14.5. 
Clinical Study Insurance 
In accordance with the respective national drug laws, the Sponsor has taken out patient liability 
insurance for all patients who give their consent and enroll in this study.  This insurance covers 
potential fatalities, physical injuries, or damage to health that may occur during the clinical 
study. 
14.6. 
Use of Information  
All information regarding bardoxolone methyl supplied by the Sponsor to the investigator is 
privileged and confidential.  The investigator agrees to use this information to accomplish the 
study and will not use it for other purposes without consent from the Sponsor.  Furthermore, the 
investigator is obligated to provide the Sponsor with complete data obtained during the study.  
The information obtained from the clinical study will be used towards the development of 
bardoxolone methyl and may be disclosed to regulatory authorities, other investigators, corporate 
partners, or consultants as required. 

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15. 
ETHICS 
15.1. 
Institutional Review Board or Ethics Committee Review 
The protocol and the proposed informed consent form must be reviewed and approved by a 
properly constituted IRB/EC before study start.  Each investigator must provide the Sponsor or 
its designee a signed and dated statement that the protocol and informed consent have been 
approved by the IRB/EC for that site before consenting patients.  Prior to study initiation, the 
investigator is required to sign a protocol signature page confirming agreement to conduct the 
study in accordance with this protocol and to give access to all relevant data and records to the 
Sponsor, its designee, and regulatory authorities as required. 
The IRB/EC chairperson or designee must sign all IRB/EC approvals and must identify the 
IRB/EC by name and address, the clinical protocol, and the date approval and/or favorable 
opinion was granted. 
The principal investigator is responsible for obtaining reviews of the clinical research at intervals 
specified by the IRB/EC, but not exceeding 1 year.  The principal investigator must supply the 
Sponsor or designee with written documentation of reviews of the clinical research. 
15.2. 
Ethical Conduct of the Study 
This clinical study was designed and shall be implemented and reported in accordance with the 
ICH Harmonized Tripartite Guidelines for Good Clinical Practice, with applicable local 
regulations (e.g., US Code of Federal Regulations Title 21, European Directive 2001/20/EC), 
and with the ethical principles laid down in the Declaration of Helsinki.  Continuous monitoring 
of the benefits and risks of bardoxolone methyl is performed by Reata in reviewing data from all 
ongoing and prior trials with bardoxolone methyl and by the safety monitoring committees that 
oversee safety for the ongoing placebo-controlled Phase 2 and Phase 3 trials (CATALYST, 
EudraCT 2016-000196-24; LARIAT, EudraCT 2016-004793-17).   
The principal investigator agrees to conduct the study in accordance with the International 
Conference on Harmonization (ICH) for Guidance for Industry on GCP ICH E6(R2) 
[https://www.ich.org/fileadmin/Public_Web_Site/ICH_Products/Guidelines/Efficacy/E6/E6_R2_
_Step_4_2016_1109.pdf] and the principles of the Declaration of Helsinki 
[https://www.wma.net/policies-post/wma-declaration-of-helsinki-ethical-principles-for-medical-
research-involving-human-subjects/].  The principal investigator must conduct all aspects of this 
study in accordance with all national, state, and local laws or regulations. 
15.3. 
Written Informed Consent 
Because the study will be conducted under a US Investigational New Drug Application, a signed 
informed consent form, in compliance with Title 21 of the US CFR Part 50, will be obtained 
from each patient before the patient enters the study.  For sites outside of the US, the signed 
consent will be obtained in accord with local regulations, ICH E6 (R2), and principles of the 
Declaration of Helsinki.  An informed consent template may be provided by the Sponsor or 
designee to the investigators.  The consent must be reviewed by the Sponsor or designee before 
IRB/EC submission.  Once reviewed, the consent will be submitted by the principal investigator 

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to his or her IRB/EC for review and approval before the start of the study.  If the informed 
consent form is revised during the course of the study, all participants affected by the revision 
must sign the revised IRB/EC-approved consent form. 
Before enrollment, each prospective patient will be given a full explanation of the study and be 
allowed to read the approved informed consent form.  Once the principal investigator or designee 
is assured that the patient understands the implications of participating in the study, the patient 
will be asked to give consent to participate in the study by signing the informed consent form.  
Eligible patients may only be included in the study after providing written (witnessed, where 
required by law or regulation), IRB/EC-approved informed consent.  Informed consent must be 
obtained before conducting any study-specific procedures (i.e., all of the procedures described in 
the protocol).  The process of obtaining informed consent must be documented in the patient 
source documents. 
Any changes to the proposed consent form suggested by the investigator must be agreed to by 
the Sponsor before submission to the IRB/EC, and a copy of the approved version and the notice 
of approval must be provided to the Sponsor’s designated monitor after IRB/EC approval. 
The principal investigator or designee will provide a copy of the informed consent form (signed 
copy to be provided per applicable law) to the patient and/or legal guardian.  The original form 
will be maintained in the patient’s medical records at the site. 
15.4. 
Confidentiality 
All laboratory specimens, evaluation forms, reports, and other records will be identified in a 
manner designed to maintain patient confidentiality.  All records will be kept in a secure storage 
area with limited access.  Clinical information will not be released without the written 
permission of the patient (or the patient’s guardian), except as necessary for monitoring and 
auditing by the Sponsor, its designee, the FDA or applicable regulatory authorities, or the 
IRB/EC. 
The principal investigator and all employees and coworkers involved with this study may not 
disclose or use for any purpose other than performance of the study, any data, record, or other 
unpublished confidential information disclosed to them for the purpose of the study.  Prior 
written agreement from the Sponsor or designee must be obtained for the disclosure of any said 
confidential information to other parties. 
15.5. 
Modification of the Protocol 
Any changes that arise after the approval of the protocol must be documented as protocol 
amendments.  The FDA or other applicable regulatory agencies must be notified of protocol 
amendments.  The changes will become effective only after approval of the Sponsor, the 
investigator, the IRB/EC, and where necessary, the applicable regulatory agency.  In cases when 
the protocol is modified to enhance patient safety, changes may be implemented and the 
amendment must be immediately submitted to the IRB/EC. 
The investigator is responsible for informing the IRB/EC of all problems involving risks to 
patients according to national legislation.  In case of urgent safety measures, the Sponsor will 

