JerMa88/BMC_Womens_Health_Peer_Reviews huggingface dataset:
Title: Prognostic Impact of PTK6 Expression in Triple Negative Breast Cancer
Version: 1 Date: 05 Jun 2023
Reviewer's report:
PEER REVIEWER ASSESSMENTS:
OBJECTIVE - Full research articles: is there a clear objective that addresses a testable research
question(s) (brief or other article types: is there a clear objective)?
Yes - there is a clear objective
DESIGN - Is the current approach (including controls and analysis protocols) appropriate for
the objective?
Yes - the approach is appropriate
EXECUTION - Are the experiments and analyses performed with technical rigor to allow
confidence in the results?
Yes - experiments and analyses were performed appropriately
STATISTICS - Is the use of statistics in the manuscript appropriate?
No - there are issues with the statistics in the study
INTERPRETATION - Is the current interpretation/discussion of the results reasonable and not
overstated?
No - there are major issues
OVERALL MANUSCRIPT POTENTIAL - Is the current version of this work technically
sound? If not, can revisions be made to make the work technically sound?
Maybe - with major revisions
PEER REVIEWER COMMENTS:
GENERAL COMMENTS: This is an interesting study showing some prognostic influence for
PTK6. The authors use a standard immunohistochemical technique and a retrospective design.
There are however certain limitations:
1/ The survival analysis is not clear neither the discussion about it. It is odd to have statistical
significance on the whole group and not in the subgroups. A multivariate analysis with all factors
is necessary.
2/ No discussion in previous similar studies is provided.
3/ It is overstated to suggest that it promotes tumor development given the absence of any
functional studies. The authors should just present their results.
4/ The positive/negative group should be defined also in tables/figures.
5/ the statistical test used in every parametre should be defined (fisher..other?)
REQUESTED REVISIONS:
1/ The survival analysis is not clear neither the discussion about it. It is odd to have statistical
significance on the whole group and not in the subgroups. A multivariate analysis with all factors
is necessary.
2/ No discussion on previous similar studies is provided.
3/ It is overstated to suggest that it promotes tumor development given the absence of any
functional studies. The authors should just present their results.
4/ The positive/negative group should be defined also in tables/figures.
5/ the statistical test used in every parametre should be defined (fisher..other?)
Title: Prognostic Impact of PTK6 Expression in Triple Negative Breast Cancer
Version: 2 Date: 28 Aug 2023
Reviewer's report:
"PEER REVIEWER ASSESSMENTS:
OBJECTIVE - Full research articles: is there a clear objective that addresses one or several
testable research questions? (Brief or other article types: is there a clear objective?)
Yes - there is a clear objective
DESIGN - Is the current approach (including controls and analysis protocols) appropriate for
the objective?
Yes - the approach is appropriate
EXECUTION - Are the experiments and analyses performed with sufficient technical rigor to
allow confidence in the results?
Yes - experiments and analyses were performed appropriately
STATISTICS - Is the use of statistics in the manuscript appropriate?
Yes - appropriate statistical analyses have been used in the study
INTERPRETATION - Is the current interpretation/discussion of the results reasonable and not
overstated?
No - there are minor issues
OVERALL MANUSCRIPT POTENTIAL - Has the author addressed your concerns sufficiently
for you to now recommend the work as a technically sound contribution? If not, can further
revisions be made to make the work technically sound?
Probably - with minor revisions
PEER REVIEWER COMMENTS:
GENERAL COMMENTS: The authors have considerably improved their manuscript with
modifications being intergrated in the text.
REQUESTED REVISIONS:
The authors mention in the introduction that several preclinical studies (no ref gievn) exist on
the subject in breast cancer but they fail to present them precisely in the discussion.
Also, the limitations of the study should be explained."
Title: Prognostic Impact of PTK6 Expression in Triple Negative Breast Cancer
Version: 2 Date: 13 Sep 2023
Reviewer:  Tarek Tawfik Amin
Reviewer's report:
BMC Women's Health
Title: "Prognostic Impact of PTK6 Expression in Triple Negative Breast Cancer".
