Jerryhuang001/TF_network huggingface dataset:
Gene: NPAS2 (ENSG00000170485)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Known motif
Binding mode: 1 Monomer or homomultimer
Motif status: High-throughput in vitro
Reviewer 1 (Jussi Taipale) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 2 (Sam Lambert) assessment: Has known motif; binding: 1 Monomer or homomultimer
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: TF Gene_DNA-Binding: sequence-specific_DNA Binding_PMIDS:11441146
Annotated as a GO transcription factor: Yes
Gene: NPAS3 (ENSG00000151322)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Inferred motif
Binding mode: 2 Obligate heteromer
Motif status: In vivo/Misc source
Comments: Likely obligate heteromer (PMID: 9374395; PMID: 27782878).
AJ notes: PDB:5SY7 has ARNT-NPAS3 heterodimer. In the study PMID: 27782878 they also measured that both NPAS3 and NPAS1 bind with ARNT the same DNA CGTACGTGCG with 20nM kDa. However close paralog NPAS1 binds DNA just fine as a homodimer iin HT-SELEX and it's HLH-region is highly similar to that of NPAS3
Disagreement on binding: Disagree
Reviewer 1 (Matt Weirauch) assessment: Likely to be sequence specific TF; binding: 2 Obligate heteromer
Reviewer 1 comment: Looks like an obligate heteromer - see PMID 9374395 for example
Reviewer 2 (Pratyush Das) assessment: Likely to be sequence specific TF; binding: 1 Monomer or homomultimer
Reviewer 2 comment: Full length hasn't been tried
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: TF Gene Candidate_DNA-Binding: sequence-specific_Other_PMIDS:16172381;9374395;16172381;10534623;16172381;16190882
Annotated as a GO transcription factor: Yes
Gene: NPAS4 (ENSG00000174576)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Inferred motif
Binding mode: 2 Obligate heteromer
Motif status: High-throughput in vitro
Comments: Likely dimerizes with ARNT2 (PMID:24263188).
Reviewer 1 (Jussi Taipale) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 2 (Arttu Jolma) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 2 comment: A review suggests that it forms heterodimer with ARNT2 PMID:24263188; NPAS4:ARNT2
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: TF Gene_DNA-Binding: sequence-specific_DNA Binding_PMIDS:14701734
Annotated as a GO transcription factor: Yes
Gene: OLIG1 (ENSG00000184221)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Known motif
Binding mode: 1 Monomer or homomultimer
Motif status: High-throughput in vitro
Reviewer 1 (Sam Lambert) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 2 (Laura Campitelli) assessment: Has known motif; binding: 1 Monomer or homomultimer
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: TF Gene_DNA-Binding: sequence-specific; Transcription Factor Binding: tf co-factor binding_DNA Binding; TF PPI; Transactivation_PMIDS:18160645;15703389
Annotated as a GO transcription factor: No
Gene: OLIG2 (ENSG00000205927)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Known motif
Binding mode: 1 Monomer or homomultimer
Motif status: High-throughput in vitro
Comments: OLIG2 can homodimerize and heterodimerize with OLIG1 and NGN2 (PMID:15655114; PMID: 21382552).
Reviewer 1 (Sam Lambert) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 1 comment: OLIG2 can homodimerize; as well as heterodimerize with OLIG1 and NGN2 (PMID:15655114; 21382552)
Reviewer 2 (Yimeng Yin) assessment: Has known motif; binding: 1 Monomer or homomultimer
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: TF Gene_DNA-Binding: sequence-specific; Transcription Factor Binding: tf co-factor binding_TF PPI_PMIDS:14576772;15655114
Annotated as a GO transcription factor: Yes
Gene: OLIG3 (ENSG00000177468)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Known motif
Binding mode: 1 Monomer or homomultimer
Motif status: High-throughput in vitro
Reviewer 1 (Jussi Taipale) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 2 (Yimeng Yin) assessment: Has known motif; binding: 1 Monomer or homomultimer
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: Indeterminate - There is no evidence for or against this genes role as a TF___PMIDS:0;
Annotated as a GO transcription factor: No
Gene: PTF1A (ENSG00000168267)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Known motif
Binding mode: 1 Monomer or homomultimer
Motif status: High-throughput in vitro
Reviewer 1 (Matt Weirauch) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 2 (Sam Lambert) assessment: Has known motif; binding: 1 Monomer or homomultimer
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: TF Gene_DNA-Binding: sequence-specific_DNA Binding_PMIDS:2294404;11279116
Annotated as a GO transcription factor: No
Gene: SCX (ENSG00000260428)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Inferred motif
Binding mode: 1 Monomer or homomultimer
Motif status: High-throughput in vitro
Comments: SCX heterodimerizes with TCF3 (PMID: 19828133). The Taipale lab has an unpublished HT-SELEX model; both it and the inferred B1H motif seem very weak, supporting the idea that the homodimer does not bind DNA well.