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immediately notify the investigators and relevant regulatory agencies, including FDA in accord 
with 21 CFR 312.32. 
15.6. 
Protocol Deviations 
The principal investigator or designee must document any protocol deviation.  The IRB/EC must 
be notified of all protocol deviations in a timely manner by the principal investigator or designee 
as appropriate.  Protocol deviations will be documented by the responsible monitor during 
monitoring visits, and those observations will be communicated to the investigator. 
If there is an immediate hazard to a patient the principal investigator may deviate from the 
protocol without prior Sponsor and IRB/EC approval.  The Sponsor and IRB/EC must be 
notified of the deviation. 

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16. 
DATA HANDLING AND RECORDKEEPING 
16.1. 
Retention of Records 
The investigator will maintain all study records according to ICH-GCP and applicable regulatory 
requirement(s).  Records will be retained for at least 2 years after the last marketing application 
submission or 2 years after formal discontinuation of the clinical development of the 
investigational product.  If the investigator withdraws from the responsibility of keeping the 
study records, custody must be transferred to a person willing to accept the responsibility.  The 
Sponsor must be notified in writing if a custodial change occurs.  
16.2. 
Case Report Forms 
All case report form data will be entered in paper or electronic forms at the investigational site.  
A 21 CFR Part 11 compliant Electronic Data Capture system will be used to capture data 
electronically for all enrolled patients. 

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17. 
PUBLICATION POLICY 
The Sponsor supports communication and publication of study results whatever the findings of 
the study.   
The Sponsor reserves the right to review all planned communications and manuscripts based on 
the results of this study.  This reservation of the right is not intended to restrict or hinder 
publication or any other dissemination of study results, but to allow the Sponsor to confirm the 
accuracy of the data, to protect proprietary information, and to provide comments based on 
information that may not yet be available to the study investigators.  The Sponsor also 
encourages disclosure of any conflict of interest from all authors or investigators when 
manuscripts are submitted for publication.  Those individuals, who have contributed greatly to 
this study, including lead external advisors and select principal investigators, may serve on any 
potential publications committee for the study. 

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18. 
REFERENCES 
ATS Committee on Proficiency Standards for Clinical Pulmonary Function Laboratories. ATS 
Statement: Guidelines for the six-minute walk test.  Am J Respir Crit Care Med. 
2002;166(1):111-7. 
Batt J, Ahmed SS, Correa J, et al. Skeletal muscle dysfunction in idiopathic pulmonary arterial 
hypertension. Am J Respir Cell Mol Biol. 2014;50(1):74-86. 
Chung L, Domsic RT, Lingala B, et al. Survival and predictors of mortality in systemic 
sclerosis-associated pulmonary arterial hypertension: outcomes from the pulmonary hypertension 
assessment and recognition of outcomes in scleroderma registry. Arthritis Care Res (Hoboken). 
2014;66(3):489-95. 
Dinkova-Kostova AT, Liby KT, Stephenson KK, et al. Extremely potent triterpenoid inducers of 
the phase 2 response: correlations of protection against oxidant and inflammatory stress.  
Proc Natl Acad Sci U S A. 2005;102(12):4584-9. 
Eba S, Hoshikawa Y, Moriguchi T, et al. The nuclear factor erythroid 2-related factor 2 activator 
oltipraz attenuates chronic hypoxia-induced cardiopulmonary alterations in mice. Am J Respir 
Cell Mol Biol. 2013;49(2):324-33. 
Galiè N, Hoeper MM, Humbert M, et al. Guidelines for the diagnosis and treatment of 
pulmonary hypertension: the Task Force for the Diagnosis and Treatment of Pulmonary 
Hypertension of the European Society of Cardiology (ESC) and the European Respiratory 
Society (ERS), endorsed by the International Society of Heart and Lung Transplantation 
(ISHLT). Eur Heart J. 2009;30:2493-537. 
Ghofrani HA, Galiè N, Grimminger F, et al. Riociguat for the treatment of pulmonary arterial 
hypertension. N Engl J Med. 2013;369(4):330-40. 
Holmström KM, Baird L, Zhang Y, et al. Nrf2 impacts cellular bioenergetics by controlling 
substrate availability for mitochondrial respiration. Biol Open. 2013;0:1-10. 
Lee DF, Kuo HP, Liu M, et al. KEAP1 E3 ligase-mediated downregulation of NF-kB signaling 
by targeting IKKbeta. Mol Cell. 2009;36(1):131-40. 
Ludtmann MH, Angelova PR, Zhang Y, et al. Nrf2 impacts the efficiency of mitochondrial fatty 
acid oxidation. Biochem J. 2014;457(3):415-24. 
Mainguy V, Maltais F, Saey D, et al. Peripheral muscle dysfunction in idiopathic pulmonary 
hypertension. Thorax. 2010;65(2):113-7. 
Malenfant S, Potus F, Fournier F, et al. Skeletal muscle proteomic signature and metabolic 
impairment in pulmonary hypertension. J Mol Med (Berl). 2015;93(5):573-84. 
Osburn WOand, Kensler TW. Nrf2 signaling: An adaptive response pathway for protection 
against environmental toxic insults. Mutat Res. 2008;659(1-2):31-9. 
Price L, Caramori G, Dorfmuller P, et al.  Endothelial cell NF-kB activation is increased in 
human idiopathic PAH. Thorax. 2010;65(4):A88-A89. 

