Reviewer’s report
Title: 
Can TSH level and premenstrual spotting constitute a non-invasivemarker
for the diagnosis of endometriosis?
Version: 
0 
Date: 
08 Jun 2021
Reviewer's report:
I read with great interest the manuscript, which falls within the aim of this
Journal. In my honest opinion, the topic is interesting enough to attract the
readers' attention. Nevertheless, authors should clarify some points and improve
the discussion, as suggested below.
Authors should consider the following recommendations:
- Manuscript should be further revised in order to correct some typos and
improve style.
- Accumulating evidence suggests that immune cells, adhesion molecules,
extracellular matrix metalloproteinase and pro-inflammatory cytokines
activate/alter peritoneal microenvironment, creating the conditions for
differentiation, adhesion, proliferation and survival of ectopic endometrial cells. I
would discuss these points in the light of new theories about the pathogenesis of
endometriosis, referring to: PMID: 26739265; PMID: 28100109.
- Do the authors think that there could be an increased risk of ovarian cancer in
women affected by endometriosis, especially taking into account the different
types of the disease? It would appropriate to discuss this topic, at least briefly,
referring to: PMID: 31745637.
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Is the study design appropriate to answer the research question (including the use
of appropriate controls), and are the conclusions supported by the evidence
presented?
Yes
Are the methods sufficiently described to allow the study to be repeated?
Yes
Is the use of statistics and treatment of uncertainties appropriate?
Yes
Is the presentation of the work clear?
Yes
Are the images in this manuscript (including electrophoretic gels and blots) free
from apparent manipulation?
Yes