Reviewer ’s report  
 
Title: A case report and follow -up o f the first live birth after heterotopic transplantation of 
cryopreserved ovarian tissue in Eastern Europe   
 
Version: 0 Date: 20 Feb 2019  
 
Reviewer: Seido Takae  
 
Reviewer's report:  
 
Summary  
Authors have described first case report of live birth from transplanted ovarian tissue in East 
Europe.  
 
Dear authors  
Thank you for submission your manuscript which have described about the first case report of 
live birth from transplanted ovarian tissue in East Europe. This paper include very important 
information about the information of live birth from ovarian tissue w hich was received 
transplanted into heterotopic position. But there are several issues to publish on the journal.  
 
Major  
1, Case presentation/Patients  
Could you describe the ovarian reserve assessment of this case including AMH levels before and 
after chem otherapy.  
 
2, Case presentation/Ovarian tissue cryopreservation  
What is the indication for ovarian tissue cryopreservation (OTC)? In generally, OTC is selected 
as fertility preservation (FP) for child patients and patients who don't enough time for FP.  
 
3, Case presentation/Ovarian tissue transplantation  
Why did you select the heterotopic ovarian tissue transplantation (OTT)?? Almost live birth 
cases are orthotopic OTT cases.  
 
4, Case presentation/Ovarian tissue transplantation  
Could you add the some US ima ge or CT images to prove the follicle developments on 
transplanted site?  
 
5, Case presentation/Ovarian graft stimulation and fertility treatment  
Why did you select the antagonist protocol? In generally, short protocol is suitable for patients 
who have low ovarian reserve.  
 
6, Case presentation/ the recovery of endogenous ovarian function  
Some of premature ovarian insufficiency patients shows recovering menstruation cycle. But it is 
not recover of ovarian function.  
 
7, Fig 2  
This fig is too abstract to under stand well this case.  
 
8, Fig 3  
This fig does not make the scientific mean.  
 
9, Fig 1  
This fig is too rough to understand. And specific years should be hidden, but describe on a 
monthly basis is needed. Please change the figure appropriately.   
Are the methods appropriate and well described?  
If not, please specify what is req uired in your comments to the authors.  
 
Yes 
 
  
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
 
Unable to assess  
 
Are the conclusions drawn adequately supported by the data shown?  
If not, please explain in your comments to the authors.  
 
No 
 
Are you able to assess any statistics in the manuscript or would you recommend an  
additional statistical review?  
If an additional statistical review is recommended, please specify what aspects requ ire further  
assessment in your comments to the editors.  
 
Not relevant to this manuscript  
 
Quality of written English  
Please indicate the quality of language in the manuscript:  
 
Acceptable  
 
Declaration of competing interests  
Please complete a declaration of competing interests, considering the following questions:  
 
1. Have you in the past five years received reimbursements, fees, funding, or salary from an  
organisation that may in any way gain or lose financially from the publi cation of this  
manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares in an organisation that may in any way gain or lose  
financially from the publication of this manuscript, either now or in the future?  
 
3. Do you hold or are you curr ently applying for any patents relating to the content of the  
manuscript?  
 
4. Have you received reimbursements, fees, funding, or salary from an organization that 
holds  or has applied for patents relating to the content of the manuscript?  
 
5. Do you have any  other financial competing interests?  
 
6. Do you have any non -financial competing interests in relation to this paper?  
 
If you can answer no to all of the above, write 'I declare that I have no competing interests' 
below.  If your reply is yes to any, please give details below.  
 
I don't have any COI which need to declare . 
 
I agree to the open peer review policy of the journal. I understand that my name will be included  
on my report to the authors and, if the manuscript is accepted for publication, my named rep ort 
including any attachments I upload will be posted on the website along with the authors'  
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CC-BY license ( http://creativecommons.org/licenses/by/4.0/ ). I understand that any comments  
which I do not wish to be included in my named report can be included as confidential comments  
to the editors, which will not be published.  
 
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