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Pugh ME; Robbins, IM; Rice TW, et al. Unrecognized glucose intolerance is common in 
pulmonary arterial hypertension. J Heart Lung Transplant. 2011;30(8):904-11. 
Rojas-Rivera J, Ortiz A, Egido J. Antioxidants in kidney diseases: The impact of bardoxolone 
methyl. Int J Nephrol. 2012;2012:1-11. 
Saha PK, Reddy VT, Konopleva M, et al. The triterpenoid 2-cyano-3,12-dioxooleana-1,9-dien-
28-oic-acid methyl ester has potent antidiabetic effects in diet-induced diabetic mice and 
Lepr(db/db) mice. J Biol Chem. 2010;285(52):40581-92. 
Sutendra G, Michelakis ED. The metabolic basis of pulmonary arterial hypertension. Cell Metab. 
2014;19(4):558-73. 
Tapson VF, Jing ZC, Xu KF, et al. Oral treprostinil for the treatment of pulmonary arterial 
hypertension in patients receiving background endothelin receptor antagonist and 
phosphodiesterase type 5 inhibitor therapy (the FREEDOM-C2 study): a randomized controlled 
trial. Chest. 2013;144(3):952-8. 
Wu QQ, Wang Y, Senitko M, et al. Bardoxolone methyl (BARD) ameliorates ischemic AKI and 
increases expression of protective genes Nrf2, PPARγ, and HO-1. Am J Physiol Renal Physiol. 
2011;300(5):F1180-F1192. 
 

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19. 
APPENDIX 1: ATS GUIDELINES FOR THE SIX-MINUTE WALK 
TEST 
 

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American Thoracic Society
Am J Respir Crit Care Med
Vol 166. pp 111–117, 2002
DOI: 10.1164/rccm.166/1/111
Internet address: www.atsjournals.org
ATS Statement: Guidelines for the Six-Minute Walk Test
THIS OFFICIAL STATEMENT OF THE AMERICAN THORACIC SOCIETY WAS APPROVED BY THE ATS BOARD OF DIRECTORS
MARCH 2002
CONTENTS
Purpose and Scope
Background
Indications and Limitations
Contraindications
Safety Issues
Technical Aspects of the 6-Minute Walk Test
Required Equipment
Patient Preparation
Measurements
Quality Assurance
Interpretation
References
PURPOSE AND SCOPE
This statement provides practical guidelines for the 6-minute
walk test (6MWT). Specifically, it reviews indications, details
factors that influence results, presents a brief step-by-step pro-
tocol, outlines safety measures, describes proper patient prep-
aration and procedures, and offers guidelines for clinical inter-
pretation of results. These recommendations are not intended
to limit the use of alternative protocols for research studies.
We do not discuss the general topic of clinical exercise testing.
As with other American Thoracic Society statements on
pulmonary function testing, these guidelines come out of a
consensus conference. Drafts were prepared by two members
(P.L.E. and R.J.Z.) and were based on a comprehensive Med-
line literature search from 1970 through 2001, augmented by
suggestions from other committee members. Each draft re-
sponded to comments from the working committee. The guide-
lines follow previously published methods as closely as possi-
ble and provide a rationale for each specific recommendation.
The final recommendations represent a consensus of the com-
mittee. The committee recommends that these guidelines be
reviewed in five years and in the meantime encourages further
research in areas of controversy.
BACKGROUND
There are several modalities available for the objective evalu-
ation of functional exercise capacity. Some provide a very
complete assessment of all systems involved in exercise per-
formance (high tech), whereas others provide basic informa-
tion but are low tech and are simpler to perform. The modality
used should be chosen based on the clinical question to be ad-
dressed and on available resources. The most popular clinical
exercise tests in order of increasing complexity are stair climb-
ing, a 6MWT, a shuttle-walk test, detection of exercise-induced
asthma, a cardiac stress test (e.g., Bruce protocol), and a cardio-
pulmonary exercise test (1, 2). Other professional organiza-
tions have published standards for cardiac stress testing (3, 4).
Assessment of functional capacity has traditionally been
done by merely asking patients the following: “How many
flights of stairs can you climb or how many blocks can you
walk?” However, patients vary in their recollection and may
report overestimations or underestimations of their true func-
tional capacity. Objective measurements are usually better
than self-reports. In the early 1960s, Balke developed a simple
test to evaluate the functional capacity by measuring the dis-
tance walked during a defined period of time (5). A 12-minute
field performance test was then developed to evaluate the
level of physical fitness of healthy individuals (6). The walking
test was also adapted to assess disability in patients with
chronic bronchitis (7). In an attempt to accommodate patients
with respiratory disease for whom walking 12 minutes was too
exhausting, a 6-minute walk was found to perform as well as
the 12-minute walk (8). A recent review of functional walking
tests concluded that “the 6MWT is easy to administer, better
tolerated, and more reflective of activities of daily living than
the other walk tests” (9).
The 6MWT is a practical simple test that requires a 100-ft
hallway but no exercise equipment or advanced training for
technicians. Walking is an activity performed daily by all but
the most severely impaired patients. This test measures the dis-
tance that a patient can quickly walk on a flat, hard surface in a
period of 6 minutes (the 6MWD). It evaluates the global and in-
tegrated responses of all the systems involved during exercise,
including the pulmonary and cardiovascular systems, systemic
circulation, peripheral circulation, blood, neuromuscular units,
and muscle metabolism. It does not provide specific informa-
tion on the function of each of the different organs and systems
involved in exercise or the mechanism of exercise limitation, as
is possible with maximal cardiopulmonary exercise testing. The
self-paced 6MWT assesses the submaximal level of functional
capacity. Most patients do not achieve maximal exercise capac-
ity during the 6MWT; instead, they choose their own intensity
of exercise and are allowed to stop and rest during the test.
However, because most activities of daily living are performed
at submaximal levels of exertion, the 6MWD may better reflect
the functional exercise level for daily physical activities.
INDICATIONS AND LIMITATIONS
The strongest indication for the 6MWT is for measuring the re-
sponse to medical interventions in patients with moderate to
severe heart or lung disease. The 6MWT has also been used as
a one-time measure of functional status of patients, as well as a
predictor of morbidity and mortality (see Table 1 for a list of
these indications). The fact that investigators have used the
6MWT in these settings does not prove that the test is clinically
useful (or the best test) for determining functional capacity or
changes in functional capacity due to an intervention in pa-
tients with these diseases. Further studies are necessary to de-
termine the utility of the 6MWT in various clinical situations.

