Reviewer’s report
Title: 
Applicability of polygenic risk scores in endometriosis clinical
presentation
Version: 
3 
Date: 
19 Mar 2022
Reviewer's report:
Thank you for giving me the chance to review this paper on applicability of
polygenic risk scores in endometriosis clinical presentation.
Despite good overall merit and novelty of the PRS applicability in endometriosis
patients I have some comments:
- please include some references to justify 
this sentence "However, retrograde
menstruation
has been seen in up to 90% of women [4], which indicates that other
mechanisms, such as genetic,
immunological and environmental factors are involved in the development of
endometriosis" (e.g. doi: https://doi.org/10.1016/j.jmig.2012.03.005)
- please describe in the materials section how patients with endometriosis was
included: signs of endometriosis at gyn examination and/or imaging and/or
previous surgical diagnosis? please modify the limitations of the study
accordingly.
- please describe better inclusion and exclusion criteria and list primary and
secondary aims to better clarify them
- please discuss in the discussion section on the role of other possible conditions
influencing bowel symptoms 
such as microbiome, small bowel permeability,
perineal dysfunction (eg. doi: 10.23736/S2724-606X.21.04788-2; doi:
10.1080/09513590.2020.1766440; doi: 10.1002/ijgo.14088)
- how many patients underwent previous surgery for DIE? please discuss about
functional complications of conservative or radical bowel procedures for DIE
(eg. doi: 10.1016/j.jmig.2018.08.031; doi: 10.1111/ajo.12358)
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Is the study design appropriate to answer the research question (including the use
of appropriate controls), and are the conclusions supported by the evidence
presented?
Yes
Are the methods sufficiently described to allow the study to be repeated?
Yes
Is the use of statistics and treatment of uncertainties appropriate?
Yes
Is the presentation of the work clear?
Yes
Are the images in this manuscript (including electrophoretic gels and blots) free
from apparent manipulation?
Yes