Title: Vitamin D has therapeutic effects on obesity and hyperandrogenemia in PCOS mouse
model induced by low dose DHEA and high-fat diet
Version: 2 Date: 28 May 2024
Reviewer:  Farideh Zafari Zangeneh
Reviewer's report:
Dear Authors
Abstract:
1) In your study, there are 4 groups: control, PCO model, PCO model with low and high dose
of vitamin D. You can use the control group to compare with the PCO group, and to compare
the vitamin dose, you must have two other control groups with low and high doses. And make
sure that your analogy is correct. Then, you should have 6 groups: control without drug and with
drug low/high doses, study group without drug and with drug low/high doses.
2) In your study, there are 4 groups: control, PCO model, PCO model with low and high dose
of vitamin D. You can use the control group to compare with the PCO group, and to compare
the vitamin dose, you must have two other control groups with low and high doses. And make
sure that your analogy is correct. Then, you should have 6 groups: control without drug and with
drug low/high doses, study group without drug and with drug low/high doses.
Introduction:
1) Please use the most recent references to introduce polycystic ovary as a syndrome with
unknown etiology.
2) A little and usefully explanation about the syndrome, insulin resistance, hyper androgenic,
and their communication in women with PCOS and the obese is necessary for the reader of the
article which can then think and comment on the results of the study.
Method:
1) Please provide a reference for the mice modeling PCOS.
2) Control group fed a normal diet, and were injected with placebo. What was the placebo?
3) For a better comparison, you should have 3 study groups and 3 control groups.
4) The estrous cycle in the control group had a normal pattern and in the two groups of modeling
and low dose of vitamin D, this pattern was abnormal. In the control group, you should have a
low-dose vitamin D group in order to be able to compare.
Discussion:
Your data illustrate that Vit.D has therapeutic effects on obesity and hyperandrogenemia in
PCOS, which may occur through regulation of liver metabolism. However, over dose of Vit.D
is poisonous.
I think that this type of animal research is important not in the direction of achieving a beneficial
cure, but in the etiology of polycystic ovary syndrome. Therefore, vitamin deficiency in men
and women is important and can be compensated by diet, except in the pathological deficiency
of this vitamin.
I think that this type of animal research is important not in the direction of achieving a benifical
cure, but in the etiology of polycystic ovary syndrome. Therefore, vitamin deficiency in men
and women is important and can be compensated by diet, except in the pathological deficiency
of this vitamin.
Material selection is a very important step in polycystic ovary modeling in animals. The use
of obesity diet in modeling increases the complexity of the work.  In clinical studies, body
mass index in women with PCOS is very important for choosing the sample size and causes the
separation of obese patients in the PCOS study.
The lower the complexity in the research process, the easier it will be to achieve the research
objectives.