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dial infarction during the previous month. Relative contraindi-
cations include a resting heart rate of more than 120, a systolic
blood pressure of more than 180 mm Hg, and a diastolic blood
pressure of more than 100 mm Hg.
Patients with any of these findings should be referred to the
physician ordering or supervising the test for individual clini-
cal assessment and a decision about the conduct of the test.
The results from a resting electrocardiogram done during the
previous 6 months should also be reviewed before testing. Sta-
ble exertional angina is not an absolute contraindication for a
6MWT, but patients with these symptoms should perform the
test after using their antiangina medication, and rescue nitrate
medication should be readily available.
Rationale
Patients with the previously mentioned risk factors may be at
increased risk for arrhythmias or cardiovascular collapse during
testing. However, each patient determines the intensity of their
exercise, and the test (without electrocardiogram monitoring)
has been performed in thousands of older persons (31, 48–50)
and thousands of patients with heart failure or cardiomyopathy
(32, 51, 52) without serious adverse events. The contraindica-
tions listed previously here were used by study investigators
based on their impressions of the general safety of the 6MWT
and their desire to be prudent, but it is unknown whether ad-
verse events would occur if such patients performed a 6MWT;
they are, therefore, listed as relative contraindications.
SAFETY ISSUES
1. Testing should be performed in a location where a rapid,
appropriate response to an emergency is possible. The ap-
propriate location of a crash cart should be determined by
the physician supervising the facility.
2. Supplies that must be available include oxygen, sublingual
nitroglycerine, aspirin, and albuterol (metered dose inhaler
or nebulizer). A telephone or other means should be in
place to enable a call for help.
3. The technician should be certified in cardiopulmonary re-
suscitation with a minimum of Basic Life Support by an
American Health Association–approved cardiopulmonary
resuscitation course. Advanced cardiac life support certifi-
cation is desirable. Training, experience, and certification
in related health care fields (registered nurse, registered re-
spiratory therapist, certified pulmonary function techni-
cian, etc.) are also desirable. A certified individual should
be readily available to respond if needed.
4. Physicians are not required to be present during all tests.
The physician ordering the test or a supervising laboratory
physician may decide whether physician attendance at a
specific test is required.
5. If a patient is on chronic oxygen therapy, oxygen should be
given at their standard rate or as directed by a physician or
a protocol.
Reasons for immediately stopping a 6MWT include the follow-
ing: (1) chest pain, (2) intolerable dyspnea, (3) leg cramps, (4)
staggering, (5) diaphoresis, and (6) pale or ashen appearance.
Technicians must be trained to recognize these problems
and the appropriate responses. If a test is stopped for any of
these reasons, the patient should sit or lie supine as appropri-
ate depending on the severity or the event and the technician’s
assessment of the severity of the event and the risk of syncope.
The following should be obtained based on the judgment of
the technician: blood pressure, pulse rate, oxygen saturation,
and a physician evaluation. Oxygen should be administered as
appropriate.
Formal cardiopulmonary exercise testing provides a global
assessment of the exercise response, an objective determina-
tion of functional capacity and impairment, determination of
the appropriate intensity needed to perform prolonged exer-
cise, quantification of factors limiting exercise, and a defini-
tion of the underlying pathophysiologic mechanisms such as
the contribution of different organ systems involved in exer-
cise. The 6MWT does not determine peak oxygen uptake, di-
agnose the cause of dyspnea on exertion, or evaluate the
causes or mechanisms of exercise limitation (1, 2). The infor-
mation provided by a 6MWT should be considered comple-
mentary to cardiopulmonary exercise testing, not a replace-
ment for it. Despite the difference between these two functional
tests, some good correlations between them have been re-
ported. For example, a significant correlation (r  0.73) be-
tween 6MWD and peak oxygen uptake has been reported for
patients with end-stage lung diseases (36, 37).
In some clinical situations, the 6MWT provides informa-
tion that may be a better index of the patient’s ability to per-
form daily activities than is peak oxygen uptake; for example,
6MWD correlates better with formal measures of quality of
life (38). Changes in 6MWD after therapeutic interventions
correlate with subjective improvement in dyspnea (39, 40).
The reproducibility of the 6MWD (with a coefficient of varia-
tion of approximately 8%) appears to be better than the re-
producibility of 1-second forced expiratory volume in patients
with chronic obstructive pulmonary disease (COPD) (8, 41–
43). Questionnaire indices of functional status have a larger
short-term variability (22–33%) than does the 6MWD (37).
The shuttle-walking test is similar to the 6MWT, but it uses
an audio signal from a tape cassette to direct the walking pace
of the patient back and forth on a 10-m course (44–47). The
walking speed is increased every minute, and the test ends when
the patient cannot reach the turnaround point within the re-
quired time. The exercise performed is similar to a symptom-
limited, maximal, incremental treadmill test. An advantage of
the shuttle walking test is that it has a better correlation with
peak oxygen uptake than the 6MWD. Disadvantages include
less validation, less widespread use, and more potential for
cardiovascular problems.
CONTRAINDICATIONS
Absolute contraindications for the 6MWT include the follow-
ing: unstable angina during the previous month and myocar-
TABLE 1. INDICATIONS FOR THE SIX-MINUTE WALK TEST
Pretreatment and posttreatment comparisons
Lung transplantation (9, 10)
Lung resection (11)
Lung volume reduction surgery (12, 13)
Pulmonary rehabilitation (14, 15)
COPD (16–18)
Pulmonary hypertension
Heart failure (19, 20)
Functional status (single measurement)
COPD (21, 22)
Cystic fibrosis (23, 24)
Heart failure (25–27)
Peripheral vascular disease (28, 29)
Fibromyalgia (30)
Older patients (31)
Predictor of morbidity and mortality
Heart failure (32, 33)
COPD (34, 35)
Primary pulmonary hypertension (10, 36)
Definition of abbreviation: COPD  chronic obstructive pulmonary disease.