Comments to the authors:
General comments:
- This manuscript does not provide a clear statement about the type of study design to followed.
- No clear sample size calculation, the study based on enrolling whatever available patients from
the study center?
- There is no any statement about matching of the LNM+ and LNM- groups in relation to
prognostic confounders.
- How possible confounders and other parameters were controlled and adjusted.
- The correlation mentioned in the study was based on the Chi-square testing (including
Fisher Exact) while other statistical methods should be followed. Chi-square is non-specific
nonparametric test and it does provide the direction or the strength of the possible association.
- There is no clear hypotheses
- Displaying of the results is greatly impartial and mixed with justification of the results and
never know whether it is results or discussion section.
Introduction section:
- Trails of management to inhibit (block) or modulate the hypothesized prognostic marker should
be mentioned as well as the factors contributing to the etiology of increasing PTK6 in Triple +
breast cancer with metastasis?
Methods:
- What are the values of the scoring system (for example HER2 staining) in interpreting the
survival analysis?
- Reference is required for paragraph: line 113-116.
- How survival analysis was conducted (dependent, independent, time-event, confounders and
models used) how the data required for the models were collected (death for example)
Results:
- Include numbers along with % in expressing your results.
- What other factors would be predicting metastasis in unadjusted survival model?
- Figure 2: Box and plot, what IRS score? And what type of test used to determine significance?
- Survival curves: not only the P value should be included, other parameters should be included.
- All tables: abbreviations should be mentioned clearly at the footnote, Hazard ratios should be
reported and expressed in the results section with proper interpretation.
Title: Prognostic Impact of PTK6 Expression in Triple Negative Breast Cancer
Version: 3 Date: 26 Oct 2023
Reviewer's report:
PEER REVIEWER ASSESSMENTS:
OBJECTIVE - Full research articles: is there a clear objective that addresses one or several
testable research questions? (Brief or other article types: is there a clear objective?)
Yes - there is a clear objective
DESIGN - Is the current approach (including controls and analysis protocols) appropriate for
the objective?
Yes - the approach is appropriate
EXECUTION - Are the experiments and analyses performed with sufficient technical rigor to
allow confidence in the results?
Yes - experiments and analyses were performed appropriately
STATISTICS - Is the use of statistics in the manuscript appropriate?
Yes - appropriate statistical analyses have been used in the study
INTERPRETATION - Is the current interpretation/discussion of the results reasonable and not
overstated?
Yes - the author's interpretation is reasonable
OVERALL MANUSCRIPT POTENTIAL - Has the author addressed your concerns sufficiently
for you to now recommend the work as a technically sound contribution? If not, can further
revisions be made to make the work technically sound?
Yes - current version is technically sound
PEER REVIEWER COMMENTS:
GENERAL COMMENTS: The authors improved their text according to comments.
Title: Thyroid Hormones and Ovarian Reserve: A Comprehensive Study of Women Seeking
Infertility Care
Version: 1 Date: 16 Jun 2023
Reviewer's report:
- Please explain the grouping in the working method section
- The results section needs to be rewritten.
At the beginning of the results section, please give a brief description(  the total number of
samples should be determined) and then refer to the reference.
- It is suggested to explain the results and then refer to the table number.
- In the discussion of sentences, it needs to be edited like this sentence" With 225 infertile
women, a retrospective study was performed by Weghofer et al.
Title: Thyroid Hormones and Ovarian Reserve: A Comprehensive Study of Women Seeking
Infertility Care
Version: 1 Date: 19 Jun 2023
Reviewer:  Giorgio Bogani
Reviewer's report:
I read with great interest the Manuscript titled " Thyroid Hormones and Ovarian Reserve: A
Retrospective Study of 1443 Women Seeking Infertility Care" which falls within the aim of the
Journal.
