Reviewer’s report
Title: 
Joint effect of particulate matter and cigarette smoke on women’s sex
hormones
Version: 
1 
Date: 
17 Dec 2021
Reviewer's report:
Dear respectable Editor
Thank you to give me the opportunity to review the manuscript entitled: Joint
effect of particulate matter and cigarette smoke on women's sex hormones.
The study aimed to assess whether exposure to particulate matter (PM10) and
tobacco smoke have an impact on menstrual profiles of 17
β
-estradiol (E2) and
progesterone (P) and the E2/P ratio.
The study concluded that PM10 and tobacco smoke affect ovarian hormones
independently and do not interact with each other. Both exposures appear to
have estrogenic effects even though women's susceptibility to these effects
differs across the menstrual cycle for PM10. Authors propose that the hormonal
mechanisms are involved in observed relationships between air pollution and
smoke with women's reproductive health.
The manuscript presents an interesting subject and well-designed and it
improved too much after the authors revision.
I have no further suggestions and it can be accepted for publication. 
Regards 
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Is the study design appropriate to answer the research question (including the use
of appropriate controls), and are the conclusions supported by the evidence
presented?
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Are the methods sufficiently described to allow the study to be repeated?
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Are the images in this manuscript (including electrophoretic gels and blots) free
from apparent manipulation?
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