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American Thoracic Society
TECHNICAL ASPECTS OF THE 6MWT
Location
The 6MWT should be performed indoors, along a long, flat,
straight, enclosed corridor with a hard surface that is seldom
traveled. If the weather is comfortable, the test may be per-
formed outdoors. The walking course must be 30 m in length.
A 100-ft hallway is, therefore, required. The length of the cor-
ridor should be marked every 3 m. The turnaround points should
be marked with a cone (such as an orange traffic cone). A start-
ing line, which marks the beginning and end of each 60-m lap,
should be marked on the floor using brightly colored tape.
Rationale.  A shorter corridor requires patients to take more
time to reverse directions more often, reducing the 6MWD.
Most studies have used a 30-m corridor, but some have used
20-
0- or 50-m corridors (52–55). A recent multicenter st
found no significant effect of the length of straight courses
ranging from 50 to 164 ft, but patients walked farther on con-
tinuous (oval) tracks (mean 92 ft farther) (54).
The use of a treadmill to determine the 6MWD might save
space and allow constant monitoring during the exercise, but
the use of a treadmill for 6-minute walk testing is not recom-
mended. Patients are unable to pace themselves on a tread-
mill. In one study of patients with severe lung disease, the
mean distance walked on the treadmill during 6 minutes (with
the speed adjusted by the patients) was shorter by a mean of
14% when compared with the standard 6MWD using a 100-ft
hallway (57). The range of differences was wide, with patients
walking between 400–1,300 ft on the treadmill who walked
1,200 ft in the hallway. Treadmill test results, therefore, are
not interchangeable with corridor tests.
REQUIRED EQUIPMENT
1. Countdown timer (or stopwatch)
2. Mechanical lap counter
3. Two small cones to mark the turnaround points
4. A chair that can be easily moved along the walking course
5. Worksheets on a clipboard
6. A source of oxygen
7. Sphygmomanometer
8. Telephone
9. Automated electronic defibrillator
PATIENT PREPARATION
1. Comfortable clothing should be worn.
2. Appropriate shoes for walking should be worn.
3. Patients should use their usual walking aids during the test
(cane, walker, etc.).
4. The patient’s usual medical regimen should be continued.
5. A light meal is acceptable before early morning or early af-
ternoon tests.
6. Patients should not have exercised vigorously within 2 hours
of beginning the test.
MEASUREMENTS
1. Repeat testing should be performed about the same time
of day to minimize intraday variability.
2.
3.
A “warm-up” period before the test should not be performed.
The patient should sit at rest in a chair, located near the
starting position, for at least 10 minutes before the test
starts. During this time, check for contraindications, mea-
sure pulse and blood pressure, and make sure that cloth-
ing and shoes are appropriate. Compete the first portion
of the worksheet (see the APPENDIX).
4. Pulse oximetry is optional. If it is performed, measure and
record baseline heart rate and oxygen saturation (SpO2)
and follow manufacturer’s instructions to maximize the sig-
nal and to minimize motion artifact (56, 57). Make sure the
readings are stable before recording. Note pulse regularity
and whether the oximeter signal quality is acceptable.
The rationale for measuring oxygen saturation is that al-
though the distance is the primary outcome measure, im-
provement during serial evaluations may be manifest either
by an increased distance or by reduced symptoms with the
same distance walked (39). The SpO2 should not be used for
constant monitoring during the exercise. The technician
must not walk with the patient to observe the SpO2. If worn
during the walk, the pulse oximeter must be lightweight (less
than 2 pounds), battery powered, and held in place (perhaps
by a “fanny pack”) so that the patient does not have to hold
or stabilize it and so that stride is not affected. Many pulse
oximeters have considerable motion artifact that prevents
accurate readings during the walk. (57)
5. Have the patient stand and rate their baseline dyspnea
and overall fatigue using the Borg scale (see Table 2 for
the Borg scale and instructions [58]).
6. Set the lap counter to zero and the timer to 6 minutes. As-
semble all necessary equipment (lap counter, timer, clip-
board, Borg Scale, worksheet) and move to the starting
point.
7. Instruct the patient as follows:
“The object of this test is to walk as far as possible for 6
minutes. You will walk back and forth in this hallway. Six
minutes is a long time to walk, so you will be exerting your-
self. You will probably get out of breath or become ex-
hausted. You are permitted to slow down, to stop, and to
rest as necessary. You may lean against the wall while rest-
ing, but resume walking as soon as you are able.
You will be walking back and forth around the cones.
You should pivot briskly around the cones and continue
back the other way without hesitation. Now I’m going to
show you. Please watch the way I turn without hesitation.”
Demonstrate by walking one lap yourself. Walk and
pivot around a cone briskly.
“Are you ready to do that? I am going to use this
counter to keep track of the number of laps you complete. I
will click it each time you turn around at this starting line.
Remember that the object is to walk AS FAR AS POSSI-
BLE for 6 minutes, but don’t run or jog.
Start now, or whenever you are ready.”
TABLE 2. THE BORG SCALE
0
Nothing at all
0.5
Very, very slight (just noticeable)
1
Very slight
2
Slight (light)
3
Moderate
4
Somewhat severe
5
Severe (heavy)
6
7
Very severe
8
9
10
Very, very severe (maximal)
This Borg scale should be printed on heavy paper (11 inches high and perhaps lami-
nated) in 20-point type size. At the beginning of the 6-minute exercise, show the scale
to the patient and ask the patient this: “Please grade your level of shortness of breath
using this scale.” Then ask this: “Please grade your level of fatigue using this scale.”
At the end of the exercise, remind the patient of the breathing number that they
chose before the exercise and ask the patient to grade their breathing level again. Then
ask the patient to grade their level of fatigue, after reminding them of their grade be-
fore the exercise.