Although the manuscript can be considered already of good quality, I would suggest following
recommendations:
- I suggest a round of language revision, in order to correct few typos and improve readability.
- I would recommend to add further elements aimed to discuss the effect of thyroid function on
assisted reproduction outcomes in euthyroid (subclinical) infertile women (authors may refer to:
PMID: 36299456; PMID: 36477597).
Because of these reasons, the article should be revised and completed. Tables are clear and
interesting. Considering all these points, I think it could be of interest to the readers and, in my
opinion, it deserves the priority to be published after minor revisions.
Title: Thyroid Hormones and Ovarian Reserve: A Comprehensive Study of Women Seeking
Infertility Care
Version: 3 Date: 08 Aug 2023
Reviewer:  Giuseppe Gullo
Reviewer's report:
Dear authors,
I read with great interest the manuscript, which falls within the aim of this Journal. In my honest
opinion, the topic is interesting enough to attract the readers’ attention. Nevertheless, authors
should clarify some points and improve the discussion, as suggested below.
Authors should consider the following recommendations:
In my opinion you have to improve the paper refering to the updated literature wihin natraceutical
supplementation as inositols as well to how is important to have a good thyroid study and
to follow the Neonatal Outcomes and Long-Term Follow-Up of Children Born from in vitro
fertilization programm
I ALSO SUGGEST TO REFER TO THE PSYCOLOGICAL IMPILCATION IN THESE PTS
THAT UNDERGOING TO IVF TREATMENTS and how artificial intelligence can be helpfull
to the IVF specialist.
i SUGGEST TO READ AND CITE THESE ARTICLES
Myo-inositol: from induction of ovulation to menopausal disorder management
D-chiro-inositol induces ovulation in non-polycystic ovary syndrome (Pcos), non-insulin-
resistant young women, likely by modulating aromatase expression: A report of 2 cases
Inositols administration: further insights on their biological role
The Future Is Coming: Artificial Intelligence in the Treatment of Infertility Could Improve
Assisted Reproduction Outcomes—The Value of Regulatory Frameworks
Neonatal Outcomes and Long-Term Follow-Up of Children Born from Frozen Embryo, a
Narrative Review of Latest Research Findings
The Role of Cell and Gene Therapies in the Treatment of Infertility in Patients with Thyroid
Autoimmunity
Impact of assisted reproduction techniques on the neuro-psycho-motor outcome of newborns:
a critical appraisal
Psychological variables in medically assisted reproduction: a systematic review
Title: Thyroid Hormones and Ovarian Reserve: A Comprehensive Study of Women Seeking
Infertility Care
Version: 3 Date: 14 Aug 2023
Reviewer's report:
1- The title should be corrected. Remove the number of women studied from the title.
2- How did the authors determine the sample size?
3- In Statistical Analysis (line 18-20), authors said “they are described with median ±
interquartile range (IQR). Qualitative parameters are described by absolute frequencies.”. First
note that “median ± interquartile” is not true, it should be “median (interquartile)”.  Moreover
indicate in which table “median ± interquartile” has been reported?
4- In the statistical analysis section, it is mentioned that Mann-Whitney-U post-test was used.
It should be noted that the appropriate post-hoc test that should follow a Kruskal-Wallis test, is
"Dunn’s test", not Mann-Whitney.
5- The results of the "Pearson's product-moment correlation" that mentioned in "Statistical
Analysis" section are reported in which table?
6- The footnote of Table 1 is confusing.
7- In footnote of Tables should be explained what are the values of the table (mean, standard
deviation or ...). Also, the test that P-values were derived from, should be given in the footnote.
8- The results section is confusing. It is suggested to be revised and rewritten.
9- The manuscript has many spelling and grammatical errors and needs to be revised.
Title: Thyroid Hormones and Ovarian Reserve: A Comprehensive Study of Women Seeking
Infertility Care
Version: 3 Date: 26 Aug 2023
Reviewer's report:
The manuscript needs further analysis to prove the hypothesis of the study. The enclosed
comments may be helpful for the improvement of the manuscript.