AJ notes: Very similar to TCF15 which bound as a homodimer in SELEX (E coli expressed, Jolma 2015). Basic region is fine based on alignment
Disagreement on assessment: Disagree
Reviewer 1 (Jussi Taipale) assessment: Likely to be sequence specific TF; binding: 2 Obligate heteromer
Reviewer 1 notes: bHLH; check for residues required for DNA binding
Reviewer 2 (Arttu Jolma) assessment: Has known motif; binding: 2 Obligate heteromer
Reviewer 2 comment: SCX heterodimerizes with TCF3 PMID: 19828133. Taipale lab has an unpublished HT-SELEX model; both it and the indirect B1H motif seem very weak supporting idea that the homodimer does not bind DNA well
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: TF Gene Candidate_Transcription Factor Binding: tf co-factor binding; DNA-Binding: sequence-specific_Co-activation_PMIDS:10775504;15831523
Annotated as a GO transcription factor: No
Gene: SIM1 (ENSG00000112246)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Inferred motif
Binding mode: 2 Obligate heteromer
Motif status: In vivo/Misc source
Comments: Similar to SIM2, which is a likely obligate heteromer.
AJ notes: "Likely to be sequence specific TF""2 Obligate heteromer" All motifs are inferred with max 0.8 similarity SIM1 and SIM2 are very similar to each other and likely to be obligate heterodimers
Disagreement on assessment: Disagree
Reviewer 1 (Tim Hughes) assessment: Likely to be sequence specific TF; binding: 1 Monomer or homomultimer
Reviewer 1 comment: FlyFactorSurvey ortholog gave a nice motif in B1H
Reviewer 2 (Sam Lambert) assessment: Has known motif; binding: 1 Monomer or homomultimer
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: No
Annotated as a GO transcription factor: Yes
Gene: SIM2 (ENSG00000159263)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Inferred motif
Binding mode: 2 Obligate heteromer
Motif status: In vivo/Misc source
Comments: Likely obligate hetereomer (PMID: 7592839)
AJ notes: "Likely to be sequence specific TF""2 Obligate heteromer" All motifs are inferred with max 0.8 similarity SIM1 and SIM2 are very similar to each other and likely to be obligate heterodimers
Disagreement on assessment: Disagree
Reviewer 1 (Matt Weirauch) assessment: Likely to be sequence specific TF; binding: 2 Obligate heteromer
Reviewer 1 comment: Looks like an obligate hetereomer - PMID 7592839
Reviewer 2 (Sam Lambert) assessment: Has known motif; binding: 2 Obligate heteromer
Reviewer 2 comment: Binds as a dimer with ARNT2 (PMID:24263188)
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: TF Gene_DNA-Binding: non-sequence-specific; Transcription Factor Binding: tf co-factor binding_Co-activation_PMIDS:11782478
Annotated as a GO transcription factor: Yes
Gene: SOHLH1 (ENSG00000165643)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Likely to be sequence specific TF
Binding mode: 2 Obligate heteromer
Motif status: No motif
Comments: Forms SOHLH1-SOHLH2 heterodimers (PMID: 22056784).