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8. Position the patient at the starting line. You should also
stand near the starting line during the test. Do not walk
with the patient. As soon as the patient starts to walk,
start the timer.
9. Do not talk to anyone during the walk. Use an even tone
of voice when using the standard phrases of encourage-
ment. Watch the patient. Do not get distracted and lose
count of the laps. Each time the participant returns to the
starting line, click the lap counter once (or mark the lap
on the worksheet). Let the participant see you do it. Ex-
aggerate the click using body language, like using a stop-
watch at a race.
After the first minute, tell the patient the following (in
even tones): “You are doing well. You have 5 minutes to
go.”
When the timer shows 4 minutes remaining, tell the pa-
tient the following: “Keep up the good work. You have 4
minutes to go.”
When the timer shows 3 minutes remaining, tell the pa-
tient the following: “You are doing well. You are halfway
done.”
When the timer shows 2 minutes remaining, tell the pa-
tient the following: “Keep up the good work. You have only
2 minutes left.”
When the timer shows only 1 minute remaining, tell the
patient: “You are doing well. You have only 1 minute to
go.”
Do not use other words of encouragement (or body lan-
guage to speed up).
If the patient stops walking during the test and needs a
rest, say this: “You can lean against the wall if you would
like; then continue walking whenever you feel able.” Do
not stop the timer. If the patient stops before the 6 minutes
are up and refuses to continue (or you decide that they
should not continue), wheel the chair over for the patient to
sit on, discontinue the walk, and note on the worksheet the
distance, the time stopped, and the reason for stopping pre-
maturely.
When the timer is 15 seconds from completion, say this:
“In a moment I’m going to tell you to stop. When I do, just
stop right where you are and I will come to you.”
When the timer rings (or buzzes), say this: “Stop!” Walk
over to the patient. Consider taking the chair if they look
exhausted. Mark the spot where they stopped by placing a
bean bag or a piece of tape on the floor.
10.
11.
Post-test: Record the postwalk Borg dyspnea and fatigue
levels and ask this: “What, if anything, kept you from walk-
ing farther?”
If using a pulse oximeter, measure SpO2 and pulse rate
from the oximeter and then remove the sensor.
12. Record the number of laps from the counter (or tick marks
on the worksheet).
13. Record the additional distance covered (the number of meters
in the final partial lap) using the markers on the wall as dis-
tance guides. Calculate the total distance walked, rounding to
the nearest meter, and record it on the worksheet.
14. Congratulate the patient on good effort and offer a drink
of water.
QUALITY ASSURANCE
Sources of Variability
There are many sources of 6MWD variability (see Table 3).
The sources of variability caused by the test procedure itself
should be controlled as much as possible. This is done by fol-
lowing the standards found in this document and by using a
quality-assurance program.
Practice Tests
A practice test is not needed in most clinical settings but
should be considered. If a practice test is done, wait for at least
1 hour before the second test and report the highest 6MWD as
the patient’s 6MWD baseline.
Rationale.  The 6MWD is only slightly higher for a second
6MWT performed a day later. The mean reported increase
ranges from 0 to 17% (23, 27, 40, 41, 54, 59). A multicenter
study of 470 highly motivated patients with severe COPD per-
formed two 6MWTs 1 day apart, and on average, the 6MWD
was only 66 ft (5.8%) higher on the second day (54).
Performance (without an intervention) usually reaches a
plateau after two tests done within a week (8, 60). The training
effect may be due to improved coordination, finding optimal
stride length, and overcoming anxiety. The possibility of a
practice or training effect from tests repeated after more than
a month has not been studied or reported; however, it is likely
that the effect of training wears off (does not persist) after a
few weeks.
Technician Training and Experience
Technicians who perform 6MWTs should be trained using the
standard protocol and then supervised for several tests before
performing them alone. They should also have completed car-
diopulmonary resuscitation training.
Rationale.  One multicenter study of older people found
that after correction for many other factors, two of the techni-
cians had mean 6MWDs that were approximately 7% lower
than the other two sites (31).
Encouragement
Only the standardized phrases for encouragement (as speci-
fied previously here) must be used during the test.
Rationale.  Encouragement significantly increases the dis-
tance walked (42). Reproducibility for tests with and without
encouragement is similar. Some studies have used encourage-
ment every 30 seconds, every minute, or every 2 minutes. We
have chosen every minute and standard phrases. Some studies
(53)
53) have instructed patients to walk as fast as possible.
though larger mean 6MWDs may be obtained thereby, we rec-
ommend that such phrases not be used, as they emphasize ini-
tial speed at the expense of earlier fatigue and possible
excessive cardiac stress in some patients with heart disease.
TABLE 3. 6MWD SOURCES OF VARIABILITY
Factors reducing the 6MWD
Shorter height
Older age
Higher body weight
Female sex
Impaired cognition
A shorter corridor (more turns)
Pulmonary disease (COPD, asthma, cystic fibrosis, interstitial lung disease)
Cardiovascular disease (angina, MI, CHF, stroke, TIA, PVD, AAI)
Musculoskeletal disorders (arthritis, ankle, knee, or hip injuries, muscle wasting, etc.)
Factors increasing the 6MWD
Taller height (longer legs)
Male sex
High motivation
A patient who has previously performed the test
Medication for a disabling disease taken just before the test
Oxygen supplementation in patients with exercise-induced hypoxemia
Definition of abbreviations: COPD  chronic obstructive pulmonary disease; 6MWD  
6-minute walking distance.