1. Please write the purpose of your study specifically at the end of the introduction
2. Please add other inclusion criteria (having a history of infertility, duration of infertility)
3. Do you conduct a retrospective cohort study? Please specify it in the method section.
4. Please specify exposure and non-exposure group.
5. The study design is not clear? Cross-sectional or retrospective cohort?
6. How is the sample size estimated?
7. You state “The Shapiro-Wilk test was carried out to assess the normality of data. Continuous
parameters were found not to be normally distributed; thus”, why Pearson correlation test was
used? And I don’t see its findings.
8. It is unnecessary to report the details of each test used.
9. Please add other variables such as AFC, age, menstrual status, BMI, and type of infertility in
the regression model as predictors of TSH levels.
Title: Thyroid Hormones and Ovarian Reserve: A Comprehensive Study of Women Seeking
Infertility Care
Version: 4 Date: 16 Sep 2023
Reviewer:  Phuc Nhon Nguyen
Reviewer's report:
Dear  authors,
Thank you for  addressing all  necessary comment points.
The authors have promptly contributed to the literature the correlation of TSH and AMH values
in  their population of infertile women.
The present paper is great enough to be published.
However, the conclusion should be precisely focused.
In practice, we have found that the higher level of TSH, the lower level of AMH (in hypothyroid
disease).
The conclusion is easy to misunderstand also that the lower level of TSH  is related to the higher
level of AMH (in hyperthroidism).
Therefore, the authors should emphasize the population for thyroid bilan.
We also have not yet had a recommended guideline on the test of TSH for all infertile
women, thus, this should be highlighted. The test is indicated automatically or based on the
symptomatique patients. A systematic review study is needed to be conducted in future.
Yours sincerely,
P.N.N.
Title: Thyroid Hormones and Ovarian Reserve: A Comprehensive Study of Women Seeking
Infertility Care
Version: 4 Date: 22 Sep 2023
Reviewer's report:
Dear author, I believe that your study design is solely a cross-sectional study. If your study is a
retrospective cohort, you need to clearly define the exposed and unexposed groups. Additionally,
it is important to have a historical follow-up to ensure that the events have taken place in the
past. The duration of this follow-up should be specified. It should be noted that this study does
not meet any of the criteria for a cohort study.
Title: Thyroid Hormones and Ovarian Reserve: A Comprehensive Study of Women Seeking
Infertility Care
Version: 5 Date: 06 Oct 2023
Reviewer:  Phuc Nhon Nguyen
Reviewer's report:
Dear authors,
Thank you for your hard work.
After the second round of review process, the paper is well revised and deserves to be published
now.
Best regards,
Phuc Nhon Nguyen MD, MSc
Department of High-risk Pregnancy
Tu Du Clinical Research Unit (TD-CRU)
Tu Du Hospital , Ho Chi Minh City, Vietnam
Title: Thyroid Hormones and Ovarian Reserve: A Comprehensive Study of Women Seeking
Infertility Care
Version: 5 Date: 07 Oct 2023
Reviewer's report:
1-The explanation of determining sample size does not satisfy.
Sample size calculation is the principal component of a study, and for any type of study, the
sample size must be calculated.
2- What is "Herby generalized linear regression analysis" that is mentioned in the statistical
section? Please provide a valid reference for it.
3- Considering the large sample size and the range of age, it seems unlikely that the age is not
normal. Please check again.
4- P-values reported in the results section are not reported in Table 1.
5- The footnote of Table 1 was not written correctly.
6- At the beginning of the discussion, the authors mentioned the study on 1443 infertile women,
while the sample studied in this study was 1396!! Be careful that 47 women were excluded from
the study.
7- The manuscript has spelling and grammatical errors and needs to be revised.
