Reviewer ’s report  
 
Title: Frequency and genotypes of Chlamydia trachomatis in pa tients attending the obstetrics 
and gynecology clinics in Jalisco, Mexico and correlation with sociodemog raphic, behavioral, 
and biological factors  
 
Version: 0 Date: 29 Mar 2017  
 
Reviewer: Silvia Bianchi  
 
Reviewer's report:  
 
Casillas -Vega et al report a study on the frequency and genotypes of Chlamydia trachomatis in 
patients attending the  obstetrics and gynecology clinics in Jalisco (Mexico) and the correlation of 
this infection with sociodemographic, behavioral and biological factors. The topic is not 
innovative in itself, even if there is a lack of data in the area involved in this study . For this 
reason the final results of this study may be of some interest to the scientific community.  
 
 
The general presentation of the manuscript is linear but some points require attention and 
revision by the Authors.  
 
 
This reviewer thinks that deep attent ion could be done on "material and methods" section.  
 
 
You have to better describe the experiments that you have done and described. For examp le: 
 
 
Lines 125 -134: how long is the fragment amplified?  
 
 
Lines 141 -142: are you sure of the amplification condition he re reported?  
 
 
This reviewer thinks it is necessary that the Authors explai n why have decided to use three 
different methods for the detection of infection. What is  the reference method? The gold 
standard? The three methods are concordant or discordant? It i s possible to analyze the 
concordance / discordance of the results in relation to the characteristics of the study population?   
 
It necessary to comment about these differences.  
 
 
 
 
 
Are the methods appropriate and well described?  
If not, please specify what is required in your comments to the authors.  
 
No 
 
Does the work include the necessary controls?  
If not, please specify which controls are required in your comments to the authors.  
 
Unable to assess  
 
Are the conclusions drawn adequately supported by the data shown?  
If not, please explain in your comments to the authors.  
 
No 
 
Are you able to assess any statistics in the manuscript or would you recommend an  
additional statistical review?  
If an additional statistical review is recommended, please speci fy what aspects require further 
assessment in your comments to the editors.  
 
I am able to assess the statistics  
 
Quality of written English  
Please indicate the quality of language in the manuscript:  
 
Acceptable  
 
Declaration of competing interests  
Please complete a declaration of competing interests, considering the following questions:  
 
1. Have you in the past five years received reimbursements, fees, funding, or salary f rom an  
organisation that may in any way gain or lose financially from the publication of this  
manuscript, either now or in the future?  
 
2. Do you hold any stocks or shares in an organisation that may in any way gain or lose  
financially from the publication of this manuscript, either now or in the future?  
 
3. Do you hold or are you currently applying for any patents relating to the content of the  
manuscript?  
 
4. Have you received reimbursements, fees, funding, or salary from an organization that 
holds  or has ap plied for patents relating to the content of the manuscript?  
 
5. Do you have any other financial competing interests?  
 
6. Do you have any non -financial competing interests in relation to this paper?  
If you can answer no to all of the above, write 'I declare that I have no competing interests' 
below.  If your reply is yes to any, please give details below.  
 
I declare that I have no co mpeting interests  
 
I agree to the open peer review policy of the journal. I understand that my name will be included  
on my report t o the authors and, if the manuscript is accepted for publication, my named report  
including any attachments I upload will be posted on the website along with the authors'  
responses. I agree for my report to be made available under a n Open Access Creative Commons  
CC-BY license ( http://creativecommons.org/licenses/by/4.0/ ). I understand that any comments  
which I do not wish to be included in my named report can be included as  confidential comments  
to the editors, which will not be published.  
 
I agree to the open peer review policy of the journal