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Supplemental Oxygen
If oxygen supplementation is needed during the walks and se-
rial tests are planned (after an intervention other than oxygen
therapy), then during all walks by that patient oxygen should
be delivered in the same way with the same flow. If the flow
must be increased during subsequent visits due to worsening
gas exchange, this should be noted on the worksheet and con-
sidered during interpretation of the change noted in 6MWD.
The type of oxygen delivery device should also be noted on
the report: for instance, the patient carried liquid oxygen or
pushed or pulled an oxygen tank, the delivery was pulsed or
continuous, or a technician walked behind the patient with the
oxygen source (not recommended). Measurements of pulse
and SpO2 should be made after waiting at least 10 minutes af-
ter any change in oxygen delivery.
Rationale.  For patients with COPD or interstitial lung dis-
ease, oxygen supplementation increases the 6MWD (17, 59,
61, 63). Carrying a portable gas container (but not using it for
supplemental oxygen) reduced the mean 6MWD by 14% in
one study of patients with severe respiratory disability, but us-
ing the container to deliver supplemental oxygen during the
exercise increased the mean 6MWD by 20–35% (59).
Medications
The type of medication, dose, and number of hours taken be-
fore the test should be noted.
Rationale.  Significant improvement in the distance walked,
or the dyspnea scale, after administration of bronchodilators
has been demonstrated in patients with COPD (62, 63), as well
as cardiovascular medications in patients with heart failure (19).
INTERPRETATION
Most 6MWTs will be done before and after intervention, and
the primary question to be answered after both tests have
been completed is whether the patient has experienced a clini-
cally significant improvement. With a good quality-assurance
program, with patients tested by the same technician, and af-
ter one or two practice tests, short-term reproducibility of the
6MWD is excellent (37). It is not known whether it is best for
clinical purposes to express change in 6MWD as (1) an abso-
lute value, (2) a percentage change, or (3) a change in the per-
centage of predicted value. Until further research is available,
we recommend that change in 6MWD be expressed as an ab-
solute value (e.g., the patient walked 50 m farther).
A statistically significant mean increase in 6MWD in a
group of study participants is often much less than a clinically
significant increase in an individual patient. In one study of
112 patients (half of them women) with stable, severe COPD,
the smallest difference in 6MWD that was associated with a
noticeable clinical difference in the patients’ perception of ex-
ercise performance was a mean of 54 m (95% confidence in-
terval, 37–71 m) (64). This study suggests that for individual
patients with COPD, an improvement of more than 70 m in
the 6MWD after an intervention is necessary to be 95% confi-
dent that the improvement was significant. In an observational
study of 45 older patients with heart failure, the smallest dif-
ference in 6MWD that was associated with a noticeable differ-
ence in their global rating of worsening was a mean of 43 m
(20). The 6MWD was more responsive to deterioration than
to improvement in heart failure symptoms.
Reported Mean Changes in 6MWD After Interventions
Supplemental oxygen (4 L/min) during exercise in patients with
COPD or interstitial lung disease increased mean 6MWD by
approximately 95 m (36%) in one study (59). Patients taking
an inhaled corticosteroid experienced a mean 33 m (8%) in-
crease in 6MWD in an international COPD study (16). Pa-
tients with COPD in a study of the effects of exercise and dia-
phragmatic strength training experienced a mean increase in
6MWD of 50 m (20%) (65). Lung volume reduction surgery in
patients with very severe COPD has been reported to increase
6MWD by a mean of 55 m (20%) (13).
Cardiac rehabilitation in patients referred with various heart
diseases increased 6MWD by a mean of 170 m (15%) in a recent
study (66). In 25 older patients with heart failure, an angiotensin-
converting enzyme inhibitor medication (50 mg captopril per
day) improved 6MWD a mean of 64 m (39%) compared with a
mean increase of only 8% in those receiving a placebo (19).
Interpreting Single Measurements of Functional Status
Optimal reference equations from healthy population-based
