Title: Urine Metabolomics for Assessing Fertility-sparing Treatment Efficacy in Endometrial
Cancer: A Non-invasive Approach using Ultra-performance Liquid Chromatography Mass
Spectrometry
Version: 1 Date: 19 Aug 2023
Reviewer's report:
Dear [Author],
I hope this message finds you well. I wanted to take a moment to share with you the
valuable feedback provided by our reviewers regarding your manuscript titled "Non-invasive
Effect Evaluation for Fertility-sparing Treatment of Endometrial Cancer based on Ultra-
performance Liquid Chromatography Mass Spectrometry Urine Metabolomics." The reviewers
have provided insightful suggestions to enhance the clarity, impact, and overall quality of your
work.
Based on their feedback, here are the key recommendations for your consideration:
The title "Non-invasive Effect Evaluation for Fertility-sparing Treatment of Endometrial Cancer
based on Ultra-performance Liquid Chromatography Mass Spectrometry Urine Metabolomics"
could be streamlined a bit for clarity and conciseness. Here's a suggestion:
"Urine Metabolomics for Assessing Fertility-sparing Treatment Efficacy in Endometrial Cancer:
A Non-invasive Approach using UPLC-MS"
Abstract:
- Include details about sample selection, age range, and eligibility criteria.
- Explain the significance of ultraperformance liquid chromatography mass spectrometry
(UPLC-MS).
- Elaborate on the relevance of metabolic pathways and biomarkers.
- Clarify the selection and relevance of chosen biomarkers.
- Briefly explain statistical methods used for validation.
- Mention potential clinical implications.
- Include future research directions.
- Clarify abbreviations and technical terms.
Introduction:
- Provide more context and direct quotes from previous studies.
- Transition smoothly to metabolomics' relevance.
- Elaborate on specific challenges of fertility-sparing treatment.
- Clearly highlight the research gap and contribution.
- Explain why urine metabolomics is sensitive and relevant.
- Connect your study to the mentioned gaps and challenges.
- End with a concise hypothesis/objective statement.
Materials and Methods:
- Explain the rationale behind inclusion and exclusion criteria.
- Define treatment response terms clearly.
- Elaborate on urine sample collection and preparation.
- Provide a brief explanation of UPLC-MS significance.
- Explain parameters for mass spectrometry.
- Clarify the purpose and significance of QC samples.
- Elaborate on data processing and significance of cutoff values.
- Explain ROC analysis and cross-validation briefly.
- Provide citations for methodologies and tools.
- Restructure the section for logical flow.
Results:
- Consider tabular presentation for demographic and clinical data.
- Provide explanations for introduced figures.
- Specify statistical tests and p-values for comparisons.
- Discuss pathway analysis' biological implications.
- Explain the rationale behind chosen biomarkers.
- Interpret AUC values and sensitivity/specificity in ROC analysis.
- Discuss clinical implications and practical utility.
Discussion:
- Relate metabolic changes to treatment effectiveness.
- Provide a biological interpretation of altered pathways.
- Discuss interplay between metabolic pathways.
- Compare findings with other relevant studies.
- Address study limitations and confounding factors.
- Discuss the need for larger-scale studies and validation.
- Discuss ethical considerations related to findings.
Conclusion:
- Summarize major findings and implications.
- Discuss practical and clinical implications.
- Suggest future research prospects.
- Include potential ethical considerations.
- Acknowledge any limitations or biases.
References:
- Use accurate in-text citations.
- Maintain consistent formatting and style.
- Use standard journal name abbreviations.
- Include volume, issue, and page numbers for journal articles.
- Consider including DOI or direct links for online resources.
- Ensure consistency in capitalization.
- Include publisher information for books and chapters.
- Add DOI or PMID for recent publications.
- Provide full URLs for online resources.
- Double-check accuracy of references' details.
I believe that by addressing these suggestions, your manuscript will significantly improve in
terms of clarity, impact, and overall readability. Your dedication to making these refinements
will undoubtedly contribute to the advancement of the field.
Please don't hesitate to reach out if you have any questions or need further clarification on any
of the recommendations provided. We are excited about the potential of your work and look
forward to your revised manuscript.
Thank you for your hard work and commitment to excellence.