Title: Thyroid Hormones and Ovarian Reserve: A Comprehensive Study of Women Seeking
Infertility Care
Version: 5 Date: 21 Oct 2023
Reviewer's report:
Thank you for this detailed, well-presented paper. I think this study will add to new knowledge
in the field of infertility research. I have a few comments and questions.
Title: I suggest changing “comprehensive” to “cross-sectional retrospective” instead as it will
help the reader understand the methodology at one glance. The term “comprehensive” does not
tell the reader much.
I did not see any exclusion criteria in this paper. There are other factors that could affect AMH
levels, such as obesity, polycystic ovary syndrome, other endocrine disorders etc. Is there any
exclusion criteria? Were the participants healthy or did they have some chronic conditions? I
also see that you have included obese women in your study. I think you might have to consider
and control for non-healthy conditions and obesity in your analysis.
Were there any studies conducted locally? I think it will be good to mention that in the paper,
whether or not there have been local studies. You could mention that this is the first local
association study that is conducted. If local studies exist, please include them in your discussion
section.
Line 52-53: The question of whether thyroid dysfunction is associated with ovarian reserve has
been studied by many researchers. – Please include references for this statement.
Methods section: When was FSH measured? On which day of the menstrual cycle? FSH levels
could vary throughout a woman’s menstrual cycle.
Limitation section: I suggest that you could include that these women might be fertile but their
infertility could be caused by solely male factors, but investigating male factors is out of the
scope for this study.
Title: Socio-demographic predictors of obesity among women in Mukono Central Division in
Central Uganda: A cross-sectional study
Version: 2 Date: 01 Feb 2023
Reviewer's report:
Line 18: Social demographic factors (give examples)
line 20:  include the theoretical framework that guided your decision to focus on the socio-
demographic factors. e.g.  https://doi.org/10.3390/ijerph192315719 indicates the various factors
were hypothesized and studied using the socio-ecological model.
Line 22: for the structured questionnaire indicate examples of data collected using it.
Line 23-32: Focus on predictors of obesity using one and best outcome variable. Since you
obtained body fat %, use that. Having many outcomes various confuses.
Line 33: you introduced new concepts i.e. Generalized and abdominal obesity. If you aim to
leave them, have them in the introduction. otherwise use only obesity or body fat.
Line 36-37: Are there any current recommendations/guidelines and interventions for weight
management? Mention how the results are critical to inform such interventions. Also, include
such guidelines/interventions in the introduction section.
Line 43-69: These two paragraphs do not talk to the introduction in the abstract. Note: The
gap you are addressing is the limited documentation of socio-demographic factors vs. the
obesity using other indicators other than BMI in Uganda context (Mukono). Focus would be to
demonstrate that other studies have done similar study using BMI but limitedly used body fat
or whatever indicator you decide to go with.
Line 74: Give more details above the study area in relation to Kampala city. i.e., name the city.
Line 83-92: Use your data to compute the power of your sample for indicator you decide to use.
Look for Gpower software to help you. Answer the question, Is the sample adequate for the
analysis using the indicator under study. Note.  you now have the data to compute the power
than project using other studies.
Line 94-97: Focus on one indicator for obesity.  If you measured body fat use that as the better
variable. It is limitedly used to define obesity in literature as you hinted in your abstract. Other
many outcome variables become confusing.
Line 115: space between Table and 1
Line 116-117:  Mention the names of the villages. reword: Majority (54%) of the respondents
lived near the city and 46% lived in villages. Note in the method section, you need to explain
what you mean by closer to the city or closer to village means. I.e., it is not specific. What is
the implication by the distance?
Line 133: The table can be improved if you focus on one indicator for obesity.
Line 142 -188: Delete and focus on improving the results of multi variate regression analysis.
Check from other papers how regression analysis is reported.
Line 189-205: Your data needs either multiple regression analysis or logistic regression analysis.
Report the significance of the models used. Check how the regression results are reported by
other papers.
Line 206-236: review after incorporating comments from above sections. Include limitations of
your study.