samples using standardized 6MWT methods are not yet avail-
able. In one study, the median 6MWD was approximately 580
m for 117 healthy men and 500 m for 173 healthy women (50).
A mean 6MWD of 630 m was reported by another study of 51
healthy older adults (55). Differences in the population sam-
pled, type and frequency of encouragement, corridor length,
and number of practice tests may account for reported differ-
ences in mean 6MWD in healthy persons. Age, height, weight,
and sex independently affect the 6MWD in healthy adults;
therefore, these factors should be taken into consideration
when interpreting the results of single measurements made to
determine functional status. We encourage investigators to pub-
lish reference equations for healthy persons using the previ-
ously mentioned standardized procedures.
A low 6MWD is nonspecific and nondiagnostic. When the
6MWD is reduced, a thorough search for the cause of the im-
pairment is warranted. The following tests may then be help-
ful: pulmonary function, cardiac function, ankle–arm index,
muscle strength, nutritional status, orthopedic function, and
cognitive function.
Conclusions
The 6MWT is a useful measure of functional capacity targeted
at people with at least moderately severe impairment. The test
has been widely used for preoperative and postoperative eval-
uation and for measuring the response to therapeutic inter-
ventions for pulmonary and cardiac disease. These guidelines
provide a standardized approach to performing the 6MWT.
The committee hopes that these guidelines will encourage fur-
ther research into the 6MWT and allow direct comparisons
among different studies.
This statement was developed by the ATS Committee on Proficiency Stan-
dards for Clinical Pulmonary Function Laboratories.
Members of the committee are:
ROBERT O. CRAPO, M.D., Chair*
RICHARD CASABURI, PH.D, M.D.
ALLAN L. COATES, M.D.
PAUL L. ENRIGHT, M.D.*
NEIL R. MACINTYRE, M.D.
ROY T. MCKAY, PH.D.
DOUGLAS JOHNSON, M.D.
JACK S. WANGER, M.S.
R. JORGE ZEBALLOS, M.D.*
Ad Hoc Committee members are:
VERA BITTNER, M.D.
CARL MOTTRAM, R.R.T.
*Writing Committee Members
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APPENDIX
The following elements should be present on the 6MWT worksheet and report:
Lap counter:
__ __ __ __ __
__ __ __ __ __
__ __ __ __ __
Patient name: ____________________
Patient ID# ___________
Walk # ______
Tech ID: _________ Date: __________
Gender: M F
Age: ____
Race: ____
Height: ___ft ____in, ____ meters
Weight: ______ lbs, _____kg
Blood pressure: _____ / _____
Medications taken before the test (dose and time): __________________
Supplemental oxygen during the test: No
Yes, flow ______ L/min, type _____
End of Test
___:___
Baseline
Time
___:___
Heart Rate
_____
_____
Dyspnea
____
____
Fatigue
SpO2
____ %
____ (Borg scale)
____ (Borg scale)
____%
Stopped or paused before 6 minutes? No
Yes, reason: _______________
Other symptoms at end of exercise: angina
dizziness
hip, leg, or calf pain
Number of laps: ____ (60 meters)  final partial lap: _____ meters 
Total distance walked in 6 minutes: ______ meters
Predicted distance: _____ meters
Percent predicted: _____%
Tech comments:
Interpretation (including comparison with a preintervention 6MWD):

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APPENDIX 2: FUNCTIONAL CLASSIFICATION OF
PULMONARY HYPERTENSION MODIFIED AFTER THE NEW 
YORK HEART ASSOCIATION FUNCTIONAL 
CLASSIFICATION ACCORDING TO THE WHO 1998
(GALIE, 2009)
World Health Organization functional assessment classification 
Class 
I:  
Patients with pulmonary hypertension (PH) but without resulting limitation of physical 
activity. Ordinary physical activity does not cause undue dyspnea or fatigue, chest pain, 
or near syncope. 
Class 
II:  
Patients with PH resulting in slight limitation of physical activity. They are comfortable 
at rest. Ordinary physical activity causes undue dyspnea or fatigue, chest pain, or near 
syncope. 
Class 
III:  
Patients with PH resulting in marked limitation of physical activity. They are 
comfortable at rest. Less than ordinary activity causes undue dyspnea or fatigue, chest 
pain, or near syncope. 
Class 
IV:  
Patients with PH with inability to carry out any physical activity without symptoms. 
These patients manifest signs of right-heart failure. Dyspnea and/or fatigue may even be 
present at rest. Discomfort is increased by any physical activity. 

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APPENDIX 3:  BORG CR10 SCALE