Reviewer 1 (Arttu Jolma) assessment: Likely to be sequence specific TF; binding: 2 Obligate heteromer
Reviewer 1 comment: Forms SOHLH1-SOHLH2 heterodimers based on PMID: 22056784
Reviewer 2 (Pratyush Das) assessment: Likely to be sequence specific TF; binding: 2 Obligate heteromer
Reviewer 2 comment: PMID: 22056784  says SOHLH1 and SOHLH2 heterodimerize
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: No
Annotated as a GO transcription factor: Yes
Gene: SOHLH2 (ENSG00000120669)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Known motif
Binding mode: 1 Monomer or homomultimer
Motif status: High-throughput in vitro
Reviewer 1 (Tim Hughes) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 2 (Pratyush Das) assessment: Has known motif; binding: 1 Monomer or homomultimer
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: No
Annotated as a GO transcription factor: Yes
Gene: SREBF1 (ENSG00000072310)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Known motif
Binding mode: 1 Monomer or homomultimer
Motif status: High-throughput in vitro
Reviewer 1 (Jussi Taipale) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 2 (Matt Weirauch) assessment: Has known motif; binding: 1 Monomer or homomultimer
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: TF Gene_DNA-Binding: sequence-specific_DNA Binding_PMIDS:11829742
Annotated as a GO transcription factor: Yes
PDB structure: 1AM9
Gene: SREBF2 (ENSG00000198911)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Known motif
Binding mode: 1 Monomer or homomultimer
Motif status: High-throughput in vitro
Reviewer 1 (Sam Lambert) assessment: Has known motif; binding: 2 Obligate heteromer
Reviewer 1 comment: ChIP motifs appear to be heterodimers; and HT-SELEX motifs appear to be homodimers  
Reviewer 2 (Pratyush Das) assessment: Has known motif; binding: 2 Obligate heteromer
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: TF Gene Candidate_DNA-Binding: sequence-specific_DNA Binding_PMIDS:7903453
Annotated as a GO transcription factor: Yes
Gene: TAL1 (ENSG00000162367)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Known motif
Binding mode: 2 Obligate heteromer
Motif status: In vivo/Misc source
Notes: Only known motifs are from Transfac or HocoMoco - origin is uncertain
Comments: No motif yielded from PBMs or HT-SELEX. Binds DNA as heterodimer with TCF3 (PDB:2YPB and PDB:2YPA).
AJ notes: "Likely to be sequence specific TF" "2 Obligate heteromer" TAL1, TAL2 and LYL are very similar and have a basic region with a bulky tryptophan inserting into the cluster of the basic residues, yet at least TAL1 binds DNA well as a heterodimer with TCF3. The motifs seem like in vivo motifs except possibly for a one transfac motif. Is known to bind DNA as heterodimer with TCF3 PDB:2YPB and PDB:2YPA
Reviewer 1 (Sam Lambert) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 1 comment: Some Transfac motifs appear dubious and don't contain an apparent E-box 
Reviewer 2 (Yimeng Yin) assessment: Has known motif; binding: 1 Monomer or homomultimer
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: TF Gene_DNA-Binding: sequence-specific_Transactivation_PMIDS:8016094;8289805
Annotated as a GO transcription factor: Yes
PDB structure: 2YPB
Gene: TAL2 (ENSG00000186051)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Inferred motif
Binding mode: 2 Obligate heteromer
Motif status: In vivo/Misc source
Comments: No motif yielded from PBMs or HT-SELEX. Similar to TAL1, which binds DNA as heterodimer with TCF3 (PDB:2YPB and PDB:2YPA).
AJ notes: "Likely to be sequence specific TF" "2 Obligate heteromer" TAL1, TAL2 and LYL are very similar and have a basic region with a bulky tryptophan inserting into the cluster of the basic residues, yet at least TAL1 binds DNA well as a heterodimer with TCF3. The motifs seem like in vivo motifs except possibly for a one transfac motif. Is known to bind DNA as heterodimer with TCF3 PDB:2YPB and PDB:2YPA
Disagreement on assessment: Disagree
Reviewer 1 (Sam Lambert) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 1 comment: Based on indirect motifs
Reviewer 2 (Pratyush Das) assessment: Likely to be sequence specific TF; binding: 1 Monomer or homomultimer
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: TF Gene Candidate_DNA-Binding: sequence-specific_DNA Binding_PMIDS:8152805;11071772
Annotated as a GO transcription factor: Yes
Gene: TCF12 (ENSG00000140262)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Known motif
Binding mode: 1 Monomer or homomultimer
Motif status: High-throughput in vitro
Reviewer 1 (Sam Lambert) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 2 (Pratyush Das) assessment: Has known motif; binding: 1 Monomer or homomultimer
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: TF Gene_DNA-Binding: sequence-specific_DNA Binding_PMIDS:8163514;12826747
Annotated as a GO transcription factor: Yes
Gene: TCF15 (ENSG00000125878)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Inferred motif
Binding mode: 1 Monomer or homomultimer
Motif status: High-throughput in vitro
Reviewer 1 (Tim Hughes) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 2 (Laura Campitelli) assessment: Has known motif; binding: 1 Monomer or homomultimer
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: TF Gene Candidate_DNA-Binding: non-sequence-specific_DNA Binding_PMIDS:7597044
Annotated as a GO transcription factor: Yes
Gene: TCF21 (ENSG00000118526)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Known motif
Binding mode: 1 Monomer or homomultimer
Motif status: High-throughput in vitro
Reviewer 1 (Laura Campitelli) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 2 (Yimeng Yin) assessment: Has known motif; binding: 1 Monomer or homomultimer
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: No
Annotated as a GO transcription factor: Yes
Gene: TCF23 (ENSG00000163792)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Inferred motif
Binding mode: 1 Monomer or homomultimer
Motif status: High-throughput in vitro
Comments: Basic region is very similar to TCF21, which binds DNA as a homodimer in HT-SELEX.
