Reviewer’s report
Title: 
Serum Antioxidant Vitamin Concentrations and Oxidative Stress Markers
Associated with Symptoms and Severity of Premenstrual Syndrome: A
Prospective Cohort Study
Version: 
1 
Date: 
27 Sep 2020
Reviewer's report:
I received a revised manuscript entitled: " Serum Antioxidant Vitamin
Concentrations and Oxidative Stress Markers Associated with Symptoms of
Premenstrual Syndrome: A Prospective Cohort Study" for review (Reviewing a
second time). However, this manuscript still has major problems that need to be
corrected. 
1- The syndrome has several standardized questionnaires . Please explain Which
questionnaires were used to measure the severity of the symptoms of PMS and
to select women with this syndrome (screening)? (the name and all of the
characteristics). It is very important that use standard questionnaires and the
characteristics of the questionnaire should be clearly defined In Text. Please
clarify whether the questionnaire used in your study was developed for this
study or has previously been published elsewhere. If the questionnaire has been
published elsewhere, please provide a reference to it in your manuscript. if the
questionnaire was developed for this study please clarify questionnaire
characteristics (validity,.....) exactly.
2- The age range between 18-45 y is not correct. Since your participants were 20-
35 y, there is a strong reason that adopting the age of 18-45 is not correct. You
should change the age range.
3- It is better that you added new clinical trial(s ) about Iranian herbs like
Anisum or Trigonella foenum-graecum.
4- Details of sample size calculation should be explained in Text.
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Is the study design appropriate to answer the research question (including the use
of appropriate controls), and are the conclusions supported by the evidence
presented?
No
Are the methods sufficiently described to allow the study to be repeated?
No
Is the use of statistics and treatment of uncertainties appropriate?
Yes
Is the presentation of the work clear?
No
Are the images in this manuscript (including electrophoretic gels and blots) free
from apparent manipulation?
Yes