Reviewer’s report
Title: 
Variations in biomarkers of dyslipidemia and dysbiosis during the
menstrual cycle - a pilot study in healthy volunteers
Version: 
0 
Date: 
12 Mar 2021
Reviewer's report:
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Please overwrite this text when adding your comments to the authors.I read with
great interest the manuscript, which falls within the aim of this Journal. In my
honest opinion, the topic is interesting enough to attract the readers' attention.
Nevertheless, authors should clarify some points and improve the discussion, as
suggested below.
Authors should consider the following recommendations:
- Manuscript should be further revised in order to correct some typos and
improve style.
- To date, accumulating evidence suggests a robust interplay between B vitamins
and omega-3 polyunsaturated fatty acids (authors may refer to: PMID:
32024302; PMID: 27916823), which gained increasing attention especially for
the risk of degenerative disorders, such as cardiovascular disease and cognitive
decline. I would suggest to stress, at least briefly, how this element may affect
dyslipidemia and dysbiosis and play a role for the fine-regulated events during
the menstrual cycle.
- Do the authors think that vitamin D levels, which are known to play a pivotal
role at endometrial level especially for implantation/decidualization, may affect
at least partially the dysbiosis during the menstrual cycle? I would recommend to
discuss this point, referring to: PMID: 26000308; PMID: 29028072.
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Is the study design appropriate to answer the research question (including the use
of appropriate controls), and are the conclusions supported by the evidence
presented?
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