AJ notes: "Likely to be sequence specific TF" "1 Monomer or homomultimer". Basic region is very similar to TCF21 that binds DNA as a homodimer in HT-SELEX
Disagreement on assessment: Disagree
Reviewer 1 (Sam Lambert) assessment: Likely to be sequence specific TF; binding: 1 Monomer or homomultimer
Reviewer 1 comment: The mouse ortholog of TCF23 (OUT; 94% Amino Acid Identity) has similar activity to inhibitory ID bHLH factors in gel-shift experiments (PMID:10652346) but appears to retain some DNA-binding activity on it's own. These inhibitory bHLH proteins can sti
Reviewer 2 (Yimeng Yin) assessment: Has known motif; binding: 1 Monomer or homomultimer
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: Indeterminate - There is no evidence for or against this genes role as a TF___PMIDS:0
Annotated as a GO transcription factor: No
Gene: TCF24 (ENSG00000261787)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Inferred motif
Binding mode: 1 Monomer or homomultimer
Motif status: High-throughput in vitro
Reviewer 1 (Sam Lambert) assessment: Likely to be sequence specific TF; binding: 1 Monomer or homomultimer
Reviewer 2 (Pratyush Das) assessment: Likely to be sequence specific TF; binding: 1 Monomer or homomultimer
CisBP considers it a TF: Yes
TFclass considers it a TF: No
TF-CAT classification: No
Annotated as a GO transcription factor: No
Gene: TCF3 (ENSG00000071564)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Known motif
Binding mode: 1 Monomer or homomultimer
Motif status: High-throughput in vitro
Reviewer 1 (Tim Hughes) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 2 (Matt Weirauch) assessment: Has known motif; binding: 1 Monomer or homomultimer
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: TF Gene_DNA-Binding: sequence-specific_DNA Binding; Transactivation_PMIDS:14627819
Annotated as a GO transcription factor: Yes
PDB structure: 2QL2
Gene: TCF4 (ENSG00000196628)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Known motif
Binding mode: 1 Monomer or homomultimer
Motif status: High-throughput in vitro
Reviewer 1 (Sam Lambert) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 2 (Pratyush Das) assessment: Has known motif; binding: 1 Monomer or homomultimer
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: TF Gene_DNA-Binding: sequence-specific_DNA Binding; TF PPI; Transactivation_PMIDS:8978694
Annotated as a GO transcription factor: Yes
Gene: TCFL5 (ENSG00000101190)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Known motif
Binding mode: 1 Monomer or homomultimer
Motif status: High-throughput in vitro
Reviewer 1 (Sam Lambert) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 2 (Yimeng Yin) assessment: Has known motif; binding: 1 Monomer or homomultimer
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: No
Annotated as a GO transcription factor: Yes
Gene: TFAP4 (ENSG00000090447)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Known motif
Binding mode: 1 Monomer or homomultimer
Motif status: High-throughput in vitro
Comments: Also can form heterodimers (PMID: 26550823).
