Reviewer’ s report
Title: Elevated plasma levels of lysophosphatidic acid and aberrant expression of lysopho-
sphatidic acid receptors in adenomyosis
Version: 1Date: 25 Sep 2017
Reviewer: Jemma Evans
Reviewer's report:
although the manuscript has been revised there are still a number of issues raised in the first review
which have not been addressed:1) it is indicated that the fertility status of the women withadenomyosis in the current study has been provided - I could not find this information in therevised manuscript. All that seems to have been added is information regarding their hormonalprofiles. normal hormone profiles does not provide information in fertility2) Although informationregarding the ectopic tissue has bene provided specific details requested regarding the method ofcollection are absent3) Materials and methods - immunohistochemistry. Sufficient detail to enablerepetition of the experiment is required, therefore more information regarding the antibodies, i.e.which species the primary antibodies were raised in, and the secondary antibodies, e.g. what wasused, horse anti goat, rabbit anti mouse etc, are required4) stromal cel isolation - what pore sizemesh was used - this is a critical detail in ensuring isolation of pure cell preparations5) LPAreceptor results section not revised - the reader needs to be directed to specific panels (panel A,panel B etc) within each figure in the description of results6) n-numbers are required in figurelegend for immunohistochemistry. It is accepted that representative images are presented,however, the total number of tissues examined must be indicated
Are the methods appropriate and well described?
If not, please specify what is required in your comments to the authors.
NoDoes the work include the necessary controls?
If not, please specify which controls are required in your comments to the authors.
YesAre the conclusions drawn adequately supported by the data shown?
If not, please explain in your comments to the authors.
YesAre you able to assess any statistics in the manuscript or would you recommend an
additional statistical review?If an additional statistical review is recommended, please specify what aspects require further
assessment in your comments to the editors.
I am able to assess the statistics
Quality of written English
Please indicate the quality of language in the manuscript:
AcceptableDeclaration of competing interests
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