Reviewer 1 (Matt Weirauch) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 2 (Pratyush Das) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 2 comment: Also form heterodimer; PMID: 26550823 
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: TF Gene Candidate_DNA-Binding: sequence-specific_DNA Binding_PMIDS:2123466;2833704
Annotated as a GO transcription factor: Yes
Gene: TFE3 (ENSG00000068323)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Known motif
Binding mode: 1 Monomer or homomultimer
Motif status: High-throughput in vitro
Reviewer 1 (Tim Hughes) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 2 (Arttu Jolma) assessment: Has known motif; binding: 1 Monomer or homomultimer
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: TF Gene_DNA-Binding: sequence-specific_DNA Binding_PMIDS:10707962;15994295
Annotated as a GO transcription factor: Yes
Gene: TFEB (ENSG00000112561)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Known motif
Binding mode: 1 Monomer or homomultimer
Motif status: High-throughput in vitro
Reviewer 1 (Arttu Jolma) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 2 (Sam Lambert) assessment: Has known motif; binding: 1 Monomer or homomultimer
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: Indeterminate - There is no evidence for or against this genes role as a TF___PMIDS:0
Annotated as a GO transcription factor: Yes
Gene: TFEC (ENSG00000105967)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Known motif
Binding mode: 1 Monomer or homomultimer
Motif status: High-throughput in vitro
Reviewer 1 (Arttu Jolma) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 2 (Yimeng Yin) assessment: Has known motif; binding: 1 Monomer or homomultimer
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: TF Gene Candidate_DNA-Binding: sequence-specific_DNA Binding; Transactivation_PMIDS:8336698
Annotated as a GO transcription factor: Yes
Gene: TWIST1 (ENSG00000122691)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Known motif
Binding mode: 1 Monomer or homomultimer
Motif status: In vivo/Misc source
Notes: Only known motifs are from Transfac or HocoMoco - origin is uncertain
Comments: Can form both homodimers and heterodimers with TCF3 (PMID: 16502419).
AJ notes: "Has known motif". Based on PMID: 16502419 it can form both homodimers and heterodimers with TCF3
Disagreement on binding: Disagree
Reviewer 1 (Jussi Taipale) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 2 (Matt Weirauch) assessment: Has known motif; binding: 2 Obligate heteromer
Reviewer 2 comment: tested on both PBM and SELEX; with no motif.  Probably a heteromer: PMID 16502419
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: TF Gene_DNA-Binding: sequence-specific_DNA Binding_PMIDS:12210745
Annotated as a GO transcription factor: Yes
Gene: TWIST2 (ENSG00000233608)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Known motif
Binding mode: 1 Monomer or homomultimer
Motif status: 100%ID - in vitro
AJ notes: "Has known motif" because the mouse motif is 100% similarity. Based on PMID: 16502419 it can form both homodimers and heterodimers with TCF3
Disagreement on assessment: Disagree
Reviewer 1 (Sam Lambert) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 1 comment: Indirect motif has 100% DBD Identity to mouse Twist2  (PBM motif)
Reviewer 2 (Yimeng Yin) assessment: Likely to be sequence specific TF; binding: 1 Monomer or homomultimer
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: TF Gene_Transcription Factor Binding: tf co-factor binding; DNA-Binding: sequence-specific_Other; TF PPI_PMIDS:11809751;14654692
Annotated as a GO transcription factor: No
Gene: USF1 (ENSG00000158773)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Known motif
Binding mode: 1 Monomer or homomultimer
Motif status: High-throughput in vitro
Reviewer 1 (Arttu Jolma) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 1 comment: Structure PDB:1AN4 is a homodimer
Reviewer 2 (Laura Campitelli) assessment: Has known motif; binding: 1 Monomer or homomultimer
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: TF Gene_DNA-Binding: sequence-specific_DNA Binding; Transactivation_PMIDS:9677331
Annotated as a GO transcription factor: Yes
PDB structure: 1AN4
Gene: USF2 (ENSG00000105698)
DNA-binding domain: bHLH
Is a transcription factor: Yes
TF assessment: Known motif
Binding mode: 1 Monomer or homomultimer
Motif status: High-throughput in vitro
Notes: Has a putative AT-hook
Reviewer 1 (Matt Weirauch) assessment: Has known motif; binding: 1 Monomer or homomultimer
Reviewer 2 (Sam Lambert) assessment: Has known motif; binding: 1 Monomer or homomultimer
CisBP considers it a TF: Yes
TFclass considers it a TF: Yes
TF-CAT classification: TF Gene_DNA-Binding: sequence-specific_DNA Binding_PMIDS:15242350
Annotated as a GO transcription factor: Yes